% Generated by roxygen2: do not edit by hand
% Please edit documentation in R/volcanoplot.R
\name{VolcanoPlot}
\alias{VolcanoPlot}
\title{Volcano plot}
\usage{
VolcanoPlot(
  data,
  x,
  y,
  ytrans = function(n) -log10(n),
  color_by = NULL,
  color_name = NULL,
  xlim = NULL,
  flip_negatives = FALSE,
  x_cutoff = NULL,
  y_cutoff = 0.05,
  split_by = NULL,
  split_by_sep = "_",
  label_by = NULL,
  x_cutoff_name = NULL,
  y_cutoff_name = NULL,
  x_cutoff_color = "red2",
  y_cutoff_color = "blue2",
  x_cutoff_linetype = "dashed",
  y_cutoff_linetype = "dashed",
  x_cutoff_linewidth = 0.5,
  y_cutoff_linewidth = 0.5,
  pt_size = 2,
  pt_alpha = 0.5,
  nlabel = 5,
  labels = NULL,
  label_size = 3,
  label_fg = "black",
  label_bg = "white",
  label_bg_r = 0.1,
  highlight = NULL,
  highlight_color = "red",
  highlight_size = 2,
  highlight_alpha = 1,
  highlight_stroke = 0.5,
  trim = c(0, 1),
  facet_by = NULL,
  facet_scales = "fixed",
  facet_ncol = NULL,
  facet_nrow = NULL,
  facet_byrow = TRUE,
  theme = "theme_this",
  theme_args = list(),
  palette = "Spectral",
  palcolor = NULL,
  title = NULL,
  subtitle = NULL,
  xlab = NULL,
  ylab = NULL,
  aspect.ratio = 1,
  legend.position = "right",
  legend.direction = "vertical",
  seed = 8525,
  combine = TRUE,
  nrow = NULL,
  ncol = NULL,
  byrow = TRUE,
  axes = NULL,
  axis_titles = axes,
  guides = NULL,
  design = NULL,
  ...
)
}
\arguments{
\item{data}{A data frame.}

\item{x}{A character string specifying the column name of the data frame to plot for the x-axis.}

\item{y}{A character string specifying the column name of the data frame to plot for the y-axis.}

\item{ytrans}{A function to transform the y-axis values.}

\item{color_by}{A character vector of column names to color the points by.
If NULL, the points will be filled by the x and y cutoff value.}

\item{color_name}{A character string to name the legend of color.}

\item{xlim}{A numeric vector of length 2 to set the x-axis limits.}

\item{flip_negatives}{A logical value to flip the y-axis for negative x values.}

\item{x_cutoff}{A numeric value to set the x-axis cutoff.
Both negative and positive of this value will be used.}

\item{y_cutoff}{A numeric value to set the y-axis cutoff.
Note that the y-axis cutoff will be transformed by \code{ytrans}.
So you should provide the original value.}

\item{split_by}{The column(s) to split data by and plot separately.}

\item{split_by_sep}{The separator for multiple split_by columns. See \code{split_by}}

\item{label_by}{A character string of column name to use as labels.
If NULL, the row names will be used.}

\item{x_cutoff_name}{A character string to name the x-axis cutoff.
If "none", the legend for the x-axis cutoff will not be shown.}

\item{y_cutoff_name}{A character string to name the y-axis cutoff.
If "none", the legend for the y-axis cutoff will not be shown.}

\item{x_cutoff_color}{A character string to color the x-axis cutoff line.}

\item{y_cutoff_color}{A character string to color the y-axis cutoff line.}

\item{x_cutoff_linetype}{A character string to set the x-axis cutoff line type.}

\item{y_cutoff_linetype}{A character string to set the y-axis cutoff line type.}

\item{x_cutoff_linewidth}{A numeric value to set the x-axis cutoff line size.}

\item{y_cutoff_linewidth}{A numeric value to set the y-axis cutoff line size.}

\item{pt_size}{A numeric value to set the point size.}

\item{pt_alpha}{A numeric value to set the point transparency.}

\item{nlabel}{A numeric value to set the number of labels to show.
The points will be ordered by the distance to the origin. Top \code{nlabel} points will be labeled.}

\item{labels}{A character vector of row names or indexes to label the points.}

\item{label_size}{A numeric value to set the label size.}

\item{label_fg}{A character string to set the label color.}

\item{label_bg}{A character string to set the label background color.}

\item{label_bg_r}{A numeric value specifying the radius of the background of the label.}

