FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Ridge, JP Di Rosa, F Matzinger, P AF Ridge, JP Di Rosa, F Matzinger, P TI Conditioned Dendritic Cells turn on CD8 killers in the absence of CD4 help SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Meeting Abstract C1 NIAID, Cellular & Mol Immunol Lab, Ghost Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PY 1998 SU 2 MA B57 BP 43 EP 43 PG 1 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA 126TN UT WOS:000076309900124 ER PT J AU von Stebut, E Belkaid, Y Jakob, T Sacks, DL Udey, MC AF von Stebut, E Belkaid, Y Jakob, T Sacks, DL Udey, MC TI Induction of IL-12 release from Langerhans cell-like dendritic cells by Leishmania major amastigotes SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Meeting Abstract C1 NCI, Dermatol Branch, Bethesda, MD 20892 USA. NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. RI Jakob, Thilo/J-1621-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PY 1998 SU 2 MA E7 BP 57 EP 57 PG 1 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA 126TN UT WOS:000076309900176 ER PT J AU Zoeteweij, JP Golding, H Mostowski, H Blauvelt, A AF Zoeteweij, JP Golding, H Mostowski, H Blauvelt, A TI Cytokines regulate expression and function of the HIV coreceptor CXCR4 on human mature dendritic cells. SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Meeting Abstract C1 NCI, Dermatol Branch, Bethesda, MD 20892 USA. US FDA, Ctr Biol Evaluat & Res, Div Cell & Gene Therapy, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PY 1998 SU 2 MA E11 BP 58 EP 58 PG 1 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA 126TN UT WOS:000076309900180 ER PT J AU Anderson, CC Matzinger, P AF Anderson, CC Matzinger, P TI On the lifespan of activated APCs SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Meeting Abstract C1 NIAID, Ghost Lab, Cellular & Mol Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PY 1998 SU 2 MA F4 BP 66 EP 66 PG 1 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA 126TN UT WOS:000076309900212 ER PT J AU Hwang, ST Yamada, N Moore, AM Saeki, H AF Hwang, ST Yamada, N Moore, AM Saeki, H TI Cytokine-regulated expression of CCR7 in dendritic cells is associated with chemotactic response to Secondary Lymphoid-organ Chemokine SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Meeting Abstract C1 NCI, Dermatol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PY 1998 SU 2 MA G2 BP 75 EP 75 PG 1 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA 126TN UT WOS:000076309900245 ER PT J AU Su, H Messer, R Whitmire, W Fischer, E Portis, JC Caldwell, HD AF Su, H Messer, R Whitmire, W Fischer, E Portis, JC Caldwell, HD TI Vaccination against chlamydial genital tract infection following immunization with dendritic cells pulsed ex with non-viable chlamydiae SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Meeting Abstract C1 NIAID, Rocky Mt Labs, Lab Intracellular Parasites, NIH, Hamilton, MT 59840 USA. NIAID, Rocky Mt Labs, Microscopy Branch, NIH, Hamilton, MT 59840 USA. NIAID, Rocky Mt Labs, Lab Persistent Viral Dis, NIH, Hamilton, MT 59840 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PY 1998 SU 2 MA K8 BP 101 EP 101 PG 1 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA 126TN UT WOS:000076309900340 ER PT J AU Hiemenz, JW Walsh, TJ AF Hiemenz, JW Walsh, TJ TI Lipid formulations of amphotericin B SO JOURNAL OF LIPOSOME RESEARCH LA English DT Review DE amphotericin B; ABELCET (R); AmBisome (R); Amphocil (TM); mycosis ID INVASIVE FUNGAL-INFECTIONS; BONE-MARROW TRANSPLANT; COLONY-STIMULATING FACTOR; FAT EMULSION FORMULATION; CRITICALLY ILL PATIENTS; COLLOIDAL DISPERSION; PULMONARY ASPERGILLOSIS; DEOXYCHOLATE SUSPENSION; CRYPTOCOCCAL MENINGITIS; NEUTROPENIC PATIENTS C1 Duke Univ, Coll Med, Durham, NC 27706 USA. Florida Hosp, Bone Marrow Transplant Ctr, Orlando, FL USA. NCI, Immunocompromised Host Sect, Pediat Oncol Branch, Bethesda, MD 20892 USA. RP Hiemenz, JW (reprint author), Duke Univ, Coll Med, Durham, NC 27706 USA. EM john_hiemenz_md@mail.fhmis.net NR 120 TC 20 Z9 21 U1 0 U2 1 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 0898-2104 J9 J LIPOSOME RES JI J. Liposome Res. PY 1998 VL 8 IS 4 BP 443 EP 467 DI 10.3109/08982109809039931 PG 25 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy GA 146XC UT WOS:000077457300005 ER PT J AU Ito, Y Ma, Y AF Ito, Y Ma, Y TI Effects of coriolis force on countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID FLOW-THROUGH CENTRIFUGE; ROTATING SEALS AB During protein separation using the toroidal coil centrifuge, the partition efficiency was found to vary according to the direction of the elution through the coil. In most cases, eluting either the lighter phase toward the direction of rotation or the heavier phase in the opposite direction produced higher efficiencies. The cause of this unusual phenomenon may be attributed to the Coriolis force acting on the mobile phase in the rotating coil. Using a flow-through glass vial and colored mobile phases, the effects of the Coriolis force on moving droplets were observed under stroboscopic illumination. As expected, the path of the descending droplets (heavier phase) shifted against the direction of rotation whereas that of the ascending droplets (lighter phase) shifted toward the direction of rotation. The effect of this Coriolis flow on the partition efficiency was studied with a set of toroidal coils with various core diameters. Overall results indicated that the best partition efficiency may be obtained when the Coriolis flow is nearly parallel to the toroidal coil segment where the partition process is taking place. The hydrodynamic effects of the Coriolis force on the mobile phase are discussed. C1 NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20792 USA. RP Ito, Y (reprint author), NHLBI, Biophys Chem Lab, NIH, Bldg 10,Rm 7N322,10 Ctr Dr MSC 1676, Bethesda, MD 20792 USA. NR 8 TC 12 Z9 12 U1 0 U2 3 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 1 EP 17 DI 10.1080/10826079808001932 PG 17 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900002 ER PT J AU Qi, L Ma, Y Ito, Y Fales, HM AF Qi, L Ma, Y Ito, Y Fales, HM TI Isolation and purification of 3-oxo-Delta(5)-steroid isomerase from crude E-coli lysate by countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID FLOW-THROUGH CENTRIFUGE; ROTATING SEALS; SEPARATION; PROTEINS AB KSI (3-oxo-Delta(5)-steroid isomerase) was purified from crude E. coli lysate by countercurrent chromatography using a polymer phase system composed of polyethylene glycol 3350 and potassium phosphate (pH 7), each at 12.5% (w/w) in distilled water. Using the cross-axis coil planet centrifuge, a preparative scale separation was performed on 25 mL E. coli lysate containing ca 50 mg of KSI. About 40 mg of KSI was recovered at 98% purity. A small scale separation was performed on ca. 3 mg of N-15-labeled KSI using the toroidal coil centrifuge. The present method eliminates sample loss and denaturation caused by the solid support and yields pure proteins in both preparative and analytical separations. C1 NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Qi, L (reprint author), NHLBI, Biophys Chem Lab, NIH, Bldg 10,Rm 7N322,10 Ctr Dr MSC 1676, Bethesda, MD 20892 USA. NR 13 TC 8 Z9 8 U1 0 U2 2 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 83 EP 92 DI 10.1080/10826079808001937 PG 10 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900007 ER PT J AU Rasooly, A Ito, Y AF Rasooly, A Ito, Y TI Toroidal coil countercurrent chromatography separation of Staphylococcus aureus enterotoxin A in food SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID FLOW-THROUGH CENTRIFUGE; ROTATING SEALS AB Countercurrent Chromatography (CCC) has the potential to play a major role in food analysis because it permits analysis of crude and complex samples. CCC was evaluated for its ability to separate S. aureus enterotoxin A (SEA), which is a common cause of food poisoning. Mushrooms containing native-or heat-denatured SEA were separated by Toroidal Coil CCC and the fractions containing the toxin were analyzed by Western immunoblotting. Both native SEA, used to spike mushroom samples, and heat-denatured SEA, resulting from S. aureus contamination of canned mushrooms, were detectable using CCC. This method increases the level of sensitivity of Western immunoblotting by at least an order of magnitude by concentrating the sample. Our results suggest that CCC, in combination with a suitable detection method, is a potentially useful method for toxin analysis in food. C1 US FDA, Div Microbiol Studies, Washington, DC 20204 USA. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Rasooly, A (reprint author), US FDA, Div Microbiol Studies, Washington, DC 20204 USA. NR 13 TC 8 Z9 9 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 93 EP 102 DI 10.1080/10826079808001938 PG 10 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900008 ER PT J AU Matsuda, K Ma, Y Barghout, V Ito, Y Chatterjee, S AF Matsuda, K Ma, Y Barghout, V Ito, Y Chatterjee, S TI Isolation of less polar alkali-labile glycolipids of human brain by high-speed countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID PROTON MAGNETIC-RESONANCE; GALACTOSYL CERAMIDE; EPIDERMIS AB High-speed countercurrent chromatography (HSCCC), a liquid-liquid partition system, was used for the final purification of less polar alkali-labile glycolipids (ALGLs) of human brain. It has been reported that vertebrate brain contains ALGLs consisting of ester cerebroside and monoglucosyldiacylglycerol. ALGLs have alkali-labile ester bonds and are shown to be less polar than cerebroside. First, ALGLs (ALGL-I, II, III and IV) were extracted and isolated by repeated silica gel column chromatography. Then, the mixture of ALGLs was subjected to the HSCCC in which a solvent system, hexane/ethanol/water (5:4:1, by volume), was used with the lower phase mobile. ALGL-IV and -III were clearly separated. ALGL-IV, which was resolved as a single band on high performance thin-layer chromatography, was further separated into several components (ALGL-IVa, b, c, d and e). This is the first application of HSCCC for the separation of human brain ALGLs. The availability of purified ALGLs provides an opportunity to determine their structure, metabolic pathway, and function in relation to human health and disease. C1 Johns Hopkins Univ, Sch Med, Dept Pediat, Lipid Res Atherosclerosis Unit, Baltimore, MD 21287 USA. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Matsuda, K (reprint author), Johns Hopkins Univ, Sch Med, Dept Pediat, Lipid Res Atherosclerosis Unit, 600 N Wolfe St, Baltimore, MD 21287 USA. NR 16 TC 3 Z9 3 U1 0 U2 1 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 103 EP 110 DI 10.1080/10826079808001939 PG 8 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900009 ER PT J AU Shinomiya, K Kabasawa, Y Ito, Y AF Shinomiya, K Kabasawa, Y Ito, Y TI Protein separation by cross-axis coil planet centrifuge with two different types of coiled columns SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID PREPARATIVE COUNTERCURRENT CHROMATOGRAPHY; ROTARY SEALS; EFFICIENCY; APPARATUS; PHASE AB Proteins were separated using a cross-axis coil planet centrifuge equipped with two different types of coiled columns, i.e., toroidal and eccentric coil assemblies. An aqueous-aqueous polymer phase system composed of 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate was used for the separation of cytochrome C, myoglobin and ovalbumin. The toroidal coil assemblies with 1 mm ID tubing yielded the partition efficiency of 170 theoretical plates at the myoglobin peak. A slightly higher efficiency was obtained from the eccentric coil assemblies with the same diameter. The highest partition efficiency of 450 theoretical plates was attained from the eccentric coil assemblies with 0.85 mm ID and 64 mL capacity. C1 Nihon Univ, Coll Pharm, Chiba 274, Japan. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Shinomiya, K (reprint author), Nihon Univ, Coll Pharm, 7-7-1 Narashinodai, Chiba 274, Japan. NR 9 TC 17 Z9 17 U1 0 U2 1 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 111 EP 120 DI 10.1080/10826079808001940 PG 10 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900010 ER PT J AU Shibusawa, Y Yamaguchi, M Ito, Y AF Shibusawa, Y Yamaguchi, M Ito, Y TI Polyethylene glycol-potassium phosphate aqueous two-phase systems for countercurrent chromatography of proteins SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID COIL PLANET CENTRIFUGE; LIQUID PARTITION CHROMATOGRAPHY; STATIONARY-PHASE; HYDRODYNAMIC MECHANISM; SOLID SUPPORT; SEPARATION; RETENTION; APPARATUS; DESIGN; XLL AB Aqueous-aqueous polymer phase systems composed of polyethylene glycol 1000-potassium phosphate were used for the countercurrent chromatographic separation of proteins using the type XL cross-axis coil planet centrifuge. It was found that the peak resolution of proteins is highly dependant on the pH of the solvent system. The best separation of cytochrome c, myoglobin and human serum albumin was achieved with 16.0% PEG 1000-12.5% potassium phosphate buffer at pH 9.2. The resolution and separation time were improved by pH-peak-focusing countercurrent chromatography. C1 Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Analyt Chem, Tokyo 19203, Japan. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Shibusawa, Y (reprint author), Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Analyt Chem, 1432-1 Horinouchi, Tokyo 19203, Japan. NR 23 TC 9 Z9 11 U1 2 U2 6 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 121 EP 133 DI 10.1080/10826079808001941 PG 13 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900011 ER PT J AU Shinomiya, K Kabasawa, Y Ito, Y AF Shinomiya, K Kabasawa, Y Ito, Y TI Enantiomeric separation of commercial D,L-kynurenine with an aqueous two-phase solvent system by cross-axis coil planet centrifuge SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID 2-PHASE SYSTEM; COUNTERCURRENT AB Commercial D,L-kynurenine was resolved by high-speed countercurrent chromatography using a cross-axis coil planet centrifuge. The separation was performed with an aqueous-aqueous polymer phase system composed of 10% (w/w) polytheylene glycol 8000 and 5% (w/w) dibasic sodium phosphate containing 6% (w/w) bovine serum albumin as a chiral selector. The lower phosphate-rich mobile phase eluted the L-enantiomer first followed by the D-enantiomer. The peak resolution was 0.94 for 2.5 mg of the sample. The separation was completed within 3.5 h. C1 Nihon Univ, Coll Pharm, Chiba 274, Japan. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Shinomiya, K (reprint author), Nihon Univ, Coll Pharm, 7-7-1 Narashinodai, Chiba 274, Japan. NR 11 TC 18 Z9 20 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 135 EP 141 DI 10.1080/10826079808001942 PG 7 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900012 ER PT J AU Ikai, Y Oka, H Hayakawa, J Kawamura, N Harada, K Suzuki, M Nakazawa, H Ito, Y AF Ikai, Y Oka, H Hayakawa, J Kawamura, N Harada, K Suzuki, M Nakazawa, H Ito, Y TI Isolation of colistin A and B using high-speed countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY AB High-speed countercurrent chromatography was successfully applied to the isolation of colistin-A and colistin-B from a commercial colistin preparation. As the first step, TLC and HPLC analysis conditions for the colistin components were established. Using these techniques, a two-phase solvent system composed of n-butanol-0.04M aqueous trifluoroacetic acid (TFA) (1:1) was selected for high-speed CCC, where the concentration of TFA was the major factor in controlling the partition coefficients of the colistin components. Yields of colistin-A and colistin-B were 9 mg each from 20 mg of the commercial sample, and the purity of each component was over 90 %. Fast atom bombardment (FAB) mass spectrometry was utilized to confirm the nature of the isolated components. C1 Aichi Prefectural Inst Publ Hlth, Kita Ku, Nagoya, Aichi 462, Japan. Meijo Univ, Fac Pharm, Tempa Ku, Nagoya, Aichi 468, Japan. Hoshi Univ, Fac Pharmaceut Sci, Shinagawa Ku, Tokyo 142, Japan. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Ikai, Y (reprint author), Aichi Prefectural Inst Publ Hlth, Kita Ku, Nagoya, Aichi 462, Japan. NR 14 TC 18 Z9 18 U1 2 U2 2 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 143 EP 155 DI 10.1080/10826079808001943 PG 13 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900013 ER PT J AU Du, QZ Ke, CQ Ito, Y AF Du, QZ Ke, CQ Ito, Y TI Recycling high-speed countercurrent chromatography for separation of taxol and cephalomannine SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article AB A mixture of taxol and cephalomannine was subjected to recycling countercurrent chromatography (CCC) which allows the use of the same column for repetitive separation of the peak fractions to improve the peak resolution. When the total amount of 50 mg of the two components was recycled twice, peak resolution (R-s) increased from 0.7 to 1.27. The results also showed that R, value increases according to the formula: Rs-n = n(1/2)R(s-1) where n is the number of CCC runs, and Rs-1 and Rs-n, peak resolutions after the first and the nth run, respectively. C1 Chinese Acad Agr Sci, Tea Res Inst, Chinese Minist Agr, Key Lab Tea Chem Engn, Hangzhou 310008, Zhejiang, Peoples R China. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Du, QZ (reprint author), Chinese Acad Agr Sci, Tea Res Inst, Chinese Minist Agr, Key Lab Tea Chem Engn, Hangzhou 310008, Zhejiang, Peoples R China. NR 2 TC 16 Z9 17 U1 1 U2 6 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 157 EP 162 DI 10.1080/10826079808001944 PG 6 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900014 ER PT J AU Miao, P Cai, DC Xiang, BR An, DK Ito, Y AF Miao, P Cai, DC Xiang, BR An, DK Ito, Y TI Separation and purification of strychnine from crude extract of Strychnos nux-vomica L. by high-speed countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID ALKALOIDS AB High-speed countercurrent chromatography (CCC) was applied to the separation of strychnine and brucine from crude extract of Strychnos nux-vomica L. using a two-phase solvent system composed of chloroform/0.07 M sodium phosphate, 0.04 M citric buffer (pH 5.08) (1:1, v/v). Fractionated components were identified with authentic pure compounds on TLC and also analyzed by HPLC, LR and FTMS. The results showed that from 40 g of the seed powder, 48.2 mg of strychnine was purified at 99.9% purity (83.3% recovery) while 18.1 mg of brucine fraction was obtained after rechromatographed by preparative TLC improving the purity to 91.2%. C1 China Pharmaceut Univ, Instrument Ctr, Nanjing 210009, Peoples R China. PLA Navy, Res Ctr Pharm, Shanghai 200083, Peoples R China. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Miao, P (reprint author), China Pharmaceut Univ, Instrument Ctr, Nanjing 210009, Peoples R China. NR 9 TC 9 Z9 11 U1 0 U2 3 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 163 EP 170 DI 10.1080/10826079808001945 PG 8 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900015 ER PT J AU Ma, Y Qi, L Gnabre, JN Huang, RCC Chou, FE Ito, Y AF Ma, Y Qi, L Gnabre, JN Huang, RCC Chou, FE Ito, Y TI Purification of anti-HIV lignans from Larrea tridentata by pH-zone-refining countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article AB Anti-HIV lignans were purified from extract of Larrea tridentata by high-speed countercurrent chromatography (CCC) using pH-zone-refining CCC. When a column filled with methyl t-butyl ether, containing trifluoroacetic acid at 25 mM, was eluted with aqueous NaOH, 10 to 20 g of the crude extract was separated into NDGA (nordihydroguaiaretic acid) and its monomethyl esters rectangular peaks associated with their specific pH (pH zones). The method was also successfully applied to synthetic lignans, resulting in resolution of NDGA and its mono and dimethyl esters. C1 NHLBI, Lab Biomed Chem, NIH, Bethesda, MD 20892 USA. Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA. Pharma Tech Res Corp, Baltimore, MD 21212 USA. RP Ma, Y (reprint author), NHLBI, Lab Biomed Chem, NIH, Bldg 10,Rm 7N322,10 Ctr Dr MSC 1676, Bethesda, MD 20892 USA. NR 6 TC 6 Z9 6 U1 1 U2 4 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 171 EP 181 DI 10.1080/10826079808001946 PG 11 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900016 ER PT J AU Weisz, A Andrzejewski, D Highet, RJ Ito, Y AF Weisz, A Andrzejewski, D Highet, RJ Ito, Y TI Separation of a newly identified contaminant from commercial 4,5,6,7-tetrachlorofluorescein by ph-zone-refining countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID PURIFICATION AB A 5-g sample of commercial 4,5,6,7-tetrachlorofluorescein (TCF) was subjected to pH-zone-refining countercurrent chromatography to separate an unidentified contaminant present in all TCF batches obtained from four different suppliers. The separated contaminant (44 mg) was characterized by H-1- and C-13 nuclear magnetic resonance spectrometry and negative ion chemical ionization mass spectrometry as a new seven-membered lactone TCF isomer with the structure 11,12,13,14-tetrachloro-3,14b-dihydroxy-[2]-benzoxepino-[3,4,5-kl]-xanthene-10(14bH)-one. C1 US FDA, Off Cosmet & Colors, Washington, DC 20204 USA. US FDA, Off Sci & Anal & Support, Washington, DC 20204 USA. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Weisz, A (reprint author), US FDA, Off Cosmet & Colors, Washington, DC 20204 USA. NR 5 TC 3 Z9 3 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 183 EP 193 DI 10.1080/10826079808001947 PG 11 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900017 ER PT J AU Dudding, T Mekonnen, B Ito, Y Ziffer, H AF Dudding, T Mekonnen, B Ito, Y Ziffer, H TI Use of pH-zone-refining countercurrent chromatography to separate 2- and 6-nitro-4-chloro-3-methoxybenzoic acid SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID QUINOLINIC ACID; 4-CHLORO-3-HYDROXYANTHRANILATE AB A rapid, efficient separation of multigram quantities of 2- and 6-nitro-4-chloro-3-methoxybenzoic acid was achieved by pH-zone-refining CCC. C1 NIMH, Lab Neurotoxicol, NIH, Bethesda, MD 20892 USA. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. NIDDKD, Phys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Dudding, T (reprint author), NIMH, Lab Neurotoxicol, NIH, Bethesda, MD 20892 USA. NR 12 TC 2 Z9 2 U1 2 U2 3 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 195 EP 201 DI 10.1080/10826079808001948 PG 7 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900018 ER PT J AU Du, QZ Ke, CQ Ito, Y AF Du, QZ Ke, CQ Ito, Y TI Separation of epigallocatechin gallate and gallocatechin gallate using multiple instruments connected in series SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article AB A synthetic mixture of epigallocatechin gallate and gallocatechin gallate was separated using multiple countercurrent chromatographic instruments by connecting the separation columns in series. Peak resolution increased according to the formula Rs-n = n(1/2)R(s-1), where n is the number of columns connected; Rs-n and Rs-1, the peak resolution obtained from n columns and a single column, respectively. Various sample sizes and concentrations were applied to four columns connected in series. The results indicated that the sample loading capacity is increased 11 times that of the single instrument for the same peak resolution. C1 Chinese Minist Agr, Key Lab Tea Chem Engn, Tea Res Inst, Chinese Acad Agr Sci, Hangzhou 310008, Zhejiang, Peoples R China. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Du, QZ (reprint author), Chinese Minist Agr, Key Lab Tea Chem Engn, Tea Res Inst, Chinese Acad Agr Sci, Hangzhou 310008, Zhejiang, Peoples R China. NR 2 TC 17 Z9 17 U1 0 U2 1 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 203 EP 208 DI 10.1080/10826079808001949 PG 6 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900019 ER PT J AU Yang, FQ Zhang, TY Mo, BX Yang, LJ Gao, YQ Ito, Y AF Yang, FQ Zhang, TY Mo, BX Yang, LJ Gao, YQ Ito, Y TI Preparative separation and purification of kaempferol, isorhamnetin, and quercetin by high-speed countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID FLAVONOIDS AB High-speed countercurrent chromatography was used for the preparative separation and purification of kaempferol, isorhamnetin, and quercetin from leaf extracts of Ginkgo biloba L. and the commercial quercetin standard with a two-phase solvent system composed of chloroform-methanol-water (4:3:2, v/v/v). HPLC analyses of the CCC fractions revealed that all three main flavone aglycones were over 99% pure. Their chemical structures were identified by mass spectrometric analysis. C1 Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China. Shenzhen Res Ctr Agr Sci, Shenzhen 518040, Peoples R China. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Yang, FQ (reprint author), Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China. NR 7 TC 22 Z9 22 U1 1 U2 3 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 209 EP 216 DI 10.1080/10826079808001950 PG 8 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900020 ER PT J AU Mandava, NB Ito, Y Ma, Y AF Mandava, NB Ito, Y Ma, Y TI Separation of chlorflurenol-methyl and determination of octanol-water coefficient by countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article AB Using high-speed countercurrent chromatography (CCC), technical chlorflurenol-methyl was successfully separated into methyl 9-hydroxy-9-fluorenecarboxylate, methyl 2-chloro-9-hydroxy-9-fluorenecarboxylate, methyl 2,7-dicloro-9-hydroxy-9-fluorenecarboxylate. This method can be used for the determination of octanol-water coefficients (K-OW) for technical chlorflurenol-methyl and its separated components. The CCC can also be employed for the characterization and purification of the reaction products from synthesis of chlorflurenol-methyl. C1 Mandava Associates, Washington, DC 20006 USA. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Mandava, NB (reprint author), Mandava Associates, 1625 K St NW, Washington, DC 20006 USA. NR 4 TC 10 Z9 10 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 217 EP 229 DI 10.1080/10826079808001951 PG 13 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900021 ER PT J AU Kitazume, E Sato, N Ito, Y AF Kitazume, E Sato, N Ito, Y TI Concentration of heavy metals by high-speed countercurrent chromatography SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID RARE-EARTH ELEMENTS; SEPARATION AB Application of high-speed countercurrent chromatography (HSCCC) to the concentration of metal ions has been studied. The stationary phase, containing di(2-ethylhexyl) phosphoric acid (DEHPA) in n-heptane, was continuously fed into the column. The ppb level of metal ions in a 500 mL of the mobile phase was continuously concentrated into small volumes of the stationary phase retained in the column. Concentrated metal ions were eluted with nitric acid and determined by the emission intensity with a direct current plasma atomic emission spectrometer (DCP-AES). The recoveries of Ca, Cd, Mg, Mn, Pb and Zn ranged over 88% at a concentration of 10 ppb each in 500 mL of the sample solution. Versatility of the present method was further demonstrated in determination of trace metals in tap water and deionized water. C1 Iwate Univ, Fac Humanities & Social Sci, Morioka, Iwate 020, Japan. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Kitazume, E (reprint author), Iwate Univ, Fac Humanities & Social Sci, Morioka, Iwate 020, Japan. NR 11 TC 11 Z9 11 U1 0 U2 2 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 1-2 BP 251 EP 261 DI 10.1080/10826079808001953 PG 11 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA YY421 UT WOS:000072144900023 ER PT J AU Shinomiya, K Kabasawa, Y Ito, Y AF Shinomiya, K Kabasawa, Y Ito, Y TI Countercurrent chromatographic separation of proteins by cross-axis coil planet centrifuge: Choice of polymer phase systems and revolution speed SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article ID STATIONARY-PHASE; ROTARY SEALS; APPARATUS; DESIGN; ACCELERATION; EFFICIENCY; RETENTION AB Countercurrent chromatographic separation of proteins by the cross-axis coil planet centrifuge was maximized by selecting the suitable polymer phase system and revolution speed. Polymer phase systems composed of polyethylene glycol (PEG) 1000 and several inorganic salts were examined to determine partition coefficient values (K) for various proteins. The overall results indicated that the polymer phase system composed of 12.5% (w/w) PEG 1000 and 12.5% (w/w) dibasic potassium phosphate yielded suitable K values for most proteins except for cytochrome C and apo-transferrin which may be separable with a solvent system composed of 12.5% (w/w) PEG 1000 and 24% (w/w) potassium citrate. A series of experiments with the PEG 1000 potassium phosphate system under various revolution speeds revealed that the best separation was achieved at 850 rpm. The above optimized conditions may be applied to separations of other protein samples. C1 Nihon Univ, Coll Pharm, Funabashi, Chiba 274, Japan. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Shinomiya, K (reprint author), Nihon Univ, Coll Pharm, 7-7-1 Narashinodai, Funabashi, Chiba 274, Japan. NR 15 TC 8 Z9 9 U1 0 U2 4 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 11 BP 1727 EP 1736 DI 10.1080/10826079808001255 PG 10 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA ZW752 UT WOS:000074444400011 ER PT J AU Cao, XL Tian, Y Zhang, TY Shen, PN Zhu, LH Ito, Y AF Cao, XL Tian, Y Zhang, TY Shen, PN Zhu, LH Ito, Y TI HSCCC separation of a stilbene glucoside from Polygonum multiflorum SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article AB High-speed countercurrent chromatography (HSCCC) was applied to the separation and purification of 2,3,5,4'-tetrahydroxy stilbene-2-O-D-glucoside from dried roots (2.7g) of Polygonum multiflorum. The ethanol extract (240 mg) was first separated with the solvent system composed of ethyl acetate-ethanol-water at a volume ratio of 10:1:10 and the obtained fraction was repurified with the solvent system at the modified volume ratio of 50:1:50, The method produced stilbene glucoside (30mg) at a high purity of 96%. The chemical structure was identified by FAB-MS and (HNMR)-H-1. C1 Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China. Natl Engn Res Ctr Tradit Chinese med, Shanghai 200127, Peoples R China. NHLBI, Biophys Chem Lab, Bethesda, MD 20892 USA. RP Cao, XL (reprint author), Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China. NR 8 TC 6 Z9 9 U1 0 U2 2 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6076 J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PY 1998 VL 21 IS 18 BP 2897 EP 2904 DI 10.1080/10826079808003452 PG 8 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 130HP UT WOS:000076514500013 ER PT J AU Moro, S van Rhee, AM Sanders, LH Jacobson, KA AF Moro, S van Rhee, AM Sanders, LH Jacobson, KA TI Flavonoid derivatives as adenosine receptor antagonists: A comparison of the hypothetical receptor binding site based on a comparative molecular field analysis model SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID RAT-BRAIN; QSAR; EXPRESSION; INHIBITORS; PARAMETERS; CLONING; COMFA AB Flavonoid derivatives have been optimized as relatively rigid antagonists of adenosine receptors with particular selectivity for the A(3) receptor subtype. A quantitative study of the structure-activity relationships for binding of flavonoids to adenosine A(1), A(2A), and A(3) receptors has been conducted using comparative molecular field analysis (CoMFA). Correlation coefficients (cross-validated r(2)) of 0.605, 0.595, and 0.583 were obtained for the three subtypes, respectively. All three CoMFA models have the same steric and electrostatic contributions, implying similar requirements inside the binding cavity. Similarities were seen in the topology of steric and electrostatic regions with the A(1) and A(3) receptors, but not the A(2A). Substitutions on the phenyl ring at the C-2 position of the chromone moiety may be considered important for binding affinity at all adenosine receptors. In the A(3) model a region of favorable bulk interaction is located around the 2'-position of the phenyl ring. The presence of a C-6 substituent in the chromone moiety is well tolerated and increases the A(1)/A(3) selectivity. The CoMFA coefficient contour plots provide a self-consistent picture of the main chemical features responsible for the pK(i) variations and also result in predictions which agree with experimental values. C1 NIDDK, Mol Recognit Sect, NIH, LBC, Bethesda, MD 20892 USA. RP Jacobson, KA (reprint author), NIDDK, Mol Recognit Sect, NIH, LBC, Bldg 8A,Rm B1A-19, Bethesda, MD 20892 USA. EM jacobs@helix.nih.gov RI Moro, Stefano/A-2979-2012; Jacobson, Kenneth/A-1530-2009 OI Moro, Stefano/0000-0002-7514-3802; Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z99 DK999999, Z01 DK031117-20] NR 32 TC 48 Z9 48 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD JAN 1 PY 1998 VL 41 IS 1 BP 46 EP 52 DI 10.1021/jm970446z PG 7 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA YQ055 UT WOS:000071342900008 PM 9438021 ER PT J AU Kovbasnjuk, ON Szmulowicz, U Spring, KR AF Kovbasnjuk, ON Szmulowicz, U Spring, KR TI Regulation of the MDCK cell tight junction SO JOURNAL OF MEMBRANE BIOLOGY LA English DT Article DE glucose; protein kinase A; cytoskeleton; sodium permeability; transepithelial electrical resistance; phalloidin; cytochalasin D ID LATERAL INTERCELLULAR SPACES; GALL-BLADDER EPITHELIUM; CANINE KIDNEY-CELLS; INTESTINAL EPITHELIA; OCCLUDING JUNCTIONS; INTRACELLULAR CA2+; ION SELECTIVITY; 2 STRAINS; PERMEABILITY; CAMP AB The sodium flux across individual tight junctions (TJ) of low-resistance MDCK cell monolayers grown on glass coverslips was determined as a measure of paracellular permeability. Increases in perfusate glucose concentration from 5 to 25 mM decreased tight junction Na permeability. This permeability decrease was not specific as nonmetabolizable analogues of glucose caused similar diminutions in TJ Na permeability. Stimulation of protein kinase A increased TJ Na permeability, and inhibition of protein kinase A decreased TJ Na permeability. Transepithelial electrical resistance of monolayers grown on permeable supports did not change as predicted from the observed alterations in TJ Na permeability of monolayers grown on glass coverslips-. Fluorescent labeling of cell F-actin showed that increased F-actin in the perijunctional ring correlated with higher TJ Na permeability. Although a low dose of cytochalasin D did not change TJ Na permeability, it disrupted the cytoskeleton and blocked the decrease in TJ Na permeability caused by glucose. Cytochalasin D failed to block the effects of protein kinase A stimulation or inhibition on TJ Na permeability. We conclude that tight junction sodium permeability is regulated both by protein kinase A activity and by other processes involving the actin cytoskeleton. C1 NHLBI, Kidney & Electrolyte Metab Lab, NIH, Bethesda, MD 20892 USA. RP Kovbasnjuk, ON (reprint author), NHLBI, Kidney & Electrolyte Metab Lab, NIH, Bldg 10,Room 6N260, Bethesda, MD 20892 USA. NR 37 TC 31 Z9 31 U1 1 U2 3 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0022-2631 J9 J MEMBRANE BIOL JI J. Membr. Biol. PD JAN 1 PY 1998 VL 161 IS 1 BP 93 EP 104 DI 10.1007/s002329900317 PG 12 WC Biochemistry & Molecular Biology; Cell Biology; Physiology SC Biochemistry & Molecular Biology; Cell Biology; Physiology GA YP419 UT WOS:000071274900009 PM 9430624 ER PT J AU Neuwald, AF Koonin, EV AF Neuwald, AF Koonin, EV TI Ataxin-2, global regulators of bacterial gene expression, and spliceosomal snRNP proteins share a conserved domain SO JOURNAL OF MOLECULAR MEDICINE-JMM LA English DT Article ID SEQUENCE DATABASES; HOST FACTOR; IDENTIFICATION; TRANSLATION; EXPANSION; CLONING; REPEAT; TYPE-2; SM C1 NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,NIH,BETHESDA,MD 20894. NR 23 TC 33 Z9 33 U1 0 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0946-2716 J9 J MOL MED-JMM JI J. Mol. Med. PD JAN PY 1998 VL 76 IS 1 BP 3 EP 5 DI 10.1007/s109-1998-8098-0 PG 3 WC Genetics & Heredity; Medicine, Research & Experimental SC Genetics & Heredity; Research & Experimental Medicine GA YL383 UT WOS:A1998YL38300002 PM 9462862 ER PT J AU Pillemer, SR AF Pillemer, SR TI Introduction: Participants and overview SO JOURNAL OF MUSCULOSKELETAL PAIN LA English DT Editorial Material DE fibromyalgia; neuroendocrine; sleep ID FIBROMYALGIA C1 NIDR, NIH, Bethesda, MD 20892 USA. RP Pillemer, SR (reprint author), NIDR, NIH, Bldg 10,Room 1N113,10 Ctr Dr,MSC 1190, Bethesda, MD 20892 USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 USA SN 1058-2452 J9 J MUSCULOSKELET PAIN JI J. Musculoskelet. Pain PY 1998 VL 6 IS 3 BP 1 EP 4 DI 10.1300/J094v06n03_01 PG 4 WC Rehabilitation; Rheumatology SC Rehabilitation; Rheumatology GA 129AU UT WOS:000076441700002 ER PT J AU Pillemer, SR AF Pillemer, SR TI Chronic pain segment SO JOURNAL OF MUSCULOSKELETAL PAIN LA English DT Editorial Material DE fibromyalgia; pain ID HUMAN BRAIN MEASURES; SEX-DIFFERENCES; CLINICAL PAIN; FIBROMYALGIA; MANAGEMENT; ANALGESIA; GONADECTOMY; ARTHRITIS; GENDER C1 NIDR, NIH, Bethesda, MD 20892 USA. RP Pillemer, SR (reprint author), NIDR, NIH, Bldg 10,Room 1N113,10 Ctr Dr MSC 1190, Bethesda, MD 20892 USA. NR 22 TC 0 Z9 0 U1 3 U2 3 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 USA SN 1058-2452 J9 J MUSCULOSKELET PAIN JI J. Musculoskelet. Pain PY 1998 VL 6 IS 3 BP 5 EP 10 DI 10.1300/J094v06n03_02 PG 6 WC Rehabilitation; Rheumatology SC Rehabilitation; Rheumatology GA 129AU UT WOS:000076441700003 ER PT J AU Pillemer, SR AF Pillemer, SR TI Neuroendocrine segment SO JOURNAL OF MUSCULOSKELETAL PAIN LA English DT Editorial Material DE fibromyalgia; neuroendocrine; stress ID PITUITARY-ADRENAL AXIS; FUNCTIONAL HYPOTHALAMIC AMENORRHEA; PRIMARY FIBROMYALGIA SYNDROME; RELEASE; STRESS; HORMONE C1 NIDR, NIH, Bethesda, MD 20892 USA. RP Pillemer, SR (reprint author), NIDR, NIH, Bldg 10,Room 1N113,10 Ctr Dr MSC 1190, Bethesda, MD 20892 USA. NR 14 TC 0 Z9 0 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 USA SN 1058-2452 J9 J MUSCULOSKELET PAIN JI J. Musculoskelet. Pain PY 1998 VL 6 IS 3 BP 47 EP 50 DI 10.1300/J094v06n03_09 PG 4 WC Rehabilitation; Rheumatology SC Rehabilitation; Rheumatology GA 129AU UT WOS:000076441700010 ER PT J AU Chrousos, GP AF Chrousos, GP TI Neuroendocrine alterations associated with altered sleep, mood, and pain perception SO JOURNAL OF MUSCULOSKELETAL PAIN LA English DT Article ID PITUITARY-ADRENAL AXIS C1 NICHD, DEB, NIH, Bethesda, MD 20892 USA. RP Chrousos, GP (reprint author), NICHD, DEB, NIH, Bldg 10,Room 10N262,10 Ctr Dr, Bethesda, MD 20892 USA. NR 6 TC 4 Z9 4 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 USA SN 1058-2452 J9 J MUSCULOSKELET PAIN JI J. Musculoskelet. Pain PY 1998 VL 6 IS 3 BP 51 EP 55 DI 10.1300/J094v06n03_10 PG 5 WC Rehabilitation; Rheumatology SC Rehabilitation; Rheumatology GA 129AU UT WOS:000076441700011 ER PT J AU Goldstein, DS AF Goldstein, DS TI The sympathetic nervous and the adrenomedullary hormonal systems: Differential responses to stressors SO JOURNAL OF MUSCULOSKELETAL PAIN LA English DT Article ID PLASMA-CATECHOLAMINE; BEHAVIOR; PRESSURE; RATS C1 NINDS, Clin Neurosci Branch, NIH, Bethesda, MD 20892 USA. RP Goldstein, DS (reprint author), NINDS, Clin Neurosci Branch, NIH, 10-6N252,10 Ctr Dr MSC-1424, Bethesda, MD 20892 USA. NR 24 TC 1 Z9 1 U1 2 U2 2 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 USA SN 1058-2452 J9 J MUSCULOSKELET PAIN JI J. Musculoskelet. Pain PY 1998 VL 6 IS 3 BP 63 EP 68 DI 10.1300/J094v06n03_13 PG 6 WC Rehabilitation; Rheumatology SC Rehabilitation; Rheumatology GA 129AU UT WOS:000076441700014 ER PT J AU Pillemer, SR AF Pillemer, SR TI Sleep segment SO JOURNAL OF MUSCULOSKELETAL PAIN LA English DT Editorial Material DE fibromyalgia; sleep ID CIRCADIAN RHYTHMICITY; REM-SLEEP; FIBROMYALGIA; SYSTEM C1 NIDR, NIH, Bethesda, MD 20892 USA. RP Pillemer, SR (reprint author), NIDR, NIH, Bldg 10,Room 1N113,10 Ctr Dr MSC 1190, Bethesda, MD 20892 USA. NR 23 TC 0 Z9 0 U1 1 U2 1 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 USA SN 1058-2452 J9 J MUSCULOSKELET PAIN JI J. Musculoskelet. Pain PY 1998 VL 6 IS 3 BP 77 EP 85 DI 10.1300/J094v06n03_15 PG 9 WC Rehabilitation; Rheumatology SC Rehabilitation; Rheumatology GA 129AU UT WOS:000076441700016 ER PT J AU Pillemer, SR AF Pillemer, SR TI In this issue - The neuroscience and endocrinology of fibromyalgia - Concluding remarks SO JOURNAL OF MUSCULOSKELETAL PAIN LA English DT Article C1 NIDR, NIH, Bethesda, MD 20892 USA. RP Pillemer, SR (reprint author), NIDR, NIH, Bldg 10,Room 1N113,10 Ctr Dr MSC 1190, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 USA SN 1058-2452 J9 J MUSCULOSKELET PAIN JI J. Musculoskelet. Pain PY 1998 VL 6 IS 3 BP 103 EP 105 DI 10.1300/J094v06n03_18 PG 3 WC Rehabilitation; Rheumatology SC Rehabilitation; Rheumatology GA 129AU UT WOS:000076441700019 ER PT J AU Daly, JW AF Daly, JW TI Thirty years of discovering arthropod alkaloids in amphibian skin SO JOURNAL OF NATURAL PRODUCTS LA English DT Review ID POISON FROGS DENDROBATIDAE; DEPENDENT SODIUM-CHANNEL; PSEUDOPHRYNE-CORIACEA; AUSTRALIAN FROG; PUMILIOTOXIN-B; RAY-ANALYSIS; HISTRIONICOTOXINS; MYOBATRACHIDAE; RECEPTOR; BINDING AB Amphibian skin has provided a wide range of biologically active alkaloids. During the past 30 years, over 400 alkaloids of over 20 structural classes have been detected. These include the batrachotoxins, which are potent and selective activators of sodium channels, the histrionicotoxins, which are potent noncompetitive blockers of nicotinic receptor-gated channels, the pumiliotoxins and related allo-and homo-pumiliotoxins, which have myotonic and cardiotonic activity due to effects on sodium channels, and epibatidine, which has potent antinociceptive activity due to agonist activity at nicotinic receptors. Further classes of alkaloids from amphibian skin include pyrrolidines and piperidines, decahydroquinolines, pyrrolizidines, various indolizidines, quinolizidines, and tricyclic gephyrotoxins, pyrrolizidine oximes, pseudophrynamines, coccinellines, and cyclopentaquinolizidines. Most alkaloids of amphibian skin appear to be sequestered from dietary arthropods. The source of the batrachotoxins, histrionicotoxins, pumiliotoxins, epibatidine, and certain izidines are unknown. C1 NIDDKD, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. RP Daly, JW (reprint author), NIDDKD, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. NR 63 TC 207 Z9 214 U1 2 U2 29 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 J9 J NAT PROD JI J. Nat. Prod. PD JAN PY 1998 VL 61 IS 1 BP 162 EP 172 DI 10.1021/np970460e PG 11 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA YU540 UT WOS:000071728100038 PM 9461669 ER PT J AU Litvan, I Booth, V Wenning, GK Bartko, JJ Goetz, CG McKee, A Jankovic, J Jellinger, K Lai, EC Brandel, JP Verny, M Chaudhuri, KR Pearce, RKB Agid, Y AF Litvan, I Booth, V Wenning, GK Bartko, JJ Goetz, CG McKee, A Jankovic, J Jellinger, K Lai, EC Brandel, JP Verny, M Chaudhuri, KR Pearce, RKB Agid, Y TI Retrospective application of a set of clinical diagnostic criteria for the diagnosis of multiple system atrophy SO JOURNAL OF NEURAL TRANSMISSION LA English DT Article DE multiple system atrophy; clinical diagnosis; validity studies ID PROGRESSIVE SUPRANUCLEAR PALSY; RICHARDSON-OLSZEWSKI SYNDROME; PARKINSONS-DISEASE; OLIVOPONTOCEREBELLAR ATROPHY; STRIATONIGRAL DEGENERATION; GLUCOSE-METABOLISM; NATURAL-HISTORY; ROC ANALYSIS; FEATURES; ACCURACY AB We estimated the accuracy of a modified commonly used set of clinical diagnostic criteria for the diagnosis of multiple system atrophy (MSA) by retrospectively applying the criteria to the features recorded by six neurologists who had evaluated 105 autopsy-confirmed cases (16 MSA and 89 non-MSA disorders). Cases were abstracted from the records of the patients' first visit to an academic center: and were presented as clinical vignettes to six neurologists, each of whom recorded the main clinical features of the presented clinical vignette on a standardized form. Sensitivity and positive predictive values were chosen as validity outcome measures and were calculated by comparing the applied diagnostic criteria to the neuropathologic information. Of note, most MSA patients in this study (mainly those with Shy-Drager type) had not received levodopa therapy since the primary neurologists often had not perceived a need to administer this treatment. The validity of the retrospectively applied criteria for the diagnosis of possible MSA (sensitivity: median, 53%, range, 50-69%; positive predictive value: 30%, 28-39%) and probable MSA (sensitivity: 44%, 31-60%; positive predictive value: 68%, 54-80%) at the first visit was suboptimal. The best, still not perfect, accuracy for this set of diagnostic criteria was obtained when six out of eight features (sporadic adult onset, dysautonomia, parkinsonism, pyramidal signs, cerebellar signs, no levodopa response, no cognitive dysfunction, or no downward gaze supranuclear palsy) were present (median sensitivity, 59%; range, 50-75%; positive predictive value: 67%, 53-83%). This is the first study to validate criteria for the clinical diagnosis of MSA. Our data suggest that it is difficult to achieve an early and accurate clinical diagnosis of this disorder. The probability of correctly diagnosing MSA increases when at least six features of this modified set of criteria are present or when requiring the set for probable MSA. C1 NINCDS, Neuroepidemiol Branch, Bethesda, MD 20892 USA. NIMH, Div Epidemiol & Res Studies, Bethesda, MD 20892 USA. Neurol Inst, London, England. Rush Med Coll, Dept Neurol, Chicago, IL 60612 USA. Massachusetts Gen Hosp, Dept Neuropathol, Boston, MA 02114 USA. Baylor Coll Med, Dept Neurol, Houston, TX 77030 USA. Lainz Hosp, Ludwig Boltzmann Inst Clin Neurobiol, A-1130 Vienna, Austria. Federat Neurol, Paris, France. INSERM U289, Paris, France. Hop La Pitie Salpetriere, INSERM U360, Raymond Escourolle Neuropathol Lab, Paris, France. Inst Psychiat, Dept Neurol, London SE5 8AF, England. Neurol Inst, Parkinsons Dis Soc, Brain Tissue Bank, London, England. RP Litvan, I (reprint author), NINCDS, Med Neurol Branch, NIH, Fed Bldg,Room 714, Bethesda, MD 20892 USA. OI Litvan, Irene/0000-0002-3485-3445; Ray Chaudhuri, K/0000-0003-2815-0505 NR 32 TC 32 Z9 33 U1 0 U2 1 PU SPRINGER-VERLAG WIEN PI VIENNA PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA SN 0300-9564 J9 J NEURAL TRANSM JI J. Neural Transm. PY 1998 VL 105 IS 2-3 BP 217 EP 227 DI 10.1007/s007020050050 PG 11 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA ZN465 UT WOS:000073648600009 PM 9660099 ER PT J AU Murphy, DL Sims, K Eisenhofer, G Greenberg, BD George, T Berlin, F Zametkin, A Ernst, M Breakefield, XO AF Murphy, DL Sims, K Eisenhofer, G Greenberg, BD George, T Berlin, F Zametkin, A Ernst, M Breakefield, XO TI Are MAO-A deficiency states in the general population and in putative high-risk populations highly uncommon? SO JOURNAL OF NEURAL TRANSMISSION-SUPPLEMENT LA English DT Article; Proceedings Paper CT 6th Rappaport Symposium/7th Amine Oxidase Workshop CY JUN, 1996 CL SHAVEI ZION, ISRAEL SP Rappaport Family Inst Res Med Sci, Israel Inst Technol, Technion, Bruce Rappaport Fac Med, Israel Inst Technol, Int Union Biochem & Molec Biol, Hoffman La Roche Ltd, Switzerland, Synthelabo Rech, France, DeVries & Co Ltd, Israel, Teva Pharm Ind Ltd, Israel ID PLATELET MONOAMINE-OXIDASE; NORRIE DISEASE PATIENTS; CEREBROSPINAL-FLUID; B-GENES; PLASMA; METABOLISM; AMINE; NOREPINEPHRINE; INHIBITORS; DELETION AB Lack of monoamine oxidase A (MAO-A) due to either Xp chromosomal deletions or alterations in the coding sequence of the gene for this enzyme are associated with marked changes in monoamine metabolism and appear to be associated with variable cognitive deficits and behavioral changes in humans and in transgenic mice. In mice, some of the most marked behavioral changes are ameliorated by pharmacologically-induced reductions in serotonin synthesis during early development, raising the question of possible therapeutic interventions in humans with MAO deficiency states. At the present time, only one multi-generational family and a few other individuals with marked MAO-A deficiency states have been identified and studied in detail. Although MAO deficiency states associated with Xp chromosomal deletions were identified by distinct symptoms (including blindness in infancy) produced by the contiguous Norrie disease gene, the primarily behavioral phenotype of individuals with the MAO mutation is less obvious. This paper reports a sequential research design and preliminary results from screening several hundred volunteers in the general population and from putative high-risk groups for possible MAO deficiency states. These preliminary results suggest that marked MAO deficiency states are very rare. C1 NIMH, Clin Sci Lab, NIH, Ctr Clin, Bethesda, MD 20892 USA. NIMH, Child Psychiat Branch, NIH, Bethesda, MD 20892 USA. Massachusetts Gen Hosp, Mol Neurogenet Lab, Boston, MA 02114 USA. NINDS, Clin Neurosci Branch, NIH, Bethesda, MD 20892 USA. NIAAA, Clin Studies Lab, NIH, Bethesda, MD 20892 USA. Johns Hopkins Univ, Sch Med, Dept Psychiat, Baltimore, MD 21205 USA. RP Murphy, DL (reprint author), NIMH, Clin Sci Lab, NIH, Ctr Clin, 10-3D41,10 Ctr Dr MSC 1264, Bethesda, MD 20892 USA. NR 40 TC 4 Z9 4 U1 2 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0303-6995 J9 J NEURAL TRANSM-SUPP JI J. Neural Transm.-Suppl. PY 1998 IS 52 BP 29 EP 38 PG 10 WC Neurosciences SC Neurosciences & Neurology GA ZE895 UT WOS:000072842400007 ER PT J AU Murphy, DL Karoum, F Pickar, D Cohen, RM Lipper, S Mellow, AM Tariot, PN Sunderland, T AF Murphy, DL Karoum, F Pickar, D Cohen, RM Lipper, S Mellow, AM Tariot, PN Sunderland, T TI Differential trace amine alterations in individuals receiving acetylenic inhibitors of MAO-A (clorgyline) or MAO-B (selegiline and pargyline) SO JOURNAL OF NEURAL TRANSMISSION-SUPPLEMENT LA English DT Article; Proceedings Paper CT 6th Rappaport Symposium/7th Amine Oxidase Workshop CY JUN, 1996 CL SHAVEI ZION, ISRAEL SP Rappaport Family Inst Res Med Sci, Israel Inst Technol, Technion, Bruce Rappaport Fac Med, Israel Inst Technol, Int Union Biochem & Molec Biol, Hoffman La Roche Ltd, Switzerland, Synthelabo Rech, France, DeVries & Co Ltd, Israel, Teva Pharm Ind Ltd, Israel ID MONOAMINE-OXIDASE INHIBITORS; NORRIE DISEASE PATIENTS; CEREBROSPINAL-FLUID; L-DEPRENYL; ATYPICAL DEPRESSIVES; METABOLITES; DELETION; ANTIDEPRESSANTS; PLASMA; BRAIN AB Marked, dose-dependent elevations in the urinary excretion of phenylethylamine, para-tyramine, and meta-tyramine were observed in depressed patients treated for three or more weeks with 10, 30, or 60 mg/day of the partially-selective inhibitor of MAO-B, selegiline (l-deprenyl). In comparative studies with other, structurally similar acetylenic inhibitors of MAO, pargyline, an MAO-B > MAO-A inhibitor used in doses of 90 mg/day for three or more weeks, produced elevations in these trace amines which were similar to those found with the highest dose of selegiline studied. Clorgyline, a selective inhibitor of MAO-A used in doses of 30 mg/day for three or more weeks (a dose/time regimen previously reported to reduce urinary, plasma, and cerebrospinal fluid 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) > 80%, indicating a marked inhibitory effect on MAO-A in humans in vivo) produced negligible changes in trace amine excretion. In comparison to recent studies of individuals lacking the genes for MAO-A, MAO-B, or both MAO-A and MAO-B, the lack of change in trace amine excretion in individuals with a mutation affecting only MAO-A is in agreement with the observed lack of effect of clorgyline in the present study. Selegiline produced larger changes in trace amines - at least at the higher doses studied - than found in individuals lacking the gene for MAO-B, in agreement with other data suggesting a lesser selectivity for MAO-B inhibition when selegiline was given in doses higher than 10 mg/day. Overall, trace amine elevations in individuals receiving the highest dose of deprenyl or receiving pargyline were approximately three to five-fold lower than the elevations observed in individuals lacking the genes for both MAO-A and MAO-B, suggesting that these drug doses yield incomplete inhibition of MAO-A and MAO-B. C1 NIMH, Clin Sci Lab, NIH, Ctr Clin, Bethesda, MD 20892 USA. NIMH, Expt Therapeut Branch, Bethesda, MD 20892 USA. NIMH, Cerebral Metab Lab, Bethesda, MD 20892 USA. NIMH, St Elizabeths Hosp, Ctr Neurosci, Washington, DC 20032 USA. Duke Univ, Sch Med, Dept Psychiat, Durham, NC 27706 USA. Univ Michigan, Sch Med, Dept Psychiat, Ann Arbor, MI 48109 USA. Univ Rochester, Med Ctr, Dept Psychiat, Rochester, NY 14642 USA. RP Murphy, DL (reprint author), NIMH, Clin Sci Lab, NIH, Ctr Clin, 10-3D41,10 Ctr Dr MSC 1264, Bethesda, MD 20892 USA. NR 45 TC 12 Z9 12 U1 1 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0303-6995 J9 J NEURAL TRANSM-SUPP JI J. Neural Transm.-Suppl. PY 1998 IS 52 BP 39 EP 48 PG 10 WC Neurosciences SC Neurosciences & Neurology GA ZE895 UT WOS:000072842400008 ER PT J AU Finberg, JPM Wang, J Bankiewicz, K Harvey-White, J Kopin, IJ Goldstein, DS AF Finberg, JPM Wang, J Bankiewicz, K Harvey-White, J Kopin, IJ Goldstein, DS TI Increased striatal dopamine production from L-DOPA following selective inhibition of monoamine oxidase B by R(+)-N-propargyl-1-aminoindan (rasagiline) in the monkey SO JOURNAL OF NEURAL TRANSMISSION-SUPPLEMENT LA English DT Article; Proceedings Paper CT 6th Rappaport Symposium/7th Amine Oxidase Workshop CY JUN, 1996 CL SHAVEI ZION, ISRAEL SP Rappaport Family Inst Res Med Sci, Israel Inst Technol, Technion, Bruce Rappaport Fac Med, Israel Inst Technol, Int Union Biochem & Molec Biol, Hoffman La Roche Ltd, Switzerland, Synthelabo Rech, France, DeVries & Co Ltd, Israel, Teva Pharm Ind Ltd, Israel ID MAO-B; METABOLISM; BRAIN; RATS AB Striatal extracellular fluid concentrations of dopamine and metabolites in response to direct striatal administration of two L-DOPA boluses administered sequentially were determined in three rhesus monkeys during halothane anesthesia. Whereas in an initial microdialysis run, generation of dopamine was less following the second L-DOPA bolus than the first, in a subsequent run, in which the selective MAO-B inhibitor R(+)-N-propargyl-1-aminoindan (rasagiline) was administered systemically (0.2 mg/kg s.c.) between the two L-DOPA boluses, generation of dopamine was greater following the second bolus. C1 NINDS, Clin Neurosci Branch, NIH, Bethesda, MD 20892 USA. RP Finberg, JPM (reprint author), Technion Israel Inst Technol, Fac Med, Pharmacol Unit, POB 9649, Haifa, Israel. NR 15 TC 66 Z9 66 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0303-6995 J9 J NEURAL TRANSM-SUPP JI J. Neural Transm.-Suppl. PY 1998 IS 52 BP 279 EP 285 PG 7 WC Neurosciences SC Neurosciences & Neurology GA ZE895 UT WOS:000072842400031 ER PT J AU Brenneman, DE Hauser, J Davidson, A Gozes, I AF Brenneman, DE Hauser, J Davidson, A Gozes, I TI A femtomolar-acting neuroprotective peptide SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NICHD, Sect Dev & Mol Pharmacol, NIH, Bethesda, MD USA. Tel Aviv Univ, Dept Clin Biochem, Tel Aviv, Israel. NR 0 TC 0 Z9 0 U1 0 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 71 SU 1 BP S54 EP S54 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA ZV717 UT WOS:000074334000213 ER PT J AU Gozes, I Bassan, M Zamostiano, R Davidson, A Pinchasov, A Giladi, E Perl, O Bassan, H Blat, C Gibney, G Glazner, G Brenneman, DE AF Gozes, I Bassan, M Zamostiano, R Davidson, A Pinchasov, A Giladi, E Perl, O Bassan, H Blat, C Gibney, G Glazner, G Brenneman, DE TI Cloning of novel super active neuroprotective proteins SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NICHD, SDMP, LDN, NIH, Bethesda, MD USA. Tel Aviv Univ, Sackler Sch Med, IL-69978 Tel Aviv, Israel. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 71 SU 1 BP S53 EP S53 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA ZV717 UT WOS:000074334000212 ER PT J AU Pelsman, A Fernandez, G Gozes, I Brenneman, D Busciglio, J AF Pelsman, A Fernandez, G Gozes, I Brenneman, D Busciglio, J TI Activity-dependent neurotrophic factor peptides prevent degeneration of Down's syndrome neurons grown in culture. SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06030 USA. Tel Aviv Univ, Sackler Sch Med, Dept Clin Biochem, IL-69978 Tel Aviv, Israel. NICHHD, NIH, Bethesda, MD 20892 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 71 SU 1 BP S54 EP S54 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA ZV717 UT WOS:000074334000214 ER PT J AU Garcia, MC Ward, G Ma, YC Salem, N Kim, HY AF Garcia, MC Ward, G Ma, YC Salem, N Kim, HY TI Effect of docosahexaenoic acid on the synthesis of phosphatidylserine in rat brain microsomes and C6 glioma cells SO JOURNAL OF NEUROCHEMISTRY LA English DT Article DE phosphatidylserine; docosahexaenoic acid; serine base exchange; C6 glioma cells; brain; microsomes; electrospray mass spectrometry; n-3 deficiency ID ESSENTIAL FATTY-ACIDS; MASS-SPECTROMETRY; ETHANOLAMINE; MODULATION; SERINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHOGLYCERIDE; PHOSPHOLIPIDS; MEMBRANES AB Docosahexaenoic acid (22:6n-3) is the major polyunsaturated fatty acid (PUFA) in the CNS and accumulates particularly in phosphatidylserine (PS). We have investigated the effect of the 22:6n-3 compositional status on the synthesis of PS. The fatty acid composition of brain microsomes from offspring of rats artificially reared on an n-3-deficient diet showed a dramatic reduction of 22:6n-3 content (1.7 +/- 0.1%) when compared with control animals (15.0 +/- 0.2%). The decrease was accompanied by an increase in docosapentaenoic acid (22:5n-6) content, which replaced the 22:6n-3 phospholipids with 22:5n-6 molecular species, as demonstrated using HPLC/electrospray mass spectrometry. The n-3 deficiency did not affect the total amount of polyunsaturated phospholipids in brain microsomes; however, it was associated with a decrease in the total polyunsaturated PS content and with increased levels of 1-stearoyl-2-docosapentanoyl (18:0/22:5n-6) species, particularly in phosphatidylcholine. incorporation of [H-3]serine into PS in rat brain microsomes from n-3-deficient animals was slightly but significantly less than that of the control animals. Similarly, C6 glioma cells cultured for 24 h in 22:6n-3-supplemented media (10-40 mu M) showed a significant increase in the synthesis of [H-3]PS when compared with unsupplemented cells. Our data show that neuronal and glial PS synthesis is sensitive to changes in the docosahexaenoate levels of phospholipids and suggest that 22:6n-3 may be a modulator of PS synthesis. C1 NIAAA, Sect Mass Spectrometry, Lab Membrane Biochem & Biophys, NIH, Rockville, MD 20852 USA. RP Kim, HY (reprint author), NIAAA, Sect Mass Spectrometry, Lab Membrane Biochem & Biophys, NIH, Room 158,12420 Parklawn Dr, Rockville, MD 20852 USA. NR 29 TC 68 Z9 69 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PD JAN PY 1998 VL 70 IS 1 BP 24 EP 30 PG 7 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YN233 UT WOS:000071146400003 PM 9422343 ER PT J AU Beagles, KE Morrison, PF Heyes, MP AF Beagles, KE Morrison, PF Heyes, MP TI Quinolinic acid in vivo synthesis rates, extracellular concentrations, and intercompartmental distributions in normal and immune-activated brain as determined by multiple-isotope microdialysis SO JOURNAL OF NEUROCHEMISTRY LA English DT Article DE kynurenine pathway; in vivo microdialysis; endotoxin; blood; pharmacokinetics ID KYNURENINE PATHWAY METABOLISM; INSULIN-INDUCED HYPOGLYCEMIA; L-TRYPTOPHAN; NEUROLOGICAL DISEASE; QUANTITATIVE MICRODIALYSIS; DELAYED INCREASES; CEREBRAL-ISCHEMIA; BLOOD; MECHANISM; FLUID AB Quinolinic acid (QUIN) kills neurons by activation of NMDA receptors that are accessed via the extracellular fluid (ECF). In vivo microdialysis was employed to quantify the dynamics of ECF QUIN levels. [C-13(7)] QUIN was perfused through the probe for in vivo calibration to accurately quantify ECF QUIN concentrations, Osmotic pumps infused [H-2(3)]QUIN subcutaneously to quantify blood contributions to ECF and tissue levels, Local QUIN production rates and influx and efflux rates across the blood-brain barrier were calculated from the extraction fraction of [C-13(7)] QUIN, probe geometry, tissue diffusion coefficients, the extracellular volume fraction, and [H-2(3)]-QUIN/QUIN ratios in blood and dialysates. In normal brain, 85% of ECF QUIN levels (110 nM) originated from blood, whereas 59% of tissue homogenate QUIN (130 pmol/g) originated from local de novo synthesis. During systemic immune activation (intraperitoneal injection of endotoxin), brood QUIN levels increased (10.2-fold) and caused a rise in homogenate (10.8-fold) and ECF (18.5-fold) QUIN levels with an increase in the proportions of QUIN derived from blood. During CNS inflammation (local infusion of endotoxin), increases in brain homogenate (246-fold) and ECF (66-fold) QUIN levels occurred because of an increase in local synthesis rate (146-fold) and a reduction in efflux/influx ratio (by 53%). These results demonstrate that brain homogenate measures are a reflection of ECF concentrations, although there are quantitative differences in the values obtained. The mechanisms that maintain ECF QUIN levers at low Values cannot do so when there are large increases in local brain synthesis or when there are large elevations in blood QUIN concentrations. C1 NIMH, Lab Neurotoxicol, Bethesda, MD 20892 USA. NIH, Natl Ctr Res Resources, Bethesda, MD 20892 USA. RP Heyes, MP (reprint author), NIMH, Lab Neurotoxicol, Bldg 10,Rm 3D40,9000 Rockville Pike, Bethesda, MD 20892 USA. NR 42 TC 40 Z9 40 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PD JAN PY 1998 VL 70 IS 1 BP 281 EP 291 PG 11 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YN233 UT WOS:000071146400033 PM 9422373 ER PT J AU Rabin, O Chang, MCJ Grange, E Bell, J Rapoport, SI Deutsch, J Purdon, AD AF Rabin, O Chang, MCJ Grange, E Bell, J Rapoport, SI Deutsch, J Purdon, AD TI Selective acceleration of arachidonic acid reincorporation into brain membrane phospholipid following transient ischemia in awake gerbil SO JOURNAL OF NEUROCHEMISTRY LA English DT Article DE ischemia; fatty acid metabolism; phospholipid metabolism; acyl; CoA; brain; gerbil; reperfusion; recovery; arachidonic acid; palmitic acid ID NUCLEUS BASALIS MAGNOCELLULARIS; COENZYME-A SYNTHETASE; FREE FATTY-ACIDS; RAT-BRAIN; CEREBRAL-ISCHEMIA; ELECTROCONVULSIVE SHOCK; BILATERAL ISCHEMIA; GLOBAL-ISCHEMIA; NEURONAL DEATH; MOUSE-BRAIN AB Awake gerbils were subjected to 5 min of forebrain ischemia by clamping the carotid arteries for 5 min and then allowing recirculation. Radiolabeled arachidonic or palmitic acid was infused intravenously for 5 min at the start of recirculation, after which the brains were prepared for quantitative autoradiography or chemical analysis, Dilution of specific activity of the acyl-CoA pool was independently determined for these fatty acids in control gerbils and following 5 min of ischemia and 5 min of reperfusion, Using a quantitative method for measuring regional in vivo fatty acid incorporation into and turnover within brain phospholipids and determining unlabeled concentrations of acyl-CoAs following recirculation, it was shown that reperfusion after 5 min of ischemia was accompanied by a threefold increase compared with the control in the rate of reincorporation of unlabeled arachidonate that had been released during ischemia, whereas reincorporation of released palmitate was not different from the control. Selective and accelerated reincorporation of arachidonate into brain phospholipids shortly after ischemia may ameliorate specific deleterious effects of arachidonate and its metabolites on brain membranes. C1 NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. Hebrew Univ Jerusalem, Sch Pharm, Dept Pharmaceut Chem, IL-91120 Jerusalem, Israel. RP NIA, Neurosci Lab, NIH, Bldg 10,Room 6C103, Bethesda, MD 20892 USA. NR 46 TC 22 Z9 22 U1 0 U2 1 PU WILEY PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-3042 EI 1471-4159 J9 J NEUROCHEM JI J. Neurochem. PD JAN PY 1998 VL 70 IS 1 BP 325 EP 334 PG 10 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YN233 UT WOS:000071146400038 PM 9422378 ER PT J AU Chang, MCJ Purdon, AD Chikhale, EG Grange, E AF Chang, MCJ Purdon, AD Chikhale, EG Grange, E TI Effect of chronic lithium treatment on turnover of docosahexaenoic acid in brain phospholipids SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S74 EP S74 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200295 ER PT J AU Chen, Y McCarron, RM Spatz, M AF Chen, Y McCarron, RM Spatz, M TI Nitric oxide modulation of ET-1-induced Ca2+ mobilization in human cerebromicrovascular endothelial cell SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 USN, Med Res Inst, RMP, Bethesda, MD 20892 USA. NINDS, Stroke Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S71 EP S71 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200284 ER PT J AU Cohen, RI Hudson, LD Paras, P Gravel, M Braun, P Chandross, KJ AF Cohen, RI Hudson, LD Paras, P Gravel, M Braun, P Chandross, KJ TI CNP promoter driven expression of the recombinant bacterial fusion protein beta-galactosidase and neomycin phosphotransferase 2, GEO, allows selection of oligodendrocytes and Schwann cells from mixed cultures SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, LDN, NIH, Bethesda, MD 20892 USA. McGill Univ, Montreal, PQ H3G 1Y6, Canada. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S55 EP S55 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200218 ER PT J AU Contreras, MA Chang, MCJ Bell, JM Kirkby, D Rapoport, SI AF Contreras, MA Chang, MCJ Bell, JM Kirkby, D Rapoport, SI TI Reduced turnover of palmitic acid in brain phospholipids of pentobarbital anesthetized rats. SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, Epilepsy Res Branch, NIH, Bethesda, MD 20892 USA. NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S74 EP S74 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200296 ER PT J AU Eng, LF Lee, YL Kwan, H Brenner, M Messing, A AF Eng, LF Lee, YL Kwan, H Brenner, M Messing, A TI Astrocytes cultured from transgenic mice with the added GFAP gene contain Rosenthal fibers SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 VAPA Hlth Care Syst, Dept Pathol, Palo Alto, CA USA. Stanford Univ, Sch Med, Stanford, CA 94305 USA. NINDS, Stroke Branch, NIH, Bethesda, MD 20892 USA. Univ Wisconsin, Dept Pathobiol Sci, Madison, WI 53706 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S43 EP S43 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200171 ER PT J AU Espey, MG Kustova, Y Sei, Y Basile, AS AF Espey, MG Kustova, Y Sei, Y Basile, AS TI Glutamate is the principal excitotoxin in a murine model of AIDS dementia complex SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIDDK, Neurosci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S6 EP S6 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200022 ER PT J AU Farrer, RG Quarles, RH AF Farrer, RG Quarles, RH TI Expression and developmental regulation of O-acetylated gangliosides in oligodendrocyte lineage cells and their immunoreactivity with the A2B5 and JONES antibodies SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, Myelin & Brain Dev Sect, LMCN, NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S35 EP S35 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200138 ER PT J AU Harry, GJ AF Harry, GJ TI Cytokines response following chemical-induced hippocampal neurodegeneration. SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S60 EP S60 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200239 ER PT J AU Hoffer, BJ AF Hoffer, BJ TI Trophic factors: A therapeutic approach to Parkinson's disease SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIDA, IRP, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S39 EP S39 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200155 ER PT J AU Hudson, LD AF Hudson, LD TI Transcription factors that set the stage for neuronal and glial differentiation SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, Lab Dev Neurogenet, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S41 EP S41 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200165 ER PT J AU Lahtivirta, S Jones, CR Murphy, EJ Rapoport, SI AF Lahtivirta, S Jones, CR Murphy, EJ Rapoport, SI TI Developing a histochemical assay for detection of phospholipase A2 activity SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S74 EP S74 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200294 ER PT J AU Li, BS Miki, N Pant, HC AF Li, BS Miki, N Pant, HC TI Identification of chronic morphine-response element (CMRE) in the mouse Neurofilament (NF-L) gene promoter SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, Neurochem Lab, NIH, Bethesda, MD 20892 USA. Osaka Univ, Sch Med, Dept Pharmacol 1, Osaka 560, Japan. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S48 EP S48 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200193 ER PT J AU Luo, JJ Wallace, W Riccioni, T Ingram, D Roth, G Kusiak, JW AF Luo, JJ Wallace, W Riccioni, T Ingram, D Roth, G Kusiak, JW TI The role of amyloid precursor protein in Alzheimer's disease; Studies using adenovirus-mediated APP gene transfer SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIA, Mol Neurobiol Unit, Biol Chem Lab, NIH, Baltimore, MD 21224 USA. NIA, Cardiovasc Sci Lab, NIH, Baltimore, MD 21224 USA. NIA, Cellular & Mol Biol Lab, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 2 BP S52 EP S52 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA ZP236 UT WOS:000073731600206 ER PT J AU Maric, D Maric, I Barker, JL AF Maric, D Maric, I Barker, JL TI Endogenous GABA controls physiological properties of embryonic rat cortical neurons SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, Neurophysiol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S18 EP S18 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200073 ER PT J AU McKay, R AF McKay, R TI Stem cells, synapses and signals SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S41 EP S41 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200164 ER PT J AU Messing, A Head, MW Galles, K Galbreath, EJ Goldman, JE Brenner, M AF Messing, A Head, MW Galles, K Galbreath, EJ Goldman, JE Brenner, M TI Fatal encephalopathy with astrocyte inclusions in GFAP transgenic mice SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 Univ Wisconsin, Madison, WI 53706 USA. Columbia Univ, New York, NY 10032 USA. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S43 EP S43 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200172 ER PT J AU Morgan, L Li, W Pott, U Hudson, LD AF Morgan, L Li, W Pott, U Hudson, LD TI Differential distribution of rKr2, a CYS2HIS2 zinc finger protein, within the central nervous system SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, LDN, NIH, Bethesda, MD 20892 USA. NINDS, HHMI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S36 EP S36 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200143 ER PT J AU Murphy, EJ Oki, J Rapoport, SI Purdon, AD AF Murphy, EJ Oki, J Rapoport, SI Purdon, AD TI Rapid incorporation of [H-3]-hexadecanol from plasma into brain plasmalogens SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S73 EP S73 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200293 ER PT J AU Murphy, EJ Rapoport, SI AF Murphy, EJ Rapoport, SI TI Decreased ethanolamine plasmalogen levels in affected regions of Alzheimer disease brain SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S26 EP S26 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200103 ER PT J AU Nawashiro, H Messing, A Azzam, N Brenner, M AF Nawashiro, H Messing, A Azzam, N Brenner, M TI Mice lacking glial fibrillary acidic protein are hypersensitive to traumatic cerebrospinal injury SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, Stroke Branch, NIH, Bethesda, MD 20892 USA. Univ Wisconsin, Sch Vet Med, Madison, WI 53706 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S43 EP S43 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200170 ER PT J AU Ohno, M Quarles, RH AF Ohno, M Quarles, RH TI Phosphotyrosine signaling in immortalized Schwann cells SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, Myelin & Brain Dev Sect, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S14 EP S14 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200055 ER PT J AU Perichon, R Cunningham, SC Moser, AB Moser, HW Kelly, FJ Roth, GS AF Perichon, R Cunningham, SC Moser, AB Moser, HW Kelly, FJ Roth, GS TI Defective muscarinic cholinergic signal transduction in cell lines of patients with peroxisomal diseases (PD) SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIA, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA. Kennedy Krieger Inst, Baltimore, MD 21205 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S26 EP S26 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200104 ER PT J AU Qu, KB Lawrence, CE Martin, DL AF Qu, KB Lawrence, CE Martin, DL TI Structural fold of the cofactor binding site of human glutamate decarboxylase SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 New York State Dept Hlth, Wadsworth Ctr Labs & Res, Albany, NY 12201 USA. NIH, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S29 EP S29 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200114 ER PT J AU Shetty, HU Huang, W Schapiro, MB AF Shetty, HU Huang, W Schapiro, MB TI Myo-inositol is elevated in Down syndrome postmortem brain SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NCI, Neurosci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S66 EP S66 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200263 ER PT J AU Simpson, IA Appel, NM Hokari, M Oki, J Holman, GD Maher, F Koehler-Stec, EM Vannucci, SJ Smith, QR AF Simpson, IA Appel, NM Hokari, M Oki, J Holman, GD Maher, F Koehler-Stec, EM Vannucci, SJ Smith, QR TI Blood-brain barrier glucose transport: Effects of hyper- and hypoglycemia revisited. SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NIDDKD, NIH, Bethesda, MD 20892 USA. US FDA, NIH, Bethesda, MD 20892 USA. NIA, NIH, Bethesda, MD 20892 USA. Univ Bath, Dept Biochem, Bath BA2 7AY, Avon, England. Penn State Univ, Milton S Hershey Med Ctr, Dept Pediat, Hershey, PA 17033 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S68 EP S68 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200273 ER PT J AU Tanner, SL Rottkamp, CA Yim, SH Quarles, RH AF Tanner, SL Rottkamp, CA Yim, SH Quarles, RH TI MAP1B in developing neurons has an extracellular domain and binds to myelin-associated glycoprotein. SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 NINDS, Myelin & Brain Dev Sect, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S16 EP S16 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200062 ER PT J AU Vannucci, SJ Nehlig, A Koehler-Stec, EM Landshulz, WH Simpson, IA AF Vannucci, SJ Nehlig, A Koehler-Stec, EM Landshulz, WH Simpson, IA TI Developmental regulation of monocarboxylic acid and glucose transporter proteins in rat brain SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 Penn State Univ, Milton S Hershey Med Ctr, Dept Pediat, Hershey, PA 17033 USA. INSERM U398, Strasbourg, France. NIDDKD, NIH, Bethesda, MD 20892 USA. Univ Texas, SW Med Ctr, Dallas, TX 75235 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S68 EP S68 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200272 ER PT J AU Wilson, AF AF Wilson, AF TI Genetic strategies for complex inherited disorders SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 Natl Human Genome Res Inst, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1998 VL 70 SU 1 BP S3 EP S3 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA YY627 UT WOS:000072167200011 ER PT J AU Jezova, D Ochedalski, T Kiss, A Aguilera, G AF Jezova, D Ochedalski, T Kiss, A Aguilera, G TI Brain angiotensin II modulates sympathoadrenal and hypothalamic pituitary adrenocortical activation during stress SO JOURNAL OF NEUROENDOCRINOLOGY LA English DT Article DE AT1 receptors; stress; plasma catecholamines; CRH mRNA; CRH receptor mRNA; hypothalamic paraventricular nucleus ID CORTICOTROPIN-RELEASING FACTOR; MESSENGER-RIBONUCLEIC-ACID; PARAVENTRICULAR NUCLEUS; ACTH-SECRETION; ADRENAL AXIS; WATER-DEPRIVATION; SUPRAOPTIC NUCLEI; RAT HYPOTHALAMUS; RECEPTOR; SYSTEM AB Angiotensin II (Ang II) type-1 (AT(1)) receptors are present in areas of the brain controlling autonomic nervous activity and the hypothalamic-pituitary-adrenal (HPA) axis, including CRH cells in the hypothalamic paraventricular nucleus (PVN), To determine whether brain AT(1) receptors are involved in the activation of the HPA axis and sympathetic system during stress, we studied the effects of acute immobilization stress on plasma catecholamines, ACTH and corticosterone, and mRNA levels of CRH and CRH receptors (CRH-R) in the PVN in rats under central AT(1) receptor blockade by the selective antagonist, Losartan. While basal levels of epinephrine, norepinephrine and dopamine in plasma were unaffected 30 min after icy injection of Losartan (10 mu g), the increases after 5 and 20 min stress were blunted in Losartan treated rats (P < 0.05 for norepinephrine, and P < 0.01 for epinephrine and dopamine, vs controls), Basal or stress-stimulated plasma ACTH and corticosterone levels were unaffected by icy Losartan treatment, Using in situ hybridization studies, basal levels of CRH mRNA and CRH-R mRNA in the PVN were unchanged after icy Losartan, While Losartan had no effect on the increases in CRH-R mRNA levels 2 or 3 h after 1 h immobilization, it prevented the increases in CRH mRNA, The blunted plasma catecholamine responses after central AT(1) receptor blockade indicate that endogenous Ang II in the brain is required for sympathoadrenal activation during immobilization stress, While Ang II appears not to be involved in the acute secretory response of the HPA axis, it may play a role in regulating CRH expression in the PVN. C1 NICHD, Sect Endocrine Physiol, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA. Slovak Acad Sci, Inst Expt Endocrinol, Bratislava, Slovakia. RP Aguilera, G (reprint author), NICHD, Sect Endocrine Physiol, Dev Endocrinol Branch, NIH, Bldg 10,Room 10N262,10 Ctr Dr,MSC 1862, Bethesda, MD 20892 USA. NR 43 TC 96 Z9 100 U1 1 U2 2 PU BLACKWELL SCIENCE LTD PI OXFORD PA P O BOX 88, OSNEY MEAD, OXFORD OX2 0NE, OXON, ENGLAND SN 0953-8194 J9 J NEUROENDOCRINOL JI J. Neuroendocrinol. PD JAN PY 1998 VL 10 IS 1 BP 67 EP 72 DI 10.1046/j.1365-2826.1998.00182.x PG 6 WC Endocrinology & Metabolism; Neurosciences SC Endocrinology & Metabolism; Neurosciences & Neurology GA YZ467 UT WOS:000072257000009 PM 9510060 ER PT J AU Zipp, F Faber, E Sommer, N Muller, C Dichgans, J Krammer, PH Martin, R Weller, M AF Zipp, F Faber, E Sommer, N Muller, C Dichgans, J Krammer, PH Martin, R Weller, M TI CD95 expression and CD95-mediated apoptosis of T cells in multiple sclerosis - No differences from normal individuals and no relation to HLA-DR2 SO JOURNAL OF NEUROIMMUNOLOGY LA English DT Article DE autoimmune disease; multiple sclerosis; apoptosis; CD95; APO-1 antibody; peripheral blood mononuclear cells; HLA-DR2 ID SYSTEMIC LUPUS-ERYTHEMATOSUS; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; MYELIN BASIC-PROTEIN; FAS GENE-MUTATIONS; LYMPHOPROLIFERATIVE SYNDROME; IN-VITRO; BRAIN; SUSCEPTIBILITY; LYMPHOCYTES; CLONES AB CD95-mediated apoptosis is a potent endogenous pathway of T cell elimination that has been suggested to be altered in multiple sclerosis (MS). MS is associated with the HLA-DR2, Dw2, DQ6 HLA class II haplotype. We have previously reported that T cell lines hom HLA-DR2-positive individuals show enhanced production of tumor necrosis factor (TNF), a cytokine homologous to CD95 ligand, in response to specific antigen. Here we have studied CD95 expression and susceptibility to CD95-mediated apoptosis in peripheral blood mononuclear cells (PBMC) and activated T cells of 20 healthy individuals and 20 MS patients, half of whom were HLA-DR2-positive. MS patients did not differ from healthy individuals in either parameter. There was also no difference in CD95 expression or CD95-mediated apoptosis when MS patients and healthy individuals were grouped and compared according to HLA-DR status. These data reveal no differential regulation of PBMC/T cell apoptosis induced by CD95 receptor ligation in MS and show no impact of HLA-DR2 status on PBMC/T cell susceptibility to the same apoptotic stimulus. However, to assess the contribution of T cell apoptosis to the pathogenesis of MS further studies on other details of the complex system leading to T cell apoptosis are required. (C) 1998 Elsevier Science B.V. C1 Univ Tubingen, Dept Neurol, D-72076 Tubingen, Germany. Univ Tubingen, Dept Internal Med, D-7400 Tubingen, Germany. German Canc Res Ctr, D-6900 Heidelberg, Germany. NIND, Neuroimmunol Branch, NIH, Bethesda, MD USA. RP Zipp, F (reprint author), Univ Tubingen, Dept Neurol, Hoppe Seyler Str 3, D-72076 Tubingen, Germany. RI Zipp, Frauke/C-9968-2015 OI Zipp, Frauke/0000-0002-1231-1928 NR 31 TC 22 Z9 22 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-5728 J9 J NEUROIMMUNOL JI J. Neuroimmunol. PD JAN PY 1998 VL 81 IS 1-2 BP 168 EP 172 DI 10.1016/S0165-5728(97)00173-2 PG 5 WC Immunology; Neurosciences SC Immunology; Neurosciences & Neurology GA YZ472 UT WOS:000072257500019 PM 9521618 ER PT J AU Duhamel, JR Colby, CL Goldberg, ME AF Duhamel, JR Colby, CL Goldberg, ME TI Ventral intraparietal area of the macaque: Congruent visual and somatic response properties SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID POSTERIOR PARIETAL CORTEX; CORTICO-CORTICAL CONNECTIONS; MOUSE SUPERIOR COLLICULUS; OPTIC FLOW STIMULI; RHESUS-MONKEY; TEMPORAL CORTEX; FUNCTIONAL PROPERTIES; RECEPTIVE-FIELDS; AWAKE MONKEYS; MST NEURONS AB In a previous report, we described the visual response properties in the ventral intraparietal area (area VIP) of the awake macaque. Here we describe the somatosensory response properties in area VIP and the patterns of correspondence between the responses of single neurons to independently administered tactile and visual stimulation. VIP neurons responded to visual stimulation only or to visual and tactile stimulation. Of 218 neurons tested, 153 (70%) were bimodal in the sense that they responded to stimuli that were independently applied in either sensory modality. Unimodal visual and bimodal neurons were intermingled within the recording area and could not be distinguished on the basis of their visual response properties alone. Most of the cells with a tactile receptive field (RF) responded well to light touch or air puffs. The distribution of RF locations principally emphasized the head (85%), with approximately equivalent representations of the upper and lower face areas. The tactile and visual RFs were aligned in a congruent manner, with the intersection of the visual vertical and horizontal meridian having its tactile counterpart in the nose/mouth area. Small foveal visual RFs were paired with small tactile RFs on the muzzle, and peripheral Visual RFs were associated with tactile RFs on the side of the head or body. Most cells showed a strong sensitivity to moving stimuli, and the preferred directions of visual and tactile motion coincided In 85% of bimodal cells. In some cases, bimodal responses patterns were complementary: cells responding to motion in depth toward the monkey had ON responses. whereas cells responding to motion in depth away form the monkey had OFF responses. Other forms of bimodal response confluence included orientation selectivity, and ON, OFF, and ON/OFF response types. The large proportion of bimodal tactile and visual neurons with congruent response properties in area VIP indicates that there are important functional differences between area VIP and other dorsal stream areas involved in the analysis of motion. We suggest that VIP is involved in the construction of a multisensory, head-centered representation of near extrapersonal space. C1 CNRS, Coll France, Lab Physiol Percept & Act, F-75005 Paris, France. NEI, Sensorimotor Res Lab, Bethesda, MD 20892 USA. Univ Pittsburgh, Dept Neurosci, Pittsburgh, PA 15260 USA. Univ Pittsburgh, Ctr Neural Basis Cognit, Pittsburgh, PA 15260 USA. Georgetown Univ, Dept Neurol, Washington, DC 20057 USA. RP Duhamel, JR (reprint author), CNRS, Coll France, Lab Physiol Percept & Act, 11 Pl Marcelin Berthelot, F-75005 Paris, France. NR 65 TC 428 Z9 434 U1 1 U2 12 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD JAN PY 1998 VL 79 IS 1 BP 126 EP 136 PG 11 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA YR352 UT WOS:000071486800013 PM 9425183 ER PT J AU Degtyarenko, AM Simon, ES Norden-Krichmar, T Burke, RE AF Degtyarenko, AM Simon, ES Norden-Krichmar, T Burke, RE TI Modulation of oligosynaptic cutaneous and muscle afferent reflex pathways during fictive locomotion and scratching in the cat SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID FLEXOR DIGITORUM LONGUS; LUMBOSACRAL MOTONEURONS; WALKING CATS; EXCITATION; HINDLIMB; PHASE; INTERNEURONS; STIMULATION; TRANSMISSION; GENERATION AB Modulation of oligosynaptic cutaneous and muscle afferent reflex pathways during fictive locomotion and scratching in the cat. J. Neurophysiol. 