FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Huang, BX Kim, HY AF Huang, Bill X. Kim, Hee-Yong TI The role of phosphatidylserine in Akt activation: a novel activation scheme for Akt SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAAA, NIH, LMS, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A980 EP A981 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702050 ER PT J AU Iacono, D O'Brien, R Resnick, S Zonderman, A Pletnikova, O Rudow, G Crain, B Troncoso, J AF Iacono, Diego O'Brien, Richard Resnick, Susan Zonderman, Alan Pletnikova, Olga Rudow, Gay Crain, Barbara Troncoso, Juan TI Differential nuclear and nucleolar hypertrophy of anterior and posterior cingulate neurons in asymptornatic subjects with AD pathology SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Johns Hopkins Univ, Dept Pathol, Div Neuropathol, Baltimore, MD 21205 USA. Johns Hopkins Univ, Dept Neurol, Baltimore, MD 21224 USA. NIA, Lab Personal & Cognit, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A19 EP A19 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500091 ER PT J AU Idelman, G De Siervi, A Haggerty, CM Montano, I Taub, DD Gardner, K AF Idelman, Gila De Siervi, Adriana Haggerty, Cynthia M. Montano, Idalia Taub, Dennis D. Gardner, Kevin TI The role of IGF system in lymphocytes activation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, Bethesda, MD 20892 USA. NIH, Gerontol Res Ctr, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A252 EP A253 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502058 ER PT J AU Igarashi, M DeMar, JC Ma, K Chang, L Bell, JM Rapoport, SI AF Igarashi, Miki DeMar, James C., Jr. Ma, Kaizong Chang, Lisa Bell, Jane M. Rapoport, Stanley I. TI The conversion rate to docosahexaenoic acid from alpha-linolenic acid in rat liver: Effects of n-3 PUFA deprivation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIA, NIH, BPMS, Bethesda, MD 20892 USA. NIAAA, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A339 EP A339 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502475 ER PT J AU Irarrazabal, CE Burg, MB Schentz, M Ferraris, JD AF Irarrazabal, Carlos Ernesto Burg, Maurice B. Schentz, Mike Ferraris, Joan D. TI Contribution of PLC-gamma 1 to activation by high NaCl of the osmoprotective transcription factor TonEBP/OREBP SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NHLBI, NIH, LKEM, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A962 EP A962 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708701522 ER PT J AU Isenberg, JS Romeo, M Abu-Asab, M Tsokas, M Frazier, WA Roberts, DD AF Isenberg, Jeffrey S. Romeo, Martin Abu-Asab, Mores Tsokas, Maria Frazier, William A. Roberts, David D. TI Increased ischemic tissue survival through targeting thrombospondin-1 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA. RI Roberts, David/A-9699-2008 OI Roberts, David/0000-0002-2481-2981 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A11 EP A11 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500053 ER PT J AU Ivanov, AI Bachar, M Babbin, B Adelstein, RS Nusrat, A Parkos, CA AF Ivanov, Andrei I. Bachar, Moshe Babbin, Brian Adelstein, Robert S. Nusrat, Asma Parkos, Charles A. TI A unique role for the nonmuscle myosin IIA in regulation of epithelial apical junctions SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Emory Univ, Pathol & Lab Med, Atlanta, GA 30322 USA. NHLBI, NIH, Mol Cardiol Lab, Bethesda, MD 20892 USA. RI Nusrat, Asma/B-3887-2009; Parkos, Charles/B-3896-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A763 EP A763 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708700137 ER PT J AU Jacob, VA Pisitkun, T Yu, MJ Knepper, MA AF Jacob, Vinitha Anne Pisitkun, T. Yu, M. J. Knepper, M. A. TI Vasopressin-induced phosphorylation of PKB/Akt in the rat inner medullary collecting duct SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A905 EP A905 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708701258 ER PT J AU Jayanthi, S Buie, S Better, W Herning, R Cadet, JL AF Jayanthi, Subramaniam Buie, Stephanie Better, Warren Herning, Ron Cadet, Jean Lud TI Identification of putative biomarkers in the serum of marijuana users by surface-enhanced laser Desorption/Ionization time of flight mass spectrometry (SELDI-TOF-MS) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Natl Inst Drug Abuse, Mol Neuropsychiat Branch, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A421 EP A421 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708503328 ER PT J AU Jeang, KT Chi, YH AF Jeang, Kuan-Teh Chi, Ya-Hui TI Proclivity for constitutive tumors in mice deficient for MAD1 mitotic checkpoint protein SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAID, LMM, Bethesda, MD 20892 USA. RI Jeang, Kuan-Teh/A-2424-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1029 EP A1029 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702293 ER PT J AU Joshi, MB Owings, JP Baer, JA Dwyer, DM AF Joshi, Manju B. Owings, Joshua P. Baer, J. Austin Dwyer, Dennis M. TI Molecular and functional analyses of a novel secretory nuclease from the human pathogen Leishmania donovani SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAID, NIH, Parasit Dis Lab, Cell Biol Sect, Bethesda, MD 20892 USA. NR 0 TC 4 Z9 4 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A277 EP A277 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502175 ER PT J AU Justinova, Z Bortolato, M Mangieri, R Mukhin, AG Chefer, SI Piomelli, D Goldberg, SR AF Justinova, Zuzana Bortolato, Marco Mangieri, Regina Mukhin, Alexey G. Chefer, Svetlana I. Piomelli, Daniele Goldberg, Steven R. TI Lack of abuse liability of the FAAH inhibitor URB597 in squirrel monkeys SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDA, IRP, NIH, Preclin Pharmacol Sect, Baltimore, MD 21224 USA. NIDA, IRP, NIH, Neuroimaging Res Branch, Baltimore, MD 21224 USA. Univ Maryland, Sch Med, MPRC, Baltimore, MD 21228 USA. Univ Calif Irvine, Dept Pharmacol, Irvine, CA 92697 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A409 EP A409 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708503275 ER PT J AU Katz, JL Newman, AH Campbell, B AF Katz, Jonathan L. Newman, Amy H. Campbell, Bettye TI Effects of drugs on visual stimulus control of behavior in an analog of a drug-discrimination procedure SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDA, Intramural Res Program, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A782 EP A782 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708700226 ER PT J AU Khan, FA Nilsson, R Tang, Y Degerman, E Manganiello, VC AF Khan, Faiyaz Ahmad Nilsson, Rebecka Tang, Yan Degerman, Eva Manganiello, Vincent C. TI Insulin-induced formation of macromolecular complexes involved in activation of Phosphodiesterase3B (PDE3B) in 3T3-L1 adipocytes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NHLBI, NIH, PCCMB, Bethesda, MD 20892 USA. Lund Univ, Dept Expt Med Sci, S-22184 Lund, Sweden. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A997 EP A997 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702130 ER PT J AU Kiefman, R Rifkind, JA Fabry, ME Bhattacharya, J AF Kiefman, Rainer Rifkind, Joseph A. Fabry, Mary E. Bhattacharya, Jahar TI Red blood cells induce lung inflammation in hypoxia SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Columbia Univ, New York, NY 10019 USA. Natl Inst Aging, Baltimore, MD 21202 USA. Albert Einstein Coll Med, Div Hematol, Bronx, NY 10461 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1204 EP A1204 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703537 ER PT J AU Kim, SM Huang, YN Faulliaber-Walter, R Mizel, D Wall, SM Briggs, JP Schuermann, J AF Kim, Soo Mi Huang, Yuning Faulliaber-Walter, Robert Mizel, Diane Wall, Susan M. Briggs, Josie P. Schuermann, Jurgen TI Telemetrie blood pressure studies in NKCC1-deficient mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDDK, NIH, Bethesda, MD 20892 USA. Emory Univ, Sch Med, Div Renal, Atlanta, GA 30322 USA. Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A503 EP A503 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708504166 ER PT J AU Kim, SM Hirai, H Cai, T Chen, LM Faulhaber-Walter, R Huang, YN Mizel, D Briggs, JP Notkins, AL Schnermann, J AF Kim, Soo Mi Hirai, Hiroki Cai, Tan Chen, Limeng Faulhaber-Walter, Robert Huang, Yuning Mizel, Diane Briggs, Josie P. Notkins, Abner L. Schnermann, Jurgen TI Dense core vesicle proteins IA-2 and IA-2beta - novel regulators of renin secretion and circadian blood pressure rhythms SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDDK, NIH, Bethesda, MD 20892 USA. NIDCR, NIH, Bethesda, MD 20892 USA. Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1250 EP A1250 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708704204 ER PT J AU Kim, YS Kang, KR Wolff, EC Park, MH AF Kim, Yeon Sook Kang, Kee Ryeon Wolff, Edith C. Park, Myung Hee TI Substrate specificity of deoxyhypusine hydroxylase SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NIDCR, OPCB, Bethesda, MD 20892 USA. Gyeongsang Natl Univ, Dept Chem, Jinju 660751, South Korea. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1015 EP A1015 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702220 ER PT J AU Kim, YH Pech, V Spencer, KB Beierwaltes, WH Everett, LA Green, ED Shin, W Sutliff, RL Wall, SM AF Kim, Young-Hee Pech, Vladimir Spencer, Kathryn B. Beierwaltes, William H. Everett, Lorraine A. Green, Eric D. Shin, Wonkyong Sutliff, Roy L. Wall, Susan M. TI Pendrin, encoded by Slc26 alpha 4, modulates ENaC expression and function SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Emory Univ, Div Renal, Atlanta, GA 30322 USA. Wayne State Univ, Henry Ford Hosp, Sch Med, Hypertens & Vasc Res Div, Detroit, MI 48202 USA. Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England. NHGRI, NIH, Bethesda, MD 20892 USA. Emory Univ, Atlanta, GA 30033 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A902 EP A902 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708701241 ER PT J AU Kimura, T Yeliseev, AA Rhodes, SD Gawrisch, K AF Kimura, Tomohiro Yeliseev, Alexei A. Rhodes, Steven D. Gawrisch, Klaus TI Reconstitution of human peripheral cannabinoid receptor CB2 into phosphatidylcholine proteoliposomes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAAA, NIH, Rockville, MD 20852 USA. RI Yeliseev, Alexei/B-3143-2009 NR 0 TC 0 Z9 0 U1 2 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A983 EP A983 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702061 ER PT J AU Kimura, T Moaddel, R Wainer, IW AF Kimura, Tomoko Moaddel, Ruin Wainer, Irving W. TI Development and characterization of the organic anion transporter column for on line studies of drug-transporter affinities SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIA, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1188 EP A1188 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703465 ER PT J AU Kloda, JH Hines, K Mitchell, DC AF Kloda, Jessica Holden Hines, Kirk Mitchell, Drake C. TI Mechanism of free fatty acid modulation of inhibitory ligand gated ion channels SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAAA, NIH, Lab Membrane Biochem & Biophys, Sect Fluorescence Studies, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A241 EP A241 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502005 ER PT J AU Kobrinsky, E Thomas, S Masoudieh, A Mager, DA Abernethy, DR Soldatov, NM AF Kobrinsky, E. Thomas, S. Masoudieh, A. Mager, D. A. Abernethy, D. R. Soldatov, N. M. TI Heterogeneous signaling pattern of PKC activity associated with the Ca(v)1.2 calcium channel current SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIA, Gerontol Res Ctr, Baltimore, MD 21224 USA. SUNY Buffalo, Dept Pharmaceut Sci, Buffalo, NY 14260 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1155 EP A1155 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703311 ER PT J AU Kohler, JJ Hosseini, S Haase, C Ludaway, T Russ, R Chan, S Copeland, W Lewis, W AF Kohler, James John Hosseini, Seyed Haase, Chad Ludaway, Tomika Russ, Rodney Chan, Sherine Copeland, William Lewis, William TI Genetic changes in human mutant polymerase gamma (Pol [gamma]) alters mtDNA biogenetics and impacts cardiac muscle function SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Emory Univ, Atlanta, GA 30322 USA. NIH, Natl Inst Environm Hlth Sci, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A129 EP A129 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501048 ER PT J AU Kommineni, VK Nagineni, CN Detrick, B Hooks, JJ AF Kommineni, Vijaya Krishna Nagineni, Chandra N. Detrick, Barbara Hooks, John J. TI Inflammatory mediators induce VEGF-A and VEGF-C secretion by human retinal cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NEI, Immunol Lab, Bethesda, MD 20892 USA. Johns Hopkins Univ, Dept Pathol, Baltimore, MD 21205 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A764 EP A764 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708700141 ER PT J AU Kostic, TS Janjic, MM Stojkov, NJ Stojilkovic, SS Andric, SA AF Kostic, Taljana S. Janjic, Marija M. Stojkov, Natasa J. Stojilkovic, Stanko S. Andric, Silvana A. TI Protein kinase G-dependent stimulation of Leydig cell steroidogenesis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Fac Sci, Novi Sad 21000, Serbia. NIH, NICHD, ERRD, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A622 EP A622 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505124 ER PT J AU Krasnova, IN Hodges, AB Ladenheim, B Rhoades, R Desir, N Ogbonna, EI Huntley, P Deng, XL Hohmann, CF Cadet, JL AF Krasnova, Irina N. Hodges, Amber B. Ladenheim, Bruce Rhoades, Raina Desir, Nadia Ogbonna, Eziaku I. Huntley, Patrice Deng, Xiaolin Hohmann, Christine F. Cadet, Jean L. TI Neurotoxic doses of methamphetamine cause neurocognitive abnormalities in mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDA, NIH, DHHS, Mol Neuropsychiat Branch, Baltimore, MD 21224 USA. Morgan State Univ, Dept Biol, Baltimore, MD 21251 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1174 EP A1175 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703399 ER PT J AU Kunin, M Simons, B Irarrazabal, C Shen, RF Wang, GH Burg, MB Ferraris, JD AF Kunin, Margarita Simons, Brigitte Irarrazabal, Carlos Shen, Rong-Fong Wang, Guanghui Burg, Maurice B. Ferraris, Joan D. TI LC-MS/MS identification of phosphorylation sites of transcription factor, tonicity-responsive enhancer/osmotic response element-binding protein (TonEBP/OREBP) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A962 EP A962 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708701524 ER PT J AU Labunskyy, VM Fomenko, DE Shrimali, RK Hatfield, DL Gladyshev, VN AF Labunskyy, Vyacheslav M. Fomenko, Dmitri E. Shrimali, Rajeev K. Hatfield, Dolph L. Gladyshev, Vadim N. TI Expression of Sep15, a thioredoxin-like selenoprotein, is induced in response to accumulation of unfolded proteins in the endoplasmic reticulum SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Nebraska, Dept Biochem, Lincoln, NE 68583 USA. NCI, NIH, Lab Canc Prevent, Bethesda, MD 20892 USA. RI Gladyshev, Vadim/A-9894-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A115 EP A115 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500549 ER PT J AU LaCroix, C Freeling, J Giles, A Wess, J Li, YF AF LaCroix, Carly Freeling, jessica Giles, Alese Wess, Jurgen Li, Yi-Fan TI M2-AchR knockout exhibits deteriorated cardiac dysfunction and remodeling SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ S Dakota, Sanford Med Sch, Vermillion, SD 57069 USA. NIDDK, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1263 EP A1263 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708704259 ER PT J AU Lee, SK Kim, YS Lee, SS Lee, YJ Lee, YW Park, SC Chi, JG AF Lee, Suk Keun Kim, Yeon Sock Lee, Sang Shin Lee, Young Joon Lee, Yun Woo Park, Sang Chul Chi, Je Geun TI Prediction of genomic DNA mutation by DNA base pair polarity program SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC SP Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 Kangnung Natl Univ, Coll Dent, Kangnung 210702, South Korea. NIDCR, NIH, Bethesda, MD 20892 USA. Seoul Natl Univ, Coll Med, Seoul 110990, South Korea. RI Chi, Je Geun/G-4989-2011 NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A658 EP A658 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505303 ER PT J AU Li, JH Han, SJ Hamdan, FF Kim, SK Jacobson, KA Bloodworth, LM Zhang, XH Wess, J AF Li, Jian H. Han, Sung-Jun Hamdan, Fadi F. Kim, Soo-Kyung Jacobson, Kenneth A. Bloodworth, Lanh M. Zhang, Xiaohong Wess, Jurgen TI Identification of distinct, ligand-specilic structural changes in a G protein-coupled receptor SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDDK, Lab Bioorgan Chem, Mol Signalling Sec, Bethesda, MD 20892 USA. NIH, NIDDK, Lab Bioorgan Chem, Mol Recognit Sec, Bethesda, MD 20892 USA. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A425 EP A425 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708503351 ER PT J AU Li, JX Rice, K France, CP AF Li, Jun-Xu Rice, Kenner France, Charles P. TI Behavioral effects of dipropyltryptamine (DPT) in rats: role of 5-HT1A and. 5-HT2A receptors SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Texas, Hlth Sci Ctr, Dept Pharmacol, San Antonio, TX 78229 USA. NIDDK, NIH, Med Chem Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A780 EP A780 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708700217 ER PT J AU Li, LB Kopajtic, TA Desai, RI Cyriac, GC Newman, AH Katz, JL AF Li, Li-Bin Kopajtic, Theresa A. Desai, Rajeev I. Cyriac, George C. Newman, Amy H. Katz, Jonathan L. TI Subjective effect of monoamine uptake inhibitors in rats trained to discriminate citalopram SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDA, Intramural Res Program, Medications Discovery Res Branch, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A779 EP A779 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708700211 ER PT J AU Lin, YH Brown, JA Fedorova, I Chedester, L Salem, N AF Lin, Yu Hong Brown, James A. Fedorova, Irina Chedester, Lee Salem, Norman, Jr. TI Differential responses of male and female rats: Composition and accumulation of essential fatty acids in plasma SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAAA, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A665 EP A665 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505339 ER PT J AU Liu, DM Metter, EJ Ferrucci, L Roth, SM AF Liu, Dongmei Metter, E. Jeffrey Ferrucci, Luigi Roth, Stephen M. TI TNF alpha-308G/A genotype is associated with muscle mass in humans SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Maryland, College Pk, MD 20742 USA. NIA, Harbor Hosp, Clin Res Branch, Baltimore, MD 21224 USA. RI Liu, Dongmei/C-1525-2012 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1308 EP A1309 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708704472 ER PT J AU Liu, XH Hamelink, C Eiden, LE AF Liu, Xiuhuai Hamelink, Carol Eiden, Lee E. TI The tissue specifier element (TSE) functions as a Ca2+response element for Ca2+and cAMP synergistic signaling to the human vasoactive intestinal polypeptide (VIP) gene SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIMH, Mol Neurosci Sect, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1035 EP A1035 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702322 ER PT J AU Lobanov, A Castellano, S Hatfield, DL Gladyshev, VN AF Lobanov, Alexey Castellano, Sergi Hatfield, Dolph L. Gladyshev, Vadim N. TI Reduced utilization of selenium during evolution of mammals SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Nebraska, Beadle Ctr, Lincoln, NE 68588 USA. HHMI, Janelia Farm, Ashburn, VA 20147 USA. NIH, NCI, Mol Biol Selenium Sect, Bethesda, MD 20892 USA. RI Gladyshev, Vadim/A-9894-2013 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A105 EP A106 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500507 ER PT J AU Londono, D Marques, A Cadavid, D AF Londono, Diana Marques, Adriana Cadavid, Diego TI IL-10 protects the cerebral microcirculation from spirochetal injury SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Med & Dent New Jersey, Newark, NJ 07103 USA. NIAID, NIH, Lab Clin Infect Dis, Clin Studies Unit, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A66 EP A66 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500314 ER PT J AU Lynn, EG McLeod, CJ Sack, MN AF Lynn, Edward G. McLeod, Christopher J. Sack, Michael N. TI Genetic depletion of SirT2 augments cell survival after hypoxia-reoxygenation injury SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NHLBI, NIH, Cardiol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A666 EP A666 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505345 ER PT J AU Ma, XC Chen, C Shah, Y Morimura, K Krausz, KW Idle, JR Gonzalez, FJ AF Ma, Xiaochao Chen, Chi Shah, Yatrik Morimura, Keiichirou Krausz, Kristopher W. Idle, Jeffrey R. Gonzalez, Frank J. TI The effect of pregnane X receptor (PXR)-mediated CYP3A induction on acetaminophen hepatotoxicity SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, NIH, Bethesda, MD 20892 USA. Charles Univ Prague, Inst Pharmacol, Prague, Czech Republic. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1184 EP A1184 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703446 ER PT J AU Manjunatha, UH AF Manjunatha, Ujjini Havaldar TI The mechanism of action of 4-nitroimidazoles against mycobacterium tuberculosis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAID, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A207 EP A207 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501421 ER PT J AU Marino-Ramirez, L Jordan, IK Landsman, D AF Marino-Ramirez, Leonardo Jordan, I. King Landsman, David TI Multiple evolutionary solutions to core histone gene regulation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. Georgia Tech Univ, Sch Biol, Atlanta, GA 30332 USA. RI Landsman, David/C-5923-2009; Marino-Ramirez, Leonardo/I-5759-2013 OI Marino-Ramirez, Leonardo/0000-0002-5716-8512 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1033 EP A1033 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702312 ER PT J AU Martin-Manso, G Mosher, DF Roberts, DD AF Martin-Manso, Gema Mosher, Deane F. Roberts, David D. TI The N-terminal module of, thrombospondin-I enhances superoxide generation from differentiated U937 human monocytic cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, Bethesda, MD 20892 USA. Univ Wisconsin, Madison, WI 53706 USA. RI Roberts, David/A-9699-2008; Martin Manso, Maria Gema/D-4612-2013 OI Roberts, David/0000-0002-2481-2981; NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1146 EP A1146 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703268 ER PT J AU Masood, AM Salem, N AF Masood, Athar M. Salem, Norman, Jr. TI High throughput fatty acid analysis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, LMBB, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A265 EP A265 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502119 ER PT J AU McGugan, GC Joshi, MB Dwyer, DM AF McGugan, Glen Chapman, Jr. Joshi, Manju B. Dwyer, Dennis M. TI Expression and functional characterization of endosomal nucleases of the human enteric pathogen, Entamoeba histolytica SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAID, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A277 EP A277 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502174 ER PT J AU Miller, DS Hartz, AMS Bauer, J AF Miller, David S. Hartz, Anika M. S. Bauer, Joern TI Signals modulating p-Glycoprotein activity in brain capillaries SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NIEHS, Chem Pharmacol Lab, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A874 EP A874 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708701112 ER PT J AU Moaddel, R Ravichandran, S Bigi, F Collins, J Yamaguchi, R Wainer, IW AF Moaddel, Ruin Ravichandran, Sarangan Bigi, Federica Collins, Jack Yamaguchi, Rika Wainer, Irving W. TI Pharmacophore modeling of non transported competitive inhibitors of the human Organic Cation Transporter, hOCT1 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIA, Gerontol Res Ctr, NIH, Baltimore, MD 20874 USA. NCI, ABCC, NIH, Frederick, MD 21701 USA. NIA, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1187 EP A1187 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703459 ER PT J AU Moody, TW Berna, M Mantey, S Jensen, R AF Moody, Terry W. Berna, Marc Mantey, Samuel Jensen, Robert TI Neuromedin B causes transactivation of Epidermal Growth Factor receptors in lung cancer cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, CCR, OD, Bethesda, MD 20892 USA. NIDDK, DDB, Bethesda, MD 20892 USA. NIDDK, DDB, HHS, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1150 EP A1150 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703286 ER PT J AU Moon, KH Hood, BL Mukhopadhyay, P Conrads, TP Veenstra, TD Song, BJ Pacher, P AF Moon, Kwan-Hoon Hood, Brian L. Mukhopadhyay, Partha Conrads, Thomas P. Veenstra, Timothy D. Song, Byoung J. Pacher, Pal TI Identification of oxidized and S-nitrosylated mitochondrial proteins in hepatic ischemia-reperfusion injury SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAAA, NIH, Rockville, MD 20852 USA. SAIC Frederick Inc, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A662 EP A662 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505323 ER PT J AU Mukhopadhyay, B Shakoury-Elizeh, M Philpott, C AF Mukhopadhyay, Bani Shakoury-Elizeh, Minoo Philpott, Caroline TI Identification of Uga3 as an iron-regulated transcription factor controlling the activity of the GABA shunt in Saccharomyces cerevisiae SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDDK, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1042 EP A1042 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702357 ER PT J AU Mukoyama, Y AF Mukoyama, Yosuke TI Nerve-vessel interactions during vertebrate development SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A197 EP A197 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501370 ER PT J AU Mustafa, T Walsh, J Grimaldi, M Eiden, L AF Mustafa, Tomris Walsh, James Grimaldi, Maurizio Eiden, Lee TI The hop domain of the PAC1 receptor confers coupling to intracellular Ca2+ elevation required for PACAP-evoked catecholamine secretion SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIMH, Sect Mol Sci, Bethesda, MD 20892 USA. Southern Res Inst, Drug Discovery Div, Birmingham, AL 35205 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A982 EP A982 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702058 ER PT J AU Nagineni, CN Raju, R Kommineni, VK Detrick, B Hooks, JJ AF Nagineni, Chandra N. Raju, Raghavan Kommineni, Vijaya Krishna Detrick, Barbara Hooks, John J. TI Expression of extracellular matrix protein anosmin (KAL-1) in human retinal cells is upregulated by TGF-beta SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NEI, Immunol Lab, Bethesda, MD 20892 USA. Univ Alabama, Birmingham, AL 35294 USA. Johns Hopkins Univ, Baltimore, MD 21205 USA. RI Raju, Raghavan/E-9219-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1154 EP A1154 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703306 ER PT J AU Nekhai, S Ammosova, T Charles, S Jeang, KT AF Nekhai, Sergei Ammosova, Tatyana Charles, Sharroya Jeang, Kuan-Teh TI Regulation of HIV-1 transcription by protein phosphatase 1 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Howard Univ, Ctr Sickle Cell Dis, Washington, DC 20059 USA. Howard Univ, Washington, DC 20059 USA. NIAID, NIH, Mol Microbiol Lab, Mol Virol Sect, Bethesda, MD 20892 USA. RI Jeang, Kuan-Teh/A-2424-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1033 EP A1033 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702313 ER PT J AU Neutzner, A Karbowski, M Youle, RJ AF Neutzner, Albert Karbowski, Mariusz Youle, Richard J. TI Mitochondrial morphology is regulated by RING domain proteins. SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NINDS, NIH, SNB, Bethesda, MD 20892 USA. Univ Maryland, Inst Biotechnol, Ctr Med Biotechnol, Baltimore, MD 21201 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A661 EP A661 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505319 ER PT J AU Newby, PK Maras, J Bakun, P Muller, D Ferrucci, L Tucker, KL AF Newby, P. K. Maras, Janice Bakun, Peter Muller, Denis Ferrucci, Luigi Tucker, Katherine L. TI Whole grains, refined grains, and cereal fiber measured using 7-d diet records: associations with risk factors for chronic disease SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NIA, NIH, Baltimore, MD 21225 USA. RI Tucker, Katherine/A-4545-2010 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A177 EP A177 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501278 ER PT J AU Ni, W Murphy, DL Watts, SW AF Ni, Wei Murphy, Dennis L. Watts, Stephanie W. TI Existence of multiple 5-HT uptake. mechanisms in peripheral arteries SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Michigan State Univ, E Lansing, MI 48824 USA. NIMH, Clin Sci Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A518 EP A518 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708504238 ER PT J AU Niu, SL Culyba, E Mitchell, DC AF Niu, Shui-Lin Culyba, Elizabeth Mitchell, Drake C. TI Characterization of polyunsaturated fatty acid binding to albumin using isothermal titration calorimetry SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAAA, NIH, LMBB, Rockville, MD 20852 USA. Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A981 EP A981 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702051 ER PT J AU Notari, L Arakaki, N Amaral, J Mueller, D Yergey, A Becerra, SP AF Notari, Luigi Arakaki, Naokatu Amaral, Juan Mueller, David Yergey, Alfred Becerra, S. Patricia TI Interactions between pigment epithelium-derived factor (PEDF) and F-1/F-0 ATP synthase provide insights into its antiangiogenic mechanism SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NEI, NIH, Sect Prot Struct & Funct, Bethesda, MD 20892 USA. Rosalind Franklin Univ Med, N Chicago, IL 60064 USA. NICHD, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A249 EP A249 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502040 ER PT J AU Ono, T Kasamatsu, A Oka, S Moss, J AF Ono, Tohru Kasamatsu, Atsushi Oka, Shunya Moss, Joel TI The 39-kDa poly(ADP-ribose) glycohydrolase, ARH3, hydrolyzes O-Acetyl-ADP-ribose, a product of the Sir2 family of acetyl-histone deacetylases. SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NHLBI, NIH, P CCMB, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A641 EP A641 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505223 ER PT J AU Oppermann, M Faulhaber-Walter, R Castrop, H Mizel, D Heilig, K Heilig, CW Schnermann, J AF Oppermann, Mona Faulhaber-Walter, Robert Castrop, Hayo Mizel, Diane Heilig, Kathleen Heilig, Charles W. Schnermann, Jurgen TI Glomerular hypertension and hyperfiltration in young fvb.ROP Os/+ mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDDK, NIH, Bethesda, MD 20892 USA. Univ Chicago Hosp, Dept Med, Chicago, IL 60637 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A503 EP A503 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708504168 ER PT J AU Orlova, V Economopoulou, M Lupu, F Santoso, S Chavakis, T AF Orlova, Valeria Economopoulou, Matina Lupu, Florea Santoso, Sentot Chavakis, Triantafyllos TI Junctional adhesion molecule (JAM)-C regulates endothelial permeability by modulating VE-cadherin-mediated interendothelial contacts SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, Expt Immunol Branch, Bethesda, MD 20892 USA. NEI, Bethesda, MD 20892 USA. Oklahoma Med Res Fdn, Oklahoma City, OK 73104 USA. Univ Giessen, Giessen, Germany. RI Lupu, Florea/C-3162-2009 OI Lupu, Florea/0000-0003-1249-9278 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A187 EP A187 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501327 ER PT J AU Pan, Y Pratt, C AF Pan, Yang Pratt, Charlotte TI Metabolic syndrome and its association with diet and physical activity in US adolescents SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Maryland, Dept Food Sci & Nutr, College Pk, MD 20742 USA. NHLBI, Div Prevent & Populat Sci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A167 EP A167 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501233 ER PT J AU Pani, B Liu, XB Ambudkar, IS Singh, BB AF Pani, Biswaranjan Liu, Xibao Ambudkar, Indu S. Singh, Brij B. TI Compartmentalization of TRPC1-STIM1 interactions into lipid raft domains SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ N Dakota, Sch Med & Hlth Sci, Grand Forks, ND 58203 USA. NIDCR, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1425 EP A1425 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708705456 ER PT J AU Perry, JL Srimaroeng, C Dembla-Rajpal, N Hall, LA Pritchard, JB AF Perry, Jennifer L. Srimaroeng, Chutima Dembla-Rajpal, Neetu Hall, Laura A. Pritchard, John B. TI Substrate specificity in a model of the human organic anion transporter 3 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIEHS, Lab Pharmacol & Chem, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A908 EP A908 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708701271 ER PT J AU Pfeiffer, CM Johnson, CL Jain, RB Yetley, EA Picciano, MF Rader, JI Fisher, KD Mulinare, J Osterloh, JD AF Pfeiffer, Christine M. Johnson, Clifford L. Jain, Ram B. Yetley, Elizabeth A. Picciano, Mary Frances Rader, Jeanne I. Fisher, Ken D. Mulinare, Joe Osterloh, John D. TI Trends in blood folate levels in the United States, 1988-2004 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Ctr Dis Control & Prevent, Atlanta, GA 30341 USA. Ctr Dis Control & Prevent, HYAT Bldg 4, Hyattsville, MD USA. NIH, Rockville, MD 20892 USA. Food & Drug Adm, CPK1, College Pk, MD USA. Ctr Dis Control & Prevent, EXPK Bldg 12, Atlanta, GA 30329 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A104 EP A104 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500500 ER PT J AU Pillai, VC Bazinet, RP Rapoport, SI Weis, MT AF Pillai, Venkateswaran C. Bazinet, Richard P. Rapoport, Stanley I. Weis, Margaret T. TI Identification and characterisation of substrate requirements for brain long chain fatty acyl CoA synthase (LCAFCoAS) isoforms SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC SP Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 Texas Tech Univ, Hlth Sci Ctr, Amarillo, TX USA. Natl Inst Hlth, Natl Inst Aging, Bethesda, MD 20814 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A607 EP A607 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505052 ER PT J AU Poliakov, E Gentleman, S Redmond, TM AF Poliakov, Eugenia Gentleman, Susan Redmond, T. Michael TI Role of tyrosines in mouse beta-carotene 15,15 '-monooxygenase activity SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NEI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A274 EP A274 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502163 ER PT J AU Ponzio, TA Yue, CM Gainer, H AF Ponzio, Todd A. Yue, Chunmei Gainer, Harold TI Transcriptional activity of the vasopressin gene measured by onestep quantitative RT-PCR. SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NINDS, Neurochem Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1391 EP A1392 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708705303 ER PT J AU Protchenko, O Androphy, R Rodriguez-Suarez, R Bussey, H Protchenko, CC AF Protchenko, Olga Androphy, Rachel Rodriguez-Suarez, Roberto Bussey, Howard Protchenko, Caroline C. TI Identification of FLC family of proteins required for import of FAD into the endoplasmic reticulum in a screen for heme uptake genes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDDK, NIH, Bethesda, MD 20892 USA. McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A244 EP A244 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502018 ER PT J AU Reedy, J Krebs-Smith, SM AF Reedy, Jill Krebs-Smith, Susan M. TI Dietary advice for the general public: conflicting or consistent? SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A305 EP A305 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502314 ER PT J AU Robinson, RA Adams, JP Dudek, SM AF Robinson, Rachel Anne Adams, Jennifer Paige Dudek, Serena M. TI Role of NMDA receptors in long-term potentiation-induced modulation of transcription factor binding SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIEHS, Neurobiol Lab, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A597 EP A597 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505005 ER PT J AU Ronel, M Liu, J Blonder, J Veenstra, TD AF Ronel, Malena Liu, Jun Blonder, Josip Veenstra, Timothy D. TI Targeting and insertion of the cholesterol-binding translocator protein into the outer mitochondrial membrane SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Georgetown Univ, Dept Biochem, Washington, DC 20007 USA. Natl Canc Inst, Lab Proteom & Analyt Technol, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A611 EP A612 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505074 ER PT J AU Roseland, JM Andrews, K Zhao, CW Schweitzer, A Holden, J Perry, C Dwyer, J Picciano, MF Fisher, K Saldanha, L Yetley, E Douglass, L AF Roseland, Janet Maxwell Andrews, Karen Zhao, Cuiwei Schweitzer, Amy Holden, Joanne Perry, Charles Dwyer, Johanna Picciano, Mary Frances Fisher, Kenneth Saldanha, Leila Yetley, Elizabeth Douglass, Larry TI Selection of adult multivitamin/mineral products for comprehensive analytical nutrient content study SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC SP Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 USDA ARS, Nutr Data Lab, Beltsville, MD 20705 USA. NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. Univ Maryland, Biometr Program, College Pk, MD 20742 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A309 EP A309 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502336 ER PT J AU Rosemond, E Li, B McMillin, S Wess, J AF Rosemond, Erica Li, Bo McMillin, Sally Wess, Jurgen TI A novel strategy to identify proteins that interact with the M3 muscarinic receptor in vivo using the split-ubiquitin system in yeast SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDDK, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A424 EP A424 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708503344 ER PT J AU Ross, RJ Zhou, M Shen, DF Bojanowski, CM Fariss, R Tuo, JS Chan, CC AF Ross, Robert James Zhou, Min Shen, Defen Bojanowski, Christine M. Fariss, Robert Tuo, Jingsheng Chan, Chi-Chao TI Immunological protein expression in the eyes of Ccl2/Cx3cr1 deficient mice, a model of age-related macular degeneration SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NEI, Immunol Lab, Bethesda, MD 20892 USA. NEI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1130 EP A1130 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703194 ER PT J AU Saha, A Kim, SJ Zhang, ZJ Lee, YC Tsai, PC Mukherjee, AB AF Saha, Arjun Kim, Sung-Jo Zhang, Zhongjian Lee, Yi-Ching Tsai, Pei-Chih Mukherjee, Anil B. TI Elevated expression of S100B and RAGE positively correlates with pro-inflammatory cytokine production that may contributie to rapid neurodegen.eration in INCL SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NICHD, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A989 EP A989 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702093 ER PT J AU Sauna, ZE Kim, IW Nandigama, K Ambudkar, SV AF Sauna, Zuben Erach Kim, In-Wha Nandigama, Krishnamachary Ambudkar, Suresh V. TI ATP-driven dimerization of nucleotide binding domains, asymmetric occlusion of one nucleotide and ADP-driven disassembly of the occluded state are important reaction intermediates of the P-glycoprotein transport cycle SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, NIH, Cell Biol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A242 EP A242 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502008 ER PT J AU Schnermann, J AF Schnermann, Jurgen TI Tubuloglomerular feedback - A system for nephron self-regulation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NIDDK, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A1 EP A2 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500007 ER PT J AU Schoneveld, OJLM Cidlowski, JA AF Schoneveld, Onard Jan L. M. Cidlowski, John A. TI Regulatory effects of glucocorticoid receptor phosphorylation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIEHS, LST, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A253 EP A253 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502063 ER PT J AU Schweitzer, A Andrews, K Roseland, J Zhao, CW Holden, J Perry, C Douglass, L Dwyer, J Picciano, MF Fisher, K Saldanha, L Yetley, E AF Schweitzer, Amy Andrews, Karen Roseland, Janet Zhao, Cuiwei Holden, Joanne Perry, Charles Douglass, Larry Dwyer, Johanna Picciano, Mary Frances Fisher, Kenneth Saldanha, Leila Yetley, Elizabeth TI Variability of four nutrients evaluated in adult multivitamin/mineral (MVM) products SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 USDA ARS, Nutr Data Lab, Beltsville, MD 20705 USA. Univ Maryland, Biometr Program, College Pk, MD 20892 USA. NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A310 EP A310 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502339 ER PT J AU Sengupta, A Carlson, BA Hoffmann, VJ Gladyshev, VN Hatfield, DL AF Sengupta, Aniruddha Carlson, Bradley A. Hoffmann, Victoria J. Gladyshev, Vadim N. Hatfield, Dolph L. TI Conditional knockout of selenocysteine tRNA gene (trsp) in liver modulates lipoprotein metabolism SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, NIH, LCP, CCR, Bethesda, MD 20892 USA. NIH, DIRS, ORS, OD, Bethesda, MD 20892 USA. Univ Nebraska, Dept Biochem, Lincoln, NE 68583 USA. RI Gladyshev, Vadim/A-9894-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A114 EP A114 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500543 ER PT J AU Shea, MK Benjamin, EJ Dupuis, J Massaro, JM Jacques, PF D'Agostino, RB Ordovas, JM O'Donnell, CJ Dawson-Hughes, B Vasan, RS Booth, SL AF Shea, M. Kyla Benjamin, Emelia J. Dupuis, Josee Massaro, Joseph M. Jacques, Paul F. D'Agostino, Ralph B. Ordovas, Jose M. O'Donnell, Christopher J. Dawson-Hughes, Bess Vasan, Ramachandran S. Booth, Sarah L. TI Clinical correlates and heritability of vitamins K and D SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Tufts Univ, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NHLBI, Framingham Heart Study, Framingham, MA 01702 USA. Boston Univ, Sch Med, Boston, MA 02118 USA. Boston Univ, Dept Biostat, Boston, MA 02118 USA. Boston Univ, Dept Math, Boston, MA 02115 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A174 EP A174 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501264 ER PT J AU Shen, KZ Zheng, SS Park, OY Sun, ZL Gao, B AF Shen, Kezhen Zheng, Shu-Sen Park, Ogyi Sun, Zhaoli Gao, Bin TI Liver regeneration after transplantation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAAA, NIH, Rockville, MD 20852 USA. Zhejiang Univ, Affiliated Hosp 1, Coll Med, Hangzhou, Peoples R China. Johns Hopkins Univ, Sch Med, Dept Surg, Baltimore, MD 21287 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1136 EP A1137 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703224 ER PT J AU Shen, WX Manganiello, V AF Shen, Weixing Manganiello, Vincent TI Dysregulation of Cdc25B in PDE3a-/- mice oocytes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A615 EP A615 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505091 ER PT J AU Shi, HF Philpott, C AF Shi, Haifeng Philpott, Caroline TI Identification of genes facilitating iron storage in ferritin SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NIDDK, LDB, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1005 EP A1006 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702174 ER PT J AU Shizukuda, Y Bolan, CD Tripodi, DJ Sachdev, V Nguyen, T Sidenko, S Ernst, I Yau, YY Botello, G Leitman, SF Ali, MI Rosing, DR AF Shizukuda, Yukitaka Bolan, Charles D. Tripodi, Dorothy J. Sachdev, Vandana Nguyen, Tammy Sidenko, Stanislav Ernst, Inez Yau, Yu Ying Botello, Gilberto Leitman, Susan F. Ali, Mir I. Rosing, Douglas R. TI Left atrial contractile function is persistently augmented in newly diagnosed asymptornatic hereditary hemochromatosis subjects despite one year therapy SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NHLBI, NIH, Bethesda, MD 20892 USA. NIH, Dept Transfus Med, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1409 EP A1409 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708705385 ER PT J AU Shrimali, RK Irons, RD Carlson, BA Park, JM Hatfield, DL AF Shrimali, Rajeev Kumar Irons, Robert D. Carlson, Bradley A. Park, Jin M. Hatfield, Dolph L. TI Selenoprotein deficiency adversely affects T cell development, proliferation, TCR signaling and cell mediated immunity SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, NIH, MBSS, CCR,LCP, Bethesda, MD 20892 USA. NCI, NIH, DCP, NSRG, Bethesda, MD USA. Harvard Univ, Sch Med, MGH, CBRC, Charlestown, MA 02129 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A114 EP A114 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500545 ER PT J AU Sieber, JL Krebs-Smith, S Reedy, J AF Sieber, Jessica Lynne Krebs-Smith, Sue Reedy, Jill TI What people are really eating: Food intakes relative to recommendations SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Tennessee, Knoxville, TN 37996 USA. NCI, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A51 EP A51 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500246 ER PT J AU Simons, B Wang, GH Shen, RF Knepper, MA AF Simons, Brigitte Wang, Guanghui Shen, R. -F. Knepper, Mark A. TI Quantitative large-scale phosphotyrosine proteomics of vasopressinsensitive inner medullary collecting duct cells using IVICAT N-terminal labeling and LC-MS/MS SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NHLBI, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1353 EP A1353 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708705124 ER PT J AU Soubias, O Teague, WE Gawrisch, K AF Soubias, Olivier Teague, Walter E. Gawrisch, Klaus TI Experimental evidence for specificity in rhodopsin-lipid interactions SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAAA, NIH, LMBB, Rockville, MD 20852 USA. NR 2 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A980 EP A980 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702047 ER PT J AU Spehalski, E Martin, PL Rozenberg, JM Hoenerhoff, MJ Hoover, SB Walling, BE Vinson, CR Simpson, RM AF Spehalski, Elizabeth Martin, Philip L. Rozenberg, Julian M. Hoenerhoff, Mark J. Hoover, Shelley B. Walling, Brent E. Vinson, Charles R. Simpson, R. M. TI Progression to heart failure modulated in a conditional H-Ras-V12 mouse model of human cardiomyopathy SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Natl Canc Inst, CCR Comparat Mol Pathol Unit, Bethesda, MD 20892 USA. Natl Canc Inst, Lab Metab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A129 EP A129 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501047 ER PT J AU Sperber, S Dawid, I AF Sperber, Steven Dawid, Igor TI Barx1 is necessary for zebrafish cranial neural crest patterning and pharyngeal arch chondrogenesis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NICHD, Mol Genet Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A970 EP A970 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708701565 ER PT J AU Srimaroeng, C Perry, JL Walden, R Pritchard, JB AF Srimaroeng, Chutima Perry, Jennifer L. Walden, Ramsey Pritchard, John B. TI Regulation of organic anion transporter 3 (OAT3) expression and function by membrane lipid raft-associated proteins and cytoskeletons SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIEHS, Lab Pharmacol & Chem, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A907 EP A907 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708701266 ER PT J AU Strader, MB Chen, CY Makusky, AJ Costantino, N Kowalak, JA Court, DL Markey, SP AF Strader, Michael Brad Chen, Cai Yun Makusky, Anthony J. Costantino, Nina Kowalak, Jeffrey A. Court, Donald L. Markey, Sanford P. TI Toward determining the biological function of a novel posttranslational modification on the ribosomal protein S12 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIMH, NIH, Bethesda, MD 20892 USA. NCI, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A266 EP A266 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502122 ER PT J AU Stroth, N Hamelink, C Eiden, LE AF Stroth, Nikolas Hamelink, Carol Eiden, Lee E. TI PACAP-dependent cellular plasticity in the mouse adrenal gland SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIMH, Mol Neurosci Sect, Bethesda, MD 20892 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1249 EP A1250 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708704199 ER PT J AU Sun, JH Steenbergen, C Murphy, E AF Sun, Junhui Steenbergen, Charles Murphy, Elizabeth TI Preconditioning increases S-nitrosylation of l-type calcium channel and SERCA2a SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NHLBI, Bethesda, MD 20892 USA. Johns Hopkins Univ, Dept Pathol, Baltimore, MD 21205 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1379 EP A1379 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708705245 ER PT J AU Takikita, M Hu, N Giffen, C Taylor, PR Hewitt, SM AF Takikita, Mikiko Hu, Nan Giffen, Carol Taylor, Philip R. Hewitt, Stephen M. TI Bcl-2/p-FADD could be a potential prognostic marker in esophageal squamous cell carcinoma SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NCI, CCR, Lab Pathol,Tissue Array Res Program, Bethesda, MD 20892 USA. NIH, NCI, MSC 7236, Canc Prevent Studies Branch, Bethesda, MD 20892 USA. Informat Management Serv Inc, Silver Spring, MD 20904 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A753 EP A753 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708700090 ER PT J AU Tanaka, M Jaruga, P Dizdaroglu, M Chock, PB Stadtman, ER AF Tanaka, Mikiei Jaruga, Pawel Dizdaroglu, Miral Chock, P. Boon Stadtman, Earl R. TI Potential biological consequences of mRNA oxidation-induced translation errors SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NHLBI, LB, Bethesda, MD 20892 USA. NIST, NIST Bldg 227, Gaithersburg, MD 20899 USA. RI Jaruga, Pawel/M-4378-2015 NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1026 EP A1026 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702277 ER PT J AU Thomas, A Kramer, KL AF Thomas, Ajay Kramer, Kenneth L. TI Identifying and characterizing peptides that bind to specific heparan sulfate disaccharides SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NHLBI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1009 EP A1009 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702192 ER PT J AU Thompson, DM Srimaroeng, C Dallas, SL Walden, R Miller, DS Pritchard, JB AF Thompson, Deborah M. Srimaroeng, Chutima Dallas, Shannon L. Walden, Ramsey Miller, David S. Pritchard, John B. TI Functional characterization of human organic anion transporter 4 (hOAT4) in a Baculovirus expression system SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIEHS, Lab Pharmacol & Chem, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A907 EP A907 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708701265 ER PT J AU Tronci, V Tanda, G AF Tronci, Valeria Tanda, Gianluigi TI Involvement of CB1 cannabinoid receptors in cocaine-induced locomotor sensitization after single pre-exposure in mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDA, IRP, NIH, DHHS, Baltimore, MD 21224 USA. RI Tanda, Gianluigi/B-3318-2009 OI Tanda, Gianluigi/0000-0001-9526-9878 NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A410 EP A410 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708503279 ER PT J AU Tsihlis, ND Saavedra, JE Keefer, LK Jiang, Q Kibbe, MR AF Tsihlis, Nick D. Saavedra, Joseph E. Keefer, Larry K. Jiang, Qun Kibbe, Melina R. TI Anti-proliferative properties of IPA/NO: a novel use of an HNO-eluting compound SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Northwestern Univ, Chicago, IL 60611 USA. SAIC Frederick, Natl Canc Inst, BRP, Frederick, MD 21702 USA. LLC CCR, Natl Canc Inst, Frederick, MD 21702 USA. RI Keefer, Larry/N-3247-2014 OI Keefer, Larry/0000-0001-7489-9555 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1129 EP A1129 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703190 ER PT J AU Tu, XY Sarafianos, SG Han, QW Das, K Hou, XR Bauman, J Clark, AD Gaffney, BL Jones, RA Boyer, PL Hughes, SH Arnold, E AF Tu, Xiongying Sarafianos, Stefan G. Han, Qianwei Das, Kalyan Hou, Xiaorong Bauman, Joe Clark, Arthur D. Gaffney, Babara L. Jones, Roger A. Boyer, Paul L. Hughes, Stephen H. Arnold, Eddy TI Structures of wild-type and AZT-Resistant HIV-1 reverse transcriptase complexed with AZTppppA yield insights into the nucleotide excision mechanism SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA. Rutgers State Univ, Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA. NCI, HIV Drug Resistance Program, Frederick, MD 21702 USA. RI Tu, Xiongying/H-7983-2012 NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A640 EP A640 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505214 ER PT J AU Tuo, JS Rosenberg, KI Zhou, M Shen, DF Ross, RJ Faiss, RN Yin, CY Zhuang, ZP Chan, CC AF Tuo, Jingsheng Rosenberg, Kevin I. Zhou, Min Shen, Defen Ross, Robert J. Faiss, Robert N. Yin, Chunyue Zhuang, Zhengping Chan, Chi-Chao TI Aberrant endoplasmic reticulum proteins in Ccl2/Cx3cr1 deficient mice with retinal lesions mimicking human age-related macular degeneration SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NEI, NIH, LI, Bethesda, MD 20892 USA. NEI, NIH, Imagine Core, Bethesda, MD 20892 USA. NINDS, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1130 EP A1130 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703196 ER PT J AU Turanov, A Kehr, S Carlson, BA Hatfield, DL Gladyshev, VN AF Turanov, Anton Kehr, Sebastian Carlson, Bradley A. Hatfield, Dolph L. Gladyshev, Vadim N. TI Mammalian thioredoxin reductases: roles in redox homeostasis and analysis of cellular targets SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Nebraska, Beadle Ctr, Lincoln, NE 68588 USA. NIH, Ctr Canc Res, Bethesda, MD 20892 USA. RI Gladyshev, Vadim/A-9894-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A718 EP A718 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505583 ER PT J AU Turjanski, AG Best, R Hummer, G Gutkind, JS AF Turjanski, Adrian Gustavo Best, Robert Hummer, Gerhard Gutkind, J. Silvio TI The. folding and binding pathway of the transcription factor CREB to CBP SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIDCR, NIH, Bethesda, MD 20892 USA. NIDDK, NIH, Bethesda, MD 20892 USA. RI Gutkind, J. Silvio/A-1053-2009; Hummer, Gerhard/A-2546-2013 OI Hummer, Gerhard/0000-0001-7768-746X NR 0 TC 1 Z9 1 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A270 EP A270 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502143 ER PT J AU Uthus, EO Begaye, A Ross, S Zeng, HW AF Uthus, Eric O. Begaye, Adrienne Ross, Sharon Zeng, Huawei TI The von Hippel-Lindau (VHL) tumor-suppressor gene is downregulated in Caco-2 cells incubated in low-selenium (Se) media SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. Univ Arizona, Tucson, AZ 85705 USA. NIH, NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A717 EP A717 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505577 ER PT J AU Valera, VA Tapia, ELN Teller, L Roberts, DD Linehan, WM Merino, MJ AF Valera, Vladimir Alexander Tapia, Elsa Li-Ning Teller, Linda Roberts, David D. Linehan, W. Marston Merino, Maria J. TI Protein expression profiling in the spectrum of renal tumors SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, Ctr Canc Res, Pathol Lab, Bethesda, MD 20895 USA. NCI, Ctr Canc Res, Urol Oncol Branch, Bethesda, MD 20892 USA. RI Roberts, David/A-9699-2008 OI Roberts, David/0000-0002-2481-2981 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A181 EP A181 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501297 ER PT J AU Vaught, J Schneider, J Lockhart, N Fore, I Moore, H Gillespie, J Scott, E Barker, A Carolyn, C AF Vaught, Jim Schneider, Julie Lockhart, Nicole Fore, Ian Moore, Helen Gillespie, John Scott, Elizabeth Barker, Anna Carolyn, Compton TI NCI's first generation guidelines for biospecimen resources SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Off Biorepositories & Biospecimen Res, Bethesda, MD 20892 USA. NCI, Ctr Bioinformat, Bethesda, MD 20892 USA. Natl Canc Inst, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A64 EP A64 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500306 ER PT J AU Verma, V Shen, DF Zhang, CX Zhou, M Chan, CC Tuo, JS AF Verma, Varun Shen, Defen Zhang, Congxiao Zhou, Min Chan, Chi-Chao Tuo, Jingsheng TI Altered Erp29 and Htra1 in cultured retinal pigment epithelial (RPE) cells of ccl2/cx3cr1 deficient mice - a model of age-related macular degeneration SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC SP Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 NIH, HHMI, Res Scholar Program, Bethesda, MD 20814 USA. NIH, NEI, Immunol Lab, Immunopathol Sect, Bethesda, MD 20892 USA. NIH, NEI, Sect Retinal Dis & Therapeut, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A763 EP A763 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708700134 ER PT J AU Wagner, E Jen, KLC Artiss, J Remaley, AT AF Wagner, Elke Jen, K-L. Catherine Artiss, Joseph Remaley, Alan T. TI Effects of FBCx (R) on lipid lowering in LDLr-KO mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, Lipid Metab Sect, Bethesda, MD 20892 USA. ArtJen Complexus Holdings Corp, Windsor, ON N9A 6V2, Canada. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A341 EP A341 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502486 ER PT J AU Walsh, S Metter, EJ Ferruci, L Roth, SM AF Walsh, Sean Metter, E. Jeffrey Ferruci, Luigi Roth, Stephen M. TI Activin RIIB (ACVR2B) gene associations with muscle mass and strength in humans. SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Maryland, Dept Kinesiol, College Pk, MD 20742 USA. Hartford Hosp, Natl Inst Aging, Clin Res Branch, Baltimore, MD 21225 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1205 EP A1205 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703542 ER PT J AU Wang, PM Martin, WJ AF Wang, Ping Ming Martin, William J., II TI Effect of airway delivered type II alveolar epithelial cells on mice with bleomycin-induced lung injury SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIEHS, Lab Resp Biol, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A555 EP A555 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708504416 ER PT J AU Wang, T Ma, XC Krausz, KW Idle, JR Gonzalez, FJ AF Wang, Ting Ma, Xiaochao Krausz, Kristopher W. Idle, Jeffrey R. Gonzalez, Frank J. TI Role of the pregnane X receptor in the clinical resistance to all-trans retinoic acid SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, Lab Metab, NIH, Bethesda, MD 20892 USA. Charles Univ Prague, Inst Pharmacol, Roztoky, Czech Republic. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1190 EP A1191 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703475 ER PT J AU Weinstein, BM AF Weinstein, Brant M. TI Imaging the developing vasculature in the zebrafish SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NICHD, NIH, Genet Mol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A202 EP A202 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501395 ER PT J AU Weinstein, BM AF Weinstein, Brant M. TI Assembly of endothelial tubes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NICHD, NIH, Genet Mol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A134 EP A134 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501070 ER PT J AU West, SG Kay, CD Gebauer, SK Savastano, DM Diefenbach, CM Kris-Etherton, PM AF West, Sheila G. Kay, Colin D. Gebauer, Sarah K. Savastano, David M. Diefenbach, Chris M. Kris-Etherton, Penny M. TI Pistachios reduce blood pressure and vascular responses to acute stress in healthy adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Penn State Univ, University Pk, PA 16802 USA. NIH, NICHD, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A696 EP A696 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505478 ER PT J AU Whitt, KJ Ling, SM Bos, AJG Muller, DC Roth, SM Ferruccil, L AF Whitt, Karen J. Ling, Shari M. Bos, Angelo J. G. Muller, Denis C. Roth, Stephen M. Ferruccil, Luigi TI The effects of vitamin D receptor polymorphisms on bone mineral density in men and women SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIA, Baltimore, MD 21225 USA. George Mason Univ, Coll Nursing & Hlth Sci, Fairfax, VA 22030 USA. Univ Maryland, Dept Kinesiol, College Pk, MD 20742 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A174 EP A174 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501263 ER PT J AU Wilger, J Radimer, K Burt, V Dwyer, J AF Wilger, Jaime Radimer, Kathy Burt, Vicki Dwyer, Johanna TI Developing a questionnaire,to assess reasons for dietary supplement use. National Health and Nutrition Examination Survey (NHANES) pilot study 2006 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 CDC, Natl Ctr Hlth Stat, Div Hlth Examinat Stat, Hyattsville, MD 20782 USA. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A708 EP A708 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505535 ER PT J AU Xiao, L Zou, TT Liu, L Rao, JN Marasa, BS Gorospe, M Wang, JY AF Xiao, Lan Zou, Tong Tong Liu, Lan Rao, Jaladanki N. Marasa, Bernard S. Gorospe, Myriam Wang, Jian-Ying TI Regulation of ATF-2 mRNA stability by RNA-Binding protein HuR following polyamine depletion in intestinal epithelial cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Maryland, Baltimore, MD 21201 USA. NIA, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A589 EP A589 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708504575 ER PT J AU Xu, XM Carlson, BA Mix, H Zhang, Y Saira, K Glass, RS Berry, MJ Gladyshev, VN Hatfield, DL AF Xu, Xue-Ming Carlson, Bradley A. Mix, Heiko Zhang, Yan Saira, Kazima Glass, Richard S. Berry, Marla J. Gladyshev, Vadim N. Hatfield, Dolph L. TI Biosynthesis of selenocysteine on its tRNA in eukaryotes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NCI, CCR, LCP, Bethesda, MD 20878 USA. Univ Nebraska, Dept Biochem, Lincoln, NE 68583 USA. Univ Arizona, Dept Chem, Tucson, AZ 85721 USA. Univ Hawaii Manoa, Dept Cell & Mol Biol, Honolulu, HI 96822 USA. RI Gladyshev, Vadim/A-9894-2013 NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A113 EP A113 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500542 ER PT J AU Yaniv, K Isogai, S Castranova, D Weinstein, BM AF Yaniv, Karina Isogai, Sumio Castranova, Daniel Weinstein, Brant M. TI Live imaging of lymphatic development in the zebralish embryo SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NICHHD, Mol Genet Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A87 EP A88 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500419 ER PT J AU Yeliseev, AA Zoubak, L Lay, K Gawrisch, K AF Yeliseev, Alexei A. Zoubak, Lioudmila Lay, Kenneth Gawrisch, Klaus TI Bacterial kpression and functional and structural characterization of human peripheral cannabinoid receptor CB2 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAAA, NIH, Rockville, MD 20852 USA. RI Yeliseev, Alexei/B-3143-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A614 EP A614 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505086 ER PT J AU Yeung, ML Yasunaga, JI Bernasser, Y Nelson, D Matsuoka, M Jeang, KT AF Yeung, Man Lung Yasunaga, Jun-ichirou Bernasser, Yannina Nelson, Dusetti Matsuoka, Masao Jeang, Kuan-Teh TI Role of miRNAs (miR-93 and miR-130b) in human T-cell leukemia virus-1 (HTLV-1) transformation of cells through tumor suppressor TP531NP1 downregulation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIAID, Bethesda, MD 20892 USA. Kyoto Univ, Inst Virus Res, Kyoto 606, Japan. INSERM, U624, IFR 137, Inst Cancerol & Immunol, F-13258 Marseille, France. RI Jeang, Kuan-Teh/A-2424-2008 NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1028 EP A1028 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708702285 ER PT J AU Yoo, MH Xu, XM Carlson, BA Gladyshev, VN Hatfield, DL AF Yoo, Min-Hyuk Xu, Xue-Ming Carlson, Bradley A. Gladyshev, Vadim N. Hatfield, Dolph L. TI Examination of anticancer mechanisms in ras-induced cancer cells by targeting thioredoxin reductase 1 knockdown SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, CCR, LCP, Bethesda, MD 20892 USA. Univ Nebraska, Dept Biochem, Lincoln, NE 68583 USA. RI Gladyshev, Vadim/A-9894-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A115 EP A115 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500548 ER PT J AU Youle, RJ AF Youle, Richard J. TI The molecular interrelationships between mitochondrial morphogenesis and apoptosis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, SNB, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A96 EP A96 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708500465 ER PT J AU Young, HA Hodge, D AF Young, Howard A. Hodge, Deborah TI Postfiranscriptional regulation of IFN-gamma gene expressionyy SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, Expt Immunol Lab, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A281 EP A281 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502195 ER PT J AU Yu, MJ Knepper, MA AF Yu, Ming-Jiun Knepper, Mark A. TI LC-MS/MS analysis of rat renal collecting duct membrane proteins affinity-purified with Dolichos biflorus agglutinin SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A477 EP A477 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708504044 ER PT J AU Yue, CM Fields, RL House, S Gainer, H AF Yue, Chunmei Fields, Raymond L. House, Shirley Gainer, Harold TI Oxytocin and Vasopressin heteronuclear RNAs are differentially expressed in the rat supraoptic nucleus SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NIH, NINDS, Neurochem Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1392 EP A1392 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708705304 ER PT J AU Zhang, HM Wess, J Pan, HL AF Zhang, Hong-Mei Wess, Jurgen Pan, Hui-Lin TI Distinct roles of spinal muscarinic receptor subtypes in control of glycinergic input revealed by muscarinic receptor knockout mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Texas, MD Anderson Canc Ctr, Houston, TX 77030 USA. NIDDK, Bioorgan Chem Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A785 EP A785 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708700239 ER PT J AU Zhang, JH Wright, W Bemlohr, DA Cushman, SW Chen, XL AF Zhang, Jinhui Wright, Wendy Bemlohr, David A. Cushman, Samuel W. Chen, Xiaoli TI Alterations of the classic pathway of complement in adipose tissue of obesity and insulin resistance SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Minnesota, St Paul, MN 55108 USA. Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA. NIDDK, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A294 EP A294 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708502258 ER PT J AU Zhang, ZY Bagshaw, D Kris-Etherton, P Lanza, E Hartman, T AF Zhang, Zhiying Bagshaw, Deborah Kris-Etherton, Penny Lanza, Elaine Hartman, Terryl TI Legume inflammation feeding experiment (LIFE) study design and baseline characteristics SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Penn State Univ, Dept Nutr Sci, University Pk, PA 16802 USA. Natl Canc Inst, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A1097 EP A1097 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708703042 ER PT J AU Zhao, CW Andrews, K Schweitzer, A Roseland, J Holden, J Douglass, L Perry, C Dwyer, J Picciano, MF Fisher, K Saldanha, L Yetley, E AF Zhao, Cuiwei Andrews, Karen Schweitzer, Amy Roseland, Janet Holden, Joanne Douglass, Larry Perry, Charles Dwyer, Johanna Picciano, Mary Frances Fisher, Kenneth Saldanha, Leila Yetley, Elizabeth TI Analytical antioxidant content in selected adult multivitamin/mineral (MVM) supplements SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 USDA, Human Nutr Res Ctr, ARS, Nutr Data Lab, Beltsville, MD 20705 USA. Univ Maryland, Biometr Program, College Pk, MD 20742 USA. NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A727 EP A727 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708505624 ER PT J AU Zheng, JG Wang, R Zambraski, E Wu, D Jacobson, K Liang, B AF Zheng, Jingang Wang, Rubio Zambraski, Edward Wu, Dan Jacobson, Kenneth Liang, Bruce TI A novel skeletal muscle cytoprotective action of adenosine A3 receptors SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Univ Connecticut, Sch Med, Farmington, CT 06030 USA. USA, Environm Med Res Inst, Natick, MA 01760 USA. NIH, NIDDK, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 6 BP A800 EP A800 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HF UT WOS:000245708700307 ER PT J AU Zhou, XM Ferraris, JD Burg, MB AF Zhou, Xiaoming Ferraris, Joan D. Burg, Maurice B. TI Expression of MnSOD and Bcl-2 is elevated in the mouse renal medulla. SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA. NHLBI, NIH, LKEM, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A451 EP A451 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708503475 ER PT J AU Zhu, JJ Nguyen, MT Nakamura, E Mackem, S AF Zhu, Jianjian Nguyen, Minh-Thanh Nakamura, Eiichiro Mackem, Susan TI Morphogen or mitogen? Re-evaluating Sonic Hedgehog function in the developing limb SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2007 Annual Meeting CY APR 28-MAY 02, 2007 CL Washington, DC C1 NCI, NIH, Pathol Lab, Bethesda, MD 20892 USA. Univ Occupat & Environm Hlth, Dept Orthoped Surg, Kitakyushu, Fukuoka 8078555, Japan. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2007 VL 21 IS 5 BP A203 EP A204 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157HD UT WOS:000245708501405 ER PT J AU Bonini, MG Siraki, AG Bhattacharjee, S Mason, RP AF Bonini, Marcelo G. Siraki, Arno G. Bhattacharjee, Suchandra Mason, Ronald P. TI Glutathione-induced radical formation on lactoperoxidase does not correlate with the enzyme's peroxidase activity SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article ID OXIDE NITRONE ADDUCT; THYROID PEROXIDASE; HYDROGEN-PEROXIDE; COMPOUND-III; OXIDATION; MYELOPEROXIDASE; SUPEROXIDE; ELECTRON; SYSTEM; THIOLS AB Lactoperoxidase (LPO) is believed to serve as a mediator of host defense against invading pathogens. The protein is more abundant in body fluids such as milk, saliva, and tears. Lactoperoxidase is known to mediate the oxidation of halides and (pseudo)halides in the presence of hydrogen peroxide to reactive intermediates presumably involved in pathogen killing. More recently, LPO has been shown to oxidize a wide diversity of thiol compounds to thiyl free radicals, which ultimately lead to the formation of a protein radical characterized by DMPO-immunospin trapping. In the same study by our group the authors claimed that a consequence of this protein radical formation was the inactivation of LPO (Guo et al., J Biol. Client. 279:13272-13283; 2004). Here we demonstrate that although thiyl radical formation does lead to LPO radical production. the formation of this radical is unrelated to the enzyme's activity. We suggest the source of this misleading interpretation to be the binding of GSH to ELISA plates, which interferes with ABTS and guaiacol oxidation. In addition, DMPO-GSH-nitrone adducts bind to ELISA plates, leading to ambiguities of interpretation since we have demonstrated that DMPO-GSH nitrone does not bind to LPO, and only LPO-protein-DMPO-nitrone adducts can be detected by Western blot. Published by Elsevier Inc. C1 NIEHS, Lab Pharmacol & Chem, NIH, Res Triangle Pk, NC 27709 USA. RP Bonini, MG (reprint author), NIEHS, Lab Pharmacol & Chem, NIH, POB 12233,MD F0-02, Res Triangle Pk, NC 27709 USA. EM bonini@niehs.nih.gov FU Intramural NIH HHS [Z01 ES050139-13] NR 27 TC 11 Z9 11 U1 1 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0891-5849 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PD APR 1 PY 2007 VL 42 IS 7 BP 985 EP 992 DI 10.1016/j.freeradbiomed.2006.12.026 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA 148XX UT WOS:000245111800009 PM 17349926 ER PT J AU Ghany, M Liang, TJ AF Ghany, Marc Liang, T. Jake TI Drug targets and molecular mechanisms of drug resistance in chronic hepatitis B SO GASTROENTEROLOGY LA English DT Review ID HIV-1 REVERSE-TRANSCRIPTASE; ADEFOVIR DIPIVOXIL THERAPY; NUCLEOSIDE NAIVE PATIENTS; IN-VITRO SUSCEPTIBILITY; VIRUS-INFECTION; HBV POLYMERASE; ANGSTROM RESOLUTION; NUCLEOTIDE ANALOGS; CARBOXYPEPTIDASE D; VIRAL REPLICATION AB Chronic hepatitis B continues to be a major cause of end-stage liver disease and hepatocellular carcinoma worldwide. Nucleos(t)ide analogues have proven to be effective in controlling the disease and perhaps decreasing the incidence of hepatocellular carcinoma. However, development of drug resistance is a major limitation to their long-term effectiveness. Understanding the mechanisms of drug resistance are important for designing new agents and devising strategies to manage and prevent the development of antiviral drug resistance. The development of resistance is determined by an interplay of viral, host, and drug characteristics Homology of the HBV polymerase to the human immunodeficiency virus-1 reverse transcriptase has allowed predictions to be made on the effect mutations have on HBV polymerase structure. In vitro functional studies provide complementary information. Several broad principles on the mechanism of resistance have emerged from these studies. First, most of the primary mutations cluster in the vicinity of the incoming nucleotide and act by directly affecting the position or stability of the bound substrate, template, or primer. In contrast, secondary mutations tend to occur away from the nucleotide-binding pocket. Finally, the structural and functional consequences of mutations are quite variable among the different agents. This paper reviews the key mutations and mechanisms associated with resistance to the nucleos(t)ide analogues approved for clinical use and discuss new targets for drug development. C1 NIDDKD, Liver Dis Branch, NIH, Bethesda, MD 20892 USA. RP Ghany, M (reprint author), NIDDKD, Liver Dis Branch, NIH, Bldg 10,Room 9B-16,10 Ctr Dr,MSC 1800, Bethesda, MD 20892 USA. EM marcg@intra.niddk.nih.gov; JakeL@bdg.10.niddk.nih.gov FU Intramural NIH HHS NR 71 TC 97 Z9 110 U1 2 U2 7 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 2007 VL 132 IS 4 BP 1574 EP 1585 DI 10.1053/j.gastro.2007.02.039 PG 12 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 161MZ UT WOS:000246020900041 PM 17408658 ER PT J AU Khalil, IA Kogure, K Futaki, S Hama, S Akita, H Ueno, M Kishida, H Kudoh, M Mishina, Y Kataoka, K Yamada, M Harashima, H AF Khalil, I. A. Kogure, K. Futaki, S. Hama, S. Akita, H. Ueno, M. Kishida, H. Kudoh, M. Mishina, Y. Kataoka, K. Yamada, M. Harashima, H. TI Octaarginine-modified multifunctional envelope-type nanoparticles for gene delivery SO GENE THERAPY LA English DT Article DE non-viral gene delivery system; programmed packaging; octaarginine; multifunctional envelope-type nano device; in vivo topical application; hair growth ID ARGININE-RICH PEPTIDES; INTRACELLULAR TRAFFICKING; HAIR FOLLICLE; FUSOGENIC PEPTIDE; NANO DEVICE; DNA; MACROPINOCYTOSIS; EXPRESSION; LIPOPLEX; SYSTEM AB This study describes a multifunctional envelope-type nano device (MEND) that mimics an envelope-type virus based on a novel packaging strategy. MEND particles contain a DNA core packaged into a lipid envelope modified with an octaarginine peptide. The peptide mediates internalization via macropinocytosis, which avoids lysosomal degradation. MEND-mediated transfection of a luciferase expression plasmid achieved comparable efficiency to adenovirus-mediated transfection, with lower associated cytotoxicity. Furthermore, topical application of MEND particles containing constitutively active bone morphogenetic protein (BMP) type IA receptor (caBmpr1a) gene had a significant impact on hair growth in vivo. These data demonstrate that MEND is a promising non-viral gene delivery system that may provide superior results to existing non-viral gene delivery technologies. C1 Hokkaido Univ, Grad Sch Pharmaceut Sci, Lab Mol Design Pharmaceut, Sapporo, Hokkaido 0600812, Japan. CREST, Shibuya Ku, Tokyo, Japan. Japan Sci & Technol Agcy, JST, Precursory Res Embryon Sci & Technol, Shibuya Ku, Tokyo, Japan. Kyoto Univ, Inst Chem Res, Uji, Kyoto, Japan. Toyama Med & Pharmaceut Univ, Fac Pharmaceut Sci, Sugitani, Toyama 93001, Japan. RIKEN, Brain Sci Inst, Yamada Res Unit, Saitama, Japan. Natl Inst Environm Hlth, Res Triangle Pk, NC USA. Univ Tokyo, Grad Sch Engn, Tokyo, Japan. RP Harashima, H (reprint author), Hokkaido Univ, Grad Sch Pharmaceut Sci, Lab Mol Design Pharmaceut, Kita 12 Nishi 6, Sapporo, Hokkaido 0600812, Japan. EM masahisa@brain.riken.jp; harasima@pharm.hokudai.ac.jp RI Harashima, Hideyoshi/D-8390-2012; Kataoka, Kazunori/K-7108-2012 OI Kataoka, Kazunori/0000-0002-8591-413X FU Intramural NIH HHS; NIEHS NIH HHS [Z01 ES071003-08] NR 37 TC 119 Z9 123 U1 1 U2 17 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0969-7128 J9 GENE THER JI Gene Ther. PD APR PY 2007 VL 14 IS 8 BP 682 EP 689 DI 10.1038/sj.gt.3302910 PG 8 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA 153LO UT WOS:000245436800007 PM 17268535 ER PT J AU Davies, GE Locke, SM Wright, PW Li, H Hanson, RJ Miller, JS Anderson, SK AF Davies, G. E. Locke, S. M. Wright, P. W. Li, H. Hanson, R. J. Miller, J. S. Anderson, S. K. TI Identification of bidirectional promoters in the human KIR genes SO GENES AND IMMUNITY LA English DT Article DE human; NK cells; KIR; transcription ID IG-LIKE RECEPTOR; NATURAL-KILLER NK; T-CELLS; DNA METHYLATION; EXPRESSION; REPERTOIRE; RECOGNITION; MOLECULES; MECHANISM; PATTERNS AB Although the class I MHC receptors expressed by human and mouse natural killer (NK) cells have distinct molecular origins, they are functional analogues that are expressed in a variegated pattern. The murine Ly49 class I receptors contain bidirectional promoters that have been proposed to control the probabilistic expression of these genes. Whether similar elements are present in the human killer Ig-like receptor (KIR) genes is a fundamental question. A detailed analysis of the 2 kb intergenic region separating the KIR2DL4 gene and the adjacent KIR3DL1 gene revealed that additional promoter elements exist in the human KIR genes. Remarkably, the previously characterized KIR3DL1 proximal promoter possesses bidirectional promoter activity that maps to an 88 bp DNA fragment containing CREB, AML, Sp1 and Ets transcription factor binding sites. Individual KIR genes and alleles possess bidirectional promoters with distinct properties. Analysis of KIR+ and KIR- NK cells and NK precursors indicates that reverse transcripts from the bidirectional promoter are found in cells that lack KIR protein expression, but are not present in mature KIR- expressing NK cells, suggesting that reverse transcription from the proximal promoter blocks gene activation in immature NK and precursor cells. C1 NCI, Basic Res Program, SAIC Frederick Inc, Ft Detrick, MD 21702 USA. NCI, Lab Expt Immunol, Ctr Canc Res, Ft Detrick, MD 21702 USA. Univ Minnesota, Div Hematol Oncol & Transplantat, Minneapolis, MN 55455 USA. RP Anderson, SK (reprint author), NCI, Lab Expt Immunol, Ctr Canc Res, Bldg 560,Room 31-93, Ft Detrick, MD 21702 USA. EM andersonst@mail.nih.gov RI Anderson, Stephen/B-1727-2012; OI Anderson, Stephen/0000-0002-7856-4266; Miller, Jeffrey S/0000-0002-0339-4944 FU Intramural NIH HHS; NCI NIH HHS [N01-CO-12400]; NHLBI NIH HHS [R01 HL55417] NR 33 TC 45 Z9 45 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1466-4879 J9 GENES IMMUN JI Genes Immun. PD APR PY 2007 VL 8 IS 3 BP 245 EP 253 DI 10.1038/sj.gene.6364381 PG 9 WC Genetics & Heredity; Immunology SC Genetics & Heredity; Immunology GA 160VM UT WOS:000245970400008 PM 17315044 ER PT J AU Rempel, SA Hawley, RC Gutierrez, JA Mouzon, E Bobbitt, KR Lemke, N Schultz, CR Schultz, LR Golembieski, W Koblinski, J VanOsdol, S Miller, CG AF Rempel, S. A. Hawley, R. C. Gutierrez, J. A. Mouzon, E. Bobbitt, K. R. Lemke, N. Schultz, C. R. Schultz, L. R. Golembieski, W. Koblinski, J. VanOsdol, S. Miller, C. G. TI Splenic and immune alterations of the Sparc-null mouse accompany a lack of immune response SO GENES AND IMMUNITY LA English DT Article DE SPARC; Sparc-null mouse; immune cells; spleen; marginal zone ID ZONE B-CELLS; HUMAN-MELANOMA CELLS; EXTRACELLULAR-MATRIX; ENHANCED GROWTH; MICE DEFICIENT; CYSTEINE SPARC; IN-VITRO; PROTEIN; RICH; EXPRESSION AB Sparc-null mice have been used as models to assess tumor-host immune cell interactions. However, it is not known if they have a competent immune system. In this study, the immune systems of Sparc wild-type and null mice were compared. Mice were assessed for differences in total body weight, spleen weight and spleen-to-body weight ratios. Spleens were compared with respect to morphology, and Sparc, Ki-67, MOMA-1 and IgM expression. Immune cells in blood, bone marrow and spleen were assessed by blood smears, automated blood panel, and flow cytometry. Additionally, the ability of Sparc-null mice to respond to immune challenge was evaluated using a footpad model. The morphological and immunohistochemical results indicated that Sparc-null spleens had more white pulp, hyperproliferative B cells in the germinal centers, and decreased marginal zones. Sparc-null spleens lacked normal Sparc expression in red and white pulp, marginal zones, endothelial and sinusoidal cells. By flow analysis, B cells were decreased and T cells were increased in the bone marrow. Finally, Sparc-null mice were unable to mount an immune response following footpad lipopolysaccharide challenge. These data confirm that Sparc-null mice have an impaired immune system. C1 Henry Ford Hosp, Dept Pathol, Detroit, MI 48202 USA. Henry Ford Hosp, Dept Neurosurg, Hermelin Brain Tumor Ctr, Barbara Jane Levy Lab Mol Neurooncol, Detroit, MI 48202 USA. Henry Ford Hosp, Dept Neurosurg, Hermelin Brain Tumor Ctr, Gayle Halperin Kahn Lab Viral Oncotherapeut, Detroit, MI 48202 USA. Henry Ford Hosp, Dept Biostat & Res Epidemiol, Detroit, MI 48202 USA. NIDCR, NIH, Bethesda, MD USA. RP Rempel, SA (reprint author), Henry Ford Hosp, Dept Neurosurg, Hermelin Brain Tumor Ctr, Barbara Jane Levy Lab Mol Neurooncol, 2799 W Grand Blvd, Detroit, MI 48202 USA. EM nssan@neuro.hfh.edu FU NCI NIH HHS [CA086997, R01 CA086997] NR 38 TC 22 Z9 24 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1466-4879 J9 GENES IMMUN JI Genes Immun. PD APR PY 2007 VL 8 IS 3 BP 262 EP 274 DI 10.1038/sj.gene.6364388 PG 13 WC Genetics & Heredity; Immunology SC Genetics & Heredity; Immunology GA 160VM UT WOS:000245970400010 PM 17344888 ER PT J AU Kreslova, J Machon, O Ruzickova, J Lachova, J Wawrousek, EF Kemler, R Krauss, S Piatigorsky, J Kozmik, Z AF Kreslova, Jana Machon, Ondrej Ruzickova, Jana Lachova, Jitka Wawrousek, Eric F. Kemler, Rolf Krauss, Stefan Piatigorsky, Joram Kozmik, Zbynek TI Abnormal lens morphogenesis and ectopic lens formation in the absence of beta-catenin function SO GENESIS LA English DT Article DE Wnt signaling; beta-catenin; eye; lens; retina ID CELL-DIFFERENTIATION; MOUSE DEVELOPMENT; EXPRESSION; GENE; WNT; PAX6; MICE; EYE; ROLES; CHX10 AB beta-Catenin plays a key role in cadherin-mediated cell adhesion as well as in canonical Wnt signaling. To study the role of beta-catenin during eye development, we used conditional Cre/loxP system in mouse to inactivate beta-catenin in developing lens and retina. Inactivation of beta-catenin does not suppress lens fate, but instead results in abnormal morphogenesis of the lens. Using BAT-gal reporter mice, we show that beta-catenin-mediated Wnt signaling is notably absent from lens and neuroretina throughout eye development. The observed defect is therefore likely due to the cytoskeletal role of beta-catenin, and is accompanied by impaired epithelial cell adhesion. In contrast, inactivation of beta-catenin in the nasal ectoderm, an area with active Wnt signaling, results in formation of crystallin-positive ectopic lentoid bodies. These data suggest that, outside of the normal lens, beta-catenin functions as a coactivator of canonical Wnt signaling to suppress lens fate. C1 Acad Sci Czech Republ, Inst Mol Genet, Prague 14220 4, Czech Republic. NEI, NIH, Bethesda, MD 20892 USA. Max Planck Inst Immunobiol, D-7800 Freiburg, Germany. Univ Oslo, Rikshosp, Inst Microbiol, N-0027 Oslo, Norway. RP Kozmik, Z (reprint author), Acad Sci Czech Republ, Inst Mol Genet, Videnska 1083, Prague 14220 4, Czech Republic. EM kozmik@img.cas.cz RI Wawrousek, Eric/A-4547-2008; Machon, Ondrej/G-3587-2014; Kozmik, Zbynek/G-3581-2014; Kozmik, Zbynek/I-8807-2014 NR 37 TC 38 Z9 38 U1 0 U2 2 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 1526-954X J9 GENESIS JI Genesis PD APR PY 2007 VL 45 IS 4 BP 157 EP 168 DI 10.1002/dvg.20277 PG 12 WC Developmental Biology; Genetics & Heredity SC Developmental Biology; Genetics & Heredity GA 161MQ UT WOS:000246019800001 PM 17410548 ER PT J AU Chen, JB Yu, K Hsing, A Therneau, TM AF Chen, Jinbo Yu, Kai Hsing, Ann Therneau, Terry M. TI A partially linear tree-based regression model for assessing complex joint gene-gene and gene-environment effects SO GENETIC EPIDEMIOLOGY LA English DT Article DE gene-gene interaction; gene-environment interaction; generalized linear model; tree model; partially linear ID ASSOCIATION; EPISTASIS; POWER; HAPLOTYPES; SNPS; MDR AB The success of genetic dissection of complex diseases may greatly benefit from judicious exploration of joint gene effects, which, in turn, critically depends on the power of statistical tools. Standard regression models are convenient for assessing main effects and low-order gene-gene interactions but not for exploring complex higher-order interactions. Tree-based methodology is an attractive alternative for disentangling possible interactions, but it has difficulty in modeling additive main effects. This work proposes a new class of semiparametric regression models, termed partially linear tree-based regression (PLTR) models, which exhibit the advantages of both generalized linear regression and tree models. A PLTR model quantifies joint effects of genes and other risk factors by a combination of linear main effects and a non-parametric tree-structure. We propose an iterative algorithm to fit the PLTR model, and a unified resampling approach for identifying and testing the significance of the optimal "pruned" tree nested within the tree resultant from the fitting algorithm. Simulation studies showed that the resampling procedure maintained the correct type I error rate. We applied the PLTR model to assess the association between biliary stone risk and 53 single nucleotide polymorphisms. (SNPs) in the inflammation pathway in a population-based case-control study. The analysis yielded an interesting parsimonious summary of the joint effect of all SNPs. The proposed model is also useful for exploring gene-environment interactions and has broad implications for applying the tree methodology to genetic epidemiology research. C1 Univ Penn, Sch Med, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA. NCI, Biostat Branch, Div Canc Epidemiol & Genet, Rockville, MD USA. NCI, Hormonal & Reprod Epidemiol Branch, Div Canc Epidemiol & Genet, Rockville, MD USA. Dept Hlth Sci Res, Div Biostat, Rochester, MN USA. RP Chen, JB (reprint author), Univ Penn, Sch Med, Dept Biostat & Epidemiol, 423 Guardian Dr, Philadelphia, PA 19104 USA. EM jchen@cceb.med.upenn.edu NR 29 TC 13 Z9 13 U1 0 U2 4 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0741-0395 J9 GENET EPIDEMIOL JI Genet. Epidemiol. PD APR PY 2007 VL 31 IS 3 BP 238 EP 251 DI 10.1002/gepi.20205 PG 14 WC Genetics & Heredity; Mathematical & Computational Biology SC Genetics & Heredity; Mathematical & Computational Biology GA 149DW UT WOS:000245128200006 PM 17266115 ER PT J AU King, V Goodfellow, PN Wilkerson, AJP Johnson, WE O'Brien, SJ Pecon-Slattery, J AF King, V. Goodfellow, P. N. Wilkerson, A. J. Pearks Johnson, W. E. O'Brien, S. J. Pecon-Slattery, J. TI Evolution of the male-determining gene SRY within the cat family Felidae SO GENETICS LA English DT Article ID SEX-DETERMINING REGION; TESTIS-DETERMINING GENE; MAMMALIAN Y-CHROMOSOME; GONADAL-DYSGENESIS; POSITIVE SELECTION; DETERMINING LOCUS; REVERSED FEMALES; SEQUENCES; RADIATION; PRIMATES AB In most placental mammals, SRY is a single-copy gene located on the Y chromosome and is the trigger for male sex determination during embryonic development. Here, we present comparative genomic analyses of SRY (705 bp) along with the adjacent noncoding 5 ' flank (997 bp) and 3 ' flank (948 bp) in 36 species of the cat family Felidae. Phylogenetic analyses indicate that the noncoding genomic flanks and SHY closely track species divergence. However, several inconsistencies are observed in SRY Overall, the gene exhibits purifying selection to maintain function (omega = 0.815) yet SRY is under positive selection in two of the eight felid lineages. SRY has low numbers of nucleotide substitutions, yet most encode amino acid changes between species, and four different species have significantly altered SRY due to insertion/ deletions. Moreover, fixation of nonsynonymous substitutions between sister taxa is not consistent and may occur rapidly, as in the case of domestic cat, or not at all over long periods of time, as observed within the Panthera lineage. The former resembles positive selection during speciation, and the latter purifying selection to maintain function. Thus, SRY evolution in cats likely reflects the different phylogeographic histories, selection pressures, and patterns of speciation in modern felids. C1 NCI, Lab Genom Divers, Frederick, MD 21702 USA. Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England. RP Pecon-Slattery, J (reprint author), NCI, Lab Genom Divers, Bldg 560,Room 11-10, Frederick, MD 21702 USA. EM pngoodfellow@yahoo.com; slattery@mail.ncifcrf.gov RI Johnson, Warren/D-4149-2016 OI Johnson, Warren/0000-0002-5954-186X FU Intramural NIH HHS; NCI NIH HHS [N01-CO-12400, N01CO12400]; Wellcome Trust NR 62 TC 14 Z9 21 U1 4 U2 8 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD APR PY 2007 VL 175 IS 4 BP 1855 EP 1867 DI 10.1534/genetics.106.066779 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA 167JU UT WOS:000246448800027 PM 17277366 ER PT J AU Nag, N Peterson, K Wyatt, K Hess, S Ray, S Favor, J Bogani, D Lyon, M Wistow, G AF Nag, Nabanita Peterson, Katherine Wyatt, Keith Hess, Sonja Ray, Sugata Favor, Jack Bogani, Debora Lyon, Mary Wistow, Graeme TI Endogenous retroviral insertion in Cryge in the mouse No3 cataract mutant SO GENOMICS LA English DT Article DE cataract; crystallin; endogenous retrovirus ID GAMMA-D-CRYSTALLIN; SEQUENCE TAG ANALYSIS; X-RAY-ANALYSIS; NEIBANK PROJECT; DOMINANT CATARACT; EYE LENS; GENE; MUTATION; ELEMENTS; TRANSCRIPTS AB No3 (nuclear opacity 3) is a novel congenital nuclear cataract in mice. Microsatellite mapping placed the No3 locus on chromosome 1 between DlMit480 (32cM) and DlMit7 (41cM), a region containing seven crystallin genes; Cryba2 and the Cryga-Crygf cluster. Although polymorphic variants were observed, no candidate mutations were found for six of the genes. However, DNA walking identified a murine endogenous retrovirus (IAPLTR1: ERVK) insertion in exon 3 of Cryge, disrupting the coding sequence for gamma E-crystallin. Recombinant protein for the mutant gamma E was completely insoluble. The NO cataract is mild compared with the effects of similar mutations of gamma E. Quantitative RT-PCR showed that gamma E/F mRNA levels are reduced in No3, suggesting that the relatively mild phenotype results from suppression of gamma E levels due to ERVK insertion. However, the severity of cataract is also strain dependent suggesting that genetic background modifiers also play a role in the development of opacity. (c) 2006 Elsevier Inc. All rights reserved. C1 NEI, Sect Mol Struct & Funct Genom, NIH, Bethesda, MD 20892 USA. NIDDK, Proteom & Mass Spectrometry Facil, NIH, Bethesda, MD 20892 USA. GSF Natl Res Ctr Environm & Hlth, Inst Human Genet, D-85764 Neuherberg, Germany. MRC, Didcot OX11 0RD, Oxon, England. RP Wistow, G (reprint author), NEI, Sect Mol Struct & Funct Genom, NIH, Bethesda, MD 20892 USA. EM graeme@helix.nih.gov RI Hess, Sonja/K-4842-2013 OI Hess, Sonja/0000-0002-5904-9816 FU Medical Research Council [MC_U142684172, MC_U142684175] NR 35 TC 4 Z9 4 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0888-7543 J9 GENOMICS JI Genomics PD APR PY 2007 VL 89 IS 4 BP 512 EP 520 DI 10.1016/j.ygeno.2006.12.003 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 155EF UT WOS:000245560000008 PM 17223009 ER PT J AU Qin, LY Wu, XF Block, ML Liu, YX Breese, GR Hong, JS Knapp, DJ Crews, FT AF Qin, Liya Wu, Xuefei Block, Michelle L. Liu, Yuxin Breese, George R. Hong, Jau-Shyong Knapp, Darin J. Crews, Fulton T. TI Systemic LPS causes chronic neuroinflammation and progressive neurodegeneration SO GLIA LA English DT Article DE TNF alpha; LPS; substantia nigra; microglia; neurodegeneration; neuroinflammation ID TUMOR-NECROSIS-FACTOR; TNF-ALPHA TRANSPORT; BLOOD-BRAIN-BARRIER; LIPOPOLYSACCHARIDE-INDUCED NEUROTOXICITY; INFLAMMATION-MEDIATED NEURODEGENERATION; CORTICOTROPIN-RELEASING FACTOR; AMYOTROPHIC-LATERAL-SCLEROSIS; BACTERIAL-ENDOTOXIN EXPOSURE; INNATE IMMUNE-SYSTEM; DOPAMINE NEURON LOSS AB Inflammation is implicated in the progressive nature of neurodegenerative diseases, such as Parkinson's disease, but the mechanisirts are poorly understood. A single systemic lipopolysaccharide (LPS, 5 mg/kg, i.p.) or tumor necrosis factor alpha (TNF alpha, 0.25 mg/kg, i.p.) injection was administered in adult wild-type mice and in mice lacking TNF alpha receptors (TNF R1/R2(-/-)) to discern the mechanisms of inflammation transfer from the periphery to the brain and the neurodegenerative consequences. Systemic LPS administration resulted in rapid brain TNFa increase that remained elevated for 10 months, while peripheral TNF alpha (serum and liver) had subsided by 9 h (serum) and 1 week (liver). Systemic TNF alpha and LPS administration activated microglia and increased expression of brain pro-inflammatory factors (i.e., TNF alpha, MCP-1, IL-1 beta and NF-kappa B p65) in wild-type mice, but not in TNF R1/R2(-/-) mice. Further, LPS reduced the number of tyrosine hydroxylase-immunoreactive neurons in the substantia nigra (SN) by 23% at 7-months post-treatment, which progressed to 47% at 10 months. Together, these data demonstrate that through TNF alpha, peripheral inflammation in adult animals can: (1) activate brain microglia to produce chronically elevate roinflammatory factors; (2) induce delayed and progressive loss of DA neurons in the SN. These findings provide valuable insight into the potential pathogenesis and self-propelling nature of Parkinson's disease. (c) 2007 Wiley-Liss, Inc. C1 Univ N Carolina, Bowles Ctr Alcohol Studies, Sch Med, Chapel Hill, NC 27599 USA. Dalian Med Univ, Dept Physiol, Dalian, Peoples R China. Natl Inst Environm Hlth Sci, Neuropharmacol Sect, Res Triangle Pk, NC USA. Univ N Carolina, Sch Med, Dept Psychiat, Chapel Hill, NC 27599 USA. Univ N Carolina, Sch Med, Dept Pharmacol, Chapel Hill, NC 27599 USA. RP Crews, FT (reprint author), Univ N Carolina, Bowles Ctr Alcohol Studies, Sch Med, 1021 Thurston Bowles Bldg,CB 7178, Chapel Hill, NC 27599 USA. EM ftcrews@md.unc.edu FU NIAAA NIH HHS [P60 AA011605, P60 AA011605-070005] NR 48 TC 673 Z9 695 U1 20 U2 111 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0894-1491 EI 1098-1136 J9 GLIA JI Glia PD APR 1 PY 2007 VL 55 IS 5 BP 453 EP 462 DI 10.1002/glia.20467 PG 10 WC Neurosciences SC Neurosciences & Neurology GA 134SQ UT WOS:000244104700001 PM 17203472 ER PT J AU Shi, ZG Henwood, MJ Bannermam, P Batista, D Horvath, A Guttenberg, M Stratakis, CA Grimberg, A AF Shi, Zonggao Henwood, Maria J. Bannermam, Peter Batista, Dalia Horvath, Anelia Guttenberg, Marta Stratakis, Constantine A. Grimberg, Adda TI Primary pigmented nodular adrenocortical disease reveals insulin-like growth factor binding protein-2 regulation by protein kinase A SO GROWTH HORMONE & IGF RESEARCH LA English DT Article DE insulin-like growth factor binding protein (IGFBP)-2; cAMP dependent protein kinase (PKA); PRKAR1A gene; adrenocortical cells; NCI-H295R cells; primary pigmented nodular adrenocortical disease (PPNAD) ID CAMP-RESPONSE ELEMENT; CARNEY COMPLEX; TUMOR-CELLS; EPITHELIAL-CELLS; CUSHING-SYNDROME; CANCER CELLS; KAPPA-B; IGF-II; GENE; PROLIFERATION AB Objective: Primary pigmented nodular adrenocortical disease (PPNAD) can occur as an isolated trait or part of Carney complex, a familial lentiginosis-multiple endocrine neoplasia syndrome frequently caused by mutations in PRKAR1A, which encodes the 1 alpha regulatory subunit of protein kinase A (PKA). Because alterations in the insulin-like growth factor (IGF) axis, particularly IGF-II and IGF binding protein (IGFBP)-2 overexpression, have been implicated in sporadic adrenocortical tumors, we sought to examine the IGF axis in PPNAD. Design: RNA samples and paraffin-embedded sections were procured from adrenalectomy specimens of patients with PPNAD. Changes in expression of IGF axis components were evaluated by real-time quantitative RT-PCR and immunohistochemistry. NCI-H295R cells were used to study PKA and IGF axis signaling in adrenocortical cells in vitro. Results: IGFBP-2 mRNA level distinguished between the two genetic subtypes of this disease; increased IGFBP-2 expression in PRKAR1A mutation-positive PPNAD tissues was also confirmed by immunohistochemistry. Moreover, PKA inhibitors increased IGFBP-2 expression in NCI-H295R adrenocortical cells, and anti-IGFBP-2 antibody reduced their proliferation. Conclusions: IGFBP-2 expression is increased in PPNAD caused by PRKAR1A mutations, and in adrenocortical cancer cells. This is the first evidence for PKA-dependent regulation of IGFBP-2 expression in adrenocortical cells. C1 Univ Penn, Childrens Hosp Philadelphia, Sch Med, Div Pediat Endocrinol, Philadelphia, PA 19104 USA. Childrens Hosp Philadelphia, Abramson Res Ctr, Div Neurol Res, Philadelphia, PA 19104 USA. NICHHD, Sect Endocrinol & Genet, Bethesda, MD 20892 USA. Childrens Hosp Philadelphia, Dept Pathol, Philadelphia, PA 19104 USA. RP Grimberg, A (reprint author), Univ Penn, Childrens Hosp Philadelphia, Sch Med, Div Pediat Endocrinol, Abrahamson Res Ctr Room 802,3615 Civic Ctr Blvd, Philadelphia, PA 19104 USA. EM grimberg@email.chop.edu FU NICHD NIH HHS [Z01 HD000642, Z01 HD000642-04]; NIDDK NIH HHS [K08 DK064352, T32 DK63688, 5 K08 DK64352, T32 DK063688] NR 46 TC 3 Z9 3 U1 0 U2 0 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND SN 1096-6374 J9 GROWTH HORM IGF RES JI Growth Horm. IGF Res. PD APR PY 2007 VL 17 IS 2 BP 113 EP 121 DI 10.1016/j.ghir.2006.12.004 PG 9 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA 167JH UT WOS:000246447500004 PM 17280861 ER PT J AU Elder, JP Lytle, L Sallis, JF Young, DR Steckler, A Simons-Morton, D Stone, E Jobe, JB Stevens, J Lohman, T Webber, L Pate, R Saksvig, BI Ribisl, K AF Elder, John P. Lytle, Leslie Sallis, James F. Young, Deborah Rohm Steckler, Allan Simons-Morton, Denise Stone, Elaine Jobe, Jared B. Stevens, June Lohman, Tim Webber, Larry Pate, Russell Saksvig, Brit I. Ribisl, Kurt TI A description of the social-ecological framework used in the trial of activity for adolescent girls (TAAG) SO HEALTH EDUCATION RESEARCH LA English DT Article ID HEALTH PROMOTION PROGRAMS; PHYSICAL-ACTIVITY; CARDIOVASCULAR HEALTH; EDUCATION; BEHAVIORS; CHILDREN; INTERVENTIONS; DETERMINANTS; ENVIRONMENTS; SCHOOLS AB Social-ecological (SE) models are becoming more widely used in health behavior research. Applying SE models to the design of interventions is challenging because models must be tailor-made for each behavior and population, other theories need to be integrated into multi-level frameworks, and empirical research to guide model development is limited. The purpose of the present paper is to describe a SE framework that guided the intervention and measurement plans for a specific study. The trial of activity for adolescent girls (TAAG) is a multi-center study of interventions to reduce the decline of physical activity in adolescent girls. The TAAG framework incorporates operant learning theory, social cognitive theory, organizational change theory and the diffusion of innovation model in a multi-level model. The explicit and practical model developed for TAAG has already benefited the study and may have elements that can generalize to other health promotion studies. C1 San Diego State Univ, Grad Sch Publ Hlth, Div Hlth Promot, San Diego, CA 92123 USA. Univ Minnesota, Sch Publ Hlth, Div Epidemiol, Minneapolis, MN 55454 USA. San Diego State Univ, Dept Psychol, San Diego, CA 92103 USA. Univ Maryland, Dept Kinesiol, College Pk, MD 20742 USA. Univ N Carolina, Dept Hlth Behav & Hlth Educ, Chapel Hill, NC 27599 USA. NHLBI, Clin Applicat & Prevetn Program, Bethesda, MD 20892 USA. Univ New Mexico, Dept Phys Performance & Dev, Albuquerque, NM 87131 USA. Univ N Carolina, Dept Nutr, Chapel Hill, NC 27599 USA. Univ N Carolina, Dept Epidemiol, Chapel Hill, NC 27599 USA. Univ Arizona, Tucson, AZ 85721 USA. Tulane Univ, Sch Publ Hlth & Trop Med, Dept Biostat, New Orleans, LA 70112 USA. Univ S Carolina, Arnold Sch Publ, Dept Exercise Sci, Columbia, SC 29208 USA. RP Elder, JP (reprint author), San Diego State Univ, Grad Sch Publ Hlth, Div Hlth Promot, San Diego, CA 92123 USA. EM jelder@projects.sdsu.edu RI Ribisl, Kurt/C-4867-2015 OI Ribisl, Kurt/0000-0003-3318-8524 FU NHLBI NIH HHS [U01HL066845, U01 HL066845, U01 HL066852, U01 HL066853, U01 HL066855, U01 HL066856, U01 HL066856-01, U01 HL066857, U01 HL066858, U01HL066852, U01HL066853, U01HL066855, U01HL066856, U01HL066857, U01HL066858] NR 48 TC 88 Z9 89 U1 2 U2 20 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0268-1153 J9 HEALTH EDUC RES JI Health Educ. Res. PD APR PY 2007 VL 22 IS 2 BP 155 EP 165 DI 10.1093/her/cyl059 PG 11 WC Education & Educational Research; Public, Environmental & Occupational Health SC Education & Educational Research; Public, Environmental & Occupational Health GA 146VH UT WOS:000244962300001 PM 16855014 ER PT J AU Hoofnagle, JH Doo, E Liang, TJ Fleischer, R Lok, ASF AF Hoofnagle, Jay H. Doo, Edward Liang, T. Jake Fleischer, Russell Lok, Anna S. F. TI Management of hepatitis B: Summary of a clinical research workshop SO HEPATOLOGY LA English DT Review ID TENOFOVIR DISOPROXIL FUMARATE; E-ANTIGEN SEROCONVERSION; TERM-FOLLOW-UP; HUMAN-IMMUNODEFICIENCY-VIRUS; LAMIVUDINE-RESISTANT PATIENTS; BONE-MARROW-TRANSPLANTATION; ADEFOVIR DIPIVOXIL THERAPY; HBEAG(+) CHRONIC HEPATITIS; ALPHA-INTERFERON TREATMENT; HIV-INFECTED PATIENTS AB Chronic hepatitis B is caused by persistent infection with the hepatitis B virus (HBV), a unique DNA vim that replicates through an RNA intermediate produced from a stable covalently dosed circular DNA molecule. Viral persistence appears to be due to inadequate innate and adaptive immune responses. Chronic infection has a variable course after several decades resulting in cirrhosis in up to one-third of patients and liver cancer in a proportion of those with cirrhosis. Sensitive assays for HBV DNA levels in serum have been developed that provide important insights into pathogenesis and natural history. Therapy of hepatitis B is evolving. Peginterferon induces long-term remissions in disease in one-third of patients with typical hepatitis B e antigen (HBeAg) positive chronic hepatitis B, but a lesser proportion of those without HBeAg. Several oral nucleoside analogues with activity against HBV have been shown to be effective in suppressing viral levels and improving biochemical and histological features of disease in a high proportion of patients with and without HBeAg, at least in the short term. What is uncertain is which agent or combination of agents is most effective, how long therapy should last, and which criteria should be used to start, continue, switch or stop therapy. Long-term therapy with nucleoside analogues may be the most appropriate approach to treatment, but the expense and lack of data on long-term safety and efficacy make recommendations difficult. Clearly, many basic and clinical research challenges remain in defining optimal means of management of chronic hepatitis B. C1 NIDDK, Liver Dis Res Branch, NIH, Bethesda, MD 20892 USA. NIDDK, Liver Dis Branch, NIH, Bethesda, MD 20892 USA. US FDA, Div Antiviral Prod, Silver Spring, MD USA. Univ Michigan, Med Ctr, Div Gastroenterol, Ann Arbor, MI 48109 USA. RP Hoofnagle, JH (reprint author), NIDDK, Liver Dis Res Branch, NIH, Bldg 31,Room 9A27,31 Ctr Dr, Bethesda, MD 20892 USA. EM HoofnagleJ@extra.niddk.nih.gov RI Lok, Anna /B-8292-2009; OI Yang, Shuman/0000-0002-9638-0890 FU Intramural NIH HHS NR 187 TC 352 Z9 413 U1 5 U2 33 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD APR PY 2007 VL 45 IS 4 BP 1056 EP 1075 DI 10.1002/hep.21627 PG 20 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 154MS UT WOS:000245512300026 PM 17393513 ER PT J AU Zezula, J Jacobson, AE Rice, KC AF Zezula, Josef Jacobson, Arthur E. Rice, Kenner C. TI A novel divergent synthesis of ortho-hydroxy-E and -F oxide-bridged 5-phenylmorphans SO HETEROCYCLES LA English DT Article AB 5-(2-Bromo-3-methoxyphenyl)-2-methyl-2-azabicyclo[3.3.1]nonan-9-one was prepared in six steps from a known phenylacetonitrile. Stereoselective reduction of the ketone furnished the corresponding beta- or alpha-alcohols and their deprotonation, intramolecular cyclization, and demethylation gave ortho-hydroxy-e and -f oxide-bridged 5-phenylmorphans, respectively. This new synthetic route has the desired oxygenation pattern in place, eliminating the problematic diazonium reactions used in former syntheses. C1 NIDA, NIH, Drug Design & Synth Sect, Chem Biol Res Branch,Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Rice, KC (reprint author), NIDA, NIH, Drug Design & Synth Sect, Chem Biol Res Branch,Dept Hlth & Human Serv, Bldg 8,Room B 1-23, Bethesda, MD 20892 USA. EM kr21f@nih.gov NR 8 TC 7 Z9 7 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0385-5414 J9 HETEROCYCLES JI Heterocycles PD APR 1 PY 2007 VL 71 IS 4 BP 881 EP 889 PG 9 WC Chemistry, Organic SC Chemistry GA 165MZ UT WOS:000246310900009 ER PT J AU Boyes, WK Moser, VC Geller, AM Benignus, VA Bushnell, PJ Kamel, F AF Boyes, William K. Moser, Virginia C. Geller, Andrew M. Benignus, Vernon A. Bushnell, Philip J. Kamel, Freya TI Integrating epidemiology and toxicology in neurotoxicity risk assessment SO HUMAN & EXPERIMENTAL TOXICOLOGY LA English DT Article; Proceedings Paper CT 10th Meeting of the International-Neurotoxicology-Association CY JUN 06, 2005-JUL 01, 2006 CL Haikko, FINLAND SP Int Neurotoxicol Assoc DE animal studies; epidemiology; neurotoxicity risk assessment; toxicology ID PESTICIDE APPLICATORS; AGRICULTURAL HEALTH; RETINAL DEGENERATION; VISUAL FUNCTION; ANIMAL-MODELS; EXPOSURE; RAT; TRICHLOROETHYLENE; DYSFUNCTION; BATTERIES AB Neurotoxicity risk assessments depend on the best available scientific information, including data from animal toxicity studies, human experimental studies and human epidemiology studies. There are several factors to consider when evaluating the comparability of data from studies. Regarding the epidemiology literature, issues include choice of study design, use of appropriate controls, methods of exposure assessment, subjective or objective evaluation of neurological status, and assessment and statistical control of potential confounding factors, including co-exposure to other agents. Animal experiments must be evaluated regarding factors such as dose level and duration, procedures used to assess neurological or behavioural status, and appropriateness of inference from the animal model to human neurotoxicity. Major factors that may explain apparent differences between animal and human studies include: animal neurological status may be evaluated with different procedures than those used in humans; animal studies may involve shorter exposure durations and higher dose levels; and most animal studies evaluate a single substance whereas humans typically are exposed to multiple agents. The comparability of measured outcomes in animals and humans may be improved by considering functional domains rather than individual test measures. The application of predictive models, weight of evidence considerations and meta-analysis can help evaluate the consistency of outcomes across studies. An appropriate blend of scientific information from toxicology and epidemiology studies is necessary to evaluate potential human risks of exposure to neurotoxic substances. C1 US EPA, Neurotoxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA. NIEHS, Res Triangle Pk, NC 27709 USA. RP Boyes, WK (reprint author), US EPA, Neurotoxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, B105-05, Res Triangle Pk, NC 27711 USA. EM boyes.william@epa.gov OI Kamel, Freya/0000-0001-5052-6615 NR 34 TC 4 Z9 4 U1 0 U2 0 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0960-3271 J9 HUM EXP TOXICOL JI Hum. Exp. Toxicol. PD APR PY 2007 VL 26 IS 4 BP 283 EP 293 DI 10.1177/0960327106070481 PG 11 WC Toxicology SC Toxicology GA 166XG UT WOS:000246413500003 PM 17615109 ER PT J AU Aker, M Tubb, J Groth, AC Bukovsky, AA Bell, AC Felsenfeld, G Kiem, HP Stamatoyannopoulos, G Emery, DW AF Aker, Mari Tubb, Julie Groth, Amy C. Bukovsky, Anatoly A. Bell, Adam C. Felsenfeld, Gary Kiem, Hans-Peter Stamatoyannopoulos, George Emery, David W. TI Extended core sequences from the cHS4 insulator are necessary for protecting retroviral vectors from silencing position effects SO HUMAN GENE THERAPY LA English DT Article ID BETA-GLOBIN INSULATOR; GENE-TRANSFER; CHROMATIN INSULATOR; LENTIVIRAL VECTORS; ENHANCER BLOCKING; GAMMA-GLOBIN; THALASSEMIA; CELLS; CTCF; INTEGRATION AB The prototypic chromatin insulator cHS4 has proven effective at reducing repressive chromosomal position effects on retroviral vector expression. We report here studies designed to identify the minimal chicken hypersensitive site-4 (cHS4) sequences necessary for this activity. Using a gammaretroviral reporter vector and expression analysis in cell lines and primary mouse hematopoietic progenitor colonies, we found that a 250bp core fragment reported to contain most of the cHS4 insulating activity failed to prevent silencing when used alone, although some barrier activity was observed when this fragment was combined with a 790-bp, but not 596-bp, spacer. Similar studies showed that four copies of a 90-bp fragment containing the cHS4 enhancer-blocking activity actually repressed vector green fluorescent protein (GFP) expression. In contrast, a 400-bp fragment containing the 250-bp core plus 3' flanking sequences protected vector expression to the same degree as the full-length 1.2-kb fragment. The 400-bp fragment activity was confirmed in a lentiviral vector expressing human beta-globin in murine erythroid leukemia (MEL) cells. Taken together, these studies indicate that the insulating activity of the 250-bp cHS4 core can be influenced by distance, and identify an extended core element that confers full barrier activity in the setting of two different classes of retroviral vectors. C1 Univ Washington, Dept Med, Div Med Genet, Seattle, WA 98195 USA. Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USA. NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. RP Emery, DW (reprint author), Univ Washington, Dept Med, Div Med Genet, Box 357720,HSB K236F,1705 NE Pacific, Seattle, WA 98195 USA. EM demery@u.washington.edu FU Intramural NIH HHS NR 26 TC 66 Z9 66 U1 0 U2 5 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1043-0342 J9 HUM GENE THER JI Hum. Gene Ther. PD APR PY 2007 VL 18 IS 4 BP 333 EP 343 DI 10.1089/hum.2007.021 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA 163JN UT WOS:000246156000004 PM 17411365 ER PT J AU Lan, Q Zheng, TZ Shen, M Zhang, YW Wang, SS Zahm, SH Holford, TR Leaderer, B Boyle, P Chanock, S AF Lan, Qing Zheng, Tongzhang Shen, Min Zhang, Yawei Wang, Sophia S. Zahm, Shelia H. Holford, Theodore R. Leaderer, Brian Boyle, Peter Chanock, Stephen TI Genetic polymorphisms in the oxidative stress pathway and susceptibility to non-Hodgkin lymphoma SO HUMAN GENETICS LA English DT Article DE non-Hodgkin lymphoma; oxidative stress; genetic polymorphism ID TYPE-2 DIABETIC-PATIENTS; NAD(P)H OXIDASE P22PHOX; NADPH OXIDASE; MOLECULAR EPIDEMIOLOGY; C242T POLYMORPHISM; DNA-DAMAGE; KAPPA-B; RISK; INFLAMMATION; NEPHROPATHY AB Oxidative damage caused by reactive oxygen species (ROS) and other free radicals is involved in a number of pathological conditions including cancer. In a population-based case-control study of non-Hodgkin lymphoma (NHL) (n = 518 cases, 597 controls) among women in Connecticut, we analyzed one or more single nucleotide polymorphisms (SNPs) in ten candidate genes (AKR1A1, AKR1C1, AKR1C3, CYBA, GPX1, MPO, NOS2A, NOS3, OGG1, and SOD2) that mediate oxidative stress directly or indirectly in the NADPH oxidase-dependent respiratory burst. Odds ratios (OR) and 95% confidence intervals (CI) were adjusted for age and race. Polymorphisms in AKR1A1 and CYBA were significantly associated with increased risk of NHL. There was a 1.7-fold (95% CI = 1.2-2.4, P = 0.0047) increased risk of NHL for individuals who were variant homozygous for the AKR1A1 (IVS5 + 282T > C) SNP. The effect was most pronounced for risk of diffuse large B-cell lymphoma, but risk estimates were non-significantly elevated for other common B-cell histologies and T-cell lymphomas as well. In addition, individuals variant homozygous for the CYBA (Ex4 + 11C > T) SNP had a 1.6-fold (95% CI = 1.1-2.4, P = 0.019) increased risk of NHL that was particularly pronounced for T-cell lymphoma (OR = 3.5, 95% CI = 1.3-9.6, P = 0.013), but was also associated with non-significant increased risks for each of the common B-cell histologies. These results suggest that SNPs in genes related to the oxidative stress pathway may be associated with increased risk of NHL. C1 NCI, Occupat & Environm Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,DHHS,MSC 7240, Bethesda, MD 20892 USA. Yale Sch Med, Dept Epidemiol & Publ Hlth, New Haven, CT USA. Int Agcy Res Canc, F-69372 Lyon, France. NCI, Pedait Oncol Branch, Ctr Canc Res, NIH,DHHS, Bethesda, MD USA. RP Lan, Q (reprint author), NCI, Occupat & Environm Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,DHHS,MSC 7240, 6120 Execut Blvd,EPS 8109, Bethesda, MD 20892 USA. EM qingl@mail.nih.gov RI Boyle, Peter/A-4380-2014; Zahm, Shelia/B-5025-2015 OI Boyle, Peter/0000-0001-6251-0610; FU Intramural NIH HHS; NCI NIH HHS [CA62006] NR 44 TC 35 Z9 37 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 0340-6717 J9 HUM GENET JI Hum. Genet. PD APR PY 2007 VL 121 IS 2 BP 161 EP 168 DI 10.1007/s00439-006-0288-9 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA 145TT UT WOS:000244888700002 PM 17149600 ER PT J AU Figueroa, JD Malats, N Real, FX Silverman, D Kogevinas, M Chanock, S Welch, R Dosemeci, M Tardon, A Serra, C Carrato, A Garcia-Closas, R Castano-Vinyals, G Rothman, N Garcia-Closas, M AF Figueroa, Jonine D. Malats, Nuria Real, Francisco X. Silverman, Debra Kogevinas, Manolis Chanock, Stephen Welch, Robert Dosemeci, Mustafa Tardon, Adonina Serra, Consol Carrato, Alfredo Garcia-Closas, Reina Castano-Vinyals, Gemma Rothman, Nathaniel Garcia-Closas, Montserrat TI Genetic variation in the base excision repair pathway and bladder cancer risk SO HUMAN GENETICS LA English DT Article ID OXIDATIVE DNA-DAMAGE; XRCC1 POLYMORPHISMS; CIGARETTE-SMOKING; CELL-CYCLE; SUSCEPTIBILITY; GENOTYPE; INCREASE; BIAS AB Genetic polymorphisms in DNA repair genes may impact individual variation in DNA repair capacity and alter cancer risk. In order to examine the association of common genetic variation in the base-excision repair (BER) pathway with bladder cancer risk, we analyzed 43 single nucleotide polymorphisms (SNPs) in 12 BER genes (OGG1, MUTYH, APEX1, PARP1, PARP3, PARP4, XRCC1, POLB, POLD1, PCNA, LIG1, and LIG3). Using genotype data from 1,150 cases of urinary bladder transitional cell carcinomas and 1,149 controls from the Spanish Bladder Cancer Study we estimated odds ratios (ORs) and 95% confidence intervals (CIs) adjusting for age, gender, region and smoking status. SNPs in three genes showed significant associations with bladder cancer risk: the 8-oxoG DNA glycosylase gene (OGG1), the Poly (ADP-ribose) polymerase family member 1 (PARP1) and the major gap filling polymerase-beta (POLB). Subjects who were heterozygous or homozygous variant for an OGG1 SNP in the promoter region (rs125701) had significantly decreased bladder cancer risk compared to common homozygous: OR (95%CI) 0.78 (0.63-0.96). Heterozygous or homozygous individuals for the functional SNP PARP1 rs1136410 (V762A) or for the intronic SNP POLB rs3136717 were at increased risk compared to those homozygous for the common alleles: 1.24 (1.02-1.51) and 1.30 (1.04-1.62), respectively. In summary, data from this large case-control study suggested bladder cancer risk associations with selected BER SNPs, which need to be confirmed in other study populations. C1 NCI, Div Canc Epidemiol & Genet, Dept Hlth & Human Serv, Bethesda, MD USA. IMIM, Ctr Res Environm Epidemiol, Barcelona, Spain. Univ Pompeu Fabra, Barcelona, Spain. Sch Med, Iraklion, Greece. NCI, Dept Hlth & Human Serv, Core Genotype Facil, Ctr Adv Technol, Bethesda, MD USA. Univ Oviedo, Oviedo, Spain. Hosp Univ Elche, Elche, Spain. Hosp Univ Canarias, Unidad Invest, San Cristobal la Laguna, Spain. RP Figueroa, JD (reprint author), NCI, Div Canc Epidemiol & Genet, Dept Hlth & Human Serv, Bethesda, MD USA. EM figueroaj@mail.nih.gov RI Serra, C/E-6879-2014; Garcia-Closas, Montserrat /F-3871-2015; Kogevinas, Manolis/C-3918-2017; Real, Francisco X/H-5275-2015; OI Serra, C/0000-0001-8337-8356; Garcia-Closas, Montserrat /0000-0003-1033-2650; Real, Francisco X/0000-0001-9501-498X; Castano-Vinyals, Gemma/0000-0003-4468-1816; Malats, Nuria/0000-0003-2538-3784 FU Intramural NIH HHS NR 29 TC 87 Z9 89 U1 1 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 0340-6717 J9 HUM GENET JI Hum. Genet. PD APR PY 2007 VL 121 IS 2 BP 233 EP 242 DI 10.1007/s00439-006-0294-y PG 10 WC Genetics & Heredity SC Genetics & Heredity GA 145TT UT WOS:000244888700009 PM 17203305 ER PT J AU Pakkanen, S Baffoe-Bonnie, AB Matikainen, MP Koivisto, PA Tammela, TLJ Deshmukh, S Ou, L Bailey-Wilson, JE Schleutker, J AF Pakkanen, Sanna Baffoe-Bonnie, Agnes B. Matikainen, Mika P. Koivisto, Pasi A. Tammela, Teuvo L. J. Deshmukh, Snehal Ou, Liang Bailey-Wilson, Joan E. Schleutker, Johanna TI Segregation analysis of 1,546 prostate cancer families in Finland shows recessive inheritance SO HUMAN GENETICS LA English DT Article ID AUTOSOMAL-DOMINANT INHERITANCE; SUSCEPTIBILITY LOCUS; GERMLINE MUTATIONS; PEDIGREE DATA; HEREDITARY; LINKAGE; GENE; DISEASE; RISK; ONSET AB Prostate cancer (PCa) is the most frequently diagnosed cancer in men worldwide and is likely to be caused by a number of genes with different modes of inheritance, population frequencies and penetrance. The objective of this study was to assess the familial aggregation of PCa in a sample of 1,546 nuclear families ascertained through an affected father and diagnosed during 1988-1993, from the unique, founder population-based resource of the Finnish Cancer Registry. Segregation analysis was performed for two cohorts of 557 early-onset and 989 late-onset families evaluating residual paternal effects and assuming that age at diagnosis followed a logistic distribution after log-transformation. The results did not support an autosomal dominant inheritance as has been reported in many of the hospital-based prostatectomy series. Instead, it confirmed the existence of hereditary PCa in the Finnish population under a complex model that included a major susceptibility locus with Mendelian recessive inheritance and a significant paternal regressive coefficient that is indicative of a polygenic/multifactorial component. The strengths of our study are the homogenous Finnish population, large epidemiological population-based data, histologically confirmed cancer diagnosis done before the PSA-era in Finland and registry based approach. Our results support the evidence that the inheritance of PCa is controlled by major genes and are in line with the previous linkage studies. Moreover, this is the first time a recessive inheritance is suggested to fit PCa in all data even when divided to early and late-onset cohorts. C1 Univ Tampere, Canc Genet Lab, Inst Med Technol, Tampere 33014, Finland. Tampere Univ Hosp, Tampere 33014, Finland. Fox Chase Canc Ctr, Div Populat Sci, Philadelphia, PA 19111 USA. NHGRI, NIH, Baltimore, MD 21131 USA. Tampere Univ, Sch Med, Tampere, Finland. Tampere Univ Hosp, Dept Urol, Tampere, Finland. RP Schleutker, J (reprint author), Univ Tampere, Canc Genet Lab, Inst Med Technol, Biokatu 8, Tampere 33014, Finland. EM Johanna.Schleutker@uta.fi OI Bailey-Wilson, Joan/0000-0002-9153-2920 FU Intramural NIH HHS; NCI NIH HHS [CA-06927, P30 CA006927]; NCRR NIH HHS [P41 RR003655, RR03655]; NHGRI NIH HHS [Z01 HG000107-09] NR 46 TC 17 Z9 17 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 0340-6717 J9 HUM GENET JI Hum. Genet. PD APR PY 2007 VL 121 IS 2 BP 257 EP 267 DI 10.1007/s00439-006-0310-2 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA 145TT UT WOS:000244888700011 PM 17203302 ER PT J AU Zhang, ZJ Lee, YC Kim, SJ Choi, MS Tsai, PC Saha, A Wei, H Xu, Y Xiao, YJ Zhang, P Heffer, A Mukherjee, AB AF Zhang, Zhongjian Lee, Yi-Ching Kim, Sung-Jo Choi, Moonsuk S. Tsai, Pei-Chih Saha, Arjun Wei, Hui Xu, Yan Xiao, Yi-Jin Zhang, Peng Heffer, Alison Mukherjee, Anil B. TI Production of lysophosphatidylcholine by cPLA(2) in the brain of mice lacking PPT1 is a signal for phagocyte infiltration SO HUMAN MOLECULAR GENETICS LA English DT Article ID NEURONAL CEROID-LIPOFUSCINOSIS; UNFOLDED PROTEIN RESPONSE; ENDOPLASMIC-RETICULUM; APOPTOTIC CELLS; ACTIVATION; MICROGLIA; INCL; NEURODEGENERATION; DEATH; NEUROTOXICITY AB In the majority of neurodegenerative storage disorders, neuronal death in the brain is followed by infiltration of phagocytic cells (e.g. activated microglia, astroglia and macrophages) for the efficient removal of cell corpses. However, it is increasingly evident that these phagocytes may also cause death of adjoining viable neurons contributing to rapid progression of neurodegeneration. Infantile neuronal ceroid lipofuscinosis (INCL) is a devastating, neurodegenerative, lysosomal storage disorder caused by inactivating mutations in the palmitoyl-protein thioesterase-1 (PPT1) gene. PPT1 catalyzes the cleavage of thioester linkages in S-acylated (palmitoylated) proteins and its deficiency leads to abnormal accumulation of thioesterified polypeptides (ceroid) in lysosomes causing INCL pathogenesis. PPT1-knockout (PPT1-KO) mice mimic the clinical and pathological features of human INCL including rapid neuronal death by apoptosis and phagocyte infiltration. We previously reported that in PPT1-KO mice, the neurons undergo endoplasmic reticulum stress activating unfolded protein response, which mediates caspase-12 activation and apoptosis. However, the molecular mechanism(s) by which the phagocytic cells are recruited in the PPT1-KO mouse brain remains poorly understood. We report here that increased production of lysophosphatidylcholine (LPC), catalyzed by the activation of cytosolic phospholipase A(2) (cPLA(2)) in the PPT1-KO mouse brain, is a 'lipid signal' for phagocyte recruitment. We also report that an age-dependent increase in LPC levels in the PPT1-KO mouse brain positively correlates with elevated expression of the genes characteristically associated with phagocytes. We propose that increased cPLA(2)-catalyzed LPC production in the brain is at least one of the mechanisms that mediate phagocyte infiltration contributing to INCL neuropathology. C1 NICHHD, Sect Dev Genet, Heritable Disorders Branch, NIH, Bethesda, MD 20892 USA. Cleveland Clin Fdn, Dept Canc Biol, Lerner Res Inst, Cleveland, OH 44195 USA. Walter Reed Army Inst Res, Silver Spring, MD 20910 USA. RP Mukherjee, AB (reprint author), NICHHD, Sect Dev Genet, Heritable Disorders Branch, NIH, Bldg 10,Room 9D42,10 Ctr Dr, Bethesda, MD 20892 USA. EM mukherja@exchange.nih.gov FU Intramural NIH HHS; NCI NIH HHS [R01-CA89228] NR 44 TC 19 Z9 19 U1 2 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 J9 HUM MOL GENET JI Hum. Mol. Genet. PD APR 1 PY 2007 VL 16 IS 7 BP 837 EP 847 DI 10.1093/hmg/ddm029 PG 11 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 162XI UT WOS:000246122400011 PM 17341491 ER PT J AU Bar, H Goudeau, B Walde, S Casteras-Simon, M Mucke, N Shatunov, A Goldberg, YP Clarke, C Holton, JL Eymard, B Katus, HA Fardeau, M Goldfarb, L Vicart, P Herrmann, H AF Bar, Harald Goudeau, Bertrand Walde, Sarah Casteras-Simon, Monique Mucke, Norbert Shatunov, Alexey Goldberg, Y. Paul Clarke, Charles Holton, Janice L. Eymard, Bruno Katus, Hugo A. Fardeau, Michel Goldfarb, Lev Vicart, Patrick Herrmann, Harald TI Conspicuous involvement of desmin tail mutations in diverse cardiac and skeletal myopathies SO HUMAN MUTATION LA English DT Article DE myofibrillar myopathy (MFM); cardiomyopathy; desminopathy; DES; intermediate filament protein ID INTERMEDIATE-FILAMENT PROTEIN; EPIDERMOLYSIS-BULLOSA SIMPLEX; ALPHA-B-CRYSTALLIN; IN-VITRO; MYOFIBRILLAR MYOPATHY; MUSCULAR-DYSTROPHY; MITOCHONDRIAL DYSFUNCTION; ASSEMBLY PROPERTIES; AUTOSOMAL-DOMINANT; MISSENSE MUTATIONS AB Myofibrillar myopathy (MFM) encompasses a genetically heterogeneous group of human diseases caused by mutations in genes coding for structural proteins of muscle. Mutations in the intermediate filament (IF) protein desmin (DES), a major cytoskeletal component of myocytes, lead to severe forms of "desminopathy," which affects cardiac, Skeletal, and smooth muscle. Most mutations described reside in the central (X,helical rod domain of desmin. Here we report three novel mutations-c.1325C > T (p.T4421), c.1360C > T (p.R454W), and c.1379G > T (p.S4601)-located in desmin's non,a-helical carboxy-terminal "tail" domain. We have investigated the impact of these and four-c.1237G > A (p.E413K), C.1346A > C (p.K449T), c.1353C > G (p.1451M), and c.1405G > A (p.V469M) -previously described "tail" mutations on in vitro filament formation and on the generation of ordered cytoskeletal arrays in transfected myoblasts. Although all but two mutants (p.E413K, p.R454W) assembled into IFs in vitro and all except p.E413K were incorporated into IF arrays in transfected C2C12 cells, filament properties differed significantly from wild,type desmin as revealed by viscometric assembly assays. Most notably, when coassembled with wild-type desmin, these mutants revealed a severe disturbance of filament-formation competence and filament-filament interactions, indicating an inherent incompatibility of mutant and wild,type protein to form mixed filaments. The various clinical phenotypes observed may reflect altered interactions of desmin's tail domain with different components of the myoblast cytoskeleton leading to diminished biomechanical properties and/or altered metabolism of the individual myocyte. Our in vitro assembly regimen proved to be a very sensible tool to detect if a particular desmin mutation is able to cause filament abnormalities. C1 German Canc Res Ctr, Dept Mol Genet, D-69120 Heidelberg, Germany. Univ Heidelberg, Dept Cardiol, D-6900 Heidelberg, Germany. Univ Paris 07, UFR Biochim, EA300, F-75221 Paris, France. German Canc Res Ctr, Div Biophys Macromol, D-69120 Heidelberg, Germany. NINDS, NIH, Bethesda, MD 20892 USA. Univ British Columbia, Dept Med Genet, Vancouver, BC V5Z 1M9, Canada. UCL Natl Hosp Neurol & Neurosurg, London WC1N 3BG, England. Inst Neurol, Dept Neuropathol, London WC1N 3BG, England. Hop La Pitie Salpetriere, Inst Myol, Paris, France. RP Herrmann, H (reprint author), German Canc Res Ctr, Dept Mol Genet, Technol Pk 3,Neuenheimer Feld 580, D-69120 Heidelberg, Germany. EM h.herrmann@dkfz.de RI Shatunov, Aleksey/E-6946-2011; Katus, Hugo/P-1712-2016; Holton, Janice/F-6831-2011 OI Holton, Janice/0000-0002-3882-5249 FU Intramural NIH HHS NR 76 TC 48 Z9 53 U1 2 U2 6 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 1059-7794 J9 HUM MUTAT JI Hum. Mutat. PD APR PY 2007 VL 28 IS 4 BP 374 EP 386 DI 10.1002/humu.20459 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA 148XL UT WOS:000245110600008 PM 17221859 ER PT J AU Cabral, WA Makareeva, E Letocha, AD Scribanu, N Fertala, A Steplewski, A Keene, DR Persikov, AV Leikin, S Marini, JC AF Cabral, Wayne A. Makareeva, Elena Letocha, Anne D. Scribanu, Nina Fertala, Andrzej Steplewski, Andrzej Keene, Douglas R. Persikov, Anton V. Leikin, Sergey Marini, Joan C. TI Y-position cysteine substitution in type I collagen (alpha 1(I) R888C/p.R1066C) is associated with osteogenesis imperfecta/Ehlers-Danlos syndrome phenotype SO HUMAN MUTATION LA English DT Article DE osteogenesis imperfecta (OI); Ehlers-Danlos syndrome (EDS); N-propeptide processing; COL1A1; collagen kinking ID TRIPLE-HELICAL STRUCTURE; AMINO-ACID SUBSTITUTIONS; PROCOLLAGEN GENE COL2A1; SPONDYLOEPIPHYSEAL DYSPLASIA; PRECOCIOUS OSTEOARTHRITIS; MOLECULAR PACKING; N-PROTEINASE; CYS SUBSTITUTION; TALL STATURE; MUTATION AB The most common mutations in type I collagen causing types II-IV osteogenesis imperfecta (01) result in substitution for glycine in a Gly-Xaa-Yaa triplet by another amino acid. We delineated a Y-position substitution in a small pedigree with a combined OI/Ehlers-Danlos Syndrome (EDS) phenotype, characterized by moderately decreased DEYA z-score (-1.3 to -2.6), long bone fractures, and large,joint hyperextensibility. Affected individuals have an alpha 1(I)R888C (p.R1066C) substitution in one COL1A1 allele. Polyacrylamide gel electrophoresis (PAGE) of [H-3]-proline labeled steady,state collagen reveals slight overmodification of the a 1 (1) monomer band, much less than expected for a substitution of a neighboring glycine residue, and a faint alpha 1(1) dimer. Dimers form in about 10% of proband type I collagen. Dimer formation is inefficient compared to a possible 25%, probably because the SH-side chains have less proximity in this Y-position than when substituting for a glycine. Theoretical stability calculations, differential scanning calorimetry (DSC) thermograms, and thermal denaturation curves showed only weak local destabilization from the Y-position substitution in one or two chains of a collagen helix, but greater destabilization is seen in collagen containing dimers. Y-position collagen dimers cause kinking of the helix, resulting in a register shift that is propagated the full length of the helix and causes resistance to procollagen processing by N proteinase. Collagen containing the Y-position substitution is incorporated into matrix deposited in culture, including immaturely and maturely cross-linked fractions. In vivo, proband dermal fibrils have decreased density and increased diameter compared to controls, with occasional aggregate formation. This report on Y-position substitutions in type I collagen extends the range of phenotypes caused by nonglycine substitutions and shows that, similar to X- and Y-position substitutions in types II and III collagen, the phenotypes resulting from nonglycine substitutions in type I collagen are distinct from those caused by glycine substitutions. C1 NICHHD, Bone & Extracellular Matrix Branch, NIH, Bethesda, MD 20892 USA. NICHHD, Sect Phys Biochem, NIH, Bethesda, MD 20892 USA. Georgetown Univ, Med Ctr, Dept Pediat, Washington, DC 20007 USA. Thomas Jefferson Univ, Jefferson Med Coll, Dept Dermatol & Cutaneous Biol, Philadelphia, PA 19107 USA. Oregon Hlth & Sci Univ, Shriners Hosp Children, Portland, OR 97201 USA. Oregon Hlth & Sci Univ, Dept Mol & Med Genet, Portland, OR 97201 USA. Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Biochem, Piscataway, NJ 08854 USA. RP Marini, JC (reprint author), NICHHD, Bone & Extracellular Matrix Branch, NIH, Bldg 10,Rm 10N260,9000 Rockville Pike, Bethesda, MD 20892 USA. EM oidoc@helix.nih.gov RI Leikin, Sergey/A-5518-2008; Makareeva, Elena/F-5183-2011 OI Leikin, Sergey/0000-0001-7095-0739; FU NIAMS NIH HHS [R01AR48544] NR 48 TC 27 Z9 29 U1 0 U2 2 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 1059-7794 J9 HUM MUTAT JI Hum. Mutat. PD APR PY 2007 VL 28 IS 4 BP 396 EP 405 DI 10.1002/humu.20456 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA 148XL UT WOS:000245110600010 PM 17206620 ER PT J AU Zhu, JL Basso, O Obel, C Christensen, K Olsen, J AF Zhu, Jin Liang Basso, Olga Obel, Carsten Christensen, Kaare Olsen, Jorn TI Infertility, infertility treatment and twinning: the Danish National Birth Cohort SO HUMAN REPRODUCTION LA English DT Article DE infertility; infertility treatment; time to pregnancy; twinning; zygosity ID QUESTIONNAIRE; PREGNANCIES; ZYGOSITY; REGISTRY; RATES; TWINS AB BACKGROUND: We have previously observed that an increasing time to pregnancy (TTP) is associated with a reduced frequency of twin deliveries in couples not receiving infertility treatment. By using updated information, we assessed the frequencies of dizygotic (DZ) and monozygotic (MZ) twin deliveries as a function of infertility (TTP > 12 months), as well as infertility treatment. METHODS: From the Danish National Birth Cohort (19972003), we identified 51 730 fertile couples with TTP <= 12 months, 5838 infertile couples who conceived naturally with TTP > 12 months and 5163 infertile couples who conceived after treatment. Information on zygosity, available for part of the cohort (1997-2000), was based on standardized questions on the similarities between the twins at the age of 3-5 years. RESULTS: Compared with fertile couples, the frequency of DZ twin deliveries was lower for infertile couples conceiving naturally (odds ratio 0.4, 95% confidence interval 0.2-0.7) and was much higher for infertile couples conceiving after treatment (17.3, 14.4-20.7). The frequency of DZ twin deliveries decreased with TTP in untreated couples, whereas the frequency of MZ twin deliveries remained constant. CONCLUSIONS: The frequency of DZ twin deliveries decreased with TTP and substantially increased with infertility treatment, whereas MZ twin deliveries remained substantially unchanged. C1 Aarhus Univ, Inst Publ Hlth, Danish Epidemiol Sci Ctr, Dept Epidemiol, DK-8000 Aarhus C, Denmark. NIEHS, Epidemiol Branch, Dept Hlth & Human Serv, NIH, Res Triangle Pk, NC 27709 USA. Aarhus Univ Hosp, Perinatal Epidemiol Res Unit, Dept Obstet & Gynaecol, Aarhus N, Denmark. Univ So Denmark, Inst Publ Hlth, Ctr Prevent Congenital Malformat, Odense, Denmark. Univ Calif Los Angeles, Sch Publ Hlth, Dept Epidemiol, Los Angeles, CA 90024 USA. RP Zhu, JL (reprint author), Aarhus Univ, Inst Publ Hlth, Danish Epidemiol Sci Ctr, Dept Epidemiol, Vennelyst Blvd 6, DK-8000 Aarhus C, Denmark. EM zjl@soci.au.dk RI Basso, Olga/E-5384-2010; Christensen, Kaare/C-2360-2009; Olsen, Jorn/F-8801-2015 OI Basso, Olga/0000-0001-9298-4921; Christensen, Kaare/0000-0002-5429-5292; Olsen, Jorn/0000-0001-7462-5140 FU Intramural NIH HHS [Z99 ES999999] NR 19 TC 15 Z9 15 U1 1 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0268-1161 J9 HUM REPROD JI Hum. Reprod. PD APR PY 2007 VL 22 IS 4 BP 1086 EP 1090 DI 10.1093/humrep/del495 PG 5 WC Obstetrics & Gynecology; Reproductive Biology SC Obstetrics & Gynecology; Reproductive Biology GA 162TB UT WOS:000246111300028 PM 17204529 ER PT J AU Newton-Cheh, C Guo, CY Gona, P Larson, MG Benjamin, EJ Wang, TJ Kathiresan, S O'Donnell, CJ Musone, SL Camargo, AL Drake, JA Levy, D Hirschhorn, JN Vasan, RS AF Newton-Cheh, Christopher Guo, Chao-Yu Gona, Philimon Larson, Martin G. Benjamin, Emelia J. Wang, Thomas J. Kathiresan, Sekar O'Donnell, Christopher J. Musone, Stacy L. Camargo, Amy L. Drake, Jared A. Levy, Daniel Hirschhorn, Joel N. Vasan, Ramachandran S. TI Clinical and genetic correlates of aldosterone-to-renin ratio and relations to blood pressure in a community sample SO HYPERTENSION LA English DT Article DE aldosterone; renin; hypertension, essential; blood pressure; genetics; population; risk factors ID HORMONE REPLACEMENT THERAPY; HYPERALDOSTERONISM TYPE-II; TRAIT LINKAGE ANALYSIS; ESSENTIAL-HYPERTENSION; SERUM ALDOSTERONE; ACTIVE RENIN; POSTMENOPAUSAL WOMEN; ANGIOTENSIN SYSTEM; PLASMA-ALDOSTERONE; SODIUM-INTAKE AB Aldosterone: renin ratio (ARR) is used to screen for hyperaldosteronism. Data regarding correlates of ambulatory ARR in the community and its relation to hypertension incidence are limited. We defined clinical correlates of ARR, determined its heritability, tested for association and linkage, and related ARR to blood pressure (BP) progression in nonhypertensive individuals among 3326 individuals from the Framingham Heart Study (53% women; mean age: 59 years). Ambulatory morning ARR (serum aldosterone and plasma renin concentrations) were related to clinical covariates, genetic variation across the REN locus, a 10-cM linkage map, and among nonhypertensive participants (n = 1773) to progression of >= 1 Sixth Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure BP category (optimal: < 120/80 mm Hg, normal: 120 to 129/80 to 84 mm Hg, high normal: 130 to 139/85 to 89 mm Hg, hypertension: >= 140/90 mm Hg), or incident hypertension (systolic BP: >= 140 mm Hg, diastolic BP: >= 90 mm Hg, or use of antihypertensive treatment). ARR was positively associated with age, female sex, untreated hypertension, total/high-density lipoprotein cholesterol ratio, hormone replacement therapy, and beta-blocker use, but negatively associated with angiotensin-converting enzyme inhibitor and diuretic use. ARR was heritable (h(2) = 0.40), had modest linkage to chromosome 11p (logarithm of the odds: 1.89), but was not associated with 17 common variants in REN (n = 1729). On follow-up (mean: 3 years), 607 nonhypertensive individuals (34.2%) developed BP progression, and 283 (16.0%) developed hypertension. Higher baseline logARR was associated with increased risk of BP progression (odds ratio per SD increment: 1.23; 95% CI: 1.11 to 1.37) and hypertension incidence (odds ratio per SD increment: 1.16; 95% CI: 1.00 to 1.33). ARR is a heritable trait influenced by clinical and genetic factors. There is a continuous gradient of increasing risk of BP progression across ARR levels in nonhypertensive individuals. C1 NHLBI, Framingham Heart Dis Epidemiol Study, Framingham, MA 01702 USA. Boston Univ, Sch Med, Framingham, MA 01702 USA. Harvard Univ, Broad Inst, Cambridge, MA 02138 USA. MIT, Cambridge, MA 02139 USA. Massachusetts Gen Hosp, Div Cardiol, Boston, MA 02114 USA. Boston Univ, Dept Math & Stat, Boston, MA 02215 USA. Boston Univ, Sch Med, Sect Epidemiol & Prevent Med, Boston, MA 02215 USA. Boston Univ, Sch Med, Evans Dept Med, Boston, MA 02215 USA. Boston Univ, Sch Med, Whitaker Cardiovasc Inst, Boston, MA 02215 USA. Childrens Hosp, Boston, MA 02115 USA. RP Newton-Cheh, C (reprint author), NHLBI, Framingham Heart Dis Epidemiol Study, 73 Mt Wayte Ave,Suite 2, Framingham, MA 01702 USA. EM cnewtoncheh@partners.org OI Larson, Martin/0000-0002-9631-1254; Ramachandran, Vasan/0000-0001-7357-5970; Benjamin, Emelia/0000-0003-4076-2336 FU NHLBI NIH HHS [N01-HC-25195, 1R01HL67288, 2K24 HL04334, K23HL074077, K23HL080025] NR 63 TC 108 Z9 110 U1 1 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0194-911X J9 HYPERTENSION JI Hypertension PD APR PY 2007 VL 49 IS 4 BP 846 EP 856 DI 10.1161/01.HYP.0000258554.87444.91 PG 11 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 148VG UT WOS:000245104300023 PM 17296870 ER PT J AU Rame, JE Drazner, MH Post, W Peshock, R Lima, J Cooper, RS Dries, DL AF Rame, J. Eduardo Drazner, Mark H. Post, Wendy Peshock, Ronald Lima, Joao Cooper, Richard S. Dries, Daniel L. TI Corin I555(p568) allele is associated with enhanced cardiac hypertrophic response to increased systemic afterload SO HYPERTENSION LA English DT Article DE hypertension; left ventricular hypertrophy; natriuretic peptides; genetic polymorphisms; left ventricular remodeling ID LEFT-VENTRICULAR MASS; PROATRIAL NATRIURETIC PEPTIDE; GUANYLYL CYCLASE-A; CARDIOVASCULAR HEALTH; RECEPTOR; PRESSURE; MICE; HYPERTENSION; POPULATION; TWINS AB Corin activates pro-A-type naturetic peptide and pro-B-type naturetic peptide into biologically active molecules. We recently identified a minor allele in the corin gene defined by 2 highly linked single nucleotide polymorphisms (T555I and Q568P), which was associated with hypertension in blacks. Because of the direct antihypertrophic effects of the natriuretic peptide system, we hypothesized that the minor corin I555(P568) allele would be associated with an enhanced hypertrophic response to pressure overload. The relationship between systolic blood pressure and indexed left ventricular mass, derived from cardiac MRI, was analyzed in the Dallas Heart Study as a function of corin allele status. The Multi-Ethnic Study of Atherosclerosis was used as a validation cohort. All of the analyses were limited to self-identified blacks without treatment for hypertension. In addition, we genotyped 2114 markers highly informative for African ancestry in the Dallas Heart Study and derived a covariate representing African ancestry for multivariate models. In adjusted analysis, the corin I555(P568) allele was an independent predictor of left-ventricular mass in subjects with elevated systolic blood pressure. Linear spline regression analysis confirmed a significant interaction (P = 0.002) between the corin I555(P568) allele and systolic blood pressure as a predictor of left ventricular mass in subjects with systolic blood pressure > 120 mm Hg, and this nonlinear interaction was replicated in the Multi-Ethnic Study of Atherosclerosis. In the Dallas Heart Study, the corin I555(P568) allele was also associated with an increased odds for prevalent left ventricular hypertrophy in the presence of untreated hypertension. These data suggest that the corin I555(P568) allele represents a cardiac hypertrophy-sensitizing genetic locus in systemic hypertension. C1 Univ Texas, SW Med Ctr, Donald W Reynolds Cardiovasc Clin Res Ctr, Div Cardiol, Dallas, TX 75230 USA. Univ Calif San Francisco, Div Cardiol, San Francisco, CA 94143 USA. NHLBI, Cardiovasc Branch, Bethesda, MD 20892 USA. Johns Hopkins Univ, Div Cardiol, Donald W Reynolds Cardiovasc Clin Res Ctr, Baltimore, MD 21218 USA. Loyola Univ, Stritch Sch Med, Dept Prevent Med & Epidemiol, Maywood, IL 60153 USA. Hosp Univ Penn, Div Cardiovasc, Philadelphia, PA 19104 USA. RP Dries, DL (reprint author), Univ Penn, Div Cardiovasc Med, 6 Penn Tower,3400 Spruce St, Philadelphia, PA 19104 USA. EM daniel.dries@uphs.upenn.edu FU NHLBI NIH HHS [N01-HC-95169, K23-HL04455, N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC-95162, N01-HC-95163, N01-HC-95164, N01-HC-95165]; PHS HHS [R0-1 45508, R0-1 47910] NR 37 TC 73 Z9 76 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0194-911X J9 HYPERTENSION JI Hypertension PD APR PY 2007 VL 49 IS 4 BP 857 EP 864 DI 10.1161/01.HYP.0000258566.95867.9e PG 8 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 148VG UT WOS:000245104300024 PM 17296875 ER PT J AU Long, EO AF Long, Eric O. TI Ready for prime time: NK cell priming by dendritic cells SO IMMUNITY LA English DT Editorial Material ID NATURAL-KILLER-CELLS; MHC CLASS-I; TOLERANCE; RESPONSES; SELF AB Natural killer (NK) cells were long thought to respond directly to infected cells and tumor cells. In this issue of Immunity, Lucas et al. (2007) repeal this view by showing that NK cells acquire functionality through priming by dendritic cells. C1 NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. RP Long, EO (reprint author), NIAID, Immunogenet Lab, NIH, 12441 Parklawn Dr, Rockville, MD 20852 USA. EM elong@nih.gov RI Long, Eric/G-5475-2011 OI Long, Eric/0000-0002-7793-3728 NR 10 TC 23 Z9 28 U1 1 U2 4 PU CELL PRESS PI CAMBRIDGE PA 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA SN 1074-7613 J9 IMMUNITY JI Immunity PD APR PY 2007 VL 26 IS 4 BP 385 EP 387 DI 10.1016/j.immuni.2007.04.001 PG 3 WC Immunology SC Immunology GA 162YW UT WOS:000246126400002 PM 17459805 ER PT J AU Xue, HH Bollenbacher-Reilley, J Wu, Z Spolski, R Jing, X Zhang, YC McCoy, JP Leonard, WJ AF Xue, Hai-Hui Bollenbacher-Reilley, Julie Wu, Zheng Spolski, Rosanne Jing, Xuefang Zhang, Yi-Chen McCoy, J. Philip Leonard, Warren J. TI The transcription factor GABP is a critical regulator of B lymphocyte development SO IMMUNITY LA English DT Article ID CELL-DEVELOPMENT; MARGINAL-ZONE; CHAIN GENE; INTERLEUKIN-7 RECEPTOR; IMMUNOGLOBULIN-BETA; PAX-5 BSAP; FACTOR EBF; IGH LOCUS; B1B CELLS; DIFFERENTIATION AB GA binding protein (GABP) is a ubiquitously expressed Ets-family transcription factor that critically regulates the expression of the interleukin-7 receptor alpha chain (IL-7R alpha) in T cells, whereas it is dispensable for IL-7R alpha expression in fetal liver B cells. Here we showed that deficiency of GABP alpha, the DNA-binding subunit of GABP, resulted in profoundly defective B cell development and a compromised humoral immune response, in addition to thymic developmental defects. Furthermore, the expression of Pax5 and Pax5 target genes such as Cd79a was greatly diminished in GABP alpha-deficient B cell progenitors, pro-B, and mature B cells. GABP could bind to the regulatory regions of Pax5 and Cd79a in vivo. Thus, GABP is a key regulator of B cell development, maturation, and function. C1 NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. NHLBI, Flow Cytometry Core Facil, NIH, Bethesda, MD 20892 USA. Univ Iowa, Carver Coll Med, Dept Microbiol, Iowa City, IA 52242 USA. RP Leonard, WJ (reprint author), NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. EM wjl@helix.nih.gov FU Intramural NIH HHS NR 41 TC 33 Z9 33 U1 1 U2 3 PU CELL PRESS PI CAMBRIDGE PA 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA SN 1074-7613 J9 IMMUNITY JI Immunity PD APR PY 2007 VL 26 IS 4 BP 421 EP 431 DI 10.1016/j.immuni.2007.03.010 PG 11 WC Immunology SC Immunology GA 162YW UT WOS:000246126400008 PM 17442597 ER PT J AU Qiao, M Thornton, AM Shevach, EM AF Qiao, Miao Thornton, Angela M. Shevach, Ethan M. TI CD4(+) CD5(+) regulatory T cells render naive CD4(+) CD25(-)T cells anergic and suppressive SO IMMUNOLOGY LA English DT Article DE regulatory T cells; T-cell activation; tolerance; suppression; anergy ID TRANSCRIPTION FACTOR FOXP3; RECEPTOR TRANSGENIC MICE; CUTTING EDGE; IN-VITRO; HELPER-CELLS; ANTIGEN; ACTIVATION; INDUCTION; IL-2; INTERLEUKIN-4 AB CD4(+) CD25(+) Foxp3(+) naturally occurring regulatory T cells (nTreg) are potent inhibitors of almost all immune responses. However, it is unclear how this minor population of cells is capable of exerting its powerful suppressor effects. To determine whether nTreg mediate part of their suppressor function by rendering naive T cells anergic or by converting them to the suppressor phenotype, we cocultured mouse nTreg with naive CD4(+) CD25(-) T cells from T-cell receptor (TCR) transgenic mice on a RAG deficient (RAG(-/-)) background in the presence of anti-CD3 and interleukin-4 (IL-4) to promote cell viability. Two distinct responder cell populations could be recovered from the cocultures. One population remained undivided in the coculture and was non-responsive to restimulation with anti-CD3 or exogenous IL-2, and could not up-regulate IL-2 mRNA or CD25 expression upon TCR restimulation. Those responder cells that had divided in the coculture were anergic to restimulation with anti-CD3 but responded to restimulation with IL-2. The undivided population was capable of suppressing the response of fresh CD4(+) CD25(-) T cells and CD8(+) T cells, while the divided population was only marginally suppressive. Although cell contact between the induced regulatory T cell (iTreg) and the responders was required for suppression to be observed, anti-transforming growth factor-beta partially abrogated their suppressive function. The iTreg did not express Foxp3. Therefore nTreg are not only able to suppress immune responses by inhibiting cytokine production by CD4(+) CD25(-) responder cells, but also appear to modulate the responder cells to render them both anergic and suppressive. C1 NIAID, Cellular Immunol Sect, Immunol Lab, NIH, Bethesda, MD 20892 USA. RP Shevach, EM (reprint author), NIAID, Cellular Immunol Sect, Immunol Lab, NIH, Bldg 10 11N315, Bethesda, MD 20892 USA. EM eshevach@niaid.nih.gov NR 30 TC 33 Z9 42 U1 0 U2 1 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0019-2805 J9 IMMUNOLOGY JI Immunology PD APR PY 2007 VL 120 IS 4 BP 447 EP 455 DI 10.1111/j.1365-2567.2007.02544.x PG 9 WC Immunology SC Immunology GA 143KH UT WOS:000244719600002 PM 17244157 ER PT J AU Oakley, MSM Kumar, S Anantharaman, V Zheng, H Mahajan, B Haynes, JD Moch, JK Fairhurst, R McCutchan, TF Aravind, L AF Oakley, Miranda S. M. Kumar, Sanjai Anantharaman, Vivek Zheng, Hong Mahajan, Babita Haynes, J. David Moch, J. Kathleen Fairhurst, Rick McCutchan, Thomas F. Aravind, L. TI Molecular factors and biochemical pathways induced by febrile temperature in intraerythrocytic Plasmodium falciparum parasites SO INFECTION AND IMMUNITY LA English DT Article ID HEAT-SHOCK PROTEINS; MULTIPLE SEQUENCE ALIGNMENT; INFECTED ERYTHROCYTES; ANTIGENIC VARIATION; GROWTH-INHIBITION; MALARIA PARASITES; MOSQUITO MIDGUT; HEMOGLOBIN-C; IN-VITRO; APOPTOSIS AB Intermittent episodes of febrile illness are the most benign and recognized symptom of infection with malaria parasites, although the effects on parasite survival and virulence remain unclear. In this study, we identified the molecular factors altered in response to febrile temperature by measuring differential expression levels of individual genes using high-density oligonucleotide microarray technology and by performing biological assays in asexual-stage Plasmodiuntfalciparurn parasite cultures incubated at 37 degrees C and 41 degrees C (an elevated temperature that is equivalent to malaria-induced febrile illness in the host). Elevated temperature had a profound influence on expression of individual genes; 336 of approximately 5,300 genes (6.3% of the genome) had altered expression profiles. Of these, 163 genes (49%) were upregulated by twofold or greater, and 173 genes (51%) were downregulated by twofold or greater. In-depth sensitive sequence profile analysis revealed that febrile temperature-induced responses caused significant alterations in the major parasite biologic networks and pathways and that these changes are well coordinated and intricately linked. One of the most notable transcriptional changes occurs in genes encoding proteins containing the predicted Pexel motifs that are exported into the host cytoplasm or inserted into the host cell membrane and are likely to be associated with erythrocyte remodeling and parasite sequestration functions. Using our sensitive computational analysis, we were also able to assign biochemical or biologic functional predictions for at least 100 distinct genes previously annotated as "hypothetical." We find that cultivation of P.falciparum parasites at 41 degrees C leads to parasite death in a time-dependent manner. The presence of the "crisis forms" and the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling-positive parasites following heat treatment strongly support the notion that an apoptosis-like cell death mechanism might be induced in response to febrile temperatures. These studies enhance the possibility of designing vaccines and drugs on the basis of disruption in molecules and pathways of parasite survival and virulence activated in response to febrile temperatures. C1 US FDA, Ctr Biol Evaluat & Res, Div Emerging & Transfus Transmitted Dis, Bethesda, MD 20892 USA. NIAID, Lab Malaria & Vector Res, NIH, Rockville, MD USA. Uniformed Serv Univ Hlth Sci, Emerging Infect Dis Program, Bethesda, MD 20814 USA. Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD USA. Walter Reed Army Inst Res, Silver Spring, MD USA. USN, Med Res Ctr, Silver Spring, MD USA. RP Kumar, S (reprint author), US FDA, Ctr Biol Evaluat & Res, Div Emerging & Transfus Transmitted Dis, Bethesda, MD 20892 USA. EM Sanjai.kumar@fda.hhs.gov OI Anantharaman, Vivek/0000-0001-8395-0009 FU Intramural NIH HHS NR 52 TC 69 Z9 70 U1 1 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD APR PY 2007 VL 75 IS 4 BP 2012 EP 2025 DI 10.1128/IAI.01236-06 PG 14 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 153GY UT WOS:000245421400054 PM 17283083 ER PT J AU Dzutsev, AH Belyakov, IM Isakov, DV Margulies, DH Berzofsky, JA AF Dzutsev, Amiran H. Belyakov, Igor M. Isakov, Dmitry V. Margulies, David H. Berzofsky, Jay A. TI Avidity of CD8 T cells sharpens immunodominance SO INTERNATIONAL IMMUNOLOGY LA English DT Article DE immunodominance; cytotoxic; T lymphocytes; avidity; epitope ID MAJOR HISTOCOMPATIBILITY COMPLEX; MHC CLASS-I; ALTERED PEPTIDE LIGAND; LYMPHOCYTES-T; SELECTIVE EXPANSION; MONOCLONAL-ANTIBODY; VACCINE STRATEGIES; VIRAL-INFECTION; ANTIGEN; REPERTOIRE AB In the course of viral infection, the immune system exploits only a fraction of the available CTL repertoire and focuses on a few of a myriad of potentially antigenic peptides. This phenomenon, known as immunodominance, depends on a number of factors, including antigen processing and transport, MHC binding, competition for antigen-presenting cells, availability of the CD8 T cell repertoire and other mechanisms that function largely by restricting the immune response. Here we elucidate a novel mechanism that increases the immunodominance of the epitope rather by enhancing the immune response. Using a peptide-specific MHC-restricted mAb and functional assays of CTL activation, we show that T cells with high avidity for the immunodominant, H-2D(d) restricted, P18-I10 epitope expand rapidly following immunization, and this expansion in turn determines the level of the P18-I10 epitope immunodominance. This proliferation has little dependence on the number of MHC-peptide complexes. Since most self-reactive T cells of high avidity are depleted in the thymus, the selection of immunodominant epitopes based on the expansion of high-avidity T cells in the periphery reduces the potential for autoimmunity. C1 NCI, Vaccine Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. RP Belyakov, IM (reprint author), NCI, Vaccine Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. EM igorbelyakov@yahoo.com; berzofsk@helix.nih.gov RI Margulies, David/H-7089-2013; OI Margulies, David/0000-0001-8530-7375 FU Intramural NIH HHS NR 54 TC 30 Z9 30 U1 0 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0953-8178 J9 INT IMMUNOL JI Int. Immunol. PD APR PY 2007 VL 19 IS 4 BP 497 EP 507 DI 10.1093/intimm/dxm016 PG 11 WC Immunology SC Immunology GA 152HP UT WOS:000245352800015 PM 17376783 ER PT J AU Andersson, J Stefanova, I Stephens, GL Shevach, EM AF Andersson, John Stefanova, Irena Stephens, Geoffrey L. Shevach, Ethan M. TI CD4(+)CD25(+) regulatory T cells are activated in vivo by recognition of self SO INTERNATIONAL IMMUNOLOGY LA English DT Article DE IL-2; IL-4; regulatory T cells; TCR; tolerance ID DENDRITIC CELLS; SUPPRESSOR FUNCTION; CUTTING EDGE; CD25(+); RECEPTOR; IL-2; SURVIVAL; FOXP3; STATE; INTERLEUKIN-2 AB Naturally occurring CD4(+)CD25(+) regulatory T cells (nT(R)) comprise a separate lineage of T cells that are essential for maintaining immunological tolerance to self. Here we demonstrate that the level of phosphorylation of the TCR zeta-chain is similar to 1.5- to 4-fold higher in nT(R) as compared with CD4(+)CD25(-) T cells. The increased level of TCR zeta-chain phosphorylation is presumably secondary to their higher affinity for self, resulting in a stronger TCR signal as it was completely blocked by treatment with anti-MHC class II. The enhanced level of TCR zeta-chain phosphorylation was correlated with the capacity of nT(R) to develop non-specific suppressor effector function following culture with IL-2 or IL-4 in the absence of TCR stimulus. Thus, a sub-population of nT(R) is activated by recognition of self-peptide-MHC class II ligands in vivo, resulting in their capacity to be induced to mediate suppressor function in vitro in the absence of TCR stimulation. C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. RP Shevach, EM (reprint author), NIAID, Immunol Lab, NIH, 10 Ctr Dr,MSC 1892, Bethesda, MD 20892 USA. EM eshevach@niaid.nih.gov RI Andersson, John/A-4436-2009; OI Andersson, John/0000-0003-2799-6349 NR 36 TC 24 Z9 24 U1 0 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0953-8178 J9 INT IMMUNOL JI Int. Immunol. PD APR PY 2007 VL 19 IS 4 BP 557 EP 566 DI 10.1093/intimm/dxm021 PG 10 WC Immunology SC Immunology GA 152HP UT WOS:000245352800020 PM 17369190 ER PT J AU Kong, Y Ruan, LF Ma, LL Cui, YH Wang, JM Le, YY AF Kong, Yan Ruan, Lingfei Ma, Lili Cui, Youhong Wang, Ji Ming Le, Yingying TI RNA interference as a novel and powerful tool in immunopharmacological research SO INTERNATIONAL IMMUNOPHARMACOLOGY LA English DT Review DE RNA interference; siRNA; therapeutics; immune system ID DOUBLE-STRANDED-RNA; FORMYLPEPTIDE RECEPTOR FPR; FORMYL-PEPTIDE RECEPTORS; NF-KAPPA-B; IN-VIVO; GENE-EXPRESSION; MAMMALIAN-CELLS; DENDRITIC CELLS; BREAST-CANCER; SIRNA DESIGN AB RNA interference (RNAi), as an evolutionarily conserved mechanism for silencing gene expression, is realized through the actions of both small interference RNA (siRNA) and microRNA. Since its discovery, siRNA has been rapidly deployed not only for the elucidation of gene function, but also for identification of drug targets and as a powerful therapeutic approach for a variety of diseases. In this review, we briefly introduce the mechanisms of RNAi, methods of siRNA design and delivery, and summarized recent researches on the therapeutic potential of RNAi for immune diseases. (c) 2007 Elsevier B.V. All rights reserved. C1 Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Nutr Sci,Lab Immunol & Inflammatory Dis, Shanghai, Peoples R China. NCI, Canc Res Ctr, Mol Immunoregulat Lab, Frederick, MD 21701 USA. RP Le, YY (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Nutr Sci,Lab Immunol & Inflammatory Dis, Shanghai, Peoples R China. EM yyle@sibs.ac.cn NR 92 TC 8 Z9 10 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1567-5769 J9 INT IMMUNOPHARMACOL JI Int. Immunopharmacol. PD APR PY 2007 VL 7 IS 4 BP 417 EP 426 DI 10.1016/j.intimp.2006.12.011 PG 10 WC Immunology; Pharmacology & Pharmacy SC Immunology; Pharmacology & Pharmacy GA 149KI UT WOS:000245145400002 PM 17321464 ER PT J AU Staines, HM Alkhalil, A Allen, RJ De Jonge, HR Derbyshire, E Egee, S Ginsburg, H Hill, DA Huber, SM Kirk, K Lang, F Lisk, G Oteng, E Pillai, AD Rayavara, K Rouhani, S Saliba, KJ Shen, C Solomon, T Thomas, SLY Verloo, P Desai, SA AF Staines, Henry M. Alkhalil, Abdulnaser Allen, Richard J. De Jonge, Hugo R. Derbyshire, Elvira Egee, Stephane Ginsburg, Hagai Hill, David A. Huber, Stephan M. Kirk, Kiaran Lang, Florian Lisk, Godfrey Oteng, Eugene Pillai, Ajay D. Rayavara, Kempaiah Rouhani, Sherin Saliba, Kevin J. Shen, Crystal Solomon, Tsione Thomas, Serge L. Y. Verloo, Patrick Desai, Sanjay A. TI Electrophysiological studies of malaria parasite-infected erythrocytes: Current status SO INTERNATIONAL JOURNAL FOR PARASITOLOGY LA English DT Article DE patch-clamp; ion channels; new permeability pathways; PSAC; plasmodium; oxidation ID RED-BLOOD-CELLS; CYSTIC-FIBROSIS PATIENTS; PLASMODIUM-FALCIPARUM; PERMEABILITY PATHWAYS; ANION CHANNEL; MEMBRANE; TRANSPORT AB The altered permeability characteristics of erythrocytes infected with malaria parasites have been a source of interest for over 30 years. Recent electrophysiological studies have provided strong evidence that these changes reflect transmembrane transport through ion channels in the host erythrocyte plasma membrane. However, conflicting results and differing interpretations of the data have led to confusion in this field. In an effort to unravel these issues, the groups involved recently came together for a week of discussion and experimentation. In this article, the various models for altered transport are reviewed, together with the areas of consensus in the field and those that require a better understanding. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. C1 St Georges Univ London, Div Cellular & Mol Med, Ctr Infect, London SW17 0RE, England. Natl Inst Allergy Infect Dis, Lab Malaria & Vector Res, NIH, Bethesda, MD USA. Australian Natl Univ, Sch Biochem & Mol Biol, Canberra, ACT 0200, Australia. Erasmus Univ, Ctr Med, Dept Biochem, NL-3000 DR Rotterdam, Netherlands. CNRS, Stn Biol, UMR 7127, F-29682 Roscoff, France. Hebrew Univ Jerusalem, Inst Life Sci, Dept Biol Chem, IL-91904 Jerusalem, Israel. Univ Tubingen, Dept Physiol, D-72076 Tubingen, Germany. Australian Natl Univ, Sch Med, Canberra, ACT 0200, Australia. RP Staines, HM (reprint author), St Georges Univ London, Div Cellular & Mol Med, Ctr Infect, London SW17 0RE, England. EM hstaines@sgul.ac.uk RI Allen, Richard/B-4752-2008; Staines, Henry/B-6281-2009; Desai, Sanjay/B-7110-2009; Saliba, Kevin/C-9166-2009; Kirk, Kiaran/C-8299-2009; OI Staines, Henry/0000-0001-5890-3040; Kirk, Kiaran/0000-0002-5613-2622; Hill, David/0000-0001-9286-4268 FU Intramural NIH HHS [Z01 AI000882-07]; Wellcome Trust [076441] NR 14 TC 66 Z9 67 U1 0 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0020-7519 J9 INT J PARASITOL JI Int. J. Parasit. PD APR PY 2007 VL 37 IS 5 BP 475 EP 482 DI 10.1016/j.ijpara.2006.12.013 PG 8 WC Parasitology SC Parasitology GA 158ET UT WOS:000245774800003 PM 17292372 ER PT J AU Schlee, M Holzel, M Bernard, S Mailhammer, R Schuhmacher, M Reschke, J Eick, D Marinkovic, D Wirth, T Rosenwald, A Staudt, LM Eilers, M Baran-Marszak, F Fagard, R Feuillard, J Laux, G Bornkamm, GW AF Schlee, Martin Hoelzel, Michael Bernard, Sandra Mailhammer, Reinhard Schuhmacher, Marino Reschke, Judith Eick, Dirk Marinkovic, Dragan Wirth, Thomas Rosenwald, Andreas Staudt, Louis M. Eilers, Martin Baran-Marszak, Fanny Fagard, Remi Feuillard, Jean Laux, Gerhard Bornkamm, Georg W. TI c-MYC activation impairs the NF-kappa B and the interferon response: Implications for the pathogenesis of Burkitt's lymphoma SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article DE Burkitt's lymphoma; c-myc; STAT1; interferon; NF-kappa B; antigen presentation ID TRANSCRIPTION FACTOR IRF-1; VIRUS NUCLEAR ANTIGEN-1; T-CELL EPITOPES; LATENT MEMBRANE-PROTEIN-1; TARGET GENES; LYMPHOPROLIFERATIVE DISEASE; ANTIVIRAL DEFENSE; REGULATES STAT1; DNA-DAMAGE; IFN-GAMMA AB Deregulation of the proto-oncogene c-myc is a key event in the pathogenesis of many tumors. A paradigm is the activation of the c-myc gene by chromosomal translocations in Burkitt lymphoma (BL). Despite expression of a restricted set of Epstein-Barr viral (EBV) antigens, BL cells are not recognized by antigen-specific cytotoxic T cells (CTLs) because of their inability to process and present HLA class I-restricted antigens. In contrast, cells of EBV-driven posttransplant lymphoproliferative disease (PTLD) are recognized and rejected by EBV-specific CTLs. It is not known whether the poor immunogenicity of BL cells is due to nonexpression of viral antigens, overexpression of c-myc, or both. To understand the basis for immune recognition and escape, we have compared the mRNA expression profiles of BL and EBV-immortalized cells (as PTLD model). Among the genes expressed at low level in BL cells, we have identified many genes involved in the NF-kappa B and interferon response that play a pivotal role in antigen presentation and immune recognition. Using a cell line in which EBNA2 and c-myc can be regulated at will, we show that c-MYC negatively regulates STAT1, the central player linking the Type-I and Type-H interferon response. Switching off c-myc expression leads to STAT1 induction through a direct and indirect mechanism involving induction of Type-I interferons. c-MYC thus masks an interferon-inducing activity in these cells. Our findings imply that immune escape of tumor cells is not only a matter of in vivo selection but may be additionally promoted by activation of a cellular oncogene. (c) 2007 Wiley-Liss. Inc. C1 GSF, Inst Clin Mol Biol & Tumor Genet, Natl Res Ctr Environm & Hlth, D-81377 Munich, Germany. Univ Marburg, Inst Mol Biol & Tumor Res, Marburg, Germany. GPC Biotech AG, Martinsried, Germany. Univ Ulm, Dept Physiol Chem, Ulm, Germany. NCI, Lymphoid Malignancies Sect, Metab Branch, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. Univ Paris 13, UPRES EA 3406, Bobigny, France. Univ Limoges, CNRS, UMR 6101, Fac Med, Limoges, France. CHU Dupuytren, Hematol Lab, Limoges, France. RP Bornkamm, GW (reprint author), GSF, Inst Clin Mol Biol & Tumor Genet, Natl Res Ctr Environm & Hlth, Marchioninistr 25, D-81377 Munich, Germany. EM bornkamm@gsf.de OI Eilers, Martin/0000-0002-0376-6533 FU Intramural NIH HHS NR 57 TC 34 Z9 35 U1 0 U2 2 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD APR 1 PY 2007 VL 120 IS 7 BP 1387 EP 1395 DI 10.1002/ijc.22372 PG 9 WC Oncology SC Oncology GA 141WW UT WOS:000244610700002 PM 17211884 ER PT J AU Sutcliffe, S Giovannucci, E Leitzmann, MF Rimm, EB Stampfer, MJ Willett, WC Platz, EA AF Sutcliffe, Siobhan Giovannucci, Edward Leitzmann, Michael F. Rimm, Eric B. Stampfer, Meir J. Willett, Walter C. Platz, Elizabeth A. TI A prospective cohort study of red wine consumption and risk of prostate cancer SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article DE red wine; alcoholic beverages; prostate cancer; cohort study ID ALCOHOL-CONSUMPTION; HEALTH-PROFESSIONALS; PHYSICAL-ACTIVITY AB In light of recent, strong inverse findings between lifetime red wine consumption and prostate cancer among younger men, we revisited our previous cohort analysis to more thoroughly investigate red wine consumption and prostate cancer in the Health Professionals Follow-up Study (HPFS). In 1986, HPFS participants reported their average consumption of red wine, white wine, beer and liquor during the past year, and their change in alcohol consumption over the prior 10 years. Prostate cancer diagnoses were ascertained on each biennial questionnaire and confirmed by medical record review. Between 1986 and 2002, 3,348 cases of prostate cancer were diagnosed among 45,433 eligible participants. Using men who did not consume red wine as the reference, no linear trend was observed between red wine consumption and prostate cancer in the full analytic cohort (p-trend = 0.57). Among men with unchanged alcohol consumption in the prior 10 years, and those additionally < 65 years of age, slightly lower risks were observed for men who consumed <= 4 glasses of red wine/week, whereas null or slight increased risks were observed for men who consumed > 4 glasses/week, resulting in a lack of linear trend. These findings suggest that red wine does not contribute appreciably to the etiology of prostate cancer. (c) 2007 Wiley-Liss, Inc. C1 Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD 21205 USA. Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. Harvard Univ, Sch Med, Channing Lab, Dept Med, Boston, MA 02115 USA. Brigham & Womens Hosp, Boston, MA 02115 USA. NCI, Div Canc Epidemiol & Genet, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. James Buchanan Brady Urol Inst, Baltimore, MD USA. Johns Hopkins Med Inst, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21205 USA. RP Platz, EA (reprint author), Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Epidemiol, Room E6138,615 N Wolfe St, Baltimore, MD 21205 USA. EM eplatz@jhsph.edu OI Sutcliffe, Siobhan/0000-0002-4613-8107 FU NCI NIH HHS [P50CA58236, CA55075]; NIAAA NIH HHS [AA11181]; NIDDK NIH HHS [P30 DK040561, P30 DK040561-11] NR 16 TC 23 Z9 23 U1 0 U2 2 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD APR 1 PY 2007 VL 120 IS 7 BP 1529 EP 1535 DI 10.1002/ijc.22498 PG 7 WC Oncology SC Oncology GA 141WW UT WOS:000244610700021 PM 17211860 ER PT J AU Tanofsky-Kraff, M Theim, KR Yanovski, SZ Bassett, AM Burns, NP Ranzenhofer, LM Glasofer, DR Yanovski, JA AF Tanofsky-Kraff, Marian Theim, Kelly R. Yanovski, Susan Z. Bassett, Allison M. Burns, Noel P. Ranzenhofer, Lisa M. Glasofer, Deborah R. Yanovski, Jack A. TI Validation of the Emotional Eating Scale adapted for use in children and adolescents (EES-C) SO INTERNATIONAL JOURNAL OF EATING DISORDERS LA English DT Article; Proceedings Paper CT Annual Meeting of the Pediatric-Academic-Societies/Society-of-Pediatric-Research CY APR 29-MAY 02, 2006 CL San Francisco, CA SP Pediat Acad Soc, Soc Pediat Res DE emotional eating; binge eating; loss of control eating; children and adolescents; overweight ID DISORDERED BEHAVIORS; OVERWEIGHT CHILDREN; GENDER DIFFERENCES; PUBERTAL CHANGES; WEIGHT PATTERNS; OBESE CHILDREN; BINGE; PSYCHOPATHOLOGY; DEPRESSION; QUESTIONNAIRE AB Objective: Eating in response to negative emotions is associated with binge or loss of control (LOC) eating in adults. Although children report engaging in LOC eating, data on emotional eating among youth are limited. Method: We adapted the adult Emotional Eating Scale (Arnow et al., Int J Eat Disord, 18, 79-90, 1995) to be used with children and adolescents (EES-C). Fifty-nine overweight (BMI >= 95th percentile for age and sex) and 100 non-overweight (BMI 5th-94th percentile) participants (mean age +/- SD 14.3 +/- 2.4 years) completed the EES-C, and measures of recent LOC eating and general psychopathology. Test-retest reliability was assessed in 64 children over a 3.4 +/- 2.6 month interval. Results: A factor analysis generated three subscales: eating in response to anxiety, anger, and frustration (EES-C-AAF), depressive symptoms (EES-C-DEP), and feeling unsettled (EES-C-UNS). Internal consistency for the subscales was established; Cronbach's alphas for the EES-C-AAF, EES-C-DEP, and EES-C-UNS were 0.95, 0.92, and 0.83, respectively. The EES-C had good convergent validity: children reporting recent LOC eating episodes scored higher on all subscales (p's < 0.05). The EES-C-AAF and EES-CUNS subscales demonstrated good discriminant validity and the EES-C-DEP revealed adequate discriminant validity. Intra-class correlation coefficients revealed good temporal stability for each subscale (EES-C-AAF = 0.59, EES-C-DEP = 0.74, EES-C-UNS = 0.66; p's < 0.001). Conclusion: The EES-C has good convergent and discriminant validity, and test-retest reliability for assessing emotional eating in children. Further investigation is required to clarify the role emotional eating may play in children's energy intake and body weight. (c) 2007 by Wiley Periodicals, Inc. C1 Uniformed Serv Univ Hlth Sci, Dept Med & Clin Psychol, Bethesda, MD 20814 USA. NICHD, Unit Growth & Obes, Dev Endocrinol Branch, NIH,DHHS, Bethesda, MD USA. NIDDK, Div Digest Dis & Nutr, NIH, DHHS, Bethesda, MD USA. RP Tanofsky-Kraff, M (reprint author), Uniformed Serv Univ Hlth Sci, Dept Med & Clin Psychol, 4301 Jones Bridge Rd, Bethesda, MD 20814 USA. EM mtanofsky@usuhs.mil RI Vollrath, Margarete/G-1297-2011; OI Yanovski, Jack/0000-0001-8542-1637 FU Intramural NIH HHS [Z01 HD000641-12, Z99 HD999999]; NICHD NIH HHS [Z01 HD000641, ZO1-HD-00641] NR 48 TC 63 Z9 65 U1 1 U2 15 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0276-3478 J9 INT J EAT DISORDER JI Int. J. Eating Disord. PD APR PY 2007 VL 40 IS 3 BP 232 EP 240 DI 10.1002/eat.20362 PG 9 WC Psychology, Clinical; Nutrition & Dietetics; Psychiatry; Psychology SC Psychology; Nutrition & Dietetics; Psychiatry GA 148QG UT WOS:000245089100006 PM 17262813 ER PT J AU Howarth, NC Huang, TTK Roberts, SB Lin, BH McCrory, MA AF Howarth, N. C. Huang, T. T-K Roberts, S. B. Lin, B-H McCrory, M. A. TI Eating patterns and dietary composition in relation to BMI in younger and older adults SO INTERNATIONAL JOURNAL OF OBESITY LA English DT Article DE energy intake; eating frequency; meal skipping; snacking; dietary fiber; dietary misreporting ID BODY-MASS INDEX; TOTAL-ENERGY INTAKE; 4 AGE-GROUPS; FOOD-INTAKE; HEALTHY-YOUNG; WEIGHT STATUS; US ADULTS; WOMEN; FREQUENCY; MEN AB Objective: To compare relative associations of eating patterns and dietary composition with body mass index (BMI) in younger (aged 20-59 years, n= 1792) and older (aged 60-90 years, n = 893) participants in the Continuing Survey of Food Intakes by Individuals, collected 1994-1996. Methods: Data from two 24-h dietary recalls from individuals reporting physiologically plausible energy intake (within +/- 22% of predicted energy requirements, based on previously published methods) were used. Results: Mean reported energy intake was 96 and 95% of predicted energy requirements in younger and older subjects, respectively. Older subjects were less likely than younger subjects to skip a meal, but snacking was common in both age groups. Fiber density was significantly higher in the older group. A higher BMI in both age groups was associated with a higher total daily energy intake, and higher energy intakes at all eating occasions. In both age groups, eating frequency was positively associated with energy intake, and eating more than three times a day was associated with being overweight or obese. In the younger group but not the older group, a lower fiber density coupled with higher percentage of energy from fat was independently associated with having a higher BMI. Conclusions: While no one eating occasion contributes more than any other to excess adiposity, eating more often than three times a day may play a role in overweight and obesity in both younger and older persons. A reduced satiety response to dietary fiber in addition to lower energy expenditure may potentially further contribute to weight gain in older persons. C1 Bastyr Univ, Sch Nutr & Exercise Sci, Kenmore, WA 98028 USA. Univ Hawaii, Canc Res Ctr Hawaii, Honolulu, HI 96813 USA. NICHHD, Bethesda, MD 20892 USA. Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr Aging, Energy Metab Lab, Boston, MA 02111 USA. Econ Res Serv, USDA, Washington, DC USA. RP McCrory, MA (reprint author), Bastyr Univ, Sch Nutr & Exercise Sci, 14500 Juanita Dr NE, Kenmore, WA 98028 USA. EM mmccrory@bastyr.edu NR 71 TC 94 Z9 95 U1 2 U2 28 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0307-0565 J9 INT J OBESITY JI Int. J. Obes. PD APR PY 2007 VL 31 IS 4 BP 675 EP 684 DI 10.1038/sj.ijo.0803456 PG 10 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA 154JK UT WOS:000245502800015 PM 16953255 ER PT J AU Huff, J Lunn, RM Waalkes, MP Tomatis, L Infante, PF AF Huff, James Lunn, Ruth M. Waalkes, Michael P. Tomatis, Lorenzo Infante, Peter F. TI Cadmium-induced cancers in animals and in humans SO INTERNATIONAL JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH LA English DT Review DE cadmium; carcinogenicity; animal studies; environmental exposures; epidemiologic studies; public health; occupational exposures; cancer bioassays; extrapolation ID INDUCED MALIGNANT-TRANSFORMATION; INTERSTITIAL CELL TUMORS; NOBLE NBL/CR RAT; PROSTATE-CANCER; PANCREATIC-CANCER; LUNG-CANCER; CARCINOGENESIS BIOASSAYS; PROLIFERATIVE LESIONS; RAMAZZINI-FOUNDATION; PRIMARY PREVENTION AB Discovered in the early 1800s, the use of cadmium and various cadmium salts started to become industrially important near the close of the 19th century, rapidly thereafter began to flourish, yet has diminished more recently. Most cadmium used in the United States is a byproduct from the smelting of zinc, lead, or copper ores, and is used to manufacture batteries. Carcinogenic activity of cadmium was discovered first in animals and only subsequently in humans. Cadmium and cadmium compounds have been classified as known human carcinogens by the International Agency for Research on Cancer and the National Toxicology Program based on epidemiologic studies showing a causal association with lung cancer, and possibly prostate cancer, and studies in experimental animals, demonstrating that cadmium causes tumors at multiple tissue sites, by various routes of exposure, and in several species and strains. Epidemiologic studies published since these evaluations suggest that cadmium is also associated with cancers of the breast, kidney, pancreas, and urinary bladder. The basic metal cationic portion of cadmium is responsible for both toxic and carcinogenic activity, and the mechanism of carcinogenicity appears to be multifactorial. Available information about the carcinogenicity. of cadmium and cadmium compounds is reviewed, evaluated, and discussed. C1 Natl Inst Environm Hlth Sci, Res Triangle Pk, NC 27514 USA. NCI, Bethesda, MD 20892 USA. Int Agcy Res Canc, F-69372 Lyon, France. Occupat Safety & Hlth Adm, Washington, DC USA. RP Huff, J (reprint author), Natl Inst Environm Hlth Sci, Res Triangle Pk, NC 27514 USA. EM huff1@niehs.nih.gov FU Intramural NIH HHS [Z99 ES999999] NR 120 TC 137 Z9 143 U1 4 U2 26 PU ABEL PUBLICATION SERVICES PI BURLINGTON PA 1611 AQUINAS COURT, BURLINGTON, NC 27215 USA SN 1077-3525 J9 INT J OCCUP ENV HEAL JI Int. J. Occup. Environ. Health PD APR-JUN PY 2007 VL 13 IS 2 BP 202 EP 212 PG 11 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 182GV UT WOS:000247494000009 PM 17718178 ER PT J AU Huff, J AF Huff, James TI Benzene-induced cancers: Abridged history and occupational health impact SO INTERNATIONAL JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH LA English DT Review DE benzene; carcinogenicity; industry influence; history; bioassay; public health; occupational safety; primary prevention ID NATIONAL-TOXICOLOGY-PROGRAM; TERM CHEMICAL CARCINOGENESIS; EXPERIMENTAL MULTIPOTENTIAL CARCINOGEN; INDUCED HEPATOCYTE PROLIFERATION; MAJOR RISK FACTOR; MALE F344 RATS; CELL-PROLIFERATION; PUBLIC-HEALTH; PRIMARY PREVENTION; B6C3F1 MICE AB Benzene-induced cancer in humans was first reported in the late 1920s. Carcinogenesis findings in animals were not reported conclusively until 1979. Industry exploited this "discrepancy" to discredit the use of animal bioassays as surrogates for human exposure experience. The cardinal reason for the delay between first recognizing leukemia in humans and sought-after neoplasia in animals centers on poor design and conduct of experimental studies. The first evidence of carcinogenicity in animals manifested as malignant tumors of the zymbal glands (sebaceous glands in the ear canal) of rats, and industry attempted to discount this as being irrelevant to humans, as this organ is vestigial and not present per se in humans. Nonetheless, shortly thereafter benzene was shown to be carcinogenic to multiple organ sites in both sexes of multiple strains and multiple species of laboratory animals exposed via various routes. This paper presents a condensed history of the benzene bioassay story with mention of benzene-associated human cancers. C1 Natl Inst Environm Hlth Sci, Res Triangle Pk, NC 27514 USA. RP Huff, J (reprint author), Natl Inst Environm Hlth Sci, Res Triangle Pk, NC 27514 USA. EM huff1@niehs.nih.gov FU Intramural NIH HHS [Z99 ES999999] NR 176 TC 40 Z9 41 U1 1 U2 6 PU ABEL PUBLICATION SERVICES PI BURLINGTON PA 1611 AQUINAS COURT, BURLINGTON, NC 27215 USA SN 1077-3525 J9 INT J OCCUP ENV HEAL JI Int. J. Occup. Environ. Health PD APR-JUN PY 2007 VL 13 IS 2 BP 213 EP 221 PG 9 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 182GV UT WOS:000247494000010 PM 17718179 ER PT J AU Matsumoto, KI Szajek, L Krishna, MC Cook, JA Seidel, J Grimes, K Carson, J Sowers, AL English, S Green, MV Bacharach, SL Eckelman, WC Mitchell, JB AF Matsumoto, Ken-Ichiro Szajek, Lawrence Krishna, Murali C. Cook, John A. Seidel, Jurgen Grimes, Kelly Carson, Joann Sowers, Anastasia L. English, Sean Green, Michael V. Bacharach, Stephen L. Eckelman, William C. Mitchell, James B. TI The influence of tumor oxygenation on hypoxia imaging in murine squamous cell carcinoma using [Cu-64]Cu-ATSM or [F-18]Fluoromisonidazole positron emission tomography SO INTERNATIONAL JOURNAL OF ONCOLOGY LA English DT Article DE tumor hypoxia; PET; imaging; Cu-ATSM; FMiso ID TISSUE OXYGENATION; RADIATION RESPONSE; LUNG-CANCER; IN-VIVO; PET; AGENT; RADIOTHERAPY; CU-64-ATSM; HEAD; NECK AB [Cu-64]Cu(II)-ATSM (Cu-64-ATSM) and [F-18]-Fluoromisonidazole (F-18-FMiso) tumor binding as assessed by positron emisson topography (PET) was used to determine the responsiveness of each probe to modulation in tumor oxygenation levels in the SCCVII tumor model. Animals bearing the SCCVII tumor were injected with 64Cu-ATSM or F-18-FMiso followed by dynamic small animal PET imaging. Animals were imaged with both agents using different inspired oxygen mixtures (air, 10% oxygen, carbogen) which modulated tumor hypoxia as independently assessed by the hypoxia marker pimonidazole. The extent of hypoxia in the SCCVII tumor as monitored by the pimonidazole hypoxia marker was found to be in the following order: 10% oxygen > air > carbogen. Tumor uptake of Cu-64-ATSM could not be changed if the tumor was oxygenated using carbogen inhalation 90 min post-injection suggesting irreversible cellular uptake of the Cu-64-ATSM complex. A small but significant paradoxical increase in Cu-64-ATSM tumor uptake was observed for animals breathing air or carbogen compared to 10% oxygen. There was a positive trend toward F-18-FMiso tumor uptake as a function of changing hypoxia levels in agreement with the pimonidazole data. Cu-64-ATSM tumor uptake was unable to predictably detect changes in varying amounts of hypoxia when oxygenation levels in SCCVII tumors were modulated. F-18-FMiso tumor uptake was more responsive to changing levels of hypoxia. While the mechanism of nitroimidazole binding to hypoxic cells has been extensively studied, the avid binding of Cu-ATSM to tumors may involve other C1 NCI, Radiat Biol Branch, Canc Res Ctr, NIH, Bethesda, MD 20892 USA. Natl Inst Hlth Clin Ctr, PET Dept, Bethesda, MD USA. Natl Inst Hlth Clin Ctr, Dept Nucl Med, Bethesda, MD USA. RP Mitchell, JB (reprint author), NCI, Radiat Biol Branch, Canc Res Ctr, NIH, Bldg 10,Room B3-B69,9000 Rockville Pike, Bethesda, MD 20892 USA. EM jbm@helix.nih.gov FU Intramural NIH HHS NR 32 TC 51 Z9 51 U1 0 U2 5 PU PROFESSOR D A SPANDIDOS PI ATHENS PA 1, S MERKOURI ST, EDITORIAL OFFICE,, ATHENS 116 35, GREECE SN 1019-6439 J9 INT J ONCOL JI Int. J. Oncol. PD APR PY 2007 VL 30 IS 4 BP 873 EP 881 PG 9 WC Oncology SC Oncology GA 147RP UT WOS:000245020400013 PM 17332926 ER PT J AU Begnami, MD Rushing, EJ Santi, M Quezado, M AF Begnami, Maria D. Rushing, Elisabeth J. Santi, Mariarita Quezado, Martha TI Evaluation of NF2 gene deletion in pediatric meningiomas using chromogenic in situ hybridization SO INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY LA English DT Article DE neurofibromatosis type II; NF2 gene; CISH; pediatric meningioma ID INTRACRANIAL MENINGIOMAS; SPORADIC MENINGIOMAS; MENINGEAL TUMORS; 2ND NEOPLASMS; BRAIN-TUMORS; NEUROFIBROMATOSIS; CHILDHOOD; RADIATION; CHILDREN; CHROMOSOME-22 AB Meningiomas are uncommon childhood tumors. They could be of significant size at presentation, which has been associated with difficult surgical excision, high recurrence rate, and possibly aggressive clinical behavior. Monosomy 22 is a common molecular event in this neoplasm. Additionally, losses on chromosomes 1,7,10, and 14 have been identified in clinically aggressive meningiomas. Using chromogenic in situ hybridization, we studied a group of pediatric meningiomas, including neurofibromatosis type II-associated, sporadic, and radiation-induced cases. We found NF2 gene deletion in about 72% of the cases, with corresponding absent or minimal merlin protein expression by immunohistochemistry. Our findings confirm that the NF2 gene plays a role in the tumorigenesis of pediatric meningiomas and that chromogenic in situ hybridization is an efficient, economic, and reliable method for routinely assessing NF2 gene deletion in formalin-fixed, paraffin-embedded tissues. C1 NCI, Dept Pathol, Surg Pathol Sect, NIH, Bethesda, MD 20892 USA. Armed Forces Inst Pathol, Dept Neuropathol & Ophthalm Pathol, Washington, DC 20306 USA. Childrens Hosp, Natl Med Ctr, Dept Pathol, Washington, DC 20010 USA. RP Quezado, M (reprint author), NCI, Dept Pathol, Surg Pathol Sect, NIH, Bldg 10,Room 2N214,10 Ctr Dr, Bethesda, MD 20892 USA. EM quezadom@mail.nih.gov RI Begnami, Maria/D-9663-2012 OI Begnami, Maria/0000-0003-0848-7813 NR 36 TC 7 Z9 7 U1 0 U2 0 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1066-8969 J9 INT J SURG PATHOL JI Int. J. Surg. Pathol. PD APR PY 2007 VL 15 IS 2 BP 110 EP 115 DI 10.1177/1066896906299128 PG 6 WC Pathology; Surgery SC Pathology; Surgery GA 148XK UT WOS:000245110500003 PM 17478763 ER PT J AU Ahmed, AA Tsokos, M AF Ahmed, Atif Ali Tsokos, Maria TI Sinonasal rhabdomyosarcoma in children and young adults SO INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY LA English DT Article DE nasal sinuses; rhabdomyosarcoma; immunohistochemistry ID CLEAR-CELL RHABDOMYOSARCOMA; NECK RHABDOMYOSARCOMA; HEAD; ADOLESCENTS; MYOGENIN; SARCOMAS; SINUSES; REGION AB Rhabdomyosarcoma is an aggressive malignant tumor often developing in the head and neck in children. In the sinonasal region, rhabdomyosarcoma constitutes a clinically important group because of the difficulty of surgical resection and its generally poor prognosis. We reviewed the archival pathology materials of 39 cases of rhabdomyosarcoma of the head and neck in children and young adults. The diagnosis was made through light microscopy, immunohistochemistry, electron microscopy, and/or reverse-transcriptase polymerase chain reaction (RT-PCR) molecular testing. We identified 14 tumors in the nose and paranasal sinuses. Patients' ages ranged from 9 to 40 years. Thirteen of the tumors were of the alveolar subtype. In 11 cases, the tumor cells were poorly differentiated, forming a solid alveolar pattern. In 2 cases, there was evidence of rhabdomyoblastic differentiation. Only one case was classified as embryonal rhabdomyosarcoma. A significant number of tumor cells in these cases had clear or vacuolated cytoplasm. Four alveolar rhabdomyosarcoma tumors were tested by RTPCR; all showed PAX3/FKHR chromosomal translocation. We conclude that sinonasal rhabdomyosarcoma is predominantly of the alveolar subtype and frequently shows clear cells. A review of the literature shows that these tumors carry a poor prognosis, partly because of their parameningeal location and partly because of their "undifferentiated" alveolar histology. C1 Childrens Natl Med Ctr, Div Anat Pathol, Washington, DC 20010 USA. NCI, Div Pediat Tumor Biol & Ultrastruct Pathol, NIH, Bethesda, MD 20892 USA. RP Ahmed, AA (reprint author), Childrens Natl Med Ctr, Div Anat Pathol, 111 Michigan Ave NW, Washington, DC 20010 USA. EM aahmed@cnmc.org OI Ahmed, Atif/0000-0002-8791-5785 NR 22 TC 10 Z9 12 U1 0 U2 0 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1066-8969 J9 INT J SURG PATHOL JI Int. J. Surg. Pathol. PD APR PY 2007 VL 15 IS 2 BP 160 EP 165 DI 10.1177/1066896906299122 PG 6 WC Pathology; Surgery SC Pathology; Surgery GA 148XK UT WOS:000245110500010 PM 17478770 ER PT J AU Sher, L Merrick, J AF Sher, Leo Merrick, Joav TI Alcohol and the brain SO INTERNATIONAL JOURNAL ON DISABILITY AND HUMAN DEVELOPMENT LA English DT Editorial Material C1 [Sher, Leo] Columbia Univ, Med Ctr, Div Neurosci, Dept Psychiat, New York, NY 10032 USA. [Merrick, Joav] Minist Social Affairs, Jerusalem, Israel. [Merrick, Joav] NICHHD, Bethesda, MD USA. RP Sher, L (reprint author), Columbia Univ, Med Ctr, Div Neurosci, Dept Psychiat, New York, NY 10032 USA. EM leosher@neuron.cpmc.columbia.edu; jrnerrick@zahav.net.il NR 13 TC 0 Z9 0 U1 0 U2 1 PU FREUND PUBLISHING HOUSE LTD PI TEL AVIV PA PO BOX 35010, TEL AVIV 61350, ISRAEL SN 1565-012X J9 INT J DISABIL HUM DE JI Int. J. Disabil. Hum. Dev. PD APR-JUN PY 2007 VL 6 IS 2 BP 113 EP 114 PG 2 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 279YJ UT WOS:000254391400001 ER PT J AU Zhang, SM Bodenreider, O AF Zhang, Songmao Bodenreider, Olivier TI Experience in aligning anatomical ontologies SO INTERNATIONAL JOURNAL ON SEMANTIC WEB AND INFORMATION SYSTEMS LA English DT Article DE anatomy; artificial intelligence; knowledge models; medical knowledge; ontologies; semantic matching ID BIOMEDICAL ONTOLOGIES; SEMANTIC-INTEGRATION; FOUNDATIONAL MODEL; BIOINFORMATICS; REPRESENTATION AB The objective of this article is to recapitulate our experience in aligning large anatomical ontologies (Foundational Model of Anatomy, GALEN, Adult Mouse Anatomical Dictionary and NCI Thesaurus) having different representation formalisms. Our approach to aligning concepts (directly) is automatic, rule-based, and operates at the schema level, generating mostly point-to-point mappings. It uses a combination of lexical, structural and semantic techniques. It also takes advantage of domain-specific knowledge (lexical knowledge from external resources, such ay the Unified Medical Language System, as well as knowledge augmentation and inference techniques). In addition to point-to-point mapping of concepts, we present the alignment of relationships and the mapping of concepts group-to-group. We have also successfully tested an indirect alignment through a reference ontology. We present an evaluation of our techniques, both against a gold standard and against a generic schema matching system. The advantages and limitations of our approach arc, analyzed and discussed throughout the article. C1 Chinese Acad Sci, Beijing 100864, Peoples R China. US Natl Lib Med, Bethesda, MD 20894 USA. RP Zhang, SM (reprint author), Chinese Acad Sci, Beijing 100864, Peoples R China. RI Smith, Barry/A-9525-2011 OI Smith, Barry/0000-0003-1384-116X FU Intramural NIH HHS [Z99 LM999999] NR 49 TC 43 Z9 43 U1 1 U2 4 PU IGI PUBL PI HERSHEY PA 701 E CHOCOLATE AVE, STE 200, HERSHEY, PA 17033-1240 USA SN 1552-6283 EI 1552-6291 J9 INT J SEMANT WEB INF JI Int. J. Semant. Web Inf. Syst. PD APR-JUN PY 2007 VL 3 IS 2 BP 1 EP 26 DI 10.4018/jswis.2007040101 PG 26 WC Computer Science, Artificial Intelligence; Computer Science, Information Systems SC Computer Science GA 214TH UT WOS:000249760500002 PM 18974854 ER PT J AU Burstein, HJ Overmoyer, B Gelman, R Silverman, P Savoie, J Clarke, K Dumadag, L Younger, J Ivy, P Winer, EP AF Burstein, Harold J. Overmoyer, Beth Gelman, Rebecca Silverman, Paula Savoie, Jennifer Clarke, Kathryn Dumadag, Leda Younger, Jerry Ivy, Percy Winer, Eric P. TI Rebeccamycin analog for refractory breast cancer: A randomized phase II trial of dosing schedules SO INVESTIGATIONAL NEW DRUGS LA English DT Article DE breast cancer; rebeccamycin; rebeccamycin analog; phase II ID NSC-655649 AB Rebeccamycin analog (NSC 655649) is a synthetic antibiotic cytotoxic agent thought to inhibit topoisomerase function. We sought to determine the response rate to rebeccamycin analog among patients with refractory advanced breast cancer using two different treatment schedules. Eligible patients had measurable disease, central venous access, and one or two prior chemotherapy regimens for advanced cancer, or recurrence within 12 months of adjuvant chemotherapy. Patients were randomized to rebeccamycin analog on one of two treatment schedules: arm 1, 500 mg/m2 IV bolus every 21 days; arm 2, 140 mg/m2 IV bolus daily x5 days, every 21 days. The primary study endpoint was response rate; a two stage accrual design evaluated each schedule separately. Forty-two women entered the trial, 21 on each arm. Prior chemotherapy regimens for metastatic breast cancer were: 0, n=4; 1, n=21; 2, n=17. Prior treatments (including adjuvant therapy) anthracyclines: 88%, taxanes 67%, 5FU-based therapy, 50%. There were 5 partial responses (overall response rate 12%), two in arm 1 and 3 in arm 2, all in patients with prior anthracycline-based adjuvant chemotherapy. Median time to progression was 2.1 months (range 1-14+ months). An additional 9 patients had stable disease as best response. Grade 3 or 4 toxicity rates were: anemia 5%, neutropenia 33%, thrombocytopenia 12%, RBC transfusion 14%, nausea/vomiting 10%. Toxicity profiles were similar between the treatment arms. Rebeccamycin analog is reasonably well tolerated on two different treatment schedules for advanced breast cancer, with modest clinical activity in this heavily pretreated population. C1 Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA. Univ Hosp Cleveland, Ireland Canc Ctr, Cleveland, OH 44106 USA. Massachusetts Gen Hosp, Boston, MA 02114 USA. NCI, Bethesda, MD 20892 USA. RP Burstein, HJ (reprint author), Harvard Univ, Sch Med, Dana Farber Canc Inst, 44 Binney St, Boston, MA 02115 USA. EM hburstein@partners.org NR 8 TC 10 Z9 10 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6997 J9 INVEST NEW DRUG JI Invest. New Drugs PD APR PY 2007 VL 25 IS 2 BP 161 EP 164 DI 10.1007/s10637-006-9007-6 PG 4 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA 119WW UT WOS:000243046100009 PM 16969707 ER PT J AU Gomez-Abuin, G Winquist, E Stadler, WM Pond, G Degendorfer, P Wright, J Moore, MJ AF Gomez-Abuin, Gonzalo Winquist, Eric Stadler, Walter M. Pond, Greg Degendorfer, Pamela Wright, John Moore, Malcolm J. TI A phase II study of PS-341 (Bortezomib) in advanced or metastatic urothelial cancer. A trial of the Princess Margaret Hospital and University of Chicago phase II consortia SO INVESTIGATIONAL NEW DRUGS LA English DT Article DE urothelial cancer; bortezomib ID PROTEASOME INHIBITOR PS-341; COOPERATIVE-ONCOLOGY-GROUP; REFRACTORY CARCINOMA; MULTIPLE-MYELOMA; MALIGNANCIES; GEMCITABINE; CISPLATIN; APOPTOSIS AB Background: Based on evidence of activity in epithelial tumors in preclinical and Phase I studies, its novel mechanism of action, and its tolerability we undertook a study of bortezomib [PS-341], a reversible proteasome inhibitor, for patients with advanced or metastatic urothelial cancer. Patients and methods: Patients with advanced or metastatic unresectable urothelial carcinoma were enrolled onto this multicenter, phase II trial. Patients with measurable disease were treated with bortezomib 1.3 mg/m2/day (twice weekly for 2 weeks out of 3) by intravenous infusion on days 1, 4, 8, and 11 every 21 days. A two stage phase II design was used. Results: Twenty-one patients were enrolled and twenty were eligible and received treatment. Eighty-five percent of patients had previous chemotherapy regimens. No objective responses were observed, median time-to-progression was 8.1 weeks (95% confidence interval [CI] 6.4 to 9), and median overall survival is estimated to be 15 weeks (95% CI 3.6 - NA). A total of 15 patients experienced a grade 3-4 adverse event. The most common were alkaline phosphatase (20% patients), lymphopenia (20% patients), myalgia (15% patients), dyspnea (15% patients) and thrombosis/embolism (15% patients). Conclusion: Single-agent bortezomib is an ineffective treatment for progressive-cisplatin-refractory urothelial carcinoma and should not be considered for future clinical trials in this population of patients. C1 Univ Chicago, Dept Med, Chicago, IL 60637 USA. NCI, Canc Therapy Evaluat Program, Bethesda, MD 20892 USA. RP Moore, MJ (reprint author), Univ Toronto, Princess Margaret Hosp, Rm 5-205,610 Univ Ave, Toronto, ON M5G 2M9, Canada. EM Malcolm.moore@uhn.on.ca FU NCI NIH HHS [N01 CM 17107, U01 CA69852-08] NR 18 TC 25 Z9 25 U1 0 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6997 J9 INVEST NEW DRUG JI Invest. New Drugs PD APR PY 2007 VL 25 IS 2 BP 181 EP 185 DI 10.1007/s10637-006-9009-4 PG 5 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA 119WW UT WOS:000243046100012 PM 16983508 ER PT J AU Iannaccone, A Mura, M Gallaher, KT Johnson, EJ Todd, WA Kenyon, E Harris, TL Harris, T Satterfield, S Johnson, KC Kritchevsky, SB AF Iannaccone, Alessandro Mura, Marco Gallaher, Kevin T. Johnson, Elizabeth J. Todd, William Andrew Kenyon, Emily Harris, Tarsha L. Harris, Tamara Satterfield, Suzanne Johnson, Karen C. Kritchevsky, Stephen B. CA Health ABC Study TI Macular pigment optical density in the elderly: Findings in a large biracial midsouth population sample SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Article; Proceedings Paper CT Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology CY APR 30-MAY 05, 2005 CL Ft Lauderdale, FL SP Assoc Res Vis & Ophthalmol ID AGE-RELATED MACULOPATHY; HETEROCHROMATIC FLICKER PHOTOMETRY; RESONANCE RAMAN MEASUREMENT; BLUE-MOUNTAINS-EYE; GEOGRAPHIC ATROPHY; LUTEIN SUPPLEMENTATION; VISUAL-ACUITY; ANTIOXIDANT STATUS; MOTION PHOTOMETRY; ALPHA-TOCOPHEROL AB PURPOSE. To report the macular pigment optical density (MPOD) findings at 0.5 degrees of eccentricity from the fovea in elderly subjects participating in ARMA, a study of aging and age-related maculopathy (ARM) ancillary to the Health, Aging, and Body Composition (Health ABC) Study. METHODS. MPOD was estimated with a heterochromatic flicker photometry (HFP) method in a large biracial population sample of normal 79.1 +/- 3.2-year-old adults living in the Midsouth (n = 222; 52% female; 23% black, 34% users of lutein-containing supplements). Within a modified testing protocol, subjects identified the lowest and the highest target intensity at which the flicker sensation disappeared, and the exact middle of this "no-flicker zone" was interpolated by the examiner. RESULTs. An MPOD estimate was obtained successfully in 82% of the participants. The mean MPOD in our sample was 0.34 +/- 0.21 (SD). The interocular correlation was high (Pearson's r = 0.82). Compared with lutein supplement users, mean MPOD was 21% lower in nonusers (P = 0.013). MPOD was also 41% lower in blacks than in whites (P = 0.0002), even after adjustment for lutein supplement use. There were no differences in MPOD by gender, iris color, or history of smoking. CONCLUSIONs. Older adults in the Midsouth appear to have average MPOD and interocular correlation comparable to those in previous studies. Lutein supplement use and white race correlated with higher MPOD. No evidence of an age-related decline in MPOD was seen in the sample. The HFP method for the measurement of MPOD is feasible in epidemiologic investigations of the elderly, the group at highest risk of ARM. C1 Univ Tennessee, Ctr Hlth Sci, Hamilton Eye Inst, Dept Ophthalmol, Memphis, TN 38163 USA. Univ Tennessee, Ctr Hlth Sci, Dept Prevent Med, Memphis, TN 38163 USA. Univ Cagliari, Dept Ophthalmol, Cagliari, Italy. Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA. NIA, NIH, Bethesda, MD 20892 USA. Wake Forest Univ, Sch Med, Stich Ctr Aging, Winston Salem, NC 27109 USA. RP Iannaccone, A (reprint author), Univ Tennessee, Ctr Hlth Sci, Hamilton Eye Inst, Dept Ophthalmol, 930 Madison Ave,Suite 731, Memphis, TN 38163 USA. EM iannacca@utmem.edu OI Mura, Marco/0000-0001-5283-5366 FU Intramural NIH HHS; NEI NIH HHS [K23 EY 000409, K23 EY000409-05, K23 EY000409]; NIA NIH HHS [N01 AG 62101, N01 AG062103, N01 AG062106, N01 AG 62106, N01 AG 62103, N01 AG062101]; NIDDK NIH HHS [T35 DK007405, T35 DK 07405] NR 65 TC 34 Z9 37 U1 0 U2 6 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD APR PY 2007 VL 48 IS 4 BP 1458 EP 1465 DI 10.1167/iovs.06-0438 PG 8 WC Ophthalmology SC Ophthalmology GA 153CB UT WOS:000245408200004 PM 17389471 ER PT J AU Zhang, F Au, MC Bouey, PD Zhao, Y Huang, ZHJ Dou, ZH Li, ZC Haberer, J Chen, RY Ya, L AF Zhang, Fujie Au, Maria C. Bouey, Paul D. Zhao, Yan Huang, Zhihuan Jennifer Dou, Zhihui Li, Zaicun Haberer, Jessica Chen, Ray Y. Ya, Lan TI The diagnosis and treatment of HIV-infected children in China - Challenges and opportunities SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Article DE China; diagnosis; highly active antiretroviral therapy; HIV; pediatrics ID HIGH PREVALENCE; AFRICA; HEALTH AB Background: HIV-infected children in China have not been well studied. This national survey describes the demographic characteristics and the associated diagnostic and antiretroviral treatment (ART) efforts directed toward surviving HIV-infected children. Methods: A cross-sectional study was conducted in the 6 provinces with the highest HIV prevalence: 4 former plasma donation (FPD) provinces and 2 intravenous drug use (IDU) provinces. A survey on demographics and treatment-related issues was distributed to the parents or guardians of all living HIV-infected children identified through the national case reporting system. Descriptive and bivariate analyses were performed on completed surveys. Results: Six hundred ninety-two (62.4%) of the total 1108 surveys were returned, and 650 were eligible for analysis. The average age in FPD provinces (mean +/- SD: 8.1 +/- 3.2 years) was significantly older than in IDU provinces (mean +/- SD: 5.4 +/- 2.2 years; P < 0.001). The average lag time from the probable date of transmission to a diagnosis for patients with mother-to-child transmission (MTCT) was 6.7 +/- 3.1 years in the FPD provinces and 4.7 +/- 1.9 years in the IDU provinces (P < 0.001). On the basis of the CD4 cell count or World Health Organization staging, 29.8% (144 of 484) of children from all 6 provinces who were not on ART needed it. Conclusions: This first national pediatric survey indicates that the age and time required for diagnosis were greater in HIV-infected children from FPD provinces compared with those from IDU provinces. In addition, this survey highlights the prolonged delay in the diagnosis and initiation of ART for children in China. Aggressive efforts to identify HIV-positive pregnant women, scale up prevention of MTCT activities, and expand early diagnosis and treatment are urgently needed. C1 Natl Ctr AIDS STD Control & Prevent, China Ctr Dis Control, Div Treatment & Care, Beijing 100050, Peoples R China. San Francisco CA Clinton Fdn HIV AIDS Initiat, Pangea Global AIDS Fdn, Beijing, Peoples R China. Georgetown Univ, Washington, DC 20057 USA. Clinton Fdn HIV AIDS Initiat, Beijing, Peoples R China. NIAID, NIH, Div AIDS, Bethesda, MD 20892 USA. RP Zhang, F (reprint author), Natl Ctr AIDS STD Control & Prevent, China Ctr Dis Control, Div Treatment & Care, 27 Nanwei Rd, Beijing 100050, Peoples R China. EM treatment@chinaaids.cn OI Chen, Ray/0000-0001-6344-1442 NR 19 TC 14 Z9 15 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR 1 PY 2007 VL 44 IS 4 BP 429 EP 434 DI 10.1097/QAI.0b013e31803133ac PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 146CW UT WOS:000244913100009 PM 17224849 ER PT J AU Reed, MB Gagneux, S DeRiemer, K Small, PM Barry, CE AF Reed, Michael B. Gagneux, Sebastien DeRiemer, Kathryn Small, Peter M. Barry, Clifton E., III TI The W-Beijing lineage of Mycobacterium tuberculosis overproduces triglycerides and has the DosR dormancy regulon constitutively upregulated SO JOURNAL OF BACTERIOLOGY LA English DT Article ID HYPOXIC RESPONSE; GLOBAL DISSEMINATION; GENOMIC DELETIONS; DRUG-RESISTANCE; IMMUNE-RESPONSE; CLONE FAMILY; BOVIS BCG; STRAINS; GENOTYPE; GENE AB The Beijing family of Mycobacterium tuberculosis strains has been associated with epidemic spread and an increased likelihood of developing drug resistance. The characteristics that predispose this family to such clinical outcomes have not been identified, although one potential candidate, the phenolic glycolipid PGL-tb, has been shown to mediate a fulminant lethal disease in mice and rabbits due to lipid-mediated immunosuppression. However, PGL-tb is not uniformly expressed throughout the Beijing lineage and may not be the only unique virulence trait associated with this family. In an attempt to define phenotypes common to all Beijing strains, we interrogated a carefully selected set of isolates representing the five extant lineages of the Beijing family. Comparison of lipid production in this set revealed that all Beijing strains accumulated large quantities of triacylglycerides in in vitro aerobic culture. This accumulation was found to be coincident with upregulation of Rv3130c, whose product was previously characterized as a triacyl glyceride synthase. Rv3130c is a member of the DosR-controlled regulon of M. tuberculosis, and further examination revealed that several members of this regulon were upregulated throughout this strain family. The upregulation of the DosR regulon may confer an adaptive advantage for growth in microaerophilic or anaerobic environments encountered by the bacillus during infection and thus may be related to the epidemiological phenomena associated with this important strain lineage. C1 NIH, TB Res Sect, Rockville, MD 20852 USA. Stanford Univ, Div Infect Dis & Geog Med, Stanford, CA 94305 USA. Inst Syst Biol, Seattle, WA 98103 USA. Univ Calif Davis, Sch Med, Davis, CA 95616 USA. Bill & Melinda Gates Fdn, Seattle, WA 98102 USA. RP Reed, MB (reprint author), McGill Univ, Res Inst, Ctr Hlth, Montreal Gen Hosp, 1650 Cedar Ave, Montreal, PQ H3G 1A4, Canada. EM michael.reed@mcgill.ca RI Barry, III, Clifton/H-3839-2012; OI DeRiemer, Kathryn/0000-0002-8375-7505 FU FIC NIH HHS [K01 TW000001, K01 TW000001-07]; Intramural NIH HHS [Z01 AI000783-11] NR 50 TC 135 Z9 144 U1 1 U2 10 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD APR PY 2007 VL 189 IS 7 BP 2583 EP 2589 DI 10.1128/JB.01670-06 PG 7 WC Microbiology SC Microbiology GA 159CC UT WOS:000245842000001 PM 17237171 ER PT J AU Bubunenko, M Baker, T Court, DL AF Bubunenko, Mikhail Baker, Teresa Court, Donald L. TI Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli SO JOURNAL OF BACTERIOLOGY LA English DT Article ID TERMINATION FACTOR-RHO; STATIONARY-PHASE; IN-VIVO; DEPENDENT TERMINATION; BACILLUS-SUBTILIS; MODULATION FACTOR; MESSENGER-RNA; NUS FACTORS; TRANSLATION; GROWTH AB We describe here details of the method we used to identify and distinguish essential from nonessential genes on the bacterial Escherichia coli chromosome. Three key features characterize our method: high-efficiency recombination, precise replacement of just the open reading frame of a chromosomal gene, and the presence of naturally occurring duplications within the bacterial genome. We targeted genes encoding functions critical for processes of transcription and translation. Proteins from three complexes were evaluated to determine if they were essential to the cell by deleting their individual genes. The transcription elongation Nus proteins and termination factor Rho, which are involved in rRNA antitermination, the ribosomal proteins of the small 30S ribosome subunit, and minor ribosome-associated proteins were analyzed. It was concluded that four of the five bacterial transcription antitermination proteins are essential, while all four of the minor ribosome-associated proteins examined (RMF, SRA, YfiA, and YhbH), unlike most ribosomal proteins, are dispensable. Interestingly, although most 30S ribosomal proteins were essential, the knockouts of six ribosomal protein genes, rpsF (S6), rpsI (S9), rpsM (S13), rpsO (S15), rpsQ (S17), and rpsT (S20), were viable. C1 NCI, Mol Control & Genet Sect, Gene Regulat & Chromosome Biol Lab, Ctr Canc Res, Frederick, MD 21702 USA. SAIC Frederick Inc, Natl Canc Inst, Basic Res Program, Frederick, MD 21702 USA. RP Court, DL (reprint author), NCI, Mol Control & Genet Sect, Gene Regulat & Chromosome Biol Lab, Ctr Canc Res, Frederick, MD 21702 USA. EM court@ncifcrf.gov FU Intramural NIH HHS; NCI NIH HHS [N01-CO-12400, N01CO12400] NR 72 TC 53 Z9 57 U1 2 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD APR PY 2007 VL 189 IS 7 BP 2844 EP 2853 DI 10.1128/JB.01713-06 PG 10 WC Microbiology SC Microbiology GA 159CC UT WOS:000245842000030 PM 17277072 ER PT J AU Mishra, A Das, A Cisar, JO Ton-That, H AF Mishra, Arunima Das, Asis Cisar, John O. Ton-That, Hung TI Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii SO JOURNAL OF BACTERIOLOGY LA English DT Article ID GRAM-POSITIVE BACTERIA; T14V TYPE-1 FIMBRIAE; CELL-WALL ENVELOPE; VISCOSUS T14V; CORYNEBACTERIUM-DIPHTHERIAE; SURFACE; CLONING; PILI; EXPRESSION; ANTIGENS AB Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy and biochemical analysis, we showed that the fimQ-fimP-srtC1-fimR gene cluster encodes a fimbrial structure (designated type 1) that contains a major subunit, FimP, forming the shaft and a minor subunit, FimQ, located primarily at the tip. Similarly, the fimB-fimA-srtC2 gene cluster encodes a distinct fimbrial structure (designated type 2) composed of a shaft protein, FimA, and a tip protein, FimB. By using allelic exchange, we constructed an in-frame deletion mutant that lacks the SrtC2 sortase. This mutant produces abundant type 1 fimbriae and expresses the monomeric FimA and FimB proteins, but it does not assemble type 2 fimbriae. Thus, SrtC2 is a fimbria-specific sortase that is essential for assembly of the type 2 fimbriae. Together, our experiments pave the way for several lines of molecular investigation that are necessary to elucidate the fimbrial assembly pathways in Actinomyces and their function in the pathogenesis of different biofilm-related oral diseases. C1 Univ Connecticut, Dept Mol Micorbial & Struct Biol, Ctr Hlth, Farmington, CT 06030 USA. NIDCR, Oral Infect & Immun Branch, NIH, Bethesda, MD 20892 USA. RP Ton-That, H (reprint author), Univ Connecticut, Dept Mol Micorbial & Struct Biol, Ctr Hlth, 263 Farmington Ave, Farmington, CT 06030 USA. EM ton-that@uchc.edu OI Ton-That, Hung/0000-0003-1611-0469 FU Intramural NIH HHS; NIAID NIH HHS [AI061381, R01 AI061381, R01 AI061381-02, R56 AI061381] NR 46 TC 61 Z9 66 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD APR PY 2007 VL 189 IS 8 BP 3156 EP 3165 DI 10.1128/JB.01952-06 PG 10 WC Microbiology SC Microbiology GA 161QB UT WOS:000246029300024 PM 17277070 ER PT J AU Iwanczyk, J Damjanovic, D Kooistra, J Leong, V Jomaa, A Ghirlando, R Ortega, J AF Iwanczyk, Jack Damjanovic, Daniela Kooistra, Joel Leong, Vivian Jomaa, Ahmad Ghirlando, Rodolfo Ortega, Joaquin TI Role of the PDZ domains in Escherichia coli DegP protein SO JOURNAL OF BACTERIOLOGY LA English DT Article ID EXCHANGER REGULATORY FACTOR; TAIL-SPECIFIC PROTEASE; CRYSTAL-STRUCTURE; SUBSTRATE RECOGNITION; TAGGING SYSTEM; PEPTIDE; DEGRADATION; CHAPERONE; HTRA; BINDING AB PDZ domains are modular protein interaction domains that are present in metazoans and bacteria. These domains possess unique structural features that allow them to interact with the C-terminal residues of their ligands. The Escherichia coli essential periplasmic protein DegP contains two PDZ domains attached to the C-terminal end of the protease domain. In this study we examined the role of each PDZ domain in the protease and chaperone activities of this protein. Specifically, DegP mutants with either one or both PDZ domains deleted were generated and tested to determine their protease and chaperone activities, as well as their abilities to sequester unfolded substrates. We found that the PDZ domains in DegP have different roles; the PDZ1 domain is essential for protease activity and is responsible for recognizing and sequestering unfolded substrates through C-terminal tags, whereas the PDZ2 domain is mostly involved in maintaining the hexameric cage of DegP. Interestingly, neither of the PDZ domains was required for the chaperone activity of DegP. In addition, we found that the loops connecting the protease domain to PDZ1 and connecting PDZ1 to PDZ2 are also essential for the protease activity of the hexameric DegP protein. New insights into the roles of the PDZ domains in the structure and function of DegP are provided. These results imply that DegP recognizes substrate molecules targeted for degradation and substrate molecules targeted for refolding in different manners and suggest that the substrate recognition mechanisms may play a role in the protease-chaperone switch, dictating whether the substrate is degraded or refolded. C1 McMaster Univ, Hlth Sci Ctr, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada. NIDDKD, Lab Mol Biol, NIH, DHHS, Bethesda, MD 20892 USA. RP Ortega, J (reprint author), McMaster Univ, Hlth Sci Ctr, Dept Biochem & Biomed Sci, Room 4H24,1200 Main St W, Hamilton, ON L8N 3Z5, Canada. EM ortegaj@mcmaster.ca RI Ghirlando, Rodolfo/A-8880-2009 FU Canadian Institutes of Health Research [76594, 82930]; Intramural NIH HHS NR 35 TC 45 Z9 45 U1 1 U2 7 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD APR PY 2007 VL 189 IS 8 BP 3176 EP 3186 DI 10.1128/JB.01788-06 PG 11 WC Microbiology SC Microbiology GA 161QB UT WOS:000246029300026 PM 17277057 ER PT J AU Stahl, AM Ruthel, G Torres-Melendez, E Kenny, TA Panchal, RG Bavari, S AF Stahl, Andrea M. Ruthel, Gordon Torres-Melendez, Edna Kenny, Tara A. Panchal, Rekha G. Bavari, Sina TI Primary cultures of embryonic chicken neurons for sensitive cell-based assay of botulinum neurotoxin: Implications for therapeutic discovery SO JOURNAL OF BIOMOLECULAR SCREENING LA English DT Article DE botulinum neurotoxin; chick neurons; SNAP-25 ID SMALL-MOLECULE INHIBITORS; TOXIN; IDENTIFICATION; TETANUS; PROTEIN AB Botulinum toxin is an exceedingly potent inhibitor of neurotransmission across the neuromuscular junction, causing flaccid paralysis and death. The potential for misuse of this deadly poison as a bioweapon has added a greater urgency to the search for effective therapeutics. The development of sensitive and efficient cell-based assays for the evaluation of toxin antagonists is crucial to the rapid and successful identification of therapeutic compounds. The authors evaluated the sensitivity of primary cultures from 4 distinct regions of the embryonic chick nervous system to botulinum neurotoxin A (BoNT/A) cleavage of synaptosomal-associated protein of 25 kD (SNAP-25). Although differences in sensitivity were apparent, SNAP-25 cleavage was detectable in neuronal cells from each of the 4 regions within 3 It at BoNT/A concentrations of 1 nM or lower. Co-incubation of chick neurons with BoNT/A and toxin-neutralizing antibodies inhibited SNAP-25 cleavage, demonstrating the utility of these cultures for the assay of BoNT/A antagonists. C1 USA, Med Res Inst Infect Dis, Frederick, MD 21702 USA. NCI, SAIC Frederick Inc, Target Struct Based Drug Discovery Grp, Frederick, MD 21701 USA. RP Ruthel, G (reprint author), USA, Med Res Inst Infect Dis, 1425 Porter St, Frederick, MD 21702 USA. EM gordon.ruthel@amedd.army.mil; sina.bavari@amedd.army.mil FU NCI NIH HHS [N01-CO-12400] NR 25 TC 24 Z9 24 U1 2 U2 4 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1087-0571 J9 J BIOMOL SCREEN JI J. Biomol. Screen PD APR PY 2007 VL 12 IS 3 BP 370 EP 377 DI 10.1177/1087057106299163 PG 8 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Chemistry GA 157WV UT WOS:000245753000008 PM 17332092 ER PT J AU Neckers, L AF Neckers, Len TI Heat shock protein 90: the cancer chaperone SO JOURNAL OF BIOSCIENCES LA English DT Article; Proceedings Paper CT International Symposium on Environmental Factors, Cellular Stress and Evolution CY OCT 13-15, 2006 CL Banaras Hindu Univ, Varanasi, INDIA HO Banaras Hindu Univ DE cancer; protein folding; Hsp90; huntingtin; molecular chaperone; neurodegeneration ID CHRONIC MYELOGENOUS LEUKEMIA; PROTEASOME INHIBITOR PS341; DT-DIAPHORASE EXPRESSION; CHRONIC MYELOID-LEUKEMIA; SHOCK-PROTEIN 90; TYROSINE KINASE; BCR-ABL; PARKINSONS-DISEASE; HSP90 INHIBITORS; MOLECULAR CHAPERONE AB Heat shock protein 90 (Hsp90) is a molecular chaperone required for the stability and function of a number of conditionally activated and/or expressed signalling proteins, as well as multiple mutated, chimeric, and/or over-expressed signalling proteins, that promote cancer cell growth and/or survival. Hsp90 inhibitors are unique in that, although they are directed towards a specific molecular target, they simultaneously inhibit multiple cellular signalling pathways. By inhibiting nodal points in multiple overlapping survival pathways utilized by cancer cells, combination of an Hsp90 inhibitor with standard chemotherapeutic agents may dramatically increase the in vivo efficacy of the standard agent. Hsp90 inhibitors may circumvent the characteristic genetic plasticity that has allowed cancer cells to eventually evade the toxic effects of most molecularly targeted agents. The mechanism-based use of Hsp90 inhibitors, both alone and in combination with other drugs, should be effective toward multiple forms of cancer. Further, because Hsp90 inhibitors also induce Hsf-1-dependent expression of Hsp70, and because certain mutated Hsp90 client proteins are neurotoxic, these drugs display ameliorative properties in several neurodegenerative disease models, suggesting a novel role for Hsp90 inhibitors in treating multiple pathologies involving neurodegeneration. C1 NCI, Urol Oncol Branch, Bethesda, MD 20892 USA. RP Neckers, L (reprint author), NCI, Urol Oncol Branch, Bethesda, MD 20892 USA. EM len@helix.nih.gov NR 122 TC 267 Z9 272 U1 3 U2 11 PU INDIAN ACADEMY SCIENCES PI BANGALORE PA C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA SN 0250-5991 J9 J BIOSCIENCES JI J. Biosci. PD APR PY 2007 VL 32 IS 3 BP 517 EP 530 DI 10.1007/s12038-007-0051-y PG 14 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 166KN UT WOS:000246377700011 PM 17536171 ER PT J AU Hu, JX Reyes-Cruz, G Goldsmith, PK Gantt, NM Miller, JL Spiegel, AM AF Hu, Jianxin Reyes-Cruz, Guadalupe Goldsmith, Paul K. Gantt, Nicole M. Miller, Jeffery L. Spiegel, Allen M. TI Functional effects of monoclonal antibodies to the purified amino-terminal extracellular domain of the human Ca2+ receptor SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Article DE human Ca2+ receptor; extracellular domain; Venus flytrap-like domain; monoclonal antibodies; chimeric receptor ID HUMAN CALCIUM RECEPTOR; CA2+-SENSING RECEPTOR; EXPRESSION AB Introduction: The human Ca2+ receptor (CaR), which plays a central role in the regulation of [Ca2+](0) homeostasis, has a distinctively large extracellular domain that consists of a bilobed Venus flytrap (VFT) domain, involved in agonist binding, and a cysteine-rich domain. Functional antibodies that specifically bind to this domain would have therapeutic potential and could be used as a tool to gain insights into receptor activation as well. Materials and Methods: We generated three monoclonal antibodies (mAbs), 7178, 5C8, and 1A8, to the purified human CaR extracellular domain. Functional characterization of these antibodies included Ca2+ stimulation of phosphoinositide hydrolysis to examine effects of intact or protease digested antibodies on sensitivity of the receptor to extracellular Ca2+ and flow cytometry assay of binding of the antibodies to HEK-293 cells expressing chimeric receptors to map antibody epitopes. Results: We found these mAbs specifically recognize native but not denatured human CaR or homologous native Fugu CaR. Sensitivity of the human CaR to extracellular calcium was increased by binding of 5C8 but decreased by binding of 1A8. A chimeric receptor FCFCF, with lobe 2 region of the human CaR VFT domain in the Fugu CaR backbone, bound all three mAbs, and the sensitivity of this chimeric CaR to extracellular Ca2+, was also increased by binding of 5CS and decreased by binding of 1A8. Conclusions: The epitopes of these mAbs reside in the lobe 2 region of the human CaR VFT domain. 5C8 might activate the receptor by facilitating closure and/or rotation of the VFT domains on agonist binding, whereas 1A8 might inhibit the receptor by impeding such agonist-induced conformational changes. Recombinant antibodies with antigen binding domains of 5C8 and 1A8 could be useful in the treatment of hyperparathyroidism and osteoporosis, respectively. C1 NIDCD, Mol Pathophysiol Sect, NIH, Bethesda, MD 20892 USA. NIDDK, Mol Med Branch, NIH, Bethesda, MD USA. RP Hu, JX (reprint author), NIDCD, Mol Pathophysiol Sect, NIH, Bldg 10,Room 8C-101,9000 Rockville Pike, Bethesda, MD 20892 USA. EM jianxinh@intra.niddk.nih.gov FU Intramural NIH HHS NR 15 TC 9 Z9 9 U1 0 U2 0 PU AMER SOC BONE & MINERAL RES PI WASHINGTON PA 2025 M ST, N W, STE 800, WASHINGTON, DC 20036-3309 USA SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD APR PY 2007 VL 22 IS 4 BP 601 EP 608 DI 10.1359/JBMR.070111 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 150RK UT WOS:000245234000014 PM 17243861 ER PT J AU Stefanovic, B Schwindt, W Hoehn, M Silva, AC AF Stefanovic, Bojana Schwindt, Wolfram Hoehn, Mathias Silva, Afonso C. TI Functional uncoupling of hemodynamic from neuronal response by inhibition of neuronal nitric oxide synthase SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article DE brain; functional activation; nitric oxide; 7-nitroindazole; fMRI ID CEREBRAL-BLOOD-FLOW; SOMATOSENSORY-EVOKED POTENTIALS; CYTOCHROME-C-OXIDASE; NMDA-INDUCED CHANGES; RAT-BRAIN; FOREPAW STIMULATION; CEREBROVASCULAR RESPONSE; VIBRISSAL STIMULATION; FREQUENCY-DEPENDENCE; OXYGEN-CONSUMPTION AB The cerebrovascular coupling under neuronal nitric oxide synthase (nNOS) inhibition was investigated in alpha-chloralose anesthetized rats. Cerebral blood flow (CBF), cerebral blood volume (CBV), and blood oxygenation level dependent (BOLD) responses to electrical stimulation of the forepaw were measured before and after an intraperitoneal bolus of 7-nitroindazole (7-NI), an in vivo inhibitor of the neuronal isoform of nitric oxide synthase. Neuronal activity was measured by recording somatosensory-evoked potentials (SEPs) via intracranial electrodes. 7-Nitroindazole produced a significant attenuation of the activation-elicited CBF (P < 10(-6)), CBV (P < 10(-6)), and BOLD responses (P < 10(-6)), without affecting the baseline perfusion level. The average Delta CBF was nulled, while Delta BOLD and Delta CBV decreased to similar to 30% of their respective amplitudes before 7-NI administration. The average SEP amplitude decreased (P < 10(-5)) to similar to 60% of its pretreatment value. These data describe a pharmacologically induced uncoupling between neuronal and hemodynamic responses to functional activation, and provide further support for the critical role of neuronally produced NO in the cerebrovascular coupling. C1 NINDS, LFMI, Cerebral Microcirculat Unit, NIH, Bethesda, MD 20892 USA. Univ Hosp Munster, Dept Clin Radiol, Munster, Germany. Max Planck Inst Neurol Res, In Vivo NMR Lab, Cologne, Germany. RP Stefanovic, B (reprint author), NINDS, LFMI, Cerebral Microcirculat Unit, NIH, 10 Ctr Dr,Bldg 10,Room B1D10, Bethesda, MD 20892 USA. EM stefanovicb@ninds.nih.gov RI Silva, Afonso/A-7129-2009 FU Intramural NIH HHS NR 89 TC 37 Z9 40 U1 1 U2 1 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD APR PY 2007 VL 27 IS 4 BP 741 EP 754 DI 10.1038/sj.jcbfm.9600377 PG 14 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA 152JR UT WOS:000245358800009 PM 16883353 ER PT J AU Daly, JW Wilham, JM Spande, TF Garraffo, HM Gil, RR Silva, GL Vaira, M AF Daly, J. W. Wilham, J. M. Spande, T. F. Garraffo, H. M. Gil, R. R. Silva, G. L. Vaira, M. TI Alkaloids in bufonid toads (Melanophryniscus): Temporal and geographic determinants for two Argentinian species SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE alkaloids; ants; arthropods; bufonid toads; mites ID POISON FROGS DENDROBATIDAE; SKIN ALKALOIDS; IMIDAZOLE-ALKYLAMINES; PHENYL-ALKYLAMINES; INDOLE-ALKYLAMINES; DIETARY ALKALOIDS; ARTHROPOD SOURCE; AMPHIBIAN SKIN; PUMILIOTOXINS; DECAHYDROQUINOLINES AB Bufonid toads of the genus Melanophryniscus represent one of several lineages of anurans with the ability to sequester alkaloids from dietary arthropods for chemical defense. The alkaloid profile for Melanophryniscus stelzneri from a location in the province of Cordoba, Argentina, changed significantly over a 10-year period, probably indicating changes in availability of alkaloid-containing arthropods. A total of 29 alkaloids were identified in two collections of this population. Eight alkaloids were identified in M. stelzneri from another location in the province of Cordoba. The alkaloid profiles of Melanophryniscus rubriventris collected from four locations in the provinces of Salta and Jujuy, Argentina, contained 44 compounds and differed considerably between locations. Furthermore, alkaloid profiles of M stelzneri and M rubriventris strongly differed, probably reflecting differences in the ecosystem and hence in availability of alkaloid-containing arthropods. C1 NIDDK, Bioorgan Chem Lab, NIH, DHHS, Bethesda, MD 20892 USA. Univ Nacl Cordoba, Dept Quim Organ, RA-5000 Cordoba, Argentina. Univ Nacl Salta, Museo Ciencias Nat, RA-4400 Salta, Argentina. Univ Nacl Jujuy, Fac Ingn, CIBA, San Salvador De Jujuy, Argentina. RP Daly, JW (reprint author), NIDDK, Bioorgan Chem Lab, NIH, DHHS, Bethesda, MD 20892 USA. EM jdaly@nih.gov RI Vaira, Marcos/B-3760-2012; Gil, Roberto/A-4758-2008; OI Vaira, Marcos/0000-0002-3651-5108; Gil, Roberto/0000-0002-8810-5047; Silva, Gloria/0000-0002-0763-8096 FU Intramural NIH HHS NR 36 TC 26 Z9 28 U1 0 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD APR PY 2007 VL 33 IS 4 BP 871 EP 887 DI 10.1007/s10886-007-9261-x PG 17 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 153LS UT WOS:000245437600016 PM 17333373 ER PT J AU Boocock, DJ Patel, KR Faust, GES Normolle, DP Marczylo, TH Crowell, JA Brenner, DE Booth, TD Gescher, A Steward, WP AF Boocock, David J. Patel, Ketan R. Faust, Guy E. S. Normolle, Daniel P. Marczylo, Timothy H. Crowell, James A. Brenner, Dean E. Booth, Tristan D. Gescher, Andreas Steward, William P. TI Quantitation of trans-resveratrol and detection of its metabolites in human plasma and urine by high performance liquid chromatography SO JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES LA English DT Article DE resveratrol; conjugated metabolites; HPLC; human; plasma; urine ID RED-WINE; INTESTINAL TUMORIGENESIS; RAT; BIOAVAILABILITY; ABSORPTION; PHYTOALEXIN; EXPRESSION; GRAPES AB We describe a reversed-phase HPLC method that uses gradient elution and UV detection (325 nm) to determine levels of resveratrol and identify six major conjugated metabolites in the plasma and urine of human volunteers after administration of a single oral dose of I g. Waters Atlantis C-18 3 mu m served as the stationary phase. The gradient was formed using ammonium acetate and methanol, containing 2% propan-2-ol. Detection is linear between 5 ng/mL and 500 ng/mL in plasma (5-1000 ng/mL in urine). The coefficient of variation for intra- and inter-day variation is <10%. The average recovery of resveratrol from plasma and urine is 58 +/- 3%. The data presented in this report demonstrate a rapid, sensitive and accurate method for the analysis of resveratrol and its metabolites in human plasma and urine for pharmacokinetic studies. (c) 2006 Elsevier B.V. All rights reserved. C1 Leicester Royal Infirm, Canc Biomakers & Prevent Grp, Dept Canc Studies & Mol Med, Leicester LE2 7LX, Leics, England. Univ Michigan, Sch Med, Dept Internal Med, Ann Arbor, MI 48109 USA. Univ Michigan, Sch Med, Dept PharmacolMed, Ann Arbor, MI 48109 USA. VA Med Ctr, Ann Arbor, MI USA. Univ Michigan, Sch Med, Dept Radiat Oncol, Ann Arbor, MI 48109 USA. Univ Michigan, Sch Med, Comprehens Canc Ctr, Ann Arbor, MI 48109 USA. Royalmount Pharma, Montreal, PQ H4P 2T4, Canada. NCI, Div Canc Prevent, Bethesda, MD 20892 USA. RP Boocock, DJ (reprint author), Leicester Royal Infirm, Canc Biomakers & Prevent Grp, Dept Canc Studies & Mol Med, 5th Floor RKCSB, Leicester LE2 7LX, Leics, England. EM djb27@le.ac.uk RI Boocock, David/I-4666-2015; OI Boocock, David/0000-0002-7333-3549; Normolle, Daniel/0000-0001-8675-5014 FU NCI NIH HHS [N01 CN025025, N01-CN-25025-3] NR 29 TC 96 Z9 98 U1 0 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1570-0232 J9 J CHROMATOGR B JI J. Chromatogr. B PD APR 1 PY 2007 VL 848 IS 2 BP 182 EP 187 DI 10.1016/j.jchromb.2006.10.017 PG 6 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 158AH UT WOS:000245762600002 PM 17097357 ER PT J AU Peoples, MC Phillips, TM Karnes, HT AF Peoples, Michael C. Phillips, Terry M. Karnes, H. Thomas TI A capillary-based microfluidic instrument suitable for immunoaffinity chromatography SO JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES LA English DT Article DE microflluidics; immunoglobulins; immunoaffinity; laser induced fluorescence; capillary chromatography ID MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; ELECTROPHORESIS; IMMUNOASSAYS; MICROCHIP; COLUMNS; SYSTEM; IMMUNOEXTRACTION; SEPARATION; SAMPLES AB The analysis of biological samples to produce clinical or research data often requires measurement of analytes from complex biological matrices and limited volumes. Miniaturized analytical systems capable of minimal sample consumption and reduced analysis times have been employed to meet this need. The small footprint of this technology offers the potential for portability and patient point-of-care testing. A prototype microfluidic, system has been developed and is presented for potential rapid assessment of clinical samples. The system has been designed for immunoaffinity chromatography as a means of separating analytes of interest from biological matrices. The instrument is capable of sub-microliter sample injection and detection of labeled antigens by long wavelength laser-induced fluorescence (LIF). The laboratory-constructed device is assembled from an array of components including two syringe pumps, a nano-gradient mixing chip, a micro-injector, a diode laser, and a separation capillary column made from a polymer/silica (PEEKsil) tube. An in-house program written with LabVIEW software controls the syringe pumps to perform step gradient elution and collects the LIF signal as a chromatogram. Initial columns were packed with silica beads to evaluate the system. Optimization of the device has been achieved by measuring flow accuracy with respect to column length and particle size. Syringe size and pressure effects have also been used to characterize the capability of the pumps. Based on test results, a 200-mu m x 25-mm column packed with 1-mu m silica beads was chosen for use with a 500-mu L syringe. The system was tested for mixer proportioning by pumping different compositions of buffer and fluorescent dye solutions in a stepwise fashion. A linear response was achieved for increasing concentrations of fluorescent dye by online mixing (R(2) = 0.9998). The effectiveness of an acidic gradient was confirmed by monitoring pH post-column and measuring premixed solutions offline. Finally, assessment of delectability was achieved by injecting fluorescent dye solutions and measuring the signal from the LIF detector. The limit of detection for the system with these solutions was 10.0 pM or 10.0 amol on-column. As proof-of-principle, immunoaffinity chromatography was demonstrated with immobilized rabbit anti-goat IgG and a fluorescent dye-goat IgG conjugate as a model antigen. (c) 2006 Elsevier B.V. All rights reserved. C1 Virginia Commonwealth Univ, Ctr Med, Dept Pharmaceut, Richmond, VA 23298 USA. NIH, Off Res Serv, Div Bioengn & Phys Sci, Ultramicro Analyt Immunochem Resource, Bethesda, MD 20892 USA. RP Karnes, HT (reprint author), Virginia Commonwealth Univ, Ctr Med, Dept Pharmaceut, POB 980533, Richmond, VA 23298 USA. EM tom.karnes@vcu.edu NR 37 TC 10 Z9 10 U1 6 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1570-0232 J9 J CHROMATOGR B JI J. Chromatogr. B PD APR 1 PY 2007 VL 848 IS 2 BP 200 EP 207 DI 10.1016/j.jchromb.2006.10.032 PG 8 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 158AH UT WOS:000245762600004 PM 17097929 ER PT J AU Scholz, T Eisenhofer, G Pacak, K Dralle, H Lehnert, H AF Scholz, Tim Eisenhofer, Graeme Pacak, Karel Dralle, Henning Lehnert, Hendrik TI Current treatment of malignant pheochromocytoma SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Review ID NEURAL CREST TUMORS; I-131 META-IODOBENZYLGUANIDINE; ADVANCED NEUROENDOCRINE TUMORS; METHYL-P-TYROSINE; COMBINATION CHEMOTHERAPY; RADIOPHARMACEUTICAL TREATMENT; METASTATIC PHEOCHROMOCYTOMA; FAMILIAL PHEOCHROMOCYTOMA; OCTREOTIDE SCINTIGRAPHY; BENIGN PHEOCHROMOCYTOMA AB Context: Pheochromocytomas are rare tumors of predominantly adrenal origin that often produce and secrete catecholamines. Malignancy occurs in a variable percentage of cases depending on genetic background and tumor location. Definitive diagnosis relies on the detection of distant metastases. Treatments for malignant pheochromocytoma include surgical debulking, pharmacological control of hormone-mediated symptoms, targeted methods such as external irradiation, and systemic antineoplastic therapy. Different agents and protocols for this purpose are reviewed, and their therapeutic potential is discussed. Evidence Acquisition: Literature on antineoplastic therapies for malignant pheochromocytoma was identified by searching the PubMed database with restriction to articles published in English during the past 30 yr. Evidence Synthesis: Because of the rarity of the condition, no randomized clinical trials concerning the treatment of malignant pheochromocytoma have been performed. The strategy established best is [(131)I]meta-iodobenzylguanidine (MIBG) therapy, which is well tolerated. Similar to cytotoxic chemotherapy with cyclophosphamide, vincristine, and dacarbazine, MIBG can induce remission for a limited period in a significant proportion of patients. Octreotide as a single agent seems to be largely ineffective. Conclusions: MIBG radiotherapy and cyclophosphamide, vincristine, and dacarbazine chemotherapy are comparable with respect to response rate and toxicity. It is unclear whether combining both can improve the outcome. Future developments may include new multimodal concepts with focus on inhibition of angiogenetic factors and heat shock protein 90. Any present or new therapeutic approach must take into account the highly variable natural course of the disease. C1 Otto Von Guericke Univ, Sch Med, Dept Endocrinol & Metab, D-39120 Magdeburg, Germany. Univ Hosp, Warwick Med Sch, Coventry CV4 7AL, W Midlands, England. NIH, Bethesda, MD 20892 USA. Univ Halle Wittenberg, Dept Gen Visceral & Vasc Surg, D-06099 Halle, Germany. RP Lehnert, H (reprint author), Univ Warwick, Clin Sci Res Inst, Chair Med, Med Sch Bldg,Gibbet Hill Campus, Coventry CV4 7AL, W Midlands, England. EM H.Lehnert@warwick.ac.uk NR 117 TC 86 Z9 95 U1 0 U2 7 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD APR PY 2007 VL 92 IS 4 BP 1217 EP 1225 DI 10.1210/jc.2006-1544 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 153WH UT WOS:000245466600005 PM 17284633 ER PT J AU Simonds, WF AF Simonds, William F. TI Ruling out a suspect: the role of beta-catenin mutation in benign parathyroid neoplasia SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Editorial Material ID WNT; TRANSCRIPTION; ACCUMULATION; ASSOCIATION; TUMORS; GENE C1 NIDDK, Metab Dis Branch, Endocrine Signaling & Oncogenesis Sect, NIH, Bethesda, MD 20892 USA. RP Simonds, WF (reprint author), NIDDK, Metab Dis Branch, Endocrine Signaling & Oncogenesis Sect, NIH, Bldg 10,Room 8C-101,MSC 1752, Bethesda, MD 20892 USA. EM wfs@helix.nih.gov FU Intramural NIH HHS NR 12 TC 3 Z9 3 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD APR PY 2007 VL 92 IS 4 BP 1235 EP 1236 DI 10.1210/jc.2007-0314 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 153WH UT WOS:000245466600009 PM 17409343 ER PT J AU Florez, JC Jablonski, KA Sun, MW Bayley, N Kahn, SE Shamoon, H Hamman, RF Knowler, WC Nathan, DM Altshuler, D AF Florez, Jose C. Jablonski, Kathleen A. Sun, Maria W. Bayley, Nick Kahn, Steven E. Shamoon, Harry Hamman, Richard F. Knowler, William C. Nathan, David M. Altshuler, David CA Diabetes Preventio TI Effects of the type 2 diabetes-associated PPARG P12A polymorphism on progression to diabetes and response to troglitazone SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID PROLIFERATOR-ACTIVATED RECEPTOR-GAMMA-2; IMPAIRED GLUCOSE-TOLERANCE; LIFE-STYLE INTERVENTION; PRO12ALA POLYMORPHISM; PPAR-GAMMA-2 GENE; PREVENTION PROGRAM; INSULIN-SECRETION; GAMMA-GENE; BODY-MASS; POPULATION AB Context: The common P12A polymorphism in PPARG (a target for thiazolidinedione medications) has been consistently associated with type 2 diabetes. Objective: We examined whether PPARG P12A affects progression from impaired glucose tolerance to diabetes, or responses to preventive interventions (lifestyle, metformin, or troglitazone vs. placebo). Patients: This study included 3548 Diabetes Prevention Program participants. Design: We performed Cox regression analysis using genotype at PPARG P12A, intervention, and their interactions as predictors of diabetes incidence. We also genotyped five other PPARG variants implicated in the response to troglitazone and assessed their effect on insulin sensitivity at 1 yr. Results: Consistent with prior cross-sectional studies, P/P homozygotes at PPARG P12A appeared more likely to develop diabetes than alanine carriers (hazard ratio, 1.24; 95% confidence interval, 0.99-1.57; P = 0.07) with no interaction of genotype with intervention. There was a significant interaction of genotype with body mass index and waist circumference (P = 0.03 and 0.002, respectively) with the alanine allele conferring less protection in more obese individuals. Neither PPARG P12A nor five other variants significantly affected the impact of troglitazone on insulin sensitivity in 340 participants at 1 yr. Conclusions: The proline allele at PPARG P12A increases risk for diabetes in persons with impaired glucose tolerance, an effect modified by body mass index. In addition, PPARG P12A has little or no effect on the beneficial response to troglitazone. C1 George Washington Univ, Diabet Prevent Program Coordinating Ctr, Biostat Ctr, Rockville, MD 20852 USA. Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA. Massachusetts Gen Hosp, Dept Med, Boston, MA 02114 USA. Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA. Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA. MIT, Cambridge, MA 02139 USA. Harvard Univ, Broad Inst, Cambridge, MA 02138 USA. Univ Washington, Seattle, WA 98108 USA. VA Puget Sound Hlth Care Syst, Div Metab Endocrinol & Nutr, Dept Med, Seattle, WA USA. Albert Einstein Coll Med, Div Endocrinol & Metab, Dept Med, Bronx, NY 10461 USA. Univ Colorado Denver & Hlth Sci Ctr, Dept Prevent Med & Biometr, Denver, CO 80262 USA. NIDDKD, Diabet Epidemiol & Clin Res Sect, Phoenix, AZ 85014 USA. RP Florez, JC (reprint author), George Washington Univ, Diabet Prevent Program Coordinating Ctr, Biostat Ctr, 6110 Execut Blvd,Suite 750, Rockville, MD 20852 USA. EM jcflorez@partners.org RI Altshuler, David/A-4476-2009; OI Altshuler, David/0000-0002-7250-4107; Shamoon, Harry/0000-0002-5014-5211; Kahn, Steven/0000-0001-7307-9002 FU NIDDK NIH HHS [U01 DK048489, 1 K23 DK65978-03, U01 DK048489-06, K23 DK065978] NR 42 TC 84 Z9 88 U1 0 U2 2 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD APR PY 2007 VL 92 IS 4 BP 1502 EP 1509 DI 10.1210/jc.2006-2275 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 153WH UT WOS:000245466600052 PM 17213274 ER PT J AU Sadakata, T Washida, M Iwayama, Y Shoji, S Sato, Y Ohkura, T Katoh-Semba, R Nakajima, M Sekine, Y Tanaka, M Nakamura, K Iwata, Y Tsuchiya, KJ Mori, N Detera-Wadleigh, SD Ichikawa, H Itohara, S Yoshikawa, T Furuichi, T AF Sadakata, Tetsushi Washida, Miwa Iwayama, Yoshimi Shoji, Satoshi Sato, Yumi Ohkura, Takeshi Katoh-Semba, Ritsuko Nakajima, Mizuho Sekine, Yukiko Tanaka, Mika Nakamura, Kazuhiko Iwata, Yasuhide Tsuchiya, Kenji J. Mori, Norio Detera-Wadleigh, Sevilla D. Ichikawa, Hironobu Itohara, Shigeyoshi Yoshikawa, Takeo Furuichi, Teiichi TI Autistic-like phenotypes in Cadps2-knockout mice and aberrant CADPS2 splicing in autistic patients SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article ID PERVASIVE DEVELOPMENTAL DISORDERS; CORE VESICLE EXOCYTOSIS; CA2+-DEPENDENT ACTIVATOR; SUSCEPTIBILITY LOCUS; REDUCED EXPLORATION; NEUROTROPHIC FACTOR; MENTAL-RETARDATION; NEONATAL BLOOD; GENOME SCAN; PROTEIN AB Autism, characterized by profound impairment in social interactions and communicative skills, is the most common neurodevelopmental disorder, and its underlying molecular mechanisms remain unknown. Ca2+-dependent activator protein for secretion 2 (CADPS2; also known as CAPS2) mediates the exocytosis of densecore vesicles, and the human CADPS2 is located within the autism susceptibility locus 1 on chromosome 7q. Here we show that Cadps2-knockout mice not only have impaired brain-derived neurotrophic factor release but also show autistic-like cellular and behavioral phenotypes. Moreover, we found an aberrant alternatively spliced CADPS2 mRNA that lacks exon 3 in some autistic patients. Exon 3 was shown to encode the dynactin 1-binding domain and affect axonal CADPS2 protein distribution. Our results suggest that a disturbance in CADPS2-mediated neurotrophin release contributes to autism susceptibility. C1 RIKEN, Brain Sci Inst, Lab Mol Neurogenesis, Wako, Saitama 3510198, Japan. RIKEN, Brain Sci Inst, Lab Mol Psychiat, Wako, Saitama 3510198, Japan. Tokyo Metropolitan Umegaoka Hosp, Tokyo, Japan. Aichi Human Serv Ctr, Inst Dev Res, Dept Perinatol, Kasugai, Aichi, Japan. RIKEN, Brain Sci Inst, Res Resource Ctr, Wako, Saitama 3510198, Japan. Hamamatsu Univ Sch Med, Dept Psychiat & Neurol, Hamamatsu, Shizuoka 43131, Japan. NIMH, Mood & Anxiety Disorders Program, Bethesda, MD 20892 USA. RIKEN, Brain Sci Inst, Lab Behav Genet, Wako, Saitama 3510198, Japan. RP Furuichi, T (reprint author), RIKEN, Brain Sci Inst, Lab Mol Neurogenesis, Wako, Saitama 3510198, Japan. EM tfuruichi@brain.riken.jp RI Itohara, Shigeyoshi/I-8769-2012; OI Itohara, Shigeyoshi/0000-0002-2410-9989; Tsuchiya, Kenji/0000-0002-1314-4199; Sadakata, Tetsushi/0000-0003-0276-4162 NR 62 TC 99 Z9 103 U1 3 U2 13 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD APR PY 2007 VL 117 IS 4 BP 931 EP 943 DI 10.1172/JCI29031 PG 13 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 153RC UT WOS:000245451700015 PM 17380209 ER PT J AU Chiang, JY Jang, IK Hodes, R Gu, H AF Chiang, Jeffrey Y. Jang, Ihn Kyung Hodes, Richard Gu, Hua TI Ablation of Cbl-b provides protection against transplanted and spontaneous tumors SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article ID T-CELL-ACTIVATION; NEGATIVE REGULATOR; C-CBL; COSTIMULATION; LYMPHOCYTES; IMMUNOTHERAPY; REJECTION; ANTIGENS; IMMUNITY; LIGASE AB A significant challenge to efforts aimed at inducing effective antitumor immune responses is that CD8(+) T cells, which play a prominent role in these responses, may be unable to respond to tumors that lack costimulatory signals and that are protected by an immune suppressive environment such as that mediated by TGF-beta produced by tumor cells themselves or by infiltrating Tregs, often resulting in tolerance or anergy of tumor-specific T cells. Here we show that the in vitro activation of Cb1b(-/-) CD8(+) T cells does not depend on CD28 costimulation and is resistant to TGF-beta suppression. In vivo studies further demonstrated that Cb1b(-/-) mice, but not WT controls, efficiently rejected inoculated E.G7 and EL4 lymphomas that did not express 137 ligands and that introduction of the Cb1b(-/-) mutation into tumor-prone ataxia telangiectasia mutated-deficient mice markedly reduced the incidence of spontaneous thymic lymphomas. Immunohistological study showed that E.G7 tumors from Cblb(-/-) mice contained massively infiltrating CD8(+) T cells. Adoptive transfer of purified Cblb(-/-) CD8(+) T cells into E.G7 tumor-bearing mice led to efficient eradication of established tumors. Thus, our data indicate that ablation of Cb1-b can be an efficient strategy for eliciting immune responses against both inoculated and spontaneous tumors. C1 Columbia Univ Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA. NCI, Expt Immunol Branch, NIH, Bethesda, MD USA. NIA, NIH, Bethesda, MD USA. RP Gu, H (reprint author), Columbia Univ Coll Phys & Surg, Dept Microbiol, 701 W 168th St,HHSC 610, New York, NY 10032 USA. EM richard.hodes@nih.hhs.gov FU Intramural NIH HHS NR 31 TC 52 Z9 58 U1 0 U2 1 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD APR PY 2007 VL 117 IS 4 BP 1029 EP 1036 DI 10.1172/JCI29472 PG 8 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 153RC UT WOS:000245451700025 PM 17364027 ER PT J AU Conville, PS Witebsky, FG AF Conville, Patricia S. Witebsky, Frank G. TI Analysis of multiple differing copies of the 16S rRNA gene in five clinical isolates and three type strains of Nocardia species and implications for species assignment SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID RIBOSOMAL-RNA GENE; RESTRICTION-ENDONUCLEASE ANALYSIS; SEQUENCE-BASED IDENTIFICATION; SP-NOV.; RAPID IDENTIFICATION; DNA; SPECIMENS; PATHOGEN; IGNORATA; LEVEL AB Five clinical isolates of Nocardia that showed ambiguous bases within the variable region of the 16S rRNA gene sequence were evaluated for the presence of multiple copies of this gene. The type strains of three Nocardia species, Nocardia concava, Nocardia ignorata, and Nocardia yamanashiensis, which also showed ambiguous bases in the variable region, were also examined. Cloning experiments using an amplified region of the 16S rRNA that contains the variable region showed that each isolate possessed 16S rRNA genes with at least two different sequences. In addition, hybridization studies using a 16S rRNA gene-specific probe and extracted genomic DNA of the patient isolates and of the type strain of N. ignorata showed that each isolate possessed at least three copies of the gene. These multiple differing copies of the 16S rRNA gene and the results of DNA-DNA hybridization studies indicate problems of species definition and identification for such isolates. A broader species concept than that currently in vogue may be required to accommodate such organisms. C1 NIH, Microbiol Serv, Dept Lab Med, Warren G Magnuson Clin Ctr,US Dept Hlth & Human S, Bethesda, MD 20892 USA. RP Conville, PS (reprint author), NIH, Microbiol Serv, Dept Lab Med, Warren G Magnuson Clin Ctr,US Dept Hlth & Human S, 10 Ctr Dr,MSC 1508, Bethesda, MD 20892 USA. EM pconville@cc.nih.gov FU Intramural NIH HHS NR 21 TC 24 Z9 26 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD APR PY 2007 VL 45 IS 4 BP 1146 EP 1151 DI 10.1128/JCM.02482-06 PG 6 WC Microbiology SC Microbiology GA 158GI UT WOS:000245779300011 PM 17301281 ER PT J AU Willi, B Filoni, C Catao-Dias, JL Cattori, V Meli, ML Vargas, A Martinez, F Roelke, ME Ryser-Degiorgis, MP Leutenegger, CM Lutz, H Hofmann-Lehmann, R AF Willi, Barbara Filoni, Claudia Catao-Dias, Jose L. Cattori, Valentino Meli, Marina L. Vargas, Astrid Martinez, Fernando Roelke, Melody E. Ryser-Degiorgis, Marie-Pierre Leutenegger, Christian M. Lutz, Hans Hofmann-Lehmann, Regina TI Worldwide occurrence of feline hernoplasma infections in wild felid species SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; CANDIDATUS MYCOPLASMA HAEMOMINUTUM; TIME PCR ASSAY; HAEMOBARTONELLA-FELIS; IMMUNODEFICIENCY VIRUS; UNITED-KINGDOM; EXPERIMENTAL TRANSMISSION; CTENOCEPHALIDES-FELIS; HAEMOPLASMA INFECTION; PHYLOGENETIC ANALYSIS AB While hemoplasma infections in domestic cats are well studied, almost no information is available on their occurrence in wild felids. The aims of the present study were to investigate wild felid species as possible reservoirs of feline hemoplasmas and the molecular characterization of the hemoplasma isolates. Blood samples from the following 257 wild felids were analyzed: 35 Iberian lynxes from Spain, 36 Eurasian lynxes from Switzerland, 31 European wildcats from France, 45 lions from Tanzania, and 110 Brazilian wild felids, including 12 wild felid species kept in zoos and one free-ranging ocelot. Using real-time PCR, feline hemoplasmas were detected in samples of the following species: Iberian lynx, Eurasian lynx, European wildcat, lion, puma, oncilla, Geoffroy's cat, margay, and ocelot. "Candidatus Mycoplasma haemominutum" was the most common feline hemoplasma in Iberian lynxes, Eurasian lynxes, Serengeti lions, and Brazilian wild felids, whereas "Candidatus Mycoplasma turicensis" was the most prevalent in European wildcats; hemoplasma coinfections were frequently observed. Hemoplasma infection was associated with species and free-ranging status of the felids in all animals and with feline leukemia virus provirus-positive status in European wildcats. Phylogenetic analyses of the 16S rRNA and the partial RNase P gene revealed that most hemoplasma isolates exhibit high sequence identities to domestic cat-derived isolates, although some isolates form different subclusters within the phylogenetic tree. In conclusion, 9 out of 15 wild felid species from three different continents were found to be infected with feline hemoplasmas. The effect of feline hemoplasma infections on wild felid populations needs to be further investigated. C1 Univ Zurich, Clin Lab, Vetsuisse Fac, CH-8057 Zurich, Switzerland. Univ Sao Paulo, Dept Patol, Fac Med Vet & Zootecn, Sao Paulo, Brazil. Fdn Parque Zool, Sao Paulo, Brazil. Ctr Cria Lince Iber, Matalascanas, Spain. NCI, Lab Genom Divers, SAIC Frederick Inc, Ft Detrick, MD 21702 USA. Univ Bern, Ctr Fish & Wildlife Hlth, Inst Anim Pathol, Vetsuisse Fac, Bern, Switzerland. Univ Calif Davis, Dept Med & Epidemiol, Davis, CA 95616 USA. RP Hofmann-Lehmann, R (reprint author), Univ Zurich, Clin Lab, Vetsuisse Fac, Winterthurerstr 260, CH-8057 Zurich, Switzerland. EM rhofmann@vetclinics.unizh.ch RI Hofmann-Lehmann, Regina/C-6528-2009; Catao-Dias, Jose Luiz/C-4897-2012; Filoni, Claudia /E-9780-2012 OI Catao-Dias, Jose Luiz/0000-0003-2999-3395; Filoni, Claudia /0000-0001-8819-2331 NR 38 TC 52 Z9 53 U1 2 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD APR PY 2007 VL 45 IS 4 BP 1159 EP 1166 DI 10.1128/JCM.02005-06 PG 8 WC Microbiology SC Microbiology GA 158GI UT WOS:000245779300013 PM 17301277 ER PT J AU Salaverria, I Zettl, A Bea, S Moreno, V Valls, J Hartmann, E Ott, G Wright, G Lopez-Guillermo, A Chan, WC Weisenburger, DD Gascoyne, RD Grogan, TM Delabie, J Jaffe, ES Montserrat, E Muller-Hermelink, HK Staudt, LM Rosenwald, A Campo, E AF Salaverria, Itziar Zettl, Andreas Bea, Silvia Moreno, Victor Valls, Joan Hartmann, Elena Ott, German Wright, George Lopez-Guillermo, Armando Chan, Wing C. Weisenburger, Dennis D. Gascoyne, Randy D. Grogan, Thomas M. Delabie, Jan Jaffe, Elaine S. Montserrat, Emili Muller-Hermelink, Hans-Konrad Staudt, Louis M. Rosenwald, Andreas Campo, Elias TI Specific secondary genetic alterations in mantle cell lymphoma provide prognostic information independent of the gene expression-based proliferation signature SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article; Proceedings Paper CT 95th Annual Meeting of the United-States-and-Canadian-Academy-of-Pathology CY FEB 11-17, 2006 CL Atlanta, GA SP US & Canadian Acad Pathol ID COMPARATIVE GENOMIC HYBRIDIZATION; CHRONIC LYMPHOCYTIC-LEUKEMIA; CHROMOSOMAL IMBALANCES; AGGRESSIVE VARIANTS; BREAST-CANCER; ATM GENE; DNA; MUTATIONS; OVEREXPRESSION; AMPLIFICATION AB Purpose To compare the genetic relationship between cyclin D1 - positive and cyclin D1 - negative mantle cell lymphomas (MCLs) and to determine whether specific genetic alterations may add prognostic information to survival prediction based on the proliferation signature of MCLs. Patients and Methods Seventy-one cyclin D1 - positive and six cyclin D1 - negative MCLs previously characterized by gene expression profiling were examined by comparative genomic hybridization (CGH). Results Cyclin D1 - negative MCLs were genetically characterized by gains of 3q, 8q, and 15q, and losses of 1p, 8p23- pter, 9p21- pter, 11q21- q23, and 13q that were also the most common alterations in conventional MCLs. Parallel analysis of CGH aberrations and locus-specific gene expression profiles in cyclin D1 - positive patients showed that chromosomal imbalances had a substantial impact on the expression levels of the genes located in the altered regions. The analysis of prognostic factors revealed that the proliferation signature, the number of chromosomal aberrations, gains of 3q, and losses of 8p, 9p, and 9q predicted survival of MCL patients. A multivariate analysis showed that the gene expression-based proliferation signature was the strongest predictor for shorter survival. However, 3q gains and 9q losses provided prognostic information that was independent of the proliferative activity. Conclusion Cyclin D1 - positive and - negative MCLs share the same secondary genetic aberrations, supporting the concept that they correspond to the same genetic entity. The integration of genetic information on chromosome 3q and 9q alterations into a proliferation signature-based model may improve the ability to predict survival in patients with MCL. C1 Univ Barcelona, Hosp Clin, Hematopathol Sect, Dept Pathol & Hematol, E-08036 Barcelona, Spain. Autonomous Univ Barcelona, Canc Epidemiol Serv, Inst Invest Biomed Bellvitge, Catalan Inst Oncol, Barcelona, Spain. Autonomous Univ Barcelona, Lab Estad & Epidemiol, Barcelona, Spain. Polytech Univ Barcelona, Dept Stat, ETSEIB, Barcelona, Spain. Univ Wurzburg, Dept Pathol, D-8700 Wurzburg, Germany. NCI, Biometr Res Metab & Pathol Branch, NIH, Bethesda, MD 20892 USA. NIH, Mol Anal Sect, Computat Biosci & Engn Lab, Ctr Informat Technol, Bethesda, MD 20892 USA. Univ Nebraska Med Ctr, Dept Pathol & Microbiol, Omaha, NE USA. Univ Nebraska Med Ctr, Dept Internal Med, Omaha, NE USA. Netherlands Canc Ctr, Dept Pathol, Amsterdam, Netherlands. Univ British Columbia, Dept Pathol, Vancouver, BC V5Z 1M9, Canada. Univ British Columbia, Div Med Oncol, Vancouver, BC V5Z 1M9, Canada. British Columbia Canc Agcy, Vancouver, BC V5Z 4E6, Canada. Oregon Hlth & Sci Univ, SW Oncol Grp, Portland, OR 97201 USA. Univ Arizona, Ctr Canc, Dept Pathol, Tucson, AZ USA. Univ Arizona, Ctr Canc, Dept Med, Tucson, AZ USA. Univ Rochester, Sch Med, James P Wilmot Canc Ctr, Rochester, NY USA. Rikshosp Radiumhosp, Dept Immunol, Med Ctr, Oslo, Norway. Rikshosp Radiumhosp, Dept Oncol, Med Ctr, Oslo, Norway. Rikshosp Radiumhosp, Dept Pathol, Med Ctr, Oslo, Norway. Univ Oslo, Oslo, Norway. RP Campo, E (reprint author), Univ Barcelona, Hosp Clin, Hematopathol Sect, Dept Pathol & Hematol, Villarroel 170, E-08036 Barcelona, Spain. EM ecampo@clinic.ub.es RI Moreno, Victor/A-1697-2010; SALAVERRIA, ITZIAR/L-2246-2015; Bea, Silvia/K-7699-2014; OI Moreno, Victor/0000-0002-2818-5487; SALAVERRIA, ITZIAR/0000-0002-2427-9822; Delabie, Jan/0000-0001-5023-0689; Bea, Silvia/0000-0001-7192-2385; Campo, elias/0000-0001-9850-9793 FU NCI NIH HHS [UO1-CA84967, U01 CA084967, U01 CA114778-01] NR 42 TC 103 Z9 108 U1 2 U2 3 PU AMER SOC CLINICAL ONCOLOGY PI ALEXANDRIA PA 330 JOHN CARLYLE ST, STE 300, ALEXANDRIA, VA 22314 USA SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD APR 1 PY 2007 VL 25 IS 10 BP 1216 EP 1222 DI 10.1200/JCO.2006.08.4251 PG 7 WC Oncology SC Oncology GA 156VF UT WOS:000245677300010 PM 17296973 ER PT J AU Coon, KD Myers, AJ Craig, DW Webster, JA Pearson, JV Lince, DH Zismann, VL Beach, TG Leung, D Bryden, L Halperin, RF Marlowe, L Kaleem, M Walker, DG Ravid, R Heward, CB Rogers, J Papassotiropoulos, A Reiman, EM Hardy, J Stephan, DA AF Coon, Keith D. Myers, Amanda J. Craig, David W. Webster, Jennifer A. Pearson, John V. Lince, Diane Hu Zismann, Victoria L. Beach, Thomas G. Leung, Doris Bryden, Leslie Halperin, Rebecca F. Marlowe, Lauren Kaleem, Mona Walker, Douglas G. Ravid, Rivka Heward, Christopher B. Rogers, Joseph Papassotiropoulos, Andreas Reiman, Eric M. Hardy, John Stephan, Dietrich A. TI A high-density whole-genome association study reveals that APOE is the major susceptibility gene for sporadic late-onset Alzheimer's disease SO JOURNAL OF CLINICAL PSYCHIATRY LA English DT Article ID POPULATION; GENOTYPE; ALLELE AB Objective: While the apolipoprotein E (APOE) epsilon 4 allele is a well-established risk factor for late-onset Alzheimer's disease (AD), initial genome scans using microsatellite markers in late-onset AD failed to identify this locus on chromosome 19. Recently developed methods for the simultaneous assessment of hundreds of thousands of single nucleotide polymorphisms (SNPs) promise to help more precisely identify loci that contribute to the risk of AD and other common multigenic conditions. We sought here to demonstrate that more precise identification of loci that are associated with complex, multigenic genetic disorders can be achieved using ultra-high-density whole-genome associations by demonstrating their ability to identify the APOE locus as a major susceptibility gene for late-onset AD, despite the absence of SNPs within the APOE locus itself, as well as to refine odds ratios (ORs) based on gold-standard phenotyping of the study population. Method: An individualized genome-wide association study using 502,627 SNPs was performed in 1086 histopathologically verified AD cases and controls to determine the OR associated with genes predisposing to Alzheimer's disease. Results: As predicted, ultra-high-density SNP genotyping, in contrast to traditional microsatellite-based genome screening approaches, precisely identified the APOE locus as having a significant association with late-onset AD. SNP rs4420638 on chromosome 19, located 14 kilobase pairs distal to the APOE epsilon 4 variant, significantly distinguished between AD cases and controls (Bonferroni corrected p value = 5.30 x 10(-34), OR = 4.01) and was far more strongly associated with the risk of AD than any other SNP of the 502,627 tested. Conclusion: This study provides empirical support for the suggestion that the APOE locus is the major susceptibility gene for late-onset AD in the human genome, with an OR significantly greater than any other locus in the human genome. It also supports the feasibility of the ultra-high-density whole-genome association approach to the study of AD and other heritable phenotypes. These whole-genome association studies show great promise to identify additional genes that contribute to the risk of AD. C1 TGen Translat Genom Res Inst, Neurogenom Div, Phoenix, AZ 85004 USA. Univ Miami, Miller Sch Med, Dept Psychiat & Behav Sci, Miami, FL 33152 USA. Sun Hlth Res Inst, Sun City, AZ USA. NIA, Neurogenet Lab, NIH, Bethesda, MD 20892 USA. Royal Dutch Acad Sci, Amsterdam, Netherlands. Kronos Sci Labs, Phoenix, AZ USA. Univ Zurich, Div Psychiat Res, Zurich, Switzerland. RP Stephan, DA (reprint author), TGen Translat Genom Res Inst, Neurogenom Div, 400 N 5th St,Suite 1600, Phoenix, AZ 85004 USA. EM dstephan@tgen.org RI Myers, Amanda/B-1796-2010; Hardy, John/C-2451-2009; Pearson, John/F-2249-2011; Leung, Doris/R-5447-2016 OI Myers, Amanda/0000-0002-3100-9396; Pearson, John/0000-0003-0904-4598; Leung, Doris/0000-0002-2558-7798 FU Medical Research Council [G0701075]; NIA NIH HHS [U01 AG016976] NR 18 TC 271 Z9 280 U1 5 U2 29 PU PHYSICIANS POSTGRADUATE PRESS PI MEMPHIS PA P O BOX 240008, MEMPHIS, TN 38124 USA SN 0160-6689 J9 J CLIN PSYCHIAT JI J. Clin. Psychiatry PD APR PY 2007 VL 68 IS 4 BP 613 EP 618 PG 6 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA 159OP UT WOS:000245876000018 PM 17474819 ER PT J AU Olariu, A Cleaver, KM Cameron, HA AF Olariu, Ana Cleaver, Kathryn M. Cameron, Heather A. TI Decreased neurogenesis in aged rats results from loss of granule cell precursors without lengthening of the cell cycle SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE cell cycle; dentate gyrus; aging; adult; precursor cells ID ADULT DENTATE GYRUS; HIPPOCAMPAL NEUROGENESIS; DNA-REPLICATION; NUCLEAR ANTIGEN; SPATIAL MEMORY; OLD-AGE; PROLIFERATION; NEURONS; ZONE; ARCHITECTURE AB It is well established that neurogenesis in the dentate gyrus slows with aging, but it is unclear whether this change is due to slowing of the cell cycle, as occurs during development, or to loss of precursor cells. In the current study, we find that the cell cycle time of granule cell precursors in middle-aged male rats is not significantly different from that in young adults. The size of the precursor pool, however, was 3-4 times smaller in the middle-aged rats, as determined using both cumulative bromodeoxyuridine (BrdU) labeling as well as labeling with the endogenous marker of cell proliferation, proliferating cell nuclear antigen (PCNA). Loss of precursor cells was much greater in the granule cell layer than in the hilus, suggesting that dividing cells in the hilus belong to a distinct population, most likely glial progenitors, that are less affected by aging than neuronal precursors. BrdU-labeled precursor cells and young neurons, labeled with doublecortin, appeared to be lost equally from rostral and caudal, as well as suprapyramidal and infrapyramidal, subregions of the granule cell layer. However, doublecortin staining did show large parts of the caudal granule cell layer with few if any young neurons at both ages. Taken together, these findings indicate that precursor cells are not distributed evenly within the dentate gyrus in adulthood but that precursors are lost from throughout the dentate gyrus in old age with no concomitant change in the cell cycle time. C1 NIMH, Unit Neuroplast, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Cameron, HA (reprint author), NIMH, Unit Neuroplast, NIH, Dept Hlth & Human Serv, 35-3C915, Bethesda, MD 20892 USA. EM heathercameron@mail.nih.gov RI Cameron, Heather/E-6221-2011 OI Cameron, Heather/0000-0002-3245-5777 FU Intramural NIH HHS; NIMH NIH HHS [Z01-MH002784] NR 33 TC 82 Z9 87 U1 0 U2 7 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD APR 1 PY 2007 VL 501 IS 4 BP 659 EP 667 DI 10.1002/cne.21268 PG 9 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA 141BD UT WOS:000244550400013 PM 17278139 ER PT J AU Gibson, CW Yuan, ZA Li, Y Daly, B Suggs, C Aragon, MA Alawi, F Kulkarni, AB Wright, JT AF Gibson, C. W. Yuan, Z. A. Li, Y. Daly, B. Suggs, C. Aragon, M. A. Alawi, F. Kulkarni, A. B. Wright, J. T. TI Transgenic mice that express normal and mutated amelogenins SO JOURNAL OF DENTAL RESEARCH LA English DT Article DE dental enamel; amelogenin; transgenic mice; odontogenic tumor ID ENAMEL-MATRIX; GENE-EXPRESSION; IMPERFECTA; PROTEINS; PHENOTYPE; DIFFERENTIATION; AMELOBLASTIN; CLONING; DESIGN; NULL AB Amelogenin proteins are secreted by ameloblasts within the enamel organ during tooth development. To better understand the function of the 180-amino-acid amelogenin (M180), and to test the hypothesis that a single proline-tothreonine (P70T) change would lead to an enamel defect similar to amelogenesis imperfecta (AI) in humans, we generated transgenic mice with expression of M180, or M180 with the proline-tothreonine ( P70T) mutation, under control of the Amelx gene regulatory regions. M180 teeth had a relatively normal phenotype; however, P70T mineral was abnormally porous, with aprismatic regions similar to those in enamel of male amelogenesis imperfecta patients with an identical mutation. When Amelx null females were mated with P70T transgenic males, offspring developed structures similar to calcifying epithelial odontogenic tumors in humans. The phenotype argues for dominant-negative activity for the P70T amelogenin, and for the robust nature of the process of amelogenesis. C1 Univ Penn, Sch Dent Med, Dept Anat & Cell Biol, Philadelphia, PA 19104 USA. Univ Penn, Sch Dent Med, Dept Pathol, Philadelphia, PA 19104 USA. Univ N Carolina, Sch Dent, Dept Pediat Dent, Chapel Hill, NC 27599 USA. Natl Inst Dent & Craniofacial Res, Funct Genom Sect, NIH, Bethesda, MD 20892 USA. RP Gibson, CW (reprint author), Univ Penn, Sch Dent Med, Dept Anat & Cell Biol, 240 S 40th St, Philadelphia, PA 19104 USA. EM gibson@biochem.dental.upenn.edu FU Intramural NIH HHS; NIDCR NIH HHS [DE11089] NR 30 TC 21 Z9 21 U1 0 U2 1 PU INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314-3406 USA SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PD APR PY 2007 VL 86 IS 4 BP 331 EP 335 PG 5 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA 149XA UT WOS:000245179000006 PM 17384027 ER PT J AU Wiener, L Mellins, CA Marhefka, S Battles, HB AF Wiener, Lori Mellins, Claude Ann Marhefka, Stephanie Battles, Haven B. TI Disclosure of an HIV diagnosis to children: History, current research, and future directions SO JOURNAL OF DEVELOPMENTAL AND BEHAVIORAL PEDIATRICS LA English DT Article; Proceedings Paper CT Workshop on Disclosure of HIV Serostatus to Children and Adolescents CY APR 12-13, 2004 CL NY State Psychiat Inst, New York, NY HO NY State Psychiat Inst DE diagnostic disclosure; pediatric HIV; psychological distress; communication; systematic literature review ID HUMAN-IMMUNODEFICIENCY-VIRUS; SCHOOL-AGE-CHILDREN; INFECTED CHILDREN; PSYCHOLOGICAL ADJUSTMENT; PEDIATRIC-PATIENTS; OLDER CHILDREN; MENTAL-HEALTH; CANCER; ADOLESCENTS; ILLNESS AB Disclosing the diagnosis of human immunodeficiency virus (HIV) or AIDS to a child is a controversial and emotionally charged issue among both the health care communities and parents and caregivers of these children. This paper provides a systematic review of research on disclosure of pediatric HIV infection. It begins with a brief discussion of disclosure drawing from research on pediatric cancer. Next, we review the available research including patterns of disclosure, factors associated with disclosure and nondisclosure, and the effect of disclosure on psychological health and adherence. A review of published intervention studies is also included. While no consensus on when the diagnosis of HIV should be disclosed to a child or the psychological outcomes associated with disclosure was found, clinical consensus on several issues related to working with families was identified. We apply this literature to clinical practice and suggest avenues and directions for future research. C1 NCI, Pediat Oncol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. New York State Psychiat Inst & Hosp, HIV Ctr Clin & Behav Studies, New York, NY 10032 USA. Columbia Univ, New York, NY USA. RP Wiener, L (reprint author), 9000 Rockville Pike,10-SE Pediat Clin Room 6466, Bethesda, MD 20892 USA. EM wienerl@mail.nih.gov FU Intramural NIH HHS [Z99 CA999999]; NIMH NIH HHS [P30 MH43520, P30 MH043520] NR 71 TC 108 Z9 115 U1 3 U2 14 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0196-206X J9 J DEV BEHAV PEDIATR JI J. Dev. Behav. Pediatr. PD APR PY 2007 VL 28 IS 2 BP 155 EP 166 DI 10.1097/01.DBP.0000267570.87564.cd PG 12 WC Behavioral Sciences; Psychology, Developmental; Pediatrics SC Behavioral Sciences; Psychology; Pediatrics GA 158KG UT WOS:000245789800012 PM 17435473 ER PT J AU Nilsson, O Parker, EA Hegde, A Chau, M Barnes, KM Baron, J AF Nilsson, Ola Parker, Elizabeth A. Hegde, Anita Chau, Michael Barnes, Kevin M. Baron, Jeffrey TI Gradients in bone morphogenetic protein-related gene expression across the growth plate SO JOURNAL OF ENDOCRINOLOGY LA English DT Article ID ENDOCHONDRAL OSSIFICATION; RESTING ZONE; CHONDROGENESIS; BMP; DIFFERENTIATION; CARTILAGE; OSTEOGENESIS; CELLS; RATS; FGF AB In the growth plate, stem-like cells in the resting zone differentiate into rapidly dividing chondrocytes of the proliferative zone and then terminally differentiate into the nondividing chondrocytes of the hypertrophic zone. To explore the molecular switches responsible for this two-step differentiation program, we developed a microdissection method to isolate RNA from the resting (RZ), proliferative (PZ), and hypertrophic zones (HZ) of 7-day-old male rats. Expression of approximately 29 000 genes was analyzed by microarray and selected genes verified by real-time PCR. The analysis identified genes whose expression changed dramatically during the differentiation program, including multiple genes functionally related to bone morphogenetic proteins (BMPs). BMP-2 and BMP-6 were upregulated in HZ compared with RZ and PZ (30-fold each, P<0.01 and 0.001 respectively). In contrast, BMP signaling inhibitors were expressed early in the differentiation pathway; BMP-3 and gremlin were differentially expressed in RZ (100- and 80-fold, compared with PZ, P<0.001 and 0.005 respectively) and growth differentiation factor (GDF)-10 in PZ (160-fold compared with HZ, P<0.001). Our findings suggest a BMP signaling gradient across the growth plate, which is established by differential expression of multiple BMPs and BMP inhibitors in specific zones. Since BMPs can stimulate both proliferation and hypertrophic differentiation of growth plate chondrocytes, these findings, suggest that low levels of BMP signaling in the resting zone may help maintain these cells in a quiescent state. In the lower RZ, greater BMP signaling may help induce differentiation to proliferative chondrocytes. Farther down the growth plate, even greater BMP signaling may help induce hypertrophic differentiation. Thus, BMP signaling gradients may be a key mechanism responsible for spatial regulation of chondrocyte proliferation and differentiation in growth plate cartilage. C1 NICHHD, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA. RP Nilsson, O (reprint author), Karolinska Hosp, Astrid Lindgren Childrens Hosp, Pediat Endocrinol Unit, Q2-08, Stockholm, Sweden. EM ola.nilsson@ki.se OI Nilsson, Ola/0000-0002-9986-8138 FU Intramural NIH HHS NR 28 TC 51 Z9 54 U1 0 U2 0 PU SOC ENDOCRINOLOGY PI BRISTOL PA 22 APEX COURT, WOODLANDS, BRADLEY STOKE, BRISTOL BS32 4JT, ENGLAND SN 0022-0795 J9 J ENDOCRINOL JI J. Endocrinol. PD APR PY 2007 VL 193 IS 1 BP 75 EP 84 DI 10.1677/joe.1.07099 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 159WE UT WOS:000245897500009 PM 17400805 ER PT J AU Zadeh, KS Montas, HJ Shirmohammadi, A Sadeghzadeh, A Gudla, PR AF Zadeh, Kouroush Sadegh Montas, Hubert J. Shirmohammadi, Adel Sadeghzadeh, Ali Gudla, Prabhakar R. TI A diagnosis-prescription system for nitrogen management in environment SO JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING LA English DT Article DE decision support system; expert system; geographic information system (GIS); best management practices ID NITRATE REMOVAL; WATER-QUALITY; PHOSPHORUS; WETLAND AB A decision support system in the framework of the geographic information system (GIS) and subsurface flow model, Hydrosub, were used to identify critical areas from simulated spatial distributions of relative nitrogen export. Diagnosis and prescription Expert Systems (ES) are developed and applied to the identification of probable causes of excessive nitrogen export and selection of appropriate Best Management Practices (BMPs). The result is a spatially distributed set of recommended Best Management Practices that are feasible economically and environmentally. For the study watershed, using catch crops and rhizobium-legume (instead of using conventional commercial fertilizers) were the most recommended Best Management Practices. C1 Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA. NCI, SAIC, Imaging Anal Lab, Frederick, MD 21701 USA. Natl Iranian Oil Co, Tehran, Iran. RP Zadeh, KS (reprint author), Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA. EM kouroush@eng.umd.edu NR 17 TC 3 Z9 3 U1 1 U2 4 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 1093-4529 J9 J ENVIRON SCI HEAL A JI J. Environ. Sci. Health Part A-Toxic/Hazard. Subst. Environ. Eng. PD APR PY 2007 VL 42 IS 5 BP 557 EP 565 DI 10.1080/10934520701244003 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 157JJ UT WOS:000245715200002 ER PT J AU Paukner, A Anderson, JR Donaldson, DI Ferrari, PF AF Paukner, Annika Anderson, James R. Donaldson, David I. Ferrari, Pier F. TI Cued repetition of self-directed behaviors in macaques (Macaca nemestrina) SO JOURNAL OF EXPERIMENTAL PSYCHOLOGY-ANIMAL BEHAVIOR PROCESSES LA English DT Article DE pigtailed macaques; self-directed behavior; repeat signal; internal stimulus; episodic memory ID EPISODIC-LIKE MEMORY; SCRUB JAYS; MONKEYS; DISCRIMINATION; CONSCIOUSNESS; REMEMBER; MULATTA AB Two macaques (Macaca nemestrina) were trained to perform 3 self-directed behaviors on signal and to repeat behaviors after a 'repeat' signal. The cognitive processes underlying the monkeys' repeat performance were evaluated via multiple repetitions of the repeat signal, extended delay periods between target behavior and repeat signal, and by transferring the repeat signal to novel behaviors. The monkeys seemed to use representations of their own past behaviors as a basis for repetition performance, but they mostly failed to correctly repeat target behaviors after extended delays and during transfer tasks. Implications for episodic memory abilities are discussed. C1 NIH, Anim Ctr, Comparat Ethol Lab, Poolesville, MD 20837 USA. Univ Stirling, Dept Psychol, Stirling FK9 4LA, Scotland. Univ Parma, Dipartimento Neurosci, I-43100 Parma, Italy. RP Paukner, A (reprint author), NIH, Anim Ctr, Comparat Ethol Lab, POB 529, Poolesville, MD 20837 USA. EM pauknera@mail.nih.gov RI Donaldson, David/A-5249-2009 OI Donaldson, David/0000-0002-8036-3455 NR 28 TC 0 Z9 0 U1 1 U2 4 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 USA SN 0097-7403 J9 J EXP PSYCHOL ANIM B JI J. Exp. Psychol.