FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Vulimiri, SV AF Vulimiri, S. V. TI Environmental Transplacental Carcinogens: Human Health Risk Evaluation. SO ENVIRONMENTAL AND MOLECULAR MUTAGENESIS LA English DT Meeting Abstract CT 47th Annual Meeting of the Environmental-Mutagenesis-and-Genomics-Society CY SEP 24-28, 2016 CL Kansas City, MO SP Environm Mutagenesis & Genom Soc C1 [Vulimiri, S. V.] US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Washington, DC 20460 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-6692 EI 1098-2280 J9 ENVIRON MOL MUTAGEN JI Environ. Mol. Mutagen. PD SEP PY 2016 VL 57 SU 1 MA S15 BP S42 EP S42 PG 1 WC Environmental Sciences; Genetics & Heredity; Toxicology SC Environmental Sciences & Ecology; Genetics & Heredity; Toxicology GA DW4BQ UT WOS:000383587400021 ER PT J AU Wehmas, LC Hester, SD Bhat, V Chorley, BN Carswell, G Wood, CE AF Wehmas, L. C. Hester, S. D. Bhat, V Chorley, B. N. Carswell, G. Wood, C. E. TI Evaluating Quality of Aged Archival Formalin-Fixed Paraffin-Embedded Samples for RNA-Sequencing. SO ENVIRONMENTAL AND MOLECULAR MUTAGENESIS LA English DT Meeting Abstract CT 47th Annual Meeting of the Environmental-Mutagenesis-and-Genomics-Society CY SEP 24-28, 2016 CL Kansas City, MO SP Environm Mutagenesis & Genom Soc C1 [Wehmas, L. C.; Hester, S. D.; Chorley, B. N.; Carswell, G.; Wood, C. E.] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Bhat, V] Natl Sanitat Fdn Int, Ann Arbor, MI USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-6692 EI 1098-2280 J9 ENVIRON MOL MUTAGEN JI Environ. Mol. Mutagen. PD SEP PY 2016 VL 57 SU 1 MA EG-43 BP S74 EP S74 PG 1 WC Environmental Sciences; Genetics & Heredity; Toxicology SC Environmental Sciences & Ecology; Genetics & Heredity; Toxicology GA DW4BQ UT WOS:000383587400131 ER PT J AU Zavala, J Krug, JD Warren, SH Krantz, QT King, C Gavett, SH Lewandowski, M Lonneman, WA Kleindienst, TE Meier, M Higuchi, M Gilmour, MI DeMarini, DM AF Zavala, J. Krug, J. D. Warren, S. H. Krantz, Q. T. King, C. Gavett, S. H. Lewandowski, M. Lonneman, W. A. Kleindienst, T. E. Meier, M. Higuchi, M. Gilmour, M., I DeMarini, D. M. TI Two Simulated Urban Smog Atmospheres with Different Chemical Compositions Produce Similar Mutation Spectra and Mutagenic Potencies in Salmonella. SO ENVIRONMENTAL AND MOLECULAR MUTAGENESIS LA English DT Meeting Abstract CT 47th Annual Meeting of the Environmental-Mutagenesis-and-Genomics-Society CY SEP 24-28, 2016 CL Kansas City, MO SP Environm Mutagenesis & Genom Soc C1 [Zavala, J.] US EPA, ORISE, Res Triangle Pk, NC 27711 USA. [Krug, J. D.; Lewandowski, M.; Lonneman, W. A.; Kleindienst, T. E.] US EPA, NERL, Res Triangle Pk, NC 27711 USA. [Warren, S. H.; Krantz, Q. T.; King, C.; Gavett, S. H.; Higuchi, M.; Gilmour, M., I; DeMarini, D. M.] US EPA, NHEERL, Res Triangle Pk, NC 27711 USA. [Meier, M.] Carleton Univ, Dept Biol, Ottawa, ON, Canada. NR 0 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-6692 EI 1098-2280 J9 ENVIRON MOL MUTAGEN JI Environ. Mol. Mutagen. PD SEP PY 2016 VL 57 SU 1 MA XB-61 BP S76 EP S76 PG 1 WC Environmental Sciences; Genetics & Heredity; Toxicology SC Environmental Sciences & Ecology; Genetics & Heredity; Toxicology GA DW4BQ UT WOS:000383587400140 ER PT J AU Schmeltz, MT Petkova, EP Gamble, JL AF Schmeltz, Michael T. Petkova, Elisaveta P. Gamble, Janet L. TI Economic Burden of Hospitalizations for Heat-Related Illnesses in the United States, 2001-2010 SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Article DE climate change; health equity; heat-related illness; hospitalizations; economic cost; racial disparities ID EMERGENCY-DEPARTMENT VISITS; CLIMATE-CHANGE; HEALTH-CARE; US CITIES; DISPARITIES; TEMPERATURE; INEQUITIES; MORTALITY; POLICIES; IMPACTS AB Understanding how heat waves affect morbidity and mortality, as well as the associated economic costs, is essential for characterizing the human health impacts of extreme heat under a changing climate. Only a handful of studies have examined healthcare costs associated with exposures to high temperatures. This research explores costs associated with hospitalizations for heat-related illness (HRI) in the United States using the 2001 to 2010 Nationwide Inpatient Sample (NIS). Descriptive statistics of patient data for HRI hospitalizations were examined and costs of hospitalizations were reported using the all-payer inpatient cost-to-charge ratio. Costs were examined using a log-gamma model with patient and hospital characteristics included as fixed effects. Adjusted mean costs were then compared across racial groups. The mean costs of HRI hospitalizations were higher among racial/ethnic minorities compared to Whites, who accounted for almost 65% of all HRI hospitalizations. Observed differences in costs based on income, insurance, and gender were also significant. These results suggest that these populations are suffering disproportionately from health inequity, thus, they could shoulder greater disease and financial burdens due to climate change. These findings may have important implications in understanding the economic impact public health planning and interventions will have on preventing hospitalizations related to extreme heat. C1 [Schmeltz, Michael T.] US EPA, ASPPH EPA Environm Hlth Fellowship Program, Off Res & Dev, Natl Ctr Environm Assessment, Washington, DC 20001 USA. [Petkova, Elisaveta P.] Columbia Univ, Earth Inst, Natl Ctr Disaster Preparedness, New York, NY 10027 USA. [Gamble, Janet L.] US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Washington, DC 20460 USA. RP Schmeltz, MT (reprint author), US EPA, ASPPH EPA Environm Hlth Fellowship Program, Off Res & Dev, Natl Ctr Environm Assessment, Washington, DC 20001 USA. EM schmeltz.michael@epa.gov; epp2109@cumc.columbia.edu; gamble.janet@epa.gov FU U.S. Environmental Protection Agency [X3-83555301]; Association of Schools and Programs of Public Health FX This publication was supported by the Cooperative Agreement Number X3-83555301 from the U.S. Environmental Protection Agency and the Association of Schools and Programs of Public Health. The findings and conclusions of this publication do not necessarily represent the official views of the EPA or ASPPH. We would like to thank both our internal EPA reviewers and external reviewers for their thoughtful comments. NR 43 TC 0 Z9 0 U1 6 U2 6 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD SEP PY 2016 VL 13 IS 9 AR 894 DI 10.3390/ijerph13090894 PG 11 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DX9EG UT WOS:000384695800058 ER PT J AU Sullivan, JT Mcgee, TJ Langford, AO Alvarez, RJ Senff, CJ Reddy, PJ Thompson, AM Twigg, LW Sumnicht, GK Lee, P Weinheimer, A Knote, C Long, RW Hoff, RM AF Sullivan, John T. McGee, Thomas J. Langford, Andrew O. Alvarez, Raul J., II Senff, Christoph J. Reddy, Patrick J. Thompson, Anne M. Twigg, Laurence W. Sumnicht, Grant K. Lee, Pius Weinheimer, Andrew Knote, Christoph Long, Russell W. Hoff, Raymond M. TI Quantifying the contribution of thermally driven recirculation to a high-ozone event along the Colorado Front Range using lidar SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE ozone; air quality; remote sensing; lidar; air quality modeling; TOLNet ID REGIONAL-SCALE FLOWS; MOUNTAINOUS TERRAIN; BOUNDARY-LAYER AB A high-ozone (O-3) pollution episode was observed on 22 July 2014 during the concurrent Deriving Information on Surface Conditions from Column and Vertically Resolved Observations Relevant to Air Quality (DISCOVER-AQ) and Front Range Air Pollution and Photochemistry Experiment (FRAPPE) campaigns in northern Colorado. Surface O-3 monitors at three regulatory sites exceeded the Environmental Protection Agency (EPA) 2008 National Ambient Air Quality Standard (NAAQS) daily maximum 8h average (MDA8) of 75ppbv. To further characterize the polluted air mass and assess transport throughout the event, measurements are presented from O-3 and wind profilers, O-3-sondes, aircraft, and surface-monitoring sites. Observations indicate that thermally driven upslope flow was established throughout the Colorado Front Range during the pollution episode. As the thermally driven flow persisted throughout the day, O-3 concentrations increased and affected high-elevation Rocky Mountain sites. These observations, coupled with modeling analyses, demonstrate a westerly return flow of polluted air aloft, indicating that the mountain-plains solenoid circulation was established and impacted surface conditions within the Front Range. C1 [Sullivan, John T.; McGee, Thomas J.] NASA, Goddard Space Flight Ctr, Atmospher Chem & Dynam Lab, Greenbelt, MD 20771 USA. [Langford, Andrew O.; Alvarez, Raul J., II; Senff, Christoph J.] NOAA, Earth Syst Res Lab, Boulder, CO USA. [Senff, Christoph J.] Univ Colorado, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA. [Reddy, Patrick J.] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. [Thompson, Anne M.] NASA, Goddard Space Flight Ctr, Earth Sci Div, Greenbelt, MD USA. [Twigg, Laurence W.; Sumnicht, Grant K.] Sci Syst & Applicat Inc, Lanham, MD USA. [Lee, Pius] NOAA, Ctr Weather & Climate Predict, Air Resources Lab, College Pk, MD USA. [Weinheimer, Andrew] Natl Ctr Atmospher Res, Atmospher Chem Observat & Modeling Lab, POB 3000, Boulder, CO 80307 USA. [Knote, Christoph] Univ Munich, Meteorol Inst, Munich, Germany. [Long, Russell W.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Hoff, Raymond M.] Joint Ctr Earth Syst Technol, Baltimore, MD USA. [Hoff, Raymond M.] Univ Maryland, Dept Atmospher Phys, Baltimore, MD 21201 USA. RP Sullivan, JT (reprint author), NASA, Goddard Space Flight Ctr, Atmospher Chem & Dynam Lab, Greenbelt, MD 20771 USA. EM john.t.sullivan@nasa.gov RI Langford, Andrew/D-2323-2009; Thompson, Anne /C-3649-2014; Manager, CSD Publications/B-2789-2015 OI Langford, Andrew/0000-0002-2932-7061; Thompson, Anne /0000-0002-7829-0920; FU UMBC/JCET [374, 8306]; Maryland Department of the Environment (MDE) [U00P4400079]; NOAA-CREST CCNY Foundation [49173B-02]; NASA/USRA Postdoctoral Program at the Goddard Space Flight Center; NASA DISCOVER-AQ [NNX10AR39G]; Pennsylvania State University; NASA Tropospheric Chemistry Program; Tropospheric Ozone Lidar Network (TOLNet) FX Unless otherwise noted, all data used in this study can be found in the DISCOVER-AQ data archive (http://www-air.larc.nasa.gov/missions/discover-aq/), the FRAPPE data archive (http://catalog.eol.ucar.edu/frappe), or the TOLNet data archive (http://www-air.larc.nasa.gov/missions/TOLNet/). This work was supported by UMBC/JCET (task 374, project 8306), the Maryland Department of the Environment (MDE, contract U00P4400079), and NOAA-CREST CCNY Foundation (subcontract 49173B-02). This research was supported by an appointment to the NASA/USRA Postdoctoral Program at the Goddard Space Flight Center. The Platteville Nittany Atmospheric Trailer and Integrated Validation Experiment (NATIVE) operations were sponsored by NASA DISCOVER-AQ grant NNX10AR39G and the Pennsylvania State University. The authors gratefully acknowledge support provided by the NASA Tropospheric Chemistry Program and the Tropospheric Ozone Lidar Network (TOLNet). Thanks to the helpfulness and expertise of Ryan Stauffer, Hannah Halliday, and Nikolai Balashov, who worked with the NATIVE trailer at Platteville. Thanks to Debra Wicks Kollonige for providing her insight and recommendations on this work. Thanks to Kenneth Pickering, Yonhua Tang, Li Pan, and Barry Baker for their expertise in evaluating and managing the CMAQ model output. Thanks to Timothy Coleman (NOAA ESRL PSD) for providing the Greeley wind profiles. Thanks to the NOAA Physical Science Division for their continued efforts in managing the instrumentation and site coordination necessary for this work from the 300 m BAO Tower. Finally, thanks to the CDPHE for the continued efforts to obtain observations at the many remote and urban sites throughout the region used in this work. The views, opinions, and findings contained in this report are those of the author(s) and should not be construed as an official National Oceanic and Atmospheric Administration or U.S. Government position, policy, or decision. NR 34 TC 0 Z9 0 U1 8 U2 8 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD SEP PY 2016 VL 121 IS 17 BP 10377 EP 10390 DI 10.1002/2016JD025229 PG 14 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DY0YV UT WOS:000384823000008 ER PT J AU Chan, EAW Buckley, B Farraj, AK Thompson, LC AF Chan, Elizabeth A. W. Buckley, Barbara Farraj, Aimen K. Thompson, Leslie C. TI The heart as an extravascular target of endothelin-1 in particulate matter-induced cardiac dysfunction SO PHARMACOLOGY & THERAPEUTICS LA English DT Review DE Cardiac dysfunction; Particulate matter; Air pollution; Endothelin-1; Heart; Autocrine/paracrine signaling ID LONG-TERM EXPOSURE; SPONTANEOUSLY HYPERTENSIVE-RATS; SMOOTH-MUSCLE-CELLS; AIR-POLLUTION EXPOSURE; HARVARD 6 CITIES; CONCENTRATED AMBIENT PARTICLES; ACUTE MYOCARDIAL-INFARCTION; STIMULATES PHOSPHOLIPASE-C; MESSENGER-RNA EXPRESSION; NITRIC-OXIDE SYNTHESIS AB Exposure to particulate matter air pollution has been causally linked to cardiovascular disease in humans. Several broad and overlapping hypotheses describing the biological mechanisms by which particulate matter exposure leads to cardiovascular disease have been explored, although linkage with specific factors or genes remains limited. These, hypotheses may or may not also lead to particulate matter-induced cardiac dysfunction. Evidence pointing to autocrine/paracrine signaling systems as-modulators of cardiac dysfunction has increased interest in the emerging role of endothelins as mediators of cardiac function following particulate matter exposure. Endothelin-1, a well-described small peptide expressed in the pulmonary and cardiovascular systems, is best known for its ability to constrict blood vessels, although it can also induce extravascular effects. Research on the role of endothelins in the context of air pollution has largely focused on vascular effects, with limited investigation of responses resulting from the direct effects of endothelins on cardiac tissue. This represents a significant knowledge gap in air pollution health effects research, given the abundance of endothelin receptors found on cardiac tissue and the ability of endothelin-1 to modulate cardiac contractility, heart rate, and rhythm. The plausibility of endothelin-1 as a mediator of particulate matter-induced cardiac dysfunction is further supported by the therapeutic utility, of certain endothelin receptor antagonists. The present review examines the possibility that endothelin-1 release caused by exposure to PM directly modulates extravascular effects on the heart, deleteriously altering cardiac function. Published by Elsevier Inc. C1 [Chan, Elizabeth A. W.] US EPA, ORISE, Res Triangle Pk, NC 27711 USA. [Chan, Elizabeth A. W.; Buckley, Barbara] US EPA, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. [Farraj, Aimen K.; Thompson, Leslie C.] US EPA, Environm Publ Hlth Div, Res Triangle Pk, NC 27711 USA. RP Thompson, LC (reprint author), US EPA, Environm Publ Hlth Div, Res Triangle Pk, NC 27711 USA.; Thompson, LC (reprint author), US EPA, 109 TW Alexander Dr,Mail Code B105-02, Res Triangle Pk, NC 27709 USA. EM thompson.leslie@epa.gov NR 240 TC 0 Z9 0 U1 4 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0163-7258 J9 PHARMACOL THERAPEUT JI Pharmacol. Ther. PD SEP PY 2016 VL 165 BP 63 EP 78 DI 10.1016/j.pharmthera.2016.05.006 PG 16 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA DW7GH UT WOS:000383818700006 PM 27222357 ER PT J AU Li, SY Mirlekar, G Ruiz-Mercado, GJ Lima, FV AF Li, Shuyun Mirlekar, Gaurav Ruiz-Mercado, Gerardo J. Lima, Fernando V. TI Development of Chemical Process Design and Control for Sustainability SO PROCESSES LA English DT Article DE sustainability; GREENSCOPE; process control; fermentation process; process modeling ID INTEGRATED PROCESS DESIGN; ZYMOMONAS-MOBILIS; OSCILLATORY BEHAVIOR; ETHANOL FERMENTATION; CONTINUOUS CULTURES; OPTIMIZATION; INDICATORS; METHODOLOGY; KINETICS; SYSTEM AB This contribution describes a novel process systems engineering framework that couples advanced control with sustainability evaluation for the optimization of process operations to minimize environmental impacts associated with products, materials and energy. The implemented control strategy combines a biologically-inspired method with optimal control concepts for finding more sustainable operating trajectories. The sustainability assessment of process operating points is carried out by using the U.S. EPA's Gauging Reaction Effectiveness for the ENvironmental Sustainability of Chemistries with a multi-Objective Process Evaluator (GREENSCOPE) tool that provides scores for the selected indicators in the economic, material efficiency, environmental and energy areas. The indicator scores describe process performance on a sustainability measurement scale, effectively determining which operating point is more sustainable if there are more than several steady states for one specific product manufacturing. Through comparisons between a representative benchmark and the optimal steady states obtained through the implementation of the proposed controller, a systematic decision can be made in terms of whether the implementation of the controller is moving the process towards a more sustainable operation. The effectiveness of the proposed framework is illustrated through a case study of a continuous fermentation process for fuel production, whose material and energy time variation models are characterized by multiple steady states and oscillatory conditions. C1 [Li, Shuyun; Mirlekar, Gaurav; Lima, Fernando V.] West Virginia Univ, Dept Chem & Biomed Engn, Morgantown, WV 26506 USA. [Ruiz-Mercado, Gerardo J.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. RP Ruiz-Mercado, GJ (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. EM syli@mix.wvu.edu; gvmirlekar@mix.wvu.edu; Ruiz-Mercado.Gerardo@epa.gov; Fernando.Lima@mail.wvu.edu NR 39 TC 0 Z9 0 U1 10 U2 10 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2227-9717 J9 PROCESSES JI Processes PD SEP PY 2016 VL 4 IS 3 AR 23 DI 10.3390/pr4030023 PG 21 WC Engineering, Chemical SC Engineering GA DX8QC UT WOS:000384652600004 ER PT J AU Shen, GF Chen, YC Du, W Lin, N Wang, XL Cheng, HF Liu, JF Xue, CY Liu, GQ Zeng, EY Xing, BS Tao, S AF Shen, Guofeng Chen, Yuanchen Du, Wei Lin, Nan Wang, Xilong Cheng, Hefa Liu, Junfeng Xue, Chunyu Liu, Guangqing Zeng, Eddy Y. Xing, Baoshan Tao, Shu TI Exposure and size distribution of nitrated and oxygenated polycyclic aromatic hydrocarbons among the population using different household fuels SO ENVIRONMENTAL POLLUTION LA English DT Article DE PAHs derivatives; Inhalation exposure; Household air pollution; Clean cookstoves ID RESIDENTIAL WOOD COMBUSTION; CANCER-RISK ASSESSMENT; AMBIENT AIR; RURAL CHINA; EQUIVALENCY FACTORS; EMISSION FACTORS; BLOOD-PRESSURE; BOUND PAHS; NITRO-PAHS; GAS-PHASE AB Polycyclic aromatic hydrocarbons (PAHs) derivatives like nitrated and oxygenated PAHs are of growing concerns because of considerably higher toxicity and important roles during atmospheric chemical reactions. Residential solid fuel combustion is likely to be one large primary source of these pollutants in developing countries. In this study, inhalation exposure to nitrated and oxygenated PAH derivatives was evaluated among rural residents using carried samplers. The exposure levels of individual nitrated PAHs ranged from 4.04 (9-nitrated phenanthrene) to 89.8 (9-nitrated anthracene) pg/m(3), and of oxy-PAHs were 0.570 (benzo(alanthracene-7, 12-dione) to 7.99 (Benzanthrone) ng/m(3), generally higher in wood user than that in anthracite user. A majority of derivatives in particle presented in PM2.5 (80% for nitrated naphthalene and over 90% for other targets) and even fine PM1.0. Mass fractions of PAH derivatives in fine and ultra-fine particles were significantly higher than the fractions of corresponding parent PAHs, indicating more adverse health outcomes induced by these derivatives. The inhalation exposure levels for residents adopting wood gasifier burners was significantly lower than the documented results for those burning wood in typical built-in brick stoves, and comparable to those using LPG and electricity, which provided vital information for clean stove development and intervention programs. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Shen, Guofeng; Chen, Yuanchen; Du, Wei; Lin, Nan; Wang, Xilong; Cheng, Hefa; Liu, Junfeng; Tao, Shu] Peking Univ, Coll Urban & Environm Sci, Lab Earth Surface Proc, Beijing 100871, Peoples R China. [Zeng, Eddy Y.] Jinan Univ, Sch Environm, Guangzhou 510632, Guangdong, Peoples R China. [Xue, Chunyu; Liu, Guangqing] Beijing Univ Chem Technol, Dept Environm Sci & Technol, Beijing 100029, Peoples R China. [Xing, Baoshan] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA. [Shen, Guofeng] Jiangsu Acad Environm Sci, Nanjing 210036, Jiangsu, Peoples R China. [Shen, Guofeng] US EPA, NRMRL, Durham, NC 27713 USA. RP Tao, S (reprint author), Peking Univ, Coll Urban & Environm Sci, Lab Earth Surface Proc, Beijing 100871, Peoples R China.; Liu, GQ (reprint author), Beijing Univ Chem Technol, Dept Environm Sci & Technol, Beijing 100029, Peoples R China. EM gfshen12@gmail.com; gqliu@mail.buct.edu.cn; taos@pku.edu.cn RI Cheng, Hefa/A-1193-2007; OI Cheng, Hefa/0000-0003-4911-6971; SHEN, Guofeng/0000-0002-7731-5399 FU National Natural Science Foundation of China [41390240, 41130754, 41301554, 41571130010]; Ministry of Science and Technology [2015DFG92090]; Global Alliance for Clean Cookstoves, United National Foundations FX Funding for this study was supported by the National Natural Science Foundation of China (41390240, 41130754, 41301554 and 41571130010) and Ministry of Science and Technology (2015DFG92090). G. Shen and S. Tao is partly supported by the Global Alliance for Clean Cookstoves, United National Foundations. The authors would like to thank all participants in field sampling and anonymous reviewers. NR 50 TC 0 Z9 0 U1 26 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD SEP PY 2016 VL 216 BP 935 EP 942 DI 10.1016/j.envpol.2016.07.002 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DW8UH UT WOS:000383930500102 PM 27400906 ER PT J AU Morefield, PE LeDuc, SD Clark, CM Iovanna, R AF Morefield, Philip E. LeDuc, Stephen D. Clark, Christopher M. Iovanna, Richard TI Grasslands, wetlands, and agriculture: the fate of land expiring from the Conservation Reserve Program in the Midwestern United States SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE Conservation Reserve Program; land use change; corn; soy; grasslands; wetlands; agriculture AB The Conservation Reserve Program (CRP) is the largest agricultural land-retirement program in the United States, providing many environmental benefits, including wildlife habitat and improved air, water, and soil quality. Since 2007, however, CRP area has declined by over 25% nationally with much of this land returning to agriculture. Despite this trend, it is unclear what types of CRP land are being converted, to what crops, and where. All of these specific factors greatly affect environmental impacts. To answer these questions, we quantified shifts in expiring CRP parcels to five major crop-types (corn, soy, winter and spring wheat, and sorghum) in a 12-state, Midwestern region of the United States using a US Department of Agriculture (USDA), field-level CRP database and USDA's Cropland Data Layer. For the years 2010 through 2013, we estimate almost 30%, or more than 530 000 ha, of expiring CRP land returned to the production of these five crops in our study area, with soy and corn accounting for the vast majority of these shifts. Grasslands were the largest type of CRP land converted (360 000 ha), followed by specifically designated wildlife habitat (76 000 ha), and wetland areas (53 000 ha). These wetland areas were not just wetlands themselves, but also a mix of land covers enhancing or protecting wetland ecosystem services (e.g., wetland buffers). Areas in the Dakotas, Nebraska, and southern Iowa were hotspots of change, with the highest areas of CRP land moving back to agriculture. By contrast, we estimate only a small amount (similar to 3%) of the expiring land shifted into similar, non-CRP land-retirement or easement programs. Reconciling needs for food, feed, fuel, and healthy ecosystems is an immense challenge for farmers, conservationists, and state and federal agencies. Reduced enrollment and the turnover of CRP land from conservation to agriculture raises questions about sustaining ecosystem services in this region. C1 [Morefield, Philip E.; LeDuc, Stephen D.; Clark, Christopher M.] US EPA, Natl Ctr Environm Assessment, 1200 Penn Ave NW,ORD NCEA 8623-P, Washington, DC 20460 USA. [Iovanna, Richard] USDA, Farm Serv Agcy, POB 2415, Washington, DC 20250 USA. RP LeDuc, SD (reprint author), US EPA, Natl Ctr Environm Assessment, 1200 Penn Ave NW,ORD NCEA 8623-P, Washington, DC 20460 USA. EM leduc.stephen@epa.gov NR 27 TC 1 Z9 1 U1 21 U2 21 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD SEP PY 2016 VL 11 IS 9 AR 094005 DI 10.1088/1748-9326/11/9/094005 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DX0NQ UT WOS:000384060200001 ER PT J AU Levine, AD Yang, YJ Goodrich, JA AF Levine, Audrey D. Yang, Y. Jeffrey Goodrich, James A. TI Enhancing climate adaptation capacity for drinking water treatment facilities SO JOURNAL OF WATER AND CLIMATE CHANGE LA English DT Article DE climate adaptation; coagulation; conventional treatment; resilience; surface water; treatment system models ID DISSOLVED ORGANIC-CARBON; NITROSAMINE FORMATION; CHEMICAL SPILL; COAGULATION; IMPACTS; QUALITY; REMOVAL; PERFORMANCE; RESILIENCE; PRECURSORS AB Conventional water treatment processes (e.g., coagulation, flocculation, sedimentation, and filtration) are widely used for producing drinking water from surface water sources. Transient, gradual, or abrupt changes in source water quality that could compromise treatment effectiveness can be triggered by climate and related meteorological events, accidental or intentional contamination, security breaches, or other disruptions. However, the design principles that underpin the majority of existing conventional treatment systems predate climate adaptation considerations. This paper considers the adaptation capacity of conventional water treatment systems. A modeling framework is used to illustrate climate adaptation mechanisms that could enable conventional treatment systems to accommodate water quality impairments. Treatment system resiliency is explored in response to generic climate-relevant water quality perturbations such as extreme temperature variations and changes in the quantity and characteristics of solids, particles, and organic constituents. Promising adaptation options include modifying chemical parameters (e.g., types of chemicals, dosages, sequence of chemical addition, mixing intensity and duration), filter operations, and microbiological augmentation of existing physical/chemical treatment systems. The capacity reserve concept provides an organizing principle that could be useful for prioritizing climate adaptation strategies such as major or minor treatment/infrastructure modifications, system-wide upgrades such as off-line storage, operational changes in distribution systems, or the use of supplemental water sources including reclaimed or recycled water. C1 [Levine, Audrey D.] Natl Sci Fdn, Arlington, VA 22230 USA. [Levine, Audrey D.] Flinders Univ S Australia, Adelaide, SA, Australia. [Yang, Y. Jeffrey] US EPA, Off Res & Dev, Natl Risk Management Res Ctr, Cincinnati, OH 45268 USA. [Goodrich, James A.] US EPA, Off Res & Dev, Natl Homeland Secur Res Ctr, Cincinnati, OH 45268 USA. RP Levine, AD (reprint author), Natl Sci Fdn, Arlington, VA 22230 USA.; Levine, AD (reprint author), Flinders Univ S Australia, Adelaide, SA, Australia. EM 1audrey.levine@gmail.com FU US Environmental Protection Agency (EPA) Water Resources Adaptation Program; US National Science Foundation (NSF) Independent Research and Development Program FX This research was supported through the US Environmental Protection Agency (EPA) Water Resources Adaptation Program and the US National Science Foundation (NSF) Independent Research and Development Program. The authors are solely responsible for the content and writing of this paper, and any opinions, findings, conclusions, or recommendations are those of the authors and do not necessarily reflect the views or policies of the US EPA or the NSF. NR 56 TC 0 Z9 0 U1 8 U2 8 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 2040-2244 J9 J WATER CLIM CHANGE JI J. Water Clim. Chang. PD SEP PY 2016 VL 7 IS 3 BP 485 EP 497 DI 10.2166/wcc.2016.011 PG 13 WC Water Resources SC Water Resources GA DW9OA UT WOS:000383989000003 ER PT J AU Tong, STY Yang, H Chen, HY Yang, JY AF Tong, Susanna T. Y. Yang, Heng Chen, Heyin Yang, Jeffrey Y. TI Hydrologic impacts of climate change and urbanization in the Las Vegas Wash Watershed, Nevada SO JOURNAL OF WATER AND CLIMATE CHANGE LA English DT Article DE cell-based watershed modeling; climate change; hydrology; land-use change; rainfall-runoff simulation ID LAND-USE CHANGE; CONTERMINOUS UNITED-STATES; BALANCE MODEL; RUNOFF; STREAMFLOW; DYNAMICS; COVER; COMPLETION; PREDICTION; MANAGEMENT AB A cell-based model for the Las Vegas Wash Watershed in Clark County, Nevada, USA, was developed by combining the Thornthwaite water balance model and the Soil Conservation Survey's Curve Number method with pixel-based computing technology. After the model was validated, it was used to predict the 2030 and 2050 hydrologic conditions under future scenarios of climate and land-use changes. The future climate projections were based on the Intergovernmental Panel on Climate Change (IPCC) B1 climate scenario, and the land-use scenarios were derived from a CA-Markov land-use model. Results indicate that under these hypothetical conditions, the future surface runoff in the watershed will significantly decrease in winters but increase in summers. Climate change will be the primary controlling factor over runoff. Urban development is projected to increase runoff and may contribute 1.1-18.7% of the changes. This finding may be useful in devising future urban development plans and water management policies. C1 [Tong, Susanna T. Y.; Yang, Heng; Chen, Heyin] Univ Cincinnati, Dept Geog, Cincinnati, OH 45220 USA. [Yang, Jeffrey Y.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. RP Tong, STY (reprint author), Univ Cincinnati, Dept Geog, Cincinnati, OH 45220 USA. EM susanna.tong@uc.edu FU US Environmental Protection Agency FX This research was partially funded by the US Environmental Protection Agency. The authors are grateful to the agency for the financial support. NR 76 TC 0 Z9 0 U1 12 U2 12 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 2040-2244 J9 J WATER CLIM CHANGE JI J. Water Clim. Chang. PD SEP PY 2016 VL 7 IS 3 BP 598 EP 620 DI 10.2166/wcc.2016.038 PG 23 WC Water Resources SC Water Resources GA DW9OA UT WOS:000383989000010 ER PT J AU Stapanian, MA Rodriguez, K Lewis, TE Blume, L Palmer, CJ Walters, L Schofield, J Amos, MM Bucher, A AF Stapanian, Martin A. Rodriguez, Karen Lewis, Timothy E. Blume, Louis Palmer, Craig J. Walters, Lynn Schofield, Judith Amos, Molly M. Bucher, Adam TI Announcementguidance document for acquiring reliable data in ecological restoration projects SO RESTORATION ECOLOGY LA English DT Article DE Great Lakes Restoration Initiative; guidance document; QA; QC AB The Laurentian Great Lakes are undergoing intensive ecological restoration in Canada and the United States. In the United States, an interagency committee was formed to facilitate implementation of quality practices for federally funded restoration projects in the Great Lakes basin. The Committee's responsibilities include developing a guidance document that will provide a common approach to the application of quality assurance and quality control (QA/QC) practices for restoration projects. The document will serve as a how-to guide for ensuring data quality during each aspect of ecological restoration projects. In addition, the document will provide suggestions on linking QA/QC data with the routine project data and hints on creating detailed supporting documentation. Finally, the document will advocate integrating all components of the project, including QA/QC applications, into an overarching decision-support framework. The guidance document is expected to be released by the U.S. EPA Great Lakes National Program Office in 2017. C1 [Stapanian, Martin A.] US Geol Survey, 6100 Columbus Ave, Sandusky, OH 44870 USA. [Rodriguez, Karen; Blume, Louis] US EPA, Great Lakes Natl Program Off, 77 West Jackson,G-17J, Chicago, IL 60604 USA. [Lewis, Timothy E.] US Army Engineer Res & Dev Ctr, 3909 Halls Ferry Rd, Vicksburg, MS 39180 USA. [Palmer, Craig J.; Walters, Lynn; Schofield, Judith; Amos, Molly M.; Bucher, Adam] CSC Govt Solut LLC, 6361 Walker Lane, Alexandria, VA 22310 USA. RP Rodriguez, K (reprint author), US EPA, Great Lakes Natl Program Off, 77 West Jackson,G-17J, Chicago, IL 60604 USA. EM rodriguez.karen@epa.gov FU U.S. Environmental Protection Agency [EP-C-12-008] FX Use of trade, product, or firm names does not imply endorsement by the U.S. EPA, U.S. Geological Survey, U.S. Army, or any other agency of the United States government. This article is Contribution 2028 of the U.S. Geological Survey Great Lakes Science Center. Some of the information in this document has been funded by the U.S. Environmental Protection Agency under contract number EP-C-12-008 to CSC. Although some of the research described in this article has been funded by the U.S. Environmental Protection Agency, it has not been subjected to Agency review. Any opinions expressed in this publication are those of the author(s) and do not, necessarily, reflect the official positions and policies of the U.S. EPA. Opinions, interpretations, conclusions, and recommendations are those of the authors and are not necessarily endorsed by the U.S. Army. NR 10 TC 0 Z9 0 U1 11 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD SEP PY 2016 VL 24 IS 5 BP 570 EP 572 DI 10.1111/rec.12367 PG 3 WC Ecology SC Environmental Sciences & Ecology GA DW9UW UT WOS:000384009200001 ER PT J AU Lieber, M AF Lieber, Michael TI The Golden Ratio (1.62) as a dimensionless biological constant SO SOUTH AFRICAN JOURNAL OF SCIENCE LA English DT Editorial Material C1 [Lieber, Michael] US EPA, Div 9, San Francisco, CA USA. EM michaellieber@juno.com NR 3 TC 0 Z9 0 U1 2 U2 2 PU ACAD SCIENCE SOUTH AFRICA A S S AF PI LYNWOOD RIDGE PA PO BOX 72135, LYNWOOD RIDGE 0040, SOUTH AFRICA SN 0038-2353 EI 1996-7489 J9 S AFR J SCI JI S. Afr. J. Sci. PD SEP-OCT PY 2016 VL 112 IS 9-10 BP 2 EP 2 DI 10.17159/sajs.2016/a0172 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DX4DS UT WOS:000384331800002 ER PT J AU Jun, YS Kim, D Neil, CW AF Jun, Young-Shin Kim, Doyoon Neil, Chelsea W. TI Heterogeneous Nucleation and Growth of Nanoparticles at Environmental Interfaces SO ACCOUNTS OF CHEMICAL RESEARCH LA English DT Review ID MANAGED AQUIFER RECHARGE; IN-SITU OBSERVATIONS; IRON(III) (HYDR)OXIDES; ARSENIC MOBILIZATION; CALCIUM-CARBONATE; QUARTZ; PRECIPITATION; DISSOLUTION; ENERGIES; IMPACTS AB Mineral nucleation is a phase transformation of aqueous components to solids with an accompanying creation of new surfaces. In this evolutional, yet elusive, process, nuclei often form at environmental interfaces, which provide remarkably reactive sites for heterogeneous nucleation and growth. Naturally occurring nucleation processes significantly contribute to the biogeochemical cycles of important components in the Earths crust, such as iron and manganese oxide minerals and calcium carbonate. However, in recent decades, these cycles have been significantly altered by anthropogenic activities, which affect the aqueous chemistry and equilibrium of both surface and subsurface systems. These alterations can trigger the dissolution of existing minerals and formation of new nanoparticles (i.e., nucleation and growth) and consequently change the porosity and permeability of geomedia in subsurface environments. Newly formed nanoparticles can also actively interact with components in natural and engineered aquatic systems, including those posing a significant hazard such as arsenic. These interactions can bilaterally influence the fate and transport of both newly formed nanoparticles and aqueous components. Due to their importance in natural and engineered processes, heterogeneous nucleation at environmental interfaces has started to receive more attention. However, a lack of time-resolved in situ analyses makes the evaluation of heterogeneous nucleation challenging because the physicochemical properties of both the nuclei and surfaces significantly and dynamically change with time and aqueous chemistry. This Account reviews our in situ kinetic studies of the heterogeneous nucleation and growth behaviors of iron(III) (hydr)oxide, calcium carbonate, and manganese (hydr)oxide minerals in aqueous systems. In particular, we utilized simultaneous small-angle and grazing incidence small-angle X-ray scattering (SAXS/GISAXS) to investigate in situ and in real-time the effects of water chemistry and substrate identity on heterogeneously and homogeneously formed nanoscale precipitate size dimensions and total particle volume. Using this technique, we also provided a new platform for quantitatively comparing between heterogeneous and homogeneous nucleation and growth of nanoparticles and obtaining undiscovered interfacial energies between nuclei and surfaces. In addition, nanoscale surface characterization tools, such as in situ atomic force microscopy (AFM), were utilized to support and complement our findings. With these powerful nanoscale tools, we systematically evaluated the influences of environmentally abundant (oxy)anions and cations and the properties of environmental surfaces, such as surface charge and hydrophobicity. The findings, significantly enhanced by in situ observations, can lead to a more accurate prediction of the behaviors of nanoparticles in the environment and enable better control of the physicochemical properties of nanoparticles in engineered systems, such as catalytic reactions and energy storage. C1 [Jun, Young-Shin; Kim, Doyoon; Neil, Chelsea W.] Washington Univ, Dept Energy Environm & Chem Engn, St Louis, MO 63130 USA. [Neil, Chelsea W.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. RP Jun, YS (reprint author), Washington Univ, One Brookings Dr,Campus Box 1180, St Louis, MO 63130 USA. EM ysjun@seas.wustl.edu FU National Science Foundation [EAR-1424927, CHE-1214090]; Center for Nanoscale Control of Geologic CO2, an Energy Frontier Research Center - U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-05CH11231]; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-06CH11357] FX We are grateful for support received from National Science Foundation (Grants EAR-1424927 and CHE-1214090). This is also partially supported by the Center for Nanoscale Control of Geologic CO2, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Award Number DE-AC02-05CH11231. We thank Environmental NanoChemistry Group members for valuable discussion and review. Use of the Advanced Photon Source (Sector 12 ID-B for SAXS/GISAXS) at Argonne National Laboratory was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357. NR 38 TC 2 Z9 2 U1 33 U2 33 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0001-4842 EI 1520-4898 J9 ACCOUNTS CHEM RES JI Accounts Chem. Res. PD SEP PY 2016 VL 49 IS 9 BP 1681 EP 1690 DI 10.1021/acs.accounts.6b00208 PG 10 WC Chemistry, Multidisciplinary SC Chemistry GA DX0GA UT WOS:000384038600010 PM 27513685 ER PT J AU Xing, J Mathur, R Pleim, J Hogrefe, C Wang, JD Gan, CM Sarwar, G Wong, DC McKeen, S AF Xing, Jia Mathur, Rohit Pleim, Jonathan Hogrefe, Christian Wang, Jiandong Gan, Chuen-Meei Sarwar, Golam Wong, David C. McKeen, Stuart TI Representing the effects of stratosphere-troposphere exchange on 3-D O-3 distributions in chemistry transport models using a potential vorticity-based parameterization SO ATMOSPHERIC CHEMISTRY AND PHYSICS LA English DT Article ID NORTHERN-HEMISPHERE; TROPOPAUSE FOLD; CUTOFF LOW; OZONE; INTRUSIONS; IMPACT; EAST AB Downward transport of ozone (O-3) from the stratosphere can be a significant contributor to tropospheric O-3 background levels. However, this process often is not well represented in current regional models. In this study, we develop a seasonally and spatially varying potential vorticity (PV)-based function to parameterize upper tropospheric and/or lower stratospheric (UTLS) O-3 in a chemistry transport model. This dynamic O-3-PV function is developed based on 21-year ozonesonde records from World Ozone and Ultraviolet Radiation Data Centre (WOUDC) with corresponding PV values from a 21-year Weather Research and Forecasting (WRF) simulation across the Northern Hemisphere from 1990 to 2010. The result suggests strong spatial and seasonal variations of O-3 / PV ratios which exhibits large values in the upper layers and in high-latitude regions, with highest values in spring and the lowest values in autumn over an annual cycle. The newly developed O-3 / PV function was then applied in the Community Multiscale Air Quality (CMAQ) model for an annual simulation of the year 2006. The simulated UTLS O-3 agrees much better with observations in both magnitude and seasonality after the implementation of the new parameterization. Considerable impacts on surface O-3 model performance were found in the comparison with observations from three observational networks, i.e., EMEP, CASTNET and WDCGG. With the new parameterization, the negative bias in spring is reduced from -20 to -15% in the reference case to -9 to -1 %, while the positive bias in autumn is increased from 1 to 15% in the ref-erence case to 5 to 22 %. Therefore, the downward transport of O-3 from upper layers has large impacts on surface concentration and needs to be properly represented in regional models. C1 [Xing, Jia; Mathur, Rohit; Pleim, Jonathan; Hogrefe, Christian; Wang, Jiandong; Gan, Chuen-Meei; Sarwar, Golam; Wong, David C.] US EPA, Res Triangle Pk, NC 27711 USA. [Xing, Jia; Wang, Jiandong] Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China. [McKeen, Stuart] Univ Colorado, Cooperat Inst Res Environm Sci, Boulder, CO USA. RP Mathur, R (reprint author), US EPA, Res Triangle Pk, NC 27711 USA. EM mathur.rohit@epa.gov RI Pleim, Jonathan Pleim/C-1331-2017 OI Pleim, Jonathan Pleim/0000-0001-6190-6082 FU National Research Council FX The authors gratefully acknowledge the free availability and use of data sets from the WOUDC, WDCGG, CASTNET, EMEP monitoring networks. During the conduct of this work, J. Xing and C.-M. Gan held National Research Council post-doctoral fellowships. J. Wang was a visiting student at the U.S. Environmental Protection Agency. NR 24 TC 0 Z9 0 U1 9 U2 9 PU COPERNICUS GESELLSCHAFT MBH PI GOTTINGEN PA BAHNHOFSALLEE 1E, GOTTINGEN, 37081, GERMANY SN 1680-7316 EI 1680-7324 J9 ATMOS CHEM PHYS JI Atmos. Chem. Phys. PD SEP 1 PY 2016 VL 16 IS 17 BP 10865 EP 10877 DI 10.5194/acp-16-10865-2016 PG 13 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DW6FC UT WOS:000383744000001 ER PT J AU Hassan, I Kumar, AM Park, HR Lash, LH Loch-Caruso, R AF Hassan, Iman Kumar, Anjana M. Park, Hae-Ryung Lash, Lawrence H. Loch-Caruso, Rita TI Reactive Oxygen Stimulation of Interleukin-6 Release in the Human Trophoblast Cell Line HTR-8/SVneo by the Trichlorethylene Metabolite S-(1,2-Dichloro)-L-Cysteine SO BIOLOGY OF REPRODUCTION LA English DT Article DE cytokines; DCVC; HTR-8/SVneo cells; human placental cells; oxidative stress; reactive oxygen species; S-(1,2-dichlorovinyl)-L-cysteine; trichloroethylene; trophoblasts ID PROXIMAL TUBULAR CELLS; HUMAN EXTRAVILLOUS TROPHOBLAST; LYASE-MEDIATED TOXICITY; RAT-KIDNEY CELLS; OXIDATIVE STRESS; IN-VITRO; LIPID-PEROXIDATION; PRIMARY CULTURES; 1ST TRIMESTER; SCIENTIFIC ISSUES AB Trichloroethylene (TCE) is a common environmental pollutant associated with adverse reproductive outcomes in humans. TCE intoxication occurs primarily through its biotransformation to bioactive metabolites, including S-(1,2-dichlorovinyl)-L-cysteine (DCVC). TCE induces oxidative stress and inflammation in the liver and kidney. Although the placenta is capable of xenobiotic metabolism and oxidative stress and inflammation in placenta have been associated with adverse pregnancy outcomes, TCE toxicity in the placenta remains poorly understood. We determined the effects of DCVC by using the human extravillous trophoblast cell line HTR-8/SVneo. Exposure to 10 and 20 mu M DCVC for 10 h increased reactive oxygen species (ROS) as measured by carboxydichlorofluorescein fluorescence. Moreover, 10 and 20 mu M DCVC increased mRNA expression and release of interleukin-6 (IL-6) after 24-h exposure, and these responses were inhibited by the cysteine conjugate beta-lyase inhibitor aminooxyacetic acid and by treatments with antioxidants (alpha-tocopherol and deferoxamine), suggesting that DCVC-stimulated IL-6 release in HTR-8/SVneo cells is dependent on beta-lyase metabolic activation and increased generation of ROS. HTR-8/SVneo cells exhibited decreased mitochondrial membrane potential at 5, 10, and 20 mu M DCVC at 5, 10, and 24 h, showing that DCVC induces mitochondrial dysfunction in HTR-8/Svneo cells. The present study demon-strates that DCVC stimulated ROS generation in the human placental cell line HTR-8/SVneo and provides new evidence of mechanistic linkage between DCVC-stimulated ROS and increase in proinflammatory cytokine IL-6. Because abnormal activation of cytokines can disrupt trophoblast functions necessary for placental development and successful pregnancy, follow-up investigations relating these findings to physiologic outcomes are warranted. C1 [Hassan, Iman; Kumar, Anjana M.; Park, Hae-Ryung; Loch-Caruso, Rita] Univ Michigan, Dept Environm Hlth Sci, 1415 Washington Hts, Ann Arbor, MI 48109 USA. [Lash, Lawrence H.] Wayne State Univ, Sch Med, Dept Pharmacol, Detroit, MI 48201 USA. [Hassan, Iman] US EPA, Tox Assessment Div, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Park, Hae-Ryung] Harvard TH Chan Sch Publ Hlth, Program Mol & Integrat Physiol Sci, Dept Environm Hlth, Boston, MA 02115 USA. [Park, Hae-Ryung] Harvard TH Chan Sch Publ Hlth, Program Mol & Integrat Physiol Sci, Dept Genet & Complex Dis, Boston, MA 02115 USA. RP Loch-Caruso, R (reprint author), Univ Michigan, Dept Environm Hlth Sci, 1415 Washington Hts, Ann Arbor, MI 48109 USA. EM rlc@umich.edu FU National Institutes of Health (NIH)/National Institute of Environmental Health Sciences grant [P42 ES017198]; NIH Ruth L. Kirschstein NRSA Institutional grant [T32 ES007062]; NIH Ruth L. Kirschstein NRSA Individual grant [F31 ES021238]; Michigan Center for Lifestage Environmental Exposures and Disease grant [P30 ES017885] FX Supported by National Institutes of Health (NIH)/National Institute of Environmental Health Sciences grant P42 ES017198 to R.L.-C.; NIH Ruth L. Kirschstein NRSA Institutional grant T32 ES007062 and Individual grant F31 ES021238 for predoctoral fellowships to I.H.; and Michigan Center for Lifestage Environmental Exposures and Disease grant P30 ES017885. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. Portions of this work were presented at the 25th Annual Meeting of the Superfund Research Program, October 21-24, 2012, Raleigh, NC, and the 27th Annual Meeting of the Superfund Research Program, November 12-14, 2014, University of California Berkeley, CA. NR 58 TC 0 Z9 0 U1 4 U2 4 PU SOC STUDY REPRODUCTION PI MADISON PA 1691 MONROE ST,SUITE # 3, MADISON, WI 53711-2021 USA SN 0006-3363 EI 1529-7268 J9 BIOL REPROD JI Biol. Reprod. PD SEP 1 PY 2016 VL 95 IS 3 AR 66 DI 10.1095/biolreprod.116.139261 PG 11 WC Reproductive Biology SC Reproductive Biology GA DW8PE UT WOS:000383917200019 ER PT J AU Pinto, CL Mansouri, K Judson, R Browne, P AF Pinto, Caroline L. Mansouri, Kamel Judson, Richard Browne, Patience TI Prediction of Estrogenic Bioactivity of Environmental Chemical Metabolites SO CHEMICAL RESEARCH IN TOXICOLOGY LA English DT Article ID RAT-LIVER MICROSOMES; YEAST 2-HYBRID ASSAY; REPORTER GENE ASSAY; POLYCYCLIC AROMATIC-HYDROCARBONS; ENDOCRINE-DISRUPTING ACTIVITY; ANTI-ANDROGENIC ACTIVITIES; FOOD CONTACT PLASTICS; IN-VITRO METABOLISM; RECEPTOR-ALPHA; CELL-LINES AB The US Environmental Protection Agency's (EPA) Endocrine Disruptor Screening Program (EDSP) is using in vitro data generated from ToxCast/Tox21 high-throughput screening assays to assess the endocrine activity of environmental chemicals. Considering that in vitro assays may have limited metabolic capacity, inactive chemicals that are biotransformed into metabolites with endocrine bioactivity may be missed for further screening and testing. Therefore, there is a value in developing novel approaches to account for metabolism and endocrine activity of both parent chemicals and their associated metabolites. We used commercially available software to predict metabolites of SO parent compounds, out of which 38 chemicals are known to have estrogenic. metabolites, and 12 compounds and their metabolites are negative for estrogenic activity. Three ER QSAR models were used to determine potential estrogen bioactivity of the parent compounds and predicted metabolites, the outputs of the models were averaged, and the chemicals were then ranked based on the total estrogenicity of the parent chemical and metabolites. The metabolite prediction software correctly identified known estrogenic metabolites for 26 out of 27 parent chemicals with associated metabolite data, and 39 out of 46 estrogenic metabolites were predicted as potential biotransformation products derived from the parent chemical. The QSAR models estimated stronger estrogenic activity for the majority of the known estrogenic metabolites compared to their parent chemicals. Finally, the three models identified a similar set of parent compounds as top ranked chemicals based on the. estrogenicity of putative metabolites. This proposed in silico approach is an inexpensive and rapid strategy for the detection of chemicals with estrogenic metabolites and may reduce potential false negative results from in vitro assays. C1 [Pinto, Caroline L.; Browne, Patience] US EPA, Off Chem Safety & Pollut Prevent, 1200 Penn Ave NW, Washington, DC 20460 USA. [Pinto, Caroline L.; Mansouri, Kamel] Oak Ridge Inst Sci & Educ, MC 100-44,POB 117, Oak Ridge, TN 37831 USA. [Mansouri, Kamel; Judson, Richard] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Pinto, CL (reprint author), US EPA, Off Chem Safety & Pollut Prevent, 1200 Penn Ave NW, Washington, DC 20460 USA.; Pinto, CL (reprint author), Oak Ridge Inst Sci & Educ, MC 100-44,POB 117, Oak Ridge, TN 37831 USA. EM pinto.caroline@epa.gov OI Mansouri, Kamel/0000-0002-6426-8036 NR 71 TC 1 Z9 1 U1 17 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0893-228X EI 1520-5010 J9 CHEM RES TOXICOL JI Chem. Res. Toxicol. PD SEP PY 2016 VL 29 IS 9 BP 1410 EP 1427 DI 10.1021/acs.chemrestox.6b00079 PG 18 WC Chemistry, Medicinal; Chemistry, Multidisciplinary; Toxicology SC Pharmacology & Pharmacy; Chemistry; Toxicology GA DW6CF UT WOS:000383733300006 PM 27509301 ER PT J AU Cheng, QY Teng, Q Marchitti, SA Dillingham, CM Kenneke, JF AF Cheng, Qianyi Teng, Quincy Marchitti, Satori A. Dillingham, Caleb M. Kenneke, John F. TI An Integrated Experimental and Computational Approach for Characterizing the Kinetics and Mechanism of TriadimefonRacemization SO CHIRALITY LA English DT Article DE chirality; conazoles; enantiomerization; environmental chemicals; pesticides; risk assessment; stereochemistry ID RACEMIZATION; TRIADIMEFON; DENSITY; DRUGS; ENANTIOMERIZATION; METABOLISM; PRODUCTS; EXCHANGE; SURFACE; WATER AB Enantiomers of chiral molecules commonly exhibit differing pharmacokinetics and toxicities, which can introduce significant uncertainty when evaluating biological and environmental fates and potential risks to humans and the environment. However, racemization (the irreversible transformation of one enantiomer into the racemic mixture) and enantiomerization (the reversible conversion of one enantiomer into the other) are poorly understood. To better understand these processes, we investigated the chiral fungicide, triadimefon, which undergoes racemization in soils, water, and organic solvents. Nuclear magnetic resonance (NMR) and gas chromatography / mass spectrometry (GC/MS) techniques were used to measure the rates of enantiomerization and racemization, deuterium isotope effects, and activation energies for triadimefon in H2O and D2O. From these results we were able to determine that: 1) the alpha-carbonyl carbon of triadimefon is the reaction site; 2) cleavage of the C-H (C-D) bond is the rate-determining step; 3) the reaction is base-catalyzed; and 4) the reaction likely involves a symmetrical intermediate. The B3LYP/6-311+G** level of theory was used to compute optimized geometries, harmonic vibrational frequencies, nature population analysis, and intrinsic reaction coordinates for triadimefon in water and three racemization pathways were hypothesized. This work provides an initial step in developing predictive, structure-based models that are needed to identify compounds of concern that may undergo racemization. Chirality 28:633-641, 2016. (c) 2016 Wiley Periodicals, Inc. C1 [Cheng, Qianyi; Teng, Quincy; Marchitti, Satori A.; Dillingham, Caleb M.; Kenneke, John F.] US EPA, Natl Exposure Res Lab, Athens, GA USA. RP Kenneke, JF (reprint author), US EPA, 960 Coll Stn Rd, Athens, GA 30605 USA. EM kenneke.john@epa.gov FU U.S. Environmental Protection Agency (U.S. EPA); U.S. Department of Energy [DW8992298301]; U.S. EPA [DW8992298301]; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231] FX This research received funding from the U.S. Environmental Protection Agency (U.S. EPA) and by an appointment to the Postdoctoral Research Program at the Ecosystems Research Division, administered by the Oak Ridge Institute for Science and Education through Interagency Agreement No. (DW8992298301) between the U.S. Department of Energy and the U.S. EPA. This research also used resources of the National Energy Research Scientific Computing Center, which is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The views expressed in this article are those of the author[s] and do not necessarily represent the views or policies of the U.S. EPA. The authors declare they have no competing financial interests. We thank Professor Henry F. Schaefer, III, Dr. Jiande Gu, Dr. Keigo Ito, and Dr. Judy Wu for insightful discussions and technical expertise. Current address for Q. Cheng: School of Computational Sciences, Korea Institute for Advanced Study, 85 Hoegiro Dongdaemun-gu, Seoul, 02455, South Korea. NR 38 TC 0 Z9 0 U1 10 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0899-0042 EI 1520-636X J9 CHIRALITY JI Chirality PD SEP PY 2016 VL 28 IS 9 BP 633 EP 641 DI 10.1002/chir.22622 PG 9 WC Chemistry, Medicinal; Chemistry, Analytical; Chemistry, Organic; Pharmacology & Pharmacy SC Pharmacology & Pharmacy; Chemistry GA DW7TU UT WOS:000383855800003 PM 27479933 ER PT J AU Awkerman, JA Hemmer, B Almario, A Lilavois, C Barron, MG Raimondo, S AF Awkerman, Jill A. Hemmer, Becky Almario, Alex Lilavois, Crystal Barron, Mace G. Raimondo, Sandy TI Spatially explicit assessment of estuarine fish after Deepwater Horizon oil spill: trade-off in complexity and parsimony SO ECOLOGICAL APPLICATIONS LA English DT Article DE sheepshead minnow; Louisiana; Barataria Bay; population model; PAH; Deepwater Horizon; Cyprinodon variegatus ID MINNOW CYPRINODON-VARIEGATUS; SHEEPSHEAD MINNOW; POPULATION-LEVEL; MULTIGENERATIONAL EXPOSURE; WILDLIFE POPULATIONS; MULTIPLE STRESSORS; R-PACKAGE; LOUISIANA; RESPONSES; TOXICITY AB Evaluating long-term contaminant effects on wildlife populations depends on spatial information about habitat quality, heterogeneity in contaminant exposure, and sensitivities and distributions of species integrated into a systems modeling approach. Rarely is this information readily available, making it difficult to determine the applicability of realistic models to quantify population-level risks. To evaluate the trade-offs between data demands and increased specificity of spatially explicit models for population-level risk assessments, we developed a model for a standard toxicity test species, the sheepshead minnow (Cyprinodon variegatus), exposed to oil contamination following the Deepwater Horizon oil spill and compared the output with various levels of model complexity to a standard risk quotient approach. The model uses habitat and fish occupancy data collected over five sampling periods throughout 2008-2010 in Pensacola and Choctawhatchee Bays, Florida, USA, to predict species distribution, field-collected and publically available data on oil distribution and concentration, and chronic toxicity data from laboratory assays applied to a matrix population model. The habitat suitability model established distribution of fish within Barataria Bay, Louisiana, USA, and the population model projected the dynamics of the species in the study area over a 5-yr period (October 2009-September 2014). Vital rates were modified according to estimated contaminant concentrations to simulate oil exposure effects. To evaluate the differences in levels of model complexity, simulations varied from temporally and spatially explicit, including seasonal variation and location-specific oiling, to simple interpretations of a risk quotient derived for the study area. The results of this study indicate that species distribution, as well as spatially and temporally variable contaminant concentrations, can provide a more ecologically relevant evaluation of species recovery from catastrophic environmental impacts but might not be cost-effective or efficient for rapid assessment needs. C1 [Awkerman, Jill A.; Hemmer, Becky; Almario, Alex; Lilavois, Crystal; Barron, Mace G.; Raimondo, Sandy] US EPA, Gulf Ecol Div, 1 Sabine Isl Dr, Gulf Breeze, FL 32561 USA. RP Awkerman, JA (reprint author), US EPA, Gulf Ecol Div, 1 Sabine Isl Dr, Gulf Breeze, FL 32561 USA. EM Awkerman.Jill@epa.gov NR 51 TC 0 Z9 0 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD SEP PY 2016 VL 26 IS 6 BP 1708 EP 1720 DI 10.1890/15-1410.1 PG 13 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DW0UJ UT WOS:000383358000009 PM 27755711 ER PT J AU Tsang, YP Infante, DM Stewart, J Wang, LZ Tingly, RW Thornbrugh, D Cooper, AR Daniel, WM AF Tsang, Yin-Phan Infante, Dana M. Stewart, Jana Wang, Lizhu Tingly, Ralph W., III Thornbrugh, Darren Cooper, Arthur R. Daniel, Wesley M. TI StreamThermal: A Software Package for Calculating Thermal Metrics from Stream Temperature Data SO FISHERIES LA English DT Article ID FISH ASSEMBLAGES; REGIME AB Improving quality and better availability of continuous stream temperature data allow natural resource managers, particularly in fisheries, to understand associations between different characteristics of stream thermal regimes and stream fishes. However, there is no convenient tool to efficiently characterize multiple metrics reflecting stream thermal regimes with the increasing amount of data from continuously recording data loggers. This article describes a software program packaged as a library in R to facilitate this process. With this freely available package, users will be able to quickly summarize metrics that describe five categories of stream thermal regimes: magnitude, variability, frequency, timing, and rate of change. The installation and usage instruction of this package, the definition of calculated thermal metrics, as well as the output format from the package are described, along with an application showing the utility for multiple metrics. We believe that this package can be widely utilized by interested stakeholders and can greatly assist future fisheries studies. Mejorar la calidad y disponibilidad de datos continuos de temperatura en los rios permite a manejadores de recursos naturales, particularmente en pesquerias, entender la relacion entre diferentes caracteristicas de regimenes termicos en los rios y los peces que los habitan. No obstante, no existe una herramienta conveniente para caracterizar de forma eficiente diferente metricas que reflejen regimenes termicos fluviales mediante la creciente cantidad de datos provenientes de dispositivos de registro continuo. En este articulo se describe un software programado en lenguaje R con el fin de facilitar dicho proceso. Con este software de acceso gratuito, los usuarios seran capaces de resumir rapidamente las metricas que describen cinco categorias de regimenes termicos en rios: magnitud, variabilidad, frecuencia, sincronizacion y tasa de cambio. Se describe la instalacion e instrucciones de uso, la definicion de las metricas utilizadas y el formato de salida de los archivos del programa; asi mismo se ofrece una aplicacion que muestra la utilidad de diferentes metricas. Creemos que este paquete puede ser ampliamente utilizado por usuarios interesados y puede ser de ayuda en un futuro para estudios de pesquerias. L'amelioration de la qualite et une meilleure disponibilite des donnees en continu de la temperature des cours d'eau permet aux gestionnaires de ressources naturelles, en particulier dans les pecheries, de comprendre les associations entre les differentes caracteristiques des regimes thermiques des cours d'eau et les poissons de ruisseaux. Cependant, il n'existe pas d'outil pratique pour caracteriser efficacement plusieurs parametres refletant les regimes thermiques des cours d'eau avec une quantite croissante de donnees provenant d'enregistreurs de donnees en continu. Cet article decrit un logiciel conditionne en package sous R pour faciliter ce processus. Avec ce package, disponible gratuitement, les utilisateurs seront en mesure de resumer rapidement les parametres decrivant cinq categories de regimes thermiques des cours d'eau: l'amplitude, la variabilite, la frequence, le temps, et le taux de changement. Les instructions d'installation et d'utilisation de ce package, la definition des parametres thermiques calcules, ainsi que le format de sortie du package sont decrits, et une application montre l'utilite de plusieurs parametres. Nous croyons que ce package peut etre largement utilise par les parties interessees et peut grandement aider les futures etudes sur les peches. C1 [Tsang, Yin-Phan; Infante, Dana M.; Tingly, Ralph W., III; Cooper, Arthur R.; Daniel, Wesley M.] Michigan State Univ, Dept Fisheries & Wildlife, 1405 S Harrison Rd,Suite 318, E Lansing, MI 48823 USA. [Stewart, Jana] US Geol Survey, Wisconsin Water Sci Ctr, Middleton, WI USA. [Wang, Lizhu] Int Joint Commiss, Great Lakes Reg Off, Windsor, ON, Canada. [Thornbrugh, Darren] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Thornbrugh, Darren] US EPA, Natl Hlth & Environm Effects Res Lab, Western Ecol Div, Corvallis, OR USA. [Tsang, Yin-Phan] Univ Hawaii Manoa, Dept Nat Resources & Environm Management, 1910 East West Rd,Sherman 243, Honolulu, HI 96822 USA. RP Tsang, YP (reprint author), Michigan State Univ, Dept Fisheries & Wildlife, 1405 S Harrison Rd,Suite 318, E Lansing, MI 48823 USA.; Tsang, YP (reprint author), Univ Hawaii Manoa, Dept Nat Resources & Environm Management, 1910 East West Rd,Sherman 243, Honolulu, HI 96822 USA. EM tsangy@hawaii.edu FU U.S. Geological Survey National Climate Change and Wildlife Science Center; U.S. Department of Interior Northeast Climate Science Center FX This product was developed with support from the U.S. Geological Survey National Climate Change and Wildlife Science Center and was also supported by the U.S. Department of Interior Northeast Climate Science Center. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 11 TC 0 Z9 0 U1 3 U2 3 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0363-2415 EI 1548-8446 J9 FISHERIES JI Fisheries PD SEP PY 2016 VL 41 IS 9 BP 548 EP 554 DI 10.1080/03632415.2016.1210517 PG 7 WC Fisheries SC Fisheries GA DW1NH UT WOS:000383409400013 ER PT J AU Schoen, LS Student, JJ Hoffman, JC Sierszen, ME Uzarski, DG AF Schoen, Lee S. Student, James J. Hoffman, Joel C. Sierszen, Michael E. Uzarski, Donald G. TI Reconstructing fish movements between coastal wetland and nearshore habitats of the Great Lakes SO LIMNOLOGY AND OCEANOGRAPHY LA English DT Article ID YELLOW PERCH; OTOLITH MICROCHEMISTRY; ONCORHYNCHUS-MYKISS; ELEMENTAL ANALYSIS; TRACE-ELEMENTS; FOOD WEBS; SUPERIOR; MICHIGAN; TEMPERATURE; SHORELINE AB The use of resources from multiple habitats has been shown to be important to the production of aquatic consumers. To quantify the support of Great Lakes coastal wetland (WL) and nearshore (NS) habitats to yellow perch, we used otolith microchemistry to trace movements between the habitats. WL and NS water and fish samples were collected from lakes Huron and Michigan for water and otolith trace element analysis. Recently deposited otolith-edge Sr : Ca and Ba : Ca from otoliths were strongly correlated with the chemistry of the water in which fish were caught. In general, Sr : Ca and Ba : Ca in otoliths were significantly greater for individuals collected from WL areas. Because of these observed chemical differences between WL and NS habitats, quadratic discriminant function analysis (QDFA) was used to classify individuals with high accuracy to the habitat from which they were collected. We then combined the predictive abilities of QDFA with the otolith chemistry transect data that represents an individuals' entire life, to classify habitat use through each fish's life. Our results suggest larval use of WL habitats as well as three life histories for adult yellow perch. These strategies include (1) fish utilizing WL once annually (2) WL residents (3) WL residence as juveniles followed by movement to nearshore as adults. This application represents a novel use of transect otolith microchemistry to reconstruct fish movements between freshwater environments across entire life spans at fine scales. These results suggest that regular movements of fish may facilitate the production of coastal fishes in the Great Lakes. C1 [Schoen, Lee S.; Uzarski, Donald G.] Cent Michigan Univ, Dept Biol, Inst Great Lakes Res, Mt Pleasant, MI 48859 USA. [Student, James J.] Cent Michigan Univ, Ctr Elemental & Isotop Anal, Coll Sci & Technol, Mt Pleasant, MI 48859 USA. [Hoffman, Joel C.; Sierszen, Michael E.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Midcontinent Ecol Div, Duluth, MN USA. RP Schoen, LS (reprint author), Cent Michigan Univ, Dept Biol, Inst Great Lakes Res, Mt Pleasant, MI 48859 USA. EM schoelee@gmail.com FU U.S. Environmental Protection Agency as part of the Great Lakes Restoration Initiative [EPA GLNPO-2010-H-3-984-758]; Garden Club of America Coastal Wetlands Award Selection Committee, Center for Coastal Resources Management at the Virginia Institute of Marine Science, College of William and Mary FX We thank David Clapp, Dave Fielder, Bill Wellenkamp and the Michigan Department of Natural Resources Fisheries Division for assisting in fish collections as well as Ryan Wheeler, Tom Langer, Bridget Ziola, Lindsey Adams and Kaley Genther for assisting in the collection and preparation of samples for analysis. We also thank Jonathon Launspach for his help creating maps. The research described in this article has been funded in part by the U.S. Environmental Protection Agency as part of the Great Lakes Restoration Initiative under proposal EPA GLNPO-2010-H-3-984-758 titled GLIC : Implementing Great Lakes Coastal Wetland Monitoring. The research was also supported by the Garden Club of America Coastal Wetlands Award Selection Committee, Center for Coastal Resources Management at the Virginia Institute of Marine Science, College of William and Mary. The views expressed in this article are those of the authors and do not necessarily reflect the views or policies of the Michigan DNR, DEQ or the U.S. EPA. This paper is Contribution Number 73 of the Central Michigan University Institute for Great Lakes Research. NR 48 TC 2 Z9 2 U1 19 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0024-3590 EI 1939-5590 J9 LIMNOL OCEANOGR JI Limnol. Oceanogr. PD SEP PY 2016 VL 61 IS 5 BP 1800 EP 1813 DI 10.1002/lno.10340 PG 14 WC Limnology; Oceanography SC Marine & Freshwater Biology; Oceanography GA DW4OD UT WOS:000383621800018 ER PT J AU Suen, AA Jefferson, WN Wood, CE Padilla-Banks, E Bae-Jump, VL Williams, CJ AF Suen, Alisa A. Jefferson, Wendy N. Wood, Charles E. Padilla-Banks, Elizabeth Bae-Jump, Victoria L. Williams, Carmen J. TI SIX1 Oncoprotein as a Biomarker in a Model of Hormonal Carcinogenesis and in Human Endometrial Cancer SO MOLECULAR CANCER RESEARCH LA English DT Article ID EPITHELIAL-MESENCHYMAL TRANSITION; BREAST-CANCER; UTERINE ADENOCARCINOMA; HOMEOPROTEIN SIX1; CERVICAL-CANCER; INFANT FORMULA; FEEDBACK LOOP; MOUSE UTERUS; EXPRESSION; EXPOSURE AB The oncofetal protein sine oculis-related homeobox 1 (SIX1) is a developmental transcription factor associated with carcinogenesis in several human cancer types but has not been investigated in human endometrial cancer. In a model of hormonal carcinogenesis, mice neonatally exposed to the soy phytoestrogen genistein (GEN) or the synthetic estrogen diethylstilbestrol (DES) develop endometrial cancer as adults. Previously, we demonstrated that SIX1 becomes aberrantly expressed in the uteri of these mice. Here, we used this mouse model to investigate the role of SIX1 expression in endometrial carcinoma development and used human tissue microarrays to explore the utility of SIX1 as a biomarker in human endometrial cancer. In mice neonatally exposed to GEN or DES, the Six1 transcript level increased dramatically over time in uteri at 6, 12, and 18 months of age and was associated with development of endometrial carcinoma. SIX1 protein localized within abnormal basal cells and all atypical hyperplastic and neoplastic lesions. These findings indicate that developmental estrogenic chemical exposure induces persistent endometrial SIX1 expression that is strongly associated with abnormal cell differentiation and cancer development. In human endometrial tissue specimens, SIX1 was not present in normal endometrium but was expressed in a subset of endometrial cancers in patients who were also more likely to have late-stage disease. These findings identify SIX1 as a disease biomarker in a model of hormonal carcinogenesis and suggest that SIX1 plays a role in endometrial cancer development in both mice and women. (C) 2016 AACR. C1 [Suen, Alisa A.; Jefferson, Wendy N.; Padilla-Banks, Elizabeth; Williams, Carmen J.] NIEHS, Reprod & Dev Biol Lab, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Suen, Alisa A.; Williams, Carmen J.] Univ N Carolina, Curriculum Toxicol, Chapel Hill, NC USA. [Wood, Charles E.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Bae-Jump, Victoria L.] Univ N Carolina, Div Gynecol Oncol, CB 7572,Phys Off Bldg,Rm B105, Chapel Hill, NC 27599 USA. [Bae-Jump, Victoria L.] Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA. RP Bae-Jump, VL (reprint author), Univ N Carolina, Div Gynecol Oncol, CB 7572,Phys Off Bldg,Rm B105, Chapel Hill, NC 27599 USA.; Williams, CJ (reprint author), NIEHS, 111 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM Victoria.Bae-Jump@unchealth.unc.edu; williamsc5@niehs.nih.gov FU Intramural Research Program of the NIH, National Institutes of Environmental Health Sciences [1ZIAES102985]; Steelman Fund; NIH/NCI K23 Mentored Patient-Oriented Research Career Development [1K23CA143154-01A1]; UNC Environmental Health Sciences Toxicology Training Grant [T32-ES007126] FX This work was supported by the Intramural Research Program of the NIH, National Institutes of Environmental Health Sciences, 1ZIAES102985 (to C.J. Williams), the Steelman Fund (to V.L. Bae-Jump), and the NIH/NCI K23 Mentored Patient-Oriented Research Career Development Grant, 1K23CA143154-01A1 (to V.L. Bae-Jump). A.A. Suen was supported in part by the UNC Environmental Health Sciences Toxicology Training Grant, T32-ES007126. NR 51 TC 1 Z9 1 U1 3 U2 3 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1541-7786 EI 1557-3125 J9 MOL CANCER RES JI Mol. Cancer Res. PD SEP PY 2016 VL 14 IS 9 BP 849 EP 858 DI 10.1158/1541-7786.MCR-16-0084 PG 10 WC Oncology; Cell Biology SC Oncology; Cell Biology GA DW0UY UT WOS:000383359700009 PM 27259717 ER PT J AU DeMayo, F AF DeMayo, Francesco TI INVESTIGATING THE MOLECULAR MECHANISMS REGULATED BY PROGESTERONE GOVERNING THE ABILITY OF THE UTERUS TO SUPPORT PREGNANCY SO PLACENTA LA English DT Meeting Abstract CT Meeting of the International-Federation-of-Placenta-Associations (IFPA) - Placenta-Back to the Basics CY SEP 13-16, 2016 CL Portland, OR SP Int Federat Placenta Assoc C1 [DeMayo, Francesco] Natl Inst Environm Hlth Sci, Reprod & Dev Biol Lab, Res Triangle Pk, NC USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU W B SAUNDERS CO LTD PI LONDON PA 32 JAMESTOWN RD, LONDON NW1 7BY, ENGLAND SN 0143-4004 EI 1532-3102 J9 PLACENTA JI Placenta PD SEP PY 2016 VL 45 MA NIH BP 72 EP 72 PG 1 WC Developmental Biology; Obstetrics & Gynecology; Reproductive Biology SC Developmental Biology; Obstetrics & Gynecology; Reproductive Biology GA DV9WX UT WOS:000383294000041 ER PT J AU Shen, HT Starr, J Han, JL Zhang, L Lu, DS Guan, RF Xu, XM Wang, XF Li, JG Li, WW Zhang, YJ Wu, YN AF Shen, Haitao Starr, James Han, Jianlong Zhang, Lei Lu, Dasheng Guan, Rongfa Xu, Xiaomin Wang, Xiaofeng Li, Jingguang Li, Weiwei Zhang, Yanjun Wu, Yongning TI The bioaccessibility of polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) in cooked plant and animal origin foods SO ENVIRONMENT INTERNATIONAL LA English DT Article DE Bioaccessibility; PCDD/Fs; PCBs; Cooked foods; In vitro assay; Dietary exposure assessment ID IN-VITRO DIGESTION; BROMINATED FLAME-RETARDANTS; EXPOSURE ASSESSMENT; DIETARY-INTAKE; SOIL; MODEL; BIOAVAILABILITY; CHINA; DUST; PESTICIDES AB In this study, we compared the effect of boiling and frying food preparation methods in determining the bioaccessibility of polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) in rice, cabbage, milk powder, eggs, beef, and fresh water fish. We then used these data to calculate a toxic equivalent (TEQ) for risk assessment and compared it to published values that did not account for bioaccessibility. When the foods were prepared by boiling, the mean bioaccessibility (%) in rice (PCBs: 16.5 +/- 1.0, PCDD/Fs: 4.9 +/- 0.3) and cabbage (PCBs: 4.2 +/- 0.9, PCDD/Fs: 1.9 +/- 0.7) were lower than in animal origin foods (beef, PCBs: 49.0 +/- 3.3, PCDD/Fs: 7.8 +/- 0.9; egg, PCBs: 29.7 +/- 3.1, PCDD/Fs: 8.6 +/- 1.3; fish, PCBs: 26.9 +/- 2.5, PCDD/Fs: 7.9 +/- 1.3; milk powder, PCBs: 72.3 +/- 1.6, PCDD/Fs: 28.4 +/- 1.2). When fried in cooking oil, the bioaccessibilities of all analytes in all foods increased, but the increase in plant based foods (rice, PCBs: 3.4x, PCDD/Fs: 3.6x; cabbage, PCBs: 10.3x, PCDD/Fs: 7.9x) was greater than that of animal origin foods (beef, PCBs: 1.6x, PCDD/Fs: 3.4x; egg, PCBs: 2.1x, PCDD/Fs: 1.8x; fish, PCBs: 2.8, PCDD/Fs: 3.2x). Comparison of PCBs/PCDD/Fs bioaccessibility in rice and cabbage showed that bioaccessibility was greater in the low fat, high carbohydrate/protein content food (rice) than in the low carbohydrate/protein, low fat content food (cabbage), regardless of the method used to prepare the food. Adjusting for bioaccessibility reduced the gross estimated daily intake (EDI) of 112pgWHO-TEQ/day, by 88% and 63% respectively for foods prepared by boiling and frying. Our results indicate that: 1) The method used for cooking is an important determinant of PCBs/PCDD/Fs bioaccessibility, especially for plant origin foods, 2) there might be a joint fat, carbohydrate and protein effect that influences the bioaccessibilities of PCBs/PCDD/Fs in foods, and 3) use of bioaccessibility estimates would reduce the uncertainty in TEQ calculations. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Shen, Haitao; Han, Jianlong; Xu, Xiaomin; Wang, Xiaofeng; Zhang, Yanjun] Zhejiang Prov Ctr Dis Control & Prevent, 3399 Binsheng Rd, Hangzhou 310051, Zhejiang, Peoples R China. [Starr, James; Li, Weiwei] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Zhang, Lei; Li, Jingguang; Wu, Yongning] China Natl Ctr Food Safety Risk Assessment, 7 Panjiayuan Nanli Rd, Beijing 100021, Peoples R China. [Lu, Dasheng] Shanghai Municipal Ctr Dis Control & Prevent, 1380 Zhongshan West Rd, Shanghai 200336, Peoples R China. [Guan, Rongfa] China Jiliang Univ, Zhejiang Prov Key Lab Biometrol & Inspect & Quara, Hangzhou 310018, Zhejiang, Peoples R China. RP Shen, HT (reprint author), Zhejiang Prov Ctr Dis Control & Prevent, 3399 Binsheng Rd, Hangzhou 310051, Zhejiang, Peoples R China.; Wu, YN (reprint author), China Natl Ctr Food Safety Risk Assessment, 7 Panjiayuan Nanli Rd, Beijing 100021, Peoples R China. EM oldfishmann@hotmail.com; wuyongning@cfsa.net.cn FU National Natural Science Foundation of China [81472986]; Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents; Zhejiang Province-National Health and Family Planning Commission of China [2015PYA003]; Shanghai Municipal Key Discipline of Public Health [15GWZK0301]; Shanghai Municipal Overseas High-End Talent Training [GWTD2015S03] FX This study was sponsored by the following foundations: 1) the National Natural Science Foundation of China (No. 81472986); 2) Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents; 3) program co-cultivated by Zhejiang Province-National Health and Family Planning Commission of China (No. 2015PYA003); 4) Shanghai Municipal Key Discipline of Public Health (No. 15GWZK0301); and 5) Shanghai Municipal Overseas High-End Talent Training (No. GWTD2015S03). The authors are very grateful to Dr. Andrew B. Lindstrom of USEPA at RTP campus for his kind revision. NR 28 TC 0 Z9 0 U1 20 U2 20 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD SEP PY 2016 VL 94 BP 33 EP 42 DI 10.1016/j.envint.2016.05.003 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DU6QF UT WOS:000382339000004 PM 27203782 ER PT J AU Brown, TN Armitage, JM Egeghy, P Kircanski, I Arnot, JA AF Brown, Trevor N. Armitage, James M. Egeghy, Peter Kircanski, Ida Arnot, Jon A. TI Dermal permeation data and models for the prioritization and screening-level exposure assessment of organic chemicals SO ENVIRONMENT INTERNATIONAL LA English DT Review DE Human exposure assessment; Dermal permeation; Quantitative structure-activity relationship; Skin permeability coefficient; Database ID PREDICTING SKIN PERMEABILITY; EXCISED HUMAN-SKIN; IN-VITRO; PERCUTANEOUS-ABSORPTION; PENETRATION ENHANCERS; CONSUMER ARTICLES; RISK-ASSESSMENT; NEAR-FIELD; VIVO; DIFFUSION AB High-throughput screening (HTS) models are being developed and applied to prioritize chemicals for more comprehensive exposure and risk assessment. Dermal pathways are possible exposure routes to humans for thousands of chemicals found in personal care products and the indoor environment. HTS exposure models rely on skin permeability coefficient (K-p; cm/h) models for exposure predictions. An initial database of approximately 1000 entries for empirically-based Kp data was compiled from the literature and a subset of 480 data points for 245 organic chemicals derived from testing with human skin only and using only water as a vehicle was selected. The selected dataset includes chemicals with log octanol-water partition coefficients (K-ow) ranging from -6.8 to 7.6 (median = 1.8; 95% of the data range from -2.5 to 4.6) and molecular weight (MW) ranging from 18 to 765 g/mol (median = 180); only 3%> 500 g/mol. Approximately 53% of the chemicals in the database have functional groups which are ionizable in the pH range of 6 to 7.4, with 31% being appreciably ionized. The compiled log Kp values ranged from -5.8 to 0.1 cm/h (median = -2.6). The selected subset of the Kp data was then used to evaluate eight representative Kp models that can be readily applied for HTS assessments, i.e., parameterized with K--ow and MW. The analysis indicates that a version of the SKINPERM model performs the best against the selected dataset. Comparisons of representative Kp models against model input parameter property ranges (sensitivity analysis) and against chemical datasets requiring human health assessment were conducted to identify regions of chemical properties that should be tested to address uncertainty in K-p models and HTS exposure assessments. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Brown, Trevor N.; Armitage, James M.; Kircanski, Ida; Arnot, Jon A.] ARC Arnot Res & Consulting Inc, 36 Sproat Ave, Toronto, ON M4M 1W4, Canada. [Armitage, James M.; Arnot, Jon A.] Univ Toronto Scarborough, Dept Phys & Environm Sci, 1265 Mil Trail, Toronto, ON M1C 1A4, Canada. [Egeghy, Peter] US EPA, Off Res & Dev, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Kircanski, Ida; Arnot, Jon A.] Univ Toronto, Dept Pharmacol & Toxicol, 1 Kings Coll Circle, Toronto, ON M5S 1A8, Canada. RP Arnot, JA (reprint author), ARC Arnot Res & Consulting Inc, 36 Sproat Ave, Toronto, ON M4M 1W4, Canada. EM jon@arnotresearch.com FU U.S. Environmental Protection Agency [EP-15-W-000068]; American Chemistry Council Long-Range Research Initiative program [6459] FX The research described in this article has been funded in part by the U.S. Environmental Protection Agency through contract EP-15-W-000068 to ARC Arnot Research and Consulting Inc. T.N.B., J.M.A., I.K. and J.A.A. also acknowledge financial support from the American Chemistry Council Long-Range Research Initiative program (Contract number 6459). This publication has not been formally reviewed by the American Chemistry Council. The views expressed in this article are those of the authors and do not necessarily reflect the views or policies of the U.S. EPA. NR 84 TC 0 Z9 0 U1 14 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD SEP PY 2016 VL 94 BP 424 EP 435 DI 10.1016/j.envint.2016.05.025 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA DU6QF UT WOS:000382339000046 PM 27282209 ER PT J AU Fantke, P Ernstoff, AS Huang, L Csiszar, SA Jolliet, O AF Fantke, Peter Ernstoff, Alexi S. Huang, Lei Csiszar, Susan A. Jolliet, Olivier TI Coupled near-field and far-field exposure assessment framework for chemicals in consumer products SO ENVIRONMENT INTERNATIONAL LA English DT Article DE Near-field consumer exposure; Multimedia multi-pathway framework; Product intake fraction; Life cycle impact assessment; Chemical alternatives assessment; High-throughput risk screening ID PERSONAL CARE PRODUCTS; IN-VITRO BIOACTIVITY; COSMETIC PRODUCTS; FRAGRANCE INGREDIENTS; DERMAL EXPOSURE; RISK-ASSESSMENT; MODEL; PRIORITIZATION; SUBSTANCES; TOXICITY AB Humans can be exposed to chemicals in consumer products through product use and environmental emissions over the product life cycle. Exposure pathways are often complex, where chemicals can transfer directly from products to humans during use or exchange between various indoor and outdoor compartments until sub-fractions reach humans. To consistently evaluate exposure pathways along product life cycles, a flexible mass balance-based assessment framework is presented structuring multimedia chemical transfers in a matrix of direct inter-compartmental transfer fractions. By matrix inversion, we quantify cumulative multimedia transfer fractions and exposure pathway-specific product intake fractions defined as chemical mass taken in by humans per unit mass of chemical in a product. Combining product intake fractions with chemical mass in the product yields intake estimates for use in life cycle impact assessment and chemical alternatives assessment, or daily intake doses for use in risk-based assessment and high-throughput screening. Two illustrative examples of chemicals used in personal care products and flooring materials demonstrate how this matrix-based framework offers a consistent and efficient way to rapidly compare exposure pathways for adult and child users and for the general population. This framework constitutes a user-friendly approach to develop, compare and interpret multiple human exposure scenarios in a coupled system of near-field ('user' environment), far-field and human intake compartments, and helps understand the contribution of individual pathways to overall human exposure in various product application contexts to inform decisions in different science-policy fields for which exposure quantification is relevant (C) 2016 The Authors. Published by Elsevier Ltd. C1 [Fantke, Peter; Ernstoff, Alexi S.] Tech Univ Denmark, Dept Engn Management, Quantitat Sustainabil Assessment Div, Prod Storvet 424, DK-2800 Lyngby, Denmark. [Huang, Lei; Jolliet, Olivier] Univ Michigan, Environm Hlth Sci, 1415 Washington Hts, Ann Arbor, MI 48109 USA. [Csiszar, Susan A.] US EPA, Oak Ridge Inst Sci & Educ, Ncit Risk Management Res Lab, Cincinnati, OH 45268 USA. RP Fantke, P (reprint author), Tech Univ Denmark, Dept Engn Management, Quantitat Sustainabil Assessment Div, Prod Storvet 424, DK-2800 Lyngby, Denmark. EM pefan@dtu.dk RI Ernstoff, Alexi/P-4728-2016; OI Ernstoff, Alexi/0000-0002-1114-6596; Fantke, Peter/0000-0001-7148-6982 FU European Commission under the Seventh Framework Programme [631910, 285286]; U.S. EPA on Development of Modular Risk Pathway Descriptions for Life Cycle Assessment [EP-14-C-000115]; Long Range Research Initiative of the American Chemistry Council [MTH1001-01]; U.S. Department of Energy [DW-89-92298301]; U.S. EPA [DW-89-92298301] FX We thank Peter Egeghy, Debbie Bennett, Jane Bare, and Jon Arnot for initial comments. This work was supported by the Marie Curie projects Quan-Tox (GA No. 631910) and Tox-Train (GA No. 285286) funded by the European Commission under the Seventh Framework Programme, by the U.S. EPA contract EP-14-C-000115 on Development of Modular Risk Pathway Descriptions for Life Cycle Assessment and the Long Range Research Initiative of the American Chemistry Council (MTH1001-01), and partly by an appointment to the Postdoctoral Research Program at the National Risk Management Research Laboratory, U.S. EPA administered by the Oak Ridge Institute for Science and Education (IAG No. DW-89-92298301 between the U.S. Department of Energy and the U.S. EPA). The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. EPA. NR 49 TC 1 Z9 1 U1 10 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD SEP PY 2016 VL 94 BP 508 EP 518 DI 10.1016/j.envint.2016.06.010 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DU6QF UT WOS:000382339000056 PM 27318619 ER PT J AU Ferguson, KK Meeker, JD Cantonwine, DE Chen, YH Mukherjee, B McElrath, TF AF Ferguson, Kelly K. Meeker, John D. Cantonwine, David E. Chen, Yin-Hsiu Mukherjee, Bhramar McElrath, Thomas F. TI Urinary phthalate metabolite and bisphenol A associations with ultrasound and delivery indices of fetal growth SO ENVIRONMENT INTERNATIONAL LA English DT Article DE Endocrine disruptors; Fetal growth; Birthweight; Growth restriction; Plasticizers; Plastics ID LOW-BIRTH-WEIGHT; PRENATAL EXPOSURE; PRETERM BIRTH; GENERATION R; OXIDATIVE STRESS; PREGNANT-WOMEN; IN-VITRO; OUTCOMES; HEALTH; PHENOLS AB Growth of the fetus is highly sensitive to environmental perturbations, and disruption can lead to problems in pregnancy as well as later in life. This study investigates the relationship between maternal exposure to common plasticizers in pregnancy and fetal growth. Participants from a longitudinal birth cohort in Boston were recruited early in gestation and followed until delivery. Urine samples were collected at up to four time points and analyzed for concentrations of phthalate metabolites and bisphenol A (BPA). Ultrasound scans were performed at four time points during pregnancy for estimation of growth parameters, and birthweight was recorded at delivery. Growth measures were standardized to a larger population. For the present analysis we examined cross-sectional and repeated measures associations between exposure biomarkers and growth estimates in 482 non anomalous singleton pregnancies. Cross-sectional associations between urinary phthalate metabolites or BPA and growth indices were imprecise. However, in repeated measures models, we observed significant inverse associations between di-2-ethylhexyl phthalate (DEHP) metabolites and estimated or actual fetal weight. An inter quartile range increase in summed DEHP metabolites was associated with a 0.13 standard deviation decrease in estimated or actual fetal weight (95% confidence interval = -0.23, -0.03). Associations were consistent across different growth parameters (e.g., head circumference, femur length), and by fetal sex. No consistent associations were observed for other phthalate metabolites or BPA. Maternal exposure to DEHP during pregnancy was associated with decreased fetal growth, which could have repercussive effects. Published by Elsevier Ltd. C1 [Ferguson, Kelly K.] Natl Inst Environm Hlth Sci, Epidemiol Branch, 111 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Ferguson, Kelly K.; Meeker, John D.] Univ Michigan, Sch Publ Hlth, Dept Environm Hlth Sci, Ann Arbor, MI 48109 USA. [Cantonwine, David E.; McElrath, Thomas F.] Harvard Med Sch, Brigham & Womens Hosp, Div Maternal Fetal Med, Boston, MA USA. [Chen, Yin-Hsiu; Mukherjee, Bhramar] Univ Michigan, Sch Publ Hlth, Dept Biostat, Ann Arbor, MI 48109 USA. RP Ferguson, KK (reprint author), Natl Inst Environm Hlth Sci, Epidemiol Branch, 111 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM kelly.ferguson2@nih.gov OI Ferguson, Kelly/0000-0001-8467-3250 FU Abbott Diagnostics [9MZ-04-06N03]; National Institute of Environmental Health Sciences, National Institutes of Health [R01ES018872, P42ES017198, P01ES022844, P50ES026049, P30ES017885]; Intramural Research Program of the NIH, National Institute of Environmental Health Sciences FX We thank Gerry Pace and colleagues at NSF International (Ann Arbor, MI) for their analysis of urinary phthalate metabolites. Initial funding for the recruitment of the birth cohort was provided by Abbott Diagnostics (9MZ-04-06N03). All other funding was provided by the National Institute of Environmental Health Sciences, National Institutes of Health (R01ES018872, P42ES017198, P01ES022844, P50ES026049, and P30ES017885). Support for Dr. Ferguson was provided in part by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences. NR 49 TC 0 Z9 0 U1 13 U2 13 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD SEP PY 2016 VL 94 BP 531 EP 537 DI 10.1016/j.envint.2016.06.013 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA DU6QF UT WOS:000382339000059 PM 27320326 ER PT J AU Greaver, TL Clark, CM Compton, JE Vallano, D Talhelm, AF Weaver, CP Band, LE Baron, JS Davidson, EA Tague, CL Felker-Quinn, E Lynch, JA Herrick, JD Liu, L Goodale, CL Novak, KJ Haeuber, RA AF Greaver, T. L. Clark, C. M. Compton, J. E. Vallano, D. Talhelm, A. F. Weaver, C. P. Band, L. E. Baron, J. S. Davidson, E. A. Tague, C. L. Felker-Quinn, E. Lynch, J. A. Herrick, J. D. Liu, L. Goodale, C. L. Novak, K. J. Haeuber, R. A. TI Key ecological responses to nitrogen are altered by climate change SO NATURE CLIMATE CHANGE LA English DT Review ID SHALLOW LAKES; UNITED-STATES; TERRESTRIAL ECOSYSTEMS; SOIL RESPIRATION; ELEVATED CO2; FRESH-WATER; AQUATIC ECOSYSTEMS; FOREST ECOSYSTEMS; ACIDIC DEPOSITION; REACTIVE NITROGEN AB Climate change and anthropogenic nitrogen deposition are both important ecological threats. Evaluating their cumulative effects provides a more holistic view of ecosystem vulnerability to human activities, which would better inform policy decisions aimed to protect the sustainability of ecosystems. Our knowledge of the cumulative effects of these stressors is growing, but we lack an integrated understanding. In this Review, we describe how climate change alters key processes in terrestrial and freshwater ecosystems related to nitrogen cycling and availability, and the response of ecosystems to nitrogen addition in terms of carbon cycling, acidification and biodiversity. C1 [Greaver, T. L.; Talhelm, A. F.; Weaver, C. P.; Felker-Quinn, E.; Herrick, J. D.; Novak, K. J.] US EPA, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. [Clark, C. M.] US EPA, Natl Ctr Environm Assessment, Arlington, VA 22202 USA. [Compton, J. E.] US EPA, Western Ecol Div, Corvallis, OR 97333 USA. [Vallano, D.] US EPA, AQAO, Reg 9,75 Hawthorne St, San Francisco, CA 94105 USA. [Talhelm, A. F.] Univ Idaho, Coll Nat Resources, Moscow, ID 83844 USA. [Band, L. E.] Univ North Carolina Chapel Hill, Inst Environm, Dept Geog, Chapel Hill, NC 27514 USA. [Baron, J. S.] Colorado State Univ, US Geol Survey, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Davidson, E. A.] Univ Maryland, Appalachian Lab, Ctr Environm Sci, 301 Braddock Rd, Frostburg, MD 21532 USA. [Tague, C. L.] Univ Calif, Bren Sch Environm Sci & Management, Bren Hall, Isla Vista, CA 93271 USA. [Lynch, J. A.; Haeuber, R. A.] US EPA, Clean Air Markets Div, Washington, DC 20460 USA. [Liu, L.] Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China. [Goodale, C. L.] Cornell Univ, Ecol & Evolutionary Biol, Corson Hall Ithaca, Ithaca, NY 14853 USA. RP Greaver, TL (reprint author), US EPA, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. EM greaver.tara@epa.gov RI Weaver, Christopher/G-3714-2010; Davidson, Eric/K-4984-2013 OI Weaver, Christopher/0000-0003-4016-5451; Davidson, Eric/0000-0002-8525-8697 FU Environmental Protection Agency FX The authors thank the participants of the Environmental Protection Agency sponsored workshop: 'Interacting Effects of Climate and Nitrogen on Ecosystems and Their Services: Workshop to Review Current Science and Inform Policy-Driven Scientific Needs' for their contributions. We also thank Meredith Lassiter and Ellen Cooter for technical comments to improve the manuscript. The views expressed in this abstract are those of the authors and do not necessarily represent the views or policies of the US EPA. NR 100 TC 2 Z9 2 U1 110 U2 110 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD SEP PY 2016 VL 6 IS 9 BP 836 EP 843 DI 10.1038/NCLIMATE3088 PG 8 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DU3UB UT WOS:000382136600012 ER PT J AU Harding, LB Schultz, IR da Silva, DAM Ylitalo, GM Ragsdale, D Harris, SI Bailey, S Pepich, BV Swanson, P AF Harding, Louisa B. Schultz, Irvin R. da Silva, Denis A. M. Ylitalo, Gina M. Ragsdale, Dave Harris, Stephanie I. Bailey, Stephanie Pepich, Barry V. Swanson, Penny TI Wastewater treatment plant effluent alters pituitary gland gonadotropin mRNA levels in juvenile coho salmon (Oncorhynchus kisutch) SO AQUATIC TOXICOLOGY LA English DT Article DE Endocrine disrupting compound; Pituitary; Gonadotropin; Luteinizing hormone; Follicle-stimulating hormone; Vitellogenin; Wastewater effluent ID GOLDFISH CARASSIUS-AURATUS; FEMALE RAINBOW-TROUT; BETA SUBUNIT GENE; FOLLICLE-STIMULATING-HORMONE; CATFISH CLARIAS-GARIEPINUS; PERSONAL CARE PRODUCTS; SEWAGE-TREATMENT PLANT; GTH-II SECRETION; MASS-SPECTROMETRY; ATLANTIC SALMON AB It is well known that endocrine disrupting compounds (EDCs) present in wastewater treatment plant (WWTP) effluents interfere with reproduction in fish, including altered gonad development and induction of vitellogenin (Vtg), a female-specific egg yolk protein precursor produced in the liver. As a result, studies have focused on the effects of EDC exposure on the gonad and liver. However, impacts of environmental EDC exposure at higher levels of the hypothalamic-pituitary-gonad axis are less well understood. The pituitary gonadotropins, follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) are involved in all aspects of gonad development and are subject to feedback from gonadal steroids making them a likely target of endocrine disruption. In this study, the effects of WWTP effluent exposure on pituitary gonadotropin mRNA expression were investigated to assess the utility of Lh beta-subunit (lhb) as a biomarker of estrogen exposure in juvenile coho salmon (Oncorhynchus kisutch). First, a controlled 72-h exposure to 17 alpha-ethynylestradiol (EE2) and 17 beta-trenbolone (TREN) was performed to evaluate the response of juvenile coho salmon to EDC exposure. Second, juvenile coho salmon were exposed to 0, 20 or 100% effluent from eight WWTPs from the Puget Sound, WA region for 72 h. Juvenile coho salmon exposed to 2 and 10 ng EE2 L-1 had 17-fold and 215-fold higher lhb mRNA levels relative to control fish. Hepatic vtg mRNA levels were dramatically increased 6670-fold, but only in response to 10 ng EE2 L-1 and Fsh beta-subunit (fshb) mRNA levels were not altered by any of the treatments. In the WWTP effluent exposures, lhb mRNA levels were significantly elevated in fish exposed to five of the WWTP effluents. In contrast, transcript levels of vtg were not affected by any of the WWTP effluent exposures. Mean levels of natural and synthetic estrogens in fish bile were consistent with pituitary lhb expression, suggesting that the observed lhb induction may be due to estrogenic activity of the WWTP effluents. These results suggest that lhb gene expression may be a sensitive index of acute exposure to estrogenic chemicals in juvenile coho salmon. Further work is needed to determine the kinetics and specificity of lhb induction to evaluate its utility as a potential indicator of estrogen exposure in immature fish. Published by Elsevier B.V. C1 [Harding, Louisa B.] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. [Schultz, Irvin R.] Pacific Northwest Natl Lab, Marine Sci Lab, 1529 West Sequim Bay Rd, Sequim, WA 98382 USA. [da Silva, Denis A. M.; Ylitalo, Gina M.; Swanson, Penny] Natl Ocean & Atmospher Adm, Northwest Fisheries Sci Ctr, Natl Marine Fisheries Serv, 2725 Montlake Blvd E, Seattle, WA 98112 USA. [Ragsdale, Dave; Harris, Stephanie I.; Bailey, Stephanie; Pepich, Barry V.] US EPA, Manchester Environm Lab, Reg 10,7411 Beach Dr E, Port Orchard, WA 98366 USA. [Swanson, Penny] Washington State Univ, Ctr Reprod Biol, Pullman, WA 98164 USA. [Ragsdale, Dave] POB 88, Ophir, OR 97464 USA. RP Swanson, P (reprint author), Natl Ocean & Atmospher Adm, Northwest Fisheries Sci Ctr, Natl Marine Fisheries Serv, 2725 Montlake Blvd E, Seattle, WA 98112 USA. EM penny.swanson@noaa.gov FU National Oceanic and Atmospheric Administration; US Environmental Protection Agency, Region 10, Puget Sound Science and Technical Studies Assistance Program [EPA R10-PS-1004, 13-923270-01]; Richard T. Whiteleather scholarship; Melvin Anderson Endowed Scholarship in Fisheries; Roy Jensen Research Fellowship; Lauren R. Donaldson Scholarship FX Funds for this work were provided by National Oceanic and Atmospheric Administration and the US Environmental Protection Agency, Region 10, Puget Sound Science and Technical Studies Assistance Program (EPA R10-PS-1004, federal grant no. 13-923270-01) and by scholarships to Louisa Harding from the Richard T. Whiteleather scholarship, the Melvin Anderson Endowed Scholarship in Fisheries, the Roy Jensen Research Fellowship, and the Lauren R. Donaldson Scholarship. The authors wish to acknowledge Abby Furhman, Chris Monson, Elizabeth Smith and Richard Edmunds for technical assistance with fish care, sampling, and statistical analyses. The authors also wish to thank Dan Villeneuve, David Bencic, and James Lazorchak for providing helpful feedback on earlier versions of this manuscript. NR 98 TC 0 Z9 0 U1 23 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-445X EI 1879-1514 J9 AQUAT TOXICOL JI Aquat. Toxicol. PD SEP PY 2016 VL 178 BP 118 EP 131 DI 10.1016/j.aquatox.2016.07.013 PG 14 WC Marine & Freshwater Biology; Toxicology SC Marine & Freshwater Biology; Toxicology GA DV3DV UT WOS:000382802100013 PM 27475653 ER PT J AU Sarwar, G Kang, DW Foley, K Schwede, D Gantt, B Mathur, R AF Sarwar, Golam Kang, Daiwen Foley, Kristen Schwede, Donna Gantt, Brett Mathur, Rohit TI Technical note: Examining ozone deposition over seawater SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Iodide; Dimethylsulfide; Dissolved organic carbon; Ozone; Deposition ID MARINE BOUNDARY-LAYER; DISSOLVED ORGANIC-CARBON; HENRYS LAW CONSTANT; SEA-SURFACE; DIMETHYLSULFIDE DMS; NORTHERN-HEMISPHERE; HALOGEN CHEMISTRY; INORGANIC IODINE; FREE TROPOSPHERE; WATER AB Surface layer resistance plays an important role in determining ozone deposition velocity over sea-water and can be influenced by chemical interactions at the air-water interface. Here, we examine the effect of chemical interactions of iodide, dimethylsulfide, dissolved organic carbon, and bromide in seawater on ozone deposition. We perform a series of simulations using the hemispheric Community Multiscale Air Quality model for summer months in the Northern Hemisphere. Our results suggest that each chemical interaction enhances the ozone deposition velocity and decreases the atmospheric ozone mixing ratio over seawater. Iodide enhances the median deposition velocity over seawater by 0.023 cm s(-1), dissolved organic carbon by 0.021 cm s(-1), dimethylsulfide by 0.002 cm s(-1), and bromide by similar to 0.0006 cm s(-1). Consequently, iodide decreases the median atmospheric ozone mixing ratio over seawater by 0.7 ppb, dissolved organic carbon by 0.8 ppb, dimethylsulfide by 0.1 ppb, and bromide by 0.02 ppb. In a separate model simulation, we account for the effect of dissolved salts in seawater on the Henry's law constant for ozone and find that it reduces the median deposition velocity by 0.007 cm s(-1) and increases surface ozone mixing ratio by 0.2 ppb. The combined effect of these processes increases the median ozone deposition velocity over seawater by 0.040 cm s(-1), lowers the atmospheric ozone mixing ratio by 5%, and slightly improves model performance relative to observations. Published by Elsevier Ltd. C1 [Sarwar, Golam; Kang, Daiwen; Foley, Kristen; Schwede, Donna; Mathur, Rohit] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Gantt, Brett] US EPA, Off Air Qual Planning & Stand, Res Triangle Pk, NC USA. RP Sarwar, G (reprint author), US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. EM sarwar.golam@epa.gov NR 51 TC 1 Z9 1 U1 7 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD SEP PY 2016 VL 141 BP 255 EP 262 DI 10.1016/j.atmosenv.2016.06.072 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DU1EL UT WOS:000381950900023 ER PT J AU Zheng, X Wu, Y Zhang, SJ Baldauf, RW Zhang, KM Hu, JN Li, ZH Fu, LX Hao, JM AF Zheng, Xuan Wu, Ye Zhang, Shaojun Baldauf, Richard W. Zhang, K. Max Hu, Jingnan Li, Zhenhua Fu, Lixin Hao, Jiming TI Joint measurements of black carbon and particle mass for heavy-duty diesel vehicles using a portable emission measurement system SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Portable emission measurement system; Real-world emission; Heavy-duty diesel vehicle; Black carbon; Particle mass ID WORLD FUEL CONSUMPTION; LOW-PRESSURE IMPACTOR; SIZE DISTRIBUTIONS; CHINESE CITIES; IN-USE; EXHAUST; AEROSOLS; ENGINE; BUSES; NOX AB The black carbon (BC) emitted from heavy-duty diesel vehicles (HDDVs) is an important source of urban atmospheric pollution and creates strong climate-forcing impacts. The emission ratio of BC to total particle mass (PM) (i.e., BC/PM ratio) is an essential variable used to estimate total BC emissions from historical PM data; however, these ratios have not been measured using portable emission measurement systems (PEMS) in order to obtain real-world measurements over a wide range of driving conditions. In this study, we developed a PEMS platform by integrating two Aethalometers and an electric low pressure impactor to realize the joint measurement of real-world BC and PM emissions for ten HDDVs in China. Test results showed that the average BC/PM ratio for five HDDVs equipped with mechanical fuel injection (MI) engines was 0.43 +/- 0.06, significantly lower (P < 0.05) than another five HDDVs equipped with electronically-controlled fuel injection (EI) engines (0.56 +/- 0.12). Traffic conditions also affected the BC/PM ratios with higher ratios on freeway routes than on local roads. Furthermore, higher ratios were observed for HDDVs equipped with EI engines than for the MI engines for the highway and local road routes. With an operating mode binning approach, we observed that the instantaneous BC/PM ratios of El engine vehicles were above those of the MI engine vehicles in all operating modes except for the braking mode (i.e., Bin 0). Therefore, the complex impacts from engine technology and traffic conditions on BC/PM ratios should be carefully considered when estimating real-world BC emissions from HDDVs based on overall PM emissions data. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Zheng, Xuan; Wu, Ye; Li, Zhenhua; Fu, Lixin; Hao, Jiming] Tsinghua Univ, State Key Joint Lab Environm Simulat & Pollut Con, Sch Environm, Beijing 100084, Peoples R China. [Zheng, Xuan; Wu, Ye; Fu, Lixin; Hao, Jiming] State Environm Protect Key Lab Sources & Control, Beijing 100084, Peoples R China. [Zhang, Shaojun] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA. [Baldauf, Richard W.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Baldauf, Richard W.] US EPA, Off Transportat & Air Qual, Natl Vehicle & Fuels Emiss Lab, Ann Arbor, MI 48105 USA. [Zhang, K. Max] Cornell Univ, Sibley Sch Mech & Aerosp Engn, Ithaca, NY 14853 USA. [Hu, Jingnan] Chinese Res Inst Environm Sci, State Environm Protect Key Lab Vehicle Pollut Con, Beijing 100012, Peoples R China. RP Wu, Y (reprint author), Tsinghua Univ, State Key Joint Lab Environm Simulat & Pollut Con, Sch Environm, Beijing 100084, Peoples R China. EM ywu@tsinghua.edu.cn RI Zhang, Shaojun/E-7745-2016; Wu, Ye/O-9779-2015 OI Zhang, Shaojun/0000-0002-2176-6174; FU National Science & Technology Pillar Program of China [2013BAC13B03]; Environmental Public Welfare Project [201409013]; National Natural Science Foundation of China [51322804, 91544222] FX This work was supported by the National Science & Technology Pillar Program of China (2013BAC13B03), the Environmental Public Welfare Project (201409013), and the National Natural Science Foundation of China (51322804 and 91544222). The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of the sponsors. NR 54 TC 2 Z9 2 U1 20 U2 22 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD SEP PY 2016 VL 141 BP 435 EP 442 DI 10.1016/j.atmosenv.2016.07.013 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DU1EL UT WOS:000381950900040 ER PT J AU Venkatram, A Isakov, V Des'hmukh, P Baldauf, R AF Venkatram, Akula Isakov, Vlad Des'hmukh, Parikshit Baldauf, Richard TI Modeling the impact of solid noise barriers on near road air quality SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Air quality; Dispersion model; Noise barrier; Field data; Emission factors ID NEAR-ROAD; DISPERSION; EMISSIONS AB Studies based on field measurements, wind tunnel experiments, and controlled tracer gas releases indicate that solid, roadside noise barriers can lead to reductions in downwind near-road air pollutant concentrations. A tracer gas study showed that a solid barrier reduced pollutant concentrations as much as 80% next to the barrier relative to an open area under unstable meteorological conditions, which corresponds to typical daytime conditions when residents living or children going to school near roadways are most likely to be exposed to traffic emissions. The data from this tracer gas study and a wind tunnel simulation were used to develop a model to describe dispersion of traffic emissions near a highway in the presence of a solid noise barrier. The model is used to interpret real-world data collected during a field study conducted in a complex urban environment next to a large highway in Phoenix, Arizona, USA. We show that the analysis of the data with the model yields useful information on the emission factors and the mitigation impact of the barrier on near-road air quality. The estimated emission factors for the four species, ultrafine particles, CO, NO2, and black carbon, are consistent with data cited in the literature. The results suggest that the model accounted for reductions in pollutant concentrations from a 4.5 m high noise barrier, ranging from 40% next to the barrier to 10% at 300 m from the barrier. Published by Elsevier Ltd. C1 [Venkatram, Akula] Univ Calif Riverside, Riverside, CA USA. [Isakov, Vlad] US EPA, Atmospher Modeling & Anal Div, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC USA. [Des'hmukh, Parikshit] Jacobs Technol, Durham, NC USA. [Baldauf, Richard] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC USA. [Baldauf, Richard] US EPA, Off Transportat & Air Qual, Natl Vehicle & Fuels Emiss Lab, Ann Arbor, MI USA. RP Baldauf, R (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC USA. EM Baldauf.Richard@epa.gov FU U.S. Environmental Protection Agency FX This study was fully funded by the U.S. Environmental Protection Agency. We thank Ben Davis and the Maricopa County Air Quality Department for graciously providing storage and maintenance facilities for the air quality monitoring equipment. We also thank Wang Zhang and Vladimir Livshits of the Maricopa Association of Governments for traffic data on I-17 during the sampling period, and historical data on fleet mix characteristics for these sections of I-17 in Phoenix. In addition, we thank Darcy Anderson and Beverly Chenausky of the Arizona Department of Transportation for providing insights on sampling locations and assistance with permitting for access to the sampling locations. We finally want to thank Halley Brantley and Gayle Hagler for their assistance in data processing and quality assurance analyses. NR 14 TC 1 Z9 1 U1 13 U2 13 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD SEP PY 2016 VL 141 BP 462 EP 469 DI 10.1016/j.atmosenv.2016.07.005 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DU1EL UT WOS:000381950900043 ER PT J AU Simon, H Wells, B Baker, KR Hubbell, B AF Simon, Heather Wells, Benjamin Baker, Kirk R. Hubbell, Bryan TI Assessing Temporal and Spatial Patterns of Observed and Predicted Ozone in Multiple Urban Areas SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID NORTHEASTERN UNITED-STATES; SENSITIVITY-ANALYSIS; AIR-POLLUTION; MORTALITY; HYDROCARBONS; VARIABILITY; EXPOSURE; TRENDS; ORDER; NOX AB BACKGROUND: Ambient monitoring data show spatial gradients in ozone (O-3) across urban areas. Nitrogen oxide (NOx) emissions reductions will likely alter these gradients. Epidemiological studies often use exposure surrogates that may not fully account for the impacts of spatially and temporally changing concentrations on population exposure. OBJECTIVES: We examined the impact of large NOx decreases on spatial and temporal O-3 patterns and the implications on exposure. METHODS: We used a photochemical model to estimate O-3 response to large NOx reductions. We derived time series of 2006-2008 O-3 concentrations consistent with 50% and 75% NOx emissions reduction scenarios in three urban areas (Atlanta, Philadelphia, and Chicago) at each monitor location and spatially interpolated O-3 to census-tract centroids. RESULTS: We predicted that low O-3 concentrations would increase and high O-3 concentrations would decrease in response to NOx reductions within an urban area. O-3 increases occurred across larger areas for the seasonal mean metric than for the regulatory metric (annual 4th highest daily 8-hr maximum) and were located only in urban core areas. O-3 always decreased outside the urban core (e.g., at locations of maximum local ozone concentration) for both metrics and decreased within the urban core in some instances. NOx reductions led to more uniform spatial gradients and diurnal and seasonal patterns and caused seasonal peaks in midrange O-3 concentrations to shift from midsummer to earlier in the year. CONCLUSIONS: These changes have implications for how O-3 exposure may change in response to NOx reductions and are informative for the design of future epidemiology studies and risk assessments. C1 [Simon, Heather; Wells, Benjamin; Baker, Kirk R.; Hubbell, Bryan] US EPA, Off Air Qual Planning & Stand, Res Triangle Pk, NC 27711 USA. RP Simon, H (reprint author), 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM simon.heather@epa.gov RI simon, heather/E-4392-2011; OI simon, heather/0000-0001-7254-3360; Hubbell, Bryan/0000-0002-7963-3438 NR 31 TC 1 Z9 1 U1 7 U2 9 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD SEP PY 2016 VL 124 IS 9 BP 1443 EP 1452 DI 10.1289/EHP190 PG 10 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DU9IM UT WOS:000382530200025 PM 27153213 ER PT J AU Bhhatarai, B Wilson, DM Price, PS Marty, S Parks, AK Carney, E AF Bhhatarai, Barun Wilson, Daniel M. Price, Paul S. Marty, Sue Parks, Amanda K. Carney, Edward TI Evaluation of OASIS QSAR Models Using ToxCast (TM) in Vitro Estrogen and Androgen Receptor Binding Data and Application in an Integrated Endocrine Screening Approach SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID IDENTIFICATION ALGORITHM; ENVIRONMENTAL CHEMICALS; DISRUPTING CHEMICALS; LIGANDS; ALPHA; PREDICTION; TOXICITY; AFFINITY; PROGRAM; ASSAYS AB BACKGROUND: Integrative testing strategies (ITSs) for potential endocrine activity can use tiered in silico and in vitro models. Each component of an ITS should be thoroughly assessed. OBJECTIVES: We used the data from three in vitro ToxCast (TM) binding assays to assess OASIS, a quantitative structure-activity relationship (QSAR) platform covering both estrogen receptor (ER) and androgen receptor (AR) binding. For stronger binders (described here as AC(50) < 1 mu M), we also examined the relationship of QSAR predictions of ER or AR binding to the results from 18 ER and 10 AR transactivation assays, 72 ER-binding reference compounds, and the in vivo uterotrophic assay. METHODS: NovaScreen binding assay data for ER (human, bovine, and mouse) and AR (human, chimpanzee, and rat) were used to assess the sensitivity, specificity, concordance, and applicability domain of two OASIS QSAR models. The binding strength relative to the QSAR-predicted binding strength was examined for the ER data. The relationship of QSAR predictions of binding to transactivation- and pathway-based assays, as well as to in vivo uterotrophic responses, was examined. RESULTS: The QSAR models had both high sensitivity (> 75%) and specificity (> 86%) for ER as well as both high sensitivity (92-100%) and specificity (70-81%) for AR. For compounds within the domains of the ER and AR QSAR models that bound with AC(50) < 1 mu M, the QSAR models accurately predicted the binding for the parent compounds. The parent compounds were active in all transactivation assays where metabolism was incorporated and, except for those compounds known to require metabolism to manifest activity, all assay platforms where metabolism was not incorporated. Compounds in-domain and predicted to bind by the ER QSAR model that were positive in ToxCast (TM) ER binding at AC(50) < 1 mu M were active in the uterotrophic assay. CONCLUSIONS: We used the extensive ToxCast (TM) HTS binding data set to show that OASIS ER and AR QSAR models had high sensitivity and specificity when compounds were in-domain of the models. Based on this research, we recommend a tiered screening approach wherein a) QSAR is used to identify compounds in-domain of the ER or AR binding models and predicted to bind; b) those compounds are screened in vitro to assess binding potency; and c) the stronger binders (AC(50) < 1 mu M) are screened in vivo. This scheme prioritizes compounds for integrative testing and risk assessment. Importantly, compounds that are not in-domain, that are predicted either not to bind or to bind weakly, that are not active in in vitro, that require metabolism to manifest activity, or for which in vivo AR testing is in order, need to be assessed differently. C1 [Bhhatarai, Barun; Wilson, Daniel M.; Price, Paul S.; Marty, Sue; Parks, Amanda K.; Carney, Edward] Dow Chem Co USA, Toxicol Environm Res & Consulting, Midland, MI 48674 USA. [Marty, Sue] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. RP Bhhatarai, B (reprint author), Dow Chem Co USA, Midland, MI 48674 USA. EM bbhhatarai@dow.com NR 24 TC 0 Z9 0 U1 13 U2 13 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD SEP PY 2016 VL 124 IS 9 BP 1453 EP 1461 DI 10.1289/EHP184 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DU9IM UT WOS:000382530200026 PM 27152837 ER PT J AU von Lindern, I Spalinger, S Stifelman, ML Stanek, LW Bartrem, C AF von Lindern, Ian Spalinger, Susan Stifelman, Marc L. Stanek, Lindsay Wichers Bartrem, Casey TI Estimating Children's Soil/Dust Ingestion Rates through Retrospective Analyses of Blood Lead Biomonitoring from the Bunker Hill Superfund Site in Idaho SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID SOIL INGESTION; DUST INGESTION; SILVER VALLEY; MASS-BALANCE; HOUSE-DUST; EXPOSURE; SMELTER; ADULTS; RISK AB BACKGROUND: Soil/dust ingestion rates are important variables in assessing children's health risks in contaminated environments. Current estimates are based largely on soil tracer methodology, which is limited by analytical uncertainty, small sample size, and short study duration. OBJECTIVES: The objective was to estimate site-specific soil/dust ingestion rates through reevaluation of the lead absorption dose-response relationship using new bioavailability data from the Bunker Hill Mining and Metallurgical Complex Superfund Site (BHSS) in Idaho, USA. METHODS: The U.S. Environmental Protection Agency (EPA) in vitro bioavailability methodology was applied to archived BHSS soil and dust samples. Using age-specific biokinetic slope factors, we related bioavailable lead from these sources to children's blood lead levels (BLLs) monitored during cleanup from 1988 through 2002. Quantitative regression analyses and exposure assessment guidance were used to develop candidate soil/dust source partition scenarios estimating lead intake, allowing estimation of age-specific soil/dust ingestion rates. These ingestion rate and bioavailability estimates were simultaneously applied to the U.S. EPA Integrated Exposure Uptake Biokinetic Model for Lead in Children to determine those combinations best approximating observed BLLs. RESULTS: Absolute soil and house dust bioavailability averaged 33% (SD +/- 4%) and 28% (SD +/- 6%), respectively. Estimated BHSS age-specific soil/dust ingestion rates are 86-94 mg/day for 6-month- to 2-year-old children and 51-67 mg/day for 2- to 9-year-old children. CONCLUSIONS: Soil/dust ingestion rate estimates for 1- to 9-year-old children at the BHSS are lower than those commonly used in human health risk assessment. A substantial component of children's exposure comes from sources beyond the immediate home environment. C1 [von Lindern, Ian; Spalinger, Susan; Bartrem, Casey] TerraGraph Environm Engn Inc, 121 S Jackson St, Moscow, ID 83843 USA. [Stifelman, Marc L.] US EPA, Reg 10, Seattle, WA USA. [Stanek, Lindsay Wichers] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Spalinger, S (reprint author), TerraGraph Environm Engn Inc, 121 S Jackson St, Moscow, ID 83843 USA. EM susan.spalinger@terragraphics.com FU U.S. EPA; Idaho Department of Environmental Quality (IDEQ); TerraGraphics FX Funding for this work was provided by the U.S. EPA, by the Idaho Department of Environmental Quality (IDEQ), and by TerraGraphics. NR 51 TC 2 Z9 2 U1 13 U2 13 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD SEP PY 2016 VL 124 IS 9 BP 1462 EP 1470 DI 10.1289/ehp.1510144 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DU9IM UT WOS:000382530200027 PM 26745545 ER PT J AU Mehan, GT Carpenter, AT Via, S AF Mehan, G. Tracy, III Carpenter, Adam T. Via, Steve TI The Expanding Role of the Utility SO JOURNAL AMERICAN WATER WORKS ASSOCIATION LA English DT Article C1 [Mehan, G. Tracy, III] AWWA Govt Affairs Off, Govt Affairs, Washington, DC 20005 USA. [Mehan, G. Tracy, III] US EPA, Water, Washington, DC 20460 USA. [Mehan, G. Tracy, III] George Mason Univ, Sch Law, Arlington, VA USA. [Mehan, G. Tracy, III] Carnegie Mellon Heinz Coll, Pittsburgh, PA USA. [Carpenter, Adam T.] AWWA Govt Affairs Off, Washington, DC USA. [Via, Steve] AWWA Govt Affairs Off, Fed Regulat, Washington, DC USA. RP Mehan, GT (reprint author), AWWA Govt Affairs Off, Govt Affairs, Washington, DC 20005 USA. EM tmehan@awwa.org NR 7 TC 0 Z9 0 U1 0 U2 0 PU AMER WATER WORKS ASSOC PI DENVER PA 6666 W QUINCY AVE, DENVER, CO 80235 USA SN 2164-4535 J9 J AM WATER WORKS ASS JI J. Am. Water Work Assoc. PD SEP PY 2016 VL 108 IS 9 BP 12 EP + DI 10.5942/jawwa.2016.108.0179 PG 4 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA DU7YM UT WOS:000382430500003 ER PT J AU Fewell, B AF Fewell, Brent TI Encouraging Greater Compliance Requires a Change in the Status Quo SO JOURNAL AMERICAN WATER WORKS ASSOCIATION LA English DT Article C1 [Fewell, Brent] Earth & Water Grp, 1455 Penn Ave,Ste 400, Washington, DC 20004 USA. [Fewell, Brent] US EPA, Off Water, Washington, DC USA. RP Fewell, B (reprint author), Earth & Water Grp, 1455 Penn Ave,Ste 400, Washington, DC 20004 USA. EM brent.fewell@earthandwatergroup.com NR 12 TC 0 Z9 0 U1 1 U2 1 PU AMER WATER WORKS ASSOC PI DENVER PA 6666 W QUINCY AVE, DENVER, CO 80235 USA SN 2164-4535 J9 J AM WATER WORKS ASS JI J. Am. Water Work Assoc. PD SEP PY 2016 VL 108 IS 9 BP 26 EP 29 DI 10.5942/jawwa.2016.108.0180 PG 4 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA DU7YM UT WOS:000382430500005 ER PT J AU Kabilan, S Suffield, SR Recknagle, KP Jacob, RE Einstein, DR Kuprat, AP Carson, JP Colby, SM Saunders, JH Hines, SA Teeguarden, JG Straub, TM Moe, M Taft, SC Corley, RA AF Kabilan, S. Suffield, S. R. Recknagle, K. P. Jacob, R. E. Einstein, D. R. Kuprat, A. P. Carson, J. P. Colby, S. M. Saunders, J. H. Hines, S. A. Teeguarden, J. G. Straub, T. M. Moe, M. Taft, S. C. Corley, R. A. TI Computational fluid dynamics modeling of Bacillus anthracis spore deposition in rabbit and human respiratory airways SO JOURNAL OF AEROSOL SCIENCE LA English DT Article DE Three-dimensional computational fluid dynamics; Particle deposition; New Zealand white rabbit; Human Bacillus anthracis; Lung ID ZEALAND WHITE-RABBITS; INHALATIONAL ANTHRAX; PARTICLE DEPOSITION; HUMAN LUNGS; AIR-FLOW; AEROSOL DEPOSITION; INCUBATION PERIOD; RAT; DOSIMETRY; TRANSPORT AB Three-dimensional computational fluid dynamics and Lagrangian particle deposition models were developed to compare the deposition of aerosolized Bacillus anthracis spores in the respiratory airways of a human with that of the rabbit, a species commonly used in the study of anthrax disease. The respiratory airway geometries for each species were derived respectively from computed tomography (CT) and mu CT images. Both models encompassed airways that extended from the external nose to the lung with a total of 272 outlets in the human model and 2878 outlets in the rabbit model. All simulations of spore deposition were conducted under transient, inhalation-exhalation breathing conditions using average species-specific minute volumes. Two different exposure scenarios were modeled in the rabbit based upon experimental inhalation studies. For comparison, human simulations were conducted at the highest exposure concentration used during the rabbit experimental exposures. Results demonstrated that regional spore deposition patterns were sensitive to airway geometry and ventilation profiles. Due to the complex airway geometries in the rabbit nose, higher spore deposition efficiency was predicted in the nasal sinus compared to the human at the same air concentration of anthrax spores. In contrast, higher spore deposition was predicted in the lower conducting airways of the human compared to the rabbit lung due to differences in airway branching pattern. This information can be used to refine published and ongoing biokinetic models of inhalation anthrax spore exposures, which currently estimate deposited spore concentrations based solely upon exposure concentrations and inhaled doses that do not factor in species specific anatomy and physiology for deposition. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Kabilan, S.; Suffield, S. R.; Recknagle, K. P.; Jacob, R. E.; Einstein, D. R.; Kuprat, A. P.; Colby, S. M.; Teeguarden, J. G.; Straub, T. M.; Corley, R. A.] Pacific Northwest Natl Lab, 902 Battelle Blvd,POB 999,MSIN J4-16, Richland, WA 99352 USA. [Carson, J. P.] Texas Adv Comp Ctr, Austin, TX 78758 USA. [Saunders, J. H.; Hines, S. A.] Battelle Mem Inst, 505 King Ave, Columbus, OH 43201 USA. [Moe, M.] Dept Homeland Secur, Sci & Technol Directorate, Washington, DC 20528 USA. [Taft, S. C.] US EPA, Natl Homeland Secur Res Ctr, Threat & Consequence Assessment Div, Cincinnati, OH 45268 USA. RP Kabilan, S (reprint author), Pacific Northwest Natl Lab, 902 Battelle Blvd,POB 999,MSIN J4-16, Richland, WA 99352 USA. EM senthil.kabilan@pnnl.gov FU U.S. Environmental Protection Agency through its Office of Research and Development [EP-C-09-006, DW9792343401]; Department of Homeland Security, Science and Technology Directorate [HSHQPM-14-X-00037]; National Heart, Lung, and Blood Institute of the National Institutes of Health [R01 HL073598] FX The U.S. Environmental Protection Agency through its Office of Research and Development under Contract no. EP-C-09-006 and Interagency Agreement DW9792343401 and the Department of Homeland Security, Science and Technology Directorate through Contract HSHQPM-14-X-00037 funded and managed the research described here for the development of the rabbit model and for spore simulations in both species. It has been subjected to the EPA and DHS review and has been approved for publication. Note that approval does not signify that the contents necessarily reflect the views of EPA and DHS. Mention of trade names, products, or services does not convey official EPA/DHS approval, endorsement, or recommendation. Development of the human model was performed under a Grant from the National Heart, Lung, and Blood Institute of the National Institutes of Health (R01 HL073598). NR 53 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0021-8502 EI 1879-1964 J9 J AEROSOL SCI JI J. Aerosol. Sci. PD SEP PY 2016 VL 99 SI SI BP 64 EP 77 DI 10.1016/j.jaerosci.2016.01.011 PG 14 WC Engineering, Chemical; Engineering, Mechanical; Environmental Sciences; Meteorology & Atmospheric Sciences SC Engineering; Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DT6JE UT WOS:000381589700010 ER PT J AU de Villiers, TJ Hall, JE Pinkerton, JV Perez, SC Reese, M Yang, C Pierroz, DD AF de Villiers, T. J. Hall, J. E. Pinkerton, J. V. Perez, S. Cerdas Reese, M. Yang, C. Pierroz, D. D. TI Revised global consensus statement on menopausal hormone therapy SO MATURITAS LA English DT Article ID WOMEN AB The following Consensus Statement is endorsed by The International Menopause Society, The North American Menopause Society, The Endocrine Society, The European Menopause and Andropause Society, The Asia Pacific Menopause Federation, The International Osteoporosis Foundation and The Federation of Latin American Menopause Societies. (C) 2016 International Menopause Society and Elsevier Ireland Ltd. All rights reserved. C1 [de Villiers, T. J.] Univ Stellenbosch, Mediclin Panorama, ZA-7500 Cape Town, Parow, South Africa. [de Villiers, T. J.] Univ Stellenbosch, Dept Gynecol, ZA-7500 Cape Town, Parow, South Africa. [Hall, J. E.] NIH, Natl Inst Environm Hlth Sci, Res Triangle Pk, NC USA. [Pinkerton, J. V.] Univ Virginia Hlth Syst, Dept Obstet & Gynecol, Charlottesville, VA USA. [Perez, S. Cerdas] Hosp Cima, Dept Endocrinol, San Jose, Costa Rica. [Reese, M.] Univ Oxford, Oxford OX1 2JD, England. [Yang, C.] Mt Alvernia Hosp, Singapore, Singapore. [Pierroz, D. D.] Univ Geneva, CH-1211 Geneva 4, Switzerland. RP de Villiers, TJ (reprint author), Univ Stellenbosch, Mediclin Panorama, ZA-7500 Cape Town, Parow, South Africa.; de Villiers, TJ (reprint author), Univ Stellenbosch, Dept Gynecol, ZA-7500 Cape Town, Parow, South Africa. EM tobie@iafrica.com OI Hall, Janet/0000-0003-4644-3061 NR 6 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0378-5122 EI 1873-4111 J9 MATURITAS JI Maturitas PD SEP PY 2016 VL 91 BP 153 EP 155 DI 10.1016/j.maturitas.2016.06.001 PG 3 WC Geriatrics & Gerontology; Obstetrics & Gynecology SC Geriatrics & Gerontology; Obstetrics & Gynecology GA DT2QP UT WOS:000381325800021 PM 27389038 ER PT J AU White, HK Conmy, RN MacDonald, IR Reddy, CM AF White, Helen K. Conmy, Robyn N. MacDonald, Ian R. Reddy, Christopher M. TI Methods of Oil Detection in Response to the Deepwater Horizon Oil Spill SO OCEANOGRAPHY LA English DT Article ID GULF-OF-MEXICO; POLYCYCLIC AROMATIC-HYDROCARBONS; SYNTHETIC-APERTURE RADAR; FLOW-RATE; RADIOCARBON; SATELLITE; SEDIMENTS; BLOWOUT; IMPACT; COLUMN AB Detecting oil in the northern Gulf of Mexico following the Deepwater Horizon oil spill presented unique challenges due to the spatial and temporal extent of the spill and the subsequent dilution of oil in the environment. Over time, physical, chemical, and biological processes altered the composition of the oil, further complicating its detection. Reservoir fluid, containing gas and oil, released from the Macondo well was detected in surface and subsurface environments. Oil monitoring during and after the spill required a variety of technologies, including nimble adaptation of techniques developed for non-oil-related applications. The oil detection technologies employed varied in sensitivity, selectivity, strategy, cost, usability, expertise of user, and reliability. Innovative technologies ranging from remote sensing to laboratory analytical techniques were employed and produced new information relevant to oil spill detection, including the chemical characterization, the dispersion effectiveness, and the detection limits of oil. The challenge remains to transfer these new technologies to oil spill responders so that detection of oil following a spill can be improved. C1 [White, Helen K.] Haverford Coll, Chem, Haverford, PA 19041 USA. [Conmy, Robyn N.] US EPA, Cincinnati, OH 45268 USA. [MacDonald, Ian R.] Florida State Univ, Tallahassee, FL 32306 USA. [Reddy, Christopher M.] Woods Hole Oceanog Inst, Woods Hole, MA 02543 USA. RP White, HK (reprint author), Haverford Coll, Chem, Haverford, PA 19041 USA. EM hwhite@haverford.edu FU Gulf Research Program; NSF [OCE-1333148]; Gulf of Mexico Research Initiative; C-IMAGE consortium; DEEP-C consortium; RFP-II grant FX This research was made possible in part by grants from the Gulf Research Program (to HKW); NSF OCE-1333148 (to CMR); and the Gulf of Mexico Research Initiative supporting the ECOGIG-2 consortium (to IRM), the C-IMAGE consortium (to CMR), the DEEP-C consortium (to CMR), and an RFP-II grant (to HKW). The authors thank Jack Cook for the creation of Figure 1. This document has been subjected to peer review by the US Environmental Protection Agency, Office of Research and Development, and National Risk Management Research Laboratory, and approved for publication. Approval does not signify that the contents reflect the views of the agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 65 TC 2 Z9 2 U1 28 U2 28 PU OCEANOGRAPHY SOC PI ROCKVILLE PA P.O. BOX 1931, ROCKVILLE, MD USA SN 1042-8275 J9 OCEANOGRAPHY JI Oceanography PD SEP PY 2016 VL 29 IS 3 SI SI BP 76 EP 87 DI 10.5670/oceanog.2016.72 PG 12 WC Oceanography SC Oceanography GA DU6OM UT WOS:000382334500014 ER PT J AU Leung, MCK Hutson, MS Seifert, AW Spencer, RM Knudsen, TB AF Leung, Maxwell C. K. Hutson, M. Shane Seifert, Ashley W. Spencer, Richard M. Knudsen, Thomas B. TI Computational modeling and simulation of genital tubercle development SO REPRODUCTIVE TOXICOLOGY LA English DT Article; Proceedings Paper CT 44th Annual Conference of the European-Teratology-Society CY SEP 11-14, 2016 CL Dublin, IRELAND SP European Teratol Soc DE Agent-based model; Genital tubercle; Hypospadias; Computational toxicology ID TISSUE-SPECIFIC ROLES; EPITHELIAL-MESENCHYMAL TRANSFORMATION; REPRODUCTIVE-TRACT DEVELOPMENT; MATE GENITOURINARY SYSTEM; EXTERNAL GENITALIA; SONIC-HEDGEHOG; ANDROGEN RECEPTOR; ENDOCRINE DISRUPTION; URETHRAL DEVELOPMENT; DIVERSE MECHANISMS AB Hypospadias is a developmental defect of urethral tube closure that has a complex etiology involving genetic and environmental factors, including anti-androgenic and estrogenic disrupting chemicals; however, little is known about the morphoregulatory consequences of androgen/estrogen balance during genital tubercle (GT) development. Computer models that predictively model sexual dimorphism of the GT may provide a useful resource to translate chemical-target bipartite networks and their developmental consequences across the human-relevant chemical universe. Here, we describe a multicellular agent-based model of genital tubercle (GT) development that simulates urethrogenesis from the sexually-indifferent urethral plate stage to urethral tube closure. The prototype model, constructed in CompuCell3D, recapitulates key aspects of GT morphogenesis controlled by SHH, FGF10, and androgen pathways through modulation of stochastic cell behaviors, including differential adhesion, motility, proliferation, and apoptosis. Proper urethral tube closure in the model was shown to depend quantitatively on SHH- and FGF10-induced effects on mesenchymal proliferation and epithelial apoptosis-both ultimately linked to androgen signaling. In the absence of androgen, GT development was feminized and with partial androgen deficiency, the model resolved with incomplete urethral tube closure, thereby providing an in silico platform for probabilistic prediction of hypospadias risk across combinations of minor perturbations to the GT system at various stages of embryonic development. (C) 2016 Elsevier Inc. All rights reserved. C1 [Leung, Maxwell C. K.; Knudsen, Thomas B.] US EPA, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. [Leung, Maxwell C. K.; Hutson, M. Shane] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Hutson, M. Shane] Vanderbilt Univ, Vanderbilt Inst Integrat Biosyst Res & Educ, Dept Phys & Astron, Nashville, TN 37235 USA. [Seifert, Ashley W.] Univ Kentucky, Dept Biol, Lexington, KY 40506 USA. [Spencer, Richard M.] Lockheed Martin, Res Triangle Pk, NC 27709 USA. RP Leung, MCK; Knudsen, TB (reprint author), US EPA, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM leung.maxwell@epa.gov; knudsen.thomas@epa.gov NR 79 TC 0 Z9 0 U1 5 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0890-6238 J9 REPROD TOXICOL JI Reprod. Toxicol. PD SEP PY 2016 VL 64 SI SI BP 151 EP 161 DI 10.1016/j.reprotox.2016.05.005 PG 11 WC Reproductive Biology; Toxicology SC Reproductive Biology; Toxicology GA DU1GR UT WOS:000381956700013 PM 27180093 ER PT J AU Martinson, HM Bergmann, EJ Venugopal, PD Riley, CB Shrewsbury, PM Raupp, MJ AF Martinson, Holly M. Bergmann, Erik J. Venugopal, P. Dilip Riley, Christopher B. Shrewsbury, Paula M. Raupp, Michael J. TI Invasive stink bug favors naive plants: Testing the role of plant geographic origin in diverse, managed environments SO SCIENTIFIC REPORTS LA English DT Article ID HALYOMORPHA-HALYS; NORTH-AMERICA; INSECT HERBIVORES; BIOTIC RESISTANCE; ABUNDANCE; PENTATOMIDAE; ASSOCIATIONS; NURSERIES; IMPACTS; ECOLOGY AB With the introduction and establishment of exotic species, most ecosystems now contain both native and exotic plants and herbivores. Recent research identifies several factors that govern how specialist herbivores switch host plants upon introduction. Predicting the feeding ecology and impacts of introduced generalist species, however, remains difficult. Here, we examine how plant geographic origin, an indicator of shared co-evolutionary history, influences patterns of host use by a generalist, invasive herbivore, while accounting for variation in plant availability. The brown marmorated stink bug, Halyomorpha halys, is a highly polyphagous Asian herbivore and an economically important invasive pest in North America and Europe. In visual surveys of 220 plant taxa in commercial nurseries in Maryland, USA, H. halys was more abundant on non-Asian plants and selected these over Asian plants. The relationship between the relative use of plants and their availability was strongly positive but depended also on plant origin at two of our three sites, where the higher relative use of non-Asian plants was greatest for highly abundant taxa. These results highlight the importance of considering both plant origin and relative abundance in understanding the selection of host plants by invasive generalist herbivores in diverse, natural and urban forests. C1 [Martinson, Holly M.; Bergmann, Erik J.; Venugopal, P. Dilip; Shrewsbury, Paula M.; Raupp, Michael J.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Venugopal, P. Dilip] US EPA, Off Air & Radiat, Off Transportat & Air Qual, Washington, DC 20460 USA. [Riley, Christopher B.] Ohio State Univ, Dept Entomol, Columbus, OH 43210 USA. RP Martinson, HM (reprint author), Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. EM hmartinson@alumni.duke.edu FU USDA National Institute of Food and Agriculture (SCRI Award) [2011-51181-30937]; USDA National Institute of Food and Agriculture (McIntire Stennis Grant) [MD-ENTM-04168757] FX We are grateful to R. Wallace, C. Brodo, D. Reisinger, S. Harris, K. Beiter, C. McMullen, B. Cornwell, and K. Keochinda for field assistance, C. Sargent for logistical support, and S. Black, K. Lewis, and N. Graves for field access. This work was funded by grants from the USDA National Institute of Food and Agriculture (SCRI Award 2011-51181-30937, McIntire Stennis Grant MD-ENTM-04168757). NR 44 TC 0 Z9 0 U1 15 U2 16 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD SEP 1 PY 2016 VL 6 AR 32646 DI 10.1038/srep32646 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DU6OC UT WOS:000382333500001 PM 27581756 ER PT J AU Collette, TW Skelton, DM Davis, JM Cavallin, JE Jensen, KM Kahl, MD Villeneuve, DL Ankley, GT Martinovic-Weigelt, D Ekman, DR AF Collette, Timothy W. Skelton, David M. Davis, John M. Cavallin, Jenna E. Jensen, Kathleen M. Kahl, Michael D. Villeneuve, Daniel L. Ankley, Gerald T. Martinovic-Weigelt, Dalma Ekman, Drew R. TI Metabolite profiles of repeatedly sampled urine from male fathead minnows (Pimephales promelas) contain unique lipid signatures following exposure to anti-androgens SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS LA English DT Article DE Anti-androgens; Fathead minnows; Metabolite profiling; Metabolomics; Toxicogenomics ID ENDOCRINE-DISRUPTING CHEMICALS; MASS-SPECTROMETRY; BISPHENOL-A; GAS-CHROMATOGRAPHY; GENE-EXPRESSION; METABOLOMICS; FISH; RESPONSES; ANTIANDROGEN; PERSPECTIVES AB The purpose of this study was twofold. First, we sought to identify candidate markers of exposure to anti androgens by analyzing endogenous metabolite profiles in the urine of male fathead minnows (mFHM, Pimephales promelas). Based on earlier work, we hypothesized that unidentified lipids in the urine of mFHM were selectively responsive to exposure to androgen receptor antagonists, which is otherwise difficult to confirm using established fish toxicity assays. A second goal was to evaluate the feasibility of non-lethally and repeatedly sampling urine from individual mFHMs over the time course of response to a chemical exposure. Accordingly, we exposed mFHM to the model anti-androgens vinclozolin or flutamide. Urine was collected from each fish at 48 hour intervals over the course of a 14 day exposure. Parallel experiments were conducted with mFHM exposed to bisphenol A or control water. The frequent handling/sampling regime did not cause apparent adverse effects on the fish. Endogenous metabolite profiling was conducted with gas chromatography-mass spectrometry (GC-MS), which exhibited lower variation for the urinary metabolome than was found in earlier work with nuclear magnetic resonance (NMR) spectroscopy. Specifically, for inter-and intra-individual variations, the median spectrum-wide relative standard deviation (RSD) was 32.6% and 33.3%, respectively, for GC-MS analysis of urine from unexposed mFHM. These results compared favorably with similar measurements of urine from other model species, including the Sprague Dawley rat. In addition, GC-MS allowed us to identify several lipids (e.g., certain saturated fatty acids) in mFHM urine as candidate markers of exposure to androgen receptor antagonists. Published by Elsevier Inc. C1 [Collette, Timothy W.; Skelton, David M.; Davis, John M.; Ekman, Drew R.] US EPA, Natl Exposure Res Lab, 960 Coll Stn Rd, Athens, GA 30605 USA. [Cavallin, Jenna E.; Jensen, Kathleen M.; Kahl, Michael D.; Villeneuve, Daniel L.; Ankley, Gerald T.] US EPA, Natl Hlth & Environm Effects Res Lab, Duluth, MN 55804 USA. [Martinovic-Weigelt, Dalma] Univ St Thomas, 2115 Summit Ave, St Paul, MN 55105 USA. RP Collette, TW (reprint author), US EPA, Natl Exposure Res Lab, 960 Coll Stn Rd, Athens, GA 30605 USA. EM collette.tim@epa.gov FU Great Lakes National Program Office; appointment to the Postdoctoral Research Program at the National Exposure Research Laboratory; Oak Ridge Institute for Science and Education (ORISE) [DW8992298301]; ORISE fellowship FX J.M.D. was supported by the Great Lakes National Program Office and an appointment to the Postdoctoral Research Program at the National Exposure Research Laboratory, administered by the Oak Ridge Institute for Science and Education (ORISE) through interagency agreement (DW8992298301) between U.S. DOE and U.S. EPA. JEC was also supported by an ORISE fellowship on this agreement. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. Mention of trade names, products, or services does not convey, and should not be interpreted as conveying, official EPA approval, endorsement or recommendation. NR 49 TC 1 Z9 1 U1 8 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1744-117X EI 1878-0407 J9 COMP BIOCHEM PHYS D JI Comp. Biochem. Physiol. D-Genomics Proteomics PD SEP PY 2016 VL 19 BP 190 EP 198 DI 10.1016/j.cbd.2016.01.001 PG 9 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA DU7TA UT WOS:000382416300021 PM 26810197 ER PT J AU Andersen, CP King, G Plocher, M Storm, M Pokhrel, LR Johnson, MG Rygiewicz, PT AF Andersen, Christian P. King, George Plocher, Milt Storm, Marjorie Pokhrel, Lok R. Johnson, Mark G. Rygiewicz, Paul T. TI GERMINATION AND EARLY PLANT DEVELOPMENT OF TEN PLANT SPECIES EXPOSED TO TITANIUM DIOXIDE AND CERIUM OXIDE NANOPARTICLES SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Nanomaterials; Ecotoxicology; Plant toxicology; Germination; Plant development ID FUNCTIONALIZED SILVER NANOPARTICLES; COLLOIDAL STABILITY; TIO2 NANOPARTICLES; SURFACE-CHARGE; TOXICITY; PHYTOTOXICITY; ACCUMULATION; GENOTOXICITY; DISSOLUTION; SUSPENSIONS AB Ten agronomic plant species were exposed to different concentrations of nano-titanium dioxide (nTiO(2)) or nano-cerium oxide (nCeO(2)) (0 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL) to examine potential effects on germination and early seedling development. The authors modified a standard test protocol developed for soluble chemicals (OPPTS 850.4200) to determine if such an approach might be useful for screening engineered nanomaterials (ENMs) and whether there were differences in response across a range of commercially important plant species to 2 common metal oxide ENMs. Eight of 10 species responded to nTiO(2), and 5 species responded to nCeO(2). Overall, it appeared that early root growth may be a more sensitive indicator of potential effects from ENM exposure than germination. The observed effects did not always relate to the exposure concentration, indicating that mass-based concentration may not fully explain the developmental effects of these 2 ENMs. The results suggest that nTiO(2) and nCeO(2) have different effects on early plant growth of agronomic species, with unknown effects at later stages of the life cycle. In addition, standard germination tests, which are commonly used for toxicity screening of new materials, may not detect the subtle but potentially more important changes associated with early growth and development in terrestrial plants. Published 2016 Wiley Periodicals Inc. on behalf of SETAC. C1 [Andersen, Christian P.; Johnson, Mark G.; Rygiewicz, Paul T.] US EPA, Corvallis, OR 97333 USA. [King, George; Plocher, Milt; Storm, Marjorie] CSS Dynamac, Corvallis, OR USA. [Pokhrel, Lok R.] Temple Univ, Coll Publ Hlth, Philadelphia, PA 19122 USA. RP Andersen, CP (reprint author), US EPA, Corvallis, OR 97333 USA. EM andersen.christian@epa.gov FU US Environmental Protection Agency FX The present study was funded by the US Environmental Protection Agency. It has been subjected to the agency's peer and administrative review, and it has been approved for publication as a US Environmental Protection Agency document. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. The authors declare no competing financial interest. NR 31 TC 6 Z9 6 U1 16 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD SEP PY 2016 VL 35 IS 9 BP 2223 EP 2229 DI 10.1002/etc.3374 PG 7 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DU4MZ UT WOS:000382188500011 PM 26773270 ER PT J AU Beyer, WN Basta, NT Chaney, RL Henry, PFP Mosby, DE Rattner, BA Scheckel, KG Sprague, DT Weber, JS AF Beyer, W. Nelson Basta, Nicholas T. Chaney, Rufus L. Henry, Paula F. P. Mosby, David E. Rattner, Barnett A. Scheckel, Kirk G. Sprague, Daniel T. Weber, John S. TI BIOACCESSIBILITY TESTS ACCURATELY ESTIMATE BIOAVAILABILITY OF LEAD TO QUAIL SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Metal bioavailability; Ecological risk assessment; Soil contamination; Wildlife toxicology ID RELATIVE BIOAVAILABILITY; CONTAMINATED SEDIMENT; RISK-ASSESSMENT; SOIL; CADMIUM; PHOSPHATE; ABSORPTION; EXTRACTION; SPECIATION; WATERFOWL AB Hazards of soil-borne lead (Pb) to wild birds may be more accurately quantified if the bioavailability of that Pb is known. To better understand the bioavailability of Pb to birds, the authors measured blood Pb concentrations in Japanese quail (Coturnix japonica) fed diets containing Pb-contaminated soils. Relative bioavailabilities were expressed by comparison with blood Pb concentrations in quail fed a Pb acetate reference diet. Diets containing soil from 5 Pb-contaminated Superfund sites had relative bioavailabilities from 33% to 63%, with a mean of approximately 50%. Treatment of 2 of the soils with phosphorus (P) significantly reduced the bioavailability of Pb. Bioaccessibility of Pb in the test soils was then measured in 6 in vitro tests and regressed on bioavailability: the relative bioavailability leaching procedure at pH 1.5, the same test conducted at pH 2.5, the Ohio State University in vitro gastrointestinal method, the urban soil bioaccessible lead test, the modified physiologically based extraction test, and the waterfowl physiologically based extraction test. All regressions had positive slopes. Based on criteria of slope and coefficient of determination, the relative bioavailability leaching procedure at pH 2.5 and Ohio State University in vitro gastrointestinal tests performed very well. Speciation by X-ray absorption spectroscopy demonstrated that, on average, most of the Pb in the sampled soils was sorbed to minerals (30%), bound to organic matter (24%), or present as Pb sulfate (18%). Additional Pb was associated with P (chloropyromorphite, hydroxypyromorphite, and tertiary Pb phosphate) and with Pb carbonates, leadhillite (a lead sulfate carbonate hydroxide), and Pb sulfide. The formation of chloropyromorphite reduced the bioavailability of Pb, and the amendment of Pb-contaminated soils with P may be a thermodynamically favored means to sequester Pb. Published (C) 2016 Wiley Periodicals Inc. on behalf of SETAC. C1 [Beyer, W. Nelson; Henry, Paula F. P.; Rattner, Barnett A.; Sprague, Daniel T.] US Geol Survey, Patuxent Wildlife Res Ctr, Beltsville, MD 20705 USA. [Basta, Nicholas T.] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. [Chaney, Rufus L.] USDA, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Mosby, David E.; Weber, John S.] US Fish & Wildlife Serv, Columbia, MO USA. [Scheckel, Kirk G.] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. RP Beyer, WN (reprint author), US Geol Survey, Patuxent Wildlife Res Ctr, Beltsville, MD 20705 USA. EM nbeyer@usgs.gov OI Scheckel, Kirk/0000-0001-9326-9241 FU Department of Energy; Office of Science by Argonne National Laboratory [DE-AC02-06CH11357] FX We thank K. Nelson (Helena, MT) and B. Spears (Fairhope, AL) of the US Fish and Wildlife Service for sending some of the soils used in the present study. Materials Research Collaborative Access Team operations are supported by the Department of Energy and member institutions. The present study used resources of the Advanced Photon Source, a US Department of Energy Office of Science User Facility operated for the Office of Science by Argonne National Laboratory under contract DE-AC02-06CH11357. NR 44 TC 1 Z9 1 U1 20 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD SEP PY 2016 VL 35 IS 9 BP 2311 EP 2319 DI 10.1002/etc.3399 PG 9 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DU4MZ UT WOS:000382188500022 PM 26876015 ER PT J AU Brill, JL Belanger, SE Chaney, JG Dyer, SD Raimondo, S Barron, MG Pittinger, CA AF Brill, Jessica L. Belanger, Scott E. Chaney, Joel G. Dyer, Scott D. Raimondo, Sandy Barron, Mace G. Pittinger, Charles A. TI DEVELOPMENT OF ALGAL INTERSPECIES CORRELATION ESTIMATION MODELS FOR CHEMICAL HAZARD ASSESSMENT SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Algae; Aquatic toxicology; Hazard/ risk assessment; Web-based Interspecies Correlation Estimation ID SPECIES SENSITIVITY DISTRIBUTIONS; ECOLOGICAL RISK-ASSESSMENT; WATER-QUALITY CRITERIA; TOXICITY; FISH; SUBSTANCES; STRATEGY; REDUCE AB Web-based Interspecies Correlation Estimation (ICE) is an application developed to predict the acute toxicity of a chemical from 1 species to another taxon. Web-ICE models use the acute toxicity value for a surrogate species to predict effect values for other species, thus potentially filling in data gaps for a variety of environmental assessment purposes. Web-ICE has historically been dominated by aquatic and terrestrial animal prediction models. Web-ICE models for algal species were essentially absent and are addressed in the present study. Acompilation of public and private sector-held algal toxicity datawere compiled and reviewed for quality based on relevant aspects of individual studies. Interspecies correlations were constructed from the most commonly tested algal genera for a broad spectrum of chemicals. The ICE regressions were developed based on acute 72-h and 96-h endpoint values involving 1647 unique studies on 476 unique chemicals encompassing 40 genera and 70 species of green, blue-green, and diatom algae. Acceptance criteria for algal ICE models were established prior to evaluation of individual models and included a minimum sample size of 3, a statistically significant regression slope, and a slope estimation parameter 0.65. A total of 186 ICE models were possible at the genus level, with 21 meeting quality criteria; and 264 ICE models were developed at the species level, with 32 meeting quality criteria. Algal ICE models will have broad utility in screening environmental hazard assessments, data gap filling in certain regulatory scenarios, and as supplemental information to derive species sensitivity distributions. Published 2016 Wiley Periodicals Inc. on behalf of SETAC. C1 [Brill, Jessica L.; Belanger, Scott E.; Dyer, Scott D.] Procter & Gamble Co, Mason Business Ctr, Environm Stewardship & Sustainabil, Cincinnati, OH 45202 USA. [Chaney, Joel G.] Procter & Gamble Co, Mason Business Ctr, Global Stat & Data Management, Cincinnati, OH USA. [Raimondo, Sandy; Barron, Mace G.] US EPA, Gulf Ecol Div, Natl Hlth & Environm Effects Lab, Gulf Breeze, FL USA. [Pittinger, Charles A.] Toxicol Excellence Risk Assessment, Cincinnati, OH USA. RP Brill, JL (reprint author), Procter & Gamble Co, Mason Business Ctr, Environm Stewardship & Sustainabil, Cincinnati, OH 45202 USA. EM brilll.jl@pg.com OI Belanger, Scott/0000-0003-0369-9673 NR 51 TC 0 Z9 0 U1 7 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD SEP PY 2016 VL 35 IS 9 BP 2368 EP 2378 DI 10.1002/etc.3375 PG 11 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DU4MZ UT WOS:000382188500028 PM 26792236 ER PT J AU Fulk, F Haynes, EN Hilbert, TJ Brown, D Petersen, D Reponen, T AF Fulk, Florence Haynes, Erin N. Hilbert, Timothy J. Brown, David Petersen, Dan Reponen, Tiina TI Comparison of stationary and personal air sampling with an air dispersion model for children's ambient exposure to manganese SO JOURNAL OF EXPOSURE SCIENCE AND ENVIRONMENTAL EPIDEMIOLOGY LA English DT Article DE AERMOD; ambient; children; manganese; modeling; sampling ID BLOOD LEAD LEVELS; FERROMANGANESE REFINERY; AIRBORNE MANGANESE; MEXICAN CHILDREN; YOUNG-CHILDREN; OHIO; NEUROTOXICITY; COMMUNITY; DUST; RISK AB Manganese (Mn) is ubiquitous in the environment and essential for normal growth and development, yet excessive exposure can lead to impairments in neurological function. This study modeled ambient Mn concentrations as an alternative to stationary and personal air sampling to assess exposure for children enrolled in the Communities Actively Researching Exposure Study in Marietta, OH. Ambient air Mn concentration values were modeled using US Environmental Protection Agency's Air Dispersion Model AERMOD based on emissions from the ferromanganese refinery located in Marietta. Modeled Mn concentrations were compared with Mn concentrations from a nearby stationary air monitor. The Index of Agreement for modeled versus monitored data was 0.34 (48 h levels) and 0.79 (monthly levels). Fractional bias was 0.026 for 48 h levels and -0.019 for monthly levels. The ratio of modeled ambient air Mn to measured ambient air Mn at the annual time scale was 0.94. Modeled values were also time matched to personal air samples for 19 children. The modeled values explained a greater degree of variability in personal exposures compared with time-weighted distance from the emission source. Based on these results modeled Mn concentrations provided a suitable approach for assessing airborne Mn exposure in this cohort. C1 [Fulk, Florence] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [Haynes, Erin N.; Hilbert, Timothy J.; Reponen, Tiina] Univ Cincinnati, Coll Med, Dept Environm Hlth, 160 Panzeca Way, Cincinnati, OH 45267 USA. [Brown, David] Marietta Coll, Marietta, OH USA. [Petersen, Dan] US Environm Protect Age, Natl Ctr Environm Assessment, Cincinnati, OH USA. RP Haynes, EN (reprint author), Univ Cincinnati, Coll Med, Dept Environm Hlth, 160 Panzeca Way, Cincinnati, OH 45267 USA. EM Erin.Haynes@uc.edu FU National Institute of Environmental Health Sciences [1R01 ES016531]; NIEHS [1R01 ES016531, P30ES006096] FX We acknowledge Joshua Mickle, Derek Hennen, Russellitta Young, Pierce Kuhnell, Jody Alden, Mary Barnas, Dawn Wittberg, James Thurman, and the CARES Advisory Board. The views expressed in this paper are those of the authors and do not necessarily represent the views or policies of the US Environmental Protection Agency. This work was supported by funding from National Institute of Environmental Health Sciences (1R01 ES016531) and NIEHS (1R01 ES016531 and P30ES006096). This work was completed in partial fulfillment of the Doctor of Philosophy degree in Epidemiology in the Department of Environmental Health, Division of Epidemiology, University of Cincinnati College of Medicine. NR 53 TC 0 Z9 0 U1 8 U2 8 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1559-0631 EI 1559-064X J9 J EXPO SCI ENV EPID JI J. Expo. Sci. Environ. Epidemiol. PD SEP-OCT PY 2016 VL 26 IS 5 BP 494 EP 502 DI 10.1038/jes.2016.30 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DT8YB UT WOS:000381780500009 PM 27168393 ER PT J AU Xu, XH Liu, N Landis, MS Feng, XB Qiu, GL AF Xu, Xiaohang Liu, Na Landis, Matthew S. Feng, Xinbin Qiu, Guangle TI Characteristics and distributions of atmospheric mercury emitted from anthropogenic sources in Guiyang, southwestern China SO ACTA GEOCHIMICA LA English DT Article DE Atmospheric mercury; Speciation; Anthropogenic sources; GEM; PHg; RGM ID TOTAL GASEOUS MERCURY; FIRED POWER-PLANTS; AMBIENT AIR; SPECIATED MERCURY; PARTICULATE MERCURY; EMISSION SOURCES; TRANSPORT; CITY; COAL; SHANGHAI AB Continuous measurements of speciated atmospheric mercury (Hg), including gaseous elemental mercury (GEM), particulate mercury (PHg), and reactive gaseous mercury (RGM) were conducted in Guizhou Province, southwestern China. Guiyang Power Plant (GPP), Guiyang Wujiang Cement Plant, Guizhou Aluminum Plant (GAP), and Guiyang Forest Park (GFP) in Guiyang were selected as study sites. Automatic Atmospheric Mercury Speciation Analyzers (Tekran 2537A) were used for GEM analysis. PHg and RGM were simultaneously collected by a manual sampling system, including elutriator, coupler/impactor, KCl-coated annular denuder, and a filter holder. Results show that different emission sources dominate different species of Hg. The highest average GEM value was 22.2 +/- 28.3 ng.m(-3) and the lowest 6.1 +/- 3.9 ng.m(-3), from samples collected at GPP and GAP, respectively. The maximum average PHg was 1984.9 pg.m(-3) and the minimum average 55.9 pg.m(-3), also from GPP and GAP, respectively. Similarly, the highest average RGM of 68.8 pg.m(-3) was measured at GPP, and the lowest level of 20.5 pg.m(-3) was found at GAP. We conclude that coal combustion sources are still playing a key role in GEM; traffic contributes significantly to PHg; and domestic pollution dominates RGM. C1 [Xu, Xiaohang; Liu, Na; Feng, Xinbin; Qiu, Guangle] Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550081, Peoples R China. [Xu, Xiaohang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China. [Landis, Matthew S.] US EPA, Off Res & Dev, Durham, NC 27709 USA. RP Qiu, GL (reprint author), Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550081, Peoples R China. EM qiuguangle@vip.skleg.cn NR 62 TC 0 Z9 0 U1 9 U2 12 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 2096-0956 EI 2365-7499 J9 ACTA GEOCHIM JI ACTA GEOCHIM. PD SEP PY 2016 VL 35 IS 3 BP 240 EP 250 DI 10.1007/s11631-016-0111-9 PG 11 WC Geochemistry & Geophysics SC Geochemistry & Geophysics GA DT1DX UT WOS:000381223000004 ER PT J AU Chen, HH Williams, D Walker, JT Shi, W AF Chen, Huaihai Williams, David Walker, John T. Shi, Wei TI Probing the biological sources of soil N2O emissions by quantum cascade laser-based N-15 isotopocule analysis SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Nitrous oxide; Isotopocule; Isotopic site-preference; Quantum cascade laser spectrometer ID RATIO MASS-SPECTROMETRY; NITROUS-OXIDE; FUNGAL DENITRIFICATION; NITRIFICATION; ISOTOPOMERS; SIGNATURES; FRACTIONATION; SPECTROSCOPY; ECOSYSTEMS; MOISTURE AB Isotopocule analysis by quantum cascade laser spectrometry (QCL) is a promising approach for in situ, real-time tracking of the biological sources of soil N2O emissions. However, background atmospheric N2O is an important source of variability in the measurement of isotopocule ratios (i.e.,(NNO)-N-14-N-15-O-16/(NNO)-N-14-N-14-O-16 and (NNO)-N-15-N-14-O-16/(NNO)-N-14-N-14-O-16) of gas samples. Here, a method based on Keeling plot for determining the intramolecular N-15 distribution in N2O is introduced. The sensitivity and reliability of this method are examined against N2O of known N-15 site preference (SP), and N2O produced from fungal and bacterial isolates, soils with different moisture contents and organic amendments, and a soil chamber under field conditions. The isotopocules of N2O determined by standard gases supported that the Keeling plot method was more reliable than the averaging method. Using this method, SP of N2O was greater in fungal than bacterial denitrifiers, as well as in soil at 60% water filled pore space (WFPS) than 80-100% WFPS. This method also determined that the SP of N2O was distinct between soils of different substrate complexity. Further, we observed a N2O SP between - 18.9 parts per thousand and 2.2% in a soil flux chamber deployed in a corn field after 2 d of rainfalls that is consistent with the SP of N2O produced from bacterial denitrification and nitrifier denitrification. Our data demonstrate that this Keeling plot method provides accurate discrimination of biological sources when N2O is analyzed by the QCL system. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Chen, Huaihai; Shi, Wei] North Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Chen, Huaihai; Williams, David] US EPA, Div Environm Sci, Natl Exposure Res Lab, Res Triangle Pk, NC USA. [Walker, John T.] US EPA, Air Pollut Prevent & Control Div, Natl Risk Management Res Lab, Res Triangle Pk, NC USA. RP Shi, W (reprint author), North Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. EM wei_shi@ncsu.edu RI Chen, Huaihai/G-4462-2015 OI Chen, Huaihai/0000-0002-2121-1822 FU USDA-NIFA [2011-67019-30189]; US-EPA ORD FX This study was financially supported by USDA-NIFA #2011-67019-30189 and US-EPA ORD. We thank Dr. Joachim Mohn from EMPA for QCL calibration. Our sincere appreciation also goes to two reviewers for their constructive comments and suggestions. Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy. NR 29 TC 2 Z9 2 U1 24 U2 28 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD SEP PY 2016 VL 100 BP 175 EP 181 DI 10.1016/j.soilbio.2016.06.015 PG 7 WC Soil Science SC Agriculture GA DS2LD UT WOS:000380600100020 ER PT J AU Hanson, A Johnson, R Wigand, C Oczkowski, A Davey, E Markham, E AF Hanson, Alana Johnson, Roxanne Wigand, Cathleen Oczkowski, Autumn Davey, Earl Markham, Erin TI Responses of Spartina alterniflora to Multiple Stressors: Changing Precipitation Patterns, Accelerated Sea Level Rise, and Nutrient Enrichment SO ESTUARIES AND COASTS LA English DT Article DE Salt marsh; Spartina alterniflora; Climate change; Precipitation; Sea level rise; Eutrophication ID SALT-MARSH; CLIMATE-CHANGE; NEW-ENGLAND; EUTROPHICATION; BIODIVERSITY; VEGETATION; DYNAMICS; LOADS AB Coastal wetlands, well recognized for their ecosystem services, have faced many threats throughout the USA and elsewhere. While managers require good information on the net impact of these combined stressors on wetlands, little such information exists. We conducted a 4-month mesocosm study to analyze the multiple stressor effects of precipitation changes, sea level rise, and eutrophication on the salt marsh plant Spartina alterniflora. Pots containing plants in an organic soil matrix were positioned in tanks and received Narragansett Bay (RI, USA) water. The study simulated three precipitation levels (ambient daily rain, biweekly storm, and drought), three levels of tidal inundations (high (15 cm below mean high water (MHW)), mean (MHW), and low (15 cm above MHW)), and two nutrient enrichment levels (unenriched and nutrient-enriched bay water). Our results demonstrate that storm and drought stressors led to significantly less above- and belowground biomass than those in ambient rain conditions. Plants that were flooded at high inundation had less belowground biomass, fine roots, and shoots. Nutrients had no detectable effect on aboveground biomass, but the enriched pots had higher stem counts and more fine roots than unenriched pots, in addition to greater CO2 emission rates; however, the unenriched pots had significantly more coarse roots and rhizomes, which help to build peat in organogenic marshes. These results suggest that multiple stressors of altered precipitation, sea level rise, and nutrient enrichment would lead to reduced marsh sustainability. C1 [Hanson, Alana; Johnson, Roxanne; Wigand, Cathleen; Oczkowski, Autumn; Davey, Earl] US EPA, Atlantic Ecol Div, 27 Tarzwell Dr, Narragansett, RI 02882 USA. [Markham, Erin] Univ Rhode Isl, Grad Sch Oceanog, Narragansett, RI 02882 USA. RP Hanson, A (reprint author), US EPA, Atlantic Ecol Div, 27 Tarzwell Dr, Narragansett, RI 02882 USA. EM hanson.alana@epa.gov FU U.S. Environmental Protection Agency FX We thank Beth Watson, Kristen Jones, Joe Bishop, Gabrielle Sousa, and Kirk Silver for field and laboratory assistance; Kevin Kelly, John Sardelli, Bob Dow, Adam Kopacsi, and Russ Ahlgren for maintenance and construction of tanks and mesocosms; Kenneth Miller for statistical analysis; and Patricia DeCastro for graphical arts support. Sandi Robinson, Rick McKinney, and Giancarlo Cicchetti provided helpful input on an earlier version of the manuscript. This is ORD tracking number ORD-010361 of the U.S. EPA's Office of Research and Development, National Health and Environmental Effects Research Laboratory, Atlantic Ecology Division. Although the information in this document has been funded by the U.S. Environmental Protection Agency, it does not necessarily reflect the views of the agency and no official endorsement should be inferred. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 39 TC 1 Z9 1 U1 43 U2 59 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1559-2723 EI 1559-2731 J9 ESTUAR COAST JI Estuaries Coasts PD SEP PY 2016 VL 39 IS 5 BP 1376 EP 1385 DI 10.1007/s12237-016-0090-4 PG 10 WC Environmental Sciences; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DS0EO UT WOS:000380268400006 ER PT J AU Liu, XY Allen, MR Roache, NF AF Liu, Xiaoyu Allen, Matthew R. Roache, Nancy F. TI Characterization of organophosphorus flame retardants' sorption on building materials and consumer products SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Organophosphorus flame retardants; Material-air partition coefficient; Material-phase diffusion coefficient; Sink; Sorption strength ID SEMIVOLATILE ORGANIC-COMPOUNDS; WASTE-WATER; INDOOR ENVIRONMENTS; HISTORY METHOD; DUST SAMPLES; PLASTICIZERS; EMISSION; CONTAMINANTS; EXPOSURE; PARAMETERS AB Better understanding the transport mechanisms of organophosphorus flame-retardants (OPFRs) in the residential environment is important to more accurately estimate their indoor exposure and develop risk management strategies that protect human health. This study describes an improved dual small chamber testing method to characterize the sorption of OPFRs on indoor building materials and consumer products. The OPFRs studied were tris(2-chloroethyl) phosphate (TCEP), tris(1-chloro-2-propyl) phosphate (TCIPP), and tris(1,3-dichloro-2-propyl) phosphate (TDCIPP). The test materials and products used as sinks include concrete, ceiling tile, vinyl flooring, carpet, latex painted gypsum wallboard, open cell polyurethane foam, mattress pad and liner, polyester clothing, cotton clothing, and uniform shirt. During the tests, the amount of OPFRs absorbed by the materials at different exposure times was determined simultaneously. OPFRs air concentrations at the inlet and inside the test chamber were monitored. The data were used to rank the sorption strength of the OPFRs on different materials. In general, building materials exhibited relatively stronger sorption strength than clothing textiles. The material-air partition and material phase diffusion coefficients were estimated by fitting a sink model to the sorption concentration data for twelve materials with three OPFRs. They are in the range of 2.72 x 10(5) to 3.99 x 10(5) (dimensionless) for the material-air partition coefficients and 1.13 x 10(-14) to 5.83 x 10(-9) (m(2)/h) for the material phase diffusion coefficients. Published by Elsevier Ltd. C1 [Liu, Xiaoyu] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Allen, Matthew R.] Jacobs Technol Inc, 600 William Northern Blvd, Tullahoma, TN 37388 USA. [Roache, Nancy F.] ARCADIS US Inc, 4915 Prospectus Dr,Suite F, Durham, NC 27713 USA. RP Liu, XY (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. EM liu.xiaoyu@epa.gov NR 52 TC 0 Z9 0 U1 23 U2 41 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD SEP PY 2016 VL 140 BP 333 EP 341 DI 10.1016/j.atmosenv.2016.06.019 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DR7MG UT WOS:000380083200029 ER PT J AU Baker, KR Woody, MC Tonnesen, GS Hutzell, W Pye, HOT Beaver, MR Pouliot, G Pierce, T AF Baker, K. R. Woody, M. C. Tonnesen, G. S. Hutzell, W. Pye, H. O. T. Beaver, M. R. Pouliot, G. Pierce, T. TI Contribution of regional-scale fire events to ozone and PM2.5 air quality estimated by photochemical modeling approaches SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Wild fire; Prescribed fire; Photochemical model; Ozone; Particulate matter ID ORGANIC AEROSOL EVOLUTION; BIOMASS BURNING AEROSOLS; LIGHT-ABSORPTION; WILDFIRE PLUMES; BROWN CARBON; SMOKE PLUMES; WESTERN US; NOX; SENSITIVITY; OXIDATION AB Two specific fires from 2011 are tracked for local to regional scale contribution to ozone (O-3) and fine particulate matter (PM2.5) using a freely available regulatory modeling system that includes the BlueSky wildland fire emissions tool, Spare Matrix Operator Kernel Emissions (SMOKE) model, Weather and Research Forecasting (WRF) meteorological model, and Community Multiscale Air Quality (CMAQ) photochemical grid model. The modeling system was applied to track the contribution from a wildfire (Wallow) and prescribed fire (Flint Hills) using both source sensitivity and source apportionment approaches. The model estimated fire contribution to primary and secondary pollutants are comparable using source sensitivity (brute-force zero out) and source apportionment (Integrated Source Apportionment Method) approaches. Model estimated O-3 enhancement relative to CO is similar to values reported in literature indicating the modeling system captures the range of O-3 inhibition possible near fires and O-3 production both near the fire and downwind. O-3 and peroxyacetyl nitrate (PAN) are formed in the fire plume and transported downwind along with highly reactive VOC species such as formaldehyde and acetaldehyde that are both emitted by the fire and rapidly produced in the fire plume by VOC oxidation reactions. PAN and aldehydes contribute to continued downwind O-3 production. The transport and thermal decomposition of PAN to nitrogen oxides (NOx) enables O-3 production in areas limited by NOx availability and the photolysis of aldehydes to produce free radicals (HOX) causes increased O-3 production in NOx rich areas. The modeling system tends to overestimate hourly surface O-3 at routine rural monitors in close proximity to the fires when the model predicts elevated fire impacts on O-3 and Hazard Mapping System (HMS) data indicates possible fire impact. A sensitivity simulation in which solar radiation and photolysis rates were more aggressively attenuated by aerosol in the plume reduced model O-3 but does not eliminate this bias. A comparison of model predicted daily average speciated PM2.5 at surface rural routine network sites when the model predicts fire impacts from either of these fires shows a tendency toward overestimation of PM2.5 organic aerosol in close proximity to these fires. The standard version of the CMAQtreats primarily emitted organic aerosol as non-volatile. An alternative approach for treating organic aerosol as semi-volatile resulted in lower PM2.5 organic aerosol from these fires but does not eliminate the bias. Future work should focus on modeling specific fire events that are well characterized in terms of size, emissions, and have extensive measurements taken near the fire and downwind to better constrain model representation of important physical and chemical processes (e.g. aerosol photolysis attenuation and organic aerosol treatment) related to wild and prescribed fires. Published by Elsevier Ltd. C1 [Baker, K. R.; Beaver, M. R.] US EPA, Off Air Qual Planning & Stand, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Woody, M. C.; Hutzell, W.; Pye, H. O. T.; Pouliot, G.; Pierce, T.] US EPA, Off Res & Dev, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Tonnesen, G. S.] US EPA, Reg 8,1595 Wynkoop St, Denver, CO 80202 USA. RP Baker, KR (reprint author), US EPA, Off Air Qual Planning & Stand, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM baker.kirk@epa.gov RI Pye, Havala/F-5392-2012 OI Pye, Havala/0000-0002-2014-2140 NR 75 TC 1 Z9 1 U1 42 U2 57 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD SEP PY 2016 VL 140 BP 539 EP 554 DI 10.1016/j.atmosenv.2016.06.032 PG 16 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DR7MG UT WOS:000380083200050 ER PT J AU Kenyon, EM Eklund, C Leavens, T Pegram, RA AF Kenyon, Elaina M. Eklund, Christopher Leavens, Teresa Pegram, Rex A. TI Development and application of a human PBPK model for bromodichloromethane to investigate the impacts of multi-route exposure SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE bromodichloromethane; multi-route exposure; PBPK model ID DISINFECTION BY-PRODUCTS; BLOOD TRIHALOMETHANE LEVELS; DRINKING-WATER SOURCE; PHARMACOKINETIC MODELS; TAP WATER; BLADDER-CANCER; PARTITION-COEFFICIENTS; SENSITIVITY-ANALYSIS; BIOMONITORING DATA; DRUG CONCENTRATION AB As a result of its presence in water as a volatile disinfection byproduct, bromodichloromethane (BDCM), which is mutagenic, poses a potential health risk from exposure via oral, dermal and inhalation routes. We developed a refined human physiologically based pharmacokinetic (PBPK) model for BDCM (including new chemical-specific human parameters) to evaluate the impact of BDCM exposure during showering and bathing on important measures of internal dose compared with oral exposure. The refined model adequately predicted data from the published literature for oral, dermal and bathing/showering exposures. A liter equivalency approach (L-eq) was used to estimate BDCM concentration in a liter of water consumed by the oral route that would be required to produce the same internal dose of BDCM resulting from a 20-min bath or a 10-min shower in water containing 10 mu gl(-1) BDCM. The oral liter equivalent concentrations for the bathing scenario were 605, 803 and 5 mu gl(-1) BDCM for maximum venous blood concentration (Cmax), the area under the curve (AUCv) and the amount metabolized in the liver per hour (MBDCM), respectively. For a 10-min showering exposure, the oral L-eq concentrations were 282, 312 and 2.1 mu gl(-1) for Cmax, AUC and MBDCM, respectively. These results demonstrate large contributions of dermal and inhalation exposure routes to the internal dose of parent chemical reaching the systemic circulation, which could be transformed to mutagenic metabolites in extrahepatic target tissues. Thus, consideration of the contribution of multiple routes of exposure when evaluating risks from water-borne BDCM is needed, and this refined human model will facilitate improved assessment of internal doses from real-world exposures. Published 2015. This article has been contributed to by US Government employees and their work is in the public domain in the USA. A refined human physiologically based pharmacokinetic (PBPK) model for bromodichloromethane (BDCM) (including new chemical-specific human parameters) was developed to evaluate the impact of BDCM exposure during showering and bathing on important measures of internal dose compared with oral exposure. Analyses demonstrated large contributions of dermal and inhalation exposure routes to an internal dose of the parent chemical reaching the systemic circulation. Thus, consideration of the contribution of multiple routes of exposure when evaluating risks from water-borne BDCM is highly desirable. C1 [Kenyon, Elaina M.; Eklund, Christopher; Pegram, Rex A.] US EPA, Integrated Syst Toxicol Div, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. RP Kenyon, EM (reprint author), US EPA, NHEERL ISTD PB Mail Drop B105-03, Res Triangle Pk, NC 27711 USA. EM kenyon.elaina@epa.gov NR 69 TC 1 Z9 1 U1 7 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD SEP PY 2016 VL 36 IS 9 BP 1095 EP 1111 DI 10.1002/jat.3269 PG 17 WC Toxicology SC Toxicology GA DR5PD UT WOS:000379954000003 PM 26649444 ER PT J AU Strickland, J Zang, QD Kleinstreuer, N Paris, M Lehmann, DM Choksi, N Matheson, J Jacobs, A Lowit, A Allen, D Casey, W AF Strickland, Judy Zang, Qingda Kleinstreuer, Nicole Paris, Michael Lehmann, David M. Choksi, Neepa Matheson, Joanna Jacobs, Abigail Lowit, Anna Allen, David Casey, Warren TI Integrated decision strategies for skin sensitization hazard SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE skin sensitization; allergic contact dermatitis; integrated decision strategy; machine learning; LLNA; DPRA; KeratinoSens; h-CLAT; support vector machine ID LYMPH-NODE ASSAY; LINE ACTIVATION TEST; TEST H-CLAT; SCREENING CONTACT ALLERGENS; PEPTIDE REACTIVITY ASSAY; NONANIMAL TEST METHODS; PIG MAXIMIZATION TEST; RISK-ASSESSMENT MODEL; IN-VITRO METHODS; PREDICTIVE PERFORMANCE AB One of the top priorities of the Interagency Coordinating Committee for the Validation of Alternative Methods (ICCVAM) is the identification and evaluation of non-animal alternatives for skin sensitization testing. Although skin sensitization is a complex process, the key biological events of the process have been well characterized in an adverse outcome pathway (AOP) proposed by the Organisation for Economic Co-operation and Development (OECD). Accordingly, ICCVAM is working to develop integrated decision strategies based on the AOP using in vitro, in chemico and in silico information. Data were compiled for 120 substances tested in the murine local lymph node assay (LLNA), direct peptide reactivity assay (DPRA), human cell line activation test (h-CLAT) and KeratinoSens assay. Data for six physicochemical properties, which may affect skin penetration, were also collected, and skin sensitization read-across predictions were performed using OECD QSAR Toolbox. All data were combined into a variety of potential integrated decision strategies to predict LLNA outcomes using a training set of 94 substances and an external test set of 26 substances. Fifty-four models were built using multiple combinations of machine learning approaches and predictor variables. The seven models with the highest accuracy (89-96% for the test set and 96-99% for the training set) for predicting LLNA outcomes used a support vector machine (SVM) approach with different combinations of predictor variables. The performance statistics of the SVM models were higher than any of the non-animal tests alone and higher than simple test battery approaches using these methods. These data suggest that computational approaches are promising tools to effectively integrate data sources to identify potential skin sensitizers without animal testing. Published 2016. This article has been contributed to by US Government employees and their work is in the public domain in the USA. The Interagency Coordinating Committee for the Validation of Alternative Methods (ICCVAM) evaluated a non-animal decision strategies to predict skin sensitization. Machine learning approaches integrated in vitro, in chemico and in silico data and six physicochemical properties for 120 substances to predict murine local lymph node assay (LLNA) outcomes. The seven models with the highest accuracy used a support vector machine with different combinations of predictor variables. The models outperformed individual non-animal methods and test batteries. This suggests that computational approaches are promising tools to effectively integrate data to identify potential skin sensitizers without animal testing. C1 [Strickland, Judy; Zang, Qingda; Kleinstreuer, Nicole; Paris, Michael; Choksi, Neepa; Allen, David] ILS, Res Triangle Pk, NC 27709 USA. [Lehmann, David M.] US EPA, NHEERL, EPHD, CIB, Res Triangle Pk, NC 27709 USA. [Matheson, Joanna] US Consumer Prod Safety Commiss, Bethesda, MD 20814 USA. [Jacobs, Abigail] US FDA, CDER, Silver Spring, MD 20993 USA. [Lowit, Anna] US EPA, OCSPP, OPP, HED, Washington, DC 20460 USA. [Casey, Warren] NIEHS, NIH, DNTP, NICEATM, Res Triangle Pk, NC 27709 USA. RP Casey, W (reprint author), NIEHS, NIH, DNTP, NICEATM, POB 12233, Res Triangle Pk, NC 27709 USA. EM warren.casey@nih.gov OI Kleinstreuer, Nicole/0000-0002-7914-3682 FU federal funds from the NIEHS, NIH of NICEATM [HHSN273201500010C] FX The authors thank Drs D. Germolec, B.A. Merrick, R. Luebke and M. Ward for their thoughtful critical review of this manuscript. This project was funded in whole or in part with federal funds from the NIEHS, NIH under contract HHSN273201500010C to ILS in support of NICEATM. NR 77 TC 8 Z9 8 U1 4 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD SEP PY 2016 VL 36 IS 9 BP 1150 EP 1162 DI 10.1002/jat.3281 PG 13 WC Toxicology SC Toxicology GA DR5PD UT WOS:000379954000008 PM 26851134 ER PT J AU Deal, S Wambaugh, J Judson, R Mosher, S Radio, N Houck, K Padilla, S AF Deal, Samantha Wambaugh, John Judson, Richard Mosher, Shad Radio, Nick Houck, Keith Padilla, Stephanie TI Development of a quantitative morphological assessment of toxicant-treated zebrafish larvae using brightfield imaging and high-content analysis SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE zebrafish; screening; dysmorphology; high-content imaging; toxicity ID LOCOMOTOR-ACTIVITY; EMBRYOS; TOXICITY; EXPOSURE; CADMIUM; ASSAY; CHEMICALS; MODELS; SYSTEM AB One of the rate-limiting procedures in a developmental zebrafish screen is the morphological assessment of each larva. Most researchers opt for a time-consuming, structured visual assessment by trained human observer(s). The present studies were designed to develop a more objective, accurate and rapid method for screening zebrafish for dysmorphology. Instead of the very detailed human assessment, we have developed the computational malformation index, which combines the use of high-content imaging with a very brief human visual assessment. Each larva was quickly assessed by a human observer (basic visual assessment), killed, fixed and assessed for dysmorphology with the Zebratox V4 BioApplication using the Cellomics (R) ArrayScan (R) V-TI high-content image analysis platform. The basic visual assessment adds in-life parameters, and the high-content analysis assesses each individual larva for various features (total area, width, spine length, head-tail length, length-width ratio, perimeter-area ratio). In developing the computational malformation index, a training set of hundreds of embryos treated with hundreds of chemicals were visually assessed using the basic or detailed method. In the second phase, we assessed both the stability of these high-content measurements and its performance using a test set of zebrafish treated with a dose range of two reference chemicals (trans-retinoic acid or cadmium). We found the measures were stable for at least 1 week and comparison of these automated measures to detailed visual inspection of the larvae showed excellent congruence. Our computational malformation index provides an objective manner for rapid phenotypic brightfield assessment of individual larva in a developmental zebrafish assay. Copyright (c) 2016 John Wiley & Sons, Ltd. We developed a Computational Malformation Index that provides an objective manner for rapid phenotypic brightfield assessment of individual zebrafish larva in a developmental assay. By combining the use of high-content imaging with a very abbreviated human visual assessment, each larva can be quickly assessed for both detailed features as well as in-life parameters. Further, stability of these high-content measurements and the index performance were verified using a test set of zebrafish treated with two reference chemicals. C1 [Deal, Samantha; Wambaugh, John; Judson, Richard; Mosher, Shad; Houck, Keith] US EPA, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. [Radio, Nick] Thermo Fisher Sci Cellular Imaging & Anal, Pittsburgh, PA USA. [Padilla, Stephanie] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC USA. [Deal, Samantha] Baylor Coll Med, Dept Pediat, Div Pediat Neurol & Dev Neurosci, 6701 Fannin St, Houston, TX 77030 USA. RP Padilla, S (reprint author), US EPA, Integrated Syst Toxicol Branch B105 03, Res Triangle Pk, NC 27711 USA. EM Padilla.Stephanie@epa.gov OI Wambaugh, John/0000-0002-4024-534X NR 31 TC 0 Z9 0 U1 6 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD SEP PY 2016 VL 36 IS 9 BP 1214 EP 1222 DI 10.1002/jat.3290 PG 9 WC Toxicology SC Toxicology GA DR5PD UT WOS:000379954000014 PM 26924781 ER PT J AU Mikulcic, H Cabezas, H Vujanovic, M Duic, N AF Mikulcic, Hrvoje Cabezas, Heriberto Vujanovic, Milan Duic, Neven TI Environmental assessment of different cement manufacturing processes based on Emergy and Ecological Footprint analysis SO JOURNAL OF CLEANER PRODUCTION LA English DT Article DE Sustainable cement production; Cement industry; Mitigation scenario; Emergy; Ecological Footprint ID CO2 EMISSION REDUCTION; CARBON FOOTPRINT; ENERGY EFFICIENCY; PULVERIZED COAL; INDUSTRY; CHINA; CALCINER; BIOMASS; OPTIMIZATION; IMPROVEMENT AB Due to its high environmental impact and energy intensive production, the cement industry needs to adopt more energy efficient technologies to reduce its demand for fossil fuels and impact on the environment. Bearing in mind that cement is the most widely used material for housing and modern infrastructure, the aim of this paper is to analyse the Emergy and Ecological Footprint of different cement manufacturing processes for a particular cement plant. There are several mitigation measures that can be incorporated in the cement manufacturing process to reduce the demand for fossil fuels and consequently reduce the CO2 emissions. The mitigation measures considered in this paper were the use of alternative fuels and a more energy efficient kiln process. In order to estimate the sustainability effect of the aforementioned measures, Emergy and Ecological Footprint were calculated for four different scenarios. The results show that Emergy, due to the high input mass of raw material needed for clinker production, stays at about the same level. However, for the Ecological Footprint, the results show that by combining the use of alternative fuels together with a more energy efficient kiln process, the environmental impact of the cement manufacturing process can be lowered. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Mikulcic, Hrvoje; Vujanovic, Milan; Duic, Neven] Univ Zagreb, Fac Mech Engn & Naval Architecture, Ivana Lucica 5, Zagreb 10000, Croatia. [Cabezas, Heriberto] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. RP Mikulcic, H (reprint author), Univ Zagreb, Fac Mech Engn & Naval Architecture, Ivana Lucica 5, Zagreb 10000, Croatia. EM hrvoje.mikulcic@fsb.hr; Cabezas.Heriberto@epa.gov; milan.vujanovic@fsb.hr; neven.duic@fsb.hr RI Duic, Neven/J-5242-2012 OI Duic, Neven/0000-0003-2376-6466 NR 48 TC 9 Z9 9 U1 16 U2 40 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0959-6526 EI 1879-1786 J9 J CLEAN PROD JI J. Clean Prod. PD SEP 1 PY 2016 VL 130 BP 213 EP 221 DI 10.1016/j.jclepro.2016.01.087 PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA DQ7FB UT WOS:000379371200022 ER PT J AU Williams, J Pleil, J AF Williams, Jonathan Pleil, Joachim TI Crowd-based breath analysis: assessing behavior, activity, exposures, and emotional response of people in groups SO JOURNAL OF BREATH RESEARCH LA English DT Editorial Material DE crowd breath; mood and emotion; real-time MS; PTR-MS ID VOLATILE ORGANIC-COMPOUNDS; REACTION MASS-SPECTROMETRY; EXHALED BREATH; HUMAN EXPOSOME; BIOMARKERS; EMISSIONS; ISOPRENE; CHROMATOGRAPHY; COMMUNICATION; ATMOSPHERE AB A new concept for exhaled breath analysis has emerged wherein groups, or even crowds of people are simultaneously sampled in enclosed environments to detect overall trends in their activities and recent exposures. The basic idea is to correlate the temporal profile of known breath markers such as carbon dioxide, isoprene, or acetone with all other volatile organics in the air space. Those that trend similarly in time are designated as breath constituents. The ultimate goal of this work is to develop technology for assessing group based behaviors, chemical exposures or even changes in stress or mood. Applications are myriad ranging from chemical dose/toxicity screening to health and stress status for national security diagnostics. The basic technology employs real-time mass spectrometry capable of simultaneously measuring volatile chemicals and endogenous breath markers. C1 [Williams, Jonathan] Max Planck Inst Chem, Mainz, Germany. [Pleil, Joachim] US EPA, Res Triangle Pk, NC 27711 USA. RP Williams, J (reprint author), Max Planck Inst Chem, Mainz, Germany. EM Jonathan.Williams@mpic.de; pleil.joachim@epa.gov NR 54 TC 2 Z9 2 U1 13 U2 26 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1752-7155 EI 1752-7163 J9 J BREATH RES JI J. Breath Res. PD SEP PY 2016 VL 10 IS 3 AR 032001 DI 10.1088/1752-7155/10/3/032001 PG 5 WC Biochemical Research Methods; Respiratory System SC Biochemistry & Molecular Biology; Respiratory System GA DQ1ZJ UT WOS:000378999600001 PM 27341381 ER PT J AU Zhao, L Shaffer, F Robinson, B King, T D'Ambrose, C Pan, Z Gao, F Miller, RS Conmy, RN Boufadel, MC AF Zhao, Lin Shaffer, Franklin Robinson, Brian King, Thomas D'Ambrose, Christopher Pan, Zhong Gao, Feng Miller, Richard S. Conmy, Robyn N. Boufadel, Michel C. TI Underwater oil jet: Hydrodynamics and droplet size distribution SO CHEMICAL ENGINEERING JOURNAL LA English DT Article DE Subsurface oil release; Oil spill; Large scale experiment; Plume trajectory; Droplet size distribution; Ohmsett wave tank ID WATER-HORIZON OIL; GAS BLOWOUTS; BUOYANT JETS; SUBSEA OIL; CRUDE-OIL; FLOW-RATE; MODEL; BREAKUP; SIMULATION; ATOMIZATION AB We conducted a large scale experiment of underwater oil release of 6.3 L/s through a 25.4 mm (one inch) horizontal pipe. Detailed measurements of plume trajectory, velocity, oil droplet size distribution, and oil holdup were obtained. The obtained experimental data were used for the validation of the models JETLAG and VDROP-J. Key findings include: (1) formation of two plumes, one due to momentum and subsequently plume buoyancy, and another due mostly to the buoyancy of individual oil droplets that separate upward from the first plume; (2) modeling results indicated that the traditional miscible plume models matched the momentum and buoyancy plume, but were not able to simulate the upward motion plume induced by individual oil droplets; (3) high resolution images in the jet primary breakup region showed the formation of ligaments and drops in a process known as "primary breakup". These threads re-entered the plume to re-break in a process known as "secondary breakup"; (4) the plume velocity was highly heterogeneous with regions of high velocity surrounded by stagnant regions for various durations. The results from this study revealed that the primary breakup is a key factor for quantifying the droplet size distribution which plays a crucial role in determining the ultimate fate and transport of the released oil in the marine environment. The observed spatial heterogeneity in the oil plume implies that the effectiveness of applied dispersants may vary greatly when applying directly in the discharged oil flow. (C) 2016 Elsevier B.V. All rights reserved. C1 [Zhao, Lin; D'Ambrose, Christopher; Pan, Zhong; Gao, Feng; Boufadel, Michel C.] New Jersey Inst Technol, Dept Civil & Environm Engn, Ctr Nat Resources Dev & Protect, Newark, NJ 07102 USA. [Shaffer, Franklin] Natl Energy Technol Lab, Pittsburgh, PA 15236 USA. [Robinson, Brian; King, Thomas] Bedford Inst Oceanog, Dept Fisheries & Oceans, Dartmouth, NS, Canada. [Miller, Richard S.] Clemson Univ, Dept Mech Engn, Clemson, SC 29634 USA. [Conmy, Robyn N.] US EPA, Natl Risk Management Res Lab, Off Res & Dev, Cincinnati, OH 45268 USA. RP Boufadel, MC (reprint author), New Jersey Inst Technol, Dept Civil & Environm Engn, Ctr Nat Resources Dev & Protect, Newark, NJ 07102 USA. EM boufadel@gmail.com FU Bureau of Safety and Environmental Enforcement [1027]; Department of Fisheries and Ocean Canada (DFO) [F5211-130060]; Gulf of Mexico Research Initiative through the Consortium DROPPS II FX This research was made possible through funding from the Bureau of Safety and Environmental Enforcement, Project # 1027 (2014); the Department of Fisheries and Ocean Canada (DFO), Contract No. F5211-130060; and the Gulf of Mexico Research Initiative through the Consortium DROPPS II. Data are publicly available through the Gulf of Mexico Research Initiative Information & Data Cooperative (GRIIDC) at https://data.gulfresearchinitiative.org (doi:10.7266/N7D798DN). However, no endorsement of these sponsors is implied. NR 35 TC 1 Z9 1 U1 14 U2 58 PU ELSEVIER SCIENCE SA PI LAUSANNE PA PO BOX 564, 1001 LAUSANNE, SWITZERLAND SN 1385-8947 EI 1873-3212 J9 CHEM ENG J JI Chem. Eng. J. PD SEP 1 PY 2016 VL 299 BP 292 EP 303 DI 10.1016/j.cej.2016.04.061 PG 12 WC Engineering, Environmental; Engineering, Chemical SC Engineering GA DO5ON UT WOS:000377832500035 ER PT J AU Pennino, MJ Kaushal, SS Mayer, PM Utz, RM Cooper, CA AF Pennino, Michael J. Kaushal, Sujay S. Mayer, Paul M. Utz, Ryan M. Cooper, Curtis A. TI Stream restoration and sewers impact sources and fluxes of water, carbon, and nutrients in urban watersheds SO HYDROLOGY AND EARTH SYSTEM SCIENCES LA English DT Article ID DISSOLVED ORGANIC-MATTER; SURFACE-WATER; LAND-USE; WASTE-WATER; FLUORESCENCE SPECTROSCOPY; ARTIFICIAL SWEETENERS; FORESTED WATERSHEDS; IMPERVIOUS SURFACES; HYDROLOGIC RESPONSE; TRANSIENT STORAGE AB An improved understanding of sources and timing of water, carbon, and nutrient fluxes associated with urban infrastructure and stream restoration is critical for guiding effective watershed management globally. We investigated how sources, fluxes, and flowpaths of water, carbon (C), nitrogen (N), and phosphorus (P) shift in response to differences in urban stream restoration and sewer infrastructure. We compared an urban restored stream with two urban degraded streams draining varying levels of urban development and one stream with upland stormwater management systems over a 3-year period. We found that there was significantly decreased peak discharge in response to precipitation events following stream restoration. Similarly, we found that the restored stream showed significantly lower (p < 0.05) monthly peak runoff (9.4 +/- 1.0mm day(-1)) compared with two urban degraded streams (ranging from 44.9 +/- 4.5 to 55.4 +/- 5.8mm day(-1)) draining higher impervious surface cover, and the stream-draining stormwater management systems and less impervious surface cover in its watershed (13.2 +/- 1.9 mm day(-1)). The restored stream exported most carbon, nitrogen, and phosphorus at relatively lower streamflow than the two more urban catchments, which exported most carbon and nutrients at higher streamflow. Annual exports of total carbon (6.6 +/- 0.5 kg ha(-1) yr(-1)), total nitrogen (4.5 +/- 0.3 kg ha(-1) yr (-1)), and total phosphorus (161 +/- 15 kg ha(-1) yr(-1)) were significantly lower in the restored stream compared to both urban degraded streams (p < 0.05), but statistically similar to the stream draining stormwater management systems, for N exports. However, nitrate isotope data suggested that 55 +/- 1% of the nitrate in the urban restored stream was derived from leaky sanitary sewers (during baseflow), statistically similar to the urban degraded streams. These isotopic results as well as additional tracers, including fluoride (added to drinking water) and iodide (contained in dietary salt), suggested that groundwater contamination was a major source of urban nutrient fluxes, which has been less considered compared to upland sources. Overall, leaking sewer pipes are a problem globally and our results suggest that combining stream restoration with restoration of aging sewer pipes can be critical to more effectively minimizing urban nonpoint nutrient sources. The sources, fluxes, and flowpaths of groundwater should be prioritized in management efforts to improve stream restoration by locating hydrologic hot spots where stream restoration is most likely to succeed. C1 [Pennino, Michael J.; Mayer, Paul M.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Western Ecol Div, Corvallis, OR 97333 USA. [Kaushal, Sujay S.] Univ Maryland, Dept Geol & Earth Syst Sci, Interdisciplinary Ctr, College Pk, MD 20742 USA. [Utz, Ryan M.] Chatham Univ, Falk Sch Sustainabil, Pittsburgh, PA USA. [Cooper, Curtis A.] Washington Dept Ecol, Environm Assessment Program, Olympia, WA USA. RP Pennino, MJ (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Western Ecol Div, Corvallis, OR 97333 USA. EM michael.pennino@gmail.com FU EPA NNEMS Award [2010-309]; NSF Graduate Research Fellowship Program [DGE-1144243]; NSF [DGE 0549469, EF-0709659, DBI 0640300, CBET 1058502, CBET 1058038, EAR 1521224]; NASA [NASA NNX11AM28G]; Maryland Sea [SA7528085-U, R/WS-2, NA05OAR4171042]; Baltimore Ecosystem Study LTER project [NSF DEB-1027188]; US Environmental Protection Agency through its Office of Research and Development FX This research was supported by EPA NNEMS Award 2010-309; the NSF Graduate Research Fellowship Program under grant no. DGE-1144243; NSF Awards DGE 0549469, EF-0709659, DBI 0640300, CBET 1058502, CBET 1058038, and EAR 1521224; NASA grant NASA NNX11AM28G; Maryland Sea Grant Awards SA7528085-U, R/WS-2, and NA05OAR4171042; and Baltimore Ecosystem Study LTER project (NSF DEB-1027188). We thank Tiana Pennino for field assistance, Garth Lindner for providing rating curves for two of the stream gauges, Stu Schwartz for extensive guidance on statistical analysis for nutrient export calculations, and Steve Stewart and Robert Hirsch from the Baltimore County Department of Environmental Protection and Sustainability for providing stormwater management data. Claire Welty and Andrew Miller provided help interpreting water isotope and streamflow data, respectively, and helpful suggestions and discussions on an earlier version of the manuscript. The US Environmental Protection Agency, through its Office of Research and Development, funded and managed, or partially funded and collaborated in, the research described herein. It has been subjected to the agency's peer and administrative review and has been approved for external publication. Any opinions expressed in this paper are those of the author(s) and do not necessarily reflect the views of the agency, therefore, no official endorsement should be inferred. Any mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 138 TC 0 Z9 0 U1 34 U2 34 PU COPERNICUS GESELLSCHAFT MBH PI GOTTINGEN PA BAHNHOFSALLEE 1E, GOTTINGEN, 37081, GERMANY SN 1027-5606 EI 1607-7938 J9 HYDROL EARTH SYST SC JI Hydrol. Earth Syst. Sci. PD AUG 26 PY 2016 VL 20 IS 8 BP 3419 EP 3439 DI 10.5194/hess-20-3419-2016 PG 21 WC Geosciences, Multidisciplinary; Water Resources SC Geology; Water Resources GA DW7QQ UT WOS:000383846500001 ER PT J AU Baranizadeh, E Murphy, BN Julin, J Falahat, S Reddington, CL Arola, A Ahlm, L Mikkonen, S Fountoukis, C Patoulias, D Minikin, A Hamburger, T Laaksonen, A Pandis, SN Vehkamaki, H Lehtinen, KEJ Riipinen, I AF Baranizadeh, Elham Murphy, Benjamin N. Julin, Jan Falahat, Saeed Reddington, Carly L. Arola, Antti Ahlm, Lars Mikkonen, Santtu Fountoukis, Christos Patoulias, David Minikin, Andreas Hamburger, Thomas Laaksonen, Ari Pandis, Spyros N. Vehkamaki, Hanna Lehtinen, Kari E. J. Riipinen, Ilona TI Implementation of state-of-the-art ternary new-particle formation scheme to the regional chemical transport model PMCAMx-UF in Europe SO GEOSCIENTIFIC MODEL DEVELOPMENT LA English DT Article ID QUALITY INTERACTIONS EUCAARI; INTEGRATING AEROSOL RESEARCH; BOUNDARY-LAYER NUCLEATION; WILD-LAND FIRES; SULFURIC-ACID; AIR-QUALITY; NUMBER CONCENTRATIONS; SIZE DISTRIBUTION; GLOBAL SCALES; ATMOSPHERIC PARTICLES AB The particle formation scheme within PMCAMx-UF, a three-dimensional chemical transport model, was updated with particle formation rates for the ternary H2SO4-NH3-H2O pathway simulated by the Atmospheric Cluster Dynamics Code (ACDC) using quantum chemical input data. The model was applied over Europe for May 2008, during which the EUCAARI-LONGREX (European Aerosol Cloud Climate and Air Quality Interactions-Long-Range Experiment) campaign was carried out, providing aircraft vertical profiles of aerosol number concentrations. The updated model reproduces the observed number concentrations of particles larger than 4 nm within 1 order of magnitude throughout the atmospheric column. This agreement is encouraging considering the fact that no semi-empirical fitting was needed to obtain realistic particle formation rates. The cloud adjustment scheme for modifying the photolysis rate profiles within PMCAMx-UF was also updated with the TUV (Tropospheric Ultraviolet and Visible) radiative-transfer model. Results show that, although the effect of the new cloud adjustment scheme on total number concentrations is small, enhanced new-particle formation is predicted near cloudy regions. This is due to the enhanced radiation above and in the vicinity of the clouds, which in turn leads to higher production of sulfuric acid. The sensitivity of the results to including emissions from natural sources is also discussed. C1 [Baranizadeh, Elham; Julin, Jan; Mikkonen, Santtu; Laaksonen, Ari; Lehtinen, Kari E. J.] Univ Eastern Finland, Dept Appl Phys, Aerosol Phys Grp, Kuopio, Finland. [Murphy, Benjamin N.; Julin, Jan; Falahat, Saeed; Ahlm, Lars; Riipinen, Ilona] Stockholm Univ, Dept Environm Sci & Analyt Chem ACES, Stockholm, Sweden. [Reddington, Carly L.] Univ Leeds, Sch Earth & Environm, Inst Climate & Atmospher Sci, Leeds, W Yorkshire, England. [Arola, Antti; Lehtinen, Kari E. J.] Finnish Meteorol Inst, Atmospher Res Ctr Eastern Finland, Kuopio, Finland. [Fountoukis, Christos] Hamad Bin Khalifa Univ, Qatar Fdn, QEERI, Doha, Qatar. [Patoulias, David; Pandis, Spyros N.] Univ Patras, Dept Chem Engn, Patras, Greece. [Minikin, Andreas] Deutsch Zentrum Luft & Raumfahrt DLR, Inst Phys Atmosphare, Oberpfaffenhofen, Germany. [Hamburger, Thomas] Norwegian Inst Air Res NILU, Atmosphere & Climate Dept ATMOS, Oslo, Norway. [Laaksonen, Ari] Finnish Meteorol Inst, Climate Res Unit, Helsinki, Finland. [Pandis, Spyros N.] Fdn Res & Technol Hellas ICEHT FORTH, Inst Chem Engn Sci, Patras, Greece. [Pandis, Spyros N.] Carnegie Mellon Univ, Dept Chem Engn, Pittsburgh, PA 15213 USA. [Vehkamaki, Hanna] Univ Helsinki, Dept Phys, Div Atmospher Sci, Helsinki, Finland. [Murphy, Benjamin N.] US EPA, Natl Exposure Res Lab, Durham, NC USA. [Falahat, Saeed] SMHI, Norrkoping, Sweden. [Minikin, Andreas] Deutsch Zentrum Luft & Raumfahrt DLR, Flugexpt, Oberpfaffenhofen, Germany. [Hamburger, Thomas] Fed Off Radiat Protect BfS, Neuherberg, Germany. RP Riipinen, I (reprint author), Stockholm Univ, Dept Environm Sci & Analyt Chem ACES, Stockholm, Sweden. EM ilona.riipinen@aces.su.se RI Mikkonen, Santtu/E-8568-2011; Vehkamaki, Hanna/A-8262-2008; OI Mikkonen, Santtu/0000-0003-0595-0657; Vehkamaki, Hanna/0000-0002-5018-1255; Julin, Jan/0000-0002-4845-1631; Arola, Antti/0000-0002-9220-0194 FU EUCAARI project [036833-2]; DLR; UK Natural Environment Research Council through the APPRAISE programme [NE/E01108X/1]; Academy of Finland Centre of Excellence Programme [272041] FX We gratefully acknowledge Oona Kupiainen-Maatta for providing the ACDC-simulation data and generating the lookup table. Tinja Olenius is acknowledged for discussions and technical support related to ACDC, Radovan Krejci for providing the EUCAARI-LONGREX data, and Samuel Lowe for comments on the language. Falcon measurements and data analysis were funded by EUCAARI project no. 036833-2 and by DLR. The UK aircraft experiment was supported through EUCAARI and the UK Natural Environment Research Council through the APPRAISE programme, grant NE/E01108X/1. The authors also thank the Academy of Finland Centre of Excellence Programme (project number 272041), the Nordic Centre of Excellence CRAICC, Academy of Finland, ERC-StG-ATMOGAIN (278277) and ERC-StG_MOCAPAF (257360). NR 72 TC 1 Z9 1 U1 20 U2 20 PU COPERNICUS GESELLSCHAFT MBH PI GOTTINGEN PA BAHNHOFSALLEE 1E, GOTTINGEN, 37081, GERMANY SN 1991-959X EI 1991-9603 J9 GEOSCI MODEL DEV JI Geosci. Model Dev. PD AUG 22 PY 2016 VL 9 IS 8 BP 2741 EP 2754 DI 10.5194/gmd-9-2741-2016 PG 14 WC Geosciences, Multidisciplinary SC Geology GA DW6XJ UT WOS:000383794400001 ER PT J AU Stone, H See, D Smiley, A Ellingson, A Schimmoeller, J Oudejans, L AF Stone, Harry See, David Smiley, Autumn Ellingson, Anthony Schimmoeller, Jessica Oudejans, Lukas TI Surface decontamination for blister agents Lewisite, sulfur mustard and agent yellow, a Lewisite and sulfur mustard mixture SO JOURNAL OF HAZARDOUS MATERIALS LA English DT Article DE Chemical warfare agent; Blister agent; Decontamination; Sulfur mustard; Lewisite ID CHEMICAL WARFARE AGENTS AB Sulfur mustard (HD) and Lewisite (L) are blister agents that have a high potential for terrorist use; Agent Yellow (HL) is the eutectic mixture of HD and L Bench-scale testing was used to determine the residual amount of these chemical warfare agents remaining on three building materials (wood, metal and glass) after application of various decontaminants (household bleach, full strength and dilute; hydrogen peroxide 3% soliition; and EasyDECON (R) DF200). All decontaminants reduced the amount of L recovered from coupons. Application of dilute bleach showed little or no difference compared to natural attenuation in the amount of HD recovered from coupons. Full-strength bleach was the most effective of four decontaminants at reducing the amount of HD from coupons. Hydrogen peroxide (3% solution) and DF200 did decrease the amount of HD recovered from coupons more than natural attenuation (except DF200 against HD on metal), but substantial amounts of HD remained on some materials. Toxic HD by-products were generated by hydrogen peroxide treatment. The effectiveness of decontaminants was found to depend on agent, material, and decontaminant. Increased decontaminant reaction time (60 min rather than 30 min) did not significantly increase effectiveness. Published by Elsevier B.V. C1 [Stone, Harry] Battelle Mem Inst, 10300 Alliance Rd Suite 155, Cincinnati, OH 45242 USA. [See, David; Smiley, Autumn; Ellingson, Anthony; Schimmoeller, Jessica] Battelle Mem Inst, 1425 Plain City Georgesville, West Jefferson, OH 43162 USA. [Oudejans, Lukas] US EPA, Natl Homeland Secur Res Ctr, 109 TW Alexander Dr,Mail Code E343-06, Res Triangle Pk, NC 27709 USA. RP Oudejans, L (reprint author), US EPA, Natl Homeland Secur Res Ctr, 109 TW Alexander Dr,Mail Code E343-06, Res Triangle Pk, NC 27709 USA. EM oudejans.lukas@epa.gov FU U.S. Environmental Protection Agency through its Office of Research and Development [EP-C-10-001, EP-C-11-038] FX The U.S. Environmental Protection Agency through its Office of Research and Development funded and managed the research described here under Contract No. EP-C-10-001 and EP-C-11-038 to Battelle. It has been subjected to the Agency's review and has been approved for publication. Note that approval does not signify that the contents necessarily reflect the views of the Agency. Mention of trade names, products, or services does not convey official EPA approval, endorsement, or recommendation. NR 18 TC 1 Z9 1 U1 12 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3894 EI 1873-3336 J9 J HAZARD MATER JI J. Hazard. Mater. PD AUG 15 PY 2016 VL 314 BP 59 EP 66 DI 10.1016/j.jhazmat.2016.04.020 PG 8 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DO4FX UT WOS:000377738300007 PM 27107236 ER PT J AU McEwen, AR Hsu-Kim, H Robins, NA Hagan, NA Halabi, S Barras, O Richter, DD Vandenberg, JJ AF McEwen, Abigail R. Hsu-Kim, Heileen Robins, Nicholas A. Hagan, Nicole A. Halabi, Susan Barras, Olivo Richter, Daniel deB. Vandenberg, John J. TI Residential metal contamination and potential health risks of exposure in adobe brick houses in Potosi, Bolivia SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Bolivia; Adobe; Bioaccessibility; Mercury; Arsenic; Lead ID BIOACCESSIBILITY; POLLUTION; BIOAVAILABILITY; ALTIPLANO; CHILDREN; MERCURY; WASTE; SOILS; LEAD AB Potosi, Bolivia, is the site of centuries of historic and present-daymining of the Cerro Rico, a mountain known for its rich polymetallic deposits, and was the site of large-scale Colonial era silver refining operations. In this study, the concentrations of several metal and metalloid elements were quantified in adobe brick, dirt floor, and surface dust samples from 49 houses in Potosi. Median concentrations of total mercury (Hg), lead (Pb), and arsenic (As) were significantly greater than concentrations measured in Sucre, Bolivia, a non-mining town, and exceeded US-based soil screening levels. Adobe brick samples were further analyzed for bioaccessible concentrations of trace elements using a simulated gastric fluid (GF) extraction. Median GF extractable concentrations of Hg, As, and Pb were 0.085, 13.9, and 32.2% of the total element concentration, respectively. Total and GF extractable concentrations of Hg, As, and Pb were used to estimate exposure and potential health risks to children following incidental ingestion of adobe brick particles. Risks were assessed using a range of potential ingestion rates (50-1000 mg/day). Overall, the results of the risk assessment show that the majority of households sampled contained concentrations of bioaccessible Pb and As, but not Hg, that represent a potential health risk. Even at the lowest ingestion rate considered, the majority of households exceeded the risk threshold for Pb, indicating that the concentrations of this metal are of particular concern. To our knowledge, this is the first study to quantify key trace elements in building materials in adobe brick houses and the results indicate that these houses are a potential source of exposure to metals and metalloids in South American mining communities. Additional studies are needed to fully characterize personal exposure and to understand potential adverse health outcomes within the community. (C) 2016 Elsevier B.V. All rights reserved. C1 [McEwen, Abigail R.; Richter, Daniel deB.] Duke Univ, Nicholas Sch Environm, LSRC, Box 90328, Durham, NC 27708 USA. [Hsu-Kim, Heileen] Duke Univ, Dept Civil & Environm Engn, Box 90287, Durham, NC 27708 USA. [Robins, Nicholas A.] N Carolina State Univ, Dept Hist, 467 Withers Hall, Raleigh, NC 27695 USA. [Hagan, Nicole A.] US EPA, Off Air Qual Planning & Stand, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Halabi, Susan] Duke Univ, Dept Biostat & Bioinformat, Med Ctr, Box 2717, Durham, NC 27710 USA. [Barras, Olivo] Environm Hlth Council, Potosi, Bolivia. [Vandenberg, John J.] US EPA, Off Res & Dev, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. RP McEwen, AR (reprint author), Duke Univ, Nicholas Sch Environm, LSRC, Box 90328, Durham, NC 27708 USA. EM mcewen.abigail@gmail.com; hsukim@duke.edu; narobins@ncsu.edu; hagan.nicole@epa.gov; susan.halabi@duke.edu; mdmbolivia@yahoo.fr; drichter@duke.edu; vandenberg.john@epa.gov RI Hsu-Kim, Heileen/A-5409-2008; OI Hsu-Kim, Heileen/0000-0003-0675-4308; Vandenberg, John/0000-0003-2619-9460 FU Duke Global Health Institute; Center for Environmental Implications of Nanotechnology [NSF EF-08-30093]; National Science Foundation FX This work was supported in part by the Duke Global Health Institute and the Center for Environmental Implications of Nanotechnology (NSF EF-08-30093). The authors would like to thank Kaitlyn Porter, for laboratory analytical assistance, and Jacqueline Moya, for thoughtful guidance on ingestion rates for children. The authors would like to thank collaborators from Musaq Nan for their tireless efforts and assistance in the field in Potosi and Paul Heine for coordinating the soil importation and containments as a USDA permitted Soil Containment Officer. Daniel Richter thanks the National Science Foundation for financial support through the Geosciences Directorate's Critical Zone Observatory program. Special thanks to the residents of Potosi who participated in this study. NR 38 TC 0 Z9 0 U1 8 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 15 PY 2016 VL 562 BP 237 EP 246 DI 10.1016/j.scitotenv.2016.03.152 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DN9AW UT WOS:000377372400024 PM 27100004 ER PT J AU Daughton, CG AF Daughton, Christian G. TI Pharmaceuticals and the Environment (PiE): Evolution and impact of the published literature revealed by bibliometric analysis SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Review DE Pharmaceuticals; Environmental contaminants; History; Bibliometrics; Most cited ID MULTIXENOBIOTIC RESISTANCE MECHANISM; SEWAGE-TREATMENT PLANTS; PERSONAL CARE PRODUCTS; CHROMATOGRAPHY-MASS SPECTROMETRY; SOLID-PHASE EXTRACTION; ENDOCRINE-DISRUPTING CHEMICALS; PROMOTING HUMAN HEALTH; WATER-TREATMENT PLANTS; TO-CRADLE STEWARDSHIP; TREATED WASTE-WATER AB The evolution and impact of the published literature surrounding the transdisciplinary, multifaceted topic of pharmaceuticals as contaminants in the environment is examined for the first time in an historical context. The preponderance of literature cited in this examination represents the earlier works. As an historical chronology, the focus is on the emergence of key, specific aspects of the overall topic (often termed PiE) in the published literature and on the most highly cited works. This examination is not a conventional, technical review of the literature; as such, little attention was devoted to the more recent literature. The many dimensions involved with PiE span over 70 years of published literature. Some articles began to appear in published works in the 1940s and earlier, while others only began to receive attention in the 1990s and later. Decades of early research on what at the time seemed to be disconnected topics eventually coalesced in the mid-to-late 1990s around a number of interconnected concerns and issues that now comprise PiE. Major objectives are to provide a new perspective to the topic, to facilitate more efficient and effective review of the literature by others, and to recognize the more significant, seminal contributions to the advancement of PiE as a field of research. Some of the most highly cited articles in all of environmental science now involve PiE. As of April 2015, a core group of 385 PiE articles had each received at least 200 citations; one had received 5424 citations. But hundreds of additional articles also played important roles in the evolution and advancement of the field. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Daughton, Christian G.] US EPA, Environm Futures Anal Branch, Syst Exposure Div, Natl Exposure Res Lab,Off Res & Dev, 944 East Harmon Ave, Las Vegas, NV 89119 USA. RP Daughton, CG (reprint author), US EPA, Environm Futures Anal Branch, Syst Exposure Div, Natl Exposure Res Lab,Off Res & Dev, 944 East Harmon Ave, Las Vegas, NV 89119 USA. EM daughton.christian@epa.gov FU United States Environmental Protection Agency FX The information in this document has been funded wholly by the United States Environmental Protection Agency under. It has been subjected to the Agency's peer and administrative review and has been approved for publication as an EPA document. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. Greatly appreciated were the assistance of MST Scuderi (SEEP, US EPA, Las Vegas) in the maintenance of the EndNote bibliographic database and the technical reviews of this manuscript by Dr. Susan T. Glassmeyer (NERL, ORD, US EPA, Cincinnati, OH) and MST Scuderi. NR 430 TC 6 Z9 6 U1 40 U2 99 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 15 PY 2016 VL 562 BP 391 EP 426 DI 10.1016/j.scitotenv.2016.03.109 PG 36 WC Environmental Sciences SC Environmental Sciences & Ecology GA DN9AW UT WOS:000377372400039 PM 27104492 ER PT J AU Vesper, S Wymer, L Cox, D Dewalt, G AF Vesper, Stephen Wymer, Larry Cox, David Dewalt, Gary TI Populations of some molds in water-damaged homes may differ if the home was constructed with gypsum drywall compared to plaster SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Mold; Plaster; Gypsum drywall; ERMI ID RELATIVE MOLDINESS INDEX; ASTHMATIC-CHILDREN; VALUES; ASPERGILLUS AB Starting in the 1940s, gypsum drywall began replacing plaster and lathe in the U.S. home construction industry. Our goal was to evaluate whether some mold populations differ in water-damaged homes primarily constructed with gypsum drywall compared to plaster. The dust samples from the 2006 Department of Housing and Urban Development's (HUD) American Health Homes Survey (AHHS) were the subject of this analysis. The concentrations of the 36 Environmental Relative Moldiness Index (ERMI) molds were compared in homes of different ages. The homes (n = 301) were built between 1878 and 2005. Homes with ERMI values >5 (n = 126) were defined as water-damaged. Homes with ERMI values >5 were divided in the years 1976 to 1977 into two groups, i.e., older (n = 61) and newer (n = 65). Newer water-damaged homes had significantly (p = 0.002) higher mean ERMI values than older water-damaged homes, 11.18 and 8.86, respectively. The Group 1 molds Aspergillus flavus, Ammophilus fumigatus, Aspergillus ochraceus, Cladosporium sphaerospermum and Trichoderma viride were found in significantly higher concentrations in newer compared to older high-ERMI homes. Some mold populations in water-damaged homes may have changed after the introduction of gypsum drywall. Published by Elsevier B.V. C1 [Vesper, Stephen; Wymer, Larry] US EPA, NERL, Cincinnati, OH 45268 USA. [Cox, David; Dewalt, Gary] QuanTech Inc, Arlington, VA USA. RP Vesper, S (reprint author), US EPA, NERL, Cincinnati, OH 45268 USA. EM vesper.stephen@epa.gov; Wymer.Larry@epa.gov; dcox@quantech.com; fgdewalt@comcast.net NR 17 TC 0 Z9 0 U1 4 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 15 PY 2016 VL 562 BP 446 EP 450 DI 10.1016/j.scitotenv.2016.04.067 PG 5 WC Environmental Sciences SC Environmental Sciences & Ecology GA DN9AW UT WOS:000377372400042 PM 27104493 ER PT J AU Hildenbrand, ZL Carlton, DD Fontenot, BE Meik, JM Walton, JL Thacker, JB Korlie, S Shelor, CP Kadjo, AF Clark, A Usenko, S Hamilton, JS Mach, PM Verbeck, GF Hudak, P Schug, KA AF Hildenbrand, Zacariah L. Carlton, Doug D., Jr. Fontenot, Brian E. Meik, Jesse M. Walton, Jayme L. Thacker, Jonathan B. Korlie, Stephanie Shelor, C. Phillip Kadjo, Akinde F. Clark, Adelaide Usenko, Sascha Hamilton, Jason S. Mach, Phillip M. Verbeck, Guido F. Hudak, Paul Schug, Kevin A. TI Temporal variation in groundwater quality in the Permian Basin of Texas, a region of increasing unconventional oil and gas development SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Unconventional oil and gas development; Groundwater; Permian Basin; Shale energy ID DRINKING-WATER WELLS; MARCELLUS; BROMIDE; CONTAMINATION; PENNSYLVANIA; CHLORIDE/BROMIDE; EXTRACTION; AQUIFERS; METHANE; RATIOS AB The recent expansion of natural gas and oil extraction using unconventional oil and gas development (UD) practices such as horizontal drilling and hydraulic fracturing has raised questions about the potential for environmental impacts. Prior research has focused on evaluations of air and water quality in particular regions without explicitly considering temporal variation; thus, little is known about the potential effects of UD activity on the environment over longer periods of time. Here, we present an assessment of private well water quality in an area of increasing UD activity over a period of 13 months. We analyzed samples from 42 private water wells located in three contiguous counties on the Eastern Shelf of the Permian Basin in Texas. This area has experienced a rise in UD activity in the last few years, and we analyzed samples in four separate time points to assess variation in groundwater quality over time as UD activities increased. We monitored general water quality parameters as well as several compounds used in UD activities. We found that some constituents remained stable over time, but others experienced significant variation over the period of study. Notable findings include significant changes in total organic carbon and pH along with ephemeral detections of ethanol, bromide, and dichloromethane after the initial sampling phase. These data provide insight into the potentially transient nature of compounds associated with groundwater contamination in areas experiencing UD activity. (C) 2016 Elsevier B. V. All rights reserved. C1 [Hildenbrand, Zacariah L.; Carlton, Doug D., Jr.; Fontenot, Brian E.; Meik, Jesse M.; Walton, Jayme L.; Schug, Kevin A.] Univ Texas Arlington, Collaborat Labs Environm Anal & Remediat, Arlington, TX 76019 USA. [Hildenbrand, Zacariah L.] Inform Environm LLC, 6060 N Cent Expressway,Suite 500, Dallas, TX 75206 USA. [Carlton, Doug D., Jr.; Thacker, Jonathan B.; Korlie, Stephanie; Shelor, C. Phillip; Kadjo, Akinde F.; Schug, Kevin A.] Univ Texas Arlington, Dept Chem & Biochem, 700 Planetarium Pl,Box 19065, Arlington, TX 76019 USA. [Meik, Jesse M.] Tarleton State Univ, Dept Biol Sci, Stephenville, TX 76401 USA. [Clark, Adelaide; Usenko, Sascha] Baylor Univ, Dept Environm Sci, Waco, TX 76706 USA. [Hamilton, Jason S.; Mach, Phillip M.; Verbeck, Guido F.] Univ N Texas, Dept Chem, Denton, TX 76203 USA. [Hudak, Paul] Univ N Texas, Dept Geog, Denton, TX 76203 USA. [Fontenot, Brian E.] US EPA, Water Qual Protect Div, Dallas, TX 75202 USA. RP Hildenbrand, ZL (reprint author), Inform Environm LLC, 6060 N Cent Expressway,Suite 500, Dallas, TX 75206 USA.; Schug, KA (reprint author), Univ Texas Arlington, Dept Chem & Biochem, 700 Planetarium Pl,Box 19065, Arlington, TX 76019 USA. EM zac@informenv.com; kschug@uta.edu RI Guenat, Heather/H-6528-2014 FU Shimadzu Institute for Research Technologies at the University of Texas at Arlington FX The authors thank financial support from the Shimadzu Institute for Research Technologies at the University of Texas at Arlington. We would like to thank J.P. Nicot and P. Dasgupta for their review of an earlier version of this manuscript. Most importantly, we would like to thank the well owners who participated in this study. NR 39 TC 7 Z9 7 U1 30 U2 59 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 15 PY 2016 VL 562 BP 906 EP 913 DI 10.1016/j.scitotenv.2016.04.144 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DN9AW UT WOS:000377372400088 PM 27125684 ER PT J AU King, DN Donohue, MJ Vesper, SJ Villegas, EN Ware, MW Vogel, ME Furlong, EF Kolpin, DW Glassmeyer, ST Pfaller, S AF King, Dawn N. Donohue, Maura J. Vesper, Stephen J. Villegas, Eric N. Ware, Michael W. Vogel, Megan E. Furlong, Edward F. Kolpin, Dana W. Glassmeyer, Susan T. Pfaller, Stacy TI Microbial pathogens in source and treated waters from drinking water treatment plants in the United States and implications for human health SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Pathogens; Drinking water; Treatment; Source water; Occurrence ID MYCOBACTERIUM-AVIUM; QUANTITATIVE PCR; LEGIONNAIRES-DISEASE; CRYPTOSPORIDIUM; LEGIONELLA; MONOCHLORAMINE; COLONIZATION; ASPERGILLUS; BIOFILMS; PARATUBERCULOSIS AB An occurrence survey was conducted on selected pathogens in source and treated drinking water collected from 25 drinking water treatment plants (DWTPs) in the United States. Water samples were analyzed for the protozoa Giardia and Cryptosporidium(EPA Method 1623); the fungi Aspergillus fumigatus, Aspergillus niger and Aspergillus terreus (quantitative PCR [qPCR]); and the bacteria Legionella pneumophila (qPCR), Mycobacterium avium, M. avium subspecies paratuberculosis, and Mycobacterium intracellulare (qPCR and culture). Cryptosporidium and Giardia were detected in 25% and in 46% of the source water samples, respectively (treated waters were not tested). Aspergillus fumigatus was the most commonly detected fungus in source waters (48%) but none of the three fungi were detected in treated water. Legionella pneumophila was detected in 25% of the source water samples but in only 4% of treated water samples. M. avium and M. intracellulare were both detected in 25% of source water, while all three mycobacteria were detected in 36% of treated water samples. Five species of mycobacteria, Mycobacterium mucogenicum, Mycobacterium phocaicum, Mycobacterium triplex, Mycobacterium fortuitum, and Mycobacterium lentiflavum were cultured from treated water samples. Although these DWTPs represent a fraction of those in the U.S., the results suggest that many of these pathogens are widespread in source waters but that treatment is generally effective in reducing them to below detection limits. The one exception is the mycobacteria, which were commonly detected in treated water, even when not detected in source waters. Published by Elsevier B.V. C1 [King, Dawn N.; Donohue, Maura J.; Vesper, Stephen J.; Villegas, Eric N.; Ware, Michael W.; Glassmeyer, Susan T.; Pfaller, Stacy] US EPA, Off Res & Dev, Natl Exposure Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. [Vogel, Megan E.] Univ Cincinnati, Coll Med, Dept Internal Med, 231 Albert Sabin Way, Cincinnati, OH 45229 USA. [Furlong, Edward F.] US Geol Survey, Denver Fed Ctr, POB 25585, Denver, CO 80225 USA. [Kolpin, Dana W.] US Geol Survey, 400 S Clinton St, Iowa City, IA 52240 USA. RP Pfaller, S (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. EM pfaller.stacy@epa.gov FU U.S. Environmental Protection Agency; U.S. Environmental Protection Agency [DW14922330]; USGS Toxic Substances Hydrology Program; USEPA's Office of Research and Development, Office of Water, Office of Chemical Safety and Pollution Prevention FX The authors declare no competing financial interest. The information in this document has been funded partially or wholly by the U.S. Environmental Protection Agency. The research described in this article has been funded in part by the U.S. Environmental Protection Agency through Interagency Agreement DW14922330 to the U.S. Geological Survey, and through programmatic support of the USGS Toxic Substances Hydrology Program and the USEPA's Office of Research and Development, Office of Water, Office of Chemical Safety and Pollution Prevention, and Region 8. Information Collection Rule approval for the Phase II Questionnaire was granted under USEPA ICR No. 2346.01, OMB Control No. 2080-0078. This manuscript has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the USEPA and mention of trade names or commercial products does not constitute endorsement or recommendation for use by USEPA. This document has been reviewed in accordance with USGS policy and approved for publication. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. The authors would like to thank all participating DWTPs for their involvement in the project and for their assistance in collecting the samples. NR 42 TC 1 Z9 1 U1 21 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 15 PY 2016 VL 562 BP 987 EP 995 DI 10.1016/j.scitotenv.2016.03.214 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DN9AW UT WOS:000377372400096 PM 27260619 ER PT J AU Morelli, TL Daly, C Dobrowski, SZ Dulen, DM Ebersole, JL Jackson, ST Lundquist, JD Millar, CI Maher, SP Monahan, WB Nydick, KR Redmond, KT Sawyer, SC Stock, S Beissinger, SR AF Morelli, Toni Lyn Daly, Christopher Dobrowski, Solomon Z. Dulen, Deanna M. Ebersole, Joseph L. Jackson, Stephen T. Lundquist, Jessica D. Millar, Constance I. Maher, Sean P. Monahan, William B. Nydick, Koren R. Redmond, Kelly T. Sawyer, Sarah C. Stock, Sarah Beissinger, Steven R. TI Managing Climate Change Refugia for Climate Adaptation SO PLOS ONE LA English DT Article ID UNITED-STATES; RIVER RESTORATION; CHANGE IMPACTS; BIODIVERSITY; MICROREFUGIA; ECOSYSTEMS; MODELS; CONSERVATION; COMMUNITIES; RESPONSES AB Refugia have long been studied from paleontological and biogeographical perspectives to understand how populations persisted during past periods of unfavorable climate. Recently, researchers have applied the idea to contemporary landscapes to identify climate change refugia, here defined as areas relatively buffered from contemporary climate change over time that enable persistence of valued physical, ecological, and socio-cultural resources. We differentiate historical and contemporary views, and characterize physical and ecological processes that create and maintain climate change refugia. We then delineate how refugia can fit into existing decision support frameworks for climate adaptation and describe seven steps for managing them. Finally, we identify challenges and opportunities for operationalizing the concept of climate change refugia. Managing climate change refugia can be an important option for conservation in the face of ongoing climate change. C1 [Morelli, Toni Lyn] US Geol Survey, DOI Northeast Climate Sci Ctr, Amherst, MA 01002 USA. [Morelli, Toni Lyn; Maher, Sean P.; Beissinger, Steven R.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Morelli, Toni Lyn; Maher, Sean P.; Beissinger, Steven R.] Univ Calif Berkeley, Museum Vertebrate Zool, Berkeley, CA 94720 USA. [Daly, Christopher] Oregon State Univ, Coll Engn, Corvallis, OR 97331 USA. [Dobrowski, Solomon Z.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Dulen, Deanna M.] Natl Pk Serv, Devils Postpile Natl Monument, Mammoth Lakes, CA 93546 USA. [Ebersole, Joseph L.] US EPA, Western Ecol Div, Corvallis, OR 97333 USA. [Jackson, Stephen T.] US Geol Survey, DOI Southwest Climate Sci Ctr, Tucson, AZ 85721 USA. [Jackson, Stephen T.] Univ Arizona, Dept Geosci, Tucson, AZ 85721 USA. [Jackson, Stephen T.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Lundquist, Jessica D.] Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA. [Millar, Constance I.] USDA Forest Serv, Pacific Southwest Res Stn, Albany, CA 94710 USA. [Maher, Sean P.] Missouri State Univ, Dept Biol, Springfield, MO 65897 USA. [Monahan, William B.] USDA Forest Serv, Forest Hlth Technol Enterprise Team, Ft Collins, CO 80526 USA. [Nydick, Koren R.] Natl Pk Serv, Sequoia & Kings Canyon Natl Pk, Three Rivers, CA USA. [Redmond, Kelly T.] Desert Res Inst, Western Reg Climate Ctr, Reno, NV 89506 USA. [Sawyer, Sarah C.] USDA Forest Serv, Pacific Southwest Reg, Vallejo, CA 94592 USA. [Stock, Sarah] Natl Pk Serv, Yosemite Natl Pk, El Portal, CA 95318 USA. RP Morelli, TL (reprint author), US Geol Survey, DOI Northeast Climate Sci Ctr, Amherst, MA 01002 USA.; Morelli, TL (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA.; Morelli, TL (reprint author), Univ Calif Berkeley, Museum Vertebrate Zool, Berkeley, CA 94720 USA. EM tmorelli@usgs.gov OI Morelli, Toni Lyn/0000-0001-5865-5294 FU California Landscape Conservation Cooperative; Department of Interior Northeast Climate Science Center FX This work was supported by funding from the California Landscape Conservation Cooperative (www.californialcc.org) and the Department of Interior Northeast Climate Science Center (necsc.umass.edu). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 76 TC 5 Z9 5 U1 27 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 10 PY 2016 VL 11 IS 8 AR e0159909 DI 10.1371/journal.pone.0159909 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT3KR UT WOS:000381380400017 PM 27509088 ER PT J AU Kim, SY Dutton, SJ Sheppard, L Hannigan, MP Miller, SL Milford, JB Peel, JL Vedal, S AF Kim, Sun-Young Dutton, Steven J. Sheppard, Lianne Hannigan, Michael P. Miller, Shelly L. Milford, Jana B. Peel, Jennifer L. Vedal, Sverre TI The short-term association of selected components of fine particulate matter and mortality in the Denver Aerosol Sources and Health (DASH) study (vol 14, 49, 2015) SO ENVIRONMENTAL HEALTH LA English DT Article C1 [Kim, Sun-Young; Sheppard, Lianne; Vedal, Sverre] Univ Washington, Sch Publ Hlth, Dept Environm & Occupat Hlth Sci, Seattle, WA 98195 USA. [Kim, Sun-Young] Seoul Natl Univ, Inst Healthand Environm, Seoul, South Korea. [Dutton, Steven J.] US EPA, Natl Ctr Environm Assessment, Res Triangle Pk, NC USA. [Sheppard, Lianne] Univ Washington, Sch Publ Hlth, Dept Biostat, Seattle, WA 98195 USA. [Hannigan, Michael P.; Miller, Shelly L.; Milford, Jana B.] Univ Colorado, Dept Mech Engn, Coll Engn & Appl Sci, Boulder, CO 80309 USA. [Peel, Jennifer L.] Colorado State Univ, Dept Environm & Radiol Hlth Sci, Ft Collins, CO 80523 USA. RP Kim, SY (reprint author), Univ Washington, Sch Publ Hlth, Dept Environm & Occupat Hlth Sci, Seattle, WA 98195 USA.; Kim, SY (reprint author), Seoul Natl Univ, Inst Healthand Environm, Seoul, South Korea. EM puha0@uw.edu FU National Institute of Environmental Health Sciences [R01 ES010197]; U.S. Environmental Protection Agency [RD 834796]; National Research Foundation of Korea (Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education) [2013R1A6A3A04059017] FX This work was supported by the National Institute of Environmental Health Sciences (R01 ES010197), the U.S. Environmental Protection Agency (RD 834796), and the National Research Foundation of Korea (Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education: 2013R1A6A3A04059017). NR 1 TC 0 Z9 0 U1 1 U2 1 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1476-069X J9 ENVIRON HEALTH-GLOB JI Environ. Health PD AUG 9 PY 2016 VL 15 AR 85 DI 10.1186/s12940-016-0169-1 PG 1 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DT6BP UT WOS:000381568100001 ER PT J AU Ollson, CJ Smith, E Scheckel, KG Betts, AR Juhasz, AL AF Ollson, Cameron J. Smith, Euan Scheckel, Kirk G. Betts, Aaron R. Juhasz, Albert L. TI Assessment of arsenic speciation and bioaccessibility in mine-impacted materials SO JOURNAL OF HAZARDOUS MATERIALS LA English DT Article DE Bioaccessibility; Arsenic; Tailings; Calcinate; Slime ID VITRO DIGESTION MODELS; CONTAMINATED SOILS; PARTICLE-SIZE; RELATIVE BIOAVAILABILITY; GOLD MINE; RISK-ASSESSMENT; NOVA-SCOTIA; IN-VIVO; TAILINGS; SPECTROSCOPY AB Mine-impacted materials were collected from Victoria, Australia and categorized into three source materials; tailings (n = 35), calcinated (n = 10) and grey slimes (n = 5). Arsenic (As) concentrations in these materials varied over several orders of magnitude (30-47,000 mg kg(-1)), with median concentrations of 500, 10,800 and 1500 mg kg(-1), respectively. When As bioaccessibility was assessed using the Solubility Bioaccessibility Research Consortium (SBRC) assay, As bioaccessibility ranged between 4 and 90%, with mean gastric phase values of 30%, 49% and 82% for tailings, calcinated and grey slimes, respectively. An analysis of variance (ANOVA) determined that As bioaccessibility was significantly different (P<0.05) between source materials. This was due to differences in As mineralogy, soil particle size as well as the concentration and nature of Fe present. X-ray Absorption Near Edge Structure (XANES) analysis identified arseniosiderite, yukonite, realgar, loellingite and mineral sorbed arsenate species in mine-impacted materials. Despite differences in physicochemical properties, 'mine wastes' are often reported under a generic descriptor. Outcomes from this research highlight that variability in As bioaccessibility can be prescribed to As mineralogy and matrix physicochemical properties, while categorizing samples into sub-groups can provide some notional indication of potential exposure. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ollson, Cameron J.; Smith, Euan; Juhasz, Albert L.] Univ S Australia, Future Ind Inst, Mawson Lakes, SA 5095, Australia. [Scheckel, Kirk G.; Betts, Aaron R.] US EPA, Natl Risk Management Res Lab, Land Remediat & Pollut Control Div, Cincinnati, OH 45224 USA. RP Ollson, CJ (reprint author), Univ S Australia, Future Ind Inst, Mawson Lakes, SA 5095, Australia. EM cameron.ollson@mymail.unisa.edu.au RI BM, MRCAT/G-7576-2011; Juhasz, Albert/F-6600-2011; OI Juhasz, Albert/0000-0002-1164-4085; Scheckel, Kirk/0000-0001-9326-9241 FU Future Industries Institute, University of South Australia; Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC CARE) [3.1.07.11/12]; Department of Energy; MRCAT; DOE Office of Science by Argonne National Laboratory [DE-AC02-06CH11357]; EPA FX The authors acknowledge the support of the Future Industries Institute, University of South Australia and the Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC CARE) (Project 3.1.07.11/12). XANES analysis was performed at the Advanced Photon Source (APS), Argonne, Illinois, USA. MRCAT operations are supported by the Department of Energy and the MRCAT member institutions. This research used resources of the APS, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. Although EPA contributed to this article, the research presented was not performed by or funded by EPA and was not subject to EPA's quality system requirements. Consequently, the views, interpretations, and conclusions expressed in this article are solely those of the authors and do not necessarily reflect or represent EPA's views or policies. NR 41 TC 0 Z9 0 U1 24 U2 66 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3894 EI 1873-3336 J9 J HAZARD MATER JI J. Hazard. Mater. PD AUG 5 PY 2016 VL 313 BP 130 EP 137 DI 10.1016/j.jhazmat.2016.03.090 PG 8 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DN1FF UT WOS:000376811000014 PM 27060218 ER PT J AU Wang, LL Han, C Nadagouda, MN Dionysiou, DD AF Wang, Lingling Han, Changseok Nadagouda, Mallikarjuna N. Dionysiou, Dionysios D. TI An innovative zinc oxide-coated zeolite adsorbent for removal of humic acid SO JOURNAL OF HAZARDOUS MATERIALS LA English DT Article DE Electrostatic interaction; Humic acid; Water treatment; Zeolite; Zinc oxide; Natural organic matter ID NATURAL ORGANIC-MATTER; AQUEOUS-SOLUTION; ACTIVATED CARBON; ADSORPTION; WATER; IONS; CHARGE; MONTMORILLONITE; ULTRAFILTRATION; EQUILIBRIUM AB Zinc oxide (ZnO)-coated zeolite adsorbents were developed by both nitric acid modification and Zn(NO3)(2)center dot 6H(2)O functionalization of zeolite 4A. The developed adsorbents were used for the removal of humic acid (HA) from aqueous solutions. The synthesized materials were characterized by porosimetry analysis, scanning electron microscopy, X-Ray diffraction analysis, and high resolution transmission electron microscopy. The maximum adsorption capacity of the adsorbents at 21 +/- 1 degrees C was about 60 mgCg(-1). The results showed that the positive charge density of ZnO-coated zeolite adsorbents was proportional to the amount of ZnO coated on zeolite and thus, ZnO-coated zeolite adsorbents exhibited a greater affinity for negatively charged ions. Furthermore, the adsorption capacity of ZnO-coated zeolite adsorbents increased markedly after acid modification. Adsorption experiments demonstrated ZnO-coated zeolite adsorbents possessed high adsorption capacity to remove HA from aqueous solutions mainly due C1 [Wang, Lingling] Quanzhou Normal Univ, Coll Chem Engn & Mat, Quanzhou 362000, Fujian, Peoples R China. [Wang, Lingling; Dionysiou, Dionysios D.] Univ Cincinnati, Engn Res Ctr 705, Environm Engn & Sci Program, Cincinnati, OH 45221 USA. [Han, Changseok] US EPA, ORD, NRMRL, STD,CPB, 26 W Martin Luther King Jr Dr, Cincinnati, OH 45268 USA. [Nadagouda, Mallikarjuna N.] US EPA, ORD, NRMRL, WSWRD,WQMB, 26 W Martin Luther King Jr Dr, Cincinnati, OH 45268 USA. [Dionysiou, Dionysios D.] Univ Cyprus, Sch Engn, Nireas Int Water Res Ctr, POB 20537, CY-1678 Nicosia, Cyprus. RP Wang, LL (reprint author), Quanzhou Normal Univ, Coll Chem Engn & Mat, Quanzhou 362000, Fujian, Peoples R China.; Dionysiou, DD (reprint author), Univ Cincinnati, Engn Res Ctr 705, Environm Engn & Sci Program, Cincinnati, OH 45221 USA. EM lasier_wang@hotmail.com; dionysios.d.dionysiou@uc.edu FU Natural Science Foundation of Fujian Province, P.R. China [2014J01055]; Cyprus Research Promotion Foundation - European Regional Development Fund; Republic of Cyprus (Strategic Infrastructure Project NEA) [YPiODeltaOMH/SigmaTPATH/0308/09]; National Risk Management Research Laboratory FX The work was financially supported by the the Natural Science Foundation of Fujian Province, P.R. China (No. 2014J01055), and partially funded by the Cyprus Research Promotion Foundation through Desmi 2009-2010 which was co-financed by the European Regional Development Fund and the Republic of Cyprus (Strategic Infrastructure Project NEA Upsilon Pi O Delta OMH/Sigma TPATH/0308/09). C. Han was supported by the Postgraduate Research Program at the National Risk Management Research Laboratory administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Environmental Protection Agency. NR 34 TC 4 Z9 4 U1 19 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3894 EI 1873-3336 J9 J HAZARD MATER JI J. Hazard. Mater. PD AUG 5 PY 2016 VL 313 BP 283 EP 290 DI 10.1016/j.jhazmat.2016.03.070 PG 8 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DN1FF UT WOS:000376811000031 PM 27135170 ER PT J AU Li, Y Schichtel, BA Walker, JT Schwede, DB Chen, X Lehmann, CMB Puchalski, MA Gay, DA Collett, JL AF Li, Yi Schichtel, Bret A. Walker, John T. Schwede, Donna B. Chen, Xi Lehmann, Christopher M. B. Puchalski, Melissa A. Gay, David A. Collett, Jeffrey L., Jr. TI REPLY TO SUN ET AL.: Deposition of organic nitrogen SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Letter C1 [Li, Yi; Collett, Jeffrey L., Jr.] Colorado State Univ, Dept Atmospher Sci, Ft Collins, CO 80523 USA. [Schichtel, Bret A.] Colorado State Univ, Natl Pk Serv, Cooperat Inst Res Atmosphere, Ft Collins, CO 80523 USA. [Walker, John T.; Chen, Xi] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Schwede, Donna B.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Lehmann, Christopher M. B.; Gay, David A.] Univ Illinois, Natl Atmospher Deposit Program, Champaign, IL 61820 USA. [Puchalski, Melissa A.] US EPA, Clean Air Markets Div, Washington, DC 20460 USA. RP Collett, JL (reprint author), Colorado State Univ, Dept Atmospher Sci, Ft Collins, CO 80523 USA. EM collett@atmos.colostate.edu RI Collett, Jeffrey/F-2862-2010 OI Collett, Jeffrey/0000-0001-9180-508X NR 4 TC 0 Z9 0 U1 7 U2 11 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD AUG 2 PY 2016 VL 113 IS 31 BP E4435 EP E4435 DI 10.1073/pnas.1608932113 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS2HH UT WOS:000380586600002 PM 27439877 ER PT J AU Ganguli, AC Engle, DM Mayer, PM Salo, LF AF Ganguli, A. C. Engle, D. M. Mayer, P. M. Salo, L. F. TI Influence of resource availability on Juniperus virginiana expansion in a forest-prairie ecotone SO ECOSPHERE LA English DT Article DE cross timbers; eastern redcedar; invasion; seedlings; soil texture; tallgrass prairie; upland oak forest; woody plant expansion ID RED CEDAR JUNIPERUS; SOUTHERN GREAT-PLAINS; XERIC LIMESTONE PRAIRIES; TALLGRASS PRAIRIE; EASTERN REDCEDAR; UNITED-STATES; HERBACEOUS VEGETATION; SEMIARID GRASSLANDS; SEEDLING SURVIVAL; INVASION ECOLOGY AB Woody plant expansion into grasslands and savannas is a global concern. Rapid expansion of Juniperus virginiana, a tree native to North America, has profound ecological consequences. We used transplanted J. virginiana seedlings to investigate the role of resource availability on J. virginiana expansion following the removal of fire, the factor historically limiting range expansion of this fire-intolerant species. We evaluated J. virginiana seedling survival and seedling growth, two important phases in woody plant expansion, relative to two belowground resource factors, plant-available soil water (soil clay content, an index of plant-available soil water) and plant-available nitrogen (PAN), and an aboveground factor, photosynthetic active radiation (PAR). In three plant communities associated with an oak forest-tallgrass prairie ecotone, we transplanted 2-yr-old J. virginiana seedlings in a systematic grid design and measured J. virginiana seedling survival and growth 8, 20, and 30 months following the transplant. We also measured soil clay content, PAN, and PAR in 1-m(2) quadrats centered on each transplanted seedling. We employed path analysis at two spatial scales (144 and 2916 m(2)) to compare the role of resource factors in seedling growth and survival. Juniperus virginiana seedling survival was about 10% greater in tallgrass prairie and upland oak forest than in the old field, and seedling growth in tallgrass prairie exceeded the two other communities by about a factor of five. Tallgrass prairie in our study area is clearly more vulnerable to expansion of J. virginiana than the other two plant communities. Survival and growth were controlled largely by available light (PAR) and secondarily by plant-available soil water, indexed by soil clay content. In all three vegetation types and at both fine and coarse scales, soil clay content also exerted an indirect effect, possibly by mitigating the influence of light. Results of this study suggest that soil distribution maps and associated ecological site designations might be useful for directing J. virginiana management in the oak forest-tallgrass prairie ecotone by identifying the potential hotspots of invasion. C1 [Ganguli, A. C.; Engle, D. M.] Oklahoma State Univ, Dept Nat Resource Ecol & Management, Stillwater, OK 74078 USA. [Mayer, P. M.] US EPA, Western Ecol Div, Natl Hlth & Environm Effects Res Lab, Corvallis, OR 97333 USA. [Salo, L. F.] Sage Ecosyst Sci, Boise, ID 83707 USA. [Ganguli, A. C.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. RP Ganguli, AC (reprint author), Oklahoma State Univ, Dept Nat Resource Ecol & Management, Stillwater, OK 74078 USA.; Ganguli, AC (reprint author), New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. EM ganguli@nmsu.edu RI Ganguli, Amy/J-3342-2014 OI Ganguli, Amy/0000-0003-3960-1404 FU U.S. Environmental Protection Agency (Agency) through its Office of Research and Development [DW-14-93900001-1]; Biological Resources Division of the United States Geological Survey (USGS); Oklahoma Agricultural Experiment Station FX The U.S. Environmental Protection Agency (Agency) through its Office of Research and Development partially funded and collaborated in the research described here under an interagency agreement (DW-14-93900001-1) with the Biological Resources Division of the United States Geological Survey (USGS), administered by the Oklahoma Cooperative Fish and Wildlife Research Unit located at Oklahoma State University (U.S. Geological Survey [USGS], Oklahoma State University, Oklahoma Department of Wildlife Conservation, and Wildlife Management Institute cooperating). The information in this document has been subjected to agency peer and administrative review, and it has been approved for publication as an agency document. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. The Oklahoma Agricultural Experiment Station also provided support to the first two authors. This article is published with the approval of the director, Oklahoma Agricultural Experiment Station. We thank D. M. Leslie Jr. and E. C. Hellgren for their contributions, the many field technicians who assisted with this work, and Ryan Limb for reviewing an earlier version of this manuscript. NR 98 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD AUG PY 2016 VL 7 IS 8 AR e01433 DI 10.1002/ecs2.1433 PG 15 WC Ecology SC Environmental Sciences & Ecology GA EB2RG UT WOS:000387208900024 ER PT J AU Blodgett, D Read, E Lucido, J Slawecki, T Young, D AF Blodgett, David Read, Emily Lucido, Jessica Slawecki, Tad Young, Dwane TI AN ANALYSIS OF WATER DATA SYSTEMS TO INFORM THE OPEN WATER DATA INITIATIVE SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE data management; public participation; hydrologic cycle; geospatial analysis; open data; network linked asset AB Improving access to data and fostering open exchange of water information is foundational to solving water resources issues. In this vein, the Department of the Interior's Assistant Secretary for Water and Science put forward the charge to undertake an Open Water Data Initiative (OWDI) that would prioritize and accelerate work toward better water data infrastructure. The goal of the OWDI is to build out the Open Water Web (OWW). We therefore considered the OWW in terms of four conceptual functions: water data cataloging, water data as a service, enriching water data, and community for water data. To describe the current state of the OWW and identify areas needing improvement, we conducted an analysis of existing systems using a standard model for describing distributed systems and their business requirements. Our analysis considered three OWDI-focused use cases-flooding, drought, and contaminant transport-and then examined the landscape of other existing applications that support the Open Water Web. The analysis, which includes a discussion of observed successful practices of cataloging, serving, enriching, and building community around water resources data, demonstrates that we have made significant progress toward the needed infrastructure, although challenges remain. The further development of the OWW can be greatly informed by the interpretation and findings of our analysis. C1 [Blodgett, David; Read, Emily; Lucido, Jessica] US Geol Survey, Off Water Informat, 8505 Res Way, Middleton, WI 53562 USA. [Slawecki, Tad] LimnoTech, Ann Arbor, MI 48108 USA. [Young, Dwane] US EPA, Off Water, Washington, DC 20460 USA. RP Blodgett, D (reprint author), US Geol Survey, Off Water Informat, 8505 Res Way, Middleton, WI 53562 USA. EM dblodgett@usgs.gov OI Blodgett, David/0000-0001-9489-1710; Read, Emily/0000-0002-9617-9433 NR 12 TC 1 Z9 1 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD AUG PY 2016 VL 52 IS 4 BP 845 EP 858 DI 10.1111/1752-1688.12417 PG 14 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EB2DW UT WOS:000387168800005 ER PT J AU Larsen, S Hamilton, S Lucido, J Garner, B Young, D AF Larsen, Sara Hamilton, Stuart Lucido, Jessica Garner, Bradley Young, Dwane TI SUPPORTING DIVERSE DATA PROVIDERS IN THE OPEN WATER DATA INITIATIVE: COMMUNICATING WATER DATA QUALITY AND FITNESS OF USE SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE planning; public participation; data management; quality assurance/quality control (QAQC); water data; hydrology; water quality; groundwater AB Shared, trusted, timely data are essential elements for the cooperation needed to optimize economic, ecologic, and public safety concerns related to water. The Open Water Data Initiative (OWDI) will provide a fully scalable platform that can support a wide variety of data from many diverse providers. Many of these will be larger, well-established, and trusted agencies with a history of providing well-documented, standardized, and archive-ready products. However, some potential partners may be smaller, distributed, and relatively unknown or untested as data providers. The data these partners will provide are valuable and can be used to fill in many data gaps, but can also be variable in quality or supplied in nonstandardized formats. They may also reflect the smaller partners' variable budgets and missions, be intermittent, or of unknown provenance. A challenge for the OWDI will be to convey the quality and the contextual "fitness" of data from providers other than the most trusted brands. This article reviews past and current methods for documenting data quality. Three case studies are provided that describe processes and pathways for effective data-sharing and publication initiatives. They also illustrate how partners may work together to find a metadata reporting threshold that encourages participation while maintaining high data integrity. And lastly, potential governance is proposed that may assist smaller partners with short-and long-term participation in the OWDI. C1 [Larsen, Sara] Western States Water Council, 5296 Commerce Dr,Ste 202, Murray, UT 84107 USA. [Hamilton, Stuart] Aquat Informat, Vancouver, BC V6E 4M3, Canada. [Lucido, Jessica] US Geol Survey, Ctr Integrated Data Analyt, Middleton, WI 53562 USA. [Garner, Bradley] US Geol Survey, Water Data & Serv Nation, Flagstaff, AZ 86001 USA. [Young, Dwane] US EPA, Off Water, Washington, DC 20460 USA. RP Larsen, S (reprint author), Western States Water Council, 5296 Commerce Dr,Ste 202, Murray, UT 84107 USA. EM saralarsen@wswc.utah.gov NR 19 TC 1 Z9 1 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD AUG PY 2016 VL 52 IS 4 BP 859 EP 872 DI 10.1111/1752-1688.12406 PG 14 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EB2DW UT WOS:000387168800006 ER PT J AU Moore, RB Dewald, TG AF Moore, Richard B. Dewald, Thomas G. TI THE ROAD TO NHDPLUS - ADVANCEMENTS IN DIGITAL STREAM NETWORKS AND ASSOCIATED CATCHMENTS SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE river networks; watersheds; geospatial analysis; rivers/streams ID UNITED-STATES AB A progression of advancements in Geographic Information Systems techniques for hydrologic network and associated catchment delineation has led to the production of the National Hydrography Dataset Plus (NHDPlus). NHDPlus is a digital stream network for hydrologic modeling with catchments and a suite of related geospatial data. Digital stream networks with associated catchments provide a geospatial framework for linking and integrating water-related data. Advancements in the development of NHDPlus are expected to continue to improve the capabilities of this national geospatial hydrologic framework. NHDPlus is built upon the medium-resolution NHD and, like NHD, was developed by the U.S. Environmental Protection Agency and U.S. Geological Survey to support the estimation of streamflow and stream velocity used in fate-and-transport modeling. Catchments included with NHDPlus were created by integrating vector information from the NHD and from the Watershed Boundary Dataset with the gridded land surface elevation as represented by the National Elevation Dataset. NHDPlus is an actively used and continually improved dataset. Users recognize the importance of a reliable stream network and associated catchments. The NHDPlus spatial features and associated data tables will continue to be improved to support regional water quality and streamflow models and other user-defined applications. C1 [Moore, Richard B.] US Geol Survey, New England Water Sci Ctr, Pembroke, NH 03275 USA. [Dewald, Thomas G.] US EPA, Off Wetlands Oceans & Watersheds, Washington, DC 20460 USA. RP Moore, RB (reprint author), US Geol Survey, New England Water Sci Ctr, Pembroke, NH 03275 USA. EM rmoore@usgs.gov NR 26 TC 4 Z9 4 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD AUG PY 2016 VL 52 IS 4 BP 890 EP 900 DI 10.1111/1752-1688.12389 PG 11 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EB2DW UT WOS:000387168800008 ER PT J AU Pellerin, BA Stauffer, BA Young, DA Sullivan, DJ Bricker, SB Walbridge, MR Clyde, GA Shaw, DM AF Pellerin, Brian A. Stauffer, Beth A. Young, Dwane A. Sullivan, Daniel J. Bricker, Suzanne B. Walbridge, Mark R. Clyde, Gerard A., Jr. Shaw, Denice M. TI EMERGING TOOLS FOR CONTINUOUS NUTRIENT MONITORING NETWORKS: SENSORS ADVANCING SCIENCE AND WATER RESOURCES PROTECTION SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE sensors; nutrients; water quality; information management ID HARMFUL ALGAL BLOOMS; SPRING-FED RIVER; IN-SITU SENSORS; MISSISSIPPI RIVER; HIGH-RESOLUTION; DRINKING-WATER; WASTE-WATER; DATA DELUGE; NITRATE; FREQUENCY AB Sensors and enabling technologies are becoming increasingly important tools for water quality monitoring and associated water resource management decisions. In particular, nutrient sensors are of interest because of the well-known adverse effects of nutrient enrichment on coastal hypoxia, harmful algal blooms, and impacts to human health. Accurate and timely information on nutrient concentrations and loads is integral to strategies designed to minimize risk to humans and manage the underlying drivers of water quality impairment. Using nitrate sensors as the primary example, we highlight the types of applications in freshwater and coastal environments that are likely to benefit from continuous, real-time nutrient data. The concurrent emergence of new tools to integrate, manage, and share large datasets is critical to the successful use of nutrient sensors and has made it possible for the field of continuous monitoring to rapidly move forward. We highlight several near-term opportunities for federal agencies, as well as the broader scientific and management community, that will help accelerate sensor development, build and leverage sites within a national network, and develop open data standards and data management protocols that are key to realizing the benefits of a large-scale, integrated monitoring network. Investing in these opportunities will provide new information to guide management and policies designed to protect and restore our nation's water resources. C1 [Pellerin, Brian A.] US Geol Survey, Calif Water Sci Ctr, 6000 J St,Placer Hall, Sacramento, CA 95819 USA. [Stauffer, Beth A.] Univ Louisiana Lafayette, Dept Biol, Lafayette, LA 70504 USA. [Young, Dwane A.] US EPA, Off Water, Washington, DC 20460 USA. [Sullivan, Daniel J.] US Geol Survey, Wisconsin Water Sci Ctr, Middleton, WI 53562 USA. [Bricker, Suzanne B.] NOAA, Natl Ctr Coastal Ocean Sci, Silver Spring, MD 20910 USA. [Walbridge, Mark R.] ARS, USDA, Beltsville, MD 20705 USA. [Clyde, Gerard A., Jr.] US Army Corp Engineers, Tulsa Dist, Tulsa, OK 74128 USA. [Shaw, Denice M.] US EPA, Off Res & Dev, Washington, DC 20460 USA. RP Pellerin, BA (reprint author), US Geol Survey, Calif Water Sci Ctr, 6000 J St,Placer Hall, Sacramento, CA 95819 USA. EM bpeller@usgs.gov FU USGS Office of Water Quality; USGS National Water Quality Assessment Program; National Water Quality Monitoring Council FX The authors benefited from insights and comments from Robert Gilliom, Charles Crawford, Donna Myers, Bill Wilber, Gary Rowe, Pixie Hamilton, Mark Nilles, Andy Ziegler, Jeff Lape, and Richard Mitchell. We thank Pete Penoyer (National Park Service) and two anonymous reviewers for helpful comments on the manuscript. This article was supported by the USGS Office of Water Quality, the USGS National Water Quality Assessment Program, and the National Water Quality Monitoring Council. NR 81 TC 1 Z9 1 U1 11 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD AUG PY 2016 VL 52 IS 4 BP 993 EP 1008 DI 10.1111/1752-1688.12386 PG 16 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EB2DW UT WOS:000387168800016 ER PT J AU Fennel, K Laurent, A Hetland, R Justic, D Ko, DS Lehrter, J Murrell, M Wang, LX Yu, LQ Zhang, WX AF Fennel, Katja Laurent, Arnaud Hetland, Robert Justic, Dubravko Ko, Dong S. Lehrter, John Murrell, Michael Wang, Lixia Yu, Liuqian Zhang, Wenxia TI Effects of model physics on hypoxia simulations for the northern Gulf of Mexico: A model intercomparison SO JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS LA English DT Article ID LOUISIANA CONTINENTAL-SHELF; UNSTRUCTURED GRIDS; OXYGEN-CONSUMPTION; CHESAPEAKE BAY; OCEAN; WIND; VARIABILITY; SEDIMENT; COASTAL; VOLUME AB A large hypoxic zone forms every summer on the Texas-Louisiana Shelf in the northern Gulf of Mexico due to nutrient and freshwater inputs from the Mississippi/Atchafalaya River System. Efforts are underway to reduce the extent of hypoxic conditions through reductions in river nutrient inputs, but the response of hypoxia to such nutrient load reductions is difficult to predict because biological responses are confounded by variability in physical processes. The objective of this study is to identify the major physical model aspects that matter for hypoxia simulation and prediction. In order to do so, we compare three different circulation models (ROMS, FVCOM, and NCOM) implemented for the northern Gulf of Mexico, all coupled to the same simple oxygen model, with observations and against each other. By using a highly simplified oxygen model, we eliminate the potentially confounding effects of a full biogeochemical model and can isolate the effects of physical features. In a systematic assessment, we found that (1) model-tomodel differences in bottom water temperatures result in differences in simulated hypoxia because temperature influences the uptake rate of oxygen by the sediments (an important oxygen sink in this system), (2) vertical stratification does not explain model-to-model differences in hypoxic conditions in a straightforward way, and (3) the thickness of the bottom boundary layer, which sets the thickness of the hypoxic layer in all three models, is key to determining the likelihood of a model to generate hypoxic conditions. These results imply that hypoxic area, the commonly used metric in the northern Gulf which ignores hypoxic layer thickness, is insufficient for assessing a model's ability to accurately simulate hypoxia, and that hypoxic volume needs to be considered as well. C1 [Fennel, Katja; Laurent, Arnaud; Yu, Liuqian; Zhang, Wenxia] Dalhousie Univ, Dept Oceanog, Halifax, NS, Canada. [Hetland, Robert; Zhang, Wenxia] Texas A&M Univ, Dept Oceanog, College Stn, TX 77843 USA. [Justic, Dubravko; Wang, Lixia] Louisiana State Univ, Dept Oceanog & Coastal Sci, Baton Rouge, LA 70803 USA. [Ko, Dong S.] Naval Res Lab, Div Oceanog, Hancock Cty, MS USA. [Lehrter, John; Murrell, Michael] US EPA, Gulf Ecol Div, Gulf Breeze, FL USA. RP Fennel, K (reprint author), Dalhousie Univ, Dept Oceanog, Halifax, NS, Canada. EM Katja.Fennel@dal.ca OI Laurent, Arnaud/0000-0002-8545-9309 FU NOAA through Coastal Ocean Modeling Testbed (COMT) project FX This work was supported by NOAA through the Coastal Ocean Modeling Testbed (COMT) project. All data used in this publication are available via the links provided in the methods section. The views expressed in this manuscript are those of the authors and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency. NGOMEX publication number 210. NR 53 TC 2 Z9 2 U1 3 U2 3 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-9275 EI 2169-9291 J9 J GEOPHYS RES-OCEANS JI J. Geophys. Res.-Oceans PD AUG PY 2016 VL 121 IS 8 BP 5731 EP 5750 DI 10.1002/2015JC011577 PG 20 WC Oceanography SC Oceanography GA EA8TU UT WOS:000386912700021 ER PT J AU Beck, MW AF Beck, Marcus W. TI SWMPr: An R Package for Retrieving, Organizing, and Analyzing Environmental Data for Estuaries SO R JOURNAL LA English DT Article AB The System-Wide Monitoring Program (SWMP) was implemented in 1995 by the US National Estuarine Research Reserve System. This program has provided two decades of continuous monitoring data at over 140 fixed stations in 28 estuaries. However, the increasing quantity of data provided by the monitoring network has complicated broad-scale comparisons between systems and, in some cases, prevented simple trend analysis of water quality parameters at individual sites. This article describes the SWMPr package that provides several functions that facilitate data retrieval, organization, and analysis of time series data in the reserve estuaries. Previously unavailable functions for estuaries are also provided to estimate rates of ecosystem metabolism using the open-water method. The SWMPr package has facilitated a cross-reserve comparison of water quality trends and links quantitative information with analysis tools that have use for more generic applications to environmental time series. C1 [Beck, Marcus W.] US EPA, Gulf Ecol Div, Natl Hlth & Environm Effects Res Lab, 1 Sabine Isl Dr, Gulf Breeze, FL 32651 USA. RP Beck, MW (reprint author), US EPA, Gulf Ecol Div, Natl Hlth & Environm Effects Res Lab, 1 Sabine Isl Dr, Gulf Breeze, FL 32651 USA. EM beck.marcus@epa.gov NR 17 TC 0 Z9 0 U1 1 U2 1 PU R FOUNDATION STATISTICAL COMPUTING PI WIEN PA WIRTSCHAFTSUNIVERSITAT, INST STATISTICS & MATHEMATICS, AUGASSE 2-6, WIEN, 1090, AUSTRIA SN 2073-4859 J9 R J JI R Journal PD AUG PY 2016 VL 8 IS 1 BP 219 EP 232 PG 14 WC Computer Science, Interdisciplinary Applications; Statistics & Probability SC Computer Science; Mathematics GA DY6YP UT WOS:000385276100016 ER PT J AU Harrill, AH McCullough, SD Wood, CE Kahle, JJ Chorley, BN AF Harrill, Alison H. McCullough, Shaun D. Wood, Charles E. Kahle, Juliette J. Chorley, Brian N. TI MicroRNA Biomarkers of Toxicity in Biological Matrices SO TOXICOLOGICAL SCIENCES LA English DT Review DE microRNA; biomarkers; liquid biopsy; tissue injury and toxicity; accessible matrices ID ACUTE MYOCARDIAL-INFARCTION; INDUCED LIVER-INJURY; CIRCULATING MICRORNAS; PANCREATIC INJURY; EXTRACELLULAR MICRORNAS; POTENTIAL BIOMARKERS; HEALTHY-VOLUNTEERS; SAFETY ASSESSMENT; PLASMA MICRORNAS; TUBULAR INJURY AB Biomarker measurements that reliably correlate with tissue injury and that can be measured within accessible biofluids offer benefits in terms of cost, time, and convenience when assessing chemical and drug-induced toxicity in model systems or human cohorts. MicroRNAs (miRNAs) have emerged in recent years as a promising new class of biomarker for monitoring toxicity. Recent enthusiasm for miRNA biomarker research has been fueled by evidence that certain miRNAs are cell-type specific and are released during injury, thus raising the possibility of using biofluid-based miRNAs as a "liquid biopsy" that may be obtained by sampling extracellular fluids. As biomarkers, miRNAs demonstrate improved stability as compared with many protein markers and sequences are largely conserved across species, simplifying analytical techniques. Recent efforts have sought to identify miRNAs that are released into accessible biofluids following xenobiotic exposure, using compounds that target specific organs. Whereas still early in the discovery phase, miRNA biomarkers will have an increasingly important role in the assessment of adverse effects of both environmental chemicals and pharmaceutical drugs. Here, we review the current findings of biofluid-based miRNAs, as well as highlight technical challenges in assessing toxicologic pathology using these biomarkers. C1 [Harrill, Alison H.; Chorley, Brian N.] Univ Arkansas Med Sci, Dept Environm & Occupat Hlth, Little Rock, AR 72205 USA. [McCullough, Shaun D.; Wood, Charles E.; Kahle, Juliette J.] US EPA, ORD, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27709 USA. [Kahle, Juliette J.] Counsyl Inc, 180 Kimball Way, San Francisco, CA 94080 USA. RP Chorley, BN (reprint author), Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr,Mail Drop B-105-03, Res Triangle Pk, NC 27711 USA. EM chorley.brian@epa.gov FU U.S. Environmental Protection Agency, Office of Research and Development; Burroughs Wellcome Fund Innovation in Regulatory Science Award FX Funding for this study came from the U.S. Environmental Protection Agency, Office of Research and Development. A.H.H. is supported by the Burroughs Wellcome Fund Innovation in Regulatory Science Award. NR 77 TC 2 Z9 2 U1 3 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD AUG PY 2016 VL 152 IS 2 BP 264 EP 272 DI 10.1093/toxsci/kfw090 PG 9 WC Toxicology SC Toxicology GA DY7HN UT WOS:000385300400004 PM 27462126 ER PT J AU Judson, R Houck, K Martin, M Richard, AM Knudsen, TB Shah, I Little, S Wambaugh, J Setzer, RW Kothya, P Phuong, J Filer, D Smith, D Reif, D Rotroff, D Kleinstreuer, N Sipes, N Xia, MH Huang, RL Crofton, K Thomas, RS AF Judson, Richard Houck, Keith Martin, Matt Richard, Ann M. Knudsen, Thomas B. Shah, Imran Little, Stephen Wambaugh, John Setzer, R. Woodrow Kothya, Parth Phuong, Jimmy Filer, Dayne Smith, Doris Reif, David Rotroff, Daniel Kleinstreuer, Nicole Sipes, Nisha Xia, Menghang Huang, Ruili Crofton, Kevin Thomas, Russell S. TI Analysis of the Effects of Cell Stress and Cytotoxicity on In Vitro Assay Activity Across a Diverse Chemical and Assay Space SO TOXICOLOGICAL SCIENCES LA English DT Article DE In vitro; high-throughput screening; oxidative stress; cytotoxicity; cell stress ID EPAS TOXCAST PROGRAM; ENVIRONMENTAL CHEMICALS; RISK-ASSESSMENT; BISPHENOL-A; BIOLOGY; MECHANISMS; FRAMEWORK; RECEPTOR; TOXICOLOGY; RELEVANCE AB Chemical toxicity can arise from disruption of specific biomolecular functions or through more generalized cell stress and cytotoxicity-mediated processes. Here, responses of 1060 chemicals including pharmaceuticals, natural products, pesticidals, consumer, and industrial chemicals across a battery of 815 in vitro assay endpoints from 7 high-throughput assay technology platforms were analyzed in order to distinguish between these types of activities. Both cell-based and cell-free assays showed a rapid increase in the frequency of responses at concentrations where cell stress/cytotoxicity responses were observed in cell-based assays. Chemicals that were positive on at least 2 viability/cytotoxicity assays within the concentration range tested (typically up to 100 mu M) activated a median of 12% of assay endpoints whereas those that were not cytotoxic in this concentration range activated 1.3% of the assays endpoints. The results suggest that activity can be broadly divided into: (1) specific biomolecular interactions against one or more targets (eg, receptors or enzymes) at concentrations below which overt cytotoxicity-associated activity is observed; and (2) activity associated with cell stress or cytotoxicity, which may result from triggering specific cell stress pathways, chemical reactivity, physico-chemical disruption of proteins or membranes, or broad low-affinity non-covalent interactions. Chemicals showing a greater number of specific biomolecular interactions are generally designed to be bioactive (pharmaceuticals or pesticidal active ingredients), whereas intentional food-use chemicals tended to show the fewest specific interactions. The analyses presented here provide context for use of these data in ongoing studies to predict in vivo toxicity from chemicals lacking extensive hazard assessment. C1 [Judson, Richard; Houck, Keith; Martin, Matt; Richard, Ann M.; Knudsen, Thomas B.; Shah, Imran; Little, Stephen; Wambaugh, John; Setzer, R. Woodrow; Kothya, Parth; Phuong, Jimmy; Filer, Dayne; Smith, Doris; Crofton, Kevin; Thomas, Russell S.] US EPA, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. [Reif, David; Rotroff, Daniel] North Carolina State Univ, Dept Stat, Raleigh, NC USA. [Kleinstreuer, Nicole] ILS NICEATM, Res Triangle Pk, NC USA. [Sipes, Nisha] Natl Toxicol Program, Res Triangle Pk, NC USA. [Xia, Menghang; Huang, Ruili] NIH Natl Ctr Adv Translat Sci, Rockville, MD USA. RP Judson, R (reprint author), US EPA, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. EM Judson.richard@epa.gov OI Kleinstreuer, Nicole/0000-0002-7914-3682 FU U.S. EPA FX All funding provided by the U.S. EPA. NR 54 TC 2 Z9 2 U1 10 U2 10 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD AUG PY 2016 VL 152 IS 2 BP 323 EP 339 DI 10.1093/toxsci/kfw092 PG 17 WC Toxicology SC Toxicology GA DY7HN UT WOS:000385300400009 PM 27208079 ER PT J AU Detenbeck, NE Morrison, AC Abele, RW Kopp, DA AF Detenbeck, Naomi E. Morrison, Alisa C. Abele, Ralph W. Kopp, Darin A. TI Spatial statistical network models for stream and river temperature in New England, USA SO WATER RESOURCES RESEARCH LA English DT Article DE stream; river; temperature regime; spatial statistical model; New England ID LAND-USE CHANGE; WATER TEMPERATURE; CLIMATE-CHANGE; THERMAL HETEROGENEITY; NEURAL-NETWORK; UNITED-STATES; HABITAT; SURFACES; PATTERNS; REGIMES AB Watershed managers are challenged by the need for predictive temperature models with sufficient accuracy and geographic breadth for practical use. We described thermal regimes of New England rivers and streams based on a reduced set of metrics for the May-September growing season (July or August median temperature, diurnal rate of change, and magnitude and timing of growing season maximum) chosen through principal component analysis of 78 candidate metrics. We then developed and assessed spatial statistical models for each of these metrics, incorporating spatial autocorrelation based on both distance along the flow network and Euclidean distance between points. Calculation of spatial autocorrelation based on travel or retention time in place of network distance yielded tighter-fitting Torgegrams with less scatter but did not improve overall model prediction accuracy. We predicted monthly median July or August stream temperatures as a function of median air temperature, estimated urban heat island effect, shaded solar radiation, main channel slope, watershed storage (percent lake and wetland area), percent coarse-grained surficial deposits, and presence or maximum depth of a lake immediately upstream, with an overall root-mean-square prediction error of 1.4 and 1.5 degrees C, respectively. Growing season maximum water temperature varied as a function of air temperature, local channel slope, shaded August solar radiation, imperviousness, and watershed storage. Predictive models for July or August daily range, maximum daily rate of change, and timing of growing season maximum were statistically significant but explained a much lower proportion of variance than the above models (5-14% of total). C1 [Detenbeck, Naomi E.; Morrison, Alisa C.; Kopp, Darin A.] US EPA, Atlantic Ecol Div, Narragansett, RI 02882 USA. [Abele, Ralph W.] US EPA, Reg 1, Boston, MA USA. [Kopp, Darin A.] Arizona State Univ, Wrigley Inst, Tempe, AZ USA. RP Detenbeck, NE (reprint author), US EPA, Atlantic Ecol Div, Narragansett, RI 02882 USA. EM detenbeck.naomi@epa.gov FU U.S. Environmental Protection Agency through EPA Region 1 RARE project; US EPA Green Infrastructure research program; U.S. Environmental Protection Agency FX A. C. M. is a former student services contractor and a current ORISE participant at the U.S. Environmental Protection Agency. D. A. K. is a former student services contractor at AED. Data associated with figures in this manuscript will be available at: https://edg.epa.gov/metadata/catalog/main/home.page within a few months of publication. Upon publication, supporting geospatial data (stream network, observed, and predicted water temperature metrics, predictor variables) will be made available through EPA's EDM application (www.epa.gov/edm). This is contribution number ORD-013257 of the Atlantic Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency. Support for this work was provided by the U.S. Environmental Protection Agency through EPA Region 1 RARE project funding for a student services contract and through the US EPA Green Infrastructure research program. The information in this document has been funded wholly by the U.S. Environmental Protection Agency. It has been subjected to Agency review and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. The authors wish to sincerely thank the many individuals in state and Federal agencies and nongovernmental organizations who collected the stream temperature data represented in this publication and made it available for analysis (Table 1). We also thank Paul Seelbach, Anne Kuhn, and Jeff Hollister for providing preliminary technical reviews of this manuscript and three anonymous reviewers of the final publication. NR 84 TC 1 Z9 1 U1 7 U2 7 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD AUG PY 2016 VL 52 IS 8 BP 6018 EP 6040 DI 10.1002/2015WR018349 PG 23 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DW5KT UT WOS:000383684400018 ER PT J AU Price, AM Pospelova, V Coffin, MRS Latimer, JS Chmura, GL AF Price, Andrea M. Pospelova, Vera Coffin, Michael R. S. Latimer, James S. Chmura, Gail L. TI Biogeography of dinoflagellate cysts in northwest Atlantic estuaries SO ECOLOGY AND EVOLUTION LA English DT Article DE Coastal waters; estuary type; northeast USA; palynology; phytoplankton; Prince Edward Island; water quality ID NORTHEASTERN UNITED-STATES; WEST-COAST; ENVIRONMENTAL-FACTORS; PYRODINIUM-BAHAMENSE; SPATIAL-DISTRIBUTION; NORTHERN-HEMISPHERE; SURFACE SEDIMENTS; MARINE-SEDIMENTS; NARRAGANSETT BAY; PHYTOPLANKTON AB Few biogeographic studies of dinoflagellate cysts include the near-shore estuarine environment. We determine the effect of estuary type, biogeography, and water quality on the spatial distribution of organic-walled dinoflagellate cysts from the Northeast USA (Maine to Delaware) and Canada (Prince Edward Island). A total of 69 surface sediment samples were collected from 27 estuaries, from sites with surface salinities > 20. Dinoflagellate cysts were examined microscopically and compared to environmental parameters using multivariate ordination techniques. The spatial distribution of cyst taxa reflects biogeographic provinces established by other marine organisms, with Cape Cod separating the northern Acadian Province from the southern Virginian Province. Species such as Lingulodinium machaerophorum and Polysphaeridinium zoharyi were found almost exclusively in the Virginian Province, while others such as Dubridinium spp. and Islandinium? cezare were more abundant in the Acadian Province. Tidal range, sea surface temperature (SST), and sea surface salinity (SSS) are statistically significant parameters influencing cyst assemblages. Samples from the same type of estuary cluster together in canonical correspondence analysis when the estuaries are within the same biogeographic province. The large geographic extent of this study, encompassing four main estuary types (riverine, lagoon, coastal embayment, and fjord), allowed us to determine that the type of estuary has an important influence on cyst assemblages. Due to greater seasonal variations in SSTs and SSSs in estuaries compared to the open ocean, cyst assemblages show distinct latitudinal trends. The estuarine context is important for understanding present-day species distribution, the factors controlling them, and to better predict how they may change in the future. C1 [Price, Andrea M.; Chmura, Gail L.] McGill Univ, Dept Geog, 805 Sherbrooke St West, Montreal, PQ H3A 0B9, Canada. [Pospelova, Vera] Univ Victoria, Sch Earth & Ocean Sci, OEASB A405,POB 1700 STN CSC, Victoria, BC V8W 2Y2, Canada. [Coffin, Michael R. S.] Univ Prince Edward Isl, Dept Biol, Charlottetown, PE C1A 4P3, Canada. [Latimer, James S.] US EPA, Off Res & Dev, Narragansett, RI 02882 USA. RP Price, AM (reprint author), McGill Univ, Dept Geog, 805 Sherbrooke St West, Montreal, PQ H3A 0B9, Canada. EM pricea@uvic.ca FU Natural Sciences and Engineering Research Council of Canada FX Natural Sciences and Engineering Research Council of Canada NR 77 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD AUG PY 2016 VL 6 IS 16 BP 5648 EP 5662 DI 10.1002/ece3.2262 PG 15 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DT6FF UT WOS:000381578400008 PM 27547344 ER PT J AU Chen, HL AF Chen, Honglei TI Are We Ready for a Potential Increase in Parkinson Incidence? SO JAMA NEUROLOGY LA English DT Editorial Material ID DISEASE; SMOKING; RISK C1 [Chen, Honglei] Natl Inst Environm Hlth Sci, Epidemiol Branch, 111 TW Alexander Dr,POB 12233,Mail Drop A3-05, Res Triangle Pk, NC 27709 USA. RP Chen, HL (reprint author), Natl Inst Environm Hlth Sci, Epidemiol Branch, 111 TW Alexander Dr,POB 12233,Mail Drop A3-05, Res Triangle Pk, NC 27709 USA. EM chenh2@niehs.nih.gov OI Chen, Honglei/0000-0003-3446-7779 NR 12 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 2168-6149 EI 2168-6157 J9 JAMA NEUROL JI JAMA Neurol. PD AUG PY 2016 VL 73 IS 8 BP 919 EP 921 DI 10.1001/jamaneurol.2016.1599 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA DX2MS UT WOS:000384204900009 PM 27322659 ER PT J AU Carbone, M Kanodia, S Chao, A Miller, A Wali, A Weissman, D Adjei, A Baumann, F Boffetta, P Buck, B de Perrot, M Dogan, AU Gavett, S Gualtieri, A Hassan, R Hesdorffer, M Hirsch, FR Larson, D Mao, WM Masten, S Pass, HI Peto, J Pira, E Steele, I Tsao, A Woodard, GA Yang, HN Malik, S AF Carbone, Michele Kanodia, Shreya Chao, Ann Miller, Aubrey Wali, Anil Weissman, David Adjei, Alex Baumann, Francine Boffetta, Paolo Buck, Brenda de Perrot, Marc Dogan, A. Umran Gavett, Steve Gualtieri, Alessandro Hassan, Raffit Hesdorffer, Mary Hirsch, Fred R. Larson, David Mao, Weimin Masten, Scott Pass, Harvey I. Peto, Julian Pira, Enrico Steele, Ian Tsao, Anne Woodard, Gavitt Alida Yang, Haining Malik, Shakun TI Consensus Report of the 2015 Weinman International Conference on Mesothelioma SO JOURNAL OF THORACIC ONCOLOGY LA English DT Article DE Mesothelioma; BAP1; Asbestos; Erionite; Biomarkers; Genetics; Therapy ID MALIGNANT-PLEURAL-MESOTHELIOMA; GERMLINE BAP1 MUTATIONS; PHASE-II TRIAL; NATURALLY-OCCURRING ASBESTOS; SERUM OSTEOPONTIN LEVELS; EXTRAPLEURAL PNEUMONECTOMY; NEOADJUVANT CHEMOTHERAPY; INDUCTION CHEMOTHERAPY; TRIMODALITY THERAPY; TUMOR-SUPPRESSOR AB On November 9 and 10, 2015, the International Conference on Mesothelioma in Populations Exposed to Naturally Occurring Asbestiform Fibers was held at the University of Hawaii Cancer Center in Honolulu, Hawaii. The meeting was cosponsored by the International Association for the Study of Lung Cancer, and the agenda was designed with significant input from staff at the U.S. National Cancer Institute and National Institute of Environmental Health Sciences. A multidisciplinary group of participants presented updates reflecting a range of disciplinary perspectives, including mineralogy, geology, epidemiology, toxicology, biochemistry, molecular biology, genetics, public health, and clinical oncology. The group identified knowledge gaps that are barriers to preventing and treating malignant mesothelioma (MM) and the required next steps to address barriers. This manuscript reports the group's efforts and focus on strategies to limit risk to the population and reduce the incidence of MM. Four main topics were explored: genetic risk, environmental exposure, biomarkers, and clinical interventions. Genetics plays a critical role in MM when the disease occurs in carriers of germline BRCA1 associated protein 1 mutations. Moreover, it appears likely that, in addition to BRCA1 associated protein 1, other yet unknown genetic variants may also influence the individual risk for development of MM, especially after exposure to asbestos and related mineral fibers. MM is an almost entirely preventable malignancy as it is most often caused by exposure to commercial asbestos or mineral fibers with asbestos-like health effects, such as erionite. In the past in North America and in Europe, the most prominent source of exposure was related to occupation. Present regulations have reduced occupational exposure in these countries; however, some people continue to be exposed to previously installed asbestos in older construction and other settings. Moreover, an increasing number of people are being exposed in rural areas that contain noncommercial asbestos, erionite, and other mineral fibers in soil or rock (termed naturally occurring asbestos [NOA]) and are being developed. Public health authorities, scientists, residents, and other affected groups must work together in the areas where exposure to asbestos, including NOA, has been documented in the environment to mitigate or reduce this exposure. Although a blood biomarker validated to be effective for use in screening and identifying MM at an early stage in asbestos/NOA-exposed populations is not currently available, novel biomarkers presented at the meeting, such as high mobility group box 1 and fibulin-3, are promising. There was general agreement that current treatment for MM, which is based on surgery and standard chemotherapy, has a modest effect on the overall survival (OS), which remains dismal. Additionally, although much needed novel therapeutic approaches for MM are being developed and explored in clinical trials, there is a critical need to invest in prevention research, in which there is a great opportunity to reduce the incidence and mortality from MM. 2016 International Association for the Study of Lung Cancer. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Carbone, Michele; Kanodia, Shreya; Larson, David; Yang, Haining] Univ Hawaii, Thorac Oncol, Ctr Canc, Honolulu, HI 96822 USA. [Kanodia, Shreya] Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Los Angeles, CA 90048 USA. [Kanodia, Shreya] Cedars Sinai Med Ctr, Dept Biomed Sci, Los Angeles, CA 90048 USA. [Chao, Ann] NCI, Ctr Global Hlth, NIH, Bethesda, MD 20892 USA. [Miller, Aubrey] NIEHS, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Wali, Anil] NCI, Ctr Reduce Canc Hlth Dispar, NIH, Bethesda, MD 20892 USA. [Weissman, David] NIOSH, Resp Hlth Div, Ctr Dis Control & Prevent, Morgantown, WV USA. [Adjei, Alex] Mayo Clin, Rochester, MN USA. [Baumann, Francine] Univ New Caledonia, ERIM, Noumea, New Caledonia. [Boffetta, Paolo] Icahn Sch Med Mt Sinai, Tisch Canc Inst, New York, NY USA. [Buck, Brenda] Univ Nevada, Dept Geosci, Las Vegas, NV 89154 USA. [de Perrot, Marc] Univ Hlth Network, Princess Margaret Canc Ctr, Toronto, ON, Canada. [Dogan, A. Umran] Univ Iowa, Chem & Biochem Engn Dept, Iowa City, IA USA. [Dogan, A. Umran] Univ Iowa, Ctr Global & Reg Environm Res, Iowa City, IA USA. [Gavett, Steve] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Gualtieri, Alessandro] Univ Modena, Chem Earth Sci Dept, Modena, Italy. [Hassan, Raffit] NIH, Thorac Oncol Branch, Ctr Canc Res, Bldg 10, Bethesda, MD 20892 USA. [Hesdorffer, Mary] Mesothelioma Appl Res Fdn, Alexandria, VA USA. [Hirsch, Fred R.] Univ Colorado, Ctr Canc, Denver, CO 80202 USA. [Mao, Weimin] Zhejiang Canc Hosp, Canc Res Inst, Hangzhou, Zhejiang, Peoples R China. [Mao, Weimin] Key Lab Diag & Treatment Technol Thorac Oncol Zhe, Hangzhou, Zhejiang, Peoples R China. [Masten, Scott] NIEHS, Natl Toxicol Program, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Pass, Harvey I.] NYU, Langone Med Ctr, Cardiothorac Surg, New York, NY USA. [Peto, Julian] London Sch Hyg & Trop Med, Canc Res UK, London, England. [Pira, Enrico] Univ Turin, Dept Publ Hlth & Pediat, Turin, Italy. [Steele, Ian] Notre Dame Univ, Notre Dame Integrated Imaging Facil, Notre Dame, IN USA. [Tsao, Anne] Univ Texas MD Anderson Canc Ctr, Dept Thorac & Head & Neck Med Oncol, Div Canc Med, Houston, TX 77030 USA. [Woodard, Gavitt Alida] Univ Calif San Francisco, Thorac Surg, San Francisco, CA 94143 USA. [Malik, Shakun] NCI, Canc Therapy Evaluat Program, NIH, Bethesda, MD 20892 USA. RP Carbone, M (reprint author), Univ Hawaii, Ctr Canc, 701 Ilalo St,Room 437, Honolulu, HI 96813 USA. EM mcarbone@cc.hawaii.edu RI masten, scott/R-1403-2016 OI masten, scott/0000-0002-7847-181X FU Jack Mishkin Discovery Fund for Mesothelioma Research from the Tower Cancer Research Foundation; Bureau of Land Management of the U.S. Department of the Interior [L13AC00237] FX The meeting was made possible by a generous donation from the Barry and Virginia Weinman Foundation and the International Association for the Study of Lung Cancer. Dr. Kanodia is funded by the Jack Mishkin Discovery Fund for Mesothelioma Research from the Tower Cancer Research Foundation. Dr. Buck has a grant funded by the Bureau of Land Management of the U.S. Department of the Interior to study NOA in Nevada (no. L13AC00237). The findings and conclusions in this report are those of the authors and do not necessarily represent the views of any part of the U.S. Government. NR 140 TC 7 Z9 7 U1 14 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1556-0864 EI 1556-1380 J9 J THORAC ONCOL JI J. Thorac. Oncol. PD AUG PY 2016 VL 11 IS 8 BP 1246 EP 1262 DI 10.1016/j.jtho.2016.04.028 PG 17 WC Oncology; Respiratory System SC Oncology; Respiratory System GA DS8XW UT WOS:000381067300008 PM 27453164 ER PT J AU Shatkin, JA Ong, KJ Beaudrie, C Clippinger, AJ Hendren, CO Haber, LT Hill, M Holden, P Kennedy, AJ Kim, B MacDonell, M Powers, CM Sharma, M Sheremeta, L Stone, V Sultan, Y Turley, A White, RH AF Shatkin, J. A. Ong, Kimberly J. Beaudrie, Christian Clippinger, Amy J. Hendren, Christine Ogilvie Haber, Lynne T. Hill, Myriam Holden, Patricia Kennedy, Alan J. Kim, Baram MacDonell, Margaret Powers, Christina M. Sharma, Monita Sheremeta, Lorraine Stone, Vicki Sultan, Yasir Turley, Audrey White, Ronald H. TI Advancing Risk Analysis for Nanoscale Materials: Report from an International Workshop on the Role of Alternative Testing Strategies for Advancement SO RISK ANALYSIS LA English DT Article DE Alternative testing strategies; expert workshop; nanomaterials; nanotoxicology; risk analysis ID ENGINEERED NANOMATERIALS; IN-VITRO; NANOPARTICLES; NANOTOXICOLOGY; TOXICITY; IDENTIFICATION; CYTOTOXICITY; ENVIRONMENT; PARTICLES; DOSIMETRY AB The Society for Risk Analysis (SRA) has a history of bringing thought leadership to topics of emerging risk. In September 2014, the SRA Emerging Nanoscale Materials Specialty Group convened an international workshop to examine the use of alternative testing strategies (ATS) for manufactured nanomaterials (NM) from a risk analysis perspective. Experts in NM environmental health and safety, human health, ecotoxicology, regulatory compliance, risk analysis, and ATS evaluated and discussed the state of the science for in vitro and other alternatives to traditional toxicology testing for NM. Based on this review, experts recommended immediate and near-term actions that would advance ATS use in NM risk assessment. Three focal areashuman health, ecological health, and exposure considerationsshaped deliberations about information needs, priorities, and the next steps required to increase confidence in and use of ATS in NM risk assessment. The deliberations revealed that ATS are now being used for screening, and that, in the near term, ATS could be developed for use in read-across or categorization decision making within certain regulatory frameworks. Participants recognized that leadership is required from within the scientific community to address basic challenges, including standardizing materials, protocols, techniques and reporting, and designing experiments relevant to real-world conditions, as well as coordination and sharing of large-scale collaborations and data. Experts agreed that it will be critical to include experimental parameters that can support the development of adverse outcome pathways. Numerous other insightful ideas for investment in ATS emerged throughout the discussions and are further highlighted in this article. C1 [Shatkin, J. A.; Ong, Kimberly J.] Vireo Advisors LLC, POB 51368, Boston, MA 02130 USA. [Beaudrie, Christian] Compass RM, Vancouver, CA USA. [Clippinger, Amy J.; Sharma, Monita] PETA Int Sci Consortium Ltd, London, England. [Hendren, Christine Ogilvie] Duke Univ, Ctr Environm Implicat NanoTechnol, Durham, NC USA. [Haber, Lynne T.] TERA, Cincinnati, OH USA. [Hill, Myriam] Hlth Canada, Ottawa, ON, Canada. [Holden, Patricia] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, ERI, Santa Barbara, CA 93106 USA. [Holden, Patricia] Univ Calif Santa Barbara, UC CEIN, Santa Barbara, CA 93106 USA. [Kennedy, Alan J.] US Army Engn Res & Dev Ctr, Environm Lab, Vicksburg, MS USA. [MacDonell, Margaret] Argonne Natl Lab, Div Environm Sci, 9700 S Cass Ave, Argonne, IL 60439 USA. [Powers, Christina M.] US EPA, Off Air & Radiat, Off Transportat & Air Qual, Ann Arbor, MI USA. [Sheremeta, Lorraine] Alberta Ingenu Labs, Edmonton, AB, Canada. [Stone, Vicki] Heriot Watt Univ, John Muir Bldg Gait 1, Edinburgh, Midlothian, Scotland. [Sultan, Yasir] Environm Canada, Gatineau, PQ, Canada. [Turley, Audrey] ICF Int, Durham, NC USA. [White, Ronald H.] RH White Consultants, Silver Spring, MD USA. RP Shatkin, JA (reprint author), Vireo Advisors LLC, POB 51368, Boston, MA 02130 USA. EM jashatkin@gmail.com FU National Science Foundation; Society for Risk Analysis Emerging Nanoscale Materials Specialty Group; Alberta Ingenuity Labs; PETA International Science Consortium Ltd.; Bergeson Campbell PC; American Chemistry Council; Keller and Heckman LLP; Toxicology Excellence in Risk Assessment FX The views expressed in this article are those of the authors and do not necessarily represent the views or policies or positions of their respective organizations. The authors wish to acknowledge the generous support of the following workshop co-sponsors: the National Science Foundation; the Society for Risk Analysis Emerging Nanoscale Materials Specialty Group; Alberta Ingenuity Labs; PETA International Science Consortium Ltd.; Bergeson & Campbell PC; American Chemistry Council; Keller and Heckman LLP; Toxicology Excellence in Risk Assessment; and in-kind contributions from: American Chemical Society, Center for the Environmental Implications of Nanotechnology; George Washington University Milken Institute for Public Health, Society for Toxicology Nanotoxicology Specialty Section; Society for Toxicology and Chemistry Nanotechnology Advisory Group; Sustainable Nanotechnology Organization; UC Center for the Environmental Implications of Nanotechnology; and U.S. Army Engineer Research and Development Center. The authors further wish to acknowledge the cooperation of the Organisation for Economic Cooperation and Development Working Party on Manufactured Nanomaterials. NR 60 TC 2 Z9 2 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD AUG PY 2016 VL 36 IS 8 SI SI BP 1520 EP 1537 DI 10.1111/risa.12683 PG 18 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA DW6OU UT WOS:000383771600004 PM 27510619 ER PT J AU Du, X Crawford, DL Nacci, DE Oleksiak, MF AF Du, Xiao Crawford, Douglas L. Nacci, Diane E. Oleksiak, Marjorie F. TI Heritable oxidative phosphorylation differences in a pollutant resistant Fundulus heteroclitus population SO AQUATIC TOXICOLOGY LA English DT Article DE Fundulus heteroclitus; Oxidative phosphorylation; F3 generation; Benzo [a] pyrene; Elizabeth river ID POLYCYCLIC AROMATIC-HYDROCARBONS; ELIZABETH RIVER; MITOCHONDRIAL DYSFUNCTION; NATURAL-POPULATIONS; GENE-EXPRESSION; EXPOSURE; KILLIFISH; ESTUARY; INTRAPERITONEAL; TERATOGENESIS AB Populations can adapt to stress including recent anthropogenic pollution. Our published data suggests heritable differences in hepatocyte oxidative phosphorylation (OxPhos) metabolism in field-caught killifish (Fundulus heteroclitus) from the highly polluted Elizabeth River, VA, USA, relative to fish from a nearby, relatively unpolluted reference site in King's Creek VA. Consistent with other studies showing that Elizabeth River killifish are resistant to some of the toxic effects of certain contaminants, OxPhos measurements in hepatocytes from field-caught King's Creek but not field-caught Elizabeth River killifish were altered by acute benzo [a] pyrene exposures. To more definitively test whether the enhanced OxPhos metabolism and toxicity resistance are heritable, we measured OxPhos metabolism in a laboratory-reared F3 generation from the Elizabeth River population versus a laboratory-reared F1generation from the King's Creek population and compared these results to previous data from the field-caught fish. The F3 Elizabeth River fish compared to Fl King's Creek fish had significantly higher State 3 respiration (routine metabolism) and complex II activity, and significantly lower complex I activity. The consistently higher routine metabolism in the F3 and field-caught Elizabeth River fish versus Fl and field-caught King's Creek fish implies a heritable change in OxPhos function. The observation that LEAK, E-State, Complex I and Complex II were different in laboratory bred versus field-caught fish suggests that different physiological mechanisms produce the enhanced OxPhos differences. Finally, similar to field-caught Elizabeth River fish, acute benzo [a] pyrene exposure did not affect OxPhos function of the laboratory-reared F3 generation, supporting the heritability of the toxicity resistance. Overall, these results suggest that the Elizabeth River population has evolved genetic changes in physiological homeostasis that enhance routine metabolism, and we speculate that these genetic changes interact with environmental factors altering the physiological mechanisms (e.g., alter LEAK, Complex I, and electron transfer system capacity) used to achieve this enhanced metabolism. (C) 2016 Elsevier B.V. All rights reserved. C1 [Du, Xiao; Crawford, Douglas L.; Oleksiak, Marjorie F.] Univ Miami, Rosenstiel Sch Marine & Atmospher Sci, Marine Biol & Ecol, 4600 Rickenbacker Causeway, Miami, FL 33149 USA. [Nacci, Diane E.] US EPA, Populat Ecol Branch, Atlantic Ecol Div, Off Res & Dev, 27 Tarzwell Dr, Narragansett, RI 02882 USA. RP Du, X; Oleksiak, MF (reprint author), Univ Miami, Rosenstiel Sch Marine & Atmospher Sci, Marine Biol & Ecol, 4600 Rickenbacker Causeway, Miami, FL 33149 USA. EM xdu@rsmas.miami.edu; moleksiak@rsmas.miami.edu FU NSF [MCB-1158241]; Provost Award (University of Miami) FX This research was supported by NSF MCB-1158241 and Provost Award (University of Miami) to M.F.O. NR 36 TC 1 Z9 1 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-445X EI 1879-1514 J9 AQUAT TOXICOL JI Aquat. Toxicol. PD AUG PY 2016 VL 177 BP 44 EP 50 DI 10.1016/j.aquatox.2016.05.007 PG 7 WC Marine & Freshwater Biology; Toxicology SC Marine & Freshwater Biology; Toxicology GA DT5NK UT WOS:000381529700006 PM 27239777 ER PT J AU Teague, A Russell, M Harvey, J Dantin, D Nestlerode, J Alvarez, E AF Teague, A. Russell, M. Harvey, J. Dantin, D. Nestlerode, J. Alvarez, E. TI A spatially-explicit technique for evaluation of alternative scenarios in the context of ecosystem goods and services SO ECOSYSTEM SERVICES LA English DT Article DE Ecosystem goods and services; Scenario assessment; Tampa bay; Conservation ID ORGANIC-MATTER MINERALIZATION; UNITED-STATES; FRESH-WATER; CARBON SEQUESTRATION; PHYTOPLANKTON BIOMASS; DENITRIFICATION RATES; MANGROVE SEDIMENTS; INORGANIC NITROGEN; FORESTED WETLANDS; DECISION-MAKING AB Ecosystems contribute to maintaining human well-being directly through provision of goods and indirectly through provision of services that support clean water, clean air, flood protection and atmospheric stability. Transparently accounting for biophysical attributes from which humans derive benefit is essential to support dialog among the public, resource managers, decision makers, and scientists. We analyzed the potential ecosystem goods and services production from alternative future land use scenarios in the US Tampa Bay region. Ecosystem goods and service metrics included carbon sequestration, nitrogen removal, air pollutant removal, and stormwater retention. Each scenario was compared to a 2006 baseline land use. Estimated production of denitrification services changed by 28% and carbon sequestration by 20% between 2006 and the "business as usual" scenario. An alternative scenario focused on "natural resource protection" resulted in an estimated 9% loss in air pollution removal. Stormwater retention was estimated to change 18% from 2006 to 2060 projections. Cost effective areas for conservation, almost 1588 ha, beyond current conservation lands, were identified by comparing ecosystem goods and services production to assessed land values. Our ecosystem goods and services approach provides a simple and quantitative way to examine a more complete set of potential outcomes from land use decisions. (C) 2016 Elsevier B.V. All rights reserved. C1 [Teague, A.; Russell, M.; Harvey, J.; Dantin, D.; Nestlerode, J.; Alvarez, E.] US EPA, Gulf Ecol Div, NHEERL, ORD, 1 Sabine Dr, Gulf Breeze, FL 32561 USA. RP Teague, A (reprint author), US EPA, Gulf Ecol Div, NHEERL, ORD, 1 Sabine Dr, Gulf Breeze, FL 32561 USA. EM ateague@sara-tx.org NR 99 TC 0 Z9 0 U1 23 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2212-0416 J9 ECOSYST SERV JI Ecosyst. Serv. PD AUG PY 2016 VL 20 BP 15 EP 29 DI 10.1016/j.ecoser.2016.06.001 PG 15 WC Ecology; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA DU5QX UT WOS:000382267500002 ER PT J AU Jiang, Y Yuan, Y Neale, A Jackson, L Mehaffey, M AF Jiang, Yan Yuan, Yongping Neale, Anne Jackson, Laura Mehaffey, Megan TI Association between Natural Resources for Outdoor Activities and Physical Inactivity: Results from the Contiguous United States SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Article DE protected areas; community health; physical inactivity; spatial autocorrelation; spatial lag model ID GREEN SPACE; HEALTH; ENVIRONMENTS; WORLDWIDE; CHILDREN; BENEFITS; OBESITY; LIFE AB Protected areas including national/state parks and recreational waters are excellent natural resources that promote physical activity and interaction with Nature, which can relieve stress and reduce disease risk. Despite their importance, however, their contribution to human health has not been properly quantified. This paper seeks to evaluate quantitatively how national/state parks and recreational waters are associated with human health and well-being, taking into account of the spatial dependence of environmental variables for the contiguous U.S., at the county level. First, we describe available natural resources for outdoor activities (ANROA), using national databases that include features from the Protected Areas Database, NAVSTREETS, and ATTAINSGEO 305(b) Waters. We then use spatial regression techniques to explore the association of ANROA and socioeconomic status factors on physical inactivity rates. Finally, we use variance analysis to analyze ANROA's influence on income-related health inequality. We found a significantly negative association between ANROA and the rate of physical inactivity: ANROA and the spatial effect explained 69%, nationwide, of the variation in physical inactivity. Physical inactivity rate showed a strong spatial dependence-influenced not only by its own in-county ANROA, but also by that of its neighbors ANROA. Furthermore, community groups at the same income level and with the highest ANROA, always had the lowest physical inactivity rate. This finding may help to guide future land use planning and community development that will benefit human health and well-being. C1 [Jiang, Yan; Yuan, Yongping; Neale, Anne; Jackson, Laura; Mehaffey, Megan] US EPA, Off Res & Dev, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. RP Yuan, Y (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM enaj1125@gmail.com; yuan.yongping@epa.gov; neale.anne@epa.gov; jackson.laura@epa.gov; mehaffey.megan@epa.gov OI Jiang, Yan/0000-0002-7380-4478 NR 44 TC 0 Z9 0 U1 10 U2 10 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD AUG PY 2016 VL 13 IS 8 AR 830 DI 10.3390/ijerph13080830 PG 12 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DU8KV UT WOS:000382462900085 ER PT J AU Brooks, AL Hoel, DG Preston, RJ AF Brooks, Antone L. Hoel, David G. Preston, R. Julian TI The role of dose rate in radiation cancer risk: evaluating the effect of dose rate at the molecular, cellular and tissue levels using key events in critical pathways following exposure to low LET radiation SO INTERNATIONAL JOURNAL OF RADIATION BIOLOGY LA English DT Review DE Cellular radiobiology; gene expression; low dose rate; radiation; radionuclides ID INDUCED NEOPLASTIC TRANSFORMATION; ATOMIC-BOMB SURVIVORS; METAPHASE CHROMOSOME-ABERRATIONS; INDUCED GENOMIC INSTABILITY; GENE-EXPRESSION PROFILES; NORMAL HUMAN FIBROBLASTS; BODY GAMMA-IRRADIATION; DOUBLE-STRAND BREAKS; NORMAL HUMAN-CELLS; LOW-LET RADIATION AB Purpose: This review evaluates the role of dose rate on cell and molecular responses. It focuses on the influence of dose rate on key events in critical pathways in the development of cancer. This approach is similar to that used by the U.S. EPA and others to evaluate risk from chemicals. It provides a mechanistic method to account for the influence of the dose rate from low-LET radiation, especially in the low-dose region on cancer risk assessment. Molecular, cellular, and tissues changes are observed in many key events and change as a function of dose rate. The magnitude and direction of change can be used to help establish an appropriate dose rate effectiveness factor (DREF).Conclusions: Extensive data on key events suggest that exposure to low dose-rates are less effective in producing changes than high dose rates. Most of these data at the molecular and cellular level support a large (2-30) DREF. In addition, some evidence suggests that doses delivered at a low dose rate decrease damage to levels below that observed in the controls. However, there are some data human and mechanistic data that support a dose-rate effectiveness factor of 1. In summary, a review of the available molecular, cellular and tissue data indicates that not only is dose rate an important variable in understanding radiation risk but it also supports the selection of a DREF greater than one as currently recommended by ICRP (2007) and BEIR VII (NRC/NAS 2006). C1 [Brooks, Antone L.] Washington State Univ, Environm Sci, Richland, WA USA. [Hoel, David G.] Med Univ South Carolina, Epidemiol, Charleston, SC USA. [Preston, R. Julian] US EPA, NHEERL, MD B105-01, Res Triangle Pk, NC USA. RP Brooks, AL (reprint author), A L877897, Kennewick, WA USA. EM tbrooks@tricity.wsu.edu FU Electric Power Research Institute (EPRI) [10000432, 1-003171-02-01] FX The authors would like to acknowledge the editorial help of Careina C. Brooks and Lezlie A. Couch and the scientific input from Dr Donald Cool and Phung Than. This work was supported by the Electric Power Research Institute (EPRI Agreement No. 10000432 Charge number 1-003171-02-01). NR 165 TC 4 Z9 4 U1 6 U2 8 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0955-3002 EI 1362-3095 J9 INT J RADIAT BIOL JI Int. J. Radiat. Biol. PD AUG PY 2016 VL 92 IS 8 BP 405 EP 426 DI 10.1080/09553002.2016.1186301 PG 22 WC Biology; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Life Sciences & Biomedicine - Other Topics; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA DT5HU UT WOS:000381514100001 PM 27266588 ER PT J AU Barkdoll, BD Kantor, CM Wesseldyke, ES Ghimire, SR AF Barkdoll, Brian D. Kantor, Candice M. Wesseldyke, Eric S. Ghimire, Santosh R. TI Stormwater Low-Impact Development: A Call to Arms for Hydraulic Engineers SO JOURNAL OF HYDRAULIC ENGINEERING LA English DT Article ID POROUS PAVEMENT; SEDIMENT TRANSPORT; MANAGEMENT-PRACTICES; QUALITY PERFORMANCE; RESERVOIR STRUCTURE; SCALE BIORETENTION; NORTH-CAROLINA; AQUATIC PLANT; WATER-QUALITY; SHEAR-STRESS AB Forum papers are thought-provoking opinion pieces or essays founded in fact, sometimes containing speculation, on a civil engineering topic of general interest and relevance to the readership of the journal. The views expressed in this Forum article do not necessarily reflect the views of ASCE or the Editorial Board of the journal. C1 [Barkdoll, Brian D.] Michigan Technol Univ, Dept Civil & Environm Engn, Houghton, MI 49931 USA. [Kantor, Candice M.] Barr Engn, 4300 MarketPointe Dr, Minneapolis, MN 55435 USA. [Wesseldyke, Eric S.] Leggete Brashears & Graham Inc, 511 Valley Ave, Burlington, ND 58722 USA. [Ghimire, Santosh R.] US EPA, 960 Coll Stn Rd, Athens, GA 30605 USA. RP Barkdoll, BD (reprint author), Michigan Technol Univ, Dept Civil & Environm Engn, Houghton, MI 49931 USA. EM barkdoll@mtu.edu; CKantor@barr.com; eric.wesseldyke@lbgnd.com; ghimire.santosh@epa.gov NR 72 TC 0 Z9 0 U1 17 U2 17 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9429 EI 1943-7900 J9 J HYDRAUL ENG JI J. Hydraul. Eng.-ASCE PD AUG PY 2016 VL 142 IS 8 AR 02516002 DI 10.1061/(ASCE)HY.1943-7900.0001152 PG 6 WC Engineering, Civil; Engineering, Mechanical; Water Resources SC Engineering; Water Resources GA DS8TH UT WOS:000381055400007 ER PT J AU Tollanes, MC Wilcox, AJ Stoltenberg, C Lie, RT Moster, D AF Tollanes, Mette C. Wilcox, Allen J. Stoltenberg, Camilla Lie, Rolv T. Moster, Dag TI Neurodevelopmental Disorders or Early Death in Siblings of Children With Cerebral Palsy SO PEDIATRICS LA English DT Article ID PRETERM BIRTH; PREVALENCE; RISK; TERM; SPECTRUM; NORWAY; COHORT AB OBJECTIVES: To explore the presence of shared underlying causes of cerebral palsy (CP) and other neurodevelopmental disorders, by examining risks of other disorders in siblings of children with CP. METHODS: We used Norwegian national registries to identify 1.4 million pairs of full siblings (singletons) and 28 000 sets of twins born from 1967 to 2006, identify stillbirths and neonatal deaths, and find individuals with CP, epilepsy, intellectual disability, autism spectrum disorders, attention-deficit/hyperactivity disorder, blindness, deafness, schizophrenia, and bipolar disorder. Associations between CP in 1 sibling and neurodevelopmental disorders or early death in other siblings were estimated using logistic regression models. RESULTS: There were 5707 neonatal survivors (beyond 28 days) with CP (2.5/1000). These children had substantial comorbidity (eg, 29% had epilepsy). Singleton siblings of (singleton) children with CP had increased risks of neurodevelopmental problems, including epilepsy (odds ratio [OR], 1.8 [95% confidence interval (CI), 1.5-2.5]), intellectual disability (OR, 2.3 [95% CI, 1.8-2.9]), autism spectrum disorders (OR, 1.6 [95% CI, 1.1-2.2]), attention-deficit/hyperactivity disorder (OR 1.3 [95% CI, 1.1-1.6]), blindness (OR 2.4 [95% CI, 1.1-5.4]), and schizophrenia (OR 2.0 [95% CI, 1.2-3.2]). There was no increase in risk of bipolar disorder (OR 1.0 [95% CI, 0.6-1.6]). Families with children with CP also had increased risk of losing another child in the perinatal period (stillbirth OR, 1.8 [95% CI, 1.5-2.3]; neonatal death OR, 1.7 [95% CI, 1.3-2.2]). Associations were stronger within sets of twins. CONCLUSIONS: Siblings of a child with CP were at increased risk for a variety of other neurodevelopmental morbidities, as well as early death, indicating the presence of shared underlying causes. C1 [Tollanes, Mette C.; Stoltenberg, Camilla; Lie, Rolv T.; Moster, Dag] Univ Bergen, Dept Global Publ Hlth & Primary Care, Postboks 7804, N-5018 Bergen, Norway. [Tollanes, Mette C.; Stoltenberg, Camilla; Lie, Rolv T.; Moster, Dag] Norwegian Inst Publ Hlth, Oslo, Norway. [Wilcox, Allen J.] Natl Inst Environm Hlth Sci, Durham, NC USA. [Moster, Dag] Haukeland Hosp, Bergen, Norway. RP Tollanes, MC (reprint author), Univ Bergen, Dept Global Publ Hlth & Primary Care, Postboks 7804, N-5018 Bergen, Norway. EM mette.tollanes@uib.no OI Wilcox, Allen/0000-0002-3376-1311 FU University of Bergen; Western Norway Regional Health Authority; Intramural Research Program of the National Institute of Environmental Health Sciences, National Institutes of Health; National Institutes of Health (NIH) FX Supported by grants from the University of Bergen, the Western Norway Regional Health Authority, and by the Intramural Research Program of the National Institute of Environmental Health Sciences, National Institutes of Health. Funded by the National Institutes of Health (NIH). NR 22 TC 0 Z9 0 U1 5 U2 5 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 EI 1098-4275 J9 PEDIATRICS JI Pediatrics PD AUG PY 2016 VL 138 IS 2 AR e20160269 DI 10.1542/peds.2016-0269 PG 11 WC Pediatrics SC Pediatrics GA DS7ZP UT WOS:000381002500031 ER PT J AU Schonfeld, T Nelson, DK AF Schonfeld, Toby Nelson, Daniel K. TI Reflections From Fellow Feds: Addressing Delays in Oversight of Federally Funded Research SO AMERICAN JOURNAL OF BIOETHICS LA English DT Editorial Material C1 [Schonfeld, Toby; Nelson, Daniel K.] US EPA, Washington, DC 20460 USA. RP Schonfeld, T (reprint author), US EPA, Off Sci Advisor, 1200 Penn Ave NW,Mail Code 8105R, Washington, DC 20460 USA. EM schonfeld.toby@epa.gov NR 9 TC 0 Z9 0 U1 0 U2 0 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1526-5161 EI 1536-0075 J9 AM J BIOETHICS JI Am. J. Bioeth. PD AUG PY 2016 VL 16 IS 8 BP 50 EP 52 DI 10.1080/15265161.2016.1187216 PG 3 WC Ethics; Medical Ethics; Social Issues; Social Sciences, Biomedical SC Social Sciences - Other Topics; Medical Ethics; Social Issues; Biomedical Social Sciences GA DS6OI UT WOS:000380901100021 PM 27366852 ER PT J AU Palazzi, X Burkhardt, JE Caplain, H Dellarco, V Fant, P Foster, JR Francke, S Germann, P Groters, S Harada, T Harleman, J Inui, K Kaufmann, W Lenz, B Nagai, H Pohlmeyer-Esch, G Schulte, A Skydsgaard, M Tomlinson, L Wood, CE Yoshida, M AF Palazzi, Xavier Burkhardt, John E. Caplain, Henri Dellarco, Vicki Fant, Pierluigi Foster, John R. Francke, Sabine Germann, Paul Groeters, Sibylle Harada, Takanori Harleman, Johannes Inui, Kosei Kaufmann, Wolfgang Lenz, Barbara Nagai, Hirofumi Pohlmeyer-Esch, Gabriele Schulte, Agnes Skydsgaard, Mikala Tomlinson, Lindsay Wood, Charles E. Yoshida, Midori TI Characterizing "Adversity" of Pathology Findings in Nonclinical Toxicity Studies: Results from the 4th ESTP International Expert Workshop SO TOXICOLOGIC PATHOLOGY LA English DT Review DE adversity; adverse effect; toxicologic pathology; LOAEL; NOAEL; hazard identification ID RISK-ASSESSMENT; TOXICOLOGIC PATHOLOGY; OUTCOME PATHWAYS; IPCS FRAMEWORK; POSITION PAPER; LESIONS; MODE; RATS; NEPHROPATHY; ADAPTATION AB The identification of adverse health effects has a central role in the development and risk/safety assessment of chemical entities and pharmaceuticals. There is currently a need for better alignment regarding how nonclinical adversity is determined and characterized. The European Society of Toxicologic Pathology (ESTP) therefore coordinated a workshop to review available definitions of adversity, weigh determining and qualifying factors of adversity based on case examples, and recommend a practical approach to define and characterize adversity in toxicology reports, to serve as a valuable prerequisite for future organ- or lesion-specific workshops planned by the ESTP. C1 [Palazzi, Xavier; Caplain, Henri] Sanofi, Vitry Sur Seine, France. [Burkhardt, John E.; Tomlinson, Lindsay] Pfizer Inc, Groton, CT 06340 USA. [Fant, Pierluigi] WIL Res, Lyon, France. [Foster, John R.] Tox Path Sci, Congleton, Cheshire, England. [Francke, Sabine] US FDA, College Pk, MD USA. [Germann, Paul] AbbVie, Ludwigshafen, Germany. [Groeters, Sibylle] BASF, Ludwigshafen, Germany. [Harada, Takanori] Inst Environm Toxicol, Ibaraki, Japan. [Harleman, Johannes] Fresenius Kabi, Bad Homburg, Germany. [Inui, Kosei] Ishihara Sangyo Kaisha Ltd, Osaka, Japan. [Kaufmann, Wolfgang] Merck, Darmstadt, Germany. [Lenz, Barbara] Roche Pharmaceut Res & Early Dev, Basel, Switzerland. [Nagai, Hirofumi] Takeda Pharmaceut Co Ltd, Fujisawa, Kanagawa, Japan. [Pohlmeyer-Esch, Gabriele] Kaleidis Consultancy, St Louis, France. [Schulte, Agnes] Fed Inst Risk Assessment, Berlin, Germany. [Skydsgaard, Mikala] Novo Nordisk AS, Malov, Denmark. [Wood, Charles E.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Yoshida, Midori] Food Safety Commiss, Cabinet Off, Tokyo, Japan. RP Palazzi, X (reprint author), Sanofi R&D, 13 Quai Jules Guesde, F-94400 Vitry Sur Seine, France. EM xavier.palazzi@sanofi.com FU ESTP FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This workshop was coordinated and supported by ESTP and hosted by Sanofi (Centre de Recherche de Vitry-Alfortville, France). NR 46 TC 3 Z9 3 U1 3 U2 3 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0192-6233 EI 1533-1601 J9 TOXICOL PATHOL JI Toxicol. Pathol. PD AUG PY 2016 VL 44 IS 6 BP 810 EP 824 DI 10.1177/0192623316642527 PG 15 WC Pathology; Toxicology SC Pathology; Toxicology GA DS7ZE UT WOS:000381001300002 PM 27102650 ER PT J AU Vane, L Namboodiri, V Lin, G Abar, M Alvarez, F AF Vane, Leland Namboodiri, Vasudevan Lin, Gui Abar, Michael Alvarez, Franklin TI Preparation of Water-Selective Polybutadiene Membranes and Their Use in Drying Alcohols by Pervaporation and Vapor Permeation Technologies SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Pervaporation; Vapor permeation; Azeotrope separation; Membrane-based separation; Solvent drying; Water-selective membrane ID ETHANOL DEHYDRATION; HYDROPHILIC MEMBRANES; POLY(VINYL ALCOHOL); COMPOSITE MEMBRANES; POLYMERIC MEMBRANES; SEPARATION; MIXTURES; PHOTOOXIDATION; 1,2-POLYBUTADIENE; PERFORMANCE AB Separating azeotrope-forming solvent water mixtures by conventional distillation poses technical, economic, and environmental challenges. Pervaporation and vapor permeation membrane technologies using water-permselective membranes provide an efficient alternative for water removal from solvents. We present here new water-selective materials, based on 1,2-polybutadiene, that address two problems reported for traditional hydrophilic membrane materials under high water activities: swelling and hydrolysis. Exposure to UV radiation and/or heat converted portions of the vinyl groups in the polybutadiene to cross-links and hydrophilic functional groups, including alcohols, ketones, and carboxylic acids. In testing with a series of alcohols, such materials displayed high water permeabilities and stable performance over several months even at the extremes of 100% water, low water (2%), and an ethanol/water vapor at 115 degrees C and 2.5 bar. C1 [Vane, Leland; Namboodiri, Vasudevan; Alvarez, Franklin] US EPA, Natl Risk Management Res Lab, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Lin, Gui] CNR, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Abar, Michael] NCOA, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Lin, Gui] Emerson Climate Technol, 1675 West Campbell Rd, Sidney, OH 45365 USA. RP Vane, L (reprint author), US EPA, Natl Risk Management Res Lab, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM Vane.Leland@EPA.gov FU National Research Council FX This work was part of the US EPA's Sustainable and Healthy Communities research program as well as a Cooperative Research and Development Agreement between the US EPA and Membrane Technology and Research, Inc. (Newark, CA). Financial support from the National Research Council for G.L.'s postdoctoral research at US EPA is gratefully acknowledged. NR 48 TC 0 Z9 0 U1 13 U2 13 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD AUG PY 2016 VL 4 IS 8 BP 4442 EP 4450 DI 10.1021/acssuschemeng.6b01072 PG 9 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DS6CR UT WOS:000380869800045 ER PT J AU Richard, AM Judson, RS Houck, KA Grulke, CM Volarath, P Thillainadarajah, I Yang, CH Rathman, J Martin, MT Wambaugh, JF Knudsen, TB Kancherla, J Mansouri, K Patlewicz, G Williams, AJ Little, SB Crofton, KM Thomas, RS AF Richard, Ann M. Judson, Richard S. Houck, Keith A. Grulke, Christopher M. Volarath, Patra Thillainadarajah, Inthirany Yang, Chihae Rathman, James Martin, Matthew T. Wambaugh, John F. Knudsen, Thomas B. Kancherla, Jayaram Mansouri, Kamel Patlewicz, Grace Williams, Antony J. Little, Stephen B. Crofton, Kevin M. Thomas, Russell S. TI ToxCast Chemical Landscape: Paving the Road to 21st Century Toxicology SO CHEMICAL RESEARCH IN TOXICOLOGY LA English DT Article ID IN-VITRO ASSAYS; ENVIRONMENTAL CHEMICALS; CHEMISTRY DATABASES; DRUG DISCOVERY; TOXICITY; CARCINOGENICITY; PROTECTION; KNOWLEDGE; RESOURCE; RECEPTOR AB The U.S. Environmental Protection Agency's (EPA) ToxCast program is testing a large library of Agency-relevant chemicals using in vitro high-throughput screening (HTS) approaches to support the development of improved toxicity prediction models. Launched in 2007, Phase I of the program screened 310 chemicals, mostly pesticides, across hundreds of ToxCast assay end points. In Phase II, the ToxCast library was expanded to 1878 chemicals, culminating in the public release of screening data at the end of 2013. Subsequent expansion in Phase III has resulted in more than 3800 chemicals actively undergoing ToxCast screening, 96% of which are also being screened in the multi-Agency Tox21 project. The chemical library unpinning these efforts plays a central role in defining the scope and potential application of ToxCast HTS results. The history of the phased construction of EPA's ToxCast library is reviewed,, followed by a survey of the library contents from several different vantage points. CAS Registry Numbers are used to assess ToxCast library coverage of important toxicity, regulatory, and exposure inventories. Structure-based representations of ToxCast chemicals are then used to compute physicochemical properties, substructural features, and structural alerts for toxicity and biotransformation. Cheminformatics approaches using these varied representations are applied to defining the boundaries of HTS testability, evaluating chemical diversity, and comparing the ToxCast library to potential target application inventories, such as used in EPA's Endocrine Disruption Screening Program (EDSP). Through several examples, the ToxCast chemical library is demonstrated to provide comprehensive coverage of the knowledge domains and target inventories of potential interest to EPA. Furthermore, the varied representations and approaches presented here define local chemistry domains potentially worthy of further investigation (e.g., not currently covered in the testing library or defined by toxicity "alerts") to strategically support data mining and predictive toxicology modeling moving forward. C1 [Richard, Ann M.; Judson, Richard S.; Houck, Keith A.; Grulke, Christopher M.; Martin, Matthew T.; Wambaugh, John F.; Knudsen, Thomas B.; Patlewicz, Grace; Williams, Antony J.; Little, Stephen B.; Crofton, Kevin M.; Thomas, Russell S.] US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Mail Code B205-01, Durham, NC 27711 USA. [Volarath, Patra] US FDA, Ctr Food Safety & Nutr, 5100 Paint Branch Pkwy, College Pk, MD 20740 USA. [Thillainadarajah, Inthirany] US EPA, Senior Environm Employment Program, Res Triangle Pk, NC 27711 USA. [Yang, Chihae] Mol Networks GmbH, Henkestr 91, D-91052 Erlangen, Germany. [Yang, Chihae; Rathman, James] Altamira LLC, 1455 Candlewood Dr, Columbus, OH 43235 USA. [Rathman, James] Ohio State Univ, Dept Chem & Biomol Engn, 151 W Woodruff Ave, Columbus, OH 43210 USA. [Kancherla, Jayaram; Mansouri, Kamel] US EPA, Durham, NC 27711 USA. [Richard, Ann M.] US EPA, Mail Drop D143-02, Res Triangle Pk, NC 27711 USA. RP Richard, AM (reprint author), US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Mail Code B205-01, Durham, NC 27711 USA.; Richard, AM (reprint author), US EPA, Mail Drop D143-02, Res Triangle Pk, NC 27711 USA. EM richard.ann@epa.gov OI Wambaugh, John/0000-0002-4024-534X; Mansouri, Kamel/0000-0002-6426-8036 FU U.S. Environmental Protection Agency FX The work presented in this manuscript was solely supported by the U.S. Environmental Protection Agency appropriated funds. NR 55 TC 3 Z9 3 U1 20 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0893-228X EI 1520-5010 J9 CHEM RES TOXICOL JI Chem. Res. Toxicol. PD AUG PY 2016 VL 29 IS 8 BP 1225 EP 1251 DI 10.1021/acs.chemrestox.6b00135 PG 27 WC Chemistry, Medicinal; Chemistry, Multidisciplinary; Toxicology SC Pharmacology & Pharmacy; Chemistry; Toxicology GA DT6KQ UT WOS:000381593500001 PM 27367298 ER PT J AU Auerbach, S Filer, D Reif, D Walker, V Holloway, AC Schlezinger, J Srinivasan, S Svoboda, D Judson, R Bucher, JR Thayer, KA AF Auerbach, Scott Filer, Dayne Reif, David Walker, Vickie Holloway, Alison C. Schlezinger, Jennifer Srinivasan, Supriya Svoboda, Daniel Judson, Richard Bucher, John R. Thayer, Kristina A. TI Prioritizing Environmental Chemicals for Obesity and Diabetes Outcomes Research: A Screening Approach Using ToxCast (TM) High-Throughput Data SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Review ID PANCREATIC BETA-CELLS; INSULIN-SECRETION; RODENTICIDE VACOR; MATERNAL SMOKING; WEIGHT-GAIN; C-ELEGANS; FORMAMIDINE PESTICIDE; TRIPHENYLTIN IMPAIRS; OBESOGEN TRIBUTYLTIN; GLUCOSE-INTOLERANCE AB BACKGROUND: Diabetes and obesity are major threats to public health in the United States and abroad. Understanding the role that chemicals in our environment play in the development of these conditions is an emerging issue in environmental health, although identifying and prioritizing chemicals for testing beyond those already implicated in the literature is challenging. This review is intended to help researchers generate hypotheses about chemicals that may contribute to diabetes and to obesity-related health outcomes by summarizing relevant findings from the U.S. Environmental Protection Agency (EPA) ToxCast (TM) high-throughput screening (HTS) program. OBJECTIVES: Our aim was to develop new hypotheses around environmental chemicals of potential interest for diabetes-or obesity-related outcomes using high-throughput screening data. METHODS: We identified ToxCast (TM) assay targets relevant to several biological processes related to diabetes and obesity (insulin sensitivity in peripheral tissue, pancreatic islet and beta cell function, adipocyte differentiation, and feeding behavior) and presented chemical screening data against those assay targets to identify chemicals of potential interest. DISCUSSION: The results of this screening-level analysis suggest that the spectrum of environmental chemicals to consider in research related to diabetes and obesity is much broader than indicated by research papers and reviews published in the peer-reviewed literature. Testing hypotheses based on ToxCast (TM) data will also help assess the predictive utility of this HTS platform. CONCLUSIONS: More research is required to put these screening-level analyses into context, but the information presented in this review should facilitate the development of new hypotheses. C1 [Auerbach, Scott; Walker, Vickie; Bucher, John R.; Thayer, Kristina A.] NIEHS, Div Natl Toxicol Program, NIH, Dept Hlth & Human Serv, POB 12233, Res Triangle Pk, NC 27709 USA. [Filer, Dayne; Judson, Richard] US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Reif, David] North Carolina State Univ, Bioinformat Res Ctr, Dept Biol Sci, Raleigh, NC USA. [Holloway, Alison C.] McMaster Univ, Dept Obstet & Gynecol, Hamilton, ON, Canada. [Schlezinger, Jennifer] Boston Univ, Sch Med, Dept Environm Hlth, Boston, MA 02118 USA. [Srinivasan, Supriya] Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA. [Svoboda, Daniel] SciOme LLC, Res Triangle Pk, NC USA. RP Thayer, KA (reprint author), NIEHS, NTP Off Hlth Assessment & Translat OHAT, NTP, 530 Davis Dr,Room 2150 Mail Drop K2-04, Morrisville, NC 27560 USA. EM thayer@niehs.nih.gov FU National Institute of Environmental Health Sciences/National Institutes of Health; U.S. Environmental Protection Agency (EPA) FX This research was supported by the National Institute of Environmental Health Sciences/National Institutes of Health and the U.S. Environmental Protection Agency (EPA). NR 96 TC 2 Z9 2 U1 6 U2 10 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD AUG PY 2016 VL 124 IS 8 BP 1141 EP 1154 DI 10.1289/ehp.1510456 PG 14 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DS4KL UT WOS:000380749900012 PM 26978842 ER PT J AU Schwarz, K Herrmann, DL AF Schwarz, Kirsten Herrmann, Dustin L. TI The subtle, yet radical, shift to ecology for cities SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Letter ID SOCIETY; SCIENCE C1 [Schwarz, Kirsten] Northern Kentucky Univ, Highland Hts, KY 41099 USA. [Herrmann, Dustin L.] US EPA, Cincinnati, OH 45268 USA. RP Schwarz, K (reprint author), Northern Kentucky Univ, Highland Hts, KY 41099 USA. EM schwarzk1@nku.edu NR 6 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD AUG PY 2016 VL 14 IS 6 BP 296 EP 297 DI 10.1002/fee.1288 PG 2 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DS4KD UT WOS:000380749100010 ER PT J AU Hayes, CR Croft, N Phillips, E Craik, S Schock, M AF Hayes, Colin R. Croft, Nick Phillips, Edith Craik, Steve Schock, Michael TI An evaluation of sampling methods and supporting techniques for tackling lead in drinking water in Alberta Province SO JOURNAL OF WATER SUPPLY RESEARCH AND TECHNOLOGY-AQUA LA English DT Article DE drinking water safety plans; lead in drinking water; plumbosolvency; risk assessment; sampling ID OPTIMIZATION AB A demonstration project evaluated a range of sampling methods and supporting techniques for tackling lead in drinking water in Alberta Province, with the cities of Calgary and Edmonton as case studies. The sampling protocols specified by Health Canada in their 2009 guidance were confirmed to need further improvement and clarification; these sampling protocols produce results that are subject to variable influences and do not provide a sufficiently clear basis for identifying corrosion control needs nor for demonstrating the success or otherwise of mitigation measures. Instead, it was concluded that a risk assessment and risk management approach would be better suited to tackling the lead in drinking water problem in Canadian cities and townships. This can be applied in a more pragmatic manner that reflects the circumstances of individual water supply systems, using drinking water safety plans as the foundation. C1 [Hayes, Colin R.] WQM Associates Ltd, Llanelli, Camarthenshire, Wales. [Croft, Nick] Swansea Univ, Coll Engn, Swansea, W Glam, Wales. [Craik, Steve] EPCOR Water Canada, Qual Assurance & Environm, Edmonton, AB, Canada. [Schock, Michael] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. RP Hayes, CR (reprint author), WQM Associates Ltd, Llanelli, Camarthenshire, Wales. EM c.r.hayes@swansea.ac.uk FU Government of Alberta; City of Calgary; EPCOR FX The project was funded by the Government of Alberta, the City of Calgary and EPCOR. Thanks to Dr Donald Reid for his vision and support. Thanks to the laboratory staff at EPCOR and the City of Calgary for being so patient in providing so much data and supporting information. Team Alberta! NR 15 TC 0 Z9 0 U1 5 U2 5 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 0003-7214 EI 1365-2087 J9 J WATER SUPPLY RES T JI J. Water Supply Res Technol.-Aqua PD AUG PY 2016 VL 65 IS 5 BP 373 EP 383 DI 10.2166/aqua.2016.117 PG 11 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA DS5RW UT WOS:000380841000001 ER PT J AU Hellmann-Blumberg, U Steenson, RA Brewer, RC Allen, E AF Hellmann-Blumberg, Uta Steenson, Ross A. Brewer, Roger C. Allen, Elizabeth TI Toxicity of polar metabolites associated with petroleum hydrocarbon biodegradation in groundwater SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Letter ID FUEL RELEASE SITES; ORGANIC-ACIDS; IN-VITRO; OIL; MIXTURES; TPHD/DRO C1 [Hellmann-Blumberg, Uta] Cal EPA, Dept Tox Subst Control, Sacramento, CA 95812 USA. [Steenson, Ross A.] Cal EPA, San Francisco Bay Reg Water Qual Control Board, Oakland, CA USA. [Brewer, Roger C.] Hawaii Dept Hlth, Off Hazard Evaluat & Emergency Response, Honolulu, HI USA. [Allen, Elizabeth] US Environm Protect Agcy Reg 10, Seattle, WA USA. RP Hellmann-Blumberg, U (reprint author), Cal EPA, Dept Tox Subst Control, Sacramento, CA 95812 USA. NR 16 TC 1 Z9 1 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD AUG PY 2016 VL 35 IS 8 BP 1900 EP 1901 DI 10.1002/etc.3463 PG 2 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DR7CH UT WOS:000380057100002 PM 27442158 ER PT J AU Kapoor, V Elk, M Li, X Domingo, JWS AF Kapoor, V. Elk, M. Li, X. Domingo, J. W. Santo TI Inhibitory effect of cyanide on wastewater nitrification determined using SOUR and RNA-based gene-specific assays SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE ammonia-oxidizing bacteria; cyanide; nitrification; reverse transcriptase-qPCR; substrate (ammonia) specific oxygen uptake rates; wastewater ID NITROSOMONAS-EUROPAEA; TRANSCRIPTIONAL RESPONSES; ACTIVATED-SLUDGE; MICROBIAL COMMUNITIES; NITRIFYING BACTERIA; HEAVY-METALS; EXPRESSION; REMOVAL; CR(VI); CARBON AB The effect of cyanide (CN-) on nitrification was examined with samples from nitrifying bacterial enrichments using two different approaches: by measuring substrate (ammonia) specific oxygen uptake rates (SOUR), and by using RT-qPCR to quantify the transcripts of functional genes involved in nitrification. The nitrifying bioreactor was operated as a continuous reactor with a 24h hydraulic retention time. The samples were exposed in batch vessels to cyanide for a period of 12h. The concentrations of CN- used in the batch assays were 003, 006, 01 and 10mgl(-1). There was considerable decrease in SOUR with increasing dosages of CN-. A decrease of more than 50% in nitrification activity was observed at 01mgl(-1) CN-. Based on the RT-qPCR data, there was notable reduction in the transcript levels of amoA and hao for increasing CN- dosage, which corresponded well with the ammonia oxidation activity measured via SOUR. The inhibitory effect of cyanide may be attributed to the affinity of cyanide to bind ferric haeme proteins, which disrupt protein structure and function. The correspondence between the relative expression offunctional genes and SOUR shown in this study demonstrates the efficacy of RNA-based function-specific assays for better understanding of the effect oftoxic compounds on nitrification activity in wastewater. C1 [Kapoor, V.; Li, X.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Kapoor, V.; Li, X.; Domingo, J. W. Santo] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. [Elk, M.] Pegasus Tech Serv Inc, Cincinnati, OH USA. RP Domingo, JWS (reprint author), US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. EM santodomingo.jorge@epa.gov FU U. S. Environmental Protection Agency (EPA) FX Vikram Kapoor and Xuan Li were supported by U. S. Environmental Protection Agency (EPA) via a post-doctoral appointment administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U. S. Department of Energy and the U. S. EPA. The manuscript has been subjected to the EPA's peer review and has been approved as an EPA publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by the EPA for use. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U. S. Environmental Protection Agency. NR 31 TC 0 Z9 0 U1 3 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD AUG PY 2016 VL 63 IS 2 BP 155 EP 161 DI 10.1111/lam.12603 PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DR7BP UT WOS:000380055300012 PM 27281632 ER PT J AU Datry, T Fritz, K Leigh, C AF Datry, Thibault Fritz, Ken Leigh, Catherine TI Challenges, developments and perspectives in intermittent river ecology SO FRESHWATER BIOLOGY LA English DT Article DE drying; flow cessation; ephemeral; intermittent; temporary; rivers; climate change; genealogy ID TEMPORARY RIVERS; STREAM FISHES; MACROINVERTEBRATE ASSEMBLAGES; ENVIRONMENTAL VARIABILITY; AQUATIC INVERTEBRATES; COMMUNITY STRUCTURE; FLOW VARIABILITY; WATER-RESOURCES; HYPORHEIC ZONE; RUNNING WATERS AB Although more than half the world's river networks comprise channels that periodically cease to flow and dry [intermittent rivers (IRs)], river ecology was largely developed from and for perennial systems. Ecological knowledge of IRs is rapidly increasing, so there is a need to synthesise this knowledge and deepen ecological understanding. In this Special Issue, we bring together 13 papers spanning observational case studies, field and laboratory experiments and reviews to guide research and management in this productive field of freshwater science. We summarise new developments in IR ecology, identify research gaps and needs, and address how the study of IRs as highly dynamic ecosystems informs ecological understanding more broadly. This series of articles reveals that contemporary IR ecology is a multifaceted and maturing field of research at the interface between aquatic and terrestrial ecology. This research contributes to fresh water and general ecology by testing concepts across a range of topics, including disturbance ecology, metacommunity ecology and coupled aquatic-terrestrial ecosystems. Drying affects flow continuity through time and flow connectivity across longitudinal, lateral and vertical dimensions of space, which aligns well with the recent emphasis of mainstream ecology on meta-system perspectives. Although most articles here focus on the wet phase, there is growing interest in dry phases, and in the terrestrial vegetation and invertebrate assemblages living in and along IR channels. We encourage interdisciplinary studies on IRs to further blur the demarcation between aquatic and terrestrial ecosystems and develop more integrated perspectives. As a result of climate change and human modification of landscapes and waterways, flooding and drought are expected to become more extreme and widespread. Shifts in streamflow regimes from perennial to intermittent may exacerbate the duration and frequency of dry phases in IRs with serious implications for river ecosystems and the quality and diversity of services they provide. C1 [Datry, Thibault; Leigh, Catherine] Ctr Lyon Villeurbanne, IRSTEA, UR MALY, 5 Rue Doua CS70077, F-69626 Villeurbanne, France. [Datry, Thibault] Museum Natl Hist Nat, UMR BOREA CNRS IRD MNHN UPMC 7208 207, DMPA, Paris, France. [Fritz, Ken] US EPA, Off Res & Dev, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [Leigh, Catherine] CESAB FRB Immeuble Henri Poincare, Aix En Provence 3, France. [Leigh, Catherine] Griffith Univ, Australian Rivers Inst, 170 Kessels Rd, Nathan, Qld 4111, Australia. RP Datry, T (reprint author), Ctr Lyon Villeurbanne, IRSTEA, UR MALY, 5 Rue Doua CS70077, F-69626 Villeurbanne, France. EM thibault.datry@irstea.fr RI Leigh, Catherine/B-8868-2009 OI Leigh, Catherine/0000-0003-4186-1678 FU French Foundation for Research on Biodiversity; French National Agency for Water & Aquatic Environments in the context of the CESAB project 'Intermittent River Biodiversity Analysis and Synthesis' (IRBAS) FX TD and CL were supported by the French Foundation for Research on Biodiversity and the French National Agency for Water & Aquatic Environments in the context of the CESAB project 'Intermittent River Biodiversity Analysis and Synthesis' (IRBAS; www.irbas.fr). We thank the many colleagues and friends who have been involved in IRBAS, including Andrew Boulton, Nuria Bonada, Cliff Dahm, Bernard Dumont, Bernard Hugueny, Scott Larned, Eric Sauquet and Klement Tockner. We warmly thank David Strayer for his guidance and support as Special Issues Editor for Freshwater Biology, all of the authors for their contributions to the Issue, and the many who reviewed manuscripts for us. We also thank Angela Arthington and Ken Forshay for providing valuable comments on early drafts of this introductory paper. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. NR 84 TC 2 Z9 2 U1 29 U2 55 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0046-5070 EI 1365-2427 J9 FRESHWATER BIOL JI Freshw. Biol. PD AUG PY 2016 VL 61 IS 8 SI SI BP 1171 EP 1180 DI 10.1111/fwb.12789 PG 10 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA DR5PB UT WOS:000379953800001 ER PT J AU Leigh, C Boulton, AJ Courtwright, JL Fritz, K May, CL Walker, RH Datry, T AF Leigh, Catherine Boulton, Andrew J. Courtwright, Jennifer L. Fritz, Ken May, Christine L. Walker, Richard H. Datry, Thibault TI Ecological research and management of intermittent rivers: an historical review and future directions SO FRESHWATER BIOLOGY LA English DT Review DE biogeochemistry; ephemeral stream; fish; invertebrate; temporary river ID SONORAN DESERT STREAM; FISH COMMUNITY STRUCTURE; MEDITERRANEAN-CLIMATE STREAMS; WATER-RESOURCES MANAGEMENT; AUSTRALIAN DRYLAND RIVERS; SOUTH-EASTERN AUSTRALIA; SAN-PEDRO RIVER; TEMPORARY STREAMS; MACROINVERTEBRATE ASSEMBLAGES; DRY-SEASON AB Rivers and streams that do not flow permanently (herein intermittent rivers; IRs) make up a large proportion of the world's inland waters and are gaining widespread attention. We review the research on IRs from its early focus on natural history through to current application in management and policy. The few early studies of the ecology of IRs were largely descriptive. Nevertheless, in the 1970s, synthesis of this sparse research complemented work on temporary standing waters to found a powerful framework for much of the subsequent research on IRs. Research on the ecology and biogeochemistry of IRs continues to fuel our understanding of resistance and resilience to drying and flooding as disturbances. Syntheses of the growing literature, including cross-continental and cross-climate comparisons, are revealing the generality and individuality of ecological and ecosystem responses to flow cessation and surface water loss. Meanwhile, increasing numbers of experiments test the causality of these responses. Much of the increased consideration of IRs in research, management and policy is driven by the observed and projected shifts in flow regimes from perennial to intermittent associated with changes in land and water use and climate, superimposed on the high incidence of natural intermittency. The need to protect and better manage IRs is prompting researchers to develop new or modified methods to monitor flow status and assess the ecological condition of these systems. Intermittent river research and management will benefit from greater exploration of aquatic-terrestrial linkages, wet-dry cycling and temporal dynamics, more-detailed mapping and predictive modelling of flow intermittency and the application of metapopulation and metacommunity concepts alongside multiple-stressors and novel-ecosystems research. By building on existing knowledge, continuing to develop quantitative models and distribution maps and using experiments to test hypotheses and concepts, we can further ecological understanding and wise management of these ubiquitous ecosystems. C1 [Leigh, Catherine; Datry, Thibault] Ctr Lyon Villeurbanne, Irstea, UR MALY, 5 Rue Doua CS70077, F-69626 Villeurbanne, France. [Leigh, Catherine] Immeuble Henri Poincare, CESAB FRB, Aix En Provence, France. [Boulton, Andrew J.] Univ New England, Ecosyst Management, Sch Environm & Rural Sci, Armidale, NSW, Australia. [Courtwright, Jennifer L.] Utah State Univ, US Bur Land Management, Natl Aquat Monitoring Ctr, Dept Watershed Sci, Logan, UT 84322 USA. [Fritz, Ken] US EPA, Off Res & Dev, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [May, Christine L.] James Madison Univ, Dept Biol, Harrisonburg, VA 22807 USA. [Walker, Richard H.] Univ Wyoming, Dept Zool & Physiol, Wyoming Cooperat Fish & Wildlife Res Unit, Laramie, WY 82071 USA. [Datry, Thibault] Museum Natl Hist Nat, UMR BOREA CNRS IRD MNHN UPMC 7208 207, DMPA, Paris, France. RP Leigh, C (reprint author), Ctr Lyon Villeurbanne, Irstea, UR MALY, 5 Rue Doua CS70077, F-69626 Villeurbanne, France. EM catherine.leigh@irstea.fr OI Leigh, Catherine/0000-0003-4186-1678 FU IRBAS (Intermittent River Biodiversity Analysis and Synthesis working group); Centre for Synthesis and Analysis of Biodiversity (CESAB); French Foundation for Research AMP; Biodiversity (FRB); French National Agency for Water and Aquatic Environments (ONEMA); CESAB FX Earlier versions of this manuscript were improved by Cliff Dahm, Nicolas Lamouroux, Fran Sheldon, Dave Strayer and two anonymous reviewers. CL was funded by IRBAS (Intermittent River Biodiversity Analysis and Synthesis working group; www.irbas.fr), which is supported by the Centre for Synthesis and Analysis of Biodiversity (CESAB), and funded jointly by the French Foundation for Research & Biodiversity (FRB) and the French National Agency for Water and Aquatic Environments (ONEMA). We thank CESAB for supporting IRBAS workshops at which ideas on the subject of this paper were discussed, and the Australian Rivers Institute, Griffith University, for providing CL library access. TD and CL are participants of IRBAS; AB and KF are affiliates. We thank Herve Pella for producing the world maps in Figure S1b. The views expressed in this journal article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. NR 214 TC 15 Z9 15 U1 37 U2 54 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0046-5070 EI 1365-2427 J9 FRESHWATER BIOL JI Freshw. Biol. PD AUG PY 2016 VL 61 IS 8 SI SI BP 1181 EP 1199 DI 10.1111/fwb.12646 PG 19 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA DR5PB UT WOS:000379953800002 ER PT J AU Druwe, IL Burgoon, L AF Druwe, Ingrid L. Burgoon, Lyle TI Revisiting Cohen et al. 2015, Cohen et al. 2014 and Waalkes et al. 2014: a bayesian re-analysis of tumor incidences SO ARCHIVES OF TOXICOLOGY LA English DT Letter ID EXPOSURE; MICE C1 [Druwe, Ingrid L.] US EPA, Oak Ridge Inst Sci & Educ, Natl Ctr Environm Assessment, Vicksburg, MS USA. [Burgoon, Lyle] US Army, Engineer Res & Dev Ctr, Environm Lab, Res Triangle Pk, NC 27709 USA. RP Burgoon, L (reprint author), US Army, Engineer Res & Dev Ctr, Environm Lab, Res Triangle Pk, NC 27709 USA. EM lyle.d.burgoon@usace.army.mil OI Burgoon, Lyle/0000-0003-4977-5352 NR 3 TC 3 Z9 3 U1 0 U2 1 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0340-5761 EI 1432-0738 J9 ARCH TOXICOL JI Arch. Toxicol. PD AUG PY 2016 VL 90 IS 8 BP 2047 EP 2048 DI 10.1007/s00204-016-1749-0 PG 2 WC Toxicology SC Toxicology GA DQ5OK UT WOS:000379254500021 PM 27325233 ER PT J AU Walsh, PJ Milon, JW AF Walsh, Patrick J. Milon, J. Walter TI Nutrient Standards, Water Quality Indicators, and Economic Benefits from Water Quality Regulations SO ENVIRONMENTAL & RESOURCE ECONOMICS LA English DT Article DE Hedonic analysis; Benefit-cost analysis; Spatial econometrics; Water quality ID FRESH-WATER; ECOSYSTEM SERVICES; URBAN WATERSHEDS; PROPERTY-VALUES; IMPLICIT PRICES; UNITED-STATES; VALUATION; MODELS; LAKES; FLORIDA AB There is no consensus in the hedonic property pricing literature on measures of water quality to use for regulatory policy analysis. This study compares several alternative measures of water quality with a focus on singular and composite nutrient indicators. Our contribution is to compare and contrast these indicators in the context of benefit analysis based on recent regulatory programs for nutrients in the US and EU. Results indicate order of magnitude differences in the benefits derived from the different types of indicators. We find support for a compound indicator that combines three policy-relevant indicators into an overall measure of waterbody health and is significantly related to property values. Given the growing interest in objective criteria for regulating nutrients and other nonpoint source pollutants, these results provide guidance on the selection of indicators in property valuation studies of water quality regulations. C1 [Walsh, Patrick J.] US EPA, Natl Ctr Environm Econ, US EPA Headquarters, 1200 Penn Ave,NW,WJC Bldg MC 1809T, Washington, DC 20460 USA. [Milon, J. Walter] Univ Cent Florida, Dept Econ, UCF Coll Business Adm, 4000 Cent Florida Blvd,POB 161400, Orlando, FL 32816 USA. RP Walsh, PJ (reprint author), US EPA, Natl Ctr Environm Econ, US EPA Headquarters, 1200 Penn Ave,NW,WJC Bldg MC 1809T, Washington, DC 20460 USA. EM Walsh.Patrick@epa.gov; wmilon@bus.ucf.edu NR 58 TC 0 Z9 0 U1 14 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0924-6460 EI 1573-1502 J9 ENVIRON RESOUR ECON JI Environ. Resour. Econ. PD AUG PY 2016 VL 64 IS 4 BP 643 EP 661 DI 10.1007/s10640-015-9892-2 PG 19 WC Economics; Environmental Studies SC Business & Economics; Environmental Sciences & Ecology GA DQ9OT UT WOS:000379539900006 ER PT J AU Ridley, CE Alexander, LC AF Ridley, Caroline E. Alexander, Laurie C. TI Applying gene flow science to environmental policy needs: a boundary work perspective SO EVOLUTIONARY APPLICATIONS LA English DT Article DE boundary work; environmental policy; geneflow; genetically engineered crops; watershed connectivity ID EVOLUTIONARILY-SIGNIFICANT-UNITS; DISTINCT POPULATION SEGMENTS; COASTAL CUTTHROAT TROUT; ENDANGERED SPECIES ACT; CLIMATE-CHANGE; FRESH-WATER; CREEPING BENTGRASS; LANDSCAPE GENETICS; WILD POPULATIONS; POTENTIAL ROLE AB One application of gene flow science is the policy arena. In this article, we describe two examples in which the topic of gene flow has entered into the U.S. national environmental policymaking process: regulation of genetically engineered crops and clarification of the jurisdictional scope of the Clean Water Act. We summarize both current scientific understanding and the legal context within which gene flow science has relevance. We also discuss the process by which scientific knowledge has been synthesized and communicated to decision-makers in these two contexts utilizing the concept of boundary work'. Boundary organizations, the work they engage in to bridge the worlds of science, policy, and practice, and the boundary objects they produce to translate scientific knowledge existed in both examples. However, the specific activities and attributes of the objects produced varied based on the needs of the decision-makers. We close with suggestions for how scientists can contribute to or engage in boundary work with policymakers. C1 [Ridley, Caroline E.; Alexander, Laurie C.] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Washington, DC 20460 USA. RP Ridley, CE (reprint author), US EPA, Mail Code 8623P,1200 Penn Ave NW, Washington, DC 20460 USA. EM ridley.caroline@epa.gov NR 108 TC 1 Z9 1 U1 14 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1752-4571 J9 EVOL APPL JI Evol. Appl. PD AUG PY 2016 VL 9 IS 7 SI SI BP 924 EP 936 DI 10.1111/eva.12393 PG 13 WC Evolutionary Biology SC Evolutionary Biology GA DR5GW UT WOS:000379932500009 PM 27468309 ER PT J AU Goldsmith, K Granek, E Lubitow, A Papenfus, M AF Goldsmith, Kaitlin Granek, Elise Lubitow, Amy Papenfus, Michael TI Bridge over troubled waters: A synthesis session to connect scientific and decision making sectors SO MARINE POLICY LA English DT Article DE Coastal management; Evidence-based decisions; Research based policy; Stakeholder engagement; Synthesis session; Workshop ID KNOWLEDGE; SCIENCE; GOVERNANCE; WORKSHOP; MAKERS; NEEDS AB Lack of access to relevant scientific data has limited decision makers from incorporating scientific information into their management and policy schemes. Yet, there is increasing interest among decision makers and scientists to integrate coastal and marine science into the policy and management process. Strategies designed to build communication between decision makers and scientists can be an effective means to disseminate and/or generate policy relevant scientific information. Here researchers develop, test, and present a workshop model designed to bridge the gap between coastal and marine decision makers and scientists. Researchers identify successful components of such a workshop as well as areas for improvement and recommendations to design and conduct similar workshops in the future. This novel workshop format can be used in other fora to effectively connect decision makers and scientists, and to initiate an iterative process to generate and transfer policy relevant scientific information into evidence-based decisions, an important element in protecting coastal and marine resources. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Goldsmith, Kaitlin; Granek, Elise] Portland State Univ, Environm Sci & Management, POB 751, Portland, OR 97207 USA. [Lubitow, Amy] Portland State Univ, Sociol, POB 751, Portland, OR 97207 USA. [Papenfus, Michael] US EPA, Off Res & Dev, Western Ecol Div, Corvallis, OR 97330 USA. RP Goldsmith, K (reprint author), Portland State Univ, Environm Sci & Management, POB 751, Portland, OR 97207 USA. EM kaitygoldsmith@gmail.com FU Oregon Sea Grant through the Robert E. Malouf Marine Studies Scholarship FX The research team thanks Oregon Sea Grant for its support of this project through the Robert E. Malouf Marine Studies Scholarship to KG. The research team also thanks all decision makers and scientists who participated in the workshop and helped propel evidence-based decision making forward. NR 24 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-597X EI 1872-9460 J9 MAR POLICY JI Mar. Pol. PD AUG PY 2016 VL 70 BP 30 EP 39 DI 10.1016/j.marpol.2016.04.015 PG 10 WC Environmental Studies; International Relations SC Environmental Sciences & Ecology; International Relations GA DQ7FE UT WOS:000379371500004 ER PT J AU Beggs, KM McGreal, SR McCarthy, A Gunewardena, S Lampe, JN Lau, C Apte, U AF Beggs, Kevin M. McGreal, Steven R. McCarthy, Alex Gunewardena, Sumedha Lampe, Jed N. Lau, Christoper Apte, Udayan TI The role of hepatocyte nuclear factor 4-alpha in perfluorooctanoic acid- and perfluorooctanesulfonic acid-induced hepatocellular dysfunction SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article DE Perfluorooctanoic acid; Perfluorooctanesulfonic acid; Hepatocyte nuclear factor 4-alpha; Human hepatocyte; Liver ID ACTIVATED RECEPTOR-ALPHA; SPRAGUE-DAWLEY RATS; SERUM-LIPID LEVELS; PPAR-ALPHA; PERFLUOROALKYL ACIDS; HEPATIC STEATOSIS; GENE-EXPRESSION; HUMAN RELEVANCE; ADULT MICE; POTASSIUM PERFLUOROOCTANESULFONATE AB Perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS), chemicals present in a multitude of consumer products, are persistent organic pollutants. Both compounds induce hepatotoxic effects in rodents, including steatosis, hepatomegaly and liver cancer. The mechanisms of PFOA- and PFOS-induced hepatic dysfunction are not completely understood. We present evidence that PFOA and PFOS induce their hepatic effects via targeting hepatocyte nuclear factor 4-alpha (HNF4 alpha). Human hepatocytes treated with PFOA and PFOS at a concentration relevant to occupational exposure caused a decrease in HNF4 alpha protein without affecting HNF4 alpha mRNA or causing cell death. RNA sequencing analysis combined with Ingenuity Pathway Analysis of global gene expression changes in human hepatocytes treated with PFOA or PFOS indicated alterations in the expression of genes involved in lipid metabolism and tumorigenesis, several of which are regulated by HNF4 alpha. Further investigation of specific HNF4 alpha target gene expression revealed that PFOA and PFOS could promote cellular dedifferentiation and increase cell proliferation by down regulating positive targets (differentiation genes such as CYP7A1) and inducing negative targets of HNF4 alpha (pro-mitogenic genes such as CCND1). Furthermore, in silico docking simulations indicated that PFOA and PFOS could directly interact with HNF4 alpha in a similar manner to endogenous fatty acids. Collectively, these results highlight HNF4 alpha degradation as novel mechanism of PFOA and PFOS-mediated steatosis and tumorigenesis in human livers. (C) 2016 Elsevier Inc All rights reserved. C1 [Beggs, Kevin M.; McGreal, Steven R.; McCarthy, Alex; Lampe, Jed N.; Apte, Udayan] Univ Kansas, Dept Pharmacol Toxicol & Therapeut, Med Ctr, 3901 Rainbow Blvd,4052 HLSIC, Kansas City, KS 66160 USA. [Gunewardena, Sumedha] Univ Kansas, Dept Mol & Integrat Physiol, Med Ctr, 3901 Rainbow Blvd,2027 HLSIC, Kansas City, KS 66160 USA. [Lau, Christoper] US EPA, Dev Toxicol Branch, Tox Assessment Div, Natl Hlth & Environm Effects Res Lab,Off Res & De, Res Triangle Pk, NC 27711 USA. RP Apte, U (reprint author), Univ Kansas, Dept Pharmacol Toxicol & Therapeut, Med Ctr, 3901 Rainbow Blvd,4052 HLSIC, Kansas City, KS 66160 USA.; Apte, U (reprint author), Univ Kansas, Med Ctr, 3901 Rainbow Blvd,4087 HLSIC, Kansas City, KS 66160 USA. EM kbeggs2@kumc.edu; smcgreal@kumc.edu; sgunewardena@kumc.edu; jlampe@kumc.edu; lau.christopher@epa.gov; uapte@kumc.edu OI Lampe, Jed/0000-0003-0794-2263 FU National Institutes of Health [1R01DK098414, P20 RR021940, T32ES007079-34]; U.S. Environmental Protection Agency FX This work was funded by The National Institutes of Health, grant numbers 1R01DK098414, P20 RR021940 and T32ES007079-34, and partially funded by the U.S. Environmental Protection Agency. The National Institutes of Health had no involvement in the design of this study or the interpretation of its results. The information in this document has been subjected to review by the National Health and Environmental Effects Research Laboratory of US EPA and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. Ken Dorko and Hongmin Ni of the Cell Isolation Core at the University of Kansas Medical Center isolated human hepatocytes for experimental use. NR 75 TC 0 Z9 0 U1 5 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0041-008X EI 1096-0333 J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD AUG 1 PY 2016 VL 304 BP 18 EP 29 DI 10.1016/j.taap.2016.05.001 PG 12 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA DQ8HV UT WOS:000379451700003 PM 27153767 ER PT J AU Fry, MM Rothman, G Young, DF Thurman, N AF Fry, Meridith M. Rothman, Gabriel Young, Dirk F. Thurman, Nelson TI Daily gridded weather for pesticide exposure modeling SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Weather; Pesticide; Exposure; Fate; Transport ID UNITED-STATES; EVAPOTRANSPIRATION; REANALYSIS; SWAT AB Daily weather is compiled for pesticide exposure modeling from 1961 to 2014 at 0.25 x 0.25 degrees latitude/longitude resolution for the United States using two National Oceanic and Atmospheric Administration (NOAA) products: National Center for Environmental Prediction Reanalysis and NOAA Climate Prediction Center Unified Rain Gauge Analysis. The compiled weather includes precipitation, temperature, wind speed, solar radiation, and reference evapotranspiration. Reference evapotranspiration is calculated using the Hargreaves-Samani method. Prior to this update, US pesticide exposure models relied upon the Solar and Meteorological Surface Observation Network dataset, which provides the same variables but only from 1961 to 1990 for 237 US weather stations. More extensive (1961-2014), spatially-resolved weather allows for more robust estimates of time-averaged pesticide concentrations for assessing acute and chronic exposure to pesticides. Continued expansion of the weather dataset is planned as the latest data is released. Processed weather for pesticide exposure modeling will be publicly available from the US EPA. Published by Elsevier Ltd. C1 [Fry, Meridith M.; Rothman, Gabriel; Young, Dirk F.; Thurman, Nelson] US EPA, Off Pesticide Programs, Environm Fate & Effects Div, 1200 Penn Ave NW 7507P, Washington, DC 20460 USA. RP Fry, MM (reprint author), US EPA, Off Pesticide Programs, Environm Fate & Effects Div, 1200 Penn Ave NW 7507P, Washington, DC 20460 USA. EM fry.meridith@epa.gov NR 40 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD AUG PY 2016 VL 82 BP 167 EP 173 DI 10.1016/j.envsoft.2016.04.008 PG 7 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA DQ1IF UT WOS:000378954000013 ER PT J AU Seltzer, KM Nolte, CG Spero, TL Appel, KW Xing, J AF Seltzer, Karl M. Nolte, Christopher G. Spero, Tanya L. Appel, K. Wyat Xing, Jia TI Evaluation of near surface ozone and particulate matter in air quality simulations driven by dynamically downscaled historical meteorological fields SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Air quality; Dynamical downscaling; Model evaluation; Climate change; CMAQ ID CMAQ MODELING SYSTEM; REGIONAL CLIMATE SIMULATIONS; NORTHEASTERN UNITED-STATES; HEALTH IMPACTS; NORTH-AMERICA; PART I; EMISSIONS; TRENDS; US; EUROPE AB In this study, techniques typically used for future air quality projections are applied to a historical 11-year period to assess the performance of the modeling system when the driving meteorological conditions are obtained using dynamical downscaling of coarse-scale fields-without correcting toward higher resolution observations. The Weather Research and Forecasting model and the Community Multiscale Air Quality model are used to simulate regional climate and air quality over the contiguous United States for 2000-2010. The air quality simulations for that historical period are then compared to observations from four national networks. Comparisons are drawn between defined performance metrics and other published modeling results for predicted ozone, fine particulate matter, and speciated fine particulate matter. The results indicate that the historical air quality simulations driven by dynamically downscaled meteorology are typically within defined modeling performance benchmarks and are consistent with results from other published modeling studies using finer-resolution meteorology. This indicates that the regional climate and air quality modeling framework utilized here does not introduce substantial bias, which provides confidence in the method's use for future air quality projections. Published by Elsevier Ltd. C1 [Seltzer, Karl M.; Nolte, Christopher G.; Spero, Tanya L.; Appel, K. Wyat; Xing, Jia] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Seltzer, Karl M.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Xing, Jia] Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China. RP Nolte, CG (reprint author), US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. EM nolte.chris@epa.gov RI Nolte, Christopher/H-4345-2012 OI Nolte, Christopher/0000-0001-5224-9965 FU Research Participation Program at the U.S. Environmental Protection Agency (EPA); National Research Council Research Associateship Award at the U.S. EPA under Department of Energy [DE-SC000378]; EPA [RW-89-9233260] FX Ozone and PM measurement data from the AQS, CSN, IMPROVE, and CASTNet field sites were acquired from the Federal Land Manager Environmental Database at http://views.cira.colostate.edu/fed/DataWizard. K.M.S. was supported by the Research Participation Program at the U.S. Environmental Protection Agency (EPA) administered by the Oak Ridge Institute for Science and Education. J.X. was supported by a National Research Council Research Associateship Award at the U.S. EPA under Department of Energy interagency agreement DE-SC000378 and EPA interagency agreement RW-89-9233260. Comments by Christian Hogrefe (EPA) and two anonymous reviewers served to strengthen this manuscript. Special thanks to Barron Henderson (University of Florida) for providing computational space to perform some of this analysis. The U.S. EPA through its Office of Research and Development collaborated in the research described here. This paper has been subjected to the Agency's administrative review and approved for publication. NR 64 TC 0 Z9 0 U1 5 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD AUG PY 2016 VL 138 BP 42 EP 54 DI 10.1016/j.atmosenv.2016.05.010 PG 13 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DP4FO UT WOS:000378451900005 ER PT J AU Speers, AE Besedin, EY Palardy, JE Moore, C AF Speers, Ann E. Besedin, Elena Y. Palardy, James E. Moore, Chris TI Impacts of climate change and ocean acidification on coral reef fisheries: An integrated ecological-economic model SO ECOLOGICAL ECONOMICS LA English DT Article DE Coral reefs; Ocean acidification; Climate change; Consumer demand for fish; Reef fisheries ID INVERSE DEMAND SYSTEM; FISH COMMUNITIES; BLEACHED CORALS; FOOD SECURITY; FUTURE; RESILIENCE; SUSTAINABILITY; ECOSYSTEMS; DECLINE; MARKET AB Coral reefs are highly productive shallow marine habitats at risk of degradation due to CO2-mediated global ocean changes, including ocean acidification and rising sea temperature. Consequences of coral reef habitat loss are expected to include reduced reef fisheries production. To our knowledge, the welfare impact of reduced reef fish supply in commercial markets has not yet been studied. We develop a global model of annual demand for reef fish in regions with substantial coral reef area and use it to project potential consumer surplus losses given coral cover projections from a coupled climate, ocean, and coral biology simulation (CO2-COST). Under an illustrative high emission scenario (IPCC RCP 8.5), 92% of coral cover is lost by 2100. Policies reaching lower radiative forcing targets (e.g., IPCC RCP 6.0) may partially avoid habitat loss, thereby preserving an estimated $14 to $20 billion in consumer surplus through 2100 (2014$ USD, 3% discount). Avoided damages vary annually, are sensitive to biological assumptions, and appear highest when coral ecosystems have moderate adaptive capacity. These welfare loss estimates are the first to monetize ocean acidification impacts to commercial finfisheries and complement the existing estimates of economic impacts to shellfish and to coral reefs generally. (C) 2016 Elsevier B.V. All rights reserved. C1 [Speers, Ann E.; Besedin, Elena Y.; Palardy, James E.] ABT Associates Inc, Environm & Resources Div, 55 Wheeler St, Cambridge, MA 02138 USA. [Moore, Chris] US EPA, Natl Ctr Environm Econ, 1301 Constitut Ave NW, Washington, DC 20004 USA. [Speers, Ann E.] Opin Dynam, 1000 Winter St, Waltham, MA 02451 USA. RP Speers, AE (reprint author), ABT Associates Inc, Environm & Resources Div, 55 Wheeler St, Cambridge, MA 02138 USA.; Speers, AE (reprint author), Opin Dynam, 1000 Winter St, Waltham, MA 02451 USA. EM aspeers@opiniondynamics.com; elena_besedin@abtassoc.com; james_palardy@abtassoc.com; moore.chris@epa.gov FU U.S. EPA [EP-W-11-003] FX The authors acknowledge helpful comments from Dr. Ellen Post and Dr. Cathy Roheim, and from two anonymous reviewers. U.S. EPA Contract No. EP-W-11-003 to Abt Associates initiated and funded the research and supported article preparation. This study has not been subjected to Agency review. Therefore, the findings, conclusions, and views expressed here are those of the authors and do not necessarily reflect those of the U.S. EPA. No official Agency endorsement should be inferred. NR 108 TC 0 Z9 0 U1 172 U2 292 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8009 EI 1873-6106 J9 ECOL ECON JI Ecol. Econ. PD AUG PY 2016 VL 128 BP 33 EP 43 DI 10.1016/j.ecolecon.2016.04.012 PG 11 WC Ecology; Economics; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology; Business & Economics GA DP7HL UT WOS:000378669700005 ER PT J AU Kuo, B Webster, AF Thomas, RS Yauk, CL AF Kuo, Byron Webster, A. Francina Thomas, Russell S. Yauk, Carole L. TI BMDExpress Data Viewer - a visualization tool to analyze BMDExpress datasets SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE microarray; gene expression; genomics; transcriptomics; benchmark dose (BMD); BMDExpress; bioinformatics; data visualization; human health risk assessment; furan; 4,4 '-methylenebis(N,N-dimethyl)benzenamine (MDMB); dose-response ID CHEMICAL RISK-ASSESSMENT; LARGE GENE LISTS; TECHNOLOGIES; CONCORDANCE; EXPOSURE; POINTS; CANCER; MICE AB Regulatory agencies increasingly apply benchmark dose (BMD) modeling to determine points of departure for risk assessment. BMDExpress applies BMD modeling to transcriptomic datasets to identify transcriptional BMDs. However, graphing and analytical capabilities within BMDExpress are limited, and the analysis of output files is challenging. We developed a web-based application, BMDExpress Data Viewer (), for visualizing and graphing BMDExpress output files. The application consists of Summary Visualization and Dataset Exploratory tools. Through analysis of transcriptomic datasets of the toxicants furan and 4,4-methylenebis(N,N-dimethyl)benzenamine, we demonstrate that the Summary Visualization Tools can be used to examine distributions of gene and pathway BMD values, and to derive a potential point of departure value based on summary statistics. By applying filters on enrichment P-values and minimum number of significant genes, the Functional Enrichment Analysis tool enables the user to select biological processes or pathways that are selectively perturbed by chemical exposure and identify the related BMD. The Multiple Dataset Comparison tool enables comparison of gene and pathway BMD values across multiple experiments (e.g., across timepoints or tissues). The BMDL-BMD Range Plotter tool facilitates the observation of BMD trends across biological processes or pathways. Through our case studies, we demonstrate that BMDExpress Data Viewer is a useful tool to visualize, explore and analyze BMDExpress output files. Visualizing the data in this manner enables rapid assessment of data quality, model fit, doses of peak activity, most sensitive pathway perturbations and other metrics that will be useful in applying toxicogenomics in risk assessment. (c) 2015 Her Majesty the Queen in Right of Canada. Journal of Applied Toxicology published by John Wiley & Sons, Ltd. We developed BMDExpress Data Viewer, which contains two collections of tools, Summary Visualization Tools and Dataset Exploratory Tools, to visualize and analyze BMDExpress output files. Through two case studies, we demonstrate the capabilities of graphically examining transcriptomic dose-response datasets in a risk assessment context by comparing and observing trends in transcriptomic benchmark doses (BMDs) for biological processes and pathways. Our results illustrate that BMDExpress Data Viewer is a useful tool to visualize, explore and analyze BMDExpress output files. C1 [Kuo, Byron; Webster, A. Francina; Yauk, Carole L.] Hlth Canada, Environm Hlth Sci & Res Bur, Ottawa, ON K1A 0K9, Canada. [Webster, A. Francina] Carleton Univ, Dept Biol, 1125 Colonel By Dr, Ottawa, ON K1S 5B6, Canada. [Thomas, Russell S.] US EPA, Off Res & Dev, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. RP Yauk, CL (reprint author), Hlth Canada, Environm Hlth Sci & Res Bur, Tunneys Pasture, Ottawa, ON K1A 0K9, Canada. EM Carole.Yauk@hc-sc.gc.ca FU Health Canada Genomics Research and Development Initiative; Chemicals Management Plan; Natural Sciences and Engineering Research Council; Ontario Graduate Scholarship FX We thank Jill Franzosa, Reza Farmahin, Nikolai Chepelev, John Wills and Sarah Labib for providing useful comments and suggestions. We thank Reza Farmahin for identifying the MDMB datasets to be included in the analysis. We thank Andrew Williams for providing useful advice on the statistical methods. We acknowledge Scott Auerbach of the National Toxicology Program along with Ruchir Shah, Dan Svoboda and Jason Phillips of Sciome LLC for technical support. This research was funded by the Health Canada Genomics Research and Development Initiative and the Chemicals Management Plan. AFW was supported by the Natural Sciences and Engineering Research Council and the Ontario Graduate Scholarship. NR 29 TC 1 Z9 1 U1 7 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD AUG PY 2016 VL 36 IS 8 BP 1048 EP 1059 DI 10.1002/jat.3265 PG 12 WC Toxicology SC Toxicology GA DP5XA UT WOS:000378570300009 PM 26671443 ER PT J AU Jacobson, MH Barr, DB Marcus, M Muir, AB Lyles, RH Howards, PP Pardo, L Darrow, LA AF Jacobson, Melanie H. Barr, Dana B. Marcus, Michele Muir, Andrew B. Lyles, Robert H. Howards, Penelope P. Pardo, Larissa Darrow, Lyndsey A. TI Serum polybrominated diphenyl ether concentrations and thyroid function in young children SO ENVIRONMENTAL RESEARCH LA English DT Article DE Brominated flame retardants; PBDEs; Thyroid hormones; Thyroid function; Endocrine disruption ID BROMINATED FLAME RETARDANTS; POLYCHLORINATED-BIPHENYLS; HORMONE DISRUPTION; HOUSE-DUST; IN-VITRO; DETECTION LIMITS; EXPOSURE; PBDES; METABOLISM; BIOTRANSFORMATION AB Thyroid hormones are essential for proper neurodevelopment in early life. There is evidence that exposure to polybrominated diphenyl ethers (PBDEs) affects thyroid function, but previous studies have been inconsistent, and no studies among children have been conducted in the United States where PBDE levels are particularly high. Serum levels of seven PBDE congeners and thyroid hormones and other thyroid parameters were measured in 80 children aged 1-5 years from the southeastern United States between 2011 and 2012. Parents of the children completed questionnaires with details on demographics and behaviors. Multivariate linear regression models were used to estimate the associations between serum PBDE levels, expressed as quartiles and as log-transformed continuous variables, and markers of thyroid function. BDE-47, 99, 100 and 153 were detected in > 60% of samples, and were summed (Sigma PBDE). PBDE congeners and Sigma PBDE were positively associated with thyroid-stimulating hormone (TSH). A log-unit increase in IPBDE was associated with a 22.1% increase in TSH (95% CI: 2.0%, 47.7%). Compared with children in the lowest quartile of Sigma PBDE exposure, children in higher quartiles had greater TSH concentrations as modeled on the log-scale (second quartile: (beta=0.32, 95% confidence interval (CD: 0.09, 0.74; third quartile: beta=0.44, 95% CI: 0.04, 0.85; and fourth quartile: (beta=0.49, 95% CI: 0.09, 0.89). There was also a tendency toward lower total T-4 and higher free T-3 with increasing PBDE exposure. Results suggest that exposure to PBDEs during childhood subclinically disrupts thyroid hormone function, with impacts in the direction of hypothyroidism. (c) 2016 Elsevier Inc. All rights reserved. C1 [Jacobson, Melanie H.; Marcus, Michele; Howards, Penelope P.; Darrow, Lyndsey A.] Emory Univ, Rollins Sch Publ Hlth, Dept Epidemiol, Atlanta, GA 30322 USA. [Barr, Dana B.; Marcus, Michele; Darrow, Lyndsey A.] Emory Univ, Rollins Sch Publ Hlth, Dept Environm Hlth, Atlanta, GA 30322 USA. [Muir, Andrew B.] Emory Univ, Sch Med, Dept Pediat, Div Endocrinol, Atlanta, GA 30322 USA. [Lyles, Robert H.] Emory Univ, Rollins Sch Publ Hlth, Dept Biostat & Bioinformat, Atlanta, GA 30322 USA. [Pardo, Larissa] US EPA, Natl Ctr Environm Assessment, Washington, DC 20004 USA. [Darrow, Lyndsey A.] Univ Nevada, Sch Community Hlth Sci, Reno, NV 89557 USA. RP Jacobson, MH (reprint author), 1518 Clifton Rd NE,CNR 3rd Floor, Atlanta, GA 30322 USA. EM mhymanjacobson@emory.edu; dbbarr@emory.edu; mmarcus@emory.edu; abmuir@emory.edu; rlyles@emory.edu; phoward@emory.edu; larissapardo2@gmail.com; ldarrow@emory.edu FU NIEHS [R21ES019697]; NICHD Reproductive, Perinatal, & Pediatric Training Grant [T32HD052460]; NIOSH Environmental and Occupational Epidemiology Training Grant [5T03OH008609-10]; Yerkes National Primate Research Center Base Grant [2P51RR000165-51] FX Funding provided by NIEHS Grant R21ES019697, NICHD Reproductive, Perinatal, & Pediatric Training Grant T32HD052460, and NIOSH Environmental and Occupational Epidemiology Training Grant 5T03OH008609-10.; Assay services were provided by the Biomarkers Core Laboratory at the Yerkes National Primate Research Center. This facility is supported by the Yerkes National Primate Research Center Base Grant 2P51RR000165-51. NR 77 TC 1 Z9 1 U1 6 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0013-9351 EI 1096-0953 J9 ENVIRON RES JI Environ. Res. PD AUG PY 2016 VL 149 BP 222 EP 230 DI 10.1016/j.envres.2016.05.022 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DP3AY UT WOS:000378366000025 PM 27228485 ER PT J AU Shah, I Liu, J Judson, RS Thomas, RS Patlewicz, G AF Shah, Imran Liu, Jie Judson, Richard S. Thomas, Russell S. Patlewicz, Grace TI Systematically evaluating read-across prediction and performance using a local validity approach characterized by chemical structure and bioactivity information SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Article DE Read-across; Nearest neighbors; Local validity domains; (Q)SAR; KNN; Bioactivity; ToxCast ID APPLICABILITY DOMAIN; TIMES-SS; FRAMEWORK; TOOL; UNCERTAINTY; CHALLENGE; TOXICITY; STRATEGY; PROGRAM; MODELS AB Read-across is a popular data gap filling technique within category and analogue approaches for regulatory purposes. Acceptance of read-across remains an ongoing challenge with several efforts underway for identifying and addressing uncertainties. Here we demonstrate an algorithmic, automated approach to evaluate the utility of using in vitro bioactivity data ("bioactivity descriptors", from EPA's ToxCast program) in conjunction with chemical descriptor information to derive local validity domains (specific sets of nearest neighbors) to facilitate read-across for up to ten in vivo repeated dose toxicity study types. Over 3239 different chemical structure descriptors were generated for a set of 1778 chemicals and supplemented with the outcomes from 821 in vitro assays. The read-across prediction of toxicity for 600 chemicals with in vivo data was based on the similarity weighted endpoint outcomes of its nearest neighbors. The approach enabled a performance baseline for read-across predictions of specific study outcomes to be established. Bioactivity descriptors were often found to be more predictive of in vivo toxicity outcomes than chemical descriptors or a combination of both. This generalized read-across (GenRA) forms a first step in systemizing read-across predictions and serves as a useful component of a screening level hazard assessment for new untested chemicals. (C) 2016 Published by Elsevier Inc. C1 [Shah, Imran; Judson, Richard S.; Thomas, Russell S.; Patlewicz, Grace] US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Liu, Jie] Univ Arkansas, Dept Informat Sci, Little Rock, AR 72204 USA. [Liu, Jie] US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Shah, I (reprint author), US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Res Triangle Pk, NC 27711 USA. EM shah.imran@epa.gov OI Judson, Richard/0000-0002-2348-9633 FU appointment to the Research Participation Program at the Office of Research and Development, U.S. Environmental Protection Agency FX This work was supported in part by an appointment to the Research Participation Program at the Office of Research and Development, U.S. Environmental Protection Agency, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. NR 36 TC 0 Z9 0 U1 2 U2 11 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0273-2300 EI 1096-0295 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD AUG PY 2016 VL 79 BP 12 EP 24 DI 10.1016/j.yrtph.2016.05.008 PG 13 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA DO5TB UT WOS:000377844600002 PM 27174420 ER PT J AU Joshi, SS Barnett, B Doerrer, NG Glenn, K Herman, RA Herouet-Guicheney, C Hunst, P Kough, J Ladics, GS McClain, S Papineni, S Poulsen, LK Rascle, JB Tao, AL van Ree, R Ward, J Bowman, CC AF Joshi, Saurabh S. Barnett, Brian Doerrer, Nancy G. Glenn, Kevin Herman, Rod A. Herouet-Guicheney, Corinne Hunst, Penny Kough, John Ladics, Gregory S. McClain, Scott Papineni, Sabitha Poulsen, Lars K. Rascle, Jean-Baptiste Tao, Ai-Lin van Ree, Ronald Ward, Jason Bowman, Christal C. TI Assessment of potential adjuvanticity of Cry proteins SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Editorial Material DE Genetic modification; GM crops; Food/feed safety; Cry proteins; Adjuvant; Bacillus thuringiensis ID THURINGIENSIS-BASED INSECTICIDES; BACILLUS-THURINGIENSIS; IMMUNE-RESPONSES; ALUMINUM-HYDROXIDE; ANTIBODY-RESPONSES; MUCOSAL ADJUVANTS; TRANSGENIC MAIZE; ORAL ADJUVANTS; CHOLERA-TOXIN; MICE AB Genetically modified (GM) crops have achieved success in the marketplace and their benefits extend beyond the overall increase in harvest yields to include lowered use of insecticides and decreased carbon dioxide emissions. The most widely grown GM crops contain geneis for targeted insect protection, herbicide tolerance, or both. Plant expression of Bacillus thuringiensis (Bt) crystal (Cry) insecticidal proteins have been the primary way to impart insect resistance in GM crops. Although deemed safe by regulatory agencies globally, previous studies have been the basis for discussions around the potential immuno-adjuvant effects of Cry proteins. These studies had limitations in study design. The studies used animal models with extremely high doses of Cry proteins, which when given using the ig route were co-administered with an adjuvant. Although the presumption exists that Cry proteins may have immunostimulatory activity and therefore an adjuvanticity risk, the evidence shows that Cry proteins are expressed at very low levels in GM crops and are unlikely to function as adjuvants. This conclusion is based on critical review of the published literature on the effects of immunomodulation by Cry proteins, the history of safe use of Cry proteins in foods, safety of the Bt donor organisms, and pre-market weight of-evidence-based safety assessments for GM crops. (C) 2016 Elsevier Inc. All rights reserved. C1 [Joshi, Saurabh S.; Glenn, Kevin; Ward, Jason] Monsanto Co, 800 North Lindbergh Blvd, St Louis, MO 63167 USA. [Barnett, Brian] BASF Plant Sci, 26 Davis Dr, Res Triangle Pk, NC 27709 USA. [Doerrer, Nancy G.] ILSI Hlth & Environm Sci Inst, 1156 Fifteenth St,NW Suite 200, Washington, DC 20005 USA. [Herman, Rod A.; Papineni, Sabitha] Dow AgroSci, 9330 Zionsville Rd, Indianapolis, IN 46268 USA. [Herouet-Guicheney, Corinne; Rascle, Jean-Baptiste] Bayer CropSci, Bayer SAS, 355 Rue Dostoievski, F-06903 Sophia Antipolis, France. [Hunst, Penny; Bowman, Christal C.] Bayer CropSci, 2 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Kough, John] US EPA, Off Pesticide Programs, Ariel Rios Bldg,MC 7511R,1200 Penn Ave,NW, Washington, DC 20460 USA. [Ladics, Gregory S.] DuPont Haskell Global Centers Hlth & Environm Sci, 1090 Elkton Rd, Newark, DE 19711 USA. [McClain, Scott] Syngenta Crop Protect LLC, 3054 E Cornwallis Rd, Res Triangle Pk, NC 27709 USA. [Poulsen, Lars K.] Univ Copenhagen, Gentofte Hosp, Allergy Clin, Niels Andersens Vej 65,Dept 22,1st Floor, DK-2900 Hellerup, Denmark. [Tao, Ai-Lin] Guangzhou Med Univ, 250 Changgang Rd East, Guangzhou 510260, Guangdong, Peoples R China. [van Ree, Ronald] Univ Amsterdam, Acad Med Ctr, Dept Expt Immunol, Meibergdreef 9,Room KO-130, NL-1105 AZ Amsterdam, Netherlands. [van Ree, Ronald] Univ Amsterdam, Acad Med Ctr, Dept Otorhinolaryngol, Meibergdreef 9,Room KO-130, NL-1105 AZ Amsterdam, Netherlands. RP Bowman, CC (reprint author), Bayer CropSci, 2 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM saurabh.joshi@monsanto.com; brian.barnett@basf.com; ndoerrer@hesiglobal.org; kevin.c.glenn@monsanto.com; raherman@dow.com; corinne.herouet-guicheney@bayer.com; penny.hunst@bayercropscience.com; kough.john@epa.gov; gregory.s.ladics@dupont.com; scott.mcclain@syngenta.com; spapineni@dow.com; lkpallgy@mail.dk; jean-baptiste.rascle@bayer.com; AerobiologiaTao@163.com; r.vanree@amc.uva.nl; jason.m.ward@monsanto.com; christal.bowman@bayer.com NR 102 TC 0 Z9 0 U1 12 U2 29 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0273-2300 EI 1096-0295 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD AUG PY 2016 VL 79 BP 149 EP 155 DI 10.1016/j.yrtph.2016.04.005 PG 7 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA DO5TB UT WOS:000377844600021 PM 27105772 ER PT J AU Carbone, F Landis, MS Gencarelli, CN Naccarato, A Sprovieri, F De Simone, F Hedgecock, IM Pirrone, N AF Carbone, F. Landis, M. S. Gencarelli, C. N. Naccarato, A. Sprovieri, F. De Simone, F. Hedgecock, I. M. Pirrone, N. TI Sea surface temperature variation linked to elemental mercury concentrations measured on Mauna Loa SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article DE mercury; sea surface temperature; Mauna Loa Observatory; ocean atmosphere exchange ID EMPIRICAL MODE DECOMPOSITION; GASEOUS MERCURY; TIME-SERIES; CONSTRAINTS; ATMOSPHERE; DEPOSITION; CYCLE; FIELD; AIR AB The Hg-0 time series recorded at the Mauna Loa Observatory (MLO) in Hawaii between 2002 and 2009 has been analyzed using Empirical Mode Decomposition. This technique has been used in numerous contexts in order to identify periodical variations in time series data. The periodicities observed in the tropical Pacific sea surface temperature (SST), through the data collected from five buoys, are also observed in Hg-0 concentrations and the relative humidity measured at the MLO. The lag times in the observed periodicities are related to the position of the buoys with respect to the measurement site. This demonstrates a direct link between climatological phenomena, in this case SST, and measured Hg-0 and reflects the influence of ocean SST on Hg-0 evasion. This is the first long-term experimental evidence of such a direct effect on Hg-0 evasion from the oceanic surface driven by temperature. C1 [Carbone, F.; Gencarelli, C. N.; Naccarato, A.; Sprovieri, F.; De Simone, F.; Hedgecock, I. M.] UNICAL Polifunz, Div Rende, CNR Inst Atmospher Pollut Res, Arcavacata Di Rende, Italy. [Landis, M. S.] US EPA, Off Res & Dev, Durham, NC USA. [Pirrone, N.] Area Ric Roma 1, CNR Inst Atmospher Pollut Res, Rome, Italy. RP Carbone, F (reprint author), UNICAL Polifunz, Div Rende, CNR Inst Atmospher Pollut Res, Arcavacata Di Rende, Italy. EM f.carbone@iia.cnr.it RI Naccarato, Attilio/M-4141-2016; OI Naccarato, Attilio/0000-0001-5799-5369; Hedgecock, Ian/0000-0002-3743-1870 FU EPA through its Office of Research and Development and Office of International and Tribal Affairs; EU GMOS project [FP7-265113] FX The EPA through its Office of Research and Development and Office of International and Tribal Affairs partially funded and contributed to this research. The views expressed in this paper are those of the authors and do not necessarily reflect the views or policies of EPA. It has been subjected to Agency review and approved for publication. Mention of trade names or commercial products does not constitute an endorsement or recommendation for use. We thank Ram Vedantham, Sania Tong-Argao, and Carry Croghan (EPA ORD) for their assistance with data Quality Assurance; and Russell Schell, Aidan Colton, Daryl Kuniyuki, Alan Yoshinaga, and Poai Suganuma (NOAA) for MLO site logistical support. The authors from CNR-IIA gratefully acknowledge funding through the EU GMOS project (FP7-265113). The Hg0 data set is available from the author on request (landis.matthew@epa.gov). The buoy data can be obtained from the TAO/TRITON database at http://www.pmel.noaa.gov/tao/index. shtml and from the National Buoy Data Center database http://www.ndbc.noaa.gov/. NR 38 TC 3 Z9 3 U1 5 U2 5 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 EI 1944-8007 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD JUL 28 PY 2016 VL 43 IS 14 BP 7751 EP 7757 DI 10.1002/2016GL069252 PG 7 WC Geosciences, Multidisciplinary SC Geology GA DV9VL UT WOS:000383290200057 ER PT J AU Dias, E Morais, P Cotter, AM Antunes, C Hoffman, JC AF Dias, Ester Morais, Pedro Cotter, Anne M. Antunes, Carlos Hoffman, Joel C. TI Estuarine consumers utilize marine, estuarine and terrestrial organic matter and provide connectivity among these food webs SO MARINE ECOLOGY PROGRESS SERIES LA English DT Article DE Allochthonous organic matter; River discharge; Pelagic; Benthic; Stable isotopes; Extreme weather events ID STABLE-ISOTOPE ANALYSIS; YORK RIVER ESTUARY; SAN-FRANCISCO BAY; SECONDARY PRODUCTION; NITROGEN ISOTOPE; FRESH-WATER; NUTRIENT ENRICHMENT; ZEBRA MUSSELS; CARBON; VIRGINIA AB The flux of organic matter (OM) across ecosystem boundaries can influence estuarine food web dynamics and productivity. However, this process is seldom investigated taking into account all the adjacent ecosystems (e.g. ocean, river, land) and different hydrological settings (i.e. river discharge). Therefore, we aimed to quantify the contribution of autochthonous and allochthonous OM to the lower food web along the estuarine salinity gradient, under different river discharge conditions. The carbon (C) and nitrogen (N) stable isotope ratios of pelagic (zooplankton) and benthic (Corbicula fluminea) primary consumers indicated that they rely on a mixture of autochthonous and allochthonous OM, including terrestrial-derived OM. Unexpectedly, the highest contribution of terrestrial-derived OM to the estuarine food web was observed during a low river discharge period (up to 70%), that succeeded a large winter flood, showing that extreme weather events may produce prolonged effects on estuarine food webs. The contribution of marine-derived OM was higher during low river discharge periods (up to 88%) and was restricted to the seaward end of the estuary. Concomitantly, the contribution of phytoplankton to primary consumers was the highest observed (up to 91%). Further, both pelagic and benthic consumers also relied on benthic C (i.e. sediment OM and microphytobenthos). This study demonstrates that primary consumers enhance connectivity between estuarine ecosystems by utilizing subsidies of terrestrial and marine origin, and also between estuarine habitats through reliance on pelagic and benthic OM. C1 [Dias, Ester] Univ Porto, ICBAS, Rua Jorge Viterbo Ferreira 228, P-4050313 Oporto, Portugal. [Dias, Ester; Morais, Pedro; Antunes, Carlos] Univ Porto, CIMAR CIIMAR Ctr Interdisciplinar Invest Marinha, Rua Bragas 289, P-4050123 Oporto, Portugal. [Morais, Pedro] Univ Algarve, CCMAR Ctr Marine Sci, Campus Gambelas, P-8005139 Faro, Portugal. [Cotter, Anne M.; Hoffman, Joel C.] US Environm Protect Agcy, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Antunes, Carlos] Aquamuseu Rio Minho, Parque Castelinho S-N, P-4920290 Vila Nova De Cerveira, Portugal. RP Dias, E (reprint author), Univ Porto, ICBAS, Rua Jorge Viterbo Ferreira 228, P-4050313 Oporto, Portugal.; Dias, E (reprint author), Univ Porto, CIMAR CIIMAR Ctr Interdisciplinar Invest Marinha, Rua Bragas 289, P-4050123 Oporto, Portugal. EM esterdias@ciimar.up.pt RI Morais, Pedro/F-2815-2011; OI Morais, Pedro/0000-0002-0861-5566; Antunes, Carlos/0000-0003-3736-5743 FU Foundation for Science and Technology (FCT); European Regional Development Fund (ERDF); Strategic Funding through national funds provided by FCT [UID/Multi/04423/2013]; framework of the programme PT FX We thank Katie Bentley, Jacinto Cunha, Martina Ilarri and Luis Vieira for helping with POM and zooplankton samples; Rute Pinto for providing the map of the study area; and 3 anonymous reviewers for their helpful comments. E.D. (SFRH/BPD/104019/2014) and P.M. (SFRH/BPD/40832/2007, INCENTIVO/MAR/UI0350/2014) were supported by post-doc scholarships financed by the Foundation for Science and Technology (FCT). This work was partially supported by the Strategic Funding UID/Multi/04423/2013 through national funds provided by FCT and European Regional Development Fund (ERDF), in the framework of the programme PT2020. The views expressed in this paper are those of the authors and do not necessarily reflect the views or policies of the US EPA. NR 60 TC 1 Z9 1 U1 13 U2 13 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0171-8630 EI 1616-1599 J9 MAR ECOL PROG SER JI Mar. Ecol.-Prog. Ser. PD JUL 28 PY 2016 VL 554 BP 21 EP 34 DI 10.3354/meps11794 PG 14 WC Ecology; Marine & Freshwater Biology; Oceanography SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography GA DW6ZW UT WOS:000383801200002 ER PT J AU Gallagher, JE Wilkie, AA Cordner, A Hudgens, EE Ghio, AJ Birch, RJ Wade, TJ AF Gallagher, Jane E. Wilkie, Adrien A. Cordner, Alissa Hudgens, Edward E. Ghio, Andrew J. Birch, Rebecca J. Wade, Timothy J. TI Factors associated with self-reported health: implications for screening level community-based health and environmental studies SO BMC PUBLIC HEALTH LA English DT Article DE Self-reported health; Screening level health assessment; Clinical measures; Metal mixtures analyses; NHANES ID CELL DISTRIBUTION WIDTH; RATED HEALTH; LIFE-STYLE; SOCIOECONOMIC-STATUS; CUMULATIVE RISK; GENERAL HEALTH; US ADULTS; MORTALITY; INDEX; DETERMINANTS AB Background: Advocates for environmental justice, local, state, and national public health officials, exposure scientists, need broad-based health indices to identify vulnerable communities. Longitudinal studies show that perception of current health status predicts subsequent mortality, suggesting that self-reported health (SRH) may be useful in screening-level community assessments. This paper evaluates whether SRH is an appropriate surrogate indicator of health status by evaluating relationships between SRH and sociodemographic, lifestyle, and health care factors as well as serological indicators of nutrition, health risk, and environmental exposures. Methods: Data were combined from the 2003-2006 National Health and Nutrition Examination Surveys for 1372 nonsmoking 20-50 year olds. Ordinal and binary logistic regression was used to estimate odds ratios and 95 % confidence intervals of reporting poorer health based on measures of nutrition, health condition, environmental contaminants, and sociodemographic, health care, and lifestyle factors. Results: Poorer SRH was associated with several serological measures of nutrition, health condition, and biomarkers of toluene, cadmium, lead, and mercury exposure. Race/ethnicity, income, education, access to health care, food security, exercise, poor mental and physical health, prescription drug use, and multiple health outcome measures (e.g., diabetes, thyroid problems, asthma) were also associated with poorer SRH. Conclusion: Based on the many significant associations between SRH and serological assays of health risk, sociodemographic measures, health care access and utilization, and lifestyle factors, SRH appears to be a useful health indicator with potential relevance for screening level community-based health and environmental studies. C1 [Gallagher, Jane E.; Hudgens, Edward E.; Ghio, Andrew J.; Wade, Timothy J.] US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Mail Drop 58C, Res Triangle Pk, NC 27711 USA. [Wilkie, Adrien A.] US EPA, Oak Ridge Inst Sci & Educ, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Mail Drop 58C, Res Triangle Pk, NC 27711 USA. [Cordner, Alissa] Whitman Coll, Dept Sociol, 345 Boyer Ave, Walla Walla, WA 99362 USA. [Birch, Rebecca J.] Westat Corp, 1600 Res Blvd, Rockville, MD 20850 USA. RP Wade, TJ (reprint author), US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Mail Drop 58C, Res Triangle Pk, NC 27711 USA. EM Wade.Tim@epa.gov FU National Health and Environmental Effects Research Laboratory [EP-D-12-050]; National Center for Computational Toxicology within U.S. Environmental Protection Agency (EPA) Office of Research and Development FX This study was funded by the National Health and Environmental Effects Research Laboratory (contract number EP-D-12-050) and the National Center for Computational Toxicology within the U.S. Environmental Protection Agency (EPA) Office of Research and Development. This project was supported in part by an appointment to the Research Participation Program at the Office of Research and Development, U.S. EPA, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. NR 50 TC 0 Z9 0 U1 8 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2458 J9 BMC PUBLIC HEALTH JI BMC Public Health PD JUL 26 PY 2016 VL 16 AR 640 DI 10.1186/s12889-016-3321-5 PG 15 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DR9QI UT WOS:000380230800007 PM 27460934 ER PT J AU Warren, JL Stingone, JA Herring, AH Luben, TJ Fuentes, M Aylsworth, AS Langlois, PH Botto, LD Correa, A Olshan, AF AF Warren, Joshua L. Stingone, Jeanette A. Herring, Amy H. Luben, Thomas J. Fuentes, Montserrat Aylsworth, Arthur S. Langlois, Peter H. Botto, Lorenzo D. Correa, Adolfo Olshan, Andrew F. CA Natl Birth Defects Prevention TI Bayesian multinomial probit modeling of daily windows of susceptibility for maternal PM2.5 exposure and congenital heart defects SO STATISTICS IN MEDICINE LA English DT Article DE air pollution; Bayesian modeling; birth defects; critical windows; Gaussian process ID AMBIENT AIR-POLLUTION; BIRTH-DEFECTS; PREVALENCE; METAANALYSIS; PREVENTION; ANOMALIES; RISK AB Epidemiologic studies suggest that maternal ambient air pollution exposure during critical periods of pregnancy is associated with adverse effects on fetal development. In this work, we introduce new methodology for identifying critical periods of development during post-conception gestational weeks 2-8 where elevated exposure to particulate matter less than 2.5 mu m (PM2.5) adversely impacts development of the heart. Past studies have focused on highly aggregated temporal levels of exposure during the pregnancy and have failed to account for anatomical similarities between the considered congenital heart defects. We introduce a multinomial probit model in the Bayesian setting that allows for joint identification of susceptible daily periods during pregnancy for 12 types of congenital heart defects with respect to maternal PM2.5 exposure. We apply the model to a dataset of mothers from the National Birth Defect Prevention Study where daily PM2.5 exposures from post-conception gestational weeks 2-8 are assigned using predictions from the downscaler pollution model. This approach is compared with two aggregated exposure models that define exposure as the average value over post-conception gestational weeks 2-8 and the average over individual weeks, respectively. Results suggest an association between increased PM2.5 exposure on post-conception gestational day 53 with the development of pulmonary valve stenosis and exposures during days 50 and 51 with tetralogy of Fallot. Significant associations are masked when using the aggregated exposure models. Simulation study results suggest that the findings are robust to multiple sources of error. The general form of the model allows for different exposures and health outcomes to be considered in future applications. Copyright (c) 2016 John Wiley & Sons, Ltd. C1 [Warren, Joshua L.] Yale Univ, Sch Publ Hlth, Dept Biostat, POB 208034, New Haven, CT 06520 USA. [Stingone, Jeanette A.] Icahn Sch Med Mt Sinai, Dept Prevent Med, New York, NY 10029 USA. [Herring, Amy H.] UNC Gillings Sch Global Publ Hlth, Dept Biostat, Chapel Hill, NC USA. [Luben, Thomas J.] USA Environm Protect Agcy, Natl Ctr Environm Assessment, Off Res & Dev, Res Triangle Pk, NC USA. [Fuentes, Montserrat] N Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. [Aylsworth, Arthur S.] Univ N Carolina, Dept Pediat & Genet, Chapel Hill, NC USA. [Langlois, Peter H.] Texas Dept State Hlth Serv, Texas Ctr Birth Defects Res & Prevent, Austin, TX USA. [Botto, Lorenzo D.] Univ Utah, Dept Pediat, Div Med Genet, Salt Lake City, UT USA. [Correa, Adolfo] Univ Mississippi, Med Ctr, Dept Pediat, Jackson, MS 39216 USA. [Olshan, Andrew F.] UNC Gillings Sch Global Publ Hlth, Dept Epidemiol, Chapel Hill, NC USA. RP Warren, JL (reprint author), Yale Univ, Sch Publ Hlth, Dept Biostat, POB 208034, New Haven, CT 06520 USA. EM joshua.warren@yale.edu FU Centers for Disease Control and Prevention [02081, U50CCU422096]; National Institute of Environmental Health Sciences [5R01ES014843-02, R01ES020619, T32ES007018] FX This study was supported in part through cooperative agreements under Program Announcement 02081 from the Centers for Disease Control and Prevention to the centers participating in the National Birth Defects Prevention Study including cooperative agreement U50CCU422096. Additional support was provided by the National Institute of Environmental Health Sciences (Fuentes, 5R01ES014843-02; Herring, R01ES020619; and Herring, T32ES007018). NR 28 TC 0 Z9 2 U1 5 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0277-6715 EI 1097-0258 J9 STAT MED JI Stat. Med. PD JUL 20 PY 2016 VL 35 IS 16 BP 2786 EP 2801 DI 10.1002/sim.6891 PG 16 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA DP5PJ UT WOS:000378549200008 PM 26853919 ER PT J AU Xing, J Wang, JD Mathur, R Pleim, J Wang, SX Hogrefe, C Gan, CM Wong, DC Hao, JM AF Xing, Jia Wang, Jiandong Mathur, Rohit Pleim, Jonathan Wang, Shuxiao Hogrefe, Christian Gan, Chuen-Meei Wong, David C. Hao, Jiming TI Unexpected Benefits of Reducing Aerosol Cooling Effects SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID WRF-CMAQ MODEL; AIR-POLLUTION; GLOBAL BURDEN; UNITED-STATES; NORTHERN-HEMISPHERE; SYSTEMATIC ANALYSIS; BLACK CARBON; HUMAN HEALTH; MORTALITY; CHINA AB Impacts of aerosol cooling are not limited to changes in surface temperature since modulation of atmospheric dynamics resulting from the increased stability can deteriorate local air quality and impact human health. Health impacts from two manifestations of the aerosol direct effects (ADE) are estimated in this study: (1) the effect on surface temperature and (2) the effect on air quality through atmospheric dynamics. Average mortalities arising from the enhancenient of surface PM2.5 concentration due to ADE in East Asia, North America and Europe are estimated to be 3-6 times higher than reduced mortality from decreases of temperature due to ADE. Our results suggest that mitigating aerosol pollution is beneficial in decreasing the impacts of climate change arising from these two mani-festations of ADE health impacts. Thus, decreasing aerosol pollution gets direct benefits on hcalth-, and indirect benefits on health through changes in local climate and not offsetting changes associated only with temperature modulations as traditionally thought. The modulation of air pollution due to ADE also translates into an additional human health dividend in regions (e.g., U.S. Europe) with air pollution control measures but a penalty for regions (e.g., Asia) witnessing rapid deterioration in air quality. C1 [Xing, Jia; Wang, Jiandong; Wang, Shuxiao; Hao, Jiming] Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China. [Xing, Jia; Wang, Jiandong; Mathur, Rohit; Pleim, Jonathan; Hogrefe, Christian; Gan, Chuen-Meei; Wong, David C.] US EPA, Res Triangle Pk, NC 27711 USA. RP Wang, SX (reprint author), Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China.; Mathur, R (reprint author), US EPA, Res Triangle Pk, NC 27711 USA. EM mathur.rohit@epa.gov; shxwang@tsinghua.edu.cn RI wang, shuxiao/H-5990-2011; Pleim, Jonathan Pleim/C-1331-2017 OI wang, shuxiao/0000-0001-9727-1963; Pleim, Jonathan Pleim/0000-0001-6190-6082 FU Department of energy [DE-SC000378]; EPA [RW-89-9233260 1]; MEP's Special Funds for Research on Public Welfare [201409002]; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB05020300]; National Research Council Research Associateship Award at U.S. EPA; China Scholar Council Award at U.S. EPA FX Although this work has been reviewed and approved for publication by the U.S. Environmental Protection Agency (EPA), it does not reflect the views and policies of the agency. Mention of trade names or commercial products does not constitute endorsement. We thank Terry Keating, Bryan Hubbell, Chris Nolte, Jason Sacks, Neal Fann (EPA) and the two anonymous reviewers for helpful suggestions on initial versions of this manuscript. This work was supported in part by an interagency agreement between the Department of energy project (IA number is DE-SC000378) and EPA (IA number is RW-89-9233260 1), also the MEP's Special Funds for Research on Public Welfare (201409002) and Strategic Priority Research Program of the Chinese Academy of Sciences (XDB05020300). This research was performed while J.X. and C.-M.G. held a National Research Council Research Associateship Award at U.S. EPA, and J.W. held a China Scholar Council Award at U.S. EPA. The authors gratefully acknowledge the availability of population data from GPW and cause of death data from HEI. NR 42 TC 0 Z9 0 U1 6 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 19 PY 2016 VL 50 IS 14 BP 7527 EP 7534 DI 10.1021/acs.est.6b00767 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DS0OD UT WOS:000380295700029 PM 27310144 ER PT J AU Peters, TM Sawvel, EJ Willis, R West, RR Casuccio, GS AF Peters, Thomas M. Sawvel, Eric J. Willis, Robert West, Roger R. Casuccio, Gary S. TI Performance of Passive Samplers Analyzed by Computer -Controlled Scanning Electron Microscopy to Measure PM10-2.5 SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID COARSE PARTICULATE MATTER; AEROSOL SAMPLER; SPATIAL VARIABILITY; CLEVELAND; PARTICLES; CAPTURE; AREA; OHIO AB We report on the precision and accuracy of measuring PM10-2.5 and its components with particles collected by passive, aerosol samplers and analyzed by computer controlled scanning electron microscopy with energy dispersive X-ray spectroscopy. Passive samplers were deployed for week-long intervals in triplicate and colocated with a federal reference method sampler at three sites and for 5 weeks in summer 2009 and 5 weeks in winter 2010 in Cleveland, OH. The limit of detection of the passive method for PM10-2.5 determined from blank analysis was 2.8 mu g m(-3). Overall precision expressed as root-mean-square coefficient of variation (CVRMS) improved with increasing concentrations (37% for all samples, n = 30; 19% for PM10-2.5 > 10 mu g m(-3)) n = 9; and 10% for PM10-2.5 > 15 mu g m(-3), n = 4). The linear regression of PM10-2.5 measured passively on that measured with the reference sampler exhibited an intercept not statistically different than zero (p = 0.46) and a slope not statistically different from unity (p = 0.92). Triplicates with high CVs (CV > 40%, n = 5) were attributed to low particle counts (and mass concentrations), spurious counts attributed to salt particles, and Al-rich particles. This work provides important quantitative observations that can help guide future development and use of passive samplers for measuring atmospheric particulate matter. C1 [Peters, Thomas M.; Sawvel, Eric J.] Univ Iowa, Iowa City, IA 52242 USA. [Willis, Robert] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27709 USA. [West, Roger R.; Casuccio, Gary S.] RJ Lee Grp Inc, Monroeville, PA 15146 USA. RP Peters, TM (reprint author), Univ Iowa, Iowa City, IA 52242 USA. EM thomas-m-peters@uiowa.edu FU United States Environmental Protection Agency through its Office of Research and Development [EP09D000463, EP11D000010] FX We thank the numerous support staff of EPA Region 5, Cleveland Department of Air Quality, and Alion Science and Technology that were involved in deployment and retrieval of passive samplers. The United States Environmental Protection Agency through its Office of Research and Development funded and collaborated in the research described here under contracts EP09D000463 and EP11D000010 to the University of Iowa. It has been subjected to Agency review and approved for publication. NR 17 TC 0 Z9 0 U1 6 U2 6 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 19 PY 2016 VL 50 IS 14 BP 7581 EP 7589 DI 10.1021/acs.est.6b01105 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DS0OD UT WOS:000380295700035 PM 27300163 ER PT J AU Yost, EE Stanek, J DeWoskin, RS Burgoon, LD AF Yost, Erin E. Stanek, John DeWoskin, Robert S. Burgoon, Lyle D. TI Estimating the Potential Toxicity of Chemicals Associated with Hydraulic Fracturing Operations Using Quantitative Structure-Activity Relationship Modeling SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID SHALE GAS DEVELOPMENT; COST-EFFECTIVE USE; MONITORING RESOURCES; WATER; GROUNDWATER; PHARMACEUTICALS; BIODEGRADATION; PROMOTE; QSARS AB The United States Environmental Protection Agency (EPA) identified 1173 chemicals associated with hydraulic fracturing fluids, flowback, or produced water, of which 1026 (87%) lack chronic oral toxicity values for human health assessments. To facilitate the ranking and prioritization of chemicals that lack toxicity values, it may be useful to employ toxicity estimates from quantitative structure activity relationship (QSAR) models. Here we describe an approach for applying the results of a QSAR model from the TOPKAT program suite, which provides estimates of the rat chronic oral lowest-observed-adverse-effect level (LOAEL). Of the 1173 chemicals, TOPKAT was able to generate LOAEL estimates for 515 (44%). To address the uncertainty associated with these estimates, we assigned qualitative confidence scores (high, medium, or low) to each TOPKAT LOAEL estimate, and found 481 to be high-confidence. For 48 both a high-confidence TOPKAT LOAEL estimate and a chronic oral reference dose from EPA's Integrated Risk Information System (IRIS) database, Spearman rank correlation identified 68% agreement between the two values (permutation p-value =1 X 10(-11)). These results provide support for the use of TOPKAT LOAEL estimates in identifying and prioritizing potentially hazardous chemicals. High-confidence TOPKAT LOAEL estimates were available for 389 of 1026 hydraulic fracturing-related chemicals that lack chronic oral RfVs and OSFs from EPA-identified sources, including a subset of chemicals that are frequently used in hydraulic fracturing fluids. C1 [Yost, Erin E.; Stanek, John; DeWoskin, Robert S.; Burgoon, Lyle D.] US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. [DeWoskin, Robert S.] EtioLogic LLC, Durham, NC 27717 USA. [Burgoon, Lyle D.] US Army Engineer Res & Dev Ctr, Environm Lab, Res Triangle Pk, NC 27709 USA. RP Burgoon, LD (reprint author), US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA.; Burgoon, LD (reprint author), US Army Engineer Res & Dev Ctr, Environm Lab, Res Triangle Pk, NC 27709 USA. EM Lyle.D.Burgoon@usace.army.mil OI Burgoon, Lyle/0000-0003-4977-5352 FU U.S. Department of Energy; EPA FX We thank Allen Davis, John Whalen, John Vandenberg, and Steve Dutton for their comments on previous drafts of this manuscript. This work was supported in part by an appointment to the Research Participation Program at the EPA National Center for Environmental Assessment, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. The views expressed in this manuscript are those of the authors and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 35 TC 0 Z9 0 U1 20 U2 26 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 19 PY 2016 VL 50 IS 14 BP 7732 EP 7742 DI 10.1021/acs.est.5b05327 PG 11 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DS0OD UT WOS:000380295700052 PM 27172125 ER PT J AU Winston, JJ Emch, M Meyer, RE Langlois, P Weyer, P Mosley, B Olshan, AF Band, LE Luben, TJ AF Winston, Jennifer J. Emch, Michael Meyer, Robert E. Langlois, Peter Weyer, Peter Mosley, Bridget Olshan, Andrew F. Band, Lawrence E. Luben, Thomas J. CA Natl Birth Defects Prevention Stud TI Hypospadias and maternal exposure to atrazine via drinking water in the National Birth Defects Prevention study SO ENVIRONMENTAL HEALTH LA English DT Article DE Hypospadias; Birth defects; Atrazine; Groundwater; Surface water; Endocrine disruptors ID XENOPUS-LAEVIS; UNITED-STATES; MALFORMATIONS; MODELS; FROGS AB Background: Hypospadias is a relatively common birth defect affecting the male urinary tract. It has been suggested that exposure to endocrine disrupting chemicals might increase the risk of hypospadias by interrupting normal urethral development. Methods: Using data from the National Birth Defects Prevention Study, a population-based case-control study, we considered the role of maternal exposure to atrazine, a widely used herbicide and potential endocrine disruptor, via drinking water in the etiology of 2nd and 3rd degree hypospadias. We used data on 343 hypospadias cases and 1,422 male controls in North Carolina, Arkansas, Iowa, and Texas from 1998-2005. Using catchment level stream and groundwater contaminant models from the US Geological Survey, we estimated atrazine concentrations in public water supplies and in private wells. We assigned case and control mothers to public water supplies based on geocoded maternal address during the critical window of exposure for hypospadias (i.e., gestational weeks 6-16). Using maternal questionnaire data about water consumption and drinking water, we estimated a surrogate for total maternal consumption of atrazine via drinking water. We then included additional maternal covariates, including age, race/ethnicity, parity, and plurality, in logistic regression analyses to consider an association between atrazine and hypospadias. Results: When controlling for maternal characteristics, any association between hypospadias and daily maternal atrazine exposure during the critical window of genitourinary development was found to be weak or null (odds ratio for atrazine in drinking water = 1.00, 95 % CI = 0.97 to 1.03 per 0.04 mu g/day increase; odds ratio for maternal consumption = 1.02, 95 % CI = 0.99 to 1.05; per 0.05 mu g/day increase). Conclusions: While the association that we observed was weak, our results suggest that additional research into a possible association between atrazine and hypospadias occurrence, using a more sensitive exposure metric, would be useful. C1 [Winston, Jennifer J.; Emch, Michael] Univ N Carolina, Carolina Populat Ctr, Chapel Hill, NC 27599 USA. [Emch, Michael; Band, Lawrence E.] Univ N Carolina, Dept Geog, Chapel Hill, NC USA. [Meyer, Robert E.] State Ctr Hlth Stat, North Carolina Birth Defects Monitoring Program, Raleigh, NC USA. [Meyer, Robert E.] Univ N Carolina, Dept Maternal & Child Hlth, Chapel Hill, NC USA. [Langlois, Peter] Texas Dept State Hlth Serv, Birth Defects Epidemiol & Surveillance Branch, Austin, TX USA. [Weyer, Peter] Univ Iowa, Ctr Hlth Effects Environm Contaminat, Iowa City, IA USA. [Mosley, Bridget] Arkansas Childrens Hosp, Dept Pediat, Little Rock, AR 72202 USA. [Olshan, Andrew F.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC USA. [Band, Lawrence E.] Univ N Carolina, Inst Environm, Chapel Hill, NC USA. [Luben, Thomas J.] US EPA, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. RP Winston, JJ (reprint author), Univ N Carolina, Carolina Populat Ctr, Chapel Hill, NC 27599 USA. EM jwinston@unc.edu FU University of North Carolina at Chapel Hill [T32 HD007168, R24 HD050924]; Eunice Kennedy Shriver National Institute of Child Health and Human Development; Centers for Disease Control and Prevention [U50CCU422096] FX This research received support from the Population Research Training grant (T32 HD007168) and the Population Research Infrastructure Program awarded to the Carolina Population Center (R24 HD050924) at The University of North Carolina at Chapel Hill by the Eunice Kennedy Shriver National Institute of Child Health and Human Development. This study was also supported by a cooperative agreement from the Centers for Disease Control and Prevention (U50CCU422096). NR 22 TC 0 Z9 0 U1 4 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1476-069X J9 ENVIRON HEALTH-GLOB JI Environ. Health PD JUL 15 PY 2016 VL 15 AR 76 DI 10.1186/s12940-016-0161-9 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DR6EI UT WOS:000379994500001 PM 27422386 ER PT J AU Belair, DG Schwartz, MP Knudsen, T Murphy, WL AF Belair, David G. Schwartz, Michael P. Knudsen, Thomas Murphy, William L. TI Human iPSC-derived endothelial cell sprouting assay in synthetic hydrogel arrays SO ACTA BIOMATERIALIA LA English DT Article DE Angiogenic sprouting; Chemically-defined assay; Poly(ethylene glycol) hydrogels; Extracellular matrix; Endothelial cells; Thiol-ene chemistry; ToxCast ID GLYCOL) DIACRYLATE HYDROGELS; TYROSINE KINASE INHIBITOR; ANGIOGENESIS IN-VITRO; MATRIX METALLOPROTEINASES; BINDING-SITE; EXTRACELLULAR MATRICES; NETWORK FORMATION; RAPAMYCIN MTOR; GROWTH; PEPTIDE AB Activation of vascular endothelial cells (ECs) by growth factors initiates a cascade of events during angiogenesis in vivo consisting of EC tip cell selection, sprout formation, EC stalk cell proliferation, and ultimately vascular stabilization by support cells. Although EC functional assays can recapitulate one or more aspects of angiogenesis in vitro, they are often limited by undefined substrates and lack of dependence on key angiogenic signaling axes. Here, we designed and characterized a chemically-defined model of endothelial sprouting behavior in vitro using human induced pluripotent stem cell-derived endothelial cells (iPSC-ECs). We rapidly encapsulated iPSC-ECs at high density in poly(ethylene glycol) (PEG) hydro gel spheres using thiol-ene chemistry and subsequently encapsulated cell-dense hydrogel spheres in a cell-free hydrogel layer. The hydrogel sprouting array supported pro-angiogenic phenotype of iPSC-ECs and supported growth factor-dependent proliferation and sprouting behavior. iPSC-ECs in the sprouting model responded appropriately to several reference pharmacological angiogenesis inhibitors of vascular endothelial growth factor, NF-kappa B, matrix metalloproteinase-2/9, protein kinase activity, and 6-tubulin, which confirms their functional role in endothelial sprouting. A blinded screen of 38 putative vascular disrupting compounds from the US Environmental Protection Agency's ToxCast library identified six compounds that inhibited iPSC-EC sprouting and five compounds that were overtly cytotoxic to iPSC-ECs at a single concentration. The chemically-defined iPSC-EC sprouting model (iSM) is thus amenable to enhanced-throughput screening of small molecular libraries for effects on angiogenic sprouting and iPSC-EC toxicity assessment. Statement of Significance Angiogenesis assays that are commonly used for drug screening and toxicity assessment applications typically utilize natural substrates like Matrigel (TM) that are difficult to spatially pattern, costly, ill-defined, and may exhibit lot-to-lot variability. Herein, we describe a novel angiogenic sprouting assay using chemically-defined, bioinert poly(ethylene glycol) hydrogels functionalized with biomimetic peptides to promote cell attachment and degradation in a reproducible format that may mitigate the need for natural substrates. The quantitative assay of angiogenic sprouting here enables precise control over the initial conditions and can be formulated into arrays for screening. The sprouting assay here was dependent on key angiogenic signaling axes in a screen of angiogenesis inhibitors and a blinded screen of putative vascular disrupting compounds from the US-EPA. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. C1 [Belair, David G.; Schwartz, Michael P.; Murphy, William L.] Univ Wisconsin Madison, Dept Biomed Engn, Madison, WI USA. [Knudsen, Thomas] US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Res Triangle Pk, NC USA. [Murphy, William L.] Univ Wisconsin Madison, Mat Sci Program, Madison, WI USA. [Murphy, William L.] Univ Wisconsin Madison, Dept Orthoped & Rehabil, Madison, WI USA. RP Murphy, WL (reprint author), Wisconsin Inst Med Res II, 1111 Highland Ave,Room 5405, Madison, WI 53705 USA. EM wlmurphy@wisc.edu RI Schwartz, Mike/O-7963-2016 OI Schwartz, Mike/0000-0003-3785-6606 FU National Institutes of Health [T32 HL007936-12, RO1HL093282, R21 EB016381]; Environmental Protection Agency STAR Center Grant [835737] FX The authors acknowledge support from the National Institutes of Health (T32 HL007936-12, RO1HL093282, and R21 EB016381) and the Environmental Protection Agency STAR Center Grant (#835737). The authors acknowledge Bernard Binder, PhD, Nicole Kleinstreuer, PhD, and Kevin Crofton, PhD for providing edits in the preparation of this manuscript. The authors also acknowledge the Waisman Center Cellular and Molecular Neuroscience Core and specifically Karla Knobel, PhD for supporting the laser scanning confocal microscopy experiments. NR 104 TC 0 Z9 0 U1 4 U2 20 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1742-7061 EI 1878-7568 J9 ACTA BIOMATER JI Acta Biomater. PD JUL 15 PY 2016 VL 39 BP 12 EP 24 DI 10.1016/j.actbio.2016.05.020 PG 13 WC Engineering, Biomedical; Materials Science, Biomaterials SC Engineering; Materials Science GA DQ1NA UT WOS:000378966500002 PM 27181878 ER PT J AU Cantwell, MG Katz, DR Sullivan, JC Borci, T Chen, RF AF Cantwell, Mark G. Katz, David R. Sullivan, Julia C. Borci, Todd Chen, Robert F. TI Caffeine in Boston Harbor past and present, assessing its utility as a tracer of wastewater contamination in an urban estuary SO MARINE POLLUTION BULLETIN LA English DT Article DE Wastewater; Caffeine; Tracer; Boston Harbor; CSOs ID ANTHROPOGENIC MARKER; SURFACE WATERS; SEWAGE; SEAWATER AB Sites throughout Boston Harbor were analyzed for caffeine to assess its utility as a tracer in identifying sources of sanitary wastewater. Caffeine ranged from 15 ng/L in the outer harbor to a high of 185 ng/L in the inner harbor. Inner harbor concentrations were a result of combined sewage overflow (CSO) events as well as illicit discharge of sanitary sewage into municipal storm drains. Comparing current results to data from 1998 to 1999 shows reductions in caffeine levels. Reductions are attributed to termination of effluent discharge to the harbor, declines in the number of CSOs and discharge volume along with efforts to eliminate illicit discharges. Spatial distributions of caffeine identified CSOs as major contemporary sources to the inner harbor. The findings further establish the utility of caffeine as a tracer for sanitary wastewater contamination in urban estuaries and demonstrate the efficacy of pollution reduction strategies undertaken in recent decades in Boston Harbor. Published by Elsevier Ltd. C1 [Cantwell, Mark G.; Katz, David R.] US EPA, Off Res & Dev, Narragansett, RI 02882 USA. [Sullivan, Julia C.] Oak Ridge Inst Sci & Educ, Narragansett, RI 02882 USA. [Borci, Todd] US EPA, Reg 1, Boston, MA 02109 USA. [Chen, Robert F.] Univ Massachusetts, Boston Sch Environm, Boston, MA 02125 USA. RP Cantwell, MG (reprint author), US EPA, Off Res & Dev, Narragansett, RI 02882 USA. EM cantwell.mark@epa.gov FU research Participation Program for the U.S. Environmental Protection Agency; Office of Research and Development; U.S. Environmental Protection Agency; Atlantic Ecology Division of the United States Environmental Protection Agency [ORD-014490]; Office Research and Development; National Health Environmental Effects Research Laboratory FX The authors thank Drs.Diane Nacci,Richard pruell and Mr.Steven Rego for their technical reviews. This research was supported in part by an apponintment to the research Participation Program for the U.S. Environmental Protection Agency, Office of Research and Development, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA.Although research described in this article has been wholly funded by the U.S. Environmental Protection Agency and has been technically reviewed at the Atlantic Ecology Division, it has not been subjected to Agency-level review. Therefore, it does not necessarily reflect the views of the Agency.This manuscript is contribution number ORD-014490 of the Atlantic Ecology Division of the United States Environmental Protection Agency, Office Research and Development, National Health Environmental Effects Research Laboratory. Mention of trade names does not constitute endorsement or recommendation for use. NR 13 TC 1 Z9 2 U1 9 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0025-326X EI 1879-3363 J9 MAR POLLUT BULL JI Mar. Pollut. Bull. PD JUL 15 PY 2016 VL 108 IS 1-2 BP 321 EP 324 DI 10.1016/j.marpolbul.2016.04.006 PG 4 WC Environmental Sciences; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DQ3LD UT WOS:000379102900049 PM 27085595 ER PT J AU Sahle-Demessie, E Han, C Zhao, A Hahn, B Grecsek, H AF Sahle-Demessie, E. Han, Changseok Zhao, Amy Hahn, Bill Grecsek, Heidi TI Interaction of engineered nanomaterials with hydrophobic organic pollutants SO NANOTECHNOLOGY LA English DT Article DE hydrophobic pollutants; ENM-pollutant adsorption; partitioning coefficient; TGA/GC/MS ID CARBON NANOTUBES; TITANIA NANOPARTICLES; ADSORPTION; AGGREGATION; ENVIRONMENT; EXTRACTION; DIFFERENCE; SEDIMENTS; ATRAZINE; ANATASE AB As nanomaterials become an increasing part of everyday consumer products, it is imperative to monitor their potential release during production, use and disposal, and to assess their impact on the health of humans and the ecosystem. This necessitates research to better understand how the properties of engineered nanomaterials (ENMs) lead to their accumulation and redistribution in the environment, and to assess whether they could become novel pollutants or if they can affect the mobility and bioavailability of other toxins. This study focuses on understanding the influence of nanostructured-TiO2 and the interaction of multi-walled carbon nanotubes with organic pollutants in water. We studied the adsorption and water phase dispersion of model pollutants with relatively small water solubility (i.e., two-and three-ring polyaromatic hydrocarbons and insecticides) with respect to ENMs. The sorption of pollutants was measured based on water phase analysis, and by separating suspended particles from the water phase and analyzing dried samples using integrated thermal-chromatographic-mass spectroscopic (TGA/GC/MS) techniques. Solid phase analysis using a combination of TGA/GC/MS is a novel technique that can provide real-time quantitative analysis and which helps to understand the interaction of hydrophobic organic pollutants and ENMs. The adsorption of these contaminants to nanomaterials increased the concentration of the contaminants in the aqueous phase as compared to the 'real' partitioning due to the octanol-water partitioning. The study showed that ENMs can significantly influence the adsorption and dispersion of hydrophobic/low water soluble contaminants. The type of ENM, the exposure to light, and the water pH have a significant influence on the partitioning of pollutants. C1 [Sahle-Demessie, E.; Han, Changseok; Zhao, Amy] US EPA, Off Res & Dev, Natl Risk Management Lab, Cincinnati, OH 45268 USA. [Hahn, Bill; Grecsek, Heidi] Perkin Elmer Inc, 710 Bridgeport Ave, Shelton, CT 06484 USA. RP Sahle-Demessie, E (reprint author), US EPA, Off Res & Dev, Natl Risk Management Lab, Cincinnati, OH 45268 USA. NR 41 TC 0 Z9 0 U1 20 U2 20 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 0957-4484 EI 1361-6528 J9 NANOTECHNOLOGY JI Nanotechnology PD JUL 15 PY 2016 VL 27 IS 28 AR 284003 DI 10.1088/0957-4484/27/28/284003 PG 10 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied SC Science & Technology - Other Topics; Materials Science; Physics GA DO0XU UT WOS:000377503100005 PM 27265536 ER PT J AU Starr, JM Li, WW Graham, SE Bradham, KD Stout, DM Williams, A Sylva, J AF Starr, James M. Li, Weiwei Graham, Stephen E. Bradham, Karen D. Stout, Daniel M., II Williams, Alan Sylva, Jason TI Using paired soil and house dust samples in an in vitro assay to assess the post ingestion bioaccessibility of sorbed fipronil SO JOURNAL OF HAZARDOUS MATERIALS LA English DT Article DE Fipronil; Bioaccessibility; Ingestion; Soils; House dusts ID DEGRADATION-PRODUCTS; PESTICIDES; FATE; METABOLITES; BEHAVIOR; SORPTION; INDOOR; FIELD; SIZE AB For children, ingestion of soils and house dusts can be an important exposure pathway for regulated organic compounds. Following ingestion, the extent to which compounds desorb and become bioaccessible is a critical determinant of systemic adsorption. We characterized the physicochemical properties of 37 soil and house dust pairs collected during a national survey of United States homes. For each sample, we measured the bioaccessibility of fipronil, a phenylpyrazole insecticide using an in vitro, three- compartment digestive system, then modeled the physicochemical predictors of fipronil bioaccessibility. The properties of the soils and dusts were not correlated and percent carbon was the only significant predictor of bioaccessibility for both soils (p < 0.001) and dusts (p < 0.001). The carbon content of the soils (3.1 +/- 2.4%) was lower than that of the dusts (18.6 +/- 6.9%) Due to the lower carbon content, soil sorbed fipronil was more bioaccessible than dust sorbed fipronil. However, the slope of the bioaccessibility carbon regression line was steeper for the soils than for the house dusts. This suggested that, for soils having carbon percentages greater than those in this study, fipronil bioaccessibility may be less than that of house dusts having equal carbon content. Published by Elsevier B.V. C1 [Starr, James M.; Bradham, Karen D.; Stout, Daniel M., II] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Li, Weiwei] US EPA, Oak Ridge Inst Sci & Educ, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Graham, Stephen E.] US EPA, Off Air Qual Planning & Stand, Res Triangle Pk, NC 27711 USA. [Williams, Alan; Sylva, Jason] US EPA, Natl Exposure Res Lab, Las Vegas, NV 89119 USA. RP Starr, JM (reprint author), US EPA, MD D205-05,109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM starr.james@epa.gov FU United States Environmental Protection Agency through its Office of Research and Development FX This work was supported by The United States Environmental Protection Agency through its Office of Research and Development who funded and managed the research described here. It has been subjected to Agency administrative review and approved for publication. This does not signify that the contents necessarily reflect the views and policies of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 32 TC 0 Z9 0 U1 4 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3894 EI 1873-3336 J9 J HAZARD MATER JI J. Hazard. Mater. PD JUL 15 PY 2016 VL 312 BP 141 EP 149 DI 10.1016/j.jhazmat.2016.03.053 PG 9 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DN1AU UT WOS:000376799000016 PM 27017400 ER PT J AU Hathaway, JM Winston, RJ Brown, RA Hunt, WF McCarthy, DT AF Hathaway, J. M. Winston, R. J. Brown, R. A. Hunt, W. F. McCarthy, D. T. TI Temperature dynamics of stormwater runoff in Australia and the USA SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE First flush; Temperature; Urban runoff; Thermal pollution ID TOTAL SUSPENDED-SOLIDS; URBAN HEAT-ISLAND; 1ST FLUSH; INDICATOR BACTERIA; POLLUTANT MASS; VARIABILITY; URBANIZATION; PERFORMANCE; CATCHMENT; SURFACES AB Thermal pollution of surface waters by urban stormwater runoff is an often overlooked by-product of urbanization. Elevated stream temperatures due to an influx of stormwater runoff can be detrimental to stream biota, in particular for cold water systems. However, few studies have examined temperature trends throughout storm events to determine how these thermal inputs are temporally distributed. In this study, six diverse catchments in two continents are evaluated for thermal dynamics. Summary statistics from the data showed larger catchments have lower maximum runoff temperatures, minimum runoff temperatures, and temperature variability. This reinforces the understanding that subsurface drainage infrastructure in urban catchments acts to moderate runoff temperatures. The catchments were also evaluated for the presence of a thermal first flush using two methodologies. Results showed the lack of a first flush under traditional assessment methodologies across all six catchments, supporting the results from a limited number of studies in literature. However, the time to peak temperature was not always coincident with the time to peak flow, highlighting the variability of thermal load over time. When a new first flush methodology was applied, significant differences in temperature were noted with increasing runoff depth for five of the six sites. This study is the first to identify a runoff temperature first flush, and highlights the need to carefully consider the appropriate methodology for such analyses. (C) 2016 Elsevier B.V. All rights reserved. C1 [Hathaway, J. M.] Univ Tennessee, Knoxville, TN USA. [Winston, R. J.; Hunt, W. F.] N Carolina State Univ, Raleigh, NC 27695 USA. [Brown, R. A.] US EPA, Edison, NJ USA. [McCarthy, D. T.] Monash Univ, Environm & Publ Hlth Microbiol Lab, Dept Civil Engn, Melbourne, Vic 3004, Australia. RP Hathaway, JM (reprint author), Univ Tennessee, Knoxville, TN USA. EM hathaway@utk.edu FU EPA [319]; North Carolina Department of Environment and Natural Resources; Melbourne Water through the Linkage Program [LP120100718] FX The authors appreciate the funding of the EPA 319 program for the data collected at both the Louisburg and Nashville sites, and recognize the North Carolina Department of Environment and Natural Resources for funding the data collection in Raleigh. We would like to acknowledge the contributions made by the Australian Research Council and Melbourne Water through the Linkage Program (LP120100718) for the collection and analysis of data for the Australian sites. NR 48 TC 0 Z9 0 U1 15 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUL 15 PY 2016 VL 559 BP 141 EP 150 DI 10.1016/j.scitotenv.2016.03.155 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK7WD UT WOS:000375136600016 PM 27058133 ER PT J AU Vandenberg, LN Agerstrand, M Beronius, A Beausoleil, C Bergman, A Bero, LA Bornehag, CG Boyer, CS Cooper, GS Cotgreave, I Gee, D Grandjean, P Guyton, KZ Hass, U Heindel, JJ Jobling, S Kidd, KA Kortenkamp, A Macleod, MR Martin, OV Norinder, U Scheringer, M Thayer, KA Toppari, J Whaley, P Woodruff, TJ Ruden, C AF Vandenberg, Laura N. Agerstrand, Marlene Beronius, Anna Beausoleil, Claire Bergman, Ake Bero, Lisa A. Bornehag, Carl-Gustaf Boyer, C. Scott Cooper, Glinda S. Cotgreave, Ian Gee, David Grandjean, Philippe Guyton, Kathryn Z. Hass, Ulla Heindel, Jerrold J. Jobling, Susan Kidd, Karen A. Kortenkamp, Andreas Macleod, Malcolm R. Martin, Olwenn V. Norinder, Ulf Scheringer, Martin Thayer, Kristina A. Toppari, Jorma Whaley, Paul Woodruff, Tracey J. Ruden, Christina TI A proposed framework for the systematic review and integrated assessment (SYRINA) of endocrine disrupting chemicals SO ENVIRONMENTAL HEALTH LA English DT Article DE Endocrine disrupting chemicals; Systematic review; Study evaluation; Strength of evidence; Weight of evidence; Adverse effect; Endocrine disrupting activity; Evidence integration; Epidemiology; In vivo ID EVIDENCE-BASED MEDICINE; ENVIRONMENTAL-HEALTH SCIENCE; RISK-ASSESSMENT; PUBLIC-HEALTH; FETAL-GROWTH; DEVELOPMENTAL ORIGINS; SCIENTIFIC STATEMENT; EVALUATION CRITERIA; DISEASE; GUIDE AB Background: The issue of endocrine disrupting chemicals (EDCs) is receiving wide attention from both the scientific and regulatory communities. Recent analyses of the EDC literature have been criticized for failing to use transparent and objective approaches to draw conclusions about the strength of evidence linking EDC exposures to adverse health or environmental outcomes. Systematic review methodologies are ideal for addressing this issue as they provide transparent and consistent approaches to study selection and evaluation. Objective methods are needed for integrating the multiple streams of evidence (epidemiology, wildlife, laboratory animal, in vitro, and in silico data) that are relevant in assessing EDCs. Methods: We have developed a framework for the systematic review and integrated assessment (SYRINA) of EDC studies. The framework was designed for use with the International Program on Chemical Safety (IPCS) and World Health Organization (WHO) definition of an EDC, which requires appraisal of evidence regarding 1) association between exposure and an adverse effect, 2) association between exposure and endocrine disrupting activity, and 3) a plausible link between the adverse effect and the endocrine disrupting activity. Results: Building from existing methodologies for evaluating and synthesizing evidence, the SYRINA framework includes seven steps: 1) Formulate the problem; 2) Develop the review protocol; 3) Identify relevant evidence; 4) Evaluate evidence from individual studies; 5) Summarize and evaluate each stream of evidence; 6) Integrate evidence across all streams; 7) Draw conclusions, make recommendations, and evaluate uncertainties. The proposed method is tailored to the IPCS/WHO definition of an EDC but offers flexibility for use in the context of other definitions of EDCs. Conclusions: When using the SYRINA framework, the overall objective is to provide the evidence base needed to support decision making, including any action to avoid/minimise potential adverse effects of exposures. This framework allows for the evaluation and synthesis of evidence from multiple evidence streams. Finally, a decision regarding regulatory action is not only dependent on the strength of evidence, but also the consequences of action/inaction, e.g. limited or weak evidence may be sufficient to justify action if consequences are serious or irreversible. C1 [Agerstrand, Marlene; Bergman, Ake; Ruden, Christina] Stockholm Univ, Dept Environm Sci & Analyt Chem, Stockholm, Sweden. [Vandenberg, Laura N.] Univ Massachusetts, Amherst Sch Publ Hlth & Hlth Sci, Dept Environm Hlth Sci, Amherst, MA USA. [Beronius, Anna] Karolinska Inst, Inst Environm Med, Stockholm, Sweden. [Beausoleil, Claire] ANSES French Agcy Food Environm & Occupat Hlth Sa, Maisons Alfort, France. [Bergman, Ake; Boyer, C. Scott; Norinder, Ulf] Swedish Toxicol Sci Res Ctr, Sodertalje, Sweden. [Bero, Lisa A.] Univ Sydney, Charles Perkins Ctr, Sydney, NSW, Australia. [Bornehag, Carl-Gustaf] Karlstad Univ, Dept Hlth Sci, Karlstad, Sweden. [Bornehag, Carl-Gustaf] Icahn Sch Med Mt Sinai, New York, NY USA. [Cooper, Glinda S.] US EPA, Washington, DC USA. [Cotgreave, Ian] Karolinska Inst, Swedish Toxicol Sci Res Ctr Swetox, Sodertalje, Sweden. [Gee, David; Jobling, Susan; Kortenkamp, Andreas; Martin, Olwenn V.] Brunel Univ London, Inst Environm Hlth & Societies, Uxbridge, Middx, England. [Grandjean, Philippe] Univ Southern Denmark, Dept Environm Med, Odense, Denmark. Int Agcy Res Canc, Lyon, France. Tech Univ Denmark, Natl Food Inst, Soborg, Denmark. [Heindel, Jerrold J.] Natl Inst Environm Hlth Sci, Div Extramural Res & Training, Res Triangle Pk, NC USA. [Kidd, Karen A.] Univ New Brunswick, Dept Biol, St John, NB, Canada. [Kidd, Karen A.] Univ New Brunswick, Canadian Rivers Inst, St John, NB, Canada. [Macleod, Malcolm R.] Univ Edinburgh, Ctr Clin Brain Sci, Edinburgh, Midlothian, Scotland. [Scheringer, Martin] ETH, Inst Chem & Bioengn, Zurich, Switzerland. [Thayer, Kristina A.] Natl Inst Environm Hlth Sci, Dept Hlth & Human Serv, Div Natl Toxicol Program, NIH, Res Triangle Pk, NC USA. [Toppari, Jorma] Univ Turku, Turku Univ Hosp, Turku, Finland. [Whaley, Paul] Univ Lancaster, Lancaster Environm Ctr, Lancaster, England. [Woodruff, Tracey J.] Univ Calif San Francisco, Sch Med, Program Reprod Hlth & Environm, Oakland, CA USA. RP Ruden, C (reprint author), Stockholm Univ, Dept Environm Sci & Analyt Chem, Stockholm, Sweden. EM christina.ruden@aces.su.se RI jobling, susan/N-9316-2016; OI Whaley, Paul/0000-0003-4021-0785; Macleod, Malcolm Robert/0000-0001-9187-9839; Grandjean, Philippe/0000-0003-4046-9658 FU Swedish Foundation for Strategic Environmental Research "Mistra"; National Institute of Environmental Health Sciences of the National Institutes of Health [K22ES025811]; Clarence Heller Foundation [A123547]; Passport Foundation; Forsythia Foundation; National Institute of Environmental Health Sciences [ES018135, ESO22841]; U.S. EPA STAR grants [RD83467801, RD83543301]; Academy of Finland; Sigrid Juselius; Danish EPA; Canada Research Chairs program [950-230607] FX The workshops that supported the writing of this manuscript were funded by the Swedish Foundation for Strategic Environmental Research "Mistra". LNV was funded by Award Number K22ES025811 from the National Institute of Environmental Health Sciences of the National Institutes of Health. TJW was funded by The Clarence Heller Foundation (A123547), the Passport Foundation, the Forsythia Foundation, the National Institute of Environmental Health Sciences (grants ES018135 and ESO22841), and U.S. EPA STAR grants (RD83467801 and RD83543301). JT was funded by the Academy of Finland and Sigrid Juselius. UH was funded by the Danish EPA. KAK was funded by the Canada Research Chairs program grant number 950-230607. NR 96 TC 1 Z9 1 U1 11 U2 26 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1476-069X J9 ENVIRON HEALTH-GLOB JI Environ. Health PD JUL 14 PY 2016 VL 15 AR 74 DI 10.1186/s12940-016-0156-6 PG 19 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DR6EG UT WOS:000379994300001 PM 27412149 ER PT J AU Dowling, AW Ruiz-Mercado, G Zavala, VM AF Dowling, Alexander W. Ruiz-Mercado, Gerardo Zavala, Victor M. TI A framework for multi-stakeholder decision-making and conflict resolution SO COMPUTERS & CHEMICAL ENGINEERING LA English DT Article DE Multi-stakeholder; Multiobjective; Optimization; Pareto optimality; Compromise; Conditional value-at-risk ID MULTIOBJECTIVE DYNAMIC OPTIMIZATION; NORMAL-BOUNDARY INTERSECTION; LIFE-CYCLE ASSESSMENT; SUSTAINABLE DESIGN; CHEMICAL-PROCESSES; PROGRAMMING APPROACH; COMPROMISE SOLUTION; GENETIC ALGORITHM; SUPPLY CHAINS; SYSTEMS AB We propose a decision-making framework to compute compromise solutions that balance conflicting priorities of multiple stakeholders on multiple objectives. In our setting, we shape the stakeholder dissatisfaction distribution by solving a conditional-value-at-risk (CVaR) minimization problem. The CVaR problem is parameterized by a probability level that shapes the tail of the dissatisfaction distribution. The proposed approach allows us to compute a family of compromise solutions and generalizes multi-stakeholder settings previously proposed in the literature that minimize average and worst-case dissatisfactions. We use the concept of the CVaR norm to give a geometric interpretation to this problem and use the properties of this norm to prove that the CVaR minimization problem yields Pareto optimal solutions for any choice of the probability level. We discuss a broad range of potential applications of the framework that involve complex decision-making processes. We demonstrate the developments using a biowaste facility location case study in which we seek to balance stakeholder priorities on transportation, safety, water quality, and capital costs. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Dowling, Alexander W.; Zavala, Victor M.] Univ Wisconsin, Dept Chem & Biol Engn, 1415 Engn Dr, Madison, WI 53706 USA. [Ruiz-Mercado, Gerardo] US EPA, Off Res & Dev, Natl Risk Management Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. RP Dowling, AW (reprint author), Univ Wisconsin, Dept Chem & Biol Engn, 1415 Engn Dr, Madison, WI 53706 USA. EM adowling2@wisc.edu FU U.S. Department of Energy Early Career program FX Victor M. Zavala acknowledges funding from the U.S. Department of Energy Early Career program and thanks Sanjay Mehrotra and Kibaek Kim for technical discussions that motivated this work. NR 74 TC 3 Z9 3 U1 8 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-1354 EI 1873-4375 J9 COMPUT CHEM ENG JI Comput. Chem. Eng. PD JUL 12 PY 2016 VL 90 BP 136 EP 150 DI 10.1016/j.compchemeng.2016.03.034 PG 15 WC Computer Science, Interdisciplinary Applications; Engineering, Chemical SC Computer Science; Engineering GA DM5QW UT WOS:000376406100010 ER PT J AU Goossens, ME Isa, F Brinkman, M Mak, D Reulen, R Wesselius, A Benhamou, S Bosetti, C Bueno-de-Mesquita, B Carta, A Allam, MF Golka, K Grant, EJ Jiang, XJ Johnson, KC Karagas, MR Kellen, E La Vecchia, C Lu, CM Marshall, J Moysich, K Pohlabeln, H Porru, S Steineck, G Stern, MC Tang, L Taylor, JA van den Brandt, P Villeneuve, PJ Wakai, K Weiderpass, E White, E Wolk, A Zhang, ZF Buntinx, F Zeegers, MP AF Goossens, Maria E. Isa, Fatima Brinkman, Maree Mak, David Reulen, Raoul Wesselius, Anke Benhamou, Simone Bosetti, Cristina Bueno-de-Mesquita, Bas Carta, Angela Allam, Md Farouk Golka, Klaus Grant, Eric J. Jiang, Xuejuan Johnson, Kenneth C. Karagas, Margaret R. Kellen, Eliane La Vecchia, Carlo Lu, Chih-Ming Marshall, James Moysich, Kirsten Pohlabeln, Hermann Porru, Stefano Steineck, Gunnar Stern, Marianne C. Tang, Li Taylor, Jack A. van den Brandt, Piet Villeneuve, Paul J. Wakai, Kenji Weiderpass, Elisabete White, Emily Wolk, Alicja Zhang, Zuo-Feng Buntinx, Frank Zeegers, Maurice P. TI International pooled study on diet and bladder cancer: the bladder cancer, epidemiology and nutritional determinants (BLEND) study: design and baseline characteristics SO ARCHIVES OF PUBLIC HEALTH LA English DT Article DE Bladder cancer; Diet; Risk; Pooled analysis ID RISK-FACTORS; FLUID INTAKE; CONSUMPTION; RATIONALE; SMOKING; COHORT; POLYMORPHISMS; VITAMINS; SYSTEM; FRUIT AB Background: In 2012, more than 400,000 urinary bladder cancer cases occurred worldwide, making it the 7th most common type of cancer. Although many previous studies focused on the relationship between diet and bladder cancer, the evidence related to specific food items or nutrients that could be involved in the development of bladder cancer remains inconclusive. Dietary components can either be, or be activated into, potential carcinogens through metabolism, or act to prevent carcinogen damage. Methods/design: The BLadder cancer, Epidemiology and Nutritional Determinants (BLEND) study was set up with the purpose of collecting individual patient data from observational studies on diet and bladder cancer. In total, data from 11,261 bladder cancer cases and 675,532 non-cases from 18 case-control and 6 cohort studies from all over the world were included with the aim to investigate the association between individual food items, nutrients and dietary patterns and risk of developing bladder cancer. Discussion: The substantial number of cases included in this study will enable us to provide evidence with large statistical power, for dietary recommendations on the prevention of bladder cancer. C1 [Goossens, Maria E.; Buntinx, Frank] Katholieke Univ Leuven, ACHG KU Leuven, Dept Gen Practice, Kapucijnenvoer 33,Blok J,Bus 7001, B-3000 Leuven, Belgium. [Isa, Fatima; Mak, David; Reulen, Raoul] Univ Birmingham, Dept Publ Hlth Epidemiol & Biostat, Birmingham, W Midlands, England. [Brinkman, Maree] Canc Council Victoria, Melbourne, Vic, Australia. [Wesselius, Anke; Zeegers, Maurice P.] Maastricht Univ, NUTRIM Sch Nutr & Translat Res Metab, Maastricht, Netherlands. [Benhamou, Simone] Fondat Jean Dausset CEPH, Variabilite Genet & Malad Humaines, INSERM U946, Paris, France. [Bosetti, Cristina] Ist Ric Farmacol Mario Negri, Lab Gen Epidemiol, Milan, Italy. [Bueno-de-Mesquita, Bas] Natl Inst Publ Hlth & Environm RIVM, DCD, Bilthoven, Netherlands. [Bueno-de-Mesquita, Bas] Univ Med Ctr, Gastroenterol & Hepatol, Utrecht, Netherlands. [Bueno-de-Mesquita, Bas] Univ London Imperial Coll Sci Technol & Med, Sch Publ Hlth, Epidemiol & Biostat, London, England. [Bueno-de-Mesquita, Bas] Univ Malaya, Social & Prevent Med, Fac Med, Kuala Lumpur, Malaysia. [Carta, Angela; Porru, Stefano] Univ Brescia, Sect Publ Hlth & Human Sci, Dept Med & Surg Specialties Radiol Sci & Publ Hlt, Brescia, Italy. [Allam, Md Farouk] Univ Cordoba, Dept Prevent Med & Publ Hlth, Fac Med, Cordoba, Spain. [Golka, Klaus] TU Dortmund, Leibniz Res Ctr Working Environm & Human Factors, Dortmund, Germany. [Grant, Eric J.] Dept Epidemiol Radiat Effects Res Fdn, Hiroshima, Japan. [Jiang, Xuejuan; Stern, Marianne C.] Univ So Calif, Dept Prevent Med, Los Angeles, CA 90089 USA. [Johnson, Kenneth C.] Univ Ottawa, Dept Epidemiol & Community Med, Ottawa, ON, Canada. [Karagas, Margaret R.] Geisel Sch Med Dartmouth, Dept Epidemiol, Hanover, NH USA. [Kellen, Eliane] Leuven Univ Ctr Canc Prevent LUCK, Leuven, Belgium. [La Vecchia, Carlo] Univ Milan, Dept Clin Med & Community Hlth, Milan, Italy. [Lu, Chih-Ming] Buddhist Dalin Tzu Chi Gen Hosp, Dept Urol, Dalin Township 62247, Chiayi County, Taiwan. [Marshall, James; Moysich, Kirsten; Tang, Li] Roswell Pk Canc Inst, Dept Canc Prevent & Control, Buffalo, NY 14263 USA. [Pohlabeln, Hermann] Leibniz Inst Prevent Res & Epidemiol BIPS, Bremen, Germany. [Steineck, Gunnar] Karolinska Hosp, Div Clin Canc Epidemiol, Dept Oncol & Pathol, Stockholm, Sweden. [Taylor, Jack A.] Natl Inst Environm Hlth Sci, Epidemiol Branch, NIH, Res Triangle Pk, NC USA. [Taylor, Jack A.] Natl Inst Environm Hlth Sci, Epigenet & Stem Cell Biol Lab, NIH, Res Triangle Pk, NC USA. [van den Brandt, Piet] Maastricht Univ, Dept Epidemiol, Sch Oncol & Dev Biol, Med Ctr, Maastricht, Netherlands. [van den Brandt, Piet] Maastricht Univ, Med Ctr, Sch Publ Hlth & Primary Care, Maastricht, Netherlands. [Villeneuve, Paul J.] Populat Studies Div Hlth Canada, Ottawa, ON, Canada. [Wakai, Kenji] Nagoya Univ, Dept Prevent Med, Grad Sch Med, Nagoya, Aichi, Japan. [Weiderpass, Elisabete] Karolinska Inst, Dept Med Epidemiol & Biostat, Med Epidemiol, Stockholm, Sweden. [Weiderpass, Elisabete] Canc Registry Norway, Inst Populat Based Canc Res, Res, Oslo, Norway. [Weiderpass, Elisabete] Folkhalsan Res Ctr, Genet Epidemiol Grp, Helsinki, Finland. [Weiderpass, Elisabete] Univ Tromso, Arctic Univ Norway, Dept Community Med, Tromso, Norway. [White, Emily] Fred Hutchinson Canc Res Ctr, 1124 Columbia St, Seattle, WA 98104 USA. [Wolk, Alicja] Karolinska Inst, Inst Environm Med, Div Nutr Epidemiol, Stockholm, Sweden. [Zhang, Zuo-Feng] Univ Calif Los Angeles, Fielding Sch Publ Hlth, UCLA Ctr Environm Genom, Dept Epidemiol, Los Angeles, CA USA. [Buntinx, Frank; Zeegers, Maurice P.] Maastricht Univ, CAPHRI Sch Publ Hlth & Primary Care, Maastricht, Netherlands. [Zeegers, Maurice P.] Univ Birmingham, Sch Canc Sci, Birmingham, W Midlands, England. RP Goossens, ME (reprint author), Katholieke Univ Leuven, ACHG KU Leuven, Dept Gen Practice, Kapucijnenvoer 33,Blok J,Bus 7001, B-3000 Leuven, Belgium. EM mieke.goossens@med.kuleuven.be RI Benhamou, Simone/K-6554-2015; Weiderpass, Elisabete/M-4029-2016; OI Weiderpass, Elisabete/0000-0003-2237-0128; La Vecchia, Carlo/0000-0003-1441-897X; taylor, jack/0000-0001-5303-6398 FU NCI NIH HHS [R01 CA057494, R01 CA074846, P30 CA016056, T32 CA009142, U01 CA096116]; NIEHS NIH HHS [P42 ES007373] NR 40 TC 0 Z9 0 U1 1 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0778-7367 EI 2049-3258 J9 ARCH PUBLIC HEALTH JI Arch. Public Health PD JUL 6 PY 2016 VL 74 AR 30 DI 10.1186/s13690-016-0140-1 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DS0ZI UT WOS:000380325100001 PM 27386115 ER PT J AU Daiber, EJ DeMarini, DM Ravuri, SA Liberatore, HK Cuthbertson, AA Thompson-Klemish, A Byer, JD Schmid, JE Afifi, MZ Blatchley, ER Richardson, SD AF Daiber, Eric J. DeMarini, David M. Ravuri, Sridevi A. Liberatore, Hannah K. Cuthbertson, Amy A. Thompson-Klemish, Alexis Byer, Jonathan D. Schmid, Judith E. Afifi, Mehrnaz Z. Blatchley, Ernest R., III Richardson, Susan D. TI Progressive Increase in Disinfection Byproducts and Mutagenicity from Source to Tap to Swimming Pool and Spa Water: Impact of Human Inputs SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID DRINKING-WATER; CHLORINATED POOL; OCCUPATIONAL-EXPOSURE; HALOACETIC ACIDS; BLADDER-CANCER; N-NITROSAMINES; INDOOR; TRICHLORAMINE; TRIHALOMETHANES; AIR AB Pools and spas are enjoyed throughout the world for exercise and relaxation. However, there are no previous studies on mutagenicity of disinfected spa (hot tub) waters or comprehensive identification of disinfection by-products (DBPs) formed in spas. Using 28 water samples from seven sites, we report the first integrated mutagenicity and comprehensive analytical chemistry of spas treated with chlorine, bromine, or ozone, along with pools treated with these same disinfectants. Gas chromatography (GC) with high resolution mass spectrometry, membrane-introduction mass spectrometry, and GC-electron capture detection were used to comprehensively identify and quantify DBPs and other contaminants. Mutagenicity was assessed by the Salmonella mutagenicity assay. More than 100 DBPs were identified, including a new class of DBPs, bromoimidazoles. Organic extracts of brominated pool/spa waters were 1.8X than chlorinated ones; spa waters were 1.7X more mutagenic than pools. Pool and spa samples were 2.4 and 4.1X more mutagenic, respectively, than corresponding tap waters. The concentration of the sum of 21 DBPs measured quantitatively increased from finished to tap to pool to spa; and mutagenic potency increased from finished/tap to pools to spas. Mutagenic potencies of samples from a chlorinated site correlated best with brominated haloacetic acid concentrations (Br-HAAs) (r = 0.98) and nitrogen-containing DBPs (N-DBPs) (r = 0.97) and the least with Br-trihalomethanes (r = 0.29) and Br N-DBPs (r = 0.04). The mutagenic potencies of samples from a brominated site correlated best (r = 0.82) with the concentrations of the nine HAAs, Br-HAAs, and Br-DBPs. Human use increased significantly the DBP concentrations and mutagenic potencies for most pools and spas. These data provide evidence that human precursors can increase mutagenic potencies of pools and spas and that this increase is associated with increased DBP concentrations. C1 [Daiber, Eric J.; Ravuri, Sridevi A.] US EPA, Student Serv Author, Natl Exposure Res Lab, Athens, GA 30605 USA. [DeMarini, David M.; Schmid, Judith E.] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Liberatore, Hannah K.; Cuthbertson, Amy A.; Thompson-Klemish, Alexis; Richardson, Susan D.] Univ South Carolina, Dept Chem & Biochem, 631 Sumter St, Columbia, SC 29208 USA. [Byer, Jonathan D.] LECO Corp, 3000 Lakeview Ave, St Joseph, MI 49085 USA. [Afifi, Mehrnaz Z.; Blatchley, Ernest R., III] Purdue Univ, Lyles Sch Civil Engn, 550 Stadium Mall Dr, W Lafayette, IN 47907 USA. [Blatchley, Ernest R., III] Purdue Univ, Div Environm & Ecol Engn, 500 Cent Dr, W Lafayette, IN 47907 USA. [Daiber, Eric J.] US EPA, Natl Risk Management Res Lab, Oak Ridge Associated Univ, Ada, OK 74820 USA. [Ravuri, Sridevi A.] PG Assist Serv Ltd, Durham S47 0PS, England. RP Richardson, SD (reprint author), Univ South Carolina, Dept Chem & Biochem, 631 Sumter St, Columbia, SC 29208 USA. EM richardson.susan@sc.edu RI Liberatore, Hannah/R-1317-2016 OI Liberatore, Hannah/0000-0001-7423-3251 NR 73 TC 1 Z9 1 U1 24 U2 45 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 5 PY 2016 VL 50 IS 13 SI SI BP 6652 EP 6662 DI 10.1021/acs.est.6b00808 PG 11 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DQ7DE UT WOS:000379366300010 PM 27124361 ER PT J AU Melvin, AM Sarofim, MC Crimmins, AR AF Melvin, April M. Sarofim, Marcus C. Crimmins, Allison R. TI Climate Benefits of US EPA Programs and Policies That Reduced Methane Emissions 1993-2013 SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID NATURAL-GAS SYSTEMS; UNITED-STATES; ATMOSPHERIC METHANE; HEALTH; OZONE; POLLUTION; IMPACTS AB The United States (U.S.) Environmental Protection Agency (EPA) has established voluntary programs to reduce methane (CH4) emissions, and regulations that either directly reduce CH4 or provide co-benefits of reducing CH, emissions while controlling for other air pollutants. These programs and regulations address four sectors that are among the largest domestic CH4 emissions sources: municipal solid waste landfills, oil and natural gas, coal mining, and agricultural manure management. Over the 1993-2013 time period, 127.9 Tg of CH4 emissions reductions were attributed to these programs, equal to about 18% of the counterfactual (or potential) domestic emissions over that time, with almost 70% of the abatement due to landfill sector regulations. Reductions attributed to the voluntary programs increased nearly continuously during the study period. We quantified how these reductions influenced atmospheric CH4 concentration and global temperature, finding a decrease in concentration of 28 ppb and an avoided temperature rise of 0.006 degrees C by 2013. Further, we monetized the climate and ozone-health impacts of the CH4 reductions, yielding an estimated benefit of $255 billion. These results indicate that EPA programs and policies have made a strong contribution to CH4 abatement, with climate and air quality benefits. C1 [Melvin, April M.; Sarofim, Marcus C.; Crimmins, Allison R.] US EPA, Climate Change Div, 1200 Penn Ave NW, Washington, DC 20460 USA. RP Sarofim, MC (reprint author), US EPA, Climate Change Div, 1200 Penn Ave NW, Washington, DC 20460 USA. EM sarofim.marcus@epa.gov NR 52 TC 0 Z9 0 U1 9 U2 22 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 5 PY 2016 VL 50 IS 13 SI SI BP 6873 EP 6881 DI 10.1021/acs.est.6b00367 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DQ7DE UT WOS:000379366300035 PM 27227378 ER PT J AU Briggs, MA Buckley, SF Bagtzoglou, AC Werkema, DD Lane, JW AF Briggs, Martin A. Buckley, Sean F. Bagtzoglou, Amvrossios C. Werkema, Dale D. Lane, John W., Jr. TI Actively heated high-resolution fiber-optic-distributed temperature sensing to quantify streambed flow dynamics in zones of strong groundwater upwelling SO WATER RESOURCES RESEARCH LA English DT Article ID SURFACE-WATER; TIME-SERIES; HYPORHEIC ZONES; THERMAL REFUGIA; FLUID-FLOW; DISCHARGE; EXCHANGE; RIVER; FIELD; DENITRIFICATION AB Zones of strong groundwater upwelling to streams enhance thermal stability and moderate thermal extremes, which is particularly important to aquatic ecosystems in a warming climate. Passive thermal tracer methods used to quantify vertical upwelling rates rely on downward conduction of surface temperature signals. However, moderate to high groundwater flux rates (>-1.5 m d(-1)) restrict downward propagation of diurnal temperature signals, and therefore the applicability of several passive thermal methods. Active streambed heating from within high-resolution fiber-optic temperature sensors (A-HRTS) has the potential to define multidimensional fluid-flux patterns below the extinction depth of surface thermal signals, allowing better quantification and separation of local and regional groundwater discharge. To demonstrate this concept, nine A-HRTS were emplaced vertically into the streambed in a grid with similar to 0.40 m lateral spacing at a stream with strong upward vertical flux in Mashpee, Massachusetts, USA. Long-term (8-9 h) heating events were performed to confirm the dominance of vertical flow to the 0.6 m depth, well below the extinction of ambient diurnal signals. To quantify vertical flux, short-term heating events (28 min) were performed at each A-HRTS, and heat-pulse decay over vertical profiles was numerically modeled in radial two dimension (2-D) using SUTRA. Modeled flux values are similar to those obtained with seepage meters, Darcy methods, and analytical modeling of shallow diurnal signals. We also observed repeatable differential heating patterns along the length of vertically oriented sensors that may indicate sediment layering and hyporheic exchange superimposed on regional groundwater discharge. C1 [Briggs, Martin A.; Buckley, Sean F.; Lane, John W., Jr.] US Geol Survey, Branch Geophys, Off Groundwater, Unit 5015, Storrs, CT 06269 USA. [Bagtzoglou, Amvrossios C.] Univ Connecticut, Dept Civil & Environm Engn, Unit 3037, Storrs, CT USA. [Werkema, Dale D.] US EPA, Off Res & Dev, Environm Measurement & Monitoring Div, Environm Chem Branch, Las Vegas, NV 89193 USA. RP Briggs, MA (reprint author), US Geol Survey, Branch Geophys, Off Groundwater, Unit 5015, Storrs, CT 06269 USA. EM mbriggs@usgs.gov FU U.S. Environmental Protection Agency; U.S. Geological Survey Groundwater Resources and Toxic Substance Hydrology Programs; U.S. Environmental Protection Agency through its Office of Research and Development [DW-14-92381701] FX The authors thank Yuri Rupert and Sarah Morton for assistance with the field experiments, the town of Mashpee, Massachusetts, for access to the field site, and Steve Hurley of the Massachusetts Fish and Wildlife Service for data and discussions related to brook trout habitat. We also thank the Editors at WRR, Lanbo Liu for input on an early version of this manuscript, and USGS reviewer Donald Rosenberry. Funding for these studies was provided by the U.S. Environmental Protection Agency and the U.S. Geological Survey Groundwater Resources and Toxic Substance Hydrology Programs. The U.S. Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here under assistance agreement number DW-14-92381701 to the USGS. The manuscript has been subjected to Agency review and approved for publication. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Data presented in this manuscript are available on the U.S. Geological Survey, Branch of Geophysics website (http://water.usgs.gov/ogw/bgas/). Quashnet River streamflow records can be found at: http://waterdata.usgs.gov/nwis. NR 67 TC 1 Z9 1 U1 0 U2 0 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD JUL PY 2016 VL 52 IS 7 BP 5179 EP 5194 DI 10.1002/2015WR018219 PG 16 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DW5KO UT WOS:000383683800013 ER PT J AU Thai, SF Wallace, KA Jones, CP Ren, HZ Grulke, E Castellon, BT Crooks, J Kitchin, KT AF Thai, Sheau-Fung Wallace, Kathleen A. Jones, Carlton P. Ren, Hongzu Grulke, Eric Castellon, Benjamin T. Crooks, James Kitchin, Kirk T. TI Differential Genomic Effects of Six Different TiO2 Nanomaterials on Human Liver HepG2 Cells SO JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY LA English DT Article DE Nanoparticles; Genomic effects; mRNA expression profiling; Signaling pathways; Nano-TiO2 ID TITANIUM-DIOXIDE NANOPARTICLES; IN-VITRO; SIGNALING PATHWAYS; ACUTE TOXICITY; CYTOTOXICITY; PARTICLES; ASSAY; MICE; NANO-TIO2; APOPTOSIS AB Human HepG2 cells were exposed to six TiO2 nanomaterials (with dry primary particle sizes ranging from 22 to 214 nm, either 0.3, 3, or 30 mu g/mL) for 3 days. Some of these canonical pathways changed by nano-TiO2 in vitro treatments have been already reported in the literature, such as NRF2-mediated stress response, fatty acid metabolism, cell cycle and apoptosis, immune response, cholesterol biosynthesis, and glycolysis. But this genomic study also revealed some novel effects such as protein synthesis, protein ubiquitination, hepatic fibrosis, and cancer-related signaling pathways. More importantly, this genomic analysis of nano-TiO2 treated HepG2 cells linked some of the in vitro canonical pathways to in vivo adverse outcomes: NRF2-mediated response pathways to oxidative stress, acute phase response to inflammation, cholesterol biosynthesis to steroid hormones alteration, fatty acid metabolism changes to lipid homeostasis alteration, G2/M cell checkpoint regulation to apoptosis, and hepatic fibrosis/stellate cell activation to liver fibrosis. (C) 2016 Wiley Periodicals, Inc. C1 [Thai, Sheau-Fung; Wallace, Kathleen A.; Jones, Carlton P.; Ren, Hongzu; Castellon, Benjamin T.; Crooks, James; Kitchin, Kirk T.] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Grulke, Eric] Univ Kentucky, Dept Chem & Mat Engn, Lexington, KY 40506 USA. RP Thai, SF (reprint author), US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. EM thai.sheau-fung@epa.gov RI Castellon, Benjamin/I-4117-2014 OI Castellon, Benjamin/0000-0001-8883-076X FU U. S. EPA FX The information in this document has been funded wholly by the U. S. EPA. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents necessarily reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 49 TC 1 Z9 1 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1095-6670 EI 1099-0461 J9 J BIOCHEM MOL TOXIC JI J. Biochem. Mol. Toxicol. PD JUL PY 2016 VL 30 IS 7 BP 331 EP 341 DI 10.1002/jbt.21798 PG 11 WC Biochemistry & Molecular Biology; Toxicology SC Biochemistry & Molecular Biology; Toxicology GA DU9YW UT WOS:000382575100002 PM 26918567 ER PT J AU Sparks, AM Kolden, CA Talhelm, AF Smith, AMS Apostol, KG Johnson, DM Boschetti, L AF Sparks, Aaron M. Kolden, Crystal A. Talhelm, Alan F. Smith, Alistair M. S. Apostol, Kent G. Johnson, Daniel M. Boschetti, Luigi TI Spectral Indices Accurately Quantify Changes in Seedling Physiology Following Fire: Towards Mechanistic Assessments of Post-Fire Carbon Cycling SO REMOTE SENSING LA English DT Article DE fire; remote sensing; severity; carbon; recovery; mortality ID NORMALIZED BURN RATIO; UNITED-STATES; WILDFIRE SEVERITY; REFLECTANCE; PHOTOSYNTHESIS; ECOSYSTEMS; MORTALITY; BIOMASS; NDVI; HYPOTHESIS AB Fire activity, in terms of intensity, frequency, and total area burned, is expected to increase with a changing climate. A challenge for landscape-level assessment of fire effects, often termed burn severity, is that current remote sensing assessments provide very little information regarding tree/vegetation physiological performance and recovery, limiting our understanding of fire effects on ecosystem services such as carbon storage/cycling. In this paper, we evaluated whether spectral indices common in vegetation stress and burn severity assessments could accurately quantify post-fire physiological performance (indicated by net photosynthesis and crown scorch) of two seedling species, Larix occidentalis and Pinus contorta. Seedlings were subjected to increasing fire radiative energy density (FRED) doses through a series of controlled laboratory surface fires. Mortality, physiology, and spectral reflectance were assessed for a month following the fires, and then again at one year post-fire. The differenced Normalized Difference Vegetation Index (dNDVI) spectral index outperformed other spectral indices used for vegetation stress and burn severity characterization in regard to leaf net photosynthesis quantification, indicating that landscape-level quantification of tree physiology may be possible. Additionally, the survival of the majority of seedlings in the low and moderate FRED doses indicates that fire-induced mortality is more complex than the currently accepted binary scenario, where trees survive with no impacts below a certain temperature and duration threshold, and mortality occurs above the threshold. C1 [Sparks, Aaron M.; Kolden, Crystal A.; Smith, Alistair M. S.; Johnson, Daniel M.; Boschetti, Luigi] Univ Idaho, Coll Nat Resources, Moscow, ID 83844 USA. [Sparks, Aaron M.; Kolden, Crystal A.; Smith, Alistair M. S.] Univ Idaho, IFIRE, Moscow, ID 83844 USA. [Talhelm, Alan F.] US EPA, Oak Ridge Inst Sci Educ, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. [Apostol, Kent G.] Univ Arizona, Coll Agr & Life Sci, Payson, AZ 85541 USA. RP Sparks, AM (reprint author), Univ Idaho, Coll Nat Resources, Moscow, ID 83844 USA.; Sparks, AM (reprint author), Univ Idaho, IFIRE, Moscow, ID 83844 USA. EM spar5010@vandals.uidaho.edu; ckolden@uidaho.edu; atalhelm@uidaho.edu; alistair@uidaho.edu; kapostol@gmail.com; danjohnson@uidaho.edu; luigi@uidaho.edu RI Boschetti, Luigi/C-6198-2008; Johnson, Daniel/E-6789-2011; OI Boschetti, Luigi/0000-0001-6525-4413; Johnson, Daniel/0000-0001-5890-3147; Smith, Alistair/0000-0003-0071-9958; Kolden, Crystal/0000-0001-7093-4552 FU National Aeronautics and Space Administration (NASA) [NNX11AO24G]; National Science Foundation [IOS-1146751, DEB-1251441, IIA-1301792]; National Science Foundation under Hazards SEES award [DMS-1520873]; Idaho Space Grant Consortium FX Seedlings were grown at the University of Idaho's Center for Forest Nursery and Seedling Research and combustion experiments were conducted within the Idaho Fire Initiative for Research and Education (IFIRE) combustion laboratory. This work was partially funded by the National Aeronautics and Space Administration (NASA) under award NNX11AO24G and the National Science Foundation under award IOS-1146751 to Dan Johnson. Partial support for Alan Talhelm was provided by the National Science Foundation under award DEB-1251441. Partial funding for this research for Aaron Sparks, Alistair Smith, and Crystal Kolden was provided by the National Science Foundation under Hazards SEES award DMS-1520873. Alistair Smith received partial support from the National Science Foundation award IIA-1301792. Aaron Sparks was additionally funded through the Idaho Space Grant Consortium. The views expressed in this paper are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. NR 62 TC 4 Z9 4 U1 7 U2 7 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD JUL PY 2016 VL 8 IS 7 AR UNSP 572 DI 10.3390/rs8070572 PG 13 WC Remote Sensing SC Remote Sensing GA DU5AR UT WOS:000382224800042 ER PT J AU Stephens, ML Betts, K Beck, NB Cogliano, V Dickersin, K Fitzpatrick, S Freeman, J Gray, G Hartung, T McPartland, J Rooney, AA Scherer, RW Verloo, D Hoffmann, S AF Stephens, Martin L. Betts, Kellyn Beck, Nancy B. Cogliano, Vincent Dickersin, Kay Fitzpatrick, Suzanne Freeman, James Gray, George Hartung, Thomas McPartland, Jennifer Rooney, Andrew A. Scherer, Roberta W. Verloo, Didier Hoffmann, Sebastian TI The Emergence of Systematic Review in Toxicology SO TOXICOLOGICAL SCIENCES LA English DT Review DE systematic review; risk of bias; data integration ID EVIDENCE-BASED MEDICINE; ENVIRONMENTAL-HEALTH SCIENCE; FETAL-GROWTH; EPIDEMIOLOGIC EVIDENCE; ARSENIC EXPOSURE; QUALITY; INTEGRATION; VALIDATION AB The Evidence-based Toxicology Collaboration hosted a workshop on "The Emergence of Systematic Review and Related Evidence-based Approaches in Toxicology," on November 21, 2014 in Baltimore, Maryland. The workshop featured speakers from agencies and organizations applying systematic review approaches to questions in toxicology, speakers with experience in conducting systematic reviews in medicine and healthcare, and stakeholders in industry, government, academia, and non-governmental organizations. Based on the workshop presentations and discussion, here we address the state of systematic review methods in toxicology, historical antecedents in both medicine and toxicology, challenges to the translation of systematic review from medicine to toxicology, and thoughts on the way forward. We conclude with a recommendation that as various agencies and organizations adapt systematic review methods, they continue to work together to ensure that there is a harmonized process for how the basic elements of systematic review methods are applied in toxicology. C1 [Stephens, Martin L.; Hartung, Thomas] Johns Hopkins Ctr Alternat Anim Testing, Baltimore, MD USA. [Beck, Nancy B.] Amer Chem Council, Washington, DC USA. [Cogliano, Vincent] US EPA, Arlington, VA USA. [Dickersin, Kay; Scherer, Roberta W.] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Baltimore, MD USA. [Fitzpatrick, Suzanne] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD USA. [Freeman, James] ExxonMobil Biomed Sci, Annandale, NJ USA. [Gray, George] George Washington Univ, Milken Inst Sch Publ Hlth, Washington, DC USA. [Hartung, Thomas] Univ Konstanz, CAAT Europe, Constance, Germany. [McPartland, Jennifer] Environm Def Fund, Washington, DC USA. [Rooney, Andrew A.] NIEHS, Off Hlth Assessment & Translat, Div Natl Toxicol Program, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Verloo, Didier] European Food Safety Author, I-43126 Parma, Italy. [Hoffmann, Sebastian] Seh Consulting Serv, D-33098 Paderborn, Germany. RP Stephens, ML (reprint author), Johns Hopkins Ctr Alternat Anim Testing, Baltimore, MD USA. EM msteph14@jhu.edu FU ExxonMobil Foundation FX This work was supported by funding from an anonymous private charitable foundation and the ExxonMobil Foundation to the Johns Hopkins Center for Alternatives to Animal Testing (CAAT). CAAT serves as the secretariat for the Evidence-based Toxicology Collaboration, which hosted the workshop on which this article is based. Only those authors working at or for CAAT (K.B., T.H., S.H., and M.L.S.) received compensation from these sources via CAAT for their participation in the workshop or their preparation of this article. NR 44 TC 4 Z9 4 U1 5 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD JUL PY 2016 VL 152 IS 1 BP 10 EP 16 DI 10.1093/toxsci/kfw059 PG 7 WC Toxicology SC Toxicology GA DT2AA UT WOS:000381282000002 PM 27208075 ER PT J AU El-Masri, H Kleinstreuer, N Hines, RN Adams, L Tal, T Isaacs, K Wetmore, BA Tan, YM AF El-Masri, Hisham Kleinstreuer, Nicole Hines, Ronald N. Adams, Linda Tal, Tamara Isaacs, Kristin Wetmore, Barbara A. Tan, Yu-Mei TI Integration of Life-Stage Physiologically Based Pharmacokinetic Models with Adverse Outcome Pathways and Environmental Exposure Models to Screen for Environmental Hazards SO TOXICOLOGICAL SCIENCES LA English DT Article DE PBPK; AOPs; life-stage; developmental toxicology; environmental toxicology ID BLOOD-FLOW; IN-VITRO; PBPK MODELS; HUMAN FETUS; DEVELOPMENTAL EXPRESSION; TOXCAST PROGRAM; CARDIAC-OUTPUT; DUCTUS VENOSUS; TOXICITY; DOSIMETRY AB A computational framework was developed to assist in screening and prioritizing chemicals based on their dosimetry, toxicity, and potential exposures. The overall strategy started with contextualizing chemical activity observed in high-throughput toxicity screening (HTS) by mapping these assays to biological events described in Adverse Outcome Pathways (AOPs). Next, in vitro to in vivo (IVIVE) extrapolation was used to convert an in vitro dose to an external exposure level, which was compared with potential exposure levels to derive an AOP-based margins of exposure (MOE). In this study, the framework was applied to estimate MOEs for chemicals that can potentially cause developmental toxicity following a putative AOP for fetal vasculogenesis/angiogenesis. A physiologically based pharmacokinetic (PBPK) model was developed to describe chemical disposition during pregnancy, fetal, neonatal, and infant to adulthood stages. Using this life-stage PBPK model, maternal exposures were estimated that would yield fetal blood levels equivalent to the chemical concentration that altered in vitro activity of selected HTS assays related to the most sensitive vasculogenesis/angiogenesis putative AOP. The resulting maternal exposure estimates were then compared with potential exposure levels using literature data or exposure models to derive AOP-based MOEs. C1 [El-Masri, Hisham; Hines, Ronald N.; Adams, Linda; Tal, Tamara] US EPA, Natl Human & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC USA. [Kleinstreuer, Nicole] Natl Inst Environm Hlth Sci, Natl Toxicol Program, Interagency Ctr Evaluat Alternat Toxicol Methods, Res Triangle Pk, NC USA. [Isaacs, Kristin; Tan, Yu-Mei] US EPA, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC USA. [Wetmore, Barbara A.] ScitoVation, Res Triangle Pk, NC USA. RP El-Masri, H (reprint author), US EPA, Natl Human & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC USA. EM el-masri.hisham@epa.gov OI Kleinstreuer, Nicole/0000-0002-7914-3682 NR 46 TC 0 Z9 0 U1 7 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD JUL PY 2016 VL 152 IS 1 BP 230 EP 243 DI 10.1093/toxsci/kfw082 PG 14 WC Toxicology SC Toxicology GA DT2AA UT WOS:000381282000020 PM 27208077 ER PT J AU Carlin, DJ Naujokas, MF Bradham, KD Cowden, J Heacock, M Henry, HF Lee, JS Thomas, DJ Thompson, C Tokar, EJ Waalkes, MP Birnbaum, LS Suk, WA AF Carlin, Danielle J. Naujokas, Marisa F. Bradham, Karen D. Cowden, John Heacock, Michelle Henry, Heather F. Lee, Janice S. Thomas, David J. Thompson, Claudia Tokar, Erik J. Waalkes, Michael P. Birnbaum, Linda S. Suk, William A. TI Arsenic and Environmental Health: State of the Science and Future Research Opportunities SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Review ID DIFFERENTIAL DNA METHYLATION; UMBILICAL-CORD BLOOD; FATTY LIVER-DISEASE; DRINKING-WATER; RISK-ASSESSMENT; IN-UTERO; GUT MICROBIOME; RELATIVE BIOAVAILABILITY; REMEDIATION TECHNIQUES; 21ST-CENTURY ROADMAP AB BACKGROUND: Exposure to inorganic and organic arsenic compounds is a major public health problem that affects hundreds of millions of people worldwide. Exposure to arsenic is associated with cancer and noncancer effects in nearly every organ in the body, and evidence is mounting for health effects at lower levels of arsenic exposure than previously thought. Building from a tremendous knowledge base with > 1,000 scientific papers published annually with "arsenic" in the title, the question becomes, what questions would best drive future research directions? OBJECTIVES: The objective is to discuss emerging issues in arsenic research and identify data gaps across disciplines. METHODS: The National Institutes of Health's National Institute of Environmental Health Sciences Superfund Research Program convened a workshop to identify emerging issues and research needs to address the multi-faceted challenges related to arsenic and environmental health. This review summarizes information captured during the workshop. DISCUSSION: More information about aggregate exposure to arsenic is needed, including the amount and forms of arsenic found in foods. New strategies for mitigating arsenic exposures and related health effects range from engineered filtering systems to phytogenetics and nutritional interventions. Furthermore, integration of omics data with mechanistic and epidemiological data is a key step toward the goal of linking biomarkers of exposure and susceptibility to disease mechanisms and outcomes. Conclusions: Promising research strategies and technologies for arsenic exposure and adverse health effect mitigation are being pursued, and future research is moving toward deeper collaborations and integration of information across disciplines to address data gaps. C1 [Carlin, Danielle J.; Heacock, Michelle; Henry, Heather F.; Suk, William A.] NIEHS, Superfund Res Program, NIH, US Dept HHS, Res Triangle Pk, NC 27560 USA. [Naujokas, Marisa F.] MDB Inc, Durham, NC USA. [Bradham, Karen D.] US EPA, Human Exposure & Atmospher Sci Div, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Cowden, John] US EPA, Natl Ctr Computat Toxicol, ORD, Res Triangle Pk, NC 27711 USA. [Lee, Janice S.] US EPA, Natl Ctr Environm Assessment, ORD, Res Triangle Pk, NC 27711 USA. [Thomas, David J.] US EPA, Integrated Syst Toxicol Div, Natl Human & Environm Hlth Effects Res Lab, ORD, Res Triangle Pk, NC 27711 USA. [Thompson, Claudia] NIEHS, Populat Hlth Branch, NIH, US Dept HHS, Res Triangle Pk, NC 27560 USA. [Tokar, Erik J.; Waalkes, Michael P.; Birnbaum, Linda S.] NIEHS, Natl Toxicol Program, NIH, US Dept HHS, Res Triangle Pk, NC 27560 USA. [Birnbaum, Linda S.] NIEHS, NIH, US Dept HHS, Res Triangle Pk, NC 27560 USA. RP Carlin, DJ (reprint author), NIEHS, Div Extramural Res & Training, Hazardous Subst Res Branch, Superfund Res Program, Keystone Bldg,530 Davis Dr,POB 12233, Res Triangle Pk, NC 27560 USA. EM danielle.carlin@nih.gov FU NIH, NIEHS [ES-102925] FX This work was supported in part by the NIH, NIEHS, including grant ES-102925. NR 130 TC 6 Z9 6 U1 33 U2 40 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD JUL PY 2016 VL 124 IS 7 BP 890 EP 899 DI 10.1289/ehp.1510209 PG 10 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DS4KF UT WOS:000380749300009 PM 26587579 ER PT J AU Shah, I Setzer, RW Jack, J Houck, KA Judson, RS Knudsen, TB Liu, J Martin, MT Reif, DM Richard, AM Thomas, RS Crofton, KM Dix, DJ Kavlock, RJ AF Shah, Imran Setzer, R. Woodrow Jack, John Houck, Keith A. Judson, Richard S. Knudsen, Thomas B. Liu, Jie Martin, Matthew T. Reif, David M. Richard, Ann M. Thomas, Russell S. Crofton, Kevin M. Dix, David J. Kavlock, Robert J. TI Using ToxCast (TM) Data to Reconstruct Dynamic Cell State Trajectories and Estimate Toxicological Points of Departure SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID TOXICITY; 21ST-CENTURY; ADVERSE; SYSTEMS; VISION AB BACKGROUND: High-content imaging (HCI) allows simultaneous measurement of multiple cellular phenotypic changes and is an important tool for evaluating the biological activity of chemicals. OBJECTIVES: Our goal was to analyze dynamic cellular changes using HCI to identify the "tipping point" at which the cells did not show recovery towards a normal phenotypic state. METHODS: HCI was used to evaluate the effects of 967 chemicals (in concentrations ranging from 0.4 to 200 mu M) on HepG2 cells over a 72-hr exposure period. The HCI end points included p53, c-Jun, histone H2A. x, alpha-tubulin, histone H3, alpha tubulin, mitochondrial membrane potential, mitochondrial mass, cell cycle arrest, nuclear size, and cell number. A computational model was developed to interpret HCI responses as cell-state trajectories. RESULTS: Analysis of cell-state trajectories showed that 336 chemicals produced tipping points and that HepG2 cells were resilient to the effects of 334 chemicals up to the highest concentration (200 mu M) and duration (72 hr) tested. Tipping points were identified as concentration-dependent transitions in system recovery, and the corresponding critical concentrations were generally between 5 and 15 times (25th and 75th percentiles, respectively) lower than the concentration that produced any significant effect on HepG2 cells. The remaining 297 chemicals require more data before they can be placed in either of these categories. CONCLUSIONS: These findings show the utility of HCI data for reconstructing cell state trajectories and provide insight into the adaptation and resilience of in vitro cellular systems based on tipping points. Cellular tipping points could be used to define a point of departure for risk-based prioritization of environmental chemicals. C1 [Shah, Imran; Setzer, R. Woodrow; Houck, Keith A.; Judson, Richard S.; Knudsen, Thomas B.; Martin, Matthew T.; Richard, Ann M.; Thomas, Russell S.; Crofton, Kevin M.; Dix, David J.; Kavlock, Robert J.] US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Jack, John] North Carolina State Univ, Dept Stat, Raleigh, NC USA. [Liu, Jie] US DOE, ORISE, Oak Ridge, TN USA. [Reif, David M.] North Carolina State Univ, Dept Biol Sci, Raleigh, NC USA. [Dix, David J.] US EPA, Off Sci Coordinat & Policy, Washington, DC 20460 USA. [Kavlock, Robert J.] US EPA, Off Res & Dev, Washington, DC 20460 USA. [Liu, Jie] US FDA, Ctr Food Safety & Appl Nutr, Off Food Addit Safety, College Pk, MD USA. RP Shah, I (reprint author), US EPA, 109 TW Alexander Dr B205-01, Res Triangle Pk, NC 27711 USA. EM Shah.Imran@epa.gov FU U.S. Environmental Protection Agency (EPA) [EP-D-13-013] FX Cell culture, chemical exposure, and HCI imaging were conducted by Apredica, Watertown, Massachusetts, under U.S. Environmental Protection Agency (EPA) contract EP-D-13-013. NR 28 TC 3 Z9 3 U1 3 U2 4 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD JUL PY 2016 VL 124 IS 7 BP 910 EP 919 DI 10.1289/ehp.1409029 PG 10 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DS4KF UT WOS:000380749300011 PM 26473631 ER PT J AU Mutlu, E Warren, SH Ebersviller, SM Kooter, IM Schmid, JE Dye, JA Linak, WP Gilmour, MI Jetter, JJ Higuchi, M DeMarini, DM AF Mutlu, Esra Warren, Sarah H. Ebersviller, Seth M. Kooter, Ingeborg M. Schmid, Judith E. Dye, Janice A. Linak, William P. Gilmour, M. Ian Jetter, James J. Higuchi, Mark DeMarini, David M. TI Mutagenicity and Pollutant Emission Factors of Solid-Fuel Cookstoves: Comparison with Other Combustion Sources SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID INDOOR AIR-POLLUTION; POLYCYCLIC AROMATIC-HYDROCARBONS; BIOASSAY-DIRECTED FRACTIONATION; COMPARATIVE RISK-ASSESSMENT; DIESEL EXHAUST PARTICLES; WOOD SMOKE EXPOSURE; CHEMICAL-ANALYSIS; SALMONELLA MUTAGENICITY; BIOMASS COOKSTOVES; CARBON-MONOXIDE AB BACKGROUND: Emissions from solid fuels used for cooking cause similar to 4 million premature deaths per year. Advanced solid-fuel cookstoves are a potential solution, but they should be assessed by appropriate performance indicators, including biological effects. OBJECTIVE: We evaluated two categories of solid-fuel cookstoves for eight pollutant and four mutagenicity emission factors, correlated the mutagenicity emission factors, and compared them to those of other combustion emissions. METHODS: We burned red oak in a 3-stone fire (TSF), a natural-draft stove (NDS), and a forceddraft stove (FDS), and we combusted propane as a liquified petroleum gas control fuel. We determined emission factors based on useful energy (megajoules delivered, MJd) for carbon monoxide, nitrogen oxides (NOx), black carbon, methane, total hydrocarbons, 32 polycyclic aromatic-hydrocarbons, PM2.5, levoglucosan (a wood-smoke marker), and mutagenicity in Salmonella. RESULTS: With the exception of NOx, the emission factors per MJ(d) were highly correlated (r >= 0.97); the correlation for NOx with the other emission factors was 0.58-0.76. Excluding NOx, the NDS and FDS reduced the emission factors an average of 68 and 92%, respectively, relative to the TSF. Nevertheless, the mutagenicity emission factor based on fuel energy used (MJ(thermal)) for the most efficient stove (FDS) was between those of a large diesel bus engine and a small diesel generator. CONCLUSIONS: Both mutagenicity and pollutant emission factors may be informative for characterizing cookstove performance. However, mutagenicity emission factors may be especially useful for characterizing potential health effects and should be evaluated in relation to health outcomes in future research. An FDS operated as intended by the manufacturer is safer than a TSF, but without adequate ventilation, it will still result in poor indoor air quality. C1 [Mutlu, Esra; Warren, Sarah H.; Schmid, Judith E.; Dye, Janice A.; Gilmour, M. Ian; Higuchi, Mark; DeMarini, David M.] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Mutlu, Esra] Univ N Carolina, Ctr Environm Med Asthma & Lung Biol, Chapel Hill, NC USA. [Ebersviller, Seth M.; Linak, William P.; Jetter, James J.] US EPA, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Kooter, Ingeborg M.] Netherlands Org Appl Sci Res TNO, Dept Environm Modelling Sensing & Anal, Utrecht, Netherlands. RP DeMarini, DM (reprint author), US EPA, B105-03, Res Triangle Pk, NC 27711 USA. EM demarini.david@epa.gov FU intramural research program of the Office of Research and Development of the U.S. Environmental Protection Agency (EPA), Research Triangle Park, NC FX Funding for this research was provided by the intramural research program of the Office of Research and Development of the U.S. Environmental Protection Agency (EPA), Research Triangle Park, NC. NR 54 TC 2 Z9 2 U1 15 U2 19 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD JUL PY 2016 VL 124 IS 7 BP 974 EP 982 DI 10.1289/ehp.1509852 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DS4KF UT WOS:000380749300019 PM 26895221 ER PT J AU Mansouri, K Abdelaziz, A Rybacka, A Roncaglioni, A Tropsha, A Varnek, A Zakharov, A Worth, A Richard, AM Grulke, CM Trisciuzzi, D Fourches, D Horvath, D Benfenati, E Muratov, E Wedebye, EB Grisoni, F Mangiatordi, GF Incisivo, GM Hong, HX Ng, HW Tetko, IV Balabin, I Kancherla, J Shen, J Burton, J Nicklaus, M Cassotti, M Nikolov, NG Nicolotti, O Andersson, PL Zang, QD Politi, R Beger, RD Todeschini, R Huang, RL Farag, S Rosenberg, SA Slavov, S Hu, X Judson, RS AF Mansouri, Kamel Abdelaziz, Ahmed Rybacka, Aleksandra Roncaglioni, Alessandra Tropsha, Alexander Varnek, Alexandre Zakharov, Alexey Worth, Andrew Richard, Ann M. Grulke, Christopher M. Trisciuzzi, Daniela Fourches, Denis Horvath, Dragos Benfenati, Emilio Muratov, Eugene Wedebye, Eva Bay Grisoni, Francesca Mangiatordi, Giuseppe F. Incisivo, Giuseppina M. Hong, Huixiao Ng, Hui W. Tetko, Igor V. Balabin, Ilya Kancherla, Jayaram Shen, Jie Burton, Julien Nicklaus, Marc Cassotti, Matteo Nikolov, Nikolai G. Nicolotti, Orazio Andersson, Patrik L. Zang, Qingda Politi, Regina Beger, Richard D. Todeschini, Roberto Huang, Ruili Farag, Sherif Rosenberg, Sine A. Slavov, Svetoslav Hu, Xin Judson, Richard S. TI CERAPP: Collaborative Estrogen Receptor Activity Prediction Project SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID ENDOCRINE-DISRUPTING CHEMICALS; COMPUTATIONAL TOXICOLOGY RESOURCE; QUANTITATIVE STRUCTURE-ACTIVITY; THROUGHPUT SCREENING ASSAYS; RELATIONSHIP QSDAR MODELS; ENVIRONMENTAL CHEMICALS; DECISION FOREST; PARTITION-COEFFICIENTS; BINARY CLASSIFICATION; BIOLOGICAL-ACTIVITY AB BACKGROUND: Humans are exposed to thousands of man-made chemicals in the environment. Some chemicals mimic natural endocrine hormones and, thus, have the potential to be endocrine disruptors. Most of these chemicals have never been tested for their ability to interact with the estrogen receptor (ER). Risk assessors need tools to prioritize chemicals for evaluation in costly in vivo tests, for instance, within the U.S. EPA Endocrine Disruptor Screening Program. OBJECTIVES: We describe a large-scale modeling project called CERAPP (Collaborative Estrogen Receptor Activity Prediction Project) and demonstrate the efficacy of using predictive computational models trained on high-throughput screening data to evaluate thousands of chemicals for ER-related activity and prioritize them for further testing. METHODS: CERAPP combined multiple models developed in collaboration with 17 groups in the United States and Europe to predict ER activity of a common set of 32,464 chemical structures. Quantitative structure-activity relationship models and docking approaches were employed, mostly using a common training set of 1,677 chemical structures provided by the U.S. EPA, to build a total of 40 categorical and 8 continuous models for binding, agonist, and antagonist ER activity. All predictions were evaluated on a set of 7,522 chemicals curated from the literature. To overcome the limitations of single models, a consensus was built by weighting models on scores based on their evaluated accuracies. RESULTS: Individual model scores ranged from 0.69 to 0.85, showing high prediction reliabilities. Out of the 32,464 chemicals, the consensus model predicted 4,001 chemicals (12.3%) as high priority actives and 6,742 potential actives (20.8%) to be considered for further testing. CONCLUSION: This project demonstrated the possibility to screen large libraries of chemicals using a consensus of different in silico approaches. This concept will be applied in future projects related to other end points. C1 [Mansouri, Kamel; Richard, Ann M.; Grulke, Christopher M.; Kancherla, Jayaram; Judson, Richard S.] US EPA, Natl Ctr Computat Toxicol, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Mansouri, Kamel] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Abdelaziz, Ahmed; Tetko, Igor V.] German Res Ctr Environm Hlth GmbH, Helmholtz Zentrum Muenchen, Inst Struct Biol, Neuherberg, Germany. [Rybacka, Aleksandra; Andersson, Patrik L.] Umea Univ, Dept Chem, Umea, Sweden. [Roncaglioni, Alessandra; Benfenati, Emilio; Incisivo, Giuseppina M.] Ist Ric Farmacol Mario Negri, IRCCS, Environm Chem & Toxicol Lab, Milan, Italy. [Tropsha, Alexander; Fourches, Denis; Muratov, Eugene; Politi, Regina; Farag, Sherif] Univ North Carolina Chapel Hill, Lab Mol Modeling, Chapel Hill, NC USA. [Varnek, Alexandre; Horvath, Dragos] Univ Strasbourg, Lab Chemoinformat, Strasbourg, France. [Zakharov, Alexey; Nicklaus, Marc] NCI, NIH, US Dept HHS, Bethesda, MD 20892 USA. [Worth, Andrew; Burton, Julien] European Commiss Ispra, Joint Res Ctr, IHCP, Ispra, Italy. [Trisciuzzi, Daniela; Mangiatordi, Giuseppe F.; Nicolotti, Orazio] Univ Bari, Dept Pharmacy Drug Sci, Bari, Italy. [Wedebye, Eva Bay; Nikolov, Nikolai G.; Rosenberg, Sine A.] Tech Univ Denmark, Natl Food Inst, Div Toxicol & Risk Assessment, Copenhagen, Denmark. [Grisoni, Francesca; Cassotti, Matteo; Todeschini, Roberto] Univ Milano Bicocca, Milano Chemometr & QSAR Res Grp, Milan, Italy. [Hong, Huixiao; Ng, Hui W.] US FDA, Div Bioinformat & Biostat, Natl Ctr Toxicol Res, USDA, Jefferson, AZ USA. [Tetko, Igor V.] BigChem GmbH, Neuherberg, Germany. [Balabin, Ilya] Lockheed Martin, High Performance Comp, Res Triangle Pk, NC USA. [Shen, Jie] Res Inst Fragrance Mat Inc, Woodcliff Lake, NJ USA. [Zang, Qingda; Slavov, Svetoslav] Integrated Lab Syst Inc, Res Triangle Pk, NC USA. [Beger, Richard D.] USDA, Div Syst Biol, Natl Ctr Toxicol Res, Jefferson, AZ USA. [Huang, Ruili; Hu, Xin] NIH, Natl Ctr Adv Translat Sci, DHHS, Bldg 10, Bethesda, MD 20892 USA. RP Judson, RS (reprint author), US EPA, Natl Ctr Computat Toxicol, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM judson.richard@epa.gov RI Tetko, Igor/B-1540-2010; Varnek, Alexandre/E-7076-2017; Kancherla, Jayaram/P-7756-2015; OI Tetko, Igor/0000-0002-6855-0012; Varnek, Alexandre/0000-0003-1886-925X; Kancherla, Jayaram/0000-0001-5855-5031; Mansouri, Kamel/0000-0002-6426-8036 FU NIGMS NIH HHS [T32 GM067553] NR 101 TC 17 Z9 17 U1 14 U2 15 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD JUL PY 2016 VL 124 IS 7 BP 1023 EP 1033 DI 10.1289/ehp.1510267 PG 11 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DS4KF UT WOS:000380749300025 PM 26908244 ER PT J AU Leung, MCK Phuong, J Baker, NC Sipes, NS Klinefelter, GR Martin, MT McLaurin, KW Setzer, RW Darney, SP Judson, RS Knudsen, TB AF Leung, Maxwell C. K. Phuong, Jimmy Baker, Nancy C. Sipes, Nisha S. Klinefelter, Gary R. Martin, Matthew T. McLaurin, Keith W. Setzer, R. Woodrow Darney, Sally Perreault Judson, Richard S. Knudsen, Thomas B. TI Systems Toxicology of Male Reproductive Development: Profiling 774 Chemicals for Molecular Targets and Adverse Outcomes SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID TESTICULAR DYSGENESIS SYNDROME; INTERLEUKIN-6 SIGNAL BLOCKADE; GERM-CELL DIFFERENTIATION; RECEPTOR TYROSINE KINASE; GENOME-WIDE ASSOCIATION; LARGE GENE LISTS; FETAL TESTIS; DI(N-BUTYL) PHTHALATE; NUCLEAR RECEPTORS; DIETHYLHEXYL PHTHALATE AB BACKGROUND: Trends in male reproductive health have been reported for increased rates of testicular germ cell tumors, low semen quality, cryptorchidism, and hypospadias, which have been associated with prenatal environmental chemical exposure based on human and animal studies. OBJECTIVE: In the present study we aimed to identify significant correlations between environmental chemicals, molecular targets, and adverse outcomes across a broad chemical landscape with emphasis on developmental toxicity of the male reproductive system. METHODS: We used U.S. EPA's animal study database (ToxRefDB) and a comprehensive literature analysis to identify 774 chemicals that have been evaluated for adverse effects on male reproductive parameters, and then used U.S. EPA's in vitro high-throughput screening (HTS) database (ToxCastDB) to profile their bioactivity across approximately 800 molecular and cellular features. RESULTS: A phenotypic hierarchy of testicular atrophy, sperm effects, tumors, and malformations, a composite resembling the human testicular dysgenesis syndrome (TDS) hypothesis, was observed in 281 chemicals. A subset of 54 chemicals with male developmental consequences had in vitro bioactivity on molecular targets that could be condensed into 156 gene annotations in a bipartite network. CONCLUSION: Computational modeling of available in vivo and in vitro data for chemicals that produce adverse effects on male reproductive end points revealed a phenotypic hierarchy across animal studies consistent with the human TDS hypothesis. We confirmed the known role of estrogen and androgen signaling pathways in rodent TDS, and importantly, broadened the list of molecular targets to include retinoic acid signaling, vascular remodeling proteins, G-protein coupled receptors (GPCRs), and cytochrome P450s. C1 [Leung, Maxwell C. K.; Sipes, Nisha S.; McLaurin, Keith W.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Leung, Maxwell C. K.; Phuong, Jimmy; Sipes, Nisha S.; Martin, Matthew T.; McLaurin, Keith W.; Setzer, R. Woodrow; Judson, Richard S.; Knudsen, Thomas B.] US EPA, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. [Baker, Nancy C.] Lockheed Martin, Res Triangle Pk, NC USA. [Klinefelter, Gary R.; Darney, Sally Perreault] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Darney, Sally Perreault] NIEHS, Environm Hlth Perspect, NIH, US Dept HHS, POB 12233, Res Triangle Pk, NC 27709 USA. RP Leung, MCK; Knudsen, TB (reprint author), US EPA, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM leung.max-well@epa.gov; knudsen.thomas@epa.gov NR 98 TC 2 Z9 2 U1 12 U2 12 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD JUL PY 2016 VL 124 IS 7 BP 1050 EP 1061 DI 10.1289/ehp.1510385 PG 12 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DS4KF UT WOS:000380749300028 PM 26662846 ER PT J AU Ginsberg, G Ginsberg, J Foos, B AF Ginsberg, Gary Ginsberg, Justine Foos, Brenda TI Approaches to Children's Exposure Assessment: Case Study with Diethylhexylphthalate (DEHP) SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Review DE children; pregnancy; breast milk; house dust; contaminants; exposure assessment; plasticizer; phthalate; DEHP ID POLYBROMINATED DIPHENYL ETHERS; CUMULATIVE RISK-ASSESSMENT; IN-UTERO EXPOSURE; PHTHALATE EXPOSURE; DI(2-ETHYLHEXYL) PHTHALATE; ENVIRONMENTAL TOXICANTS; GENERAL-POPULATION; BIOMONITORING DATA; DIETARY EXPOSURE; BISPHENOL-A AB Children's exposure assessment is a key input into epidemiology studies, risk assessment and source apportionment. The goals of this article are to describe a methodology for children's exposure assessment that can be used for these purposes and to apply the methodology to source apportionment for the case study chemical, diethylhexylphthalate (DEHP). A key feature is the comparison of total (aggregate) exposure calculated via a pathways approach to that derived from a biomonitoring approach. The 4-step methodology and its results for DEHP are: (1) Prioritization of life stages and exposure pathways, with pregnancy, breast-fed infants, and toddlers the focus of the case study and pathways selected that are relevant to these groups; (2) Estimation of pathway-specific exposures by life stage wherein diet was found to be the largest contributor for pregnant women, breast milk and mouthing behavior for the nursing infant and diet, house dust, and mouthing for toddlers; (3) Comparison of aggregate exposure by pathways vs biomonitoring-based approaches wherein good concordance was found for toddlers and pregnant women providing confidence in the exposure assessment; (4) Source apportionment in which DEHP presence in foods, children's products, consumer products and the built environment are discussed with respect to early life mouthing, house dust and dietary exposure. A potential fifth step of the method involves the calculation of exposure doses for risk assessment which is described but outside the scope for the current case study. In summary, the methodology has been used to synthesize the available information to identify key sources of early life exposure to DEHP. C1 [Ginsberg, Gary; Ginsberg, Justine] Partnership Pediat & Environm Hlth, Granby, CT 06026 USA. [Foos, Brenda] US EPA, Off Childrens Hlth Protect, Washington, DC 20460 USA. RP Ginsberg, G (reprint author), Partnership Pediat & Environm Hlth, Granby, CT 06026 USA. EM hologram7942@yahoo.com; justine1973@hotmail.com; foos.brenda@epa.gov NR 77 TC 2 Z9 2 U1 6 U2 7 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD JUL PY 2016 VL 13 IS 7 AR 670 DI 10.3390/ijerph13070670 PG 21 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DS4OG UT WOS:000380759800046 ER PT J AU Zaleski, RT Egeghy, PP Hakkinen, PJ AF Zaleski, Rosemary T. Egeghy, Peter P. Hakkinen, Pertti J. TI Exploring Global Exposure Factors Resources for Use in Consumer Exposure Assessments SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Review DE consumer products; exposure factors; databases; exposure assessments; online; time-activity patterns; consumer behavior; household products ID PERSONAL CARE PRODUCTS; NONDIETARY INGESTION EXPOSURE; MOUTH FREQUENCY DATA; FACTORS HANDBOOK; COSMETIC PRODUCTS; RISK-ASSESSMENT; 21ST-CENTURY ROADMAP; YOUNG-CHILDREN; INDOOR AIR; CHEMICALS AB This publication serves as a global comprehensive resource for readers seeking exposure factor data and information relevant to consumer exposure assessment. It describes the types of information that may be found in various official surveys and online and published resources. The relevant exposure factors cover a broad range, including general exposure factor data found in published compendia and databases and resources about specific exposure factors, such as human activity patterns and housing information. Also included are resources on exposure factors related to specific types of consumer products and the associated patterns of use, such as for a type of personal care product or a type of children's toy. Further, a section on using exposure factors for designing representative exposure scenarios is included, along with a look into the future for databases and other exposure science developments relevant for consumer exposure assessment. C1 [Zaleski, Rosemary T.] ExxonMobil Biomed Sci Inc, 1545 Route 22 East, Annandale, NJ 08801 USA. [Egeghy, Peter P.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Hakkinen, Pertti J.] Natl Lib Med, NIH, 6707 Democracy Blvd,Suite 510, Bethesda, MD 20894 USA. RP Zaleski, RT (reprint author), ExxonMobil Biomed Sci Inc, 1545 Route 22 East, Annandale, NJ 08801 USA. EM rosemary.t.zaleski@exxonmobil.com; egeghy.peter@epa.gov; pertti.hakkinen@nih.gov FU ExxonMobil Biomedical Sciences, Inc. FX The International Society of Exposure Science Symposium on Exploring Global Data Resources for Consumer Exposure Assessment served both as the inspiration for developing this publication, as well as the basis for much of the information provided in the document (costs of open access were paid for by ExxonMobil Biomedical Sciences, Inc.). The authors (symposium co-organizers) would like to thank all of the conference presenters for the information shared both at the symposium and for inclusion in this paper, as well as help in manuscript review, by recognizing them as contributors below. NR 139 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD JUL PY 2016 VL 13 IS 7 AR 744 DI 10.3390/ijerph13070744 PG 26 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DS4OG UT WOS:000380759800120 ER PT J AU Spring, SR Bastian, TW Wang, Y Kosian, P Anderson, GW Gilbert, ME AF Spring, S. R. Bastian, T. W. Wang, Y. Kosian, P. Anderson, G. W. Gilbert, M. E. TI Thyroid hormone-dependent formation of a subcortical band heterotopia (SBH) in the neonatal brain is not exacerbated under conditions of low dietary iron (FeD) SO NEUROTOXICOLOGY AND TERATOLOGY LA English DT Article DE Developmental hypothyroidism; Subcortical band heterotopia; Propylthiouracil; Iron deficiency; FeD ID CEREBRAL-CORTEX; DEFICIENCY; FETAL; INSUFFICIENCY; HIPPOCAMPUS; MODEL AB Thyroid hormones (TH) are critical for brain development and insufficiencies can lead to structural abnormalities in specific brain regions. Administration of the goitrogen propylthiouracil (PTU) reduces TH production by inhibiting thyroperoxidase (TPO), an enzyme that oxidizes iodide for the synthesis of TH. TPO activity is iron (Fe)-dependent and dietary iron deficiency (FeD) also reduces circulating levels of TH. We have previously shown that modest degrees of THinsufficiency induced in pregnant rat dams alters the expression of TH-responsive genes in the cortex and hippocampus of the neonate, and results in the formation of a subcortical band heterotopia (SBH) in the corpus callosum (Royland et al., 2008, Bastian et al., 2014, Gilbert et al., 2014). The present experiment investigated if FeD alone was sufficient to induce a SBH or if FeD would augment SBH formation at lower doses of PTU. One set of pregnant rats was administered 0, 1, 3, or 10 ppm of PTU via drinking water starting on gestational day (GD) 6. FeD was induced in a 2nd set of dams beginning on GD2. A third set of dams received the FeD diet from GD2 paired with either 1 ppm or 3 ppm PTU beginning on GD6. All treatments continued until the time of sacrifice. On PN18, one female pup from each litter was sacrificed' and the brain examined for SBH. We observed lower maternal, PN2 and PN18 pup serum T4 in response to PTU. FeD reduced serum T4 in pups on PN16, but did not affect serum T4 in dams or PN2 pups. Neither did FeD in combination with PTU alter T4 levels in dams on PN18 or pups on PN2 compared to PTU treatment alone. By PN16, however more severe T4 reductions were observed in pups when FeD was combined with PTU. SBH increased with increasing dosage of PTU, but counter to our hypothesis, no SBH was detected in the offspring of FeD dams. As such, T4 levels in dams and newborn pups rather than older neonates appear to be a better predictor SBH associated with TH insufficiency. These data indirectly support previous work indicating prenatal TH insufficiency but not postnatal TH insufficiency in offspring is required for SBH formation. Published by Elsevier Inc. C1 [Spring, S. R.; Gilbert, M. E.] US EPA, Tox Assessment Div, Natl Hlth & Environm Effects Res Lab, MD B105-05, Res Triangle Pk, NC 27711 USA. [Bastian, T. W.; Wang, Y.; Anderson, G. W.] Univ Minnesota, Coll Pharm, Duluth, MN 55812 USA. [Kosian, P.] US EPA, Natl Hlth & Environm Effects Res Lab, MidContinent Ecol Div, Duluth, MN USA. RP Gilbert, ME (reprint author), US EPA, Tox Assessment Div, Natl Hlth & Environm Effects Res Lab, MD B105-05, Res Triangle Pk, NC 27711 USA. EM gilbert.mary@epa.gov FU NIEHS [5R03HD055423-02]; University of Minnesota Doctoral Dissertation Fellowship FX This work was supported by NIEHS Grant 5R03HD055423-02 to GA and University of Minnesota Doctoral Dissertation Fellowship to TB. This document has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. The authors also thank Drs. Raddy Ramos and Sigmund Degitz for their comments on an earlier version of this manuscript, and Mr. Luke Ford for technical assistance. NR 16 TC 0 Z9 0 U1 2 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0892-0362 EI 1872-9738 J9 NEUROTOXICOL TERATOL JI Neurotoxicol. Teratol. PD JUL-AUG PY 2016 VL 56 SI SI BP 41 EP 46 DI 10.1016/j.ntt.2016.05.007 PG 6 WC Neurosciences; Toxicology SC Neurosciences & Neurology; Toxicology GA DS2QF UT WOS:000380627900006 PM 27216871 ER PT J AU Calle, L Gawlik, DE Xie, ZX Green, L Lapointe, B Strong, A AF Calle, Leonardo Gawlik, Dale E. Xie, Zhixiao Green, Lauri Lapointe, Brian Strong, Allan TI Effects of tidal periodicities and diurnal foraging constraints on the density of foraging wading birds SO AUK LA English DT Article DE Egretta caerulea; foraging ecology; intertidal zone; moon phase; tidal model; tides; TiMSA; wading birds ID SHALLOW-WATER HABITATS; GREAT BLUE HERONS; SALT-MARSH; NEKTON USE; BREEDING-SEASON; FEEDING HABITAT; MARINE ANIMALS; ARDEA-CINEREA; CYCLE; SHOREBIRDS AB In intertidal zones, tidal cycles reduce water depths and provide areas of shallow water where wading birds can forage for aquatic prey (water depths 0-50 cm). However, a bird that forages diurnally can make use of only a portion of the tidal cycle, which can limit fulfillment of energetic demands. Furthermore, daily and biweekly (spring-neap) tides may compound effects on shallow-water availability for foraging birds. However, the relative effects of daily and biweekly tidal periodicities on the foraging ecology of wading birds are seldom investigated due to a lack of appropriate tools. Therefore, we developed a tidal simulation model to provide dynamic spatiotemporal estimates of the availability of water depths that are within the upper and lower bounds of the birds' foraging water depth limits('' shallow-water availability ''). We studied two wading bird species, the Little Blue Heron (Egretta caerulea), a daytime-only forager, and the Great White Heron (Ardea herodias occidentalis), which feeds both diurnally and nocturnally, to evaluate the relative effects of daily and biweekly tides on shallow-water availability and on patterns in abundance of foraging birds. Seasonal foraging surveys (n = 38; 2011-2013) were conducted by boat along a 14-km transect adjacent to extensive intertidal flats in the lower Florida Keys, USA. For both species combined, biweekly tides resulted in a 0.61- to 6.09-fold change in abundance, whereas daily tides resulted in a 1.03- to 5.81-fold change in abundance. Diurnal shallow-water availability was not consistently correlated in magnitude or direction with spring-neap tidal cycles because differences in tide height between consecutive low tides were larger than changes in tidal amplitude from spring-neap tide cycles. Thus, the strong response by birds to the spring-neap tide was likely driven by mechanisms other than diurnal shallow-water availability alone. C1 [Calle, Leonardo; Gawlik, Dale E.] Florida Atlantic Univ, Environm Sci Program, Boca Raton, FL 33431 USA. [Xie, Zhixiao] Florida Atlantic Univ, Dept Geosci, Boca Raton, FL 33431 USA. [Green, Lauri] US EPA, Newport, OR USA. [Lapointe, Brian] Florida Atlantic Univ, Harbor Branch Oceanog Inst, Ft Pierce, FL USA. [Strong, Allan] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT USA. [Calle, Leonardo] Montana State Univ, Dept Ecol, Ecosyst Dynam Lab, Bozeman, MT 59717 USA. RP Calle, L (reprint author), Florida Atlantic Univ, Environm Sci Program, Boca Raton, FL 33431 USA.; Calle, L (reprint author), Montana State Univ, Dept Ecol, Ecosyst Dynam Lab, Bozeman, MT 59717 USA. EM leonardo.calle@msu.montana.edu FU U.S. Fish and Wildlife Service [40181AG098, F12AC01244] FX Funding: This study was financially supported by U.S. Fish and Wildlife Service awards #40181AG098 and #F12AC01244. None of the funders had any influence on the content of the submitted or published manuscript. None of the funders require approval of the final manuscript to be published. NR 59 TC 0 Z9 0 U1 16 U2 22 PU AMER ORNITHOLOGISTS UNION PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0004-8038 EI 1938-4254 J9 AUK JI AUK PD JUL PY 2016 VL 133 IS 3 BP 378 EP 396 DI 10.1642/AUK-15-234.1 PG 19 WC Ornithology SC Zoology GA DR1KC UT WOS:000379663400005 ER PT J AU Bancroft, BA Lawler, JJ Schumaker, NH AF Bancroft, Betsy A. Lawler, Joshua J. Schumaker, Nathan H. TI Weighing the relative potential impacts of climate change and land-use change on an endangered bird SO ECOLOGY AND EVOLUTION LA English DT Article DE Development; environmental stress; habitat change; habitat loss; HexSim; Picoides borealis; population model; precipitation; Red-cockaded Woodpecker; spatially explicit individual-based model ID RED-COCKADED WOODPECKERS; HABITAT LOSS; EXTINCTION RISK; FOREST; MODEL; SIZE; FRAGMENTATION; BIODIVERSITY; LANDSCAPES; ECOSYSTEMS AB Climate change and land-use change are projected to be the two greatest drivers of biodiversity loss over the coming century. Land-use change has resulted in extensive habitat loss for many species. Likewise, climate change has affected many species resulting in range shifts, changes in phenology, and altered interactions. We used a spatially explicit, individual-based model to explore the effects of land-use change and climate change on a population of the endangered Red-cockaded Woodpecker (RCW; Picoides borealis). We modeled the effects of land-use change using multiple scenarios representing different spatial arrangements of new training areas for troops across Fort Benning. We used projected climate-driven changes in habitat and changes in reproductive output to explore the potential effects of climate change. We summarized potential changes in habitat based on the output of the dynamic vegetation model LPJ-GUESS, run for multiple climate change scenarios through the year 2100. We projected potential changes in reproduction based on an empirical relationship between spring precipitation and the mean number of successful fledglings produced per nest attempt. As modeled in our study, climate change had virtually no effect on the RCW population. Conversely, simulated effects of land-use change resulted in the loss of up to 28 breeding pairs by 2100. However, the simulated impacts of development depended on where the development occurred and could be completely avoided if the new training areas were placed in poor-quality habitat. Our results demonstrate the flexibility inherent in many systems that allows seemingly incompatible human land uses, such as development, and conservation actions to exist side by side. C1 [Bancroft, Betsy A.; Lawler, Joshua J.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. US EPA, Western Div, Corvallis, OR USA. [Bancroft, Betsy A.] Gonzaga Univ, Dept Biol, 502 E Boone Ave, Spokane, WA 99258 USA. [Bancroft, Betsy A.] Gonzaga Univ, Dept Environm Studies, 502 E Boone Ave, Spokane, WA 99258 USA. RP Bancroft, BA (reprint author), Gonzaga Univ, Dept Biol, 502 E Boone Ave, Spokane, WA 99258 USA. EM bancroft@gonzaga.edu FU US Environmental Protection Agency, Strategic Environmental Research and Development Program [RC-1541] FX US Environmental Protection Agency, Strategic Environmental Research and Development Program (Grant/Award Number: RC-1541) NR 52 TC 0 Z9 0 U1 22 U2 42 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD JUL PY 2016 VL 6 IS 13 BP 4468 EP 4477 DI 10.1002/ece3.2204 PG 10 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DQ6VU UT WOS:000379344400019 PM 27386089 ER PT J AU Rochman, CM Cook, AM Koelmans, AA AF Rochman, Chelsea M. Cook, Anna-Marie Koelmans, Albert A. TI Plastic debris and policy: Using current scientific understanding to invoke positive change SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Marine plastic; Microplastic; Policy; Hazard; risk assessment; Weight of evidence ID MICROPLASTIC POLLUTION; TECHNOLOGY-ASSESSMENT; GREAT-LAKES; MARINE; ACCUMULATION; SHORELINES; POLLUTANTS; INGESTION; SEDIMENTS; LITTER AB Captain Charles Moore introduced the world to the Great Pacific Garbage Patch in the mid-1990s, and images of plastic debris in the oceans began to sweep the media. Since then, there has been increasing interest from scientists, the public, and policy makers regarding plastic debris in the environment. Today, there remains no doubt that plastic debris contaminates aquatic (marine and freshwater) habitats and animals globally. The growing scientific evidence demonstrates widespread contamination from plastic debris, and researchers are beginning to understand the sources, fate, and effects of the material. As new scientific understanding breeds new questions, scientists are working to fill data gaps regarding the fate and effects of plastic debris and the mechanisms that drive these processes. In parallel, policy makers are working to mitigate this contamination. The authors focus on what is known about plastic debris that is relevant to policy by reviewing some of the weight of evidence regarding contamination, fate, and effects of the material. Moreover, they highlight some examples of how science has already been used to inform policy change and mitigation and discuss opportunities for future linkages between science and policy to continue the relationship and contribute to effective solutions for plastic debris. Environ Toxicol Chem 2016;35:1617-1626. (c) 2016 SETAC C1 [Rochman, Chelsea M.] Soc Conservat Biol, David H Smith Conservat Res Program, Washington, DC USA. [Rochman, Chelsea M.] Univ Calif Davis, Sch Vet Med, Dept Anat Physiol & Cellular Biol, Davis, CA 95616 USA. [Cook, Anna-Marie] US EPA, Reg 9, San Francisco, CA USA. [Koelmans, Albert A.] Wageningen Univ, Dept Environm Sci, Aquat Ecol & Water Qual Management Grp, Wageningen, Netherlands. [Koelmans, Albert A.] Wageningen UR, IMARES, Ijmuiden, Netherlands. RP Rochman, CM (reprint author), Soc Conservat Biol, David H Smith Conservat Res Program, Washington, DC USA.; Rochman, CM (reprint author), Univ Calif Davis, Sch Vet Med, Dept Anat Physiol & Cellular Biol, Davis, CA 95616 USA. EM cmrochman@ucdavis.edu FU David H. Smith Postdoctoral Research Fellowship FX We thank A. Burton for advising us on preparation and reviewing drafts of the manuscript. C. M. Rochman was funded by a David H. Smith Postdoctoral Research Fellowship during preparation of the manuscript. All authors declare no conflict of interest. NR 64 TC 3 Z9 3 U1 118 U2 170 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD JUL PY 2016 VL 35 IS 7 BP 1617 EP 1626 DI 10.1002/etc.3408 PG 10 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DQ9RV UT WOS:000379547900003 PM 27331654 ER PT J AU Munns, WR Rea, AW Suter, GW Martin, L Blake-Hedges, L Crk, T Davis, C Ferreira, G Jordan, S Mahoney, M Barron, MG AF Munns, Wayne R., Jr. Rea, Anne W. Suter, Glenn W., II Martin, Lawrence Blake-Hedges, Lynne Crk, Tanja Davis, Christine Ferreira, Gina Jordan, Steve Mahoney, Michele Barron, Mace G. TI Ecosystem services as assessment endpoints for ecological risk assessment SO INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT LA English DT Article DE Assessment endpoints; Ecological risk assessment; Ecosystem services; Generic ecological assessment endpoints ID FRAMEWORK AB Ecosystem services are defined as the outputs of ecological processes that contribute to human welfare or have the potential to do so in the future. Those outputs include food and drinking water, clean air and water, and pollinated crops. The need to protect the services provided by natural systems has been recognized previously, but ecosystem services have not been formally incorporated into ecological risk assessment practice in a general way in the United States. Endpoints used conventionally in ecological risk assessment, derived directly from the state of the ecosystem (e.g., biophysical structure and processes), and endpoints based on ecosystem services serve different purposes. Conventional endpoints are ecologically important and susceptible entities and attributes that are protected under US laws and regulations. Ecosystem service endpoints are a conceptual and analytical step beyond conventional endpoints and are intended to complement conventional endpoints by linking and extending endpoints to goods and services with more obvious benefit to humans. Conventional endpoints can be related to ecosystem services even when the latter are not considered explicitly during problem formulation. To advance the use of ecosystem service endpoints in ecological risk assessment, the US Environmental Protection Agency's Risk Assessment Forum has added generic endpoints based on ecosystem services (ES-GEAE) to the original 2003 set of generic ecological assessment endpoints (GEAEs). Like conventional GEAEs, ES-GEAEs are defined by an entity and an attribute. Also like conventional GEAEs, ES-GEAEs are broadly described and will need to be made specific when applied to individual assessments. Adoption of ecosystem services as a type of assessment endpoint is intended to improve the value of risk assessment to environmental decision making, linking ecological risk to human well-being, and providing an improved means of communicating those risks. Integr Environ Assess Manag 2016;12:522-528. Published 2015 SETAC. This article is a US Government work and, as such, is in the public domain in the USA. C1 [Munns, Wayne R., Jr.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Narragansett, RI 02882 USA. [Rea, Anne W.] US EPA, Off Res & Dev, Safe & Sustainable Water Resources Res Program, Res Triangle Pk, NC 27711 USA. [Suter, Glenn W., II] US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Cincinnati, OH 45268 USA. [Martin, Lawrence] US EPA, Off Res & Dev, Off Sci Advisor, Washington, DC 20460 USA. [Blake-Hedges, Lynne] US EPA, Off Chem Safety & Pollut Prevent, Off Pollut Prevent & Tox, Washington, DC 20460 USA. [Crk, Tanja] US EPA, Off Chem Safety & Pollut Prevent, Off Pesticide Programs, Washington, DC 20460 USA. [Davis, Christine] US EPA, Off Air & Radiat, Off Air Qual Planning & Stand, Res Triangle Pk, NC 27711 USA. [Ferreira, Gina] US EPA, Reg 2, Clean Air & Sustainabil Div, New York, NY USA. [Jordan, Steve; Barron, Mace G.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Gulf Breeze, FL USA. [Mahoney, Michele] US EPA, Off Solid Waste & Emergency Response, Off Superfund Remediat & Technol Innovat, Washington, DC 20460 USA. RP Munns, WR (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Narragansett, RI 02882 USA. EM munns.wayne@epa.gov NR 40 TC 8 Z9 8 U1 6 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1551-3777 EI 1551-3793 J9 INTEGR ENVIRON ASSES JI Integr. Environ. Assess. Manag. PD JUL PY 2016 VL 12 IS 3 BP 522 EP 528 DI 10.1002/ieam.1707 PG 7 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DQ9SJ UT WOS:000379549300015 PM 26331725 ER PT J AU Snow, M Darveau, L Lowery, A DiBara, M AF Snow, Madeline Darveau, Linda Lowery, Ann DiBara, Michael TI Innovation in the Water Sector: Pathway to Zero Net Energy SO JOURNAL AMERICAN WATER WORKS ASSOCIATION LA English DT Article C1 [Snow, Madeline] Univ Massachusetts, Lowell Ctr Sustainable Prod, TURI, 600 Suffolk St,5th Fl, Lowell, MA 01854 USA. [Snow, Madeline] US EPA, Washington, DC 20460 USA. [Snow, Madeline; Lowery, Ann] Massachusetts Dept Environm Protect, Boston, MA USA. [Darveau, Linda] USEPA Reg 1 New England, Boston, MA USA. [DiBara, Michael] Massachusetts Dept Environm Protect, Worcester, MA USA. RP Snow, M (reprint author), Univ Massachusetts, Lowell Ctr Sustainable Prod, TURI, 600 Suffolk St,5th Fl, Lowell, MA 01854 USA. EM madeline_snow@uml.edu NR 4 TC 1 Z9 1 U1 1 U2 1 PU AMER WATER WORKS ASSOC PI DENVER PA 6666 W QUINCY AVE, DENVER, CO 80235 USA SN 2164-4535 J9 J AM WATER WORKS ASS JI J. Am. Water Work Assoc. PD JUL PY 2016 VL 108 IS 7 BP 78 EP 82 DI 10.5942/jawwa.2016.108.0116 PG 5 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA DQ7IV UT WOS:000379381300012 ER PT J AU Revetta, RP Gomez-Alvarez, V Gerke, TL Domingo, JW Ashbolt, NJ AF Revetta, R. P. Gomez-Alvarez, V. Gerke, T. L. Santo Domingo, J. W. Ashbolt, N. J. TI Changes in bacterial composition of biofilm in a metropolitan drinking water distribution system SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE biofilm; drinking water; drinking water distribution system; groundwater; microbial structure; surface water ID NONTUBERCULOUS MYCOBACTERIA; MICROBIAL COMMUNITIES; DIVERSITY; RNA; 16S; CHLORINE; NETWORK; PATHOGENS; DYNAMICS; ECOLOGY AB AimsThis study examined the development of bacterial biofilms within a metropolitan distribution system. The distribution system is fed with different source water (i.e. groundwater, GW and surface water, SW) and undergoes different treatment processes in separate facilities. Methods and ResultsThe biofilm community was characterized using 16S rRNA gene clone libraries and functional potential analysis, generated from total DNA extracted from coupons in biofilm annular reactors fed with onsite drinking water for up to 18months. Differences in the bacterial community structure were observed between GW and SW. Representatives that explained the dissimilarity were associated with the classes Betaproteobacteria, Alphaproteobacteria, Actinobacteria, Gammaproteobacteria and Firmicutes. After 9months the biofilm bacterial community from both GW and SW were dominated by Mycobacterium species. The distribution of the dominant operational taxonomic unit (OTU) (Mycobacterium) positively correlated with the drinking water distribution system (DWDS) temperature. ConclusionsIn this study, the biofilm community structure observed between GW and SW were dissimilar, while communities from different locations receiving SW did not show significant differences. The results suggest that source water and/or the water quality shaped by their respective treatment processes may play an important role in shaping the bacterial communities in the distribution system. In addition, several bacterial groups were present in all samples, suggesting that they are an integral part of the core microbiota of this DWDS. Significance and Impact of the StudyThese results provide an ecological insight into biofilm bacterial structure in chlorine-treated drinking water influenced by different water sources and their respective treatment processes. C1 [Revetta, R. P.; Gomez-Alvarez, V.; Santo Domingo, J. W.; Ashbolt, N. J.] US EPA, Cincinnati, OH 45268 USA. [Gerke, T. L.] US EPA, ORISE, Cincinnati, OH 45268 USA. [Ashbolt, N. J.] Univ Alberta, Edmonton, AB, Canada. RP Revetta, RP (reprint author), ORD NRMRL WSWRD, MS681,26 West MLK Dr, Cincinnati, OH USA. EM revetta.randy@epa.gov FU US EPA through the Office of Research and Development FX The authors thank Jeff Swertfeger, David Hartman and Bill Fromme (Greater Cincinnati Water Works) for valuable discussions and suggestions during the planning process, and for assistance in obtaining water quality data and sample access. The US EPA through the Office of Research and Development funded and managed this research. The opinions expressed are those of the authors, and do not necessarily reflect the official positions and policies of the US EPA. Any mention of product or trade names does not constitute recommendation for use by the US EPA. NR 52 TC 1 Z9 1 U1 12 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD JUL PY 2016 VL 121 IS 1 BP 294 EP 305 DI 10.1111/jam.13150 PG 12 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DQ9NA UT WOS:000379535400026 PM 27037969 ER PT J AU Green, L Fong, P AF Green, Lauri Fong, Peggy TI The good, the bad and the Ulva: the density dependent role of macroalgal subsidies in influencing diversity and trophic structure of an estuarine community SO OIKOS LA English DT Article ID GREEN ALGAL MATS; CLIMATE-CHANGE; FOOD WEBS; MARINE-SEDIMENTS; COASTAL WATERS; PACIFIC SALMON; ECOSYSTEMS; DYNAMICS; SULFIDE; HABITAT AB Worldwide, ecological subsidies enhance ecosystem productivity and therefore trophic support for greater biodiversity of taxa. While studies in terrestrial and aquatic ecosystems demonstrate that the magnitude of subsidies into ecosystems differs widely, the thresholds where subsidies may switch from exerting positive to negative effects are poorly understood. In estuaries, eutrophication promotes drift macroalgae that deposit on the benthos, cover intertidal flats for months and serve as pressed resource subsidies for benthic consumers. We hypothesized there would be a critical threshold of macroalgal biomass where ecosystem-level effects would turn from positive to negative. We used manipulative field experiments varying macroalgal mat thickness (0.5, 1.5 and 4 cm) over eight weeks and quantified effects on macrofauna on a lagoon mudflat in California. We documented that plots with mat depths of 0.5 and 1.5 cm had higher diversity by supporting both surface feeding and burrowing detritivores. Non-metric multidimensional scaling showed that the benthic community diverged with mat depth over the course of the experiment. After eight weeks, surface deposit feeders were associated mainly with 0.5 cm macroalgal subsidies, whereas subsurface deposit feeding capitellids were closely linked with 4 cm mats. Depth profiles of pore water sulfide concentration collected from 4 cm mats were 7622 +/- 5294 mu M, mean +/- SE, (mean of means across depth profiles), whereas 0.5 cm treatments resulted in sulfide concentrations that were 0.25% of the 4 cm treatments. This suggests that the mechanism of negative effects for elevated macroalgal subsidies was development of anoxic conditions promoting sulfide accumulation. Thus, our study was the first to find a critical threshold, or ecological tipping point, beyond which the effects of anthropogenically enhanced subsidies to estuarine mudflat communities switched from positive to negative and to describe the mechanism by which elevated subsides altered the abiotic environment and likely reduced ecosystem functioning. C1 [Green, Lauri; Fong, Peggy] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, 621 Charles E Young Dr South, Los Angeles, CA 90095 USA. [Green, Lauri] US EPA, 2111 SE Marine Sci Dr, Newport, OR 97365 USA. RP Green, L (reprint author), Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, 621 Charles E Young Dr South, Los Angeles, CA 90095 USA.; Green, L (reprint author), US EPA, 2111 SE Marine Sci Dr, Newport, OR 97365 USA. EM ruiz-green.lauri@epa.gov NR 95 TC 0 Z9 0 U1 22 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0030-1299 EI 1600-0706 J9 OIKOS JI Oikos PD JUL PY 2016 VL 125 IS 7 BP 988 EP 1000 DI 10.1111/oik.02860 PG 13 WC Ecology SC Environmental Sciences & Ecology GA DR0PO UT WOS:000379609600010 ER PT J AU Yuan, YP Hu, WH Li, GY AF Yuan, Yongping Hu, Wenhui Li, Guangyong TI Evaluation of Soil Erosion and Sediment Yield From Ridge Watersheds Leading to Guanica Bay, Puerto Rico, Using the Soil and Water Assessment Tool Model SO SOIL SCIENCE LA English DT Article DE Coffee farming; land use; Puerto Rico; ridge watersheds; sediment yield; soil erosion; SWAT ID SENSITIVITY-ANALYSIS; SWAT MODEL; CALIBRATION; UNCERTAINTY; SIMULATION; TRANSPORT; QUALITY; RUNOFF; IMPACT; RIVER AB Increased sediment loading to reservoirs and, ultimately, to Guanica Bay and reef areas is a significant concern in Puerto Rico. Sediment deposition has significantly reduced storage capacity of reservoirs, and sediment-attached contaminants can stress corals and negatively impact reef health. In this study, we examined sediment yield from an upper mountainous watershed, Yahuecas, contributing sediment to Lago Yahuecas reservoir and eventually Guanica Bay, Puerto Rico, to gain a better understanding on sediment loss. This watershed was chosen because it was the only watershed where runoff was monitored in Guanica Bay basin. The Soil and Water Assessment Tool was calibrated and validated using 4 1/2 years of flow data (07/1980 to 01/1985) from the Yahuecas watershed. Five and a half years of suspended sediment concentration data (04/2000 to 09/2005) from the adjacent Adjuntas watershed were used to calibrate sediment simulation of the model because no sediment data were available for Yahuecas. After calibration and validation, Soil and Water Assessment Tool was used to evaluate temporal-spatial soil erosion and sediment yield and assess factors that impact sediment yield. From 1975 to 2011, approximately 80% of annual sediment yield occurred during the two rainy seasons (February to May and August to November). Heavy rainfall, erodible soils, and steep mountain slopes were the primary causes of sediment yield in the Yahuecas watershed. Land use that reduces the protective forest canopy (like sun-grown coffee farming) can exacerbate soil loss. More sediment per hectare was lost from areas producing coffee than forested or grass-covered areas. Conversion of coffee farming practices from sungrown to shade-grown will reduce soil erosion and sediment yield. C1 [Yuan, Yongping] USEPA NERL, Landscape Ecol Branch, Div Environm Sci, Las Vegas, NV USA. [Hu, Wenhui] Lincoln Univ, Dept Agr & Environm Sci, Jefferson City, MO USA. [Hu, Wenhui] Lincoln Univ, Cooperat Res Program, Jefferson City, MO USA. [Li, Guangyong] China Agr Univ, Beijing, Peoples R China. RP Yuan, YP (reprint author), US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. EM yuan.yongping@epa.gov NR 44 TC 0 Z9 0 U1 30 U2 30 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD JUL PY 2016 VL 181 IS 7 BP 315 EP 325 DI 10.1097/SS.0000000000000166 PG 11 WC Soil Science SC Agriculture GA DQ8CX UT WOS:000379437800006 ER PT J AU Ernstoff, AS Fantke, P Csiszar, SA Henderson, AD Chung, S Jolliet, O AF Ernstoff, Alexi S. Fantke, Peter Csiszar, Susan A. Henderson, Andrew D. Chung, Susie Jolliet, Olivier TI Multi-pathway exposure modeling of chemicals in cosmetics with application to shampoo SO ENVIRONMENT INTERNATIONAL LA English DT Article DE Exposure; Life cycle assessment; High-throughput screening; Cosmetics; Personal care products ID PERSONAL CARE PRODUCTS; PREDICTING SKIN PERMEABILITY; CYCLE IMPACT ASSESSMENT; IN-VITRO BIOACTIVITY; CONSUMER EXPOSURE; RISK-ASSESSMENT; DERMAL ABSORPTION; FRAGRANCE INGREDIENTS; INTAKE FRACTION; PRIORITIZATION AB We present a novel multi-pathway, mass balance based, fate and exposure model compatible with life cycle and high-throughput screening.assessments of chemicals in cosmetic products. The exposures through product use as well as post-use emissions and environmental media were quantified based on the chemical mass originally applied via a product, multiplied by the product intake fractions (PiF, the fraction of a chemical in a product that is taken in by exposed persons) to yield intake rates. The average PiFs for the evaluated chemicals in shampoo ranged from 3 x 10(-4) up to 0.3 for rapidly absorbed ingredients. Average intake rates ranged between nano- and micrograms per kilogram bodyweight per day; the order of chemical prioritization was strongly affected by the ingredient concentration in shampoo. Dermal intake and inhalation (for 20% of the evaluated chemicals) during use dominated exposure, while the skin permeation coefficient dominated the estimated uncertainties. The fraction of chemical taken in by a shampoo user often exceeded, by orders of magnitude, the aggregated fraction taken in by the population through post-use environmental emissions. Chemicals with relatively high octanol-water partitioning and/or volatility, and low molecular weight tended to have higher use stage exposure. Chemicals with low intakes during use (<1%) and subsequent high post-use emissions, however, may yield comparable intake for a member of the general population. The presented PiF based framework offers a novel and critical advancement for life cycle assessments and high-throughput exposure screening of chemicals in cosmetic products demonstrating the importance of consistent consideration of near- and far-field multi pathway exposures. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Ernstoff, Alexi S.; Fantke, Peter] Tech Univ Denmark, Dept Engn Management, Quantitat Sustainabil Assessment Div, Prod Torvet 424, DK-2800 Lyngby, Denmark. [Ernstoff, Alexi S.; Csiszar, Susan A.; Henderson, Andrew D.; Chung, Susie; Jolliet, Olivier] Univ Michigan, Sch Publ Hlth, Environm Hlth Sci, Ann Arbor, MI 48109 USA. [Henderson, Andrew D.] US EPA, Sustainable Technol Div, Syst Anal Branch, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Csiszar, Susan A.] US EPA, Cincinnati, OH 45268 USA. RP Ernstoff, AS (reprint author), Tech Univ Denmark, Dept Engn Management, Quantitat Sustainabil Assessment Div, Prod Torvet 424, DK-2800 Lyngby, Denmark. EM alexer@dtu.dk RI Ernstoff, Alexi/P-4728-2016; OI Ernstoff, Alexi/0000-0002-1114-6596; Fantke, Peter/0000-0001-7148-6982 FU Long-Range Research Initiative of the American Chemistry Council [MTH1001-01]; Risk Science Center of the University of Michigan; Marie Curie project Tox-Train - European Commission under the Seventh Framework Programme [285286]; Dow Postdoctoral Fellowship FX The authors thank Dr. Tom McKone for input on mass transfer modeling, Dr. Charles Weschler for input on dermal exposure modeling, Dr. Annette Bunge for input on skin permeation data, Dr. Gerald Kasting and Matthew Miller for guidance, on dermal exposure modeling, and Dr. Alexis Laurent for internal review. This work was supported by the Long-Range Research Initiative of the American Chemistry Council (MTH1001-01), the Dow Postdoctoral Fellowship in Sustainability to S.A.C., the Risk Science Center of the University of Michigan and the Marie Curie project Tox-Train (grant agreement no. 285286) funded by the European Commission under the Seventh Framework Programme. Disclaimer: The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the US Environmental Protection Agency. NR 54 TC 3 Z9 3 U1 8 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD JUL-AUG PY 2016 VL 92-93 BP 87 EP 96 DI 10.1016/j.envint.2016.03.014 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ1HI UT WOS:000378951700010 PM 27062422 ER PT J AU Thayer, KA Schunemann, HJ AF Thayer, Kristina A. Schunemann, Holger J. TI Using GRADE to respond to health questions with different levels of urgency Preface SO ENVIRONMENT INTERNATIONAL LA English DT Editorial Material ID EVIDENCE-BASED MEDICINE; FETAL-GROWTH; GUIDELINES; QUALITY AB Increasing interest exists in applying the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach to environmental health evidence. While ideally applied to evidence synthesized in systematic reviews and corresponding summary tables, such as evidence profiles, GRADE's correct application requires that "the evidence that was assessed and the methods that were used to identify and appraise that evidence should be clearly described. In this article, we suggest that GRADE could be applied to evidence assembled from narrative reviews, modelled (indirect) evidence, or evidence assembled as part of a rapid response, if the underlying judgments about the certainty in this evidence are based on the relevant GRADE domains and provided transparently. Health questions that require assessing the certainty in a body of evidence to provide trustworthy answers may range from hours, to days or weeks, to a few months to scenarios that allow assessing evidence without short-term time pressures. Time frames of emergent, urgent or rapid evidence assessments will often require relying on existing summaries or rapidly compiling the available evidence and making assessments. Even without available full systematic reviews, expressing the certainty in the evidence can provide useful guidance for users of the evidence and those who evaluate certainty in effects. The ratings also help clarifying disagreement between organizations tackling similar questions about the evidence. Using the structured GRADE domains, narrative or other summaries of the evidence can be presented transparently. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Thayer, Kristina A.] Natl Inst Environm Hlth Sci, Div Natl Toxicol Program, NIH, Dept Hlth & Human Serv, POB 12233,Mail Drop K2-02, Res Triangle Pk, NC 27709 USA. [Schunemann, Holger J.] McMaster Univ, Dept Clin Epidemiol & Biostat, Dept Med, Hlth Sci Ctr, Room 2C14,1280 Main St West, Hamilton, ON L8S 4K1, Canada. RP Schunemann, HJ (reprint author), McMaster Univ, Dept Clin Epidemiol & Biostat, Dept Med, Hlth Sci Ctr, Room 2C14,1280 Main St West, Hamilton, ON L8S 4K1, Canada. EM thayer@niehs.nih.gov; schuneh@mcmaster.ca NR 28 TC 1 Z9 1 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD JUL-AUG PY 2016 VL 92-93 BP 585 EP 589 DI 10.1016/j.envint.2016.03.027 PG 5 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ1HI UT WOS:000378951700064 PM 27126781 ER PT J AU Cooper, GS Lunn, RM Agerstrand, M Glenn, BS Kraft, AD Luke, AM Ratcliffe, JM AF Cooper, Glinda S. Lunn, Ruth M. Agerstrand, Marlene Glenn, Barbara S. Kraft, Andrew D. Luke, April M. Ratcliffe, Jennifer M. TI Study sensitivity: Evaluating the ability to detect effects in systematic reviews of chemical exposures SO ENVIRONMENT INTERNATIONAL LA English DT Review DE Systematic review; Validity; Bias; Environmental health; Chemical hazard assessment; Study sensitivity ID ENVIRONMENTAL-HEALTH SCIENCE; SPONTANEOUS-ABORTIONS; COHORT; RISK; BIAS AB A critical step in systematic reviews of potential health hazards is the structured evaluation of the strengths and weaknesses of the included studies; risk of bias is a term often used to represent this process, specifically with respect to the evaluation of systematic errors that can lead to inaccurate (biased) results (i.e. focusing on internal validity). Systematic review methods developed in the clinical medicine arena have been adapted for use in evaluating environmental health hazards; this expansion raises questions about the scope of risk of bias tools and the extent to which they capture the elements that can affect the interpretation of results from environmental and occupational epidemiology studies and in vivo animal toxicology studies, (the studies typically available for assessment of risk of chemicals). One such element, described here as "sensitivity", is a measure of the ability of a study to detect a true effect or hazard. This concept is similar to the concept of the sensitivity of an assay; an insensitive study may fail to show a difference that truly exists, leading to a false conclusion of no effect Factors relating to study sensitivity should be evaluated in a systematic manner with the same rigor as the evaluation of other elements within a risk of bias framework. We discuss the importance of this component for the interpretation of individual studies, examine approaches proposed or in use to address it, and describe how it relates to other evaluation components. The evaluation domains contained within a risk of bias tool can include, or can be modified to include, some features relating to study sensitivity; the explicit inclusion of these sensitivity criteria with the same rigor and at the same stage of study evaluation as other bias-related criteria can improve the evaluation process. In some cases, these and other features may be better addressed through a separate sensitivity domain. The combined evaluation of risk of bias and sensitivity can be used to identify the most informative studies, to evaluate the confidence of the findings from individual studies and to identify those study elements that may help to explain heterogeneity across the body of literature. (C) 2016 Published by Elsevier Ltd. C1 [Cooper, Glinda S.; Glenn, Barbara S.; Kraft, Andrew D.; Luke, April M.] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Washington, DC 20460 USA. [Lunn, Ruth M.] NIEHS, Off Report Carcinogens, Div Natl Toxicol Program, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Agerstrand, Marlene] Stockholm Univ, Dept Environm Sci & Analyt Chem ACES, S-10691 Stockholm, Sweden. [Ratcliffe, Jennifer M.] ILS, Res Triangle Pk, NC USA. [Cooper, Glinda S.] One Potomac Yard South Bldg S-11342, Arlington, VA 22202 USA. RP Cooper, GS (reprint author), US EPA, 1200 Penn Ave NW,8601P, Washington, DC 20460 USA.; Cooper, GS (reprint author), One Potomac Yard South Bldg S-11342, Arlington, VA 22202 USA. EM cooper.glinda@epa.gov FU Intramural NIH HHS [Z99 ES999999] NR 24 TC 1 Z9 1 U1 4 U2 9 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD JUL-AUG PY 2016 VL 92-93 BP 605 EP 610 DI 10.1016/j.envint.2016.03.017 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ1HI UT WOS:000378951700067 PM 27156196 ER PT J AU Rooney, AA Cooper, GS Jahnke, GD Lam, J Morgan, RL Boyles, AL Ratcliffe, JM Kraft, AD Schunemann, HJ Schwingl, P Walker, TD Thayer, KA Lunn, RM AF Rooney, Andrew A. Cooper, Glinda S. Jahnke, Gloria D. Lam, Juleen Morgan, Rebecca L. Boyles, Abee L. Ratcliffe, Jennifer M. Kraft, Andrew D. Schunemann, Holger J. Schwingl, Pamela Walker, Teneille D. Thayer, Kristina A. Lunn, Ruth M. TI How credible are the study results? Evaluating and applying internal validity tools to literature-based assessments of environmental health hazards SO ENVIRONMENT INTERNATIONAL LA English DT Article DE Risk of bias; Internal validity; Systematic review; Environmental health; Hazard assessment ID INDUSTRY SPONSORSHIP; EVALUATION CRITERIA; RISK-ASSESSMENT; OF-INTEREST; QUALITY; METHODOLOGY; CONCLUSIONS; CAUSATION; EXPOSURE; SCIENCE AB Environmental health hazard assessments are routinely relied upon for public health decision-making. The evidence base used in these assessments is typically developed from a collection of diverse sources of information of varying quality. It is critical that literature-based evaluations consider the credibility of individual studies used to reach conclusions through consistent, transparent and accepted methods. Systematic review procedures address study credibility by assessing internal validity or "risk of bias" the assessment of whether the design and conduct of a study compromised the credibility of the link between exposure/intervention and outcome. This paper describes the commonalities and differences in risk-of-bias methods developed or used by five groups that conduct or provide methodological input for performing environmental health hazard assessments: the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) Working Group, the Navigation Guide, the National Toxicology Program's (NTP) Office of Health Assessment and Translation (OHAT) and Office of the Report on Carcinogens (ORoC), and the Integrated Risk Information System of the U.S. Environmental Protection Agency (EPA-IRIS). Each of these groups have been developing and applying rigorous assessment methods for integrating across a heterogeneous collection of human and animal studies to inform conclusions on potential-environmental health hazards. There is substantial consistency across the groups in the consideration of risk-of-bias issues or "domains' for assessing observational human studies. There is a similar overlap in terms of domains addressed for animal studies; however, the groups differ in the relative emphasis placed on-different aspects of risk of bias. Future directions for the continued harmonization and improvement of these methods are also discussed. Published by Elsevier Ltd. C1 [Rooney, Andrew A.; Boyles, Abee L.; Thayer, Kristina A.] NIEHS, Off Hlth Assessment & Translat, Div Natl Toxicol Program, NIH, Res Triangle Pk, NC 27560 USA. [Cooper, Glinda S.; Kraft, Andrew D.; Walker, Teneille D.] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Washington, DC 20460 USA. [Jahnke, Gloria D.; Lunn, Ruth M.] NIEHS, Off Report Carcinogens, Div Natl Toxicol Program, NIH, Res Triangle Pk, NC 27560 USA. [Lam, Juleen] Univ Calif San Francisco, Program Reprod Hlth & Environm, San Francisco, CA 94143 USA. [Morgan, Rebecca L.; Schunemann, Holger J.] McMaster Univ, Dept Clin Epidemiol & Biostat, Hamilton, ON, Canada. [Ratcliffe, Jennifer M.; Schwingl, Pamela] ILS, Morrisville, NC USA. RP Lunn, RM (reprint author), NIEHS, RTP, POB 12233,Mail Drop K2-04,NC 2770,530 Davis Dr, Morrisville, NC 27560 USA. EM lunn@niehs.nih.gov OI Boyles, Abee/0000-0002-8711-2077 FU Intramural NIH HHS [Z99 ES999999] NR 53 TC 3 Z9 3 U1 6 U2 9 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD JUL-AUG PY 2016 VL 92-93 BP 617 EP 629 DI 10.1016/j.envint.2016.01.005 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ1HI UT WOS:000378951700069 PM 26857180 ER PT J AU Samuel, GO Hoffmann, S Wright, RA Lalu, MM Patlewicz, G Becker, RA DeGeorge, GL Fergusson, D Hartung, T Lewis, RJ Stephens, ML AF Samuel, Gbeminiyi O. Hoffmann, Sebastian Wright, Robert A. Lalu, Manoj Mathew Patlewicz, Grace Becker, Richard A. DeGeorge, George L. Fergusson, Dean Hartung, Thomas Lewis, R. Jeffrey Stephens, Martin L. TI Guidance on assessing the methodological and reporting quality of toxicologically relevant studies: A scoping review SO ENVIRONMENT INTERNATIONAL LA English DT Review DE Methodological quality; Reporting quality; Risk of bias; Scoping review; Toxicity studies ID ENVIRONMENTAL RISK-ASSESSMENT; RANDOMIZED CONTROLLED-TRIALS; SYSTEMATIC REVIEWS; EVALUATION CRITERIA; BIAS; CHEMICALS; GUIDELINES; PROGRAM; HEALTH; REACH AB Assessments of methodological and reporting quality are critical to adequately judging the credibility of a study's conclusions and to gauging its potential reproducibility. To aid those seeking to assess the methodological or reporting quality of studies relevant to toxicology, we conducted a scoping review of the available guidance with respect to four types of studies: in vivo and in vitro, (quantitative) structure-activity relationships ([Q]SARs), physico-chernical, and human observational studies. Our aims were to identify the available guidance in this diverse literature, briefly summarize each document, and distill the common elements of these documents for each study type. In general, we found considerable guidance for in vivo and human studies, but only one paper addressed in vitro studies exclusively. The guidance for (Q)SAR studies and physico-chemical studies was scant but authoritative. There was substantial overlap across guidance documents in the proposed criteria for both methodological and reporting quality. Some guidance documents address toxicology research directly, whereas others address preclinical research generally or clinical research and therefore may not be fully applicable to the toxicology context without some translation. Another challenge is the degree to which assessments of methodological quality in toxicology should focus on risk of bias - as in clinical medicine and healthcare - or be broadened to include other quality measures, such as confirming the identity of test substances prior to exposure. Our review is intended primarily for those in toxicology and risk assessment seeking an entry point into the extensive and diverse literature on methodological and reporting quality applicable to their work. (C) 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Samuel, Gbeminiyi O.; Hartung, Thomas; Stephens, Martin L.] Johns Hopkins Ctr Alternat Anim Testing, 615 N Wolfe St, Baltimore, MD 21205 USA. [Hoffmann, Sebastian] Seh Consulting Serv, Stembergring 15, D-33106 Paderborn, Germany. [Wright, Robert A.] Johns Hopkins Univ, William H Welch Med Lib, 2024 E Monument St,Suite 1-200, Baltimore, MD 21287 USA. [Lalu, Manoj Mathew; Fergusson, Dean] Ottawa Hosp, Res Inst, Ottawa, ON K1Y 4E9, Canada. [Patlewicz, Grace] DuPont Haskell Global Ctr, 1090 Elkton Rd, Newark, DE 19711 USA. [Becker, Richard A.] Amer Chem Council, Div Sci & Res, 700 2nd St NE, Washington, DC 20002 USA. [DeGeorge, George L.] MB Res Labs, 1765 Wentz Rd, Spinnerstown, PA 18968 USA. [Lewis, R. Jeffrey] ExxonMobil Biomed Sci Inc, 1545 US Highway 22 East,Room LA 350, Annandale, NJ 08801 USA. [Patlewicz, Grace] US EPA, NCCT, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. RP Stephens, ML (reprint author), Johns Hopkins Ctr Alternat Anim Testing, 615 N Wolfe St, Baltimore, MD 21205 USA. EM gsamuel4@jhu.edu; Sebastian.hoffmann@seh-cs.com; rwrigh32@jhmi.edu; manojlalu@gmail.com; grace.y.tier@usa.dupont.com; rick_becker@americanchemistry.com; degeorge@mbresearch.com; dafergusson@ohri.ca; thartun1@jhu.edu; r.jeffrey.lewis@exxonmobil.com; msteph14@jhu.edu OI Hoffmann, Sebastian/0000-0002-3214-7678 FU ExxonMobil Foundation FX Authors GOS, SH, TH, and MLS wish to thank an anonymous philanthropic donor and the ExxonMobil Foundation for funding to the Johns Hopkins Center for Alternatives to Animal Testing (CAAT) to carry out this project of the Evidence-based Toxicology Collaboration (http://www.ebtox.com/), for which CAAT serves as secretariat. NR 75 TC 5 Z9 5 U1 5 U2 9 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD JUL-AUG PY 2016 VL 92-93 BP 630 EP 646 DI 10.1016/j.envint.2016.03.010 PG 17 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ1HI UT WOS:000378951700070 PM 27039952 ER PT J AU Joca, L Sacks, JD Moore, D Lee, JS Sams, R Cowden, J AF Joca, Lauren Sacks, Jason D. Moore, Danielle Lee, Janice S. Sams, Reeder, II Cowden, John TI Systematic review of differential inorganic arsenic exposure in minority, low-income, and indigenous populations in the United States SO ENVIRONMENT INTERNATIONAL LA English DT Review DE Arsenic; Environmental justice; Systematic review; Differential exposure ID ASSESSMENT SURVEY NHEXAS; WATER-SUPPLY-SYSTEM; EPA REGION V; DRINKING-WATER; SOCIOECONOMIC-STATUS; BLADDER-CANCER; WELL WATER; CARDIOVASCULAR-DISEASE; DIABETES-MELLITUS; NORTH-CAROLINA AB Inorganic arsenic (iAs) is a human carcinogen and associated with cardiovascular, respiratory, and skin diseases. Natural and anthropogenic sources contribute to low concentrations of iAs in water, food, soil, and air. Differential exposure to environmental hazards in minority, indigenous, and low income populations is considered an environmental justice (EJ) concern, yet it is unclear if higher iAs exposure occurs in these populations. A systematic review was conducted to evaluate evidence for differential iAs exposure in the United States (US). The peer reviewed literature was searched for studies that (1) estimated iAs exposure based on environmental concentrations of iAs in water, food, soil, or iAs biomarkers and (2) examined iAs exposure in minority, indigenous, and low income US populations. Five studies were identified that estimated exposures and provided demographic information about EJ populations. These studies reported arsenic concentrations in water, soil, or food to estimate exposure, with varied evidence of differential exposure. Additionally, six studies were identified that suggested potential arsenic exposure from environmental sources including soil, rice, private well-water, and fish, but did not report data stratified by demographic information. Evidence across these 11 studies was qualitatively integrated to draw conclusions about differential iAs exposure. The total body of evidence is limited by lack of individual exposure measures, lack of iAs concentration data, and insufficient comparative demographic data. Based upon these data gaps, there is inadequate evidence to conclude whether differential exposure to iAs is an EJ concern in the US. Published by Elsevier Ltd. C1 [Joca, Lauren; Moore, Danielle] US EPA, Hazardous Pollutant Assessment Grp, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. [Sacks, Jason D.] US EPA, Environm Media Assessment Grp, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. [Lee, Janice S.] US EPA, Integrated Risk Informat Syst Div, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. [Sams, Reeder, II; Cowden, John] US EPA, Hazardous Pollutant Assessment Grp, Natl Ctr Environm Assessment, Res Triangle Pk Div, Res Triangle Pk, NC 27711 USA. RP Cowden, J (reprint author), US EPA, Hazardous Pollutant Assessment Grp, Natl Ctr Environm Assessment, Res Triangle Pk Div, Res Triangle Pk, NC 27711 USA. EM cowden.john@epa.gov NR 66 TC 1 Z9 1 U1 13 U2 22 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD JUL-AUG PY 2016 VL 92-93 BP 707 EP 715 DI 10.1016/j.envint.2016.01.011 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ1HI UT WOS:000378951700075 PM 26896853 ER PT J AU Johnson, PI Koustas, E Vesterinen, HM Sutton, P Atchley, DS Kim, AN Campbell, M Donald, JM Sen, S Bero, L Zeise, L Woodruff, TJ AF Johnson, Paula I. Koustas, Erica Vesterinen, Hanna M. Sutton, Patrice Atchley, Dylan S. Kim, Allegra N. Campbell, Marlissa Donald, James M. Sen, Saunak Bero, Lisa Zeise, Lauren Woodruff, Tracey J. TI Application of the Navigation Guide systematic review methodology to the evidence for developmental and reproductive toxicity of triclosan SO ENVIRONMENT INTERNATIONAL LA English DT Review DE Soap; Personal care; Antibacterial; Thyroid; Policy; Risk assessment ID EVIDENCE-BASED MEDICINE; THYROID-FUNCTION; URINARY CONCENTRATIONS; ANTIBACTERIAL SOAPS; NEONATAL THYROXINE; EARLY-PREGNANCY; RISK-ASSESSMENT; HUMAN EXPOSURE; UNITED-STATES; CARE PRODUCTS AB Background: There are reports of developmental and reproductive health effects associated with the widely used biocide triclosan. Objective: Apply the Navigation Guide systematic review methodology to answer the question: Does exposure to triclosan have adverse effects on human development or reproduction? Methods: We applied the first 3 steps of the Navigation Guide methodology: 1) Specify a study question, 2) Select the evidence, and 3) Rate quality and strength of the evidence. We developed a protocol, conducted a comprehensive search of the literature, and identified relevant studies using pre-specified criteria. We assessed the number and type of all relevant studies. We evaluated each included study for risk of bias and rated the quality and strength of the evidence for the selected outcomes. We conducted a meta-analysis on a subset of suitable data. Results: We found 4282 potentially relevant records, and 81 records met our inclusion criteria. Of the more than 100 endpoints identified by our search, we focused our evaluation on hormone concentration outcomes, which had the largest human and non-human mammalian data set. Three human studies and 8 studies conducted in rats reported thyroxine levels as outcomes. The rat data were amenable to meta-analysis. Because only one of the human thyroxine studies quantified exposure, we did not conduct a meta-analysis of the human data. Through meta-analysis of the data for rats, we estimated for prenatal exposure a 0.09% (95% CI: 020, 0.02) reduction in thyroxine concentration per mg tridosan/kg-bw in fetal and young rats compared to control. For postnatal exposure we estimated a 031% (95% CI: -038, -023) reduction in thyroxine pet mg triclosan/kg-bw, also compared to control. Overall, we found low to moderate risk of bias across the human studies and moderate to high risk of bias across the non-human studies, and assigned a "moderate/low" quality rating to the body of evidence for human thyroid hormone alterations and a "moderate" quality rating to the body of evidence for non-human thyroid hormone alterations. Conclusion: Based on this application of the Navigation Guide systematic review methodology, we concluded that there was "sufficient' non-human evidence and "inadequate" human evidence of an association between triclosan exposure and thyroxine concentrations, and consequently, triclosan is "possibly toxic" to reproductive and developmental health. Thyroid hormone disruption is an upstream indicator of developmental toxicity. Additional endpoints may be identified as being of equal or greater concern as other data are developed or evaluated. (C) 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Johnson, Paula I.; Vesterinen, Hanna M.; Sutton, Patrice; Atchley, Dylan S.; Woodruff, Tracey J.] Univ Calif San Francisco, Program Reprod Hlth & Environm, Oakland, CA USA. [Koustas, Erica] US EPA, Off Policy, Natl Ctr Environm Econ, Washington, DC 20460 USA. [Kim, Allegra N.; Campbell, Marlissa; Donald, James M.; Zeise, Lauren] Calif Environm Protect Agcy, Off Environm Hlth Hazard Assessment, Sacramento, CA USA. [Sen, Saunak] Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA. [Bero, Lisa] Univ Calif San Francisco, Dept Clin Pharm, San Francisco, CA 94143 USA. [Bero, Lisa] Univ Sydney, Charles Perkins Ctr, Med Use & Hlth Outcomes, Sydney, NSW 2006, Australia. RP Johnson, PI (reprint author), Calif Dept Publ Hlth, Occupat Hlth Branch, Calif Safe Cosmet Program, 850 Marina Bay Pkwy, Richmond, CA 94804 USA. EM Paula.johnson@cdph.ca.gov FU CalEPA's Office of Environmental Health Hazard Assessment (OEHHA); U.S. EPA through Abt Associates [GAIA-0-6-UCSF 17288]; National Institute of Environmental Health Sciences [ESO22841]; U.S. EPA STAR grant [RD83543301]; Fred Gellert Foundation FX This research was funded by the CalEPA's Office of Environmental Health Hazard Assessment (OEHHA) and by the U.S. EPA through a contract with Abt Associates (GAIA-0-6-UCSF 17288), the National Institute of Environmental Health Sciences (ESO22841), U.S. EPA STAR grant (RD83543301), and the Fred Gellert Foundation. The research was supported in part by appointments to the Internship/Research Participation Program at the National Center for Environmental Economics, U.S. EPA, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and U.S. EPA. NR 84 TC 4 Z9 4 U1 4 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD JUL-AUG PY 2016 VL 92-93 BP 716 EP 728 DI 10.1016/j.envint.2016.03.009 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ1HI UT WOS:000378951700076 PM 27156197 ER PT J AU Augustine, SAJ AF Augustine, Swinburne A. J. TI Towards Universal Screening for Toxoplasmosis: Rapid, Cost-Effective, and Simultaneous Detection of Anti-Toxoplasma IgG, IgM, and IgA Antibodies by Use of Very Small Serum Volumes SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID CONGENITAL TOXOPLASMOSIS; IMMUNOASSAY; RESPONSES; GONDII; PATHOGENS; INFANTS AB Rapid, cost-effective, and early determination of the serological status of potentially infected individuals, particularly pregnant women, can be critical in preventing life-threatening infections and subsequent fetal congenital abnormalities. An article in this issue of the Journal of Clinical Microbiology ( X. Li, C. Pomares, G. Gonfrier, B. Koh, S. Zhu, M. Gong, J. G. Montoya, and H. Dai, J Clin Microbiol 54: 1726-1733, 2016, http://dx.doi.org/10.1128/JCM.03371-15) describes an innovative multiplexed immunoassay that offers a path toward universal screening. C1 [Augustine, Swinburne A. J.] US EPA, Natl Exposure Res Lab, Exposure Methods & Measurement Div, Microbial Exposure Branch, Cincinnati, OH 45268 USA. RP Augustine, SAJ (reprint author), US EPA, Natl Exposure Res Lab, Exposure Methods & Measurement Div, Microbial Exposure Branch, Cincinnati, OH 45268 USA. EM Augustine.swinburne@ega.gov FU USEPA; Office of Research and Development, U.S. Environmental Protection Agency FX This work, including the efforts of Swinburne A. J. Augustine, was funded by USEPA.; The author's research is funded by the Office of Research and Development, U.S. Environmental Protection Agency. NR 26 TC 1 Z9 1 U1 3 U2 6 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD JUL PY 2016 VL 54 IS 7 BP 1684 EP 1685 DI 10.1128/JCM.03371-15 PG 2 WC Microbiology SC Microbiology GA DP6WZ UT WOS:000378641600005 PM 27170019 ER PT J AU Kraft, AD McPherson, CA Harry, GJ AF Kraft, Andrew D. McPherson, Christopher A. Harry, G. Jean TI Association Between Microglia, Inflammatory Factors, and Complement with Loss of Hippocampal Mossy Fiber Synapses Induced by Trimethyltin SO NEUROTOXICITY RESEARCH LA English DT Article DE Tumor necrosis factor; C1q; M1/M2 polarization; Interleukin 1; Synapse stripping; Microglia ID CENTRAL-NERVOUS-SYSTEM; APOPTOTIC CELLS; IN-VIVO; SYNAPTIC TERMINALS; ALZHEIMERS-DISEASE; CEREBRAL-ISCHEMIA; GRANULE NEURONS; BRAIN MICROGLIA; MESSENGER-RNA; RAT-BRAIN AB Complement-associated factors are implicated in pathogen presentation, neurodegeneration, and microglia resolution of tissue injury. To characterize complement activation with microglial clearance of degenerating mossy fiber boutons, hippocampal dentate granule neurons were ablated in CD-1 mice with trimethyltin (TMT; 2.2 mg/kg, i.p.). Neuronal apoptosis was accompanied by amoeboid microglia and elevations in tumor necrosis factor [Tnfa], interleukin 1 beta [Il1b], and Il6 mRNA and C1q protein. Inos mRNA levels were unaltered. Silver degeneration and synaptophysin staining indicated loss of synaptic innervation to CA3 pyramidal neurons. Reactive microglia with thickened bushy morphology showed co-localization of synaptophysin+ fragments. The initial response at 2 days post-TMT included transient elevations in Tnfa, Il1b, Il6, and Inos mRNA levels. A concurrent increase at 2 days was observed in arginase-1 [Arg1], Il10, transforming growth factor beta 1 [Tgfb1], and chitinase 3 like-3 [Ym1] mRNA levels. At 2 days, C1q protein was evident in the CA3 with elevated C1qa, C1qb, C3, Cr3a, and Cr3b mRNA levels. mRNA levels remained elevated at 5 days, returning to control by 14 days, corresponding to silver degeneration. mRNA levels for pentraxin3 (Ptx3) were elevated on day 2 and Ptx1 was not altered. Our data suggest an association between microglia reactivity, the induction of anti-inflammatory genes concurrent with pro-inflammatory genes and the expression of complement-associated factors with the degeneration of synapses following apoptotic neuronal loss. C1 [Kraft, Andrew D.; McPherson, Christopher A.; Harry, G. Jean] NIEHS, Neurotoxicol Grp, Natl Toxicol Program Lab, NIH, POB 12233,Mail Drop E1-07, Res Triangle Pk, NC 27709 USA. [Kraft, Andrew D.] US EPA, Natl Ctr Environm Assessment, Washington, DC 20460 USA. RP Harry, GJ (reprint author), NIEHS, Neurotoxicol Grp, Natl Toxicol Program Lab, NIH, POB 12233,Mail Drop E1-07, Res Triangle Pk, NC 27709 USA. EM kraft.andrew@epa.gov; harry@niehs.nih.gov FU Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services [1Z01ES101623, ES021164]; Division of National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services [1Z01ES101623, ES021164] FX This research was supported by the Division of Intramural Research and the Division of National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services #1Z01ES101623 and ES021164. The authors graciously acknowledge the generous gift of C1q antibody from Dr. Andrea Tenner at the University of California-Irvine, and FD NeuroTechnologies for the CuAg staining. The views expressed in this article are those of the authors and they do not necessarily represent the views or policies of the US EPA. NR 87 TC 0 Z9 0 U1 2 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1029-8428 EI 1476-3524 J9 NEUROTOX RES JI Neurotox. Res. PD JUL PY 2016 VL 30 IS 1 SI SI BP 53 EP 66 DI 10.1007/s12640-016-9606-8 PG 14 WC Neurosciences SC Neurosciences & Neurology GA DQ2UG UT WOS:000379058100005 PM 26892644 ER PT J AU Dionisio, KL Baxter, LK Burke, J Ozkaynak, H AF Dionisio, Kathie L. Baxter, Lisa K. Burke, Janet Ozkaynak, Haluk TI The importance of the exposure metric in air pollution epidemiology studies: When does it matter, and why? SO AIR QUALITY ATMOSPHERE AND HEALTH LA English DT Article DE Air quality models; Exposure error; Exposure metric; Exposure models; Uncertainty; Epidemiology; Air pollution ID AMBIENT OZONE CONCENTRATIONS; FINE PARTICULATE MATTER; TIME-SERIES; MEASUREMENT ERROR; PM2.5 EXPOSURE; SOUTHERN CALIFORNIA; CHILDHOOD ASTHMA; NITROGEN-DIOXIDE; BIRTH OUTCOMES; HEALTH AB Exposure error in ambient air pollution epidemiologic studies may introduce bias and/or attenuation of the health risk estimate, reduce statistical significance, and lower statistical power. Alternative exposure metrics are increasingly being used in place of central-site measurements, with the intent of reducing exposure error. Dependent on the study design, health outcome, and pollutant of interest, these metrics may provide a means of reducing error (leading to less bias and uncertainty in health risk estimates) if they capture variability in exposure which is not represented when central-site measurements are used. We examine the current evidence for answering the question of when the choice of exposure metric matters and why, focusing on studies which examined multiple exposure metrics in the same epidemiologic study. We conclude that for time-series and case-crossover studies, central-site measurements may be sufficient, especially for homogeneous pollutants, and in cohort and panel studies, approaches that increase spatial resolution of the exposure metrics do impact the health effect estimates. We note that while the current literature is widely dispersed across exposure metrics and health outcomes, the largest collective, common body of literature is focused on birth/pregnancy outcomes and traffic-related pollution. Also additional discussion and agreement is needed on how to classify metrics as "different" and "better." Primary recommendations are to provide context for the reasons behind selection of exposure metrics and to encourage collaboration between exposure scientists and epidemiologists when designing and implementing a study, as results can have important implications for the development of policies and regulations. C1 [Dionisio, Kathie L.; Baxter, Lisa K.; Burke, Janet; Ozkaynak, Haluk] US EPA, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. RP Dionisio, KL (reprint author), US EPA, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM kathie.dionisio@gmail.com NR 48 TC 1 Z9 1 U1 10 U2 10 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 1873-9318 EI 1873-9326 J9 AIR QUAL ATMOS HLTH JI Air Qual. Atmos. Health PD JUL PY 2016 VL 9 IS 5 BP 495 EP 502 DI 10.1007/s11869-015-0356-1 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP6AP UT WOS:000378579600005 ER PT J AU Hines, RN Simpson, PM McCarver, DG AF Hines, Ronald N. Simpson, Pippa M. McCarver, D. Gail TI Age-Dependent Human Hepatic Carboxylesterase 1 (CES1) and Carboxylesterase 2 (CES2) Postnatal Ontogeny SO DRUG METABOLISM AND DISPOSITION LA English DT Article ID IN-VITRO METABOLISM; DEVELOPMENTAL EXPRESSION; HUMAN LIVER; HYDROLYTIC METABOLISM; DRUG-METABOLISM; OSELTAMIVIR; IRINOTECAN; RESPONSIVENESS; IDENTIFICATION; DELTAMETHRIN AB Human hepatic carboxylesterase 1 and 2 (CES1 and CES2) are important for the disposition of ester-and amide-bond-containing pharmaceuticals and environmental chemicals. CES1 and CES2 ontogeny has not been well characterized, causing difficulty in addressing concerns regarding juvenile sensitivity to adverse outcomes associated with exposure to certain substrates. To characterize postnatal human hepatic CES1 and CES2 expression, microsomal and cytosolic fractions were prepared using liver samples from subjects without liver disease (N = 165, aged 1 day to 18 years). Proteins were fractionated, detected, and quantitated by Western blotting. Median microsomal CES1 was lower among samples from subjects younger than 3 weeks (n = 36) compared with the rest of the population (n = 126; 6.27 vs. 17.5 pmol/mg microsomal protein, respectively; P < 0.001; Kruskal-Wallis test). Median cytosolic CES1 expression was lowest among samples from individuals between birth and 3 weeks of age (n = 36), markedly greater among those aged 3 weeks to 6 years (n = 90), and modestly greater still among those older than 6 years (n = 36; median values = 4.7, 15.8, and 16.6 pmol/mg cytosolic protein, respectively; P values < 0.001 and 0.05, respectively; Kruskal-Wallis test). Median microsomal CES2 expression increased across the same three age groups with median values of 1.8, 2.9, and 4.2 pmol/mg microsomal protein, respectively (P < 0.001, both). For cytosolic CES2, only the youngest age group differed from the two older groups (P < 0.001; median values = 1.29, 1.93, 2.0, respectively). These data suggest that infants younger than 3 weeks of age would exhibit significantly lower CES1- and CES2-dependent metabolic clearance compared with older individuals. C1 [Hines, Ronald N.; Simpson, Pippa M.; McCarver, D. Gail] Med Coll Wisconsin, Dept Pediat, 8701 Watertown Plank Rd, Milwaukee, WI 53226 USA. [Hines, Ronald N.; McCarver, D. Gail] Med Coll Wisconsin, Dept Pharmacol Toxicol, Milwaukee, WI 53226 USA. Childrens Hosp & Hlth Syst, Childrens Res Inst, Milwaukee, WI USA. [Hines, Ronald N.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. RP Hines, RN (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM hines.ronald@epa.gov FU Council for the Advancement of Pyrethroid Human Risk Assessment FX This study was funded by the Council for the Advancement of Pyrethroid Human Risk Assessment. NR 27 TC 3 Z9 3 U1 3 U2 5 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 0090-9556 EI 1521-009X J9 DRUG METAB DISPOS JI Drug Metab. Dispos. PD JUL PY 2016 VL 44 IS 7 BP 959 EP 966 DI 10.1124/dmd.115.068957 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA DP7TJ UT WOS:000378701800010 PM 26825642 ER PT J AU Thomson, MMS Hines, RN Schuetz, EG Meibohm, B AF Thomson, Margaret M. S. Hines, Ronald N. Schuetz, Erin G. Meibohm, Bernd TI Expression Patterns of Organic Anion Transporting Polypeptides 1B1 and 1B3 Protein in Human Pediatric Liver SO DRUG METABOLISM AND DISPOSITION LA English DT Article ID BILE-ACID; DEVELOPMENTAL EXPRESSION; DRUG DEVELOPMENT; PHASE-I; ONTOGENY; INDUCTION; CHILDREN; RECEPTOR; INFANTS; SLC21A8 AB Determining appropriate pharmacotherapy in young children can be challenging due to uncertainties in the development of drug disposition pathways. With knowledge of the ontogeny of drug-metabolizing enzymes and an emerging focus on drug transporters, the developmental pattern of the uptake transporters organic anion transporting polypeptide (OATP) 1B1 and 1B3 was assessed by relative protein quantification using Western blotting in 80 human pediatric liver specimens covering an age range from 9 days to 12 years. OATP1B3 exhibited high expression at birth, which declined over the first months of life, and then increased again in the preadolescent period. In comparison with children 6-12 years of age, the relative protein expression of highly glycosylated (total) OATP1B3 was 235% (357%) in children < 3 months of age, 33% (64%) in the age group from 3 months to 2 years, and 50% (59%) in children 2-6 years of age. The fraction of highly glycosylated to total OATP1B3 increased with age, indicating ontogenic processes not only at the transcriptional level but also at the post-translational level. Similar to OATP1B3, OATP1B1 showed high interindividual variability in relative protein expression but no statistically significant difference among the studied age groups. C1 [Thomson, Margaret M. S.; Meibohm, Bernd] Univ Tennessee, Hlth Sci Ctr, Dept Pharmaceut Sci, Memphis, TN 38163 USA. [Hines, Ronald N.] Med Coll Wisconsin, Dept Pediat, 8701 Watertown Plank Rd, Milwaukee, WI 53226 USA. [Schuetz, Erin G.] St Jude Childrens Res Hosp Memphis, Dept Pharmaceut Sci, Memphis, TN USA. [Hines, Ronald N.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. RP Meibohm, B (reprint author), Univ Tennessee, Hlth Sci Ctr, Coll Pharm, 881 Madison Ave,Room 444, Memphis, TN 38163 USA. EM bmeibohm@uthsc.edu OI Thomson, Margaret/0000-0002-2147-6943 FU National Institutes of Health National Cancer Institute [R01-CA-53106]; American Lebanese Syrian Associated Charities (ALSAC) FX This work was in part supported by the National Institutes of Health National Cancer Institute [Grant R01-CA-53106]; and the American Lebanese Syrian Associated Charities (ALSAC). NR 36 TC 1 Z9 1 U1 2 U2 4 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 0090-9556 EI 1521-009X J9 DRUG METAB DISPOS JI Drug Metab. Dispos. PD JUL PY 2016 VL 44 IS 7 BP 999 EP 1004 DI 10.1124/dmd.115.069252 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA DP7TJ UT WOS:000378701800015 PM 27098745 ER PT J AU Giebel, NL Shadley, JD McCarver, DG Dorko, K Gramignoli, R Strom, SC Yan, K Simpson, PM Hines, RN AF Giebel, Nicholas L. Shadley, Jeffrey D. McCarver, D. Gail Dorko, Kenneth Gramignoli, Roberto Strom, Stephen C. Yan, Ke Simpson, Pippa M. Hines, Ronald N. TI Role of Chromatin Structural Changes in Regulating Human CYP3A Ontogeny SO DRUG METABOLISM AND DISPOSITION LA English DT Article ID ENRICHED TRANSCRIPTION FACTORS; HISTONE MODIFICATIONS; STEM-CELLS; DEVELOPMENTAL EXPRESSION; LIVER MATURATION; DRUG-METABOLISM; HUMAN GENOME; MOUSE-LIVER; CYTOCHROME-P450; PATTERNS AB Variability in drug-metabolizing enzyme developmental trajectories contributes to interindividual differences in susceptibility to chemical toxicity and adverse drug reactions, particularly in the first years of life. Factors linked to these interindividual differences are largely unknown, but molecular mechanisms regulating ontogeny are likely involved. To evaluate chromatin structure dynamics as a likely contributing mechanism, age-dependent changes in modified and variant histone occupancy were evaluated within known CYP3A4 and 3A7 regulatory domains. Chromatin immunoprecipitation using fetal or postnatal human hepatocyte chromatin pools followed by quantitative polymerase chain reaction DNA amplification was used to determine relative chromatin occupancy by modified and variant histones. Chromatin structure representing a poised transcriptional state (bivalent chromatin), indicated by the occupancy by modified histones associated with both active and repressed transcription, was observed for CYP3A4 and most 3A7 regulatory regions in both postnatal and fetal livers. However, the CYP3A4 regulatory regions had significantly greater occupancy by modified histones associated with repressed transcription in the fetal liver. Conversely, some modified histones associated with active transcription exhibited greater occupancy in the postnatal liver. CYP3A7 regulatory regions also had significantly greater occupancy by modified histones associated with repressed transcription in the fetus. The observed occupancy by modified histones is consistent with chromatin structural dynamics contributing to CYP3A4 ontogeny, although the data are less conclusive regarding CYP3A7. Interpretation of the latter data may be confounded by celltype heterogeneity in the fetal liver. C1 [Giebel, Nicholas L.; Shadley, Jeffrey D.; McCarver, D. Gail; Yan, Ke; Simpson, Pippa M.; Hines, Ronald N.] Med Coll Wisconsin, Dept Pediat, 8701 Watertown Plank Rd, Milwaukee, WI 53226 USA. [Giebel, Nicholas L.; Shadley, Jeffrey D.; McCarver, D. Gail; Yan, Ke; Simpson, Pippa M.; Hines, Ronald N.] Med Coll Wisconsin, Dept Pharmacol & Toxicol, Milwaukee, WI 53226 USA. [Giebel, Nicholas L.; Shadley, Jeffrey D.; McCarver, D. Gail; Yan, Ke; Simpson, Pippa M.; Hines, Ronald N.] Childrens Hosp & Hlth Syst, Childrens Res Inst, Milwaukee, WI USA. [Dorko, Kenneth; Gramignoli, Roberto; Strom, Stephen C.] Univ Pittsburgh, Dept Pathol, Pittsburgh, PA USA. [Shadley, Jeffrey D.] Univ Michigan Hlth Syst, Dept Pediat Crit Care, Ann Arbor, MI USA. [Dorko, Kenneth] Samsara Sci, San Diego, CA USA. [Gramignoli, Roberto; Strom, Stephen C.] Karolinska Hosp & Inst, Dept Lab Med, Stockholm, Sweden. [Hines, Ronald N.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr,MD 305-02, Res Triangle Pk, NC 27711 USA. RP Hines, RN (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr,MD 305-02, Res Triangle Pk, NC 27711 USA. EM hines.ronald@epa.gov FU National Institutes of Health [GM081344]; Swedish Research Council; Torsten och Ragnar Soderberg Stiftelse; Ventenskapradet FX This work was supported in part by the National Institutes of Health [Grant GM081344] (R.N.H. and D.G.M.) and the Swedish Research Council, Ventenskapradet and the Torsten och Ragnar Soderberg Stiftelse (R.G. and S.C.S.). NR 49 TC 2 Z9 2 U1 2 U2 5 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 0090-9556 EI 1521-009X J9 DRUG METAB DISPOS JI Drug Metab. Dispos. PD JUL PY 2016 VL 44 IS 7 BP 1027 EP 1037 DI 10.1124/dmd.116.069344 PG 11 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA DP7TJ UT WOS:000378701800019 PM 26921389 ER PT J AU Liu, XY Guo, ZS Krebs, KA Greenwell, DJ Roache, NF Stinson, RA Nardin, JA Pope, RH AF Liu, Xiaoyu Guo, Zhishi Krebs, Kenneth A. Greenwell, Dale J. Roache, Nancy F. Stinson, Rayford A. Nardin, Joshua A. Pope, Robert H. TI Laboratory study of PCB transport from primary sources to building materials SO INDOOR AND BUILT ENVIRONMENT LA English DT Article DE Polychlorinated biphenyls; Sink effect; Chamber testing; Building materials; Material; air partition coefficient; Diffusion coefficient ID SEMIVOLATILE ORGANIC-COMPOUNDS; STEADY-STATE CONCENTRATIONS; POLYCHLORINATED-BIPHENYLS; PARTITION-COEFFICIENTS; DIFFUSION-COEFFICIENTS; COMPOUNDS INDOORS; VOLATILE; EMISSIONS; SORPTION; FRAMEWORK AB The sorption of airborne polychlorinated biphenyls (PCBs) by 20 building materials and their re-emission (desorption) from concrete were investigated using two 0.053 m(3) environmental chambers connected in series with a field-collected caulk in the source chamber serving as a stable source of PCBs and building materials in the test chamber. During the tests, the PCB concentrations in the outlet air of the test chamber were monitored and the building materials were removed from the test chamber at different times to determine their PCB content. Among the materials tested, a petroleum-based paint, a latex paint, and a certain type of carpet were among the strongest sinks. Solvent-free epoxy coating, certain types of flooring materials, and brick were among the weakest sinks. For a given sink material, PCB congeners with lower vapour pressures were sorbed in larger quantities. Rough estimates of the partition and diffusion coefficients were obtained by applying a sink model to the data acquired from the chamber studies. A desorption test with the concrete panels showed that re-emission is a slow process, suggesting that PCB sinks, e.g. concrete, can release PCBs into the air for a prolonged period of time (years or decades). This study could fill some of the data gaps associated with the characterization of PCB sinks in contaminated buildings. C1 [Liu, Xiaoyu; Guo, Zhishi; Krebs, Kenneth A.; Greenwell, Dale J.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Roache, Nancy F.; Stinson, Rayford A.; Nardin, Joshua A.; Pope, Robert H.] US Inc, Arcadis, Durham, NC USA. RP Liu, XY (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. EM liu.xiaoyu@epa.gov NR 42 TC 2 Z9 2 U1 6 U2 9 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1420-326X EI 1423-0070 J9 INDOOR BUILT ENVIRON JI Indoor Built Environ. PD JUL PY 2016 VL 25 IS 4 BP 635 EP 650 DI 10.1177/1420326X15623355 PG 16 WC Construction & Building Technology; Engineering, Environmental; Public, Environmental & Occupational Health SC Construction & Building Technology; Engineering; Public, Environmental & Occupational Health GA DP3WS UT WOS:000378427200006 ER PT J AU Ahir, BK Pratten, MK AF Ahir, Bhavesh K. Pratten, Margaret K. TI The impact of caffeine on connexin expression in the embryonic chick cardiomyocyte micromass culture system SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE Caffeine; Congenital heart defects; Embryonic chick cardiomyocytes micromass (MM) culture; Embryotoxicity/Teratogenicity; Gap junction proteins (Cx40, Cx43, Cx45) ID VITRO EMBRYOTOXICITY TESTS; IN-VITRO; GAP-JUNCTIONS; PREDICTION MODELS; CARDIOVASCULAR-SYSTEM; CARDIAC MYOCYTES; NERVOUS-SYSTEM; TERATOGEN TEST; HEART; TOXICITY AB Cardiomyocytes are electrically coupled by gap junctions, defined as clusters of low-resistance multisubunit transmembrane channels composed of connexins (Cxs). The expression of Cx40, Cx43 and Cx45, which are present in cardiomyocytes, is known to be developmentally regulated. This study investigates the premise that alterations in gap junction proteins are one of the mechanisms by which teratogens may act. Specifically, those molecules known to be teratogenic in humans could cause their effects via disruption of cell-to-cell communication pathways, resulting in an inability to co-ordinate tissue development. Caffeine significantly inhibited contractile activity at concentrations above and including 1500 mu M (P < 0.05), while not affecting cell viability and total protein, in the embryonic chick cardiomyocyte micromass culture system. The effects of caffeine on key cardiac gap junction protein (Cx40, Cx43 and Cx45) expression were analysed using immunocytochemistry and in-cell Western blotting. The results indicated that caffeine altered the expression pattern of Cx40, Cx43 and Cx45 at non-cytotoxic concentrations (>= 2000 mu M), i.e., at concentrations that did not affect total cell protein and cell viability. In addition the effects of caffeine on cardiomyocyte formation and function (contractile activity score) were correlated with modulation of Cxs (Cx40, Cx43 and Cx45) expression, at above and including 2000 mu M caffeine concentrations (P < 0.05). These experiments provide evidence that embryonic chick cardiomyocyte micromass culture may be a useful in vitro method for mechanistic studies of perturbation of embryonic heart development. Copyright (C) 2015 John Wiley & Sons, Ltd. C1 [Ahir, Bhavesh K.] US EPA, Natl Ctr Computat Toxicol B205 01, Res Triangle Pk, NC 27711 USA. [Pratten, Margaret K.] Univ Nottingham, Sch Life Sci, Queens Med Ctr, Nottingham NG7 2UH, England. RP Pratten, MK (reprint author), Univ Nottingham, Sch Life Sci, Queens Med Ctr, Nottingham NG7 2UH, England. EM margaret.pratten@nottingham.ac.uk FU Developing Research Scholarship, the International Office of the University of Nottingham, UK FX This work was funded by Developing Research Scholarship, the International Office of the University of Nottingham, UK. NR 71 TC 0 Z9 0 U1 3 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD JUL PY 2016 VL 36 IS 7 BP 903 EP 913 DI 10.1002/jat.3219 PG 11 WC Toxicology SC Toxicology GA DP5XM UT WOS:000378571500004 PM 26304238 ER PT J AU Lowry, JA Ahdoot, S Baum, CR Bernstein, AS Bole, A Brumberg, HL Campbell, CC Lanphear, BP Pacheco, SE Spanier, AJ Trasande, L Osterhoudt, KC Paulson, JA Sandel, MT Rogers, PT Balbus, JM Brubaker, TA DeNicola, NG Etzel, RA Mortensen, ME Ward, MH Spire, P AF Lowry, Jennifer A. Ahdoot, Samantha Baum, Carl R. Bernstein, Aaron S. Bole, Aparna Brumberg, Heather Lynn Campbell, Carla C. Lanphear, Bruce Perrin Pacheco, Susan E. Spanier, Adam J. Trasande, Leonardo Osterhoudt, Kevin C. Paulson, Jerome A. Sandel, Megan T. Rogers, Paul Thomas Balbus, John M. Brubaker, Todd A. DeNicola, Nathaniel G. Etzel, Ruth Ann Mortensen, Mary Ellen Ward, Mary H. Spire, Paul TI Prevention of Childhood Lead Toxicity SO PEDIATRICS LA English DT Article ID ELEVATED BLOOD LEAD; US CHILDREN; EARLY ADULTHOOD; INFANT FORMULA; UNITED-STATES; EXPOSURE; LEVEL; WATER; DUST; RISK AB Blood lead concentrations have decreased dramatically in US children over the past 4 decades, but too many children still live in housing with deteriorated lead-based paint and are at risk for lead exposure with resulting lead-associated cognitive impairment and behavioral problems. Evidence continues to accrue that commonly encountered blood lead concentrations, even those below 5 mu g/dL (50 ppb), impair cognition; there is no identified threshold or safe level of lead in blood. From 2007 to 2010, approximately 2.6% of preschool children in the United States had a blood lead concentration >= 5 mu g/dL (>= 50 ppb), which represents about 535 000 US children 1 to 5 years of age. Evidence-based guidance is available for managing increased lead exposure in children, and reducing sources of lead in the environment, including lead in housing, soil, water, and consumer products, has been shown to be cost-beneficial. Primary prevention should be the focus of policy on childhood lead toxicity. C1 [DeNicola, Nathaniel G.] Amer Coll Obstetricians & Gynecologists, Washington, DC 20024 USA. [Etzel, Ruth Ann] US Environm Protect Agcy, Washington, DC 20004 USA. [Mortensen, Mary Ellen] CDC, Natl Ctr Environm Hlth, Atlanta, GA 30333 USA. [Ward, Mary H.] NCI, Bethesda, MD 20892 USA. NR 71 TC 0 Z9 0 U1 11 U2 15 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 EI 1098-4275 J9 PEDIATRICS JI Pediatrics PD JUL PY 2016 VL 138 IS 1 AR e20161493 DI 10.1542/peds.2016-1493 PG 15 WC Pediatrics SC Pediatrics GA DP9YD UT WOS:000378853100071 ER PT J AU Burkhard, LP Hubin-Barrows, D Billa, N Highland, TL Hockett, JR Mount, DR Norberg-King, TJ AF Burkhard, Lawrence P. Hubin-Barrows, Dylan Billa, Nanditha Highland, Terry L. Hockett, James R. Mount, David R. Norberg-King, Teresa J. TI Sediment Bioaccumulation Test with Lumbriculus variegatus: Effects of Organism Loading SO ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY LA English DT Article ID TOXICITY; WATER; CONTAMINANTS; OLIGOCHAETE; RESIDUES; FIELD AB At contaminated sediment sites, the bioavailability of contaminants in sediments is assessed using sediment-bioaccumulation tests with Lumbriculus variegates (Lv). The testing protocols recommend that ratio of total organic carbon (TOC) in sediment to L. variegatus (dry weight) (TOC/Lv) should be no less than 50:1. Occasionally, this recommendation is not followed, especially with sediments having low TOC, e.g., < 1 %. This study evaluated the impacts and resulting biases in the testing results when the recommendation of "no less than 50:1" is not followed. In the study, seven sediments were tested with a series of TOC/Lv ratios that spanned the recommendation. With increasing loading of organisms, growth of the organisms decreased in six of the seven sediments tested. Residues of polychlorinated biphenyls (PCBs) in the L. variegatus were measured in six of the seven sediments tested, and differences in PCB residues among loading ratios across all sediments were small, i.e., +/- 50 %, from those measured at the minimum recommended ratio of 50:1 TOC/Lv. In all sediment, PCB residues increased with increasing loading of the organisms for the mono-, di-, and tri-chloro-PCBs. For tetra-chloro and heavier PCBs, residues increased with increasing loading of organisms for only two of the six sediments. PCB residues were not significantly different between TOC/Lv loadings of 50:1 and mid-20:1 ratios indicating that equivalent results can be obtained with TOC/Lv ratios into the mid-20:1 ratios. Overall, the testing results suggest that when testing recommendation of 50:1 TOC/Lv is not followed, potential biases in the biota-sediment accumulations factors from the sediment-bioaccumulation test will be small. C1 [Burkhard, Lawrence P.; Highland, Terry L.; Hockett, James R.; Mount, David R.; Norberg-King, Teresa J.] US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Duluth, MN 55804 USA. [Hubin-Barrows, Dylan] 3 M Co, Cumberland, WI 54829 USA. [Billa, Nanditha] Wildlife Int, Easton, MD 21601 USA. RP Burkhard, LP (reprint author), US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Duluth, MN 55804 USA. EM burkhard.lawrence@epa.gov NR 25 TC 0 Z9 0 U1 3 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0090-4341 EI 1432-0703 J9 ARCH ENVIRON CON TOX JI Arch. Environ. Contam. Toxicol. PD JUL PY 2016 VL 71 IS 1 BP 70 EP 77 DI 10.1007/s00244-016-0284-6 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DO7ZD UT WOS:000378000900008 PM 27165691 ER PT J AU Clippinger, AJ Ahluwalia, A Allen, D Bonner, JC Casey, W Castranova, V David, RM Halappanavar, S Hotchkiss, JA Jarabek, AM Maier, M Polk, W Rothen-Rutishauser, B Sayes, CM Sayre, P Sharma, M Stone, V AF Clippinger, Amy J. Ahluwalia, Arti Allen, David Bonner, James C. Casey, Warren Castranova, Vincent David, Raymond M. Halappanavar, Sabina Hotchkiss, Jon A. Jarabek, Annie M. Maier, Monika Polk, William Rothen-Rutishauser, Barbara Sayes, Christie M. Sayre, Phil Sharma, Monita Stone, Vicki TI Expert consensus on an in vitro approach to assess pulmonary fibrogenic potential of aerosolized nanomaterials SO ARCHIVES OF TOXICOLOGY LA English DT Article DE Inhalation toxicity; Multi-walled carbon nanotubes; MWCNTs; In vitro testing strategies; Regulatory risk assessment; Pulmonary fibrosis ID MULTIWALL CARBON NANOTUBES; AIRWAY EPITHELIAL-CELLS; HUMAN RESPIRATORY-TRACT; LINING FLUID ANTIOXIDANTS; SILVER-NANOPARTICLES; LUNG-CELLS; ENGINEERED NANOMATERIALS; INHALATION TOXICITY; PARTICLE DEPOSITION; LIQUID INTERFACE AB The increasing use of multi-walled carbon nanotubes (MWCNTs) in consumer products and their potential to induce adverse lung effects following inhalation has lead to much interest in better understanding the hazard associated with these nanomaterials (NMs). While the current regulatory requirement for substances of concern, such as MWCNTs, in many jurisdictions is a 90-day rodent inhalation test, the monetary, ethical, and scientific concerns associated with this test led an international expert group to convene in Washington, DC, USA, to discuss alternative approaches to evaluate the inhalation toxicity of MWCNTs. Pulmonary fibrosis was identified as a key adverse outcome linked to MWCNT exposure, and recommendations were made on the design of an in vitro assay that is predictive of the fibrotic potential of MWCNTs. While fibrosis takes weeks or months to develop in vivo, an in vitro test system may more rapidly predict fibrogenic potential by monitoring pro-fibrotic mediators (e.g., cytokines and growth factors). Therefore, the workshop discussions focused on the necessary specifications related to the development and evaluation of such an in vitro system. Recommendations were made for designing a system using lung-relevant cells co-cultured at the air-liquid interface to assess the pro-fibrogenic potential of aerosolized MWCNTs, while considering human-relevant dosimetry and NM life cycle transformations. The workshop discussions provided the fundamental design components of an air-liquid interface in vitro test system that will be subsequently expanded to the development of an alternative testing strategy to predict pulmonary toxicity and to generate data that will enable effective risk assessment of NMs. C1 [Clippinger, Amy J.; Sharma, Monita] PETA Int Sci Consortium Ltd, London, England. [Ahluwalia, Arti] Univ Pisa, Pisa, Italy. [Allen, David; Polk, William] Integrated Lab Syst Inc, Contractor Supporting Natl Toxicol Program Intera, Res Triangle Pk, NC USA. [Bonner, James C.] N Carolina State Univ, Raleigh, NC 27695 USA. [Casey, Warren] NTP Interagcy Ctr Evaluat Alternat Toxicol Method, Raleigh, NC USA. [Castranova, Vincent] W Virginia Univ, Morgantown, WV 26506 USA. [David, Raymond M.] BASF Corp, Florham Pk, NJ USA. [Halappanavar, Sabina] Hlth Canada, Environm & Radiat Hlth Sci Directorate, Ottawa, ON K1A 0L2, Canada. [Hotchkiss, Jon A.] Dow Chem Co USA, Midland, MI 48674 USA. [Jarabek, Annie M.] US EPA, Res Triangle Pk, NC 27711 USA. [Maier, Monika] Evon Ind AG, Hanau, Germany. [Rothen-Rutishauser, Barbara] Univ Fribourg, Adolphe Merkle Inst, CH-1700 Fribourg, Switzerland. [Sayes, Christie M.] Baylor Univ, Dept Environm Sci, Waco, TX 76798 USA. [Sayre, Phil] NanoRisk Analyt LLC, Washington, MD USA. [Stone, Vicki] Heriot Watt Univ, Edinburgh, Midlothian, Scotland. RP Clippinger, AJ (reprint author), PETA Int Sci Consortium Ltd, London, England. EM AmyJC@PISCLtd.org.uk RI Guenat, Heather/H-6528-2014 FU PETA International Science Consortium Ltd.; National Institute of Environmental Health Sciences (NIEHS) [HHSN27320140003C, HHSN273201500010C] FX The workshop was funded by the PETA International Science Consortium Ltd., and hosted by the United States Environmental Protection Agency. Integrated Laboratory Systems, Inc., staff provided technical support for National Toxicology Program (NTP) Interagency Center for the Evaluation of Alternative Toxicological Methods under National Institute of Environmental Health Sciences (NIEHS) contract HHSN27320140003C and HHSN273201500010C, but do not represent NIEHS, NTP, or the official positions of any Federal agency. NR 96 TC 4 Z9 4 U1 9 U2 17 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0340-5761 EI 1432-0738 J9 ARCH TOXICOL JI Arch. Toxicol. PD JUL PY 2016 VL 90 IS 7 BP 1769 EP 1783 DI 10.1007/s00204-016-1717-8 PG 15 WC Toxicology SC Toxicology GA DO3ZG UT WOS:000377720700021 PM 27121469 ER PT J AU Gonzalez-Mejia, AM Eason, TN Cabezas, H AF Gonzalez-Mejia, Alejandra M. Eason, Tarsha N. Cabezas, Heriberto TI System learning approach to assess sustainability and forecast trends in regional dynamics: The San Luis Basin study, Colorado, USA SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Artificial neural network; Fisher information; Forecast; Prediction; Baseline scenario; Sustainability; Regional system ID ARTIFICIAL NEURAL-NETWORKS; FISHER INFORMATION; INDICATORS; FUTURE AB This paper presents a methodology that combines the power of an Artificial Neural Network and Information Theory to forecast variables describing the condition of a regional system. The novelty and strength of this approach is in the application of Fisher information, a key method in Information Theory, to preserve trends in the historical data and prevent over fitting projections. The methodology was applied to demographic, environmental, food and energy consumption, and agricultural production in the San Luis Basin regional system in Colorado, U.S.A. These variables are important for tracking conditions in human and natural systems. However, available data are often so far out of date that they limit the ability to manage these systems. Results indicate that the approaches developed provide viable tools for forecasting outcomes with the aim of assisting management toward sustainable trends. This methodology is also applicable for modeling different scenarios in other dynamic systems. Published by Elsevier Ltd. C1 [Gonzalez-Mejia, Alejandra M.; Eason, Tarsha N.; Cabezas, Heriberto] US EPA, Sustainable Technol Div, Natl Risk Management Res Lab, Off Res & Dev, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Gonzalez-Mejia, Alejandra M.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Gonzalez-Mejia, Alejandra M.] Bangor Univ, Bangor, Gwynedd, Wales. [Gonzalez-Mejia, Alejandra M.] Ser Cymru Natl Res Network Low Carbon Energy & En, Sch Environm Nat Resources & Geog, Bangor, Gwynedd, Wales. RP Cabezas, H (reprint author), US EPA, Sustainable Technol Div, Natl Risk Management Res Lab, Off Res & Dev, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM gonzalez.alejandra@epa.gov; eason.tarsha@epa.gov; cabezas.heriberto@epa.gov NR 53 TC 1 Z9 1 U1 2 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD JUL PY 2016 VL 81 BP 1 EP 11 DI 10.1016/j.envsoft.2016.03.002 PG 11 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA DO4CJ UT WOS:000377729100001 ER PT J AU Martin, DM Hermoso, V Pantus, F Olley, J Linke, S Poff, NL AF Martin, David M. Hermoso, Virgilio Pantus, Francis Olley, Jon Linke, Simon Poff, N. LeRoy TI A proposed framework to systematically design and objectively evaluate non-dominated restoration tradeoffs for watershed planning and management SO ECOLOGICAL ECONOMICS LA English DT Article DE River restoration; Systems analysis; Decision making; Pareto efficiency; Multi-criteria decision analysis ID RIVER RESTORATION; ECOLOGICAL RESTORATION; DECISION-SUPPORT; REHABILITATION; UNCERTAINTY; CATCHMENT; BIODIVERSITY; QUEENSLAND; AUSTRALIA; SYSTEMS AB Human-driven alterations to freshwater ecosystems are leading to a global decline of river function and biodiversity. In our experience, managers want to be given many possible options to restore freshwater ecosystems that are workable within spatial, temporal, operational, and budgetary constraints of the system. Correspondingly, a new field for systematic river restoration planning has emerged through the use of well-established systems design concepts like multi-objective optimization and tradeoff analysis. In this article, we propose a decision framework for systematic river restoration planning where economic-environment systems design and tradeoff analyses are employed as a concurrent planning procedure before restoration interventions are implemented. Heuristic optimization and multi-criteria decision analysis methods are proposed to systematically design and objectively evaluate non-dominated economic-environment tradeoffs associated with restoration strategies within a watershed, and to provide a short-list of viable restoration alternatives to decision makers for negotiation and implementation. The proposed framework has the capacity to make science-based information and sophisticated decision support methods available for stakeholder deliberation. To illustrate the phases of the framework, we use a published case study of systematic restoration planning in South East Queensland, Australia. Published by Elsevier B.V. C1 [Martin, David M.; Poff, N. LeRoy] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Martin, David M.; Poff, N. LeRoy] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Martin, David M.] US EPA, Off Res & Dev, Atlantic Ecol Div, Narragansett, RI 02882 USA. [Hermoso, Virgilio] CTFC Forest Sci Ctr Catalonia, Solsona 25280, Lleida, Spain. [Hermoso, Virgilio; Pantus, Francis; Olley, Jon; Linke, Simon] Griffith Univ, Australian Rivers Inst, Nathan Campus,Kessels Rd, Nathan, Qld 4111, Australia. RP Martin, DM (reprint author), 27 Tarzwell Dr, Narragansett, RI 02882 USA. EM Martin.Davidm@epa.gov OI Hermoso, Virgilio/0000-0003-3205-5033 FU National Science Foundation IGERT [DGE-0966346]; Queensland government; SEQ Healthy Waterways Partnership, SEQ catchments; eWater Cooperative Research Centre; Ramon y Cajal fellowship [RYC-2013-13979]; ARC DECRA [DE130100565] FX This article was supported by the National Science Foundation IGERT Grant No. DGE-0966346 (DMM), The Queensland government, the SEQ Healthy Waterways Partnership, SEQ catchments, the eWater Cooperative Research Centre, the Ramon y Cajal fellowship RYC-2013-13979 (VH) and by ARC DECRA DE130100565 (SL). We thank one anonymous referee and the editor(s) who reviewed the manuscript. NR 49 TC 0 Z9 0 U1 9 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8009 EI 1873-6106 J9 ECOL ECON JI Ecol. Econ. PD JUL PY 2016 VL 127 BP 146 EP 155 DI 10.1016/j.ecolecon.2016.04.007 PG 10 WC Ecology; Economics; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology; Business & Economics GA DM9VF UT WOS:000376713100012 ER PT J AU Mirowsky, JE Dailey, LA Devlin, RB AF Mirowsky, Jaime E. Dailey, Lisa A. Devlin, Robert B. TI Differential expression of pro-inflammatory and oxidative stress mediators induced by nitrogen dioxide and ozone in primary human bronchial epithelial cells SO INHALATION TOXICOLOGY LA English DT Article DE In vitro; inflammation; nitrogen dioxide; oxidant; oxidative stress; ozone ID BRONCHOALVEOLAR LAVAGE FLUID; TIME-DEPENDENT CHANGES; IN-VITRO; ALVEOLAR MACROPHAGES; HEALTHY-SUBJECTS; CARBON-MONOXIDE; AMBIENT LEVELS; AIR-POLLUTION; PPM OZONE; EXPOSURE AB Context: NO2 and O-3 are ubiquitous air toxicants capable of inducing lung damage to the respiratory epithelium. Due to their oxidizing capabilities, these pollutants have been proposed to target specific biological pathways, but few publications have compared the pathways activated.Objective: This work will test the premise that NO2 and O-3 induce toxicity by activating similar cellular pathways.Methods: Primary human bronchial epithelial cells (HBECs, n=3 donors) were exposed for 2h at an air-liquid interface to 3ppm NO2, 0.75ppm O-3, or filtered air and harvested 1h post-exposure. To give an overview of pathways that may be influenced by each exposure, gene expression was measured using PCR arrays for toxicity and oxidative stress. Based on the results, genes were selected to quantify whether expression changes were changed in a dose- and time-response manner using NO2 (1, 2, 3, or 5ppm), O-3 (0.25, 0.50, 0.75, or 1.00ppm), or filtered air and harvesting 0, 1, 4 and 24h post-exposure.Results: Using the arrays, genes related to oxidative stress were highly induced with NO2 while expression of pro-inflammatory and vascular function genes was found subsequent to O-3. NO2 elicited the greatest HMOX1 response, whereas O-3 more greatly induced IL-6, IL-8 and PTGS2 expression. Additionally, O-3 elicited a greater response 1h post-exposure and NO2 produced a maximal response after 4h.Conclusion: We have demonstrated that these two oxidant gases stimulate differing mechanistic responses in vitro and these responses occur at dissimilar times. C1 [Mirowsky, Jaime E.] Univ N Carolina, Sch Med, Curriculum Toxicol, Chapel Hill, NC USA. [Mirowsky, Jaime E.] Univ N Carolina, Sch Med, Ctr Environm Med Asthma & Lung Biol, Chapel Hill, NC USA. [Dailey, Lisa A.; Devlin, Robert B.] US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Lab, Chapel Hill, NC USA. RP Mirowsky, JE (reprint author), US EPA, Human Studies Facil, 104 Mason Farm Rd, Chapel Hill, NC 27514 USA. EM mirowsky@email.unc.edu FU United States Environmental Protection Agency; EPA Cooperative Agreement with the Center for Environmental Medicine, Asthma, and Lung Biology at the University of North Carolina [CR83346301]; National Institutes of Environmental Health Sciences [T32ES007126] FX This work was supported by United States Environmental Protection Agency intramural funding, the EPA Cooperative Agreement with the Center for Environmental Medicine, Asthma, and Lung Biology at the University of North Carolina [CR83346301], and a National Institutes of Environmental Health Sciences T32 grant [T32ES007126]. The research described in this article has been reviewed by the National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, and approved for publication. Approval does not signify that the contents necessarily reflect the views and policies of the Agency, nor does the mention of trade names of commercial products constitute endorsement or recommendation for use. The authors report no declaration of interest. NR 50 TC 0 Z9 0 U1 6 U2 7 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0895-8378 EI 1091-7691 J9 INHAL TOXICOL JI Inhal. Toxicol. PD JUL PY 2016 VL 28 IS 8 BP 374 EP 382 DI 10.1080/08958378.2016.1185199 PG 9 WC Toxicology SC Toxicology GA DN4ME UT WOS:000377040300006 PM 27206323 ER PT J AU Karami, A Goh, YM Jahromi, MF Lazorchak, JM Abdullah, M Courtenay, SC AF Karami, Ali Goh, Yong-Meng Jahromi, Mohammad Faseleh Lazorchak, James M. Abdullah, Maha Courtenay, Simon C. TI Diploid and triploid African catfish (Clarias gariepinus) differ in biomarker responses to the pesticide chlorpyrifos SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Polyploidy; Morphometric; Hormonal; Biochemical; Gene expressions ID TROUT ONCORHYNCHUS-MYKISS; EUROPEAN SEA BASS; RAINBOW-TROUT; ACETYLCHOLINESTERASE INHIBITION; ENDOCRINE DISRUPTION; HEPATOSOMATIC INDEX; COMPARATIVE GROWTH; GENE-EXPRESSION; COHO SALMON; FISH AB The impacts of environmental stressors on polyploid organisms are largely unknown. This study investigated changes inmorphometric, molecular, and biochemical parameters in full-sibling diploid and triploid African catfish (Clarias gariepinus) in response to chlorpyrifos (CPF) exposures. Juvenile fish were exposed to three concentrations of CPF (mean measured mu g/L (SD): 9.71 (2.27), 15.7 (3.69), 31.21 (5.04)) under a static-renewal condition for 21 days. Diploid control groups had higher hepatosomatic index (HSI), plasma testosterone (T), and brain GnRH and cyp19a2 expression levels than triploids. In CPF-exposed groups, changes in HSI, total weight and length were different between the diploid and triploid fish. In contrast, condition factor did not alter in any of the treatments, while visceral-somatic index (VSI) changed only in diploids. In diploid fish, exposure to CPF did not change brain 11 beta-hsd2, ftz-f1, foxl2, GnRH or cyp19a2 mRNA levels, while reduced tph2 transcript levels compared to the control group. In contrast, 11 beta-hsd2 and foxl2 expression levels were changed in triploids following CPF exposures. In diploids, plasma T levels showed a linear dose-response reduction across CPF treatments correlating with liver weight and plasma total cholesterol concentrations. In contrast, no changes in plasma cholesterol and T concentrations were observed in triploids. Plasma cortisol and 17-beta estradiol (E-2) showed no response to CPF exposure in either ploidy. Results of this first comparison of biomarker responses to pesticide exposure in diploid and polyploid animals showed substantial differences between diploid and triploid C. gariepinus. (C) 2016 Elsevier B.V. All rights reserved. C1 [Karami, Ali] Univ Putra Malaysia, Fac Med & Hlth Sci, Lab Aquat Toxicol, Serdang 43400, Malaysia. [Goh, Yong-Meng] Univ Putra Malaysia, Fac Vet Med, Dept Vet Preclin Sci, Serdang 43400, Malaysia. [Jahromi, Mohammad Faseleh] Univ Putra Malaysia, Inst Trop Agr, Lab Anim Prod, Serdang 43400, Malaysia. [Lazorchak, James M.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [Abdullah, Maha] Univ Putra Malaysia, Fac Med & Hlth Sci, Immunol Lab, Serdang 43400, Malaysia. [Courtenay, Simon C.] Univ Waterloo, Canadian Rivers Inst, Canadian Water Network, Sch Environm Resources & Sustainabil, Waterloo, ON N2L 3G1, Canada. RP Karami, A (reprint author), Univ Putra Malaysia, Fac Med & Hlth Sci, Lab Aquat Toxicol, Serdang 43400, Malaysia. EM alikaramiv@gmail.com OI Lazorchak, James/0000-0002-7354-7571; Faseleh Jahromi, Mohammad/0000-0001-6035-5522 FU E-Science grant [01-02-05-SF0017]; Ministry of Science and Technology and Innovation (MOSTI), Government of Malaysia FX This work was funded in part by E-Science grant (01-02-05-SF0017), Ministry of Science and Technology and Innovation (MOSTI), Government of Malaysia. We acknowledge the following for their support: Samaneh Karbalaei, Samiaa Abdulwahid, Hazilawati Hamzah, Arman Addelan, Fatin binti Othman, Anthonysamy Arokiasamy, Marsitah Abdul Jalil, Rossalyna Rokmat, Hamzah Adam, and Sharmili Vidyadaran. Although this work was subjected to review by the US Environmental Protection Agency and approved for publication, it may not necessarily reflect official Agency policy nor should mention of commercial products be considered endorsement. NR 62 TC 2 Z9 2 U1 5 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUL 1 PY 2016 VL 557 BP 204 EP 211 DI 10.1016/j.scitotenv.2016.03.030 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK7VZ UT WOS:000375136200021 PM 26994807 ER PT J AU Gitipour, A Thiel, SW Scheckel, KG Tolaymat, T AF Gitipour, Alireza Thiel, Stephen W. Scheckel, Kirk G. Tolaymat, Thabet TI Anaerobic toxicity of cationic silver nanoparticles SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Silver nanoparticles; Anaerobic digestion; Dissolution; Stabilization mechanism; Anaerobic toxicity ID WASTE-WATER TREATMENT; SURFACE-CHARGE; ANTIBACTERIAL ACTIVITY; SEWAGE-SLUDGE; IMPACT; FATE; AGGREGATION; STABILITY; DIGESTION; KINETICS AB The microbial toxicity of silver nanoparticles (AgNPs) stabilized with different capping agents was compared to that of Ag+ under anaerobic conditions. Three AgNPs were investigated: (1) negatively charged citrate-coated AgNPs (citrate-AgNPs), (2) minimally charged polyvinylpyrrolidone coated AgNPs (PVP-AgNPs) and (3) positively charged branched polyethyleneimine coated AgNPs (BPEI-AgNPs). The AgNPs investigated in this experiment were similar in size (10-15 nm), spherical in shape, but varied in surface charge which ranged from highly negative to highly positive. While, at AgNPs concentrations lower than 5 mg L-1, the anaerobic decomposition process was not influenced by the presence of the nanoparticles, there was an observed impact on the diversity of the microbial community. At elevated concentrations (100 mg L-1 as silver), only the cationic BPEI-AgNPs demonstrated toxicity similar in magnitude to that of Ag+. Both citrate and PVP-AgNPs did not exhibit toxicity at the 100 mg L-1 as measured by biogas evolution. These findings further indicate the varying modes of action for nanoparticle toxicity and represent one of the few studies that evaluate end-of-life management concerns with regards to the increasing use of nanomaterials in our everyday life. These findings also highlight some of the concerns with a one size fits all approach to the evaluation of environmental health and safety concerns associated with the use of nanoparticles. Published by Elsevier B.V. C1 [Gitipour, Alireza; Thiel, Stephen W.] Univ Cincinnati, Biomed Chem & Environm Engn, Cincinnati, OH USA. [Scheckel, Kirk G.; Tolaymat, Thabet] US EPA, Off Res & Dev, 26 West Martin Luther King Dr, Cincinnati, OH 45224 USA. RP Tolaymat, T (reprint author), US EPA, Off Res & Dev, 26 West Martin Luther King Dr, Cincinnati, OH 45224 USA. EM tolaymat.thabet@epa.gov RI ID, MRCAT/G-7586-2011; OI Scheckel, Kirk/0000-0001-9326-9241 FU USEPA's Office of Research and Development Chemical Safety for Sustainability Program; Department of Energy; MRCAT member institutions; DOE Office of Science by Argonne National Laboratory [DE-AC02-06CH11357] FX This research was funded by the USEPA's Office of Research and Development Chemical Safety for Sustainability Program and has gone through the internal review process. Any mention of products or trade names does not constitute a recommendation for use by the USEPA. MRCAT operations are supported by the Department of Energy and the MRCAT member institutions. This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. NR 41 TC 2 Z9 2 U1 8 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUL 1 PY 2016 VL 557 BP 363 EP 368 DI 10.1016/j.scitotenv.2016.02.190 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK7VZ UT WOS:000375136200038 PM 27016684 ER PT J AU Walston, LJ Rollins, KE LaGory, KE Smith, KP Meyers, SA AF Walston, Leroy J., Jr. Rollins, Katherine E. LaGory, Kirk E. Smith, Karen P. Meyers, Stephanie A. TI A preliminary assessment of avian mortality at utility-scale solar energy facilities in the United States SO RENEWABLE ENERGY LA English DT Article DE Avian mortality; Utility-scale; Solar energy; Wind energy; Fossil fuels; Impact assessment ID WILDLIFE CONSERVATION; BIRD MORTALITY; FOSSIL-FUEL; WIND; COLLISIONS; REMOVAL AB Despite the benefits of reduced toxic and carbon emissions and a perpetual energy resource, there is potential for negative environmental impacts resulting from utility-scale solar energy (USSE) development. Although USSE development may represent an avian mortality source, there is little knowledge regarding the magnitude of these impacts in the context of other avian mortality sources. In this study we present a first assessment of avian mortality at USSE facilities through a synthesis of available avian monitoring and mortality information at existing USSE facilities. Using this information, we contextualize USSE avian mortality relative to other forms of avian mortality at 2 spatial scales: a regional scale (confined to southern California) and a national scale. Systematic avian mortality information was available for three USSE facilities in the southern California region. We estimated annual USSE-related avian mortality to be between 16,200 and 59,400 birds in the southern California region, which was extrapolated to between 37,800 and 138,600 birds for all USSE facilities across the United States that are either installed or under construction. We also discuss issues related to avian solar interactions that should be addressed in future research and monitoring programs. Published by Elsevier Ltd. C1 [Walston, Leroy J., Jr.; Rollins, Katherine E.; LaGory, Kirk E.; Smith, Karen P.; Meyers, Stephanie A.] Argonne Natl Lab, Div Environm Sci, 9700 S Cass Ave, Argonne, IL 60439 USA. [Meyers, Stephanie A.] US EPA, Reg 6, Dallas, TX USA. RP Walston, LJ (reprint author), Argonne Natl Lab, Div Environm Sci, 9700 S Cass Ave, Argonne, IL 60439 USA. EM lwalston@anl.gov FU Argonne, a DOE Office of Science laboratory [DE-AC02-06CH11357] FX This work was performed for the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, SunShot Initiative. This manuscript was created by UChicago Argonne, LLC, Operator of Argonne National Laboratory ("Argonne"). Argonne, a DOE Office of Science laboratory, is operated under Contract No. DE-AC02-06CH11357. The U.S. Government retains for itself, and others acting on its behalf, a paid-up nonexclusive, irrevocable worldwide license in said article to reproduce, prepare derivative works, distribute copies to the public, and perform publicly and display publicly, by or on behalf of the Government. NR 50 TC 0 Z9 0 U1 10 U2 31 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-1481 J9 RENEW ENERG JI Renew. Energy PD JUL PY 2016 VL 92 BP 405 EP 414 DI 10.1016/j.renene.2016.02.041 PG 10 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA DI3TI UT WOS:000373421000038 ER PT J AU Faulkner, BR Leibowitz, SG Canfield, TJ Groves, JF AF Faulkner, Barton R. Leibowitz, Scott G. Canfield, Timothy J. Groves, Justin F. TI Quantifying groundwater dependency of riparian surface hydrologic features using the exit gradient SO HYDROLOGICAL PROCESSES LA English DT Article DE exit gradient; basin fill aquifer; shallow groundwater management; wetland management; groundwater dependency of ecosystem services ID AGRICULTURAL LANDSCAPE; WATER AB Numerical groundwater flow models necessarily are limited to subsurface flow evaluation. It is of interest, however, to examine the possibility that, for unconfined aquifer systems, they could be used to proportionately measure the magnitude of seepage they estimate when these aquifers intersect the landscape surface. Our goal in this study was to determine the degree to which an unconfined groundwater model can estimate run-off or seepage at the land surface during winter time wet season conditions, as well as in the dry season, when evapotranspiration is a major part of the water balance, using a lowland basin-fill example study area in the Pacific Northwest. The exit gradient is a metric describing the potential for vertical seepage at the landscape surface. We investigated the spatial relationship of mapped surface features, such as wetlands, streams and ponds, to the model-predicted mapped exit gradient. We found that areas mapped as wetlands had positive exit gradients. During the wet season, modelled exit gradients predicted seepage throughout extensive areas of the groundwater shed, extending far beyond mapped wetland areas (355% increase), associated with previously observed increases in nitrate-nitrogen in streams in wet season. During the dry season, exit gradients spatially corresponded with wetland areas. The increase in in-stream nitrogen corresponds with shorter residence times in carbon-rich wetland zones because of the onset of saturation overland flow. We present results that suggest that the exit gradient could be a useful concept in examining the groundwater-surface water linkage that is often under represented physically in watershed flow models. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Faulkner, Barton R.; Canfield, Timothy J.; Groves, Justin F.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, 919 Kerr Res Dr, Ada, OK 74820 USA. [Leibowitz, Scott G.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, 200 SW 35th St, Corvallis, OR 97333 USA. RP Faulkner, BR (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, 919 Kerr Res Dr, Ada, OK 74820 USA. EM faulkner.bart@epa.gov FU US Environmental Protection Agency FX The authors thank Jim Wigington and Jana Compton of the USEPA National Health and Environmental Effects Research Laboratory, Western Ecology Division, for their support and useful input for this work. We also thank Steve Vandegrift of the USEPA National Risk Management Research Laboratory, Ground Water and Ecosystems Restoration Research Division, for his helpful initial review and comments for the manuscript. The US Environmental Protection Agency funded and managed the research described here. It has been subjected to the Agency's administrative review and approved for publication. NR 27 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD JUN 30 PY 2016 VL 30 IS 13 BP 2167 EP 2177 DI 10.1002/hyp.10766 PG 11 WC Water Resources SC Water Resources GA DR2MW UT WOS:000379739600015 ER PT J AU Spanbauer, TL Allen, CR Angeler, DG Eason, T Fritz, SC Garmestani, AS Nash, KL Stone, JR Stow, CA Sundstrom, SM AF Spanbauer, Trisha L. Allen, Craig R. Angeler, David G. Eason, Tarsha Fritz, Sherilyn C. Garmestani, Ahjond S. Nash, Kirsty L. Stone, Jeffery R. Stow, Craig A. Sundstrom, Shana M. TI Body size distributions signal a regime shift in a lake ecosystem SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE palaeoecology; regime shift; climate change; thresholds; body size; resilience ID HABITAT STRUCTURE; DISCONTINUITIES; ORGANIZATION; RESILIENCE; PATTERNS; DROUGHT; ECOLOGY; DIATOM; TIME AB Communities of organisms, from mammals to microorganisms, have discontinuous distributions of body size. This pattern of size structuring is a conservative trait of community organization and is a product of processes that occur at multiple spatial and temporal scales. In this study, we assessed whether body size patterns serve as an indicator of a threshold between alternative regimes. Over the past 7000 years, the biological communities of Foy Lake (Montana, USA) have undergone a major regime shift owing to climate change. We used a palaeoecological record of diatom communities to estimate diatom sizes, and then analysed the discontinuous distribution of organism sizes over time. We used Bayesian classification and regression tree models to determine that all time intervals exhibited aggregations of sizes separated by gaps in the distribution and found a significant change in diatom body size distributions approximately 150 years before the identified ecosystem regime shift. We suggest that discontinuity analysis is a useful addition to the suite of tools for the detection of early warning signals of regime shifts. C1 [Spanbauer, Trisha L.] US EPA, Natl Res Council, Cincinnati, OH 45268 USA. [Eason, Tarsha; Garmestani, Ahjond S.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Spanbauer, Trisha L.; Fritz, Sherilyn C.] Univ Nebraska, Dept Earth & Atmospher Sci, Lincoln, NE 68588 USA. [Spanbauer, Trisha L.; Fritz, Sherilyn C.] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA. [Allen, Craig R.] Univ Nebraska, US Geol Survey, Nebraska Cooperat Fish & Wildlife Res Unit, Sch Nat Resources, Lincoln, NE 68583 USA. [Sundstrom, Shana M.] Univ Nebraska, Nebraska Cooperat Fish & Wildlife Res Unit, Sch Nat Resources, Lincoln, NE 68583 USA. [Angeler, David G.] Swedish Univ Agr Sci, Dept Aquat Sci & Assessment, POB 7050, S-75007 Uppsala, Sweden. [Nash, Kirsty L.] James Cook Univ, Australian Res Council, Ctr Excellence Coral Reef Studies, Townsville, Qld 4811, Australia. [Nash, Kirsty L.] Univ Tasmania, Inst Marine & Antarctic Studies, Ctr Marine Socioecol, Hobart, Tas 7000, Australia. [Stone, Jeffery R.] Indiana State Univ, Dept Earth & Environm Syst, Terre Haute, IN 47809 USA. [Stow, Craig A.] NOAA, Great Lakes Environm Res Lab, Ann Arbor, MI 48108 USA. RP Spanbauer, TL (reprint author), US EPA, Natl Res Council, Cincinnati, OH 45268 USA.; Spanbauer, TL (reprint author), Univ Nebraska, Dept Earth & Atmospher Sci, Lincoln, NE 68588 USA.; Spanbauer, TL (reprint author), Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA. EM spanbauer.trisha@epa.gov OI Nash, Kirsty/0000-0003-0976-3197 FU United States Geological Survey's John Wesley Powell Center for Analysis and Synthesis; August T. Larsson Foundation of the Swedish University of Agricultural Sciences; NSF's Integrative Graduate Education and Research Traineeship (IGERT) programme (NSF) [0903469]; NSF's Sedimentary Geology and Palaeobiology programme (NSF) [1251678]; Swedish Research Council VR [2014-5828]; Swedish Research Council Formas [2014-1193]; University of Nebraska; National Research Council FX This manuscript was conceived at the Managing for Resilience Working Group, funded by the United States Geological Survey's John Wesley Powell Center for Analysis and Synthesis. This work was supported, in part, by the August T. Larsson Foundation of the Swedish University of Agricultural Sciences, the NSF's Integrative Graduate Education and Research Traineeship (IGERT) programme (NSF no. 0903469), the Sedimentary Geology and Palaeobiology programme (NSF no. 1251678), the Swedish Research Councils VR (2014-5828) and Formas (2014-1193). A University of Nebraska Presidential Graduate Fellowship and a National Research Council Research Associateship also provided support for this project. NR 42 TC 0 Z9 0 U1 8 U2 8 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD JUN 29 PY 2016 VL 283 IS 1833 AR 20160249 DI 10.1098/rspb.2016.0249 PG 6 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA DT1MR UT WOS:000381247300017 ER PT J AU Li, Y Schichtel, BA Walker, JT Schwede, DB Chen, X Lehmann, CMB Puchalski, MA Gay, DA Collett, JL AF Li, Yi Schichtel, Bret A. Walker, John T. Schwede, Donna B. Chen, Xi Lehmann, Christopher M. B. Puchalski, Melissa A. Gay, David A. Collett, Jeffrey L., Jr. TI REPLY TO LIU ET AL.: On the importance of US deposition of nitrogen dioxide, coarse particle nitrate, and organic nitrogen SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Letter C1 [Li, Yi; Collett, Jeffrey L., Jr.] Colorado State Univ, Dept Atmospher Sci, Ft Collins, CO 80523 USA. [Schichtel, Bret A.] Colorado State Univ, Natl Pk Serv, Cooperat Inst Res Atmosphere, Ft Collins, CO 80523 USA. [Walker, John T.; Chen, Xi] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, Res Triangle Pk, NC 27711 USA. [Schwede, Donna B.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, Res Triangle Pk, NC 27711 USA. [Lehmann, Christopher M. B.; Gay, David A.] Univ Illinois, Natl Atmospher Deposit Program, Champaign, IL 61820 USA. [Puchalski, Melissa A.] US EPA, Clean Air Markets Div, Washington, DC 20460 USA. RP Collett, JL (reprint author), Colorado State Univ, Dept Atmospher Sci, Ft Collins, CO 80523 USA. EM collett@atmos.colostate.edu RI Collett, Jeffrey/F-2862-2010; Li, Yi/N-4000-2015 OI Collett, Jeffrey/0000-0001-9180-508X; Li, Yi/0000-0002-6022-9136 NR 5 TC 0 Z9 0 U1 9 U2 12 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUN 28 PY 2016 VL 113 IS 26 BP E3592 EP E3593 DI 10.1073/pnas.1607738113 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ2LI UT WOS:000379033400002 PM 27307446 ER PT J AU Holden, PA Gardea-Torresdey, JL Klaessig, F Turco, RF Mortimer, M Hund-Rinke, K Hubal, EAC Avery, D Barcelo, D Behra, R Cohen, Y Deydier-Stephan, L Ferguson, PL Fernandes, TF Harthorn, BH Henderson, WM Hoke, RA Hristozov, D Johnston, JM Kane, AB Kapustka, L Keller, AA Lenihan, HS Lovell, W Murphy, CJ Nisbet, RM Petersen, EJ Salinas, ER Scheringer, M Sharma, M Speed, DE Sultan, Y Westerhoff, P White, JC Wiesner, MR Wong, EM Xing, BS Horan, MS Godwin, HA Nel, AE AF Holden, Patricia A. Gardea-Torresdey, Jorge L. Klaessig, Fred Turco, Ronald F. Mortimer, Monika Hund-Rinke, Kerstin Hubal, Elaine A. Cohen Avery, David Barcelo, Damia Behra, Renata Cohen, Yoram Deydier-Stephan, Laurence Ferguson, P. Lee Fernandes, Teresa F. Harthorn, Barbara Herr Henderson, W. Matthew Hoke, Robert A. Hristozov, Danail Johnston, John M. Kane, Agnes B. Kapustka, Larry Keller, Arturo A. Lenihan, Hunter S. Lovell, Wess Murphy, Catherine J. Nisbet, Roger M. Petersen, Elijah J. Salinas, Edward R. Scheringer, Martin Sharma, Monita Speed, David E. Sultan, Yasir Westerhoff, Paul White, Jason C. Wiesner, Mark R. Wong, Eva M. Xing, Baoshan Horan, Meghan Steele Godwin, Hilary A. Nel, Andre E. TI Considerations of Environmentally Relevant Test Conditions for Improved Evaluation of Ecological Hazards of Engineered Nanomaterials SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Review ID WALLED CARBON NANOTUBES; TITANIUM-DIOXIDE NANOPARTICLES; WASTE-WATER TREATMENT; CERIUM OXIDE NANOPARTICLES; COATED SILVER NANOPARTICLES; NEAR-INFRARED FLUORESCENCE; SOIL BACTERIAL COMMUNITIES; ECOTOXICITY TEST METHODS; AQUATIC TOXICITY TESTS; PERSONAL CARE PRODUCTS AB Engineered nanomaterials (ENMs) are increasingly entering the environment with uncertain consequences including potential ecological effects. Various research communities view differently whether ecotoxicological testing of ENMs should be conducted using environmentally relevant concentrations where observing outcomes is difficult versus higher ENM doses, where responses are observable. What exposure conditions are typically used in assessing ENM hazards to populations? What conditions are used to test ecosystem-scale hazards? What is known regarding actual ENMs in the environment, via measurements or modeling simulations? How should exposure conditions, ENM transformation, dose, and body burden be used in interpreting biological and computational findings for assessing risks? These questions were addressed in the context of this critical review. As a result, three main recommendations emerged. First, researchers should improve ecotoxicology of ENMs by choosing test end points, duration, and study conditions-including ENM test concentrations that align with realistic exposure scenarios. Second, testing should proceed via tiers with iterative feedback that informs experiments at other levels of biological organization. Finally, environmental realism in ENM hazard assessments should involve greater coordination among ENM quantitative analysts, exposure modelers, and ecotoxicologists, across government, industry, and academia. C1 [Holden, Patricia A.; Mortimer, Monika; Keller, Arturo A.; Lenihan, Hunter S.] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA. [Holden, Patricia A.; Gardea-Torresdey, Jorge L.; Klaessig, Fred; Mortimer, Monika; Avery, David; Cohen, Yoram; Harthorn, Barbara Herr; Keller, Arturo A.; Lenihan, Hunter S.; Nisbet, Roger M.; Horan, Meghan Steele; Godwin, Hilary A.; Nel, Andre E.] Univ Calif Los Angeles, UC CEIN, Los Angeles, CA 90095 USA. [Gardea-Torresdey, Jorge L.] Univ Texas El Paso, Environm Sci & Engn PhD Program, Dept Chem, El Paso, TX 79968 USA. [Klaessig, Fred] Penn Bio Nano Syst, Doylestown, PA 18901 USA. [Turco, Ronald F.] Purdue Univ, Coll Agr, Soil Microbiol Lab, W Lafayette, IN 47907 USA. [Mortimer, Monika] NICPB, Lab Environm Toxicol, Akad Tee 23, EE-12618 Tallinn, Estonia. [Hund-Rinke, Kerstin] Fraunhofer Inst Mol Biol & Appl Ecol, D-57392 Schmallenberg, Germany. [Hubal, Elaine A. Cohen] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Barcelo, Damia] Inst Environm Assessment & Water Res IDAEA CSIC, Dept Environm Chem, Barcelona 08034, Spain. [Barcelo, Damia] Univ Girona, ICRA, Parc Cient & Tecnol, Girona 17003, Spain. [Behra, Renata] Eawag, Swiss Fed Inst Aquat Sci & Technol, CH-8600 Dubendorf, Switzerland. [Behra, Renata] ETH, Inst Biogeochem & Pollutant Dynam, CH-8092 Zurich, Switzerland. [Cohen, Yoram; Godwin, Hilary A.; Nel, Andre E.] Univ Calif Los Angeles, Calif NanoSyst Inst, Chem & Biomol Engn Dept, Los Angeles, CA 90095 USA. [Deydier-Stephan, Laurence] European Chem Agcy ECHA, Annankatu 18, Helsinki 00121, Finland. [Ferguson, P. Lee] Duke Univ, Dept Civil & Environm Engn, CEINT, Durham, NC 27708 USA. [Fernandes, Teresa F.] Heriot Watt Univ, Edinburgh EH14 4AS, Midlothian, Scotland. [Harthorn, Barbara Herr] Univ Calif Santa Barbara, Dept Anthropol, Ctr Nanotechnol Soc, Santa Barbara, CA 93106 USA. [Henderson, W. Matthew; Johnston, John M.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Athens, GA 30605 USA. [Hoke, Robert A.] EI du Pont de Nemours & Co, Newark, DE 19711 USA. [Hristozov, Danail] Univ Ca Foscari Venice, Dept Environm Sci Informat & Stat, I-30123 Venice, Italy. [Kane, Agnes B.] Brown Univ, Dept Pathol & Lab Med, Providence, RI 02912 USA. [Kapustka, Larry] LK Consultancy, Turner Valley, AB T0L 2A0, Canada. [Lovell, Wess] Vive Crop Protect Inc, Toronto, ON M5G 1L6, Canada. [Murphy, Catherine J.] Univ Illinois, Dept Chem, Urbana, IL 61801 USA. [Nisbet, Roger M.] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA. [Petersen, Elijah J.] NIST, Biosyst & Biomat Div, Mat Measurement Lab, Gaithersburg, MD 20899 USA. [Salinas, Edward R.] BASF SE, Expt Toxicol & Ecol, D-67056 Ludwigshafen, Germany. [Scheringer, Martin] ETH, Inst Chem & Bioengn, CH-8093 Zurich, Switzerland. [Sharma, Monita] PETA Int Sci Consortium Ltd, London N1 9RL, England. [Speed, David E.] Corp EHS, Globalfoundries, Hopewell Jct, NY 12533 USA. [Sultan, Yasir] Environm & Climate Change Canada, Gatineau, PQ K1A 0H3, Canada. [Westerhoff, Paul] Arizona State Univ, Sch Sustainable Engn & Built Environm, Tempe, AZ 85287 USA. [White, Jason C.] Connecticut Agr Expt Stn, Dept Analyt Chem, New Haven, CT 06504 USA. [Wong, Eva M.] US EPA, Off Pollut Prevent & Tox, Washington, DC 20460 USA. [Xing, Baoshan] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA. [Godwin, Hilary A.] Univ Calif Los Angeles, Fielding Sch Publ Hlth, Dept Environm Hlth Sci, Los Angeles, CA 90095 USA. [Godwin, Hilary A.] Univ Calif Los Angeles, Inst Environm & Sustainabil, Los Angeles, CA 90095 USA. [Nel, Andre E.] Univ Calif Los Angeles, Dept Med, Div NanoMed, Los Angeles, CA 90095 USA. RP Holden, PA (reprint author), Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA.; Holden, PA (reprint author), Univ Calif Los Angeles, UC CEIN, Los Angeles, CA 90095 USA. EM holden@bren.ucsb.edu RI Nisbet, Roger/B-6951-2014; Ferguson, Lee/A-5501-2013; Mortimer, Monika/A-2593-2013; OI Murphy, Catherine/0000-0001-7066-5575; Mortimer, Monika/0000-0001-9008-521X; Hristozov, Danail/0000-0002-2386-7366; Fernandes, Teresa/0000-0002-8541-598X FU National Science Foundation; U.S. Environmental Protection Agency [DBI-1266377] FX Funding was from the National Science Foundation and the U.S. Environmental Protection Agency under Cooperative Agreement DBI-1266377. Any opinions, findings, and conclusions are those of the authors and do not necessarily reflect the views of the National Science Foundation, the U.S. Environmental Protection Agency, or the National Institute of Standards and Technology. Although this document has been reviewed in accordance with U.S. Environmental Protection Agency policy and approved for publication, the views are those of the authors and may not necessarily reflect official Agency policy. Mention of trade names or commercial products should not be interpreted as endorsement or recommendation for use. We acknowledge the helpful comments of three anonymous reviewers. NR 299 TC 16 Z9 16 U1 64 U2 115 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 21 PY 2016 VL 50 IS 12 BP 6124 EP 6145 DI 10.1021/acs.est.6b00608 PG 22 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DP4ML UT WOS:000378469900004 PM 27177237 ER PT J AU Wood, A Blackhurst, M Garland, JL Lawler, DF AF Wood, Alison Blackhurst, Michael Garland, Jay L. Lawler, Desmond F. TI Incentivizing Decentralized Sanitation: The Role of Discount Rates SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID WASTE-WATER MANAGEMENT; PURCHASE; COST; DURABLES; SYSTEMS AB In adoption decisions for decentralized sanitation technologies, two decision makers are involved: the public utility and the individual homeowner. Standard life cycle cost is calculated from the perspective of the utility, which uses a market-based discount rate in these calculations. However, both decision-makers must be considered, including their differing perceptions of the time trade-offs inherent in a stream of costs and benefits. This study uses the discount rate as a proxy for these perceptions and decision-maker preferences. The results in two case studies emphasize the dependence on location of such analyses. Falmouth, Massachusetts, appears to be a good candidate for incentivizing decentralized sanitation while the Allegheny County Sanitary Authority service area in Pennsylvania appears to have no need for similar incentives. This method can be applied to any two-party decision in which the parties are expected to have different discount rates. C1 [Wood, Alison; Lawler, Desmond F.] Univ Texas Austin, Dept Civil Architectural & Environm Engn, 301 E Dean Keeton St C8600, Austin, TX 78712 USA. [Blackhurst, Michael] Univ Pittsburgh, Univ Ctr Social & Urban Res, 3343 Forbes Ave, Pittsburgh, PA 15260 USA. [Garland, Jay L.] US EPA, Off Res & Dev, Syst Exposure Div, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. RP Wood, A (reprint author), Univ Texas Austin, Dept Civil Architectural & Environm Engn, 301 E Dean Keeton St C8600, Austin, TX 78712 USA. EM alisonwood@gmail.com FU USEPA National Network for Environmental Management Studies Fellowship Program [U-91755801-0] FX We acknowledge the USEPA National Network for Environmental Management Studies Fellowship Program (Grant U-91755801-0) for funding the lead author. The authors also thank Jennifer Cashdollar and Cissy Ma for their support in this work, and all the individuals who provided information. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 40 TC 0 Z9 0 U1 6 U2 6 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 21 PY 2016 VL 50 IS 12 BP 6146 EP 6153 DI 10.1021/acs.est.6b00385 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DP4ML UT WOS:000378469900005 PM 27183382 ER PT J AU Maestre, JP Wahman, DG Speitel, GE AF Maestre, Juan P. Wahman, David G. Speitel, Gerald E., Jr. TI Monochloramine Cometabolism by Mixed-Culture Nitrifiers under Drinking Water Conditions SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID NITROSOMONAS-EUROPAEA; COMPLETE NITRIFICATION; DISTRIBUTION-SYSTEMS; REACTION SCHEME; TRIHALOMETHANES; HYDROXYLAMINE; CHLORAMINES; OXIDATION; BACTERIA; NITRITE AB Chloramines are the second most used secondary disinfectant by United States water utilities. However, chloramination may promote nitrifying bacteria. Recently, monochloramine cometabolisin by the pure culture ammonia-oxidizing bacteria, Nitrosomonas europaea, was shown to increase monochloramine demand. The current research investigated monochloramine cometabolism by nitrifying mixed cultures grown under more relevant drinking water conditions and harvested from sand-packed reactors before conducting suspended growth batch kinetic experiments. Four types of batch kinetic experiments were conducted: (1) positive controls to estimate ammonia kinetic parameters, (2) negative controls to account for biomass reactivity, (3) utilization associated product (UAP) controls to account for UAP reactivity, and (4) cometabolism experiments to estimate cometabolism kinetic parameters. Kinetic parameters were estimated in AQUASIM with a simultaneous fit to the experimental data. Cometabolism kinetics were best described by a first-order model. Monochloramine cometabolism kinetics were similar to those of ammonia metabolism, and monochloramine cometabolism accounted for 30% of the observed monochloramine loss. These results demonstrated that monochloramine cometabolism occurred in mixed cultures similar to those found in drinking water distribution systems; therefore, monochloramine cometabolism may be a significant contribution to monochloramine loss during nitrification episodes in drinking water distribution systems. C1 [Maestre, Juan P.; Speitel, Gerald E., Jr.] Univ Texas Austin, Dept Civil Architectural & Environm Engn, 301 East Dean Keeton St,Stop C2100, Austin, TX 78712 USA. [Wahman, David G.] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. RP Speitel, GE (reprint author), Univ Texas Austin, Dept Civil Architectural & Environm Engn, 301 East Dean Keeton St,Stop C2100, Austin, TX 78712 USA. EM speitel@mail.utexas.edu FU Water Research Foundation, Denver, CO, USA FX This research was funded by the Water Research Foundation, Denver, CO, USA. NR 34 TC 0 Z9 0 U1 10 U2 15 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 21 PY 2016 VL 50 IS 12 BP 6240 EP 6248 DI 10.1021/acs.est.5b05641 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DP4ML UT WOS:000378469900016 PM 27196729 ER PT J AU Roy, A Sonntag, D Cook, R Yanca, C Schenk, C Choi, Y AF Roy, Anirban Sonntag, Darrell Cook, Richard Yanca, Catherine Schenk, Charles Choi, Yunsoo TI Effect of Ambient Temperature on Total Organic Gas Speciation Profiles from Light-Duty Gasoline Vehicle Exhaust SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID SURFACE OZONE; TEXAS; NOX AB Total organic gases (TOG) emissions from motor vehicles include air toxic compounds and contribute to formation of ground-level ozone and secondary organic aerosol (SOA). These emissions are known to be affected by temperature; however previous studies have typically focused only on the temperature dependence of total emission factors and select toxic compounds. This study builds on the previous research by performing an evaluation of a comprehensive set of gas-phase organic compounds present in gasoline motor vehicle exhaust. A fleet of five vehicles using port fuel injection engine technology and running on E10 fuel was tested. Overall, three temperatures (0, 20, and 75 degrees F; or -18, -7, and 24 degrees C), two driving conditions (urban-FTP75 and aggressive driving-US06) and 161 compounds were evaluated; the emissions distributions were used to construct speciation profiles for each driving cycle and temperature. Overall, the speciation results indicated a significant increase in alkane and methane content, and decrease in alcohol, aldehyde and ketone content with decreasing temperature. These were verified using a statistical significance test. The fraction and composition of Mobile Source Air Toxics (MSATs) were significantly affected by temperature for both driving cycles. The ozone forming potentials of these profiles were evaluated using the maximum incremental reactivity (MIR) scale. Aromatic content was predicted to be a major driver behind the ozone forming potentials. Additionally, the decreasing ozone potential could be attributed to increased methane fractions with increasing temperature. C1 [Roy, Anirban; Choi, Yunsoo] Univ Houston, Dept Earth & Atmospher Sci, Houston, TX 77004 USA. [Sonntag, Darrell; Cook, Richard; Yanca, Catherine; Schenk, Charles] US EPA, Off Transportat & Air Qual, Ann Arbor, MI 48105 USA. RP Choi, Y (reprint author), Univ Houston, Dept Earth & Atmospher Sci, Houston, TX 77004 USA. EM ychoi23@central.uh.edu FU Texas Air Research Centre (TARC); Air Quality Research Program (AQRP) through the University of Texas at Austin FX This work was supported by the Texas Air Research Centre (TARC) and the Air Quality Research Program (AQRP) through the University of Texas at Austin. This paper may not necessarily reflect the views of the USEPA and no official endorsement should be inferred. We thank Joseph McDonald at the Office of Research and Development at Cincinnati, Ohio for his inputs on the text. NR 33 TC 0 Z9 0 U1 13 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 21 PY 2016 VL 50 IS 12 BP 6565 EP 6573 DI 10.1021/acs.est.6b01081 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DP4ML UT WOS:000378469900052 PM 27203618 ER PT J AU Millers, DB Ghio, AJ Karoly, ED Bell, LN Snow, SJ Madden, MC Soukup, J Cascio, WE Gilmour, MI Kodavanti, UP AF Millers, Desinia B. Ghio, Andrew J. Karoly, Edward D. Bell, Lauren N. Snow, Samantha J. Madden, Michael C. Soukup, Joleen Cascio, Wayne E. Gilmour, M. Ian Kodavanti, Urmila P. TI Ozone Exposure Increases Circulating Stress Hormones and Lipid Metabolites in Humans SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article DE air pollution; stress response; lipid mediators; fatty acids ID FINE PARTICULATE MATTER; AIR-POLLUTION EXPOSURE; LONG-TERM EXPOSURE; BROWN-NORWAY RATS; DIABETES-MELLITUS; EPITHELIAL-CELLS; AMBIENT LEVELS; GLUCOSE; RISK; GLUCOCORTICOIDS AB Rationale: Air pollution has been associated with increased prevalence of type 2 diabetes; however, the mechanisms remain unknown. We have shown that acute ozone exposure in rats induces release of stress hormones, hyperglycemia, leptinemia, and glucose intolerance that are associated with global changes in peripheral glucose, lipid, and amino acid metabolism. Objectives: To examine ozone-induced metabolic derangement in humans using serum metabolomic assessment, establish human-to-rodent coherence, and identify novel nonprotein biomarkers. Methods: Serum samples were obtained from a crossover clinical study that-included two clinic visits (n = 24 each) where each subject was blindly exposed in the morning to either filtered air or 0.3 parts per million ozone for 2 hours during 15-minute on-off exercise. Serum samples collected within 1 hour after exposure were assessed for changes in metabolites using a metabolomic approach. Measurements and-Main Results: Metabolomic analysis revealed that ozone exposure markedly increased serum cortisol and corticosterone together with increases in monoacylglycerol, glycerol, and medium- and long-chain free fatty acids, reflective of lipid mobilization and catabolism. Additionally, ozone exposure increased serum lysolipids, potentially originating from membrane lipid breakdown. Ozone exposure also increased circulating mitochondrial beta-oxidation-derived metabolites, such as acylcarnitines, together with increases in the ketone body 3-hydroxybutyrate. These changes suggested saturation of beta-oxidation by ozone in exercising humans. Conclusions: As in rodents, acute ozone exposure increased stress hormones and globally altered peripheral lipid metabolism in humans, likely through activation of a neurohormonally mediated stress response pathway. The metabolomic assessment revealed new biomarkers and allowed for establishment of rodent-to-human coherence. C1 [Millers, Desinia B.] Univ N Carolina, Curriculum Toxicol, Chapel Hill, NC USA. [Ghio, Andrew J.; Snow, Samantha J.; Madden, Michael C.; Soukup, Joleen; Cascio, Wayne E.; Gilmour, M. Ian; Kodavanti, Urmila P.] US EPA, Natl Hlth & Environm Effects Res Lab, Environm Publ Hlth Div, Res Triangle Pk, NC 27711 USA. [Karoly, Edward D.; Bell, Lauren N.] Metabolon Inc, Durham, NC USA. RP Kodavanti, UP (reprint author), US EPA, NHEERL, B105-04, Res Triangle Pk, NC 27711 USA. EM kodavanti.urmila@epa.gov OI Snow, Samantha/0000-0003-1812-8582 FU U.S. Environmental Protection Agency-University of North Carolina [CR-83515201]; UNC-NIEHS Toxicology Training Grant [T32 ES007126]; HHMI FX Supported by the U.S. Environmental Protection Agency-University of North Carolina Cooperative Trainee Agreement (#CR-83515201) and UNC-NIEHS Toxicology Training Grant (T32 ES007126). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. D.B.M. was a recipient of HHMI-funded UNC Program in Translational Medicine award. NR 47 TC 4 Z9 4 U1 4 U2 6 PU AMER THORACIC SOC PI NEW YORK PA 25 BROADWAY, 18 FL, NEW YORK, NY 10004 USA SN 1073-449X EI 1535-4970 J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD JUN 15 PY 2016 VL 193 IS 12 BP 1382 EP 1391 DI 10.1164/rccm.201508-1599OC PG 10 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA DO8FF UT WOS:000378017300014 PM 26745856 ER PT J AU Gullett, BK Hays, MD Tabor, D Vander Wal, R AF Gullett, Brian K. Hays, Michael D. Tabor, Dennis Vander Wal, Randy TI Characterization of the particulate emissions from the BP Deepwater Horizon surface oil burns SO MARINE POLLUTION BULLETIN LA English DT Article DE Oil; Burn; Emissions; Deepwater Horizon; Soot; Characterization ID SPILL; FIRES; SOOT; COMBUSTION; PARTICLE; AEROSOLS AB Sampling of the smoke plumes from the BP Deepwater Horizon surface oil burns led to the unintentional collection of soot particles on the sail of an instrument-bearing, tethered aerostat. This first-ever plume sampling from oil burned at an actual spill provided an opportunistic sample from which to characterize the particles' chemical properties for polycyclic aromatic hydrocarbons (PAHs), organic carbon, elemental carbon, metals, and polychlorinated dibenzodioxins/dibenzofurans (PCDDs/PCDFs) and physical properties for size and nanostructure. Thermal-optical analyses indicated that the particulate matter was 93% carbon with 82% being refractory elemental carbon. PAHs accounted for roughly 68 mu/g of the PM filter mass and 5 mg/kg oil burned, much lower than earlier laboratory based studies. Microscopy indicated that the soot is distinct from more common soot by its aggregate size, primary particle size, and nanostructure. PM-bound metals were largely unremarkable but PCDD/PCDF formation was observed, contrary to other's findings. Levels of lighter PCDD/PCDF and PAH compounds were reduced compared to historical samples, possibly due to volatilization or photo-oxidation. Published by Elsevier Ltd. C1 [Gullett, Brian K.; Hays, Michael D.; Tabor, Dennis] US EPA, Off Res & Dev E343 04, Res Triangle Pk, NC 27711 USA. [Vander Wal, Randy] Penn State Univ, John & Willie Leone Family Dept Energy, University Pk, PA 16802 USA. [Vander Wal, Randy] Penn State Univ, Dept Mineral Engn, University Pk, PA 16802 USA. [Vander Wal, Randy] Penn State Univ, EMS Energy Inst, University Pk, PA 16802 USA. RP Gullett, BK (reprint author), US EPA, Off Res & Dev E343 04, Res Triangle Pk, NC 27711 USA. EM Gullett.brian@epa.gov FU U.S. Environmental Protection Agency (US EPA) FX This work was entirely funded by the U.S. Environmental Protection Agency (US EPA). The views expressed in this article are those of the author(s) and do not necessarily represent the views or policies of the US EPA. NR 32 TC 0 Z9 0 U1 5 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0025-326X EI 1879-3363 J9 MAR POLLUT BULL JI Mar. Pollut. Bull. PD JUN 15 PY 2016 VL 107 IS 1 BP 216 EP 223 DI 10.1016/j.marpolbul.2016.03.069 PG 8 WC Environmental Sciences; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DO4BL UT WOS:000377726700038 PM 27084200 ER PT J AU Akob, DM Mumford, AC Orem, W Engle, MA Klinges, JG Kent, DB Cozzarelli, IM AF Akob, Denise M. Mumford, Adam C. Orem, William Engle, Mark A. Klinges, J. Grace Kent, Douglas B. Cozzarelli, Isabelle M. TI Wastewater Disposal from Unconventional Oil and Gas Development Degrades Stream Quality at a West Virginia Injection Facility SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID SHALE GAS; DEICING-SALTS; ROADSIDE SOILS; METAL MOBILITY; HEAVY-METALS; PENNSYLVANIA; EXTRACTION; IMPACTS; WELLS; PRECIPITATION AB The development of unconventional oil and gas (UOG) resources has rapidly increased in recent years; however, the environmental impacts and risks are poorly understood. A single well can generate millions of liters of wastewater, representing a mixture of formation brine and injected hydraulic fracturing fluids. One of the most common methods for wastewater disposal is underground injection; we are assessing potential risks of this method through an intensive, interdisciplinary study at an injection disposal facility in West Virginia. In June 2014, waters collected downstream from the site had elevated specific conductance (416 mu S/cm) and Na, Cl, Ba, Br, Sr, and Li concentrations, compared to upstream, background waters (conductivity, 74 mu S/cm). Elevated TDS, a marker of UOG wastewater, provided an early indication of impacts in the stream. Wastewater inputs are also evident by changes in Sr-87/Sr-86 in streamwater adjacent to the disposal facility. Sediments downstream from the facility were enriched in Ra and had high bioavailable Fe(III) concentrations relative to upstream sediments. Microbial communities in downstream sediments had lower diversity and shifts in composition. Although the hydrologic pathways were not able to be assessed, these data provide evidence demonstrating that activities at the disposal facility are impacting a nearby stream and altering the biogeochemistry of nearby ecosystems. C1 [Akob, Denise M.; Mumford, Adam C.; Klinges, J. Grace; Cozzarelli, Isabelle M.] US Geol Survey, Natl Res Program, Reston, VA 20192 USA. [Orem, William; Engle, Mark A.] US Geol Survey, Eastern Energy Resources Sci Ctr, Reston, VA 20192 USA. [Kent, Douglas B.] US Geol Survey, Natl Res Program, 345 Middlefield Rd, Menlo Pk, CA 94025 USA. [Klinges, J. Grace] US EPA, Off Superfund Remediat & Technol Innovat, Sci Policy Branch, Washington, DC USA. RP Akob, DM (reprint author), US Geol Survey, Natl Res Program, Reston, VA 20192 USA. EM dakob@usgs.gov OI Engle, Mark/0000-0001-5258-7374; Cozzarelli, Isabelle/0000-0002-5123-1007 FU USGS Toxic Substances Hydrology Program; USGS Hydrologic Research and Development Program; USGS Energy Resources Program FX This project was supported by the USGS Toxic Substances Hydrology Program, USGS Hydrologic Research and Development Program, and USGS Energy Resources Program. Appreciation is extended to Jeanne B. Jaeschke of the USGS for her assistance with fieldwork and laboratory analysis of water chemistry. The authors would also like to thank Kalla Fleger for field assistance, Chris Fuller for uranium and radium determinations, Greg Noe and Jaimie Gillespie for sediment elemental analysis, and Michelle Hladik for analysis of disinfection byproducts. We would like to thank Charles A. Cravotta III for helpful discussions. In addition, we would like to thank the site owner for facilitating research through site access to the US Geological Survey. NR 63 TC 5 Z9 5 U1 16 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 7 PY 2016 VL 50 IS 11 BP 5517 EP 5525 DI 10.1021/acs.est.6b00428 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DO2SI UT WOS:000377629900014 PM 27158829 ER PT J AU Leonard, JA Leonard, AS Chang, DT Edwards, S Lu, JT Scholle, S Key, P Winter, M Isaacs, K Tan, YM AF Leonard, Jeremy A. Leonard, Ashley Sobel Chang, Daniel T. Edwards, Stephen Lu, Jingtao Scholle, Steven Key, Phillip Winter, Maxwell Isaacs, Kristin Tan, Yu-Mei TI Evaluating the Impact of Uncertainties in Clearance and Exposure When Prioritizing Chemicals Screened in High-Throughput Assays SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID RISK-ASSESSMENT; IN-VITRO; CYTOCHROME-P450 ISOFORMS; TOTAL DIET; TOXICITY; PESTICIDES; MALATHION; METABOLISM; TOXICOLOGY; RAT AB The toxicity-testing paradigm has evolved to include high throughput (HT) methods for addressing the increasing need to screen hundreds to thousands of chemicals rapidly. Approaches that involve in vitro screening assays, in silico predictions of exposure concentrations, and pharmacokinetic (PK) characteristics provide the foundation for HT risk prioritization. Underlying uncertainties in predicted exposure concentrations or PR behaviors can significantly influence the prioritization of chemicals, though the impact of such influences is unclear. In the current study, a framework was developed to incorporate absorbed doses, PR properties, and in vitro dose response data into a PK/pharmacodynamic (PD) model to allow for placement of chemicals into discrete priority bins. Literature-reported or predicted values for clearance rates and absorbed doses were used in the PK/PD model to evaluate the impact of their uncertainties on chemical prioritization. Scenarios using predicted absorbed doses resulted in a larger number of bin misassignments than those scenarios using predicted clearance rates, when comparing to bin placement using literature-reported values. Sensitivity of parameters on the model output of toxicological activity was examined across possible ranges for those parameters to provide insight into how uncertainty in their predicted values might impact uncertainty in activity. C1 [Leonard, Jeremy A.; Lu, Jingtao] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Leonard, Ashley Sobel] Duke Univ, Dept Biol Sci, Durham, NC 27708 USA. [Chang, Daniel T.] Chem Comp Grp, Montreal, PQ H3A 2R7, Canada. [Edwards, Stephen] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27709 USA. [Scholle, Steven; Key, Phillip; Winter, Maxwell; Isaacs, Kristin; Tan, Yu-Mei] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27709 USA. RP Tan, YM (reprint author), US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27709 USA. EM tan.cecilia@epa.gov FU Oak Ridge Institute for Science and Education Research Participation Program at the U.S. EPA FX We thank Drs. Anna Lowit and Brandall Ingle for their review and comments. Drs. Jeremy Leonard and Jingtao Lu were funded through the Oak Ridge Institute for Science and Education Research Participation Program at the U.S. EPA. NR 79 TC 0 Z9 0 U1 3 U2 6 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 7 PY 2016 VL 50 IS 11 BP 5961 EP 5971 DI 10.1021/acs.est.6b00374 PG 11 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DO2SI UT WOS:000377629900065 PM 27124219 ER PT J AU Zhao, D Li, J Li, C Juhasz, AL Scheckel, KG Luo, J Li, HB Ma, LQ AF Zhao, Di Li, Jie Li, Chao Juhasz, Albert L. Scheckel, Kirk G. Luo, Jun Li, Hong-Bo Ma, Lena Q. TI Lead Relative Bioavailability in Lip Products and Their Potential Health Risk to Women SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID MICROWAVE-ASSISTED DIGESTION; MASS SPECTROMETRIC METHOD; CONTAMINATED SOILS; COSMETIC PRODUCTS; EXPOSURE; LIPSTICK; BIOACCESSIBILITY; PREGNANCY; METALS; MOBILIZATION AB Recent studies have investigated lead (Pb) concentrations in lip products but little is known about its oral bioavailability. In this study, 75 lipsticks and 18 lip glosses were assessed for Pb concentration, while 15 samples were assessed for Pb relative bioavailability (RBA, relative to Pb acetate absorption) using a mouse femur assay. Lead concentrations were 0.2-10 185 mg kg(-1), with 21 samples exceeding the Chinese limit of 40 mg kg(-1). Samples with orange and pink colors and/or low cost contained higher Pb concentrations. For samples with Pb > 7500 mg kg(-1), Pb was present due to the addition of lead chromate (PbCrO4) as a colorant, which was confirmed by X-ray absorption near-edge structure analysis. Lead RBA in 15 samples (87-10 185 mg kg(-1)) ranged from 23% to 95%, being significantly higher in moderate Pb (56-95%; 87-300 mg kg(-1)) than high Pb samples (23-48%; >300 mg kg(-1)). The calculation of Pb intake based on Pb-RBA showed that lip product ingestion contributed 5.4-68% of the aggregate Pb exposure for women depending on Pb concentration. The high Pb concentration in some lip products together with their moderate Pb-RBA suggests that lip product ingestion is a potential health concern to women. C1 [Zhao, Di; Li, Jie; Li, Chao; Luo, Jun; Li, Hong-Bo; Ma, Lena Q.] Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210046, Jiangsu, Peoples R China. [Juhasz, Albert L.] Univ S Australia, Future Ind Inst, Adelaide, SA 5095, Australia. [Scheckel, Kirk G.] US EPA, Natl Risk Management Res Lab, Land Remediat & Pollut Control Div, Cincinnati, OH 45224 USA. [Ma, Lena Q.] Univ Florida, Dept Soil & Water Sci, Gainesville, FL 32611 USA. RP Li, HB; Ma, LQ (reprint author), Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210046, Jiangsu, Peoples R China. EM hongboli@nju.edu.cn; lqma@ufl.edu RI Li, Hong-bo/A-1199-2015; Ma, Lena/E-5854-2010; Luo, Jun/I-2293-2012; OI Li, Hong-bo/0000-0003-1498-4285; Ma, Lena/0000-0002-8463-9957; Scheckel, Kirk/0000-0001-9326-9241 FU Jiangsu Provincial Innovation Team Program; Jiangsu Provincial Double Innovation Program FX This work was supported in part by Jiangsu Provincial Innovation Team Program and Jiangsu Provincial Double Innovation Program. NR 41 TC 1 Z9 1 U1 20 U2 44 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 7 PY 2016 VL 50 IS 11 BP 6036 EP 6043 DI 10.1021/acs.est.6b01425 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DO2SI UT WOS:000377629900073 PM 27187630 ER PT J AU Alman, BL Pfister, G Hao, H Stowell, J Hu, XF Liu, Y Strickland, MJ AF Alman, Breanna L. Pfister, Gabriele Hao, Hua Stowell, Jennifer Hu, Xuefei Liu, Yang Strickland, Matthew J. TI The association of wildfire smoke with respiratory and cardiovascular emergency department visits in Colorado in 2012: a case crossover study SO ENVIRONMENTAL HEALTH LA English DT Article DE Wildfires; Respiratory; Cardiovascular; Emergency department visits; PM2.5; Particulate matter ID FINE PARTICULATE MATTER; CARDIORESPIRATORY HOSPITAL ADMISSIONS; SOUTHERN CALIFORNIA WILDFIRES; OXIDATIVE STRESS; TIME-SERIES; FIRE SMOKE; HEALTH; AUSTRALIA; EMISSIONS; MODEL AB Background: In 2012, Colorado experienced one of its worst wildfire seasons of the past decade. The goal of this study was to investigate the relationship of local PM2.5 levels, modeled using the Weather Research and Forecasting Model with Chemistry, with emergency department visits and acute hospitalizations for respiratory and cardiovascular outcomes during the 2012 Colorado wildfires. Methods: Conditional logistic regression was used to assess the relationship between both continuous and categorical PM2.5 and emergency department visits during the wildfire period, from June 5th to July 6th 2012. Results: For respiratory outcomes, we observed positive relationships between lag 0 PM2.5 and asthma/wheeze (1 h max OR 1.01, 95 % CI (1.00, 1.01) per 10 mu g/m(3); 24 h mean OR 1.04 95 % CI (1.02, 1.06) per 5 mu g/m(3)), and COPD (1 h max OR 1.01 95 % CI (1.00, 1.02) per 10 mu g/m(3); 24 h mean OR 1.05 95 % CI (1.02, 1.08) per 5 mu g/m(3)). These associations were also positive for 2-day and 3-day moving average lag periods. When PM2.5 was modeled as a categorical variable, bronchitis also showed elevated effect estimates over the referent groups for lag 0 24 h average concentration. Cardiovascular results were consistent with no association. Conclusions: We observed positive associations between PM2.5 from wildfire and respiratory diseases, supporting evidence from previous research that wildfire PM2.5 is an important source for adverse respiratory health outcomes. C1 [Alman, Breanna L.] US EPA, Off Air Qual Planning & Stand, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Pfister, Gabriele] Natl Ctr Atmospher Res, 3450 Mitchell Lane, Boulder, CO 80301 USA. [Hao, Hua; Stowell, Jennifer; Hu, Xuefei; Liu, Yang] Emory Univ, Rollins Sch Publ Hlth, Dept Environm Hlth, 1518 Clifton Rd NE, Atlanta, GA 30322 USA. [Strickland, Matthew J.] Univ Nevada, Sch Community Hlth Sci, 1664 North Virginia St, Reno, NV 89557 USA. RP Alman, BL (reprint author), US EPA, Off Air Qual Planning & Stand, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM alman.breanna@epa.gov FU U.S. National Aeronautics and Space Administration (NASA) [NNX11AI53G]; NASA AQAST [NNX11AI51G] FX Funding from the U.S. National Aeronautics and Space Administration (NASA) grant NNX11AI53G and NASA AQAST project NNX11AI51G. The funding agency had no input on the design of the study, on the collection, analysis, and interpretation of data, or in the writing the manuscript. NCAR is operated by the University Corporation of Atmospheric Research under sponsorship of the National Science Foundation. NR 32 TC 1 Z9 1 U1 7 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1476-069X J9 ENVIRON HEALTH-GLOB JI Environ. Health PD JUN 4 PY 2016 VL 15 AR 64 DI 10.1186/s12940-016-0146-8 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DO0YX UT WOS:000377506200002 PM 27259511 ER PT J AU Ho, CK Christian, JM Ching, EJ Slavin, J Ortega, J Murray, R Rossman, LA AF Ho, Clifford K. Christian, Joshua M. Ching, Eric J. Slavin, Jason Ortega, Jesus Murray, Regan Rossman, Lewis A. TI Sediment Resuspension and Transport in Water Distribution Storage Tanks SO JOURNAL AMERICAN WATER WORKS ASSOCIATION LA English DT Article DE sediment resuspension; storage tank; water distribution; water quality ID COMPUTATIONAL FLUID-DYNAMICS; URBAN DRAINAGE; MOVEMENT; THRESHOLD; SYSTEMS; STRESS; FLOW AB Computational and experimental studies were conducted to better understand conditions that affect particle resuspension and movement in water storage tanks. Parameters that were investigated included inlet/outlet (I/O) line location and diameter, flow rate, particle size, and filling versus draining cycles. Simulation results showed that smaller particle sizes, higher flow rates, and draining cycles yielded the greatest potential for particle resuspension, which was generally limited to regions near the I/O line. Small-scale experiments were also performed using different sizes of glass beads and silica sand; the results generally validated the models. Mitigation methods were also presented to reduce the amount of particle resuspension. A pipe that extended from the I/O line into the tank (slightly above the bottom floor) was found to significantly reduce the potential for particle resuspension in both the computational models and experiments. C1 [Ho, Clifford K.; Christian, Joshua M.; Ching, Eric J.; Slavin, Jason; Ortega, Jesus] Sandia Natl Labs, POB 5800, Albuquerque, NM 87185 USA. [Murray, Regan; Rossman, Lewis A.] US EPA, Cincinnati, OH 45268 USA. RP Ho, CK (reprint author), Sandia Natl Labs, POB 5800, Albuquerque, NM 87185 USA. EM ckho@sandia.gov FU US Department of Energy's National Nuclear Security Administration [DE-AC04-94AL85000]; USEPA through its Office of Research and Development FX The authors acknowledge the helpful information provided by the Albuquerque Water Authority (German Andrade), Greater Cincinnati Water Works (Yeongho Lee), and Bohannan Huston Inc. (Todd Burt). Sandia National Laboratories is a multiprogram laboratory managed and operated by Sandia Corp., a wholly owned subsidiary of Lockheed Martin Corp., for the US Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. The USEPA, through its Office of Research and Development, funded and collaborated on the research described here. The research has been subjected to the USEPA's review and approved for publication. Note that approval does not signify that the contents necessarily reflect the views of USEPA. Mention of trade names, products, or services does not convey official USEPA approval, endorsement, or recommendation. NR 36 TC 0 Z9 0 U1 2 U2 2 PU AMER WATER WORKS ASSOC PI DENVER PA 6666 W QUINCY AVE, DENVER, CO 80235 USA SN 2164-4535 J9 J AM WATER WORKS ASS JI J. Am. Water Work Assoc. PD JUN PY 2016 VL 108 IS 6 BP E349 EP E361 DI 10.5942/jawwa.2016.108.0077 PG 13 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA EL3HI UT WOS:000394509800005 ER PT J AU Mix, N Thompson, KA AF Mix, Nelson Thompson, Kenneth A. TI Improving Water System Resiliency and Security: Advanced Metering Infrastructure SO JOURNAL AMERICAN WATER WORKS ASSOCIATION LA English DT Article DE metering; response; security; systems AB This article documents lessons learned from US Environmental Protection Agency (USEPA) interviews with representatives of eight leading US water utilities that have implemented advanced metering infrastructure (AMI) to improve the resiliency and security of their water distribution systems. Improvements were made using noncustomer billing functions to detect customer leaks, reverse flow, and meter tampering. Insights that can be leveraged by other water utilities as well as gaps that reduce the benefits of the new functions are identified and discussed. In addition, USEPA promotes the use of a surveillance and response system (SRS) to improve the detection and response time to water quality incidents within the distribution system. An SRS integrates information from multiple monitoring and surveillance components to alert the utility to water quality anomalies, which could be associated with possible contamination. On the basis of positive anecdotes from the interviews, application of AMI as a component of an SRS is also reviewed. C1 [Mix, Nelson] US EPA, US Publ Hlth Serv, 1200 Penn Ave NW,MC4608T, Washington, DC 20460 USA. [Thompson, Kenneth A.] CH2M, Intelligent Water Solut, Englewood, CO USA. RP Mix, N (reprint author), US EPA, US Publ Hlth Serv, 1200 Penn Ave NW,MC4608T, Washington, DC 20460 USA. EM mix.nelson@epa.gov NR 10 TC 0 Z9 0 U1 0 U2 0 PU AMER WATER WORKS ASSOC PI DENVER PA 6666 W QUINCY AVE, DENVER, CO 80235 USA SN 2164-4535 J9 J AM WATER WORKS ASS JI J. Am. Water Work Assoc. PD JUN PY 2016 VL 108 IS 6 BP E310 EP E317 DI 10.5942/jawwa.2016.108.0048 PG 8 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA EL3HI UT WOS:000394509800001 ER PT J AU Lane, CR D'Amico, E AF Lane, Charles R. D'Amico, Ellen TI IDENTIFICATION OF PUTATIVE GEOGRAPHICALLY ISOLATED WETLANDS OF THE CONTERMINOUS UNITED STATES SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE connectivity; data management; geospatial analysis; palustrine wetlands; rivers/streams/lakes; watershed management ID OBJECT-ORIENTED CLASSIFICATION; PRAIRIE POTHOLE REGION; DEPRESSIONAL WETLANDS; ECOSYSTEM SERVICES; PLAYA WETLANDS; VERNAL POOLS; GULF-COAST; LANDSCAPE; CONNECTIVITY; HYDROLOGY AB Geographically isolated wetlands (GIWs) are wetlands completely surrounded by uplands. While common throughout the United States (U.S.), there have heretofore been no nationally available, spatially explicit estimates of GIW extent, complicating efforts to understand the myriad biogeochemical, hydrological, and habitat functions of GIWs and hampering conservation and management efforts at local, state, and national scales. We used a 10-m geospatial buffer as a proxy for hydrological or ecological connectivity of National Wetlands Inventory palustrine and lacustrine wetland systems to nationally mapped and available stream, river, and lake data. We identified over 8.3 million putative GIWs across the conterminous U.S., encompassing nearly 6.5 million hectares of wetland resources (average size 0.79 +/- 4.81 ha, median size 0.19 ha). Putative GIWs thus represent approximately 16% of the freshwater wetlands of the conterminous U.S. The water regime for the majority of the putative GIWs was temporarily or seasonally flooded, suggesting a vulnerability to ditching or hydrologic abstraction, sedimentation, or alterations in precipitation patterns. Additional analytical applications of this readily available geospatially explicit mapping product (e.g., hydrological modeling, amphibian metapopulation, or landscape ecological analyses) will improve our understanding of the abundance and extent, effect, connectivity, and relative importance of GIWs to other aquatic systems of the conterminous U.S. C1 [Lane, Charles R.] US EPA, Off Res & Dev, 26 W Martin Luther King Dr,MS 581, Cincinnati, OH 45268 USA. [D'Amico, Ellen] CSS Dynamac Corp, Cincinnati, OH 45268 USA. RP Lane, CR (reprint author), US EPA, Off Res & Dev, 26 W Martin Luther King Dr,MS 581, Cincinnati, OH 45268 USA. EM lane.charle-s@epa.gov NR 97 TC 1 Z9 1 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2016 VL 52 IS 3 BP 705 EP 722 DI 10.1111/1752-1688.12421 PG 18 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DY3PW UT WOS:000385007100010 ER PT J AU Simpson, RD AF Simpson, R. David TI Putting a Price on Ecosystem Services SO ISSUES IN SCIENCE AND TECHNOLOGY LA English DT Article ID CONSERVATION C1 [Simpson, R. David] US EPA, Natl Ctr Environm Econ, Ecosyst Econ Studies, Washington, DC 20460 USA. RP Simpson, RD (reprint author), US EPA, Natl Ctr Environm Econ, Ecosyst Econ Studies, Washington, DC 20460 USA. NR 5 TC 0 Z9 0 U1 1 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0748-5492 EI 1938-1557 J9 ISSUES SCI TECHNOL JI Issues Sci. Technol. PD SUM PY 2016 VL 32 IS 4 BP 57 EP 62 PG 6 WC Engineering, Multidisciplinary; Engineering, Industrial; Multidisciplinary Sciences; Social Issues SC Engineering; Science & Technology - Other Topics; Social Issues GA DX9IN UT WOS:000384706900024 ER PT J AU Wobus, C Flanner, M Sarofim, MC Moura, MCP Smith, SJ AF Wobus, Cameron Flanner, Mark Sarofim, Marcus C. Moura, Maria Cecilia P. Smith, Steven J. TI Future Arctic temperature change resulting from a range of aerosol emissions scenarios SO EARTHS FUTURE LA English DT Article ID CLIMATE-CHANGE; BLACK CARBON; FORCERS; AMPLIFICATION; STABILIZATION; MITIGATION; PATHWAY AB The Arctic temperature response to emissions of aerosols-specifically black carbon (BC), organic carbon (OC), and sulfate-depends on both the sector and the region where these emissions originate. Thus, the net Arctic temperature response to global aerosol emissions reductions will depend strongly on the blend of emissions sources being targeted. We use recently published equilibrium Arctic temperature response factors for BC, OC, and sulfate to estimate the range of present-day and future Arctic temperature changes from seven different aerosol emissions scenarios. Globally, Arctic temperature changes calculated from all of these emissions scenarios indicate that present-day emissions from the domestic and transportation sectors generate the majority of present-day Arctic warming from BC. However, in all of these scenarios, this warming is more than offset by cooling resulting from SO2 emissions from the energy sector. Thus, long-term climate mitigation strategies that are focused on reducing carbon dioxide (CO2) emissions from the energy sector could generate short-term, aerosol-induced Arctic warming. A properly phased approach that targets BC-rich emissions from the transportation sector as well as the domestic sectors in key regions-while simultaneously working toward longer-term goals of CO2 mitigation-could potentially avoid some amount of short-term Arctic warming. C1 [Wobus, Cameron] Abt Associates Inc, Boulder, CO 80302 USA. [Flanner, Mark] Univ Michigan, Dept Climate & Space Sci & Engn, Ann Arbor, MI 48109 USA. [Sarofim, Marcus C.] US EPA, Washington, DC 20460 USA. [Moura, Maria Cecilia P.; Smith, Steven J.] Pacific Northwest Natl Lab, Joint Global Change Res Inst, College Pk, MD USA. RP Wobus, C (reprint author), Abt Associates Inc, Boulder, CO 80302 USA. EM cameron_wobus@abtassoc.com RI Flanner, Mark/C-6139-2011 OI Flanner, Mark/0000-0003-4012-174X FU U.S. Environmental Protection Agency (EPA) FX We thank Maria Sand for providing data on Arctic temperature response, and Joseph Donahue for project support. Funding for this work was provided through a U.S. Environmental Protection Agency (EPA) contract to Abt Associates (formerly Stratus Consulting). All of the data used in this analysis are publicly available from the repositories cited in the manuscript text. The views expressed in this paper are those of the authors and do not necessarily reflect those of the EPA. NR 29 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2328-4277 J9 EARTHS FUTURE JI Earth Future PD JUN PY 2016 VL 4 IS 6 BP 270 EP 281 DI 10.1002/2016EF000361 PG 12 WC Environmental Sciences; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Geology; Meteorology & Atmospheric Sciences GA DV1LM UT WOS:000382682600001 ER PT J AU Hughes, MF Ross, DG Starr, JM Scollon, EJ Wolansky, MJ Crofton, KM DeVito, MJ AF Hughes, Michael F. Ross, David G. Starr, James M. Scollon, Edward J. Wolansky, Marcelo J. Crofton, Kevin M. DeVito, Michael J. TI Environmentally relevant pyrethroid mixtures: A study on the correlation of blood and brain concentrations of a mixture of pyrethroid insecticides to motor activity in the rat SO TOXICOLOGY LA English DT Article DE Pesticide; Pyrethroid; Neurotoxicity; Mixture; Dosimetry ID IN-VITRO METABOLISM; ACOUSTIC STARTLE RESPONSE; CYTOCHROME-P450 ISOFORMS; HYDROLYTIC METABOLISM; SUBSTRATE-SPECIFICITY; DELTAMETHRIN; LIVER; TOXICITY; CARBOXYLESTERASES; PESTICIDES AB Human exposure to multiple pyrethroid insecticides may occur because of their broad use on crops and for residential pest control. To address the potential health risk from co-exposure to pyrethroids, it is important to understand their disposition and toxicity in target organs such as the brain, and surrogates such as the blood when administered as a mixture. The objective of this study was to assess the correlation between blood and brain concentrations of pyrethroids and neurobehavioral effects in the rat following an acute oral administration of the pyrethroids as a mixture. Male Long-Evans rats were administered a mixture of P-cyfluthrin, cypermethrin, deltamethrin, esfenvalerate and cis- and transpermethrin in corn oil at seven dose levels. The pyrethroid with the highest percentage in the dosing solution was trans-permethrin (31% of total mixture dose) while deltamethrin and esfenvalerate had the lowest percentage (3%). Motor activity of the rats was then monitored for 1 h. At 3.5 h post-dosing, the animals were euthanized and blood and brain were collected. These tissues were extracted and analyzed for parent pyrethroid using HPLC-tandem mass spectrometry. Cypermethrin and cis-permethrin were the predominate pyrethroids detected in blood and brain, respectively, at all dosage levels. The relationship of total pyrethroid concentration between blood and brain was linear (r = 0.93). The pyrethroids with the lowest fraction in blood were trans-permethrin and beta-cyfluthrin and in brain were deltamethrin and esfenvalerate. The relationship between motor activity of the treated rats and summed pyrethroid blood and brain concentration was described using a sigmoidal E-max model with the Effective Concentrations being more sensitive for brain than blood. The data suggests summed pyrethroid rat blood concentration could be used as a surrogate for brain concentration as an aid to study the neurotoxic effects of pyrethroids administered as a mixture under the conditions used in this study. Published by Elsevier Ireland Ltd. C1 [Hughes, Michael F.; Ross, David G.; Scollon, Edward J.; Crofton, Kevin M.; DeVito, Michael J.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, MD B105-03, Res Triangle Pk, NC 27709 USA. [Starr, James M.] US EPA, Off Res & Dev, Natl Exposure Res Lab, MD B105-03, Res Triangle Pk, NC 27709 USA. [Wolansky, Marcelo J.] CNR, Res Triangle Pk, NC USA. [Scollon, Edward J.] Syngenta Crop Protect, Greensboro, NC USA. [Wolansky, Marcelo J.] Univ Buenos Aires, Buenos Aires, DF, Argentina. [Wolansky, Marcelo J.] Argentine NRC Inst IQUIBICEN, Buenos Aires, DF, Argentina. [Crofton, Kevin M.] US EPA, Off Res & Dev, Natl Ctr Computat Toxicol, MD B105-03, Res Triangle Pk, NC 27709 USA. [DeVito, Michael J.] NIEHS, Natl Toxicol Program, POB 12233, Res Triangle Pk, NC 27709 USA. RP Hughes, MF (reprint author), US EPA, MD B105-03, Res Triangle Pk, NC 27709 USA. EM hughes.michaelf@epa.gov FU internal US Environmental Protection Agency, Office of Research and Development funds FX The authors thank FMC Corporation, Bayer CropScience and DuPont Crop Protection for providing the pyrethroids used in this study. The authors also thank Drs. Hisham El-Masri, Jane Ellen Simmons and Nicole Tulve for their review of an earlier version of this manuscript. This research was supported by internal US Environmental Protection Agency, Office of Research and Development funds. NR 44 TC 2 Z9 2 U1 8 U2 8 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PD JUN 1 PY 2016 VL 359 BP 19 EP 28 DI 10.1016/j.tox.2016.06.013 PG 10 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA DT5TM UT WOS:000381545500003 PM 27330022 ER PT J AU Olden, K Lin, YS Bussard, D AF Olden, Kenneth Lin, Yu-Sheng Bussard, David TI Epigenome: A Biomarker or Screening Tool to Evaluate Health Impact of Cumulative Exposure to Chemical and Non-Chemical Stressors SO BIOSENSORS-BASEL LA English DT Article DE chemical; cumulative; DNA methylation; environmental; epigenetics; epigenome; non-chemical; histone modification; stressors ID DIFFERENTIAL DNA METHYLATION; ENVIRONMENTAL EXPOSURES; TOBACCO-SMOKE; IN-UTERO; EPIGENETICS; CANCER; RISK; DISCOVERY; DISEASES; ISLANDS AB Current risk assessment practices and toxicity information are hard to utilize for assessing the health impact of combined or cumulative exposure to multiple chemical and non-chemical stressors encountered in the "real world" environment. Non-chemical stressors such as heat, radiation, noise, humidity, bacterial and viral agents, and social factors, like stress related to violence and socioeconomic position generally cannot be currently incorporated into the risk assessment paradigm. The Science and Decisions report released by the National Research Council (NRC) in 2009 emphasized the need to characterize the effects of multiple stressors, both chemical and non-chemical exposures. One impediment to developing information relating such non-chemical stressors to health effects and incorporating them into cumulative assessment has been the lack of analytical tools to easily and quantitatively monitor the cumulative exposure to combined effects of stressors over the life course. C1 [Olden, Kenneth; Lin, Yu-Sheng; Bussard, David] US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Washington, DC 20460 USA. RP Olden, K (reprint author), US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Washington, DC 20460 USA. EM Olden.kenneth@Epa.gov; Lin.Yu-Sheng@epa.gov; bussard.david@epa.gov OI Lin, Yu-Sheng/0000-0002-6813-7826 NR 52 TC 0 Z9 0 U1 1 U2 1 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2079-6374 J9 BIOSENSORS-BASEL JI Biosensors-Basel PD JUN PY 2016 VL 6 IS 2 AR 12 DI 10.3390/bios6020012 PG 7 WC Chemistry, Analytical SC Chemistry GA DT9YR UT WOS:000381858800002 PM 27398233 ER PT J AU Martinez, JC Caprio, MA AF Martinez, J. C. Caprio, M. A. TI IPM Use With the Deployment of a Non-High Dose Bt Pyramid and Mitigation of Resistance for Western Corn Rootworm (Diabrotica virgifera virgifera) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Bt pyramid; hotspot resistance; mitigation; western corn rootworm; IPM ID FIELD-EVOLVED RESISTANCE; COLEOPTERA-CHRYSOMELIDAE; TRANSGENIC CORN; SOIL INSECTICIDES; DISPERSAL KERNELS; FITNESS COSTS; SEED MIXTURES; ADAPTATION; MAIZE; MANAGEMENT AB Recent detection of western corn rootworm resistance to Bt (Bacillus thuringiensis) corn prompted recommendations for the use of integrated pest management (IPM) with planting refuges to prolong the durability of Bt technologies. We conducted a simulation experiment exploring the effectiveness of various IPM tools at extending durability of pyramided Bt traits. Results indicate that some IPM practices have greater merits than others. Crop rotation was the most effective strategy, followed by increasing the non-'Bt refuge size from 5 to 20%. Soil-applied insecticide use for Bt corn did not increase the durability compared with planting Bt with refuges alone, and both projected lower durabilities. When IPM participation with randomly selected management tools was increased at the time of Bt commercialization, durability of pyramided traits increased as well. When non-corn rootworm expressing corn was incorporated as an IPM option, the durability further increased. For corn rootworm, a local resistance phenomenon appeared immediately surrounding the resistant field (hotspot) and spread throughout the local neighborhood in six generations in absence of mitigation. Hotspot mitigation with random selection of strategies was ineffective at slowing resistance, unless crop rotation occurred immediately; regional mitigation was superior to random mitigation in the hotspot and reduced observed resistance allele frequencies in the neighborhood. As resistance alleles of mobile pests can escape hotspots, the scope of mitigation should extend beyond resistant sites. In the case of widespread resistance, regional mitigation was less effective at prolonging the life of the pyramid than IPM with Bt deployment at the time of commercialization. C1 [Martinez, J. C.] US EPA, Off Pesticide Programs, 1200 Pennsylvania Ave,NW,Mail Code 7511-P, Washington, DC 20460 USA. [Caprio, M. A.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. RP Martinez, JC (reprint author), US EPA, Off Pesticide Programs, 1200 Pennsylvania Ave,NW,Mail Code 7511-P, Washington, DC 20460 USA. EM Martinez.Jeannette@epa.gov; mcaprio@entomology.msstate.edu NR 79 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 747 EP 761 DI 10.1093/ee/nvw015 PG 15 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800026 ER PT J AU Eason, T Garmestani, AS Stow, CA Rojo, C Alvarez-Cobelas, M Cabezas, H AF Eason, Tarsha Garmestani, Ahjond S. Stow, Craig A. Rojo, Carmen Alvarez-Cobelas, Miguel Cabezas, Heriberto TI Managing for resilience: an information theory-based approach to assessing ecosystems SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE ecosystems; Fisher information; indicators; indices; information theory; leading indicators; multivariate; regime shifts; resilience ID ECOLOGICAL REGIME SHIFTS; EARLY-WARNING SIGNALS; FISHER INFORMATION; CRITICAL TRANSITIONS; SYSTEMS; SUSTAINABILITY; INDICATORS; PACIFIC; DROUGHT; TIME AB 1. Ecosystems are complex and multivariate; hence, methods to assess the dynamics of ecosystems should have the capacity to evaluate multiple indicators simultaneously. 2. Most research on identifying leading indicators of regime shifts has focused on univariate methods and simple models which have limited utility when evaluating real ecosystems, particularly because drivers are often unknown. 3. We discuss some common univariate and multivariate approaches for detecting critical transitions in ecosystems and demonstrate their capabilities via case studies. 4. Synthesis and applications. We illustrate the utility of an information theory-based index for assessing ecosystem dynamics. Trends in this index also provide a sentinel of both abrupt and gradual transitions in ecosystems. C1 [Eason, Tarsha; Garmestani, Ahjond S.; Cabezas, Heriberto] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Stow, Craig A.] NOAA, Great Lakes Environm Res Lab, Ann Arbor, MI 48108 USA. [Rojo, Carmen] Univ Valencia, Cavanilles Inst Biodivers & Evolutionary Biol, Valencia, Spain. [Alvarez-Cobelas, Miguel] Spanish Natl Res Council, Natl Museum Nat Hist, Madrid, Spain. [Cabezas, Heriberto] Univ Pannonia, Dept Comp Sci & Syst Technol, H-8200 Veszprem, Hungary. RP Eason, T (reprint author), US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. EM eason.tarsha@epa.gov FU United States Geological Survey's John Wesley Powell Center for Analysis and Synthesis FX This paper is an outwork of discussions at the Managing for Resilience Working Group, funded by the United States Geological Survey's John Wesley Powell Center for Analysis and Synthesis. We thank two anonymous reviewers for their helpful comments. The views expressed in this paper are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. GLERL contribution number 1786. NR 61 TC 4 Z9 4 U1 17 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD JUN PY 2016 VL 53 IS 3 BP 656 EP 665 DI 10.1111/1365-2664.12597 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR7FM UT WOS:000380065400005 ER PT J AU Angeler, DG Allen, CR Barichievy, C Eason, T Garmestani, AS Graham, NAJ Granholm, D Gunderson, LH Knutson, M Nash, KL Nelson, RJ Nystrom, M Spanbauer, TL Stow, CA Sundstrom, SM AF Angeler, David G. Allen, Craig R. Barichievy, Chris Eason, Tarsha Garmestani, Ahjond S. Graham, Nicholas A. J. Granholm, Dean Gunderson, Lance H. Knutson, Melinda Nash, Kirsty L. Nelson, R. John Nystrom, Magnus Spanbauer, Trisha L. Stow, Craig A. Sundstrom, Shana M. TI Management applications of discontinuity theory SO JOURNAL OF APPLIED ECOLOGY LA English DT Review DE biodiversity; discontinuity theory; ecological complexity; extinction; invasion biology; management; monitoring; regime shifts; resilience; scale ID HIERARCHICAL PATCH DYNAMICS; ECOLOGICAL RESILIENCE; RELATIVE RESILIENCE; FUNCTIONAL-GROUPS; COMPLEX-SYSTEMS; REGIME SHIFTS; ENVIRONMENTAL-CHANGE; RESPONSE DIVERSITY; CLIMATE-CHANGE; CORAL-REEFS AB 1. Human impacts on the environment are multifaceted and can occur across distinct spatiotemporal scales. Ecological responses to environmental change are therefore difficult to predict, and entail large degrees of uncertainty. Such uncertainty requires robust tools for management to sustain ecosystem goods and services and maintain resilient ecosystems. 2. We propose an approach based on discontinuity theory that accounts for patterns and processes at distinct spatial and temporal scales, an inherent property of ecological systems. Discontinuity theory has not been applied in natural resource management and could therefore improve ecosystem management because it explicitly accounts for ecological complexity. 3. Synthesis and applications. We highlight the application of discontinuity approaches for meeting management goals. Specifically, discontinuity approaches have significant potential to measure and thus understand the resilience of ecosystems, to objectively identify critical scales of space and time in ecological systems at which human impact might be most severe, to provide warning indicators of regime change, to help predict and understand biological invasions and extinctions and to focus monitoring efforts. Discontinuity theory can complement current approaches, providing a broader paradigm for ecological management and conservation. C1 [Angeler, David G.] Swedish Univ Agr Sci, Dept Aquat Sci & Assessment, Box 7050, SE-75007 Uppsala, Sweden. [Allen, Craig R.] Univ Nebraska, US Geol Survey, Nebraska Cooperat Fish & Wildlife Res Unit, Lincoln, NE 68583 USA. [Barichievy, Chris] Zool Soc London, Regents Pk, London NW1 4RY, England. [Eason, Tarsha; Garmestani, Ahjond S.] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Graham, Nicholas A. J.; Nash, Kirsty L.] James Cook Univ, ARC Ctr Excellence Coral Reef Studies, Townsville, Qld 4811, Australia. [Granholm, Dean] US Fish & Wildlife Serv, Bloomington, MN 55437 USA. [Gunderson, Lance H.] Emory Univ, Dept Environm Sci, Atlanta, GA 30322 USA. [Knutson, Melinda] US Fish & Wildlife Serv, La Crosse, WI 54603 USA. [Nelson, R. John] Univ Victoria, Dept Biol, Ctr Biomed Res, Victoria, BC V8P 5C2, Canada. [Nelson, R. John] Stantec Consulting Ltd, Saanichton, BC V8M 2A5, Canada. [Nystrom, Magnus] Stockholm Univ, Stockholm Resilience Ctr, SE-10691 Stockholm, Sweden. [Spanbauer, Trisha L.] Univ Nebraska, Dept Earth & Atmospher Sci, Lincoln, NE 68583 USA. [Spanbauer, Trisha L.; Sundstrom, Shana M.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Stow, Craig A.] NOAA, Great Lakes Environm Res Lab, Ann Arbor, MI 48108 USA. RP Angeler, DG (reprint author), Swedish Univ Agr Sci, Dept Aquat Sci & Assessment, Box 7050, SE-75007 Uppsala, Sweden. EM david.angeler@slu.se RI Graham, Nicholas/C-8360-2014; OI Sundstrom, Shana/0000-0003-0823-8008; Nash, Kirsty/0000-0003-0976-3197 FU U.S. Geological Survey; Nebraska Game and Parks Commission; University of Nebraska-Lincoln; United States Fish and Wildlife Service; Wildlife Management Institute; August T. Larsson Foundation of the Swedish University of Agricultural Sciences; U.S. Geological Survey John Wesley Powell Center for Analysis and Synthesis; Swedish Research Council Formas [2014-1193]; Swedish Research Council Vetenskapsradet [2014-5828] FX The Nebraska Cooperative Fish and Wildlife Research Unit is jointly supported by a cooperative agreement between the U.S. Geological Survey, the Nebraska Game and Parks Commission, the University of Nebraska-Lincoln, the United States Fish and Wildlife Service and the Wildlife Management Institute. The views expressed herein are those of the authors and do not necessarily represent those of the United States Government or U.S. EPA. We gratefully acknowledge funding from the August T. Larsson Foundation of the Swedish University of Agricultural Sciences, the U.S. Geological Survey John Wesley Powell Center for Analysis and Synthesis and the Swedish Research Councils Formas (2014-1193) and Vetenskapsradet (2014-5828). Joseph Bennett and two reviewers provided helpful comments that improved the paper. This paper is GLERL contribution number 1767. NR 75 TC 5 Z9 6 U1 21 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD JUN PY 2016 VL 53 IS 3 BP 688 EP 698 DI 10.1111/1365-2664.12494 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR7FM UT WOS:000380065400008 ER PT J AU Bennett, KM AF Bennett, Kathleen M. TI Research pathways and outreach to drive cellulosic nanomaterials development SO TAPPI JOURNAL LA English DT Article AB There are many opportunities for development and use of cellulosic nanomaterials, sustainably made from renewable raw materials. Significant challenges in manufacturing and application are being overcome. The Agenda 2020 Technology Alliance has issued a technology roadmap identifying key research challenges in a number of priority areas. The purpose of the roadmap is to seek the engagement of researchers and the support of partners to execute the identified research needs to accelerate commercialization. New research funding opportunities are identified. In addition to the research challenges, there are communications needs that must also be addressed, and public-private partnerships that must be executed. C1 [Bennett, Kathleen M.] Bowater, Georgia Pacific & Ft James, Montreal, PQ, Canada. [Bennett, Kathleen M.] US EPA, Reagan Adm, Washington, DC 20460 USA. [Bennett, Kathleen M.] TAPPI, Norcross, GA USA. [Bennett, Kathleen M.] Georgia Inst Technol, Renewable Bioprod Inst, Atlanta, GA 30332 USA. RP Bennett, KM (reprint author), Georgia Inst Technol, Renewable Bioprod Inst, Atlanta, GA 30332 USA. FU TAPPI FX Thanks to Ted Wegner of the Forest Products Laboratory, Kim Nelson of American Process Inc., Michael Goergen of the P3Nano Project, U.S. Endowment for Forestry and Communities, and Peter Hart of WestRock for contributing ideas to this article. A special thanks to TAPPI for its support in publicizing the availability of the Agenda 2020 research roadmaps to the communities of interest. NR 1 TC 0 Z9 0 U1 1 U2 1 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD JUN PY 2016 VL 15 IS 6 BP 358 EP 361 PG 4 WC Materials Science, Paper & Wood SC Materials Science GA DS0EJ UT WOS:000380267900002 ER PT J AU Bracey, AM Etterson, MA Niemi, GJ Green, RF AF Bracey, Annie M. Etterson, Matthew A. Niemi, Gerald J. Green, Richard F. TI VARIATION IN BIRD-WINDOW COLLISION MORTALITY AND SCAVENGING RATES WITHIN AN URBAN LANDSCAPE SO WILSON JOURNAL OF ORNITHOLOGY LA English DT Article DE avian mortality; detectability; Great Lakes; migration; multistate Markov model; scavenging; window kills ID GREAT-LAKES; MIGRATION; REMOVAL; CARCASS; RADAR; PERSISTENCE; INJURIES; CANADA; RISK AB Avian mortality from collisions with windows and buildings is one of the top sources of anthropogenic mortality of birds. Each year in the United States, an estimated 100 million to one billion birds die from window collisions. Many studies of bird-window collision mortality have aimed to identify architectural, landscape, and species-specific factors that may influence collision rates, but little research has assessed the potential for spatiotemporal variation in collision mortality. We studied window collision mortality at 42 residential houses located within an urban landscape, along the shores of Lake Superior in Duluth MN, USA from 2006-2009 to quantify window-related fatalities during migration. The rate of window collision mortality was modeled as a function of house location and season using Poisson regression. We also conducted carcass distribution trials to estimate scavenging and detection rates, and analyzed the resulting data using a multistate Markov model. We used hierarchical models of scavenging probability to compare the relationship between scavenging rates and six measured covariates. Models for collision mortality and scavenging were evaluated using Akaike's Information Criterion (AIC). The adjusted number of birds killed (N-k) over the full course of monitoring was estimated using the Horvitz-Thompson estimator. A total of 40 species and 108 individual birds were recorded as window kills. Fatalities increased with distance from the city center, were higher at houses on the lake side of the study site, and on windows facing Lake Superior. Scavenging rates also increased with distance from the city center, with small carcasses being removed more quickly than large carcasses, and removal rates decreasing over time for all carcass sizes. Because of the low detection probability of homeowners, combined detection by both homeowners and researchers was <20%. Although mortality and scavenging rates were not uniformly distributed, we estimated an adjusted mortality rate of similar to 11-16 birds per house during the study period. Mortality estimates for all residential houses on Minnesota Point (n = 520) during the study period (n = 211 days) was 5,819-8,382 birds, similar to 1,421 birds per season. Results suggest spatiotemporal variation in both mortality and scavenging rates within our study area, both of which increased with distance from the city center. Houses with highest collision mortality also had the highest scavenging rates. Our results are consistent with other studies that have observed heterogeneity in mortality and scavenging rates associated with local structural and landscape level variables. Documenting patterns associated with increased collision mortality will be important in identifying locations that may pose a greater risk to birds. C1 [Bracey, Annie M.; Niemi, Gerald J.] Nat Resources Res Inst, 5013 Miller Trunk Highway, Duluth, MN 55811 USA. [Bracey, Annie M.; Niemi, Gerald J.] Univ Minnesota, Dept Biol Sci, 1049 Univ Dr, Duluth, MN 55812 USA. [Etterson, Matthew A.] US EPA, Off Res & Dev, NHEERL, Midcontinent Ecol Div, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Green, Richard F.] Univ Minnesota, Dept Math & Stat, 1049 Univ Dr, Duluth, MN 55812 USA. RP Bracey, AM (reprint author), Nat Resources Res Inst, 5013 Miller Trunk Highway, Duluth, MN 55811 USA.; Bracey, AM (reprint author), Univ Minnesota, Dept Biol Sci, 1049 Univ Dr, Duluth, MN 55812 USA. EM brace005@d.umn.edu NR 48 TC 0 Z9 0 U1 29 U2 29 PU WILSON ORNITHOLOGICAL SOC PI WACO PA 5400 BOSQUE BLVD, STE 680, WACO, TX 76710 USA SN 1559-4491 EI 1938-5447 J9 WILSON J ORNITHOL JI Wilson J. Ornithol. PD JUN PY 2016 VL 128 IS 2 BP 355 EP 367 PG 13 WC Ornithology SC Zoology GA DQ8GN UT WOS:000379447200012 ER PT J AU McGinty, MD Burke, TA Barnett, DJ Smith, KC Resnick, B Rutkow, L AF McGinty, Meghan D. Burke, Thomas A. Barnett, Daniel J. Smith, Katherine C. Resnick, Beth Rutkow, Lainie TI Hospital Evacuation and Shelter-in-Place: Who Is Responsible for Decision-Making? SO DISASTER MEDICINE AND PUBLIC HEALTH PREPAREDNESS LA English DT Article DE hospital evacuation; hospital shelter-in-place; organizational decision-making; emergency preparedness; disasters AB Objective During natural disasters, hospital evacuation may be necessary to ensure patient safety and care. We aimed to examine perceptions of stakeholders involved in these decisions throughout the Mid-Atlantic region of the United States during Hurricane Sandy in October 2012. Methods Semistructured interviews were conducted from March 2014 to February 2015 to characterize stakeholders' perceptions about authority and responsibility for acute care hospital evacuation/shelter-in-place decision-making in Delaware, Maryland, New Jersey, and New York during Hurricane Sandy. Interviews were recorded, transcribed, and thematically analyzed using a framework approach. Results We interviewed 42 individuals from 32 organizations. Hospital executives from all states reported having authority and responsibility for evacuation/shelter-in-place decision-making. In New York and Maryland, government officials stated that they could order hospital evacuation, whereas officials in Delaware and New Jersey said the government lacked enforcement capacity and therefore could not mandate evacuation. Conclusions Among government officials, perceived authority for hospital evacuation/shelter-in-place decision-making was viewed as a prerequisite to ordering evacuation. When both hospital executives and government officials perceive themselves to possess decision-making authority, there is the potential for inaction. Future work should examine whether a single entity bearing ultimate responsibility or regional emergency response coalitions would improve decision-making. (Disaster Med Public Health Preparedness. 2016;10:320-324) C1 [McGinty, Meghan D.; Resnick, Beth; Rutkow, Lainie] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Hlth Policy & Management, 624 N Broadway,Room 429, Baltimore, MD 21205 USA. [Barnett, Daniel J.] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Environm Hlth Sci, Baltimore, MD 21205 USA. [Smith, Katherine C.] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Hlth Behav & Soc, Baltimore, MD 21205 USA. [Burke, Thomas A.] US EPA, Washington, DC 20460 USA. RP McGinty, MD (reprint author), Johns Hopkins Bloomberg Sch Publ Hlth, Dept Hlth Policy & Management, 624 N Broadway,Room 429, Baltimore, MD 21205 USA. EM mmcginty@jhu.edu FU Johns Hopkins Environment, Energy, Sustainability, and Health Institute (E2SHI) Fellowship; Lipitz Public Health Policy Award; National Institute for Occupational Safety and Health (NIOSH) Education and Research Center (ERC) for Occupational Safety and Health at the Johns Hopkins Bloomberg School of Public Health [T42-OH008428]; Johns Hopkins Health Resources and Services Administration (HRSA) Trainee Fellowship Program Agreement [A03HP2750]; E2SHI Fellowship; John C. Hume Award; Victor Raymond Memorial Scholarship FX This research was supported by the 2013-2014 Johns Hopkins Environment, Energy, Sustainability, and Health Institute (E2SHI) Fellowship and the 2013-2014 Lipitz Public Health Policy Award. M.D.M. was supported in part by funding from the National Institute for Occupational Safety and Health (NIOSH) Education and Research Center (ERC) for Occupational Safety and Health at the Johns Hopkins Bloomberg School of Public Health (#T42-OH008428); the 2015 Johns Hopkins Health Resources and Services Administration (HRSA) Trainee Fellowship Program Agreement (#A03HP2750); the 2013-2014 E2SHI Fellowship; the 2013-2014 John C. Hume Award; and the 2014-2015 Victor Raymond Memorial Scholarship. The funders had no role in the design and conduct of the study, or collection, management, analysis, and interpretation of the data. The contents of this article are solely the responsibility of the authors and do not necessarily represent the official views of any of the funders. NR 7 TC 0 Z9 0 U1 3 U2 3 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 1935-7893 EI 1938-744X J9 DISASTER MED PUBLIC JI Dis. Med. Public Health Prep. PD JUN PY 2016 VL 10 IS 3 SI SI BP 320 EP 324 DI 10.1017/dmp.2016.86 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DQ3VD UT WOS:000379130700005 PM 27198183 ER PT J AU Inkinen, J Jayaprakash, B Domingo, JWS Keinanen-Toivola, MM Ryu, H Pitkanen, T AF Inkinen, J. Jayaprakash, B. Domingo, J. W. Santo Keinanen-Toivola, M. M. Ryu, H. Pitkanen, T. TI Diversity of ribosomal 16S DNA- and RNA-based bacterial community in an office building drinking water system SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE biofilms; drinking water; microbial community; next-generation sequencing; pipeline network ID LEGIONELLA-PNEUMOPHILA; MICROBIAL COMMUNITIES; POTABLE WATER; PYROSEQUENCING ANALYSIS; BIOFILM FORMATION; BULK WATER; SEQUENCES; COPPER; IDENTIFICATION; MYCOBACTERIA AB Aims: Next-generation sequencing of 16S ribosomal RNA genes (rDNA) and ribosomal RNA (rRNA) was used to characterize water and biofilm microbiome collected from a drinking water distribution system of an office building after its first year of operation. Methods and Results: The total bacterial community (rDNA) and active bacterial members (rRNA) sequencing databases were generated by Illumina MiSeq PE250 platform. As estimated by Chao1 index, species richness in cold water system was lower (180-260) in biofilms (Sphingomonas spp., Methylobacterium spp., Limnohabitans spp., Rhizobiales order) than in waters (250-580), (also Methylotenera spp.) (P = 0.005, n = 20). Similarly species richness (Chao1) was slightly higher (210-580) in rDNA libraries compared to rRNA libraries (150-400; P = 0.054, n = 24). Active Mycobacterium spp. was found in cross-linked polyethylene (PEX), but not in corresponding copper pipeline biofilm. Nonpathogenic Legionella spp. was found in rDNA libraries but not in rRNA libraries. Conclusions: Microbial communities differed between water and biofilms, between cold and hot water systems, locations in the building and between water rRNA and rDNA libraries, as shown by clear clusters in principal component analysis (PcoA). By using the rRNA method, we found that not all bacterial community members were active (e.g. Legionella spp.), whereas other members showed increased activity in some locations; for example, Pseudomonas spp. in hot water circulations' biofilm and order Rhizobiales and Limnohabitans spp. in stagnated locations' water and biofilm. Significance and Impact of the Study: rRNA-based methods may be better than rDNA-based methods for evaluating human health implications as rRNA methods can be used to describe the active bacterial fraction. This study indicates that copper as a pipeline material might have an adverse impact on the occurrence of Mycobacterium spp. The activity of Legionella spp. maybe questionable when detected solely by using DNA-based methods. C1 [Inkinen, J.; Keinanen-Toivola, M. M.] Satakunta Univ Appl Sci, WANDER Nord Water & Mat Inst, Fac Technol, Rauma, Finland. [Jayaprakash, B.; Pitkanen, T.] Natl Inst Hlth & Welf THL, Water & Hlth Unit, Kuopio, Finland. [Domingo, J. W. Santo; Ryu, H.; Pitkanen, T.] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. [Inkinen, J.] Aalto Univ, Sch Engn, Dept Civil Engn, POB 12100, FI-00076 Aalto, Finland. [Pitkanen, T.] Natl Inst Hlth & Welf THL, Water & Hlth Unit, POB 95, FI-70701 Kuopio, Finland. RP Inkinen, J (reprint author), Aalto Univ, Sch Engn, Dept Civil Engn, POB 12100, FI-00076 Aalto, Finland. EM jenni.inkinen@aalto.fi RI Pitkanen, Tarja/D-1657-2014 OI Pitkanen, Tarja/0000-0002-7591-9148 FU National Technology Agency of Finland (Tekes); Regional Council of Satakunta; Foundation for Aalto University Science and Technology; Foundation RTL Saatio; Kiinko Real Estate Education; High Technology Foundation of Satakunta; Osk FX The Sytytin study site has been utilized in several scientific studies, for example, RYM-SHOK (Strategic Centre for Science, Technology and Innovation of the Built Environment in Finland; Indoor Environment; Potable Water), KIITOS (Risk management of water systems in buildings), HYCTECH (Real estate's hygiene concept), Sytytin (Development of the Sytytin Technology Centre research environment). The financial support by all the participants of the above mentioned projects, especially the National Technology Agency of Finland (Tekes) and the Regional Council of Satakunta, is gratefully acknowledged. Furthermore, this research was supported in part by research grants from Foundation for Aalto University Science and Technology, Foundation RTL Saatio, Kiinko Real Estate Education, High Technology Foundation of Satakunta, Osk. Huttunen Foundation, Finnish Cultural Foundation and University of Eastern Finland. Michael Elk from US EPA, Siru Suomi at WANDER Nordic Water and Materials Institute/Prizztech Ltd. and Anna Pursiainen and Tiina Ileiskanen from National Institute for Health and Welfare (Kuopio, Finland) are acknowledged for their laboratory assistance. Kalle Riihinen from Teollisuuden Vesi Oy is acknowledged for his assistance in qPCR/NGS result comparison. Jaana Kusnetsov and Pirjo Torkko are acknowledged for their valuable help with the Legionella and Mycobacterium phylogeny respectively. Additionally, we are grateful to the city of Rauma and to the real estate company Kiinteistb Oy Rauman Sinkokatu 11 for unique research facilities. The manuscript has been subjected to the EPA's peer review and has been approved as an EPA publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by the EPA. The views expressed are those of the authors and do not necessarily reflect the views and policies of the EPA. NR 56 TC 0 Z9 0 U1 14 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD JUN PY 2016 VL 120 IS 6 BP 1723 EP 1738 DI 10.1111/jam.13144 PG 16 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DQ9MU UT WOS:000379534800025 PM 27009775 ER PT J AU Olean, TJ Gentry, JL Salter-Blanc, AJ Himmer, TM Bruno, M Tsiamis, CD AF Olean, Timothy J. Gentry, Jeff L. Salter-Blanc, Alexandra J. Himmer, Theresa M. Bruno, Morgan Tsiamis, Christos D. TI In-Canal Stabilization/Solidification of NAPL-Impacted Sediments SO REMEDIATION-THE JOURNAL OF ENVIRONMENTAL CLEANUP COSTS TECHNOLOGIES & TECHNIQUES LA English DT Article AB In situ solidification (ISS) has been used with increasing frequency as a remedial technology for source area treatment at upland sites impacted with a variety of organic contaminants, including coal tar, creosote, and other nonaqueous phase liquids (NAPLs). With several large, complex, urban water ways and rivers impacted with NAPLs, ISS is more recently being considered as a technology of choice to help reduce remedial costs, minimize short- and long-term impacts of mobile NAPL, and lower the carbon footprint. This article presents the results of a successful pilot study of ISS at the Gowanus Canal Superfund site in Brooklyn, New York. This represents the first major sediment ISS field demonstration project in a saline environment and the first project to evaluate large-scale implementation of ISS from a barge and through overlaying sediment. (C) 2016Wiley Periodicals, Inc. C1 [Olean, Timothy J.; Himmer, Theresa M.] CH2M, Boston, MA 02108 USA. [Olean, Timothy J.] CH2M, Solidificat Stabilizat Practice, Boston, MA 02108 USA. [Gentry, Jeff L.; Salter-Blanc, Alexandra J.; Bruno, Morgan] CH2M, Portland, OR USA. [Tsiamis, Christos D.] US EPA, Superfund Div, New York, NY USA. RP Olean, TJ (reprint author), CH2M, Boston, MA 02108 USA. NR 5 TC 1 Z9 1 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-5658 EI 1520-6831 J9 REMEDIATION JI Remediation PD SUM PY 2016 VL 26 IS 3 BP 9 EP 25 DI 10.1002/rem.21467 PG 17 WC Engineering, Environmental SC Engineering GA DQ8YJ UT WOS:000379497300002 ER PT J AU Loftin, KA Graham, JL Hilborn, ED Lehmann, SC Meyer, MT Dietze, JE Griffith, CB AF Loftin, Keith A. Graham, Jennifer L. Hilborn, Elizabeth D. Lehmann, Sarah C. Meyer, Michael T. Dietze, Julie E. Griffith, Christopher B. TI Cyanotoxins in inland lakes of the United States: Occurrence and potential recreational health risks in the EPA National Lakes Assessment 2007 SO HARMFUL ALGAE LA English DT Article DE Cyanotoxins; Microcystins; Cylindrospermopsins; Saxitoxins; Cyanobacteria ID NICOTINIC ACETYLCHOLINE-RECEPTORS; FRESH-WATER; CYLINDROSPERMOPSIS-RACIBORSKII; ANATOXIN-A; CYANOBACTERIA; MICROCYSTIN; IDENTIFICATION; MARINE; TOXINS; BRAZIL AB A large nation-wide survey of cyanotoxins (1161 lakes) in the United States (U.S.) was conducted during the EPA National Lakes Assessment 2007. Cyanotoxin data were compared with cyanobacteria abundance- and chlorophyll-based World Health Organization (WHO) thresholds and mouse toxicity data to evaluate potential recreational risks. Cylindrospermopsins, microcystins, and saxitoxins were detected (ELISA) in 4.0, 32, and 7.7% of samples with mean concentrations of 0.56, 3.0, and 0.061 mu g/L, respectively (detections only). Co-occurrence of the three cyanotoxin classes was rare (0.32%) when at least one toxin was detected. Cyanobacteria were present and dominant in 98 and 76% of samples, respectively. Potential anatoxin-, cylindrospermopsin-, microcystin-, and saxitoxin-producing cyanobacteria occurred in 81, 67, 95, and 79% of samples, respectively. Anatoxin-a and nodularin-R were detected (LC/MS/MS) in 15 and 3.7% samples (n= 27). The WHO moderate and high risk thresholds for microcystins, cyanobacteria abundance, and total chlorophyll were exceeded in 1.1, 27, and 44% of samples, respectively. Complete agreement by all three WHO microcystin metrics occurred in 27% of samples. This suggests that WHO microcystin metrics based on total chlorophyll and cyanobacterial abundance can overestimate microcystin risk when compared to WHO microcystin thresholds. The lack of parity among the WHO thresholds was expected since chlorophyll is common amongst all phytoplankton and not all cyanobacteria produce microcystins. Published by Elsevier B.V. C1 [Loftin, Keith A.; Meyer, Michael T.; Dietze, Julie E.; Griffith, Christopher B.] US Geol Survey, Organ Geochem Res Lab, Kansas Water Sci Ctr, Lawrence, KS 66049 USA. [Graham, Jennifer L.] US Geol Survey, Kansas Water Sci Ctr, Lawrence, KS 66049 USA. [Hilborn, Elizabeth D.] US EPA, Off Res & Dev, NHEERL, Chapel Hill, NC 27599 USA. [Lehmann, Sarah C.] US EPA, Off Wetlands Oceans & Watersheds, Ariel Rios Bldg,1200 Penn Ave NW,Mail Code 4503T, Washington, DC 20460 USA. RP Loftin, KA (reprint author), US Geol Survey, Organ Geochem Res Lab, Kansas Water Sci Ctr, Lawrence, KS 66049 USA. EM kloftin@usgs.gov; jlgraham@usgs.gov; hilborn.e@epa.gov; lehmann.sarah@epa.gov; mmeyer@usgs.gov; juliec@usgs.gov; cgriffith@usgs.gov OI Dietze, Julie/0000-0002-5936-5739 FU EPA Office of Wetlands, Oceans, and Watersheds [DW1492215401]; EPA Office of Research and Development National Health and Environmental Effects Research Laboratory [DW14958073]; USGS National Water Quality Assessment Program (NAWQA); USGS Toxic Substances Hydrology Program FX The USGS collaborated with EPA, and the U.S. states and tribes on the 2007 National Lakes Assessment to measure microcystins. USGS later expanded the scope of this study in collaboration with EPA to include measurement of cylindrospermopsins and saxitoxins to better understand the occurrence of cyanotoxin mixtures. Support was provided by the EPA Office of Wetlands, Oceans, and Watersheds (Interagency Agreement Number IA#DW1492215401), the EPA Office of Research and Development National Health and Environmental Effects Research Laboratory (IA#DW14958073), the USGS National Water Quality Assessment Program (NAWQA), and the USGS Toxic Substances Hydrology Program. The authors wish to acknowledge Bettie Kreakie, EPA and Barry Rosen, USGS for early reviews of the manuscript The views expressed in this report by the EPA authors are those of the individual authors and do not necessarily reflect the views and policies of the U.S. Environmental Protection Agency. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government.[CG] NR 83 TC 5 Z9 5 U1 26 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1568-9883 EI 1878-1470 J9 HARMFUL ALGAE JI Harmful Algae PD JUN PY 2016 VL 56 BP 77 EP 90 DI 10.1016/j.hal.2016.04.001 PG 14 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA DQ5WX UT WOS:000379277100008 PM 28073498 ER PT J AU Smith, GS Ghio, AJ Stout, JE Messier, KP Hudgens, EE Murphy, MS Pfaller, SL Maillard, JM Hilborn, ED AF Smith, Genee S. Ghio, Andrew J. Stout, Jason E. Messier, Kyle P. Hudgens, Edward E. Murphy, Mark S. Pfaller, Stacy L. Maillard, Jean-Marie Hilborn, Elizabeth D. TI Epidemiology of nontuberculous mycobacteria isolations among central North Carolina residents, 2006-2010 SO JOURNAL OF INFECTION LA English DT Article DE Nontuberculous; Mycobacteria; Mycobacterium avium complex; Environmental mycobacteria; Epidemiology; Isolation prevalence; Human ID HUMAN PULMONARY INFECTION; LUNG-DISEASE; ISOLATION PREVALENCE; FEATURES; ONTARIO AB Background: Nontuberculous mycobacteria (NTM) are environmental mycobacteria associated with a range of infections. Reports of NTM epidemiology have primarily focused on pulmonary infections and isolations, however extrapulmonary infections of the skin, soft tissues and sterile sites are less frequently described. Methods: We comprehensively reviewed laboratory reports of NTM isolation from North Carolina residents of three counties during 2006-2010. We describe age, gender, and race of patients, and anatomic site of isolation for NTM species. Results: Among 1033 patients, overall NTM isolation prevalence was 15.9/100,000 persons (13.7/100,000 excluding Mycobacterium gordonae). Prevalence was similar between genders and increased significantly with age. Extrapulmonary isolations among middle-aged black males and pulmonary isolations among elderly white females were most frequently detected. Most isolations from pulmonary sites and blood cultures were Mycobacterium avium complex; rapidly growing NTM (e.g. Mycobacterium chelonae, Mycobacterium fortuitum) were most often isolated from paranasal sinuses, wounds and skin. Conclusions: We provide the first characterization of NTM isolation prevalence in the Southeastern United States (U.S.). Variation in isolation prevalence among counties and races likely represent differences in detection, demographics and risk factors. Further characterization of NTM epidemiology is increasingly important as percentages of immunocompromised individuals and the elderly increase in the U.S. population. Published by Elsevier Ltd on behalf of The British Infection Association. C1 [Smith, Genee S.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Ghio, Andrew J.; Hudgens, Edward E.; Hilborn, Elizabeth D.] US EPA, MD 58A, Res Triangle Pk, NC 27711 USA. [Stout, Jason E.] Duke Univ, Med Ctr, Durham, NC USA. [Messier, Kyle P.] Univ N Carolina, Chapel Hill, NC USA. [Murphy, Mark S.] Innovate Inc, Alexandria, VA USA. [Pfaller, Stacy L.] US EPA, Cincinnati, OH 45268 USA. [Maillard, Jean-Marie] NC Dept Hlth & Human Serv, Raleigh, NC USA. RP Hilborn, ED (reprint author), US EPA, MD 58A, Res Triangle Pk, NC 27711 USA. EM hilborn.e@epa.gov OI Stout, Jason/0000-0002-6698-8176 FU U.S. Environmental Protection Agency [EP13D000041, DW89922983] FX We thank those who kindly assisted with data access: Katherine Link, Duke University Medical Center, Durham, North Carolina; Barbara Body, LabCorp, Burlington, North Carolina; Ellen Fortenberry and Mitzi Kelbaugh, North Carolina Department of Health and Human Services, Raleigh, North Carolina; Diane Powers, Jason Elder and Nathan Mize, Rex Hospital, Raleigh, North Carolina; Melissa Miller, Alan Kerr and Andrea Reed, University of North Carolina Hospitals, Chapel Hill, North Carolina; Janice Kloppenburg, Quest Diagnostics, Charlotte, North Carolina. This work was supported by U.S. Environmental Protection Agency [Contract #EP13D000041; IA#DW89922983]. NR 30 TC 2 Z9 2 U1 0 U2 1 PU W B SAUNDERS CO LTD PI LONDON PA 32 JAMESTOWN RD, LONDON NW1 7BY, ENGLAND SN 0163-4453 EI 1532-2742 J9 J INFECTION JI J. Infect. PD JUN PY 2016 VL 72 IS 6 BP 678 EP 686 DI 10.1016/j.jinf.2016.03.008 PG 9 WC Infectious Diseases SC Infectious Diseases GA DQ3RK UT WOS:000379119600005 PM 26997636 ER PT J AU Mapp, L Chambers, Y Takundwa, P Hill, VR Schneeberger, C Knee, J Raynor, M Klonicki, P Miller, K Pope, M Hwang, N AF Mapp, Latisha Chambers, Yildiz Takundwa, Prisca Hill, Vincent R. Schneeberger, Chandra Knee, Jackie Raynor, Malik Klonicki, Patricia Miller, Kenneth Pope, Misty Hwang, Nina TI Inter-Laboratory Evaluation and Successful Implementation of MS2 Coliphage as a Surrogate to Establish Proficiency Using a BSL-3 Procedure SO WATER LA English DT Article DE male-specific (MS2); coliphage; drinking water; quality control criteria; ultrafiltration ID TAP WATER SAMPLES; WASTE-WATER; SIMULTANEOUS RECOVERY; DRINKING-WATER; ULTRAFILTRATION; DISINFECTION; GROUNDWATER; MICROBES; REMOVAL; SCALE AB The U.S. Environmental Protection Agency's (EPA) Water Laboratory Alliance relies on the Centers for Disease Control and Prevention's ultrafiltration-based Water Processing Procedure (WPP) for concentration of biosafety level 3 (BSL-3) agents from 10 L to 100 L of drinking water. The WPP requires comprehensive training and practice to maintain proficiency, resulting in a critical need for quality control (QC) criteria. The aim of this study was to develop criteria using male-specific (MS2) coliphage (BSL-2 agent) to minimize safety hazards associated with BSL-3 agents and to use the criteria to evaluate analytical proficiency during a demonstration exercise. EPA Method 1602 with EasyPhage was used during the study to develop QC criteria for 100-mL, and 40-100 L samples. The demonstration exercise indicated that the MS2 criteria would allow laboratories to demonstrate proficiency using the WPP with 40-100 L samples. In addition, the QC criteria developed for 100-mL samples has broad applicability at laboratories that are using MS2 for other types of analyses, such as assessment of water treatment devices. The development of MS2 QC criteria allows laboratories to develop and confirm ongoing proficiency using the WPP. C1 [Mapp, Latisha; Takundwa, Prisca; Raynor, Malik; Hwang, Nina] US EPA, Off Water, 1200 Penn Ave NW, Washington, DC 20460 USA. [Chambers, Yildiz; Klonicki, Patricia; Miller, Kenneth; Pope, Misty] CSRA, Sci & Engn, 6361 Walker Lane,Suite 300, Alexandria, VA 22310 USA. [Hill, Vincent R.] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, 1600 Clifton Rd NE, Atlanta, GA 30333 USA. [Schneeberger, Chandra; Knee, Jackie] IHRC Inc, 2 Ravinia Dr NE, Atlanta, GA 30346 USA. RP Hill, VR (reprint author), Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, 1600 Clifton Rd NE, Atlanta, GA 30333 USA. EM mapp.latisha@epa.gov; yildiz.chambers@csra.com; tendait@gmail.com; vhill@cdc.gov; cschneeberger@cdc.gov; jackie.knee@gmail.com; Malik.J.Raynor@uth.tmc.edu; tricia.klonicki@csra.com; kenneth.m.miller@csra.com; misty.pope@csra.com; nina.hwang12@gmail.com FU EPA's Office of Ground Water and Drinking Water, Water Security Division [DW-75-922304801]; CDC Office of Public Health Preparedness and Response FX Contributions across a wide range of issues are gratefully acknowledged from the following subject matter experts and environmental/public health laboratories: Erin Black, Mike Bowen, Jasmine Chaitram, Bruce Newton, Todd Parker, and Beth Schweitzer (U.S. CDC); Mike Tyree, Bill Fromme and David Hartman (GCWW); Mike Chicoine (Washington Aqueduct); Melissa Billman and Meg Carlson (Fairfax CountyWater Authority); Mark Meckes and Laura Boczek (EPA NRMRL); and Sharon Long, Sharon Kluender, and Jeremy Olstadt (Wisconsin State Laboratory of Hygiene). This work was supported by the EPA's Office of Ground Water and Drinking Water, Water Security Division under Interagency Agreement DW-75-922304801. We gratefully acknowledge the contributions of Erin Silverstri (EPA National Homeland Security Research Center) in facilitating this partnership, as well as her contributions to our planning workgroup and review of this manuscript. Additional support was provided by CDC Office of Public Health Preparedness and Response. The findings and conclusions in this report are those of the authors and should not be construed to represent any agency determination or policy. Use of trade names and commercial sources is for identification only and does not imply endorsement by the U.S. CDC or the U.S. EPA. NR 27 TC 0 Z9 0 U1 1 U2 1 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4441 J9 WATER-SUI JI Water PD JUN PY 2016 VL 8 IS 6 AR 248 DI 10.3390/w8060248 PG 14 WC Water Resources SC Water Resources GA DP9XN UT WOS:000378851300032 ER PT J AU Pleil, J Risby, T Herbig, J AF Pleil, Joachim Risby, Terence Herbig, Jens TI Breath biomonitoring in national security assessment, forensic THC testing, biomedical technology and quality assurance applications: report from PittCon 2016 SO JOURNAL OF BREATH RESEARCH LA English DT Article DE Pittsburgh conference; toxicology; forensics; national security C1 [Pleil, Joachim] US EPA, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Risby, Terence] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Baltimore, MD USA. [Herbig, Jens] Ionicon Analyt, Innsbruck, Austria. RP Pleil, J (reprint author), US EPA, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. EM pleil.joachim@epa.gov; trisby1@jhu.edu; jens.herbig@ionicon.com NR 6 TC 0 Z9 0 U1 1 U2 3 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1752-7155 EI 1752-7163 J9 J BREATH RES JI J. Breath Res. PD JUN PY 2016 VL 10 IS 2 AR 029001 DI 10.1088/1752-7155/10/2/029001 PG 3 WC Biochemical Research Methods; Respiratory System SC Biochemistry & Molecular Biology; Respiratory System GA DO9GA UT WOS:000378091800017 PM 27137650 ER PT J AU Ma, JW Feng, YY Hu, Y Villegas, EN Xiao, LH AF Ma, Jiawen Feng, Yaoyu Hu, Yue Villegas, Eric N. Xiao, Lihua TI Human infective potential of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in urban wastewater treatment plant effluents SO JOURNAL OF WATER AND HEALTH LA English DT Article DE Cryptosporidium; Enterocytozoon bieneusi; genotype; Giardia; wastewater; WWTP effluent ID CHLORINE DIOXIDE; HEALTH-RISK; MOLECULAR EPIDEMIOLOGY; SURFACE-WATER; OOCYSTS; IDENTIFICATION; MICROSPORIDIA; PARVUM; GENOTYPES; ANIMALS AB Cryptosporidiosis, giardiasis, and microsporidiosis are important waterborne diseases. In the standard for wastewater treatment plant (WWTP) effluents in China and other countries, the fecal coliform count is the only microbial indicator, raising concerns about the potential for pathogen transmission through WWTP effluent reuse. In this study, we collected 50 effluent samples (30 L/sample) from three municipal WWTPs in Shanghai, China, and analyzed for Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi by microscopy and/or polymerase chain reaction (PCR). Moreover, propidium monoazide (PMA)-PCR was used to assess the viability of oocysts/cysts. The microscopy and PCR-positive rates for Cryptosporidium spp. were 62% and 40%, respectively. The occurrence rates of G. duodenalis were 96% by microscopy and 92-100% by PCR analysis of three genetic loci. Furthermore, E. bieneusi was detected in 70% (35/50) of samples by PCR. Altogether, 10 Cryptosporidium species or genotypes, two G. duodenalis genotypes, and 11 E. bieneusi genotypes were found, most of which were human-pathogenic. The chlorine dioxide disinfection employed in WWTP1 and WWTP3 failed to inactivate the residual pathogens; 93% of the samples from WWTP1 and 83% from WWTP3 did not meet the national standard on fecal coliform levels. Thus, urban WWTP effluents often contain residual waterborne human pathogens. C1 [Ma, Jiawen; Feng, Yaoyu; Hu, Yue] E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China. [Villegas, Eric N.] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [Xiao, Lihua] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30329 USA. RP Feng, YY (reprint author), E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China. EM yyfeng@ecust.edu.cn RI Feng, Yaoyu/B-3076-2014; Xiao, Lihua/B-1704-2013 OI Xiao, Lihua/0000-0001-8532-2727 FU National Natural Science Foundation of China [31425025, 31229005]; Water Special Project of the Ministry of Science and Technology, China [2014ZX07104006]; Fundamental Research Funds for the Central Universities, China FX This work was supported by National Natural Science Foundation of China (31425025 and 31229005), Water Special Project of the Ministry of Science and Technology, China (2014ZX07104006), and Fundamental Research Funds for the Central Universities, China. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention or the US Environmental Protection Agency. The work has been subjected to Agency review and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 58 TC 2 Z9 2 U1 7 U2 13 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 1477-8920 J9 J WATER HEALTH JI J. Water Health PD JUN PY 2016 VL 14 IS 3 BP 411 EP 423 DI 10.2166/wh.2016.192 PG 13 WC Environmental Sciences; Public, Environmental & Occupational Health; Microbiology; Water Resources SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Microbiology; Water Resources GA DO5HH UT WOS:000377813700005 PM 27280607 ER PT J AU Kim, K Whelan, G Molina, M Purucker, ST Pachepsky, Y Guber, A Cyterski, MJ Franklin, DH Blaustein, RA AF Kim, Keewook Whelan, Gene Molina, Marirosa Purucker, S. Thomas Pachepsky, Yakov Guber, Andrey Cyterski, Michael J. Franklin, Dorcas H. Blaustein, Ryan A. TI Rainfall-induced release of microbes from manure: model development, parameter estimation, and uncertainty evaluation on small plots SO JOURNAL OF WATER AND HEALTH LA English DT Article DE bootstrap; Bradford-Schijven release model; manure; microbe; PEST; quantitative microbial risk assessment (QMRA) ID ESCHERICHIA-COLI; E. COLI; DIE-OFF; SURVIVAL; ENTEROCOCCI; BACTERIA; CRYPTOSPORIDIUM; TRANSPORT; COWPATS; GIARDIA AB A series of simulated rainfall-runoff experiments with applications of different manure types (cattle solid pats, poultry dry litter, swine slurry) was conducted across four seasons on a field containing 36 plots (0.75 x 2 m each), resulting in 144 rainfall-runoff events. Simulating time-varying release of Escherichia coli, enterococci, and fecal coliforms from manures applied at typical agronomic rates evaluated the efficacy of the Bradford-Schijven model modified by adding terms for release efficiency and transportation loss. Two complementary, parallel approaches were used to calibrate the model and estimate microbial release parameters. The first was a four-step sequential procedure using the inverse model PEST, which provides appropriate initial parameter values. The second utilized a PEST/bootstrap procedure to estimate average parameters across plots, manure age, and microbe, and to provide parameter distributions. The experiment determined that manure age, microbe, and season had no clear relationship to the release curve. Cattle solid pats released microbes at a different, slower rate than did poultry dry litter or swine slurry, which had very similar release patterns. These findings were consistent with other published results for both bench-and field-scale, suggesting the modified Bradford-Schijven model can be applied to microbial release from manure. C1 [Kim, Keewook] US DOE, Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37830 USA. [Kim, Keewook] Univ Idaho, Idaho Falls Ctr Higher Educ, Idaho Falls, ID 83402 USA. [Kim, Keewook; Whelan, Gene; Molina, Marirosa; Purucker, S. Thomas; Cyterski, Michael J.] US EPA, Natl Exposure Res Lab, Ecosyst Res Div, Athens, GA 30605 USA. [Pachepsky, Yakov] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Guber, Andrey] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Franklin, Dorcas H.] ARS, USDA, Watkinsville, GA 30677 USA. [Franklin, Dorcas H.] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. [Blaustein, Ryan A.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. RP Kim, K (reprint author), US DOE, Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37830 USA.; Kim, K (reprint author), Univ Idaho, Idaho Falls Ctr Higher Educ, Idaho Falls, ID 83402 USA. EM kkeewook@uidaho.edu FU United States Environmental Protection Agency through its Office of Research and Development; Office of Water; Office of Research and Development FX The United States Environmental Protection Agency through its Office of Research and Development and in collaboration with the Office of Water funded and managed the research described here. It has been subjected to Agency review and approved for publication. This research was supported in part by an appointment to the Research Participation Program at the United States Environmental Protection Agency, Office of Research and Development, administered by the Oak Ridge Institute for Science and Education through Interagency Agreement No. DW8992298301 between the United States Department of Energy and the United States Environmental Protection Agency. The Richard B. Russell Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, in Athens, GA provided technical assistance on the field work. Data collection and collation services were provided by students under contract with the Student Services Contracting Authority. NR 40 TC 0 Z9 0 U1 3 U2 4 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 1477-8920 J9 J WATER HEALTH JI J. Water Health PD JUN PY 2016 VL 14 IS 3 BP 443 EP 459 DI 10.2166/wh.2016.239 PG 17 WC Environmental Sciences; Public, Environmental & Occupational Health; Microbiology; Water Resources SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Microbiology; Water Resources GA DO5HH UT WOS:000377813700008 PM 27280610 ER PT J AU Liu, JQ Chen, HY Huang, Q Lou, LP Hu, BL Endalkachew, SD Mallikarjuna, N Shan, YG Zhou, XY AF Liu, Jingqing Chen, Huanyu Huang, Qian Lou, Liping Hu, Baolan Endalkachew, Sahle-Demessie Mallikarjuna, Nadagouda Shan, Yonggui Zhou, Xiaoyan TI Characteristics of pipe-scale in the pipes of an urban drinking water distribution system in eastern China SO WATER SCIENCE AND TECHNOLOGY-WATER SUPPLY LA English DT Article DE contaminants; drinking water distribution system; heavy metal; organic pollutants; pipe-scale ID IRON CORROSION SCALES; BY-PRODUCT RELEASE; FATTY-ACID AMIDES; ORGANIC-MATTER; OFF-FLAVORS; BIOFILM; COPPER; LEAD; METALS; TOXICITY AB Pipes that carry drinking water have gradually aged. Events occurring with increasing frequency, such as substandard water quality in residential taps, red water, and black water, reveal the deterioration of the chemical stability of a drinking water distribution system (DWDS). Pipes in the DWDS serving City S, located in eastern China, were sampled to analyze the concentration and distribution of pollutants in pipe-scale of pipes of different materials, ages and diameters, and the factors (such as materials, age, and diameter) influencing the accumulation of pollutants were also investigated. The quantity of pipe-scale in the most commonly used gray cast iron pipe and ductile cast iron pipe (DN150) was 151.5-195.0 g.m(-1) and 7.1-29.4 g.m(-1), respectively. The concentration of heavy metals in pipe-scale was positively correlated with the quantity of pipe-scale (R-2 = 0.874); the sequence of concentration of metals was Fe > Al > Mn, Zn > Pb, Cu > Cr, Cd. Galvanized steel pipe, with the highest degree of corrosion, had the highest concentration of heavy metals in pipe-scale. The morphology and composition of pipe-scale were substantially influenced by pipe material and age. For example, in the oldest galvanized steel pipe-scale, there was not only a large number of iron compounds but also some zinc composite oxides. In addition to hydrocarbons produced by microbial metabolism, there were microalgae metabolites and exogenous contaminants such as polycyclic aromatic hydrocarbons (PAHs). The concentrations of microbial metabolites increased with increasing service time. C1 [Liu, Jingqing] Zhejiang Univ, Coll Engn & Architecture, Hangzhou 310058, Zhejiang, Peoples R China. [Chen, Huanyu; Huang, Qian; Lou, Liping; Hu, Baolan] Zhejiang Univ, Coll Environm & Resource Sci, Hangzhou 310058, Zhejiang, Peoples R China. [Endalkachew, Sahle-Demessie; Mallikarjuna, Nadagouda; Shan, Yonggui] US EPA, Off Res & Dev, NRMRL, Cincinnati, OH 45220 USA. [Zhou, Xiaoyan] Shaoxing Water Environm Sci Inst Co Ltd, Shaoxing 312000, Peoples R China. [Chen, Huanyu] Zhejiang Univ, Binhai Ind Technol Res Inst, Tianjin 300020, Peoples R China. RP Lou, LP (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, Hangzhou 310058, Zhejiang, Peoples R China. EM loulp@zju.edu.cn FU Key Special Program on the S&T Pollution Control and Treatment of Water Bodies [2012ZX07403-003]; National Key Technology RD Program [2012BAJ25B07] FX The authors thank the Key Special Program on the S&T Pollution Control and Treatment of Water Bodies (no. 2012ZX07403-003), the National Key Technology R&D Program (no. 2012BAJ25B07) and the National Natural Science Foundation of China (no. 51378455). NR 43 TC 0 Z9 0 U1 16 U2 18 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 1606-9749 J9 WATER SCI TECH-W SUP JI Water Sci. Technol.-Water Supply PD JUN PY 2016 VL 16 IS 3 BP 715 EP 726 DI 10.2166/ws.2015.183 PG 12 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DO5GR UT WOS:000377812100015 ER PT J AU Esmond, NG de la Cruz, AA Ghosh, D Stelma, G Bernstein, JA AF Esmond, N. Geh de la Cruz, Armah A. Ghosh, Debajyoti Stelma, Gerard Bernstein, Jonathan A. TI Sensitization of a child to Cyanobacteria after recreational swimming in a lake SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Letter ID TRANSCRIPTION FACTOR; IMMUNE-RESPONSES; IL-9 PRODUCTION; MURINE MODEL; MAST-CELLS; ASTHMA; LYMPHOCYTE; ALLERGEN; DIFFERENTIATION; IDENTIFICATION C1 [Esmond, N. Geh; Ghosh, Debajyoti; Bernstein, Jonathan A.] Univ Cincinnati, Dept Internal Med, Immunol & Allergy, Cincinnati, OH USA. [Esmond, N. Geh] Univ Cincinnati, Dept Environm Hlth, Cincinnati, OH USA. [de la Cruz, Armah A.; Stelma, Gerard] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. RP Bernstein, JA (reprint author), Univ Cincinnati, Dept Internal Med, Immunol & Allergy, Cincinnati, OH USA. EM Jonathan.Bernstein@uc.edu NR 28 TC 0 Z9 0 U1 1 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD JUN PY 2016 VL 137 IS 6 BP 1902 EP + DI 10.1016/j.jaci.2015.12.1340 PG 10 WC Allergy; Immunology SC Allergy; Immunology GA DO1GT UT WOS:000377527200038 ER PT J AU Angradi, TR Launspach, JJ Bolgrien, DW Bellinger, BJ Starry, MA Hoffman, JC Trebitz, AS Sierszen, ME Hollenhorst, TP AF Angradi, Ted R. Launspach, Jonathon J. Bolgrien, David W. Bellinger, Brent J. Starry, Matthew A. Hoffman, Joel C. Trebitz, Anett S. Sierszen, Mike E. Hollenhorst, Tom P. TI Mapping ecosystem service indicators in a Great Lakes estuarine Area of Concern SO JOURNAL OF GREAT LAKES RESEARCH LA English DT Article DE Ecosystem services; St. Louis River; Estuary; Great Lakes Area of Concern; Decision-making; Habitat restoration ID VALUES; CHALLENGES; MANAGEMENT; STRESSORS; IMPLEMENTATION; WORLD AB Estuaries provide multiple ecosystem services from which humans benefit. Twenty-seven Great Lakes coastal systems in the United States and Canada, many of them estuarine, are currently designated as Areas of Concern (AOCs) due to a legacy of chemical contamination, degraded habitat, and non-point-source pollution. The ecosystem benefits that current and future human communities can receive from these degraded ecosystems are diminished. For an AOC to be delisted, it is generally necessary to restore aquatic habitat, among other actions. Ecosystem service mapping and assessment can inform AOC restoration and management. We describe an approach, with examples, for assessing how local-scale actions affect the extent and distribution of coastal ecosystem services, using the estuarine portion of St. Louis River AOC of western Lake Superior as a case study. We applied mapping criteria derived from locally validated predictive models, published relationships, local experts, and other sources to spatially explicit biophysical data to map indicators of ecosystem services at high resolution across aquatic and riparian habitats. We mapped indicators for 23 biophysical services in the estuary (e.g., natural views, boating, game and non-game fish and wildlife species, wild rice, parks and trails, beaches, property protection, sacred sites). Most 10 x 10 m aquatic pixel locations had 3-7 overlapping services; riparian locations had fewer mapped services. To examine tradeoffs in services associated with management actions, we quantified the changes in the area of the AOC providing each ecosystem service for scenarios based on planned sediment remediation and habitat restoration projects. Some aspects of our approach can be adapted by Great Lakes coastal communities to support local environmental decision-making. Published by Elsevier B.V. on behalf of International Association for Great Lakes Research. All rights reserved. C1 [Angradi, Ted R.; Bolgrien, David W.; Hoffman, Joel C.; Trebitz, Anett S.; Sierszen, Mike E.; Hollenhorst, Tom P.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Midcontinent Ecol Div, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Launspach, Jonathon J.] SRA Int Inc, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Bellinger, Brent J.] City Austin Watershed Protect Dept, 505 Barton Springs Rd 11th Floor, Austin, TX 78704 USA. [Starry, Matthew A.] Super Water Light & Power, 2915 Hill Ave, Superior, WI 54880 USA. RP Angradi, TR (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Midcontinent Ecol Div, 6201 Congdon Blvd, Duluth, MN 55804 USA. EM angradi.theodore@epa.gov NR 48 TC 0 Z9 0 U1 13 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0380-1330 J9 J GREAT LAKES RES JI J. Gt. Lakes Res. PD JUN PY 2016 VL 42 IS 3 BP 717 EP 727 DI 10.1016/j.jglr.2016.03.012 PG 11 WC Environmental Sciences; Limnology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DO1RL UT WOS:000377556700024 ER PT J AU Cotter, KA Nacci, D Champlin, D Yeo, AT Gilmore, TD Callard, GV AF Cotter, Kellie A. Nacci, Diane Champlin, Denise Yeo, Alan T. Gilmore, Thomas D. Callard, Gloria V. TI Adaptive Significance of ER alpha Splice Variants in Killifish (Fundulus heteroclitus) Resident in an Estrogenic Environment SO ENDOCRINOLOGY LA English DT Article ID EPIGENETIC TRANSGENERATIONAL INHERITANCE; ENDOCRINE-DISRUPTING CHEMICALS; MESSENGER-RNA VARIANTS; MARINE SUPERFUND SITE; ATLANTIC KILLIFISH; TISSUE-TYPE; DIFFERENTIAL EXPRESSION; UNPOLLUTED ENVIRONMENTS; SCIENTIFIC STATEMENT; DNA METHYLATION AB The possibility that chronic, multigenerational exposure to environmental estrogens selects for adaptive hormone-response phenotypes is a critical unanswered question. Embryos/larvae of killifish from an estrogenic-polluted environment (New Bedford Harbor, MA [NBH]) compared with those from a reference site overexpress estrogen receptor alpha (ERa) mRNA but are hyporesponsive to estradiol. Analysis of ERa mRNAs in the two populations revealed differences in splicing of the gene encoding ERa (esr1). Here we tested the transactivation functions of four differentially expressed ERa mRNAs and tracked their association with the hyporesponsive phenotype for three generations after transfer of NBH parents to a clean environment. Deletion variants ERa Delta 6 and ERa Delta 6-8 were specific to NBH killifish, had dominant negative functions in an in vitro reporter assay, and were heritable. Morpholino-mediated induction of ERa Delta 6 mRNA in zebrafish embryos verified its role as a dominant negative ER on natural estrogen-responsive promoters. Alternate long (ERaL) and short (ERaS) 5'-variants were similar transcriptionally but differed in estrogen responsiveness (ERaS >> ERaL). ERaS accounted for high total ER alpha expression in first generation (F1) NBH embryos/larvae but this trait was abolished by transfer to clean water. By contrast, the hyporesponsive phenotype of F1 NBH embryos/larvae persisted after long-term laboratory holding but reverted to a normal or hyper-responsive phenotype after two or three generations, suggesting the acquisition of physiological or biochemical traits that compensate for ongoing expression of negative-acting ERa Delta 6 and ERa Delta 6-8 isoforms. We conclude that a heritable change in the pattern of alternative splicing of ERapre-mRNA is part of a genetic adaptive response to estrogens in a polluted environment. C1 [Cotter, Kellie A.; Yeo, Alan T.; Gilmore, Thomas D.; Callard, Gloria V.] Boston Univ, Dept Biol, 5 Cummington Mall, Boston, MA 02215 USA. [Nacci, Diane; Champlin, Denise] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Atlantic Ecol Div, Narragansett, RI 02882 USA. RP Callard, GV (reprint author), Boston Univ, Dept Biol, 5 Cummington Mall, Boston, MA 02215 USA. EM gvc@bu.edu FU National Institutes for Environmental Health Sciences (NIEHS) [P42ES07381]; U.S. Environmental Protection Agency National Center for Environmental Research Science to Achieve Results (STAR) Program [RD831301]; Boston University; Warren-McLeod Fellowship for Graduate Research in Marine Biology; EPA STAR Fellowship [FP917445]; National Science Foundation [DEB-12652821] FX This work was supported by the National Institutes for Environmental Health Sciences (NIEHS P42ES07381), the U.S. Environmental Protection Agency National Center for Environmental Research Science to Achieve Results (STAR) Program (RD831301), a Boston University, Warren-McLeod Fellowship for Graduate Research in Marine Biology (K.A.C.); an EPA STAR Fellowship (FP917445; K.A.C.); and a National Science Foundation grant (DEB-12652821; PI, Andrew Whitehead, UC Davis) in support of the killifish genome project. NR 53 TC 0 Z9 0 U1 5 U2 11 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD JUN PY 2016 VL 157 IS 6 BP 2294 EP 2308 DI 10.1210/en.2016-1052 PG 15 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DN6VM UT WOS:000377214600011 PM 27070100 ER PT J AU Egeghy, PP Sheldon, LS Isaacs, KK Ouml;zkaynak, H Goldsmith, MR Wambaugh, JF Judson, RS Buckley, TJ AF Egeghy, Peter P. Sheldon, Linda S. Isaacs, Kristin K. Ozkaynak, Haluk Goldsmith, Michael-Rock Wambaugh, John F. Judson, Richard S. Buckley, Timothy J. TI Computational Exposure Science: An Emerging Discipline to Support 21st-Century Risk Assessment SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID IN-VITRO BIOACTIVITY; CHEMICAL-EXPOSURE; PRIORITIZATION; MODELS; TOOL; POLLUTANTS; TOXICOLOGY; CHALLENGE; PARADIGM; EXPOSOME AB BACKGROUND: Computational exposure science represents a frontier of environmental science that is emerging and quickly evolving. OBJECTIVES: In this commentary, we define this burgeoning discipline, describe a framework for implementation, and review some key ongoing research elements that are advancing the science with respect to exposure to chemicals in consumer products. DISCUSSION: The fundamental elements of computational exposure science include the development of reliable, computationally efficient predictive exposure models; the identification, acquisition, and application of data to support and evaluate these models; and generation of improved methods for extrapolating across chemicals. We describe our efforts in each of these areas and provide examples that demonstrate both progress and potential. CONCLUSIONS: Computational exposure science, linked with comparable efforts in toxicology, is ushering in a new era of risk assessment that greatly expands our ability to evaluate chemical safety and sustainability and to protect public health. C1 [Egeghy, Peter P.; Sheldon, Linda S.; Isaacs, Kristin K.; Ozkaynak, Haluk; Goldsmith, Michael-Rock; Wambaugh, John F.; Judson, Richard S.; Buckley, Timothy J.] US EPA, Off Res & Dev, 109 TW Alexander Dr,Mail Code E205-04, Res Triangle Pk, NC 27711 USA. [Goldsmith, Michael-Rock] Chem Comp Grp, Montreal, PQ, Canada. RP Egeghy, PP (reprint author), US EPA, Off Res & Dev, 109 TW Alexander Dr,Mail Code E205-04, Res Triangle Pk, NC 27711 USA. EM egeghy.peter@epa.gov OI Wambaugh, John/0000-0002-4024-534X NR 61 TC 3 Z9 3 U1 17 U2 35 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD JUN PY 2016 VL 124 IS 6 BP 697 EP 702 DI 10.1289/ehp.1509748 PG 6 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DN5BO UT WOS:000377081300011 PM 26545029 ER PT J AU Li, Z Hogan, KA Cai, C Rieth, S AF Li, Zheng Hogan, Karen A. Cai, Christine Rieth, Susan TI Human Health Effects of Biphenyl: Key Findings and Scientific Issues SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Review ID ISOLATED RAT HEPATOCYTES; UNSCHEDULED DNA-SYNTHESIS; IN-VIVO GENOTOXICITY; MALE F344 RATS; ORTHO-PHENYLPHENOL; SALMONELLA-TYPHIMURIUM; PHENOLIC METABOLITES; CELL-PROLIFERATION; URINARY-BLADDER; O-PHENYLPHENOL AB BACKGROUND: In support of the Integrated Risk Information System (IRIS), the U.S. Environmental Protection Agency (EPA) has evaluated the human health hazards of biphenyl exposure. OBJECTIVES: We review key findings and scientific issues regarding expected human health effects of biphenyl. METHODS: Scientific literature from 1926 through September 2012 was critically evaluated to identify potential human health hazards associated with biphenyl exposure. Key issues related to the carcinogenicity and noncancer health hazards of biphenyl were examined based on evidence from experimental animal bioassays and mechanistic studies. DISCUSSION: Systematic consideration of experimental animal studies of oral biphenyl exposure took into account the variety of study designs (e.g., study sizes, exposure levels, and exposure durations) to reconcile differing reported results. The available mechanistic and toxicokinetic evidence supports the hypothesis that male rat urinary bladder tumors arise through urinary bladder calculi formation but is insufficient to hypothesize a mode of action for liver tumors in female mice. Biphenyl and its metabolites may induce genetic damage, but a role for genotoxicity in biphenyl-induced carcinogenicity has not been established. CONCLUSIONS: The available health effects data for biphenyl provides suggestive evidence for carcino-genicity in humans, based on increased incidences of male rat urinary bladder tumors at high exposure levels and on female mouse liver tumors. Kidney toxicity is also a potential human health hazard of biphenyl exposure. C1 [Li, Zheng; Hogan, Karen A.; Cai, Christine; Rieth, Susan] US EPA, Natl Ctr Environm Assessment, Washington, DC 20460 USA. RP Li, Z (reprint author), US EPA, Natl Ctr Environm Assessment 8601P, Two Potomac Yard North Bldg,2733 S Crystal Dr, Arlington, VA 22202 USA. EM li.jenny@epa.gov NR 107 TC 0 Z9 0 U1 6 U2 14 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD JUN PY 2016 VL 124 IS 6 BP 703 EP 712 DI 10.1289/ehp.1509730 PG 10 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DN5BO UT WOS:000377081300012 PM 26529796 ER PT J AU Smith, MT Guyton, KZ Gibbons, CF Fritz, JM Portier, CJ Rusyn, I DeMarini, DM Caldwell, JC Kavlock, RJ Lambert, PF Hecht, SS Bucher, JR Stewart, BW Baan, RA Cogliano, VJ Straif, K AF Smith, Martyn T. Guyton, Kathryn Z. Gibbons, Catherine F. Fritz, Jason M. Portier, Christopher J. Rusyn, Ivan DeMarini, David M. Caldwell, Jane C. Kavlock, Robert J. Lambert, Paul F. Hecht, Stephen S. Bucher, John R. Stewart, Bernard W. Baan, Robert A. Cogliano, Vincent J. Straif, Kurt TI Key Characteristics of Carcinogens as a Basis for Organizing Data on Mechanisms of Carcinogenesis SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Review ID BENZENE-INDUCED LEUKEMIA; LOW-DOSE EXPOSURES; EPIGENETIC MECHANISMS; MOLECULAR-MECHANISMS; GENOMIC INSTABILITY; SIGNALING PATHWAYS; CHEMICAL-MIXTURES; NUCLEAR RECEPTORS; DRUG-METABOLISM; CANCER HALLMARK AB BACKGROUND: A recent review by the International Agency for Research on Cancer (IARC) updated the assessments of the > 100 agents classified as Group 1, carcinogenic to humans (IARC Monographs Volume 100, parts A-F). This exercise was complicated by the absence of a broadly accepted, systematic method for evaluating mechanistic data to support conclusions regarding human hazard from exposure to carcinogens. OBJECTIVES AND METHODS: IARC therefore convened two workshops in which an international Working Group of experts identified 10 key characteristics, one or more of which are commonly exhibited by established human carcinogens. DISCUSSION: These characteristics provide the basis for an objective approach to identifying and organizing results from pertinent mechanistic studies. The 10 characteristics are the abilities of an agent to 1) act as an electrophile either directly or after metabolic activation; 2) be genotoxic; 3) alter DNA repair or cause genomic instability; 4) induce epigenetic alterations; 5) induce oxidative stress; 6) induce chronic inflammation; 7) be immunosuppressive; 8) modulate -receptor-mediated effects; 9) cause immortalization; and 10) alter cell proliferation, cell death, or nutrient supply. CONCLUSION: We describe the use of the 10 key characteristics to conduct a systematic literature search focused on relevant end points and construct a graphical representation of the identified mechanistic information. Next, we use benzene and polychlorinated biphenyls as examples to illustrate how this approach may work in practice. The approach described is similar in many respects to those currently being implemented by the U.S. EPA's Integrated Risk Information System Program and the U.S. National Toxicology Program. C1 [Smith, Martyn T.] Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Room 386, Berkeley, CA 94720 USA. [Guyton, Kathryn Z.; Baan, Robert A.; Straif, Kurt] Int Agcy Res Canc, 150 Cours Albert Thomas, F-69372 Lyon, France. [Gibbons, Catherine F.; Fritz, Jason M.; DeMarini, David M.; Caldwell, Jane C.; Kavlock, Robert J.; Cogliano, Vincent J.] US EPA, Off Res & Dev, Washington, DC 20460 USA. [Gibbons, Catherine F.; Fritz, Jason M.; DeMarini, David M.; Caldwell, Jane C.; Kavlock, Robert J.; Cogliano, Vincent J.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Portier, Christopher J.] Environm Def Fund, Washington, DC USA. [Rusyn, Ivan] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Integrat Biosci, College Stn, TX USA. [Lambert, Paul F.] Univ Wisconsin, Sch Med & Publ Hlth, McArdle Lab Canc Res, Madison, WI USA. [Hecht, Stephen S.] Univ Minnesota, Masonic Canc Ctr, Minneapolis, MN USA. [Bucher, John R.] NIEHS, Natl Toxicol Program, NIH, US Dept HHS, POB 12233, Res Triangle Pk, NC 27709 USA. [Stewart, Bernard W.] Univ New S Wales, Fac Med, Sydney, NSW, Australia. RP Smith, MT (reprint author), Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Li Ka Shing Ctr, Room 386, Berkeley, CA 94720 USA. EM martynts@berkeley.edu RI Fritz, Jason/K-1439-2013; Rusyn, Ivan/S-2426-2016 FU National Institutes of Health, National Institute of Environmental Health Sciences [P42ES004705]; attorneys FX M.T.S. was supported by National Institutes of Health, National Institute of Environmental Health Sciences grant P42ES004705.; M. T. S. has received consulting fees from attorneys representing plaintiffs and defense in cases involving exposure to benzene and other chemical agents. The other authors declare they have no actual or potential competing financial interests. NR 73 TC 12 Z9 12 U1 8 U2 21 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD JUN PY 2016 VL 124 IS 6 BP 713 EP 721 DI 10.1289/ehp.1509912 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DN5BO UT WOS:000377081300013 PM 26600562 ER PT J AU Karmaus, AL Filer, DL Martin, MT Houck, KA AF Karmaus, Agnes L. Filer, Dayne L. Martin, Matthew T. Houck, Keith A. TI Evaluation of food-relevant chemicals in the ToxCast high-throughput screening program SO FOOD AND CHEMICAL TOXICOLOGY LA English DT Article DE ToxCast; Food additive; Food contact substance; Pesticide; High-throughput screening ID NORDIHYDROGUAIARETIC ACID NDGA; ENVIRONMENTAL CHEMICALS; RISK-ASSESSMENT; BIOACTIVITY; FRAMEWORK; UPDATE AB Thousands of chemicals are directly added to or come in contact with food, many of which have undergone little to no toxicological evaluation. The landscape of the food-relevant chemical universe was evaluated using cheminformatics, and subsequently the bioactivity of food-relevant chemicals across the publicly available ToxCast highthroughput screening program was assessed. In total, 8659 food-relevant chemicals were compiled including direct food additives, food contact substances, and pesticides. Of these food-relevant chemicals, 4719 had curated structure definition files amenable to defining chemical fingerprints, which were used to cluster chemicals using a selforganizing map approach. Pesticides, and direct food additives clustered apart from one another with food contact substances generally in between, supporting that these categories not only reflect different uses but also distinct chemistries. Subsequently, 1530 food-relevant chemicals were identified in ToxCast comprising 616 direct food additives, 371 food contact substances, and 543 pesticides. Bioactivity across ToxCast was filtered for cytotoxicity to identify selective chemical effects. Initiating analyses from strictly chemical-based methodology or bioactivity/cytotoxicity-driven evaluation presents unbiased approaches for prioritizing chemicals. Although bioactivity in vitro is not necessarily predictive of adverse effects in vivo, these data provide insight into chemical properties and cellular targets through which foodrelevant chemicals elicit bioactivity. (C) 2016 The Authors. Published by Elsevier Ltd. C1 [Karmaus, Agnes L.] ILSI North Amer, Tech Comm Food & Chem Safety, Washington, DC 20005 USA. [Karmaus, Agnes L.; Filer, Dayne L.] US EPA, ORISE, Off Res & Dev, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. [Martin, Matthew T.; Houck, Keith A.] US EPA, Off Res & Dev, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. RP Karmaus, AL (reprint author), ILSI North Amer, 1156 15th St NW,Suite 200, Washington, DC 20005 USA. EM agnes.karmaus@gmail.com OI Karmaus, Agnes/0000-0003-4421-6164 FU ILSI North America Technical Committee on Food and Chemical Safety; ILSI North America Food and Chemical Safety FX The authors thank the ILSI North America Technical Committee on Food and Chemical Safety for their guidance, support, and critical review of this work. The authors would also like to thank the U.S. FDA Center for Food Safety and Applied Nutrition (CFSAN) in graciously providing CASRN for entries associated with the FDA GRAS Notices inventory, and the Flavor and Extract Manufacturers Association (FEMA) for providing CASRN of entries associated with the FEMA GRAS inventory. ALK was supported by the ILSI North America Food and Chemical Safety 2012 Summer Fellowship. NR 32 TC 6 Z9 6 U1 5 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0278-6915 EI 1873-6351 J9 FOOD CHEM TOXICOL JI Food Chem. Toxicol. PD JUN PY 2016 VL 92 BP 188 EP 196 DI 10.1016/j.fct.2016.04.012 PG 9 WC Food Science & Technology; Toxicology SC Food Science & Technology; Toxicology GA DN1CR UT WOS:000376804400019 PM 27103583 ER PT J AU Cotterill, E Hall, D Wallace, K Mundy, WR Eglen, SJ Shafer, TJ AF Cotterill, Ellese Hall, Diana Wallace, Kathleen Mundy, William R. Eglen, Stephen J. Shafer, Timothy J. TI Characterization of Early Cortical Neural Network Development in Multiwell Microelectrode Array Plates SO JOURNAL OF BIOMOLECULAR SCREENING LA English DT Article DE neurological diseases; cell-based assays; toxicology; membrane potential ID IN-VITRO; NEURONAL NETWORKS; ACTIVITY PATTERNS; RECORDINGS; CULTURES AB We examined neural network ontogeny using microelectrode array (MEA) recordings made in multiwell MEA (mwMEA) plates over the first 12 days in vitro (DIV). In primary cortical cultures, action potential spiking activity developed rapidly between DIV 5 and 12. Spiking was sporadic and unorganized at early DIV, and became progressively more organized with time, with bursting parameters, synchrony, and network bursting increasing between DIV 5 and 12. We selected 12 features to describe network activity; principal components analysis using these features demonstrated segregation of data by age at both the well and plate levels. Using random forest classifiers and support vector machines, we demonstrated that four features (coefficient of variation [CV] of within-burst interspike interval, CV of interburst interval, network spike rate, and burst rate) could predict the age of each well recording with >65% accuracy. When restricting the classification to a binary decision, accuracy improved to as high as 95%. Further, we present a novel resampling approach to determine the number of wells needed for comparing different treatments. Overall, these results demonstrate that network development on mwMEA plates is similar to development in single-well MEAs. The increased throughput of mwMEAs will facilitate screening drugs, chemicals, or disease states for effects on neurodevelopment. C1 [Cotterill, Ellese; Eglen, Stephen J.] Univ Cambridge, Dept Appl Math & Theoret Phys, Silver St, Cambridge CB3 9EW, England. [Hall, Diana; Wallace, Kathleen; Mundy, William R.; Shafer, Timothy J.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. RP Shafer, TJ (reprint author), US EPA, Integrated Syst Toxicol Div, Off Res & Dev, MD-B105-03,109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM Shafer.tim@epa.gov OI Cotterill, Ellese/0000-0003-4760-9748; Eglen, Stephen/0000-0001-8607-8025 FU Wellcome Trust; NIHR Cambridge Biomedical Research Centre studentship; Company of Biologists; [EP-13-D-000108] FX The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: E.C. was supported by a Wellcome Trust PhD studentship and NIHR Cambridge Biomedical Research Centre studentship. D.H. was supported by student services contract EP-13-D-000108 and by a traveling fellowship from the Company of Biologists. NR 35 TC 3 Z9 3 U1 4 U2 7 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1087-0571 EI 1552-454X J9 J BIOMOL SCREEN JI J. Biomol. Screen PD JUN PY 2016 VL 21 IS 5 SI SI BP 510 EP 519 DI 10.1177/1087057116640520 PG 10 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Chemistry GA DN5IB UT WOS:000377098800010 PM 27028607 ER PT J AU Solomon, HM Makris, SL Alsaid, H Bermudez, O Beyer, BK Chen, A Chen, CL Chen, Z Chmielewski, G DeLise, AM de Schaepdrijver, L Dogdas, B French, J Harrouk, W Helfgott, J Henkelman, RM Hesterman, J Hew, KW Hoberman, A Lo, CW McDougal, A Minck, DR Scott, L Stewart, J Sutherland, V Tatiparthi, AK Winkelmann, CT Wise, LD Wood, SL Ying, XY AF Solomon, Howard M. Makris, Susan L. Alsaid, Hasan Bermudez, Oscar Beyer, Bruce K. Chen, Antong Chen, Connie L. Chen, Zhou Chmielewski, Gary DeLise, Anthony M. de Schaepdrijver, Luc Dogdas, Belma French, Julian Harrouk, Wafa Helfgott, Jonathan Henkelman, R. Mark Hesterman, Jacob Hew, Kok-Wah Hoberman, Alan Lo, Cecilia W. McDougal, Andrew Minck, Daniel R. Scott, Lelia Stewart, Jane Sutherland, Vicki Tatiparthi, Arun K. Winkelmann, Christopher T. Wise, L. David Wood, Sandra L. Ying, Xiaoyou TI Micro-Cr imaging: Developing criteria for examining fetal skeletons in regulatory developmental toxicology studies - A workshop report SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Article DE Concordance criteria; Developmental toxicology; Imaging; Micro-CT; Skeletal evaluation; GLP validation ID ALIZARIN RED S; CT; RABBITS; MOUSE; RATS AB During the past two decades the use and refinements of imaging modalities have markedly increased making it possible to image embryos and fetuses used in pivotal nonclinical studies submitted to regulatory agencies. Implementing these technologies into the Good Laboratory Practice environment requires rigorous testing, validation, and documentation to ensure the reproducibility of data. A workshop on current practices and regulatory requirements was held with the goal of defining minimal criteria for the proper implementation of these technologies and subsequent submission to regulatory agencies. Micro-computed tomography (micro-CT) is especially well suited for high-throughput evaluations, and is gaining popularity to evaluate fetal skeletons to assess the potential developmental toxicity of test agents. This workshop was convened to help scientists in the developmental toxicology field understand and apply micro-CT technology to nonclinical toxicology studies and facilitate the regulatory acceptance of imaging data. Presentations and workshop discussions covered: (1) principles of micro-CT fetal imaging; (2) concordance of findings with conventional skeletal evaluations; and (3) regulatory requirements for validating the system. Establishing these requirements for micro-CT examination can provide a path forward for laboratories considering implementing this technology and provide regulatory agencies with a basis to consider the acceptability of data generated via this technology. Published by Elsevier Inc. C1 [Solomon, Howard M.; Alsaid, Hasan] GlaxoSmithKline, King Of Prussia, PA USA. [Makris, Susan L.] US EPA, Natl Ctr Environm Assessment, Washington, DC 20460 USA. [Bermudez, Oscar; Chen, Connie L.] ILSI Hlth & Environm Sci Inst, Washington, DC USA. [Beyer, Bruce K.] Sanofi US Inc, Bridgewater, NJ USA. [Chen, Antong; Dogdas, Belma] Merck Res Labs, Kenilworth, NJ USA. [Chen, Zhou; Chmielewski, Gary; Harrouk, Wafa; McDougal, Andrew; Minck, Daniel R.] US FDA, Ctr Drug Evaluat & Res, Off New Drugs, Silver Spring, MD USA. [Tatiparthi, Arun K.] Covance Labs, Greenfield, IN USA. [DeLise, Anthony M.] Nova Pharmaceut Corp, E Hanover, NJ USA. [de Schaepdrijver, Luc] Janssen R&D, Beerse, Belgium. [French, Julian] Morphol Consulting Ltd, Stoke On Trent, Staffs, England. [Helfgott, Jonathan] Stage 2 Innovat, Framingham, MI USA. [Henkelman, R. Mark] Univ Toronto, Hosp Sick Children, Toronto, ON M5G 1X8, Canada. [Hesterman, Jacob] InviCRO, Boston, MA USA. [Hew, Kok-Wah] Takeda Pharmaceut Co, Deerfield, IL USA. [Hoberman, Alan; Scott, Lelia] Charles River Labs, Preclin Serv, Horsham, PA USA. [Lo, Cecilia W.] Univ Pittsburgh, Pittsburgh, PA USA. [Stewart, Jane] ApconiX, Macclesfield, Cheshire, England. [Sutherland, Vicki] Natl Toxicol Program NIEHS, Res Triangle Pk, NC USA. [Winkelmann, Christopher T.] Pfizer Worldwide Res & Dev, La Jolla, CA USA. [Wood, Sandra L.] Merck Res Labs, Upper Gwynedd, PA USA. [Ying, Xiaoyou] Sanofi US Inc, Cambridge, MA USA. RP Makris, SL (reprint author), 1200 Penn Ave NW,Mailcode 8623P, Washington, DC 20460 USA. EM makris.susan@epa.gov FU HESI's corporate sponsors FX The authors gratefully acknowledge the contributions of the many participants and speakers who supported this workshop with their useful presentations and discussions. The workshop that forms the basis of this publication was primarily supported by in kind contributions of time, expertise, and experimental effort from public and private sector participants from the HESI DART Technical Committee. These contributions are supplemented by direct funding (that largely supports program infrastructure and management) that was provided by HESI's corporate sponsors. A list of supporting organizations (public and private) is available at www.hesiglobal.org. Workshop presentations can also be found online. NR 35 TC 0 Z9 0 U1 2 U2 3 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0273-2300 EI 1096-0295 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD JUN PY 2016 VL 77 BP 100 EP 108 DI 10.1016/j.yrtph.2016.02.018 PG 9 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA DN1FY UT WOS:000376812900012 PM 26930635 ER PT J AU Rosi-Marshall, EJ Vallis, KL Baxter, CV Davis, JM AF Rosi-Marshall, Emma J. Vallis, Kathryn L. Baxter, Colden V. Davis, John M. TI Retesting a prediction of the River Continuum Concept: autochthonous versus allochthonous resources in the diets of invertebrates SO FRESHWATER SCIENCE LA English DT Article DE gut contents; functional feeding groups; diatoms; leaves ID ORGANIC-MATTER; CARBON-SOURCES; FOOD WEBS; STREAM CONTINUUM; TROPHIC BASIS; SALMON RIVER; DYNAMICS; FLOW; MACROINVERTEBRATES; CADDISFLIES AB The River Continuum Concept (RCC) predicts that food webs (and, in particular, invertebrates) of rivers in temperate, forested drainages should exhibit a longitudinal gradient from reliance on terrestrially derived organic matter (e.g., seasonally shed leaves) in the headwaters to autochthonous sources (e.g., algae) in the mid-orders, to suspended material in larger rivers. This prediction has been evaluated by longitudinal comparisons of macroinvertebrate communities in terms of functional feeding groups (FFGs), but such an approach yields only indirect evidence regarding actual food use. To retest this prediction, we investigated invertebrate diets from the Salmon River, Idaho, by examining the gut contents of archived specimens from the longitudinal set of sites sampled in the original 1976 RCC study and by collecting invertebrates from these same sites in 2009. We detected no apparent shifts in diets over the similar to 30-y time span. The importance of allochthonous materials in invertebrate diets differed significantly among sites along the longitudinal gradient. As predicted, it was greatest at the 2nd-order headwater site (30-42% of gut contents, on average) and decreased longitudinally to 10 to 20% at the most downstream site. However, invertebrates at the 2nd-order site also consumed large percentages of autochthonous materials (35-45%), and diets contained from 35 to 75% autochthonous resources across all sites. The shredder (Yoraperla) with the highest density at the most upstream 2nd-order site had gut contents composed of 52 to 81% diatoms depending on season, illustrating the importance of autochthonous resources in the headwaters. Our findings show that measuring shifts in FFG alone without examining actual food resources present in macroinvertebrate diets may lead to erroneous inferences regarding patterns of resource use by macro invertebrates in food webs. C1 [Rosi-Marshall, Emma J.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Vallis, Kathryn L.] Loyola Univ, Dept Biol, Chicago, IL 60626 USA. [Baxter, Colden V.; Davis, John M.] Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA. [Davis, John M.] US EPA, Natl Exposure Res Lab, Athens, GA 30605 USA. RP Rosi-Marshall, EJ (reprint author), Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA.; Vallis, KL (reprint author), Loyola Univ, Dept Biol, Chicago, IL 60626 USA.; Baxter, CV; Davis, JM (reprint author), Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA.; Davis, JM (reprint author), US EPA, Natl Exposure Res Lab, Athens, GA 30605 USA. EM rosimarshalle@caryinstitute.org; kvallis15@gmail.com; baxtcold@isu.edu; davis.john@epa.gov FU Idaho NSF-EPSCoR [EPS-08-14387] FX We thank K. Donner and R. Martin for field assistance to collect macroinvertebrates. We also heartily thank G. W. Minshall for having the foresight to archive specimens that were collected in 1976 and the many people who helped collect and identify these specimens nearly 40 y ago. Funding was provided by Idaho NSF-EPSCoR (EPS-08-14387). We thank M. Berg, C. Peterson, and 2 anonymous referees for thoughtful comments on earlier versions of this manuscript. NR 35 TC 0 Z9 0 U1 35 U2 50 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2161-9549 EI 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD JUN PY 2016 VL 35 IS 2 BP 534 EP 543 DI 10.1086/686302 PG 10 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DM6OF UT WOS:000376471600007 ER PT J AU Wei, S Lee, C Wichers, L Marron, JS AF Wei, Susan Lee, Chihoon Wichers, Lindsay Marron, J. S. TI Direction-Projection-Permutation for High-Dimensional Hypothesis Tests SO JOURNAL OF COMPUTATIONAL AND GRAPHICAL STATISTICS LA English DT Article DE Distance weighted discrimination; High-dimensional hypothesis test; High-dimensional low sample size; Linear binary classification; Permutation test; Two-sample problem ID MULTIVARIATE 2-SAMPLE TEST; SETTINGS AB High-dimensional low sample size (HDLSS) data are becoming increasingly common in statistical applications. When the data can be partitioned into two classes, a basic task is to construct a classifier that can assign objects to the correct class. Binary linear classifiers have been shown to be especially useful in HDLSS settings and preferable to more complicated classifiers because of their ease of interpretability. We propose a computational tool called direction-projection-permutation (DiProPerm), which rigorously assesses whether a binary linear classifier is detecting statistically significant differences between two high-dimensional distributions. The basic idea behind DiProPerm involves working directly with the one-dimensional projections of the data induced by binary linear classifier. Theoretical properties of DiProPerm are studied under the HDLSS asymptotic regime whereby dimension diverges to infinity while sample size remains fixed. We show that certain variations of DiProPerm are consistent and that consistency is a nontrivial property of tests in the HDLSS asymptotic regime. The practical utility of DiProPerm is demonstrated on HDLSS gene expression microarray datasets. Finally, an empirical power study is conducted comparing DiProPerm to several alternative two-sample HDLSS tests to understand the advantages and disadvantages of each method. C1 [Wei, Susan; Lee, Chihoon; Marron, J. S.] Univ N Carolina, Dept Stat & Operat Res, Chapel Hill, NC 27599 USA. [Lee, Chihoon] Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA. [Wichers, Lindsay] Univ N Carolina, Sch Publ Hlth, Dept Environm Sci & Engn, Chapel Hill, NC 27599 USA. [Wichers, Lindsay] US EPA, Environm Media Assessment Grp, Natl Ctr Environm Assessment, Off Res & Dev, MD B243-01, Res Triangle Pk, NC 27711 USA. RP Wei, S; Lee, C; Marron, JS (reprint author), Univ N Carolina, Dept Stat & Operat Res, Chapel Hill, NC 27599 USA.; Lee, C (reprint author), Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA.; Wichers, L (reprint author), Univ N Carolina, Sch Publ Hlth, Dept Environm Sci & Engn, Chapel Hill, NC 27599 USA.; Wichers, L (reprint author), US EPA, Environm Media Assessment Grp, Natl Ctr Environm Assessment, Off Res & Dev, MD B243-01, Res Triangle Pk, NC 27711 USA. EM susanwe@live.unc.edu; chihoon@stat.colostate.edu; wichers.lindsay@epa.gov; marron@email.unc.edu FU NSF; NIH [T32 GM067553-05S1] FX The work presented in this article was supported in part by the NSF Graduate Fellowship and NIH grant T32 GM067553-05S1. NR 25 TC 3 Z9 3 U1 0 U2 4 PU AMER STATISTICAL ASSOC PI ALEXANDRIA PA 732 N WASHINGTON ST, ALEXANDRIA, VA 22314-1943 USA SN 1061-8600 EI 1537-2715 J9 J COMPUT GRAPH STAT JI J. Comput. Graph. Stat. PD JUN PY 2016 VL 25 IS 2 BP 549 EP 569 DI 10.1080/10618600.2015.1027773 PG 21 WC Statistics & Probability SC Mathematics GA DM7PF UT WOS:000376551800012 ER PT J AU Novak, JM Ippolito, JA Lentz, RD Spokas, KA Bolster, CH Sistani, K Trippe, KM Phillips, CL Johnson, MG AF Novak, J. M. Ippolito, J. A. Lentz, R. D. Spokas, K. A. Bolster, C. H. Sistani, K. Trippe, K. M. Phillips, C. L. Johnson, M. G. TI Soil Health, Crop Productivity, Microbial Transport, and Mine Spoil Response to Biochars SO BIOENERGY RESEARCH LA English DT Article DE Biochar; Microbiology; Mine-impacted spoils; Restoration; Soil health ID BLACK CARBON BIOCHAR; HYDRAULIC-PROPERTIES; WATER AVAILABILITY; ESCHERICHIA-COLI; CONTAMINATED SOILS; CALCAREOUS SOIL; SANDY SOIL; PYROLYSIS; REMEDIATION; AMENDMENTS AB Biochars vary widely in pH, surface area, nutrient concentration, porosity, and metal binding capacity due to the assortment of feedstock materials and thermal conversion conditions under which it is formed. The wide variety of chemical and physical characteristics have resulted in biochar being used as an amendment to rebuild soil health, improve crop yields, increase soil water storage, and restore soils/spoils impacted by mining. Meta-analysis of the biochar literature has shown mixed results when using biochar as a soil amendment to improve crop productivity. For example, in one meta-analysis, biochar increased crop yield by approximately 10 %, while in another, approximately 50 % of the studies reported minimal to no crop yield increases. In spite of the mixed crop yield reports, biochars have properties that can improve soil health characteristics, by increasing carbon (C) sequestration and nutrient and water retention. Biochars also have the ability to bind enteric microbes and enhance metal binding in soils impacted by mining. In this review, we present examples of both effective and ineffective uses of biochar to improve soil health for agricultural functions and reclamation of degraded mine spoils. Biochars are expensive to manufacture and cannot be purged from soil after application, so for efficient use, they should be targeted for specific uses in agricultural and environmental sectors. Thus, we introduce the designer biochar concept as an alternate paradigm stating that biochars should be designed with properties that are tailored to specific soil deficiencies or problems. We then demonstrate how careful selection of biochars can increase their effectiveness as a soil amendment. C1 [Novak, J. M.] USDA ARS, Coastal Plain Res Ctr, Florence, SC 29501 USA. [Ippolito, J. A.; Lentz, R. D.] USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Spokas, K. A.] USDA ARS, Soil & Water Management Res Unit, St Paul, MN 55108 USA. [Bolster, C. H.; Sistani, K.] USDA ARS, Food Anim Environm Syst Res Unit, Bowling Green, KY 42101 USA. [Trippe, K. M.; Phillips, C. L.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Johnson, M. G.] US EPA, Western Ecol Div, Natl Hlth & Environm Effects Res Lab, Corvallis, OR 97333 USA. RP Novak, JM (reprint author), USDA ARS, Coastal Plain Res Ctr, Florence, SC 29501 USA. EM jeff.novak@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service [60-6657-1-024]; US EPA [DE-12-92342301-1] FX Sincere gratitude is expressed to the support staff at involved ARS and United States Environmental Protection Agency (US EPA) locations whose hard work made this research article possible. Parts of the information in this article have been funded through an Interagency Agreement between the United States Department of Agriculture-Agricultural Research Service (60-6657-1-024) and the US EPA (DE-12-92342301-1). It has been subject to review by scientists of the United States Department of Agriculture-Agricultural Research Service (USDA-ARS) at multiple locations and by the National Health and Environment Effects Research Laboratory's Western Ecology Division and approved for journal submission. Approval does not signify that the contents reflect the views of the USDA-ARS or the US EPA, nor does mention of trade names or commercial products constitute endorsement or recommendation for their use. NR 84 TC 3 Z9 3 U1 30 U2 52 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 454 EP 464 DI 10.1007/s12155-016-9720-8 PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600007 ER PT J AU Coffin, AW Strickland, TC Anderson, WF Lamb, MC Lowrance, RR Smith, CM AF Coffin, Alisa W. Strickland, Timothy C. Anderson, William F. Lamb, Marshall C. Lowrance, Richard R. Smith, Coby M. TI Potential for Production of Perennial Biofuel Feedstocks in Conservation Buffers on the Coastal Plain of Georgia, USA SO BIOENERGY RESEARCH LA English DT Article DE Biofuel; Bioenergy feedstocks; Landscape analysis; Miscanthus x giganteus; Pennisetum purpureum; Panicum virgatum; Georgia Coastal Plain ID SWITCHGRASS CULTIVARS; BIOENERGY FEEDSTOCK; BIOMASS PRODUCTION; GRASSES; GROWTH AB With global increases in the production of cellulosic biomass for fuel, or "biofuel," concerns over potential negative effects of using land for biofuel production have promoted attention to concepts of agricultural landscape design that sustainably balance tradeoffs between food, fuel, fiber, and conservation. The Energy Independence Security Act (EISA) of 2007 mandates an increase in advanced biofuels to 21 billion gallons in 2022. The southeastern region of the USA has been identified as a contributor to meeting half of this goal. We used a GIS-based approach to estimate the production and N-removal potential of three perennial biofeedstocks planted as conservation buffers (field borders associated with riparian buffers, and grassed waterways) on the Coastal Plain of Georgia, USA. Land cover, hydrology, elevation, and soils data were used to identify locations within agricultural landscapes that are most susceptible to runoff, erosion, and nutrient loss. We estimated potential annual biomass production from these areas to be: 2.5-3.5 Tg for giant miscanthus (Miscanthus x giganteus), 2-8.6 Tg for "Merkeron" napier grass (Pennisetum purpureum), and 1.9-7.5 Tg for "Alamo" switchgrass (Panicum virgatum). When production strategies were taken into consideration, we estimated total biomass yield of perennial grasses for the Georgia Coastal Plain at 2.2-9.4 Tg year(-1). Using published rates of N removal and ethanol conversion, we calculated the amount of potential N removal by these systems as 8100-51,000 Mg year(-1) and ethanol fuel production as 778-3296 Ml year(-1) (206 to 871 million gal. US). C1 [Coffin, Alisa W.; Strickland, Timothy C.; Smith, Coby M.] USDA ARS, SE Watershed Res Lab, 2316 Rainwater Rd, Tifton, GA 31793 USA. [Anderson, William F.] USDA ARS, Crop Genet & Breeding Res, 115 Coastal Way, Tifton, GA 31794 USA. [Lamb, Marshall C.] USDA ARS, Natl Peanut Res Lab, 1011 Forrester Dr,SE, Dawson, GA 39842 USA. [Lowrance, Richard R.] US EPA, Natl Risk Management Res Lab, 919 Kerr Res Rd, Ada, OK 74820 USA. RP Coffin, AW (reprint author), USDA ARS, SE Watershed Res Lab, 2316 Rainwater Rd, Tifton, GA 31793 USA. EM alisa.coffin@ars.usda.gov OI Coffin, Alisa/0000-0003-1608-1776 NR 50 TC 0 Z9 0 U1 4 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 587 EP 600 DI 10.1007/s12155-015-9700-4 PG 14 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600017 ER PT J AU Chen, YC Shen, GF Su, S Du, W Huangfu, YB Liu, GQ Wang, XL Xing, BS Smith, KR Tao, S AF Chen, Yuanchen Shen, Guofeng Su, Shu Du, Wei Huangfu, Yibo Liu, Guangqing Wang, Xilong Xing, Baoshan Smith, Kirk R. Tao, Shu TI Efficiencies and pollutant emissions from forced-draft biomass-pellet semi-gasifier stoves: Comparison of International and Chinese water boiling test protocols SO ENERGY FOR SUSTAINABLE DEVELOPMENT LA English DT Article DE Pellet-gasifier stoves; Water boiling test; Emission factors; Emission rate; Indoor air quality guidelines ID POLYCYCLIC AROMATIC-HYDROCARBONS; CARBONACEOUS PARTICULATE MATTER; RESIDENTIAL WOOD COMBUSTION; ENERGY EFFICIENCY; HOUSEHOLD STOVES; GREENHOUSE GASES; RURAL HOUSEHOLDS; COOKSTOVES; COOKING; PARENT AB Biomass fuels are widely combusted in rural China, producing numerous air pollutants with great adverse impacts on human health. Some improved cookstoves and pellet fuels have been promoted. To evaluate the performance of pellet-gasifier stoves, efficiencies and pollutant emissions were measured following International and Chinese water boiling tests (WBTs). Compared with traditional stoves and unprocessed biomass fuels, increased efficiencies and lower emissions of pollutants including carbon monoxide (CO), particulate matter (PM), parent and derivative polycyclic aromatic hydrocarbons (PAHs) were revealed for pellet-gasifier stoves. However, the calculated emission rates (ERs) of CO and PM2.5 cannot meet the ER targets recently suggested by WHO indoor air quality guidelines (IAQGs). Better control of air mixing ratio and gross flow rates of primary and secondary air supply greatly reduced emissions and increased efficiencies. Differences among testing protocols are the key factors affecting the evaluation of stove performance. With longer burning duration and higher power, the Chinese WBT had statistically higher efficiencies, gas temperature, and lower pollutant emissions (p < 0.10) compared to those obtained through the International WBT. Statistically significant differences between the two protocols indicate the need for further efforts in emission tests and methodology development before the release of a well-accepted international testing protocol. (C) 2016 International Energy Initiative. Published by Elsevier Inc. All rights reserved. C1 [Chen, Yuanchen; Shen, Guofeng; Su, Shu; Du, Wei; Wang, Xilong; Tao, Shu] Peking Univ, Coll Urban & Environm Sci, Lab Earth Surface Proc, Beijing 100871, Peoples R China. [Huangfu, Yibo; Liu, Guangqing] Beijing Univ Chem Technol, Biomass Energy & Environm Engn Res Ctr, Beijing 100029, Peoples R China. [Xing, Baoshan] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA. [Smith, Kirk R.] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA. [Shen, Guofeng] US EPA, NRMRL, Durham, NC 27710 USA. RP Tao, S (reprint author), Peking Univ, Coll Urban & Environm Sci, Lab Earth Surface Proc, Beijing 100871, Peoples R China. EM taos@pku.edu.cn OI Tao, Shu/0000-0002-7374-7063 FU National Natural Science Foundation of China [41390240, 41130754, 41328003, 41301554] FX Funding for this study was provided by the National Natural Science Foundation of China (41390240, 41130754, 41328003, and 41301554). NR 45 TC 0 Z9 0 U1 7 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0973-0826 J9 ENERGY SUSTAIN DEV JI Energy Sustain Dev. PD JUN PY 2016 VL 32 BP 22 EP 30 DI 10.1016/j.esd.2016.02.008 PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA DL8AF UT WOS:000375861000003 ER PT J AU Gutsch, M Hoffman, J AF Gutsch, Michelle Hoffman, Joel TI A review of Ruffe (Gymnocephalus cernua) life history in its native versus non-native range SO REVIEWS IN FISH BIOLOGY AND FISHERIES LA English DT Review DE Ruffe; Life history; Adaptability; Invasive species; Life cycle; Tolerances; Thresholds ID PERCH PERCA-FLUVIATILIS; ST-LOUIS RIVER; PIKE ESOX-LUCIUS; PULP-MILL EFFLUENT; FRESH-WATER FISH; LAKE-SUPERIOR; GREAT-LAKES; EURASIAN RUFFE; LATERAL-LINE; BALTIC SEA AB Invasive Ruffe (Gymnocephalus cernua) has caused substantial ecological damage in North America, parts of Western Europe, Scandinavian countries, and the United Kingdom. The objectives of this review are to define Ruffe's native and non-native range, examine life history requirements, explore the life cycle, and differentiate between life stages. We compare data from its native and non-native ranges to determine if there are any differences in habitat, size, age, genotype, or seasonal migration. Literature from both the native and non-native ranges of Ruffe, with some rare, translated literature, is used. In each life stage, Ruffe exhibit plasticity with regard to chemical, physical, biological, and habitat requirements. Adult Ruffe has characteristics that allow them to adapt to a range of environments, including rapid maturation, relatively long life and large size (allowing them to reproduce many times in large batches), batch spawning, genotype and phenotype (having plasticity in their genetic expression), tolerance to a wide range of water quality, broad diet, and multiple dispersal periods. There is, however, variability among these characteristics between the native, non-native North American, and European non-native populations, which presents a challenge to managing populations based on life history characteristics. Monitoring and preventative strategies are important because, based on Ruffe's variable life history strategies and its recent range expansion, all of the Laurentian Great Lakes and many other water bodies in the UK, Europe, and Norway are vulnerable to Ruffe establishment. C1 [Gutsch, Michelle] Univ Minnesota Twin Cities, UMD Biol, 207 SSB,D170A,1035 Kirby Dr, Duluth, MN 55812 USA. [Hoffman, Joel] US EPA, Off Res & Dev, Mid Continent Ecol Div, Natl Hlth & Environm Effects Res Lab, 6201 Congdon Blvd, Duluth, MN 55804 USA. RP Gutsch, M (reprint author), Univ Minnesota Twin Cities, UMD Biol, 207 SSB,D170A,1035 Kirby Dr, Duluth, MN 55812 USA. EM guts0007@umn.edu; Hoffman.Joel@epa.gov FU US EPA; University of Minnesota FX We thank the US Fish and Wildlife Service of Ashland, WI, the US Geological Survey, and the US Environmental Protection Agency (EPA) for providing Ruffe survey data, making it possible to construct Ruffe distribution maps in the US. We especially thank Derek Ogle for providing us with his library of Ruffe literature that was extremely helpful to writing this manuscript. We thank the US EPA for providing funding for this project through a cooperative agreement with the University of Minnesota. We thank the many people who helped review this paper, especially Charles Gornik and Emily Heald, who reviewed it many times. We also thank anonymous reviewers, Carol Stepien, and Ian Winfield for providing data and comments to the manuscript. The views expressed in this paper are those of the authors and do not necessarily reflect the views or policies of the US EPA. NR 151 TC 0 Z9 0 U1 15 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0960-3166 EI 1573-5184 J9 REV FISH BIOL FISHER JI Rev. Fish. Biol. Fish. PD JUN PY 2016 VL 26 IS 2 BP 213 EP 233 DI 10.1007/s11160-016-9422-5 PG 21 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DM3UW UT WOS:000376273200006 ER PT J AU Krueger, WS Hilborn, ED Dufour, AP Sams, EA Wade, TJ AF Krueger, W. S. Hilborn, E. D. Dufour, A. P. Sams, E. A. Wade, T. J. TI Self-Reported Acute Health Effects and Exposure to Companion Animals SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Pets; acute disease; signs and symptoms; zoonotic diseases; allergy ID RECREATIONAL WATER-QUALITY; RAPIDLY MEASURED INDICATORS; PUBLIC-HEALTH; PET OWNERSHIP; UNITED-STATES; ILLNESS; INFECTIONS; CONTACT; MANAGEMENT; ALLERGIES AB To understand the etiological burden of disease associated with acute health symptoms [e.g. gastrointestinal (GI), respiratory, dermatological], it is important to understand how common exposures influence these symptoms. Exposures to familiar and unfamiliar animals can result in a variety of health symptoms related to infection, irritation and allergy; however, few studies have examined this association in a large-scale cohort setting. Cross-sectional data collected from 50507 participants in the United States enrolled from 2003 to 2009 were used to examine associations between animal contact and acute health symptoms during a 10-12day period. Fixed-effects multivariable logistic regression estimated adjusted odds ratios (AORs) and 95% confident intervals (CI) for associations between animal exposures and outcomes of GI illness, respiratory illness and skin/eye symptoms. Two-thirds of the study population (63.2%) reported direct contact with animals, of which 7.7% had contact with at least one unfamiliar animal. Participants exposed to unfamiliar animals had significantly higher odds of self-reporting all three acute health symptoms, when compared to non-animal-exposed participants (GI: AOR=1.4, CI=1.2-1.7; respiratory: AOR=1.5, CI=1.2-1.8; and skin/eye: AOR=1.9, CI=1.6-2.3), as well as when compared to participants who only had contact with familiar animals. Specific contact with dogs, cats or pet birds was also significantly associated with at least one acute health symptom; AORs ranged from 1.1 to 1.5, when compared to participants not exposed to each animal. These results indicate that contact with animals, especially unfamiliar animals, was significantly associated with GI, respiratory and skin/eye symptoms. Such associations could be attributable to zoonotic infections and allergic reactions. Etiological models for acute health symptoms should consider contact with companion animals, particularly exposure to unfamiliar animals. Prevention of pet-associated zoonotic diseases includes commonsense measures such as hand-washing, but are often overlooked by pet owners and non-pet owners alike. C1 [Krueger, W. S.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Krueger, W. S.] RTI Hlth Solut, Res Triangle Pk, NC USA. [Hilborn, E. D.; Sams, E. A.; Wade, T. J.] US EPA, Off Res & Dev, Natl Hlth Effects & Environm Effects Res Lab, Chapel Hill, NC 27514 USA. [Dufour, A. P.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Cincinnati, OH USA. RP Wade, TJ (reprint author), US EPA, 104 Mason Farm Rd, Chapel Hill, NC 27514 USA. EM wade.tim@epa.gov FU Internship/Research Participation Program at the Office of Research and Development, U.S. EPA FX The manuscript has been subjected to the U.S. Environmental Protection Agency's peer review and has been approved for publication. We thank Dr. Donna Hill of the U.S. EPA for her review and comments. The views expressed in this paper are those of the authors and do not necessarily reflect the views or policies of the U.S. EPA. This project was supported in part by an appointment to the Internship/Research Participation Program at the Office of Research and Development, U.S. EPA, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. NR 37 TC 0 Z9 0 U1 5 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD JUN PY 2016 VL 63 IS 4 BP 311 EP 319 DI 10.1111/zph.12233 PG 9 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA DM3TJ UT WOS:000376269300007 PM 26514953 ER PT J AU Beck, R Zhan, SG Liu, HX Tong, S Yang, B Xu, M Ye, ZX Huang, Y Shu, S Wu, QS Wang, SJ Berling, K Murray, A Emery, E Reif, M Harwood, J Young, J Nietch, C Macke, D Martin, M Stillings, G Stump, R Su, HB AF Beck, Richard Zhan, Shengan Liu, Hongxing Tong, Susanna Yang, Bo Xu, Min Ye, Zhaoxia Huang, Yan Shu, Song Wu, Qiusheng Wang, Shujie Berling, Kevin Murray, Andrew Emery, Erich Reif, Molly Harwood, Joseph Young, Jade Nietch, Christopher Macke, Dana Martin, Mark Stillings, Garrett Stump, Richard Su, Haibin TI Comparison of satellite reflectance algorithms for estimating chlorophyll-a in a temperate reservoir using coincident hyperspectral aircraft imagery and dense coincident surface observations SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Chlorophyll-a; Algal bloom; Harmful algal bloom; Algorithm; Satellite; Hyperspectral; Multispectral ID TURBID PRODUCTIVE WATERS; REMOTE-SENSING METHODS; THEMATIC MAPPER DATA; SPECTRAL REFLECTANCE; ALGAL CHLOROPHYLL; INLAND WATERS; OCEAN COLOR; 700 NM; QUALITY; LAKE AB We compared 10 established and 2 new satellite reflectance algorithms for estimating chlorophyll-a (Chl-a) in a temperate reservoir in southwest Ohio using coincident hyperspectral aircraft imagery and dense coincident surface observations collected within 1 h of image acquisition to develop simple proxies for algal blooms in water bodies sensitive to algal blooms (especially toxic or harmful algal blooms (HABs)) and to facilitate portability between multispectral satellite imagers for regional algal bloom monitoring. All algorithms were compared with narrow band hyperspectral aircraft images. These images were subsequently upscaled spectrally and spatially to simulate 5 current and near future satellite imaging systems. Established and new Chl-a algorithms were then applied to the synthetic satellite images and compared to coincident surface observations of Chl-a collected from 44 sites within 1 h of aircraft acquisition of the imagery. We found several promising algorithm/satellite imager combinations for routine Chl-a estimation in smaller inland water bodies with operational and near-future satellite systems. The CI, MCI, FLH, NDCI, 2BDA and 3 BDA Chl-a algorithms worked well with CASI imagery. The NDCI, 2BDA, and 3BDA Chl-a algorithms worked well with simulated WorldView-2 and 3, Sentinel-2, and MERIS-like imagery. NDCI was the most widely applicable Chl-a algorithm with good performance for CASI, WorldView 2 and 3, Sentinel-2 and MERIS-like imagery and limited performance with MODIS imagery. A new fluorescence line height "greenness" algorithm yielded the best Chl-a estimates with simulated Landsat-8 imagery. (C) 2016 Elsevier Inc. All rights reserved. C1 [Beck, Richard; Zhan, Shengan; Liu, Hongxing; Tong, Susanna; Yang, Bo; Xu, Min; Ye, Zhaoxia; Huang, Yan; Shu, Song; Wu, Qiusheng; Wang, Shujie; Berling, Kevin; Murray, Andrew] Univ Cincinnati, Dept Geog, Cincinnati, OH 45221 USA. [Emery, Erich] US Army, Corps Engineers, Great Lakes & Ohio River Div, Cincinnati, OH 45202 USA. [Reif, Molly; Harwood, Joseph] US Army, Corps Engineers, Erdc, JALBTCX, Kiln, MS 39556 USA. [Young, Jade] US Army, Corps Engineers, Water Qual, Louisville, KY 40202 USA. [Nietch, Christopher; Macke, Dana] US EPA, Cincinnati, OH 45268 USA. [Martin, Mark; Stillings, Garrett; Stump, Richard] Kentucky Dept Environm Protect, Div Water, Frankfort, KY 40601 USA. NOAA, Natl Ocean Serv, Silver Spring, MD USA. [Su, Haibin] Texas A&M Kingsville, Dept Phys & Geosci, Kingsville, TX 78363 USA. RP Beck, R (reprint author), Univ Cincinnati, Dept Geog, Cincinnati, OH 45221 USA. EM richard.beck@uc.edu; zhansn@mail.uc.edu; hongxing.liu@uc.edu; susana.tong@uc.edu; yangb2@mail.uc.edu; xum4@mail.uc.edu; yeza@ucmail.uc.edu; huang2y2@ucmail.uc.edu; shusg@mail.uc.edu; wuqe@mail.uc.edu; wang2sj@mail.uc.edu; berlinkj@mail.uc.edu; murraya2@mail.uc.edu; erich.b.emery@usace.army.mil; molly.k.reif@usace.army.mil; joseph.h.harwood@usace.army.mil; jade.l.young@usace.army.mil; nietch.christopher@epa.gov; macke.dana@epa.gov; mark.martin@ky.gov; garrett.stillings@ky.gov; richard.stumpf@noaa.gov; haibin.su@tamuk.edu OI Su, Haibin/0000-0002-3645-2047; Zhan, Shengan/0000-0002-9303-1249; Wu, Qiusheng/0000-0001-5437-4073; Tong, Susanna /0000-0003-0308-5890 FU U.S. Army Corps of Engineers FX This study was funded by the U.S. Army Corps of Engineers. The U.S. Environmental Protection Agency and the Kentucky Department of Environmental Protection, Division of Water provided valuable in-kind services. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. This article expresses only the personal views of the U.S. Army Corps of Engineers employees listed as authors and does not necessarily reflect the official positions of the Corps or of the Department of the Army. NR 78 TC 0 Z9 0 U1 13 U2 24 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUN 1 PY 2016 VL 178 BP 15 EP 30 DI 10.1016/j.rse.2016.03.002 PG 16 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DL3BC UT WOS:000375508200002 ER PT J AU Wang, P Linker, LC Shenk, GW AF Wang, Ping Linker, Lewis C. Shenk, Gary W. TI Using Geographically Isolated Loading Scenarios to Analyze Nitrogen and Phosphorus Exchanges and Explore Tailored Nutrient Control Strategies for Efficient Management SO ENVIRONMENTAL MODELING & ASSESSMENT LA English DT Article DE Nutrient exchange; Nitrogen; Phosphorus; Dissolved oxygen; Geo-loading model ID CHESAPEAKE BAY; LIMITATION; PHYTOPLANKTON; CULTURES; ESTUARY; GROWTH; MODEL AB A set of geographically isolated differential nitrogen (N) and phosphorus (P) load model scenarios from major Chesapeake basins provides information on the relative impact of nutrient loads on primary production and dissolved oxygen in the Chesapeake Bay. Model results show the relationships of deep water dissolved oxygen with nutrient limitation-related algal blooms, organic carbon loads from the watershed, estuarine circulation, nutrient cycling, and nutrient diagenesis. The combined effect of changes in load from multiple basins is additive for changes in both chlorophyll-a and deep water dissolved oxygen concentrations. Management of both N and P are required in the Chesapeake watershed and tidal waters to achieve water quality standards, but overall efficiencies could be gained with strategies that place greater emphasis on P control in the upper Bay and greater emphasis on N control in the lower Bay. The areas of the Bay with the highest degree of dissolved oxygen degradation that generally drive management decisions are mostly P-limited and are significantly influenced by the load from the upper Bay's basins. Reducing P from the upper Bay's basins will intensify P limitation and would allow an increase in N of about six times the weight of P reduction. Combining the relative nutrient reduction effectiveness with the relative control cost information could improve management efficiency and provide benefits at a lower cost. This article describes initial steps that can be taken to examine the benefits from N-P exchanges. C1 [Wang, Ping] Virginia Inst Marine Sci, Chesapeake Bay Off, 410 Severn Ave, Annapolis, MD 21403 USA. [Linker, Lewis C.; Shenk, Gary W.] US EPA, Chesapeake Bay Program, Annapolis, MD USA. RP Wang, P (reprint author), Virginia Inst Marine Sci, Chesapeake Bay Off, 410 Severn Ave, Annapolis, MD 21403 USA. EM pwang@chesapeakebay.net NR 37 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1420-2026 EI 1573-2967 J9 ENVIRON MODEL ASSESS JI Environ. Model. Assess. PD JUN PY 2016 VL 21 IS 3 BP 437 EP 454 DI 10.1007/s10666-015-9487-x PG 18 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK4TO UT WOS:000374912700009 ER PT J AU Maynard, JJ Johnson, MG AF Maynard, Jonathan J. Johnson, Mark G. TI Uncoupling the complexity of forest soil variation: Influence of terrain indices, spectral indices, and spatial variability SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest soil; Remote sensing; Terrain analysis; Spatial variability; Redundancy analysis; Variation partitioning ID VEGETATION WATER-CONTENT; DIGITAL ELEVATION MODEL; LEAF-AREA INDEX; ETM PLUS DATA; ENVIRONMENTAL CORRELATION; LANDSCAPE; REFLECTANCE; ATTRIBUTES; PREDICTION; DEM AB Growing concern over climate and management induced changes to soil nutrient status has prompted interest in understanding the spatial distribution of forest soil properties. Recent advancements in remotely sensed geospatial technologies are providing an increasing array of data sources (e.g., LiDAR, hyper spectral imagery) relating to forest biophysical properties. While these data sources have the potential to improve spatial predictions of forest soil properties, considerable uncertainty exists regarding which remotely sensed (RS) indices are correlated to soil variability and what underlying pedogenic processes connect them. The main objective of this study was to identify and interpret RS indices that account for soil variability within a 2300 ha forested watershed. Redundancy analysis (RDA) and variation partitioning methods were used to uncouple the complexity of soil-environmental relationships. Thirty-two soil pedons were described, sampled, characterized and analyzed for 22 soil properties within the 0-50 cm soil depth interval. A suite of environmental covariates, comprised of LiDAR derived canopy metrics, land-surface and hydrologic terrain indices, broad-band remotely sensed indices (GeoEye-1), and narrow-band hyper-spectral indices (HyMap), were used as covariates in our RDA models. Principal coordinates of neighbor matrices (PCNM) was used to disentangle the contribution of spatial autocorrelation among sampling locations to the total variance explained by our RDA models. Two groups of soil properties were identified using discriminate analysis of principal components, with each soil property group (SPG) relating to different pedogenic processes occurring with the watershed (SPG1: organic matter metal cycling; SPG2: base-cation cycling). Our results show there was a relatively strong correspondence between soil properties and terrain/spectral indices; with 61% and 81% of the total variance explained by the first four RDA axes for SPG1 and SPG2, respectively. Variation partitioning analysis revealed that both SPG1 and SPG2 were most strongly related to terrain and canopy indices; although spectral indices were also important, especially for SGP2. Variation in the types of RS indices correlated to each SPG results from variation in the degree to which each environmental covariate relates to the pedogenic process (es) driving soil property development. The approach used in this study can help improve our understanding of soil spatial variability through identifying the most significant environmental covariates related to soil variation. Given the growing demands placed upon forest ecosystems (e.g., timber, recreation, carbon sequestration), improved knowledge of soil variability and the factors that affect the soil resource is essential to facilitate more effective forest management. Published by Elsevier B.V. C1 [Maynard, Jonathan J.] New Mexico State Univ, USDA ARS, Jornada Expt Range, POB 30003,MSC 3JER, Las Cruces, NM 88003 USA. [Johnson, Mark G.] US EPA, Natl Hlth & Environm Effects Res Lab, Western Ecol Div, 200 SW 35th St, Corvallis, OR USA. RP Maynard, JJ (reprint author), New Mexico State Univ, USDA ARS, Jornada Expt Range, POB 30003,MSC 3JER, Las Cruces, NM 88003 USA. EM jmaynard@nmsu.edu; johnson.markg@epa.gov NR 100 TC 0 Z9 0 U1 9 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 1 PY 2016 VL 369 BP 89 EP 101 DI 10.1016/j.foreco.2016.03.018 PG 13 WC Forestry SC Forestry GA DK8ID UT WOS:000375169500010 ER PT J AU Pandya, JD Royland, JE MacPhail, RC Sullivan, PG Kodavanti, PRS AF Pandya, Jignesh D. Royland, Joyce E. MacPhail, Robert C. Sullivan, Patrick G. Kodavanti, Prasada Rao S. TI Age- and brain region-specific differences in mitochondrial bioenergetics in Brown Norway rats SO NEUROBIOLOGY OF AGING LA English DT Article DE Aging; Bioenergetics; Enzyme activity; Mitochondria; Brain regions; Rats; Susceptibility ID SPINAL-CORD-INJURY; OXIDATIVE STRESS; ALZHEIMERS-DISEASE; NEURODEGENERATIVE DISEASES; YOUNG-ADULT; MIDDLE-AGE; LIFE-SPAN; ENZYMATIC-ACTIVITIES; ENERGY-METABOLISM; GENE-EXPRESSION AB Mitochondria are central regulators of energy homeostasis and play a pivotal role in mechanisms of cellular senescence. The objective of the present study was to evaluate mitochondrial bioenergetic parameters in 5 brain regions (brain stem [BS], frontal cortex, cerebellum, striatum, hippocampus [HIP]) of 4 diverse age groups (1 month [young], 4 months [adult], 12 months [middle-aged], 24 months [old age]) to understand age-related differences in selected brain regions and their possible contribution to age-related chemical sensitivity. Mitochondrial bioenergetic parameters and enzyme activities were measured under identical conditions across multiple age groups and brain regions in Brown Norway rats (n = 5/group). The results indicate age-and brain regionespecific patterns in mitochondrial functional endpoints. For example, an age-specific decline in ATP synthesis (State III respiration) was observed in BS and HIP. Similarly, the maximal respiratory capacities (State V-1 and V-2) showed age-specific declines in all brain regions examined (young > adult > middle-aged > old age). Amongst all regions, HIP had the greatest change in mitochondrial bioenergetics, showing declines in the 4, 12, and 24-months age groups. Activities of mitochondrial pyruvate dehydrogenase complex and electron transport chain complexes I, II, and IV enzymes were also age and brain region specific. In general, changes associated with age were more pronounced with enzyme activities declining as the animals aged (young > adult > middle-aged > old age). These age-and brain region-specific observations may aid in evaluating brain bioenergetic impact on the age-related susceptibility to environmental chemical stressors. Published by Elsevier Inc. C1 [Pandya, Jignesh D.; Sullivan, Patrick G.] Univ Kentucky, Dept Anat & Neurobiol, Spinal Cord & Brain Injury Res Ctr, Lexington, KY 40536 USA. [Royland, Joyce E.] US EPA, Genet & Cellular Toxicol Branch, Integrated Syst Toxicol Div, NHEERL ORD, Res Triangle Pk, NC 27711 USA. [MacPhail, Robert C.; Kodavanti, Prasada Rao S.] US EPA, Neurotoxicol Branch, Tox Assessment Div, NHEERL ORD, B105-04, Res Triangle Pk, NC 27711 USA. RP Kodavanti, PRS (reprint author), US EPA, Neurotoxicol Branch, Tox Assessment Div, NHEERL ORD, B105-04, Res Triangle Pk, NC 27711 USA. EM kodavanti.prasada@epa.gov FU US-EPA [RFQ-RT-12-140]; NIH [NS062993, NS069633] FX This work was supported by US-EPA Contract RFQ-RT-12-140 (Jignesh D. Pandya and Patrick G. Sullivan, Co-PIs), NIH grants NS062993 (Patrick G. Sullivan) and NS069633 (Patrick G. Sullivan). The authors would like to thank Andrea Sebastian for expert technical assistance. Dr. Chris Gordon from NHEERL of USEPA, Dr. Steve Simmons from NCCT of USEPA, Research Triangle Park, NC, and Dr. Timothy Koves of Duke University, Durham, NC are acknowledged for their helpful comments on an earlier version of this manuscript. The research described in this article has been reviewed by the National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, and approved for publication. Approval does not signify that the contents necessarily reflect the views and policies of the Agency nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 79 TC 1 Z9 1 U1 0 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0197-4580 EI 1558-1497 J9 NEUROBIOL AGING JI Neurobiol. Aging PD JUN PY 2016 VL 42 BP 25 EP 34 DI 10.1016/j.neurobiolaging.2016.02.027 PG 10 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA DK7TV UT WOS:000375129500003 PM 27143418 ER PT J AU Harrill, JA Layko, D Nyska, A Hukkanen, RR Manno, RA Grassetti, A Lawson, M Martin, G Budinsky, RA Rowlands, JC Thomas, RS AF Harrill, Joshua A. Layko, Debra Nyska, Abraham Hukkanen, Renee R. Manno, Rosa Anna Grassetti, Andrea Lawson, Marie Martin, Greg Budinsky, Robert A. Rowlands, J. Craig Thomas, Russell S. TI Aryl hydrocarbon receptor knockout rats are insensitive to the pathological effects of repeated oral exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE aryl hydrocarbon receptor; liver pathology; thymic atrophy; serum chemistry; AHR knockout rat ID SPRAGUE-DAWLEY RATS; AH RECEPTOR; CHRONIC TOXICITY; DIOXIN RECEPTOR; GENE-REGULATION; T-CELLS; MICE; TCDD; DEGRADATION; EXPRESSION AB Sustained activation of the aryl hydrocarbon receptor (AHR) is believed to be the initial key event in AHR receptor-mediated tumorigenesis in the rat liver. The role of AHR in mediating pathological changes in the liver prior to tumor formation was investigated in a 4-week, repeated-dose study using adult female wild-type (WT) and AHR knockout (AHR-KO) rats treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Beginning at 8weeks of age, AHR-KO and WT rats were dosed by oral gavage with varying concentrations of TCDD (0, 3, 22, 100, 300 and 1000ngkg(-1)day(-1)). Lung, liver and thymus histopathology, hematology, serum chemistry and the distribution of TCDD in liver and adipose tissue were examined. Treatment-related increases in the severity of liver and thymus pathology were observed in WT, but not AHR-KO rats. In the liver, these included hepatocellular hypertrophy, bile duct hyperplasia, multinucleated hepatocytes and inflammatory cell foci. A loss of cellularity in the thymic cortex and thymic atrophy was observed. Treatment-related changes in serum chemistry parameters were also observed in WT, but not AHR-KO rats. Finally, dose-dependent accumulation of TCDD was observed primarily in the liver of WT rats and primarily in the adipose tissue of AHR-KO rats. The results suggest that AHR activation is the initial key event underlying the progression of histological effects leading to liver tumorigenesis following TCDD treatment. Copyright (c) 2015 John Wiley & Sons, Ltd. The role of AHR in mediating pathological changes in the liver prior to tumor formation was investigated in a 4-week, repeated-dose study using adult female wild-type (WT) and AHR knockout (AHR-KO) rats treated with varying concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD: 0, 3, 22, 100, 300 and 1000 ng kg(-1) day(-1)). Treatment-related increases in the severity of liver and thymus pathology as well as changes in serum chemistry parameters were observed in WT, but not AHR-KO rats. Dose-dependent accumulation of TCDD was observed primarily in the liver of WT rats and primarily in the adipose tissue of AHR-KO rats. C1 [Harrill, Joshua A.] Ctr Toxicol & Environm Hlth LLC, 5120 North Shore Dr, North Little Rock, AR 72118 USA. [Harrill, Joshua A.; Layko, Debra; Thomas, Russell S.] Inst Chem Safety Sci, Hamner Inst Hlth Sci, Res Triangle Pk, NC 27709 USA. [Hukkanen, Renee R.; Lawson, Marie; Martin, Greg; Budinsky, Robert A.; Rowlands, J. Craig] Dow Chem Co USA, Midland, MI 48640 USA. [Nyska, Abraham] Tel Aviv Univ, Sackler Sch Med, Consultant Toxicol Pathol, IL-36576 Timrat, Israel. [Manno, Rosa Anna; Grassetti, Andrea] SPA, Res Toxicol Ctr, Pomezia, Italy. RP Thomas, RS (reprint author), US EPA, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. EM thomas.russell@epa.gov OI Thomas, Russell/0000-0002-2340-0301 FU Dow Chemical Company FX The authors would like to thank the members of the Hamner Institutes research and support staff who assisted with this work including Carol Bobbit, Delorise Williams, Nigel Edgerton, Bethany Parks, Linda Pluta, Brianna Foley, Scott Eady, Lisa Webb and Tamera Muniz. The authors would also like to thank Dr Gabrielle Wilson and technical staff from EPL, Inc. for their valuable assistance during this study. This study was funded through a grant from The Dow Chemical Company. NR 54 TC 0 Z9 0 U1 6 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD JUN PY 2016 VL 36 IS 6 BP 802 EP 814 DI 10.1002/jat.3211 PG 13 WC Toxicology SC Toxicology GA DJ7HO UT WOS:000374382700005 PM 26278112 ER PT J AU Strickland, JD Lefew, WR Crooks, J Hall, D Ortenzio, JNR Dreher, K Shafer, TJ AF Strickland, Jenna D. Lefew, William R. Crooks, James Hall, Diana Ortenzio, Jayna N. R. Dreher, Kevin Shafer, Timothy J. TI In vitro screening of metal oxide nanoparticles for effects on neural function using cortical networks on microelectrode arrays SO NANOTOXICOLOGY LA English DT Article DE particle toxicology; nanotoxicology; Nanoparticles ID TITANIUM-DIOXIDE NANOPARTICLES; TIO2 NANOPARTICLES; ENGINEERED NANOMATERIALS; ULTRAFINE PARTICLES; NEUROTOXICITY; DOSIMETRY; EXPOSURE; TRANSLOCATION; TECHNOLOGY; RECORDINGS AB Nanoparticles (NPs) may translocate to the brain following inhalation or oral exposures, yet higher throughput methods to screen NPs for potential neurotoxicity are lacking. The present study examined effects of 5 CeO2 (5- 1288 nm), and 4 TiO2 (6-142 nm) NPs and microparticles (MP) on network function in primary cultures of rat cortex on 12 well microelectrode array (MEA) plates. Particles were without cytotoxicity at concentrations <= 50 mu g/ml. After recording 1 h of baseline activity prior to particle (3-50 mu g/ml) exposure, changes in the total number of spikes (TS) and # of active electrodes (#AEs) were assessed 1, 24, and 48 h later. Following the 48 h recording, the response to a challenge with the GABA(A) antagonist bicuculline (BIC; 25 mu M) was assessed. In all, particles effects were subtle, but 69 nm CeO2 and 25 nm TiO2 NPs caused concentration-related decreases in TS following 1 h exposure. At 48 h, 5 and 69 nm CeO2 and 25 and 31 nm TiO2 decreased #AE, while the two MPs increased #AEs. Following BIC, only 31 nm TiO2 produced concentration-related decreases in #AEs, while 1288 nm CeO2 caused concentration-related increases in both TS and #AE. The results indicate that some metal oxide particles cause subtle concentration-related changes in spontaneous and/or GABA(A) receptor-mediated neuronal activity in vitro at times when cytotoxicity is absent, and that MEAs can be used to screen and prioritize nanoparticles for neurotoxicity hazard. C1 [Strickland, Jenna D.; Lefew, William R.; Crooks, James; Hall, Diana; Ortenzio, Jayna N. R.; Dreher, Kevin; Shafer, Timothy J.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. RP Shafer, TJ (reprint author), US EPA, Integrated Syst Toxicol Div, Off Res & Dev, MD-B105-03, Res Triangle Pk, NC 27711 USA. EM Shafer.tim@epa.gov FU U.S. Environmental Protection Agency; Oak Ridge Institute for Science and Education Fellowship; [EP-11-D-000392]; [EP-13-D-000108] FX This work has been funded by the U.S. Environmental Protection Agency. This document has been reviewed by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.; Diana Hall was supported by student services contracts #EP-11-D-000392 (JDS) and #EP-13-D-000108.; Jayna NR Ortenzio was supported by Oak Ridge Institute for Science and Education Fellowship. NR 45 TC 2 Z9 2 U1 9 U2 22 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1743-5390 EI 1743-5404 J9 NANOTOXICOLOGY JI Nanotoxicology PD MAY 27 PY 2016 VL 10 IS 5 BP 619 EP 628 DI 10.3109/17435390.2015.1107142 PG 10 WC Nanoscience & Nanotechnology; Toxicology SC Science & Technology - Other Topics; Toxicology GA DH4LA UT WOS:000372756300011 PM 26593696 ER PT J AU Hudgens, EE Drobna, Z He, B Le, XC Styblo, M Rogers, J Thomas, DJ AF Hudgens, Edward E. Drobna, Zuzana He, Bin Le, X. C. Styblo, Miroslav Rogers, John Thomas, David J. TI Biological and behavioral factors modify urinary arsenic metabolic profiles in a US population SO ENVIRONMENTAL HEALTH LA English DT Article DE Inorganic arsenic; Methylated arsenicals; Drinking water; Urine; Toenails; Human; Biological and behavioral modifiers; Gender; Smoking; BMI ID OXIDATION-STATE METHYLTRANSFERASE; NUTRITION EXAMINATION SURVEY; PLASMA-MASS SPECTROMETRY; NEW-HAMPSHIRE POPULATION; BLADDER-CANCER; DRINKING-WATER; CREATININE CONCENTRATION; BANGLADESHI ADULTS; NATIONAL-HEALTH; TOBACCO-SMOKE AB Background: Because some adverse health effects associated with chronic arsenic exposure may be mediated by methylated arsenicals, interindividual variation in capacity to convert inorganic arsenic into mono- and di-methylated metabolites may be an important determinant of risk associated with exposure to this metalloid. Hence, identifying biological and behavioral factors that modify an individual's capacity to methylate inorganic arsenic could provide insights into critical dose-response relations underlying adverse health effects. Methods: A total of 904 older adults (>= 45 years old) in Churchill County, Nevada, who chronically used home tap water supplies containing up to 1850 mu g of arsenic per liter provided urine and toenail samples for determination of total and speciated arsenic levels. Effects of biological factors (gender, age, body mass index) and behavioral factors (smoking, recent fish or shellfish consumption) on patterns of arsenicals in urine were evaluated with bivariate analyses and multivariate regression models. Results: Relative contributions of inorganic, mono-, and di-methylated arsenic to total speciated arsenic in urine were unchanged over the range of concentrations of arsenic in home tap water supplies used by study participants. Gender predicted both absolute and relative amounts of arsenicals in urine. Age predicted levels of inorganic arsenic in urine and body mass index predicted relative levels of mono-and di-methylated arsenic in urine. Smoking predicted both absolute and relative levels of arsenicals in urine. Multivariate regression models were developed for both absolute and relative levels of arsenicals in urine. Concentration of arsenic in home tap water and estimated water consumption were strongly predictive of levels of arsenicals in urine as were smoking, body mass index, and gender. Relative contributions of arsenicals to urinary arsenic were not consistently predicted by concentrations of arsenic in drinking water supplies but were more consistently predicted by gender, body mass index, age, and smoking. Conclusions: These findings suggest that analyses of dose-response relations in arsenic-exposed populations should account for biological and behavioral factors that modify levels of inorganic and methylated arsenicals in urine. Evidence of significant effects of these factors on arsenic metabolism may also support mode of action studies in appropriate experimental models. C1 [Hudgens, Edward E.] US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27709 USA. [Drobna, Zuzana; Styblo, Miroslav] Univ N Carolina, Dept Nutr, Chapel Hill, NC 27599 USA. [He, Bin; Le, X. C.] Univ Alberta, Fac Med & Dent, Dept Lab Med & Pathol, Analyt & Environm Toxicol, Edmonton, AB T6G 2G3, Canada. [Rogers, John] WESTAT Corp, 1600 Res Blvd, Rockville, MD 20850 USA. [Thomas, David J.] US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27709 USA. RP Thomas, DJ (reprint author), US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27709 USA. EM thomas.david@epa.gov RI Le, X. Chris/O-4947-2015 OI Le, X. Chris/0000-0002-7690-6701 FU Churchill County Cooperative Extension (EPA) [2D-6148-NAEX]; Nevada State Health Laboratory (EPA) [2D-6285-GNGX]; Churchill Community Hospital (EPA) [D-7294-NTLX]; Westat, Inc. (EPA contract) [EP-D-07-109, 68-D-02-062, EP-D-12-050]; Westat, Inc. (GSA MOBIS contract) [GSF8144H, TO1425] FX This manuscript honors Dr. Rebecca L. Calderon (1955-2008) who organized and directed the Churchill County study. We gratefully acknowledge organizations and individuals who helped plan and conduct this field study: Guarding Our Local Drinking Water (GOLD Water) Team; University of Nevada at Reno; Churchill County Cooperative Extension (EPA contract 2D-6148-NAEX); Nevada State Health Laboratory (EPA Contract 2D-6285-GNGX); Churchill Community Hospital (EPA contract 2D-7294-NTLX); State of Nevada; Churchill County Board of Commissioners; City of Fallon, Nevada; U.S. Department of the Navy, Fallon Naval Air Station; and Westat, Inc. (EPA contracts EP-D-07-109 and 68-D-02-062, EP-D-12-050, GSA MOBIS contract GSF8144H, TO1425). We thank Zhongwen Wang for assistance with arsenic speciation analysis. This manuscript has been reviewed in accordance with the policy of the National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, and approved for publication. Approval does not signify that the contents necessarily reflect the views and policies of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 67 TC 1 Z9 1 U1 4 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1476-069X J9 ENVIRON HEALTH-GLOB JI Environ. Health PD MAY 26 PY 2016 VL 15 AR 62 DI 10.1186/s12940-016-0144-x PG 14 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DM9EE UT WOS:000376666800001 PM 27230915 ER PT J AU Li, Y Schichtel, BA Walker, JT Schwede, DB Chen, X Lehmann, CMB Puchalski, MA Gay, DA Collett, JL AF Li, Yi Schichtel, Bret A. Walker, John T. Schwede, Donna B. Chen, Xi Lehmann, Christopher M. B. Puchalski, Melissa A. Gay, David A. Collett, Jeffrey L., Jr. TI Increasing importance of deposition of reduced nitrogen in the United States SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE ammonia; dry deposition; wet deposition; nitrogen oxides; agriculture ID BIDIRECTIONAL AMMONIA EXCHANGE; MIXED CONIFEROUS FOREST; MOUNTAIN NATIONAL-PARK; REACTIVE NITROGEN; COMPENSATION POINT; MULTILAYER MODEL; DRY DEPOSITION; ATMOSPHERE EXCHANGE; PARAMETERIZATION; ENVIRONMENT AB Rapid development of agriculture and fossil fuel combustion greatly increased US reactive nitrogen emissions to the atmosphere in the second half of the 20th century, resulting in excess nitrogen deposition to natural ecosystems. Recent efforts to lower nitrogen oxides emissions have substantially decreased nitrate wet deposition. Levels of wet ammonium deposition, by contrast, have increased in many regions. Together these changes have altered the balance between oxidized and reduced nitrogen deposition. Across most of the United States, wet deposition has transitioned from being nitrate-dominated in the 1980s to ammonium-dominated in recent years. Ammonia has historically not been routinely measured because there are no specific regulatory requirements for its measurement. Recent expansion in ammonia observations, however, along with ongoing measurements of nitric acid and fine particle ammonium and nitrate, permit new insight into the balance of oxidized and reduced nitrogen in the total (wet + dry) US nitrogen deposition budget. Observations from 37 sites reveal that reduced nitrogen contributes, on average, similar to 65% of the total inorganic nitrogen deposition budget. Dry deposition of ammonia plays an especially key role in nitrogen deposition, contributing from 19% to 65% in different regions. Future progress toward reducing US nitrogen deposition will be increasingly difficult without a reduction in ammonia emissions. C1 [Li, Yi; Collett, Jeffrey L., Jr.] Colorado State Univ, Dept Atmospher Sci, Ft Collins, CO 80523 USA. [Schichtel, Bret A.] Colorado State Univ, Cooperat Inst Res Atmosphere, Natl Pk Serv, Ft Collins, CO 80523 USA. [Walker, John T.; Chen, Xi] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Schwede, Donna B.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Lehmann, Christopher M. B.; Gay, David A.] Univ Illinois, Natl Atmospher Deposit Program, Champaign, IL 61820 USA. [Puchalski, Melissa A.] US EPA, Clean Air Markets Div, Washington, DC 20460 USA. RP Collett, JL (reprint author), Colorado State Univ, Dept Atmospher Sci, Ft Collins, CO 80523 USA. EM collett@atmos.colostate.edu RI Collett, Jeffrey/F-2862-2010 OI Collett, Jeffrey/0000-0001-9180-508X FU National Park Service FX We thank Amy Sullivan, Katie Benedict, Aohan Tang, Qijing Bian, Arsineh Hecobian, and Yixing Shao (Colorado State University); Brian Michael (University of Illinois); and Nate Hyde (WaveMetrics) for helpful suggestions and support. Funding for this work was provided by the National Park Service. NR 59 TC 8 Z9 8 U1 21 U2 37 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAY 24 PY 2016 VL 113 IS 21 BP 5874 EP 5879 DI 10.1073/pnas.1525736113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN0UD UT WOS:000376779900047 PM 27162336 ER PT J AU Pauer, JJ Feist, TJ Anstead, AM DePetro, PA Melendez, W Lehrter, JC Murrell, MC Zhang, XM Ko, DS AF Pauer, James J. Feist, Timothy J. Anstead, Amy M. DePetro, Phillip A. Melendez, Wilson Lehrter, John C. Murrell, Michael C. Zhang, Xiaomi Ko, Dong S. TI A modeling study examining the impact of nutrient boundaries on primary production on the Louisiana continental shelf SO ECOLOGICAL MODELLING LA English DT Article DE Numerical model; Louisiana continental shelf (LCS); Primary production; Nutrients; Boundary concentrations; Co-limitation ID GULF-OF-MEXICO; MISSISSIPPI RIVER PLUME; PHYTOPLANKTON GROWTH; HYPOXIA; VARIABILITY; OXYGEN; SEDIMENTATION; LIMITATION; DYNAMICS; NITROGEN AB Nutrient inputs to the Louisiana continental shelf (LCS) from lateral ocean boundaries can be significant, but the effect of these nutrients on LCS primary production has not been examined. Herein, we apply a three-dimensional physical-biogeochemical model to calculate nitrogen and phosphorus mass balances on the LCS and quantify the contributions of riverine and offshore nutrient inputs to primary production. A model sensitivity analysis to different offshore nutrient concentrations indicated that modeled primary production was most sensitive to boundary nitrogen concentrations, whereas changing boundary phosphorus concentrations had little effect. The primary production response also varied spatially and temporally, with its greatest response being to changing boundary nitrogen concentrations in areas furthest from the river plume, and during the late summer for all regions of the shelf when Mississippi River discharge approaches its annual minimum. These results indicate that even for river-dominated shelves like the LCS, uncertain boundary condition nutrient concentrations are likely to contribute significantly to uncertainty in modeled primary production. The modeling study highlights the need for further observational studies to understand the sources and variability of nutrients at LCS offshore boundaries and the impacts to LCS primary production. Published by Elsevier B.V. C1 [Pauer, James J.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab,Large Lakes, Midcontinent Ecol Div,Large Lakes & Rivers Foreca, 9311 Groh Rd, Grosse Ile, MI 48138 USA. [Feist, Timothy J.; Zhang, Xiaomi] Trinity Engn Associates Inc, Large Lakes Res Stn, 9311 Groh Rd, Grosse Ile, MI 48138 USA. [Anstead, Amy M.; DePetro, Phillip A.] ICF Int, Large Lakes Res Stn, 9311 Groh Rd, Grosse Ile, MI 48138 USA. [Melendez, Wilson] CSC Govt Solut LLC, Large Lakes Res Stn, 9311 Groh Rd, Grosse Ile, MA 48138 USA. [Lehrter, John C.; Murrell, Michael C.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Gulf Ecol Div, 1 Sabine Isl Dr, Gulf Breeze, FL 32561 USA. [Ko, Dong S.] US Naval Res Lab, Stennis Space Ctr, MS 39529 USA. RP Pauer, JJ (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab,Large Lakes, Midcontinent Ecol Div,Large Lakes & Rivers Foreca, 9311 Groh Rd, Grosse Ile, MI 48138 USA. EM pauer.james@epa.gov FU US Environmental Protection Agency FX The work was funded entirely by the US Environmental Protection Agency. We thank Mark Rowe for the thorough review of the manuscript and his constructive comments, and Dave Griesmer and Ken Rygwelski for helping with the graphics. The views expressed in this paper are those of the authors and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency. NR 52 TC 1 Z9 1 U1 2 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD MAY 24 PY 2016 VL 328 BP 136 EP 147 DI 10.1016/j.ecolmodel.2016.02.007 PG 12 WC Ecology SC Environmental Sciences & Ecology GA DK2AL UT WOS:000374716900014 ER PT J AU Currier, JM Cheng, WY Menendez, D Conolly, R Chorley, BN AF Currier, Jenna M. Cheng, Wan-Yun Menendez, Daniel Conolly, Rory Chorley, Brian N. TI Developing a Gene Biomarker at the Tipping Point of Adaptive and Adverse Responses in Human Bronchial Epithelial Cells SO PLOS ONE LA English DT Article ID TOXCAST PROGRAM; IN-VITRO; ANTIOXIDANT RESPONSE; RISK-ASSESSMENT; BEAS-2B CELLS; CANCER-CELLS; ZINC; P53; TOXICITY; APOPTOSIS AB Determining mechanism-based biomarkers that distinguish adaptive and adverse cellular processes is critical to understanding the health effects of environmental exposures. Shifting from in vivo, low-throughput toxicity studies to high-throughput screening (HTS) paradigms and risk assessment based on in vitro and in silico testing requires utilizing toxicity pathway information to distinguish adverse outcomes from recoverable adaptive events. Little work has focused on oxidative stresses in human airway for the purposes of predicting adverse responses. We hypothesize that early gene expression-mediated molecular changes could be used to delineate adaptive and adverse responses to environmentally-based perturbations. Here, we examined cellular responses of the tracheobronchial airway to zinc (Zn) exposure, a model oxidant. Airway derived BEAS-2B cells exposed to 2-10 mu M Zn2+ elicited concentration-and time-dependent cytotoxicity. Normal, adaptive, and cytotoxic Zn2+ exposure conditions were determined with traditional apical endpoints, and differences in global gene expression around the tipping point of the responses were used to delineate underlying molecular mechanisms. Bioinformatic analyses of differentially expressed genes indicate early enrichment of stress signaling pathways, including those mediated by the transcription factors p53 and NRF2. After 4 h, 154 genes were differentially expressed (p < 0.01) between the adaptive and cytotoxic Zn2+ concentrations. Nearly 40% of the biomarker genes were related to the p53 signaling pathway with 30 genes identified as likely direct targets using a database of p53 ChIP-seq studies. Despite similar p53 activation profiles, these data revealed widespread dampening of p53 and NRF2-related genes as early as 4 h after exposure at higher, unrecoverable Zn2+ exposures. Thus, in our model early increased activation of stress response pathways indicated a recoverable adaptive event. Overall, this study highlights the importance of characterizing molecular mechanisms around the tipping point of adverse responses to better inform HTS paradigms. C1 [Currier, Jenna M.; Cheng, Wan-Yun] US EPA, Oak Ridge Inst Sci & Educ, Res Triangle Pk, NC 27711 USA. [Menendez, Daniel] NIEHS, Genome Integr & Struct Biol Lab, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Conolly, Rory; Chorley, Brian N.] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. RP Chorley, BN (reprint author), US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. EM chorley.brian@epa.gov FU United States Environmental Protection Agency, Office of Research and Development; Oak Ridge Institute for Science and Education Research/Participation Program at the US Environmental Protection Agency FX This work was supported by intramural funding by the United States Environmental Protection Agency, Office of Research and Development.; JMC and W-YC were supported by the Oak Ridge Institute for Science and Education Research/Participation Program at the US Environmental Protection Agency. This manuscript has been reviewed by the National Health and Environmental Effects Research Laboratory and approved for publication. The content does not necessarily reflect the views of the US EPA nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 64 TC 0 Z9 0 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 19 PY 2016 VL 11 IS 5 AR e0155875 DI 10.1371/journal.pone.0155875 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM4BR UT WOS:000376291100124 PM 27195669 ER PT J AU Vallotton, N Price, PS AF Vallotton, Nathalie Price, Paul S. TI Use of the Maximum Cumulative Ratio As an Approach for Prioritizing Aquatic Coexposure to Plant Protection Products: A Case Study of a Large Surface Water Monitoring Database SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID JOINT ALGAL TOXICITY; REALISTIC PESTICIDE MIXTURES; RISK-ASSESSMENT; CONCENTRATION ADDITION; MULTIPLE SUBSTANCES; INDEPENDENT ACTION; DECISION TREE; UNITED-STATES; CHLORPYRIFOS; CONTAMINATION AB This paper uses the maximum cumulative ratio (MCR) as part of a tiered approach to evaluate and prioritize the risk of acute ecological effects from combined exposures to the plant protection products (PPPs) measured in 3 099 surface water samples taken from across the United States. Assessments of the reported mixtures performed on a substance-by-substance approach and using a Tier One cumulative assessment based on the lowest acute ecotoxicity benchmark gave the same findings for 92.3% of the mixtures. These mixtures either did not indicate a potential risk for acute effects or included one or more individual PPPs that had concentrations in excess of their benchmarks. A Tier Two assessment using a trophic. level approach was applied to evaluate the remaining 7.7% of the mixtures. This assessment reduced the number of mixtures of concern by eliminating the combination of endpoint from multiple trophic levels, identified invertebrates and nonvascular plants as the most susceptible nontarget organisms, and indicated that a only a very limited number of PPPs drove the potential concerns. The combination of the measures of cumulative risk and the MCR enabled the identification of a small subset of mixtures where a potential risk would be missed in substance-by-substance assessments. C1 [Vallotton, Nathalie] Dow Europe GmbH, Toxicol & Environm Res & Consulting, Bachtobelstr 3, CH-8810 Horgen, Switzerland. [Price, Paul S.] Dow Chem Co USA, Toxicol & Environm Res & Consulting, 1803 Bldg, Midland, MI 48640 USA. [Price, Paul S.] US EPA, Natl Exposure Res Lab, Off Res & Dev, D305-01,109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. RP Vallotton, N (reprint author), Dow Europe GmbH, Toxicol & Environm Res & Consulting, Bachtobelstr 3, CH-8810 Horgen, Switzerland. EM navallotton@dow.com FU Dow Chemical Company FX We thank Dr. Xianglu Han for his contributions in the early stages of this analysis. The Dow Chemical Company supported this work. NR 26 TC 0 Z9 0 U1 5 U2 15 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD MAY 17 PY 2016 VL 50 IS 10 BP 5286 EP 5293 DI 10.1021/acs.est.5b06267 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DM4QJ UT WOS:000376331500045 PM 27057923 ER PT J AU Mash, H Wittkorn, A AF Mash, H. Wittkorn, A. TI Effect of chlorination on the protein phosphatase inhibition activity for several microcystins SO WATER RESEARCH LA English DT Article DE Algaltoxin; Microcystin; Protein phosphatase inhibition; Chlorination ID DRINKING-WATER; CYANOBACTERIAL TOXINS; HUMAN FATALITIES; PEPTIDE; BRAZIL; LIVER; CYANOTOXINS; ELIMINATION; EXPOSURE; KINETICS AB Microcystins are of particular concern due to their toxicity to both humans and animals and may be the most prominent cyanotoxin observed in freshwater. Although a number of studies have investigated the fate of microcystins and other algal toxins through drinking water treatment facilities, measurement of their potential for toxic activity after chlorination, a popular form of treatment in the United States, has not been investigated. In this study, six microcystin variants are subjected to chlorine oxidation. The degradation of each microcystin variant is measured by liquid chromatography/mass spectrometry simultaneously with protein phosphatase inhibition (PPI) response over reaction time with chlorine. Results show that inhibition is dependent on the incorporated amino acid residues, their placement within the microcystin structure, as well as pH. This pH dependence may have practical implications to such activities such as drinking water treatment when the pH is usually adjusted to around 8. Namely, at this pH, even with chlorine addition for disinfection, PPI activity may not be totally eliminated even when the initial MYCs are eliminated. Published by Elsevier Ltd. C1 [Mash, H.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Water Supply & Water Resources Div,Treatment Tech, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Wittkorn, A.] Univ Cincinnati, 2600 Clifton Ave, Cincinnati, OH 45220 USA. RP Mash, H (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, Water Supply & Water Resources Div,Treatment Tech, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM mash.heath@epa.gov NR 37 TC 0 Z9 0 U1 7 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD MAY 15 PY 2016 VL 95 BP 230 EP 239 DI 10.1016/j.watres.2016.03.024 PG 10 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DL7KG UT WOS:000375819000022 PM 26999255 ER PT J AU Lin, MY Hagler, G Baldauf, R Isakov, V Lin, HY Khlystov, A AF Lin, Ming-Yeng Hagler, Gayle Baldauf, Richard Isakov, Vlad Lin, Hong-Yiou Khlystov, Andrey TI The effects of vegetation barriers on near-road ultrafine particle number and carbon monoxide concentrations SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Near-road; Ultrafine particle; Carbon monoxide; Vegetation barriers; Mobile measurement ID AIR-QUALITY; PARTICULATE MATTER; URBAN-ENVIRONMENT; SUBMICROMETER; VARIABILITY; POLLUTANTS; EMISSIONS; VEHICLES; HIGHWAY; IMPACTS AB Numerous studies have shown that people living in near -roadway communities (within 100 m of the road) are exposed to high ultrafine particle (UFP) number concentrations, which may be associated with adverse health effects. Vegetation barriers have been shown to affect pollutant transport via particle deposition to leaves and altering the dispersion of emission plumes, which in turn would modify the exposure of near -roadway communities to traffic -related UlTs. In this study, both stationary (equipped with a Scanning Mobility Particle Sizer, SMPS) and mobile (equipped with Fast Mobility Particle Sizer, IMPS) measurements were conducted to investigate the effects of vegetation barriers on downwind Ulf (particle diameters ranging from 14 to 102 nm) concentrations at two sites in North Carolina, USA. One site had mainly deciduous vegetation while the other was primarily coniferous; both sites have a nearby open field without the vegetation barriers along the same stretch of limited access road, which served as a reference. During downwind conditions (traffic emissions transported towards the vegetation barrier) and when the wind speed was above or equal to 05 m/s, field measurements indicated that vegetation barriers with full foliage reduced UFP and CO concentrations by 37.7-63.6% and 23.6-56.1%, respectively. When the test was repeated at the same sites during winter periods when deciduous foliage was reduced, the deciduous barrier during winter showed no significant change in UFP concentration before and after the barrier. Results from the stationary (using SMPS) and mobile (using FMPS) measurements for UFP total number concentrations generally agreed to within 20%. 2016 Elsevier B.V. All rights reserved. C1 [Lin, Ming-Yeng] Natl Cheng Kung Univ, Coll Med, Dept Environm & Occupat Hlth, Tainan 70101, Taiwan. [Hagler, Gayle; Baldauf, Richard; Isakov, Vlad] US EPA, Off Res & Dev, New York, NY USA. [Baldauf, Richard] US EPA, Off Transportat & Air Qual, New York, NY USA. [Lin, Hong-Yiou] William Beaumont Hosp, Dept Radiat Oncol, New York, NY USA. [Khlystov, Andrey] Desert Res Inst, Div Atmospher Sci, New York, NY 10010 USA. RP Khlystov, A (reprint author), Desert Res Inst, Div Atmospher Sci, New York, NY 10010 USA. EM andrey.khlystov@dri.edu NR 40 TC 2 Z9 2 U1 17 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD MAY 15 PY 2016 VL 553 BP 372 EP 379 DI 10.1016/j.scitotenv.2016.02.035 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DI0XN UT WOS:000373220700036 PM 26930311 ER PT J AU Wegner, S Heiger-Bernays, W Dix, D AF Wegner, Susanna Heiger-Bernays, Wendy Dix, David TI Spanning regulatory silos in the US EPA's Endocrine Disruptor Screening Program Letter to the Editor re: Evans et al. "Should the scope of human mixture risk assessment span legislative and regulatory silos for chemicals?" SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Letter DE Cumulative risk; High Throughput Screening; Endocrine Disruptor Screening Program ID ESTROGEN-RECEPTOR; MODEL C1 [Wegner, Susanna] ORISE, Oak Ridge, TN USA. [Heiger-Bernays, Wendy] US EPA, AAAS, Washington, DC 20460 USA. [Dix, David] US EPA, Off Sci Coordinat & Policy, Off Chem Safety & Pollut Prevent, Washington, DC 20460 USA. RP Dix, D (reprint author), US EPA, Off Sci Coordinat & Policy, Off Chem Safety & Pollut Prevent, Washington, DC 20460 USA. EM wegner.susanna@epa.gov; dix.david@epa.gov NR 9 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD MAY 15 PY 2016 VL 553 BP 671 EP 672 DI 10.1016/j.scitotenv.2016.02.192 PG 2 WC Environmental Sciences SC Environmental Sciences & Ecology GA DI0XN UT WOS:000373220700068 PM 26972864 ER PT J AU Chaudhary, VB Rua, MA Antoninka, A Bever, JD Cannon, J Craig, A Duchicela, J Frame, A Gardes, M Gehring, C Ha, M Hart, M Hopkins, J Ji, BM Johnson, NC Kaonongbua, W Karst, J Koide, RT Lamit, LJ Meadow, J Milligan, BG Moore, JC Pendergast, TH Piculell, B Ramsby, B Simard, S Shrestha, S Umbanhowar, J Viechtbauer, W Walters, L Wilson, GWT Zee, PC Hoeksema, JD AF Chaudhary, V. Bala Rua, Megan A. Antoninka, Anita Bever, James D. Cannon, Jeffery Craig, Ashley Duchicela, Jessica Frame, Alicia Gardes, Monique Gehring, Catherine Ha, Michelle Hart, Miranda Hopkins, Jacob Ji, Baoming Johnson, Nancy Collins Kaonongbua, Wittaya Karst, Justine Koide, Roger T. Lamit, Louis J. Meadow, James Milligan, Brook G. Moore, John C. Pendergast, Thomas H. Piculell, Bridget Ramsby, Blake Simard, Suzanne Shrestha, Shubha Umbanhowar, James Viechtbauer, Wolfgang Walters, Lawrence Wilson, Gail W. T. Zee, Peter C. Hoeksema, Jason D. TI MycoDB, a global database of plant response to mycorrhizal fungi SO SCIENTIFIC DATA LA English DT Article; Data Paper ID PHYLOGENETICS; METAANALYSIS; EVOLUTION AB Plants form belowground associations with mycorrhizal fungi in one of the most common symbioses on Earth. However, few large-scale generalizations exist for the structure and function of mycorrhizal symbioses, as the nature of this relationship varies from mutualistic to parasitic and is largely contextdependent. We announce the public release of MycoDB, a database of 4,010 studies (from 438 unique publications) to aid in multi-factor meta-analyses elucidating the ecological and evolutionary context in which mycorrhizal fungi alter plant productivity. Over 10 years with nearly 80 collaborators, we compiled data on the response of plant biomass to mycorrhizal fungal inoculation, including meta-analysis metrics and 24 additional explanatory variables that describe the biotic and abiotic context of each study. We also include phylogenetic trees for all plants and fungi in the database. To our knowledge, MycoDB is the largest ecological meta-analysis database. We aim to share these data to highlight significant gaps in mycorrhizal research and encourage synthesis to explore the ecological and evolutionary generalities that govern mycorrhizal functioning in ecosystems. C1 [Chaudhary, V. Bala] Depaul Univ, Dept Environm Sci & Studies, Chicago, IL 60614 USA. [Rua, Megan A.] Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN 37996 USA. [Rua, Megan A.; Ha, Michelle; Piculell, Bridget; Ramsby, Blake; Hoeksema, Jason D.] Univ Mississippi, Dept Biol, University, MS 38677 USA. [Antoninka, Anita] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. [Bever, James D.] Univ Kansas, Dept Ecol & Evolutionary Biol, Lawrence, KS 66045 USA. [Cannon, Jeffery] Colorado State Univ, Colorado Forest Restorat Inst, Ft Collins, CO 80523 USA. [Craig, Ashley] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA. [Duchicela, Jessica; Hopkins, Jacob] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA. [Duchicela, Jessica] Univ Fuerzas Armadas ESPE, Dept Ciencias Vida, 1715231B, Sangolqui, Ecuador. [Frame, Alicia] US EPA, Off Solid Waste & Emergency Response, Washington, DC 20004 USA. [Gardes, Monique] Univ Toulouse 3, CNRS, ENFA, F-31062 Toulouse, France. [Gardes, Monique] UMR5174 EDB, F-31062 Toulouse, France. [Hart, Miranda] Univ British Columbia, Dept Biol, Kelowna, BC V1V 1V7, Canada. [Ji, Baoming] Beijing Forestry Univ, Coll Forestry, Beijing 100083, Peoples R China. [Johnson, Nancy Collins] No Arizona Univ, Sch Earth Sci & Environm Sustainabil, Flagstaff, AZ 86011 USA. [Kaonongbua, Wittaya] King Mongkuts Univ Technol Thonburi, Fac Sci, Dept Microbiol, Bangkok 10140, Thailand. [Karst, Justine] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2E3, Canada. [Koide, Roger T.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Lamit, Louis J.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Meadow, James] Univ Oregon, Inst Ecol & Evolut, Eugene, OR 97403 USA. [Meadow, James] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Milligan, Brook G.] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Moore, John C.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. [Moore, John C.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Pendergast, Thomas H.] Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA. [Simard, Suzanne] Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada. [Shrestha, Shubha] Winston Salem State Univ, Dept Biol Sci, Winston Salem, NC 27110 USA. [Umbanhowar, James] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA. [Viechtbauer, Wolfgang] Maastricht Univ, Dept Psychiat & Neuropsychol, NL-6200 MD Maastricht, Netherlands. [Walters, Lawrence] Enova Int Inc, Software Engn, Chicago, IL 60604 USA. [Wilson, Gail W. T.] Oklahoma State Univ, Dept Nat Resource Ecol & Management, Stillwater, OK 74078 USA. [Zee, Peter C.] Calif State Univ Northridge, Dept Biol, Northridge, CA 91330 USA. RP Chaudhary, VB (reprint author), Depaul Univ, Dept Environm Sci & Studies, Chicago, IL 60614 USA. EM veerbala@hotmail.com RI Moore, John/E-9802-2011; Viechtbauer, Wolfgang/M-1549-2013; OI Viechtbauer, Wolfgang/0000-0003-3463-4063; Rua, Megan A./0000-0002-2883-2795 NR 46 TC 3 Z9 3 U1 8 U2 8 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-4463 J9 SCI DATA JI Sci. Data PD MAY 10 PY 2016 VL 3 AR UNSP 160028 DI 10.1038/sdata.2016.28 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EF3HJ UT WOS:000390215100003 PM 27163938 ER PT J AU Pawlak, EA Noah, TL Zhou, HB Chehrazi, C Robinette, C Diaz-Sanchez, D Muller, L Jaspers, I AF Pawlak, Erica A. Noah, Terry L. Zhou, Haibo Chehrazi, Claire Robinette, Carole Diaz-Sanchez, David Muller, Loretta Jaspers, Ilona TI Diesel exposure suppresses natural killer cell function and resolution of eosinophil inflammation: a randomized controlled trial of exposure in allergic rhinitics SO PARTICLE AND FIBRE TOXICOLOGY LA English DT Article DE Natural killer cell; Diesel exhaust; Eosinophil; Resolution of inflammation ID HOUSE-DUST MITE; EXHAUST PARTICLES; AIRWAY INFLAMMATION; ATTENUATED INFLUENZA; LUNG-DISEASE; IN-VITRO; NK CELLS; MICE; EXPRESSION; CHALLENGE AB Exposure to diesel exhaust (DE) is known to exacerbate allergic inflammation, including virus-induced eosinophil activation in laboratory animals. We have previously shown that in human volunteers with allergic rhinitis a short-term exposure to DE prior to infection with the live attenuated influenza virus (LAIV) increases markers of allergic inflammation in the nasal mucosa. Specifically, levels of eosinophilic cationic protein (ECP) were significantly enhanced in individuals exposed to DE prior to inoculation with LAIV and this effect was maintained for at least seven days. However, this previous study was limited in its scope of nasal immune endpoints and did not explore potential mechanisms mediating the prolonged exacerbation of allergic inflammation caused by exposure to DE prior to inoculation with LAIV. In this follow-up study, the methods were modified to expand experimental endpoints and explore the potential role of NK cells. The data presented here suggest DE prolongs viral-induced eosinophil activation, which was accompanied by decreased markers of NK cell recruitment and activation. Separate in vitro studies showed that exposure to DE particles decreases the ability of NK cells to kill eosinophils. Taken together, these follow-up studies suggest that DE-induced exacerbation of allergic inflammation in the context of viral infections may be mediated by decreased activity of NK cells and their ability to clear eosinophils. C1 [Pawlak, Erica A.; Noah, Terry L.; Robinette, Carole; Jaspers, Ilona] Univ N Carolina, Ctr Environm Med Asthma & Lung Biol, 104 Mason Farm Rd,Campus Box 7310, Chapel Hill, NC 27599 USA. [Noah, Terry L.; Jaspers, Ilona] Univ N Carolina, Dept Pediat, Chapel Hill, NC USA. [Zhou, Haibo] Univ N Carolina, Dept Biostat, Chapel Hill, NC USA. [Chehrazi, Claire] Univ N Carolina, Dept Med, Chapel Hill, NC USA. [Diaz-Sanchez, David] US EPA, Chapel Hill, NC USA. [Muller, Loretta] Univ Childrens Hosp, Basel, Switzerland. RP Jaspers, I (reprint author), Univ N Carolina, Ctr Environm Med Asthma & Lung Biol, 104 Mason Farm Rd,Campus Box 7310, Chapel Hill, NC 27599 USA.; Jaspers, I (reprint author), Univ N Carolina, Dept Pediat, Chapel Hill, NC USA. EM ilona_jaspers@med.unc.edu FU NIH [R01 ES013611]; United States Environmental Protection Agency [CR83346301]; Center for Environmental Medicine Asthma and Lung Biology at the University of North Carolina Chapel Hill [CR83346301]; US EPA FX This research was funded by an NIH R01 ES013611 to IJ and TN. This research was additionally supported by cooperative agreement CR83346301 between the United States Environmental Protection Agency and the Center for Environmental Medicine Asthma and Lung Biology at the University of North Carolina Chapel Hill. Though this work was funded in part by the US EPA, it does not necessarily reflect the view of the Agency and no official endorsement should be inferred. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 30 TC 0 Z9 0 U1 2 U2 4 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-8977 J9 PART FIBRE TOXICOL JI Part. Fibre Toxicol. PD MAY 6 PY 2016 VL 13 AR 24 DI 10.1186/s12989-016-0135-7 PG 8 WC Toxicology SC Toxicology GA DN6MH UT WOS:000377188500001 PM 27154411 ER PT J AU Le, CF Lehrter, JC Schaeffer, BA Hu, CM Murrell, MC Hagy, JD Greene, RM Beck, M AF Le, Chengfeng Lehrter, John C. Schaeffer, Blake A. Hu, Chuanmin Murrell, Michael C. Hagy, James D. Greene, Richard M. Beck, Marcus TI Bio-optical water quality dynamics observed from MERIS in Pensacola Bay, Florida SO ESTUARINE COASTAL AND SHELF SCIENCE LA English DT Article DE Bio-optical water quality; Estuary; Remote sensing; MERIS; Pensacola bay ID DISSOLVED ORGANIC-MATTER; REMOTE-SENSING REFLECTANCE; CHLOROPHYLL-A CONCENTRATION; APPARENT OPTICAL-PROPERTIES; CHESAPEAKE BAY; OCEAN COLOR; TAMPA BAY; IMAGING SPECTROMETER; SUSPENDED SEDIMENT; ESTUARINE WATERS AB Observed bio-optical water quality data collected from 2009 to 2011 in Pensacola Bay, Florida were used to develop empirical remote sensing retrieval algorithms for chlorophyll a (Chla), colored dissolved organic matter (CDOM), and suspended particulate matter (SPM). Time-series of the three bio-optical water quality variables were generated from MEdium Resolution Imaging Spectrometer (MERIS) observations from 2003 to 2011. Bio-optical water quality in this estuary exhibited spatial and temporal variations that were correlated to river discharge and wind. Both annual mean and monthly mean bio-optical water quality variables were positively correlated to river discharge. Monthly mean bio-optical water quality variables were also positively correlated to wind speed and wind density (defined by the number of days with daily mean wind speed > 3 m s(-1) in a month) over this estuary. These results indicate that bio-optical water quality dynamics in this estuary are vulnerable to changes in river discharge and river constituent loads and local weather conditions such as winter storms and hurricanes. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Le, Chengfeng] Zhejiang Univ, Ocean Coll, Hangzhou, Zhejiang, Peoples R China. [Le, Chengfeng; Lehrter, John C.; Murrell, Michael C.; Hagy, James D.; Greene, Richard M.; Beck, Marcus] US EPA, Gulf Ecol Div, Gulf Breeze, FL USA. [Schaeffer, Blake A.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Hu, Chuanmin] Univ S Florida, Coll Marine Sci, St Petersburg, FL 33701 USA. RP Le, CF (reprint author), Zhejiang Univ, Ocean Coll, Hangzhou, Zhejiang, Peoples R China. EM chengfengle@zju.edu.cn FU Oak Ridge institute for the Science and Education FX This work was supported in part by an appointment to the Research Participation Program for the U.S. Environmental Protection Agency, Office of Research and Development, administered by the Oak Ridge institute for the Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. NR 53 TC 0 Z9 0 U1 9 U2 21 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0272-7714 EI 1096-0015 J9 ESTUAR COAST SHELF S JI Estuar. Coast. Shelf Sci. PD MAY 5 PY 2016 VL 173 BP 26 EP 38 DI 10.1016/j.ecss.2016.02.003 PG 13 WC Marine & Freshwater Biology; Oceanography SC Marine & Freshwater Biology; Oceanography GA DL7HZ UT WOS:000375813100006 ER PT J AU Huynh, KA Siska, E Heithmar, E Tadjiki, S Pergantisi, SA AF Huynh, Khanh An Siska, Emily Heithmar, Edward Tadjiki, Soheyl Pergantisi, Spiros A. TI Detection and Quantification of Silver Nanoparticles at Environmentally Relevant Concentrations Using Asymmetric Flow Field-Flow Fractionation Online with Single Particle Inductively Coupled Plasma Mass Spectrometry SO ANALYTICAL CHEMISTRY LA English DT Article ID ICPMS; CAPABILITIES; TIME AB The presence of silver nanoparticles (AgNPs) in aquatic environments could potentially cause adverse impacts on ecosystems and human health. However, current understanding of the environmental fate and transport of AgNPs is still limited because their properties in complex environmental samples cannot be accurately determined. In this study, the feasibility of using asymmetric flow field-flow fractionation (AF4) connected online with single particle inductively coupled plasma mass spectrometry (spICPMS) to detect and quantify AgNPs at environmentally relevant concentrations was investigated. The AF4 channel had a thickness of 350 mu m and its accumulation wall was a 10 kDa regenerated cellulose membrane. A 0.02% FL-70 surfactant solution was used as an AF4 carrier. With 1.2 mL/min AF4 cross-flow rate, 1.5 mL/min AF4 channel flow rate, and 5 ms spICPMS dwell time, the AF4-spICPMS can detect and quantify 40-80 nm AgNPs, as well as Ag-SiO2 core shell nanoparticles (51.0 nm diameter Ag core and 21.6 nm SiO2 shell), with good recovery within 30 min. This system was not only effective in differentiating and quantifying different types of AgNPs with similar hydrodynamic diameters, such as in mixtures containing Ag-SiO2 core shell nanoparticles and 40-80 nm AgNPs, but also suitable for differentiating between 40 nm AgNPs and elevated Ag+ content. The study results indicate that AF4-spICPMS is capable of detecting and quantifying AgNPs and other engineered metal nanomaterials in environmental samples. Nevertheless, further studies are needed before AF4-spICPMS can become a routine analytical technique. C1 [Huynh, Khanh An] US EPA, Natl Res Council Postdoctoral Associate, Natl Exposure Res Lab, Exposure Methods & Measurement Div,Environm Chem, 944 East Harmon Ave, Las Vegas, NV 89119 USA. [Siska, Emily] Univ Nevada, Dept Chem, 4505 Maryland Pkwy, Las Vegas, NV 89154 USA. [Heithmar, Edward] US EPA, Natl Exposure Res Lab, Exposure Methods & Measurement Div, Environm Chem Branch, 944 East Harmon Ave, Las Vegas, NV 89119 USA. [Tadjiki, Soheyl] Postnova Analyt Inc, Salt Lake City, UT 84102 USA. [Pergantisi, Spiros A.] Univ Crete, Dept Chem, Environm Chem Proc Lab, Voutes Campus, Iraklion 71003, Greece. RP Huynh, KA (reprint author), US EPA, Natl Res Council Postdoctoral Associate, Natl Exposure Res Lab, Exposure Methods & Measurement Div,Environm Chem, 944 East Harmon Ave, Las Vegas, NV 89119 USA. EM huynh.khanhan@epa.gov RI Huynh, Khanh An /P-3928-2016 FU National Research Council Research Associateship Award at the U.S. Environmental Protection Agency in Las Vegas, NV FX Size distribution data of AgNPs and Ag-SiO2 NPs were provided by nanoComposix. We would like to thank anonymous reviewers for valuable comments and Dr. Frank von der Kammer for insightful discussions. This research was performed while KAH was a Vietnam Education Foundation fellow and held a National Research Council Research Associateship Award at the U.S. Environmental Protection Agency in Las Vegas, NV. NR 25 TC 5 Z9 5 U1 7 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 EI 1520-6882 J9 ANAL CHEM JI Anal. Chem. PD MAY 3 PY 2016 VL 88 IS 9 BP 4909 EP 4916 DI 10.1021/acs.analchem.6b00764 PG 8 WC Chemistry, Analytical SC Chemistry GA DR0ZV UT WOS:000379636600045 PM 27104795 ER PT J AU Teeguarden, JG Tan, YM Edwards, SW Leonard, JA Anderson, KA Corley, RA Kile, ML Simonich, SM Stone, D Tanguay, RL Waters, KM Harper, SL Williams, DE AF Teeguarden, Justin G. Tan, Yu-Mei Edwards, Stephen W. Leonard, Jeremy A. Anderson, Kim A. Corley, Richard A. Kile, Molly L. Simonich, Staci M. Stone, David Tanguay, Robert L. Waters, Katrina M. Harper, Stacey L. Williams, David E. TI Completing the Link between Exposure Science and Toxicology for Improved Environmental Health Decision Making: The Aggregate Exposure Pathway Framework SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID ADVERSE OUTCOME PATHWAYS; PERSONAL CARE PRODUCTS; CONSUMER EXPOSURE; RISK ASSESSMENTS; MODEL; DOSIMETRY; CHEMICALS; PESTICIDES; EMISSIONS; IMPACT C1 [Teeguarden, Justin G.; Corley, Richard A.; Waters, Katrina M.] Pacific NW Natl Lab, Hlth Effects & Exposure Sci, Richland, WA 99352 USA. [Teeguarden, Justin G.; Anderson, Kim A.; Corley, Richard A.; Simonich, Staci M.; Stone, David; Tanguay, Robert L.; Waters, Katrina M.; Harper, Stacey L.; Williams, David E.] Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 93771 USA. [Kile, Molly L.] Oregon State Univ, Sch Biol & Populat Hlth Sci, Corvallis, OR 93771 USA. [Tan, Yu-Mei] US EPA, Natl Exposure Res Lab, Durham, NC 27709 USA. [Edwards, Stephen W.] US EPA, Natl Hlth & Environm Effects Res Lab, Durham, NC 27709 USA. [Leonard, Jeremy A.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Harper, Stacey L.] Oregon State Univ, Sch Chem Biol & Environm Engn, Corvallis, OR 97331 USA. RP Teeguarden, JG (reprint author), Pacific NW Natl Lab, Hlth Effects & Exposure Sci, Richland, WA 99352 USA.; Teeguarden, JG (reprint author), Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 93771 USA. EM justin.teeg-uarden@pnl.gov FU National Institute of Environmental Health Sciences [P42ES016465]; Laboratory Directed Research and Development program at the Pacific Northwest National Laboratory (PNNL); DOE [DE-AC05-76RLO 1830] FX This work (J.G.T.) was supported by P42ES016465 (Oregon State University-PNNL) by the National Institute of Environmental Health Sciences and the Laboratory Directed Research and Development program at the Pacific Northwest National Laboratory (PNNL) and is a contribution of the Global Forensic Chemical Exposure Assessment for the Environmental Exposome project. PNNL is a multiprogram national laboratory operated by Battelle for the DOE under Contract DE-AC05-76RLO 1830. The U.S. Environmental Protection Agency has provided administrative review and has approved for publication. NR 38 TC 8 Z9 8 U1 11 U2 47 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD MAY 3 PY 2016 VL 50 IS 9 BP 4579 EP 4586 DI 10.1021/acs.est.5b05311 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DL3GE UT WOS:000375521400001 PM 26759916 ER PT J AU Yost, EE Stanek, J DeWoskin, RS Burgoon, LD AF Yost, Erin E. Stanek, John DeWoskin, Robert S. Burgoon, Lyle D. TI Overview of Chronic Oral Toxicity Values for Chemicals Present in Hydraulic Fracturing Fluids, Flowback, and Produced Waters SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID NATURAL-GAS OPERATIONS; SHALE; PENNSYLVANIA; IMPACTS AB Concerns have been raised about potential public health effects that may arise if hydraulic fracturing related chemicals were to impact drinking water resources. This study presents an overview of the chronic oral toxicity values-specifically, chronic oral reference values (RfVs) for noncancer effects, and oral slope factors (OSFs) for cancer-that are available for a list of 1173 chemicals that the United States (U.S.) Environmental Protection Agency (EPA) identified as being associated with hydraulic fracturing, including 1076 chemicals used in hydraulic fracturing fluids and 134 chemicals detected in flowback or produced waters from hydraulically fractured wells. The EPA compiled RfVs and OSFs using six governmental and intergovernmental data sources. Ninety (8%) of the 1076 chemicals reported in hydraulic fracturing fluids and 83 (62%) of the 134 chemicals reported in flowback/produced water had a chronic oral RfV or OSF available from one or more of the six sources. Furthermore, of the 36 chemicals reported in hydraulic fracturing fluids in at least 10% of wells nationwide (identified from EPA's analysis of the FracFocus Chemical Disclosure Registry 1.0), 8 chemicals (22%) had an available chronic oral RfV. The lack of chronic oral RfVs and OSFs for the majority of these chemicals highlights the significant knowledge gap that exists to assess the potential human health hazards associated with hydraulic fracturing. C1 [Yost, Erin E.] US EPA, Oak Ridge Inst Sci & Educ ORISE Res Participat, Natl Ctr Environm Assessment, Res Triangle Pk, Res Triangle Pk, NC 27711 USA. [Yost, Erin E.; Stanek, John; DeWoskin, Robert S.; Burgoon, Lyle D.] US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Res Triangle Pk, Res Triangle Pk, NC 27711 USA. [DeWoskin, Robert S.] etioLogic LLC, Durham, NC 27712 USA. [Burgoon, Lyle D.] US Army Engineer Res & Dev Ctr, Environm Lab, Res Triangle Pk, Res Triangle Pk, NC 27711 USA. RP Burgoon, LD (reprint author), US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Res Triangle Pk, Res Triangle Pk, NC 27711 USA. EM Lyle.D.Burgoon@usace.army.mil OI Burgoon, Lyle/0000-0003-4977-5352 NR 37 TC 6 Z9 6 U1 16 U2 40 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD MAY 3 PY 2016 VL 50 IS 9 BP 4788 EP 4797 DI 10.1021/acs.est.5b04645 PG 10 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DL3GE UT WOS:000375521400023 PM 27050380 ER PT J AU Hughes, MF Ross, DG Edwards, BC DeVito, MJ Starr, JM AF Hughes, Michael F. Ross, David G. Edwards, Brenda C. DeVito, Michael J. Starr, James M. TI Tissue time course and bioavailability of the pyrethroid insecticide bifenthrin in the Long-Evans rat SO XENOBIOTICA LA English DT Article DE disposition; pharmacokinetics; pyrethroids; Bifenthrin ID P-GLYCOPROTEIN; PHARMACOKINETIC MODEL; ACUTE NEUROTOXICITY; TRANS-PERMETHRIN; MOTOR FUNCTION; BRAIN LEVELS; HUMAN MDR1; IN-VITRO; DELTAMETHRIN; METABOLISM AB 1. Pyrethroids are neurotoxic and parent pyrethroid appears to be toxic entity. This study evaluated the oral disposition and bioavailability of bifenthrin in the adult male Long-Evans rat. 2. In the disposition study, rats were administered bifenthrin (0.3 or 3 mg/kg) by oral gavage and serially sacrificed (0.25 h to 21 days). Blood, liver, brain and adipose tissue were removed. In the bioavailability study, blood was collected serially from jugular vein cannulated rats (0.25 to 24 h) following oral (0.3 or 3 mg/kg) or intravenous (0.3 mg/kg) administration of bifenthrin. Tissues were extracted and analyzed for bifenthrin by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). 3. Bifenthrin concentration in blood and liver peaked 1-2-h postoral administration and were approximately 90 ng/ml (or g) and 1000 ng/ml (or g) for both tissues at 0.3 and 3 mg/kg, respectively. Bifenthrin was rapidly cleared from both blood and liver. Brain concentrations peaked at 4-6 h and were lower than in blood at both doses (12 and 143 ng/g). Bifenthrin in adipose tissue peaked at the collected time points of 8 (157 ng/g) and 24 (1145 ng/g) h for the 0.3 and 3 mg/kg doses, respectively and was retained 21 days postoral administration. Following intravenous administration, the blood bifenthrin concentration decreased bi-exponentially, with a distribution half-life of 0.2 h and an elimination half-life of 8 h. Bifenthrin bioavailability was approximately 30%. These disposition and kinetic bifenthrin data may decrease uncertainties in the risk assessment for this pyrethroid insecticide. C1 [Hughes, Michael F.; Ross, David G.; Edwards, Brenda C.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [DeVito, Michael J.] NIEHS, Natl Toxicol Program, POB 12233, Res Triangle Pk, NC 27709 USA. [Starr, James M.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. RP Hughes, MF (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. EM hughes.michaelf@epa.gov FU U.S. Environmental Protection Agency FX The authors gratefully acknowledge Drs. E. Kenyon, J.E. Simmons and M. Tornero-Velez for their comments on a previous version of this manuscript. The authors thank Ms. J. Hutchison for her technical assistance in this study. All research was funded internally by the U.S. Environmental Protection Agency. NR 47 TC 0 Z9 0 U1 3 U2 16 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0049-8254 EI 1366-5928 J9 XENOBIOTICA JI Xenobiotica PD MAY 3 PY 2016 VL 46 IS 5 BP 430 EP 438 DI 10.3109/00498254.2015.1081710 PG 9 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA DE4SU UT WOS:000370621300006 PM 26367082 ER PT J AU Brown, RA Borst, M AF Brown, Robert A. Borst, Michael TI Evaluating the Accuracy of Common Runoff Estimation Methods for New Impervious Hot-Mix Asphalt SO JOURNAL OF SUSTAINABLE WATER IN THE BUILT ENVIRONMENT LA English DT Article DE Runoff; Hot-mix asphalt; Stormwater; Small storm hydrology method; Simple method; SCS-curve number method ID CURVE NUMBERS; CONCRETE AB Accurately predicting runoff volume from impervious surfaces for water quality design events is an important step to meet water quality and infiltration design targets for green infrastructure stormwater control measures. The objectives of this research were to quantify abstraction from a recently paved impervious hot-mix asphalt (HMA) parking lot surface and evaluate the accuracy in modeling runoff volume from small events using the following runoff estimation methods: the Soil Conservation Service Curve Number (SCS-CN) method, the Simple Method (SM), and the Small Storm Hydrology Method (SSHM). A 4,000 m(2) (0.4-ha) parking lot in Edison, New Jersey, was designed with impervious HMA driving lanes evenly draining onto parking lanes constructed with permeable pavement. Ten lined permeable pavement sections that capture all infiltrating water and route it to collection tanks were included in this study. Using a water balance approach on an event basis, the measured infiltrate volume was compared to the rainfall volume across the drainage area to determine the rainfall retained by the HMA surface and in the permeable pavement strata and underlying aggregate. Only events with an antecedent dry period (ADP) less than 24 h (N = 16) were used in this analysis because it minimized evaporation and additional storage in the permeable pavement profile. It was assumed that the rainfall retention depth for these events was completely abstracted in the HMA surface. In comparing measured retention in the HMA surface to the three runoff estimation methods, the SCS-CN method overpredicted abstraction in half of the test sections, and the SM and SSHM underpredicted abstraction in 8 of the 10 test sections. While evaporation from the permeable pavement profile from the events analyzed was small, it was not zero, so the measured rainfall retention depth had a small positive bias. After correcting for this bias, the results shifted closer to the predictions using the SM and SSHM and farther from the predictions using the SCS-CN method. The average and interquartile range (IQR) of the corrected depression storage depth in the HMA surface for the 10 test sections were 2.0 mm and 0.9-3.0 mm, respectively. For a 25.4-mm water quality design event, the predicted abstraction depth by the SM was within the IQR, the SSHM prediction was 0.1 mm below the IQR, and the SCS-CN prediction exceeded the IQR by 2.3 mm. The runoff predicted by the SCS-CN method for this example water quality event was about 15% less than the average and about 10% less than the IQR bound. (C) 2015 American Society of Civil Engineers. C1 [Brown, Robert A.] US EPA, Oak Ridge Inst Sci & Educ, 2890 Woodbridge Ave,MS 104, Edison, NJ 08837 USA. [Borst, Michael] US EPA, 2890 Woodbridge Ave,MS 104, Edison, NJ 08837 USA. RP Brown, RA (reprint author), US EPA, Oak Ridge Inst Sci & Educ, 2890 Woodbridge Ave,MS 104, Edison, NJ 08837 USA. EM rob.brown.rab@gmail.com; borst.mike@epa.gov FU U.S. Department of Energy; U.S. Environmental Protection Agency FX This project was supported in part by an appointment to the Research Participation Program at the National Risk Management Research Laboratory administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy and U.S. Environmental Protection Agency. The parking lot was constructed as a cooperative project with EPA's Office of Administration and Resources Management and Region 2. The authors would like to thank Ms. Lisa Cherry for organizing and analyzing the water level data; PARS Environmental, Inc. for conducting depth-to-volume measurements and providing on-site support; and Johnson, Mirmiran, and Thompson, Inc. for conducting the high-definition survey and assisting with interpretation of the output. NR 23 TC 0 Z9 0 U1 0 U2 1 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 2379-6111 J9 J SUSTAIN WATER BUIL JI J. Sustain. Water Built Environ. PD MAY PY 2016 VL 2 IS 2 AR 04015010 DI 10.1061/JSWBAY.0000806 PG 7 WC Water Resources SC Water Resources GA EJ1BT UT WOS:000392945900002 ER PT J AU Gwinn, MR AF Gwinn, M. R. TI Overview of Nanomaterial Regulation: Data Gaps and Research Needs for Risk Assessment SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Gwinn, M. R.] US EPA, Washington, DC 20460 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA S44 BP 373 EP 373 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100050 ER PT J AU Browne, P AF Browne, P. TI A Performance-Based Approach for Validating Computational Tools for Developmental and Reproductive Toxicity SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Browne, P.] US EPA, Washington, DC 20460 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA W5 BP 379 EP 379 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100062 ER PT J AU Simmons, S AF Simmons, S. TI A High-Throughput Screening Assay to Detect Thyroperoxidase Inhibitors and Discover Structural Alerts SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Simmons, S.] US EPA, ORD, NCCT, Res Triangle Pk, NC 27711 USA. NR 0 TC 0 Z9 0 U1 5 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA W6 BP 380 EP 380 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100063 ER PT J AU Taylor, LG AF Taylor, L. G. TI What Is US Food and Drug Administration Looking for to Assess and Label Risk? SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Taylor, L. G.] US EPA, Silver Spring, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA W10 BP 382 EP 382 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100067 ER PT J AU Mckenzie, M Amosu, M Wu, X Wallace, S Henderson, M Bian, X Lu, K Stice, S Smith, MA AF Mckenzie, M. Amosu, M. Wu, X. Wallace, S. Henderson, M. Bian, X. Lu, K. Stice, S. Smith, M. A. TI Determining Developmental Neurotoxicity of Pesticides Utilizing Metabolomic Profile from Neural Progenitor Cells SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Mckenzie, M.; Amosu, M.; Wu, X.; Lu, K.; Smith, M. A.] Univ Georgia, Environm Hlth Sci, Athens, GA 30602 USA. [Mckenzie, M.; Amosu, M.; Wu, X.; Bian, X.; Lu, K.; Stice, S.; Smith, M. A.] Univ Georgia, Regenerat Biosci Ctr, Athens, GA 30602 USA. [Wallace, S.] ArunA Biomed Inc, Athens, GA USA. [Henderson, M.] US EPA, Athens, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA 6 BP 387 EP 387 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100075 ER PT J AU Leung, MCK Kapraun, DF Williams, AJ Knudsen, TB AF Leung, M. C. K. Kapraun, D. F. Williams, A. J. Knudsen, T. B. TI An Evaluation of ToxCast Angiogenic Disruptors for Effects on Mitochondrial Bioactivity Profiles SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Leung, M. C. K.; Kapraun, D. F.; Williams, A. J.; Knudsen, T. B.] US EPA, Res Triangle Pk, NC 27711 USA. [Leung, M. C. K.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA 10 BP 389 EP 389 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100079 ER PT J AU Euling, S Makris, S Walker, T Gray, LE Iyer, P AF Euling, S. Makris, S. Walker, T. Gray, L. E., Jr. Iyer, P. TI A Systematic Review of Transgenerational Toxicological Studies after Gestational Exposure to Environmental Chemicals SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Euling, S.; Makris, S.; Walker, T.] US EPA, NCEA, ORD, Washington, DC 20460 USA. [Gray, L. E., Jr.] US EPA, NHEERL, TAD, Res Triangle Pk, NC 27711 USA. [Iyer, P.] OEHHA, Calif EPA, Sacramento, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA P18 BP 412 EP 412 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100122 ER PT J AU Euling, S Glenn, B Alcala, C AF Euling, S. Glenn, B. Alcala, C. TI The Role of Environmental Factors in Pubertal Gynecomastia SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Euling, S.; Glenn, B.; Alcala, C.] US EPA, NCEA, ORD, Washington, DC 20460 USA. NR 0 TC 0 Z9 0 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA P28 BP 417 EP 417 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100132 ER PT J AU Narotsky, MG Manley, AL Ola, O AF Narotsky, M. G. Manley, A. L. Ola, O. TI Assessment of Iodoform Effects on Pregnancy Maintenance and Eye Malformations in a Developmental Toxicity Screen with F344 Rats SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Narotsky, M. G.] US EPA, Res Triangle Pk, NC 27711 USA. [Manley, A. L.] ORISE, Oak Ridge, TN USA. [Ola, O.] Student Serv Contractor, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA P31 BP 418 EP 418 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100135 ER PT J AU Wolf, CJ Becker, C Das, KP Watkins, AM Belair, DG Abbott, BD AF Wolf, C. J. Becker, C. Das, K. P. Watkins, A. M. Belair, D. G. Abbott, B. D. TI Development of a 3-D Co-Culture Model Using Human Stem Cells for Studying Embryonic Palatal Fusion SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Wolf, C. J.; Becker, C.; Das, K. P.; Watkins, A. M.; Belair, D. G.; Abbott, B. D.] US EPA, Res Triangle Pk, NC 27711 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2016 VL 106 IS 5 SI SI MA P32 BP 419 EP 419 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DQ2HT UT WOS:000379024100136 ER PT J AU Clarke, L McFarland, J Octaviano, C van Ruijven, B Beach, R Daenzer, K Martinez, SH Lucena, AFP Kitous, A Labriet, M Rodriguez, AML Mundra, A van der Zwaan, B AF Clarke, Leon McFarland, James Octaviano, Claudia van Ruijven, Bas Beach, Robert Daenzer, Kathryn Martinez, Sara Herreras Lucena, Andre F. P. Kitous, Alban Labriet, Maryse Loboguerrero Rodriguez, Ana Maria Mundra, Anupriya van der Zwaan, Bob TI Long-term abatement potential and current policy trajectories in Latin American countries SO ENERGY ECONOMICS LA English DT Article DE Scenarios; Latin America; Climate mitigation ID SCENARIOS; STABILIZATION; STRATEGIES; TARGETS; FUTURE; SYSTEM; MODEL AB This paper provides perspectives on the role of Latin American and Latin American countries in meeting global abatement goals, based on the scenarios developed through the CLIMACAP-LAMP modeling study. Abatement potential in Latin America, among other things, is influenced by its development status, the large contributions of non-CO2 and land use change CO2 emissions, and energy endowments. In most scenarios in this study, the economic potential to reduce fossil fuel CO2 as well as non-CO2 emissions in Latin America in 2050 is lower than in the rest of the world (in total) when measured against 2010 emissions, due largely to higher emission growth in Latin America than in the rest of the world in the absence of abatement. The potential to reduce land use change CO2 emissions is complicated by a wide range of factors and is not addressed in this paper (land use emissions are largely addressed in a companion paper). The study confirms the results of previous research that the variation in abatement costs across models may vary by an order of magnitude or more, limiting the value of these assessments and supporting continued calls for research on the degree to which models are effectively representing key local circumstances that influence costs and available abatement options. Finally, a review of policies in place in several Latin American countries at the time of this writing finds that they would be of varying success in meeting the emission levels proposed by the most recent IPCC reports to limit global temperature change to 2 degrees C. Published by Elsevier B.V. C1 [Clarke, Leon; Mundra, Anupriya] Pacific NW Natl Lab, Joint Global Change Res Inst, Richland, WA 99352 USA. [McFarland, James] US EPA, Washington, DC 20460 USA. [Octaviano, Claudia] MIT, Joint Program Sci & Policy Global Change, Cambridge, MA 02139 USA. [van Ruijven, Bas] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. [Beach, Robert] Res Triangle Inst, POB 12194, Res Triangle Pk, NC 27709 USA. [Daenzer, Kathryn] Penn State Univ, University Pk, PA 16802 USA. [Martinez, Sara Herreras] Netherlands Environm Assessment Agcy, Bilthoven, Netherlands. [Lucena, Andre F. P.] Univ Fed Rio de Janeiro, Energy Planning Program, BR-21941 Rio De Janeiro, Brazil. [Kitous, Alban] Commiss European Communities, Joint Res Ctr, I-21020 Ispra, Italy. [Labriet, Maryse] Eneris Environm Energy Consultants, Madrid, Spain. [Loboguerrero Rodriguez, Ana Maria] CGIAR Res Program Climate Change Agr & Food Secur, Cali, Colombia. [van der Zwaan, Bob] Energy Res Ctr Netherlands ECN, Policy Studies, Amsterdam, Netherlands. [van der Zwaan, Bob] Johns Hopkins Univ, Sch Adv Int Studies, Bologna, Italy. [van der Zwaan, Bob] Univ Amsterdam, Fac Sci HIMS, Amsterdam, Netherlands. RP Clarke, L (reprint author), Pacific NW Natl Lab, Joint Global Change Res Inst, Richland, WA 99352 USA. EM leon.clarke@pnnl.gov OI Loboguerrero, Ana Maria/0000-0003-2690-0763 FU European Union [EuropeAid/131944/C/SER/Multi]; U.S. Agency for International Development; U.S. Environmental Protection Agency [DW89923040, DW89923951US]; National Science Foundation [1243095] FX The research that allowed the publication of this paper has been produced with the financial assistance of the European Union in the context of the CLIMACAP project (EuropeAid/131944/C/SER/Multi) and of the U.S. Agency for International Development and U.S. Environmental Protection Agency in the context of the LAMP project (under Interagency Agreements DW89923040 and DW89923951US). Additional research support for individual authors includes van Ruijven (National Science Foundation, Grant No. 1243095) and Daenzer (U.S. EPA). The contents of this publication are the sole responsibility of the authors and can in no way be taken to reflect the views of the European Union or the U.S. government. The authors would like to thank the feedback and efforts from all CLIMACAP and LAMP project partners for enabling the research results reported in this article. NR 35 TC 6 Z9 6 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0140-9883 EI 1873-6181 J9 ENERG ECON JI Energy Econ. PD MAY PY 2016 VL 56 BP 513 EP 525 DI 10.1016/j.eneco.2016.01.011 PG 13 WC Economics SC Business & Economics GA DP4DO UT WOS:000378446600046 ER PT J AU Veysey, J Octaviano, C Calvin, K Martinez, SH Kitous, A McFarland, J van der Zwaan, B AF Veysey, Jason Octaviano, Claudia Calvin, Katherine Martinez, Sara Herreras Kitous, Alban McFarland, James van der Zwaan, Bob TI Pathways to Mexico's climate change mitigation targets: A multi-model analysis SO ENERGY ECONOMICS LA English DT Article DE Climate policy; Mexico; Modeling; Mitigation; Mitigation pathway AB Mexico's climate policy sets ambitious national greenhouse gas (GHG) emission reduction targets-30% versus a business-as-usual baseline by 2020, 50% versus 2000 by 2050. However, these goals are at odds with recent energy and emission trends in the country. Both energy use and GHG emissions in Mexico have grown substantially over the last two decades. We investigate how Mexico might reverse current trends and reach its mitigation targets by exploring results from energy system and economic models involved in the CLIMACAP-LAMP project. To meet Mexico's emission reduction targets, all modeling groups agree that decarbonization of electricity is needed, along with changes in the transport sector, either to more efficient vehicles or a combination of more efficient vehicles and lower carbon fuels. These measures reduce GHG emissions as well as emissions of other air pollutants. The models find different energy supply pathways, with some solutions based on renewable energy and others relying on biomass or fossil fuels with carbon capture and storage. The economy-wide costs of deep mitigation could range from 2% to 4% of GDP in 2030, and from 7% to 15% of GDP in 2050. Our results suggest that Mexico has some flexibility in designing deep mitigation strategies, and that technological options could allow Mexico to achieve its emission reduction targets, albeit at a cost to the country. (C) 2016 Battelle Memorial Institute and The Authors. Published by Elsevier B.V. C1 [Veysey, Jason] Stockholm Environm Inst, 11 Curtis Ave, Somerville, MA 02144 USA. [Octaviano, Claudia] MA Inst Technol, Joint Program Sci & Policy Global Change, Cambridge, MA USA. [Octaviano, Claudia] Mario Molina Ctr Strateg Studies Energy & Environ, Mexico City, DF, Mexico. [Calvin, Katherine] Univ Maryland, Pacific NW Natl Lab, Joint Global Change Res Inst, 5825 Univ Res Ct,Suite 3500, College Pk, MD 20740 USA. [Martinez, Sara Herreras] Univ Utrecht, Copernicus Inst Sustainable Dev, Heidelberglaan 2, NL-3584 CS Utrecht, Netherlands. [Kitous, Alban] European Commiss, DG Joint Res Ctr JRC, IPTS, Unit Econ Climate Change Energy & Transport J1, Edificio Expo,C Inca Garcilaso 3, E-41092 Seville, Spain. [McFarland, James] US EPA, USEPA Headquarters, William Jefferson Clinton Bldg,1200 Penn Ave NW, Washington, DC 20460 USA. [van der Zwaan, Bob] Energy Res Ctr Netherlands ECN, Policy Studies, Radarweg 60, NL-1043 NT Amsterdam, Netherlands. [van der Zwaan, Bob] Johns Hopkins Univ, Sch Adv Int Studies, Bologna, Italy. [van der Zwaan, Bob] Univ Amsterdam, Fac Sci, Amsterdam, Netherlands. RP Veysey, J (reprint author), Stockholm Environm Inst, 11 Curtis Ave, Somerville, MA 02144 USA. EM jason.veysey@sei-us.org; claus@mit.edu; katherine.calvin@pnnl.gov; S.D.HerrerasMartinez@uu.nl; alban.kitous@ec.europa.eu; mcfarland.james@epa.gov; vanderzwaan@ecn.nl FU European Union [EuropeAid/131944/C/SER/Multi]; U.S. Agency for International Development [DW89923040, DW89923951US]; U.S. Environmental Protection Agency [DW89923040, DW89923951US] FX The research that allowed the publication of this paper has been produced with the financial assistance of the European Union in the context of the CLIMACAP project (EuropeAid/131944/C/SER/Multi) and of the U.S. Agency for International Development and U.S. Environmental Protection Agency in the context of the LAMP project (under Interagency Agreements DW89923040 and DW89923951US). The contents of this publication are the sole responsibility of the authors and can in no way be taken to reflect the views of the European Union or the U.S. government The authors would like to thank all CLIMACAP and LAMP project partners for their feedback and efforts to enable the research results reported in this article. NR 31 TC 5 Z9 5 U1 5 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0140-9883 EI 1873-6181 J9 ENERG ECON JI Energy Econ. PD MAY PY 2016 VL 56 BP 587 EP 599 DI 10.1016/j.eneco.2015.04.011 PG 13 WC Economics SC Business & Economics GA DP4DO UT WOS:000378446600052 ER PT J AU Kober, T Summerton, P Pollitt, H Chewpreecha, U Ren, XL Wills, W Octaviano, C McFarland, J Beach, R Cai, YX Calderon, S Fisher-Vanden, K Rodriguez, AML AF Kober, Tom Summerton, Philip Pollitt, Hector Chewpreecha, Unnada Ren, Xiaolin Wills, William Octaviano, Claudia McFarland, James Beach, Robert Cai, Yongxia Calderon, Silvia Fisher-Vanden, Karen Loboguerrero Rodriguez, Ana Maria TI Macroeconomic impacts of climate change mitigation in Latin America: A cross-model comparison SO ENERGY ECONOMICS LA English DT Article DE Climate policy; Energy and economy models; GDP; Employment AB In this paper we analyse macroeconomic consequences of greenhouse gas emission mitigation in Latin America up to 2050 through a multi-model comparison approach undertaken in the context of the CLIMACAP LAMP research project. We compare two carbon tax scenarios with a business-as-usual scenario of anticipated future energy demand. In the short term, with carbon prices reaching around $15/tCO(2) by 2030, most models agree that the reduction in consumer spending, as a proxy for welfare, is limited to about 03%. By 2050, at carbon prices of $165/tCO(2), there is much more divergence in the estimated impact on consumer spending as well as GDP across models and regions, which reflects uncertainties about technology costs and substitution opportunities between technologies. We observe that the consequences of increasingly higher carbon prices, in terms of reduced consumer spending and GDP, tend to be fairly linear with the carbon price in our CGE models. However, the consequences are divergent and nonlinear in our econometric model, that is linked to an energy system model that simulates step-changes in technology substitution. The results of one model show that climate policy measures can have positive effects on consumer spending and GDP, which results from an investment stimulus and the redistribution of carbon price revenues to consumers. (C) 2016 The Authors. Published by Elsevier B.V. C1 [Kober, Tom] Energy Res Ctr Netherlands, Policy Studies, Amsterdam, Netherlands. [Summerton, Philip; Pollitt, Hector; Chewpreecha, Unnada] Cambridge Econometr, Cambridge, England. [Ren, Xiaolin] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. [Wills, William] Univ Fed Rio de Janeiro, Grad Sch Engn, Energy Planning Program, BR-21941 Rio De Janeiro, Brazil. [Octaviano, Claudia] MIT, 77 Massachusetts Ave, Cambridge, MA 02139 USA. [McFarland, James] US EPA, Washington, DC 20460 USA. [Beach, Robert; Cai, Yongxia] RTI Int, Res Triangle Pk, NC USA. [Calderon, Silvia] Dept Nacl Planeac, Bogota, Colombia. [Fisher-Vanden, Karen] Penn State Univ, University Pk, PA 16802 USA. [Loboguerrero Rodriguez, Ana Maria] CGIAR Res Program Climate Change Agr & Food Secur, Cali, Colombia. [Loboguerrero Rodriguez, Ana Maria] Ctr Int Agr Trop, Apartado Aereo 6713, Cali, Colombia. RP Kober, T (reprint author), Energy Res Ctr Netherlands, Policy Studies, Amsterdam, Netherlands. EM kober@ecn.nl OI Loboguerrero, Ana Maria/0000-0003-2690-0763 FU European Union [EuropeAid/131944/C/SER/Multi]; U.S. Agency for International Development [DW89923040, DW89923951US]; U.S. Environmental Protection Agency [DW89923040, DW89923951US] FX The research that allowed the publication of this paper has been produced with the financial assistance of the European Union in the context of the CLIMACAP project (EuropeAid/131944/C/SER/Multi) and the financial aid of the U.S. Agency for International Development and U.S. Environmental Protection Agency in the context of the LAMP project (under interagency agreements DW89923040 and DW89923951US). The contents of this publication are the sole responsibility of the authors and can in no way be taken to reflect the views of the European Union or the U.S. government. The authors would like to thank the feedback and efforts from all CLIMACAP-LAMP project partners for enabling the research results reported in this article. NR 12 TC 1 Z9 1 U1 9 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0140-9883 EI 1873-6181 J9 ENERG ECON JI Energy Econ. PD MAY PY 2016 VL 56 BP 625 EP 636 DI 10.1016/j.eneco.2016.02.002 PG 12 WC Economics SC Business & Economics GA DP4DO UT WOS:000378446600055 ER PT J AU Ko, DS Gould, RW Penta, B Lehrter, JC AF Ko, Dong S. Gould, Richard W., Jr. Penta, Bradley Lehrter, John C. TI Impact of Satellite Remote Sensing Data on Simulations of Coastal Circulation and Hypoxia on the Louisiana Continental Shelf SO REMOTE SENSING LA English DT Article DE TRMM; MODIS; Louisiana shelf; coastal hypoxia; NCOM-LCS ocean model ID GULF-OF-MEXICO; PREDICTION SYSTEM; RETROSPECTIVE ANALYSIS; DATA ASSIMILATION; OCEAN; MODEL; VARIABILITY; ATTENUATION; PLUME; ZONE AB We estimated surface salinity flux and solar penetration from satellite data, and performed model simulations to examine the impact of including the satellite estimates on temperature, salinity, and dissolved oxygen distributions on the Louisiana continental shelf (LCS) near the annual hypoxic zone. Rainfall data from the Tropical Rainfall Measurement Mission (TRMM) were used for the salinity flux, and the diffuse attenuation coefficient (Kd) from Moderate Resolution Imaging Spectroradiometer (MODIS) were used for solar penetration. Improvements in the model results in comparison with in situ observations occurred when the two types of satellite data were included. Without inclusion of the satellite-derived surface salinity flux, realistic monthly variability in the model salinity fields was observed, but important inter-annual variability was missed. Without inclusion of the satellite-derived light attenuation, model bottom water temperatures were too high nearshore due to excessive penetration of solar irradiance. In general, these salinity and temperature errors led to model stratification that was too weak, and the model failed to capture observed spatial and temporal variability in water-column vertical stratification. Inclusion of the satellite data improved temperature and salinity predictions and the vertical stratification was strengthened, which improved prediction of bottom-water dissolved oxygen. The model-predicted area of bottom-water hypoxia on the Louisiana shelf, an important management metric, was substantially improved in comparison to observed hypoxic area by including the satellite data. C1 [Ko, Dong S.; Gould, Richard W., Jr.; Penta, Bradley] Naval Res Lab, Div Oceanog, Stennis Space Ctr, MS 39529 USA. [Lehrter, John C.] US EPA, Off Res & Dev, Gulf Breeze, FL 32561 USA. RP Ko, DS (reprint author), Naval Res Lab, Div Oceanog, Stennis Space Ctr, MS 39529 USA. EM ko@nrlssc.navy.mil; gould@nrlssc.navy.mil; penta@nrlssc.navy.mil; lehrter.john@epa.gov OI Gould, Richard/0000-0002-5149-048X FU US EPA; Naval Research Laboratory FX This work was supported by an inter-agency agreement between the US EPA and the Naval Research Laboratory. Analyses and visualizations used in Figure 4 of this paper were produced with the Giovanni online data system, developed and maintained by the NASA GES DISC. We also acknowledge the TRMM mission scientists and associated NASA personnel for the production of the rainfall data used in this research effort. We thank for the useful comments and suggestions from 3 reviewers. The views expressed in this manuscript are those of the authors and do not necessarily reflect the views or policies of the US Environmental Protection Agency and the Naval Research Laboratory. NR 37 TC 1 Z9 1 U1 4 U2 6 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD MAY PY 2016 VL 8 IS 5 AR 435 DI 10.3390/rs8050435 PG 16 WC Remote Sensing SC Remote Sensing GA DP3PC UT WOS:000378406400079 ER PT J AU Kraft, AD Aschner, M Cory-Slechta, DA Bilbo, SD Caudle, WM Makris, SL AF Kraft, Andrew D. Aschner, Michael Cory-Slechta, Deborah A. Bilbo, Staci D. Caudle, W. Michael Makris, Susan L. TI Unmasking silent neurotoxicity following developmental exposure to environmental toxicants SO NEUROTOXICOLOGY AND TERATOLOGY LA English DT Article DE Neurotoxicity; Risk assessment; Delayed-onset; Development; Behavior; Environmental health ID PARKINSONS-DISEASE PHENOTYPE; PRENATAL AIR-POLLUTION; LEVEL LEAD-EXPOSURE; BRAIN-DEVELOPMENT; NEURODEGENERATIVE DISEASE; DOPAMINE SYSTEM; C-ELEGANS; METHYLMERCURY; STRESS; SCHIZOPHRENIA AB Silent neurotoxicity, a term introduced approximately 25 years ago, is defined as a persistent change to the nervous system that does not manifest as overt evidence of toxicity (i.e. it remains clinically unapparent) unless unmasked by experimental or natural processes. Silent neurotoxicants can be challenging for risk assessors, as the multifactorial experiments needed to reveal their effects are seldom conducted, and they are not addressed by current study design guidelines. This topic was the focus of a symposium addressing the interpretation and use of silent neurotoxicity data in human health risk assessments of environmental toxicants at the annual meeting of the Developmental Neurotoxicology Society (previously the Neurobehavioral Teratology Society) on June 30th, 2014. Several factors important to the design and interpretation of studies assessing the potential for silent neurotoxicity were discussed by the panelists and audience members. Silent neurotoxicity was demonstrated to be highly specific to the characteristics of the animals being examined, the unmasking agent tested, and the behavioral endpoint(s) evaluated. Overall, the experimental examples presented highlighted a need to consider common adverse outcomes and common biological targets for chemical and non-chemical stressors, particularly when the exposure and stressors are known to co-occur. Risk assessors could improve the evaluation of silent neurotoxicants in assessments through specific steps from researchers, including experiments to reveal the molecular targets and mechanisms that may result in specific types of silent neurotoxicity, and experiments with complex challenges reminiscent of the human situation. Published by Elsevier Inc. C1 [Kraft, Andrew D.; Makris, Susan L.] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Washington, DC 20460 USA. [Aschner, Michael] Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10467 USA. [Cory-Slechta, Deborah A.] Univ Rochester, Dept Environm Med, Sch Med, Rochester, NY USA. [Bilbo, Staci D.] Duke Univ, Dept Psychol, Durham, NC 27706 USA. [Bilbo, Staci D.] Duke Univ, Dept Neurosci, Durham, NC USA. [Caudle, W. Michael] Emory Univ, Dept Environm Hlth, Atlanta, GA 30322 USA. [Caudle, W. Michael] Emory Univ, Ctr Neurodegenerat Dis, Atlanta, GA 30322 USA. RP Kraft, AD (reprint author), 1200 Penn Ave NW,Mail Code 8623P, Washington, DC 20460 USA. EM kraft.andrew@epa.gov RI Bilbo, Staci/L-5076-2016 OI Bilbo, Staci/0000-0001-6736-7841 FU U.S. EPA [EP-14-H-000523] FX The authors would like to thank Drs. Laura Dishaw and Christina Powers (U.S. EPA) for their insightful reviews of this manuscript. This commentary is based on a symposium held at the Developmental Neurotoxicology Society (DNTS; previously the Neurobehavioral Teratology Society) annual meeting, held June 29-July 2, 2014 in Bellevue, WA. Funding was provided by the U.S. EPA, order EP-14-H-000523. The views expressed in this commentary are those of the authors and they do not represent the views or policies of the U.S. Environmental Protection Agency, the Albert Einstein College of Medicine, the University of Rochester Medical School, Duke University, Emory University, or the DNTS. NR 49 TC 0 Z9 0 U1 6 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0892-0362 EI 1872-9738 J9 NEUROTOXICOL TERATOL JI Neurotoxicol. Teratol. PD MAY-JUN PY 2016 VL 55 BP 38 EP 44 DI 10.1016/j.ntt.2016.03.005 PG 7 WC Neurosciences; Toxicology SC Neurosciences & Neurology; Toxicology GA DO6WC UT WOS:000377923200006 PM 27049787 ER PT J AU Mundy, W AF Mundy, William TI Phenotypic Screening for Developmental Neurotoxicity: Mechanistic Data at the Level of the Cell SO NEUROTOXICOLOGY AND TERATOLOGY LA English DT Meeting Abstract CT 40th Anniversary Annual Meeting of the Developmental-Neurotoxicology-Society Held in Conjunction with the 56th Annual Meeting of the Teratology-Society CY JUN 25-29, 2016 CL San Antonio, TX SP Dev Neurotoxicol Soc, Teratol Soc C1 [Mundy, William] US EPA, Integrated Syst Toxicol Div, Res Triangle Pk, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0892-0362 EI 1872-9738 J9 NEUROTOXICOL TERATOL JI Neurotoxicol. Teratol. PD MAY-JUN PY 2016 VL 55 MA DNTS 09 BP 64 EP 65 PG 2 WC Neurosciences; Toxicology SC Neurosciences & Neurology; Toxicology GA DO6WC UT WOS:000377923200016 ER PT J AU Yost, E Arzuaga, X Beverly, B Blessinger, T Euling, S Hotchkiss, A Makris, S Walker, T Weaver, A AF Yost, Erin Arzuaga, Xabier Beverly, Brandiese Blessinger, Todd Euling, Susan Hotchkiss, Andrew Makris, Susan Walker, Teneille Weaver, Andre TI Comparison of Developmental Effects Across Multiple Phthalate Esters SO NEUROTOXICOLOGY AND TERATOLOGY LA English DT Meeting Abstract CT 40th Anniversary Annual Meeting of the Developmental-Neurotoxicology-Society Held in Conjunction with the 56th Annual Meeting of the Teratology-Society CY JUN 25-29, 2016 CL San Antonio, TX SP Dev Neurotoxicol Soc, Teratol Soc C1 [Yost, Erin; Beverly, Brandiese; Hotchkiss, Andrew; Weaver, Andre] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Arzuaga, Xabier; Blessinger, Todd; Euling, Susan; Makris, Susan; Walker, Teneille] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Washington, DC 20460 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0892-0362 EI 1872-9738 J9 NEUROTOXICOL TERATOL JI Neurotoxicol. Teratol. PD MAY-JUN PY 2016 VL 55 MA P02 BP 71 EP 72 PG 2 WC Neurosciences; Toxicology SC Neurosciences & Neurology; Toxicology GA DO6WC UT WOS:000377923200041 ER PT J AU Macaulay, L Li, J Lebmann, G AF Macaulay, Laura Li, Jenny Lebmann, Geniece TI Prioritization of polychlorinated biphenyl congeners to support human health risk assessment SO NEUROTOXICOLOGY AND TERATOLOGY LA English DT Meeting Abstract CT 40th Anniversary Annual Meeting of the Developmental-Neurotoxicology-Society Held in Conjunction with the 56th Annual Meeting of the Teratology-Society CY JUN 25-29, 2016 CL San Antonio, TX SP Dev Neurotoxicol Soc, Teratol Soc C1 [Macaulay, Laura; Lebmann, Geniece] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Li, Jenny] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Washington, DC 20460 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0892-0362 EI 1872-9738 J9 NEUROTOXICOL TERATOL JI Neurotoxicol. Teratol. PD MAY-JUN PY 2016 VL 55 MA DNTS P05 BP 72 EP 73 PG 2 WC Neurosciences; Toxicology SC Neurosciences & Neurology; Toxicology GA DO6WC UT WOS:000377923200044 ER PT J AU Leonard, JA Tan, YM Gilbert, M Isaacs, K El-Masri, H AF Leonard, Jeremy A. Tan, Yu-Mei Gilbert, Mary Isaacs, Kristin El-Masri, Hisham TI Estimating Margin of Exposure to Thyroid Peroxidase Inhibitors Using High-Throughput in vitro Data, High-Throughput Exposure Modeling, and Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling SO TOXICOLOGICAL SCIENCES LA English DT Article DE thyroid peroxidase; adverse outcome pathway; margin of exposure; PBPK; PD model; high-throughput in vitro assay ID NEWLY DEVELOPED RADIOIMMUNOASSAY; ADVERSE OUTCOME PATHWAYS; THYROPEROXIDASE INHIBITION; TISSUE DISTRIBUTION; ANTITHYROID DRUGS; RAT; TRICLOSAN; HORMONES; PHARMACOKINETICS; METHIMAZOLE AB Some pharmaceuticals and environmental chemicals bind the thyroid peroxidase (TPO) enzyme and disrupt thyroid hormone production. The potential for TPO inhibition is a function of both the binding affinity and concentration of the chemical within the thyroid gland. The former can be determined through in vitro assays, and the latter is influenced by pharmacokinetic properties, along with environmental exposure levels. In this study, a physiologically based pharmacokinetic (PBPK) model was integrated with a pharmacodynamic (PD) model to establish internal doses capable of inhibiting TPO in relation to external exposure levels predicted through exposure modeling. The PBPK/PD model was evaluated using published serum or thyroid gland chemical concentrations or circulating thyroxine (T-4) and triiodothyronine (T-3) hormone levels measured in rats and humans. After evaluation, the model was used to estimate human equivalent intake doses resulting in reduction of T-4 and T-3 levels by 10% (ED10) for 6 chemicals of varying TPO-inhibiting potencies. These chemicals were methimazole, 6-propylthiouracil, resorcinol, benzophenone-2, 2-mercaptobenzothiazole, and triclosan. Margin of exposure values were estimated for these chemicals using the ED10 and predicted population exposure levels for females of child-bearing age. The modeling approach presented here revealed that examining hazard or exposure alone when prioritizing chemicals for risk assessment may be insufficient, and that consideration of pharmacokinetic properties is warranted. This approach also provides a mechanism for integrating in vitro data, pharmacokinetic properties, and exposure levels predicted through high-throughput means when interpreting adverse outcome pathways based on biological responses. C1 [Leonard, Jeremy A.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Leonard, Jeremy A.; Tan, Yu-Mei; Isaacs, Kristin] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27709 USA. [Gilbert, Mary; El-Masri, Hisham] US EPA, Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr Mail Code B105-03, Res Triangle Pk, NC 27709 USA. RP El-Masri, H (reprint author), US EPA, Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr Mail Code B105-03, Res Triangle Pk, NC 27709 USA. EM el-masri.hisham@epa.gov FU Oak Ridge Institute for Science and Education Research Participation Program at the U.S. EPA FX Jeremy Leonard was funded through the Oak Ridge Institute for Science and Education Research Participation Program at the U.S. EPA. NR 62 TC 0 Z9 0 U1 6 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD MAY PY 2016 VL 151 IS 1 BP 57 EP 70 DI 10.1093/toxsci/kfw022 PG 14 WC Toxicology SC Toxicology GA DM9CU UT WOS:000376662800006 PM 26865668 ER PT J AU Ryan, N Chorley, B Tice, RR Judson, R Corton, JC AF Ryan, Natalia Chorley, Brian Tice, Raymond R. Judson, Richard Corton, J. Christopher TI Moving Toward Integrating Gene Expression Profiling Into High-Throughput Testing: A Gene Expression Biomarker Accurately Predicts Estrogen Receptor alpha Modulation in a Microarray Compendium SO TOXICOLOGICAL SCIENCES LA English DT Article DE estrogen receptor; gene expression profiling; MCF-7 cell line; biomarker ID BREAST-CANCER-CELLS; ENDOCRINE-DISRUPTING CHEMICALS; CHIP-SEQ DATA; IN-VITRO; CONNECTIVITY MAP; PROTEIN; TRANSCRIPTION; ACTIVATION; ESTRADIOL; PATHWAYS AB Microarray profiling of chemical-induced effects is being increasingly used in medium- and high-throughput formats. Computational methods are described here to identify molecular targets from whole-genome microarray data using as an example the estrogen receptor alpha (ER alpha), often modulated by potential endocrine disrupting chemicals. ER alpha biomarker genes were identified by their consistent expression after exposure to 7 structurally diverse ER alpha agonists and 3 ER alpha antagonists in ER alpha-positive MCF-7 cells. Most of the biomarker genes were shown to be directly regulated by ER alpha as determined by ESR1 gene knockdown using siRNA as well as through chromatin immunoprecipitation coupled with DNA sequencing analysis of ER alpha-DNA interactions. The biomarker was evaluated as a predictive tool using the fold-change rank-based Running Fisher algorithm by comparison to annotated gene expression datasets from experiments using MCF-7 cells, including those evaluating the transcriptional effects of hormones and chemicals. Using 141 comparisons from chemical- and hormone-treated cells, the biomarker gave a balanced accuracy for prediction of ER alpha activation or suppression of 94% and 93%, respectively. The biomarker was able to correctly classify 18 out of 21 (86%) ER reference chemicals including "very weak" agonists. Importantly, the biomarker predictions accurately replicated predictions based on 18 in vitro high-throughput screening assays that queried different steps in ER alpha signaling. For 114 chemicals, the balanced accuracies were 95% and 98% for activation or suppression, respectively. These results demonstrate that the ER alpha gene expression biomarker can accurately identify ER alpha modulators in large collections of microarray data derived from MCF-7 cells. C1 [Ryan, Natalia] US EPA, ORISE, Res Triangle Pk, NC 27711 USA. [Ryan, Natalia; Chorley, Brian; Corton, J. Christopher] US EPA, Integrated Syst Toxicol Div, Res Triangle Pk, NC 27711 USA. [Tice, Raymond R.] US EPA, NIEHS, Div Natl Toxicol Program, Res Triangle Pk, NC 27711 USA. [Judson, Richard] US EPA, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA. RP Corton, JC (reprint author), US EPA, Natl Hlth & Environm Effects Res Lab, Integrated Syst Toxicol Div, 109 TW Alexander Dr MD-B143-06, Res Triangle Pk, NC 27711 USA. EM corton.chris@epa.gov FU U.S. EPA; National Institute of Environmental Health Sciences FX The information in this document has been funded in part by the U.S. EPA and by the National Institute of Environmental Health Sciences. NR 59 TC 0 Z9 0 U1 2 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD MAY PY 2016 VL 151 IS 1 BP 88 EP 103 DI 10.1093/toxsci/kfw026 PG 16 WC Toxicology SC Toxicology GA DM9CU UT WOS:000376662800008 PM 26865669 ER PT J AU Friedman, KP Watt, ED Hornung, MW Hedge, JM Judson, RS Crofton, KM Houck, KA Simmons, SO AF Friedman, Katie Paul Watt, Eric D. Hornung, Michael W. Hedge, Joan M. Judson, Richard S. Crofton, Kevin M. Houck, Keith A. Simmons, Steven O. TI Tiered High-Throughput Screening Approach to Identify Thyroperoxidase Inhibitors Within the ToxCast Phase I and II Chemical Libraries SO TOXICOLOGICAL SCIENCES LA English DT Article DE endocrine; thyroid < endocrine toxicology in vitro and altenatives; predictive toxicology < in vitro and altenatives ID HORMONE-DISRUPTING CHEMICALS; MATERNAL THYROID-FUNCTION; EARLY-PREGNANCY; NEUROPSYCHOLOGICAL DEVELOPMENT; ENVIRONMENTAL CHEMICALS; HUMAN EXPOSURE; PEROXIDASE; HYPOTHYROXINEMIA; LACTOPEROXIDASE; METHIMAZOLE AB High-throughput screening for potential thyroid-disrupting chemicals requires a system of assays to capture multiple molecular-initiating events (MIEs) that converge on perturbed thyroid hormone (TH) homeostasis. Screening for MIEs specific to TH-disrupting pathways is limited in the U.S. Environmental Protection Agency ToxCast screening assay portfolio. To fill 1 critical screening gap, the Amplex UltraRed-thyroperoxidase (AUR-TPO) assay was developed to identify chemicals that inhibit TPO, as decreased TPO activity reduces TH synthesis. The ToxCast phase I and II chemical libraries, comprised of 1074 unique chemicals, were initially screened using a single, high concentration to identify potential TPO inhibitors. Chemicals positive in the single-concentration screen were retested in concentration-response. Due to high false-positive rates typically observed with loss-of-signal assays such as AUR-TPO, we also employed 2 additional assays in parallel to identify possible sources of nonspecific assay signal loss, enabling stratification of roughly 300 putative TPO inhibitors based upon selective AUR-TPO activity. A cell-free luciferase inhibition assay was used to identify nonspecific enzyme inhibition among the putative TPO inhibitors, and a cytotoxicity assay using a human cell line was used to estimate the cellular tolerance limit. Additionally, the TPO inhibition activities of 150 chemicals were compared between the AUR-TPO and an orthogonal peroxidase oxidation assay using guaiacol as a substrate to confirm the activity profiles of putative TPO inhibitors. This effort represents the most extensive TPO inhibition screening campaign to date and illustrates a tiered screening approach that focuses resources, maximizes assay throughput, and reduces animal use. C1 [Friedman, Katie Paul; Watt, Eric D.] Oak Ridge Inst Sci Educ Postdoctoral Fellow, Oak Ridge, TN 37831 USA. [Friedman, Katie Paul; Hedge, Joan M.] US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Watt, Eric D.; Judson, Richard S.; Crofton, Kevin M.; Houck, Keith A.; Simmons, Steven O.] US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Hornung, Michael W.] US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Duluth, MN 55804 USA. RP Simmons, SO (reprint author), US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Res Triangle Pk, NC 27711 USA. EM simmons.steve@epa.gov OI Watt, Eric/0000-0002-1211-0229 NR 59 TC 1 Z9 1 U1 10 U2 16 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD MAY PY 2016 VL 151 IS 1 BP 160 EP 180 DI 10.1093/toxsci/kfw034 PG 21 WC Toxicology SC Toxicology GA DM9CU UT WOS:000376662800014 ER PT J AU Pattinson, W Langstaff, J Longley, I Kingham, S AF Pattinson, Woodrow Langstaff, John Longley, Ian Kingham, Simon TI Use of an exposure model to explore the impact of residential proximity to a highway on exposures to air pollutants of an ambient origin SO AIR QUALITY ATMOSPHERE AND HEALTH LA English DT Article DE Exposure model; Near highway; Traffic emissions; Carbon monoxide; Nitrogen oxides ID PERSONAL EXPOSURE; POLLUTION EXPOSURE; CARDIOVASCULAR-DISEASE; ULTRAFINE PARTICLES; NEW-ZEALAND; URBAN; OUTDOOR; INDOOR; HELSINKI; AUCKLAND AB Air pollutant exposure models are generally applied to large populations living across wide urban areas, and most do not account for daily variation in activity patterns, which can result in exposure misclassification. Far fewer studies exist where exposure is modeled for specific individuals using detailed time-activity data. We employed a novel application of the US-EPA's Air Pollution Exposure Model (APEX) to simulate exposure levels for 51 residents living within a small study area (1.5 km(2)) bisected by a heavily trafficked highway in South Auckland, New Zealand. The model produced daily exposure estimates of nitrogen oxides (NOx ), carbon monoxide (CO), and particulate matter (PM10) for the month of July, 2010. Inputs included pollutant and meteorological data monitored at sites positioned both upwind and downwind of the highway, as well as city monitoring sites north of the study area to represent work locations. A local resident survey provided time-activity diary input. The simulation was run once using the residents' home locations and four times with the population artificially placed 50 and 150 m downwind, as well as 50 and 150 m upwind, relative to the highway. For NO (x) and CO, the population was 31-36 % more exposed when positioned 50 m downwind and 17-18 % less exposed at the upwind side (p < 0.001), compared to their actual home locations. An additional 100 m separation downwind resulted in a 56-71 % drop in total mean exposure (p < 0.001) and the difference in exposure levels for certain occupations varied by up to a factor of eight (p < 0.05). PM10 exposure was comparatively stable across the area. The effect of residential proximity and position, occupation and work location, were assessed using generalized linear models (GLMs), followed by post hoc testing. This unique application of APEX shows good promise as a planning tool for assessing the potential benefits of a buffer zone between major roads and residential homes, for particular population groups. C1 [Pattinson, Woodrow; Kingham, Simon] Univ Canterbury, Dept Geog, Private Bag 4800, Christchurch 8020, New Zealand. [Langstaff, John] US EPA, Res Triangle Pk, NC 27711 USA. [Longley, Ian] Natl Inst Water & Atmospher Res, Auckland 1010, New Zealand. RP Pattinson, W (reprint author), Univ Canterbury, Dept Geog, Private Bag 4800, Christchurch 8020, New Zealand. EM woodrow.pattinson@mail.com OI Pattinson, Woodrow/0000-0002-9432-1483 FU National Institute of Water and Atmospheric Research (NIWA), under New Zealand Transport Agency (NZTA) [476 TAR09/18] FX This work was made possible as a result of research conducted by the National Institute of Water and Atmospheric Research (NIWA), under New Zealand Transport Agency (NZTA) funding - project 476 TAR09/18. We would also like to thank the Auckland Council for providing air quality data at the Penrose and Grafton sites. NR 50 TC 1 Z9 1 U1 4 U2 7 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 1873-9318 EI 1873-9326 J9 AIR QUAL ATMOS HLTH JI Air Qual. Atmos. Health PD MAY PY 2016 VL 9 IS 4 BP 335 EP 357 DI 10.1007/s11869-015-0343-6 PG 23 WC Environmental Sciences SC Environmental Sciences & Ecology GA DM0OY UT WOS:000376046500002 ER PT J AU Hagler, GSW Tang, W AF Hagler, Gayle S. W. Tang, Wei TI Simulation of rail yard emissions transport to the near-source environment SO ATMOSPHERIC POLLUTION RESEARCH LA English DT Article DE Rail yard; Air pollution; Near-source; CFD; Dispersion ID AIR-QUALITY; MODEL; DISPERSION; IMPACTS; ATLANTA AB Rail yards are important nodes in the freight transportation network. However, they are also a focus of public health concern when located in highly populated areas. Field characterization of near-rail yard air quality is challenging due to spatially- and temporally-variable emissions. Numerical models can provide valuable insight into factors affecting emission dispersion and resulting near-field air pollution. This study utilizes computational fluid dynamics (CFD) modeling to investigate near-field air pollution surrounding a generic, moderate-sized intermodal rail yard with emissions of a neutrally buoyant gaseous pollutant. Rail yard and surrounding neighborhood structures were added in succession to a base case to study the influence of surface roughness on the generic pollutant's spatial concentration profile. A spatially weighted emissions scenario revealed highly variable pollutant levels in downwind neighborhoods, strongly affected by wind direction. Rail yard topography resulted in a modest increase in near-field pollution levels. Densely located two-story homes surrounding the rail yard reduced downwind concentrations by 16% and 15% at 25 m and 100 m downwind of the rail yard boundary, respectively. A 6 m boundary wall surrounding the rail yard, with four open sections enabling traffic flow, reduced downwind pollution levels by 25% and 12% at 25 m and 100 m downwind, respectively. While average pollution levels were lower with the addition of neighborhoods and a surrounding boundary wall, high spatial variability in pollution levels resulted in some near-field areas experiencing increased pollution that are offset by reductions in other areas. Copyright (C) 2015 Turkish National Committee for Air Pollution Research and Control. Production and hosting by Elsevier B.V. All rights reserved. C1 [Hagler, Gayle S. W.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Tang, Wei] Lockheed Martin Corp, Informat Syst & Global Serv, Durham, NC USA. RP Hagler, GSW (reprint author), 109 TW Alexander Dr,Mail Code E343-02, Res Triangle Pk, NC 27711 USA. EM hagler.gayle@epa.gov FU U.S. Environmental Protection Agency FX This research was supported by the U.S. Environmental Protection Agency and implemented through the U.S. EPA Environmental Modeling and Visualization Laboratory (EMVL). The authors are appreciative of a number of EPA staff supporting this project, including Heidi Paulsen, Richard Shores, Robert Wright, David Heist, Steven Perry, and Jennifer Richmond-Bryant, as well as Lockheed Martin staff including Mike Uhl and Matt Freeman. NR 17 TC 0 Z9 0 U1 3 U2 8 PU TURKISH NATL COMMITTEE AIR POLLUTION RES & CONTROL-TUNCAP PI BUCA PA DOKUZ EYLUL UNIV, DEPT ENVIRONMENTAL ENGINEERING, TINAZTEPE CAMPUS, BUCA, IZMIR 35160, TURKEY SN 1309-1042 J9 ATMOS POLLUT RES JI Atmos. Pollut. Res. PD MAY PY 2016 VL 7 IS 3 BP 469 EP 476 DI 10.1016/j.apr.2015.11.003 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DM2LE UT WOS:000376177400010 ER PT J AU Vossler, T Cernikovsky, L Novak, J Williams, R AF Vossler, Teri Cernikovsky, Libor Novak, Jiri Williams, Ronald TI Source apportionment with uncertainty estimates of fine particulate matter in Ostrava, Czech Republic using Positive Matrix Factorization SO ATMOSPHERIC POLLUTION RESEARCH LA English DT Article DE Fine particulate matter; Czech Republic; Steel manufacturing; Home heating; Source apportionment ID POLYCYCLIC AROMATIC-HYDROCARBONS; COMBUSTION; QUALITY; DIESEL; AIR AB A 14-week investigation during a warm and cold seasons was conducted to improve understanding of air pollution sources that might be impacting air quality in Ostrava, the Czech Republic. Fine particulate matter (PM2.5) samples were collected in consecutive 12-h day and night increments during spring and fall 2012 sampling campaigns. Sampling sites were strategically located to evaluate conditions in close proximity of a large steel works industrial complex, as well as away from direct influence of the industrial complex. These samples were analyzed for metals and other elements, organic and elemental (black) carbon, and polycyclic aromatic hydrocarbons (PAHs). The PM2.5 samples were supplemented with pollutant gases and meteorological parameters. We applied the EPA PMF v5.1 model with uncertainty estimate features to the Ostrava data set. Using the model's bootstrapping procedure and other considerations, six factors were determined to provide the optimum solution. Each model run consisted of 100 iterations to ensure that the solution represents a global minimum. The resulting factors were identified as representing coal (power plants), mixed Cl, crustal, industrial 1 (alkali metals and PAHs), industrial 2 (transition metals), and home heat/transportation. The home heating source is thought to be largely domestic boilers burning low quality fuels such as lignite, wood, and domestic waste. Transportation-related combustion emissions could not be resolved as a separate factor. Uncertainty estimates support the general conclusion that the factors identified as representing coal power and home heat/transportation dominate the percent contribution to fine mass. Apportionment of regulated individual species is also presented. Copyright (C) 2015 Turkish National Committee for Air Pollution Research and Control. Production and hosting by Elsevier B.V. All rights reserved. C1 [Vossler, Teri; Williams, Ronald] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Cernikovsky, Libor; Novak, Jiri] Czech Hydrometeorol Inst, Prague, Czech Republic. RP Vossler, T (reprint author), US EPA, Mail Drop E205-03, Res Triangle Pk, NC 27711 USA. EM CONNER.TERI@EPA.GOV NR 24 TC 0 Z9 0 U1 11 U2 24 PU TURKISH NATL COMMITTEE AIR POLLUTION RES & CONTROL-TUNCAP PI BUCA PA DOKUZ EYLUL UNIV, DEPT ENVIRONMENTAL ENGINEERING, TINAZTEPE CAMPUS, BUCA, IZMIR 35160, TURKEY SN 1309-1042 J9 ATMOS POLLUT RES JI Atmos. Pollut. Res. PD MAY PY 2016 VL 7 IS 3 BP 503 EP 512 DI 10.1016/j.apr.2015.12.004 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DM2LE UT WOS:000376177400015 ER PT J AU Burnell, DK Cooper, J Xu, J Burden, DS AF Burnell, Daniel K. Cooper, Justin Xu, Jie Burden, David S. TI Graphical User Interface for AT123D-AT Solute Transport Model SO GROUNDWATER LA English DT Editorial Material C1 [Burnell, Daniel K.; Cooper, Justin] Tetra Tech Inc, 45610 Woodland Rd Suite 400, Sterling, VA 20166 USA. [Xu, Jie] Tetra Tech Inc, 17885 Karman Ave Suite 400, Irvine, CA 92614 USA. [Burden, David S.] US EPA, Natl Risk Management Res Lab, Ada, OK 74820 USA. RP Burnell, DK (reprint author), Tetra Tech Inc, 45610 Woodland Rd Suite 400, Sterling, VA 20166 USA. EM Dan.Burnell@TetraTech.com NR 3 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0017-467X EI 1745-6584 J9 GROUNDWATER JI Groundwater PD MAY-JUN PY 2016 VL 54 IS 3 BP 313 EP 314 DI 10.1111/gwat.12391 PG 2 WC Geosciences, Multidisciplinary; Water Resources SC Geology; Water Resources GA DM2WU UT WOS:000376209600002 PM 26713843 ER PT J AU Day-Lewis, FD Johnson, CD Slater, LD Robinson, JL Williams, JH Boyden, CL Werkema, D Lane, JW AF Day-Lewis, F. D. Johnson, C. D. Slater, L. D. Robinson, J. L. Williams, J. H. Boyden, C. L. Werkema, D. Lane, J. W. TI A Fractured Rock Geophysical Toolbox Method Selection Tool SO GROUNDWATER LA English DT Editorial Material AB Article impact statement: A spreadsheet-based software is presented to identify methods for use at fractured-rock sites, based on project goals and site description. C1 [Day-Lewis, F. D.; Johnson, C. D.; Boyden, C. L.; Lane, J. W.] US Geol Survey, Branch Geophys, Off Groundwater, 11 Sherman Pl,Unit 5015, Storrs, CT 06269 USA. [Slater, L. D.; Robinson, J. L.] Rutgers State Univ, Dept Earth & Environm Sci, Newark, NJ 07102 USA. [Williams, J. H.] US Geol Survey, New York Water Sci Ctr, Troy, NY 12180 USA. [Werkema, D.] US EPA, Div Environm Sci, Characterizat & Monitoring Branch, Off Res & Dev, Las Vegas, NV 89119 USA. RP Day-Lewis, FD (reprint author), US Geol Survey, Branch Geophys, Off Groundwater, 11 Sherman Pl,Unit 5015, Storrs, CT 06269 USA. EM daylewis@usgs.gov OI Day-Lewis, Frederick/0000-0003-3526-886X NR 0 TC 1 Z9 1 U1 1 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0017-467X EI 1745-6584 J9 GROUNDWATER JI Groundwater PD MAY-JUN PY 2016 VL 54 IS 3 BP 315 EP 316 DI 10.1111/gwat.12397 PG 2 WC Geosciences, Multidisciplinary; Water Resources SC Geology; Water Resources GA DM2WU UT WOS:000376209600003 PM 26743439 ER PT J AU Koch, FW Voytek, EB Day-Lewis, FD Healy, R Briggs, MA Lane, JW Werkema, D AF Koch, Franklin W. Voytek, Emily B. Day-Lewis, Frederick D. Healy, Richard Briggs, Martin A. Lane, John W., Jr. Werkema, Dale TI 1DTempPro V2: New Features for Inferring Groundwater/Surface-Water Exchange SO GROUNDWATER LA English DT Article ID TEMPERATURE TIME-SERIES; FLUX AB A new version of the computer program 1DTempPro extends the original code to include new capabilities for (1) automated parameter estimation, (2) layer heterogeneity, and (3) time-varying specific discharge. The code serves as an interface to the U.S. Geological Survey model VS2DH and supports analysis of vertical one-dimensional temperature profiles under saturated flow conditions to assess groundwater/surface-water exchange and estimate hydraulic conductivity for cases where hydraulic head is known. C1 [Koch, Franklin W.] US Geol Survey, Denver, CO 80225 USA. [Koch, Franklin W.] Univ Calgary, Dept Geosci, ES118 2500 Univ Dr NW, Calgary, AB T2N 1N4, Canada. [Voytek, Emily B.; Day-Lewis, Frederick D.; Briggs, Martin A.; Lane, John W., Jr.] US Geol Survey, Off Groundwater, 11 Sherman Pl,Unit 5015, Storrs, CT 06269 USA. [Day-Lewis, Frederick D.] US Geol Survey, Branch Geophys, Off Groundwater, Denver Fed Ctr, POB 25046,MS 418,Bldng 53, Denver, CO 80225 USA. [Healy, Richard] US Geol Survey, Denver Fed Ctr, POB 25046,MS 418,Bldng 53, Denver, CO 80225 USA. [Werkema, Dale] US EPA, Div Environm Sci, Characterizat & Monitoring Branch, 944 E Harmon Ave, Las Vegas, NV 89119 USA. RP Day-Lewis, FD (reprint author), US Geol Survey, Branch Geophys, Off Groundwater, Denver Fed Ctr, POB 25046,MS 418,Bldng 53, Denver, CO 80225 USA. EM daylewis@usgs.gov OI Day-Lewis, Frederick/0000-0003-3526-886X FU USGS Toxic Substances Hydrology and Groundwater Resources Programs; United States Environmental Protection Agency through its Office of Research and Development [EP10D000782] FX The authors are grateful for useful comments from Timothy McCobb (USGS). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. The USGS Toxic Substances Hydrology and Groundwater Resources Programs supported this work. The United States Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here under contract EP10D000782. This paper has been subjected to Agency review and approved for publication. NR 19 TC 3 Z9 3 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0017-467X EI 1745-6584 J9 GROUNDWATER JI Groundwater PD MAY-JUN PY 2016 VL 54 IS 3 BP 434 EP 439 DI 10.1111/gwat.12369 PG 6 WC Geosciences, Multidisciplinary; Water Resources SC Geology; Water Resources GA DM2WU UT WOS:000376209600016 PM 26372016 ER PT J AU Omernik, J Paulsen, S Griffith, G Weber, M AF Omernik, J. Paulsen, S. Griffith, G. Weber, M. TI Regional patterns of total nitrogen concentrations in the National Rivers and Streams Assessment SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE ecoregions; environmental sampling; nitrogen; nutrients; water quality; watersheds ID CONTERMINOUS UNITED-STATES; REACTIVE NITROGEN; LAND; ECOREGIONS; WATERSHEDS; POULTRY AB Patterns of nitrogen (N) concentrations in streams sampled by the National Rivers and Streams Assessment (NRSA) were examined semiquantitatively to identify regional differences in stream N levels. The data were categorized and analyzed by watershed size classes to reveal patterns of the concentrations that are consistent with the spatial homogeneity in natural and anthropogenic characteristics associated with regional differences in N levels. Ecoregions and mapped information on human activities including agricultural practices were used to determine the resultant regions. Marked differences in N levels were found among the nine aggregations of ecoregions used to report the results of the NRSA. We identified distinct regional patterns of stream N concentrations within the reporting regions that are associated with the characteristics of specific Level III ecoregions, groups of Level III ecoregions, groups of Level IV ecoregions, certain geographic characteristics within ecoregions, and/or particular watershed size classes. We described each of these regions and illustrated their areal extent and median and range in N concentrations. Understanding the spatial variability of nutrient concentrations in flowing waters and the apparent contributions that human and nonhuman factors have on different sizes of streams and rivers is critical to the development of effective water quality assessment and management plans. This semi quantitative analysis is also intended to identify areas within which more detailed quantitative work can be conducted to determine specific regional factors associated with variations in stream N concentrations. C1 [Omernik, J.; Griffith, G.] US Geol Survey, Corvallis, OR USA. [Paulsen, S.; Weber, M.] US EPA, Corvallis, OR USA. RP Omernik, J (reprint author), US Geol Survey, Corvallis, OR USA. OI Weber, Marc/0000-0002-9742-4744 NR 47 TC 1 Z9 1 U1 4 U2 14 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2016 VL 71 IS 3 BP 167 EP 181 DI 10.2489/jswc.71.3.167 PG 15 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DM3BC UT WOS:000376220800005 ER PT J AU Shanks, OC Kelty, CA Oshiro, R Haugland, RA Madi, T Brooks, L Field, KG Sivaganesan, M AF Shanks, Orin C. Kelty, Catherine A. Oshiro, Robin Haugland, Richard A. Madi, Tania Brooks, Lauren Field, Katharine G. Sivaganesan, Mano TI Data Acceptance Criteria for Standardized Human-Associated Fecal Source Identification Quantitative Real-Time PCR Methods SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID MICROBIAL SOURCE TRACKING; 16S RIBOSOMAL-RNA; SURFACE-WATER SAMPLES; GENETIC-MARKERS; INDICATOR BACTERIA; IDENTIFY SOURCES; POLLUTION; ASSAYS; BACTEROIDALES; CONTAMINATION AB There is growing interest in the application of human-associated fecal source identification quantitative real-time PCR (qPCR) technologies for water quality management. The transition from a research tool to a standardized protocol requires a high degree of confidence in data quality across laboratories. Data quality is typically determined through a series of specifications that ensure good experimental practice and the absence of bias in the results due to DNA isolation and amplification interferences. However, there is currently a lack of consensus on how best to evaluate and interpret human fecal source identification qPCR experiments. This is, in part, due to the lack of standardized protocols and information on interlaboratory variability under conditions for data acceptance. The aim of this study is to provide users and reviewers with a complete series of conditions for data acceptance derived from a multiple laboratory data set using standardized procedures. To establish these benchmarks, data from HF183/BacR287 and HumM2 human-associated qPCR methods were generated across 14 laboratories. Each laboratory followed a standardized protocol utilizing the same lot of reference DNA materials, DNA isolation kits, amplification reagents, and test samples to generate comparable data. After removal of outliers, a nested analysis of variance (ANOVA) was used to establish proficiency metrics that include lab-to-lab, replicate testing within a lab, and random error for amplification inhibition and sample processing controls. Other data acceptance measurements included extraneous DNA contamination assessments (no-template and extraction blank controls) and calibration model performance (correlation coefficient, amplification efficiency, and lower limit of quantification). To demonstrate the implementation of the proposed standardized protocols and data acceptance criteria, comparable data from two additional laboratories were reviewed. The data acceptance criteria proposed in this study should help scientists, managers, reviewers, and the public evaluate the technical quality of future findings against an established benchmark. C1 [Shanks, Orin C.; Kelty, Catherine A.; Haugland, Richard A.; Sivaganesan, Mano] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. [Oshiro, Robin] US EPA, Off Water, Washington, DC 20460 USA. [Madi, Tania] Source Mol Corp, Miami, FL USA. [Brooks, Lauren; Field, Katharine G.] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA. RP Shanks, OC (reprint author), US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. EM shanks.orin@epa.gov NR 50 TC 3 Z9 3 U1 12 U2 30 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAY PY 2016 VL 82 IS 9 BP 2773 EP 2782 DI 10.1128/AEM.03661-15 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DK0ZA UT WOS:000374640400020 PM 26921430 ER PT J AU Soto-Giron, MJ Rodriguez-R, LM Luo, CW Elk, M Ryu, H Hoelle, J Domingo, JWS Konstantinidis, KT AF Soto-Giron, Maria J. Rodriguez-R, Luis M. Luo, Chengwei Elk, Michael Ryu, Hodon Hoelle, Jill Domingo, Jorge W. Santo Konstantinidis, Konstantinos T. TI Biofilms on Hospital Shower Hoses: Characterization and Implications for Nosocomial Infections SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID SPHINGOMONAS-PAUCIMOBILIS BACTEREMIA; ANTIBIOTIC-RESISTANCE GENES; WATER DISTRIBUTION-SYSTEMS; DRINKING-WATER; NONTUBERCULOUS MYCOBACTERIA; METAGENOMIC SEQUENCES; BACTERIAL COMMUNITIES; TAP WATER; GENOME; DATABASE AB Although the source of drinking water (DW) used in hospitals is commonly disinfected, biofilms forming on water pipelines are a refuge for bacteria, including possible pathogens that survive different disinfection strategies. These biofilm communities are only beginning to be explored by culture-independent techniques that circumvent the limitations of conventional monitoring efforts. Hence, theories regarding the frequency of opportunistic pathogens in DW biofilms and how biofilm members withstand high doses of disinfectants and/or chlorine residuals in the water supply remain speculative. The aim of this study was to characterize the composition of microbial communities growing on five hospital shower hoses using both 16S rRNA gene sequencing of bacterial isolates and whole-genome shotgun metagenome sequencing. The resulting data revealed a Mycobacterium-like population, closely related to Mycobacterium rhodesiae and Mycobacterium tusciae, to be the predominant taxon in all five samples, and its nearly complete draft genome sequence was recovered. In contrast, the fraction recovered by culture was mostly affiliated with Proteobacteria, including members of the genera Sphingomonas, Blastomonas, and Porphyrobacter. The biofilm community harbored genes related to disinfectant tolerance (2.34% of the total annotated proteins) and a lower abundance of virulence determinants related to colonization and evasion of the host immune system. Additionally, genes potentially conferring resistance to beta-lactam, aminoglycoside, amphenicol, and quinolone antibiotics were detected. Collectively, our results underscore the need to understand the microbiome of DW biofilms using metagenomic approaches. This information might lead to more robust management practices that minimize the risks associated with exposure to opportunistic pathogens in hospitals. C1 [Soto-Giron, Maria J.; Rodriguez-R, Luis M.; Konstantinidis, Konstantinos T.] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA. [Soto-Giron, Maria J.; Rodriguez-R, Luis M.; Luo, Chengwei; Konstantinidis, Konstantinos T.] Georgia Inst Technol, Ctr Bioinformat & Computat Genom, Atlanta, GA 30332 USA. [Luo, Chengwei; Konstantinidis, Konstantinos T.] Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA. [Elk, Michael] Pegasus Inc, Cincinnati, OH USA. [Ryu, Hodon; Hoelle, Jill; Domingo, Jorge W. Santo] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. RP Konstantinidis, KT (reprint author), Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA.; Konstantinidis, KT (reprint author), Georgia Inst Technol, Ctr Bioinformat & Computat Genom, Atlanta, GA 30332 USA.; Konstantinidis, KT (reprint author), Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA.; Domingo, JWS (reprint author), US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. EM santodomingo.jorge@epamail.epa.gov; kostas@ce.gatech.edu OI Rodriguez-R, Luis M/0000-0001-7603-3093 FU National Research Council (EPA/NRC); Departamento Administrativo de Ciencia, Tecnologia e Innovacion (COLCIENCIAS) [529] FX This work, including the efforts of Hodon Ryu, was funded by National Research Council (EPA/NRC-2013). This work, including the efforts of Maria J. Soto-Giron, was funded by Departamento Administrativo de Ciencia, Tecnologia e Innovacion (COLCIENCIAS) (529). NR 92 TC 3 Z9 3 U1 8 U2 25 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAY PY 2016 VL 82 IS 9 BP 2872 EP 2883 DI 10.1128/AEM.03529-15 PG 12 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DK0ZA UT WOS:000374640400030 PM 26969701 ER PT J AU Dewi, FN Wood, CE Willson, CJ Register, TC Lees, CJ Howard, TD Huang, ZQ Murphy, SK Tooze, JA Chou, JW Miller, LD Cline, JM AF Dewi, Fitriya N. Wood, Charles E. Willson, Cynthia J. Register, Thomas C. Lees, Cynthia J. Howard, Timothy D. Huang, Zhiqing Murphy, Susan K. Tooze, Janet A. Chou, Jeff W. Miller, Lance D. Cline, J. Mark TI Effects of Pubertal Exposure to Dietary Soy on Estrogen Receptor Activity in the Breast of Cynomolgus Macaques SO CANCER PREVENTION RESEARCH LA English DT Article ID MAMMARY-GLAND; NONHUMAN-PRIMATES; EPITHELIAL-CELLS; CANCER RISK; FOOD-INTAKE; METHYLATION; EXPRESSION; GENISTEIN; ALPHA; GENE AB Endogenous estrogens influence mammary gland development during puberty and breast cancer risk during adulthood. Early-life exposure to dietary or environmental estrogens may alter estrogen-mediated processes. Soy foods contain phytoestrogenic isoflavones (IF), which have mixed estrogen agonist/antagonist properties. Here, we evaluated mammary gland responses over time in pubertal female cynomolgus macaques fed diets containing either casein/lactalbumin (n = 12) or soy protein containing a human-equivalent dose of 120 mg IF/day (n = 17) for approximately 4.5 years spanning menarche. We assessed estrogen receptor (ER) expression and activity, promoter methylation of ERs and their downstream targets, and markers of estrogen metabolism. Expression of ER alpha and classical ER alpha response genes (TFF1, PGR, and GREB1) decreased with maturity, independent of diet. A significant inverse correlation was observed between TFF1 mRNA and methylation of CpG sites within the TFF1 promoter. Soy effects included lower ER beta expression before menarche and lower mRNA for ER alpha and GREB1 after menarche. Expression of GATA-3, an epithelial differentiation marker that regulates ER alpha-mediated transcription, was elevated before menarche and decreased after menarche in soy-fed animals. Soy did not significantly alter expression of other ER activity markers, estrogen-metabolizing enzymes, or promoter methylation for ERs or ER-regulated genes. Our results demonstrate greater ER expression and activity during the pubertal transition, supporting the idea that this life stage is a critical window for phenotypic modulation by estrogenic compounds. Pubertal soy exposure decreases mammary ER alpha expression after menarche and exerts subtle effects on receptor activity and mammary gland differentiation. (C) 2016 AACR. C1 [Dewi, Fitriya N.; Wood, Charles E.; Willson, Cynthia J.; Register, Thomas C.; Lees, Cynthia J.; Cline, J. Mark] Wake Forest Sch Med, Comparat Med Sect, Dept Pathol, Winston Salem, NC USA. [Dewi, Fitriya N.] Bogor Agr Univ, Primate Res Ctr, Jl Lodaya 2 5, Bogor 16151, Indonesia. [Howard, Timothy D.] Wake Forest Sch Med, Ctr Genom & Personalized Med Res, Winston Salem, NC USA. [Huang, Zhiqing; Murphy, Susan K.] Duke Univ, Sch Med, Dept Obstet & Gynecol, Div Gynecol Oncol, Durham, NC USA. [Tooze, Janet A.; Chou, Jeff W.] Wake Forest Sch Med, Dept Biostat Sci, Winston Salem, NC USA. [Miller, Lance D.] Wake Forest Sch Med, Dept Canc Biol, Winston Salem, NC USA. [Wood, Charles E.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Willson, Cynthia J.] Integrated Lab Syst Inc, Res Triangle Pk, NC USA. RP Dewi, FN (reprint author), Bogor Agr Univ, Primate Res Ctr, Jl Lodaya 2 5, Bogor 16151, Indonesia. EM fitriya.dewi@gmail.com FU NIH [R01 AT000639, T32 OD010957]; NCI CCSG [P30CA012197] FX This work was supported by NIH R01 AT000639 (to J.M. Cline) and T32 OD010957 (to J.M. Cline and C.J. Willson). The Comprehensive Cancer Center of Wake Forest University Microarray Shared Resource is supported by NCI CCSG P30CA012197 (to B.C. Pasche). NR 50 TC 0 Z9 0 U1 2 U2 5 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1940-6207 EI 1940-6215 J9 CANCER PREV RES JI Cancer Prev. Res. PD MAY PY 2016 VL 9 IS 5 BP 385 EP 395 DI 10.1158/1940-6207.CAPR-15-0165 PG 11 WC Oncology SC Oncology GA DL7VR UT WOS:000375848700007 PM 27006379 ER PT J AU Forbes, V Hall, T Suter, GW Calow, P AF Forbes, Valery Hall, Tilghman Suter, Glenn W. Calow, Peter TI In Response: We are all biased, but the scientific process recognizes that and delivers despite it; still, it can do a better job-A perspective from academia SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Editorial Material C1 [Forbes, Valery; Calow, Peter] Univ Minnesota, St Paul, MN 55108 USA. [Hall, Tilghman] Bayer CropSci, Monheim, Germany. [Suter, Glenn W.] US EPA, Cincinnati, OH 45268 USA. RP Forbes, V (reprint author), Univ Minnesota, St Paul, MN 55108 USA. EM veforbes@umn.edu OI Forbes, Valery/0000-0001-9819-9385 NR 8 TC 2 Z9 2 U1 0 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD MAY PY 2016 VL 35 IS 5 BP 1068 EP 1069 DI 10.1002/etc.3355 PG 2 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DJ9QH UT WOS:000374547500002 PM 27089439 ER PT J AU Suter, G Cormier, S AF Suter, Glenn Cormier, Susan TI In Response: Bias in the science that supports environmental assessments-A perspective from regulatory assessment SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Editorial Material ID HEALTH C1 [Suter, Glenn; Cormier, Susan] US EPA, Natl Ctr Environm Assessment, Cincinnati, OH 45268 USA. RP Suter, G (reprint author), US EPA, Natl Ctr Environm Assessment, Cincinnati, OH 45268 USA. NR 12 TC 0 Z9 0 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD MAY PY 2016 VL 35 IS 5 BP 1069 EP 1070 DI 10.1002/etc.3356 PG 2 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DJ9QH UT WOS:000374547500003 PM 27089440 ER PT J AU Hudepohl, MR Buchberger, SG Shuster, WD AF Hudepohl, Michael R. Buchberger, Steven G. Shuster, William D. TI Hydrology of Synthetic Turf Fields: Modeling Approach with Field Data SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Article DE Synthetic turf fields; Rational method; HYDRUS; Storm Water Management Model (SWMM) ID WATER SYSTEMS AB The hydrology of synthetic turf field (STF) drainage systems is poorly understood, which complicates their design. Field data are collected and used to parameterize computer models simulating the hydrology of a synthetic turf sport field. A model was employed to simulate infiltration through the synthetic turf, into the subsoil matrix, and free drainage into the perforated underdrains. The output from that model was routed using another model to simulate flow through the subsurface pipe drainage network to the system outfall. The drainage model was calibrated with actual hydraulic parameters obtained from field substrate material measurements, observed rainfall hyetographs, and measured discharge hydrographs for several storm events. Output from the calibrated STF drainage model closely matched the observed outlet hydrograph. Model output was then applied to investigate whether the classic rational formula was an appropriate tool for predicting peak flows from synthetic turf fields. It was determined that a single unique C factor in the rational framework could not adequately account for the more complex rainfall-runoff response of the synthetic turf drainage system. The implications of this new hydrologic model of STF performance are discussed. (C) 2016 American Society of Civil Engineers. C1 [Hudepohl, Michael R.] Univ Cincinnati, Environm Engn Program, Cincinnati, OH 45221 USA. [Buchberger, Steven G.] Univ Cincinnati, Dept Civil & Environm Engn, 765 Baldwin ML71, Cincinnati, OH 45221 USA. [Shuster, William D.] US EPA, Natl Risk Management Res Lab, Off Res & Dev, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. RP Shuster, WD (reprint author), US EPA, Natl Risk Management Res Lab, Off Res & Dev, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM hudepoml@mail.uc.edu; steven.buchberger@uc.edu; shuster.william@epa.gov FU University of Cincinnati FX The authors would like to thank Northern Kentucky University for field access, University of Cincinnati for financial aid, and The Kleingers Group for field plans and industry insights. NR 16 TC 0 Z9 0 U1 6 U2 12 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD MAY PY 2016 VL 142 IS 5 AR 04016006 DI 10.1061/(ASCE)IR.1943-4774.0000992 PG 9 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA DK2PX UT WOS:000374758000001 ER PT J AU Mukerjee, S Smith, LA Thoma, ED Oliver, KD Whitaker, DA Wu, T Colon, M Alston, L Cousett, TA Stallings, C AF Mukerjee, Shaibal Smith, Luther A. Thoma, Eben D. Oliver, Karen D. Whitaker, Donald A. Wu, Tai Colon, Maribel Alston, Lillian Cousett, Tamira A. Stallings, Casson TI Spatial analysis of volatile organic compounds in South Philadelphia using passive samplers SO JOURNAL OF THE AIR & WASTE MANAGEMENT ASSOCIATION LA English DT Article ID SIMULTANEOUS CONFIDENCE INTERVALS; CARBOPACK-X; MULTINOMIAL PROPORTIONS; DIFFUSIVE SAMPLERS; AIR AB Select volatile organic compounds (VOCs) were measured in the vicinity of a petroleum refinery and related operations in South Philadelphia, Pennsylvania, USA, using passive air sampling and laboratory analysis methods. Two-week, time-integrated samplers were deployed at 17 sites, which were aggregated into five site groups of varying distances from the refinery. Benzene, toluene, ethylbenzene, and xylene isomers (BTEX) and styrene concentrations were higher near the refinery's fenceline than for groups at the refinery's south edge, mid-distance, and farther removed locations. The near fenceline group was significantly higher than the refinery's north edge group for benzene and toluene but not for ethylbenzene or xylene isomers; styrene was lower at the near fenceline group versus the north edge group. For BTEX and styrene, the magnitude of estimated differences generally increased when proceeding through groups ever farther away from the petroleum refining. Perchloroethylene results were not suggestive of an influence from refining. These results suggest that emissions from the refinery complex contribute to higher concentrations of BTEX species and styrene in the vicinity of the plant, with this influence declining as distance from the petroleum refining increases.Implications: Passive sampling methodology for VOCs as discussed here is employed in recently enacted U.S. Environmental Protection Agency Methods 325A/B for determination of benzene concentrations at refinery fenceline locations. Spatial gradients of VOC concentration near the refinery fenceline were discerned in an area containing traffic and other VOC-related sources. Though limited, these findings can be useful in application of the method at such facilities to ascertain source influence. C1 [Mukerjee, Shaibal; Oliver, Karen D.; Whitaker, Donald A.; Colon, Maribel; Alston, Lillian] US EPA, Off Res & Dev, Natl Exposure Res Lab, E205-03, Res Triangle Pk, NC 27711 USA. [Smith, Luther A.; Cousett, Tamira A.; Stallings, Casson] Alion Sci & Technol, Durham, NC USA. [Thoma, Eben D.; Wu, Tai] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Alston, Lillian] Senior Environm Employment Program, Res Triangle Pk, NC USA. RP Mukerjee, S (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab, E205-03, Res Triangle Pk, NC 27711 USA. EM Mukerjee.Shaibal@epa.gov FU U.S. Environmental Protection Agency through its Office of Research and Development [EP-D-10-070, EP-D-11-006, EP-C-09-027] FX This study was an EPA Air Climate and Energy and Regional Applied Research Effort Project. The U.S. Environmental Protection Agency through its Office of Research and Development funded and managed the research described here under contracts EP-D-10-070, EP-D-11-006, and EP-C-09-027 to Alion Science and Technology, Eastern Research Group, and Arcadis, respectively. The paper has been subjected to Agency review and approved for publication. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. Mention of trade names or commercial products does not constitute an endorsement or recommendation for use. NR 14 TC 2 Z9 2 U1 5 U2 13 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1096-2247 EI 2162-2906 J9 J AIR WASTE MANAGE JI J. Air Waste Manage. Assoc. PD MAY PY 2016 VL 66 IS 5 BP 492 EP 498 DI 10.1080/10962247.2016.1147505 PG 7 WC Engineering, Environmental; Environmental Sciences; Meteorology & Atmospheric Sciences SC Engineering; Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DJ5EW UT WOS:000374230600006 PM 26828464 ER PT J AU Sundaree, S Vaddula, BR Tantak, MP Khandagale, SB Shi, C Shah, K Kumar, D AF Sundaree, Swapna Vaddula, Buchi Reddy Tantak, Mukund P. Khandagale, Santosh B. Shi, Chun Shah, Kavita Kumar, Dalip TI Synthesis and anticancer activity study of indolyl hydrazide-hydrazones SO MEDICINAL CHEMISTRY RESEARCH LA English DT Article DE Antiproliferative activity; Indole hydrazide-hydrazones; MTT assay ID POTENT CYTOTOXIC AGENTS; TUBULIN POLYMERIZATION; DERIVATIVES; ANALOGS; DESIGN; IDENTIFICATION; INHIBITION AB A series of N'-((1-(substituted)-1H-indol-3-yl)methylene)hydrazides were synthesized and evaluated for their in vitro antiproliferative activities against various cancer cell lines. Formation of indole hydrazide-hydrazones was accomplished by the reaction of indole 3-carboxaldehyde with aryl/alkyl hydrazides in the presence of acetic acid. Out of synthesized twenty-two compounds, some of the analogs exhibited specificity toward breast (18b, 18d, 18f and 18j) and prostate (18t and 18v) cancer cells. Among the prepared derivatives, compounds 18b, 18d and 18j were most cytotoxic (IC50 = 0.9, 0.4 and 0.8 A mu M, respectively) against the screened cancer cell lines. Exposure of PC3 cells to either 18d or 18j resulted in increased levels of cleaved PARP1, indicating that indolyl hydrazide-hydrazones induce apoptosis in PC3 cells. C1 [Sundaree, Swapna; Tantak, Mukund P.; Khandagale, Santosh B.; Kumar, Dalip] Birla Inst Technol & Sci, Dept Chem, Pilani 333031, Rajasthan, India. [Vaddula, Buchi Reddy] US EPA, Natl Risk Management Res Lab, Sustainable Technol Div, 26 West Martin Luther King Dr,MS 483, Cincinnati, OH 45268 USA. [Shi, Chun; Shah, Kavita] Purdue Univ, Dept Chem, 560 Oval Dr, W Lafayette, IN 47907 USA. [Shi, Chun; Shah, Kavita] Purdue Univ, Purdue Canc Ctr, 560 Oval Dr, W Lafayette, IN 47907 USA. RP Kumar, D (reprint author), Birla Inst Technol & Sci, Dept Chem, Pilani 333031, Rajasthan, India.; Shah, K (reprint author), Purdue Univ, Dept Chem, 560 Oval Dr, W Lafayette, IN 47907 USA.; Shah, K (reprint author), Purdue Univ, Purdue Canc Ctr, 560 Oval Dr, W Lafayette, IN 47907 USA. EM shah23@purdue.edu; dalipk@pilani.bits-pilani.ac.in FU CSIR; UGC, New Delhi FX We are grateful to CSIR and UGC, New Delhi, for financial support to perform the research work. This work was performed during the association of Swapna Sundaree and Buchi Reddy Vaddula with Birla Institute of Technology and Science, Pilani, India. NR 33 TC 1 Z9 1 U1 4 U2 6 PU SPRINGER BIRKHAUSER PI NEW YORK PA 233 SPRING STREET, 6TH FLOOR, NEW YORK, NY 10013 USA SN 1054-2523 EI 1554-8120 J9 MED CHEM RES JI Med. Chem. Res. PD MAY PY 2016 VL 25 IS 5 BP 941 EP 950 DI 10.1007/s00044-016-1522-1 PG 10 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA DJ4MO UT WOS:000374179900013 ER PT J AU Pachepsky, Y Shelton, D Dorner, S Whelan, G AF Pachepsky, Yakov Shelton, Daniel Dorner, Sarah Whelan, Gene TI Can E-coli or thermotolerant coliform concentrations predict pathogen presence or prevalence in irrigation waters? SO CRITICAL REVIEWS IN MICROBIOLOGY LA English DT Review DE Campylobacter; Cryptosporidium; food-borne illnesses; produce; Salmonella ID FECAL-INDICATOR CONCENTRATIONS; FRESH-WATER; UNITED-STATES; MICROBIAL INDICATORS; SURFACE WATERS; CAMPYLOBACTER-JEJUNI; LAND-USE; CRYPTOSPORIDIUM; GIARDIA; SALMONELLA AB An increase in food-borne illnesses in the United States has been associated with fresh produce consumption. Irrigation water presents recognized risks for microbial contamination of produce. Water quality criteria rely on indicator bacteria. The objective of this review was to collate and summarize experimental data on the relationships between pathogens and thermotolerant coliform (THT) and/or generic E. coli, specifically focusing on surface fresh waters used in or potentially suitable for irrigation agriculture. We analyzed peer-reviewed publications in which concentrations of E. coli or THT coliforms in surface fresh waters were measured along with concentrations of one or more of waterborne and food-borne pathogenic organisms. The proposed relationships were significant in 35% of all instances and not significant in 65% of instances. Coliform indicators alone cannot provide conclusive, non-site-specific and non-pathogen-specific information about the presence and/or concentrations of most important pathogens in surface waters suitable for irrigation. Standards of microbial water quality for irrigation can rely not only on concentrations of indicators and/or pathogens, but must include references to crop management. Critical information on microbial composition of actual irrigation waters to support criteria of microbiological quality of irrigation waters appears to be lacking and needs to be collected. C1 [Pachepsky, Yakov; Shelton, Daniel] USDA ARS, Environm Mirobial & Food Safety Lab, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. [Dorner, Sarah] Ecole Polytech, Dept Civil Geol & Min Engn, Montreal, PQ H3C 3A7, Canada. [Whelan, Gene] US EPA, Natl Exposure Res Lab, Athens, GA USA. RP Pachepsky, Y (reprint author), USDA ARS, Environm Mirobial & Food Safety Lab, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. EM yakov.pachepsky@ars.usda.gov OI Pachepsky, Yakov/0000-0003-0232-6090 FU United States Environmental Protection Agency through its Office of Research and Development [DW-12-92348101] FX The United States Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here under contract DW-12-92348101 to the USDA-ARS. It has been subjected to agency review and approved for publication. NR 78 TC 3 Z9 3 U1 9 U2 18 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1040-841X EI 1549-7828 J9 CRIT REV MICROBIOL JI Crit. Rev. Microbiol. PD MAY PY 2016 VL 42 IS 3 BP 384 EP 393 DI 10.3109/1040841X.2014.954524 PG 10 WC Microbiology SC Microbiology GA DK4OE UT WOS:000374897100004 PM 25198779 ER PT J AU Niehoff, NM Nichols, HB White, AJ Parks, CG D'Aloisio, AA Sandler, DP AF Niehoff, Nicole M. Nichols, Hazel B. White, Alexandra J. Parks, Christine G. D'Aloisio, Aimee A. Sandler, Dale P. TI Childhood and Adolescent Pesticide Exposure and Breast Cancer Risk SO EPIDEMIOLOGY LA English DT Article ID CURRENTLY USED PESTICIDES; RECEPTOR STATUS; EPIDEMIOLOGIC EVIDENCE; AGRICULTURAL HEALTH; BODY BURDEN; ESTROGEN; ORGANOCHLORINES; RELIABILITY; WOMEN; LIFE AB Background: To date, epidemiologic studies have not strongly supported an association between pesticide exposure and breast cancer. However, few previous studies had the ability to assess specific time periods of exposure. Studies that relied on adult serum levels of metabolites of organochlorine pesticides may not accurately reflect exposure during developmental periods. Furthermore, exposure assessment often occurred after diagnosis and key tumor characteristics, such as hormone receptor status, have rarely been available to evaluate tumor subtype-specific associations. We examined the association between pesticide exposure during childhood and adolescence and breast cancer risk in the prospective Sister Study cohort (N = 50,884 women) to assess this relation by tumor subtype. Methods: During an average 5-year follow-up, 2,134 incident invasive and in situ breast cancer diagnoses were identified. Residential and farm exposure to pesticides were self-reported at study enrollment during standardized interviews. Multivariable hazard ratios and 95% confidence intervals for breast cancer risk were calculated with Cox proportional hazards regression. Results: HRs were near null for the association between childhood/adolescent pesticide exposure and breast cancer risk overall or among ER+/PR+ invasive tumors. However, among women who were ages 0-18 before the ban of dichlordiphenyltrichloroethane in the US, exposure to fogger trucks or planes was associated with a hazard ratio = 1.3 for premenopausal breast cancer (95% confidence interval: 0.92, 1.7). Conclusion: These findings do not support an overall association between childhood and adolescent pesticide exposure and breast cancer risk. However, modest increases in breast cancer risk were associated with acute events in a subgroup of young women. C1 [Niehoff, Nicole M.; Nichols, Hazel B.; White, Alexandra J.] Univ N Carolina, Dept Epidemiol, Gillings Sch Global Publ Hlth, 2102A McGavran Greenberg Hall,135 Dauer Dr, Chapel Hill, NC 27516 USA. [Parks, Christine G.; Sandler, Dale P.] Natl Inst Environm Hlth Sci, Epidemiol Branch, Res Triangle Pk, NC USA. [D'Aloisio, Aimee A.] Social & Sci Syst Inc, Durham, NC USA. RP Nichols, HB (reprint author), Univ N Carolina, Dept Epidemiol, Gillings Sch Global Publ Hlth, 2102A McGavran Greenberg Hall,135 Dauer Dr, Chapel Hill, NC 27516 USA. EM hazel.nichols@unc.edu OI Parks, Christine/0000-0002-5734-3456; Sandler, Dale/0000-0002-6776-0018 FU Intramural and Extramural Research Programs of the National Institutes of Health, National Institute of Environmental Health Sciences [Z01-ES044005, T32-ES007018]; National Center for Advancing Translational Sciences [KL2-TR001109]; UNC Lineberger Cancer Control Education Program [R25 CA57726] FX This research was supported in part by the Intramural and Extramural Research Programs of the National Institutes of Health, National Institute of Environmental Health Sciences (Z01-ES044005, T32-ES007018), by the National Center for Advancing Translational Sciences (KL2-TR001109), and by the UNC Lineberger Cancer Control Education Program (R25 CA57726). NR 44 TC 1 Z9 1 U1 4 U2 6 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1044-3983 EI 1531-5487 J9 EPIDEMIOLOGY JI Epidemiology PD MAY PY 2016 VL 27 IS 3 BP 326 EP 333 DI 10.1097/EDE.0000000000000451 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DK2XI UT WOS:000374777300020 PM 26808595 ER PT J AU Linker, LC Batiuk, RA Cerco, CF Shenk, GW Tian, R Wang, P Yactayo, G AF Linker, Lewis C. Batiuk, Richard A. Cerco, Carl F. Shenk, Gary W. Tian, Richard Wang, Ping Yactayo, Guido TI Influence of Reservoir Infill on Coastal Deep Water Hypoxia SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID CHESAPEAKE BAY; SEDIMENT LOAD; EUTROPHICATION; MODEL; TRENDS; BASIN AB Ecological restoration of the Chesapeake through the Chesapeake Bay total maximum daily load (TMDL) requires the reduction of nitrogen, phosphorus, and sediment loads in the Chesapeake watershed because of the tidal water quality impairments and damage to living resources they cause. Within the Chesapeake watershed, the Conowingo Reservoir has been filling in with sediment for almost a century and is now in a state of near-full capacity called dynamic equilibrium. The development of the Chesapeake TMDL in 2010 was with the assumption that the Conowingo Reservoir was still effectively trapping sediment and nutrients. This is now known not to be the case. In a TMDL, pollutant loads beyond the TMDL allocation, which are brought about by growth or other conditions, must be offset. Using the analysis tools of the Chesapeake TMDL for assessing the degree of water quality standard attainment, the estimated nutrient and sediment loads from a simulated dynamic equilibrium infill condition of the Conowingo Reservoir were determined. The influence on Chesapeake water quality by a large storm and scour event of January 1996 on the Susquehanna River was estimated, and the same storm and scour events were also evaluated in the more critical living resource period of June. An analysis was also made on the estimated influence of more moderate high flow events. The infill of the Conowingo reservoir had estimated impairments of water quality, primarily on deep-water and deep-channel dissolved oxygen, because of increased discharge and transport of organic and particulate inorganic nutrients from the Conowingo Reservoir. C1 [Linker, Lewis C.; Batiuk, Richard A.; Shenk, Gary W.] US EPA, Chesapeake Bay Program Off, 410 Severn Ave,Suite 109, Annapolis, MD 21403 USA. [Cerco, Carl F.] US Army, Engn Res & Dev Ctr, 3909 Halls Ferry Rd, Vicksburg, MS 39180 USA. [Tian, Richard; Yactayo, Guido] Univ Maryland, Ctr Environm Sci, Chesapeake Bay Program Off, 410 Severn Ave,Suite 109, Annapolis, MD 21403 USA. [Wang, Ping] Virginia Inst Marine Sci, Chesapeake Bay Off, 410 Severn Ave, Annapolis, MD 21403 USA. RP Linker, LC (reprint author), US EPA, Chesapeake Bay Program Off, 410 Severn Ave,Suite 109, Annapolis, MD 21403 USA. EM LLinker@chesapeakebay.net NR 38 TC 2 Z9 2 U1 3 U2 10 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 887 EP 893 DI 10.2134/jeq2014.11.0461 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700017 PM 27136155 ER PT J AU Park, Y Pachepsky, Y Shelton, D Jeong, J Whelan, G AF Park, Yongeun Pachepsky, Yakov Shelton, Daniel Jeong, Jaehak Whelan, Gene TI Survival of Manure-borne Escherichia coli and Fecal Coliforms in Soil: Temperature Dependence as Affected by Site-Specific Factors SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID AMENDED SOIL; LABORATORY CONDITIONS; INDICATOR ORGANISMS; PATHOGEN SURVIVAL; SEWAGE-SLUDGE; E. COLI; DIE-OFF; FATE; WATER; TRANSPORT AB Understanding pathogenic and indicator bacteria survival in soils is essential for assessing the potential of microbial contamination of water and produce. The objective of this work was to evaluate the effects of soil properties, animal source, experimental conditions, and the application method on temperature dependencies of manure-borne generic Escherichia coli, E. coli O157: H7, and fecal coliforms survival in soils. A literature search yielded 151 survival datasets from 70 publications. Either one-stage or two-stage kinetics was observed in the survival datasets. We used duration and rate of the logarithm of concentration change as parameters of the first stage in the two-stage kinetics data. The second stage of the two-stage kinetics and the one-stage kinetics were simulated with the Q(10) model to find the dependence of the inactivation rate on temperature. Classification and regression trees and linear regressions were applied to parameterize the kinetics. Presence or absence of two-stage kinetics was controlled by temperature, soil texture, soil water content, and for fine-textured soils by setting experiments in the field or in the laboratory. The duration of the first stage was predominantly affected by soil water content and temperature. In the Q(10) model dependencies of inactivation rates on temperature, parameter Q(10) estimates were significantly affected by the laboratory versus field conditions and by the application method, whereas inactivation rates at 20 degrees C were significantly affected by all survival and management factors. Results of this work can provide estimates of coliform survival parameters for models of microbial water quality. C1 [Park, Yongeun; Pachepsky, Yakov; Shelton, Daniel] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Jeong, Jaehak] Texas A&M Univ, Dept Biol & Agr Engn, Temple, TX 76504 USA. [Whelan, Gene] US EPA, Natl Exposure Res Lab, Ecosyst Res Div, Athens, GA 30605 USA. RP Pachepsky, Y (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM yakov.pachepsky@ars.usda.gov OI Park, Yongeun/0000-0002-1959-0843 FU USEPA through its Office of Research and Development [DW-12-92348101] FX The authors thank Gonzalo Martinez and Matthew Stocker for help with data collection. The USEPA through its Office of Research and Development partially funded and collaborated in the research described here under DW-12-92348101 to the USDA-ARS. It has been subjected to agency review and approved for publication. NR 68 TC 3 Z9 3 U1 9 U2 18 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 949 EP 957 DI 10.2134/jeq2015.08.0427 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700024 PM 27136162 ER PT J AU Phillips, CL Trippe, KM Whittaker, G Griffith, SM Johnson, MG Banowetz, GM AF Phillips, Claire L. Trippe, Kristin M. Whittaker, Gerald Griffith, Stephen M. Johnson, Mark G. Banowetz, Gary M. TI Gasified Grass and Wood Biochars Facilitate Plant Establishment in Acid Mine Soils SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID METAL RETENTION; HEAVY-METALS; REMEDIATION; AMENDMENTS; IMMOBILIZATION; GASIFICATION; FEEDSTOCKS; MECHANISMS; DRAINAGE; INCREASE AB Heavy metals in exposed mine tailings threaten ecosystems that surround thousands of abandoned mines in the United States. Biochars derived from the pyrolysis or gasification of biomass may serve as a valuable soil amendment to revegetate mine sites. We evaluated the ability of two biochars, produced by gasification of either Kentucky bluegrass seed screenings (KB) or mixed conifer wood (CW), to support the growth of plants in mine spoils from the abandoned Formosa and Almeda Mines in Oregon. To evaluate the potential for plant establishment in mine tailings, wheat was grown in tailings amended with biochar at rates ranging from 0 to 9% (w/w). Both KB and CW biochars promoted plant establishment by increasing soil pH, increasing concentrations of macro-and micronutrients, and decreasing the solubility and plant uptake of heavy metals. Formosa tailings required at least 4% biochar and Almeda soil required at least 2% biochar to promote healthy wheat growth. A complimentary experiment in which mine spoils were leached with simulated precipitation indicated that biochar amendment rates >= 4% were sufficient to neutralize the elution pH and reduce concentrations of potentially toxic elements (Zn, Cu, Ni, Al) to levels near or below concern. These findings support the use of gasified biochar amendments to revegetate acid mine soils. C1 [Phillips, Claire L.; Trippe, Kristin M.; Whittaker, Gerald; Griffith, Stephen M.; Banowetz, Gary M.] USDA ARS, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. [Johnson, Mark G.] US EPA, Natl Hlth & Environm Effects Res Lab, 200 SW 35th St, Corvallis, OR 97333 USA. RP Trippe, KM (reprint author), USDA ARS, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM Kristin.Trippe@ars.usda.gov FU USDA-ARS [2072-21410-004-00D]; USEPA FX Research in the laboratory of K.T. and J.W. is supported by USDA-ARS Project #2072-21410-004-00D. The information in this document has been funded in part by the USEPA. It has been subjected to review by the National Health and Environmental Effects Research Laboratory's Western Ecology Division and approved for publication. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the USDA, the ARS, or the USEPA of any product or service to the exclusion of others that may be suitable. NR 45 TC 1 Z9 1 U1 7 U2 11 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 1013 EP 1020 DI 10.2134/jeq2015.09.0470 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700031 PM 27136169 ER PT J AU Morrison, AC Gold, AJ Pelletier, MC AF Morrison, Alisa C. Gold, Arthur J. Pelletier, Marguerite C. TI Evaluating Key Watershed Components of Low Flow Regimes in New England Streams SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID ECOLOGICAL CONSEQUENCES; HYDROLOGIC ALTERATION; UNITED-STATES; ECOSYSTEMS; WETLANDS; HEALTH; RIVER; USA AB Water resource managers seeking to optimize stream ecosystem services and abstractions of water from watersheds need an understanding of the importance of land use, physical and climatic characteristics, and hydrography on different low flow components of stream hydrographs. Within 33 USGS gaged watersheds of southern New England, we assessed relationships between watershed variables and a set of low flow parameters by using an information-theoretical approach. The key variables identified by the Akaike Information Criteria (AIC) weighting factors as generating positive relationships with low flow events included percent stratified drift, mean elevation, drainage area, and mean August precipitation. The extent of wetlands in the watershed was negatively related to low flow magnitudes. Of the various land use variables, the percentage of developed land was found to have the highest importance and a negative relationship on low flow magnitudes, but was less important than wetlands and physical and climatic features. Our results suggest that management practices aimed to sustain low flows in fluvial systems can benefit from attention to specific watershed features. We draw attention to the finding that streams located in watersheds with high proportions of wetlands may require more stringent approaches to withdrawals to sustain fluvial ecosystems during drought periods, particularly in watersheds with extensive development and limited deposits of stratified drift. C1 [Morrison, Alisa C.; Gold, Arthur J.] Univ Rhode Isl, Dept Nat Resources Sci, One Greenhouse Rd, Kingston, RI 02881 USA. [Pelletier, Marguerite C.] US EPA, Off Res & Dev, Atlantic Ecol Div, Narragansett, RI 02882 USA. RP Gold, AJ (reprint author), Univ Rhode Isl, Dept Nat Resources Sci, One Greenhouse Rd, Kingston, RI 02881 USA. EM agold@uri.edu NR 48 TC 1 Z9 1 U1 5 U2 5 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 1021 EP 1028 DI 10.2134/jeq2015.08.0434 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700032 PM 27136170 ER PT J AU Black, RR Aurell, J Holder, A George, IJ Gullett, BK Hays, MD Geron, CD Tabor, D AF Black, Robert R. Aurell, Johanna Holder, Amara George, Ingrid J. Gullett, Brian K. Hays, Michael D. Geron, Chris D. Tabor, Dennis TI Characterization of gas and particle emissions from laboratory burns of peat SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Peat fires; PCDD/PCDF; PAHs; PM2.5; CO; Carbon ID MASS LABELED PCDD/PCDF; ORGANIC-MATTER LOST; PARTICULATE MATTER; NORTH-CAROLINA; TRACE GAS; BIOMASS; CARBON; COMBUSTION; FOREST; FUELS AB Peat cores collected from two locations in eastern North Carolina (NC, USA) were burned in a laboratory facility to characterize emissions during simulated field combustion. Particle and gas samples were analyzed to quantify emission factors for particulate matter (PM2.5), organic carbon (OC), elemental carbon, light absorbing carbon, absorption Angstrom exponent, metals, polycyclic aromatic hydrocarbons (PAHs), and polychlorinated dibenzodioxins/dibenzofurans (PCDDs/PCDFs). CO from the smoldering burns, up to 7 h in duration, contributed approximately 16% of the total carbon emitted. Emission factors for black carbon (BC) and light absorbing carbon (UVPM) were considerably lower than those found for forest litter burns. Emission factors for PCDDs/PCDFs were near published values for forest fuels, at 1-4 ng toxic equivalents (TEQ)/kg carbon burned (Cb). Total PAH concentrations of >= 12 mg/kg were higher than published data from biomass burns, but roughly the same in terms of toxicity. Application of these emission factors to the noteworthy 2008 "Evans Road" fire in NC indicates that PM2.5 and PCDD/PCDF emissions from this fire may have been 4-6% of the annual US inventory and 5% of the annual OC amount. Published by Elsevier Ltd. C1 [Black, Robert R.; Holder, Amara; George, Ingrid J.; Gullett, Brian K.; Hays, Michael D.; Geron, Chris D.; Tabor, Dennis] US EPA, Off Res & Dev, Natl Risk Management Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Black, Robert R.] US EPA, Oak Ridge Inst Sci Educ, Res Triangle Pk, NC 27711 USA. [Aurell, Johanna] Univ Dayton, Res Inst, 300 Coll Pk, Dayton, OH 45469 USA. RP Gullett, BK (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM gullett.brian@epa.gov FU U.S. Department of Energy [DW89922983]; EPA [DW89922983]; U.S. EPA; Joint Fire Sciences Program [08-1-3-03] FX This research was supported in part by Robert Black's appointment to the Research Participation Program for the U.S. Environmental Protection Agency, Office of Research and Development, administered by the Oak Ridge Institute for Science and Education through an interagency agreement (#DW89922983) between the U.S. Department of Energy and EPA. The research was supported primarily by the U.S. EPA; Chris Geron was supported in part by the Joint Fire Sciences Program, Project ID: 08-1-3-03. The authors appreciate the assistance of Vince Carter and Tom Crews (U.S. Fish and Wildlife Service) from the Pocosin Lakes and Alligator River National Wildlife Reserves. The authors gratefully acknowledge the assistance of Steve Tern, Mike Tufts, Dean Smith (ARCADIS U.S., Inc.), and Chris Pressley and Sue Kimbrough (U.S. EPA). The views expressed in this article are those of the author(s) and do not necessarily represent the views or policies of the U.S. EPA. NR 46 TC 2 Z9 2 U1 10 U2 33 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD MAY PY 2016 VL 132 BP 49 EP 57 DI 10.1016/j.atmosenv.2016.02.024 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DK0PK UT WOS:000374614500006 ER PT J AU George, IJ Black, RR Geron, CD Aurell, J Hays, MD Preston, WT Gullett, BK AF George, Ingrid J. Black, Robert R. Geron, Chris D. Aurell, Johanna Hays, Michael D. Preston, William T. Gullett, Brian K. TI Volatile and semivolatile organic compounds in laboratory peat fire emissions SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Biomass burning; Peat; Organic soil; Volatile organic compounds; Emission factors ID FINE-PARTICLE EMISSIONS; WOOD COMBUSTION; SOURCE APPORTIONMENT; PARTICULATE MATTER; NORTH-CAROLINA; AIR-QUALITY; GAS-PHASE; BIOMASS; TRACERS; FUELS AB In this study, volatile and semi-volatile organic compound (VOCs and SVOCs) mass emission factors were determined from laboratory peat fire experiments. The peat samples originated from two National Wildlife Refuges on the coastal plain of North Carolina, U.S.A. Gas- and particle-phase organic compounds were quantified by gas chromatography-mass spectrometry and by high pressure liquid chromatography. Hazardous air pollutants (HAPs) accounted for a large fraction (similar to 60%) of the speciated VOC emissions from peat burning, including large contributions of acetaldehyde, formaldehyde, benzene, toluene, and chloromethane. In the fine particle mass (PM2.5), the following organic compound classes were dominant: organic acids, levoglucosan, n-alkanes, and n-alkenes. Emission factors for the organic acids in PM2.5 including n-alkanoic acids, n-alkenoic acids, n-alkanedioic acids, and aromatic acids were reported for the first time for peat burning, representing the largest fraction of organic carbon (OC) mass (11-12%) of all speciated compound classes measured in this work. Levoglucosan contributed to 2-3% of the OC mass, while methoxyphenols represented 0.2-0.3% of the OC mass on a carbon mass basis. Retene was the most abundant particulate phase polycyclic aromatic hydrocarbon (PAH). Total HAP VOC and particulate PAH emissions from a 2008 peat wildfire in North Carolina were estimated, suggesting that peat fires can contribute a large fraction of state-wide HAP emissions. Published by Elsevier Ltd. C1 [George, Ingrid J.; Black, Robert R.; Geron, Chris D.; Hays, Michael D.; Gullett, Brian K.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Black, Robert R.] Oak Ridge Inst Sci Educ, Oak Ridge, TN USA. [Aurell, Johanna] Univ Dayton, Res Inst, 300 Coll Pk, Dayton, OH 45469 USA. [Preston, William T.] ARCADIS US Inc, Res Triangle Pk, NC 27711 USA. RP Gullett, BK (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM gullett.brian@epa.gov FU U.S. Department of Energy; EPA; Joint Fire Sciences Program FX This research was supported in part by Robert Black's appointment to the Research Participation Program for the U.S. Environmental Protection Agency, Office of Research and Development, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. Chris Geron was supported in part by the Joint Fire Sciences Program. The authors appreciate the assistance of Vince Carter and Tom Crews (U.S. Fish and Wildlife Service) from the Pocosin Lakes and Alligator River National Wildlife Reserves. The authors gratefully acknowledge the assistance of Steve Terll, Mike Tufts, and Dean Smith (ARCADIS U.S., Inc.), and John Walker, Dennis Tabor, Chris Pressley and Sue Kimbrough (U.S. EPA). NR 43 TC 2 Z9 3 U1 18 U2 36 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD MAY PY 2016 VL 132 BP 163 EP 170 DI 10.1016/j.atmosenv.2016.02.025 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DK0PK UT WOS:000374614500018 ER PT J AU Gan, CM Hogrefe, C Mathur, R Pleim, J Xing, J Wong, D Gilliam, R Pouliot, G Wei, C AF Gan, Chuen-Meei Hogrefe, Christian Mathur, Rohit Pleim, Jonathan Xing, Jia Wong, David Gilliam, Robert Pouliot, George Wei, Chao TI Assessment of the effects of horizontal grid resolution on long-term air quality trends using coupled WRF-CMAQ simulations SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Coupled WRF-CMAQ; Model; Grid resolution; Air quality trend; Computation cost ID UNITED-STATES; MODEL; RADIATION AB The objective of this study is to determine the adequacy of using a relatively coarse horizontal resolution (i.e. 36 km) to simulate long-term trends of pollutant concentrations and radiation variables with the coupled WRF-CMAQ model. WRF-CMAQ simulations over the continental United State are performed over the 2001 to 2010 time period at two different horizontal resolutions of 12 and 36 km. Both simulations used the same emission inventory and model configurations. Model results are compared both in space and time to assess the potential weaknesses and strengths of using coarse resolution in long-term air quality applications. The results show that the 36 km and 12 km simulations are comparable in terms of trends analysis for both pollutant concentrations and radiation variables. The advantage of using the coarser 36 km resolution is a significant reduction of computational cost, time and storage requirement which are key considerations when performing multiple years of simulations for trend analysis. However, if such simulations are to be used for local air quality analysis, finer horizontal resolution may be beneficial since it can provide information on local gradients. In particular, divergences between the two simulations are noticeable in urban, complex terrain and coastal regions. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Gan, Chuen-Meei; Hogrefe, Christian; Mathur, Rohit; Pleim, Jonathan; Xing, Jia; Wong, David; Gilliam, Robert; Pouliot, George] US EPA, Atmospher Modeling & Anal Div, NERL, ORD, Res Triangle Pk, NC 27711 USA. [Gan, Chuen-Meei] CSC Govt Solut LLC, Res Triangle Pk, NC 27709 USA. [Wei, Chao] Max Planck Inst Chem, Mainz, Germany. RP Gan, CM (reprint author), CSRA Co, Falls Church, VA USA. EM chuenmeei@gmail.com RI Pleim, Jonathan Pleim/C-1331-2017 OI Pleim, Jonathan Pleim/0000-0001-6190-6082 FU National Research Council Research Associateship Award; US Department of Energy [IA DE-SC0003782]; US Environmental Protection Agency [IA RW-89-9233260] FX This research was performed while Chuen-Meei Gan held a National Research Council Research Associateship Award at US EPA. The research presented in this study was supported through an interagency agreement between the US Department of Energy (funding IA DE-SC0003782) and the US Environmental Protection Agency (funding IA RW-89-9233260). It has been subject to the US EPA's administrative review and approved for publication. NR 10 TC 2 Z9 2 U1 10 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD MAY PY 2016 VL 132 BP 207 EP 216 DI 10.1016/j.atmosenv.2016.02.036 PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DK0PK UT WOS:000374614500022 ER PT J AU Mathur, MB Epel, E Kind, S Desai, M Parks, CG Sandler, DP Khazeni, N AF Mathur, Maya B. Epel, Elissa Kind, Shelley Desai, Manisha Parks, Christine G. Sandler, Dale P. Khazeni, Nayer TI Perceived stress and telomere length: A systematic review, meta-analysis, and methodologic considerations for advancing the field SO BRAIN BEHAVIOR AND IMMUNITY LA English DT Review DE Telomere; Stress; Cellular damage; Meta-analysis ID CORONARY-HEART-DISEASE; LIFE STRESS; PSYCHOLOGICAL STRESS; CARDIOVASCULAR-DISEASE; POSTMENOPAUSAL WOMEN; DEPRESSION; ASSOCIATION; CANCER; ADULTS; HEALTH AB Importance: Psychological stress contributes to numerous diseases and may do so in part through damage to telomeres, protective non-coding segments on the ends of chromosomes. Objective: We conducted a systematic review and meta-analysis to determine the association between self-reported, perceived psychological stress (PS) and telomere length (TL). Data sources: We searched 3 databases (PubMed, PsycInfo, and Scopus), completed manual searches of published and unpublished studies, and contacted all study authors to obtain potentially relevant data. Study selection: Two independent reviewers assessed studies for original research measuring (but not necessarily reporting the correlation between) PS and TL in human subjects. 23 studies met inclusion criteria; 22 (totaling 8948 subjects) could be meta-analyzed. Data extraction and synthesis: We assessed study quality using modified MINORS criteria. Since not all included studies reported PS-TL correlations, we obtained them via direct calculation from author provided data (7 studies), contact with authors (14 studies), or extraction from the published article (1 study). Main outcomes and measures: We conducted random-effects meta-analysis on our primary outcome, the age-adjusted PS-TL correlation. We investigated potential confounders and moderators (sex, life stress exposure, and PS measure validation) via post hoc subset analyses and meta-regression. Results: Increased PS was associated with a very small decrease in TL (n = 8724 total; r = -0.06; 95% CI: -0.10, -0.008; p = 0.01; alpha = 0.025), adjusting for age. This relationship was similar between sexes and within studies using validated measures of PS, and marginally (nonsignificantly) stronger among samples recruited for stress exposure (r = -0.13; vs. general samples: b = -0.11; 95% CI: -027, 0.01; p = 0.05; alpha = 0.013). Publication bias may exist; correcting for its effects attenuated the relationship. Conclusions and relevance: Our analysis finds a very small, statistically significant relationship between increased PS (as measured over the past month) and decreased TL that may reflect publication bias, although fully parsing the effects of publication bias from other sample-size correlates is challenging, as discussed. The association may be stronger with known major stressors and is similar in magnitude to that noted between obesity and TL. All included studies used single measures of short-term stress; the literature suggests long-term chronic stress may have a larger cumulative effect. Future research should assess for potential confounders and use longitudinal, multidimensional models of stress. (C) 2016 The Authors. Published by Elsevier Inc. C1 [Mathur, Maya B.; Desai, Manisha] Stanford Univ, Quantitat Sci Unit, 1070 Arastradero Rd, Palo Alto, CA 94305 USA. [Epel, Elissa] Univ Calif San Francisco, Dept Psychiat, San Francisco, CA USA. [Kind, Shelley] Boston Univ, Dept Psychol & Brain Sci, Boston, MA 02215 USA. [Parks, Christine G.; Sandler, Dale P.] Natl Inst Environm Hlth Sci, Epidemiol Branch, NIH, Res Triangle Pk, NC USA. [Khazeni, Nayer] Stanford Univ, Div Pulm & Crit Care Med, Stanford, CA 94305 USA. [Khazeni, Nayer] Stanford Univ, Ctr Hlth Policy, Stanford, CA 94305 USA. [Khazeni, Nayer] Stanford Univ, Ctr Primary Care & Outcomes Res, Stanford, CA 94305 USA. RP Mathur, MB (reprint author), Stanford Univ, Quantitat Sci Unit, 1070 Arastradero Rd, Palo Alto, CA 94305 USA. EM mmathur@stanford.edu OI Parks, Christine/0000-0002-5734-3456; Sandler, Dale/0000-0002-6776-0018 FU Agency for Healthcare Research and Policy [1 K08 HS019816]; Wisconsin Partnership Program PERC Award [233 PRJ 25DJ]; National Institutes of Health's Clinical and Translational Science Award [5UL1RR025011]; National Heart, Lung, and Blood Institute [1 RC2 HL101468]; Intramural Research Program of the NIH, National Institute of Environmental Health Sciences [ZO1 ES044005]; Department of Defense Breast Cancer Research Concept Award [BC045286] FX This research was supported by the Agency for Healthcare Research and Policy (1 K08 HS019816, Dr. Khazeni). The Survey of the Health of Wisconsin, from which we obtained raw data, was funded by the Wisconsin Partnership Program PERC Award (233 PRJ 25DJ), the National Institutes of Health's Clinical and Translational Science Award (5UL1RR025011), and the National Heart, Lung, and Blood Institute (1 RC2 HL101468). The Sister Study was supported by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences (ZO1 ES044005), and telomere assays supported in part through by Department of Defense Breast Cancer Research Concept Award (BC045286, Dr. Parks). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 92 TC 7 Z9 9 U1 3 U2 17 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1591 EI 1090-2139 J9 BRAIN BEHAV IMMUN JI Brain Behav. Immun. PD MAY PY 2016 VL 54 BP 158 EP 169 DI 10.1016/j.bbi.2016.02.002 PG 12 WC Immunology; Neurosciences SC Immunology; Neurosciences & Neurology GA DJ4VW UT WOS:000374207200016 PM 26853993 ER PT J AU Vesper, S Wymer, L AF Vesper, Stephen Wymer, Larry TI The relationship between environmental relative moldiness index values and asthma SO INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH LA English DT Review DE Mold; ERMI; Asthma; Area under the curve; Logistic regression ID ENERGY-EFFICIENT HOMES; RISK-FACTORS; EXPOSURE; CHILDREN; ADULTS; MOLDS AB Indoor mold exposures have been qualitatively linked to asthma for more than 25 years. Our goal has been to turn this qualitative link into a quantitative assessment of asthma risk from mold exposures as estimated by the home's environmental relative moldiness index (ERMI) value. The home's ERMI value is derived from the quantitative PCR analysis of 36 molds in a dust sample. Six epidemiological studies of the relationship between ERMI values and asthma, in cities across the U.S., showed that both children and adults with asthma were living in homes with significantly higher ERMI values than the control or comparison homes. Based on these six studies, the accuracy of the ERMI value's link to occupant asthma was analyzed using receiver operating characteristic (ROC) curve and area under the curve (AUC) statistical analysis. The AUC was 0.69 which places the test accuracy in the "fair to good" range for a medical diagnostic test. A logistic regression analysis of the six studies was performed to generate an equation that can be used to predict occupant asthma at specific ERMI values. The ERMI metric may be a useful tool to link the quantification of mold contamination in U.S. homes to some asthma health effects. Published by Elsevier GmbH. C1 [Vesper, Stephen; Wymer, Larry] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. RP Vesper, S (reprint author), US EPA, 26 West ML King Dr, Cincinnati, OH 45268 USA. EM vesper.stephen@epa.gov FU U.S. Environmental Protection Agency (EPA) through its Office of Research and Development FX The U.S. Environmental Protection Agency (EPA) through its Office of Research and Development funded in the research described here. It has been subjected to the Agency's peer review and has been approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by the EPA for use. Since MSQPCR technology is patented by the US EPA, the Agency has a financial interest in its commercial use. NR 33 TC 3 Z9 3 U1 4 U2 8 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1438-4639 EI 1618-131X J9 INT J HYG ENVIR HEAL JI Int. J. Hyg. Environ. Health. PD MAY PY 2016 VL 219 IS 3 BP 233 EP 238 DI 10.1016/j.ijheh.2016.01.006 PG 6 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA DJ4SR UT WOS:000374198800001 PM 26861576 ER PT J AU McMinn, BR Korajkic, A Grimm, AC AF McMinn, Brian R. Korajkic, Asja Grimm, Ann C. TI Optimization and evaluation of a method to detect adenoviruses in river water SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Adenovirus; River water; Small volume; Improved detection ID NATURAL ORGANIC-MATTER; TIME PCR ASSAY; ENTERIC VIRUSES; ACUTE GASTROENTERITIS; MICROPOROUS FILTER; LARGE VOLUMES; WASTE-WATER; TAP WATER; SAMPLES; QUANTIFICATION AB Adenoviruses are often implicated in recreational water disease outbreaks but existing methods for their detection perform poorly within these matrices. In this study, small volume (100 mL) concentration was used to identify processes that promoted recovery of adenovirus from river water. Several alternative secondary concentration techniques were investigated and compared to the baseline method consisting of primary concentration via filtration, followed by celite mediated secondary concentration. The alternative secondary concentrations included multiple filter elutions, soaking the filter for 15 min prior to elution and concentration using pre-treated celite (river water, 1.5% and 3% milk) instead of a filter. Modifications of the viral nucleic acid extraction technique were also evaluated. Concentration using pre-treated celite and a modified extraction technique (10 min boil and a 1 h ProK incubation at 37 degrees C) recovered significantly higher levels of adenovirus (P=0.001) than other methods tested. This optimized method increased recovery of spiked adenovirus (57 +/- 27%) compared to baseline method performance (4 3%) indicating that use of pre-treated celite as opposed to filtration significantly improves recovery. Application of the optimized concentration method to larger volume (1 L) of river water resulted in similar recoveries (42 +/- 19%) underlying the utility of this method to detect adenovirus from environmental samples. (C) 2016 Published by Elsevier B.V. C1 [McMinn, Brian R.; Korajkic, Asja; Grimm, Ann C.] US EPA, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. RP McMinn, BR (reprint author), US EPA, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. EM Mcminn.brian@epa.gov FU United States Environmental Protection Agency through its Office of Research and Development FX The United States Environmental Protection Agency through its Office of Research and Development funded and managed the research described here. It has been subjected to Agency's administrative review and approved for publication. The views expressed in this article are those of the author(s) and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 45 TC 0 Z9 0 U1 3 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD MAY PY 2016 VL 231 BP 8 EP 13 DI 10.1016/j.jviromet.2016.02.003 PG 6 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA DJ4TH UT WOS:000374200400002 PM 26874286 ER PT J AU Lee, EH Beedlow, PA Waschmann, RS Tingey, DT Wickham, C Cline, S Bollman, M Carlile, C AF Lee, E. Henry Beedlow, Peter A. Waschmann, Ronald S. Tingey, David T. Wickham, Charlotte Cline, Steve Bollman, Michael Carlile, Cailie TI Douglas-fir displays a range of growth responses to temperature, water, and Swiss needle cast in western Oregon, USA SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Climate change; Dendrochronology; Douglas-fir; Pacific Decadal Oscillation; Pacific Northwest; Swiss needle cast ID CARBON-ISOTOPE DISCRIMINATION; BRITISH-COLUMBIA; PACIFIC-NORTHWEST; CLIMATE-CHANGE; RADIAL GROWTH; PSEUDOTSUGA-MENZIESII; GEOGRAPHIC-VARIATION; CO2 ASSIMILATION; SPRUCE BUDWORM; TREE MORTALITY AB Douglas-fir (Pseudotsuga menziesii var. menziesii (Mirb.) Franco) growth in the Pacific Northwest is affected,by climatic, edaphic factors and Swiss needle cast (SNC) disease. We examine Douglas-fir growth responses to temperature, dewpoint deficit (DPD), soil moisture, and SNC using time series intervention analysis of intra-annual tree-ring width data collected at nine forest stands in western Oregon, USA. Air temperature, previous-year DPD and SNC and their interactions were the primary factors influencing tree growth at all sites, whereas other key seasonal climatic factors limiting growth varied by site. Winter temperature was more important at high elevation cool sites, whereas summer temperature was more important at warm and dry sites. Growth rate increased with summer temperature to an optimum (T-opt) then decreased at higher temperatures. At drier sites, temperature and water affected growth interactively such that T-opt decreased with decreasing summer soil moisture. With increasing temperature due to climate change, growth rates increased at high elevation sites and declined at mid-elevation inland sites since 1990. Growth response to climate and SNC are confounded at all sites. We conclude that as temperature rises and precipitation patterns shift toward wetter winters and drier summers, Douglas-fir will experience greater temperature and water stress and an increase in severity of SNC. Published by Elsevier B.V. C1 [Lee, E. Henry; Beedlow, Peter A.; Waschmann, Ronald S.; Tingey, David T.; Cline, Steve; Bollman, Michael] US EPA, 200 SW 35th St, Corvallis, OR 97333 USA. [Wickham, Charlotte] Oregon State Univ, Dept Stat, Corvallis, OR 97332 USA. [Carlile, Cailie] Missouri Dept Nat Resources, Jefferson City, MO 65102 USA. RP Lee, EH (reprint author), US EPA, 200 SW 35th St, Corvallis, OR 97333 USA. EM lee.ehenry@epa.gov FU U.S. Environmental Protection Agency FX The authors thank Dr. Rene Alfaro for his thoughtful review and helpful suggestions and Dr. Donald Phillips, Katie Steele and Erin Corrigan for their valuable assistance in the collection and processing of tree core samples. The authors thank Jonathan Halama for GIS assistance in mapping the study sites. The research described in this article has been funded wholly by the U.S. Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory's Western Ecology Division and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 72 TC 0 Z9 0 U1 13 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAY 1 PY 2016 VL 221 BP 176 EP 188 DI 10.1016/j.agrformet.2016.02.009 PG 13 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DJ2ZM UT WOS:000374074200015 ER PT J AU Breen, M Villeneuve, DL Ankley, GT Bencic, D Breen, MS Watanabe, KH Lloyd, AL Conolly, RB AF Breen, Miyuki Villeneuve, Daniel L. Ankley, Gerald T. Bencic, David Breen, Michael S. Watanabe, Karen H. Lloyd, Alun L. Conolly, Rory B. TI Computational model of the fathead minnow hypothalamic-pituitary-gonadal axis: Incorporating protein synthesis in improving predictability of responses to endocrine active chemicals SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY LA English DT Article DE Adaptation; Computational model; Endocrine disrupting chemicals; Fadrozole; Fish; Hypothalamic-pituitary-gonadal axis; Protein synthesis; Toxicology ID CHARACTERIZE ADAPTIVE RESPONSES; IN-VITRO; INHIBITION; FISH; BIOMARKERS; DISRUPTORS AB There is international concern about chemicals that alter endocrine system function in humans and/or wildlife and subsequently cause adverse effects. We previously developed a mechanistic computational model of the hypothalamic-pituitary-gonadal (HPG) axis in female fathead minnows exposed to a model aromatase inhibitor, fadrozole (FAD), to predict dose-response and time-course behaviors for apical reproductive endpoints. Initial efforts to develop a computational model describing adaptive responses to endocrine stress providing good fits to empirical plasma 17 beta-estradiol (E2) data in exposed fish were only partially successful, which suggests that additional regulatory biology processes need to be considered. In this study, we addressed short-comings of the previous model by incorporating additional details concerning CYP19A (aromatase) protein synthesis. Predictions based on the revised model were evaluated using plasma E2 concentrations and ovarian cytochrome P450 (CYP) 19 A aromatase mRNA data from two fathead minnow time-course experiments with FAD, as well as from a third 4-day study. The extended model provides better fits to measured E2 time-course concentrations, and the model accurately predicts CYP19A mRNA fold changes and plasma E2 dose-response from the 4-d concentration-response study. This study suggests that aromatase protein synthesis is an important process in the biological system to model the effects of FAD exposure. (C) 2016 Published by Elsevier Inc. C1 [Breen, Miyuki; Lloyd, Alun L.] N Carolina State Univ, Dept Math, Biomath Grad Program, Box 8203, Raleigh, NC 27695 USA. [Villeneuve, Daniel L.; Ankley, Gerald T.] US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Bencic, David] US EPA, Ecol Exposure Res Div, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [Breen, Michael S.] US EPA, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Watanabe, Karen H.] Oregon Hlth & Sci Univ, Inst Environm Hlth, Div Environm & Biomol Syst, 3181 SW Sam Jackson Pk Rd HRC3, Portland, OR 97239 USA. [Conolly, Rory B.] US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. RP Conolly, RB (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr,Mail B105-03, Res Triangle Pk, NC 27711 USA. EM mbreen@ncsu.edu; villeneuve.dan@epa.gov; ankley.gerald@epa.gov; bencic.david@epa.gov; breen.michael@epa.gov; watanabk@ohsu.edu; alun_lloyd@ncsu.edu; conolly.rory@epa.gov NR 23 TC 2 Z9 2 U1 5 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1532-0456 EI 1878-1659 J9 COMP BIOCHEM PHYS C JI Comp. Biochem. Physiol. C-Toxicol. Pharmacol. PD MAY-JUN PY 2016 VL 183 BP 36 EP 45 DI 10.1016/j.cbpc.2016.02.002 PG 10 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology GA DI3QO UT WOS:000373413800005 PM 26875912 ER PT J AU Harding, LW Gallegos, CL Perry, ES Miller, WD Adolf, JE Mallonee, ME Paerl, HW AF Harding, L. W., Jr. Gallegos, C. L. Perry, E. S. Miller, W. D. Adolf, J. E. Mallonee, M. E. Paerl, H. W. TI Long-Term Trends of Nutrients and Phytoplankton in Chesapeake Bay SO ESTUARIES AND COASTS LA English DT Article DE Estuaries; Chesapeake Bay; Long-term trends; Hydrology; Eutrophication; Water quality; Phytoplankton; Nutrients; Chlorophyll ID CONTIGUOUS UNITED-STATES; NORTH-CAROLINA; COASTAL EUTROPHICATION; SYNOPTIC CLIMATOLOGY; ESTUARINE ECOSYSTEM; PAMLICO SOUND; WATER-QUALITY; RIVER ESTUARY; BIOMASS; CHLOROPHYLL AB Climate effects on hydrology impart high variability to water-quality properties, including nutrient loadings, concentrations, and phytoplankton biomass as chlorophyll-a (chl-a), in estuarine and coastal ecosystems. Resolving long-term trends of these properties requires that we distinguish climate effects from secular changes reflecting anthropogenic eutrophication. Here, we test the hypothesis that strong climatic contrasts leading to irregular dry and wet periods contribute significantly to interannual variability of mean annual values of water-quality properties using in situ data for Chesapeake Bay. Climate effects are quantified using annual freshwater discharge from the Susquehanna River together with a synoptic climatology for the Chesapeake Bay region based on predominant sea-level pressure patterns. Time series of water-quality properties are analyzed using historical (1945-1983) and recent (1984-2012) data for the bay adjusted for climate effects on hydrology. Contemporary monitoring by the Chesapeake Bay Program (CBP) provides data for a period since mid-1984 that is significantly impacted by anthropogenic eutrophication, while historical data back to 1945 serve as historical context for a period prior to severe impairments. The generalized additive model (GAM) and the generalized additive mixed model (GAMM) are developed for nutrient loadings and concentrations (total nitrogen-TN, nitrate + nitrate-NO2 + NO3) at the Susquehanna River and water-quality properties in the bay proper, including dissolved nutrients (NO2 + NO3, orthophosphate-PO4), chl-a, diffuse light attenuation coefficient (K (D) (PAR)), and chl-a/TN. Each statistical model consists of a sum of nonlinear functions to generate flow-adjusted time series and compute long-term trends accounting for climate effects on hydrology. We present results identifying successive periods of (1) eutrophication ca. 1945-1980 characterized by approximately doubled TN and NO2 + NO3 loadings, leading to increased chl-a and associated ecosystem impairments, and (2) modest decreases of TN and NO2 + NO3 loadings from 1981 to 2012, signaling a partial reversal of nutrient over-enrichment. Comparison of our findings with long-term trends of water-quality properties for a variety of estuarine and coastal ecosystems around the world reveals that trends for Chesapeake Bay are weaker than for other systems subject to strenuous management efforts, suggesting that more aggressive actions than those undertaken to date will be required to counter anthropogenic eutrophication of this valuable resource. C1 [Harding, L. W., Jr.] Univ Calif Los Angeles, Dept Atmospher & Ocean Sci, Los Angeles, CA 90095 USA. [Gallegos, C. L.] Smithsonian Environm Res Ctr, POB 28, Edgewater, MD 21037 USA. [Perry, E. S.] 2000 Kings Landing Rd, Huntingtown, MD 20639 USA. [Miller, W. D.] US Navy, Res Lab, 4555 Overlook Ave SW, Washington, DC 20375 USA. [Adolf, J. E.] Univ Hawaii, Marine Sci Program, 200 W Kawili St, Hilo, HI 96720 USA. [Mallonee, M. E.] US EPA, Chesapeake Bay Program Off, Interstate Commiss Potomac River Basin, 410 Severn Ave, Annapolis, MD 21403 USA. [Paerl, H. W.] Univ North Carolina Chapel Hill, Inst Marine Sci, 3431 Arendell St, Morehead City, NC 28557 USA. RP Harding, LW (reprint author), Univ Calif Los Angeles, Dept Atmospher & Ocean Sci, Los Angeles, CA 90095 USA. EM lharding@atmos.ucla.edu OI Gallegos, Charles/0000-0001-5112-0166; Miller, W. David/0000-0002-4940-5987 FU NSF Biological Oceanography Program; NOAA Chesapeake Bay Program Office; North Carolina Department of Environment and Natural Resources; ModMon and FerryMon Projects; Strategic Environmental Defense and Development Program (SERDP) of the Department of Defense FX Our thanks to Dr. Jim Hagy of the EPA Gulf Breeze Laboratory for providing data from his Ph.D. Dissertation and to Dr. Bob Hirsch and his colleagues at the US Geological Survey for providing data on nutrient concentrations and loadings from the Nontidal Monitoring Program. Rich Batiuk and Gary Shenk of the EPA Chesapeake Bay Program provided point-source nutrient estimates and helpful comments on the manuscript. Detailed comments and suggestions by two anonymous reviewers significantly improved the manuscript. LWH was supported by the NSF Biological Oceanography Program and the NOAA Chesapeake Bay Program Office. HWP was supported by the NSF Biological Oceanography Program, the North Carolina Department of Environment and Natural Resources, ModMon and FerryMon Projects, and the Strategic Environmental Defense and Development Program (SERDP) of the Department of Defense. NR 72 TC 4 Z9 4 U1 19 U2 69 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1559-2723 EI 1559-2731 J9 ESTUAR COAST JI Estuaries Coasts PD MAY PY 2016 VL 39 IS 3 BP 664 EP 681 DI 10.1007/s12237-015-0023-7 PG 18 WC Environmental Sciences; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DI2WS UT WOS:000373360500006 ER PT J AU Dong, Z Lewis, CG Burgess, RM Coull, B Shine, JP AF Dong, Zhao Lewis, Christopher G. Burgess, Robert M. Coull, Brent Shine, James P. TI Statistical evaluation of biogeochemical variables affecting spatiotemporal distributions of multiple free metal ion concentrations in an urban estuary SO CHEMOSPHERE LA English DT Article DE Metal mixtures; Free metal ions; Biogeochemical factors; Passive sampler; Spatiotemporal variability; Urban estuary ID DISSOLVED ORGANIC-MATTER; BIOTIC LIGAND MODEL; EQUILIBRIUM-BASED SAMPLER; COASTAL SEDIMENTS; MARINE-SEDIMENTS; WATER-QUALITY; BOSTON HARBOR; TOXICITY; SPECIATION; COPPER AB Free metal ion concentrations have been recognized as a better indicator of metal bioavailability in aquatic environments than total dissolved metal concentrations. However, our understanding of the determinants of free ion concentrations, especially in a metal mixture, is limited, due to underexplored techniques for measuring multiple free metal ions simultaneously. In this work, we performed statistical analyses on a large dataset containing repeated measurements of free ion concentrations of Cu, Zn, Pb, Ni, and Cd, the most commonly measured metals in seawater, at five inshore locations in Boston Harbor, previously collected using an in-situ equilibrium-based multi-metal free ion sampler, the 'Gellyfish'. We examined correlations among these five metals by season, and evaluated effects of 10 biogeochemical variables on free ion concentrations over time and location through multivariate regressions. We also explored potential clustering among the five metals through a principal component analysis. We found significant correlations among metals, with varying patterns over season. Our regression results suggest that instead of dissolved metals, pH, salinity, temperature and rainfall were the most significant determinants of free metal ion concentrations. For example, a one-unit decrease in pH was associated with a 2.2 (Cd) to 99 (Cu) times increase in free ion concentrations. This work is among the first to reveal key contributors to spatiotemporal variations in free ion concentrations, and demonstrated the usefulness of the Gellyfish sampler in routine sampling of free ions within metal mixtures and in generating data for statistical analyses. (C) 2016 Published by Elsevier Ltd. C1 [Dong, Zhao; Lewis, Christopher G.; Shine, James P.] Harvard Univ, TH Chan Sch Publ Hlth, Dept Environm Hlth, 665 Huntington Ave, Boston, MA 02115 USA. [Burgess, Robert M.] US EPA, ORD NHEERL Atlantic Ecol Div, Narragansett, RI USA. [Coull, Brent] Harvard Univ, TH Chan Sch Publ Hlth, Dept Biostat, 665 Huntington Ave, Boston, MA 02115 USA. RP Dong, Z (reprint author), 401 Pk Dr,Landmark Ctr West 415, Boston, MA 02215 USA. EM zdong@hsph.harvard.edu RI Dong, Zhao/Q-2518-2015 OI Dong, Zhao/0000-0002-6188-1337 FU National Institute of Environmental Health Sciences (NIEHS) [P42 ES05947] FX This work was funded by Grant P42 ES05947 from the National Institute of Environmental Health Sciences (NIEHS). The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of the NIEHS, NIH. We thank Dr. Laurel Schaider for proofreading this manuscript. NR 50 TC 0 Z9 0 U1 2 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD MAY PY 2016 VL 150 BP 202 EP 210 DI 10.1016/j.chemosphere.2016.02.020 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DH4OK UT WOS:000372765100026 PM 26901477 ER PT J AU Vanderhoof, MK Alexander, LC Todd, MJ AF Vanderhoof, Melanie K. Alexander, Laurie C. Todd, M. Jason TI Temporal and spatial patterns of wetland extent influence variability of surface water connectivity in the Prairie Pothole Region, United States SO LANDSCAPE ECOLOGY LA English DT Article DE Wetland inundation; Wetland-stream connectivity; Geographically isolated wetlands; Prairie Pothole Region; Landsat; Interannual variability ID DROUGHT SEVERITY INDEX; GREAT-PLAINS; NORTH-DAKOTA; SOUTH-DAKOTA; LANDSCAPES; QUALITY; TRANSFORMATION; DISTRIBUTIONS; CALIFORNIA; DATASET AB Quantifying variability in landscape-scale surface water connectivity can help improve our understanding of the multiple effects of wetlands on downstream waterways. We examined how wetland merging and the coalescence of wetlands with streams varied both spatially (among ecoregions) and interannually (from drought to deluge) across parts of the Prairie Pothole Region. Wetland extent was derived over a time series (1990-2011) using Landsat imagery. Changes in landscape-scale connectivity, generated by the physical coalescence of wetlands with other surface water features, were quantified by fusing static wetland and stream datasets with Landsat-derived wetland extent maps, and related to multiple wetness indices. The usage of Landsat allows for decadal-scale analysis, but limits the types of surface water connections that can be detected. Wetland extent correlated positively with the merging of wetlands and wetlands with streams. Wetness conditions, as defined by drought indices and runoff, were positively correlated with wetland extent, but less consistently correlated with measures of surface water connectivity. The degree of wetland-wetland merging was found to depend less on total wetland area or density, and more on climate conditions, as well as the threshold for how wetland/upland was defined. In contrast, the merging of wetlands with streams was positively correlated with stream density, and inversely related to wetland density. Characterizing the degree of surface water connectivity within the Prairie Pothole Region in North America requires consideration of (1) climate-driven variation in wetness conditions and (2) within-region variation in wetland and stream spatial arrangements. C1 [Vanderhoof, Melanie K.] US Geol Survey, Geosci & Environm Change Sci Ctr, DFC, POB 25046,MS980, Lakewood, CO 80225 USA. [Alexander, Laurie C.; Todd, M. Jason] US EPA, Off Res & Dev, Natl Ctr Environm Assessment, 1200 Penn Ave NW, Washington, DC 20460 USA. RP Vanderhoof, MK (reprint author), US Geol Survey, Geosci & Environm Change Sci Ctr, DFC, POB 25046,MS980, Lakewood, CO 80225 USA. EM melvanderhoof@gmail.com FU U.S. EPA Office of Research and Development, National Center for Environmental Assessment FX This project was supported in part by an appointment to the Internship/Research Participation Program at the U.S. Environmental Protection Agency, Office of Research and Development, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. This work was also funded by the U.S. EPA Office of Research and Development, National Center for Environmental Assessment. We thank Megan Lang and Greg McCarty at USDA for their logistical support, and Scott Leibowitz, Megan Lang, Jay Christensen, Heather Golden and Scot Hagerthey for their comments on earlier versions of the manuscript. We also thank the anonymous reviewers for their comments to improve the manuscript. The views expressed in this manuscript are solely those of the authors and do not necessarily reflect the views or policies of the U.S. EPA. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 61 TC 6 Z9 7 U1 3 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD MAY PY 2016 VL 31 IS 4 BP 805 EP 824 DI 10.1007/s10980-015-0290-5 PG 20 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DG8FZ UT WOS:000372319700009 ER PT J AU Li, WW Morgan, MK Graham, SE Starr, JM AF Li, Weiwei Morgan, Marsha K. Graham, Stephen E. Starr, James M. TI Measurement of pyrethroids and their environmental degradation products in fresh fruits and vegetables using a modification of the quick easy cheap effective rugged safe (QuEChERS) method SO TALANTA LA English DT Article DE QuEChERS method; Pyrethroids; Environmental degradates; Solid phase extraction; MDLs/MQLs; LC-MS/MS ID DAY-CARE-CENTERS; PESTICIDE-RESIDUES; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; HOMES; METABOLITES; VALIDATION; EXPOSURE; SAMPLES AB Pyrethroid insecticides are used extensively in agriculture, and they, as well as their environmental degradates, may remain as residues on foods such as fruits and vegetables. Since pyrethroid degradates can be identical to the urinary markers used in human biomonitoring, it is important to understand the contribution of these degradates when studying sources of human pyrethroid exposure. We modified the widely used Quick Easy Cheap Effective Rugged Safe (QuEChERS) method to measure several current-use pyrethroids (cis/trans-permethrin, cypermethrin, deltamethrin, esfenvalerate, bifenthrin, cyfluthrin, and cyhalothrin) and their environmental degradation products (3-PBA, cis/trans-DCCA, 4-F-3-PBA, DBCA, and MPA) in selected fresh fruits and vegetables. Using fortified samples, we determined extraction efficiencies from: tomatoes, oranges (whole, peeled, and rind), grapes, apples, bananas (peeled and rind only), onions, lettuce, green peppers, carrots and broccoli. For a subset of these food items (apples, grapes, tomatoes, lettuce and banana peel), we also established limits of detection (MDLs) and quantitation (MQLs). Each sample was homogenized (1 kg) then spiked with the target pyrethroids and their degradation products. Sub-samples (15 g) were extracted with acetonitrile, then salted out and partitioned with NaCl and MgSO4. The extract was divided and further cleaned using solid phase extraction (SPE) cartridges containing either graphitized non-porous carbon (pyrethroids) or C-18 (degradation products). Sample analysis was via liquid chromatography/tandem mass spectrometry (LC-MS/MS). Considering the mean recoveries each of the 14 analytes in all 13 matrices: 42% of the recoveries were >= 90%, 70% were >= 80%, and 90% were >= 70%. All MDL's were less than 100 ng/kg, except 3-PBA (132 ng/kg, tomato), MPA (129 ng/kg, tomato), and trans-permethrin (141 ng/kg, banana peel). We then applied the method to non-spiked samples (subset of 5 for which the MDLs/MQLs had been determined) collected weekly for four weeks from local supermarkets. At least one pyrethroid was present in measureable concentrations in all matrices except banana peels. In contrast, the only degradation products detected were cis/trans-DCCA, in one lettuce sample. Published by Elsevier B.V. C1 [Li, Weiwei; Morgan, Marsha K.; Starr, James M.] US EPA, Off Res & Dev, Natl Exposure Res Lab, USEPA MD D205-05,109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Graham, Stephen E.] US EPA, Off Air Qual Planning & Stand, Hlth & Environm Impacts Div, Res Triangle Pk, NC 27711 USA. RP Starr, JM (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab, USEPA MD D205-05,109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM starr.james@epa.gov FU United States Environmental Protection Agency through its Office of Research and Development FX This work was supported by The United States Environmental Protection Agency through its Office of Research and Development who funded and managed the research described here. It has been subjected to Agency administrative review and approved for publication. This does not signify that the contents necessarily reflect the views and policies of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 24 TC 3 Z9 4 U1 18 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0039-9140 EI 1873-3573 J9 TALANTA JI Talanta PD MAY 1 PY 2016 VL 151 BP 42 EP 50 DI 10.1016/j.talanta.2016.01.009 PG 9 WC Chemistry, Analytical SC Chemistry GA DH4NE UT WOS:000372761900006 PM 26946008 ER PT J AU Macedo, DR Hughes, RM Ferreira, WR Firmiano, KR Silva, DRO Ligeiro, R Kaufmann, PR Callisto, M AF Macedo, Diego R. Hughes, Robert M. Ferreira, Wander R. Firmiano, Kele R. Silva, Deborah R. O. Ligeiro, Raphael Kaufmann, Philip R. Callisto, Marcos TI Development of a benthic macroinvertebrate multimetric index (MMI) for Neotropical Savanna headwater streams SO ECOLOGICAL INDICATORS LA English DT Article DE Anthropogenic pressures; Bioassessment; Land use and cover assessment; Site habitat assessment; Benthic macroinvertebrates ID BIOTIC INTEGRITY; LAND-USE; ECOLOGICAL CONDITION; BIOLOGICAL CONDITION; AQUATIC ECOSYSTEMS; NATIONAL ASSESSMENT; BRAZILIAN CERRADO; WADEABLE STREAMS; BED STABILITY; WESTERN USA AB Assessing the ecological impacts of anthropogenic pressures is a key task in environmental management. Multimetric indices (MMIs), based on aquatic assemblage responses to anthropogenic pressures, have been used increasingly throughout the world. The MMI approach is a low-cost, rapid field method that produces an aquatic condition index that responds precisely to anthropogenic pressures, making it useful for conservation and environmental management. We developed four candidate MMIs based on benthic macroinvertebrate assemblages sampled at 40 randomly selected sites to assess the environmental condition of streams upstream of a hydroelectric power plant in the Brazilian Neotropical Savanna biome. Those MMIs were built from landscape-adjusted and unadjusted biological metrics as well as two alternative ways of choosing metrics. The alternative MMIs performances were tested by comparing their precision to distinguish least-disturbed areas, responsiveness to discriminate least-and most-disturbed areas, and sensitivity to anthropogenic pressures at catchment and local scales. The best performing MMI had landscape-adjusted metrics and was produced through use of principal component analysis for metric selection. It included 4 metrics: Ephemeroptera richness, average tolerance score per taxon, percentage of predator individuals, and percentage of Odonata individuals adjusted by elevation. This index discriminated well the anthropogenic pressures at local-and catchment-scales, and at both scales simultaneously, as indicated by an integrated disturbance index. Our methodological development included statistical criteria for identifying least-and most-disturbed sites, calibrating for natural landscape variability, and use of non-redundant metrics. Therefore, we expect it will provide a model for environmental assessment of water resources elsewhere in Brazil and in other nations. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Macedo, Diego R.; Ferreira, Wander R.; Firmiano, Kele R.; Silva, Deborah R. O.; Callisto, Marcos] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, Lab Ecol Bentos, Ave Antonio Carlos 6627,CP 486, BR-31270901 Belo Horizonte, MG, Brazil. [Hughes, Robert M.] Oregon State Univ, Amnis Opes Inst, 104 Nash Hall, Corvallis, OR 97331 USA. [Hughes, Robert M.] Oregon State Univ, Dept Fisheries & Wildlife, 104 Nash Hall, Corvallis, OR 97331 USA. [Ligeiro, Raphael] Fed Univ Para, Inst Ciencias Biol, Rua Augusto Correa 01, BR-66075110 Belem, Para, Brazil. [Kaufmann, Philip R.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Lab, Western Ecol Div, 200 SW 35 St, Corvallis, OR 97333 USA. [Macedo, Diego R.] Univ Fed Minas Gerais, Inst Geociencias, Dept Geog, Ave Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG, Brazil. RP Macedo, DR (reprint author), Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, Lab Ecol Bentos, Ave Antonio Carlos 6627,CP 486, BR-31270901 Belo Horizonte, MG, Brazil.; Macedo, DR (reprint author), Univ Fed Minas Gerais, Inst Geociencias, Dept Geog, Ave Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG, Brazil. EM rodriguesmacedo@gmail.com RI Macedo, Diego/G-2506-2015 OI Macedo, Diego/0000-0002-1178-4969 FU Companhia Energetica de Minas Gerais (CEMIG-Programa Peixe Vivo); Pesquisa e Desenvolvimento da Agencia Nacional de Energia Eletrica [GT-487]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [302960/2011-2]; Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG); Fulbright Brasil. Colleagues from the Centro Federal de Educacao Tecnologica de Minas Gerais; Pontificia Unversidade Catolica de Minas Gerais; Universidade Federal de Lavras; Universidade Federal de Minas Gerais assisted; FAPEMIG [PPM-77/13, PPM IX-02/2015] FX We received funding from Companhia Energetica de Minas Gerais (CEMIG-Programa Peixe Vivo), Pesquisa e Desenvolvimento da Agencia Nacional de Energia Eletrica (P&D ANEEL-CEMIG GT-487), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), and Fulbright Brasil. Colleagues from the Centro Federal de Educacao Tecnologica de Minas Gerais, Pontificia Unversidade Catolica de Minas Gerais, Universidade Federal de Lavras, and Universidade Federal de Minas Gerais assisted with field collections and laboratory work. Juliana Franca and Ana Paula Eller were responsible for water quality analyses. Carlos B.M. Alves helped with general logistics and fieldwork. MC was awarded research productivity grants CNPq (No. 302960/2011-2) and Minas Gerais researcher grant FAPEMIG PPM-77/13 and PPM IX-02/2015. We are grateful for assistance in statistical design and site selection from Tony Olsen, Marc Weber, and Phil Larsen and manuscript review by Andy Rehn and two anonymous journal reviewers. This manuscript was subjected to review by the U.S. Environmental Protection Agency National Health and Environmental Effects Research Laboratory's Western Ecology Division and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 89 TC 5 Z9 5 U1 10 U2 61 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD MAY PY 2016 VL 64 BP 132 EP 141 DI 10.1016/j.ecolind.2015.12.019 PG 10 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DF1KN UT WOS:000371098700013 ER PT J AU Gabriel, M Knightes, C Cooter, E Dennis, R AF Gabriel, Mark Knightes, Christopher Cooter, Ellen Dennis, Robin TI Evaluating relative sensitivity of SWAT-simulated nitrogen discharge to projected climate and land cover changes for two watersheds in North Carolina, USA SO HYDROLOGICAL PROCESSES LA English DT Article DE GCM; climate change; nitrogen; land cover; CO2; watershed ID CHANGE IMPACTS; RIVER-BASIN; ASSESSMENT-TOOL; NUTRIENT MANAGEMENT; MODELING APPROACH; FUTURE CLIMATE; QUALITY; HYDROLOGY; CATCHMENT; SYSTEM AB We investigated how projected changes in land cover and climate affected simulated nitrate (NO3-) and organic nitrogen discharge for two watersheds within the Neuse River Basin, North Carolina, USA, for years 2010-2070. We applied the Soil and Water Assessment Tool watershed model to predict nitrogen discharge using (1) atmospheric carbon dioxide (CO2) concentrations predicted by the Intergovernmental Panel on Climate Change, (2) land cover change predicted by the Integrated Climate and Land Use Change project and (3) precipitation and temperature simulated by two statistically downscaled and bias-corrected Global Circulation Models. We determined the sensitivity of simulated nitrogen discharge to separate changes in each treatment [(1) CO2, (2) land cover and (3) precipitation and temperature (PT)] by comparing each treatment to a reference condition. Results showed that nitrogen discharges were most sensitive to changes in PT over the 60-year simulation. Nitrogen discharges had similar sensitivities to the CO2 and land cover treatments, which were only one-tenth the influence of the PT treatment. Under the CO2 treatment, nitrogen discharges increased with increasing ambient CO2. NO3- discharge decreased with increased urbanization; however, organic nitrogen had a varied response. Under the PT treatment, there was high spatial variability in nitrogen discharges. In a single year, certain sub-basins showed an 80% increase in nitrogen discharge relative to reference, while others showed a 400% decrease. With nitrogen discharge showing high sensitivity to PT change, we suggest that more emphasis should be placed on investigating impacts of PT on nutrient transport in the Neuse River Basin. Published 2015. This article is a U.S. Government work and is in the public domain in the USA C1 [Gabriel, Mark; Knightes, Christopher] US EPA, ORD, NERL, ERD, 960 Coll Stn Rd, Athens, GA 30605 USA. [Cooter, Ellen; Dennis, Robin] US EPA, ORD, NERL, AMAD, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. RP Gabriel, M (reprint author), Int Joint Commiss, 2000 L St,NW,Suite 615, Washington, DC 20036 USA. EM marklive02@yahoo.com NR 61 TC 1 Z9 1 U1 7 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD APR 30 PY 2016 VL 30 IS 9 BP 1403 EP 1418 DI 10.1002/hyp.10707 PG 16 WC Water Resources SC Water Resources GA DJ7HZ UT WOS:000374383800008 ER PT J AU Strickland, JD LeFew, WR Crooks, J Hall, D Ortenzio, JNR Dreher, K Shafer, TJ AF Strickland, Jenna D. LeFew, William R. Crooks, James Hall, Diana Ortenzio, Jayna N. R. Dreher, Kevin Shafer, Timothy J. TI In vitro screening of silver nanoparticles and ionic silver using neural networks yields differential effects on spontaneous activity and pharmacological responses SO TOXICOLOGY LA English DT Article DE Nanoparticles; Screening; Neurophysiology; Silver; Function; Microelectrode arrays ID SPRAGUE-DAWLEY RATS; MICROELECTRODE ARRAYS; ENGINEERED NANOMATERIALS; PRIMARY CULTURES; ORAL-EXPOSURE; NANO-AG; NEURONS; NEUROTOXICITY; NANOSILVER; DOSIMETRY AB Silver nanoparticles (AgNPs) are used in a wide range of consumer and medical products because of their antimicrobial and antifungal properties, and can translocate to the brain following exposure. Therefore, to screen AgNPs for potential impacts on human health, it is essential to examine neural function. The present study examined AgNPs (3 citrate coated, 3 PVP coated; 10-75 nm) and AgNO3 effects on spontaneous and pharmacologically-induced neural network function in rat primary cortical cells on multi-well microelectrode array (mwMEA) plates. Baseline activity (1 h) was recorded prior to exposure to non-cytotoxic concentrations of AgNPs and AgNO3 (0.08-0.63 and 0.08-1.7 mu g/ml, respectively). Changes in number of total extracellularly-recorded action potential spikes (total spikes; TS) and active electrodes (AE), relative to controls, were assessed 1, 24, and 48 h after exposure to AgNP suspensions or AgNO3. After the 48 h recording, the response to a pharmacological challenge with the GABA(A) antagonist, bicuculline (BIC), was assessed. Only two particles altered neural network function. Citrate coated 10 nm AgNP caused concentration-related increases in AEs at 24 h. After BIC treatment, PVP coated 75 nm AgNP caused concentration-dependent increases in AE. AgNO3 effects differed from AgNPs, causing a concentration-related decrease in AEs at 24 and 48 h, and a concentration-related decrease in TS following BIC challenge. Importantly, the direction of AgNO3 effects on neural activity was opposite those of 10 nm Ag citrate at concentrations up to 0.63 mu g/ml, and different from 75 nm Ag PVP, indicating ionic silver does not mediate these effects. These results demonstrate that non-cytotoxic concentrations of 10 nm citrate- and 75 nm PVP-coated Ag NPs alter neural network function in vitro, and should be considered for additional neurotoxicity hazard characterization. Published by Elsevier Ireland Ltd. C1 [Strickland, Jenna D.; LeFew, William R.; Crooks, James; Hall, Diana; Ortenzio, Jayna N. R.; Dreher, Kevin; Shafer, Timothy J.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Ortenzio, Jayna N. R.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. RP Shafer, TJ (reprint author), US EPA, Integrated Syst Toxicol Div, Off Res & Dev, MD B105-03, Res Triangle Pk, NC 27711 USA. EM Shafer.tim@epa.gov OI Shafer, Timothy/0000-0002-8069-9987 FU U.S. Environmental Protection Agency; [EP-11D-000392]; [EP-13-D-000108] FX Preparation of this document has been funded by the U.S. Environmental Protection Agency. This document has been reviewed by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.; These authors were supported by student services contracts #EP-11D-000392 and #EP-13-D-000108, respectively. NR 45 TC 1 Z9 1 U1 7 U2 15 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PD APR 29 PY 2016 VL 355 BP 1 EP 8 DI 10.1016/j.tox.2016.05.009 PG 8 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA DP4EA UT WOS:000378447800001 PM 27179409 ER PT J AU Edmonds, J Lindquist, HDA Sabol, J Martinez, K Shadomy, S Cymet, T Emanuel, P AF Edmonds, Jason Lindquist, H. D. Alan Sabol, Jonathan Martinez, Kenneth Shadomy, Sean Cymet, Tyler Emanuel, Peter TI Multigeneration Cross-Contamination of Mail with Bacillus anthracis Spores SO PLOS ONE LA English DT Article ID BIOTERRORISM-RELATED ANTHRAX; FATAL INHALATIONAL ANTHRAX; UNITED-STATES; OUTBREAK; CONNECTICUT; WASHINGTON; DC AB The release of biological agents, including those which could be used in biowarfare or bioterrorism in large urban areas, has been a concern for governments for nearly three decades. Previous incidents from Sverdlosk and the postal anthrax attack of 2001 have raised questions on the mechanism of spread of Bacillus anthracis spores as an aerosol or contaminant. Prior studies have demonstrated that Bacillus atrophaeus is easily transferred through simulated mail handing, but no reports have demonstrated this ability with Bacillus anthracis spores, which have morphological differences that may affect adhesion properties between spore and formite. In this study, equipment developed to simulate interactions across three generations of envelopes subjected to tumbling and mixing was used to evaluate the potential for cross-contamination of B. anthracis spores in simulated mail handling. In these experiments, we found that the potential for cross-contamination through letter tumbling from one generation to the next varied between generations while the presence of a fluidizer had no statistical impact on the transfer of material. Likewise, the presence or absence of a fluidizer had no statistically significant impact on cross-contamination levels or reaerosolization from letter opening. C1 [Edmonds, Jason; Sabol, Jonathan; Emanuel, Peter] US Army, Dept Def, Edgewood Chem Biol Ctr, 5183 Blackhawk Rd, Aberdeen Proving Ground, MD 21010 USA. [Lindquist, H. D. Alan] US EPA, Off Res & Dev, Natl Homeland Secur Res Ctr, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. [Martinez, Kenneth] HWC Inc, 1100 New York Ave NW, Washington, DC 20005 USA. [Shadomy, Sean] Ctr Dis Control & Prevent, Natl Ctr Emerging & Zoonot Infect Dis, 1600 Clifton Rd,MS A-30, Atlanta, GA 30333 USA. [Cymet, Tyler] Amer Assoc Coll Osteopath Med, Med Educ, Chevy Chase, MD 20815 USA. RP Edmonds, J (reprint author), US Army, Dept Def, Edgewood Chem Biol Ctr, 5183 Blackhawk Rd, Aberdeen Proving Ground, MD 21010 USA. EM jason.m.edmonds2.civ@mail.mil FU Combatting Terrorism Technical Support Working Group, Caution; Hassett Willis; Department of Defense; Edgewood Chemical Biological Center; Department of Health and Human Services Centers for Disease Control and Prevention; National Institutes for Occupational Safety and Health; Department of Justice; Federal Bureau of Investigations; U.S. Environmental Protection Agency through its Office of Research and Development FX This program was partially funded by the Technical Support Working Group, now the Combatting Terrorism Technical Support Working Group, Caution (http://www.cttso.gov/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Hassett Willis provided support in the form of salaries for authors (KM), but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The Department of Defense, Edgewood Chemical Biological Center, Department of Health and Human Services Centers for Disease Control and Prevention, National Institutes for Occupational Safety and Health, Department of Justice, Federal Bureau of Investigations, and the U.S. Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here. This manuscript has been subject to Agency review and but does not necessarily reflect the views of the Agencies listed above. The specific roles of these authors are articulated in the author contributions section.; The Department of Defense, Edgewood Chemical Biological Center, Department of Health and Human Services Centers for Disease Control and Prevention, National Institutes for Occupational Safety and Health, Department of Justice, Federal Bureau of Investigations, and the U.S. Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here. This manuscript has been subject to Agency review and but does not necessarily reflect the views of the Agencies listed above. No official endorsement should be inferred. Mention or use of trade names does not constitute endorsement of recommendation for use. NR 25 TC 0 Z9 0 U1 3 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 28 PY 2016 VL 11 IS 4 AR e0152225 DI 10.1371/journal.pone.0152225 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DK8XE UT WOS:000375211700004 PM 27123934 ER PT J AU Wang, XT Campbell, MR Lacher, SE Cho, HY Wan, M Crowl, CL Chorley, BN Bond, GL Kleeberger, SR Slattery, M Bell, DA AF Wang, Xuting Campbell, Michelle R. Lacher, Sarah E. Cho, Hye-Youn Wan, Ma Crowl, Christopher L. Chorley, Brian N. Bond, Gareth L. Kleeberger, Steven R. Slattery, Matthew Bell, Douglas A. TI A Polymorphic Antioxidant Response Element Links NRF2/sMAF Binding to Enhanced MAPT Expression and Reduced Risk of Parkinsonian Disorders SO CELL REPORTS LA English DT Article ID GENE-EXPRESSION; HUMAN GENOME; NEURODEGENERATIVE DISEASES; ALZHEIMERS-DISEASE; CONSENSUS SEQUENCE; COMMON VARIANTS; IDENTIFICATION; NRF2; DNA; TAU AB The NRF2/sMAF protein complex regulates the oxidative stress response by occupying cis-acting enhancers containing an antioxidant response element (ARE). Integrating genome-wide maps of NRF2/sMAF occupancy with disease-susceptibility loci, we discovered eight polymorphic AREs linked to 14 highly ranked disease-risk SNPs in individuals of European ancestry. Among these SNPs was rs242561, located within a regulatory region of the MAPT gene (encoding microtubule-associated protein Tau). It was consistently occupied by NRF2/sMAF in multiple experiments and its strong-binding allele associated with higher mRNA levels in cell lines and human brain tissue. Induction of MAPT transcription by NRF2 was confirmed using a human neuroblastoma cell line and a Nrf2-deficient mouse model. Most importantly, rs242561 displayed complete linkage disequilibrium with a highly protective allele identified in multiple GWASs of progressive supranuclear palsy, Parkinson's disease, and corticobasal degeneration. These observations suggest a potential role for NRF2/sMAF in tauopathies and a possible role for NRF2 pathway activators in disease prevention. C1 [Wang, Xuting; Campbell, Michelle R.; Wan, Ma; Crowl, Christopher L.; Chorley, Brian N.; Bell, Douglas A.] Natl Inst Environm Hlth Sci, Environm Genom Sect, Genome Integr & Struct Biol Lab, NIH, Res Triangle Pk, NC 27709 USA. [Lacher, Sarah E.; Slattery, Matthew] Univ Minnesota, Sch Med, Dept Biomed Sci, Duluth, MN 55812 USA. [Lacher, Sarah E.; Slattery, Matthew] Univ Minnesota, Ctr Dev Biol, Minneapolis, MN 55455 USA. [Cho, Hye-Youn; Kleeberger, Steven R.] Natl Inst Environm Hlth Sci, Immun Inflammat & Dis Lab, NIH, Res Triangle Pk, NC 27709 USA. [Bond, Gareth L.] Univ Oxford, Nuffield Dept Clin Med, Ludwig Inst Canc Res, Old Rd Campus Res Bldg, Oxford OX3 7DQ, England. [Chorley, Brian N.] US Environm Protect Agcy, Res Triangle Pk, NC 27709 USA. RP Wang, XT; Bell, DA (reprint author), Natl Inst Environm Hlth Sci, Environm Genom Sect, Genome Integr & Struct Biol Lab, NIH, Res Triangle Pk, NC 27709 USA. EM wang21@niehs.nih.gov; bell1@niehs.nih.gov OI Wang, Xuting/0000-0001-6781-8008 FU Intramural Research Program of National Institute of Environmental Health Sciences, NIH [Z01ES100475, Z01ES46008]; University of Minnesota Foundation; Ludwig Institute for Cancer Research FX This work was funded by the Intramural Research Program of National Institute of Environmental Health Sciences, NIH (projects: Z01ES100475 and Z01ES46008), the University of Minnesota Foundation (to M.S.), and the Ludwig Institute for Cancer Research (to G.L.B.). The authors would like to acknowledge Dr. Shuangshuang Dai for Linux computing support; NIEHS Epigenomics Core; NIEHS Animal Facility Core; and Dr. Jean Harry, NIEHS, for technical advice and useful comments on the manuscript. NR 53 TC 3 Z9 3 U1 2 U2 2 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 2211-1247 J9 CELL REP JI Cell Reports PD APR 26 PY 2016 VL 15 IS 4 BP 830 EP 842 DI 10.1016/j.celrep.2016.03.068 PG 13 WC Cell Biology SC Cell Biology GA DK2FS UT WOS:000374730600015 ER PT J AU Yu, SC Li, PF Wang, LQ Wang, P Wang, S Chang, SC Liu, WP Alapaty, K AF Yu, Shaocai Li, Pengfei Wang, Liqiang Wang, Peng Wang, Si Chang, Shucheng Liu, Weiping Alapaty, Kiran TI Anthropogenic aerosols are a potential cause for migration of the summer monsoon rain belt in China SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Letter C1 [Yu, Shaocai; Li, Pengfei; Wang, Liqiang; Wang, Peng; Wang, Si; Chang, Shucheng; Liu, Weiping] Zhejiang Univ, Coll Environm & Resource Sci, Key Lab Environm Remediat & Ecol Hlth, Minist Educ, Hangzhou 310058, Zhejiang, Peoples R China. [Yu, Shaocai; Li, Pengfei; Wang, Liqiang; Wang, Peng; Wang, Si; Chang, Shucheng; Liu, Weiping] Zhejiang Univ, Coll Environm & Resource Sci, Res Ctr Air Pollut & Hlth, Hangzhou 310058, Zhejiang, Peoples R China. [Alapaty, Kiran] US EPA, Syst Exposure Div, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. RP Yu, SC (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, Key Lab Environm Remediat & Ecol Hlth, Minist Educ, Hangzhou 310058, Zhejiang, Peoples R China.; Yu, SC (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, Res Ctr Air Pollut & Hlth, Hangzhou 310058, Zhejiang, Peoples R China.; Alapaty, K (reprint author), US EPA, Syst Exposure Div, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. EM shaocaiyu@zju.edu.cn; alapaty.kiran@epa.gov RI yu, shaocai/F-1394-2014; Liu, Weiping/E-9208-2010 OI Liu, Weiping/0000-0002-1173-892X NR 6 TC 5 Z9 5 U1 16 U2 32 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 19 PY 2016 VL 113 IS 16 BP E2209 EP E2210 DI 10.1073/pnas.1601104113 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ7LV UT WOS:000374393800001 PM 27006506 ER PT J AU Holder, AL Hagler, GSW Aurell, J Hays, MD Gullett, BK AF Holder, Amara L. Hagler, Gayle S. W. Aurell, Johanna Hays, Michael D. Gullett, Brian K. TI Particulate matter and black carbon optical properties and emission factors from prescribed fires in the southeastern United States SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE biomass burning; black carbon mixing state; aerosol optical properties; size distributions; brown carbon ID BIOMASS BURNING PARTICLES; TRACE GASES; SIZE DISTRIBUTIONS; FIELD-MEASUREMENTS; LIGHT-ABSORPTION; ORGANIC-CARBON; BROWN CARBON; SMOKE; AEROSOLS; FOREST AB Aerosol optical properties of biomass burning emissions are critical parameters determining how these emissions impact the Earth's climate. Despite their importance, field measurements of aerosol optical properties from fires remain scarce. Aerosol emissions from prescribed fires of forested and grass plots in the southeastern United States were measured and compared to emissions from laboratory simulations. Fine particulate matter (PM2.5), black carbon (BC), and aerosol light scattering and absorption were characterized for all fires. Refractory BC emission factors (EFs) measured at ground level (similar to 2m) were 0.760.15g/kg, comparable to the 0.930.32g/kg measured aloft (similar to 100-600m). However, PM EFs measured by aircraft were only 18% (5.42.0g/kg) of those measured on the ground (28.89.8g/kg). Such large differences in PM EFs for the same fire have not been previously reported and may plausibly be due to the differing particle measurement methodologies being applied but also likely related to partitioning of organic compounds to the gas phase as the plume dilutes aloft. Higher PM EFs on the ground may also be related to a higher contribution from smoldering combustion. The absorption angstrom ngstrom exponents ((a)) for the high intensity South Carolina fires were 3.92 +/- 0.6, which was larger than prescribed forest fire in Florida (2.84) and the grass fire in Florida (2.71), implying a larger absorption contribution from brown carbon from higher-intensity fires. Aerosol optical properties from laboratory simulations did not represent field measurements. C1 [Holder, Amara L.; Hagler, Gayle S. W.; Hays, Michael D.; Gullett, Brian K.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Aurell, Johanna] Univ Dayton, Res Inst, Energy Technol & Mat Div, Dayton, OH 45469 USA. RP Holder, AL (reprint author), US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. EM holder.amara@epa.gov FU U. S. Environmental Protection Agency; Strategic Environmental Research and Development Program (SERDP) FX This work was funded by the U. S. Environmental Protection Agency and in part by the Strategic Environmental Research and Development Program (SERDP). We would like to acknowledge Bill Squire, Bill Mitchell, and Chris Pressley of the U. S. Environmental Protection Agency for their assistance in field measurements and Steve Terll (ARCADIS-US) for assistance with laboratory measurements. We would like to thank Roger Ottmar and David Weise (US Forest Service) and Tim Johnson (PNNL) for coordinating this multigroup research effort. We would also like to thank John Maitland and Stanley Rikard at Fort Jackson (SC) and Kevin Hiers and Brett Williams at Eglin Air Force Base (FL). Data used in the writing of this manuscript can be obtained upon request to Amara Holder (holder.amara@epa.gov). The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U. S. Environmental Protection Agency. NR 73 TC 2 Z9 2 U1 12 U2 31 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD APR 16 PY 2016 VL 121 IS 7 BP 3465 EP 3483 DI 10.1002/2015JD024321 PG 19 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DK7QG UT WOS:000375120200024 ER PT J AU Moser, VC Padilla, S AF Moser, Virginia C. Padilla, Stephanie TI Esterase detoxication of acetylcholinesterase inhibitors using human liver samples in vitro SO TOXICOLOGY LA English DT Article DE Acetylcholinesterase inhibitor; PON1; Carboxylesterase; Organophosphate; Carbamate; In vitro; Human ID HUMAN-SERUM PARAOXONASE; ORGANOPHOSPHORUS COMPOUNDS; CHLORPYRIFOS-OXON; PON1 ACTIVITY; INTERINDIVIDUAL VARIABILITY; HUMAN CARBOXYLESTERASES; METHYL PARAOXON; A-ESTERASES; ADULT RATS; TOXICITY AB Organophosphorus (OP) and N-methylcarbamate pesticides inhibit acetylcholinesterase (AChE), but differences in metabolism and detoxication can influence potency of these pesticides across and within species. Carboxylesterase (CaE) and A-esterase (paraoxonase, PON1) are considered factors underlying age-related sensitivity differences. We used an in vitro system to measure detoxication of AChE-inhibiting pesticides mediated via these esterases. Recombinant human AChE was used as a bioassay of inhibitor concentration following incubation with detoxifying tissue: liver plus Ca+2 (to stimulate PON1s, measuring activity of both esterases) or EGTA (to inhibit PON1s, thereby measuring CaE activity). AChE inhibitory concentrations of aldicarb, chlorpyrifos oxon, malaoxon, methamidophos, oxamyl, paraoxon, and methylparaoxon were incubated with liver homogenates from adult male rat or one of 20 commercially provided human (11-83 years of age) liver samples. Detoxication was defined as the difference in inhibition produced by the pesticide alone and inhibition measured in combination with liver plus Ca+2 or liver plus EGTA. Generally, rat liver produced more detoxication than did the human samples. There were large detoxication differences across human samples for some pesticides (especially malaoxon, chlorpyrifos oxon) but not for others (e.g., aldicarb, methamidophos); for the most part these differences did not correlate with age or sex. Chlorpyrifos oxon was fully detoxified only in the presence of Ce+2 in both rat and human livers. Detoxication of paraoxon and methylparaoxon in rat liver was greater with Ca+2, but humans showed less differentiation than rats between Ca+2 and EGTA conditions. This suggests the importance of PON1 detoxication for these three OPs in the rat, but mostly only for chlorpyrifos oxon in human samples. Malaoxon was detoxified similarly with Ca+2 or EGTA, and the differences across humans correlated with metabolism of p-nitrophenyl acetate, a substrate for CaEs. This suggests the importance of CaEs in malaoxon detoxication. Understanding these individual differences in detoxication can inform human variability in pesticide sensitivity. Published by Elsevier Ireland Ltd. C1 [Moser, Virginia C.] US EPA, Toxic Assessment Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Padilla, Stephanie] US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Moser, VC (reprint author), Toxic Assessment Div MD B105 04, 109 TW Alexander Dr US EPA, Res Triangle Pk, NC 27711 USA. EM Moser.ginger@epa.gov FU Office of Research and Development, US EPA FX The authors have no conflict of interest. The research was funded via the intramural research program of the Office of Research and Development, US EPA. NR 69 TC 0 Z9 0 U1 10 U2 18 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PD APR 15 PY 2016 VL 353 BP 11 EP 20 DI 10.1016/j.tox.2016.04.006 PG 10 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA DO4DL UT WOS:000377731900002 PM 27132127 ER PT J AU Gavett, SH Parkinson, CU Willson, GA Wood, CE Jarabek, AM Roberts, KC Kodavanti, UP Dodd, DE AF Gavett, Stephen H. Parkinson, Carl U. Willson, Gabrielle A. Wood, Charles E. Jarabek, Annie M. Roberts, Kay C. Kodavanti, Urmila P. Dodd, Darol E. TI Persistent effects of Libby amphibole and amosite asbestos following subchronic inhalation in rats SO PARTICLE AND FIBRE TOXICOLOGY LA English DT Article DE Asbestos; Libby amphibole; Amosite; Inhalation; Inflammation; Fibrosis; Adenoma; Carcinoma; Dosimetry; Risk assessment ID SHORT-TERM INHALATION; ACUTE-PHASE RESPONSE; TNF-ALPHA; IN-VITRO; NEUTROPHIL RECRUITMENT; RADIOGRAPHIC CHANGES; VERMICULITE MINERS; NALP3 INFLAMMASOME; EXPOSURE; LUNG AB Background: Human exposure to Libby amphibole (LA) asbestos increases risk of lung cancer, mesothelioma, and non-malignant respiratory disease. This study evaluated potency and time-course effects of LA and positive control amosite (AM) asbestos fibers in male F344 rats following nose-only inhalation exposure. Methods: Rats were exposed to air, LA (0.5, 3.5, or 25.0 mg/m(3) targets), or AM (3.5 mg/m(3) target) for 10 days and assessed for markers of lung inflammation, injury, and cell proliferation. Short-term results guided concentration levels for a stop-exposure study in which rats were exposed to air, LA (1.0, 3.3, or 10.0 mg/m(3)), or AM (3.3 mg/m(3)) 6 h/day, 5 days/week for 13 weeks, and assessed 1 day, 1, 3, and 18 months post-exposure. Fibers were relatively short; for 10 mg/m(3) LA, mean length of all structures was 3.7 mu m and 1 % were longer than 20 mu m. Results: Ten days exposure to 25.0 mg/m3 LA resulted in significantly increased lung inflammation, fibrosis, bronchiolar epithelial cell proliferation and hyperplasia, and inflammatory cytokine gene expression compared to air. Exposure to 3.5 mg/m3 LA resulted in modestly higher markers of acute lung injury and inflammation compared to AM. Following 13 weeks exposure, lung fiber burdens correlated with exposure mass concentrations, declining gradually over 18 months. LA (3.3 and 10.0 mg/m(3)) and AM produced significantly higher bronchoalveolar lavage markers of inflammation and lung tissue cytokines, Akt, and MAPK/ERK pathway components compared to air control from 1 day to 3 months post-exposure. Histopathology showed alveolar inflammation and interstitial fibrosis in all fiber-exposed groups up to 18 months post-exposure. Positive dose trends for incidence of alveolar epithelial hyperplasia and bronchiolar/alveolar adenoma or carcinoma were observed among LA groups. Conclusions: Inhalation of relatively short LA fibers produced inflammatory, fibrogenic, and tumorigenic effects in rats which replicate essential attributes of asbestos-related disease in exposed humans. Fiber burden, inflammation, and activation of growth factor pathways may persist and contribute to lung tumorigenesis long after initial LA exposure. Fiber burden data are being used to develop a dosimetry model for LA fibers, which may provide insights on mode of action for hazard assessment. C1 [Gavett, Stephen H.; Wood, Charles E.; Kodavanti, Urmila P.] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Parkinson, Carl U.; Roberts, Kay C.; Dodd, Darol E.] Hamner Inst Hlth Sci, Res Triangle Pk, NC 27711 USA. [Willson, Gabrielle A.] Expt Pathol Labs Inc EPL, Res Triangle Pk, NC 27711 USA. [Jarabek, Annie M.] US EPA, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. RP Gavett, SH (reprint author), US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. EM Gavett.Stephen@epa.gov FU U.S. Environmental Protection Agency; Hamner Institutes for Health Sciences [EP-W-08-051]; U.S. EPA FX The research described in this article has been funded by the U.S. Environmental Protection Agency in a contract with the Hamner Institutes for Health Sciences (EP-W-08-051) and by U.S. EPA internal funding. NR 69 TC 1 Z9 1 U1 6 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-8977 J9 PART FIBRE TOXICOL JI Part. Fibre Toxicol. PD APR 15 PY 2016 VL 13 AR 17 DI 10.1186/s12989-016-0130-z PG 20 WC Toxicology SC Toxicology GA DK1MD UT WOS:000374675800001 PM 27083413 ER PT J AU Ward-Caviness, CK Neas, LM Blach, C Haynes, CS LaRocque-Abramson, K Grass, E Dowdy, E Devlin, RB Diaz-Sanchez, D Cascio, WE Miranda, ML Gregory, SG Shah, SH Kraus, WE Hauser, ER AF Ward-Caviness, Cavin K. Neas, Lucas M. Blach, Colette Haynes, Carol S. LaRocque-Abramson, Karen Grass, Elizabeth Dowdy, Elaine Devlin, Robert B. Diaz-Sanchez, David Cascio, Wayne E. Miranda, Marie Lynn Gregory, Simon G. Shah, Svati H. Kraus, William E. Hauser, Elizabeth R. TI Genetic Variants in the Bone Morphogenic Protein Gene Family Modify the Association between Residential Exposure to Traffic and Peripheral Arterial Disease SO PLOS ONE LA English DT Article ID GENOME-WIDE ASSOCIATION; CORONARY-HEART-DISEASE; HEINZ-NIXDORF RECALL; AIR-POLLUTION; DNA METHYLATION; VASCULAR CALCIFICATION; CARDIOVASCULAR-DISEASE; HUMAN TRANSCRIPTOME; RISK-FACTOR; ATHEROSCLEROSIS AB There is a growing literature indicating that genetic variants modify many of the associations between environmental exposures and clinical outcomes, potentially by increasing susceptibility to these exposures. However, genome-scale investigations of these interactions have been rarely performed particularly in the case of air pollution exposures. We performed race-stratified genome-wide gene-environment interaction association studies on European-American (EA, N = 1623) and African-American (AA, N = 554) cohorts to investigate the joint influence of common single nucleotide polymorphisms (SNPs) and residential exposure to traffic ("traffic exposure")-a recognized vascular disease risk factor-on peripheral arterial disease (BMP8A eQTLs in tissue types highlight relevant for PAD such as rs755249 (tibial nerve, eQTL P = 3.6x10(-6)) and rs1180341 (tibial artery, eQTL P = 5.3x10(-6)). Together these results reveal a novel gene, and possibly gene family, associated with PAD via an interaction with traffic air pollution exposure. These results also highlight the potential for interactions studies, particularly at the genome scale, to reveal novel biology linking environmental exposures to clinical outcomes.PAD). Traffic exposure was estimated via the distance from the primary residence to the nearest major roadway, defined as the nearest limited access highways or major arterial. The rs755249-traffic exposure interaction was associated with PAD at a genome-wide significant level (P = 2.29x10(-8)) in European-Americans. Rs755249 is located in the 3' untranslated region of BMP8A, a member of the bone morphogenic protein (BMP) gene family. Further investigation revealed several variants in BMP genes associated with PAD via an interaction with traffic exposure in both the EA and AA cohorts; this included interactions with non-synonymous variants in BMP2, which is regulated by air pollution exposure. The BMP family of genes is linked to vascular growth and calcification and is a novel gene family for the study of PAD pathophysiology. Further investigation of BMP8A using the Genotype Tissue Expression Database revealed multiple variants with nominally significant (P < 0.05) interaction P-values in our EA cohort were significant BMP8A eQTLs in tissue types highlight relevant for PAD such as rs755249 (tibial nerve, eQTL P = 3.6x10(-6)) and rs1180341 (tibial artery, eQTL P = 5.3x10(-6)). Together these results reveal a novel gene, and possibly gene family, associated with PAD via an interaction with traffic air pollution exposure. These results also highlight the potential for interactions studies, particularly at the genome scale, to reveal novel biology linking environmental exposures to clinical outcomes. C1 [Ward-Caviness, Cavin K.; Blach, Colette; Haynes, Carol S.; LaRocque-Abramson, Karen; Grass, Elizabeth; Dowdy, Elaine; Gregory, Simon G.; Shah, Svati H.; Kraus, William E.; Hauser, Elizabeth R.] Duke Univ, Med Ctr, Duke Mol Physiol Inst, Durham, NC 27710 USA. [Ward-Caviness, Cavin K.] Helmholtz Ctr Munich, Inst Epidemiol 2, D-85764 Neuherberg, Germany. [Neas, Lucas M.; Devlin, Robert B.; Diaz-Sanchez, David; Cascio, Wayne E.] US Environm Protect Agcy, Natl Hlth & Environm Effects Res Lab, Chapel Hill, NC 27514 USA. [Miranda, Marie Lynn] Rice Univ, Natl Ctr Geospatial Med, Houston, TX 77005 USA. [Shah, Svati H.; Kraus, William E.] Duke Univ, Med Ctr, Div Cardiovasc Med, Durham, NC 27710 USA. [Hauser, Elizabeth R.] Duke Univ, Med Ctr, Dept Biostat & Bioinformat, Durham, NC 27710 USA. [Hauser, Elizabeth R.] Vet Affairs Med Ctr, Cooperat Studies Program, Epidemiol Ctr Durham, Durham, NC 27701 USA. RP Hauser, ER (reprint author), Duke Univ, Med Ctr, Duke Mol Physiol Inst, Durham, NC 27710 USA.; Hauser, ER (reprint author), Duke Univ, Med Ctr, Dept Biostat & Bioinformat, Durham, NC 27710 USA.; Hauser, ER (reprint author), Vet Affairs Med Ctr, Cooperat Studies Program, Epidemiol Ctr Durham, Durham, NC 27701 USA. EM elizabeth.hauser@gmail.com FU NIH [HL095987, HL10621, HL36587]; United States Environmental Protection Agency (EPA) [R-82811201] FX This work was supported by the following NIH grants: HL095987, HL10621, and HL36587. This project was supported in part by an appointment to the Research Participation Program at the Office of Research and Development, U.S. Environmental Protection Agency. Research described in this article was conducted under contract to the Health Effects Institute (HEI), and organization jointly funded by the United States Environmental Protection Agency (EPA) (Assistance Award No. R-82811201) and certain motor vehicle and engine manufacturers. The contents of this article do not necessarily reflect the views of HEI, or its sponsors, nor do they necessarily reflect the view and policies of the EPA or motor vehicle and engine manufacturers.; This work was supported by the following NIH grants: HL095987, HL10621, and HL36587. This project was supported in part by an appointment to the Research Participation Program at the Office of Research and Development, U.S. Environmental Protection Agency. The views expressed in this article are those of the authors and do not necessarily reflect the views or policies of the EPA. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. The contents of this article do not necessarily reflect the views of the Health Effects Institute (HEI), an organization jointly funded by the United States Environmental Protection Agency (EPA) (Assistance Award No. R-82811201) and certain motor vehicle and engine manufacturers, nor do they necessarily reflect the views and policies of its sponsors. NR 72 TC 0 Z9 0 U1 1 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 15 PY 2016 VL 11 IS 4 AR e0152670 DI 10.1371/journal.pone.0152670 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ6AH UT WOS:000374291800007 PM 27082954 ER PT J AU Hill, BH Jicha, TM Lehto, LLP Elonen, CM Sebestyen, SD Kolka, RK AF Hill, Brian H. Jicha, Terri M. Lehto, LaRae L. P. Elonen, Colleen M. Sebestyen, Stephen D. Kolka, Randall K. TI Comparisons of soil nitrogen mass balances for an ombrotrophic bog and a minerotrophic fen in northern Minnesota SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Bog; Denitrification; Fen; Peat; Soils; Watershed N budget ID FRESH-WATER WETLANDS; ORGANIC-MATTER; ACETYLENE INHIBITION; FORESTED WATERSHEDS; AQUATIC ECOSYSTEMS; CARBON-CYCLE; PORE-WATER; PEAT BOG; DENITRIFICATION; PEATLANDS AB We compared nitrogen (N) storage and flux in soils from an ombrotrophic bog with that of a minerotrophic ten to quantity the differences in N cycling between these two peatlands types in northern Minnesota (USA). Precipitation, atmospheric deposition, and bog and ten outflows were analyzed for nitrogen species. Upland and peatland soil samples were analyzed for N content, and for ambient (DN) and potential (DEA) denitrification rates. Annual atmospheric deposition was: 0.88-3.07 kg NH4+ ha(-1) y(-1); 1.37-1.42 kg NO3- ha(-1) y(-1); 2.79-4.69 kg TN ha(-1) y(-1). Annual N outflows were: bog -0.01-0.04 kg NH4+ ha(-1) y(-1), NO3- 0.01-0.06 kg ha(-1) y(-1) and TN 0.11-0.69 kg ha(-1) y(-1); fen-NH4+ 0.01-0.16 kg ha(-1) y(-1), NO3- 0.29-0.48 kg ha(-1) y(-1), and TN 1.14-1.61 kg ha(-1) y(-1). Soil N content depended on location within the bog or fen, and on soil depth. DN and DEA rates were low throughout the uplands and peatlancls, and were correlated with atmospheric N deposition, soil N storage, and N outflow. DEA was significantly greater than DN indicating C or N limitation of the denitrification process. We highlight differences between the bog and fen, between the upland mineral soils and peat, and the importance of biogeochemical hotspots within the peatlands. We point out the importance of organic N storage, as a source of N for denitrification, and propose a plausible link between organic N storage, denitrification and N export from peatlands. Finally, we considered the interactions of microbial metabolism with nutrient availability and stoichiometry, and how N dynamics might be affected by climate change in peatland ecosystems. Published by Elsevier B.V. C1 [Hill, Brian H.; Jicha, Terri M.; Lehto, LaRae L. P.; Elonen, Colleen M.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Lab, Midcontinent Ecol Div, Duluth, MN 55804 USA. [Sebestyen, Stephen D.; Kolka, Randall K.] US Forest Serv, No Res Stn, Ctr Res Ecosyst Change, Grand Rapids, MN 55744 USA. [Lehto, LaRae L. P.] AMI Consulting Engineers, Superior, WI USA. RP Hill, BH (reprint author), USEPA NHEERL MED, 6201 Congdon Blvd, Duluth, MN 55804 USA. EM hill.brian@epa.gov RI Sebestyen, Stephen/D-1238-2013 OI Sebestyen, Stephen/0000-0002-6315-0108 NR 60 TC 2 Z9 2 U1 8 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD APR 15 PY 2016 VL 550 BP 880 EP 892 DI 10.1016/j.scitotenv.2016.01.178 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DF3EM UT WOS:000371226700087 PM 26851760 ER PT J AU Wong, K Shaw, TI Oladeinde, A Glenn, TC Oakley, B Molina, M AF Wong, Kelvin Shaw, Timothy I. Oladeinde, Adelumola Glenn, Travis C. Oakley, Brian Molina, Marirosa TI Rapid Microbiome Changes in Freshly Deposited Cow Feces under Field Conditions SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE metagenomics; cattle feces; microbiome changes; oxygen exposure; sunlight exposure; fecal contamination ID DISSOLVED ORGANIC-MATTER; RIBOSOMAL-RNA SEQUENCES; BACTERIAL-GROWTH; SP NOV.; PATHOGENIC BACTERIA; COMMUNITY ANALYSIS; SOURCE-TRACKING; BOVINE MANURE; UV-RADIATION; GEN. NOV. AB Although development of next generation sequencing (NGS) has substantially improved our understanding of the microbial ecology of animal feces, previous studies have mostly focused on freshly excreted feces. There is still limited understanding of the aging process dynamics of fecal microbiomes in intact cowpats exposed to natural environments. Fresh cowpats were sampled at multiple time points for 57 days under field conditions; half the samples were exposed to sunlight (unshaded) while the other half was protected from sunlight (shaded). The 16SRNA hypervariable region 4 was amplified from each sample and sequenced on an Illumina MiSeq Platform. While Clostridia, Bacteroidia, and Sphingobacteria were dominant classes of bacteria in fresh cowpats, Alphaproteobacteria, Betaproteobacteria, Actinobacteria, and Bacilli were the dominant classes by the end of the study, indicating a general shift from anaerobic to aerobic bacterial populations. This change was most likely influenced by the shift from cattle gut (anaerobic) to pasture ground (aerobic). Reduced moisture in cowpats may also contribute to the community shift since air can penetrate the dryer cowpat more easily. Twelve genera consisting pathogenic bacteria were detected, with Mycobacterium, Bacillus, and Clostridium being the most abundant; their combined abundance accounts for 90% of the total pathogenic genera. Taxonomic richness and diversity increased throughout the study for most samples, which could be due to bacteria regrowth and colonization of bacteria from the environment. In contrast to the high taxonomic diversity, the changes of PICRUSt inferred function profile were minimal for all cowpats throughout the study, which suggest that core functions predicted by PICRUSt may be too conserved to distinguish differences between aerobe and anaerobe. To the best of our knowledge, this is the first study demonstrating that cowpat exposure to air and sunlight can cause drastic microbiome changes soon after deposition in natural environments. Our findings offer important insights for future research characterizing the microbiome of feces collected in natural environments and the impact of cattle fecal contamination on water resources. C1 [Wong, Kelvin; Oladeinde, Adelumola; Molina, Marirosa] US EPA, Ecosyst Res Div, Athens, GA USA. [Wong, Kelvin] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Shaw, Timothy I.] Univ Georgia, Inst Bioinformat, Athens, GA 30602 USA. [Shaw, Timothy I.] St Jude Childrens Res Hosp, Dept Computat Biol, 332 N Lauderdale St, Memphis, TN 38105 USA. [Oladeinde, Adelumola; Glenn, Travis C.] Univ Georgia, Dept Environm Hlth Sci, Athens, GA 30602 USA. [Oakley, Brian] Western Univ Hlth Sci, Coll Vet Med, Pomona, CA USA. RP Molina, M (reprint author), US EPA, Ecosyst Res Div, Athens, GA USA. EM molina.marirosa@epa.gov NR 45 TC 0 Z9 0 U1 9 U2 21 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD APR 13 PY 2016 VL 7 AR 500 DI 10.3389/fmicb.2016.00500 PG 12 WC Microbiology SC Microbiology GA DJ1RE UT WOS:000373980200001 PM 27148189 ER PT J AU Simkin, SM Allen, EB Bowman, WD Clark, CM Belnap, J Brooks, ML Cade, BS Collins, SL Geiser, LH Gilliam, FS Jovan, SE Pardo, LH Schulz, BK Stevens, CJ Suding, KN Throop, HL Waller, DM AF Simkin, Samuel M. Allen, Edith B. Bowman, William D. Clark, Christopher M. Belnap, Jayne Brooks, Matthew L. Cade, Brian S. Collins, Scott L. Geiser, Linda H. Gilliam, Frank S. Jovan, Sarah E. Pardo, Linda H. Schulz, Bethany K. Stevens, Carly J. Suding, Katharine N. Throop, Heather L. Waller, Donald M. TI Conditional vulnerability of plant diversity to atmospheric nitrogen deposition across the United States SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE nitrogen deposition; plant species richness; diversity; soil pH; climate ID SPECIES RICHNESS; NUTRIENT ENRICHMENT; ECOSYSTEM RESPONSE; BIODIVERSITY LOSS; CRITICAL LOADS; VEGETATION; GRASSLANDS; EUTROPHICATION; TERRESTRIAL; COMMUNITIES AB Atmospheric nitrogen (N) deposition has been shown to decrease plant species richness along regional deposition gradients in Europe and in experimental manipulations. However, the general response of species richness to N deposition across different vegetation types, soil conditions, and climates remains largely unknown even though responses may be contingent on these environmental factors. We assessed the effect of N deposition on herbaceous richness for 15,136 forest, woodland, shrubland, and grassland sites across the continental United States, to address how edaphic and climatic conditions altered vulnerability to this stressor. In our dataset, with N deposition ranging from 1 to 19 kg N.ha(-1).y(-1), we found a unimodal relationship; richness increased at low deposition levels and decreased above 8.7 and 13.4 kg N.ha(-1).y(-1) in open and closed-canopy vegetation, respectively. N deposition exceeded critical loads for loss of plant species richness in 24% of 15,136 sites examined nationwide. There were negative relationships between species richness and N deposition in 36% of 44 community gradients. Vulnerability to N deposition was consistently higher in more acidic soils whereas the moderating roles of temperature and precipitation varied across scales. We demonstrate here that negative relationships between N deposition and species richness are common, albeit not universal, and that fine-scale processes can moderate vegetation responses to N deposition. Our results highlight the importance of contingent factors when estimating ecosystem vulnerability to N deposition and suggest that N deposition is affecting species richness in forested and nonforested systems across much of the continental United States. C1 [Simkin, Samuel M.; Bowman, William D.; Suding, Katharine N.] Univ Colorado, Inst Arctic & Alpine Res, Boulder, CO 80309 USA. [Simkin, Samuel M.; Bowman, William D.; Suding, Katharine N.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Allen, Edith B.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Allen, Edith B.] Univ Calif Riverside, Ctr Conservat Biol, Riverside, CA 92521 USA. [Clark, Christopher M.] US EPA, Natl Ctr Environm Assessment, Washington, DC 20460 USA. [Belnap, Jayne] US Geol Survey, Southwest Biol Sci Ctr, Moab, UT 84532 USA. [Brooks, Matthew L.] US Geol Survey, Western Ecol Res Ctr, Oakhurst, CA 93644 USA. [Cade, Brian S.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80226 USA. [Collins, Scott L.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Geiser, Linda H.] US Forest Serv, Pacific Northwest Reg Air Resource Management Pro, USDA, Corvallis, OR 97339 USA. [Gilliam, Frank S.] Marshall Univ, Dept Biol Sci, Huntington, WV 25755 USA. [Jovan, Sarah E.] US Forest Serv, Forest Inventory & Anal Program, USDA, Portland, OR 97339 USA. [Pardo, Linda H.] US Forest Serv, No Res Stn, USDA, Burlington, VT 05405 USA. [Schulz, Bethany K.] US Forest Serv, Forest Inventory & Anal Program, USDA, Anchorage, AK 99501 USA. [Stevens, Carly J.] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Throop, Heather L.] Arizona State Univ, Sch Earth & Space Explorat, Tempe, AZ 85287 USA. [Throop, Heather L.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Waller, Donald M.] Univ Wisconsin, Dept Bot, Madison, WI 53706 USA. RP Simkin, SM (reprint author), Univ Colorado, Inst Arctic & Alpine Res, Boulder, CO 80309 USA.; Simkin, SM (reprint author), Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. EM samuel.simkin@colorado.edu RI Collins, Scott/P-7742-2014; Throop, Heather/D-6391-2012 OI Collins, Scott/0000-0002-0193-2892; Throop, Heather/0000-0002-7963-4342 FU John Wesley Powell Center for Analysis and Synthesis - USGS; US Environmental Protection Agency [EP-12-H-000491]; Cooperative Ecosystem Studies Units Network (National Park Service Grant) [P13AC00407]; Cooperative Ecosystem Studies Units Network (USGS Grant) [G14AC00028]; USGS FX Vegetation data were shared by the Forest Inventory and Analysis Database (FIADB) Vegetation Indicators Program, the Ecological Society of America VegBank, the Minnesota Biological Survey, the New York, Virginia, and West Virginia Natural Heritage Programs, Robert Peet and the Carolina Vegetation Survey, the US National Park Service Southern Colorado Plateau Network, the University of Wisconsin Plant Ecology Laboratory, Kevin Knutson of the US Geological Survey (USGS), and the coauthors. This paper arose from the "Diversity and Nitrogen Deposition" working group supported by the John Wesley Powell Center for Analysis and Synthesis, funded by the USGS. The US Environmental Protection Agency (Contract EP-12-H-000491) and the Cooperative Ecosystem Studies Units Network (National Park Service Grant P13AC00407 and USGS Grant G14AC00028) provided additional funding. The USGS supports the conclusions of research conducted by their employees, and peer reviews and approves all of their products consistent with USGS Fundamental Science Practices. The views expressed in this manuscript do not necessarily reflect the views or policies of the US Environmental Protection Agency or the USDA Forest Service. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 40 TC 8 Z9 8 U1 37 U2 69 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 12 PY 2016 VL 113 IS 15 BP 4086 EP 4091 DI 10.1073/pnas.1515241113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DI8PG UT WOS:000373762400055 PM 27035943 ER PT J AU Stiegel, MA Pleil, JD Sobus, JR Madden, MC AF Stiegel, Matthew A. Pleil, Joachim D. Sobus, Jon R. Madden, Michael C. TI Inflammatory Cytokines and White Blood Cell Counts Response to Environmental Levels of Diesel Exhaust and Ozone Inhalation Exposures SO PLOS ONE LA English DT Article ID AIR-POLLUTION; PARTICULATE MATTER; HEALTHY HUMANS; HUMAN EXPOSOME; NO2 EMISSIONS; LUNG-FUNCTION; BIOMARKERS; VOLUNTEERS; DISEASE; MICE AB Epidemiological observations of urban inhalation exposures to diesel exhaust (DE) and ozone (O-3) have shown pre-clinical cardiopulmonary responses in humans. Identifying the key biological mechanisms that initiate these health bioindicators is difficult due to variability in environmental exposure in time and from person to person. Previously, environmentally controlled human exposure chambers have been used to study DE and O-3 dose-response patterns separately, but investigation of co-exposures has not been performed under controlled conditions. Because a mixture is a more realistic exposure scenario for the general public, in this study we investigate the relationships of urban levels of urban-level DE exposure (300 mu g/m(3)), O-3 (0.3 ppm), DE + O-3 co-exposure, and innate immune system responses. Fifteen healthy human volunteers were studied for changes in ten inflammatory cytokines (interleukins 1 beta, 2, 4, 5, 8, 10, 12p70 and 13, IFN-gamma, and TNF-alpha) and counts of three white blood cell types (lymphocytes, monocytes, and neutrophils) following controlled exposures to DE, O3, and DE+O-3. The results show subtle cytokines responses to the diesel-only and ozone-only exposures, and that a more complex (possibly synergistic) relationship exists in the combination of these two exposures with suppression of IL-5, IL-12p70, IFN-gamma, and TNF-alpha that persists up to 22-hours for IFN-gamma and TNF-alpha. The white blood cell differential counts showed significant monocyte and lymphocyte decreases and neutrophil increases following the DE + O3 exposure; lymphocytes and neutrophils changes also persist for at least 22-hours. Because human studies must be conducted under strict safety protocols at environmental levels, these effects are subtle and are generally only seen with detailed statistical analysis. This study indicates that the observed associations between environmental exposures and cardiopulmonary effects are possibly mediated by inflammatory response mechanisms. C1 [Stiegel, Matthew A.] Duke Univ, Med Ctr, Dept Occupat & Environm Safety, Div Occupat Hyg & Safety, Durham, NC USA. [Pleil, Joachim D.; Sobus, Jon R.] US EPA, Natl Exposure Res Lab, Human Exposure & Atmospher Sci Div, Res Triangle Pk, NC 27711 USA. [Madden, Michael C.] US EPA, Natl Hlth & Environm Effects Res Lab, Environm Publ Hlth Div, Chapel Hill, NC USA. RP Pleil, JD (reprint author), US EPA, Natl Exposure Res Lab, Human Exposure & Atmospher Sci Div, Res Triangle Pk, NC 27711 USA. EM pleil.joachim@epa.gov FU US Government FX The authors JDP, JRS, MCM and the study were fully funded by the US Government as part of their employment. NR 58 TC 2 Z9 2 U1 2 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 8 PY 2016 VL 11 IS 4 AR e0152458 DI 10.1371/journal.pone.0152458 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DI6JK UT WOS:000373604800006 PM 27058360 ER PT J AU Stoddard, JL Van Sickle, J Herlihy, AT Brahney, J Paulsen, S Peck, DV Mitchell, R Pollard, AI AF Stoddard, John L. Van Sickle, John Herlihy, Alan T. Brahney, Janice Paulsen, Steven Peck, David V. Mitchell, Richard Pollard, Amina I. TI Continental-Scale Increase in Lake and Stream Phosphorus: Are Oligotrophic Systems Disappearing in the United States? SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID WIND RIVER RANGE; SURVEY DESIGN; FRESH-WATER; ECOSYSTEMS; DEPOSITION; RESOURCES; POLLUTION; NITROGEN; RECORD; INPUTS AB We describe continental-scale increases in lake and stream total phosphorus (TP) concentrations, identified through periodic probability surveys of thousands of water bodies in the conterminous U.S. The increases, observed over the period 2000-2014 were most notable in sites in relatively undisturbed catchments and where TP was initially low (e.g., less than 10 mu g L-1). Nationally, the percentage of stream length in the U.S. with TP <= 10 mu g L-1 decreased from 24.5 to 10.4 to 1.6% from 2004 to 2009 to 2014; the percentage of lakes with TP <= 10 mu g L-1 decreased from 24.9 to 6.7% between 2007 and 2012. Increasing TP concentrations appear to be ubiquitous, but their presence in undeveloped catchments suggests that they cannot be entirely attributed to either point or common non-point sources of TP. C1 [Stoddard, John L.; Van Sickle, John; Paulsen, Steven; Peck, David V.] US EPA, 200 SW 35Th St, Corvallis, OR 97333 USA. [Herlihy, Alan T.] Oregon State Univ, Dept Fish & Wildlife, Corvallis, OR 97331 USA. [Brahney, Janice] Univ British Columbia, Dept Earth & Environm Sci, Kelowna, BC V1V 1V7, Canada. [Mitchell, Richard; Pollard, Amina I.] US EPA, Off Water, Washington, DC 20460 USA. RP Stoddard, JL (reprint author), US EPA, 200 SW 35Th St, Corvallis, OR 97333 USA. EM stoddard.john@epa.gov OI Brahney, Janice/0000-0001-7614-2855 NR 33 TC 6 Z9 6 U1 17 U2 35 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD APR 5 PY 2016 VL 50 IS 7 BP 3409 EP 3415 DI 10.1021/acs.est.5b05950 PG 7 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DI7CD UT WOS:000373655800014 PM 26914108 ER PT J AU Hou, WC He, CJ Wang, YS Wang, DK Zepp, RG AF Hou, Wen-Che He, Chen-Jing Wang, Yi-Sheng Wang, David K. Zepp, Richard G. TI Phototransformation-Induced Aggregation of Functionalized Single-Walled Carbon Nanotubes: The Importance of Amorphous Carbon SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID DISSOLVED ORGANIC-MATTER; RADICAL RATE CONSTANTS; SURFACE FUNCTIONALIZATION; ENVIRONMENTAL FATE; HYDROXYL RADICALS; MOLECULAR-WEIGHT; UV-IRRADIATION; WATER; RELEASE; OXIDATION AB Single-walled carbon nanotubes (SWCNTs) with proper functionalization are desirable for applications that require dispersion in aqueous and biological environments, and functionalized SWCNTs, also serve as building blocks for conjugation with specific molecules in these applications. In this study, we examined the phototransformation of carboxylated SWCNTs and associated amorphous carbon impurities in the presence or absence of H2O2 under simulated sunlight conditions. We found that while carboxylated SWCNTs were rather unreactive with respect to direct solar photolysis, they photoreacted in the presence of H2O2, forming CO2 and strongly aggregated SWCNT products that precipitated. Photoreaction caused SWCNTs to lose oxygen-containing functionalities, and interestingly, the resulting photoproducts had spectral characteristics similar to those of parent carboxylated SWCNTs whose amorphous carbon was removed by base washing. These results indicated that photoreaction of the amorphous carbon was likely involved. The removal of amorphous carbon after indirect photoreaction was confirmed with thermogravimetric analysis (TGA). Further studies using carboxylated SWCNTs with and without base washing indicate that amorphous carbon reduced the extent of aggregation caused by photoreaction. The second-order rate constant for carboxylated SWCNTs reacting with *OH was estimated to be in the range of 1.7-3.8 X 10(9) M-C(-1) s(-1). The modeled phototransformation half-lives fall in the range of 2.8-280 days in typical sunlit freshwaters. Our study indicates that photosensitized reactions involving (OH)-O-center dot may be a transformation and removal pathway of functionalized SWCNTs in the aquatic environment, and that the residual amorphous carbon associated with SWCNTs plays a role in SWCNT stabilization. C1 [Hou, Wen-Che; He, Chen-Jing; Wang, Yi-Sheng] Natl Cheng Kung Univ, Dept Environm Engn, Tainan 70101, Taiwan. [Zepp, Richard G.] US EPA, Natl Exposure Res Lab, Exposure Methods & Measurement Div, Athens, GA 30605 USA. [Wang, David K.] Univ Queensland, Sch Chem Engn, FIMLab Films & Inorgan Membrane Lab, Brisbane, Qld 4072, Australia. RP Hou, WC (reprint author), Natl Cheng Kung Univ, Dept Environm Engn, Tainan 70101, Taiwan.; Zepp, RG (reprint author), US EPA, Natl Exposure Res Lab, Exposure Methods & Measurement Div, Athens, GA 30605 USA. EM whou@mail.ncku.edu.tw; zepp.richard@epa.gov RI Wang, David/A-9763-2011; Hou, Wen-Che/F-5736-2011 OI Wang, David/0000-0002-0294-7738; Hou, Wen-Che/0000-0001-9884-2932 FU Ministry of Science and Technology (MOST) of Taiwan [103-2221-E-006-015-MY3]; Ministry of Education, Taiwan, R.O.C.; Australian Research Council via the Discovery Early Career Researcher Award [DE150101687] FX Financial support provided by the Ministry of Science and Technology (MOST) of Taiwan (for Hou) under Grant 103-2221-E-006-015-MY3 is acknowledged. This paper has been reviewed in accordance with the U.S. Environmental Protection Agency's (U.S. EPA) peer and administrative review policies and approved for publication. Mention of trade names or commercial products does not constitute an endorsement or recommendation for use by the U.S. EPA. This research was, in part, supported by the Ministry of Education, Taiwan, R.O.C. The Aim for the Top University Project to the National Cheng Kung University (NCKU). D.K.W. is grateful for the support given by the Australian Research Council via the Discovery Early Career Researcher Award (DE150101687). NR 66 TC 1 Z9 1 U1 8 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD APR 5 PY 2016 VL 50 IS 7 BP 3494 EP 3502 DI 10.1021/acs.est.5b04727 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DI7CD UT WOS:000373655800024 PM 26928260 ER PT J AU Pleil, JD AF Pleil, Joachim D. TI Comparing biomarker measurements to a normal range: when to use standard error of the mean (SEM) or standard deviation (SD) confidence intervals tests SO BIOMARKERS LA English DT Editorial Material DE Confidence interval; sample size; standard error; statistics ID STATISTICS AB This commentary is the second of a series outlining one specific concept in interpreting biomarkers data. In the first, an observational method was presented for assessing the distribution of measurements before making parametric calculations. Here, the discussion revolves around the next step, the choice of using standard error of the mean or the calculated standard deviation to compare or predict measurement results C1 [Pleil, Joachim D.] US EPA, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Pleil, JD (reprint author), US EPA, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. EM pleil@email.unc.edu NR 10 TC 2 Z9 2 U1 5 U2 7 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1354-750X EI 1366-5804 J9 BIOMARKERS JI Biomarkers PD APR 2 PY 2016 VL 21 IS 3 BP 195 EP 199 DI 10.3109/1354750X.2015.1134666 PG 5 WC Biotechnology & Applied Microbiology; Toxicology SC Biotechnology & Applied Microbiology; Toxicology GA DI3IP UT WOS:000373392600001 PM 27045663 ER PT J AU Choudhary, R Klauda, JB AF Choudhary, Rehan Klauda, Jeffery B. TI The simultaneous mass and energy evaporation (SM2E) model SO JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL HYGIENE LA English DT Article DE risk assessment; mathematical modeling; Environmental modeling; Liquid mixtures; exposure assessment; evaporation rate ID VOLATILE ORGANIC-COMPOUNDS; LIQUID; SPILLS; RATES; PREDICTION; EXPOSURE AB In this article, the Simultaneous Mass and Energy Evaporation (SM2E) model is presented. The SM2E model is based on theoretical models for mass and energy transfer. The theoretical models systematically under or over predicted at various flow conditions: laminar, transition, and turbulent. These models were harmonized with experimental measurements to eliminate systematic under or over predictions; a total of 113 measured evaporation rates were used. The SM2E model can be used to estimate evaporation rates for pure liquids as well as liquid mixtures at laminar, transition, and turbulent flow conditions. However, due to limited availability of evaporation data, the model has so far only been tested against data for pure liquids and binary mixtures. The model can take evaporative cooling into account and when the temperature of the evaporating liquid or liquid mixture is known (e.g., isothermal evaporation), the SM2E model reduces to a mass transfer-only model. C1 [Choudhary, Rehan] US EPA, Off Pollut Prevent & Tox, Risk Assessment Div, Washington, DC 20460 USA. [Klauda, Jeffery B.] Univ Maryland, Dept Chem & Biomol Engn, 4418 Stadium Dr, College Pk, MD 20742 USA. RP Klauda, JB (reprint author), Univ Maryland, Dept Chem & Biomol Engn, 4418 Stadium Dr, College Pk, MD 20742 USA. EM jbklauda@umd.edu NR 23 TC 0 Z9 0 U1 1 U2 4 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1545-9624 EI 1545-9632 J9 J OCCUP ENVIRON HYG JI J. Occup. Environ. Hyg. PD APR 2 PY 2016 VL 13 IS 4 BP 247 EP 257 DI 10.1080/15459624.2015.1101123 PG 11 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DF9TN UT WOS:000371704200001 PM 26595699 ER PT J AU Morris, JT Barber, DC Callaway, JC Chambers, R Hagen, SC Hopkinson, CS Johnson, BJ Megonigal, P Neubauer, SC Troxler, T Wigand, C AF Morris, James T. Barber, Donald C. Callaway, John C. Chambers, Randy Hagen, Scott C. Hopkinson, Charles S. Johnson, Beverly J. Megonigal, Patrick Neubauer, Scott C. Troxler, Tiffany Wigand, Cathleen TI Contributions of organic and inorganic matter to sediment volume and accretion in tidal wetlands at steady state SO EARTHS FUTURE LA English DT Article ID SEA-LEVEL RISE; MARSH VERTICAL ACCRETION; MISSISSIPPI RIVER DELTA; BULK-DENSITY; SPARTINA-ALTERNIFLORA; SALT-MARSHES; FRESH-WATER; FOREST SOILS; DECOMPOSITION; BAY AB A mixing model derived from first principles describes the bulk density ( BD) of intertidal wetland sediments as a function of loss on ignition (LOI). The model assumes that the bulk volume of sediment equates to the sum of self-packing volumes of organic and mineral components or BD = 1/[LOI/k(1) + (1-LOI)/k(2)], where k(1) and k(2) are the self-packing densities of the pure organic and inorganic components, respectively. The model explained 78% of the variability in total BD when fitted to 5075 measurements drawn from 33 wetlands distributed around the conterminous United States. The values of k(1) and k(2) were estimated to be 0.085 +/- 0.0007 g cm(-3) and 1.99 +/- 0.028 g cm(-3), respectively. Based on the fitted organic density (k(1)) and constrained by primary production, the model suggests that the maximum steady state accretion arising from the sequestration of refractory organic matter is <= 0.3 cm yr(-1). Thus, tidal peatlands are unlikely to indefinitely survive a higher rate of sea-level rise in the absence of a significant source of mineral sediment. Application of k(2) to a mineral sediment load typical of East and eastern Gulf Coast estuaries gives a vertical accretion rate from inorganic sediment of 0.2 cm yr(-1). Total steady state accretion is the sum of the parts and therefore should not be greater than 0.5 cm yr(-1) under the assumptions of the model. Accretion rates could deviate from this value depending on variation in plant productivity, root: shoot ratio, suspended sediment concentration, sediment-capture efficiency, and episodic events. C1 [Morris, James T.] Univ South Carolina, Dept Biol Sci, Belle W Baruch Inst Marine & Coastal Sci, Columbia, SC 29208 USA. [Barber, Donald C.] Bryn Mawr Coll, Dept Geol, Bryn Mawr, PA 19010 USA. [Callaway, John C.] Univ Calif San Francisco, Dept Environm Sci, San Francisco, CA 94143 USA. [Chambers, Randy] Coll William & Mary, Keck Environm Field Lab, Williamsburg, VA USA. [Hagen, Scott C.] Louisiana State Univ, Dept Civil & Environm Engn, Baton Rouge, LA 70803 USA. [Hagen, Scott C.] Louisiana State Univ, Ctr Computat & Technol, Baton Rouge, LA 70803 USA. [Hopkinson, Charles S.] Univ Georgia, Dept Marine Sci, Athens, GA 30602 USA. [Johnson, Beverly J.] Bates Coll, Dept Geol, Lewiston, ME 04240 USA. [Megonigal, Patrick] Smithsonian Environm Res Ctr, POB 28, Edgewater, MD 21037 USA. [Neubauer, Scott C.] Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. [Troxler, Tiffany] Florida Int Univ, Dept Biol Sci, Southeast Environm Res Ctr, Miami, FL 33199 USA. [Wigand, Cathleen] US EPA, Atlantic Ecol Div, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Narragansett, RI USA. RP Morris, JT (reprint author), Univ South Carolina, Dept Biol Sci, Belle W Baruch Inst Marine & Coastal Sci, Columbia, SC 29208 USA. EM morris@biol.sc.edu FU NOAA [NA10NOS4780146]; NSF DEB [1052636, 0620409, 9910514, 1237517, 1127958, 0950090, 1457100, 1355059]; NSF EAR [1322859, 1322658]; NSF [OCE-1238212]; NICCR [DE-FC02-06ER64298]; NASA [NNH14AY67]; Bates College Student Research Fund; Bryn Mawr College Summer Science Research Program; Conservation International; IUCN; IOC-UNESCO FX This research was supported by NOAA grant NA10NOS4780146, NSF DEB grants 1052636, 0620409, 9910514, 1237517, 1127958, 0950090, 1457100 and 1355059, NSF EAR grants 1322859 and 1322658, NSF OCE-1238212, NICCR grant DE-FC02-06ER64298, NASA grant NNH14AY67, the Bates College Student Research Fund, the Bryn Mawr College Summer Science Research Program, and the Blue Carbon Initiative coordinated by Conservation International, IUCN, and IOC-UNESCO. NR 89 TC 5 Z9 5 U1 25 U2 38 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2328-4277 J9 EARTHS FUTURE JI Earth Future PD APR PY 2016 VL 4 IS 4 BP 110 EP 121 DI 10.1002/2015EF000334 PG 12 WC Environmental Sciences; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Geology; Meteorology & Atmospheric Sciences GA DR1MD UT WOS:000379668700002 PM 27819012 ER PT J AU Bullerjahn, GS Mckay, RM Davis, TW Baker, DB Boyer, GL D'Anglada, LV Doucette, GJ Ho, JC Irwin, EG Kling, CL Kudela, RM Kurmayer, R Michalak, AM Ortiz, JD Otten, TG Paerl, HW Qin, BQ Sohngen, BL Stumpf, RP Visser, PM Wilhelm, SW AF Bullerjahn, George S. McKay, Robert M. Davis, Timothy W. Baker, David B. Boyer, Gregory L. D'Anglada, Lesley V. Doucette, Gregory J. Ho, Jeff C. Irwin, Elena G. Kling, Catherine L. Kudela, Raphael M. Kurmayer, Rainer Michalak, Anna M. Ortiz, Joseph D. Otten, Timothy G. Paerl, Hans W. Qin, Boqiang Sohngen, Brent L. Stumpf, Richard P. Visser, Petra M. Wilhelm, Steven W. TI Global solutions to regional problems: Collecting global expertise to address the problem of harmful cyanobacterial blooms. A Lake Erie case study SO HARMFUL ALGAE LA English DT Review DE Cyanobacteria; CHAB; Lake Erie; Microcystin; Phosphorus; Nitrogen ID CO2 CONCENTRATING MECHANISMS; WATER-QUALITY; TOXIC CYANOBACTERIA; ALGAL BLOOMS; AGRICULTURAL PHOSPHORUS; RE-EUTROPHICATION; CLIMATE-CHANGE; SHALLOW LAKES; MAUMEE RIVER; GREAT-LAKES AB In early August 2014, the municipality of Toledo, OH (USA) issued a 'do not drink' advisory on their water supply directly affecting over 400,000 residential customers and hundreds of businesses (Wilson, 2014). This order was attributable to levels of microcystin, a potent liver toxin, which rose to 2.5 mu g L-1 in finished drinking water. The Toledo crisis afforded an opportunity to bring together scientists from around the world to share ideas regarding factors that contribute to bloom formation and toxigenicity, bloom and toxin detection as well as prevention and remediation of bloom events. These discussions took place at an NSF- and NOAA-sponsored workshop at Bowling Green State University on April 13 and 14, 2015. In all, more than 100 attendees from six countries and 15 US states gathered together to share their perspectives. The purpose of this review is to present the consensus summary of these issues that emerged from discussions at the Workshop. As additional reports in this special issue provide detailed reviews on many major CHAB species, this paper focuses on the general themes common to all blooms, such as bloom detection, modeling, nutrient loading, and strategies to reduce nutrients. (C) 2016 Elsevier B.V. All rights reserved. C1 [Bullerjahn, George S.; McKay, Robert M.] Bowling Green State Univ, Dept Biol Sci, Life Sci Bldg, Bowling Green, OH 43403 USA. [Davis, Timothy W.] NOAA, GLERL, 4840 South State Rd, Ann Arbor, MI 48108 USA. [Baker, David B.] Heidelberg Univ, Natl Ctr Water Qual Res, Tiffin, OH 44883 USA. [Boyer, Gregory L.] SUNY Coll Environm Sci & Forestry, Dept Chem, 1 Forestry Dr, Syracuse, NY 13210 USA. [D'Anglada, Lesley V.] US EPA, Agcy Off Sci & Technol, 1200 Penn Ave NW, Washington, DC 20460 USA. [Doucette, Gregory J.] NOAA, Natl Ocean Serv, 219 Ft Johnson Rd, Charleston, SC 29412 USA. [Ho, Jeff C.] Stanford Univ, Dept Civil & Environm Engn, 473 Via Ortega, Stanford, CA 94305 USA. [Ho, Jeff C.; Michalak, Anna M.] Carnegie Inst Sci, Dept Global Ecol, 260 Panama St, Stanford, CA 94305 USA. [Irwin, Elena G.; Sohngen, Brent L.] Ohio State Univ, Dept Agr Environm & Dev Econ, 2120 Fyffe Rd, Columbus, OH 43210 USA. [Kling, Catherine L.] Iowa State Univ Sci & Technol, Dept Econ, 578 Heady Hall, Ames, IA 50011 USA. [Kudela, Raphael M.] Univ Calif Santa Cruz, Ocean Sci, 1156 High St, Santa Cruz, CA 95064 USA. [Kurmayer, Rainer] Univ Innsbruck, Res Inst Limnol, Mondseestr 9, A-5310 Mondsee, Austria. [Ortiz, Joseph D.] Kent State Univ, Dept Geol, 336 McGilvrey Hall, Kent, OH 44242 USA. [Otten, Timothy G.] Oregon State Univ, Dept Microbiol, 226 Nash Hall, Corvallis, OR 97331 USA. [Paerl, Hans W.] Univ N Carolina, Inst Marine Sci, 3431 Arendell St, Morehead City, NC 28557 USA. [Qin, Boqiang] Nanjing Inst Geog & Limnol, 73 East Beijing Rd, Nanjing 210008, Peoples R China. [Stumpf, Richard P.] NOAA, Natl Ctr Coastal Ocean Sci, 1305 East West Highway Code N SCI1, Silver Spring, MD 20910 USA. [Visser, Petra M.] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Dept Aquat Microbiol, POB 94248, NL-1090 GE Amsterdam, Netherlands. [Wilhelm, Steven W.] Univ Tennessee, Dept Microbiol, 1414 West Cumberland Ave, Knoxville, TN 37996 USA. RP Bullerjahn, GS (reprint author), Bowling Green State Univ, Dept Biol Sci, Life Sci Bldg, Bowling Green, OH 43403 USA. EM bullerj@bgsu.edu RI Wilhelm, Steven/B-8963-2008 OI Wilhelm, Steven/0000-0001-6283-8077 FU National Science Foundation [CBET-1515671]; NOAA Great Lakes Environmental Research Laboratory; University of Michigan Water Center; Office of the Vice President for Research AMP; Economic Development at Bowling Green State University; Ohio Department of Education [R/HAB-2-BOR] FX The CHAB Workshop was supported by the National Science Foundation under grant no. CBET-1515671 (GSB). Additional support was provided by the NOAA Great Lakes Environmental Research Laboratory, the University of Michigan Water Center and by the Office of the Vice President for Research & Economic Development at Bowling Green State University. Additional research support was provided by Ohio Department of Education grant R/HAB-2-BOR to GSB, RMM and JDO. GSB, RMM and SWW thank Mr. Jason Isakovic (Senior Legislative Assistant, office of US Rep. Robert Latta) for helpful discussions on the legislative process. The authors also thank the many staff and students at BGSU who provided assistance prior and during the Workshop.[SS] NR 148 TC 7 Z9 7 U1 38 U2 70 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1568-9883 EI 1878-1470 J9 HARMFUL ALGAE JI Harmful Algae PD APR PY 2016 VL 54 SI SI BP 223 EP 238 DI 10.1016/j.hal.2016.01.003 PG 16 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA DN8HK UT WOS:000377319600016 PM 28073479 ER PT J AU McClelland, KS Ng, ET Bowles, J AF McClelland, Kathryn S. Ee Ting Ng Bowles, Josephine TI Agarose/gelatin immobilisation of tissues or embryo segments for orientated paraffin embedding and sectioning SO DIFFERENTIATION LA English DT Review DE Embedding; Sectioning; Orientated sample; Agarose; Immobilisation AB The technique described in this protocol allows the user to position small tissues in the optimal orientation for paraffin embedding and sectioning by first immobilising the tissue in an agarose/gelatin cube. This method is an adaptation of methods used for early embryos and can be used for any small tissues or embryo segments. Processing of larger tissue sections using molds to create agarose/gelatin blocks has been described previously; this detailed protocol provides a method for dealing with much smaller tissues or embryos (<= 5 mm). The tissue is briefly fixed then an agarose/gelatin drop is created to surround the tissue. The tissue can be orientated as per the user's preference in the drop before it sets as is carved into a cube with a domed top. The cube is then dehydrated and goes through the embedding and sectioning process. The domed cube is easy to orientate when embedding the tissue in a wax block giving the user assured orientation of the small tissue for sectioning. Additionally, the agarose/gelatin cube is easy to see in the unmolded wax once embedded, making the region of interest easy to identify. Crown Copyright (C) 2016 Published by Elsevier B.V. on behalf of International Society of Differentiation All rights reserved. C1 [McClelland, Kathryn S.; Ee Ting Ng; Bowles, Josephine] Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia. [McClelland, Kathryn S.] Natl Inst Environm Hlth Sci, Reprod Dev Biol Lab, Dev Reprod Biol Grp, Res Triangle Pk, NC USA. RP Bowles, J (reprint author), Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia. EM j.bowles@imb.uq.edu.au RI Bowles, Josephine/B-6583-2013; OI Bowles, Josephine/0000-0003-2867-7438; McClelland, Kathryn S/0000-0003-2845-5448 FU Australian Research Council (ARC); National Health and Medical Research Council of Australia (NHMRC) FX We thank Terje Svingen and Peter Koopman for supervising the work that refined this protocol. We thank Ivy Chiang for helpful discussions. This work was supported by research grants from the Australian Research Council (ARC) and the National Health and Medical Research Council of Australia (NHMRC). Confocal microscopy was performed at the Australian Cancer Research Foundation Dynamic Imaging Centre for Cancer Biology. NR 5 TC 0 Z9 0 U1 2 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0301-4681 EI 1432-0436 J9 DIFFERENTIATION JI Differentiation PD APR-JUN PY 2016 VL 91 IS 4-5 BP 68 EP 71 DI 10.1016/j.diff.2015.12.001 PG 4 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DO2OQ UT WOS:000377619800010 PM 26742717 ER PT J AU Arini, A Cavallin, JE Berninger, JP Marfil-Vega, R Mills, M Villeneuve, DL Basu, N AF Arini, Adeline Cavallin, Jenna E. Berninger, Jason P. Marfil-Vega, Ruth Mills, Marc Villeneuve, Daniel L. Basu, Niladri TI In vivo and In vitro neurochemical-based assessments of wastewater effluents from the Maumee River area of concern SO ENVIRONMENTAL POLLUTION LA English DT Article DE Wastewater; Neurochemistry; In vitro cell-free assay; Dopamine; GABA ID MONOAMINE-OXIDASE ACTIVITY; SEWAGE-TREATMENT PLANT; NEUROENDOCRINE DISRUPTION; ELLIPTIO-COMPLANATA; FISH; RECEPTORS; INDUCTION; MERCURY; STREAM; PHARMACEUTICALS AB Wastewater treatment plant (WWTP) effluents contain potentially neuroactive chemicals though few methods are available to screen for the presence of such agents. Here, two parallel approaches (in vivo and in vitro) were used to assess WWTP exposure-related changes to neurochemistry. First, fathead minnows (FHM, Pimephales promelas) were caged for four days along a WWTP discharge zone into the Maumee River (Ohio, USA). Grab water samples were collected and extracts obtained for the detection of alkylphenols, bisphenol A (BPA) and steroid hormones. Second, the extracts were then used as a source of in vitro exposure to brain tissues from FHM and four additional species relevant to the Great Lakes ecosystem (rainbow trout (RT), river otter (RO), bald eagle (BE) and human (HU)). The ability of the wastewater (in vivo) or extracts (in vitro) to interact with enzymes (monoamine oxidase (MAO) and glutamine synthetase (GS)) and receptors (dopamine (D2) and N-methyl-D-aspartate receptor (NMDA)) involved in dopamine and glutamate-dependent neurotransmission were examined on brain homogenates. In vivo exposure of FHM led to significant decreases of NMDA receptor binding in females (24 -42%), and increases of MAO activity in males (2.8- to 3.2-fold). In vitro, alkylphenol-targeted extracts significantly inhibited D2 (66% in FHM) and NMDA (24-54% in HU and RT) receptor binding, and induced MAO activity in RT, RO, and BE brains. Steroid hormone-targeted extracts inhibited GS activity in all species except FHM. BPA-targeted extracts caused a MAO inhibition in FHM, RT and BE brains. Using both in vivo and in vitro approaches, this study shows that WWTP effluents contain agents that can interact with neurochemicals important in reproduction and other neurological functions. Additional work is needed to better resolve in vitro to in vivo extrapolations (IVIVE) as well as cross-species differences. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Arini, Adeline; Basu, Niladri] Univ Michigan, Dept Environm Hlth Sci, Ann Arbor, MI 48109 USA. [Arini, Adeline; Basu, Niladri] McGill Univ, Fac Agr & Environm Sci, Montreal, PQ, Canada. [Cavallin, Jenna E.] US EPA, ORISE Res Participat Program, Midcontinent Ecol Div, Duluth, MN USA. [Berninger, Jason P.] US Geol Survey, Columbia Environm Res Ctr, Columbia, MO USA. [Marfil-Vega, Ruth] Amer Water Innovat & Environm Stewardship, Belleville, IL USA. [Mills, Marc] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Villeneuve, Daniel L.] US EPA, Midcontinent Ecol Div, Duluth, MN USA. RP Basu, N (reprint author), 21,111 Lakeshore, Ste Anne De Bellevue, PQ H9X 3V9, Canada. EM nialdri.basu@mcgill.ca RI Berninger, Jason/O-2401-2016; Mills, Marc/C-3449-2017; OI Berninger, Jason/0000-0003-3045-7899; Mills, Marc/0000-0002-0169-3086; Basu, Niladri/0000-0002-2695-1037 FU U.S. EPA Science to Achieve Results (STAR) Program [R835170] FX This research was partly supported by funding from the U.S. EPA Science to Achieve Results (STAR) Program to NB (grant number R835170). Additional support for the caged fish exposures, sample collection, and logistics were provided by Evan P. Eid, Kyle E. Stevens, Megan N. Hughes, Michael D. Kahl, Kathleen M. Jensen, JoAnn Banda. We thank Dr. Johan F. Gottgens, University of Toledo for providing lab space for the fish necropsy. Thanks to Scott Weasel, Christine Harmon, Chris Middlebrough at the Toledo Bay View Wastewater Treatment Plant. The contents of this paper have been reviewed by the US EPA Office of Research and Development. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. The contents neither constitute, nor necessarily reflect, official US EPA policy. NR 41 TC 0 Z9 0 U1 13 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD APR PY 2016 VL 211 BP 9 EP 19 DI 10.1016/j.envpol.2015.12.028 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DM9OY UT WOS:000376696800002 PM 26736051 ER PT J AU Willming, MM Maul, JD AF Willming, Morgan M. Maul, Jonathan D. TI Direct and indirect toxicity of the fungicide pyraclostrobin to Hyalella azteca and effects on leaf processing under realistic daily temperature regimes SO ENVIRONMENTAL POLLUTION LA English DT Article DE Fungicides; Diurnal temperature; Organic matter decomposition; Climate change ID TARGETED USE AREAS; LITTER BREAKDOWN; UNITED-STATES; STREAM FUNGI; WATER; INVERTEBRATES; FORMULATIONS; AMPHIPOD; TADPOLES AB Fungicides in aquatic environments can impact non-target bacterial and fungal communities and the invertebrate detritivores responsible for the decomposition of allochthonous organic matter. Additionally, in some aquatic systems daily water temperature fluctuations may influence these processes and alter contaminant toxicity, but such temperature fluctuations are rarely examined in conjunction with contaminants. In this study, the shredding amphipod Hyalella azteca was exposed to the fungicide pyraclostrobin in three experiments. Endpoints included mortality, organism growth, and leaf processing. One experiment was conducted at a constant temperature (23 degrees C), a fluctuating temperature regime (18-25 degrees C) based on field-collected data from the S. Llano River, Texas, or an adjusted fluctuating temperature regime (20-26 degrees C) based on possible climate change predictions. Pyraclostrobin significantly reduced leaf shredding and increased H. azteca mortality at concentrations of 40 mu g/L or greater at a constant 23 degrees C and decreased leaf shredding at concentrations of 15 mu g/L or greater in the fluctuating temperatures. There was a significant interaction between temperature treatment and pyraclostrobin concentration on H. azteca mortality, body length, and dry mass under direct aqueous exposure conditions. In an indirect exposure scenario in which only leaf material was exposed to pyraclostrobin, H. azteca did not preferentially feed on or avoid treated leaf disks compared to controls. This study describes the influence of realistic temperature variation on fungicide toxicity to shredding invertebrates, which is important for understanding how future alterations in daily temperature regimes due to climate change may influence the assessment of ecological risk of contaminants in aquatic ecosystems. (C) 2016 Published by Elsevier Ltd. C1 [Willming, Morgan M.; Maul, Jonathan D.] Texas Tech Univ, Dept Environm Toxicol, Box 41163, Lubbock, TX 79409 USA. [Willming, Morgan M.] US EPA, Natl Hlth & Environm Effects Lab, Gulf Ecol Div, 1 Sabine Isl Dr, Gulf Breeze, FL 32561 USA. RP Maul, JD (reprint author), Texas Tech Univ, Dept Environm Toxicol, Box 41163, Lubbock, TX 79409 USA. EM jmaul5@gmail.com FU Achievement Rewards for College Scientists Foundation FX We would like to thank Tom Arsuffi for use of the Texas Tech Llano River Field Station, Donn Edwards for assistance in the field and laboratory, Maria Nunez for laboratory assistance, and Todd Anderson for help with chemical analyses. The Achievement Rewards for College Scientists Foundation scholarship provided support for M. Willming. NR 37 TC 0 Z9 0 U1 5 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD APR PY 2016 VL 211 BP 435 EP 442 DI 10.1016/j.envpol.2015.11.029 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DM9OY UT WOS:000376696800049 PM 26827148 ER PT J AU Knightes, CD Golden, HE Journey, CA Davis, GM Conrads, PA Marvin-DiPasquale, M Brigham, ME Bradley, PM AF Knightes, Christopher D. Golden, Heather E. Journey, Celeste A. Davis, Gary M. Conrads, Paul A. Marvin-DiPasquale, Mark Brigham, Mark E. Bradley, Paul M. TI Mercury and methylmercury stream concentrations in a Coastal Plain watershed: A multi-scale simulation analysis (vol 187, pg 182, 2014) SO ENVIRONMENTAL POLLUTION LA English DT Correction C1 [Knightes, Christopher D.; Davis, Gary M.] US EPA, Athens, GA 30324 USA. [Golden, Heather E.] US EPA, Cincinnati, OH 45268 USA. [Journey, Celeste A.; Conrads, Paul A.; Bradley, Paul M.] US Geol Survey, Columbia, SC USA. [Marvin-DiPasquale, Mark] US Geol Survey, 345 Middlefield Rd, Menlo Pk, CA 94025 USA. [Brigham, Mark E.] US Geol Survey, Mounds View, MN USA. RP Knightes, CD (reprint author), US EPA, Athens, GA 30324 USA. NR 1 TC 0 Z9 0 U1 3 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD APR PY 2016 VL 211 BP 443 EP 443 DI 10.1016/j.envpol.2015.10.022 PG 1 WC Environmental Sciences SC Environmental Sciences & Ecology GA DM9OY UT WOS:000376696800050 ER PT J AU Glaser, JA AF Glaser, John A. TI Synthetic biology leading to specialty chemicals SO CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY LA English DT News Item C1 [Glaser, John A.] US EPA, Natl Risk Management Res Lab, 26 W King Dr, Cincinnati, OH 45268 USA. RP Glaser, JA (reprint author), US EPA, Natl Risk Management Res Lab, 26 W King Dr, Cincinnati, OH 45268 USA. EM Glaser.John@epa.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1618-954X EI 1618-9558 J9 CLEAN TECHNOL ENVIR JI Clean Technol. Environ. Policy PD APR PY 2016 VL 18 IS 4 BP 983 EP 989 DI 10.1007/s10098-016-1177-0 PG 7 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA DM0EO UT WOS:000376015900002 ER PT J AU Sundaravadivelu, D Suidan, MT Venosa, AD Rosales, PI Campo-Moreno, P Conmy, RN AF Sundaravadivelu, Devi Suidan, Makram T. Venosa, Albert D. Rosales, Pablo I. Campo-Moreno, Pablo Conmy, Robyn N. TI Development of a testing protocol for oil solidifier effectiveness evaluation SO CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY LA English DT Article DE Oil spill; Oil solidifier; Crude Oil; Salinity; Protocol; Effectiveness ID SPILL-TREATING AGENTS AB Chemical countermeasures for oil spill remediation have to be evaluated and approved by the U.S. Environmental Protection Agency before they may be used to remove or control oil discharges. Solidifiers are chemical agents that change oil from a liquid to a solid by immobilizing the oil and bonding the liquid into a solid carpet-like mass with minimal volume increase. Currently, they are listed as Miscellaneous Oil Spill Control Agent in the National Contingency Plan and there is no protocol for evaluating their effectiveness. An investigation was conducted to test the oil removal efficiency of solidifiers using three newly developed testing protocols. The protocols were qualitatively and quantitatively evaluated to determine if they can satisfactorily differentiate effective and mediocre products while still accounting for experimental error. The repeatability of the three protocols was 15.9, 5.1, and 2.7 %. The protocol with the best performance involved measuring the amount of free oil remaining in the water after the solidified product was removed using an ultraviolet-visible spectrophotometer and it was adopted to study the effect of solidifier-to-oil mass ratio, mixing energy, salinity, and beaker size (i.e., area affected by the spill) on solidifier efficiency. Analysis of Variances were performed on the data collected and results indicated that the beaker size increased spreading, which reduced removal efficiency. Mixing speed appears to impart a ceiling effect with no additional benefit provided by the highest level over the middle level. Salinity was found to be mostly an insignificant factor on performance. C1 [Sundaravadivelu, Devi] Univ Cincinnati, Dept Biomed Chem & Environm Engn, Cincinnati, OH 45221 USA. [Suidan, Makram T.] Amer Univ Beirut, Fac Engn & Architecture, Beirut 11072020, Lebanon. [Venosa, Albert D.; Conmy, Robyn N.] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Rosales, Pablo I.] Univ Cincinnati, Adv Mat Characterizat Ctr, Cincinnati, OH 45221 USA. [Campo-Moreno, Pablo] Cranfield Univ, Cranfield Water Sci Inst, Cranfield MK 43OAL, Beds, England. RP Suidan, MT (reprint author), Amer Univ Beirut, Fac Engn & Architecture, Beirut 11072020, Lebanon. EM msuidan@aub.edu.lb OI Campo, Pablo/0000-0001-8569-9620 FU U.S. EPA's National Risk Management Research Laboratory, Cincinnati, OH [EP-C-11-006] FX This research was supported in part by the U.S. EPA's National Risk Management Research Laboratory, Cincinnati, OH under Contract No. EP-C-11-006. The use of trade names or commercial products is for identification only and does not imply endorsement by any agency of the U.S. Government. NR 20 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1618-954X EI 1618-9558 J9 CLEAN TECHNOL ENVIR JI Clean Technol. Environ. Policy PD APR PY 2016 VL 18 IS 4 BP 1141 EP 1150 DI 10.1007/s10098-016-1107-1 PG 10 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA DM0EO UT WOS:000376015900014 ER PT J AU Fann, N Wesson, K Hubbell, B AF Fann, Neal Wesson, Karen Hubbell, Bryan TI Characterizing the confluence of air pollution risks in the United States SO AIR QUALITY ATMOSPHERE AND HEALTH LA English DT Article DE Multi-pollutant; PM2.5; Ozone; NAAQS; HAP ID QUALITY MANAGEMENT; HEALTH; OZONE AB The US National Research Council in 2004 recommended that the US Environmental Protection Agency "strive to take an integrated multipollutant approach to controlling emissions of pollutants that pose the most significant risks." Several urban pilot studies have since explored the merits and feasibility of this technique. These studies found that such policies can achieve this policy goal but will confront both legal constraints and data limitations. The Clean Air Act prescribes certain requirements for air quality polices reducing common (or "criteria") air pollutants including ground-level ozone and fine particles that differ from those affecting toxic air pollutants such as benzene; due in part to these requirements, policy makers have traditionally designed programs to characterize and mitigate risk on a pollutant-by-pollutant basis. Multipollutant policies are also greatly constrained by the emissions, air quality, and health data available to identify opportunities for reducing population risks across air pollutants. We introduce a novel approach to identify the confluence of particulate matter 2.5 mu m and smaller (PM2.5), ozone, and toxic air pollutant risk throughout the USA. We account for the federal statutes governing air pollution policy targeting these pollutants and address differences in the health data used to characterize the risks of these pollutants. We demonstrate a proof-of-concept technique for identifying areas of the USA where there may be a confluence of these risks. We argue that planners could use this approach as a resource as they achieve the goals identified by the National Research Council in 2004. C1 [Fann, Neal; Wesson, Karen; Hubbell, Bryan] US EPA, Off Air Qual Planning & Stand, 109 TW Alexander Dr, Durham, NC 27711 USA. RP Fann, N (reprint author), US EPA, Off Air Qual Planning & Stand, 109 TW Alexander Dr, Durham, NC 27711 USA. EM fann.neal@epa.gov OI Hubbell, Bryan/0000-0002-7963-3438 NR 30 TC 0 Z9 0 U1 3 U2 5 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 1873-9318 EI 1873-9326 J9 AIR QUAL ATMOS HLTH JI Air Qual. Atmos. Health PD APR PY 2016 VL 9 IS 3 BP 293 EP 301 DI 10.1007/s11869-015-0340-9 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK1IE UT WOS:000374664900009 ER PT J AU McCabe, JG Burke, TA AF McCabe, Janet G. Burke, Thomas A. TI REDUCING RISK BY ACTING ON CLIMATE SO HEALTH SECURITY LA English DT Editorial Material C1 [McCabe, Janet G.] US EPA, Off Air & Radiat, Washington, DC 20460 USA. [Burke, Thomas A.] US EPA, Off Res & Dev, 1200 Penn Ave NW,Mail Code 8101R, Washington, DC 20460 USA. RP Burke, TA (reprint author), US EPA, Off Res & Dev, 1200 Penn Ave NW,Mail Code 8101R, Washington, DC 20460 USA. EM burke.thomas@epa.gov NR 12 TC 1 Z9 1 U1 0 U2 0 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2326-5094 EI 2326-5108 J9 HEALTH SECUR JI Health Secur. PD APR PY 2016 VL 14 IS 2 BP 43 EP 46 DI 10.1089/hs.2016.0006 PG 4 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DK1FJ UT WOS:000374657600003 PM 27081882 ER PT J AU McCormick, S Madrigano, J Zinsmeister, E AF McCormick, Sabrina Madrigano, Jaime Zinsmeister, Emma TI PREPARING FOR EXTREME HEAT EVENTS: PRACTICES IN IDENTIFYING MORTALITY SO HEALTH SECURITY LA English DT Article ID AMBIENT-TEMPERATURE; MEDICAL EXAMINERS; CLIMATE-CHANGE; WAVE AB Climate change is increasing the frequency and severity of extreme heat events. These events affect cities in increasingly abrupt and catastrophic ways; yet, many of the deaths caused by exposure to heat have gone unnoticed or are inaccurately identified, resulting in a lack of urgency in addressing this issue. We aim to address this under-identification of deaths from heat waves in order to better assess heat risk. We investigated death records in New York City from 2010 to 2012 to identify characteristics that vary between deaths officially categorized as caused by heat wave exposure (oHDs) and those possibly caused by heat (pHDs). We found that oHDs were more often black and of a younger age than would typically be expected. We also found that there was a lack of evidence to substantiate that an oHD had occurred, using the NYC official criteria. We conclude that deaths from heat waves are not being accurately recorded, leading to a mis-estimation. Training regarding the collection and interpretation of evidence may improve preparedness for heat events. C1 [McCormick, Sabrina] George Washington Univ, Dept Environm & Occupat Hlth, Milken Inst Sch Publ Hlth, 950 New Hampshire Ave NW, Washington, DC 20052 USA. [Madrigano, Jaime] RAND Corp, Arlington, VA USA. [Zinsmeister, Emma] US EPA, Washington, DC 20460 USA. RP McCormick, S (reprint author), George Washington Univ, Dept Environm & Occupat Hlth, Milken Inst Sch Publ Hlth, 950 New Hampshire Ave NW, Washington, DC 20052 USA. EM sabmc@gwu.edu NR 31 TC 0 Z9 0 U1 0 U2 2 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2326-5094 EI 2326-5108 J9 HEALTH SECUR JI Health Secur. PD APR PY 2016 VL 14 IS 2 BP 55 EP 63 DI 10.1089/hs.2015.0048 PG 9 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DK1FJ UT WOS:000374657600006 PM 27081884 ER PT J AU McGinty, MD Burke, TA Resnick, BA Smith, KC Barnett, DJ Rutkow, L AF McGinty, Meghan D. Burke, Thomas A. Resnick, Beth A. Smith, Katherine C. Barnett, Daniel J. Rutkow, Lainie TI LEGAL PREPAREDNESS FOR HURRICANE SANDY: AUTHORITY TO ORDER HOSPITAL EVACUATION OR SHELTER-IN-PLACE IN THE MID-ATLANTIC REGION SO HEALTH SECURITY LA English DT Article AB Hospitals were once thought to be places of refuge during catastrophic hurricanes, but recent disasters such as Hurricanes Katrina and Sandy have demonstrated that some hospitals are unable to ensure the safety of patients and staff and the continuity of medical care at key times. The government has a duty to safeguard public health and a responsibility to ensure that appropriate protective action is taken when disasters threaten or impair the ability of hospitals to sustain essential services. The law can enable the government to fulfill this duty by providing necessary authority to order preventive or reactive responsessuch as ordering evacuation of or sheltering-in-place in hospitalswhen safety is imperiled. We systematically identified and analyzed state emergency preparedness laws that could have affected evacuation of and sheltering-in-place in hospitals in order to characterize the public health legal preparedness of 4 states (Delaware, Maryland, New Jersey, and New York) in the mid-Atlantic region during Hurricane Sandy in 2012. At that time, none of these 4 states had enacted statutes or regulations explicitly granting the government the authority to order hospitals to shelter-in-place. Whereas all 4 states had enacted laws explicitly enabling the government to order evacuation, the nature of this authority and the individuals empowered to execute it varied. We present empirical analyses intended to enhance public health legal preparedness and ensure these states and others are better able to respond to future natural disasters, which are predicted to be more severe and frequent as a result of climate change, as well as other hazards. States can further improve their readiness for catastrophic disasters by ensuring explicit statutory authority to order evacuation and to order sheltering-in-place, particularly of hospitals, where it does not currently exist. C1 [McGinty, Meghan D.] UPMC, Ctr Hlth Secur, 621 East Pratt St,Ste 210, Baltimore, MD 21202 USA. [McGinty, Meghan D.; Burke, Thomas A.; Resnick, Beth A.; Barnett, Daniel J.; Rutkow, Lainie] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Hlth Policy & Management, Baltimore, MD USA. [Burke, Thomas A.] US EPA, Off Res & Dev, Washington, DC 20460 USA. [Smith, Katherine C.] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Hlth Behav & Soc, Baltimore, MD USA. RP McGinty, MD (reprint author), UPMC, Ctr Hlth Secur, 621 East Pratt St,Ste 210, Baltimore, MD 21202 USA. EM mcgintymd@upmc.edu FU NIOSH Education and Research Center for Occupational Safety and Health at the Johns Hopkins Bloomberg School of Public Health [T42-OH 008428]; Johns Hopkins Environment, Energy, Sustainability & Health Institute (E2SHI) Fellowship; Lipitz Public Health Policy Award FX MDM was supported in part by funding from the NIOSH Education and Research Center for Occupational Safety and Health at the Johns Hopkins Bloomberg School of Public Health (#T42-OH 008428), the Johns Hopkins Environment, Energy, Sustainability & Health Institute (E2SHI) Fellowship, and the 2013-2014 Lipitz Public Health Policy Award. The funders had no role in the design and conduct of the study, or collection, management, analysis, and interpretation of the data. The contents of this article are solely the responsibility of the authors and do not necessarily represent the official views of any of the funders. NR 27 TC 1 Z9 1 U1 0 U2 2 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2326-5094 EI 2326-5108 J9 HEALTH SECUR JI Health Secur. PD APR PY 2016 VL 14 IS 2 BP 78 EP 85 DI 10.1089/hs.2015.0068 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DK1FJ UT WOS:000374657600009 PM 27081887 ER PT J AU Kleinstreuer, NC Sullivan, K Allen, D Edwards, S Mendrick, DL Embry, M Matheson, J Rowlands, JC Munn, S Maull, E Casey, W AF Kleinstreuer, Nicole C. Sullivan, Kristie Allen, David Edwards, Stephen Mendrick, Donna L. Embry, Michelle Matheson, Joanna Rowlands, J. Craig Munn, Sharon Maull, Elizabeth Casey, Warren TI Adverse outcome pathways: From research to regulation scientific workshop report SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Article DE AOP; Workshop; Toxicology; Pathway; Regulatory; Quantitative; Application; Acceptance; Criteria; Computational ID DILUTION MASS-SPECTROMETRY; EARLY FISH DEVELOPMENT; THYROPEROXIDASE INHIBITION; CONCEPTUAL-FRAMEWORK; VASCULAR DEVELOPMENT; TESTING STRATEGY; DECISION-MAKING; IPCS FRAMEWORK; MODE; CONFIDENCE AB An adverse outcome pathway (AOP) helps to organize existing knowledge on chemical mode of action, starting with a molecular initiating event such as receptor binding, continuing through key events, and ending with an adverse outcome such as reproductive impairment. AOPs can help identify knowledge gaps where more research is needed to understand the underlying mechanisms, aid in chemical hazard characterization, and guide the development of new testing approaches that use fewer or no animals. A September 2014 workshop in Bethesda, Maryland considered how the AOP concept could improve regulatory assessments of chemical toxicity. Scientists from 21 countries, representing industry, academia, regulatory agencies, and special interest groups, attended the workshop, titled Adverse Outcome Pathways: From Research to Regulation. Workshop plenary presentations were followed by breakout sessions that considered regulatory acceptance of AOPs and AOP-based tools, criteria for building confidence in an AOP for regulatory use, and requirements to build quantitative AOPs and AOP networks. Discussions during the closing session emphasized a need to increase transparent and inclusive collaboration, especially with disciplines outside of toxicology. Additionally, to increase impact, working groups should be established to systematically prioritize and develop AOPs. Multiple collaborative projects and follow-up activities resulted from the workshop. (C) 2016 Elsevier Inc. All rights reserved. C1 [Kleinstreuer, Nicole C.; Maull, Elizabeth; Casey, Warren] NIEHS, Natl Toxicol Program, Interagency Ctr Evaluat Alternat Toxicol Methods, POB 12233, Res Triangle Pk, NC 27709 USA. [Sullivan, Kristie] Phys Comm Responsible Med, Washington, DC USA. [Allen, David] Integrated Lab Syst Inc, Res Triangle Pk, NC USA. [Edwards, Stephen] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Mendrick, Donna L.] US FDA, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA. [Embry, Michelle] ILSI Hlth & Environm Sci Inst, Washington, DC USA. [Matheson, Joanna] US Consumer Prod Safety Commiss, Rockville, MD USA. [Rowlands, J. Craig] Dow Chem Co USA, Midland, MI 48674 USA. [Munn, Sharon] Commiss European Communities, Joint Res Ctr, I-21020 Ispra, Italy. RP Kleinstreuer, NC (reprint author), POB 13501, Res Triangle Pk, NC 27709 USA. EM Nicole.kleinstreuer@nih.gov OI Kleinstreuer, Nicole/0000-0002-7914-3682 FU federal funds from the NIEHS, NIH [HHSN273201400003C, HHSN273201500010C]; NICEATM FX The authors would like to thank all of the workshop presenters and breakout group moderators, Hristo Aladjov for assisting in creation of Fig. 3, George Daston for his contributions to the workshop steering committee discussions, Catherine Sprankle for editorial comments, NICEATM staff Michael Paris, Steven Morefield, Neepa Choksi, and Catherine Sprankle for assistance with organizing and running the workshop, and PCRM staff Arya Birdie and Mallory Huff for their assistance with organizing the workshop. Nicole Kleinstreuer and David Allen were supported by federal funds from the NIEHS, NIH under Contract Numbers HHSN273201400003C and HHSN273201500010C to Integrated Laboratory Systems, Inc., in support of NICEATM. The views represented in this paper are those of the authors solely, and do not represent the official policy of the U.S. EPA, FDA, NIH, or any other federal government organization. NR 45 TC 6 Z9 6 U1 6 U2 13 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0273-2300 EI 1096-0295 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD APR PY 2016 VL 76 BP 39 EP 50 DI 10.1016/j.yrtph.2016.01.007 PG 12 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA DL3DK UT WOS:000375514200005 PM 26774756 ER PT J AU Poet, TS Schlosser, PM Rodriguez, CE Parod, RJ Rodwell, DE Kirman, CR AF Poet, T. S. Schlosser, P. M. Rodriguez, C. E. Parod, R. J. Rodwell, D. E. Kirman, C. R. TI Using physiologically based pharmacokinetic modeling and benchmark dose methods to derive an occupational exposure limit for N-methylpyrrolidone SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Article DE Occupational exposure limit; Benchmark dose; PBPK modeling; Developmental toxicity; Window of susceptibility ID SPRAGUE-DAWLEY RATS; ADVERSE EFFECT LEVELS; DEVELOPMENTAL TOXICITY; N-METHYL-2-PYRROLIDONE NMP; PERCUTANEOUS-ABSORPTION; INHALATION EXPOSURE; RESPONSE ASSESSMENT; DERMAL ABSORPTION; METABOLISM; PHTHALATE AB The developmental effects of NMP are well studied in Sprague Dawley rats following oral, inhalation, and dermal routes of exposure. Short-term and chronic occupational exposure limit (OEL) values were derived using an updated physiologically based pharmacokinetic (PBPK) model for NMP, along with benchmark dose modeling. Two suitable developmental endpoints were evaluated for human health risk assessment: (1) for acute exposures, the increased incidence of skeletal malformations, an effect noted only at oral doses that were toxic to the dam and fetus; and (2) for repeated exposures to NMP, changes in fetal/pup body weight. Where possible, data from multiple studies were pooled to increase the predictive power of the dose response data sets. For the purposes of internal dose estimation, the window of susceptibility was estimated for each endpoint, and was used in the dose response modeling. A point of departure value of 390 mg/L (in terms of peak NMP in blood) was calculated for skeletal malformations based on pooled data from oral and inhalation studies. Acceptable dose response model fits were not obtained using the pooled data for fetal/pup body weight changes. These data sets were also assessed individually, from which the geometric mean value obtained from the inhalation studies (470 mg*hr/L), was used to derive the chronic OEL. A PBPK model for NMP in humans was used to calculate human equivalent concentrations corresponding to the internal dose point of departure values. Application of a net uncertainty factor of 20-21, which incorporates data-derived extrapolation factors, to the point of departure values yields short-term and chronic occupational exposure limit values of 86 and 24 ppm, respectively. (C) 2016 The Authors. Published by Elsevier Inc. C1 [Poet, T. S.] Summit Toxicol, Richland, WA USA. [Schlosser, P. M.] US EPA, Res Triangle Pk, NC 27711 USA. [Rodriguez, C. E.] US EPA, Washington, DC 20460 USA. [Rodriguez, C. E.] Coca Cola Co, Atlanta, GA USA. [Parod, R. J.] BASF Corp, Wyandotte, MI USA. [Rodwell, D. E.] Rodwell & Associates, Houston, TX USA. [Kirman, C. R.] Summit Toxicol, Orange Village, OH USA. RP Kirman, CR (reprint author), Summit Toxicol, Orange Village, OH USA. EM ckirman@summittoxicology.com FU NMP Producers Groups FX Co-authors TSP, DER, and CRK are independent consultants who have received funding from the NMP Producers Groups for their work on this manuscript. The funders of this work were given an opportunity to review a draft manuscript for the purposes of improving its clarity, but did not have a role in methods design, data collection and analysis, or results interpretation. NR 49 TC 1 Z9 1 U1 5 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0273-2300 EI 1096-0295 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD APR PY 2016 VL 76 BP 102 EP 112 DI 10.1016/j.yrtph.2015.12.020 PG 11 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA DL3DK UT WOS:000375514200013 PM 26776754 ER PT J AU Angrish, MM Kaiser, JP McQueen, CA Chorley, BN AF Angrish, Michelle M. Kaiser, Jonathan Phillip McQueen, Charlene A. Chorley, Brian N. TI Tipping the Balance: Hepatotoxicity and the 4 Apical Key Events of Hepatic Steatosis SO TOXICOLOGICAL SCIENCES LA English DT Review DE non-alcoholic fatty liver disease; steatosis; adverse outcome pathway; mechanistic toxicology; high-throughput screening assays; chemical risk assessment ID STEAROYL-COA DESATURASE-1; NONALCOHOLIC FATTY LIVER; TOXICANT-ASSOCIATED STEATOHEPATITIS; ELEMENT-BINDING PROTEIN-1C; LOW-DENSITY LIPOPROTEIN; X-RECEPTOR LXR; ACID-METABOLISM; RISK-ASSESSMENT; NUTRITIONAL REGULATION; MEDIATED INDUCTION AB Hepatic steatosis is a condition were fat accumulates in the liver and it is associated with extra-hepatic diseases related to metabolic syndrome and systemic energy metabolism. If not reversed, steatosis can progress to steatohepatitis and irreversible stages of liver disease including fibrosis, cirrhosis, hepatocellular carcinoma, and death. From a public health standpoint, identifying chemical exposures that may be factors in steatosis etiology are important for preventing hepatotoxicity and liver disease progression. It is therefore important to identify the biological events that are key for steatosis pathology mediated by chemical exposure. In this review, we give a current overview of the complex biological cascades that can disrupt lipid homeostasis in hepatocytes in the context of 4 apical key events central to hepatic lipid retention: hepatic fatty acid (FA) uptake, de novo FA and lipid synthesis, FA oxidation, and lipid efflux. Our goal is to review these key cellular events and visually summarize them using a network for application in pathway-based toxicity testing. This effort provides a foundation to improve next-generation chemical screening efforts that may be used to prevent and ultimately reverse the growing incidence of fatty liver disease in our population. C1 [Angrish, Michelle M.; McQueen, Charlene A.; Chorley, Brian N.] US EPA, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27709 USA. [Kaiser, Jonathan Phillip] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Cincinnati, OH 45268 USA. RP Chorley, BN (reprint author), Natl Hlth & Environm Effects Res Lab, 109 TW Alexander Dr,Mail Drop B-105-03, Res Triangle Pk, NC 27711 USA. EM chorley.brian@epa.gov FU U.S. Environmental Protection Agency Office of Research and Development FX Internal funding for this study came from the U.S. Environmental Protection Agency Office of Research and Development. NR 61 TC 2 Z9 2 U1 2 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD APR PY 2016 VL 150 IS 2 BP 261 EP 268 DI 10.1093/toxsci/kfw018 PG 8 WC Toxicology SC Toxicology GA DJ5ET UT WOS:000374230300002 PM 26980302 ER PT J AU Miller, DB Snow, SJ Schladweiler, MC Richards, JE Ghio, AJ Ledbetter, AD Kodavanti, UP AF Miller, Desinia B. Snow, Samantha J. Schladweiler, Mette C. Richards, Judy E. Ghio, Andrew J. Ledbetter, Allen D. Kodavanti, Urmila P. TI Acute Ozone-Induced Pulmonary and Systemic Metabolic Effects Are Diminished in Adrenalectomized Rats SO TOXICOLOGICAL SCIENCES LA English DT Article DE ozone; adrenalectomy; stress response; HPA-axis; lung injury ID INHALED PARTICULATE MATTER; BROWN-NORWAY RATS; AIR-POLLUTION; INSULIN-RESISTANCE; GENE-EXPRESSION; VAGAL AFFERENTS; GLUCOSE-METABOLISM; DIABETES-MELLITUS; HEART-RATE; EXPOSURE AB Acute ozone exposure increases circulating stress hormones and induces metabolic alterations in animals. We hypothesized that the increase of adrenal-derived stress hormones is necessary for both ozone-induced metabolic effects and lung injury. Male Wistar-Kyoto rats underwent bilateral adrenal demedullation (DEMED), total bilateral adrenalectomy (ADREX), or sham surgery (SHAM). After a 4 day recovery, rats were exposed to air or ozone (1 ppm), 4 h/day for 1 or 2 days and responses assessed immediately postexposure. Circulating adrenaline levels dropped to nearly zero in DEMED and ADREX rats relative to SHAM. Corticosterone tended to be low in DEMED rats and dropped to nearly zero in ADREX rats. Adrenalectomy in air-exposed rats caused modest changes in metabolites and lung toxicity parameters. Ozone-induced hyperglycemia and glucose intolerance were markedly attenuated in DEMED rats with nearly complete reversal in ADREX rats. Ozone increased circulating epinephrine and corticosterone in SHAM but not in DEMED or ADREX rats. Free fatty acids (P = .15) and branched-chain amino acids increased after ozone exposure in SHAM but not in DEMED or ADREX rats. Lung minute volume was not affected by surgery or ozone but ozone-induced labored breathing was less pronounced in ADREX rats. Ozone-induced increases in lung protein leakage and neutrophilic inflammation were markedly reduced in DEMED and ADREX rats (ADREX > DEMED). Ozone-mediated decreases in circulating white blood cells in SHAM were not observed in DEMED and ADREX rats. We demonstrate that ozone-induced peripheral metabolic effects and lung injury/inflammation are mediated through adrenal-derived stress hormones likely via the activation of stress response pathway. C1 [Miller, Desinia B.] Univ N Carolina, Curriculum Toxicol, Chapel Hill, NC 27599 USA. [Snow, Samantha J.; Schladweiler, Mette C.; Richards, Judy E.; Ghio, Andrew J.; Ledbetter, Allen D.; Kodavanti, Urmila P.] US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. RP Kodavanti, UP (reprint author), US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. EM kodavanti.urmila@epa.gov OI Snow, Samantha/0000-0003-1812-8582 FU United States Environmental Protection Agency funds; United States Environmental Protection Agency [CR835152010]; University of North Carolina Toxicology [CR835152010] FX This work was supported by the United States Environmental Protection Agency funds, and the Co-Operative Agreement between United States Environmental Protection Agency and University of North Carolina Toxicology Training Agreement CR835152010 to D.B.M. NR 63 TC 3 Z9 3 U1 2 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD APR PY 2016 VL 150 IS 2 BP 312 EP 322 DI 10.1093/toxsci/kfv331 PG 11 WC Toxicology SC Toxicology GA DJ5ET UT WOS:000374230300007 PM 26732886 ER PT J AU Karmaus, AL Toole, CM Filer, DL Lewis, KC Martin, MT AF Karmaus, Agnes L. Toole, Colleen M. Filer, Dayne L. Lewis, Kenneth C. Martin, Matthew T. TI High-Throughput Screening of Chemical Effects on Steroidogenesis Using H295R Human Adrenocortical Carcinoma Cells SO TOXICOLOGICAL SCIENCES LA English DT Article DE steroidogenesis; H295R cells; ToxCast; high-throughput screening ID ENDOCRINE-DISRUPTING CHEMICALS; MURINE LEYDIG-CELLS; ESTROGEN-RECEPTOR; STEROID-HORMONES; IN-VITRO; MODEL; LINE; PATHWAY; BIOSYNTHESIS; DYSGENESIS AB Disruption of steroidogenesis by environmental chemicals can result in altered hormone levels causing adverse reproductive and developmental effects. A high-throughput assay using H295R human adrenocortical carcinoma cells was used to evaluate the effect of 2060 chemical samples on steroidogenesis via high-performance liquid chromatography followed by tandem mass spectrometry quantification of 10 steroid hormones, including progestagens, glucocorticoids, androgens, and estrogens. The study employed a 3 stage screening strategy. The first stage established the maximum tolerated concentration (MTC; a parts per thousand yen 70% viability) per sample. The second stage quantified changes in hormone levels at the MTC whereas the third stage performed concentration-response (CR) on a subset of samples. At all stages, cells were prestimulated with 10 A mu M forskolin for 48 h to induce steroidogenesis followed by chemical treatment for 48 h. Of the 2060 chemical samples evaluated, 524 samples were selected for 6-point CR screening, based in part on significantly altering at least 4 hormones at the MTC. CR screening identified 232 chemical samples with concentration-dependent effects on 17 beta-estradiol and/or testosterone, with 411 chemical samples showing an effect on at least one hormone across the steroidogenesis pathway. Clustering of the concentration-dependent chemical-mediated steroid hormone effects grouped chemical samples into 5 distinct profiles generally representing putative mechanisms of action, including CYP17A1 and HSD3B inhibition. A distinct pattern was observed between imidazole and triazole fungicides suggesting potentially distinct mechanisms of action. From a chemical testing and prioritization perspective, this assay platform provides a robust model for high-throughput screening of chemicals for effects on steroidogenesis. C1 [Karmaus, Agnes L.; Filer, Dayne L.; Martin, Matthew T.] US EPA, Natl Ctr Computat Toxicol, 109 TW Alexander Dr,Mail Drop D143-02, Res Triangle Pk, NC 27711 USA. [Toole, Colleen M.] CeeTox Inc, Cyprotex, Kalamazoo, MI USA. [Lewis, Kenneth C.] OpAns LLC, Durham, NC USA. RP Martin, MT (reprint author), US EPA, Natl Ctr Computat Toxicol, 109 TW Alexander Dr,Mail Drop D143-02, Res Triangle Pk, NC 27711 USA. EM martin.matt@epa.gov RI Karmaus, Agnes/L-2147-2015 OI Karmaus, Agnes/0000-0003-4421-6164 NR 35 TC 2 Z9 2 U1 7 U2 13 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD APR PY 2016 VL 150 IS 2 BP 323 EP 332 DI 10.1093/toxsci/kfw002 PG 10 WC Toxicology SC Toxicology GA DJ5ET UT WOS:000374230300008 PM 26781511 ER PT J AU Bell, SM Angrish, MM Wood, CE Edwards, SW AF Bell, Shannon M. Angrish, Michelle M. Wood, Charles E. Edwards, Stephen W. TI Integrating Publicly Available Data to Generate Computationally Predicted Adverse Outcome Pathways for Fatty Liver SO TOXICOLOGICAL SCIENCES LA English DT Article DE computationally predicted adverse outcome pathways; cpAOP; TG-GATEs; network integration; fatty liver; steatosis ID CARBON-TETRACHLORIDE; ONTOLOGY; DISEASE; BIOLOGY; IDENTIFICATION; BIOCONDUCTOR; STRATEGIES; FRAMEWORK; CHEMICALS; OBESITY AB New in vitro testing strategies make it possible to design testing batteries for large numbers of environmental chemicals. Full utilization of the results requires knowledge of the underlying biological networks and the adverse outcome pathways (AOPs) that describe the route from early molecular perturbations to an adverse outcome. Curation of a formal AOP is a time-intensive process and a rate-limiting step to designing these test batteries. Here, we describe a method for integrating publicly available data in order to generate computationally predicted AOP (cpAOP) scaffolds, which can be leveraged by domain experts to shorten the time for formal AOP development. A network-based workflow was used to facilitate the integration of multiple data types to generate cpAOPs. Edges between graph entities were identified through direct experimental or literature information, or computationally inferred using frequent itemset mining. Data from the TG-GATEs and ToxCast programs were used to channel large-scale toxicogenomics information into a cpAOP network (cpAOPnet) of over 20 000 relationships describing connections between chemical treatments, phenotypes, and perturbed pathways as measured by differential gene expression and high-throughput screening targets. The resulting fatty liver cpAOPnet is available as a resource to the community. Subnetworks of cpAOPs for a reference chemical (carbon tetrachloride, CCl4) and outcome (fatty liver) were compared with published mechanistic descriptions. In both cases, the computational approaches approximated the manually curated AOPs. The cpAOPnet can be used for accelerating expert-curated AOP development and to identify pathway targets that lack genomic markers or high-throughput screening tests. It can also facilitate identification of key events for designing test batteries and for classification and grouping of chemicals for follow up testing. C1 [Bell, Shannon M.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Bell, Shannon M.; Angrish, Michelle M.; Wood, Charles E.; Edwards, Stephen W.] US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Bell, Shannon M.] NTP Interagcy Ctr Evaluat Alternat Toxicol Method, Res Triangle Pk, NC USA. RP Edwards, SW (reprint author), US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, 109 TW Alexander Dr,Mail Code B105-03, Res Triangle Pk, NC 27709 USA. EM edwards.stephen@epa.gov FU U. S. Environmental Protection Agency FX This work was supported by the U. S. Environmental Protection Agency. S.M.B. was supported by an appointment to the Internship/Research Participation Program at the Office of Research and Development, U.S. EPA, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. EPA. NR 33 TC 5 Z9 5 U1 4 U2 16 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD APR PY 2016 VL 150 IS 2 BP 510 EP 520 DI 10.1093/toxsci/kfw017 PG 11 WC Toxicology SC Toxicology GA DJ5ET UT WOS:000374230300022 PM 26895641 ER PT J AU Fu, X Cheng, Z Wang, SX Hua, Y Xing, J Hao, JM AF Fu, Xiao Cheng, Zhen Wang, Shuxiao Hua, Yang Xing, Jia Hao, Jiming TI Local and Regional Contributions to Fine Particle Pollution in Winter of the Yangtze River Delta, China SO AEROSOL AND AIR QUALITY RESEARCH LA English DT Article DE Fine particle pollution; YRD; Local accumulation; Regional contribution ID SURFACE MODELING TECHNIQUE; PARTICULATE MATTER; NONLINEAR RESPONSE; HAZE POLLUTION; SOURCE APPORTIONMENT; SENSITIVITY-ANALYSIS; AIR-QUALITY; IMPACT; EMISSIONS; AEROSOLS AB The Yangtze River Delta (YRD) is one of the most developed and heavily polluted regions in China. Understanding the origin of fine particles is necessary for policy making on air pollution control in this region. However, the current studies on quantifying the contributions from different emission source regions are limited, especially for winter pollution. In this study, field observations and scenario analysis simulations were performed by using the Weather Research and Forecasting and Community Multi-scale Air Quality (WRF/CMAQ) model to quantify the impacts of local emissions and long-range regional transport in a winter period of 2011. The results indicated that the local contributions were dominant on average in the whole period, especially for slight or medium pollution hours. However, when particle pollution increased, the effects of long-range regional transport significantly increased. The inner-regional effects from Jiangsu and the outer regional effects from the upwind adjacent province, Shandong, are crucial for the YRD region. The dominant contributor could be local accumulation or long-range regional transport, which usually depends on the weather conditions. This study provides a systematic analysis of the characteristics and origins of winter pollution in the YRD according to combined observations and modeling, which is helpful for understanding regional particle pollution formation and policy making on improving air quality in this region. C1 [Fu, Xiao; Wang, Shuxiao; Hua, Yang; Hao, Jiming] Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China. [Cheng, Zhen] Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200240, Peoples R China. [Wang, Shuxiao; Hao, Jiming] State Environm Protect Key Lab Sources & Control, Beijing 100084, Peoples R China. [Xing, Jia] US EPA, Res Triangle Pk, NC 27711 USA. RP Wang, SX (reprint author), Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China.; Wang, SX (reprint author), State Environm Protect Key Lab Sources & Control, Beijing 100084, Peoples R China. EM shxwang@tsinghua.edu.cn RI wang, shuxiao/H-5990-2011 OI wang, shuxiao/0000-0001-9727-1963 FU National Natural Science Foundation of China [21221004]; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB05020300] FX This work was sponsored by National Natural Science Foundation of China (21221004) and Strategic Priority Research Program of the Chinese Academy of Sciences (XDB05020300). The authors also appreciate the support from Collaborative Innovation Center for Regional Environmental Quality of Tsinghua University. Our work was completed on the Explorer 100 cluster system of Tsinghua National Laboratory for Information Science and Technology. NR 43 TC 0 Z9 0 U1 7 U2 16 PU TAIWAN ASSOC AEROSOL RES-TAAR PI TAICHUNG COUNTY PA CHAOYANG UNIV TECH, DEPT ENV ENG & MGMT, PROD CTR AAQR, NO 168, JIFONG E RD, WUFONG TOWNSHIP, TAICHUNG COUNTY, 41349, TAIWAN SN 1680-8584 EI 2071-1409 J9 AEROSOL AIR QUAL RES JI Aerosol Air Qual. Res. PD APR PY 2016 VL 16 IS 4 BP 1067 EP 1080 DI 10.4209/aaqr.2015.08.0496 PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK2UV UT WOS:000374770800013 ER PT J AU Watson, EB Szura, K Wigand, C Raposa, KB Blount, K Cencer, M AF Watson, E. B. Szura, K. Wigand, C. Raposa, K. B. Blount, K. Cencer, M. TI Sea level rise, drought and the decline of Spartina patens in New England marshes SO BIOLOGICAL CONSERVATION LA English DT Article DE Climate change; Marsh die-back; Storms; Precipitation; Spartina alterniflora; Marsh organ; Thin layer deposition; TLD; Saltmarsh sparrow ID VESICULAR-ARBUSCULAR MYCORRHIZAE; SHARP-TAILED SPARROWS; RIVER DELTAIC PLAIN; SALT-MARSH; VEGETATION CHANGE; HYDROGEN SULFIDE; SALINITY STRESS; SEASIDE SPARROW; UNITED-STATES; CAPE-COD AB Already heavily impacted by coastal development, estuarine vegetated habitats (seagrasses, salt marshes, and mangroves) are increasingly affected by climate change via accelerated sea level rise, changes in the frequency and intensity of precipitation and storms, and warmer ocean temperatures. Conservation of these sensitive and vulnerable habitats requires the recognition of climate change effects so environmental managers can develop and apply appropriate intervention and adaptation strategies where possible. Here we focus on Spartina patens (saltmeadow cordgrass), a foundation species of New England (USA) coastal marshes. A growing, body of evidence suggests this species is disappearing rapidly from wetlands in the region, leading to reductions in habitat quality, plant diversity, carbon sequestration, erosion resistance and coastal protection. We grew S. patens under five inundation and three precipitation regimes, monitored changes in its extent within two Southern New England coastal marshes (2000-2014), and used water level and precipitation data to detect changes in environmental conditions affecting these marshes. Our results suggest that current inundation patterns have reduced the persistence of S. patens, while short-term drought did not appear responsible for vegetation changes or habitat conversion. We conclude that accelerated sea level rise is incompatible with the long-term survival of S. patens within the current landscape footprint of Southern New England's coastal wetlands. We suggest that conservation actions focused on high marsh preservation concentrate on facilitating the process of marsh migration onto uplands by prioritizing buffer conservation, conducting barrier removal and allowing for retreat where feasible. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Watson, E. B.] Drexel Univ, Acad Nat Sci, Biodivers Earth & Environm Sci Dept, Philadelphia, PA 19104 USA. [Szura, K.; Wigand, C.] US EPA, Atlantic Ecol Div, ORD NHEERL, Narragansett, RI USA. [Raposa, K. B.] Narragansett Bay Natl Estuarine Res Reserve, Prudence Isl, RI USA. [Blount, K.] Univ Oregon, Dept Environm Studies, Eugene, OR 97403 USA. [Cencer, M.] Michigan Technol Univ, Dept Chem, Houghton, MI 49931 USA. RP Watson, EB (reprint author), Drexel Univ, Acad Nat Sci, Patrick Ctr Environm Res, 1900 Benjamin Franklin Pkwy, Philadelphia, PA 19103 USA. EM elizabeth.b.watson@gmail.com FU EPA GRO fellowships [91752201-1, 91754401-0]; U.S. Environmental Protection Agency FX We acknowledge K. Miller for assistance with statistical analyses, K. Kelly for assistance in maintaining the flow-through seawater system, J. Bishop, D. Vendettuoli, and S. Kelly for field and laboratory assistance, and W. Ferguson, M. Cole Ekberg, and S. Paton for helpful discussions. This work was funded in part by EPA GRO fellowships awarded to K. Blount (91752201-1) and M. Cencer (91754401-0). Although the information in this document has in part been funded by the U.S. Environmental Protection Agency, it does not necessarily reflect the views of the Agency and no official endorsement should be inferred. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 72 TC 2 Z9 2 U1 42 U2 65 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD APR PY 2016 VL 196 BP 173 EP 181 DI 10.1016/j.biocon.2016.02.011 PG 9 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DL1AW UT WOS:000375365300020 ER PT J AU Rappazzo, KM Warren, JL Meyer, RE Herring, AH Sanders, AP Brownstein, NC Luben, TJ AF Rappazzo, Kristen M. Warren, Joshua L. Meyer, Robert E. Herring, Amy H. Sanders, Alison P. Brownstein, Naomi C. Luben, Thomas J. TI Maternal residential exposure to agricultural pesticides and birth defects in a 2003 to 2005 North Carolina birth cohort SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Article DE pesticide exposure; residential; agriculture; birth defects; congenital anomalies; GIS ID SAN-JOAQUIN VALLEY; NEURAL-TUBE DEFECTS; ATRAZINE EXPOSURE; UNITED-STATES; RISK; CALIFORNIA; HYPOSPADIAS; PROXIMITY; PREGNANCY; CHILDREN AB BackgroundBirth defects are responsible for a large proportion of disability and infant mortality. Exposure to a variety of pesticides have been linked to increased risk of birth defects. MethodsWe conducted a case-control study to estimate the associations between a residence-based metric of agricultural pesticide exposure and birth defects. We linked singleton live birth records for 2003 to 2005 from the North Carolina (NC) State Center for Health Statistics to data from the NC Birth Defects Monitoring Program. Included women had residence at delivery inside NC and infants with gestational ages from 20 to 44 weeks (n=304,906). Pesticide exposure was assigned using a previously constructed metric, estimating total chemical exposure (pounds of active ingredient) based on crops within 500 meters of maternal residence, specific dates of pregnancy, and chemical application dates based on the planting/harvesting dates of each crop. Logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals for four categories of exposure (<10(th), 10-50(th), 50-90(th), and >90(th) percentiles) compared with unexposed. Models were adjusted for maternal race, age at delivery, education, marital status, and smoking status. ResultsWe observed elevated ORs for congenital heart defects and certain structural defects affecting the gastrointestinal, genitourinary and musculoskeletal systems (e.g., OR [95% confidence interval] [highest exposure vs. unexposed] for tracheal esophageal fistula/esophageal atresia=1.98 [0.69, 5.66], and OR for atrial septal defects: 1.70 [1.34, 2.14]). ConclusionOur results provide some evidence of associations between residential exposure to agricultural pesticides and several birth defects phenotypes. Birth Defects Research (Part A) 106:240-249, 2016. (c) 2016 Wiley Periodicals, Inc. C1 [Rappazzo, Kristen M.] US EPA, Natl Ctr Environm Assessment, Oak Ridge Inst Sci & Educ, Res Triangle Pk, NC 27711 USA. [Warren, Joshua L.] Yale Univ, Sch Publ Hlth, Dept Biostat, New Haven, CT USA. [Meyer, Robert E.] North Carolina Dept Hlth & Human Serv, Raleigh, NC USA. [Herring, Amy H.] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Biostat, Chapel Hill, NC USA. [Sanders, Alison P.] Icahn Sch Med Mt Sinai, Dept Prevent Med, New York, NY 10029 USA. [Brownstein, Naomi C.] Florida State Univ, Coll Med, Dept Behav Sci & Social Med, Tallahassee, FL 32306 USA. [Brownstein, Naomi C.] Florida State Univ, Dept Stat, Tallahassee, FL 32306 USA. [Luben, Thomas J.] US EPA, Natl Ctr Environm Assessment, Res Triangle Pk, NC 27711 USA. RP Rappazzo, KM (reprint author), US EPA, Natl Ctr Environm Assessment, Oak Ridge Inst Sci & Educ, Res Triangle Pk, NC 27711 USA. EM rappazzo.kristen@epa.gov OI Brownstein, Naomi/0000-0002-9991-427X; Sanders, Alison/0000-0001-8252-4016 FU U.S. Department of Energy; EPA; National Institute of Environmental Health Sciences [T32ES007018, P30ES010126, R01ES020619]; National Birth Defect Prevention Study CDC funds; NSF [0646083] FX Supported in part by an appointment to the Internship/Research Participation Program at Office of Research and Development (National Center for Environmental Assessment), U.S. Environmental Protection Agency, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. This research was supported in part by grants from the National Institute of Environmental Health Sciences (T32ES007018, P30ES010126, R01ES020619), National Birth Defect Prevention Study CDC funds, and the NSF Graduate Research Fellowship Program grant 0646083. NR 35 TC 1 Z9 1 U1 3 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD APR PY 2016 VL 106 IS 4 BP 240 EP 249 DI 10.1002/bdra.23479 PG 10 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA DJ7GN UT WOS:000374380000003 PM 26970546 ER PT J AU Patlewicz, G Fitzpatrick, JM AF Patlewicz, Grace Fitzpatrick, Jeremy M. TI Current and Future Perspectives on the Development, Evaluation, and Application of in Silico Approaches for Predicting Toxicity SO CHEMICAL RESEARCH IN TOXICOLOGY LA English DT Review ID DERMAL SENSITIZATION THRESHOLD; INDUCED LIVER-INJURY; VIVO TOXICOLOGICAL OUTCOMES; STRUCTURAL INCLUSION RULES; THROUGHPUT SCREENING DATA; DECISION-SUPPORT-SYSTEM; READ-ACROSS; APPLICABILITY DOMAIN; QSAR MODELS; COMPUTATIONAL PREDICTION AB Exploiting non-testing approaches to predict toxicity early in the drug-discovery development cycle is a helpful component in minimizing expensive drug failures due to toxicity being identified in late development or even during clinical trials. Changes in regulations in the industrial chemicals and cosmetics sectors in recent years have prompted a significant number of advances in the development; application, and assessment of non-testing approaches,, such as (Q)SARs. Many efforts have also been undertaken to establish guiding principles for performing read-across within category and analogue approaches. This review offers a perspective, as taken from these sectors, of the current status of non-testing approaches, their evolution in light of the advances in high-throughput approaches and constructs such as adverse outcome pathways, and their potential relevance for drug discovery. It also proposes a workflow for how non-testing approaches could be practically integrated within testing and assessment strategies. C1 [Patlewicz, Grace; Fitzpatrick, Jeremy M.] US EPA, NCCT, Res Triangle Pk, NC 27711 USA. RP Patlewicz, G (reprint author), US EPA, NCCT, Res Triangle Pk, NC 27711 USA. EM gpatlewicz@gmail.com OI Patlewicz, Grace/0000-0003-3863-9689 NR 93 TC 7 Z9 7 U1 8 U2 19 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0893-228X EI 1520-5010 J9 CHEM RES TOXICOL JI Chem. Res. Toxicol. PD APR PY 2016 VL 29 IS 4 BP 438 EP 451 DI 10.1021/acs.chemrestox.5b00388 PG 14 WC Chemistry, Medicinal; Chemistry, Multidisciplinary; Toxicology SC Pharmacology & Pharmacy; Chemistry; Toxicology GA DJ9BJ UT WOS:000374508700002 PM 26686752 ER PT J AU Weidemann, E Andersson, PL Bidleman, T Boman, C Carlin, DJ Collina, E Cormier, SA Gouveia-Figueira, SC Gullett, BK Johansson, C Lucas, D Lundin, L Lundstedt, S Marklund, S Nording, ML Ortuno, N Sallam, AA Schmidt, FM Jansson, S AF Weidemann, Eva Andersson, Patrik L. Bidleman, Terry Boman, Christoffer Carlin, Danielle J. Collina, Elena Cormier, Stephania A. Gouveia-Figueira, Sandra C. Gullett, Brian K. Johansson, Christer Lucas, Donald Lundin, Lisa Lundstedt, Staffan Marklund, Stellan Nording, Malin L. Ortuno, Nuria Sallam, Asmaa A. Schmidt, Florian M. Jansson, Stina TI 14th congress of combustion by-products and their health effects-origin, fate, and health effects of combustion-related air pollutants in the coming era of bio-based energy sources SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Products of incomplete combustion; Human health; Soot; Particles; Polychlorinated dibenzo-p-dioxins; Polychlorinated dibenzofurans; Congress paper ID MASS-SPECTROMETRY; VIRUS-INFECTION; FLUE-GAS; WASTE; PYROLYSIS; DECOMPOSITION; EMISSION; LIGNINS; DIOXINS; CARBON AB The 14th International Congress on Combustion By-Products and Their Health Effects was held in UmeAyen, Sweden from June 14th to 17th, 2015. The Congress, mainly sponsored by the National Institute of Environmental Health Sciences Superfund Research Program and the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, focused on the "Origin, fate and health effects of combustion-related air pollutants in the coming era of bio-based energy sources". The international delegates included academic and government researchers, engineers, scientists, policymakers and representatives of industrial partners. The Congress provided a unique forum for the discussion of scientific advances in this research area since it addressed in combination the health-related issues and the environmental implications of combustion by-products. The scientific outcomes of the Congress included the consensus opinions that: (a) there is a correlation between human exposure to particulate matter and increased cardiac and respiratory morbidity and mortality; (b) because currently available data does not support the assessment of differences in health outcomes between biomass smoke and other particulates in outdoor air, the potential human health and environmental impacts of emerging air-pollution sources must be addressed. Assessment will require the development of new approaches to characterize combustion emissions through advanced sampling and analytical methods. The Congress also concluded the need for better and more sustainable e-waste management and improved policies, usage and disposal methods for materials containing flame retardants. C1 [Weidemann, Eva; Andersson, Patrik L.; Bidleman, Terry; Gouveia-Figueira, Sandra C.; Lundin, Lisa; Lundstedt, Staffan; Nording, Malin L.; Jansson, Stina] Umea Univ, Dept Chem, Umea, Sweden. [Boman, Christoffer; Schmidt, Florian M.] Umea Univ, Dept Appl Phys & Elect, Thermochem Energy Convers Lab, Umea, Sweden. [Carlin, Danielle J.] NIEHS, Dept Hlth & Human Serv, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Collina, Elena] Univ Milano Bicocca, Dept Earth & Environm Sci, Milan, Italy. [Cormier, Stephania A.; Sallam, Asmaa A.] Univ Tennessee, Ctr Hlth Sci, Dept Pediat, Memphis, TN 38163 USA. [Cormier, Stephania A.; Sallam, Asmaa A.] Le Bonheur Childrens Hosp, Childrens Fdn Res Inst, Memphis, TN USA. [Gullett, Brian K.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Johansson, Christer] Stockholm Univ, Dept Environm Sci & Analyt Chem, S-10691 Stockholm, Sweden. [Johansson, Christer] Environm & Hlth Adm, Stockholm, Sweden. [Lucas, Donald] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA. [Marklund, Stellan] Umea Univ, Bio4Energy, Umea, Sweden. [Ortuno, Nuria] Univ Alicante, Dept Chem Engn, E-03080 Alicante, Spain. RP Weidemann, E (reprint author), Umea Univ, Dept Chem, Umea, Sweden. EM eva.weidemann@umu.se RI Ortuno Garcia, Nuria/E-7127-2017; OI Ortuno Garcia, Nuria/0000-0003-0442-4890; Nording, Malin/0000-0002-1732-8147; Weidemann, Eva/0000-0001-5415-9330 FU National Institutes of Environmental Health Sciences; Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning-Formas [219-2014-291] FX The 14th Combustion By-Products and Their Health Effects Congress was supported by funds from National Institutes of Environmental Health Sciences to SC, and the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning-Formas (219-2014-291) to SJ, CB, MLN, LL, and EW. NR 23 TC 0 Z9 0 U1 9 U2 19 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD APR PY 2016 VL 23 IS 8 BP 8141 EP 8159 DI 10.1007/s11356-016-6308-y PG 19 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK5XN UT WOS:000374994600105 PM 26906006 ER PT J AU Lee, S Barron, MG AF Lee, Sehan Barron, Mace G. TI A mechanism-based 3D-QSAR approach for classification and prediction of acetylcholinesterase inhibitory potency of organophosphate and carbamate analogs SO JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN LA English DT Article DE Acetylcholinesterase inhibitor; 3D-QSAR; Mechanism-based; Molecular docking; Pharmacophore; 3D-fingerprint ID ACTIVE-SITE GORGE; IN-VITRO; MOLECULAR DOCKING; CRESYL PHOSPHATE; FLAME RETARDANTS; OXYANION HOLE; TOXICITY; EXPOSURE; BUTYRYLCHOLINESTERASE; CHOLINESTERASES AB Organophosphate (OP) and carbamate esters can inhibit acetylcholinesterase (AChE) by binding covalently to a serine residue in the enzyme active site, and their inhibitory potency depends largely on affinity for the enzyme and the reactivity of the ester. Despite this understanding, there has been no mechanism-based in silico approach for classification and prediction of the inhibitory potency of ether OPs or carbamates. This prompted us to develop a three dimensional prediction framework for OPs, carbamates, and their analogs. Inhibitory structures of a compound that can form the covalent bond were identified through analysis of docked conformations of the compound and its metabolites. Inhibitory potencies of the selected structures were then predicted using a previously developed three dimensional quantitative structure-active relationship. This approach was validated with a large number of structurally diverse OP and carbamate compounds encompassing widely used insecticides and structural analogs including OP flame retardants and thio- and dithiocarbamate pesticides. The modeling revealed that: (1) in addition to classical OP metabolic activation, the toxicity of carbamate compounds can be dependent on biotransformation, (2) OP and carbamate analogs such as OP flame retardants and thiocarbamate herbicides can act as AChEI, (3) hydrogen bonds at the oxyanion hole is critical for AChE inhibition through the covalent bond, and (4) pi-pi interaction with Trp86 is necessary for strong inhibition of AChE. Our combined computation approach provided detailed understanding of the mechanism of action of OP and carbamate compounds and may be useful for screening a diversity of chemical structures for AChE inhibitory potency. C1 [Lee, Sehan; Barron, Mace G.] US EPA, Gulf Ecol Div, Gulf Breeze, FL 32561 USA. RP Lee, S (reprint author), US EPA, Gulf Ecol Div, Gulf Breeze, FL 32561 USA. EM lee.sehan@epa.gov FU U.S. EPA; U.S. Department of Energy FX This research was supported in part by an appointment to the ORISE participant research program supported by an interagency agreement between the U.S. EPA and the U.S. Department of Energy. We thank Carlie LaLone for review of a draft of the manuscript. The conclusions may not necessarily reflect the views of EPA and no official endorsement should be inferred. NR 62 TC 3 Z9 3 U1 11 U2 33 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-654X EI 1573-4951 J9 J COMPUT AID MOL DES JI J. Comput.-Aided Mol. Des. PD APR PY 2016 VL 30 IS 4 BP 347 EP 363 DI 10.1007/s10822-016-9910-7 PG 17 WC Biochemistry & Molecular Biology; Biophysics; Computer Science, Interdisciplinary Applications SC Biochemistry & Molecular Biology; Biophysics; Computer Science GA DJ7SY UT WOS:000374413900007 PM 27055524 ER PT J AU Ding, DA Zhu, Y Jang, C Lin, CJ Wang, SX Fu, J Gao, J Deng, S Xie, JP Qiu, XZ AF Ding, Dian Zhu, Yun Jang, Carey Lin, Che-Jen Wang, Shuxiao Fu, Joshua Gao, Jian Deng, Shuang Xie, Junping Qiu, Xuezhen TI Evaluation of health benefit using BenMAP-CE with an integrated scheme of model and monitor data during Guangzhou Asian Games SO JOURNAL OF ENVIRONMENTAL SCIENCES LA English DT Article DE Air quality; Health benefit; PM2.5; BenMAP-CE; Data fusion; Model and monitor data ID PARTICULATE AIR-POLLUTION; ECONOMIC-ASSESSMENT; DAILY MORTALITY; URBAN AREAS; CHINA; SHANGHAI; BURDEN; MATTER; SECTORS; DISEASE AB Guangzhou is the capital and largest city (land area: 7287 km(2)) of Guangdong province in South China. The air quality in Guangzhou typically worsens in November due to unfavorable meteorological conditions for pollutant dispersion. During the Guangzhou Asian Games in November 2010, the Guangzhou government carried out a number of emission control measures that significantly improved the air quality. In this paper, we estimated the acute health outcome changes related to the air quality improvement during the 2010 Guangzhou Asian Games using a next-generation, fully-integrated assessment system for air quality and health benefits. This advanced system generates air quality data by fusing model and monitoring data instead of using monitoring data alone, which provides more reliable results. The air quality estimates retain the spatial distribution of model results while calibrating the value with observations. The results show that the mean PM2.5 concentration in November 2010 decreased by 3.5 mu g/m(3) compared to that in 2009 due to the emission control measures. From the analysis, we estimate that the air quality improvement avoided 106 premature deaths, 1869 cases of hospital admission, and 20,026 cases of outpatient visits. The overall cost benefit of the improved air quality is estimated to be 165 million CNY, with the avoided premature death contributing 90% of this figure. The research demonstrates that BenMAP-CE is capable of assessing the health and cost benefits of air pollution control for sound policy making. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. C1 [Ding, Dian; Zhu, Yun; Lin, Che-Jen; Xie, Junping; Qiu, Xuezhen] S China Univ Technol, Guangzhou Higher Educ Mega Ctr, Guangdong Prov Key Lab Atmospher Environm & Pollu, Coll Environm & Energy, Guangzhou 510006, Guangdong, Peoples R China. [Zhu, Yun; Wang, Shuxiao] State Environm Protect Key Lab Sources & Control, Beijing 100084, Peoples R China. [Jang, Carey] US EPA, Off Air Qual Planning & Stand, Res Triangle Pk, NC 27711 USA. [Lin, Che-Jen] Lamar Univ, Dept Civil Engn, Beaumont, TX 77710 USA. [Wang, Shuxiao] Tsinghua Univ, Dept Environm Sci & Engn, Beijing 100084, Peoples R China. [Fu, Joshua] Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA. [Gao, Jian; Deng, Shuang] Chinese Res Inst Environm Sci, Beijing 100012, Peoples R China. RP Zhu, Y (reprint author), S China Univ Technol, Guangzhou Higher Educ Mega Ctr, Guangdong Prov Key Lab Atmospher Environm & Pollu, Coll Environm & Energy, Guangzhou 510006, Guangdong, Peoples R China.; Zhu, Y (reprint author), State Environm Protect Key Lab Sources & Control, Beijing 100084, Peoples R China. EM zhuyun@scut.edu.cn RI wang, shuxiao/H-5990-2011; Lin, Che-Jen/K-1808-2013 OI wang, shuxiao/0000-0001-9727-1963; Lin, Che-Jen/0000-0001-5990-3093 FU US Environmental Protection Agency [5-312-0212979-51786L]; Guangzhou Environmental Protection Bureau [x2hjB2150020]; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control [2011A060901011]; project of Atmospheric Haze Collaboration Control Technology Design from the Chinese Academy of Sciences [XDB05030400]; National Environmental Protection Public Welfare Industry Targeted Research Foundation of China [201409019] FX Financial support and data source for this work were provided by the US Environmental Protection Agency (No. 5-312-0212979-51786L) and the Guangzhou Environmental Protection Bureau (No. x2hjB2150020), the project of an integrated modeling and filed observational verification on the deposition of typical industrial point-source mercury emissions in the Pearl River Delta. This work was also partly supported by the funding of the Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control (No. 2011A060901011), the project of Atmospheric Haze Collaboration Control Technology Design from the Chinese Academy of Sciences (No. XDB05030400), and the National Environmental Protection Public Welfare Industry Targeted Research Foundation of China (No. 201409019). NR 47 TC 0 Z9 0 U1 10 U2 17 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1001-0742 EI 1878-7320 J9 J ENVIRON SCI-CHINA JI J. Environ. Sci. PD APR PY 2016 VL 42 BP 9 EP 18 DI 10.1016/j.jes.2015.06.003 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK5RC UT WOS:000374976500003 PM 27090690 ER PT J AU Zhang, Y Shi, BY Zhao, YY Yan, MQ Lytle, DA Wang, DS AF Zhang, Yue Shi, Baoyou Zhao, Yuanyuan Yan, Mingquan Lytle, Darren A. Wang, Dongsheng TI Deposition behavior of residual aluminum in drinking water distribution system: Effect of aluminum speciation SO JOURNAL OF ENVIRONMENTAL SCIENCES LA English DT Article DE Aluminum deposition; Drinking water distribution system; Polyaluminum chloride (PACl); Quartz crystal microbalance ID QUARTZ-CRYSTAL MICROBALANCE; HUMIC-ACID; QCM-D; ADSORPTION; COAGULATION; DISSIPATION; STABILITY; SCALES; AL-30; RISK AB Finished drinking water usually contains some residual aluminum. The deposition of residual aluminum in distribution systems and potential release back to the drinking water could significantly influence the water quality at consumer taps. A preliminary analysis of aluminum content in cast iron pipe corrosion scales and loose deposits demonstrated that aluminum deposition on distribution pipe surfaces could be excessive for water treated by aluminum coagulants including polyaluminum chloride (PACl). In this work, the deposition features of different aluminum species in PACl were investigated by simulated coil-pipe test, batch reactor test and quartz crystal microbalance with dissipation monitoring. The deposition amount of non-polymeric aluminum species was the least, and its deposition layer was soft and hydrated, which indicated the possible formation of amorphous Al(OH)(3). Al-13 had the highest deposition tendency, and the deposition layer was rigid and much less hydrated, which indicated that the deposited aluminum might possess regular structure and self-aggregation of Al-13 could be the main deposition mechanism. While for Al-30, its deposition was relatively slower and deposited aluminum amount was relatively less compared with Al-13. However, the total deposited mass of Al-30 was much higher than that of Al-13, which was attributed to the deposition of particulate aluminum matters with much higher hydration state. Compared with stationary condition, stirring could significantly enhance the deposition process, while the effect of pH on deposition was relatively weak in the near neutral range of 6.7 to 8.7. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. C1 [Zhang, Yue; Shi, Baoyou; Zhao, Yuanyuan; Wang, Dongsheng] Chinese Acad Sci, Key Lab Drinking Water Sci & Technol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China. [Yan, Mingquan] Peking Univ, Coll Environm Sci & Engn, Beijing 100871, Peoples R China. [Lytle, Darren A.] US EPA, Cincinnati, OH 45268 USA. RP Shi, BY (reprint author), Chinese Acad Sci, Key Lab Drinking Water Sci & Technol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China. EM yuezhang12345@163.com; byshi@rcees.ac.cn FU National Natural Science Foundation of China [51378493, 51178450] FX The authors are very grateful to all personnel who provided support for this research. This work was supported by the National Natural Science Foundation of China (Nos. 51378493, and 51178450). NR 36 TC 2 Z9 3 U1 8 U2 26 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1001-0742 EI 1878-7320 J9 J ENVIRON SCI-CHINA JI J. Environ. Sci. PD APR PY 2016 VL 42 BP 142 EP 151 DI 10.1016/j.jes.2015.05.010 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK5RC UT WOS:000374976500017 PM 27090705 ER PT J AU Hofmeister, EK Jankowski, MD Goldberg, D Franson, JC AF Hofmeister, Erik K. Jankowski, Mark D. Goldberg, Diana Franson, J. Christian TI SURVEY FOR WEST NILE VIRUS ANTIBODIES IN WILD DUCKS, 2004-06, USA SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE American Wigeon; Duck; Mallard; Northern Pintail; serologic response; West Nile virus; Wood Duck ID LINKED-IMMUNOSORBENT-ASSAY; GREATER SAGE-GROUSE; DOMESTIC DUCKS; NORTH-AMERICA; UNITED-STATES; OUTBREAK; BIRDS; INFECTION; MORTALITY; ANAS AB Detection of West Nile virus (WNV) in ducks has been reported in North America in isolated cases of mortality in wild waterbirds and following outbreaks in farmed ducks. Although the virus has been noted as an apparent incidental finding in several species of ducks, little is known about the prevalence of exposure or the outcome of infection with WNV in wild ducks in North America. From 2004-06, we collected sera from 1,406 wild-caught American Wigeon (Anas americana), Mallard (Anas platyrhynchos), and Northern Pintail (Anas acuta) ducks at national wildlife refuges (NWRs) in North Dakota and Wood Ducks (Aix sponsa) at NWRs in South Carolina and Tennessee. We measured the prevalence of previous exposure to WNV in these ducks by measuring WNV antibodies and evaluated variation in exposure among species, age, and year. Additionally, we evaluated the performance of a commercial antibody to wild bird immunoglobulin in duck species that varied in their phylogenetic relatedness to the bird species the antibody was directed against. As determined by a screening immunoassay and a confirmatory plaque reduction neutralization assay, the prevalence of WNV antibody was 10%. In light of experimental studies that show ducks to be relatively resistant to mortality caused by WNV, the antibody prevalence we detected suggests that wild ducks may be less-frequently exposed to WNV than expected for birds inhabiting wetlands where they may acquire infection from mosquitoes. C1 [Hofmeister, Erik K.; Jankowski, Mark D.; Goldberg, Diana; Franson, J. Christian] US Geol Survey, Natl Wildlife Hlth Ctr, 6006 Schroeder Rd, Madison, WI 53711 USA. [Jankowski, Mark D.] US EPA, Off Environm Review & Assessment, Risk Evaluat Unit, 1200 6th Ave,Suite 900,OEA-140, Seattle, WA 98101 USA. RP Hofmeister, EK (reprint author), US Geol Survey, Natl Wildlife Hlth Ctr, 6006 Schroeder Rd, Madison, WI 53711 USA. EM ehofmeister@usgs.gov OI Goldberg, Diana/0000-0001-8540-8512 NR 39 TC 1 Z9 1 U1 1 U2 1 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD APR PY 2016 VL 52 IS 2 BP 354 EP 363 DI 10.7589/2015-06-137 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA DK8XP UT WOS:000375213100019 PM 26981693 ER PT J AU Keith, DJ Lunetta, RS Schaeffer, BA AF Keith, Darryl J. Lunetta, Ross S. Schaeffer, Blake A. TI Optical Models for Remote Sensing of Colored Dissolved Organic Matter Absorption and Salinity in New England, Middle Atlantic and Gulf Coast Estuaries USA SO REMOTE SENSING LA English DT Article DE CDOM absorption; salinity; MERIS; HICO; bio-optical models ID EAST CHINA SEA; OF-MEXICO; OCEAN COLOR; CLYDE SEA; CDOM; WATERS; LAKE; BAY; VARIABILITY; SCATTERING AB Ocean color algorithms have been successfully developed to estimate chlorophyll a and total suspended solids concentrations in coastal and estuarine waters but few have been created to estimate light absorption due to colored dissolved inorganic matter (CDOM) and salinity from the spectral signatures of these waters. In this study, we used remotely sensed reflectances in the red and blue-green portions of the visible spectrum retrieved from Medium Resolution Imaging Spectrometer (MERIS) and the International Space Station (ISS) Hyperspectral Imager for the Coastal Ocean (HICO) images to create a model to estimate CDOM absorption. CDOM absorption results were then used to develop an algorithm to predict the surface salinities of coastal bays and estuaries in New England, Middle Atlantic, and Gulf of Mexico regions. Algorithm-derived CDOM absorptions and salinities were successfully validated using laboratory measured absorption values over magnitudes of similar to 0.1 to 7.0 m(-1) and field collected CTD data from oligohaline to polyhaline (S less than 5 to 18-30) environments in Narragansett Bay (Rhode Island); the Neuse River Estuary (North Carolina); Pensacola Bay (Florida); Choctawhatchee Bay (Florida); St. Andrews Bay (Florida); St. Joseph Bay (Florida); and inner continental shelf waters of the Gulf of Mexico. C1 [Keith, Darryl J.] US EPA, Atlant Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Narragansett, RI 02882 USA. [Lunetta, Ross S.; Schaeffer, Blake A.] US EPA, Environm Measurements & Methods Div, Natl Exposure Res Lab, Off Res & Dev, Durham, NC 27709 USA. RP Keith, DJ (reprint author), US EPA, Atlant Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Narragansett, RI 02882 USA. EM keith.darryl@epa.gov; lunetta.ross@epa.gov; schaeffer.blake@epa.gov FU U.S. Environmental Protection Agency FX Although the research described in this manuscript has been funded by the U.S. Environmental Protection Agency, it has not been subjected to Agency review. Therefore, it does not necessarily reflect the views of the Agency. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. This manuscript is ORD tracking number ORD-014966. The authors declare no conflict of interest. Ross S. Lunetta (USEPA) and Blake A. Schaeffer (USEPA) provided field and laboratory data for the Neuse River (NC) and northwest Gulf of Mexico estuaries. Darryl J. Keith wrote the paper with significant input from Ross S. Lunetta and Blake A. Schaeffer and provided field and laboratory data for the Narragansett Bay estuary. We would like to thank Wayne Munns and Timothy Gleason (USEPA-Atlantic Ecology Division (AED)), Glen Thursby (USEPA-AED), Joseph LiVolsi (USEPA-AED), Jason Grear (USEPA-AED) and four anonymous reviewers for valuable comments and observations during manuscript preparation and revision. A special thanks is given to Doug Levin at the Center for Environment and Society (Washington College) for his comments and insight. NR 55 TC 0 Z9 0 U1 8 U2 19 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD APR PY 2016 VL 8 IS 4 AR 283 DI 10.3390/rs8040283 PG 22 WC Remote Sensing SC Remote Sensing GA DK8DD UT WOS:000375156500016 ER PT J AU Johnson, TAN Kaushal, SS Mayer, PM Smith, RM Sivirichi, GM AF Johnson, Tamara A. Newcomer Kaushal, Sujay S. Mayer, Paul M. Smith, Rose M. Sivirichi, Gwen M. TI Nutrient Retention in Restored Streams and Rivers: A Global Review and Synthesis SO WATER LA English DT Review DE stream restoration; floodplain; green infrastructure; nutrient spiraling; nutrient retention; nitrogen; phosphorus; denitrification; buried stream; sustainability; urban evolution ID ORGANIC-MATTER DYNAMICS; TRANSIENT STORAGE; URBAN STREAM; WATER-QUALITY; NITROGEN DYNAMICS; HEADWATER STREAMS; AMMONIUM UPTAKE; LAND-USE; MANAGEMENT STRATEGIES; ECOSYSTEM METABOLISM AB Excess nitrogen (N) and phosphorus (P) from human activities have contributed to degradation of coastal waters globally. A growing body of work suggests that hydrologically restoring streams and rivers in agricultural and urban watersheds has potential to increase N and P retention, but rates and mechanisms have not yet been analyzed and compared across studies. We conducted a review of nutrient retention within hydrologically reconnected streams and rivers, including 79 studies. We developed a typology characterizing different forms of stream and river restoration, and we also analyzed nutrient retention across this typology. The studies we reviewed used a variety of methods to analyze nutrient cycling. We performed a further intensive meta -analysis on nutrient spiraling studies because this method was the most consistent and comparable between studies. A meta -analysis of 240 experimental additions of ammonium (NH4+), nitrate (NO3), and soluble reactive phosphorus (SRP) was synthesized from 15 nutrient spiraling studies. Our results showed statistically significant relationships between nutrient uptake in restored streams and specific watershed attributes. Nitrate uptake metrics were significantly related to watershed surface area, impervious surface cover, and average reach width (p < 0.05). Ammonium uptake metrics were significantly related to discharge, velocity, and transient storage (p < 0.05). SRP uptake metrics were significantly related to watershed area, discharge, SRP concentrations, and chl a concentrations (p < 0.05). Given that most studies were conducted during baseflow, more research is necessary to characterize nutrient uptake during high flow. Furthermore, long-term studies are needed to understand changes in nutrient dynamics as projects evolve over time. Overall analysis suggests the size of the stream restoration (surface area), hydrologic connectivity, and hydrologic residence time are key drivers influencing nutrient retention at broader watershed scales and along the urban watershed continuum. C1 [Johnson, Tamara A. Newcomer] US EPA, Coastal Management Branch, Oceans & Coastal Protect Div, Washington, DC 20460 USA. [Johnson, Tamara A. Newcomer; Kaushal, Sujay S.; Smith, Rose M.] Univ Maryland, Dept Geol, College Pk, MD 21201 USA. [Johnson, Tamara A. Newcomer; Kaushal, Sujay S.; Smith, Rose M.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, Baltimore, MD 21201 USA. [Mayer, Paul M.] US EPA, Natl Hlth & Environm Effects Res Lab, Western Ecol Div, Corvallis, OR 97333 USA. [Sivirichi, Gwen M.] AKRF Inc, Hanover, MD 21076 USA. RP Johnson, TAN (reprint author), US EPA, Coastal Management Branch, Oceans & Coastal Protect Div, Washington, DC 20460 USA.; Johnson, TAN (reprint author), Univ Maryland, Dept Geol, College Pk, MD 21201 USA.; Johnson, TAN (reprint author), Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, Baltimore, MD 21201 USA. EM newcomer-johnson.tammy@epa.gov; skaushal@umd.edu; mayer.paul@epa.gov; rsmith24@umd.edu; gsivirichi@akrf.com RI Newcomer Johnson, Tamara/D-1045-2013; OI Newcomer Johnson, Tamara/0000-0002-2496-7641; Mayer, Paul/0000-0002-8550-1386 FU MD Sea Grant Awards [SA7528085-U, R/WS-2, NA05OAR4171042]; NSF [CBET 1058502, EAR 1426844, EAR 1521224]; EPA NNEMS Award [2010-308]; U.S. EPA Office of Research and Development; Baltimore Ecosystem Study LTER project [NSF DEB-0423476] FX This research was supported by MD Sea Grant Awards SA7528085-U, R/WS-2 and NA05OAR4171042, NSF Awards CBET 1058502, EAR 1426844, and EAR 1521224, EPA NNEMS Award 2010-308, the U.S. EPA Office of Research and Development, and Baltimore Ecosystem Study LTER project (NSF DEB-0423476). We thank Michael Pennino, Walter Boynton, Peter Groffman, Jeff Cornwell, Philippe Vidon, Jim Markwiese, and three anonymous reviewers for improving earlier drafts. We thank Shuiwang Duan for help with translation from Chinese to English. Melanie Harrison Okoro, Jonathan Jeschke, and Michael Craghan provided valuable discussions. The information in this document has been subjected to Agency peer and administrative review, and it has been approved for publication as an Agency document. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 107 TC 2 Z9 2 U1 44 U2 78 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4441 J9 WATER-SUI JI Water PD APR PY 2016 VL 8 IS 4 AR 116 DI 10.3390/w8040116 PG 28 WC Water Resources SC Water Resources GA DK8DK UT WOS:000375157200007 ER PT J AU Xue, XB Hawkins, TR Schoen, ME Garland, J Ashbolt, NJ AF Xue, Xiaobo Hawkins, Troy R. Schoen, Mary E. Garland, Jay Ashbolt, Nicholas J. TI Comparing the Life Cycle Energy Consumption, Global Warming and Eutrophication Potentials of Several Water and Waste Service Options SO WATER LA English DT Article DE Life cycle assessment; water service; sanitation service; energy; greenhouse gas emission; nutrient ID SOURCE-SEPARATION SYSTEMS; URBAN WATER; ANAEROBIC TREATMENT; TREATMENT PLANTS; COMMUNITY WATER; EMBODIED ENERGY; SUPPLY-SYSTEMS; MANAGEMENT; TECHNOLOGIES; IMPACTS AB Managing the water-energy-nutrient nexus for the built environment requires, in part, a full system analysis of energy consumption, global warming and eutrophication potentials of municipal water services. As an example, we evaluated the life cycle energy use, greenhouse gas (GHG) emissions and aqueous nutrient releases of the whole anthropogenic municipal water cycle starting from raw water extraction to wastewater treatment and reuse/discharge for five municipal water and wastewater systems. The assessed options included conventional centralized services and four alternative options following the principles of source-separation and water fit-for-purpose. The comparative life cycle assessment identified that centralized drinking water supply coupled with blackwater energy recovery and on-site greywater treatment and reuse was the most energy- and carbon-efficient water service system evaluated, while the conventional (drinking water and sewerage) centralized system ranked as the most energy-and carbon-intensive system. The electricity generated from blackwater and food residuals co-digestion was estimated to offset at least 40% of life cycle energy consumption for water/waste services. The dry composting toilet option demonstrated the lowest life cycle eutrophication potential. The nutrients in wastewater effluent are the dominating contributors for the eutrophication potential for the assessed system configurations. Among the parameters for which variability and sensitivity were evaluated, the carbon intensity of the local electricity grid and the efficiency of electricity production by the co-digestion with the energy recovery process were the most important for determining the relative global warming potential results. C1 [Xue, Xiaobo] SUNY Albany, Dept Environm Hlth Sci, Rensselaer, NY 12144 USA. [Hawkins, Troy R.] Enviance, 5780 Fleet St,Suite 200, Carlsbad, CA 92008 USA. [Schoen, Mary E.] Soller Environm Inc, 3022 King St, Berkeley, CA 94703 USA. [Garland, Jay] US EPA, Off Res & Dev, Natl Exposure Res Lab, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Ashbolt, Nicholas J.] Univ Alberta, Sch Publ Hlth, Edmonton, AB T6G 2G7, Canada. RP Xue, XB (reprint author), SUNY Albany, Dept Environm Hlth Sci, Rensselaer, NY 12144 USA. EM xxue@albany.edu; trh@alumni.cmu.edu; mschoen@sollerenvironmental.com; Garland.Jay@epa.gov; ashbolt@ualberta.ca OI Ashbolt, Nicholas/0000-0002-3853-0096 FU U.S. Environmental Protection Agency Office of Research and Development through the ORISE Post-Doctoral Fellowship Program; Faculty Research Award Program of the State University of New York at Albany; Individual Development Awards Program of the State University of New York at Albany FX This project was supported by the U.S. Environmental Protection Agency Office of Research and Development through the ORISE Post-Doctoral Fellowship Program and other project-related support. This effort was also supported by the Faculty Research Award Program and Individual Development Awards Program of the State University of New York at Albany. The senior author (NA) would like to acknowledge many years of discussions and recent data provided by Greg Peters (University of New South Wales and Chalmers University) and Sven Lundie (University of New South Wales and PE International Hamburg) and Hilder Maingay and Earle Barnhart of Falmouth. The authors also would like to acknowledge Jennifer Cashdollar and Michael Gonzales and Cissy Ma (U.S. Environmental Protection Agency) for their insightful comments and suggestions. The views expressed in this article are those of the authors and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency. Any mention of specific products or processes does not represent endorsement by the U.S. Environmental Protection Agency. NR 77 TC 2 Z9 2 U1 16 U2 36 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4441 J9 WATER-SUI JI Water PD APR PY 2016 VL 8 IS 4 AR 154 DI 10.3390/w8040154 PG 21 WC Water Resources SC Water Resources GA DK8DK UT WOS:000375157200045 ER PT J AU Yen, H Daggupati, P White, MJ Srinivasan, R Gossel, A Wells, D Arnold, JG AF Yen, Haw Daggupati, Prasad White, Michael J. Srinivasan, Raghavan Gossel, Arndt Wells, David Arnold, Jeffrey G. TI Application of Large-Scale, Multi-Resolution Watershed Modeling Framework Using the Hydrologic and Water Quality System (HAWQS) SO WATER LA English DT Article DE decision support system; watershed modeling; web-based application; model calibration; SWAT ID ASSESSMENT-TOOL; CALIBRATION; VALIDATION; SIMULATIONS; IMPACT; SWAT AB In recent years, large-scale watershed modeling has been implemented broadly in the field of water resources planning and management. Complex hydrological, sediment, and nutrient processes can be simulated by sophisticated watershed simulation models for important issues such as water resources allocation, sediment transport, and pollution control. Among commonly adopted models, the Soil and Water Assessment Tool (SWAT) has been demonstrated to provide superior performance with a large amount of referencing databases. However, it is cumbersome to perform tedious initialization steps such as preparing inputs and developing a model with each changing targeted study area. In this study, the Hydrologic and Water Quality System (HAWQS) is introduced to serve as a national-scale Decision Support System (DSS) to conduct challenging watershed modeling tasks. HAWQS is a web-based DSS developed and maintained by Texas A & M University, and supported by the U.S. Environmental Protection Agency. Three different spatial resolutions of Hydrologic Unit Code (HUC8, HUC10, and HUC12) and three temporal scales (time steps in daily/monthly/annual) are available as alternatives for general users. In addition, users can specify preferred values of model parameters instead of using the pre-defined sets. With the aid of HAWQS, users can generate a preliminarily calibrated SWAT project within a few minutes by only providing the ending HUC number of the targeted watershed and the simulation period. In the case study, HAWQS was implemented on the Illinois River Basin, USA, with graphical demonstrations and associated analytical results. Scientists and/or decision-makers can take advantage of the HAWQS framework while conducting relevant topics or policies in the future. C1 [Yen, Haw] Texas A&M Univ, Blackland Res & Extens Ctr, Texas A&M Agrilife Res, 720 East Blackland Rd, Temple, TX 76502 USA. [Daggupati, Prasad; Srinivasan, Raghavan] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. [White, Michael J.; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. [Gossel, Arndt] US EPA, Oak Ridge Inst Sci & Educ, Off Water, Washington, DC 20460 USA. [Wells, David] US EPA, Off Water, Washington, DC 20460 USA. RP Yen, H (reprint author), Texas A&M Univ, Blackland Res & Extens Ctr, Texas A&M Agrilife Res, 720 East Blackland Rd, Temple, TX 76502 USA. EM haw.yen@gmail.com; pdaggupati@tamu.edu; mike.white@ars.usda.gov; r-srinivasan@tamu.edu; gossel.arndt@epa.gov; dwells@his.com; jeff.arnold@ars.usda.gov RI Srinivasan, R/D-3937-2009; Daggupati, Prasad/D-8886-2017 OI Daggupati, Prasad/0000-0002-7044-3435 FU U.S. Environmental Protection Agency; United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP)-Wildlife and Cropland components FX This project was funded by grants from (i) U.S. Environmental Protection Agency; and (ii) United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP)-Wildlife and Cropland components. The views expressed in this presentation are those of the authors and do not necessarily reflect the views or policies of the United States Environmental Protection Agency and the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 38 TC 3 Z9 3 U1 5 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4441 J9 WATER-SUI JI Water PD APR PY 2016 VL 8 IS 4 AR 164 DI 10.3390/w8040164 PG 23 WC Water Resources SC Water Resources GA DK8DK UT WOS:000375157200055 ER PT J AU Greenlee, H Molmenti, CLS Falci, L Ulmer, R Deming-Halverson, S DeRoo, LA Sandler, DP AF Greenlee, Heather Molmenti, Christine L. Sardo Falci, Laura Ulmer, Ross Deming-Halverson, Sandra DeRoo, Lisa A. Sandler, Dale P. TI High use of complementary and alternative medicine among a large cohort of women with a family history of breast cancer: the Sister Study SO BREAST CANCER RESEARCH AND TREATMENT LA English DT Article DE Complementary and alternative medicine; Breast cancer; Breast cancer risk; Gail model ID LIFE-STYLE BEHAVIORS; NATIONAL-SURVEY; OVARIAN-CANCER; HIGH-RISK; WHITE; INFORMATION; CHOICES; CAM AB Use of complementary and alternative medicine (CAM) is high among U.S. women, yet information is limited on use among women at increased breast cancer risk. We analyzed CAM use among women with a family history of breast cancer. CAM use was analyzed among women enrolled 2003-2009 in the Sister Study cohort. Eligible women were aged 35-74, U.S. or Puerto Rican residents, no personal history of breast cancer, and had a parts per thousand yen1 sister with breast cancer. Baseline data on CAM use in the past year were available for 49,734 women. Logistic regression models examined the association between CAM use and Gail Model breast cancer risk score. Results were compared to female participants in the 2007 National Health Interview Survey (n = 7965). Among Sister Study participants, there was high use of vitamin/mineral supplements (79 %), mind-body practices (41 %), manipulative/body-based practices (32 %), and botanicals (23 %). Overall use was higher than the U.S. female population. No association was observed between familial breast cancer risk and CAM use. Black women were more likely to use spirituality/meditation-based CAM modalities, while non-Hispanic white and Asian women were high users of dietary supplements. In a cohort of women with increased breast cancer risk due to family history, CAM use is higher than women in the general U.S. population and is associated with race/ethnicity. Use was not associated with breast cancer risk. Given the high prevalence of CAM use among women at risk for breast caner, research on the effectiveness of CAM use for disease prevention is needed. C1 [Greenlee, Heather; Molmenti, Christine L. Sardo; Falci, Laura] Columbia Univ, Mailman Sch Publ Hlth, 722 W 168th St, New York, NY 10032 USA. [Greenlee, Heather; Molmenti, Christine L. Sardo] Columbia Univ, Herbert Irving Comprehens Canc Ctr, New York, NY USA. [Ulmer, Ross] WESTAT Corp, Durham, NC USA. [Deming-Halverson, Sandra] Social & Sci Syst Inc, Durham, NC USA. [Deming-Halverson, Sandra] Vanderbilt Univ, Sch Med, Div Epidemiol, Nashville, TN 37212 USA. [DeRoo, Lisa A.; Sandler, Dale P.] Natl Inst Environm Hlth Sci, Res Triangle Pk, Durham, NC USA. [DeRoo, Lisa A.] Univ Bergen, Dept Global Publ Hlth & Primary Care, Bergen, Norway. RP Greenlee, H (reprint author), Columbia Univ, Mailman Sch Publ Hlth, 722 W 168th St, New York, NY 10032 USA.; Greenlee, H (reprint author), Columbia Univ, Herbert Irving Comprehens Canc Ctr, New York, NY USA. EM hg2120@columbia.edu OI Sandler, Dale/0000-0002-6776-0018 FU National Cancer Institute at the National Institutes of Health [K23CA141052, T32CA009529-25]; Intramural Research Program of the NIH, National Institute of Environmental Health Science [Z1A ES044005] FX Funding was provided by the National Cancer Institute at the National Institutes of Health (Grants K23CA141052 to H.G. and T32CA009529-25 to C.S.M.) and the Intramural Research Program of the NIH, National Institute of Environmental Health Science (Z1A ES044005). NR 32 TC 0 Z9 0 U1 8 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0167-6806 EI 1573-7217 J9 BREAST CANCER RES TR JI Breast Cancer Res. Treat. PD APR PY 2016 VL 156 IS 3 BP 527 EP 538 DI 10.1007/s10549-016-3740-0 PG 12 WC Oncology SC Oncology GA DK1IU UT WOS:000374666500014 PM 27017506 ER PT J AU Gordon, CJ Phillips, PM Johnstone, AFM Beasley, TE Ledbetter, AD Schladweiler, MC Snow, SJ Kodavanti, UP AF Gordon, C. J. Phillips, P. M. Johnstone, A. F. M. Beasley, T. E. Ledbetter, A. D. Schladweiler, M. C. Snow, S. J. Kodavanti, U. P. TI Effect of high-fructose and high-fat diets on pulmonary sensitivity, motor activity, and body composition of brown Norway rats exposed to ozone SO INHALATION TOXICOLOGY LA English DT Article DE Air pollution; BALF biomarkers; food consumption; metabolism; obesitypenH ID INNATE AIRWAY HYPERRESPONSIVENESS; RESPIRATORY HEALTH; SUBACUTE OZONE; DEFICIENT MICE; CORN SYRUP; OBESITY; POLLUTION; RESPONSES; RESPONSIVENESS; INFLAMMATION AB Diet-induced obesity has been suggested to lead to increased susceptibility to air pollutants such as ozone (O-3); however, there is little experimental evidence. Thirty day old male and female Brown Norway rats were fed a normal, high-fructose or high-fat diet for 12 weeks and then exposed to O-3 (acute - air or 0.8ppm O-3 for 5 h, or subacute - air or 0.8ppm O-3 for 5 h/d 1 d/week for 4 weeks). Body composition was measured non-invasively using NMR. Ventilatory parameters and exploratory behavior were measured after the third week of subacute exposure. Bronchoalveolar lavage fluid (BALF) and blood chemistry data were collected 18 h after acute O-3 and 18 h after the fourth week of subacute O-3. The diets led to increased body fat in male but not female rats. O-3-induced changes in ventilatory function were either unaffected or improved with the fructose and fat diets. O-3-induced reduction in exploratory behavior was attenuated with fructose and fat diets in males and partially in females. O-3 led to a significant decrease in body fat of males fed control diet but not the fructose or fat diet. O-3 led to significant increases in BALF eosinophils, increase in albumin, and reductions in macrophages. Female rats appeared to be more affected than males to O-3 regardless of diet. Overall, treatment with high-fructose and high-fat diets attenuated some O-3 induced effects on pulmonary function, behavior, and metabolism. Exacerbation of toxicity was observed less frequently. C1 [Gordon, C. J.; Phillips, P. M.; Johnstone, A. F. M.; Beasley, T. E.] Tox Assessment Div, Durham, NC USA. [Ledbetter, A. D.; Schladweiler, M. C.; Snow, S. J.; Kodavanti, U. P.] US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, Durham, NC USA. RP Gordon, CJ (reprint author), US EPA, Res Triangle Pk, Durham, NC USA. EM gordon.christopher@epa.gov OI Snow, Samantha/0000-0003-1812-8582 NR 27 TC 2 Z9 2 U1 3 U2 5 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0895-8378 EI 1091-7691 J9 INHAL TOXICOL JI Inhal. Toxicol. PD APR PY 2016 VL 28 IS 5 BP 203 EP 215 DI 10.3109/08958378.2015.1134730 PG 13 WC Toxicology SC Toxicology GA DK0VX UT WOS:000374631400001 PM 27092583 ER PT J AU Nover, DM Witt, JW Butcher, JB Johnson, TE Weaver, CP AF Nover, D. M. Witt, J. W. Butcher, J. B. Johnson, T. E. Weaver, C. P. TI The Effects of Downscaling Method on the Variability of Simulated Watershed Response to Climate Change in Five US Basins SO EARTH INTERACTIONS LA English DT Article DE Climate prediction; Ensembles; Models and modeling; Coupled models; Hydrologic models ID PROJECTIONS; MODELS; UNCERTAINTY; IMPACTS; ENSEMBLE AB Simulations of future climate change impacts on water resources are subject to multiple and cascading uncertainties associated with different modeling and methodological choices. A key facet of this uncertainty is the coarse spatial resolution of GCM output compared to the finer-resolution information needed by water managers. To address this issue, it is now common practice to apply spatial downscaling techniques, using either higher-resolution regional climate models or statistical approaches applied to GCM output, to develop finer-resolution information. Downscaling, however, can also introduce its own uncertainties into water resources' impact assessments. This study uses watershed simulations in five U.S. basins to quantify the sources of variability in streamflow, nitrogen, phosphorus, and sediment loads associated with the underlying GCM compared to the choice of downscaling method (both statistically and dynamically downscaled GCM output). This study also assesses the specific, incremental effects of downscaling by comparing watershed simulations based on downscaled and nondownscaled GCM model output. Results show that the underlying GCM and the downscaling method each contribute to the variability of simulated watershed responses. The relative contribution of GCM and downscaling method to the variability of simulated responses varies by watershed and season of the year. Results illustrate the potential implications of one key methodological choice in conducting climate change impact assessments for water-the selection of downscaled climate change information. C1 [Nover, D. M.] US Agcy Int Dev, Amer Assoc Adv Sci, Accra, Ghana. [Witt, J. W.; Johnson, T. E.] US EPA, Off Res & Dev, Washington, DC 20460 USA. [Butcher, J. B.] Tetra Tech Inc, Res Triangle Pk, NC USA. [Weaver, C. P.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Nover, DM (reprint author), US Agcy Int Dev, Accra, Ghana. EM dmnover@gmail.com RI Weaver, Christopher/G-3714-2010 OI Weaver, Christopher/0000-0003-4016-5451 FU National Science Foundation FX The authors thank the entire project team at Tetra Tech, Inc., Texas A&M University, AQUA TERRA, Stratus Consulting, and FTN Associates for their support contributing to the development of the watershed model simulations used in this analysis. We also thank Seth McGinnis of the National Center for Atmospheric Research (NCAR) for processing the North American Regional Climate Change Assessment Program (NARCCAP) output into change statistics for use in the watershed modeling. NCAR is supported by the National Science Foundation. We acknowledge the modeling groups, the Program for Climate Model Diagnosis and Intercomparison, and the WCRP's Working Group on Coupled Modeling for their roles in making available the WCRP Coupled Model Intercomparison Project Phase 3 (CMIP3) multimodel dataset. Support of this dataset is provided by the Office of Science, U.S. Department of Energy. The views expressed in this paper represent those of the authors and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency. NR 45 TC 1 Z9 1 U1 1 U2 5 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 1087-3562 J9 EARTH INTERACT JI Earth Interact. PD APR PY 2016 VL 20 AR 11 DI 10.1175/EI-D-15-0024.1 PG 27 WC Geosciences, Multidisciplinary SC Geology GA DJ3MJ UT WOS:000374109300001 ER PT J AU Yurista, PM Kelly, JR Scharold, JV AF Yurista, Peder M. Kelly, John R. Scharold, Jill V. TI Great Lakes nearshore-offshore: Distinct water quality regions SO JOURNAL OF GREAT LAKES RESEARCH LA English DT Article DE Nearshore; Offshore; Water quality; National Coastal Condition Assessment; Great Lakes ID AMPHIPOD DIPOREIA; BOUNDARY-LAYER; ONTARIO; ERIE; SUPERIOR; MICHIGAN; COASTAL; VARIABILITY; MANAGEMENT; CHEMISTRY AB We compared water quality of nearshore regions in the Laurentian Great Lakes to water quality in offshore regions. Sample sites for the nearshore region were from the US EPA National Coastal Condition Assessment and based on a criteria or sample-frame of within the 30-m depth contour or 5-km distance from the shoreline, whichever occurred first. The offshore sites were composed of US EPA Great Lakes National Program Office (GLNPO) annual monitoring sites. There was a contrast in both variability and mean values of water quality values between the nearshore and the offshore regions. Lake-by-lake the nearshore was more variable and had higher average parameter values than the offshore, except for NO2/3 which was lower in mean value. A subset of all sites was re-visited in supplemental years to explore temporal effects (57 nearshore sites in Lake Erie 2009 and for 67 nearshore sites in Lake Huron 2012). The operational sample-frame for nearshore water provided a reliable means for separating the lakes into two distinct and persistent water quality regions across years. Published by Elsevier B.V. on behalf of International Association for Great Lakes Research. C1 [Yurista, Peder M.; Kelly, John R.; Scharold, Jill V.] US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, 6201 Congdon Blvd, Duluth, MN 55804 USA. RP Yurista, PM (reprint author), US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, 6201 Congdon Blvd, Duluth, MN 55804 USA. EM woollybugger@charter.net; jacicrussellkelly@gmail.com; scharold.jill@epa.gov FU US EPA FX This work was funded entirely by the US EPA. We thank the EPA Office of Water and the EPA Great Lakes National Program Office for access to data used in this paper. We would like to thank Beth Hinchey-Malloy for comments and helpful suggestions. The views expressed in this article are those of the authors and do not necessarily reflect the views or policies of the US EPA. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 58 TC 0 Z9 0 U1 12 U2 19 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0380-1330 J9 J GREAT LAKES RES JI J. Gt. Lakes Res. PD APR PY 2016 VL 42 IS 2 BP 375 EP 385 DI 10.1016/j.jglr.2015.12.002 PG 11 WC Environmental Sciences; Limnology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DI7AI UT WOS:000373651100023 ER PT J AU McDowell, RW Dils, RM Collins, AL Flahive, KA Sharpley, AN Quinn, J AF McDowell, R. W. Dils, R. M. Collins, A. L. Flahive, K. A. Sharpley, A. N. Quinn, J. TI A review of the policies and implementation of practices to decrease water quality impairment by phosphorus in New Zealand, the UK, and the US SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE Algae; Critical source areas; Phosphorus; Profit; Production; Farming systems ID CONSERVATION PRACTICES; MITIGATION METHODS; COST-EFFECTIVENESS; DIFFUSE POLLUTION; DAIRY FARMS; LAKE-ERIE; BASE-LINE; MANAGEMENT; CATCHMENT; SURFACE AB The improper use of phosphorus (P) on agricultural land in developed countries is related to P losses that impair surface water quality. We outline policy in New Zealand, the UK, and the US who have imposed limits for P measured as ecological status, but in some cases, also as chemical concentrations or loads. We contrast the strategies used in each country and discuss their likelihood of being able to decrease P losses and improve surface water quality. All three countries have focused on understanding pathways and catchment processes so that cause and effect can be traced across spatial and temporal scales. A poor understanding of catchment processes and critical source areas of P loss has resulted in some areas where regulation has had minimal effect on P discharges. Furthermore, while biophysical science can inform policy, we give several examples where social and economic challenges are of equal if not greater relevance to P discharges (e.g. subsidies). Some evidence shows that these challenges can be overcome at the farm to small catchment scale with a mix of mandatory and voluntary rules in targeted areas. Other policy instruments (e.g. trading schemes) may be needed at larger scales, but should be flexible and encourage innovation over a culture of dependence. There is increasing recognition among all three countries that while targeting good management practices can substantially decrease P losses from existing land use, to achieve 'good' water quality in catchment, policy may have to consider land use change. C1 [McDowell, R. W.] AgResearch, Invermay Agr Ctr, Private Bag 50034, Mosgiel 9053, New Zealand. [McDowell, R. W.] Lincoln Univ, Agr & Life Sci, Lincoln 7647, New Zealand. [Dils, R. M.] Environm Agcy, Red Kite House,Howbery Pk, Wallingford OX10 8BD, Oxon, England. [Collins, A. L.] Rothamsted Res, Sustainable Soils & Grassland Syst Dept, North Wyke EX20 2SB, Okehampton, England. [Flahive, K. A.] US EPA, 1200 Penn Ave, Washington, DC 20460 USA. [Sharpley, A. N.] Univ Arkansas, Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Quinn, J.] Natl Inst Water & Atmospher Res, Gate 10,Silverdale Rd, Hamilton 3216, New Zealand. RP McDowell, RW (reprint author), AgResearch, Invermay Agr Ctr, Private Bag 50034, Mosgiel 9053, New Zealand. EM richard.mcdowell@agresearch.co.nz OI McDowell, Richard/0000-0003-3911-4825 FU Zealand Ministry for Business, Innovation and Employment's-Clean Water, Productive Land programme [C10X1006] FX Funding for RW McDowell was provided by the New Zealand Ministry for Business, Innovation and Employment's-Clean Water, Productive Land programme (C10X1006). The views expressed in this paper are those of the authors and do not reflect the official policy of the US Environmental Protection Agency nor the UK Environment Agency. This paper was iniated following meetings of the Phosphorus Sustainability Research Coordination Network (P RCN). NR 61 TC 3 Z9 3 U1 16 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 EI 1573-0867 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD APR PY 2016 VL 104 IS 3 SI SI BP 289 EP 305 DI 10.1007/s10705-015-9727-0 PG 17 WC Soil Science SC Agriculture GA DJ3LF UT WOS:000374106000004 ER PT J AU Verma, S Baig, RBN Nadagouda, MN Varma, RS AF Verma, Sanny Baig, R. B. Nasir Nadagouda, Mallikarjuna N. Varma, Rajender S. TI Photocatalytic C-H Activation of Hydrocarbons over VO@g-C3N4 SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE C-H activation; Graphitic carbon nitride; Photocatalyst; Vanadium oxide; Heterogeneous catalysis ID GRAPHITIC CARBON NITRIDE; COMPOSITE PHOTOCATALYSTS; SELECTIVE OXIDATION; HYDROGEN-PEROXIDE; SOLVENT-FREE; TOLUENE; GRAPHENE; BONDS; BENZALDEHYDE; DERIVATIVES AB A highly selective and sustainable method has been developed for the oxidation of methyl arenes and their analogues. The VO@g-C3N4 catalyst is very efficient in the C-H activation and oxygen insertion reaction resulting in formation of the corresponding carbonyl compounds and phenols. C1 [Verma, Sanny; Baig, R. B. Nasir; Varma, Rajender S.] US EPA, Sustainable Technol Div, Natl Risk Management Res Lab, 26 West Martin Luther King Dr,MS 443, Cincinnati, OH 45268 USA. [Nadagouda, Mallikarjuna N.] US EPA, WQMB, WSWRD, Natl Risk Management Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. RP Varma, RS (reprint author), US EPA, Sustainable Technol Div, Natl Risk Management Res Lab, 26 West Martin Luther King Dr,MS 443, Cincinnati, OH 45268 USA. EM varma.rajender@epa.gov FU National Risk Management Research Laboratory FX S.V. and R.B.N.B. were supported by the Postgraduate Research Program at the National Risk Management Research Laboratory administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Environmental Protection Agency. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. Any mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 23 TC 5 Z9 5 U1 23 U2 56 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD APR PY 2016 VL 4 IS 4 BP 2333 EP 2336 DI 10.1021/acssuschemeng.5b00006 PG 4 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DI5QV UT WOS:000373554600053 ER PT J AU Wood, JP Wendling, M Richter, W Lastivka, A Mickelsen, L AF Wood, Joseph P. Wendling, Morgan Richter, William Lastivka, Andrew Mickelsen, Leroy TI Evaluation of the Efficacy of Methyl Bromide in the Decontamination of Building and Interior Materials Contaminated with Bacillus anthracis Spores SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID GEOBACILLUS-STEAROTHERMOPHILUS SPORES; INACTIVATION; SUBTILIS; FUMIGANT; SURFACES AB The primary goal of this study was to determine the conditions required for the effective inactivation of Bacillus anthracis spores on materials by using methyl bromide (MeBr) gas. Another objective was to obtain comparative decontamination efficacy data with three avirulent microorganisms to assess their potential for use as surrogates for B. anthracis Ames. Decontamination tests were conducted with spores of B. anthracis Ames and Geobacillus stearothermophilus, B. anthracis NNR1 Delta 1, and B. anthracis Sterne inoculated onto six different materials. Experimental variables included temperature, relative humidity (RH), MeBr concentration, and contact time. MeBr was found to be an effective decontaminant under a number of conditions. This study highlights the important role that RH has when fumigation is performed with MeBr. There were no tests in which a >= 6-log(10) reduction (LR) of B. anthracis Ames was achieved on all materials when fumigation was done at 45% RH. At 75% RH, an increase in the temperature, the MeBr concentration, or contact time generally improved the efficacy of fumigation with MeBr. This study provides new information for the effective use of MeBr at temperatures and RH levels lower than those that have been recommended previously. The study also provides data to assist with the selection of an avirulent surrogate for B. anthracis Ames spores when additional tests with MeBr are conducted. C1 [Wood, Joseph P.] US EPA, Off Res & Dev, Natl Homeland Secur Res Ctr, Res Triangle Pk, NC 27711 USA. [Wendling, Morgan; Richter, William; Lastivka, Andrew] Battelle Mem Inst, West Jefferson, OH USA. [Mickelsen, Leroy] US EPA, Off Emergency Management, Res Triangle Pk, NC 27711 USA. RP Wood, JP (reprint author), US EPA, Off Res & Dev, Natl Homeland Secur Res Ctr, Res Triangle Pk, NC 27711 USA. EM wood.joe@epa.gov OI Wood, Joseph/0000-0001-6316-9418 FU U.S. Environmental Protection Agency through its Office of Research and Development [EP-C-10-001]; Battelle FX There was no grant associated with this work. The U.S. Environmental Protection Agency through its Office of Research and Development funded and directed the research described herein under contract number EP-C-10-001 with Battelle. The funder had a key role in study design, data interpretation, writing the manuscript, and the decision to submit the work for publication. NR 23 TC 0 Z9 0 U1 0 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD APR PY 2016 VL 82 IS 7 BP 2003 EP 2011 DI 10.1128/AEM.03445-15 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DI2QV UT WOS:000373342400003 PM 26801580 ER PT J AU Lin, CJ Wade, TJ Sams, EA Dufour, AP Chapman, AD Hilborn, ED AF Lin, Cynthia J. Wade, Timothy J. Sams, Elizabeth A. Dufour, Alfred P. Chapman, Andrew D. Hilborn, Elizabeth D. TI A Prospective Study of Marine Phytoplankton and Reported Illness Among Recreational Beachgoers in Puerto Rico, 2009 SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID HARMFUL ALGAL BLOOMS; BLUE-GREEN-ALGAE; RED TIDE EVENTS; CLIMATE-CHANGE; LYNGBYA-MAJUSCULA; UNITED-STATES; HUMAN HEALTH; AEROSOLIZED BREVETOXINS; WATERBORNE DISEASE; PROSPECTIVE COHORT AB BACKGROUND: Blooms of marine phytoplankton may adversely affect human health. The potential public health impact of low-level exposures is not well established, and few prospective cohort studies of recreational exposures to marine phytoplankton have been conducted. OBJECTIVE: We evaluated the association between phytoplankton cell counts and subsequent illness among recreational beachgoers. METHODS: We recruited beachgoers at Boqueron Beach, Puerto Rico, during the summer of 2009. We conducted interviews at three time points to assess baseline health, water activities, and subsequent illness. Daily water samples were quantitatively assayed for phytoplankton cell count. Logistic regression models, adjusted for age and sex, were used to assess the association between exposure to three categories of phytoplankton concentration and subsequent illness. RESULTS: During 26 study days, 15,726 individuals successfully completed all three interviews. Daily total phytoplankton cell counts ranged from 346 to 2,012cells/mL (median, 712cells/mL). The category with the highest (= >= 75th percentile) total phytoplankton cell count was associated with eye irritation [adjusted odds ratio (OR) = 1.30; 95% confidence interval (CI): 1.01, 1.66], rash (OR = 1.27; 95% CI: 1.02, 1.57), and earache (OR = 1.25; 95% CI: 0.88, 1.77). In phytoplankton group-specific analyses, the category with the highest Cyanobacteria counts was associated with respiratory illness (OR = 1.37; 95% CI: 1.12, 1.67), rash (OR = 1.32; 95% CI: 1.05, 1.66), eye irritation (OR = 1.25; 95% CI: 0.97, 1.62), and earache (OR = 1.35; 95% CI: 0.95, 1.93). CONCLUSIONS: We found associations between recreational exposure to marine phytoplankton and reports of eye irritation, respiratory illness, and rash. We also found that associations varied by phytoplankton group, with Cyanobacteria having the strongest and most consistent associations. C1 [Lin, Cynthia J.] US EPA, Res Participat Program, ORISE, Chapel Hill, NC USA. [Lin, Cynthia J.] UNC Gillings Sch Global Publ Hlth, Dept Epidemiol, Chapel Hill, NC USA. [Wade, Timothy J.; Sams, Elizabeth A.; Hilborn, Elizabeth D.] US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, 109 TW Alexander Dr,Mail Code 58A, Res Triangle Pk, NC 27709 USA. [Dufour, Alfred P.] US EPA, Microbial Chem Environm Assessment Res Div, Natl Exposure Res Lab, Off Res & Dev, Cincinnati, OH 45268 USA. [Chapman, Andrew D.] GreenWater Labs, Palatka, FL USA. RP Hilborn, ED (reprint author), US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, 109 TW Alexander Dr,Mail Code 58A, Res Triangle Pk, NC 27709 USA. EM Hilborn.E@epa.gov FU U.S. EPA FX Funding was provided by the U.S. EPA. NR 52 TC 0 Z9 0 U1 3 U2 17 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2016 VL 124 IS 4 BP 477 EP 483 DI 10.1289/ehp.1409558 PG 7 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DI9OU UT WOS:000373833100022 PM 26383636 ER PT J AU Birnbaum, LS Burke, TA Jones, JJ AF Birnbaum, Linda S. Burke, Thomas A. Jones, James J. TI Informing 21st-Century Risk Assessments with 21st-Century Science SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article AB Understanding and preventing adverse impacts from chemicals in the environment is fundamental to protecting public health, and chemical risk assessments are used to inform public health decisions in the United States and around the world. Traditional chemical risk assessments focus on health effects of environmental contaminants on a chemical-by-chemical basis, largely based on data from animal models using exposures that are typically higher than those experienced by humans. Results from environmental epidemiology studies sometimes show effects that are not observed in animal studies at human exposure levels that are lower than those used in animal studies. In addition, new approaches such as Toxicology in the 21st Century ( Tox21) and exposure forecasting ( ExpoCast) are generating mechanistic data that provide broad coverage of chemical space, chemical mixtures, and potential associated health outcomes, along with improved exposure estimates. It is becoming clear that risk assessments in the future will need to use the full range of available mechanistic, animal, and human data to integrate multiple types of data and to consider nontraditional health outcomes and end points. This perspective was developed at the "Strengthening the Scientific Basis of Chemical Safety Assessments" workshop, which was cosponsored by the U.S. Environmental Protection Agency and the National Institute of Environmental Health Sciences, where gaps between the emerging science and traditional chemical risk assessments were explored, and approaches for bridging the gaps were considered. C1 [Birnbaum, Linda S.] NIEHS, Off Director, NIH, DHHS, POB 12233, Res Triangle Pk, NC 27709 USA. [Burke, Thomas A.] US EPA, Off Res & Dev, Washington, DC 20460 USA. [Jones, James J.] US EPA, Off Chem Safety & Pollut Prevent, Washington, DC 20460 USA. RP Birnbaum, LS (reprint author), NIEHS, Off Director, NIH, DHHS, POB 12233, Res Triangle Pk, NC 27709 USA. EM birnbaumls@niehs.nih.gov NR 3 TC 0 Z9 0 U1 7 U2 18 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2016 VL 124 IS 4 BP A60 EP A63 DI 10.1289/ehp.1511135 PG 4 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DI9OU UT WOS:000373833100002 PM 27035154 ER PT J AU Kapoor, V Li, X Chandran, K Impellitteri, CA Domingo, JWS AF Kapoor, Vikram Li, Xuan Chandran, Kartik Impellitteri, Christopher A. Domingo, Jorge W. Santo TI Use of functional gene expression and respirometry to study wastewater nitrification activity after exposure to low doses of copper SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Nitrification activity; Copper exposure; RT-qPCR; sOUR; Wastewater ID AMMONIA-OXIDIZING BACTERIA; NITROSOMONAS-EUROPAEA 19718; REAL-TIME PCR; ACTIVATED-SLUDGE; HEAVY-METALS; NITRIFYING BACTERIA; TREATMENT REACTORS; NITRITE OXIDATION; MICROBIAL ECOLOGY; NITROGEN REMOVAL AB Autotrophic nitrification in biological nitrogen removal systems has been shown to be sensitive to the presence of heavy metals in wastewater treatment plants. Using transcriptase-quantitative polymerase chain reaction (RT-qPCR) data, we examined the effect of copper on the relative expression of functional genes (i.e., amoA, hao, nirK, and norB) involved in redox nitrogen transformation in batch enrichment cultures obtained from a nitrifying bioreactor operated as a continuous reactor (24-h hydraulic retention time). 16S ribosomal RNA (rRNA) gene next-generation sequencing showed that Nitrosomonas-like populations represented 60-70 % of the bacterial community, while other nitrifiers represented < 5 %. We observed a strong correspondence between the relative expression of amoA and hao and ammonia removal in the bioreactor. There were no considerable changes in the transcript levels of amoA, hao, nirK, and norB for nitrifying samples exposed to copper dosages ranging from 0.01 to 10 mg/L for a period of 12 h. Similar results were obtained when ammonia oxidation activity was measured via specific oxygen uptake rate (sOUR). The lack of nitrification inhibition by copper at doses lower than 10 mg/L may be attributed to the role of copper as cofactor for ammonia monooxygenase or to the sub-inhibitory concentrations of copper used in this study. Overall, these results demonstrate the use of molecular methods combined with conventional respirometry assays to better understand the response of wastewater nitrifying systems to the presence of copper. C1 [Kapoor, Vikram; Li, Xuan] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Kapoor, Vikram; Li, Xuan; Impellitteri, Christopher A.; Domingo, Jorge W. Santo] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. [Chandran, Kartik] Columbia Univ, Dept Earth & Environm Engn, 500 West 120th St, New York, NY 10027 USA. RP Domingo, JWS (reprint author), US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. EM santodomingo.jorge@epa.gov FU ORISE-EPA Research Fellowship; Water Environment Research Foundation; US Environmental Protection Agency, through its Office of Research and Development FX We thank Kit Daniels for building the nitrifying bioreactor and for technical assistance. VK and XL were supported by ORISE-EPA Research Fellowship. KC was supported by the Water Environment Research Foundation. The US Environmental Protection Agency, through its Office of Research and Development, funded and managed, or partially funded and collaborated in, the research described herein. This work has been subjected to the agency's administrative review and has been approved for external publication. Any opinions expressed in this paper are those of the authors and do not necessarily reflect the views of the agency; therefore, no official endorsement should be inferred. Any mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 47 TC 3 Z9 3 U1 9 U2 34 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD APR PY 2016 VL 23 IS 7 BP 6443 EP 6450 DI 10.1007/s11356-015-5843-2 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DI6TF UT WOS:000373632400042 PM 26627696 ER PT J AU Wagner, AM Larson, DL DalSoglio, JA Harris, JA Labus, P Rosi-Marshall, EJ Skrabis, KE AF Wagner, Anne M. Larson, Diane L. DalSoglio, Julie A. Harris, James A. Labus, Paul Rosi-Marshall, Emma J. Skrabis, Kristin E. TI A framework for establishing restoration goals for contaminated ecosystems SO INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT LA English DT Article DE Decision support; Remediation; Restoration; Restoration Goals ID ECOLOGY; CONSERVATION; REMEDIATION; PERSPECTIVE; SPILL; RISK AB As natural resources become increasingly limited, the value of restoring contaminated sites, both terrestrial and aquatic, becomes increasingly apparent. Traditionally, goals for remediation have been set before any consideration of goals for ecological restoration. The goals for remediation have focused on removing or limiting contamination whereas restoration goals have targeted the ultimate end use. Here, we present a framework for developing a comprehensive set of achievable goals for ecological restoration of contaminated sites to be used in concert with determining goals for remediation. This framework was developed during a Society of Environmental Toxicology and Chemistry (SETAC) and Society of Ecological Restoration (SER) cosponsored workshop that brought together experts from multiple countries. Although most members were from North America, this framework is designed for use internationally. We discuss the integration of establishing goals for both contaminant remediation and overall restoration, and the need to include both the restoration of ecological and socio-cultural-economic value in the context of contaminated sites. Although recognizing that in some countries there may be regulatory issues associated with contaminants and clean up, landscape setting and social drivers can inform the restoration goals. We provide a decision tree support tool to guide the establishment of restoration goals for contaminated ecosystems. The overall intent of this decision tree is to provide a framework for goal setting and to identify outcomes achievable given the contamination present at a site. Integr Environ Assess Manag 2016;12:264-272. 2015 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals, Inc. on behalf of Society of Environmental Toxicology & Chemistry (SETAC) C1 [Wagner, Anne M.] Chevron Energy Technol, San Ramon, CA USA. [Larson, Diane L.] US Geol Survey, St Paul, MN USA. [DalSoglio, Julie A.] US EPA, Reg 8, Helena, MT USA. [Harris, James A.] Cranfield Univ, Cranfield MK43 0AL, Beds, England. [Labus, Paul] Nature Conservancy, Merrillville, IN USA. [Rosi-Marshall, Emma J.] Cary Inst Ecosyst Studies, Millbrook, NY USA. [Skrabis, Kristin E.] US Dept Interior, Washington, DC USA. RP Wagner, AM (reprint author), Chevron Energy Technol, San Ramon, CA USA. EM awagne@chevron.com RI Harris, Jim/B-2816-2011; OI Harris, Jim/0000-0001-9266-4979; Larson, Diane/0000-0001-5202-0634 FU Anchor QEA LLC; Chevron; CSIRO; DuPont; ExxonMobil; Honeywell; Industrial Economics; Intrinsik Environmental Sciences; Integral Consulting; Matrix New World Engineering; Newmont Mining; Rio Tinto; Teck Metals; Conservation Fund; URS Corporation; US Department of the Interior; US Geological Survey-Northern Prairie Wildlife Research Center; Windward Environmental LLC FX We gratefully acknowledge the SETAC North America staff, in particular Greg Schiefer and Nikki Mayo, who provided support to the steering committee and workshop participants before, during and after the workshop. We appreciate the funding support from the following groups that made the workshop possible: Anchor QEA LLC, Chevron, CSIRO, DuPont, ExxonMobil, Honeywell, Industrial Economics, Intrinsik Environmental Sciences, Integral Consulting, Matrix New World Engineering, Newmont Mining, Rio Tinto, Teck Metals, The Conservation Fund, URS Corporation, US Department of the Interior, US Geological Survey-Northern Prairie Wildlife Research Center. and Windward Environmental LLC. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. NR 60 TC 4 Z9 4 U1 9 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1551-3777 EI 1551-3793 J9 INTEGR ENVIRON ASSES JI Integr. Environ. Assess. Manag. PD APR PY 2016 VL 12 IS 2 BP 264 EP 272 DI 10.1002/ieam.1709 PG 9 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DI3KG UT WOS:000373396900007 PM 26339869 ER PT J AU Erban, LE Gorelick, SM AF Erban, Laura E. Gorelick, Steven M. TI Closing the irrigation deficit in Cambodia: Implications for transboundary impacts on groundwater and Mekong River flow SO JOURNAL OF HYDROLOGY LA English DT Article DE Paddy; Agricultural water use; Dry season rice; Groundwater pumping ID ARSENIC POLLUTION; VIETNAM; DELTA; SYSTEMS; WATER AB Rice production in Cambodia, essential to food security and exports, is largely limited to the wet season. The vast majority (96%) of land planted with rice during the wet season remains fallow during the dry season. This is in large part due to lack of irrigation capacity, increases in which would entail significant consequences for Cambodia and Vietnam, located downstream on the Mekong River. Here we quantify the extent of the dry season "deficit" area in the Cambodian Mekong River catchment, using a recent agricultural survey and our analysis of MODIS satellite data. Irrigation of this land for rice production would require a volume of water up to 31% of dry season Mekong River flow to Vietnam. However, the two countries share an aquifer system in the Mekong Delta, where irrigation demand is increasingly met by groundwater. We estimate expansion rates of groundwater-irrigated land to be >10% per year in the Cambodian Delta using LANDSAT satellite data and simulate the effects of future expansion on groundwater levels over a 25-year period. If groundwater irrigation continues to expand at current rates, the water table will drop below the lift limit of suction pump wells, used for domestic supply by >1.5 million people, throughout much of the area within 15 years. Extensive groundwater irrigation jeopardizes access for shallow domestic water supply wells, raises the costs of pumping for all groundwater users, and may exacerbate arsenic contamination and land subsidence that are already widespread hazards in the region. Published by Elsevier B.V. C1 [Erban, Laura E.; Gorelick, Steven M.] Stanford Univ, Dept Earth Syst Sci, 473 Via Ortega, Stanford, CA 94305 USA. RP Erban, LE (reprint author), US EPA, ORD, NHEERL, Atlantic Ecol Div, 27 Tarzwell Dr, Narragansett, RI 02882 USA. EM lerban@stanford.edu; gorelick@stanford.edu FU National Science Foundation [EAR-1313518] FX We gratefully acknowledge the UPS Endowment Fund and the Global Freshwater Initiative of the Woods Institute for the Environment at Stanford. This work was supported by the National Science Foundation under grant EAR-1313518 to Stanford University. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. NR 20 TC 1 Z9 1 U1 10 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD APR PY 2016 VL 535 BP 85 EP 92 DI 10.1016/j.jhydrol.2016.01.072 PG 8 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DI3TN UT WOS:000373421500008 ER PT J AU Haugland, RA Siefring, S Varma, M Oshima, KH Sivaganesan, M Cao, YP Raith, M Griffith, J Weisberg, SB Noble, RT Blackwood, AD Kinzelman, J Anan'eva, T Bushon, RN Stelzer, EA Harwood, VJ Gordon, KV Sinigalliano, C AF Haugland, Richard A. Siefring, Shawn Varma, Manju Oshima, Kevin H. Sivaganesan, Mano Cao, Yiping Raith, Meredith Griffith, John Weisberg, Stephen B. Noble, Rachel T. Blackwood, A. Denene Kinzelman, Julie Anan'eva, Tamara Bushon, Rebecca N. Stelzer, Erin A. Harwood, Valarie J. Gordon, Katrina V. Sinigalliano, Christopher TI Multi-laboratory survey of qPCR enterococci analysis method performance in US coastal and inland surface waters SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Enterococci; qPCR; Interference; Performance; EPA Method 1609; EPA Method 1611 ID POLYMERASE-CHAIN-REACTION; CULTURE-BASED METHODS; QUANTITATIVE PCR; ENVIRONMENTAL WATERS; MARINE BEACHES; ENUMERATION; QUALITY; QUANTIFICATION; INDICATORS; ILLNESS AB Quantitative polymerase chain reaction (qPCR) has become a frequently used technique for quantifying enterococci in recreational surface waters, but there are several methodological options. Here we evaluated how three method permutations, type of mastermix, sample extract dilution and use of controls in results calculation, affect method reliability among multiple laboratories with respect to sample interference. Multiple samples from each of 22 sites representing an array of habitat types were analyzed using EPA Method 1611 and 1609 reagents with full strength and five-fold diluted extracts. The presence of interference was assessed three ways: using sample processing and PCR amplifications controls; consistency of results across extract dilutions; and relative recovery of target genes from spiked enterococci in water sample compared to control matrices with acceptable recovery defined as 50 to 200%. Method 1609, which is based on an environmental mastermix, was found to be superior to Method 1611, which is based on a universal mastermix. Method 1611 had over a 40% control assay failure rate with undiluted extracts and a 6% failure rate with diluted extracts. Method 1609 failed in only 11% and 3% of undiluted and diluted extracts analyses. Use of sample processing control assay results in the delta-delta Ct method for calculating relative target gene recoveries increased the number of acceptable recovery results. Delta-delta tended to bias recoveries from apparent partially inhibitory samples on the high side which could help in avoiding potential underestimates of enterococci - an important consideration in a public health context. Control assay and delta-delta recovery results were largely consistent across the range of habitats sampled, and among laboratories. The methodological option that best balanced acceptable estimated target gene recoveries with method sensitivity and avoidance of underestimated enterococci densities was Method 1609 without extract dilution and using the delta-delta calculation method. The applicability of this method can be extended by the analysis of diluted extracts to sites where interference is indicated but, particularly in these instances, should be confirmed by augmenting the control assays with analyses for target gene recoveries from spiked target organisms. Published by Elsevier B.V. C1 [Haugland, Richard A.; Siefring, Shawn; Varma, Manju; Oshima, Kevin H.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [Sivaganesan, Mano] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Cao, Yiping; Raith, Meredith; Griffith, John; Weisberg, Stephen B.] Southern Calif Coastal Water Res Project Author, Costa Mesa, CA USA. [Noble, Rachel T.; Blackwood, A. Denene] Univ North Carolina Chapel Hill, Inst Marine Sci, Morehead City, NC USA. [Kinzelman, Julie; Anan'eva, Tamara] City Racine Hlth Dept, Racine, WI USA. [Bushon, Rebecca N.; Stelzer, Erin A.] US Geol Survey, Columbus, OH USA. [Harwood, Valarie J.; Gordon, Katrina V.] Univ S Florida, Dept Integrat Biol, Tampa, FL USA. [Sinigalliano, Christopher] NOAA, Atlantic Oceanog & Meteorol Lab, Ocean Chem Div, Miami, FL 33149 USA. RP Haugland, RA (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. EM haugland.rich@epa.gov RI Weisberg, Stephen/B-2477-2008; Sinigalliano, Christopher/A-8760-2014 OI Weisberg, Stephen/0000-0002-0655-9425; Sinigalliano, Christopher/0000-0002-9942-238X NR 32 TC 1 Z9 1 U1 2 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD APR PY 2016 VL 123 BP 114 EP 125 DI 10.1016/j.mimet.2016.01.017 PG 12 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA DI7BY UT WOS:000373655300017 PM 26844886 ER PT J AU Tuma, MW Millington, C Schumaker, N Burnett, P AF Tuma, Michael W. Millington, Chris Schumaker, Nathan Burnett, Paul TI Modeling Agassiz's Desert Tortoise Population Response to Anthropogenic Stressors SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Agassiz's desert tortoise; anthropogenic threats; Gopherus agassizii; HexSim; population model; predictive occurrence model ID INDIVIDUAL-BASED MODEL; RESPIRATORY-TRACT DISEASE; COMMON RAVEN PREDATION; SEA-TURTLE POPULATION; CENTRAL MOJAVE DESERT; LONG-LIVED ORGANISMS; GOPHERUS-AGASSIZII; CHELYDRA-SERPENTINA; EGG-PRODUCTION; HOME-RANGE AB Agassiz's desert tortoise (Gopherus agassizii) populations are exposed to a variety of anthropogenic threats, which vary in nature, distribution, severity, and frequency. Tortoise management in conservation areas can be compromised when the relative importance of these threats is not well understood. We used HexSim to develop simulation models for desert tortoise populations occupying 2 study areas in the western-central (Superior Cronese in California, USA) and the eastern (Gold Butte-Pakoon in Nevada and Arizona, USA) Mojave Desert, each with a distinct set of site-specific threats. We developed threats models that were parameterized from published information, and conducted independent simulations of threats at varying levels of severity for each study area. Modeled tortoise populations in both study areas were subjected to simulations of threats associated with human presence and subsidized predators. Additional simulated threats in the Superior Cronese model included disease and habitat degradation on land in-holdings, whereas tortoise populations in the Gold Butte-Pakoon model were further exposed to simulations of wildfire, livestock grazing, and feral burros. We used our 2 study area-specific simulation models to rank the threats' relative importance to desert tortoise population viability. Threats more widely distributed in time and space within the modeled conservation areas significantly limited tortoise population growth more than threats that were patchily distributed or temporally dynamic. Our use of a spatially explicit population model allowed us to evaluate and prioritize the effects of threats over site-specific, dynamic, simulated landscapes, which differed from previous modeling efforts for desert tortoises. Our threat prioritization will inform and improve ongoing management efforts attempting to increase desert tortoise population viability by altering anthropogenic disturbance regimes. (C) 2016 The Wildlife Society. C1 [Tuma, Michael W.; Millington, Chris] 150 S Arroyo Pkwy,Second Floor, Pasadena, CA 91105 USA. [Schumaker, Nathan] US EPA, 200 SW 35th St, Corvallis, OR 97333 USA. [Burnett, Paul] 295 Interlocken Blvd,Suite 300, Broomfield, CO 80021 USA. [Tuma, Michael W.] Western EcoSyst Technol Inc, POB 7081, Redlands, CA 92373 USA. RP Tuma, MW (reprint author), 150 S Arroyo Pkwy,Second Floor, Pasadena, CA 91105 USA.; Tuma, MW (reprint author), Western EcoSyst Technol Inc, POB 7081, Redlands, CA 92373 USA. EM mtuma@west-inc.com FU BLM [L09PA00570]; SWCA Environmental Consultants; U.S. Environmental Protection Agency FX S. Hoddapp commissioned and directed the study, and BLM biologists and managers from the Washington Office, the National Operations Center, State Offices for Arizona, California, and Nevada, the Arizona Strip Field Office, the Barstow Field Office, and the Southern Nevada District Office provided guidance and input, including N. Moore, K. Tripp, F. Quamen, C. Ronning, L. LePre, E. Masters, A. Fesnock, M. Quillman, and K. Jensen. C. Flynn, R. Gutierrez, C. Moller, and E. Riveramelo provided GIS support, and C. Cross assisted with statistical analyses. This study was subjected to review by the National Health and Environmental Effects Research Laboratory's Western Ecology Division and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. The HexSim population and threats models that supported this study were submitted to the BLM National Office, BLM State Offices in Arizona, California, and Nevada, BLM District Offices for California Desert and Southern Nevada, and the Arizona Strip Field Office. This study was funded by the BLM under a contract (L09PA00570) with SWCA Environmental Consultants and in part by the U.S. Environmental Protection Agency. NR 98 TC 0 Z9 0 U1 14 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD APR PY 2016 VL 80 IS 3 BP 414 EP 429 DI 10.1002/jwmg.1044 PG 16 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DI4OV UT WOS:000373480100004 ER PT J AU Wu, QS Lane, CR AF Wu, Qiusheng Lane, Charles R. TI Delineation and Quantification of Wetland Depressions in the Prairie Pothole Region of North Dakota SO WETLANDS LA English DT Article DE Wetland hydrology; Topographic depressions; Water storage; LiDAR; Prairie Pothole Region; Geographically isolated wetland; Non-adjacent wetland ID DIGITAL ELEVATION MODELS; COTTONWOOD LAKE AREA; QUANTITATIVE DESCRIPTION; TOPOGRAPHIC DEPRESSIONS; HYDROLOGIC CONNECTIVITY; CONCEPTUAL-FRAMEWORK; SURFACE DEPRESSIONS; WATER STORAGE; LIDAR DATA; INUNDATION AB The Prairie Pothole Region of North America is characterized by numerous, small, wetland depressions that perform important ecological and hydrological functions. Recent studies have shown that total wetland area in the region is decreasing due to cumulative impacts related to natural and anthropogenic changes. The impact of wetland losses on landscape hydrology is an active area of research and management. Various spatially distributed hydrologic models have been developed to simulate effects of wetland depression storage on peak river flows, frequently using dated geospatial wetland inventories. We describe an innovative method for identifying wetland depressions and quantifying their nested hierarchical bathymetric/topographic structure using high-resolution light detection and ranging (LiDAR) data. This contour tree method allows identified wetland depressions to be quantified based on their dynamic filling-spilling-merging hydrological processes. In addition, wetland depression properties, such as surface area, maximum depth, mean depth, storage volume, etc., can be computed for each component of a depression as well as the compound depression. We successfully applied the proposed method to map wetland depressions in the Little Pipestem Creek watershed in North Dakota. The methods described in this study will provide more realistic and higher resolution data layers for hydrologic modeling and other studies requiring characterization of simple and complex wetland depressions, and help prioritize conservation planning efforts for wetland resources. C1 [Wu, Qiusheng] SUNY Binghamton, Dept Geog, Binghamton, NY 13902 USA. [Lane, Charles R.] US EPA, Off Res & Dev, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Wu, Qiusheng] US EPA, CSS Dynamac, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. RP Wu, QS (reprint author), SUNY Binghamton, Dept Geog, Binghamton, NY 13902 USA.; Wu, QS (reprint author), US EPA, CSS Dynamac, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM wqs@binghamton.edu; Lane.Charles@epa.gov OI Wu, Qiusheng/0000-0001-5437-4073 NR 44 TC 2 Z9 2 U1 8 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 EI 1943-6246 J9 WETLANDS JI Wetlands PD APR PY 2016 VL 36 IS 2 BP 215 EP 227 DI 10.1007/s13157-015-0731-6 PG 13 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DI6XQ UT WOS:000373643900002 ER PT J AU Berninger, JP LaLone, CA Villeneuve, DL Ankley, GT AF Berninger, Jason P. LaLone, Carlie A. Villeneuve, Daniel L. Ankley, Gerald T. TI PRIORITIZATION OF PHARMACEUTICALS FOR POTENTIAL ENVIRONMENTAL HAZARD THROUGH LEVERAGING A LARGE-SCALE MAMMALIAN PHARMACOLOGICAL DATASET SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Pharmaceuticals; Risk ranking; Hazard/risk assessment ID PERSONAL CARE PRODUCTS; RISK-ASSESSMENT; VETERINARY MEDICINES; AQUATIC ORGANISMS; FISH; ECOTOXICOLOGY; SUBSTANCES; RANKING; ANTIDEPRESSANTS; EXTRAPOLATION AB The potential for pharmaceuticals in the environment to cause adverse ecological effects is of increasing concern. Given the thousands of active pharmaceutical ingredients (APIs) that can enter the aquatic environment through human and/or animal (e.g., livestock) waste, a current challenge in aquatic toxicology is identifying those that pose the greatest risk. Because empirical toxicity information for aquatic species is generally lacking for pharmaceuticals, an important data source for prioritization is that generated during the mammalian drug development process. Applying concepts of species read-across, mammalian pharmacokinetic data were used to systematically prioritize APIs by estimating their potential to cause adverse biological consequences to aquatic organisms, using fish as an example. Mammalian absorption, distribution, metabolism, and excretion (ADME) data (e.g., peak plasma concentration, apparent volume of distribution, clearance rate, and half-life) were collected and curated, creating the Mammalian Pharmacokinetic Prioritization For Aquatic Species Targeting (MaPPFAST) database representing 1070 APIs. From these data, a probabilistic model and scoring system were developed and evaluated. Individual APIs and therapeutic classes were ranked based on clearly defined read-across assumptions for translating mammalian-derived ADME parameters to estimate potential hazard in fish (i.e., greatest predicted hazard associated with lowest mammalian peak plasma concentrations, total clearance and highest volume of distribution, half-life). It is anticipated that the MaPPFAST database and the associated API prioritization approach will help guide research and/or inform ecological risk assessment. Published 2015 Wiley Periodicals Inc. on behalf of SETAC. This article is a US Government work and, as such, is in the public domain in the United States of America. C1 [Berninger, Jason P.] US EPA, Natl Res Council, Duluth, MN USA. [LaLone, Carlie A.] Univ Minnesota, Water Resources Ctr, Coll Food Agr & Nat Resource Sci, St Paul, MN 55108 USA. [LaLone, Carlie A.; Villeneuve, Daniel L.; Ankley, Gerald T.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Midcontinent Ecol Div, Duluth, MN USA. RP Berninger, JP (reprint author), US EPA, Natl Res Council, Duluth, MN USA. EM JasonBerninger@gmail.com RI Berninger, Jason/O-2401-2016 OI Berninger, Jason/0000-0003-3045-7899 NR 62 TC 3 Z9 3 U1 8 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD APR PY 2016 VL 35 IS 4 BP 1007 EP 1020 DI 10.1002/etc.2965 PG 14 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DH0RB UT WOS:000372490300027 PM 25772004 ER PT J AU Eastwood, G Loaiza, JR Pongsiri, MJ Sanjur, OI Pecor, JE Auguste, AJ Kramer, LD AF Eastwood, Gillian Loaiza, Jose R. Pongsiri, Montira J. Sanjur, Oris I. Pecor, James E. Auguste, Albert J. Kramer, Laura D. TI Enzootic Arbovirus Surveillance in Forest Habitat and Phylogenetic Characterization of Novel Isolates of Gamboa Virus in Panama SO AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE LA English DT Article ID VENEZUELAN EQUINE ENCEPHALITIS; AEDEOMYIA-SQUAMIPENNIS; YELLOW-FEVER; MOSQUITOS DIPTERA; CULICIDAE; AMERICA; IDENTIFICATION; BUNYAVIRUSES; TRANSMISSION; DIVERSITY AB Landscape changes occurring in Panama, a country whose geographic location and climate have historically supported arbovirus transmission, prompted the hypothesis that arbovirus prevalence increases with degradation of tropical forest habitats. Investigations at four variably degraded sites revealed a diverse array of potential mosquito vectors, several of which are known vectors of arbovirus pathogens. Overall, 675 pools consisting of 25,787 mosquitoes and representing 29 species from nine genera (collected at ground and canopy height across all habitats) were screened for cytopathic viruses on Vero cells. We detected four isolates of Gamboa virus (family: Bunyaviridae; genus: Ortho-bunyavirus) from pools of Aedeomyia squamipennis captured at canopy level in November 2012. Phylogenetic characterization of complete genome sequences shows the new isolates to be closely related to each other with strong evidence of reassortment among the M segment of Panamanian Gamboa isolates and several other viruses of this group. At the site yielding viruses, Soberania National Park in central Panama, 18 mosquito species were identified, and the predominant taxa included A. squamipennis, Coquillettidia nigricans, and Mansonia titillans. C1 [Eastwood, Gillian; Kramer, Laura D.] New York State Dept Hlth, Wadsworth Ctr, Slingerlands, NY USA. [Loaiza, Jose R.] Inst Invest Cient & Serv Alta Tecnol INDICASAT, Ciudad Del Saber, Panama. [Pongsiri, Montira J.] US EPA, Washington, DC 20460 USA. Smithsonian Trop Res Inst, Panama City, Panama. Walter Reed Biosystemat Unit, Suitland, MD USA. [Auguste, Albert J.] Univ Texas Med Branch, Dept Pathol, Keiller Bldg, Galveston, TX 77555 USA. [Sanjur, Oris I.] Smithsonian Trop Res Inst, Mol Biol & Evolut Labs, Ancon, Panama. [Pecor, James E.] Walter Reed Army Med Ctr, Walter Reed Army Inst Res, Dept Entomol, Washington, DC 20307 USA. RP Eastwood, G (reprint author), Univ Texas Med Branch, Dept Pathol, Keiller Bldg, Galveston, TX 77555 USA. EM gieastwo@utmb.edu; jloaiza@indicasat.org.pa; pongsiri.montira@epa.gov; sanjuro@si.edu; pecorj@si.edu; ajlaugus@utmb.edu; laura.kramer@health.ny.gov FU Robert E. Shope International Fellowship Award - American Society of Tropical Medicine and Hygiene; James W. McLaughlin Endowment fund; National Research Investigator Board (SNI) of the Secretariat for Science, Technology and Innovation of Panama (SENACYT); U.S. Environmental Protection Agency [DW33-92296801-0] FX Funding for this project was provided via the Robert E. Shope International Fellowship Award granted by the American Society of Tropical Medicine and Hygiene to Gillian Eastwood in 2012. Gillian Eastwood and Albert J. Auguste are also funded by the James W. McLaughlin Endowment fund. Jose R. Loaiza is funded by the National Research Investigator Board (SNI) of the Secretariat for Science, Technology and Innovation of Panama (SENACYT). The U.S. Environmental Protection Agency project "Mosquito Species Diversity and Landscape Change DW33-92296801-0" awarded to Montira Pongsiri covered the costs of mosquito sampling at Las Pavas, Achiote, and Barro Colorado Island, and some of the laboratory supplies and reagents used at NYSDOH. NR 45 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC TROP MED & HYGIENE PI MCLEAN PA 8000 WESTPARK DR, STE 130, MCLEAN, VA 22101 USA SN 0002-9637 EI 1476-1645 J9 AM J TROP MED HYG JI Am. J. Trop. Med. Hyg. PD APR PY 2016 VL 94 IS 4 BP 786 EP 793 DI 10.4269/ajtmh.15-0445 PG 8 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA DI1FQ UT WOS:000373242400015 PM 26834200 ER PT J AU Kelly, JT Baker, KR Nolte, CG Napelenok, SL Keene, WC Pszenny, AAP AF Kelly, James T. Baker, Kirk R. Nolte, Christopher G. Napelenok, Sergey L. Keene, William C. Pszenny, Alexander A. P. TI Simulating the phase partitioning of NH3, HNO3, and HCl with size-resolved particles over northern Colorado in winter SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Aerosol size distributions; Source apportionment; NACHTT ID AIR-QUALITY MODEL; THERMODYNAMIC-EQUILIBRIUM MODEL; SAN-JOAQUIN VALLEY; PARTICULATE MATTER; UNITED-STATES; SENSITIVITY-ANALYSIS; CMAQ MODEL; EMISSIONS INVENTORY; CALIFORNIA; AEROSOLS AB Numerical modeling of inorganic aerosol processes is useful in air quality management, but comprehensive evaluation of modeled aerosol processes is rarely possible due to the lack of comprehensive datasets. During the Nitrogen, Aerosol Composition, and Halogens on a Tall Tower (NACHTT) campaign in February and March 2011, the phase partitioning of soluble trace gases with size-resolved particles and related meteorological conditions were measured continuously at a site in Colorado about 33 km north of Denver. These size-resolved measurements of particulate SO42-, NH4+, NO3-, Cl-, Na+, Ca2+, Mg2+, and K+ and inorganic gases are used here to assess the ability of a continental U.S. modeling platform to simulate the gas-particle partitioning of NH3, HNO3, and HCl at this location. Modeling is based on the Community Multiscale Air Quality (CMAQ) model with 12 km horizontal resolution. Baseline, sensitivity, and source apportionment simulations are conducted to fully characterize the model predictions. Considering the limitations in representing regional terrain features in the national 12 km modeling, predictions agree reasonably well with measured concentrations and gas-particle partitioning at this location. However, the median sum of NH4+ and NH3 is underpredicted in the baseline simulation by a factor of four suggesting a need for improved bottom-up NH3 emissions inventories in this area. The median sum of Cl- and HCI is underpredicted by a factor of 2.8, while levels of Ca2+, Mg2+, and K+ are overpredicted. Improvements in windblown and fugitive dust emissions may improve and/or better constrain these predictions. Mass size distributions for inorganic particle constituents are generally simulated well, although the modeled fine particle mode is shifted to slightly larger diameters relative to measurements. Source apportionment modeling estimates of source sector and boundary contributions to air quality at the site are provided. Published by Elsevier Ltd. C1 [Kelly, James T.; Baker, Kirk R.] US EPA, Off Air Qual Planning & Stand, Res Triangle Pk, NC 27711 USA. [Nolte, Christopher G.; Napelenok, Sergey L.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Keene, William C.] Univ Virginia, Dept Environm Sci, Clark Hall, Charlottesville, VA 22903 USA. [Pszenny, Alexander A. P.] Univ New Hampshire, Inst Study Earth Oceans & Space, Durham, NH 03824 USA. RP Kelly, JT (reprint author), US EPA, Air Qual Modeling Grp, Air Qual Assessment Div, Off Air Qual Planning & Stand,Off Air & Radiat, 109 TW Alexander Dr,Mail Drop C439-01, Res Triangle Pk, NC 27711 USA. EM kelly.james@epa.gov RI Nolte, Christopher/H-4345-2012; Kelly, James/F-8135-2010 OI Nolte, Christopher/0000-0001-5224-9965; Kelly, James/0000-0001-6574-5714 FU National Science Foundation [ANT-1041187, ANT-1041049] FX We acknowledge the use of the Boulder Atmospheric Observatory (BAO), and Daniel Wolfe and Bruce Bartram of the NOAA/ESRL Physical Sciences Division, and William Dube and Nicholas Wagner of NOAA/ESRL Chemical Sciences Division and CIRES, for their help in conducting the measurements at the BAO. The authors also thank the U.S. EPA Modeling Platform Development Team and Allan Beidler, James Beidler, Chris Allen, and Lara Reynolds for contributions to the modeling platform. We thank Dr. Daniel Bon of CDPHE for providing information on animal units. Chemical sampling and analysis were supported by National Science Foundation awards ANT-1041187 to the University of Virginia and ANT-1041049 to the University of New Hampshire. NR 46 TC 1 Z9 1 U1 7 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD APR PY 2016 VL 131 BP 67 EP 77 DI 10.1016/j.atmosenv.2016.01.049 PG 11 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DH4NT UT WOS:000372763400007 ER PT J AU Liu, XY Guo, ZS Krebs, KA Greenwell, DJ Roache, NF Stinson, RA Nardin, JA Pope, RH AF Liu, Xiaoyu Guo, Zhishi Krebs, Kenneth A. Greenwell, Dale J. Roache, Nancy F. Stinson, Rayford A. Nardin, Joshua A. Pope, Robert H. TI Laboratory study of PCB transport from primary sources to settled dust SO CHEMOSPHERE LA English DT Article DE Polychlorinated biphenyls (PCBs); Settled dust; Chamber testing; Dust/air partition; Dust/source partition ID DIOXIN-LIKE PCBS; IN-HOUSE DUST; POLYCHLORINATED-BIPHENYLS PCBS; DAY-CARE-CENTERS; INDOOR DUST; EXPOSURE; AIR; BUILDINGS; CHEMICALS; SCHOOL AB Dust is an important sink for indoor air pollutants, such as polychlorinated biphenyls (PCBs) that were used in building materials and products. In this study, two types of dust, house dust and Arizona Test Dust, were tested in a (3)0-m3 stainless steel chamber with two types of panels. The PCB-containing panels were aluminum sheets coated with a PCB-spiked primer or caulk. The PCB-free panels were coated with the same materials but without PCBs. The dust evenly spread on each panel was collected at different times to determine its PCB content. The data from the PCB panels were used to evaluate the PCB migration from the source to the dust through direct contact, and the data from the PCB-free panels were used to evaluate the sorption of PCBs through the dust/air partition. Settled dust can adsorb PCBs from air. The sorption concentration was dependent on the congener concentration in the air and favored less volatile congeners. When the house dust was in direct contact with the PCB-containing panel, PCBs migrated into the dust at a much faster rate than the PCB transfer rate due to the dust/air partition. The dust/source partition was not significantly affected by the congener's volatility. For a given congener, the ratio between its concentration in the dust and in the source was used to estimate the dust/source partition coefficient. The estimated values ranged from 0.04 to 0.16. These values are indicative of the sink strength of the tested house dust being in the middle or lower-middle range. Published by Elsevier Ltd. C1 [Liu, Xiaoyu; Guo, Zhishi; Krebs, Kenneth A.; Greenwell, Dale J.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Air Pollut Prevent & Control Div, Res Triangle Pk, NC 27711 USA. [Roache, Nancy F.; Stinson, Rayford A.; Nardin, Joshua A.; Pope, Robert H.] US Inc, ARCADIS, 4915 Prospectus Dr,Suite F, Durham, NC 27709 USA. RP Liu, XY (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, Air Pollut Prevent & Control Div, Res Triangle Pk, NC 27711 USA. EM liu.xiaoyu@epa.gov NR 35 TC 1 Z9 1 U1 12 U2 25 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD APR PY 2016 VL 149 BP 62 EP 69 DI 10.1016/j.chemosphere.2016.01.075 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DH4MM UT WOS:000372760100009 PM 26849196 ER PT J AU Haam, JH Kim, YS Koo, HS Haam, J Seo, NK Kim, HY Park, KC Park, KS Kim, MJ AF Haam, Ji-Hee Kim, Young-Sang Koo, Hyung Suk Haam, Juhee Seo, Nam Kyoung Kim, Hyung Yuk Park, Kyung-Chae Park, Kye-Seon Kim, Moon Jong TI Intermuscular adipose tissue is associated with monocyte chemoattractant protein-1, independent of visceral adipose tissue SO CLINICAL BIOCHEMISTRY LA English DT Article DE Obesity; MCP-1; Adiponectin; Leptin; Intermuscular adipose tissue ID BODY-FAT DISTRIBUTION; INSULIN SENSITIVITY; CARDIOVASCULAR-DISEASE; LEPTIN; OBESITY; INFLAMMATION; ADIPONECTIN; RESISTANCE; GLUCOSE; WOMEN AB Objectives: Emerging evidence suggests that intermuscular adipose tissue is a risk factor for insulin resistance, but the underlying mechanism still remains unclear. We investigated whether the levels of leptin, adiponectin, and monocyte chemoattractant protein-1 are associated with intermuscular adipose tissue in obese subjects. Design and methods: A cross-sectional study was performed on 77 obese Korean women. Areas of visceral adipose tissue, subcutaneous adipose tissue, and intermuscular adipose tissue were measured by computed tomography scan, and serum concentrations of adipokines were measured by enzyme-linked immunosorbent assays. Correlation between the levels of adipokines and the fat areas was assessed using Pearson correlation and covariate-adjusted multivariable regression. Results: Leptin was positively correlated with subcutaneous adipose tissue (r = 0.452, P < 0.001), fasting insulin (r = 0.403, P < 0.001), and homeostasis model assessment of insulin resistance (r = 0.360, P = 0.001), whereas monocyte chemoattractant protein-1 was positively correlated with intermuscular adipose tissue (r = 0.483, P < 0.001). After adjustment for age, height, and other body composition metrics, leptin was still related to subcutaneous adipose tissue (beta = 0.390, P = 0.001). Monocyte chemoattractant protein-1 was associated with intermuscular adipose tissue (beta = 0.433, P = 0.001) after adjustment for visceral adipose tissue. Conclusions: Intermuscular adipose tissue was correlated with monocyte chemoattractant protein-1, suggesting its role in the development of insulin resistance. (C) 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. C1 [Haam, Ji-Hee; Kim, Young-Sang; Seo, Nam Kyoung; Kim, Hyung Yuk; Park, Kyung-Chae; Park, Kye-Seon; Kim, Moon Jong] CHA Univ, CHA Bundang Med Ctr, Dept Family Med, 59 Yatap Ro, Songnam, Gyeonggi Do, South Korea. [Koo, Hyung Suk] Dongguk Univ, Bundang Oriental Hosp, Dept Family Med, 268 Buljeong Ro, Songnam, Gyeonggi Do, South Korea. [Haam, Juhee] Natl Inst Environm Hlth Sci, Neurobiol Lab, NIH, 111 TW Alexander Dr, Durham, NC 27709 USA. RP Kim, MJ (reprint author), CHA Univ, CHA Bundang Med Ctr, Dept Family Med, 59 Yatap Ro, Songnam, Gyeonggi Do, South Korea. EM mjkimfm@cha.ac.kr FU National Institutes of Health (NIH); Daesang Corporation (Seoul, Korea); Ministry of Science, ICT and Future Planning [NRF 2013M3A9C4078153]; Ministry of Education, Science and Technology [BK21PLUS 22A20130012143] FX We are grateful to the participants in this study. This work was supported by the National Institutes of Health (NIH) intramural research program. This study was supported by Daesang Corporation (Seoul, Korea), the Ministry of Science, ICT and Future Planning (NRF 2013M3A9C4078153), and the Ministry of Education, Science and Technology (BK21PLUS 22A20130012143). The funding sources had no involvement in the design, collection, analysis, and interpretation of the data; the writing of this report; or the decision to submit this manuscript for publication. NR 44 TC 1 Z9 1 U1 0 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0009-9120 EI 1873-2933 J9 CLIN BIOCHEM JI Clin. Biochem. PD APR PY 2016 VL 49 IS 6 BP 439 EP 443 DI 10.1016/j.clinbiochem.2015.12.003 PG 5 WC Medical Laboratory Technology SC Medical Laboratory Technology GA DI0TI UT WOS:000373209700004 PM 26705742 ER PT J AU McCuen, RH Govindaraju, RS Hantush, MM AF McCuen, Richard H. Govindaraju, R. S. Hantush, M. M. TI The Art and Science of Effective Paper Reviewing SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Editorial Material C1 [McCuen, Richard H.] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Govindaraju, R. S.] Purdue Univ, Lyles Sch Civil Engn, 550 Stadium Mall Dr, W Lafayette, IN 47907 USA. [Hantush, M. M.] US EPA, Subsurface Hydrol, Cincinnati, OH 45268 USA. RP McCuen, RH (reprint author), Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. EM rhmccuen@umd.edu NR 0 TC 0 Z9 0 U1 4 U2 4 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD APR PY 2016 VL 21 IS 4 AR 01816001 DI 10.1061/(ASCE)HE.1943-5584.0001363 PG 2 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DH5CY UT WOS:000372804000015 ER PT J AU Holder, CT Cleland, JC Leduc, SD Andereck, Z Hogan, C Martin, KM AF Holder, Christopher T. Cleland, Joshua C. LeDuc, Stephen D. Andereck, Zac Hogan, Chris Martin, Kristen M. TI Generating a geospatial database of US regional feedstock production for use in evaluating the environmental footprint of biofuels SO JOURNAL OF THE AIR & WASTE MANAGEMENT ASSOCIATION LA English DT Article ID CARBON DEBT AB The potential environmental effects of increased U.S. biofuel production often vary depending upon the location and type of land used to produce biofuel feedstocks. However, complete, annual data are generally lacking regarding feedstock production by specific location. Corn is the dominant biofuel feedstock in the U.S., so here we present methods for estimating where bioethanol corn feedstock is grown annually and how much is used by U.S. ethanol biorefineries. We use geospatial software and publicly available data to map locations of biorefineries, estimate their corn feedstock requirements, and estimate the feedstock production locations and quantities. We combined these data and estimates into a Bioethanol Feedstock Geospatial Database (BFGD) for years 2005-2010. We evaluated the performance of the methods by assessing how well the feedstock geospatial model matched our estimates of locally-sourced feedstock demand. On average, the model met approximately 89 percent of the total estimated local feedstock demand across the studied years-within approximately 25-to40 kilometers of the biorefinery in the majority of cases. We anticipate that these methods could be used for other years and feedstocks, and can be subsequently applied to estimate the environmental footprint of feedstock production. Implications: Methods used to develop the Bioethanol Feedstock Geospatial Database (BFGD) provide a means of estimating the amount and location of U.S. corn harvested for use as U.S. bioethanol feedstock. Such estimates of geospatial feedstock production may be used to evaluate environmental impacts of bioethanol production and to identify conservation priorities. The BFGD is available for 2005-2010, and the methods may be applied to additional years, locations, and potentially other biofuels and feedstocks. C1 [Holder, Christopher T.] ICF Int, 2635 Meridian Pkwy,Suite 200, Durham, NC 27713 USA. [Cleland, Joshua C.] ICF Int, Saunderstown, RI USA. [LeDuc, Stephen D.] US EPA, Natl Ctr Environm Assessment, Arlington, VA USA. [Andereck, Zac] FAS Analyt, Arlington, VA USA. [Hogan, Chris] Fearless Solut LLC, Baltimore, MD USA. [Martin, Kristen M.] Montana Dept Environm Qual, Helena, MT USA. RP Holder, CT (reprint author), ICF Int, 2635 Meridian Pkwy,Suite 200, Durham, NC 27713 USA. EM chris.holder@icfi.com NR 19 TC 0 Z9 0 U1 8 U2 9 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1096-2247 EI 2162-2906 J9 J AIR WASTE MANAGE JI J. Air Waste Manage. Assoc. PD APR PY 2016 VL 66 IS 4 BP 356 EP 365 DI 10.1080/10962247.2015.1133460 PG 10 WC Engineering, Environmental; Environmental Sciences; Meteorology & Atmospheric Sciences SC Engineering; Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DH9HC UT WOS:000373106000002 PM 26727486 ER PT J AU Eisele, AP Mukerjee, S Smith, LA Thoma, ED Whitaker, DA Oliver, KD Wu, T Colon, M Alston, L Cousett, TA Miller, MC Smith, DM Stallings, C AF Eisele, Adam P. Mukerjee, Shaibal Smith, Luther A. Thoma, Eben D. Whitaker, Donald A. Oliver, Karen D. Wu, Tai Colon, Maribel Alston, Lillian Cousett, Tamira A. Miller, Michael C. Smith, Donald M. Stallings, Casson TI Volatile organic compounds at two oil and natural gas production well pads in Colorado and Texas using passive samplers SO JOURNAL OF THE AIR & WASTE MANAGEMENT ASSOCIATION LA English DT Article ID AIR-QUALITY; METHANE EMISSIONS; BARNETT SHALE; OPERATIONS AB A pilot study was conducted in application of the U.S. Environmental Protection Agency (EPA) Methods 325A/B variant for monitoring volatile organic compounds (VOCs) near two oil and natural gas (ONG) production well pads in the Texas Barnett Shale formation and Colorado Denver-Julesburg Basin (DJB), along with a traffic-dominated site in downtown Denver, CO. As indicated in the EPA method, VOC concentrations were measured for 14-day sampling periods using passive-diffusive tube samplers with Carbopack X sorbent at fenceline perimeter and other locations. VOCs were significantly higher at the DJB well pad versus the Barnett well pad and were likely due to higher production levels at the DJB well pad during the study. Benzene and toluene were significantly higher at the DJB well pad versus downtown Denver. Except for perchloroethylene, VOCs measured at passive sampler locations (PSs) along the perimeter of the Barnett well pad were significantly higher than PSs farther away. At the DJB well pad, most VOC concentrations, except perchloroethylene, were significantly higher prior to operational changes than after these changes were made. Though limited, the results suggest passive samplers are precise (duplicate precision usually <= 10%) and that they can be useful to assess spatial gradients and operational conditions at well pad locations over time-integrated periods. Implications: Recently enacted EPA Methods 325A/B use passive-diffusive tube samplers to measure benzene at multiple fenceline locations at petrochemical refineries. This pilot study presents initial data demonstrating the utility of Methods 325A/B for monitoring at ONG facilities. Measurements revealed elevated concentrations reflective of production levels and spatial gradients of VOCs relative to source proximity at the Barnett well pad, as well as operational changes at the DJB well pad. Though limited, these findings indicate that Methods 325A/B can be useful in application to characterize VOCs at well pad boundaries. C1 [Eisele, Adam P.] US EPA, Reg 8, Denver, CO USA. [Mukerjee, Shaibal; Whitaker, Donald A.; Oliver, Karen D.; Colon, Maribel; Alston, Lillian] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Smith, Luther A.; Cousett, Tamira A.; Stallings, Casson] Alion Sci & Technol, Durham, NC USA. [Thoma, Eben D.; Wu, Tai] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Alston, Lillian] Sr Environm Employment Program, Res Triangle Pk, NC USA. [Miller, Michael C.; Smith, Donald M.] US EPA, Reg 6, Dallas, TX USA. RP Mukerjee, S (reprint author), US EPA, E205-03, Res Triangle Pk, NC 27711 USA. EM Mukerjee.Shaibal@epa.gov FU U.S. Environmental Protection Agency through its Office of Research and Development [EP-D-10-070, EP-D-11-006] FX The U.S. Environmental Protection Agency through its Office of Research and Development funded and managed the research described here under contracts EP-D-10-070 and EP-D-11-006 to Alion Science and Technology and Eastern Research Group, respectively. The paper has been subjected to agency review and approved for publication. The views expressed in this paper are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. Mention of trade names or commercial products does not constitute an endorsement or recommendation for use. NR 25 TC 3 Z9 3 U1 9 U2 23 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1096-2247 EI 2162-2906 J9 J AIR WASTE MANAGE JI J. Air Waste Manage. Assoc. PD APR PY 2016 VL 66 IS 4 BP 412 EP 419 DI 10.1080/10962247.2016.1141808 PG 8 WC Engineering, Environmental; Environmental Sciences; Meteorology & Atmospheric Sciences SC Engineering; Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DH9HC UT WOS:000373106000007 PM 26771215 ER PT J AU Nelson, KR Schroeder, AL Ankley, GT Blackwell, BR Blanksma, C Degitz, SJ Flynn, KM Jensen, KM Johnson, RD Kahl, MD Knapen, D Kosian, PA Milsk, RY Randolph, EC Saari, T Stinckens, E Vergauwen, L Villeneuve, DL AF Nelson, Krysta R. Schroeder, Anthony L. Ankley, Gerald T. Blackwell, Brett R. Blanksma, Chad Degitz, Sigmund J. Flynn, Kevin M. Jensen, Kathleen M. Johnson, Rodney D. Kahl, Michael D. Knapen, Dries Kosian, Patricia A. Milsk, Rebecca Y. Randolph, Eric C. Saari, Travis Stinckens, Evelyn Vergauwen, Lucia Villeneuve, Daniel L. TI Impaired anterior swim bladder inflation following exposure to the thyroid peroxidase inhibitor 2-mercaptobenzothiazole part I: Fathead minnow SO AQUATIC TOXICOLOGY LA English DT Article DE Adverse outcome pathway; Cyprinid; Endocrine disruption; Swim bladder; Fish early life stage ID ADVERSE OUTCOME PATHWAYS; EARLY FISH DEVELOPMENT; PIMEPHALES-PROMELAS; XENOPUS-LAEVIS; SWIMBLADDER INFLATION; CONCEPTUAL-FRAMEWORK; DANIO-RERIO; END-POINTS; ZEBRAFISH; HORMONES AB In the present study, a hypothesized adverse outcome pathway linking inhibition of thyroid peroxidase (TPO) activity to impaired swim bladder inflation was investigated in two experiments in which fathead minnows (Pimephales promelas) were exposed to 2-mercaptobenzothiazole (MBT). Continuous exposure to 1 mg MBT/L for up to 22 days had no effect on inflation of the posterior chamber of the swim bladder, which typically inflates around 6 days post fertilization (dpf), a period during which maternally-derived thyroid hormone is presumed to be present. In contrast, inflation of the anterior swim bladder, which occurs around 14 dpf, was impacted. Specifically, at 14 dpf, approximately 50% of fish exposed to 1 mg MBT/L did not have an inflated anterior swim bladder. In fish exposed to MBT through 21 or 22 dpf, the anterior swim bladder was able to inflate, but the ratio of the anterior/posterior chamber length was significantly reduced compared to controls. Both abundance of thyroid peroxidase mRNA and thyroid follicle histology suggest that fathead minnows mounted a compensatory response to the presumed inhibition of TPO activity by MBT. Time-course characterization showed that fish exposed to MBT for at least 4 days prior to normal anterior swim bladder inflation had significant reductions in anterior swim bladder size, relative to the posterior chamber, compared to controls. These results, along with similar results observed in zebrafish (see part II, this issue) are consistent with the hypothesis that thyroid hormone signaling plays a significant role in mediating anterior swim bladder inflation and development in cyprinids, and that role can be disrupted by exposure to thyroid hormone synthesis inhibitors. Nonetheless, possible thyroid independent actions of MBT on anterior swim bladder inflation cannot be ruled out based on the present results. Overall, although anterior swim bladder inflation has not been directly linked to survival as posterior swim bladder inflation has, potential links to adverse ecological outcomes are plausible given involvement of the anterior chamber in sound production and detection. (C) 2016 Elsevier B.V. All rights reserved. C1 [Nelson, Krysta R.; Schroeder, Anthony L.; Ankley, Gerald T.; Degitz, Sigmund J.; Flynn, Kevin M.; Jensen, Kathleen M.; Johnson, Rodney D.; Kahl, Michael D.; Kosian, Patricia A.; Randolph, Eric C.; Saari, Travis; Villeneuve, Daniel L.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Mid Continent Ecol Div, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Schroeder, Anthony L.] Univ Minnesota Twin Cities, Water Resources Ctr, 1985 Lower Buford Circle, St Paul, MN 55108 USA. [Blackwell, Brett R.; Milsk, Rebecca Y.] US EPA, ORISE, Res Participat Program,Mid Continent Ecol Div, Off Res & Dev,Natl Hlth & Environm Effects Res La, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Blanksma, Chad] US EPA, Badger Tech Serv, Off Res & Dev, Natl Hlth & Environm Effects Res Lab,Mid Continen, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Knapen, Dries; Stinckens, Evelyn; Vergauwen, Lucia] Univ Antwerp, Dept Vet Sci, Zebrafishlab Vet Physiol & Biochem, Univ Pl 1, B-2610 Antwerp, Belgium. RP Schroeder, AL (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Mid Continent Ecol Div, 6201 Congdon Blvd, Duluth, MN 55804 USA. EM aschroed@crk.umn.edu OI Knapen, Dries/0000-0003-0472-8114 NR 39 TC 1 Z9 1 U1 7 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-445X EI 1879-1514 J9 AQUAT TOXICOL JI Aquat. Toxicol. PD APR PY 2016 VL 173 BP 192 EP 203 DI 10.1016/j.aquatox.2015.12.024 PG 12 WC Marine & Freshwater Biology; Toxicology SC Marine & Freshwater Biology; Toxicology GA DH3MA UT WOS:000372689900019 PM 26852267 ER PT J AU Stinckens, E Vergauwen, L Schroeder, AL Maho, W Blackwell, BR Witters, H Blust, R Ankley, GT Covaci, A Villeneuve, DL Knapen, D AF Stinckens, Evelyn Vergauwen, Lucia Schroeder, Anthony L. Maho, Walid Blackwell, Brett R. Witters, Hilda Blust, Ronny Ankley, Gerald T. Covaci, Adrian Villeneuve, Daniel L. Knapen, Dries TI Impaired anterior swim bladder inflation following exposure to the thyroid peroxidase inhibitor 2-mercaptobenzothiazole part II: Zebrafish SO AQUATIC TOXICOLOGY LA English DT Article DE Thyroid disruption; 2-Mercaptobenzothiazole; Zebrafish embryo; Swim bladder inflation; Fish early-life stage; Adverse outcome pathway ID ADVERSE OUTCOME PATHWAYS; DANIO-RERIO; THYROPEROXIDASE INHIBITION; IODOTHYRONINE DEIODINASES; EMBRYONIC-DEVELOPMENT; LARVAL DEVELOPMENT; WASTE-WATER; HORMONE; SWIMBLADDER; BENZOTHIAZOLES AB Disruption of the thyroid hormone (TH) system, an important mode of action, can lead to ecologically relevant adverse outcomes, especially during embryonic development. The present study characterizes the effects of disruption of TH synthesis on swim bladder inflation during zebrafish early-life stages using 2-mercaptobenzothiazole (MBT), a thyroid peroxidase (TPO) inhibitor. Zebrafish were exposed to different MBT concentrations until 120/168 h post fertilization (hpf) and 32 days post fertilization (dpf), in two sets of experiments, to investigate the effects of TPO inhibition on posterior and anterior swim bladder inflation respectively, as well as whole body thyroid hormone concentrations (triiodothyronine (T3) and its prohormone, thyroxine (T4)). At 120 hpf, MBT did not directly impair posterior chamber inflation or size, while anterior chamber inflation and size was impaired at 32 dpf. As previously shown in amphibians and mammals, we confirmed that MBT inhibits TPO in fish. Whole-body T4 decreased after MBT exposure at both time points, while T3 levels were unaltered. There was a significant relationship between T4 levels and the anterior chamber surface at 32 dpf. The absence of effects on posterior chamber inflation can possibly be explained by maternal transfer of T4 into the eggs. These maternally derived THs are depleted at 32 dpf and cannot offset TPO inhibition, resulting in impaired anterior chamber inflation. Therefore, we hypothesize that TPO inhibition only inhibits swim bladder inflation during late development, after depletion of maternally derived T4. In a previous study, we showed that iodothyronine deiodinase (ID) knockdown impaired posterior chamber inflation during early development. Our findings, in parallel with similar effects observed in fathead minnow (see part I, this issue) suggest that thyroid disruption impacts swim bladder inflation, and imply an important distinction among specific subtypes of TH disrupting chemicals. However, the existence of another - yet unknown - mode of action of MBT impacting swim bladder inflation cannot be excluded. These results can be helpful for delineating adverse outcome pathways (AOPs) linking TPO inhibition, ID inhibition and other TH related molecular initiating events, to impaired swim bladder inflation in fish during early life stages. Such AOPs can support the use of in vitro enzyme inhibition assays for predicting reduced survival due to impaired posterior and anterior chamber inflation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Stinckens, Evelyn; Vergauwen, Lucia; Knapen, Dries] Univ Antwerp, Dept Vet Sci, Zebrafishlab Vet Physiol & Biochem, Univ Pl 1, B-2610 Antwerp, Belgium. [Schroeder, Anthony L.; Blackwell, Brett R.; Ankley, Gerald T.; Villeneuve, Daniel L.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Mid Continent Ecol Div, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Maho, Walid; Covaci, Adrian] Univ Antwerp, Dept Pharmaceut Sci, Toxicol Ctr, Univ Pl 1, B-2610 Antwerp, Belgium. [Witters, Hilda] Flemish Inst Technol Res VITO, Appl Bio & Mol Syst ABS, Boeretang 200, B-2400 Mol, Belgium. [Blust, Ronny] Univ Antwerp, Dept Biol, Syst Physiol & Ecotoxicol Res SPHERE, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. [Schroeder, Anthony L.] Univ Minnesota Twin Cities, Water Resources Ctr, 1985 Lower Buford Circle, St Paul, MN 55108 USA. RP Knapen, D (reprint author), ZEBRAFISHLAB Vet Physiol & Biochem, Dept Vet Sci, Campus Drie Eiken,Univ Pl 1,UC 173, B-2610 Antwerp, Belgium. EM dries.knapen@uantwerpen.be RI Covaci, Adrian/A-9058-2008; OI Covaci, Adrian/0000-0003-0527-1136; Vergauwen, Lucia/0000-0002-6534-7473; Knapen, Dries/0000-0003-0472-8114 FU Cefic Long-range Research Initiative [LRI-ECO20-UA]; ECETOC FX This work was funded by the Cefic Long-range Research Initiative (http://www.cefic-lri.org/) project LRI-ECO20-UA (Development of an alternative testing strategy for the fish early life-stage test for predicting chronic toxicity) with support of ECETOC. The views expressed in this paper are those of the authors and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 61 TC 1 Z9 1 U1 6 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-445X EI 1879-1514 J9 AQUAT TOXICOL JI Aquat. Toxicol. PD APR PY 2016 VL 173 BP 204 EP 217 DI 10.1016/j.aquatox.2015.12.023 PG 14 WC Marine & Freshwater Biology; Toxicology SC Marine & Freshwater Biology; Toxicology GA DH3MA UT WOS:000372689900020 PM 26818709 ER PT J AU Jaoui, M Lewandowski, M Docherty, KS Corse, EW Lonneman, WA Offenberg, JH Kleindienst, TE AF Jaoui, M. Lewandowski, M. Docherty, K. S. Corse, E. W. Lonneman, W. A. Offenberg, J. H. Kleindienst, T. E. TI Photooxidation of farnesene mixtures in the presence of NOx: Analysis of reaction products and their implication to ambient PM2.5 SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Sesquiterpenes; alpha-Farnesene; beta-Farnesene; PM2.5; SOA; Conjugated triene-ol ID SECONDARY ORGANIC AEROSOL; SUPERFICIAL SCALD DEVELOPMENT; BRANCH ENCLOSURE MEASUREMENTS; GAS-PHASE REACTION; UNITED-STATES; SESQUITERPENE EMISSIONS; OXYGENATED COMPOUNDS; VOLATILE EMISSIONS; CARBONYL PRODUCTS; RATE COEFFICIENT AB Chemical analysis of SOA produced from the irradiation of a mixture of alpha/beta-farnesene/NOx was conducted in a 14.5 m(3) smog chamber. SOA collected on glass-fiber filters was solvent extracted, derivatized using BSTFA, and analyzed by GC MS. Gas-phase products were analyzed using a combination of GC-FID and GC MS. This analysis showed the occurrence of more than 30 SOA oxygenated species and more than 20 in the gas phase. The major SOA components measured include conjugated alpha-farnesene trienols, C3-C7 linear dicarboxylic acids, carbonyl compounds, and hydroxy/carbonyl/carboxylic compounds. In the gas phase, the main species identified were formaldehyde, glyoxal, methylglyoxal, acetone, 2,3-dimethyl-oxirane, 2(3H)-furanone, 2-butenedioic acid, 4-oxopentanal, 4-methylenehex-5-enal, and 6-methylhept-5-en-2-one. Proposed reaction schemes are provided for selected compounds. H-atom abstraction and OH addition in alpha-farnesene oxidation seem to play an important role via the formation of unsaturated radicals containing different numbers of delocalized electrons. Allylic hydrogen abstraction and hydroperoxyalkyl radical channels might play a key role in the oxidation of alpha-farnesene. The contribution of farnesene SOA products to ambient PM2.5 was investigated by analyzing PM2.5 samples collected during SOAS 2013 field study at a site in Research Triangle Park (RTP), NC. The importance of these findings was supported by the occurrence of several organic species in both field and laboratory samples, suggesting the impact of farnesene on the ambient aerosol burden, mainly in areas where farnesene emissions are high. Although, pentanedioic acid and methylsuccinic acid appear to be candidate markers for farnesene SOA, additional chamber and mechanistic studies are required to estimate the contributions of farnesene to ambient SOA. (C) 2015 Published by Elsevier Ltd. C1 [Jaoui, M.; Docherty, K. S.; Corse, E. W.] Alion Sci & Technol, POB 12373, Durham, NC 27709 USA. [Lewandowski, M.; Offenberg, J. H.; Kleindienst, T. E.] US EPA, Res Triangle Pk, NC 27711 USA. [Lonneman, W. A.] US ES EPA, Senior Environm Employee Program, Res Triangle Pk, NC 27711 USA. [Jaoui, M.] Sci Syst & Applicat Inc, New York, NY 10010 USA. RP Jaoui, M (reprint author), Alion Sci & Technol, POB 12373, Durham, NC 27709 USA.; Jaoui, M (reprint author), Sci Syst & Applicat Inc, New York, NY 10010 USA. EM jaoui.mohammed@epa.gov RI Offenberg, John/C-3787-2009 OI Offenberg, John/0000-0002-0213-4024 FU U.S. Environmental Protection Agency through Office of Research and Development [EP-D-10-070, EP-C-15-008] FX The U.S. Environmental Protection Agency through its Office of Research and Development funded and collaborated in the research described here under Contract EP-D-10-070 to Alion Science and Technology and EP-C-15-008 to Jacobs Technology, Inc. The manuscript has been subjected to external peer review and has been cleared for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 42 TC 0 Z9 0 U1 15 U2 26 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD APR PY 2016 VL 130 SI SI BP 190 EP 201 DI 10.1016/j.atmosenv.2015.10.091 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DG9CU UT WOS:000372381200021 ER PT J AU Wu, SS Heberling, MT AF Wu, Shanshan Heberling, Matthew T. TI Estimating Green Net National Product for Puerto Rico: An Economic Measure of Sustainability SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Sustainability; Puerto Rico; Green Net National Product; Green accounting; Technological progress; Business cycle ID SAN-LUIS BASIN; REGIONAL SUSTAINABILITY; GENUINE SAVINGS; METRICS; INCOME; INDICATORS; RESOURCES; POLLUTION; PORTUGAL; DAMAGES AB This paper presents the data sources and methodology used to estimate Green Net National Product (GNNP), an economic metric of sustainability, for Puerto Rico. Using the change in GNNP as a one-sided test of weak sustainability (i.e., positive growth in GNNP is not enough to show the economy is sustainable), we measure the movement away from sustainability by examining the change in GNNP from 1993 to 2009. In order to calculate GNNP, we require both economic and natural capital data, but limited data for Puerto Rico require a number of simplifying assumptions. Based on the environmental challenges faced by Puerto Rico, we include damages from air emissions and solid waste, the storm protection value of mangroves and the value of extracting crushed stone as components in the depreciation of natural capital. Our estimate of GNNP also includes the value of time, which captures the effects of technological progress. The results show that GNNP had an increasing trend over the 17 years studied with two periods of negative growth (2004-2006 and 2007-2008). Our additional analysis suggests that the negative growth in 2004-2006 was possibly due to a temporary economic downturn. However, the negative growth in 2007-2008 was likely from the decline in the value of time, suggesting the island of Puerto Rico was moving away from sustainability during this time. C1 [Wu, Shanshan] Zhongnan Univ Econ & Law, Sch Econ, East Lake High Tech Dev Zone, 182 Nanhu Ave, Wuhan 430073, Peoples R China. [Heberling, Matthew T.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Sustainable Technol Div, Cincinnati, OH 45268 USA. RP Wu, SS (reprint author), Zhongnan Univ Econ & Law, Sch Econ, East Lake High Tech Dev Zone, 182 Nanhu Ave, Wuhan 430073, Peoples R China. EM swu@znufe.edu.cn FU National Research Council Research FX The views expressed herein are strictly the opinion of the authors and in no manner represent or reflect current or planned policy by the USEPA. This research would not have been possible without the assistance from the Puerto Rico Governor's Office, Puerto Rico Planning Board, Puerto Rico Environmental Quality Board, Puerto Rico Department of Natural and Environmental Resources, USDA-Forest Service, and USEPA. We thank all the staff who helped us with data and the metric calculation. We also thank the Puerto Rico Sustainability Metrics research team and collaborators for feedback on previous drafts. Shanshan Wu would like to acknowledge that part of this research was performed while holding National Research Council Research Associateship Award at USEPA, Office of Research and Development, National Risk Management Research Laboratory. The information and data presented in this product were obtained from sources that are believed to be reliable. However, in many cases the quality of the information or data was not documented by those sources; therefore, no claim is made regarding their quality. NR 67 TC 0 Z9 0 U1 5 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD APR PY 2016 VL 57 IS 4 BP 822 EP 835 DI 10.1007/s00267-015-0649-z PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG9VO UT WOS:000372432100007 PM 26721472 ER PT J AU Douglas, GC Thirkill, TL Kumar, P Loi, M Hilborn, ED AF Douglas, Gordon C. Thirkill, Twanda L. Kumar, Priyadarsini Loi, Minerva Hilborn, Elizabeth D. TI Effect of microcystin-LR on human placental villous trophoblast differentiation in vitro SO ENVIRONMENTAL TOXICOLOGY LA English DT Article DE microcystin; human; in vitro; placenta; human chorionic gonadotropin ID HUMAN CHORIONIC-GONADOTROPIN; OXIDATIVE STRESS; CELLS; APOPTOSIS; EXPRESSION; TOXICITY; PROTEIN; TRANSFERASES; HEPATOCYTES; ASSOCIATION AB Microcystin-LR is a cyanobacterial toxin found in surface and recreational waters that inhibits protein phosphatases and may disrupt the cytoskeleton. Microcystins induce apoptosis in hepatocytes at 2.0 mu M. Nothing is known about the effects of microcystins on human placental trophoblast differentiation and function. The differentiation of villous trophoblasts to form syncytiotrophoblast occurs throughout pregnancy and is essential for normal placental and fetal development. To investigate the effects of microcystin, villous cytotrophoblasts were isolated from term placentas using an established method and exposed to microcystin-LR. Microcystin-LR below the cytotoxic dose of 25 mu M did not cause cell rounding or detachment, had no effect on apoptosis, and no effect on the morphological differentiation of mononucleated cytotrophoblasts to multinucleated syncytiotrophoblast. However, secretion of human chorionic gonadotropin (hCG) increased in a microcystin-LR dose-dependent manner. When incubated with l-buthionine sulphoximine (BSO) to deplete glutathione levels, trophoblast morphological differentiation proceeded normally in the presence of microcystin-LR. Microcystin-LR did not disrupt the trophoblast microtubule cytoskeleton, which is known to play a role in trophoblast differentiation. Immunofluorescence studies showed that trophoblasts express organic anion transport protein 1B3 (OATP1B3), a known microcystin transport protein. In comparison to hepatocytes, trophoblasts appear to be more resistant to the toxic effects of microcystin-LR. The physiological implications of increased hCG secretion in response to microcystin-LR exposure remain to be determined. (c) 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 427-439, 2016. C1 [Douglas, Gordon C.; Thirkill, Twanda L.; Kumar, Priyadarsini; Loi, Minerva] Univ Calif Davis, Dept Cell Biol & Human Anat, Sch Med, Davis, CA 95616 USA. [Hilborn, Elizabeth D.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. RP Hilborn, ED (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. EM hilborn.e@epa.gov FU [EPD-10-052]; [EP-12-D-000023] FX The authors thank Edward Hudgens, USEPA, Office of Research and Development for technical support; Michael Narotsky, USEPA, Office of Research and Development for his technical guidance and thoughtful review. Contract #: EPD-10-052, EP-12-D-000023. The views expressed in this report are those of the individual authors and do not necessarily reflect the views and policies of the U.S. Environmental Protection Agency. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 42 TC 0 Z9 0 U1 7 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1520-4081 EI 1522-7278 J9 ENVIRON TOXICOL JI Environ. Toxicol. PD APR PY 2016 VL 31 IS 4 BP 427 EP 439 DI 10.1002/tox.22056 PG 13 WC Environmental Sciences; Toxicology; Water Resources SC Environmental Sciences & Ecology; Toxicology; Water Resources GA DG8FL UT WOS:000372318300005 PM 25346179 ER PT J AU Batt, AL Kincaid, TM Kostich, MS Lazorchak, JM Olsen, AR AF Batt, Angela L. Kincaid, Thomas M. Kostich, Mitchell S. Lazorchak, James M. Olsen, Anthony R. TI EVALUATING THE EXTENT OF PHARMACEUTICALS IN SURFACE WATERS OF THE UNITED STATES USING A NATIONAL-SCALE RIVERS AND STREAMS ASSESSMENT SURVEY SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Pharmaceuticals; Water quality; Environmental chemistry; Emerging pollutants ID WASTE-WATER; STEROID-HORMONES; AQUATIC ENVIRONMENT; FISH; CONTAMINATION; RESOURCES; SEROTONIN; US; BIOACCUMULATION; ECOTOXICOLOGY AB To assess the potential exposure of aquatic ecosystems to active pharmaceutical ingredients, the authors conducted a national-scale, probability-based statistical survey of the occurrence of these compounds in surface waters of the United States. The survey included 182 sampling sites and targeted rivers with close proximity to urban areas. The 46 analytes reported represent many classes of active pharmaceutical ingredients (APIs), including antibiotics, diuretics, antihypertensives, anticonvulsants, and antidepressants. Of the 46 analytes, 37 were detected in at least 1 sampling location. Sulfamethoxazole (an antibiotic) was the most frequently detected compound, being measured in 141 of the 182 surface waters surveyed at concentrations ranging up to 570 ng/L. Ten of the compounds were detected in 20% or more of the sampling sites. Weighted means of the analytical measurements are used with the statistical survey design and analysis to provide national estimates of the extent of contamination for these APIs in the nation's urban rivers. Published 2015 Wiley Periodicals, Inc. on behalf of SETAC. This article is a US Government work and as such, is in the public domain in the United States of America. C1 [Batt, Angela L.; Kostich, Mitchell S.; Lazorchak, James M.] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. [Kincaid, Thomas M.; Olsen, Anthony R.] US EPA, Off Res & Dev, Corvallis, OR USA. RP Batt, AL (reprint author), US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. EM batt.angela@epa.gov OI Lazorchak, James/0000-0002-7354-7571 NR 43 TC 3 Z9 3 U1 13 U2 37 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD APR PY 2016 VL 35 IS 4 BP 874 EP 881 DI 10.1002/etc.3161 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DH0RB UT WOS:000372490300013 PM 26562077 ER PT J AU Laurin, L Amor, B Bachmann, TM Bare, J Koffler, C Genest, S Preiss, P Pierce, J Satterfield, B Vigon, B AF Laurin, Lise Amor, Ben Bachmann, Till M. Bare, Jane Koffler, Christoph Genest, Serge Preiss, Philipp Pierce, Jason Satterfield, Barclay Vigon, Bruce TI Life cycle assessment capacity roadmap (section 1): decision-making support using LCA SO INTERNATIONAL JOURNAL OF LIFE CYCLE ASSESSMENT LA English DT Letter DE Decision-making support; Impact categories; Life cycle analysis; Life cycle assessment; Multi-criteria decision analysis; Normalization; Uncertainty; Visualization ID IMPACT ASSESSMENT; NORMALIZATION AB When life cycle assessment (LCA) results do not show a clear and certain environmental preference of one choice over one or several alternatives, current methods are limited in their ability to inform decision-makers. To address this and related cross-cutting issues, a group of LCA practitioners has been working on a roadmap for capacity development in LCA. The roadmap is identifying common needs for development in LCA, which can then be addressed by the broader LCA community. The roadmap document on decision-making support, having undergone a public comment period, outlines the current state as well as needs and milestones to ensure progress continues apace. The roadmap document, available for download, covers five main areas of development: (1) performance measures of confidence, which identify the acceptable uncertainty for study results, while minimizing expenditures; (2) selection of impact categories, an area with multiple existing methods. The roadmap suggests codifying these methods and identifying their suitability to various applications; (3) normalization; while several methods of normalization are in use, the method with the greatest acceptance in the LCA community (i.e., relying on total or per capita regional emissions/extractions) has a number of methodological drawbacks; (4) weighting, which is a form of multi-criteria decision analysis (MCDA). The broader MCDA field can enrich LCA by providing studied methods of assessing trade-offs; and (5) visualization of results. Many other LCA capacity needs would benefit from documentation. These include but are not limited to the following: addressing ill-characterized uncertainty, life cycle inventory data needs, data format needs, and tool capabilities. Other roadmapping groups are forming and are looking for practitioners to support the effort. C1 [Laurin, Lise] EarthShift Global, 37 Route 236,Suite 112, Kittery, ME 03904 USA. [Amor, Ben] Univ Sherbrooke, Dept Civil Engn, 2500 Boul Univ, Sherbrooke, PQ J1K 2R1, Canada. [Bachmann, Till M.; Preiss, Philipp] European Inst Energy Res EIFER, Emmy Noether Str 11, D-76131 Karlsruhe, Germany. [Bare, Jane] US EPA, LCA Res Ctr, Off Res & Dev, 26 W MLK Dr, Cincinnati, OH 45268 USA. [Koffler, Christoph] Thinkstep Inc, 170 Milk St, Boston, MA 02109 USA. [Genest, Serge] FPInnovat Environm & Sustainabil, 570 Boul St Jean, Pointe Claire, PQ H9R 3J9, Canada. [Pierce, Jason] Eastman Chem Co, 200 S Wilcox Dr, Kingsport, TN 37660 USA. [Satterfield, Barclay] Eastman Innovat Ctr, Eastman Chem Co, 840 Main Campus Dr,Suite 2100, Raleigh, NC 27606 USA. [Vigon, Bruce] Soc Environm Toxicol & Chem, 229 Baylen St, Pensacola, FL 32502 USA. RP Laurin, L (reprint author), EarthShift Global, 37 Route 236,Suite 112, Kittery, ME 03904 USA.; Vigon, B (reprint author), Soc Environm Toxicol & Chem, 229 Baylen St, Pensacola, FL 32502 USA. EM lise@earthshiftglobal.com; bruce.vigon@setac.org OI Amor, Mourad Ben/0000-0002-0389-5685 NR 9 TC 0 Z9 0 U1 5 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0948-3349 EI 1614-7502 J9 INT J LIFE CYCLE ASS JI Int. J. Life Cycle Assess. PD APR PY 2016 VL 21 IS 4 BP 443 EP 447 DI 10.1007/s11367-016-1031-y PG 5 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DG8HO UT WOS:000372324200001 ER PT J AU Seth, A Klise, KA Siirola, JD Haxton, T Laird, CD AF Seth, Arpan Klise, Katherine A. Siirola, John D. Haxton, Terranna Laird, Carl D. TI Testing Contamination Source Identification Methods for Water Distribution Networks SO JOURNAL OF WATER RESOURCES PLANNING AND MANAGEMENT LA English DT Article DE Drinking water distribution system; Source identification; Testing ID DISTRIBUTION-SYSTEMS; OPTIMIZATION; ALGORITHM; SECURITY; DESIGN; MODEL AB In the event of contamination in a water distribution network (WDN), source identification (SI) methods that analyze sensor data can be used to identify the source location(s). Knowledge of the source location and characteristics are important to inform contamination control and cleanup operations. Various SI strategies that have been developed by researchers differ in their underlying assumptions and solution techniques. The following manuscript presents a systematic procedure for testing and evaluating SI methods. The performance of these SI methods is affected by various factors including the size of WDN model, measurement error, modeling error, time and number of contaminant injections, and time and number of measurements. This paper includes test cases that vary these factors and evaluates three SI methods on the basis of accuracy and specificity. The tests are used to review and compare these different SI methods, highlighting their strengths in handling various identification scenarios. These SI methods and a testing framework that includes the test cases and analysis tools presented in this paper have been integrated into EPA's Water Security Toolkit (WST), a suite of software tools to help researchers and others in the water industry evaluate and plan various response strategies in case of a contamination incident. Finally, a set of recommendations are made for users to consider when working with different categories of SI methods. C1 [Seth, Arpan; Laird, Carl D.] Purdue Univ, Sch Chem Engn, 480 Stadium Mall, W Lafayette, IN 47907 USA. [Klise, Katherine A.] Sandia Natl Labs, Geosci Res & Applicat Grp, POB 5800,MS 0751, Albuquerque, NM 87185 USA. [Siirola, John D.] Sandia Natl Labs, Ctr Res Comp, POB 5800 MS,1326, Albuquerque, NM 87185 USA. [Haxton, Terranna] US EPA, Natl Homeland Secur Res Ctr, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. RP Laird, CD (reprint author), Purdue Univ, Sch Chem Engn, 480 Stadium Mall, W Lafayette, IN 47907 USA. EM haxton.terra@epa.gov; lairdc@purdue.edu FU Sandia National Laboratories; U.S. Environmental Protection Agency; Lockheed Martin Corporation for the U.S. Department of Energy's National Nuclear Security Administration [DE-AC04 94AL85000]; U.S. Environmental Protection Agency (EPA) through its Office of Research and Development [DW8992192801]; Department of Energy's Sandia National Laboratories; [SAND2014-20020 J] FX The authors gratefully acknowledge financial support provided by Sandia National Laboratories and U.S. Environmental Protection Agency. Sandia National Laboratories is a multiprogram laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04 94AL85000. SAND Number SAND2014-20020 J. The U.S. Environmental Protection Agency (EPA) through its Office of Research and Development funded and collaborated in the research described here under an Interagency Agreement (IA # DW8992192801) with the Department of Energy's Sandia National Laboratories. It has been subject to an administrative review but does not necessarily reflect the views of the Agency. No official endorsement should be inferred. EPA does not endorse the purchase or sale of any commercial products or services. NR 32 TC 1 Z9 1 U1 3 U2 11 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9496 EI 1943-5452 J9 J WATER RES PLAN MAN JI J. Water Resour. Plan. Manage.-ASCE PD APR PY 2016 VL 142 IS 4 AR 04016001 DI 10.1061/(ASCE)WR.1943-5452.0000619 PG 11 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA DH5DD UT WOS:000372804500003 ER PT J AU Zabaloy, MC Garland, JL Allegrini, M Gomez, ED AF Celina Zabaloy, Maria Garland, Jay L. Allegrini, Marco del Valle Gomez, Elena TI Soil Microbial Community-Level Physiological Profiling as Related to Carbon and Nitrogen Availability Under Different Land Uses SO PEDOSPHERE LA English DT Article DE community-level physiological profiling; N limitation; oxygen biosensor system; soil organic C; soil respiration ID LIMITING BACTERIAL-GROWTH; NUTRIENT LIMITATIONS; CATABOLIC DIVERSITY; RESPIRATION; TILLAGE; BIOMASS; FOREST; FERTILIZATION; DECOMPOSITION; SUBSTRATE AB The goal of this work was to assess soil microbial respiration, determined by the assay of community-level physiological profiling in an oxygen-sensitive microplate (O-2-CLPP), in response to endogenous C and several individual C substrates in the soils with different organic C contents (as a function of soil type and management practice). We also used the O-2-CLPP to determine the respiratory response of these soils to endogenous C and amended C substrates with N addition. A respiratory quotient (RQ) was calculated based on the ratio of the response to endogenous soil C vs. each C-only substrate, and was related to total organic carbon (TOC). For assessing N availability for microbial activity, the effect of N supplementation on soil respiration, expressed as N-ratio, was calculated based on the response of several substrates to N addition relative to the response without N. Soils clustered in 4 groups after a principal component analysis (PCA), based on TOC and their respiratory responses to substrates and endogenous C. These groups reflected differences among soils in their geographic origin, land use and C content. Calculated RQ values were significantly lower in natural forest soils than in managed soils for most C-only substrates. TOC was negatively correlated with RQ (r = 0.65), indicating that the soils with higher organic matter content increased respiratory efficiency. The N addition in the assay in the absence of C amendment (i.e., only endogenous soil C present) had no effect on microbial respiration in any soil, indicating that these soils were not intrinsically N-limited, but substrate-dependent variation in Nratio within soil groups was observed. C1 [Celina Zabaloy, Maria] Consejo Nacl Invest Cient & Tecn, Ctr Recursos Nat Renovables Zona Semiarida, RA-8000 Bahia Blanca, Buenos Aires, Argentina. [Garland, Jay L.] US EPA, Microbiol & Chem Exposure Assessment Res Div, Cincinnati, OH 45268 USA. [Allegrini, Marco; del Valle Gomez, Elena] Univ Nacl Rosario, Fac Ciencias Agr, RA-2125 Zavalla, Argentina. RP Zabaloy, MC (reprint author), Consejo Nacl Invest Cient & Tecn, Ctr Recursos Nat Renovables Zona Semiarida, RA-8000 Bahia Blanca, Buenos Aires, Argentina. EM mzabaloy@uns.edu.ar NR 51 TC 1 Z9 1 U1 7 U2 31 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1002-0160 EI 2210-5107 J9 PEDOSPHERE JI Pedosphere PD APR PY 2016 VL 26 IS 2 BP 216 EP 225 DI 10.1016/S1002-0160(15)60036-4 PG 10 WC Soil Science SC Agriculture GA DH4NP UT WOS:000372763000007 ER PT J AU Lytle, DA Liggett, J AF Lytle, Darren A. Liggett, Jennifer TI Impact of water quality on chlorine demand of corroding copper SO WATER RESEARCH LA English DT Article DE Copper; Chlorine; Oxidant demand; Orthophosphate; Drinking water ID DRINKING-WATER; DISTRIBUTION-SYSTEMS; PITTING CORROSION; HIGH-PH; ALKALINITY; OXIDES; DECAY; PIPE AB Copper is widely used in drinking water premise plumbing system materials. In buildings such as hospitals, large and complicated plumbing networks make it difficult to maintain good water quality. Sustaining safe disinfectant residuals throughout a building to protect against waterborne pathogens such as Legionella is particularly challenging since copper and other reactive distribution system materials can exert considerable demands. The objective of this work was to evaluate the impact of pH and orthophosphate on the consumption of free chlorine associated with corroding copper pipes over time. A copper test-loop pilot system was used to control test conditions and systematically meet the study objectives. Chlorine consumption trends attributed to abiotic reactions with copper over time were different for each pH condition tested, and the total amount of chlorine consumed over the test runs increased with increasing pH. Orthophosphate eliminated chlorine consumption trends with elapsed time (i.e., chlorine demand was consistent across entire test runs). Orthophosphate also greatly reduced the total amount of chlorine consumed over the test runs. Interestingly, the total amount of chlorine consumed and the consumption rate were not pH dependent when orthophosphate was present. The findings reflect the complex and competing reactions at the copper pipe wall including corrosion, oxidation of Cu(I) minerals and ions, and possible oxidation of Cu(II) minerals, and the change in chlorine species all as a function of pH. The work has practical applications for maintaining chlorine residuals in premise plumbing drinking water systems including large buildings such as hospitals. Published by Elsevier Ltd. C1 [Lytle, Darren A.] US EPA, Water Supply & Water Resources Div, Natl Risk Management Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. [Liggett, Jennifer] Univ Cincinnati, Coll Engn & Appl Sci, Dept 2600 Clifton Ave, Cincinnati, OH 45220 USA. RP Lytle, DA (reprint author), US EPA, Water Supply & Water Resources Div, Natl Risk Management Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. EM Lytle.Darren@epa.gov NR 41 TC 3 Z9 3 U1 5 U2 43 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD APR 1 PY 2016 VL 92 BP 11 EP 21 DI 10.1016/j.watres.2016.01.032 PG 11 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DF7RL UT WOS:000371555200002 PM 26826646 ER PT J AU Fortenberry, GZ Beckman, J Schwartz, A Prado, JB Graham, LS Higgins, S Lackovic, M Mulay, P Bojes, H Waltz, J Mitchell, Y Leinenkugel, K Oriel, MS Evans, E Calvert, GM AF Fortenberry, Gamola Z. Beckman, John Schwartz, Abby Prado, Joanne Bonnar Graham, Lucia S. Higgins, Sheila Lackovic, Michelle Mulay, Prakash Bojes, Heidi Waltz, Justin Mitchell, Yvette Leinenkugel, Kathy Oriel, Michel S. Evans, Elizabeth Calvert, Geoffrey M. TI Magnitude and characteristics of acute paraquat- and diquat-related illnesses in the US: 1998-2013 SO ENVIRONMENTAL RESEARCH LA English DT Article DE Paraquat; Diquat; Pesticides; Acute Poisonings; Surveillance ID CLINICAL-FEATURES; FORMULATION; MANAGEMENT; INGESTION AB Background: Paraquat and diquat are among the most commonly used herbicides in the world. Objectives: Determine the magnitude, characteristics, and root causes for acute paraquat- and diquatrelated illnesses in the US Methods: Illnesses associated with paraquat or diquat exposure occurring from 1998 through 2011 were identified from the Sentinel Event Notification System for Occupational Risks (SENSOR)-Pesticides Program, the California Department of Pesticide Regulation (CDPR) Pesticide Illness Surveillance Program (PISP), and the Incident Data System (IDS). Cases identified by the National Poison Data System (NPDS) were reviewed for the years 1998-2003 and 2006-2013. Results: A total of 300 paraquat- and 144 diquat-related acute illnesses were identified by SENSOR, PISP, and IDS. NPDS identified 693 paraquat- and 2128 diquat-related acute illnesses. In SENSOR/PISP/IDS, illnesses were commonly low severity (paraquat=41%; diquat=81%); however, SENSOR/PISP/IDS identified 24 deaths caused by paraquat and 5 deaths associated with diquat. Nineteen paraquat-related deaths were due to ingestion, seven of which were unintentional, often due to improper storage in beverage bottles. In SENSOR/PISP/IDS, paraquat and diquat-related acute illnesses were work-related in 68% (n=203) and 29% (n=42) of cases, respectively. When herbicide application site was known, the vast majority of acute paraquat-related illnesses (81%) arose from agricultural applications. Common root causes of illness were failure to use adequate personal protective equipment (PPE), application equipment failure, and spill/splash of herbicide. Conclusions: Although the magnitude of acute paraquat/diquat-related illnesses was relatively low, several fatalities were identified. Many illnesses could be prevented through stricter compliance with label requirements (e.g. ensuring proper herbicide storage and PPE use), and through enhanced training of certified applicators. Published by Elsevier Inc. C1 [Fortenberry, Gamola Z.; Calvert, Geoffrey M.] NIOSH, Div Surveillance Hazard Evaluat & Field Studies, Ctr Dis Control & Prevent, Cincinnati, OH 45226 USA. [Beckman, John] Inst Publ Hlth, Oakland, CA USA. [Beckman, John] Calif Dept Publ Hlth, Richmond, CA USA. [Schwartz, Abby] Michigan Dept Hlth & Human Serv, Lansing, MI USA. [Prado, Joanne Bonnar] Washington State Dept Hlth, Olympia, WA USA. [Graham, Lucia S.; Oriel, Michel S.] Calif Dept Pesticide Regulat, Sacramento, CA USA. [Higgins, Sheila] North Carolina Dept Hlth & Human Serv, Raleigh, NC USA. [Lackovic, Michelle] Louisiana Dept Hlth & Hosp, New Orleans, LA USA. [Mulay, Prakash] Florida Dept Hlth, Tallahassee, FL USA. [Bojes, Heidi] Texas Dept State Hlth Serv, Austin, TX USA. [Waltz, Justin] Oregon Hlth Author, Portland, OR USA. [Mitchell, Yvette] New York State Dept Hlth, Albany, NY USA. [Leinenkugel, Kathy] Iowa Dept Publ Hlth, Des Moines, IA 50319 USA. [Evans, Elizabeth] US EPA, Off Pesticide Programs, Washington, DC 20460 USA. RP Calvert, GM (reprint author), NIOSH, Div Surveillance Hazard Evaluat & Field Studies, Ctr Dis Control & Prevent, Cincinnati, OH 45226 USA. EM jac6@cdc.gov FU National Institute for Occupational Safety and Health, United States; United States Environmental Protection Agency (EPA) [DW-75-95840001-2] FX Funding was provided by the National Institute for Occupational Safety and Health, United States, the United States Environmental Protection Agency (EPA Interagency Agreement identification number DW-75-95840001-2), and the participating state agencies that provided data. NR 24 TC 1 Z9 2 U1 2 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0013-9351 EI 1096-0953 J9 ENVIRON RES JI Environ. Res. PD APR PY 2016 VL 146 BP 191 EP 199 DI 10.1016/j.envres.2016.01.003 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DF2TE UT WOS:000371196000023 PM 26775000 ER PT J AU Platten, WE Sylvest, N Warren, C Arambewela, M Harmon, S Bradham, K Rogers, K Thomas, T Luxton, TP AF Platten, William E., III Sylvest, Nicholas Warren, Casey Arambewela, Mahendranath Harmon, Steve Bradham, Karen Rogers, Kim Thomas, Treye Luxton, Todd Peter TI Estimating dermal transfer of copper particles from the surfaces of pressure-treated lumber and implications for exposure SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Pressure-treated wood; Nanoparticle; Copper exposure; Micronized copper azole; Ionic copper; Copper speciation ID HEALTH-RISK ASSESSMENT; OXIDE NANOPARTICLES; WOOD PRESERVATIVES; DRINKING-WATER; TOXICITY; SEAWATER; KINETICS AB Lumber pressure-treated with micronized copper was examined for the release of copper and copper micro/nanoparticles using a surface wipe method to simulate dermal transfer. In 2003, the wood industry began replacing CCA treated lumber products for residential use with copper based formulations. Micronized copper (nano to micron sized particles) has become the preferred treatment formulation. There is a lack of information on the release of copper, the fate of the particles during dermal contact, and the copper exposure level to children from hand-to-mouth transfer. For the current study, three treated lumber products, two micronized copper and one ionic copper, were purchased from commercial retailers. The boards were left to weather outdoors for approximately 1 year. Over the year time period, hand wipe samples were collected periodically to determine copper transfer from the wood surfaces. The two micronized formulations and the ionic formulation released similar levels of total copper. The amount of copper released was high initially, but decreased to a constant level (similar to 1.5 mg m(-2)) after the first month of outdoor exposure. Copper particles were identified on the sampling cloths during the first two months of the experiment, after which the levels of copper were insufficient to collect interpretable data. After 1 month, the particles exhibited minimal changes in shape and size. At the end of 2-months, significant deterioration of the particles was evident. Based on the wipe sample data, a playground visit may result in a potential exposure to 2.58 mg of copper, which is near or exceeds the daily tolerable upper intake limits for children under the age of 8, if completely ingested through hand-to-mouth transfer. While nanoparticles were found, there is not enough information to estimate the exposure from the released particles due to a lack of published literature on copper carbonate. Published by Elsevier B.V. C1 [Platten, William E., III; Sylvest, Nicholas; Warren, Casey; Arambewela, Mahendranath] Pegasus Tech Serv Inc, 46 E Hollister St, Cincinnati, OH 45219 USA. [Harmon, Steve; Luxton, Todd Peter] US EPA, Natl Risk Management Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. [Bradham, Karen; Rogers, Kim] US EPA, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Thomas, Treye] US Consumer Prod Safety Commiss, Off Hazard Identificat & Reduct, 4330 East West Highway Suite 600, Bethesda, MD 20814 USA. RP Luxton, TP (reprint author), US EPA, Natl Risk Management Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. EM Luxton.Todd@epa.gov RI BM, MRCAT/G-7576-2011 FU Pegasus Technical Services, Inc. [EP-C-11-006]; DOE Office of Science by Argonne National Laboratory [DE-AC02-06CH11357] FX This work was part of an interagency agreement between the U.S. EPA and the U.S. CPSC. Research support was provided by Pegasus Technical Services, Inc. to EPA through Contract EP-C-11-006 Work Assignment 73. This work has been subjected to EPA administrative review and approved for publication. The findings and conclusions in this paper are those of the authors and do not necessarily represent the views of the CPSC or EPA. Mention of trade names or commercial products does not constitute endorsement or recommendation for use, nor does it imply that alternative products are unavailable or unable to be substituted after appropriate evaluation. The Advanced Photon Source (APS, Argonne, Illinois, USA) is acknowledged for the allocation of beam time. MRCAT operations are supported by the Department of Energy (DOE) and the MRCAT member institutions. This research used resources of the Advanced Photon Source, a U.S. DOE Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. NR 37 TC 3 Z9 3 U1 4 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD APR 1 PY 2016 VL 548 BP 441 EP 449 DI 10.1016/j.scitotenv.2015.12.108 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DD9LC UT WOS:000370246000046 PM 26826852 ER PT J AU Little, MP Hendry, JH Puskin, JS AF Little, Mark P. Hendry, Jolyon H. Puskin, Jerome S. TI Lack of Correlation between Stem-Cell Proliferation and Radiation-or Smoking-Associated Cancer Risk SO PLOS ONE LA English DT Article ID GENOMIC INSTABILITY; COLON-CANCER; RELATIVE RISK; CARCINOGENESIS; MODEL; MORTALITY; EXPOSURE; LUNG; MUTATIONS; ARMITAGE AB Background A recent paper by Tomasetti and Vogelstein (Science 2015 347 78-81) suggested that the variation in natural cancer risk was largely explained by the total number of stem-cell divisions, and that most cancers arose by chance. They proposed an extra-risk score as way of distinguishing the effects of the stochastic, replicative component of cancer risk from other causative factors, specifically those due to the external environment and inherited mutations. Objectives We tested the hypothesis raised by Tomasetti and Vogelstein by assessing the degree of correlation of stem cell divisions and their extra-risk score with radiation-and tobacco-associated cancer risk. Methods We fitted a variety of linear and log-linear models to data on stem cell divisions per year and cumulative stem cell divisions over lifetime and natural cancer risk, some taken from the paper of Tomasetti and Vogelstein, augmented using current US lifetime cancer risk data, and also radiation-and tobacco-associated cancer risk. Results The data assembled by Tomasetti and Vogelstein, as augmented here, are inconsistent with the power-of-age relationship commonly observed for cancer incidence and the predictions of a multistage carcinogenesis model, if one makes the strong assumption of homogeneity of numbers of driver mutations across cancer sites. Analysis of the extra-risk score and various other measures (number of stem cell divisions per year, cumulative number of stem cell divisions over life) considered by Tomasetti and Vogelstein suggests that these are poorly predictive of currently available estimates of radiation- or smoking-associated cancer risk-for only one out of 37 measures or logarithmic transformations thereof is there a statistically significant correlation (p<0.05) with radiation-or smoking-associated risk. Conclusions The data used by Tomasetti and Vogelstein are in conflict with predictions of a multistage model of carcinogenesis, under the assumption of homogeneity of numbers of driver mutations across most cancer sites. Their hypothesis that if the extra-risk score for a tissue type is high then one would expect that environmental factors would play a relatively more important role in that cancer's risk is in conflict with the lack of correlation between the extra-risk score and other stem-cell proliferation indices and radiation-or smoking-related cancer risk. C1 [Little, Mark P.] NCI, Radiat Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,DHHS, Rockville, MD USA. [Hendry, Jolyon H.] Christie Hosp, Christie Med Phys & Engn, Manchester, Lancs, England. [Hendry, Jolyon H.] Univ Manchester, Manchester, Lancs, England. [Puskin, Jerome S.] US EPA, Radiat Protect Div, Washington, DC 20460 USA. RP Little, MP (reprint author), NCI, Radiat Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,DHHS, Rockville, MD USA. EM mark.little@nih.gov OI Matsuo, Keitaro/0000-0003-1761-6314 FU Intramural Research Program of the National Institutes of Health, the National Cancer Institute, Division of Cancer Epidemiology and Genetics FX The work of MPL was supported by the Intramural Research Program of the National Institutes of Health, the National Cancer Institute, Division of Cancer Epidemiology and Genetics. NR 38 TC 4 Z9 4 U1 3 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 31 PY 2016 VL 11 IS 3 AR e0150335 DI 10.1371/journal.pone.0150335 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH9NC UT WOS:000373121800006 PM 27031507 ER PT J AU Ingwersen, WW Ceja, M Weisbrod, AV Cabezas, H Demeke, B Eason, T Smith, RL Sengupta, D Zanoli, E Gausman, M Lee, SJ Ma, X Weber, B Alvarez, M Bare, JC Abraham, J Ruiz-Mercado, GJ Gonzalez, MA AF Ingwersen, Wesley W. Ceja, Manuel Weisbrod, Anne V. Cabezas, Heriberto Demeke, Bayou Eason, Tarsha Smith, Raymond L. Sengupta, Debalina Zanoli, Ed Gausman, Maria Lee, Seung-Jin Ma, Xin (Cissy) Weber, Bernard Alvarez, Mauricio Bare, Jane C. Abraham, John Ruiz-Mercado, Gerardo J. Gonzalez, Michael A. TI Evaluating Consumer Product Life Cycle Sustainability with Integrated Metrics: A Paper Towel Case Study SO INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH LA English DT Article AB Integrated sustainability metrics provide an enriched set of information to inform decision-making. However, such approaches are rarely used to assess product supply chains. In this work, four integrated metrics presented in terms of land, resources, value added, and stability-are applied in a life cycle context, along with industrial process systems assessment and life cycle assessment, to evaluate Bounty paper towels from two manufacturing lines. The results show that the paper towels from the more state-of-the-art manufacturing line and newer facility are marginally more sustainable by the majority of environmental measures. Drivers of impacts from land use, resource use, and externality costs in the product life cycle are largely in the supply chains for raw materials and energy. The integrated metrics point to greenhouse gases, criteria air pollutants, land used for pulpwood, and fossil fuel use as important emissions and resources to manage for improving the sustainability of paper towel production. The metrics employed help to reconcile contrasting results from the life cycle impact assessment, such as water and energy use impacts, and provide a reduced set of practical, yet comprehensive, information to inform product-related decision-making. C1 [Ingwersen, Wesley W.; Cabezas, Heriberto; Demeke, Bayou; Eason, Tarsha; Smith, Raymond L.; Ma, Xin (Cissy); Bare, Jane C.; Abraham, John; Ruiz-Mercado, Gerardo J.; Gonzalez, Michael A.] US EPA, Natl Risk Management Res Lab, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Ceja, Manuel; Weisbrod, Anne V.; Zanoli, Ed; Gausman, Maria; Weber, Bernard; Alvarez, Mauricio] Procter & Gamble Co, Global Product Stewardship & Product Supply, Cincinnati, OH 45202 USA. [Sengupta, Debalina] Texas A&M Univ, Texas A&M Engn Expt Stn TEES, Gas & Fuels Res Ctr, College Stn, TX 77843 USA. [Lee, Seung-Jin] Univ Michigan Flint, Dept Earth & Resource Sci, Flint, MI 48502 USA. [Lee, Seung-Jin] Univ Michigan Flint, Dept Comp Sci Engn & Phys, Flint, MI 48502 USA. RP Cabezas, H (reprint author), US EPA, Natl Risk Management Res Lab, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM cabezas.heriberto@epa.gov FU (CRADA) USEPA's National Risk Management Research Laboratory [683-12]; Procter Gamble Company; USEPA FX A Cooperative Research and Development Agreement (CRADA, No. 683-12) between the USEPA's National Risk Management Research Laboratory and The Procter & Gamble Company enabled the collaboration that produced this research. This research was part of the Chemical Safety and Sustainability National Research Program in the USEPA's Office of Research and Development. Post-doctoral research fellowships with the USEPA managed by the Oak Ridge Institute for Science and Education enabled the participation of D.S. and S.-J.L. in this research. NR 24 TC 2 Z9 2 U1 7 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0888-5885 J9 IND ENG CHEM RES JI Ind. Eng. Chem. Res. PD MAR 30 PY 2016 VL 55 IS 12 BP 3433 EP 3441 DI 10.1021/acs.iecr.5b03743 PG 9 WC Engineering, Chemical SC Engineering GA DI5DO UT WOS:000373519300020 ER PT J AU Harding, LW Mallonee, ME Perry, ES Miller, WD Adolf, JE Gallegos, CL Paerl, HW AF Harding, Lawrence W., Jr. Mallonee, Michael E. Perry, Elgin S. Miller, W. David Adolf, Jason E. Gallegos, Charles L. Paerl, Hans W. TI Variable climatic conditions dominate recent phytoplankton dynamics in Chesapeake Bay SO SCIENTIFIC REPORTS LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; PARTIALLY STRATIFIED ESTUARY; RIVER FLOW; SYNOPTIC CLIMATOLOGY; PRIMARY PRODUCTIVITY; FLORAL COMPOSITION; NORTH-CAROLINA; WATER-QUALITY; EUTROPHICATION; COASTAL AB Variable climatic conditions strongly influence phytoplankton dynamics in estuaries globally. Our study area is Chesapeake Bay, a highly productive ecosystem providing natural resources, transportation, and recreation for nearly 16 million people inhabiting a 165,000-km(2) watershed. Since World War II, nutrient over-enrichment has led to multiple ecosystem impairments caused by increased phytoplankton biomass as chlorophyll-a (chl-a). Doubled nitrogen (N) loadings from 1945-1980 led to increased chl-a, reduced water clarity, and low dissolved oxygen (DO), while decreased N loadings from 1981-2012 suggest modest improvement. The recent 30+ years are characterized by high inter-annual variability of chl-a, coinciding with irregular dry and wet periods, complicating the detection of long-term trends. Here, we synthesize time-series data for historical and recent N loadings (TN, NO2 + NO3), chl-a, floral composition, and net primary productivity (NPP) to distinguish secular changes caused by nutrient over-enrichment from spatio-temporal variability imposed by climatic conditions. Wet years showed higher chl-a, higher diatom abundance, and increased NPP, while dry years showed lower chl-a, lower diatom abundance, and decreased NPP. Our findings support a conceptual model wherein variable climatic conditions dominate recent phytoplankton dynamics against a backdrop of nutrient over-enrichment, emphasizing the need to separate these effects to gauge progress toward improving water quality in estuaries. C1 [Harding, Lawrence W., Jr.] Univ Calif Los Angeles, Dept Atmospher & Ocean Sci, Los Angeles, CA 90095 USA. [Mallonee, Michael E.] US EPA, Interstate Commiss Potomac River Basin, Chesapeake Bay Program Off, 410 Severn Ave, Annapolis, MD 21403 USA. [Miller, W. David] US Naval Res Lab, 4555 Overlook Ave SW, Washington, DC 20375 USA. [Adolf, Jason E.] Univ Hawaii, Marine Sci Program, 200 W Kawili St, Hilo, HI 96720 USA. [Gallegos, Charles L.] Smithsonian Environm Res Ctr, 647 Contees Wharf Rd, Edgewater, MD 21037 USA. [Paerl, Hans W.] Univ North Carolina Chapel Hill, Inst Marine Sci, 3431 Arendell St, Morehead City, NC 28557 USA. RP Harding, LW (reprint author), Univ Calif Los Angeles, Dept Atmospher & Ocean Sci, Los Angeles, CA 90095 USA. EM lharding@atmos.ucla.edu OI Gallegos, Charles/0000-0001-5112-0166; Miller, W. David/0000-0002-4940-5987 FU National Science Foundation (NSF) Land Margin Ecosystem Research, Biocomplexity, Biological Oceanography, Microbial Observatory, and Small Grants for Environmental Research (SGER) programs; Atlantic Coast Environmental Indicators Consortium (ACE-INC) - EPA; Atlantic Coast Environmental Indicators Consortium (ACE-INC) - National Aeronautics and Space Administration (NASA); NASA SeaWiFS Program; SIMBIOS Program; Chesapeake Bay Program Office of the National Oceanic and Atmospheric Administration (NOAA) FX The Authors acknowledge support from the Chesapeake Bay Program of the US Environmental Protection Agency (EPA) for providing water-quality and cell-counts data; grants from the National Science Foundation (NSF) Land Margin Ecosystem Research, Biocomplexity, Biological Oceanography, Microbial Observatory, and Small Grants for Environmental Research (SGER) programs; the Atlantic Coast Environmental Indicators Consortium (ACE-INC) supported by EPA and the National Aeronautics and Space Administration (NASA); NASA SeaWiFS and SIMBIOS Programs; and the Chesapeake Bay Program Office of the National Oceanic and Atmospheric Administration (NOAA). NR 60 TC 0 Z9 0 U1 5 U2 20 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 30 PY 2016 VL 6 AR 23773 DI 10.1038/srep23773 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH8MW UT WOS:000373048400001 PM 27026279 ER PT J AU Vaddula, BR Tantak, MP Sadana, R Gonzalez, MA Kumar, D AF Vaddula, Buchi Reddy Tantak, Mukund P. Sadana, Rachana Gonzalez, Michael A. Kumar, Dalip TI One-pot synthesis and in-vitro anticancer evaluation of 5-(2 '-indolyl)thiazoles SO SCIENTIFIC REPORTS LA English DT Article ID ANALOGS; PHYTOALEXINS; AGENTS; CAMALEXIN; BE-10988 AB A series of 5-(2'-indolyl)thiazoles were synthesized and evaluated for their cytotoxicity against selected human cancer cell lines. The reaction of thioamides 3 with 3-tosyloxypentane-2,4-dione 4 led to in situ formation of 5-acetylthiazole 5 which upon treatment with arylhydrazines 6 in polyphosphoric acid resulted in the formation of 5-(2'-indolyl)thiazoles 2. Among the synthesized 5-(2'-indolyl)thiazoles, compounds 2d-f, and 2h exhibited encouraging anticancer activity and also selectivity towards particular cell lines (IC50 = 10-30 mu M). Further studies on the SAR of compound 2e may result in good anticancer activity. C1 [Vaddula, Buchi Reddy; Gonzalez, Michael A.] US EPA, Natl Risk Management Res Lab, Sustainable Technol Div, 26 West Martin Luther King Dr,MS 483, Cincinnati, OH 45268 USA. [Tantak, Mukund P.; Kumar, Dalip] Birla Inst Technol & Sci, Dept Chem, Pilani 333031, Rajasthan, India. [Sadana, Rachana] Univ Houston Downtown, Dept Nat Sci, Houston, TX 77002 USA. RP Kumar, D (reprint author), Birla Inst Technol & Sci, Dept Chem, Pilani 333031, Rajasthan, India. EM dalipk@pilani.bits-pilani.ac.in FU University Grants Commission (UGC), New Delhi FX The authors thank the University Grants Commission (UGC), New Delhi for the financial support. B.R.V. was supported in part by an appointment to the Research Participation Program for the U.S. Environmental Protection Agency, Office of Research and Development, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the EPA. NR 27 TC 1 Z9 1 U1 1 U2 5 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 29 PY 2016 VL 6 AR 23401 DI 10.1038/srep23401 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH6QW UT WOS:000372917300001 PM 27021742 ER PT J AU Oki, NO Edwards, SW AF Oki, Noffisat O. Edwards, Stephen W. TI An integrative data mining approach to identifying adverse outcome pathway signatures SO TOXICOLOGY LA English DT Article DE Adverse outcome pathway (AOP); cpAOP; Steatosis; Glaucoma ID ARYL-HYDROCARBON RECEPTOR; FATTY LIVER-DISEASE; PRIMARY CONGENITAL GLAUCOMA; HUMAN CYP1B1 GENE; HEPATIC STEATOSIS; PEROXISOME PROLIFERATORS; CELL-DIFFERENTIATION; TRUNCATING MUTATIONS; CYTOCHROME P4501B1; LIPID-METABOLISM AB The Adverse Outcome Pathway (AOP) framework is a tool for making biological connections and summarizing key information across different levels of biological organization to connect biological perturbations at the molecular level to adverse outcomes for an individual or population. Computational approaches to explore and determine these connections can accelerate the assembly of AOPs. By leveraging the wealth of publicly available data covering chemical effects on biological systems, computationally-predicted AOPs (cpAOPs) were assembled via data mining of high-throughput screening (HTS) in vitro data, in vivo data and other disease phenotype information. Frequent Itemset Mining (FIM) was used to find associations between the gene targets of ToxCast HTS assays and disease data from Comparative Toxicogenomics Database (CTD) by using the chemicals as the common aggregators between datasets. The method was also used to map gene expression data to disease data from CTD. A cpAOP network was defined by considering genes and diseases as nodes and FIM associations as edges. This network contained 18,283 gene to disease associations for the ToxCast data and 110,253 for CTD gene expression. Two case studies show the value of the cpAOP network by extracting subnetworks focused either on fatty liver disease or the Aryl Hydrocarbon Receptor (AHR). The subnetwork surrounding fatty liver disease included many genes known to play a role in this disease. When querying the cpAOP network with the AHR gene, an interesting subnetwork including glaucoma was identified. While substantial literature exists to support the potential for AHR ligands to elicit glaucoma, it was not explicitly captured in the public annotation information in CTD. The subnetwork from this analysis suggests a cpAOP that includes changes in CYP1B1 expression, which has been previously established in the literature as a primary cause of glaucoma. These case studies highlight the value in integrating multiple data sources when defining cpAOPs for HTS data. (C) 2016 Published by Elsevier Ireland Ltd. C1 [Oki, Noffisat O.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Edwards, Stephen W.] US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Edwards, SW (reprint author), 109 TW Alexander Dr,Mail Code B305 01, Res Triangle Pk, NC 27709 USA. EM oki.noffisat@epa.gov; edwards.stephen@epa.gov FU U. S. Environmental Protection Agency FX The authors thank Carolyn Mattingly and the CTD team for invaluable assistance in working with their data. Shannon Bell originally adapted the FIM method for defining cpAOP networks from toxicogenomics data and was extremely helpful in further adapting that workflow for this study. Finally, we thank Rong-Lin Wang and Brian Chorley for constructive comments on the manuscript and Mark Nelms and Christopher Grant for reviewing the supplemental files. The information in this document has been funded wholly (or in part) by the U. S. Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 57 TC 0 Z9 0 U1 4 U2 10 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PD MAR 28 PY 2016 VL 350 BP 49 EP 61 DI 10.1016/j.tox.2016.04.004 PG 13 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA DO4GJ UT WOS:000377739500006 PM 27108252 ER PT J AU Le, CF Lehrter, JC Hu, C Obenour, DR AF Le, Chengfeng Lehrter, John C. Hu, Chuanmin Obenour, Daniel R. TI Satellite-based empirical models linking river plume dynamics with hypoxic area and volume SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article ID GULF-OF-MEXICO; CONTINENTAL-SHELF; RETROSPECTIVE ANALYSIS; ORGANIC-CARBON; VARIABILITY; DISTRIBUTIONS AB Satellite-based empirical models explaining hypoxic area and volume variation were developed for the seasonally hypoxic (O-2 < 2 mg L-1) northern Gulf of Mexico adjacent to the Mississippi River. Annual variations in midsummer hypoxic area and volume were related to Moderate Resolution Imaging Spectroradiometer-derived monthly estimates of river plume area (km(2)) and average, inner shelf chlorophyll a concentration (Chl a, mg m(-3)). River plume area in June was negatively related with midsummer hypoxic area (km(2)) and volume (km(3)), while July inner shelf Chl a was positively related to hypoxic area and volume. Multiple regression models using river plume area and Chl a as independent variables accounted for most of the variability in hypoxic area (R-2=0.92) or volume (R-2=0.89). These models explain more variation in hypoxic area than models using Mississippi River nutrient loads as independent variables. The results here also support a hypothesis that confinement of the river plume to the inner shelf is an important mechanism controlling hypoxia area and volume in this region. C1 [Le, Chengfeng] Zhejiang Univ, Ocean Coll, Hangzhou 310003, Zhejiang, Peoples R China. [Le, Chengfeng] US EPA, ORISE, Gulf Ecol Div, Gulf Breeze, FL USA. [Lehrter, John C.] US EPA, Gulf Ecol Div, Gulf Breeze, FL USA. [Hu, Chuanmin] Univ S Florida, Coll Marine Sci, St Petersburg, FL 33701 USA. [Obenour, Daniel R.] N Carolina State Univ, Dept Civil Construct & Environm Engn, Raleigh, NC 27695 USA. RP Le, CF (reprint author), Zhejiang Univ, Ocean Coll, Hangzhou 310003, Zhejiang, Peoples R China.; Le, CF (reprint author), US EPA, ORISE, Gulf Ecol Div, Gulf Breeze, FL USA. EM chengfengle@zju.edu.cn FU U.S. EPA Office of Research and Development, an Oak Ridge institute for the Science and Education (ORISE) appointment for Chengfeng Le to the EPA Research Participation Program FX This work was partly supported by the U.S. EPA Office of Research and Development, by an Oak Ridge institute for the Science and Education (ORISE) appointment for Chengfeng Le to the EPA Research Participation Program. We thank Rochelle Labiosa for a helpful review of an earlier draft of this manuscript and the two anonymous reviewers for their suggestions to improve this manuscript. NR 24 TC 1 Z9 1 U1 9 U2 12 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 EI 1944-8007 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD MAR 28 PY 2016 VL 43 IS 6 BP 2693 EP 2699 DI 10.1002/2015GL067521 PG 7 WC Geosciences, Multidisciplinary SC Geology GA DK1TM UT WOS:000374697200044 ER PT J AU Farraj, AK Malik, F Haykal-Coates, N Walsh, L Winsett, D Terrell, D Thompson, LC Cascio, WE Hazari, MS AF Farraj, Aimen K. Malik, Fatiha Haykal-Coates, Najwa Walsh, Leon Winsett, Darrell Terrell, Dock Thompson, Leslie C. Cascio, Wayne E. Hazari, Mehdi S. TI Morning NO2 exposure sensitizes hypertensive rats to the cardiovascular effects of same day O-3 exposure in the afternoon SO INHALATION TOXICOLOGY LA English DT Article DE ozone; Air pollution, blood pressure; nitrogen dioxide; sequential exposure; cardiovascular; electrocardiogram ID HEART-RATE-VARIABILITY; AMBIENT PARTICULATE MATTER; DIESEL EXHAUST INHALATION; HEALTHY-HUMAN VOLUNTEERS; FAILURE-PRONE RATS; AIR-POLLUTION; BLOOD-PRESSURE; NITROGEN-DIOXIDE; OZONE COEXPOSURE; CARDIAC-OUTPUT AB Context: Within urban air sheds, specific ambient air pollutants typically peak at predictable times throughout the day. For example, in environments dominated by mobile sources, peak nitrogen dioxide (NO2) levels coincide with morning and afternoon rush hours, while peak levels of ozone (O-3), occur in the afternoon. Objective: Given that exposure to a single pollutant might sensitize the cardiopulmonary system to the effects of a subsequent exposure to a second pollutant, we hypothesized that a morning exposure to NO2 will exaggerate the cardiovascular effects of an afternoon O-3 exposure in rats. Materials and methods: Rats were divided into four groups that were each exposed for 3 h in the morning (m) and 3 h in the afternoon (a) on the same day: (1) m-Air/a-Air, (2) m-Air/a-O-3 (0.3 ppm), (3) m-NO2 (0.5 ppm)/a-Air and (4) m-NO2/a-O-3. Implanted telemetry devices recorded blood pressure and electrocardiographic data. Sensitivity to the arrhythmogenic agent aconitine was measured in a separate cohort. Results: Only m-NO2/a-O-3-exposed rats had significant changes in electrophysiological, mechanical and autonomic parameters. These included decreased heart rate and increased PR and QT(c) intervals and increased heart rate variability, suggesting increased parasympathetic tone. In addition, only m-NO2/a-O-3 exposure decreased systolic and diastolic blood pressures and increased pulse pressure and QA interval, suggesting decreased cardiac contractility. Discussion and conclusion: The findings indicate that initial exposure to NO2 sensitized rats to the cardiovascular effects of O-3 and may provide insight into the epidemiological data linking adverse cardiovascular outcomes with exposures to low concentrations of O-3. C1 [Farraj, Aimen K.; Malik, Fatiha; Haykal-Coates, Najwa; Walsh, Leon; Winsett, Darrell; Terrell, Dock; Thompson, Leslie C.; Cascio, Wayne E.; Hazari, Mehdi S.] US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Farraj, AK (reprint author), US EPA, Environm Publ Hlth Div, Mail Code B105-02, Res Triangle Pk, NC 27711 USA. EM farraj.aimen@epa.gov NR 49 TC 0 Z9 0 U1 0 U2 1 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0895-8378 EI 1091-7691 J9 INHAL TOXICOL JI Inhal. Toxicol. PD MAR 20 PY 2016 VL 28 IS 4 BP 170 EP 179 DI 10.3109/08958378.2016.1148088 PG 10 WC Toxicology SC Toxicology GA DG9QV UT WOS:000372419800003 PM 26986952 ER PT J AU Banzhaf, HS Burtraw, D Criscimagna, SC Cosby, BJ Evans, DA Krupnick, AJ Siikamaki, JV AF Banzhaf, H. Spencer Burtraw, Dallas Criscimagna, Susie Chung Cosby, Bernard J. Evans, David A. Krupnick, Alan J. Siikamaeki, Juha V. TI Policy Analysis: Valuation of Ecosystem Services in the Southern Appalachian Mountains SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID EPISODIC ACIDIFICATION; NORTH-AMERICA; FOREST HEALTH; SMALL STREAMS; RED SPRUCE; ACID-RAIN; CONSERVATION; BIODIVERSITY; BENEFITS; CLIMATE AB This study estimates the economic value of an increase in ecosystem services attributable to the reduced acidification expected from more stringent air pollution policy. By integrating a detailed biogeochemical model that projects future ecological recovery with economic methods that measure preferences for specific ecological improvements, we estimate the economic value of ecological benefits from new air pollution policies in the Southern Appalachian ecosystem. Our results indicate that these policies generate aggregate benefits of about $3.7 billion, or about $16 per year per household in the region. The study provides currently missing information about the ecological benefits from air pollution policies that is needed to evaluate such policies comprehensively. More broadly, the study also illustrates how integrated biogeochemical and economic assessments relative benefits of different policy options that vary by scale and across ecosystem attributes. C1 [Banzhaf, H. Spencer] Georgia State Univ, Dept Econ, 14 Marietta St NW, Atlanta, GA 30303 USA. [Burtraw, Dallas; Krupnick, Alan J.; Siikamaeki, Juha V.] Resources Future Inc, 1616 P St NW, Washington, DC 20036 USA. [Criscimagna, Susie Chung] Eden Housing, Resources Future, 22645 Grand St, Hayward, CA 94541 USA. [Cosby, Bernard J.] Univ Virginia, Dept Environm Sci, 291 McCormick Rd, Charlottesville, VA 22903 USA. [Evans, David A.] US EPA, 1200 Penn Ave NW, Washington, DC 20460 USA. RP Burtraw, D (reprint author), Resources Future Inc, 1616 P St NW, Washington, DC 20036 USA. EM burtraw@rff.org RI Cosby, Bernard/B-5653-2012 FU US Environmental Protection Agency (EPA) grants [83242201, 83358801]; National Science Foundation grant [0720101] FX The work was supported by US Environmental Protection Agency (EPA) grants 83242201 and 83358801 and National Science Foundation grant 0720101. It has not been subject to formal EPA review and does not necessarily reflect the views of the Agency, and no official endorsement should be inferred. We appreciate the collaboration of Knowledge Networks (now GfK) as well as Anna Mische John, Kristen McCormack, Samantha Sekar, and Charles Driscoll. NR 49 TC 1 Z9 1 U1 3 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD MAR 15 PY 2016 VL 50 IS 6 BP 2830 EP 2836 DI 10.1021/acs.est.5b03829 PG 7 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DG9GZ UT WOS:000372392100010 PM 26871484 ER PT J AU Jasper, MN Martin, SA Oshiro, WM Ford, J Bushnell, PJ El-Masri, H AF Jasper, Micah N. Martin, Sheppard A. Oshiro, Wendy M. Ford, Jermaine Bushnell, Philip J. El-Masri, Hisham TI Application of Biologically Based Lumping To Investigate the Toxicokinetic Interactions of a Complex Gasoline Mixture SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID VOLATILE ORGANIC-COMPOUNDS; PHARMACOKINETIC MODELS; PARTITION-COEFFICIENTS; CHEMICAL-MIXTURES; METABOLIC INTERACTIONS; RAT; ETHANOL; INHALATION; HYDROCARBONS; COMPONENTS AB People are often exposed to complex mixtures of environmental chemicals such as gasoline) tobacco smoke, water contaminants, or food additives. We developed an approach that applies chemical lumping methods to complex mixtures, in this case gasoline, based on biologically relevant parameters used in physiologically based pharmacokinetic (PBPK) modeling. Inhalation exposures were performed with rats to evaluate the performance of our PBPK. model and chemical lumping method. There were 109 chemicals identified and quantified in the vapor in the chamber. The time-course toxicokinetic profiles of. 10 target chemicals were also determined from blood samples collected during and following the in viva experiments. A general PBPK model was used to compare the experimental data to the simulated values of blood concentration for 10 target chemicals with various numbers of lumps, iteratively increasing from 0 to 99. Large reductions in simulation error were gained by incorporating enzymatic chemical interactions; in comparison to simulating the individual chemicals separately. The error was further reduced by lumping the 99 nontarget chemicals. The same biologically based lumping approach can be used to simplify any complex mixture with tens, hundreds, or, thousands of constituents. C1 [Jasper, Micah N.; Martin, Sheppard A.; Oshiro, Wendy M.; Ford, Jermaine; Bushnell, Philip J.; El-Masri, Hisham] US EPA, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27709 USA. RP El-Masri, H (reprint author), US EPA, NHEERL ISTD SBB, 109 TW Alexander Dr,B105-03, Res Triangle Pk, NC 27709 USA. EM el-masri.hisham@epa.gov NR 31 TC 1 Z9 1 U1 2 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD MAR 15 PY 2016 VL 50 IS 6 BP 3231 EP 3238 DI 10.1021/acs.est.5b05648 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DG9GZ UT WOS:000372392100053 PM 26889718 ER PT J AU Salam, DA Suidan, MT Venosa, AD AF Salam, Darine A. Suidan, Makram T. Venosa, Albert D. TI Biodegradation and toxicity of vegetable oils in contaminated aquatic environments: Effect of antioxidants and oil composition SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Aquatic pollution; Vegetable oil spills; Bioremediation; Toxic biodegradation intermediates; Oil polymerization; Autoxidation products ID SALT-MARSH SEDIMENTS; ANAEROBIC BIODEGRADATION; AEROBIC BIODEGRADATION; FATTY-ACIDS; SPILLS; DEGRADATION; SUNFLOWER; TRIACYLGLYCEROLS; CONSEQUENCES; SLUDGE AB Antioxidants may affect the oxidative rate of vegetable oils determining their fate and impact in contaminated aquatic media. In previous studies, we demonstrated the effectiveness of butylated hydroxytoluene (BHT), one of the most used antioxidants in edible oils, in enhancing the biodegradation of glyceryl trilinoleate, a pure triacylglycerol of cis, cis-9,12-octadecadienoic acid (C18:2 delta), through retarding its oxidative polymerization relatively to the oil with no added antioxidant. In this study, the effect of BHT on the biodegradation and toxicity of purified canola oil, a mixed-acid triacylglycerol with high C18:1 content, was investigated in respirometric microcosms and by use of the Microtox (R) assay. Investigations were carried out in the absence and presence (200 mg kg(-1)) of the antioxidant, and at an oil loading of 0.31 L m(-2) (333 gal acre(-1)). Substantial oil mineralization was achieved after 16 weeks of incubation (> 77%) and was not significantly different (p > 0.05) between the two BHT treatments, demonstrating an important role of the oil fatty acid composition in determining the potency of antioxidants and, consequently, the fate of spilled vegetable oils. Furthermore, for both treatments, toxicity was measured at early stages of the experiments and disappeared at a later stage of incubation. The observed transient toxicity was associated with the combined effect of toxic biodegradation intermediates and autoxidation products. These results were supported by the gradual disappearance of BHT in the microcosms initially supplemented with the antioxidant, reaching negligible amounts after only 2 weeks of incubation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Salam, Darine A.; Suidan, Makram T.] Amer Univ Beirut, Fac Engn & Architecture, Dept Civil & Environm Engn, POB 11-0236, Beirut, Lebanon. [Venosa, Albert D.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. RP Salam, DA (reprint author), Univ Beirut, Beirut Fac Engn & Architecture, Bechtel Engn Bldg 427,POB 11-0236, Beirut 11072020, Lebanon. EM ds40@aub.edu.lb FU National Risk Management Research Laboratory of the US Environmental Protection Agency [EPC-11-006] FX This research was funded by the National Risk Management Research Laboratory of the US Environmental Protection Agency (EPC-11-006). NR 38 TC 1 Z9 1 U1 3 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD MAR 15 PY 2016 VL 547 BP 95 EP 103 DI 10.1016/j.scitotenv.2015.12.138 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DD0ST UT WOS:000369630600010 PM 26780134 ER PT J AU Kaminski, MD Lee, SD Magnuson, M AF Kaminski, Michael D. Lee, Sang Don Magnuson, Matthew TI Wide-area decontamination in an urban environment after radiological dispersion: A review and perspectives SO JOURNAL OF HAZARDOUS MATERIALS LA English DT Review DE Decontamination; Remediation; Radionuclide dispersal device; Nuclear fallout; Radioactive contamination ID COATINGS; SURFACES; SORPTION AB Nuclear or radiological terrorism in the form of uncontrolled radioactive contamination presents a unique challenge in the field of nuclear decontamination. Potential targets require an immediate decontamination response, or mitigation plan to limit the social and economic impact. To date, experience with urban decontamination of building materials - specifically hard, porous, external surfaces - is limited to nuclear weapon fallout and nuclear reactor accidents. Methods are lacking for performing wide-area decontamination in an urban environment so that in all release scenarios the area may be re -occupied without evaluation and/or restriction. Also lacking is experience in developing mitigation strategies, that is, methods of mitigating contamination and its resultant radiation dose in key areas during the immediate aftermath of an event and after lifesaving operations. To date, the tremendous strategy development effort primarily by the European community has focused on the recovery phase, which extends years beyond the release event. In this review, we summarize the methods and data collected over the past 70 years in the field of hard, external surface decontamination of radionuclide contaminations, with emphasis on methods suitable for response to radiological dispersal devices and their potentially unique physico-chemical characteristics. This review concludes that although a tremendous amount of work has been completed primarily by the European Community (EU) and the United Kingdom (UK), the few studies existing on each technique permit only very preliminary estimates of decontamination factors for various building materials and methods and extrapolation of those values for use in environments outside the EU and UK. This data shortage prevents us from developing an effective and detailed mitigation response plan and remediation effort. Perhaps most importantly, while the data available does include valuable information on the practical aspects of performing the various remediation methods including costs, coverage rates, manpower, pitfalls, etc., it lacks the details on lessons learned, best practices, and standard procedures, for instance, that would be required to develop a mitigation strategy. While the urban decontamination problem is difficult and there is much more research to do, the existing literature provides a framework for a response plan. Using this framework, in conjunction with computer modeling and relevant data collection, can lead to development of appropriate plans and exercises that would permit development of a mitigation and remediation response. (C) 2015 Elsevier B.V. All rights reserved. C1 [Kaminski, Michael D.] Argonne Natl Lab, Nucl Engn Div, Nucl Decontaminat & Separat, Argonne, IL 60439 USA. [Lee, Sang Don; Magnuson, Matthew] US EPA, Off Res & Dev, Natl Homeland Secur Res Ctr, Washington, DC USA. RP Kaminski, MD (reprint author), Argonne Natl Lab, Nucl Engn Div, Nucl Decontaminat & Separat, Argonne, IL 60439 USA. EM Kaminski@anl.gov FU United States Department of Homeland Security/Science and Technology Directorate; U.S. Environmental Protection Agency/National Homeland Security Research Center; U.S. Department of Energy Office of Science laboratory [DE-AC02-06CH11357] FX The authors thank J. Harmon and J. T. Bursey for editing the document. This work was supported by the United States Department of Homeland Security/Science and Technology Directorate in collaboration with the U.S. Environmental Protection Agency/National Homeland Security Research Center through an interagency agreement. It has been subject to an administrative review but does not necessarily reflect the views of either Agency. The submitted manuscript has been created by UChicago Argonne, LLC, Operator of Argonne National Laboratory ("Argonne"). Argonne, a U.S. Department of Energy Office of Science laboratory, is operated under Contract No. DE-AC02-06CH11357. The U.S. Government retains for itself, and others acting on its behalf, a paid-up nonexclusive, irrevocable worldwide license in said article to reproduce, prepare derivative works, distribute copies to the public, and perform publicly and display publicly, by or on behalf of the Government. NR 100 TC 3 Z9 3 U1 3 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3894 EI 1873-3336 J9 J HAZARD MATER JI J. Hazard. Mater. PD MAR 15 PY 2016 VL 305 BP 67 EP 86 DI 10.1016/j.jhazmat.2015.11.014 PG 20 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DC8DZ UT WOS:000369451400009 PM 26642448 ER PT J AU Sawyer, ME Evans, MV Wilson, CA Beesley, LJ Leon, LS Eklund, CR Croom, EL Pegram, RA AF Sawyer, Megan E. Evans, Marina V. Wilson, Charles A. Beesley, Lauren J. Leon, Lider S. Eklund, Chris R. Croom, Edward L. Pegram, Rex A. TI Development of a human physiologically based pharmacokinetic (PBPK) model for dermal permeability for lindane SO TOXICOLOGY LETTERS LA English DT Article DE PBPK; Lindane; Dermal permeability ID IN-VITRO; PERCUTANEOUS-ABSORPTION; SKIN DISTRIBUTION; EXPOSURE; PREDICTION; PERMEATION; CHEMICALS; BLOOD; VIVO AB Lindane is a neurotoxicant used for the treatment of lice and scabies present on human skin. Due to its pharmaceutical application, an extensive pharmacokinetic database exists in humans. Mathematical diffusion models allow for calculation of lindane skin permeability coefficients using human kinetic data obtained from in vitro and in vivo experimentation as well as a default compound -specific calculation based on physicochemical characteristics used in the absence of kinetic data. A dermal model was developed to describe lindane diffusion into the skin, where the skin compartment consisted of homogeneous dermal tissue. This study utilized Fick's law of diffusion along with chemical binding to protein and lipids to determine appropriate dermal absorption parameters which were then incorporated into a physiologically based pharmacokinetic (PBPK) model to describe in vivo kinetics. The estimation of permeability coefficients using chemical binding in combination with in vivo data demonstrates the advantages of combining physiochemical properties with a PBPK model to predict dermal absorption. (C) 2016 Elsevier Ireland Ltd. All rights reserved. C1 [Sawyer, Megan E.] N Carolina State Univ, Dept Math, Campus Box 8205, Raleigh, NC 27695 USA. [Evans, Marina V.; Eklund, Chris R.; Croom, Edward L.; Pegram, Rex A.] US Environm Protect Agcy, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27709 USA. [Wilson, Charles A.; Beesley, Lauren J.; Leon, Lider S.] N Carolina State Univ, Dept Math, Raleigh, NC 27695 USA. RP Sawyer, ME (reprint author), Southern New Hampshire Univ, Dept Math, Manchester, NH 03106 USA. EM m.sawyer@snhu.edu FU NSF [DMS-1063010]; NSA [H9823-10-1-0252]; Southern New Hampshire University FX The authors would like to thank North Carolina State University and the US EPA for making this work possible. Dr. Michael F Hughes from the US EPA provided insightful comments that helped research progress. Many thanks to Dr. Paul A Lehman for unique insights into dermal experimentation. We also want to thank the following reviewers for providing insightful comments: Drs. Jane C Caldwell, Hisham A El-Masri, and Rogelio Tornero-Velez, as well as the anonymous reviewers for their helpful comments. The Research Experience for Undergraduates (REU) work was possible due to NSF grant number DMS-1063010 and NSA grant number H9823-10-1-0252. MES was partially supported through a Southern New Hampshire University 2015 Summer Research Grant. NR 21 TC 1 Z9 1 U1 5 U2 15 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0378-4274 EI 1879-3169 J9 TOXICOL LETT JI Toxicol. Lett. PD MAR 14 PY 2016 VL 245 BP 106 EP 109 DI 10.1016/j.toxlet.2016.01.008 PG 4 WC Toxicology SC Toxicology GA DD5CU UT WOS:000369941000013 PM 26794662 ER PT J AU Lakey, PSJ George, IJ Baeza-Romero, MT Whalley, LK Heard, DE AF Lakey, Pascale S. J. George, Ingrid J. Baeza-Romero, Maria T. Whalley, Lisa K. Heard, Dwayne E. TI Organics Substantially Reduce HO2 Uptake onto Aerosols Containing Transition Metal ions SO JOURNAL OF PHYSICAL CHEMISTRY A LA English DT Article ID MARINE BOUNDARY-LAYER; UPTAKE COEFFICIENTS; PARTICLES; CHEMISTRY; RADICALS; OH; TROPOSPHERE; MODEL; ACID; KINETICS AB A HO2 mass accommodation coefficient of alpha = 0.23 +/- 0.07 was measured onto submicron copper(II)-doped ammonium sulfate aerosols: at a relative humidity of 60 +/- 3%, at 293 +/- 2 K and at an initial HO2 concentration of. similar to 1 X 10(9) molecules cm(-3) by using an aerosol flow tube coupled to a sensitive fluorescence assay by gas expansion (FAGS) HO2 detection system. The effect upon the HO2 uptake coefficient gamma of adding different organic species (malonic acid, citric acid, 1,2-diaminoethane, tartronic acid, ethylenediaminetetraacetic acid (EDTA), and oxalic acid) into the copper(II)-doped aerosols was investigated. The HO2 uptake coefficient decreased steadily from the mass accommodation value to gamma = 0.008 +/- 0.009 when EDTA was added in a one-to-one molar ratio with the copper(II) ions, and. to gamma = 0.003 +/- 0.004 when oxalic acid was added into the aerosol in a ten-to-one molar ratio with the copper(II). EDTA binds strongly to copper(II) ions, potentially making them unavailable for catalytic destruction of HO2 and could also be acting as a surfactant or changing the viscosity of the aerosol. The addition of oxalic acid to the aerosol potentially forms low-volatility copper-oxalate complexes that reduce the uptake of HO2 either by changing the viscosity of the aerosol or by causing precipitation but of the aerosol forming a coating. It is likely that there is a high enough oxalate to copper(II) ion ratio in many types of atmospheric aerosols to decrease the HO2 uptake coefficient. No observable change in the HO2 uptake coefficient was measured when the other organic species (malonic acid, citric acid, 1,2-diaminoethane, and tartronic acid) were added in a ten-to-one molar ratio with the copper(II) ions. C1 [Lakey, Pascale S. J.; Whalley, Lisa K.; Heard, Dwayne E.] Univ Leeds, Sch Chem, Woodhouse Lane, Leeds LS16 6QB, W Yorkshire, England. [Whalley, Lisa K.; Heard, Dwayne E.] Univ Leeds, Natl Ctr Atmospher Chem, Woodhouse Lane, Leeds LS16 6QB, W Yorkshire, England. [Baeza-Romero, Maria T.] Univ Castilla la Mancha, Escuela Ingn Ind Toledo, Ave Carlos III S-N Real Fabrica Armas, Toledo 45071, Spain. [George, Ingrid J.] US EPA, Natl Risk Management Res Lab, TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Lakey, Pascale S. J.] Care of Matthews N, Max Planck Inst Chem, Hahn Meitner Weg 1, D-55128 Mainz, Germany. RP Heard, DE (reprint author), Univ Leeds, Sch Chem, Woodhouse Lane, Leeds LS16 6QB, W Yorkshire, England.; Heard, DE (reprint author), Univ Leeds, Natl Ctr Atmospher Chem, Woodhouse Lane, Leeds LS16 6QB, W Yorkshire, England. EM d.e.heard@leeds.ac.uk OI Heard, Dwayne/0000-0002-0357-6238 FU National Environment Research Council [NE/F020651/1]; NERC FX This work was supported by the National Environment Research Council under grant NE/F020651/1. P.S.J.L. is grateful to NERC for the award of a studentship. L.K.W. and D.E.H. are also grateful to the National Centre for Atmospheric Science, which is funded by NERC, for ongoing support. NR 54 TC 1 Z9 1 U1 3 U2 22 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1089-5639 J9 J PHYS CHEM A JI J. Phys. Chem. A PD MAR 10 PY 2016 VL 120 IS 9 BP 1421 EP 1430 DI 10.1021/acs.jpca.5b06316 PG 10 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA DG4KX UT WOS:000372042200010 PM 26484935 ER PT J AU Oshida, K Vasani, N Waxman, DJ Corton, JC AF Oshida, Keiyu Vasani, Naresh Waxman, David J. Corton, J. Christopher TI Disruption of STAT5b-Regulated Sexual Dimorphism of the Liver Transcriptome by Diverse Factors Is a Common Event SO PLOS ONE LA English DT Article ID GROWTH-HORMONE-SECRETION; FEMALE MOUSE-LIVER; GENE-EXPRESSION; SIGNAL TRANSDUCER; GLUCOCORTICOID-RECEPTOR; NUCLEAR FACTOR-4-ALPHA; MICROARRAY ANALYSIS; BODY GROWTH; CROSS-TALK; GH ACTION AB Signal transducer and activator of transcription 5b (STAT5b) is a growth hormone (GH)-activated transcription factor and a master regulator of sexually dimorphic gene expression in the liver. Disruption of the GH hypothalamo-pituitary-liver axis controlling STAT5b activation can lead to metabolic dysregulation, steatosis, and liver cancer. Computational approaches were developed to identify factors that disrupt STAT5b function in a mouse liver gene expression compendium. A biomarker comprised of 144 STAT5b-dependent genes was derived using comparisons between wild-type male and wild-type female mice and between STAT5b-null and wild-type mice. Correlations between the STAT5b biomarker gene set and a test set comprised of expression datasets (biosets) with known effects on STAT5b function were evaluated using a rank-based test (the Running Fisher algorithm). Using a similarity p-value <= 10(-4), the test achieved a balanced accuracy of 99% and 97% for detection of STAT5b activation or STAT5b suppression, respectively. The STAT5b biomarker gene set was then used to identify factors that activate (masculinize) or suppress (feminize) STAT5b function in an annotated mouse liver and primary hepatocyte gene expression compendium of similar to 1,850 datasets. Disruption of GH-regulated STAT5b is a common phenomenon in liver in vivo, with 5% and 29% of the male datasets, and 11% and 13% of the female datasets, associated with masculinization or feminization, respectively. As expected, liver STAT5b activation/masculinization occurred at puberty and suppression/feminization occurred during aging and in mutant mice with defects in GH signaling. A total of 70 genes were identified that have effects on STAT5b activation in genetic models in which the gene was inactivated or overexpressed. Other factors that affected liver STAT5b function were shown to include fasting, caloric restriction and infections. Together, these findings identify diverse factors that perturb the hypothalamo-pituitary-liver GH axis and disrupt GH-dependent STAT5b activation in mouse liver. C1 [Oshida, Keiyu; Vasani, Naresh; Corton, J. Christopher] US EPA, Integrated Syst Toxicol Div, NHEERL ORD, Res Triangle Pk, NC 27711 USA. [Waxman, David J.] Boston Univ, Dept Biol, Div Cell & Mol Biol, Boston, MA 02215 USA. [Waxman, David J.] Boston Univ, Bioinformat Program, Boston, MA 02215 USA. [Oshida, Keiyu] Toray Industries Ltd, Kamakura, Kanagawa, Japan. RP Corton, JC (reprint author), US EPA, Integrated Syst Toxicol Div, NHEERL ORD, Res Triangle Pk, NC 27711 USA. EM corton.chris@epa.gov OI Waxman, David/0000-0001-7982-9206 FU U.S. Environmental Protection Agency; NIH [DK33765] FX The information in this document has been funded in part by the U.S. Environmental Protection Agency, and by NIH grant DK33765 (to DJW). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 78 TC 0 Z9 0 U1 0 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 9 PY 2016 VL 11 IS 3 AR e0148308 DI 10.1371/journal.pone.0148308 PG 27 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG3TF UT WOS:000371992300002 PM 26959975 ER PT J AU Oshida, K Waxman, DJ Corton, JC AF Oshida, Keiyu Waxman, David J. Corton, J. Christopher TI Chemical and Hormonal Effects on STAT5b-Dependent Sexual Dimorphism of the Liver Transcriptome SO PLOS ONE LA English DT Article ID PROLIFERATOR-ACTIVATED RECEPTOR; GROWTH-HORMONE; GENE-EXPRESSION; SIGNAL TRANSDUCER; RAT-LIVER; NONALCOHOLIC STEATOHEPATITIS; GLUCOCORTICOID-RECEPTOR; MICROARRAY ANALYSIS; HEPATIC STEATOSIS; THYROID-HORMONE AB The growth hormone (GH)-activated transcription factor signal transducer and activator of transcription 5b (STAT5b) is a key regulator of sexually dimorphic gene expression in the liver. Suppression of hepatic STAT5b signaling is associated with lipid metabolic dysfunction leading to steatosis and liver cancer. In the companion publication, a STAT5b biomarker gene set was identified and used in a rank-based test to predict both increases and decreases in liver STAT5b activation status/function with high (>= 97%) accuracy. Here, this computational approach was used to identify chemicals and hormones that activate (masculinize) or suppress (feminize) STAT5b function in a large, annotated mouse liver and primary hepatocyte gene expression compendium. Exposure to dihydrotestosterone and thyroid hormone caused liver masculinization, whereas glucocorticoids, fibroblast growth factor 15, and angiotensin II caused liver feminization. In mouse models of diabetes and obesity, liver feminization was consistently observed and was at least partially reversed by leptin or resveratrol exposure. Chemical-induced feminization of male mouse liver gene expression profiles was a relatively frequent phenomenon: of 156 gene expression biosets from chemically-treated male mice, 29% showed feminization of liver STAT5b function, while < 1% showed masculinization. Most (93%) of the biosets that exhibited feminization of male liver were also associated with activation of one or more xenobiotic-responsive receptors, most commonly constitutive activated receptor (CAR) or peroxisome proliferator-activated receptor alpha (PPARa). Feminization was consistently associated with increased expression of peroxisome proliferator-activated receptor gamma (Pparg) but not other lipogenic transcription factors linked to steatosis. GH-activated STAT5b signaling in mouse liver is thus commonly altered by diverse chemicals, and provides a linkage between chemical exposure and dysregulated gene expression associated with adverse effects on the liver. C1 [Oshida, Keiyu; Corton, J. Christopher] US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Waxman, David J.] Boston Univ, Div Cell & Mol Biol, Dept Biol & Bioinformat Program, Boston, MA 02215 USA. [Oshida, Keiyu] Toray Industries Ltd, Kamakura, Kanagawa, Japan. RP Corton, JC (reprint author), US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. EM corton.chris@epa.gov OI Waxman, David/0000-0001-7982-9206 FU U.S. Environmental Protection Agency; National Institutes of Health (NIH) [ES024421, DK33765] FX The information in this document has been funded in part by the U.S. Environmental Protection Agency, and by National Institutes of Health (NIH) grants ES024421 and DK33765 (to DJW). NR 79 TC 2 Z9 2 U1 0 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 9 PY 2016 VL 11 IS 3 AR e0150284 DI 10.1371/journal.pone.0150284 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG3TF UT WOS:000371992300042 PM 26959237 ER PT J AU Vandenberg, LN Gerona, RR Kannan, K Taylor, JA van Breemen, RB Dickenson, CA Liao, CY Yuan, Y Newbold, RR Padmanabhan, V vom Saal, FS Woodruff, TJ AF Vandenberg, Laura N. Gerona, Roy R. Kannan, Kurunthachalam Taylor, Julia A. van Breemen, Richard B. Dickenson, Carrie A. Liao, Chunyang Yuan, Yang Newbold, Retha R. Padmanabhan, Vasantha vom Saal, Frederick S. Woodruff, Tracey J. TI A round robin approach to the analysis of bisphenol a (BPA) in human blood samples (vol 13, 25, 2014) SO ENVIRONMENTAL HEALTH LA English DT Correction C1 [Vandenberg, Laura N.] Univ Massachusetts, Sch Publ Hlth, Div Environm Hlth Sci, Amherst, MA 01003 USA. [Gerona, Roy R.] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA. [Kannan, Kurunthachalam] SUNY Albany, New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12222 USA. [Taylor, Julia A.; vom Saal, Frederick S.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [van Breemen, Richard B.; Yuan, Yang] Univ Illinois, Coll Pharm, Chicago, IL USA. [Dickenson, Carrie A.; Woodruff, Tracey J.] Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, Program Reprod Hlth & Environm, San Francisco, CA 94143 USA. [Liao, Chunyang] NY State Dept Publ Hlth, Wadsworth Ctr, Albany, NY USA. [Newbold, Retha R.] Natl Inst Environm Hlth Sci, Res Triangle Pk, NC USA. [Padmanabhan, Vasantha] Univ Michigan, Dept Pediat, Ann Arbor, MI 48109 USA. [Padmanabhan, Vasantha] Univ Michigan, Reprod Sci Program, Ann Arbor, MI 48109 USA. RP Woodruff, TJ (reprint author), Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, Program Reprod Hlth & Environm, San Francisco, CA 94143 USA. EM woodrufft@obgyn.ucsf.edu FU NICHD NIH HHS [R01 HD021341]; NIDDK NIH HHS [P30 DK020572]; NIEHS NIH HHS [R01 ES017005, R21 ES017763, P01 ES022841, P20 ES018135, R01 ES013527] NR 1 TC 0 Z9 0 U1 2 U2 4 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1476-069X J9 ENVIRON HEALTH-GLOB JI Environ. Health PD MAR 8 PY 2016 VL 15 AR 43 DI 10.1186/s12940-016-0126-z PG 1 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DF6JT UT WOS:000371462500001 PM 26956378 ER PT J AU Vesper, S Choi, H Perzanowski, MS Acosta, LM Divjan, A Bolanos-Rosero, B Rivera-Mariani, F Chew, GL AF Vesper, Stephen Choi, Hyunok Perzanowski, Matthew S. Acosta, Luis M. Divjan, Adnan Bolanos-Rosero, Benjamin Rivera-Mariani, Felix Chew, Ginger L. TI Mold populations and dust mite allergen concentrations in house dust samples from across Puerto Rico SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH LA English DT Article DE mold; dust; mites; ERMI; relative humidity ID RELATIVE MOLDINESS INDEX; NEW-YORK; ASTHMA; EXPOSURE; ASPERGILLUS; PREVALENCE; CHILDREN; FUNGI; IGE AB Lifetime childhood asthma prevalence (LCAP) percentages in Puerto Rico Health Regions (HR) are substantially higher in northeastern vs. southwestern HR. Higher average relative humidity in the northeast might promote mold and mite exposures and possibly asthma prevalence. To test this hypothesis, mold contamination, Environmental Relative Moldiness Index (ERMI) values were measured in floor dust (n=26) and dust mite allergen concentrations in bed dust (n=14). For this analysis, the eight HR were divided into those with LCAP >30% (n=3) and <30% (n=5). The average ERMI value was significantly greater (Wilcoxon Rank Sum, p<0.001) in high than in low LCAP HR (14.5 vs. 9.3). The dust mite antigens Der p 1, Der f 1, and Blo t 5 were detected in 90% of bed samples, but the concentrations were not significantly different in high vs. low LCAP HR. Mold exposures might partially explain the differences in LCAP HR in Puerto Rico. C1 [Vesper, Stephen; Chew, Ginger L.] US EPA, Cincinnati, OH 45268 USA. [Choi, Hyunok] Univ Albany, Sch Publ Hlth, Dept Environm Hlth Sci, Rensselaer, NY USA. [Perzanowski, Matthew S.; Acosta, Luis M.; Divjan, Adnan; Chew, Ginger L.] Columbia Univ, Dept Environm Hlth Sci, Mailman Sch Publ Hlth, New York, NY USA. [Bolanos-Rosero, Benjamin] Univ Puerto Rico, Dept Microbiol & Med Zool, San Juan, PR 00936 USA. [Rivera-Mariani, Felix] Miami Dade Coll, Hlth & Wellness Dept, Miami, FL USA. RP Chew, GL (reprint author), Columbia Univ, Dept Environm Hlth Sci, Mailman Sch Publ Hlth, New York, NY USA. EM cg288@columbia.edu RI Rivera-Mariani, Felix/B-1578-2012 OI Rivera-Mariani, Felix/0000-0002-6671-0174 FU National Institutes of Health [P30 ES 009089, R01 ES 10922] FX This work was supported by the National Institutes of Health [grant number P30 ES 009089], [grant number R01 ES 10922]. NR 30 TC 0 Z9 0 U1 0 U2 6 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0960-3123 EI 1369-1619 J9 INT J ENVIRON HEAL R JI Int. J. Environ. Health Res. PD MAR 3 PY 2016 VL 26 IS 2 BP 198 EP 207 DI 10.1080/09603123.2015.1089531 PG 10 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DB9AA UT WOS:000368807100006 PM 26412677 ER PT J AU Eddy, EN Roman, CT AF Eddy, Elizabeth N. Roman, Charles T. TI Relationship Between Epibenthic Invertebrate Species Assemblages and Environmental Variables in Boston Harbor's Intertidal Habitat SO NORTHEASTERN NATURALIST LA English DT Article ID GRAIN-SIZE DISTRIBUTION; ORGANIC-MATTER; WAVE EXPOSURE; ROCKY SHORES; COMMUNITY STRUCTURE; MARINE HABITATS; TIDAL COMMUNITY; SURFACE-AREA; COBSCOOK BAY; PATTERNS AB The Boston Harbor Islands National Recreation Area has an extensive intertidal zone, with 47% of the area composed of mixed-coarse substrate. Given anticipated climate-change impacts such as sea-level rise and ocean warming, and other stressors associated with the urban environment, the critical ecosystem functions (i.e., species habitat, food-web support) provided by this dominant mixed-coarse habitat of Boston Harbor, and elsewhere throughout the Northeast, have been and will likely be further altered. To evaluate the present-day epibenthic invertebrate communities and to determine what environmental factors of the mixed-coarse substrate affect community structure, we used a stratified random design to sample epibenthic macroinvertebrates along with various physical and environmental variables from the intertidal zone. Epibenthic macroinvertebrate species assemblages and diversity differed significantly between wave-exposed and wave-protected sites, with higher diversity present at protected sites. We also found that environmental variables collectively explained up to 67% of the variation in species assemblages, with elevation, organic content, water content, and sediment type individually explaining up to 56%, 30%, 42%, and 33% of the variation, respectively. This study provides a baseline for long-term monitoring aimed at understanding the response of cobble and mixed-coarse intertidal communities to multiple disturbances, and a foundation to support habitat restoration or other management actions. C1 [Eddy, Elizabeth N.] Univ Rhode Isl, Grad Sch Oceanog, Narragansett, RI 02882 USA. [Roman, Charles T.] Univ Rhode Isl, Natl Pk Serv, Narragansett, RI 02882 USA. RP Eddy, EN (reprint author), US EPA, ORISE Res Participat Program, Washington, DC 20460 USA. EM eeddy@my.uri.edu FU National Park Service FX Funding for this research was provided by the National Park Service, with funds administered through the North Atlantic Coast Cooperative Ecosystem Studies Unit at the University of Rhode Island. We thank Penelope Pooler Eisenbies for contributions to the study design; Sarah Waterworth, Annie Kreider, and the Green Ambassadors for their field assistance; Sheldon Pratt and Sebastian Kvist for taxonomic guidance; John King and Danielle Cares for sediment and grain-size analysis assistance; and Candace Oviatt, Carol Thornber, Mary-Jane James, and Marc Albert for their support throughout the study. NR 76 TC 0 Z9 0 U1 17 U2 23 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1092-6194 EI 1938-5307 J9 NORTHEAST NAT JI Northeast. Nat PD MAR PY 2016 VL 23 IS 1 BP 45 EP 66 PG 22 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP0FG UT WOS:000378163600006 ER PT J AU Leidy, RA Bogan, MT Neuhaus, L Rosetti, L Carlson, SM AF Leidy, Robert A. Bogan, Michael T. Neuhaus, Linnea Rosetti, Leana Carlson, Stephanie M. TI SUMMER DIE-OFF OF WESTERN POND TURTLE (ACTINEMYS MARMORATA) ALONG AN INTERMITTENT COAST RANGE STREAM IN CENTRAL CALIFORNIA SO SOUTHWESTERN NATURALIST LA English DT Article ID TERRESTRIAL HABITAT USE AB During late summer and fall 2014, we documented western pond turtle (Actinemys marmorata) mortality, as indicated by the presence of turtle shells, along a 3.7-km reach of Coyote Creek in the Diablo Range of central California. In total, we observed 39 western pond turtle shells scattered irregularly along our study reach. Shells were found in dry reaches adjacent to or close to pools containing live turtles, as well as in or adjacent to dry pools in isolated dry reaches. Ninety percent of shells observed contained no carcass, and several shells showed evidence of predation. Though the cause of mortality is unclear, our observations confirm that western pond turtles may experience high mortality during droughts, which could result in significant population decline. The presence of live turtles in refugial pools emphasizes the importance of protecting and managing permanent pools in the face of intensified drought conditions. C1 [Leidy, Robert A.; Rosetti, Leana] US EPA, San Francisco, CA 94105 USA. [Bogan, Michael T.; Carlson, Stephanie M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Neuhaus, Linnea] Urban Creeks Council, Berkeley, CA 94720 USA. RP Leidy, RA (reprint author), US EPA, San Francisco, CA 94105 USA. EM leidy.robert@epa.gov FU Rose Hills Innovator Award at UC-Berkeley; Smith Postdoctoral Fellowship; USDA National Institute of Food and Agriculture, Animal Health project [1004229] FX We thank Henry Coe State Park for providing access to the study reach at Coyote Creek. This research was funded by the Rose Hills Innovator Award at UC-Berkeley to SMC and a Smith Postdoctoral Fellowship to MTB. This work was also supported by the USDA National Institute of Food and Agriculture, Animal Health project [1004229] to SMC. We thank S. Kelson for assistance in the field and P. L. Fiedler for review of the manuscript. NR 14 TC 0 Z9 0 U1 4 U2 4 PU SOUTHWESTERN ASSOC NATURALISTS PI SAN MARCOS PA SOUTHWEST TEXAS STATE UNIV, DEPT BIOLOGY, 601 UNIVERSITY DR, SAN MARCOS, TX 78666 USA SN 0038-4909 EI 1943-6262 J9 SOUTHWEST NAT JI Southw. Natural. PD MAR PY 2016 VL 61 IS 1 BP 71 EP 74 PG 6 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DO9DL UT WOS:000378084400014 ER PT J AU Milanovich, JR Hopton, ME AF Milanovich, Joseph R. Hopton, Matthew E. TI Stoichiometry of Excreta and Excretion Rates of a Stream-dwelling Plethodontid Salamander SO COPEIA LA English DT Article; Proceedings Paper CT 6th Conference on the Biology of Plethodontid Salamanders CY MAY 18-20, 2014 CL Univ Tulsa, Tulsa, OK HO Univ Tulsa ID HEADWATER STREAMS; LARVAL SALAMANDERS; NUTRIENT; FISH; NITROGEN; FOREST; MACROINVERTEBRATE; CONSUMERS; UREA AB Nutrient recycling by fish and amphibians can contribute significantly to ecosystem processes in freshwater ecosystems. Variation in the components that comprise excreta and the factors that control excretion rates may result in differences in the rates and ratios of nutrients excreted by these taxa. These factors can subsequently influence the ecological role of fish and amphibians in freshwater systems. We assessed the composition of excreta of larval Eurycea cirrigera in six headwater streams, and investigated the influence of body size, organismal stoichiometry, and stream nutrient concentrations on mass-specific excretion rates. Excretion components and excretion rates of E. cirrigera were compared to fish in stream and river systems. Excretion of urea comprised the majority of excreted waste in E. cirrigera. This is considerably different from freshwater fish, where the majority of excreted nitrogenous waste is in the form of NH4. Excretion of NH4, soluble reactive phosphorus (SRP), and urea by E. cirrigera differed across streams. Body size was the most important predictor of excretion rates of NH4 and the ratio of N:P in the larvae of E. cirrigera, and no other variables tested influenced the rates of excretion. These estimates reveal larval stream-dwelling plethodontids' excretion rates of inorganic nutrients (NH4 and SRP) are lower compared to fish in similar systems. However, the contributions of urea (organic forms of nitrogen) are higher. Considering many headwater streams are fishless, stream-dwelling plethodontids, such as E. cirrigera, could represent a primary method of nutrient recycling of limiting nutrients in headwater streams, particularly more labile forms of organic nitrogen. C1 [Milanovich, Joseph R.; Hopton, Matthew E.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Sustainable Technol Div,Sustainable Environm Bran, 26 West Martin Luther King Dr,MS 443, Cincinnati, OH 45268 USA. [Milanovich, Joseph R.] Loyola Univ, Dept Biol, 1050 W Sheridan Rd, Chicago, IL 60660 USA. RP Hopton, ME (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, Sustainable Technol Div,Sustainable Environm Bran, 26 West Martin Luther King Dr,MS 443, Cincinnati, OH 45268 USA. EM jmilanovich@luc.edu; hopton.matthew@epa.gov OI Hopton, Matt/0000-0001-7962-6820 NR 50 TC 0 Z9 0 U1 7 U2 10 PU AMER SOC ICHTHYOLOGISTS & HERPETOLOGISTS PI MIAMI PA MAUREEN DONNELLY, SECRETARY FLORIDA INT UNIV BIOLOGICAL SCIENCES, 11200 SW 8TH STREET, MIAMI, FL 33199 USA SN 0045-8511 EI 1938-5110 J9 COPEIA JI Copeia PD MAR PY 2016 VL 104 IS 1 BP 26 EP 34 DI 10.1643/OT-14-028 PG 9 WC Zoology SC Zoology GA DM2OK UT WOS:000376185900004 ER PT J AU Beauchamp, JD Pleil, JD Dweik, RA Herbig, J Risby, TH AF Beauchamp, Jonathan D. Pleil, Joachim D. Dweik, Raed A. Herbig, Jens Risby, Terence H. TI International Association of Breath Research 10th anniversary conference at the Schoenbrunn Palace in Vienna, Austria SO JOURNAL OF BREATH RESEARCH LA English DT Editorial Material C1 [Beauchamp, Jonathan D.] Fraunhofer Inst Proc Engn & Packaging IVV, Dept Sensory Analyt, Freising Weihenstephan, Germany. [Pleil, Joachim D.] US EPA, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC 27709 USA. [Dweik, Raed A.] Cleveland Clin, Resp Inst, Dept Pulm Med, Cleveland, OH 44195 USA. [Herbig, Jens] IONICON Analyt GesmbH, Innsbruck, Austria. [Risby, Terence H.] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Baltimore, MD 21205 USA. RP Beauchamp, JD (reprint author), Fraunhofer Inst Proc Engn & Packaging IVV, Dept Sensory Analyt, Freising Weihenstephan, Germany. EM jonathan.beauchamp@ivv.fraunhofer.de NR 10 TC 2 Z9 2 U1 0 U2 1 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1752-7155 EI 1752-7163 J9 J BREATH RES JI J. Breath Res. PD MAR PY 2016 VL 10 IS 1 AR 019001 DI 10.1088/1752-7155/10/1/019001 PG 4 WC Biochemical Research Methods; Respiratory System SC Biochemistry & Molecular Biology; Respiratory System GA DL6LC UT WOS:000375749700025 ER PT J AU Pleil, JD AF Pleil, Joachim D. TI Cellular respiration: replicating in vivo systems biology for in vitro exploration of human exposome, microbiome, and disease pathogenesis biomarkers SO JOURNAL OF BREATH RESEARCH LA English DT Editorial Material ID MASS-SPECTROMETRY; LUNG-CANCER; BREATH; TOXICOLOGY; INFECTION; RISK C1 [Pleil, Joachim D.] US EPA, Exposure Methods & Measurements Div, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Pleil, JD (reprint author), US EPA, Exposure Methods & Measurements Div, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. EM pleil.joachim@epa.gov NR 44 TC 5 Z9 5 U1 3 U2 5 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1752-7155 EI 1752-7163 J9 J BREATH RES JI J. Breath Res. PD MAR PY 2016 VL 10 IS 1 AR 010201 DI 10.1088/1752-7155/10/1/010201 PG 5 WC Biochemical Research Methods; Respiratory System SC Biochemistry & Molecular Biology; Respiratory System GA DL6LC UT WOS:000375749700001 PM 26954510 ER PT J AU Pleil, JD Isaacs, KK AF Pleil, Joachim D. Isaacs, Kristin K. TI High-resolution mass spectrometry: basic principles for using exact mass and mass defect for discovery analysis of organic molecules in blood, breath, urine and environmental media SO JOURNAL OF BREATH RESEARCH LA English DT Editorial Material DE DSSTox database; discovery analysis; toxicity testing ID RISK-ASSESSMENT; BIOMARKER DATA; IN-VITRO; CHROMATOGRAPHY; EXPOSURE; BIOINFORMATICS; IDENTIFICATION; TOXICOLOGY; EVOLUTION; WATER C1 [Pleil, Joachim D.; Isaacs, Kristin K.] US EPA, Exposure Methods & Measurements Div, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. RP Pleil, JD (reprint author), US EPA, Exposure Methods & Measurements Div, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. EM pleil.joachim@epa.gov NR 46 TC 5 Z9 5 U1 7 U2 12 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1752-7155 EI 1752-7163 J9 J BREATH RES JI J. Breath Res. PD MAR PY 2016 VL 10 IS 1 AR 012001 DI 10.1088/1752-7155/10/1/012001 PG 10 WC Biochemical Research Methods; Respiratory System SC Biochemistry & Molecular Biology; Respiratory System GA DL6LC UT WOS:000375749700002 PM 26954610 ER PT J AU Beever, EA O'Leary, J Mengelt, C West, JM Julius, S Green, N Magness, D Petes, L Stein, B Nicotra, AB Hellmann, JJ Robertson, AL Staudinger, MD Rosenberg, AA Babij, E Brennan, J Schuurman, GW Hofmann, GE AF Beever, Erik A. O'Leary, John Mengelt, Claudia West, Jordan M. Julius, Susan Green, Nancy Magness, Dawn Petes, Laura Stein, Bruce Nicotra, Adrienne B. Hellmann, Jessica J. Robertson, Amanda L. Staudinger, Michelle D. Rosenberg, Andrew A. Babij, Eleanora Brennan, Jean Schuurman, Gregor W. Hofmann, Gretchen E. TI Improving Conservation Outcomes with a New Paradigm for Understanding Species' Fundamental and Realized Adaptive Capacity SO CONSERVATION LETTERS LA English DT Article DE Climate adaptation; climate change; conservation management; fundamental adaptive capacity; policy-relevant research questions; realized adaptive capacity; vulnerability assessment ID CLIMATE-CHANGE; PHENOTYPIC PLASTICITY; CHANGING CLIMATE; EVOLUTIONARY; ADAPTATION; RESPONSES; BIODIVERSITY; POPULATION; MODELS; FUTURE AB Worldwide, many species are responding to ongoing climate change with shifts in distribution, abundance, phenology, or behavior. Consequently, natural-resource managers face increasingly urgent conservation questions related to biodiversity loss, expansion of invasive species, and deteriorating ecosystem services. We argue that our ability to address these questions is hampered by the lack of explicit consideration of species' adaptive capacity (AC). AC is the ability of a species or population to cope with climatic changes and is characterized by three fundamental components: phenotypic plasticity, dispersal ability, and genetic diversity. However, few studies simultaneously address all elements; often, AC is confused with sensitivity or omitted altogether from climate-change vulnerability assessments. Improved understanding, consistent definition, and comprehensive evaluations of AC are needed. Using classic ecological-niche theory as an analogy, we propose a new paradigm that considers fundamental and realized AC: the former reflects aspects inherent to species, whereas the latter denotes how extrinsic factors constrain AC to what is actually expressed or observed. Through this conceptualization, we identify ecological attributes contributing to AC, outline areas of research necessary to advance understanding of AC, and provide examples demonstrating how the inclusion of AC can better inform conservation and natural-resource management. C1 [Beever, Erik A.] US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. [Beever, Erik A.] Montana State Univ, Dept Ecol, Bozeman, MT 59715 USA. [O'Leary, John] Massachusetts Div Fisheries & Wildlife, 100 Hartwell St, West Boylston, MA 01583 USA. [Mengelt, Claudia] CNR, 500 Fifth St NW, Washington, DC 20001 USA. [West, Jordan M.; Julius, Susan] US EPA, Off Res & Dev, 1200 Penn Ave 8601P, Washington, DC 20460 USA. [Green, Nancy] US Fish & Wildlife Serv, Ecol Serv Program, Washington, DC 20240 USA. [Magness, Dawn] US Fish & Wildlife Serv, Kenai Natl Wildlife Refuge, Soldotna, AK 99669 USA. [Petes, Laura] NOAA, Climate Program Off, Silver Spring, MD 20910 USA. [Stein, Bruce] Natl Wildlife Federat, Washington, DC 20006 USA. [Nicotra, Adrienne B.] Australian Natl Univ, Res Sch Biol, GPO Box 4, Canberra, ACT 0200, Australia. [Hellmann, Jessica J.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. [Robertson, Amanda L.] US Fish & Wildlife Serv, Sci Applicat, Fairbanks, AK 99701 USA. [Robertson, Amanda L.] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Staudinger, Michelle D.] Northeast Climate Sci Ctr, Dept Interior, Amherst, MA 01003 USA. [Staudinger, Michelle D.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Rosenberg, Andrew A.] Union Concerned Scientists, Ctr Sci & Democracy, Cambridge, MA 02138 USA. [Babij, Eleanora] US Fish & Wildlife Serv, Migratory Bird Program, Washington, DC 20240 USA. [Brennan, Jean] US Fish & Wildlife Serv, Appalachian Landscape Conservat Cooperat, Shepherdstown, WV 25443 USA. [Schuurman, Gregor W.] Natl Pk Serv, Nat Resource Stewardship & Sci, Ft Collins, CO 80525 USA. [Schuurman, Gregor W.] Wisconsin Dept Nat Resources, Madison, WI 53707 USA. [Hofmann, Gretchen E.] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA. RP Beever, EA (reprint author), US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. EM EBeever@usgs.gov RI Nicotra, Adrienne/C-1361-2009; OI Staudinger, Michelle/0000-0002-4535-2005 FU Great Basin LCC; U.S. Geological Survey; National Wildlife Federation FX We are grateful for critical comments on earlier drafts by F.S. Chapin III, H. Coleman, M.W. Schwartz, and N.L. Stephenson. Research in Box 1 on pikas was supported by the Great Basin LCC, U.S. Geological Survey, and National Wildlife Federation. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of any of the authors' institutions or agencies. NR 35 TC 10 Z9 10 U1 7 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-263X J9 CONSERV LETT JI Conserv. Lett. PD MAR-APR PY 2016 VL 9 IS 2 BP 131 EP 137 DI 10.1111/conl.12190 PG 7 WC Biodiversity Conservation SC Biodiversity & Conservation GA DK2XV UT WOS:000374778600007 ER PT J AU Davis, AJS Singh, KK Thill, JC Meentemeyer, RK AF Davis, Amy J. S. Singh, Kunwar K. Thill, Jean-Claude Meentemeyer, Ross K. TI Accounting for residential propagule pressure improves prediction of urban plant invasion SO ECOSPHERE LA English DT Article DE Chinese privet; force of invasion; generalist invader; habitat suitability model; human-mediated invasion pressure; invasive shrub; Ligustrum sinense; plant invasion; propagule pressure; species distribution model; urban forest ID SPECIES DISTRIBUTION MODELS; WHITE-TAILED DEER; SPATIAL AUTOCORRELATION; BIOLOGICAL INVASIONS; CHINESE PRIVET; EXOTIC PLANT; DISPERSAL; HABITAT; ECOLOGY; FOREST AB Plant invasions substantially impact the ecosystem services provided by forests in urbanizing regions. Knowing where invasion risk is greatest helps target early detection and eradication efforts, but developing an accurate predictive model of invasive species presence and spread on the basis of habitat suitability remains a challenge due to spatial variation in propagule pressure (the number of individuals released) which is likely conflated with suitability. In addition to neighborhood propagule pressure that originates with propagules dispersing from naturalized populations within invaded habitats, we expect residential propagule pressure arising from the widespread use of exotic plants in the yards of single-family residences to be an important driver of invasions, and to notably improve the predictive accuracy of species distribution models (SDMs). To this end, we collected presence/absence data for a widespread forest invader, Ligustrum sinense (Chinese privet), from 400 stratified random plots located along an urban gradient across the Charlotte, North Carolina metropolitan area. We assessed the relative contribution of residential propagule pressure and neighborhood propagule pressure to improving the predictive performance of a probit SDM for Chinese privet that only contains environmental predictors. Our results indicate that, although the environment-only model predicted the highest geographic area to be at risk of invasion by privet, it also had the highest rate of failure to accurately predict observed privet occurrences as indicated by the omission (incorrectly predicted absence) and commission (incorrectly predicted presence) error rates. Accounting for residential propagule pressure substantially improved model performance by reducing the omission error by nearly 50%, thereby improving upon the ability of the model to predict privet invasion in suboptimal habitat. Given that this increase in detection was accompanied by a decrease in the geographic area predicted at risk, we conclude that SDMs for invasive exotic shrubs and potentially for other synanthropic generalist plants may be highly inefficient when residential propagule pressure is not accounted for. Accounting for residential propagule pressure in models of invasive plants results in a more focused and accurate prediction of the area at risk, thus enabling decision makers to feasibly prioritize regional scale monitoring and control efforts. C1 [Davis, Amy J. S.; Thill, Jean-Claude] Univ N Carolina, Dept Geog & Earth Sci, 9201 Univ City Blvd, Charlotte, NC 28223 USA. [Singh, Kunwar K.; Meentemeyer, Ross K.] N Carolina State Univ, Ctr Geospatial Analyt, 5180 Jordan Hall, Raleigh, NC 27695 USA. [Singh, Kunwar K.; Meentemeyer, Ross K.] N Carolina State Univ, Dept Forestry & Environm Resources, 3122 Jordan Hall, Raleigh, NC 27695 USA. [Davis, Amy J. S.] US EPA, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Singh, Kunwar K.] Univ Saskatchewan, Dept Biol, Northern Plant Ecol Lab, Saskatoon, SK S7N 5E2, Canada. RP Davis, AJS (reprint author), Univ N Carolina, Dept Geog & Earth Sci, 9201 Univ City Blvd, Charlotte, NC 28223 USA.; Davis, AJS (reprint author), US EPA, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM Davis.Amy@epa.gov FU UNC Charlotte Graduate School in the form of a fellowship FX Funding for this research was provided by the UNC Charlotte Graduate School in the form of a fellowship to A. Davis. J. Murrey Atkins Library at UNC Charlotte provided partial funding for the publication fees associated with this study. We thank Christa Rogers from the Mecklenburg Department of Natural Resources for providing us with their invasive survey data. We also thank two anonymous reviewers, whose comments greatly enhanced the manuscript. NR 66 TC 0 Z9 0 U1 8 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAR PY 2016 VL 7 IS 3 AR e01232 DI 10.1002/ecs2.1232 PG 14 WC Ecology SC Environmental Sciences & Ecology GA DK4OB UT WOS:000374896800010 ER PT J AU Hollister, JW Milstead, WB Kreakie, BJ AF Hollister, Jeffrey W. Milstead, W. Bryan Kreakie, Betty J. TI Modeling lake trophic state: a random forest approach SO ECOSPHERE LA English DT Article DE cyanobacteria; harmful algal blooms; National Lakes Assessment; nutrients; open science ID CONDITIONAL-PROBABILITY ANALYSIS; LAND-COVER DATABASE; WATER-QUALITY; UNITED-STATES; CLIMATE-CHANGE; FRESH-WATER; EUTROPHICATION; CLASSIFICATION; SCALE; ECOSYSTEMS AB Productivity of lentic ecosystems is well studied, and it is widely accepted that as nutrient inputs increase, productivity increases and lakes transition from lower trophic state (e.g., oligotrophic) to higher trophic states (e.g., eutrophic). These broad trophic state classifications are good predictors of ecosystem condition, services (e.g., recreation and esthetics), and disservices (e.g., harmful algal blooms). While the relationship between nutrients and trophic state provides reliable predictions, it requires in situ water quality data to parameterize the model. This limits the application of these models to lakes with existing and, more importantly, available water quality data. To address this, we take advantage of the availability of a large national lakes water quality database (i.e., the National Lakes Assessment), land-use/land-cover data, lake morphometry data, and other universally available data, and we apply data-mining approaches to predict trophic state. Using these data and random forests, we first model chlorophyll a and then classify the resultant predictions into trophic states. The full model estimates chlorophyll a with both in situ and universally available data. The mean-squared error and adjusted R-2 of this model was 0.09 and 0.8, respectively. The second model uses universally available GIS data only. The mean-squared error was 0.22, and the adjusted R-2 was 0.48. The Kappa coefficients of the trophic state classifications derived from the chlorophyll a predictions were 0.57 for the full model and 0.29 for the GIS-only model. Random forests extend the usefulness of the class predictions by providing prediction probabilities for each lake. This allows us to make trophic state predictions and also indicate the level of uncertainty around those predictions. For the full model, these predicted class probabilities ranged from 0.42 to 1. For the GIS-only model, they ranged from 0.33 to 0.96. It is our conclusion that in situ data are required for better predictions, yet GIS and universally available data provide trophic state predictions, with estimated uncertainty, that still have the potential for a broad array of applications. The source code and data for this manuscript are available from . C1 [Hollister, Jeffrey W.; Milstead, W. Bryan; Kreakie, Betty J.] US EPA, Atlantic Ecol Div, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, 27 Tarzwell Dr, Narragansett, RI 02882 USA. RP Hollister, JW (reprint author), US EPA, Atlantic Ecol Div, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, 27 Tarzwell Dr, Narragansett, RI 02882 USA. EM hollister.jeff@epa.gov OI Hollister, Jeffrey/0000-0002-9254-9740 NR 49 TC 0 Z9 0 U1 10 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAR PY 2016 VL 7 IS 3 AR e01321 DI 10.1002/ecs2.1321 PG 14 WC Ecology SC Environmental Sciences & Ecology GA DK4OB UT WOS:000374896800042 ER PT J AU Long, SC Zhu, Y Jang, C Lin, CJ Wang, SX Zhao, B Gao, J Deng, S Xie, JP Qiu, XZ AF Long, Shicheng Zhu, Yun Jang, Carey Lin, Che-Jen Wang, Shuxiao Zhao, Bin Gao, Jian Deng, Shuang Xie, Junping Qiu, Xuezhen TI A case study of development and application of a streamlined control and response modeling system for PM2.5 attainment assessment in China SO JOURNAL OF ENVIRONMENTAL SCIENCES LA English DT Review DE Response surface model; RSM-Linear coupled fitting; Air quality modeling; Attainment assessment; PM2.5 ID YANGTZE-RIVER DELTA; CHEMICAL-TRANSPORT MODEL; QUALITY CMAQ MODEL; AIR-QUALITY; CONTROL POLICIES; EMISSION CHANGES; POWER-PLANTS; POLLUTION; IMPACTS; REGION AB This article describes the development and application of a streamlined air control and response modeling system with a novel response surface modeling-linear coupled fitting method and a new module to provide streamlined model data for PM2.5 attainment assessment in China. This method is capable of significantly reducing the dimensions required to establish a response surface model, as well as capturing more realistic response of PM2.5 to emission changes with a limited number of model simulations. The newly developed module establishes a data link between the system and the Software for Model Attainment Test-Community Edition (SMAT-CE), and has the ability to rapidly provide model responses to emission control scenarios for SMAT-CE using a simple interface. The performance of this streamlined system is demonstrated through a case study of the Yangtze River Delta (YRD) in China. Our results show that this system is capable of reproducing the Community Multi-Scale Air Quality (CMAQ) model simulation results with maximum mean normalized error <3.5%. It is also demonstrated that primary emissions make a major contribution to ambient levels of PM2.5 in January and August (e.g., more than 50% contributed by primary emissions in Shanghai), and Shanghai needs to have regional emission control both locally and in its neighboring provinces to meet China's annual PM2.5 National Ambient Air Quality Standard. The streamlined system provides a real-time control/response assessment to identify the contributions of major emission sources to ambient PM2.5 (and potentially O-3 as well) and streamline air quality data for SMAT-CE to perform attainment assessments. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. C1 [Long, Shicheng; Zhu, Yun; Lin, Che-Jen; Xie, Junping; Qiu, Xuezhen] S China Univ Technol, Guangzhou Higher Educ Mega Ctr, Sch Environm & Energy, Guangzhou 510006, Guangdong, Peoples R China. [Jang, Carey] US EPA, Off Air Qual Planning & Stand, Res Triangle Pk, NC 27711 USA. [Lin, Che-Jen] Lamar Univ, Dept Civil Engn, Beaumont, TX 77710 USA. [Wang, Shuxiao; Zhao, Bin] Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China. [Gao, Jian; Deng, Shuang] Chinese Res Inst Environm Sci, Beijing 100012, Peoples R China. RP Zhu, Y (reprint author), S China Univ Technol, Guangzhou Higher Educ Mega Ctr, Sch Environm & Energy, Guangzhou 510006, Guangdong, Peoples R China. EM zhuyun@scut.edu.cn RI wang, shuxiao/H-5990-2011; Lin, Che-Jen/K-1808-2013 OI wang, shuxiao/0000-0001-9727-1963; Lin, Che-Jen/0000-0001-5990-3093 FU US Environmental Protection Agency [OR13810-001.04 A10-0223-S001-A02]; Guangzhou Environmental Protection Bureau [x2hjB2150020]; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control [2011A060901011]; Chinese Academy of Sciences [XDB05030400]; Ministry of Environmental Protection's Special Funds for Research on Public Welfare [201409002]; Guangdong Provincial Department of Science and Technology [2014A050503019] FX Financial support and data source for this work is provided by the US Environmental Protection Agency (No. OR13810-001.04 A10-0223-S001-A02) and Guangzhou Environmental Protection Bureau (No. x2hjB2150020), the project of an integrated modeling and filed observational verification on the deposition of typical industrial point-source mercury emissions in the Pearl River Delta. This work is also partly supported by the funding of Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control (No. 2011A060901011), the project of Atmospheric Haze Collaboration Control Technology Design (No. XDB05030400) from the Chinese Academy of Sciences and the Ministry of Environmental Protection's Special Funds for Research on Public Welfare (No. 201409002). Partly financial support is also provided by the Guangdong Provincial Department of Science and Technology, the project of demonstration research of air quality management cost-benefit analysis and attainment assessments technology (No. 2014A050503019). NR 36 TC 2 Z9 2 U1 10 U2 20 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1001-0742 EI 1878-7320 J9 J ENVIRON SCI-CHINA JI J. Environ. Sci. PD MAR 1 PY 2016 VL 41 BP 69 EP 80 DI 10.1016/j.jes.2015.05.019 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA DI1BQ UT WOS:000373231400009 PM 26969052 ER PT J AU Konar, M Reimer, JJ Hussein, Z Hanasaki, N AF Konar, Megan Reimer, Jeffrey J. Hussein, Zekarias Hanasaki, Naota TI The water footprint of staple crop trade under climate and policy scenarios SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE water footprint; agricultural trade; virtual water; global water savings; climate change; trade policy; adaptation ID AGRICULTURAL TRADE; INTERNATIONAL-TRADE; INTEGRATED MODEL; FOOD; SUSTAINABILITY; SENSITIVITY; HUMANITY; SAVINGS; IMPACT AB Trade in staple crop commodities has become increasingly important in the global food system, with ramifications for both food security and water resources sustainability. It is thus essential to understand how the water footprint (WF) of staple crop trade may change in the future. To this end, we project international staple crop trade and its WF under climate and policy scenarios for the year 2030. We use the H08 global hydrologic model to determine the impact of climatic changes to staple crop yields and evapotranspiration. Using the yield changes projected with the H08 model, we estimate the bilateral trade of staple crops using the Global Trade Analysis Project model. We combine these projections to obtain the total and blue WF of agricultural trade and global water savings (GWS) across scenarios. This approach enables us to determine the direct impact of climate change and trade liberalization-together and in isolation-on the WF of staple crop trade. Importantly, we show that trade liberalization leads to greater WF, making it a potentially important adaptation measure to a changing climate, although future work is needed to distinguish high resolution crop water use, water stress, and commodity transfers. C1 [Konar, Megan] Univ Illinois, Civil & Environm Engn Dept, Urbana, IL 61801 USA. [Reimer, Jeffrey J.] Oregon State Univ, Dept Appl Econ, Corvallis, OR 97331 USA. [Hussein, Zekarias] US EPA, Washington, DC 20460 USA. [Hanasaki, Naota] Natl Inst Environm Studies, Tsukuba, Ibaraki 3058506, Japan. RP Konar, M (reprint author), Univ Illinois, Civil & Environm Engn Dept, Urbana, IL 61801 USA. EM mkonar@illinois.edu RI Hanasaki, Naota/C-2932-2009 OI Hanasaki, Naota/0000-0002-5092-7563 NR 62 TC 1 Z9 1 U1 24 U2 44 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2016 VL 11 IS 3 AR 035006 DI 10.1088/1748-9326/11/3/035006 PG 15 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DI3LY UT WOS:000373401400034 ER PT J AU Tian, XH Sohngen, B Kim, JB Ohrel, S Cole, J AF Tian, Xiaohui Sohngen, Brent Kim, John B. Ohrel, Sara Cole, Jefferson TI Global climate change impacts on forests and markets SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE climate change; dynamic optimization; markets ID TIMBER MARKETS; SECTOR; ECOSYSTEMS; PRODUCTS; DEMAND; MODEL AB This paper develops an economic analysis of climate change impacts in the global forest sector. It illustrates how potential future climate change impacts can be integrated into a dynamic forestry economics model using data from a global dynamic vegetation model, the MC2 model. The results suggest that climate change will cause forest outputs (such as timber) to increase by approximately 30% over the century. Aboveground forest carbon storage also is projected to increase, by approximately 26 Pg C by 2115, as a result of climate change, potentially providing an offset to emissions from other sectors. The effects of climate mitigation policies in the energy sector are then examined. When climate mitigation in the energy sector reduces warming, we project a smaller increase in forest outputs over the timeframe of the analysis, and we project a reduction in the sink capacity of forests of around 12 Pg C by 2115. C1 [Tian, Xiaohui] Renmin Univ, 59 Zhongguancun Ave,Room 919 Mingde Main Bldg, Beijing 100872, Peoples R China. [Sohngen, Brent] Ohio State Univ, 2120 Fyffe Rd, Columbus, OH 43210 USA. [Kim, John B.] US Forest Serv, Pacific NW Res Stn, USDA, 3200 SW Jefferson Way, Corvallis, OR 97330 USA. [Ohrel, Sara; Cole, Jefferson] US EPA, Climate Change Div, 1200 Penn Ave,NW 6207-J, Washington, DC 20460 USA. RP Tian, XH (reprint author), Renmin Univ, 59 Zhongguancun Ave,Room 919 Mingde Main Bldg, Beijing 100872, Peoples R China.; Sohngen, B (reprint author), Ohio State Univ, 2120 Fyffe Rd, Columbus, OH 43210 USA. EM tianxiaohui@ruc.edu.cn; Sohngen.1@osu.edu FU US Forest Service Western Wildland Environmental Threat Assessment Center; US Environmental Protection Agency's Climate Change Division [DW-012-92388301]; US Environmental Protection Agency Climate Change Division FX The authors appreciate funding from the US Environmental Protection Agency Climate Change Division although the analysis and conclusion do not in any way represent the views of the US Environmental Protection Agency Climate Change Division. John B Kim is supported in part by the US Forest Service Western Wildland Environmental Threat Assessment Center, and by US Environmental Protection Agency's Climate Change Division, under interagency agreement DW-012-92388301. NR 21 TC 0 Z9 0 U1 3 U2 11 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2016 VL 11 IS 3 AR 035011 DI 10.1088/1748-9326/11/3/035011 PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DI3LY UT WOS:000373401400039 ER PT J AU Boyes, WK Bercegeay, M Degn, L Beasley, TE Evansky, PA Mwanza, JC Geller, AM Pinckney, C Nork, TM Bushnell, PJ AF Boyes, William K. Bercegeay, Mark Degn, Laura Beasley, Tracey E. Evansky, Paul A. Mwanza, Jean Claude Geller, Andrew M. Pinckney, Charles Nork, T. Michael Bushnell, Philip J. TI Toluene inhalation exposure for 13 weeks causes persistent changes in electroretinograms of Long-Evans rats SO NEUROTOXICOLOGY LA English DT Article DE Toluene; Retina; Electroretinograms; Visual function ID COLOR-VISION LOSS; SENSORY-EVOKED-POTENTIALS; ORGANIC-SOLVENT MIXTURES; CENTRAL-NERVOUS-SYSTEM; NAKA-RUSHTON EQUATION; CONTRAST-SENSITIVITY; OCCUPATIONAL-EXPOSURE; PERCHLOROETHYLENE EXPOSURE; VISUAL DYSFUNCTION; B-WAVE AB Studies of humans chronically exposed to volatile organic solvents have reported impaired visual functions, including low contrast sensitivity and reduced color discrimination. These reports, however, lacked confirmation from controlled laboratory experiments. To address this question experimentally, we examined visual function by recording visual evoked potentials (VEP) and/or electroretinograms (ERG) from four sets of rats exposed repeatedly to toluene. In addition, eyes of the rats were examined with an ophthalmoscope and some of the retinal tissues were evaluated for rod and M-cone photoreceptor immunohistochemistry. The first study examined rats following exposure to 0, 10, 100 or 1000 ppm toluene by inhalation (6 hr/d, 5 d/wk) for 13 weeks. One week after the termination of exposure, the rats were implanted with chronically indwelling electrodes and the following week pattern-elicited VEPs were recorded. VEP amplitudes were not significantly changed by toluene exposure. Four to five weeks after completion of exposure, rats were dark-adapted overnight, anesthetized, and several sets of electroretinograms (ERG) were recorded. In dark-adapted ERGs recorded over a 5-log (cd-s/m(2)) range of flash luminance, b-wave amplitudes were significantly reduced at high stimulus luminance values in rats previously exposed to 1000 ppm toluene. A second set of rats, exposed concurrently with the first set, was tested approximately one year after the termination of 13 weeks of exposure to toluene. Again, dark-adapted ERG b-wave amplitudes were reduced at high stimulus luminance values in rats previously exposed to 1000 ppm toluene. A third set of rats was exposed to the same concentrations of toluene for only 4 weeks, and a fourth set of rats exposed to 0 or 1000 ppm toluene for 4 weeks were tested approximately 1 year after the completion of exposure. No statistically significant reductions of ERG b-wave amplitude were observed in either set of rats exposed for 4 weeks. No significant changes were observed in ERG a-wave amplitude or latency, b-wave latency, UV- or green-flicker ERGs, or in photopic flash ERGs. There were no changes in the density of rod or M-cone photoreceptors. The ERG b-wave reflects the firing patterns of on-bipolar cells. The reductions of b-wave amplitude after 13 weeks of exposure and persisting for 1 year suggest that alterations may have occurred in the inner nuclear layer of the retina, where the bipolar cells reside, or the outer or inner plexiform layers where the bipolar cells make synaptic connections. These data provide experimental evidence that repeated exposure to toluene may lead to subtle persistent changes in visual function. The fact that toluene affected ERGs, but not VEPs, suggests that elements in the rat retina may be more sensitive to organic solvent exposure than the rat visual cortex. Published by Elsevier Inc. C1 [Boyes, William K.; Bercegeay, Mark; Degn, Laura; Beasley, Tracey E.; Bushnell, Philip J.] US EPA, Tox Assessment Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Evansky, Paul A.] US EPA, Environm Publ Hlth Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Mwanza, Jean Claude] Univ N Carolina, Dept Ophthalmol, Chapel Hill, NC USA. [Geller, Andrew M.] US EPA, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Pinckney, Charles] Livingstone Coll, Salisbury, NC USA. [Nork, T. Michael] Univ Wisconsin, Sch Med & Publ Hlth, Dept Ophthalmol & Visual Sci, 600 Highland Ave,K6-456, Madison, WI 53792 USA. RP Boyes, WK (reprint author), US EPA, Tox Assessment Div, B105-04, Res Triangle Pk, NC 27711 USA. EM boyes.william@epa.gov FU EPA contract [EP07D000314]; National Institutes of Health (NIH) [P30 EY016665]; Research to Prevent Blindness; National Research Council, Washington, DC FX The authors thank Sheppard Martin, Timothy J. Shafer, Donald A. Fox, and Prasada Kodavanti for comments on earlier versions of the manuscript. Retinal immunochemistry (TMN) was supported by EPA contract # EP07D000314, by National Institutes of Health (NIH) grant P30 EY016665 (University of Wisconsin School of Medicine and Public Health) and by Research to Prevent Blindness. Jean Claude Mwanza was supported by the National Research Council, Washington, DC. M. Ian Gilmour coordinated the inhalation studies. We thank Charles Hamm, Paul Killough, Earl Puckett, and E. Baker Bailey for engineering support. In addition, Doc Terrell, William Anderson, Justin Bradshaw, Jessica Collins, Cary Copeland, Jamie DeWitt, Wendy Oshiro, and Connie Meacham assisted with the toluene exposures. Allen J. Irgens performed the retinal immunohistochemistry. This manuscript is fondly dedicated to the memory of E. Baker Bailey. NR 103 TC 0 Z9 0 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0161-813X EI 1872-9711 J9 NEUROTOXICOLOGY JI Neurotoxicology PD MAR PY 2016 VL 53 BP 257 EP 270 DI 10.1016/j.neuro.2016.02.008 PG 14 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA DI3UK UT WOS:000373423800029 PM 26899397 ER PT J AU Herr, DW Freeborn, DL Degn, L Martin, SA Ortenzio, J Pantlin, L Hamm, CW Boyes, WK AF Herr, David W. Freeborn, Danielle L. Degn, Laura Martin, Sheppard A. Ortenzio, Jayna Pantlin, Lara Hamm, Charles W. Boyes, William K. TI Neurophysiological assessment of auditory, peripheral nerve, somatosensory, and visual system function after developmental exposure to gasoline, E15, and E85 vapors SO NEUROTOXICOLOGY AND TERATOLOGY LA English DT Article DE Gasoline vapor; E15 vapor, E85 vapor, developmental neurotoxicity; Nerve conduction velocity; Sensory evoked potentials; Electroretinogram ID SENSORY EVOKED-POTENTIALS; HEALTH-ASSESSMENT; PHARMACOKINETIC MODELS; REPRODUCTIVE TOXICITY; CONTRAST SENSITIVITY; COGNITIVE DEFICITS; PRENATAL EXPOSURE; ADULT RATS; ETHANOL; MIXTURES AB The use of gasolines blended with a range of ethanol concentrations may result in inhalation of vapors containing a variable combination of ethanol with other volatile gasoline constituents. The possibility of exposure and potential interactions between vapor constituents suggests the need to evaluate the possible risks of this complex mixture. Previously we evaluated the effects of developmental exposure to ethanol vapors on neurophysiological measures of sensory function as a component of a larger project evaluating developmental ethanol toxicity. Here we report an evaluation using the same battery of sensory function testing in offspring of pregnant dams exposed during gestation to condensed vapors of gasoline (E0), gasoline blended with 15% ethanol (E15) or gasoline blended with 85% ethanol (E85). Pregnant Long-Evans rats were exposed to target concentrations 0, 3000, 6000, or 9000 ppm total hydrocarbon vapors for 6.5 h/day over GD9 - GD20. Sensory evaluations of male offspring began as adults. The electrophysiological testing battery included tests of: peripheral nerve (compound action potentials, nerve conduction velocity [NCV]), somatosensory (cortical and cerebellar evoked potentials), auditory (brainstem auditory evoked responses), and visual functions. Visual function assessment included pattern elicited visual evoked potentials (VEP), VEP contrast sensitivity, dark-adapted (scotopic) electroretinograms (ERGs), light-adapted (photopic) ERGs, and green flicker ERGs. The results included sporadic statistically significant effects, but the observations were not consistently concentration-related and appeared to be statistical Type 1 errors related to multiple dependent measures evaluated. The exposure concentrations were much higher than can be reasonably expected from typical exposures to the general population during refueling or other common exposure situations. Overall the results indicate that gestational exposure of male rats to ethanol/gasoline vapor combinations did not cause detectable changes in peripheral nerve, somatosensory, auditory, or visual function when the offspring were assessed as adults. Published by Elsevier Inc. C1 [Herr, David W.; Freeborn, Danielle L.; Degn, Laura; Martin, Sheppard A.; Hamm, Charles W.; Boyes, William K.] US EPA, Neurotoxicol Branch, Tox Assessment Div, NHEERL,ORD, MD B105-04, Res Triangle Pk, NC 27711 USA. [Ortenzio, Jayna] US EPA, Res Triangle Pk, NC 27711 USA. [Pantlin, Lara] Meredith Coll, Raleigh, NC 27607 USA. RP Herr, DW (reprint author), US EPA, NHEERL TAD NB, 109 TW Alexander Dr,MD B105-04, Res Triangle Pk, NC 27711 USA. EM Herr.david@epa.gov; Freeborn.danielle@epa.gov; Degn.laura@epa.gov; sam506@gmail.com; Ortenzio.jayna@epa.gov; lpantlin@rams.colostate.edu; Hamm.charlesw@epa.gov; Boyes.william@epa.gov FU EPA [EP-D-11-061] FX The authors would like to thank Drs. Timothy Shafer and John Cowden for their insightful review of an earlier version of this manuscript. We also acknowledge Chevron for preparation of vapor condensates under EPA Contract EP-D-11-061. We express thanks to Mrs. Tracey Beasley for technical support during surgical procedures, Paul Evansky for operation of the exposure chambers, Harmon Harrell, Brenda Proctor, and Mike McFarland for their maintenance and care of rats that were evaluated in these studies. We would also like to thank John Havel for his assistance with the graphical abstract. NR 39 TC 0 Z9 0 U1 1 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0892-0362 EI 1872-9738 J9 NEUROTOXICOL TERATOL JI Neurotoxicol. Teratol. PD MAR-APR PY 2016 VL 54 BP 78 EP 88 DI 10.1016/j.ntt.2015.12.006 PG 11 WC Neurosciences; Toxicology SC Neurosciences & Neurology; Toxicology GA DI5QE UT WOS:000373552900011 PM 26721698 ER PT J AU Sethi, PK White, CA Cummings, BS Hines, RN Muralidhara, S Bruckner, JV AF Sethi, Pankaj K. White, Catherine A. Cummings, Brian S. Hines, Ronald N. Muralidhara, Srinivasa Bruckner, James V. TI Ontogeny of plasma proteins, albumin and binding of diazepam, cyclosporine, and deltamethrin SO PEDIATRIC RESEARCH LA English DT Article ID PHYSIOLOGICALLY-BASED PHARMACOKINETICS; ALPHA-1-ACID GLYCOPROTEIN; DEVELOPMENTAL EXPRESSION; DRUG DISCOVERY; RISK-ASSESSMENT; AGE; SERUM; INFANTS; LIPOPROTEINS; CHILDREN AB BACKGROUND: To characterize the ontogeny of plasma albumin and total proteins, due to the lack of a comprehensive pediatric database. Secondly, to establish the magnitude and duration of maturational changes in binding of highly-bound drugs/chemicals. METHODS: Anonymized plasma samples from 296 donors were pooled in 6 age brackets from birth to adolescence. Total protein and albumin levels were measured in each age group, as was the age-dependency of plasma binding of diazepam (DZP), cyclosporine (CYC), and deltamethrin (DLM), a pyrethroid insecticide. RESULTS: Plasma levels of albumin and total proteins steadily increased for the first 1-3 y of life. Unbound DZP and CYC fractions were elevated three- to fourfold in neonates, but decreased to adult levels after 1 and 3 y, respectively. Unbound DLM levels exceeded those in adults for just 1 mo. CONCLUSION: Neonates and infants under 1-3 y may be at risk from increased amounts of free drug, when given standard doses of some highly-bound drugs. Pyrethroid insecticides might be anticipated to pose increased risk for 1 mo. C1 [Sethi, Pankaj K.; White, Catherine A.; Cummings, Brian S.; Muralidhara, Srinivasa; Bruckner, James V.] Univ Georgia, Coll Pharm, Dept Pharmaceut & Biomed Sci, Athens, GA 30602 USA. [Sethi, Pankaj K.] US EPA, Natl Exposure Res Lab, Athens, GA USA. [Hines, Ronald N.] Med Coll Wisconsin, Dept Pediat, 8701 Watertown Plank Rd, Milwaukee, WI 53226 USA. [Hines, Ronald N.] Childrens Hosp & Hlth Syst, Childrens Res Inst, Milwaukee, WI USA. [Hines, Ronald N.] Med Coll Wisconsin, Dept Pharmacol & Toxicol, Milwaukee, WI 53226 USA. [Hines, Ronald N.] US EPA, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Bruckner, JV (reprint author), Univ Georgia, Coll Pharm, Dept Pharmaceut & Biomed Sci, Athens, GA 30602 USA. EM bruckner@uga.edu OI Hines, Ronald/0000-0002-3094-4200 FU Council for Advancement of Pyrethroid Human Risk Assessment (CAPHRA), NW, Suite, Washington, DC FX This study was supported in part by the Council for Advancement of Pyrethroid Human Risk Assessment (CAPHRA), 1667K Street NW, Suite 300, Washington, DC 20006. CAPHRA did not play a role in: the study design; collection, analysis, and interpretation of data; writing of the report; or the decision to submit the manuscript for publication. No honorarium, grant, or other form of payment was received to produce the manuscript. NR 45 TC 4 Z9 4 U1 3 U2 8 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0031-3998 EI 1530-0447 J9 PEDIATR RES JI Pediatr. Res. PD MAR PY 2016 VL 79 IS 3 BP 409 EP 415 DI 10.1038/pr.2015.237 PG 7 WC Pediatrics SC Pediatrics GA DI3AY UT WOS:000373371800007 PM 26571224 ER PT J AU Green, HC Fisher, JC McLellan, SL Sogin, ML Shanks, OC AF Green, Hyatt C. Fisher, Jenny C. McLellan, Sandra L. Sogin, Mitchell L. Shanks, Orin C. TI Identification of Specialists and Abundance-Occupancy Relationships among Intestinal Bacteria of Aves, Mammalia, and Actinopterygii SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID FECAL SOURCE IDENTIFICATION; GENETIC-MARKERS; GUT MICROBIOTA; BETA-DIVERSITY; WATER; TAXA; POLLUTION; GULL; PCR; SPECIALIZATION AB The coalescence of next-generation DNA sequencing methods, ecological perspectives, and bioinformatics analysis tools is rapidly advancing our understanding of the evolution and function of vertebrate-associated bacterial communities. Delineation of host-microbe associations has applied benefits ranging from clinical treatments to protecting our natural waters. Microbial communities follow some broad-scale patterns observed for macroorganisms, but it remains unclear how the specialization of intestinal vertebrate-associated communities to a particular host environment influences broad-scale patterns in microbial abundance and distribution. We analyzed the V6 region of 16S rRNA genes amplified from 106 fecal samples spanning Aves, Mammalia, and Actinopterygii (ray-finned fish). We investigated the interspecific abundance-occupancy relationship, where widespread taxa tend to be more abundant than narrowly distributed taxa, among operational taxonomic units (OTUs) within and among host species. In a separate analysis, we identified specialist OTUs that were highly abundant in a single host and rare in all other hosts by using a multinomial model without excluding undersampled OTUs a priori. We show that intestinal microbes in humans and other vertebrates display abundance-occupancy relationships, but because intestinal host-associated communities have undergone intense specialization, this trend is violated by a disproportionately large number of specialist taxa. Although it is difficult to distinguish the effects of dispersal limitations, host selection, historical contingency, and stochastic processes on community assembly, results suggest that intestinal bacteria can be shared among diverse hosts in ways that resemble the distribution of "free-living" bacteria in the extraintestinal environment. C1 [Green, Hyatt C.] SUNY ESF, Syracuse, NY USA. [Fisher, Jenny C.; McLellan, Sandra L.] Univ Wisconsin, Milwaukee, WI 53201 USA. [Sogin, Mitchell L.] Marine Biol Lab, Josephine Bay Paul Ctr, Woods Hole, MA 02543 USA. [Shanks, Orin C.] US EPA, Cincinnati, OH 45268 USA. RP Green, HC (reprint author), SUNY ESF, Syracuse, NY USA. EM hgreen@esf.edu FU U.S. Environmental Protection Agency through its Office of Research and Development FX The U.S. Environmental Protection Agency through its Office of Research and Development funded and managed the research described here. NR 56 TC 0 Z9 0 U1 5 U2 13 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2016 VL 82 IS 5 BP 1496 EP 1503 DI 10.1128/AEM.02456-15 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DI2PL UT WOS:000373338800014 ER PT J AU Barrett, BM Neubert, PL Falatko, D Ota, A AF Barrett, B. M. Neubert, P. L. Falatko, D. Ota, A. TI A new genus and species of Eulepethidae Chamberlin, 1919 (Annelida: Polychaeta) from deep water sites off South Oahu and Hilo, Hawaii. SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 EcoAnalysts Inc, Moscow, ID USA. Stantec, Edmonton, AB, Canada. Eastern Res Grp Inc, London, England. US EPA, Reg 9, Water Div, Washington, DC USA. EM cbarrett@ecoanalysts.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA P2.120 BP E258 EP E258 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457601388 ER PT J AU Enzor, LA Hankins, C Barron, MG AF Enzor, L. A. Hankins, C. Barron, M. G. TI Ocean acidification effects on Caribbean scleractinian coral calcification using a recirculating system: A novel approach to OA research SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 [Enzor, L. A.; Hankins, C.; Barron, M. G.] US EPA, Washington, DC USA. EM enzor.laura@epa.gov NR 0 TC 0 Z9 0 U1 2 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA P3.34 BP E284 EP E284 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457601494 ER PT J AU Green, OO Garmestani, AS Albro, S Ban, NC Berland, A Burkman, CE Gardiner, MM Gunderson, L Hopton, ME Schoon, ML Shuster, WD AF Green, Olivia Odom Garmestani, Ahjond S. Albro, Sandra Ban, Natalie C. Berland, Adam Burkman, Caitlin E. Gardiner, Mary M. Gunderson, Lance Hopton, Matthew E. Schoon, Michael L. Shuster, William D. TI Adaptive governance to promote ecosystem services in urban green spaces SO URBAN ECOSYSTEMS LA English DT Article DE Urban green space; Ecosystem services; Adaptive governance; Post-industrial cities ID PROPERTY-VALUES; BIODIVERSITY CONSERVATION; BUSINESS DISTRICT; VACANT LAND; MANAGEMENT; CITY; GARDENS; IMPACT; PARKS; TREES AB Managing urban green space as part of an ongoing social-ecological transformation poses novel governance issues, particularly in post-industrial settings. Urban green spaces operate as small-scale nodes in larger networks of ecological reserves that provide and maintain key ecosystem services such as pollination, water retention and infiltration, and sustainable food production. In an urban mosaic, a myriad of social and ecological components factor into aggregating and managing land to maintain or increase the flow of ecosystem services associated with green spaces. Vacant lots (a form of urban green space) are being repurposed for multiple functions, such as habitat for biodiversity, including arthropods that provide pollination services to other green areas; to capture urban runoff that eases the burden on ageing wastewater systems and other civic infrastructure; and to reduce urban heat island effects. Because of the uncertainty and complexities of managing for ecosystem services in urban settings, we advocate for a governance approach that is adaptive and iterative in nature-adaptive governance-to address the ever changing social order underlying post-industrial cities and offer the rise of land banks as an example of governance innovation. C1 [Green, Olivia Odom] Atlantic States Legal Fdn, Syracuse, NY 13204 USA. [Garmestani, Ahjond S.; Berland, Adam; Burkman, Caitlin E.; Hopton, Matthew E.; Shuster, William D.] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Albro, Sandra] Cleveland Bot Garden, Cleveland, OH 44106 USA. [Ban, Natalie C.] Univ Victoria, Sch Environm Studies, Victoria, BC, Canada. [Gardiner, Mary M.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Gunderson, Lance] Emory Univ, Dept Environm Sci, Atlanta, GA 30322 USA. [Schoon, Michael L.] Arizona State Univ, Sch Sustainabil, Tempe, AZ 85287 USA. RP Hopton, ME (reprint author), US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. EM hopton.matthew@epa.gov OI Hopton, Matt/0000-0001-7962-6820; Berland, Adam/0000-0003-0473-6405 FU National Research Council Research Associateship Award at the United States Environmental Protection Agency FX This research was conducted with the support of an appointment to the Research Participation Program at the National Risk Management Research Laboratory administered by the Oak Ridge Institute for Science and Education (OOG, CEB). AB held a National Research Council Research Associateship Award at the United States Environmental Protection Agency. The views expressed in this paper are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. NR 90 TC 4 Z9 4 U1 40 U2 83 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 EI 1573-1642 J9 URBAN ECOSYST JI Urban Ecosyst. PD MAR PY 2016 VL 19 IS 1 BP 77 EP 93 DI 10.1007/s11252-015-0476-2 PG 17 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA DH1LR UT WOS:000372546000004 ER PT J AU Lee, CW Zhao, YX Lu, SY Stevens, WR AF Lee, Chun Wai Zhao, Yongxin Lu, Shengyong Stevens, William R. TI Catalytic Destruction of a Surrogate Organic Hazardous Air Pollutant as a Potential Co-benefit for Coal-Fired Selective Catalytic Reduction Systems SO ENERGY & FUELS LA English DT Article ID MERCURY OXIDATION; FLUE-GAS; AROMATIC-HYDROCARBONS; COMBUSTION CONDITIONS; INCINERATION PLANTS; REAL-TIME; EMISSIONS; 1,2-DICHLOROBENZENE; PARTICLES; PYROLYSIS AB Catalytic destruction of benzene (C6H6), a surrogate for organic hazardous air pollutants (HAPs) produced from coal combustion, was investigated using a commercial selective catalytic reduction (SCR) catalyst for evaluating the potential co-benefit of the SCR technology for reducing organic HAP emissions. Bench-scale experiments were performed using simulated coal combustion flue gases under a broad range of SCR reaction conditions. C6H6 was added at 1 or 17 ppm into the flue gas mixtures with different concentrations of sulfur dioxide (SO2), nitrogen oxide (NO), hydrogen chloride (HCl), and ammonia (NH3) to simulate the combustion of bituminous and sub-bituminous coals. The destruction of the C6H6 across the catalyst was measured by a total hydrocarbon analyzer and a resonance-enhanced multiphoton ionization time-of-flight mass spectrometer (REMPI-TOFMS) for the experiments with high (17 ppm) and low (1 ppm) concentrations of C6H6, respectively. The operating parameters of the SCR process, including the space velocity, temperature, and concentration of C6H6, were found to have a significant impact on the destruction of C6H6. The constituents of the flue gas had very little impact on the destruction, suggesting that the significant additional co-benefit of destruction of trace organic HAPs provided by the SCR process may be applicable to a wide variety of coals under different firing conditions. Destruction of C6H6 with high efficiencies is likely to occur concurrently with the reduction of NO during the SCR process without indication of carbon deposition on the catalyst. C1 [Lee, Chun Wai] US EPA, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Zhao, Yongxin] ARCADIS US Inc, Durham, NC 27713 USA. [Lu, Shengyong] Zhejiang Univ, Inst Thermal Power Engn, State Key Lab Clean Energy Utilizat, Hangzhou 310027, Zhejiang, Peoples R China. [Stevens, William R.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37830 USA. [Zhao, Yongxin] Albemarle Corp, Baton Rouge, LA 70805 USA. [Stevens, William R.] Kentucky Christian Univ, Coll Hlth Sci, Grayson, KY 41143 USA. RP Lee, CW (reprint author), US EPA, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. EM lee.chun-wai@epa.gov FU Ministry of Education of China through the Program of Introducing Talents of Discipline to University [B08026] FX Jeremy Lecomte (Universite d'Orleans, Orleans, France, student intern at the U.S. EPA) provided support for the experimental testing and data analysis. The authors are thankful for the support provided to one of the authors (Shengyong Lu) by the Ministry of Education of China through the Program of Introducing Talents of Discipline to University (B08026). NR 24 TC 0 Z9 0 U1 8 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD MAR PY 2016 VL 30 IS 3 BP 2240 EP 2247 DI 10.1021/acs.energyfuels.5b02058 PG 8 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA DH1RO UT WOS:000372562800080 ER PT J AU Fewell, B AF Fewell, Brent TI The Failure of Cooperative Federalism in Flint, Michigan SO JOURNAL AMERICAN WATER WORKS ASSOCIATION LA English DT Article C1 [Fewell, Brent] Earth & Water Grp, 1455 Penn Ave,Ste 400, Washington, DC 20004 USA. [Fewell, Brent] US Environm Protect Agcy, Off Water, Dallas, TX 75202 USA. RP Fewell, B (reprint author), Earth & Water Grp, 1455 Penn Ave,Ste 400, Washington, DC 20004 USA.; Fewell, B (reprint author), US Environm Protect Agcy, Off Water, Dallas, TX 75202 USA. EM brent.fewell@earthandwatergroup.com NR 1 TC 1 Z9 1 U1 8 U2 11 PU AMER WATER WORKS ASSOC PI DENVER PA 6666 W QUINCY AVE, DENVER, CO 80235 USA SN 2164-4535 J9 J AM WATER WORKS ASS JI J. Am. Water Work Assoc. PD MAR PY 2016 VL 108 IS 3 BP 12 EP 14 DI 10.5942/jawwa.2016.108.0084 PG 3 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA DG3FM UT WOS:000371954900003 ER PT J AU Jahne, MA Rogers, SW Holsen, TM Grimberg, SJ Ramler, IP Kim, S AF Jahne, Michael A. Rogers, Shane W. Holsen, Thomas M. Grimberg, Stefan J. Ramler, Ivan P. Kim, Seungo TI Bioaerosol Deposition to Food Crops near Manure Application: Quantitative Microbial Risk Assessment SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID ESCHERICHIA-COLI O157-H7; REAL-TIME PCR; LISTERIA-MONOCYTOGENES; FRESH PRODUCE; LEAFY GREENS; WATER; SURVIVAL; LETTUCE; PATHOGENS; FARM AB Production of both livestock and food crops are central priorities of agriculture; however, food safety concerns arise where these practices intersect. In this study, we investigated the public health risks associated with potential bioaerosol deposition to crops grown in the vicinity of manure application sites. A field sampling campaign at dairy manure application sites supported the emission, transport, and deposition modeling of bioaerosols emitted from these lands following application activities. Results were coupled with a quantitative microbial risk assessment model to estimate the infection risk due to consumption of leafy green vegetable crops grown at various distances downwind from the application area. Inactivation of pathogens (Salmonella spp., Campylobacter spp., and Escherichia coli O157:H7) on both the manure-amended field and on crops was considered to determine the maximum loading of pathogens to plants with time following application. Overall median one-time infection risks at the time of maximum loading decreased from 1:1300 at 0 m directly downwind from the field to 1:6700 at 100 m and 1:92,000 at 1000 m; peak risks (95th percentiles) were considerably greater (1:18, 1:89, and 1:1200, respectively). Median risk was below 1:10,000 at >160 m downwind. As such, it is recommended that a 160-m setback distance is provided between manure application and nearby leafy green crop production. Additional distance or delay before harvest will provide further protection of public health. C1 [Jahne, Michael A.; Kim, Seungo] Clarkson Univ, Inst Sustainable Environm, Potsdam, NY 13699 USA. [Rogers, Shane W.; Holsen, Thomas M.; Grimberg, Stefan J.] Clarkson Univ, Dept Civil & Environm Engn, Potsdam, NY 13699 USA. [Ramler, Ivan P.] St Lawrence Univ, Dept Math Stat & Comp Sci, Canton, NY 13617 USA. [Jahne, Michael A.] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. RP Rogers, SW (reprint author), Clarkson Univ, Dept Civil & Environm Engn, Potsdam, NY 13699 USA. EM srogers@clarkson.edu OI Rogers, Shane/0000-0003-4488-5122 FU National Research Initiative Competitive Grant [2010-65112-20556]; Agricultural Food and Research Initiative (AFRI) from the National Institute of Food and Agriculture (NIFA) Air Quality Program; Lakes Environmental Software FX This project was supported by National Research Initiative Competitive Grant no. 2010-65112-20556 and/or the Agricultural Food and Research Initiative (AFRI) from the National Institute of Food and Agriculture (NIFA) Air Quality Program. We thank Dr. William J. Mills III and Lakes Environmental Software for their support and donation of AERMOD View and the farm manager for allowing sample collection at his facilities. NR 55 TC 0 Z9 0 U1 4 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 666 EP 674 DI 10.2134/jeq2015.04.0187 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900030 PM 27065414 ER PT J AU Hoque, YM Tripathi, S Hantush, MM Govindaraju, RS AF Hoque, Yamen M. Tripathi, Shivam Hantush, Mohamed M. Govindaraju, Rao S. TI Aggregate Measures of Watershed Health from Reconstructed Water Quality Data with Uncertainty SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID PRINCIPAL COMPONENT ANALYSIS; SOCIAL-ECOLOGICAL SYSTEMS; RISK-ASSESSMENT; RIVER-BASIN; SWAT MODEL; RESILIENCE; RELIABILITY; PRECIPITATION; REDUNDANCY; DIVERSITY AB Risk-based measures such as reliability, resilience, and vulnerability (R-R-V) have the potential to serve as watershed health assessment tools. Recent research has demonstrated the applicability of such indices for water quality (WQ) constituents such as total suspended solids and nutrients on an individual basis. However, the calculations can become tedious when time-series data for several WQ constituents have to be evaluated individually. Also, comparisons between locations with different sets of constituent data can prove difficult. In this study, data reconstruction using a relevance vector machine algorithm was combined with dimensionality reduction via variational Bayesian noisy principal component analysis to reconstruct and condense sparse multidimensional WQ data sets into a single time series. The methodology allows incorporation of uncertainty in both the reconstruction and dimensionality-reduction steps. The R-R-V values were calculated using the aggregate time series at multiple locations within two Indiana watersheds. Results showed that uncertainty present in the reconstructed WQ data set propagates to the aggregate time series and subsequently to the aggregate R-R-V values as well. This data-driven approach to calculating aggregate R-R-V values was found to be useful for providing a composite picture of watershed health. Aggregate R-R-V values also enabled comparison between locations with different types of WQ data. C1 [Hoque, Yamen M.; Govindaraju, Rao S.] Purdue Univ, Lyles Sch Civil Engn, W Lafayette, IN 47907 USA. [Tripathi, Shivam] Indian Inst Technol, Dept Civil Engn, Kanpur 208016, Uttar Pradesh, India. [Hantush, Mohamed M.] US EPA, Natl Risk Management Res Lab, Off Res & Dev, Cincinnati, OH 45268 USA. [Hoque, Yamen M.] NOAA, Natl Weather Serv, Middle Atlantic River Forecast Ctr, State Coll, PA 16803 USA. RP Hoque, YM (reprint author), Purdue Univ, Lyles Sch Civil Engn, W Lafayette, IN 47907 USA.; Hoque, YM (reprint author), NOAA, Natl Weather Serv, Middle Atlantic River Forecast Ctr, State Coll, PA 16803 USA. EM yamen.hoque@noaa.gov FU USEPA through its Office of Research and Development [EP-C-11-006] FX The USEPA through its Office of Research and Development funded and managed the research described here under USEPA Contract no. EP-C-11-006. It has not been subjected to agency review and therefore does not necessarily reflect the views of the agency, and no official endorsement should be inferred. NR 68 TC 0 Z9 0 U1 11 U2 17 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 709 EP 719 DI 10.2134/jeq2015.10.0508 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900035 PM 27065419 ER PT J AU Verma, S Baig, RBN Nadagouda, MN Varma, RS AF Verma, Sanny Baig, R. B. Nasir Nadagouda, Mallikarjuna N. Varma, Rajender S. TI Selective Oxidation of Alcohols Using Photoactive VO@g-C3N4 SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Photocatalyst; Graphitic carbon nitride; Selective oxidation; Vanadium oxide; Visible light ID GRAPHITIC CARBON NITRIDE; VANADIUM SCHIFF-BASE; C-H ACTIVATION; VISIBLE-LIGHT; AEROBIC OXIDATION; PHOTOREDOX CATALYSIS; MOLECULAR-OXYGEN; GRAPHENE OXIDE; ALDEHYDES; EFFICIENT AB A photoactive VO@g-C3N4 catalyst has been developed for the selective oxidation of alcohols to the corresponding aldehydes and ketones. The visible light mediated activity of the catalyst could be attributed to photoactive graphitic carbon nitrides surface. C1 [Verma, Sanny; Baig, R. B. Nasir; Varma, Rajender S.] US EPA, Sustainable Technol Div, Natl Risk Management Res Lab, MS 443, Cincinnati, OH 45268 USA. [Nadagouda, Mallikarjuna N.] US EPA, WQMB, WSWRD, Natl Risk Management Res Lab, MS 443, Cincinnati, OH 45268 USA. RP Varma, RS (reprint author), US EPA, Sustainable Technol Div, Natl Risk Management Res Lab, MS 443, Cincinnati, OH 45268 USA. EM varma.rajender@epa.gov FU Postgraduate Research Program at the National Risk Management Research Laboratory FX S.V. and R.B.N.B. were supported by the Postgraduate Research Program at the National Risk Management Research Laboratory administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Environmental Protection Agency. The views expressed in this paper are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. Any mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 30 TC 7 Z9 7 U1 19 U2 51 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD MAR PY 2016 VL 4 IS 3 BP 1094 EP 1098 DI 10.1021/acssuschemeng.5b01163 PG 5 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DG0LD UT WOS:000371755400056 ER PT J AU Baig, RBN Verma, S Varma, RS Nadagouda, MN AF Baig, R. B. Nasir Verma, Sanny Varma, Rajender S. Nadagouda, Mallikarjuna N. TI Magnetic Fe@g-C3N4: A Photoactive Catalyst for the Hydrogenation of Alkenes and Alkynes SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Photocatalysis; Hydrogenation; Nanoferrite; Graphitic carbon nitride; Heterogeneous catalysis ID GRAPHITIC CARBON NITRIDE; RETRIEVABLE CATALYSTS; GRAPHENE OXIDE; IRON CATALYSTS; REDUCTION; EFFICIENT; ALCOHOLS; AMINES; COBALT; WATER AB A photoactive catalyst, Fe@g-C3N4, has been developed for the hydrogenation of alkenes and alkynes using hydrazine hydrate as a source of hydrogen. The magnetically separable Fe@g-C3N4 eliminates the use of high pressure hydrogenation, and the reaction can be accomplished using visible light without the need for external sources of energy. C1 [Verma, Sanny; Varma, Rajender S.] US EPA, Sustainable Technol Div, Natl Risk Management Res Lab, MS 443, Cincinnati, OH 45268 USA. [Baig, R. B. Nasir; Nadagouda, Mallikarjuna N.] US EPA, WQMB, WSWRD, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. RP Nadagouda, MN (reprint author), US EPA, WQMB, WSWRD, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. EM nadagouda.mallikarjuna@epa.gov FU Postgraduate Research Program at the National Risk Management Research Laboratory FX N.B.R.B. and S.V. were supported by the Postgraduate Research Program at the National Risk Management Research Laboratory administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Environmental Protection Agency. NR 24 TC 7 Z9 7 U1 31 U2 97 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD MAR PY 2016 VL 4 IS 3 BP 1661 EP 1664 DI 10.1021/acssuschemeng.5b01610 PG 4 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DG0LD UT WOS:000371755400121 ER PT J AU Schroeder, AL Ankley, GT Houck, KA Villeneuve, DL AF Schroeder, Anthony L. Ankley, Gerald T. Houck, Keith A. Villeneuve, Daniel L. TI Environmental surveillance and monitoringThe next frontiers for high-throughput toxicology SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE ToxCast; Tox21; Mixture; Adverse outcome pathway ID EPAS TOXCAST PROGRAM; UPDATE; CHEMICALS; DATABASE; TOXICOGENOMICS; TOXICITY; RISK; RESOURCE; SYSTEM; ACTOR AB High-throughput toxicity testing technologies along with the World Wide Web are revolutionizing both generation of and access to data regarding the biological activities that chemicals can elicit when they interact with specific proteins, genes, or other targets in the body of an organism. To date, however, most of the focus has been on the application of such data to assessment of individual chemicals. The authors suggest that environmental surveillance and monitoring represent the next frontiers for high-throughput toxicity testing. Resources already exist in curated databases of chemical-biological interactions, including highly standardized quantitative dose-response data generated from nascent high-throughput toxicity testing programs such as ToxCast and Tox21, to link chemicals detected through environmental analytical chemistry to known biological activities. The emergence of the adverse outcome pathway framework and the associated knowledge base for linking molecular-level or pathway-level perturbations of biological systems to adverse outcomes traditionally considered in risk assessment and regulatory decision-making through a series of measurable biological changes provides a critical link between activity and hazard. Furthermore, environmental samples can be directly analyzed via high-throughput toxicity testing platforms to provide an unprecedented breadth of biological activity characterization that integrates the effects of all compounds present in a mixture, whether known or not. Novel application of these chemical-biological interaction data provides an opportunity to transform scientific characterization of potential hazards associated with exposure to complex mixtures of environmental contaminants. (c) 2016 SETAC C1 [Schroeder, Anthony L.] Univ Minnesota Twin Cities, Water Resources Ctr, St Paul, MN USA. [Schroeder, Anthony L.; Ankley, Gerald T.; Villeneuve, Daniel L.] US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Duluth, MN USA. [Houck, Keith A.] US EPA, Natl Ctr Computat Toxicol, Off Res & Dev, Res Triangle Pk, NC USA. RP Schroeder, AL (reprint author), Univ Minnesota Twin Cities, Water Resources Ctr, St Paul, MN USA.; Schroeder, AL (reprint author), US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Duluth, MN USA. EM aschroed@umn.edu NR 33 TC 5 Z9 5 U1 10 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD MAR PY 2016 VL 35 IS 3 BP 513 EP 525 DI 10.1002/etc.3309 PG 13 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DF6YY UT WOS:000371505800001 PM 26923854 ER PT J AU Cavallin, JE Jensen, KM Kahl, MD Villeneuve, DL Lee, KE Schroeder, AL Mayasich, J Eid, EP Nelson, KR Milsk, RY Blackwell, BR Berninger, JP LaLone, CA Blanksma, C Jicha, T Elonen, C Johnson, R Ankley, GT AF Cavallin, Jenna E. Jensen, Kathleen M. Kahl, Michael D. Villeneuve, Daniel L. Lee, Kathy E. Schroeder, Anthony L. Mayasich, Joe Eid, Evan P. Nelson, Krysta R. Milsk, Rebecca Y. Blackwell, Brett R. Berninger, Jason P. LaLone, Carlie A. Blanksma, Chad Jicha, Terri Elonen, Colleen Johnson, Rodney Ankley, Gerald T. TI PATHWAY-BASED APPROACHES FOR ASSESSMENT OF REAL-TIME EXPOSURE TO AN ESTROGENIC WASTEWATER TREATMENT PLANT EFFLUENT ON FATHEAD MINNOW REPRODUCTION SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Wastewater treatment plant; Fish; Reproduction; Estrogens ID ENDOCRINE-DISRUPTING CHEMICALS; ACTIVATION ASSAY T47D-KBLUC; PIMEPHALES-PROMELAS; IN-VITRO; METABOLIZING ENZYMES; PIPERONYL BUTOXIDE; STEROID ESTROGENS; STABLY EXPRESSES; OXIDATIVE STRESS; GENE-EXPRESSION AB Wastewater treatment plant (WWTP) effluents are known contributors of chemical mixtures into the environment. Of particular concern are endocrine-disrupting compounds, such as estrogens, which can affect the hypothalamic-pituitary-gonadal axis function in exposed organisms. The present study examined reproductive effects in fathead minnows exposed for 21 d to a historically estrogenic WWTP effluent. Fathead minnow breeding pairs were held in control water or 1 of 3 effluent concentrations (5%, 20%, and 100%) in a novel onsite, flow-through system providing real-time exposure. The authors examined molecular and biochemical endpoints representing key events along adverse outcome pathways linking estrogen receptor activation and other molecular initiating events to reproductive impairment. In addition, the authors used chemical analysis of the effluent to construct a chemical-gene interaction network to aid in targeted gene expression analyses and identifying potentially impacted biological pathways. Cumulative fecundity was significantly reduced in fish exposed to 100% effluent but increased in those exposed to 20% effluent, the approximate dilution factor in the receiving waters. Plasma vitellogenin concentrations in males increased in a dose-dependent manner with effluent concentration; however, male fertility was not impacted. Although in vitro analyses, analytical chemistry, and biomarker responses confirmed the effluent was estrogenic, estrogen receptor agonists were unlikely the primary driver of impaired reproduction. The results provide insights into the significance of pathway-based effects with regard to predicting adverse reproductive outcomes. Published 2015 by Wiley Periodicals Inc. on behalf of SETAC. This article is a US Government work, and as such, is in the public domain in the United States of America. C1 [Cavallin, Jenna E.; Milsk, Rebecca Y.; Blackwell, Brett R.] US EPA, ORISE Res Participat Program, Off Res & Dev, Natl Hlth & Environm Effects Res Lab,Midcontinent, Duluth, MN USA. [Cavallin, Jenna E.] Univ Minnesota, Integrated Biosci Grad Program, Duluth, MN 55812 USA. [Jensen, Kathleen M.; Kahl, Michael D.; Villeneuve, Daniel L.; Eid, Evan P.; Nelson, Krysta R.; Berninger, Jason P.; LaLone, Carlie A.; Jicha, Terri; Elonen, Colleen; Johnson, Rodney; Ankley, Gerald T.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Midcontinent Ecol Div, Duluth, MN USA. [Lee, Kathy E.] US Geol Survey, Tox Subst Hydrol Program, Grand Rapids, MI USA. [Schroeder, Anthony L.] Univ Minnesota, Water Resources Ctr, Natl Hlth & Environm Effects Res Lab,Midcontinent, US Environm Protect Agcy,Off Res & Dev, Duluth, MN 55812 USA. [Mayasich, Joe] Western Lake Super Sanit Dist, Duluth, MN USA. [Blanksma, Chad] US EPA, Badger Tech Serv, Off Res & Dev, Natl Hlth & Environm Effects Res Lab,Mid Continen, Duluth, MN USA. RP Cavallin, JE (reprint author), US EPA, ORISE Res Participat Program, Off Res & Dev, Natl Hlth & Environm Effects Res Lab,Midcontinent, Duluth, MN USA.; Cavallin, JE (reprint author), Univ Minnesota, Integrated Biosci Grad Program, Duluth, MN 55812 USA. EM cavallin.jenna@epa.gov RI Berninger, Jason/O-2401-2016 OI Berninger, Jason/0000-0003-3045-7899 FU University of Minnesota-US Environmental Protection Agency Cooperative Training Partnership FX We thank the staff and management at the Western Lake Superior Sanitary District. Additional technical support was provided by A. Parrella (Western Lake Superior Sanitary District), K. Lott (Badger Technical Services), F. Whiteman, M. Lee, M. Hughes, E. Randolph, T. Saari (USEPA), and S. Robinson (USEPA GRO intern). We also thank members of the US Geological Survey National Water Quality Laboratory, who conducted the analytical measurements of the water samples and S. Langer for assistance with water sample coordination. We thank D. Mount for reviewing an earlier draft of the present study. J. Cavallin was supported in part by the University of Minnesota-US Environmental Protection Agency Cooperative Training Partnership. NR 90 TC 1 Z9 1 U1 9 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD MAR PY 2016 VL 35 IS 3 BP 702 EP 716 DI 10.1002/etc.3228 PG 15 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DF6YY UT WOS:000371505800019 PM 26332155 ER PT J AU Consoer, DM Hoffman, AD Fitzsimmons, PN Kosian, PA Nichols, JW AF Consoer, Daniel M. Hoffman, Alex D. Fitzsimmons, Patrick N. Kosian, Patricia A. Nichols, John W. TI TOXICOKINETICS OF PERFLUOROOCTANE SULFONATE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Perfluorooctane sulfonate; Toxicokinetics; Renal elimination; Tissue distribution; Bioaccumulation ID CHAIN PERFLUORINATED ACIDS; ORGANIC ANION TRANSPORTER; TANDEM MASS-SPECTROMETRY; HUMAN SERUM-ALBUMIN; TISSUE DISTRIBUTION; PERFLUOROALKYL ACIDS; P-GLYCOPROTEIN; EFFLUX TRANSPORTERS; AQUATIC ORGANISMS; WINTER FLOUNDER AB Rainbow trout (Oncorhynchus mykiss) confined to respirometer-metabolism chambers were dosed with perfluorooctane sulfonate (PFOS) by intra-arterial injection and sampled to obtain concentration time-course data for plasma and either urine or expired water. The data were then analyzed using a 2-compartment clearance-volume model. Renal and branchial clearance rates (mL/d/kg) determined for all experiments averaged 19% and 81% of total clearance, respectively. Expressed as mean values for all experiments, the steady-state volume of distribution was 277mL/kg and the terminal half-life was 86.8 d. Additional animals were exposed to PFOS in water, resulting in an average calculated branchial uptake efficiency of 0.36%. The renal clearance rate determined in the present study is approximately 75 times lower than that determined in earlier studies with perfluorooctanoate (PFOA). Previously, it was suggested that PFOA is a substrate for membrane transporters in the trout kidney. The present study suggests that glomerular filtration may be sufficient to explain the observed renal clearance rate for PFOS, although a role for membrane transporters cannot be ruled out. These findings demonstrate that models developed to predict the bioaccumulation of perfluoroalkyl acids by fish must account for differences in renal clearance of individual compounds. (c) 2015 SETAC C1 [Consoer, Daniel M.; Hoffman, Alex D.; Fitzsimmons, Patrick N.; Kosian, Patricia A.; Nichols, John W.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Mid Continent Ecol Div, Duluth, MN USA. [Consoer, Daniel M.] Univ Minnesota, Dept Toxicol, Duluth, MN 55812 USA. RP Nichols, JW (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Mid Continent Ecol Div, Duluth, MN USA. EM nichols.john@epa.gov NR 64 TC 0 Z9 0 U1 7 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD MAR PY 2016 VL 35 IS 3 BP 717 EP 727 DI 10.1002/etc.3230 PG 11 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DF6YY UT WOS:000371505800020 PM 26332333 ER PT J AU Raimondo, S Lilavois, CR Lee, L Augspurger, T Wang, N Ingersoll, CG Bauer, C Hammer, E Barron, MG AF Raimondo, Sandy Lilavois, Crystal R. Lee, Larisa Augspurger, Tom Wang, Ning Ingersoll, Chris G. Bauer, Candice Hammer, Edward Barron, Mace G. TI Assessing variability in chemical acute toxicity of unionid mussels: Influence of intra- and interlaboratory testing, life stage, and species SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Mussels; Freshwater toxicity; Life stages; Species extrapolation; Variability ID FRESH-WATER MUSSELS; LAMPSILIS-SILIQUOIDEA; ESTIMATION MODELS; GLOCHIDIA; JUVENILES; SENSITIVITY; AMMONIA; COPPER; MOLLUSKS AB The authors developed a toxicity database for unionid mussels to examine the extent of intra- and interlaboratory variability in acute toxicity tests with mussel larvae (glochidia) and juveniles; the extent of differential sensitivity of the 2 life stages; and the variation in sensitivity among commonly tested mussels (Lampsilis siliquoidea, Utterbackia imbecillis, and Villosa iris), commonly tested cladocerans (Daphnia magna and Ceriodaphnia dubia), and fish (Oncorhynchus mykiss, Pimephales promelas, and Lepomis macrochirus). The results of these analyses indicate that intralaboratory variability for median effect concentrations (EC50) averaged about 2-fold for both life stages, whereas interlaboratory variability averaged 3.6-fold for juvenile mussels and 6.3-fold for glochidia. The EC50s for juveniles and glochidia were within a factor of 2 of each other for 50% of paired records across chemicals, with juveniles more sensitive than glochidia by more than 2-fold for 33% of the comparisons made between life stages. There was a high concurrence of sensitivity of commonly tested L. siliquoidea, U. imbecillis, and V. iris to that of other mussels. However, this concurrence decreased as the taxonomic distance of the commonly tested cladocerans and fish to mussels increased. The compiled mussel database and determination of data variability will advance risk assessments by including more robust species sensitivity distributions, interspecies correlation estimates, and availability of taxon-specific empirically derived application factors for risk assessment. Environ Toxicol Chem 2016;35:750-758. Published 2015 Wiley Periodicals Inc on behalf of SETAC. This article is a US Government work and, as such, is in the public domain in the United States of America. C1 [Raimondo, Sandy; Lilavois, Crystal R.; Lee, Larisa; Barron, Mace G.] US EPA, Gulf Ecol Div, Gulf Breeze, FL USA. [Augspurger, Tom] US Fish & Wildlife Serv, Raleigh, NC USA. [Wang, Ning; Ingersoll, Chris G.] US Geol Survey, Columbia, MO USA. [Bauer, Candice; Hammer, Edward] US EPA, Chicago, IL USA. RP Raimondo, S (reprint author), US EPA, Gulf Ecol Div, Gulf Breeze, FL USA. EM Raimondo.sandy@epa.gov NR 40 TC 3 Z9 3 U1 3 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD MAR PY 2016 VL 35 IS 3 BP 750 EP 758 DI 10.1002/etc.3245 PG 9 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DF6YY UT WOS:000371505800024 PM 26369904 ER PT J AU Zheng, Y Alapaty, K Herwehe, JA Del Genio, AD Niyogi, D AF Zheng, Yue Alapaty, Kiran Herwehe, Jerold A. Del Genio, Anthony D. Niyogi, Dev TI Improving High-Resolution Weather Forecasts Using the Weather Research and Forecasting (WRF) Model with an Updated Kain-Fritsch Scheme SO MONTHLY WEATHER REVIEW LA English DT Article DE Forecasting; Numerical weather prediction/forecasting; Hindcasts; Forecasting; Operational forecasting ID NONHYDROSTATIC ATMOSPHERIC MODEL; CONVECTIVE PARAMETERIZATION; PART I; CUMULUS PARAMETERIZATION; HORIZONTAL RESOLUTION; CLIMATE SIMULATIONS; MESOSCALE MODEL; TIME-SCALE; PRECIPITATION; SENSITIVITY AB Efforts to improve the prediction accuracy of high-resolution (1-10 km) surface precipitation distribution and variability are of vital importance to local aspects of air pollution, wet deposition, and regional climate. However, precipitation biases and errors can occur at these spatial scales due to uncertainties in initial meteorological conditions and/or grid-scale cloud microphysics schemes. In particular, it is still unclear to what extent a subgrid-scale convection scheme could be modified to bring in scale awareness for improving high-resolution short-term precipitation forecasts in the WRF Model. To address these issues, the authors introduced scale-aware parameterized cloud dynamics for high-resolution forecasts by making several changes to the Kain-Fritsch (KF) convective parameterization scheme in the WRF Model. These changes include subgrid-scale cloud-radiation interactions, a dynamic adjustment time scale, impacts of cloud updraft mass fluxes on grid-scale vertical velocity, and lifting condensation level-based entrainment methodology that includes scale dependency. A series of 48-h retrospective forecasts using a combination of three treatments of convection (KF, updated KF, and the use of no cumulus parameterization), two cloud microphysics schemes, and two types of initial condition datasets were performed over the U.S. southern Great Plains on 9- and 3-km grid spacings during the summers of 2002 and 2010. Results indicate that 1) the source of initial conditions plays a key role in high-resolution precipitation forecasting, and 2) the authors' updated KF scheme greatly alleviates the excessive precipitation at 9-km grid spacing and improves results at 3-km grid spacing as well. Overall, the study found that the updated KF scheme incorporated into a high-resolution model does provide better forecasts for precipitation location and intensity. C1 [Zheng, Yue; Niyogi, Dev] Purdue Univ, Dept Earth Atmospher & Planetary Sci, W Lafayette, IN 47907 USA. [Alapaty, Kiran; Herwehe, Jerold A.] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Del Genio, Anthony D.] NASA, Goddard Inst Space Studies, New York, NY 10025 USA. [Niyogi, Dev] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. RP Alapaty, K (reprint author), US EPA, Mail Code E243-01, Res Triangle Pk, NC 27711 USA. EM alapaty.kiran@epa.gov FU U.S. EPA's Air, Climate, and Energy (ACE) Program; USDA/NIFA Drought Triggers through Texas AM University [2011-67019-20042]; NSF [AGS-1522494, CDSE-1250232]; USDA NIFA Hatch Project at Purdue University [1007699]; U.S. Department of Energy Atmospheric System Research Program FX Parts of the research were funded by the U.S. EPA's Air, Climate, and Energy (ACE) Program, and USDA/NIFA Drought Triggers Grant 2011-67019-20042 through Texas A&M University, NSF Grants AGS-1522494 and CDS&E-1250232, and USDA NIFA Hatch Project 1007699 at Purdue University. Anthony Del Genio acknowledges support from the U.S. Department of Energy Atmospheric System Research Program. Our appreciation goes to Dr. John Kain of NOAA and Dr. Megan Mallard, Mr. Russell Bullock, Dr. Christopher Nolte, and Ms. Tanya Spero of the U.S. EPA for their help in many ways facilitating the research. This research has been subjected to the U.S. EPA's administrative review and approved for publication. The views expressed herein and the contents are solely the responsibility of the authors, and do not necessarily represent the official views of the U.S. EPA. NR 81 TC 8 Z9 8 U1 3 U2 11 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 0027-0644 EI 1520-0493 J9 MON WEATHER REV JI Mon. Weather Rev. PD MAR PY 2016 VL 144 IS 3 BP 833 EP 860 DI 10.1175/MWR-D-15-0005.1 PG 28 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DF6PL UT WOS:000371477600001 ER PT J AU McCullough, SD Bowers, EC On, DM Morgan, DS Dailey, LA Hines, RN Devlin, RB Diaz-Sanchez, D AF McCullough, Shaun D. Bowers, Emma C. On, Doan M. Morgan, David S. Dailey, Lisa A. Hines, Ronald N. Devlin, Robert B. Diaz-Sanchez, David TI Baseline Chromatin Modification Levels May Predict Interindividual Variability in Ozone-Induced Gene Expression SO TOXICOLOGICAL SCIENCES LA English DT Article DE chromatin; DNA methylation; histone; ozone; epigenetics ID NUCLEOSOME CORE PARTICLE; AIRWAY EPITHELIAL-CELLS; EMBRYONIC STEM-CELLS; ANGSTROM RESOLUTION; EPIGENETICS; EXPOSURE; 5-HYDROXYMETHYLCYTOSINE; DNA; DIFFERENTIATION; REPRODUCIBILITY AB Traditional toxicological paradigms have relied on factors such as age, genotype, and disease status to explain variability in responsiveness to toxicant exposure; however, these are neither sufficient to faithfully identify differentially responsive individuals nor are they modifiable factors that can be leveraged to mitigate the exposure effects. Unlike these factors, the epigenome is dynamic and shaped by an individual's environment. We sought to determine whether baseline levels of specific chromatin modifications correlated with the interindividual variability in their ozone (O-3)-mediated induction in an air-liquid interface model using primary human bronchial epithelial cells from a panel of 11 donors. We characterized the relationship between the baseline abundance of 6 epigenetic markers with established roles as key regulators of gene expression-histone H3 lysine 4 trimethylation (H3K4me3), H3K27 acetylation (H3K27ac), pan-acetyl H4 (H4ac), histone H3K27 di/trimethylation (H3K27me2/3), unmodified H3, and 5-hydroxymethylcytosine (5-hmC)-and the variability in the O-3-induced expression of IL-8, IL-6, COX2, and HMOX1. Baseline levels of H3K4me3, H3K27me2/3, and 5-hmC, but not H3K27ac, H4ac, and total H3, correlated with the interindividual variability in O-3-mediated induction of HMOX1 and COX2. In contrast, none of the chromatin modifications that we examined correlated with the induction of IL-8 and IL-6. From these findings, we propose an "epigenetic seed and soil" model in which chromatin modification states between individuals differ in the relative abundance of specific modifications (the "soil") that govern how receptive the gene is to toxicant-mediated cellular signals (the "seed") and thus regulate the magnitude of exposure-related gene induction. C1 [McCullough, Shaun D.; On, Doan M.; Morgan, David S.; Dailey, Lisa A.; Hines, Ronald N.; Devlin, Robert B.; Diaz-Sanchez, David] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. [Bowers, Emma C.] Univ N Carolina, Curriculum Toxicol, Chapel Hill, NC 27599 USA. RP McCullough, SD (reprint author), US EPA, Human Studies Facil, Natl Hlth & Environm Effects Res Lab, Environm Publ Hlth Div, 104 Mason Farm Rd, Chapel Hill, NC 27599 USA. EM mccullough.shaun@epa.gov FU Office of Research and Development's Pathfinder Innovation Program, a U.S. Environmental Protection Agency intramural funding program FX This work was supported by the Office of Research and Development's Pathfinder Innovation Program, a U.S. Environmental Protection Agency intramural funding program. NR 37 TC 3 Z9 3 U1 3 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD MAR PY 2016 VL 150 IS 1 BP 216 EP 224 DI 10.1093/toxsci/kfv324 PG 9 WC Toxicology SC Toxicology GA DF8NS UT WOS:000371615300019 PM 26719369 ER PT J AU Garrick, M Ghio, A Zhao, L Koury, ST Wolff, NA Thevenod, F Chowdhury, S Cutts, AB Cadieux, JA Goldberg, YP Cohen, C Collins, JF Garrick, LM AF Garrick, Michael Ghio, Andrew Zhao, Lin Koury, Stephen T. Wolff, Natascha A. Thevenod, Frank Chowdhury, Sultan Cutts, Alison B. Cadieux, Jay A. Goldberg, Y. Paul Cohen, Charles Collins, James F. Garrick, Laura M. TI FUNCTIONAL PROPERTIES OF DIVALENT METAL TRANSPORTER 1 (DMT1) ON THE OUTER MITOCHONDRIAL MEMBRANE (OMM) SO AMERICAN JOURNAL OF HEMATOLOGY LA English DT Meeting Abstract C1 [Garrick, Michael; Zhao, Lin; Koury, Stephen T.; Garrick, Laura M.] SUNY Buffalo, Buffalo, NY 14260 USA. [Ghio, Andrew] US EPA, New York, NY USA. [Wolff, Natascha A.; Thevenod, Frank] Univ Witten Herdecke, Witten, Germany. [Chowdhury, Sultan; Cutts, Alison B.; Cadieux, Jay A.; Goldberg, Y. Paul; Cohen, Charles] Xenon Pharmaceut, Vancouver, BC, Canada. [Collins, James F.] Univ Florida, Gainesville, FL 32611 USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0361-8609 EI 1096-8652 J9 AM J HEMATOL JI Am. J. Hematol. PD MAR PY 2016 VL 91 IS 3 MA 53 BP E73 EP E73 PG 1 WC Hematology SC Hematology GA DF2HI UT WOS:000371162800060 ER PT J AU Gordon, CJ Phillips, PM Johnstone, AFM AF Gordon, Christopher J. Phillips, Pamela M. Johnstone, Andrew F. M. TI A noninvasive method to study regulation of extracellular fluid volume in rats using nuclear magnetic resonance SO AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY LA English DT Article DE fluid homeostasis; rodent; noninvasive; unanesthetized; deydration ID PERITONEAL MEMBRANE; SOLUTE TRANSPORT; BODY-COMPOSITION; PLASMA-VOLUME; EXERCISE; WATER; ULTRAFILTRATION; DEHYDRATION; OSMOLALITY; INCREASES AB Timedomain nuclear magnetic resonance (TD-NMR)-based measurement of body composition of rodents is an effective method to quickly and repeatedly measure proportions of fat, lean, and fluid without anesthesia. TD-NMR provides a measure of free water in a living animal, termed % fluid, and is a measure of unbound water in the vascular and extracellular spaces. We hypothesized that injecting a bolus of fluid into the peritoneal cavity would lead to an abrupt increase in % fluid and the rate of clearance monitored with TD-NMR would provide a noninvasive assessment of the free water homeostasis in an awake rat. Several strains of laboratory rats were injected intraperitoneally with 10 ml/kg isotonic or hypertonic saline and % fluid was monitored repeatedly with a Bruker "Minispec" TD-NMR body composition system. Following isotonic saline, % fluid increased immediately by 0.5% followed by a recovery over similar to 6 h. Injecting hypertonic (3 times normal saline) resulted in a significantly greater rise in % fluid and longer recovery. Intraperitoneal and subcutaneous fluid injection led to similar rates of clearance. The Wistar-Kyoto rat strain displayed significantly slower recovery to fluid loads compared with Long-Evans and Sprague-Dawley strains. Rats exercised chronically showed significant increases in % fluid, but the rate of clearance of fluid was similar to that of sedentary animals. We conclude that this technique could be used to study vascular and extracellular volume homeostasis noninvasively in rats. C1 [Gordon, Christopher J.; Phillips, Pamela M.; Johnstone, Andrew F. M.] US EPA, Tox Assessment Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. RP Gordon, CJ (reprint author), US EPA, Tox Assessment Div, Res Triangle Pk, NC 27711 USA. EM Gordon.christopher@epa.gov NR 19 TC 1 Z9 1 U1 1 U2 2 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 1931-857X EI 1522-1466 J9 AM J PHYSIOL-RENAL JI Am. J. Physiol.-Renal Physiol. PD MAR 1 PY 2016 VL 310 IS 5 BP F426 EP F431 DI 10.1152/ajprenal.00405.2015 PG 6 WC Physiology; Urology & Nephrology SC Physiology; Urology & Nephrology GA DF4FX UT WOS:000371304500010 PM 26697983 ER PT J AU Shen, GF AF Shen, Guofeng TI Changes from traditional solid fuels to clean household energies - Opportunities in emission reduction of primary PM2.5 from residential cookstoves in China SO BIOMASS & BIOENERGY LA English DT Article DE Traditional solid fuels; Clean household energies; Primary PM2.5; Emission reduction; Residential cookstoves ID POLYCYCLIC AROMATIC-HYDROCARBONS; CARBONACEOUS PARTICULATE MATTER; INDOOR AIR-POLLUTION; BIOFUEL COMBUSTION; RURAL CHINA; GASEOUS-POLLUTANTS; DOMESTIC BIOMASS; COAL COMBUSTION; WOOD COMBUSTION; CLIMATE-CHANGE AB Household biomass and coal use is an important source of PM2.5 emission in China due to low efficient burnings and large consumptions. Primary PM2.5 emissions for different fuels are compared based on a compilation of data from emission measurements of Chinese cookstoves in literature. The burning of pellets had low PM2.5 emissions at 0.42 and 0.18 g MJ(d)(-1) for straw and wood pellets, respectively. There would be an emission reduction of about 80% compared to ordinary raw biomass fuel burning for cooking. The average emissions of PM2.5 per useful energy delivered were 0.10 and 0.28 g MJ(d)(-1) anthracite briquette and chunk, but as high as 1.2 and 3.2 g MJ(d)(-1) for bituminous briquette and chunk, respectively. Coals burned in the form of briquette may lower PM2.5 emission by 60% compared to the burning of raw chunk. The adoption of improved high efficiency cookstoves would have a comparable reduction in primary emissions compared to a fuel switch. Gas should be promoted for cooking as primary PM2.5 emissions are 1,2 orders of magnitude lower compared to those for solid fuels. More emission measurements and fuel consumption survey are needed to fill data gap and to support interventions of advanced fuel-stove combinations. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Shen, Guofeng] Jiangsu Acad Environm Sci, Jiangsu Prov Key Lab Environm Engn, Nanjing 210036, Jiangsu, Peoples R China. [Shen, Guofeng] US EPA, Natl Risk Management Res Lab, RTP, Flint, MI 48501 USA. RP Shen, GF (reprint author), Jiangsu Acad Environm Sci, Jiangsu Prov Key Lab Environm Engn, Nanjing 210036, Jiangsu, Peoples R China.; Shen, GF (reprint author), US EPA, Natl Risk Management Res Lab, RTP, Flint, MI 48501 USA. EM gfshen12@gmail.com RI SHEN, Guofeng/F-3163-2015 OI SHEN, Guofeng/0000-0002-7731-5399 FU Jiangsu Natural Science Foundation [BK20131031] FX I'd like to acknowledge the editor and anonymous reviewers for detailed and valuable comments and suggestions to improve the manuscript, and Dr. Ebersviller for polishing the language. The funding of this study is partly supported by Jiangsu Natural Science Foundation (BK20131031). NR 66 TC 4 Z9 5 U1 13 U2 48 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD MAR PY 2016 VL 86 BP 28 EP 35 DI 10.1016/j.biombioe.2016.01.004 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DF4PN UT WOS:000371331800004 ER PT J AU Rager, JE Strynar, MJ Liang, S McMahen, RL Richard, AM Grulke, CM Wambaugh, JF Isaacs, KK Judson, R Williams, AJ Sobus, JR AF Rager, Julia E. Strynar, Mark J. Liang, Shuang McMahen, Rebecca L. Richard, Ann M. Grulke, Christopher M. Wambaugh, John F. Isaacs, Kristin K. Judson, Richard Williams, Antony J. Sobus, Jon R. TI Linking high resolution mass spectrometry data with exposure and toxicity forecasts to advance high-throughput environmental monitoring SO ENVIRONMENT INTERNATIONAL LA English DT Article DE Non-targeted; Suspect screening; Exposome; ExpoCast; ToxCast; Dust ID IN-HOUSE DUST; ARYL-HYDROCARBON RECEPTOR; BIOLOGICAL SAMPLES; FLAME RETARDANTS; INDOOR DUST; PPAR-GAMMA; PRIORITIZATION; CHEMICALS; CONTAMINANTS; DISEASE AB There is a growing need in the field of exposure science for monitoring methods that rapidly screen environmental media for suspect contaminants. Measurement and analysis platforms, based on high resolution mass spectrometry (HRMS), now exist to meet this need. Here we describe results of a study that links HRMS data with exposure predictions from the U.S. EPA's ExpoCast (TM) program and in vitro bioassay data from the U.S. interagency Tox21 consortium. Vacuum dust samples were collected from 56 households across the U.S. as part of the American Healthy Homes Survey (AHHS). Sample extracts were analyzed using liquid chromatography time-of-flight mass spectrometry (LC-TOF/MS) with electrospray ionization. On average, approximately 2000 molecular features were identified per sample (based on accurate mass) in negative ion mode, and 3000 in positive ion mode. Exact mass, isotope distribution, and isotope spacing were used to match molecular features with a unique listing of chemical formulas extracted from EPA's Distributed Structure-Searchable Toxicity (DSSTox) database. A total of 978 DSSTox formulas were consistent with the dust LC-TOF/molecular feature data (match score >= 90); these formulas mapped to 3228 possible chemicals in the database. Correct assignment of a unique chemical to a given formula required additional validation steps. Each suspect chemical was prioritized for follow-up confirmation using abundance and detection frequency results, along with exposure and bioactivity estimates from ExpoCast and Tox21, respectively. Chemicals with elevated exposure and/or toxicity potential were further examined using a mixture of 100 chemical standards. A total of 33 chemicals were confirmed present in the dust samples by formula and retention time match; nearly half of these do not appear to have been associated with house dust in the published literature. Chemical matches found in at least 10 of the 56 dust samples include Piperine, N,N-Diethyl-m-toluamide (DEET), Triclocarban, Diethyl phthalate (DEP), Propylparaben, Methylparaben, Tris(1,3-dichloro-2-propyl)phosphate (TDCPP), and Nicotine. This study demonstrates a novel suspect screening methodology to prioritize chemicals of interest for subsequent targeted analysis. The methods described here rely on strategic integration of available public resources and should be considered in future non-targeted and suspect screening assessments of environmental and biological media. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Rager, Julia E.; Liang, Shuang; McMahen, Rebecca L.] 