79: 447-463, 1998. We have compared state-dependent transmission through oligosynaptic (minimally disynaptic) reflex pathways from low-threshold cutaneous and muscle afferents to some flexor and extensor lumbosacral motoneurons during fictive locomotion and scratching in decerebrate unanesthetized cats. As reported in earlier work, oligosynaptic cutaneous excitatory postsynaptic potentials (EPSPs) in flexor digitorum longus (FDL) and inhibitory postsynaptic potentials (IPSPs) in extensor digitorum (EDL) longus motoneurons were enhanced markedly during the early flexion phase of fictive locomotion. We show in this paper that, in contrast, these cutaneous reflex pathways were depressed markedly during ail phases of fictive scratching. On the other hand, disynaptic EPSPs produced by homonymous and synergist group I muscle afferents in flexor (tibialis anterior and EDL) motoneurons were present and strongly modulated during both fictive locomotion and scratching. During both actions, these disynaptic group I EPSPs appeared or exhibited the largest amplitude when the motoneuron membrane potential was most depolarized and the parent motor pool was active. There was an interesting exception to the simple pattern of coincident group I EPSP enhancement and motoneuron depolarization. During locomotion, disynaptic group I EPSPs in both FDL and flexor hallucis longus (FHL) motoneurons cells were facilitated during the extension phase, although FDL motoneurons were relatively hyperpolarized whereas FHL cells were depolarized The reverse situation was found during fictive scratching: group I EPSPs were facilitated in both FDL and FHL cells during the flexion phase when FDL motoneurons were depolarized and FHL cells were relatively hyperpolarized. These observations suggest that the disynaptic EPSPs in these two motor nuclei are produced by common interneurons. Reciprocal disynaptic inhibitory pathways from group Ia muscle afferents to antagonist motoneurons were also active and subject to phase-dependent modulation during both fictive locomotion and scratching. In all but one cell tested, reciprocal disynaptic group Ia IPSPs were largest during those phases in which the motoneuron membrane potential was relatively hyperpolarized and the parent motor pool was inactive. Oligosynaptic PSPs in motoneurons produced by stimulation of the mesencephalic locomotor region (MLR) were modulated strongly during fictive locomotion but were suppressed powerfully throughout fictive scratching. Large cord dorsum potentials generated by MLR stimuli also were suppressed markedly during fictive scratching. These results allow certain inferences about the organization of interneurons in the pathways examined. They also suggest that the central pattern generators that produce fictive locomotion and scratching are organized differently. C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. RP Burke, RE (reprint author), NINDS, Neural Control Lab, NIH, Bldg 49,Room 3A50, Bethesda, MD 20892 USA. NR 61 TC 54 Z9 54 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD JAN PY 1998 VL 79 IS 1 BP 447 EP 463 PG 17 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA YR352 UT WOS:000071486800043 PM 9425213 ER PT J AU Chub, N O'Donovan, MJ AF Chub, N O'Donovan, MJ TI Blockade and recovery of spontaneous rhythmic activity after application of neurotransmitter antagonists to spinal networks of the chick embryo SO JOURNAL OF NEUROSCIENCE LA English DT Article DE spinal plasticity; rhythmicity; embryonic networks; development; motoneurons; spontaneous neural activity ID ACTIVITY-DEPENDENT DEVELOPMENT; CENTRAL PATTERN GENERATOR; METHYL-D-ASPARTATE; XENOPUS EMBRYOS; MOTOR-ACTIVITY; NEURAL-NETWORK; SYNAPTIC DRIVE; CORD; NEURONS; MOTONEURONS AB We studied the regulation of spontaneous activity in the embryonic (day 10-11) chick spinal cord. After bath application of either an excitatory amino acid (AP-5 or CNQX) and a nicotinic cholinergic (DH beta E or mecamylamine) antagonist, or glycine and GABA receptor (bicuculline, 2-hydroxysaclofen, and strychnine) antagonists, spontaneous activity was blocked for a period (30-90 min) but then reappeared in the presence of the drugs. The efficacy of the antagonists was assessed by their continued ability to block spinal reflex pathways during the reappearance of spontaneous activity. Spontaneous activity ceased over the 4-5 hour monitoring period when both sets of antagonists were applied together. After application of glycine and GABA receptor antagonists, the frequency of occurrence of spontaneous episodes slowed and became highly variable. By contrast, during glutamatergic and nicotinic cholinergic blockade, the frequency of occurrence of spontaneous episodes initially slowed and then recovered to stabilize near the predrug level of activity. Whole-cell recordings made from ventral spinal neurons revealed that this recovery was accompanied by an increase in the amplitude of spontaneously occurring synaptic events. We also measured changes in the apparent equilibrium potential of the rhythmic, synaptic drive of ventral spinal neurons using voltage or discontinuous current clamp, After excitatory blockade, the apparent equilibrium potential of the rhythmic synaptic drive shifted similar to 10 mV more negative to approximately -30 mV. In the presence of bicuculline, the apparent equilibrium potential of the synaptic drive shifted toward the glutamate equilibrium potential. Considered with other evidence, these findings suggest that spontaneous rhythmic output is a general property of developing spinal networks, and that GABA and glycinergic networks alter their function to compensate for the blockade of excitatory transmission. C1 NINDS, Sect Dev Neurobiol, Neural Control Lab, NIH, Bethesda, MD 20892 USA. RP O'Donovan, MJ (reprint author), NINDS, Sect Dev Neurobiol, Neural Control Lab, NIH, Room 3A50,Bldg 49, Bethesda, MD 20892 USA. NR 48 TC 137 Z9 139 U1 0 U2 1 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD JAN 1 PY 1998 VL 18 IS 1 BP 294 EP 306 PG 13 WC Neurosciences SC Neurosciences & Neurology GA YP325 UT WOS:000071265600029 PM 9412508 ER PT J AU Moody, SL Wise, SP di Pellegrino, G Zipser, D AF Moody, SL Wise, SP di Pellegrino, G Zipser, D TI A model that accounts for activity in primate frontal cortex during a delayed matching-to-sample task SO JOURNAL OF NEUROSCIENCE LA English DT Article DE model; attractor; prefrontal; premotor; neural network; matching-re-sample; comparator ID POSTERIOR PARIETAL CORTEX; SHORT-TERM-MEMORY; MOTOR CORTEX; PREFRONTAL CORTEX; NEURONAL-ACTIVITY; VISUOMOTOR ACTIVITY; PREMOTOR CORTEX; NEURAL-NETWORK; ARM MOVEMENTS; MONKEY AB A fully recurrent neural network model was optimized to perform a spatial delayed matching-to-sample task (DMS). In DMS, a stimulus is presented at a sample location, and a match is reported when a subsequent stimulus appears at that location. Stimuli elsewhere are ignored. Computationally, a DMS system could consist of memory and comparison components. The model, although not constrained to do so, worked by using two corresponding classes of neurons in the hidden layer: storage and comparator units. Storage units form a dynamical system with one fixed point attractor for each sample location. Comparator units constitute a system receiving input from these storage units as well as from current input stimuli. Both unit types were tuned directionally. These two sources of information combine to create unique patterns of activity that determine whether a match has occurred. In networks with abundant hidden units, the storage and comparator functions were distributed so that individual units took pal? in both. We compared the model with single-neuron recordings from premotor (PM) and prefrontal (PF) cortex. As shown previously, many PM and PF neurons behaved like storage units. In addition, both regions contain neurons that behave like the comparator units of the model and appear to have dual functionality similar to that observed in the model units. No neuron in either area had properties identical to those of the match output neuron of the model. However, four PF neurons and one PM neuron resembled the output signal more closely than any of the hidden units of the model. C1 NIMH, Lab Syst Neurosci, Poolesville, MD 20837 USA. Univ Calif San Diego, Dept Cognit Sci, La Jolla, CA 92093 USA. RP Moody, SL (reprint author), NIMH, Lab Syst Neurosci, POB 608, Poolesville, MD 20837 USA. NR 34 TC 61 Z9 61 U1 0 U2 2 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD JAN 1 PY 1998 VL 18 IS 1 BP 399 EP 410 PG 12 WC Neurosciences SC Neurosciences & Neurology GA YP325 UT WOS:000071265600037 PM 9412516 ER PT J AU Villemagne, V Yuan, J Wong, DF Dannals, RF Hatzidimitriou, G Mathews, WB Ravert, HT Musachio, J McCann, UD Ricaurte, GA AF Villemagne, V Yuan, J Wong, DF Dannals, RF Hatzidimitriou, G Mathews, WB Ravert, HT Musachio, J McCann, UD Ricaurte, GA TI Brain dopamine neurotoxicity in baboons treated with doses of methamphetamine comparable to those recreationally abused by humans: Evidence from [C-11]WIN-35,428 positron emission tomography studies and direct in vitro determinations SO JOURNAL OF NEUROSCIENCE LA English DT Article DE methamphetamine; dopamine; neurotoxicity; PET; primates; WIN-35,428 ID COCAINE BINDING-SITES; TYROSINE-HYDROXYLASE ACTIVITY; NOREPINEPHRINE UPTAKE SITES; LESCH-NYHAN DISEASE; PARKINSONS-DISEASE; STRIATAL DOPAMINE; RAT-BRAIN; NERVE-TERMINALS; SINGLE INJECTION; C-11 WIN-35,428 AB The present study sought to determine whether doses of methamphetamine in the range of those used recreationally by humans produce brain dopamine (DA) neurotoxicity in baboons and to ascertain whether positron emission tomography (PET) imaging with the DA transporter (DAT) ligand [C-11]WIN-35,428 ([C-11]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)-tropane) could be used to detect methamphetamine-induced DAT loss in living primates. Baboons were treated with saline (n = 3) or one of three doses of methamphetamine [0.5 mg/kg (n = 2); 1 mg/kg (n = 2); and 2 mg/kg (n = 3)], each of which was given intramuscularly four times at 2 hr intervals. PET studies were performed before and 2-3 weeks after methamphetamine treatment. After the final PET studies, animals were killed for direct neurochemical determination of brain DA axonal markers. PET-derived binding potential values, used to index striatal DAT density, were significantly decreased after methamphetamine, with larger decreases occurring after higher methamphetamine doses. Reductions in striatal DAT documented by PET were associated with decreases in DA, dihydroxyphenylacetic acid, and specific [H-3]WIN-35,428 and [H-3]DTBZ binding determined in vitro. Decreases in DAT detected with PET were highly correlated with decreases in specific [H-3]WIN-35,428 binding determined in vitro in the caudate of the same animal (r = 0.77; p = 0.042). These results indicate that methamphetamine, at doses used by some humans, produces long-term reductions in brain DA axonal markers in baboons, and that it is possible to detect methamphetamine-induced DAT loss in living nonhuman primates by means of PET. C1 Johns Hopkins Med Inst, Dept Radiol, Div Nucl Med, Baltimore, MD 21205 USA. Johns Hopkins Med Inst, Dept Neurol, Baltimore, MD 21205 USA. NIMH, Unit Anxiety Disorders, Biol Psychiat Branch, Intramural Res Program, Bethesda, MD 20892 USA. RP Ricaurte, GA (reprint author), Johns Hopkins Med Inst, Dept Neurol, 5501 Bayview Dr,Room 5B71E, Baltimore, MD 21224 USA. FU NIDA NIH HHS [DA05707, DA06275, DA09482] NR 74 TC 162 Z9 162 U1 1 U2 3 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD JAN 1 PY 1998 VL 18 IS 1 BP 419 EP 427 PG 9 WC Neurosciences SC Neurosciences & Neurology GA YP325 UT WOS:000071265600039 PM 9412518 ER PT J AU Delporte, C Miller, G Kagami, H Lillibridge, CD O'Connell, BC Atkinson, JC Baum, BJ AF Delporte, C Miller, G Kagami, H Lillibridge, CD O'Connell, BC Atkinson, JC Baum, BJ TI Safety of salivary gland-administered replication-deficient recombinant adenovirus in rats SO JOURNAL OF ORAL PATHOLOGY & MEDICINE LA English DT Article DE adenovirus; aquaporin-1; gene transfer; safety; salivary gland ID GENE-THERAPY; NONHUMAN-PRIMATES; MEDIATED TRANSFER; CYSTIC-FIBROSIS; LUNG AB We have examined the safety of a replication-deficient recombinant adenovirus administered at a single, high dose intraductally to rat submandibular glands or systemically via the femoral vein. The virus used directed the synthesis of human aquaporin-1, a water channel protein, and is termed AdhAQP1. Comparisons were made 1 and 9 days post-infection with animals administered either a similar virus encoding no transgene or the viral suspension buffer. Animals were specifically not given anti-inflammatory drugs to impede the well-known immunopathologic response to recombinant adenoviral administration. Serum chemistries and hematological parameters were monitored. Rats were subjected to complete gross necropsy and selected tissues were evaluated by histopathology. Most clinical chemistry and hematology values were within normal ranges; however, evidence of inflammation (e.g., elevated lactic dehydrogenase, total leukocyte count) was seen. Gross pathology was normal, as was histopathology, excepting rare focal areas of necrosis. The results show that intrasalivary gland or intravenous AdhAQP1 administration leads to low levels of toxicity in rats. C1 NIDR, GTTB, NIH, Bethesda, MD 20892 USA. NIH, Vet Resources Program, Natl Ctr Res Resources, Bethesda, MD 20892 USA. RP Baum, BJ (reprint author), NIDR, GTTB, NIH, 10 Ctr Dr,MSC 1190,Bldg 10,Room 1N113, Bethesda, MD 20892 USA. RI Delporte, Christine/A-5733-2012; OI O'Connell, Brian/0000-0003-4529-7664 NR 18 TC 12 Z9 13 U1 0 U2 0 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0904-2512 J9 J ORAL PATHOL MED JI J. Oral Pathol. Med. PD JAN PY 1998 VL 27 IS 1 BP 34 EP 38 PG 5 WC Dentistry, Oral Surgery & Medicine; Pathology SC Dentistry, Oral Surgery & Medicine; Pathology GA YM530 UT WOS:000071074000008 PM 9466733 ER PT J AU Roberts, ISD Kunne, S DeCaestecker, M Bottinger, EP AF Roberts, ISD Kunne, S DeCaestecker, M Bottinger, EP TI Role of TGF beta in progressive renal fibrosis induced by acute accelerated hypertension in salt sensitive dahl rats SO JOURNAL OF PATHOLOGY LA English DT Meeting Abstract C1 NCI, Chemoprevent Lab, NIH, Bethesda, MD 20892 USA. Univ Manchester, Dept Pathol Sci, Manchester M13 9PL, Lancs, England. NR 0 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX PO19 1UD, ENGLAND SN 0022-3417 J9 J PATHOL JI J. Pathol. PY 1998 VL 184 SU S BP 12A EP 12A PG 1 WC Oncology; Pathology SC Oncology; Pathology GA YW337 UT WOS:000071924600049 ER PT J AU Weintraub, M Bhatia, KG Chandra, RS Magrath, IT Ladisch, S AF Weintraub, M Bhatia, KG Chandra, RS Magrath, IT Ladisch, S TI p53 expression in Langerhans cell histiocytosis SO JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY LA English DT Article DE Langerhans cell histiocytosis; p53; LCH cells; CD1a; cell proliferation ID ABNORMAL EXPRESSION; NUCLEAR ANTIGEN; TUMOR-ANTIGEN; PROTEIN; APOPTOSIS; CANCER; GENE; OVEREXPRESSION; MUTATIONS; CARCINOMA AB Purpose: Langerhans cell histiocytosis (LCH) is a disorder of unknown etiology involving the proliferation and accumulation of cells with the phenotype of a bone marrow-derived antigen-presenting cell of the skin, the Langerhans cell. We have studied p53 expresssion, an element in the control of cell proliferation, to determine whether it plays a role in the pathogenesis of LCH. Patients and Methods: LCH lesions from 10 patients with either localized (n = 5) or multisystem disease (n = 5) were studied. p53 protein expression was assessed by immunohistochemistry, and p53 gene mutation by the single strand conformation polymorphism (SSCP) technique. Results: p53 protein expression was detected in all 10 LCH biopsy specimens examined. It was restricted to Langerhans cells (LCH cells), absent from adjacent cells, and localized to the cell nuclei. No mutations of the p53 gene were detected, nor was there abnormal expression of the p53 binding protein, mdm2. Conclusions: p53 is readily detectable in LCH cells but not in normal cells. This is either caused by an unusual mechanism (given the absence of mutations in the p53 gene and of mdm2 expression in LCH cells) or by overexpression or posttranslational changes of normal p53 in response to an as yet unidentified cellular stress, Stabilization and inactivation of p53 could lead to the uncontrolled proliferation of LCH cells, or the abnormality could lead to the induction of programmed cell death. C1 Childrens Natl Med Ctr, Ctr Canc & Transplantat Biol, Washington, DC 20010 USA. NCI, Pediat Branch, NIH, Bethesda, MD 20892 USA. RP Ladisch, S (reprint author), Childrens Natl Med Ctr, Ctr Canc & Transplantat Biol, 111 Michigan Ave NW, Washington, DC 20010 USA. NR 41 TC 36 Z9 38 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 1077-4114 J9 J PEDIAT HEMATOL ONC JI J. Pediatr. Hematol. Oncol. PD JAN-FEB PY 1998 VL 20 IS 1 BP 12 EP 17 DI 10.1097/00043426-199801000-00002 PG 6 WC Oncology; Hematology; Pediatrics SC Oncology; Hematology; Pediatrics GA YX518 UT WOS:000072048600002 PM 9482407 ER PT J AU Yanowitz, TD Yao, AC Werner, JC Pettigrew, KD Oh, W Stonestreet, BS AF Yanowitz, TD Yao, AC Werner, JC Pettigrew, KD Oh, W Stonestreet, BS TI Effects of prophylactic low-dose indomethacin on hemodynamics in very low birth weight infants SO JOURNAL OF PEDIATRICS LA English DT Article ID PATENT DUCTUS-ARTERIOSUS; BLOOD-FLOW VELOCITY; LEFT-VENTRICULAR OUTPUT; PRETERM INFANTS; INTRAVENTRICULAR HEMORRHAGE; PREMATURE-INFANTS; CEREBRAL-ARTERIES; NEWBORN-INFANTS; DOPPLER MEASUREMENTS; PREVENTION AB Indomethacin decreases cerebral and mesenteric blood flow velocities in premature infants with symptomatic patent ductus arteriosus. Low-dose indomethacin is recommended fur the prevention of intraventricular hemorrhage in very low birth weight infants, The hemodynamic effects of prophylactic indomethacin have not been previously examined. We hypothesized that prophylactic indomethacin does not change cerebral and mesenteric blood flow velocities and cardiac Function in very low birth weight infants, Twenty-one infants (775 to 1245 gm, 24 to 31 weeks' gestation) were studied before and after indomethacin (0.1 mg/kg) administration at 6, 30, and 54 hours of life. Mean and end-diastolic cerebral and mesenteric blued flow velocities decreased (ANOVA p < 0.05) after prophylactic indomethacin. The 38% increase in cerebral relative vascular resistance was significantly greater than the 18% increase in mesenteric relative vascular resistance (ANOVA, p < 0.05). In five infants who were fed 1 hour after the third indomethacin dose, the postprandial mesenteric blood flow velocity was significantly greater than the mesenteric blood flow velocity before both indomethacin and feeding (ANOVA, p < 0.05). Cardiac output, stroke volume, fractional shortening, nd blood pressure did not change after prophylactic indomethacin administration. We conclude that prophylactic indomethacin (I) reduces cerebral and mesenteric blood now velocity without affecting cardiac function, (2) increases cerebral more than mesenteric relative vascular resistance, and (3) does not prevent postprandial increases in mesenteric blood flow velocity. We speculate that the increase in cerebral relative vascular resistance is a beneficial effect that contributes to protection against intraventricular hemorrhage. C1 Brown Univ, Women & Infants Hosp, Sch Med, Dept Pediat, Providence, RI 02905 USA. Rhode Isl Hosp, Providence, RI USA. SUNY Hlth Sci Ctr, Dept Pediat, Childrens Med Ctr, Brooklyn, NY USA. NIMH, Div Epidemiol & Serv Res, Bethesda, MD 20892 USA. RP Stonestreet, BS (reprint author), Brown Univ, Women & Infants Hosp, Sch Med, Dept Pediat, 101 Dudley St, Providence, RI 02905 USA. NR 42 TC 29 Z9 30 U1 0 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-3476 EI 1097-6833 J9 J PEDIATR-US JI J. Pediatr. PD JAN PY 1998 VL 132 IS 1 BP 28 EP 34 DI 10.1016/S0022-3476(98)70480-9 PG 7 WC Pediatrics SC Pediatrics GA YV173 UT WOS:000071796700007 PM 9469996 ER PT J AU Taylor, GW Burt, BA Becker, MP Genco, RJ Shlossman, M Knowler, WC Pettitt, DJ AF Taylor, GW Burt, BA Becker, MP Genco, RJ Shlossman, M Knowler, WC Pettitt, DJ TI Non-insulin dependent diabetes mellitus and alveolar bone loss progression over 2 years SO JOURNAL OF PERIODONTOLOGY LA English DT Article DE periodontitis; diabetes mellitus; diabetes mellitus, non-insulin dependent; longitudinal studies; epidemiology; alveolar bone loss ID PERIODONTAL-DISEASE; PIMA-INDIANS; PREVALENCE; MANIFESTATIONS; INDIVIDUALS; ADOLESCENTS; EXPERIENCE; JUVENILE; NIDDM AB THIS STUDY TESTED THE HYPOTHESIS that persons with non-insulin dependent diabetes mellitus (NIDDM) have greater risk of more severe alveolar bone loss progression over a 2-year period than those without NIDDM. Data from the longitudinal study of the oral health of residents of the Gila River Indian Community were analyzed for 362 subjects, aged 15 to 57, 338 of whom had less than 25% radiographic bone loss at baseline, and who did not develop NIDDM nor lose any teeth during the 2-year study period. The other 24 subjects had NIDDM at baseline, but met the other selection criteria. Bone scores (scale 0-4) from panoramic radiographs corresponded to bone loss of 0%, 1%-24%, 25%-49%, 50%-74%, or 75% and greater. Change in bone score category was computed as the change in worst bone score (WBS) reading after 2 years. Age, calculus, NIDDM status, time to follow-up examination, and baseline WBS were explanatory variables in regression models for ordinal categorical response variables. NIDDM was positively associated with the probability of a change in bone score when the covariates were controlled. The cumulative odds ratio for NIDDM at each threshold of the ordered response was 4.23 (95% C.I. = 1.80, 9.92). In addition to being associated with the incidence of alveolar bone loss (as demonstrated in previous studies), these results suggest an NIDDM-associated increased rate of alveolar bone loss progression. C1 Univ Michigan, Sch Dent, Dept Cardiol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA. Univ Michigan, Sch Publ Hlth, Ann Arbor, MI 48109 USA. SUNY Buffalo, Buffalo, NY 14260 USA. NIDDKD, Phoenix, AZ USA. RP Taylor, GW (reprint author), Univ Michigan, Sch Dent, Dept Cardiol Restorat Sci & Endodont, 1011 N Univ, Ann Arbor, MI 48109 USA. FU NIDCR NIH HHS [DE06514, DE07157] NR 44 TC 120 Z9 134 U1 1 U2 7 PU AMER ACAD PERIODONTOLOGY PI CHICAGO PA 737 NORTH MICHIGAN AVENUE, SUITE 800, CHICAGO, IL 60611-2690 USA SN 0022-3492 J9 J PERIODONTOL JI J. Periodont. PD JAN PY 1998 VL 69 IS 1 BP 76 EP 83 PG 8 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA YW039 UT WOS:000071889500012 PM 9527565 ER PT J AU Soldner, A Spahn-Langguth, H Mutschler, E AF Soldner, A Spahn-Langguth, H Mutschler, E TI HPLC assays to simultaneously determine the angiotensin-AT(1) antagonist losartan as well as its main and active metabolite EXP3174 in biological material of humans and rats SO JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS LA English DT Article DE losartan; EXP 3174; nonpeptide Angiotensin-AT(1) antagonists; reversed-phase high-performance liquid chromatography; solid-phase extraction; plasma; urine; blood; bile; tissues; rats ID II RECEPTOR ANTAGONISTS; DUP-753; AGENT AB Novel rapid and sensitive HPLC assays were developed to simultaneously determine losartan and its main active metabolite EXP 3174 in biological material of humans and rats following solid-phase or liquid-liquid extraction. The analytes were separated on a 3 mu m particle-sized ULTREMEX(TM) CN column, which was preceeded by a 5 mu m particle-sized guard column, using UV-detection at 245 nm. The assays provided high sensitivity with limits of quantification (LoQ) of 5 ng ml(-1) for both compounds in human and rat plasma and 10 ng ml(-1) in human and rat urine, respectively. In rat blood, bile and various tissues, limits of quantifications were achieved that ranged 10-15 ng per mi and per 100 mg tissue, respectively, for both analytes. (C) 1998 Elsevier Science B.V. C1 Univ Frankfurt, Bioctr Niederursel, Dept Pharmacol, D-60439 Frankfurt, Germany. Univ Halle Wittenberg, Dept Pharmaceut Chem, D-06120 Halle, Germany. RP Soldner, A (reprint author), NIDDKD, Bioorgan Chem Lab, NIH, 8A-BIA-09, Bethesda, MD 20892 USA. NR 8 TC 57 Z9 60 U1 0 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0731-7085 J9 J PHARMACEUT BIOMED JI J. Pharm. Biomed. Anal. PD JAN PY 1998 VL 16 IS 5 BP 863 EP 873 DI 10.1016/S0731-7085(97)00128-3 PG 11 WC Chemistry, Analytical; Pharmacology & Pharmacy SC Chemistry; Pharmacology & Pharmacy GA YZ902 UT WOS:000072305900018 PM 9535198 ER PT J AU Tirelli, E Geter-Douglass, B Witkin, JM AF Tirelli, E Geter-Douglass, B Witkin, JM TI gamma-aminobutyric acid(A) agonists differentially augment gnawing induced by indirect-acting dopamine agonists in C57BL/6J mice SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID BIOGENIC-AMINE TRANSPORTERS; LIGAND-BINDING DOMAINS; H-3 MAZINDOL BINDING; DISCRIMINATIVE-STIMULUS; UPTAKE INHIBITORS; REUPTAKE INHIBITORS; COCAINE ANTAGONISTS; NONHUMAN-PRIMATES; RECEPTOR AGONISTS; PREFRONTAL CORTEX AB Evidence from structure-activity, molecular biology, ligand binding and behavioral studies has suggested potential differences in the pharmacological effects of indirect dopamine agonists. Striatal dopaminergic neurotransmission is under the regulatory control of GABAergic inputs. The ability of agonists of gamma-aminobutyric acid(A) (GABA(A)) receptors to enhance stereotyped gnawing was used as a method for dissociating the pharmacological effects of indirect-acting dopamine agonists. Gnawing on corrugated cardboard was studied in C57BL/6J mice. The GABA(A) agonists, gaboxadol HCl (THIP) and muscimol, were not effective in augmenting gnawing in the presence of the direct-acting dopamine agonists, apomorphine, pergolide, RU 24213 or SKF 38393. In addition, THIP did not enhance the gnawing produced by cocaine, bupropion, GBR 12909 or WIN 35428. In contrast, THIP produced marked augmentation of the gnawing induced by methylphenidate, (+)-amphetamine, methamphetamine, amfonelic acid, indatraline, nomifensine, diclofensine, mazindol and GBR 12935. The qualitative differences in potentiation were not caused by differences in the maximal effect of the drugs alone, inadequate dose or routes of administration, or by differences in duration of action. Neither can the absence of potentiation be accounted for by unique effects of THIP; muscimol was only marginally effective in potentiating the effects of WIN 35428 and bupropion but completely inactive in augmenting the effects of cocaine and GBR 12909. Muscimol was efficacious in augmenting the effects of the drugs for which THIP was active. These results add to a small but growing literature that demonstrates differences in the in vitro and in vivo pharmacological effects of indirect dopamine agonists. The methods used here may help in defining the molecular and neural substrates of these differential effects. C1 NIDA, Drug Dev Grp, Preclin Pharmacol Lab, Addict Res Ctr,NIH, Baltimore, MD 21224 USA. RP Witkin, JM (reprint author), NIDA, Drug Dev Grp, Preclin Pharmacol Lab, Addict Res Ctr,NIH, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. NR 73 TC 6 Z9 6 U1 1 U2 1 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD JAN PY 1998 VL 284 IS 1 BP 116 EP 124 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA YQ672 UT WOS:000071411700017 PM 9435169 ER PT J AU Felder, CC Joyce, KE Briley, EM Glass, M Mackie, KP Fahey, KJ Cullinan, GJ Hunden, DC Johnson, DW Chaney, MO Koppel, GA Brownstein, M AF Felder, CC Joyce, KE Briley, EM Glass, M Mackie, KP Fahey, KJ Cullinan, GJ Hunden, DC Johnson, DW Chaney, MO Koppel, GA Brownstein, M TI LY320135, a novel cannabinoid CB1 receptor antagonist, unmasks coupling of the CB1 receptor to stimulation of cAMP accumulation SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID MEDIATED SIGNAL-TRANSDUCTION; BETA-GAMMA-SUBUNITS; ADENYLATE-CYCLASE; RAT-BRAIN; CALCIUM CURRENTS; CELLS; ANANDAMIDE; DELTA-9-TETRAHYDROCANNABINOL; LOCALIZATION; MEMBRANES AB LY320135 is a selective antagonist for the brain CB1 receptor, having greater than 70-fold higher affinity for the CB1 than the peripheral CB2 receptor. The K-i values for LY320135 at the CB1 and CB2 receptors, transfected and stably expressed in cell lines, were 224 nM and >10 mu M, respectively. Similar K-i values were measured in binding studies performed on cerebellum and spleen membrane preparations endogenously expressing the CB1 (203 nM) and CB2 (>10 mu M) receptors, respectively. LY320135 functionally reversed anandamide-mediated adenylate cyclase inhibition in Chinese hamster ovary (CHO) cells stably expressing the CB1 receptor. Pertussis toxin treatment of CHO cells expressing the CB1 receptor attenuated the anandamide-mediated inhibition of adenylate cyclase and unmasked a stimulatory effect of anandamide on adenylate cyclase. The stimulatory component was blocked with LY320135. This compound also blocked WIN 55212-2-mediated inhibition of N-type calcium channels and activation of inwardly rectifying potassium channels in N18 and AtT-20-CB2 cells, respectively. LY320135 is a promising lead compound for the further development of novel, potent and selective cannabinoid antagonists of novel structure. C1 Eli Lilly & Co, Lilly Corp Ctr, Lilly Res Labs, Div Neurosci, Indianapolis, IN 46285 USA. Eli Lilly & Co, Lilly Corp Ctr, Lilly Res Labs, Div Endocrine, Indianapolis, IN 46285 USA. NIMH, Lab Cellular & Mol Regulat, Bethesda, MD 20892 USA. NIMH, Cell Biol Lab, Bethesda, MD 20892 USA. Univ Washington, Dept Anesthesiol, Seattle, WA 98195 USA. Univ Washington, Dept Physiol, Seattle, WA 98195 USA. RP Felder, CC (reprint author), Eli Lilly & Co, Lilly Corp Ctr, Lilly Res Labs, Div Neurosci, Drop 0510, Indianapolis, IN 46285 USA. RI Brownstein, Michael/B-8609-2009; Mackie, Kenneth/B-7358-2011; Mackie, Ken/E-3715-2013; OI Mackie, Ken/0000-0001-8501-6199; Glass, Michelle/0000-0002-5997-6898 FU NIDA NIH HHS [DA09203]; NINDS NIH HHS [NS01588, NS08174] NR 36 TC 177 Z9 188 U1 0 U2 0 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD JAN PY 1998 VL 284 IS 1 BP 291 EP 297 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA YQ672 UT WOS:000071411700038 PM 9435190 ER PT J AU Ferguson, RJ De Morais, SMF Benhamou, S Bouchardy, C Blaisdell, J Ibeanu, G Wilkinson, GR Sarich, TC Wright, JM Dayer, P Goldstein, JA AF Ferguson, RJ De Morais, SMF Benhamou, S Bouchardy, C Blaisdell, J Ibeanu, G Wilkinson, GR Sarich, TC Wright, JM Dayer, P Goldstein, JA TI A new genetic defect in human CYP2C19: Mutation of the initiation codon is responsible for poor metabolism of S-mephenytoin SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID HYDROXYLATION POLYMORPHISM; OXIDATION POLYMORPHISM; CHINESE SUBJECTS; EXPRESSION; PROTEIN; DEBRISOQUIN; DEFICIENCY; PHENOTYPE; SUBFAMILY; JAPANESE AB The 4'-hydroxylation of the S-enantiomer of the anticonvulsant drug mephenytoin exhibits a genetic polymorphism in humans. This polymorphism shows marked interracial heterogeneity, with the poor metabolizer (PM) phenotype representing 2 to 5% of Caucasian and 13 to 23% of Asian populations. Two defective CYP2C19 alleles, CYP2C19*2 and CYP2C19*3, have been described which account for similar to 87% of Caucasian and >99% of Oriental PM alleles. The present study identifies a new allele (CYP2C19*4) in Caucasian PMs which contains an A --> G mutation in the initiation codon. A new polymerase chain reaction-restriction fragment length polymorphism genotyping test was developed, and the incidence of this allele was examined in a European Caucasian population which had been phenotyped for mephenytoin metabolism. One of nine putative PMs was heterozygous for CYP2C19*2/CYP2C19*4, which suggests that CYP2C19*4 represents a defective allele. Six of the seven remaining putative PMs available for genotyping were explained by CYP2C19*2. The frequency of the CYP2C19*4 allele in Caucasians was 0.6%. An additional Caucasian PM from a separate study was also heterozygous for CYP2C19*2 and CYP2C19*4. To verify that CYP2C19*4 represented a defective CYP2C19 allele, the initiation codon of the normal CYP2C19*1 cDNA was mutated to a GTG, and both cDNAs were expressed in yeast. Recombinant CYP2C19 protein was detected by Western blot analysis of colonies transformed with CYP2C19*1 cDNA, but not in those transformed with CYP2C19*4 cDNA. The two cDNAs were also used in an in vitro coupled transcription/translation assay. CYP2C19 protein was translated only from the CYP2C19*1 allele. These data indicate that CYP2C19*4 represents a new PM allele. C1 NIEHS, Res Triangle Pk, NC 27709 USA. INSERM, U351, Villejuif, France. Geneva Canc Registry, Geneva, Switzerland. Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN 37212 USA. Univ British Columbia, Dept Pharmacol & Therapeut, Vancouver, BC V6T 1W5, Canada. Univ Hosp Geneva, Geneva, Switzerland. RP Goldstein, JA (reprint author), NIEHS, POB 12233, Res Triangle Pk, NC 27709 USA. RI Benhamou, Simone/K-6554-2015; OI Sarich, Troy/0000-0003-0909-9839 FU NIGMS NIH HHS [GM31304]; PHS HHS [32165] NR 40 TC 152 Z9 160 U1 0 U2 6 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD JAN PY 1998 VL 284 IS 1 BP 356 EP 361 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA YQ672 UT WOS:000071411700046 PM 9435198 ER PT J AU Yang, YW Teng, CC AF Yang, YW Teng, CC TI Circular dichroism and fluorescence studies of polyomavirus major capsid protein VP1 SO JOURNAL OF PROTEIN CHEMISTRY LA English DT Article DE polyomavirus; VP1; circular dichroism; fluorescence ID CONFORMATION; PREDICTION; RESOLUTION; PEPTIDE; PH AB The conformational changes of polymavirus (Py) major capsid protein VP1 in solution by the solution pH, addition of calcium, and ionic strength were examined by circular dichroism (CD) and fluorescence spectroscopy. Comparison of the predicted secondary structures of PyVP1 and simian virus (SV) 40 by the methods of Chou-Fasman, Garnier et al., and Yang method are presented, Hydropathicity, surface probability, and chain flexibility of PyVP1 were computer-analyzed by the methods of Kyte and Doolittle, Emini et al., and Karplus and Schulz, respectively, The CD measurements indicate that the secondary structure of PyVP1 is little dependent on its concentration, Ca2+ concentration, and ionic strength, but is strongly pH dependent, Fluorescence studies showed that emission spectra of PyVP1 are also pH-dependent. At extreme acidic and alkaline pH, the fluorescence intensity of PyVP1 is decreased and the emission maximum is red-shifted, The fluorescence of PyVP1 is quenched by the presence of CsCl, KI, and acrylamide. The analyses of the modified Stern-Volmer plots indicate that five of seven tryptophan residues in PyVP1 are located on the surface of the protein, among which two are accessible to Cs+ and the other three are accessible to I-=. The two others are buried more deeply in the interior of the protein molecule. C1 Natl Taiwan Univ, Coll Med, Sch Pharm, Taipei 100, Taiwan. RP Yang, YW (reprint author), NHLBI, Mol Hematol Branch, NIH, Bldg 10-7D05,10 Ctr Dr, Bethesda, MD 20892 USA. NR 21 TC 5 Z9 5 U1 1 U2 3 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0277-8033 J9 J PROTEIN CHEM JI J. Protein Chem. PD JAN PY 1998 VL 17 IS 1 BP 61 EP 71 DI 10.1023/A:1022542631609 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA YV988 UT WOS:000071884500008 PM 9491929 ER PT J AU Gurguis, GNM Uhde, TW AF Gurguis, GNM Uhde, TW TI The relationship between plasma MHPG and NE: employing regression models in estimating centrally derived MHPG and peripheral NE turnover rate in panic disorder SO JOURNAL OF PSYCHIATRIC RESEARCH LA English DT Article ID 3-METHOXY-4-HYDROXYPHENYLETHYLENE GLYCOL MHPG; ALPHA-2-ADRENERGIC RECEPTOR-BINDING; GENERALIZED ANXIETY DISORDER; NORADRENERGIC FUNCTION; CEREBROSPINAL-FLUID; HEALTHY-SUBJECTS; HEART-RATE; NOREPINEPHRINE METABOLISM; ELECTROCHEMICAL DETECTION; ANTIANXIETY TREATMENT AB Studies investigating the role of the noradrenergic system in the pathophysiology of anxiety have focused on measuring plasma 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) levels. Fewer studies have examined norepinephrine levels. Basal plasma norepinephrine and free MHPG levels were simultaneously measured in 33 normal controls and 20 panic disorder (PD) patients. Norepinephrine levels were similar in patients and controls, but MHPG levels were significantly lower in patients (13.34 +/- 3.22 vs 18.37 +/- 4.49 pmol ml(-1), p < 0.0001). Norepinephrine correlated significantly with plasma MHPG levels in controls (r=0.538, p<0.0001) and patients (r=0.645, p<0.002). Patients had a trend toward a lower y-intercept than controls, suggesting a lower contribution by the CNS to MHPG pool plasma levels (9.18 vs 12.51,p<0.08). Norepinephrine turnover rate was similar in patients and controls. We propose that the dysregulation in the noradrenergic system in PD may be akin to animal studies of acute-on-top-of-chronic stress paradigms, whereby chronic stress results in normal or decreased basal NE turnover and sensitized responses to recurrent stresses. (C) 1998 Elsevier Science Ltd. All rights reserved. C1 NIMH, Biol Psychiat Branch, Anxiety & Affect Disorders Sect, Bethesda, MD 20892 USA. RP Gurguis, GNM (reprint author), NIMH, Biol Psychiat Branch, Anxiety & Affect Disorders Sect, Bethesda, MD 20892 USA. NR 72 TC 6 Z9 6 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-3956 J9 J PSYCHIAT RES JI J. Psychiatr. Res. PD JAN-FEB PY 1998 VL 32 IS 1 BP 11 EP 17 DI 10.1016/S0022-3956(97)00036-8 PG 7 WC Psychiatry SC Psychiatry GA 100TH UT WOS:000074831100002 PM 9693996 ER PT J AU Kanik, KS Hagiwara, E Yarboro, CH Schumacher, HR Wilder, RL Klinman, DM AF Kanik, KS Hagiwara, E Yarboro, CH Schumacher, HR Wilder, RL Klinman, DM TI Distinct patterns of cytokine secretion characterize new onset synovitis versus chronic rheumatoid arthritis SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE rheumatoid arthritis; cytokines; immunology; interferon-gamma; interleukin 2; tumor necrosis factor-alpha ID SYSTEMIC LUPUS-ERYTHEMATOSUS; T-CELL CLONES; LYMPHOKINE ACTIVITIES; TH1; AUTOIMMUNITY; PROFILES; DISEASES; ALPHA; BLOOD AB Objective. To compare patterns of cytokine secretion in patients with new onset synovitis (< 1 yr duration, n = 14), chronic rheumatoid arthritis (RA) (n = 16), and healthy controls (n = 17). Methods. ELIspot assays were used to detect mononuclear cells in the peripheral blood (PMBC) and synovial fluid (SFMC) secretion the type 1 cytokines interleukin 2 (IL-2) and interferon-gamma (IFN-gamma), the type 2 cytokines IL-4, IL-6 and IL-10, and the inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). Results were correlated with measures of disease activity. Results. Patients with new onset synovitis had increased (p < 0.05) numbers of PBMC secreting IL-2. The number of PBMC secreting IFN-gamma correlated with the joint score in the new onset synovitis population (p = 0.006). By comparison, patients with chronic RA had significantly increased numbers of PBMC secreting IL-6, IL-10, and TNF-alpha (p < 0.05). The production of these cytokines correlated with joint score in chronic RA (p = 0.008, 0.06, 0.001, respectively). Conclusion. Patients with new onset synovitis have increased numbers of PBMC secreting IL-2 and IFN-gamma, while patients with chronic RA have increased numbers of PBMC secreting IL-6, IL-10, and TNF-alpha. Correlations between joint score and number of PBMC secreting cytokines suggest the number of PBMC secreting IFN-gamma is most relevant in new onset synovitis, while the number of PBMC secreting IL-6, IL-10, and TNF-alpha is of greater relevance in chronic RA. C1 NIAMS, Inflammatory Joint Dis Sect, Arthrit & Rheumatism Branch, NIH, Bethesda, MD USA. US FDA, Sect Retroviral Res, Div Viral Prod, Ctr Biol Evaluat & Res, Bethesda, MD 20014 USA. RP Kanik, KS (reprint author), Univ S Florida, Coll Med, Div Rheumatol, 12901 Bruce B Downs Blvd, Tampa, FL 33612 USA. NR 28 TC 43 Z9 53 U1 1 U2 1 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO, ONTARIO M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD JAN PY 1998 VL 25 IS 1 BP 16 EP 22 PG 7 WC Rheumatology SC Rheumatology GA YQ723 UT WOS:000071416800005 PM 9458197 ER PT J AU Hagiwara, E Pando, J Ishigatsubo, Y Klinman, DM AF Hagiwara, E Pando, J Ishigatsubo, Y Klinman, DM TI Altered frequency of type 1 cytokine secreting cells in the peripheral blood of patients with primary Sjogren's syndrome SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE Sjogren's syndrome; type 1 cytokine; peripheral blood mononuclear cells; ELISPOT assay ID SYSTEMIC LUPUS-ERYTHEMATOSUS; MRL/LPR MICE; IMMUNE REGULATION; SALIVARY-GLANDS; FLOW-CYTOMETRY; HIV-INFECTION; T-CELLS; EXPRESSION; IL-10; AUTOANTIBODIES AB Objective. An imbalance in immunoregulatory cytokines may contribute to the etiopathogenesis of Sjogren's syndrome (SS). We investigated systemic abnormalities in cytokine production in the peripheral blood in patients with SS. Methods. ELISPOT assays were used to detect and enumerate cells spontaneously secreting interleukin 2 (IL-2), IL-6, IL-10, and interferon-gamma (IFN-gamma) in freshly isolated peripheral blood mononuclear cells from 20 patients with SS and 20 healthy controls. Results. The number of cells spontaneously secreting type 1 cytokines IL-2 and IFN-gamma was decreased in the peripheral blood of patients with SS compared to controls. There was no change observed in the number of cells spontaneously secreting IL-6 and IL-10. Cells spontaneously secreting IL-4 were too rare in peripheral blood to evaluate, although cells capable of secreting IL-4 in response to phytohemagglutinin did not differ from controls. Patients with severe extraglandular symptoms (such as vasculitis) had a significantly lower frequency of IFN-gamma secreting cells in their peripheral blood than those without extraglandular involvement. Conclusion. These results suggest that decreased type 1 cytokine production may contribute to or reflect the pathogenesis of SS. C1 Yokohama City Univ, Sch Med, Dept Internal Med 1, Kanazawa Ku, Yokohama, Kanagawa 236, Japan. US FDA, Sect Retroviral Immunol, Ctr Biol Evaluat & Res, Bethesda, MD 20014 USA. NIAMSD, NIH, Bethesda, MD 20892 USA. RP Hagiwara, E (reprint author), Yokohama City Univ, Sch Med, Dept Internal Med 1, Kanazawa Ku, 3-9 Fukuura, Yokohama, Kanagawa 236, Japan. NR 41 TC 33 Z9 34 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO, ONTARIO M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD JAN PY 1998 VL 25 IS 1 BP 89 EP 93 PG 5 WC Rheumatology SC Rheumatology GA YQ723 UT WOS:000071416800017 PM 9458209 ER PT J AU Steinert, PM Candi, E Kartasova, T Marekov, L AF Steinert, PM Candi, E Kartasova, T Marekov, L TI Small proline-rich proteins are cross-bridging proteins in the cornified cell envelopes of stratified squamous epithelia SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article DE barrier function; cornified cell envelope; epidermis; involucrin; loricrin; transglutaminases ID HUMAN EPIDERMAL-KERATINOCYTES; HUMAN LORICRIN; DIFFERENTIAL EXPRESSION; LAMELLAR ICHTHYOSIS; INVOLUCRIN; TISSUES; FAMILY; TRANSGLUTAMINASES; COMPONENTS; RETINOIDS AB The cornified cell envelope (CE) is a specialized structure which contributes barrier function to stratified squamous epithelial cells. It is composed of an amalgam of several structural proteins that are rendered insoluble by isopeptide bond crosslinking by transglutaminases, One set of the structural proteins present in CEs of most such epithelia are the small proline rich (SPR) proteins, which are a family of about 12 related structural proteins. We have recovered a large number of peptides containing isopeptide crosslinks, including 236 involving SPR proteins, following proteolysis of CEs isolated from foreskin epidermal tissue and cultured epidermal keratinocytes. Analysis of this database has provided novel information on their function. First, we found that SPRs became crosslinked to many other structural proteins within the CE. Second, multiple glutamine and lysine residues located only on the amino- and carboxy-termini of the SPR proteins were involved in crosslinking, so that the two ends are functionally equivalent. Third, the SPRs functioned as cross-bridging proteins, by directly adjoining other CE structural proteins. In the specialized case of the epidermal CE, the SPRs cross-bridged between loricrin. In cultured keratinocytes which make little loricrin and serve as a model for internal stratified squamous epithelia, the SPRs formed extensive cross-bridges among themselves. Thus SPRs are ubiquitous cross-bridging proteins whose differential expression patterns apparently reflect specific barrier requirements of different epithelia. C1 NIAMSD, Skin Biol Lab, NIH, Bethesda, MD 20892 USA. RP Steinert, PM (reprint author), NIAMSD, Skin Biol Lab, NIH, Bethesda, MD 20892 USA. NR 36 TC 60 Z9 61 U1 1 U2 2 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PY 1998 VL 122 IS 1-2 BP 76 EP 85 DI 10.1006/jsbi.1998.3957 PG 10 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 116VZ UT WOS:000075746900007 PM 9724607 ER PT J AU Levine, RJC Yang, ZH Epstein, ND Fananapazir, L Stull, JT Sweeney, HL AF Levine, RJC Yang, ZH Epstein, ND Fananapazir, L Stull, JT Sweeney, HL TI Structural and functional responses of mammalian thick filaments to alterations in myosin regulatory light chains SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article DE thick filaments; exchanged; expressed; wild-type; mutant myosin regulatory light chains; familial hypertrophic cardiomyopathy ID SKELETAL-MUSCLE MYOSIN; HYPERTROPHIC CARDIOMYOPATHY; GLYCOGEN-PHOSPHORYLASE; STRIATED-MUSCLE; BETA-MYOSIN; EXPRESSION AB The ordered array of myosin heads, characteristic of relaxed striated muscle thick filaments, is reversibly disordered by phosphorylating myosin regulatory light chains, decreasing temperature and/or ionic strength, increasing pH, and depleting nucleotide, In the case of light chain phosphorylation, disorder, most likely due to a change in charge affecting the light chain amino-terminus, reflects increased myosin head mobility, thus increased accessibility to actin, and results in increased calcium sensitivity of tension development. Thus, interactions between the unphosphorylated regulatory light chain and the filament backbone may help maintain the overall order of the relaxed filament, To define this relationship, we have examined the structural and functional effects of such manipulations as exchanging wild-type smooth and skeletal myosin light chains into permeabilized rabbit psoas fibers and removing regulatory light chains (without exchange) from such fibers, We have also compared the structural and functional parameters of biopsied fibers from patients with severe familial hypertrophic cardiomyopathy due to a single amino acid substitution in the regulatory light chains to those exhibited by fibers from normal relatives. Our results support a role for regulatory light chains in reversible ordering of myosin heads and suggest that economy of energy utilization may provide for evolutionary preservation of this function in vertebrate striated muscle. (C) 1998 Academic Press. C1 Allegheny Univ Hlth Sci, Hahnemann Sch Med, MCP, Dept Neurobiol & Anat, Philadelphia, PA 19129 USA. Univ Penn, Sch Med, Dept Physiol, Philadelphia, PA 19104 USA. NHLBI, NIH, Bethesda, MD 20892 USA. Univ Texas, SW Med Sch, Dept Physiol, Dallas, TX 75235 USA. RP Levine, RJC (reprint author), Allegheny Univ Hlth Sci, Hahnemann Sch Med, MCP, Dept Neurobiol & Anat, 3200 Henry Ave, Philadelphia, PA 19129 USA. RI Sweeney, H Lee/F-1862-2010 FU NHLBI NIH HHS [HL06296]; NIAMS NIH HHS [AR35661] NR 27 TC 53 Z9 53 U1 0 U2 2 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PY 1998 VL 122 IS 1-2 BP 149 EP 161 DI 10.1006/jsbi.1998.3980 PG 13 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 116VZ UT WOS:000075746900016 PM 9724616 ER PT J AU Khan, S Zhao, RB Reese, TS AF Khan, S Zhao, RB Reese, TS TI Architectural features of the Salmonella typhimurium flagellar motor switch revealed by disrupted C-rings SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article ID BASAL BODY; ESCHERICHIA-COLI; ELECTRON-MICROSCOPY; PROTONMOTIVE FORCE; QUICK-FREEZE; MOTILITY; PROTEIN; CHEMOTAXIS; COMPLEX; FLIG AB The three-dimensional surface topology of rapid-frozen Salmonella typhimurium flagellar hook, basal body complexes was studied by stereo-examination of thin-film metal replicas. The complexes contained the extended cytoplasmic structure, composed of the switch complex proteins; FliG, FliM, and FliN. Distinct nanometer-scale element arrays, separated by grooves, defined the outer surface of the cytoplasmic (C-) ring. The number of array elements was comparable to previously determined FliG and FliM copy numbers in the basal body. In addition to basal body complexes lacking C-rings, complexes containing incomplete C-rings were identified. The incomplete C-rings had lost segments of the proximal array. Basal bodies with the distal C-ring array alone were not found. These findings are compatible with the spatial organization of the flagellar switch suggested by previous biochemical data. (C) 1998 Academic Press. C1 Albert Einstein Coll Med, Dept Physiol & Biophys, Bronx, NY 10461 USA. NINDS, Neurobiol Lab, NIH, Bethesda, MD 20892 USA. RP Khan, S (reprint author), Albert Einstein Coll Med, Dept Physiol & Biophys, 1300 Morris Pk Ave, Bronx, NY 10461 USA. FU NIGMS NIH HHS [GM36936] NR 33 TC 11 Z9 11 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PY 1998 VL 122 IS 3 BP 311 EP 319 DI 10.1006/jsbi.1998.3999 PG 9 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 123WJ UT WOS:000076148300006 PM 9774535 ER PT J AU Watts, NR Misra, M Wingfield, PT Stahl, SJ Cheng, NQ Trus, BL Steven, AC Williams, RW AF Watts, NR Misra, M Wingfield, PT Stahl, SJ Cheng, NQ Trus, BL Steven, AC Williams, RW TI Three-dimensional structure of HIV-1 Rev protein filaments SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article DE AIDS; cryo-electron microscopy; helical filaments; HIV-1; image reconstruction; Rev ID IMMUNODEFICIENCY-VIRUS TYPE-1; SECONDARY STRUCTURE-ANALYSIS; RAY FIBER DIFFRACTION; NUCLEOCYTOPLASMIC TRANSPORT; REGULATORY PROTEINS; RESPONSE ELEMENT; GENE-EXPRESSION; MOSAIC-VIRUS; BINDING; RNA AB The HIV-1 Rev protein facilitates the export of incompletely spliced and unspliced viral mRNAs from the nucleus. Rev polymerizes into two types of filaments in vitro. In the presence of RNA, Rev forms poorly ordered structures, while in the absence of RNA it polymerizes into regular hollow filaments. We have determined the helical structure of the latter filaments by analysis of cryo-electron micrographs, taking into account STEM measurements of mass-per-unit-length. They are made up of Rev dimers, arranged in a six-start helix, with 31 dimers in 2 turns, a pitch angle of 45 degrees, and an interstrand spacing of 3.8 nm. Three-dimensional reconstruction at 2.1 nm resolution reveals a smooth outer surface and a featured inner surface, with outer and inner diameters of similar to 14.8 and similar to 10.4 nm, respectively. The Rev dimer has a "top-hat" shape with a cylinder similar to 3.2 nm in diameter and similar to 2.2 nm high, pointing inward: the thinner rim areas pack together to form the filament wall. Raman spectroscopy shows polymerized Rev to have similar to 54% alpha-helix and 20-24% beta-sheet content, Electron microdiffraction of aligned filaments reveals a broad meridional reflection at similar to (0.51 nm)(-1), suggesting approximate alignment of the alpha-helices with the filament axis. Based on these data, a molecular model for the Rev filament is proposed. C1 NIH, Bethesda, MD 20892 USA. NIAMSD, Prot Express Lab, Bethesda, MD 20892 USA. NIAMSD, Struct Biol Res Lab, Bethesda, MD 20892 USA. Div Comp Res & Technol, Computat Biosci & Engn Lab, Bethesda, MD 20892 USA. Uniformed Serv Univ Hlth Sci, Dept Biochem, Bethesda, MD 20814 USA. RP Steven, AC (reprint author), NIH, Bldg 6,Room B2-34,6 Ctr Dr MSC 2717, Bethesda, MD 20892 USA. EM steven@calvin.niams.nih.gov NR 54 TC 35 Z9 35 U1 1 U2 2 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PD JAN PY 1998 VL 121 IS 1 BP 41 EP 52 DI 10.1006/jsbi.1998.3964 PG 12 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA ZJ238 UT WOS:000073193800005 PM 9573619 ER PT J AU Durell, SR Hao, YL Guy, HR AF Durell, SR Hao, YL Guy, HR TI Structural models of the transmembrane region of voltage-gated and other K(+) channels in open, closed, and inactivated conformations SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article DE potassium channels; ion pores; molecular models; protein structure ID SHAKER POTASSIUM CHANNELS; ACETYLCHOLINE-RECEPTOR; SELECTIVITY FILTER; ION CONDUCTION; PORE STRUCTURE; GATING CHARGE; BINDING-SITES; TEA BLOCKADE; PROTEINS; RESIDUES AB A large collaborative, multidisciplinary effort involving many research laboratories continues which uses indirect methods of molecular biology and membrane biophysics to analyze the three-dimensional structures and functional mechanisms of K(+) channels. This work also extends to the distant relatives of these channels, including the voltage-gated Na(+) and Ca(2+) channels. The role that our group plays in this process is to combine the information gained from experimental studies with molecular modeling techniques to generate atomic-scale structural models of these proteins. The modeling process involves three stages which are summarized as: (I) prediction of the channel sequence transmembrane topology, including the functionality and secondary structure of the segments; (II) prediction of the relative positions of the transmembrane segments, and (III) filling in all atoms of the amino acid residues, with conformations for energetically stabilized interactions. Both physiochemical and evolutionary principles (including sequence homology analysis) are used to guide the development. In addition to testing the steric and energetic feasibilities of different structural hypotheses, the models provide guidance for the design of new experiments. Structural modeling also serves to "fill in the gaps" of experimental data, such as predicting additional residue interactions and conformational changes responsible for functional processes. The modeling process is currently at the stage that experimental studies have definitely confirmed most of our earlier predictions about the transmembrane topology and functionality of different segments. Additionally, this report describes the detailed, three-dimensional models we have developed for the entire transmembrane region and important functional sites of the voltage-gated Shaker K(+) channel in the open, closed, and inactivated conformations (including the ion-selective pore and voltage-sensor regions). As part of this effort, we also describe how our development of structural models for many of the other major K(+) channel families aids in determining common structural motifs. As an example, we also present a detailed model of the smaller, bacterial K(+) channel from Streptomyces lividans. Finally, we discuss strategies for using newly developed experimental methods for determining the structures and analyzing the functions of these channel proteins. (C) 1998 Academic Press. C1 NCI, Lab Expt & Computat Biol, Div Basic Sci, NIH, Bethesda, MD 20892 USA. RP Durell, SR (reprint author), NCI, Lab Expt & Computat Biol, Div Basic Sci, NIH, Bldg 12B,Room B116, Bethesda, MD 20892 USA. NR 90 TC 86 Z9 88 U1 1 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PY 1998 VL 121 IS 2 BP 263 EP 284 DI 10.1006/jsbi.1998.3962 PG 22 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA ZP657 UT WOS:000073775400016 PM 9615442 ER PT J AU Dawson, DA Grant, BF AF Dawson, DA Grant, BF TI Family history of alcoholism and gender: Their combined effects on DSM-IV alcohol dependence and major depression SO JOURNAL OF STUDIES ON ALCOHOL LA English DT Article ID PSYCHIATRIC-DISORDERS; GENERAL-POPULATION; CO-MORBIDITY; COMORBIDITY; ABUSE; WOMEN AB Objective: Data from a representative sample of U.S. adults were used to assess the extent of familiar alcoholism to examine its association with the odds of DSM-IV lifetime alcohol dependence, major depression, and their comorbid occurrence, and to determine whether the magnitude of this association was different for men and women. Method: Self-report data from a sample of 42,862 U.S. adults (25,043 women) 18 years of age and over were analyzed by means of multiple logistic regression models that predicted the odds of various combinations of DSM-IV alcohol dependence and major depression. Results: After adjusting for potential confounders through multiple logistic regression, family history saturation was associated with increased odds of dependence only, depression only, and all primary-secondary concurrent combinations of these two disorders. The estimated effects were greatest for comorbid dependence and depression, next highest for dependence only and lowest for depression only. Differences in odds ratios among these groups increased with degree of family history saturation but were statistically significant at all levels of saturation. The effects of family history were greater for men than women for the outcome of primary depression followed by secondary dependence, but only at the higher levels of saturation. Among persons with lifetime major depression, family history of alcoholism had a positive independent association with the conditional odds of having experienced comorbid alcohol dependence. It had a weaker but still significant association with the odds of comorbid depression, conditional upon having experienced dependence, and this association was stronger among men than among women. For most outcomes, family history effects were stronger for paternal male and maternal female relatives than for paternal female and maternal male relatives. Conclusions: These findings supported prior research showing more familial alcoholism among persons with comorbid dependence and depression than among those with dependence alone. Gender differences were supportive of the proposed distinction between pure depression and depressive-spectrum disease. RP Dawson, DA (reprint author), NIAAA,BIOMETRY BRANCH,DIV BIOMETRY & EPIDEMIOL,NIH,WILLCO BLDG,SUITE 514,BETHESDA,MD 20892, USA. NR 32 TC 61 Z9 61 U1 3 U2 7 PU ALCOHOL RES DOCUMENTATION INC CENT ALCOHOL STUD RUTGERS UNIV PI PISCATAWAY PA PO BOX 969, PISCATAWAY, NJ 08855-0969 SN 0096-882X J9 J STUD ALCOHOL JI J. Stud. Alcohol PD JAN PY 1998 VL 59 IS 1 BP 97 EP 106 PG 10 WC Substance Abuse; Psychology SC Substance Abuse; Psychology GA YL441 UT WOS:A1998YL44100012 PM 9498321 ER PT J AU Grant, BF AF Grant, BF TI Age at smoking onset and its association with alcohol consumption and DSM-IV alcohol abuse and dependence: Results from the national longitudinal alcohol epidemiologic survey SO JOURNAL OF SUBSTANCE ABUSE LA English DT Article ID DRINKING AB The major purpose of this study was to examine the relationship of early onset smoking with lifetime drinking and the subsequent development of DSM-IV alcohol abuse and dependence using a large representative sample of the U.S. general population. Prevalences of lifetime drinking, alcohol abuse and dependence, and their associated severity were compared among smoking groups defined by age at onset of smoking and among nonsmokers. Linear logistic regression analyses were conducted to. assess the relationship between age at smoking onset and lifetime drinking, alcohol abuse and dependence, controlling for important covariates. Early onset smoking was a significant predictor of lifetime drinking and the subsequent development of lifetime alcohol abuse and dependence, a relationship that generally remained consistent for males, females, whites and blacks. Early onset smoking was significantly associated with more excessive alcohol consumption and more severe alcohol use disorders relative to late onset smokers and nonsmokers. Early onset smoking was also significantly associated with heavier and longer smoking careers compared to late onset smokers. Implications of these findings are discussed in terms of prevention of adolescent smoking and the need for further research on understanding the mechanisms underlying the associations between early onset smoking and lifetime drinking, alcohol abuse and dependence. C1 NIAAA, Div Biometry & Epidemiol, Rockville, MD 20852 USA. RP Grant, BF (reprint author), NIAAA, Div Biometry & Epidemiol, Suite 514,6000 Execut Blvd,MSC-7003, Rockville, MD 20852 USA. NR 20 TC 118 Z9 120 U1 1 U2 5 PU ABLEX PUBL CORP PI STAMFORD PA 100 PROSPECT ST, PO BOX 811, STAMFORD, CT 06904-0811 USA SN 0899-3289 J9 J SUBST ABUSE JI J. SUBST. ABUSE PY 1998 VL 10 IS 1 BP 59 EP 73 DI 10.1016/S0899-3289(99)80141-2 PG 15 WC Substance Abuse SC Substance Abuse GA 110QV UT WOS:000075392600007 PM 9720007 ER PT J AU Dawson, DA AF Dawson, DA TI Symptoms and characteristics of individuals with different types of recovery from DSM-IV alcohol dependence SO JOURNAL OF SUBSTANCE ABUSE LA English DT Article ID FOLLOW-UP; CONTROLLED DRINKING; PROBLEM DRINKERS; UNITED-STATES; NATURAL-HISTORY; NON-ABSTINENT; AGE; PREDICTORS; PREVALENCE; SEVERITY AB Symptoms and criteria for DSM-IV alcohol dependence and demographic and drinking characteristics are compared for three groups Group I (N = 1,044) consists of persons who formerly met the criteria for dependence but were abstinent in the past year, Group II (N = 2,325) consists of persons who were formerly dependent but did nor meet the criteria for abuse or dependence in the past year despite drinking, and Group III (N = 22,204) consists of persons who never met the criteria for dependence. Members of Group II lay between members of Groups I and III in terms of the numbers of prior dependence symptoms and criteria, past level of intake, degree of familial alcoholism and history of alcohol treatment Both groups of former alcoholics were equally likely to have experienced withdrawal, drinking more/longer than intended and tolerance. The criteria that most strongly distinguished the groups were continued use despite physical of psychological consequences, time spent drinking and activities given up, all of which were far more common in Group I than in Group II. Members of Group II had earlier onsets of heavy drinking and dependence than members of Group I, supporting the existence of a developmentally limited subtype of alcoholism that is subject to spontaneous remission in early adulthood without treatment. The paper compares three options for tightening the criteria for dependence, all of which remove more members from Group II (28% to 41% depending on option) than from Group I (12% to 19%). C1 NIAAA, DBE, NIH, Bethesda, MD 20892 USA. RP Dawson, DA (reprint author), NIAAA, DBE, NIH, Willco Bldg,Suite 514,6000 Execut Blvd MSC 7003, Bethesda, MD 20892 USA. NR 54 TC 9 Z9 9 U1 2 U2 3 PU ABLEX PUBL CORP PI STAMFORD PA 100 PROSPECT ST, PO BOX 811, STAMFORD, CT 06904-0811 USA SN 0899-3289 J9 J SUBST ABUSE JI J. SUBST. ABUSE PY 1998 VL 10 IS 2 BP 127 EP 142 DI 10.1016/S0899-3289(99)80129-1 PG 16 WC Substance Abuse SC Substance Abuse GA 145GG UT WOS:000077362200003 PM 9854699 ER PT J AU Grant, BF Dawson, DA AF Grant, BF Dawson, DA TI Age of onset of drug use and its association with DSM-IV drug abuse and dependence: Results from the National Longitudinal Alcohol Epidemiologic Survey SO JOURNAL OF SUBSTANCE ABUSE LA English DT Article ID RELIABILITY; SAMPLE AB The purpose of this study was to examine the relationship between early onset drug use and the development of lifetime DSM-IV drug abuse and dependence using a representative sample of the U.S. population. Prevalences of lifetime drug abuse and dependence were estimated for each year of age of onset of drug use from ages 13 and younger to 21 and older for the overall sample of drug users by race and gender. Linear logistic analyses were conducted to assess the relationship between age of drug use onset and lifetime drug use disorders controlling for important covariates. The major finding of this study was that early onset drug use is a significant predictor of the subsequent development of drug abuse over the life course. Early onset drug use was also a significant predictor of the subsequent development of lifetime alcohol dependence among males, females, and nonblacks, but not among blacks. After adjusting for important model covariates, the likelihood of lifetime drug abuse and dependence among the total sample of lifetime drug users was reduced by 4% and 5% with each year drug use onset was delayed. Implications of these findings are discussed in terms of the importance of collecting national data on drug use, abuse and dependence and the need for further research and its integration with prevention efforts. C1 NIAAA, Div Biometry & Epidemiol, Rockville, MD 20852 USA. RP Grant, BF (reprint author), NIAAA, Div Biometry & Epidemiol, Suite 514,6000 Execut Blvd,MSC-7003, Rockville, MD 20852 USA. NR 25 TC 280 Z9 284 U1 2 U2 10 PU ABLEX PUBL CORP PI STAMFORD PA 100 PROSPECT ST, PO BOX 811, STAMFORD, CT 06904-0811 USA SN 0899-3289 J9 J SUBST ABUSE JI J. SUBST. ABUSE PY 1998 VL 10 IS 2 BP 163 EP 173 DI 10.1016/S0899-3289(99)80131-X PG 11 WC Substance Abuse SC Substance Abuse GA 145GG UT WOS:000077362200005 PM 9854701 ER PT J AU Grant, BF Pickering, R AF Grant, BF Pickering, R TI The relationship between cannabis use and DSM-IV cannabis abuse and dependence: Results from the national longitudinal alcohol epidemiologic survey SO JOURNAL OF SUBSTANCE ABUSE LA English DT Article ID COMORBIDITY-SURVEY; UNITED-STATES; PREVALENCE AB The purpose of this study was to determine the risk of Diagnostic and Statistical Manual of Mental Disorders-Fourth Edition (DSM-IV) cannabis abuse and dependence at different levels of cannabis use in a nationally representative sample of the U.S. general population. Two separate logistic regression analyses were conducted to determine the association between cannabis use, and abuse and dependence. The risk of cannabis abuse and dependence was found to ina ease with the frequency of smoking occasions and slightly decreased with age. More severe comorbidity was associated with dependence compared to abuse, suggesting that cannabis might be used to self-medicate major depression. The strength of the association between cannabis use and abuse was also increased as a function of the number of joints smoked among females, but not males. These results were discussed in terms of differential societal reactions, the self-medication hypothesis, and gender biases in diagnosing cannabis abuse. C1 NIAAA, Div Biometry & Epidemiol, Bethesda, MD 20892 USA. RP Grant, BF (reprint author), NIAAA, Div Biometry & Epidemiol, Suite 514,6000 Execut Blvd,MSC 7003, Bethesda, MD 20892 USA. NR 14 TC 64 Z9 64 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0899-3289 J9 J SUBST ABUSE JI J. SUBST. ABUSE PY 1998 VL 10 IS 3 BP 255 EP 264 DI 10.1016/S0899-3289(99)00006-1 PG 10 WC Substance Abuse SC Substance Abuse GA 263FM UT WOS:000084114800003 PM 10689658 ER PT J AU Dawson, DA AF Dawson, DA TI Beyond black, white and Hispanic: Race, ethnic origin and drinking patterns in the United States SO JOURNAL OF SUBSTANCE ABUSE LA English DT Article ID ALCOHOL-USE AB This study used data on 42,862 U.S. adults, including 18,352 past-year drinkers, to describe differentials by race and national origin in U.S. drinking patterns. Age-sex standardized estimates were presented within 21 categories of ethnic origin for whites and within five categories each for individuals of black and other races. Of the three racial groups, whites were the most likely to drink but blacks had the highest volume of intake and frequency of heavy drinking. Differences by ethnic origin within racial categories were as marked as differentials between races. Compared to whites of European origin, those of Hispanic and native American origin were less likely to drink but consumed more alcohol on days when they drank. Whites of Southern and Eastern European origin drank proportionately more wine and demonstrated more moderate drinking patterns (lower intake per drinking day and/or less frequent heavy drinking) than those of Northern or Central European origin. Hispanics of Caribbean origin were less prone to heavy drinking than other white Hispanics; similarly, blacks from the English-speaking Caribbean showed more moderate drinking patterns than other blacks. Individuals of Asian origin, in particular those of non-Japanese origin, had the most moderate drinking patterns within the category of other race. Although the black/white differentials in volume of intake and frequency of heavy drinking disappeared after adjusting for marital status, education and income, most of the differences by ethnic origin retained their statistical significance if not their original magnitudes. These findings indicate that cultural forces exert a strong effect on drinking behavior Differences among European whites with respect to prevalence of drinking, beverage preference and frequency of heavy drinking suggest that the association between ethnic origin and drinking behavior may persist even after many generations of presumed acculturation. C1 NIAAA, DBE, NIH, Bethesda, MD 20892 USA. RP Dawson, DA (reprint author), NIAAA, DBE, NIH, 6000 Execut Blvd,MSC 7003, Bethesda, MD 20892 USA. NR 39 TC 85 Z9 85 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0899-3289 J9 J SUBST ABUSE JI J. SUBST. ABUSE PY 1998 VL 10 IS 4 BP 321 EP 339 DI 10.1016/S0899-3289(99)00009-7 PG 19 WC Substance Abuse SC Substance Abuse GA 262AN UT WOS:000084043700001 PM 10897287 ER PT J AU Rumsey, JM AF Rumsey, JM TI Brain imaging of reading disorders SO JOURNAL OF THE AMERICAN ACADEMY OF CHILD AND ADOLESCENT PSYCHIATRY LA English DT Letter ID DYSLEXIA C1 NIMH, Bethesda, MD 20892 USA. RP Rumsey, JM (reprint author), NIMH, Bethesda, MD 20892 USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0890-8567 J9 J AM ACAD CHILD PSY JI J. Am. Acad. Child Adolesc. Psychiatr. PD JAN PY 1998 VL 37 IS 1 BP 12 EP 12 DI 10.1097/00004583-199801000-00007 PG 1 WC Psychology, Developmental; Pediatrics; Psychiatry SC Psychology; Pediatrics; Psychiatry GA YW319 UT WOS:000071922000006 PM 9444890 ER PT J AU Kumra, S Jacobsen, LK Lenane, M Zahn, TP Wiggs, E Alaghband-Rad, J Castellanos, FX Frazier, JA McKenna, K Gordon, CT Smith, A Hamburger, S Rapoport, JL AF Kumra, S Jacobsen, LK Lenane, M Zahn, TP Wiggs, E Alaghband-Rad, J Castellanos, FX Frazier, JA McKenna, K Gordon, CT Smith, A Hamburger, S Rapoport, JL TI "Multidimensionally impaired disorder": Is it a variant of very early-onset schizophrenia? SO JOURNAL OF THE AMERICAN ACADEMY OF CHILD AND ADOLESCENT PSYCHIATRY LA English DT Article DE children; atypical psychosis; multidimensionally impaired; very early-onset schizophrenia ID DEFICIT HYPERACTIVITY DISORDER; DISRUPTIVE BEHAVIOR DISORDERS; CHILDHOOD SCHIZOPHRENIA; DEVELOPMENTAL DISORDERS; DSM-IV; CHILDREN; PSYCHOSES; CLASSIFICATION; COMORBIDITY; PERSONALITY AB Objective: To examine the validity of diagnostic criteria for a subgroup of children with atypical psychosis (n = 19), designated here as "multidimensionally impaired." These children are characterized by poor attention and impulse control, psychotic symptoms, and poor affective control. Method: Children and adolescents (n = 19) meeting our criteria for multidimensionally impaired syndrome with onset of psychotic symptoms at or before age 12 years were identified from a total of 150 in-person screenings for very early-onset schizophrenia between 1990 and 1996. We compared the premorbid adjustment, family history, follow-up status, and laboratory measures for a subgroup of these children with those of (1) a rigorously defined group of 29 children with DSM-III-R schizophrenia and (2) 19 children with attention-deficit hyperactivity disorder. Results: Patients with multidimensionally impaired syndrome and patients with very early-onset schizophrenia shared a similar pattern of early transient autistic features, postpsychotic cognitive decline, and an elevated risk of schizophrenic-spectrum disorders among their first-degree relatives. This pattern was not seen in the attention-deficit hyperactivity disorder group. In contrast to very early-onset schizophrenia, the multidimensionally impaired group had significantly poorer scores on the Freedom From Distractibility factor on the WISC-R, a less deviant pattern of autonomic reactivity, and no progression to schizophrenia. Conclusions: The findings support the distinction of the multidimensionally impaired cases as separate from those with other psychiatric disorders, and there is somewhat greater evidence to suggest that this disorder belongs in the schizophrenia spectrum. C1 NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. Harvard Univ, Sch Med, Boston, MA USA. Univ Maryland, Sch Med, Baltimore, MD 21201 USA. Northwestern Univ, Sch Med, Chicago, IL USA. NIMH, Psychol & Psychopathol Lab, Bethesda, MD 20892 USA. RP Kumra, S (reprint author), NIMH, Child Psychiat Branch, Bldg 10,Room 6N240,10 Ctr Dr MSC1600, Bethesda, MD 20892 USA. NR 56 TC 74 Z9 75 U1 1 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0890-8567 J9 J AM ACAD CHILD PSY JI J. Am. Acad. Child Adolesc. Psychiatr. PD JAN PY 1998 VL 37 IS 1 BP 91 EP 99 DI 10.1097/00004583-199801000-00022 PG 9 WC Psychology, Developmental; Pediatrics; Psychiatry SC Psychology; Pediatrics; Psychiatry GA YW319 UT WOS:000071922000021 PM 9444905 ER PT J AU Selwitz, RH AF Selwitz, RH TI Placing health promotion into the context of our lives SO JOURNAL OF THE AMERICAN DENTAL ASSOCIATION LA English DT Article C1 NIDR, Bethesda, MD 20892 USA. RP Selwitz, RH (reprint author), NIDR, 31 Ctr Dr,MSC 2290,Bldg 31,Room 2C39, Bethesda, MD 20892 USA. NR 3 TC 0 Z9 1 U1 0 U2 0 PU AMER DENTAL ASSN PI CHICAGO PA 211 E CHICAGO AVE, CHICAGO, IL 60611 USA SN 0002-8177 J9 J AM DENT ASSOC JI J. Am. Dent. Assoc. PD JAN PY 1998 VL 129 IS 1 BP 91 EP 95 PG 5 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA YQ211 UT WOS:000071361700014 ER PT J AU Reimers, TM Brown, KM Van Horn, L Stevens, V Obarzanek, E Hartmuller, VW Snetselaar, L Von Almen, TK Chiostri, J AF Reimers, TM Brown, KM Van Horn, L Stevens, V Obarzanek, E Hartmuller, VW Snetselaar, L Von Almen, TK Chiostri, J TI Maternal acceptability of a dietary intervention designed to lower children's intake of saturated fat and cholesterol: The Dietary Intervention Study in Children (DISC) SO JOURNAL OF THE AMERICAN DIETETIC ASSOCIATION LA English DT Article AB Objective This report examined the acceptability to mothers of a dietary educational and behavioral intervention for preadolescent children with elevated levels of serum low-density lipoprotein cholesterol (LDL-C) who were enrolled in the Dietary Intervention Study in Children (DISC). Design DISC is a randomized, controlled clinical trial. Subjects were randomly assigned to either an intervention or usual-care (control) group. Subjects/setting To be eligible for the study, participants were required to have the average of 2 fasting LDL-C values fall between the 80th and 98th sex-specific percentiles. Three hundred thirty-four 8- to 10-year-old children and their families were randomly assigned to an intervention group, and 329 were assigned to a usual-care (control) group. This study examined data from 232 subjects in the intervention group. Data were collected at 6 intervention sites around the United States. Intervention Those assigned to the intervention group participated in a multidisciplinary dietary intervention that included a series of group and individual sessions over a 3-year period. Children and their caretakers were taught to follow a nutritionally adequate diet that was low in total fat, saturated fat, and cholesterol and high in polyunsaturated fat. Main outcome measures Three nonconsecutive 24-hour diet recalls were collected at baseline and at 1 year by trained and certified dietitians. A questionnaire designed to assess diet acceptability was administered at months 4, 8, 11, and 15. Demographic measures were collected at the onset of the study. Statistical analysis performed Statistical procedures included factor analysis and regression analysis. Results Regression analysis suggested that perceived effectiveness of the dietary intervention and mothers' having few concerns about disadvantages of the diet were significantly related to higher overall fat intake in children in one-parent families. Maternal willingness to implement the diet was significantly related to lower saturated fat intake. Applications/conclusions In attempts to change eating behavior of children, interest and cooperation of the parents are essential to achieving successful results. These analyses further suggest that maternal acceptability translates into willingness to implement the diet and may facilitate changes that are associated with reduced saturated fat intake in children. C1 Childrens Hosp Omaha, Omaha, NE 68114 USA. Maryland Med Res Inst, Baltimore, MD USA. Northwestern Univ, Sch Med, Chicago, IL USA. Kaiser Permanente Ctr Hlth Res, Portland, OR USA. NHLBI, Bethesda, MD 20892 USA. Johns Hopkins Univ, Baltimore, MD USA. Univ Iowa, Iowa City, IA USA. Childrens Hosp, New Orleans, LA USA. RP Reimers, TM (reprint author), Childrens Hosp Omaha, Childrens Healthcare Pavil,8301 Dodge St, Omaha, NE 68114 USA. NR 17 TC 12 Z9 12 U1 0 U2 1 PU AMER DIETETIC ASSOC PI CHICAGO PA 216 W JACKSON BLVD #800, CHICAGO, IL 60606-6995 USA SN 0002-8223 J9 J AM DIET ASSOC JI J. Am. Diet. Assoc. PD JAN PY 1998 VL 98 IS 1 BP 31 EP 34 DI 10.1016/S0002-8223(98)00010-8 PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA YP592 UT WOS:000071293500008 PM 9434647 ER PT J AU Leontos, C Wong, F Gallivan, J Lising, M AF Leontos, C Wong, F Gallivan, J Lising, M CA Natl Diabet Educ Program Strategic Planning Co TI National Diabetes Education Program: Opportunities and challenges SO JOURNAL OF THE AMERICAN DIETETIC ASSOCIATION LA English DT Article ID MICROVASCULAR COMPLICATIONS; MELLITUS AB The National Diabetes Education Program is a joint initiative of the National Institutes of Health and the Centers for Disease Control and Prevention. This article outlines the program, points out implications for dietetics professionals, and offers suggestions as to how they may become involved. C1 NIDDK, NIH, Bethesda, MD USA. Ctr Dis Control & Prevent, Div Diabet Translat, NDEP, Atlanta, GA USA. RP Leontos, C (reprint author), Univ Nevada, Cooperat Extens, 2345 Red Rock St, Las Vegas, NV 89102 USA. NR 19 TC 8 Z9 8 U1 0 U2 0 PU AMER DIETETIC ASSOC PI CHICAGO PA 216 W JACKSON BLVD #800, CHICAGO, IL 60606-6995 USA SN 0002-8223 J9 J AM DIET ASSOC JI J. Am. Diet. Assoc. PD JAN PY 1998 VL 98 IS 1 BP 73 EP 75 DI 10.1016/S0002-8223(98)00019-4 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA YP592 UT WOS:000071293500016 PM 9434654 ER PT J AU Humphreys, BL Lindberg, DAB Schoolman, HM Barnett, GO AF Humphreys, BL Lindberg, DAB Schoolman, HM Barnett, GO TI The Unified Medical Language System: An informatics research collaboration SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Article ID WORLD-WIDE-WEB AB In 1986, the National Library of Medicine (NLM) assembled a large multidisciplinary, multisite team to work on the Unified Medical Language System (UMLS), a collaborative research project aimed at reducing fundamental barriers to the application of computers to medicine. Beyond its tangible products, the UMLS Knowledge Sources, and its influence on the field of informatics, the UMLS project is an interesting case study in collaborative research and development. It illustrates the strengths and challenges of substantive collaboration among widely distributed research groups. Over the past decade, advances in computing and communications have minimized the technical difficulties associated with UMLS collaboration and also facilitated the development, dissemination, and use of the UMLS Knowledge Sources. The spread of the World Wide Web has increased the visibility of the information access problems caused by multiple vocabularies and many information sources which are the focus of UMLS work. The time is propitious for building on UMLS accomplishments and making more progress on the informatics research issues first highlighted by the UMLS project more than 10 years ago. C1 Natl Lib Med, Bethesda, MD 20894 USA. Massachusetts Gen Hosp, Comp