-Anim. Behav. Process. PD APR PY 2007 VL 33 IS 2 BP 139 EP 147 DI 10.1037/0097-7403.33.2.139 PG 9 WC Psychology, Biological; Behavioral Sciences; Psychology; Psychology, Experimental; Zoology SC Psychology; Behavioral Sciences; Zoology GA 157EC UT WOS:000245700400006 PM 17469962 ER PT J AU De Soto, J AF De Soto, Joseph TI Should HPV vaccination be mandatory? SO JOURNAL OF FAMILY PRACTICE LA English DT Editorial Material C1 NIDDKD, Magnuson Clin Ctr, Mammalian Genet Genet Dis & Dev Branch, NIH, Bethesda, MD 20892 USA. RP De Soto, J (reprint author), NIDDKD, Magnuson Clin Ctr, Mammalian Genet Genet Dis & Dev Branch, NIH, Bethesda, MD 20892 USA. EM desotoj@niddk.nih.gov NR 0 TC 5 Z9 5 U1 0 U2 0 PU DOWDEN PUBLISHING CORP PI MONTVALE PA 110 SUMMIT AVE, MONTVALE, NJ 07645-1712 USA SN 0094-3509 J9 J FAM PRACTICE JI J. Fam. Pract. PD APR PY 2007 VL 56 IS 4 BP 267 EP 268 PG 2 WC Primary Health Care; Medicine, General & Internal SC General & Internal Medicine GA 158GM UT WOS:000245779700002 PM 17403324 ER PT J AU Mcdermott, MM Ferrucci, L Guralnik, JM Tian, L Green, D Liu, K Tan, J Pearce, WH Schneider, JR Ridker, P Rifar, N Hoff, FL Criqui, MH AF Mcdermott, M. M. Ferrucci, L. Guralnik, J. M. Tian, L. Green, D. Liu, K. Tan, J. Pearce, W. H. Schneider, J. R. Ridker, P. Rifar, N. Hoff, F. L. Criqui, M. H. TI Circulating blood markers and calf muscle characteristics in peripheral arterial disease SO JOURNAL OF GENERAL INTERNAL MEDICINE LA English DT Meeting Abstract CT 30th Annual Meeting of the Society-of-General-Internal-Medicine CY APR 25-28, 2007 CL Toronto, CANADA SP Soc Gen Internal Med C1 [Mcdermott, M. M.; Tian, L.; Green, D.; Liu, K.; Tan, J.; Pearce, W. H.; Hoff, F. L.] Northwestern Univ, Chicago, IL USA. [Ferrucci, L.; Guralnik, J. M.] NIA, Bethesda, MD USA. [Schneider, J. R.] Northwestern Univ, Med Ctr, Chicago, IL USA. [Ridker, P.; Rifar, N.; Hoff, F. L.] Univ Calif San Diego, La Jolla, CA 92093 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 0884-8734 J9 J GEN INTERN MED JI J. Gen. Intern. Med. PD APR PY 2007 VL 22 SU 1 BP 4 EP 4 PG 1 WC Health Care Sciences & Services; Medicine, General & Internal SC Health Care Sciences & Services; General & Internal Medicine GA 240TQ UT WOS:000251610700012 ER PT J AU Sueoka, K Goulet, J Butt, AA Crystal, S Gibert, CL Rimland, D Bryant, KJ Rodriguez-Barradas, M Justice, AC AF Sueoka, K. Goulet, J. Butt, A. A. Crystal, S. Gibert, C. L. Rimland, D. Bryant, K. J. Rodriguez-Barradas, M. Justice, A. C. TI Depression treatment among HIV+/- veterans: Does provider comfort predict treatment? SO JOURNAL OF GENERAL INTERNAL MEDICINE LA English DT Meeting Abstract CT 30th Annual Meeting of the Society-of-General-Internal-Medicine CY APR 25-28, 2007 CL Toronto, CANADA SP Soc Gen Internal Med C1 [Sueoka, K.] Yale Univ, New Haven, CT USA. [Goulet, J.; Justice, A. C.] Yale Univ, Vet Affairs Med Ctr, West Haven, CT USA. [Butt, A. A.] Univ Pittsburgh, Vet Affairs Med Ctr, Pittsburgh, PA USA. [Crystal, S.] SUNY New Brunswick, New Brunswick, NJ USA. [Rimland, D.] George Washington Univ, Med Ctr, Vet Affairs Med Ctr, Washington, DC USA. [Rimland, D.] Emory Univ, Vet Affairs Med Ctr, Decatur, GA USA. [Bryant, K. J.] NIAAA, NIH, Bethesda, MD USA. [Rodriguez-Barradas, M.] Baylor Coll Med, Vet Affairs Med Ctr, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 0884-8734 J9 J GEN INTERN MED JI J. Gen. Intern. Med. PD APR PY 2007 VL 22 SU 1 BP 17 EP 17 PG 1 WC Health Care Sciences & Services; Medicine, General & Internal SC Health Care Sciences & Services; General & Internal Medicine GA 240TQ UT WOS:000251610700059 ER PT J AU Wong, MD Ambs, AH Goldstein, MS Becerra, L Cheng, E Wenger, NS Hsiao, A AF Wong, M. D. Ambs, A. H. Goldstein, M. S. Becerra, L. Cheng, E. Wenger, N. S. Hsiao, A. TI The unexpected associations of religiosity and spirituality with complementary and alternative medicine use among cancer survivors SO JOURNAL OF GENERAL INTERNAL MEDICINE LA English DT Meeting Abstract CT 30th Annual Meeting of the Society-of-General-Internal-Medicine CY APR 25-28, 2007 CL Toronto, CANADA SP Soc Gen Internal Med C1 [Wong, M. D.; Goldstein, M. S.; Becerra, L.; Wenger, N. S.] Univ Calif Los Angeles, Los Angeles, CA USA. [Ambs, A. H.] NCI, Bethesda, MD USA. [Cheng, E.] Greater Los Angeles Vet Hlthcare Syst, Los Angeles, CA USA. [Hsiao, A.] Long Beach VA Hlthcare Syst, Long Beach, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 0884-8734 J9 J GEN INTERN MED JI J. Gen. Intern. Med. PD APR PY 2007 VL 22 SU 1 BP 33 EP 34 PG 2 WC Health Care Sciences & Services; Medicine, General & Internal SC Health Care Sciences & Services; General & Internal Medicine GA 240TQ UT WOS:000251610700115 ER PT J AU Frank, D Gallagher, TH Cooper, LA Odunlami, B Sellers, S Price, EG Phillips, E Bonham, VL AF Frank, D. Gallagher, T. H. Cooper, L. A. Odunlami, B. Sellers, S. Price, E. G. Phillips, E. Bonham, V. L. TI Physicians' attitudes regarding race-based therapeutics SO JOURNAL OF GENERAL INTERNAL MEDICINE LA English DT Meeting Abstract CT 30th Annual Meeting of the Society-of-General-Internal-Medicine CY APR 25-28, 2007 CL Toronto, CANADA SP Soc Gen Internal Med C1 [Frank, D.] VA Puget Sound, Seattle, WA USA. [Gallagher, T. H.] Univ Washington, Seattle, WA 98195 USA. [Cooper, L. A.] Johns Hopkins Univ, Baltimore, MD 21218 USA. [Odunlami, B.; Phillips, E.; Bonham, V. L.] Natl Inst Hlth, Bethesda, MD USA. [Sellers, S.] Univ Wisconsin, Madison, WI 53706 USA. [Price, E. G.] Tulane Univ, New Orleans, LA 70118 USA. RI Price-Haywood, Eboni/L-5527-2014 OI Price-Haywood, Eboni/0000-0003-2901-3852 NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 0884-8734 J9 J GEN INTERN MED JI J. Gen. Intern. Med. PD APR PY 2007 VL 22 SU 1 BP 155 EP 156 PG 2 WC Health Care Sciences & Services; Medicine, General & Internal SC Health Care Sciences & Services; General & Internal Medicine GA 240TQ UT WOS:000251610700540 ER PT J AU Kronman, AC Freund, KM Ash, A Emanuel, E AF Kronman, A. C. Freund, K. M. Ash, A. Emanuel, E. TI Primary care visits mediate gender differences in utilization of hospital services at the end of life SO JOURNAL OF GENERAL INTERNAL MEDICINE LA English DT Meeting Abstract CT 30th Annual Meeting of the Society-of-General-Internal-Medicine CY APR 25-28, 2007 CL Toronto, CANADA SP Soc Gen Internal Med C1 [Kronman, A. C.; Freund, K. M.; Ash, A.] Boston Univ, Boston, MA 02215 USA. [Emanuel, E.] Natl Inst Hlth, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 0884-8734 J9 J GEN INTERN MED JI J. Gen. Intern. Med. PD APR PY 2007 VL 22 SU 1 BP 169 EP 169 PG 1 WC Health Care Sciences & Services; Medicine, General & Internal SC Health Care Sciences & Services; General & Internal Medicine GA 240TQ UT WOS:000251610700587 ER PT J AU Sawiris, GP Becker, KG Elliott, EJ Moulden, R Rohwer, RG AF Sawiris, G. Peter Becker, Kevin G. Elliott, Ellen J. Moulden, Robert Rohwer, Robert G. TI Molecular analysis of bovine spongiform encephalopathy infection by cDNA arrays SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID DIFFERENTIALLY EXPRESSED GENES; CREUTZFELDT-JAKOB-DISEASE; UP-REGULATED GENES; CELL-DEATH; NEUROBLASTOMA-CELLS; ALZHEIMERS-DISEASE; PRION PROTEIN; BRAIN-TISSUE; MOUSE BRAINS; SCRAPIE AB Here, the first cDNA array analysis of differential gene expression in bovine spongiform encephalopathy (BSE) is reported, using a spotted cDNA array platform representing nearly 17 000 mouse genes. Array analysis identified 296 gene candidates for differential expression in brain tissue from VM mice in late-stage infection with the 301V strain of BSE, compared with brain tissue from normal, age-matched VM mice. Real-time PCR confirmed differential expression of 25 of 31 genes analysed. Some of the genes identified by array analysis as being expressed differentially are associated with ubiquitin/proteasome function, lysosomal function, molecular chaperoning of protein folding or apoptosis. Other genes are involved in calcium ion binding/homeostasis, zinc ion binding/homeostasis or regulation of transcription. Principal-component analysis shows that the global gene-expression profiles of the BSE-infected samples have gene-expression signatures that are markedly different from, and completely non-overlapping with, those obtained from the normal controls. C1 VA Maryland Healthcare Syst, Res Serv, Baltimore, MD USA. Natl Inst Aging, Gene Express & Genom Unit, Baltimore, MD USA. Univ Maryland, Sch Med, Dept Neurol, Baltimore, MD USA. RP Rohwer, RG (reprint author), VA Maryland Healthcare Syst, Res Serv, Baltimore, MD USA. EM rrohwer@umaryland.edu OI Becker, Kevin/0000-0002-6794-6656 FU NINDS NIH HHS [N01-NS-0-2327] NR 44 TC 19 Z9 20 U1 1 U2 2 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 J9 J GEN VIROL JI J. Gen. Virol. PD APR PY 2007 VL 88 BP 1356 EP 1362 DI 10.1099/vir.0.82387-0 PN 4 PG 7 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA 154FY UT WOS:000245493500033 PM 17374782 ER PT J AU Kremer-Tal, S Narla, G Chen, YB Hod, E DiFeo, A Yea, S Lee, JS Schwartz, M Thung, SN Fiel, IM Banck, M Zimran, E Thorgeirsson, SS Mazzaferro, V Bruix, J Martignetti, JA Llovet, JM Friedman, SL AF Kremer-Tal, Sigal Narla, Goutham Chen, Yingbei Hod, Eldad DiFeo, Analisa Yea, Steven Lee, Ju-Seog Schwartz, Myron Thung, Swan N. Fiel, Isabel M. Banck, Michaela Zimran, Eran Thorgeirsson, Snorri S. Mazzaferro, Vincenzo Bruix, Jordi Martignetti, John A. Llovet, Josep M. Friedman, Scott L. TI Downregulation of KLF6 is an early event in hepatocarcinogenesis, and stimulates proliferation while reducing differentiation SO JOURNAL OF HEPATOLOGY LA English DT Article DE hepatocellular carcinoma; alternative splicing; KLF6SV1; dysplasia; E-cadherin ID TUMOR-SUPPRESSOR GENE; KRUPPEL-LIKE FACTOR-6; HUMAN HEPATOCELLULAR-CARCINOMA; PROSTATE-CANCER; BETA-CATENIN; CYCLIN D1; CLINICAL CHARACTERISTICS; ALPHA-FETOPROTEIN; STEM-CELLS; E-CADHERIN AB Background/Aims: Hepatocellular carcinoma (HCC) has the most rapidly rising cancer incidence in the US and Europe. The KLF6 tumor suppressor is frequently inactivated in HCC by loss-of-heterozygosity (LOH) and/or mutation. Methods: Here we have analyzed 33 HBV- and 40 HCV-related HCCs for mRNA expression of wildtype KLF6 (wtKLF6) as well as the KLF6 variant 1 (SV1), a truncated, growth-promoting variant that antagonizes wtKLF6 function. The HCV-related tumors analyzed represented the full histologic spectrum from cirrhosis and dysplasia to metastatic cancer. Results: Expression of KLF6 mRNA is decreased in 73% of HBV-associated HCCs compared to matched surrounding tissue (ST), with reductions of similar to 80% in one-third of the patients. KLF6 mRNA expression is also reduced in dysplastic nodules from patients with HCV compared to cirrhotic livers (p < 0.005), with an additional, marked decrease in the very advanced, metastatic stage (p < 0.05). An increased ratio of KLF6SV1/wt KLF6 is present in a subset (6/33, 18%) of the HBV-related HCCs compared to matched ST. Reconstituting KLF6 in HepG2 cells by retroviral infection decreased proliferation and related markers including cyclin D1 and beta-catenin, increased cellular differentiation based on induction of albumin, E-cadherin, and decreased alpha fetoprotein. Conclusions: We conclude that reduced KLF6 expression is common in both HBV- and HCV-related HCCs and occurs at critical stages during cancer progression. Effects of KLF6 are attributable to regulation of genes controlling hepatocyte growth and differentiation. (c) 2006 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. C1 Mt Sinai Sch Med, Div Liver Dis, New York, NY USA. Mt Sinai Sch Med, Dept Med, New York, NY USA. Mt Sinai Sch Med, Dept Human Genet, New York, NY USA. NCI, Expt Carcinogenesis Lab, Canc Res Ctr, NIH, Bethesda, MD 20892 USA. Univ Texas, MD Anderson Canc Ctr, Dept Mol Therapeut, Houston, TX 77030 USA. Mt Sinai Sch Med, Dept Surg, New York, NY USA. Mt Sinai Sch Med, Dept Pathol, New York, NY USA. Mt Sinai Sch Med, Recanati Miller Transplantat Inst, New York, NY USA. Mt Sinai Sch Med, Dept Pediat, New York, NY USA. Mt Sinai Sch Med, Dept Oncol Sci, New York, NY USA. Ben Gurion Med Sch, Beer Sheva, Israel. Natl Canc Inst, I-20133 Milan, Italy. Hosp Clin Barcelona, IDIBAPS, BCLC Grp, Liver Unit, Barcelona, Spain. RP Friedman, SL (reprint author), Mt Sinai Sch Med, Div Liver Dis, New York, NY USA. EM Scott.Friedman@mssm.edu RI Llovet, Josep M /D-4340-2014; Mazzaferro, Vincenzo/C-2726-2017 OI Llovet, Josep M /0000-0003-0547-2667; Bruix, Jordi/0000-0002-9826-0753; Mazzaferro, Vincenzo/0000-0002-4013-8085 FU NIDDK NIH HHS [R01 DK037340, 1 K24 DK 60498-03, DK37340, DK56621, K08 DK068026, K24 DK060498, KO8DK068026-01A1, R01 DK056621, R56 DK056621, T32 DK 07792-01, T32 DK007792]; NIGMS NIH HHS [T32 GM062754] NR 43 TC 54 Z9 55 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2007 VL 46 IS 4 BP 645 EP 654 DI 10.1016/j.jhep.2006.10.012 PG 10 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 158MF UT WOS:000245794900019 PM 17196295 ER PT J AU Oliver, C Fujimura, A Souza, AMMSE de Castro, RO Siraganian, RP Jamur, MC AF Oliver, Constance Fujimura, Akira Silveira e Souza, Adriana Maria Mariano de Castro, Rodrigo Orlandini Siraganian, Reuben P. Jamur, Maria Celia TI Mast cell-specific gangliosides and Fc epsilon RI follow the same endocytic pathway from lipid rafts in RBL-2H3 cells SO JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY LA English DT Article DE mast cells; Fc epsilon RI; lipid rafts; endocytosis; gangliosides; electron microscopy ID BASOPHILIC LEUKEMIA-CELLS; MONOCLONAL-ANTIBODY AA4; GROWTH-FACTOR RECEPTOR; DETERGENT-RESISTANT MEMBRANES; SCANNING ELECTRON-MICROSCOPY; ALPHA-GALACTOSYL DERIVATIVES; DIGITAL IMAGE-ANALYSIS; AFFINITY IGE RECEPTOR; SIGNAL-TRANSDUCTION; PLASMA-MEMBRANE AB Recent studies have shown that, in mast cells, membrane microdomains rich in cholesterol and glycosphingolipids called lipid rafts play an important role in FcERI signaling. The present study demonstrates that, in RBL-2H3 cells following stimulation, the mast cell-specific gangliosides associated with Fc epsilon RI are internalized from lipid rafts along with the receptor. When the cells are labeled with iodinated antibodies against the gangliosides or against FcERI and the cell components are then fractionated on Percoll density gradients, in stimulated cells the gangliosides are internalized with the same kinetics as Fc epsilon RI and at 3 hr are present in the dense lysosome fraction. Using transmission electron microscopy, with antibody against the gangliosides conjugated to horseradish peroxiclase and antibody against Fc epsilon RI conjugated to colloidal gold, it was possible to demonstrate that the gangliosides and Fc epsilon RI are internalized in the same coated vesicles. At 5 min, the gangliosides and Fc epsilon RI can be identified in early endosomes and at 3 hr are found together in acid phosphatase-positive lysosomes. This study demonstrates that the mast cell-specific gangliosides are internalized from lipid rafts in the same vesicles and traffic intracellularly with the same kinetics as Fc epsilon RI. This study contains online supplemental material at http://www.jhc. org. Please visit this article online to view these materials. C1 Univ Sao Paulo, Dept Cell & Mol Biol & Pathogen Bioagents, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto, Brazil. Natl Inst Dent & Craniofacial Res, Receptors & Signal Transduct Sect, Oral Infect & Immun Branch, NIH, Bethesda, MD USA. Iwate Med Univ, Sch Dent, Dept Oral Anat 1, Morioka, Iwate 020, Japan. RP Oliver, C (reprint author), Univ Sao Paulo, Dept Cell & Mol Biol & Pathogen Bioagents, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto, Brazil. EM coliver@fmrp.usp.br RI Jamur, Maria Celia/L-5520-2016 OI Jamur, Maria Celia/0000-0001-7065-8543 NR 66 TC 15 Z9 15 U1 0 U2 1 PU HISTOCHEMICAL SOC INC PI SEATTLE PA UNIV WASHINGTON, DEPT BIOSTRUCTURE, BOX 357420, SEATTLE, WA 98195 USA SN 0022-1554 J9 J HISTOCHEM CYTOCHEM JI J. Histochem. Cytochem. PD APR PY 2007 VL 55 IS 4 BP 315 EP 325 DI 10.1369/jhc.6A7037.2006 PG 11 WC Cell Biology SC Cell Biology GA 151NW UT WOS:000245298400002 PM 17164410 ER PT J AU Sulek, J Wagenaar-Miller, RA Shireman, J Molinolo, A Madsen, DH Engelholm, LH Behrendt, N Bugge, TH AF Sulek, Jay Wagenaar-Miller, Rebecca A. Shireman, Jessica Molinolo, Alfredo Madsen, Daniel H. Engelholm, Lars H. Behrendt, Niels Bugge, Thomas H. TI Increased expression of the collagen internalization receptor uPARAP/Endo180 in the stroma of head and neck cancer SO JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY LA English DT Article DE collagen degradation; Endo180; head and neck cancer; invasion; squamous cell carcinoma; urokinase plasminogen activator receptor-associated protein ID SQUAMOUS-CELL CARCINOMA; PLASMINOGEN-ACTIVATOR RECEPTOR; UROKINASE RECEPTOR; EXTRACELLULAR-MATRIX; TURNOVER; ENDO180; PROTEIN; GENE; METALLOPROTEINASES; PROTEIN/ENDO180 AB Local growth, invasion, and metastasis of malignancies of the head and neck involve extensive degradation and remodeling of the underlying, collagen-rich connective tissue. Urokinase plasminogen activator receptor-associated protein (uPARAP)/Endo180 is an endocytic receptor recently shown to play a critical role in the uptake and intracellular degradation of collagen by mesenchymal cells. As a step toward determining the putative function of uPARAP/Endo180 in head and neck cancer progression, we used immunohistochemistry to determine the expression of this collagen internalization receptor in 112 human squamous cell carcinomas and 19 normal or tumor-adjacent head and neck tissue samples from the tongue, gingiva, cheek, tonsils, palate, floor of mouth, larynx, maxillary sinus, upper jaw, nasopharynx/nasal cavity, and lymph nodes. Specificity of detection was verified by staining of serial sections with two different monoclonal antibodies against two non-overlapping epitopes on uPARAP/Endo180 and by the use of isotype-matched non-immune antibodies. uPARAP/Endo180 expression was observed in stromal fibroblast-like, vimentin-positive cells. Furthermore, expression of the collagen internalization receptor was increased in tumor stroma compared with tumor-adjacent connective tissue or normal submucosal connective tissue and was most prominent in poorly differentiated tumors. These data suggest that uPARAP/Endol 80 participates in the connective tissue destruction during head and neck squamous cell carcinoma progression by mediating cellular uptake and lysosomal degradation of collagen. C1 Natl Inst Dent & Craniofacial Res, Proteases & Tissue Remodeling Unit, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD 20892 USA. Rigshosp, Finsen Lab, DK-2100 Copenhagen, Denmark. RP Bugge, TH (reprint author), Natl Inst Dent & Craniofacial Res, Proteases & Tissue Remodeling Unit, Oral & Pharyngeal Canc Branch, NIH, 30 Convent Dr,Room 211, Bethesda, MD 20892 USA. EM thomas.bugge@nih.gov OI Madsen, Daniel Hargboel/0000-0002-3183-6201; Engelholm, Lars/0000-0002-6616-1232 FU Intramural NIH HHS NR 33 TC 35 Z9 37 U1 0 U2 3 PU HISTOCHEMICAL SOC INC PI SEATTLE PA UNIV WASHINGTON, DEPT BIOSTRUCTURE, BOX 357420, SEATTLE, WA 98195 USA SN 0022-1554 J9 J HISTOCHEM CYTOCHEM JI J. Histochem. Cytochem. PD APR PY 2007 VL 55 IS 4 BP 347 EP 353 DI 10.1369/jhc.6A7133.2006 PG 7 WC Cell Biology SC Cell Biology GA 151NW UT WOS:000245298400005 PM 17189524 ER PT J AU Ogbureke, KUE Fisher, LW AF Ogbureke, Kalu U. E. Fisher, Larry W. TI SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity SO JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY LA English DT Article DE SIBLINGs; osteopontin; bone sialoprotein; dentin sialophosphoprotein; dentin matrix protein 1; matrix extracellular phosphoglycoprotein protein; normal duct epithelia; lacrimal gland; metabolically active duct ID HUMAN BREAST-CANCER; INTEGRIN-BINDING LIGAND; BONE SIALOPROTEIN; MATRIX METALLOPROTEINASES; PHOSPHORUS HOMEOSTASIS; SALIVARY-GLANDS; SWEAT GLANDS; DRY EYE; OSTEOPONTIN; PROTEINS AB The SIBLING (Small Integrin-Binding LIgand, N-linked Glycoprotein) family of secreted glycophosphoproteins includes bone sialoprotein (BSP), dentin matrix protein-1 (DMP1), dentin sialophosphoprotein (DSPP), osteopontin (OPN), and matrix extracellular phosphoglycoprotein (MEPE). For many years, they were thought in normal adults to essentially be limited to metabolically active mesenchymal cells that assembled the mineralized matrices of bones and teeth. Over the last decade they have also been upregulated in a variety of tumors. Three of these proteins (BSP, OPN, and DMP1) have been shown to interact with three matrix metalloproteinases (MMP-2, MMP-3, and MMP-9, respectively). Recently, all five SIBLINGs and their MMP partners when known were observed in specific elements of normal ductal epithelia in salivary gland and kidney. We have hypothesized that the SIBLINGs and their MMP partners may be expressed in ductal cells with high metabolic activity. In this we show that all the SIBLINGs (except MEPE) and their MMP partners are expressed in paper, the metabolically active epithelia of human eccrine sweat gland duct but not in the more passive ductal cells of the macaque (monkey) lacrimal gland. It is hypothesized that MEPE expression may be limited to cells involved in active phosphate transport. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. C1 Med Coll Georgia, Sch Dent, Dept Oral & Maxillofacial Pathol, Augusta, GA 30912 USA. Natl Inst Dent & Craniofacial Res, Matrix Biochem Unit, Craniofacial & Skeletal Dis Branch, NIH,Dept Hlth & Human Serv, Bethesda, MD USA. RP Ogbureke, KUE (reprint author), Med Coll Georgia, Sch Dent, Dept Oral & Maxillofacial Pathol, AD1442,1120 15th St, Augusta, GA 30912 USA. EM kogbureke@mail.mcg.edu FU Intramural NIH HHS NR 43 TC 53 Z9 57 U1 0 U2 0 PU HISTOCHEMICAL SOC INC PI SEATTLE PA UNIV WASHINGTON, DEPT BIOSTRUCTURE, BOX 357420, SEATTLE, WA 98195 USA SN 0022-1554 J9 J HISTOCHEM CYTOCHEM JI J. Histochem. Cytochem. PD APR PY 2007 VL 55 IS 4 BP 403 EP 409 DI 10.1369/jhc.6A7075.2007 PG 7 WC Cell Biology SC Cell Biology GA 151NW UT WOS:000245298400010 PM 17210923 ER PT J AU Davidson, TS DiPaolo, RJ Andersson, J Shevach, EM AF Davidson, Todd S. DiPaolo, Richard J. Andersson, John Shevach, Ethan M. TI Cutting edge: IL-2 is essential for TGF-beta-mediated induction of Foxp(3+) T regulatory cells SO JOURNAL OF IMMUNOLOGY LA English DT Article ID SUPPRESSOR FUNCTION; FOXP3 EXPRESSION; INTERLEUKIN-2; DISEASE AB TGF-beta is a pluripotent cytokine that is capable of inducing the expression of Foxp3 in naive T lymphocytes. TGF-beta-induced cells are phenotypically similar to thymic-derived regulatory T cells in that they are anergic and suppressive. We have examined the cytokine and costimulatory molecule requirements for TGF-beta-mediated induction and maintenance of Foxp3 by CD4(+)Foxp3(-) cells. IL-2 plays a non-redundant role in TGF-beta-induced Foxp3 expression. Other common gamma-chain-utilizing, cytokines were unable to induce Foxp3 expression in IL-2-deficient T cells. The role of CD28 in the induction of Foxp3 was solely related to its capacity to enhance the endogenous production of IL-2. Toxp3 expression was stable in vitro and in vivo in the absence of IL-2. As TGF-beta-induced T regulatory cells can be easily grown in vitro, they may prove useful for the treatment of autoimmune diseases, for the prevention of graft rejection, and graft versus host disease. C1 NIAID, Cellular Immunol Sect, Immunol Lab, NIH, Bethesda, MD 20892 USA. RP Shevach, EM (reprint author), NIAID, Cellular Immunol Sect, Immunol Lab, NIH, Bldg 10,11N315, Bethesda, MD 20892 USA. EM eshevach@niaid.nih.gov RI Andersson, John/A-4436-2009 NR 12 TC 281 Z9 299 U1 0 U2 14 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 IS 7 BP 4022 EP 4026 PG 5 WC Immunology SC Immunology GA 150EB UT WOS:000245197300005 PM 17371955 ER PT J AU Couper, KN Blount, DG de Souza, JB Suffia, I Belkaid, Y Riley, EM AF Couper, Kevin N. Blount, Daniel G. de Souza, J. Brian Suffia, Isabelle Belkaid, Yasmine Riley, Eleanor M. TI Incomplete depletion and rapid regeneration of Foxp3(+) regulatory T cells following anti-CD25 treatment in malaria-infected mice SO JOURNAL OF IMMUNOLOGY LA English DT Article ID TRANSCRIPTION FACTOR FOXP3; GENERATED EX-VIVO; TGF-BETA; IN-VIVO; DENDRITIC CELLS; CUTTING EDGE; FUNCTIONAL INACTIVATION; IMMUNOLOGICAL-TOLERANCE; AUTOIMMUNE-DISEASE; CD4(+) AB Investigation of the role of regulatory T cells (Treg) in model systems is facilitated by their depletion using anti-CD25 Abs, but there has been considerable debate about the effectiveness of this strategy. In this study, we have compared the depletion and repopulation of CD4(+)CD25(+)Foxp3(+) Treg in uninfected and malaria-infected mice using 7D4 and/or PC61 anti-CD25 Abs. We find that numbers and percentages of CD25(high) cells, but not Foxp3(+) cells, are transiently reduced after 7D4 treatment, whereas treatment with PC61 alone or in combination with 7D4 (7D4 plus PC61) reduces but does not eliminate Foxp3+ cells for up to 2 wk. Importantly, all protocols fail to eliminate significant populations of CD25(-)Foxp3(+) or CD25(low)Foxp3(+) cells, which retain potent regulatory capacity. By adoptive transfer we show that repopulation of the spleen by CD25high Foxp3(+) cells results from the re-expression of CD25 on peripheral populations of CD25(-)Foxp3(+) but not from the conversion of peripheral Foxp3(-) cells. CD25(high)Foxp3(+) repopulation occurs more rapidly in 7D4-treated mice than in 7D4 plus PC61-treated mice, reflecting ongoing clearance of emergent CD25(+)Foxp3(+) cells by persistent PC61 Ab. However, in 7D4 plus PC61-treated mice undergoing acute malaria infection, repopulation of the spleen by CD25(+)Foxp3(+) cells occurs extremely rapidly, with malaria infection driving proliferation and CD25 expression in peripheral CD4(+)CD25(-)Foxp3(-) cells and/or conversion of CD4(+)CD25(-)Foxp3(-) cells. Finally, we reveal an essential role for IL-2 for the re-expression of CD25 by Foxp3(+) cells after anti-CD25 treatment and observe that TGF-beta is required, in the absence of CD25 and IL-2, to maintain splenic Foxp3(+) cell numbers and a normal ratio of Treg:non-Treg cells. C1 Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, Immunol Unit, London WC1E 7HT, England. UCL, Sch Med, Dept Immunol & Mol Pathol, London W1N 8AA, England. NIAID, Mucosal Immunol Unit, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. RP Riley, EM (reprint author), Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, Immunol Unit, Keppel St, London WC1E 7HT, England. EM eleanoi.riley@lshtm.ac.uk RI Couper, Kevin/C-2419-2009; de Souza, Joseph/D-4979-2009; Riley, Eleanor/C-8960-2013; OI Riley, Eleanor/0000-0003-3447-3570; Couper, Kevin/0000-0003-4659-8960 FU Wellcome Trust [074538] NR 62 TC 105 Z9 108 U1 1 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 IS 7 BP 4136 EP 4146 PG 11 WC Immunology SC Immunology GA 150EB UT WOS:000245197300019 PM 17371969 ER PT J AU Jirmanova, L Jankovic, D Fornace, AJ Ashwell, JD AF Jirmanova, Ludmila Jankovic, Dragana Fornace, Albert J., Jr. Ashwell, Jonathan D. TI Gadd45 alpha regulates p38-dependent dendritic cell cytokine production and Th1 differentiation SO JOURNAL OF IMMUNOLOGY LA English DT Article ID TOXOPLASMA-GONDII INFECTION; PROTEIN-KINASE ACTIVATION; IFN-GAMMA PRODUCTION; T-CELL; SCHISTOSOMA-MANSONI; CUTTING EDGE; P38; PATHWAY; RESISTANCE; IL-12 AB Gadd45 alpha inhibits the activation of p38 by the T cell alternative pathway involving phosphorylation of p38 Tyr(323). Given that T cell p38 may play a role in Th1 development, the response to Th-skewing Ags was analyzed in Gadd45 alpha(-/-) mice. Despite constitutively increased p38 activity in Gadd45 alpha(-/-) T cells, the Th1 immune response to Toxoplasma gondii Ag (STAg), was' diminished. In contrast to T cells, dendritic cells (DC) lacked the alternative p38 activation pathway. Gadd45 alpha(-/-) DCs responded to STAg with low levels of MAP kinase cascade-dependent p38 activation, IL-12 production, and CD40 expression. Wild-type T cells transferred into Gadd45 alpha(-/-) recipients had a diminished Th1 response to STAg, whereas Gadd45 alpha(-/-) T cells transferred into wild-type hosts behaved normally. Therefore, Gadd45a has tissue-specific and opposing functions on p38 activity, and Gadd45 alpha-regulated p38 activation in DCs is a critical event in Th1 polarization in vivo. C1 NCI, Lab Immune Biol, NIH, Bethesda, MD 20892 USA. NIH, Parasit Dis Lab, Bethesda, MD 20892 USA. Georgetown Univ, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA. RP Ashwell, JD (reprint author), NCI, Lab Immune Biol, NIH, Bldg 37,Room 3002, Bethesda, MD 20892 USA. EM jda@pop.nci.nih.gov RI Fornace, Albert/A-7407-2008 OI Fornace, Albert/0000-0001-9695-085X FU Intramural NIH HHS NR 38 TC 14 Z9 16 U1 0 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 IS 7 BP 4153 EP 4158 PG 6 WC Immunology SC Immunology GA 150EB UT WOS:000245197300021 PM 17371971 ER PT J AU Chen, JC Ellison, FM Eckhaus, MA Smith, AL Keyvanfar, K Calado, RT Young, NS AF Chen, Jichun Ellison, Felicia M. Eckhaus, Michael A. Smith, Aleah L. Keyvanfar, Keyvan Calado, Rodrigo T. Young, Neal S. TI Minor antigen H60-mediated aplastic anemia is ameliorated by immunosuppression and the infusion of regulatory T cells SO JOURNAL OF IMMUNOLOGY LA English DT Article ID VERSUS-HOST-DISEASE; BONE-MARROW-TRANSPLANTATION; HISTOCOMPATIBILITY ANTIGEN; IN-VIVO; MOUSE MODEL; AUTOIMMUNE-DISEASES; INTERFERON-GAMMA; CD4(+); RESPONSES; H60 AB Human bone marrow (BM) failure mediated by the immune system can be modeled in mice. In the present study, infusion of lymph node (LN) cells from C57BL/6 mice into C.B10-H2(b)/LilMed (C.B10) recipients that are mismatched at multiple minor histocompatibility Ags, including the immunodominant Ag H60, produced fatal aplastic anemia. Declining blood counts correlated with marked expansion and activation of CD8 T cells specific for the immunodominant minor histocompatibility Ag H60. Infusion of LN cells from 1160-matched donors did not produce BM failure in C.B10 mice, whereas isolated H60-specific CTL were cytotoxic for normal C.B10 BM cells in vitro. Treatment with the immunosuppressive drug cyclosporine abolished H60-specific T cell expansion and rescued animals from fatal pancytopenia. The development of BM failure was associated with a significant increase in activated CD4(+)CD25(+) T cells that did not express intracellular FoxP3, whereas inclusion of normal CD4(+)CD25(+) regulatory T cells in combination with C57BL/6 LN cells aborted H60-specific T cell expansion and prevented BM destruction. Thus, a single minor histocompatibility Ag H60 mismatch can trigger an immune response leading to massive BM destruction. Immunosuppressive drug treatment or enhancement of regulatory T cell function abrogated this pathophysiology and protected animals from the development of BM failure. C1 NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA. NIH, Div Vet Resources, Off Res Serv, Bethesda, MD 20892 USA. RP Chen, JC (reprint author), NHLBI, Hematol Branch, NIH, Bldg 10,Clin Res Ctr Rm 3-5132,10 Ctr Dr, Bethesda, MD 20892 USA. EM chenji@nhlbi.nih.gov RI Calado, Rodrigo/G-2619-2011 NR 47 TC 38 Z9 45 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 IS 7 BP 4159 EP 4168 PG 10 WC Immunology SC Immunology GA 150EB UT WOS:000245197300022 PM 17371972 ER PT J AU Bosio, CM Bielefeldt-Ohmann, H Belisle, JT AF Bosio, Catharine M. Bielefeldt-Ohmann, Helle Belisle, John T. TI Active suppression of the pulmonary immune response by Francisella tularensis Schu4 SO JOURNAL OF IMMUNOLOGY LA English DT Article ID LIVE VACCINE STRAIN; DENDRITIC CELLS; MURINE MACROPHAGES; TGF-BETA; MYCOBACTERIUM-TUBERCULOSIS; INTRACELLULAR BACTERIUM; INFECTION; ACTIVATION; EBOLA; MICE AB Francisella tularensis is an obligate, intracellular bacterium that causes acute, lethal disease following inhalation. As an intracellular,pathogen F. tularensis must invade cells, replicate, and disseminate while evading host immune responses. The mechanisms by which virulent type A strains of Francisella tularensis accomplish this evasion are not understood. Francisella tularensis has been shown to target multiple cell types in the lung following aerosol infection, including dendritic cells (DC) and macrophages. We demonstrate here that one mechanism used by a virulent type A strain of F. tularensis (Schu4) to evade early detection is by the induction of overwhelming immunosuppression at the site of infection, the lung. Following infection and replication in multiple pulmonary cell types, Schu4 failed to induce the production of proinflammatory cytokines or increase the expression of MHCH or CD86 on the surface of resident DC within the first few days of disease. However, Schu4 did induce early and transient production of TGF-beta, a potent immunosuppressive cytokine. The absence of DC activation following infection could not be attributed to the apoptosis of pulmonary cells, because there were minimal differences in either annexin or cleaved caspase-3 staining in infected mice compared with that in uninfected controls. Rather, we demonstrate that Schu4 actively suppressed in vivo responses to secondary stimuli (LPS), e.g., failure to recruit granulocytes/monocytes and stimulate resident DC. Thus, unlike attenuated strains of F. tularensis, Schu4 induced broad immunosuppression within the first few days after aerosol infection. This difference may explain the increased virulence of type A strains compared with their more attenuated counterparts. C1 Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. RP Bosio, CM (reprint author), NIAID, NIH, Rocky Mt Labs, 902 S 4th St, Hamilton, MT 59840 USA. EM bosioc@niaid.nih.gov RI Bielefeldt-Ohmann, Helle/A-3686-2010; Bosio, Catharine/D-7456-2015; Belisle, John/B-8944-2017 OI Belisle, John/0000-0002-2539-2798 FU NIAID NIH HHS [U01 AI 056487-01] NR 43 TC 131 Z9 131 U1 0 U2 3 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 IS 7 BP 4538 EP 4547 PG 10 WC Immunology SC Immunology GA 150EB UT WOS:000245197300062 PM 17372012 ER PT J AU Preller, V Gerber, A Wrenger, S Togni, M Marguet, D Tadje, J Lendeckel, U Rocken, C Faust, J Neubert, K Schraven, B Martin, R Ansorge, S Brocke, S Reinhold, D AF Preller, Vera Gerber, Annegret Wrenger, Sabine Togni, Mauro Marguet, Didier Tadje, Janine Lendeckel, Uwe Roecken, Christoph Faust, Juergen Neubert, Klaus Schraven, Burkhart Martin, Roland Ansorge, Siegfried Brocke, Stefan Reinhold, Dirk TI TGF-beta 1-mediated control of central nervous system inflammation and autoimmunity through the inhibitory receptor CD26 SO JOURNAL OF IMMUNOLOGY LA English DT Article ID DIPEPTIDYL-PEPTIDASE-IV; T-CELL-ACTIVATION; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; MULTIPLE-SCLEROSIS; TGF-BETA; IN-VIVO; DISEASE-ACTIVITY; DOWN-REGULATION; ORAL TOLERANCE; SUPPRESSION AB The T cell marker CD26/dipeptidyl peptidase (DP) IV is associated with an effector phenotype and markedly elevated in the human CNS disorder multiple sclerosis. However, little is known about the in vivo role of CD26/DP IV in health and disease, and the underlying mechanism of its function in CNS inflammation. To directly address the role of CD26/DP IV in vivo, we examined Th1 immune responses and susceptibility to experimental autoimmune encephalomyelitis in CD26(-/-) mice. We show that gene deletion of CD26 in mice leads to deregulation of Th1 immune responses. Although production of IFN-gamma and TNF-alpha by pathogenic T cells in response to myelin Ag was enhanced in CD26(-/-) mice, production of the immunosuppressive cytokine TGF-beta 1 was diminished in vivo and in vitro. In contrast to the reduction in TGF-beta 1 production, responsiveness to external TGF-beta 1 was normal in T cells from CD26(-/-) mice, excluding alterations in TGF-beta 1 sensitivity as a mechanism causing the loss of immune regulation. Natural ligands of CD26/DP IV induced TGF-beta 1 production in T cells from wild-type mice. However, natural ligands of CD26/DP IV failed to elicit TGF-beta 1 production in T cells from CD26(-/-) mice. The striking functional deregulation of Th1 immunity was also seen in vivo. Thus, clinical experimental autoimmune encephalomyelitis scores were significantly increased in CD26(-/-) mice immunized with peptide from myelin oligodendrocyte glycoprotein. These results identify CD26/DP IV as a nonredundant inhibitory receptor controlling T cell activation and Th1-mediated autoimmunity, and may have important therapeutic implications for the treatment of autoimmune CNS disease. C1 Otto Von Guericke Univ, Inst Immunol, D-39120 Magdeburg, Germany. Ctr Immunol Marseille Luminy, Marseille, France. Otto Von Guericke Univ, Inst Expt Internal Med, D-39120 Magdeburg, Germany. Otto Von Guericke Univ, Inst Pathol, D-39120 Magdeburg, Germany. Univ Halle Wittenberg, Inst Biochem, Dept Biochem & Biotechnol, Halle, Germany. NINDS, Cellular Immunol Sect, Neuroimmunol Branch, NIH, Bethesda, MD 20892 USA. IMTH GmbH, Magdeburg, Germany. Hebrew Univ Jerusalem, Dept Pathol, IL-91905 Jerusalem, Israel. Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06030 USA. RP Reinhold, D (reprint author), Otto Von Guericke Univ, Inst Immunol, Leipziger Str 44, D-39120 Magdeburg, Germany. EM dirk.reinhold@medizin.uni-magdeburg.de RI Marguet, Didier/B-9946-2008; Rocken, Christoph/A-9239-2010 OI Rocken, Christoph/0000-0002-6989-8002 NR 52 TC 46 Z9 46 U1 0 U2 6 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 IS 7 BP 4632 EP 4640 PG 9 WC Immunology SC Immunology GA 150EB UT WOS:000245197300072 PM 17372022 ER PT J AU Ahlenstiel, G Martin, MP Gao, XJ Carrington, M Rehermann, B AF Ahlenstiel, Golo Martin, Maureen P. Gao, Xiaojiang Carrington, Mary Rehermann, Barbara TI lKIR/HLA Compound Genotypes Determine Early Antiviral Response of Natural Killer Cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Ahlenstiel, Golo; Rehermann, Barbara] NIDDK, Immunol Sect, LDB, NIH,DHHS, Bethesda, MD 20892 USA. [Martin, Maureen P.; Gao, Xiaojiang; Carrington, Mary] NCI Frederick, Lab Genom Divers, SAIC Frederick Inc, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 35.17 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201053 ER PT J AU Anderson, SK Pascal, V Martin, MP Carrington, M Li, HC AF Anderson, Stephen K. Pascal, Veronique Martin, Maureen P. Carrington, Mary Li, Hongchuan TI Genetic control of variegated KIR gene expression: polymorphisms of the bi-directional KIR3DL1 promoter are associated with distinct frequencies of gene expression SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Anderson, Stephen K.; Pascal, Veronique] NCI Frederick, Lab Expt Immunol, Frederick, MD 21702 USA. [Anderson, Stephen K.; Li, Hongchuan] SAIC Frederick Inc, Basic Res Program, Frederick, MD 21702 USA. [Martin, Maureen P.; Carrington, Mary] NCI Frederick, Lab Genom Divers, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 35.37 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201039 ER PT J AU Baatar, D Olkhanud, P Almanzar, G Wells, V Mallucci, L Biragyn, A AF Baatar, DoIgor Olkhanud, Purevdorj Almanzar, Giovanni Wells, Valerie Mallucci, Livio Biragyn, Arya TI CD25(+)CD4(+) T regulatory cells utilize beta-galactoside-binding protein to suppress proliferation of CD8+T cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Baatar, DoIgor; Olkhanud, Purevdorj; Almanzar, Giovanni; Biragyn, Arya] NIA, Lab Immunol, Baltimore, MD 21224 USA. [Wells, Valerie; Mallucci, Livio] Kings Coll London, Sch Hlth & Life Sci, Cell Signaling & Growth Lab, London, England. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 88.40 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202082 ER PT J AU Bansal, G Xie, ZH Druey, K AF Bansal, Geetanjali Xie, Zhihui Druey, Kirk TI Rgs13 inhibits IgE-mediated allergic responses SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Bansal, Geetanjali; Xie, Zhihui; Druey, Kirk] NIAID, Lab Allerg Dis, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 37.5 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202238 ER PT J AU Bhatia, S Almo, SC Nathenson, SG Hodes, RJ AF Bhatia, Sumeena Almo, Steven C. Nathenson, Stanley G. Hodes, Richard J. TI The dynamic equilibrium between dimeric and monomeric cell surface B7-1 regulates T cell activation and CD28 expression SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Bhatia, Sumeena; Hodes, Richard J.] NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA. [Almo, Steven C.; Nathenson, Stanley G.] Albert Einstein Coll Med, Bronx, NY 10461 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 88.1 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202098 ER PT J AU Biragyn, A Olkhanud, PB Sumitomo, K Baatar, D AF Biragyn, Arya Olkhanud, Purevdorj B. Sumitomo, Kenya Baatar, DoIgor TI Depletion of CD4+CCR4+cells enhances T cell-mediated responses in vitro and vaccine-induced anti-tumor immune responses in mice SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Biragyn, Arya; Olkhanud, Purevdorj B.; Sumitomo, Kenya; Baatar, DoIgor] NIA, Immunotherapeut Unit, Immunol Lab, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 50.27 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202340 ER PT J AU Borrego, F Alvarez, Y Tang, XB Coligan, JE AF Borrego, Francisco Alvarez, Yelina Tang, Xiaobin Coligan, John E. TI Characterization of the inhibitory receptor CD300a in human neutrophils SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Borrego, Francisco; Alvarez, Yelina; Tang, Xiaobin; Coligan, John E.] NIAID, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 89.10 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203014 ER PT J AU Burgess, S Marusina, A Borrego, F Coligan, J AF Burgess, Steven Marusina, Alina Borrego, Francisco Coligan, John TI Identification and characterization of a conserved negative regulatory element in the human DAP10 gene SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Burgess, Steven; Marusina, Alina; Borrego, Francisco; Coligan, John] NIAID, RCBS, Rockville, MD 20850 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 35.27 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201054 ER PT J AU Butts, CL Belyayskaya, E Sternberg, EM AF Butts, Cherie L. Belyayskaya, Elena Sternberg, Esther M. TI Dendritic Cells from Male and Female Rodents Respond Differentially to Progesterone SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Butts, Cherie L.; Belyayskaya, Elena] NIH, Sect Neuroendocrine Immunol & Behav, Bethesda, MD 20892 USA. [Sternberg, Esther M.] NIMH, Sect Neuroendocrine Immunol & Behav, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 90.3 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203123 ER PT J AU Buzas, K Oppenheim, JJ Howard, OMZ AF Buzas, Krisztina Oppenheim, Joost J. Howard, O. M. Zack TI Two Chemotactic, Melanoma-Associated Proteins Stimulate Dendritic Cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Buzas, Krisztina; Oppenheim, Joost J.; Howard, O. M. Zack] NCI, Mol Immunoregulat Lab, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 48.3 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203295 ER PT J AU Caspi, R Silver, P Cua, D Chen, Z Chan, CC Luger, D AF Caspi, Rachel Silver, Phyllis Cua, Daniel Chen, Zoe Chan, Chi-Chao Luger, Dror TI IL-23 and IL-17 in pathogenesis of experimental ocular autoimmunity: requirement for IL-23 may extend beyond its role in sustaining the IL-17 effector response SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Caspi, Rachel; Silver, Phyllis; Chan, Chi-Chao; Luger, Dror] NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. [Cua, Daniel; Chen, Zoe] Schering Plough Inc, DNAX, Palo Alto, CA 94304 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 129.26 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201255 ER PT J AU Caucheteux, SM Younes, SA Paul, WE AF Caucheteux, Stephan M. Younes, Souheil A. Paul, William E. TI CD45RB expression refines delineation of memory CD4 T cells and aids in understanding their development SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Caucheteux, Stephan M.; Younes, Souheil A.; Paul, William E.] NIAID, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 91.8 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200348 ER PT J AU Chattopadhyay, G Khan, AQ Dubois, W Potter, M Snapper, CM AF Chattopadhyay, Gouri Khan, Abdul Q. Dubois, Wendy Potter, Michael Snapper, Clifford M. TI Transgenic expression of BcI-xL or BcI-2 by murine B cells enhances the in vivo anti-polysaccharide, but not anti-protein, response to Streptococcus pneumoniae SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Chattopadhyay, Gouri; Snapper, Clifford M.] Uniformed Serv Univ Hlth Sci, Pathol, Bethesda, MD 20814 USA. [Khan, Abdul Q.] Univ Maryland, Ctr Vaccine Dev, Sch Med, Baltimore, MD 21201 USA. [Dubois, Wendy; Potter, Michael] NIH, Genet Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 43.24 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200218 ER PT J AU Chaturvedi, A Pierce, S AF Chaturvedi, Akanksha Pierce, Susan TI B cell receptor signaling governs the subcellular location of Toll-like receptor 9 SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Chaturvedi, Akanksha; Pierce, Susan] NIAID, LIG, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 86.11 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203039 ER PT J AU Chiang, JY Jang, IK Hodes, R Gu, H AF Chiang, Jeffrey Y. Jang, Ihn Kyung Hodes, Richard Gu, Hua TI Ablation of Cbl-b Provides Protection Against Transplanted and Spontaneous Tumors SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Chiang, Jeffrey Y.; Hodes, Richard] NCI, EIB, NIH, Bethesda, MD 20892 USA. [Jang, Ihn Kyung; Gu, Hua] Columbia Univ, Dept Microbiol, Coll Phys & Surg, New York, NY 10032 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA B146 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200129 ER PT J AU Cox, CA Shi, G Yin, H Vistica, BP Wawrousek, EF Chan, CC Gery, I AF Cox, Catherine A. Shi, G. Yin, H. Vistica, B. P. Wawrousek, E. F. Chan, C-C. Gery, I. TI Polarized TCR-Transgenic Th17 Cells Resemble Th1 Cells in Their Capacity to Adoptively Transfer Ocular Inflammation, but Differ in Other Biological Activities SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Cox, Catherine A.; Shi, G.; Yin, H.; Vistica, B. P.; Wawrousek, E. F.; Chan, C-C.; Gery, I.] NEI, NIH, Bethesda, MD 20892 USA. [Cox, Catherine A.] NIH, HHMI, Res Scholar Program, Bethesda, MD 20814 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 130.44 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200031 ER PT J AU Crotty, S Moutaftsil, M McCausland, M Davies, DH Quiroz, JM Johnston, R Benhnia, RM Grey, H Hoffmann, J Head, S Garboczi, D Sette, A AF Crotty, Shane Moutaftsil, Magdalini McCausland, Megan Davies, D. Huw Quiroz, Juan Moyron Johnston, Robert Benhnia, Rafii Mohammed Grey, Howard Hoffmann, Julia Head, Steven Garboczi, David Sette, Alessandro TI Deterministic linkage between CD4 T cell and B cell specificities to a large virus: vaccinia virus SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Crotty, Shane; Moutaftsil, Magdalini; McCausland, Megan; Quiroz, Juan Moyron; Johnston, Robert; Benhnia, Rafii Mohammed; Grey, Howard; Sette, Alessandro] La Jolla Inst Allergy & Immunol LIAI, Vaccine Discovery, La Jolla, CA 92037 USA. [Davies, D. Huw] UC Irvine, Ctr Virus Res, Irvine, CA 92697 USA. [Hoffmann, Julia; Head, Steven] Scripps Res Insititute, Microarray Core, La Jolla, CA 92037 USA. [Garboczi, David] NIH Twinbrook, Lab Immunogenet LIG, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 43.1 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200183 ER PT J AU Dabitao, D Guindo, O Diallo, H Kassambara, H Washington, J Diallo, S Dao, S Tounkara, A Sereti, I Ciccone, E Catalfamo, M Lane, HC Siddiqui, S AF Dabitao, Djeneba Guindo, O. Diallo, H. Kassambara, H. Washington, J. Diallo, S. Dao, S. Tounkara, A. Sereti, I. Ciccone, E. Catalfamo, M. Lane, H. C. Siddiqui, S. TI Reconstitution of immune responses occurs very rapidly after initiation of therapy for tuberculosis SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Dabitao, Djeneba; Guindo, O.; Diallo, H.; Kassambara, H.; Diallo, S.; Dao, S.; Tounkara, A.] Univ Bamako, Immunol, Bamako, Mali. [Washington, J.; Sereti, I.; Ciccone, E.; Catalfamo, M.; Lane, H. C.; Siddiqui, S.] NIAID, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 43.54 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200199 ER PT J AU Das, A Long, E AF Das, Asmita Long, Eric TI Interplay of Activating and Inhibitory Receptors in NK cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Das, Asmita; Long, Eric] NIAID, LIG, NIH, Lab 207, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 89.28 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203022 ER PT J AU Deng, GM Lenardo, M AF Deng, Guo-Min Lenardo, Michael TI Pre-Ligand Assembly Domain (PLAD) is an important therapeutic target in inflammatory arthritis SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Deng, Guo-Min] Harvard Med Sch, Rheumatol, Beth Israel Deaconess Med Ctr, Boston, MA 02115 USA. [Lenardo, Michael] NIAID, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 131.21 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201302 ER PT J AU DiPaolo, RJ Davidson, TS Andersson, J Brinster, C Shevach, EM AF DiPaolo, Richard J. Davidson, Todd S. Andersson, John Brinster, Carine Shevach, Ethan M. TI Induced Organ-Specific T Regulatory (Treg) Cells Prevent Autoimmunity by Reducing the Ability of Dendritic Cells (DC) to Present Self-Antigen. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [DiPaolo, Richard J.; Davidson, Todd S.; Andersson, John; Brinster, Carine; Shevach, Ethan M.] NIAID, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 131.4 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201311 ER PT J AU Duyerger, A Tang, WJ Leppla, SH Boyaka, PN AF Duyerger, Alexandra Tang, Wei-Jen Leppla, Stephen H. Boyaka, Prosper N. TI Differential regulation of mucosal immunity by Bacillus anthracis edema toxin and cholera toxin as adjuvants for transcutaneous vaccines SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Duyerger, Alexandra; Boyaka, Prosper N.] Ohio State Univ, Vet Biosci, Columbus, OH 43210 USA. [Tang, Wei-Jen] Univ Chicago, Ben May Inst Canc Res, Chicago, IL 60637 USA. [Leppla, Stephen H.] NIAID, Microbial Pathogenesis Sect, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 41.3 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203127 ER PT J AU Dzutsev, A Belyakov, I Isakov, D Margulies, D Berzofsky, J AF Dzutsev, Amiran Belyakov, Igor Isakov, Dmitry Margulies, David Berzofsky, Jay TI Avidity of CD8 T-cells sharpens immunodominance. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Dzutsev, Amiran; Belyakov, Igor; Isakov, Dmitry; Berzofsky, Jay] NCI, Vaccine Branch, CCR, NIH, Bethesda, MD 20892 USA. [Margulies, David] NIAID, Lab Immunol, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA B9 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201202 ER PT J AU Farber, JM Song, KM Zhang, HWH Rabin, RL Hill, BJ Sereti, I Prussin, C Siegel, RM Douek, DC Roederer, M AF Farber, Joshua M. Song, Kaimei Zhang, Hongwei H. Rabin, Ronald L. Hill, Brenna J. Sereti, Irini Prussin, Colman Siegel, Richard M. Douek, Daniel C. Roederer, Mario TI CCR2 identifies first responders among human CD4+memory T cells: long-lived, apoptosis-resistant, antigen-responsive cells with enhanced migration potential, a low threshold for activation, and immediate effector capabilities SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Farber, Joshua M.; Song, Kaimei; Zhang, Hongwei H.; Hill, Brenna J.; Sereti, Irini; Prussin, Colman; Douek, Daniel C.; Roederer, Mario] NIAID, Bethesda, MD USA. [Rabin, Ronald L.] US FDA, Bethesda, MD USA. [Siegel, Richard M.] NIAMS, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 85.5 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202145 ER PT J AU George, T Harmon, I Peterson, EJ Burbach, BJ Shimizu, Y Matsuda, JL Gapin, L Connors, M Dowdell, KC Farrar, JD Hall, BE Morrissey, PJ AF George, Thaddeus Harmon, Ian Peterson, Erik J. Burbach, Brandon J. Shimizu, Yoji Matsuda, Jennifer L. Gapin, Laurent Connors, Mark Dowdell, Kennichi C. Farrar, J. David Hall, Brian E. Morrissey, Philip J. TI Measurement of nuclear translocation in primary cells using correlation analysis of images obtained on the ImageStream imaging flow cytometer SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [George, Thaddeus; Hall, Brian E.; Morrissey, Philip J.] Amnis Corp, Seattle, WA 98121 USA. [Harmon, Ian; Peterson, Erik J.; Burbach, Brandon J.; Shimizu, Yoji] Univ Minnesota, Sch Med, Ctr Immunol, Minneapolis, MN 55455 USA. [Matsuda, Jennifer L.; Gapin, Laurent] Natl Jewish Med & Res Ctr, Integrated Dept Immunol, Denver, CO 80206 USA. [Connors, Mark; Dowdell, Kennichi C.] NIAID, Immunoregulat Lab, Bethesda, MD 20892 USA. [Farrar, J. David] Univ Texas Southwestern Med Ctr, Ctr Immunol, Dallas, TX 75390 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 87.13 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203087 ER PT J AU Ghosh, MC Collins, G Carter, A Taub, DD AF Ghosh, Manik C. Collins, Gary Carter, Arnell Taub, Dennis D. TI Glucocorticoids Augment CXCR4-Mediated Signaling and Function Via the Activation of the Src Kinase, Lck SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Ghosh, Manik C.; Collins, Gary; Carter, Arnell; Taub, Dennis D.] NIA, Immunol Lab, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 94.10 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202223 ER PT J AU Gross, CC Long, EO AF Gross, Catharina Christiane Long, Eric O. TI The Role of Membrane Micro-domains on Target Cells in Control on NK Cell Activity SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Gross, Catharina Christiane; Long, Eric O.] NIAID, LIG, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 89.29 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203002 ER PT J AU Hall, VL Subleski, J Back, TC Gruys, ME Shorts-Cary, L Weiss, JM Wiltrout, RH AF Hall, Veronica L. Subleski, Jeff Back, Tim C. Gruys, M. Eilene Shorts-Cary, Lynnette Weiss, Jonathan M. Wiltrout, Robert H. TI Friend or Foe? IFN gamma Mediates Pro-Metastatic Gene Expression in the Tumor Microenvironment SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Hall, Veronica L.; Subleski, Jeff; Back, Tim C.; Gruys, M. Eilene; Weiss, Jonathan M.; Wiltrout, Robert H.] NCI, Expt Immunol Lab, Frederick, MD 21702 USA. [Shorts-Cary, Lynnette] Univ Colorado, Med Ctr, Denver, CO 80220 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 48.34 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203276 ER PT J AU Hesse, M Wilson, MS Cheever, AW Wynn, TA Bajracharya, S AF Hesse, Matthias Wilson, Mark S. Cheever, Allen W. Wynn, Thomas A. Bajracharya, Siddhartha TI CD103-expressing CD4+Foxp3+regulatory T cells are critical for the survival of mice infected with the helminth parasite Schistosoma mansoni. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Hesse, Matthias; Bajracharya, Siddhartha] Cornell Univ, Vet Coll, Immunol & Microbiol, Ithaca, NY 14853 USA. [Wilson, Mark S.; Wynn, Thomas A.] NIAID, Lab Parasit Dis, NIH, Bethesda, MD 20892 USA. [Cheever, Allen W.] Biomed Res Inst, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 46.17 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201213 ER PT J AU Hodges, MG Smith, A Urban, J Keane-Myers, A AF Hodges, Marcus Gomez Smith, Allen Urban, Joseph, Jr. Keane-Myers, Andrea TI The Immunomodulatory Effects of Ascaris suum Pseudocoelomic Fluid in Th1-Mediated Systems SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Hodges, Marcus Gomez; Keane-Myers, Andrea] NIAID, Allerg Inflammat Sect, Lab Allerg Dis, NIH,Twinbrook Facil 2, Rockville, MD 20852 USA. [Smith, Allen; Urban, Joseph, Jr.] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 90.10 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203119 ER PT J AU Horai, R Handon, R Broussard, C Mueller, K Venegas, AM Fowlkes, BJ Schwartzberg, PL AF Horai, Reiko Handon, Robin Broussard, Christine Mueller, Kristen Venegas, Ana M. Fowlkes, B. J. Schwartzberg, Pamela L. TI The Tec kinase Itk regulates conventional versus non-conventional CD8 T cell development SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Horai, Reiko; Handon, Robin; Broussard, Christine; Mueller, Kristen; Venegas, Ana M.; Schwartzberg, Pamela L.] NHGRI, Genet Dis Res Branch, NIH, Bethesda, MD 20892 USA. [Horai, Reiko] NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. [Fowlkes, B. J.] NIAID, Lab Cellular & Mol Immunol, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 87.14 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203103 ER PT J AU Howard, OMZ Buzas, K Dong, HF Oppenheim, JJ AF Howard, O. M. Zack Buzas, Krisztina Dong, Huifang Oppenheim, Joost J. TI Gp100 is Chemotactic for Mononuclear Cells and Utilizes Chemokine Receptors SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Howard, O. M. Zack; Buzas, Krisztina; Oppenheim, Joost J.] NCI, LMI, CIP, Frederick, MD 21702 USA. [Dong, Huifang] SAIC Frederick, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 96.4 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200276 ER PT J AU Hu, NJ Shikuma, C Shiramizu, B Koo, R Yewdell, JW AF Hu, Ningjie Shikuma, Cecilia Shiramizu, Bruce Koo, Raymond Yewdell, Jonathan W. TI Signaling pathways involved in foreign antigen expression by a recombinant vaccinia virus SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Hu, Ningjie; Shikuma, Cecilia; Shiramizu, Bruce; Koo, Raymond] Univ Hawaii Manoa, Dept Med, Leahi Hosp, Honolulu, HI 96816 USA. [Yewdell, Jonathan W.] NIAID, NIH, Viral Dis Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 89.38 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203010 ER PT J AU Imamichi, H Sereti, I Lane, HC AF Imamichi, Hiromi Sereti, Irini Lane, H. Clifford TI IL-15 acts as a potent inducer of CD4+CD25high cells expressing forkhead box protein P3 SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Imamichi, Hiromi] SAIC Frederick Inc, Clin Serv Program, Frederick, MD 21702 USA. [Sereti, Irini] NIAID, Lab Immunoregulat, Bethesda, MD 20892 USA. [Lane, H. Clifford] NIAID, Lab Immunoregulat, Bethesda, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 95.6 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202293 ER PT J AU Jankovic, D Steinfelder, S Andersen, JF Sher, A AF Jankovic, Dragana Steinfelder, Svenja Andersen, John F. Sher, Alan TI Helminth secretory product Ribonuclease T2 is a Th2-inducing agent SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Jankovic, Dragana; Steinfelder, Svenja; Sher, Alan] NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. [Andersen, John F.] NIAID, Lab Malaria & Vector Res, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 43.8 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200201 ER PT J AU Jiang, CC Foley, J Clayton, N Kissling, G Jokinen, M Herbert, R Diaz, M AF Jiang, Chuancang Foley, Julie Clayton, Natasha Kissling, Grace Jokinen, Micheal Herbert, Ronald Diaz, Marilyn TI Abrogation of lupus nephritis in activation-induced cytidine deaminase-deficient MRL/Ipr mice SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Jiang, Chuancang; Diaz, Marilyn] NIEHS, LabMol Genet, NIH, Res Triangle Pk, NC 27709 USA. [Foley, Julie; Clayton, Natasha; Herbert, Ronald] NIEHS, LabExpt Pathol, NIH, Res Triangle Pk, NC 27709 USA. [Kissling, Grace] NIEHS, Biostat Branch, NIH, Res Triangle Pk, NC 27709 USA. [Jokinen, Micheal] Charles River Labs, Pathol Associates, Cary, NC 27513 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 130.3 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200025 ER PT J AU Khaibullina, A Besch, V Middleton, L Quezado, Z AF Khaibullina, Alfia Besch, Virginia Middleton, LaShon Quezado, Zenaide TI Neuronal Nitric Oxide Synthase alters inflammatory response after endotoxin challenge SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Khaibullina, Alfia; Besch, Virginia; Middleton, LaShon; Quezado, Zenaide] NIH, Dept Anesthesia & Surg Serv, Ctr Clin, Bethesda, MD 20892 USA. RI Quezado, Zenaide/O-4860-2016 OI Quezado, Zenaide/0000-0001-9793-4368 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 96.16 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200287 ER PT J AU Kittipatarin, C Khaled, A AF Kittipatarin, Christina Khaled, Annette TI Cytokines Maintain Lymphocyte Homeostasis through the Activity of Cdc25A SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Kittipatarin, Christina; Khaled, Annette] Univ Cent Florida, BioMol Sci Ctr, Orlando, FL 32826 USA. [Khaled, Annette] NCI, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA B56 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200266 ER PT J AU Kole, HK Bolland, S AF Kole, Hemanta K. Bolland, Silvia TI Cloning and characterization of nucleolar-specific autoantibodies from a murine model of lupus/scleroderma SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Kole, Hemanta K.; Bolland, Silvia] NIAID, Autoimmun & Funct Genom Sect, LIG, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 129.6 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201258 ER PT J AU Lavoie, TB Hung, F Crisafulli, S Reynolds, D Scarzello, A Moolchan, K Skawinski, M Koltchev, D Pestka, S Young, HA Clarke, WA AF Lavoie, Thomas B. Hung, Finn Crisafulli, Sara Reynolds, Della Scarzello, Anthony Moolchan, Karlene Skawinski, Michael Koltchev, Doranelly Pestka, Sidney Young, Howard A. Clarke, William A. TI Type I Inteferons Differentially Stimulate Interferon-Gamma Secretion in Human Natural Killer Cells. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Lavoie, Thomas B.; Hung, Finn; Crisafulli, Sara; Moolchan, Karlene; Skawinski, Michael; Koltchev, Doranelly; Pestka, Sidney; Clarke, William A.] PBL Biomed Labs, Piscataway, NJ 08854 USA. [Reynolds, Della; Scarzello, Anthony; Young, Howard A.] NCI, Canc & Inflammat Program, Frederick, MD 21701 USA. [Pestka, Sidney] UMDNJ, Robert Woods Johnson Med Sch, Dept Mol Genet Microbiol & Immunol, Piscataway, NJ 08854 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 95.10 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202292 ER PT J AU Le, Y Zhu, BM Hennighausen, L Silberstein, LE AF Le, Yi Zhu, Bing-Mei Hennighausen, Lothar Silberstein, Leslie E. TI SOCS3 regulates B lymphopoiesis at the immature B cell stage SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Le, Yi; Silberstein, Leslie E.] Childrens Hosp Boston, Boston, MA 02115 USA. [Zhu, Bing-Mei; Hennighausen, Lothar] NIDDKD, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 86.4 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203048 ER PT J AU Li, LQ Lee, J Cohen, T Gorivodsky, M Hayes, SM Zhao, YG El-Khoury, D Grinberg, A Westphal, H Love, PE AF Li, LiQi Lee, Jan Cohen, Tsadok Gorivodsky, Marat Hayes, Sandra M. Zhao, Yangu El-Khoury, Dalal Grinberg, Alexander Westphal, Heiner Love, Paul E. TI LIM Domain Binding Protein 1 (Ldb1) is Required for the Maintenance of Hematopoietic Stem Cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Li, LiQi; Lee, Jan; Cohen, Tsadok; Gorivodsky, Marat; Zhao, Yangu; El-Khoury, Dalal; Grinberg, Alexander; Westphal, Heiner; Love, Paul E.] NICHD, NIH, Bethesda, MD 20892 USA. [Hayes, Sandra M.] SUNY Upstate Med Univ, Dept Microbiol & Immunol, Syracuse, NY 13210 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 81.4 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201100 ER PT J AU Liu, KB Din, NU Browing, DD Abrams, SI Yang, DF AF Liu, Kebin Din, Najam Ud Browing, Darren D. Abrams, Scott I. Yang, Dafeng TI LTbR Mediates Cell Contact-Dependent CTL-Directed Anti-Tumor Cytotoxicity SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Liu, Kebin; Din, Najam Ud; Browing, Darren D.; Yang, Dafeng] Med Coll Georgia, Biochem & Mol Biol, Augusta, GA 30912 USA. [Abrams, Scott I.] NCI, Tumor Immunol & Biol, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 50.11 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202326 ER PT J AU Liu, XB Lee, YS Yu, CR Silver, P Caspi, R Igal, G Charles, EE AF Liu, Xuebin Lee, Yunsang Yu, Cheng-rong Silver, Phyllis Caspi, Rachel Igal, Gery Charles, Egwuagu E. TI Loss of STAT3 in CD4+T cells prevents development of experimental autoimmune uveoretinitis (EAU) and recruitment of pathogenic ThIL17 and Th1 cells into the retina SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Liu, Xuebin; Lee, Yunsang; Yu, Cheng-rong; Silver, Phyllis; Caspi, Rachel; Igal, Gery; Charles, Egwuagu E.] NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 85.29 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202158 ER PT J AU Liu, ZG Pesce, JT Hamed, H Alem, F Lin, HX Chaussabel, D Liu, Q Urban, JF Gause, WC AF Liu, Zhugong Pesce, John T. Hamed, Hossein Alem, Farhang Lin, Hongxia Chaussabel, Damien Liu, Qian Urban, Joseph F., Jr. Gause, William C. TI Neutrophils play critical role for the type 2 protective responses against nematode parasite N. brasiliensis SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Liu, Zhugong; Hamed, Hossein; Alem, Farhang; Lin, Hongxia; Liu, Qian; Gause, William C.] Univ Med & Dent New Jersey, Dept Med, Newark, NJ 07101 USA. [Pesce, John T.; Chaussabel, Damien] NIAID, Immunobiol Sect, NIH, Bethesda, MD 20814 USA. [Urban, Joseph F., Jr.] USDA, Nutrient Requirements & Funct Lab, BARC East, Beltsville, MS 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 51.5 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201122 ER PT J AU Logdberg, LE Akerstrom, B Hair, GA Allhorn, M Vikulina, T Kirshenbaum, AS Sundstrom, JB AF Logdberg, Lennart Erik Akerstrom, Bo Hair, Gregory A. Allhorn, Maria Vikulina, Tatyana Kirshenbaum, Arnold S. Sundstrom, J. Bruce TI On Intrinsic Allergenicity. Using Lipocalins To Probe The Mechanisms Of Allergy SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Logdberg, Lennart Erik] Emory Univ SOM, ECLH, Pathol & Lab Med, Atlanta, GA 30308 USA. [Akerstrom, Bo; Allhorn, Maria] Lund Univ, Clin Sci, Lund, Sweden. [Hair, Gregory A.; Sundstrom, J. Bruce] Emory Univ SOM, Pathol & Lab Med, Atlanta, GA 30322 USA. [Vikulina, Tatyana] Emory Univ SOM, Pathol & Lab Med, WBM, Atlanta, GA 30322 USA. [Kirshenbaum, Arnold S.] NIAID, LAD, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 37.20 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202245 ER PT J AU Longo, NS Satorius, C Durandy, A Lipsky, PE AF Longo, Nancy S. Satorius, Colleen Durandy, Anne Lipsky, Peter E. TI Immunoglobulin mutation traits in humans with AID deficiency SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Longo, Nancy S.; Satorius, Colleen; Lipsky, Peter E.] NIAMS, Autoimmun Branch, NIH, Bethesda, MD 20892 USA. [Durandy, Anne] Hop Necker Enfants Malad, F-75730 Paris 15, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA B220 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201019 ER PT J AU Madala, SK Bundoc, VG Hodges, MG Trivedi, S Urban, JF Keane-Myers, A AF Madala, Satish Kumar Bundoc, Virgilio Garcia Hodges, Marcus Gomez Trivedi, Shweta Urban, Joseph F., Jr. Keane-Myers, Andrea TI Toll-like Receptor 4-Dependent activation of Dendritic cells by heme binding protein from Ascaris suum SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Madala, Satish Kumar; Bundoc, Virgilio Garcia; Hodges, Marcus Gomez; Trivedi, Shweta; Keane-Myers, Andrea] NIAID, Lab Allerg Dis, NIH, Allerg Inflammat Sect, Rockville, MD USA. [Urban, Joseph F., Jr.] USDA, Nutr Requirements Lab, BARC EAST, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 3 U2 3 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 90.9 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203110 ER PT J AU Mans, J Tiemessen, CT Robinson, H Balbo, A Schuck, P Natarajan, K Margulies, DH AF Mans, Janet Tiemessen, C. T. Robinson, H. Balbo, A. Schuck, P. Natarajan, Kannan Margulies, D. H. TI X-ray Crystallographic Structures of Murine Cytomegalovirus MHC-I-like Molecules Reveal Distinct Modes of Exploitation of the MHC-I Fold SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Mans, Janet; Tiemessen, C. T.] Univ Witwatersrand, ZA-2131 Johannesburg, South Africa. [Mans, Janet; Natarajan, Kannan; Margulies, D. H.] NIAID, LI, Bethesda, MD 20892 USA. [Balbo, A.; Schuck, P.] NIH, OD, Bethesda, MD 20892 USA. [Robinson, H.] Brookhaven Natl Lab, Biol, Upton, NY 11973 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 93.18 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202046 ER PT J AU Matthews, AGW Kuo, A Ramon-Maiques, S Han, SM Gallardo, M Yang, W Gozani, O Oettinger, M AF Matthews, Adam Goon Wai Kuo, Alex Ramon-Maiques, Santiago Han, Sunmi Gallardo, Mercedes Yang, Wei Gozani, Or Oettinger, Marjorie TI RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Matthews, Adam Goon Wai; Han, Sunmi; Gallardo, Mercedes; Oettinger, Marjorie] Harvard Univ, MGH Dept Mol Biol, Boston, MA 02114 USA. [Kuo, Alex; Gozani, Or] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA. [Ramon-Maiques, Santiago; Yang, Wei] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 35.8 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201064 ER PT J AU Medvedev, A Piao, WJ Rhee, SH Chen, HY Basu, S Wahl, LM Fenton, MJ Vogel, SN AF Medvedev, Andrei Piao, Wenji Rhee, Sang Hoon Chen, Haiyan Basu, Subhendu Wahl, Larry M. Fenton, Matthew J. Vogel, Stefanie N. TI ROLE OF TLR4 TYROSINE PHOSPHORYLATION IN SIGNAL TRANSDUCTION AND ENDOTOXIN TOLERANCE SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Medvedev, Andrei; Piao, Wenji; Chen, Haiyan; Basu, Subhendu; Fenton, Matthew J.; Vogel, Stefanie N.] Univ Maryland, Baltimore, MD 21201 USA. [Rhee, Sang Hoon] Harvard Med Sch, Boston, MA 02215 USA. [Wahl, Larry M.] NIDCR, NIH, Bethesda, MD 20892 USA. [Fenton, Matthew J.] NIAID, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 89.2 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203026 ER PT J AU Pahar, B Piatak, M Wang, XL Lifson, J Montefiori, DC Ling, BH Lackner, A Veazey, R AF Pahar, Bapi Piatak, Michael Wang, Xiaolei Lifson, Jeffrey Montefiori, David C. Ling, Binhua Lackner, Andrew Veazey, Ronald TI Control of viremia and preservation of intestinal CD4+T cells in SHIVsf162P3 infected macaques intravenously challenged with pathogenic SIVmac251 SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Pahar, Bapi; Wang, Xiaolei; Ling, Binhua; Lackner, Andrew; Veazey, Ronald] Tulane Natl Primate Res Ctr, Covington, LA 70433 USA. [Piatak, Michael; Lifson, Jeffrey] NCI Frederick, SIAC Fredrick Inc, Frederick, MD 21702 USA. [Montefiori, David C.] Duke Univ, Med Ctr, Durham, NC 27710 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA B197 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203311 ER PT J AU Pascal, V Yamada, E Martin, MP Alter, G Alffeld, M Carrington, M Anderson, SK McVicar, DW AF Pascal, Veronique Yamada, Eriko Martin, Maureen P. Alter, Galit Alffeld, Marcus Carrington, Mary Anderson, Stephen K. McVicar, Daniel W. TI Functional evidence supporting the predicted role of KIR3DS1 in the host response to HIV: KIR3DS1 is an activating receptor expressed by human Natural Killer cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Pascal, Veronique; Yamada, Eriko; McVicar, Daniel W.] NCI, Canc & Inflammat Program, Frederick, MD 21702 USA. [Martin, Maureen P.; Carrington, Mary] NCI, Lab Genom Divers, SAIC Frederick Inc, Frederick, MD 21702 USA. [Alter, Galit; Alffeld, Marcus] Harvard Med Sch, Partners AIDS Res Ctr, Infect Dis Unit, Massachusetts Gen Hosp,Div AIDS, Boston, MA 02129 USA. [Anderson, Stephen K.] NCI, Canc & Inflammat Program, SAIC Frederick Inc, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 52.5 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200313 ER PT J AU Peng, Z Hiragun, T Qiao, HH Beaven, MA AF Peng, Ze Hiragun, Takaaki Qiao, Huihong Beaven, Michael A. TI Glucocorticoids Suppress Activation of Mast Cells through induction of Inhibitory Regulators of Signaling Pathways SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Peng, Ze; Hiragun, Takaaki; Qiao, Huihong; Beaven, Michael A.] NHLBI, Lab Mol Immunol, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 94.13 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202220 ER PT J AU Pisitkun, P Deane, JA Bolland, S AF Pisitkun, Prapaporn Deane, Jonathan A. Bolland, Silvia TI Phenotypes of TLR7-overexpressing B cells suggest a TLR7-mediated increase in antigen presentation SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Pisitkun, Prapaporn; Deane, Jonathan A.; Bolland, Silvia] NIAID, AFGS, LIG, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 129.11 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201246 ER PT J AU Ragheb, JA Buggage, R Reed, G Levy-Clarke, G Nussenblatt, R AF Ragheb, Jack A. Buggage, Ronald Reed, George Levy-Clarke, Grace Nussenblatt, Robert TI Abrogation of potent humoral vaccine responses in patients on long-term anti-CD25 therapy SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Ragheb, Jack A.; Buggage, Ronald; Reed, George; Levy-Clarke, Grace; Nussenblatt, Robert] NIH, LI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA B196 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203310 ER PT J AU Ramaswamy, M Dumont, C Muppidi, JR Tybulewicz, VL Siegel, RM AF Ramaswamy, Madhu Dumont, Celine Muppidi, Jagan R. Tybulewicz, Victor L. Siegel, Richard M. TI Rac GTPases are required for restimulation-induced CD4+T Cell Death (RICD) SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Ramaswamy, Madhu; Muppidi, Jagan R.; Siegel, Richard M.] NIAMS, Immunoregulat Unit, Autoimmun Branch, NIH, Bethesda, MD 20892 USA. [Dumont, Celine; Tybulewicz, Victor L.] NIMR, Div Immune Cell Biol, London NW7 1AA, England. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 87.20 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203067 ER PT J AU Ramirez, DC Gomez-Mejiba, SE Corbett, JT Mason, RP AF Ramirez, Dario C. Gomez-Mejiba, Sandra E. Corbett, Jean T. Mason, Ronald P. TI Novel immuno-spin trapping-based assay for the analysis of myeloperoxidase SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Ramirez, Dario C.; Gomez-Mejiba, Sandra E.; Corbett, Jean T.; Mason, Ronald P.] NIEHS, LPC, NIH, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 100.13 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202132 ER PT J AU Revilleza, MJR Levin, D Mage, M Teyton, L Shevach, E Margulies, DH Natarajan, K AF Revilleza, Maria Jamela Roxas Levin, Ditza Mage, Michael Teyton, Luc Shevach, Ethan Margulies, David H. Natarajan, Kannan TI T cell receptors and I-Ad/peptide interactions in autoimmune gastritis (AIG) SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Revilleza, Maria Jamela Roxas; Mage, Michael; Shevach, Ethan; Margulies, David H.; Natarajan, Kannan] NIAID, NIH, Bethesda, MD 20892 USA. [Levin, Ditza] Oil Braude Engn Coll, Biotechnol Engn Dept, Karmiel, Israel. [Teyton, Luc] Scripps Res Inst, Dept Immunol, La Jolla, CA 92037 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 130.40 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200040 ER PT J AU Sakamoto, N Tuji, K Muul, LM Lawler, AM Candotti, F Metcalf, JA Tayel, JA Lane, HC Urba, WJ Fox, BA Varki, AP Lunney, JK Rosenberg, AS AF Sakamoto, Norihisa Tuji, Kuzuhide Muul, Linda M. Lawler, Ann M. Candotti, Fabio Metcalf, Julia A. Tayel, Jorge A. Lane, H. Clifford Urba, Walter J. Fox, Bernard A. Varki, Ajit P. Lunney, Joan K. Rosenberg, Amy S. TI Bovine apolipoprotein B-100 is a dominant immunogen in therapeutic cell populations cultured in FCS in mice and humans SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Sakamoto, Norihisa; Rosenberg, Amy S.] US FDA, CDER, Bethesda, MD 20892 USA. [Tuji, Kuzuhide] Okayama Univ, Okayama 7008558, Japan. [Muul, Linda M.; Candotti, Fabio] NHGRI, NIH, Bethesda, MD 20892 USA. [Lawler, Ann M.] Johns Hopkins Univ, Sch Med, Baltimore, MD 21205 USA. [Metcalf, Julia A.; Tayel, Jorge A.; Lane, H. Clifford] NIAID, NIH, Bethesda, MD 20892 USA. [Urba, Walter J.; Fox, Bernard A.] Providence Portland Med Ctr, Providence Portland Medi, Earle A Chiles Res Inst, Robert W Franz Canc Res Ctr, Portland, OR 97213 USA. [Varki, Ajit P.] Univ Calif San Diego, La Jolla, CA 92093 USA. [Lunney, Joan K.] ARS, USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 88.29 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202113 ER PT J AU Savan, R Owens, G Munroe, DJ Young, HA AF Savan, Ram Owens, Garrison Munroe, David J. Young, Howard A. TI Dissecting the role of miRNAs in Natural Killer (NK) cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Savan, Ram; Young, Howard A.] NCI, Canc & Inflammat Program, NIH, Frederick, MD 21701 USA. [Owens, Garrison; Munroe, David J.] NCI, Lab Mol Technol, NIH, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 94.27 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202224 ER PT J AU Shanker, A Brooks, AD Tristan, CA Wine, J Sayers, TJ AF Shanker, Anil Brooks, Alan D. Tristan, Carlos A. Wine, John Sayers, Thomas J. TI Proteasome inhibition promotes tumor cell apoptosis by exogenous TRAIL without blocking anti-tumor immune effectors in vivo SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Shanker, Anil; Brooks, Alan D.; Tristan, Carlos A.; Wine, John; Sayers, Thomas J.] NCI, Expt Immunol Lab, Canc & Inflammat Program, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 50.6 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202319 ER PT J AU Shi, G Vistica, BP Yu, CR Cox, CA Montalvo, V Wawrousek, EF Egwuagu, CE Gery, I AF Shi, G. Vistica, B. P. Yu, C-R. Cox, C. A. Montalvo, V. Wawrousek, E. F. Egwuagu, C. E. Gery, I. TI Differential involvement of Th1 or Th17 cells in pathogenic autoimmune processes triggered by different TLR ligands SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Shi, G.; Vistica, B. P.; Yu, C-R.; Cox, C. A.; Montalvo, V.; Wawrousek, E. F.; Egwuagu, C. E.; Gery, I.] NEI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 129.22 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201276 ER PT J AU Shin, EC Seifert, U Urban, S Truong, KT Feinstone, SM Rice, CM Kloetzel, PM Rehermann, B AF Shin, Eui-Cheol Seifert, Ulrike Urban, Sabrina Truong, Kim-Thuy Feinstone, Stephen M. Rice, Charles M. Kloetzel, Peter-M Rehermann, Barbara TI Proteasome activator and antigen-processing aminopeptidases are regulated by virus induced type I interferon in the hepatitis C virus-infected liver SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Shin, Eui-Cheol; Truong, Kim-Thuy; Rehermann, Barbara] NIDDK, Immunol Sect, LDB, NIH, Bethesda, MD 20892 USA. [Seifert, Ulrike; Urban, Sabrina; Kloetzel, Peter-M] Humboldt Univ, Charite, Inst Biochem, D-10117 Berlin, Germany. [Feinstone, Stephen M.] US FDA, Lab Hepatitis Viruses, CBER, Bethesda, MD 20892 USA. [Rice, Charles M.] Rockefeller Univ, Ctr Study Hepatitis C, New York, NY 10021 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 44.39 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201348 ER PT J AU Singh, V Ji, QY Hurwitz, AA AF Singh, Vinod Ji, Qingyong Hurwitz, Arthur A. TI TRP-2 TCR Transgenic Mice as a Model to Study CD8+T cell Responses to Melanoma Antigens SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Singh, Vinod; Ji, Qingyong; Hurwitz, Arthur A.] NCI, CIP, CCR, LMI, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 50.15 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202332 ER PT J AU Sredni-Kenigsbuch, D Shohat, M Shohat, B Ben-Amitai, D Chan, CC David, M AF Sredni-Kenigsbuch, Dvora Shohat, Michael Shohat, Batia Ben-Amitai, Dan Chan, Chi-Chao David, Michael TI The Immunomodulating Effect of AS101 on Interleukin-10 Production and the Involvement of MAPK Signaling Pathway in Atopic Dermatitis SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Sredni-Kenigsbuch, Dvora] Bar Ilan Univ, Interdisciplinary, IL-52900 Ramat Gan, Israel. [Shohat, Michael; Shohat, Batia; Ben-Amitai, Dan; David, Michael] Schneider Childrens Hosp, Dermatol, IL-52000 Derech Petach Tikva, Petach Tikva, Israel. [Chan, Chi-Chao] NEI, Lab Immunol, NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 37.3 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202247 ER PT J AU Subleski, JJ Hall, VL Weiss, JM Ortaldo, JR Wiltrout, RH AF Subleski, Jeff John Hall, Veronica L. Weiss, Jonathan M. Ortaldo, John R. Wiltrout, Robert H. TI Differential modulation of NK and NKT cells in the liver and spleen following IL-18+IL-12 treatment of mice SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Subleski, Jeff John; Hall, Veronica L.; Weiss, Jonathan M.; Ortaldo, John R.; Wiltrout, Robert H.] NCI, Canc & Inflamat Program, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 98.9 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202028 ER PT J AU Tang, J Zhu, W Uskokovic, M Silver, P Su, S Chan, CC Adorini, L Caspi, R AF Tang, Jun Zhu, Wei Uskokovic, Milan Silver, Phyllis Su, Shaobo Chan, Chi-Chao Adorini, Luciano Caspi, Rachel TI Beneficial effects of Vitamin D receptor agonists on retinal autoimmunity SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Tang, Jun; Zhu, Wei; Silver, Phyllis; Su, Shaobo; Chan, Chi-Chao; Caspi, Rachel] NEI, LI, NIH, Bethesda, MD 20892 USA. [Uskokovic, Milan] Bioxell Inc, Nutley, NJ 07110 USA. [Adorini, Luciano] Bioxell Inc, SPA, I-20132 Milan, Italy. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 131.30 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201307 ER PT J AU Tang, XB Narayanan, S Coligan, JE Borrego, F AF Tang, Xiaobin Narayanan, Sriram Coligan, John E. Borrego, Francisco TI Characterization of the interaction between human LAIR-1 and collagens. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Tang, Xiaobin; Narayanan, Sriram; Coligan, John E.; Borrego, Francisco] NIAID, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 101.8 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200323 ER PT J AU Terabe, M Ambrosino, E Takaku, S Peng, J Miyake, S Halder, R Yamamura, T Kumar, V Berzofsky, J AF Terabe, Masaki Ambrosino, Elena Takaku, Shun Peng, Judy Miyake, Sachiko Halder, Ramesh Yamamura, Takashi Kumar, Vipin Berzofsky, Jay TI Type II NKT cells suppress tumor immunosurveillance enhanced by type I NKT cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Terabe, Masaki; Ambrosino, Elena; Takaku, Shun; Peng, Judy; Berzofsky, Jay] NCI, Bethesda, MD 20892 USA. [Miyake, Sachiko; Yamamura, Takashi] Natl Inst Neurosci, Kodaira, Tokyo 1878502, Japan. [Halder, Ramesh; Kumar, Vipin] Torrey Pines Inst Mol Studies, San Diego, CA 92121 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 50.16 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202347 ER PT J AU Tzeng, SJ Bolland, S Bonvini, E Pierce, S AF Tzeng, Shiang-Jong Bolland, Silvia Bonvini, Ezio Pierce, Susan TI Fc gamma RIIB1 regulates plasma cell apoptosis through a c-Abl-dependent pathway SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Tzeng, Shiang-Jong; Bolland, Silvia; Pierce, Susan] NIAID, Immunogenet, NIH, Rockville, MD 20852 USA. [Bonvini, Ezio] Macrogenics, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 86.8 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203052 ER PT J AU Vandanmagsar, B Yang, H Carter, A Collins, G Pyle, R Bertak, D Taub, D AF Vandanmagsar, Bolormaa Yang, Hyunwon Carter, Arne Collins, Gary Pyle, Robert Bertak, Dorothy Taub, Dennis TI Effects of T-cell derived leptin on thymic function and immune regulation SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Vandanmagsar, Bolormaa; Yang, Hyunwon; Carter, Arne; Collins, Gary; Pyle, Robert; Bertak, Dorothy; Taub, Dennis] NIA, LI, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 85.8 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202139 ER PT J AU Viekind, S Long, E AF Viekind, Susina Long, Eric TI Regulation of IL-15 trans-presentation to NK cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Viekind, Susina; Long, Eric] NIAID, LIG, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 94.18 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202227 ER PT J AU Wan, FY Zheng, LX Anderson, DE Lam, LT Hegde, V Deutsch, WA Staudt, LM Lenardo, MJ AF Wan, Fengyi Zheng, Lixin Anderson, D. Eric Lam, Lloyd T. Hegde, Vijay Deutsch, Walter A. Staudt, Louis M. Lenardo, Michael J. TI Identification a novel NF-kB regulator by tandem affinity purification combined with mass spectrometry SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Wan, Fengyi; Zheng, Lixin; Lenardo, Michael J.] NIAID, Lab Immunol, NIH, Bethesda, MD 20892 USA. [Anderson, D. Eric] NIDDK, Prote & Mass Spectrometry Facil, NIH, Bethesda, MD 20892 USA. [Lam, Lloyd T.; Staudt, Louis M.] NCI, Metab Branch, NIH, Bethesda, MD 20892 USA. [Hegde, Vijay; Deutsch, Walter A.] Louisiana State Univ Syst, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 35.44 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201068 ER PT J AU Wang, HS Feng, JX Morse, HC AF Wang, Hongsheng Feng, Jianxun Morse, Herbert C., III TI Functional deficiency in IL-7 caused by an ENU-induced point mutation SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Wang, Hongsheng; Feng, Jianxun; Morse, Herbert C., III] NIAID, Immunopathol Lab, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 82.11 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200335 ER PT J AU Wang, JM Li, YY Hu, JY Huang, J Gong, WH Chen, KQ AF Wang, Ji Ming Li, Yingying Hu, Jinyue Huang, Jian Gong, Wanghua Chen, Keqiang TI The key role of a G protein coupled receptor mFPR2 in the development of inflammatory airway disease SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Wang, Ji Ming; Huang, Jian; Chen, Keqiang] NCI, Mol Immunoregulat Lab, Frederick, MD 21702 USA. [Li, Yingying] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China. [Hu, Jinyue] Cent S Univ, Xiang Ya Sch Med, Changsha, Hunan, Peoples R China. [Gong, Wanghua] NCI, SAIC Frederick, Basic Res Program, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 98.4 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202031 ER PT J AU Wang, Q Hanada, K Yang, JC AF Wang, Qiong Hanada, Ken-ichi Yang, James C. TI TNF-related apoptosis inducing ligand (TRAIL) enhances TCR-mediated renal cancer recognition by a novel CD4+T cell clone SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Wang, Qiong; Hanada, Ken-ichi; Yang, James C.] NIH, Surg Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 50.29 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202328 ER PT J AU Whittaker, GC Quigley, L Zhang, WG McVicar, D AF Whittaker, Gillian Claire Quigley, Laura Zhang, Weigou McVicar, Daniel TI LAB/NTAL Regulates DAP12 Signaling and Macrophage Differentiation SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Whittaker, Gillian Claire; Quigley, Laura; McVicar, Daniel] NCI, Canc Inflammat Program, Frederick, MD 21702 USA. [Zhang, Weigou] Duke Univ, Med Ctr, Immunol, Durham, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA B163 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201137 ER PT J AU Williams, E Stacy, S Carlisle, P Infante, A Lenardo, M Kraig, E AF Williams, Earlanda Stacy, Sue Carlisle, Patricia Infante, Anthony Lenardo, Michael Kraig, Ellen TI T cell tolerance to AChR, the autoantigen in myasthenia gravis, is mediated primarily by peripheral mechanisms SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Williams, Earlanda; Stacy, Sue; Carlisle, Patricia; Infante, Anthony; Kraig, Ellen] Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA. [Lenardo, Michael] NIAID, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 128.25 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203176 ER PT J AU Wuest, T Luster, A Campbell, I Farber, J Carr, D AF Wuest, Todd Luster, Andrew Campbell, Iain Farber, Joshua Carr, Daniel TI CXCL10 is required for the recruitment of leukocytes responsible for controlling herpes simplex virus-1 infection SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Wuest, Todd] OUHSC, Dept Ophthalmol, Microbiol & Immunol, Oklahoma City, OK 73104 USA. [Luster, Andrew] Harvard Med Sch, Massachusettts Gen Hosp, Rheum Allergy & Immunol, Boston, MA USA. [Campbell, Iain] Univ Sydney, Mol Biol, Sydney, NSW 2006, Australia. [Farber, Joshua] NIAID, NIH, Bethesda, MD 20892 USA. [Carr, Daniel] OUSHC, Ophthalmol, Oklahoma City, OK 73104 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 43.19 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200217 ER PT J AU Xie, ZH Johnson, EN Druey, KM AF Xie, Zhihui Johnson, Eric N. Druey, Kirk M. TI Rgs13 acts as a nuclear repressor of CREB in B lymphocytes SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Xie, Zhihui; Druey, Kirk M.] NIH, Lab Allerg Dis, Bethesda, MD 20892 USA. [Johnson, Eric N.] Merck & Co Inc, N Wales, PA 19454 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA B10 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201201 ER PT J AU Yang, DF Smith, KK Georgi, D Abrams, SI Liu, KB AF Yang, Dafeng Smith, Kimberly K. Georgi, David Abrams, Scott I. Liu, Kebin TI Cooperative downregulation of IFN[gamma]R and Fas is functionally linked to CTL-mediated immunoselection and tumor promotion SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Yang, Dafeng; Liu, Kebin] Med Coll Georgia, Dept Biochem & Mol Biol, Augusta, GA 30912 USA. [Smith, Kimberly K.; Georgi, David] Med Coll Georgia, Dept Pathol, Augusta, GA 30912 USA. [Abrams, Scott I.] NCI, Lab Tumor Immunol & Biol, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 50.3 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202341 ER PT J AU Yu, CR Amadi-Obi, A Mahdi, RM Liu, XB Lee, Y Gery, I Egwuagu, C AF Yu, Cheng-Rong Amadi-Obi, Ahjoko Mahdi, Rashid M. Liu, Xuebin Lee, Yunsang Gery, Igal Egwuagu, Charles TI Constitutive expression of IL-27 in neuro-retina negatively regulates THIL-17 cell development SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Yu, Cheng-Rong; Amadi-Obi, Ahjoko; Mahdi, Rashid M.; Liu, Xuebin; Lee, Yunsang; Gery, Igal; Egwuagu, Charles] NEI, Immunol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 95.25 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202315 ER PT J AU Yucesoy, B Johnson, VJ Fluharty, K Kashon, ML Slaven, J Kissling, G Germolec, D Vallyathan, V Luster, MI AF Yucesoy, Berran Johnson, Victor J. Fluharty, Kara Kashon, Michael L. Slaven, James Kissling, Grace Germolec, Dori Vallyathan, Val Luster, Michael I. TI Association of genetic variations in inflammatory and fibrogenic genes with progressive massive fibrosis in coal miners SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Yucesoy, Berran; Johnson, Victor J.; Fluharty, Kara; Luster, Michael I.] CDC, Toxicol & Mol Biol Branch, NIOSH, Morgantown, WV 26505 USA. [Kashon, Michael L.; Slaven, James] CDC, Biostat & Epidemiol Branch, NIOSH, Morgantown, WV 26505 USA. [Vallyathan, Val] CDC, Pathol & Physiol Res Branch, NIOSH, Morgantown, WV 26505 USA. [Germolec, Dori] NIEHS, Toxicol Operat Branch, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 35.51 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758201035 ER PT J AU Zhang, H Snyder, K Suhoski, M Maus, M Kapoor, V June, CH Mackall, CL AF Zhang, Hua Snyder, Kristen Suhoski, Megan Maus, Marcela Kapoor, Veena June, Carl H. Mackall, Crystal L. TI 4-1BB is Superior to CD28 Costimulation for Generating Effector CD8+T Cells for Adoptive Immunotherapy SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Zhang, Hua; Snyder, Kristen; Mackall, Crystal L.] NCI, Pediat Oncol Branch, Bethesda, MD 20892 USA. [Suhoski, Megan; Maus, Marcela; June, Carl H.] Univ Penn, Abramson Family Canc Res Inst, Philadelphia, PA 19104 USA. [Kapoor, Veena] NCI, Expt Transplantat & Immunol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 48.12 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758203302 ER PT J AU Zhou, JH Nagarkatti, P Robbins, PF Rosenberg, SA Nagarkatti, M AF Zhou, Juhua Nagarkatti, Prakash Robbins, Paul F. Rosenberg, Steven A. Nagarkatti, Mitzi TI Memory T cells in the tumor-infiltrating lymphocytes for adoptive cell transfer therapy SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Zhou, Juhua; Nagarkatti, Prakash; Nagarkatti, Mitzi] Univ South Carolina, Pathol Microbiol & Immunol, Columbia, SC 29209 USA. [Robbins, Paul F.; Rosenberg, Steven A.] NCI, Surgery Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA B145 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200135 ER PT J AU Zhu, Q Belyakov, IM Klinman, DM Berzofsky, JA AF Zhu, Qing Belyakov, Igor M. Klinman, Dennis M. Berzofsky, Jay A. TI In vivo synergy of TLR ligands through activation of distinct signaling pathways in dendritic cells SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Zhu, Qing; Belyakov, Igor M.; Berzofsky, Jay A.] NCI, Vaccine Branch, NIH, Bethesda, MD 20892 USA. [Klinman, Dennis M.] US FDA, Sect Retroviral Res, CBER, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 52.1 PG 2 WC Immunology SC Immunology GA V44OL UT WOS:000209758200300 ER PT J AU Zhu, XY Liu, B Han, KP Lee, HI You, LJ Rhode, PR Morgan, RA Wong, HC AF Zhu, Xiaoyun Liu, Bai Han, Kaiping Lee, Hyung-il You, Lijing Rhode, Peter R. Morgan, Richard A. Wong, Hing C. TI Recognition of Melanoma-Associated Antigens gp100 or Mart1/Melan-A in the Context of HLA-A2 by Soluble Single Chain T-Cell Receptor Mu!timers SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Zhu, Xiaoyun; Liu, Bai; Han, Kaiping; Lee, Hyung-il; You, Lijing; Rhode, Peter R.; Wong, Hing C.] Altor BioSci Corp, Miramar, FL 33025 USA. [Morgan, Richard A.] NCI, Surg Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 2007 VL 178 SU 1 MA 93.15 PG 1 WC Immunology SC Immunology GA V44OL UT WOS:000209758202049 ER PT J AU Ahmadzadeh, M Antony, PA Rosenberg, SA AF Ahmadzadeh, Mojgan Antony, Paul A. Rosenberg, Steven A. TI IL-2 and IL-15 each mediate de novo induction of FOXP3 expression in human tumor antigen-specific CD8 T cells SO JOURNAL OF IMMUNOTHERAPY LA English DT Article DE FOXP3; memory T cells; regulatory T cells; IL-2; IL-15; human; tumor-Ag ID TRANSCRIPTION FACTOR FOXP3; REGULATORY CELLS; HELPER-CELLS; IN-VIVO; TOLERANCE; MELANOMA; INTERLEUKIN-2; IMMUNOTHERAPY; ACQUISITION; ACTIVATION AB Although FOXP3 is primarily expressed by regulatory CD4 T cells (T-reg) in vivo, polyclonal activation of human CD8 T cells can result in the expression of FOXP3 in a fraction of CD8 T cells. However, the cellular lineage and mechanism of FOXP3 induction in CD8 T cells remain unclear. Here, we demonstrate that interleukin-2 (IL-2) induces FOXP3 expression in OKT3-stimulated or antigen-stimulated CD8 T cells, indicating that FOXP3 expression is neither limited to a unique subset of CD8 T cells nor dependent on the mode of T-cell receptor stimulation. In the absence of IL-2, antigen stimulation resulted in T-cell activation and acquisition of effector function without induction of FOXP3, indicating that acquisition of effector function is independent of induction of FOXP3 expression in CD8 T cells. Interestingly, IL-15, but not IL-7 or IL-21, also led to de novo induction of FOXP3 in antigen-specific CD8 T cells, suggesting that signaling by IL-2/IL-15R beta chain is pivotal for induction of FOXP3 in human CD8 T cells. These findings indicate that induction of FOXP3 is intrinsic to CD8 T cells that are activated in the presence of IL-2 or IL-15, and in vitro-induced expression of FOXP3 cannot be simply interpreted as an indicator of T-reg activity or activation marker. C1 NCI, Surg Branch, NIH, Bethesda, MD 20892 USA. RP Rosenberg, SA (reprint author), NCI, Surg Branch, NIH, CRC Bldg,Room 3W-2940,10 Ctr Dr, Bethesda, MD 20892 USA. EM sar@nih.gov FU Intramural NIH HHS [Z01 SC003811-32] NR 34 TC 36 Z9 37 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1524-9557 J9 J IMMUNOTHER JI J. Immunother. PD APR PY 2007 VL 30 IS 3 BP 294 EP 302 DI 10.1097/CJI.0b013e3180336787 PG 9 WC Oncology; Immunology; Medicine, Research & Experimental SC Oncology; Immunology; Research & Experimental Medicine GA 151KO UT WOS:000245289100005 PM 17414320 ER PT J AU Morens, DM Fauci, AS AF Morens, David M. Fauci, Anthony S. TI The 1918 influenza pandemic: Insights for the 21st century SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID HANTAVIRUS PULMONARY SYNDROME; RESPIRATORY-DISTRESS-SYNDROME; HONG-KONG INFLUENZA; HOST IMMUNE; VIRUS; HEMAGGLUTININ; NEURAMINIDASE; PATHOGENESIS; PNEUMONIA; MORTALITY AB The 1918-1919 H1N1 influenza pandemic was among the most deadly events in recorded human history, killing an estimated 50-100 million persons. Because recent H5N1 avian epizootics have been associated with sporadic human fatalities, concern has been raised that a new pandemic, as fatal as the pandemic of 1918, or more so, could be developing. Understanding the events and experiences of 1918 is thus of great importance. However, despite the genetic sequencing of the entire genome of the 1918 virus, many questions about the 1918 pandemic remain. In this review we address several of these questions, concerning pandemic-virus origin, unusual epidemiologic features, and the causes and demographic patterns of fatality. That none of these questions can yet be fully answered points to the need for continued pandemic vigilance, basic and applied research, and pandemic preparedness planning that emphasizes prevention, containment, and treatment with antiviral medications and hospital-based intensive care. C1 NIAID, NIH, Bethesda, MD 20892 USA. RP Morens, DM (reprint author), NIAID, NIH, 31 Ctr Dr,Bldg 31,Rm 7A-02 MSC 2520, Bethesda, MD 20892 USA. EM dm270q@nih.gov NR 100 TC 181 Z9 189 U1 3 U2 17 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD APR 1 PY 2007 VL 195 IS 7 BP 1018 EP 1028 DI 10.1086/511989 PG 11 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 143KZ UT WOS:000244721700016 PM 17330793 ER PT J AU Ando, H Kondoh, H Ichihashi, M Hearing, VJ AF Ando, Hideya Kondoh, Hirofumi Ichihashi, Masamitsu Hearing, Vincent J. TI Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Review ID ER-ASSOCIATED DEGRADATION; NECROSIS-FACTOR-ALPHA; OCULOCUTANEOUS ALBINISM TYPE-1; UBIQUITIN-PROTEASOME PATHWAY; MELANOCYTE CELL-CULTURES; EARLY SECRETORY PATHWAY; MESSENGER-RNA LEVELS; ENDOPLASMIC-RETICULUM; MISFOLDED GLYCOPROTEINS; DOWN-REGULATION AB Tyrosinase, a copper-containing glycoprotein, is the rate-limiting enzyme critical for melanin biosynthesis in specialized organelles termed melanosomes that are produced only by melanocytic cells. Inhibitors of tyrosinase activity have long been sought as therapeutic means to treat cutaneous hyperpigmentary disorders. Multiple potential approaches exist that could control pigmentation via the regulation of tyrosinase activity, for example: the transcription of its messenger RNA, its maturation via glycosylation, its trafficking to melanosomes, as well as modulation of its catalytic activity and/or stability. However, relatively little attention has been paid to regulating pigmentation via the stability of tyrosinase, which depends on its processing and maturation in the endoplasmic reticulum and Golgi, its delivery to melanosomes and its degradation via the ubiquitin-proteasome pathway and/or the endosomal/lysosomal system. Recently, it has been shown that carbohydrate modification, molecular chaperone engagement, and ubiquitylation all play pivotal roles in regulating the degradation/stability of tyrosinase. While such processes affect virtually all proteins, such effects on tyrosinase have immediate and dramatic consequences on pigmentation. In this review, we classify melanogenic inhibitory factors in terms of their modulation of tyrosinase function and we summarize current understanding of how the quality control of tyrosinase processing impacts its stability and melanogenic activity. C1 NCI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. Kobe Skin Res Inst, Kobe, Hyogo, Japan. BioRes Inc, Kobe, Hyogo, Japan. Sun Care Res Inst, Osaka, Japan. RP Hearing, VJ (reprint author), NCI, Cell Biol Lab, NIH, Bldg 37,Room 2132,MSC 4256, Bethesda, MD 20892 USA. EM hearingv@nih.gov NR 145 TC 127 Z9 136 U1 2 U2 29 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 IS 4 BP 751 EP 761 DI 10.1038/sj.jid.5700683 PG 11 WC Dermatology SC Dermatology GA 151TV UT WOS:000245314600006 PM 17218941 ER PT J AU Aszterbaum, M Bickers, D Cappola, C Estevez, N Epstein, E Hawk, E Hwang, J Kopelovich, L Lu, Y AF Aszterbaum, M. Bickers, D. Cappola, C. Estevez, N. Epstein, E. Hawk, E. Hwang, J. Kopelovich, L. Lu, Y. TI Basal cell nevus syndrome: resistance to chemopreventive effects of oral celecoxib SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Univ Calif San Francisco, San Francisco, CA 94143 USA. Univ Calif San Francisco, Ctr Comprehens Canc, San Francisco, CA 94143 USA. Columbia Univ, Coll Phys & Surg, New York, NY USA. NCI, Div Canc Prevent & Control, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 245 BP S41 EP S41 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800242 ER PT J AU Athar, M Tang, X Kim, KH Kim, AL Kopelovich, L Bickers, DR AF Athar, M. Tang, X. Kim, K. H. Kim, A. L. Kopelovich, L. Bickers, D. R. TI Interaction of p53 and sonic hedgehog signaling pathways in the development of ultraviolet-B (UVB)-induced basal cell carcinomas (BCCs) in murine skin SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Columbia Univ, New York, NY USA. NCI, Div Canc Prevent, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 150 BP S25 EP S25 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800150 ER PT J AU Bickers, DR Tang, X Kim, AL Zhu, Y Kopelovich, L Athar, M AF Bickers, D. R. Tang, X. Kim, A. L. Zhu, Y. Kopelovich, L. Athar, M. TI Nuclear factor Kappa B (NF kappa B) blockade inhibits its activation by ultraviolet B (UVB) and UVB-induced photocarcinogenesis in murine skin SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Columbia Univ, New York, NY USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 153 BP S26 EP S26 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800152 ER PT J AU Bonnart, C Deraison, C Besson, C Briot, A Robin, A Gonthier, M Monsarrat, B Segre, J Hovnanian, A AF Bonnart, C. Deraison, C. Besson, C. Briot, A. Robin, A. Gonthier, M. Monsarrat, B. Segre, J. Hovnanian, A. TI Elastase 2 is a new epidermal serine protease that contributes to skin barrier function in vivo SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 INSERM, U563, Toulouse, France. CNRS IPBS, Toulouse, France. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 435 BP S73 EP S73 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800431 ER PT J AU Cataisson, C Ohman, R Pearson, A Tsien, M Hennings, H Yuspa, SH AF Cataisson, C. Ohman, R. Pearson, A. Tsien, M. Hennings, H. Yuspa, S. H. TI Inflammation and skin cancer are linked by protein kinase C alpha in a mouse model for inducible cutaneous inflammation SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, Lab Canc Biol & Genet, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 144 BP S24 EP S24 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800144 ER PT J AU Cho, Y Miyagawa, F Katz, SI AF Cho, Y. Miyagawa, F. Katz, S. I. TI CD3(-)CD11c(+)CD4(+) splenic DCs can cross present keratinocyte cell membrane-associated antigen to CD8(+) T cells in K14-mOVA Tg mice in vitro SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 713 BP S119 EP S119 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800706 ER PT J AU Clark, J Turner, ML Kopp, JB AF Clark, J. Turner, M. L. Kopp, J. B. TI Inheritance pattern in familial keloids SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, NIDDK, Bethesda, MD 20892 USA. Duke Univ, Sch Med, Durham, NC USA. NIH, Natl Canc Inst, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 565 BP S95 EP S95 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800561 ER PT J AU Cowen, EW Liu, C Steinberg, SM Kang, S Vonderheid, EC Kwak, H Booher, S Petricoin, EF Liotta, LA Hwang, ST AF Cowen, E. W. Liu, C. Steinberg, S. M. Kang, S. Vonderheid, E. C. Kwak, H. Booher, S. Petricoin, E. F. Liotta, L. A. Hwang, S. T. TI Differentiation of tumor-phase mycosis fungoides, psoriasis vulgaris, and normal controls in a pilot study using serum proteomic analysis SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Natl Canc Inst, NIH, Bethesda, MD USA. SAIC Frederick Inc, Clin Proteom Reference Lab, Gaithersburg, MD USA. Natl Canc Inst, NIH, Biostat & Data Management Sect, Rockville, MD USA. Univ Michigan, Sch Med, Dept Dermatol, Ann Arbor, MI USA. Johns Hopkins Med Inst, Dept Dermatol, Baltimore, MD 21205 USA. Johns Hopkins Med Inst, Dept Oncol, Baltimore, MD 21205 USA. George Mason Univ, Ctr Appl Proteom & Mol Med, Dept Mol Microbiol, Manassas, VA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 227 BP S38 EP S38 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800224 ER PT J AU De Guzman Strong, C Patel, S Wertz, PW Wang, C Yang, F Meltzer, PS Andl, T Millar, SE Ho, I Pai, S Segre, JA AF De Guzman Strong, C. Patel, S. Wertz, P. W. Wang, C. Yang, F. Meltzer, P. S. Andl, T. Millar, S. E. Ho, I. Pai, S. Segre, J. A. TI Epidermal-specific deletion of GATA-3 results in selective barrier deficiency SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NHGRI, Bethesda, MD 20892 USA. Natl Canc Inst, Bethesda, MD USA. Univ Iowa, Iowa City, IA USA. Univ Penn, Philadelphia, PA 19104 USA. Brigham & Womens Hosp, Boston, MA 02115 USA. Childrens Hosp, Dana Farber Canc Inst, Boston, MA 02115 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 573 BP S96 EP S96 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800568 ER PT J AU Deraison, C Bonnart, C Robin, A Briot, A Besson, C Segre, J Hovnanian, A AF Deraison, C. Bonnart, C. Robin, A. Briot, A. Besson, C. Segre, J. Hovnanian, A. TI Transgenic mice provide in vivo evidence that KLK5 initiates the proteolytic cascade of desquamation SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 INSERM, U563, Toulouse, France. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 436 BP S73 EP S73 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800434 ER PT J AU Duverger, O Lee, D Morasso, M AF Duverger, O. Lee, D. Morasso, M. TI Study of the functional alterations in the mutated DLX3 isoform responsible for Tricho-Dento-Osseous syndrome SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH NIAMS, Dev Skin Biol Unit, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 551 BP S92 EP S92 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800545 ER PT J AU Faghri, S DiGiovanna, JJ Tamura, D Kraemer, KH AF Faghri, S. DiGiovanna, J. J. Tamura, D. Kraemer, K. H. TI Trichothiodystrophy includes a broad spectrum of multisystem abnormalities and may have a high mortality at a young age SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, Basic Res Labs, Bethesda, MD 20892 USA. Brown Med Sch, Providence, RI USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 631 BP S106 EP S106 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800626 ER PT J AU Falanga, V Iwamoto, S Chartier, M Yufit, T Butmarc, J Kouttab, N Colvin, G Carson, P AF Falanga, V. Iwamoto, S. Chartier, M. Yufit, T. Butmarc, J. Kouttab, N. Colvin, G. Carson, P. TI Autologous bone marrow-derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Roger Williams Med Ctr, Providence, RI USA. Roger Williams Med Ctr, Ctr Biomed Res Excellence, Providence, RI USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 293 BP S49 EP S49 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800288 ER PT J AU Fang, L Hwang, ST AF Fang, L. Hwang, S. T. TI CCR2 and CCR7 regulate B16 murine melanoma cell tumorigenesis in skin SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, Dermatol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 752 BP S126 EP S126 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800749 ER PT J AU Gaffal, E Landsberg, J Kohlmeyer, J Tormo, D Wenzel, J Merlino, G Tuting, T AF Gaffal, E. Landsberg, J. Kohlmeyer, J. Tormo, D. Wenzel, J. Merlino, G. Tueting, T. TI Mutant CDK4 increases the penetrance and decreases the latency of UV-induced, metastasizing melanoma in the skin of C57BL/6 HGF mice SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Univ Bonn, D-5300 Bonn, Germany. NCI, Lab Cell Regulat & Carcinogenesis, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 111 BP S19 EP S19 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800111 ER PT J AU Griffiths, PE Shi, B Sivamani, RK Holmes, C Goldstein, DS Isseroff, RR AF Griffiths, P. E. Shi, B. Sivamani, R. K. Holmes, C. Goldstein, D. S. Isseroff, R. R. TI Wounding alters the beta2-adrenergic signaling pathway in cultured keratinocytes SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Univ Calif Davis, Dept Dermatol, Davis, CA 95616 USA. NIH, NINDS, Clin Neurocardiol Sect, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 613 BP S103 EP S103 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800609 ER PT J AU Hastings, K Irvine, K Antony, P Restifo, N Cresswell, P AF Hastings, K. Irvine, K. Antony, P. Restifo, N. Cresswell, P. TI Lysosomal thiol reductase GILT is essential for MHC class II-restricted processing of melanoma antigen TRP-1 SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Univ Arizona, Coll Med, Dept Basic Med Sci, Phoenix, AZ USA. NIH, NCI, Surg Branch, Bethesda, MD 20892 USA. Johns Hopkins Univ, Sch Med, Baltimore, MD USA. Yale Univ, Sch Med, Dept Immunobiol, New Haven, CT USA. RI Restifo, Nicholas/A-5713-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 767 BP S128 EP S128 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800760 ER PT J AU He, Y Pi, J Huang, J Diwan, BA Waalkes, MP Chignell, CF AF He, Y. Pi, J. Huang, J. Diwan, B. A. Waalkes, M. P. Chignell, C. F. TI Chronic UVA irradiation of human HaCaT keratinocytes induces malignant transformation: role of PI3K/PTEN/AKT signaling SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, NIEHS, LPC, Res Triangle Pk, NC USA. NIH, NIEHS, NCI NIH, LCC, Res Triangle Pk, NC USA. NIH, NCI, Sci Applicat Int Corp, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 112 BP S19 EP S19 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800114 ER PT J AU Hong, C Takeuchi, F Aldrich, S Hathaway, L Moss, J Lee, C Darling, TN AF Hong, C. Takeuchi, F. Aldrich, S. Hathaway, L. Moss, J. Lee, C. Darling, T. N. TI Correlations among different types of skin lesions and internal organ involvement in tuberous sclerosis complex SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Tainan Hosp, Dept Hlth, Tainan, Taiwan. Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA. NIH, NHLBI, Pulm Crit Care Med Branch, Bethesda, MD 20892 USA. Kaohsiung Med Univ, Kaohsiung, Taiwan. NIH, NCI, Dermatol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 279 BP S47 EP S47 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800275 ER PT J AU Huang, V Kakinuma, T Murakami, T Huang, S AF Huang, V. Kakinuma, T. Murakami, T. Huang, S. TI Accumulation of CCL27 protein in skin-draining lymph node (LN) in the absence of CCL27 transcription may contribute to CCR10-mediated nodal metastasis SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, Dermatol Branch, Bethesda, MD 20892 USA. NCI, CCR, Dermatol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 149 BP S25 EP S25 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800147 ER PT J AU Hwang, J Morasso, M AF Hwang, J. Morasso, M. TI Dlx3 gene expression in lacZ knock-in mice and its function in hair development SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, NIAMS, Dev Skin Biol Unit, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 636 BP S106 EP S106 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800631 ER PT J AU Imoto, K Boyle, J Oh, K Khan, S Ueda, T Nadem, C Slor, H Orgal, S Gadoth, N Busch, D Jaspers, NG Tamura, D DiGiovanna, JJ Kraemer, KH AF Imoto, K. Boyle, J. Oh, K. Khan, S. Ueda, T. Nadem, C. Slor, H. Orgal, S. Gadoth, N. Busch, D. Jaspers, N. G. Tamura, D. DiGiovanna, J. J. Kraemer, K. H. TI Patients with defects in the interacting nucleotide excision repair proteins ERCC1 or XPF show xeroderma pigmentosum with late onset severe neurological degeneration SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, Basic Res Labs, Bethesda, MD 20892 USA. Nara Med Univ, Nara, Japan. Tel Aviv Univ, IL-69978 Tel Aviv, Israel. AFIP, Washington, DC USA. Erasmus Univ, Rotterdam, Netherlands. Brown Univ, Sch Med, Providence, RI 02912 USA. NR 0 TC 5 Z9 5 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 547 BP S92 EP S92 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800543 ER PT J AU Kalinin, O Hwang, J Hwang, M Morasso, MI AF Kalinin, O. Hwang, J. Hwang, M. Morasso, M. I. TI Ca++-binding protein Scarf function during epidermal development SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, NIAMS, Dev Skin Biol Unit, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 448 BP S75 EP S75 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800446 ER PT J AU Khan, SG Yamanegi, K Zheng, Z Boyle, J Imoto, K Oh, KS Armstrong, N Baker, CC Kraemer, KH AF Khan, S. G. Yamanegi, K. Zheng, Z. Boyle, J. Imoto, K. Oh, K. S. Armstrong, N. Baker, C. C. Kraemer, K. H. TI Mutations within two branch point sequence (BPS) in an intron of the XPC gene disrupt U2 snRNP - BPS interaction resulting in mild versus severe XP phenotypes SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Natl Canc Inst, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 138 BP S23 EP S23 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800139 ER PT J AU Kim, KH Zhu, Y Back, JH Tang, X Kopelovich, L Athar, M Kim, AL Bickers, DR AF Kim, K. H. Zhu, Y. Back, J. H. Tang, X. Kopelovich, L. Athar, M. Kim, A. L. Bickers, D. R. TI Resveratrol suppresses ultraviolet B (UVB)-induced SCC formation in p53+/-/SKH-1 hairless mice through modulation of mediators of epithelial to mesenchymal transition (EMT) SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Columbia Univ, Dept Dermatol, New York, NY 10027 USA. Hallym Univ, Coll Med, Anyang, South Korea. NCI, Div Canc Prevent, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 169 BP S29 EP S29 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800169 ER PT J AU Lee, C Fang, L Palmer, D Restifo, N Hwang, S AF Lee, C. Fang, L. Palmer, D. Restifo, N. Hwang, S. TI A CXCR4 chemokine receptor antagonist enhances antigen-specific, adoptive immunotherapy for established B16 melanoma tumors SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, Dermatol Branch, CCR, NIH, Bethesda, MD 20892 USA. Kaohsiung Med Univ, Kaohsiung, Taiwan. NCI, Surg Branch, CCR, NIH, Bethesda, MD 20892 USA. RI Restifo, Nicholas/A-5713-2008; Lee, Chih-Hung /B-4081-2010 NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 691 BP S116 EP S116 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800686 ER PT J AU Lee, D Duverger, O Jaisser, F Morasso, M AF Lee, D. Duverger, O. Jaisser, F. Morasso, M. TI Developing a tetracycline-inducible system for analyzing Dlx3 function in mouse keratinocytes SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, NIAMS, Dev Skin Biol Unit, Bethesda, MD 20892 USA. Coll France, INSERM, U772, F-75231 Paris, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 444 BP S74 EP S74 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800441 ER PT J AU Leitner, WW Baker, MC Yannie, JP Udey, MC AF Leitner, W. W. Baker, M. C. Yannie, J. P. Udey, M. C. TI Enhancement of DNA vaccine efficacy by simple gene gun mediated co-delivery of minute amounts of a plasmid-encoded helper antigen SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, Udey Dermatol Branch, Bethesda, MD 20892 USA. RI Leitner, Wolfgang/F-5741-2011 OI Leitner, Wolfgang/0000-0003-3125-5922 NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 720 BP S120 EP S120 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800715 ER PT J AU Mascia, F Mariani, V Cataisson, C Yuspa, S Pastore, S AF Mascia, F. Mariani, V. Cataisson, C. Yuspa, S. Pastore, S. TI Granulocyte-macrophage colony-stimulating factor expression is regulated by epidermal growth factor receptor pathway SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, NCI, LCGB Lab, Bethesda, MD 20892 USA. IDI, Lab Cutaneous Physiopathol, Rome, Italy. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 581 BP S97 EP S97 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800575 ER PT J AU Miyagawa, F Tagaya, Y Waldmann, TA Katz, SI AF Miyagawa, F. Tagaya, Y. Waldmann, T. A. Katz, S. I. TI IL-15 serves as a co-stimulator in determining the activity of autoreactive CD8 T cells in an experimental mouse model of graft vs. host disease (GvHD) SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, Dermatol Branch, Bethesda, MD 20892 USA. NIH, Metab Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 697 BP S117 EP S117 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800692 ER PT J AU Moore, EC Lonsdorf, AS Hwang, ST Moon, RT Chien, AJ AF Moore, E. C. Lonsdorf, A. S. Hwang, S. T. Moon, R. T. Chien, A. J. TI The effects of Wnt/beta-catenin signaling in a murine melanoma model SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Univ Washington, Sch Med, Seattle, WA USA. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 611 BP S102 EP S102 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800605 ER PT J AU Nagao, K Neutzner, M Leitner, WW Sardy, M Lu, M Clausen, BE Udey, MC AF Nagao, K. Neutzner, M. Leitner, W. W. Sardy, M. Lu, M. Clausen, B. E. Udey, M. C. TI Langerhans cells represent the predominant source of MFG-E8 in skin SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, NIH, Dermatol Branch, Bethesda, MD 20892 USA. Univ Amsterdam, Dept Cell Biol & Histol, Amsterdam, Netherlands. RI Leitner, Wolfgang/F-5741-2011 OI Leitner, Wolfgang/0000-0003-3125-5922 NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 724 BP S121 EP S121 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800720 ER PT J AU Nograles, KE Miyagawa, F Katz, SI AF Nograles, K. E. Miyagawa, F. Katz, S. I. TI CD4+regulatory T cells maintain peripheral tolerance and survival in double transgenic (K14sOVA x OT- I) mice SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, NCI, Dermatol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 31 BP S6 EP S6 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800032 ER PT J AU Oh, KS Kim, YS Bustin, M Kraemer, KH AF Oh, K. S. Kim, Y. S. Bustin, M. Kraemer, K. H. TI Influence of XPB helicase on post-UV recruitment and redistribution of nucleotide excision repair proteins and histone H2AX and ATM phosphorylation in XP-B cells SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Natl Canc Inst, Basic Res Lab, Bethesda, MD USA. Natl Canc Inst, Lab Metab, Bethesda, MD USA. RI Bustin, Michael/G-6155-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 842 BP S141 EP S141 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800839 ER PT J AU Park, H Li, S Trempus, C Cotsarelis, G Morris, RJ AF Park, H. Li, S. Trempus, C. Cotsarelis, G. Morris, R. J. TI Skin tumor formation from hair follicle bulge keratinocytes in Krt1-15CrePR1;R26R mice SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Columbia Univ Coll Phys & Surg, Dept Dermatol, New York, NY 10032 USA. NIEHS, Canc Biol Grp, Res Triangle Pk, NC 27709 USA. Univ Penn, Sch Med, Dept Dermatol, Philadelphia, PA 19104 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 163 BP S28 EP S28 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800163 ER PT J AU Pietroni, V Rivera, Y Radoja, N Stimpson, S Anderson, DE Blumenberg, M Morasso, M AF Pietroni, V. Rivera, Y. Radoja, N. Stimpson, S. Anderson, D. E. Blumenberg, M. Morasso, M. TI Role of Dlx3 in the regulation of cell cycle proteins SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, NIAMS, Dev Skin Biol Unit, Bethesda, MD 20892 USA. NIH, NIDDK, Proteom & Mass Spectrometry Facility, Bethesda, MD 20892 USA. NYU, Dept Dermatol, New York, NY 10016 USA. NYU, Dept Biochem, New York, NY 10016 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 491 BP S82 EP S82 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800485 ER PT J AU Rao, T DiGiovanna, JJ Tamura, D Ueda, T Boyle, J Nadem, C Oh, K Khan, S Patronas, N Schiffmann, R Brooks, BP Kraemer, KH AF Rao, T. DiGiovanna, J. J. Tamura, D. Ueda, T. Boyle, J. Nadem, C. Oh, K. Khan, S. Patronas, N. Schiffmann, R. Brooks, B. P. Kraemer, K. H. TI Features of both xeroderma pigmentosum and trichothiodystrophy presenting in patients with mutations in the XPD gene SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, Bethesda, MD 20892 USA. NIH, CTRP Fellow, Bethesda, MD 20892 USA. Brown Med Sch, Providence, RI USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 630 BP S105 EP S105 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800625 ER PT J AU Scharschmidt, T List, K Szabo, R Bugge, T Segre, J AF Scharschmidt, T. List, K. Szabo, R. Bugge, T. Segre, J. TI Aberrant filaggrin processing in matriptase deficient mice: Potential model for atopic dermatitis? SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, NHGRI, Genet & Mol Biol Branch, Bethesda, MD 20892 USA. NIH, NIDCR, Oral & Pharyngeal Canc Branch, Bethesda, MD 20892 USA. HHMI, Res Scholars Program, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 566 BP S95 EP S95 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800564 ER PT J AU Suh, KS Mutoh, M Mutoh, T Li, L Ryscavage, A Crutchley, J Dumont, R Cheng, C Yuspa, SH AF Suh, K. S. Mutoh, M. Mutoh, T. Li, L. Ryscavage, A. Crutchley, J. Dumont, R. Cheng, C. Yuspa, S. H. TI CLIC4 mediates and is required for calcium induced keratinocyte differentiation SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, Lab Canc Biol & Genet, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 434 BP S73 EP S73 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800433 ER PT J AU Takeuchi, S Takeuchi, F Furue, M Katz, SI AF Takeuchi, S. Takeuchi, F. Furue, M. Katz, S. I. TI Dermal infiltration of monocytes, but not neutrophils, is required for the generation of contact hypersensitivity SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Kyushu Univ, Grad Sch Med Sci, Fukuoka 812, Japan. NCI, NIH, Dermatol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 736 BP S123 EP S123 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800732 ER PT J AU Tang, X Kim, AL Kopelovich, L Bickers, DR Athar, M AF Tang, X. Kim, A. L. Kopelovich, L. Bickers, D. R. Athar, M. TI Use of genetically engineered murine models to assess the chemoprevention of nonmelanoma skin cancer (NMSC) by alpha-difluoromethylornithine (DFMO) SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Columbia Univ, New York, NY USA. NCI, Div Canc Prevent, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 129 BP S22 EP S22 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800129 ER PT J AU Terunuma, A Patel, G Blonder, J Kapoor, V Telford, W Veenstra, T Vogel, J AF Terunuma, A. Patel, G. Blonder, J. Kapoor, V. Telford, W. Veenstra, T. Vogel, J. TI Proteomic characterization of molecular signatures for keratinocyte stem cells, transit amplifying keratinocytes and melanocytes in human epidermis SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Natl Canc Inst, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 475 BP S80 EP S80 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800472 ER PT J AU Therrien, J Terunuma, A Tock, CL Pfuztner, W Ohyama, M Vogel, JC AF Therrien, J. Terunuma, A. Tock, C. L. Pfuztner, W. Ohyama, M. Vogel, J. C. TI Skin gene therapy: systemic delivery of the anti-hypertensive atrial natriuretic peptide (ANP) by genetically-modified human skin equivalents (HSE) SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, Dermatol Branch, NIH, Bethesda, MD 20892 USA. Keio Univ, Sch Med, Dept Dermatol, Tokyo, Japan. Univ Marburg, Dept Dermatol, Marburg, Germany. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 541 BP S91 EP S91 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800537 ER PT J AU Wang, J Li, S Fan, Q Pacheco-Rodriguez, G Moss, J Darling, TN AF Wang, J. Li, S. Fan, Q. Pacheco-Rodriguez, G. Moss, J. Darling, T. N. TI Independent origins for multifocal skin tumors in tuberous sclerosis complex SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA. Natl Heart Lung & Blood Inst, Pulmonary Critical Care Med Branch, Bethesda, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 137 BP S23 EP S23 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800136 ER PT J AU Wang, Y DiGiovanna, J Stern, JB Hornyak, T Raffeld, M Kraemer, KH AF Wang, Y. DiGiovanna, J. Stern, J. B. Hornyak, T. Raffeld, M. Kraemer, K. H. TI UV signature mutations in melanomas from xeroderma pigmentosum patients SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NIH, Bethesda, MD 20892 USA. Brown Univ, Sch Med, Providence, RI 02912 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 903 BP S151 EP S151 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800897 ER PT J AU Wolf, R Dong, H Voscopoulos, C Cataisson, C Mascia, F Winston, J Goldsmith, P FitzGerald, P Howard, Z Yuspa, SH AF Wolf, R. Dong, H. Voscopoulos, C. Cataisson, C. Mascia, F. Winston, J. Goldsmith, P. FitzGerald, P. Howard, Z. Yuspa, S. H. TI The highly similar S100A7/S100A15 paralogs are differentially expressed in normal skin and inflamed psoriasis and function as chemotactic proteins SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 65 BP S11 EP S11 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800064 ER PT J AU Wu, A Zhu, Y Tang, X Kim, AL Kopelovich, L Bickers, DR Athar, M AF Wu, A. Zhu, Y. Tang, X. Kim, A. L. Kopelovich, L. Bickers, D. R. Athar, M. TI Cyclooxygenase-2 (COX-2) inhibition by nimesulide blocks ultraviolet-B (UVB)-induced photocarcinogenesis in mouse skin SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Columbia Univ, New York, NY USA. NCI, Div Canc Prevent, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 147 BP S25 EP S25 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800146 ER PT J AU Yamaguchi, Y Passeron, T Katayama, I Hearing, VJ AF Yamaguchi, Y. Passeron, T. Katayama, I. Hearing, V. J. TI The effects of dickkopf 1 on gene expression and Wnt signaling by keratinocytes: Mechanisms regulating skin pigmentation and thickness SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 NCI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. Osaka Univ, Dept Dermatol, Suita, Osaka, Japan. RI Yamaguchi, Yuji/B-9312-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 871 BP S146 EP S146 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800866 ER PT J AU Zhang, L Tang, X Kim, AL Kopelovich, L Bickers, DR Athar, M AF Zhang, L. Tang, X. Kim, A. L. Kopelovich, L. Bickers, D. R. Athar, M. TI Ptc1+/-/SKH-1 hairless mice developed rhabdomyosarcomas spontaneously with high-penetrance SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract CT 68th Annual Meeting of the Society-for-Investigative-Dermatology CY MAY 09-12, 2007 CL Los Angeles, CA SP Soc Investigat Dermatol C1 Columbia Univ, New York, NY USA. NCI, Div Canc Prevent, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 2007 VL 127 SU 1 MA 128 BP S22 EP S22 PG 1 WC Dermatology SC Dermatology GA 152UP UT WOS:000245387800131 ER PT J AU Lazarova, N Simeon, FG Musachio, JL Lu, SY Pike, VW AF Lazarova, Neva Simeon, Fabrice G. Musachio, John L. Lu, Shuiyu Pike, Victor W. TI Integration of a microwave reactor with Synthia to provide a fully automated radiofluorination module SO JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS LA English DT Article; Proceedings Paper CT 9th International Symposium on Synthesis and Application of Isotopes and Isotopically Labelled Compounds CY JUL 16-20, 2006 CL Edinburgh, SCOTLAND DE microwaves; Synthia; fluorine-18; m-fluorination; radioligand C1 NIMH, NIH, Mol Imaging Branch, Bethesda, MD 20892 USA. RP Lu, SY (reprint author), NIMH, NIH, Mol Imaging Branch, Bldg 10,Room B3C346, Bethesda, MD 20892 USA. EM shuiyu.lu@mail.nih.gov OI Lu, Shuiyu/0000-0003-0310-4318 NR 7 TC 23 Z9 23 U1 0 U2 2 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0362-4803 J9 J LABELLED COMPD RAD JI J. Label. Compd. Radiopharm. PD APR-MAY PY 2007 VL 50 IS 5-6 BP 463 EP 465 DI 10.1002/jlcr.1196 PG 3 WC Biochemical Research Methods; Chemistry, Medicinal; Chemistry, Analytical SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Chemistry GA 206YK UT WOS:000249218600062 ER PT J AU Lu, S Clements, JT Gilde, MJ Prak, A Watts, P Pike, VW AF Lu, Shuiyu Clements, James T. Gilde, Melis Jan Prak, Albert Watts, Paul Pike, Victor W. TI Separation of (F-18)fluoride ion from proton-irradiated (O-18)water within an EOF-driven micro-reactor powered by the Capilix Capella (TM) platform SO JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS LA English DT Article; Proceedings Paper CT 9th International Symposium on Synthesis and Application of Isotopes and Isotopically Labelled Compounds CY JUL 16-20, 2006 CL Edinburgh, SCOTLAND DE micro-reactor; electroosmotic flow; PET; radiolabeling; [F-18]fluoride ion C1 NIMH, Mol Imaging Branch, NIH, Bethesda, MD 20892 USA. NanoSci Inc, Aliso Viejo, CA 92656 USA. Capilix BV, NL-7522 NM Enschede, Netherlands. Univ Hull, Dept Chem, Kingston Upon Hull HU6 7XR, N Humberside, England. RP Lu, SY (reprint author), NIMH, Mol Imaging Branch, NIH, Bldg 10,Room B3C346,10 Ctr Dr, Bethesda, MD 20892 USA. EM shuivu.lu@mail.nih.gov OI Lu, Shuiyu/0000-0003-0310-4318 NR 6 TC 6 Z9 6 U1 0 U2 3 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0362-4803 J9 J LABELLED COMPD RAD JI J. Label. Compd. Radiopharm. PD APR-MAY PY 2007 VL 50 IS 5-6 BP 597 EP 599 DI 10.1002/jlcr.1296 PG 3 WC Biochemical Research Methods; Chemistry, Medicinal; Chemistry, Analytical SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Chemistry GA 206YK UT WOS:000249218600121 ER PT J AU Rosenberg, HF Taylor, JJ AF Rosenberg, Helene F. Taylor, John J. TI Vasoactive intestinal peptide, periodontal disease, and the innate immune response: an interview with Dr. John J. Taylor SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Editorial Material ID PORPHYROMONAS-GINGIVALIS LIPOPOLYSACCHARIDE; EXPRESSION; VIP; TOLL-LIKE-RECEPTOR-4; INHIBITION; MODULATION C1 NIAID, Lab Allerg Dis, NIH, Bethesda, MD 20892 USA. RP Rosenberg, HF (reprint author), NIAID, Lab Allerg Dis, NIH, Bethesda, MD 20892 USA. EM hrosenberg@niaid.nih.gov NR 14 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PD APR PY 2007 VL 81 IS 4 BP 904 EP 906 DI 10.1189/jlb.1306086 PG 3 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA 153TL UT WOS:000245458000006 ER PT J AU Padigel, UM Stein, L Redding, K Lee, JJ Nolan, TJ Schad, GA Birnbaumer, L Abraham, D AF Padigel, Udaikumar M. Stein, Louis Redding, Kevin Lee, James J. Nolan, Thomas J. Schad, Gerhard A. Birnbaumer, Lutz Abraham, David TI Signaling through G alpha i2 protein is required for recruitment of neutrophils for antibody-mediated elimination of larval Strongyloides stercoralis in mice SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Article DE parasite; heterotrimeric; G protein; cytokine; antibody ID PERTUSSIS TOXIN; PROTECTIVE IMMUNITY; SCHISTOSOMA-MANSONI; CYTOKINE PRODUCTION; AUTOIMMUNE-DISEASE; ADAPTIVE IMMUNITY; BALB/CBYJ MICE; RESPONSES; ACTIVATION; INDUCTION AB The heterotrimeric guanine nucleotide-binding protein G alpha i2 is involved in regulation of immune responses against microbial and nonmicrobial stimuli. G alpha i2(-/-) mice have a selectively impaired IgM response consistent with a disorder in B cell development yet have augmented T cell effector function associated with increased production of IFN-gamma and IL-4. The goal of the present study was to determine if a deficiency in the G alpha i2 protein in mice would affect the protective immune response against Strongyloides stercoralis, which is IL-4-, IL-5-, and IgM-dependent. G alpha i2(-/-) and wild-type mice were immunized and challenged with S. stercoralis larvae and analyzed for protective immune responses against infection. G alpha i2(-/-) mice failed to kill the larvae in the challenge infection as compared with wild-type mice despite developing an antigen-specific Th2 response characterized by increased IL-4, IL-5, IgM, and IgG. Transfer of serum collected from immunized G alpha i2(-/-) mice to naive wild-type mice conferred passive protective immunity against S. stercoralis infection thus confirming the development of a protective antibody response in G alpha i2(-/-) mice. Differential cell analyses and mycloperoxidase assays for quantification of neutrophils showed a significantly reduced recruitment of nentrophils into the microenvironment of the parasites in immunized G alpha i2(-/-) mice. However, cell transfer studies demonstrated that nentrophils from G alpha i2(-/-) mice are competent in killing larvae. These data demonstrate that G alpha i2 signaling events are not required for the development of the protective immune responses against S. stercoralis; however, G alpha i2 is essential for the recruitment of neutrophils required for host-dependent killing of larvae. C1 Thomas Jefferson Univ, Dept Microbiol & Immunol, Philadelphia, PA 19107 USA. Mayo Clin, Dept Biochem & Mol Biol, Scottsdale, AZ USA. Univ Penn, Dept Pathobiol, Philadelphia, PA 19104 USA. NIEHS, Lab Signal Transduct, Res Triangle Pk, NC 27709 USA. RP Abraham, D (reprint author), Thomas Jefferson Univ, Dept Microbiol & Immunol, 223 S 10th St, Philadelphia, PA 19107 USA. EM david.abraham@jefferson.edu FU Intramural NIH HHS [Z01 ES101643-05]; NIAID NIH HHS [R01 AI 22662, R01 AI047189, R01 AI47189] NR 39 TC 14 Z9 14 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PD APR PY 2007 VL 81 IS 4 BP 1120 EP 1126 DI 10.1189/jlb.1106695 PG 7 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA 153TL UT WOS:000245458000030 PM 17242370 ER PT J AU Ma, KZ Langenbach, R Rapoport, SI Basselin, M AF Ma, Kaizong Langenbach, Robert Rapoport, Stanley I. Basselin, Mireille TI Altered brain lipid composition in cyclooxygenase-2 knockout mouse SO JOURNAL OF LIPID RESEARCH LA English DT Article DE fatty acids; cholesterol; phosphatidylserine ID FOCUSED MICROWAVE IRRADIATION; PLASMA-MEMBRANE SPHINGOMYELIN; THIN-LAYER CHROMATOGRAPHY; FATTY-ACID COMPOSITION; ARACHIDONIC-ACID; RAT-BRAIN; TRANSPHOSPHATIDYLATED PHOSPHATIDYLSERINE; PHOSPHOLIPID-METABOLISM; QUANTITATIVE ANALYSIS; DOCOSAHEXAENOIC ACID AB Cyclooxygenase (COX)-2 plays an important role in brain arachidonic acid (20:4n-6) metabolism, and its expression is upregulated in animal models of neuroinflammation and excitotoxicity. Our hypothesis was that brain lipid composition would be altered in COX-2 knockout (COX-2(-/-)) compared with wild-type (COX-2(+/+)) mice, reflecting the important role of COX-2 in brain lipid metabolism. Concentrations of different lipids were measured in high-energy microwaved brain from COX-2(-/-) and COX-2(+/+) mice. Compared with the COX-2(+/+) mouse brain, the brain of the COX-2(-/-) mouse had a statistically significant 15% increase in phosphatidylserine (PtdSer) and significant 37, 27, and 32% reductions in triacylglycerol and cholesterol concentrations and in the cholesterol-to-phospholipid ratio, respectively. ne normalized concentration of palmitic acid (16:0) was increased in PtdSer, as was the brain concentration of unesterified arachidic acid (20:0). A lifetime absence of COX-2 produces multiple changes in brain lipid composition. These changes may be related to reported changes in fatty acid kinetics and in resistance to neuroinflammation and excitotoxicity in the COX-2(-/-) mouse., C1 NIA, Brain Physiol & Metab Sect, NIH, Bethesda, MD 20892 USA. Natl Inst Environm Hlth Sci, NIH, Res Triangle Pk, NC 27709 USA. RP Basselin, M (reprint author), NIA, Brain Physiol & Metab Sect, NIH, Bethesda, MD 20892 USA. EM mirvasln@mail.nih.gov FU Intramural NIH HHS NR 66 TC 17 Z9 18 U1 0 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0022-2275 J9 J LIPID RES JI J. Lipid Res. PD APR PY 2007 VL 48 IS 4 BP 848 EP 854 DI 10.1194/jlr.M600400-JLR200 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 156DA UT WOS:000245627100010 PM 17202128 ER PT J AU Pawlosky, RJ Hibbeln, JR Salem, N AF Pawlosky, Robert J. Hibbeln, Joseph R. Salem, Norman, Jr. TI Compartmental analyses of plasma n-3 essential fatty acids among male and female smokers and nonsmokers SO JOURNAL OF LIPID RESEARCH LA English DT Article DE linolenic acid; docosahexaenoic acid; isotope tracer; mass spectrometry; humans; kinetics; controlled diet ID ALPHA-LINOLENIC ACID; SMOKING; METABOLISM; CONVERSION; PRODUCTS; WOMEN AB The effects of cigarette smoking on n-3 essential FA metabolism were studied in male and female subjects by fitting the concentration-time curves of the d(5)-labeled plasma fatty acids (FAs) originating from a dose of d(5)-18:3n-3 to a compartmental model of n-3 FA metabolism. For 3 weeks, female (smokers, n = 5; nonsmokers, n = 5) and male (smokers, n = 5; nonsmokers, n = 5) subjects subsisted on a beef-based diet. Beginning in the third week, subjects received a dose of d(5)-18:3n-3 ethyl ester (I g). Plasma FAs were analyzed using gas chromatography (GC) and GC-mass spectrometry, and the kinetic rate parameters were determined from the concentration-time curves for d(5)-18:3n-3, d(5)-20:5n-3, d(5)-22:5n-3, and d(5)-22:6n-3. Women smokers had a 2-fold greater percent of dose in plasma (5.8% vs. 2.9%; P < 0.01) and a higher fractional rate constant coefficient for formation of d5-22:6n-3 from d(5)-22:5n-3 (0.03 h(-1) vs. 0.01 h(-1); P < 0.01), compared with nonsmokers. Male smokers had elevated total plasma n-3 FAs, more-rapid turnover of 18:3n-3 (13.3 mg/day(-1) vs. 4.3 mg/day(-1); P < 0.001), a disappearance rate of d(5)-20:5n-3 that was both delayed and slower (0.001 h(-1) vs. 0.012 h(-1); P < 0.05), and a percentage of d(5)-20:5n-3 directed into formation of d(5)-22:5n-3 (99% vs. 61%; P < 0.03) that was greater compared with nonsmokers. Smoking increased the bioavailability of n-3 FAs from plasma, accelerated the fractional synthetic rates, and heightened the percent formation of some long-chain n-3 PUFAs in men and women. C1 NIAAA, Lab Metab Control, NIH, Bethesda, MD USA. NIAAA, Lab Membrane Biochem & Biophys, NIH, Bethesda, MD USA. RP Pawlosky, RJ (reprint author), NIAAA, Lab Metab Control, NIH, Bethesda, MD USA. EM bpawl@mail.nih.gov NR 16 TC 39 Z9 41 U1 1 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0022-2275 J9 J LIPID RES JI J. Lipid Res. PD APR PY 2007 VL 48 IS 4 BP 935 EP 943 DI 10.1194/jlr.M600310-JLR200 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 156DA UT WOS:000245627100019 PM 17234605 ER PT J AU Hu, JN Yang, SL Xuan, Y Jiang, Q Yang, YH Haacke, EM AF Hu, Jiani Yang, Shaolin Xuan, Yang Jiang, Quart Yang, Yihong Haacke, E. Mark TI Simultaneous detection of resolved glutamate, glutamine, and gamma-aminobutyric acid at 4 T SO JOURNAL OF MAGNETIC RESONANCE LA English DT Article DE glutamate; glutamine; gamma-aminobutyric acid (GABA); H-1 MR spectroscopy; STEAM ID MAGNETIC-RESONANCE SPECTROSCOPY; MULTIPLE-QUANTUM FILTER; HUMAN BRAIN; IN-VIVO; MR SPECTROSCOPY; COUPLED SPINS; 3 TESLA; GABA; PRESS; QUANTIFICATION AB A new approach is introduced to simultaneously detect resolved glutamate (Gin), glutamine (Gin), and gamma-aminobutyric acid (GABA) using a standard STEAM localization pulse sequence with the optimized sequence timing parameters. This approach exploits the dependence of the STEAM spectra of the strongly coupled spin systems of Gin, Gin, and GABA on the echo time TE and the mixing time TM at 4 T to find an optimized sequence parameter set, i.e., {TE, TM}, where the outer-wings of the Gin C4 multiplet resonances around 2.35 ppm, the Gin C4 multiplet resonances around 2.45 ppm, and the GABA C2 multiplet resonance around 2.28 ppm are significantly suppressed and the three resonances become virtual singlets simultaneously and thus resolved. Spectral simulation and optimization were conducted to find the optimized sequence parameters, and phantom and in vivo experiments (on normal human brains, one patient with traumatic brain injury, and one patient with brain tumor) were carried out for verification. The results have demonstrated that the Gin, Glu, and GABA signals at 2.2-2.5 ppm can be well resolved using a standard STEAM sequence with the optimized sequence timing parameters around {82 ms, 48 ms} at 4 T, while the other main metabolites, such as N-acetyl aspartate (NAA), choline (Who), and creatine (tCr), are still preserved in the same spectrum. The technique can be easily implemented and should prove to be a useful tool for the basic and clinical studies associated with metabolism of Gin, Gin, and/or GABA. (C) 2006 Elsevier Inc. All rights reserved. C1 Wayne State Univ, Dept Radiol, Detroit, MI 48201 USA. Natl Inst Drug Abuse, Neuroimaging Res Branch, Baltimore, MD 21224 USA. Henry Ford Hosp, Dept Neurol, Detroit, MI 48201 USA. RP Hu, JN (reprint author), Wayne State Univ, Dept Radiol, Detroit, MI 48201 USA. EM jhu@med.wayne.edu FU Intramural NIH HHS [Z01 DA000469-02] NR 40 TC 22 Z9 23 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1090-7807 J9 J MAGN RESON JI J. Magn. Reson. PD APR PY 2007 VL 185 IS 2 BP 204 EP 213 DI 10.1016/j.jmr.2006.12.010 PG 10 WC Biochemical Research Methods; Physics, Atomic, Molecular & Chemical; Spectroscopy SC Biochemistry & Molecular Biology; Physics; Spectroscopy GA 176HT UT WOS:000247076200003 PM 17223596 ER PT J AU Koyama, Y Talanov, VS Bernardo, M Hama, Y Regino, CAS Brechbiel, MW Choyke, PL Kobayashi, H AF Koyama, Yoshinori Talanov, Vladimir S. Bernardo, Marcelino Hama, Yukihiro Regino, Celeste A. S. Brechbiel, Martin W. Choyke, Peter L. Kobayashi, Hisataka TI A dendrimer-based nanosized contrast agent, dual-labeled for magnetic resonance and optical fluorescence imaging to localize the sentinel lymph node in mice SO JOURNAL OF MAGNETIC RESONANCE IMAGING LA English DT Article DE sentinel lymph node; MRI; near infrared optical; imaging; hybrid probe; macromolecular contrast agent; nanotechnology ID EARLY BREAST-CANCER; BIOPSY; LYMPHANGIOGRAPHY; VISUALIZATION; MELANOMA AB Purpose: To preoperatively and intraoperatively localize the sentinel lymph node (SLN), a single hybrid probe for MR and near infrared (NIR) optical imaging was synthesized and tested. Materials and Methods: A macromolecular MR/NIR optical contrast agent was synthesized based on a similar to 191 gadolinium-labeled contrast agent using generation-6 polyamidoamine dendrimer (G6), which is also labeled with 2 Cy5.5, an NIR fluorophore. After establishing the optimal dose, the agent was injected into mammary glands of 10 normal mice to examine the lymphatic drainage from the breast using a 3T clinical scanner. Immediately after the MRI scan, NIR optical imaging and image-guided surgery were performed to compare the two imaging modalities. Results: To consistently identify the SLNs, we needed to inject 25 mu L of 30 mM [Gd] G6-Cy5.5. All SLNs could be easily identified and resected under NIR optical imaging-guided surgery. Although external NIR optical imaging failed to identify SLNs close to the injection site due to shinethrough, MR lymphography (MRL) consistently identified all SLNs regardless of their location. Conclusion: We have successfully synthesized and tested a dual labeled MR/NIR optical hybrid contrast agent, G6-Cy5.5 for reoperative and intraoperative localization of SLNs. C1 NCI, Mol Imaging Program, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. NCI, Radiat Oncol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. SAIC Frederick, Res Technol Program, Frederick, MD USA. RP Kobayashi, H (reprint author), NCI, Mol Imaging Program, Ctr Canc Res, NIH, Bldg 10,Rm 1B40,MSC1088,10 Ctr Dr, Bethesda, MD 20892 USA. EM Kobayash@mail.nih.gov NR 18 TC 96 Z9 101 U1 3 U2 27 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 1053-1807 J9 J MAGN RESON IMAGING JI J. Magn. Reson. Imaging PD APR PY 2007 VL 25 IS 4 BP 866 EP 871 DI 10.1002/jmri.20852 PG 6 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 156FK UT WOS:000245633300026 PM 17345640 ER PT J AU Jobsis, PD Rothstein, EC Balaban, RS AF Jobsis, Paul D. Rothstein, Emily C. Balaban, Robert S. TI Limited utility of acetoxymethyl (AM)-based intracellular delivery systems, in vivo: interference by extracellular esterases SO JOURNAL OF MICROSCOPY-OXFORD LA English DT Article DE extracellular space; INDO; microscopy; mouse; nuclei; rabbit; skeletal muscle; SNARF; spectroscopy; two-photon; vascular imaging ID RABBIT HEARTS; CALCIUM; FLUORESCENCE; MICROSCOPY; PH; TRANSPORTER; TRANSIENTS; INDICATOR; ANIMALS; RELEASE AB The use of acetoxymethyl (AM) groups to deliver and trap exogenous optical probes inside cells is an established tool in cell biology/physiology, however, these probes have not been used extensively in vivo. In this study, the use of the acetoxymethyl delivery system for optical probes was evaluated, in vivo. Initial studies revealed very little trapped probe in intact tissues even when near saturating levels of probe were injected in living animals. We tested the hypothesis that extracellular esterases rapidly cleave the acetoxymethyl groups preventing the probes from entering cells, in vivo. The rates of hydrolysis of 11 acetoxymethyl probes in diluted porcine plasma revealed an essentially first order high rate dye cleavage with half times on the order of minutes or less. Studies on mice and rabbits revealed rates 10- to 2-fold higher, respectively. These plasma studies suggested that the acetoxymethyl probes were being cleaved before having a chance to enter cells in tissues in vivo. This was confirmed using intravital 2-photon excitation microscopy in muscle tissue where several acetoxymethyl probes were found to rapidly cleave in the vascular space during infusion and not be trapped in the muscle cells. Studies with succinimidyl esters that should quickly bind to proteins on cleavage also failed to enter cells, in vivo, consistent with the notion that the cleavage was occurring in the extracellular space. These data suggest that the high level of plasma and extracellular esterase activity render the classical acetoxymethyl probes ineffective for monitoring intracellular events, in vivo. Different approaches to trapping exogenous probes will need to be explored for physiological studies using intravital microscopy. C1 NHLBI, Cardiac Energet Lab, Bethesda, MD 20892 USA. RP Balaban, RS (reprint author), Eli Lilly & Co, Ctr Mol & Anat Imaging, POB 708, Greenfield, IN 46140 USA. EM rsb@nih.gov FU Intramural NIH HHS [Z01 HL004601-18] NR 26 TC 20 Z9 20 U1 2 U2 6 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0022-2720 J9 J MICROSC-OXFORD JI J. Microsc.-Oxf. PD APR PY 2007 VL 226 IS 1 BP 74 EP 81 PG 8 WC Microscopy SC Microscopy GA 148UK UT WOS:000245101000010 PM 17381712 ER PT J AU Nikolic, I Liu, DX Bell, JA Collins, J Steenbergen, C Murphy, E AF Nikolic, Ivana Liu, Dianxin Bell, Jamie A. Collins, Jennifer Steenbergen, Charles Murphy, Elizabeth TI Treatment with an estrogen receptor-beta-selective agonist is cardioprotective SO JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY LA English DT Article DE ischemia; estrogen; heart; cardioprotection ID ISCHEMIA-REPERFUSION INJURY; ISCHEMIA/REPERFUSION INJURY; ER-BETA; TRANSGENIC MICE; UP-REGULATION; RABBIT HEART; EXPRESSION; ALPHA; SUSCEPTIBILITY; PROTEIN AB This study was designed to investigate whether treatment with an estrogen receptor-beta (ER-beta)-selective agonist (2,3-bis(4-hydroxyphenyl)-propionitrile, DPN) can provide cardioprotection in female mice lacking endogenous estrogen. To study the effect of ER-beta stimulation in ischemia-reperfusion injury, we treated ovariectomized (ovx) female mice with 0.1 mg/kg/day of 17 beta-estradiol, 0.8 mg/kg/day of DPN, or vehicle for 2 weeks. Isolated hearts were Langendorff perfused for 25 min prior to a 1-min treatment with isoproterenol, followed by 20 min of nonnothermic global ischemia and 40 min of reperfusion. Left ventricular developed pressure (LVDP) and heart rate were measured. Recovery of function at the end of 40 min of reperfusion was expressed as a percentage of pre-ischemic rate pressure product (RPP=LVDP x heart rate). Hearts from ovx female mice had a significantly lower recovery of LVDP than the hearts from intact female mice (12.4 +/- 1.6% vs. 19.6 +/- 1.6%, p < 0.05, respectively). Furthermore, hearts from ovx female mice treated with DPN exhibited significantly better functional recovery than hearts from either vehicle-treated ovx female mice (20.1 +/- 2.2% vs. 12.4 +/- 1.6%, p < 0.05, respectively) or wild type male mice (20.1 +/- 2.2% vs. 6.4 +/- 0.6%, p < 0.05, respectively). DPN did not increase uterine weight in ovx females compared to vehicle treatment. Gene profiling showed that treatment with DPN resulted in upregulation of a number of protective genes such as heat shock protein 70, the antiapoptotic protein, growth arrest and DNA damage 45 beta, and cyclooxygenase 2. Published by Elsevier Inc. C1 NIEHS, Lab Signal Transduct, NIH, DHHS, Durham, NC USA. NIEHS, Microarray Ctr, NIH, DHHS, Durham, NC USA. Duke Univ, Sch Med, Dept Pathol, Durham, NC 27706 USA. NHLBI, Vasc Med Branch, NIH, DHHS, Bethesda, MD 20892 USA. RP Murphy, E (reprint author), NIEHS, Lab Signal Transduct, NIH, DHHS, Durham, NC USA. EM murphy1@niehs.nih.gov FU Intramural NIH HHS; NHLBI NIH HHS [HL39752, R01 HL039752] NR 35 TC 59 Z9 62 U1 0 U2 3 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2828 EI 1095-8584 J9 J MOL CELL CARDIOL JI J. Mol. Cell. Cardiol. PD APR PY 2007 VL 42 IS 4 BP 769 EP 780 DI 10.1016/j.yjmcc.2007.01.014 PG 12 WC Cardiac & Cardiovascular Systems; Cell Biology SC Cardiovascular System & Cardiology; Cell Biology GA 165EU UT WOS:000246287600010 PM 17362982 ER PT J AU Gil, M McKinney, C Lee, MK Eells, JB Phyillaier, MA Nikodem, VM AF Gil, Minchan McKinney, Cushla Lee, Mi Kyeong Eells, Jeffrey B. Phyillaier, Marcia A. Nikodem, Vera M. TI Regulation of GTP cyclohydrolase I expression by orphan receptor Nurr1 in cell culture and in vivo SO JOURNAL OF NEUROCHEMISTRY LA English DT Article DE GTP-cyclohydrolase I; Nurr1; tetrahydrobiopterin ID TYROSINE-HYDROXYLASE GENE; MESSENGER-RNA EXPRESSION; NUCLEAR RECEPTOR; NEURON DEVELOPMENT; HETEROZYGOUS MICE; DOPAMINE NEURONS; HPH-1 MOUSE; TETRAHYDROBIOPTERIN; METABOLISM; BRAIN AB Nurr1 is an orphan nuclear transcription factor essential for the terminal differentiation of dopamine (DA) neurons in the ventral midbrain (VM). To identify the Nurr1-target genes, we carried out microarray and quantitative real-time PCR analyses of Nurr1 null and wild-type mice in VM at embryonic day (E) 12.5 and shortly after birth (P0). In addition to the absence of mRNAs of DA synthesizing enzymes, the guanosine 5'-triphosphate (GTP) cyclohydrolase I (GTPCH) was also substantially reduced in the VM of Nurr1-null mice. GTPCH is the first enzyme in the synthesis pathway of tetrahydrobiopterin (BH4), an essential cofactor for tyrosine hydroxylase in DA synthesis. In the mouse, Nurr1 and GTPCH mRNA were first detected at E10.5, and GTPCH transcription paralleled that of Nurr1. Small interfering RNA targeted against Nurr1 decreases GTPCH expression in MC3T3-E1 osteoblasts in cell culture. Cotransfection of Nurr1 and the GTPCH-luciferase (luc) reporter increased the luc activity by about threefold in N2A cells. Additional analysis using 5'-deletions and mutants revealed that Nurr1 activates GTPCH transcription indirectly through the proximal promoter region, in the absence of the nerve growth factor-induced clone B (NGFI-B) responsive element-like sites, similarly, as recently reported for DA transporter regulation by Nurr1. C1 NIDDKD, Genet & Biochem Branch, NIH, Bethesda, MD 20892 USA. RP Nikodem, VM (reprint author), NIDDKD, Genet & Biochem Branch, NIH, Bldg 8-106,9000 Rockville Pike, Bethesda, MD 20892 USA. EM veran@intra.niddk.nih.gov RI McKinney, Cushla/A-7407-2012; OI Eells, Jeffrey/0000-0002-6381-1666 FU Intramural NIH HHS NR 47 TC 14 Z9 18 U1 0 U2 2 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PD APR PY 2007 VL 101 IS 1 BP 142 EP 150 DI 10.1111/j.1471-4159.2006.04356.x PG 9 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA 145SU UT WOS:000244885600013 PM 17394463 ER PT J AU Borycz, J Pereira, MF Melani, A Rodrigues, RJ Kofalvi, A Panlilio, L Pedata, F Goldberg, SR Cunha, RA Ferre, S AF Borycz, Janusz Pereira, M. Fatima Melani, Alessia Rodrigues, Ricardo J. Kofalvi, Attila Panlilio, Leigh Pedata, Felicita Goldberg, Steven R. Cunha, Rodrigo A. Ferre, Sergi TI Differential glutamate-dependent and glutamate-independent adenosine A(1) receptor-mediated modulation of dopamine release in different striatal compartments SO JOURNAL OF NEUROCHEMISTRY LA English DT Article DE adenosine A(1) receptor; caffeine; dopamine; glutamate; rat; striatum ID METHYL-D-ASPARTATE; RAT NUCLEUS-ACCUMBENS; EXTRACELLULAR DOPAMINE; EVOKED DOPAMINE; SHELL; CAFFEINE; LOCALIZATION; BRAIN; A(2A); NEUROTRANSMISSION AB Adenosine and dopamine are two important modulators of glutamatergic neurotransmission in the striatum. However, conflicting reports exist about the role of adenosine and adenosine receptors in the modulation of striatal dopamine release. It has been previously suggested that adenosine A(1) receptors localized in glutamatergic nerve terminals indirectly modulate dopamine release, by their ability to modulate glutamate release. In the present study, using in vivo microdialysis, we provide evidence for the existence of a significant glutamate-independent tonic modulation of dopamine release in most of the analyzed striatal compartments. In the dorsal, but not in the ventral, part of the shell of the nucleus accumbens (NAc), blockade of A(1) receptors by local perfusion with the selective A(1) receptor antagonist 8-cyclopentyl-1,3-dimethyl-xanthine or by systemic administration of the non-selective adenosine antagonist caffeine induced a glutamate-dependent release of dopamine. On the contrary, A(1) receptor blockade induced a glutamate-independent dopamine release in the core of the NAc and the nucleus caudate-putamen. Furthermore, using immunocytochemical and functional studies in rat striatal synaptosomes, we demonstrate that a fraction of striatal dopaminergic terminals contains adenosine A(1) receptors, which directly inhibit dopamine release independently of glutamatergic transmission. C1 Natl Inst Drug Abuse, Intramural Res Program, Dept Hlth & Human Serv, NIH, Baltimore, MD 21224 USA. Univ Coimbra, Fac Med, Inst Biochem, Ctr Neurosci Coimbra, Coimbra, Portugal. Univ Florence, Dept Preclin & Clin Pharmacol, Florence, Italy. RP Ferre, S (reprint author), Natl Inst Drug Abuse, Intramural Res Program, Dept Hlth & Human Serv, NIH, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM sferre@intra.nida.nih.gov RI Ferre, Sergi/K-6115-2014; Rodrigues, Ricardo/B-3847-2008; Cunha, Rodrigo/E-7475-2015; OI Ferre, Sergi/0000-0002-1747-1779; Rodrigues, Ricardo/0000-0002-7631-743X; Cunha, Rodrigo/0000-0003-2550-6422; Kofalvi, Attila/0000-0001-6910-9707 FU Intramural NIH HHS NR 34 TC 48 Z9 49 U1 0 U2 6 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PD APR PY 2007 VL 101 IS 2 BP 355 EP 363 DI 10.1111/j.1471-4159.2006.04386.x PG 9 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA 151TD UT WOS:000245312800006 PM 17254024 ER PT J AU Rabadan-Diehl, C Martinez, A Volpi, S Subburaju, S Aguilera, G AF Rabadan-Diehl, C. Martinez, A. Volpi, S. Subburaju, S. Aguilera, G. TI Inhibition of vasopressin V1b receptor translation by upstream open reading frames in the 5 '-untranslated region SO JOURNAL OF NEUROENDOCRINOLOGY LA English DT Article DE vasopressin V1b receptor; translational control; 5 '-untranslated region; minicistron; in vitro translation ID MESSENGER-RNA; GENE-EXPRESSION; CHRONIC STRESS; RAT; INITIATION; CLONING; ADRENALECTOMY; INVOLVEMENT; EUKARYOTES; SECRETION AB The 5'-UTR of the vasopressin V1b receptor (V1bR) mRNA contains small open reading frames (ORF) located upstream (u) of the main ORF encoding the V1bR. The ability of the three proximal uORFs to be translated into peptides and their influence on V1bR translation was examined using fusion constructs of uORFs and V5 epitope, or ATG/ATA uORF mutations in the V1bR cDNA. In vitro translation and western blot analysis after transfection of uORF1-V5 or uORF2-V5 into cells revealed that uORF1 can be translated. As predicted by computer analysis, in vitro translation using a rabbit reticulocyte/canine microsome system, immunohistochemistry and western blot in membranes of transfected cells with uORF1-V5 revealed translocation of the uORF1 peptide into membrane fractions. In vitro translation of V1bR cDNA with mutations of the two uORFs proximal to the initiating methionine, uORFs 1 and 2 (Mut 1-2), or uORF2 (Mut 2) showed significantly increased translation of a 46 kDa band corresponding to the V1bR, compared with wild-type (WT) V1bR, an effect that was attenuated by cotranslation of uORF1-V5. Consistently, VP-induced inositol phosphate formation was higher in Chinese hamster ovay cells transfected with Mut 1-2 than with WT V1bR. Immunohistochemical and western blot analysis, using an antibody against uORF1, revealed peptide immunoreactivity in rat pituitary but not in liver. Pituitary uORF immunoreactivity increased following glucocorticoid administration. The present study shows that uORFs in the 5'-UTR of the V1bR mRNA inhibit V1bR translation, and suggests that translation of a 38-amino acid membrane peptide encoded by uORF1 exerts tonic inhibition of V1bR translation. C1 NICHD, Dev Endocrinol Branch, NIH, Sect Endocrine Physiol, Bethesda, MD 20892 USA. Inst Cajal, Dept Cell Biol & Neuroanat, Madrid, Spain. RP Aguilera, G (reprint author), NICHD, Dev Endocrinol Branch, NIH, Sect Endocrine Physiol, Bldg 10 Rm 10N262, Bethesda, MD 20892 USA. EM greti_aguilera@.nih.gov RI Martinez, Alfredo/A-3077-2013 OI Martinez, Alfredo/0000-0003-4882-4044 NR 33 TC 9 Z9 9 U1 0 U2 1 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0953-8194 J9 J NEUROENDOCRINOL JI J. Neuroendocrinol. PD APR PY 2007 VL 19 IS 4 BP 309 EP 319 DI 10.1111/j.1365-2826.2007.01533.x PG 11 WC Endocrinology & Metabolism; Neurosciences SC Endocrinology & Metabolism; Neurosciences & Neurology GA 143RC UT WOS:000244740400009 PM 17355321 ER PT J AU Lule, D Diekmann, V Anders, S Kassubek, J Kubler, A Ludolph, AC Birbaumer, N AF Lule, Dorothee Diekmann, Volker Anders, Silke Kassubek, Jan Kuebler, Andrea Ludolph, Albert C. Birbaumer, Niels TI Brain responses to emotional stimuli in patients with amyotrophic lateral sclerosis (ALS) SO JOURNAL OF NEUROLOGY LA English DT Article DE amyotrophic lateral sclerosis (ALS); social information processing; emotions; fMRI ID EVENT-RELATED FMRI; ACTIVATION; AMYGDALA; RECOGNITION; CORTICES; AROUSAL; MOTION; FACES AB Amyotrophic lateral sclerosis (ALS), a progressive motor neuron disease, affects movement and communication abilities and emotional processing. Subjective ratings of emotional stimuli depicting social interactions and facial expressions differed significantly between ALS patients and healthy controls in a previous study with a reduction of negative emotional valence (pleasantness) and lower subjective arousal (excitement) in ALS patients. In the present study, sixty similar emotional slides were presented to 13 ALS patients, 15 matched healthy controls and six tetraplegic patients. Subjective reports of valence and arousal as well as brain responses to the affective pictures using functional magnetic resonance imaging (fMRI) were measured. The picture series was presented twice with a 6-months interval to investigate effects of disease progression. ALS patients presented an increased brain response in the right supramarginal area and a reduced brain response in extrastriate visual areas at both measurements compared with healthy controls. Within the ALS patients' group a reduction of brain responses in the anterior insula at the follow-up was correlated with the subjective arousal. The reduced response in the anterior insula is tentatively interpreted as indicating reduced arousal during the course of the disease at the neural and behavioural level. The reduction of activity in extrastriate visual areas might be similarly interpreted. The increased brain response in the right supramarginal area of ALS patients might represent an altered sensitivity to social-emotional cues. C1 Univ Ulm, Dept Neurophysiol, D-89081 Ulm, Germany. Univ Ulm, Dept Neurol, D-89081 Ulm, Germany. Univ Tubingen, Inst Med Psychol & Behav Neurobiol, Tubingen, Germany. NINDS, NIH, Bethesda, MD 20892 USA. RP Lule, D (reprint author), Univ Ulm, Dept Neurophysiol, Albert Einstein Allee 47, D-89081 Ulm, Germany. EM dorothee.lule@um-uhn.de RI Kassubek, Jan/F-2774-2015; OI Kubler, Andrea/0000-0003-4876-0415 NR 39 TC 33 Z9 34 U1 0 U2 3 PU DR DIETRICH STEINKOPFF VERLAG PI DARMSTADT PA PO BOX 10 04 62, D-64204 DARMSTADT, GERMANY SN 0340-5354 J9 J NEUROL JI J. Neurol. PD APR PY 2007 VL 254 IS 4 BP 519 EP 527 DI 10.1007/s00415-006-0409-3 PG 9 WC Clinical Neurology SC Neurosciences & Neurology GA 165KG UT WOS:000246303800018 PM 17401515 ER PT J AU Litvan, I Halliday, G Hallett, M Goetz, CG Rocca, W Duyckaerts, C Ben-Shlomo, Y Dickson, DW Lang, AE Chesselet, MF Langston, WJ Di Monte, DA Gasser, T Hagg, T Hardy, J Jenner, P Melamed, E Myers, RH Parker, D Price, DL AF Litvan, Irene Halliday, Glenda Hallett, Mark Goetz, Christopher G. Rocca, Walter Duyckaerts, Charles Ben-Shlomo, Yoav Dickson, Dennis W. Lang, Anthony E. Chesselet, Marie-Francoise Langston, William J. Di Monte, Donato A. Gasser, Thomas Hagg, Theo Hardy, John Jenner, Peter Melamed, Eldad Myers, Richard H. Parker, Davis, Jr. Price, Donald L. TI The etiopathogenesis of Parkinson disease and suggestions for future research. Part I SO JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY LA English DT Review DE Lewy bodies; Parkinson disease; synucleinopathies ID LEWY-BODY-DISEASE; ALPHA-SYNUCLEIN PATHOLOGY; AUTONOMIC NERVOUS-SYSTEM; SLEEP BEHAVIOR DISORDER; SUBSTANTIA-NIGRA; ENVIRONMENTAL-FACTORS; CIGARETTE-SMOKING; BRAIN PATHOLOGY; RISK-FACTORS; DEMENTIA C1 Univ Louisville, Dept Neurol, Sch Med, Movement Disorder Program, Louisville, KY 40202 USA. Prince Wales Med Res Inst, Sydney, NSW, Australia. NINDS, NIH, Baltimore, MD USA. Rush Univ, Med Ctr, Rush Med Coll, Chicago, IL 60612 USA. Mayo Clin, Coll Med, Rochester, MN USA. Hop La Pitie Salpetriere, Salpetriere, France. Univ Bristol, Bristol, Avon, England. Mayo Clin Jacksonville, Jacksonville, FL 32224 USA. Toronto Western Hosp, Toronto, ON M5T 2S8, Canada. Univ Calif Los Angeles, Los Angeles, CA USA. Parkinson Inst, Sunnyvale, CA USA. Univ Tubingen, Tubingen, Germany. NIA, NIH, Bethesda, MD 20892 USA. Kings Coll London, London WC2R 2LS, England. Sackler Sch Med, Rabin Med Ctr, Petah Tiqwa, Israel. Boston Univ, Sch Med, Boston, MA 02215 USA. Univ Virginia, Hlth Syst, Charlottesville, VA USA. Johns Hopkins Sch Med, Baltimore, MD USA. RP Litvan, I (reprint author), Univ Louisville, Dept Neurol, Sch Med, Movement Disorder Program, A Bldg,Room 113,500 S Preston St, Louisville, KY 40202 USA. EM i.litvan@louisville.edu RI Hardy, John/C-2451-2009; Halliday, Glenda/E-8555-2011; OI Halliday, Glenda/0000-0003-0422-8398; Dickson, Dennis W/0000-0001-7189-7917; Litvan, Irene/0000-0002-3485-3445 FU Medical Research Council [G0701075]; NIA NIH HHS [R01 AG024040]; Parkinson's UK [G-0907] NR 80 TC 68 Z9 72 U1 2 U2 7 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0022-3069 J9 J NEUROPATH EXP NEUR JI J. Neuropathol. Exp. Neurol. PD APR PY 2007 VL 66 IS 4 BP 251 EP 257 DI 10.1097/nen.0b013e3180415e42 PG 7 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA 173DD UT WOS:000246852800001 PM 17413315 ER PT J AU Lee, JI Metman, LV Ohara, S Dougherty, PM Kim, JH Lenz, FA AF Lee, J. I. Metman, L. Verhagen Ohara, S. Dougherty, P. M. Kim, J. H. Lenz, F. A. TI Internal pallidal neuronal activity during mild drug-related dyskinesias in Parkinson's disease: Decreased firing rates and altered firing patterns SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID SOMATIC SENSORY NUCLEUS; MPTP-INDUCED PARKINSONISM; SINGLE-UNIT ANALYSIS; GLOBUS-PALLIDUS; SPIKE TRAINS; SUBTHALAMIC NUCLEUS; BASAL GANGLIA; STEREOTAXIC NEUROSURGERY; RECURRENCE PLOTS; FAVORED PATTERNS AB The neuronal basis of hyperkinetic movement disorders has long been unclear. We now test the hypothesis that changes in the firing pattern of neurons in the globus pallidus internus (GPi) are related to dyskinesias induced by low doses of apomorphine in patients with advanced Parkinson's disease (PD). During pallidotomy for advanced PD, the activity of single neurons was studied both before and after administration of apomorphine at doses just adequate to induce dyskinesias (21 neurons, 17 patients). After the apomorphine injection, these spike trains demonstrated an initial fall in firing from baseline. In nine neurons, the onset of ON was simultaneous with that of dyskinesias. In these spike trains, the initial fall in firing rate preceded and was larger than the fall at the onset of ON with dyskinesias. Among the three neurons in which the onset of ON occurred before that of dyskinesias, the firing rate did not change at the time of onset of dyskinesias. After injection of apomorphine, dyskinesias during ON with dyskinesias often fluctuated between transient periods with dyskinesias and those without. Average firing rates were not different between these two types of transient periods. Transient periods with dyskinesias were characterized by interspike interval (ISI) independence, stationary spike trains, and higher variability of ISIs. A small but significant group of neurons demonstrated recurring ISI patterns during transient periods of ON with dyskinesias. These results suggest that mild dyskinesias resulting from low doses of apomorphine are related to both low GPi neuronal firing rates and altered firing patterns. C1 Johns Hopkins Univ Hosp, Dept Neurosurg, Baltimore, MD 21287 USA. Sungkyunkwan Univ, Dept Neurosurg, Samsung Med Ctr, Sch Med, Seoul, South Korea. Rush Univ, Ctr Med, Chicago, IL 60612 USA. Univ Texas, MD Anderson Canc Ctr, Dept Anesthesiol, Houston, TX 77030 USA. Kyoto Kizugawa Hosp, Dept Neurosurg, Kyoto, Japan. NINDS, Expt Therapeut Branch, NIH, Bethesda, MD 20892 USA. RP Lenz, FA (reprint author), Johns Hopkins Univ Hosp, Dept Neurosurg, Meyer 8-181, Baltimore, MD 21287 USA. EM flenz1@jhmi.edu OI Dougherty, Patrick/0000-0002-2177-2734 FU NINDS NIH HHS [NS-39933, NS-40059, R01 NS-38394] NR 72 TC 23 Z9 24 U1 0 U2 3 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD APR PY 2007 VL 97 IS 4 BP 2627 EP 2641 DI 10.1152/jn.00443.2006 PG 15 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA 188OT UT WOS:000247929900007 PM 17215512 ER PT J AU Blivis, D Mentis, GZ O'Donovan, MJ Lev-Tov, A AF Blivis, D. Mentis, G. Z. O'Donovan, M. J. Lev-Tov, A. TI Differential effects of opioids on sacrocaudal afferent pathways and central pattern generators in the neonatal rat spinal cord SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID SUBSTANTIA-GELATINOSA NEURONS; CENTRAL-NERVOUS-SYSTEM; IMMUNOHISTOCHEMICAL LOCALIZATION; DORSAL HORN; P RELEASE; IN-VITRO; HINDLIMB LOCOMOTION; POTASSIUM CURRENTS; TRIGEMINAL NUCLEUS; INHIBIT SUBSTANCE AB The effects of opioids on sacrocaudal afferent (SCA) pathways and the pattern-generating circuitry of the thoracolumbar and sacrocaudal segments of the spinal cord were studied in isolated spinal cord and brain stem-spinal cord preparations of the neonatal rat. The locomotor and tail moving rhythm produced by activation of nociceptive and nonnociceptive sacrocaudal afferents was completely blocked by specific application of the mu-opioid receptor agonist [D-Ala(2), N-Me-Phe(4), Gly(5)-ol]-enkephalin acetate salt (DAMGO) to the sacrocaudal but not the thoracolumbar segments of the spinal cord. The rhythmic activity could be restored after addition of the opioid receptor antagonist naloxone to the experimental chamber. The opioid block of the SCA-induced rhythm is not due to impaired rhythmogenic capacity of the spinal cord because a robust rhythmic activity could be initiated in the thoracolumbar and sacrocaudal segments in the presence of DAMGO, either by stimulation of the ventromedial medulla or by bath application of N-methyl-D-aspartate/serotonin. We suggest that the opioid block of the SCA-induced rhythm involves suppression of synaptic transmission through sacrocaudal interneurons interposed between SCA and the pattern-generating circuitry. The expression of mu opioid receptors in several groups of dorsal, intermediate and ventral horn interneurons in the sacrocaudal segments of the cord, documented in this study, provides an anatomical basis for this suggestion. C1 Hebrew Univ Jerusalem, Sch Med, Dept Anat & Cell Biol, IL-91010 Jerusalem, Israel. Natl Inst Hlth, Sect Dev Neurobiol, Bethesda, MD USA. RP Lev-Tov, A (reprint author), Hebrew Univ Jerusalem, Sch Med, Dept Anat & Cell Biol, IL-91010 Jerusalem, Israel. EM aharonl@ekmd.huji.ac.il RI Blivis, Dvir/F-9385-2012; o'donovan, michael/A-2357-2015 OI Blivis, Dvir/0000-0001-6203-7325; o'donovan, michael/0000-0003-2487-7547 FU Intramural NIH HHS NR 65 TC 21 Z9 21 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD APR PY 2007 VL 97 IS 4 BP 2875 EP 2886 DI 10.1152/jn.01313.2006 PG 12 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA 188OT UT WOS:000247929900028 PM 17287435 ER PT J AU Meunier, S Russmann, H Simonetta-Moreau, M Hallett, M AF Meunier, Sabine Russmann, Heike Simonetta-Moreau, Marion Hallett, Mark TI Changes in spinal excitability after PAS SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID HUMAN MOTOR CORTEX; TRANSCRANIAL MAGNETIC STIMULATION; PAIRED ASSOCIATIVE STIMULATION; PRESYNAPTIC INHIBITION; RECRUITMENT GAIN; DIFFERENT SIZES; H-REFLEX; PLASTICITY; MOTONEURONS; UNITS AB Repetitive pairing of a peripheral stimulation with a magnetic transcortical stimulation (PAS) is widely used to induce plastic changes in the human motor cortex noninvasively. Based on the contrast between PAS-induced increase of corticospinal excitability and absence of PAS-induced increase of the spinal F wave size, it has been generally accepted that PAS-induced plasticity is cortical in origin. Here, instead of F waves, we used H reflex recruitment curves to assess spinal excitability, and we demonstrate that PAS induces parallel changes in cortical and spinal excitability. C1 NINDS, Human Motor Control Sect, NIH, Bethesda, MD 20892 USA. INSERM, U731, Paris, France. Univ Paris 06, Unite Mixte Rech S731, Paris, France. INSERM, U 825, F-31059 Toulouse, France. Hop Purpan, Federat Neurol, Toulouse, France. RP Meunier, S (reprint author), UPMC, INSERM, U731, Serv Readaptat Fonct,Hop Salpetriere, 47 Bd Hop, F-75651 Paris 13, France. EM meunier.sabine@free.fr RI meunier, sabine/G-7622-2014 OI meunier, sabine/0000-0002-6167-4602 FU Intramural NIH HHS NR 27 TC 43 Z9 43 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD APR PY 2007 VL 97 IS 4 BP 3131 EP 3135 DI 10.1152/jn.01086.2006 PG 5 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA 188OT UT WOS:000247929900051 PM 17251364 ER PT J AU Kador, PF Blessing, K Randazzo, J Makita, J Wyman, M AF Kador, Peter F. Blessing, Karen Randazzo, James Makita, Jun Wyman, Milton TI Evaluation of the vascular targeting agent combretastatin a-4 prodrug on retinal Neovascularization in the galactose-fed dog SO JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS LA English DT Article ID HUMAN ENDOTHELIAL-CELLS; A4 PHOSPHATE; DIABETIC-RETINOPATHY; PHASE-I; ADVANCED CANCER; VESSEL CHANGES; ANGIOGENESIS; INHIBITION; TUMORS; CARCINOMA AB Purpose: Combretastatin A-4 (CA-4) is a vascular targeting agent known to rapidly shut off blood flow in new vessels and, as a result, regress neovascularization. In this pilot study, the ability of CA-4 to modify retinal neovascularization, which results in altered retinal vessel blood flow and retinal permeability, was evaluated in aphakic long-term galactose-fed beagles, an animal model that develops diabetes-like retinal neovascularization. Methods: Two (2) groups of aphakic dogs, each group comprised of 4 galactose-fed dogs and 2 age-matched controls dogs, were utilized. Each group initially received the combretastatin A-4-phosphate prodrug (CA-4P) as either sub-Tenon's injections, administered at the corneoscleral junction, or intravitreal injections. Six (6) weeks after this treatment, all dogs also received systemic (intravenous) injections of CA-4P. Retinal vascular changes were monitored at 2-week intervals by fluorescein angiography. Results: All galactose-fed dogs demonstrated the presence of retinal neovascular lesions by fluorescein angiograms. Fluorescein leakage or perfusion through neovascular vessels was not altered by either sub-Tenon's, intravitreal, or systemic CA-4P administration. Whereas CA-4P was well tolerated by the healthy eyes of the control animals, its administration to some galactose-fed dogs was associated with corneal edema and increases in intraocular pressure following sub-Tenon's and intraocular injections. Conclusions: Neovascularization in the galactose-fed dog progresses over a period of years, similar to that observed with clinical diabetic retinopathy. The failure of CA-4P to ameliorate neovascularization suggests that chronic, long-term administration may be required to destroy the slowly growing retinal endothelial cells. C1 NEI, Lab Ocular Therapeut, NIH, Bethesda, MD 20892 USA. Univ Nebraska, Med Ctr, Coll Med, Dept Ophthalmol, Omaha, NE USA. Univ Nebraska, Med Ctr, Coll Pharm, Dept Pharmaceut Sci, Omaha, NE USA. Univ Fukui, Sch Med, Dept Ophthalmol, Fukui 910, Japan. RP Kador, PF (reprint author), NEI, Lab Ocular Therapeut, NIH, Bethesda, MD 20892 USA. EM pkador@unmc.edu NR 42 TC 14 Z9 16 U1 0 U2 0 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1080-7683 J9 J OCUL PHARMACOL TH JI J. Ocular Pharmacol. Ther. PD APR PY 2007 VL 23 IS 2 BP 132 EP 142 DI 10.1089/jop.2006.0103 PG 11 WC Ophthalmology; Pharmacology & Pharmacy SC Ophthalmology; Pharmacology & Pharmacy GA 159BK UT WOS:000245840200006 PM 17444801 ER PT J AU Khan, AA Iadarola, M Yang, HYT Dionne, RA AF Khan, Asma A. Iadarola, Michael Yang, Hslu-Ying T. Dionne, Raymond A. TI Expression of COX-1 and COX-2 in a clinical model of acute inflammation SO JOURNAL OF PAIN LA English DT Article DE COX-1; COX-2; cyclooxygenase; inflammation; lipopolysaccharides; NSAID; prostaglandin; polymorphonuclear ID HUMAN GINGIVAL FIBROBLASTS; CYCLOOXYGENASE-2 MESSENGER-RNA; NITRIC-OXIDE SYNTHASE; IMMUNOREACTIVE BRADYKININ; INDUCIBLE CYCLOOXYGENASE; PROSTAGLANDIN PRODUCTION; TISSUE-LEVELS; IN-VIVO; ISOFORMS; RAT AB Cyclooxygenase (COX) plays an important role in the induction of pain and inflammation as well as the analgesic actions of NSAIDs and coxibs. This study evaluates the expression of the two isoforms COX-1 and COX-2 in a clinical model in which the surgical removal of impacted third molars is used to evaluate the analgesic activity of anti-inflammatory drugs. A 3-mm punch biopsy was performed on the oral mucosa overlying 1 impacted third molar immediately before extraction of 2 impacted lower third molars. After the second tooth was extracted, a second biopsy was performed adjacent to the surgical site either immediately after surgery or 30, 60, or 120 minutes after surgery. RNA was extracted from the biopsy specimens, and RT-PCR was performed to assess mRNA levels of COX-1, COX-2, and glyceraldehyde-3-phosphate dehydrogenase (G3PDH). The RT-PCR products in the biopsy specimens were normalized to G3PDH and compared with baseline. COX-2 mRNA was progressively increased at 30, 60, and 120 minutes after surgery (P < .05); COX-1 mRNA was transiently decreased at 60 minutes during the postsurgical period (P < .05). The results demonstrate peripheral elevation of COX-2 after tissue injury, which may contribute to increased prostaglandin E-2 at the site of injury, pain onset, and the analgesic activity of both nonselective NSAIDs and selective COX-2 inhibitors. Perspective: This clinical study uses a physiologically relevant model to determine the time course of expression of COX-1 and COX-2 in acute inflammation of the human oral mucosa. This study furthers our understanding of the contribution of the COX isoforms to acute pain.