\item{highlight}{A character vector of row names or indexes to highlight the points.}

\item{highlight_color}{A character string to set the highlight color.}

\item{highlight_size}{A numeric value to set the highlight size.}

\item{highlight_alpha}{A numeric value to set the highlight transparency.}

\item{highlight_stroke}{A numeric value to set the highlight stroke size.}

\item{trim}{A numeric vector of length 2 to trim the x-axis values.
The values must be in the range from 0 to 1, which works as quantile to trim the x-axis values.
For example, c(0.01, 0.99) will trim the 1\% and 99\% quantile of the x-axis values.
If the values are less then 1\% or greater than 99\% quantile, the values will be set to the 1\% or 99\% quantile.}

\item{facet_by}{A character string specifying the column name of the data frame to facet the plot.
Otherwise, the data will be split by \code{split_by} and generate multiple plots
and combine them into one using \code{patchwork::wrap_plots}}

\item{facet_scales}{Whether to scale the axes of facets. Default is "fixed"
Other options are "free", "free_x", "free_y". See \code{ggplot2::facet_wrap}}

\item{facet_ncol}{A numeric value specifying the number of columns in the facet.
When facet_by is a single column and facet_wrap is used.}

\item{facet_nrow}{A numeric value specifying the number of rows in the facet.
When facet_by is a single column and facet_wrap is used.}

\item{facet_byrow}{A logical value indicating whether to fill the plots by row. Default is TRUE.}

\item{theme}{A character string or a theme class (i.e. ggplot2::theme_classic) specifying the theme to use.
Default is "theme_this".}

\item{theme_args}{A list of arguments to pass to the theme function.}

\item{palette}{A character string specifying the palette to use.
A named list or vector can be used to specify the palettes for different \code{split_by} values.}

\item{palcolor}{A character string specifying the color to use in the palette.
A named list can be used to specify the colors for different \code{split_by} values.
If some values are missing, the values from the palette will be used (palcolor will be NULL for those values).}

\item{title}{A character string specifying the title of the plot.
A function can be used to generate the title based on the default title.
This is useful when split_by is used and the title needs to be dynamic.}

\item{subtitle}{A character string specifying the subtitle of the plot.}

\item{xlab}{A character string specifying the x-axis label.}

\item{ylab}{A character string specifying the y-axis label.}

\item{aspect.ratio}{A numeric value specifying the aspect ratio of the plot.}

\item{legend.position}{A character string specifying the position of the legend.
if \code{waiver()}, for single groups, the legend will be "none", otherwise "right".}

\item{legend.direction}{A character string specifying the direction of the legend.}

\item{seed}{The random seed to use. Default is 8525.}

\item{combine}{Whether to combine the plots into one when facet is FALSE. Default is TRUE.}

\item{nrow}{A numeric value specifying the number of rows in the facet.}

\item{ncol}{A numeric value specifying the number of columns in the facet.}

\item{byrow}{A logical value indicating whether to fill the plots by row.}

\item{axes}{A string specifying how axes should be treated. Passed to \code{\link[patchwork:wrap_plots]{patchwork::wrap_plots()}}.
Only relevant when \code{split_by} is used and \code{combine} is TRUE.
Options are:
\itemize{
\item 'keep' will retain all axes in individual plots.
\item 'collect' will remove duplicated axes when placed in the same run of rows or columns of the layout.
\item 'collect_x' and 'collect_y' will remove duplicated x-axes in the columns or duplicated y-axes in the rows respectively.
}}

\item{axis_titles}{A string specifying how axis titltes should be treated. Passed to \code{\link[patchwork:wrap_plots]{patchwork::wrap_plots()}}.
Only relevant when \code{split_by} is used and \code{combine} is TRUE.
Options are:
\itemize{
\item 'keep' will retain all axis titles in individual plots.
\item 'collect' will remove duplicated titles in one direction and merge titles in the opposite direction.
\item 'collect_x' and 'collect_y' control this for x-axis titles and y-axis titles respectively.
}}

\item{guides}{A string specifying how guides should be treated in the layout. Passed to \code{\link[patchwork:wrap_plots]{patchwork::wrap_plots()}}.
Only relevant when \code{split_by} is used and \code{combine} is TRUE.
Options are:
\itemize{
\item 'collect' will collect guides below to the given nesting level, removing duplicates.
\item 'keep' will stop collection at this level and let guides be placed alongside their plot.
\item 'auto' will allow guides to be collected if a upper level tries, but place them alongside the plot if not.
}}