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Strynar, Mark J.; Isaacs, Kristin K.; Sobus, Jon R.] US EPA, Off Res & Dev, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Richard, Ann M.; Wambaugh, John F.; Judson, Richard; Williams, Antony J.] US EPA, Off Res & Dev, Natl Ctr Computat Toxicol, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Grulke, Christopher M.] Lockheed Martin, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. RP Sobus, JR (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM sobus.jon@epa.gov OI Wambaugh, John/0000-0002-4024-534X FU United States Environmental Protection Agency through Office of Research and Development FX The United States Environmental Protection Agency through its Office of Research and Development funded and managed the research described here. It has been subjected to Agency administrative review and approved for publication. Julia Rager, Shuang Liang, and Rebecca McMahen were supported by an appointment to the Internship/Research Participation Program at the Office of Research and Development, U.S. Environmental Protection Agency, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. NR 54 TC 11 Z9 11 U1 17 U2 51 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD MAR PY 2016 VL 88 BP 269 EP 280 DI 10.1016/j.envint.2015.12.008 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA DF4YU UT WOS:000371359300034 PM 26812473 ER PT J AU Albertson, JD Harvey, T Foderaro, G Zhu, PP Zhou, XC Ferrari, S Amin, MS Modrak, M Brantley, H Thoma, ED AF Albertson, John. D. Harvey, Tierney Foderaro, Greg Zhu, Pingping Zhou, Xiaochi Ferrari, Silvia Amin, M. Shahrooz Modrak, Mark Brantley, Halley Thoma, Eben D. TI A Mobile Sensing Approach for Regional Surveillance of Fugitive Methane Emissions in Oil and Gas Production SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID BARNETT SHALE REGION; COMPRESSOR STATIONS; PRODUCTION SITES; PIPELINE LEAKS; UNITED-STATES; OPERATIONS; FACILITIES AB This paper addresses the need for surveillance of fugitive methane emissions over broad geographical regions. Most existing techniques suffer from being either extensive (but qualitative) or quantitative (but intensive with poor scalability). A total of two novel advancements are made here. First, a recursive Bayesian method is presented for probabilistically characterizing fugitive point-sources from mobile sensor data. This approach is made possible by a new cross-plume integrated dispersion formulation that overcomes much of the need for time-averaging concentration data. The method is tested here against a limited data set of controlled methane release and shown to perform well. We then present an information-theoretic approach to plan the paths of the sensor equipped vehicle, where the path is chosen so as to maximize expected reduction in integrated target source rate uncertainty in the region, subject to given starting and ending positions and prevailing meteorological conditions. The information-driven sensor path planning algorithm is tested and shown to provide robust results across a wide range of conditions. An overall system concept is presented for optionally piggybacking of these techniques onto normal industry maintenance operations using sensor equipped work trucks. C1 [Albertson, John. D.] Cornell Univ, Sch Civil & Environm Engn, Ithaca, NY 14853 USA. [Foderaro, Greg; Zhu, Pingping; Ferrari, Silvia] Cornell Univ, Sibley Sch Mech & Aerosp Engn, Ithaca, NY 14853 USA. [Albertson, John. D.; Harvey, Tierney; Zhou, Xiaochi] Duke Univ, Dept Civil & Environm Engn, Durham, NC 27708 USA. [Ferrari, Silvia] Duke Univ, Dept Mech Engn & Mat Sci, Durham, NC 27708 USA. [Amin, M. Shahrooz; Modrak, Mark] Arcadis US Inc, 4915 Prospectus Dr F, Durham, NC 27713 USA. [Brantley, Halley; Thoma, Eben D.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, 109 TW Alexander Dr,E343-02, Res Triangle Pk, NC 27711 USA. [Harvey, Tierney] Univ Cent Oklahoma, Dept Engn & Phys, Edmond, OK 73034 USA. [Foderaro, Greg] Appl Res Associates Inc, 8537 Six Forks Rd, Raleigh, NC 27615 USA. [Amin, M. Shahrooz] NIMA Grp LLC, 4700 Rochester Rd, Troy, MI 48085 USA. [Modrak, Mark] Aecom Technol Corp, 9400 Amberglen Blvd, Austin, TX 78729 USA. RP Albertson, JD (reprint author), Cornell Univ, Sch Civil & Environm Engn, Ithaca, NY 14853 USA.; Albertson, JD (reprint author), Duke Univ, Dept Civil & Environm Engn, Durham, NC 27708 USA. EM albertson@cornell.edu FU NSF IGERT [DGE-1068871]; U.S. EPA ORDs Air, Climate, and Energy (ACE) program FX This work was supported by the NSF IGERT through Grant DGE-1068871 and U.S. EPA ORDs Air, Climate, and Energy (ACE) program. The authors thank Bill Squire and Bill Mitchell from EPA for their efforts on this project. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 37 TC 1 Z9 1 U1 6 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD MAR 1 PY 2016 VL 50 IS 5 BP 2487 EP 2497 DI 10.1021/acs.est.5b05059 PG 11 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DF5DJ UT WOS:000371371700042 PM 26807713 ER PT J AU Boczek, LA Rhodes, ER Cashdollar, JL Ryu, J Popovici, J Hoelle, JM Sivaganesan, M Hayes, SL Rodgers, MR Ryu, H AF Boczek, Laura A. Rhodes, Eric R. Cashdollar, Jennifer L. Ryu, Jongseong Popovici, Jonathan Hoelle, Jill M. Sivaganesan, Mano Hayes, Samuel L. Rodgers, Mark R. Ryu, Hodon TI Applicability of UV resistant Bacillus pumilus endospores as a human adenovirus surrogate for evaluating the effectiveness of virus inactivation in low-pressure UV treatment systems SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Low-pressure UV disinfection; UV resistant Bacillus pumilus endospores human adenovirus surrogate ID PCR; SPORES; LIGHT; QPCR AB Recent studies have demonstrated the potential to use Bacillus pumilus endospores as a surrogate for human adenovirus (HAdV) in UV disinfection studies. The use of endospores has been limited by observations of batch-to-batch variation in UV sensitivity. This study reports on a propagation method that utilizes a commercially available medium to produce UV tolerant B. pumilus endospores with a consistent UV sensitivity. It is further demonstrated that the endospores of B. pumilus strain (ATCC 27142), produced using this protocol (half strength Columbia broth, 5 days incubation, with 0.1 mM MnSO4), display a UV dose-response that is similar to that of HAdV. Endospore stocks could be stored in ethanol for up to two months at 4 degrees C without a significant change in DV sensitivity. Synergistic endospore damage was observed by pre-heat treatment of water samples followed by UV irradiation. UV tolerant B. pumilus endospores are a potential surrogate of HAdV for UV treatment performance tests in water utilities which do not have in-house research virology laboratories. Published by Elsevier B.V. C1 [Boczek, Laura A.; Popovici, Jonathan; Hoelle, Jill M.; Sivaganesan, Mano; Hayes, Samuel L.; Rodgers, Mark R.; Ryu, Hodon] US EPA, Off Res & Dev, Natl Risk Management Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. [Rhodes, Eric R.; Cashdollar, Jennifer L.] US EPA, Off Res & Dev, Natl Exposure Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. [Ryu, Jongseong] Anyang Univ, Dept Marine Biotechnol, 602-14 Jungang Ro, Inchon 417833, South Korea. RP Ryu, H (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA. EM ryu.hodon@epa.gov FU U.S. Environmental Protection Agency through Office of Research and Development FX We thank Jenifer Jones, Amanda Jennings, and Emma Huff for technical assistance. We also appreciate Dr. Eugene Rice for the critical review of the manuscript. The U.S. Environmental Protection Agency, through its Office of Research and Development, funded and managed the research described herein. This work has been subjected to the agency's administrative review and has been approved for external publication. Any opinions expressed in this paper are those of the authors and do not necessarily reflect the views of the agency; therefore, no official endorsement should be inferred. Any mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 23 TC 1 Z9 1 U1 2 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD MAR PY 2016 VL 122 BP 43 EP 49 DI 10.1016/j.mimet.2016.01.012 PG 7 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA DF5CA UT WOS:000371368200010 PM 26825005 ER PT J AU Pinto, PX Al-Abed, SR Balz, DA Butler, BA Landy, RB Smith, SJ AF Pinto, Patricio X. Al-Abed, Souhail R. Balz, David A. Butler, Barbara A. Landy, Ronald B. Smith, Samantha J. TI Bench-Scale and Pilot-Scale Treatment Technologies for the Removal of Total Dissolved Solids from Coal Mine Water: A Review SO MINE WATER AND THE ENVIRONMENT LA English DT Review DE Adsorption; Bioremediation; Desalination; Distillation; Ion exchange; Precipitation; Reverse osmosis ID SULFATE-REDUCING BACTERIA; LOW-COST ADSORBENTS; HEAVY-METAL IONS; METHYLENE PHOSPHONIC CHITOSAN; PERMEABLE REACTIVE BARRIERS; REVERSE-OSMOSIS MEMBRANES; AGRICULTURAL BY-PRODUCTS; MORINGA-OLEIFERA SEEDS; CROSS-LINKED CHITOSAN; TEXTILE WASTE-WATER AB Coal mine water (CMW) is typically treated to remove suspended solids, acidity, and soluble metals, but high concentrations of total dissolved solids (TDS) have been reported to impact the environment at several CMW discharge points. Consequently, various states have established TDS wastewater regulations and the US EPA has proposed a benchmark conductivity limit to reduce TDS impacts in streams near mining sites. Traditional CMW treatment effectively removes some TDS components, but is not effective in removing major salt ions due to their higher solubility. This paper describes the basic principles, effectiveness, advantages, and disadvantages of various TDS removal technologies (adsorption, bioremediation, capacitive deionization, desalination, distillation, electrochemical ion exchange, electrocoagulation, electrodialysis, ion exchange, membrane filtration, precipitation, and reverse osmosis) that have at least been tested in bench- and pilot-scale experiments. C1 [Pinto, Patricio X.; Balz, David A.; Smith, Samantha J.] Pegasus Tech Serv Inc, 46 E Hollister St, Cincinnati, OH 45219 USA. [Al-Abed, Souhail R.; Butler, Barbara A.] US EPA, Natl Risk Management Res Lab, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Landy, Ronald B.] US EPA, Off Res & Dev, Ctr Environm Sci, 701 Mapes Rd, Ft George G Meade, MD 20755 USA. RP Al-Abed, SR (reprint author), US EPA, Natl Risk Management Res Lab, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM al-abed.souhail@epa.gov OI Pinto, Patricio/0000-0002-7840-457X FU US EPA National Risk Management Research Laboratory, Cincinnati, Ohio FX This research was funded and performed by the US EPA National Risk Management Research Laboratory, Cincinnati, Ohio. This paper has not been subjected to the Agency's internal review; therefore, the opinions and results presented herein do not necessarily reflect the views of the Agency or its policy. Mention of trade names and commercial products does not constitute endorsement or recommendation for use. The authors thank Dr. Raghuraman Venkatapathy for his contribution to this manuscript. NR 255 TC 2 Z9 2 U1 27 U2 62 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1025-9112 EI 1616-1068 J9 MINE WATER ENVIRON JI Mine Water Environ. PD MAR PY 2016 VL 35 IS 1 BP 94 EP 112 DI 10.1007/s10230-015-0351-7 PG 19 WC Water Resources SC Water Resources GA DF4HK UT WOS:000371308400011 ER PT J AU Phelan, J Belyazid, S Jones, P Cajka, J Buckley, J Clark, C AF Phelan, Jennifer Belyazid, Salim Jones, Phillip Cajka, Jamie Buckley, John Clark, Christopher TI Assessing the Effects of Climate Change and Air Pollution on Soil Properties and Plant Diversity in Sugar Maple-Beech-Yellow Birch Hardwood Forests in the Northeastern United States: Model Simulations from 1900 to 2100 SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Deposition; Climate; Soil; Understory vegetation; Model; Hubbard Brook; Bear Brook ID ATMOSPHERIC NITROGEN DEPOSITION; GLOBAL ENVIRONMENTAL-CHANGES; BEAR BROOK; CRITICAL LOADS; EXPERIMENTAL ACIDIFICATION; ELEVATED CO2; RED SPRUCE; SULFUR DEPOSITION; ACIDIC DEPOSITION; HUBBARD BROOK AB Historical deposition of nitrogen (N) and sulfur (S) over the Eastern United States has impacted ecosystem structure and function. The potential for recovery of ecosystems is relatively uncertain, with deposition paired with future climate change contributing to this uncertainty. The impacts of N and S deposition and climate change (from 1900 to 2100) on two sugar maple-beech-yellow birch sites in the Northeastern United States were evaluated using the paired biogeochemical-vegetation response model ForSAFE-Veg at two research sites, Hubbard Brook Experimental Forest and Bear Brook Watershed. Deposition was found to be the dominant stressor, causing changes in soil acid-base chemistry, N enrichment, and shifts in understory species composition. Responses of the two sites varied due primarily to differences in buffering capacity and levels of deposition. However, at both sites, recovery of soil and plant community properties to 1900 conditions was approached only when future deposition to 2100 was returned to pre-industrial levels. Policy-based reductions in deposition generally halted further damage to soils and plants and resulted in no or only partial recovery. Increased temperatures and precipitation according to Intergovernmental Panel on Climate Change (IPCC) climate futures stimulated soil and plant response, thereby accelerating changes in plant communities and N enrichment and counteracting the acidifying impacts of N and S deposition on soil acid-base chemistry. C1 [Phelan, Jennifer; Jones, Phillip; Cajka, Jamie; Buckley, John] RTI Int, Raleigh, NC USA. [Belyazid, Salim] Belyazid Consulting & Commun AB, Malmo, Sweden. [Clark, Christopher] US EPA, Washington, DC 20460 USA. RP Phelan, J (reprint author), RTI Int, Raleigh, NC USA. EM jenphelan@rti.org FU U.S. EPA [EP-C-11-036] FX This study was funded by the U.S. EPA (contract EP-C-11-036). NR 100 TC 0 Z9 0 U1 11 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD MAR PY 2016 VL 227 IS 3 AR 84 DI 10.1007/s11270-016-2762-x PG 30 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA DF5XW UT WOS:000371427300005 ER PT J AU Baldauf, RW Isakov, V Deshmukh, P Venkatram, A Yang, B Zhang, KM AF Baldauf, Richard W. Isakov, Vlad Deshmukh, Parikshit Venkatram, Akula Yang, Bo Zhang, K. Max TI Influence of solid noise barriers on near-road and on-road air quality SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Air quality; Emissions; Near-road; Traffic; Noise barriers ID PARTICLE-SIZE DISTRIBUTIONS; WIND-TUNNEL; DISPERSION; CONFIGURATIONS; ENVIRONMENT; POLLUTION; EVOLUTION AB Public health concerns regarding adverse health effects for populations spending significant amounts of time near high traffic roadways has increased substantially in recent years. Roadside features, including solid noise barriers, have been investigated as potential methods that can be implemented in a relatively short time period to reduce air pollution exposures from nearby traffic. A field study was conducted to determine the influence of noise barriers on both on-road and downwind pollutant concentrations near a large highway in Phoenix, Arizona, USA. Concentrations of nitrogen dioxide, carbon monoxide, ultrafine particles, and black carbon were measured using a mobile platform and fixed sites along two limited access stretches of highway that contained a section of noise barrier and a section with no noise barrier at-grade with the surrounding terrain. Results of the study showed that pollutant concentrations behind the roadside barriers were significantly lower relative to those measured in the absence of barriers. The reductions ranged from 50% within 50 m from the barrier to about 30% as far as 300 m from the barrier. Reductions in pollutant concentrations generally began within the first 50 m of the barrier edge; however, concentrations were highly variable due to vehicle activity behind the barrier and along nearby urban arterial roadways. The concentrations on the highway, upwind of the barrier, varied depending on wind direction. Overall, the on-road concentrations in front of the noise barrier were similar to those measured in the absence of the barrier, contradicting previous modeling results that suggested roadside barriers increase pollutant levels on the road. Thus, this study suggests that noise barriers do reduce potential pollutant exposures for populations downwind of the road, and do not likely increase exposures to traffic-related pollutants for vehicle passengers on the highway. Published by Elsevier Ltd. C1 [Baldauf, Richard W.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Baldauf, Richard W.] US EPA, Off Transportat & Air Qual, Natl Vehicle & Fuels Emiss Lab, Ann Arbor, MI USA. [Isakov, Vlad] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Deshmukh, Parikshit] ARCADIS, Durham, NC USA. [Venkatram, Akula] Univ Calif Riverside, Dept Mech Engn, Riverside, CA 92521 USA. [Yang, Bo; Zhang, K. Max] Cornell Univ, Sibley Sch Mech & Aerosp Engn, Ithaca, NY 14853 USA. RP Baldauf, RW (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM Baldauf.Richard@epa.gov FU U.S. Environmental Protection Agency FX This research study was fully funded by the U.S. Environmental Protection Agency, and relied on the collaboration and contribution of several partner organizations in the Phoenix area. We thank Ben Davis and the Maricopa County Air Quality Department for graciously providing storage and maintenance facilities for the air quality monitoring equipment. We also thank Wang Zhang and Vladimir Livshits of the Maricopa Association of Governments for traffic data on I-17 during the sampling period, and historical data on fleet mix characteristics for these sections of I-17 in Phoenix. In addition, we thank Darcy Anderson and Beverly Chenausky of the Arizona Department of Transportation for providing insights on sampling locations and assistance with permitting for access to the sampling locations. We also thank add Brian Eder of the U.S. EPA for providing meteorological forecasts to determine the best location for sampling each day. We finally want to thank Halley Brantley and Gayle Hagler of the U.S. EPA for their assistance in data processing and quality assurance analyses. NR 23 TC 1 Z9 1 U1 13 U2 27 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD MAR PY 2016 VL 129 BP 265 EP 276 DI 10.1016/j.atmosenv.2016.01.025 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DE8KX UT WOS:000370886000030 ER PT J AU Kapoor, V Elk, M Li, X Impellitteri, CA Domingo, JWS AF Kapoor, Vikram Elk, Michael Li, Xuan Impellitteri, Christopher A. Domingo, Jorge W. Santo TI Effects of Cr(III) and Cr(VI) on nitrification inhibition as determined by SOUR, function-specific gene expression and 16S rRNA sequence analysis of wastewater nitrifying enrichments SO CHEMOSPHERE LA English DT Article DE Nitrification; SOUR; RT-qPCR; Chromium; Wastewater ID ACTIVATED-SLUDGE PROCESS; NITROSOMONAS-EUROPAEA; HEXAVALENT CHROMIUM; TRANSCRIPTIONAL RESPONSES; TANNERY WASTES; HEAVY-METALS; BACTERIA; TOXICITY; REMOVAL; PERFORMANCE AB The effect of Cr(III) and Cr(VI) on nitrification was examined with samples from nitrifying enrichment cultures using three different approaches: by measuring substrate (ammonia) specific oxygen uptake rates (SOUR), by using RT-qPCR to quantify the transcripts of functional genes involved in nitrification, and by analysis of 16S rRNA sequences to determine changes in structure and activity of the microbial communities. The nitrifying bioreactor was operated as a continuous reactor with a 24 h hydraulic retention time. The samples were exposed in batch vessels to Cr(III) (10-300 mg/L) and Cr(VI) (1-30 mg/L) for a period of 12 h. There was considerable,decrease in SOUR with increasing dosages for both Cr(Ill) and Cr(VI), however Cr(VI) was more inhibitory than Cr(III). Based on the RT-qPCR data, there was reduction in the transcript levels of amoA and hao for increasing Cr(III) dosage, which corresponded well with the ammonia oxidation activity measured via SOUR. For Cr(VI) exposure, there was comparatively little reduction in amoA expression while hao expression decreased for 1-3 mg/L Cr(VI) and increased at 30 mg/L Cr(VI). While Nitrosomonas spp. were the dominant bacteria in the bioreactor, based on 16S rRNA sequencing, there was a considerable reduction in Nitrosomonas activity upon exposure to 300 mg/ L Cr(III). In contrast, a relatively small reduction in activity was observed at 30 mg/L Cr(VI) loading. Our data that suggest that both Cr(III) and Cr(VI) were inhibitory to nitrification at concentrations near the high end of industrial effluent concentrations. Published by Elsevier Ltd. C1 [Kapoor, Vikram; Li, Xuan] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Kapoor, Vikram; Li, Xuan; Impellitteri, Christopher A.; Domingo, Jorge W. Santo] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. [Elk, Michael] Pegasus Tech Serv Inc, Cincinnati, OH 45268 USA. RP Domingo, JWS (reprint author), US EPA, Off Res & Dev, Cincinnati, OH 45268 USA. EM santodomingo.jorge@epa.gov FU Postdoctoral Research Program at the U.S. Environmental Protection Agency (EPA), Office of Research and Development, Cincinnati, OH FX This research was supported in part by an appointment to the Postdoctoral Research Program at the U.S. Environmental Protection Agency (EPA), Office of Research and Development, Cincinnati, OH, administered by the Oak Ridge Institute for Science and Education through an Interagency agreement between the U.S. Department of Energy and the U.S. Environmental Protection Agency. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. NR 44 TC 3 Z9 3 U1 2 U2 31 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD MAR PY 2016 VL 147 BP 361 EP 367 DI 10.1016/j.chemosphere.2015.12.119 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA DE7SD UT WOS:000370836100046 PM 26774300 ER PT J AU Snyder, MN Freeman, MC Purucker, ST Pringle, CM AF Snyder, Marcia N. Freeman, Mary C. Purucker, S. Thomas Pringle, Catherine M. TI Using occupancy modeling and logistic regression to assess the distribution of shrimp species in lowland streams, Costa Rica: does regional groundwater create favorable habitat? SO FRESHWATER SCIENCE LA English DT Article DE crustacean; detection probability; freshwater; Macrobrachium; river; tropical ID ESTIMATING SITE OCCUPANCY; SELVA BIOLOGICAL STATION; PRESENCE-ABSENCE DATA; FRESH-WATER SHRIMP; POPULATION-DYNAMICS; NEOTROPICAL STREAM; TROPICAL STREAMS; CENTRAL-AMERICA; ABUNDANCE; ASSEMBLAGES AB Freshwater shrimps are an important biotic component of tropical ecosystems. However, they can have a low probability of detection when abundances are low. We sampled 3 of the most common freshwater shrimp species, Macrobrachium olfersii, Macrobrachium carcinus, and Macrobrachium heterochirus, and used occupancy modeling and logistic regression models to improve our limited knowledge of distribution of these cryptic species by investigating both local- and landscape-scale effects at La Selva Biological Station in Costa Rica. Local-scale factors included substrate type and stream size, and landscape scale factors included presence or absence of regional groundwater inputs. Capture rates for 2 of the sampled species (M olfersii and M. carcinus) were sufficient to compare the fit of occupancy models. Occupancy models did not converge for M. heterochirus, but M. heterochirus had high enough occupancy rates that logistic regression could be used to model the relationship between occupancy rates and predictors. The best-supported models for M olfersii and M carcinus included conductivity, discharge, and substrate parameters. Stream size was positively correlated with occupancy rates of all 3 species. High stream conductivity, which reflects the quantity of regional groundwater input into the stream, was positively correlated with M olfersii occupancy rates. Boulder substrates increased occupancy rate of M carcinus and decreased the detection probability of M olfersii. Our models suggest that shrimp distribution is driven by factors that function at local (substrate and discharge) and landscape (conductivity) scales. C1 [Snyder, Marcia N.; Pringle, Catherine M.] Univ Georgia, Odum Sch Ecol, 140 East Green St, Athens, GA 30602 USA. [Freeman, Mary C.] Univ Georgia, US Geol Survey, 150 Univ Court, Athens, GA 30602 USA. [Purucker, S. Thomas] US EPA, Off Res & Dev, 960 Coll Stn Rd, Athens, GA 30605 USA. RP Snyder, MN; Pringle, CM (reprint author), Univ Georgia, Odum Sch Ecol, 140 East Green St, Athens, GA 30602 USA.; Freeman, MC (reprint author), Univ Georgia, US Geol Survey, 150 Univ Court, Athens, GA 30602 USA.; Purucker, ST (reprint author), US EPA, Off Res & Dev, 960 Coll Stn Rd, Athens, GA 30605 USA. EM snydermn@gmail.com; mcfreeman@usgs.gov; purucker.tom@epa.gov; cpringle@uga.edu FU National Science Foundation through the Long-Term Studies Environmental Biology program [DEB 9528434, DEB 0075339, DEB 0545463]; Organization for Tropical Studies FX We are grateful to D. Sellers, A. Gilman, and M. Hidalgo for help with field work and to the Pringle lab for helpful feedback that improved this manuscript. T. Maddox and L. Dean contributed to laboratory analysis. This study was supported by the National Science Foundation through the Long-Term Studies Environmental Biology program (DEB 9528434, DEB 0075339, DEB 0545463) and the Organization for Tropical Studies. The use of trade, product, or firm names does not constitute endorsement by the US Government. NR 50 TC 0 Z9 0 U1 4 U2 13 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2161-9549 EI 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD MAR PY 2016 VL 35 IS 1 BP 80 EP 90 DI 10.1086/684486 PG 11 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DE9XS UT WOS:000370993800007 ER PT J AU Hale, RL Scoggins, M Smucker, NJ Suchy, A AF Hale, Rebecca L. Scoggins, Mateo Smucker, Nathan J. Suchy, Amanda TI Effects of climate on the expression of the urban stream syndrome SO FRESHWATER SCIENCE LA English DT Article DE urban; climate; urban stream syndrome; stormwater; geomorphology; hydrology; water infrastructure; impervious cover; habitat; diversity; ecosystem function ID LARGE WOODY DEBRIS; CATCHMENT LAND-USE; FUNCTIONAL INDICATORS; AQUATIC ECOSYSTEMS; UNITED-STATES; USE GRADIENTS; NITROGEN; URBANIZATION; WATER; RIPARIAN AB Urban streams display many similar symptoms around the world, but awareness is growing that the mechanisms and severity of the symptoms differ among regions. Climate is a key contributor to these differences via: 1) variation in the initial conditions of streams across climate (and their sensitivity to urbanization), 2) interactions between climate and urbanization, and 3) indirect effects of climate on the form of urbanization or management via effects on human behaviors. We discuss the direct and indirect pathways by which climate shapes the structure and function of urban streams and how differences in climate affect how the urban stream syndrome is manifested. For some symptoms, such as stream flashiness, climate can affect the direction and magnitude of response, whereas for others, such as losses of sensitive taxa, climate can affect the magnitude of the response. Mechanisms linking urbanization with ecological degradation, even when responses are similar, can differ among climates. Limited research has been done on the role of climate in stream response to urbanization. Controlled, large-scale studies are needed to address this issue, particularly because climate may covary with types of infrastructure and technological capabilities across the globe. Understanding the linkages between climate and urban stream ecosystems will improve our theoretical understanding of urban streams and our ability to implement tailored and regionally sensitive management strategies. C1 [Hale, Rebecca L.] Univ Utah, Global Change & Sustainabil Ctr, Salt Lake City, UT 84112 USA. [Scoggins, Mateo] City Austin, Watershed Protect Dept, Austin, TX 78704 USA. [Smucker, Nathan J.] US EPA, Atlantic Ecol Div, Narragansett, RI 02882 USA. [Suchy, Amanda] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Hale, Rebecca L.] Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA. RP Hale, RL (reprint author), Univ Utah, Global Change & Sustainabil Ctr, Salt Lake City, UT 84112 USA.; Scoggins, M (reprint author), City Austin, Watershed Protect Dept, Austin, TX 78704 USA.; Smucker, NJ (reprint author), US EPA, Atlantic Ecol Div, Narragansett, RI 02882 USA.; Suchy, A (reprint author), Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA.; Hale, RL (reprint author), Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA. EM halereb3@isu.edu; mateo.scoggins@austintexas.gov; smucker.nathan@epa.gov; asuchy@asu.edu FU National Science Foundation (NSF) [DEB 1427007]; NSF under Experimental Program to Stimulate Competitive Research (EPSCoR) [IIA 1208732]; US Environmental Protection Agency (EPA) [ORD 011311]; US Department of Energy [ORD 011311] FX We thank 2 anonymous referees and Associate Editor Allison Roy for their helpful comments, which greatly improved this manuscript. This work resulted from the 3rd Symposium on Urbanization and Stream Ecology, which was supported by National Science Foundation (NSF) DEB 1427007. This work was supported by the NSF under Experimental Program to Stimulate Competitive Research (EPSCoR) grant IIA 1208732 awarded to Utah State University as part of the State of Utah EPSCoR Research Infrastructure Improvement Award. NJS's work (ORD 011311) was supported in part by an appointment to the Oak Ridge Institute for Science and Education (ORISE) participant research program supported by an interagency agreement between the US Environmental Protection Agency (EPA) and the US Department of Energy, but views may not reflect those of the EPA, and no official endorsement should be inferred. NR 75 TC 12 Z9 12 U1 12 U2 31 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2161-9549 EI 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD MAR PY 2016 VL 35 IS 1 BP 421 EP 428 DI 10.1086/684594 PG 8 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DE9XS UT WOS:000370993800033 ER PT J AU Parr, TB Smucker, NJ Bentsen, CN Neale, MW AF Parr, Thomas B. Smucker, Nathan J. Bentsen, Catherine N. Neale, Martin W. TI Potential roles of past, present, and future urbanization characteristics in producing varied stream responses SO FRESHWATER SCIENCE LA English DT Article DE stream ecosystems; urbanization; urban stream syndrome; water infrastructure; impervious cover; urban watershed continuum; stormwater; green infrastructure; water policy; wastewater; resilience; sustainability ID URBAN STORMWATER RUNOFF; DRAINAGE INFRASTRUCTURE; UNITED-STATES; WATER; MANAGEMENT; LANDSCAPE; IMPACT; ECOSYSTEMS; POLLUTION; CONCRETE AB Stormwater drainage and wastewater disposal are primary pathways through which urbanization degrades streams. These technologies and management practices change over time, reshaping the urban template and affecting the environmental challenges a city will face in the following decades to centuries. Spatial and temporal asynchrony in the implementation and replacement of these technologies and the adoption of new management approaches means that the mechanisms of the urban stream syndrome will be heterogeneous. Thus, promoting 'one size fits all' global panaceas for urban streams may be less effective than local solutions based on the unique urban templates and socioeconomic factors governing streams. Understanding the cumulative effects of spatiotemporal changes in urban templates on streams is critical to protecting and managing them because: 1) existing water infrastructure in many countries (especially high-income countries) is aging and requires replacement, 2) urbanization in rapidly developing countries requires ever more infrastructure, and 3) demand for higher levels of environmental quality is transforming technological and management approaches globally. The management and technological decisions made during the current infrastructure replacement or new construction cycle will define the future urban template and select the trajectory and character of the urban stream syndrome during the coming decades. C1 [Parr, Thomas B.] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. [Smucker, Nathan J.] US EPA, Atlantic Ecol Div, Narragansett, RI 02882 USA. [Bentsen, Catherine N.] Univ Massachusetts, Massachusetts Cooperat Fish & Wildlife Res Unit, Amherst, MA 01003 USA. [Neale, Martin W.] Golder Associates, Auckland, New Zealand. [Neale, Martin W.] Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand. RP Parr, TB (reprint author), Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA.; Smucker, NJ (reprint author), US EPA, Atlantic Ecol Div, Narragansett, RI 02882 USA.; Bentsen, CN (reprint author), Univ Massachusetts, Massachusetts Cooperat Fish & Wildlife Res Unit, Amherst, MA 01003 USA.; Neale, MW (reprint author), Golder Associates, Auckland, New Zealand.; Neale, MW (reprint author), Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand. EM tbparr@udel.edu; smucker.nathan@epa.gov; cbentsen@umass.edu; mneale@golder.co.nz FU National Science Foundation [DEB 1427007]; US Environmental Protection Agency (EPA) [ORD-011397]; Department of Energy [ORD-011397] FX We acknowledge National Science Foundation grant DEB 1427007 for funding the 3rd Symposium on Urbanization and Stream Ecology. NJS's work (ORD-011397) was supported in part by an appointment to the Oak Ridge Institute for Science and Education participant research program supported by an interagency agreement between the US Environmental Protection Agency (EPA) and the Department of Energy. Views may not necessarily reflect those of EPA, and no official endorsement should be inferred. NR 58 TC 9 Z9 9 U1 8 U2 31 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2161-9549 EI 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD MAR PY 2016 VL 35 IS 1 BP 436 EP 443 DI 10.1086/685030 PG 8 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DE9XS UT WOS:000370993800035 ER PT J AU Silva, MJ Hilton, D Furr, J Gray, LE Preau, JL Calafat, AM Ye, XY AF Silva, Manori J. Hilton, Donald Furr, Johnathan Gray, L. Earl Preau, James L. Calafat, Antonia M. Ye, Xiaoyun TI Quantification of tetrabromo benzoic acid and tetrabromo phthalic acid in rats exposed to the flame retardant Uniplex FPR-45 SO ARCHIVES OF TOXICOLOGY LA English DT Article DE Flame retardant; EH-TBB; BEH-TEBP; Bis-(2-ethylhexyl)-2,3,4,5-tetrabromophthalate; 2-Ethylhexyl-2,3,4,5-tetrabromobenzoate; Tetrabromo benzoic acid; Tetrabromo phthalic acid ID FIREMASTER(R) 550; INDOOR DUST; METABOLITES; URINARY AB The first withdrawal of certain polybrominated diphenyl ethers flame retardants from the US market occurred in 2004. Since then, use of brominated non-PBDE compounds such as bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate (BEH-TEBP) and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB) in commercial formulations has increased. Assessing human exposure to these chemicals requires identifying metabolites that can potentially serve as their biomarkers of exposure. We administered by gavage a dose of 500 mg/Kg bw of Uniplex FRP-45 (> 95 % BEH-TEBP) to nine adult female Sprague-Dawley rats. Using authentic standards and mass spectrometry, we positively identified and quantified 2,3,4,5-tetrabromo benzoic acid (TBBA) and 2,3,4,5-tetrabromo phthalic acid (TBPA) in 24-h urine samples collected 1 day after dosing the rats and in serum at necropsy, 2 days post-exposure. Interestingly, TBBA and TBPA concentrations correlated well (R (2) = 0.92). The levels of TBBA, a known metabolite of EH-TBB, were much higher than the levels of TBPA both in urine and serum. Because Uniplex FRP-45 was technical grade and EH-TBB was present in the formulation, TBBA likely resulted from the metabolism of EH-TBB. Taken together, our data suggest that TBBA and TBPA may serve as biomarkers of exposure to non-PBDE brominated flame retardant mixtures. Additional research can provide useful information to better understand the composition and in vivo toxicokinetics of these commercial mixtures. C1 [Silva, Manori J.; Hilton, Donald; Preau, James L.; Calafat, Antonia M.; Ye, Xiaoyun] Ctr Dis Control & Prevent, Div Sci Lab, Natl Ctr Environm Hlth, Atlanta, GA 30341 USA. [Furr, Johnathan; Gray, L. Earl] US EPA, Reprod Toxicol Branch, Tox Assessment Div, Natl Hlth & Environm Effects Res Lab,Off Res & De, Res Triangle Pk, NC 27709 USA. RP Silva, MJ (reprint author), Ctr Dis Control & Prevent, Div Sci Lab, Natl Ctr Environm Hlth, Atlanta, GA 30341 USA. EM zca2@cdc.gov FU Intramural CDC HHS [CC999999] NR 17 TC 4 Z9 4 U1 6 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0340-5761 EI 1432-0738 J9 ARCH TOXICOL JI Arch. Toxicol. PD MAR PY 2016 VL 90 IS 3 BP 551 EP 557 DI 10.1007/s00204-015-1489-6 PG 7 WC Toxicology SC Toxicology GA DE0VJ UT WOS:000370343000005 PM 25804200 ER PT J AU Creutzburg, MK Scheller, RM Lucash, MS Evers, LB Leduc, SD Johnson, MG AF Creutzburg, Megan K. Scheller, Robert M. Lucash, Melissa S. Evers, Louisa B. Leduc, Stephen D. Johnson, Mark G. TI Bioenergy harvest, climate change, and forest carbon in the Oregon Coast Range SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE bioenergy; biomass energy; carbon; climate change; forest; LANDIS-II; landscape modeling; Oregon Coast Range; simulation modeling ID NET PRIMARY PRODUCTION; DOUGLAS-FIR; PACIFIC-NORTHWEST; WESTERN OREGON; UNITED-STATES; TEMPERATE FORESTS; SIMULATION-MODEL; BRITISH-COLUMBIA; HEMLOCK FORESTS; USE EFFICIENCY AB Forests provide important ecological, economic, and social services, and recent interest has emerged in the potential for using residue from timber harvest as a source of renewable woody bioenergy. The long-term consequences of such intensive harvest are unclear, particularly as forests face novel climatic conditions over the next century. We used a simulation model to project the long-term effects of management and climate change on above- and belowground forest carbon storage in a watershed in northwestern Oregon. The multi-ownership watershed has a diverse range of current management practices, including little-to-no harvesting on federal lands, short-rotation clear-cutting on industrial land, and a mix of practices on private nonindustrial land. We simulated multiple management scenarios, varying the rate and intensity of harvest, combined with projections of climate change. Our simulations project a wide range of total ecosystem carbon storage with varying harvest rate, ranging from a 45% increase to a 16% decrease in carbon compared to current levels. Increasing the intensity of harvest for bioenergy caused a 2-3% decrease in ecosystem carbon relative to conventional harvest practices. Soil carbon was relatively insensitive to harvest rotation and intensity, and accumulated slowly regardless of harvest regime. Climate change reduced carbon accumulation in soil and detrital pools due to increasing heterotrophic respiration, and had small but variable effects on aboveground live carbon and total ecosystem carbon. Overall, we conclude that current levels of ecosystem carbon storage are maintained in part due to substantial portions of the landscape (federal and some private lands) remaining unharvested or lightly managed.Increasing the intensity of harvest for bioenergy on currently harvested land, however,led to a relatively small reduction in the ability of forests to store carbon. Climate change is unlikely to substantially alter carbon storage in these forests, absent shifts in disturbance regimes. C1 [Creutzburg, Megan K.; Scheller, Robert M.; Lucash, Melissa S.] Portland State Univ, Dept Environm Sci & Management, POB 751, Portland, OR 97207 USA. [Evers, Louisa B.] Bur Land Management, 1220 SW 3rd Ave, Portland, OR 97204 USA. [Leduc, Stephen D.] US EPA, Natl Ctr Environm Assessment, 1200 Penn Ave,NW 8623P, Washington, DC 20460 USA. [Johnson, Mark G.] US EPA, Natl Hlth & Environm Effects Res Lab, 200 SW 35th St, Corvallis, OR 97333 USA. RP Creutzburg, MK (reprint author), Portland State Univ, Dept Environm Sci & Management, POB 751, Portland, OR 97207 USA. EM mkc3@pdx.edu RI Scheller, Robert/B-3135-2009 FU U.S. Bureau of Land Management and Environmental Protection Agency [DW-14-95829801-0] FX Funding was provided by the U.S. Bureau of Land Management and Environmental Protection Agency Interagency Agreement DW-14-95829801-0. Thanks to George McFadden of the Bureau of Land Management, and Greg Johnson, David Marshall, and Scott Holub of Weyerhaeuser for project input. Thanks to the Landscape Ecology, Modeling, Mapping and Analysis team for providing vegetation maps and the Ameriflux network for providing flux tower data. Special thanks to the members of the Dynamic Ecosystem and Landscapes Lab at Portland State University for help and feedback throughout this study, with particular thanks to Alec Kretchun and Matthew Duveneck for their willingness to share their expertise in LANDIS-II modeling. Two anonymous reviewers provided comments that improved an earlier version of this manuscript. The views expressed here are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency or Bureau of Land Management. NR 88 TC 2 Z9 2 U1 4 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD MAR PY 2016 VL 8 IS 2 BP 357 EP 370 DI 10.1111/gcbb.12255 PG 14 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DE2XU UT WOS:000370492100009 ER PT J AU Canu, IG Bateson, TF Bouvard, V Debia, M Dion, C Savolainen, K Yu, IJ AF Canu, Irina Guseva Bateson, Thomas F. Bouvard, Veronique Debia, Maximilien Dion, Chantal Savolainen, Kai Yu, Ii-Je TI Human exposure to carbon-based fibrous nanomaterials: A review SO INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH LA English DT Review DE Carbon nanotubes; Carbon nanofibers; Life-cycle; Exposure metric ID EPOXY-BASED NANOCOMPOSITE; ASSESSMENT TECHNIQUE NEAT; ENGINEERED NANOMATERIALS; OCCUPATIONAL-EXPOSURE; SECONDARY MANUFACTURERS; ENVIRONMENTAL EXPOSURE; NANOPARTICLE RELEASE; AIRBORNE PARTICLES; NANOFIBER PRIMARY; NANO-OBJECTS AB In an emerging field of nanotechnologies, assessment of exposure to carbon nanotubes (CNT) and carbon nanofibers (CNF) is an integral component of occupational and environmental epidemiology, risk assessment and management, as well as regulatory actions. The current state of knowledge on exposure to carbon-based fibrous nanomaterials among workers, consumers and general population was studied in frame of the International Agency for Research on Cancer (IARC) Monographs-Volume 111 "Some Nanomaterials and Some Fibres". Completeness and reliability of available exposure data for use in epidemiology and risk assessment were assessed. Occupational exposure to CNT/CNF may be of concern at all stages of the material life-cycle from research through manufacture to use and disposal. Consumer and environmental exposures are only estimated by modeled data. The available information of the final steps of the life-cycle of these materials remains incomplete so far regarding amounts of handled materials and levels of exposure. The quality and amount of information available on the uses and applications of CNT/CNF should be improved to enable quantitative assessment of human exposure to these materials. For that, coordinated effort in producing surveys and exposure inventories based on harmonized strategy of material test, exposure measurement and reporting results is strongly encouraged. (C) 2015 Elsevier GmbH. All rights reserved. C1 [Canu, Irina Guseva] Inst Veille Sanit, Dept Sante Travail, 12 Rue Val Osne, F-94415 St Maurice, France. [Bateson, Thomas F.] US EPA, Effects Identificat & Characterizat Grp, Washington, DC 20460 USA. [Bouvard, Veronique] IARC, IARC Monographs Sect, Lyon, France. [Debia, Maximilien; Dion, Chantal] Univ Montreal, Inst Rech Sante Publ, Dept Sante Environm & Sante Travail, Montreal, PQ, Canada. [Savolainen, Kai] Finnish Inst Occupat Hlth, Nanosafety Res Ctr, Helsinki, Finland. [Yu, Ii-Je] Hoseo Univ, Toxicol Res Ctr, Asan, South Korea. RP Canu, IG (reprint author), Inst Veille Sanit, Dept Sante Travail, 12 Rue Val Osne, F-94415 St Maurice, France. EM i.guseva-canu@invs.sante.fr NR 68 TC 3 Z9 3 U1 7 U2 44 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1438-4639 EI 1618-131X J9 INT J HYG ENVIR HEAL JI Int. J. Hyg. Environ. Health. PD MAR PY 2016 VL 219 IS 2 BP 166 EP 175 DI 10.1016/j.ijheh.2015.12.005 PG 10 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA DE6VG UT WOS:000370771100002 ER PT J AU Silvestri, EE Perkins, SD Rice, EW Stone, H Schaefer, FW AF Silvestri, Erin E. Perkins, Sarah D. Rice, Eugene W. Stone, Harry Schaefer, Frank W., III TI Review of processing and analytical methods for Francisella tularensis in soil and water SO ANNALS OF MICROBIOLOGY LA English DT Review DE Francisella tularensis; Soil; Drinking water; Surface water; Processing; Analysis ID REAL-TIME PCR; LINKED-IMMUNOSORBENT-ASSAY; POLYMERASE-CHAIN-REACTION; BACILLUS-ANTHRACIS; ENVIRONMENTAL-SAMPLES; RAPID DETECTION; YERSINIA-PESTIS; MOLECULAR EPIDEMIOLOGY; BIOTHREAT AGENTS; FIELD DETECTION AB The etiological agent of tularemia, Francisella tularensis, is a resilient organism within the environment and can be acquired in many ways (infectious aerosols and dust, contaminated food and water, infected carcasses, and arthropod bites). However, isolating F. tularensis from environmental samples can be challenging due to its nutritionally fastidious and slow-growing nature. In order to determine the current state of the science regarding available processing and analytical methods for detection and recovery of F. tularensis from water and soil matrices, a review of the literature was conducted. During the review, analysis via culture, immunoassays, and genomic identification were the methods most commonly found for F. tularensis detection within environmental samples. Other methods included combined culture and genomic analysis for rapid quantification of viable microorganisms and use of one assay to identify multiple pathogens from a single sample. Gaps in the literature that were identified during this review suggest that further work to integrate culture and genomic identification would advance our ability to detect and to assess the viability of Francisella spp. The optimization of DNA extraction, whole genome amplification with inhibition-resistant polymerases, and multiagent microarray detection would also advance biothreat detection. C1 [Silvestri, Erin E.; Rice, Eugene W.; Schaefer, Frank W., III] US EPA, Natl Homeland Secur Res Ctr, 26 W Martin Luther King Dr,MS NG16, Cincinnati, OH 45268 USA. [Perkins, Sarah D.; Stone, Harry] Battelle Mem Inst, 505 King Ave, Columbus, OH 43201 USA. RP Silvestri, EE (reprint author), US EPA, Natl Homeland Secur Res Ctr, 26 W Martin Luther King Dr,MS NG16, Cincinnati, OH 45268 USA. EM Silvestri.Erin@epa.gov FU US Environmental Protection Agency, through its Office of Research and Development [SP0700-00-D-3180, 0729, CB-11-0232] FX The US Environmental Protection Agency, through its Office of Research and Development, funded and managed the literature review described herein under an Interagency Agreement with the Defense Technical Information Center through the Battelle/Chemical, Biological, Radiological, and Nuclear Defense Information and Analysis Center Contract No. SP0700-00-D-3180 Delivery Order 0729/Technical Area Task CB-11-0232. This document has been subjected to the Agency's review and has been approved for publication. This report was generated using references (secondary data) that could not be evaluated for accuracy, precision, representativeness, completeness, or comparability and therefore no assurance can be made that the data extracted from these publications meet EPA's stringent quality assurance requirement. The contents of this document reflect the views of the contributors and do not necessarily reflect the views of the Agency. Mention of trade names or commercial products in this document or in the literature referenced in this document does not constitute endorsement or recommendation for use. NR 92 TC 1 Z9 1 U1 7 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1590-4261 EI 1869-2044 J9 ANN MICROBIOL JI Ann. Microbiol. PD MAR PY 2016 VL 66 IS 1 BP 77 EP 89 DI 10.1007/s13213-015-1144-8 PG 13 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DD7BM UT WOS:000370078700006 ER PT J AU Smucker, NJ Kuhn, A Charpentier, MA Cruz-Quinones, CJ Elonen, CM Whorley, SB Jicha, TM Serbst, JR Hill, BH Wehr, JD AF Smucker, Nathan J. Kuhn, Anne Charpentier, Michael A. Cruz-Quinones, Carlos J. Elonen, Colleen M. Whorley, Sarah B. Jicha, Terri M. Serbst, Jonathan R. Hill, Brian H. Wehr, John D. TI Quantifying Urban Watershed Stressor Gradients and Evaluating How Different Land Cover Datasets Affect Stream Management SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Impervious cover; Nutrients; Watershed management; Nitrogen; Coastal watersheds; National land cover database (NLCD) ID CONTERMINOUS UNITED-STATES; NUTRIENT CRITERIA; CATCHMENT URBANIZATION; GREEN INFRASTRUCTURE; IMPERVIOUS SURFACES; ACCURACY ASSESSMENT; NATURAL-RESOURCES; SAMPLING DESIGNS; NITROGEN; RIVERS AB Watershed management and policies affecting downstream ecosystems benefit from identifying relationships between land cover and water quality. However, different data sources can create dissimilarities in land cover estimates and models that characterize ecosystem responses. We used a spatially balanced stream study (1) to effectively sample development and urban stressor gradients while representing the extent of a large coastal watershed (> 4400 km(2)), (2) to document differences between estimates of watershed land cover using 30-m resolution national land cover database (NLCD) and < 1-m resolution land cover data, and (3) to determine if predictive models and relationships between water quality and land cover differed when using these two land cover datasets. Increased concentrations of nutrients, anions, and cations had similarly significant correlations with increased watershed percent impervious cover (IC), regardless of data resolution. The NLCD underestimated percent forest for 71/76 sites by a mean of 11 % and overestimated percent wetlands for 71/76 sites by a mean of 8 %. The NLCD almost always underestimated IC at low development intensities and overestimated IC at high development intensities. As a result of underestimated IC, regression models using NLCD data predicted mean background concentrations of NO3 (-) and Cl- that were 475 and 177 %, respectively, of those predicted when using finer resolution land cover data. Our sampling design could help states and other agencies seeking to create monitoring programs and indicators responsive to anthropogenic impacts. Differences between land cover datasets could affect resource protection due to misguided management targets, watershed development and conservation practices, or water quality criteria. C1 [Smucker, Nathan J.] US EPA, Atlant Ecol Div, Narragansett, RI USA. [Kuhn, Anne; Serbst, Jonathan R.] US EPA, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Atlantic Ecol Div, Narragansett, RI USA. [Charpentier, Michael A.] Raytheon Co, Narragansett, RI USA. [Cruz-Quinones, Carlos J.] Univ Puerto Rico, US EPA, Greater Res Opportun Undergrad Program, San Juan, PR 00936 USA. [Elonen, Colleen M.; Jicha, Terri M.; Hill, Brian H.] US EPA, Natl Hlth & Environm Effects Res Lab, Midcontinent Ecol Div, Off Res & Dev, Duluth, MN USA. [Whorley, Sarah B.; Wehr, John D.] Fordham Univ, Louis Calder Ctr Biol Field Stn, Armonk, NY USA. [Whorley, Sarah B.; Wehr, John D.] Fordham Univ, Dept Biol Sci, Armonk, NY USA. RP Smucker, NJ (reprint author), US EPA, Atlant Ecol Div, Narragansett, RI USA. EM smucker.nathan@epa.gov FU United States Environmental Protection Agency (EPA); Department of Energy; EPA FX Emily Seelen and Joe Bishop assisted with field sampling and laboratory processing of samples. Comments on an earlier draft by Jeff Hollister, Taylor Jarnagin, Betty Kreakie, James Lake, Anthony Olsen, and Glen Thursby are greatly appreciated. NJS research (1) was supported in part by an appointment to the Oak Ridge Institute for Science and Education participant research program supported by an interagency agreement between the United States Environmental Protection Agency (EPA) and Department of Energy and (2) may not necessarily reflect the views of EPA, and no official endorsement should be inferred. Research described in this article has been funded by the EPA, and this manuscript, tracking number ORD-10047, has been reviewed by the Atlantic Ecology Division and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 83 TC 0 Z9 0 U1 6 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD MAR PY 2016 VL 57 IS 3 BP 683 EP 695 DI 10.1007/s00267-015-0629-3 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DD8SY UT WOS:000370198400014 PM 26614349 ER PT J AU Chaffin, BC Garmestani, AS Gosnell, H Craig, RK AF Chaffin, B. C. Garmestani, A. S. Gosnell, H. Craig, R. K. TI Institutional networks and adaptive water governance in the Klamath River Basin, USA SO ENVIRONMENTAL SCIENCE & POLICY LA English DT Article DE Adaptive governance; Social network analysis (SNA); Klamath River Basin; Water governance; Institutional analysis ID NATURAL-RESOURCE MANAGEMENT; SOCIAL-ECOLOGICAL SYSTEMS; ECOSYSTEM MANAGEMENT; CLIMATE-CHANGE; COMANAGEMENT; RESILIENCE; SWEDEN; TIES AB Polycentric networks of formal organizations and informal stakeholder groups, as opposed to centralized institutional hierarchies, can be critically important for strengthening the capacity of governance systems to adapt to unexpected social and biophysical change. Adaptive governance is one type of environmental governance characterized by the emergence of networks that stimulate adaptive capacity through increases in social-learning, communication, trust, public participation and adaptive management. However, detecting and analyzing adaptive governance networks remains elusive, especially given contexts of highly contested resource governance such as large-scale negotiations over water use. Research methods such as social network analysis (SNA) are often infeasible as they necessitate collecting in-depth and politically sensitive personal data from a near-complete set of actors or organizations in a network. Here we present a method for resolving this problem by describing the results of an institutional SNA aimed at characterizing the changing governance network in the Klamath River Basin, USA during a period of contested negotiations over water. Through this research, we forward a method of institutional SNA useful when an individual or egocentric approach to SNA is problematic for political, logistical or financial reasons. We focus our analysis on publically available data signaling changes in formal relationships (statutory, regulatory, contractual) between organizations and stakeholder groups. We find that employing this type of SNA is useful for describing potential and actual transitions in governance that yield increases in adaptive capacity to respond to social and biophysical surprises such as increasing water scarcity and changes in water distribution. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Chaffin, B. C.] Univ Montana, Coll Forestry & Conservat, 32 Campus Dr, Missoula, MT 59812 USA. [Garmestani, A. S.] US EPA, Natl Risk Management Res Lab, 26 West Martin Luther King Jr Dr, Cincinnati, OH 45268 USA. [Gosnell, H.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, 104 CEOAS Adm Bldg, Corvallis, OR 97331 USA. [Craig, R. K.] Univ Utah, SJ Quinney Coll Law, 380 South Univ St, Salt Lake City, UT 84112 USA. RP Chaffin, BC (reprint author), Univ Montana, Coll Forestry & Conservat, 32 Campus Dr, Missoula, MT 59812 USA. EM brian.chaffin@umontana.edu; garmestani.ahjond@epa.gov; gosnellh@geo.oregonstate.edu; robin.craig@law.utah.edu FU U.S. Environmental Protection Agency (EPA) STAR Graduate Fellowship [917277]; Association of California Water Agencies; National Research Council Research Associateship Award FX This research was partially supported by a U.S. Environmental Protection Agency (EPA) STAR Graduate Fellowship (#917277); scholarship funding from the Association of California Water Agencies; and a National Research Council Research Associateship Award administered at the National Risk Management Research Laboratory, U.S. EPA, Cincinnati, OH. NR 52 TC 3 Z9 3 U1 7 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1462-9011 EI 1873-6416 J9 ENVIRON SCI POLICY JI Environ. Sci. Policy PD MAR PY 2016 VL 57 BP 112 EP 121 DI 10.1016/j.envsci.2015.11.008 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DD7GJ UT WOS:000370091400013 ER PT J AU Ganju, NK Brush, MJ Rashleigh, B Aretxabaleta, AL del Barrio, P Grear, JS Harris, LA Lake, SJ McCardell, G O'Donnell, J Ralston, DK Signell, RP Testa, JM Vaudrey, JMP AF Ganju, Neil K. Brush, Mark J. Rashleigh, Brenda Aretxabaleta, Alfredo L. del Barrio, Pilar Grear, Jason S. Harris, Lora A. Lake, Samuel J. McCardell, Grant O'Donnell, James Ralston, David K. Signell, Richard P. Testa, Jeremy M. Vaudrey, Jamie M. P. TI Progress and Challenges in Coupled Hydrodynamic-Ecological Estuarine Modeling SO ESTUARIES AND COASTS LA English DT Review DE Numerical modeling; Hydrodynamics; Ecological modeling; Ecosystem modeling; Skill assessment ID WATER-QUALITY MODELS; GULF-OF-MEXICO; GOVERNING PHYTOPLANKTON BLOOMS; PHYSICAL-BIOLOGICAL MODEL; MARINE ECOSYSTEM MODELS; PARTICLE TRACKING MODEL; PARTIALLY MIXED ESTUARY; NEUSE RIVER ESTUARY; CHESAPEAKE BAY; SKILL ASSESSMENT AB Numerical modeling has emerged over the last several decades as a widely accepted tool for investigations in environmental sciences. In estuarine research, hydrodynamic and ecological models have moved along parallel tracks with regard to complexity, refinement, computational power, and incorporation of uncertainty. Coupled hydrodynamic-ecological models have been used to assess ecosystem processes and interactions, simulate future scenarios, and evaluate remedial actions in response to eutrophication, habitat loss, and freshwater diversion. The need to couple hydrodynamic and ecological models to address research and management questions is clear because dynamic feedbacks between biotic and physical processes are critical interactions within ecosystems. In this review, we present historical and modern perspectives on estuarine hydrodynamic and ecological modeling, consider model limitations, and address aspects of model linkage, skill assessment, and complexity. We discuss the balance between spatial and temporal resolution and present examples using different spatiotemporal scales. Finally, we recommend future lines of inquiry, approaches to balance complexity and uncertainty, and model transparency and utility. It is idealistic to think we can pursue a "theory of everything" for estuarine models, but recent advances suggest that models for both scientific investigations and management applications will continue to improve in terms of realism, precision, and accuracy. C1 [Ganju, Neil K.; Aretxabaleta, Alfredo L.; Signell, Richard P.] US Geol Survey, Woods Hole, MA 02543 USA. [Brush, Mark J.; Lake, Samuel J.] Virginia Inst Marine Sci, Gloucester Point, VA 23062 USA. [Rashleigh, Brenda; Grear, Jason S.] US EPA, Narragansett, RI USA. [del Barrio, Pilar] IH Cantabria, Santander, Spain. [Harris, Lora A.; Testa, Jeremy M.] Univ Maryland, Chesapeake Biol Lab, Solomons, MD 20688 USA. [McCardell, Grant; O'Donnell, James; Vaudrey, Jamie M. P.] Univ Connecticut, Groton, CT USA. [Ralston, David K.] Woods Hole Oceanog Inst, Woods Hole, MA 02543 USA. RP Ganju, NK (reprint author), US Geol Survey, Woods Hole, MA 02543 USA. EM nganju@usgs.gov OI Aretxabaleta, Alfredo/0000-0002-9914-8018; Signell, Richard/0000-0003-0682-9613; Ganju, Neil/0000-0002-1096-0465 FU USGS Coastal and Marine Geology Program; NSF [OCE-1314642]; NIEHS [1P50-ES021923-01]; NOAA NOS NCCOS [NA05NOS4781201, NA11NOS4780043]; Strategic Environmental Research and Development Program-Defense Coastal/Estuarine Research Program [RC-1413, RC-2245]; U.S. Environmental Protection Agency FX This review was developed during a modeling workshop convened at the U.S. Geological Survey Woods Hole Coastal and Marine Science Center, in September 2013. We thank Lisa Lucas and two anonymous reviewers for their input. NKG, ALA, and RPS acknowledge support from the USGS Coastal and Marine Geology Program. DKR gratefully acknowledges support from NSF (OCE-1314642) and NIEHS (1P50-ES021923-01). MJB and JMPV gratefully acknowledge support from NOAA NOS NCCOS (NA05NOS4781201 and NA11NOS4780043). MJB and SJL gratefully acknowledge support from the Strategic Environmental Research and Development Program-Defense Coastal/Estuarine Research Program (RC-1413 and RC-2245). Views, opinions, and/or findings contained in this report are those of the authors and should not be construed as an official U.S. Department of Defense position or decision unless so designated by other official documentation. Although the research described in this article has been funded in part by the U.S. Environmental Protection Agency, it has not been subjected to Agency review. Therefore, it does not necessarily reflect the views of the Agency. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 218 TC 1 Z9 1 U1 12 U2 38 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1559-2723 EI 1559-2731 J9 ESTUAR COAST JI Estuaries Coasts PD MAR PY 2016 VL 39 IS 2 BP 311 EP 332 DI 10.1007/s12237-015-0011-y PG 22 WC Environmental Sciences; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DC9GA UT WOS:000369527900002 PM 27721675 ER PT J AU Frischknecht, R Fantke, P Tschumperlin, L Niero, M Anton, A Bare, J Boulay, AM Cherubini, F Hauschild, MZ Henderson, A Levasseur, A McKone, TE Michelsen, O Canals, LMI Pfister, S Ridoutt, B Rosenbaum, RK Verones, F Vigon, B Jolliet, O AF Frischknecht, Rolf Fantke, Peter Tschuemperlin, Laura Niero, Monia Anton, Assumpcio Bare, Jane Boulay, Anne-Marie Cherubini, Francesco Hauschild, Michael Z. Henderson, Andrew Levasseur, Annie McKone, Thomas E. Michelsen, Ottar Canals, Llorenc Mila i Pfister, Stephan Ridoutt, Brad Rosenbaum, Ralph K. Verones, Francesca Vigon, Bruce Jolliet, Olivier TI Global guidance on environmental life cycle impact assessment indicators: progress and case study SO INTERNATIONAL JOURNAL OF LIFE CYCLE ASSESSMENT LA English DT Article DE Footprint; Guidance; Impact indicators; LCIA; Life cycle impact assessment; Rice cultivation ID LAND-USE IMPACTS; RICE PRODUCTION; BIODIVERSITY; FOOTPRINT; LCA AB The life cycle impact assessment (LCIA) guidance flagship project of the United Nations Environment Programme (UNEP)/Society of Environmental Toxicology and Chemistry (SETAC) Life Cycle Initiative aims at providing global guidance and building scientific consensus on environmental LCIA indicators. This paper presents the progress made since 2013, preliminary results obtained for each impact category and the description of a rice life cycle assessment (LCA) case study designed to test and compare LCIA indicators. The effort has been focused in a first stage on impacts of global warming, fine particulate matter emissions, water use and land use, plus cross-cutting issues and LCA-based footprints. The paper reports the process and progress and specific results obtained in the different task forces (TFs). Additionally, a rice LCA case study common to all TF has been developed. Three distinctly different scenarios of producing and cooking rice have been defined and underlined with life cycle inventory data. These LCAs help testing impact category indicators which are being developed and/or selected in the harmonisation process. The rice LCA case study further helps to ensure the practicality of the finally recommended impact category indicators. The global warming TF concludes that analysts should explore the sensitivity of LCA results to metrics other than GWP. The particulate matter TF attained initial guidance of how to include health effects from PM2.5 exposures consistently into LCIA. The biodiversity impacts of land use TF suggests to consider complementary metrics besides species richness for assessing biodiversity loss. The water use TF is evaluating two stress-based metrics, AWaRe and an alternative indicator by a stakeholder consultation. The cross-cutting issues TF agreed upon maintaining disability-adjusted life years (DALY) as endpoint unit for the safeguard subject "human health". The footprint TF defined main attributes that should characterise all footprint indicators. "Rice cultivation" and "cooking" stages of the rice LCA case study contribute most to the environmental impacts assessed. The results of the TF will be documented in white papers and some published in scientific journals. These white papers represent the input for the Pellston workshop((TM)), taking place in Valencia, Spain, from 24 to 29 January 2016, where best practice, harmonised LCIA indicators and an update on the general LCIA framework will be discussed and agreed on. With the diversity in results and the multi-tier supply chains, the rice LCA case study is well suited to test candidate recommended indicators and to ensure their applicability in common LCA case studies. C1 [Frischknecht, Rolf; Tschuemperlin, Laura] Treeze Ltd, Uster, Switzerland. [Fantke, Peter; Niero, Monia; Hauschild, Michael Z.] Tech Univ Denmark, Dept Engn Management, DK-2800 Lyngby, Denmark. [Bare, Jane; Henderson, Andrew] US EPA, Syst Anal Branch, Sustainable Technol Div, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Boulay, Anne-Marie; Levasseur, Annie] Polytech Montreal, Dept Chem Engn, CIRAIG, Montreal, PQ, Canada. [Cherubini, Francesco] Norwegian Univ Sci & Technol, Dept Energy & Proc Engn, Ind Ecol Programme, N-7034 Trondheim, Norway. [McKone, Thomas E.] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA. [Michelsen, Ottar] Norwegian Univ Sci & Technol, NTNU Sustainabil, NO-7491 Trondheim, Norway. [Canals, Llorenc Mila i] United Nations Environm Programme, Div Technol Ind & Econ, Paris, France. [Pfister, Stephan] ETHZ, Swiss Fed Inst Technol, Zurich, Switzerland. [Ridoutt, Brad] CSIRO, Private Bag 10, Clayton, Vic 3169, Australia. [Rosenbaum, Ralph K.] Irstea, UMR ITAP, ELSA Res Grp, 361 Rue JF Breton,5095, F-34196 Montpellier, France. [Rosenbaum, Ralph K.] ELSA PACT, Ind Chair Environm & Social Sustainabil Assessmen, 361 Rue J Breton,5095, F-34196 Montpellier, France. [Vigon, Bruce] SETAC, Pensacola, FL USA. [Jolliet, Olivier] Univ Michigan, Sch Publ Hlth, Environm Hlth Sci, Ann Arbor, MI 48109 USA. [Ridoutt, Brad] Univ Free State, Dept Agr Econ, ZA-9300 Bloemfontein, South Africa. [Anton, Assumpcio] IRTA, Inst Food & Agr Res & Technol, Barcelona, Spain. RP Frischknecht, R (reprint author), Treeze Ltd, Uster, Switzerland. EM frischknecht@treeze.ch RI Ridoutt, Bradley/D-3329-2011; Niero, Monia/P-4758-2016; OI Ridoutt, Bradley/0000-0001-7352-0427; Niero, Monia/0000-0001-6275-6944; Fantke, Peter/0000-0001-7148-6982; Hauschild, Michael Zwicky/0000-0002-8331-7390; Rosenbaum, Ralph/0000-0002-7620-1568 FU UNEP/SETAC Life Cycle Initiative FX The authors acknowledge the contributions from the participants of the Basel, Barcelona and Adelaide workshops and of the different TFs, as well as the UNEP/SETAC Life Cycle Initiative for funding this activity. NR 35 TC 7 Z9 7 U1 10 U2 40 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0948-3349 EI 1614-7502 J9 INT J LIFE CYCLE ASS JI Int. J. Life Cycle Assess. PD MAR PY 2016 VL 21 IS 3 BP 429 EP 442 DI 10.1007/s11367-015-1025-1 PG 14 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DD6YI UT WOS:000370070200012 ER PT J AU Meserve, PL Vasquez, H Kelt, DA Gutierrez, JR Milstead, WB AF Meserve, Peter L. Vasquez, Hernan Kelt, Douglas A. Gutierrez, Julio R. Milstead, W. Bryan TI Patterns in arthropod abundance and biomass in the semiarid thorn scrub of Bosque Fray Jorge National Park, north-central Chile: A preliminary assessment SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Long-term studies; Climate change; Arthropods; Semiarid systems; Chile ID SMALL MAMMAL ASSEMBLAGE; ECOLOGY AB In 2003, we initiated a sampling protocol for arthropods as part of long-term field experiment in a semiarid thorn scrub community in north-central Chile. We utilized pitfall and Malaise traps to sample terrestrial and volant arthropods, respectively. Inventories were conducted for 4 days and nights monthly. We posited that arthropod abundance and biomass would track environmental changes such as high rainfall often due to El Nino Southern Oscillations (ENSOs). Peaks in both abundance and biomass for terrestrial and volant arthropods were in similar months (spring early summer; August November); arthropods also tracked high rainfall years in 2004, 2006, and 2011, due mostly to increases in a few dominant groups, i.e. tenebrionid beetles (Coleoptera) in terrestrial sampling, and moths (Lepidoptera: Noctuidae, microlepidoptera) in aerial sampling. However, the same groups increased dramatically in 2008 which was a below-average rainfall year. Numerical decreases in late summer-fall months (January-April) were more abrupt for terrestrial arthropods than for volant ones. Both terrestrial and volant arthropods reached their lowest levels in winter months (June-July). Some evidence suggests long-term shifts in the composition of volant taxa. Arthropods likely are important prey items for insectivorous reptiles (i.e., lizards), birds, and an insectivorous small mammal, the elegant mouse opossum (Thylamys elegans), and future efforts are being directed towards examining the evidence for correlated changes in those consumers with arthropod abundance and biomass. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Meserve, Peter L.] No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA. [Vasquez, Hernan; Gutierrez, Julio R.] Univ La Serena, Dept Biol, Casilla 599, La Serena, Chile. [Kelt, Douglas A.] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, Davis, CA 95616 USA. [Gutierrez, Julio R.] Univ La Serena, Casilla 599, La Serena, Chile. [Gutierrez, Julio R.] CEAZA, Casilla 599, La Serena, Chile. [Gutierrez, Julio R.] IEB, Casilla 653, Santiago, Chile. [Milstead, W. Bryan] US EPA, Natl Hlth & Environm Effects Lab, Atlantic Ecol Div, Narragansett, RI 02882 USA. [Meserve, Peter L.] Univ Idaho, Dept Biol Sci, Moscow, ID 83844 USA. RP Meserve, PL (reprint author), No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA.; Meserve, PL (reprint author), Univ Idaho, Dept Biol Sci, Moscow, ID 83844 USA. EM pmeserve@uidaho.edu FU FONDECYT Chile; MIDEPLAN ICM [P05-002]; CONICYT [PFB-23]; U.S. AID Program; Northern Illinois University; University of California, Davis; [DEB-1456729] FX We are grateful to many sources of financial support for this project over 26 years including the U.S. National Science Foundation (most recently DEB-1456729 to DAM and PLM), FONDECYT Chile, MIDEPLAN ICM P05-002, CONICYT PFB-23, the U.S. AID Program, Northern Illinois University, and the University of California, Davis. Many people too numerous to mention have contributed to the project. Steve Heydon (UC Davis Bohart Museum of Entomology) helped us to update the taxonomy of arthropods and provided insight to the natural history of key groups. Finally, we gratefully acknowledge the cooperation of the Corporacion Nacional Forestal (CONAF) in Chile which has allowed us to transform the study area to a living laboratory. NR 26 TC 1 Z9 1 U1 5 U2 18 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD MAR PY 2016 VL 126 SI SI BP 68 EP 75 DI 10.1016/j.jaridenv.2015.11.002 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DD7JT UT WOS:000370101200009 ER PT J AU Meserve, PL Kelt, DA Gutierrez, JR Previtali, MA Milstead, WB AF Meserve, Peter L. Kelt, Douglas A. Gutierrez, Julio R. Andrea Previtali, M. Milstead, W. Bryan TI Biotic interactions and community dynamics in the semiarid thorn scrub of Bosque Fray Jorge National Park, north-central Chile: A paradigm revisited SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Long-term studies; Population; Community ecology; Small mammals; Chile ID AUSTRALIAN DESERT HABITATS; SMALL MAMMAL ASSEMBLAGE; BOTTOM-UP CONTROL; LONG-TERM; POPULATION-DYNAMICS; FIELD EXPERIMENTS; SOUTH-AMERICA; EL-NINO; INTERSPECIFIC COMPETITION; VERTEBRATE PREDATORS AB In 1989, we initiated a long-term field experiment in a semiarid thorn scrub community in north-central Chile. We posited that biotic interactions, particularly predation, interspecific competition, and herbivory assumed a primary top-down role in affecting small mammals and annual plants here. Using a multi factorial design we selectively excluded vertebrate predators (principally carnivores and raptors) and a large small mammal herbivore, the degu (Octodon degus), from replicated 0.56 ha exclosures located in a valley near the coast in Bosque Fray Jorge National Park. Evidence initially supported effects of predator exclusion on O. degus but not for other small mammals in the assemblage (e.g., Phyllotis darwini, Abrothrix olivaceus). Subsequent years of monitoring have documented that predation has temporary effects on degu numbers, but that populations of this and other small mammals are much more strongly influenced by environmental bottom-up factors (i.e., rainfall). Further, our experimental manipulation has provided no evidence for negative interspecific competition effects on numbers of any small mammal in this assemblage. Degu exclusions, however, have had negative indirect effects on exotic annuals; native annuals appear to outcompete exotics especially during drought years The effect is magnified in all-small mammal exclusions. Since about 2002, selective exclusions of lagomorphs and small mammals have resulted in changes in cover of some perennial shrubs. Also notable has been a fundamental shift in the small mammal composition following the last major El Nino Southern Oscillation (ENSO) event in 2000 2002; degus now comprise a majority of small mammal biomass in the assemblage, and their numbers have become more stable and less temporally variable. This appears to have been caused by a shift in rainfall periodicity from strong interannual fluctuations in response to periodic ENSOs, to a more equitable pattern with more consistent annual rainfall. This represents one of the first documented cases of system-wide biotic phase shifts to a relatively modest change in rainfall regime. This may be indicative of ongoing climate change in the Chilean semiarid region, and we expect that further changes in the community will occur if those trends continue. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Meserve, Peter L.] No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA. [Kelt, Douglas A.] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, Davis, CA 95616 USA. [Gutierrez, Julio R.] Univ La Serena, Casilla 599, La Serena, Chile. [Gutierrez, Julio R.] CEAZA, Casilla 599, La Serena, Chile. [Gutierrez, Julio R.] IEB, Casilla 653, Santiago, Chile. [Andrea Previtali, M.] Univ Nacl Litoral, Fac Humanidades & Ciencias, Dept Ciencias Nat, RA-3000 Santa Fe, Argentina. [Andrea Previtali, M.] Consejo Nacl Invest Cient & Tecn, RA-3000 Santa Fe, Argentina. [Milstead, W. Bryan] US EPA, Natl Hlth & Environm Effects Lab, Atlantic Ecol Div, Narragansett, RI 02882 USA. [Meserve, Peter L.] Univ Idaho, Dept Biol Sci, Moscow, ID 83844 USA. RP Meserve, PL (reprint author), No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA.; Meserve, PL (reprint author), Univ Idaho, Dept Biol Sci, Moscow, ID 83844 USA. EM pmeserve@uidaho.edu FU NSF-LTREB DEB [1456729]; FONDECYT Chile; U.S. AID Program; Northern Illinois University; University of California, Davis FX We are grateful to many sources of financial support for this project over 26 years including the U.S. National Science Foundation (most recently NSF-LTREB DEB 1456729 to DAK and PLM), FONDECYT Chile, the U.S. AID Program, Northern Illinois University, and the University of California, Davis. Many people too numerous to mention have contributed to the project. Finally, we gratefully acknowledge the cooperation of the Corporation Nacional Forestal (CONAF) in Chile which has allowed us to transform the study area to a living laboratory. NR 70 TC 4 Z9 4 U1 8 U2 28 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD MAR PY 2016 VL 126 SI SI BP 81 EP 88 DI 10.1016/j.jaridenv.2015.08.016 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DD7JT UT WOS:000370101200011 ER PT J AU Oliver, P AF Oliver, Priscilla TI NEHA SECOND VICE PRESIDENTIAL CANDIDATE PROFILE SO JOURNAL OF ENVIRONMENTAL HEALTH LA English DT Editorial Material C1 [Oliver, Priscilla] US EPA, Off Reg Adm, Atlanta, GA USA. [Oliver, Priscilla] Univ Alabama, Tuscaloosa, AL USA. RP Oliver, P (reprint author), US EPA, Off Reg Adm, Atlanta, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATL ENVIRON HEALTH ASSOC PI DENVER PA 720 S COLORADO BLVD SUITE 970, SOUTH TOWER, DENVER, CO 80246 USA SN 0022-0892 J9 J ENVIRON HEALTH JI J. Environ. Health PD MAR PY 2016 VL 78 IS 7 BP 52 EP 52 PG 1 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DD7JS UT WOS:000370101100009 ER PT J AU Rudraiah, S Gu, XS Hines, RN Manautou, JE AF Rudraiah, Swetha Gu, Xinsheng Hines, Ronald N. Manautou, Jose E. TI Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells SO TOXICOLOGY IN VITRO LA English DT Article DE Flavin-containing monoxygenase-3; Oxidative stress; NRF2; KEAP1; Tert-butyl hydroperoxide; Tert-butyl hydroquinone ID ACETAMINOPHEN HEPATOTOXICITY; FLAVIN-CONTAINING-MONOOXYGENASE-3 FMO3; LIVER; INDUCTION; MICE; AUTOPROTECTION; POLYMORPHISMS; METABOLISM; TOLERANCE; MECHANISM AB The flavin-containing monooxygenases (FMOs) are important for the oxidation of a variety of endogenous compounds and xenobiotics. The hepatic expression of FMO3 is highly variable and until recently, it was thought to be uninducible. In this study, human FMO3 gene regulation by the oxidative stress transcription factor, nuclear factor (erythroid-derived 2)-like 2 (NRF2) was examined. Constitutive FMO3 gene expression is repressed in HepG2 cells, thus this cell can be a good model for FMO3 gene regulation studies. Over-expression of NRF2 in HepG2 cells increased NRF2 target gene expression, heme oxygenase-1 (HMOX1) and NAD(P)H:quinone oxidoreductase-1 (NQO1), but did not alter FMO3 gene expression. Co-transfection studies with NRF2 or its cytosolic regulatory protein, Kelch-like ECH-associated protein 1 (KEAP1), expression vectors, along with FMO3 promoter luciferase reporter constructs of various lengths (5 kb or 6 kb), did not change FMO3 reporter gene activity significantly. Furthermore, treatment with tert-butyl hydroperoxide (tBHP) and tert-butyl hydroquinone (tBHQ) did not alter FMO3 reporter construct activity. In summary, in vitro results suggest that the transcriptional regulation of FMO3 might not involve the NRF2-KEAP1 regulatory pathway. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Rudraiah, Swetha; Gu, Xinsheng; Manautou, Jose E.] Univ Connecticut, Dept Pharmaceut Sci, Storrs, CT USA. [Hines, Ronald N.] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. Univ Connecticut, Toxicol Program, Dept Pharmaceut Sci, Sch Pharm, Storrs, CT 06269 USA. RP Manautou, JE (reprint author), Univ Connecticut, Toxicol Program, Dept Pharmaceut Sci, Sch Pharm, 69 North Eagleville Rd,Unit 3092, Storrs, CT 06269 USA. EM swetha.rudraiah@uconn.edu; gu.xinsheng@gmail.com; hines.ronald@epa.gov; jose.manautou@uconn.edu OI Hines, Ronald/0000-0002-3094-4200 FU National Institutes of Health [DK069557] FX This work is supported by the National Institutes of Health Grant (DK069557). NR 22 TC 0 Z9 0 U1 2 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0887-2333 J9 TOXICOL IN VITRO JI Toxicol. Vitro PD MAR PY 2016 VL 31 BP 54 EP 59 DI 10.1016/j.tiv.2015.11.016 PG 6 WC Toxicology SC Toxicology GA DD4GE UT WOS:000369879700007 PM 26616280 ER PT J AU Garrido-Baserba, M Asvapathanagul, P McCarthy, GW Gocke, TE Olson, BH Park, HD Al-Omari, A Murthy, S Bott, CB Wett, B Smeraldi, JD Shaw, AR Rosso, D AF Garrido-Baserba, Manel Asvapathanagul, Pitiporn McCarthy, Graham W. Gocke, Thomas E. Olson, Betty H. Park, Hee-Deung Al-Omari, Ahmed Murthy, Sudhir Bott, Charles B. Wett, Bernhard Smeraldi, Joshua D. Shaw, Andrew R. Rosso, Diego TI Linking biofilm growth to fouling and aeration performance of fine-pore diffuser in activated sludge SO WATER RESEARCH LA English DT Article DE Aeration; Fouling; Fine-pore diffuser; Biofilm; Activated sludge; Efficiency ID SUBMERGED MEMBRANE BIOREACTOR; MUNICIPAL WASTE-WATER; OXYGEN-TRANSFER; ALPHA-FACTOR; TRANSFER EFFICIENCY; SYSTEMS; SCALE; SPECIFICATION; COMMUNITIES; MECHANISMS AB Aeration is commonly identified as the largest contributor to process energy needs in the treatment of wastewater and therefore garners significant focus in reducing energy use. Fine-pore diffusers are the most common aeration system in municipal wastewater treatment. These diffusers are subject to fouling and scaling, resulting in loss in transfer efficiency as biofilms form and change material properties producing larger bubbles, hindering mass transfer and contributing to increased plant energy costs. This research establishes a direct correlation and apparent mechanistic link between biofilm DNA concentration and reduced aeration efficiency caused by biofilm fouling. Although the connection between biofilm growth and fouling has been implicit in discussions of diffuser fouling for many years, this research provides measured quantitative connection between the extent of biofouling and reduced diffuser efficiency. This was clearly established by studying systematically the deterioration of aeration diffusers efficiency during a 1.5 year period, concurrently with the microbiological study of the biofilm fouling in order to understand the major factors contributing to diffuser fouling. The six different diffuser technologies analyzed in this paper included four different materials which were ethylene-propylenediene monomer (EPDM), polyurethane, silicone and ceramic. While all diffusers foul eventually, some novel materials exhibited fouling resistance. The material type played a major role in determining the biofilm characteristics (i.e., growth rate, composition, and microbial density) which directly affected the rate and intensity at what the diffusers were fouled, whereas diffuser geometry exerted little influence. Overall, a high correlation between the increase in biofilm DNA and the decrease in alpha F was evident (CV < 14.0 +/- 2.0%). By linking bacterial growth with aeration efficiency, the research was able to show quantitatively the causal connection between bacterial fouling and energy wastage during aeration. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Garrido-Baserba, Manel; Asvapathanagul, Pitiporn; McCarthy, Graham W.; Gocke, Thomas E.; Olson, Betty H.; Rosso, Diego] Univ Calif Irvine, Dept Civil & Environm Engn, Irvine, CA 92697 USA. [Asvapathanagul, Pitiporn] Calif State Univ Long Beach, Dept Civil Engn & Construct Engn Management, Long Beach, CA 90840 USA. [Olson, Betty H.; Rosso, Diego] Univ Calif Irvine, Water Energy Nexus Ctr, Irvine, CA 92697 USA. [Park, Hee-Deung] Korea Univ, Sch Civil Environm & Architectural Engn, Seoul 136713, South Korea. [Al-Omari, Ahmed; Murthy, Sudhir] DC Water, 5000 Overlook Ave SW, Washington, DC 20032 USA. [Bott, Charles B.] Hampton Rd Sanitat Dist, Virginia Beach, VA 23471 USA. [Wett, Bernhard] ARAconsult, Unterbergerstr 1, A-6020 Innsbruck, Austria. [Smeraldi, Joshua D.] US EPA, 1200 Penn Ave NW, Washington, DC 20460 USA. [Shaw, Andrew R.] Black & Veatch Consulting Engineers, 8400 Ward Pkwy, Kansas City, MO 64114 USA. RP Garrido-Baserba, M; Rosso, D (reprint author), Univ Calif Irvine, Dept Civil & Environm Engn, Irvine, CA 92697 USA. EM manelg@uci.edu; bidui@uci.edu OI Garrido-Baserba, Manel/0000-0002-3603-710X FU Water Environment Research Foundation [INFR2R12]; United States Environmental Protection Agency; Irvine Ranch Water District; Hampton Roads Sanitation District; DC Water; Southern California Edison FX This research was funded by the Water Environment Research Foundation (contract number INFR2R12) and the United States Environmental Protection Agency, with the support of the Irvine Ranch Water District, Hampton Roads Sanitation District, DC Water, and Southern California Edison. The authors thank David M. Hayden of the Irvine Ranch Michelson Water Reclamation Plant for the invaluable help and Alice K. Robinson of BKT for the help during field work. We thank Taek-Seung Kim for the EDX analyses. NR 49 TC 4 Z9 4 U1 9 U2 36 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD MAR 1 PY 2016 VL 90 BP 317 EP 328 DI 10.1016/j.watres.2015.12.011 PG 12 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DD7GP UT WOS:000370092000033 PM 26760484 ER PT J AU Geron, C Daly, R Harley, P Rasmussen, R Seco, R Guenther, A Karl, T Gu, LH AF Geron, Chris Daly, Ryan Harley, Peter Rasmussen, Rei Seco, Roger Guenther, Alex Karl, Thomas Gu, Lianhong TI Large drought-induced variations in oak leaf volatile organic compound emissions during PINOT NOIR 2012 SO CHEMOSPHERE LA English DT Article DE Isoprene; Monoterpenes; Drought; Ozarks; Biogenic emissions; MEGAN ID ISOPRENE EMISSION; LIQUIDAMBAR-STYRACIFLUA; STOMATAL CONDUCTANCE; CENTRAL MISSOURI; WATER RELATIONS; SUMMER DROUGHT; SOIL DROUGHT; PHOTOSYNTHETIC PERFORMANCE; QUERCUS-PUBESCENS; POPULUS-DELTOIDES AB Leaf-level isoprene and monoterpene emissions were collected and analyzed from five of the most abundant oak (Quercus) species in Central Missouri's Ozarks Region in 2012 during PINOT NOIR (Particle Investigations at a Northern Ozarks Tower NOx, Oxidants, Isoprene Research). June measurements, prior to the onset of severe drought, showed isoprene emission rates and leaf temperature responses similar to those previously reported in the literature and used in Biogenic Volatile Organic Compound (BVOC) emission models. During the peak of the drought in August, isoprene emission rates were substantially reduced, and response to temperature was dramatically altered, especially for the species in the red oak subgenus (Erythrobalanus). Quercus stellata (in the white oak subgenus Leucobalanus), on the other hand, increased its isoprene emission rate during August, and showed no decline at high temperatures during June or August, consistent with its high tolerance to drought and adaptation to xeric sites at the prairie deciduous forest interface. Mid-late October measurements were conducted after soil moisture recharge, but were affected by senescence and cooler temperatures. Isoprene emission rates were considerably lower from all species compared to June and August data. The large differences between the oaks in response to drought emphasizes the need to consider BVOC emissions at the species level instead of just the whole canopy. Monoterpene emissions from Quercus rubra in limited data were highest among the oaks studied, while monoterpene emissions from the other oak species were 80-95% lower and less than assumed in current BVOC emission models. Major monoterpenes from Q rubra (and in ambient air) were p-cymene, alpha-pinene, beta-pinene, D-limonene, gamma-terpinene, beta-ocimene (predominantly1,3,7-trans-(beta-ocimene, but also 1,3,6-trans-beta-ocimene), tricyclene, alpha-terpinene, sabinene, terpinolene, and myrcene. Results are discussed in the context of canopy flux studies conducted at the site during PINOT NOIR, which are described elsewhere. The leaf isoprene emissions before and during the drought were consistent with above canopy fluxes, while leaf and branch monoterpene emissions were an order of magnitude lower than the observed above canopy fluxes, implying that other sources may be contributing substantially to monoterpene fluxes at this site. This strongly demonstrates the need for further simultaneous canopy and enclosure BVOC emission studies. Published by Elsevier Ltd. C1 [Geron, Chris; Daly, Ryan] US EPA, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. [Harley, Peter] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. [Rasmussen, Rei] Oregon Grad Inst, Portland, OR USA. [Seco, Roger; Guenther, Alex] Univ Calif Irvine, Dept Earth Syst Sci, Irvine, CA 92697 USA. [Karl, Thomas] Univ Innsbruck, Inst Meteorol & Geophys, A-6020 Innsbruck, Austria. [Gu, Lianhong] Oak Ridge Natl Lab, Oak Ridge, TN USA. RP Geron, C (reprint author), US EPA, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA. EM geron.chris@epa.gov RI Seco, Roger/F-7124-2011; Karl, Thomas/D-1891-2009; Gu, Lianhong/H-8241-2014 OI Geron, Chris/0000-0002-4266-2155; Seco, Roger/0000-0002-2078-9956; Karl, Thomas/0000-0003-2869-9426; Gu, Lianhong/0000-0001-5756-8738 FU Fundacion Ramon Areces FX We gratefully acknowledge the support of Kevin Hosman for logistic support at the BREA site. Dr. Steve Pallardy also provided access and technical support at the site and also provided the forest inventory data for the MOFLUX tower footprint. Dr. Paul Hanson of Oak Ridge National Laboratory provided information on soil characteristics near the tower. RS was partly supported by a postdoctoral fellowship awarded by Fundacion Ramon Areces. This research has been subjected to administrative review by the United States Environmental Protection Agency and approved for publication. NR 78 TC 0 Z9 0 U1 10 U2 35 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD MAR PY 2016 VL 146 BP 8 EP 21 DI 10.1016/j.chemosphere.2015.11.086 PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA DC8GD UT WOS:000369457000002 PM 26706927 ER PT J AU Shao, Y Lunetta, RS Wheeler, B Iiames, JS Campbell, JB AF Shao, Yang Lunetta, Ross S. Wheeler, Brandon Iiames, John S. Campbell, James B. TI An evaluation of time-series smoothing algorithms for land-cover classifications using MODIS-NDVI multi-temporal data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE MODIS-NDVI; Multi-temporal analysis; Smoothing algorithms; Validation ID GREAT-LAKES BASIN; SATELLITE SENSOR DATA; VEGETATION PHENOLOGY; AVHRR DATA; EXTRACTION; ACCURACY; CROPLAND; IMAGES AB In this study we compared the Savitzky-Golay, asymmetric Gaussian, double-logistic, Whittaker smoother, and discrete Fourier transformation smoothing algorithms (noise reduction) applied to Moderate Resolution Imaging Spectroradiometer (MODIS) Normalized Difference Vegetation Index (NDVI) time-series data, to provide continuous phenology data used for land-cover (LC) classifications across the Laurentian Great Lakes Basin (GLB). MODIS 16-day 250 m NDVI imagery for the GLB was used in conjunction with National Land Cover Database (NLCD) from 2001, 2006 and 2011, and the Cropland Data Layers (CDL) from 2011 to 2014 to conduct classification evaluations. Inter-class separability was measured by Jeffries-Matusita (JM) distances between selected cover type pairs (both general classes and specific crops), and intra-class variability was measured by calculating simple Euclidean distance for samples within cover types. For the GLB, we found that the application of a smoothing algorithm significantly reduced image noise compared to the raw data. However, the Jeffries-Matusita (JM) measures for smoothed NDVI temporal profiles resulted in large inconsistencies. Of the five algorithms tested, only the Fourier transformation algorithm and Whittaker smoother improved inter-class separability for corn soybean class pair and significantly improved overall classification accuracy. When compared to the raw NDVI data as input, the overall classification accuracy from the Fourier transformation and Whittaker smoother improved performance by approximately 2-6% for some years. Conversely, the asymmetric Gaussian and double-logistic smoothing algorithms actually led to degradation of classification performance. (C) 2015 Elsevier Inc. All rights reserved. C1 [Shao, Yang; Wheeler, Brandon; Campbell, James B.] Virginia Tech, Coll Nat Resources & Environm, Dept Geog, 115 Major Williams Hall, Blacksburg, VA 24061 USA. [Lunetta, Ross S.; Iiames, John S.] US EPA, Natl Exposure Res Lab, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. RP Shao, Y (reprint author), Virginia Tech, Coll Nat Resources & Environm, Dept Geog, 115 Major Williams Hall, Blacksburg, VA 24061 USA. EM yshao@vt.edu FU U.S. EPA's Office of Research and Development; NASA's Ocean Biology and Biogeochemical Program [NNH15AZ42I] FX The U.S. Environmental Protection Agency partially conducted the research described in this paper. Although this work was reviewed by EPA and has been approved for publication, it may not necessarily reflect official Agency policy. Mention of any trade names or commercial products does not constitute endorsement or recommendation for use. This research was partially funded by the U.S. EPA's Office of Research and Development and NASA's Ocean Biology and Biogeochemical Program under grant NNH15AZ42I. NR 37 TC 9 Z9 10 U1 9 U2 45 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAR 1 PY 2016 VL 174 BP 258 EP 265 DI 10.1016/j.rse.2015.12.023 PG 8 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DB8DI UT WOS:000368746800020 ER PT J AU Zhang, YZ Wang, YH Chen, G Smeltzer, C Crawford, J Olson, J Szykman, J Weinheimer, AJ Knapp, DJ Montzka, DD Wisthaler, A Mikoviny, T Fried, A Diskin, G AF Zhang, Yuzhong Wang, Yuhang Chen, Gao Smeltzer, Charles Crawford, James Olson, Jennifer Szykman, James Weinheimer, Andrew J. Knapp, David J. Montzka, Denise D. Wisthaler, Armin Mikoviny, Tomas Fried, Alan Diskin, Glenn TI Large vertical gradient of reactive nitrogen oxides in the boundary layer: Modeling analysis of DISCOVER-AQ 2011 observations SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE NOx; 1-D model; boundary layer; vertical distribution; ozone production rate; satellite retrieval ID OZONE MONITORING INSTRUMENT; TROPOSPHERIC NO2; ATMOSPHERIC OXIDATION; CONVECTIVE-TRANSPORT; SATELLITE RETRIEVALS; COLUMN RETRIEVAL; LIGHTNING NOX; NORTH-AMERICA; CLOSURE-MODEL; MEXICO-CITY AB An often used assumption in air pollution studies is a well-mixed boundary layer (BL), where pollutants are evenly distributed. Because of the difficulty in obtaining vertically resolved measurements, the validity of the assumption has not been thoroughly evaluated. In this study, we use more than 200 vertical profiles observed in the Deriving Information on Surface Conditions from Column and Vertically Resolved Observations Relevant to Air Quality (DISCOVER-AQ) aircraft campaign in July 2011 to examine the vertical distributions of pollutants over the Washington-Baltimore area. While many long-lived species are well mixed in daytime, the observed average vertical profile of NOx shows a large negative gradient with increasing altitude in the BL. Our analysis suggests that the magnitude of the NOx gradient is highly sensitive to atmospheric stability. We investigate how parameterizations of the BL and land-surface processes impact vertical profiles in a 1-D chemical transport model, using three BL schemes (Asymmetric Convective Model version 2 (ACM2), Yonsei University (YSU), and Mellor-Yamada-Janjic (MYJ)) and two land-surface schemes (Noah and Rapid Update Cycle (RUC)). The model reasonably reproduces the median vertical profiles of NOx under different BL stability conditions within 30% of observations, classified based on potential temperature gradient and BL height. Comparisons with NOx observations for individual vertical profiles reveal that while YSU performs better in the turbulent and deep BL case, in general, ACM2 (RMSE=2.0ppbv) outperforms YSU (RMSE=2.5ppbv) and MYJ (RMSE=2.2ppbv). Results also indicate that the land-surface schemes in the Weather Research and Forecasting (WRF) model have a small impact on the NOx gradient. Using model simulations, we analyze the impact of BL NOx gradient on the calculation of the ozone production rate and satellite NO2 retrieval. We show that using surface measurements and the well-mixed BL assumption causes a similar to 45% high bias in the estimated BL ozone production rate and that the variability of NO2 vertical profiles is responsible for 5-10% variability in the retrieved NO2 tropospheric vertical columns. C1 [Zhang, Yuzhong; Wang, Yuhang; Smeltzer, Charles] Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA. [Chen, Gao; Crawford, James; Olson, Jennifer; Szykman, James; Diskin, Glenn] NASA, Langley Res Ctr, Hampton, VA 23665 USA. [Szykman, James] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Weinheimer, Andrew J.; Knapp, David J.; Montzka, Denise D.] Natl Ctr Atmospher Res, Div Atmospher Chem, Boulder, CO 80307 USA. [Wisthaler, Armin] Univ Innsbruck, Inst Ionenphys & Angew Phys, A-6020 Innsbruck, Austria. [Wisthaler, Armin; Mikoviny, Tomas] Univ Oslo, Dept Chem, Oslo, Norway. [Mikoviny, Tomas] Oak Ridge Associated Univ, Oak Ridge, TN USA. [Fried, Alan] Univ Colorado, Boulder, CO 80309 USA. RP Zhang, YZ (reprint author), Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA. EM yzhang425@gatech.edu FU NASA ACMAP program; NASA DISCOVER-AQ program; NASA Postdoctoral Program at the Langley Research Center; NASA FX The data for this paper are available at the DISCOVER-AQ data archive (http://www-air.larc.nasa.gov/missions/discover-aq/discover-aq.html). The research was supported by the NASA ACMAP and DISCOVER-AQ programs. We thank David Parrish for his discussion with Y.W. that led to the analyses reported here. PTR-MS measurements of VOCs were supported by the Austrian Federal Ministry for Transport, Innovation, and Technology (BMVIT) through the Austrian Space Applications Programme (ASAP) of the Austrian Research Promotion Agency (FFG). The work of T.M. was supported by an appointment to the NASA Postdoctoral Program at the Langley Research Center administered by Oak Ridge Associated Universities through a contract with NASA. NR 66 TC 4 Z9 4 U1 8 U2 21 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD FEB 27 PY 2016 VL 121 IS 4 BP 1922 EP 1934 DI 10.1002/2015JD024203 PG 13 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DH7MK UT WOS:000372977900034 ER PT J AU Canedo-Arguelles, M Hawkins, CP Kefford, BJ Schafer, RB Dyack, BJ Brucet, S Buchwalter, D Dunlop, J Fror, O Lazorchak, J Coring, E Fernandez, HR Goodfellow, W Achem, ALG Hatfield-Dodds, S Karimov, BK Mensah, P Olson, JR Piscart, C Prat, N Ponsa, S Schulz, CJ Timpano, AJ AF Canedo-Argueelles, M. Hawkins, C. P. Kefford, B. J. Schaefer, R. B. Dyack, B. J. Brucet, S. Buchwalter, D. Dunlop, J. Froer, O. Lazorchak, J. Coring, E. Fernandez, H. R. Goodfellow, W. Gonzalez Achem, A. L. Hatfield-Dodds, S. Karimov, B. K. Mensah, P. Olson, J. R. Piscart, C. Prat, N. Ponsa, S. Schulz, C. -J. Timpano, A. J. TI Saving freshwater from salts SO SCIENCE LA English DT Editorial Material ID RIVERS C1 [Canedo-Argueelles, M.; Brucet, S.; Ponsa, S.] Univ Vic Cent Univ Catalonia, Vic 08500, Spain. [Canedo-Argueelles, M.; Prat, N.] Univ Barcelona, E-08028 Barcelona, Spain. [Hawkins, C. P.] Utah State Univ, Logan, UT 84322 USA. [Kefford, B. J.; Dyack, B. J.] Univ Canberra, Canberra, ACT 2601, Australia. [Schaefer, R. B.; Froer, O.] Univ Koblenz Landau, D-76829 Landau, Germany. [Brucet, S.] Catalan Inst Res & Adv Studies, Barcelona 08010, Spain. [Buchwalter, D.] N Carolina State Univ, Raleigh, NC 27695 USA. [Dunlop, J.] Queensland Govt, Brisbane, Qld, Australia. [Lazorchak, J.] US EPA, Cincinnati, OH 45268 USA. [Coring, E.] EcoRing, D-37181 Hardegsen, Germany. [Fernandez, H. R.; Gonzalez Achem, A. L.] Inst Biodiversidad Neotrop, San Miguel De Tucuman, Argentina. [Goodfellow, W.] Exponent Inc, Alexandria, VA 22314 USA. [Hatfield-Dodds, S.] CSIRO Land & Water, Canberra, ACT 2601, Australia. [Karimov, B. K.] Uzbek Acad Sci, Tashkent, Uzbekistan. [Mensah, P.] Rhodes Univ, ZA-6140 Grahamstown, South Africa. [Olson, J. R.] Desert Res Inst, Las Vegas, NV 89119 USA. [Piscart, C.] Univ Rennes, F-35042 Rennes, France. [Schulz, C. -J.] Thuringian State Inst Environm & Geol, D-07745 Jena, Germany. [Timpano, A. J.] Virginia Polytech Inst & State Univ, Blacksburg, VA 24061 USA. RP Canedo-Arguelles, M (reprint author), Univ Vic Cent Univ Catalonia, Vic 08500, Spain. EM mcanedo.fem@gmail.com RI Schaefer, Ralf/E-1926-2011; Hawkins, Charles/A-4530-2008; Prat, Narcis /D-4405-2014; Canedo-Arguelles, Miguel/L-6365-2014; Hatfield-Dodds, Steve/K-1067-2013 OI Schaefer, Ralf/0000-0003-3510-1701; Hawkins, Charles/0000-0003-1247-0248; Prat, Narcis /0000-0002-1550-1305; Canedo-Arguelles, Miguel/0000-0003-3864-7451; NR 17 TC 10 Z9 10 U1 25 U2 55 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD FEB 26 PY 2016 VL 351 IS 6276 BP 914 EP 916 DI 10.1126/science.aad3488 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DE7MO UT WOS:000370821400018 PM 26917752 ER PT J AU Rodriguez-Jorquera, IA Silva-Sanchez, C Strynar, M Denslow, ND Toor, GS AF Rodriguez-Jorquera, Ignacio A. Silva-Sanchez, Cecilia Strynar, Mark Denslow, Nancy D. Toor, Gurpal S. TI Footprints of Urban Micro-Pollution in Protected Areas: Investigating the Longitudinal Distribution of Perfluoroalkyl Acids in Wildlife Preserves SO PLOS ONE LA English DT Article ID WASTE-WATER TREATMENT; PERFLUOROOCTANE SULFONATE PFOS; PERFLUORINATED COMPOUNDS; TREATMENT PLANTS; QUANTITATIVE-DETERMINATION; RIVER-BASIN; TOKYO BAY; SURFACTANTS; SEDIMENTS; FATE AB Current approaches to protect biodiversity by establishing protected areas usually gloss over water pollution as a threat. Our objective was to determine the longitudinal and seasonal distribution of perfluoroalkyl acids (PFAAs) in water column and sediments from a wastewater dominated stream that enters preservation areas. Water samples were collected along the longitudinal section (six sites, 1000 m away from each other) of the stream during the dry and wet seasons. Sediments were collected from three sites along the stream from three depths. Water and sediments were analyzed for PFAAs using high performance liquid chromatography-tandem mass spectrometry. Eleven PFAAs with 5 to 14 carbon atoms were detected in the water column at all sampling points, with a minor reduction at the last point suggesting a dilution effect. The most detected PFAAs was PFOS, followed by perfluorooctanoic acid (PFOA), and perfluorohexanoic acid (PFHxA). Seasonal differences in PFAAs concentrations suggested contribution of stormwater runoff during the wet season. All analyzed PFAAs in sediments were under the limit of quantification, likely due to the high proportion of sand and low organic matter. However, high concentrations of PFAAs were detected in the water column inside the protected areas, which includes PFOS in concentrations considered not safe for avian wildlife. Water samples appear to be more relevant than sediments to determine PFAAs micro-pollution in water bodies with sandy sediments. Inclusion of a management plans on micro-pollution research, monitoring, and mitigation is recommended for protected areas. C1 [Rodriguez-Jorquera, Ignacio A.] Univ Florida, Sch Nat Resources & Environm, Soil & Water Sci Dept, Interdisciplinary Ecol Program, Gainesville, FL USA. [Rodriguez-Jorquera, Ignacio A.; Denslow, Nancy D.] Univ Florida, Dept Physiol Sci, Gainesville, FL 32610 USA. [Rodriguez-Jorquera, Ignacio A.; Denslow, Nancy D.] Univ Florida, Ctr Environm & Human Toxicol, Gainesville, FL USA. [Silva-Sanchez, Cecilia] Univ Florida, Interdisciplinary Ctr Biotechnol Res, Prote & Mass Spectrometry, Gainesville, FL USA. [Strynar, Mark] US EPA, Natl Exposure Res Lab, Durham, NC USA. [Toor, Gurpal S.] Univ Florida, Inst Food & Agr Sci, Gulf Coast Res & Educ Ctr, Soil & Water Qual Lab, Wimauma, FL USA. [Rodriguez-Jorquera, Ignacio A.] Univ Florida, Dept Wildlife Ecol & Conservat, Gainesville, FL USA. RP Rodriguez-Jorquera, IA (reprint author), Univ Florida, Sch Nat Resources & Environm, Soil & Water Sci Dept, Interdisciplinary Ecol Program, Gainesville, FL USA.; Rodriguez-Jorquera, IA (reprint author), Univ Florida, Dept Physiol Sci, Gainesville, FL 32610 USA.; Rodriguez-Jorquera, IA (reprint author), Univ Florida, Ctr Environm & Human Toxicol, Gainesville, FL USA.; Toor, GS (reprint author), Univ Florida, Inst Food & Agr Sci, Gulf Coast Res & Educ Ctr, Soil & Water Qual Lab, Wimauma, FL USA.; Rodriguez-Jorquera, IA (reprint author), Univ Florida, Dept Wildlife Ecol & Conservat, Gainesville, FL USA. EM irodriguezj@ufl.edu; gstoor@ufl.edu FU Soil and Water Quality Laboratory located at the Gulf Coast Research and Education Center, University of Florida; BecasChile Conicyt; CONCIYT-Chile; Soil and Water Quality Laboratory located at the Gulf Coast Research and Education Center of the University of Florida-Institute of Food and Agricultural Sciences FX Funding for this research was provided by the Soil and Water Quality Laboratory located at the Gulf Coast Research and Education Center, University of Florida. BecasChile Conicyt provided a graduate research fellowship to IARJ.; We acknowledge the help of Kevin Kroll, Viet Dang, Cristina Colli-Dulla, Sixue Chen, Todd Osborne, and Claudio Verdugo during various phases of this research. The first author thank CONCIYT-Chile for providing a graduate student fellowship for PhD study at University of Florida. Funding for this research was provided by Soil and Water Quality Laboratory located at the Gulf Coast Research and Education Center of the University of Florida-Institute of Food and Agricultural Sciences. NR 65 TC 2 Z9 2 U1 7 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 24 PY 2016 VL 11 IS 2 AR e0148654 DI 10.1371/journal.pone.0148654 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF2IA UT WOS:000371164700012 PM 26909512 ER PT J AU Cohen, MJ Creed, IF Alexander, L Basu, NB Calhoun, AJK Craft, C D'Amico, E DeKeyser, E Fowler, L Golden, HE Jawitz, JW Kalla, P Kirkman, LK Lane, CR Lang, M Leibowitz, SG Lewis, DB Marton, J McLaughlin, DL Mushet, DM Raanan-Kiperwas, H Rains, MC Smith, L Walls, SC AF Cohen, Matthew J. Creed, Irena F. Alexander, Laurie Basu, Nandita B. Calhoun, Aram J. K. Craft, Christopher D'Amico, Ellen DeKeyser, Edward Fowler, Laurie Golden, Heather E. Jawitz, James W. Kalla, Peter Kirkman, L. Katherine Lane, Charles R. Lang, Megan Leibowitz, Scott G. Lewis, David Bruce Marton, John McLaughlin, Daniel L. Mushet, David M. Raanan-Kiperwas, Hadas Rains, Mark C. Smith, Lora Walls, Susan C. TI Do geographically isolated wetlands influence landscape functions? SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE connectivity; navigable waters; significant nexus ID FRESH-WATER WETLANDS; PRAIRIE POTHOLE REGION; SPECIES RICHNESS; HYDROLOGIC CONNECTIVITY; FORESTED LANDSCAPES; UNITED-STATES; VERNAL POOLS; BIOGEOCHEMICAL PROCESSES; CONSTRUCTED WETLANDS; FLOODPLAIN HABITATS AB Geographically isolated wetlands (GIWs), those surrounded by uplands, exchange materials, energy, and organisms with other elements in hydrological and habitat networks, contributing to landscape functions, such as flow generation, nutrient and sediment retention, and biodiversity support. GIWs constitute most of the wetlands in many North American landscapes, provide a disproportionately large fraction of wetland edges where many functions are enhanced, and form complexes with other water bodies to create spatial and temporal heterogeneity in the timing, flow paths, and magnitude of network connectivity. These attributes signal a critical role for GIWs in sustaining a portfolio of landscape functions, but legal protections remain weak despite preferential loss from many landscapes. GIWs lack persistent surface water connections, but this condition does not imply the absence of hydrological, biogeochemical, and biological exchanges with nearby and downstream waters. Although hydrological and biogeochemical connectivity is often episodic or slow (e.g., via groundwater), hydrologic continuity and limited evaporative solute enrichment suggest both flow generation and solute and sediment retention. Similarly, whereas biological connectivity usually requires overland dispersal, numerous organisms, including many rare or threatened species, use both GIWs and downstream waters at different times or life stages, suggesting that GIWs are critical elements of landscape habitat mosaics. Indeed, weaker hydrologic connectivity with downstream waters and constrained biological connectivity with other landscape elements are precisely what enhances some GIW functions and enables others. Based on analysis of wetland geography and synthesis of wetland functions, we argue that sustaining landscape functions requires conserving the entire continuum of wetland connectivity, including GIWs. C1 [Cohen, Matthew J.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Creed, Irena F.] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada. [Alexander, Laurie] US EPA, Natl Ctr Environm Assessment, Washington, DC 20460 USA. [Basu, Nandita B.] Univ Waterloo, Dept Earth & Environm Sci, Waterloo, ON N2L 3G1, Canada. [Calhoun, Aram J. K.] Univ Maine, Dept Wildlife Fisheries & Conservat Biol, Orono, ME 04469 USA. [Craft, Christopher] Indiana Univ, Sch Publ Hlth & Environm Affairs, Bloomington, IN 47405 USA. [D'Amico, Ellen] CSS Dynamac Corp, Cincinnati, OH 45268 USA. [DeKeyser, Edward] N Dakota State Univ, Sch Nat Resource Sci, Fargo, ND 58105 USA. [Fowler, Laurie] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Golden, Heather E.; Lane, Charles R.] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [Jawitz, James W.] Univ Florida, Soil & Water Sci Dept, Gainesville, FL 32611 USA. [Kalla, Peter] US EPA, Reg 4, Athens, GA 30605 USA. [Kirkman, L. Katherine; Smith, Lora] Joseph W Jones Ecol Res Ctr, Newton, GA 39870 USA. [Lang, Megan] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Leibowitz, Scott G.] US EPA, Western Ecol Div, Natl Hlth & Environm Effects Res Lab, Corvallis, OR 97333 USA. [Lewis, David Bruce] Univ S Florida, Dept Integrat Biol, Tampa, FL 33620 USA. [Marton, John] CDM Smith Inc, Indianapolis, IN 46204 USA. [McLaughlin, Daniel L.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Mushet, David M.] US Geol Survey, Prairie & No Wildlife Res Ctr, Jamestown, ND 58401 USA. [Raanan-Kiperwas, Hadas] US EPA, Off Wetlands Oceans & Watersheds, Washington, DC 20460 USA. [Rains, Mark C.] Univ S Florida, Sch Geosci, Tampa, FL USA. [Walls, Susan C.] US Geol Survey, Wetland & Aquat Res Ctr, Gainesville, FL 32653 USA. RP Cohen, MJ (reprint author), Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. EM mjc@ufl.edu RI Lewis, David/B-6313-2009 OI Lewis, David/0000-0002-8094-1577 FU US Environmental Protection Agency FX This paper arose from a "Geographically Isolated Wetlands Research Workshop" cohosted by the US Environmental Protection Agency Office of Research and Development and the Joseph W. Jones Ecological Research Center in Newton, GA, November 18-21, 2013. We thank the organizers. Donna Downing, Barbara Bedford, and Arnold van der Valk provided valuable comments on an early draft. Information in this document has been funded in part by the US Environmental Protection Agency. This manuscript has been subjected to agency review and has been approved for publication. The views expressed in this journal article are those of the authors and do not necessarily reflect the views or policies of the US Environmental Protection Agency. NR 142 TC 6 Z9 8 U1 26 U2 62 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 23 PY 2016 VL 113 IS 8 BP 1978 EP 1986 DI 10.1073/pnas.1512650113 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DE4SK UT WOS:000370620300032 PM 26858425 ER PT J AU Verma, S Baig, RBN Nadagouda, MN Varma, RS AF Verma, Sanny Baig, R. B. Nasir Nadagouda, Mallikarjuna N. Varma, Rajender S. TI Sustainable Strategy Utilizing Biomass: Visible-Light-Mediated Synthesis of -Valerolactone SO CHEMCATCHEM LA English DT Article DE biomass; heterogeneous catalysis; levulinic acid; sustainable chemistry; -valerolactone ID GRAPHITIC CARBON NITRIDE; C-H ACTIVATION; GAMMA-VALEROLACTONE; GRAPHENE OXIDE; LEVULINIC ACID; SULFONIC-ACID; CONVERSION; HYDROGENATION; CATALYSTS; EFFICIENT AB A novel sustainable approach to valued -valerolactone was investigated. This approach exploits the visible-light-mediated conversion of biomass-derived levulinic acid by using a bimetallic catalyst on a graphitic carbon nitride, AgPd@g-C3N4. C1 [Verma, Sanny; Baig, R. B. Nasir; Varma, Rajender S.] US EPA, Natl Risk Management Res Lab, Sustainable Technol Div, MS 443, Cincinnati, OH 45268 USA. [Nadagouda, Mallikarjuna N.] US EPA, Natl Risk Management Res Lab, WSWRD, WQMB, MS 443, Cincinnati, OH 45268 USA. RP Varma, RS (reprint author), US EPA, Natl Risk Management Res Lab, Sustainable Technol Div, MS 443, Cincinnati, OH 45268 USA. EM varma.rajender@epa.gov FU Postgraduate Research Program at the National Risk Management Research Laboratory FX S. V. and N. B. R. B. were supported by the Postgraduate Research Program at the National Risk Management Research Laboratory administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U. S. Environmental Protection Agency. NR 25 TC 6 Z9 6 U1 7 U2 24 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 1867-3880 EI 1867-3899 J9 CHEMCATCHEM JI ChemCatChem PD FEB 18 PY 2016 VL 8 IS 4 BP 690 EP 693 DI 10.1002/cctc.201501352 PG 4 WC Chemistry, Physical SC Chemistry GA DF3CZ UT WOS:000371222600003 ER PT J AU Schumacher, BA Zimmerman, JH Elliot, RJ Swanson, GR AF Schumacher, Brian A. Zimmerman, John H. Elliot, R. James Swanson, Greg R. TI The Effect of Equilibration Time and Tubing Material on Soil Gas Measurements SO SOIL & SEDIMENT CONTAMINATION LA English DT Article DE TCE; trichloroethylene; equilibration time; tubing type; volatile organic compounds; Soil gas AB The collection of soil vapor samples representative of in-situ conditions presents challenges associated with the unavoidable disturbance of the subsurface and potential losses to the atmosphere. This article evaluates the effects of two variables that influence the concentration of volatile organic compounds in soil vapor samples: equilibration time and tubing material. The time for three types of soil vapor probes (i.e., macro-purge, mini-purge, and post-run tubing probes [PRT]) to equilibrate with subsurface conditions was assessed by installing probes and collecting multiple samples over a 72-hour period. The effect of tubing material was evaluated by collocating soil vapor probes constructed with different tubing material and collecting samples over several months. We recommend that soil vapor probes constructed with a sand filter-pack and bentonite seal (i.e., macro-purge probe) equilibrate for 24 to 48 hours prior to sample collection. Post-run tubing (PRT) probes equilibrated within one to two hours while a new probe design, (i.e., mini-purge probe) equilibrated and could be sampled after only 30 minutes for screening assessments. Nylaflow, Teflon (R), polyetheretherketone (PEEK), and stainless-steel tubing had comparable trichloroethene (TCE) concentrations over all sampling time frames. We recommend that copper tubing be avoided and polyethylene only be used for screening assessments. C1 [Schumacher, Brian A.; Zimmerman, John H.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Div Environm Sci, 944 E Harmon Ave, Las Vegas, NV 89119 USA. [Elliot, R. James] Tetra Tech Inc, Santa Barbara, CA USA. [Swanson, Greg R.] Tetra Tech Inc, San Diego, CA USA. RP Schumacher, BA (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab, Div Environm Sci, 944 E Harmon Ave, Las Vegas, NV 89119 USA. EM Schumacher.brian@epa.gov RI Zimmerman, John/S-8349-2016 FU United States Environmental Protection Agency through its Office of Research and Development [EP-C-05-061] FX The United States Environmental Protection Agency through its Office of Research and Development funded, managed, and collaborated in the research described here under contract EP-C-05-061 to Tetra Tech EM Incorporated. It has been subjected to Agency review and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 11 TC 0 Z9 0 U1 4 U2 4 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1532-0383 EI 1549-7887 J9 SOIL SEDIMENT CONTAM JI Soil. Sediment. Contam. PD FEB 17 PY 2016 VL 25 IS 2 BP 151 EP 163 DI 10.1080/15320383.2016.1111860 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DJ0QW UT WOS:000373910400004 ER PT J AU Diamond, GL Bradham, KD Brattin, WJ Burgess, M Griffin, S Hawkins, CA Juhasz, AL Klotzbach, JM Nelson, C Lowney, YW Scheckel, KG Thomas, DJ AF Diamond, Gary L. Bradham, Karen D. Brattin, William J. Burgess, Michele Griffin, Susan Hawkins, Cheryl A. Juhasz, Albert L. Klotzbach, Julie M. Nelson, Clay Lowney, Yvette W. Scheckel, Kirk G. Thomas, David J. TI Predicting oral relative bioavailability of arsenic in soil from in vitro bioaccessibility SO JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES LA English DT Article ID CONTAMINATED SOILS; LEAD; VALIDATION; REGRESSION; ASSAY; CADMIUM; ERRORS; SWINE AB Several investigations have been conducted to develop in vitro bioaccessibility (IVBA) assays that reliably predict in vivo oral relative bioavailability (RBA) of arsenic (As). This study describes a meta-regression model relating soil As RBA and IVBA that is based upon data combined from previous investigations that examined the relationship between As IVBA and RBA when IVBA was determined using an extraction of soil in 0.4 M glycine at pH 1.5. Data used to develop the model included paired IVBA and RBA estimates for 83 soils from various types of sites such as mining, smelting, and pesticide or herbicide application. The following linear regression model accounted for 87% of the observed variance in RBA (R-2 = .87): RBA(%) = 0.79 x IVBA(%) + 3. This regression model is more robust than previously reported models because it includes a larger number of soil samples, and also accounts for variability in RBA and IVBA measurements made on samples collected from sites contaminated with different As sources and conducted in different labs that have utilized different experimental models for estimating RBA. C1 [Diamond, Gary L.; Brattin, William J.; Klotzbach, Julie M.] SRC Inc, North Syracuse, NY USA. [Bradham, Karen D.; Nelson, Clay] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Burgess, Michele; Hawkins, Cheryl A.] US EPA, Off Superfund Remediat & Technol Innovat, Sci Policy Branch, Washington, DC 20460 USA. [Griffin, Susan] US EPA, Denver, CO USA. [Juhasz, Albert L.] Univ S Australia, Ctr Environm Risk Assessment & Remediat, Adelaide, SA 5001, Australia. [Lowney, Yvette W.] Exponent Inc, Boulder, CO USA. [Scheckel, Kirk G.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Thomas, David J.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. RP Diamond, GL (reprint author), SRC Inc, 8191 Cedar St, Akron, NY 14001 USA. EM diamond@srcinc.com RI Juhasz, Albert/F-6600-2011; OI Juhasz, Albert/0000-0002-1164-4085; Scheckel, Kirk/0000-0001-9326-9241 FU U.S. Environmental Protection Agency FX The U.S. Environmental Protection Agency funded and managed the research described here. It has been subjected to agency review and approved for publication. No attempt was made to validate data in the cited literature. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 31 TC 1 Z9 1 U1 7 U2 21 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1528-7394 EI 1087-2620 J9 J TOXICOL ENV HEAL A JI J. Toxicol. Env. Health Part A PD FEB 16 PY 2016 VL 79 IS 4 BP 165 EP 173 DI 10.1080/15287394.2015.1134038 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DI8XE UT WOS:000373784900003 PM 27029599 ER PT J AU Angrish, MM Pleil, JD Stiegel, MA Madden, MC Moser, VC Herr, DW AF Angrish, Michelle M. Pleil, Joachim D. Stiegel, Matthew A. Madden, Michael C. Moser, Virginia C. Herr, David W. TI Taxonomic applicability of inflammatory cytokines in adverse outcome pathway (AOP) development SO JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES LA English DT Article ID IN-VITRO; RISK-ASSESSMENT; EMERGING TECHNOLOGIES; BIOMONITORING DATA; SAFETY ASSESSMENT; DISEASE; CHEMOKINES; BIOMARKERS; MECHANISMS; TOXICITY AB Cytokines, low-molecular-weight messenger proteins that act as intercellular immunomodulatory signals, have become a mainstream preclinical marker for assessing the systemic inflammatory response to external stressors. The challenge is to quantitate from healthy subjects cytokine levels that are below or at baseline and relate those dynamic and complex cytokine signatures of exposures with the inflammatory and repair pathways. Thus, highly sensitive, specific, and precise analytical and statistical methods are critically important. Investigators at the U.S. Environmental Protection Agency (EPA) have implemented advanced technologies and developed statistics for evaluating panels of inflammatory cytokines in human blood, exhaled breath condensate, urine samples, and murine biological media. Advanced multiplex, bead-based, and automated analytical platforms provided sufficient sensitivity, precision, and accuracy over the traditional enzyme-linked immunosorbent assay (ELISA). Thus, baseline cytokine levels can be quantified from healthy human subjects and animals and compared to an in vivo exposure response from an environmental chemical. Specifically, patterns of cytokine responses in humans exposed to environmental levels of ozone and diesel exhaust, and in rodents exposed to selected pesticides (such as fipronil and carbaryl), were used as case studies to generally assess the taxonomic applicability of cytokine responses. The findings in this study may aid in the application of measureable cytokine markers in future adverse outcome pathway (AOP)-based toxicity testing. Data from human and animal studies were coalesced and the possibility of using cytokines as key events (KE) to bridge species responses to external stressors in an AOP-based framework was explored. C1 [Angrish, Michelle M.] US EPA, Integrated Sci & Toxicol Div, NHEERL ORD, Res Triangle Pk, NC 27711 USA. [Pleil, Joachim D.] US EPA, Human Exposure & Atmospher Sci Div, NERL ORD, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Stiegel, Matthew A.] US EPA, ORISE, Res Triangle Pk, NC 27711 USA. [Madden, Michael C.] US EPA, Environm Publ Hlth Div, NHEERL ORD, Chapel Hill, NC USA. [Moser, Virginia C.] US EPA, Neurotoxicol Branch, Tox Assessment Div NHEERL ORD, Res Triangle Pk, NC 27711 USA. [Herr, David W.] US EPA, Tox Assessment Div, NHEERL ORD, Res Triangle Pk, NC USA. RP Pleil, JD (reprint author), US EPA, Human Exposure & Atmospher Sci Div, NERL ORD, 109 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM pleil@unc.edu NR 62 TC 2 Z9 2 U1 0 U2 3 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1528-7394 EI 1087-2620 J9 J TOXICOL ENV HEAL A JI J. Toxicol. Env. Health Part A PD FEB 16 PY 2016 VL 79 IS 4 BP 184 EP 196 DI 10.1080/15287394.2016.1138923 PG 13 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DI8XE UT WOS:000373784900005 PM 26914248 ER PT J AU Fan, DM Bradley, MJ Hinkle, AW Johnson, RL Tratnyek, PG AF Fan, Dimin Bradley, Miranda J. Hinkle, Adrian W. Johnson, Richard L. Tratnyek, Paul G. TI Chemical Reactivity Probes for Assessing Abiotic Natural Attenuation by Reducing Iron Minerals SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID ZERO-VALENT IRON; ELECTRON-TRANSFER MEDIATORS; FREE-ENERGY RELATIONSHIPS; BEARING SOIL MINERALS; OXIDE-WATER INTERFACE; REDUCTIVE DECHLORINATION; CIS-DICHLOROETHYLENE; CARBON-TETRACHLORIDE; FE-II; ORGANIC-MATTER AB Increasing recognition that abiotic natural attenuation (NA) of chlorinated solvents can be important has created demand for improved methods to characterize the redox properties of the aquifer materials that are responsible for abiotic NA. This study explores one promising approach: using chemical reactivity probes (CRPs) to characterize the thermodynamic and kinetic aspects of contaminant reduction by reducing iron minerals. Assays of thermodynamic CRPs were developed to determine the reduction potentials (E-CRP) of suspended minerals by spectrophotometric determination of equilibrium CRP speciation and calculations using the Nernst equation. E-CRP varied as expected with mineral type, mineral loading, and Fe(II) concentration. Comparison of E-CRP with reduction potentials measured potentiometrically using a Pt electrode (E-Pt) showed that E-CRP was 100-150 mV more negative than E-Pt. When E-Pt was measured with small additions of CRPs, the systematic difference between E-Pt and E-CRP was eliminated, suggesting that these CRPs are effective mediators of electron transfer between mineral and electrode surfaces. Model contaminants (4-chloronitrobenzene, 2-chloroacetophenone, and carbon tetrachloride) were used as kinetic CRPs. The reduction rate constants of kinetic CRPs correlated well with the E-CRP for mineral suspensions. Using the rate constants compiled from literature for contaminants and relative mineral reduction potentials based on E-CRP measurements, qualitatively consistent trends were obtained, suggesting that CRP-based assays may be useful for estimating abiotic NA rates of contaminants in groundwater. C1 [Fan, Dimin; Bradley, Miranda J.; Hinkle, Adrian W.; Johnson, Richard L.; Tratnyek, Paul G.] Oregon Hlth & Sci Univ, Inst Environm Hlth, 3181 SW Sam Jackson Pk Rd, Portland, OR 97239 USA. [Fan, Dimin] US EPA, Off Superfund Remediat & Technol Innovat, Arlington, VA 22202 USA. RP Tratnyek, PG (reprint author), Oregon Hlth & Sci Univ, Inst Environm Hlth, 3181 SW Sam Jackson Pk Rd, Portland, OR 97239 USA. EM tratnyek@ohsu.edu RI fan, dimin/D-3200-2017 FU Strategic Environmental Research and Development Program of the U.S. Department of Defense [ER-2308]; NSF Center for Coastal Margin Observation and Research FX The work was supported by a grant from the Strategic Environmental Research and Development Program of the U.S. Department of Defense (ER-2308). Surface areas of the oxides were measured by Maria Fuentes-Deonate, while on an internship funded by the NSF Center for Coastal Margin Observation and Research. NR 59 TC 2 Z9 2 U1 17 U2 37 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD FEB 16 PY 2016 VL 50 IS 4 BP 1868 EP 1876 DI 10.1021/acs.est.5b05800 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DE2JS UT WOS:000370454200028 PM 26814150 ER PT J AU Oliver, DM Porter, KDH Pachepsky, YA Muirhead, RW Reaney, SM Coffey, R Kay, D Milledge, DG Hong, E Anthony, SG Page, T Bloodworth, JW Mellander, PE Carbonneau, PE McGrane, SJ Quilliam, RS AF Oliver, David M. Porter, Kenneth D. H. Pachepsky, Yakov A. Muirhead, Richard W. Reaney, Sim M. Coffey, Rory Kay, David Milledge, David G. Hong, Eunmi Anthony, Steven G. Page, Trevor Bloodworth, Jack W. Mellander, Per-Erik Carbonneau, Patrice E. McGrane, Scott J. Quilliam, Richard S. TI Predicting microbial water quality with models: Over-arching questions for managing risk in agricultural catchments SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article ID FECAL INDICATOR BACTERIA; ESCHERICHIA-COLI; LAND-USE; SIMULATED RAINFALL; BORNE PATHOGENS; SURFACE WATERS; CLIMATE-CHANGE; HEALTH-RISKS; ANIMAL WASTE; FLOOD RISK AB The application of models to predict concentrations of faecal indicator organisms (FIOs) in environmental systems plays an important role for guiding decision-making associated with the management of microbial water quality. In recent years there has been an increasing demand by policy-makers for models to help inform FIO dynamics in order to prioritise efforts for environmental and human-health protection. However, given the limited evidence-base on which FIO models are built relative to other agricultural pollutants (e.g. nutrients) it is imperative that the end-user expectations of FIO models are appropriately managed. In response, this commentary highlights four over-arching questions associated with: (i) model purpose; (ii) modelling approach; (iii) data availability; and (iv) model application, that must be considered as part of good practice prior to the deployment of any modelling approach to predict FIO behaviour in catchment systems. A series of short and longer-term research priorities are proposed in response to these questions in order to promote better model deployment in the field of catchment microbial dynamics. (C) 2015 The Authors. Published by Elsevier B.V. C1 [Oliver, David M.; Porter, Kenneth D. H.; Quilliam, Richard S.] Univ Stirling, Sch Nat Sci, Biol & Environm Sci, Stirling FK9 4LA, Scotland. [Pachepsky, Yakov A.; Hong, Eunmi] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Muirhead, Richard W.] AgResearch, Land & Environm, Invermay Res Ctr, Private Bag 50034, Mosgiel 9053, New Zealand. [Reaney, Sim M.; Milledge, David G.; Carbonneau, Patrice E.] Univ Durham, Dept Geog, Durham DH1 3LE, England. [Coffey, Rory] Univ Coll Dublin, Agr & Food Sci Ctr, Sch Biosyst Engn, Dublin 2, Ireland. [Kay, David] Aberystwyth Univ, Ctr Res Environm & Hlth, Aberystwyth SY23 3DB, Dyfed, Wales. [Anthony, Steven G.] ADAS Grp Ltd, HQ Pendeford House,Pendeford Business Pk, Wolverhampton WV9 5AP, W Midlands, England. [Page, Trevor] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Bloodworth, Jack W.] Cranfield Univ, Cranfield Water Sci Inst, Cranfield MK43 0AL, Beds, England. [Mellander, Per-Erik] TEAGASC, Agr Catchments Programme, Johnstown Castle, Wexford, Ireland. [McGrane, Scott J.] Univ Surrey, Dept Civil & Environm Engn, Surrey GU2 7XH, England. [Coffey, Rory] US EPA, Off Res & Dev, Washington, DC 20460 USA. RP Oliver, DM (reprint author), Univ Stirling, Sch Nat Sci, Biol & Environm Sci, Stirling FK9 4LA, Scotland. EM david.oliver@stir.ac.uk RI Coffey, Rory/G-4542-2014; OI Coffey, Rory/0000-0001-5941-0205; Quilliam, Richard/0000-0001-7020-4410; Oliver, David/0000-0002-6200-562X; Milledge, David/0000-0003-4077-4898; McGrane, Scott/0000-0002-5277-1347; Pachepsky, Yakov/0000-0003-0232-6090; Muirhead, Richard/0000-0002-0913-561X FU UK Natural Environment Research Council, 'PRACTICAL Modelling' project [NE/M005860/1] FX This work was supported by the UK Natural Environment Research Council, as part of the 'PRACTICAL Modelling' project (NE/M005860/1). We are grateful for the constructive comments of the three reviewers and the handling editor. NR 74 TC 6 Z9 6 U1 1 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 15 PY 2016 VL 544 BP 39 EP 47 DI 10.1016/j.scitotenv.2015.11.086 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DC8TB UT WOS:000369491500006 PM 26657248 ER PT J AU Brack, W Ait-Aissa, S Burgess, RM Busch, W Creusot, N Di Paolo, C Escher, BI Hewitt, LM Hilscherova, K Hollender, J Hollert, H Jonker, W Kool, J Lamoree, M Muschket, M Neumann, S Rostkowski, P Ruttkies, C Schollee, J Schymanski, EL Schulze, T Seiler, TB Tindall, AJ Umbuzeiro, GD Vrana, B Krauss, M AF Brack, Werner Ait-Aissa, Selim Burgess, Robert M. Busch, Wibke Creusot, Nicolas Di Paolo, Carolina Escher, Beate I. Hewitt, L. Mark Hilscherova, Klara Hollender, Juliane Hollert, Henner Jonker, Willem Kool, Jeroen Lamoree, Marja Muschket, Matthias Neumann, Steffen Rostkowski, Pawel Ruttkies, Christoph Schollee, Jennifer Schymanski, Emma L. Schulze, Tobias Seiler, Thomas-Benjamin Tindall, Andrew J. Umbuzeiro, Gisela De Aragao Vrana, Branislav Krauss, Martin TI Effect-directed analysis supporting monitoring of aquatic environments - An in-depth overview SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Review DE Toxicant identification; Bioassay; Dosing; Enrichment; Fractionation; Non-target analysis ID 2-DIMENSIONAL LIQUID-CHROMATOGRAPHY; RESOLUTION MASS-SPECTROMETRY; POLYCYCLIC AROMATIC-HYDROCARBONS; SOLVATION ENERGY RELATIONSHIPS; SUSPENDED PARTICULATE MATTER; REPORTER GENE ASSAY; SUPERCRITICAL-FLUID CHROMATOGRAPHY; CAPILLARY GAS-CHROMATOGRAPHY; RECEPTOR-MEDIATED ACTIVITIES; PRINCIPAL COMPONENT ANALYSIS AB Aquatic environments are often contaminated with complex mixtures of chemicals that may pose a risk to ecosystems and human health. This contamination cannot be addressed with target analysis alone but tools are required to reduce this complexity and identify those chemicals that might cause adverse effects. Effect-directed analysis (EDA) is designed to meet this challenge and faces increasing interest in water and sediment quality monitoring. Thus, the present paper summarizes current experience with the EDA approach and the tools required, and provides practical advice on their application. The paper highlights the need for proper problem formulation and gives general advice for study design. As the EDA approach is directed by toxicity, basic principles for the selection of bioassays are given as well as a comprehensive compilation of appropriate assays, including their strengths and weaknesses. A specific focus is given to strategies for sampling, extraction and bioassay dosing since they strongly impact prioritization of toxicants in EDA. Reduction of sample complexity mainly relies on fractionation procedures, which are discussed in this paper, including quality assurance and quality control. Automated combinations of fractionation, biotesting and chemical analysis using so-called hyphenated tools can enhance the throughput and might reduce the risk of artifacts in laboratory work. The key to determining the chemical structures causing effects is analytical toxicant identification. The latest approaches, tools, software and databases for target-, suspect and non-target screening as well as unknown identification are discussed together with analytical and toxicological confirmation approaches. A better understanding of optimal use and combination of EDA tools will help to design efficient and successful toxicant identification studies in the context of quality monitoring in multiply stressed environments. (C) 2015 Elsevier B.V. All rights reserved. C1 [Brack, Werner; Busch, Wibke; Escher, Beate I.; Muschket, Matthias; Schulze, Tobias; Krauss, Martin] UFZ Helmholtz Ctr Environm Res, Permoserstr 15, D-04318 Leipzig, Germany. [Brack, Werner; Di Paolo, Carolina; Hollert, Henner; Seiler, Thomas-Benjamin] Rhein Westfal TH Aachen, Worringerweg 1, D-52074 Aachen, Germany. [Ait-Aissa, Selim; Creusot, Nicolas] Inst Natl Environm Ind & Risques INERIS, BP2, F-60550 Verneuil En Halatte, France. [Burgess, Robert M.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Atlantic Ecol Div, Narragansett, RI USA. [Escher, Beate I.] Univ Tubingen, D-72074 Tubingen, Germany. [Hewitt, L. Mark] Environm Canada, Water Sci & Technol Directorate, 867 Lakeshore Rd, Burlington, ON L7S 1A1, Canada. [Hilscherova, Klara; Vrana, Branislav] Masaryk Univ, Res Ctr Tox Cpds Environm RECETOX, Kamenice 753-5, Brno 62500, Czech Republic. [Hollender, Juliane; Schollee, Jennifer; Schymanski, Emma L.] Swiss Fed Inst Aquat Sci & Technol, Eawag, CH-8600 Dubendorf, Switzerland. [Jonker, Willem; Kool, Jeroen] Vrije Univ Amsterdam, BioMol Anal Grp, Amsterdam, Netherlands. [Lamoree, Marja] Vrije Univ Amsterdam, Inst Environm Studies, Amsterdam, Netherlands. [Neumann, Steffen; Ruttkies, Christoph] Leibniz Inst Plant Biochem, Halle, Saale, Germany. [Rostkowski, Pawel] NILU Norwegian Inst Air Res, Inst Veien 18, N-2007 Kjeller, Norway. [Tindall, Andrew J.] WatchFrag, Batiment Genavenir 3,1 Rue Pierre Fontaine, F-91000 Evry, France. [Umbuzeiro, Gisela De Aragao] Univ Estadual Campinas, Limeira, Brazil. RP Brack, W (reprint author), UFZ Helmholtz Ctr Environm Res, Permoserstr 15, D-04318 Leipzig, Germany. RI Umbuzeiro, Gisela/H-4603-2011; Lamoree, Marja/N-4396-2013; Krauss, Martin/E-1672-2012; Schulze, Tobias/B-1553-2010; Escher, Beate/C-7992-2009; Seiler, Thomas-Benjamin/K-6294-2012; Hollender, Juliane/A-2145-2014; OI Umbuzeiro, Gisela/0000-0002-8623-5200; Lamoree, Marja/0000-0002-7373-7738; Krauss, Martin/0000-0002-0362-4244; Schulze, Tobias/0000-0002-9744-8914; Escher, Beate/0000-0002-5304-706X; Tindall, Andrew/0000-0001-8119-0923; Di Paolo, Carolina/0000-0002-4281-1034; Seiler, Thomas-Benjamin/0000-0001-8127-510X; Hollender, Juliane/0000-0002-4660-274X; Brack, Werner/0000-0001-9269-6524; Ruttkies, Christoph/0000-0002-8621-8689 FU project FP7 SOLUTIONS under the European Union Seventh Framework Program [603437]; project FP7 EDA-EMERGE under the European Union Seventh Framework Program [PITN-GA-2011-290100] FX This paper has been prepared as a deliverable of the NORMAN network on emerging pollutants and supported by the projects FP7 SOLUTIONS (grant agreement no. 603437) and FP7 EDA-EMERGE (Contract Number PITN-GA-2011-290100) under the European Union Seventh Framework Program. NR 461 TC 20 Z9 20 U1 41 U2 122 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 15 PY 2016 VL 544 BP 1073 EP 1118 DI 10.1016/j.scitotenv.2015.11.102 PG 46 WC Environmental Sciences SC Environmental Sciences & Ecology GA DC8TB UT WOS:000369491500112 PM 26779957 ER PT J AU Krueger, WS Wade, TJ AF Krueger, Whitney S. Wade, Timothy J. TI Elevated blood lead and cadmium levels associated with chronic infections among non-smokers in a cross-sectional analysis of NHANES data SO ENVIRONMENTAL HEALTH LA English DT Article DE Helicobacter pylori; Toxoplasma; Hepatitis B; Nutrition surveys; Seroprevalence; Heavy metals; Immune system; Immunomodulation ID HELICOBACTER-PYLORI INFECTION; UNITED-STATES; IMMUNE-SYSTEM; HEAVY-METALS; EXPOSURE; MICE; IMMUNOTOXICITY; HEPATITIS; HEALTH; VIRUS AB Background: Experimental animal studies, in vitro experiments, and clinical assessments have shown that metal toxicity can impair immune responses. We analyzed data from a United States representative National Health and Nutrition Examination Survey (NHANES) to explore associations between chronic infections and elevated blood concentrations of lead and cadmium among non-smoking NHANES participants. Methods: NHANES data from 1999 to 2012 were examined and weighted to represent the United States population. Multivariable logistic regression was used to estimate adjusted odds ratios (AOR) and 95 % confidence intervals (CI) for heavy metal associations with seropositivity for Helicobacter pylori, Toxoplasma gondii, and Hepatitis B virus (HBV) infections. Results: Available 2-year survey cycles for infection seroprevalence varied by pathogen, from 1 to 7 cycles. Available sample size, disease seroprevalence, and participant age range also varied by pathogen of interest. After controlling for demographic characteristics and general health condition, an elevated blood lead level above the survey population median was significantly associated with seropositivity for all three pathogens (AORs = 1.2-1.5). In addition, an elevated blood cadmium level above the median was significantly associated with HBV (AOR = 1.5; 95 % CI = 1.2-2.0) and H. pylori (AOR = 1.5; 95 % CI = 1.2-1.7) seropositivity. Age-specific analyses for H. pylori and T. gondii indicated stronger associations among children under 13 years of age, particularly for lead exposure and H. pylori seropositivity, and weaker associations among those over 35 years of age. Conclusions: The results of this cross-sectional human health survey suggest that the immunological effects of lead and cadmium toxicity may be associated with an increased susceptibility to chronic infections. C1 [Krueger, Whitney S.] RTI Hlth Solut, 3040 Cornwallis Rd,POB 12194, Res Triangle Pk, NC 27709 USA. [Krueger, Whitney S.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Wade, Timothy J.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Environm Publ Hlth Div, Chapel Hill, NC 27514 USA. RP Wade, TJ (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Environm Publ Hlth Div, Chapel Hill, NC 27514 USA. EM wade.tim@epa.gov NR 62 TC 1 Z9 1 U1 4 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1476-069X J9 ENVIRON HEALTH-GLOB JI Environ. Health PD FEB 11 PY 2016 VL 15 AR 16 DI 10.1186/s12940-016-0113-4 PG 13 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DE2TC UT WOS:000370478900001 PM 26864738 ER PT J AU Hartz, AMS Zhong, Y Wolf, A LeVine, H Miller, DS Bauer, B AF Hartz, Anika M. S. Zhong, Yu Wolf, Andrea LeVine, Harry, III Miller, David S. Bauer, Bjoern TI A beta 40 Reduces P-Glycoprotein at the Blood-Brain Barrier through the Ubiquitin-Proteasome Pathway SO JOURNAL OF NEUROSCIENCE LA English DT Article DE Alzheimer's disease; blood-brain barrier; P-glycoprotein; transporter; ubiquitin-proteasome system ID CEREBRAL AMYLOID ANGIOPATHY; ALZHEIMERS-DISEASE; MOUSE MODEL; A-BETA; UP-REGULATION; CELL-SURFACE; CANCER-CELLS; RESISTANCE; MECHANISMS; EXPRESSION AB Failure to clear amyloid-beta (A beta) from the brain is in part responsible for A beta brain accumulation in Alzheimer's disease (AD). A critical protein for clearing A beta across the blood-brain barrier is the efflux transporter P-glycoprotein (P-gp) in the luminal plasma membrane of the brain capillary endothelium. P-gp is reduced at the blood-brain barrier in AD, which has been shown to be associated with A beta brain accumulation. However, the mechanism responsible for P-gp reduction in AD is not well understood. Here we focused on identifying critical mechanistic steps involved in reducing P-gp in AD. We exposed isolated rat brain capillaries to 100 nM A beta 40, A beta 40, aggregated A beta 40, and A beta 42. We observed that only A beta 40 triggered reduction of P-gp protein expression and transport activity levels; this occurred in a dose-and time-dependent manner. To identify the steps involved in A beta-mediated P-gp reduction, we inhibited protein ubiquitination, protein trafficking, and the ubiquitin-proteasome system, and monitored P-gp protein expression, transport activity, and P-gp-ubiquitin levels. Thus, exposing brain capillaries to A beta 40 triggers ubiquitination, internalization, and proteasomal degradation of P-gp. These findings may provide potential therapeutic targets within the blood-brain barrier to limit P-gp degradation in AD and improve A beta brain clearance. C1 [Hartz, Anika M. S.; Zhong, Yu; LeVine, Harry, III] Univ Kentucky, Sanders Brown Ctr Aging, Lexington, KY 40536 USA. [Hartz, Anika M. S.] Univ Kentucky, Dept Pharmacol & Nutr Sci, Lexington, KY 40536 USA. [Hartz, Anika M. S.; Wolf, Andrea; Bauer, Bjoern] Univ Minnesota, Dept Pharm Practice & Pharmaceut Sci, Coll Pharm, Duluth, MN 55812 USA. [Miller, David S.] Natl Inst Environm Hlth Sci, Lab Signal Transduct, NIH, Res Triangle Pk, NC 27709 USA. [Bauer, Bjoern] Univ Kentucky, Dept Pharmaceut Sci, Coll Pharm, Lexington, KY 40536 USA. RP Hartz, AMS (reprint author), Univ Kentucky, Dept Pharmacol & Nutr Sci, Sanders Brown Ctr Aging 329, 800 South Limestone, Lexington, KY 40536 USA. EM anika.hartz@uky.edu FU National Institute on Aging Grant [1R01AG039621]; 3M Science and Technology Doctoral Fellowship; 3M Grant [P01-AG005119-20]; Division of Intramural Research at National Institute of Environmental Health Sciences/National Institutes of Health FX This work was supported by National Institute on Aging Grant 1R01AG039621 to A.M.S.H., 3M Science and Technology Doctoral Fellowship to A.W., 3M Grant P01-AG005119-20 to H.L., and the Division of Intramural Research at National Institute of Environmental Health Sciences/National Institutes of Health to D.S.M. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute on Aging or the National Institutes of Health. We thank Stephanie Edelmann, Ralf Rempe, Yu Zhong, and Tal Ashkenazi-Frolinger for proofreading the manuscript and editorial assistance; Britt Johnson, Kevin Viken, and Emma Soldner for technical assistance; and Lynne Bemis and Evan Odean for the NanoSight analysis. NR 44 TC 9 Z9 9 U1 5 U2 12 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD FEB 10 PY 2016 VL 36 IS 6 BP 1930 EP 1941 DI 10.1523/JNEUROSCI.0350-15.2016 PG 12 WC Neurosciences SC Neurosciences & Neurology GA DD5LF UT WOS:000369964500013 PM 26865616 ER PT J AU Chen, HL Marder, K AF Chen, Honglei Marder, Karen TI Milk consumption and the risk of nigral degeneration SO NEUROLOGY LA English DT Editorial Material ID PARKINSONS-DISEASE; DAIRY-PRODUCTS AB In the era of genetic research for neurodegenerative diseases, less attention has been paid to epidemiologists' search for potential environmental risk factors for Parkinson disease (PD). Epidemiologic evidence suggests that cigarette smoking is associated with about 50% lower risk of PD1 and exposure to certain pesticides such as rotenone and paraquat is associated with doubled risk.(2) Recent studies also suggest that higher concentration of serum urate, an endogenous antioxidant, is associated with a lower risk of PD.(3) Compared with these observations, another epidemiologic finding has been largely neglected. Several prospective studies,(4-7) including the Honolulu-Asia Aging Study (HAAS),(6) have reported that higher consumption of dairy products, or milk alone, was associated with higher risk for PD. C1 [Chen, Honglei] Natl Inst Environm Hlth Sci, Epidemiol Branch, Res Triangle Pk, NC USA. [Marder, Karen] Columbia Univ, Coll Phys & Surg, Dept Neurol, Taub Inst Res Alzheimers Dis & Aging Brain, New York, NY USA. RP Chen, HL (reprint author), Natl Inst Environm Hlth Sci, Epidemiol Branch, Res Triangle Pk, NC USA. EM chenh2@niehs.nih.gov OI Chen, Honglei/0000-0003-3446-7779 FU Intramural NIH HHS; NCRR NIH HHS [NIH 1UL1 RR024156-01]; NINDS NIH HHS [U01NS052592] NR 10 TC 0 Z9 0 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0028-3878 EI 1526-632X J9 NEUROLOGY JI Neurology PD FEB 9 PY 2016 VL 86 IS 6 BP 496 EP 497 DI 10.1212/WNL.0000000000002268 PG 2 WC Clinical Neurology SC Neurosciences & Neurology GA DC9KT UT WOS:000369541200002 PM 26658908 ER PT J AU Brown, CA Sharp, D Collura, TCM AF Brown, Cheryl A. Sharp, Darrin Collura, T. Chris Mochon TI Effect of climate change on water temperature and attainment of water temperature criteria in the Yaquina Estuary, Oregon (USA) SO ESTUARINE COASTAL AND SHELF SCIENCE LA English DT Article DE Climate change; Estuary; Water temperature; Modeling; Yaquina Estuary; Water quality ID ADULT CHINOOK SALMON; PACIFIC-NORTHWEST; COASTAL OCEAN; STREAM TEMPERATURES; UNITED-STATES; RIVER ESTUARY; HUDSON RIVER; NEW-YORK; VARIABILITY; SENSITIVITY AB There is increasing evidence that our planet is warming and this warming is also resulting in rising sea levels. Estuaries which are located at the interface between land and ocean are impacted by these changes. We used CE-QUAL-W2 water quality model to predict changes in water temperature as a function of increasing air temperatures and rising sea level for the Yaquina Estuary, Oregon (USA). Annual average air temperature in the Yaquina watershed is expected to increase about 0.3 degrees C per decade by 2040-2069. An air temperature increase of 3 degrees C in the Yaquina watershed is likely to result in estuarine water temperature increasing by 0.7-1.6 degrees C. Largest water temperature increases are expected in the upper portion of the estuary, while sea level rise may mitigate some of the warming in the lower portion of the estuary. Smallest changes in water temperature are predicted to occur in the summer, and maximum changes during the winter and spring. Increases in air temperature may result in an increase in the number of days per year that the 7-day maximum average temperature exceeds 18 degrees C (criterion for protection of rearing and migration of salmonids and trout) as well as other water quality concerns. In the upstream portion of the estuary, a 4 degrees C increase in air temperature is predicted to cause an increase of 40 days not meeting the temperature criterion, while in the lower estuary the increase will depend upon rate of sea level rise (ranging from 31 to 19 days). Published by Elsevier Ltd. C1 [Brown, Cheryl A.; Collura, T. Chris Mochon] US EPA, Western Ecol Div, Natl Hlth & Environm Effects Res Lab, Twinsburg, OH USA. [Sharp, Darrin] Oregon State Univ, Oregon Climate Change Res Inst, Corvallis, OR 97331 USA. RP Brown, CA (reprint author), US EPA, Western Ecol Div, Natl Hlth & Environm Effects Res Lab, Twinsburg, OH USA. EM brown.cheryl@epa.gov FU USEPA; USGS FX We would like to acknowledge Deborah Reusser (USGS) for funding Oregon Climate Research Institute, Pat Clinton (US EPA) for GIS support and Anne Sigleo (US EPA, retired) for providing water temperature data from Elk City. Dr. Rochelle Labiosa (US EPA, Region 10) and Dr. Heejun Chang (Portland State University) kindly reviewed previous versions of this manuscript. The information in this document has been funded by the USEPA and USGS. It has been subjected to review by the National Health and Environmental Effects Research Laboratory's Western Ecology Division and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. NR 57 TC 0 Z9 0 U1 10 U2 23 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0272-7714 EI 1096-0015 J9 ESTUAR COAST SHELF S JI Estuar. Coast. Shelf Sci. PD FEB 5 PY 2016 VL 169 BP 136 EP 146 DI 10.1016/j.ecss.2015.11.006 PG 11 WC Marine & Freshwater Biology; Oceanography SC Marine & Freshwater Biology; Oceanography GA DE8KJ UT WOS:000370884600014 ER PT J AU Ruiz, JD Quackenboss, JJ Tulve, NS AF Ruiz, Jazmin Del Carmen Quackenboss, James J. Tulve, Nicolle S. TI Contributions of a Child's Built, Natural, and Social Environments to Their General Cognitive Ability: A Systematic Scoping Review SO PLOS ONE LA English DT Review ID LOW-BIRTH-WEIGHT; 1ST 3 YEARS; POLYUNSATURATED FATTY-ACIDS; PRENATAL COCAINE EXPOSURE; BLOOD LEAD CONCENTRATIONS; SCHOOL-AGE-CHILDREN; INFANT MENTAL-DEVELOPMENT; INNER-CITY CHILDREN; IN-UTERO EXPOSURE; POLYCYCLIC AROMATIC-HYDROCARBONS AB The etiology of a child's cognitive ability is complex, with research suggesting that it is not attributed to a single determinant or even a defined period of exposure. Rather, cognitive development is the product of cumulative interactions with the environment, both negative and positive, over the life course. The aim of this systematic scoping review was to collate evidence associated with children's cognitive health, including inherent factors as well as chemical and non-chemical stressors from the built, natural, and social environments. Three databases were used to identify recent epidemiological studies (2003-2013) that examined exposure factors associated with general cognitive ability in children. Over 100 factors were evaluated from 258 eligible studies. We found that recent literature mainly assessed the hypothesized negative effects of either inherent factors or chemical exposures present in the physical environment. Prenatal growth, sleep health, lead and water pollutants showed consistent negative effects. Of the few studies that examined social stressors, results consistently showed cognitive development to be influenced by both positive and negative social interactions at home, in school or the community. Among behavioral factors related to diet and lifestyle choices of the mother, breastfeeding was the most studied, showing consistent positive associations with cognitive ability. There were mostly inconsistent results for both chemical and non-chemical stressors. The majority of studies utilized traditional exposure assessments, evaluating chemical and non-chemical stressors separately. Collective evidence from a limited number of studies revealed that cumulative exposure assessment that incorporates multiple chemical and non-chemical stressors over the life course may unravel the variability in effect on cognitive development and help explain the inconsistencies across studies. Future research examining the interactions of multiple stressors within a child's total environment, depicting a more real-world exposure, will aid in understanding the cumulative effects associated with a child's ability to learn. C1 [Ruiz, Jazmin Del Carmen] Oak Ridge Inst Sci & Educ, Res Participat Program, Oak Ridge, TN USA. [Ruiz, Jazmin Del Carmen; Tulve, Nicolle S.] US EPA, Human Exposure & Atmospher Sci Div, Natl Exposure Res Lab, Off Res & Dev, Durham, NC USA. [Quackenboss, James J.] US EPA, Human Exposure & Atmospher Sci Div, Natl Exposure Res Lab, Off Res & Dev, Las Vegas, NV 89193 USA. RP Ruiz, JD (reprint author), Oak Ridge Inst Sci & Educ, Res Participat Program, Oak Ridge, TN USA.; Ruiz, JD (reprint author), US EPA, Human Exposure & Atmospher Sci Div, Natl Exposure Res Lab, Off Res & Dev, Durham, NC USA. EM jaz13ruiz@gmail.com FU U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, Human Exposure and Atmospheric Sciences Division, Exposure Measurements and Analysis Branch FX This project was supported by an appointment to the Internship/Research Participation Program at the U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, Human Exposure and Atmospheric Sciences Division, Exposure Measurements and Analysis Branch, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy and EPA. It has been subjected to Agency administrative review and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 294 TC 1 Z9 1 U1 14 U2 25 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 3 PY 2016 VL 11 IS 2 AR e0147741 DI 10.1371/journal.pone.0147741 PG 44 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9OF UT WOS:000369550200057 ER PT J AU Lu, J Goldsmith, MR Grulke, CM Chang, DT Brooks, RD Leonard, JA Phillips, MB Hypes, ED Fair, MJ Tornero-Velez, R Johnson, J Dary, CC Tan, YM AF Lu, Jingtao Goldsmith, Michael-Rock Grulke, Christopher M. Chang, Daniel T. Brooks, Raina D. Leonard, Jeremy A. Phillips, Martin B. Hypes, Ethan D. Fair, Matthew J. Tornero-Velez, Rogelio Johnson, Jeffre Dary, Curtis C. Tan, Yu-Mei TI Developing a Physiologically-Based Pharmacokinetic Model Knowledgebase in Support of Provisional Model Construction SO PLOS COMPUTATIONAL BIOLOGY LA English DT Article ID PLASMA PARTITION-COEFFICIENTS; ROUTE DEPENDENT DOSIMETRY; HUMAN LIVER-MICROSOMES; SPRAGUE-DAWLEY RATS; QUANTITATIVE STRUCTURE; PHARMACOKINETIC/PHARMACODYNAMIC MODEL; CHEMICAL SIMILARITY; RISK-ASSESSMENT; IN-VIVO; POLYCHLORINATED-BIPHENYLS AB Developing physiologically-based pharmacokinetic (PBPK) models for chemicals can be resource-intensive, as neither chemical-specific parameters nor in vivo pharmacokinetic data are easily available for model construction. Previously developed, well-parameterized, and thoroughly-vetted models can be a great resource for the construction of models pertaining to new chemicals. A PBPK knowledgebase was compiled and developed from existing PBPK-related articles and used to develop new models. From 2,039 PBPK-related articles published between 1977 and 2013, 307 unique chemicals were identified for use as the basis of our knowledgebase. Keywords related to species, gender, developmental stages, and organs were analyzed from the articles within the PBPK knowledgebase. A correlation matrix of the 307 chemicals in the PBPK knowledgebase was calculated based on pharmacokinetic-relevant molecular descriptors. Chemicals in the PBPK knowledgebase were ranked based on their correlation toward ethylbenzene and gefitinib. Next, multiple chemicals were selected to represent exact matches, close analogues, or non-analogues of the target case study chemicals. Parameters, equations, or experimental data relevant to existing models for these chemicals and their analogues were used to construct new models, and model predictions were compared to observed values. This compiled knowledgebase provides a chemical structure-based approach for identifying PBPK models relevant to other chemical entities. Using suitable correlation metrics, we demonstrated that models of chemical analogues in the PBPK knowledgebase can guide the construction of PBPK models for other chemicals. C1 [Lu, Jingtao; Leonard, Jeremy A.; Phillips, Martin B.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Goldsmith, Michael-Rock; Grulke, Christopher M.; Chang, Daniel T.; Brooks, Raina D.; Hypes, Ethan D.; Fair, Matthew J.; Tornero-Velez, Rogelio; Tan, Yu-Mei] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Hypes, Ethan D.] N Carolina State Univ, Dept Environm Engn, Raleigh, NC 27695 USA. [Fair, Matthew J.] Univ N Carolina, Dept Phys & Phys Oceanog, Wilmington, NC 28401 USA. [Johnson, Jeffre; Dary, Curtis C.] US EPA, Natl Exposure Res Lab, Las Vegas, NV 89193 USA. [Goldsmith, Michael-Rock; Chang, Daniel T.] Chem Comp Grp, Montreal, PQ, Canada. [Grulke, Christopher M.] Lockheed Martin, Res Triangle Pk, NC USA. [Brooks, Raina D.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Phillips, Martin B.] Minnesota Dept Hlth, St Paul, MN USA. [Dary, Curtis C.] Insiliconomics, Birchwood, WI USA. RP Grulke, CM; Tan, YM (reprint author), US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA.; Grulke, CM (reprint author), Lockheed Martin, Res Triangle Pk, NC USA. EM grulke.chris@epa.gov; tan.cecilia@epa.gov OI Phillips, Martin/0000-0002-6282-529X FU Oak Ridge Institute for Science and Education's Research Participation Program at the US Environmental Protection Agency FX JL, JAL and MBP were funded by the Oak Ridge Institute for Science and Education's Research Participation Program at the US Environmental Protection Agency. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 97 TC 0 Z9 0 U1 0 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-734X EI 1553-7358 J9 PLOS COMPUT BIOL JI PLoS Comput. Biol. PD FEB PY 2016 VL 12 IS 2 AR e1004495 DI 10.1371/journal.pcbi.1004495 PG 22 WC Biochemical Research Methods; Mathematical & Computational Biology SC Biochemistry & Molecular Biology; Mathematical & Computational Biology GA DM8AL UT WOS:000376582900020 PM 26871706 ER PT J AU Nye, MB Mulvaney, KK AF Nye, Michael B. Mulvaney, Kate K. TI Who is Next? Identifying Communities with the Potential for Increased Implementation of Sustainability Policies and Programs SO SUSTAINABILITY LA English DT Article ID CLIMATE PROTECTION; UNITED-STATES; US CITIES; VULNERABILITY; TYPOLOGY; CAPACITY; INITIATIVES; ADOPTION C1 [Nye, Michael B.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Denver, CO 80202 USA. [Mulvaney, Kate K.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Lab, Atlantic Ecol Div, Narragansett, RI 02882 USA. RP Nye, MB (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab, Denver, CO 80202 USA. EM nye.michael@epa.gov; mulvaney.kate@epa.gov NR 41 TC 0 Z9 0 U1 2 U2 4 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD FEB PY 2016 VL 8 IS 2 AR 182 DI 10.3390/su8020182 PG 18 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA DG1LR UT WOS:000371830100023 ER PT J AU Lannigan, J Nolan, JP Zucker, R AF Lannigan, Joanne Nolan, John P. Zucker, Robert TI Measurement of Extracellular Vesicles and Other Submicron Size Particles by Flow Cytometry SO CYTOMETRY PART A LA English DT Editorial Material ID EXOSOMES C1 [Lannigan, Joanne] Univ Virginia, Sch Med, Charlottesville, VA 22908 USA. [Nolan, John P.] Scintillon Inst, San Diego, CA USA. [Zucker, Robert] US EPA, Toxicol Assessment Div, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. RP Lannigan, J (reprint author), Univ Virginia, Sch Med, Charlottesville, VA 22908 USA.; Nolan, JP (reprint author), Scintillon Inst, San Diego, CA USA.; Zucker, R (reprint author), US EPA, Toxicol Assessment Div, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. EM jl7fj@virginia.edu; jnolan@scintillon.org; Zucker.robert@epa.gov NR 5 TC 3 Z9 3 U1 4 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1552-4922 EI 1552-4930 J9 CYTOM PART A JI Cytom. Part A PD FEB PY 2016 VL 89A IS 2 SI SI BP 109 EP 110 DI 10.1002/cyto.a.22814 PG 2 WC Biochemical Research Methods; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA DK2AB UT WOS:000374715900001 PM 26889762 ER PT J AU Zucker, RM Ortenzio, JNR Boyes, WK AF Zucker, Robert M. Ortenzio, Jayna N. R. Boyes, William K. TI Characterization, Detection, and Counting of Metal Nanoparticles Using Flow Cytometry SO CYTOMETRY PART A LA English DT Article DE flow cytometry; nanoparticles; submicron particles; swarm; silver nanoparticles; gold nanoparticles; gold; silver; TiO2; side scatter; forward scatter; fluorescence ID CALIBRATED POLYSTYRENE BEADS; CIRCULATING MICROPARTICLES; BIOLOGICAL MICROPARTICLES; SILVER NANOPARTICLES; GOLD NANOPARTICLES; LIGHT-SCATTERING; VESICLES; SIZE; STANDARDIZATION; CELLS AB There is a need to accurately detect, characterize, and quantify nanoparticles in suspensions. This study helps to understand the complex interactions between similar types of nanoparticles. Before initiating a study of metal nanoparticles, five submicron PS beads with sizes between 200 nm and 1 mu m were used to derive a reference scale that was useful in evaluating the flow cytometer for functionality, sensitivity, resolution, and reproducibility. Side scatter intensity (SSC) from metal nanoparticles was obtained simultaneously from 405 nm and 488 nm lasers. The 405 nm laser generally yielded histogram distributions with smaller CVs, less side scatter intensity, better separation indices between beads and decreased scatter differences between different sized particles compared with the 488 nm laser. Submicron particles must be diluted to 10(6) and 10(7) particles/mL before flow cytometer analysis to avoid coincidence counting artifacts. When particles were too concentrated the following occurred: swarm, electronic overload, coincidence counting, activation of doublet discrimination and rejection circuitry, increase of mean SSC histogram distributions, alterations of SSC and pulse width histogram shape, decrease and fluctuations in counting rate and decrease or elimination of particulate water noise and 1 mu m reference bead. To insure that the concentrations were in the proper counting range, the nanoparticle samples were mixed with a known concentration of 1 mu m counting beads. Sequential dilutions of metal nanoparticles in a 1 mu m counting bead suspension helped determine the diluted concentration needed for flow cytometer analysis. It was found that the original concentrated nanoparticle samples had to be diluted, between 1: 10,000 and 1: 100,000, before characterization by flow cytometry. The concentration of silver or gold nanoparticles in the undiluted sample were determined by comparing them with a known concentration (1.9 x 10(6) beads/mL) of 1 mu m polystyrene reference beads. Published 2015Wiley Periodicals Inc., on behalf of ISAC C1 [Zucker, Robert M.; Boyes, William K.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Toxicol Assessment Div, MD B105-04, Res Triangle Pk, NC 27711 USA. [Ortenzio, Jayna N. R.] US EPA, Natl Hlth & Environm Effects Res Lab, ORISE Appointee, Res Triangle Pk, NC 27711 USA. RP Zucker, RM (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Toxicol Assessment Div, MD B105-04, Res Triangle Pk, NC 27711 USA. EM zucker.robert@epa.gov FU United States Environmental Protection Agency FX This research was supported in part by an appointment to the Research Participation Program for the U.S. Environmental Protection Agency, Office of Research and Development, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. Research described in this article was supported by the United States Environmental Protection Agency; it has been subjected to Agency review but does not necessarily reflect the views of the Agency. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 57 TC 4 Z9 4 U1 6 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1552-4922 EI 1552-4930 J9 CYTOM PART A JI Cytom. Part A PD FEB PY 2016 VL 89A IS 2 SI SI BP 169 EP 183 DI 10.1002/cyto.a.22793 PG 15 WC Biochemical Research Methods; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA DK2AB UT WOS:000374715900007 PM 26619039 ER PT J AU Han, C Lalley, J Namboodiri, D Cromer, K Nadagouda, MN AF Han, Changseok Lalley, Jacob Namboodiri, Devi Cromer, Keeley Nadagouda, Mallikarjuna N. TI Titanium dioxide-based antibacterial surfaces for water treatment SO CURRENT OPINION IN CHEMICAL ENGINEERING LA English DT Article ID AG-DOPED TIO2; ESCHERICHIA-COLI; MICROCYSTIN-LR; PSEUDOMONAS-AERUGINOSA; E. COLI; DISINFECTION; FILMS; PHOTOCATALYSIS; INACTIVATION; DEGRADATION AB The field of water disinfection has gained much interest since waterborne diseases caused by pathogenic microorganisms directly endanger human health. Antibacterial surfaces offer a new, ecofriendly technique to reduce the harmful disinfection byproducts that form in medical and food processing industries. Titanium dioxide (TiO2) photocatalysts have been extensively studied to prepare antibacterial surfaces due to their environmentally favorable properties. The studies demonstrate TiO2 improves the efficiency of disinfection by the effective inactivation of pathogenic microorganisms (i.e., Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Pseudomonas putida, and Listeria innocua). TiO2 photocatalysts decompose natural algal toxins such as microcystin-LR and cylindrospermopsin under solar light irradiation. On the basis of literature review, these antibacterial surfaces may be applied to hospital, food, ceramic, and building industries or to environmental remediation where bacteria inactivation is required to ensure the safety of human health and the environment. C1 [Han, Changseok] US EPA, ORD, NRMRL, STD,CPB, 26 W Martin Luther King Jr Dr, Cincinnati, OH 45268 USA. [Lalley, Jacob; Cromer, Keeley; Nadagouda, Mallikarjuna N.] US EPA, ORD, NRMRL, WSWRD,WQMB, 26 W Martin Luther King Jr Dr, Cincinnati, OH 45268 USA. [Namboodiri, Devi] Seven Hills High Sch, 5400 Red Bank Rd, Cincinnati, OH 45227 USA. RP Nadagouda, MN (reprint author), US EPA, ORD, NRMRL, WSWRD,WQMB, 26 W Martin Luther King Jr Dr, Cincinnati, OH 45268 USA. EM nadagouda.mallikarjuna@epa.gov FU U.S. Environmental Protection Agency, through its Office of Research and Development FX The U.S. Environmental Protection Agency, through its Office of Research and Development, funded and managed, or partially funded and collaborated in, the research described herein. It has been subjected to the Agency's administrative review and has been approved for external publication. Any opinions expressed in this paper are those of the author(s) and do not necessarily reflect the views of the Agency, therefore, no official endorsement should be inferred. Any mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 47 TC 1 Z9 1 U1 14 U2 19 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 2211-3398 J9 CURR OPIN CHEM ENG JI Curr. Opin. Chem. Eng. PD FEB PY 2016 VL 11 BP 46 EP 51 DI 10.1016/j.coche.2015.11.007 PG 6 WC Biotechnology & Applied Microbiology; Engineering, Chemical SC Biotechnology & Applied Microbiology; Engineering GA DL1QM UT WOS:000375406800010 ER PT J AU Croston, TL Nayak, AP Lemons, AR Goldsmith, WT Kashon, ML Germolec, DM Beezhold, DH Green, BJ AF Croston, Tara L. Nayak, Ajay P. Lemons, Angela R. Goldsmith, W. Travis Kashon, Michael L. Germolec, Dori M. Beezhold, Donald H. Green, Brett J. TI Pulmonary MicroRNA Expression Profiles Associated with Subchronic Aspergillus fumigatus Exposure SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY MAR 04-07, 2016 CL Los Angeles, CA SP Amer Acad Allergy, Asthma & Immunol C1 [Croston, Tara L.; Nayak, Ajay P.; Lemons, Angela R.; Green, Brett J.] NIOSH, Allergy & Clin Immunol Branch, Hlth Effects Lab Div, Ctr Dis Control & Prevent, Morgantown, WV USA. [Goldsmith, W. Travis] NIOSH, Engn & Control Technol Branch, Hlth Effects Lab Div, Ctr Dis Control & Prevent, Morgantown, WV USA. [Kashon, Michael L.] NIOSH, Biostat & Epidemiol Branch, Hlth Effects Lab Div, Ctr Dis Control & Prevent, Morgantown, WV USA. [Germolec, Dori M.] Natl Inst Environm Hlth Sci, Toxicol Branch, Div Natl Toxicol Program, Res Triangle Pk, NC USA. [Beezhold, Donald H.] NIOSH, Hlth Effects Lab Div, Ctr Dis Control & Prevent, Morgantown, WV USA. NR 0 TC 0 Z9 0 U1 3 U2 3 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2016 VL 137 IS 2 SU S MA 889 BP AB272 EP AB272 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA DK6BI UT WOS:000375005404085 ER PT J AU Hall, TMT AF Hall, Traci M. Tanaka TI De-coding and re-coding RNA recognition by PUF and PPR repeat proteins SO CURRENT OPINION IN STRUCTURAL BIOLOGY LA English DT Article ID PUMILIO-HOMOLOGY DOMAIN; SINGLE-STRANDED RNA; BINDING-SPECIFICITY; STRUCTURAL BASIS; MODULAR RECOGNITION; MITOCHONDRIAL RNA; SEQUENCE; SCAFFOLD; CODE; ARABIDOPSIS AB PUF and PPR proteins are two families of alpha-helical repeat proteins that recognize single-stranded RNA sequences. Both protein families hold promise as scaffolds for designed RNA binding domains. A modular protein RNA recognition code was apparent from the first crystal structures of a PUF protein in complex with RNA, and recent studies continue to advance our understanding of natural PUF protein recognition (de-coding) and our ability to engineer specificity (re-coding). Degenerate recognition motifs make de-coding specificity of individual PPR proteins challenging. Nevertheless, re-coding PPR protein specificity using a consensus recognition code has been successful. C1 [Hall, Traci M. Tanaka] Natl Inst Environm Hlth Sci, Epigenet & Stem Cell Biol Lab, NIH, Res Triangle Pk, NC 27709 USA. RP Hall, TMT (reprint author), Natl Inst Environm Hlth Sci, Epigenet & Stem Cell Biol Lab, NIH, Res Triangle Pk, NC 27709 USA. EM hall4@niehs.nih.gov FU Intramural Research Program of the National Institutes of Health, National Institute of Environmental Health Sciences FX I am grateful for the input of my colleagues at NIEHS, G Mueller and T Teramoto. This work was supported by the Intramural Research Program of the National Institutes of Health, National Institute of Environmental Health Sciences. NR 49 TC 2 Z9 2 U1 1 U2 5 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0959-440X EI 1879-033X J9 CURR OPIN STRUC BIOL JI Curr. Opin. Struct. Biol. PD FEB PY 2016 VL 36 BP 116 EP 121 DI 10.1016/j.sbi.2016.01.010 PG 6 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA DH3IR UT WOS:000372681200016 PM 26874972 ER PT J AU Lake, AD Wood, CE Bhat, VS Chorley, BN Carswell, GK Sey, YM Kenyon, EM Padnos, B Moore, TM Tennant, AH Schmid, JE George, BJ Ross, DG Hughes, MF Corton, JC Simmons, JE McQueen, CA Hester, SD AF Lake, April D. Wood, Charles E. Bhat, Virunya S. Chorley, Brian N. Carswell, Gleta K. Sey, Yusupha M. Kenyon, Elaina M. Padnos, Beth Moore, Tanya M. Tennant, Alan H. Schmid, Judith E. George, Barbara Jane Ross, David G. Hughes, Michael F. Corton, J. Christopher Simmons, Jane Ellen McQueen, Charlene A. Hester, Susan D. TI Dose and Effect Thresholds for Early Key Events in a PPAR alpha-Mediated Mode of Action SO TOXICOLOGICAL SCIENCES LA English DT Article DE mode of action; adverse outcome pathway; benchmark dose; peroxisome proliferator-activated receptor-alpha; liver carcinogenesis; phthalate ID CHEMICAL RISK-ASSESSMENT; ANDROSTANE RECEPTOR CAR; HUMAN RELEVANCE; FRAMEWORK; MICE; CONCORDANCE; PHTHALATE; TOXICITY; PATHWAY; DI(2-ETHYLHEXYL)PHTHALATE AB Current strategies for predicting adverse health outcomes of environmental chemicals are centered on early key events in toxicity pathways. However, quantitative relationships between early molecular changes in a given pathway and later health effects are often poorly defined. The goal of this study was to evaluate short-term key event indicators using qualitative and quantitative methods in an established pathway of mouse liver tumorigenesis mediated by peroxisome proliferator-activated receptor alpha (PPAR alpha). Male B6C3F1 mice were exposed for 7 days to di (2-ethylhexyl) phthalate (DEHP), di-n-octyl phthalate (DNOP), and n-butyl benzyl phthalate (BBP), which vary in PPAR alpha activity and liver tumorigenicity. Each phthalate increased expression of select PPARa target genes at 7 days, while only DEHP significantly increased liver cell proliferation labeling index (LI). Transcriptional benchmark dose (BMDT) estimates for dose-related genomic markers stratified phthalates according to hypothetical tumorigenic potencies, unlike BMDs for non-genomic endpoints (relative liver weights or proliferation). The 7-day BMDT values for Acot1 as a surrogate measure for PPAR alpha activation were 29, 370, and 676 mg/kg/day for DEHP, DNOP, and BBP, respectively, distinguishing DEHP (liver tumor BMD of 35 mg/kg/day) from non-tumorigenic DNOP and BBP. Effect thresholds were generated using linear regression of DEHP effects at 7 days and 2-year tumor incidence values to anchor early response molecular indicators and a later phenotypic outcome. Thresholds varied widely by marker, from 2-fold (Pdk4 and proliferation LI) to 30-fold (Acot1) induction to reach hypothetical tumorigenic expression levels. These findings highlight key issues in defining thresholds for biological adversity based on molecular changes. C1 [Lake, April D.] Univ N Carolina, Curriculum Toxicol, Chapel Hill, NC 27599 USA. [Lake, April D.] US EPA, ORD, NHEERL, ORISE, Res Triangle Pk, NC 27711 USA. [Lake, April D.; Wood, Charles E.; Chorley, Brian N.; Carswell, Gleta K.; Sey, Yusupha M.; Kenyon, Elaina M.; Padnos, Beth; Moore, Tanya M.; Tennant, Alan H.; Ross, David G.; Hughes, Michael F.; Corton, J. Christopher; Simmons, Jane Ellen; McQueen, Charlene A.; Hester, Susan D.] US EPA, ORD, NHEERL, Integrated Syst Toxicol Div, Res Triangle Pk, NC 27711 USA. [Bhat, Virunya S.] NSF Int, Ann Arbor, MI 48105 USA. [Schmid, Judith E.] US EPA, ORD, NHEERL, Toxicol Assessment Div, Res Triangle Pk, NC 27711 USA. [George, Barbara Jane] US EPA, ORD, NHEERL, Off Associate Director Hlth, Res Triangle Pk, NC 27711 USA. RP Hester, SD (reprint author), US EPA, ORD, NHEERL, Integrated Syst Toxicol Div, Res Triangle Pk, NC 27711 USA. EM hester.susan@epa.gov FU U.S. Environmental Protection Agency Office of Research and Development FX U.S. Environmental Protection Agency Office of Research and Development. NR 42 TC 2 Z9 2 U1 3 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD FEB PY 2016 VL 149 IS 2 BP 312 EP 325 DI 10.1093/toxsci/kfv236 PG 14 WC Toxicology SC Toxicology GA DF8NF UT WOS:000371613900008 PM 26519955 ER PT J AU Lynam, MM Dvonch, JT Barres, JA Landis, MS Kamal, AS AF Lynam, Mary M. Dvonch, J. Timothy Barres, James A. Landis, Matthew S. Kamal, Ali S. TI Investigating the impact of local urban sources on total atmospheric mercury wet deposition in Cleveland, Ohio, USA SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Mercury deposition network; Meteorological case study; Lake Erie ID DRY DEPOSITION; LAKE MICHIGAN; EASTERN OHIO; PRECIPITATION; VARIABILITY; TRANSPORT; ILLINOIS AB Event-based precipitation samples were collected at a downtown industrial and a predominantly upwind rural location in the Cleveland, Ohio metropolitan area from July 2009 through December 2010 to investigate the potential local total mercury (Hg) wet deposition enhancement in a region with a high concentration of coal combustion sources. Total Hg wet deposition for the 18 -month period was 6.8 mu g m(-2) (n = 81) at the rural site and 10.7 mu g m(-2) (n = 98) at the urban site demonstrating a significant (p = 0.046) 37% enhancement in deposition between the two sites. Large deposition events (>0.2 mu g m(-2)) occurred predominantly from May through October (n = 16 (urban), n = 10 (rural)) and represented from 2 to 8% of total 18-month deposition per event. At the downtown urban site, the average Hg precipitation concentration was 53% higher for these large deposition events. Concurrently measured precipitation events delivered in aggregate 2.4 times more total Hg wet deposition to the urban site compared to the rural site. Hg rainfall concentrations for concurrent events with similar precipitation depth were 2-4 times higher at the urban site and suggest scavenging of local Hg emissions. Further evaluation of these events revealed 83% more total Hg deposition at the urban site from January to December 2010 compared to July to December 2009, while there was 26% more at the rural site during these same time periods. The larger increase in deposition at the urban site in 2010 may be evidence of increased local emissions from sources that were known to be offline during this study period because of an economic recession. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Lynam, Mary M.; Dvonch, J. Timothy; Barres, James A.] Univ Michigan, Air Qual Lab, Ann Arbor, MI 48109 USA. [Landis, Matthew S.; Kamal, Ali S.] US EPA, Off Res & Dev, Res Triangle Pk, NC 27711 USA. [Kamal, Ali S.] US EPA, Off Air Qual Planning & Stand, Res Triangle Pk, NC 27711 USA. RP Lynam, MM (reprint author), Univ Michigan, Air Qual Lab, Ann Arbor, MI 48109 USA. EM lynam@umich.edu RI Dvonch, Joseph/K-3632-2013 FU U.S. Environmental Protection Agency (EPA) through its Office of Research and Development; Alion Science and Technology [EP-D-05-065]; University of Michigan Air Quality Laboratory [003230-002-BDM] FX The U.S. Environmental Protection Agency (EPA) through its Office of Research and Development funded, managed, and participated in this research described here under (i) contract EP-D-05-065 with Alion Science and Technology, and (ii) subcontract 003230-002-BDM with the University of Michigan Air Quality Laboratory. The views expressed in this paper are those of the authors and do not necessarily reflect the views or policies of EPA. It has been subjected to Agency review and approved for publication. Mention of trade names or commercial products do not constitute an endorsement or recommendation for use.The authors thank Mike Wheeler and Keith Kronmiller (Alion) for their logistical assistance in site construction; Sam Rubens and Jim Eckert (Ohio Environmental Protection Agency) for LCM site operations; and Marv Rogers, Myles Murray, Carlton See, and Miro Gnjatil (City of Cleveland Department of Public Health - Division of Air Quality) for GTC site operations. We are very grateful to the anonymous reviewers whose comprehensive comments and suggestions contributed to improving this manuscript. NR 30 TC 2 Z9 2 U1 3 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD FEB PY 2016 VL 127 BP 262 EP 271 DI 10.1016/j.atmosenv.2015.12.048 PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DE6VC UT WOS:000370770700030 ER PT J AU Glaser, J AF Glaser, John A. TI Nerve gas destruction with metal organic frameworks SO CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY LA English DT News Item ID ANTIDIABETIC DRUG METFORMIN; CHEMICAL-WARFARE AGENTS; DRINKING-WATER; DEGRADATION; PROPRANOLOL; ENVIRONMENT; HYDROLYSIS; GUANYLUREA; SIMULANTS; FATE C1 [Glaser, John A.] US EPA, Natl Risk Management Res Lab, 26 W King Dr, Cincinnati, OH 45268 USA. RP Glaser, J (reprint author), US EPA, Natl Risk Management Res Lab, 26 W King Dr, Cincinnati, OH 45268 USA. EM Glaser.John@epa.gov NR 19 TC 0 Z9 0 U1 5 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1618-954X EI 1618-9558 J9 CLEAN TECHNOL ENVIR JI Clean Technol. Environ. Policy PD FEB PY 2016 VL 18 IS 2 BP 351 EP 358 DI 10.1007/s10098-016-1108-0 PG 8 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA DE7LY UT WOS:000370819500002 ER PT J AU Tu, QS Lu, MM Yang, YJ Scott, D AF Tu, Qingshi Lu, Mingming Yang, Y. Jeffrey Scott, Don TI Water consumption estimates of the biodiesel process in the US SO CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY LA English DT Article DE Biodiesel; Water consumption; Irrigation; Soybean crushing and processing; Water-stressed areas ID LIFE-CYCLE ASSESSMENT; COOKING OIL AB As a renewable alternative to petroleum diesel, biodiesel has been widely used in the US and the world. However, its potential impact on water resources has not been much evaluated. This study investigates water consumption from the biodiesel process, which includes three stages: soybean irrigation, soybean-to-soybean oil processing, and biodiesel manufacturing, at both national and state levels. Mass-based allocation is performed and water consumption at the three stages is obtained on the basis of million gallons per year and gallon water per gallon biodiesel (gal/gal). The normalized water consumption (water intensity) of the irrigation, oil processing, and biodiesel production stages are 61.78, 0.17, and 0.31 gal/gal, respectively. The resulting total normalized water consumption is 62.26 gal/gal for the biodiesel process which is much lower than those reported in existing literature. It is shown that water consumption from the three stages varies significantly from state to state, which warrants the necessity of state-level water consumption analysis for better decision making in water resources management. Water consumption in potentially water-stressed states is also investigated and results show that currently these states represent 1.6 % of total water consumption associated with biodiesel production, 0.46 % of soybean harvest, and 27.61 % of biodiesel production capacity in the US. C1 [Tu, Qingshi; Lu, Mingming] Univ Cincinnati, Dept Biol Chem & Environm Engn, POB 210071, Cincinnati, OH 45221 USA. [Yang, Y. Jeffrey] US EPA, Natl Risk Management Res Labs, Cincinnati, OH 45268 USA. [Scott, Don] Natinoal Biodiesel Board, Columbia, MO USA. RP Lu, MM (reprint author), Univ Cincinnati, Dept Biol Chem & Environm Engn, POB 210071, Cincinnati, OH 45221 USA. EM lumg@ucmail.uc.edu FU National Risk Management Research Laboratory, U.S. Environmental Protection Agency (US EPA) [PEG4-52] FX Funding to support the study from the National Risk Management Research Laboratory, U.S. Environmental Protection Agency (US EPA contract PEG4-52) is acknowledged. Help from the following individuals is also acknowledged: Dr. Gary Knothe and Dr. Michael Haas from the UDSA, Tom O'Conner from NBB, Dr. Ronald Pate (Sandia National Laboratory), Jane Bare (USEPA), William Smith, Klaus Ruhmer and Dr. Ming Chai (the biodiesel industry), and Junsong Zhang (UC). NR 30 TC 2 Z9 2 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1618-954X EI 1618-9558 J9 CLEAN TECHNOL ENVIR JI Clean Technol. Environ. Policy PD FEB PY 2016 VL 18 IS 2 BP 507 EP 516 DI 10.1007/s10098-015-1032-8 PG 10 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA DE7LY UT WOS:000370819500013 ER PT J AU Aitken, ML Loughlin, DH Dodder, RS Yelverton, WH AF Aitken, Matthew L. Loughlin, Daniel H. Dodder, Rebecca S. Yelverton, William H. TI Economic and environmental evaluation of coal-and-biomass-to-liquids-and-electricity plants equipped with carbon capture and storage SO CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY LA English DT Article DE MARKet ALlocation (MARKAL) energy system model; Fischer-Tropsch liquid fuels; Gasification; Electricity generation; Coal; Biomass; Carbon capture and sequestration ID GASIFICATION; PERFORMANCE; FUELS; FACILITIES; CLIMATE; TRANSPORTATION; INTEGRATION; SCENARIOS; EMISSIONS; OPTIONS AB Among various clean energy technologies, one innovative option for reducing the emission of greenhouse gases (GHGs) and criteria pollutants involves pairing carbon capture and storage (CCS) with the production of synthetic fuels and electricity from a combination of coal and sustainably sourced biomass. With a relatively pure CO2 stream as an inherent byproduct of the process, most of the resulting GHG emissions can be eliminated by simply compressing the CO2 for pipeline transport. Subsequent storage of the CO2 output in underground reservoirs can result in very low-perhaps even near-zero-net GHG emissions, depending on the fraction of biomass as input and its CO2 signature. To examine the potential market penetration and environmental impact of coal-and-biomass-to-liquids-and-electricity (CBtLE), a system-wide sensitivity analysis was performed using the MARKet ALlocation energy model. CBtLE was found to be most competitive in scenarios with a combination of high oil prices, low CCS costs, and, unexpectedly, non-stringent carbon policies. In the scheme considered here (30 % biomass input on an energy basis and 85 % carbon capture), CBtLE fails to achieve significant market share in deep decarbonization scenarios, regardless of oil prices and CCS costs. Such facilities would likely require higher fractions of biomass feedstock and captured CO2 to successfully compete in a carbon-constrained energy system. C1 [Aitken, Matthew L.] US EPA, ORISE, Res Triangle Pk, NC 27709 USA. [Loughlin, Daniel H.; Dodder, Rebecca S.; Yelverton, William H.] US EPA, Res Triangle Pk, NC 27709 USA. RP Loughlin, DH (reprint author), US EPA, Res Triangle Pk, NC 27709 USA. EM loughlin.dan@epa.gov NR 46 TC 2 Z9 2 U1 1 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1618-954X EI 1618-9558 J9 CLEAN TECHNOL ENVIR JI Clean Technol. Environ. Policy PD FEB PY 2016 VL 18 IS 2 BP 573 EP 581 DI 10.1007/s10098-015-1020-z PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA DE7LY UT WOS:000370819500019 ER PT J AU Heinrichs, JA Lawler, JJ Schumaker, NH AF Heinrichs, Julie A. Lawler, Joshua J. Schumaker, Nathan H. TI Intrinsic and extrinsic drivers of source-sink dynamics SO ECOLOGY AND EVOLUTION LA English DT Article DE Habitat quality; individual-based model; landscape pattern; population growth; source-sink dynamics; stochasticity ID HETEROGENEOUS LANDSCAPES; POPULATION PERSISTENCE; SPATIAL HETEROGENEITY; HABITAT QUALITY; SCALE; METAPOPULATIONS; CONNECTIVITY; DEMOGRAPHY; DISPERSAL; EVOLUTION AB Many factors affect the presence and exchange of individuals among subpopulations and influence not only the emergence, but the strength of ensuing source-sink dynamics within metapopulations. Yet their relative contributions remain largely unexplored. To help identify the characteristics of empirical systems that are likely to exhibit strong versus weak source-sink dynamics and inform their differential management, we compared the relative roles of influential factors in strengthening source-sink dynamics. In a series of controlled experiments within a spatially explicit individual-based model framework, we varied patch quality, patch size, the dispersion of high- and low-quality patches, population growth rates, dispersal distances, and environmental stochasticity in a factorial design. We then recorded source-sink dynamics that emerged from the simulated habitat and population factors. Long-term differences in births and deaths were quantified for sources and sinks in each system and used in a statistical model to rank the influences of key factors. Our results suggest that systems with species capable of rapid growth, occupying habitat patches with more disparate qualities, with interspersed higher- and lower-quality habitats, and that experience relatively stable environments (i.e., fewer negative perturbations) are more likely to exhibit strong source-sink dynamics. Strong source-sink dynamics emerged under diverse combinations of factors, suggesting that simple inferences of process from pattern will likely be inadequate to predict and assess the strength of source-sink dynamics. Our results also suggest that it may be more difficult to detect and accurately measure source-sink dynamics in slow-growing populations, highly variable environments, and where a subtle gradient of habitat quality exists. C1 [Heinrichs, Julie A.; Lawler, Joshua J.] Univ Washington, Sch Environm & Forest Sci, POB 352100, Seattle, WA 98195 USA. [Schumaker, Nathan H.] US EPA, Western Ecol Div, 200 SW 35th St, Corvallis, OR 97333 USA. RP Heinrichs, JA (reprint author), Univ Washington, Sch Environm & Forest Sci, POB 352100, Seattle, WA 98195 USA. EM jheinrx@u.washington.edu FU Strategic Environmental Research and Development Program (SERDP) [RC-2120]; U.S. Environmental Protection Agency FX This work was funded by the Strategic Environmental Research and Development Program (SERDP) as part of project RC-2120. The information in this document has been funded in part by the U.S. Environmental Protection Agency. NR 39 TC 3 Z9 3 U1 12 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD FEB PY 2016 VL 6 IS 4 BP 892 EP 904 DI 10.1002/ece3.2029 PG 13 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DF1AC UT WOS:000371069800003 PM 26941935 ER PT J AU Fonseca, AMP Marques, CAF Pinto-Correia, T Campbell, DE AF Fonseca, Ana Margarida P. Marques, Carlos A. F. Pinto-Correia, Teresa Campbell, Daniel E. TI Emergy analysis of a silvo-pastoral system, a case study in southern Portugal SO AGROFORESTRY SYSTEMS LA English DT Article DE Montado; Emergy; Cattle rearing; Multifunctionality; Intensification ID OAK; SUSTAINABILITY; REGENERATION; ECOSYSTEM; MONTADO; AGROFORESTRY; CONTINENTS; VEGETATION; OCEANS AB The Mediterranean silvo-pastoral system known as Montado, in Portugal, is a complex land use system composed of an open tree stratum in various densities and an herbaceous layer, used for livestock grazing. Livestock also profit from the acorns, and the grazing contributes to avoid shrub encroachment. In the last 20 years, subsidies from the European Union have greatly promoted cattle rearing in this system and the introduction of heavy breeds, at the expense of sheep, goats or the native cattle breeds. The balance of the traditional system is thus threatened, and a precise assessment of the balance between the different components of the system, therefore is highly needed. The goal of this study was to gain a better understanding of a Montado farm system with cattle rearing as the major economic activity by applying the emergy evaluation method to calculate indices of yield, investment, environmental loading and sustainability. By integrating different ecosystem components, the emergy evaluation method allows a comprehensive evaluation of this complex and multifunctional system at the scale of an individual farm. This method provides a set of indices that can help us understand the system and design management strategies that maximize emergy flow in the farm. In this paper, we apply the emergy evaluation method to a Montado farm with cattle rearing, as a way to gain a better understanding of this system at the farm scale. The value for the transformity of veal (2.66E+06 sej J(-1)) is slightly higher, when compared to other systems producing protein. That means that the investment of nature and man in this product was higher and it requires a premium price on the market. The renewability for Holm Oaks Farm (49 %), lower than for other similar systems, supports the assumption that this is a farm in which, comparing with others, the number of purchased inputs in relation to renewable inputs provided by nature, is higher. The Emergy Investment Ratio is 0.91 for cattle rearing compared to a value of 0.49 for cork and 0.43 for firewood harvesting, making it clear that cattle rearing is a more labor demanding activity comparing with extractive activities as cork and firewood harvesting. C1 [Fonseca, Ana Margarida P.; Pinto-Correia, Teresa] Univ Evora, ICAAM, Polo Mitra,Ap 94, P-7006554 Evora, Portugal. [Fonseca, Ana Margarida P.; Marques, Carlos A. F.] Univ Evora, CEFAGE, Ctr Adv Studies Management & Econ, P-7000 Evora, Portugal. [Campbell, Daniel E.] US EPA, 27 Tarzwell Dr, Narragansett, RI 02882 USA. RP Fonseca, AMP (reprint author), Univ Evora, ICAAM, Polo Mitra,Ap 94, P-7006554 Evora, Portugal. EM anafonseca@uevora.pt FU Fundacao para a Ciencia e Tecnologia (Foundation for Science and Technology) [SFRH/BD/76814/2011]; FEDER through Operational Programme for Competitiveness Factors-COMPETE; National through FCT-Foundation for Science and Technology [PEst-C/AGR/UI0115/2011, PEst-C/QUI/UI0062/2011] FX We are grateful to Centro de Geofisica de Evora (Evora Geophysics Center) for their kind help in the collection of meteorological data and to the Soil Laboratory of University of Evora for the soil analyses. We are also grateful to Carlos Oliveira, from Lusiada University, for sharing his determination of the Emergy to Money Ratio for Portugal for the year 2012. This work has been supported by Fundacao para a Ciencia e Tecnologia (Foundation for Science and Technology) through a Ph.D. grant attributed to Ana Fonseca (SFRH/BD/76814/2011). This work was funded by FEDER Funds through the Operational Programme for Competitiveness Factors-COMPETE and National Funds through FCT-Foundation for Science and Technology under the Strategic Projects PEst-C/AGR/UI0115/2011 and PEst-C/QUI/UI0062/2011. NR 85 TC 0 Z9 0 U1 7 U2 25 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4366 EI 1572-9680 J9 AGROFOREST SYST JI Agrofor. Syst. PD FEB PY 2016 VL 90 IS 1 SI SI BP 137 EP 157 DI 10.1007/s10457-015-9888-5 PG 21 WC Agronomy; Forestry SC Agriculture; Forestry GA DE5YV UT WOS:000370710300012 ER PT J AU Fulford, R Yoskowitz, D Russell, M Dantin, D Rogers, J AF Fulford, Richard Yoskowitz, David Russell, Marc Dantin, Darrin Rogers, John TI Habitat and recreational fishing opportunity in Tampa Bay: Linking ecological and ecosystem services to human beneficiaries SO ECOSYSTEM SERVICES LA English DT Article DE Tampa Bay; Estuarine habitat; Fishing; Valuation; Ecosystem management ID ECONOMIC VALUATION; SEAGRASS; FISHERIES; MODEL; VULNERABILITY; ENVIRONMENT; ESTUARIES; SURVIVAL; MARSHES; GROWTH AB Estimating value of estuarine habitat to human beneficiaries requires that we understand how habitat alteration impacts function through both production and delivery of ecosystem goods and services (EGS). Here we expand on the habitat valuation technique of Bell (1997) with an estimate of recreational angler willingness-to-pay combined with estimates of angler effort, fish population size, and fish and angler distribution. Results suggest species-specific fishery value is impacted by angler interest and stock status, as the most targeted fish (spotted seatrout) did not have the highest specific value (fish(-1)). Reduced population size and higher size at capture resulted in higher specific value for common snook. Habitat value estimated from recreational fishing value and fish-angler distributions supported an association between seagrass and habitat value, yet this relationship was also impacted by distance to access points. This analysis does not provide complete valuation of habitat as it considers only one service (fishing), but demonstrates a methodology to consider functional equivalency of all habitat features as a part of a habitat mosaic rather than in isolation, as well as how to consider both EGS production and delivery to humans (e.g., anglers) in any habitat valuation, which are critical for a transition to ecosystem management. Published by Elsevier B.V. C1 [Fulford, Richard; Russell, Marc; Dantin, Darrin; Rogers, John] US EPA, Gulf Ecol Div, 1 Sabine Isl Rd, Gulf Breeze, FL 32561 USA. [Yoskowitz, David] Texas A&M Univ Corpus Christi, Harte Res Inst, Corpus Christi, TX USA. RP Fulford, R (reprint author), US EPA, Gulf Ecol Div, 1 Sabine Isl Rd, Gulf Breeze, FL 32561 USA. EM Fulford.Richard@epa.gov; David.Yoskowitz@tamucc.edu; Russell.Marc@epa.gov; Dantin.Darrin@epa.gov; Rogers.Johne@epa.gov FU Florida Fish and Wildlife Research Institute; Tampa Bay National Estuary Program FX This work would not have been possible without the cooperation and support of the Florida Fish and Wildlife Research Institute and the Tampa Bay National Estuary Program. We particularly thank David Chagaris and Peter Rubec for providing access to data on fish distribution and stock status and for their assistance with accessing the Marine Recreational Information Program (MRIP) database. The views expressed in this paper are those of the authors and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency. The use of named analytical equipment in this manuscript does not imply endorsement from such corporations. NR 51 TC 1 Z9 1 U1 8 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2212-0416 J9 ECOSYST SERV JI Ecosyst. Serv. PD FEB PY 2016 VL 17 BP 64 EP 74 DI 10.1016/j.ecoser.2015.11.009 PG 11 WC Ecology; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA DE5AC UT WOS:000370641300010 ER PT J AU Liu, ZW Kind, J AF Liu, Ziwei Kind, Joanna TI Pressure Points: Why It Makes Sense to Label Sprinklers With Pressure Regulation SO JOURNAL AMERICAN WATER WORKS ASSOCIATION LA English DT Article C1 [Liu, Ziwei] US EPA, WaterSense Program, ORISE, 1200 Penn Ave NW,7324U, Washington, DC 20460 USA. [Kind, Joanna] Eastern Res Grp Inc, Lexington, MA USA. RP Liu, ZW (reprint author), US EPA, WaterSense Program, ORISE, 1200 Penn Ave NW,7324U, Washington, DC 20460 USA. EM liu.ziwei@epa.gov NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER WATER WORKS ASSOC PI DENVER PA 6666 W QUINCY AVE, DENVER, CO 80235 USA SN 2164-4535 J9 J AM WATER WORKS ASS JI J. Am. Water Work Assoc. PD FEB PY 2016 VL 108 IS 2 BP 36 EP 39 DI 10.5942/jawwa.2016.108.0036 PG 4 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA DD8VQ UT WOS:000370205400008 ER PT J AU Lane, CR Autrey, BC AF Lane, C. R. Autrey, B. C. TI Phosphorus retention of forested and emergent marsh depressional wetlands in differing land uses in Florida, USA SO WETLANDS ECOLOGY AND MANAGEMENT LA English DT Article DE Equilibrium phosphorus concentration; Phosphorus buffering capacity; Phosphorus sorption maximum; S-max; Connectivity; Agriculture; Palustrine wetland ID SURROUNDING PASTURE UPLANDS; FRESH-WATER WETLANDS; UNITED-STATES; AGRICULTURAL PHOSPHORUS; SOILS; SORPTION; QUALITY; SEDIMENTS; CAPACITY; STORAGE AB The translocation of phosphorus (P) from terrestrial landscapes to aquatic bodies is of concern due to the impact of elevated P on aquatic system functioning and integrity. Due to their common location in depressions within landscapes, wetlands, including so-called geographically isolated wetlands (GIWs), receive and process entrained P. The ability of depressional wetlands, or GIWs, to sequester P may vary by wetland type or by land use modality. In this study we quantified three measures of P sorption capacities for two common GIW types (i.e., emergent marsh and forested wetlands) in two different land use modalities (i.e., agricultural and least impacted land uses) across 55 sites in Florida, USA. The equilibrium P concentration (EPC0) averaged 6.42 +/- A 5.18 mg P L-1 (standard deviation reported throughout); and ranged from 0.01-27.18 mg P L-1; there were no differences between GIW type or land use modality, nor interaction effects. Significant differences in phosphorus buffering capacity (PBC) were found between GIW types and land use, but no interaction effects. Forested GIWs [average 306.64 +/- A 229.63 (mg P kg(-1)) (A mu g P L-1)(-1)], and GIWs in agricultural settings [average 269.95 +/- A 236.87 (mg P kg(-1)) (A mu g P L-1)(-1)] had the highest PBC values. The maximum sorption capacity (S-max) was found to only differ by type, with forested wetlands (1274.5 +/- A 1315.7 mg P kg(-1)) having over three times the capacity of emergent GIWs (417.5 +/- A 534.6 mg P kg(-1)). Classification trees suggested GIW soil parameters of bulk density, organic content, and concentrations of total P, H2O-extractable P, and HCl-extractable P were important to classifying GIW P-sorption metrics. We conclude that GIWs have high potential to retain P, but that the entrained P may be remobilized to the wetland water column depending on storm and groundwater input P concentrations. The relative hydrologic dis-connectivity of GIWs from other aquatic systems may provide sufficient retention time to retain elevated P within these systems, thereby providing an ecosystem service to downstream waters. C1 [Lane, C. R.; Autrey, B. C.] US EPA, Off Res & Dev, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. RP Lane, CR (reprint author), US EPA, Off Res & Dev, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM lane.charles@epa.gov FU US Environmental Protection Agency, Office of Research and Development FX All funds to conduct this research originated within the US Environmental Protection Agency, Office of Research and Development. NR 67 TC 0 Z9 0 U1 8 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0923-4861 EI 1572-9834 J9 WETL ECOL MANAG JI Wetl. Ecol. Manag. PD FEB PY 2016 VL 24 IS 1 BP 45 EP 60 DI 10.1007/s11273-015-9450-2 PG 16 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DD8IO UT WOS:000370170100005 ER PT J AU Gilbert, ME Sanchez-Huerta, K Wood, C AF Gilbert, M. E. Sanchez-Huerta, K. Wood, C. TI Mild Thyroid Hormone Insufficiency During Development Compromises Activity-Dependent Neuroplasticity in the Hippocampus of Adult Male Rats SO ENDOCRINOLOGY LA English DT Article ID LONG-TERM POTENTIATION; NEUROTROPHIC FACTOR BDNF; GESTATIONAL MATERNAL HYPOTHYROXINEMIA; INCREASED AUTISM RISK; NERVE GROWTH-FACTOR; GYRUS IN-VIVO; DENTATE GYRUS; MESSENGER-RNA; PROPYLTHIOURACIL PTU; SYNAPTIC PLASTICITY AB Severe thyroid hormone (TH) deficiency during critical phases of brain development results in irreversible neurological and cognitive impairments. The mechanisms accounting for this are likely multifactorial, and are not fully understood. Here we pursue the possibility that one important element is that TH affects basal and activity-dependent neurotrophin expression in brain regions important for neural processing. Graded exposure to propylthiouracil (PTU) during development produced dose-dependent reductions in mRNA expression of nerve growth factor (Ngf) in whole hippocampus of neonates. These changes in basal expression persisted to adulthood despite the return to euthyroid conditions in blood. In contrast to small PTU-induced reductions in basal expression of several genes, developmental PTU treatment dramatically reduced the activity-dependent expression of neurotrophins and related genes (Bdnf(t), Bdnf(iv), Arc, and Klf9) in adulthood and was accompanied by deficits in hippocampal-based learning. These data demonstrate that mild TH insufficiency during development not only reduces expression of important neurotrophins that persists into adulthood but also severely restricts the activity-dependent induction of these genes. Considering the importance of these neurotrophins for sculpting the structural and functional synaptic architecture in the developing and the mature brain, it is likely that TH-mediated deficits in these plasticity mechanisms contribute to the cognitive deficiencies that accompany developmental TH compromise. C1 [Gilbert, M. E.; Wood, C.] US EPA, Tox Assessment Div, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27709 USA. [Sanchez-Huerta, K.] Inst Politecn Nacl, Dept Fisiol Mauricio Russek, Escuela Nacl Ciencias Biol, Mexico City 07738, DF, Mexico. RP Gilbert, ME (reprint author), US EPA, Tox Assessment Div MD B105 05, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA. EM gilbert.mary@epa.gov NR 59 TC 3 Z9 3 U1 3 U2 8 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD FEB PY 2016 VL 157 IS 2 BP 774 EP 787 DI 10.1210/en.2015-1643 PG 14 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DD5LT UT WOS:000369965900031 PM 26606422 ER PT J AU Shi, J Miralles, F Birnbaumer, L Large, WA Albert, AP AF Shi, Jian Miralles, Francesc Birnbaumer, Lutz Large, William A. Albert, Anthony P. TI Store depletion induces Gaq-mediated PLCb1 activity to stimulate TRPC1 channels in vascular smooth muscle cells SO FASEB JOURNAL LA English DT Article DE electrophysiology; PLC activity; Ca2+ signaling; phosphoinositol signaling ID PROTEIN-KINASE-C; OPERATED CA2+ CHANNELS; PORTAL-VEIN MYOCYTES; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; CALCIUM-CHANNELS; ARTERY MYOCYTES; ANGIOTENSIN-II; ACTIVATION; ENTRY; STIM1 AB Depletion of sarcoplasmic reticulum (SR) Ca2+ stores activates store-operated channels (SOCs) composed of canonical transient receptor potential (TRPC) 1 proteins in vascular smooth muscle cells (VSMCs), which contribute to important cellular functions. We have previously shown that PKC is obligatory for activation of TRPC1 SOCs in VSMCs, and the present study investigates if the classic phosphoinositol signaling pathway involving Gaq-mediated PLC activity is responsible for driving PKC-dependent channel gating. The G-protein inhibitor GDP beta-S, anti-Gaq antibodies, the PLC inhibitor U73122, and the PKC inhibitor GF109203X all inhibited activation of TRPC1 SOCs, and U73122 and GF109203X also reduced storeoperated PKC-dependent phosphorylation of TRPC1 proteins. Three distinct SR Ca2+ store-depleting agents, 1,2-bis(2-aminophenoxy) ethane-N, N, N', N'-tetraacetic acid acetoxymethyl ester, cyclopiazonic acid, and N, N, N', N'-tetrakis(2-pyridylmethyl) ethane-1,2-diamineed, induced-translocations of the fluorescent biosensor GFP-PLCd1-PH from the cell membrane to the cytosol, which were inhibited by U73122. Knockdown of PLC beta 1 with small hairpin RNA reduced both store-operated PLC activity and stimulation of TRPC1 SOCs. Immunoprecipitation studies and proximity ligation assays revealed that store depletion induced interactions between TRPC1 and Gaq, and TRPC1 and PLC beta 1. We propose a novel activation mechanism for TRPC1 SOCs in VSMCs, in which store depletion induces formation of TRPC1-Gaq-PLC beta 1 complexes that lead to PKC stimulation and channel gating. C1 [Shi, Jian; Miralles, Francesc; Large, William A.; Albert, Anthony P.] Univ London, Inst Cardiovasc & Cell Sci, Vasc Biol Res Ctr, Cranmer Terrace, London SW17 0RE, England. [Miralles, Francesc] Univ London, Inst Med & Biomed Educ, London, England. [Birnbaumer, Lutz] Natl Inst Environm Hlth Sci, Neurobiol Lab, Res Triangle Pk, NC USA. RP Shi, J (reprint author), Univ London, Inst Cardiovasc & Cell Sci, Vasc Biol Res Ctr, Cranmer Terrace, London SW17 0RE, England. EM jshi@sgul.ac.uk FU Biotechnology and Biological Sciences Research Council (Swindon, United Kingdom) [BB/J007226/1, BB/M018350/1]; U.S. National Institutes of Health Intramural Research Program [Z01-ES-101684] FX The authors thank Dr. Elena Sviderskaya for assistance with electroporation. This work was supported by the Biotechnology and Biological Sciences Research Council (Swindon, United Kingdom) (BB/J007226/1 and BB/M018350/1 to A.P.A.) and was also supported in part by the U.S. National Institutes of Health Intramural Research Program (Project Z01-ES-101684 to L.B.). NR 53 TC 3 Z9 4 U1 3 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD FEB PY 2016 VL 30 IS 2 BP 702 EP 715 DI 10.1096/fj.15-280271 PG 14 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA DC7IA UT WOS:000369391900021 PM 26467792 ER PT J AU Bellinger, BJ Hoffman, JC Angradi, TR Bolgrien, DW Starry, M Elonen, C Jicha, TM Lehto, LP Seifert-Monson, LR Pearson, MS Anderson, L Hill, BH AF Bellinger, Brent J. Hoffman, Joel C. Angradi, Theodore R. Bolgrien, David W. Starry, Matthew Elonen, Colleen Jicha, Terri M. Lehto, LaRae P. Seifert-Monson, Lindsey R. Pearson, Mark S. Anderson, Leroy Hill, Brian H. TI Water quality in the St. Louis River Area of Concern, Lake Superior: Historical and current conditions and delisting implications SO JOURNAL OF GREAT LAKES RESEARCH LA English DT Article DE Beneficial use impairment; Great Lakes; Assessment; St. Louis River AOC; Water quality ID GREAT-LAKES; CHLOROPHYLL-A; TRENDS; RESOURCES; SEDIMENT; ESTUARY; LOADS AB Water quality in the St. Louis River Area of Concern (AOC) was assessed at two stations over a 60 year period (1953-2013) and system-wide for 2012-2013 to determine if the AOC beneficial use impairment (BUI) of "Excessive loading of sediment and nutrients" should be considered for removal. Based on the time-series analysis, concentration and loading of total suspended solids and total phosphorus to Lake Superior from the St. Louis River have decreased over time, and episodic hypoxia in the mainstem of the estuary was eliminated after 1975. Detection of temporal patterns in nitrogen concentration and loading, particularly in the lower estuary, were complicated by Lake Superior nitrogen inputs and changes in wastewater treatment practices. For the system-wide assessment, sample locations were based on a probabilistic survey design. In 2012 and 2013, there was significant monthly (May-October) variability in water quality constituents. Based on area weighted estimates, 60-85% of the estuary surface area was below BUI criterion for total phosphorus, total suspended solids, and chlorophyll a. Water quality in the western arm of Lake Superior in 2013 was indicative of oligotrophic conditions, satisfying delisting requirements. The long-term improvements in water quality followed improvements in watershed land-use practices and treatment of wastewater. The stratified system-wide survey provided unbiased estimates of spatial and temporal condition and identified some outlier sites. The data from this study supports the BUI removal process for the St Louis River AOC. Published by Elsevier B.V. on behalf of International Association for Great Lakes Research. C1 [Bellinger, Brent J.; Hoffman, Joel C.; Angradi, Theodore R.; Bolgrien, David W.; Elonen, Colleen; Jicha, Terri M.; Lehto, LaRae P.; Pearson, Mark S.; Anderson, Leroy; Hill, Brian H.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Midcontinent Ecol Div, 6201 Congdon Blvd, Duluth, MN 55804 USA. [Starry, Matthew] SRA Int, Fairfax, VA 22033 USA. [Seifert-Monson, Lindsey R.] Univ Minnesota, Grad Program Integrat Biosci, Duluth, MN 55812 USA. [Bellinger, Brent J.] City Austin, Watershed Protect Dept, 505 Barton Springs Rd 11th Floor, Austin, TX 78704 USA. [Starry, Matthew] Super Water Light & Power Co, 2915 Hill Ave, Superior, WI 54880 USA. [Lehto, LaRae P.] AMI Consulting Engineers PA, 91 Main St, Superior, WI 54880 USA. [Seifert-Monson, Lindsey R.] Sappi Fine Paper, 20 North 22nd St, Cloquet, MN 55720 USA. RP Bellinger, BJ (reprint author), City Austin, Watershed Protect Dept, 505 Barton Springs Rd 11th Floor, Austin, TX 78704 USA. EM brent.bellinger@austintexas.gov NR 38 TC 1 Z9 1 U1 0 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0380-1330 J9 J GREAT LAKES RES JI J. Gt. Lakes Res. PD FEB PY 2016 VL 42 IS 1 BP 28 EP 38 DI 10.1016/j.jglr.2015.11.008 PG 11 WC Environmental Sciences; Limnology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DD7PB UT WOS:000370115500004 ER EF