(C) 2007 by the American Pain Society. C1 NINR, NIH, Bethesda, MD 20892 USA. Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD USA. RP Dionne, RA (reprint author), NINR, NIH, 10 Ctr Dr,Room 2-NE-1339, Bethesda, MD 20892 USA. EM dionner@mail.nih.gov FU Intramural NIH HHS [Z99 NR999999] NR 37 TC 24 Z9 25 U1 1 U2 10 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND SN 1526-5900 J9 J PAIN JI J. Pain PD APR PY 2007 VL 8 IS 4 BP 349 EP 354 DI 10.1016/j.jpain.2006.10.004 PG 6 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA 161OV UT WOS:000246025800008 PM 17270500 ER PT J AU Ivanova, AV Ivanov, SV Pascal, V Lumsden, JM Ward, JM Morris, N Tessarolo, L Anderson, SK Lerman, MI AF Ivanova, A. V. Ivanov, S. V. Pascal, V. Lumsden, J. M. Ward, J. M. Morris, N. Tessarolo, L. Anderson, S. K. Lerman, M. I. TI Autoimmunity, spontaneous tumourigenesis, and IL-15 insufficiency in mice with a targeted disruption of the tumour suppressor gene FusI SO JOURNAL OF PATHOLOGY LA English DT Article DE Fus1 knockout; autoimmunity; tumour suppressor; IL-15 ID NATURAL-KILLER-CELLS; SYSTEMIC-LUPUS-ERYTHEMATOSUS; HOMOZYGOUS DELETION REGION; NK CELLS; IN-VIVO; DEFICIENT MICE; HUMAN CANCER; EXPRESSION; MATURATION; DIFFERENTIATION AB The Fus1 gene resides in the critical 3p21.3 human chromosomal region deleted in lung and breast cancers. Recently, the tumour suppressor properties of Fus1 were confirmed experimentally by intra-tumoural administration of Fus1 that suppressed experimental lung metastasis in mice. We generated Fusi-deficient mice that were viable, fertile, and demonstrated a complex immunological phenotype. Animals with a disrupted Fus1 gene developed signs of autoimmune disease, such as vasculitis, glomerulonephritis, anaemia, circulating autoantibodies, and showed an increased frequency of spontaneous vascular tumours. Preliminary analysis of immune cell populations revealed a consistent defect in INK cell maturation in Fus1 null mice that correlated with changes in the expression of IL15. Injection of IL-15 into Fus1 knockout mice completely rescued the NK cell maturation defect. Based on these results, we propose the hypothesis that Fus1 deficiency affects NK cell maturation through the reduction of IL-15 production but does not directly alter their developmental capacity. Since acquired immunity was not affected in Fus1-deficient animals, we suggest a relationship between the Fus1 protein and the regulation of innate immunity via IL-15 production. The increased frequency of spontaneous cancers and the development of an autoirmnune syndrome in Fus1 null mice imply that these mice could serve as a model for studying molecular mechanisms of anti-tumour immunity and autoimmunity. Published in 2007 by John Wiley & Sons, Ltd. C1 NCI, Immunol Lab, Ctr Canc Res, Frederick, MD 21702 USA. NCI, Expt Immunol Lab, Ctr Canc Res, Frederick, MD 21702 USA. NCI, Mol Canc Genet Program, Ctr Canc Res, Frederick, MD 21702 USA. NCI, Basic Res Lab, Ctr Canc Res, Frederick, MD 21702 USA. NCI, Lab Anim Sci Program, Frederick, MD 21702 USA. NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA. NIAID, Comparat Med Branch, Bethesda, MD 20892 USA. SoBran Inc, Bethesda, MD 20892 USA. RP Ivanova, AV (reprint author), NYU, Dept Cardiothorac Surg, Med Ctr, 550 1St Ave, New York, NY 10016 USA. EM alla.ivanova@med.nyu.edu RI Anderson, Stephen/B-1727-2012 OI Anderson, Stephen/0000-0002-7856-4266 FU Intramural NIH HHS NR 51 TC 19 Z9 20 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0022-3417 J9 J PATHOL JI J. Pathol. PD APR PY 2007 VL 211 IS 5 BP 591 EP 601 DI 10.1002/path.2146 PG 11 WC Oncology; Pathology SC Oncology; Pathology GA 154BN UT WOS:000245481900012 PM 17318811 ER PT J AU Brown, RT Fuemmeler, B Anderson, D Jamieson, S Simonian, S Hall, RK Brescia, F AF Brown, Ronald T. Fuemmeler, Bernard Anderson, Deborah Jamieson, Sara Simonian, Susan Hall, Rayna Kneuper Brescia, Frank TI Adjustment of children and their mothers with breast cancer SO JOURNAL OF PEDIATRIC PSYCHOLOGY LA English DT Article DE adjustment; breast cancer; social support ID POSTTRAUMATIC-STRESS-DISORDER; PSYCHOLOGICAL DISTRESS; PSYCHOSOCIAL ADJUSTMENT; FAMILY; ILLNESS; SCALE; UNCERTAINTY; DEPRESSION; SURVIVORS; QUALITY AB Objective To examine the adjustment of children of mothers with both active and nonactive breast cancers in comparison with a healthy community control sample. Methods Participants included 80 mothers and their children. Half of the mothers had breast cancer or a history of breast cancer. Children in both groups ranged in age from 8 to 19 years. Assessments included measures of maternal stressors and resources, maternal and child adjustment and posttraumatic stress, and maternal coping and illness uncertainty reported by both mothers and their children. Results Few differences were found between the groups, although there was a trend for girls of mothers with breast cancer to have a higher frequency of depressive symptoms. Children of mothers who perceived support from friends and family had fewer depressive symptoms, after we controlled for child gender.Conclusions The social support perceived by mothers with breast cancer may serve as a protective factor for their children's psychological adjustment. C1 Temple Univ, Dept Publ Hlth, Philadelphia, PA 19140 USA. NCI, Bethesda, MD 20892 USA. Coll Charleston, Charleston, SC 29401 USA. RP Brown, RT (reprint author), Temple Univ, Dept Publ Hlth, 3307 N Broad St,300 Jones Hall, Philadelphia, PA 19140 USA. EM rtbrown@temple.edu NR 40 TC 19 Z9 20 U1 0 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0146-8693 J9 J PEDIATR PSYCHOL JI J. Pediatr. Psychol. PD APR PY 2007 VL 32 IS 3 BP 297 EP 308 DI 10.1093/jpepsy/jsl015 PG 12 WC Psychology, Developmental SC Psychology GA 145BL UT WOS:000244840200007 PM 16837738 ER PT J AU Tanda, G Ebbs, AL Kopajtic, TA Elias, LM Campbell, BL Newman, AH Katz, JL AF Tanda, Gianluigi Ebbs, Aaron L. Kopajtic, Theresa A. Elias, Lyn M. Campbell, Bettye L. Newman, Amy H. Katz, Jonathan L. TI Effects of muscarinic M-1 receptor blockade on cocaine-induced elevations of brain dopamine levels and locomotor behavior in rats SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID VENTRAL TEGMENTAL AREA; IN-VIVO MICRODIALYSIS; NUCLEUS-ACCUMBENS; CHOLINERGIC-RECEPTORS; M2-MUSCARINIC DRUGS; PREFRONTAL CORTEX; UPTAKE INHIBITORS; D-AMPHETAMINE; ANALOGS; RELEASE AB Cholinergic muscarinic systems have been shown to influence dopaminergic function in the central nervous system. In addition, previous studies of benztropine analogs that inhibit dopamine uptake and show antagonism at muscarinic receptors show these drugs to be less effective than cocaine in producing its various prototypic effects such as locomotor stimulation. Because previous pharmacological studies on these topics have used nonselective M-1 antagonists, we examined the interactions of preferential M-1 muscarinic antagonists and cocaine. Dose-dependent increases in extracellular levels of dopamine in selected brain areas, the nucleus accumbens (NAc) shell and core, and the prefrontal cortex, were produced by cocaine but not by the preferential M-1 antagonists telenzepine and trihexyphenidyl. When administered with cocaine, however, both M-1 antagonists dose-dependently increased the effects of cocaine on dopamine in the NAc shell, and these effects were selective in that they were not obtained in the NAc core or in the prefrontal cortex. Telenzepine also increased locomotor activity, although the effect was small compared with that of cocaine. The locomotor stimulant effects of trihexyphenidyl, in contrast, approached those of cocaine. Telenzepine attenuated, whereas trihexyphenidyl enhanced the locomotor stimulant effects of cocaine, with neither drug facilitating cocaine-induced stereotypy. The present results indicate that preferential antagonist effects at muscarinic M 1 receptors do not uniformly alter all of the effects of cocaine, nor do they explain the differences in effects of cocaine and benztropine analogs, and that the alterations in dopamine levels in the NAc shell do not predict the behavioral effects of the interactions with cocaine. C1 NIDA, Res Branch, Dept Hlth & Human Serv, NIH, Baltimore, MD USA. RP Tanda, G (reprint author), NIDA, Dept Hlth & Human Serv, NIH, Intramural Res Program,Med Discovery Res Branch, 5500 nathan Shock Dr, Baltimore, MD 21224 USA. EM gtanda@intra.nida.nih.gov RI Tanda, Gianluigi/B-3318-2009; Haselton, Lyn/N-1260-2016 OI Tanda, Gianluigi/0000-0001-9526-9878; FU Intramural NIH HHS NR 40 TC 31 Z9 31 U1 3 U2 5 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD APR PY 2007 VL 321 IS 1 BP 334 EP 344 DI 10.1124/jpet.106.118067 PG 11 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 147FU UT WOS:000244989600037 PM 17255465 ER PT J AU Utsuki, T Uchimura, N Irikura, M Moriuchi, H Holloway, HW Yu, QS Spangler, EL Mamczarz, J Ingram, DK Irie, T Greig, NH AF Utsuki, Tadanobu Uchimura, Nao Irikura, Mitsuru Moriuchi, Hiroshi Holloway, Harold W. Yu, Qian-Sheng Spangler, Edward L. Mamczarz, Jacek Ingram, Donald K. Irie, Tetsumi Greig, Nigel H. TI Preclinical investigation of the topical administration of phenserine: Transdermal flux, cholinesterase inhibition, and cognitive efficacy SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID 14-UNIT T-MAZE; ALZHEIMERS-DISEASE; PERCUTANEOUS-ABSORPTION; MEMORY IMPAIRMENT; STRATUM-CORNEUM; RATS; ACETYLCHOLINESTERASE; PERMEABILITY; MODEL; SKIN AB Phenserine (PS) was designed as a selective acetylcholinesterase (AChE) inhibitor, with a tartrate form (PST) for oral administration in mild to moderate Alzheimer's disease (AD). Recent phase 3 trials of PST in Europe indicate that any clinically relevant activity of PST may be limited by its duration of action. Like many oral drugs, bioavailability and plasma concentrations of PST are regulated by hepatic and gastrointestinal first-pass effects. To minimize the kinetic limitations of first-pass metabolism, transdermal formulations of PS and PST (ointment/ patch) were developed and characterized in vitro and in vivo. Initial in vitro kinetic characterization of PS or PST formulations used a diffusion cell chamber and skin samples isolated from hairless mice. Liquid paraffin and fatty alcohol/propylene glycol FAPG) were found to be suitable vehicles for ointment formulation. Addition of a penetration enhancer, 1-[2-(decylthio)ethyl]azacyclopentane-2-one (HPE-101), improved stratum corneum permeability. Application of the optimal formulation of PS/HPE101/FAPG to the shaved back of rats resulted in significantly lowered plasma and brain AChE activities and improved cognitive performance in animals with scopolamine-induced cognitive impairment. These results suggest that the transdermal application of AChE inhibitors may represent an effective therapeutic strategy for AD. Particular benefits over oral therapies might include avoiding first-pass metabolic effects and improved dosing compliance. C1 NIA, Drug Design Dev Sect, Neurosci Lab, NIH, Baltimore, MD 21224 USA. NIA, Lab Expt Gerontol, NIH, Baltimore, MD 21224 USA. Louisiana State Univ, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. Kumamoto Univ, Grad Sch Med & Pharmaceut Sci, Div Clin Chem & Informat, Kumamoto, Japan. RP Greig, NH (reprint author), 5600 Nathan Shock Dr, Baltimore, MD 21224 USA. EM greign@grc.nia.nih.gov FU Intramural NIH HHS NR 39 TC 9 Z9 9 U1 0 U2 4 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD APR PY 2007 VL 321 IS 1 BP 353 EP 361 DI 10.1124/jpet.106.118000 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 147FU UT WOS:000244989600039 PM 17255466 ER PT J AU Solinas, M Tanda, G Justinova, Z Wertheim, CE Yasar, S Piomelli, D Vadivel, SK Makriyannis, A Goldberg, SR AF Solinas, Marcello Tanda, Gianluigi Justinova, Zuzana Wertheim, Carrie E. Yasar, Sevil Piomelli, Daniele Vadivel, Subramanian K. Makriyannis, Alexandros Goldberg, Steven R. TI The endogenous cannabinoid anandamide produces delta-9-tetrahydrocannabinol-like discriminative and neurochemical effects that are enhanced by inhibition of fatty acid amide hydrolase but not by inhibition of anandamide transport SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID VANILLOID RECEPTORS; SQUIRREL-MONKEYS; RATS; (R)-METHANANDAMIDE; DELTA(9)-THC; HYDROLYSIS; AM404; RECOGNITION; SELECTIVITY; MODULATION AB Anandamide is an endogenous ligand for brain cannabinoid CB(1) receptors, but its behavioral effects are difficult to measure due to rapid inactivation. Here we used a drug-discrimination procedure to test the hypothesis that anandamide, given i.v. or i.p., would produce discriminative effects like those of delta-9-tetrahydrocannabinol (THC) in rats when its metabolic inactivation was inhibited. We also used an in vivo microdialysis procedure to investigate the effects of anandamide, given i.v. or i.p., on dopamine levels in the nucleus accumbens shell in rats. When injected i.v., methanandamide (AM-356), a metabolically stable anandamide analog, produced clear dose-related THC-like discriminative effects, but anandamide produced THC-like discriminative effects only at a high 10-mg/kg dose that almost eliminated lever-press responding. Cyclohexyl carbamic acid 3'-carbamoyl- biphenyl-3-yl ester (URB-597), an inhibitor of fatty acid amide hydrolase (FAAH), the main enzyme responsible for metabolic inactivation of anandamide, produced no THC-like discriminative effects alone but dramatically potentiated discriminative effects of anandamide, with 3 mg/kg anandamide completely substituting for the THC training dose. URB-597 also potentiated the ability of anandamide to increase dopamine levels in the accumbens shell. The THC-like discriminative-stimulus effects of anandamide after URB-597 and methanandamide were blocked by the CB(1) receptor antagonist rimonabant, but not the vanilloid VR(1) receptor antagonist capsazepine. Surprisingly, the anandamide transport inhibitors N-(4-hydroxyphenyl) eicosa-5,8,11,14-tetraenamide (AM-404) and N-(3furylmethyl)eicosa-5,8,11,14-tetraenamide (UCM-707) did not potentiate THC-like discriminative effects of anandamide or its dopamine-elevating effects. Thus, anandamide has THC-like discriminative and neurochemical effects that are enhanced after treatment with a FAAH inhibitor but not after treatment with transport inhibitors, suggesting brain area specificity for FAAH versus transport/FAAH inactivation of anandamide. C1 NIDA, Preclin Pharmacol Sect, Behav Neurosci Res Branch, NIH,DHHS, Baltimore, MD 21224 USA. NIDA, Psychobiol Sect, Med Discovery Res Branch, NIH,DHHS, Baltimore, MD 21224 USA. Univ Poitiers, CNRS 6187, Lab Biol & Physiol Cellulaires, Poitiers, France. Univ Maryland, Sch Med, Maryland Psychiat Res Ctr, Baltimore, MD 21201 USA. Johns Hopkins Univ, Sch Med, Div Geriatr Med & Gerontol, Baltimore, MD USA. Univ Calif Irvine, Dept Pharmacol, Irvine, CA 92717 USA. Northeastern Univ, Bouve Coll Hlth Sci, Ctr Drug Discovery, Boston, MA 02115 USA. RP Goldberg, SR (reprint author), NIDA, Preclin Pharmacol Sect, Intramural Res Program, NIH,DHHS, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM sgoldber@intra.nida.nih.gov RI Tanda, Gianluigi/B-3318-2009; Justinova, Zuzana/A-9109-2011; Solinas, Marcello/M-3500-2016 OI Tanda, Gianluigi/0000-0001-9526-9878; Justinova, Zuzana/0000-0001-5793-7484; Solinas, Marcello/0000-0002-0664-5964 FU Intramural NIH HHS; NIDA NIH HHS [DA09158, DA12413, DA12447, DA7215] NR 42 TC 71 Z9 73 U1 2 U2 5 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD APR PY 2007 VL 321 IS 1 BP 370 EP 380 DI 10.1124/jpet.106.114124 PG 11 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 147FU UT WOS:000244989600041 PM 17210800 ER PT J AU Plested, AJ Ashby, MC Scimemi, A AF Plested, Andrew J. Ashby, Michael C. Scimemi, Annalisa TI Getting hot under the calyx SO JOURNAL OF PHYSIOLOGY-LONDON LA English DT Editorial Material ID AMPA RECEPTOR DESENSITIZATION; SYNAPSES C1 NICHHD, NIH, Bethesda, MD 20892 USA. NINDS, NIH, Bethesda, MD 20892 USA. RP Plested, AJ (reprint author), NICHHD, NIH, 35 Convent Dr, Bethesda, MD 20892 USA. EM plesteda@mail.nih.gov RI Scimemi, Annalisa/O-5396-2014; OI Scimemi, Annalisa/0000-0003-4975-093X; Plested, Andrew/0000-0001-6062-0832 NR 5 TC 1 Z9 1 U1 0 U2 0 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-3751 J9 J PHYSIOL-LONDON JI J. Physiol.-London PD APR 1 PY 2007 VL 580 IS 1 BP 13 EP 14 DI 10.1113/physiol.2007.129502 PG 2 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA 156KM UT WOS:000245646800006 PM 17303634 ER PT J AU Xu, HL AF Xu, Han-Lun TI On estimating a survival function under the generalized proportional hazards model SO JOURNAL OF STATISTICAL PLANNING AND INFERENCE LA English DT Article DE generalized proportional hazards model; maximum likelihood estimator; invariance property ID MAXIMUM-LIKELIHOOD-ESTIMATION; NONPARAMETRIC-ESTIMATION; RANDOM CENSORSHIP AB Let X and Y be two competing lifetimes with continuous survival functions (F) over bar (t) and (G) over bar (t), respectively, and let beta be a nonnegative random variable with B(b) = P[beta <= b]. A generalized proportional hazards model proposed by Pefia and Rohatgi [1989. Survival function estimation for a generalized proportional hazards model of random censorship. J. Statist. Plann. Inference 22, 371-389] assumed that X and (Y, beta) are independent and (G) over bar (t) = E-B[(F) over bar (t)](beta). When B(b) is uniquely determined by an unknown parameter theta with a mild condition, they used n independent and identically distributed (iid) observations (Z(i), delta(i))(i=1)(n) with Z(i) = min (X-i, Y-i) and delta(i) = 1 (X-i <= Y-i) to find the maximum likelihood estimators (MLEs) of (F) over bar (t) and theta and their large sample properties. In this paper we point out that the estimators of F(t) and theta proposed by Pefia and Rohatgi (1989) are not MLEs in general. Specifically, we prove that Pefia and Rohatgi's estimators (PREs) of (F) over bar (t) and theta are MLEs if and only if the random variable beta is degenerate at a constant b > 0 which was studied by Cheng and Lin [1987. Maximum likelihood estimation of survival function under the Koziol-Green proportional hazards model. Statist. Probab. Lett. 5, 75-80]. It is also shown that Z and delta are always positively correlated under the generalized proportional hazards model. A modification of the generalized proportional hazards model which can be used to fit a data set showing the negative correlation between Z and delta is proposed. A conditionally proportional odds model which assumes P has a negative binomial distribution with size two and parameter theta is introduced and used to conduct simulation studies when sample size is small or moderate. In terms of variance of simulation studies, the MLE is better than the product-limit estimator and PRE. (c) 2006 Elsevier B.V. All rights reserved. C1 NCI, Biometry Res Grp, Bethesda, MD 20892 USA. RP Xu, HL (reprint author), NCI, Biometry Res Grp, EPN-3131,6130 Execut Blvd,MSC 7354, Bethesda, MD 20892 USA. EM jx2b@nih.gov NR 16 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3758 J9 J STAT PLAN INFER JI J. Stat. Plan. Infer. PD APR 1 PY 2007 VL 137 IS 4 BP 1161 EP 1172 DI 10.1016/j.jspi.2006.04 PG 12 WC Statistics & Probability SC Mathematics GA 132PW UT WOS:000243955700006 ER PT J AU Taxman, FS Young, DW Fletcher, BW AF Taxman, Faye S. Young, Douglas W. Fletcher, Bennett W. TI The National Criminal Justice Treatment Practices survey: An overview of the special issue SO JOURNAL OF SUBSTANCE ABUSE TREATMENT LA English DT Editorial Material C1 Virginia Commonwealth Univ, Wilder Sch Govt & Publ Affairs, Richmond, VA 23220 USA. Univ Maryland, Inst Govt Serv & Res, College Pk, MD 20740 USA. NIDA, Bethesda, MD 20892 USA. RP Taxman, FS (reprint author), Virginia Commonwealth Univ, Wilder Sch Govt & Publ Affairs, Richmond, VA 23220 USA. EM fstaxman@vcu.edu FU NIDA NIH HHS [U01 DA016213, U01 DA016213-05] NR 2 TC 5 Z9 5 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0740-5472 J9 J SUBST ABUSE TREAT JI J. Subst. Abus. Treat. PD APR PY 2007 VL 32 IS 3 BP 221 EP 223 DI 10.1016/j.jsat.2006.12.017 PG 3 WC Psychology, Clinical; Substance Abuse SC Psychology; Substance Abuse GA 155ID UT WOS:000245570200001 PM 17383547 ER PT J AU Yende, S Angus, DC Ali, IS Somes, G Newman, AB Bauer, D Garcia, M Harris, TB Kritchevsky, SB AF Yende, Sachin Angus, Derek C. Ali, Ibrabim Sultan Somes, Grant Newman, Anne B. Bauer, Douglas Garcia, Melissa Harris, Tamara B. Kritchevsky, Stephen B. CA Hlth ABC Study TI Influence of comorbid conditions on long-term mortality after pneumonia in older people SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article DE pneumonia; community-acquired pneumonia; CAP; comorbidity; hospitalization ID COMMUNITY-ACQUIRED PNEUMONIA; INFLAMMATORY MARKERS; HIP FRACTURE; RISK-FACTORS; MANS FRIEND; HOSPITALIZATION; PROGNOSIS; POPULATION; SURVIVAL; STROKE AB OBJECTIVES: To test the hypothesis that increased long-term mortality after hospitalization for community-acquired pneumonia (CAP) is independent of comorbid conditions. DESIGN: Prospective observational cohort study in metropolitan areas. SETTING: Memphis, Tennessee, and Pittsburgh, Pennsylvania. PARTICIPANTS: Three thousand seventy-five subjects aged 70 to 79 over 5.2 years. MEASUREMENTS: Unadjusted and adjusted mortality from an initial hospitalization for CAP were compared with mortality from different causes of hospitalization, including cancer, fracture, congestive heart failure (CHF), cerebrovascular accident (CVA), and other causes. Demographics, smoking, nutritional markers, functional status, inflammatory markers, and chronic health conditions were adjusted for. RESULTS: Of the 106 subjects hospitalized for CAP, 22 (20.8%) and 38 (35.8%) died at 1. and 5 years. Subjects hospitalized with CAP had higher mortality than nonhospitalized subjects (adjusted odds ratio (OR) = 7.8, 95% confidence interval (CI) = 4.2-14.4). One- and 5-year mortality after CAP hospitalization were higher than mortality from other causes requiring hospitalization and remained unchanged in multivariable analysis (adjusted OR = 3.5, 95 % CI = 1.5-8.1; adjusted OR = 5.6, 95 % CI = 2.8-11.2, respectively). One- and 5-year mortality after hospitalization for CAP were similar to or higher than mortality after an initial hospitalization for CHF, CVA, or fracture. Rehospitalization was common in subjects hospitalized for CAP and may explain greater long-term mortality. CONCLUSION: In this high-functioning cohort of older persons, an initial hospitalization for CAP was associated with greater long-term mortality, independent of prehospitalization comorbid conditions. Hospitalization for CAP has as serious a prognosis as hospitalization for CHF, stroke, or major fracture. C1 Univ Pittsburgh, Dept Crit Care Med, Clin Res Invest & Syst Modeling Acute Illness Lab, Pittsburgh, PA 15261 USA. Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15261 USA. Univ Tennessee, Div Pulm & Crit Care, Memphis, TN USA. Univ Tennessee, Dept Prevent Med, Memphis, TN USA. Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA. Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA. NIA, Lab Epidemiol Demog & Biometry, Bethesda, MD 20892 USA. Wake Forest Univ, Sch Med, Sticht Ctr Aging, Winston Salem, NC USA. RP Yende, S (reprint author), Univ Pittsburgh, Dept Crit Care Med, Clin Res Invest & Syst Modeling Acute Illness Lab, 641 Scaife Hall,3550 Terrace St, Pittsburgh, PA 15261 USA. EM yendes@upmc.edu RI Angus, Derek/E-9671-2012; Newman, Anne/C-6408-2013; OI Newman, Anne/0000-0002-0106-1150; Kritchevsky, Stephen/0000-0003-3336-6781 FU Intramural NIH HHS; NHLBI NIH HHS [R01HL74104]; NIA NIH HHS [N01-AG-6-2101, N01-AG-6-2103, N01-AG-6-2106]; NIAID NIH HHS [AI27913, AI39482]; NIGMS NIH HHS [R01 GM061992]; PHS HHS [R01 G61992] NR 23 TC 71 Z9 74 U1 0 U2 4 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 BP 518 EP 525 DI 10.1111/j.1532-5415.2007.01100.x PG 8 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 150QK UT WOS:000245231100005 PM 17397429 ER PT J AU Faulkner, KA Redfern, MS Cauley, JA Landsittel, DF Studenski, SA Rosano, C Simonsick, EM Harris, TB Shorr, RI Ayonayon, HN Newman, AB AF Faulkner, Kimberly A. Redfern, Mark S. Cauley, Jane A. Landsittel, Douglas F. Studenski, Stephaine A. Rosano, Caterina Simonsick, Eleanor M. Harris, Tamara B. Shorr, Ronald I. Ayonayon, Hilsa N. Newman, Anne B. CA Hlth, Aging, Body Composition Stud TI Multitasking: Association between poorer performance and a history of recurrent falls SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article; Proceedings Paper CT 58th Annual Meeting of the Gerontological-Society-of-America CY NOV 21, 2005 CL Orlando, FL SP Gerontol Soc Amer DE attention; multitasking; dual task; visual-spatial; reaction time; cognition ID DUAL-TASK; OLDER-ADULTS; ATTENTIONAL DEMANDS; COGNITIVE TASKS; ELDERLY-PEOPLE; WALKING; GAIT; POSTURE; BALANCE; TALKING AB OBJECTIVES: To examine the association between poorer performance on concurrent walking and reaction time and recurrent falls. DESIGN: Cross-sectional analysis. SETTING: Community. PARTICIPANTS: Three hundred seventy-seven older community-dwelling adults (mean age +/- standard deviation 78 3). MEASUREMENTS: Reaction times on push-button and visual-spatial decision tasks were assessed while seated and while walking a 20-m course (straight walk) and a 20-m course with a turn at 10 in (turn walk). Walking times were recorded while walking only and while performing a reaction-time response. Dual-task performance was calculated as the percentage change in task times when done in dual-task versus single-task conditions. A, history of recurrent falls (>= 2 vs <= 1 falls) in the prior 12 months was self-reported. Multivariate logistic regression models were used to predict the standardized odds ratios (ORs) of recurrent falls history. The standardized unit for dual-task performance ORs was interquartile range/2. RESULTS: On the push-button task during the turn walk, poorer reaction time response (slower) was associated with 28% lower (P = .04) odds of recurrent fall history. On the visual-spatial task, poorer walking-time response (slower) was associated with 34% (P = .02) and 42% (P = .01) higher odds of recurrent falls history on the straight and turn walks, respectively. CONCLUSION: These findings suggest that walking more slowly in response to a visual-spatial decision task may identify individuals at risk for multiple falls. Prospective studies are needed to confirm the prognostic value of poor walking responses in a dual-task setting for multiple falls. C1 Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15261 USA. Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA 15261 USA. Univ Pittsburgh, Dept Geriatr Med, Pittsburgh, PA 15261 USA. Duquesne Univ, Dept Math & Comp Sci, Pittsburgh, PA 15219 USA. NIA, Dept Clin Res, Baltimore, MD 21224 USA. NIA, Dept Epidemiol Demog & Biometry, Bethesda, MD 20892 USA. Univ Tennessee, Memphis, TN USA. Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA. RP Faulkner, KA (reprint author), 130 DeSoto St,Parran 509, Pittsburgh, PA 15261 USA. EM kaf24@pitt.edu RI Newman, Anne/C-6408-2013; Cauley, Jane/N-4836-2015; OI Newman, Anne/0000-0002-0106-1150; Cauley, Jane/0000-0003-0752-4408; Rosano, Caterina/0000-0002-0909-1506; Rosano, Caterina/0000-0002-4271-6010 FU Intramural NIH HHS; NIA NIH HHS [N01-AG-6-2101, N01-AG-6-2103, N01-AG-6-2106, P30 AG024827, T32-AG00181] NR 34 TC 69 Z9 71 U1 1 U2 18 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 BP 570 EP 576 DI 10.1111/j.1532-5415.2007.01147.x PG 7 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 150QK UT WOS:000245231100012 PM 17397436 ER PT J AU Beveridge, CA Ershler, WB Orloff, GJ Sheridan, MJ Shelton, MV AF Beveridge, C. A. Ershler, W. B. Orloff, G. J. Sheridan, M. J. Shelton, M. V. TI Outcomes associated with advancing age in patients with multiple myeloma (MM) receiving stem cell transplant. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Fairfax No Virginia Hematol Oncol, Fairfax, VA USA. Natl Inst Aging, Clin Res Branch, Baltimore, MD USA. Inst Adv Studies Aging, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S55 EP S56 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500161 ER PT J AU Brenes, GA Newman, A Simonsick, E Houston, D Penninx, B Yaffe, K Harris, T Ayonayon, H Rubin, S Satterfield, S Visser, M Kritchevsky, S AF Brenes, G. A. Newman, A. Simonsick, E. Houston, D. Penninx, B. Yaffe, K. Harris, T. Ayonayon, H. Rubin, S. Satterfield, S. Visser, M. Kritchevsky, S. TI Emotional, cognitive and physical reserve and incident functional limitations: Results from the Health ABC Study. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Wake Forest Univ, Sch Med, Winston Salem, NC 20892 USA. Univ Pittsburgh, Baltimore, MD USA. NIA, Bethesda, MD 94143 USA. Vrije Univ Amsterdam, Amsterdam, Netherlands. Univ Calif San Francisco, San Francisco, CA USA. Univ Tennessee, Memphis, TN USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S28 EP S29 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500081 ER PT J AU Fraser, DA Masaki, K Chen, R Ross, GW Petrovitch, H Launer, L Blanchette, PL While, L AF Fraser, D. A. Masaki, K. Chen, R. Ross, G. W. Petrovitch, H. Launer, L. Blanchette, P. L. While, L. TI Family history of dementia and down's syndrome as risk factors for 6-year cognitive decline in elderly Japanese-American men. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Univ Hawaii, Honolulu, HI 96822 USA. Pacific Hlth Res Inst, Honolulu, HI USA. Dept Vet Affairs, Honolulu, HI USA. NIA, Lab Epidemiol Demography & Biometry, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S31 EP S32 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500089 ER PT J AU Giannelli, SV Patel, KV Windham, G Pizzarelli, F Ferrucci, L Guralnik, JM AF Giannelli, S. V. Patel, K. V. Windham, G. Pizzarelli, F. Ferrucci, L. Guralnik, J. M. TI Underascertainment of impaired kidney function among older adults with normal serum creatinine. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 NIH, NIA, Lab Epidemiol Demography & Biometry, Bethesda, MD 20892 USA. Univ Hosp Geneva, Dept Rehabil & Geriatr, Geneva, Switzerland. NIH, NIA, Clin Res Branch, Longitudinal Studies Sect, Baltimore, MD USA. RI Giannelli, Sandra/E-8637-2011 NR 0 TC 1 Z9 1 U1 0 U2 0 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S32 EP S33 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500092 ER PT J AU Hicks, G Shardell, M Miller, R Bandinelli, S Guralnik, J Cherubini, A Lauretani, F Ferrucci, L AF Hicks, G. Shardell, M. Miller, R. Bandinelli, S. Guralnik, J. Cherubini, A. Lauretani, F. Ferrucci, L. TI Low 25(OH)D concentrations are associated with significant pain in older women, but not men: Findings from the InCHIANTI Study. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Univ Delaware, Newark, DE USA. Univ Maryland, Baltimore, MD 21201 USA. INRCA, Florence, Italy. Univ Perugia, I-06100 Perugia, Italy. NIA, Baltimore, MD 21224 USA. Tuscany Reg Hlth Agcy, Florence, Italy. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S81 EP S82 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500240 ER PT J AU Houston, DK Ding, J Tooze, J Lee, J Garcia, M Kanaya, A Tylavsky, FA Newman, AB Visser, M Kritchevsky, SB AF Houston, D. K. Ding, J. Tooze, J. Lee, J. Garcia, M. Kanaya, A. Tylavsky, F. A. Newman, A. B. Visser, M. Kritchevsky, S. B. TI Dietary fats and incident coronary heart disease in older adults: the health ABC study. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Wake Forest Univ, Sch Med, Winston Salem, NC USA. Univ Georgia, Athens, GA 30602 USA. NIA, Bethesda, MD 20892 USA. Univ Calif San Francisco, San Francisco, CA 94143 USA. Univ Tennessee, Memphis, TN USA. Univ Pittsburgh, Pittsburgh, PA USA. Vrije Univ Amsterdam, Amsterdam, Netherlands. NR 0 TC 0 Z9 0 U1 0 U2 2 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S177 EP S178 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500522 ER PT J AU Inzitari, M Newman, AB Yaffe, K Boudreau, R de Rekeneire, N Shorr, R Harris, TB Rosano, C AF Inzitari, M. Newman, A. B. Yaffe, K. Boudreau, R. de Rekeneire, N. Shorr, R. Harris, T. B. Rosano, C. TI Gait speed predicts decline in attention and psychomotor speed in older adults: the health aging and body composition study. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Univ Pittsburgh, Dept Med, Pittsburgh, PA USA. Univ Florence, Unit Geriatr, Florence, Italy. Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15261 USA. Univ Calif San Francisco, Dept Psychiat, San Francisco, CA 94143 USA. Ctr Dis Control, Atlanta, GA 30333 USA. Univ Tennessee, Ctr Hlth Sci, Dept Prevent Med, Memphis, TN 38163 USA. NIA, Lab Epidemiol Demography & Biometry, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S82 EP S83 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500243 ER PT J AU Kritchevsky, SB Lenchik, L Leng, X Ding, J Guralnik, J Ferrucci, L Pahor, M Earnest, C Hsu, F Goodpaster, G AF Kritchevsky, S. B. Lenchik, L. Leng, X. Ding, J. Guralnik, J. Ferrucci, L. Pahor, M. Earnest, C. Hsu, F. Goodpaster, G. TI Body composition and physical function in the lifestyle interventions and independence for elders pilot (LIFE-P) study. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Wake Forest Univ, Sch Med, Sticht Ctr Aging, Winston Salem, NC 27109 USA. Nat Inst Aging, Bethesda, MD USA. Univ Florida, Gainesville, FL USA. Cooper Inst, Dallas, TX USA. Univ Pittsburgh, Pittsburgh, PA USA. RI Earnest, Conrad/A-7860-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S67 EP S67 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500195 ER PT J AU Pang, JH Masaki, K Chen, R White, L Abbott, R Petrovitch, H Ross, GW Ardo, E Blanchette, PL Launer, L AF Pang, J. H. Masaki, K. Chen, R. White, L. Abbott, R. Petrovitch, H. Ross, G. W. Ardo, E. Blanchette, P. L. Launer, L. TI Fish intake is not a predictor of cognitive decline over 6 years: The Honolulu-Asia aging study. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Univ Hawaii, Honolulu, HI 96822 USA. Pacific Hlth res Inst, Honolulu, HI USA. Dept Vet Affairs, Honolulu, HI USA. NIA, Lab Epidemiol Demography & Biomet, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S178 EP S179 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500525 ER PT J AU Perera, S Studenski, SA Newman, AB Simonsick, EM Harris, T Perry, S Schwartz, A Visser, M AF Perera, S. Studenski, S. A. Newman, A. B. Simonsick, E. M. Harris, T. Perry, S. Schwartz, A. Visser, M. TI Subclinical decline in performance among older adults (Health ABC study). SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Univ Pittsburgh, Pittsburgh, PA 15260 USA. NIA, Baltimore, MD 21224 USA. Univ Tennessee, Memphis, TN USA. Univ Calif San Francisco, San Francisco, CA 94143 USA. Vrije Univ Amsterdam, Amsterdam, Netherlands. RI Perera, Subashan/D-7603-2014 NR 0 TC 0 Z9 0 U1 0 U2 2 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S194 EP S194 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500571 ER PT J AU Perera, S Studenski, SA Newman, AB Simonsick, EM Harris, T Perry, S Schwartz, A Visser, M AF Perera, S. Studenski, S. A. Newman, A. B. Simonsick, E. M. Harris, T. Perry, S. Schwartz, A. Visser, M. TI Magnitude of meaningful change in mobility performance tests: Effects of initial performance (Health ABC Study). SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 Univ Pittsburgh, Pittsburgh, PA USA. NIA, Baltimore, MD 21224 USA. Univ Calif San Francisco, San Francisco, CA 94143 USA. Vrije Univ Amsterdam, Amsterdam, Netherlands. RI Perera, Subashan/D-7603-2014 NR 0 TC 1 Z9 1 U1 0 U2 2 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S17 EP S17 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500046 ER PT J AU Shanahan, B Bartels, SJ Bruce, ML Halpain, M Lebowitz, BD Light, E Reynolds, CE Smith, G Streim, JE Unutzer, J AF Shanahan, B. Bartels, S. J. Bruce, M. L. Halpain, M. Lebowitz, B. D. Light, E. Reynolds, C. E. Smith, G. Streim, J. E. Unutzer, J. TI Effectiveness of an online initiative to reverse the impending shortage of geriatric mental health researchers. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Scientific Meeting of the American-Geriatrics-Society CY MAY 02-06, 2007 CL Seattle, WA SP Amer Geriat Soc C1 MediSpin Inc, New York, NY USA. New Hampshire Dartmouth Psychiat Res Ctr, Lebanon, NH USA. Cornell Univ, Weill Med Coll, Dept Psychiat, White Plains, NY USA. Univ Calif San Diego, Div Geriatr Psychiat, San Diego, CA 92103 USA. Univ Calif San Diego, Sam & Rose stein Inst Res Aging, San Diego, CA 92103 USA. NIMH, Bethesda, MD 20892 USA. Univ Pittsburgh, Sch Med, Pittsburgh, PA 15260 USA. Univ Toronto, Dept Psychiat, Toronto, ON, Canada. Univ Penn, Sch Med, Sect Geriatr Psychiat, Philadelphia, PA 19104 USA. Univ Washington, Sch Med, Dept Psychiat & Behav Sci, Seattle, WA 98195 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2007 VL 55 IS 4 SU S BP S135 EP S135 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 157WQ UT WOS:000245752500397 ER PT J AU Campbell, JM Vestal, ML Blank, PS Stein, SE Epstein, JA Yergey, AL AF Campbell, Jennifer M. Vestal, Marvin L. Blank, Paul S. Stein, Stephen E. Epstein, Jonathan A. Yergey, Alfred L. TI Fragmentation of leucine enkephalin as a function of laser fluence in a MALDI TOF-TOF SO JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY LA English DT Article ID COLLISION-INDUCED DISSOCIATION; SURFACE-INDUCED DISSOCIATION; DESORPTION/IONIZATION MASS-SPECTROMETRY; MOLECULAR-DYNAMICS MODEL; DESORPTION IONIZATION; PROTONATED PEPTIDES; INTERNAL ENERGY; ORGANIC-SOLIDS; ION FORMATION; UV-MALDI AB The effects of laser fluence on ion formation in MALDI were studied using a tandem TOF mass spectrometer with a Nd-YAG laser and alpha-cyano hydrocinnamic acid matrix. Leucine enkephalin ionization and fragmentation were followed as a function of laser fluence ranging from the threshold of ion formation to the maximum available, that is, about 280-930 mJ/mm(2). The most notable finding was the appearance of immonium ions at fluence values close to threshold, increasing rapidly and then tapering in intensity with the appearance of typical backbone fragment ions. The data suggest the presence of two distinct environments for ion formation. One is associated with molecular desorption at low values of laser fluence that leads to extensive immonium ion formation. The second becomes dominant at higher fluences, is associated initially with backbone type fragments, but, at the highest values of fluence, progresses to immonium fragments. This second environment is suggestive of ion desorption from large pieces of material ablated from the surface. Arrhenius rate law considerations were used to estimate temperatures associated with the onset of these two processes. C1 NICHHD, Cellular & Mol Biol Lab, NIH, Bethesda, MD 20892 USA. Appl Biosyst Inc, Framingham, MA USA. Natl Inst Stand & Technol, Phys & Chem Properties Div, Gaithersburg, MD 20899 USA. NICHHD, Unit Biol Computat, NIH, Bethesda, MD 20892 USA. RP Yergey, AL (reprint author), NICHHD, Cellular & Mol Biol Lab, NIH, Bldg 10,Room 9D52, Bethesda, MD 20892 USA. EM aly@helix.nih.gov FU Intramural NIH HHS; NICHD NIH HHS [Z01 HD001417-12] NR 55 TC 16 Z9 16 U1 0 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1044-0305 J9 J AM SOC MASS SPECTR JI J. Am. Soc. Mass Spectrom. PD APR PY 2007 VL 18 IS 4 BP 607 EP 616 DI 10.1016/j.jasms.2006.11.008 PG 10 WC Chemistry, Analytical; Chemistry, Physical; Spectroscopy SC Chemistry; Spectroscopy GA 154XH UT WOS:000245540800003 PM 17204430 ER PT J AU Jurewicz, M McDermott, DH Sechler, JM Tinckam, K Takakura, A Carpenter, CB Milford, E Abdi, R AF Jurewicz, Mollie McDermott, David H. Sechler, Joan M. Tinckam, Kathryn Takakura, Ayumi Carpenter, Charles B. Milford, Edgar Abdi, Reza TI Human T and natural killer cells possess a functional renin-angiotensin system: Further mechanisms of angiotensin II-induced inflammation SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Article ID IFN-GAMMA PRODUCTION; IN-VITRO; DENDRITIC CELLS; CONVERTING-ENZYME; MONONUCLEAR-CELLS; KIDNEY-DISEASE; RENAL INJURY; AT2 RECEPTOR; EXPRESSION; MACROPHAGES AB The renin-angiotensin system (RAS) plays an important role in the regulation of inflammation and in the progression of chronic kidney disease. Accumulation of inflammatory cells into the renal parenchyma has been a hallmark of chronic kidney disease; however, little is known concerning the presence and the function of RAS elements in T and natural killer (NK) cells. Here is reported a co-stimulatory effect of angiotensin II (AngII) by showing an augmentation of mitogen and anti-CD3-stimulated T and NK cell proliferation with AngII treatment. Angiotensinogen and AngI also generated the same effect, suggesting that NK and T cells have functional renin and angiotensin-converting enzyme activity. Indeed, they express renin, the renin receptor, angiotensinogen, and angiotensin-converting enzyme by mRNA analysis. Flow cytometric analysis and Western blot revealed angiotensin receptor 2 (AT(2)) expression in T and NK cells, whereas AT(1) expression was found in T and NK cells and monocytes by Western blot. These receptors were shown to be functional in calcium signaling, chemotaxis, and proliferation. However, AT, and AT2 antagonists alone or in combination were unable to abrogate completely the effects of AngII, suggesting that another AngII receptor may also be functional in leukocytes. This is the first study to show that T and NK cells are fully equipped with RAS elements and are potentially capable of producing and delivering AngII to sites of inflammation. Because their chemotaxis is enhanced by AngII, this creates a potential inflammatory amplification system. C1 Brigham & Womens Hosp, Div Renal, Transplantat Res Ctr, Boston, MA 02115 USA. NIAID, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. RP Abdi, R (reprint author), Brigham & Womens Hosp, Div Renal, Transplantat Res Ctr, 221 Longwood Ave, Boston, MA 02115 USA. EM rabdi@rics.bwh.harvard.edu OI McDermott, David/0000-0001-6978-0867; Tinckam, Kathryn/0000-0002-6638-2887 FU Intramural NIH HHS; NIAID NIH HHS [P01 AI50157] NR 50 TC 94 Z9 98 U1 1 U2 1 PU AMERICAN SOCIETY NEPHROLOGY PI WASHINGTON PA 1725 I ST, NW STE 510, WASHINGTON, DC 20006 USA SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD APR PY 2007 VL 18 IS 4 BP 1093 EP 1102 DI 10.1681/ASN.2006070707 PG 10 WC Urology & Nephrology SC Urology & Nephrology GA 154RE UT WOS:000245524300010 PM 17329576 ER PT J AU Xue, JL Eggers, PW Agodoa, LY Foley, RN Collins, AJ AF Xue, Jay L. Eggers, Paul W. Agodoa, Lawrence Y. Foley, Robert N. Collins, Allan J. TI Longitudinal study of racial and ethnic differences in developing end-stage renal disease among aged medicare beneficiaries SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Article ID CHRONIC KIDNEY-DISEASE; POTENTIAL EXPLANATORY FACTORS; GLOMERULAR-FILTRATION-RATE; UNITED-STATES; CARDIOVASCULAR-DISEASE; SOCIOECONOMIC-STATUS; EARLY INITIATION; NATIONAL-HEALTH; RISK-FACTORS; POPULATION AB Diabetes and hypertension are the leading causes of renal failure. This study investigated racial differences in developing ESRD by participants' diabetes and hypertension status. This longitudinal study included 1,306,825 Medicare beneficiaries who were aged >= 66 yr at the study start and followed up to 10 yr from January 1, 1993, for the development of ESRD or death. During the 10 yr, 0.93 patients per 100 received ESRD treatment. After adjustment for age and gender, among patients with diabetes, black patients were 2.4 to 2.7 times and other races/ethnicities 1.6 to 1.7 times more likely than white patients to develop ESRD. Among hypertensive patients, black patients were 2.5 to 2.9 and others 1.7 to 1.8 times more likely than white patients to develop ESRD. Among patients with neither diabetes nor hypertension, black patients were 3.5 and others 2.0 times more likely. Black men with diabetes were 1.9 to 2.1 and women 2.5 to 3.4 times more likely than their white counterparts to develop ESRD. Hypertensive black men were 2.1 to 2.2 and women 2.8 to 3.6 times more likely to develop ESRD. The same findings were noted in women of other races/ethnicities. Compared with white counterparts, mortality was higher for black patients in all cohorts but lower among patients with ESRD. Although they are leading causes for renal failure, diabetes and hypertension do not cause racial differences in developing ESRD. Minority women especially are at greater risk for ESRD than white women. Further studies are needed to determine whether earlier initiation of dialysis is a factor in higher ESRD incidence among minorities. C1 US Renal Data Syst, Coordinating Ctr, Minneapolis, MN 55404 USA. Univ Minnesota, Minneapolis, MN USA. NIDDK, NIH, Bethesda, MD USA. RP Xue, JL (reprint author), US Renal Data Syst, Coordinating Ctr, 914 S 8th St,Suite S-206, Minneapolis, MN 55404 USA. EM jxue@usrds.org FU NIDDK NIH HHS [N01-DK-9-2343] NR 38 TC 61 Z9 61 U1 0 U2 0 PU AMERICAN SOCIETY NEPHROLOGY PI WASHINGTON PA 1725 I ST, NW STE 510, WASHINGTON, DC 20006 USA SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD APR PY 2007 VL 18 IS 4 BP 1299 EP 1306 DI 10.1681/ASN.2006050524 PG 8 WC Urology & Nephrology SC Urology & Nephrology GA 154RE UT WOS:000245524300031 PM 17329578 ER PT J AU Teigen, PM AF Teigen, Philip M. TI Legislating fear and the public health in gilded age Massachusetts SO JOURNAL OF THE HISTORY OF MEDICINE AND ALLIED SCIENCES LA English DT Review DE public health; legislation; rabies; hydrophobia; zoonotic disease; dogs ID RABIES AB Between 1876 and 1881 Massachusetts experienced an outbreak of human rabies (hydrophobia). The entire state-the Governor, the legislature, the State Board of Health, newspapers, and the citizenry and elected officials of every town and city-reacted to the disease. Central to the response was the Commonwealth's legislature called the General Court. Through public hearings, their own debates, and the passage of legislation, it resolved widespread fear and anger, mediated conflicting concepts of disease, and promoted social solidarity in the face of an epidemic. This article first narrates the General Court's legislative actions; it then examines the conflicting understandings of disease causality; finally, it explores the social and political rituals the legislature drew upon to deal with this public health crisis. Arguing that public health legislation is simultaneously instrumental and symbolic, this article demonstrates that attention to both enriches the study of epidemics, historical and yet to come. C1 Natl Lib Med, Hist Med Div, Bethesda, MD 20894 USA. RP Teigen, PM (reprint author), Natl Lib Med, Hist Med Div, 8600 Rockville Pike, Bethesda, MD 20894 USA. EM pteigen@nih.gov NR 99 TC 2 Z9 2 U1 0 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-5045 J9 J HIST MED ALL SCI JI J. Hist. Med. Allied Sci. PD APR PY 2007 VL 62 IS 2 BP 141 EP 170 DI 10.1093/jhmas/jrl016 PG 30 WC Health Care Sciences & Services; History & Philosophy Of Science SC Health Care Sciences & Services; History & Philosophy of Science GA 149IS UT WOS:000245141200002 PM 16980330 ER PT J AU Logan, RA AF Logan, Robert A. TI Clinical, classroom, or personal education: attitudes about health literacy SO JOURNAL OF THE MEDICAL LIBRARY ASSOCIATION LA English DT Article ID MEDICINE; DISPARITIES AB Purpose: This study explores how diverse attitudes about health literacy are assessed by medical librarians and other health care professionals. Procedures: An online survey of thirty-six items was conducted using Q methodology in two phases in spring 2005 and winter 2006. Respondents (n = 51) were nonrandomly self-selected from a convenience sample of members of the Medical Library Association and a group of environmental health consultants to the National Library of Medicine. Findings: Three factors were identified. Factor I is optimistic and supportive of health literacy's transformative sociocultural and professional potential, if clinical settings become a launching point for health literacy activities. Factor 2 is less optimistic about health literacy's potential to improve clinical or patient outcomes and prefers to focus health literacy initiatives on classroom education settings. Factor 3 supports improving the nation's health literacy but tends to support health literacy initiatives when people privately interact with health information materials. Conclusions: Each factor's attitudes about the appropriate educational venue to initiate health literacy activities are different and somewhat mutually exclusive. This suggests that health literacy is seen through different perceptual frameworks that represent a possible source of professional disagreement. C1 Natl Lib Med, Lister Hill natl Ctr Biomed Commun, Bethesda, MD 20894 USA. RP Logan, RA (reprint author), Natl Lib Med, Lister Hill natl Ctr Biomed Commun, 8600 Rockville Pike,Bldg 38A,Room 9S914, Bethesda, MD 20894 USA. EM logan@nlm.nih.gov NR 29 TC 3 Z9 3 U1 1 U2 6 PU MEDICAL LIBRARY ASSOC PI CHICAGO PA 65 EAST WACKER PLACE, STE 1900, CHICAGO, IL 60601-7298 USA SN 1536-5050 J9 J MED LIBR ASSOC JI J. Med. Libr. Assoc. PD APR PY 2007 VL 95 IS 2 BP 127 EP 137 DI 10.3163/1536-5050.95.2.127 PG 11 WC Information Science & Library Science SC Information Science & Library Science GA 157BJ UT WOS:000245693300007 PM 17443245 ER PT J AU Lacroix, EM Collins, ME AF Lacroix, Eve-Marie Collins, Maria Elizabeth TI Interlibrary loan in US and Canadian health sciences libraries 2005: update on journal article use SO JOURNAL OF THE MEDICAL LIBRARY ASSOCIATION LA English DT Article AB Purpose: The authors analyzed 2.48 million interlibrary loan (ILL) requests entered in the National Library of Medicines (NLM's) DOCLINE system from 3,234 US and Canadian medical libraries during fiscal year (FY) 2005 to study their distribution and nature and the journals in which requested articles were published. Methods: Data from DOCLINE and NLM's indexing system and online catalog were used to analyze all DOCLINE ILL transactions acted on from October 2004 to September 2005. The authors compared results from this analysis to previous data collected in FY 1992. Results: Overall ILL volume in the United States and Canada is at about the same level as FY 1992 despite marked growth in online searching, knowledge discovery tools, and journals available online. Over 21,000 unique journal titles and 1.4 million unique articles were used to fill 2.2 million ILL requests in FY 2005. Over 1 million of the articles were requested only once by any network library. Fifty-two percent (11,022) of journals had 5 or fewer requests for articles from all the years of a journal by all libraries in the network. Fifty-two percent of the articles requested were published within the most recent 5 years. Conclusion: The overall ILL profile in the libraries studied has changed little since FY 1992, notable given other changes in publishing. Small changes, however, may reveal developing trends. Total ILL traffic has been declining in recent years following a peak in 2002, and fewer of the articles requested were published in the most recent five years compared to requests from 1992. C1 Natl Lib Med, Bethesda, MD 20894 USA. RP Lacroix, EM (reprint author), Natl Lib Med, 8600 Rockville Pike, Bethesda, MD 20894 USA. EM lacroixe@mail.nih.gov; collinm@mail.nih.gov NR 20 TC 3 Z9 3 U1 1 U2 2 PU MEDICAL LIBRARY ASSOC PI CHICAGO PA 65 EAST WACKER PLACE, STE 1900, CHICAGO, IL 60601-7298 USA SN 1536-5050 J9 J MED LIBR ASSOC JI J. Med. Libr. Assoc. PD APR PY 2007 VL 95 IS 2 BP 189 EP 194 DI 10.3163/1536-5050.95.2.189 PG 6 WC Information Science & Library Science SC Information Science & Library Science GA 157BJ UT WOS:000245693300014 PM 17443252 ER PT J AU Park, S Gallas, BD Badano, A Petrick, NA Myers, KJ AF Park, Subok Gallas, Bradon D. Badano, Aldo Petrick, Nicholas A. Myers, Kyle J. TI Efficiency of the human observer for detecting a Gaussian signal at a known location in non-Gaussian distributed lumpy backgrounds SO JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION LA English DT Article ID MODEL OBSERVERS; DETECTION TASKS; IMAGE QUALITY; PERFORMANCE; NOISE; MAMMOGRAMS AB A previous study [J. Opt. Soc. Am. A 22, 3 (2005)] has shown that human efficiency for detecting a Gaussian signal at a known location in non-Gaussian distributed lumpy backgrounds is approximately 4%. This human efficiency is much less than the reported 40% efficiency that has been documented for Gaussian-distributed lumpy backgrounds [J. Opt. Soc. Am. A 16, 694 (1999) and J. Opt. Soc. Am. A 18, 473 (2001)]. We conducted a psychophysical study with a number of changes, specifically in display-device calibration and data scaling, from the design of the aforementioned study. Human efficiency relative to the ideal observer was found again to be approximately 5%. Our variance analysis indicates that neither scaling nor display made a statistically significant difference. in human performance for the task. We conclude that the non-Gaussian distributed lumpy background is a major factor in our low human-efficiency results. (c) 2007 Optical Society of America. C1 US FDA, Ctr Devices & Radiol Hlth, Div Imaging & Appl Math,Lab Assessment Med Imagin, Natl Inst Biomed Imaging & Bioengn, Rockville, MD 20852 USA. RP Park, S (reprint author), US FDA, Ctr Devices & Radiol Hlth, Div Imaging & Appl Math,Lab Assessment Med Imagin, Natl Inst Biomed Imaging & Bioengn, Rockville, MD 20852 USA. EM subok.park@fda.hhs.gov OI Gallas, Brandon/0000-0001-7332-1620; badano, aldo/0000-0003-3712-6670 FU Intramural NIH HHS NR 26 TC 10 Z9 10 U1 0 U2 1 PU OPTICAL SOC AMER PI WASHINGTON PA 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA SN 1084-7529 J9 J OPT SOC AM A JI J. Opt. Soc. Am. A-Opt. Image Sci. Vis. PD APR PY 2007 VL 24 IS 4 BP 911 EP 921 DI 10.1364/JOSAA.24.000911 PG 11 WC Optics SC Optics GA 152EL UT WOS:000245343600002 PM 17361278 ER PT J AU Giger, J Davidhizar, RE Purnell, L Harden, JT Phillips, J Strickland, O AF Giger, Joyce Davidhizar, Ruth E. Purnell, Larry Harden, J. Taylor Phillips, Janice Strickland, Ora TI American Academy of Nursing Expert Panel report: Developing cultural competence to eliminate health disparities in ethnic minorities and other vulnerable populations SO JOURNAL OF TRANSCULTURAL NURSING LA English DT Editorial Material DE cultural competence; culture; health disparities; vulnerable populations ID CANCER-RESEARCH; WOMEN; CARE; ACCESS; RACE AB The members of the Expert Panel on Cultural Competence of the American Academy of Nursing (AAN) envisioned this article to serve as a catalyst to action by the Academy to take the lead in ensuring that measurable outcomes be achieved that reduce or eliminate health disparities commonly found among racial, ethnic, uninsured, underserved, and underrepresented populations residing throughout the United States. The purposes of this article are to (a) assess current issues related to closing the gap in health disparities and achieving cultural competence, (b) discuss a beginning plan of action from the Expert Panel on Cultural Competence for future endeavors and continued work in these areas beyond the 2002 annual conference on Closing the Gap in Health Disparities, and (c) provide clearly delineated recommendations to assist the Academy to plan strategies and to step forward in taking the lead in reshaping health care policies to eliminate health care and health disparities. C1 Univ Calif Los Angeles, Los Angeles, CA USA. Univ Delaware, Newark, DE 19716 USA. NIA, NIH, Bethesda, MD 20892 USA. Univ Chicago Hosp, Chicago, IL 60637 USA. Emory Univ, Atlanta, GA 30322 USA. RP Giger, J (reprint author), Univ Calif Los Angeles, Los Angeles, CA USA. NR 33 TC 48 Z9 50 U1 0 U2 5 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1043-6596 J9 J TRANSCULT NURS JI J. Transcult. Nurs. PD APR PY 2007 VL 18 IS 2 BP 95 EP 102 DI 10.1177/1043659606298618 PG 8 WC Nursing SC Nursing GA 147DF UT WOS:000244982900002 PM 17416710 ER PT J AU Espinoza, J Gotsch, F Kusanovic, JP Goncalves, LF Lee, W Hassan, S Mittal, P Schoen, ML Romero, R AF Espinoza, Jimmy Gotsch, Francesca Kusanovic, Juan Pedro Goncalves, Luis F. Lee, Wesley Hassan, Sonia Mittal, Pooja Schoen, Mary Lou Romero, Roberto TI Changes in fetal cardiac geometry with gestation - Implications for 3-and 4-dimensional fetal echocardiography SO JOURNAL OF ULTRASOUND IN MEDICINE LA English DT Article DE algorithm; cardiac geometry; morphogenesis; 3-dimensional; 4-dimensional; ultrasonography ID SPATIOTEMPORAL IMAGE CORRELATION; CONGENITAL HEART-DISEASE; LEFT-RIGHT ASYMMETRY; ULTRASONOGRAPHY; ULTRASOUND; DISPLAY; DIMENSIONS; DEFECTS; ANATOMY; FIELD/ AB Objective. Three- and 4-dimensional fetal echocardiography can be performed using novel algorithms. However, these algorithms assume that the spatial relationships among cardiac chambers and great vessels are constant throughout gestation. The objective of this study was to determine whether changes in fetal cardiac geometry occur during gestation. Methods. A cross-sectional study was conducted by reviewing 3- and 4-dimensional volume data sets from healthy fetuses obtained between 12 and 41 weeks of gestation, Volume data sets were examined using commercially available software. Parameters measured included angles between: (1) the ductal arch and fetal thoracic aorta; (2) the ductal arch and aortic arch; and (3) the left outflow tract and main pulmonary artery, as seen in the short axis of the heart. The mean angle from the left outflow tract to the short axis was calculated. Nonparametric statistics were used for analysis. Results. Eighty-five fetuses were included in the study. The angle between the ductal arch and the fetal thoracic aorta decreased with gestational age (Spearman rho coefficient: -0.39; P < .001). In contrast, the angle between the ductal arch and aortic arch, and the mean angle between the left outflow tract and the short axis of the heart increased with gestational age (Spearman p coefficients: 0.45 and 0.40, respectively; P < .001). Conclusions. (1) Changes in fetal cardiac geometry were shown with advancing gestational age. (2) Proposed algorithms for the examination of the fetal heart with 3-dimensional ultrasonography may need to be adapted to optimize visualization of the standard planes before 26 weeks of gestation. C1 Wayne State Univ, Hutzel Womens Hosp, NICHD, Perinatol Res Branch,NIH,DHHS, Detroit, MI 48201 USA. Wayne State Univ, Hutzel Womens Hosp, Dept Obstet & Gynecol, Detroit, MI 48201 USA. William Beaumont Hosp, Div Fetal Imaging, Royal Oak, MI 48072 USA. Wayne State Univ, Ctr Mol Med & Genet, Detroit, MI USA. RP Romero, R (reprint author), Wayne State Univ, Hutzel Womens Hosp, NICHD, Perinatol Res Branch,NIH,DHHS, 3990 John R,Box 4, Detroit, MI 48201 USA. EM warfiela@mail.nih.gov FU Intramural NIH HHS [Z01 HD002401-15, Z99 HD999999] NR 29 TC 5 Z9 8 U1 0 U2 0 PU AMER INST ULTRASOUND MEDICINE PI LAUREL PA SUBSCRIPTION DEPT, 14750 SWEITZER LANE, STE 100, LAUREL, MD 20707-5906 USA SN 0278-4297 J9 J ULTRAS MED JI J. Ultrasound Med. PD APR PY 2007 VL 26 IS 4 BP 437 EP 443 PG 7 WC Acoustics; Radiology, Nuclear Medicine & Medical Imaging SC Acoustics; Radiology, Nuclear Medicine & Medical Imaging GA 149SH UT WOS:000245166400003 PM 17384040 ER PT J AU Graff, JN Mori, M Li, H Garzotto, M Penson, D Potosky, AL Beer, TM AF Graff, Julie N. Mori, Motomi Li, Hong Garzotto, Mark Penson, David Potosky, Arnold L. Beer, Tomasz M. TI Predictors of overall and cancer-free survival of patients with localized prostate cancer treated with primary androgen suppression therapy: Results from the prostate cancer outcomes study SO JOURNAL OF UROLOGY LA English DT Article DE prostate; prostatic neoplasms; androgen antagonists; mortality; nomograms ID QUALITY-OF-LIFE; RADICAL PROSTATECTOMY; DEPRIVATION THERAPY; HORMONAL-THERAPY; RADIATION-THERAPY; PRACTICE PATTERNS; CARCINOMA; TRIAL; MEN AB Purpose: Primary androgen suppression therapy for clinically localized prostate cancer is increasingly common in the United States despite a lack of supportive evidence for its use. We determined which demographic and clinical factors predict overall and cancer specific survival with this treatment strategy in patients enrolled in the Prostate Cancer Outcomes Study. Materials and Methods: In 1994 to 1995 the Prostate Cancer Outcomes Study recruited 3,533 men diagnosed with prostate cancer. Clinical and treatment information was abstracted from medical records and demographic characteristics were obtained from patient surveys 6, 12, 24 and 60 months after diagnosis. Overall and cancer specific mortality was analyzed through December 2002 using the Kaplan-Meier method and Cox regression. Results: A total of 276 patients had organ confined (cTl-2) prostatic adenocarcinoma and received primary androgen suppression therapy within 1 year of diagnosis. Median followup for censored patients was 7.6 years (range 1.1 to 8.1). Five-year overall and cancer specific survival was 66% (95% CI 59-72) and 91% (95% CI 86-94), respectively. Independent predictors of shorter overall survival were patient age 75 years or older, prostate specific antigen 20 ng/ml or greater, Gleason score 7 or greater and abnormal digital rectal examination. Gleason score 7 or greater, prostate specific antigen 20 ng/ml or greater and a low comorbidity index were independent predictors of shorter cancer specific survival. Conclusions: The use of primary androgen suppression therapy in the Prostate Cancer Outcomes Study data set resulted in 91% 5-year cancer specific survival. Advanced age, and factors that reflect tumor burden and biology were predictive of overall survival, while cancer specific survival was predicted by tumor factors and the burden of comorbid conditions. A nomogram for predicting overall survival at 5 years was constructed. C1 Oregon Hlth & Sci Univ, Dept Med, Div Hematol & Med Oncol, Portland, OR 97239 USA. Oregon Hlth & Sci Univ, Div Urol, Portland, OR 97239 USA. Oregon Hlth & Sci Univ, Biostat Shared Resource, Inst Canc, Portland, OR 97239 USA. Portland VA Med Ctr, Urol Sect, Portland, OR USA. Univ So Calif, Dept Urol, Norris Canc Ctr, Los Angeles, CA 90089 USA. Univ So Calif, Dept Prevent Med, Norris Canc Ctr, Los Angeles, CA 90089 USA. NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. RP Beer, TM (reprint author), Oregon Hlth & Sci Univ, Dept Med, Div Hematol & Med Oncol, Mail Code CR-145,3181 SW Sam Jackson Pk Rd, Portland, OR 97239 USA. EM beert@ohsu.edu FU NCI NIH HHS [N01PC67010, N01PC67009, N01PC67005, N01PC67006, N01PC67000, N01PC67007] NR 20 TC 12 Z9 12 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-5347 J9 J UROLOGY JI J. Urol. PD APR PY 2007 VL 177 IS 4 BP 1307 EP 1312 DI 10.1016/j.juro.2006.11.054 PG 6 WC Urology & Nephrology SC Urology & Nephrology GA 148QV UT WOS:000245090600030 PM 17382720 ER PT J AU Clemens, JQ Link, CL Eggers, PW Kusek, JW Nyberg, LM McKinlay, JB AF Clemens, J. Quentin Link, Carol L. Eggers, Paul W. Kusek, John W. Nyberg, Leroy M., Jr. McKinlay, John B. CA BACH Survey Investigators TI Prevalence of painful bladder symptoms and effect on quality of life in black, Hispanic and white men and women SO JOURNAL OF UROLOGY LA English DT Article DE bladder; cystitis; interstitial; epidemiology; minority groups; continental population groups ID INTERSTITIAL CYSTITIS SYMPTOMS; BENIGN PROSTATIC HYPERPLASIA; MANAGED CARE POPULATION; CHRONIC PELVIC PAIN; HEALTH; INDEX AB Purpose: The prevalence of painful bladder symptoms is poorly defined, especially in racial and ethnic minority groups. We estimated the prevalence of painful bladder symptoms in a community based sample, assessed symptom variation by age, gender, race/ethnicity and socioeconomic status, and estimated their impact on quality of life. Materials and Methods: A population based cross-sectional survey of individuals was done in the Boston area using a multistage stratified cluster sample. A 2-hour in person interview performed by a bilingual interviewer was done, generally in the home of the subject. The research design was for equal numbers of subjects in each of 24 design cells, as defined by age (30 to 39, 40 to 49, 50 to 59 and 60 to 79 years), gender and race/ethnicity (black, Hispanic and white). The sample of 5,506 subjects was recruited from April 2002 through June 2005. Multiple definitions of painful bladder symptoms were used based on consensus statements, research definitions and published articles. The effect of gender, age, race/ethnicity and socioeconomic status on symptom prevalence was assessed. Results: The prevalence of painful bladder syndrome symptoms was 0.83% to 2.71% in women and 0.25% to 1.22% in men depending on the definition used. Multivariate analyses revealed that symptoms were significantly more common in women, middle-aged individuals (40 to 59 years old) and lower socioeconomic status groups. For most definitions there were no variations by race or ethnicity. The presence of symptoms was associated with a significant adverse impact on quality of life. Conclusions: Painful bladder symptoms are more common than suggested by coded physician diagnoses. Although most bladder pain research cohorts have included predominantly white women, these population based findings indicate no racial/ethnic disparity and limited gender disparity in the prevalence of painful bladder symptoms. This suggests that the burden of painful bladder syndrome may be greater in nonwhite individuals and men than previously suspected. C1 Northwestern Univ, Dept Urol, Feinberg Sch Med, Chicago, IL 60611 USA. New England Res Inst, Watertown, MA 02172 USA. NIDDKD, Bethesda, MD 20892 USA. RP Clemens, JQ (reprint author), Northwestern Univ, Dept Urol, Feinberg Sch Med, 303 E Chicago Ave,Tarry 16-703, Chicago, IL 60611 USA. EM qclemens@northwestern.edu FU NIDDK NIH HHS [U01 DK 56842] NR 23 TC 57 Z9 59 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-5347 J9 J UROLOGY JI J. Urol. PD APR PY 2007 VL 177 IS 4 BP 1390 EP 1394 DI 10.1016/j.juro.2006.11.084 PG 5 WC Urology & Nephrology SC Urology & Nephrology GA 148QV UT WOS:000245090600049 PM 17382739 ER PT J AU Weber, AM AF Weber, Anne M. TI The role of provider volume on outcomes after sling surgery for stress urinary incontinence - Editorial comment SO JOURNAL OF UROLOGY LA English DT Editorial Material C1 NICHHD, Female Pelv Floor Disorders Ctr Populat Res, NIH, Bethesda, MD 20892 USA. RP Weber, AM (reprint author), NICHHD, Female Pelv Floor Disorders Ctr Populat Res, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-5347 J9 J UROLOGY JI J. Urol. PD APR PY 2007 VL 177 IS 4 BP 1462 EP 1462 DI 10.1016/j.juro.2006.11.160 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA 148QV UT WOS:000245090600063 ER PT J AU Surman, SR Collins, PL Murphy, BR Skiadopoulos, MH AF Surman, Sonja R. Collins, Peter L. Murphy, Brian R. Skiadopoulos, Mario H. TI An improved method for the recovery of recombinant paramyxovirus vaccine candidates suitable for use in human clinical trials SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE paramyxovirus; reverse-genetics; clinical trials; electroporation ID REVERSE GENETICS; MUTATIONS; REPLICATION; MONKEYS AB We describe a method for the generation of clinical grade, live-attenuated vaccines in Vero cells entirely from cDNA plasmids. The entire electroporation procedure can be completed in less than 15 minutes and this is a significant improvement over previous lipid or electroporation based transfection techniques that also involve a heat-shock step. Importantly, the virus preparations can be generated with a minimal use of animal product derived materials, an important consideration for a vaccine candidate that is to be tested in humans. Since it is likely that all live-attenuated parainfluenza virus and pneumovirus vaccines in the future will be generated using reverse genetics, this simplified method provides guidance on how this can be achieved. (c) 2006 Elsevier B.V. All rights reserved. C1 NIAID, Lab Infect Dis, Resp Viruses Sect, NIH, Bethesda, MD 20892 USA. RP Skiadopoulos, MH (reprint author), NIAID, Lab Infect Dis, Resp Viruses Sect, NIH, 50 S Dr,Bldg 50,Room 6511,MSC 8007, Bethesda, MD 20892 USA. EM mskiadopoulos@niaid.nih.gov FU Intramural NIH HHS NR 10 TC 11 Z9 11 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 J9 J VIROL METHODS JI J. Virol. Methods PD APR PY 2007 VL 141 IS 1 BP 30 EP 33 DI 10.1016/j.jviromet.2006.11.024 PG 4 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA 149MD UT WOS:000245150100005 PM 17210187 ER PT J AU Demberg, T Florese, RH Heath, MJ Larsen, K Kalisz, I Kalyanaraman, VS Lee, EM Pal, R Venzon, D Grant, R Patterson, LJ Korioth-Schmitz, B Buzby, A Dombagoda, D Montefiori, DC Letvin, NL Cafaro, A Ensoli, B Robert-Guroff, M AF Demberg, Thorsten Florese, Ruth H. Heath, Megan J. Larsen, Kay Kalisz, Irene Kalyanaraman, V. S. Lee, Eun Mi Pal, Ranajit Venzon, David Grant, Richard Patterson, L. Jean Korioth-Schmitz, Birgit Buzby, Adam Dombagoda, Dilani Montefiori, David C. Letvin, Norman L. Cafaro, Aurelio Ensoli, Barbara Robert-Guroff, Marjorie TI A replication-competent adenovirus-human immunodeficiency virus (Ad-HIV) tat and Ad-HIV env priming/Tat and envelope protein boosting regimen elicits enhanced protective efficacy against simian/human immunodeficiency virus SHIV89.6P challenge in rhesus macaques SO JOURNAL OF VIROLOGY LA English DT Article ID DEPENDENT CELLULAR CYTOTOXICITY; IMMUNE-RESPONSES; DENDRITIC CELLS; T-CELLS; NEUTRALIZING ANTIBODIES; VACCINE DEVELOPMENT; CYNOMOLGUS MONKEYS; 89.6P CHALLENGE; UP-REGULATION; INFECTION AB We previously demonstrated that replication-competent adenovirus (Ad)-simian immunodeficiency virus (SIV) recombinant prime/protein boost regimens elicit potent immunogenicity and strong, durable protection of rhesus macaques against SIVmac251. Additionally, native Tat vaccines have conferred strong protection against simian/human immunodeficiency virus SHIV89.6P challenge of cynomolgus monkeys, while native, inactivated, or vectored Tat vaccines have failed to elicit similar protective efficacy in rhesus macaques. Here we asked if priming rhesus macaques with replicating Ad-human immunodeficiency virus (HIV) tat and boosting with the Tat protein would elicit protection against SHIV89.6P. We also evaluated a Tat/Env regimen, adding an Ad-HIV env recombinant and envelope protein boost to test whether envelope antibodies would augment acute-phase protection. Further, expecting cellular immunity to enhance chronic viremia control, we tested a multigenic group: Ad-HIV tat, -HIV env, -SIV gag, and -SIV nef recombinants and Tat, Env, and Nef proteins. All regimens were immunogenic. A hierarchy was observed in enzyme-linked immunospot responses (with the strongest response for Env, followed by Gag, followed by Nef, followed by Tat) and antibody titers (with the highest titer for Env, followed by Tat, followed by Nef, followed by Gag). Following intravenous SHIV89.6P challenge, all macaques became infected. Compared to controls, no protection was seen in the Tat-only group, confirming previous reports for rhesus macaques. However, the multigenic group blunted acute viremia by approximately 1 log (P = 0.017), and both the multigenic and Tat/Env groups reduced chronic viremia by 3 and 4 logs, respectively, compared to controls (multigenic, P = 0.0003; Tat/Env, P < 0.0001). The strikingly greater reduction in the Tat/Env group than in the multigenic group (P = 0.014) was correlated with Tat and Env binding antibodies. Since prechallenge anti-Env antibodies lacked SHIV89.6P-neutralizing activity, other functional anti-Env and anti-Tat activities are under investigation, as is a possible synergy between the Tat and Env immunogens. C1 NCI, Vaccine Branch, NIH, Bethesda, MD 20892 USA. Univ Washington, Natl Primate Res Ctr, Seattle, WA 98195 USA. Adv BioSci Labs Inc, Kensington, MD 20895 USA. NCI, Biostat & Data Management Sect, Bethesda, MD 20892 USA. Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Boston, MA 02115 USA. Duke Univ, Med Ctr, Durham, NC 27710 USA. Ist Super Sanita, Natl AIDS Ctr, I-00161 Rome, Italy. RP Robert-Guroff, M (reprint author), NCI, Vaccine Branch, NIH, 41 Medlars Dr,Bldg 41,Room D804, Bethesda, MD 20892 USA. EM guroffm@mail.nih.gov RI Ensoli, Barbara/J-9169-2016; Cafaro, Aurelio/K-5314-2016; Korioth-Schmitz, Birgit/M-7816-2015 OI Ensoli, Barbara/0000-0002-0545-8737; Korioth-Schmitz, Birgit/0000-0002-5271-9223 FU Intramural NIH HHS; NIAID NIH HHS [N01 AI 15431, N01AI15431] NR 60 TC 59 Z9 61 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2007 VL 81 IS 7 BP 3414 EP 3427 DI 10.1128/JVI.02453-06 PG 14 WC Virology SC Virology GA 147FF UT WOS:000244988100037 PM 17229693 ER PT J AU Cocklin, S Gopi, H Querido, B Nimmagadda, M Kuriakose, S Cicala, C Ajith, S Baxter, S Arthos, J Martin-Garcia, J Chaiken, IA AF Cocklin, Simon Gopi, Hosahudya Querido, Bianca Nimmagadda, Manideepthi Kuriakose, Syna Cicala, Claudia Ajith, Sandya Baxter, Sabine Arthos, James Martin-Garcia, Julio Chaiken, Irwin A. TI Broad-spectrum anti-human immunodeficiency virus (HIV) potential of a peptide HIV type 1 entry inhibitor SO JOURNAL OF VIROLOGY LA English DT Article ID MONOCLONAL-ANTIBODIES; ENVELOPE GLYCOPROTEIN; FUSION INHIBITOR; GP120 CORE; NEUTRALIZATION; MICROGLIA; RECEPTOR; ENFUVIRTIDE; MACROPHAGES; MECHANISMS AB The AIDS epidemic continues to spread at an alarming rate worldwide, especially in developing countries. One approach to solving this problem is the generation of anti-human immunodeficiency virus (HIV) compounds with inhibition spectra broad enough to include globally prevailing forms of the virus. We have examined the HIV type 1 (HIV-1) envelope specificity of a recently identified entry inhibitor candidate, UNG-105, using surface plasmon resonance spectroscopy and pseudovirus inhibition assays. The combined results suggest that the HNG-105 molecule may be effective across the HIV-1 subtypes, and they highlight its potential as a lead for developing therapeutic and microbicidal agents to help combat the spread of AIDS. C1 Drexel Univ, Coll Med, Dept Biochem & Mol Biol, Philadelphia, PA 19102 USA. Drexel Univ, Coll Med, Dept Microbiol & Immunol, Ctr Mol Virol & Neuroimmunol,Inst Mol Med & Infec, Philadelphia, PA 19102 USA. NIAID, Immunoregulat Lab, NIH, Bethesda, MD 20892 USA. RP Cocklin, S (reprint author), Drexel Univ, Coll Med, Dept Biochem & Mol Biol, 11313 New Coll Bldg,MS 497,245 N 15th St,245 N 15, Philadelphia, PA 19102 USA. EM sc349@drexel.edu RI Martin-Garcia, Julio/A-5666-2009 OI Martin-Garcia, Julio/0000-0001-5450-5029 FU NIAID NIH HHS [R21 AI 071265-01]; NIGMS NIH HHS [P01 GM 056550-08, P01 GM056550]; NINDS NIH HHS [R21 NS047970] NR 34 TC 21 Z9 23 U1 2 U2 6 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2007 VL 81 IS 7 BP 3645 EP 3648 DI 10.1128/JVI.01778-06 PG 4 WC Virology SC Virology GA 147FF UT WOS:000244988100061 PM 17251295 ER PT J AU Kiselyeva, Y Nedellec, R Ramos, A Pastore, C Margolis, LB Mosier, DE AF Kiselyeva, Yana Nedellec, Rebecca Ramos, Alejandra Pastore, Cristina Margolis, Leonid B. Mosier, Donald E. TI Evolution of CXCR4-using human immunodeficiency virus type 1 SF162 is associated with two unique envelope mutations SO JOURNAL OF VIROLOGY LA English DT Article ID SYNCYTIUM-INDUCING PHENOTYPE; HUMAN LYMPHOID-TISSUE; T-CELL-LINE; V3 LOOP; MACROPHAGE-TROPISM; SMALL-MOLECULE; BIOLOGICAL PHENOTYPE; CORECEPTOR USAGE; ENTRY INHIBITOR; INFECTION AB CCR5-using human immunodeficiency virus type 1 (HIV-1) isolates typically gain CXCR4 use via multiple mutations in V3 and often V1/V2 regions of envelope, and patterns of mutations are distinct for each isolate. Here, we report that multiple CXCR4-using variants of a parental CCR5-using HIV-1 isolate, SF162, obtained by either target cell selection or CCR5 inhibition have a common mutation pattern characterized by the same two V3 mutations and that these mutations preexisted in some of the SF162 stocks. These results imply that SF162 has a single pathway for acquiring CXCR4 use and that prolonged culture is sufficient to select for R5X4 variants. C1 NICHD, Lab Mol & Cellular Biophys, NIH, Bethesda, MD 20892 USA. Scripps Res Inst, Dept Immunol, La Jolla, CA 92037 USA. RP Margolis, LB (reprint author), NICHD, Lab Mol & Cellular Biophys, NIH, 10 Ctr Dr,10-9D58, Bethesda, MD 20892 USA. EM margolis@helix.nih.gov; dmosier@scripps.edu FU Intramural NIH HHS; NIAID NIH HHS [R01 AI052778, R56 AI052778, AI 52778] NR 39 TC 10 Z9 10 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2007 VL 81 IS 7 BP 3657 EP 3661 DI 10.1128/JVI.02310-06 PG 5 WC Virology SC Virology GA 147FF UT WOS:000244988100064 PM 17202224 ER PT J AU Chaudhuri, R Lindwasser, OW Smith, WJ Hurley, JH Bonifacino, JS AF Chaudhuri, Rittik Lindwasser, O. Wolf Smith, William J. Hurley, James H. Bonifacino, Juan S. TI Downregulation of CD4 by human immunodeficiency virus type 1 Nef is dependent on clathrin and involves direct interaction of Nef with the AP2 clathrin adaptor SO JOURNAL OF VIROLOGY LA English DT Article ID CELL-SURFACE CD4; CLASS-I MHC; LONG-TERM SURVIVOR; WIDE RNAI SCREEN; HIV-1 NEF; ENDOCYTIC PATHWAY; SORTING SIGNALS; DILEUCINE MOTIF; BETA-COP; PHYSICAL INTERACTION AB Nef, an accessory protein of human and simian immunodeficiency viruses, is a critical determinant of pathogenesis that promotes the progression from infection to AIDS. The pathogenic effects of Nef are in large part dependent on its ability to downregulate the macrophage and T-cell coreceptor, CD4. It has been proposed that Nef induces downregulation by linking the cytosolic tail of CD4 to components of the host-cell protein trafficking machinery. To identify these components, we developed a novel Nef-CD4 downregulation system in Drosophila melanogaster S2 cells. We found that human immunodeficiency virus type 1 (HIV-1) Nef downregulates human CD4 in S2 cells and that this process is subject to the same sequence requirements as in human cells. An RNA interference screen targeting protein trafficking genes in S2 cells revealed a requirement for clathrin and the clathrin-associated, plasma membrane-localized AP2 complex in the downregulation of CD4. The requirement for AP2 was confirmed in the human cell line HeLa. We also used a yeast three-hybrid system and glutathione S-transferase pull-down analyses to demonstrate a robust, direct interaction between HIV-1 Nef and AP2. This interaction requires a dileucine motif in Nef that is also essential for downregulation of CD4. Together, these results support a model in which HIV-1 Nef downregulates CD4 by promoting its accelerated endocytosis by a clathrin/AP2 pathway. C1 NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA. NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. RP Bonifacino, JS (reprint author), NICHHD, Cell Biol & Metab Branch, NIH, Bldg 18T,Rm 101, Bethesda, MD 20892 USA. EM juan@helix.nih.gov OI Bonifacino, Juan S./0000-0002-5673-6370 FU Intramural NIH HHS; NICHD NIH HHS [K22 HD54602] NR 81 TC 108 Z9 110 U1 0 U2 6 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2007 VL 81 IS 8 BP 3877 EP 3890 DI 10.1128/JVI.02725-06 PG 14 WC Virology SC Virology GA 157BF UT WOS:000245692900021 PM 17267500 ER PT J AU Wang, YH Dennehy, PH Keyserling, HL Tang, K Gentsch, JR Glass, RI Jiang, BM AF Wang, Yuhuan Dennehy, Penelope H. Keyserling, Harry L. Tang, Kevin Gentsch, Jon R. Glass, Roger I. Jiang, Baoming TI Rotavirus infection alters peripheral T-cell homeostasis in children with acute diarrhea SO JOURNAL OF VIROLOGY LA English DT Article ID B-CELLS; TRANSCRIPTION FACTOR; RHESUS ROTAVIRUS; CUTTING EDGE; VP6 PROTEIN; EXPRESSION; DISEASE; LYMPHOCYTES; CYTOKINE; IMMUNITY AB The patterns of gene expression and the phenotypes of lymphocytes in peripheral blood mononuclear cells (PBMC) from children with diarrhea caused by rotavirus and healthy children were compared by using DNA microarray, quantitative PCR, and flow cytometry. We observed increased expression of a number of genes encoding proinflammatory cytokines and interferon or interferon-stimulated proteins and demonstrated activation of some genes involved in the differentiation, maturation, activation, and survival of B lymphocytes in PBMC of patients with rotavirus infection. In contrast, we observed a consistent pattern of lower mRNA levels for an array of genes involved in the various stages of T-cell development and demonstrated a reduction in total lymphocyte populations and in the proportions of CD4 and CD8 T lymphocytes from PBMC of patients. This decreased frequency of T lymphocytes was transient, since the proportions of T lymphocytes recovered to almost normal levels in convalescent-phase PBMC from most patients. Finally, rotavirus infection induced the activation and expression of the early activation markers CD83 and CD69 on a fraction of CD19 B cells and the remaining CD4 and CD8 T lymphocytes in acute-phase PBMC of patients; the expression of CD83 continued to be elevated and was predominantly exhibited on CD4 T lymphocytes in convalescent-phase PBMC. On the basis of these findings at the molecular, phenotypic, and physiologic levels in acute-phase PBMC, we conclude that rotavirus infection induces robust proinflammatory and antiviral responses and B-cell activation but alters peripheral T-cell homeostasis in children. C1 Emory Univ, Sch Med, Div Viral Dis, Atlanta, GA 30322 USA. Emory Univ, Sch Med, Sci Resources Program, Atlanta, GA 30322 USA. Emory Univ, Sch Med, Ctr Dis Control & Prevent, Atlanta, GA 30322 USA. Emory Univ, Sch Med, Dept Pediat, Atlanta, GA 30322 USA. Rhode Isl Hosp, Div Pediat Infect Dis, Providence, RI USA. Fogarty Int Ctr, NIH, Bethesda, MD USA. RP Jiang, BM (reprint author), Natl Ctr IMmunizat & Resp Dis, Gastroenteritis & Resp Viruses Branch, MS G04,1600 Clifton Rd, Atlanta, GA 30333 USA. EM bjiang@cdc.gov OI Dennehy, Penelope/0000-0002-2259-5370 NR 56 TC 31 Z9 32 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2007 VL 81 IS 8 BP 3904 EP 3912 DI 10.1128/JVI.01887-06 PG 9 WC Virology SC Virology GA 157BF UT WOS:000245692900023 PM 17267507 ER PT J AU Moore, MD Fu, W Nikolaitchik, O Chen, JB Ptak, RG Hu, WS AF Moore, Michael D. Fu, William Nikolaitchik, Olga Chen, Jianbo Ptak, Roger G. Hu, Wei-Shau TI Dimer initiation signal of human immunodeficiency virus type 1: Its role in partner selection during RNA copackaging and its effects on recombination SO JOURNAL OF VIROLOGY LA English DT Article ID MURINE LEUKEMIA-VIRUS; VIRAL REPLICATION; HIV-1 RNA; IN-VITRO; MEDIATED DIMERIZATION; GENETIC-RECOMBINATION; PARTICLE MATURATION; KISSING COMPLEX; STABLE DIMER; GENOMIC RNA AB Frequent human immunodeficiency virus type I (HIV-1) recombination occurs during DNA synthesis when portions of the two copackaged RNAs are used as templates to generate a hybrid DNA copy. Therefore, the frequency of copackaging of genomic RNAs from two different viruses (heterozygous virion formation) affects the generation of genotypically different recombinants. We hypothesized that the selection of copackaged RNA partners is largely determined by Watson-Crick pairing at the dimer initiation signal (DIS), a 6-nucleotide palindromic sequence at the terminal loop of stem-loop I (SM). To test our hypothesis, we examined whether heterozygous virion formation could be encouraged by manipulation of the DIS. Three pairs of viruses were generated with compensatory DIS mutations, designed so that perfect DIS base pairing could only occur between RNAs derived from different viruses, not between RNAs from the same virus. We observed that vector pairs with compensatory DIS mutations had an almost twofold increase in recombination rates compared with wild-type viruses. These data suggest that heterozygous virion formation was enhanced in viruses with compensatory DIS mutations (from 50% to more than 90% in some viral pairings). The role of the SL1 stem in heterozygous virion formation was also tested; our results indicated that the intermolecular base pairing of the stem sequences does not affect RNA partner selection. In summary, our results demonstrate that the Watson-Crick pairing of the DIS is a major determinant in the selection of the copackaged RNA partner, and altering the base pairing of the DIS can change the proportion of heterozygous viruses in a viral population. These results also strongly support the hypothesis that HIV-1 RNA dimers are formed prior to encapsidation. C1 NCI Frederick, HIV Drug Resistance Program, Ft Detrick, MD 21702 USA. So Res Inst, Ft Detrick, MD 21702 USA. RP Hu, WS (reprint author), NCI Frederick, HIV Drug Resistance Program, POB B,Bldg 535,Room 336, Ft Detrick, MD 21702 USA. EM whu@ncifcrf.gov RI Chen, Jianbo/N-3737-2014 OI Chen, Jianbo/0000-0001-6491-6577 FU Intramural NIH HHS NR 33 TC 63 Z9 64 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2007 VL 81 IS 8 BP 4002 EP 4011 DI 10.1128/JVI.02589-06 PG 10 WC Virology SC Virology GA 157BF UT WOS:000245692900032 PM 17267488 ER PT J AU Raymond, GJ Raymond, LD Meade-White, KD Hughson, AG Favara, C Gardner, D Williams, ES Miller, MW Race, RE Caughey, B AF Raymond, Gregory J. Raymond, Lynne D. Meade-White, Kimberly D. Hughson, Andrew G. Favara, Cynthia Gardner, Donald Williams, Elizabeth S. Miller, Michael W. Race, Richard E. Caughey, Byron TI Transmission and adaptation of chronic wasting disease to hamsters and transgenic mice: Evidence for strains SO JOURNAL OF VIROLOGY LA English DT Article ID RESISTANT PRION PROTEIN; MINK ENCEPHALOPATHY; MULE DEER; SCRAPIE; PRP; ELK; CONVERSION; BARRIER; HUMANS; CATTLE AB In vitro screening using the cell-free prion protein conversion system indicated that certain rodents may be susceptible to chronic wasting disease (CWD). Therefore, CWD isolates from mule deer, white-tailed deer, and elk were inoculated intracerebrally into various rodent species to assess the rodents' susceptibility and to develop new rodent models of CWD. The species inoculated were Syrian golden, Djungarian, Chinese, Siberian, and Armenian hamsters, transgenic mice expressing the Syrian golden hamster prion protein, and RML Swiss and C57BL10 wild-type mice. The transgenic mice and the Syrian golden, Chinese, Siberian, and Armenian hamsters had limited susceptibility to certain of the CWD inocula, as evidenced by incomplete attack rates and long incubation periods. For serial passages of CWD isolates in Syrian golden hamsters, incubation periods rapidly stabilized, with isolates having either short (85 to 89 days) or long (408 to 544 days) mean incubation periods and distinct neuropathological patterns. In contrast, wild-type mouse strains and Djungarian hamsters were not susceptible to CWD. These results show that CWD can be transmitted and adapted to some species of rodents and suggest that the cervid-derived CWD inocula may have contained or diverged into at least