Sci Lab, Boston, MA 02114 USA. RP Humphreys, BL (reprint author), Natl Lib Med, 8600 Rockville Pike, Bethesda, MD 20894 USA. EM blh@nlm.nih.gov NR 30 TC 230 Z9 231 U1 1 U2 10 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PD JAN-FEB PY 1998 VL 5 IS 1 BP 1 EP 11 PG 11 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA YR037 UT WOS:000071451000001 PM 9452981 ER PT J AU McCray, AT Miller, RA AF McCray, AT Miller, RA TI Making the conceptual connections: The UMLS after a decade of research and development SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Editorial Material C1 Natl Lib Med, Bethesda, MD 20894 USA. Vanderbilt Univ, Med Ctr, Nashville, TN USA. RP McCray, AT (reprint author), Natl Lib Med, Bldg 38A,Room BIN-28H, Bethesda, MD 20894 USA. NR 10 TC 13 Z9 13 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PD JAN-FEB PY 1998 VL 5 IS 1 BP 129 EP 130 PG 2 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA YR037 UT WOS:000071451000012 PM 9471340 ER PT J AU Lindberg, DAB Siegel, ER AF Lindberg, DAB Siegel, ER TI G7: A framework for international cooperation in medical informatics SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Article AB The world's major economic powers, the G7, have initiated a collaborative International research and demonstration program to exploit the benefits of information and communications technology for society. The Global Healthcare Applications Project (GHAP) is investigating a variety of informatics applications in disease specific domains, telemedicine, and multilingual textual and image database systems. This paper summarizes the nine GHAP sub-projects undertaken to date, with emphasis on those in which the U.S. is a participant. The growing use of smart card technology, especially in Europe, is adding new impetus for similar medical and health experiments in the U.S. A pilot project now underway in several Western states is described. C1 Natl Lib Med, Hlth Informat Programs Dev, Bethesda, MD 20894 USA. RP Lindberg, DAB (reprint author), Natl Lib Med, Hlth Informat Programs Dev, Bethesda, MD 20894 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 15 EP 18 PG 4 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600004 ER PT J AU Sneiderman, CA Rindflesch, TC Bean, CA AF Sneiderman, CA Rindflesch, TC Bean, CA TI Identification of anatomical terminology in medical text SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Article AB We report on an experiment to use the natural language processing tools being developed in the SPECIALIST(TM) system to accurately identify terminology associated with the coronary arteries as expressed in coronary catheterization reports. The ultimate goal is to map from any anatomically-oriented medical text to online images, using the UMLS(R) as an intermediate knowledge source. We describe some of the problems encountered when processing coronary artery terminology and report on the results of a formative evaluation of a tool for addressing these problems. C1 Natl Lib Med, Bethesda, MD 20894 USA. RP Sneiderman, CA (reprint author), Natl Lib Med, Bethesda, MD 20894 USA. NR 13 TC 1 Z9 1 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 428 EP 432 PG 5 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600082 ER PT J AU Divita, G Browne, AC Rindflesch, TC AF Divita, G Browne, AC Rindflesch, TC TI Evaluating lexical variant generation to improve information retrieval SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Article AB Techniques for managing lexical variation constitute an integral part of information retrieval systems. We report on a series of experiments aimed at evaluating LVG, a lexical variant management tool which addresses the particular problems involved in matching health related vocabularies to concepts in the Unified Medical Language System(R) (UMLS(R)) Metathesaurus(R) Experiments conducted on data from the Large Scale Vocabulary Test indicate the effectiveness of this approach to managing biomedical information. C1 Management Syst Designers, Vienna, VA 22180 USA. Natl Lib Med, Bethesda, MD 20894 USA. RP Divita, G (reprint author), Management Syst Designers, Vienna, VA 22180 USA. NR 15 TC 4 Z9 4 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 775 EP 779 PG 5 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600151 ER PT J AU McCray, AT Browne, AC AF McCray, AT Browne, AC TI Discovering the modifiers in a terminology data set SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Article AB We recently conducted a study of a subset of the data collected in the NLM/AHCPR Large Scale Vocabulary Test (LSVT). We studied those 11,387 terms in the LSVT data that were narrower in meaning than the UMLS concepts mapped to. We hypothesized that when one term is narrower in meaning than another, the first and second terms are likely to differ primarily by modification. We compared three lexical processing methods of increasing sophistication and measured the ability of each of these methods to correctly identify the modifiers in the data set. The results indicate that when using the most powerful of the methods, 63% of the term pairs were found to differ only by premodification, by postmodification or by both, 31% share some lexical material, and the remaining 6% have no lexical items in common. The implications of the study are discussed. C1 Natl Lib Med, Bethesda, MD 20209 USA. RP McCray, AT (reprint author), Natl Lib Med, Bethesda, MD 20209 USA. NR 9 TC 0 Z9 0 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 780 EP 784 PG 5 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600152 ER PT J AU Bodenreider, O Nelson, SJ Hole, WT Chang, HF AF Bodenreider, O Nelson, SJ Hole, WT Chang, HF TI Beyond synonymy: Exploiting the UMLS semantics in mapping vocabularies SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Article AB The Unified Medical Language System (UMLS) contains semantic information about terms from various sources, each concept can be understood and located by its relationships to other concepts: this is a result of the organizing principle of semantic locality. We describe a method in which the semantic relationships between concepts are used to map concepts from different vocabularies in the UMLS. Applied to mapping concepts to MeSH, this method is able to map 50 to 65% of the non-MeSH concepts to MeSH. A manual review of the mapping shows a relevance rate of 61%. Causes of failure include a lack of consistently represented relationships in the UMLS, and some inconsistencies in the categorization of the concepts. The limits of this method are discussed, as well as possible adaptations for other uses. C1 Management Syst Designers, Vienna, VA 22180 USA. Natl Lib Med, Bethesda, MD USA. RP Bodenreider, O (reprint author), Management Syst Designers, Vienna, VA 22180 USA. NR 11 TC 14 Z9 14 U1 0 U2 1 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 815 EP 819 PG 5 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600159 ER PT J AU Bean, CA Rindflesch, TC Sneiderman, CA AF Bean, CA Rindflesch, TC Sneiderman, CA TI Automatic semantic interpretation of anatomic spatial relationships in clinical text SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Article AB A set of semantic interpretation rules to link the syntax and semantics of locative relationships among anatomic entities was developed and implemented in a natural language processing system. Two experiments assessed the ability of the system to identify and characterize physico-spatial relationships in coronary angiography reports. Branching relationships were by far the most common observed (75%), followed by PATH (20%) and PART/WHOLE relationships. Recall and precision scores were 0.78 and 0.67 overall, suggesting the viability of this approach in semantic processing of clinical text. C1 Natl Lib Med, Bethesda, MD 20894 USA. RP Bean, CA (reprint author), Natl Lib Med, Bethesda, MD 20894 USA. NR 11 TC 0 Z9 0 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 897 EP 901 PG 5 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600175 ER PT J AU Browne, AC Divita, G Nguyen, V Cheng, VC AF Browne, AC Divita, G Nguyen, V Cheng, VC TI Modular text processing system based on the SPECIALIST lexicon and lexical tools SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Meeting Abstract C1 Natl Lib Med, Bethesda, MD 20894 USA. Management Syst Designers, Vienna, VA 22180 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 982 EP 982 PG 1 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600206 ER PT J AU Johnston, D Nelson, SJ Schulman, JLA Savage, AG Powell, TP AF Johnston, D Nelson, SJ Schulman, JLA Savage, AG Powell, TP TI Redefining a thesaurus: Term-centric no more SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Meeting Abstract C1 Natl Lib Med, Bethesda, MD USA. NR 4 TC 1 Z9 1 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 1025 EP 1025 PG 1 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600249 ER PT J AU Nelson, SJ Kuhn, T Radzinski, D Sherertz, DD Tuttle, MS Spena, R AF Nelson, SJ Kuhn, T Radzinski, D Sherertz, DD Tuttle, MS Spena, R TI Creating a thesaurus from text: A "bottom-up" approach to organizing medical knowledge SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Meeting Abstract C1 Natl Lib Med, Bethesda, MD USA. Amer Coll Physicians, Philadelphia, PA USA. Lex Technol Inc, Alameda, CA USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 1046 EP 1046 PG 1 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600270 ER PT J AU Wu, CH Srinivasan, S Nelson, SJ AF Wu, CH Srinivasan, S Nelson, SJ TI An experience in merging thesauri: Using terms from other thesauri to enhance MeSH SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION LA English DT Meeting Abstract AB This study examined whether terms from the Unified Medical Language System related to the Medical Subject Headings (MeSH) concepts by certain relationships were useful as entry terms to MeSH. C1 NYU, Sch Med, Ehrman Med Lib, New York, NY USA. Management Syst Designers, Vienna, Austria. Natl Lib Med, Bethesda, MD USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 USA SN 1067-5027 J9 J AM MED INFORM ASSN JI J. Am. Med. Inf. Assoc. PY 1998 SU S BP 1101 EP 1101 PG 1 WC Computer Science, Information Systems; Computer Science, Interdisciplinary Applications; Information Science & Library Science; Medical Informatics SC Computer Science; Information Science & Library Science; Medical Informatics GA V3156 UT WOS:000171768600325 ER PT J AU Zhang, XK Fales, HM AF Zhang, XK Fales, HM TI Electrospray mass spectrum of a Per(onio)-substituted benzene: Retention of coulombic charge upon collisionally activated decomposition SO JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY LA English DT Article ID DISSOCIATION; IONS AB The hexakis (4-dimethylaminopyridyl) benzene hexacation of 1 is investigated as an extreme example of the ability of electrospray ionization to allow transfer of small multivalent ions to the gas phase. The hexacationized benzene ring ions are stabilized by forming gas phase complexes with two to five trifluoromethanesulfonate counterions. MS/MS analysis reveals that their fragmentation takes place by loss of neutrals such as trifluoromethanesulfonic acid and 4-dimethylaminopyridine; no rupture of the benzene or pyridine rings was observed in spite of accumulation of positive charge in a restricted geometry. C1 NHLBI, Biophys Chem Lab, NIH, Ctr Clin, Bethesda, MD 20892 USA. RP Fales, HM (reprint author), NHLBI, Biophys Chem Lab, NIH, Ctr Clin, Bldg 10,Rm 7N318,10 Ctr Dr MSC 1676, Bethesda, MD 20892 USA. NR 6 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 1044-0305 J9 J AM SOC MASS SPECTR JI J. Am. Soc. Mass Spectrom. PD JAN PY 1998 VL 9 IS 1 BP 15 EP 20 DI 10.1016/S1044-0305(97)00233-X PG 6 WC Chemistry, Analytical; Chemistry, Physical; Spectroscopy SC Chemistry; Spectroscopy GA YN227 UT WOS:000071145800003 PM 9679592 ER PT J AU Teigen, PM AF Teigen, PM TI Osler: Inspirations from a great physician. SO JOURNAL OF THE HISTORY OF MEDICINE AND ALLIED SCIENCES LA English DT Book Review C1 Natl Lib Med, Hist Med Div, Bethesda, MD 20894 USA. RP Teigen, PM (reprint author), Natl Lib Med, Hist Med Div, Bethesda, MD 20894 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-5045 J9 J HIST MED ALL SCI JI J. Hist. Med. Allied Sci. PD JAN PY 1998 VL 53 IS 1 BP 87 EP 88 PG 2 WC Health Care Sciences & Services; History & Philosophy Of Science SC Health Care Sciences & Services; History & Philosophy of Science GA ZQ196 UT WOS:000073832200007 ER PT J AU Kulldorff, M AF Kulldorff, M TI Untitled SO JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES A-STATISTICS IN SOCIETY LA English DT Letter ID SCAN STATISTICS; LEUKEMIA; CLUSTERS; DISEASE C1 NCI, Biometry Branch, Bethesda, MD 20892 USA. RP Kulldorff, M (reprint author), NCI, Biometry Branch, 6130 Execut Blvd, Bethesda, MD 20892 USA. RI Kulldorff, Martin/H-4282-2011 NR 10 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBL LTD PI OXFORD PA 108 COWLEY RD, OXFORD OX4 1JF, OXON, ENGLAND SN 0964-1998 J9 J ROY STAT SOC A STA JI J. R. Stat. Soc. Ser. A-Stat. Soc. PY 1998 VL 161 BP 273 EP 273 DI 10.1111/1467-985X.00105 PN 2 PG 1 WC Social Sciences, Mathematical Methods; Statistics & Probability SC Mathematical Methods In Social Sciences; Mathematics GA ZY981 UT WOS:000074683000014 ER PT J AU Chambers, RL Sugden, RA Longford, N Little, RJ Binder, DA Dorfman, AH Brewer, K Cohen, MP Eltinge, JL Ghosh, M Maiti, T Haslett, S Hesterberg, TC Korn, EI Graubard, BI Nadarajah, S Rao, JNK Roberts, GR Robins, JM Rotnitzky, A Firth, D Bennett, KE Pfeffermann, D Skinner, CJ Holmes, DJ Goldstein, H Rasbash, J AF Chambers, RL Sugden, RA Longford, N Little, RJ Binder, DA Dorfman, AH Brewer, K Cohen, MP Eltinge, JL Ghosh, M Maiti, T Haslett, S Hesterberg, TC Korn, EI Graubard, BI Nadarajah, S Rao, JNK Roberts, GR Robins, JM Rotnitzky, A Firth, D Bennett, KE Pfeffermann, D Skinner, CJ Holmes, DJ Goldstein, H Rasbash, J TI Weighting for unequal selection probabilities in multilevel models - Discussion on the papers by Firth and Bennett and Pfeffermann et al. SO JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY LA English DT Editorial Material ID BOOTSTRAP; ESTIMATORS; PARAMETERS C1 Univ Southampton, Southampton SO9 5NH, Hants, England. Univ London Goldsmiths Coll, London SE14 6NW, England. De Montfort Univ, Leicester LE1 9BH, Leics, England. Univ Michigan, Ann Arbor, MI 48109 USA. STAT Canada, Ottawa, ON, Canada. US Bur Labor Stat, Washington, DC 20212 USA. Australian Natl Univ, Canberra, ACT, Australia. Natl Ctr Educ Stat, Washington, DC USA. Texas A&M Univ, College Stn, TX USA. Univ Florida, Gainesville, FL USA. Massey Univ, Palmerston North, New Zealand. MathSoft, Seattle, WA USA. NCI, Bethesda, MD 20892 USA. Univ Plymouth, Plymouth, Devon, England. Harvard Univ, Sch Publ Hlth, Cambridge, MA 02138 USA. RP Chambers, RL (reprint author), Univ Southampton, Southampton SO9 5NH, Hants, England. NR 37 TC 1 Z9 1 U1 0 U2 2 PU BLACKWELL PUBL LTD PI OXFORD PA 108 COWLEY RD, OXFORD OX4 1JF, OXON, ENGLAND SN 1369-7412 J9 J ROY STAT SOC B JI J. R. Stat. Soc. Ser. B-Stat. Methodol. PY 1998 VL 60 BP 41 EP 56 PN 1 PG 16 WC Statistics & Probability SC Mathematics GA ZK922 UT WOS:000073377900004 ER PT J AU Nussenblatt, RB Bron, A Chambers, W McCulley, JP Pericoi, M Ubels, JL Edelhauser, HF AF Nussenblatt, RB Bron, A Chambers, W McCulley, JP Pericoi, M Ubels, JL Edelhauser, HF TI Ophthalmologic perspectives on eye irritation testing SO JOURNAL OF TOXICOLOGY-CUTANEOUS AND OCULAR TOXICOLOGY LA English DT Article ID BOVINE CORNEAL OPACITY; ASSAY C1 NEI, NIH, Bethesda, MD 20892 USA. Univ Oxford, Nuffield Lab Ophthalmol, Oxford OX2 6AW, England. US FDA, Washington, DC 20204 USA. Univ Texas, SW Med Sch, Dallas, TX 75230 USA. Peritesco, Paris, France. Calvin Coll, Grand Rapids, MI 49506 USA. Emory Univ, Atlanta, GA 30322 USA. RP Nussenblatt, RB (reprint author), Care of Porter L, Hlth & Environm Sci Inst, 1126 16th St, Washington, DC 20036 USA. NR 15 TC 18 Z9 18 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 0731-3829 J9 J TOXICOL-CUTAN OCUL JI J. Toxicol.-Cutan. Ocul. Toxicol. PY 1998 VL 17 IS 2-3 BP 103 EP 109 PG 7 WC Ophthalmology; Toxicology SC Ophthalmology; Toxicology GA 126BY UT WOS:000076273900002 ER PT J AU Bremner, JD Krystal, JH Putnam, FW Southwick, SM Marmar, C Charney, DS Mazure, CM AF Bremner, JD Krystal, JH Putnam, FW Southwick, SM Marmar, C Charney, DS Mazure, CM TI Measurement of dissociative states with the Clinician-Administered Dissociative States Scale (CADSS) SO JOURNAL OF TRAUMATIC STRESS LA English DT Article DE dissociation; trauma; PTSD; psychometrics ID POSTTRAUMATIC-STRESS-DISORDER; MULTIPLE PERSONALITY-DISORDER; SYMPTOMS; RELIABILITY; EXPERIENCES; INTERVIEW; VALIDITY; VETERANS AB The purpose of this study was to develop an instrument for the measurement of present-state dissociative symptoms, the Clinician Administered Dissociative States Scale (CADSS). Reported here are interrater reliability and internal consistency of the CADSS, validity as assessed by comparisons with other instruments for the assessment of dissociation, and sensitivity of the CADSS to discriminate patients with dissociative disorders from patients with other psychiatric disorders nad healthy subjects. lnitial analyses indicated good interrater reliability and construct validity for the CADSS. Scores opt the CADSS discriminated patients with dissociative disorders from the other groups. C1 Yale Psychiat Inst, New Haven, CT 06520 USA. W Haven VA Med Ctr, W Haven, CT USA. Natl Ctr Posttraumat Stress Disorder, Div Clin Neurosci, W Haven, CT 06516 USA. Yale Univ, Sch Med, Dept Psychiat, New Haven, CT 06520 USA. NIMH, Bethesda, MD 20892 USA. San Francisco VA Med Ctr, San Francisco, CA USA. Univ Calif San Francisco, Dept Psychiat, San Francisco, CA 94143 USA. RP Bremner, JD (reprint author), Yale Psychiat Inst, POB 208038,Yale Stn, New Haven, CT 06520 USA. RI Bremner, James/B-1632-2013 NR 33 TC 258 Z9 258 U1 2 U2 7 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0894-9867 J9 J TRAUMA STRESS JI J. Trauma Stress PD JAN PY 1998 VL 11 IS 1 BP 125 EP 136 DI 10.1023/A:1024465317902 PG 12 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA YU729 UT WOS:000071748400009 PM 9479681 ER PT J AU Albertsen, PC Nease, RF Potosky, AL AF Albertsen, PC Nease, RF Potosky, AL TI Assessment of patient preferences among men with prostate cancer SO JOURNAL OF UROLOGY LA English DT Article DE prostate; prostatic neoplasms; quality of life; questionnaires ID MANAGEMENT; OUTCOMES; HEALTH AB Purpose: We developed a self-administered paper based instrument to assess patient preferences quantified as utilities for common outcomes associated with the management of prostate cancer. Materials and Methods: A total of 50 patients was invited to test a self-administered paper based instrument designed to assess preferences for health outcomes associated with the management of localized prostate cancer. The 50 patients were selected from a group of 625 randomly identified men with prostate cancer who responded to a survey instrument designed to assess health related quality of life. The 50 patients selected for this pilot project were chosen because of the wide range of responses to the quality of life survey. Patient utilities were assessed for the 5 health states of overall quality of life, problems related to prostate cancer, and problems related to urinary, bowel and sexual dysfunction. Results: Patients were able to complete the assigned tasks. The self-administered instrument had high test-retest reliability. In addition results obtained from this instrument showed a correlation with results obtained from assessments using other instruments, including an analog scale, a computer based system known as U-Titer, a quality of life survey and the Health Utility Index:3. Conclusions: A self-administered paper based instrument can be used to assess patient utilities for health states associated with prostate cancer management. Results from the instrument tested appear to be reliable and valid, and are comparable to those obtained from other assessment techniques. A self-administered paper based instrument has distinct advantages when conducting large survey studies because it can be incorporated at relatively low cost. C1 WASHINGTON UNIV, SCH MED, DEPT INTERNAL MED, DIV GEN MED SCI, ST LOUIS, MO USA. NCI, APPL RES BRANCH, BETHESDA, MD 20892 USA. RP UNIV CONNECTICUT, CTR HLTH, DEPT SURG, DIV UROL, FARMINGTON, CT 06032 USA. FU NCI NIH HHS [N01-CN05226, N01-CN67005] NR 19 TC 64 Z9 64 U1 3 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-5347 EI 1527-3792 J9 J UROLOGY JI J. Urol. PD JAN PY 1998 VL 159 IS 1 BP 158 EP 163 DI 10.1016/S0022-5347(01)64043-6 PG 6 WC Urology & Nephrology SC Urology & Nephrology GA YK232 UT WOS:A1998YK23200050 PM 9400461 ER PT J AU Hu, XL Wolffe, EJ Weisberg, AS Carroll, LJ Moss, B AF Hu, XL Wolffe, EJ Weisberg, AS Carroll, LJ Moss, B TI Repression of the A8L gene, encoding the early transcription factor 82-kilodalton subunit, inhibits morphogenesis of vaccinia virions SO JOURNAL OF VIROLOGY LA English DT Article ID VIRUS EARLY TRANSCRIPTION; POLYMERASE-ASSOCIATED PROTEIN; RNA-POLYMERASE; POLY(A) POLYMERASE; EARLY PROMOTER; EXPRESSION; DNA; COMPLEX; PURIFICATION; REACTIVATION AB The vaccinia virus early transcription factor (VETF) is a DNA binding protein comprised of 70- and 82-kDa subunits encoded by the D6R and ASL genes, respectively. A previous investigation suggested a novel role for the 70-kDa subunit in the morphogenesis of vaccinia virus particles. The principal objectives of the present study were to determine if the 82-kDa subunit of VETF is also required for morphogenesis and, if so, whether the block occurs before or after the incorporation of the genome into the assembling virus particle. To address these and other questions, we constructed and characterized a conditionally lethal recombinant vaccinia virus in which the A8L gene is stringently repressed by the Escherichia coli [ac operator system. The amount of 82-kDa protein synthesized could be regulated by the amount of inducer: from undetectable to higher than normal levels. Virus replication, as determined by plaque formation or virus yield upon synchronous infection, was dependent on inducer. Nevertheless, de novo synthesis of the 82-kDa subunit was not required for viral early, intermediate, and late gene expression or DNA replication. Overexpression of the ASL gene alone, produced by high concentrations of inducer, inhibited viral late protein synthesis, whereas overexpression of the D6R gene alone or both VETF genes simultaneously had little inhibitory effect. Laser confocal fluorescence and quantitative electron microscopic analyses revealed that immature and DNA-containing intermediate stage particles accumulated in the absence of inducer, indicating that the A8L protein has a role in morphogenesis of the core and subsequent events. C1 NIAID,VIRAL DIS LAB,NIH,BETHESDA,MD 20892. NR 37 TC 20 Z9 20 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1998 VL 72 IS 1 BP 104 EP 112 PG 9 WC Virology SC Virology GA YL010 UT WOS:A1998YL01000012 PM 9420205 ER PT J AU Reinhart, TA Rogan, MJ Amedee, AM MurpheyCorb, M Rausch, DM Eiden, LE Haase, AT AF Reinhart, TA Rogan, MJ Amedee, AM MurpheyCorb, M Rausch, DM Eiden, LE Haase, AT TI Tracking members of the simian immunodeficiency virus deltaB670 quasispecies population in vivo at single-cell resolution SO JOURNAL OF VIROLOGY LA English DT Article ID INFECTED RHESUS-MONKEYS; BIOLOGICAL PHENOTYPE; GENETIC-VARIANTS; HIV-1 ENTRY; AIDS; TYPE-1; PROGRESSION; RECEPTOR; MACAQUES; CLONING AB Genetically distinct lentiviruses constitute a quasispecies population that can evolve in response to selective forces. To move beyond characterization of the population as a whole to the behavior of individual members, we devised an in situ hybridization approach that uses genotype-specific probes. We used probes that detect simian immunodeficiency viruses (SIV) that differ in sequence in the V1 region of the surface envelope glycoprotein (env) gene to investigate the replication and cellular tropisms of four viral variants in the tissues of infected rhesus macaques. We found that the V1 genotypic variants replicated in spatially defined patterns and to different extents at each anatomic site. The two variants that replicated most extensively in animals with AIDS were detected in both macrophages and T lymphocytes in tissues. By extension of this approach, it will be possible to investigate the role of individual lentiviruses in a quasispecies in pathogenesis and to evaluate the effects of antiviral or immunotherapeutic treatment on select members of a quasispecies. C1 UNIV MINNESOTA,SCH MED,DEPT MICROBIOL,MINNEAPOLIS,MN 55455. TULANE UNIV,DELTA REG PRIMATE RES CTR,COVINGTON,LA 70433. NIMH,OFF AIDS,ROCKVILLE,MD 20857. NIMH,CELL BIOL LAB,BETHESDA,MD 20854. OI Eiden, Lee/0000-0001-7524-944X NR 43 TC 21 Z9 21 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1998 VL 72 IS 1 BP 113 EP 120 PG 8 WC Virology SC Virology GA YL010 UT WOS:A1998YL01000013 PM 9420206 ER PT J AU Day, PM Roden, RBS Lowy, DR Schiller, JT AF Day, PM Roden, RBS Lowy, DR Schiller, JT TI The papillomavirus minor capsid protein, L2, induces localization of the major capsid protein, L1, and the viral transcription/replication protein, E2, to PML oncogenic domains SO JOURNAL OF VIROLOGY LA English DT Article ID ACUTE PROMYELOCYTIC LEUKEMIA; PML/RAR-ALPHA PROTEIN; VIRUS-LIKE PARTICLES; BOVINE PAPILLOMAVIRUS; RAR-ALPHA; NUCLEAR MATRIX; T(15-17) TRANSLOCATION; MONOCLONAL-ANTIBODIES; TRANSFORMED CELLS; GENE-PRODUCTS AB We have used immunofluorescent staining and confocal microscopy to examine the subcellular localization of structural and nonstructural bovine papillomavirus (BPV) proteins in cultured cells that produce infectious virions. When expressed separately, L1, the major capsid protein, showed a diffuse nuclear distribution while L2, the minor capsid protein, was found to localize to punctate nuclear regions identified as promonocytic leukemia protein (PML) oncogenic domains (PODs). Coexpression of L1 and L2 induced a relocation of L1 into the PODs, leading to the colocalization of L1 and L2. The effect of L2 expression on the distribution of the nonstructural viral proteins E1 and E2, which are required for maintenance of the genome and viral DNA synthesis, was also examined. The localization of the E1 protein was unaffected by L2 expression. However, the pattern of anti-E2 staining was dramatically altered in L2-expressing cells. Similar to L1, E2 was shifted from a dispersed nuclear locality into the PODs and colocalized with L2. The recruitment of full-length E2 by L2 occurred in the absence of other viral components. L2 was shown previously to be essential for the generation of infectious BPV. Our present results provide evidence for a role for L2 in the organization of virion components by recruiting them to a distinct nuclear domain. This L2-dependent colocalization probably serves as a mechanism to promote the assembly of papillomaviruses either by increasing the local concentration of virion constituents or by providing the physical architecture necessary for efficient packaging and assembly. The data also suggest a role for a nonstructural viral protein, E2, in virion assembly, specifically the recruitment of the viral genome to the sites of assembly, through its high-affinity interaction with specific sequences in the viral DNA. C1 NCI,CELLULAR ONCOL LAB,DIV BASIC SCI,NIH,BETHESDA,MD 20892. NR 60 TC 131 Z9 136 U1 0 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1998 VL 72 IS 1 BP 142 EP 150 PG 9 WC Virology SC Virology GA YL010 UT WOS:A1998YL01000016 PM 9420209 ER PT J AU Matano, T Shibata, R Siemon, C Connors, M Lane, HC Martin, MA AF Matano, T Shibata, R Siemon, C Connors, M Lane, HC Martin, MA TI Administration of an anti-CD8 monoclonal antibody interferes with the clearance of chimeric simian/human immunodeficiency virus during primary infections of rhesus macaques SO JOURNAL OF VIROLOGY LA English DT Article ID TOXIC LYMPHOCYTES-T; TYPE-1 INFECTION; HIV-1 INFECTION; CELLS; CD28; RESPONSES; DISEASE; MICE; MONKEYS; ACTIVATION AB Parenteral administration of a mouse anti-human CD8 monoclonal antibody (MAb) to rhesus macaques resulted in a transient depletion of CD8(+) cells in both the peripheral blood and lymphoid tissues. When administered during primary chimeric simian/human immunodeficiency virus infections, the CD8 MAb caused marked elevations of plasma and cell-associated virus levels in both the peripheral blood and lymphoid tissues and led to prolonged depletion of CD4 cells. Taken together, these results directly demonstrate that CD8(+) T lymphocytes are actively involved in controlling the acute phase of primate lentivirus infections. C1 NIAID,MOL MICROBIOL LAB,NIH,BETHESDA,MD 20892. NIAID,IMMUNOREGULAT LAB,NIH,BETHESDA,MD 20892. NR 35 TC 369 Z9 376 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1998 VL 72 IS 1 BP 164 EP 169 PG 6 WC Virology SC Virology GA YL010 UT WOS:A1998YL01000019 PM 9420212 ER PT J AU Glamann, J Burton, DR Parren, PWHI Ditzel, HJ Kent, KA Arnold, C Montefiori, D Hirsch, VM AF Glamann, J Burton, DR Parren, PWHI Ditzel, HJ Kent, KA Arnold, C Montefiori, D Hirsch, VM TI Simian immunodeficiency virus (SIV) envelope-specific Fabs with high-level homologous neutralizing activity: Recovery from a long-term-nonprogressor SIV-Infected macaque SO JOURNAL OF VIROLOGY LA English DT Article ID MONOCLONAL-ANTIBODIES; PASSIVE-IMMUNIZATION; IMMUNE-RESPONSES; HIV-1 INFECTION; RHESUS MACAQUES; TYPE-1; GLYCOPROTEIN; GP120; LIBRARIES; EPITOPES AB An antibody phage display library was constructed from RNA extracted from lymph node cells of a simian immunodeficiency virus (SIV)-infected long-term-nonprogressor macaque. Seven gp120-reactive Fabs were obtained by selection of the library against SIV monomeric gp120. Although each of the Fabs was unique in sequence, there were two distinct groups based on epitope recognition, neutralizing activity in vitro, and molecular analysis. Group 1 Fabs did not neutralize SIV and bound to a linear epitope in the V3 loop of the SIV envelope. In contrast, two of the group 2 Fabs neutralized homologous, neutralization-sensitive SIVsm isolates with high efficiency but failed to neutralize heterologous SIVmac isolates. Based on competition enzyme-linked immunosorbent assays with mouse monoclonal antibodies of known specificity, these Fabs reacted with a conformational epitope that includes domains V3 and V4 of the SIV envelope. These neutralizing and nonneutralizing Fabs provide valuable standardized and renewable reagents for studying the role of antibody in preventing or modifying SIV infection in vivo. C1 NIAID, TWINBROOK FACIL 2,IMMUNODEFICIENCY VIRUSES SECT, LAB INFECT DIS,NIH, ROCKVILLE, MD 20852 USA. Scripps Res Inst, DEPT IMMUNOL, LA JOLLA, CA 92037 USA. Scripps Res Inst, DEPT MOL BIOL, LA JOLLA, CA 92037 USA. DUKE UNIV, MED CTR, DEPT SURG, DURHAM, NC 27710 USA. NIBSC, POTTERS BAR, HERTS, ENGLAND. OI Ditzel, Henrik J./0000-0003-3927-5135; Parren, Paul/0000-0002-4365-3859 NR 53 TC 36 Z9 36 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1998 VL 72 IS 1 BP 585 EP 592 PG 8 WC Virology SC Virology GA YL010 UT WOS:A1998YL01000069 PM 9420262 ER PT J AU Ferran, MC McBride, AA AF Ferran, MC McBride, AA TI Transient viral DNA replication and repression of viral transcription are supported by the C-terminal domain of the bovine papillomavirus type 1 E1 protein SO JOURNAL OF VIROLOGY LA English DT Article ID E2 PROTEIN; COMPLEX-FORMATION; T-ANTIGEN; BINDING; ORIGIN; PHOSPHOPROTEIN; ACTIVATION; CONTAINS; TRANSACTIVATOR; IDENTIFICATION AB The bovine papillomavirus type 1 E1 protein is important for viral DNA replication and transcriptional repression. It has been proposed that the full-length E1 protein consists of a small N-terminal and a larger C-terminal domain. In this study, it is shown that an E1 polypeptide containing residues 132 to 605 (which represents the C-terminal domain) is able to support transient viral DNA replication, although at a level lower than that supported by the wild-type protein. This domain can also repress E-2-mediated transactivation from the P89 promoter as well as the wild-type E1 protein can. C1 NIAID,VIRAL DIS LAB,NIH,BETHESDA,MD 20892. OI McBride, Alison/0000-0001-5607-5157 NR 40 TC 20 Z9 21 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1998 VL 72 IS 1 BP 796 EP 801 PG 6 WC Virology SC Virology GA YL010 UT WOS:A1998YL01000096 PM 9420289 ER PT J AU Roubenoff, R Harris, TB Abad, LW Wilson, PWF Dallal, GE Dinarello, CA AF Roubenoff, R Harris, TB Abad, LW Wilson, PWF Dallal, GE Dinarello, CA TI Monocyte cytokine production in an elderly population: Effect of age and inflammation SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID TUMOR-NECROSIS-FACTOR; INTERLEUKIN-1 RECEPTOR ANTAGONIST; ERYTHROCYTE SEDIMENTATION-RATE; BLOOD MONONUCLEAR-CELLS; FACTOR-ALPHA; IL-6; MICE; PROTEIN; WOMEN; METABOLISM AB Objective. To determine the association among aging, inflammation, and cytokine production by peripheral blood mononuclear cells. Population and Methods. We examined production of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), IL-1 receptor antagonist (IL-1Ra), and IL-6 in 711 elderly participants in the Framingham Heart Study (mean age, 79 y) and 21 young healthy volunteers (mean age, 39 y). The elderly subjects were categorized by serum C-reactive protein (CRP) concentration, a marker of systemic inflammation. Results, Production of IL-6 (p<.00001) and IL-1Ra (p<.00001) was higher in the elderly subjects than in the control group. IL-6 production increased with increasing CRP, whereas IL-1RA was uniformly elevated in elderly subjects regardless of CRP. However, we found no difference in the production of IL-1 beta or TNF-alpha between the young and elderly groups, regardless of CRP status. IL-6 population correlated with IL-1 beta (r=.36, p<.0001) and TNF-alpha production (r=.25, p<.0001), but IL-1Ra production did not. Conclusion, Production of IL-6 and IL-1Ra - but not IL-1 beta or TNF-alpha - was increased in the elderly compared to healthy, young subjects. The increase in IL-6 also correlated with increased production of CRP, a marker of inflammation. However, IL-1Ra was increased in the elderly independently of CRP production. Although limited by the small control group, these data suggest that dysregulation of some inflammatory cytokines occurs with age, but the role of inflammation in aging remains unclear. C1 Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr Aging, Boston, MA 02111 USA. New England Med Ctr, Dept Med, Tupper Res Inst, Boston, MA 02111 USA. NIA, Geriatr Epidemiol Off, Epidemiol Demog & Biometry Program, Bethesda, MD 20892 USA. Framingham Heart Dis Epidemiol Study, Framingham, MA USA. RP Roubenoff, R (reprint author), Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM roubenoff@hnrc.tufts.edu FU NHLBI NIH HHS [N01-HC-38038]; NIA NIH HHS [Y01-AG-4-0245]; NIDDK NIH HHS [DK02120] NR 50 TC 237 Z9 241 U1 1 U2 12 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 USA SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD JAN PY 1998 VL 53 IS 1 BP M20 EP M26 PG 7 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA YU395 UT WOS:000071712800014 PM 9467429 ER PT J AU Rockwell, RC Abeles, RP AF Rockwell, RC Abeles, RP TI Guest editorial: Sharing and archiving data is fundamental to scientific progress SO JOURNALS OF GERONTOLOGY SERIES B-PSYCHOLOGICAL SCIENCES AND SOCIAL SCIENCES LA English DT Editorial Material C1 Univ Michigan, Ann Arbor, MI 48109 USA. NIA, Bethesda, MD 20892 USA. RP Rockwell, RC (reprint author), Univ Michigan, Ann Arbor, MI 48109 USA. NR 3 TC 6 Z9 6 U1 0 U2 2 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 USA SN 1079-5014 J9 J GERONTOL B-PSYCHOL JI J. Gerontol. Ser. B-Psychol. Sci. Soc. Sci. PD JAN PY 1998 VL 53 IS 1 BP S5 EP S8 PG 4 WC Geriatrics & Gerontology; Gerontology; Psychology; Psychology, Multidisciplinary SC Geriatrics & Gerontology; Psychology GA YV016 UT WOS:000071780300008 PM 9469174 ER PT S AU Weiss, SRB Li, XL Noguera, EC Heynen, T Li, H Rosen, JB Post, RM AF Weiss, SRB Li, XL Noguera, EC Heynen, T Li, H Rosen, JB Post, RM BE Corcoran, ME Moshe, SL TI Quenching - Persistent alterations in seizure and afterdischarge threshold following low-frequency stimulation SO KINDLING 5 SE ADVANCES IN BEHAVIORAL BIOLOGY LA English DT Proceedings Paper CT 5th International Conference on Kindling CY JUN 27-30, 1996 CL VICTORIA, CANADA SP Parke Davis, Warner Lambert Co, CIBA GENEVA, Hoechst Marion Roussel Inc, Univ Toronto, Bloorview Epilepsy Res Program, Abbott Labs, McNeil Pharm Corp, Wallace Labs C1 NIMH, Biol Psychiat Branch, Bethesda, MD 20892 USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0099-9962 BN 0-306-45805-5 J9 ADV BEHAV BIOL PY 1998 VL 48 BP 101 EP 119 PG 3 WC Behavioral Sciences; Neurosciences; Psychology, Experimental SC Behavioral Sciences; Neurosciences & Neurology; Psychology GA BK63A UT WOS:000072808800009 ER PT J AU Green, R AF Green, R TI Keynote panel: ISKO Fifth International Conference 1998, Lille, Paris SO KNOWLEDGE ORGANIZATION LA English DT Editorial Material AB ISKO's Fifth International Conference was held August 25-29, 1998 in Lille, France. The conference opened with a panel (Ia McIlwaine, A. Neelameghan, Michele Hudon, Christian Fluhr, Joan Mitchell, and Carol Bean; moderator, Rebecca Green) who addressed the general theme of the conference, Structures and Relations in knowledge Organization. Each panelist was given a brief period in which ro reflect on issues in one or more of three areas falling within the conference theme: (1) The role of hierarchical relationships in knowledge organization; (2) Relationships in multilingual, multicultural, and multidisciplinary contexts; and (3) Relationships in online retrieval. C1 Univ Maryland, Coll Lib & Informat Serv, College Pk, MD 20742 USA. UCL, Sch Lib Arch & Informat Studies, London WC1E 6BT, England. Univ Montreal, EBSI, Montreal, PQ H3C 3J7, Canada. Natl Lib Med, Lister Hill Natl Ctr Biomed Commun, UMLS, Bethesda, MD 20894 USA. Univ Toronto, Fac Informat Studies, Toronto, ON, Canada. RP Green, R (reprint author), Univ Maryland, Coll Lib & Informat Serv, College Pk, MD 20742 USA. NR 29 TC 0 Z9 0 U1 1 U2 1 PU ERGON-VERLAG PI WURZBURG PA GROMBUHLSTR 7, 97080 WURZBURG, GERMANY SN 0943-7444 J9 KNOWL ORGAN JI Knowl. Organ. PY 1998 VL 25 IS 4 BP 143 EP 161 PG 19 WC Information Science & Library Science SC Information Science & Library Science GA 201FY UT WOS:000080585400002 ER PT B AU Yaffe, SJ Shankaran, S AF Yaffe, SJ Shankaran, S BE Cosmi, EV TI Phenobarbital: does it prevent intracranial hemorrhage in preterm infants? SO LABOR AND DELIVERY LA English DT Proceedings Paper CT 2nd World Congress on Labor and Delivery CY MAY, 1997 CL ROME, ITALY SP Univ Rome La Sapienza, March Dimes, US, CNR, Italy, US NIH, US NICHHD, Italian Min Univ & Technol, Mother & Child Intl, MCI, IAMANEH, Italian Sect, World Soc Labor & Delivery, World & European Assoc Perinatal Med, Int Soc New Technol Gynecol Reproduct & Neonatol, Int Assoc Study Lung Surfactant Syst, Italian Soc Perinatal Med Neonatol Gynecol & Obstet C1 NICHHD, NIH, Bethesda, MD 20892 USA. RP Yaffe, SJ (reprint author), NICHHD, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PARTHENON PUBLISHING GROUP LTD PI LANCASTER PA CASTERTON HALL, CARNFORTH, LANCASTER LA6 2LA, ENGLAND BN 1-85070-973-4 PY 1998 BP 272 EP 277 PG 6 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA BL41F UT WOS:000075412400048 ER PT J AU Sanjuan, X Sobel, MF Yang, J Merino, MJ AF Sanjuan, X Sobel, MF Yang, J Merino, MJ TI Alveolar soft part sarcoma: correlation between genetic alterations, proliferative markers and clinico-pathologic findings. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1998 VL 78 IS 1 MA 65 BP 15A EP 15A PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA YU933 UT WOS:000071771200080 ER PT J AU Otis, CN Albuquerque, A Sanjuan, X Sobel, M Merino, MJ AF Otis, CN Albuquerque, A Sanjuan, X Sobel, M Merino, MJ TI Loss of heterozygosity in TP53, BRCA1, VHL, and estrogen receptor genes in breast carcinoma: Correlation to related protein products and morphologic features. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 Tufts Univ, Sch Med, Baystate Med Ctr, Springfield, MA 01199 USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1998 VL 78 IS 1 MA 122 BP 24A EP 24A PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA YU933 UT WOS:000071771200137 ER PT J AU Gerlach, C Kubbutat, M Schwab, U Key, G Flad, HD Gerdes, J AF Gerlach, C Kubbutat, M Schwab, U Key, G Flad, HD Gerdes, J TI Proliferation-associated Ki-67 protein is a target for autoantibodies in the human autoimmune disease systemic lupus erythematosus SO LABORATORY INVESTIGATION LA English DT Article ID CELL-PROLIFERATION; ANTIBODY KI-67; ANTIGEN; NITROCELLULOSE; ELEMENTS C1 Forschungszentrum Borstel, Dept Immunol & Cell Biol, D-23845 Borstel, Germany. Univ Lubeck, Dept Neurosurg, D-2400 Lubeck, Germany. NCI, Frederick, MD 21701 USA. Univ Kiel, Med & Poliklin, Kiel, Germany. Inst Chemo & Biosensor, Div Immunotechnol, Munster, Germany. RP Gerdes, J (reprint author), Forschungszentrum Borstel, Dept Immunol & Cell Biol, Parkallee 22, D-23845 Borstel, Germany. NR 10 TC 7 Z9 7 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1998 VL 78 IS 1 BP 129 EP 130 PG 2 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA YU933 UT WOS:000071771200014 PM 9461130 ER PT S AU Putney, JW Huang, Y Bird, GSJ AF Putney, JW Huang, Y Bird, GSJ BE Sullivan, DA Dartt, DA Meneray, MA TI Calcium signalling in lacrimal acinar cells SO LACRIMAL GLAND, TEAR FILM, AND DRY EYE SYNDROMES 2: BASIC SCIENCE AND CLINICAL RELEVANCE SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2nd International Conference on the Lacrimal Gland, Tear Film and Dry Eye Syndromes - Basic Science and Clinical Relevance CY NOV 16-19, 1996 CL SOUTHAMPTON, BERMUDA ID INOSITOL TRISPHOSPHATE; NONEXCITABLE CELLS; CYTOSOLIC CALCIUM; OSCILLATIONS; ENTRY C1 NIEHS, Calcium Regulat Sect, Lab Signal Transduct, NIH, Res Triangle Pk, NC 27709 USA. RP Putney, JW (reprint author), NIEHS, Calcium Regulat Sect, Lab Signal Transduct, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. NR 13 TC 5 Z9 5 U1 1 U2 2 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-45812-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 438 BP 123 EP 128 PG 6 WC Medicine, Research & Experimental; Ophthalmology SC Research & Experimental Medicine; Ophthalmology GA BL11R UT WOS:000074336100016 PM 9634874 ER PT S AU Smith, JA Chan, CC Egwuagu, CE Whitcup, SM AF Smith, JA Chan, CC Egwuagu, CE Whitcup, SM BE Sullivan, DA Dartt, DA Meneray, MA TI Immunohistochemical examination of lacrimal gland tissue from patients with ocular sarcoidosis SO LACRIMAL GLAND, TEAR FILM, AND DRY EYE SYNDROMES 2: BASIC SCIENCE AND CLINICAL RELEVANCE SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2nd International Conference on the Lacrimal Gland, Tear Film and Dry Eye Syndromes - Basic Science and Clinical Relevance CY NOV 16-19, 1996 CL SOUTHAMPTON, BERMUDA C1 NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. RP Smith, JA (reprint author), NEI, Immunol Lab, NIH, Bldg 10, Bethesda, MD 20892 USA. RI wei, chi/H-5730-2011 NR 7 TC 3 Z9 3 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-45812-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 438 BP 599 EP 602 PG 4 WC Medicine, Research & Experimental; Ophthalmology SC Research & Experimental Medicine; Ophthalmology GA BL11R UT WOS:000074336100084 PM 9634942 ER PT S AU Fox, PC Grisius, MM Bermudez, DK Sun, D AF Fox, PC Grisius, MM Bermudez, DK Sun, D BE Sullivan, DA Dartt, DA Meneray, MA TI Cytokine mRNA expression in labial salivary glands and cytokine secretion in parotid saliva in Sjogren's syndrome SO LACRIMAL GLAND, TEAR FILM, AND DRY EYE SYNDROMES 2: BASIC SCIENCE AND CLINICAL RELEVANCE SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2nd International Conference on the Lacrimal Gland, Tear Film and Dry Eye Syndromes - Basic Science and Clinical Relevance CY NOV 16-19, 1996 CL SOUTHAMPTON, BERMUDA ID BIOPSIES C1 NIDR, Clin Invest & Patient Care Branch, NIH, Bethesda, MD 20892 USA. RP Fox, PC (reprint author), NIDR, Clin Invest & Patient Care Branch, NIH, Bldg 10, Bethesda, MD 20892 USA. NR 10 TC 11 Z9 11 U1 0 U2 1 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-45812-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 438 BP 909 EP 915 PG 7 WC Medicine, Research & Experimental; Ophthalmology SC Research & Experimental Medicine; Ophthalmology GA BL11R UT WOS:000074336100129 PM 9634987 ER PT J AU Humphrey, RW Davis, DA Newcomb, FM Yarchoan, R AF Humphrey, RW Davis, DA Newcomb, FM Yarchoan, R TI Human herpesvirus 8 (HHV-8) in the pathogenesis of Kaposi's sarcoma and other diseases SO LEUKEMIA & LYMPHOMA LA English DT Review DE Kaposi's sarcoma; lymphoma; herpesvirus; KSHV; Castleman's disease ID EPSTEIN-BARR-VIRUS; MULTICENTRIC CASTLEMANS-DISEASE; PERIPHERAL-BLOOD MONOCYTES; AUTOCRINE GROWTH-FACTOR; DNA-SEQUENCES; ENDOTHELIAL-CELLS; HOMOSEXUAL MEN; (KS)-ASSOCIATED HERPESVIRUS; TRANSPLANT PATIENTS; HIV-INFECTION AB The discovery of Kaposi's Sarcoma-associated herpesvirus/human herpesvirus-8 (KSHV/HHV-8) and subsequent studies of this virus have provided a body of evidence that support the concept that this is an etiologic agent for Kaposi's sarcoma (KS). Several studies have indicated that this virus may also be a causal agent for primary effusion lymphoma (PEL) and Castleman's disease as well. First,generation serologic assays for HHV-8 have now been developed. The preponderance of data suggest that the incidence of HHV-8 infection is highest in populations at risk for KS: male homosexuals, immunosuppressed patients, and those who live in endemic regions. HHV-8 encodes for functional homologs of human proteins that may play a role in the development of disease. As we learn more about the steps by which this virus can lead to KS and/or other diseases, rational therapies and preventative strategies may be possible. C1 NCI, HIV & AIDS Malignancy Branch, Bethesda, MD 20892 USA. RP Yarchoan, R (reprint author), NCI, HIV & AIDS Malignancy Branch, Bethesda, MD 20892 USA. EM yarchoan@helix.nih.gov NR 73 TC 17 Z9 17 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1042-8194 J9 LEUKEMIA LYMPHOMA JI Leuk. Lymphoma PD JAN PY 1998 VL 28 IS 3-4 BP 255 EP 264 PG 10 WC Oncology; Hematology SC Oncology; Hematology GA YZ733 UT WOS:000072286000004 PM 9517497 ER PT J AU Wun, LM Merrill, RM Feuer, EJ AF Wun, LM Merrill, RM Feuer, EJ TI Estimating lifetime and age-conditional probabilities of developing cancer SO LIFETIME DATA ANALYSIS LA English DT Article DE life table; incidence; cancer prevalence; hysterectomy prevalence; corpus and uterus NOS cancers ID DEVELOPING BREAST-CANCER; RISK; PREVALENCE AB Lifetime and age-conditional risk estimates of developing cancer provide a useful summary to the public of the current cancer risk and how this risk compares with earlier periods and among select subgroups of society. These reported estimates, commonly quoted in the popular press, have the potential to promote early detection efforts, to increase cancer awareness, and to serve as an aid in study planning. However, they can also be easily misunderstood and frightening to the general public. The Surveillance, Epidemiology, and End Results (SEER) Program of the National Cancer Institute and the American Cancer Society have recently begun including in annual reports lifetime and age-conditional risk estimates of developing cancer. These risk estimates are based on incidence rates that reflect new cases of the cancer in a population free of the cancer. To compute these estimates involves a cancer prevalence adjustment that is computed cross-sectionally from current incidence and mortality data derived within a multiple decrement life table. This paper presents a detailed description of the methodology for deriving lifetime and age-conditional risk estimates of developing cancer. In addition, an extension is made which, using a triple decrement life table, adjusts for a surgical procedure that removes individuals from the risk of developing a given cancer. Two important results which provide insights into the basic methodology are included in the discussion. First, the lifetime risk estimate does not depend on the cancer prevalence adjustment, although this is not the case For age-conditional risk estimates. Second, the lifetime risk estimate is always smaller when it is corrected for a surgical procedure that takes people out of the risk pool to develop the cancer. The methodology is applied to corpus and uterus NOS cancers, with a correction made for hysterectomy prevalence. The interpretation and limitations of risk estimates are also discussed. C1 NCI, Canc Control Res Program, Appl Res Branch, Bethesda, MD 20892 USA. RP Wun, LM (reprint author), NCI, Canc Control Res Program, Appl Res Branch, Bethesda, MD 20892 USA. NR 24 TC 47 Z9 48 U1 0 U2 5 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 1380-7870 J9 LIFETIME DATA ANAL JI Lifetime Data Anal. PY 1998 VL 4 IS 2 BP 169 EP 186 DI 10.1023/A:1009685507602 PG 18 WC Mathematics, Interdisciplinary Applications; Statistics & Probability SC Mathematics GA 151AF UT WOS:000077698200005 PM 9658774 ER PT J AU Kuroiwa, T Lee, EG AF Kuroiwa, T Lee, EG TI Cellular interactions in the pathogenesis of lupus nephritis: The role of T cells and macrophages in the amplification of the inflammatory process in the kidney SO LUPUS LA English DT Review DE CD40 ligand; cell contact; intercellular adhesion molecule-1; monocyte chemoattractant protein-1; T(H)1 cytokine ID MONOCYTE CHEMOATTRACTANT PROTEIN-1; INCREASED NITRIC-OXIDE; TUBULAR EPITHELIAL-CELLS; HUMAN MESANGIAL CELLS; CLASS-II ANTIGENS; MRL-LPR/LPR MICE; EXPERIMENTAL GLOMERULONEPHRITIS; AUTOIMMUNE-DISEASE; MONOCLONAL-ANTIBODY; MURINE LUPUS AB A significant number of T cells and macrophages infiltrate the kidneys of patients with lupus nephritis. Chemotactic factors, especially monocyte chemoattractant factor-1 (MCP-1) and adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1), cooperatively facilitate recruitment of mononuclear cells into inflamed tissue. Increased expression of class II MHC molecules and CD40 on renal tubular epithelial cells coupled with upregulation of CD40 ligand (CD40L) and interleukin-2 receptor on infiltrating T cells suggest ongoing cellular immune responses. Recent studies employing knockout mice suggest that the T-H-1 cytokine interferon-gamma is an important cytokine in amplifying the local immune response of lupus nephritis. Infiltrating mononuclear cells exert their effects on resident renal cells through secretion of soluble factors and/or direct cell to cell contact. These interactions, among others, involve molecules such as CD40/CD40L and adhesion molecules. Studies to better define these molecules are in progress and may provide additional targets for therapeutic intervention. Thus, while autoantibody production and complement activation are the major players in initiating the inflammatory response in lupus nephritis, cellular immune mechanisms mediated through infiltrating mononuclear cells have an important role in its amplification and the progression of renal injury. C1 NIAMSD, Arthrit & Rheumatism Branch, NIH, Bethesda, MD 20892 USA. RP Kuroiwa, T (reprint author), NIAMSD, Arthrit & Rheumatism Branch, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. NR 73 TC 69 Z9 74 U1 0 U2 3 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 0961-2033 J9 LUPUS JI Lupus PY 1998 VL 7 IS 9 BP 597 EP 603 DI 10.1191/096120398678920712 PG 7 WC Rheumatology SC Rheumatology GA 151UF UT WOS:000077738500005 PM 9884096 ER PT J AU Grande, JP Balow, JE AF Grande, JP Balow, JE TI Renal biopsy in lupus nephritis SO LUPUS LA English DT Review DE lupus nephritis; glomerulonephritis; renal biopsy; renal pathology ID KIDNEY BIOPSY; ERYTHEMATOSUS; GLOMERULONEPHRITIS; CLASSIFICATION; INFORMATION; NEPHROPATHY; PREDICTORS; PROGNOSIS AB Renal biopsy can be extremely valuable in the management of patients with lupus nephritis. It is remarkably common to find pathological evidence of substantial nephron loss in patients with low-grade laboratory abnormalities. This is due to compensatory hypertrophy and hemodynamic adjustments within the less-diseased nephron mass. It has been shown that the decision to institute immunosuppressive therapy is highly informed by the results of renal biopsy and offers the prospect of achieving more favorable renal outcomes. Kidney biopsies should be evaluated by dedicated renal pathology services experienced in diagnostic light, immunofluorescence and electron microscopy. Biopsies should be classified according to the World Health Organization (WHO) system and specific lesions semiquantitatively scored against a checklist of features comprising activity (reversible) and chronicity (irreversible damage) indices. The renal biopsy findings should be reviewed jointly by pathologists and the clinicians caring for patients with lupus nephritis. C1 Mayo Clin & Mayo Fdn, Dept Internal Med, Div Nephrol, Rochester, MN 55905 USA. Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, Div Anat Pathol, Rochester, MN 55905 USA. NIDDKD, Kidney Dis Sect, NIH, Bethesda, MD 20892 USA. RP Grande, JP (reprint author), Mayo Clin & Mayo Fdn, Dept Internal Med, Div Nephrol, 200 1st St SW,Hilton 11, Rochester, MN 55905 USA. NR 39 TC 45 Z9 50 U1 0 U2 1 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 0961-2033 J9 LUPUS JI Lupus PY 1998 VL 7 IS 9 BP 611 EP 617 DI 10.1191/096120398678920730 PG 7 WC Rheumatology SC Rheumatology GA 151UF UT WOS:000077738500007 PM 9884098 ER PT J AU Austin, HA AF Austin, HA TI Clinical evaluation and monitoring of lupus kidney disease SO LUPUS LA English DT Review DE glomerular filtration rate; kidney function tests; lupus nephritis; proteinuria ID LONG-TERM; GLOMERULAR-FILTRATION; CREATININE CLEARANCE; ORAL CIMETIDINE; RENAL-FUNCTION; NEPHRITIS; ERYTHEMATOSUS; PREDICTOR; RELAPSES; BIOPSY AB Lupus renal involvement encompasses a broad range of clinical and histologic presentations and poses numerous therapeutic challenges. Accurate clinical assessment and monitoring requires a comprehensive approach, with attention to the urinalysis, the urinary protein excretion rate, the test of kidney function, and alterations in serologic parameters. C1 NIH, Kidney Dis Sect, Bethesda, MD 20892 USA. RP Austin, HA (reprint author), NIH, Kidney Dis Sect, Bldg 10,Room 3N112,9000 Rockville Pike, Bethesda, MD 20892 USA. NR 28 TC 27 Z9 30 U1 0 U2 1 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 0961-2033 J9 LUPUS JI Lupus PY 1998 VL 7 IS 9 BP 618 EP 621 DI 10.1191/096120398678920749 PG 4 WC Rheumatology SC Rheumatology GA 151UF UT WOS:000077738500008 PM 9884099 ER PT J AU Boumpas, DT Balow, JE AF Boumpas, DT Balow, JE TI Outcome criteria for lupus nephritis trials: A critical overview SO LUPUS LA English DT Review DE hematuria; proteinuria; relapse; remission; serology ID PULSE CYCLOPHOSPHAMIDE; IGA NEPHROPATHY; FISH-OIL; GLOMERULONEPHRITIS; ERYTHEMATOSUS; PREDNISONE; RELAPSE; METHYLPREDNISOLONE; THERAPY AB Lupus nephritis is an important cause of morbidity and mortality in patients with systemic lupus erythematosus. Traditional outcome criteria such as doubling of serum creatinine, end-stage renal disease and death have been used in controlled therapeutic trials, but are limited by their low incidence and the extended period of time required to reach them. More recently, discussions have focused on composite outcome measures, such as remission and relapse, as well as measures of health-related quality of life, general lupus activity and cumulative damage indexes. We review the strengths and weaknesses of several outcome criteria, and we propose criteria for bath small pilot studies and large definitive trials. C1 NIAMSD, Clin Invest Sect, Arthrit & Rheumatism Branch, NIH, Bethesda, MD 20892 USA. NIDDKD, Kidney Dis Sect, NIH, Bethesda, MD 20892 USA. RP Boumpas, DT (reprint author), NIAMSD, Clin Invest Sect, Arthrit & Rheumatism Branch, NIH, Bldg 10,Room 9S209,10 Ctr Dr MSC 1828, Bethesda, MD 20892 USA. NR 37 TC 64 Z9 67 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 0961-2033 J9 LUPUS JI Lupus PY 1998 VL 7 IS 9 BP 622 EP 629 DI 10.1191/096120398678920758 PG 8 WC Rheumatology SC Rheumatology GA 151UF UT WOS:000077738500009 PM 9884100 ER PT J AU Illei, GG Klippel, JH AF Illei, GG Klippel, JH TI Novel approaches in the treatment of lupus nephritis SO LUPUS LA English DT Review DE biological products; clinical trial; human; systemic lupus erythematosus; therapy ID SUBSEQUENT PULSE CYCLOPHOSPHAMIDE; A(2) SYNTHETASE INHIBITOR; ANTI-DNA ANTIBODY; MURINE LUPUS; RENAL-FUNCTION; MYCOPHENOLATE MOFETIL; MONOCLONAL-ANTIBODY; DEOXYRIBONUCLEASE-I; AUTOIMMUNE-DISEASE; CD40 LIGAND AB Lupus nephritis can be managed successfully in the majority of cases; most therapies, however, are associated with significant side-effects. Several new agents aiming at specific stages in the pathogenesis of lupus are in different phases of clinical trials. The central role of lymphocytes makes them targets of various therapeutic approaches. Lymphocyte depletion can be achieved by high-dose chemotherapy with or without bone marrow transplantation. Nucleoside analogs selectively deplete mononuclear cells; antibodies against T or B cell surface antigens target specific subsets of lymphocytes. Synchronized plasmapheresis has been used in an attempt to delete pathogenic lymphocyte clones activated by plasmapheresis. Treating patients with DNase or neutralizing pathogenic antibodies by administering specific binding peptides or inducing specific anti-idiotype antibodies may prevent immune complex formation and/or deposition. Blocking the complement cascade or some of the inflammatory mediators like thromboxane A(2) may be efficacious even if immune complex deposition could not be prevented. Inducing antigen-specific tolerance or interfering with important interactions between T-lymphocytes and other cells by blocking CD40 ligand or decreasing the level of interleukin-10 are some of the other approaches currently under clinical investigation. C1 NIAMSD, Arthrit & Rheumatism Branch, NIH, Bethesda, MD 20892 USA. RP Illei, GG (reprint author), NIAMSD, Arthrit & Rheumatism Branch, NIH, 10 9S209 10 Ctr Dr MSC 1828, Bethesda, MD 20892 USA. NR 59 TC 16 Z9 17 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 0961-2033 J9 LUPUS JI Lupus PY 1998 VL 7 IS 9 BP 644 EP 648 DI 10.1191/096120398678920794 PG 5 WC Rheumatology SC Rheumatology GA 151UF UT WOS:000077738500013 PM 9884104 ER PT J AU Crane, M Pucino, F Sebring, N Irby, D Perry, M Mattiko, M Yarboro, C AF Crane, M Pucino, F Sebring, N Irby, D Perry, M Mattiko, M Yarboro, C TI The interdisciplinary team's approach to lupus nephritis SO LUPUS LA English DT Review DE chronic disease; interdisciplinary team; lupus nephritis ID DISEASE-INDUCED VARIATIONS; PLASMA-PROTEIN LEVELS; DRUG-DOSAGE REGIMENS; ERYTHEMATOSUS; ANTIBODIES AB The interdisciplinary team approach in assessment and treatment of patients with chronic disease in general and lupus nephritis in particular provides a global format for identifying the multiple problem areas that retard or prevent optimal patient functioning. These areas include the physical, emotional, economic, psychosocial, and functional. Benefits to the individual patient include a thorough multifaceted assessment by professionals who have the benefit of peer collaboration and validation. This increases the likelihood that the whole patient is considered, not just the problem of nephritis. For example, how does the patient and her or his family cope with the impact of such a disease and how, in turn, do the coping abilities of the patient and family affect the disease. The interdisciplinary team also assesses how the treatment strategies for each problem area influence each other. Finally, the interdisciplinary team serves as a positive role model for effective collaboration among health professionals and for students in their respective disciplines. C1 NIAMSD, Arthrit & Rheumatism Branch, NIH, Bethesda, MD 20892 USA. NIH, Warren Grant Magnuson Clin Ctr, Dept Clin Immunol & Pharm, Bethesda, MD 20892 USA. NIH, Warren Grant Magnuson Clin Ctr, Dept Nutr, Bethesda, MD 20892 USA. NIH, Warren Grant Magnuson Clin Ctr, Dept Social Work, Bethesda, MD 20892 USA. NIH, Warren Grant Magnuson Clin Ctr, Dept Rehabil Med, Bethesda, MD 20892 USA. RP Crane, M (reprint author), NIAMSD, Arthrit & Rheumatism Branch, NIH, Bldg 10 Room 9S205,10 Ctr Dr MSC 1828, Bethesda, MD 20892 USA. NR 19 TC 7 Z9 7 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 0961-2033 J9 LUPUS JI Lupus PY 1998 VL 7 IS 9 BP 660 EP 665 DI 10.1191/096120398678920820 PG 6 WC Rheumatology SC Rheumatology GA 151UF UT WOS:000077738500016 PM 9884107 ER PT B AU Paylor, R Bowers, BJ Wehner, JM AF Paylor, R Bowers, BJ Wehner, JM BE Neugebauer, A TI The involvement of protein kinase C in hippocampal-dependent learning SO MACROMOLECULAR INTERPLAY IN BRAIN ASSOCIATIVE MECHANISMS SE SERIES ON BIOPHYSICS AND BIOCYBERNETICS LA English DT Proceedings Paper CT International School of Biocybernetics - Macromolecular Interplay in Brain Associative Mechanisms CY OCT 16-21, 1995 CL NAPLES, ITALY AB Recent findings indicate that protein kinase C (PKC) contributes to learning and memory. We have examined the role of PKC in spatial learning using a variety of approaches. In the first study, rats were treated with the PKC activator, PDBu, and tested in the Morris water task. PDBu-treated subjects located the platform faster than controls and remembered its location better when tested 24 hours later. Next, the affects of rearing in an enriched environment on spatial learning and hippocampal PKC activity were studied. Rats were reared either in an enriched environment or in standard laboratory cages starting at 15 days of age. Results showed that the performance of rats exposed to an enriched environment for 12 days, but not 6 days, was better than control rats. Additionally, enriched rearing increased hippocampal PKC activity. The final study examined the development of spatial learning and hippocampal PKC in C57 and DBA mice. Neither strain of mice could solve the spatial-learning task at 17 days of age, but C57 mice were able to learn the task at 24 days of age. This behavioral dissociation was paralleled by changes in levels of hippocampal PKC. Together, these findings clearly indicate that PKC is involved in spatial learning. C1 NIMH, Sect Behav Neuropharmacol, Expt Therapeut Branch, NIH, Bethesda, MD 20892 USA. RP Paylor, R (reprint author), NIMH, Sect Behav Neuropharmacol, Expt Therapeut Branch, NIH, Bldg 10,Rm 4N212, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WORLD SCIENTIFIC PUBL CO PTE LTD PI SINGAPORE PA PO BOX 128 FARRER RD, SINGAPORE 9128, SINGAPORE BN 981-02-3212-8 J9 SER BIOPHYS BIOCYBER PY 1998 VL 4 BP 240 EP 249 PG 10 WC Neurosciences SC Neurosciences & Neurology GA BM19C UT WOS:000077948300025 ER PT J AU Yang, YH Glover, GH van Gelderen, P Patel, AC Mattay, VS Frank, JA Duyn, JH AF Yang, YH Glover, GH van Gelderen, P Patel, AC Mattay, VS Frank, JA Duyn, JH TI A comparison of fast MR scan techniques for cerebral activation studies at 1.5 Tesla SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article DE MRI; spiral scan; EPI; fMRI ID HUMAN BRAIN; SENSORY STIMULATION; PHOTIC-STIMULATION; SIGNAL CHANGES; OXYGENATION; CORTEX; FMRI AB To evaluate the sensitivity of fast, gradient-echo MR scan techniques in their ability to detect blood oxygenation level dependent (BOLD) signal changes in task activation studies, three dedicated fast scan techniques, each with whole-brain coverage, were compared during a 3-min finger tapping paradigm on nine normal volunteers on a clinical 1.5 T scanner, Multislice (2D) single-shot spiral, 3D spiral, and multislice (2D) single-shot EPI scan techniques were done with similar temporal and spatial resolutions on each of the volunteers in random order, After image registration and statistical analysis, the sensitivity to detect activation was evaluated for the techniques by calculating t scores and number of activated voxels in predetermined regions of interest, including the contralateral primary sensorimotor cortex, the premotor region, the parietal region, the supplementary motor area, and the ipsilateral cerebellum. Baseline images acquired with the three techniques were qualitatively comparable and had a similar effective spatial resolution of around 5 x 5 x 5 mm(3), as determined from autocorrelation analysis, The anatomical coverage was somewhat reduced (4 less slices per volume) with EPI at the identical temporal resolution of 1.76 s for all techniques. The use of multislice 2D spiral scan for motor cortex fMRI experiments provided for a superior overall temporal stability, and an increased sensitivity compared with multislice 2D EPI, and 3D spiral scan, The difference in sensitivity between multislice 2D spiral and EPI scans was small, in particular in the case of a ramp-sampled version of EPI. The difference in performance is attributed mainly to the difference in scan-to-scan stability. C1 NIMH, Lab Diagnost Radiol Res, OIR, NIH, Bethesda, MD 20892 USA. NIMH, In Vivo NMR Res Ctr, BEIP,NCRR, NIH, Bethesda, MD 20892 USA. NIMH, Clin Brain Disorders Branch, NIH, Bethesda, MD 20892 USA. Stanford Univ, Sch Med, Lucas MRI Ctr, Dept Diagnost Radiol, Stanford, CA 94305 USA. RP Duyn, JH (reprint author), NIMH, Lab Diagnost Radiol Res, OIR, NIH, Bldg 10,Room BIN-256, Bethesda, MD 20892 USA. RI Duyn, Jozef/F-2483-2010; Patel, Anand/B-1441-2009 OI Patel, Anand/0000-0002-6570-8582 NR 29 TC 47 Z9 47 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0740-3194 J9 MAGNET RESON MED JI Magn.Reson.Med. PD JAN PY 1998 VL 39 IS 1 BP 61 EP 67 DI 10.1002/mrm.1910390111 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA YN369 UT WOS:000071161400010 PM 9438438 ER PT J AU Fernandez, MP Copeland, NG Gilbert, DJ Jenkins, NA Morgan, RO AF Fernandez, MP Copeland, NG Gilbert, DJ Jenkins, NA Morgan, RO TI The genetic origin of mouse annexin VIII SO MAMMALIAN GENOME LA English DT Article ID ACUTE PROMYELOCYTIC LEUKEMIA; EXPRESSION; SEQUENCE; RATES; LOCALIZATION; ORGANIZATION; SUBSTITUTION; ALIGNMENT; PHYLOGENY; PROTEIN AB Mouse annexin VIII cDNA was characterized by DNA sequencing of expressed sequence tag clones, molecular systematic analysis, and genetic linkage mapping to investigate its evolutionary origin. Its subfamily identity, divergence pattern, and nucleotide substitution rate were established by comparison with other annexin cDNA and deduced protein sequences. The known phylogenetic association of annexin VIII in an evolutionary clade with annexins XI, IV, V, and VIa identified these close homologs as potential progenitors or duplication products. Cladistic analysis confirmed the base position of annexin XI and its relationship to annexin TV as a direct duplication product. Although annexin Vm also derived from annexin XI, the evolutionary branching order, gene separation times, and mapping results indicated that it was probably a subsequent duplication product of annexin IV about 300 million years ago. Dates were calibrated against the assumed separation time of 75 Mya for rodents from other mammals, divergence rates were based on comparisons of all available annexin species, and relative rate tests implied individually stable gene clocks for most annexins. Linkage mapping of mouse Anus to the centromeric region of Chromosome (Chr) 14 placed it in a more distal homology group from previously mapped Anx7 and Anx11. Despite their synteny, the combined proximity and segregation of these three annexins diminished the likelihood that they were mutual gene duplication products. C1 Univ Oviedo, Fac Med, Dept Biochem & Mol Biol, E-33006 Oviedo, Spain. NCI, Frederick Canc Res & Dev Ctr, Mammalian Genet Lab, ABL Basic Res Program, Frederick, MD 21702 USA. RP Univ Oviedo, Fac Med, Dept Biochem & Mol Biol, E-33006 Oviedo, Spain. OI Fernandez-Fernandez, Maria Pilar/0000-0001-6552-409X NR 32 TC 4 Z9 5 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0938-8990 EI 1432-1777 J9 MAMM GENOME JI Mamm. Genome PD JAN PY 1998 VL 9 IS 1 BP 8 EP 14 DI 10.1007/s003359900671 PG 7 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA YN895 UT WOS:000071219500003 PM 9434938 ER PT J AU Chaudhary, R Raudsepp, T Guan, XY Zhang, HG Chowdhary, BP AF Chaudhary, R Raudsepp, T Guan, XY Zhang, HG Chowdhary, BP TI Zoo-FISH with microdissected arm specific paints for HSA2, 5, 6, 16, and 19 refines known homology with pig and horse chromosomes SO MAMMALIAN GENOME LA English DT Article ID RAPID GENERATION; HUMAN GENOMES; SEGMENTS; PROBES; REVEALS; SYNTENY; PORCINE; CATTLE; MAP; DNA AB Microdissected arm specific paints (ASPs) for human (HSA) chromosomes (Chrs) 2, 5, 6, 16, and 19 were used as probes on pig (SSC) and horse (EGA) metaphase chromosomes. Regions homologous to individual human arms were delineated in the two species studied. Of the ten ASPs used, HSA6 and 16 ASPs showed complete synteny conservation of individual arms as single blocks/ arms both in pig and horse. A similar trend was, in general, also observed for HSA19 ASPs. However, contrary to these observations, synteny conservation of individual arms of HSA2 and HSA5 was not observed in pig and horse. The arm specific painting data, coupled with the available gene mapping data, showed that, although HSA2 corresponded to two arms/chromosomes each in pig and horse, the breakpoint of this synteny in humans was not located at the centromere, but at HSA2q13 band. Similarly, arm specific paints for HSA5 showed that of the two blocks/chromosomes painted in pig and horse, one corresponded to HSA5q13-pter, the other to HSA5q13-qter. The findings suggest that 5q13 band may also be an evolutionary break point, similar to the one detected on HSA2q13. The microdissected human arm specific painting probes used in the present work provide more accurate and refined comparative information on pig and horse chromosomes than that available through the use of human whole chromosome specific paints. C1 Swedish Univ Agr Sci, Dept Anim Breeding & Genet, S-75007 Uppsala, Sweden. NIH, Natl Ctr Human Genome Res, Bethesda, MD 20892 USA. RP Chowdhary, BP (reprint author), Swedish Univ Agr Sci, Dept Anim Breeding & Genet, Box 7023, S-75007 Uppsala, Sweden. RI Guan, Xin-Yuan/A-3639-2009 OI Guan, Xin-Yuan/0000-0002-4485-6017 NR 31 TC 22 Z9 22 U1 1 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD JAN PY 1998 VL 9 IS 1 BP 44 EP 49 DI 10.1007/s003359900677 PG 6 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA YN895 UT WOS:000071219500009 PM 9434944 ER PT J AU Torrey, T Kim, W Morse, HC Kozak, CA AF Torrey, T Kim, W Morse, HC Kozak, CA TI Cloning, expression and genetic mapping of the mouse SH3 domain protein, SH3D2B SO MAMMALIAN GENOME LA English DT Article C1 NIAID, Mol Microbiol Lab, Bethesda, MD 20892 USA. NIAID, Immunopathol Lab, NIH, Bethesda, MD 20892 USA. RP Kozak, CA (reprint author), NIAID, Mol Microbiol Lab, Bldg 4,Room 329, Bethesda, MD 20892 USA. OI Morse, Herbert/0000-0002-9331-3705 NR 9 TC 6 Z9 6 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD JAN PY 1998 VL 9 IS 1 BP 74 EP 75 DI 10.1007/s003359900683 PG 2 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA YN895 UT WOS:000071219500015 PM 9434950 ER PT J AU Fujii, TA Cvecklova, K Gilbert, DJ Copeland, NG Jenkins, NA Westphal, H AF Fujii, TA Cvecklova, K Gilbert, DJ Copeland, NG Jenkins, NA Westphal, H TI Genomic structure and chromosomal localization of the murine LIM class homeobox gene Lhx1 SO MAMMALIAN GENOME LA English DT Article ID EXPRESSION; PROTEINS; DOMAIN; XLIM-1 C1 NICHHD, Lab Mammalian Genes & Dev, NIH, Bethesda, MD 20892 USA. NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Mammalian Genet Lab, Frederick, MD 21702 USA. RP Westphal, H (reprint author), NICHHD, Lab Mammalian Genes & Dev, NIH, Bldg 6B,Room 413, Bethesda, MD 20892 USA. NR 18 TC 6 Z9 6 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD JAN PY 1998 VL 9 IS 1 BP 81 EP 83 DI 10.1007/s003359900686 PG 3 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA YN895 UT WOS:000071219500018 PM 9434953 ER PT J AU Rajan, L Kozak, CA Dudley, JP AF Rajan, L Kozak, CA Dudley, JP TI Chromosomal localization of acquired MMTV proviral integration sites in T-cell lymphomas SO MAMMALIAN GENOME LA English DT Article ID MAMMARY-TUMOR PROVIRUSES; VIRUS; GENE; MURINE C1 Univ Texas, Dept Microbiol, Austin, TX 78712 USA. NIAID, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA. RP Dudley, JP (reprint author), Univ Texas, Dept Microbiol, Austin, TX 78712 USA. FU NCI NIH HHS [R01 CA34780] NR 16 TC 2 Z9 2 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD JAN PY 1998 VL 9 IS 1 BP 84 EP 85 DI 10.1007/s003359900687 PG 2 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA YN895 UT WOS:000071219500019 PM 9434954 ER PT J AU Gopalan, G Gilbert, DJ Copeland, NG Jenkins, NA Donovan, PJ AF Gopalan, G Gilbert, DJ Copeland, NG Jenkins, NA Donovan, PJ TI Chromosome localization of two new mammalian kinases related to yeast and fly chromosome segregation-regulators SO MAMMALIAN GENOME LA English DT Article ID MOUSE; MAP C1 NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Mammalian Genet Lab, Frederick, MD 21702 USA. RP Donovan, PJ (reprint author), NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Mammalian Genet Lab, POB B, Frederick, MD 21702 USA. NR 11 TC 5 Z9 5 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD JAN PY 1998 VL 9 IS 1 BP 86 EP 87 DI 10.1007/s003359900688 PG 2 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA YN895 UT WOS:000071219500020 PM 9434955 ER PT J AU Burmeister, M Bryda, EC Bureau, JF Noben-Trauth, K AF Burmeister, M Bryda, EC Bureau, JF Noben-Trauth, K TI Mouse Chromosome 10 SO MAMMALIAN GENOME LA English DT Article; Proceedings Paper CT 11th International Mouse Genome Conference CY OCT 12-16, 1997 CL ST PETERSBURG, FLORIDA SP Natl Genome Res Inst, U S Dept Energy Off Health & Environm Res, Natl Inst Child Health & Human Dev, Natl Cancer Inst & Natl Ctr Res Resources Natl Inst Health, Off Res Technol & Graduate Educ Univ FL, Int Mammalian Genome Soc, Jackson Lab, Mouse Genome, Blackwell Sci Ltd, Book Publ, CuraGen Corp, Darwin Molec Corp, Millennium Pharmaceut, Inc, Oncor, Inc, PharMingen, Res Genet, Inc C1 Univ Michigan, Mental Hlth Res Inst, Ann Arbor, MI 48109 USA. Marshall Univ, Sch Med, Dept Microbiol Mol Genet & Immunol, Huntington, WV 25704 USA. Inst Pasteur, ESR 572, Unite Virus Lents, F-75724 Paris, France. Natl Inst Deafness & Other Commun Disorders, NIH, Rockville, MD 20850 USA. RP Burmeister, M (reprint author), Univ Michigan, Mental Hlth Res Inst, 205 Zina Pitcher Pl, Ann Arbor, MI 48109 USA. RI Burmeister, Margit/A-3157-2013 OI Burmeister, Margit/0000-0002-1914-2434 FU NIDCD NIH HHS [DC02982]; NINDS NIH HHS [NS32130] NR 0 TC 5 Z9 5 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PY 1998 VL 8 SI SI BP S200 EP S214 DI 10.1007/s003359900655 PG 15 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA 102GX UT WOS:000074917000010 PM 9662627 ER PT J AU Huppi, K Siwarski, D Letts, V AF Huppi, K Siwarski, D Letts, V TI Mouse Chromosome 15 SO MAMMALIAN GENOME LA English DT Article; Proceedings Paper CT 11th International Mouse Genome Conference CY OCT 12-16, 1997 CL ST PETERSBURG, FLORIDA SP Natl Genome Res Inst, U S Dept Energy Off Health & Environm Res, Natl Inst Child Health & Human Dev, Natl Cancer Inst & Natl Ctr Res Resources Natl Inst Health, Off Res Technol & Graduate Educ Univ FL, Int Mammalian Genome Soc, Jackson Lab, Mouse Genome, Blackwell Sci Ltd, Book Publ, CuraGen Corp, Darwin Molec Corp, Millennium Pharmaceut, Inc, Oncor, Inc, PharMingen, Res Genet, Inc C1 NCI, Genet Lab, NIH, Bethesda, MD 20892 USA. Jackson Lab, Bar Harbor, ME 04609 USA. RP Huppi, K (reprint author), NCI, Genet Lab, NIH, Bldg 37,Rm 2B-21, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PY 1998 VL 8 SI SI BP S292 EP S306 DI 10.1007/s003359900660 PG 15 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA 102GX UT WOS:000074917000015 PM 9662632 ER PT J AU Kozak, CA Stephenson, DA AF Kozak, CA Stephenson, DA TI Mouse Chromosome 5 SO MAMMALIAN GENOME LA English DT Article; Proceedings Paper CT 11th International Mouse Genome Conference CY OCT 12-16, 1997 CL ST PETERSBURG, FL SP Natl Genome Res Inst, U S Dept Energy Off Health & Environm Res, Natl Inst Child Health & Human Dev, Natl Cancer Inst & Natl Ctr Res Resources Natl Inst Health, Off Res Technol & Graduate Educ Univ FL, Int Mammalian Genome Soc, Jackson Lab, Mouse Genome, Blackwell Sci Ltd, Book Publ, CuraGen Corp, Darwin Molec Corp, Millennium Pharmaceut, Inc, Oncor, Inc, PharMingen, Res Genet, Inc C1 NIAID, Mol Microbiol Lab, Bethesda, MD 20892 USA. UCL, Dept Genet & Biometry, London NX1 2HE, England. RP Kozak, CA (reprint author), NIAID, Mol Microbiol Lab, Bethesda, MD 20892 USA. NR 0 TC 10 Z9 10 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PY 1998 VL 8 SI SI BP S91 EP S113 DI 10.1007/s003359900650 PG 23 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA 102GX UT WOS:000074917000005 PM 9662622 ER PT J AU Mock, BA Hirano, MC AF Mock, BA Hirano, MC TI Mouse Chromosome 4 SO MAMMALIAN GENOME LA English DT Article; Proceedings Paper CT 11th International Mouse Genome Conference CY OCT 12-16, 1997 CL ST PETERSBURG, FLORIDA SP Natl Genome Res Inst, U S Dept Energy Off Health & Environm Res, Natl Inst Child Health & Human Dev, Natl Cancer Inst & Natl Ctr Res Resources Natl Inst Health, Off Res Technol & Graduate Educ Univ FL, Int Mammalian Genome Soc, Jackson Lab, Mouse Genome, Blackwell Sci Ltd, Book Publ, CuraGen Corp, Darwin Molec Corp, Millennium Pharmaceut, Inc, Oncor, Inc, PharMingen, Res Genet, Inc C1 NCI, Genet Lab, DBS, NIH, Bethesda, MD 20892 USA. RP Mock, BA (reprint author), NCI, Genet Lab, DBS, NIH, Bldg 37,Room 2B-08,37 Convent Dr,MSC 4255, Bethesda, MD 20892 USA. NR 0 TC 6 Z9 6 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PY 1998 VL 8 SI SI BP S68 EP S90 DI 10.1007/s003359900649 PG 23 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA 102GX UT WOS:000074917000004 PM 9662621 ER PT J AU Stephenson, DA Lueders, KK AF Stephenson, DA Lueders, KK TI Mouse Chromosome 13 SO MAMMALIAN GENOME LA English DT Article; Proceedings Paper CT 11th International Mouse Genome Conference CY OCT 12-16, 1997 CL ST PETERSBURG, FL SP Natl Genome Res Inst, U S Dept Energy Off Health & Environm Res, Natl Inst Child Health & Human Dev, Natl Cancer Inst & Natl Ctr Res Resources Natl Inst Health, Off Res Technol & Graduate Educ Univ FL, Int Mammalian Genome Soc, Jackson Lab, Mouse Genome, Blackwell Sci Ltd, Book Publ, CuraGen Corp, Darwin Molec Corp, Millennium Pharmaceut, Inc, Oncor, Inc, PharMingen, Res Genet, Inc C1 UCL, Dept Biol, Galton Lab, London NW1 2HE, England. NCI, Biochem Lab, NIH, Bethesda, MD 20892 USA. RP Stephenson, DA (reprint author), UCL, Dept Biol, Galton Lab, Wolfson House,4 Stephenson Way, London NW1 2HE, England. NR 0 TC 3 Z9 3 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PY 1998 VL 8 SI SI BP S258 EP S274 DI 10.1007/s003359900658 PG 17 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA 102GX UT WOS:000074917000013 PM 9662630 ER PT S AU Callahan, R AF Callahan, R BE Rudland, PS Fernig, DG Leinster, S Lunt, GG TI Somatic mutations that contribute to breast cancer SO MAMMARY DEVELOPMENT IN CANCER SE Biochemical Society Symposia LA English DT Article; Proceedings Paper CT Annual Symposium on Mammary Development and Cancer, as Part of the 658th Meeting of the Biochemical-Society CY APR 16-18, 1996 CL UNIV LIVERPOOL, LIVERPOOL, ENGLAND SP Univ Liverpool, Dept Biochem, Biochem Soc, Canc & Polio Res Fund, Zeneca Pharm HO UNIV LIVERPOOL ID MAMMARY-TUMOR VIRUS; PROVIRAL INSERTION; EPITHELIAL-CELLS; DNA-SEQUENCES; NOTCH-GENE; MOUSE; HETEROZYGOSITY; EXPRESSION; REGION; CARCINOMAS AB Cytogenetic and molecular analyses of primary sporadic human breast carcinomas have documented at least 12 different chromosome arms affected by loss of heterozygosity (LOH). This has been taken as evidence for the presence of putative tumour suppresser genes in the remaining allele within the affected regions. We have previously identified three regions on chromosome 17q that are affected by LOH in primary human breast tumours. A physical map of one of these regions (17q21) has been prepared. The putative target gene appears to be located between the D17S846 and D17S746 loci. We are currently determining whether either of two genes located in this region is the target for LOH. The mouse mammary tumour model system provides an approach for identifying genes which, when activated or inactivated by mouse mammary tumour virus (MMTV) integration, contribute to specific stages of mammary tumorigenesis. Using this approach we have identified two genes, designated NOTCH4/INT3 and INT6 respectively. Interruption of NOTCH4/INT3 by MMTV represents a gain-of-function mutation that has profound consequences for mammary gland development and tumorigenesis. INT6 was found to be interrupted by an integrated MMTV genome in a mammary hyperplastic outgrowth line and two independent mammary tumours. In each case the transcriptional orientation of the viral genome was opposite to that of INT6. The rearranged allele was expressed as a truncated chimaeric RNA species composed of INT6 coding sequences, intron sequences and MMTV sequences. Since the non-rearranged allele contained no mutations, we conclude that MMTV integration into INT6 causes a dominant-negative mutation or biologically activates its function. The nucleotide sequence of INT6 is unrelated to any of the known genes in the GenBank database, but is evolutionarily highly conserved. C1 NCI, Oncogenet Sect, Tumor Immunol & Biol Lab, Bethesda, MD 20892 USA. RP Callahan, R (reprint author), NCI, Oncogenet Sect, Tumor Immunol & Biol Lab, Bldg 10,Room 5B50, Bethesda, MD 20892 USA. NR 62 TC 2 Z9 2 U1 0 U2 0 PU PORTLAND PRESS LTD PI LONDON PA 59 PORTLAND PL, LONDON W1N 3AJ, ENGLAND SN 0067-8694 BN 1-85578-087-9 J9 BIOCHEM SOC SYMP JI Biochem. Soc. Symp. PY 1998 IS 63 BP 211 EP 221 PG 11 WC Biochemistry & Molecular Biology; Oncology; Developmental Biology SC Biochemistry & Molecular Biology; Oncology; Developmental Biology GA BK13Y UT WOS:000071307400019 ER PT S AU Freije, JMP MacDonald, NJ Steeg, PS AF Freije, JMP MacDonald, NJ Steeg, PS BE Rudland, PS Fernig, DG Leinster, S Lunt, GG TI Nm23 and tumour metastasis: basic and translational advances SO MAMMARY DEVELOPMENT IN CANCER SE BIOCHEMICAL SOCIETY SYMPOSIUM LA English DT Article; Proceedings Paper CT Annual Symposium on Mammary Development and Cancer, as Part of the 658th Meeting of the Biochemical-Society CY APR 16-18, 1996 CL UNIV LIVERPOOL, LIVERPOOL, ENGLAND SP Univ Liverpool, Dept Biochem, Biochem Soc, Canc & Polio Res Fund, Zeneca Pharm HO UNIV LIVERPOOL ID NUCLEOSIDE-DIPHOSPHATE