\item{design}{Specification of the location of areas in the layout, passed to \code{\link[patchwork:wrap_plots]{patchwork::wrap_plots()}}.
Only relevant when \code{split_by} is used and \code{combine} is TRUE. When specified, \code{nrow}, \code{ncol}, and \code{byrow} are ignored.
See \code{\link[patchwork:wrap_plots]{patchwork::wrap_plots()}} for more details.}

\item{...}{Additional arguments.}
}
\value{
A list of ggplot objects or a wrap_plots object
}
\description{
A volcano plot is a type of scatter plot that shows statistical significance (usually on the y-axis) versus magnitude of change (usually on the x-axis).
}
\examples{
\donttest{
set.seed(8525)
# Obtained by Seurat::FindMakers for the first cluster of pbmc_small
data <- data.frame(
   avg_log2FC = c(
     -3.69, -4.10, -2.68, -3.51, -3.09, -2.52, -3.53, -3.35, -2.82, -2.71, -3.16, -2.24,
     -5.62, -3.10, -3.42, -2.72, -3.23, -3.25, -4.68, 3.67, -2.66, 4.79, -2.99, 10.14,
     -1.78, -2.67, -2.26, -2.59, -3.39, 5.36, 4.56, 4.62, -2.94, -9.47, -9.12, -1.63,
     -2.77, 3.31, -1.53, -3.89, -4.21, 4.72, -2.98, -2.29, -1.41, -9.48, -4.30, 3.01,
     -1.19, -4.83, -1.35, -1.68, -1.63, -2.70, 3.86, 3.81, 7.23, -1.45, -0.92, -2.45,
     3.91, -4.45, -9.33, 3.56, 2.27, -1.60, -1.15, 11.40, -9.77, -8.32, 2.61, -1.25,
     -1.72, 10.61, 11.34, 10.02, 2.78, -3.48, -1.98, 5.86, 5.57, 4.57, 9.75, 9.97,
     10.90, 9.19, 2.93, 5.10, -1.52, -3.93, -1.95, -2.46, -0.64, 4.60, -1.82, -0.80,
     9.34, 7.51, 6.45, 5.23, 4.41, 3.60, -1.94, -1.15),
   p_val_adj = c(
     3.82e-09, 1.52e-07, 1.79e-07, 4.68e-07, 4.83e-07, 6.26e-07, 2.61e-06, 1.33e-05,
     1.79e-05, 3.71e-05, 5.21e-05, 5.36e-05, 5.83e-05, 6.66e-05, 8.22e-05, 2.89e-04,
     3.00e-04, 4.94e-04, 7.62e-04, 8.93e-04, 9.55e-04, 9.61e-04, 1.12e-03, 1.47e-03,
     1.66e-03, 1.95e-03, 2.06e-03, 3.01e-03, 3.26e-03, 4.35e-03, 4.85e-03, 5.12e-03,
     5.40e-03, 7.18e-03, 7.18e-03, 1.04e-02, 1.24e-02, 1.90e-02, 1.94e-02, 1.97e-02,
     2.09e-02, 2.13e-02, 2.25e-02, 2.61e-02, 3.18e-02, 3.27e-02, 3.69e-02, 3.80e-02,
     4.95e-02, 5.73e-02, 5.77e-02, 6.10e-02, 6.22e-02, 6.31e-02, 6.72e-02, 9.23e-02,
     9.85e-02, 1.06e-01, 1.07e-01, 1.11e-01, 1.31e-01, 1.38e-01, 1.40e-01, 1.43e-01,
     2.00e-01, 2.39e-01, 2.49e-01, 2.57e-01, 2.86e-01, 2.86e-01, 2.98e-01, 3.32e-01,
     4.15e-01, 4.91e-01, 4.91e-01, 4.91e-01, 5.97e-01, 7.11e-01, 7.59e-01, 8.38e-01,
     9.20e-01, 9.20e-01, 9.29e-01, 9.29e-01, 9.29e-01, 9.29e-01, 9.34e-01, 9.68e-01,
     1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00,
     1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00, 1.00e+00),
   gene = c(
     "HLA-DPB1", "LYZ", "HLA-DRA", "TYMP", "HLA-DPA1", "HLA-DRB1", "CST3", "HLA-DQB1",
     "HLA-DRB5", "LST1", "HLA-DQA1", "AIF1", "S100A8", "IFITM3", "HLA-DMB", "FCGRT",
     "SERPINA1", "IFI30", "S100A9", "CCL5", "GRN", "LCK", "HLA-DMA", "MS4A6A", "CTSS",
     "CFP", "FCN1", "BID", "CFD", "CD3D", "CD7", "CD3E", "LGALS2", "CD14", "SMCO4",
     "LINC00936", "HCK", "CTSW", "LGALS1", "HLA-DQA2", "LRRC25", "GZMM", "RNF130",
     "LGALS3", "S100A11", "C5AR1", "IL1B", "GZMA", "FCER1G", "MPEG1", "TYROBP", "TSPO",
     "GSTP1", "CTSB", "IL32", "CD247", "GNLY", "COTL1", "NFKBIA", "NUP214", "LAMP1",
     "FPR1", "CLEC10A", "CST7", "PRF1", "BLVRA", "PSAP", "GZMH", "EAF2", "ASGR1",
     "RARRES3", "SAT1", "LY86", "GP9", "TUBB1", "NGFRAP1", "XBP1", "SCO2", "RGS2", "GZMB",
     "HIST1H2AC", "KLRD1", "PGRMC1", "AKR1C3", "PTGDR", "IL2RB", "GYPC", "CCL4", "CD68",
     "FCER1A", "CD79B", "MS4A7", "CARD16", "ACAP1", "CD79A", "ANXA2", "TMEM40", "PF4",
     "GNG11", "CLU", "CD9", "FGFBP2", "TNFRSF1B", "IFI6"),
  pct_diff = c(
     -0.752, -0.457, -0.460, -0.671, -0.626, -0.701, -0.502, -0.619, -0.623, -0.598,
     -0.566, -0.626, -0.543, -0.566, -0.541, -0.542, -0.515, -0.489, -0.444, 0.428,
     -0.517, 0.461, -0.491, -0.410, -0.480, -0.491, -0.521, -0.491, -0.438, 0.411,
     0.411, 0.409, -0.438, -0.359, -0.359, -0.440, -0.386, 0.385, -0.332, -0.361, -0.361,
     0.364, -0.387, -0.415, -0.454, -0.308, -0.335, 0.364, -0.454, -0.309, -0.379, -0.427,
     -0.377, -0.389, 0.335, 0.315, 0.313, -0.284, -0.502, -0.309, 0.313, -0.284, -0.256,
     0.309, 0.313, -0.364, -0.406, 0.244, -0.231, -0.231, 0.281, -0.311, -0.312, 0.220,
     0.220, 0.220, 0.261, -0.232, -0.367, 0.240, 0.218, 0.218, 0.195, 0.195, 0.195, 0.195,
     0.262, 0.218, -0.288, -0.207, -0.290, -0.233, -0.367, 0.217, -0.233, -0.403, 0.171,
     0.194, 0.194, 0.194, 0.194, 0.213, -0.235, -0.292),
  group = sample(LETTERS[1:2], 104, replace = TRUE)
)
# If set, it will be used as labels if label_by is not set.
# rownames(data) <- data$gene