two distinct transmissible spongiform encephalopathy strains. C1 Rocky Mt Labs, NIAID, NIH, Hamilton, MT 59840 USA. Rocky Mt Labs, Lab Persistent Viral Dis, Hamilton, MT 59840 USA. Rocky Mt Labs, Rocky Mt Vet Branch, Hamilton, MT 59840 USA. Univ Wyoming, Dept Vet Sci, Laramie, WY 82070 USA. Wildlife Res Ctr, Colorado Div Wildlife, Ft Collins, CO 80526 USA. RP Race, RE (reprint author), Rocky Mt Labs, NIAID, NIH, 903 S 4th St, Hamilton, MT 59840 USA. EM rrace@nih.gov; bcaughey@nih.gov FU Intramural NIH HHS NR 25 TC 47 Z9 49 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2007 VL 81 IS 8 BP 4305 EP 4314 DI 10.1128/JVI.02474-06 PG 10 WC Virology SC Virology GA 157BF UT WOS:000245692900061 PM 17287284 ER PT J AU Thomas, JA Ott, DE Gorelick, RJ AF Thomas, James A. Ott, David E. Gorelick, Robert J. TI Efficiency of human immunodeficiency virus type 1 postentry infection processes: Evidence against disproportionate numbers of defective virions SO JOURNAL OF VIROLOGY LA English DT Article ID VESICULAR STOMATITIS-VIRUS; MURINE LEUKEMIA-VIRUS; NUCLEOCAPSID PROTEIN; ENVELOPE GLYCOPROTEINS; REPLICATION-COMPETENT; REVERSE TRANSCRIPTION; RETROVIRAL VECTORS; GENE-EXPRESSION; HIV-1 PARTICLES; ZN2+ FINGERS AB The vast majority of human immunodeficiency virus type I particles are claimed to be noninfectious, but there is disagreement as to whether they are defective or simply lack the opportunity to initiate an infection. We have examined the efficiencies of reverse transcription and integration and find that approximately 1 of every 8 virions that initiate reverse transcription form proviruses, a quantity significantly different from the commonly reported ratio of I in 1,000. In addition, results from two different infectivity assays demonstrate that the titers are not equivalent to the number of infectious particles. The apparent predominance of noninfectious particles is due to infrequent occurrences of successful virus-cell interactions. C1 SAIC Frederick Inc, Natl Canc Inst, Basic Res Program, AIDS Vaccine Program, Frederick, MD 21702 USA. RP Gorelick, RJ (reprint author), SAIC Frederick Inc, Natl Canc Inst, Basic Res Program, AIDS Vaccine Program, Bldg 535,4th Floor, Frederick, MD 21702 USA. EM gorelick@ncifcrf.gov OI Thomas, James/0000-0002-2509-490X FU NCI NIH HHS [N01-CO-12400, N01CO12400] NR 47 TC 56 Z9 56 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2007 VL 81 IS 8 BP 4367 EP 4370 DI 10.1128/JVI.02357-06 PG 4 WC Virology SC Virology GA 157BF UT WOS:000245692900070 PM 17267494 ER PT J AU Makuc, DM Breen, N Meissner, HI Vernon, SW Cohen, A AF Makuc, Diane M. Breen, Nancy Meissner, Helen I. Vernon, Sally W. Cohen, Alan TI Financial barriers to mammography: Who pays out-of-pocket? SO JOURNAL OF WOMENS HEALTH LA English DT Article ID CANCER SCREENING PRACTICES; CERVICAL-CANCER; UNITED-STATES; OLDER WOMEN; BREAST; IMPACT; INSURANCE; ETHNICITY; SERVICES; COVERAGE AB Objective: This study investigates how out-of-pocket payments for mammograms vary according to the characteristics of women and the states where they reside. Methods: We conducted a cross-sectional analysis for women >= 40 years using data from the 2000 National Health Interview Survey (NHIS) Cancer Control Module linked with state characteristics. Descriptive tabulations and logistic regressions were used to examine characteristics associated with out-of-pocket payment for a woman's most recent mammogram for the subset of approximately 7000 women reporting a mammogram within the past 2 years. Results: In 2000, the majority of women who received a mammogram within the past 2 years paid no out-of-pocket costs: 68% among those aged 40 - 64 and 85% among those aged >= 66. Among women aged 40 - 64 with a recent mammogram, characteristics associated with paying out-of-pocket for the last mammogram were white, non-Hispanic race/ethnicity, being uninsured, having non-HMO private coverage, place of residence outside the Northeast, in a non-metropolitan county, and in a state with low HMO penetration. Conclusions: Public insurance and HMO coverage have been especially effective in eliminating financial barriers to mammography, but women 40 - 64 years with public coverage still lag behind their privately insured counterparts in using mammography. Out-of-pocket costs remain a barrier to use for uninsured women. Older women, although less likely than younger women to pay out-of-pocket for mammograms, remain less likely to use mammography than younger women. C1 Ctr Dis Control & Prevent, Natl Ctr Hlth Stat, Off Anal & Epidemiol, Hyattsville, MD 20782 USA. NCI, Hlth Serv, Div Canc Control & Populat Sci, Rockville, MD USA. NCI, Econ Branch, Div Canc Control & Populat Sci, Rockville, MD USA. NCI, Appl Canc Screening Res Branch, Div Canc Control & Populat Sci, Rockville, MD USA. Univ Texas, Sch Publ Hlth, Div Hlth Promot & Behav Sci, Houston, TX USA. RP Makuc, DM (reprint author), Ctr Dis Control & Prevent, Natl Ctr Hlth Stat, Off Anal & Epidemiol, 3311 Toledo Rd,Room 6207, Hyattsville, MD 20782 USA. EM DMakuc@cdc.gov FU NCI NIH HHS [R01CA97263, R01CA76330] NR 32 TC 13 Z9 13 U1 0 U2 1 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1540-9996 J9 J WOMENS HEALTH JI J. Womens Health PD APR PY 2007 VL 16 IS 3 BP 349 EP 360 DI 10.1089/jwh.2006.0072 PG 12 WC Public, Environmental & Occupational Health; Medicine, General & Internal; Obstetrics & Gynecology; Women's Studies SC Public, Environmental & Occupational Health; General & Internal Medicine; Obstetrics & Gynecology; Women's Studies GA 158GB UT WOS:000245778600007 PM 17439380 ER PT J AU Uribarri, J Cai, W Peppa, M Goodman, S Ferrucci, L Striker, G Vlassara, H AF Uribarri, Jaime Cai, Weiiing Peppa, Melpomeni Goodman, Susan Ferrucci, Luigi Striker, Gary Vlassara, Helen TI Circulating glycotoxins and dietary advanced glycation endproducts: Two links to inflammatory response, oxidative stress, and aging SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID RENAL-FAILURE PATIENTS; END-PRODUCTS; GLYCOXIDATION PRODUCTS; DIABETIC COMPLICATIONS; INSULIN-RESISTANCE; PLASMA-GLUCOSE; RISK-FACTOR; PROTEIN; MICE; FOODS AB Background. Oxidative stress (OS) and inflammatory mediators increase with aging. The levels of advanced glycation endproducts (AGEs), prooxidant factors linked to chronic diseases such as diabetes, cardiovascular disease, and renal disease, also increase with aging. AGEs are readily derived from heat-treated foods. We propose that the excess consumption of certain AGEs via the diet enhances OS and inflammatory responses in healthy adults, especially in elderly persons. Methods. We examined 172 young (<45 years old) and older (>60 years old) healthy individuals to determine whether the concentration of specific serum AGEs (N epsilon-carboxymethyl-lysine [CML] or methylglyoxal [MG] derivatives) were higher in older compared to younger persons and whether, independent of age, they correlated with the intake of dietary AGEs, as well as with circulating markers of OS and inflammation. Results. Body weight, body mass index (BMI), and serum AGE, CML, and MG derivatives were higher in older participants, independent of gender. Serum CML correlated with levels of 8-isoprostanes (r = 0.448, p = .0001) as well as with Homeostasis Model Assessment index (HOMA), an index of insulin resistance (r = 0.247, p = .044). The consumption of dietary AGEs, but not of calories, correlated independently with circulating AGEs (CML: r = 0.415, p = .0001 and MG: r = 0.282, p = .002) as well as with high sensitivity C-reactive protein (hsCRP) (r = 0.200, p = .042). Conclusions. Circulating indicators of AGEs (CML and MG derivatives), although elevated in older participants, correlate with indicators of inflammation and OS across all ages. Indicators of both AGEs and OS are directly influenced by the intake of dietary AGEs, independent of age or energy intake. Thus, reduced consumption of these oxidants may prove a safe economic policy to prevent age-related diseases, especially in an aging population. C1 CUNY Mt Sinai Sch Med, Dept Geriatr, Dept Expt Diabet & Aging, New York, NY 10029 USA. CUNY Mt Sinai Sch Med, Dept Med, Div Nephrol, New York, NY 10029 USA. NIA, Longitudinal Studies Sect, Clin Res Branch, NIH, Baltimore, MD 21224 USA. RP Uribarri, J (reprint author), CUNY Mt Sinai Sch Med, Dept Geriatr, Dept Expt Diabet & Aging, Box 1640,1 Gustave Levy Pl, New York, NY 10029 USA. FU Intramural NIH HHS [Z99 AG999999]; NCRR NIH HHS [M01 RR000071, M01-RR-00071]; NIA NIH HHS [AG-09453, AG-23188, R01 AG009453, R37 AG023188] NR 44 TC 199 Z9 208 U1 2 U2 15 PU GERONTOLOGICAL SOC AMER PI WASHINGTON PA 1030 15TH ST NW, STE 250, WASHINGTON, DC 20005202-842 USA SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD APR PY 2007 VL 62 IS 4 BP 427 EP 433 PG 7 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 228WN UT WOS:000250763400014 PM 17452738 ER PT J AU Houston, DK Cesari, M Ferrucci, L Cherubini, A Maggio, D Bartali, B Johnson, MA Schwartz, GG Kritchevskyl, SB AF Houston, Denise K. Cesari, Matteo Ferrucci, Luigi Cherubini, Antonio Maggio, Dario Bartali, Benedetta Johnson, Mary Ann Schwartz, Gary G. Kritchevskyl, Stephen B. TI Association between vitamin D status and physical performance: The InCHIANTI study SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; MUSCLE STRENGTH; SECONDARY HYPERPARATHYROIDISM; D SUPPLEMENTATION; ELDERLY-WOMEN; CALCIUM SUPPLEMENTATION; OLDER-PEOPLE; D DEFICIENCY; BODY SWAY; 25-HYDROXYVITAMIN-D AB Background. Vitamin D status has been hypothesized to play a role in musculoskeletal function. Using data from the InCHIANTI study, we examined the association between vitamin D status and physical performance. Methods. A representative sample of 976 persons aged 65 years or older at study baseline were included. Physical performance was assessed using a short physical performance battery (SPPB) and handgrip strength. Multiple linear regression was used to examine the association between vitamin D (serum 25OHD), parathyroid hormone (PTH), and physical performance adjusting for sociodemographic variables, behavioral characteristics, body mass index, season, cognition, health conditions, creatinine, hemoglobin, and albumin. Results. Approximately 28.8% of women and 13.6% of men had vitamin D levels indicative of deficiency (serum 25OHD < 25.0 nmol/L) and 74.9% of women and 51.0% of men had vitamin D levels indicative of vitamin D insufficiency (serum 25OHD < 50.0 nmol/L). Vitamin D levels were significantly associated with SPPB score in men (beta coefficient [standard error (SE)]: 0.38 [0.18], p = .04) and handgrip strength in men (2.44 [0.84], p = .004) and women (1.33 [0.53], p = .01). Men and women with serum 25OHD < 25.0 nmol/L had significantly lower SPPB scores whereas those with serum 25OHD < 50 nmol/L had significantly lower handgrip strength than those with serum 25OHD >= 25 and >= 50 nmol/L, respectively (p < .05). PTH was significantly associated with handgrip strength only (p = .01). Conclusions. Vitamin D status was inversely associated with poor physical performance. Given the high prevalence of vitamin D deficiency in older populations, additional studies examining the association between vitamin D status and physical function are needed. C1 Wake Forest Univ, Bowman Gray Sch Med, Dept Internal Med, Sect Gerontol & Geriatr Med, Winston Salem, NC 27157 USA. Univ Florida, Gainesville, FL USA. NIA, Baltimore, MD 21224 USA. Univ Perugia, I-06100 Perugia, Italy. Cornell Univ, Ithaca, NY USA. Univ Georgia, Athens, GA 30602 USA. RP Houston, DK (reprint author), Wake Forest Univ, Bowman Gray Sch Med, Dept Internal Med, Sect Gerontol & Geriatr Med, Med Ctr Blvd, Winston Salem, NC 27157 USA. RI Cesari, Matteo/A-4649-2008; OI Cesari, Matteo/0000-0002-0348-3664; Cherubini, Antonio/0000-0003-0261-9897 FU Intramural NIH HHS [Z99 AG999999]; NIA NIH HHS [P30 AG021332, P30-AG-021332-01]; NIMHD NIH HHS [263 MD 821336, 263 MD 916413] NR 38 TC 153 Z9 157 U1 1 U2 9 PU GERONTOLOGICAL SOC AMER PI WASHINGTON PA 1030 15TH ST NW, STE 250, WASHINGTON, DC 20005202-842 USA SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD APR PY 2007 VL 62 IS 4 BP 440 EP 446 PG 7 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 228WN UT WOS:000250763400016 PM 17452740 ER PT J AU Lewis, W Day, BJ Kohler, JJ Hosseini, SH Chan, SSL Green, EC Haase, CP Keebaugh, ES Long, R Ludaway, T Russ, R Steltzer, J Tioleco, N Santoianni, R Copeland, WC AF Lewis, William Day, Brian J. Kohler, James J. Hosseini, Seyed H. Chan, Sherine S. L. Green, Elgin C. Haase, Chad P. Keebaugh, Erin S. Long, Robert Ludaway, Tomika Russ, Rodney Steltzer, Jeffrey Tioleco, Nina Santoianni, Robert Copeland, William C. TI Decreased mtDNA, oxidative stress, cardiomyopathy, and death from transgenic cardiac targeted human mutant polymerase gamma SO LABORATORY INVESTIGATION LA English DT Article DE mitochondria; Pol gamma; oxidative stress; cardiomyopathy; transgenic ID MITOCHONDRIAL-DNA POLYMERASE; PROGRESSIVE EXTERNAL OPHTHALMOPLEGIA; MANGANESE SUPEROXIDE-DISMUTASE; P55 ACCESSORY SUBUNIT; AUTOSOMAL-DOMINANT; DEOXYNUCLEOTIDE CARRIER; DILATED CARDIOMYOPATHY; MULTIPLE DELETIONS; NUCLEAR-DNA; MOUSE HEART AB POLG is the human gene that encodes the catalytic subunit of DNA polymerase gamma (Pol gamma), the replicase for human mitochondrial DNA (mtDNA). A POLG Y955C point mutation causes human chronic progressive external ophthalmoplegia (CPEO), a mitochondrial disease with eye muscle weakness and mtDNA defects. Y955C POLG was targeted transgenically (TG) to the murine heart. Survival was determined in four TG (+/-) lines and wild- type (WT) littermates (-/-). Left ventricle (LV) performance (echocardiography and MRI), heart rate (electrocardiography), mtDNA abundance ( real time PCR), oxidation of mtDNA (8-OHdG), histopathology and electron microscopy defined the phenotype. Cardiac targeted Y955C POLG yielded a molecular signature of CPEO in the heart with cardiomyopathy (CM), mitochondrial oxidative stress, and premature death. Increased LV cavity size and LV mass, bradycardia, decreased mtDNA, increased 8- OHdG, and cardiac histopathological and mitochondrial EM defects supported and defined the phenotype. This study underscores the pathogenetic role of human mutant POLG and its gene product in mtDNA depletion, mitochondrial oxidative stress, and CM as it relates to the genetic defect in CPEO. The transgenic model pathophysiologically links human mutant Pol gamma, mtDNA depletion, and mitochondrial oxidative stress to the mtDNA replication apparatus and to CM. C1 Emory Univ, Sch Med, Dept Pathol, Atlanta, GA USA. Natl Jewish Med Ctr, Denver, CO USA. Natl Inst Environm Hlth Sci, Lab Mol Genet, NIH, Res Triangle Pk, NC USA. Emory Univ, Sch Med, Dept Radiol, Atlanta, GA USA. RP Lewis, W (reprint author), Emory Univ, Sch Med, Dept Pathol, Atlanta, GA USA. EM wlewis@emory.edu FU Intramural NIH HHS; NHLBI NIH HHS [HL072707, R01 HL059798, R01 HL072707]; NIEHS NIH HHS [Z01 ES065078-12, Z01 ES065080-11] NR 50 TC 74 Z9 79 U1 0 U2 4 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD APR PY 2007 VL 87 IS 4 BP 326 EP 335 DI 10.1038/labinvest.3700523 PG 10 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA 148UY UT WOS:000245103200002 PM 17310215 ER PT J AU Linnoila, RI Jensen-Taubman, S Kazanjian, A Grimes, HL AF Linnoila, R. Ilona Jensen-Taubman, Sandra Kazanjian, Avedis Grimes, H. Leighton TI Loss of GFI1 impairs pulmonary neuroendorine cell proliferation, but the neuroendocrine phenotype has limited impact on post-naphthalene airway repair SO LABORATORY INVESTIGATION LA English DT Article DE Gfi1; naphthalene; neuroendocrine; PNEC ID FINGER TRANSCRIPTION FACTOR; NEUROEPITHELIAL BODIES; IL-2-INDEPENDENT GROWTH; TERMINAL BRONCHIOLES; LUNG CARCINOMAS; ENDOCRINE-CELLS; GENE; DIFFERENTIATION; PROTEIN; MICE AB Naphthalene exposure kills lung airway epithelial (Clara) cells, but is rapidly followed by Clara cell reconstitution coincident with proliferation of pulmonary neuroendocrine cells (PNEC). Although a role for mature PNEC in the reconstitution process has been excluded, the reconstituting progenitor cells have been suggested to enter a transient neuroendocrine (NE) differentiation phase before differentiating to Clara cells. Furthermore, these progenitors were suggested to be the target population for transformation to a NE tumor; small cell lung cancer (SCLC). Although the NE phenotype is central to SCLC oncogenesis, the relevance of NE differentiation to post naphthalene reconstitution remains to be determined. The Growth factor independent-1 (Gfi1) transcription factor is expressed in SCLC and is required for the NE differentiation of PNEC. Gfi1(-/-) mice display a 70% reduction in airway cells that express NE markers, and cells that stain for NE markers show weak expression of some markers. Therefore, to determine the relevance of the NE phenotype to post-naphthalene reconstitution, we examined post-naphthalene reconstitution in Gfi1(-/-) mice. Our analyses indicate that the post- naphthalene regeneration process includes both airway epithelial proliferation and apoptosis. Gfi1 deletion lowered both airway epithelial proliferation and apoptosis; however, the post-naphthalene rate of increase in growth and apoptosis was not significantly different between Gfi1(-/-) mice and wild-type littermates. Moreover, the timing and extent of CC10(+) cell regeneration was unaffected by Gfi1 deletion. These data suggest that neither Gfi1 nor the NE phenotype play a dominant role in the regeneration process. However, the few Gfi1(-/-) cells capable of NE differentiation show a significant reduction in post-naphthalene proliferation. The modest proliferation seen in Gfi1(-/-) NE cells is consistent with the previously proposed role for Gfi1 in controlling neuroendocrine cancer growth. C1 NCI, CCR, Expt Pathol Sect, Cell & Canc Biol Branch,NIH, Bethesda, MD USA. Cincinnati Childrens Hosp, Med Ctr, Div Immunobiol, Cincinnati, OH USA. RP Grimes, HL (reprint author), NCI, CCR, Expt Pathol Sect, Cell & Canc Biol Branch,NIH, Bethesda, MD USA. EM lee.grimes@cchmc.org OI Grimes, H. Leighton/0000-0001-8162-6758 FU Intramural NIH HHS; NCI NIH HHS [R01 CA112405, R01 CA112405-03, CA112405] NR 35 TC 9 Z9 9 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD APR PY 2007 VL 87 IS 4 BP 336 EP 344 DI 10.1038/labinvest.3700527 PG 9 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA 148UY UT WOS:000245103200003 PM 17377622 ER PT J AU Schymick, JC Scholz, SW Fung, HC Britton, A Arepalli, S Gibbs, JR Lombardo, F Matarin, M Kasperaviciute, D Hernandez, DG Crews, C Bruijn, L Rothstein, J Mora, G Restagno, G Chio, A Singleton, A Hardy, J Traynor, B AF Schymick, Jennifer C. Scholz, Sonja W. Fung, Hon-Chung Britton, Angela Arepalli, Sampath Gibbs, J. Raphael Lombardo, Federica Matarin, Mar Kasperaviciute, Dalia Hernandez, Dena G. Crews, Cynthia Bruijn, Lucie Rothstein, Jeffrey Mora, Gabriele Restagno, Gabriella Chio, Adriano Singleton, Andrew Hardy, John Traynor, Bryanj TI Genome-wide genotyping in amyotrophic lateral sclerosis and neurologically normal controls: first stage analysis and public release of data SO LANCET NEUROLOGY LA English DT Article ID SINGLE-NUCLEOTIDE POLYMORPHISMS; MOTOR-NEURON DISEASE; PARKINSON-DISEASE; LINKAGE DISEQUILIBRIUM; ASSOCIATION; POPULATION; GENE; MUTATIONS; ALS; PROTEIN AB Background The cause of sporadic ALS is currently unknown. Despite evidence for a role for genetics, no common genetic variants have been unequivocally linked to sporadic ALS. We sought to identify genetic variants associated with an increased or decreased risk for developing ALS in a cohort of American sporadic cases. Methods We undertook a genome-wide association study using publicly available samples from 276 patients with sporadic ALS and 271 neurologically normal controls. 555 352 unique SNPs were assayed in each sample using the Illumina Infinium II HumanHap55O SNP chip. Findings More than 300 million genotypes were produced in 547 participants. These raw genotype data are freely available on the internet and represent the first publicly accessible SNP data for ALS cases. 34 SNPs with a p value less than 0.0001 (two degrees of freedom) were found, although none of these reached significance after Bonferroni correction. Interpretation We generated publicly available genotype data for sporadic ALS patients and controls. No single locus was definitively associated with increased risk of developing disease, although potentially associated candidate SNPs were identified. C1 NIMH, Sect Dev Genet Epidemiol, NIH, Bethesda, MD 20892 USA. NIMH, Neurogenet Lab, NIH, Bethesda, MD 20892 USA. NIMH, Mol Genet Unit, NIH, Bethesda, MD 20892 USA. NIMH, Computat Biol Core, NIH, Bethesda, MD 20892 USA. UCL, Inst Neurol Studies, London, England. Inst Neurol, Dept Neurodegenerat Dis, London WC1N 3BG, England. Inst Neurol, Dept Mol NEurosci, London WC1N 3BG, England. ASO OIRM S Anna, Mol Genet Unit, Turin, Italy. Johns Hopkins Univ, Dept Neurol, Baltimore, MD 21218 USA. Fdn Salvatore Maugeri, Pavia, Italy. Univ Turin, Turin, Italy. Univ Oxford, Dept Physiol Anat & Genet, Oxford, England. RP Traynor, B (reprint author), NIMH, Sect Dev Genet Epidemiol, NIH, Bethesda, MD 20892 USA. EM traynorb@mail.nih.gov RI Singleton, Andrew/C-3010-2009; Kasperaviciute, Dalia/D-1493-2009; Gibbs, J. Raphael/A-3984-2010; Hardy, John/C-2451-2009; Traynor, Bryan/G-5690-2010; rothstein, jeffrey/C-9470-2013; Matarin, Mar/F-1771-2016; OI Matarin, Mar/0000-0002-4717-5735; Chio, Adriano/0000-0001-9579-5341; Scholz, Sonja/0000-0002-6623-0429 FU Intramural NIH HHS; Medical Research Council [G0701075]; Parkinson's UK [G-0907] NR 44 TC 143 Z9 150 U1 0 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1474-4422 J9 LANCET NEUROL JI Lancet Neurol. PD APR PY 2007 VL 6 IS 4 BP 322 EP 328 DI 10.1016/S1474-4422(07)70037-6 PG 7 WC Clinical Neurology SC Neurosciences & Neurology GA 149KE UT WOS:000245145000013 PM 17362836 ER PT J AU Alvarez, RP Johnson, L Grillon, C AF Alvarez, Ruben P. Johnson, Linda Grillon, Christian TI Contextual-specificity of short-delay extinction in humans: Renewal of fear-potentiated startle in a virtual environment SO LEARNING & MEMORY LA English DT Article ID CONDITIONED FEAR; PANIC DISORDER; REINSTATEMENT; MODULATION; AWARENESS; PARADIGM; EXPOSURE; ANXIETY; REFLEX; MEMORY AB A recent fear-potentiated startle study in rodents suggested that extinction was not context dependent when extinction was conducted after a short delay following acquisition, suggesting that extinction can lead to erasure of fear learning in some circumstances. The main objective of this study was to attempt to replicate these findings in humans by examining the context specificity of short-delay extinction in an ABA renewal procedure using virtual reality environments. A second objective was to examine whether renewal, if any, would be influenced by context conditioning. Subjects underwent differential aversive conditioning in virtual context A, which was immediately followed by extinction in virtual context B. Extinction was followed by tests of renewal in context A and B, with the order counterbalanced across subjects. Results showed that extinction was context dependent. Evidence for renewal was established using fear-potentiated startle as well as skin conductance and fear ratings. In addition, although contextual anxiety was greater in the acquisition context than in the extinction context during renewal, as assessed with startle, context conditioning did not influence the renewal effect. These data do not support the view that extinction conducted shortly after acquisition is context independent. Hence, they do not provide evidence that extinction can lead to erasure of a fear memory established via Pavlovian conditioning. C1 NIMH, Mood & Anxiety Disorders Program, NIH, Bethesda, MD 20892 USA. RP Alvarez, RP (reprint author), NIMH, Mood & Anxiety Disorders Program, NIH, Bethesda, MD 20892 USA. EM alvarezr@mail.nih.gov NR 37 TC 58 Z9 59 U1 2 U2 9 PU COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT PI WOODBURY PA 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2924 USA SN 1072-0502 J9 LEARN MEMORY JI Learn. Mem. PD APR PY 2007 VL 14 IS 4 BP 247 EP 253 DI 10.1101/lm.493707 PG 7 WC Neurosciences; Psychology, Experimental SC Neurosciences & Neurology; Psychology GA 162BI UT WOS:000246060900003 PM 17412963 ER PT J AU Xiang, QS Ye, FQ AF Xiang, Qing-San Ye, Frank Q. TI Correction for geometric distortion and N/2 ghosting in EPI by phase labeling for additional coordinate encoding (PLACE) SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article; Proceedings Paper CT 14th Annual Meeting of the International-Society-for-Magnetic-Resonance-in-Medicine CY MAY 06-12, 2006 CL Seattle, WA SP Int Soc Magnet Resonance Med DE EPI artifacts; geometric distortion correction; N/2 ghost correction; phase encoding; k-t space ID REFERENCE-SCAN; ARTIFACT REDUCTION; IMAGING METHODS; ECHO EPI; IMAGES; NMR; MRI AB Echo-planar imaging (EPI) is vulnerable to geometric distortion and N/2 ghosting. These artifacts can be analyzed with an intuitive k-t space tool, and here we propose a simple method for their correction. In a slightly modified additional EPI acquisition, we sample the k-t space with a shift in k(y) by adding a small area to the phase-encoding (PE) gradient. Physically, the added gradient area creates a relative phase ramp across the object and directly encodes the undistorted original y-coordinate of each voxel into a phase difference between two distorted complex images, in a method called "phase labeling for additional coordinate encoding" (PLACE). The phase information is then used to map the mismapped signals back to their original locations for geometric and intensity correction. Smoothing of expanded complex data matrix effectively reduces noise in the differential phase map and allows subpixel warping. The two acquired images can also be averaged to effectively suppress the N/2 ghost. Efficient correction for both artifacts can be achieved with three acquisitions. These acquisitions can also serve as reference scans to correct for geometric distortion and/or N/2 ghost artifacts on all images in a time series. The technique was successfully demonstrated in phantom and animal studies. C1 British Columbia Childrens Hosp, Dept Radiol, Vancouver, BC V6H 3V4, Canada. NIMH, Neurophysiol Imaging Facil, NIH, Bethesda, MD 20892 USA. RP Xiang, QS (reprint author), British Columbia Childrens Hosp, Dept Radiol, 4480 Oak St, Vancouver, BC V6H 3V4, Canada. EM xiang@physics.ubc.ca NR 30 TC 64 Z9 65 U1 1 U2 9 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0740-3194 J9 MAGN RESON MED JI Magn. Reson. Med. PD APR PY 2007 VL 57 IS 4 BP 731 EP 741 DI 10.1002/mrm.21187 PG 11 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 153YS UT WOS:000245474600012 PM 17390358 ER PT J AU Devasahayam, N Subramanian, S Murugesan, R Hyodo, F Matsumoto, KI Mitchell, JB Krishna, MC AF Devasahayam, Nallathamby Subramanian, Sankaran Murugesan, Ramachandran Hyodo, Fuminori Matsumoto, Ken-Ichiro Mitchell, James B. Krishna, Murali C. TI Strategies for improved temporal and spectral resolution in in vivo oximetric imaging using time-domain EPR SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article DE SPI; RF FT EPR; EPR imaging; oximetry; tumor hypoxia ID ELECTRON-PARAMAGNETIC-RESONANCE; CONTINUOUS-WAVE; BREAST-CANCER; BOLD MRI; SPECTROSCOPY; TUMOR; PROBE; RELAXATION; RADICALS; SOLIDS AB A radiofrequency (RF) time-domain electron paramagnetic resonance (EPR) instrument operating at 300, 600, and 750 MHz was used to image tumor hypoxia with high spatial and temporal resolution. A high-speed signal-averaging Peripheral Component Interconnect (PCI) board with flexibility in the input signal level and the number of digitized samples per free induction decay (FID) was incorporated into the receive arm of the spectrometer. This enabled effective and fast averaging of FIDs. Modification of the phase-encoding protocol, and replacement of the General Purpose Interface Bus (GPIB)-based handshake with a PCI-based D/A board for direct control of the gradient amplifier decreased the gradient settling and communication overhead times by nearly two orders of magnitude. Cyclically-ordered phase sequence (CYCLOPS) phase cycling was implemented to correct for pulse imperfections and cancel out unwanted constant signals. These upgrades considerably enhanced the performance of the imager in terms of image collection time, sensitivity, and temporal resolution. We demonstrated this by collecting a large number of 2D images successively and rapidly. The results show that it is feasible to achieve accurate, 2D pO(2) maps of tumor hypoxia with 1-mm(2) resolution and minimal artifacts using a set of multigradient images within an acceptable measuring time of about 3 s, and 3D maps can be obtained in less than 1 min. C1 NCI, Radiat Biol Branch, Ctr Canc Res, Bethesda, MD 20892 USA. Madurai Kamaraj Univ, Sch Chem, Madurai 625021, Tamil Nadu, India. Natl Inst Radiol Sci, Heavy Ion Radiobiol Res Grp, Chiba 260, Japan. RP Krishna, MC (reprint author), NIH, Bldg 10,Room B3 B69, Bethesda, MD 20892 USA. EM murali@helix.nih.gov NR 32 TC 25 Z9 25 U1 0 U2 4 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0740-3194 J9 MAGN RESON MED JI Magn. Reson. Med. PD APR PY 2007 VL 57 IS 4 BP 776 EP 783 DI 10.1002/mrm.21194 PG 8 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 153YS UT WOS:000245474600016 PM 17390350 ER PT J AU Park, JY Javor, ED Cochran, EK DePaoli, AM Gorden, P AF Park, Jean Y. Javor, Edward D. Cochran, Elaine K. DePaoli, Alex M. Gorden, Phillip TI Long-term efficacy of leptin replacement in patients with Dunnigan-type familial partial lipodystrophy SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article ID REVERSES INSULIN-RESISTANCE; OBESE GENE; LAMIN A/C; CONGENITAL LIPODYSTROPHY; RECOMBINANT LEPTIN; MISSENSE MUTATIONS; HIV LIPODYSTROPHY; DIABETES-MELLITUS; HEPATIC STEATOSIS; R482Q MUTATION AB The Dunnigan-type familial partial lipodystrophy (FPLD) is characterized by a variable loss of fat from the extremities and trunk and excess subcutaneous fat in the chin and supraclavicular area. Associated metabolic abnormalities include hypoleptinemia, insulin resistance, and dyslipidemia. Our goal was to observe changes in metabolic parameters for patients with FPLD on long-term leptin replacement and to compare the metabolic characteristics seen in FPLD with those seen in generalized lipodystrophy (GL) from our previous studies. This was an open-label study of 6 patients with FPLD receiving maximal doses of oral antidiabetic and lipid-lowering medications at baseline. Recombinant leptin was given through twice-daily subcutaneous injections at a maximal dose of 0.08 mg/kg per day over 12 months to simulate normal to high normal physiologic levels. Triglycerides were reduced by 65% at 4 months (749 +/- 331 to 260 +/- 58 mg/dL) and significantly reduced at 12 months for 5 patients (433 +/- 125 to 247 +/- 69 mg/dL; P = .03). Total cholesterol also decreased (280 +/- 49 to 231 +/- 41 mg/dL; P = .01). Insulin sensitivity and fasting glucose levels (190 +/- 26 to 151 +/- 15 mg/dL; P < .01) improved. Glucose tolerance and glycosylated hemoglobin levels (8.4% +/- 0.6% to 8.0% +/- 0.4%; P = .07) did not change. As shown in patients with GL, patients with FPLD have improvement in triglycerides, fasting glucose, and insulin sensitivity with leptin replacement. In contrast to the patients with GL, the patients with FPLD are older, have higher leptin levels, and notably lower insulin secretion for a similar degree of hyperglycemia. Low-dose recombinant methionyl human leptin for patients with FPLD has an important role in improving triglycerides, beyond that of available lipid-lowering agents. In improving glycemic control, normalization of glucose tolerance in hypoinsulinemic patients with FPLD requires insulin and leptin therapy. This is the first study to examine the effects of long-term leptin replacement in patients with FPLD. (c) 2007 Elsevier Inc. All rights reserved. C1 NIDDKD, Clin Endocrinol Branch, NIH, Bethesda, MD 20892 USA. Amgen Inc, Thousand Oaks, CA 91320 USA. RP Gorden, P (reprint author), NIDDKD, Clin Endocrinol Branch, NIH, Bethesda, MD 20892 USA. EM phillipg@intra.niddk.nih.gov FU Intramural NIH HHS [Z01 DK047052-01] NR 36 TC 49 Z9 53 U1 2 U2 4 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0026-0495 J9 METABOLISM JI Metab.-Clin. Exp. PD APR PY 2007 VL 56 IS 4 BP 508 EP 516 DI 10.1016/j.metabol.2006.11.010 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 153CJ UT WOS:000245409000013 PM 17379009 ER PT J AU Henderson, MPA Billen, LP Kim, PK Andrews, DW AF Henderson, Matthew P. A. Billen, Lieven P. Kim, Peter K. Andrews, David W. TI Cell-free analysis of tail-anchor protein targeting to membranes SO METHODS LA English DT Article DE tail-anchor proteins; post-translational; topology; targeting; specificity; protein dependence; endoplasmic reticulum; mitochondria; liposomes; membrane biogenesis ID MITOCHONDRIAL OUTER-MEMBRANE; ENDOPLASMIC-RETICULUM MEMBRANE; INTRACELLULAR MEMBRANES; CYTOCHROME-C; SIGNAL; INSERTION; TRANSLOCATION; APOPTOSIS; BCL-2; BAX AB We describe cell-free strategies for analysis of the biogenesis of membrane proteins. Special emphasis is placed on the unique challenges presented by tail-anchor proteins for the analysis of. targeting specificity, binding to membranes such as endoplasmic reticulum, mitochondria and pure lipid membranes, protein topology in the membrane and the dependence of various steps on additional proteins. Tail-anchor proteins such as cytochrome b(5), Bcl-2 and Bcl-X-L are used to address the suitability of both cell-free techniques based on in vitro transcription translation systems and the use of purified recombinant protein and pure lipid membranes. Together these cell-free systems permit detailed characterization of membrane protein biogenesis. (c) 2006 Elsevier Inc. All rights reserved. C1 McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada. NIH, Cell Biol & Metab Branch, Bethesda, MD 20892 USA. RP Andrews, DW (reprint author), McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada. EM Andrewsd@mcmaster.ca OI Andrews, David/0000-0002-9266-7157 NR 44 TC 10 Z9 10 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1046-2023 J9 METHODS JI Methods PD APR PY 2007 VL 41 IS 4 BP 427 EP 438 DI 10.1016/j.ymeth.2006.07.004 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 157YJ UT WOS:000245757200009 PM 17367715 ER PT J AU Jacobs, M Togbe, D Fremond, C Samarina, A Allie, N Botha, T Carlos, D Parida, SK Grivennikov, S Nedospasov, S Monteiro, A Le Bert, M Quesniaux, V Ryffel, B AF Jacobs, Muazzam Togbe, Dieudonnee Fremond, Cecile Samarina, Arina Allie, Nasiema Botha, Tania Carlos, Daniela Parida, Shreemanta K. Grivennikov, Sergei Nedospasov, Sergei Monteiro, Analbery Le Bert, Marc Quesniaux, Valerie Ryffel, Bemhard TI Tumor necrosis factor is critical to control tuberculosis infection SO MICROBES AND INFECTION LA English DT Review DE tumor necrosis factor; tuberculosis; reactivation of infection; gene deficient mice ID INTRACELLULAR BACTERIAL-INFECTION; NITRIC-OXIDE SYNTHASE; BOVIS BCG INFECTION; TNF-DEFICIENT MICE; MYCOBACTERIUM-TUBERCULOSIS; LATENT TUBERCULOSIS; GRANULOMA-FORMATION; FACTOR-ALPHA; T-CELLS; FACTOR RECEPTOR AB Tumor necrosis factor (TNF) is critical and non-redundant to control Mycobacterium tuberculosis infection and cannot be replaced by other proinflarnmatory cytokines. Overproduction of TNF may cause immunopathology, while TNF neutralization reactivates latent and chronic, controlled infection, which is relevant for the use of neutralizing TNF therapies in patients with rheumatoid arthritis. (c) 2007 Elsevier Masson SAS. All rights reserved. C1 CNRS, UMR 6218, Inst Transgenose, F-45071 Orleans, France. Univ Cape Town, Inst Infect Dis & Mol Med, ZA-7925 Cape Town, South Africa. NCI, Canc Res Ctr, Mol Immunoregulat Lab, Ft Detrick, MD 21702 USA. Cape Peninsula Univ Technol, Cape Town, South Africa. Russian Acad Sci, Engelhardt Inst Mol Biol, Lab Mol Immunol, Moscow 119991, Russia. Max Planck Inst, Berlin, Germany. Univ USP, Ribeirao Preto, Brazil. RP Ryffel, B (reprint author), CNRS, UMR 6218, Inst Transgenose, 3B Rue Ferollerie, F-45071 Orleans, France. EM bryffel@cnrs-orleans.fr RI Nedospasov, Sergei/J-5936-2013; Nedospasov, Sergei/L-1990-2015; Nedospasov, Sergei/Q-7319-2016; OI Le Bert, Marc/0000-0002-5945-3800; Parida, Shreemanta K/0000-0003-2129-6691; Brodovcky, Tania/0000-0003-2364-2623 NR 35 TC 53 Z9 57 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1286-4579 J9 MICROBES INFECT JI Microbes Infect. PD APR PY 2007 VL 9 IS 5 BP 623 EP 628 DI 10.1016/j.micinf.2007.02.002 PG 6 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 176TK UT WOS:000247107600010 PM 17409008 ER PT J AU Meijsing, SH Elbi, C Luecke, HF Hager, GL Yamamoto, KR AF Meijsing, Sebastiaan H. Elbi, Cern Luecke, Hans F. Hager, Gordon L. Yamamoto, Keith R. TI The ligand binding domain controls glucocorticoid receptor dynamics independent of ligand release SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID LIVING CELLS; DEXAMETHASONE 21-MESYLATE; MOLECULAR CHAPERONES; CHROMATIN-STRUCTURE; AMINO-ACID; IN-VIVO; PROTEIN; TRANSCRIPTION; AFFINITY; P23 AB Ligand binding to the glucocorticoid receptor (GR) results in receptor binding to glucocorticoid response elements (GREs) and the formation of transcriptional regulatory complexes. Equally important, these complexes are continuously disassembled, with active processes driving GR off GREs. We found that cochaperone p23-dependent disruption of GR-driven transcription depended on the ligand binding domain (LBD). Next, we examined the importance of the LBD and of ligand dissociation in GR-GRE dissociation in living cells. We showed in fluorescence recovery after photobleaching studies that dissociation of GR from GREs is faster in the absence of the LBD. Furthermore, GR interaction with a target promoter revealed ligand-specific exchange rates. However, using covalently binding ligands, we demonstrated that ligand dissociation is not required for receptor dissociation from GREs. Overall, these studies showed that activities impinging on the LBD regulate GR exchange with GREs but that the dissociation of GR from GREs is independent from ligand dissociation. C1 Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94107 USA. NIH, Lab Receptor Biol & Gene Express, Bethesda, MD 20892 USA. RP Yamamoto, KR (reprint author), Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, 600 16th St,Room GH-S574, San Francisco, CA 94107 USA. EM yamamoto@cmp.ucsf.edu RI Luecke, Hartmut "Hudel"/F-4712-2012 OI Luecke, Hartmut "Hudel"/0000-0002-4938-0775 FU Intramural NIH HHS NR 42 TC 30 Z9 32 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2007 VL 27 IS 7 BP 2442 EP 2451 DI 10.1128/MCB.01570-06 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 153CY UT WOS:000245410500003 PM 17261597 ER PT J AU Menendez, D Inga, A Snipe, J Krysiak, O Schonfelder, G Resnick, MA AF Menendez, Daniel Inga, Alberto Snipe, Joyce Krysiak, Oliver Schoenfelder, Gilbert Resnick, Michael A. TI A single-nucleotide polymorphism in a half-binding site creates p53 and estrogen receptor control of vascular endothelial growth factor receptor 1 SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID ENDOTHELIAL GROWTH-FACTOR; TUMOR-SUPPRESSOR P53; TRANSCRIPTION-FACTOR; HUMAN GENOME; RESPONSE ELEMENTS; VEGF RECEPTORS; FLT-1; ANGIOGENESIS; EXPRESSION; SEQUENCE AB Interactions between master regulatory pathways provide higher-order controls for cellular regulation. Recently, we reported a C -> T single-nucleotide polymorphism (SNP) in the vascular endothelial growth factor receptor 1 (VEGFR-1/Flt1) promoter that merges human VEGF and p53 pathways. This finding suggested a new layer in environmental controls of a pathway relevant to several diseases. The Flt1-T SNP created what appeared to be a half-site p53 target response element (RE). The absence of information about p53 gene responsiveness mediated by half-site REs led us to address how it influences Flt1 expression. We now identify a second regulatory sequence comprising a partial RE for estrogen receptors (ERs) upstream of the p53 binding site. Surprisingly, this provides for synergistic stimulation of transcription specifically at the Flt1-T allele through the combined action of ligand-bound ER and stress-induced p53. In addition to demonstrating direct control of FR1 expression by ER and p53 proteins acting as sequence-specific transcription factors at half-site REs, we establish a new interaction between three master regulatory pathways, p53, ER, and VEGF. The mechanism of joint regulation through half-sites is likely relevant to transcriptional control of other targets and expands the number of genes that may be directly controlled in master regulatory networks. C1 NIEHS, Mol Genet Lab, NIH, Res Triangle Pk, NC 27709 USA. Natl Inst Canc Res, IST, Mol Mutgagenesis Unit, Genoa, Italy. Charite Univ Med Berlin, Inst Clin Pharmacol Toxicol, Berlin, Germany. RP Resnick, MA (reprint author), NIEHS, Mol Genet Lab, NIH, MD3-01,111 Alexander Dr, Res Triangle Pk, NC 27709 USA. EM resnick@niehs.nih.gov FU Intramural NIH HHS NR 60 TC 40 Z9 42 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2007 VL 27 IS 7 BP 2590 EP 2600 DI 10.1128/MCB.01742-06 PG 11 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 153CY UT WOS:000245410500016 PM 17242190 ER PT J AU Fitzpatrick, GV Pugacheva, EM Shin, JY Abdullaev, Z Yang, YW Khatod, K Lobanenkov, VV Higgins, MJ AF Fitzpatrick, Galina V. Pugacheva, Elena M. Shin, Jong-Yeon Abdullaev, Ziedulla Yang, Youwen Khatod, Kavita Lobanenkov, Victor V. Higgins, Michael J. TI Allele-specific binding of CTCF to the multipartite imprinting control region KvDMR1 SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID REPRESSIVE HISTONE METHYLATION; BECKWITH-WIEDEMANN-SYNDROME; ENHANCER-BLOCKING ACTIVITY; INSULATOR PROTEIN CTCF; CHROMATIN INSULATOR; DNA METHYLATION; H19/IGF2 LOCUS; ANTISENSE RNA; FREE DOMAINS; GENE AB Paternal deletion of the imprinting control region (ICR) KvDMR1 results in loss of expression of the Kcnq1ot1 noncoding RNA and derepression of flanking paternally silenced genes. Truncation of Kcnq1ot1 also results in the loss of imprinted expression of these genes in most cases, demonstrating a role for the RNA or its transcription in gene silencing. However, enhancer-blocking studies indicate that KvDMR1 also contains chromatin insulator or silencer activity. In this report we demonstrate by electrophoretic mobility shift assays and chromatin immunoprecipitation the existence of two CTCF binding sites within KvDMR1 that are occupied in vivo only on the unmethylated paternally derived allele. Methylation interference and mutagenesis allowed the precise mapping of protein-DNA contact sites for CTCF within KvDMR1. Using a luciferase reporter assay, we mapped the putative transcriptional promoter for Kcnq1ot1 upstream and to a site functionally separable from enhancer-blocking activity and CTCF binding sites. Luciferase reporter assays also suggest the presence of an additional cis-acting element in KvDMR1 upstream of the putative promoter that can function as an enhancer. These results suggest that the KvDMR1 ICR consists of multiple, independent cis-acting modules. Dissection of KvDMR1 into its functional components should help elucidate the mechanism of its function in vivo. C1 Roswell Pk Canc Inst, Dept Canc Genet, Buffalo, NY 14263 USA. NIAID, NIH, Lab Immunopathol, Bethesda, MD 20892 USA. RP Lobanenkov, VV (reprint author), Roswell Pk Canc Inst, Dept Canc Genet, Elm & Carlton St, Buffalo, NY 14263 USA. EM vlobanenkov@niad.nih.gov; michael.higgins@roswellpark.org OI Lobanenkov, Victor/0000-0001-6665-3635 FU Intramural NIH HHS; NCI NIH HHS [CA 16056, CA 89426, R01 CA089426, P30 CA016056] NR 51 TC 60 Z9 63 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2007 VL 27 IS 7 BP 2636 EP 2647 DI 10.1128/MCB.02036-06 PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 153CY UT WOS:000245410500020 PM 17242189 ER PT J AU Kim, A Zhao, H Ifrim, I Dean, A AF Kim, AeRi Zhao, Hui Ifrim, Ina Dean, Ann TI beta-globin intergenic transcription and histone acetylation dependent on an enhancer SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID LOCUS-CONTROL REGION; RNA-POLYMERASE-II; HYPERSENSITIVE SITE 2; CREB-BINDING PROTEIN; CHROMATIN DOMAIN; GENE-EXPRESSION; IN-VIVO; HEMATOPOIETIC TRANSCRIPTION; LYSINE METHYLATION; HS2 ENHANCER AB Histone acetyltransferases are associated with the elongating RNA polymerase II (Pol II) complex, supporting the idea that histone acetylation and transcription are intertwined mechanistically in gene coding sequences. Here, we studied the establishment and function of histone acetylation and transcription in noncoding sequences by using a model locus linking the beta-globin HS2 enhancer and the embryonic epsilon-globin gene in chromatin. An intact HS2 enhancer that recruits RNA Pol H is required for intergenic transcription and histone H3 acetylation and K4 methylation between the enhancer and target gene. RNA Pol H recruitment to the target gene TATA box is not required for the intergenic transcription or intergenic histone modifications, strongly implying that they are properties conferred by the enhancer. However, Pol 11 recruitment at HS2, intergenic transcription, and intergenic histone modification are not sufficient for transcription or modification of the target gene: these changes require initiation at the TATA box of the gene. The results suggest that intergenic and genic transcription complexes are independent and possibly differ from one another. C1 NIDDK, Lab Cellular & Dev Biol, NIH, Bethesda, MD 20892 USA. Pusan Natl Univ, Dept Mol Biol, Coll Nat Resources, Pusan 609735, South Korea. RP Dean, A (reprint author), NIDDK, Lab Cellular & Dev Biol, NIH, Bethesda, MD 20892 USA. EM anndean@helix.nih.gov FU Intramural NIH HHS NR 49 TC 29 Z9 29 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2007 VL 27 IS 8 BP 2980 EP 2986 DI 10.1128/MCB.02337-06 PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 155DP UT WOS:000245558300017 PM 17283048 ER PT J AU Daw, MI Scott, HL Isaac, JTR AF Daw, Michael I. Scott, Helen L. Isaac, John T. R. TI Developmental synaptic plasticity at the thalamocortical input to barrel cortex: Mechanisms and roles SO MOLECULAR AND CELLULAR NEUROSCIENCE LA English DT Review DE glutamate; LTP; LTD; AMPA receptor; NMDA receptor; kainate receptor; critical period ID LONG-TERM POTENTIATION; EXPERIENCE-DEPENDENT PLASTICITY; PROTEIN-KINASE-A; MOUSE SOMATOSENSORY CORTEX; DEVELOPING VISUAL-CORTEX; OCULAR DOMINANCE PLASTICITY; AMPA RECEPTOR TRAFFICKING; NR2A SUBUNIT EXPRESSION; KAINATE RECEPTORS; SILENT SYNAPSES AB The thalamocortical (TC) input to layer IV provides the major pathway for ascending sensory information to the mammalian sensory cortex. During development there is a dramatic refinement of this input that underlies the maturation of the topographical map in layer IV. Over the last 10 years our understanding of the mechanisms of the developmental and experience-driven changes in synaptic function at TC synapses has been greatly advanced. Here we describe these studies that point to a key role for NMDA receptor-dependent synaptic plasticity, a role for kainate receptors and for a rapid maturation in GABAergic inhibition. The expression mechanisms of some of the forms of neonatal synaptic plasticity are novel and, in combination with other mechanisms, produce a layer IV circuit that exhibits functional properties necessary for mature sensory processing. (c) 2007 Elsevier Inc. All rights reserved. C1 Univ Bristol, Dept Anat, MRC Ctr Synapt Plast, Bristol BS8 1TD, Avon, England. NINDS, NIH, Bethesda, MD 20892 USA. RP Daw, MI (reprint author), Univ Bristol, Dept Anat, MRC Ctr Synapt Plast, Univ Walk, Bristol BS8 1TD, Avon, England. EM dawmicha@mail.nih.gov RI Scott, Helen/A-6307-2009; OI Scott, Helen/0000-0003-2656-4034; Daw, Michael/0000-0002-8374-5977 FU Intramural NIH HHS [001-1789-088, Z99 NS999999]; Wellcome Trust NR 131 TC 45 Z9 46 U1 3 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1044-7431 J9 MOL CELL NEUROSCI JI Mol. Cell. Neurosci. PD APR PY 2007 VL 34 IS 4 BP 493 EP 502 DI 10.1016/j.mcn.2007.01.001 PG 10 WC Neurosciences SC Neurosciences & Neurology GA 156NK UT WOS:000245656000001 PM 17329121 ER PT J AU Grigoryev, DN Ma, SF Shimoda, LA Johns, RA Lee, B Garcia, JGN AF Grigoryev, Dmitry N. Ma, Shwu-Fan Shimoda, Larissa A. Johns, Roger A. Lee, Byungkook Garcia, Joe G. N. TI Exon-based mapping of microarray probes: Recovering differential gene expression signal in underpowered hypoxia experiment SO MOLECULAR AND CELLULAR PROBES LA English DT Article DE gene expression profiling; microarray; oligonucleotide probe; mouse model; hypoxia; pulmonary hypertension ID TISSUE FACTOR; SEQUENCE; DUPLICATION; TOOL AB There is an immense collection of underpowered Affymetrix gene array experiments. Although a majority of these experiments generated biologically feasible results, the considerable fraction of assays failed to identify expected transcriptional changes. There is an unused potential of Affymetrix probe-set redundancy for common exonic and UTR regions. We hypothesized that group analysis of multiple probe-sets which hybridize to the same exon or UTR will increase array discriminating power of transcriptional changes. To test this hypothesis, we analyzed Affymetrix mouse probe-sets that share the same exon using blocking feature of the Significance Analysis of Microarrays (SAM). Two-thousand two-hundred one exon-sharing probe-sets targeting 1011 transcripts were identified by mapping 36701 MG-U74v2 probe-sets to genomic alignments of 3,971,086 known mouse transcripts. Using the blocking feature of SAM with an underpowered (two microarrays per experimental condition) mouse hypoxia-induced pulmonary hypertension model, we identified 24 genes that were significantly (FDR < 5%) affected by hypoxia but were not detected by regular SAM. The relevance of the four newly identified genes (Mig6, F3, Bmp6, and Ndrg1) to known hypoxia-associated responses was confirmed by PubMatrix; and hypoxia-induced up-regulation of Mig6 expression was validated by real-time RT-PCR. We demonstrated that analysis of exon-sharing probe-sets allowed discovery of additional hypoxia-affected genes in an underpowered array experiment. This method will facilitate re-evaluation of existing underpowered Affymetrix gene expression profiles. (c) 2006 Elsevier Ltd. All rights reserved. C1 Johns Hopkins Univ, Sch Med, Baltimore, MD USA. Univ Chicago, Pritzker Sch Med, Chicago, IL 60637 USA. NCI, Mol Modeling & Bioinformat Sect, NIH, Bethesda, MD 20892 USA. RP Grigoryev, DN (reprint author), Johns Hopkins Univ, Sch Med, Baltimore, MD USA. EM dgrigor1@jhmi.edu; sma@medicine.bsd.uchicago.edu; shimodal@welch.jhu.edu; rajohns@jhmi.edu; bk@nih.gov; jgarcia@medicine.bsd.uchicago.edu RI Garcia, Joe/E-8862-2010 FU NHLBI NIH HHS [F32 HL074590, F32 HL74590-01A1, HL-69340, P50 HL073994] NR 23 TC 6 Z9 6 U1 0 U2 0 PU ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0890-8508 J9 MOL CELL PROBE JI Mol. Cell. Probes PD APR PY 2007 VL 21 IS 2 BP 134 EP 139 DI 10.1016/j.mcp.2006.09.002 PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Cell Biology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Cell Biology GA 135VX UT WOS:000244183100008 PM 17071053 ER PT J AU Rogozin, IB Wolf, YI Carmel, L Koonin, EV AF Rogozin, Igor B. Wolf, Yuri I. Carmel, Liran Koonin, Eugene V. TI Ecdysozoan clade rejected by genome-wide analysis of rare amino acid replacements SO MOLECULAR BIOLOGY AND EVOLUTION LA English DT Article DE phylogenetic analysis; cladistics; rare genomic changes; coelomata; ecdysozoa; microsporidia ID LONG-BRANCH ATTRACTION; RIBOSOMAL-RNA SEQUENCES; ANIMAL PHYLOGENY; ENCEPHALITOZOON-CUNICULI; MOLECULAR PHYLOGENY; MAXIMUM-LIKELIHOOD; EUKARYOTIC GENOMES; MODEL ORGANISMS; GENE-SEQUENCES; HIGH-FREQUENCY AB As the number of sequenced genomes from diverse walks of life rapidly increases, phylogenetic analysis is entering a new era: reconstruction of the evolutionary history of organisms on the basis of full-scale comparison of their genomes. In addition to brute force, genome-wide analysis of alignments, rare genomic changes (RGCs) that are thought to comprise derived shared characters of individual clades are increasingly used in genome-wide phylogenetic studies. We propose a new type of RGCs designated RGC_CAMs (after Conserved Amino acids-Multiple substitutions), which are inferred using a genome-scale analysis of protein and underlying nucleotide sequence alignments. The RGC_CAM approach utilizes amino acid residues conserved in major eukaryotic lineages, with the exception of a few species comprising a putative clade, and selects for phylogenetic inference only those amino acid replacements that require 2 or 3 nucleotide substitutions, in order to reduce homoplasy. The RGC_CAM analysis was combined with a procedure for rigorous statistical testing of competing phylogenetic hypotheses. The RGC_CAM method is shown to be robust to branch length differences and taxon sampling. When applied to animal phylogeny, the RGC_CAM approach strongly supports the coelomate clade that unites chordates with arthropods as opposed to the ecdysozoan (molting animals) clade. This conclusion runs against the view of animal evolution that is currently prevailing in the evo-devo community. The final solution to the coelomate-ecdysozoa controversy will require a much larger set of complete genome sequences representing diverse animal taxa. It is expected that RGC_CAM and other RGC-based methods will be crucial for these future, definitive phylogenetic studies. C1 Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. RP Rogozin, IB (reprint author), Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. EM koonin@ncbi.nlm.nih.gov RI Carmel, Liran/A-9681-2008 FU Intramural NIH HHS NR 86 TC 38 Z9 42 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0737-4038 J9 MOL BIOL EVOL JI Mol. Biol. Evol. PD APR PY 2007 VL 24 IS 4 BP 1080 EP 1090 DI 10.1093/molbev/msm029 PG 11 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA 152HS UT WOS:000245353200022 PM 17299026 ER PT J AU Gattegno, T Mittal, A Valansi, C Nguyen, KCQ Hall, DH Chernomordik, LV Podbilewicz, B AF Gattegno, Tamar Mittal, Aditya Valansi, Clari Nguyen, Ken C. Q. Hall, David H. Chernomordik, Leonid V. Podbilewicz, Benjamin TI Genetic control of fusion pore expansion in the epidermis of Caenorhabditis elegans SO MOLECULAR BIOLOGY OF THE CELL LA English DT Article ID SEMLIKI-FOREST-VIRUS; CELL-CELL FUSION; INDUCED MEMBRANE-FUSION; MALE TAIL TIP; C-ELEGANS; MYOBLAST FUSION; ENVELOPE GLYCOPROTEIN; TEMPERATURE-DEPENDENCE; BIOLOGICAL-MEMBRANES; PROTEIN AB Developmental cell fusion is found in germlines, muscles, bones, placentae, and stem cells. In Caenorhabditis elegans 300 somatic cells fuse during development. Although there is extensive information on the early intermediates of viralinduced and intracellular membrane fusion, little is known about late stages in membrane fusion. To dissect the pathway of cell fusion in C. elegans embryos, we use genetic and kinetic analyses using live-confocal and electron microscopy. We simultaneously monitor the rates of multiple cell fusions in developing embryos and find kinetically distinct stages of initiation and completion of membrane fusion in the epidermis. The stages of cell fusion are differentially blocked or retarded in eff-1 and idf-1 mutants. We generate kinetic cell fusion maps for embryos grown at different temperatures. Different sides of the same cell differ in their fusogenicity: the left and right membrane domains are fusion-incompetent, whereas the anterior and posterior membrane domains fuse with autonomous kinetics in embryos. All but one cell pair can initiate the formation of the largest syncytium. The first cell fusion does not trigger a wave of orderly fusions in either direction. Ultrastructural studies show that epidermal syncytiogenesis require eff-1 activities to initiate and expand membrane merger. C1 Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel. NICHHD, Sect Membrane Biol, Lab Cellular & Mol Biophys, NIH, Bethesda, MD 20892 USA. Albert Einstein Coll Med, Ctr C Elegans Anat, Dept Neurosci, Bronx, NY 10461 USA. RP Podbilewicz, B (reprint author), Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel. EM podbilew@tx.technion.ac.il RI Mittal, Aditya/E-3087-2010; Podbilewicz, Benjamin/N-4825-2014; OI Mittal, Aditya/0000-0002-4030-0951; Podbilewicz, Benjamin/0000-0002-0411-4182 FU Intramural NIH HHS; NCRR NIH HHS [R24 RR012596]; NIH HHS [R24 OD010943] NR 89 TC 17 Z9 21 U1 0 U2 3 PU AMER SOC CELL BIOLOGY PI BETHESDA PA 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA SN 1059-1524 J9 MOL BIOL CELL JI Mol. Biol. Cell PD APR PY 2007 VL 18 IS 4 BP 1153 EP 1166 DI 10.1091/mbc.E06-09-0855 PG 14 WC Cell Biology SC Cell Biology GA 153NV UT WOS:000245443100003 PM 17229888 ER PT J AU Aoyagi, S Archer, TK AF Aoyagi, Sayura Archer, Trevor K. TI Dynamic histone acetylation/deacetylation with progesterone receptor-mediated transcription SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID TUMOR VIRUS PROMOTER; BREAST-CANCER CELLS; IN-VIVO; DEPENDENT TRANSCRIPTION; DEACETYLASE INHIBITORS; MMTV TRANSCRIPTION; GENE-EXPRESSION; CHROMATIN; ACETYLATION; NUCLEOSOME AB Histone acetylation is a highly dynamic posttranslational modification that plays an important role in gene expression. Previous work showed that promoter histone deacetylation is accompanied by progesterone receptor (PR)-mediated activation of the mouse mammary tumor virus (MMTV) promoter. We investigated the role of this deacetylation and found that this histone deacetylation is not a singular event. In fact, histone acetylation at the MMTV promoter is highly dynamic, with an initial increase in acetylation followed by an eventual net deacetylation of histone H4. The timing of increase in acetylation of H4 coincides with the time at which PR, RNA polymerase II, and histone acetyltransferases cAMP response element-binding protein (CREB)-binding protein and p300 are recruited to the MMTV promoter. The timing in which histone H4 deacetylation occurs (after PR and RNA polymerase II recruitment) and the limited effect that trichostatin A and small interfering RNA knockdown of histone deacetylase (HDAC)3 have on MMTV transcription suggests that this deacetylation activity is not required for the initiation of PR-mediated transcription. Interestingly, two HDACs, HDAC1 and HDAC3, are already present at the MMTV before transcription activation. HDAC association at the MMTV promoter fluctuates during the hormone treatment. In particular, HDAC3 is temporarily undetected at the MMTV promoter within minutes after hormone treatment when the histone H4 acetylation increases but returns to the promoter near the time when histone acetylation levels start to decline. These results demonstrate the dynamic nature of coactivator/corepressor-promoter association and histone modifications such as acetylation during a transcription activation event. C1 Natl Inst Environm Hlth Sci, Chromatin & Gene Express Sect, Lab Mol Carcinogenesis, NIH, Res Triangle Pk, NC 27709 USA. RP Archer, TK (reprint author), Natl Inst Environm Hlth Sci, Chromatin & Gene Express Sect, Lab Mol Carcinogenesis, NIH, 111 Alexander Dr,POB 12233,MD D4-01, Res Triangle Pk, NC 27709 USA. EM archer1@niehs.nih.gov FU Intramural NIH HHS NR 68 TC 27 Z9 27 U1 0 U2 2 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD APR PY 2007 VL 21 IS 4 BP 843 EP 856 DI 10.1210/me.2006-0244 PG 14 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 150OW UT WOS:000245227100005 PM 17227884 ER PT J AU Fyfe, JC Kurzhals, RL Hawkins, MG Wang, P Yuhki, N Giger, U Van Winkle, TJ Haskins, ME Patterson, DF Henthorn, PS AF Fyfe, John C. Kurzhals, Rebeccah L. Hawkins, Michelle G. Wang, Ping Yuhki, Naoya Giger, Urs Van Winkle, Thomas J. Haskins, Mark E. Patterson, Donald F. Henthorn, Paula S. TI A complex rearrangement in GBE1 causes both perinatal hypoglycemic collapse and late-juvenile-onset neuromuscular degeneration in glycogen storage disease type IV of Norwegian forest cats SO MOLECULAR GENETICS AND METABOLISM LA English DT Article DE glycogenosis; glycogen storage; branching enzyme; enzymopathy; neuromuscular degeneration; hypoglycemia; genomic rearrangement ID BRANCHING ENZYME DEFICIENCY; HUMAN GENETIC-DISEASE; REPLICATION SLIPPAGE; WEB SERVER; MODEL; PREDICTION; MUTATIONS; INSERTION; MYOPATHY; BLAST AB Deficiency of glycogen branching enzyme (GBE) activity causes glycogen storage disease type IV (GSD IV), an autosomal recessive error of metabolism. Abnormal glycogen accumulates in myocytes, hepatocytes, and neurons, causing variably progressive, benign to lethal organ dysfunctions. A naturally occurring orthologue of human GSD IV was described previously in Norwegian forest cats (NFC). Here, we report that while most affected kittens die at or soon after birth, presumably due to hypoglycemia, survivors of the perinatal period appear clinically normal until onset of progressive neuromuscular degeneration at 5 months of age. Molecular investigation of affected cats revealed abnormally spliced GBE1 mRNA products and lack of GBE cross-reactive material in liver and muscle. Affected cats are homozygous for a complex rearrangement of genomic DNA in GBE1, constituted by a 334 bp insertion at the site of a 6.2kb deletion that extends from intron 11 to intron 12 (g.IVS11+1552(-)IVS12-1339 de16.2kb ins334 bp), removing exon 12. An allele-specific, PCR-based test demonstrates that the rearrangement segregates with the disease in the GSD IV kindred and is not found in unrelated normal cats. Screening of 402 privately owned NFC revealed 58 carriers and 4 affected cats. The molecular characterization of feline GSD IV will enhance further studies of GSD IV pathophysiology and development of novel therapies in this unique animal model. (c) 2006 Elsevier Inc. All rights reserved. C1 Michigan State Univ, Coll Vet Med, Lab Comparat Med Genet, E Lansing, MI 48824 USA. Univ Penn, Sch Vet Med, Med Genet Sect, Philadelphia, PA 19104 USA. NCI, Lab Genom Divers, Frederick, MD 21702 USA. Univ Penn, Sch Vet Med, Dept Pathobiol, Philadelphia, PA 19104 USA. RP Fyfe, JC (reprint author), Michigan State Univ, Coll Vet Med, Lab Comparat Med Genet, E Lansing, MI 48824 USA. EM fyfe@cvm.msu.edu FU NCRR NIH HHS [RR02512, P40 RR002512] NR 42 TC 10 Z9 11 U1 0 U2 9 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1096-7192 J9 MOL GENET METAB JI Mol. Genet. Metab. PD APR PY 2007 VL 90 IS 4 BP 383 EP 392 DI 10.1016/j.ymgme.2006.12.003 PG 10 WC Endocrinology & Metabolism; Genetics & Heredity; Medicine, Research & Experimental SC Endocrinology & Metabolism; Genetics & Heredity; Research & Experimental Medicine GA 162IC UT WOS:000246079200006 PM 17257876 ER PT J AU Xiao, Z Ray, M Jiang, CC Clark, AB Rogozin, IB Diaz, M AF Xiao, Zheng Ray, Madhumita Jiang, Chuancang Clark, Alan B. Rogozin, Igor B. Diaz, Marilyn TI Known components of the immunoglobulin A : T mutational machinery are intact in Burkitt lymphoma cell lines with G : C bias SO MOLECULAR IMMUNOLOGY LA English DT Article DE somatic hypermutation; AID; immunoglobulin; class switch recombination; mismatch repair; B cells; Burkitt lymphoma; translesion synthesis ID INDUCED CYTIDINE DEAMINASE; DNA-POLYMERASE-ETA; CLASS-SWITCH RECOMBINATION; SINGLE-STRANDED-DNA; SOMATIC HYPERMUTATION OCCURS; HEAVY-CHAIN LOCUS; IG HYPERMUTATION; ANTIBODY DIVERSIFICATION; DEFICIENT MICE; CUTTING EDGE AB The basis for mutations at A:T base pairs in immunoglobulin hypermutation and defining how AID interacts with the DNA of the immunoglobulin locus are major aspects of the immunoglobulin mutator mechanism where questions remain unanswered. Here, we examined the pattern of mutations generated in mice deficient in various DNA repair proteins implicated in A:T mutation and found a previously unappreciated bias at G:C base pairs in spectra from mice simultaneously deficient in DNA mismatch repair and uracil DNA glycosylase. This suggests a strand-biased DNA transaction for AID delivery which is then masked by the mechanism that introduces A:T mutations. Additionally, we asked if any of the known components of the A:T mutation machinery underscore the basis for the paucity of A:T mutations in the Burkitt lymphoma cell lines, Ramos and BL2. Ramos and BL2 cells were proficient in MSH2/MSH6-mediated mismatch repair, and express high levels of wild-type, full-length DNA polymerase eta. In addition, Ramos cells have high levels of uracil DNA glycosylase protein and are proficient in base excision repair. These results suggest that Burkitt lymphoma cell lines may be deficient in an unidentified factor that recruits the machinery necessary for A:T mutation or that AID-mediated cytosine deamination in these cells may be processed by conventional base excision repair truncating somatic hypermutation at the G:C phase. Either scenario suggests that cytosine deamination by AID is not enough to trigger A:T mutation, and that additional unidentified factors are required for full spectrum hypermutation in vivo. Published by Elsevier Ltd. C1 Natl Inst Environm Hlth Sci, Genet Mol Lab, NIH, Res Triangle Pk, NC 27709 USA. Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. RP Diaz, M (reprint author), Natl Inst Environm Hlth Sci, Genet Mol Lab, NIH, D3-01,111 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM diaz@niehs.nih.gov FU Intramural NIH HHS; NIEHS NIH HHS [Z01 ES101603-03] NR 75 TC 20 Z9 20 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0161-5890 J9 MOL IMMUNOL JI Mol. Immunol. PD APR PY 2007 VL 44 IS 10 BP 2659 EP 2666 DI 10.1016/j.molimm.2006.12.006 PG 8 WC Biochemistry & Molecular Biology; Immunology SC Biochemistry & Molecular Biology; Immunology GA 156WS UT WOS:000245681200018 PM 17240451 ER PT J AU Alrefai, RH Winter, DB Bohr, VA Gearhart, PJ AF Alrefai, Rudaina H. Winter, David B. Bohr, Vilhelm A. Gearhart, Patricia J. TI Nucleotide excision repair in an immunoglobulin variable gene is less efficient than in a housekeeping gene SO MOLECULAR IMMUNOLOGY LA English DT Article DE nucleotide excision repair; transcription; immunoglobulin gene; dihydrofolate reductase gene ID COMPLEMENTATION GROUP-C; DNA-REPAIR; XERODERMA-PIGMENTOSUM; SOMATIC HYPERMUTATION; SWITCH RECOMBINATION; HISTONE ACETYLATION; PYRIMIDINE DIMERS; MICE DEFICIENT; ACTIVE GENE; B-CELLS AB Immunoglobulin variable genes undergo several unusual genetic modifications to generate diversity, such as gene rearrangement, gene conversion, somatic hypermutation, and heavy chain class switch recombination. In view of these specialized processes, we examined the possibility that variable genes have intrinsic characteristics that allow them to be processed differently in the course of basic DNA transactions as well. This hypothesis was studied in an experimental system to gauge the relative efficiency of a DNA repair pathway, nucleotide excision repair, on a variable gene and a housekeeping gene. DNA damage was induced by ultraviolet light in murine hybridoma B cells, and repair was measured over time by an alkaline Southern blot technique, which detected removal of cyclobutane pyrimidine dimers. The rate of DNA repair in a rearranged variable gene, V(H)S107, was compared to that in the dihydrofolate reductase gene. Although both genes were actively transcribed, the V(H)S107 gene was repaired less efficiently than the dihydrofolate reductase gene. These results suggest that variable genes have inherent properties that affect the efficiency of nucleotide excision repair. (c) 2007 Elsevier Ltd. All rights reserved. C1 NIA, Lab Mol Gerontol, NIH, Baltimore, MD 21224 USA. RP Gearhart, PJ (reprint author), NIA, Lab Mol Gerontol, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA. EM gearhartp@grc.nia.nih.gov FU Intramural NIH HHS; NIA NIH HHS [Z01 AG000732-10] NR 25 TC 2 Z9 2 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0161-5890 J9 MOL IMMUNOL JI Mol. Immunol. PD APR PY 2007 VL 44 IS 11 BP 2800 EP 2805 DI 10.1016/j.molimm.2007.01.018 PG 6 WC Biochemistry & Molecular Biology; Immunology SC Biochemistry & Molecular Biology; Immunology GA 170MH UT WOS:000246667000002 PM 17336386 ER PT J AU Koonin, EV AF Koonin, Eugene V. TI Chance and necessity in cellular response to challenge SO MOLECULAR SYSTEMS BIOLOGY LA English DT Editorial Material ID ESCHERICHIA-COLI; EVOLUTION; EXPRESSION; DYNAMICS; NETWORK C1 NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20892 USA. RP Koonin, EV (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bldg 10, Bethesda, MD 20892 USA. NR 13 TC 4 Z9 4 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 1744-4292 J9 MOL SYST BIOL JI Mol. Syst. Biol. PD APR PY 2007 VL 3 AR 107 DI 10.1038/msb4100152 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 164BN UT WOS:000246207700011 PM 17453048 ER PT J AU Kritz, AB Nicol, CG Dishart, KL Nelson, R Holbeck, S Von Seggern, DJ Work, LM Mcvey, JH Nicklin, SA Baker, AH AF Kritz, Angelika B. Nicol, Campbell G. Dishart, Kate L. Nelson, Ruth Holbeck, Susan Von Seggern, Dan J. Work, Lorraine M. McVey, John H. Nicklin, Stuart A. Baker, Andrew H. TI Adenovirus 5 fibers mutated at the putative HSPG-binding site show restricted retargeting with targeting peptides in the HI loop SO MOLECULAR THERAPY LA English DT Article ID HEPARAN-SULFATE GLYCOSAMINOGLYCANS; MEDIATED GENE DELIVERY; CAR-BINDING; CELL INFECTION; RECEPTOR; TROPISM; INTEGRIN; VECTORS; LIVER; TRANSDUCTION AB Adenoviral vectors are commonly used for liver-directed gene therapy following systemic administration owing to their strong propensity for hepatocyte transduction. However, many disease applications would benefit from the delivery of adenoviruses to alternate tissues via this route. Research has thus focused on stripping the virus of native hepatic tropism in conjunction with modifying virus capsid proteins to incorporate novel tropism. Recently, the KO1S* adenovirus serotype 5 fiber mutant, devoid of both coxsackie and adenovirus receptor binding in the fiber knob domain and mutated at the putative heparan sulphate proteoglycan binding site in the fiber shaft, was shown to possess strikingly poor hepatic tropism in mice, rats, and non-human primates. Thus, it is an ideal candidate for retargeting strategies. We therefore assessed the ability of peptide-modified KO1S* fibers to retarget adenovirus. Peptide insertions were well tolerated and virions produced to high titers. However, expected retargeting at the level of transduction was not observed, despite cell-binding studies showing enhanced vector targeting at the cell surface. Cy3 labeling studies showed retarded trafficking of S*-containing fibers. Taken together, our data demonstrates that KO1S* mutant fibers are ineffective for cell retargeting strategies. C1 Univ Glasgow, Div Cardiovasc & Med Sci, BHF Glasgow Cardiovasc Res Ctr, Glasgow G12 8TA, Lanark, Scotland. NCI, Dev Therapeut Program, Bethesda, MD 20892 USA. Scripps Res Inst, Dept Immunol, La Jolla, CA 92037 USA. Univ London Imperial Coll Sci & Technol, MRC, Ctr Clin Sci, Dept Haemostasis & Thrombosis, London, England. RP Baker, AH (reprint author), Univ Glasgow, Div Cardiovasc & Med Sci, BHF Glasgow Cardiovasc Res Ctr, 126 Univ Pl, Glasgow G12 8TA, Lanark, Scotland. EM ab11f@clinmed.gla.ac.uk FU Biotechnology and Biological Sciences Research Council [BB/E02145X/1]; Medical Research Council [G0400052] NR 33 TC 38 Z9 39 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 1525-0016 J9 MOL THER JI Mol. Ther. PD APR PY 2007 VL 15 IS 4 BP 741 EP 749 DI 10.1038/mt.sj.6300094 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA 150KJ UT WOS:000245215400014 PM 17245351 ER PT J AU Ackerman, MJ AF Ackerman, Michael J. TI Next generation networking: distributed multimedia information for healthcare SO MULTIMEDIA TOOLS AND APPLICATIONS LA English DT Article; Proceedings Paper CT 9th International Conference on Distributed Multimedia Systems (DMS 03)/6th International Conference on Visual Information Systems (VIS 2003) CY SEP 24, 2003-SEP 26, 2006 CL Miami, FL DE quality of service; next generation networking; distributed multimedia; healthcare; scalable information infrastructure; Internet2; abilene AB This paper is derived from a keynote address given that the DMS-03 meeting. It chronicles the need and development of next generation networks (NGN) in the United States. Specific organizational examples are derived from the Internet2-Abilene Network. The technical characteristics of a next generation network versus the Internet are discussed. Examples are given from the point of view of the need for a quality of service based network to deliver distributed multimedia healthcare information to the point of need. The concepts of network trust and of a network based scalable information infrastructure for the reliable delivery of distributed multimedia information is also introduced. C1 Natl Lib Med, Bethesda, MD 20894 USA. RP Ackerman, MJ (reprint author), Natl Lib Med, Bethesda, MD 20894 USA. EM ackerman@nlm.nih.gov NR 1 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-7501 J9 MULTIMED TOOLS APPL JI Multimed. Tools Appl. PD APR PY 2007 VL 33 IS 1 BP 5 EP 11 DI 10.1007/s11042-006-0093-4 PG 7 WC Computer Science, Information Systems; Computer Science, Software Engineering; Computer Science, Theory & Methods; Engineering, Electrical & Electronic SC Computer Science; Engineering GA 149FZ UT WOS:000245133800002 ER PT J AU Di Rezze, S Gupta, S Durastanti, V Millefiorini, E Pozzilli, C Bagnato, F AF Di Rezze, S. Gupta, S. Durastanti, V. Millefiorini, E. Pozzilli, C. Bagnato, F. TI An exploratory study on interferon beta dose effect in reducing size of enhancing lesions in multiple sclerosis SO MULTIPLE SCLEROSIS LA English DT Article DE contrast-enhancing lesions; inflammation; interferon beta; lesion size; magnetic resonance imaging; multiple sclerosis ID SPIN-ECHO; MS; MRI; PATHOLOGY; TERM AB Sixty-two patients with multiple sclerosis (MS) were imaged monthly over a six-month (ie, seven monthly magnetic resonance images [MRI]) natural history period (NHP). Thereafter, patients were randomized to receive 11 or 33 mu g of subcutaneously injected interferon beta 1a (IFN beta-1a) with imaging monthly for nine months and at months 12, 18 and 24 of therapy phase (TP). In the present exploratory post hoc analysis, the authors evaluated IFN beta-1 a dose effect on reducing the size of contrast-enhancing lesions (CELs). MRIs performed at months 0, 3 and 6 of NHP and at months 3, 6, 9, 18 and 24 of TP were analysed. While a significant reduction in mean number of CELs was observed in both treatment groups of patients, the mean total volume and size of CELs was reduced only in patients undergoing therapy with 33 mu g of IFN beta-1a. The latter suggests a significant dose effect exerted by IFN beta-1 a in the evolution of CELs' dimensions during therapy. C1 Univ Roma La Sapienza, Dept Neurol Sci, Rome, Italy. New York Med Coll, Valhalla, NY 10595 USA. NINDS, Neuroimmunol Branch, NIH, Bethesda, MD 20892 USA. RP Bagnato, F (reprint author), Bldg 10,Room 5B16,9000 Rockville Pike, Bethesda, MD 20892 USA. EM bagnatof@ninds.nih.gov OI millefiorini, enrico/0000-0001-5318-3849 NR 18 TC 4 Z9 4 U1 0 U2 0 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1352-4585 J9 MULT SCLER JI Mult. Scler. PD APR PY 2007 VL 13 IS 3 BP 343 EP 347 DI 10.1177/1352458506071172 PG 5 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA 161LL UT WOS:000246016300004 PM 17439903 ER PT J AU Yewdell, JW Lev, A AF Yewdell, Jonathan W. Lev, Avital TI Self-reporting peptides illuminate the MHC grove SO NATURE CHEMICAL BIOLOGY LA English DT Editorial Material ID CELLS; BINDING C1 NIAID, Viral Dis Lab, NIH, Bethesda, MD 20892 USA. RP Yewdell, JW (reprint author), NIAID, Viral Dis Lab, NIH, Bldg 33,33 N Dr, Bethesda, MD 20892 USA. EM jyewdell@nih.gov RI yewdell, jyewdell@nih.gov/A-1702-2012 NR 4 TC 0 Z9 0 U1 0 U2 2 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1552-4450 J9 NAT CHEM BIOL JI Nat. Chem. Biol. PD APR PY 2007 VL 3 IS 4 BP 201 EP 202 DI 10.1038/nchembio0407-201 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 148UW UT WOS:000245103000004 PM 17372601 ER PT J AU Armstrong, AY Calis, KA Nelson, LM AF Armstrong, Alicia Y. Calis, Karim A. Nelson, Lawrence M. TI Do survivors of childhood cancer have an increased incidence of primary ovarian insufficiency? SO NATURE CLINICAL PRACTICE ENDOCRINOLOGY & METABOLISM LA English DT Editorial Material DE alkylating agent; childhood cancer; Hodgkin's lymphoma; premature menopause; radiation therapy ID MENOPAUSE C1 NICHHD, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA. NICHHD, Reprod Biol & Med Branch, NIH, Bethesda, MD 20892 USA. NICHHD, Drug Informat Serv, NIH, Bethesda, MD 20892 USA. RP Nelson, LM (reprint author), NICHHD, Dev Endocrinol Branch, NIH, 10 Ctr Dr,Room 1-3330, Bethesda, MD 20892 USA. EM lawrence_nelson@nih.gov NR 6 TC 4 Z9 4 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 1745-8366 J9 NAT CLIN PRACT ENDOC JI Nat. Clin. Pract. Endocrinol. Metab. PD APR PY 2007 VL 3 IS 4 BP 326 EP 327 DI 10.1038/ncpendmet0452 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 148UQ UT WOS:000245102400003 PM 17290234 ER PT J AU Pan-Hammarstrom, Q Salzer, U Du, L Bjorkander, J Cunningham-Rundles, C Nelson, DL Bacchelli, C Gaspar, HB Offer, S Behrens, TW Grimbacher, B Hammarstrom, L AF Pan-Hammarstrom, Qiang Salzer, Ulrich Du, Likun Bjorkander, Janne Cunningham-Rundles, Charlotte Nelson, David L. Bacchelli, Chiara Gaspar, H. Bobby Offer, Steven Behrens, Timothy W. Grimbacher, Bodo Hammarstrom, Lennart TI Reexamining the role of TACI coding variants in common variable immunodeficiency and selective IgA deficiency SO NATURE GENETICS LA English DT Letter C1 Gothenburg Univ, Sahlgrenska Hosp, S-41345 Gothenburg, Sweden. Mt Sinai Sch Med, Div Clin Immunol, New York, NY 10029 USA. NCI, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. Inst Child Hlth, London WC1N 1EH, England. Univ Minnesota, Ctr Immunol, Sch Med, Minneapolis, MN 55455 USA. UCL, Dept Immunol, Royal Free Hosp, London 3NW 2QG, England. RP Pan-Hammarstrom, Q (reprint author), Gothenburg Univ, Sahlgrenska Hosp, S-41345 Gothenburg, Sweden. EM qiang.pan-hammarstrom@ki.se; lennart.hammarstrom@ki.se RI Offer, Steven/A-7461-2013 FU Medical Research Council [G0501468]; NIAID NIH HHS [P01 AI061093-039001, P01 AI061093-020002, P01 AI061093-050002, P01 AI061093-06, P01 AI061093-03, P01 AI061093-029001, P01 AI061093-065490, P01 AI061093-02, P01 AI061093-010002, P01 AI061093-059001, P01 AI061093-05, P01 AI061093-040002, P01 AI061093-019001, P01 AI061093-065495, P01 AI061093-049001, P01 AI061093-04, P01 AI061093-030002, P01 AI061093]; PHS HHS [N01-A1-30070] NR 7 TC 119 Z9 124 U1 0 U2 5 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD APR PY 2007 VL 39 IS 4 BP 429 EP 430 DI 10.1038/ng0407-429 PG 2 WC Genetics & Heredity SC Genetics & Heredity GA 151EE UT WOS:000245271200005 PM 17392797 ER PT J AU Scheper, GC van der Klok, T van Andel, RJ van Berkel, CGM Sissler, M Smet, J Muravina, TI Serkov, SV Uziel, G Bugiani, M Schiffmann, R Krageloh-Mann, I Smeitink, JAM Florentz, C Van Coster, R Pronk, JC van der Knaap, MS AF Scheper, Gert C. van der Klok, Thom van Andel, Rob J. van Berkel, Carola G. M. Sissler, Marie Smet, Joel Muravina, Tatjana I. Serkov, Sergey V. Uziel, Graziella Bugiani, Marianna Schiffmann, Raphael Kraegeloh-Mann, Ingeborg Smeitink, Jan A. M. Florentz, Catherine Van Coster, Rudy Pronk, Jan C. van der Knaap, Marjo S. TI Mitochondrial aspartyl-tRNA synthetase deficiency causes leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation SO NATURE GENETICS LA English DT Article ID VANISHING WHITE-MATTER; DISEASE; FIBROBLASTS; TRANSLATION; MUTATIONS; DEFECTS; GENE AB Leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation (LBSL) has recently been defined based on a highly characteristic constellation of abnormalities observed by magnetic resonance imaging and spectroscopy(1). LBSL is an autosomal recessive disease, most often manifesting in early childhood. Affected individuals develop slowly progressive cerebellar ataxia, spasticity and dorsal column dysfunction, sometimes with a mild cognitive deficit or decline. We performed linkage mapping with microsatellite markers in LBSL families and found a candidate region on chromosome 1, which we narrowed by means of shared haplotypes. Sequencing of genes in this candidate region uncovered mutations in DARS2, which encodes mitochondrial aspartyl-tRNA synthetase, in affected individuals from all 30 families. Enzyme activities of mutant proteins were decreased. We were surprised to find that activities of mitochondrial complexes from fibroblasts and lymphoblasts derived from affected individuals were normal, as determined by different assays. C1 Vrije Univ Amsterdam, Dept Pediat & Child Neurol, NL-1081 HV Amsterdam, Netherlands. Univ Strasbourg 1, CNRS, Inst Biol Mol & Cellulaire, F-67084 Strasbourg, France. Univ Hosp, Dept Pediat, Div Neurol & Metab, B-9000 Ghent, Belgium. Ist Nazl Neurol, Child Neurol Dept, I-20133 Milan, Italy. Russian Acad Med Sci, Burdenko Neurosurg Inst, Dept Neuroimaging, Moscow, Russia. NINDS, Dev & Metab Neurol BRanch, NIH, Bethesda, MD 20892 USA. Univ Tubingen, Childrens Hosp, Dept Pediat Neurol, D-72076 Tubingen, Germany. Radboud Univ Nijmegen, Nijmegen Ctr Mitochondrial Disorders, Ctr Med, Dept Pediat, NL-6500 HB Nijmegen, Netherlands. Vrije Univ Amsterdam, Ctr Med, Dept Human Genet, NL-1081 BT Amsterdam, Netherlands. RP Scheper, GC (reprint author), Vrije Univ Amsterdam, Dept Pediat & Child Neurol, De Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands. EM gc.scheper@vumc.nl RI Smeitink, Jan/D-6064-2011; Smeitink, Jan/C-1351-2013; Scheper, Gert/D-7001-2014 NR 30 TC 227 Z9 236 U1 0 U2 4 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD APR PY 2007 VL 39 IS 4 BP 534 EP 539 DI 10.1038/ng2013 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA 151EE UT WOS:000245271200025 PM 17384640 ER PT J AU Deng, L Langley, RJ Brown, PH Xu, G Teng, L Wang, Q Gonzales, MI Callender, GG Nishimura, MI Topalian, SL Mariuzza, RA AF Deng, Lu Langley, Ries J. Brown, Patrick H. Xu, Gang Teng, Leslie Wang, Qian Gonzales, Monica I. Callender, Glenda G. Nishimura, Michael I. Topalian, Suzanne L. Mariuzza, Roy A. TI Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor SO NATURE IMMUNOLOGY LA English DT Article ID LIGAND-SPECIFIC OLIGOMERIZATION; ANTIGEN RECOGNITION; PEPTIDE-MHC; ANALYTICAL ULTRACENTRIFUGATION; CRYSTAL-STRUCTURE; COMPLEX; AUTOIMMUNITY; BINDING; PROTEIN; ACTIVATION AB Structural studies of complexes of T cell receptor ( TCR) and peptide-major histocompatibility complex ( MHC) have focused on TCRs specific for foreign antigens or native self. An unexplored category of TCRs includes those specific for self determinants bearing alterations resulting from disease, notably cancer. We determined here the structure of a human melanoma-specific TCR ( E8) bound to the MHC molecule HLA-DR1 and an epitope from mutant triosephosphate isomerase. The structure had features intermediate between 'anti-foreign' and autoimmune TCR-peptide-MHC class II complexes that may reflect the hybrid nature of altered self. E8 manifested very low affinity for mutant triosephosphate isomerase-HLA-DR1 despite the highly tumor-reactive properties of E8 cells. A second TCR ( G4) had even lower affinity but underwent peptide-specific formation of dimers, suggesting this as a mechanism for enhancing low-affinity TCR-peptide-MHC interactions for T cell activation. C1 NCI, Canc Res Ctr, NIH, Surg Branch, Bethesda, MD 20892 USA. Univ Maryland, Ctr Adv Res Biotechnol, WM Keck Lab Struct Biol, Inst Biotechnol, Rockville, MD 20850 USA. Univ Chicago, Dept Surg, Chicago, IL 60637 USA. NIH, Div Bioengn & Phys Sci, Off Res Serv, Bethesda, MD 20892 USA. Med Univ S Carolina, Hollings Canc Ctr, Charleston, SC 29425 USA. Johns Hopkins Univ, Sch Med, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21231 USA. RP Topalian, SL (reprint author), Univ Auckland, Dept Mol Med & Pathol, Fac Med & Hlth Sci, Auckland 1, New Zealand. EM stopali1@jhmi.edu; mariuzza@carb.nist.gov OI Langley, Ries/0000-0001-8606-2197 FU Intramural NIH HHS; NIAID NIH HHS [AI036900, R37 AI036900] NR 53 TC 44 Z9 45 U1 0 U2 3 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 1529-2908 J9 NAT IMMUNOL JI Nat. Immunol. PD APR PY 2007 VL 8 IS 4 BP 398 EP 408 DI 10.1038/ni1447 PG 11 WC Immunology SC Immunology GA 148LK UT WOS:000245075900015 PM 17334368 ER PT J AU Uhl, G AF Uhl, George TI Premature poking: impulsivity, cocaine and dopamine SO NATURE MEDICINE LA English DT Editorial Material ID DRUG-ABUSE; VULNERABILITY; POPULATIONS; DEPENDENCE; HUMANS AB 'Impulsivity' occurs frequently in people with addiction and other common disorders such as attention deficit hyperactivity disorder (ADHD). Experiments in rats suggest that reduced dopamine receptor availability in the brain's ventral striatum may underlie links between impulsivity and addiction. C1 NIDA, Mol Neurobiol Branch, US Natl Inst Hlth Intramural Res Program, Baltimore, MD 21224 USA. RP Uhl, G (reprint author), NIDA, Mol Neurobiol Branch, US Natl Inst Hlth Intramural Res Program, POB 5180, Baltimore, MD 21224 USA. EM GUHL@intra.nida.nih.gov NR 12 TC 5 Z9 5 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 1078-8956 J9 NAT MED JI Nat. Med. PD APR PY 2007 VL 13 IS 4 BP 413 EP 414 DI 10.1038/nm0407-413 PG 3 WC Biochemistry & Molecular Biology; Cell Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Cell Biology; Research & Experimental Medicine GA 154YG UT WOS:000245543900018 PM 17415375 ER PT J AU Daw, MI Ashby, MC Isaac, JTR AF Daw, Michael I. Ashby, Michael C. Isaac, John T. R. TI Coordinated developmental recruitment of latent fast spiking interneurons in layer IV barrel cortex SO NATURE NEUROSCIENCE LA English DT Article ID RAT SOMATOSENSORY CORTEX; THALAMOCORTICAL FEEDFORWARD INHIBITION; CRITICAL PERIOD; SYNAPTIC PLASTICITY; EXCITATORY ACTIONS; TRANSGENIC MICE; SILENT SYNAPSES; NEURONS; NEOCORTEX; GABA AB Feedforward inhibitory GABAergic transmission is critical for mature cortical circuit function; in the neonate, however, GABA is depolarizing and believed to have a different role. Here we show that the GABA(A) receptor-mediated conductance is depolarizing in excitatory (stellate) cells in neonatal (postnatal day [P]3-5) layer IV barrel cortex, but GABAergic transmission at this age is not engaged by thalamocortical input in the feedforward circuit and has no detectable circuit function. However, recruitment occurs at P6-7 as a result of coordinated increases in thalamic drive to fast-spiking interneurons, fast-spiking interneuron-stellate cell connectivity and hyperpolarization of the GABA(A) receptor-mediated response. Thus, GABAergic circuits are not engaged by thalamocortical input in the neonate, but are poised for a remarkably coordinated development of feedforward inhibition at the end of the first postnatal week, which has profound effects on circuit function at this critical time in development. C1 Univ Bristol, Dept Anat, MRC, Ctr Synap Plast, Bristol BS8 1TD, Avon, England. NINDS, NIH, Bethesda, MD 20892 USA. RP Isaac, JTR (reprint author), Univ Bristol, Dept Anat, MRC, Ctr Synap Plast, Bristol BS8 1TD, Avon, England. EM isaacj@ninds.nih.gov OI Daw, Michael/0000-0002-8374-5977 FU Intramural NIH HHS; Wellcome Trust NR 47 TC 86 Z9 87 U1 3 U2 5 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA SN 1097-6256 J9 NAT NEUROSCI JI Nat. Neurosci. PD APR PY 2007 VL 10 IS 4 BP 453 EP 461 DI 10.1038/nn1866 PG 9 WC Neurosciences SC Neurosciences & Neurology GA 150PL UT WOS:000245228600016 PM 17351636 ER PT J AU Matsuoka, M Jeang, KT AF Matsuoka, Masao Jeang, Kuan-Teh TI Human T-cell leukaemia virus type 1 (HTLV-1) infectivity and cellular transformation SO NATURE REVIEWS CANCER LA English DT Review ID NF-KAPPA-B; I TAX ONCOPROTEIN; ANAPHASE-PROMOTING COMPLEX; TUMOR-SUPPRESSOR PROTEIN; ADHESION MOLECULE-1 GENE; 1ST HUMAN RETROVIRUS; LYMPHOTROPIC-VIRUS; TRANSCRIPTIONAL ACTIVITY; CENTROSOME AMPLIFICATION; SPINDLE CHECKPOINT AB It has been 30 years since a 'new' leukaemia termed adult T-cell leukaemia (ATL) was described in Japan, and more than 25 years since the isolation of the retrovirus, human T-cell leukaemia virus type 1 (HTLV-1), that causes this disease. We discuss HTLV-1 infectivity and how the HTLV-1 Tax oncoprotein initiates transformation by creating a cellular environment favouring aneuploidy and clastogenic DNA damage. We also explore the contribution of a newly discovered protein and RNA on the HTLV-1 minus strand, HTLV-1 basic leucine zipper factor (HBZ), to the maintenance of virus-induced leukaemia. C1 NIAID, Lab Mol Microbiol, NIH, Bethesda, MD 20892 USA. Kyoto Univ, Lab Virus Immunol, Inst Virus Res, Kyoto, Japan. RP Jeang, KT (reprint author), NIAID, Lab Mol Microbiol, NIH, 4 Ctr Dr, Bethesda, MD 20892 USA. EM kj7e@nih.gov RI Jeang, Kuan-Teh/A-2424-2008; OI Matsuoka, Masao/0000-0002-0473-754X NR 148 TC 402 Z9 414 U1 8 U2 44 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1474-175X J9 NAT REV CANCER JI Nat. Rev. Cancer PD APR PY 2007 VL 7 IS 4 BP 270 EP 280 DI 10.1038/nrc2111 PG 11 WC Oncology SC Oncology GA 149TP UT WOS:000245170000013 PM 17384582 ER PT J AU Subbarao, K Joseph, T AF Subbarao, Kanta Joseph, Tomy TI Scientific barriers to developing vaccines against avian influenza viruses SO NATURE REVIEWS IMMUNOLOGY LA English DT Review ID A H5N1 VIRUS; TO-HUMAN TRANSMISSION; 1918 PANDEMIC VIRUS; REVERSE-GENETICS; MF59-ADJUVANTED INFLUENZA; HONG-KONG; RECEPTOR SPECIFICITY; BALB/C MICE; A/DUCK/SINGAPORE/97 VACCINE; MONOCLONAL-ANTIBODY AB The increasing number of reports of direct transmission of avian influenza viruses to humans underscores the need for control strategies to prevent an influenza pandemic. Vaccination is the key strategy to prevent severe illness and death from pandemic influenza. Despite long-term experience with vaccines against human influenza viruses, researchers face several additional challenges in developing human vaccines against avian influenza viruses. In this Review, we discuss the features of avian influenza viruses, the gaps in our understanding of infections caused by these viruses in humans and of the immune response to them that distinguishes them from human influenza viruses, and the current status of vaccine development. C1 NIAID, Lab Infect Dis, NIH, Bethesda, MD 20892 USA. RP Subbarao, K (reprint author), NIAID, Lab Infect Dis, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM ksubbarao@niaid.nih.gov FU Intramural NIH HHS NR 129 TC 147 Z9 157 U1 4 U2 20 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1474-1733 J9 NAT REV IMMUNOL JI Nat. Rev. Immunol. PD APR PY 2007 VL 7 IS 4 BP 267 EP 278 DI 10.1038/nri2054 PG 12 WC Immunology SC Immunology GA 160VY UT WOS:000245971700013 PM 17363960 ER PT J AU Pollack, IF Jakacki, RI Blaney, SM Hancock, ML Kieran, MW Phillips, P Kun, LE Friedman, H Packer, R Banerjee, A Geyer, JR Goldman, S Poussaint, TY Krasin, MJ Wang, YF Hayes, M Murgo, A Weiner, S Boyett, JM AF Pollack, Ian F. Jakacki, Regina I. Blaney, Susan M. Hancock, Michael L. Kieran, Mark W. Phillips, Peter Kun, Larry E. Friedman, Henry Packer, Roger Banerjee, Anu Geyer, J. Russell Goldman, Stewart Poussaint, Tina Young Krasin, Matthew J. Wang, Yanfeng Hayes, Michael Murgo, Anthony Weiner, Susan Boyett, James M. TI Phase I trial of imatinib in children with newly diagnosed brainstem and recurrent malignant gliomas: A Pediatric Brain Tumor Consortium report SO NEURO-ONCOLOGY LA English DT Article DE brainstem; children; dose-limiting toxicity; enzyme-inducing anticonvulsant drugs; imatinib mesylate; intratumoral hemorrhage; malignant glioma; maximum tolerated dose; phase I trial ID CHRONIC MYELOID-LEUKEMIA; GROWTH-FACTOR RECEPTOR; ABL TYROSINE KINASE; CANCER GROUP; FACTOR PDGF; PHILADELPHIA-CHROMOSOME; MESYLATE STI571; CELL-LINES; INHIBITION; EXPRESSION AB This study estimated the maximum tolerated dose (MTD) of imatinib with irradiation in children with newly diagnosed brainstem gliomas, and those with recurrent malignant intracranial gliomas, stratified according to use of enzyme-inducing anticonvulsant drugs (EIACDs). In the brainstem glioma stratum, imatinib was initially administered twice daily during irradiation, but because of possible association with intratumoral hemorrhage (ITH) was subsequently started two weeks after irradiation. The protocol was also amended to exclude children with prior hemorrhage. Twenty-four evaluable patients received therapy before the amendment, and three of six with a brainstem tumor experienced dose-limiting toxicity (DLT): one had asymptomatic ITH, one had grade 4 neutropenia and, one had renal insufficiency. None of 18 patients with recurrent glioma experienced DLT. After protocol amendment, 3 of 16 patients with brainstem glioma and 2 of 11 patients with recurrent glioma who were not receiving EIACDs experienced ITH DLTs, with three patients being symptomatic. In addition to the six patients with hemorrhages during the DLT monitoring period, 10 experienced ITH (eight patients were symptomatic) thereafter. The recommended phase II dose for brainstem gliomas was 265 mg/m(2). Three of 27 patients with brainstem gliomas with imaging before and after irradiation, prior to receiving imatinib, had new hemorrhage, excluding their receiving imatinib. The MTD for recurrent high-grade gliomas without EIACDs was 465 mg/m(2), but the MTD was not established with EIACDs, with no DLTs at 800 mg/m(2). In summary, recommended phase II imatinib doses were determined for children with newly diagnosed brainstem glioma and recurrent high-grade glioma who were not receiving EIACDs. Imatinib may increase the risk of ITH, although the incidence of spontaneous hemorrhages in brainstem glioma is sufficiently high that this should be considered in studies of agents in which hemorrhage is a concern. C1 Childrens Hosp Pittsburgh, Dept Neurosurg, Pittsburgh, PA 15213 USA. Baylor Coll Med, Texas Childrens Canc Ctr, Houston, TX 77030 USA. Pediat Brain Tumor Consortium, Operat & Biostat Ctr, Memphis, TN 38105 USA. Dana Farber Canc Inst, Boston, MA 02115 USA. Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. St Jude Childrens Hosp, Memphis, TN 38105 USA. Duke Univ, Med Ctr, Durham, NC 27710 USA. Childrens Natl Med Ctr, Washington, DC 20010 USA. Univ Calif San Francisco, San Francisco, CA 94143 USA. Childrens Hosp & Med Ctr, Seattle, WA 98105 USA. Childrens Mem Hosp, Chicago, IL 60614 USA. Childrens Hosp, Boston, MA 02115 USA. Novartis Pharmaceut Corp, E Hanover, NJ 07936 USA. NCI, Bethesda, MD 20892 USA. Childrens Cause Canc Advocacy, Silver Spring, MD 20910 USA. RP Pollack, IF (reprint author), Childrens Hosp Pittsburgh, Dept Neurosurg, 3705 5th Ave, Pittsburgh, PA 15213 USA. EM ian.pollack@chp.edu OI Kieran, Mark/0000-0003-2184-7692 FU NCI NIH HHS [U01 CA081457, U01 CA81457]; NCRR NIH HHS [M01 RR000188, M01 RR00188-37] NR 51 TC 95 Z9 96 U1 0 U2 1 PU DUKE UNIV PRESS PI DURHAM PA 905 W MAIN ST, STE 18-B, DURHAM, NC 27701 USA SN 1522-8517 J9 NEURO-ONCOLOGY JI Neuro-Oncology PD APR PY 2007 VL 9 IS 2 BP 145 EP 160 DI 10.1215/15228517-2006-031 PG 16 WC Oncology; Clinical Neurology SC Oncology; Neurosciences & Neurology GA 154EJ UT WOS:000245489300009 PM 17293590 ER PT J AU Fouladi, M Blaney, SM Poussaint, TY Freeman, BB McLendon, R Fuller, C Adesina, AM Hancock, ML Remack, JS Hunt, D Danks, MK Ivy, P Stewart, C Kun, LE Gajjar, A AF Fouladi, M. Blaney, S. M. Poussaint, T. Y. Freeman, B. B., III McLendon, R. Fuller, C. Adesina, A. M. Hancock, M. L. Remack, J. S. Hunt, D. Danks, M. K. Ivy, P. Stewart, C. Kun, L. E. Gajjar, A. TI A phase II study of oxaliplatin in children with recurrent or refractory medulloblastoma, supratentorial primitive neuroectodermal tumors, and atypical teratoid rhabdoid tumors: A pediatric brain tumor consortium study SO NEURO-ONCOLOGY LA English DT Meeting Abstract CT 12th International Symposium on Pediatric Neuro-Oncology CY JUL 06-09, 2006 CL Nara, JAPAN C1 St Jude Childrens Hosp, Memphis, TN 38105 USA. Texas Childrens Canc Ctr, Houston, TX USA. Childrens Hosp, Boston, MA 02115 USA. Duke Univ, Durham, NC USA. NCI, Canc Therapy Evaluat Program, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU DUKE UNIV PRESS PI DURHAM PA 905 W MAIN ST, STE 18-B, DURHAM, NC 27701 USA SN 1522-8517 J9 NEURO-ONCOLOGY JI Neuro-Oncology PD APR PY 2007 VL 9 IS 2 BP 191 EP 192 PG 2 WC Oncology; Clinical Neurology SC Oncology; Neurosciences & Neurology GA 154EJ UT WOS:000245489300110 ER PT J AU van Hagen, JM van der Geest, JN van der Giessen, RS Lagers-van Haselen, GC Eussen, HJFMM Gille, JJP Govaerts, LCP Wouters, CH de Coo, IFM Hoogenraad, CC Koekkoek, SKE Frens, MA van Camp, N van der Linden, A Jansweijer, MCE Thorgeirsson, SS De Zeeuw, CI AF van Hagen, J. M. van der Geest, J. N. van der Giessen, R. S. Lagers-van Haselen, G. C. Eussen, H. J. F. M. M. Gille, J. J. P. Govaerts, L. C. P. Wouters, C. H. de Coo, I. F. M. Hoogenraad, C. C. Koekkoek, S. K. E. Frens, M. A. van Camp, N. van der Linden, A. Jansweijer, M. C. E. Thorgeirsson, S. S. De Zeeuw, C. I. TI Contribution of CYLN2 and GTF2IRD1 to neurological and cognitive symptoms in Williams Syndrome SO NEUROBIOLOGY OF DISEASE LA English DT Article DE human; mice; motor behavior; cognition; genetic disorder ID ATYPICAL 7Q11.23 DELETION; SYNDROME CRITICAL REGION; RECORDING EYE-MOVEMENTS; BEUREN-SYNDROME; CHROMOSOME 7Q11.23; MOUSE-BRAIN; CEREBELLAR; MICE; CHILDREN; PROFILE AB Williams Syndrome (WS, [MIM 194050]) is a disorder caused by a hemizygous deletion of 25-30 genes on chromosome 7q11.23. Several of these genes including those encoding cytoplasmic linker protein-115 (CYLN2) and general transcription factors (GTF2I and GTF2IRD1) are expressed in the brain and may contribute to the distinct neurological and cognitive deficits in WS patients. Recent studies of patients with partial deletions indicate that hemizygosity of GTF2I probably contributes to mental retardation in WS. Here we investigate whether CYLN2 and GTF2IRD1 contribute to the motoric and cognitive deficits in WS. Behavioral assessment of a new patient in which STX1A and LIMK1, but not CYLN2 and GTF2IRD1, are deleted showed that his cognitive and motor coordination functions were significantly better than in typical WS patients. Comparative analyses of gene specific CYLN2 and GTF2IRD1 knockout mice showed that a reduced size of the corpus callosum as well as deficits in motor coordination and hippocampal memory formation may be attributed to a deletion of CYLN2, while increased ventricle volume can be attributed to both CYLN2 and GTF2IRD1. We conclude that the motor and cognitive deficits in Williams Syndrome are caused by a variety of genes and that heterozygous deletion of CYLN2 is one of the major causes responsible for such dysfunctions. (c) 2006 Elsevier Inc. All rights reserved. C1 Erasmus MC, Dept Neurosci, NL-3000 CA Rotterdam, Netherlands. Erasmus MC, Dept Clin Genet, NL-3000 CA Rotterdam, Netherlands. Erasmus MC, Dept Child Neurol, NL-3000 CA Rotterdam, Netherlands. Univ Antwerp, Bioimaging Lab, B-2020 Antwerp, Belgium. Acad Med Ctr, Dept Pediat, NL-1100 DE Amsterdam, Netherlands. NCI, Expt Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. Vrije Univ Amsterdam, Med Ctr, Dept Clin Genet & Human Genet, NL-1007 MB Amsterdam, Netherlands. RP van der Geest, JN (reprint author), Erasmus MC, Dept Neurosci, POB 2040, NL-3000 CA Rotterdam, Netherlands. EM j.vandergeest@erasmusmc.nl RI Hoogenraad, Casper/B-8866-2011 OI Hoogenraad, Casper/0000-0002-2666-0758 NR 61 TC 35 Z9 36 U1 1 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0969-9961 J9 NEUROBIOL DIS JI Neurobiol. Dis. PD APR PY 2007 VL 26 IS 1 BP 112 EP 124 DI 10.1016/j.nbd.2006.12.009 PG 13 WC Neurosciences SC Neurosciences & Neurology GA 152PL UT WOS:000245374000012 PM 17270452 ER PT J AU Halagappa, VKM Guo, ZH Pearson, M Matsuoka, Y Cutler, RG LaFerla, FM Mattson, MP AF Halagappa, Veerendra Kumar Madald Guo, Zhihong Pearson, Michelle Matsuoka, Yasuji Cutler, Roy G. LaFerla, Frank M. Mattson, Mark P. TI Intermittent fasting and caloric restriction ameliorate age-related behavioral deficits in the triple-transgenic mouse model of Alzheimer's disease SO NEUROBIOLOGY OF DISEASE LA English DT Article DE amyloid; caloric restriction; hippocampus; learning and memory; synaptic plasticity ID MILD COGNITIVE IMPAIRMENT; AMYLOID BETA-PEPTIDE; DIETARY RESTRICTION; A-BETA; NEUROTROPHIC FACTOR; GLUTAMATE TRANSPORT; PARKINSONS-DISEASE; GLUCOSE-METABOLISM; DIABETES-MELLITUS; OXIDATIVE DAMAGE AB Alzheimer's disease (AD) is a neurodegenerative disorder characterized by progressive decline in cognitive function associated with the neuropathological hallmarks amyloid beta-peptide (A beta) plaques and neurolibrillary tangles. Because aging is the major risk factor for AD, and dietary energy restriction can retard aging processes in the brain, we tested the hypothesis that two different energy restriction regimens, 40% calorie restriction (CR) and intermittent fasting (IF) can protect against cognitive decline in the triple-transgenic mouse model of AD (3xTgAD mice). Groups of 3xTgAD mice were maintained on an ad libitum control diet, or CR or IF diets, beginning at 3 months of age. Half of the mice in each diet group were subjected to behavioral testing (Morris swim task and open field apparatus) at 10 months of age and the other half at 17 months of age. At 10 months 3xTgAD mice on the control diet exhibited reduced exploratory activity compared to non-transgenic mice and to 3xTgAD mice on CR and IF diets. Overall, there were no major differences in performance in the water maze among genotypes or diets in 10-month-old mice. In 17-month-old 3xTgAD mice the CR and IF groups exhibited higher levels of exploratory behavior, and performed better in both the goal latency and probe trials of the swim task, compared to 3xTgAD mice on the control diet. 3xTgAD mice in the CR group showed lower levels of A beta 1-40, A beta 1-42 and phospho-tau in the hippocampus compared to the control diet group, whereas A beta and phospho-tau levels were not decreased in 3xTgAD mice in the IF group. IF may therefore protect neurons against adverse effects of A beta and tau pathologies on synaptic function. We conclude that CR and IF dietary regimens can ameliorate age-related deficits in cognitive function by mechanisms that may or may not be related to A beta and tau pathologies. (c) 2007 Elsevier Inc. All rights reserved. C1 NIA, Neurosci Lab, Intramural Res Program, Baltimore, MD 21224 USA. Georgetown Univ, Med Ctr, Dept Neurol, Washington, DC 20057 USA. Univ Calif Irvine, Dept Neurobiol & Behav, Irvine, CA 92697 USA. RP Mattson, MP (reprint author), NIA, Neurosci Lab, Intramural Res Program, Baltimore, MD 21224 USA. EM mattsonm@grc.nia.nih.gov RI Mattson, Mark/F-6038-2012 FU Intramural NIH HHS; NIA NIH HHS [AG022455] NR 59 TC 201 Z9 206 U1 9 U2 32 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0969-9961 J9 NEUROBIOL DIS JI Neurobiol. Dis. PD APR PY 2007 VL 26 IS 1 BP 212 EP 220 DI 10.1016/j.nbd.2006.12.019 PG 9 WC Neurosciences SC Neurosciences & Neurology GA 152PL UT WOS:000245374000021 PM 17306982 ER PT J AU Mattson, MP AF Mattson, Mark P. TI Mitochondrial regulation of neuronal plasticity SO NEUROCHEMICAL RESEARCH LA English DT Review DE axon; calcium; dendrites; growth cone; hippocampus; learning and memory; psychiatric disorders ID LONG-TERM POTENTIATION; AMYLOID BETA-PEPTIDE; STABILIZING CALCIUM HOMEOSTASIS; PERMEABILITY TRANSITION PORE; SYNAPTIC PLASTICITY; ENERGY-METABOLISM; LIPID-PEROXIDATION; CORTICAL-NEURONS; HIPPOCAMPAL-NEURONS; COMPLEX-I AB The structure and function of neurons is dynamic during development and in adaptive responses of the adult nervous system to environmental demands. The mechanisms that regulate neuronal plasticity are poorly understood, but are believed to involve neurotransmitter and neurotrophic factor signaling pathways. In the present article, I review emerging evidence that mitochondria play important roles in regulating developmental and adult neuroplasticity. In neurons, mitochondria are located in axons, dendrites, growth cones and pre- and post-synaptic terminals where their movements and functions are regulated by local signals such as neurotrophic factors and calcium influx. Mitochondria play important roles in fundamental developmental processes including the establishment of axonal polarity and the regulation of neurite outgrowth, and are also involved in synaptic plasticity in the mature nervous system. Abnormalities in mitochondria are associated with neurodegenerative and psychiatric disorders, suggesting a therapeutic potential for approaches that target mitochondrial mechanisms. C1 NIA, Neurosci Lab, Intramural Res Program, Baltimore, MD 21224 USA. RP Mattson, MP (reprint author), NIA, Neurosci Lab, Intramural Res Program, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA. EM mattsonm@grc.nia.nih.gov RI Mattson, Mark/F-6038-2012 FU Intramural NIH HHS NR 86 TC 63 Z9 63 U1 0 U2 7 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-3190 J9 NEUROCHEM RES JI Neurochem. Res. PD APR PY 2007 VL 32 IS 4-5 BP 707 EP 715 DI 10.1007/s11064-006-9170-3 PG 9 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA 149GG UT WOS:000245134500017 PM 17024568 ER PT J AU Hardy, J AF Hardy, John TI Does A beta 42 have a function related to blood homeostasis? SO NEUROCHEMICAL RESEARCH LA English DT Review DE genetics; amyloid; Alzheimer's disease ID CEREBRAL AMYLOID ANGIOPATHY; BETA-PROTEIN PRECURSOR; ALZHEIMERS-DISEASE; TRANSGENIC MICE; SENILE PLAQUES; HYPOTHESIS; HEMORRHAGE; APP; DEMENTIA; TAU AB In this review, I discuss the possibility that A beta 42 has a physiologic function in blood vessel homeostasis and the consequences that this might have for theories concerning the pathogenesis of Alzheimer's disease and for treatment. C1 NIA, Neurogenet Lab, NIH, Bethesda, MD 20892 USA. RP Hardy, J (reprint author), NIA, Neurogenet Lab, NIH, Porter Neurosci Bldg,Main Campus, Bethesda, MD 20892 USA. EM hardyj@mail.nih.gov RI Hardy, John/C-2451-2009 FU Intramural NIH HHS; Medical Research Council [G0701075] NR 24 TC 14 Z9 14 U1 0 U2 0 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-3190 J9 NEUROCHEM RES JI Neurochem. Res. PD APR PY 2007 VL 32 IS 4-5 BP 833 EP 835 DI 10.1007/s11064-006-9221-9 PG 3 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA 149GG UT WOS:000245134500029 PM 17186373 ER PT J AU Brown, AM Gordon, D Lee, H Vrieze, FWD Cellini, E Bagnoli, S Nacmias, B Sorbi, S Hardy, J Blass, JP AF Brown, Abraham M. Gordon, Derek Lee, Hsinhwa Vrieze, Fabienne Wavrant-De Cellini, Elena Bagnoli, Silvia Nacmias, Benedetta Sorbi, Sandro Hardy, John Blass, John P. TI Testing for linkage and association across the dihydrolipoyl dehydrogenase gene region with Alzheimer's disease in three sample populations SO NEUROCHEMICAL RESEARCH LA English DT Article DE energy metabolism; mitochondria; gene; Alzheimer's disease; polymorphism; M-test; heterogeneity; diagnostic error; diagnosis; misclassification ID HARDY-WEINBERG EQUILIBRIUM; INTERNATIONAL HAPMAP PROJECT; GENOTYPING ERRORS; SIZE CALCULATIONS; APOLIPOPROTEIN-E; COMPLEX ACTIVITY; TYPE-4 ALLELE; HUMAN GENOME; POWER; DISEQUILIBRIUM AB Prior case-control studies from our laboratory of a population enriched with individuals of Ashkenazi Jewish descent suggested that association exists between Alzheimer's disease (AD) and the chromosomal region near the DLD gene, which encodes the mitochondrial dihydrolipoamide dehydrogenase enzyme. In support of this finding, we found that linkage analysis restricted to autopsy-proven patients in the National Institute of Mental Health-National Cell Repository for Alzheimer's Disease (NIMH-NCRAD) Genetics Initiative pedigree data resulted in point-wise significant evidence for linkage (minimum p-value = 0.024) for a marker position close to the DLD locus. We now report case-control replication studies in two independent Caucasian series from the US and Italy, as well as a linkage analysis from the NIMH-NCRAD Genetics Initiative Database. Pair-wise analysis of the SNPs in the case-control series indicated there was strong linkage disequilibrium across the DLD locus in these populations, as previously reported. These findings suggest that testing for association of complex diseases with DLD locus should have considerable statistical power. Analysis of multi-locus genotypes or haplotypes based upon three SNP loci combined with results from our previous report provided trends toward significant evidence of association of DLD with AD, although neither of the present studies' association showed significance at the 0.05 level. Combining linkage and association findings for all AD patients (males and females) results in a p-value that is more significant than any of the individual findings' p-values. Finally, minimum sample size calculations using parameters from the DLD locus suggest that sample sizes on the order of 1,000 total cases and controls are needed to detect association for a wide range of genetic model parameters. C1 Rutgers State Univ, Dept Genet, Piscataway, NJ 08854 USA. Cornell Univ, Coll Med, Burke Med Res Inst, White Plains, NY 10605 USA. Cornell Univ, Weill Med Coll, Dept Biochem, New York, NY 10021 USA. Natl Inst Neurol Dis & Stroke, Neurogenet Lab, Bethesda, MD 20892 USA. Dept Neurol & Psychiat Sci, I-50134 Florence, Italy. Cornell Univ, Weill Med Coll, Dept Neurol & Neurosci, New York, NY 10021 USA. Cornell Univ, Weill Med Coll, Dept Med, New York, NY 10021 USA. RP Gordon, D (reprint author), Rutgers State Univ, Dept Genet, Piscataway, NJ 08854 USA. EM gordon@biology.rutgers.edu RI Hardy, John/C-2451-2009; OI sorbi, sandro/0000-0002-0380-6670; NACMIAS, Benedetta/0000-0001-9338-9040 FU Medical Research Council [G0701075]; NIA NIH HHS [AG 14930, U24 AG021886]; NIMH NIH HHS [U01 MH 46281, U01 MH 46290, U01 MH 46373] NR 63 TC 3 Z9 3 U1 0 U2 3 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-3190 J9 NEUROCHEM RES JI Neurochem. Res. PD APR PY 2007 VL 32 IS 4-5 BP 857 EP 869 DI 10.1007/s11064-006-9235-3 PG 13 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA 149GG UT WOS:000245134500032 PM 17342416 ER PT J AU Hawes, JJ Tuskan, RG Reilly, KM AF Hawes, Jessica J. Tuskan, Robert G. Reilly, Karlyne M. TI Nf1 expression is dependent on strain background: implications for tumor suppressor haploinsufficiency studies SO NEUROGENETICS LA English DT Article DE neurofibromatosis; Nf1; haploinsufficiency; astrocytoma; peripheral nerve sheath tumor ID NEUROFIBROMATOSIS TYPE-1 GENE; IN-VITRO; CELL; PROLIFERATION; MICE; GAP; DIFFERENTIATION; HETEROZYGOSITY; ASTROCYTES; MUTATION AB Neurofibromatosis type 1 (NF1) is the most common cancer predisposition syndrome affecting the nervous system, with elevated risk for both astrocytoma and peripheral nerve sheath tumors. NF1 is caused by a germline mutation in the NF1 gene, with tumors showing loss of the wild type copy of NF1. In addition, NF1 heterozygosity in surrounding stroma is important for tumor formation, suggesting an additional role of haploinsufficiency for NF1. Studies in mouse models and NF1 families have implicated modifier genes unlinked to NF1 in the severity of the disease and in susceptibility to astrocytoma and peripheral nerve sheath tumors. To determine if differences in Nf1 expression may contribute to the strain-specific effects on tumor predisposition, we examined the levels of Nf1 gene expression in mouse strains with differences in tumor susceptibility using quantitative polymerase chain reaction. The data presented in this paper demonstrate that strain background has as much effect on Nf1 expression levels as mutation of one Nf1 allele, indicating that studies of haploinsufficiency must be carefully interpreted with respect to strain background. Because expression levels do not correlate entirely with the susceptibility or resistance to tumors observed in the strain, these data suggest that either variation in Nf1 levels is not responsible for the differences in astrocytoma and peripheral nerve sheath tumor susceptibility in Nf1-/+;Trp53-/+cis mice, or that certain mouse strains have evolved compensatory mechanisms for differences in Nf1 expression. C1 NCI, Mouse Canc Genet Program, Frederick, MD 21702 USA. RP Reilly, KM (reprint author), NCI, Mouse Canc Genet Program, W 7th St & Ft Detrick,POB B,Bldg 560,Rm 31-32B, Frederick, MD 21702 USA. EM kreilly@ncifcrf.gov FU Intramural NIH HHS; NIDA NIH HHS [P20-DA 21131]; NIMH NIH HHS [P20-MH 62009] NR 33 TC 19 Z9 19 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 1364-6745 J9 NEUROGENETICS JI Neurogenetics PD APR PY 2007 VL 8 IS 2 BP 121 EP 130 DI 10.1007/s10048-006-0078-5 PG 10 WC Genetics & Heredity; Clinical Neurology SC Genetics & Heredity; Neurosciences & Neurology GA 143AM UT WOS:000244692400006 PM 17216419 ER PT J AU Pannacciulli, N Le, DSNT Salbe, AD Chen, KW Reiman, EM Tataranni, PA Krakoff, J AF Pannacciulli, Nicola Le, Due Son N. T. Salbe, Arline D. Chen, Kewei Reiman, Eric M. Tataranni, Pietro A. Krakoff, Jonathan TI Postprandial glucagon-like peptide-1 (GLP-1) response is positively associated with changes in neuronal activity of brain areas implicated in satiety and food intake regulation in humans SO NEUROIMAGE LA English DT Article ID POSITRON-EMISSION-TOMOGRAPHY; ORBITOFRONTAL CORTEX; BAYESIAN-INFERENCE; INCRETIN HORMONES; PREFRONTAL CORTEX; HUMAN AMYGDALA; OBESE SUBJECTS; NORMAL-WEIGHT; HUNGER; SATIATION AB Postprandial glucagon-like peptide-1 (GLP-1) secretion can act as a meal termination signal in animals and humans. We tested the hypothesis that the postprandial changes in plasma GLP-1 concentrations are associated with changes in the human brain activity in response to satiety by performing a post-hoc analysis of a cross-sectional study of neuroanatomical correlates of hunger and satiation using O-15-water positron-emission tomography (PET). Forty-two subjects (22M/201F, age 31 +/- 8 years) spanning a wide range of adiposity (body fat: 7-44%) were included in this analysis. Outcome measures included changes in PET-measured regional cerebral blood flow (rCBF) and plasma concentrations of GLP-1, glucose, insulin, and free-fatty acids (FFA), elicited by the administration of a satiating amount of a liquid formula meal. The peak postprandial increases in plasma GLP-1 concentrations were correlated with increases in rCBF in the left dorsolateral prefrontal cortex (including the left middle and inferior frontal gyri), previously implicated in PET studies of human satiation, and the hypothalamus, previously implicated in the regulation of food intake in animal and human studies, both before and after adjustment for sex, age, body fat, and changes in plasma glucose, insulin, and serum FFA concentrations. The postprandial GLP-1 response is associated with activation of areas of the human brain previously implicated in satiation and food intake regulation. (c) 2007 Elsevier Inc. All rights reserved. C1 NIDDK, Obes & Diabet Clin Res Sect, NIH, DHHS, Phoenix, AZ 85016 USA. Banner Good Samaritan Med Ctr, Banner Alzheimer Inst, Phoenix, AZ USA. Banner Good Samaritan Med Ctr, Banner Good Samaritan Positron Emiss Tomog Ctr, Phoenix, AZ USA. Univ Arizona, Dept Psychiat, Phoenix, AZ USA. Translat Genom Res Inst, Neurogenom Program, Phoenix, AZ USA. RP Pannacciulli, N (reprint author), NIDDK, Obes & Diabet Clin Res Sect, NIH, DHHS, 4212 N 16th St, Phoenix, AZ 85016 USA. EM nicolap@mail.nih.gov RI Chen, kewei/P-6304-2015 OI Chen, kewei/0000-0001-8497-3069 FU Intramural NIH HHS [Z99 DK999999] NR 42 TC 45 Z9 52 U1 1 U2 7 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1053-8119 J9 NEUROIMAGE JI Neuroimage PD APR 1 PY 2007 VL 35 IS 2 BP 511 EP 517 DI 10.1016/j.neuroimage.2006.12.035 PG 7 WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA 151LY UT WOS:000245293100007 PM 17317222 ER PT J AU Marsh, AA Blair, KS Vythilingam, M Busis, S Blair, RJR AF Marsh, Abigail A. Blair, Karina S. Vythilingam, Meena Busis, Sarah Blair, R. J. R. TI Response options and expectations of reward in decision-making: The differential roles of dorsal and rostral anterior singulate cortex SO NEUROIMAGE LA English DT Article DE decision; FMRI; anterior cingulate; caudate; medial prefrontal cortex; amygdala ID HUMAN ORBITOFRONTAL CORTEX; PREFRONTAL CORTEX; CINGULATE CORTEX; COGNITIVE CONTROL; NEURAL REPRESENTATION; BASOLATERAL AMYGDALA; EXECUTIVE PROCESSES; VISUAL COMPLEXITY; EXPECTED OUTCOMES; FUNCTIONAL MRI AB This study examined the functional specificity of dorsal anterior cingulate cortex (dACC) and rostral anterior cingulate cortex (rACC)/medial prefrontal cortex (mPFC) regarding two elements of decision-making: the number of available decision options and the level of expected reward. Eighteen healthy participants were trained to recognize the reward value associated with several visual stimuli, and then were presented with groups of two, three, or four of these stimuli and asked to select the object associated with the highest reward. BOLD activation in dACC/dorsomedial frontal cortex (dmFC) was strongly positively associated with increases in the number of decision options but only weakly associated with increases in the level of expected reward. Activation in rACC/mPFC and amygdala was related to increases in the level of expected reward but not increases in the number of decision options. The current results suggest functional specificity with respect to the roles of dACC/dmFC and rACC/mPFC in decision-making. Published by Elsevier Inc. C1 NIMH, Mood & Anxiety Program, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Marsh, AA (reprint author), NIMH, Mood & Anxiety Program, NIH, Dept Hlth & Human Serv, 15K N Dr,MSC 2670, Bethesda, MD 20892 USA. EM amarsh@post.harvard.edu FU Intramural NIH HHS [Z99 MH999999] NR 58 TC 41 Z9 42 U1 2 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1053-8119 J9 NEUROIMAGE JI Neuroimage PD APR 1 PY 2007 VL 35 IS 2 BP 979 EP 988 DI 10.1016/j.neuroimage.2006.11.044 PG 10 WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA 151LY UT WOS:000245293100048 PM 17292631 ER PT J AU Du, J Suzuki, K Wei, Y Wang, Y Blumenthal, R Chen, Z Falke, C Zarate, CA Manji, HK AF Du, Jing Suzuki, Katsuji Wei, Yanling Wang, Yun Blumenthal, Rayah Chen, Zheng Falke, Cynthia Zarate, Carlos A., Jr. Manji, Husseini K. TI The anticonvulsants lamotrigine, riluzole, and valproate differentially regulate AMPA receptor membrane localization: Relationship to clinical effects in mood disorders SO NEUROPSYCHOPHARMACOLOGY LA English DT Article DE AMPA receptors; phosphorylation; lamotrigine; riluzole; valproate; imipramine ID DEPENDENT PROTEIN-KINASE; OPEN-LABEL TRIAL; SYNAPTIC PLASTICITY; GLUR1 SUBUNIT; PHOSPHORYLATION SITES; SERUM CONCENTRATIONS; ANTIEPILEPTIC DRUGS; BIPOLAR DISORDER; ANTIMANIC AGENTS; GLUTAMATE AB A growing body of data suggests that the glutamatergic system may be involved in the pathophysiology and treatment of severe mood disorders. Chronic treatment with the antimanic agents, lithium and valproate, resulted in reduced synaptic expression of the AMPA(-amino-3-hydroxy-5-methylisoxazole-4-propionic acid) receptor subunit GluR1 in the hippocampus, while treatment with an antidepressant (imipramine) enhanced the synaptic expression of GluR1. The anticonvulsants, lamotrigine (6-(2,3-dichlorophenyl)-1,2,4-triazine-3,5-diamine) and riluzole (2-amino-6-trifluoromethoxybenzothiazole), have been demonstrated to have efficacy in the depressive phase of bipolar disorder. We therefore sought to determine the role of these anticonvulsants, compared to that of the predominantly antimanic anticonvulsant valproate, on AMPA receptor localization. We found that the agents with a predominantly antidepressant profile, namely lamotrigine and riluzole, significantly enhanced the surface expression of GluR1 and GluR2 in a time- and dose-dependent manner in cultured hippocampal neurons. By contrast, the predominantly antimanic agent, valproate, significantly reduced surface expression of GluR1 and GluR2. Concomitant with the GluR1 and GluR2 changes, the peak value of depolarized membrane potential evoked by AMPA was significantly higher in lamotrigine- and riluzole-treated neurons, supporting the surface receptor changes. Phosphorylation of GluR1 at the PKA (cAMP-dependent protein kinase) site (S845) was enhanced in both lamotrigine- and riluzole-treated hippocampal neurons, but reduced in valproate-treated neurons. In addition, lamotrigine and riluzole, as well as the traditional antidepressant imipramine, also increased GluR1 phosphorylation at GluR1 (S845) in the hippocampus after chronic in vivo treatment. Our findings suggest that regulation of GluR1/2 surface levels and function may be responsible for the different clinical profile of anticonvulsants (antimanic or antidepressant), and may suggest avenues for the development of novel therapeutics for these illnesses. C1 NIMH, Mol Pathophysiol Lab, Mood & Anxiety Disorders Program, NIH, Bethesda, MD 20892 USA. RP Manji, HK (reprint author), NIMH, Mol Pathophysiol Lab, Mood & Anxiety Disorders Program, NIH, 9000 Rockville Pike,Bldg 35,1C912, Bethesda, MD 20892 USA. EM manjih@mail.nih.gov RI Wang, Yu Tian/A-4729-2008 FU Intramural NIH HHS NR 52 TC 117 Z9 122 U1 1 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0893-133X J9 NEUROPSYCHOPHARMACOL JI Neuropsychopharmacology PD APR PY 2007 VL 32 IS 4 BP 793 EP 802 DI 10.1038/sj.npp.1301178 PG 10 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 146SV UT WOS:000244955900006 PM 16936714 ER PT J AU Insel, TR Stover, E AF Insel, Thomas R. Stover, Ellen TI Wayne Fenton, 1953-2006 - Obituary SO NEUROPSYCHOPHARMACOLOGY LA English DT Biographical-Item ID SCHIZOPHRENIA SUBTYPES; NATURAL-HISTORY C1 NIMH, Bethesda, MD 20892 USA. RP Insel, TR (reprint author), NIMH, Bethesda, MD 20892 USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0893-133X J9 NEUROPSYCHOPHARMACOL JI Neuropsychopharmacology PD APR PY 2007 VL 32 IS 4 BP 973 EP 973 DI 10.1038/sj.npp.1301299 PG 1 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 146SV UT WOS:000244955900024 ER PT J AU Mizuhiki, T Richmond, BJ Shidara, M AF Mizuhiki, Takashi Richmond, Baffy J. Shidara, Munetaka TI Mode changes in activity of single neurons in anterior insular cortex across trials during multi-trial reward schedules SO NEUROSCIENCE RESEARCH LA English DT Article DE single unit; spike count; Poisson distribution; neural coding; response variability; rhesus monkey; reward expectancy ID SIGNALS; MONKEY; CINGULATE; STATES AB We previously showed that spike count response distributions in anterior cingulate neurons can be fitted by a mixture of a few Poisson distributions in our reward schedule task. Here we report that the neuronal responses in insular cortex, an area connected to anterior cingulate cortex, can also be nicely fitted. The ratio of Poisson distributions changed with schedule progress, suggesting that neuronal responses in these areas fall into discrete firing modes. More insular neurons show mode changes across the schedules. The selection of firing modes might be related to cognitive processes, but seems independent across the two areas. (c) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. C1 Univ Tsukuba, Grad Sch Comprehens Human Sci, Tsukuba, Ibaraki 3058577, Japan. Natl Inst Adv Ind Sci & Technol, Neurosci Res Inst, Tsukuba, Ibaraki 3058568, Japan. NIMH, Neuropsychol Lab, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Shidara, M (reprint author), Univ Tsukuba, Grad Sch Comprehens Human Sci, 1-1-1 Amakubo, Tsukuba, Ibaraki 3058577, Japan. EM mshidara@md.tsukuba.ac.jp FU Intramural NIH HHS [Z99 MH999999] NR 14 TC 2 Z9 2 U1 0 U2 1 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-0102 J9 NEUROSCI RES JI Neurosci. Res. PD APR PY 2007 VL 57 IS 4 BP 587 EP 591 DI 10.1016/j.neures.2006.12.008 PG 5 WC Neurosciences SC Neurosciences & Neurology GA 158BN UT WOS:000245766000014 PM 17257703 ER PT J AU Neuwelt, EA Varallyay, CG Manninger, S Solymosi, D Haluska, M Hunt, MA Nesbit, G Stevens, A Jerosch-Herold, M Jacobs, PM Hoffman, JM AF Neuwelt, Edward A. Varallyay, Csanad G. Manninger, Sandor Solymosi, Diana Haluska, Marianne Hunt, Matthew A. Nesbit, Gary Stevens, Alexander Jerosch-Herold, Michael Jacobs, Paula M. Hoffman, John M. TI The potential of ferumoxytol nanoparticle magnetic resonance imaging, perfusion, and angiograpgy in central nervous system malignancy: A pilot study SO NEUROSURGERY LA English DT Article DE brain tumor; contrast agent; ferumoxytol; magnetic resonance angiography; magnetic resonance; imaging; magnetic resonance perfusion; ultrasmall superparamagnetic iron oxide nanoparticles ID SUPERPARAMAGNETIC IRON-OXIDE; MULTICENTER CLINICAL-TRIAL; LYMPH-NODE METASTASES; HIGH-GRADE GLIOMAS; BRAIN-TUMORS; CONTRAST AGENT; INTRACRANIAL HEMATOMAS; MR-ANGIOGRAPHY; TIME; BLOOD AB OBJECTIVE: Ferumoxytol, an iron oxide nanoparticle that targets phagocytic cells, can be used in magnetic resonance imaging of malignant brain tumors and can be administered as a bolus, allowing dynamic imaging. Our objectives were to determine the optimum time of delayed contrast enhancement of ferumoxytol, and to compare ferumoxytol and gadolinium contrast agents for magnetic resonance angiography and perfusion. METHODS: Twelve patients with malignant brain tumors underwent serial magnetic resonance imaging multiple times up to 72 hours after ferumoxytol injection at both 1.5 and 3-T. The enhancement time course was determined for ferumoxytol and compared with a baseline gadolinium scan. Perfusion, time-of-flight and dynamic magnetic resonance angiography and T1-weighted scans were compared for the two agents. RESULTS: The lesions were detectable at all field strengths, even with an intraoperative 0.15-T magnet. Maximal ferumoxytol enhancement intensity was at 24 to 28 hours after administration, and the enhancing volume subsequently expanded with time into a non-gadolinium-enhancing, high T2-weighted signal region of tumor-infiltrated brain. Dynamic studies were assessed with both agents, indicating early vascular leak with gadolinium but not with ferumoxytol. CONCLUSION: Our most important finding was that gadolinium leaks out of blood vessels early after injection, whereas ferumoxytol stays intravascular in the "early" phase, thereby increasing the accuracy of tumor perfusion assessment. As a magnetic resonance imaging contrast agent, ferumoxytol visualizes brain tumors at all field strengths evaluated, with delayed enhancement peaking at 24 to 28 hours after administration. C1 Oregon Hlth & Sci Univ, Dept Neurol, Portland, OR 97239 USA. Oregon Hlth & Sci Univ, Dept Neurosurg, Portland, OR 97239 USA. Vet Adm Med Ctr, Portland, OR USA. Oregon Hlth & Sci Univ, Dept Radiol, Portland, OR 97239 USA. Oregon Hlth & Sci Univ, Dept Psychiat, Portland, OR 97239 USA. Oregon Hlth & Sci Univ, Adv Imaging Res Ctr, Portland, OR 97239 USA. NCI, Canc Imaging Program, Bethesda, MD 20892 USA. SAIC Frederick Inc, Frederick, MD USA. Univ Utah, Dept Radiol, Salt Lake City, UT 84132 USA. RP Neuwelt, EA (reprint author), Oregon Hlth & Sci Univ, Dept Neurol, 3181 SW Sam Jackson Pk Rd, Portland, OR 97239 USA. EM neuweile@ohsu.edu RI Jerosch-Herold, Michael/F-2496-2010 FU NCI NIH HHS [N01-CO-12400] NR 40 TC 85 Z9 86 U1 1 U2 5 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0148-396X J9 NEUROSURGERY JI Neurosurgery PD APR PY 2007 VL 60 IS 4 BP 601 EP 611 PG 11 WC Clinical Neurology; Surgery SC Neurosciences & Neurology; Surgery GA 153YD UT WOS:000245473100013 PM 17415196 ER PT J AU Cadet, JL Krasnova, IN Jayanthi, S Lyles, J AF Cadet, Jean Lud Krasnova, Irina N. Jayanthi, Subramaniam Lyles, Johnalyn TI Neurotoxicity of substituted amphetamines: Molecular and cellular mechanisms SO NEUROTOXICITY RESEARCH LA English DT Review DE substituted amphetamines; methamphetamine; methylenedioxyamphetamine; MDMA; serotoninergic neurons; dopaminergic neurons; hyperthermia; neurotoxicity ID METHAMPHETAMINE-INDUCED NEUROTOXICITY; DISMUTASE TRANSGENIC MICE; INDUCED DOPAMINERGIC NEUROTOXICITY; INDUCED NEURONAL APOPTOSIS; ZINC SUPEROXIDE-DISMUTASE; CENTRAL-NERVOUS-SYSTEM; SEROTONIN TRANSPORTER DENSITY; POSITRON-EMISSION-TOMOGRAPHY; HYDROXYL RADICAL FORMATION; MDMA-INDUCED NEUROTOXICITY AB The amphetamines, including amphetamine (AMPH), methamphetamine (METH) and 3,4-methylenedioxymethamphetamine (MDMA), are among abused drugs in the US and throughout the world. Their abuse is associated with severe neurologic and psychiatric adverse events including the development of psychotic states. These neuropsychiatric complications might, in part, be related to drug-induced neurotoxic effects, which include damage to dopaminergic and serotonergic terminals, neuronal apoptosis, as well as activated astroglial and microglial cells in the brain. The purpose of the present review is to summarize the toxic effects of AMPH, METH and MDMA. The paper also presents some of the factors that are thought to underlie this toxicity. These include oxidative stress, hyperthermia, excitotoxicity and various apoptotic pathways. Better understanding of the cellular and molecular mechanisms involved in their toxicity should help to generate modern therapeutic approaches to prevent or attenuate the long-term consequences of amphetamine use disorders in humans. C1 DHHS NIH NIDA, Intramural Res Program, Mol Neuropsychiat Branch, Baltimore, MD 21224 USA. RP Cadet, JL (reprint author), DHHS NIH NIDA, Intramural Res Program, Mol Neuropsychiat Branch, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM jcadet@intra.nida.nih.gov NR 249 TC 157 Z9 158 U1 6 U2 27 PU F P GRAHAM PUBLISHING CO PI MOUNTAIN HOME PA PO BOX 370, MOUNTAIN HOME, TN 37684 USA SN 1029-8428 J9 NEUROTOX RES JI Neurotox. Res. PD APR PY 2007 VL 11 IS 3-4 BP 183 EP 202 PG 20 WC Neurosciences SC Neurosciences & Neurology GA 180QK UT WOS:000247380400004 PM 17449459 ER PT J AU Sorger, D Becker, GA Patt, M Schildan, A Grossmann, U Schliebs, R Seese, A Kendziorra, K Kluge, M Brust, P Mukhin, AG Sabri, O AF Sorger, Dietlind Becker, Georg A. Patt, Marianne Schildan, Andreas Grossmann, Udo Schliebs, Reinhard Seese, Anita Kendziorra, Kai Kluge, Magnus Brust, Peter Mukhin, Alexey G. Sabri, Osama TI Measurement of the alpha 4 beta 2*nicotinic acetylcholine receptor ligand 2-[F-18]Fluoro-A-85380 and its metabolites in human blood during PET investigation: a methodological study SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article DE 2-[F-18]fluoro-A-85380; human PET studies; metabolites; HPLC separation; solid phase extraction; TLC ID POSITRON-EMISSION-TOMOGRAPHY; IN-VIVO; NICOTINIC RECEPTORS; BINDING-PROPERTIES; DOPAMINE RELEASE; HUMAN BRAIN; ALPHA-4-BETA-2; AFFINITY; SUBTYPES; DISEASE AB 2-[F-18]fluoro-A-85380 (2-[F-18]FA) is a new radioligand for noninvasive imaging of alpha 4 beta 2* nicotinic acetylcholine receptors (nAChRs) by positron emission tomography (PET) in human brain. In most cases, quantification of 2-[F-18]FA receptor binding involves measurement of free nonmetabolized radioligand concentration in blood. This requires an efficient and reliable method to separate radioactive metabolites from the parent compound. In the present study, three analytical methods, thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and solid phase extraction (SPE) have been tested. Reversed-phase TLC of deproteinized aqueous samples of plasma provides good estimates of 2-[F-18]FA and its metabolites. However, because of the decreased radioactivity in plasma samples, this method can be used in humans over the first 2 h after radioligand injection only. Reliable quantification of the parent radioligand and its main metabolites was obtained using reversed-phase HPLC, followed by counting of eluted fractions in a well gamma counter. Three main and five minor metabolites of 2-[F-18]FA were detected in human blood using this method. On average, the unchanged 2-[F-18]FA fraction in plasma of healthy volunteers measured at 14, 60, 120, 240 and 420 min after radioligand injection was 87.3 +/- 2.2%, 74.4 +/- 3%, 68.8 +/- 5%, 62.3 +/- 8% and 61.0 +/- 8%,. respectively. In patients with neurodegenerative disorders, the values corresponding to the three last time points were significantly lower. The fraction of nonmetabolized 2-[F-18]FA in plasma determined using SPE did not differ significantly from that obtained by HPLC (+gamma counting) (n=73, r=.95). Since SPE is less time-consuming than HPLC and provides comparable results, we conclude that SPE appears to be the most suitable method for measurement of 2-[F-18]FA parent fraction during PET investigations. (c) 2007 Elsevier Inc. All rights reserved. C1 Univ Leipzig, Dept Nucl Med, D-04103 Leipzig, Germany. Univ Leipzig, Paul Flechsig Inst Brain Res, D-04109 Leipzig, Germany. Univ Leipzig, Inst Interdisciplinary Isotope Res, D-04318 Leipzig, Germany. Natl Inst Durg Abuse, Neuroimaging Res Branch, NIH, Baltimore, MD 21224 USA. RP Sorger, D (reprint author), Univ Leipzig, Dept Nucl Med, D-04103 Leipzig, Germany. EM sord@medizin.uni-leipzig.de NR 37 TC 15 Z9 16 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0969-8051 J9 NUCL MED BIOL JI Nucl. Med. Biol. PD APR PY 2007 VL 34 IS 3 BP 331 EP 342 DI 10.1016/j.nucmedbio.2006.12.008 PG 12 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 154NH UT WOS:000245513800013 PM 17383583 ER PT J AU Ploquin, M Petukhova, GV Morneau, D Dery, U Bransi, A Stasiak, A Camerini-Otero, RD Masson, JY AF Ploquin, Mickael Petukhova, Galina V. Morneau, Dany Dery, Ugo Bransi, Ali Stasiak, Andrzej Camerini-Otero, R. Daniel Masson, Jean-Yves TI Stimulation of fission yeast and mouse Hop2-Mnd1 of the Dmc1 and Rad51 recombinases SO NUCLEIC ACIDS RESEARCH LA English DT Article ID REPLICATION PROTEIN-A; DNA STRAND EXCHANGE; MEIOTIC RECOMBINATION; SACCHAROMYCES-CEREVISIAE; SCHIZOSACCHAROMYCES-POMBE; HOMOLOGOUS RECOMBINATION; CHROMOSOME SYNAPSIS; COMPLEX-FORMATION; BUDDING YEAST; HOP2 PROTEIN AB Genetic analysis of fission yeast suggests a role for the spHop2-Mnd1 proteins in the Rad51 and Dmc1-dependent meiotic recombination pathways. In order to gain biochemical insights into this process, we purified Schizosaccharomyces pombe Hop2-Mnd1 to homogeneity. spHop2 and spMnd1 interact by co-immunoprecipitation and two-hybrid analysis. Electron microscopy reveals that S. pombe Hop2-Mnd1 binds single-strand DNA ends of 3 '-tailed DNA. Interestingly, spHop2-Mnd1 promotes the renaturation of complementary single-strand DNA and catalyses strand exchange reactions with short oligonucleotides. Importantly, we show that spHop2-Mnd1 stimulates spDmc1-dependent strand exchange and strand invasion. Ca2+ alleviate the requirement for the order of addition of the proteins on DNA. We also demonstrate that while spHop2-Mnd1 affects spDmc1 specifically, mHop2 or mHop2-Mnd1 stimulates both the hRad51 and hDmc1 recombinases in strand exchange assays. Thus, our results suggest a crucial role for S. pombe and mouse Hop2-Mnd1 in homologous pairing and strand exchange and reveal evolutionary divergence in their specificity for the Dmc1 and Rad51 recombinases. C1 Univ Laval, Hotel Dieu Quebec, Canc Res Ctr, Genome Stabil Lab, Quebec City, PQ G1R 2J6, Canada. NIDDK, Genet & Biochem Branch, NIH, Bethesda, MD 20892 USA. Univ Lausanne, Fac Biol & Med, Lab Ultrastruct Anal, CH-1015 Lausanne, Switzerland. RP Masson, JY (reprint author), Univ Laval, Hotel Dieu Quebec, Canc Res Ctr, Genome Stabil Lab, 9 McMahon, Quebec City, PQ G1R 2J6, Canada. EM Jean-Yves.Masson@crhdq.ulaval.ca RI Stasiak, Andrzej/E-5551-2010 NR 51 TC 26 Z9 30 U1 0 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 EI 1362-4962 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2007 VL 35 IS 8 BP 2719 EP 2733 DI 10.1093/nar/gkm174 PG 15 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178SE UT WOS:000247239600025 PM 17426123 ER PT J AU Mao, GG Pan, XY Zhu, BB Zhang, YB Yuan, FH Huang, J Lovell, MA Lee, MP Markesbery, WR Li, GM Gu, LY AF Mao, Guogen Pan, Xiaoyu Zhu, Bei-Bei Zhang, Yanbin Yuan, Fenghua Huang, Jian Lovell, Mark A. Lee, Maxwell P. Markesbery, William R. Li, Guo-Min Gu, Liya TI Identification and characterization of OGG1 mutations in patients with Alzheimer's disease SO NUCLEIC ACIDS RESEARCH LA English DT Article ID INCREASED OXIDATIVE DAMAGE; BASE EXCISION-REPAIR; MITOCHONDRIAL-DNA; STRUCTURAL BASIS; 8-HYDROXYGUANINE; 8-OXOGUANINE; MICE; ACCUMULATION; GLYCOSYLASE; NUCLEAR AB Patients with Alzheimer's disease (AD) exhibit higher levels of 8-oxo-guanine (8-oxoG) DNA lesions in their brain, suggesting a reduced or defective 8-oxoG repair. To test this hypothesis, this study investigated 14 AD patients and 10 age-matched controls for mutations of the major 8-oxoG removal gene OGG1. Whereas no alterations were detected in any control samples, four AD patients exhibited mutations in OGG1, two carried a common single base (C-796) deletion that alters the carboxyl terminal sequence of OGG1, and the other two had nucleotide alterations leading to single amino acid substitutions. In vitro biochemical assays revealed that the protein encoded by the C-796-deleted OGG1 completely lost its 8-oxoG glycosylase activity, and that the two single residue-substituted OGG1 proteins showed a significant reduction in the glycosylase activity. These results were consistent with the fact that nuclear extracts derived from a limited number of AD patients with OGG1 mutations exhibited greatly reduced 8-oxoG glycosylase activity compared with age-matched controls and AD patients without OGG1 alterations. Our findings suggest that defects in OGG1 may be important in the pathogenesis of AD in a significant fraction of AD patients and provide new insight into the molecular basis for the disease. C1 Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40506 USA. Univ Kentucky, Dept Pathol & Lab Med, Lexington, KY USA. Univ Kentucky, Sanders Brown Ctr Aging, Lexington, KY 40536 USA. Univ Kentucky, Dept Chem, Lexington, KY 40506 USA. Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China. NCI, Lab Populat Genet, Bethesda, MD 20892 USA. RP Gu, LY (reprint author), Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40506 USA. EM lgu0@uky.edu RI Zhang, Yanbin/F-2998-2011; Yuan, Fenghua/F-8736-2011; Li, Guo-Min/I-5016-2014; OI Zhang, Yanbin/0000-0002-7263-5510; Li, Guo-Min/0000-0002-9842-4578 FU NIA NIH HHS [P01 AG05119, P01 AG005119, 1P30 AG0 28383, P30 AG028383] NR 30 TC 59 Z9 61 U1 0 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2007 VL 35 IS 8 BP 2759 EP 2766 DI 10.1093/nar/gkm189 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178SE UT WOS:000247239600028 PM 17426120 ER PT J AU Chan, W Costantino, N Li, RX Lee, SC Su, Q Melvin, D Court, DL Liu, PT AF Chan, Waiin Costantino, Nina Li, Ruixue Lee, Song Choon Su, Qin Melvin, David Court, Donald L. Liu, Pentao TI A recombineering based approach for high-throughput conditional knockout targeting vector construction SO NUCLEIC ACIDS RESEARCH LA English DT Article ID ESCHERICHIA-COLI; MOUSE GENOME; HOMOLOGOUS RECOMBINATION; BACTERIOPHAGE-LAMBDA; MAMMALIAN-CELLS; DNA; CHROMOSOME; SYSTEM; TRANSCRIPTION; STEP AB Functional analysis of mammalian genes in vivo is primarily achieved through analysing knockout mice. Now that the sequencing of several mammalian genomes has been completed, understanding functions of all the genes represents the next major challenge in the post-genome era. Generation of knockout mutant mice has currently been achieved by many research groups but only by making individual knockouts, one by one. New technological advances and the refinements of existing technologies are critical for genome-wide targeted mutagenesis in the mouse. We describe here new recombineering reagents and protocols that enable recombineering to be carried out in a 96-well format. Consequently, we are able to construct 96 conditional knockout targeting vectors simultaneously. Our new recombineering system makes it a reality to generate large numbers of precisely engineered DNA constructs for functional genomics studies. C1 Wellcome Trust Sanger Inst, Cambridge CB10 1HH, England. NCI Frederick, Ft Detrick, MD 21702 USA. RP Court, DL (reprint author), Wellcome Trust Sanger Inst, Cambridge CB10 1HH, England. EM court@ncifcrf.gov; pl2@sanger.ac.uk FU Intramural NIH HHS; Wellcome Trust NR 36 TC 51 Z9 59 U1 1 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2007 VL 35 IS 8 AR e64 DI 10.1093/nar/gkm163 PG 13 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178SE UT WOS:000247239600042 PM 17426124 ER PT J AU Martin, SE Jones, TL Thomas, CL Lorenzi, PL Nguyen, DA Runfola, T Gunsior, M Weinstein, JN Goldsmith, PK Lader, E Huppi, K Caplen, NJ AF Martin, Scott E. Jones, Tamara L. Thomas, Cheryl L. Lorenzi, Philip L. Nguyen, Dac A. Runfola, Timothy Gunsior, Michele Weinstein, John N. Goldsmith, Paul K. Lader, Eric Huppi, Konrad Caplen, Natasha J. TI Multiplexing siRNAs to compress RNAi-based screen size in human cells SO NUCLEIC ACIDS RESEARCH LA English DT Article ID INTERFERING RNAS; MAMMALIAN-CELLS; SHRNA LIBRARIES; CANCER-THERAPY; GENETIC SCREEN; HUMAN KINASES; TARGET; MODULATORS; APOPTOSIS; PATHWAY AB Here we describe a novel strategy using multiplexes of synthetic small interfering RNAs (siRNAs) corresponding to multiple gene targets in order to compress RNA interference (RNAi) screen size. Before investigating the practical use of this strategy, we first characterized the gene-specific RNAi induced by a large subset (258 siRNAs, 129 genes) of the entire siRNA library used in this study (similar to 800 siRNAs, similar to 400 genes). We next demonstrated that multiplexed siRNAs could silence at least six genes to the same degree as when the genes were targeted individually. The entire library was then used in a screen in which randomly multiplexed siRNAs were assayed for their affect on cell viability. Using this strategy, several gene targets that influenced the viability of a breast cancer cell line were identified. This study suggests that the screening of randomly multiplexed siRNAs may provide an important avenue towards the identification of candidate gene targets for downstream functional analyses and may also be useful for the rapid identification of positive controls for use in novel assay systems. This approach is likely to be especially applicable where assay costs or platform limitations are prohibitive. C1 NCI, Gene Silencing Sect, Off Sci & Technol Partnership, OD,CCR,NIH, Bethesda, MD 20892 USA. NCI Frederick, Mol Target Dev Program, CCR, NIH, Frederick, MD USA. NCI, Genom & Bioinformat Grp, Mol Pharmacol Lab, NIH, Bethesda, MD 20892 USA. NCI, CCR, Antibody & Prot Purif Unit, NIH, Bethesda, MD 20892 USA. Qiagen Inc, Germantown, MD USA. RP Caplen, NJ (reprint author), NCI, Gene Silencing Sect, Off Sci & Technol Partnership, OD,CCR,NIH, Bethesda, MD 20892 USA. EM ncaplen@mail.nih.gov RI Caplen, Natasha/H-2768-2016 OI Caplen, Natasha/0000-0002-0001-9460 FU Intramural NIH HHS NR 34 TC 15 Z9 15 U1 0 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2007 VL 35 IS 8 AR e57 DI 10.1093/nar/gkm141 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178SE UT WOS:000247239600035 PM 17392344 ER PT J AU Matveeva, O Nechipurenko, Y Rossi, L Moore, B Saetrom, P Ogurtsov, AY Atkins, JF Shabalina, SA AF Matveeva, Olga Nechipurenko, Yury Rossi, Leo Moore, Barry Saetrom, Pal Ogurtsov, Aleksey Y. Atkins, John F. Shabalina, Svetlana A. TI Comparison of approaches for rational siRNA design leading to a new efficient and transparent method SO NUCLEIC ACIDS RESEARCH LA English DT Article ID ARTIFICIAL NEURAL-NETWORK; RNA INTERFERENCE; THERMODYNAMIC PARAMETERS; FUNCTIONAL SIRNAS; DANGLING ENDS; BASE-PAIRS; PREDICTION; SELECTION; SEQUENCES; EFFICACY AB Current literature describes several methods for the design of efficient siRNAs with 19 perfectly matched base pairs and 2 nt overhangs. Using four independent databases totaling 3336 experimentally verified siRNAs, we compared how well several of these methods predict siRNA cleavage efficiency. According to receiver operating characteristics (ROC) and correlation analyses, the best programs were BioPredsi, ThermoComposition and DSIR. We also studied individual parameters that significantly and consistently correlated with siRNA efficacy in different databases. As a result of this work we developed a new method which utilizes linear regression fitting with local duplex stability, nucleotide position-dependent preferences and total G/C content of siRNA duplexes as input parameters. The new method's discrimination ability of efficient and inefficient siRNAs is comparable with that of the best methods identified, but its parameters are more obviously related to the mechanisms of siRNA action in comparison with BioPredsi. This permits insight to the underlying physical features and relative importance of the parameters. The new method of predicting siRNA efficiency is faster than that of ThermoComposition because it does not employ time-consuming RNA secondary structure calculations and has much less parameters than DSIR. It is available as a web tool called 'siRNA scales'. C1 Univ Utah, Dept Human Genet, Salt Lake City, UT 84112 USA. Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 119991, Russia. Norwegian Univ Sci & Technol, Dept Comp & Informat Sci, NO-7491 Trondheim, Norway. Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. Univ Coll Cork, Biosci Inst, Cork, Ireland. RP Matveeva, O (reprint author), Univ Utah, Dept Human Genet, Salt Lake City, UT 84112 USA. EM omatveev@genetics.utah.edu RI Shabalina, Svetlana/N-8939-2013; OI Shabalina, Svetlana/0000-0003-2272-7473; Saetrom, Pal/0000-0001-8142-7441 FU Intramural NIH HHS; NHGRI NIH HHS [R43 HG003355, R43 HG003355-01] NR 37 TC 74 Z9 79 U1 0 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2007 VL 35 IS 8 AR e63 DI 10.1093/nar/gkm088 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178SE UT WOS:000247239600041 PM 17426130 ER PT J AU Barcenas, CH Wilkinson, AV Strom, SS Cao, Y Saunders, KC Mahabir, S Hernandez-Valero, MA Forman, MR Spitz, MR Bondy, ML AF Barcenas, Carlos H. Wilkinson, Anna V. Strom, Sara S. Cao, Yumei Saunders, Katherine C. Mahabir, Somdat Hernandez-Valero, Maria A. Forman, Michele R. Spitz, Margaret R. Bondy, Melissa L. TI Birthplace, years of residence in the United States, and obesity among Mexican-American adults SO OBESITY LA English DT Article DE weight gain; Hispanics; acculturation; public health ID BODY-MASS INDEX; SOCIOECONOMIC-STATUS; NATIONAL SAMPLE; ACCULTURATION; PREVALENCE; IMMIGRANTS; NATIVITY; SCALE; WOMEN AB Objective: To evaluate the association between birthplace (Mexico or U.S.) and obesity in men and women and to analyze the relationship between duration of U.S. residency and prevalence of obesity in Mexican immigrants. Research Methods and Procedures: We used cross-sectional data from 7503 adults of Mexican descent residing in Harris County, TX, to evaluate the relationships among BMI, birthplace, and years of residency in the U.S., controlling for demographic characteristics, physical activity level, and acculturation level. Results: U.S.-born adults had an increased risk (between 34% and 65%) of obesity compared with their Mexican-born counterparts. After controlling for recognized confounders and risk factors, this association was maintained in the highly acculturated only. Among highly acculturated obese U.S.-born men, 6% of the cases were attributable to the joint effect of birthplace and acculturation; in women, this proportion was 25%. Among Mexican-born women, there was an increasing trend in mean BMI with increasing duration of residency in the U.S.. Compared with immigrants who had lived in the U.S. for <5 years, Mexican-born women who had resided in the U.S. for >= 15 years had an adjusted BMI mean difference of 2.12 kg/m(2) (95% confidence interval, 1.53-2.72). Discussion: Mexican-born men and women have a lower risk of obesity than their U.S.-born counterparts, but length of U.S. residency among immigrants, especially in women, is directly associated with risk of obesity. Development of culturally specific interventions to prevent obesity in recent immigrants may have an important public health effect in this population. C1 Univ Texas, MD Anderson Canc Ctr, Dept Epidemiol, Unit 1340, Houston, TX 77030 USA. Med Coll Georgia, Dept Internal Med, Augusta, GA 30912 USA. Boston VA Healthcare Syst, Boston, MA USA. Univ Texas, MD Anderson Canc Ctr, Ctr Res Minor Hlth, Houston, TX 77030 USA. NCI, Lab Biosyst & Canc, Canc Res Ctr, Bethesda, MD 20892 USA. RP Bondy, ML (reprint author), Univ Texas, MD Anderson Canc Ctr, Dept Epidemiol, Unit 1340, 1155 Pressler Blvd, Houston, TX 77030 USA. EM mbondy@mdanderson.org RI Mahabir, Somdat/A-9788-2008 NR 32 TC 95 Z9 95 U1 2 U2 10 PU NORTH AMER ASSOC STUDY OBESITY PI SILVER SPRING PA 8630 FENTON ST, SUITE 918, SILVER SPRING, MD 20910 USA SN 1071-7323 J9 OBESITY JI Obesity PD APR PY 2007 VL 15 IS 4 BP 1043 EP 1052 DI 10.1038/oby.2007.537 PG 10 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA 157OI UT WOS:000245729300031 PM 17426341 ER PT J AU Zhang, J Robledo, C Troendle, J Barnhart, K Creinin, M Westhoff, C AF Zhang, Jun Robledo, Candace Troendle, James Barnhart, Kurt Creinin, Mitchell Westhoff, Carolyn TI Clinical indicators for success of misoprostol treatment after early pregnancy failure SO OBSTETRICS AND GYNECOLOGY LA English DT Meeting Abstract CT 55th Annual Clinical Meeting of the American-College-of-Obstetricians-and-Gynecologists CY MAY 05-09, 2007 CL San Diego, CA SP Amer Coll Obstetricians & Gynecologists C1 NICHHD, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD APR PY 2007 VL 109 IS 4 SU S BP 4S EP 4S PG 1 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA 172JW UT WOS:000246801600010 ER PT J AU Klebanoff, MA AF Klebanoff, Mark A. TI Gestational age - Not always what it seems SO OBSTETRICS AND GYNECOLOGY LA English DT Editorial Material C1 NICHHD, Div Epidemiol Stat & Prevent Res, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Klebanoff, MA (reprint author), NICHHD, Div Epidemiol Stat & Prevent Res, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. EM mk90h@nih.gov NR 6 TC 11 Z9 11 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD APR PY 2007 VL 109 IS 4 BP 798 EP 799 DI 10.1097/01.AOG.0000260114.88379.92 PG 2 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA 195AI UT WOS:000248384500002 PM 17400838 ER PT J AU Grobman, WA Lai, Y Landon, MB Spong, CY Leveno, KJ Rouse, DJ Varner, MW Moawad, AH Caritis, SN Harper, M Wapner, RJ Sorokin, Y Miodovnik, M Carpenter, M O'Sullivan, MJ Sibai, BM Langer, O Thorp, JM Ramin, SM Mercer, BM AF Grobman, William A. Lai, Yinglei Landon, Mark B. Spong, Catherine Y. Leveno, Kenneth J. Rouse, Dwight J. Varner, Michael W. Moawad, Atef H. Caritis, Steve N. Harper, Margaret Wapner, Ronald J. Sorokin, Yoram Miodovnik, Menachem Carpenter, Marshall O'Sullivan, Mary J. Sibai, Baha M. Langer, Oded Thorp, John M. Ramin, Susan M. Mercer, Brian M. CA NICHD MFMU TI Development of a nomogram for prediction of vaginal birth after cesarean delivery SO OBSTETRICS AND GYNECOLOGY LA English DT Article ID SCORING SYSTEM; SUCCESS; TRIAL; LABOR AB OBJECTIVE: To develop a model based on factors available at the first prenatal visit that predicts chance of successful vaginal birth after cesarean delivery (VBAC) for individual patients who undergo a trial of labor. METHODS: All women with one prior low transverse cesarean who underwent a trial of labor at term with a vertex singleton gestation were identified from a concurrently collected database of deliveries at 19 academic centers during a 4-year period. Using factors identifiable at the first prenatal visit, we analyzed different classification techniques in an effort to develop a meaningful prediction model for VBAC success. After development and cross-validation, this model was represented by a graphic nomogram. RESULTS: Seven-thousand six hundred sixty women were available for analysis. The prediction model is based on a multivariable logistic regression, including the variables of maternal age, body mass index, ethnicity, prior vaginal delivery, the occurrence of a VBAC, and a potentially recurrent indication for the cesarean delivery. After analyzing the model with cross-validation techniques, it was found to be both accurate and discriminating. CONCLUSION: A predictive nomogram, which incorporates six variables easily ascertainable at the first prenatal visit, has been developed that allows the determination of a patient-specific chance for successful VBAC for those women who undertake trial of labor. C1 Northwestern Univ, Dept Obstet & Gynecol, Chicago, IL 60611 USA. George Washington Univ, Ctr Biostat, Washington, DC USA. Ohio State Univ, Columbus, OH 43210 USA. NICHHD, Bethesda, MD 20892 USA. Univ Texas San Antonio, SW Med Ctr, Dallas, TX USA. Univ Alabama Birmingham, Birmingham, AL USA. Univ Chicago, Chicago, IL 60637 USA. Univ Utah, Salt Lake City, UT USA. Univ Pittsburgh, Pittsburgh, PA USA. Wake Forest Univ, Winston Salem, NC 27109 USA. Thomas Jefferson Univ, Philadelphia, PA 19107 USA. Wayne State Univ, Detroit, MI USA. Univ Cincinnati, Cincinnati, OH USA. Columbia Univ, New York, NY USA. Brown Univ, Providence, RI 02912 USA. Univ Miami, Miami, FL 33152 USA. Univ Tennessee, Memphis, TN USA. Univ Texas San Antonio, San Antonio, TX 78285 USA. Univ N Carolina, Chapel Hill, NC USA. Univ Texas San Antonio, Houston, TX USA. Case Western Reserve Univ, Cleveland, OH 44106 USA. RP Grobman, WA (reprint author), 333 East Super St,Suite 410, Chicago, IL 60611 USA. EM w-grobman@northwestern.edu RI Varner, Michael/K-9890-2013 OI caritis, steve/0000-0002-2169-0712; Varner, Michael/0000-0001-9455-3973 FU NICHD NIH HHS [HD21410, HD21414, HD27860, HD27861, HD27869, HD27905, HD27915, HD27917, HD34116, HD34122, HD34136, HD34208, HD34210, HD36801, HD40485, HD40500, HD40512, HD40544, HD40545, HD40560] NR 12 TC 98 Z9 103 U1 3 U2 12 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD APR PY 2007 VL 109 IS 4 BP 806 EP 812 DI 10.1097/01.AOG.0000259312.36053.02 PG 7 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA 195AI UT WOS:000248384500004 PM 17400840 ER PT J AU Berghella, V Owen, J MacPherson, C Yost, N Swain, M Dildy, GA Miodovnik, M Langer, O Sibai, B AF Berghella, Vincenzo Owen, John MacPherson, Cora Yost, Nicole Swain, Melissa Dildy, Gary A., III Miodovnik, Menachem Langer, Oded Sibai, Baha CA NICHD MFMU TI Natural history of cervical funneling in women at high risk for spontaneous preterm birth SO OBSTETRICS AND GYNECOLOGY LA English DT Article; Proceedings Paper CT 51st Annual Clinical Meeting of the American-College-of-Obstetricians-and-Gynecologists CY APR 26-30, 2003 CL NEW ORLEANS, LA SP Amer Coll Obstet & Gynecol ID TRANSVAGINAL SONOGRAPHY; PREMATURE DELIVERY; UTERINE CERVIX; LENGTH; ULTRASOUND; PREDICTOR; PATIENT AB OBJECTIVE: To estimate the natural history of funneling in the second trimester by transvaginal ultrasonograms and whether funneling increases the risk of spontaneous birth. METHODS: Secondary analysis of a blinded, multi-center observational study of women with at least one prior spontaneous preterm birth at 16.0-31.9 weeks who subsequently carried singleton gestations. Cervical length, funneling (membrane prolapse greater than or equal to 5 mm), funnel shape, and dynamic changes were recorded at 16-18 weeks, and then every 2 weeks until 23.9 weeks. Managing obstetricians were blinded to the ultrasonography results. The primary outcome was gestational age at delivery. RESULTS: Five hundred ninety scans were performed in 183 women, of which 60 (33%) had funneling observed on at least one of the serial evaluations. These 60 women delivered at an earlier gestational age at delivery than the 123 women without funneling (31.7 +/- 7.9 weeks compared with 36.9 +/- 4.4 weeks; P<.001). In the 60 women with funneling on at least one evaluation, the progression over time of internal os cervical anatomy from a "T" to a "V" to a "U" shape was associated with earlier gestational age at delivery, whereas resolution of "V" shape funnels was associated with term delivery. Women with a shortened cervical length less than 25 mm (n=60) had a similar gestational age at birth with or without funneling (30.6 +/- 8.0 weeks compared with 31.9 +/- 6.6 weeks; P=.59). After controlling for the shortest observed cervical length, largest funnel percent was not a significant independent risk factor. CONCLUSION: The natural history of second-trimester funneling has significant variability and a significant association with earlier gestational age at delivery. As an independent finding, funneling does not add appreciably to the risk of early gestational age at delivery associated with a shortened cervical length. C1 Thomas Jefferson Univ, Jefferson Med Coll, Dept Obstet & Gynecol, Div Maternal Fetal Med, Philadelphia, PA 19107 USA. Univ Alabama, Dept Obstet & Gynecol, Birmingham, AL 35294 USA. George Washington Univ, Ctr Biostat, Bethesda, MD USA. Emory Univ, Sch Med, Dept Obstet & Gynecol, Atlanta, GA USA. Wake Forest Univ, Bowman Gray Sch Med, Dept Obstet & Gynecol, Winston Salem, NC 27103 USA. Univ Utah, Dept Obstet & Gynecol, Salt Lake City, UT USA. Univ Cincinnati, Dept Obstet & Gynecol, Cincinnati, OH USA. Univ Texas, Dept Obstet & Gynecol, San Antonio, TX USA. Univ Tennessee, Dept Obstet & Gynecol, Memphis, TN 38103 USA. NICHHD, Bethesda, MD 20892 USA. RP Berghella, V (reprint author), Thomas Jefferson Univ, Jefferson Med Coll, Dept Obstet & Gynecol, Div Maternal Fetal Med, 834 Chestnut St,Suite 400, Philadelphia, PA 19107 USA. EM vincenzo.berghella@jefferson.edu FU NICHD NIH HHS [HD34210, HD19897, HD21414, HD27860, HD27861, HD27869, HD27905, HD34116, HD34135, HD36801, K24HD43314] NR 17 TC 26 Z9 31 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD APR PY 2007 VL 109 IS 4 BP 863 EP 869 DI 10.1097/01.AOG.0000258276.64005.ce PG 7 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA 195AI UT WOS:000248384500011 PM 17400847 ER PT J AU Alexander, JM Leveno, KJ Rouse, DJ Landon, MB Gilbert, S Spong, CY Varner, MW Moawad, AH Caritis, SN Harper, M Wapner, RJ Sorokin, Y Miodovnik, M O'Sullivan, MJ Sibai, BM Langer, O Gabbe, SG AF Alexander, James M. Leveno, Kenneth J. Rouse, Dwight J. Landon, Mark B. Gilbert, Sharon Spong, Catherine Y. Varner, Michael W. Moawad, Atef H. Caritis, Steve N. Harper, Margaret Wapner, Ronald J. Sorokin, Yoram Miodovnik, Menachem O'Sullivan, Mary J. Sibai, Baha M. Langer, Oded Gabbe, Steven G. CA NICHD MFMU TI Comparison of maternal and infant outcomes from primary cesarean delivery during the second compared with first stage of labor SO OBSTETRICS AND GYNECOLOGY LA English DT Article ID OPERATIVE DELIVERY; 2ND-STAGE; MORBIDITY; DURATION; PROGRESS; COHORT AB OBJECTIVE: To compare maternal and neonatal outcomes when primary cesarean delivery is performed in the second stage of labor compared with the first stage. METHODS: Between January 1, 1999, and December 31, 2000, a prospective observational study of primary cesarean deliveries was conducted at 13 university centers comprising the National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network. The primary outcomes of interest included a maternal composite (composed of at least one of the following: endometritis, intraoperative surgical complication, blood transfusion, or wound complication) and neonatal composite (which included at least one of the following: Apgar score of 3 or less at 5 minutes, neonatal death, neonatal intensive care unit admission, seizure, delivery room intubation in the absence of meconium, or fetal injury). RESULTS: A total of 11,981 cesarean deliveries were available for analysis: 9,265 were performed in the first stage and 2,716 in the second stage. Cesarean deliveries performed in the second stage were associated with longer operative times, epidural analgesia, chorioamnionitis, and higher birth weight (all P<.001). The maternal composite index was slightly increased in women undergoing cesarean delivery in the second stage of labor, primarily due to uterine atony, uterine incision extension, and incidental cystotomy. This difference was significant after multivariable analysis (odds ratio 1.21, 95% confidence interval 1.07-1.37). After multivariable analysis, the neonatal composite did not differ significantly between groups (odds ratio 0.96, 95% confidence interval 0.84-1.08). CONCLUSION: Cesarean delivery in the second stage of labor is associated with slightly increased maternal but not neonatal composite morbidity. C1 Univ Texas, SW Med Ctr, Dept Obstet & Gynecol, Dallas, TX 75235 USA. Univ Alabama, Birmingham, AL USA. Ohio State Univ, Columbus, OH 43210 USA. Univ Utah, Salt Lake City, UT USA. Univ Chicago, Chicago, IL 60637 USA. Univ Pittsburgh, Pittsburgh, PA USA. Wake Forest Univ, Winston Salem, NC 27109 USA. Thomas Jefferson Univ, Philadelphia, PA 19107 USA. Wayne State Univ, Detroit, MI USA. Univ Cincinnati, Cincinnati, OH USA. Univ Miami, Miami, FL 33152 USA. Univ Tennessee, Memphis, TN USA. Univ Texas, Hlth Sci Ctr, San Antonio, TX USA. Vanderbilt Univ, Nashville, TN USA. George Washington Univ, Ctr Biostat, Washington, DC USA. NICHHD, Bethesda, MD 20892 USA. RP Alexander, JM (reprint author), Univ Texas, SW Med Ctr, Dept Obstet & Gynecol, 5323 Harry Hines Blvd, Dallas, TX 75235 USA. EM james.alexander@utsouthwestern.edu RI Varner, Michael/K-9890-2013 OI caritis, steve/0000-0002-2169-0712; Varner, Michael/0000-0001-9455-3973 FU NICHD NIH HHS [HD27905, HD21410, HD21414, HD27860, HD27861, HD27869, HD27915, HD27917, HD34116, HD34122, HD34136, HD34208, HD34210, HD36801] NR 10 TC 44 Z9 44 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD APR PY 2007 VL 109 IS 4 BP 917 EP 921 DI 10.1097/01.AOG.0000257121.56126.fe PG 5 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA 195AI UT WOS:000248384500018 PM 17400854 ER PT J AU McSorley, MA Alberg, AJ Allen, DS Allen, NE Brinton, LA Dorgan, JF Pollak, M Tao, Y Helzlsouer, KJ AF McSorley, Meghan A. Alberg, Anthony J. Allen, Diane S. Allen, Naomi E. Brinton, Louise A. Dorgan, Joanne F. Pollak, Michael Tao, Yuzhen Helzlsouer, Kathy J. TI C-reactive protein concentrations and subsequent ovarian cancer risk SO OBSTETRICS AND GYNECOLOGY LA English DT Article; Proceedings Paper CT 96th Annual Meeting of the American-Association-for-Cancer-Research CY APR 16-20, 2005 CL Anaheim, CA SP Amer Assoc Canc Res ID CORONARY-HEART-DISEASE; COLORECTAL-CANCER; TUMOR PROMOTER; INFLAMMATION; PREVENTION; OVULATION; THERAPY; STATINS; MARKERS; WOMEN AB OBJECTIVE: To estimate the association between prediagnostic levels of C-reactive protein (CRP), a marker of chronic systemic inflammation, and subsequent development of ovarian cancer. METHODS: A multicenter, nested, case-control study was conducted, including women who developed ovarian cancer (case patients) and women who were cancer-free (controls) from the following cohorts: CLUE ("Give us a CLUE to cancer and heart disease") cohorts of Washington County, Maryland, the Columbia, Missouri Serum Bank, and the Island of Guernsey Prospective Study, United Kingdom. A total of 167 incident invasive epithelial ovarian cancer cases were identified and each matched to an average of two controls on cohort, age, race, menopausal status, time since last menstrual period, current hormone use, date of recruitment, and time of day of blood draw. Baseline serum samples were assayed for CRP concentrations, and estimates of risk associated with CRP levels were assessed using conditional logistic regression. RESULTS: Ovarian cancer risk was positively associated with increasing CRP concentrations. The risk of developing ovarian cancer among women in the highest third of the distribution of CRP compared with those in the lowest third was 1.72 (95% confidence interval 1.06-2.77), with evidence of an increasing risk with increasing concentration of CRP (P trend=0.02). Similar associations were observed using established clinical CRP cutpoints for heart disease risk (odds ratio 2.03, 95% confidence interval 1.20-3.47 for 3-10 mg/L compared with less than 1 mg/L, P trend=.008). If this association is causal, roughly 23% of ovarian cancer cases are attributed to chronic inflammation as indicated by elevated CRP concentrations. CONCLUSION: Higher circulating CRP concentrations in women who subsequently developed ovarian cancer support the hypothesized role of chronic inflammation in ovarian carcinogenesis. C1 Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA. Guys Hosp, Acad Oncol Unit, London SE1 9RT, England. Canc Res UK Epidemiol Unit, Oxford, England. NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. Fox Chase Canc Ctr, Philadelphia, PA 19111 USA. McGill Univ, Jewish Gen Hosp, Dept Oncol, Montreal, PQ H3T 1E2, Canada. Mercy Med Ctr, Womens Ctr Hlth & Med, Prevent & Res Ctr, Baltimore, MD 21202 USA. RP Helzlsouer, KJ (reprint author), Mercy Med Ctr, Womens Ctr Hlth & Med, Prevent & Res Ctr, 227 St Paul Pl, Baltimore, MD 21202 USA. EM khelzlso@mdmercy.com RI Pollak, Michael/G-9094-2011; Brinton, Louise/G-7486-2015 OI Pollak, Michael/0000-0003-3047-0604; Brinton, Louise/0000-0003-3853-8562 FU NCI NIH HHS [CA-86308, CA-97857] NR 27 TC 56 Z9 59 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD APR PY 2007 VL 109 IS 4 BP 933 EP 941 DI 10.1097/01.AOG.0000257126.68803.03 PG 9 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA 195AI UT WOS:000248384500021 PM 17400857 ER PT J AU Reddy, UM Wapner, RJ Rebar, RW Tasca, RJ AF Reddy, Uma M. Wapner, Ronald J. Rebar, Robert W. Tasca, Richard J. TI Infertility, assisted reproductive technology, and adverse pregnancy outcomes - Executive summary of a National Institute of Child Health and Human Development Workshop SO OBSTETRICS AND GYNECOLOGY LA English DT Editorial Material ID IN-VITRO FERTILIZATION; INTRACYTOPLASMIC SPERM INJECTION; BECKWITH-WIEDEMANN-SYNDROME; MONOZYGOTIC TWINNING RATE; MAJOR BIRTH-DEFECTS; CONGENITAL-MALFORMATIONS; EMBRYO-TRANSFER; INCREASED RISK; UNITED-STATES; BORN AB The National Institute of Child Health and Human Development held a workshop on September 12-13,2005, to summarize the risks for adverse pregnancy outcomes after assisted reproductive technology (ART), develop an approach to counseling couples regarding these risks, and establish a research agenda. Although the majority of ART children are normal, there are concerns about the increased risk for adverse pregnancy outcomes. More than 30% of ART pregnancies are twins or higher-order multiple gestations (triplets or greater) and more than one half of all ART neonates are the products of multifetal gestations, with an attendant increase in prematurity complications. Assisted reproductive technology singleton pregnancies also demonstrate increased rates of perinatal complications-small for gestational age infants, preterm delivery, and perinatal mortality-as well as maternal complications, such as preeclampsia, gestational diabetes, placenta previa, placental abruption, and cesarean delivery. Although it is not possible to separate ART-related risks from those secondary to the underlying reproductive pathology, the overall increased frequency of obstetric complications, including preterm birth and small for gestational age neonates, should be discussed with the couple. Significant gaps in knowledge were identified, and the basic science and clinical and epidemiologic research required to address these gaps is outlined. C1 NICHHD, Pregnancy & Perinatol Branch, NIH, Dept Hlth & Human Serv, Bethesda, MD USA. Columbia Univ, Dept Obstet & Gynecol, New York, NY USA. Amer Soc Reprod Med, Birmingham, AL USA. NICHHD, Reprod Sci Branch, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Reddy, UM (reprint author), 6100 Execut Blvd,Room 4B03F, Bethesda, MD 20892 USA. EM reddyu@mail.nih.gov NR 64 TC 143 Z9 151 U1 2 U2 11 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD APR PY 2007 VL 109 IS 4 BP 967 EP 977 DI 10.1097/01.AOG.0000259316.04136.30 PG 11 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA 195AI UT WOS:000248384500025 PM 17400861 ER PT J AU Smith, JA Albeitz, J Begley, C Caffery, B Nichols, K Schaumberg, D Schein, O AF Smith, Janine A. Albeitz, Julie Begley, Carolyn Caffery, Barbara Nichols, Kelly Schaumberg, Debra Schein, Oliver TI The epidemiology of dry eye disease: Report of the Epidemiology Subcommittee of the international Dry Eye WorkShop (2007) SO OCULAR SURFACE LA English DT Article; Proceedings Paper CT International Dry Eye Workshop CY 2007 CL Balitmore, DC DE DEWS; dry eye; Dry Eye WorkShop; epidemiology; risk factors; questionnaire ID PRIMARY SJOGRENS-SYNDROME; IN-SITU KERATOMILEUSIS; QUALITY-OF-LIFE; MEIBOMIAN GLAND SECRETIONS; SYSTEMIC-LUPUS-ERYTHEMATOSUS; BONE-MARROW-TRANSPLANTATION; FUNCTIONAL VISUAL-ACUITY; CONTACT-LENS WEAR; OCULAR SURFACE; RISK-FACTORS AB The report of the Epidemiology Subcommittee of the 2007 Dry Eye WorkShop summarizes current knowledge on the epidemiology of dry eye disease, providing prevalence and incidence data from various populations. It stresses the need to expand epidemiological studies to additional geographic regions, to incorporate multiple races and ethnicities in future studies, and to build a consensus on dry eye diagnostic criteria for epidemiological studies. Recommendations are made regarding several characteristics of dry eye questionnaires that might be suitable for use in epidemiological studies and randomized controlled clinical trials. Risk factors for dry eye and morbidity of the disease are identified, and the impact of dry eye disease on quality of life and visual function are outlined. Suggestions are made for further prospective research that would lead to improvement of both eye and general public health. C1 NEI, NIH, Bethesda, MD 20892 USA. RP Smith, JA (reprint author), NEI, NIH, 10 Ctr Dr,MSC 1204, Bethesda, MD 20892 USA. EM smithj@nei.nih.gov NR 118 TC 288 Z9 293 U1 6 U2 43 PU ETHIS COMMUNICATINS, INC PI NEW YORK PA 75 MAIDEN LANE, STE 408, NEW YORK, NY 10038 USA SN 1542-0124 J9 OCUL SURF JI Ocul. Surf. PD APR PY 2007 VL 5 IS 2 SI SI BP 93 EP 107 PG 15 WC Ophthalmology SC Ophthalmology GA 165PV UT WOS:000246318600004 ER PT J AU Haymond, M Anderson, B Barrera, P Brosnan, P Bush, C Green, T Holden, H Jeha, G Jones, M McGirk, S McKay, S Miller, D Schreiner, B Zarate, M Dahms, W Casey, T Cuttler, L Drotar, D Frieson, E Levers-Landis, C McGuigan, P Palmert, M Sundararajan, S Witherspoon, D Geffner, M Chang, N Dreimane, D Estrada, S Fabian, J Halvorson, M Hollen, B Kaufman, F Munoz, C Ward, A Yasuda, P Katz, L Allegretto, R Carchidi, C Kaplan, J Lassiter, C Magge, S Sababu, S Schwartzman, B Willi, S Arslanian, S Bacha, F Foster, S Hannon, T Kolenc, K Kriska, A Libman, I Marcus, M Rofey, D Scanlon, P Songer, T Venditti, E Weisbrod, V Goland, R Belle, J Cain, R Kringas, P Leibel, N Ng, D Ovalles, M Robbins, K Seidman, D Siegel-Czarkowski, L Nathan, D Laffel, L Angelescou, A Bissett, L Ciccarelli, C Corrales-Yauckoes, K Delahanty, L Goldman, V Higgins, L Hood, K Larkin, M Lee, M Levitsky, L Malloy, M McEachern, R Milaszewski, K Norman, D Nwosu, B Orkin, L Park-Bennett, S Richards, D Rodriguez-Ventura, A Sheehy, M Soyka, L Steiner, B Weinstock, R Bowerman, D Bratt, K Carusone, S Doolittle, S Duncan, K Franklin, R Hartsig, J Izquierdo, R Kearns, J Meyer, S Saletsky, R Trief, P Zeitler, P Bellon, K Cain, C Celona-Jacobs, N Gaskell, L Glazner, J Hanze, D Higgins, J Hoe, F Klingen-smith, G McCann, T Nadeau, K Van Dorsten, B Walders, N Copeland, K Brown, R Chadwick, J Macha, C Nordyke, A Olson, D Poulsen, T Pratt, L Preske, J Schanuel, J Sternlof, S Hale, D Amodei, N Favela-Prezas, R Gonzalez, D Haffner, S Hernandez, C Lozano, R Lynch, J Rivera, S Rodriguez, M Rupert, G Wauters, A White, N Tollefsen, S Arbelaez, A Carnes, S Dempsher, D Flomo, D Harris, M Jones, T Kociela, V Sadler, M Whelan, T Wolff, B Caprio, S Estrada, E Grey, M Guandalini, C Lavietes, S Rose, P Syme, A Tamborlane, W Hirst, K Coombs, L Drilea, S Edelstein, S Grover, N Lau, A Long, C Pyle, L Linder, B Marcovina, S Strylewicz, G Shepherd, J Sherman, M Mayer-Davis, E Thadikonda, P Wilfley, D Franklin, K O'Brien, D Patterson, J Tibbs, T Van Buren, D Welch, R Epstein, L Zhang, P Zeitler, P Epstein, L Grey, M Hirst, K Kaufman, F Tamborlane, W Wilfley, D AF Haymond, M. Anderson, B. Barrera, P. Brosnan, P. Bush, C. Green, T. Holden, H. Jeha, G. Jones, M. McGirk, S. McKay, S. Miller, D. Schreiner, B. Zarate, M. Dahms, W. Casey, T. Cuttler, L. Drotar, D. Frieson, E. Levers-Landis, C. McGuigan, P. Palmert, M. Sundararajan, S. Witherspoon, D. Geffner, M. Chang, N. Dreimane, D. Estrada, S. Fabian, J. Halvorson, M. Hollen, B. Kaufman, F. Munoz, C. Ward, A. Yasuda, P. Katz, L. Allegretto, R. Carchidi, C. Kaplan, J. Lassiter, C. Magge, S. Sababu, S. Schwartzman, B. Willi, S. Arslanian, S. Bacha, F. Foster, S. Hannon, T. Kolenc, K. Kriska, A. Libman, I. Marcus, M. Rofey, D. Scanlon, P. Songer, T. Venditti, E. Weisbrod, V. Goland, R. Belle, J. Cain, R. Kringas, P. Leibel, N. Ng, D. Ovalles, M. Robbins, K. Seidman, D. Siegel-Czarkowski, L. Nathan, D. Laffel, L. Angelescou, A. Bissett, L. Ciccarelli, C. Corrales-Yauckoes, K. Delahanty, L. Goldman, V. Higgins, L. Hood, K. Larkin, M. Lee, M. Levitsky, L. Malloy, M. McEachern, R. Milaszewski, K. Norman, D. Nwosu, B. Orkin, L. Park-Bennett, S. Richards, D. Rodriguez-Ventura, A. Sheehy, M. Soyka, L. Steiner, B. Weinstock, R. Bowerman, D. Bratt, K. Carusone, S. Doolittle, S. Duncan, K. Frnaklin, R. Hartsig, J. Izquierdo, R. Kearns, J. Meyer, S. Saletsky, R. Trief, P. Zeitler, P. Bellon, K. Cain, C. Celona-Jacobs, N. Gaskell, L. Glazner, J. Hanze, D. Higgins, J. Hoe, F. Klingen-smith, G. McCann, T. Nadeau, K. Van Dorsten, B. Walders, N. Copeland, K. Brown, R. Chadwick, J. Macha, C. Nordyke, A. Olson, D. Poulsen, T. Pratt, L. Preske, J. Schanuel, J. Sternlof, S. Hale, D. Amodei, N. Favela-Prezas, R. Gonzalez, D. Haffner, S. Hernandez, C. Lozano, R. Lynch, J. Rivera, S. Rodriguez, M. Rupert, G. Wauters, A. White, N. Tollefsen, S. Arbelaez, A. Carnes, S. Dempsher, D. Flomo, D. Harris, M. Jones, T. Kociela, V. Sadler, M. Whelan, T. Wolff, B. Caprio, S. Estrada, E. Grey, M. Guandalini, C. Lavietes, S. Rose, P. Syme, A. Tamborlane, W. Hirst, K. Coombs, L. Drilea, S. Edelstein, S. Grover, N. Lau, A. Long, C. Pyle, L. Linder, B. Marovina, S. Strylewicz, G. Shepherd, J. Sherman, M. Mayer-Davis, E. Thadikonda, P. Wilfley, D. Franklin, K. O'Brien, D. Patterson, J. Tibbs, T. Van Buren, D. Welch, R. Epstein, L. Zhang, P. Zeitler, P. Epstein, L. Grey, M. Hirst, K. Kaufman, F. Tamborlane, W. Wilfley, D. CA TODAY Study Grp TI Treatment options for type 2 diabetes in adolescents and youth: a study of the comparative efficacy of metformin alone or in combination with rosiglitazone or lifestyle intervention in adolescents with type 2 diabetes SO PEDIATRIC DIABETES LA English DT Review DE adolescents; lifestyle change; metformin; obesity; thiazolidinedione; type 2 diabetes ID BETA-CELL FUNCTION; HOMEOSTASIS MODEL ASSESSMENT; RANDOMIZED CONTROLLED-TRIAL; BECK DEPRESSION INVENTORY; GLUCOSE-TOLERANCE TEST; BODY-FAT DISTRIBUTION; PHYSICAL-ACTIVITY; OBESE CHILDREN; INSULIN-RESISTANCE; WEIGHT CHANGE AB Despite the increased prevalence of type 2 diabetes mellitus (T2DM) in the pediatric population, there is limited information about the relative effectiveness of treatment approaches. This article describes the rationale and design of a National Institutes of Health-sponsored multi-site, randomized, parallel group clinical trial designed to test the hypothesis that aggressive reduction in insulin resistance early in the course of T2DM is beneficial for prolongation of glycemic control, as well as improvement in associated abnormalities and risk factors. Specifically, the trial compares treatment with metformin with two alternate approaches, one pharmacologic (combining metformin treatment with rosiglitazone) and one combining metformin with an intensive lifestyle intervention program. The Treatment Options for Type 2 Diabetes in Adolescents and Youth (TODAY) study recruits 800 patients over a 4-yr period and follows them for a minimum of 2 yr and maximum of 6 yr. Patients are 10-17 yr of age, within 2 yr of diagnosis of diabetes at the time of randomization, lack evidence of autoimmunity, and have sustained C-peptide secretion. The primary outcome is time to loss of glycemic control, defined as a hemoglobin Alc >8% for 6 consecutive months. Secondary outcomes include the effect of the alternative treatments on insulin secretion and resistance, body composition, nutrition, physical activity and fitness, cardiovascular risk monitoring, microvascular complications, quality of life, depression, eating pathology, and resource utilization. TODAY is the first large-scale, systematic study of treatment effectiveness for T2DM in youth. When successfully completed, this study will provide critical new information regarding the natural history of T2DM in youth, the benefits of initiating early aggressive treatment in these patients, and the efficacy of delivering an intensive and sustained lifestyle intervention to children with T2DM. C1 Baylor Coll Med, Houston, TX 77030 USA. Case Western Reserve Univ, Cleveland, OH 44106 USA. Childrens Hosp Los Angeles, Los Angeles, CA 90027 USA. Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. George Washington Univ, Ctr Biostat, Washington, DC 20052 USA. NIDDK, Bethesda, MD 20892 USA. Univ Calif San Francisco, DEXA Reading Ctr, San Francisco, CA 94143 USA. Univ S Carolina, Diet Assessment Ctr, Columbia, SC 29208 USA. Washington Univ, Lifestyle Program Core, St Louis, MO 63130 USA. SUNY Buffalo, Buffalo, NY 14260 USA. Ctr Dis Control, Atlanta, GA 30333 USA. RP Haymond, M (reprint author), Baylor Coll Med, Houston, TX 77030 USA. OI Kriska, Andrea/0000-0002-3522-0869; Jeha, George/0000-0002-3531-5059 NR 100 TC 3 Z9 3 U1 1 U2 10 PU BLACKWELL PUBLISHING PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND SN 1399-543X J9 PEDIATR DIABETES JI Pediatr. Diabetes PD APR PY 2007 VL 8 IS 2 BP 74 EP 87 PG 14 WC Endocrinology & Metabolism; Pediatrics SC Endocrinology & Metabolism; Pediatrics GA 185SR UT WOS:000247731200005 ER EF