KINASE; HUMAN BREAST-CANCER; SQUAMOUS-CELL CARCINOMA; LYMPH-NODE METASTASIS; HEPATOCELLULAR-CARCINOMA; MYXOCOCCUS-XANTHUS; PROTEIN EXPRESSION; GENE-EXPRESSION; HISTIDINE PHOSPHORYLATION; DROSOPHILA DEVELOPMENT AB The nm23 genes were discovered on the basis of their reduced expression by highly metastatic cell lines. This trend was confirmed in cohorts of several types of human carcinomas and melanomas. Several transfection studies have demonstrated the suppressive effect of nm23 overexpression on the metastatic aggressiveness of melanoma and breast carcinoma cells in vivo. These transfection experiments have also demonstrated an effect of nm23 overexpression on cellular functions involved in the metastatic phenotype, such as cell motility, and point to a regulatory role for Nm23 proteins in cellular signalling pathways. Nm23 homologues from various species are also involved in normal tissue development and differentiation. Transfection of nm23-H1 into breast cancer cells provided a functional demonstration of the involvement of this gene in the differentiation of mammary epithelial cells. However, the molecular mechanism of these biological effects remains unknown. Several biochemical activities have been reported for Nm23, including NDP kinase activity, serine autophosphorylation and protein-histidine kinase activity. To define the possible significance of these biochemical activities, we carried out site-directed mutagenesis of the relevant codons of nm23-H1 cDNA and studied the effects upon transfection into MDA-MB-435 human breast carcinoma cells. We have also used Nm23 expression as a molecular marker to identify novel compounds that are active against the most aggressive tumour cells. This approach revealed that none of the standard agents currently in clinical use is preferentially active against the most aggressive tumour cells, and allowed us to identify new compounds that are preferentially inhibitory towards low-Nm23-expressing breast carcinoma and melanoma cell lines. This analysis also revealed a significant correlation between Nm23 levels and sensitivity of the tumour cells to alkylating agents. A functional implication of Nm23 proteins in this phenomenon was demonstrated after transfection of nm23 cDNAs into melanoma and breast and ovarian carcinoma cells. C1 NCI, Womens Canc Sect, Pathol Lab, Bethesda, MD 20892 USA. RP Freije, JMP (reprint author), NCI, Womens Canc Sect, Pathol Lab, Bldg 10,Rm 2A33, Bethesda, MD 20892 USA. RI Freije, Jose M.P./A-6535-2008 OI Freije, Jose M.P./0000-0002-4688-8266 NR 75 TC 47 Z9 47 U1 0 U2 1 PU PORTLAND PRESS LTD PI LONDON PA 59 PORTLAND PL, LONDON W1N 3AJ, ENGLAND SN 0067-8694 BN 1-85578-087-9 J9 BIOCHEM SOC SYMP PY 1998 IS 63 BP 261 EP 271 PG 11 WC Biochemistry & Molecular Biology; Oncology; Developmental Biology SC Biochemistry & Molecular Biology; Oncology; Developmental Biology GA BK13Y UT WOS:000071307400023 ER PT S AU Chen, YD Dougherty, ER AF Chen, YD Dougherty, ER BE Preteux, F Davidson, JL Dougherty, ER TI Adaptive design of logical structure filters SO MATHEMATICAL MODELING AND ESTIMATION TECHNIQUES IN COMPUTER VISION SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT Conference on Mathematical Modeling and Estimation Techniques in Computer Vision CY JUL 22-23, 1998 CL SAN DIEGO, CA SP SPIE Int Soc Opt Engn DE adaptive filter; optimal filter; granulometry; opening; mathematical morphology AB A binary granulometry is formed as a union of parameterized openings. It induces a reconstructive granulometry by passing image components it does not fully eliminate. Reconstructive granulometries have historically been formed as unions of reconstructive parameterized openings. The theory has been extended to the new class of logical structure filters (LSF) [1]. These are unions of intersections of both reconstructive and complementary reconstructive openings Reconstructive granulometries form the special class of disjunctive LSFs. This paper covers adaptive methods to design parameterized LSFs. A systematic formulation of adaptive transitions is given and applications are provided. C1 NIH, Canc Genet Lab, Natl Human Genome Res Inst, Bethesda, MD 20892 USA. RP Chen, YD (reprint author), NIH, Canc Genet Lab, Natl Human Genome Res Inst, Bldg 10, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPIE-INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA SN 0277-786X BN 0-8194-2912-0 J9 P SOC PHOTO-OPT INS PY 1998 VL 3457 BP 43 EP 51 DI 10.1117/12.323456 PG 9 WC Computer Science, Artificial Intelligence SC Computer Science GA BM20L UT WOS:000078025300004 ER PT S AU Greif, P Wastney, M Linares, O Boston, R AF Greif, P Wastney, M Linares, O Boston, R BE Clifford, AJ Muller, HG TI Balancing needs, efficiency, and functionality in the provision of modeling software: A perspective of the NIH WinSAAM Project. SO MATHEMATICAL MODELLING IN EXPERIMENTAL NUTRITION SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT Conference on Mathematical Modeling in Experimental Nutrition CY AUG 17-20, 1997 CL DAVIS, CALIFORNIA SP US Natl Inst Hlth, US Natl Sci Fdn, USDA, W Human Nutr Ctr, Lawrence Livermore Natl, Ctr Accelerator Mass Spect, F Hoffman La Roche & Co Ltd, Ralston Purina, Univ California Davis, Coll Agr & Environm Sci, Univ California Davis, Coll Letters & Sci, Div Math & Phys Sci ID MATHEMATICAL-MODEL; LACTATING COW; MAMMARY-GLAND; METABOLISM; SAAM; SIMULATION; NUTRIENTS; ENERGY; RUMEN; SHEEP AB The development of new software or the refinement of existing software for new operating environments each calls for judicious balancing. On the one hand, we strive for simplicity, predictability, and operational protection as it is well recognized that software with these attributes will attract an audience of satisfied users. But, on the other hand, these attributes do not conjure a sense of power, efficiency, or flexibility, and these other properties are also appreciated by users, albeit a somewhat different group of users. The goal is to achieve a blend which isolates critical functionality, flexible control, and user support while meeting the needs of the broadest collection of serious users. In this chapter, we discuss the issues impacting the migration of SAAM to the Windows environment, the NIH WinSAAM Project, and we outline the steps taken to ensure its feasibility. In addition, we describe a new paradigm for software development and use which ensures the durability of the software for modeling. C1 NIH, Lab Expt & Computat Biol, Washington, DC USA. RP Boston, R (reprint author), Univ Penn, New Bolton Ctr, 346 W St Rd, Kennett Square, PA 19348 USA. NR 28 TC 30 Z9 30 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46020-3 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 445 BP 3 EP 20 PG 18 WC Medicine, Research & Experimental; Nutrition & Dietetics SC Research & Experimental Medicine; Nutrition & Dietetics GA BL45M UT WOS:000075566800001 PM 9781379 ER PT J AU Kos, L Chiang, C Mahon, KA AF Kos, L Chiang, C Mahon, KA TI Mediolateral patterning of somites: multiple axial signals, including Sonic hedgehog, regulate Nkx-3.1 expression SO MECHANISMS OF DEVELOPMENT LA English DT Article DE somite; sclerotome; dermomyotome; neural tube; notochord; homeobox; myogenesis ID TERMINAL CLEAVAGE PRODUCT; MOTOR-NEURON INDUCTION; BHLH GENE-EXPRESSION; PROTEIN KINASE-A; FLOOR PLATE; MYOGENIC SPECIFICATION; SCLEROTOME INDUCTION; ECTOPIC EXPRESSION; CHICK-EMBRYO; NOTOCHORD AB The axial structures, the notochord and the neural tube, play an essential role in the dorsoventral patterning of somites and in the differentiation of their many cell lineages. Here, we investigated the role of the axial structures in the mediolateral patterning of the somite by using a newly identified murine homeobox gene, Nkr-3.1, as a medial semitic marker in explant in vitro assays. Nkx-3.1 is dynamically expressed during somitogenesis only in the youngest, most newly-formed somites at the caudal end of the embryo. We found that the expression of Nkx-3.1 in pre-semitic tissue explants is induced by the notochord and maintained in newly-differentiated somites by the notochord and both ventral and dorsal parts of the neural tube. We showed that Sonic hedgehog (Shh) is one of the signaling molecules that can reproduce the effect of the axial structures by exposing explants to either COS cells transfected with a Shh expression construct or to recombinant SHH. Shh could induce and maintain Nkx-3.1 expression in pre-semitic mesoderm and young somites but not in more mature, differentiated ones. The effects of Shh on Nkx-3.1 expression were antagonized by a forskolin-induced increase in the activity of cyclic AMP-dependent protein kinase A. Additionally, we confirmed that the expression of the earliest expressed murine myogenic marker, myf5, is also regulated by the axial strucutres but that Shh by itself is not capable of inducing or maintaining it. We suggest that the establishment of semitic medial and lateral compartments and the early events in myogenesis are governed by a combination of positive and inhibitory signals derived from the neighboring structures, as has previously been proposed for the dorsoventral patterning of somites. (C) 1998 Elsevier Science Ireland Ltd. C1 NICHD, Lab Mammalian Genes & Dev, NIH, Bethesda, MD 20892 USA. RP Kos, L (reprint author), NHGRI, Lab Genet Dis Res, NIH, 49 Convent Dr MSC 4472, Bethesda, MD 20892 USA. EM lidiak@nhgri.nih.gov NR 54 TC 42 Z9 42 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-4773 J9 MECH DEVELOP JI Mech. Dev. PD JAN PY 1998 VL 70 IS 1-2 BP 25 EP 34 DI 10.1016/S0925-4773(97)00168-8 PG 10 WC Developmental Biology SC Developmental Biology GA YY757 UT WOS:000072181300003 PM 9510022 ER PT J AU Nakayama, A Nguyen, MTT Chen, CC Opdecamp, K Hodgkinson, CA Amheiter, H AF Nakayama, A Nguyen, MTT Chen, CC Opdecamp, K Hodgkinson, CA Amheiter, H TI Mutations in microphthalmia, the mouse homolog of the human deafness gene MITF, affect neuroepithelial and neural crest-derived melanocytes differently SO MECHANISMS OF DEVELOPMENT LA English DT Article DE retinal pigment epithelium; Stria vascularis; basic-helix-loop-helix-zipper protein; DOPAchrome tautomerase; kit ID TYROSINE KINASE RECEPTOR; C-KIT; TRANSCRIPTION FACTOR; W-LOCUS; PROTO-ONCOGENE; VITILIGO MICE; MUTANT MICE; INNER-EAR; SI-LOCUS; IN-VITRO AB The mouse microphthalmia (Mitf) gene encodes a basic-helix-loop-helix-zipper transcription factor whose mutations are associated with abnormalities in neuroepithelial and neural crest-derived melanocytes. In wild type embryos, Mitf expression in neuropithelium and neural crest precedes that of the melanoblast marker Dct, is then co-expressed with Dct, and gradually fades away except in cells in hair follicles. In embryos with severe Mitf mutations, neural crest-derived Mitf-expressing cells are rare, lack Dct expression, and soon become undetectable. In contrast, the neuroepithelial-derived Mitf-expressing cells of the retinal pigment layer are retained, express Dct, but not the melanogenic enzyme genes tyrosinase and Tyr1, and remain unpigmented. The results show that melanocyte development critically depends on functional Mitf and that Mitf mutations affect the neural crest and the neuroepithelium in different ways. (C) 1998 Elsevier Science Ireland Ltd. C1 NINDS, Lab Dev Neurogenet, NIH, Bethesda, MD 20892 USA. RP Amheiter, H (reprint author), NINDS, Lab Dev Neurogenet, NIH, Bldg 36,Room 5D04,36 Convent Dr MSC 4160, Bethesda, MD 20892 USA. EM ha3p@nih.gov NR 45 TC 157 Z9 159 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-4773 J9 MECH DEVELOP JI Mech. Dev. PD JAN PY 1998 VL 70 IS 1-2 BP 155 EP 166 DI 10.1016/S0925-4773(97)00188-3 PG 12 WC Developmental Biology SC Developmental Biology GA YY757 UT WOS:000072181300013 PM 9510032 ER PT S AU Long, EO AF Long, EO BE Gupta, S Sher, A Ahmed, R TI Regulation of immune responses by inhibitory receptors SO MECHANISMS OF LYMPHOCYTE ACTIVATION AND IMMUNE REGULATION VII: MOLECULAR DETERMINANTS OF MICROBIAL IMMUNITY SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 7th International Conference on Lymphocyte Activation and Immune Regulation CY FEB 06-08, 1998 CL NEWPORT BEACH, CALIFORNIA ID NATURAL-KILLER-CELLS; CLASS-I MOLECULES; COMPLEX CLASS-I; TYROSINE-PHOSPHATASE 1C; HLA-C ALLOTYPES; NK CELLS; DIRECT BINDING; GENE-COMPLEX; AMINO-ACID; MAST-CELLS C1 NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. RP Long, EO (reprint author), NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. RI Long, Eric/G-5475-2011 OI Long, Eric/0000-0002-7793-3728 NR 54 TC 10 Z9 10 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46033-5 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 452 BP 19 EP 28 PG 10 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA BL84J UT WOS:000076911600003 PM 9889955 ER PT S AU Nelms, K Huang, H Ryan, J Keegan, A Paul, WE AF Nelms, K Huang, H Ryan, J Keegan, A Paul, WE BE Gupta, S Sher, A Ahmed, R TI Interleukin-4 receptor signalling mechanisms and their biological significance SO MECHANISMS OF LYMPHOCYTE ACTIVATION AND IMMUNE REGULATION VII: MOLECULAR DETERMINANTS OF MICROBIAL IMMUNITY SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 7th International Conference on Lymphocyte Activation and Immune Regulation CY FEB 06-08, 1998 CL NEWPORT BEACH, CALIFORNIA ID IL-4 RECEPTOR; CELLS; PROTEIN; STAT6; PHOSPHORYLATION; EXPRESSION; SUBSTRATE; PATHWAY; GROWTH; MICE C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. RP Nelms, K (reprint author), NIAID, Immunol Lab, NIH, Bldg 10, Bethesda, MD 20892 USA. NR 19 TC 33 Z9 33 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46033-5 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 452 BP 37 EP 43 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA BL84J UT WOS:000076911600005 PM 9889957 ER PT S AU Sher, A Hieny, S Charest, H Scharton-Kersten, T Collazo, C Germain, RN Sousa, CRE AF Sher, A Hieny, S Charest, H Scharton-Kersten, T Collazo, C Germain, RN Sousa, CRE BE Gupta, S Sher, A Ahmed, R TI The role of dendritic cells in the initiation of host resistance to Toxoplasma gondii SO MECHANISMS OF LYMPHOCYTE ACTIVATION AND IMMUNE REGULATION VII: MOLECULAR DETERMINANTS OF MICROBIAL IMMUNITY SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 7th International Conference on Lymphocyte Activation and Immune Regulation CY FEB 06-08, 1998 CL NEWPORT BEACH, CALIFORNIA ID TUMOR-NECROSIS-FACTOR; ENDOGENOUS IFN-GAMMA; ACUTE INFECTION; LYMPH-NODES; INTERLEUKIN-12; MICE; LIPOPOLYSACCHARIDE; INTERFERON; INDUCTION; PARASITE C1 NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. RP Sher, A (reprint author), NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. NR 23 TC 10 Z9 10 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46033-5 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 452 BP 103 EP 110 PG 8 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA BL84J UT WOS:000076911600012 PM 9889964 ER PT S AU Berger, EA AF Berger, EA BE Gupta, S Sher, A Ahmed, R TI HIV entry and tropism - When one receptor is not enough SO MECHANISMS OF LYMPHOCYTE ACTIVATION AND IMMUNE REGULATION VII: MOLECULAR DETERMINANTS OF MICROBIAL IMMUNITY SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 7th International Conference on Lymphocyte Activation and Immune Regulation CY FEB 06-08, 1998 CL NEWPORT BEACH, CALIFORNIA ID VIRUS TYPE-1 TROPISM; T-CELL LINES; CHEMOKINE RECEPTORS; FUNCTIONAL EXPRESSION; PRIMARY MACROPHAGES; FUSION COFACTORS; MIP-1-ALPHA; MIP-1-BETA; INFECTION; CLONING C1 NIAID, Viral Dis Lab, NIH, Bethesda, MD 20892 USA. RP Berger, EA (reprint author), NIAID, Viral Dis Lab, NIH, Bethesda, MD 20892 USA. NR 19 TC 17 Z9 17 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46033-5 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 452 BP 151 EP 157 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA BL84J UT WOS:000076911600016 PM 9889968 ER PT S AU Miller, LH Good, MF Kaslow, DC AF Miller, LH Good, MF Kaslow, DC BE Gupta, S Sher, A Ahmed, R TI Vaccines against the bloom stages of falciparum malaria SO MECHANISMS OF LYMPHOCYTE ACTIVATION AND IMMUNE REGULATION VII: MOLECULAR DETERMINANTS OF MICROBIAL IMMUNITY SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 7th International Conference on Lymphocyte Activation and Immune Regulation CY FEB 06-08, 1998 CL NEWPORT BEACH, CALIFORNIA ID MEROZOITE SURFACE PROTEIN-1; PLASMODIUM-FALCIPARUM; INFECTED ERYTHROCYTES; PROTECTIVE IMMUNITY; TERMINAL FRAGMENT; AOTUS MONKEYS; T-CELLS; ANTIGEN; IDENTIFICATION; TRANSMISSION C1 NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. RP Miller, LH (reprint author), NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. NR 45 TC 15 Z9 19 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46033-5 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 452 BP 193 EP 205 PG 13 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA BL84J UT WOS:000076911600022 PM 9889974 ER PT S AU Robbins, JB Schneerson, R Bryla, DA Trollfors, B Taranger, J Lagergard, T AF Robbins, JB Schneerson, R Bryla, DA Trollfors, B Taranger, J Lagergard, T BE Gupta, S Sher, A Ahmed, R TI Immunity to pertussis - Not all virulence factors are protective antigens SO MECHANISMS OF LYMPHOCYTE ACTIVATION AND IMMUNE REGULATION VII: MOLECULAR DETERMINANTS OF MICROBIAL IMMUNITY SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 7th International Conference on Lymphocyte Activation and Immune Regulation CY FEB 06-08, 1998 CL NEWPORT BEACH, CALIFORNIA ID BORDETELLA ADENYLATE-CYCLASE; LYMPHOCYTOSIS-PROMOTING FACTOR; OUTER-MEMBRANE PROTEIN; WHOLE-CELL VACCINE; FILAMENTOUS HEMAGGLUTININ; WHOOPING-COUGH; TOXOID VACCINE; SERUM ANTIBODIES; CONTROLLED TRIAL; PARAPERTUSSIS INFECTION C1 NICHHD, NIH, Bethesda, MD 20892 USA. RP Robbins, JB (reprint author), NICHHD, NIH, Bethesda, MD 20892 USA. NR 123 TC 2 Z9 2 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46033-5 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 452 BP 207 EP 218 PG 12 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA BL84J UT WOS:000076911600023 PM 9889975 ER PT S AU Epstein, ND AF Epstein, ND BE Sugi, H Pollack, GH TI The molecular biology and pathophysiology of hypertrophic cardiomyopathy due to mutations in the beta myosin heavy chains and the essential and regulatory light chains SO MECHANISMS OF WORK PRODUCTION AND WORK ABSORPTION IN MUSCLE SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT Symposium on Mechanisms of Work Production and Work Absorption in Muscle CY OCT 27-31, 1997 CL HAKONE, JAPAN SP Int Union Physiol Sci, Teikyo Univ, Uehara Sci Fdn, Kanagawa Sci Acad Fdn ID SKELETAL-MUSCLE AB Hypertrophic cardiomyopathy (HCM) is perhaps the most common cause of inherited sudden death in otherwise healthy young individuals. There are presently seven known genes in which mutations have been shown to cause the disease. The first identified disease gene was beta myosin heavy chain (BMHC). Our laboratory has identified 32 distinct BMHC gene mutations in 62 kindreds after screening representatives of over 400 kindreds. Virtually all but one of approximately 50 known mutations are restricted to the head or head-rod junction region of the molecule. We have used the mutant alleles of the BMHC gene to demonstrate that both mutant message and protein is present in the skeletal muscle of patients with HCM. Muscle biopsies from patients with identified BMHC mutations show abnormal histology. Isolated myosin and skinned fibers from these patients have abnormal mechanical properties. The BMHC gene mutations are clustered in 4 regions of the myosin head. Because one of these regions is adjacent to the ELC, we scanned HCM patient DNA for mutations in either the ELC or RLC. Linkage analysis showed that a unique mutation in the ELC caused a rare phenotype of HCM in one family. Other mutations in either light chain were also associated with the same rare phenotype in other families. Through several lines of reasoning we hypothesized that the light chain mutations interfere with the stretch-activation response of papillary muscle and adjacent ventricular tissue. This properly is critical to oscillatory power output of insect flight muscle. We conjectured that this properly is also exploited by portions of the heart to increase power output. in order to test this hypothesis we constructed transgenic mouse lines expressing either the human normal or mutant ELC. The cardiac morphology and mechanical properties of the transgenic mouse papillary muscle is now being studied. C1 NHLBI, Cardiol Branch, NIH, Bethesda, MD 20892 USA. RP Epstein, ND (reprint author), NHLBI, Cardiol Branch, NIH, Bldg 10, Bethesda, MD 20892 USA. NR 20 TC 19 Z9 20 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46037-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 453 BP 105 EP 115 PG 11 WC Cell Biology; Medicine, Research & Experimental; Physiology SC Cell Biology; Research & Experimental Medicine; Physiology GA BM32K UT WOS:000078390400013 PM 9889820 ER PT S AU Xu, S Malinchik, S Frisbie, S Gu, J Kraft, T Rapp, G Chalovich, JM Brenner, B Yu, LC AF Xu, S Malinchik, S Frisbie, S Gu, J Kraft, T Rapp, G Chalovich, JM Brenner, B Yu, LC BE Sugi, H Pollack, GH TI X-ray diffraction studies of the cross-bridge intermediate states SO MECHANISMS OF WORK PRODUCTION AND WORK ABSORPTION IN MUSCLE SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT Symposium on Mechanisms of Work Production and Work Absorption in Muscle CY OCT 27-31, 1997 CL HAKONE, JAPAN SP Int Union Physiol Sci, Teikyo Univ, Uehara Sci Fdn, Kanagawa Sci Acad Fdn ID RABBIT PSOAS MUSCLE; RELAXED FIBER STIFFNESS; FORCE GENERATION; PARALLEL INHIBITION; STRUCTURAL-CHANGES; SKELETAL-MUSCLE; IONIC-STRENGTH; SKINNED FIBERS; ACTIVE FORCE; ACTOMYOSIN AB Two dimensional x-ray diffraction was obtained from skinned rabbit psoas muscle fibers. The goal is to correlate structures of the cross-bridge population with various intermediate states in the cross-bridge cycle by using nucleotides and their analogs. It was found that in a relaxed muscle in ATP containing solutions, cross-bridges are distributed in three populations in equilibrium: those detached from actin and ordered on the myosin helix, those that are detached and disordered, and those weakly attached to actin in random orientations. The distribution among the three populations is highly dependent on temperature. Those that are detached and yet ordered in a helical structure surrounding the myosin backbone are very likely in the M.ADP.P-i state, supporting an earlier suggestion by Wray (1987). It was also found that the attached cross-bridges with bound MgADP are structurally distinct from those without nucleotide, in agreement with one of our earlier findings by osmotic compression (Xu et al., 1993). Another finding of interest is that the analog AMP-PNP was found to be an ATP analog, rather than an ADP analog as it has been reported previously by many research groups. C1 NIH, Bethesda, MD 20892 USA. DESY, European Mol Biol Lab, Hamburg Outstn, D-2000 Hamburg, Germany. E Carolina Med Sch, Greenville, NC USA. Hannover Med Sch, D-3000 Hannover, Germany. RP Xu, S (reprint author), NIH, Bldg 10, Bethesda, MD 20892 USA. OI Chalovich, Joseph/0000-0002-1243-4055 FU NIAMS NIH HHS [R01 AR 44504] NR 20 TC 1 Z9 1 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46037-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 453 BP 271 EP 279 PG 9 WC Cell Biology; Medicine, Research & Experimental; Physiology SC Cell Biology; Research & Experimental Medicine; Physiology GA BM32K UT WOS:000078390400032 PM 9889839 ER PT S AU Davis, JS AF Davis, JS BE Sugi, H Pollack, GH TI Force generation simplified - Insights from laser temperature-jump experiments on contracting muscle fibers SO MECHANISMS OF WORK PRODUCTION AND WORK ABSORPTION IN MUSCLE SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT Symposium on Mechanisms of Work Production and Work Absorption in Muscle CY OCT 27-31, 1997 CL HAKONE, JAPAN SP Int Union Physiol Sci, Teikyo Univ, Uehara Sci Fdn, Kanagawa Sci Acad Fdn ID TENSION TRANSIENTS; STRIATED-MUSCLE; MYOSIN; PHOTOGENERATION; TRANSITION; RESPONSES; PHOSPHATE; RELEASE AB Raising the temperature of a maximally Ca2+-activated muscle fiber causes a sigmoidal increase in tension. The kinetics that govern this process can be explored by step-heating the fiber a few degrees with a laser temperature-jump. A biexponential increase in tension results; a third exponential phase that opposes this biphasic rise in tension is only observed when phosphate, a reaction product normally at low concentration, is added to the fiber. This chapter explains how the temperature dependencies of isometric tension and the temperature jump kinetics interrelate; and how these insights have modified and simplified our understanding of current mechanisms of force generation. The fast kinetic phase of the tension rise appears associated with single-step force generation or a power stroke, a process largely isolated from adjacent steps in the crossbridge cycle. The amplitude of the slow phase of the tension rise exhibits a remarkable similar to 1:1 ratio to the amplitude of the fast, tension generating phase above 10 degrees C. The similarity of these two amplitudes, that combine to give the complete rise in isometric tension with temperature, appear to fit a model in which one of a pair of myosin heads generates force while the second head is poised to function after the power stroke of the first has occurred. The phase with the negative amplitude seen with added phosphate points to a mechanism in which phosphate release is indirectly linked to the tension generation by forward flow through the crossbridge cycle to tension generation. C1 NHLBI, NIH, Bethesda, MD 20892 USA. RP Davis, JS (reprint author), NHLBI, NIH, Bldg 10, Bethesda, MD 20892 USA. NR 19 TC 18 Z9 18 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46037-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 453 BP 343 EP 352 PG 10 WC Cell Biology; Medicine, Research & Experimental; Physiology SC Cell Biology; Research & Experimental Medicine; Physiology GA BM32K UT WOS:000078390400039 PM 9889846 ER PT S AU Schoenberg, M AF Schoenberg, M BE Sugi, H Pollack, GH TI Crossbridge head detachment rate constants determined from a model that explains the behavior of both weakly- and strongly-binding crossbridges SO MECHANISMS OF WORK PRODUCTION AND WORK ABSORPTION IN MUSCLE SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT Symposium on Mechanisms of Work Production and Work Absorption in Muscle CY OCT 27-31, 1997 CL HAKONE, JAPAN SP Int Union Physiol Sci, Teikyo Univ, Uehara Sci Fdn, Kanagawa Sci Acad Fdn ID CROSS-BRIDGE BEHAVIOR; RABBIT PSOAS FIBERS; MUSCLE-FIBERS; MAGNESIUM PYROPHOSPHATE; THEORETICAL CONSIDERATIONS; ACTOMYOSIN SUBFRAGMENT-1; MYOSIN SUBFRAGMENT-1; ACTIN; ATP; COMPLEX AB Experimentally it is observed that the head regions of weakly-binding myosin crossbridges (crossbridges with ATP or ADP.P-i at the nucleotide binding site) are mobile while attached to actin, while strongly-binding crossbridge heads, such as those with PPi or AMP-PNP at the nucleotide binding site, are immobile (Pate and Cooke, Biophys. J., 1988; Fajer et al., Biophys. J., 1988). I postulate that the fundamental difference between weakly- and strongly-binding crossbridges is not their difference in affinity for actin, but the difference in mobility of the myosin heads attached to actin. Because the heads of weakly-binding crossbridges are mobile while attached to actin, the heads function independently and their behavior can be described by a simple independent-head model. With strongly-binding crossbridges, when one head detaches, it cannot re-attach in a position of lesser strain while the other is attached immobile; both heads must be detached concurrently before the crossbridge can relocate to a position of less strain and relax any tension it supports. This makes the heads appear to act cooperatively. A double-headed crossbridge model is presented which takes into account the difference between weakly- and strongly-binding crossbridges. The model is quite successful at describing the experimental data. In particular, for weakly-binding crossbridges the time constant of the response to stretch is shown to be relatively insensitive to ionic strength and for strongly-binding crossbridges, the model predicts with great accuracy the large ionic strength dependence of the rate constant for force decay. When the experimental results are interpreted according to the model, an important conclusion that emerges is that in ail cases (for both weakly- and stronely-binding crossbridges) unstrained crossbridge heads in the muscle fiber detach from actin with approximately the same rate constant as myosin subfragment-1 detaches from actin in solution. C1 NIAMSD, Phys Biol Lab, NIH, Bethesda, MD 20892 USA. RP Schoenberg, M (reprint author), NIAMSD, Phys Biol Lab, NIH, Bethesda, MD 20892 USA. NR 21 TC 3 Z9 3 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46037-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 453 BP 425 EP 434 PG 10 WC Cell Biology; Medicine, Research & Experimental; Physiology SC Cell Biology; Research & Experimental Medicine; Physiology GA BM32K UT WOS:000078390400047 PM 9889854 ER PT J AU Gelderman, MP Stuart, R Vigerust, D Fuhrmann, S Lefkowitz, DL Allen, RC Lefkowitz, SS Graham, S AF Gelderman, MP Stuart, R Vigerust, D Fuhrmann, S Lefkowitz, DL Allen, RC Lefkowitz, SS Graham, S TI Perpetuation of inflammation associated with experimental arthritis: the role of macrophage activation by neutrophilic myeloperoxidase SO MEDIATORS OF INFLAMMATION LA English DT Article DE autoimmunity; peroxidases; TNF-alpha; rheumatoid arthritis; reactive oxygen intermediates ID NECROSIS-FACTOR-ALPHA; RHEUMATOID-ARTHRITIS; MANNOSE RECEPTOR; SYNOVIAL-FLUID; PHAGOCYTOSIS; INACTIVATION; CYTOKINES; MICE AB RHEUMATOID arthritis (RA) is characterized by an abnormal cellular and cytokine infiltration of inflamed joints. This study addresses a previously unrecognized interaction between neutrophilic-myeloperoxidase (MPO) and macrophages (Mo) which could explain the perpetuation of inflammation associated with RA. A monoarticular arthritis was induced in female Lewis rats by injection of streptococcal cell wall extracts (PG-APS). After swelling and erythema subsided, joints were re-injected with one of the following: porcine MPO or partially inactivated MPO (iMPO). injection with either MPO or iMPO induced a 'flare' of experimental RA. Blocking the Mpr-mannose receptor by mannans, ablated exacerbation of disease. These results indicate that MPO or iMPO can play a pivotal role in the perpetuation but not initiation of this RA model. C1 Texas Tech Univ, Lubbock, TX 79409 USA. NIH, Bethesda, MD 20931 USA. Severance & Associates PA, San Antonio, TX 78207 USA. Texas Tech Univ, Hlth Sci Ctr, Lubbock, TX 79430 USA. RP Lefkowitz, DL (reprint author), Texas Tech Univ, Lubbock, TX 79409 USA. RI Allen, Robert/B-6529-2008; Vigerust, David/B-5336-2016 OI Vigerust, David/0000-0002-8884-2936 NR 37 TC 15 Z9 15 U1 0 U2 1 PU CARFAX PUBLISHING PI BASINGSTOKE PA RANKINE RD, BASINGSTOKE RG24 8PR, HANTS, ENGLAND SN 0962-9351 J9 MEDIAT INFLAMM JI Mediat. Inflamm. PY 1998 VL 7 IS 6 BP 381 EP 389 PG 9 WC Cell Biology; Immunology SC Cell Biology; Immunology GA 157ZX UT WOS:000078092500002 PM 9927230 ER PT S AU Xu, CY Pham, DL Prince, JL Etemad, ME Yu, DN AF Xu, CY Pham, DL Prince, JL Etemad, ME Yu, DN BE Wells, WM Colchester, A Delp, S TI Reconstruction of the central layer of the human cerebral cortex from MR images SO MEDICAL IMAGE COMPUTING AND COMPUTER-ASSISTED INTERVENTION - MICCAI'98 SE Lecture Notes in Computer Science LA English DT Article; Proceedings Paper CT 1st International Conference on Medical Image Computing and Computer-Assisted Intervention (MICCAI 98) CY OCT 11-13, 1998 CL CAMBRIDGE, MA SP Harvard Med Sch, Boston, MIT, MA ID ACTIVE CONTOUR MODELS; BALLOONS AB Reconstruction of the human cerebral cortex from MR images is a fundamental step in human brain mapping and in applications such as surgical path planning. In a previous paper, we described a method for obtaining a surface representation of the central layer of the human cerebral cortex using fuzzy segmentation and a deformable surface model. This method, however, suffers from several problems. In this paper, we significantly improve upon the previous method by using a fuzzy segmentation algorithm robust to intensity inhomogeneities, and using a deformable surface model specifically designed for capturing convoluted sulci or gyri. We demonstrate the improvement over the previous method both qualitatively and quantitatively, and show the result of its application to six subjects. We also experimentally validate the convergence of the deformable surface initialization algorithm. C1 Johns Hopkins Univ, Baltimore, MD 21218 USA. NIA, Lab Personal & Cognit, GRC, NIH, Baltimore, MD 21214 USA. RP Johns Hopkins Univ, Baltimore, MD 21218 USA. RI Prince, Jerry/A-3281-2010 OI Prince, Jerry/0000-0002-6553-0876 NR 13 TC 4 Z9 4 U1 0 U2 0 PU SPRINGER-VERLAG BERLIN PI BERLIN PA HEIDELBERGER PLATZ 3, D-14197 BERLIN, GERMANY SN 0302-9743 BN 3-540-65136-5 J9 LECT NOTES COMPUT SC PY 1998 VL 1496 BP 481 EP 488 PG 8 WC Computer Science, Interdisciplinary Applications; Computer Science, Theory & Methods; Engineering, Biomedical; Medical Laboratory Technology; Clinical Neurology; Neurosciences; Radiology, Nuclear Medicine & Medical Imaging; Surgery SC Computer Science; Engineering; Medical Laboratory Technology; Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging; Surgery GA BN52N UT WOS:000082115900052 ER PT S AU Saxena, P Pavel, DG Quintana, JC Horwitz, B AF Saxena, P Pavel, DG Quintana, JC Horwitz, B BE Wells, WM Colchester, A Delp, S TI An automatic threshold-based seating method for enhancing the usefulness of Tc-HMPAO SPECT in the diagnosis of Alzheimer's disease SO MEDICAL IMAGE COMPUTING AND COMPUTER-ASSISTED INTERVENTION - MICCAI'98 SE LECTURE NOTES IN COMPUTER SCIENCE LA English DT Article; Proceedings Paper CT 1st International Conference on Medical Image Computing and Computer-Assisted Intervention (MICCAI 98) CY OCT 11-13, 1998 CL CAMBRIDGE, MASSACHUSETTS SP Harvard Med Sch, Boston, Massachusetts Inst Technol, MA ID ACCURATE; DEMENTIA; ATROPHY; MRI AB Functional imaging of the brain can aid in the diagnosis of Alzheimer's disease. Tc-HMPAO SPECT is widely available and relatively inexpensive to use. Combined with computer-based analysis of images, SPECT is a powerful tool in detecting decreases in brain perfusion caused by Alzheimer's disease. However, analysis can falsely elevate the perfusion of normal areas and diminish the perfusion of atrophic areas in the Alzheimer's brain when used with conventional scaling methods. In this paper, we present a technique for scaling images that overcomes the problems associated with conventional scaling methods. Our technique was successful in eliminating or attenuating the false increases in perfusion shown in probable Alzheimer's patients in over 90% of cases (n=17), and in enhancing the sensitivity of detection of degenerative changes by Statistical Parametric Mapping. C1 Univ Illinois, Dept Neurosurg, Biomed Visualizat Lab, Chicago, IL 60612 USA. Univ Illinois, Dept Radiol, Chicago, IL 60612 USA. NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. Catholic Univ Chile, Santiago, Chile. RP Saxena, P (reprint author), Univ Illinois, Dept Neurosurg, Biomed Visualizat Lab, 912 S Wood St, Chicago, IL 60612 USA. NR 19 TC 36 Z9 36 U1 1 U2 1 PU SPRINGER-VERLAG BERLIN PI BERLIN PA HEIDELBERGER PLATZ 3, D-14197 BERLIN, GERMANY SN 0302-9743 BN 3-540-65136-5 J9 LECT NOTES COMPUT SC PY 1998 VL 1496 BP 623 EP 630 PG 8 WC Computer Science, Interdisciplinary Applications; Computer Science, Theory & Methods; Engineering, Biomedical; Medical Laboratory Technology; Clinical Neurology; Neurosciences; Radiology, Nuclear Medicine & Medical Imaging; Surgery SC Computer Science; Engineering; Medical Laboratory Technology; Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging; Surgery GA BN52N UT WOS:000082115900067 ER PT S AU Summers, RM Pusanik, LM Malley, JD AF Summers, RM Pusanik, LM Malley, JD BE Hanson, KM TI Automatic detection of endobronchial lesions with virtual bronchoscopy: comparison of two methods SO MEDICAL IMAGING 1998: IMAGE PROCESSING, PTS 1 AND 2 SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT Medical Imaging 1998 Conference on Image Processing CY FEB 23-26, 1998 CL SAN DIEGO, CA SP SPIE, Amer Assoc Physicists Med, Amer Physiol Soc, FDA Ctr Devices & Radiat Hlth, Soc Imaging Sci & Technol, Natl Elect Manufacturers Assoc, Diagnost Imaging & Therapy Syst Div, Radiol Informat Syst Consortium, Radiol Soc N Amer, Soc Comp Applicat Radiol DE computed tomography; virtual bronchoscopy; computer-aided diagnosis; bronchi; trachea; curvature; three-dimensional reconstruction AB Three-dimensional reconstruction of medical images is increasingly being used to diagnose disease and to direct therapy. Virtual bronchoscopy is a recently developed type of three-dimensional reconstruction of the airways that may be useful for diagnosis of lesions of the airway. In this study, we compare two methods for computer-aided diagnosis of polypoid airway tumors: a parametric ("patch") and non-parametric ("grey-scale") algorithm. We found that both methods have comparable specificities. Although the non-parametric method is twelve times faster than the parametric method, we found that its sensitivity lags behind that of the parametric method by 3 to 16% when lesions of all sizes are considered. For lesions at least 5 mm in size, the sensitivities are comparable if a small convolution kernel is used. C1 NIH, Warren G Magnuson Clin Ctr, Dept Diagnost Radiol, Bethesda, MD 20892 USA. RP Summers, RM (reprint author), NIH, Warren G Magnuson Clin Ctr, Dept Diagnost Radiol, Bethesda, MD 20892 USA. NR 0 TC 11 Z9 11 U1 0 U2 1 PU SPIE-INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA SN 0277-786X BN 0-8194-2783-7 J9 P SOC PHOTO-OPT INS PY 1998 VL 3338 BP 327 EP 335 DI 10.1117/12.310909 PN 1 & 2 PG 9 WC Engineering, Biomedical; Optics; Radiology, Nuclear Medicine & Medical Imaging SC Engineering; Optics; Radiology, Nuclear Medicine & Medical Imaging GA BL38V UT WOS:000075346500032 ER PT S AU Long, LR Goh, GH Neve, L Thoma, GR AF Long, LR Goh, GH Neve, L Thoma, GR BE Horii, SC Blaine, GJ TI World wide web platform-independent access to biomedical text/image databases SO MEDICAL IMAGING 1998 - PACS DESIGN AND EVALUATION: ENGINEERING AND CLINICAL ISSUES SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT PACS Design and Evaluation Conference CY FEB 24-26, 1998 CL SAN DIEGO, CA SP SPIE, Amer Assoc Physicists Med, Amer Physiol Soc, US FDA, Ctr Devices & Radiol Hlth, Soc Imaging Sci & Technol, Natl Elect Mfg Assoc, Diag Imaging & Therapy Syst Div, Radiol Informat Syst Consortium, Radiol Soc N Amer, Soc Comp Applicat Radiol DE World Wide Web; biomedical; informatics; Internet; database; spine; digital image; morphometry; x-ray image; image query by content; NHANES; NLM; NCHS; NIAMS AB The biomedical digital library of the future is expected to provide access to stores of biomedical database information containing text and images. Developing efficient methods for accessing such databases is a research effort at the Lister Hill National Center for Biomedical Communications of the National Library of Medicine. In this paper we examine issues in providing access to databases across the Web and describe a tool we have developed: the Web-based Medical Information Retrieval System (WebMIRS). We address a number of critical issues, including preservation of data integrity, efficient database design, access to documentation, quality of query and results interfaces, capability to export results to other software, and exploitation of multimedia data. WebMIRS is implemented as a Java applet that allows database access to text and to associated image data, without requiring any user software beyond a standard Web browser. The applet implementation allows WebMIRS to run on any hardware platform (such as PCs, the Macintosh, or Unix machines) which supports a Java-enabled Web browser, such as Netscape or Internet Explorer. WebMIRS is being tested on text/x-ray image databases created from the National Health and Nutrition Examination Surveys (NHANES) data collected by the National Center for Health Statistics. C1 Natl Lib Med, Bethesda, MD 20894 USA. RP Long, LR (reprint author), Natl Lib Med, Bethesda, MD 20894 USA. NR 0 TC 4 Z9 4 U1 0 U2 0 PU SPIE-INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA SN 0277-786X BN 0-8194-2784-5 J9 P SOC PHOTO-OPT INS PY 1998 VL 3339 BP 52 EP 63 DI 10.1117/12.319807 PG 12 WC Engineering, Biomedical; Medical Informatics; Optics; Radiology, Nuclear Medicine & Medical Imaging SC Engineering; Medical Informatics; Optics; Radiology, Nuclear Medicine & Medical Imaging GA BL49R UT WOS:000075701600006 ER PT J AU Newman, AH AF Newman, AH TI Novel dopamine transporter ligands: The state of the art SO MEDICINAL CHEMISTRY RESEARCH LA English DT Editorial Material ID BINDING DOMAINS; NOREPINEPHRINE TRANSPORTERS; COCAINE BINDING; ANALOGS; REINFORCEMENT; INHIBITION; ADDICTION; SUBSTRATE; PIMOZIDE; BLOCKADE AB The dopamine transporter has served as the primary molecular target for the development of potential pharmacotherapeutic treatments for cocaine-abuse. The evolution of structure-activity relationships, within various classes of dopamine uptake inhibitors, has provided selective molecular probes to study the dopamine transporter. Ultimately, the elucidation of structure and function of this protein will undoubtedly provide new leads toward treatment of cocaine addiction. C1 NIDA, Psychobiol Sect, Intramural Res Program, NIH, Baltimore, MD 21224 USA. RP Newman, AH (reprint author), NIDA, Psychobiol Sect, Intramural Res Program, NIH, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. NR 41 TC 35 Z9 35 U1 1 U2 1 PU BIRKHAUSER BOSTON INC PI CAMBRIDGE PA 675 MASSACHUSETTS AVE, CAMBRIDGE, MA 02139 USA SN 1054-2523 J9 MED CHEM RES JI Med. Chem. Res. PY 1998 VL 8 IS 1-2 BP 1 EP 11 PG 11 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA ZQ628 UT WOS:000073887100001 ER PT J AU Lomenzo, SA Izenwasser, S Katz, JL Trudell, ML AF Lomenzo, SA Izenwasser, S Katz, JL Trudell, ML TI The effects of alkyl substituents at the 6-position of cocaine analogues on dopamine transporter binding affinity and dopamine uptake inhibition SO MEDICINAL CHEMISTRY RESEARCH LA English DT Article ID LIGAND-BINDING; SEROTONIN; SITES; DISCOVERY; RECEPTOR; POTENT AB A series of 6-alkyl-3 beta-benzyl-2-(methoxycarbonylmethyl)tropane analogues were synthesized and evaluated as cocaine binding-site ligands at the dopamine transporter (DAT). The in vitro inhibition of [3H]dopamine and the DAT binding affinity (K-i, [H-3]WIN 35,428) were measured in rat caudate putamen tissue. Generally, substitution at the B-position was found to reduce ligand potency. C1 Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. Univ Miami, Sch Med, Dept Neurol, Miami, FL 33136 USA. NIDA, Div Intramural Res, Baltimore, MD 21224 USA. RP Lomenzo, SA (reprint author), Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. RI Izenwasser, Sari/G-9193-2012; OI Katz, Jonathan/0000-0002-1068-1159 NR 33 TC 4 Z9 4 U1 0 U2 0 PU BIRKHAUSER BOSTON INC PI CAMBRIDGE PA 675 MASSACHUSETTS AVE, CAMBRIDGE, MA 02139 USA SN 1054-2523 J9 MED CHEM RES JI Med. Chem. Res. PY 1998 VL 8 IS 1-2 BP 35 EP 42 PG 8 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA ZQ628 UT WOS:000073887100003 ER PT J AU Zhang, Y AF Zhang, Y TI The identification of GBR 12909 as a potential therapeutic agent for cocaine abuse SO MEDICINAL CHEMISTRY RESEARCH LA English DT Article ID DOPAMINE REUPTAKE INHIBITORS; DRUG DEVELOPMENT; TRANSPORTER; ANALOGS; GBR-12909; BINDING; ANTAGONISTS; RECEPTORS; AFFINITY; RAT AB Current studies on the treatment of cocaine abuse have focused on the development of high affinity and selective dopamine transporter ligands as dopamine reuptake inhibitors. Among the various classes of ligands identified, the disubstituted piperazine GBR 12909, has demonstrated high potency and selectivity for the transporter. This article reviews the identification of GBR 12909 as a high affinity dopamine reuptake inhibitor, the SAR studies on GBR 12909 performed to date, the behavioral studies of this compound in animal models, and the development of long-acting formulation of GBR 12909 as a potential therapeutic agent for the treatment of the cocaine abuse. C1 NIDDKD, Med Chem Lab, NIH, Bethesda, MD 20892 USA. RP Zhang, Y (reprint author), NIDDKD, Med Chem Lab, NIH, 8 Ctr Dr,MSC 0815, Bethesda, MD 20892 USA. NR 44 TC 11 Z9 11 U1 1 U2 1 PU BIRKHAUSER BOSTON INC PI CAMBRIDGE PA 675 MASSACHUSETTS AVE, CAMBRIDGE, MA 02139 USA SN 1054-2523 J9 MED CHEM RES JI Med. Chem. Res. PY 1998 VL 8 IS 1-2 BP 66 EP 76 PG 11 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA ZQ628 UT WOS:000073887100006 ER PT J AU Houlihan, WJ Boja, JW Kopajtic, TA Kuhar, MJ Degrado, SJ Toledo, L AF Houlihan, WJ Boja, JW Kopajtic, TA Kuhar, MJ Degrado, SJ Toledo, L TI Positional isomers and analogs of mazindol as potential inhibitors of the cocaine binding site on the dopamine transporter site SO MEDICINAL CHEMISTRY RESEARCH LA English DT Article ID AMPHETAMINE AB A series of compounds, where the keto-tautomeric form of mazindol (2b) was modified by placing the 2-(p-chlorobenzoyl) portion of the molecule in the 3- and 4-positions, substituting the imidazo ring A by a 4,4-dimethyl 1-2-oxazolo ring, and replacing the carbonyl group by a CHOH, CH2, O, S, SO and SO2 were prepared and evaluated for their ability to displace [H-3] WIN 35,428 binding in rat striatal tissue. All compounds were weaker (24-2,050-fold) than mazindol. C1 Drew Univ, Charles A Dana Res Inst, Madison, NJ 07940 USA. NIDA, Addict Res Ctr, Baltimore, MD 21224 USA. RP Houlihan, WJ (reprint author), Drew Univ, Charles A Dana Res Inst, Hall Sci, Madison, NJ 07940 USA. NR 27 TC 10 Z9 10 U1 0 U2 0 PU BIRKHAUSER BOSTON INC PI CAMBRIDGE PA 675 MASSACHUSETTS AVE, CAMBRIDGE, MA 02139 USA SN 1054-2523 J9 MED CHEM RES JI Med. Chem. Res. PY 1998 VL 8 IS 1-2 BP 77 EP 90 PG 14 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA ZQ628 UT WOS:000073887100007 ER PT J AU May, EL Jacobson, AE Mattson, MV Coop, A Aceto, MD Bowman, ER Traynor, JR Woods, JH Harris, LS AF May, EL Jacobson, AE Mattson, MV Coop, A Aceto, MD Bowman, ER Traynor, JR Woods, JH Harris, LS TI Synthesis, in vitro, and in vivo activity of N-p-substitutedbenzyl- (-)- and (+)-N-normetazocines, (-)- and (+)-N-normorphinans, N-norketobemidones, and (-)-N-nor-4,5-epoxymorphinans SO MEDICINAL CHEMISTRY RESEARCH LA English DT Article ID GUINEA-PIG BRAIN; SIGMA-RECEPTORS; BINDING-SITES; VAS-DEFERENS; RAT-BRAIN; AFFINITY; POTENT; LIGAND; CLASSIFICATION; PHENCYCLIDINE AB N-benzyl- and N-p-nitrobenzyl-N-norketobemidone, and N-p-fluorobenzyl-N-normetazocine have been synthesized and found to have equivalent or higher affinity for the sigma(1)-binding site than the previously described (+)-N-benzylnormetazocine. None of the examined compounds had significant affinity for the sigma(2)-binding site and few had high affinity for mu-, delta, and kappa-opioid receptors. (-)-N-Benzyl-N-normetazocine displayed weak agonist-antagonist activity and (-)-N-benzylnoroxymorphone had one-tenth the antagonist activity of naloxone. C1 Virginia Commonwealth Univ, Med Coll Virginia, Dept Pharmacol, Richmond, VA 23298 USA. Univ Michigan, Sch Med, Dept Pharmacol, Ann Arbor, MI 48109 USA. NIDDK, Med Chem Lab, NIH, Bethesda, MD 20892 USA. RP May, EL (reprint author), Virginia Commonwealth Univ, Med Coll Virginia, Dept Pharmacol, Richmond, VA 23298 USA. NR 34 TC 13 Z9 13 U1 0 U2 0 PU BIRKHAUSER BOSTON INC PI CAMBRIDGE PA 675 MASSACHUSETTS AVE, CAMBRIDGE, MA 02139 USA SN 1054-2523 J9 MED CHEM RES JI Med. Chem. Res. PY 1998 VL 8 IS 6 BP 311 EP 321 PG 11 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA 140XY UT WOS:000077097000004 ER PT J AU Bar-Or, O Foreyt, J Bouchard, C Brownell, KD Dietz, WH Ravussin, E Salbe, AD Schwenger, S St Jeor, S Torun, B AF Bar-Or, O Foreyt, J Bouchard, C Brownell, KD Dietz, WH Ravussin, E Salbe, AD Schwenger, S St Jeor, S Torun, B TI Physical activity, genetic, and nutritional considerations in childhood weight management SO MEDICINE AND SCIENCE IN SPORTS AND EXERCISE LA English DT Editorial Material DE behavior modification; diet; energy expenditure; exercise; heredity; obesity; prevention; public policy; TV viewing; undernutrition ID DOUBLY-LABELED WATER; TOTAL-ENERGY-EXPENDITURE; OBESE CHILDREN; AEROBIC EXERCISE; BODY FATNESS; OVERWEIGHT; ADOLESCENTS; HEALTH; RISK; AGE AB Juvenile obesity is a serious, increasingly prevalent problem in technologically developed societies. Almost one-quarter of U.S. children are now obese, a dramatic increase of over 20% in the past decade. It is intriguing that the increase in prevalence has been occurring while overall fat consumption has been declining. Body mass and composition are influenced by genetic factors, but the actual heritability of juvenile obesity is not known. A low physical activity (PA) is characteristic of obese children and adolescents, and it may be one cause of juvenile obesity. There is little evidence, however, that overall energy expenditure is low among the obese. There is a strong association between the prevalence of obesity and the extent of TV viewing. Enhanced PA can reduce body fat and blood pressure and improve lipoprotein profile in obese individuals. Its effect on body composition, however, is slower than with low-calorie diets. The three main dietary approaches are: protein sparing modified fast, balanced hypocaloric diets, and comprehensive behavioral lifestyle programs. To achieve long-standing control of overweight, one should combine changes in eating and activity patterns, using behavior modification techniques. However, the onus is also on society to reduce incentives for a sedentary lifestyle and over-consumption of food. To address the key issues related to childhood weight management, the American College of Sports Medicine convened a Scientific Roundtable in Indianapolis. C1 McMaster Univ, Chedoke Hosp Div, Childrens Exercise & Nutr Ctr, Hamilton, ON L8N 3Z5, Canada. Baylor Coll Med, Dept Med, Houston, TX 77030 USA. Baylor Coll Med, Dept Psychiat, Houston, TX 77030 USA. Univ Laval, Phys Act Sci Lab, Ste Foy, PQ G1K 7P4, Canada. Yale Univ, Yale Ctr Eating & Weight Disorders, New Haven, CT USA. Tufts Univ, Dept Pediat, Boston, MA 02111 USA. NIDDKD, Clin Diabet & Nutr Sect, NIH, Phoenix, AZ 85016 USA. Univ Nevada, Nutr Educ & Res Program, Reno, NV 89557 USA. Inst Nutr Cent Amer & Panama, Human Nutr Unit, Guatemala City, Guatemala. RP Bar-Or, O (reprint author), McMaster Univ, Chedoke Hosp Div, Childrens Exercise & Nutr Ctr, Hamilton, ON L8N 3Z5, Canada. EM baror@fhs.mcmaster.ca RI Biguzzi, Felipe/E-4724-2015; Bouchard, Claude/A-7637-2009 NR 67 TC 118 Z9 126 U1 3 U2 11 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0195-9131 J9 MED SCI SPORT EXER JI Med. Sci. Sports Exerc. PD JAN PY 1998 VL 30 IS 1 BP 2 EP 10 DI 10.1097/00005768-199801000-00002 PG 9 WC Sport Sciences SC Sport Sciences GA YU223 UT WOS:000071694600001 PM 9475638 ER PT S AU Ackerman, MJ AF Ackerman, MJ BE Cesnik, B McCray, AT Scherrer, JR TI The Visible Human Project (TM): A resource for anatomical visualization SO MEDINFO '98 - 9TH WORLD CONGRESS ON MEDICAL INFORMATICS, PTS 1 AND 2 SE STUDIES IN HEALTH TECHNOLOGY AND INFORMATICS LA English DT Proceedings Paper CT 9th World Congress on Medical Informatics: Global Health Networking - A Vision for the Next Millennium (MEDINFO 98) CY 1998 CL SEOUL, SOUTH KOREA SP Int Med Informat Assoc DE imaging; anatomy; digital image library AB The National Library of Medicine (NLM) has long been a world leader in the archiving and distribution of the print-based images of biology and medicine. NLM has also been a pioneer in the use of computer systems to encode and distribute textual knowledge of the life sciences. NLM's Long Range Planning effort of 1985-86 foresaw a coming era where NLM's Bibliographic and factual database services would be complemented by libraries of digital images, distributed over high speed computer networks and by high capacity physical media. The NLM Planning Panel on Electronic Imaging recommended that NLM should undertake the building a digital image library consisting of computerized tomography (CT) and magnetic resonance (MR) images, and cryosection images of a representative, carefully selected and prepared male and female cadaver -- the "Visible Human ProjectJ." The male and female Visible Human data sets are now being made available through a license agreement with the NLM. The data sets are supporting a wide range of educational, diagnostic, treatment planning, and commercial uses. The value of this international resource in the public domain increases through its application. Its utility will continue to grow as related databases are attached to it, and as more attributes are given to its image elements. C1 Natl Lib Med, Visible Human Project, Off High Performance Comp & Commun, Bethesda, MD 20894 USA. RP Ackerman, MJ (reprint author), Natl Lib Med, Visible Human Project, Off High Performance Comp & Commun, 8600 Rockville Pike, Bethesda, MD 20894 USA. NR 0 TC 25 Z9 25 U1 0 U2 1 PU I O S PRESS PI AMSTERDAM PA NIEUWE HEMWEG 6B, 1013 BG AMSTERDAM, NETHERLANDS SN 0926-9630 BN 90-5199-407-9 J9 ST HEAL T PY 1998 VL 52 BP 1030 EP 1032 PG 3 WC Medical Informatics SC Medical Informatics GA BM09G UT WOS:000077613500201 PM 10384616 ER PT J AU Eddy, EM O'Brien, DA AF Eddy, EM O'Brien, DA TI Gene expression during mammalian meiosis SO MEIOSIS AND GAMETOGENESIS SE CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY LA English DT Review ID MALE GERM-CELLS; MOUSE SPERMATOGENIC CELLS; FIBROBLAST GROWTH-FACTOR; DNA-POLYMERASE-BETA; TESTIS-SPECIFIC TRANSCRIPTION; RAT SEMINIFEROUS EPITHELIUM; RNA-BINDING PROTEIN; MESSENGER RIBONUCLEIC-ACIDS; IMMUNOELECTRON MICROSCOPIC LOCALIZATION; DEVELOPMENTALLY-REGULATED EXPRESSION C1 NIEHS, Gamete Biol Sect, Reprod & Dev Toxicol Lab, NIH, Res Triangle Pk, NC 27709 USA. Univ N Carolina, Dept Pediat, Reprod Biol Lab, Chapel Hill, NC 27599 USA. Univ N Carolina, Dept Cell Biol & Anat, Chapel Hill, NC 27599 USA. RP Eddy, EM (reprint author), NIEHS, Gamete Biol Sect, Reprod & Dev Toxicol Lab, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. FU NCI NIH HHS [CA 16086]; NICHD NIH HHS [HD26485, P30-HD18968, R01 HD026485] NR 355 TC 124 Z9 126 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0070-2153 J9 CURR TOP DEV BIOL PY 1998 VL 37 BP 141 EP 200 PG 60 WC Developmental Biology SC Developmental Biology GA BK68B UT WOS:000073061700005 PM 9352186 ER PT J AU Cheever, AW Jankovic, D Yap, GS Kullberg, MC Sher, A Wynn, TA AF Cheever, AW Jankovic, D Yap, GS Kullberg, MC Sher, A Wynn, TA TI Role of cytokines in the formation and downregulation of hepatic circumoval granulomas and hepatic fibrosis in Schistosoma mansoni-infected mice SO MEMORIAS DO INSTITUTO OSWALDO CRUZ LA English DT Article; Proceedings Paper CT 6th International Symposium on Schistosomiasis / 6th National Meeting on Schistosomiasis CY OCT 19-24, 1997 CL BELO HORIZONT, BRAZIL SP Fiocruz, Natl Res Council (CNPq), Fapemig, Prefeitura Municipal de Sabara, Prefeitura Municipal de Contagem, Lablaser, Copasa, Centro de Pesquisas Rene Rachou-Fiocruz DE schistosomiasis; granulomas; cytokines; immunoregulation; hepatic fibrosis; type 2 delayed hypersensitivity ID TUMOR-NECROSIS-FACTOR; DIFFERENT CLINICAL FORMS; MURINE SCHISTOSOMIASIS; IFN-GAMMA; RELATIVE CONTRIBUTION; ADOPTIVE SUPPRESSION; COLLAGEN DEPOSITION; IMMUNE-COMPLEXES; CELL RESPONSES; TH2 CYTOKINES AB Schistosoma mansoni infections are associated with a strong Th2 cytokine response. Treatment of mice with IL-12 or anti-IL-2 or anti-IL-4 before i.v. injection of eggs increased IFN-gamma production and downregulated Th2 responses and pulmonary granuloma size. Conversely, anti-IFN-gamma antibody treatment increased Th2 responses and granuloma size. Similar manipulation produced less dramatic results in infected mice. However, sensitization of mice with eggs + IL-12 before infection augmented the Th1 response and decreased Th2 cytokines, granuloma size and fibrosis. Antisera to IFN-gamma, TNF-alpha or IL-12 during IL-12-egg immunization partly restored granuloma size and fibrosis following infection. Variations in the size of granulomas in acute (8 week) infections may be influenced primarily by the number and state of activation of T cells. In chronic (12-16 week) infections immunologic downmodulation proceeded normally in mice without functional CD8+ cells and in IFN-gamma KO mice but not in B cell KO (mu MT) mice or in mice deficient in FcR expression in spite of the fact that these mice downregulated their T cell and cytokine responses. It is evident that the participation of cytokines in granuloma formation and regulation is complicated and that the mechanisms controlling both these phenomena are likely to involve both T cells and antibody/FcR interactions. C1 NIAID, Parasit Dis Lab, Immunobiol Sect, NIH, Bethesda, MD 20892 USA. Inst Biomed Res, Rockville, MD USA. RP Cheever, AW (reprint author), NIAID, Parasit Dis Lab, Immunobiol Sect, NIH, Bethesda, MD 20892 USA. EM achever@atlas.niaid.nih.gov RI Wynn, Thomas/C-2797-2011 NR 66 TC 24 Z9 27 U1 0 U2 2 PU FUNDACO OSWALDO CRUZ PI RIO DE JANEIRO, RJ PA AV BRASIL 4365, 21045-900 RIO DE JANEIRO, RJ, BRAZIL SN 0074-0276 J9 MEM I OSWALDO CRUZ JI Mem. Inst. Oswaldo Cruz PY 1998 VL 93 SU 1 BP 25 EP 32 DI 10.1590/S0074-02761998000700004 PG 8 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA 135HD UT WOS:000076795000004 PM 9921320 ER PT J AU Montenegro, SML Miranda, P Abath, FGC Teixeira, KM Coutinho, EM Domingues, ALC Domingues, L Brinkman, J Goncalves, I Mahanty, S Sher, A Wynn, TA AF Montenegro, SML Miranda, P Abath, FGC Teixeira, KM Coutinho, EM Domingues, ALC Domingues, L Brinkman, J Goncalves, I Mahanty, S Sher, A Wynn, TA TI Preliminary results on the regulatory role of IFN-gamma and IL-10 human Schistosomiasis mansoni SO MEMORIAS DO INSTITUTO OSWALDO CRUZ LA English DT Article; Proceedings Paper CT 6th International Symposium on Schistosomiasis / 6th National Meeting on Schistosomiasis CY OCT 19-24, 1997 CL BELO HORIZONT, BRAZIL SP Fiocruz, Natl Res Council (CNPq), Fapemig, Prefeitura Municipal de Sabara, Prefeitura Municipal de Contagem, Lablaser, Copasa, Centro de Pesquisas Rene Rachou-Fiocruz DE Schistosoma mansoni; spleen cells; IL-10; IFN-gamma C1 FIOCRUZ, Ctr Pesquisas Aggeu Magalhaes, Dept Imunol, BR-50670420 Recife, PE, Brazil. UFPE, LIKA, Recife, PE, Brazil. Ctr Dis Control, Atlanta, GA 30333 USA. NIH, Bethesda, MD 20892 USA. RP Montenegro, SML (reprint author), FIOCRUZ, Ctr Pesquisas Aggeu Magalhaes, Dept Imunol, Av Moraes Rego S-N, BR-50670420 Recife, PE, Brazil. RI Wynn, Thomas/C-2797-2011 NR 0 TC 1 Z9 1 U1 0 U2 0 PU FUNDACO OSWALDO CRUZ PI RIO DE JANEIRO, RJ PA AV BRASIL 4365, 21045-900 RIO DE JANEIRO, RJ, BRAZIL SN 0074-0276 J9 MEM I OSWALDO CRUZ JI Mem. Inst. Oswaldo Cruz PY 1998 VL 93 SU 1 BP 173 EP 173 DI 10.1590/S0074-02761998000700027 PG 1 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA 135HD UT WOS:000076795000027 PM 9921343 ER PT J AU Alexander, D AF Alexander, D TI Prevention of mental retardation: Four decades of research SO MENTAL RETARDATION AND DEVELOPMENTAL DISABILITIES RESEARCH REVIEWS LA English DT Article; Proceedings Paper CT 19th Annual Spectrum of Developmental Disabilities Course CY MAR 18-20, 1996 CL JOHNS HOPKINS MED INST, BALTIMORE, MARYLAND HO JOHNS HOPKINS MED INST DE mental retardation; prevention; screening environment; vaccines; nutrition; genetics; prematurity ID CHILDREN AB In the absence of curative therapy, and with treatment difficult and only partially successful, prevention plays a particularly important role in mental retardation, Research conducted during the past 40 years has identified new causes of mental retardation, new means of early diagnosis, and new ways of prevention, Prenatal diagnosis, newborn screening, dietary supplementation or restriction, hormone replacement, vaccination, and immunotherapy are just some of the techniques that have been applied to prevent mental retardation. Together, these interventions have slightly reduced the overall prevalence of mental retardation, and in some instances have nearly eliminated specific causes, Much remains to be done, including developing better means of early intervention for sociocultural mental retardation and convincing society of the value of investment in such approaches. In addition to these approaches, the research frontiers today are neurobiology, earlier prenatal diagnosis, fetal therapy, gene therapy, and reducing premature birth. The potential of these investigations makes the frontier of prevention research in mental retardation an exciting place to be. (C) 1998 Wiley-Liss, Inc. C1 NICHHD, Bethesda, MD 20892 USA. RP Alexander, D (reprint author), NICHHD, Bldg 31,Room 2A03,MSC 2425,31 Ctr Dr, Bethesda, MD 20892 USA. NR 27 TC 11 Z9 11 U1 1 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 1080-4013 J9 MENT RETARD DEV D R JI Ment. Retard. Dev. Disabil. Res. Rev. PY 1998 VL 4 IS 1 BP 50 EP 58 DI 10.1002/(SICI)1098-2779(1998)4:1<50::AID-MRDD8>3.0.CO;2-0 PG 9 WC Clinical Neurology; Neurosciences; Pediatrics; Psychiatry SC Neurosciences & Neurology; Pediatrics; Psychiatry GA YZ340 UT WOS:000072244600008 ER PT J AU Weinstein, SP Wilson, CM Pritsker, A Cushman, SW AF Weinstein, SP Wilson, CM Pritsker, A Cushman, SW TI Dexamethasone inhibits insulin-stimulated recruitment of GLUT4 to the cell surface in rat skeletal muscle SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article ID FATTY-ACID OXIDATION; GLUCOSE-TRANSPORT; ADIPOSE-CELLS; RESISTANCE; ADIPOCYTES; MEMBRANE AB To test the hypothesis that glucocorticoids reduce insulin-stimulated skeletal muscle glucose transport by inhibiting the recruitment of GLUT4 glucose transporters to the cell surface, we determined the effect of glucocorticoid treatment on cell-surface GLUT4 using the impermeant glucose transporter photolabel, 2-N-4-(1-azi-2,2,2-trifluoroethyl)benzoyl-[2-(3) H]1,3-bis-(D-mannos-4-yloxy)-2-propylamine (ATB-[2-H-3]BMPA), and GLUT4 immunoprecipitation, Male Sprague-Dawley rats were treated with dexamethasone ([Dex] 0.9 mg/kg for 2 days) and compared against pair-fed controls. 2-[H-3]deoxyglucose (2-[H-3]DG) uptake in isolated soleus muscles was measured under conditions in which uptake reflects glucose transport activity. In control muscles, 2-[H-3]DG uptake was stimulated eightfold by insulin (20 nmol/L), Dex treatment reduced maximal insulin-stimulated 2-[H-3]DG uptake by 48% +/- 4% (mean +/- SEM) and decreased cell-surface (ATB-[2-H-3]BMPA-photolabeled) GLUT4 by 48% +/- 3%, despite an increase in total muscle GLUT4 content of 26% +/- 7%, These findings indicate that glucocorticoid-induced inhibition of insulin-stimulated glucose transport in muscle is due to impaired recruitment of GLUT4 to the cell surface. Copyright (C) 1998 by W.B. Saunders Company. C1 NIDDK, Diabet Branch, Expt Diabet Metab & Nutr Sect, NIH, Bethesda, MD 20892 USA. Mt Sinai Sch Med, Dept Med, New York, NY USA. RP Cushman, SW (reprint author), NIDDK, Diabet Branch, Expt Diabet Metab & Nutr Sect, NIH, 10 Ctr Dr,MSC 1420, Bethesda, MD 20892 USA. FU NIDDK NIH HHS [DK-02057] NR 17 TC 61 Z9 64 U1 0 U2 4 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0026-0495 J9 METABOLISM JI Metab.-Clin. Exp. PD JAN PY 1998 VL 47 IS 1 BP 3 EP 6 DI 10.1016/S0026-0495(98)90184-6 PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA ZW524 UT WOS:000074419600002 PM 9440469 ER PT J AU Degerman, E Landstrom, TR Wijkander, J Holst, LS Ahmad, F Belfrage, P Manganiello, V AF Degerman, E Landstrom, TR Wijkander, J Holst, LS Ahmad, F Belfrage, P Manganiello, V TI Phosphorylation and activation of hormone-sensitive adipocyte phosphodiesterase type 3B SO METHODS-A COMPANION TO METHODS IN ENZYMOLOGY LA English DT Article ID CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE; INHIBITED CAMP-PHOSPHODIESTERASE; DEPENDENT PROTEIN-KINASE; RAT ADIPOCYTES; MOLECULAR-CLONING; FAT-CELLS; AMP PHOSPHODIESTERASE; INSULIN; LIPOLYSIS; IDENTIFICATION AB Phosphodiesterases (PDEs) include a large group of structurally related enzymes that belong to at least seven related gene families (PDEs 1-7) that differ in their primary structure, affinity for cAMP and cGMP, response to specific effecters, sensitivity to specific inhibitors, and regulatory mechanism. One characteristic of PDE3s involves their phosphorylation and activation in response to insulin as well as to agents that increase cAMP in adipocytes, hepatocytes, and platelets and in response to insulin-like growth factor 1 in pancreatic beta cells. In adipocytes, activation of the membrane-associated PDE3B is the major mechanism whereby insulin antagonizes catecholamine-induced lipolysis. PDE3B activation results in increased degradation of cAMP and, thereby, a lowering of the activity of cAMP-dependent protein kinase (PKA). The reduced activity of PKA leads to a net dephosphorylation and decreased activity of hormone-sensitive lipase and reduced hydrolysis of triglycerides. Activation of the rat adipocyte PDE3B by insulin is associated with phosphorylation of serine-302. The mechanism whereby insulin stimulation leads to phosphorylation/activation of PDE3B is only partly understood. In rat adipocytes, lipolytic hormones and other agents that increase cAMP, including isoproterenol, also induce rapid phosphorylation, presumably catalyzed by PKA, of serine-302 of PDE3B. The phosphorylation is associated with activation of the enzyme, most likely representing "feedback" regulation of cAMP, presumably allowing close coupling of the regulation of steady-state concentrations of both cAMP and PKA and, thereby, control of lipolysis. In the review we describe methods and strategies used in the authors' laboratories to study phosphorylation and activation of PDE3B in adipocytes and in vitro. (C) 1998 Academic Press. C1 Univ Lund, Dept Cell & Mol Biol, Mol Signalling Lab, Lund, Sweden. NHLBI, Pulm Crit Care Med Branch, NIH, Bethesda, MD 20892 USA. RP Degerman, E (reprint author), Univ Lund, Dept Cell & Mol Biol, Mol Signalling Lab, Lund, Sweden. EM Eva.Degerman@medkem.lu.se NR 58 TC 55 Z9 55 U1 0 U2 3 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1046-2023 J9 METHODS JI Methods PD JAN PY 1998 VL 14 IS 1 BP 43 EP 53 DI 10.1006/meth.1997.0564 PG 11 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ZC565 UT WOS:000072593400005 PM 9500857 ER PT S AU De Groot, AS Jesdale, BM Berzofsky, JA AF De Groot, AS Jesdale, BM Berzofsky, JA BE Kaufmann, SHE Kabelitz, D TI Prediction and determination of MHC ligands and T-cell epitopes SO METHODS IN MICROBIOLOGY, VOL 25: IMMUNOLOGY OF INFECTION SE Methods in Microbiology LA English DT Review ID CLASS-I MOLECULES; PEPTIDE-BINDING MOTIF; HLA-DR ALLELES; ANCHOR RESIDUES; ANTIGENIC PEPTIDES; PRECISE PREDICTION; PROTEIN ANTIGENS; SELF-PEPTIDES; IDENTIFICATION; SEQUENCES C1 Brown Univ, Sch Med, Int Hlth Inst, TB HIV Res Lab, Providence, RI 02912 USA. NCI, Metab Branch, Mol Immunogenet & Vaccine Res Sect, NIH, Bethesda, MD 20892 USA. RP De Groot, AS (reprint author), Brown Univ, Sch Med, Int Hlth Inst, TB HIV Res Lab, Providence, RI 02912 USA. OI De Groot, Annie/0000-0001-5911-1459 NR 81 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0580-9517 BN 0-12-521528-2 J9 METHOD MICROBIOL JI Methods Microbiol. PY 1998 VL 25 BP 79 EP 106 PG 28 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA BL13K UT WOS:000074397200004 ER PT J AU Scidmore, MA Bannantine, J Hackstadt, T AF Scidmore, MA Bannantine, J Hackstadt, T TI Molecular approaches to studying Chlamydia SO METHODS IN MICROBIOLOGY, VOL 27 SE METHODS IN MICROBIOLOGY LA English DT Review ID OUTER-MEMBRANE PROTEIN; POLYMERASE CHAIN-REACTION; HISTONE H1-LIKE PROTEIN; ESCHERICHIA-COLI; RNA-POLYMERASE; RETICULATE BODIES; PSITTACI 6BC; HOST-CELLS; TRACHOMATIS; GENE C1 NIAID, Rocky Mt Labs, Intracellular Parasites Lab, Hamilton, MT 59840 USA. RP Scidmore, MA (reprint author), NIAID, Rocky Mt Labs, Intracellular Parasites Lab, Hamilton, MT 59840 USA. NR 42 TC 0 Z9 0 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0580-9517 J9 METHOD MICROBIOL PY 1998 VL 27 BP 455 EP 464 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA BN14K UT WOS:000080861900038 ER PT J AU Rendell, MS Finnegan, MF Healy, JC Lind, A Milliken, BK Finney, DE Bonner, RF AF Rendell, MS Finnegan, MF Healy, JC Lind, A Milliken, BK Finney, DE Bonner, RF TI The relationship of laser-Doppler skin blood flow measurements to the cutaneous microvascular anatomy SO MICROVASCULAR RESEARCH LA English DT Article AB The hairless plantar paw surface of the rat shows high skin blood flow with a substantial response to thermal stimulation. This contrasts with hair-covered areas such as the back, where there is much lower basal flow and thermal response. These properties are similar to the differences seen in humans between skin sites which have a high density of arterioles and venules (AV areas) and sites with predominantly nutritive (NUTR) capillary perfusion. However, there has been no previous study of the microvascular anatomy of rodent skin. We used NIH Image, a quantitative imaging program, to count the capillaries, arterioles, and venules in the skin of the plantar paw surface and the back of 14 Wistar-Kyoto rats. We also used laser-Doppler techniques to determine skin blood flow at these sites. We found significantly more vessels per unit area at the paw. There were twice as many capillaries in the paw (19.6 +/- 2.4 per mm(2)) compared to the back (9 +/- 1.5 per mm(2)) (P < 0.001). Similarly, there were three times as many venules (11.8 +/- 1.2 per mm(2) vs 3.48 +/- 0.45 per mm(2); P < 0.001). The largest difference was in the number of arterioles (7.76 +/- 0.74 per mm(2) vs 0.79 +/- 0.13 per mm(2) at the back; P < 0.001). The greater microvascular density at the paw was reflected in a threefold higher basal blood flow (6.6 +/- 0.44 ml/min/100 g) compared to that in the back (1.99 +/- 0.07 ml/min/100 g) (P < 0.001). Microvascular volume at the back was 0.14 +/- 0.01 x 10(6) RBC/ml in the basal state compared to 0.31 +/- 0.01 x 10(6) RBC/ml at the paw. Thus, the increased number of vessels at the paw resulted in a twofold increase in microvascular volume. The plantar paw surface has considerably more vessels than the back. As might be expected, there is a higher proportion of arterioles and venules compared to capillaries at the paw than at the back. Thus, the plantar paw surface is an AV site compared to the back, which is a NUTR site. Although our prior studies have largely assumed that we could use the paw and back as contrast sites comparable to AV and NUTR sites in humans, we have now for the first time conclusively established this fact. The increased microvascular density at the paw results in higher skin blood flow at this site. (C) 1998 Academic Press. C1 Creighton Univ, Sch Med, Dept Med, Omaha, NE 68131 USA. Creighton Univ, Sch Med, Dept Pathol, Omaha, NE 68131 USA. NIH, Ctr Res Resources, Biomed Engn & Instrumentat Program, Bethesda, MD 20205 USA. RP Rendell, MS (reprint author), Creighton Diabet Ctr, 601 N 30th St, Omaha, NE 68131 USA. RI Bonner, Robert/C-6783-2015 NR 17 TC 29 Z9 30 U1 2 U2 5 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0026-2862 J9 MICROVASC RES JI Microvasc. Res. PD JAN PY 1998 VL 55 IS 1 BP 3 EP 13 DI 10.1006/mvre.1997.2049 PG 11 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA YY496 UT WOS:000072153300002 PM 9473405 ER PT J AU Allen, JP Cross, GM Fertig, JB Litten, RZ AF Allen, JP Cross, GM Fertig, JB Litten, RZ TI Screening for alcohol problems in the military: Recommended tests SO MILITARY MEDICINE LA English DT Article ID CARBOHYDRATE-DEFICIENT TRANSFERRIN; IDENTIFICATION TEST AUDIT; SERUM TRANSFERRIN; SIALIC-ACID; CHRONIC ETHANOL; CONSUMPTION; SIALYLATION; DRINKING; MARKERS AB This article suggests two new techniques for identifying alcohol problems in a military setting. The Alcohol Use Disorders Identification Test is a well validated self-report procedure and requires approximately 2 minutes for administration, Measurement of carbohydrate-deficient transferrin provides a useful biochemical index of recent heavy alcohol consumption. Employment of these tests could improve selection of individuals seeking entry to the military, aid recognition of current personnel in need of treatment, and assist in evaluating progress of patients in treatment. C1 NIAAA, Bethesda, MD 20892 USA. RP Allen, JP (reprint author), NIAAA, 6000 Execut Blvd,Suite 505, Bethesda, MD 20892 USA. NR 25 TC 7 Z9 7 U1 0 U2 1 PU ASSN MILITARY SURG US PI BETHESDA PA 9320 OLD GEORGETOWN RD, BETHESDA, MD 20814 USA SN 0026-4075 J9 MIL MED JI Milit. Med. PD JAN PY 1998 VL 163 IS 1 BP 9 EP 12 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA YT022 UT WOS:000071556200004 PM 9465564 ER PT J AU Moch, H Bissig, H Richter, J Schaffer, A Desper, R Sauter, G Mihatsch, MJ AF Moch, H Bissig, H Richter, J Schaffer, A Desper, R Sauter, G Mihatsch, MJ TI Genetic heterogeneity underlying development of metastasis in renal cell carcinoma SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Univ Basel, Inst Pathol, CH-4003 Basel, Switzerland. Natl Human Genome Res Inst, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 5A EP 5A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400024 ER PT J AU Sanjuan, X Sohel, ME Yang, J Merino, MJ AF Sanjuan, X Sohel, ME Yang, J Merino, MJ TI Alveolar soft part sarcoma: correlation between genetic alterations, proliferative markers and clinico-pathologic findings SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 15A EP 15A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400083 ER PT J AU Otis, CN Albuquerque, A Sanjuan, X Sobel, M Merino, MJ AF Otis, CN Albuquerque, A Sanjuan, X Sobel, M Merino, MJ TI Loss of heterozygosity in TP53, BRCA1, VHL, and estrogen receptor genes in breast carcinoma: Correlation to related protein products and morphologic features SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Tufts Univ, Sch Med, Baystate Med Ctr, Springfield, MA 01199 USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 24A EP 24A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400140 ER PT J AU Fetsch, PA Marincola, F Filie, A Hijazi, Y Abati, A AF Fetsch, PA Marincola, F Filie, A Hijazi, Y Abati, A TI Melanoma-associated antigen recognized by T-cells (MART-1): The advent of a preferred immunohistochemical antibody for the diagnosis of metastatic malignant melanoma (MMM) in fine needle aspirations (FNA). SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 37A EP 37A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400219 ER PT J AU Filie, A Quezado, M Albuquerque, A Wilson, J Merino, M Abati, A AF Filie, A Quezado, M Albuquerque, A Wilson, J Merino, M Abati, A TI Morphologic diversity in malignant melanoma: The potential use of microdissection (MD) and the polymerase chain reaction (PCR) for diagnosis. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 38A EP 38A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400226 ER PT J AU Sanjuan, X Abati, A Wilder, AM Merino, MJ AF Sanjuan, X Abati, A Wilder, AM Merino, MJ TI Use of microdissection (MD) and polymerase chain reaction (PCR) for the diagnosis of adrenal cortical carcinoma (ACCA) on FNA. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 43A EP 43A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400252 ER PT J AU Simsir, A Fetsch, P Zakowski, M Abati, A AF Simsir, A Fetsch, P Zakowski, M Abati, A TI E-cadherin: A superior marker for the distinction between reactive mesothelial cells and adenocarcinoma in pleural effusions. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 44A EP 44A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400258 ER PT J AU Simsir, A Fetsch, P Mehta, D Zakowski, M Abati, A AF Simsir, A Fetsch, P Mehta, D Zakowski, M Abati, A TI Calretinin: Unfulfilled expectations for the differential diagnosis of malignant pleural effusions. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 44A EP 44A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400257 ER PT J AU Smith, AE Scott, D Faser, C Snell, J Tabbarra, S Thompson, J Dworkin, L Olson, J Sherman, ME Schiffman, MH AF Smith, AE Scott, D Faser, C Snell, J Tabbarra, S Thompson, J Dworkin, L Olson, J Sherman, ME Schiffman, MH TI Review of The Bethesda System atlas does not improve diagnostic reproducibility or accuracy SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Johns Hopkins Med Inst, Baltimore, MD 21205 USA. George Washington Univ, Med Ctr, Washington, DC 20037 USA. Kaiser Permanente, Portland, OR USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 44A EP 44A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400259 ER PT J AU Boni, R Vortmeyer, AO Pack, S Park, WS Burg, G Hofbauer, G Darling, T Liotta, L Zhuang, Z AF Boni, R Vortmeyer, AO Pack, S Park, WS Burg, G Hofbauer, G Darling, T Liotta, L Zhuang, Z TI Somatic mutations of the MEN1 tumor suppressor gene detected in sporadic angiofibromas SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Univ Zurich Hosp, Dept Dermatol, CH-8091 Zurich, Switzerland. NIH, Pathol Lab, Bethesda, MD 20892 USA. NIH, Dept Dermatol, Bethesda, MD 20892 USA. RI Hofbauer, Gunther/B-2671-2010; Pack, Svetlana/C-2020-2014 OI Hofbauer, Gunther/0000-0003-0542-7989; NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 47A EP 47A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400276 ER PT J AU Duray, PH Cole, K Greenberg, P Klein-Szanto, A Emmert-Buck, M Lee, J Liotta, L AF Duray, PH Cole, K Greenberg, P Klein-Szanto, A Emmert-Buck, M Lee, J Liotta, L TI Laser capture microdissection can isolate nevomelanocytes from dysplastic nevi for molecular study SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Fox Chase Canc Ctr, Philadelphia, PA 19111 USA. NCI, Pathol Lab, Bethesda, MD 20892 USA. RI Klein-Szanto, Andres/E-6218-2010; Cole, Kristina/M-3922-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 49A EP 49A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400292 ER PT J AU Kleer, CG Giordano, TJ Merino, MJ AF Kleer, CG Giordano, TJ Merino, MJ TI Squamous cell carcinoma of the thyroid: An aggressive tumor associated with tall cell variant of papillary thyroid carcinoma. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA. NCI, Pathol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 57A EP 57A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400338 ER PT J AU Kleer, CG Bryant, BR Giordano, TJ Sobel, ME Merino, MJ AF Kleer, CG Bryant, BR Giordano, TJ Sobel, ME Merino, MJ TI Genetic changes in chromosome 1p and 17p in thyroid cancer progression. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA. NCI, Pathol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 57A EP 57A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400337 ER PT J AU Lubensky, I Vortmeyer, AO Pack, S Huang, S Debelenko, L Emmert-Buck, M Skarulis, M Spiegel, A Chandrasekharappa, S Collins, F Marx, S Liotta, L Jensen, R Zhuang, Z AF Lubensky, I Vortmeyer, AO Pack, S Huang, S Debelenko, L Emmert-Buck, M Skarulis, M Spiegel, A Chandrasekharappa, S Collins, F Marx, S Liotta, L Jensen, R Zhuang, Z TI Somatic mutations of the MEN1 tumor suppressor gene in sporadic gastrinomas and insulinomas. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NIDDK, Pathol Lab, NCI, NHGRI,NIH, Bethesda, MD USA. RI Pack, Svetlana/C-2020-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 58A EP 58A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400341 ER PT J AU Vortmeyer, AO Pack, SD Weil, R Lubensky, IA Ezzat, SZ Oldfield, EH Asa, SL Zhuang, Z AF Vortmeyer, AO Pack, SD Weil, R Lubensky, IA Ezzat, SZ Oldfield, EH Asa, SL Zhuang, Z TI MEN1 gene alterations detected in MEN1-associated and sporadic pituitary adenomas SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NIH, Pathol Lab, Bethesda, MD 20892 USA. NIH, Surg Neurol Branch, Bethesda, MD 20892 USA. Mt Sinai Hosp, Dept Pathol, Toronto, ON M5G 1X5, Canada. RI Pack, Svetlana/C-2020-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 59A EP 59A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400351 ER PT J AU Fogt, F Vortmeyer, AO Stolte, M Mueller, E Mueller, J Noffsinger, A Zhuang, Z AF Fogt, F Vortmeyer, AO Stolte, M Mueller, E Mueller, J Noffsinger, A Zhuang, Z TI Involvement of the Von Hippel Lindau gene locus in polypoid dysplasia but not flat dysplasia in ulcerative colitis SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Univ Penn, Presbyterian Med Ctr, Dept Pathol, Philadelphia, PA 19104 USA. NCI, Bethesda, MD 20892 USA. Staedt Krankenanstalten, Bayreuth, Germany. Tech Univ Munich, D-8000 Munich, Germany. Univ Cincinnati, Med Ctr, Cincinnati, OH 45267 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 64A EP 64A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400382 ER PT J AU Vortmeyer, AO Lubensky, IA Merino, MJ Wang, C Pham, T Furth, EE Zhuang, Z AF Vortmeyer, AO Lubensky, IA Merino, MJ Wang, C Pham, T Furth, EE Zhuang, Z TI Concordance of genetic changes in colorectal poorly differentiated neuroendocrine carcinomas and associated adenocarcinomas SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NIH, Pathol Lab, Bethesda, MD 20892 USA. Hosp Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 72A EP 72A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400427 ER PT J AU Kamoi, S Park, WS Vortmeyer, A Zhuang, Z Silverberg, SG AF Kamoi, S Park, WS Vortmeyer, A Zhuang, Z Silverberg, SG TI Loss of heterozygosity (LOH) analysis in serous(S) and mucinous(M) benign(B), low malignant potential(L) and carcinomatous(C) ovarian tumors. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 George Washington Univ, Washington, DC 20052 USA. Univ Maryland, Natl Canc Inst, College Pk, MD 20742 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 106A EP 106A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400631 ER PT J AU Otis, CN Quezado, MM Albuquerque, A Bryant, B Sobel, M Merino, MJ AF Otis, CN Quezado, MM Albuquerque, A Bryant, B Sobel, M Merino, MJ TI Correlation of loss of heterozygosity in P53, BRCA1 and estrogen receptor genes in ovarian epithelial tumors of different cell types and biologic behavior SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Tufts Univ, Sch Med, Baystate Med Ctr, Springfield, MA 01199 USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 110A EP 110A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400657 ER PT J AU Quezado, MM Moskaluk, C Bryant, B Mills, S Merino, MJ AF Quezado, MM Moskaluk, C Bryant, B Mills, S Merino, MJ TI Is BRCAI a marker of prognosis in ovarian cancer? SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Univ Virginia, Charlottesville, VA 22903 USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 112A EP 112A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400670 ER PT J AU Quezado, MM Bryant, B Sanjuan, X Otis, CN Merino, MJ AF Quezado, MM Bryant, B Sanjuan, X Otis, CN Merino, MJ TI Histogenesis and genetic changes of ovarian transitional cell carcinomas: Correlation with bladder tumors and prognosis SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Tufts Univ, Sch Med, Baystate Med Ctr, Springfield, MA 01199 USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 112A EP 112A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400669 ER PT J AU Sherman, ME Burks, RT Kurman, RJ Struewing, J Lee, J Hartge, P AF Sherman, ME Burks, RT Kurman, RJ Struewing, J Lee, J Hartge, P TI Findings in benign ovaries at increased risk for developing carcinoma SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, Bethesda, MD 20892 USA. Johns Hopkins Med Inst, Baltimore, MD 21205 USA. Virginia Commonwealth Univ, Med Coll Virginia, Div Hlth Sci, Richmond, VA 23298 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 114A EP 114A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400680 ER PT J AU Werness, BA Freedman, AN Piver, MS AF Werness, BA Freedman, AN Piver, MS TI Poorer prognosis in patients with epithelial ovarian cancer whose tumors express p53, but not p21(wafl/cipl) SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Roswell Pk Canc Inst, Dept Pathol, Buffalo, NY USA. Roswell Pk Canc Inst, Dept Gynecol Oncol, Buffalo, NY 14263 USA. NCI, Genet Epidemiol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 117A EP 117A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400696 ER PT J AU Merino, MJ Alburquerque, A Bryant, B Santamaria, J Esparza, B Merino, F AF Merino, MJ Alburquerque, A Bryant, B Santamaria, J Esparza, B Merino, F TI Genetic alterations in SCC carcinoma of head and neck. Clinico-pathologic correlations. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Univ Basque Country, E-48080 Bilbao, Spain. NCI, Bethesda, MD 20892 USA. RI Santamaria, Joseba Andoni/E-1645-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 121A EP 121A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400724 ER PT J AU Campo, E Miquel, R Krenacs, L Sorbara, L Raffeld, M Jaffe, ES AF Campo, E Miquel, R Krenacs, L Sorbara, L Raffeld, M Jaffe, ES TI Nodal marginal zone lymphomas of malt and "splenic" type SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Univ Barcelona, Hosp Clin, Barcelona, Spain. NCI, Bethesda, MD 20892 USA. RI Krenacs, Laszlo/L-8063-2014 OI Krenacs, Laszlo/0000-0001-6541-3031 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 126A EP 126A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400749 ER PT J AU Chen, ST Medeiros, LJ Jaffe, ES Xu, JF Xiao, S Fletcher, JA Campo, E Stass, SA Abruzzo, LV AF Chen, ST Medeiros, LJ Jaffe, ES Xu, JF Xiao, S Fletcher, JA Campo, E Stass, SA Abruzzo, LV TI Lymphoblastic lymphomas with potential for T-lymphoid and myeloid differentiation are associated with the 1(8;13) fusion transcript. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Natl Canc Inst, Baltimore, MD USA. City Hope Natl Med Ctr, Duarte, CA 91010 USA. Greenebaum Canc Ctr, Baltimore, MD USA. Brigham & Womens Hosp, Boston, MA 02115 USA. Univ Barcelona, Barcelona, Spain. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 126A EP 126A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400752 ER PT J AU Cheng, RZ Guan, XY Lau, GKK Chan, JKC Trent, J Zhuang, ZP Pack, S Raffeld, M Jaffe, ES AF Cheng, RZ Guan, XY Lau, GKK Chan, JKC Trent, J Zhuang, ZP Pack, S Raffeld, M Jaffe, ES TI Chromosome 1p terminal deletion and loss of chromosomes 17p and 16p are common findings in nasal NK/T cell lymphoma by comparative genomic hybridization. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NIH, Pathol Lab, Bethesda, MD 20892 USA. RI Pack, Svetlana/C-2020-2014 NR 0 TC 6 Z9 6 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 126A EP 126A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400753 ER PT J AU Elenitoba-Johnson, KSJ Gascoyne, RD Lim, MS Chhanabai, M Jaffe, ES Raffeld, M AF Elenitoba-Johnson, KSJ Gascoyne, RD Lim, MS Chhanabai, M Jaffe, ES Raffeld, M TI Homozygous deletions at chromosome 9p21 encompassing p16 and p15 are associated with histologic progression in follicle center lymphoma SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 British Columbia Canc Agcy, Dept Pathol, Vancouver, BC V5Z 4E6, Canada. NCI, Hematopathol Sect, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 128A EP 128A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400765 ER PT J AU Greiner, TC Zahm, SH Weisenburger, DD AF Greiner, TC Zahm, SH Weisenburger, DD TI Molecular epidemiology of p53 mutations in non-Hodgkin's lymphomas associated with pesticide exposures SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Univ Nebraska, Med Ctr, Omaha, NE USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 130A EP 130A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400775 ER PT J AU Lim, MS Cheng, RZ Chopra, A Kingma, DW Jaffe, ES Raffeld, M AF Lim, MS Cheng, RZ Chopra, A Kingma, DW Jaffe, ES Raffeld, M TI BCL-6 immunoreactivity demonstrates T-cell rich B-cell non-Hodgkin's lymphoma to be a tumor of germinal center B cells. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NIH, Pathol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 135A EP 135A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400804 ER PT J AU Quintanilla-Martinez, L Thieblemont, C Fend, F Kumar, S Pyniol, M Campo, E Jaffe, ES Raffeld, M AF Quintanilla-Martinez, L Thieblemont, C Fend, F Kumar, S Pyniol, M Campo, E Jaffe, ES Raffeld, M TI Cyclin D1-positive mantle cell lymphomas lack expression of p27(Klp1), a cyclin-dependent kinase inhibitor and a key mediator of the G1/S phase. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Hosp Clin Barcelona, Barcelona, Spain. NCI, Hematopathol Sect, NIH, Bethesda, MD 20892 USA. RI Krenacs, Laszlo/L-8063-2014 OI Krenacs, Laszlo/0000-0001-6541-3031 NR 0 TC 2 Z9 2 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 139A EP 139A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400827 ER PT J AU Quintanilla-Martinez, L Guerrero, I Franklin, JL Naresh, KN Krenacs, L Rama-Rao, C Bhatia, K Magrath, IT Raffeld, M AF Quintanilla-Martinez, L Guerrero, I Franklin, JL Naresh, KN Krenacs, L Rama-Rao, C Bhatia, K Magrath, IT Raffeld, M TI Nasal NK/T-cell lymphoma from Peru. High prevalence of p53 overexpression. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Inst Nacl Enfermedades Neoplast, Lima, Peru. NCI, Hematopathol Sect, NIH, Bethesda, MD 20892 USA. NCI, Pediat Branch, NIH, Bethesda, MD 20892 USA. RI Krenacs, Laszlo/L-8063-2014 OI Krenacs, Laszlo/0000-0001-6541-3031 NR 0 TC 2 Z9 2 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 139A EP 139A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400828 ER PT J AU Hatfill, S Marques, A Margolis, L Duray, PH AF Hatfill, S Marques, A Margolis, L Duray, PH TI Three dimensional tissue explants are substrates for Lyme spirochetes in a NASA-designed bioreactor. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, NICHD, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 145A EP 145A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400865 ER PT J AU Hatfill, S Sylwester, A Margolis, L Lash, A Duray, PH AF Hatfill, S Sylwester, A Margolis, L Lash, A Duray, PH TI In-vitro generation of Kaposi's sarcoma lesions by HHV8 SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 145A EP 145A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400864 ER PT J AU Lash, A Granter, S Masters, E Duray, PH AF Lash, A Granter, S Masters, E Duray, PH TI Erythema migrans (EM) in Missouri compared to EM in the Northeastern US SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, Pathol Lab, Bethesda, MD 20892 USA. Brigham & Womens Hosp, Cape Girardeau, MO USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 145A EP 145A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400867 ER PT J AU Duray, PH Krizman, D Vocke, E Chuaqui, RF Lyne, C Linehan, WM Cole, K Pappalardo, PA Liotta, LA Emmert-Buck, M AF Duray, PH Krizman, D Vocke, E Chuaqui, RF Lyne, C Linehan, WM Cole, K Pappalardo, PA Liotta, LA Emmert-Buck, M TI Construction of a prostate carcinoma cDNA library from a single patient. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, Lab Pathol & Surg Oncol, Bethesda, MD 20892 USA. RI Cole, Kristina/M-3922-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 163A EP 163A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793400973 ER PT J AU Fidelia-Lamberg, M Kotoula, V Tsokos, M AF Fidelia-Lamberg, M Kotoula, V Tsokos, M TI Loss of heterozygosity at the human tyrosine hydroxylase (TH) locus in solid pediatric tumors. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 168A EP 168A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401001 ER PT J AU Kumar, S Pack, S Walker, R Kumar, D Zhuang, Z Meltzer, P Tsokos, M AF Kumar, S Pack, S Walker, R Kumar, D Zhuang, Z Meltzer, P Tsokos, M TI Detection of EWS-FLI-1 fusion in Ewing's sarcoma primitive neuroectodermal tumor (ES/PNET) by fluorescence in-situ hybridization (FISH) using formalin fixed paraffin embedded (FFPE) tissue. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, NHGRI, Pathol Lab, NIH, Bethesda, MD 20892 USA. Univ Maryland, Baltimore, MD 21201 USA. RI Pack, Svetlana/C-2020-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 168A EP 168A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401005 ER PT J AU Mitsiades, N Poulaki, V Kotoula, V Leone, A Tsokos, M AF Mitsiades, N Poulaki, V Kotoula, V Leone, A Tsokos, M TI Tumors of the Ewing's sarcoma family (ESF) express functional Fas ligand (FASL). SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NIH, Pathol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 169A EP 169A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401007 ER PT J AU Brambilla, E Debelenko, L Brambilla, C Agarwal, SK Swalwell, JL Kester, MB Lubensky, IA Zhuang, Z Guru, SC Manickman, P Olufemi, SE Chandrasekharappa, SC Crabtree, JS Kim, YS Heppner, C Burns, AL Spiegel, AM Marx, SJ Liotta, LA Collins, FS Emmert-Buck, M Travis, WD AF Brambilla, E Debelenko, L Brambilla, C Agarwal, SK Swalwell, JL Kester, MB Lubensky, IA Zhuang, Z Guru, SC Manickman, P Olufemi, SE Chandrasekharappa, SC Crabtree, JS Kim, YS Heppner, C Burns, AL Spiegel, AM Marx, SJ Liotta, LA Collins, FS Emmert-Buck, M Travis, WD TI Identification of MEN1 gene mutations in sporadic carcinoid tumors of the lung SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Lung Canc Res Grp, Grenoble, France. CHU Grenoble, F-38043 Grenoble, France. NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. NIDDK, Metab Dis Branch, NIH, Bethesda, MD USA. NIH, Natl Human Genome Res Inst, Lab Gene Transfer, Bethesda, MD USA. AFIP, Dept Pulm Pathol, Washington, DC USA. RI Brambilla, Elisabeth/L-8796-2013; gazzeri, sylvie/M-1961-2013; BRAMBILLA, CHRISTIAN/K-2285-2013 NR 0 TC 0 Z9 0 U1 0 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 171A EP 171A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401022 ER PT J AU Fleming, M Caporaso, N Ross, W Bennett, W Travis, W Midthun, D Jett, J Tazelaar, H Trastek, V Pairolero, P Liotta, L Harris, C AF Fleming, M Caporaso, N Ross, W Bennett, W Travis, W Midthun, D Jett, J Tazelaar, H Trastek, V Pairolero, P Liotta, L Harris, C TI Ki.67 staining (Ki.67%), a predictor of survival and performance status, is associated with apoptosis, necrosis, grade, histology, and smoking in non-small cell lung carcinomas (NSCC) SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, Human Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. NCI, Genet Epidemiol Branch, NIH, Bethesda, MD 20892 USA. Mayo Clin, Rochester, MN USA. AFIP, Dept Pulm & Mediastinal Pathol, Washington, DC USA. AFIP, Sci Labs, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 173A EP 173A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401034 ER PT J AU Flieder, DB Brambilla, E Rush, W Przygodzki, R Fleming, M Freedman, A Travis, W Caporaso, N Guinee, D Bennett, W Jones, R Trastek, V Pairolero, P Tazelaar, H Jett, J Midthun, D Liotta, L Harris, CC AF Flieder, DB Brambilla, E Rush, W Przygodzki, R Fleming, M Freedman, A Travis, W Caporaso, N Guinee, D Bennett, W Jones, R Trastek, V Pairolero, P Tazelaar, H Jett, J Midthun, D Liotta, L Harris, CC TI Rb and p16 expression in non-small cell lung cancer (NSCLC). SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, Human Carcinogenesis Lab, Bethesda, MD 20892 USA. NCI, Genet Epidemiol Branch, Bethesda, MD 20892 USA. CHRU, Pathol Cellulaire Lab, Grenoble, France. AFIP, Washington, DC USA. Univ Utah, Dept Pathol, Salt Lake City, UT 84112 USA. Mayo Clin, Rochester, MN USA. RI Brambilla, Elisabeth/L-8796-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 173A EP 173A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401036 ER PT J AU Galateau-Salle, F Luna, RE Horiba, K Sheppard, MN Hayashi, T Fleming, MV Colby, TV Bennett, W Harris, CC Stetler-Stevenson, WG Liotta, LA Ferrans, VJ Travis, WD AF Galateau-Salle, F Luna, RE Horiba, K Sheppard, MN Hayashi, T Fleming, MV Colby, TV Bennett, W Harris, CC Stetler-Stevenson, WG Liotta, LA Ferrans, VJ Travis, WD TI Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in bronchial squamous preneoplastic lesions (BSPL). SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 CHU Caen, F-14000 Caen, France. NHLBI, NIH, Bethesda, MD 20892 USA. NCI, NIH, Bethesda, MD 20892 USA. Royal Brompton Hosp, London SW3 6LY, England. Nagasaki Univ Hosp, Nagasaki, Japan. Mayo Clin, Scottsdale, AZ USA. AFIP, Washington, DC USA. RI Stetler-Stevenson, William/H-6956-2012; Galateau Salle, Francoise/J-1530-2015 OI Stetler-Stevenson, William/0000-0002-5500-5808; Galateau Salle, Francoise/0000-0003-3642-0165 NR 0 TC 2 Z9 2 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 174A EP 174A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401040 ER PT J AU Jung, SH Lloyd, RV Rush, WR Przygodzki, R Caporaso, N Fleming, MV Bennett, W Pairolero, P Trastek, V Jett, JR Liotta, LA Harris, CC Tazelaar, HD Travis, WD AF Jung, SH Lloyd, RV Rush, WR Przygodzki, R Caporaso, N Fleming, MV Bennett, W Pairolero, P Trastek, V Jett, JR Liotta, LA Harris, CC Tazelaar, HD Travis, WD TI In-situ hybridization (ISH) for transforming growth factor (TGF) beta 1, beta 3, and TGF-receptor II (RII) mRNA in non-small cell lung carcinoma (NSCLC). SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Yonsei Univ, Wonju, South Korea. Mayo Clin, Rochester, MN USA. Mayo Clin, Scottsdale, AZ USA. AFIP, Washington, DC USA. NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 176A EP 176A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401050 ER PT J AU Linnoila, RI Bunnag, T Jensen-Taubman, S Goher, A Steinberg, S Witchi, HP AF Linnoila, RI Bunnag, T Jensen-Taubman, S Goher, A Steinberg, S Witchi, HP TI Alterations in epithelial differentiation patterns during experimental lung carcinogenesis SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, NIH, Rockville, MD USA. Univ Calif Davis, Inst Toxicol & Environm Hlth, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 177A EP 177A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401060 ER PT J AU Rush, W Caporaso, N Freedman, A Bennett, W Fleming, M Midthun, D Tazelaar, H Pairolero, P Trastek, V Liotta, L Harris, C Travis, W AF Rush, W Caporaso, N Freedman, A Bennett, W Fleming, M Midthun, D Tazelaar, H Pairolero, P Trastek, V Liotta, L Harris, C Travis, W TI Transforming growth factor beta receptor type II expression in non-small cell lung carcinomas. SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Armed Forces Inst Pathol, Washington, DC 20306 USA. NCI, Human Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. NCI, Genet Epidemiol Branch, NIH, Bethesda, MD 20892 USA. NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. Mayo Clin, Rochester, MI USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 179A EP 179A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401070 ER PT J AU Sheppard, MN Luna, R Horiba, K Galateau-Salle, F Gal, A Franklin, W Miller, Y Fleming, MV Liotta, LA Statler-Stevenson, WG Ferrans, VJ Travis, WD AF Sheppard, MN Luna, R Horiba, K Galateau-Salle, F Gal, A Franklin, W Miller, Y Fleming, MV Liotta, LA Statler-Stevenson, WG Ferrans, VJ Travis, WD TI Extracellular matrix (ECM) remodelling in neuroendocrine (NEC) proliferations of th lung: Idiopathic diffuse hyperplasia of pulmonary neuroendocrine cells (IDHPNEC), tumorlets (TL), and carcinoid tumors (CT). SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 Royal Brompton Hosp, London SW3 6LY, England. CHU Caen, F-14000 Caen, France. Emory Univ, Atlanta, GA 30322 USA. Univ Colorado, Denver, CO 80202 USA. NHLBI, NIH, Bethesda, MD 20892 USA. NCI, NIH, Bethesda, MD 20892 USA. Armed Forces Inst Pathol, Washington, DC 20306 USA. RI Galateau Salle, Francoise/J-1530-2015 OI Galateau Salle, Francoise/0000-0003-3642-0165 NR 0 TC 1 Z9 1 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 179A EP 179A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401072 ER PT J AU Teruya-Feldstein, J Berkowitz, JR Bud, PR Kingma, DW Mueller, BU Tosato, G Jaffe, ES AF Teruya-Feldstein, J Berkowitz, JR Bud, PR Kingma, DW Mueller, BU Tosato, G Jaffe, ES TI Chemokine mRNA expression in lymphocytic interstitial pneumonitis tissues from HIV-infected pediatric patients SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, Bethesda, MD 20892 USA. US FDA, Bethesda, MD 20014 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 180A EP 180A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401075 ER PT J AU Fend, F Emmert-Buck, MR Lee, J Cole, K Chaqui, RF Liotta, LA Raffeld, M AF Fend, F Emmert-Buck, MR Lee, J Cole, K Chaqui, RF Liotta, LA Raffeld, M TI Immuno-LCM: laser capture microdissection of immunostained frozen sections for RNA analysis SO MODERN PATHOLOGY LA English DT Meeting Abstract C1 NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. RI Cole, Kristina/M-3922-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1998 VL 11 IS 1 BP 185A EP 185A PG 1 WC Pathology SC Pathology GA YV140 UT WOS:000071793401106 ER PT S AU Vogel, FR AF Vogel, FR BE Brown, F Haaheim, LR TI Adjuvants in perspective SO MODULATION OF THE IMMUNE RESPONSE TO VACCINE ANTIGENS SE DEVELOPMENTS IN BIOLOGICAL STANDARDIZATION LA English DT Article; Proceedings Paper CT Symposium on Modulation of the Immune Response to Vaccine Antigens CY JUN 18-21, 1996 CL UNIV BERGEN, BERGEN, NORWAY SP Univ Bergen, Int Assoc Biol Stand, IABS Task Force Vaccines, WHO, European Commiss COST/STD Initiat HO UNIV BERGEN DE immunological adjuvants; vaccine ID ADP-RIBOSYLTRANSFERASE ACTIVITY; IMMUNOGLOBULIN-A RESPONSES; CHOLERA-TOXIN; EXOGENOUS ANTIGENS; ORAL IMMUNIZATION; ANTIBODY-RESPONSE; INFLUENZA VACCINE; PERTUSSIS TOXIN; CELL SUBSETS; TH2 CELLS AB Many vaccines currently under development and testing are composed of synthetic, recombinant, or highly purified subunit antigens. Vaccines composed of these subunit antigens are often considered to be safer than whole-inactivated or live-attenuated vaccines. However, vaccines containing purified subunit antigens are often less immunogenic than traditional vaccines. Immunological adjuvants are agents that enhance specific immune responses to vaccines. Formulation of vaccines with potent adjuvants is an attractive approach for enhancing immune responses to subunit antigens. Adjuvants have diverse mechanisms of action and should be selected for use based on the route of administration and the type of immune response (antibody, cell-mediated, or mucosal) desired for a particular vaccine. Adjuvant mechanisms of action include: (i) increasing the biological or immunological half-life of vaccine antigens; (ii) improving antigen delivery and presentation; and (iii) inducing the production of immunomodulatory cytokines. Through modulation of cytokine responses, adjuvant formulations can be designed that favour the development of Th1 (type 1) or Th2 (type 2) immune responses to vaccine antigens. Novel adjuvants are currently undergoing preclinical and clinical testing with experimental vaccines, including vaccines against HIV-1. Standardized preclinical adjuvant safety tests are also being developed. C1 NIAID, Div Aids, Vaccine & Prevent Res Program, Bethesda, MD 20892 USA. RP Vogel, FR (reprint author), NIAID, Div Aids, Vaccine & Prevent Res Program, MSC-7620,6003 Execut Blvd,Room 2A28A, Bethesda, MD 20892 USA. NR 49 TC 42 Z9 45 U1 0 U2 1 PU KARGER PI BASEL PA POSTFACH, CH-4009 BASEL, SWITZERLAND SN 0301-5149 BN 3-8055-6640-9 J9 DEV BIOL STAND JI Dev.Biol.Stand. PY 1998 VL 92 BP 241 EP 248 PG 8 WC Biology; Immunology; Infectious Diseases; Medicine, Research & Experimental SC Life Sciences & Biomedicine - Other Topics; Immunology; Infectious Diseases; Research & Experimental Medicine GA BK54S UT WOS:000072547000035 PM 9554280 ER PT J AU Gao, B Jaffe, H Kunos, G AF Gao, B Jaffe, H Kunos, G TI Histone H1 isoforms purified from rat liver bind nonspecifically to the nuclear factor 1 recognition sequence and serve as generalized transcriptional repressors SO MOLECULAR AND CELLULAR BIOCHEMISTRY LA English DT Article DE histone H1; nuclear factor 1; non-specific DNA binding; transcriptional repressor ID ADRENERGIC-RECEPTOR GENE; RNA POLYMERASE-II; JUNCTION DNA; IN-VIVO; H-1; PROMOTER; VARIANT; PROTEIN; EXPRESSION; MOUSE AB Two polypeptides with molecular masses of 34 and 30 kDa were copurified from rat liver during DNA affinity purification of a sequence-specific transcription factor binding to the footprint II sequence within the P2 promoter of the rat alpha(1B) adrenergic receptor (alpha(1B)AR) gene, and were identified by microsequencing their endoproteinase Lys-C-derived peptides as histone H1d and histone H1c, respectively. Histone H1 was previously reported to bind to the nuclear factor 1 (NF1) recognition sequence, although the specificity of this binding has been controversial. Here, DNA mobility shift and supershift assays, DNase I footprinting and mutational analyses indicated that the binding of histone H1 to the NF1 sites located within footprint II of the alpha(1B)AR gene P2 promoter is nonspecific. Transient cotransfections into Hep3B cells of histone H1d cDNA with CAT constructs containing promoter regions of different genes resulted in generalized and non-specific suppression of CAT activity. The histone H1d-mediated repression of the activities of the alpha(1B)AR gene P-2/CAT or beta(2)AR gene P(-186/1307)/CAT constructs was reversed by the cotransfection of a cDNA encoding the sequence-specific transcription factor NF1/X, and the fold increase in CAT activities was similar to that obtained in the absence of histone H1d. These results suggest that sequence-specific transcription factors counteract the histone H1-mediated transcriptional repression in vivo by a true activation, which is different from the in vitro antirepression in histone H1-repressed chromatin templates (Laybourn and Kadonaga, (1991) Science 254: 238-245). C1 Virginia Commonwealth Univ, Med Coll Virginia, Dept Pharmacol & Toxicol, Richmond, VA 23298 USA. NINDS, NIH, Bethesda, MD 20892 USA. RP Gao, B (reprint author), Virginia Commonwealth Univ, Med Coll Virginia, Dept Pharmacol & Toxicol, Box 980613, Richmond, VA 23298 USA. NR 40 TC 9 Z9 10 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0300-8177 J9 MOL CELL BIOCHEM JI Mol. Cell. Biochem. PD JAN PY 1998 VL 178 IS 1-2 BP 187 EP 196 DI 10.1023/A:1006843514666 PG 10 WC Cell Biology SC Cell Biology GA ZC647 UT WOS:000072602500025 PM 9546599 ER PT J AU Swirnoff, AH Apel, ED Svaren, J Sevetson, BR Zimonjic, DB Popescu, NC Milbrandt, J AF Swirnoff, AH Apel, ED Svaren, J Sevetson, BR Zimonjic, DB Popescu, NC Milbrandt, J TI Nab1, a corepressor of NCFI-A (Egr-1), contains an active transcriptional repression domain SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID NUCLEAR-LOCALIZATION SIGNAL; II GENE-TRANSCRIPTION; CELL-CYCLE CONTROL; PROTEIN NGFI-A; ACTIVATION DOMAIN; BINDING PROTEIN; RETINOBLASTOMA PROTEIN; MAMMALIAN-CELLS; LUNG-CARCINOMA; GROWTH-FACTORS AB Nab proteins constitute an evolutionarily conserved family of corepressors that specifically interact with and repress transcription mediated by three members of the NGFI-A (Egr-1, Krox24, zif/268) family of immediate-early gene transcription factors, which includes NGFI-C, Krox20, and Egr3, We explored the mechanism of Nab1 repression and identified structural domains required for Nab1 function. Nab1 does not act by blocking DNA binding or nuclear localization of NGFI-A. In fact, Nab1 repression is not unique to NGFI-A because multiple types of non-NGFI-A activation domains were repressed, as was a heterologous transcription factor carrying the NGFI-A RI domain, which is required for Nab1 interaction. Additionally, Nab1 tethered directly to DNA repressed constitutively active promoters. Tethered repression was not dependent on the identity of the basal promoter elements, the presence of a distal enhancer, or the distance separating the binding sites from the promoter. These results suggest that Nab1 repression is not specific to particular activators and that Nab1 is an active repressor that works by a direct mechanism. We identified a bipartite-like nuclear localization sequence and localized the repression function to the Nab conserved domain 2 (NCD2), a region found in the carboxy-terminal half of all Nab proteins, Three small regions of homology between Nab1 and previously characterized corepressors, Drl and E1b 55-kDa protein, were identified within NCD2, Replacement mutagenesis of residues conserved between these proteins interfered with Nab1 repression, although Nab1 does not function by the same mechanism as Dr1. The human NAB1 genomic locus was mapped to chromosome 2q32.3-33. C1 Washington Univ, Sch Med, Dept Pathol, Div Lab Med, St Louis, MO 63110 USA. Washington Univ, Sch Med, Dept Internal Med, Div Lab Med, St Louis, MO 63110 USA. Washington Univ, Sch Med, Dept Mol Biol & Pharmacol, St Louis, MO 63110 USA. NCI, Expt Carcinogenesis Lab, Bethesda, MD 20892 USA. RP Milbrandt, J (reprint author), Washington Univ, Sch Med, Dept Pathol, Div Lab Med, Box 8118,660 S Euclid Ave, St Louis, MO 63110 USA. FU NCI NIH HHS [5 P01 CA49712-08]; NIGMS NIH HHS [1F32GM18058-01, F32 GM018058] NR 87 TC 77 Z9 80 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD JAN PY 1998 VL 18 IS 1 BP 512 EP 524 PG 13 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA YN687 UT WOS:000071195700052 PM 9418898 ER PT S AU Alkon, DL AF Alkon, DL BE Ehrlich, YH TI Molecular specificity of synaptic changes responsible for associative memory SO MOLECULAR AND CELLULAR MECHANISMS OF NEURONAL PLASTICITY: BASIC AND CLINICAL IMPLICATIONS SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT Symposium on Molecular and Cellular Mechanisms of Neuronal Plasticity CY DEC 04, 1996 CL STATEN ISL, NEW YORK ID PROTEIN-KINASE-C; HIPPOCAMPAL-NEURONS; K+-CHANNELS; IONIC CURRENTS; CALCIUM; PHOTORECEPTOR; STORAGE; CELLS; FOLD; GTP C1 NINDS, Lab Adapt Syst, NIH, Bethesda, MD 20892 USA. RP Alkon, DL (reprint author), NINDS, Lab Adapt Syst, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA. NR 48 TC 3 Z9 3 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46040-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 446 BP 1 EP 15 PG 15 WC Behavioral Sciences; Medicine, Research & Experimental; Neurosciences SC Behavioral Sciences; Research & Experimental Medicine; Neurosciences & Neurology GA BM98B UT WOS:000080334400001 PM 10079834 ER PT S AU Tomsic, D Romano, A Maldonado, H AF Tomsic, D Romano, A Maldonado, H BE Ehrlich, YH TI Behavioral and mechanistic bases of long-term habituation in the crab Chasmagnathus SO MOLECULAR AND CELLULAR MECHANISMS OF NEURONAL PLASTICITY: BASIC AND CLINICAL IMPLICATIONS SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT Symposium on Molecular and Cellular Mechanisms of Neuronal Plasticity CY DEC 04, 1996 CL STATEN ISL, NEW YORK ID DEPENDENT PROTEIN-KINASE; ANGIOTENSIN-II; DANGER STIMULUS; CONTEXT MEMORY; GRANULATUS; MODULATION; INHIBITION; AMNESIA; POTENT; SYSTEM C1 Univ Buenos Aires, Fac Ciencias Exactas & Nat, Lab Fisiol Comportamiento Anim, Dept Biol, Buenos Aires, DF, Argentina. RP Tomsic, D (reprint author), NINDS, Lab Adapt Syst, NIH, Bldg 36,Rm B308, Bethesda, MD 20892 USA. NR 55 TC 14 Z9 14 U1 0 U2 1 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-46040-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1998 VL 446 BP 17 EP 35 PG 19 WC Behavioral Sciences; Medicine, Research & Experimental; Neurosciences SC Behavioral Sciences; Research & Experimental Medicine; Neurosciences & Neurology GA BM98B UT WOS:000080334400002 PM 10079835 ER PT J AU Tumanov, AV Nedospasov, SA Turetskaya, RL AF Tumanov, AV Nedospasov, SA Turetskaya, RL TI Chromatin organization in the tumor necrosis factor/lymphotoxin gene locus: Correlation with tissue-specific expression SO MOLECULAR BIOLOGY LA English DT Article DE tumor necrosis factor; lymphotoxin; chromatin; gene expression ID LYMPHOTOXIN TNF-BETA; FACTOR-ALPHA GENE; TRANSCRIPTIONAL REGULATION; DNASE-I; B-CELL; ACTIVATION; NUCLEASE; MOUSE AB A correlation was found between the organization of tumor necrosis factor/lymphotoxin genes in chromatin and their expression. Mapping of the DNase I hypersensitive sites revealed differences in chromatin structure between cells differing in the intensity of TNF/LT gene expression. C1 Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 117984, Russia. NCI, Frederick, MD 21702 USA. SAIC, Frederick, MD 21702 USA. RP Tumanov, AV (reprint author), Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 117984, Russia. RI Nedospasov, Sergei/J-5936-2013; Nedospasov, Sergei/L-1990-2015; Nedospasov, Sergei/Q-7319-2016 NR 24 TC 1 Z9 1 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA CONSULTANTS BUREAU, 233 SPRING ST, NEW YORK, NY 10013 USA SN 0026-8933 J9 MOL BIOL+ JI Mol. Biol. PD JAN-FEB PY 1998 VL 32 IS 1 BP 83 EP 87 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ZC166 UT WOS:000072547400010 ER PT J AU Morley, BJ Li, HS Hiel, H Drescher, DG Elgoyhen, AB AF Morley, BJ Li, HS Hiel, H Drescher, DG Elgoyhen, AB TI Identification of the subunits of the nicotinic cholinergic receptors in the rat cochlea using RT-PCR and in situ hybridization SO MOLECULAR BRAIN RESEARCH LA English DT Article DE mRNA; gene expression; cochlea; hair cells; spiral ganglion; nicotinic acetylcholine receptor subunits; in situ hybridization; RT-PCR ID OUTER HAIR-CELLS; ACETYLCHOLINE-RECEPTOR; GENE FAMILY; GUINEA-PIG; INNERVATION; EXPRESSION; MECHANISM; ALPHA-5; SYSTEM; INNER AB There are two tissues in the adult mammalian cochlea that are post-synaptic to cholinergic efferent fibers: The outer hair cells (OHCs) and the dendrites of the afferent fibers of the type I spiral ganglion cells. The unusual nicotinic-like pharmacology of cochlear cholinergic responses and the unique embryonic development of cochlear tissues suggest that the inner-ear nicotinic cholinergic receptor (nAChR) may be different from nAChRs described previously at synapses in the mammalian brain, autonomic ganglia, or skeletal muscle. In this study, we determined the mRNA expression of the alpha 2-7, alpha 9, and beta 2-4 subunits of the nicotinic acetylcholine receptor (nAChR) family in the rat cochlea. In micro-dissected tissue from the organ of Corti, spiral ganglion, and the membranous lateral wall, we found mRNA expression of the alpha 7 and alpha 9 subunits in the organ of Corti and alpha 5-7, and beta 2 and beta 3 in the spiral ganglion using RT-PCR. Employing in situ hybridization with S-35-riboprobes, we localized alpha 9 in hair cells regions and alpha 6, alpha 7 and beta 2 in the type I cells of the spiral ganglion. No evidence of nAChR subunit mRNA expression was found in supporting cells, but beta 2 was expressed in type II spiral ganglion cells, which are neither cholinergic nor cholinoceptive. (C) 1998 Elsevier Science B.V. C1 Boys Town Natl Res Hosp, Neurochem Lab, Omaha, NE 68131 USA. NIDCD, NIH, Neurochem Lab, Bethesda, MD USA. Wayne State Univ, Dept Otolaryngol & Biochem, Lab Biootol, Detroit, MI USA. CONICET, Inst Invest Farmacol, RA-1033 Buenos Aires, DF, Argentina. RP Morley, BJ (reprint author), Boys Town Natl Res Hosp, Neurochem Lab, 555 N 30th St, Omaha, NE 68131 USA. EM morley@boystown.org NR 44 TC 50 Z9 50 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD JAN PY 1998 VL 53 IS 1-2 BP 78 EP 87 DI 10.1016/S0169-328X(97)00272-6 PG 10 WC Neurosciences SC Neurosciences & Neurology GA YZ686 UT WOS:000072280400008 ER PT J AU Messersmith, DJ Kim, DJ Iadarola, MJ AF Messersmith, DJ Kim, DJ Iadarola, MJ TI Transcription factor regulation of prodynorphin gene expression following rat hindpaw inflammation SO MOLECULAR BRAIN RESEARCH LA English DT Article DE cyclic AMP response element binding protein; CREB phosphorylation; c-Jun; Fos; Fra; dynorphin; nociception ID DEPENDENT PROTEIN-KINASE; SPINAL-CORD NEURONS; ELEMENT-BINDING PROTEIN; C-FOS TRANSCRIPTION; PERIPHERAL INFLAMMATION; MOLECULAR MECHANISMS; CREB PHOSPHORYLATION; JUN; ACTIVATION; DYNORPHIN AB Both c-Fos and prodynorphin mRNA and peptide increase unilaterally in nociceptive-specific neurons in the lumbar rat spinal cord during chronic hindpaw inflammation. To study the mechanisms underlying prodynorphin gene expression, we examined transcription factors and their interactions at the CRE/AP-1-like site, DYNCRE3, found in the prodynorphin gene promoter. CREB repressed while c-Fos and c-Jun activated transcription through the DYNCRE3 site in transient co-transfections in PC12 cells. Following inflammation of the rat hindpaw, immunostaining demonstrated a bilateral increase in phosphorylated CREB (P-CREB)-positive neurons in the spinal cord. Gel supershift studies showed that spinal cord extracts contained CREB, P-CREB, and phosphorylated c-Jun (P-c-Jun) proteins that bound to the DYNCRE3 site. We propose a model in which inflammation-induced phosphorylation of CREB relieves CREB repression at the DYNCRE3 site, P-CREB binds to the c-Fos promoter, and Fos/Fra, P-CREB, and P-c-Jun interact at the DYNCRE3 site to activate prodynorphin gene transcription. (C) 1998 Elsevier Science B.V. C1 NIDR, Pain & Neurosensory Mechanisms Branch, NIH, Bethesda, MD 20892 USA. RP Messersmith, DJ (reprint author), Uniformed Serv Univ Hlth Sci, Dept Anat & Cell Biol, 4301 Jones Bridge Rd, Bethesda, MD 20814 USA. NR 46 TC 19 Z9 19 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD JAN PY 1998 VL 53 IS 1-2 BP 259 EP 269 DI 10.1016/S0169-328X(97)00308-2 PG 11 WC Neurosciences SC Neurosciences & Neurology GA YZ686 UT WOS:000072280400026 ER PT J AU Marietta, C Palombo, F Gallinari, P Jiricny, J Brooks, PJ AF Marietta, C Palombo, F Gallinari, P Jiricny, J Brooks, PJ TI Expression of long-patch and short-patch DNA mismatch repair proteins in the embryonic and adult mammalian brain SO MOLECULAR BRAIN RESEARCH LA English DT Article DE cerebellum; DNA repair; cell nucleus; deamination; DNA replication; brain development ID NONPOLYPOSIS COLON-CANCER; POLYMERASE-BETA; MUTATIONS; HOMOLOG; CELLS; ASSOCIATION; REPLICATION; EXTRACTS; GENES; GTBP AB Expression of the DNA mismatch repair (MMR) pathway was examined in the adult and developing rat brain. Rat homologues of human GTBP and MSH2, which are essential components of the post-replicative DNA MMR system, were identified in nuclear extracts from the adult and developing rat brain. Developmental studies showed that both GTBP and MSH2 levels were higher in nuclei isolated from the embryonic brain (day 16) than adult brain. However, this difference was not as dramatic as the difference in the number of proliferating cells. Levels of thymine DNA glycosylase (TDG), the enzyme which catalyzes the first step in short patch G:T mismatch repair, were also decreased in adult compared to embryonic brain. In the adult brain, MMR proteins were elevated in nuclear extracts enriched for neuronal nuclei. These results suggest that adult brain cells have the capacity to carry out DNA mismatch repair, in spite of a lack of ongoing DNA replication. (C) 1998 Elsevier Science B.V. C1 NIAAA, Mol Neurobiol Sect, Neurogenet Lab, NIH,DICBR,LING, Bethesda, MD 20892 USA. Ist Ric Biol Mol P Angeletti, I-00040 Pomezia, Italy. Inst Med Radiobiol, CH-8029 Zurich, Switzerland. RP Brooks, PJ (reprint author), NIAAA, Mol Neurobiol Sect, Neurogenet Lab, NIH,DICBR,LING, 12420 Parklawn Dr,Room 451 MSC 8110, Bethesda, MD 20892 USA. NR 28 TC 22 Z9 23 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD JAN PY 1998 VL 53 IS 1-2 BP 317 EP 320 DI 10.1016/S0169-328X(97)00311-2 PG 4 WC Neurosciences SC Neurosciences & Neurology GA YZ686 UT WOS:000072280400035 ER PT J AU Wang, XB Uhl, GR AF Wang, XB Uhl, GR TI Subtracted differential display: Genes with amphetamine-altered expression patterns include calcineurin SO MOLECULAR BRAIN RESEARCH LA English DT Article DE PCR differential display; subtraction; gene regulation; amphetamine; drug abuse ID CALMODULIN-BINDING PROTEIN; C-FOS; OPIATE WITHDRAWAL; CHRONIC COCAINE; BRAIN; PHOSPHATASE; STRIATUM AB Genes whose expression changes with administration of abused substances provide candidate biochemical mechanisms for drug-induced long-term brain changes. To identify such genes, and to avoid the false-positive results frequently obtained from differential display PCR, we applied a subtracted differential display (SDD) approach. We subtracted single-stranded cDNA prepared from drug-treated animals with excess mRNA from saline-treated animals, and visa versa, prior to differential display amplifications. Two of the initial amphetamine-regulated cDNAs identified in this fashion encoded calcineurin A, a neuron-specific protein phosphatase catalytic subunit whose striatal expression was upregulated ca. 1.5-fold. SDD may enhance the utility of differential display approaches to identifying regulated genes in tissues in which mRNA complexities are high. (C) 1998 Elsevier Science B.V. C1 NIDA, Mol Neurobiol Branch, Intramural Res Program, Baltimore, MD 21224 USA. Johns Hopkins Univ, Sch Med, Dept Neurol & Neurosci, Baltimore, MD 21224 USA. RP Uhl, GR (reprint author), NIDA, Mol Neurobiol Branch, Intramural Res Program, Box 5180, Baltimore, MD 21224 USA. NR 25 TC 11 Z9 11 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD JAN PY 1998 VL 53 IS 1-2 BP 344 EP 347 DI 10.1016/S0169-328X(97)00333-1 PG 4 WC Neurosciences SC Neurosciences & Neurology GA YZ686 UT WOS:000072280400040 ER PT J AU Azzoli, CG Sagar, M Wu, A Lowry, D Hennings, H Morgan, DL Weinberg, WC AF Azzoli, CG Sagar, M Wu, A Lowry, D Hennings, H Morgan, DL Weinberg, WC TI Cooperation of p53 loss of function and v-Ha-ras in transformation of mouse keratinocyte cell lines SO MOLECULAR CARCINOGENESIS LA English DT Article DE p53; keratinocyte transformation; v-Ha-ras ID WILD-TYPE P53; TEMPERATURE-SENSITIVE MUTANT; TUMOR-SUPPRESSOR GENE; TRANSCRIPTIONAL ACTIVATION; SKIN CARCINOGENESIS; EPIDERMAL-CELLS; TERMINAL DIFFERENTIATION; MALIGNANT PROGRESSION; TRANSGENIC MICE; DNA-DAMAGE AB We previously demonstrated that after transduction with the v-Ha-ras oncogene and grafting onto nude mouse hosts, primary epidermal keratinocytes with a null mutation in the p53 gene form tumors with increased growth rates and predisposition to malignant conversion relative to p53 wild-type keratinocytes (Weinberg WC, et al., Cancer Res 54:5584-5592, 1994). To further explore the cooperation between p53 loss of function and activation of the ras oncogene, cell lines were established from the normal epidermises of newborn and adult p53-null mice, and parallel subclones were reconstituted with the p53(val135) temperature-sensitive mutant. Reconstituted lines C, G, N, and V demonstrated functional p53 transcriptional activator activity at the wild-type-permissive temperature of 32 degrees C, compared with the hygromycin-selected control line X and parental p53-null lines NHK4 and AK1b. Hygromycin-selected subclones, but not the parental lines, made normal skin in vivo; all cell lines made carcinomas after introduction of v-Ha-ras, independent of p53 status. These cell lines were compared in vitro at 32 degrees C to maximize the amount of p53(val135) in the wild-type conformation. Expression of v-Ha-ras did not consistently alter p53-mediated transcriptional activity, suggesting that ras acts downstream or independently of p53. No correlation was observed between p53-mediated transcriptional activity and in vitro growth rates, colony formation after exposure to ultraviolet light, or suppression by normal neighboring keratinocytes. However, keratinocyte cell lines devoid of p53 and expressing v-Ha-ras formed colonies in soft agar; this was blocked at 32 degrees C in all cell lines reconstituted with p53(val135). These keratinocyte lines provide a model for exploring the role of p53 and the interaction of p53 and ras in keratinocyte transformation. (C) 1998 Wiley-Liss, Inc. C1 NCI, Cellular Carcinogenesis & Tumor Promot Lab, NIH, Bethesda, MD 20892 USA. RP Weinberg, WC (reprint author), NCI, Cellular Carcinogenesis & Tumor Promot Lab, NIH, 37 Convent Dr,MSC 4255,Bldg 37,Room 3B25, Bethesda, MD 20892 USA. RI Weinberg, Wendy/A-8920-2009 NR 54 TC 19 Z9 20 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PD JAN PY 1998 VL 21 IS 1 BP 50 EP 61 DI 10.1002/(SICI)1098-2744(199801)21:1<50::AID-MC7>3.0.CO;2-T PG 12 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA YV617 UT WOS:000071845000007 PM 9473771 ER PT J AU Yang, Q Rout, MP Akey, CW AF Yang, Q Rout, MP Akey, CW TI Three-dimensional architecture of the isolated yeast nuclear pore complex: Functional and evolutionary implications SO MOLECULAR CELL LA English DT Article ID SCANNING ELECTRON-MICROSCOPY; SINGLE PARTICLES; MEMBRANE DOMAIN; PROTEIN; ENVELOPE; TRANSLOCATION; TRANSPORT; BINDING; DEPLETION; IMPORT AB We have calculated a three-dimensional map of the yeast nuclear pore complex (yNPC) from frozen-hydrated specimens, thereby providing a direct comparison with the vertebrate NPC. Overall, the smaller yNPC is comprised of an octagonal inner spoke ring that is anchored within the nuclear envelope by a novel membrane-interacting ring. In addition, a cylindrical transporter is located centrally within the spokes and exhibits a variable radial expansion in projection that may reflect gating. The inner spoke ring, a transmembrane spoke domain, and the transporter are conserved between yeast and vertebrates; hence, they are required to form a functional NPC. However, significant alterations in NPC architecture have arisen during evolution that may be correlated with differences in nuclear transport regulation or mitotic behavior. C1 Boston Univ, Sch Med, Dept Biophys, Boston, MA 02118 USA. Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10021 USA. NIH, Endocrinol & Reprod Res Branch, Bethesda, MD 20892 USA. RP Akey, CW (reprint author), Boston Univ, Sch Med, Dept Biophys, Boston, MA 02118 USA. NR 54 TC 245 Z9 248 U1 6 U2 12 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 USA SN 1097-2765 J9 MOL CELL JI Mol. Cell. PD JAN PY 1998 VL 1 IS 2 BP 223 EP 234 DI 10.1016/S1097-2765(00)80023-4 PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA ZG144 UT WOS:000072970500007 PM 9659919 ER PT J AU Appel, JR Johnson, J Narayanan, VL Houghten, RA AF Appel, JR Johnson, J Narayanan, VL Houghten, RA TI Identification of novel antitumor agents from mixture-based synthetic combinatorial libraries using cell-based assays SO MOLECULAR DIVERSITY LA English DT Article DE antitumor agents; cancer drug discovery; combinatorial chemistry; high-throughput screening; mixtures; positional scanning; synthetic combinatorial libraries ID SOLID-PHASE SYNTHESIS; NATIONAL-CANCER-INSTITUTE; DRUG DISCOVERY; HETEROCYCLIC-COMPOUNDS; POOLING STRATEGIES; PEPTIDE LIBRARIES; AMINO-ACIDS; DECONVOLUTION; GENERATION; SCREEN AB A new strategy is presented here which integrates combinatorial library technology with the antitumor in vitro screening system at the National Cancer Institute in the search for novel antitumor agents. Mixture-based synthetic combinatorial libraries (SCLs) representing hundreds of thousands to millions of individual compounds were screened against the cell-based assay, which evaluates compounds for their ability to inhibit the growth of 60 different human tumor cell lines. Five different SCLs, composed of peptides, peptidomimetics, polyamines or small molecules were first tested against three cell lines to identify the most active SCLs. Two SCLs, namely the N-perbenzylated pentamine and the N-acylated permethylated triamine, were deconvoluted to yield individual compounds having significant activities against the 60 tumor cell lines. Active compounds were tested in mice to determine the maximum tolerated dose, followed by in vivo testing in a hollow fiber assay. Using this strategy, three different compounds identified directly from SCLs are currently being evaluated in human tumor xenografts. This study demonstrates for the first time the use of in vitro cell-based assays to identify antitumor lead compounds from mixture-based combinatorial libraries. C1 Torrey Pines Inst Mol Studies, San Diego, CA 92121 USA. NCI, Drug Synth & Chem Branch, Dev Therapeut Program, Bethesda, MD 20892 USA. RP Appel, JR (reprint author), Torrey Pines Inst Mol Studies, 3550 Gen Atom Court, San Diego, CA 92121 USA. NR 40 TC 4 Z9 4 U1 0 U2 1 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 1381-1991 J9 MOL DIVERS JI Mol. Divers. PY 1998 VL 4 IS 2 BP 91 EP 102 DI 10.1023/A:1026441400053 PG 12 WC Biochemistry & Molecular Biology; Chemistry, Applied; Chemistry, Medicinal; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy GA 203TC UT WOS:000080722100002 PM 10425632 ER PT J AU Taniguchi, S Shong, MH Giuliani, C Napolitano, G Saji, M Montani, V Suzuki, K Singer, DS Kohn, LD AF Taniguchi, S Shong, MH Giuliani, C Napolitano, G Saji, M Montani, V Suzuki, K Singer, DS Kohn, LD TI Iodide suppression of major histocompatibility class I gene expression in thyroid cells involves enhancer A and the transcription factor NF-kappa B SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID COMPLEX CLASS-I; SYSTEMIC LUPUS-ERYTHEMATOSUS; DEOXYRIBONUCLEIC-ACID SYNTHESIS; HLA-DR EXPRESSION; GROWTH FACTOR-I; THYROTROPIN RECEPTOR; FRTL-5 CELLS; MAMMALIAN-CELLS; P50 SUBUNIT; INTERFERON AB High concentrations of iodide can induce transient, clinical improvement in patients with autoimmune Graves' disease. Previous work has related this iodide action to the autoregulatory effect of iodide on the growth and function of the thyroid; more recently, we additionally related this to the ability of iodide to suppress major histocompatibility (MHC) class I RNA levels and antigen expression on thyrocytes. In this report, we describe a transcriptional mechanism involved in iodide suppression of class I gene expression, which is potentially relevant to the autoregulatory action of iodide. Transfection experiments in FRTL-5 cells show that iodide decreases class I promoter activity and that this effect can be ascribed to the ability of iodide to modulate the formation of two specific protein/DNA complexes with enhancer A, -180 to -170 bp, of the class 1 5'-flanking region.(1) Thus, iodide decreases the formation of Mod-1, an enhancer A complex involving the p50 subunit of NF-kappa B and a c-fos family member, fra-2, which was previously shown to be important in the suppression of class I levels by hydrocortisone. Unlike hydrocortisone, iodide also increases the formation of a complex with enhancer A, which we show, in antibody shift experiments, is a heterodimer of the p50 and p65 subunits of NF-kappa B. The changes in these complexes are not duplicated by chloride and are related to the action of iodide on class I RNA levels by the following observations. First, FRTL-5 thyroid cells with an aged phenotype coincidentally lose the ability of iodide to decrease MHC class I RNA levels and to induce changes in either complex. Second, the effect of iodide on class I RNA levels and on enhancer A complex formation with Mod-1 and the p50/p65 heterodimer is inhibited by agents that block the inositol phosphate, Ca++, phospholipase A2, arachidonate signal transduction pathway: acetylsalicylate, indomethacin, and 5,8,11,14-eicosatetraynoic acid. Interestingly, iodide can also decrease formation of the Mod-1 complex and increase formation of the complex with the p50/p65 subunits of NF-kappa B when the NF-kappa B enhancer sequence from the Ig kappa light chain, rather than enhancer A, is used as probe; and both actions mimic the action of a phorbol ester. This suggests that iodide may regulate complex formation with NF-kappa B regulatory elements on multiple genes associated with growth and function, providing a potential mechanism relating the autoregulatory action of iodide on thyroid cells and its action on class I gene expression. C1 NIDDKD, Cell Regulat Sect, Metab Dis Branch, NIH, Bethesda, MD 20892 USA. NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA. RP Kohn, LD (reprint author), NIDDKD, Cell Regulat Sect, Metab Dis Branch, NIH, Bldg 10,Room 9C101B, Bethesda, MD 20892 USA. EM lenk@bdg10.niddk.nih.gov RI Saji, Motoyasu/E-4007-2011 NR 63 TC 26 Z9 27 U1 0 U2 2 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 USA SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD JAN PY 1998 VL 12 IS 1 BP 19 EP 33 PG 15 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA YQ406 UT WOS:000071383600002 PM 9440807 ER PT J AU Liu, Y Takeshita, A Nagaya, T Baniahmad, A Chin, WW Yen, PM AF Liu, Y Takeshita, A Nagaya, T Baniahmad, A Chin, WW Yen, PM TI An inhibitory region of the DNA-binding domain of thyroid hormone receptor blocks hormone-dependent transactivation SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID RETINOIC ACID RECEPTORS; TRANSCRIPTIONAL ACTIVATION; LIGAND-BINDING; CO-REPRESSOR; FUNCTIONAL-ANALYSIS; NUCLEAR RECEPTORS; GENE; COACTIVATOR; HETERODIMERS; RECOGNITION AB We have employed a chimeric receptor system in which we cotransfected yeast GAL4 DNA-binding domain/retinoid X receptor beta ligand-binding domain chimeric receptor (GAL4RXR), thyroid hormone receptor-beta (TR beta), and upstream activating sequence-reporter plasmids into CV-1 cells to study repression, derepression, and transcriptional activation. In the absence of T-3, unliganded TR repressed transcription to 20% of basal level, and in the presence of T-3, liganded TR beta derepressed transcription to basal level. Using this system and a battery of TR beta mutants, we found that TR beta/RXR heterodimer formation is necessary and sufficient for basal repression and derepression in this system. Additionally, an AF-2 domain mutant (E457A) mediated basal repression but not derepression, suggesting that interaction with a putative coactivator at this site may be critical for derepression. Interestingly, a mutant containing only the TR beta ligand binding domain (LED) not only mediated derepression, but also stimulated transcriptional activation 10-fold higher than basal level. Studies using deletion and domain swap mutants localized an inhibitory region to the TR beta DNA-binding domain. Titration studies further suggested that allosteric changes promoting interaction with coactivators may account for enhanced transcriptional activity by LED. In summary, our findings suggest that TR heterodimer formation with RXR is important for repression and derepression, and coactivator interaction with the AF-2 domain may be needed for derepression in this chimeric system. Additionally, there may be an inhibitory region in the DNA-binding domain, which reduces TR interaction with coactivators, and prevents full-length wild-type TR beta from achieving transcriptional activation above basal level in this chimeric receptor system. C1 Brigham & Womens Hosp, Dept Med, Div Genet, Boston, MA 02115 USA. Harvard Univ, Sch Med, Boston, MA 02115 USA. Nagoya Univ, Environm Med Res Inst, Dept Endocrinol & Metab, Nagoya, Aichi 464, Japan. Univ Giessen, Genet Inst, D-35392 Giessen, Germany. RP Yen, PM (reprint author), NIDDKD, Mol & Cellular Endocrinol Branch, NIH, Bldg 10,Room 8D12,9000 Rockville Pike, Bethesda, MD 20892 USA. NR 52 TC 12 Z9 12 U1 0 U2 1 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 USA SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD JAN PY 1998 VL 12 IS 1 BP 34 EP 44 PG 11 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA YQ406 UT WOS:000071383600003 PM 9440808 ER PT J AU McKay, LI Cidlowski, JA AF McKay, LI Cidlowski, JA TI Cross-talk between nuclear factor-kappa B and the steroid hormone receptors: Mechanisms of mutual antagonism SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID HUMAN GLUCOCORTICOID RECEPTOR; UBIQUITIN-PROTEASOME PATHWAY; TRANSCRIPTIONAL ACTIVATION; INTERLEUKIN-6 PROMOTER; FUNCTIONAL ANTAGONISM; PROGESTERONE-RECEPTOR; ESTROGEN-RECEPTOR; EXPRESSION; INDUCTION; PROTEIN AB Nuclear factor kappa B (NF-kappa B) is an inducible transcription factor that positively regulates the expression of proimmune and proinflammatory genes, while glucocorticoids are potent suppressors of immune and inflammatory responses. NF-kappa B and the glucocorticoid receptor (GR) physically interact, resulting in repression of NF-kappa B transactivation. In transient cotransfection experiments, we demonstrate a dose-dependent, mutual antagonism between NF-kappa B and GR. Functional dissection of the NF-kappa B p50 and p65 subunits and deletion mutants of GR indicate that the GR antagonism is specific to the p65 subunit of NF-kappa B heterodimer, whereas multiple domains of GR are essential to repress p65-mediated transactivation. Despite its repression of GR transactivation, p65 failed to block the transrepressive GR homologous down-regulation function. We also demonstrate that negative interactions between p65 and GR are not selective for GR, but also occur between NF-kappa B and androgen, progesterone B, and estrogen receptors. However, although each of these members of the steroid hormone receptor family is repressed by NF-kappa B, only GR effectively inhibits p65 transactivation. Further, in cotransfections using a chimeric estrogen-GR, the presence of the GR DNA-binding domain is insufficient to confer mutual antagonism to the p65-estrogen receptor interaction. Selectivity of p65 repression for each steroid receptor is demonstrated by I kappa B rescue from NF-kappa B-mediated inhibition. Together these data suggest that NF-kappa B p65 physically interacts with multiple steroid hormone receptors, and this interaction is sufficient to transrepress each steroid receptor. Further, the NF-kappa B status of a cell has the potential to significantly alter multiple steroid signaling pathways within that cell. C1 NIEHS, Lab Signal Transduct, NIH, Res Triangle Pk, NC 27709 USA. RP Cidlowski, JA (reprint author), NIEHS, Lab Signal Transduct, NIH, POB 12233,MD E2-02, Res Triangle Pk, NC 27709 USA. EM Cidlowski@NIEHS.NIH.GOV NR 42 TC 294 Z9 306 U1 0 U2 8 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 USA SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD JAN PY 1998 VL 12 IS 1 BP 45 EP 56 PG 12 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA YQ406 UT WOS:000071383600004 PM 9440809 ER PT J AU Gangi-Peterson, L Peterson, SN Shapiro, LH Golding, A Caricchio, R Cohen, DI Margulies, DH Cohen, PL AF Gangi-Peterson, L Peterson, SN Shapiro, LH Golding, A Caricchio, R Cohen, DI Margulies, DH Cohen, PL TI bca: an activation-related B-cell gene SO MOLECULAR IMMUNOLOGY LA English DT Article DE B lymphocytes; protein kinases/phosphatases; signal transduction; cellular activation ID PROTEIN-TYROSINE KINASES; PHOSPHORYLATED IG-ALPHA; ANTIGEN RECEPTOR; BINDING-SPECIFICITY; DOMAINS; SH2; COMPLEX; ZAP-70; SYK; ABL AB We have identified a novel activation related B-cell gene (bca) through differential hybridization screening of a murine B cell cDNA library. The deduced amino acid sequence predicted a protein of 482 amino acids with strong sequence similarity to the SH2 and SH3 domains present within the non-catalytic regions of several protein tyrosine kinases. Northern analysis of RNA from several murine B-cell lines revealed a transcript of 1.8 kb, which was not detected in T-cell and non-lymphoid cell lines, bca was transcribed at low levels in resting spleen cells from a variety of normal mouse strains and was strongly expressed in kidney RNA. bca expression was markedly increased in RNA prepared from mitogen activated B cells, and in freshly isolated spleen and lymph node cells of MRL/lpr and NZB autoimmune strains. The unique sequence of bca, which bears no obvious similarity to any specific class of proteins containing SH2 and SH3 domains, suggests that this gene encodes a novel protein potentially involved in B-cell signal transduction. (C) 1998 Elsevier Science Ltd. All rights reserved. C1 Univ N Carolina, Curriculum Genet & Mol Biol, Dept Med, Chapel Hill, NC 27599 USA. Univ N Carolina, Curriculum Genet & Mol Biol, Dept Microbiol Immunol, Chapel Hill, NC 27599 USA. St Jude Childrens Res Hosp, Curriculum Genet & Mol Biol, Memphis, TN 38105 USA. St Jude Childrens Res Hosp, Dept Pharmacol, Memphis, TN 38105 USA. St Jude Childrens Res Hosp, Dept Expt Oncol, Memphis, TN 38105 USA. Johns Hopkins Univ, Sch Med, Baltimore, MD 21205 USA. NCI, NIH, Bethesda, MD 20892 USA. NIAID, Immunol Lab, Bethesda, MD 20892 USA. RP Cohen, PL (reprint author), Univ N Carolina, Curriculum Genet & Mol Biol, Dept Med, Chapel Hill, NC 27599 USA. EM philco@med.unc.edu RI Margulies, David/H-7089-2013; OI Margulies, David/0000-0001-8530-7375; Golding, Amit/0000-0003-3659-6654 FU NIAMS NIH HHS [AR42573, AR33887, P60-AR30701] NR 26 TC 9 Z9 9 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0161-5890 J9 MOL IMMUNOL JI Mol. Immunol. PD JAN PY 1998 VL 35 IS 1 BP 55 EP 63 DI 10.1016/S0161-5890(98)00008-X PG 9 WC Biochemistry & Molecular Biology; Immunology SC Biochemistry & Molecular Biology; Immunology GA ZV994 UT WOS:000074363000006 PM 9683264 ER PT S AU Leon, LR Kozak, W Kluger, MJ AF Leon, LR Kozak, W Kluger, MJ BE Kluger, MJ Bartfai, T Dinarello, CA TI Role of IL-10 in inflammation - Studies using cytokine knockout mice SO MOLECULAR MECHANISMS OF FEVER SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Molecular Mechanisms of Fever CY NOV 02-04, 1997 CL SANTA FE, NEW MEXICO SP NY Acad Sci, Lovelace Resp Res Inst ID PROTECTS MICE; INTERLEUKIN-10; ENDOTOXEMIA AB Interleukin-10 (IL-10) inhibits the synthesis of proinflammatory cytokines known to be involved in fever, including IL-1, IL-6, and tumor necrosis factor-alpha. We hypothesized that IL-10 modulates lipopolysaccharide (LPS)-induced fever in mice. Body temperature was measured by biotelemetry. Swiss Webster mice injected with recombinant murine IL-10 (rmuIL-10) were resistant to fever induced by a low dose of LPS (100 mu g/kg, ip) and to the hypothermic and febrile effects of a high (septic-like) dose of LPS (2.5 mg/kg, ip). Injection of rmuIL-10 alone bad no effect on afebrile body temperature of swiss Webster mice. IL-10 knockout mice showed an exacerbated and prolonged fever in response to a low dose of LPS (50 mu g/kg, ip) compared to their wild-type counterparts. These data support the hypothesis that IL-10 acts as an endogenous cryogen during LPS-induced fever in mice. C1 Lovelace Resp Res Inst, Albuquerque, NM 87185 USA. RP Leon, LR (reprint author), NIDDK, Diabet Branch, NIH, Bldg 10,Room 8N-252,10 Ctr Dr MSC 1770, Bethesda, MD 20892 USA. RI Kozak, Wieslaw/L-5976-2014 FU NIAID NIH HHS [AI-27556] NR 16 TC 34 Z9 35 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-133-2 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 856 BP 69 EP 75 DI 10.1111/j.1749-6632.1998.tb08314.x PG 7 WC Immunology; Multidisciplinary Sciences; Physiology SC Immunology; Science & Technology - Other Topics; Physiology GA BL98W UT WOS:000077375300008 PM 9917866 ER PT S AU Accili, D Kanno, H Kido, Y Lauro, D Rother, KI AF Accili, D Kanno, H Kido, Y Lauro, D Rother, KI BE Takano, K Hizuka, N Takahashi, SI TI Targeted mutations of insulin and IGF-1 receptors in mice. SO MOLECULAR MECHANISMS TO REGULATE THE ACTIVITIES OF INSULIN-LIKE GROWTH FACTORS SE INTERNATIONAL CONGRESS SERIES LA English DT Proceedings Paper CT 4th International Symposium on Insulin-like Growth Factors CY OCT 21-24, 1997 CL TOKYO, JAPAN SP Sumitomo Pharm Co Ltd, Eli Lilly Japan KK, Novo Nordisk Pharma, Japan Chem Res Pharm Co Ltd, Pharmacia & Upjohn, Serono Japan Co Ltd, Fujisawa Pharm Co Ltd, Daiichi Radioisotope Labs Ltd, Cosmic Corp, Eiken Chem Co Ltd, Nikken Chem Co Ltd AB Mice with targeted mutations in the receptors for insulin (IR), IGF-1 (IGF-1R), IGF-2 (IGF-2R), as well as insulin receptor substrate-1 (IRS-1), an important substrate of the IR kinase, have provided important new information on the concerted roles of these proteins. A set of combined mutations in the IR and IGF-IR genes has shed new light onto the physiology of embryonic growth. The phenotype of mice with combined mutations in the insulin and IGF-1R genes suggests that both receptors play important, yet distinct roles to mediate embryonic growth. Interestingly, the growth-promoting actions of IRs during embryogenesis are mediated in response to IGF-2, rather than insulin. C1 NICHHD, Unit Genet & Hormone Act, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA. RP Accili, D (reprint author), NICHHD, Unit Genet & Hormone Act, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA SARA BURGERHARTSTRAAT 25, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0531-5131 BN 0-444-82524-X J9 INT CONGR SER PY 1998 VL 1151 BP 71 EP 78 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA BK87P UT WOS:000073725800009 ER PT S AU Adesanya, OO Zhou, J Samathanam, C Powell-Braxton, L Bondy, CA AF Adesanya, OO Zhou, J Samathanam, C Powell-Braxton, L Bondy, CA BE Takano, K Hizuka, N Takahashi, SI TI IGF-I and uterine growth SO MOLECULAR MECHANISMS TO REGULATE THE ACTIVITIES OF INSULIN-LIKE GROWTH FACTORS SE INTERNATIONAL CONGRESS SERIES LA English DT Proceedings Paper CT 4th International Symposium on Insulin-like Growth Factors CY OCT 21-24, 1997 CL TOKYO, JAPAN SP Sumitomo Pharm Co Ltd, Eli Lilly Japan KK, Novo Nordisk Pharma, Japan Chem Res Pharm Co Ltd, Pharmacia & Upjohn, Serono Japan Co Ltd, Fujisawa Pharm Co Ltd, Daiichi Radioisotope Labs Ltd, Cosmic Corp, Eiken Chem Co Ltd, Nikken Chem Co Ltd C1 NICHD, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA. RP Adesanya, OO (reprint author), NICHD, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA SARA BURGERHARTSTRAAT 25, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0531-5131 BN 0-444-82524-X J9 INT CONGR SER PY 1998 VL 1151 BP 163 EP 168 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA BK87P UT WOS:000073725800019 ER PT S AU Le Roith, D Koval, AP Butler, AA Yakar, S Karas, M Stannard, BS Blakesley, VA AF Le Roith, D Koval, AP Butler, AA Yakar, S Karas, M Stannard, BS Blakesley, VA BE Takano, K Hizuka, N Takahashi, SI TI The insulin-like growth factor-I receptor and cellular signaling: Implications for cellular proliferation and tumorigenesis SO MOLECULAR MECHANISMS TO REGULATE THE ACTIVITIES OF INSULIN-LIKE GROWTH FACTORS SE INTERNATIONAL CONGRESS SERIES LA English DT Proceedings Paper CT 4th International Symposium on Insulin-like Growth Factors CY OCT 21-24, 1997 CL TOKYO, JAPAN SP Sumitomo Pharm Co Ltd, Eli Lilly Japan KK, Novo Nordisk Pharma, Japan Chem Res Pharm Co Ltd, Pharmacia & Upjohn, Serono Japan Co Ltd, Fujisawa Pharm Co Ltd, Daiichi Radioisotope Labs Ltd, Cosmic Corp, Eiken Chem Co Ltd, Nikken Chem Co Ltd C1 NIH, Diabet Branch, Bethesda, MD 20892 USA. RP Le Roith, D (reprint author), NIH, Diabet Branch, 10 Ctr Dr MSC 1770, Bethesda, MD 20892 USA. NR 0 TC 7 Z9 7 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA SARA BURGERHARTSTRAAT 25, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0531-5131 BN 0-444-82524-X J9 INT CONGR SER PY 1998 VL 1151 BP 285 EP 290 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA BK87P UT WOS:000073725800033 ER PT S AU Nissley, P Dey, BR Frick, K Lopaczynski, W Terry, C Furlanetto, RW AF Nissley, P Dey, BR Frick, K Lopaczynski, W Terry, C Furlanetto, RW BE Takano, K Hizuka, N Takahashi, SI TI Differences between insulin and IGF-I signaling SO MOLECULAR MECHANISMS TO REGULATE THE ACTIVITIES OF INSULIN-LIKE GROWTH FACTORS SE INTERNATIONAL CONGRESS SERIES LA English DT Proceedings Paper CT 4th International Symposium on Insulin-like Growth Factors CY OCT 21-24, 1997 CL TOKYO, JAPAN SP Sumitomo Pharm Co Ltd, Eli Lilly Japan KK, Novo Nordisk Pharma, Japan Chem Res Pharm Co Ltd, Pharmacia & Upjohn, Serono Japan Co Ltd, Fujisawa Pharm Co Ltd, Daiichi Radioisotope Labs Ltd, Cosmic Corp, Eiken Chem Co Ltd, Nikken Chem Co Ltd C1 NCI, NIH, Bethesda, MD 20892 USA. RP Nissley, P (reprint author), NCI, NIH, Bldg 10,Rm 4N115, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA SARA BURGERHARTSTRAAT 25, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0531-5131 BN 0-444-82524-X J9 INT CONGR SER PY 1998 VL 1151 BP 291 EP 300 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA BK87P UT WOS:000073725800034 ER PT S AU Cheatham, TE Miller, JL Spector, TI Cieplak, P Kollman, PA AF Cheatham, TE Miller, JL Spector, TI Cieplak, P Kollman, PA BE Leontis, NB SantaLucia, J TI Molecular dynamics simulations on nucleic acid systems using the Cornell et al force field and particle mesh Ewald electrostatics SO MOLECULAR MODELING OF NUCLEIC ACIDS SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Molecular Modeling of Nucleic Acids, at the 213th National Meeting of the American-Chemical-Society CY APR 13-17, 1997 CL SAN FRANCISCO, CALIFORNIA SP Amer Chem Soc, Div Comp Chem ID CIS-SYN PHOTODIMER; CRYSTAL-STRUCTURE; B-DNA; ANTICODON HAIRPIN; AQUEOUS-SOLUTION; THYMINE DIMER; RNA; HELIX; CONFORMATION; TRANSITION AB We present a review of our recent applications of molecular dynamics simulations with explicit inclusion of solvent and neutralizing ions on DNA and RNA systems, using the Cornell et al. molecular mechanics farce field and the particle mesh Ewald approach to describe long range electrostatics. We show that this model does a good job of describing the sequence dependent structural properties of DNA duplexes in aqueous solution, in ethanol and in mixed water-ethanol environments, in the presence of Co(NH3)(6)(3+), in describing the structural properties of phosphoramidate and photochemically damaged DNA duplexes and in describing the properties of RNA duplexes and an RNA hairpin loop. C1 Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA. CombiChem Inc, San Diego, CA 92121 USA. NIH, Struct Biol Lab, Bethesda, MD 20892 USA. Univ San Francisco, Dept Chem, San Francisco, CA 94117 USA. Univ Warsaw, Dept Chem, PL-02093 Warsaw, Poland. RP Cheatham, TE (reprint author), Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA. NR 75 TC 11 Z9 11 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 USA SN 0097-6156 BN 0-8412-3541-4 J9 ACS SYM SER PY 1998 VL 682 BP 285 EP 303 PG 19 WC Chemistry, Multidisciplinary SC Chemistry GA BK18A UT WOS:000071431600017 ER PT S AU Han, YJ Sellers, JR AF Han, YJ Sellers, JR BE Vallee, RB TI Motility assays on molluscan native thick filaments SO MOLECULAR MOTORS AND THE CYTOSKELETON, PT B SE Methods in Enzymology LA English DT Review ID ACTIN-FILAMENTS; SMOOTH-MUSCLE; CALCIUM-BINDING; MYOSIN; PARAMYOSIN; DIRECTION; MOLECULE; SURFACE; FORCES; STEPS C1 NHLBI, Mol Cardiol Lab, NIH, Bethesda, MD 20892 USA. RP NHLBI, Mol Cardiol Lab, NIH, Bethesda, MD 20892 USA. NR 23 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 BN 0-12-182199-4 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1998 VL 298 BP 427 EP 435 DI 10.1016/S0076-6879(98)98038-7 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BL66R UT WOS:000076246300036 PM 9751901 ER PT J AU Qin, ZH Wang, YM Nakai, M Chase, TN AF Qin, ZH Wang, YM Nakai, M Chase, TN TI Nuclear factor-kappa B contributes to excitotoxin-induced apoptosis in rat striatum SO MOLECULAR PHARMACOLOGY LA English DT Article ID METHYL-D-ASPARTATE; PROGRAMMED CELL-DEATH; GENE-EXPRESSION; MOUSE-BRAIN; BINDING ACTIVITIES; DNA FRAGMENTATION; SODIUM-SALICYLATE; MESSENGER-RNA; IN-VITRO; C-JUN AB Excitotoxin-induced destruction of striatal neurons, proposed as a model of Huntington's disease, involves a process having the biochemical stigmata of apoptosis. Recent studies suggested that transcription factor nuclear factor (NF)-kappa B may be involved in excitotoxicity. To further analyze the contribution of NF kappa B to excitotoxic neuronal death in vivo, changes in binding activities of NF kappa B and other transcription factors as well as the consequences of inhibiting NF kappa B nuclear translocation were measured after the infusion of quinolinic acid (120 nmol) into rat striatum. Internucleosomal DNA fragmentation and terminal transferase-mediated dUTP digoxigenin nick end labeling-positive nuclei appeared 12 hr later and intensified over the next 12 hr. NF kappa B binding activity increased severalfold from 2 to 12 hr, then gradually declined during the next 12 hr. Other transcription factor changes included AP-1, whose binding peaked about 6 hr after quinolinic acid administration, and E2F-1, which was only modestly and transiently elevated. In contrast, quinolinic acid lead to a reduction in OCT-I, beginning after 12 hr, and briefly in SP-I binding. The NF kappa B, AP-1, and OCT-I changes were attenuated both by the N-methyl-D-aspartate receptor antagonist MK-801 and the protein synthesis inhibitor cycloheximide. Moreover, quinolinic acid-induced internucleosomal DNA fragmentation and striatal cell death were significantly reduced by the intrastriatal administration of NF kappa B SN50, a cell-permeable recombinant peptide that blocks NF kappa B nuclear translocation. These results illustrate the complex temporal pattern of transcription factor change attending the apoptotic destruction produced in rat striatum by quinolinic acid. They further suggest that NF kappa B activation contributes to the excitotoxin-induced death of striatal neurons. C1 NINDS, ETB, NIH, Bethesda, MD 20892 USA. RP Chase, TN (reprint author), NINDS, ETB, NIH, Bldg 10,Room 5C103,10 Ctr Dr,MSC 1406, Bethesda, MD 20892 USA. EM chase@helix.nih.gov NR 41 TC 150 Z9 163 U1 0 U2 1 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD JAN PY 1998 VL 53 IS 1 BP 33 EP 42 PG 10 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA YT033 UT WOS:000071557300005 PM 9443930 ER PT J AU Verdier-Pinard, P Lai, JY Yoo, HD Yu, JR Marquez, B Nagle, DG Nambu, M White, JD Falck, JR Gerwick, WH Day, BW Hamel, E AF Verdier-Pinard, P Lai, JY Yoo, HD Yu, JR Marquez, B Nagle, DG Nambu, M White, JD Falck, JR Gerwick, WH Day, BW Hamel, E TI Structure-activity analysis of the interaction of curacin A, the potent colchicine site antimitotic agent, with tubulin and effects of analogs on the growth of MCF-7 breast cancer cells SO MOLECULAR PHARMACOLOGY LA English DT Article ID CYANOBACTERIUM LYNGBYA-MAJUSCULA; NATURAL PRODUCT; B-RING; POLYMERIZATION; INHIBITION; SCREEN AB Originally purified as a major lipid component of a strain of the cyanobacterium Lyngbya majuscula isolated in Curacao, curacin A is a potent inhibitor of cell growth and mitosis, binding rapidly and tightly at the colchicine site of tubulin. Because its molecular structure differs so greatly from that of colchicine and other colchicine site inhibitors, we prepared a series of curacin A analogs to determine the important structural features of the molecule. These modifications include reduction and E-to-Z transitions of the olefinic bonds in the 14-carbon side chain of the molecule; disruption of and configurational changes in the cyclopropyl moiety; disruption, oxidation, and configurational reversal in the thiazoline moiety; configurational reversal and substituent modifications at C13; and demethylation at C10. Inhibitory effects on tubulin assembly, the binding of colchicine to tubulin, and the growth of MCF-7 human breast carcinoma cells were examined. The most important portions of curacin A required for its interaction with tubulin seem to be the thiazoline ring and the side chain at least through C4, the portion of the side chain including the C9-10 olefinic bond, and the C10 methyl group. Only two modifications totally eliminated the tubulin-drug interaction. The inactive compounds were a segment containing most of the side chain, including its two substituents, and analogs in which the methyl group at the C13 oxygen atom was replaced by a benzoate residue. Antiproliferative activity comparable with that observed with curacin A was only reproduced in compounds that were potent inhibitors of the binding of colchicine to tubulin. Molecular modeling and quantitative structure-activity relationship studies demonstrated that most active analogs overlapped extensively with curacin A but failed to provide an explanation for the apparent structural analogy between curacin A and colchicine. C1 NCI, Lab Drug Discovery Res & Dev, Dev Therapeut Program,Div Canc Treatment & Diag, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. Univ Texas, SW Med Ctr, Dept Biochem, Dallas, TX 75235 USA. Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75235 USA. Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA. Oregon State Univ, Dept Chem, Corvallis, OR 97331 USA. Univ Pittsburgh, Inst Canc, Dept Environm & Occupat Hlth, Pittsburgh, PA 15238 USA. Univ Pittsburgh, Inst Canc, Dept Pharmaceut Sci, Pittsburgh, PA 15238 USA. RP Hamel, E (reprint author), NIH, Bldg 37,Room 5D02, Bethesda, MD 20892 USA. OI Falck, John/0000-0002-9219-7845 NR 36 TC 184 Z9 186 U1 0 U2 12 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD JAN PY 1998 VL 53 IS 1 BP 62 EP 76 PG 15 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA YT033 UT WOS:000071557300008 PM 9443933 ER PT J AU Licinio, J AF Licinio, J TI Molecular Psychiatry: D'ou venons-nous? Que sommes-nous? Ou allons-nous? SO MOLECULAR PSYCHIATRY LA English DT Editorial Material C1 NIMH, Clin Neuroendocrinol Branch, NIH, Bethesda, MD 20892 USA. RP Licinio, J (reprint author), NIMH, Clin Neuroendocrinol Branch, NIH, Bldg 10,Rm 2D46, Bethesda, MD 20892 USA. RI Licinio, Julio/L-4244-2013 OI Licinio, Julio/0000-0001-6905-5884 NR 3 TC 0 Z9 0 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 1359-4184 J9 MOL PSYCHIATR JI Mol. Psychiatr. PD JAN PY 1998 VL 3 IS 1 BP 4 EP 5 DI 10.1038/sj.mp.4000356 PG 2 WC Biochemistry & Molecular Biology; Neurosciences; Psychiatry SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Psychiatry GA ZL776 UT WOS:000073470400001 ER PT J AU Malhotra, AK Goldman, D Buchanan, RW Rooney, W Clifton, A Kosmidis, MH Breier, A Pickar, D AF Malhotra, AK Goldman, D Buchanan, RW Rooney, W Clifton, A Kosmidis, MH Breier, A Pickar, D TI The dopamine D-3 receptor (DRD3) Ser(9)Gly polymorphism and schizophrenia: a haplotype relative risk study and association with clozapine response SO MOLECULAR PSYCHIATRY LA English DT Article DE polymorphism; dopamine 3 receptor; schizophrenia; clozapine; genetics; association ID D3 RECEPTOR; GENE; HOMOZYGOSITY AB Several lines of evidence suggest that the dopamine D-3 receptor is involved in the pathophysiology of schizophrenia.(1-3) The D-3 receptor gene (DRD3) contains a polymorphism resulting in a serine-glycine substitution in the N-terminus of the receptor.(4) Shaikh and colleagues(5) have reported a significant association between the DRD3 Ser, allele and the Ser(9)/Ser(9) genotype with schizophrenia in 133 Caucasians. In a meta-analysis of previous studies, Ser(9) and the Ser(9)/Ser(9) genotype were found to be significantly associated with schizophrenia, although these investigators could not confirm reports(6,7) of excess homozygosity at this locus in schizophrenia. These authors also report that, in an unblinded study, the Ser(9)/Ser(9) genotype was more frequent in patients who did not respond to clozapine, These data represent the most comprehensive examination of DRD3 Ser(9)Gly in schizophrenia to date, We have therefore determined DRD3 Ser(9)Gly genotypes in 58 patients with schizophrenia and in their parents. Moreover, we have genotyped 68 schizophrenics participating in double-blind clozapine trials, We do not find that Ser(9) is preferentially transmitted in schizophrenia, cannot confirm excess DRD3 homozygosity in schizophrenia, and do not replicate the association between DRD3 and clozapine response. These data suggest that allelic variation in DRD3 may not play a role in the pathophysiology of schizophrenia or in clozapine response. C1 NIMH, Expt Therapeut Branch, NIH, Bethesda, MD 20892 USA. NIAAA, Neurogenet Lab, NIH, Rockville, MD 20852 USA. Univ Maryland, Maryland Psychiat Res Ctr, Dept Psychiat, Catonsville, MD 21228 USA. RP Malhotra, AK (reprint author), NIMH, Expt Therapeut Branch, NIH, 10 Ctr Dr,Bldg 10,Room 4N212, Bethesda, MD 20892 USA. RI Goldman, David/F-9772-2010 OI Goldman, David/0000-0002-1724-5405 NR 18 TC 71 Z9 75 U1 3 U2 3 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 1359-4184 J9 MOL PSYCHIATR JI Mol. Psychiatr. PD JAN PY 1998 VL 3 IS 1 BP 72 EP 75 DI 10.1038/sj.mp.4000288 PG 4 WC Biochemistry & Molecular Biology; Neurosciences; Psychiatry SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Psychiatry GA ZL776 UT WOS:000073470400016 PM 9491816 ER PT J AU Swift, RG Polymeropoulos, MH Torres, R Swift, M AF Swift, RG Polymeropoulos, MH Torres, R Swift, M TI Predisposition of Wolfram syndrome heterozygotes to psychiatric illness SO MOLECULAR PSYCHIATRY LA English DT Article DE Wolfram syndrome; heterozygotes; psychiatric illness ID CYSTIC-FIBROSIS; GENE; SIBLINGS; DISORDER; LINKAGE AB Identification of specific genes that predispose to psychiatric illness will lead to more precise psychiatric diagnosis and more effective treatment. Heterozygous carriers of genes for many autosomal recessive syndromes may be 1% or more of the general population. Thus, if mutations at a specific locus produce psychiatric manifestations in homozygous affected individuals, it is important to determine whether mutations at such a locus also predispose heterozygous carriers to psychiatric disorders. The hypothesis that heterozygous carriers of the gene for the Wolf ram syndrome (WS) are predisposed to psychiatric illness was supported previously by the finding of an excess of psychiatric hospitalizations and suicides in WS blood relatives compared to spouse controls.(1) This hypothesis has now been tested further by comparing the number of psychiatrically hospitalized blood relatives with the specific marker haplotype associated with the Wolf ram syndrome gene in their families to the number expected under the null hypothesis, calculated from Mendelian inheritance principles and the estimated haplotype frequency.