VolcanoPlot(data, x = "avg_log2FC", y = "p_val_adj", color_by = "pct_diff",
   y_cutoff_name = "-log10(0.05)")
VolcanoPlot(data, x = "avg_log2FC", y = "p_val_adj", color_by = "pct_diff",
   y_cutoff_name = "-log10(0.05)", label_by = "gene")
VolcanoPlot(data, x = "avg_log2FC", y = "p_val_adj", y_cutoff_name = "none",
   flip_negatives = TRUE, label_by = "gene")
VolcanoPlot(data, x = "avg_log2FC", y = "p_val_adj", y_cutoff_name = "none",
   flip_negatives = TRUE, facet_by = "group", label_by = "gene")
VolcanoPlot(data, x = "avg_log2FC", y = "p_val_adj", y_cutoff_name = "none",
   flip_negatives = TRUE, split_by = "group", label_by = "gene")
VolcanoPlot(data, x = "avg_log2FC", y = "p_val_adj", y_cutoff_name = "none",
   highlight = c("ANXA2", "TMEM40", "PF4", "GNG11", "CLU", "CD9", "FGFBP2",
   "TNFRSF1B", "IFI6"), label_by = "gene")
VolcanoPlot(data, x = "avg_log2FC", y = "p_val_adj", color_by = "pct_diff",
   y_cutoff_name = "-log10(0.05)", split_by = "group", label_by = "gene",
   palette = c(A = "Set1", B = "Dark2"))
}
}