(2) The proportion of psychiatrically hospitalized relatives who were WS carriers (10/11) was much higher than expected (3.1/11), leading to the provisional estimate that WS gene carriers are 26-fold more likely to require psychiatric hospitalization than non-carriers. C1 New York Med Coll, Inst Genet Anal Common Dis, Dept Psychiat, Hawthorne, NY USA. New York Med Coll, Inst Genet Anal Common Dis, Dept Pediat, Hawthorne, NY USA. Natl Human Genome Res Inst, Gene Mapping Unit, Lab Genet Dis Res, NIH, Bethesda, MD 20892 USA. RP Swift, M (reprint author), New York Med Coll, Inst Genet Anal Common Dis, Dept Psychiat, 4 Skyline Dr, Hawthorne, NY USA. NR 21 TC 83 Z9 85 U1 1 U2 2 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 1359-4184 J9 MOL PSYCHIATR JI Mol. Psychiatr. PD JAN PY 1998 VL 3 IS 1 BP 86 EP 91 DI 10.1038/sj.mp.4000344 PG 6 WC Biochemistry & Molecular Biology; Neurosciences; Psychiatry SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Psychiatry GA ZL776 UT WOS:000073470400019 PM 9491819 ER PT S AU Miyamoto, S Katz, BZ Lafrenie, RM Yamada, KM AF Miyamoto, S Katz, BZ Lafrenie, RM Yamada, KM BE Fleischmajer, R Timpl, R Werb, Z TI Fibronectin and integrins in cell adhesion, signaling, and morphogenesis SO MORPHOGENESIS: CELLULAR INTERACTIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Morphogenesis - Cellular Interactions CY OCT 20-23, 1997 CL BETHESDA, MARYLAND SP New York Acad Sci, March Dimes Birth Defects Fdn, Natl Inst Child Hlth & Human Dev, Council Tobacco Res, USA Inc, Fdn Basic Cutaneous Res, Genome Therapeut Corp, Ontogeny Inc, Orentreich Fdn Advancement Sci Inc ID SITE-DIRECTED MUTAGENESIS; BINDING DOMAIN; SYNTHETIC PEPTIDES; BETA-3 INTEGRINS; RECEPTOR; MATRIX; TRANSDUCTION; GASTRULATION; PHOSPHORYLATION; ACTIVATION AB Fibronectin and integrins play crucial roles in a variety of morphogenetic processes, in which they mediate cell adhesion, migration, and signal transduction. They induce hierarchical transmembrane organization of cytoskeletal and signaling molecules into multimolecular complexes of more than 30 proteins. Organization of these complexes is a synergistic process dependent on integrin aggregation anti occupancy, as well as tyrosine phosphorylation. Integrins also cooperate with growth-factor receptors to enhance signaling. Fibronectin and integrins induce a variety of downstream effects, including enhanced transcription factor activity, induction of over 30 genes (>half novel), and altered expression of over 100 proteins. Fibronectin and integrins therefore trigger a hierarchy of signaling responses involved in regulating processes crucial for normal morphogenesis, including cell adhesion, migration, and specific gene expression. C1 NIDR, Craniofacial Dev Biol & Regenerat Branch, NIH, Bethesda, MD 20892 USA. Kyushu Natl Canc Ctr, Minami Ku, Fukuoka 815, Japan. NE Ontario Reg Canc Ctr, Sudbury, ON P3E 5J1, Canada. RP Yamada, KM (reprint author), NIDR, Craniofacial Dev Biol & Regenerat Branch, NIH, Bldg 30,Room 421,30 Convent Dr MSC 4370, Bethesda, MD 20892 USA. OI Yamada, Kenneth/0000-0003-1512-6805 NR 45 TC 156 Z9 160 U1 0 U2 7 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-131-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 857 BP 119 EP 129 DI 10.1111/j.1749-6632.1998.tb10112.x PG 11 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Science & Technology - Other Topics GA BL93V UT WOS:000077211500011 PM 9917837 ER PT S AU Fleischmajer, R Perlish, JS Macdonald, ED Schechter, A Murdoch, AD Iozzo, RV Yamada, Y AF Fleischmajer, R Perlish, JS Macdonald, ED Schechter, A Murdoch, AD Iozzo, RV Yamada, Y BE Fleischmajer, R Timpl, R Werb, Z TI There is binding of collagen IV to beta 1 integrin during early skin basement membrane assembly SO MORPHOGENESIS: CELLULAR INTERACTIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Morphogenesis - Cellular Interactions CY OCT 20-23, 1997 CL BETHESDA, MARYLAND SP New York Acad Sci, March Dimes Birth Defects Fdn, Natl Inst Child Hlth & Human Dev, Council Tobacco Res, USA Inc, Fdn Basic Cutaneous Res, Genome Therapeut Corp, Ontogeny Inc, Orentreich Fdn Advancement Sci Inc ID HEPARAN-SULFATE PROTEOGLYCAN; BASAL LAMINA; IN-VITRO; DEVELOPING INTESTINE; NIDOGEN; EXPRESSION; MODEL; FIBROBLASTS; DEPOSITION; CHAINS AB This study is concerned with the mechanism of basement membrane assembly in an in vitro 3-dimensional skin-culture system. Dermal fibroblasts alone can synthesize collagen IV, perlecan, and nidogen, but cannot assemble them into a basement membrane. When keratinocytes are added to the culture, however,linear assembly of collagen TV, perlecan, and nidogen is noted at the epidermo-dermal interface. Northern blots and in situ hybridization showed that perlecan and nidogen mRNAs derive exclusively from fibroblasts, while the alpha 2 (TV) collagen chain is expressed by both keratinocytes and fibroblasts, although the major source is in the mesenchyma (80%). Prior to the development of the lamina densa, collagen IV colocalizes with beta 1 integrins, most likely alpha 1 beta 1 and alpha 2 beta 1, which are known receptors for this collagen. Blocking experiments with the AIlB2 mAb (anti-beta 1 integrin subunit) and by peptide inhibition with the CB3(TV) collagen fragment disrupted the assembly of collagen IV. This study suggests that the initiation of basement-membrane formation involves binding of collagen IV molecules to keratinocyte cell-matrix integrins. These complexes act as nucleation sites for further polymerization of collagen IV molecules mostly derived from fibroblasts, by a process of self-assembly. C1 CUNY Mt Sinai Sch Med, Dept Dermatol, New York, NY 10029 USA. Thomas Jefferson Univ, Dept Pathol & Cell Biol, Philadelphia, PA 19107 USA. NIDR, Craniofacial Dev Biol & Regenerat Branch, NIH, Bethesda, MD 20892 USA. RP Fleischmajer, R (reprint author), CUNY Mt Sinai Sch Med, Dept Dermatol, 1 Gustav L Levy Pl, New York, NY 10029 USA. EM RF@doc.mssm.ed.w; RF@doc.mssm.ed.w NR 36 TC 34 Z9 34 U1 0 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-131-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 857 BP 212 EP 227 DI 10.1111/j.1749-6632.1998.tb10118.x PG 16 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Science & Technology - Other Topics GA BL93V UT WOS:000077211500017 PM 9917843 ER PT J AU Hallett, M AF Hallett, M TI Overview of human tremor physiology SO MOVEMENT DISORDERS LA English DT Article; Proceedings Paper CT Symposium of the Movement-Disorder-Society CY JUL 11-12, 1997 CL KIEL, GERMANY SP Movement Disorder Soc, German Soc Clin Neurophysiol, Tremor Fdn DE accelerometry; electromyography; mechanical oscillator; central oscillator; physiological tremor; essential tremor; rest tremor; palatal tremor ID SUBJECTS MIMICKING TREMOR; PARKINSONS-DISEASE; MAGNETIC STIMULATION; CORTICAL TREMOR; MYOCLONUS; NEUROPATHY; MODULATION; WRIST; EMG AB The physiology differs in the many forms of human tremor. Tremors may derive from mechanical oscillations, mechanical reflex oscillations, normal central oscillators, and pathologic central oscillators. Methods of studying tremor include accelerometry and electromyography (EMG). An excellent method consists of accelerometry and EMG combined with spectral analysis and weighting of the body part, which allows separation of tremors coming from mechanical reflex and central oscillators. Physiologic tremor is a mechanical tremor with a possible contribution of the normal 8-12 Hz central oscillator; exaggerated physiologic tremor is a mechanical reflex tremor. Essential tremor (ET) comes from a central oscillator that can be easily influenced with sensory input. The classic rest tremor of Parkinson's disease (PD) comes from a central oscillator that seems less easily influenced with sensory input but can be affected by transcranial magnetic stimulation. Other tremors with central oscillators are palatal tremor and orthostatic tremor. Other tremors whose physiology involves central loops includes cerebellar tremor and cortical tremor. Neuropathic tremors may be a result of delays in peripheral loops. but central oscillators play a role in some. C1 NINDS, Bethesda, MD 20892 USA. RP Hallett, M (reprint author), NINDS, Bldg 10,Room 5N226,10 Ctr Dr MSC 1428, Bethesda, MD 20892 USA. NR 19 TC 50 Z9 50 U1 0 U2 6 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0885-3185 J9 MOVEMENT DISORD JI Mov. Disord. PY 1998 VL 13 SU 3 BP 43 EP 48 PG 6 WC Clinical Neurology SC Neurosciences & Neurology GA 135JA UT WOS:000076797500007 PM 9827594 ER PT B AU Brewer, HB AF Brewer, HB BE Gotto, AM Lenfant, C Paoletti, R Catapano, AL Jackson, AS TI New insights into the role of HDL in the development of cardiovascular disease SO MULTIPLE RISK FACTORS IN CARDIOVASCULAR DISEASE: STRATEGIES OF PREVENTION OF CORONARY HEART DISEASE, CARDIAC FAILURE, AND STROKE SE MEDICAL SCIENCE SYMPOSIA SERIES LA English DT Proceedings Paper CT 4th International Symposium on Multiple Risk Factors in Cardiovascular Disease - Strategies of Prevention of Coronary Heart Disease, Cardiac Failure, and Stroke CY APR 23-25, 1997 CL WASHINGTON, D.C. C1 NHLBI, Mol Dis Branch, NIH, Bethesda, MD 20892 USA. RP Brewer, HB (reprint author), NHLBI, Mol Dis Branch, NIH, Bldg 10,Room 7N115,10 Ctr Dr MSC 1666, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS BN 0-7923-5023-5 J9 MED SCI SYMP SER PY 1998 VL 12 BP 1 EP 7 PG 7 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA BK92U UT WOS:000073874700001 ER PT J AU Panizza, M Nilsson, J Roth, BJ Grill, SE Demirci, M Hallett, M AF Panizza, M Nilsson, J Roth, BJ Grill, SE Demirci, M Hallett, M TI Differences between the time constant of sensory and motor peripheral nerve fibers: Further studies and considerations SO MUSCLE & NERVE LA English DT Article DE neural time constant; single axons; motor fibers; sensory fibers; magnetic stimulation ID STIMULUS-DURATION; STIMULATION; SENSITIVITY AB Using a method of latent addition, we previously demonstrated that sensory fibers had time constants that were about three times longer than those of motor fibers. The aim of the present work was to confirm this difference by determining the time constants for single sensory axons by using microneurography and for single motor axons by recording single motor units with bipolar concentric needle electrodes. To determine the influence of the conditioning pulse on the neural time constant, we used both depolarizing and hyperpolarizing conditioning pulses, When hyperpolarizing conditioning pulses at comparable intensity were applied, the tendency was to find shorter time constants than when depolarizing pulses were applied, although still with the motor time constant being slightly shorter. Although the absolute values varied with the different methods, the sensory time constant was generally three times the motor time constant for depolarizing conditioning stimuli, whereas for hyperpolarizing conditioning stimuli the difference dropped to about one and a half. These characteristics improve understanding of the behavior of sensory and motor axons, and, in particular, explain the differential excitability. Determination of neural time constants might prove valuable for clinical use. (C) 1998 John Wiley & Sons, Inc. C1 IRCCS, Rehabil Inst Castel Goffredo, Salvatore Maugeri Fdn, Clin Neurophysiol Lab, I-46042 Castel Goffredo, MN, Italy. Vanderbilt Univ, Dept Phys & Astron, Nashville, TN 37235 USA. NINDS, Human Motor Control Sect, NIH, Bethesda, MD 20892 USA. RP Panizza, M (reprint author), IRCCS, Rehabil Inst Castel Goffredo, Salvatore Maugeri Fdn, Clin Neurophysiol Lab, Via Osped 36, I-46042 Castel Goffredo, MN, Italy. RI Roth, Bradley/A-4920-2008; Demirci, Mehmet/D-7081-2012 OI Demirci, Mehmet/0000-0002-7316-3877 NR 16 TC 21 Z9 21 U1 0 U2 4 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0148-639X J9 MUSCLE NERVE JI Muscle Nerve PD JAN PY 1998 VL 21 IS 1 BP 48 EP 54 DI 10.1002/(SICI)1097-4598(199801)21:1<48::AID-MUS7>3.0.CO;2-G PG 7 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA YP688 UT WOS:000071304000009 PM 9427223 ER PT J AU Koffman, BM Rugiero, M Dalakas, MC AF Koffman, BM Rugiero, M Dalakas, MC TI Immune-mediated conditions and antibodies associated with sporadic inclusion body myositis SO MUSCLE & NERVE LA English DT Article DE inclusion body myositis; autoimmune diseases; autoantibodies ID IDIOPATHIC INFLAMMATORY MYOPATHY; SJOGRENS-SYNDROME; CELLS AB We reviewed 99 patients with sporadic inclusion body myositis (IBM), searching for a coexisting autoimmune disease, other conditions with altered immune function, or the presence of autoantibodies. Thirteen patients had one or more of 11 diseases with altered immune function. Forty-three patients had elevated titers of one or more of nine different, albeit nondisease-specific, autoantibodies. Twenty-five patients had dysproteinemia or dysproteinuria. We conclude that IBM is frequently associated with systemic immune disorders or nonspecific autoantibodies. Although aging may explain some of these phenomena, an altered immune function need to be considered in the pathogenesis of IBM. (C) 1998 John Wiley & Sons, Inc. C1 NINDS, Neuromuscular Dis Sect, NIH, Bethesda, MD 20892 USA. RP Dalakas, MC (reprint author), NINDS, Neuromuscular Dis Sect, NIH, 10 Ctr Dr,MSC-1382, Bethesda, MD 20892 USA. NR 25 TC 70 Z9 70 U1 0 U2 0 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0148-639X J9 MUSCLE NERVE JI Muscle Nerve PD JAN PY 1998 VL 21 IS 1 BP 115 EP 117 DI 10.1002/(SICI)1097-4598(199801)21:1<115::AID-MUS15>3.0.CO;2-2 PG 3 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA YP688 UT WOS:000071304000017 PM 9427231 ER PT J AU Dillon, D Combes, R Zeiger, E AF Dillon, D Combes, R Zeiger, E TI The effectiveness of Salmonella strains TA100, TA102 and TA104 for detecting mutagenicity of some aldehydes and peroxides SO MUTAGENESIS LA English DT Article ID BACTERIAL MUTATION ASSAYS; A.T BASE-PAIRS; OXIDATIVE MUTAGENS; ESCHERICHIA-COLI; RISK ASSESSMENT; TYPHIMURIUM; CHEMICALS; TESTS; CINNAMALDEHYDE; FORMALDEHYDE AB Several aldehydes and peroxides were tested for mutagenicity using Salmonella typhimurium tester strains TA97a, TA100, TA102 and TA104, in the presence and absence of Aroclor-induced liver S9 mix from F344 rats and B6C3F(1) mice, in either preincubation or vapour phase protocols, Some chemicals were tested in additional Salmonella strains. Benzaldehyde, butyraldehyde, benzoyl peroxide, 4-chlorobenzaldehyde, isobutyraldehyde, propionaldehyde and veratraldehyde were non-mutagenic, Acetaldehyde and dicumyl peroxide gave inconsistent results and furfural gave equivocal responses in TA100 and TA104. Cumene hydroperoxide, formaldehyde and glutaraldehyde were mutagenic in TA100, TA102 and TA104, trans-Cinnamaldehyde exhibited a weak mutagenic response in TA100 with mouse liver S9 only, 2,4,5-Trimethoxybenzaldehyde was mutagenic only in strain TA1538 with rat liver S9. With the exception of butanone peroxide, which was mutagenic only in TA104, all chemicals mutagenic in strains TA102 and/or TA104 were also mutagenic in TA100, The data do not, therefore, support the preferential use of strains TA102 and TA104 for screening aldehydes and peroxides for mutagenicity, For a number of these chemicals the advantages of using TA102 or TA104 was in the increased responses compared with those obtained with TA100, Two of the four peroxides were mutagenic and one of these was mutagenic only with TA104. This suggests that strains TA102 and TA104 be used if peroxides are not mutagenic in TA100 or TA97. C1 NIEHS, Res Triangle Pk, NC 27709 USA. Inveresk Res Int Ltd, Tranent EH33 2NE, Scotland. RP Zeiger, E (reprint author), NIEHS, POB 12233, Res Triangle Pk, NC 27709 USA. FU NIEHS NIH HHS [N01-ES-05298] NR 46 TC 44 Z9 47 U1 0 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0267-8357 J9 MUTAGENESIS JI Mutagenesis PD JAN PY 1998 VL 13 IS 1 BP 19 EP 26 DI 10.1093/mutage/13.1.19 PG 8 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA YV077 UT WOS:000071787300003 PM 9491389 ER PT S AU Penn, AS Low, BW Jaffe, IA Luo, L Jacques, JJ AF Penn, AS Low, BW Jaffe, IA Luo, L Jacques, JJ BE Richman, DP TI Drug-induced autoimmune myasthenia gravis SO MYASTHENIA GRAVIS AND RELATED DISEASES: DISORDERS OF THE NEUROMUSCULAR JUNCTION SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT 9th International Conference on Myasthenia Gravis and Related Disorders CY MAY 07-10, 1997 CL SANTA MONICA, CA SP New York Acad Sci, Myasthenia Gravis Fdn Amer Inc, Calif Chapter, Natl Inst Neurol Disorders & Stroke, Bayer Corp, Pharm Div, ICN Pharm, Jerome Fdn, Olsten Hlth Serv, Ultracare Home Hlth, Alpha Therapeut Serv, Broadway Fed Saving & Loan Assoc, Home Savings Bank Amer, Long Beach Branch, Ironworkers Local 433, Los Angeles, Calif, La Jolla Pharm Co, Merrill Lynch Pierce Fenner & Smith, Myasthenia Fdn Amer Inc, Orange Cty Auxiliary, Calif Chapter, San Gabriel Valley Auxiliary, Calif Chapter, Wellcome Trust ID PENICILLAMINE-INDUCED MYASTHENIA; BROWN-NORWAY RATS; T-CELL RESPONSE; ACETYLCHOLINE-RECEPTOR; ANTINUCLEAR ANTIBODIES; RHEUMATOID-ARTHRITIS; PATHO-PHYSIOLOGY; AUTOANTIBODIES; INDUCTION; CAPTOPRIL C1 NINDS, NIH, Bethesda, MD 20892 USA. Columbia Univ Coll Phys & Surg, Dept Neurol, New York, NY 10032 USA. Columbia Univ Coll Phys & Surg, Dept Biochem & Mol Biophys, New York, NY 10032 USA. Columbia Univ Coll Phys & Surg, Dept Med, New York, NY 10032 USA. RP Penn, AS (reprint author), NINDS, NIH, 31 Ctr Dr, Bethesda, MD 20892 USA. FU NINDS NIH HHS [NS17904] NR 52 TC 19 Z9 22 U1 0 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-119-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 841 BP 433 EP 449 DI 10.1111/j.1749-6632.1998.tb10961.x PG 17 WC Multidisciplinary Sciences; Clinical Neurology; Neurosciences SC Science & Technology - Other Topics; Neurosciences & Neurology GA BL26B UT WOS:000074924900058 PM 9668273 ER PT J AU Hallock, YF Cardellina, JH Boyd, MR AF Hallock, YF Cardellina, JH Boyd, MR TI (-)-Frondosins A and D, HIV-inhibitory sesquiterpene hydroquinone derivatives from Euryspongia sp. SO NATURAL PRODUCT LETTERS LA English DT Article DE HIV-inhibitory; sesquiterpene hydroquinone; Euryspongia; frondosin ID QUINONES; STEREOCHEMISTRY AB (-)-Frondosins A and D, two sesquiterpene hydroquinone metabolites, were isolated and identified from the HIV-inhibitory organic extracts of the marine sponge Euryspongia sp. In contrast to the decalin rings found previously in bicyclic marine sesquiterpene quinones and quinols, (-)-frondosins A and D possess the less common bicyclo[5.4.0]undecane sesquiterpene ring system. C1 NCI, Lab Drug Discovery Res & Dev, Dev Therapeut Program, Div Canc Treatment Diag & Ctr, Frederick, MD 21702 USA. NR 24 TC 46 Z9 47 U1 1 U2 8 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1057-5634 J9 NAT PROD LETT JI Nat. Prod. Lett. PY 1998 VL 11 IS 2 BP 153 EP 160 DI 10.1080/10575639808041212 PG 8 WC Biochemistry & Molecular Biology; Chemistry, Medicinal SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy GA YX928 UT WOS:000072094700011 ER PT J AU Bokesch, HR Young, SM McKee, TC Blunt, JW Boyd, MR AF Bokesch, HR Young, SM McKee, TC Blunt, JW Boyd, MR TI Lambertianoside, a novel phenylglycoside from Eugenia lambertiana SO NATURAL PRODUCT LETTERS LA English DT Article DE lambertianoside; Eugenia lambertiana; phenylglycoside ID GLYCOSIDES AB A novel phenylglycoside has been isolated from the aqueous extract of leaves from Eugenia lambertiana. C1 NCI, Frederick Canc Res & Dev Ctr, Lab Drug Discovery Res & Dev, Dev Therapeut Program,Div Canc Treatment & Diag, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC, Frederick, MD 21702 USA. NR 12 TC 1 Z9 1 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1057-5634 J9 NAT PROD LETT JI Nat. Prod. Lett. PY 1998 VL 11 IS 3 BP 211 EP 216 DI 10.1080/10575639808044949 PG 6 WC Biochemistry & Molecular Biology; Chemistry, Medicinal SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy GA ZH575 UT WOS:000073125200009 ER PT J AU Johnson, HA Rogers, LL Alkire, ML McCloud, TG NcLaughlin, JL AF Johnson, HA Rogers, LL Alkire, ML McCloud, TG NcLaughlin, JL TI Bioactive monoterpenes from Monarda fistulosa (Lamiaceae) SO NATURAL PRODUCT LETTERS LA English DT Article DE Monarda fistulosa; cytotoxic; pesticidal; thymoquinone; thymol; thymohydroquinone ID TUMOR-CELL-LINES; FEASIBILITY AB Bioactivity-guided fractionation of Monarda fistulosa L. (F005), using the brine shrimp lethality test (BST) and crown gall tumor potato disc assay CPD), led to the isolation of the bioactive monoterpenes, thymoquinone (1), and thymol (2). 1, surprisingly, exhibited selective cytotoxicities against certain eel lines. Of over 60 human tumor cell lines evaluated, 1 was very selective for SF-539 (CNS) with an LC50 < 2.51 x 10(-2) mu g/ml. Other cell lines targeted by 1 were PC-3 (prostate), M-14 (melanoma), OVCAR-5 (ovarian), and MCF-7 (breast). The reduction of 1 to thymohydroquinone (3) resulted in a 1.7 fold decrease in the cytotoxic potency for PC-3. Thymol (2), although not significantly cytotoxic, demonstrated strong pesticidal activity, more potent than rotenone, in the yellow fever mosquito larvae assay (YFM). None of the compounds, 1-3, approached the IC50 value of rotenone in inhibiting oxygen uptake in the rat liver mitochondrial bioassay, suggesting that they must have another mode of action. C1 Purdue Univ, Sch Pharm & Pharmacal Sci, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA. NCI, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NR 15 TC 15 Z9 15 U1 1 U2 13 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1057-5634 J9 NAT PROD LETT JI Nat. Prod. Lett. PY 1998 VL 11 IS 4 BP 241 EP 250 DI 10.1080/10575639808044955 PG 10 WC Biochemistry & Molecular Biology; Chemistry, Medicinal SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy GA ZN776 UT WOS:000073681500001 ER PT J AU Bais, C Santomasso, B Coso, O Arvanitakis, L Raaka, EG Gutkind, JS Asch, AS Cesarman, E Gerhengorn, MC Mesri, EA AF Bais, C Santomasso, B Coso, O Arvanitakis, L Raaka, EG Gutkind, JS Asch, AS Cesarman, E Gerhengorn, MC Mesri, EA TI G-protein-coupled receptor of Kaposi's sarcoma-associated herpesvirus is a viral oncogene and angiogenesis activator SO NATURE LA English DT Article ID ENDOTHELIAL GROWTH-FACTOR; DNA-SEQUENCES; CELLS; EXPRESSION; VIRUS AB The Kaposi's sarcoma-associated herpesvirus (KSHV/HHV8) is a gamma-2 herpesvirus(1-5) that is implicated in the pathogenesis of Kaposi's sarcoma(1,5) and of primary effusion B-cell lymphomas (PELs)(6). KSHV infects malignant and progenitor cells of Kaposi's sarcoma(7) and PEL2,6,8, it encodes putative oncogenes(4,5,9) and genes that may cause Kaposi's sarcoma pathogenesis by stimulating angiogenesis(4,5,9,10). The G-protein-coupled receptor encoded by an open reading frame (ORF 74) of KSHV9 is expressed in Kaposi's sarcoma lesions and in PEL9,11 and stimulates signalling pathways linked to cell proliferation(12) in a constitutive (agonist-independent) way(12). Here we show that signalling by this KSHV G-protein-coupled receptor leads to cell transformation and tumorigenicity, and induces a switch to an angiogenic phenotype(13) mediated by vascular endothelial growth factor(14), an angiogenesis(13,14) and Kaposi's-spindle-cell growth factor(15-17). We find that this receptor can activate two protein kinases, JNK/SAPK and p38MAPK, by triggering signalling cascades like those induced by inflammatory cytokines(18) that are angiogenesis activators(19) and mitogens for Kaposi's sarcoma cells(10) and B cells. We conclude that the KSHV G-protein-coupled receptor is a viral oncogene that can exploit cell signalling pathways to induce transformation and angiogenesis in KSHV-mediated oncogenesis. C1 Cornell Univ, Coll Med, Lab Viral Oncogenesis, New York, NY 10021 USA. Cornell Univ, Coll Med, Div Hematol Oncol, New York, NY 10021 USA. Cornell Univ, Coll Med, Dept Med, Div Mol Med, New York, NY 10021 USA. Cornell Univ, Coll Med, Dept Pathol, New York, NY 10021 USA. NIDR, Mol Signaling Unit, Cellular Dev & Oncol Lab, NIH, Bethesda, MD 20892 USA. RP Mesri, EA (reprint author), Cornell Univ, Coll Med, Lab Viral Oncogenesis, New York, NY 10021 USA. RI Gutkind, J. Silvio/A-1053-2009 NR 30 TC 620 Z9 639 U1 2 U2 16 PU MACMILLAN MAGAZINES LTD PI LONDON PA PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW SN 0028-0836 J9 NATURE JI Nature PD JAN 1 PY 1998 VL 391 IS 6662 BP 86 EP 89 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA YP888 UT WOS:000071326100054 PM 9422510 ER PT J AU Dixon, SC Horti, J Guo, Y Reed, E Figg, WD AF Dixon, SC Horti, J Guo, Y Reed, E Figg, WD TI Methods for extracting and amplifying genomic DNA isolated from frozen serum SO NATURE BIOTECHNOLOGY LA English DT Editorial Material ID AMPLIFICATION; PURIFICATION; LINKAGE C1 NCI, Med Branch, NIH, Bethesda, MD 20892 USA. RI Figg Sr, William/M-2411-2016 NR 14 TC 38 Z9 42 U1 1 U2 3 PU NATURE AMERICA INC PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1087-0156 J9 NAT BIOTECHNOL JI Nat. Biotechnol. PD JAN PY 1998 VL 16 IS 1 BP 91 EP 94 DI 10.1038/nbt0198-91 PG 4 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA YP708 UT WOS:000071306000036 PM 9447601 ER PT J AU Wolffe, AP AF Wolffe, AP TI When more is less SO NATURE GENETICS LA English DT Editorial Material ID GENE; DROSOPHILA C1 NICHD, Mol Embryol Lab, NIH, Bethesda, MD 20892 USA. RP Wolffe, AP (reprint author), NICHD, Mol Embryol Lab, NIH, Bethesda, MD 20892 USA. NR 11 TC 14 Z9 14 U1 0 U2 0 PU NATURE AMERICA INC PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD JAN PY 1998 VL 18 IS 1 BP 5 EP 6 DI 10.1038/ng0198-5 PG 2 WC Genetics & Heredity SC Genetics & Heredity GA YP265 UT WOS:000071259600003 PM 9425885 ER PT J AU Martinez-Mir, A Paloma, E Allikmets, R Ayuso, C del Rio, T Dean, M Vilageliu, L Gonzalez-Duarte, R Balcells, S AF Martinez-Mir, A Paloma, E Allikmets, R Ayuso, C del Rio, T Dean, M Vilageliu, L Gonzalez-Duarte, R Balcells, S TI Retinitis pigmentosa caused by a homozygous mutation in the Stargardt disease gene ABCR SO NATURE GENETICS LA English DT Letter C1 Univ Barcelona, Fac Biol, Dept Genet, E-08071 Barcelona, Spain. NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. Fdn Jimenez Diaz, Dept Genet, E-28040 Madrid, Spain. NCI, Lab Gen Divers, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. RP Gonzalez-Duarte, R (reprint author), Univ Barcelona, Fac Biol, Dept Genet, E-08071 Barcelona, Spain. RI Dean, Michael/G-8172-2012; IBIS, NEUROGENETICA/P-2947-2015; Balcells, Susana/C-5222-2017; OI Dean, Michael/0000-0003-2234-0631; Balcells, Susana/0000-0003-1211-1907; Ayuso, Carmen/0000-0002-9242-7065 NR 15 TC 278 Z9 283 U1 0 U2 8 PU NATURE AMERICA INC PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD JAN PY 1998 VL 18 IS 1 BP 11 EP 12 DI 10.1038/ng0198-11 PG 2 WC Genetics & Heredity SC Genetics & Heredity GA YP265 UT WOS:000071259600009 PM 9425888 ER PT J AU Southard-Smith, EM Kos, L Pavan, WJ AF Southard-Smith, EM Kos, L Pavan, WJ TI Sox10 mutation disrupts neural crest development in DOM Hirschsprung mouse model SO NATURE GENETICS LA English DT Article ID MICE LACKING GDNF; AUTOSOMAL SEX REVERSAL; ENDOTHELIN-B RECEPTOR; SRY-RELATED GENE; ENTERIC NEURONS; MESSENGER-RNA; CAMPOMELIC DYSPLASIA; MEGACOLON; MELANOCYTES; FAMILY AB Hirschsprung disease (HSCR, MIM #142623) is a multigenic neurocristopathy (neural crest disorder) characterized by absence of enteric ganglia in a variable portion of the distal colon. Subsets of HSCR individuals also present with neural crest-derived melanocyte deficiencies (Hirschsprung-Waardenburg, HSCR-WS, MIM #277580). Murine models have been instrumental in the identification and analysis of HSCR disease genes. These include mice with deficiencies of endothelin B receptor (Ednrb(s-l); refs 1,2) endothelin 3 (Edn3(ls); refs 1,3) the tyrosine kinase receptor cRet(4) and glial-derived neurotrophic factor(5-7). Another mouse model of HSCR disease, Dom, arose spontaneously at the Jackson Laboratory(8). While Dom/+ heterozygous mice display regional deficiencies of neural crest-derived enteric ganglia in the distal colon, Dom/Dom homozygous animals are embryonic lethal(8). We have determined that premature termination of Sox10, a member of the SRY-like HMG box family of transcription factors, is responsible for absence of the neural crest derivatives in Dom mice. We demonstrate expression of Sox10 in normal neural crest cells, disrupted expression of both Sox10 and the HSCR disease gene Ednrb in Dom mutant embryos, and loss of neural crest derivatives due to apoptosis. Our studies suggest that Sox10 is essential for proper peripheral nervous system development. We propose SOX10 as a candidate disease gene for individuals with HSCR whose disease does not have an identified genetic origin. C1 Natl Human Genome Res Inst, Mouse Embryol Sect, Lab Genet Dis Res, NIH, Bethesda, MD 20892 USA. RP Pavan, WJ (reprint author), Natl Human Genome Res Inst, Mouse Embryol Sect, Lab Genet Dis Res, NIH, Bldg 49,Room 4A82,49 Convent Dr,MSC 4472, Bethesda, MD 20892 USA. RI Southard-Smith, Michelle/C-3488-2012 NR 36 TC 490 Z9 499 U1 2 U2 12 PU NATURE AMERICA INC PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD JAN PY 1998 VL 18 IS 1 BP 60 EP 64 DI 10.1038/ng0198-60 PG 5 WC Genetics & Heredity SC Genetics & Heredity GA YP265 UT WOS:000071259600023 PM 9425902 ER PT J AU Xiao, S Nalabolu, SR Aster, JC Ma, JL Abruzzo, L Jaffe, ES Stone, R Weissman, SM Hudson, TJ Fletcher, JA AF Xiao, S Nalabolu, SR Aster, JC Ma, JL Abruzzo, L Jaffe, ES Stone, R Weissman, SM Hudson, TJ Fletcher, JA TI FGFR1 is fused with a novel zinc-finger gene, ZNF198, in the t(8;13) leukaemia/lymphoma syndrome SO NATURE GENETICS LA English DT Article ID FIBROBLAST GROWTH-FACTOR; T-CELL; CHROMOSOMAL TRANSLOCATIONS; LYMPHOBLASTIC LYMPHOMA; RECEPTOR; EOSINOPHILIA; ABERRATIONS; MALIGNANCY; ACTIVATION; MUTATIONS AB Various histological subtypes of leukaemia and lymphoma are associated with diagnostic chromosome translocations(1-4), and substantial strides have been made in determining the specific oncogenes targetted by those translocations. We report the cloning of a novel fusion oncogene associated with a unique leukaemia/lymphoma syndrome. Patients afflicted with this syndrome present with lymphoblastic lymphoma and a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow, which generally progress to full-blown acute myelogenous leukaemia within a year of diagnosis(5-9). A specific chromosome translocation, t(8;13)(p11;q11-12), is found in both lymphoma and myeloid leukaemia cells from these patients, supporting bi-lineage differentiation from a transformed stem cell(6-8). We find that the 8p11 translocation breakpoints, in each of four patients, interrupt intron 8 of the fibroblast growth factor receptor 1 gene (FGFR1). These translocations are associated with aberrant transcripts in which four predicted zinc-finger domains, contributed by a novel and widely expressed chromosome-13 gene (ZNF198), are fused to the FGFR1 tyrosine-kinase domain. Transient expression studies show that the ZNF198-FGFR1 fusion transcript directs the synthesis of an approximately 87-kD polypeptide, localizing predominantly to the cytoplasm. Our studies demonstrate an FGFR1 oncogenic role and suggest a tumorigenic mechanism in which ZNF198-FGFR1 activation results from ZNF198 zinc-finger-mediated homodimerization. C1 Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA. Yale Univ, Sch Med, New Haven, CT 06536 USA. MIT, Whitehead Inst, Ctr Genome Res, Cambridge, MA 02142 USA. Univ Maryland, Med Ctr, Dept Pathol, Baltimore, MD 21201 USA. NCI, Hematopathol Sect, Bethesda, MD 20892 USA. Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA. Childrens Hosp, Div Hematol Oncol, Boston, MA 02115 USA. RP Xiao, S (reprint author), Brigham & Womens Hosp, Dept Pathol, 75 Francis St, Boston, MA 02115 USA. FU NCI NIH HHS [CA72791] NR 30 TC 231 Z9 240 U1 0 U2 3 PU NATURE AMERICA INC PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD JAN PY 1998 VL 18 IS 1 BP 84 EP 87 DI 10.1038/ng0198-84 PG 4 WC Genetics & Heredity SC Genetics & Heredity GA YP265 UT WOS:000071259600029 PM 9425908 ER PT J AU Biesecker, L AF Biesecker, L TI Strike three for GLI3 (vol 17, pg 259, 1997) SO NATURE GENETICS LA English DT Correction C1 Natl Human Genome Res Inst, NIH, Lab Genet Dis Res, Bethesda, MD 20892 USA. RP Biesecker, L (reprint author), Natl Human Genome Res Inst, NIH, Lab Genet Dis Res, Bethesda, MD 20892 USA. NR 1 TC 3 Z9 3 U1 0 U2 1 PU NATURE AMERICA INC PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD JAN PY 1998 VL 18 IS 1 BP 88 EP 88 PG 1 WC Genetics & Heredity SC Genetics & Heredity GA YP265 UT WOS:000071259600030 ER PT J AU Preusch, PC Norvell, JC Cassatt, JC Cassman, M AF Preusch, PC Norvell, JC Cassatt, JC Cassman, M TI Progress away from 'no crystals, no grant' SO NATURE STRUCTURAL BIOLOGY LA English DT Editorial Material ID PHOTOSYNTHETIC REACTION-CENTER; CYTOCHROME-C-OXIDASE; LIGHT-HARVESTING COMPLEX; RHODOPSEUDOMONAS-VIRIDIS; RHODOBACTER-SPHAEROIDES; ANGSTROM RESOLUTION; SUGAR TRANSLOCATION; STRUCTURAL BASIS; 2.8 ANGSTROM; ELECTRON C1 NIGMS, Div Pharmacol Physiol & Biol Chem, NIH, Bethesda, MD 20892 USA. NIGMS, Div Cell Biol & Biophys, NIH, Bethesda, MD 20892 USA. RP Preusch, PC (reprint author), NIGMS, Div Pharmacol Physiol & Biol Chem, NIH, Bethesda, MD 20892 USA. EM preuschp@nigms.nih.gov NR 36 TC 16 Z9 17 U1 2 U2 2 PU NATURE AMERICA INC PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1072-8368 J9 NAT STRUCT BIOL JI Nat. Struct. Biol. PD JAN PY 1998 VL 5 IS 1 BP 12 EP 14 DI 10.1038/nsb0198-12 PG 3 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA YQ141 UT WOS:000071352600007 PM 9437421 ER PT J AU Adler, CM Malhotra, AK Goldberg, T Pickar, D Breier, A AF Adler, CM Malhotra, AK Goldberg, T Pickar, D Breier, A TI Effects of the NMDA antagonist, ketamine on formal thought disorder and on memory in healthy volunteers SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIMH, Expt Therapeut Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P35196 BP R77 EP R77 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700271 ER PT J AU Berglund, BA Fleming, PR Rice, K Howlett, AC AF Berglund, BA Fleming, PR Rice, K Howlett, AC TI Characterization of structural requirements for ligand binding to the CB1 and CB2 cannabinoid receptors. SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 St Louis Univ, Sch Med, Dept Pharmacol & Physiol Sci, St Louis, MO 63104 USA. NIDDK, Med Chem Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 2 MA P1744 BP R726 EP R726 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KW UT WOS:000075207801345 ER PT J AU Catt, KJ Hunyady, L AF Catt, KJ Hunyady, L TI Molecular determinants of angiotensin AT(1) receptor activation. SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIH, Endocrinol & Reprod Res Branch, Bethesda, MD 20892 USA. Semmelweis Univ Med, Dept Physiol, H-1085 Budapest, Hungary. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 2 MA W123 BP R579 EP R579 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KW UT WOS:000075207800785 ER PT J AU Chuang, DM Chen, RW Saunders, PA Ishitani, R AF Chuang, DM Chen, RW Saunders, PA Ishitani, R TI Roles of GAPDH, P53 and bax in neuronal apoptosis SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIMH, Mol Neurobiol Sect, Biol Psychiat Branch, NIH, Bethesda, MD 20892 USA. Josai Univ, Grp Cellular Neurobiol, Sakado, Saitama 35002, Japan. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 2 MA P5410 BP R431 EP R431 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KW UT WOS:000075207800231 ER PT J AU Clifford, JJ Tighe, O Croke, DT Drago, J Sibley, DR Waddington, JL AF Clifford, JJ Tighe, O Croke, DT Drago, J Sibley, DR Waddington, JL TI Targeted gene deletion of the D-1A dopamine receptor: Behavioural topography to D-1-like and D-2-like agonists SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 Royal Coll Surg Ireland, Dept Clin Pharmacol, Dublin 2, Ireland. Royal Coll Surg Ireland, Dept Biochem, Dublin 2, Ireland. Monash Univ, Clayton, Vic 3168, Australia. NINDS, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P77 BP R96 EP R96 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700343 ER PT J AU Eisenhofer, G Mezey, E Hiremagalur, B Pacak, K Friberg, P AF Eisenhofer, G Mezey, E Hiremagalur, B Pacak, K Friberg, P TI Contribution of adrenals to circulating catecholamines and catecholamine metabolites in humans SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NINDS, Clin Neurosci Branch, NIH, Bethesda, MD 20982 USA. NINDS, Basic Neurosci Program, NIH, Bethesda, MD 20982 USA. Sahlgrenska Hosp, Dept Clin Physiol, Goteborg, Sweden. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 2 MA P677 BP R610 EP R610 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KW UT WOS:000075207800898 ER PT J AU Gonzalez, FJ AF Gonzalez, FJ TI Unraveling the function of P450s and xenobiotic receptors SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NCI, Lab Metab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA L5 BP R6 EP R6 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700007 ER PT J AU Gottesman, MM Hrycyna, CA Hafkemeyer, P Dey, S Zhou, Y Shoshani, T Pastan, I AF Gottesman, MM Hrycyna, CA Hafkemeyer, P Dey, S Zhou, Y Shoshani, T Pastan, I TI Molecular dissection of the multidrug transporter SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NCI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. NCI, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA SB21 BP R24 EP R24 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700067 ER PT J AU Johren, O Sanvitto, GL Saavedra, JM AF Johren, O Sanvitto, GL Saavedra, JM TI Dopaminergic neurons in the female arcuate nucleus: Sex-specific, estrogen-progesterone induced expression of angiotensin II AT(1A) receptor mRNA SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIMH, Pharmacol Sect, NIH, Bethesda, MD 20892 USA. RI Johren, Olaf/G-6967-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P3555 BP R41 EP R41 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700131 ER PT J AU Leitner, B Lovisetti-Scamihorn, P Heilmann, J Eiden, L Winkler, H AF Leitner, B Lovisetti-Scamihorn, P Heilmann, J Eiden, L Winkler, H TI Membrane associated proteins of vesicles in an adrenergic nerve SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 Univ Innsbruck, Dept Pharmacol, A-6020 Innsbruck, Austria. NIH, Cell Biol Lab, Bethesda, MD 20205 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 2 MA P311 BP R411 EP R411 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KW UT WOS:000075207800154 ER PT J AU Mitsuhata, C Kitayama, S Satoh, T Morita, K Vandenbergh, D Uhl, GR Dohi, T AF Mitsuhata, C Kitayama, S Satoh, T Morita, K Vandenbergh, D Uhl, GR Dohi, T TI Involvement of tyrosine-533 of rat dopamine transporter in interaction with 1-methyl-4-phenylpyridinium (MPP+) and cocaine SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 Hiroshima Univ, Sch Dent, Dept Pharmacol, Minami Ku, Hiroshima 7348553, Japan. NIDA, Mol Neurol Branch, Intramural Res Program, NIH, Baltimore, MD 21224 USA. Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21224 USA. Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P76 BP R96 EP R96 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700342 ER PT J AU Murphy, PM AF Murphy, PM TI Classification and nomenclature of chemokine receptors SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIAID, Host Def Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 2 MA SA54 BP R581 EP R581 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KW UT WOS:000075207800793 ER PT J AU Paruszewski, R Rostafinska, G Strupinska, M Stables, JP AF Paruszewski, R Rostafinska, G Strupinska, M Stables, JP TI Amino acidic excitatory amino acid receptor antagonists are required to be amides with a moderate hydrophobicity SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 Med Univ, Dept Drug Chem, PL-02097 Warszawa, Poland. NINDS, Div Convuls Dev & Neuromuscular Disorders, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P112 BP R133 EP R133 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700486 ER PT J AU Putney, JW McKay, RR Huang, Y Bird, GS AF Putney, JW McKay, RR Huang, Y Bird, GS TI Capacitative calcium entry SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 2 MA SF63 BP R378 EP R378 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KW UT WOS:000075207800034 ER PT J AU Rauhala, P Lin, AMY Chiueh, CC AF Rauhala, P Lin, AMY Chiueh, CC TI Neuroprotection by S-nitrosoglutathione (GSNO) of brain dopamine neurons from iron-induced oxidative stress in vivo SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 Vet Gen Hosp, Dept Res & Educ, Taipei, Taiwan. NIMH, Unit Neurodegeneration & Neuroprotect, Clin Sci Lab, NIH Clin Ctr 103D41, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P1822 BP R301 EP R301 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207701121 ER PT J AU Rauhala, P Khaldi, A Mohanakumar, KP Chiueh, CC AF Rauhala, P Khaldi, A Mohanakumar, KP Chiueh, CC TI Hydroxyl radicals rather than nitric oxide mediate brain injury and lipid peroxidation induced by sodium nitroprusside SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIMH, India Inst Chem Biol, Unit Neurodegenerat & Neuroprotect, Lab Clin Sci,NIH Clin Ctr, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P1821 BP R301 EP R301 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207701120 ER PT J AU Rauhala, P Lin, AMY Chiueh, CC AF Rauhala, P Lin, AMY Chiueh, CC TI Neuroprotection by S-nitrosoglutathione (GSNO) of brain dopamine neurons from iron-induced oxidative stress in vivo SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIMH, Clin Sci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA W82 BP R206 EP R206 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700748 ER PT J AU Schetz, JA Sibley, DR AF Schetz, JA Sibley, DR TI Zinc modulates antagonist interactions with D-2-like dopamine receptors by distinct molecular mechanisms SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NINDS, Mol Neuropharmacol Sect, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P79 BP R97 EP R97 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700345 ER PT J AU Sibley, DR Hollon, TR Grinberg, A Huang, SP Drago, J Westphal, H AF Sibley, DR Hollon, TR Grinberg, A Huang, SP Drago, J Westphal, H TI Progress in the creation of a D-5 dopamine receptor knock-out mouse SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NINDS, Mol Neuropharmacol Sect, NIH, Bethesda, MD 20892 USA. NR 0 TC 2 Z9 2 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 2 MA SC42 BP R375 EP R375 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KW UT WOS:000075207800024 ER PT J AU Sora, I Ii, XF Zeng, Z Kinsey, S Kitanaka, N Kitanaka, J Goodman, N Elmer, G Uhl, GR AF Sora, I Ii, XF Zeng, Z Kinsey, S Kitanaka, N Kitanaka, J Goodman, N Elmer, G Uhl, GR TI Mu opiate receptor knock-out mice: Influence on pain, locomotion and reward models SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21224 USA. Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21224 USA. NIDA, IRP, NIH, Baltimore, MD 21224 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P1339 BP R347 EP R347 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207701303 ER PT J AU Sora, I Hall, S Wang, ZJ Fuchs, P Li, XF Kitanaka, N Zeng, ZZ Raja, SN Uhl, GR AF Sora, I Hall, S Wang, ZJ Fuchs, P Li, XF Kitanaka, N Zeng, ZZ Raja, SN Uhl, GR TI Mu opiate receptor genes, nociception and reward: Potential lessons in knockout mice, mouse strains and humans. SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 JHUSM, Baltimore, MD USA. NIDA, IRP, NIH, Baltimore, MD USA. NIAAA, Clin Studies Lab, NIH, Rockville, MD 20852 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P1340 BP R347 EP R347 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207701304 ER PT J AU Southan, GJ Salzman, AL Szabo, C AF Southan, GJ Salzman, AL Szabo, C TI Hydroxyguanidines as inhibitors of the oxidation and cytotoxicity induced by peroxynitrite SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. Childrens Hosp, Med Ctr, Div Crit Care, Cincinnati, OH 45229 USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P1812 BP R298 EP R298 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207701111 ER PT J AU van Bergen, P Rauhala, P Chiueh, CC AF van Bergen, P Rauhala, P Chiueh, CC TI Different effects of hemoglobin and its metabolites on brain lipid peroxidation SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIMH, Unit Neurotoxicol & Neuroprotect, Clin Sci Lab, NIH,Clin Ctr, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P710 BP R97 EP R97 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700346 ER PT J AU Wess, J AF Wess, J TI G protein-coupled receptors: Molecular mechanisms governing receptor assembly and G protein recognition SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIDDKD, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA SD13 BP R192 EP R192 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700705 ER PT J AU Yu, S Weinstein, LS AF Yu, S Weinstein, LS TI Gs alpha knockout mice demonstrate that the Gs alpha gene is imprinted in a tissue-specific manner SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIDDK, Metab Dis Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 2 MA SD21 BP R384 EP R384 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KW UT WOS:000075207800055 ER PT J AU Ziganshin, AU Rychkov, AV Ziganshina, LE Kim, YC Camaioni, E Burnstock, G Jacobson, KA AF Ziganshin, AU Rychkov, AV Ziganshina, LE Kim, YC Camaioni, E Burnstock, G Jacobson, KA TI Antagonistic profiles of new derivatives of pyridoxalphosphate-6-azophenyl-2 ',4 '-disulphonic acid SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 Kazan Med Univ, Dept Pharmacol, Kazan 420012, Russia. NIDDKD, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. Royal Free Sch Med, Autonom Neurosci Inst, London NW3 2PF, England. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P1044 BP R132 EP R132 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700484 ER PT J AU Zimmer, A Zimmer, AM Konig, M Brady, L Herkenham, M Mezey, E Palkovits, M AF Zimmer, A Zimmer, AM Konig, M Brady, L Herkenham, M Mezey, E Palkovits, M TI Behavioral studies in preproenkephalin and substance P knockout mice SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIMH, Bethesda, MD 20892 USA. RI Palkovits, Miklos/F-2707-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA W46 BP R199 EP R199 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700728 ER PT J AU Zimmer, A Steiner, H Zimmer, AM Herkenham, M Hohman, A Kitai, ST Buckley, N Mezey, E Bonner, T AF Zimmer, A Steiner, H Zimmer, AM Herkenham, M Hohman, A Kitai, ST Buckley, N Mezey, E Bonner, T TI Pharmacological and behavioral studies of cannabinoid receptor knockout mice SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 NIMH, Bethesda, MD 20892 USA. NINDS, Bethesda, MD 20892 USA. Univ Tennessee, Memphis, TN 38163 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 358 IS 1 SU 1 MA P35213 BP R81 EP R81 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 107KV UT WOS:000075207700288 ER PT J AU Buters, JTM Gonzalez, FJ Greim, H Jefcoate, CR Luch, A Soballa, V Seidel, A Doehmer, J AF Buters, JTM Gonzalez, FJ Greim, H Jefcoate, CR Luch, A Soballa, V Seidel, A Doehmer, J TI Knockout mice to study the role of CYP1B1 in carcinogenesis in vivo SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 TU Munchen, Inst Toxikiol & Umwelthyg, D-80636 Munchen, Germany. Univ Wisconsin, Sch Med, Dept Pharmacol, Madison, WI 53706 USA. NIH, Mol Carcinogenesis Lab, Bethesda, MD 20892 USA. Univ Mainz, Inst Toxikol, D-55131 Mainz, Germany. GSF, Forschungszentrum, Inst Toxikol, D-85764 Neuherberg, Germany. RI Buters, Jeroen/G-5070-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 357 IS 4 SU S MA 513 BP R132 EP R132 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA ZM863 UT WOS:000073583900521 ER PT J AU Ochs, K Sobol, RW Wilson, S Kaina, B AF Ochs, K Sobol, RW Wilson, S Kaina, B TI Mammalian cells deficient in DNA polymerase beta are hypersensitive to alkylating agent-induced cytotoxicity, apoptosis and chromosomal breakage SO NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY LA English DT Meeting Abstract C1 Univ Mainz, Inst Toxicol, Div Appl Toxicol, D-55131 Mainz, Germany. NIEHS, Struct Biol Lab, Res Triangle Pk, NC 27709 USA. RI Sobol, Robert/E-4125-2013 OI Sobol, Robert/0000-0001-7385-3563 NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0028-1298 J9 N-S ARCH PHARMACOL JI Naunyn-Schmiedebergs Arch. Pharmacol. PY 1998 VL 357 IS 4 SU S MA 574 BP R147 EP R147 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA ZM863 UT WOS:000073583900582 ER PT B AU Rapoport, S AF Rapoport, S BE Folstein, MF TI Brain imaging in Alzheimer's disease SO NEUROBIOLOGY OF PRIMARY DEMENTIA LA English DT Proceedings Paper CT Conference on Primary Dementia CY DEC 02-03, 1994 CL TUFTS UNIV, BOSTON, MA SP Assoc Res Nervous & Mental Dis HO TUFTS UNIV C1 NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. RP Rapoport, S (reprint author), NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER PSYCHIATRIC PRESS, INC PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 USA BN 0-88048-915-4 PY 1998 BP 235 EP 270 PG 36 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA BL67J UT WOS:000076281600012 ER PT S AU Hope, BT AF Hope, BT BE Ali, SF TI Cocaine and the AP-1 transcription factor complex SO NEUROCHEMISTRY OF DRUGS OF ABUSE: COCAINE, IBOGAINE, AND SUBSTITUTED AMPHETAMINES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Meeting on Cellular and Molecular Mechanisms of Drugs of Abuse - Cocaine, Ibogaine, and Substituted Amphetamines CY JUL 16-18, 1997 CL HAMILTON, BERMUDA SP Int Soc Neurochem, Amer Soc Neurochem, Res Biochem Int, Servier Amerique, Warner Lambert Co, Parke Davis, Neurosci Res Div, US FDA, Natl Ctr Toxicol Res ID MOLECULAR MECHANISMS; ELECTROCONVULSIVE SEIZURE; NUCLEUS-ACCUMBENS; MESSENGER-RNAS; DELTA-FOSB; GENE; PROTEINS; STRIATUM; EXPRESSION; ADDICTION AB Cocaine addiction in humans develops gradually with repeated administrations and persists long after cocaine has cleared the body The mechanisms underlying this persistent form of neuroplasticity are not understood and can involve both structural and biochemical mechanisms, The long time course for cocaine addiction in humans and for development of cocaine self-administration in animal models suggests the involvement of alterations in gene expression leading to altered signaling in the brain. In the striatum (Str) and nucleus accumbens (NAc) of rats, pretreatment with repeated cocaine administrations downregulates the induction of various immediate early genes (IEGs) by a subsequent acute challenge with cocaine. Some of these downregulated IEGs encode Fos-related components of the activator protein-1 (AP-I) complex, which is likely to regulate a number of genes important for neuronal function, Interestingly, repeated cocaine administration induces novel delta FosB-related proteins (called chronic Foe-related antigens (Fras)) in the NAc and Str that replace the downregulated isoforms of Fos, Unlike the acutely induced, short-lasting isoforms of Fos and FosB, the chronic Fras persist long after the last cocaine administration. The known form of delta FosB per se lacks the domain required to activate transcription. If the chronic Fras are similar in structure to delta FosB, then the induction of chronic Fras likely leads to a blockade of AP-l-dependent transcription resulting in altered gene expression. We are presently purifying the chronic Fras to obtain amino acid sequence in order to directly examine our hypothesis about the effects of repeated cocaine administration on AP-l-dependent transcription and gene expression in the brain. C1 NINDS, Mol Plastic Sect, NIH, Bethesda, MD 20892 USA. RP Hope, BT (reprint author), NINDS, Mol Plastic Sect, NIH, Bldg 36,Room 4C23,36 Convent Dr,MSC-4135, Bethesda, MD 20892 USA. EM hope@codon.nih.gov RI Hope, Bruce/A-9223-2010 OI Hope, Bruce/0000-0001-5804-7061 NR 29 TC 21 Z9 22 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-145-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 844 BP 1 EP 6 DI 10.1111/j.1749-6632.1998.tb08216.x PG 6 WC Substance Abuse; Multidisciplinary Sciences; Neurosciences SC Substance Abuse; Science & Technology - Other Topics; Neurosciences & Neurology GA BL26D UT WOS:000074929000001 PM 9668659 ER PT S AU Rothman, RB Elmer, GI Shippenberg, TS Rea, W Baumann, MH AF Rothman, RB Elmer, GI Shippenberg, TS Rea, W Baumann, MH BE Ali, SF TI Phentermine and fenfluramine - Preclinical studies in animal models of cocaine addiction SO NEUROCHEMISTRY OF DRUGS OF ABUSE: COCAINE, IBOGAINE, AND SUBSTITUTED AMPHETAMINES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Meeting on Cellular and Molecular Mechanisms of Drugs of Abuse - Cocaine, Ibogaine, and Substituted Amphetamines CY JUL 16-18, 1997 CL HAMILTON, BERMUDA SP Int Soc Neurochem, Amer Soc Neurochem, Res Biochem Int, Servier Amerique, Warner Lambert Co, Parke Davis, Neurosci Res Div, US FDA, Natl Ctr Toxicol Res ID OBSESSIVE-COMPULSIVE DISORDER; RECEPTOR AGONISTS; NUCLEUS-ACCUMBENS; SUICIDAL-BEHAVIOR; DOPAMINE; SEROTONIN; RATS; WITHDRAWAL; SENSITIZATION; COMBINATION AB Combined dopamine (DA) and 5-hydroxytryptamine (5-HT) releasers such as phentermine (PHEN) and fenfluramine (FEN) are reported, in open label studies, to reduce craving for alcohol and cocaine and to prevent relapse. The objective of the studies reported here was to assess the actions of these agents alone and in combination in various animal models of drug addiction. Study 1, In Fire microdialysis experiments demonstrate that these agents preferentially release mesolimbic DA (PHEN) and 5-HT (FEN). Patients who relapse and use cocaine while taking these medications report diminished cocaine-like subjective effects. Microdialysis experiments were performed in awake rats, and dialysate samples were analyzed for DA and 5-HT. PHEN (1 mg/kg intravenously (i.v.)) elevated DA (2-3-fold) for over 1.5 hr. Administration of cocaine (3 mg/kg, i.v.) increased DA 6-fold in saline-treated rats, but only 3-fold in PHEN-treated rats. PHEN did not reduce cocaine-induced increases in 5-HT. Study 2. These agents were assessed in a mouse model of cocaine-conditioned motoric activity (CCMA). Pretreatment with nonactivating doses of PHEN (4.6 mg/kg, intraperitoneally (i.p.)) enhanced CCMA, whereas non-depressing doses of FEN (0.1 mg/kg, i.p.) did not alter CCMA or the PHEN-induced increase in CCMA. In contrast, sub-effective doses of FEN reduced CCMA stereotypy-like locomotion, whereas sub-effective doses of PHEN were without effect. PHEN reversed the FEN-induced increase in CCMA stereotypy-like locomotion, Study 3, PHEN and FEN were assessed in the conditioned place preference model. FEN produced marked aversions for an environment previously associated with its administration and the minimum dose producing this effect was 3.0 mg/kg, In contrast, administration of PHEN, amphetamine (1.0-3.0 mg/kg) or morphine (3.1-5.0 mg/kg) produced dose-related preferences for the drug-paired place. However, the magnitude of the response to PHEN was less than that produced by the other prototypic drugs of abuse, In rats that received FEN (0.3 or 3.0 mg/kg) in combination with PHEN (3.0 mg/kg), the conditioned rewarding effects of PHEN were abolished. These data demonstrate that the rewarding effects of PHEN can be conditioned to stimuli previously associated with its administration. However, the conditioned response to this agent is less then that produced by prototypic drugs of abuse. The finding that PHEN-induced place preferences were attenuated by doses of FEN demonstrates that the combination of FEN/PHEN is devoid of motivational effects. The preclinical data obtained with PHEN/FEN in various models of drug provide a strong rationale for pursuing controlled clinical trials in humans with agents that act via a similar mechanism of action. C1 NIDA, DIR, Clin Psychopharmacol Sect, NIH, Baltimore, MD 21224 USA. NIDA, DIR, Integrat Neurosci Unit, NIH, Baltimore, MD 21224 USA. NIDA, DIR, Brain Imaging Sect, NIH, Baltimore, MD 21224 USA. Univ Maryland, Maryland Psychiat Res Ctr, Dept Psychiat, Sch Med, Catonsville, MD 21228 USA. RP Rothman, RB (reprint author), NIDA, DIR, Clin Psychopharmacol Sect, NIH, 550 Nathan Shock Dr, Baltimore, MD 21224 USA. EM rrothman@irp.nida.nih.gov NR 40 TC 39 Z9 39 U1 2 U2 4 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-145-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 844 BP 59 EP 74 DI 10.1111/j.1749-6632.1998.tb08222.x PG 16 WC Substance Abuse; Multidisciplinary Sciences; Neurosciences SC Substance Abuse; Science & Technology - Other Topics; Neurosciences & Neurology GA BL26D UT WOS:000074929000007 PM 9668665 ER PT S AU Jayanthi, S Ladenheim, B Cadet, JL AF Jayanthi, S Ladenheim, B Cadet, JL BE Ali, SF TI Methamphetamine-induced changes in antioxidant enzymes and lipid peroxidation in copper/zinc-superoxide dismutase transgenic mice SO NEUROCHEMISTRY OF DRUGS OF ABUSE: COCAINE, IBOGAINE, AND SUBSTITUTED AMPHETAMINES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Meeting on Cellular and Molecular Mechanisms of Drugs of Abuse - Cocaine, Ibogaine, and Substituted Amphetamines CY JUL 16-18, 1997 CL HAMILTON, BERMUDA SP Int Soc Neurochem, Amer Soc Neurochem, Res Biochem Int, Servier Amerique, Warner Lambert Co, Parke Davis, Neurosci Res Div, US FDA, Natl Ctr Toxicol Res ID NITRIC-OXIDE SYNTHASE; RAT-BRAIN; GLUTATHIONE-PEROXIDASE; UPTAKE SITES; INDUCED NEUROTOXICITY; HYDROGEN-PEROXIDE; DOPAMINE; NEURONS; ATTENUATION; CATALASE AB The present study was conducted to investigate the effects of methamphetamine (METH)-induced toxicity on brain cortical and striatal antioxidant defense systems. Because METH-induced toxicity is attenuated in copper/zinc-superoxide dismutase transgenic (Cu/Zn-SOD-Tg) mice, we sought to determine.if METH had differential effect on antioxidant enzymes on these mice in comparison to non-Tg mice. METH (4 x 10 mg/kg) induced a significant decrease in Cu/Zn-SOD activity in the cortical region without altering striatal enzymatic activity in non-Tg mice; whereas homozygous SOD-Tg mice showed a significant increase in the striatum. In addition, METH caused decrease in catalase (CAT) activity in the striatum of non-Tg mice and significant increase in the cortex of homozygous SOD-Tg mice. METH also induced decreases in glutathione peroxidase (GSH-Px) in both cortical and striatal regions of non-Tg mice and in the striatum of heterozygous SOD-TO mice. Lipid peroxidation was increased in both cortices and striata of non-Tg and heterozygous SOD-Tg mice, whereas the homozygous SOD-Tg mice were not affected. These results are discussed in term!, of their substantiation of a role for oxygen-based radicals in METH-induced toxicity hi rodents. C1 NIDA, Div Intramural Res, Mol Neuropsychiat Sect, NIH, Baltimore, MD 21224 USA. RP Cadet, JL (reprint author), NIDA, Div Intramural Res, Mol Neuropsychiat Sect, NIH, POB 5180, Baltimore, MD 21224 USA. EM jcadet@irp.nida.nih.gov NR 41 TC 75 Z9 76 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-145-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 844 BP 92 EP 102 DI 10.1111/j.1749-6632.1998.tb08224.x PG 11 WC Substance Abuse; Multidisciplinary Sciences; Neurosciences SC Substance Abuse; Science & Technology - Other Topics; Neurosciences & Neurology GA BL26D UT WOS:000074929000009 PM 9668667 ER PT S AU Baumann, MH Ayestas, MA Rothman, RB AF Baumann, MH Ayestas, MA Rothman, RB BE Ali, SF TI In vivo correlates of central serotonin function after high-dose fenfluramine administration SO NEUROCHEMISTRY OF DRUGS OF ABUSE: COCAINE, IBOGAINE, AND SUBSTITUTED AMPHETAMINES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Meeting on Cellular and Molecular Mechanisms of Drugs of Abuse - Cocaine, Ibogaine, and Substituted Amphetamines CY JUL 16-18, 1997 CL HAMILTON, BERMUDA SP Int Soc Neurochem, Amer Soc Neurochem, Res Biochem Int, Servier Amerique, Warner Lambert Co, Parke Davis, Neurosci Res Div, US FDA, Natl Ctr Toxicol Res ID BRAIN-SEROTONIN; RAT-BRAIN; 5-HYDROXYTRYPTAMINE RELEASE; EXTRACELLULAR SEROTONIN; PARA-CHLOROAMPHETAMINE; D-NORFENFLURAMINE; BEHAVIORAL EVIDENCE; NERVE-TERMINALS; INVIVO DIALYSIS; DORSAL RAPHE AB High doses of fenfluramine (FEN) are known to deplete central serotonin (5-HT) in animals, but functional impairments associated with such 5-HT depletion have been difficult to identify. In the present work, we examined neuroendocrine responsiveness in rats exposed to repeated high-dose FEN treatment, Male rats fitted with indwelling catheters received FEN (20 mg/kg, subcutaneously, twice a day) or saline for 4 days, At 1 and 2 weeks after treatment, rats were challenged with intravenous FEN (1.5 & 3 mg/kg) or saline. Repeated blood samples were drawn, and plasma was assayed for prolactin and corticosterone by radioimmunoassay, Acute FEN challenge caused dose-dependent elevations of plasma prolactin and corticosterone in all rats. However, the FEN-induced hormone responses were significantly blunted (p < 0.01) in rats previously exposed to FEN. The repeated FEN dosing regimen dramatically reduced (>50%) postmortem 5-HT levels in the mediobasal hypothalamus, basolateral amygdala, and hippocampus, while the lateral hypothalamus was unaffected, These data suggest th:it high-dose FEN causes alterations in central 5-HT systems involved with pituitary hormone secretion. The relevance of the present data to the clinical use of FEN is unclear. Because the neuroendocrine challenge paradigm is able to identify functional 5-HT deficits in rats, we propose that similar experiments should be performed in humans. Neuroendocrine challenge tests represent a reliable method to test the existence of FEN-induced neurotoxicity in human patients undergoing long-term FEN treatment. C1 NIDA, Div Intramural Res, Clin Psychopharmacol Sect, NIH, Baltimore, MD 21224 USA. RP Baumann, MH (reprint author), NIDA, Div Intramural Res, Clin Psychopharmacol Sect, NIH, POB 5180,5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM mbaumann@irp.nida.nih.gov NR 62 TC 5 Z9 5 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-145-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 844 BP 138 EP 152 DI 10.1111/j.1749-6632.1998.tb08229.x PG 15 WC Substance Abuse; Multidisciplinary Sciences; Neurosciences SC Substance Abuse; Science & Technology - Other Topics; Neurosciences & Neurology GA BL26D UT WOS:000074929000014 PM 9668672 ER PT S AU Baumann, MH Rothman, RB Ali, SF AF Baumann, MH Rothman, RB Ali, SF BE Ali, SF TI Neurochemical and neuroendocrine effects of ibogaine in rats: Comparison to MK-801 SO NEUROCHEMISTRY OF DRUGS OF ABUSE: COCAINE, IBOGAINE, AND SUBSTITUTED AMPHETAMINES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Meeting on Cellular and Molecular Mechanisms of Drugs of Abuse - Cocaine, Ibogaine, and Substituted Amphetamines CY JUL 16-18, 1997 CL HAMILTON, BERMUDA SP Int Soc Neurochem, Amer Soc Neurochem, Res Biochem Int, Servier Amerique, Warner Lambert Co, Parke Davis, Neurosci Res Div, US FDA, Natl Ctr Toxicol Res ID NMDA RECEPTOR COMPLEX; ANTI-ADDICTIVE DRUG; PARASAGITTAL ZONES; PRIMARY METABOLITE; IN-VIVO; MORPHINE; COCAINE; BINDING; CORTICOSTERONE; AMPHETAMINE AB Ibogaine (IBO) is a naturally-occurring indole compound that is being evaluated as a potential medication for substance use disorders. Although the precise mechanism of IBO action is unclear, recent in vitro data show this drug displays properties similar to the noncompetitive N-methyl-D-aspartate (NMDA) antagonist MK-801. The purpose of the present work was to compare in vivo neurobiological effects of IBO and MK-801 in rats. Groups of male rats (n = 6-8/group) were decapitated 30 and 60 min after receiving intraperitoneal (i.p.) IBO (10 & 100 mg/kg), MK-801 (0.1 & 1.0 mg/kg) or vehicle. Trunk blood was collected for the analysis of plasma prolactin and corticosterone; brains were harvested and dissected for determination of dopamine (DA), serotonin (5-HT) and their metabolites. Both IBO and MK-801 increased corticosterone secretion, but only IBO elevated plasma prolactin, IBO produced dramatic reductions in tissue DA levels with concurrent increases in the metabolites, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA). This profile of IBO-induced changes in DA transmission was observed in the striatum, olfactory tubercle, and hypothalamus. The effects of MK-801 on DA metabolism did not mimic IBO, as MK-801 tended to increase DA and its metabolites. Neither drug appreciably affected 5-HT systems. Our results suggest that the effects of IBO on neuroendocrine function and DA transmission are not due to MK-801-like properties of IBO. Thus, the in vivo mechanism of IBO action cannot be explained simply on the basis of antagonism at NMDA receptors. C1 NIDA, Clin Psychopharmacol Sect, Div Intramural Res, NIH, Baltimore, MD 21224 USA. US FDA, Natl Ctr Toxicol Res, Div Neurotoxicol, Neurochem Lab, Jefferson, AR 72079 USA. RP Baumann, MH (reprint author), NIDA, Clin Psychopharmacol Sect, Div Intramural Res, NIH, POB 5180,5500 Nathan Shock Dr, Baltimore, MD 21224 USA. NR 43 TC 4 Z9 4 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-145-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 844 BP 252 EP 264 DI 10.1111/j.1749-6632.1998.tb08240.x PG 13 WC Substance Abuse; Multidisciplinary Sciences; Neurosciences SC Substance Abuse; Science & Technology - Other Topics; Neurosciences & Neurology GA BL26D UT WOS:000074929000025 PM 9668683 ER PT S AU Sternberg, EM AF Sternberg, EM BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI Overview of the conference and the field - Introduction SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MARYLAND SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID PITUITARY-ADRENAL AXIS; CENTRAL-NERVOUS-SYSTEM; CORTICOTROPIN-RELEASING HORMONE; RECEPTOR MESSENGER-RNA; RAT-BRAIN; INTERLEUKIN-1 RECEPTOR; MOUSE-BRAIN; LEWIS RATS; NEURONS; CYTOKINES AB The field of neuroimmune interactions is a prime example of interdisciplinary research spanning immunology, neurobiology, neuroendocrinology, and behavioral sciences. It also exemplifies research from the molecular to the clinical domain. The greatest challenge of the field, which this conference seeks to stimulate, is research that is at the same time precise, focused, and integrative. Several levels of interdisciplinary overlap will be highlighted. At the molecular level, neuro-and immune mediator molecules or their receptors may be members of the same superfamily or may regulate each other's expression or function. Most extensively studied are cytokine-neuropeptide/neurotransmitter interactions, including expression of cytokines within the central nervous system and production of neuropeptides by immune cells or at inflammatory sites. Advances relating cytokine-neurohormone interactions to mechanisms of apoptosis will ultimately shed light on the role of neuroimmune interactions in neuronal cell death and survival and immune cell selection, processes important in neuronal plasticity and immune specificity. At a systems level, advances have been made in cross-disciplinary application of modes of thinking. Incorporation of neurobiology's appreciation of anatomical organization, endocrinology's temporal dimension of neurohormonal secretion, and immunology's understanding of stimulus specificity all contribute to a more precise definition of how these complex systems interact at multiple levels. More precise understanding of effects of disruptions of these communications on disease susceptibility and expression will clarify how perturbations of one system, such as stimulation of the neuroendocrine stress response, might affect expression of disease in the other, such as autoimmune/inflammatory or infectious diseases. C1 NIMH, NIH, Bethesda, MD 20892 USA. RP Sternberg, EM (reprint author), NIMH, NIH, Bldg 10,Rm 2D-46,10 Ctr Dr,MSC 1284, Bethesda, MD 20892 USA. NR 43 TC 10 Z9 10 U1 1 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 1 EP 8 DI 10.1111/j.1749-6632.1998.tb09543.x PG 8 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500001 PM 9629231 ER PT S AU Grimm, MC Ben-Baruch, A Taub, DD Howard, OMZ Wang, JM Oppenheim, JJ AF Grimm, MC Ben-Baruch, A Taub, DD Howard, OMZ Wang, JM Oppenheim, JJ BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI Opiate inhibition of chemokine-induced chemotaxis SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MARYLAND SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID RECEPTOR GENE-EXPRESSION; NITRIC-OXIDE; MORPHINE; HIV-1; CELLS; PROTEINS; INVERTEBRATE; GRANULOCYTES; IMMUNOCYTES; INFECTION AB Chemokines consist of a family of 8-16-kDa cytokines that are generated very early in a wide variety of inflammatory responses and attract leukocytes to local sites. At nanomolar concentrations chemokines initiate signal transduction and activate leukocytes through seven transmembrane receptors (STM), but higher micromolar doses result in homologous desensitizing effects. On the basis of reports that opiates have anti-inflammatory effects and also use STM, we have investigated the possibility that they may cross-desensitize the response of leukocytes to chemokines. We have confirmed previous observations that met-enkephalin (MET) is chemotactic for human peripheral blood monocytes. Furthermore, we observed that preincubation of monocytes or neutrophils with MET or morphine prevented their subsequent chemotaxis in response to chemokines (MIP1 alpha or IL-8). However, MET did not inhibit the chemotactic response of PMN to NAP-2 a homologous chemokine that is less potent than IL-8 but cannot be desensitized. The inhibitory effect of opiates on chemokine-induced chemotaxis was antagonized by naloxone. Since MIP-1 alpha and IL-8, unlike NAP-2, have the capacity to desensitize leukocytes, it is possible that opiates, by desensitizing some chemokine responses, can suppress inflammatory reactions. C1 NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, Intramural Res Support Program,SAIC Frederick, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, Mol Immunoregulat Lab, Div Basic Sci, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, Clin Serv Program, Frederick, MD 21702 USA. RP Grimm, MC (reprint author), NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, Intramural Res Support Program,SAIC Frederick, Bldg 560,Rm 21-89A, Frederick, MD 21702 USA. RI Howard, O M Zack/B-6117-2012 OI Howard, O M Zack/0000-0002-0505-7052 NR 32 TC 61 Z9 63 U1 0 U2 4 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 9 EP 20 DI 10.1111/j.1749-6632.1998.tb09544.x PG 12 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500002 PM 9629232 ER PT S AU Webster, EL Torpy, DJ Elenkov, IJ Chrousos, GP AF Webster, EL Torpy, DJ Elenkov, IJ Chrousos, GP BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI Corticotropin-releasing hormone and inflammation SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MARYLAND SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID BLOOD MONONUCLEAR-CELLS; FACTOR-RECEPTOR SUBTYPE; RAT-BRAIN; IN-VITRO; FUNCTIONAL EXPRESSION; NERVOUS-SYSTEM; MOUSE SPLEEN; FACTOR CRF; IDENTIFICATION; SECRETION AB Corticotropin-releasing hormone (CRH) is a major regulator of the hypothalamic-pituitary-adrenal axis (HPA) and principal coordinator of the stress response. As in stress, intracerebroventricular administration of CRH suppresses the immune system indirectly, via glucocorticoid and/or sympathetic system-mediated mechanisms. Also, during inflammatory stress, the cytokines TNF alpha IL-1, and IL-6 stimulate hypothalamic CRH and/or vasopressin secretion as a way of preventing the inflammatory reaction from overreacting. Recently, CRH receptors were described in peripheral sites of the immune system, and CRH was found to promote several immune functions in vitro. We demonstrated a direct role of CRH in the inflammatory immune process in vivo, by first studying the effect of systemic CRH immunoneutralization in an experimental model of carrageenin-induced aseptic inflammation in Sprague-Dawley rats. We extended these observations to other forms of experimental inflammation, including streptococcal cell wall polysaccharide-and adjuvant-induced arthritides and peptide R16 (epitope of the interphotoreceptor retinoid-binding protein)-induced uveitis in Lewis rats. We also studied human disease states, including rheumatoid arthritis, Hashimoto thyroiditis, and ulcerative colitis. Inflamed tissues contained large amounts of IR CRH, reaching levels similar to those observed in the hypophyseal portal system. We also demonstrated the presence of CRH mRNA and CRH receptors in inflammatory cells and identified the mast cells as a major immune target for CRH. In addition to production by immune cells, the peripheral nervous system, including the postganglionic sympathetic neurons and the sensory fibers type C, appears to contribute to IR CRH production in inflammatory sites. The production of CRH from the postganglionic sympathetic neurons may be responsible for the stress-induced activation of allergic/autoimmune phenomena, such as asthma and eczema, via mast cell degranulation. Antalarmin, a novel nonpeptide CRH receptor antagonist, displaced I-125-labeled ovine CRH binding in rat pituitary, frontal cortex, and cerebellum, but not heart, consistent with antagonism at the CRHR1 receptor. In vivo antalarmin significantly inhibited CRH-stimulated ACTH release and carrageenin-induced subcutaneous inflammation in rats. Thus, antalarmin and other related compounds that antagonize CRH at the level of its own receptor have therapeutic potential in some forms of inflammation directly mediated by type 1 CRH receptors and promise to enhance our understanding of the many roles of CRH in immune/inflammatory reactions. C1 NICHHD, Pediat Endocrinol Sect, DEB, NIH, Bethesda, MD 20892 USA. RP Webster, EL (reprint author), NICHHD, Pediat Endocrinol Sect, DEB, NIH, Bldg 10,Rm 10N262, Bethesda, MD 20892 USA. EM webstere@ccl.nichd.nih.gov NR 58 TC 115 Z9 117 U1 0 U2 4 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 21 EP 32 DI 10.1111/j.1749-6632.1998.tb09545.x PG 12 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500003 PM 9629233 ER PT S AU Wilder, RL AF Wilder, RL BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI Hormones, pregnancy, and autoimmune diseases SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MARYLAND SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID RHEUMATOID-ARTHRITIS; IMMUNE-SYSTEM; FETAL; MICE; INTERLEUKIN-10; RESPONSES; CYTOKINES; PLACENTA; FAILURE; BLOOD AB Hormonal factors linked to age, gender, and reproductive status are undoubtedly involved in regulating the onset of numerous autoimmune diseases. For example, systemic lupus erythematosus (SLE), a disease characterized by immune complex-mediated pathology linked to excess Th2 cytokine production (e.g., IL-10) primarily affects women in the reproductive years. Rheumatoid arthritis (RA), a disease characterized primarily by cell-mediated joint immunopathology linked to deficient Th2 cytokine production, is also more common in women, but, in contrast to SLE, the highest incidence is at menopause. Pregnancy-associated changes in these diseases, however, provide the most compelling evidence that hormonal factors play a major role in modulating the expression of these diseases. SLE often flares during pregnancy, whereas RA commonly remits during pregnancy and flares or initially develops in the postpartum period. These observations appear to be explained by the accumulating data indicating that during pregnancy cell-mediated immune function and Th1 cytokine production (e.g., IL-12, interferon-gamma) are suppressed, and humoral immunity and Th2 cytokine production (e.g., IL-4, IL-10) are enhanced. These patterns reverse in the postpartum period. In other words, antithetical Th1/Th2 cytokine profiles appear to characterize pregnancy and the postpartum period. Strong evidence now indicates that changes in the production of cortisol, progesterone, and estrogen play a major role in modulating Th1/Th2 cytokine balance. C1 NIAMSD, Inflammatory Joint Dis Sect, NIH, Bethesda, MD 20892 USA. RP Wilder, RL (reprint author), NIAMSD, Inflammatory Joint Dis Sect, NIH, Bldg 10,Room 9N240, Bethesda, MD 20892 USA. NR 24 TC 167 Z9 171 U1 0 U2 11 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 45 EP 50 DI 10.1111/j.1749-6632.1998.tb09547.x PG 6 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500005 PM 9629235 ER PT S AU Vacchio, MS Ashwell, JD King, LB AF Vacchio, MS Ashwell, JD King, LB BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI A positive role for thymus-derived steroids in formation of the T-cell repertoire SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MD SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID PITUITARY-ADRENAL AXIS; THYMOCYTE DEVELOPMENT; NURSE CELLS; SELECTION; APOPTOSIS; ACTIVATION; DISRUPTION; COMPLEX; DEATH; NEUROSTEROIDS AB T cells undergo rigorous selection processes in the thymus that are necessary to prevent T cells with either autoreactive or nonfunctional T-cell receptors (TCRs) from entering the periphery. Although both positive and negative selection depend on TCR-mediated signals, the means by which a thymocyte interprets these signals to result in survival or death is not understood. Glucocorticoids are known to induce thymocyte apoptosis at high concentrations, but at lower concentrations glucocorticoids can antagonize TCR-mediated deletional signals and allow survival of thymocytes and T cell hybridomas. Interestingly, transgenic mice in which the expression of the glucocorticoid receptor has been downmodulated specifically in thymocytes have abnormal thymocyte differentiation, indicating that glucocorticoids play a significant role in T-cell development. Furthermore, we have demonstrated the presence of steroidogenic enzymes in the thymic epithelium and can show that, in vitro, these cells readily synthesize pregnenolone, the first product in the steroidogenic pathway, and deoxycorticosterone. Inhibition of local glucocorticoid biosynthesis in thymi from TCR transgenic mice during fetal thymic organ culture (FTOC) revealed significant alterations in the process of thymocyte selection. These data suggest that glucocorticoids do not simply suppress the immune system but rather are necessary for thymocyte survival and differentiation. C1 US FDA, Immunol Lab, Div Hematol Prod, Ctr Biol Evaluat & Res, Rockville, MD 20852 USA. NCI, Lab Immune Cell Biol, NIH, Bethesda, MD 20852 USA. Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA. RP Vacchio, MS (reprint author), 1401 Rockville Pike,HFM 538, Rockville, MD 20852 USA. EM vacchio@a1.cber.fda.gov NR 35 TC 34 Z9 35 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 317 EP 327 DI 10.1111/j.1749-6632.1998.tb09571.x PG 11 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500029 PM 9629259 ER PT S AU Anderson, NB AF Anderson, NB BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI Levels of analysis in health science - A framework for integrating sociobehavioral and biomedical research SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MARYLAND SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID PROTEIN-KINASE-C; CORTICOTROPIN-RELEASING FACTOR; TRANSCRIPTION FACTORS; CEREBRAL-CORTEX; ADULT-RATS; STRESS; EXPERIENCE; EXPRESSION; STIMULATION; INDUCTION AB One of the principal goals of the Office of Behavioral and Social Sciences Research at the National Institutes of Health is to facilitate interdisciplinary research between social, behavioral, and biomedical scientists. The purpose of this paper is to provide a framework for such interdisciplinary health research. The essence of this framework is the concept of levels of analysis in the health sciences. These levels include the social/environment, behavioral/psychological, organ systems, cellular, and molecular. The interdependence of these five levels of analysis suggests that advances in the health sciences may be accelerated by a more integrated, multilevel approach to research. The principles of integrated, multilevel research are outlined, and examples of research that support this approach are presented. Finally, some of the activities of the Office of Behavioral and Social Sciences Research that will further interdisciplinary research across levels of analysis are summarized. C1 NIH, Off Behav & Social Sci Res, Off Director, Bethesda, MD 20892 USA. RP Anderson, NB (reprint author), NIH, Off Behav & Social Sci Res, Off Director, Bldg 1,Room 326,1 Ctr Dr, Bethesda, MD 20892 USA. EM Norman_Anderson@NIH.GOV NR 57 TC 69 Z9 69 U1 5 U2 7 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 563 EP 576 DI 10.1111/j.1749-6632.1998.tb09595.x PG 14 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500053 PM 9629283 ER PT S AU Michelson, D Gold, PW AF Michelson, D Gold, PW BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI Pathophysiologic and somatic investigations of hypothalamic-pituitary-adrenal axis activation in patients with depression SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MARYLAND SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID CORTICOTROPIN-RELEASING HORMONE; BONE-MINERAL DENSITY; MULTIPLE-SCLEROSIS; ARGININE-VASOPRESSIN; MAJOR DEPRESSION; STIMULATION; DISEASE AB Preclinical studies of inflammatory and autoimmune illnesses have demonstrated the importance of central components of the HPA axis in disease pathophysiology. The implications of these data for human illness are poorly understood. We have studied the pathophysiology of the hypercortisolism seen in two human illnesses involving the central nervous system, multiple sclerosis (MS) and depression, and looked for demonstrable somatic changes that may be associated with such hypercortisolism. Data from a study of medication-free patients with multiple sclerosis not in acute exacerbation suggest that compared with depression, MS is associated with increased prominence of hypothalamic vasopressin secretion (p < 0.05). Data from studies of depressed patients with mild to moderate hypercortisolism (assessed by 24-hour urinary free cortisol excretion) demonstrate marked reductions in bone mineral density compared to healthy, carefully matched controls (p < 0.001), as well as changes in markers of bone metabolic activity similar to those seen in patients with Cushing's disease or exogenous glucocorticoid treatment (p < 0.05). Taken together, these studies suggest HPA axis dysregulations demonstrated in preclinical models of autoimmune and inflammatory illness also occur in human illness and may have important and lasting somatic sequelae. C1 NIMH, Clin Neuroendocrinol Branch, Bethesda, MD 20892 USA. RP Michelson, D (reprint author), Eli Lilly & Co, Lilly Corp Ctr 2423, Indianapolis, IN 46285 USA. NR 15 TC 15 Z9 15 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 717 EP 722 DI 10.1111/j.1749-6632.1998.tb09610.x PG 6 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500068 PM 9629298 ER PT S AU Leung, DYM De Castro, M Szefler, SJ Chrousos, GP AF Leung, DYM De Castro, M Szefler, SJ Chrousos, GP BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI Mechanisms of glucocorticoid-resistant asthma SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MARYLAND SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID PRIMARY CORTISOL RESISTANCE; RECEPTOR-BINDING AFFINITY; NF-KAPPA-B; BRONCHOALVEOLAR LAVAGE; BRONCHIAL-ASTHMA; GENE-EXPRESSION; MESSENGER-RNA; INHIBITION; CELLS; DNA AB The term "steroid- or glucocorticoid-resistant (GR) asthma" has been used to describe a group of asthmatics who demonstrate persistent airway obstruction and inflammation despite treatment with high doses of systemic glucocorticoids (GCs). There are at least two forms of GR asthma. The first group of patients has an acquired form of GC resistance. Analysis of their bronchoalveolar lavage (BAL) cells indicates an extremely high level of IL-2 and IL-4 gene expression as compared to BAL cells from CC-sensitive asthmatics. The incubation of T cells from normal individuals with the combination of IL-2 and IL-4 induces GC receptor binding defects, and concomitant GC receptor beta expression, in their cells. Similar abnormalities in GC receptor binding defects can be detected in freshly isolated cells from type 1 GR asthmatics, but this defect is reversible when their cells are cultured in the absence of IL-2 and IL-4. The second group involves patients with primary GC resistance who do not develop side effects on high-dose GCs and have very low numbers of GC receptors in their mononuclear cells. This defect is irreversible in culture and affects their T cells as well as non-T cells. The current studies provide new insights into mechanisms by which inflammation induces GC resistance and how defects in the GC receptor may contribute to chronic inflammation, creating the vicious cycle leading to chronic inflammatory diseases. C1 Natl Jewish Ctr Immunol & Resp Med, Div Pediat Allergy Immunol, Denver, CO 80206 USA. Natl Jewish Ctr Immunol & Resp Med, Div Clin Pharmacol, Denver, CO 80206 USA. Univ Colorado, Hlth Sci Ctr, Dept Pediat, Denver, CO 80206 USA. NIH, Dev Endocrinol Branch, Bethesda, MD 20892 USA. RP Leung, DYM (reprint author), Natl Jewish Ctr Immunol & Resp Med, Div Pediat Allergy Immunol, 1400 Jackson St, Denver, CO 80206 USA. RI Castro, Margaret/A-4918-2009 FU NCRR NIH HHS [RR-00051]; NHLBI NIH HHS [HL-36577]; NIAMS NIH HHS [AR-41256] NR 49 TC 55 Z9 56 U1 0 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 735 EP 746 DI 10.1111/j.1749-6632.1998.tb09612.x PG 12 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500070 PM 9629300 ER PT S AU Reincke, M Arlt, W Heppner, C Petzke, F Chrousos, GP Allolio, B AF Reincke, M Arlt, W Heppner, C Petzke, F Chrousos, GP Allolio, B BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI Neuroendocrine dysfunction in African trypanosomiasis - The role of cytokines SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MARYLAND SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID TUMOR-NECROSIS-FACTOR; CORTICOTROPIN-RELEASING HORMONE; PITUITARY-ADRENAL AXIS; FACTOR-ALPHA-CACHECTIN; BRUCEI INFECTION; THYROID-FUNCTION; CENTRAL HYPOTHYROIDISM; RHEUMATOID-ARTHRITIS; SLEEPING SICKNESS; CRITICAL ILLNESS AB Sleeping sickness (SS; African trypanosomiasis) is an anthropozoonosis transmitted by the tsetse fly. Infection with Trypanosoma brucei in humans is associated with adynamia, lethargy, anorexia, and more specifically amenorrhea/infertility in women and loss of libido/impotence in men. Recent evidence suggests that experimental infection in animals with Trypanosoma brucei species causes polyglandular endocrine failure by local inflammation of the pituitary, thyroid, adrenal, and gonadal glands. In a cross-sectional study we investigated the prevalence and significance of neuroendocrine abnormalities in 137 Ugandan patients with SS. In the untreated stage of the disease, there was a high prevalence of adrenal insufficiency (27%), hypothyroidism (50%) and hypogonadism (85%). Pituitary function tests suggested an unusual combined central (hypothalamic/pituitary) and peripheral defect in hormone secretion. Specific therapy resulted in a rapid recovery of adrenal/thyroid function, whereas hypogonadism persisted for years in a substantial portion of patients. We did not detect pituitary, thyroid, adrenal, and gonadal autoantibodies in patients with endocrine dysfunction, ruling out an autoimmune origin of the endocrine abnormalities. However, the presence of hypopituitarism correlated with high cytokine concentrations (TNF-alpha, IL-6) which-together with direct parasitic infiltration of the endocrine glands-are involved in the pathogenesis of SS-associated endocrine dysfunction. C1 Univ Wurzburg, Med Klin, Dept Med, Endocrinol Sect, D-97080 Wurzburg, Germany. NICHHD, Dev Endocrinol Branch, Bethesda, MD 20892 USA. RP Reincke, M (reprint author), Univ Wurzburg, Med Klin, Dept Med, Endocrinol Sect, Josef Schneider Str 2, D-97080 Wurzburg, Germany. RI Arlt, Wiebke/B-6310-2009 OI Arlt, Wiebke/0000-0001-5106-9719 NR 53 TC 26 Z9 26 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 809 EP 821 DI 10.1111/j.1749-6632.1998.tb09619.x PG 13 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500077 PM 9629307 ER PT S AU Sei, Y Kustova, Y Morse, HC Skolnick, P Basile, AS AF Sei, Y Kustova, Y Morse, HC Skolnick, P Basile, AS BE McCann, SM Lipton, JM Sternberg, EM Chrousos, GP Gold, PW Smith, CC TI The encephalopathy associated with murine acquired immunodeficiency syndrome SO NEUROIMMUNOMODULATION: MOLECULAR ASPECTS, INTEGRATIVE SYSTEMS, AND CLINICAL ADVANCES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 3rd Congress of the International-Society-of-Neuroimmunomodulation on Neuroimmunomodulation - Molecular Aspects, Integrative Systems, and Clinical Advances CY NOV 13-15, 1996 CL BETHESDA, MARYLAND SP Int Soc Neuroimmunomodulat, NIH, NCI, Natl Inst Aging, NIAID, Natl Inst Arthritis & Muskuloskeletal & Skin Dis, NICHHD, NIDR, NIMH, Natl Inst Nursing Res, NIH, Off Alternat Med, NIH, Off Behav & Social Sci Res, NIH, Off Rare Dis Res, NIH, Off Res Womens Hlth, Glaxowellcome Inc, TIG Instrumenten Gesell Ag, Vector Lab ID RETROVIRUS-INDUCED IMMUNODEFICIENCY; CENTRAL-NERVOUS-SYSTEM; PLATELET-ACTIVATING-FACTOR; QUINOLINIC ACID; MICE; CELLS; VIRUS; BRAIN; FYN; MAIDS AB Mice infected with the LP-BM5 murine leukemia virus (MuLV) develop an immune deficiency syndrome together with an encephalopathy characterized by impairments in spatial learning and memory. These cognitive deficits are evident before the appearance of neuron loss and lymphoid cell invasion of the brain. Nonetheless, a prominent gliosis and a variety of neurochemical changes precede the development of cognitive deficits. The neurochemical abnormalities include significant decreases in striatal Met-enkephalin and substance P (but not somatostatin), increases in concentrations of quinolinic acid and platelet-activating factor, and alterations in brain fyn kinase. At this stage of the infection, some of these neurochemical changes can be reversed by glutamate receptor antagonists, cytokine inhibitors, and anti-retroviral agents. In later stages of the infection, however, the infected mice develop irreversible neuronal loss, invasion of hematopoietic cells, and increased viral burden in the CNS. In addition, motor-neuron dysfunction (hindlimb paralysis, weakness, and ataxia) and seizures are sometimes observed during the late stages of infection. Thus, the LP-BM5 MuLV-infected mouse is a useful model for studying the chronology of neurodegenerative changes, ranging from reversible neuron dysfunction to irreversible neuron loss, that are associated with retrovirus-induced immunodeficiency. C1 Uniformed Serv Univ Hlth Sci, Dept Anesthesiol, Bethesda, MD 20892 USA. NIAID, Immunopathol Lab, NIH, Bethesda, MD 20892 USA. NIDDK, Neurosci Lab, NIH, Bethesda, MD 20892 USA. RP Sei, Y (reprint author), Uniformed Serv Univ Hlth Sci, Dept Anesthesiol, Bethesda, MD 20892 USA. OI Morse, Herbert/0000-0002-9331-3705 NR 35 TC 12 Z9 12 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-072-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 840 BP 822 EP 834 DI 10.1111/j.1749-6632.1998.tb09620.x PG 13 WC Endocrinology & Metabolism; Immunology; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Immunology; Science & Technology - Other Topics; Neurosciences & Neurology GA BL14S UT WOS:000074444500078 PM 9629308 ER PT J AU Marx, C Wolkersdorfer, GW Bornstein, SR AF Marx, C Wolkersdorfer, GW Bornstein, SR TI A new view on immune-adrenal interactions: Role for Fas and Fas ligand? SO NEUROIMMUNOMODULATION LA English DT Editorial Material DE adrenal; apoptosis; Fas; Fas ligand; glucocorticoids; dehydroepiandrosterone sulfate; zonation; stress; immune system ID LYMPHOCYTES; APOPTOSIS AB The hypothalamic-pituitary-adrenal axis and the immune system interact in a bidirectional manner providing the basis for the regulation of the immune response due to a pathogenic stimulus. This interplay is commonly believed to be based on the action of hormones or cytokines, respectively. Since it has been detected that adrenocortical cells offer immunological properties such as expression of MHC class II antigens and/or CD95 (Fas antigen) and its ligand, the question has to be raised whether direct intercellular communication between immune cells and 'immunocompetent' endocrine cells contributes to the complexity of immunoregulation. Here we discuss the possible reciprocal relevance of physiological and pathological adrenal changes, as well as T-cell-mediated immune response for immune and/or adrenal pathology during disease. C1 NICHHD, Dev Endocrinol Branch, Ctr Clin, NIH, Bethesda, MD 20892 USA. Univ Leipzig, Dept Internal Med 3, D-7010 Leipzig, Germany. RP Bornstein, SR (reprint author), NICHHD, Dev Endocrinol Branch, Ctr Clin, NIH, Bldg 10,Room 10N262, Bethesda, MD 20892 USA. NR 16 TC 15 Z9 15 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1021-7401 J9 NEUROIMMUNOMODULAT JI Neuroimmunomodulation PD JAN-APR PY 1998 VL 5 IS 1-2 BP 5 EP 8 DI 10.1159/000026320 PG 4 WC Endocrinology & Metabolism; Immunology; Neurosciences SC Endocrinology & Metabolism; Immunology; Neurosciences & Neurology GA 100ZR UT WOS:000074845600002 PM 9698252 ER PT J AU Litvan, I AF Litvan, I TI Regressive supranuclear palsy: Staring into the past, moving into the future SO NEUROLOGIST LA English DT Article DE progressive supranuclear palsy; neurodegenerative disorders; therapies; pathogenesis ID RICHARDSON-OLSZEWSKI-SYNDROME; MULTIPLE SYSTEM ATROPHY; POSITRON EMISSION TOMOGRAPHY; PROGRESSIVE SUBCORTICAL GLIOSIS; PARKINSONS-DISEASE; NEURODEGENERATIVE DISORDERS; STRIATONIGRAL DEGENERATION; NATURAL-HISTORY; NEUROPSYCHOLOGICAL PATTERN; CORTICOBASAL DEGENERATION AB OBJECTIVE-To review clinical and basic research on progressive supranuclear palsy. METHODS-MEDLINE literature review search from 1966 to 1997. CONCLUSIONS-Significant progress has occurred since Steele, Richardson, and Olzewski first described this disorder as a specific clinicopathologic entity 33 years ago. Clinical features, laboratory testing, and therapeutic strategies are discussed. In addition, hypothesized pathogenetic mechanisms that may help in the search for appropriate biologic therapies are considered. C1 NINDS, Jackson Fdn & Med Neurol Branch, Def & Vet Head Injury Program Neuropharmacol Unit, NIH, Bethesda, MD 20892 USA. OI Litvan, Irene/0000-0002-3485-3445 NR 96 TC 5 Z9 5 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 1074-7931 J9 NEUROLOGIST JI Neurologist PD JAN PY 1998 VL 4 IS 1 BP 13 EP 20 DI 10.1097/00127893-199801000-00003 PG 8 WC Clinical Neurology SC Neurosciences & Neurology GA YV853 UT WOS:000071869900002 ER PT J AU Flitman, SS Grafman, J Wassermann, EM Cooper, V O'Grady, J Pascual-Leone, A Hallett, M AF Flitman, SS Grafman, J Wassermann, EM Cooper, V O'Grady, J Pascual-Leone, A Hallett, M TI Linguistic processing during repetitive transcranial magnetic stimulation SO NEUROLOGY LA English DT Article ID SPEECH; BRAIN; INDUCTION; LANGUAGE; COMPREHENSION; EPILEPSY; ERRORS AB To determine if linguistic processing could be selectively disrupted with repetitive transcranial magnetic stimulation (rTMS), rTMS was performed during a picture-word verification task. Seven right-handed subjects were trained in two conditions: picture-word verification, which required the subject to verify whether the picture of an object matched the subtitle name on the same page, and frame verification, which required subjects to verify whether there was a rectangular frame around the combined object picture and subtitle. Half of the trials were performed during rTMS. The effects of rTMS on performance were evaluated at the following four scalp positions: left anterior (the area where rTMS produced speech arrest), a mirror site on the right, and two positions in the left and right parietal region. Stimulation over the left deltoid muscle served as a control. Subjects had less difficulty in making picture-word matching decisions during unstimulated compared with stimulated trials at the left anterior and posterior positions. No significant difference in accuracy was detected in the frame verification condition, but response times in the frame verification condition were longer with stimulation at the left anterior position. Because rTMS of the dominant hemisphere affected linguistic processing independent of speech motor output, we confirm that rTMS may be used to investigate language and other cognitive functions. C1 NINDS, Cognit Neurosci Sect, NIH, MNB, Bethesda, MD 20892 USA. NINDS, Human Motor Control Sect, Bethesda, MD 20892 USA. Harvard Univ, Sch Med, Deaconess Beth Israel Med Ctr, Dept Neurol, Boston, MA USA. CSIC, Inst Cajal, E-28002 Madrid, Spain. RP Grafman, J (reprint author), NINDS, Cognit Neurosci Sect, NIH, MNB, 10 Ctr Dr,MSC 1440,Bldg 10,Room 5C205, Bethesda, MD 20892 USA. RI Pascual-Leone, Alvaro/G-6566-2011; OI Grafman, Jordan H./0000-0001-8645-4457 NR 28 TC 96 Z9 99 U1 1 U2 4 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0028-3878 J9 NEUROLOGY JI Neurology PD JAN PY 1998 VL 50 IS 1 BP 175 EP 181 PG 7 WC Clinical Neurology SC Neurosciences & Neurology GA YR353 UT WOS:000071486900032 PM 9443476 ER PT J AU Higgins, JJ Litvan, I Pho, LT Li, W Nee, LE AF Higgins, JJ Litvan, I Pho, LT Li, W Nee, LE TI Progressive supranuclear gaze palsy is in linkage disequilibrium with the tau and not the alpha-synuclein gene SO NEUROLOGY LA English DT Article ID DISEASE AB We studied two candidate genes, tau (tau) and alpha-synuclein (SNCA), for evidence of linkage disequilibrium on a group of unrelated individuals with progressive supranuclear palsy (PSP) and a group of age-matched control subjects. The tau a(1) allele and the tau a(1)a(1) genotype were overrepresented in individuals with PSP and the tau polymorphism was in linkage disequilibrium with the PSP disease locus when a recessive inheritance model was employed. We also report a lack of evidence to support linkage disequilibrium between PSP and the SNCA candidate Parkinson's disease gene on chromosome 4q21-q23. C1 NINDS, Med Neurol Branch, Clin Neurogenet Unit, NIH, Bethesda, MD 20892 USA. NINDS, Neuroepidemiol Branch, NIH, Bethesda, MD 20892 USA. Rockefeller Univ, Lab Stat Genet, New York, NY 10021 USA. RP Higgins, JJ (reprint author), New York State Dept Hlth, Wadsworth Ctr, POB 509, Albany, NY 12201 USA. NR 13 TC 87 Z9 89 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0028-3878 J9 NEUROLOGY JI Neurology PD JAN PY 1998 VL 50 IS 1 BP 270 EP 273 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA YR353 UT WOS:000071486900047 PM 9443491 ER PT J AU Samii, A Chen, R Wassermann, EM Hallett, M AF Samii, A Chen, R Wassermann, EM Hallett, M TI Phenytoin does not influence postexercise facilitation of motor evoked potentials SO NEUROLOGY LA English DT Article ID TRANSCRANIAL MAGNETIC STIMULATION; LONG-TERM POTENTIATION; EXCITABILITY; HIPPOCAMPUS; DEPRESSION; CORTEX AB Postexercise facilitation of motor evoked potentials (MEPs) elicited to transcranial magnetic stimulation occurs after brief, non-fatiguing muscle activation. This phenomenon may be related to post-tetanic potentiation or long-term potentiation (LTP) observed in animal studies. Phenytoin reduces post-tetanic potentiation but does not block LTP. We studied the effects of phenytoin on postexercise MEP facilitation and its decay over time. Phenytoin did not result in either significant change in postexercise MEP facilitation nor significant change in the decay of facilitation. We conclude that postexercise MEP facilitation is unlikely to be secondary to posttetanic potentiation. C1 NINDS, Human Motor Control Sect, Med Neurol Branch, NIH, Bethesda, MD 20892 USA. RP Hallett, M (reprint author), NINDS, Human Motor Control Sect, Med Neurol Branch, NIH, Bldg 10,Room 5N226,10 Ctr Dr MSC 1428, Bethesda, MD 20892 USA. RI Chen, Robert/B-3899-2009 OI Chen, Robert/0000-0002-8371-8629 NR 10 TC 5 Z9 6 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0028-3878 J9 NEUROLOGY JI Neurology PD JAN PY 1998 VL 50 IS 1 BP 291 EP 293 PG 3 WC Clinical Neurology SC Neurosciences & Neurology GA YR353 UT WOS:000071486900054 PM 9443498 ER PT B AU Richmond, BJ Gawne, TJ AF Richmond, BJ Gawne, TJ BE Eichenbaum, HB Davis, JL TI The relationship between neuronal codes and cortical organization SO NEURONAL ENSEMBLES: STRATEGIES FOR RECORDING AND DECODING LA English DT Proceedings Paper CT Office of Naval Research Workshop CY MAR 22-23, 1996 CL BOSTON UNIV, BOSTON, MA SP Off Naval Res HO BOSTON UNIV C1 NIMH, Neuropsychol Lab, Bethesda, MD 20892 USA. RP Richmond, BJ (reprint author), NIMH, Neuropsychol Lab, Bldg 9, Bethesda, MD 20892 USA. NR 0 TC 5 Z9 5 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 USA BN 0-471-17940-X PY 1998 BP 57 EP 79 PG 23 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA BL25H UT WOS:000074897200003 ER PT S AU O'Donovan, MJ Wenner, P Chub, N Tabak, J Rinzel, J AF O'Donovan, MJ Wenner, P Chub, N Tabak, J Rinzel, J BE Kiehn, O HarrisWarrick, RM Jordan, LM Hultborn, H Kudo, N TI Mechanisms of spontaneous activity in the developing spinal cord and their relevance to locomotion SO NEURONAL MECHANISMS FOR GENERATING LOCOMOTOR ACTIVITY SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Neuronal Mechanisms for Generating Locomotor Activity CY MAR 20-23, 1998 CL NEW YORK, NEW YORK SP New York Acad Sci, Athena Neurosci Inc, E Paralyzed Vet Assoc, Lundbeck Fdn, NIH, NINDS, NIH, NIA, Natl Sci Fdn, Spinal Cord Res Fdn, Univ Tsukuba, Japan, Med Res Council Canada, Univ Copenhagens Gen Fdn ID CHICK-EMBRYO; SYNAPTIC DRIVE; MOTOR-ACTIVITY; MOTONEURONS; GABA; DEPRESSION; GLYCINE; GENERATION; NETWORKS; NEURONS AB The isolated humbosacral cord of the chick embryo generates spontaneous episodes of rhythmic activity. Muscle nerve recordings show that the discharge of sartorius (flexor) and fermorotibialis (extensor) motoneurons alternates even though the motoneurons are depolarized simultaneously during each cycle. The alternation occurs because sartorius motoneuron firing is shunted or voltage-clamped by its synaptic drive at the time of peak femorotibialis discharge. Ablation experiments have identified a region dorsomedial to the lateral motor column that may be required for the alternation of sartorius and femorotibialis motoneurons. This region overlaps the location of interneurons activated by ventral root stimulation. Whole-cell recordings from interneurons receiving short latency ventral root input indicate that they lire at an appropriate time to contribute to the cyclical pause in firing of sartorius motoneurons. Spontaneous activity was modeled by the interaction of three variables: network activity and two activity-dependent forms of network depression.. A "slow" depression which regulates the occurrence of episodes and a "fast" depression that controls cycling during an episode. The model successfully predicts several aspects of spinal network behavior including spontaneous rhythmic activity and the recovery of network activity following blockade of excitatory synaptic transmission. C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. NYU, Ctr Neural Sci, New York, NY 10003 USA. NYU, Courant Inst Math Sci, New York, NY 10003 USA. RP O'Donovan, MJ (reprint author), NINDS, Neural Control Lab, NIH, Bldg 49,Room 3A50, Bethesda, MD 20892 USA. EM odonovan@codon.nih.gov RI tabak, joel/K-1549-2013; OI Wenner, Peter/0000-0002-7072-2194 NR 33 TC 49 Z9 49 U1 0 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-168-5 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 860 BP 130 EP 141 PG 12 WC Behavioral Sciences; Multidisciplinary Sciences; Neurosciences SC Behavioral Sciences; Science & Technology - Other Topics; Neurosciences & Neurology GA BM35B UT WOS:000078450500010 PM 9928307 ER PT S AU Wenner, P Matise, MP Joyner, A O'Donovan, MJ AF Wenner, P Matise, MP Joyner, A O'Donovan, MJ BE Kiehn, O HarrisWarrick, RM Jordan, LM Hultborn, H Kudo, N TI Physiological and molecular characterization of interneurons in the developing spinal cord SO NEURONAL MECHANISMS FOR GENERATING LOCOMOTOR ACTIVITY SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Neuronal Mechanisms for Generating Locomotor Activity CY MAR 20-23, 1998 CL NEW YORK, NEW YORK SP New York Acad Sci, Athena Neurosci Inc, E Paralyzed Vet Assoc, Lundbeck Fdn, NIH, NINDS, NIH, NIA, Natl Sci Fdn, Spinal Cord Res Fdn, Univ Tsukuba, Japan, Med Res Council Canada, Univ Copenhagens Gen Fdn C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. NYU, Med Ctr, Skirball Inst, New York, NY 10016 USA. RP Wenner, P (reprint author), NINDS, Neural Control Lab, NIH, Bldg 49,Room 3A50, Bethesda, MD 20892 USA. OI Wenner, Peter/0000-0002-7072-2194 NR 5 TC 11 Z9 11 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-168-5 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 860 BP 425 EP 427 DI 10.1111/j.1749-6632.1998.tb09066.x PG 3 WC Behavioral Sciences; Multidisciplinary Sciences; Neurosciences SC Behavioral Sciences; Science & Technology - Other Topics; Neurosciences & Neurology GA BM35B UT WOS:000078450500032 PM 9928329 ER PT S AU Tabak, J O'Donovan, MJ AF Tabak, J O'Donovan, MJ BE Kiehn, O HarrisWarrick, RM Jordan, LM Hultborn, H Kudo, N TI Statistical analysis and intersegmental delays reveal possible roles of network depression in the generation of spontaneous activity in the chick embryo spinal cord SO NEURONAL MECHANISMS FOR GENERATING LOCOMOTOR ACTIVITY SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Neuronal Mechanisms for Generating Locomotor Activity CY MAR 20-23, 1998 CL NEW YORK, NEW YORK SP New York Acad Sci, Athena Neurosci Inc, E Paralyzed Vet Assoc, Lundbeck Fdn, NIH, NINDS, NIH, NIA, Natl Sci Fdn, Spinal Cord Res Fdn, Univ Tsukuba, Japan, Med Res Council Canada, Univ Copenhagens Gen Fdn C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. RP Tabak, J (reprint author), NINDS, Neural Control Lab, NIH, Bldg 49,Room 3A50, Bethesda, MD 20892 USA. RI tabak, joel/K-1549-2013 NR 4 TC 7 Z9 7 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-168-5 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 860 BP 428 EP 431 DI 10.1111/j.1749-6632.1998.tb09067.x PG 4 WC Behavioral Sciences; Multidisciplinary Sciences; Neurosciences SC Behavioral Sciences; Science & Technology - Other Topics; Neurosciences & Neurology GA BM35B UT WOS:000078450500033 PM 9928330 ER PT S AU Bonnot, A Morin, D Viala, D AF Bonnot, A Morin, D Viala, D BE Kiehn, O HarrisWarrick, RM Jordan, LM Hultborn, H Kudo, N TI Organization of rhythmic motor patterns in the lumbosacral spinal cord of neonate mouse SO NEURONAL MECHANISMS FOR GENERATING LOCOMOTOR ACTIVITY SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Neuronal Mechanisms for Generating Locomotor Activity CY MAR 20-23, 1998 CL NEW YORK, NEW YORK SP New York Acad Sci, Athena Neurosci Inc, E Paralyzed Vet Assoc, Lundbeck Fdn, NIH, NINDS, NIH, NIA, Natl Sci Fdn, Spinal Cord Res Fdn, Univ Tsukuba, Japan, Med Res Council Canada, Univ Copenhagens Gen Fdn ID RAT; LOCALIZATION C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. Univ Bordeaux 1, Lab Neurosci Motricite, CNRS, UMR 5807, F-33405 Talence, France. RP Bonnot, A (reprint author), NINDS, Neural Control Lab, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA. RI Morin, Didier/C-6651-2014 NR 5 TC 8 Z9 8 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-168-5 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 860 BP 432 EP 435 DI 10.1111/j.1749-6632.1998.tb09068.x PG 4 WC Behavioral Sciences; Multidisciplinary Sciences; Neurosciences SC Behavioral Sciences; Science & Technology - Other Topics; Neurosciences & Neurology GA BM35B UT WOS:000078450500034 PM 9928331 ER PT S AU Chub, N Moore, LE O'Donovan, MJ AF Chub, N Moore, LE O'Donovan, MJ BE Kiehn, O HarrisWarrick, RM Jordan, LM Hultborn, H Kudo, N TI Comparison of NMDA-induced membrane potential oscillations and spontaneous rhythmic activity in the chick spinal cord SO NEURONAL MECHANISMS FOR GENERATING LOCOMOTOR ACTIVITY SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Neuronal Mechanisms for Generating Locomotor Activity CY MAR 20-23, 1998 CL NEW YORK, NEW YORK SP New York Acad Sci, Athena Neurosci Inc, E Paralyzed Vet Assoc, Lundbeck Fdn, NIH, NINDS, NIH, NIA, Natl Sci Fdn, Spinal Cord Res Fdn, Univ Tsukuba, Japan, Med Res Council Canada, Univ Copenhagens Gen Fdn C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. RP Chub, N (reprint author), NINDS, Neural Control Lab, NIH, Bldg 49,Room 3A50, Bethesda, MD 20892 USA. NR 5 TC 3 Z9 3 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-168-5 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 860 BP 467 EP 469 DI 10.1111/j.1749-6632.1998.tb09078.x PG 3 WC Behavioral Sciences; Multidisciplinary Sciences; Neurosciences SC Behavioral Sciences; Science & Technology - Other Topics; Neurosciences & Neurology GA BM35B UT WOS:000078450500044 PM 9928341 ER PT J AU Post, RM Denicoff, KD Frye, MA Dunn, RT Leverich, GS Osuch, E Speer, A AF Post, RM Denicoff, KD Frye, MA Dunn, RT Leverich, GS Osuch, E Speer, A TI A history of the use of anticonvulsants as mood stabilizers in the last two decades of the 20th century SO NEUROPSYCHOBIOLOGY LA English DT Article; Proceedings Paper CT Symposium on New Antiepileptic Drugs in Psychiatry CY MAR 27-29, 1998 CL SCHLOSS ELMAU, GERMANY SP Smith Kline Beecham Fdn, Glaxo Wellcome DE bipolar illness; mania; refractory depression; lithium ID THYROTROPIN-RELEASING-HORMONE; AMYGDALA-KINDLED SEIZURES; TRANSCRANIAL MAGNETIC STIMULATION; MANIC-DEPRESSIVE ILLNESS; BIPOLAR DISORDER; CONTINGENT TOLERANCE; SLEEP-DEPRIVATION; ELECTROCONVULSIVE-THERAPY; CARBAMAZEPINE; VALPROATE AB Anticonvulsants have moved into an important position as alternatives and adjuncts to lithium carbonate in the treatment of bipolar illness. Work with the nonhomologous model of kindled seizures helped in the choice of carbamazepine as a potential mood stabilizer and in the study of the mechanisms of action of the second generation anticonvulsants carbamazepine and valproate, as well as the putative third generation psychotropic anticonvulsants lamotrigine and gabapentin. Anticonvulsant neuropeptides such as TRH and nonconvulsant approaches with repeated transcranial magnetic stimulation (rTMS) also appear promising. C1 NIMH, Biol Psychiat Branch, NIH, Bethesda, MD 20892 USA. RP Post, RM (reprint author), NIMH, Biol Psychiat Branch, NIH, Bldg 10,Room 3N212,10 Ctr Dr MSC 1272, Bethesda, MD 20892 USA. RI Osuch, Elizabeth/B-5009-2015 OI Osuch, Elizabeth/0000-0001-5946-1862 NR 112 TC 67 Z9 68 U1 1 U2 4 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0302-282X J9 NEUROPSYCHOBIOLOGY JI Neuropsychobiology PY 1998 VL 38 IS 3 BP 152 EP 166 DI 10.1159/000026532 PG 15 WC Neurosciences; Psychiatry; Psychology SC Neurosciences & Neurology; Psychiatry; Psychology GA 133LL UT WOS:000076688000007 PM 9778604 ER PT J AU Weiss, SRB Post, RM AF Weiss, SRB Post, RM TI Kindling: Separate vs. shared mechanisms in affective disorders and epilepsy SO NEUROPSYCHOBIOLOGY LA English DT Article; Proceedings Paper CT Symposium on New Antiepileptic Drugs in Psychiatry CY MAR 27-29, 1998 CL SCHLOSS ELMAU, GERMANY SP Smith Kline Beecham Fdn, Glaxo Wellcome DE amygdala; tolerance; anticonvulsant; seizures; psychiatry; review ID THYROTROPIN-RELEASING-HORMONE; LONG-TERM POTENTIATION; TRANSCRANIAL MAGNETIC STIMULATION; POSITRON EMISSION TOMOGRAPHY; CONTINGENT TOLERANCE; SYNAPTIC PLASTICITY; SPONTANEOUS SEIZURES; SYMPTOM PROVOCATION; DEPRESSED-PATIENTS; PREFRONTAL CORTEX AB Kindling is discussed in relation to affective illness as a nonhomologous model, which shares the feature of increasing illness severity and evolution over time following repeated exposures to certain forms of stimulation. This progressive aspect of kindling has proven useful in the study of approaches to pharmacotherapeutics, mechanisms and characteristics of drug tolerance, and, most recently, illness suppression through physiological rather than pharmacological strategies. Each of these themes is described and the mechanisms that have been uncovered using the kindling model are discussed in relation to how similar principles might apply in affective illness or epilepsy. It is hoped that some of the lessons from the kindling model will provide useful and novel insights into aspects of treatment and mechanisms of psychiatric and neurologic illnesses. C1 NIMH, BPB, Bethesda, MD 20892 USA. RP Weiss, SRB (reprint author), NIMH, BPB, Bldg 10-3N212,9000 Rockville Pike, Bethesda, MD 20892 USA. EM SRBW@Sparky.NIMH.NIH.GOV NR 86 TC 40 Z9 40 U1 1 U2 2 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0302-282X J9 NEUROPSYCHOBIOLOGY JI Neuropsychobiology PY 1998 VL 38 IS 3 BP 167 EP 180 DI 10.1159/000026533 PG 14 WC Neurosciences; Psychiatry; Psychology SC Neurosciences & Neurology; Psychiatry; Psychology GA 133LL UT WOS:000076688000008 PM 9778605 ER PT J AU Bertolino, A Callicott, JH Nawroz, S Mattay, VS Duyn, JH Tedeschi, G Frank, JA Weinberger, DR AF Bertolino, A Callicott, JH Nawroz, S Mattay, VS Duyn, JH Tedeschi, G Frank, JA Weinberger, DR TI Reproducibility of proton magnetic resonance spectroscopic imaging in patients with schizophrenia SO NEUROPSYCHOPHARMACOLOGY LA English DT Article DE frontal cortex; hippocampus; N-acetyl-aspartate ID TEMPORAL-LOBE EPILEPSY; IN-VIVO; HUMAN-BRAIN; H-1-NMR SPECTROSCOPY; MULTIPLE-SCLEROSIS; FRONTAL LOBES; INVIVO; METABOLITES; PATTERN; THERAPY AB Using proton magnetic resonance spectroscopic imaging (H-1-MRSI) we found in a previous study a specific pattern of neuronal pathology in patients with schizophrenia as determined by relative loss of signal from N-acetyl-containing compounds (NAA). The purpose of the present study was to assess the reproducibility of the results of H-1-MRSI both in patients with schizophrenia and in normal controls. We studied twice 10 patients and 10 controls on 2 days separated by, on average, 3 months. Reproducibility was assessed with several statistical procedures including ANOVA, coefficients of variation (CVs) and intra-class correlation coefficients (ICC). Patients showed significant reductions of NAA/creatine-phosphocreatine (CRE) and NAA/choline-containing compounds (CHO) selectively in the hippocampal region (HIPPO) and in the dorsolateral prefrontal cortex (DLPFC) on both experimental days. A repeated measures ANOVA showed no effect of time on metabolite ratios in all subjects. CVs were fairly low (especially for NAA/CRE and CHO/CRE) and did not differ significantly between patients and controls. The ICCs of the ROIs reached statistical significance only in a few instances. The present multislice H-1-MRSI study shows that: (1) patients with schizophrenia, when compared as it group to normal controls, show a consistent H-1-MRSI pattern of group differences, i.e., bilateral reductions of NAA/CRE and NAA/CHO in HIPPO and DLPFC; (2) H-1-MRSI data in both patients and controls do not show significant changes over this 90-day period; however, absolute metabolite ratios in individuals show low predictability over this time interval; (3) H-1-MRSI data show relatively low variability (ns measured by the CVs) both in patients and normal controls, especially for NAA/CRE and CHO/CRE. Published by Elsevier Science Inc. C1 NIMH,CLIN BRAIN DISORDERS BRANCH,INTRAMURAL RES PROGRAM,NIH,ST ELIZABETHS,NEUROSCI CTR,WASHINGTON,DC 20032. NINCDS,NEUROIMAGING BRANCH,NIH,WASHINGTON,DC. LAB DIAGNOST RADIOL RES,OD,NIH,WASHINGTON,DC. RI Duyn, Jozef/F-2483-2010; Callicott, Joseph/C-9102-2009; Bertolino, Alessandro/O-6352-2016 OI Callicott, Joseph/0000-0003-1298-3334; Bertolino, Alessandro/0000-0002-1251-1380 NR 38 TC 62 Z9 63 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0893-133X J9 NEUROPSYCHOPHARMACOL JI Neuropsychopharmacology PD JAN PY 1998 VL 18 IS 1 BP 1 EP 9 PG 9 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA YJ479 UT WOS:A1998YJ47900001 PM 9408913 ER PT J AU Mele, A Thomas, DN Pert, A AF Mele, A Thomas, DN Pert, A TI Different neural mechanisms underlie dizocilpine maleate- and dopamine agonist-induced locomotor activity SO NEUROSCIENCE LA English DT Article DE GABA; amphetamine; MK-801; nucleus accumbens; ventral pallidum ID MEDIAL PREFRONTAL CORTEX; NMDA RECEPTOR ANTAGONISTS; NUCLEUS-ACCUMBENS; BASAL GANGLIA; PEDUNCULOPONTINE NUCLEUS; INVIVO MICRODIALYSIS; EXTRACELLULAR GABA; VENTRAL PALLIDUM; 6-OHDA LESIONS; RAT AB This study evaluated and compared the role of mesoaccumbens dopamine and the ventral pallidal region in the locomotor stimulatory action of the non-competitive N-methyl-D-aspartate antagonist dizocilpine maleate and dopamine agonists. Intra-accumbens injections of both amphetamine (1, 5 and 25 nmol) and dizocilpine maleate (1, 5, 25 and 50 nmol) induced a dose-dependent increase in locomotor activity. The N-methyl-D-aspartate antagonist was somewhat less effective than amphetamine. 6-Hydroxydopamine dopamine-depleting lesions of the nucleus accumbens completely blocked locomotor stimulation induced by focal administrations of amphetamine (5 nmol), but were ineffective in altering the actions of dizocilpine maleate (50 nmol). Ibotenic acid lesions of the ventral pallidal region and muscimol injections into this area also prevented the stimulatory effects of systemic amphetamine (1 mg/kg), while having no effect on the locomotor-activating actions of systemic dizocilpine maleate (0.3 mg/kg). Microdialysis studies revealed that systemically administred apomorphine (2 mg/kg) significantly decreased extracellular GABA in the pallidum, which was accompained by substantial increases in locomotor output. Systemically administred dizocilpine maleate (0.3 mg/kg), on the other hand, also increased locomotor activity without having any effect on pallidal GABA. These data, taken together, indicate that, while the locomotor effects of dopamine agonists are dependent upon intact mesoaccumbens dopamine and involve GABAergic efferents from the nucleus accumbens to the ventral pallidum, dizocilpine maleate's stimulatory actions are independent of such mechanisms. (C) 1997 IBRO. Published by Elsevier Science Ltd. C1 NIMH,BIOL PSYCHIAT BRANCH,BETHESDA,MD 20892. RP Mele, A (reprint author), UNIV ROMA LA SAPIENZA,DIPARTIMENTO GENET & BIOL MOL,PLE A MORO 5,I-00185 ROME,ITALY. RI Mele, Andrea/E-7741-2015 NR 68 TC 50 Z9 50 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PD JAN PY 1998 VL 82 IS 1 BP 43 EP 58 PG 16 WC Neurosciences SC Neurosciences & Neurology GA YC491 UT WOS:A1998YC49100005 PM 9483502 ER PT J AU Schafer, M Zhou, L Stein, C AF Schafer, M Zhou, L Stein, C TI Cholecystokinin inhibits peripheral opioid analgesia in inflamed tissue SO NEUROSCIENCE LA English DT Article DE opioids; cholecystokinin; peripheral antinociception; CCK receptors; protein kinase C; primary afferent neuron ID DORSAL-ROOT GANGLIA; PROTEIN-KINASE-C; SPINAL-CORD; INSITU HYBRIDIZATION; OCTAPEPTIDE REVERSES; RECEPTOR-BINDING; SENSORY NEURONS; CALCIUM CURRENT; MESSENGER-RNAS; RAT AB There is abundant evidence that opioid receptors are present on peripheral terminals of primary afferent neurons. Experimental and clinical studies have shown that activation of these peripheral opioid receptors produces potent analgesia. In addition to peripheral opioid receptors, cholecystokinin receptors are present in sensory neurons. We examined the hypothesis that cholecystokinin receptors may be present on the same primary afferent neuron and that either exogenous or endogenous cholecystokinin may modulate peripheral antinociceptive effects of mu-opioid receptor agonists. Administration of cholecystokinin into inflamed paws, of the rat, but not intravenously attenuated peripheral antinociceptive effects induced by two mu-opioid receptor agonists, [D-Ala(2),N-methyl-Phe(4),Gly-ol(5)]-enkephalin and fentanyl. Only the desulphated form of cholecystokinin produced significant and dose-dependent attenuation. Cholecystokinin alone did not alter nociceptive baseline values in inflamed or non-inflamed paws. The anti-opioid effect of cholecystokinin was dose-dependently antagonized by the cholecystokinin(B) receptor-selective antagonist L-365260, but not by the cholecystokinin, receptor-selective antagonist L-364718. Local pretreatment with the protein kinase C specific inhibitor calphostin C abolished cholecystokinin's effect. Peripheral antinociceptive effects of [D-Ala(2),N-methyl-Phe(4),Gly-ol(5)]-enkephalin and fentanyl were not altered by intraplantar L-365260 alone. These results indicate that activation of peripheral cholecystokinin(B) but not cholecystokinin(A) receptors attenuates the local antinociceptive effects of mu-opioid receptor agonists in inflamed tissue. This anti-opioid effect may be mediated by protein kinase C in sensory nerve terminals. Endogenous cholecystokinin does not seem to influence the efficacy of peripheral opioids under both normal and inflammatory conditions. (C) 1997 IBRO. Published by Elsevier Science Ltd. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT ANESTHESIOL & CRIT CARE MED,BALTIMORE,MD 21287. RP Schafer, M (reprint author), NIDA,BEHAV PHARMACOL & GENET SECT,DIV INTRAMURAL RES,BALTIMORE,MD 21224, USA. FU NINDS NIH HHS [R01NS32466] NR 49 TC 27 Z9 28 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PD JAN PY 1998 VL 82 IS 2 BP 603 EP 611 PG 9 WC Neurosciences SC Neurosciences & Neurology GA YE569 UT WOS:A1998YE56900020 PM 9466464 ER PT J AU McDonald, MP Overmier, JB AF McDonald, MP Overmier, JB TI Present imperfect: A critical review of animal models of the mnemonic impairments in Alzheimer's disease SO NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS LA English DT Review DE animal models; Alzheimer's disease; memory; hippocampus; medial septum; nucleus basalis magnocellularis; fimbria-fornix; scopolamine ID NUCLEUS BASALIS MAGNOCELLULARIS; SHORT-TERM-MEMORY; MEDIAL SEPTAL-LESIONS; NICTITATING-MEMBRANE RESPONSE; FIMBRIA-FORNIX LESIONS; AMYLOID-BETA-PROTEIN; MATCHING-TO-SAMPLE; RADIAL-ARM MAZE; FOREBRAIN CHOLINERGIC SYSTEM; IBOTENIC-ACID LESIONS AB This paper reviews the current literature on animal models of the memory impairments of Alzheimer's disease (AD). The authors suggest that modeling of the mnemonic deficits in AD be limited to the amnesia observed early in the course of the disease, to eliminate the influence of impairments in non-mnemonic processes. Tasks should be chosen for their specificity and selectivity to the behavioral phenomena observed in early-stage AD and not for their relevance to hypothetical mnemonic processes. Tasks that manipulate the delay between learning and remembering are better able to differentiate Alzheimer patients from persons with other disorders, and better able to differentiate' effects of manipulations in animals. The most commonly used manipulations that attempt to model the amnesia of AD are reviewed within these constraints. The authors conclude that of the models examined, lesions of the medial septal nucleus produce behavioral deficits that are most similar to the mnemonic impairments in the earliest stage of AD. However, the parallel is not definitive and more work is needed to clarify the relationship between neurobiology and behavior in AD. (C) 1998 Elsevier Science Ltd. All rights reserved. C1 Univ Minnesota, Dept Psychol, Minneapolis, MN 55455 USA. RP McDonald, MP (reprint author), NIMH, Room 4N214,Bldg 10, Bethesda, MD 20892 USA. EM mikemc@codon.nih.gov NR 315 TC 56 Z9 59 U1 5 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0149-7634 J9 NEUROSCI BIOBEHAV R JI Neurosci. Biobehav. Rev. PD JAN PY 1998 VL 22 IS 1 BP 99 EP 120 PG 22 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA YW578 UT WOS:000071950400006 PM 9491942 ER PT J AU Brenneman, DE Hauser, J Phillips, T Festoff, B Gozes, I AF Brenneman, DE Hauser, J Phillips, T Festoff, B Gozes, I TI Neuron-glial interactions that regulate CNS development and provide neuroprotection SO NEUROSCIENCE LETTERS LA English DT Meeting Abstract C1 NICHD, Sect Dev & Mol Pharmacol, Bethesda, MD 20892 USA. George Washington Univ, Med Ctr, Immunochem Lab, Washington, DC 20037 USA. Univ Kansas, Med Ctr, Kansas City, MO USA. Tel Aviv Univ, Sackler Sch Med, Dept Clin Biochem, IL-69978 Ramat Aviv, Israel. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PY 1998 SU 51 BP S7 EP S7 PG 1 WC Neurosciences SC Neurosciences & Neurology GA 147RT UT WOS:000077512200030 ER PT J AU Gozes, I Zamostiano, R Pinhasov, A Steingart, R Giladi, E Brenneman, DE AF Gozes, I Zamostiano, R Pinhasov, A Steingart, R Giladi, E Brenneman, DE TI New discoveries for a VIP-associated stress-defense cycle SO NEUROSCIENCE LETTERS LA English DT Meeting Abstract C1 Tel Aviv Univ, Sackler Sch Med, Dept Clin Biochem, IL-69978 Ramat Aviv, Israel. NICHD, Sect Dev & Mol Pharmacol, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PY 1998 SU 51 BP S17 EP S17 PG 1 WC Neurosciences SC Neurosciences & Neurology GA 147RT UT WOS:000077512200070 ER PT J AU Lomnitski, L Nyska, A Shohami, E Chen, Y Michaelson, DM AF Lomnitski, L Nyska, A Shohami, E Chen, Y Michaelson, DM TI Elevated levels of cellular iron in apoE-deficient mouse drain following closed head injury SO NEUROSCIENCE LETTERS LA English DT Meeting Abstract C1 Tel Aviv Univ, Dept Neurobiochem, IL-69978 Tel Aviv, Israel. NIEHS, Res Triangle Pk, NC 27709 USA. Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Pharmacol, IL-91902 Jerusalem, Israel. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PY 1998 SU 51 BP S27 EP S27 PG 1 WC Neurosciences SC Neurosciences & Neurology GA 147RT UT WOS:000077512200115 ER PT J AU Meiri, N Sun, MK Segal, Z Dahl, D Tomsic, D Cavallaro, S Alkon, DL AF Meiri, N Sun, MK Segal, Z Dahl, D Tomsic, D Cavallaro, S Alkon, DL TI Memory and LTP dissociated: Antisense approach to study neuronal plasticity SO NEUROSCIENCE LETTERS LA English DT Meeting Abstract C1 Agr Res Org, Volcani Ctr, Inst Anim Sci, Dept Poultry Sci, IL-50250 Bait Dagan, Israel. NIH, Lab Adapt Syst, Bethesda, MD 20892 USA. RI yu, yan/C-2322-2012; Cavallaro, Sebastiano/F-3104-2010 OI Cavallaro, Sebastiano/0000-0001-7590-1792 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PY 1998 SU 51 BP S29 EP S29 PG 1 WC Neurosciences SC Neurosciences & Neurology GA 147RT UT WOS:000077512200124 ER PT J AU Offen, D Panet, H Sharki, Y Fridkin, M Brenneman, DE Melamed, E Gozes, I AF Offen, D Panet, H Sharki, Y Fridkin, M Brenneman, DE Melamed, E Gozes, I TI VIP protects against 6-hydroxydopamine toxicity: Implications for treatment of Parkinson's disease SO NEUROSCIENCE LETTERS LA English DT Meeting Abstract C1 Rabin Med Ctr, Dept Neurol, IL-49100 Petah Tiqwa, Israel. Rabin Med Ctr, FMRC, IL-49100 Petah Tiqwa, Israel. Weizmann Inst Sci, Dept Organ Chem, IL-76100 Rehovot, Israel. NICHD, Sect Dev & Mol Pharmacol, Bethesda, MD 20892 USA. Tel Aviv Univ, Sackler Sch Med, Gildor Chair, IL-69978 Tel Aviv, Israel. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PY 1998 SU 51 BP S30 EP S30 PG 1 WC Neurosciences SC Neurosciences & Neurology GA 147RT UT WOS:000077512200130 ER PT J AU Pinhasov, A Giladi, E Bassan, M Fridkin, M Brenneman, DE Gozes, I AF Pinhasov, A Giladi, E Bassan, M Fridkin, M Brenneman, DE Gozes, I TI Activity-dependent neurotrophic factor: Femtomolar-acting peptides interact to provide neuroprotection in Alzheimer's models in vivo SO NEUROSCIENCE LETTERS LA English DT Meeting Abstract C1 Tel Aviv Univ, Sackler Sch Med, IL-69978 Ramat Aviv, Israel. Weizmann Inst Sci, Dept Organ Chem, IL-76100 Rehovot, Israel. NICHD, Sect Dev & Mol Pharmacol, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PY 1998 SU 51 BP S33 EP S33 PG 1 WC Neurosciences SC Neurosciences & Neurology GA 147RT UT WOS:000077512200142 ER PT J AU Royak, Y Mandel, S Bick, T Jacobson, KJ Pinchasi, B Youdim, MBH AF Royak, Y Mandel, S Bick, T Jacobson, KJ Pinchasi, B Youdim, MBH TI The influence of an adenosine receptor A(2A) antagonist on lipid peroxidation, cell death induced by 6-hydroxydopamine and H2O2, and calcium influx, in PC12 cells and SH-SY5Y human neuroblastoma cells SO NEUROSCIENCE LETTERS LA English DT Meeting Abstract C1 Technion Israel Inst Technol, Fac Med, Eve Topf & US Natl Parkinsons Fdn Ctr Neurodegene, IL-31096 Haifa, Israel. NIDDK, Mol Recognit Sect, LBC, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PY 1998 SU 51 BP S35 EP S35 PG 1 WC Neurosciences SC Neurosciences & Neurology GA 147RT UT WOS:000077512200152 ER PT J AU Zarchin, N Meilin, S Mendelman, A Levinger, U Rifkind, J Mayevsky, A AF Zarchin, N Meilin, S Mendelman, A Levinger, U Rifkind, J Mayevsky, A TI Age-related responses of the rat cortex to hypoxia and hypercapnia SO NEUROSCIENCE LETTERS LA English DT Meeting Abstract C1 Bar Ilan Univ, Dept Life Sci, IL-52900 Ramat Gan, Israel. Rabin Med Ctr, Dept B, IL-49100 Petah Tiqwa, Israel. NIA, LCMB, Gerontol Res Ctr, NIH, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PY 1998 SU 51 BP S46 EP S46 PG 1 WC Neurosciences SC Neurosciences & Neurology GA 147RT UT WOS:000077512200199 ER PT J AU Fields, RD AF Fields, RD TI Cell adhesion molecules: Implications for neurological disease SO NEUROSCIENTIST LA English DT Article ID MYELIN-ASSOCIATED GLYCOPROTEIN; X-LINKED HYDROCEPHALUS; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; CENTRAL-NERVOUS-SYSTEM; CORPUS-CALLOSUM; SPINAL-CORD; ADULT-RATS; NEUROMUSCULAR-JUNCTION; RECOGNITION MOLECULES; RECESSIVE INHERITANCE C1 NICHD, Dev Neurobiol Lab, NIH, Neurocytol & Physiol Unit, Bethesda, MD 20892 USA. EM fields@helix.nih.gov NR 70 TC 10 Z9 10 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 1073-8584 J9 NEUROSCIENTIST JI Neuroscientist PD JAN PY 1998 VL 4 IS 1 BP 4 EP 8 DI 10.1177/107385849800400107 PG 5 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA YW409 UT WOS:000071932400007 ER PT J AU Koch, CA Moore, JL Voth, D AF Koch, CA Moore, JL Voth, D TI Arachnoid cysts: how do postsurgical cyst size and seizure outcome correlate? SO NEUROSURGICAL REVIEW LA English DT Article; Proceedings Paper CT 11th International Congress of Neurological Surgery CY JUL 06-11, 1997 CL AMSTERDAM, NETHERLANDS DE arachnoid cyst; cyst fenestration; cystoperitoneal shunt; cyst size; epilepsy surgery; seizures ID MIDDLE CRANIAL FOSSA; TEMPORAL-LOBE EPILEPSY; SPONTANEOUS DISAPPEARANCE; SURGICAL-TREATMENT; HEAD-INJURY; CHILDREN; DIAGNOSIS; ADULTS; BRAIN; SHUNT AB Arachnoid cysts (ACs) are congenital cystic brain malformations associated with epilepsy. The purpose of this study was to determine the effect of surgical intervention of ACs on cyst size and seizure outcome. We reviewed the world's medical literature dealing with surgically treated ACs in epilepsy patients. Our stud; included only cases, in which the relationship between pre- and postoperative CT-size of the AC and seizure outcome was described. We also included six patients with ACs and epilepsy treated surgically at the University of Mainz. We analyzed postoperative AC size and seizure outcome with respect to mode of operation, cyst location, and patients' age. A total of 76 patients was reviewed. Sixty (79 %) patients had a smaller AC postoperatively. Forty-six of those 60 (76.6 %) experienced seizure improvement. Thirteen patients (21.6 %)remained unchanged and one patient (1.8 %) worsened. In 16 of the 76 patients (21 %) the postoperative AC size was unchanged. Eight of those 16 patients improved. Six patients (37.5 %) remained unchanged and two (12.5 %) worsened. A positive correlation between postoperative AC size and seizure outcome was well demonstrated among patients treated by cystfenestration, needle aspiration, or internal shunting. Among patients treated by cystoperitoneal shunting this direct correlation was less clear. Seizure outcome correlates directly with postoperative AC size. Seizure reduction is associated with decreased AC size postoperatively and depends on the mode of operation. Based on these data we would expect that patients with epilepsy secondary to ACs would demonstrate improved seizure control with lower AC volume. Conversely, we might expect increasing AC size to correlate with worse seizure control. This relationship may guide physicians in efficacy and timely patient management. C1 Ohio State Univ Hosp, Dept Med, Columbus, OH 43210 USA. Ohio State Univ Hosp, Dept Neurol, Columbus, OH 43210 USA. Univ Mainz, Dept Neurosurg, D-6500 Mainz, Germany. RP Koch, CA (reprint author), NICHD, NIH, Bldg 10,Room 10 N 262, Bethesda, MD 20892 USA. RI Koch, Christian/A-4699-2008; OI Koch, Christian/0000-0003-3127-5739; Koch, Christian/0000-0003-0678-1242 NR 66 TC 27 Z9 28 U1 5 U2 6 PU WALTER DE GRUYTER & CO PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0344-5607 J9 NEUROSURG REV JI Neurosurg. Rev. PY 1998 VL 21 IS 1 BP 14 EP 22 DI 10.1007/BF01111480 PG 9 WC Clinical Neurology; Surgery SC Neurosciences & Neurology; Surgery GA 107UU UT WOS:000075230000003 PM 9584281 ER PT J AU Koch, CA Bornstein, SR Pacak, K Chrousos, GP AF Koch, CA Bornstein, SR Pacak, K Chrousos, GP TI How does metyrapone decrease seizures? SO NEUROSURGICAL REVIEW LA English DT Letter ID CORTICOTROPIN-RELEASING FACTOR; INFANTILE SPASMS; BRAIN; CRF; RAT C1 NICHD, DEB, NIH, Bethesda, MD 20892 USA. RP Koch, CA (reprint author), NICHD, DEB, NIH, Bldg 10,Room 10 N 262, Bethesda, MD 20892 USA. RI Koch, Christian/A-4699-2008; OI Koch, Christian/0000-0003-3127-5739; Koch, Christian/0000-0003-0678-1242 NR 10 TC 1 Z9 1 U1 1 U2 1 PU WALTER DE GRUYTER & CO PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0344-5607 J9 NEUROSURG REV JI Neurosurg. Rev. PY 1998 VL 21 IS 4 BP 302 EP 303 DI 10.1007/BF01105791 PG 2 WC Clinical Neurology; Surgery SC Neurosciences & Neurology; Surgery GA 168WH UT WOS:000078715800017 PM 10068196 ER PT J AU Nelson, N Liu, QR AF Nelson, Nathan Liu, Qing-Rong TI Cloning of genes or cDNAs encoding neurotransmitter transporters and their localization by immunocytochemistry SO NEUROTRANSMITTER TRANSPORTERS SE METHODS IN ENZYMOLOGY LA English DT Review ID DEVELOPMENTAL EXPRESSION; MOUSE-BRAIN; GABA TRANSPORTER; GLYT2; GAT4 C1 Tel Aviv Univ, Dept Biochem, IL-69978 Tel Aviv, Israel. NIDA, Mol Neurobiol Branch, Baltimore, MD 21224 USA. RP Nelson, N (reprint author), Tel Aviv Univ, Dept Biochem, IL-69978 Tel Aviv, Israel. RI Liu, Qing-Rong/A-3059-2012 OI Liu, Qing-Rong/0000-0001-8477-6452 NR 12 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1998 VL 296 BP 52 EP 64 PG 13 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BGJ33 UT WOS:000247400300003 PM 9779439 ER PT J AU Vaughan, RA AF Vaughan, Roxanne A. TI Cocaine and GBR Photoaffinity labels as probes of dopamine transporter structure SO NEUROTRANSMITTER TRANSPORTERS SE METHODS IN ENZYMOLOGY LA English DT Review ID LIGAND-BINDING DOMAINS; NOREPINEPHRINE TRANSPORTERS; PHOSPHORYLATION; SUBSTRATE; SITES C1 NIDA, Mol Neurobiol Branch, Intramural Res Program, Baltimore, MD 21224 USA. RP Vaughan, RA (reprint author), NIDA, Mol Neurobiol Branch, Intramural Res Program, Baltimore, MD 21224 USA. NR 17 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1998 VL 296 BP 219 EP 230 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BGJ33 UT WOS:000247400300015 PM 9779451 ER PT J AU Clark, JA AF Clark, Janet A. TI Analysis of transporter topology using deletion and epitope tagging SO NEUROTRANSMITTER TRANSPORTERS SE METHODS IN ENZYMOLOGY LA English DT Review ID AMINOBUTYRIC-ACID TRANSPORTER; MEMBRANE-SPANNING REGIONS; HUMAN P-GLYCOPROTEIN; TRANSMEMBRANE TOPOLOGY; ENDOPLASMIC-RETICULUM; XENOPUS OOCYTES; GABA TRANSPORTER; FUSION PROTEINS; MESSENGER-RNA; ALPHA-SUBUNIT C1 NIMH, Genet Sect, Bethesda, MD 20892 USA. RP Clark, JA (reprint author), NIMH, Genet Sect, Bethesda, MD 20892 USA. NR 33 TC 2 Z9 2 U1 0 U2 1 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1998 VL 296 BP 293 EP 307 PG 15 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BGJ33 UT WOS:000247400300020 PM 9779456 ER PT J AU Uhl, G Lin, ZC Metzger, T Dar, DE AF Uhl, George Lin, Zhicheng Metzger, Thomas Dar, Dalit E. TI Dopamine transporter mutants, small molecules, and approaches to cocaine antagonist/dopamine transporter disinhibitor development SO NEUROTRANSMITTER TRANSPORTERS SE METHODS IN ENZYMOLOGY LA English DT Review ID RAT-BRAIN; EXPRESSION; CLONING; BINDING; RECEPTOR; CDNA C1 Johns Hopkins Univ, Sch Med, Mol Neurobiol Branch, NIDA IRP, Baltimore, MD 21224 USA. Johns Hopkins Univ, Sch Med, Mol Neurobiol Branch, Dept Neurol, Baltimore, MD 21224 USA. Johns Hopkins Univ, Sch Med, Mol Neurobiol Branch, Dept Neurosci, Baltimore, MD 21224 USA. RP Uhl, G (reprint author), Johns Hopkins Univ, Sch Med, Mol Neurobiol Branch, NIDA IRP, Baltimore, MD 21224 USA. NR 33 TC 19 Z9 19 U1 1 U2 1 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1998 VL 296 BP 456 EP 465 PG 10 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BGJ33 UT WOS:000247400300031 PM 9779467 ER PT B AU Kaga, M Inagaki, M Uno, A AF Kaga, M Inagaki, M Uno, A BE Perat, MV TI Event related potentials in children with learning disabilities. Visual event related potentials in specific Kanji writing disabilities SO NEW DEVELOPMENTS IN CHILD NEUROLOGY LA English DT Proceedings Paper CT VIII International Child Neurology Congress CY SEP 13-17, 1998 CL LJUBLJANA, SLOVENIA AB Event related potentials in two patients with learning disability in specific 'Kanji' writing were investigated using newly made tasks of familiar simple Kanji, unfamiliar complex Kanji and meaningless complex figures. These tasks are made with full consideration of their clinical neuropsycological symptoms. These patients showed different patterns of abnormalities in these tasks compared with control subjects. These findings suggested the different underlying mechanism of the same clinical symptoms and are helpful to consider strategy of their education and training. C1 NIMH, Dept Dev Disorders, Natl Ctr Neurol & Psychiat, Bethesda, MD 20892 USA. RP Kaga, M (reprint author), NIMH, Dept Dev Disorders, Natl Ctr Neurol & Psychiat, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU MEDIMOND S R L PI 40128 BOLOGNA PA VIA MASERATI 5, 40128 BOLOGNA, 00000, ITALY BN 88-323-0913-0 PY 1998 BP 627 EP 633 PG 7 WC Clinical Neurology; Pediatrics SC Neurosciences & Neurology; Pediatrics GA BL78W UT WOS:000076740500108 ER PT B AU Hirtz, D AF Hirtz, D BE Perat, MV TI Clinical trials in pediatric neurology SO NEW DEVELOPMENTS IN CHILD NEUROLOGY LA English DT Proceedings Paper CT VIII International Child Neurology Congress CY SEP 13-17, 1998 CL LJUBLJANA, SLOVENIA C1 NINDS, Div Fundamental Neurosci & Dev Disorders, Bethesda, MD 20892 USA. RP Hirtz, D (reprint author), NINDS, Div Fundamental Neurosci & Dev Disorders, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MEDIMOND S R L PI 40128 BOLOGNA PA VIA MASERATI 5, 40128 BOLOGNA, 00000, ITALY BN 88-323-0913-0 PY 1998 BP 707 EP 710 PG 4 WC Clinical Neurology; Pediatrics SC Neurosciences & Neurology; Pediatrics GA BL78W UT WOS:000076740500122 ER PT J AU Ioannidis, JPA Cappelleri, JC Lau, J AF Ioannidis, JPA Cappelleri, JC Lau, J TI Meta-analyses and large randomized, controlled trials SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 NIAID, Bethesda, MD 20892 USA. Pfizer Inc, Cent Res, Groton, CT 06340 USA. New England Med Ctr Hosp, Boston, MA 02111 USA. RP Ioannidis, JPA (reprint author), NIAID, 9000 Rockville Pike, Bethesda, MD 20892 USA. RI Ioannidis, John/G-9836-2011 NR 6 TC 27 Z9 28 U1 0 U2 2 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 USA SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 1 PY 1998 VL 338 IS 1 BP 59 EP 59 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA YN801 UT WOS:000071209200012 PM 9424563 ER PT B AU Smith, MA AF Smith, MA BE Levy, AL Grauer, E BenNathan, D Kloet, ER TI The role of brain-derived neurotrophic factor in the central effects of stress SO NEW FRONTIERS IN STRESS RESEARCH: MODULATION OF BRAIN FUNCTION LA English DT Proceedings Paper CT 40th OHOLO Research Conference CY MAR 20-25, 1996 CL ZICHRON YAKOV, ISRAEL SP Commiss European Communities ID SYNAPTIC TRANSMISSION; HIPPOCAMPAL-NEURONS; PYRAMIDAL NEURONS; MESSENGER-RNAS; RAT-BRAIN; EXPRESSION; BDNF; ATROPHY C1 NIMH, Biol Psychiat Branch, Bethesda, MD 20892 USA. RP Smith, MA (reprint author), NIMH, Biol Psychiat Branch, Bethesda, MD 20892 USA. NR 21 TC 1 Z9 1 U1 0 U2 0 PU HARWOOD ACADEMIC PUBL GMBH PI CHUR PA POSTSTRASSE 22, 7000 CHUR, SWITZERLAND BN 90-5702-266-4 PY 1998 BP 53 EP 57 PG 5 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BM36U UT WOS:000078505600005 ER PT S AU Tomer, KB Trojak, SJ Parker, CE AF Tomer, KB Trojak, SJ Parker, CE BE Ens, W Standing, KG Chernushevich, IV TI Structural studies of protein-protein interactions using proteolytic footprinting and MALDI/MS SO NEW METHODS FOR THE STUDY OF BIOMOLECULAR COMPLEXES SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES C, MATHEMATICAL AND PHYSICAL SCIENCES LA English DT Proceedings Paper CT NATO Advanced Research Workshop on New Methods for the Study of Molecular Aggregates CY JUN 16-20, 1996 CL LODGE KANANASKIS VILLAGE, KANANASKIS, CANADA SP NATO, Univ Manitoba, Dept Phys HO LODGE KANANASKIS VILLAGE C1 NIEHS, Mass Spectrometry Grp, Struct Biol Lab, Res Triangle Pk, NC 27709 USA. RP Tomer, KB (reprint author), NIEHS, Mass Spectrometry Grp, Struct Biol Lab, POB 12233, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0258-2023 BN 0-7923-5003-0 J9 NATO ADV SCI I C-MAT PY 1998 VL 510 BP 59 EP 65 PG 7 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Chemistry, Analytical; Chemistry, Physical SC Biochemistry & Molecular Biology; Biophysics; Chemistry GA BK69N UT WOS:000073108600004 ER PT S AU Lecchi, P Pannell, LK AF Lecchi, P Pannell, LK BE Ens, W Standing, KG Chernushevich, IV TI The detection of non-covalent interactions in nucleic acids using MALDI SO NEW METHODS FOR THE STUDY OF BIOMOLECULAR COMPLEXES SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES C, MATHEMATICAL AND PHYSICAL SCIENCES LA English DT Proceedings Paper CT NATO Advanced Research Workshop on New Methods for the Study of Molecular Aggregates CY JUN 16-20, 1996 CL LODGE KANANASKIS VILLAGE, KANANASKIS, CANADA SP NATO, Univ Manitoba, Dept Phys HO LODGE KANANASKIS VILLAGE C1 NIDDKD, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. RP Lecchi, P (reprint author), NIDDKD, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU SPRINGER PI DORDRECHT PA PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0258-2023 BN 0-7923-5003-0 J9 NATO ADV SCI I C-MAT PY 1998 VL 510 BP 217 EP 224 PG 8 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Chemistry, Analytical; Chemistry, Physical SC Biochemistry & Molecular Biology; Biophysics; Chemistry GA BK69N UT WOS:000073108600018 ER PT J AU Krupinski, J Vodovotz, Y Li, CG Slowik, A Beevers, D Flanders, KC Lip, G Kumar, P Szczudlik, A AF Krupinski, J Vodovotz, Y Li, CG Slowik, A Beevers, D Flanders, KC Lip, G Kumar, P Szczudlik, A TI Inducible nitric oxide production and expression of transforming growth factor-beta 1 in serum and CSF after cerebral ischaemic stroke in man SO NITRIC OXIDE-BIOLOGY AND CHEMISTRY LA English DT Article DE stroke; nitric oxide; TGF-beta 1 ID FACTOR-BETA; ALZHEIMERS-DISEASE; SYNTHASE ACTIVITY; GENE-EXPRESSION; ENDOTHELIAL-CELLS; RABBIT MODEL; ISCHEMIA; NEURONS; GROWTH-FACTOR-BETA-1; TGF-BETA-1 AB A residual blood supply to the ischaemic brain is a crucial determinant for tissue survival, Early changes in the vascular network and subsequent angiogenesis may be mediated by short-lived molecules Like nitric oxide (NO) or growth factors such as transforming growth factor-beta 1 (TGF-beta 1). Although TGF-beta 1 can inhibit NO production, this interaction has not been studied after ischaemia in humans. Serum samples were taken from patients at 24 h and 6 months and cerebrospinal fluid (CSF) samples at 24 h and 1 week later for possible correlation between the two factors. Tissue expression of TGF-beta 1 and of the inducible isoform of NO synthase (NOS2) was assessed by immunohistochemistry. CSF levels of NO2-/NO3- as well as total (active + latent) TGF-beta 1 were higher in stroke patients as compared to controls 24 h after the stroke. Both NO2-/NO3- and TGF-beta 1 were lower 6 months after the stroke compared to 24 h, Levels of NO2-/NO3- correlated with levels of TGF-beta 1 within the time points (P = 0.041, Kendall correlation coefficient). There was a strong staining for NOS2 in brain tissue sections in neurones, reactive astrocytes, infiltrating white blood cells, and endothelial cells of larger microvessels. TGF-beta 1 expression was mainly limited to neurones and reactive astrocytes. These findings suggest that the interaction between TGF-beta 1 and NOS2 might be important for angiogenesis after cerebral ischaemia and may indicate that TGF-beta 1 is upregulated as a negative feedback response to elevated levels of NO. (C) 1998 Academic Press. C1 Jagiellonian Univ, Inst Neurol, Dept Neurol, PL-31503 Krakow, Poland. Manchester Metropolitan Univ, Dept Biol Sci, Manchester M15 6BH, Lancs, England. Cardiol Res Fdn, Washington, DC USA. Medlant Res Inst, Washington, DC USA. Univ Manchester, Sch Med, Dept Pathol, Manchester, Lancs, England. NCI, Chemoprevent Lab, Bethesda, MD 20892 USA. Univ Birmingham, City Hosp, Dept Med, Birmingham, W Midlands, England. RP Krupinski, J (reprint author), Jagiellonian Univ, Inst Neurol, Dept Neurol, Ul Bot 3, PL-31503 Krakow, Poland. NR 62 TC 29 Z9 32 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1089-8603 J9 NITRIC OXIDE-BIOL CH JI Nitric Oxide-Biol. Chem. PY 1998 VL 2 IS 6 BP 442 EP 453 DI 10.1006/niox.1998.0204 PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 196HZ UT WOS:000080304100003 PM 10342487 ER PT S AU Chen, YD Dougherty, ER AF Chen, YD Dougherty, ER BE Dougherty, ER Astola, JT TI Queueing interpretation of adaptive reconstructive multiparameter tau-opening filters SO NONLINEAR IMAGE PROCESSING IX SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT Conference on Nonlinear Image Processing IX CY JAN 26-27, 1998 CL SAN JOSE, CA SP Soc Photo Opt Instrumentat Engineers, Soc Imaging Sci & Technol DE Mathematical Morphology; opening; adaptive filter; Markov chain AB A multiparameter binary tau-opening is a union of parameterized openings in which parameters for each opening are individually defined and a structuring element can be parameterized relative to both size and shape. The reconstructive filter corresponding to an opening is defined by fully passing any grain not eliminated by the opening and deleting all other grains. Adaptive design results from treating the parameter vector of a reconstructive multiparameter tau-opening as the state space of a Markov chain. The present paper considers the relationship between Markovian queueing networks and adaptive multiparameter tau-openings for the signal-union-noise model. C1 Natl Human Genome Res Inst, NIH, Bethesda, MD 20892 USA. RP Chen, YD (reprint author), Natl Human Genome Res Inst, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPIE-INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA SN 0277-786X BN 0-8194-2744-6 J9 P SOC PHOTO-OPT INS PY 1998 VL 3304 BP 59 EP 65 DI 10.1117/12.304616 PG 7 WC Engineering, Electrical & Electronic; Optics SC Engineering; Optics GA BK88W UT WOS:000073808200006 ER PT B AU Martin, MA AF Martin, MA GP ORG ECON COOPERAT & DEV ORG ECON COOPERAT & DEV TI The construction and use of HIV-1 transgenic mice in AIDS research SO NOVEL SYSTEMS FOR THE STUDY OF HUMAN DISEASE: FROM BASIC RESEARCH TO APPLICATIONS SE OECD PROCEEDINGS LA English DT Proceedings Paper CT OECD Rome 96 Workshop on Novel Systems for the Study of Human Disease - From Basic Research to Applications CY DEC 09-11, 1996 CL ROME, ITALY ID HUMAN-IMMUNODEFICIENCY-VIRUS; LONG TERMINAL REPEAT; NERVOUS-SYSTEM; TAT GENE; EXPRESSION; INFECTION; DISEASE; MOUSE; CELLS C1 NIAID, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA. RP Martin, MA (reprint author), NIAID, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA. NR 18 TC 0 Z9 0 U1 0 U2 0 PU ORGANIZATION ECONOMIC COOPERATION & DEVELOPMENT PI PARIS PA 2, RUE ANDRE PASCAL, CEDEX 16, 75775 PARIS, FRANCE BN 92-64-16011-6 J9 OECD PROC PY 1998 BP 209 EP 217 PG 9 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Research & Experimental Medicine GA BN25V UT WOS:000081339500018 ER PT J AU Benson, DA Boguski, MS Lipman, DJ Ostell, J Ouellette, BFF AF Benson, DA Boguski, MS Lipman, DJ Ostell, J Ouellette, BFF TI GenBank SO NUCLEIC ACIDS RESEARCH LA English DT Article ID HUMAN GENOME; MAP AB The GenBank (R) sequence database (http://www.ncbi.nlm.nih.gov/) incorporates DNA sequences from all available public sources, primarily through the direct submission of sequence data from individual laboratories and from large-scale sequencing projects, Most submitters use the Banklt (WWW) or Sequin programs to send their sequence data, Data exchange with the EMBL Data Library and the DNA Data Bank of Japan helps ensure comprehensive worldwide coverage. GenBank data is accessible through NCBI's integrated retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome and protein structure information, MEDLINE (R) abstracts from published articles describing the sequences are also included as an additional source of biological annotation, Sequence similarity searching is offered through the BLAST series of database search programs. In addition to FTP, e-mail and server/client versions of Entrez and BLAST, NCBI offers a wide range of World Wide Web retrieval and analysis services of interest to biologists. C1 Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. RP Benson, DA (reprint author), Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bldg 38A,8600 Rockville Pike, Bethesda, MD 20894 USA. NR 11 TC 328 Z9 332 U1 0 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 1 PY 1998 VL 26 IS 1 BP 1 EP 7 DI 10.1093/nar/26.1.1 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA YV004 UT WOS:000071778900001 PM 9399790 ER PT J AU Hainaut, P Hernandez, T Robinson, A Rodriguez-Tome, P Flores, T Hollstein, M Harris, CC Montesano, R AF Hainaut, P Hernandez, T Robinson, A Rodriguez-Tome, P Flores, T Hollstein, M Harris, CC Montesano, R TI IARC Database of p53 gene mutations in human tumors and cell lines: updated compilation, revised formats and new visualisation tools SO NUCLEIC ACIDS RESEARCH LA English DT Article ID SUPPRESSOR GENE; CANCER PATIENTS; TRANSACTIVATION; PATHOGENESIS; ETIOLOGY; SOFTWARE; MUTANTS AB Since 1989 about 570 different p53 mutations have been identified in more than 8000 human cancers. A database of these mutations was initiated by M. Hollstein and C. C. Harris in 1990. This database originally consisted of a list of somatic point mutations in the p53 gene of human tumors and cell lines, compiled from the published literature and made available in a standard electronic form, The database is maintained at the International Agency for Research on Cancer (IARC) and updated versions are released twice a year (January and July). The current version (July 1997) contains records on 6800 published mutations and will surpass the 8000 mark in the January 1998 release, The database now contains information on somatic and germline mutations in a new format to facilitate data retrieval, In addition, new tools are constructed to improve data analysis, such as a Mutation Viewer Java applet developed at the European Bioinformatics Institute (EBI) to visualise the location and impact of mutations on p53 protein structure, The database is available in different electronic formats at IARC (http://www.iarc.fr/p53/homepage.htm) or from the EBI server (http://www.ebi.ac.uk). The IARC p53 website also provides reports on database analysis and links with other p53 sites as well as with related databases, In this report, we describe the criteria for inclusion of data, the revised format and the new visualisation tools. We also briefly discuss the relevance of p53 mutations to clinical and biological questions. C1 Int Agcy Res Canc, F-69372 Lyon 08, France. European Bioinformat Inst, EMBL, Outstn, Cambridge, England. German Canc Res Ctr, D-69120 Heidelberg, Germany. NCI, Human Carcinogenesis Lab, Bethesda, MD 20892 USA. RP Hainaut, P (reprint author), Int Agcy Res Canc, 150 Cours Albert Thomas, F-69372 Lyon 08, France. RI Robinson, Alan/A-9956-2009; Hernandez, Tonia/J-9335-2012; Hainaut, Pierre /B-6018-2012 OI Hainaut, Pierre /0000-0002-1303-1610 FU Wellcome Trust NR 30 TC 365 Z9 374 U1 2 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 1 PY 1998 VL 26 IS 1 BP 205 EP 213 DI 10.1093/nar/26.1.205 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA YV004 UT WOS:000071778900049 PM 9399837 ER PT J AU Martinez, E Moore, DD Keller, E Pearce, D Vanden Heuvel, JP Robinson, V Gottlieb, B MacDonald, P Simons, S Sanchez, E Danielsen, M AF Martinez, E Moore, DD Keller, E Pearce, D Vanden Heuvel, JP Robinson, V Gottlieb, B MacDonald, P Simons, S Sanchez, E Danielsen, M TI The Nuclear Receptor Resource: a growing family SO NUCLEIC ACIDS RESEARCH LA English DT Article AB Last year, the original Glucocorticoid Receptor Resource expanded into a comprehensive project: the Nuclear Receptor Resource (NRR, http://nrr.georgetown.edu/nrr/nrr.html). The NRR has since been offering comprehensive information on nuclear receptor structure and function, as well as general facts of interest to the scientific community on meetings, funding and employment opportunities, The project now includes individual resources as part of a network which integrates information on glucocorticoid, androgen, mineralocorticoid, thyroid hormone, Vitamin D and peroxisome-proliferator activated receptors. Many investigators have joined the NRR network by filling out the Who is who? form available in the NRR home page. This has facilitated communication among scientists in the field and dissemination of data not otherwise published. Because several investigators have contacted NRR authors over the past few months asking for advice and materials for educational purposes, we have recently decided to include in our project an educational resource on nuclear receptors termed the 'Graphics Library'. The input and suggestions of NRR users do shape the future direction of the project, so we encourage users to continue to give us feedback. C1 Georgetown Univ, Sch Med, Dept Biochem & Mol Biol, Washington, DC 20007 USA. Texas Med Ctr, Dept Cell Biol, Houston, TX 77030 USA. Eastern Virginia Med Sch, Glennan Ctr, Norfolk, VA 23507 USA. Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA. Penn State Univ, Dept Vet Sci, University Pk, PA 16802 USA. J David Gladstone Inst, San Francisco, CA 94141 USA. Sir Mortimer B Davis Jewish Hosp, Lady Davis Inst Med Res, Quebec City, PQ H3T 1E2, Canada. St Louis Univ, Hlth Sci Ctr, Dept Pharmacol & Physiol Sci, St Louis, MO 63104 USA. NIDDK, Steroid Hormones Sect, NIH, Bethesda, MD 20892 USA. Med Coll Ohio, Dept Pharmacol, Toledo, OH 43699 USA. RP Danielsen, M (reprint author), Georgetown Univ, Sch Med, Dept Biochem & Mol Biol, 3900 Reservoir Rd NW, Washington, DC 20007 USA. RI Danielsen, Mark/B-1606-2008 OI Danielsen, Mark/0000-0002-0923-9945 FU PHS HHS [K04 02105] NR 0 TC 17 Z9 17 U1 0 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 1 PY 1998 VL 26 IS 1 BP 239 EP 241 DI 10.1093/nar/26.1.239 PG 3 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA YV004 UT WOS:000071778900056 PM 9471621 ER PT J AU Sonnhammer, ELL Eddy, SR Birney, E Bateman, A Durbin, R AF Sonnhammer, ELL Eddy, SR Birney, E Bateman, A Durbin, R TI Pfam: multiple sequence alignments and HMM-profiles of protein domains SO NUCLEIC ACIDS RESEARCH LA English DT Article ID HIDDEN MARKOV-MODELS; HOMOLOGY AB Pfam contains multiple alignments and hidden Markov model based profiles (HMM-profiles) of complete protein domains. The definition of domain boundaries, family members and alignment is done semi-automatically based on expert knowledge, sequence similarity, other protein family databases and the ability of HMM-profiles to correctly identify and align the members, Release 2.0 of Pfam contains 527 manually verified families which are available for browsing and on-line searching via the World Wide Web in the UK at http://www.sanger.ac.uk/Pfam/ and in the US at http://genome.wustl.edu/Pfam/Pfam 2.0 matches one or more domains in 50% of Swissprot-34 sequences, and 25% of a large sample of predicted proteins from the Caenorhabditis elegans genome. C1 Natl Lib Med, Natl Ctr Biotechnol Informat, Computat Biol Branch, Bethesda, MD 20894 USA. Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA. Sanger Ctr, Cambridge CB10 1SA, England. RP Sonnhammer, ELL (reprint author), Natl Lib Med, Natl Ctr Biotechnol Informat, Computat Biol Branch, Bldg 38A,Room 8N805, Bethesda, MD 20894 USA. RI Bateman, Alex/E-6518-2011; OI Bateman, Alex/0000-0002-6982-4660 FU NHGRI NIH HHS [R01-HG01363]; Wellcome Trust NR 14 TC 422 Z9 439 U1 1 U2 13 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 1 PY 1998 VL 26 IS 1 BP 320 EP 322 DI 10.1093/nar/26.1.320 PG 3 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA YV004 UT WOS:000071778900077 PM 9399864 ER PT J AU Baxevanis, AD Landsman, D AF Baxevanis, AD Landsman, D TI Histone Sequence Database: new histone fold family members SO NUCLEIC ACIDS RESEARCH LA English DT Article ID PROTEIN; CHROMATIN; ALIGNMENT; DNA; MOTIF; TOOL AB Searches of the major public protein databases with core and linker chicken and human histone sequences have resulted in the compilation of an annotated set of histone protein sequences, In addition, new database searches with two distinct motif search algorithms have identified several members of the histone fold family, including human DRAP1 and yeast CSE4. Database resources include information on conflicts between similar sequence entries in different source databases, multiple sequence alignments, links to the Entrez integrated information retrieval system, structures for histone and histone fold proteins, and the ability to visualize structural data through Cn3D. The database currently contains >1000 protein sequences, which are searchable by protein type, accession number, organism name, or any other free text appearing in the definition line of the entry, All sequences and alignments in this database are available through the World Wide Web at http://www.nhgri.nih.gov/DIR/GTB/HISTONES or http://www.ncbi.nlm.nih.gov/Baxevani/HISTONES. C1 NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. NIH, Genome Technol Branch, Natl Ctr Human Genome Res, Bethesda, MD 20892 USA. RP Landsman, D (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. RI Landsman, David/C-5923-2009; OI Landsman, David/0000-0002-9819-6675 NR 29 TC 29 Z9 29 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 1 PY 1998 VL 26 IS 1 BP 372 EP 375 DI 10.1093/nar/26.1.372 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA YV004 UT WOS:000071778900090 PM 9399877 ER PT J AU Marquez, VE Ezzitouni, A Russ, P Siddiqui, MA Ford, H Feldman, RJ Mitsuya, H George, C Barchi, JJ AF Marquez, VE Ezzitouni, A Russ, P Siddiqui, MA Ford, H Feldman, RJ Mitsuya, H George, C Barchi, JJ TI Lessons from the pseudorotational cycle: Conformationally rigid AZT carbocyclic nucleosides and their interaction with reverse transcriptase SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article; Proceedings Paper CT International Conference on Nucleic Acids and Related Macromolecules: Synthesis, Structure, and Application CY SEP 04-09, 1997 CL ULM, GERMANY ID THYMIDINE; OLIGONUCLEOTIDES AB Two conformationally locked carba-AZT nucleoside 5'-triphosphates built on a rigid bicyclo[3.1.0]hexane template showed exclusive Northern (E-2) and Southern (E-3) conformations, respectively. Inhibition of reverse transcriptase (RT) occurred selectively with the Northern carba-AZT triphosphate. C1 NCI, Med Chem Lab, Div Basic Sci, NIH, Bethesda, MD 20892 USA. NCI, Expt Retrovirol Sect, Med Branch, Div Clin Sci,NIH, Bethesda, MD 20892 USA. USN, Res Lab, Struct Matter Lab, Washington, DC 20375 USA. RP Marquez, VE (reprint author), NCI, Med Chem Lab, Div Basic Sci, NIH, Bethesda, MD 20892 USA. RI Barchi Jr., Joseph/N-3784-2014 NR 11 TC 13 Z9 13 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1998 VL 17 IS 9-11 BP 1881 EP 1884 DI 10.1080/07328319808004726 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 130VF UT WOS:000076540500046 ER PT J AU Kitade, Y Wakana, M Terai, S Tsuboi, T Nakanishi, M Yatome, C Dong, BH Silverman, RH Torrence, PF AF Kitade, Y Wakana, M Terai, S Tsuboi, T Nakanishi, M Yatome, C Dong, BH Silverman, RH Torrence, PF TI 2-bromoadenosine-substituted 2 ',5 '-oligoadenylates modulate binding and activation abilities of human recombinant RNase 1 SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article ID BIOLOGICAL-ACTIVITIES; 2-5A-DEPENDENT RNASE; ANALOGS; 2-5A; BASE AB 2-Bromoadenosine-substituted analogues of 2-5A, p5'A2'p-5'A2'p5'(br(2)A), p5'(br(2)A)2'p5'A2'p5'A, and p5'(br(2)A)2'p5'(br(2)A)2'p5'(br(2)A), were prepared via a modification of a lead ion-catalyzed ligation reaction and were subsequently converted into the corresponding 5'-triphosphates. Both binding and activation of human recombinant RNase L by various 2-bromoadenosine-substituted 2-5A analogues were examined. Among the 2-bromoadenosine-substituted 2-5A analogues, the analogue with 2-bromoadenosine residing in the 2'-terminal position, p5'A2'p5'A2'p5'(br(2)A), showed the strongest binding affinity and was as effective as 2-5A itself as an activator of RNase L. The CD spectrum of p5'A2'p5'A2'p5'(br(2)A) was superimposable on that of p5'A2'p5'A2'p5'A, indicative of an anti orientation about the base-glycoside bonds as in naturally occurring 2-5A. C1 Gifu Univ, Dept Biomol Sci, Lab Mol Biochem, Fac Engn, Gifu 5011193, Japan. Cleveland Clin Fdn, Lerner Res Inst, Dept Canc Biol, Cleveland, OH 44195 USA. NIDDKD, Med Chem Lab, Sect Biomed Chem, NIH, Bethesda, MD 20892 USA. RP Kitade, Y (reprint author), Gifu Univ, Dept Biomol Sci, Lab Mol Biochem, Fac Engn, Yanagido 1-1, Gifu 5011193, Japan. NR 18 TC 6 Z9 6 U1 0 U2 2 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1998 VL 17 IS 12 BP 2323 EP 2333 DI 10.1080/07328319808004320 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 143AF UT WOS:000077181300014 ER PT J AU Hageman, GJ Stierum, RH van Herwijnen, MHM van der Veer, MSE Kleinjans, JCS AF Hageman, GJ Stierum, RH van Herwijnen, MHM van der Veer, MSE Kleinjans, JCS TI Nicotinic acid supplementation: Effects on niacin status, cytogenetic damage, and poly(ADP-ribosylation) in lymphocytes of smokers SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Article ID DNA STRAND-BREAKS; SISTER CHROMATID EXCHANGES; ADENINE-DINUCLEOTIDE; POLYMERASE-ACTIVITY; ADP-RIBOSYLATION; REPAIR; BLOOD; DEFICIENCY; INVIVO; RIBOSE AB As a substrate for poly(ADP-ribose) polymerase (PARP; EC, 2.4.2.30), an enzyme that is activated by DNA strand breaks and is thought to facilitate efficient DNA repair, NAD(+) and its precursor nicotinic acid (niacin) are involved in the cellular defense against DNA damage by genotoxic compounds. In this study, the effect of nicotinic acid supplementation on cytogenetic damage and poly(ADP-ribosylation) was evaluated in a human population that is continuously exposed to genotoxic agents, e.g., smokers. By use of a placebo-controlled intervention design, 21 healthy smokers received supplementary nicotinic acid at 0-100 mg/day for 14 weeks. An increased niacin status, as assessed from blood nicotinamide concentrations and lymphocyte NAD(+) concentrations, was observed in groups supplemented with 50 and 100 mg/day. This effect was most pronounced in subjects with lower initial NAD(+) levels. An increased niacin status did not result in decreased hypoxanthine guanine phosphoribosyltransferase variant frequencies and micronuclei induction in peripheral blood lymphocytes (PBLs). Sister chromatid exchanges in PBLs, however, were increased after supplementation with nicotinic acid. This increase was positively associated with the daily dose of nicotinic acid. No effects of nicotinic acid supplementation were found for ex vivo (+/-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7, 7,8,9,10-tetrahydrobenzo[a]pyrene-induced poly(ADP-ribosylation), although the small number of samples that could be analyzed (n = 12) does not allow firm; conclusions. Because no evidence was found far a decrease in cigarette smoke-induced cytogenetic damage in PBLs of smokers after nicotinic acid supplementation of up to 100 mg/day, it is concluded that supplemental niacin does not contribute to a reduced genetic risk in healthy smokers. C1 Univ Maastricht, Dept Hlth Risk Anal & Toxicol, NL-6200 MD Maastricht, Netherlands. NIA, Mol Genet Lab, Baltimore, MD 21224 USA. RP Hageman, GJ (reprint author), Univ Maastricht, Dept Hlth Risk Anal & Toxicol, POB 616, NL-6200 MD Maastricht, Netherlands. RI Hageman, Geja/B-5298-2013; Kleinjans, Jos/E-7241-2015 NR 25 TC 11 Z9 11 U1 0 U2 1 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 USA SN 0163-5581 J9 NUTR CANCER JI Nutr. Cancer PY 1998 VL 32 IS 2 BP 113 EP 120 PG 8 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA 157KE UT WOS:000078059400010 PM 9919621 ER PT J AU Malila, N Virtanen, M Pietinen, P Virtamo, J Albanes, D Hartman, AM Heinonen, OP AF Malila, N Virtanen, M Pietinen, P Virtamo, J Albanes, D Hartman, AM Heinonen, OP TI A comparison of prospective and retrospective assessments of diet in a study of colorectal cancer SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Article ID COLON-CANCER; DAIRY-PRODUCTS; BREAST-CANCER; RECALL BIAS; WOMENS HEALTH; RISK-FACTORS; FAT INTAKE; MEAT; QUESTIONNAIRE; VEGETABLES AB Dietary factors are widely studied as risk factors for colorectal cancer, with much information from case-control studies. We evaluated the validity of dietary data from a retrospective case-control study of diet and colorectal cancer As part of the alpha-Tocopherol, beta-Carotene Cancer Prevention Study. diet was assessed at baseline and after diagnosis for colorectal cancer cases and at baseline and regularly during the trial for a random control group. The dietary assessment referred to the previous 12 months (in cases before diagnosis). In the two dietary assessments, the cases reported a greater increase in consumption of fruits and dairy products and a decrease in consumption of potatoes. Accordingly, relative risks for colorectal cancer by baseline dietary data differed markedly from odds ratios from case-control data; e.g., relative risk for a 652-mg increase in calcium intake was 0.79 (95% confidence interval = 0.48-1.30) in case-cohort analysis vs. an odds ratio of 1.57 (95% confidence interval = 1.06-2.33) for case-control analysis. The most likely explanation is the influence of current diet on recall of prediagnosis diet and effects of occult cancer on diet in the year before cancer diagnosis, which have implications fbr interpretation of case-control studies in evaluating associations between diet and colorectal cancer. C1 Natl Publ Hlth Inst, Helsinki, Finland. NCI, Bethesda, MD 20892 USA. Univ Helsinki, Dept Publ Hlth, Helsinki, Finland. RP Malila, N (reprint author), Natl Publ Hlth Inst, Mannerheimintie 166, Helsinki, Finland. RI Albanes, Demetrius/B-9749-2015 FU NCI NIH HHS [N01-CN-45165] NR 46 TC 10 Z9 11 U1 0 U2 1 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 USA SN 0163-5581 J9 NUTR CANCER JI Nutr. Cancer PY 1998 VL 32 IS 3 BP 146 EP 153 PG 8 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA 165FX UT WOS:000078511000004 PM 10050264 ER PT J AU Hartman, TJ Tangrea, JA Pietinen, P Malila, N Virtanen, M Taylor, PR Albanes, D AF Hartman, TJ Tangrea, JA Pietinen, P Malila, N Virtanen, M Taylor, PR Albanes, D TI Tea and coffee consumption and risk of colon and rectal cancer in middle-aged Finnish men SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Article ID DIRECT-ACTING MUTAGENICITY; LARGE-BOWEL CANCER; COLORECTAL-CANCER; PROSPECTIVE COHORT; DIETARY HABITS; FOOD GROUPS; BLACK TEA; MORTALITY; PHENOLICS; ALCOHOL AB The association between coffee and black tea consumption and the subsequent risk of colon and rectal cancer was investigated within a Finnish clinical trial cohort. One hundred eleven cases of colon cancer and 83 cases of rectal cancer were diagnosed over a median of 8.0 years of follow-up. Proportional hazards regression models were used to derive adjusted relative risks (RR) and 95% confidence intervals (CI) for the association between coffee and tea consumption and cancer incidence. After controlling for confounders, coffee was not significantly associated with colon or rectal cancer. A positive association was seen for increased consumption of tea drinking and colon cancer. Compared with persons who did not drink tea those who consumed < 1 cup/day had an RR of 1.40 (95% CI = 0.84-2.33) and those who consumed greater than or equal to 1 cup/day had an RR of 2. 09 (95% CI = 1.34-3.26 p for trend = 0.001). In contrast, tea consumption had little effect on rectal cancer incidence. This study does not support the hypothesis that coffee and tea prefect against colorectal cancer risk. However, given the strength of the tea-colon cancer association and the significant gradient of risk we observed across level of intake, further epidemiologic research of this relationship in other populations seems warranted. C1 NCI, Div Clin Sci, Bethesda, MD 20892 USA. Natl Publ Hlth Inst, Helsinki, Finland. RP Hartman, TJ (reprint author), NCI, Div Clin Sci, Bethesda, MD 20892 USA. RI Albanes, Demetrius/B-9749-2015 FU NCI NIH HHS [N01-CN-45165] NR 56 TC 55 Z9 55 U1 1 U2 4 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 USA SN 0163-5581 J9 NUTR CANCER JI Nutr. Cancer PY 1998 VL 31 IS 1 BP 41 EP 48 PG 8 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA 102BN UT WOS:000074903500006 PM 9682247 ER PT J AU Snyderwine, EG Thorgeirsson, UP Venugopal, M Roberts-Thomson, SJ AF Snyderwine, EG Thorgeirsson, UP Venugopal, M Roberts-Thomson, SJ TI Mammary gland carcinogenicity of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in Sprague-Dawley rats on high- and low-fat diets SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Article ID CANCER CELL-LINE; BREAST-CANCER; HETEROCYCLIC AMINES; LINOLEIC-ACID; INVASIVE CAPACITY; COOKED FOODS; NUDE-MICE; IN-VITRO; RISK; TUMORIGENESIS AB 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a carcinogenic heterocyclic amine derived from cooked meat. Mammary gland tumors were induced in female Sprague-Dawley rats given 10 doses of PhIP (75 mg/kg po) once per day from 43 days of age and then placed on a defined high-fat (23.5% corn oil) or low-fat (5% corn oil) diet for 25 weeks. Mammary tumor incidence was 49% (44 of 90 rats) and 31% (27 of 88 rats) in the high- and low-fat groups, respectively. No tumors were found in vehicle control rats on the high- or the low-fat diet (n = 44 and 43, respectively). The higher tumor incidence in the high-fat group was due to an increase specifically in carcinomas (classified as tubulopapillary carcinomas) rather than benign tumors (tubular adenomas and fibroadenomas). The incidence of carcinomas was 45% and 24% in PhIP-treated rats on the high- and low-fat diets, respectively. In addition, the percentage of carcinomas showing stromal invasion was highest in the high-fat diet group (22% vs. 8%, high- vs. low-fat diet). Proliferating cell nuclear antigen immunostaining (PCNA) index revealed six times more proliferation in carcinomas from rats on the high-fat diet than in rats on the low-fat diet. Adenomas from rats on different diets had similar PCNA indexes. The tumor apoptotic index, quantitated by immunohistochemical detection (terminal deoxynucleotidyl transferase nick end labeling), was twice as high in carcinomas from rats on the high-fat diet as in carcinomas from rats on the low-fat diet but was similar between the two groups of adenomas. The PCNA-to-apoptosis ratio was 43 and 17 in carcinomas from rats on the high- and low-fat diets, respectively, indicating that the growth rate of carcinomas was greater in rats on the high-fat diet. The results from this study show that the high-fat diet increases the incidence, invasiveness, and growth of PhIP-induced mammary gland carcinomas. C1 NIH, Chem Carcinogenesis Sect, Expt Carcinogenesis Lab, Bethesda, MD 20892 USA. NIH, Div Basic Sci, Cellular Carcinogenesis & Tumor Promot Lab, Bethesda, MD 20892 USA. RP Snyderwine, EG (reprint author), NCI, Expt Carcinogenesis Lab, Chem Carcinogenesis Sect, Bldg 37,Rm 3C28, Bethesda, MD 20892 USA. RI Roberts-Thomson, Sarah/B-4282-2011 OI Roberts-Thomson, Sarah/0000-0001-8202-5786 NR 52 TC 44 Z9 44 U1 0 U2 2 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 USA SN 0163-5581 J9 NUTR CANCER JI Nutr. Cancer PY 1998 VL 31 IS 3 BP 160 EP 167 PG 8 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA 128HF UT WOS:000076399400002 PM 9795967 ER PT J AU Breslow, RA Weed, DL AF Breslow, RA Weed, DL TI Review of epidemiologic studies of alcohol and prostate cancer: 1971-1996 SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Review ID RISK-FACTORS; UNITED-STATES; ARTICLES; CONSUMPTION; SMOKING; DIET; MEN; POPULATION; COHORT; CALIFORNIA AB Prostate cancer is the most common cancer among American men, with few established risk factors. The association between prostate cancer and alcohol, a potentially modifiable risk factor, has been examined in numerous studies. We systematically reviewed the literature on alcohol and the incidence of prostate cancer by searching for published cohort and case-control studies using computerized databases, references, and experts, by evaluating studies for validity, and by summarizing the results and providing research recommendations. We found compelling evidence for no association between low-to-moderate alcohol consumption and prostate cancer. Most studies, however did nor assess the risk of heavy drinking, where there has been some suggestion of increased risk, or of lifetime patterns of drinking. None of the studies have used genetic markers, nor have they been conducted in populations with known familial risk. Further studies in some populations may be warranted. C1 NCI, Div Canc Prevent & Control, Bethesda, MD 20892 USA. RP Breslow, RA (reprint author), NCI, Div Canc Prevent & Control, EPN 313, Bethesda, MD 20892 USA. NR 54 TC 36 Z9 36 U1 0 U2 1 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 USA SN 0163-5581 J9 NUTR CANCER JI Nutr. Cancer PY 1998 VL 30 IS 1 BP 1 EP 13 PG 13 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA YY310 UT WOS:000072134300001 PM 9507506 ER PT J AU Wideroff, L Potischman, N Glass, AG Greer, CE Manos, MM Scott, DR Burk, RD Sherman, ME Wacholder, S Schiffman, M AF Wideroff, L Potischman, N Glass, AG Greer, CE Manos, MM Scott, DR Burk, RD Sherman, ME Wacholder, S Schiffman, M TI A nested case-control study of dietary factors and the risk of incident cytological abnormalities of the cervix SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Article ID INTRAEPITHELIAL NEOPLASIA; VITAMIN-A; CANCER; FOLATE AB Several earlier case-control studies reported inverse associations of cervical squamous intraepithelial lesions (SIL) with high dietary or biomarker levels of carotenoids, folate, and vitamins C and E. However, most studies did not measure the primary causal factor, cancer-associated genital human papillomaviruses (HPV), now detected by sensitive viral DNA tests. This nested case-control study assessed whether high dietary intakes of these nutrients, plus zinc and vitamin A, reduced SIL risk in cancer-associated HPV DNA-positive women. Using a 60-item food-frequency questionnaire, nutrient estimates were obtained for 33 incident cases with high-grade lesions, 121 with low-grade lesions, 97 with equivocal SIL, and 806 cytologically normal controls sampled from a large prospective cohort study. Baseline cervicovaginal lavages were tested for HPV DNA by the polymerase chain reaction. Among DNA-positive cases (n = 68) and controls (n = 69), age-adjusted odds ratios (ORs) of SIL in the highest vs, the lowest nutrient quartiles were 1.4 [95% confidence interval (CI) = 0.5-4.2] for vitamin A, 0.6 (CI = 0.2-2.0) for beta-carotene, 1.3 (CI = 0.4-3.6) for vitamin C, 1.0 (CI = 0.4-3.6) for vitamin E, 0.7 (CI = 0.3-2.1) for folate, and 0.8 (CI = 0.3-2.2) for zinc. ORs in HPV DNA-negative women approximated 1.0, with the exception of vitamin E (OR = 0.5 CI = 0.3-0.9). These results do not support a protective role for the above nutrients against low-grade or equivocal SIL, which constituted the majority of diagnoses in this study. C1 NCI, DCCPS, ARB, Bethesda, MD 20892 USA. NCI, Epidemiol & Biostat Program, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. Kaiser Permanente Ctr Hlth Res, Portland, OR 97227 USA. Chiron Corp, Emeryville, CA 94608 USA. Kaiser Res Inst, Oakland, CA 94611 USA. Yeshiva Univ Albert Einstein Coll Med, Bronx, NY 10461 USA. Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA. RP Wideroff, L (reprint author), NCI, DCCPS, ARB, EPN 313,MSC 7344,6130 Execut Bl, Bethesda, MD 20892 USA. RI Hernandez, Jessica/G-6527-2011 NR 27 TC 20 Z9 21 U1 0 U2 2 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 USA SN 0163-5581 J9 NUTR CANCER JI Nutr. Cancer PY 1998 VL 30 IS 2 BP 130 EP 136 PG 7 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA ZK223 UT WOS:000073297700006 PM 9589431 ER PT J AU Rumsaeng, V Metcalfe, DD AF Rumsaeng, V Metcalfe, DD TI Asthma and food allergy SO NUTRITION REVIEWS LA English DT Article; Proceedings Paper CT Conference on Nutrition and Immunity CY MAY 05-07, 1997 CL ATLANTA, GEORGIA SP Emory Univ Sch Med, ILSI Res Fdn, Ctr Dis Control & Prevent, Amer Canc Soc, Amer Soc Nutr Sci, FAO, ILSI Europe, ILSI N Amer, WHO ID EXERCISE-INDUCED ANAPHYLAXIS; CROSS-REACTIVE ALLERGEN; IN-HOUSE DUST; ATOPIC-DERMATITIS; DOUBLE-BLIND; MONOSODIUM GLUTAMATE; IGE; SHRIMP; MITE; HYPERSENSITIVITY C1 NIAID, Bethesda, MD 20892 USA. RP Rumsaeng, V (reprint author), NIAID, Bethesda, MD 20892 USA. NR 72 TC 3 Z9 3 U1 0 U2 1 PU INT LIFE SCIENCES INST PI LAWRENCE PA 810 EAST 10TH ST SUBSCRIPTION OFFICE, LAWRENCE, KS 66044 USA SN 0029-6643 J9 NUTR REV JI Nutr. Rev. PD JAN PY 1998 VL 56 IS 1 SU S BP S153 EP S160 PN 2 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA YW310 UT WOS:000071921100022 PM 9481138 ER PT J AU Anasti, JN Kalantaridou, SN Kimzey, LM Defensor, RA Nelson, LM AF Anasti, JN Kalantaridou, SN Kimzey, LM Defensor, RA Nelson, LM TI Bone loss in young women with karyotypically normal spontaneous premature ovarian failure SO OBSTETRICS AND GYNECOLOGY LA English DT Article ID POSTMENOPAUSAL WOMEN; ORAL ESTROGENS; PREMENOPAUSAL; DENSITY; MENOPAUSE; SPINE; FEMUR; MASS AB Objective: To evaluate the effects of karotypically normal spontaneous premature ovarian failure on femoral neck bone mineral density. Methods: Eighty-nine women with karyotypically normal spontaneous premature ovarian failure who desired fertility were evaluated at a tertiary care academic center and underwent hip and spinal bone density measurements by conventional dual-photon absorptiometry. Seventy-seven of the women (87%) had sought medical advice previously and had taken a variety of estrogen and progestin replacement regimens at least intermittently. The median (range) age was 32 (20-39) years, and the median (range) time since diagnosis was 1.5 (0.5-11) years. Findings were compared with a reference group of 218 regularly menstruating women of similar age. Results: Sixty of the 89 women with premature ovarian failure (67%, 95% confidence interval 57, 77) had a femoral neck bone mineral density more than 1 standard deviation (SD) below the mean of the reference group (P < .001, chi(2) with Yates correction). Even in women in whom the bone mineral density measurement was made within just 1.5 years of the diagnosis, nearly one-half (47%) had a femoral neck bone mineral density more than 1 SD below the mean of the reference group (P < .01). Conclusion: Two-thirds of young women with karyotypically normal spontaneous premature ovarian failure have a femoral neck bone mineral density more than 1 SD below the mean of a reference group. These young women need early education regarding strategies to maintain their bone mass and ongoing medical evaluation to maintain compliance with these strategies. C1 NICHHD, Sect Womens Hlth, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA. NIH, Ctr Clin, Dept Nursing, Bethesda, MD 20892 USA. RP Nelson, LM (reprint author), NICHHD, Sect Womens Hlth, Dev Endocrinol Branch, NIH, Bldg 10,Room 10N262, Bethesda, MD 20892 USA. NR 20 TC 74 Z9 77 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD JAN PY 1998 VL 91 IS 1 BP 12 EP 15 DI 10.1016/S0029-7844(97)00583-8 PG 4 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA YP061 UT WOS:000071237300003 PM 9464713 ER PT J AU Kogevinas, M Sala, M Boffetta, P Kazerouni, N Kromhout, H Hoar-Zahm, S AF Kogevinas, M Sala, M Boffetta, P Kazerouni, N Kromhout, H Hoar-Zahm, S TI Cancer risk in the rubber industry: a review of the recent epidemiological evidence SO OCCUPATIONAL AND ENVIRONMENTAL MEDICINE LA English DT Review ID NON-HODGKINS-LYMPHOMA; CERTIFICATE CASE-CONTROL; LOWER URINARY-TRACT; BLADDER-CANCER; LUNG-CANCER; OCCUPATIONAL EXPOSURES; DEATH-CERTIFICATE; MULTIPLE-MYELOMA; CASE-REFERENT; LYMPHOCYTIC-LEUKEMIA AB Objectives-To examine the recent epidemiological evidence on cancer risk among workers in the rubber industry. Methods-Epidemiological studies published after the last detailed review by the International Agency for Research on Cancer (IARC) in 1982 were reviewed. 12 cohort studies in nine countries that examined distinct populations of workers in the rubber industry, seven industry based nested case-control studies, 48 community based case-control studies in 16 countries, and 23 studies based on administrative data that reported risks for employment in the rubber industry were identified. Results-Excess risks of bladder cancer, lung cancer, and leukaemia were found in most studies, with risks above 1.5 in about half of the studies. A moderate excess risk for laryngeal cancer was consistent across studies. Excess risks were found in a few studies for cancers of the oesophagus, stomach, colon, liver, pancreas, skin, prostate, kidney, brain, and thyroid, and for malignant lymphoma and multiple myeloma, but overall results were not consistent for these neoplasms. Conclusions-Magnitude of the observed risks varied considerably between studies, but overall the findings indicate the presence of a widespread moderate increased cancer risk among rubber workers. The most consistent results were for bladder, laryngeal, and lung cancer and for leukaemia. Excess risks were also found for other neoplasms but an evaluation of the consistency of the findings is difficult because of the possible selective reporting of results. Recent studies do not provide information associating specific exposures With cancer risk. The preventive measures taken in the rubber industry in recent years may decrease risks, but this has not been documented yet in epidemiological studies. C1 Inst Municipal Invest Med, Resp & Environm Hlth Res Unit, E-08003 Barcelona, Spain. Int Agcy Res Canc, Unit Environm Canc Epidemiol, F-69372 Lyon, France. NCI, Occupat Epidemiol Branch, Bethesda, MD USA. Wageningen Univ Agr, Dept Environm & Occupat Hlth, Wageningen, Netherlands. RP Kogevinas, M (reprint author), Inst Municipal Invest Med, Resp & Environm Hlth Res Unit, 80 Doctor Aiguader Rd, E-08003 Barcelona, Spain. EM KOGEVINAS@IMIM.ES RI Kromhout, Hans/A-9159-2008; sala, maria/A-2593-2011; Zahm, Shelia/B-5025-2015; Fernandez , Irene/E-5705-2016; Martinez, Esther/H-2562-2013; Kogevinas, Manolis/C-3918-2017; OI Sala Serra, Maria/0000-0002-5516-5967 NR 119 TC 99 Z9 103 U1 1 U2 4 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 1351-0711 J9 OCCUP ENVIRON MED JI Occup. Environ. Med. PD JAN PY 1998 VL 55 IS 1 BP 1 EP 12 PG 12 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA YQ824 UT WOS:000071426800001 PM 9536156 ER PT J AU Farrow, A Shea, KM Little, RE AF Farrow, A Shea, KM Little, RE CA ALSPAC Study Team TI Birthweight of term infants and maternal occupation in a prospective cohort of pregnant women SO OCCUPATIONAL AND ENVIRONMENTAL MEDICINE LA English DT Article DE birthweight; maternal occupation; prospective study ID LOW-BIRTH-WEIGHT; GESTATIONAL-AGE; PRETERM DELIVERY; SPONTANEOUS-ABORTIONS; WORK; OUTCOMES; INDUSTRY; ASSISTANTS; EMPLOYMENT; EXPOSURES AB Objective-To study the relation between birthweight of term infants and maternal occupation. Methods-Information on job titles since the age of 16, and sociodemographic and other lifestyle factors were obtained by means of questionnaires as part of the Avon longitudinal study of pregnancy and childhood (ALSPAC), from a cohort of 14 000 pregnant women. The British 1990 standard occupational classification was used to code jobs within nine major job groups. Results-For 9282 women who delivered term infants and reported a job for the relevant period, there was a significant difference in mean birthweight among the nine major job groups. A 148 g difference was found between the mean birthweight of infants born to women with professional occupations and those with plant and machine operative jobs. Multiple regression analysis adjusted for sex of infant, parity, maternal height, smoking, caffeine consumption, and race. After adjustment the maternal job was no longer significantly associated with birthweight. Conclusion-Despite the absence of a significant association between birthweight and job after adjustment, there were several findings which agreed with publications on maternal occupation and pregnancy outcome. The major job groups with the lowest birthweights included the following jobs: metal forming or welding, electric or electronic work, jobs in the textile trade, and assembling and working with equipment (mobile and stationary). The lack of an association may indicate that the study was of insufficient power to detect a small difference; it may indicate the presence of confounding variables that were not adjusted for or it may indicate that no association exists. C1 Univ Bristol, Inst Child Hlth, Bristol BS8 1TH, Avon, England. NIEHS, Res Triangle Pk, NC 27709 USA. RP Farrow, A (reprint author), Brunel Univ, Dept Hlth Studies, Borough Rd, Isleworth TW7 5DU, Middx, England. FU Wellcome Trust NR 37 TC 8 Z9 9 U1 2 U2 2 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 1351-0711 J9 OCCUP ENVIRON MED JI Occup. Environ. Med. PD JAN PY 1998 VL 55 IS 1 BP 18 EP 23 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA YQ824 UT WOS:000071426800003 PM 9536158 ER PT J AU Petralia, SA Vena, JE Freudenheim, JL Marshall, JR Michalek, A Brasure, J Swanson, M Graham, S AF Petralia, SA Vena, JE Freudenheim, JL Marshall, JR Michalek, A Brasure, J Swanson, M Graham, S TI Breast cancer risk and lifetime occupational history: employment in professional and managerial occupations SO OCCUPATIONAL AND ENVIRONMENTAL MEDICINE LA English DT Article DE breast cancer; occupation; epidemiology; case-control ID RADIOLOGIC TECHNOLOGISTS; UNITED-STATES; MORTALITY; WOMEN; WORKERS; COHORT; HAIRDRESSERS; INDUSTRY; DENMARK; NURSES AB Objective-In this case-control study, occupational histories were used to assess the relation between risk of breast cancer and employment in professional and managerial occupations while adjusting for reproductive and other risk factors. Methods-Incident, primary, female cases of breast cancer diagnosed between 1986 and 1991, and randomly selected controls were interviewed to obtain detailed medical, reproductive, and occupational histories. Mantel-Haenszel crude odds ratios (OR) and 95% confidence intervals (95% CIs) were used to estimate risk of breast cancer related to the job of longest duration. Unconditional logistic regression was used to estimate crude and adjusted ORs and 95% CIs associated with having ever been employed and duration of employment in a professional or managerial occupation. Results-A non-significant threefold increase in risk was found among premenopausal women whose major job was in the occupational category of precision production, craft, and repair (95% CI 0.90 to 20.35). No increase in risk was found for premenopausal women whose major job was a managerial or professional occupation. However, an inverse relation between risk of premenopausal breast cancer and having ever held a professional or managerial job was observed (OR 0.53, 95% CI 0.34 to 0.82). This relation was strongest for women who worked one to 10 years (OR 0.47, 95% CI 0.29 to 0.77). Postmenopausal breast cancer was not related to professional and managerial employment. Conclusions-In this population, employment in professional and managerial occupations is not associated with postmenopausal risk of breast cancer, but seems to be related to a reduction in risk of premenopausal breast cancer. Methodological limitations of this study including response rates are discussed. C1 SUNY Buffalo, Dept Social & Prevent Med, Buffalo, NY 14260 USA. Arizona Canc Ctr, Tucson, AZ USA. Roswell Pk Canc Inst, Buffalo, NY USA. RP Petralia, SA (reprint author), NCI, Occupat Epidemiol Branch, Execut Plaza N,Rm 418,6130 Execut Blvd, Bethesda, MD 20892 USA. FU NCI NIH HHS [CA-01633, CA09051, CA11535] NR 44 TC 17 Z9 18 U1 5 U2 5 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 1351-0711 J9 OCCUP ENVIRON MED JI Occup. Environ. Med. PD JAN PY 1998 VL 55 IS 1 BP 43 EP 48 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA YQ824 UT WOS:000071426800007 PM 9536162 ER PT S AU Hoffman, HJ Ishii, EK Macturk, RH AF Hoffman, HJ Ishii, EK Macturk, RH BE Murphy, C TI Age-related changes in the prevalence of smell/taste problems among the United States adult population - Results of the 1994 disability supplement to the National Health Interview Survey (NHIS) SO OLFACTION AND TASTE XII: AN INTERNATIONAL SYMPOSIUM SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT International Symposium on Olfaction and Taste XII CY JUL 07-12, 1997 CL SAN DIEGO, CA SP Int Commiss Olfact & Taste, NIH, NIA, NIDOCD, NIDR, NICHHD, Natl Sci Fdn, San Diego Univ, Univ California San Diego, Ajinomoto US Inc, Coca Cola Co, Firmenich SA, Glaxo Wellcome Res & Dev Ltd, Int Flavors & Fragrances Inc, Johnson & Johnson, Kirin Brewery Co Ltd, Nutrisweet Kelco C0, Olfactory Res Fund, Pherin Corp, Proctor & Gamble Co, Abbott Labs, Ross Prod Div, Soc Res Umami Taste, Unilever ID TASTE; SMELL; IDENTIFICATION; SPAN AB Information about the prevalence of disorders of the chemical senses has been limited. In the late 1970s, the consensus among experts convened by the National Institutes of Health (NTH) was that more than 2 million adults in the United States had a disorder of smell or taste. A large, nonrandom survey conducted by the National Geographic Society in 1987 found that 1% of their 1.2 million respondents could not smell 3 or more of 6 odorants using a 'scratch and sniff' test. Age was an important factor, with a decline beginning in the second decade of life. No comparable data have been available for taste, although it has been suggested that the sense of taste remains more robust with age. The National Institute on Deafness and Other Communication Disorders (NIDCD), NM, began collaborating with the National Center for Health Statistics (NCHS) in 1993 to acquire information on the prevalence of smell/taste problems using the Disability Supplement to the National Health Interview Survey (NHIS). This survey was administered to approximately 42,000 randomly-selected households (representing about 80,000 adults over 18 years of age) in 1994. Adjusted national estimates derived from this survey showed a prevalence of 2.7 million (1.4%) U.S. adults with an olfactory problem. Also, 1.1 million (0.6%) adults reported a gustatory problem. When smell or taste problems were combined, 3.2 million (1.65%) adults indicated a chronic chemosensory problem. The prevalence rates increased exponentially with age. Almost 40% with a chemosensory problem (1.5 million) were 65 years of age or greater. In a multivariate analysis, the individual's overall health status, other sensory impairments, functional limitations (including difficulty standing or bending), depression, phobia, and several other health-related characteristics were associated with an increase in the rate of chemosensory disorders. C1 NIDCD, Epidemiol Stat & Data Syst Branch, NIH, Bethesda, MD 20892 USA. RP NIDCD, Epidemiol Stat & Data Syst Branch, NIH, Execut Plaza S,Room 432,6120 Execut Blvd,MSC 7180, Bethesda, MD 20892 USA. EM HoffmanH@ms.nidcd.nih.gov NR 26 TC 102 Z9 103 U1 3 U2 10 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-140-5; 1-57331-139-1 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 855 BP 716 EP 722 DI 10.1111/j.1749-6632.1998.tb10650.x PG 7 WC Biochemistry & Molecular Biology; Food Science & Technology; Multidisciplinary Sciences; Neurosciences; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Food Science & Technology; Science & Technology - Other Topics; Neurosciences & Neurology; Nutrition & Dietetics GA BM29Q UT WOS:000078304300109 PM 9929676 ER PT S AU Guo, SW Shen, FM Wang, YD Zheng, CJ AF Guo, SW Shen, FM Wang, YD Zheng, CJ BE Murphy, C TI Threshold distributions of phenylthiocarbamide (PTC) in the Chinese population SO OLFACTION AND TASTE XII: AN INTERNATIONAL SYMPOSIUM SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT International Symposium on Olfaction and Taste XII CY JUL 07-12, 1997 CL SAN DIEGO, CALIFORNIA SP Int Commiss Olfact & Taste, NIH, NIA, NIDOCD, NIDR, NICHHD, Natl Sci Fdn, San Diego Univ, Univ California San Diego, Ajinomoto US Inc, Coca Cola Co, Firmenich SA, Glaxo Wellcome Res & Dev Ltd, Int Flavors & Fragrances Inc, Johnson & Johnson, Kirin Brewery Co Ltd, Nutrisweet Kelco C0, Olfactory Res Fund, Pherin Corp, Proctor & Gamble Co, Abbott Labs, Ross Prod Div, Soc Res Umami Taste, Unilever ID SPLINE DENSITY-ESTIMATION AB The ability to taste phenylthiocarbamide (PTC) is a well-documented Mendelian trait. Mapping and cloning the gene(s) responsible for the PTC tasting ability would help to delineate the molecular basis for the variations in PTC tasting ability in humans and to shed new light on taste chemosensory functions. In view of the spectacular successes in genome science, the positional cloning strategy seems to be a feasible approach to the isolation of the gene(s) underlying the PTC tasting ability. As a first step toward mapping the gene(s), we collected PTC taste threshold data on 106 individuals, most of them being university students, in Shanghai, China. Using various parametric and nonparametric statistical methods, we have found that the data set is best described by a bimodal distribution. The frequency of PTC nontasters is estimated to be 10%, This is consistent with the view that the PTC nontasting ability follows a recessive mode of inheritance. Several authors had previously reported PTC data on Chinese living outside China. Our data are, to our knowledge, the first ever collected from the Chinese population within China. C1 Univ Minnesota, Div Epidemiol, Sch Publ Hlth, Minneapolis, MN 55454 USA. Univ Minnesota, Inst Human Genet, Minneapolis, MN 55454 USA. Shanghai Med Univ, Dept Epidemiol, Shanghai 200032, Peoples R China. Univ Michigan, Dept Biostat, Ann Arbor, MI 48109 USA. NIDCD, Epidemiol Branch, NIH, Bethesda, MD 20892 USA. RP Guo, SW (reprint author), Univ Minnesota, Div Epidemiol, Sch Publ Hlth, Minneapolis, MN 55454 USA. FU NIGMS NIH HHS [R01-GM56515, R29-GM52205] NR 8 TC 21 Z9 21 U1 0 U2 7 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-139-1 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 855 BP 810 EP 812 DI 10.1111/j.1749-6632.1998.tb10664.x PG 3 WC Biochemistry & Molecular Biology; Food Science & Technology; Multidisciplinary Sciences; Neurosciences; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Food Science & Technology; Science & Technology - Other Topics; Neurosciences & Neurology; Nutrition & Dietetics GA BM29Q UT WOS:000078304300123 PM 9929690 ER PT S AU Zheng, CJ Hoffman, HJ Lucchina, LA Bartoshuk, LM Weiffenbach, JM AF Zheng, CJ Hoffman, HJ Lucchina, LA Bartoshuk, LM Weiffenbach, JM BE Murphy, C TI Comparison of the green scale versus magnitude estimation for taste perception SO OLFACTION AND TASTE XII: AN INTERNATIONAL SYMPOSIUM SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT International Symposium on Olfaction and Taste XII CY JUL 07-12, 1997 CL SAN DIEGO, CALIFORNIA SP Int Commiss Olfact & Taste, NIH, NIA, NIDOCD, NIDR, NICHHD, Natl Sci Fdn, San Diego Univ, Univ California San Diego, Ajinomoto US Inc, Coca Cola Co, Firmenich SA, Glaxo Wellcome Res & Dev Ltd, Int Flavors & Fragrances Inc, Johnson & Johnson, Kirin Brewery Co Ltd, Nutrisweet Kelco C0, Olfactory Res Fund, Pherin Corp, Proctor & Gamble Co, Abbott Labs, Ross Prod Div, Soc Res Umami Taste, Unilever AB The Green scale is a new psychophysical method that is simple for subjects to use, but its relation with magnitude estimation has yet to be fully characterized. In comparing the consistency between the Green scale and magnitude estimation, we found that the former seems to provide a psychological oral sensation measurement that is different from the latter method. A simple correction formula can be derived. C1 NIDC, NIH, Bethesda, MD 20792 USA. Yale Univ, New Haven, CT 06520 USA. NIDR, Bethesda, MD 20892 USA. RP Zheng, CJ (reprint author), NIDCD, Epidemiol Branch, NIH, 3120 Execut Blvd,EPS 432, Bethesda, MD 20892 USA. NR 3 TC 2 Z9 2 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-139-1 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 855 BP 820 EP 822 DI 10.1111/j.1749-6632.1998.tb10667.x PG 3 WC Biochemistry & Molecular Biology; Food Science & Technology; Multidisciplinary Sciences; Neurosciences; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Food Science & Technology; Science & Technology - Other Topics; Neurosciences & Neurology; Nutrition & Dietetics GA BM29Q UT WOS:000078304300126 PM 9929693 ER PT J AU Johnson, BE Kelley, MJ AF Johnson, BE Kelley, MJ TI Autocrine growth factors and neuroendocrine markers in the development of small-cell lung cancer SO ONCOLOGY-NEW YORK LA English DT Article; Proceedings Paper CT Conference of Current Perspectives in the Treatment of Lung Cancer CY AUG 10-12, 1997 CL UNIV COLL DUBLIN, DUBLIN, IRELAND HO UNIV COLL DUBLIN ID ADHESION MOLECULE; MONOCLONAL-ANTIBODY; EXPRESSION AB Two different clinical trials using biological agents directed against an autocrine growth factor and a surface marker of neuroendocrine differentiation have been used for patients with relapsed small-cell lung cancer. In a phase II trial, an antibody (2A11) directed against the autocrine growth factor gastrin-releasing peptide has been used to treat patient with relapsed small-cell lung cancer. One of 12 evaluable patients treated with 2A11 250 mg/m(2) three times weekly for 4 weeks achieved a complete response. An antibody directed against the neural cell adhesion molecule has been linked to a modified ricin molecule. This immunotoxin, N901-bR, has undergone phase I testing, and a recommended phase II dose of 30 mu g/kg/day for 7 days by continuous infusion has been determined. In the phase I trial, one of 21 patients with relapsed or refractory small-cell lung cancer had a partial response to this treatment. Therefore, it appears that an antibody directed against an autocrine growth factor and an immunotoxin directed against a surface marker of neuroendocrine differentiation can inhibit the growth of small-cell lung cancer in vitro and in vivo; both produced some evidence of antitumor activity in patients. Further studies with agents directed against autocrine growth factors and surface markers of neuroendocrine differentiation appear warranted. C1 NCI, Natl Naval Med Ctr, Lung Canc Biol Sect, Med Branch,Div Clin Studies, Bethesda, MD 20889 USA. RP Johnson, BE (reprint author), NCI, Natl Naval Med Ctr, Lung Canc Biol Sect, Med Branch,Div Clin Studies, Bldg 8,Room 5101, Bethesda, MD 20889 USA. OI Kelley, Michael/0000-0001-9523-6080 NR 14 TC 5 Z9 5 U1 0 U2 0 PU P R R INC PI HUNTINGTON PA 17 PROSPECT ST, HUNTINGTON, NY 11743 USA SN 0890-9091 J9 ONCOLOGY-NY JI Oncology-NY PD JAN PY 1998 VL 12 IS 1 SU 2 BP 11 EP 14 PG 4 WC Oncology SC Oncology GA ZC418 UT WOS:000072576000001 PM 9516605 ER PT J AU Takenoshita, S Mogi, A Osawa, H Sunaga, H Kakegawa, H Tani, M Morinaga, N Kato, R Hagiwara, K Nagamachi, Y AF Takenoshita, S Mogi, A Osawa, H Sunaga, H Kakegawa, H Tani, M Morinaga, N Kato, R Hagiwara, K Nagamachi, Y TI Absence of mutatins in the analysis of coding sequences of the entire transforming growth factor beta type II receptor gene in sporadic human gastric cancer using genomic DNA and intron primers SO ONCOLOGY REPORTS LA English DT Article DE transforming growth factor beta type II receptor; gastric carcinogenesis; tumor biology ID TGF-BETA; MICROSATELLITE INSTABILITY; COLORECTAL-CANCER; CARCINOMA-CELLS; COLON; PROTEIN; BREAST; 18Q21; DPC4 AB Mutations in the transforming growth factor beta type II receptor (TGF beta RTII) gene have been detected in several human cancers that represent the phenotype of genomic instability. To establish a basis for diagnosis of cancer patients, we previously determined the exon-intron organization of the TGF beta RII gene. The results indicated that TGF beta RII protein is encoded by 567 codons in 7 exons. In this study, we further determined the nucleotide sequences surrounding these 7 exons and designed 8 sets of intron-based primers to examine the entire coding region of the TGF beta RII gene. By using these primers, we screened for mutations of the TGF beta RII gene in DNAs of 32 sporadic gastric cancer patients in whom one case showed MI+ (3.1%) at two loci. We found no mutations, and these data support other recent evidence that TGF beta RII mutations rarely occur except in colon and gastric tumors with MI. C1 Gunma Univ, Sch Med, Dept Surg 1, Gunma 371, Japan. Natl Canc Ctr, Res Inst, Div Biol, Tokyo 104, Japan. NCI, Human Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. RP Takenoshita, S (reprint author), Gunma Univ, Sch Med, Dept Surg 1, 3-39-22 Showamachi, Gunma 371, Japan. NR 24 TC 2 Z9 2 U1 0 U2 0 PU PROFESSOR D A SPANDIDOS PI ATHENS PA 1, S MERKOURI ST, EDITORIAL OFFICE,, ATHENS 116 35, GREECE SN 1021-335X J9 ONCOL REP JI Oncol. Rep. PD JAN-FEB PY 1998 VL 5 IS 1 BP 77 EP 80 PG 4 WC Oncology SC Oncology GA YN734 UT WOS:000071200700015 PM 9458298 ER PT J AU Li, QD Bever, CT AF Li, QD Bever, CT TI Effect of protein kinase modulators on the regulation of cathepsin B activity in THP-1 human monocytic leukemia cells SO ONCOLOGY REPORTS LA English DT Article DE lipopolysaccharide; IFN-gamma; cathepsin B; THP-1 cells; protein kinase C; cAMP-dependent protein kinase ID MURINE PERITONEAL-MACROPHAGES; NITRIC-OXIDE SYNTHESIS; BACTERIAL LIPOPOLYSACCHARIDE; TNF-ALPHA; IFN-GAMMA; SELECTIVE INHIBITOR; MOUSE MACROPHAGE; HUMAN PROSTATE; EXPRESSION; ACTIVATION AB Cathepsin B (CB), a lysosomal cysteine proteinase, is implicated in cancer metastasis and inflammatory tissue injury. We examined the effects of the protein kinase agonists and inhibitors on the regulation of CB activity in THP-1 human monocytic cells by two macrophage activators, lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma). CB elevation induced by LPS alone or LPS followed by IFN-gamma was blocked by protein kinase C (PKC) inhibitors staurosporine, H-7, phloretin and bisindolylmaleimide, and by cyclic nucleotide-dependent protein kinase inhibitors HA 1004, H-8, H-89 and cAMP-dependent protein kinase (PKA) inhibitor. The CB activity by LPS and IFN-gamma were augmented by diacylglycerol kinase inhibitor. PKC activator, phorbol 12-myristate 13-acetate (PMA) and PKA activator, dibutyryl cAMP could replace LPS in priming the cells for IFN-gamma stimulation but 8-bromo-cGMP did not. These findings suggest that the activation of PKC and PKA appears to be involved at least in part in the induction of CB activity in THP-1 cells. C1 Baltimore Vet Affairs Med Ctr, Med Res Serv, Baltimore, MD 21201 USA. Univ Maryland, Sch Dent, Dept Microbiol, Baltimore, MD 21201 USA. Univ Maryland, Sch Med, Dept Neurol, Baltimore, MD 21201 USA. Univ Maryland, Sch Med, Dept Pharmacol & Expt Therapeut, Baltimore, MD 21201 USA. RP Li, QD (reprint author), NCI, Med Branch, Med Ovarian Canc Sect, NIH, Bldg 10,Room 13N248, Bethesda, MD 20892 USA. NR 64 TC 7 Z9 7 U1 0 U2 0 PU PROFESSOR D A SPANDIDOS PI ATHENS PA 1, S MERKOURI ST, EDITORIAL OFFICE,, ATHENS 116 35, GREECE SN 1021-335X J9 ONCOL REP JI Oncol. Rep. PD JAN-FEB PY 1998 VL 5 IS 1 BP 227 EP 233 PG 7 WC Oncology SC Oncology GA YN734 UT WOS:000071200700045 PM 9458327 ER PT J AU Thimmaiah, KN Jayashree, BS Germain, GS Houghton, PJ Horton, JK AF Thimmaiah, KN Jayashree, BS Germain, GS Houghton, PJ Horton, JK TI Characterization of 2-chloro-N-10-substituted phenoxazines for reversing multidrug resistance in cancer cells SO ONCOLOGY RESEARCH LA English DT Article DE multidrug resistance; P-glycoprotein; resistance modulators ID PROPAFENONE-TYPE MODULATORS; VINCA ALKALOID RESISTANCE; HUMAN-LEUKEMIC CELLS; HAMSTER OVARY CELLS; P-GLYCOPROTEIN; CYTO-TOXICITY; PHASE-I; VINBLASTINE; VERAPAMIL; MEMBRANE AB Twenty-one 2-chloro-N-10-substituted phenoxazines have been synthesized and characterized as potential modulators of multidrug resistance (MDR). Many of the compounds, at a concentration of 100 mu M, enhanced accumulation of vinblastine (VLB) in drug-resistant KB8-5 cells to a greater extent than the same concentration of verapamil (VRP). However, the effects on VLB accumulation were specific, because these derivatives had little activity in the parental drug-sensitive line KB3-1. The compounds slowed the efflux of VLB from KB8-5 cells, suggesting that the chlorophenoxazines, like VRP, can inhibit P-glycoprotein (P-gp)-mediated efflux of VLB from this cell line. Two of the chlorophenoxazine derivatives, and also VRP, were able to stimulate the vanadate-sensitive ATPase activity attributable to P-gp in membranes isolated from MDR1 baculovirus-infected Sf9 cells. This result suggests that these modulators exert their effect by directly interacting with P-gp. Apart from the parent unsubstituted molecule, 2-chlorophenoxazine, there was a good correlation between log(10)P and the ability of the compounds to enhance VLB accumulation in KB8-5. This suggests that lipophilicity of a modulator is important, but is not the sole determinant of potency. Within this series of compounds, the optimal structural features for MDR modulation include a hydrophobic phenoxazine ring with a -Cl atom in the C-2 position and a tertiary amine group four carbons from the tricyclic ring. Many of the agents at the IC10 concentration completely reversed the 37-fold VLB resistance in KB8-5 cells. The most active agents in KB8-5 were able to partially reverse VLB resistance in an MDR colon carcinoma cell line GC(3)/c1 and completely reversed the 86-fold VLB resistance in the MDR1-overexpressing breast carcinoma cell line BC19/3. These same agents could only partially sensitize BC19/3 cells to taxol and doxorubicin, suggesting that the chlorophenoxazine derivatives show some specificity for modulating VLB resistance. C1 Univ Mysore, Dept Studies Chem, Mysore 570006, Karnataka, India. St Jude Childrens Res Hosp, Dept Mol Pharmacol, Memphis, TN 38105 USA. Univ Texas, Med Branch, Sealy Ctr Mol Sci, Galveston, TX 77555 USA. RP Horton, JK (reprint author), NIEHS, Struct Biol Lab, POB 12233,MD F3-01,111 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. OI iyengar, jayashree/0000-0003-1729-5805 FU NCI NIH HHS [CA-21765, CA-23099] NR 49 TC 25 Z9 25 U1 0 U2 1 PU COGNIZANT COMMUNICATION CORP PI ELMSFORD PA 3 HARTSDALE ROAD, ELMSFORD, NY 10523-3701 USA SN 0965-0407 J9 ONCOL RES JI Oncol. Res. PY 1998 VL 10 IS 1 BP 29 EP 41 PG 13 WC Oncology SC Oncology GA ZM518 UT WOS:000073548200005 PM 9613455 ER PT J AU Taimi, M Chen, ZX Breitman, TR AF Taimi, M Chen, ZX Breitman, TR TI Potentiation of retinoic acid-induced differentiation of human acute promyelocytic leukemia NB4 cells by butyric acid, tributyrin, and hexamethylene bisacetamide SO ONCOLOGY RESEARCH LA English DT Article DE drug synergism; cell differentiation; drug combinations; retinoic acid; butyrates ID BINDING-PROTEIN CRABP; TERATOCARCINOMA CELLS; SODIUM-BUTYRATE; LINE HL-60; IN-VITRO; THERAPY; EXPRESSION; METABOLISM; PHARMACOKINETICS; INDUCTION AB Cytodifferentiation therapy by all-trans-retinoic acid (RA) for acute promyelocytic leukemia patients is encouraging in spite of several limitations preventing better clinical outcomes, Most patients in complete remission induced by RA experience relapse and resist further treatment with RA. This resistance primarily is due to a systemic self-induced catabolism of RA, which interferes with the maintenance of effective plasma levels of RA. In this report we explored the possibility that treatment with combinations of RA and other differentiation agents may induce differentiation at lower RA concentrations, which in turn may produce diminished levels of resistance. We found that although n-butyric acid (BA), tributyrin (TB) (a prodrug of BA), or hexamethylene bisacetamide (HMBA) were inactive as sole agents they potentiated RA-induced differentiation of human acute promyelocytic NB4 cells. A measure of the effectiveness of these combinations was that the concentrations of RA in combination with BA and HMBA inducing half-maximal differentiation were 20- to 40-fold lower than those needed with RA alone. Furthermore, the concentrations of BA and HMBA in these combinations were at achievable plasma levels. Therefore, these combinations may have clinical utility for treatment of a variety of malignancies that are sensitive to RA alone. C1 NCI, Div Basic Sci, Chim Biol Lab, NIH, Bethesda, MD 20892 USA. Suzhou Med Coll, Affiliated Hosp 1, JiangSu Inst Hematol, Suzhou 215006, Peoples R China. RP Breitman, TR (reprint author), NCI, Div Basic Sci, Chim Biol Lab, NIH, Bldg 37,Room 5D-02, Bethesda, MD 20892 USA. NR 47 TC 15 Z9 16 U1 0 U2 0 PU COGNIZANT COMMUNICATION CORP PI ELMSFORD PA 3 HARTSDALE ROAD, ELMSFORD, NY 10523-3701 USA SN 0965-0407 J9 ONCOL RES JI Oncol. Res. PY 1998 VL 10 IS 2 BP 75 EP 84 PG 10 WC Oncology SC Oncology GA ZX683 UT WOS:000074543500004 PM 9666515 ER PT J AU Fontana, JA Sun, RJ Rishi, AK Dawson, MI Ordonez, JV Zhang, YX Tschang, SH Bhalla, K Han, ZY Wyche, J Poirier, G Sheikh, MS Shroot, B Reichert, U AF Fontana, JA Sun, RJ Rishi, AK Dawson, MI Ordonez, JV Zhang, YX Tschang, SH Bhalla, K Han, ZY Wyche, J Poirier, G Sheikh, MS Shroot, B Reichert, U TI Overexpression of bcl-2 or bcl-X-L fails to inhibit apoptosis mediated by a novel retinoid SO ONCOLOGY RESEARCH LA English DT Article DE retinoids; bcl-2; bcl-X-L; apoptosis ID LEUKEMIA HL-60 CELLS; CHEMOTHERAPY-INDUCED APOPTOSIS; HUMAN BREAST-CARCINOMA; KINASE-C-DELTA; SIGNAL-TRANSDUCTION; IN-VIVO; CHROMOSOMAL BREAKPOINT; PROTEASE ACTIVATION; GENE-EXPRESSION; CYTOCHROME-C AB Overexpression of bcl-2 or bcl-X-L has been found to inhibit the induction of apoptosis in malignant cells by a large number of agents including a wide variety of chemotherapeutic drugs. CD437 {6-[3-(1-adamantyl)-4 hydroxyphenyl]-2-naphthalene carboxylic acid} is a novel retinoid that induces apoptosis in a number of malignant cells through it unique mechanism of action. The addition of 1 mu M CD437 to HL-60/NEO cells resulted in capase 3 (CPP32) activation and poly(ADP-ribose) polymerase (PARP) cleavage in 3 h whereas in bcl-2- or bcl-X-L-overexpressing HL-60 cells CD437 induced CPP32 activation and PARP cleavage in 6 h. Although 50 and 300 nM CD437 were required to induce PARP cleavage in HL-60/NEO and HL-60/bcl-2, HL-60/bcI-X-L cells, respectively, maximal apoptosis in both cell lines was achieved utilizing 300 nM CD437. All three cell lines, however, share identical dose-response curves in terms of their growth inhibition, suggesting that CD437-mediated inhibition of growth and induction of apoptosis represent two distinct and separable processes. In addition, CD437 induces G(1) arrest as well as p21(WAFL/CIPI) mRNA expression in these cells despite the overexpression of bcl-2 or bcl-X-L. CD437 induced mitochondrial instability as indicated by cytochrome c leakage into the cytoplasm in all three cell lines. CD437 also induced growth inhibition and apoptosis of an apoptosis-resistant variant of the HL-60 cell line (HCW-2), which switched expression from bcl-2 to bcl-XL. CD437-mediated apoptosis is not accompanied by downregulation of bcl-2 or bci-X-L or upregulation of bar. The reason for the inability of bcl-2 or bcl-X-L overexpression to inhibit CD437-mediated apoptosis is unclear. The ability of CD437 to initiate apoptosis in a spectrum of malignant cells without interference from bcl-2 or bcl-X-L overexpression suggests that CD437 may possess significant therapeutic potential in the treatment of malignancy. C1 Univ Maryland, Marlene & Stewart Greenebaum Canc Ctr, Dept Med, Baltimore, MD 21201 USA. Vet Adm Med Ctr, Baltimore, MD 21201 USA. SRI Int, Retinoid Program, Menlo Pk, CA 94025 USA. Emory Univ, Sch Med, Winship Canc Ctr, Dept Med, Atlanta, GA 30322 USA. Brown Univ, Dept Mol Biol Cell Biol & Biochem, Providence, RI 02912 USA. CHU, Dept Mol Endocrinol, Poly ADP Ribose Metab Grp, St Foy, PQ, Canada. NIH, Mol Pharmacol Lab, Bethesda, MD 20892 USA. CIRD Galderma, F-06902 Valbonne, France. RP Fontana, JA (reprint author), John Dingle VA Med Ctr, 111 G,4646 John R St, Detroit, MI 48201 USA. OI Fontana, Joseph/0000-0003-3829-3358 FU NCI NIH HHS [P01 CA 51993, R01 CA 63335] NR 65 TC 17 Z9 18 U1 0 U2 0 PU COGNIZANT COMMUNICATION CORP PI ELMSFORD PA 3 HARTSDALE ROAD, ELMSFORD, NY 10523-3701 USA SN 0965-0407 J9 ONCOL RES JI Oncol. Res. PY 1998 VL 10 IS 6 BP 313 EP 324 PG 12 WC Oncology SC Oncology GA 139NM UT WOS:000077035600004 PM 9848102 ER PT J AU Castren, K Ranki, A Welsh, JA Vahakangas, KH AF Castren, K Ranki, A Welsh, JA Vahakangas, KH TI Infrequent p53 mutations in arsenic-related skin lesions SO ONCOLOGY RESEARCH LA English DT Article DE arsenic-related skin lesions; p53 mutations; squamous cell carcinoma; basal cell carcinoma ID CARCINOMA CELL-LINES; PROTEIN EXPRESSION; POINT MUTATIONS; BOWENS-DISEASE; GENE-MUTATIONS; CANCER; TUMORS; RAS; SENSITIVITY; DNA AB Oral arsenic exposure increases the risk for a variety of benign and malignant skin lesions, but the molecular mechanism of the carcinogenic effect is poorly understood. Arsenic-related squamous cell carcinomas of the skin can develop either de novo or progress from Bowen's disease lesions. Arsenic-related basal cell carcinomas develop usually in non-sun-exposed areas and are multiple. Because p53 tumor suppressor protein is a protective cellular molecule against environmental carcinogens and mutations in the p53 gene are frequent in nonmelanoma skin cancers, we studied p53 in 23 premalignant or malignant skin lesions from seven patients with a history of arsenic medication. The eighth patient studied (with six lesions) had a long-standing exposure to UV radiation. Accumulation of the p53 protein was detected (with a monoclonal DO-7 antibody) in 78% of the lesions from cases with arsenic exposure. Two of the six (30%) arsenic-related premalignant lesions and in addition one UV-related carcinoma in situ lesion were clearly and repeatedly positive when p53 exons 5 to 8 were screened by a nonradioactive single-strand conformation polymorphism (SSCP) analysis. Only one of the arsenic-related lesions was confirmed by sequencing to have a mutation (a CC to TT double transition). Na indications of mutations were found among the 18 basal cell carcinoma or two squamous cell carcinoma lesions studied. Our results suggest that the frequent accumulation of p53 protein in arsenic-related skin lesions is not due to p53 mutations, which may not be a prerequisite in the development of arsenic induced skin cancers. C1 Univ Oulu, Dept Pharmacol & Toxicol, FIN-90220 Oulu, Finland. Helsinki Univ Hosp, Dept Dermatol, Helsinki, Finland. Oulu Univ Hosp, Dept Dermatol, Oulu, Finland. NCI, Human Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. RP Vahakangas, KH (reprint author), Univ Oulu, Dept Pharmacol & Toxicol, FIN-90220 Oulu, Finland. EM kirsi.vahakangas@oulu.fi NR 30 TC 22 Z9 27 U1 0 U2 1 PU COGNIZANT COMMUNICATION CORP PI ELMSFORD PA 3 HARTSDALE ROAD, ELMSFORD, NY 10523-3701 USA SN 0965-0407 J9 ONCOL RES JI Oncol. Res. PY 1998 VL 10 IS 9 BP 475 EP 482 PG 8 WC Oncology SC Oncology GA 178YU UT WOS:000079297400006 PM 10223623 ER PT J AU Lesaca, EE Ensley, JF Yeudall, WA AF Lesaca, EE Ensley, JF Yeudall, WA TI Cellular factors may enable squamous carcinoma cells to overcome TGF beta-mediated repression of CDK2 activity SO ORAL ONCOLOGY LA English DT Article DE transforming growth factor; p21; WAF1; Cip1; cyclin dependent kinase; cell cycle; squamous cell carcinoma; oral cancer ID GROWTH-FACTOR-BETA; B-MYB; CYCLE ARREST; C-MYB; SIGNAL-TRANSDUCTION; POTENTIAL MEDIATOR; EPITHELIAL-CELLS; DEPENDENT KINASE; G(1) ARREST; HUMAN HEAD AB Cell lines developed from head and neck squamous cell carcinomas exhibit variable responses to the negative regulatory effects of transforming growth factor beta (TGF beta) on cell growth. To analyse the effects of TGF beta on regulators of cell cycle progression, we characterised cell lines derived from head and neck squamous cell carcinoma (HNSCC) for their biological sensitivities to TGF beta, growth inhibition, then examined the effects of TGF beta treatment on the expression and activity of cyclin dependent kinases (CDKs) and inhibitors of these kinases. Western blot analysis of cell lysates from untreated or TGF beta-treated cultures showed no alterations in expression of CDK2, CDK4, CDK6 or cyclin E in cell lines which were either sensitive (HaCaT, HN6) or refractory (HN12, HN30) to the growth-inhibitory effects of TGF beta. However, treatment of cells with TGF beta resulted in a several fold increase in cellular levels of p21 (WAF1/Cip1), irrespective of biological response. Immune complex in vitro kinase assays demonstrated that the activity of CDK2 was inhibited by exposure to ligand in each case, confirming that a TGF beta signalling pathway which regulates kinase activity was intact in these cell lines. The data suggest that cellular factors expressed in HN12 and HN30 enable these cells to override TGF beta-mediated inhibition of CDK2 activity and allow cell cycle progression. This may represent an important mechanism which allows cells to evade growth arrest during malignant progression. (C) 1998 Elsevier Science Ltd. All rights reserved. C1 NIDR, Mol Carcinogenesis Lab, Cellular Dev & Oncol Lab, Bethesda, MD 20892 USA. Wayne State Univ, Div Hematol Oncol, Detroit, MI USA. RP Yeudall, WA (reprint author), NIDR, Mol Carcinogenesis Lab, Cellular Dev & Oncol Lab, Bethesda, MD 20892 USA. NR 49 TC 2 Z9 2 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0964-1955 J9 ORAL ONCOL JI Oral Oncol. PD JAN PY 1998 VL 34 IS 1 BP 52 EP 57 DI 10.1016/S1368-8375(97)00023-7 PG 6 WC Oncology; Dentistry, Oral Surgery & Medicine SC Oncology; Dentistry, Oral Surgery & Medicine GA ZM348 UT WOS:000073529500009 PM 9659520 ER PT S AU Martin, A AF Martin, A BE Jablonski, NG Aiello, LC TI Organization of semantic knowledge and the origin of words in the brain SO ORIGIN AND DIVERSIFICATION OF LANGUAGE SE MEMOIRS OF THE CALIFORNIA ACADEMY OF SCIENCES LA English DT Proceedings Paper CT 3rd Wattis Symposium in Anthropology on the Origin and Diversification of Language CY APR 12, 1997 CL CALIF ACAD SCI, SAN FRANCISCO, CA SP Calif Acad Sci HO CALIF ACAD SCI ID HUMAN AMYGDALA; FACIAL EXPRESSIONS; RECOGNITION; COLOR; IMPAIRMENT; THINGS; DISSOCIATION; CATEGORIES; IMAGERY; SYSTEMS AB What does it mean to claim that a nonhuman species has words? Recent evidence from cognitive neuroscience indicates that meaning or semantic information about a particular object is represented as a distributed network of discrete cortical regions. Within this network the features that define an object are stored close to the sensory and motor regions of the brain that were active when information about that object was acquired. These semantic representations are active,whenever the object is perceived and when its name is produced or heard. The organization of semantic information parallels the organization of the sensory and motor systems in the primate brain. Evidence of similarities in the way object information is stored in the cerebral cortex: of human and nonhuman primates may provide a means for assessing the referential status of nonhuman vocalizations. C1 NIMH, Lab Brain & Cognit, Bethesda, MD 20892 USA. NR 54 TC 31 Z9 31 U1 1 U2 1 PU CALIFORNIA ACAD SCIENCES PI SAN FRANCISCO PA GOLDEN GATE PARK, SAN FRANCISCO, CA 94118 USA SN 0885-4629 BN 0-940228-44-0 J9 MEM CALIF ACAD SCI PY 1998 IS 24 BP 69 EP 88 PG 20 WC Anthropology; Language & Linguistics SC Anthropology; Linguistics GA BQ36C UT WOS:000088122000004 ER PT J AU Hochberg, MC Williamson, J Skinner, EA Guralnik, J Kasper, JD Fried, LP AF Hochberg, MC Williamson, J Skinner, EA Guralnik, J Kasper, JD Fried, LP TI The prevalence and impact of self-reported hip fracture in elderly community-dwelling women: The women's health and aging study SO OSTEOPOROSIS INTERNATIONAL LA English DT Article DE disability; fractures; osteoporosis ID RISK-FACTORS; MEDICAL CONDITIONS; OSTEOPOROSIS; DISABILITY; MORTALITY; SURVIVAL; OLDER; RATES; FALLS AB To estimate the prevalence and impact of self-reported hip fracture in elderly women an age-stratified random sample of 3841 community-dwelling women aged 65 years and above were interviewed to determine the occurrence of 13 chronic conditions and difficulty performing 15 tasks. Associations were examined using multiple logistic regression analysis. The weighted prevalence of hip fracture was 4.7 per 100. Prevalence increased with increasing age from 2.9 per 100 in women aged 65-74 years to 12.6 per 100 in women aged 85 years and above, and was higher in white women than black women. Women with hip fracture were significantly more likely to report concomitant Parkinson's disease (age-adjusted odds ratio [aOR] = 2.8) and stroke (aOR = 1.8). After adjustment for potential confounding variables, women with hip fracture were significantly more likely to report difficulty performing 11 activities that map into domains of mobility/exercise tolerance, self-care tasks and higher functioning domains. Hip fracture is common among elderly community-dwelling women and is associated with difficulty in performing activities of daily living. C1 Univ Maryland, Sch Med, Dept Med, Div Clin Immunol & Rheumatol, Baltimore, MD 21201 USA. Univ Maryland, Sch Med, Dept Epidemiol & Prevent Med, Baltimore, MD 21201 USA. Vet Affairs Maryland Hlth Care Syst, Ctr Geriatr Res Educ & Clin, Baltimore, MD USA. Johns Hopkins Univ, Sch Med, Dept Med, Div Gen Internal Med, Baltimore, MD 21205 USA. Johns Hopkins Univ, Sch Med, Dept Med, Welch Ctr Epidemiol Prevent & Clin Res, Baltimore, MD 21205 USA. Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Hlth Policy & Management, Baltimore, MD USA. NIA, Epidemiol Demog & Biometry Program, Bethesda, MD 20892 USA. RP Hochberg, MC (reprint author), 10 S Pine St,MSTF 8-34, Baltimore, MD 21201 USA. FU NIA NIH HHS [N01-AG12112] NR 33 TC 19 Z9 20 U1 0 U2 0 PU SPRINGER-VERLAG LONDON LTD PI GODALMING PA SWEETAPPLE HOUSE CATTESHALL ROAD, GODALMING GU7 3DJ, SURREY, ENGLAND SN 0937-941X J9 OSTEOPOROSIS INT JI Osteoporosis Int. PY 1998 VL 8 IS 4 BP 385 EP 389 DI 10.1007/s001980050079 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 186AE UT WOS:000079703500012 PM 10024910 ER PT J AU Looker, AC Wahner, HW Dunn, WL Calvo, MS Harris, TB Heyse, SP Johnston, CC Lindsay, R AF Looker, AC Wahner, HW Dunn, WL Calvo, MS Harris, TB Heyse, SP Johnston, CC Lindsay, R TI Updated data on proximal femur bone mineral levels of US adults SO OSTEOPOROSIS INTERNATIONAL LA English DT Article DE bone mineral density; proximal femur ID HIP FRACTURE; NATIONAL-HEALTH; NHANES-III; DENSITY; OLDER; RISK; OSTEOPOROSIS; POPULATION; PREVALENCE; SYMMETRY AB This paper describes data on bone mineral levels in the proximal femur of US adults based on the nationally representative sample examined during both phases of the third National Health and Nutrition Examination Survey (NHANES III 1988-94), and updates data previously presented from phase I only. The data were collected from 14646 men and women aged 20 years and older using dual-energy X-ray absorptiometry, and included bone mineral density (BMD), bone mineral content (BMC) and area of bone scanned in four selected regions of interest (ROI) in the proximal femur: femur neck, trochanter, intertrochanter and total. These variables are provided separately by age and sex for non-Hispanic whites (NHW), non-Hispanic blacks (NHB) and Mexican Americans (MA). NHW in the southern United States had slightly lower BMD levels than NHW in other US regions, but these differences were not sufficiently large to prevent pooling of the data. The updated data provide valuable reference data on femur bone mineral levels of noninstitutionalized adults. The updated data on BMD for the total femur ROI of NHW have been selected as the reference database for femur standardization efforts by the International Committee on Standards in Bone Measurements. C1 Ctr Dis Control & Prevent, Natl Ctr Hlth Stat, Div Hlth Examinat Stat, Hyattsville, MD 20782 USA. Mayo Clin & Mayo Fdn, Rochester, MN 55905 USA. US FDA, Ctr Food Safety & Appl Nutr, Washington, DC 20204 USA. NIA, NIH, Bethesda, MD 20892 USA. NIAMSD, NIH, Bethesda, MD 20892 USA. Indiana Univ, Med Ctr, Indianapolis, IN USA. Helen Hayes Hosp, Reg Bone Ctr, W Haverstraw, NY USA. RP Looker, AC (reprint author), Ctr Dis Control & Prevent, Natl Ctr Hlth Stat, Div Hlth Examinat Stat, Room 900,6525 Belcrest Rd, Hyattsville, MD 20782 USA. NR 28 TC 660 Z9 673 U1 3 U2 5 PU SPRINGER-VERLAG LONDON LTD PI GODALMING PA SWEETAPPLE HOUSE CATTESHALL ROAD, GODALMING GU7 3DJ, SURREY, ENGLAND SN 0937-941X J9 OSTEOPOROSIS INT JI Osteoporosis Int. PY 1998 VL 8 IS 5 BP 468 EP 489 DI 10.1007/s001980050093 PG 22 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 186AF UT WOS:000079703600013 PM 9850356 ER PT B AU Takahashi, S Sokoloff, L AF Takahashi, S Sokoloff, L BE Ishimura, Y Shimada, H Suematsu, M TI Role of nitric oxide in the regulation of cerebral blood flow in conscious rats SO OXYGEN HOMEOSTASIS AND ITS DYNAMICS SE KEIO UNIVERSITY SYMPOSIA FOR LIFE SCIENCE AND MEDICINE LA English DT Proceedings Paper CT Conference on Oxygen Homeostasis and Its Dynamics CY DEC 08-13, 1996 CL UNIV KEIO, TOKYO, JAPAN SP Kieo Univ Med Sci Fund HO UNIV KEIO DE autoradiographic [C-14]iodoantipyrine method; N-G-nitro-L-arginine methyl ester; whisker barrel; autoregulation; carbon dioxide ID GLUCOSE-UTILIZATION; RELAXING FACTOR; INHIBITION; SYNTHASE; BRAIN AB Effects of inhibition of brain nitric oxide (NO) synthase on the cerebral vasodilation evoked by(1) functional activation, (2) autoregulatory response to hypotension, and (3) 5% CO2 inhalation were studied in conscious rats with the [C-14]iodoantipyrine method for measurement of local cerebral blood flow (CBF). Enzymatic assays confirmed that three regimens of N-G-nitro-L-arginine methyl ester (L-NAME) administration, that is, (1) single intravenous injection (30 mg/kg), (2) intracisternal infusion (approximately 10 fold the total estimated content of L-arginine in rat brain), and (3) chronic intraperitoneal administration (50 mg/kg twice daily for 4 days) reduced NO synthase activity in whole brain to 47%, 12%, and 16% of control levels, respectively. Percent increases in local CBF in the stations of the whisker-to-cortical barrel sensory pathway elicited by unilateral vibrissal stimulation was unchanged by any of the three regimens of NO synthase inhibition. After almost complete inhibition of NO synthase activity by chronic intraperitoneal injection of L-NAME, neither global nor local CBF was affected when arterial blood presure was lowered by controlled withdrawal of blood, indicating that the normal autoregulatory vasodilator response was preserved. Finally, the percent enhancement of average blood flow in the brain as a whole by inhalation of 5% CO2 in the inspired air was also unaltered following NO synthase inhibition by chronic intraperitoneal injection of L-NAME. In most of these experimental paradigms the NO synthase inhibition resulted in reduced baseline CBF despite increased systemic blood pressure. These results indicate that NO plays a role in the tonic regulation of cerebral vascular tone, but in conscious rats it does not mediate the increases in CBF produced by functional activation or increased blood CO2 tension or the autoregulatory cerebral vasodilatation in response to hypotension. C1 NIMH, Cerebral Metab Lab, Bethesda, MD 20892 USA. RP Takahashi, S (reprint author), NIMH, Cerebral Metab Lab, Bldg 36,Room 1A-05, Bethesda, MD 20892 USA. NR 35 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER-VERLAG TOKYO PI TOKYO PA 37-3, HONGO 3-CHOME BONKYO-KU, TOKYO, 113, JAPAN BN 4-431-70202-4 J9 KEIO UNIV SYMP LIFE PY 1998 VL 1 BP 518 EP 536 PG 19 WC Biochemistry & Molecular Biology; Physiology SC Biochemistry & Molecular Biology; Physiology GA BN54W UT WOS:000082188300066 ER PT J AU Shahabuddin, M AF Shahabuddin, M TI Plasmodium ookinete development in the mosquito midgut: a case of reciprocal manipulation SO PARASITOLOGY LA English DT Article DE insect; malaria; mosquito; Plasmodium; vector ID ANOPHELES-STEPHENSI LISTON; AEDES-AEGYPTI L; TRANSMISSION-BLOCKING ANTIBODIES; CULEX-TARSALIS COQUILLETT; PERITROPHIC MEMBRANE; MALARIA PARASITES; SURFACE PROTEIN; DIROFILARIA-IMMITIS; SEXUAL DEVELOPMENT; REFRACTORY STRAIN AB The ookinete is one of the most important stages of Plasmodium development in the mosquito. It is morphologically and biochemically distinct from the earlier sexual stages - gametocytes and zygote, and from the later stages - oocyst and sporozoites. Development to ookinete allows the parasite to escape from the tightly packed blood bolus, to cross the sturdy peritrophic matrix (PM), to be protected from the digestive environment of the midgut lumen, and to invade the gut epithelium. The success of each of these activities may depend on the degree of the biochemical and physical barriers in the mosquito (such as density of blood bolus, thickness of peritrophic matrix, proteolytic activities in the gut lumen etc.) and the ability of the ookinete to overcome these barriers. Ookinete motility, secretion of chitinase, resistance to the digestive enzymes, and recognition/invasion of the midgut epithelium all may play crucial roles in the transformation to oocyst. The overall sporogonic development of Plasmodium, therefore, depends on the results of the two-way manipulations between the parasite and the vector mosquito. Study of ookinete development and of the cellular and biochemical complexities of the mosquito gut may therefore lead to the design of novel strategies to block the transmission of malaria. This article reviews the intricate interactions between the parasite and the mosquito midgut in the context of development and transmission of Plasmodium parasites. C1 NIAID, Med Entomol Sect, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. RP Shahabuddin, M (reprint author), NIAID, Med Entomol Sect, Parasit Dis Lab, NIH, Bldg 4,Room B2-37, Bethesda, MD 20892 USA. NR 94 TC 16 Z9 16 U1 1 U2 5 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 USA SN 0031-1820 J9 PARASITOLOGY JI Parasitology PY 1998 VL 116 SU S BP S83 EP S93 PG 11 WC Parasitology SC Parasitology GA 100CU UT WOS:000074797400010 PM 9695113 ER PT J AU Kaler, SG AF Kaler, SG TI Metabolic and molecular bases of Menkes disease and occipital horn syndrome SO PEDIATRIC AND DEVELOPMENTAL PATHOLOGY LA English DT Review DE Menkes disease; occipital horn syndrome; copper transport; X chromosome; messenger RNA; splice junction mutation ID KINKY-HAIR DISEASE; LYSYL OXIDASE ACTIVITY; PERINATAL COPPER DEFICIENCY; PEPTIDE ALPHA-AMIDATION; EHLERS-DANLOS SYNDROME; X-LINKED DISEASE; PRENATAL-DIAGNOSIS; CANDIDATE GENE; TRANSPORTING ATPASE; HISTIDINE TREATMENT AB Menkes disease and occipital horn syndrome (OHS) are related disorders of copper transport that involve abnormal neurodevelopment, connective tissue problems, and often premature death. Location of the gene responsible for these conditions on the X chromosome was indicated by pedigree analysis from the time of these syndromes' earliest descriptions. Characterization of an affected female with an X-autosomal translocation was used to identify the Menkes/OHS gene, which encodes a highly evolutionarily conserved, copper-transporting P-type ATPase. The gene normally is expressed in nearly all human tissues, and it localizes to the trans-Golgi net work of cells. However, in over 70% of Menkes and OHS patients studied, expression of this gene has been demonstrated to be abnormal. Major gene deletions detectable by Southern blotting account for 15-20% of patients, and an interesting spectrum of other mutations is evident among 58 families whose precise molecular defects have been reported as of this writing. The center region of the gene seems particularly prone to mutation, and those that influence mRNA processing and splicing appear to be relatively common. Further advances in understanding the molecular and cell biological mechanisms involved in normal copper transport may ultimately yield new and better approaches to the management of these disorders. C1 NINDS, Clin Neurosci Branch, NIH, Bethesda, MD 20892 USA. Childrens Natl Med Ctr, Dept Lab Med, Washington, DC 20010 USA. RP Kaler, SG (reprint author), NINDS, Clin Neurosci Branch, NIH, Bethesda, MD 20892 USA. NR 169 TC 69 Z9 70 U1 0 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 1093-5266 J9 PEDIATR DEVEL PATHOL JI Pediatr. Dev. Pathol. PD JAN-FEB PY 1998 VL 1 IS 1 BP 85 EP 98 PG 14 WC Pathology; Pediatrics SC Pathology; Pediatrics GA 142JG UT WOS:000077196700011 PM 10463276 ER PT J AU Heyman, RB Joffe, A Anglin, TM Fuller, PG McDonald, CA Rogers, PD Shah, RZ Armentano, M Boyd, GM Czechowicz, D AF Heyman, RB Joffe, A Anglin, TM Fuller, PG McDonald, CA Rogers, PD Shah, RZ Armentano, M Boyd, GM Czechowicz, D TI Tobacco, alcohol, and other drugs: The role of the pediatrician in prevention and management of substance abuse SO PEDIATRICS LA English DT Article ID ADOLESCENTS; YOUTH AB During the past three decades, the responsibility of pediatricians to their patients and their patients' families regarding the prevention of substance abuse and the diagnosis and management of problems related to substance abuse has increased. The American Academy of Pediatrics (AAP) has highlighted the importance of such issues in a variety of ways, including its guidelines for preventive services. Nonetheless, many pediatricians remain reluctant to address this issue. The harmful consequences of tobacco, alcohol, and other drug use are a concern of medical professionals who care for infants, children, adolescents, and young adults. Thus, pediatricians should include discussion of substance abuse as a part of routine health care, starting with the prenatal visit and as a part of ongoing anticipatory guidance. Knowledge of the extent and nature of the consequences of tobacco, alcohol, and other drug use as well as the physical, psychological, and social consequences is important for pediatricians. Pediatricians should incorporate substance abuse prevention into daily practice, acquire the skills necessary to identify young people at risk for substance abuse, and provide or obtain assessment, intervention, and treatment as necessary. C1 NIAAA, Rockville, MD USA. NIDA, Lexington, KY 40583 USA. NR 28 TC 44 Z9 45 U1 1 U2 1 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD JAN PY 1998 VL 101 IS 1 BP 125 EP 128 PG 4 WC Pediatrics SC Pediatrics GA YP941 UT WOS:000071331400036 ER PT J AU Halsey, NA Abramson, JS Chesney, PJ Fisher, MC Gerber, MA Gromisch, DS Kohl, S Marcy, SM Murray, DL Overturf, GD Whitley, RJ Yogev, R Peter, G Hall, CB Schwartz, B Breiman, R Hardegree, MC Jacobs, RF MacDonald, NE Orenstein, WA Rabinovich, NR AF Halsey, NA Abramson, JS Chesney, PJ Fisher, MC Gerber, MA Gromisch, DS Kohl, S Marcy, SM Murray, DL Overturf, GD Whitley, RJ Yogev, R Peter, G Hall, CB Schwartz, B Breiman, R Hardegree, MC Jacobs, RF MacDonald, NE Orenstein, WA Rabinovich, NR TI Age for routine administration of the second dose of measles-mumps-rubella vaccine SO PEDIATRICS LA English DT Article ID SCHOOL POPULATION; ANTIBODY; REVACCINATION; OUTBREAK; FAILURE; PERSISTENCE; IMMUNITY; SERUM AB The purpose of this statement is to inform physicians of a modification in the recommendation of the appropriate age for routine administration of the second dose of measles-mumps-rubella (MMR) vaccine. The implementation of the two-dose measles vaccine schedule has improved the control of measles, but some outbreaks continue to occur in school children, although greater than or equal to 95% of children in school have received cine dose of vaccine. Because most measles vaccine failures are attributable to failure to respond to the first dose, that all children receive two doses of measles-containing vaccine is essential for the control of measles. Routine administration of the second dose of MMR vaccine at school entry (4 to 6 years of age) will help prevent school-based outbreaks. Physicians should continue to review the records of all children 11 to 12 years of age to be certain that they have received two doses of MMR vaccine after their first birthday. Documenting that all school children have received two doses of measles-containing vaccine by the year 2001 will help ensure the elimination of measles in the United States and contribute to the global effort to control and possibly eradicate measles. C1 Ctr Dis Control & Prevent, Atlanta, GA 30333 USA. US FDA, Rockville, MD 20857 USA. Amer Thorac Soc, New York, NY USA. Canadian Paediat Soc, Ottawa, ON, Canada. NIAID, Bethesda, MD USA. NR 35 TC 21 Z9 21 U1 0 U2 0 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD JAN PY 1998 VL 101 IS 1 BP 129 EP 133 PG 5 WC Pediatrics SC Pediatrics GA YP941 UT WOS:000071331400037 ER PT J AU Halsey, NA Abramson, JS Chesney, PJ Fisher, MC Gerber, MA Gromisch, DS Kohl, S Marcy, SM Murray, DL Overturf, GD Whitley, RJ Yogev, R Peter, G Hall, CB Schwartz, B Breiman, R Hardegree, MC Jacobs, RF MacDonald, NE Orenstein, WA Rabinovich, NR AF Halsey, NA Abramson, JS Chesney, PJ Fisher, MC Gerber, MA Gromisch, DS Kohl, S Marcy, SM Murray, DL Overturf, GD Whitley, RJ Yogev, R Peter, G Hall, CB Schwartz, B Breiman, R Hardegree, MC Jacobs, RF MacDonald, NE Orenstein, WA Rabinovich, NR TI Severe invasive group A streptococcal infections: A subject review SO PEDIATRICS LA English DT Article ID TOXIC-SHOCK SYNDROME; GROUP-A STREPTOCOCCI; NECROTIZING FASCIITIS; PHARYNGEAL CARRIAGE; VARICELLA; CHILDREN; DISEASE; THERAPY; PATHOGENESIS; CLINDAMYCIN AB The course of severe invasive group A beta-hemolytic streptococcal (GABHS) infections is often precipitous, requiring prompt diagnosis and rapid initiation of appropriate therapy. Therefore, physicians must have a high index of suspicion of this disease, particularly in patients at increased risk (eg, those with varicella or diabetes mellitus). Although a relationship between the use of nonsteroidal antiinflammatory drugs and severe invasive GABHS infections has been suggested, at present data on which to base a clinical derision about the use or restriction of nonsteroidal antiinflammatory drugs in children with varicella are insufficient. When necrotizing fasciitis is suspected, prompt surgical drainage, debridement, fasciotomy, or amputation often is necessary. Many experts recommend intravenously administered penicillin G and clindamycin for the treatment of invasive GABHS infections on the basis of animal studies. Some evidence exists that intravenous immunoglobulin given in addition to appropriate antimicrobial and surgical therapy may be beneficial. Although chemoprophylaxis for household contacts of persons with invasive GABHS infections has been considered by some experts, the limited available data indicate that the risk of secondary cases is low (2.9 per 1000) and data about the effectiveness of any drug are insufficient to make recommendations. Because of the low risk of secondary cases of invasive GABHS infections in schools or child care facilities, chemoprophylaxis is not indicated in these settings. Routine immunization of all healthy children against varicella is recommended and is an effective means to decrease the risk of invasive GABHS infections. C1 Ctr Dis Control & Prevent, Atlanta, GA 30333 USA. US FDA, Rockville, MD 20857 USA. Amer Thorac Soc, New York, NY USA. Canadian Paediat Soc, Ottawa, ON, Canada. NIAID, Bethesda, MD USA. NR 32 TC 54 Z9 58 U1 0 U2 3 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD JAN PY 1998 VL 101 IS 1 BP 136 EP 140 PG 5 WC Pediatrics SC Pediatrics GA YP941 UT WOS:000071331400039 ER PT J AU Halsey, NA Abramson, JS Chesney, PJ Fisher, MC Gerber, MA Marcy, SM Murray, DL Overturf, GD Prober, CG Weiner, LB Whitley, RJ Yogev, R Peter, G Pickering, LK Baker, CJ Hirsch, A Jacobs, RF MacDonald, NE Schwartz, B Livengood, JR Hardegree, MC Rabinovich, NR Breiman, RF AF Halsey, NA Abramson, JS Chesney, PJ Fisher, MC Gerber, MA Marcy, SM Murray, DL Overturf, GD Prober, CG Weiner, LB Whitley, RJ Yogev, R Peter, G Pickering, LK Baker, CJ Hirsch, A Jacobs, RF MacDonald, NE Schwartz, B Livengood, JR Hardegree, MC Rabinovich, NR Breiman, RF TI Recommended childhood immunization schedule - United States, January-December 1998 SO PEDIATRICS LA English DT Article ID B VACCINES; INTERCHANGEABILITY; INFANTS C1 AAP, Council Pediat Practice, Elk Grove Village, IL USA. Amer Thorac Soc, New York, NY USA. Canadian Paediat Soc, Ottawa, ON, Canada. Ctr Dis Control & Prevent, Atlanta, GA 30333 USA. US FDA, Rockville, MD 20857 USA. NIAID, Bethesda, MD USA. NR 9 TC 6 Z9 6 U1 0 U2 0 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD JAN PY 1998 VL 101 IS 1 BP 154 EP 157 PG 4 WC Pediatrics SC Pediatrics GA YP941 UT WOS:000071331400042 ER PT J AU Buku, A Maulik, G Hook, WA AF Buku, A Maulik, G Hook, WA TI Bioactivities and secondary structure of Mast Cell Degranulating (MCD) peptide analogs SO PEPTIDES LA English DT Article DE MCD peptide analogs; histamine; oxygen radicals; circular dichroism ID PROTEIN CONFORMATION; CIRCULAR-DICHROISM AB Three analogs of Mast Cell Degranulating (MCD) peptide with C-terminal and one analog with N- and C-terminal deletions were synthesized and assayed for histamine releasing activity in mast cells. Des(20-22)-MCD and des(21)-MCD markedly decreased this activity. In des(16-17,21)-MCD this activity was completely abolished. By contrast, when the C-and N-termini were truncated in des(1-2,20-22)-MCD, the full activity of MCD peptide was restored. The possibility that these analogs trigger or inhibit oxygen radicals from neutrophils was examined in a cell-free system with a chemiluminescence assay. None of the above analogs exhibited inflammatory or anti-inflammatory activity. Changes in biological activities were correlated with structural changes, as seen by circular dichroism (CD) spectroscopy. (C) 1998 Elsevier Science Inc. C1 CUNY Mt Sinai Sch Med, Dept Physiol & Biophys, New York, NY 10029 USA. Univ Connecticut, Sch Med, Dept Surg, Farmington, CT 06030 USA. Harvard Univ, Sch Med, Dept Radiat Oncol, Boston, MA 02215 USA. NIDR, Clin Immunol Sect, Bethesda, MD 20892 USA. RP Buku, A (reprint author), Mt Sinai Med Ctr, Box 1218,1 Gustave L Levy Pl, New York, NY 10029 USA. NR 19 TC 9 Z9 11 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1998 VL 19 IS 1 BP 1 EP 5 DI 10.1016/S0196-9781(97)00253-2 PG 5 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA YN496 UT WOS:000071174400001 PM 9437730 ER PT J AU Moody, TW Mayr, CA Gillespie, TJ Davis, TP AF Moody, TW Mayr, CA Gillespie, TJ Davis, TP TI Neurotensin is metabolized by endogenous proteases in prostate cancer cell lines SO PEPTIDES LA English DT Article DE neurotensin; prostate cancer; proteases; neutral endopeptidase; neuroendocrine tumors ID ANGIOTENSIN-CONVERTING ENZYME; RAT-BRAIN; LUNG-CANCER; SYNAPTIC-MEMBRANES; BETA-ENDORPHIN; PEPTIDE; SOMATOSTATIN; CARCINOMA; RECEPTORS; CALCIUM AB The formation and processing of neurotensin (NT) by three prostate cancer cell lines was investigated. Neurotensin (NT) immunoreactivity was detected in conditioned media and extracts of LNCaP cells. Using HPLC techniques, the immunoreactivity extracted from LNCaP cells coeluted with synthetic NT standard. Metalloendopeptidase 3.4.24.15 activity was detected in PC-3, DU-145 and LNCaP cells, whereas high levels of neutral endopeptidase 3.4.24.11 activity was detected only in LNCaP cells. NT was relatively stable when incubated with PC-3 or D-145 cells but was rapidly degraded by LNCaP cells to NT1-11 and NT1-10. Phosphoramidon inhibited the metabolism of NT by LNCaP cells. These data suggest that NT is present in and metabolized by LNCaP cellular enzymes. (C) 1998 Elsevier Science Inc. C1 Univ Arizona, Coll Med, Hlth Sci Ctr, Dept Pharmacol, Tucson, AZ 85724 USA. NCI, Biomarkers & Prevent Res Branch, Rockville, MD 20850 USA. RP Davis, TP (reprint author), Univ Arizona, Coll Med, Hlth Sci Ctr, Dept Pharmacol, Tucson, AZ 85724 USA. RI davis, thomas/F-3244-2015 OI davis, thomas/0000-0001-8465-4973 NR 34 TC 20 Z9 21 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1998 VL 19 IS 2 BP 253 EP 258 DI 10.1016/S0196-9781(97)00306-9 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA YV308 UT WOS:000071810200007 PM 9493857 ER PT J AU Xu, H Lu, YF Partilla, JS Pinto, J Calderon, SN Matecka, D Rice, KC Lai, J Porreca, F Ananthan, S Rothman, RB AF Xu, H Lu, YF Partilla, JS Pinto, J Calderon, SN Matecka, D Rice, KC Lai, J Porreca, F Ananthan, S Rothman, RB TI Opioid peptide receptor studies .8. One of the mouse brain delta(NCX) binding sites is similar to the cloned mouse opioid delta receptor: Further evidence for heterogeneity of delta opioid receptors SO PEPTIDES LA English DT Article DE delta receptors; receptor subtypes; [D-Ala(2), D-Leu(5)]enkephalin ID RAT-BRAIN; FUNCTIONAL EXPRESSION; OPIATE RECEPTOR; ANTISENSE OLIGODEOXYNUCLEOTIDE; CDNA CLONING; ANTINOCICEPTION; PURIFICATION; SUBTYPES; MEMBRANES; D-PEN5>ENKEPHALIN AB Quantitative ligand binding studies resolved two subtypes of the delta opioid receptor, termed delta(ncx1) and delta(ncx2), in mouse brain membranes depleted of mu receptors by pretreatment with the irreversible ligand, BIT. The purpose of the present study was to compare the binding parameters, ligand-selectivity profile and pharmacological properties of the cloned mouse delta receptor (MDOR) stably expressed in a cell line to the delta(ncx) binding sites of mouse brain. [H-3][D-Ala(2),D-Leu(5)]enkephalin labeled a single binding site in membranes prepared from MDOR cells under several different assay conditions including BIT-pretreatment. The MDOR had high affinity for delta agonists and antagonists. [H-3][D-Ala(2),D-Leu(5)]enkephalin labeled two binding sites in mouse brain membranes depleted of mu receptors by pretreatment with BIT: the delta(ncx1) site (high affinity for DPDPE and deltorphin) and the delta(ncx2) site (low affinity for DPDPE and deltorphin). Some agents were moderately selective for the delta(ncx2) site: [pCl]DPDPE (10.9-fold), JP41 (5.9-fold) and JP45 (3.8-fold). The K-i values of 12 opioids at the mouse MDOR were determined. These values were highly correlated with their values at the delta(ncx1) site but not the delta(ncx2) site. These data suggest that the delta(ncx2) site may be distinct from the cloned delta opioid receptor. (C) 1998 Elsevier Science Inc. C1 NIDA, Clin Psychopharmacol Sect, Div Intramural Res, NIH, Baltimore, MD 21224 USA. NIDDK, Med Chem Lab, NIH, Bethesda, MD 20892 USA. Univ Arizona, Coll Med, Dept Pharmacol, Tucson, AZ 85724 USA. So Res Inst, Dept Organ Chem, Birmingham, AL 35255 USA. RP Rothman, RB (reprint author), NIDA, Clin Psychopharmacol Sect, Div Intramural Res, NIH, POB 5180,5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM rrothman@lrp.nida.nih.gov NR 42 TC 6 Z9 6 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1998 VL 19 IS 2 BP 343 EP 350 DI 10.1016/S0196-9781(97)00294-5 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA YV308 UT WOS:000071810200017 PM 9493867 ER PT J AU Yokoi, H Arima, H Kondo, K Murase, T Iwasaki, Y Yang, HYT Oiso, Y AF Yokoi, H Arima, H Kondo, K Murase, T Iwasaki, Y Yang, HYT Oiso, Y TI Antiserum against neuropeptide FF augments vasopressin release in conscious rats SO PEPTIDES LA English DT Article DE neuropeptide FF; vasopressin; osmoregulation; antiserum ID CENTRAL-NERVOUS-SYSTEM; ARGININE-VASOPRESSIN; PEPTIDES; MORPHINE; FLFQPQRFAMIDE; RECEPTORS; BRAIN AB We previously reported that centrally administered neuropeptide FF (NPFF) inhibited arginine vasopressin (AVP) release, In this study, immunoneutralization of central NPFF was performed to evaluate the role of endogenous NPFF in the regulation of AVP release. Intracerebroventricular (ICV) injection of antiserum against NPFF (Anti-NPFF) significantly augmented the plasma AVP increase induced by hyperosmolality [intraperitoneal injection of hypertonic. saline (600 mOsm/kg, 2% BW)] at 60 min after ICV injection compared with normal rabbit serum CNRS) (NRS: 4.20 +/- 0.30 pg/ml, Anti-NPFF: 5.83 +/- 0.46 pg/ml, p < 0.01), Anti-NPFF did not cause significant change in plasma osmolality, plasma volume or arterial blood pressure, This evidence indicates that endogenous NPFF might be physiologically involved in osmoregulation of the plasma AVP level through its inhibitory action. (C) 1998 Elsevier Science Inc. C1 Nagoya Univ, Sch Med, Dept Internal Med 1, Showa Ku, Nagoya, Aichi 466, Japan. NIMH, Ctr Neurosci, Lab Biochem Genet, Washington, DC 20032 USA. RP Yokoi, H (reprint author), Nagoya Univ, Sch Med, Dept Internal Med 1, Showa Ku, 65 Tsurumai Cho, Nagoya, Aichi 466, Japan. RI Arima, Hiroshi/I-7383-2014 OI Arima, Hiroshi/0000-0003-3746-1997 NR 15 TC 9 Z9 9 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1998 VL 19 IS 2 BP 393 EP 395 DI 10.1016/S0196-9781(97)00375-6 PG 3 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA YV308 UT WOS:000071810200024 PM 9493874 ER PT J AU Moody, TW Leyton, J Unsworth, E John, C Lang, LX Eckelman, WC AF Moody, TW Leyton, J Unsworth, E John, C Lang, LX Eckelman, WC TI (Arg(15),Arg(21))VIP: Evaluation of biological activity and localization to breast cancer tumors SO PEPTIDES LA English DT Article DE VIP; receptors; cAMP; breast cancer; PACAP ID VASOACTIVE-INTESTINAL-PEPTIDE; FUNCTIONAL EXPRESSION; HIGH-AFFINITY; CELL-LINES; HUMAN-LUNG; RECEPTOR; POLYPEPTIDE; VIP; PACAP; ANTAGONIST AB VIP analogs, which contain a single lysine amino acid, were synthesized and evaluated using breast cancer cells. (Arg(15), Arg(20)) VIP, (Arg(15), Arg(21)) VIP, and (Arg(20), Arg(21)) VIP inhibited I-125-VIP binding to T47D cells with high affinity (IC50 values of 1.2, 1.0, and 0.8 nM, respectively). The VIP analogs elevated cAMP in T47D cells with ED50 values ranging from 0.1-1 nM. Because (Arg(15), Arg(21)) VIP was the most potent at elevating cAMP, it was characterized further. (Arg(15), Arg(21)) VIP transiently increased c-fos gene expression in breast cancer cells. N-Succinimidyl-4-F-18 (fluoromethly) benzoate was prepared in one chemical step from N-succinimidyl-4-(4-nitrobenzenesulfonyl)oxomethyl)benzoate by adding F-18 in acetone at room temperature. This prosthetic group was then reacted with (Arg(15), Arg(21)) VIP ((RR) VIP). (F-18-RR) VIP bound with high affinity to T47D cells and was rapidly internalized. (F-18-RR) VIP was injected intravenously into nude mice bearing breast cancer xenografts and after 4 h, the density of (F-18-RR) VIP was elevated in the tumors relative to normal organs. These data suggest that VIP receptors may be used to localize breast cancer tumors. (C) 1998 Elsevier Science Inc. C1 NCI, Cell & Canc Biol Dept, Med Branch, Rockville, MD 20850 USA. George Washington Univ, Med Ctr, Dept Radiopharmaceut Chem, Washington, DC 20037 USA. Ctr Clin, Positron Emiss Tomog Dept, Bethesda, MD 20892 USA. RP Moody, TW (reprint author), KWC, Med Branch, Rm 300,9610 Med Ctr Dr, Rockville, MD 20876 USA. EM moodyt@bprb.nci.nih.gov NR 30 TC 43 Z9 43 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1998 VL 19 IS 3 BP 585 EP 592 DI 10.1016/S0196-9781(97)00459-2 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA ZA658 UT WOS:000072388100021 PM 9533649 ER PT J AU Heyliger, SO Payza, K Rothman, RB AF Heyliger, SO Payza, K Rothman, RB TI The effect of FMRFamide analogs on [S-35]GTP-gamma-S stimulation in squid optic lobes SO PEPTIDES LA English DT Article DE agonist; FMRFamide; FLRFamide; desamino-Tyr-Phe-norLeu-Arg-Phe-amide; [S-35]GTP-gamma-S; partial agonist; superagonist ID OPIOID BINDING-SITES; HELIX-ASPERSA; SPINAL-CORD; G-PROTEINS; RAT-BRAIN; NEUROPEPTIDE; PEPTIDES; RECEPTORS; AGONIST; MORPHINE AB Pharmacological study of Phe-Met-Leu-Phe-amide (FMRFa) receptors is hindered by the lack of selective ligands. The classification of these selective ligands is further hampered by the limited availability of functional assays, In this study, we evaluated several synthetic FMRFa analogs for agonist and antagonist activity by measuring their abilities to produce [S-35]-GTP-gamma-S stimulation or to inhibit FMRFa-induced [S-35]-GTP-gamma-S binding in squid optic lobes, Analogs included acetyl-Phe-norLeu-Arg-Phe-amide (acFnLRFa), desamino-Tyr-Phe-Leu-Arg-amide (daYFLRa), desamino Tyr-Phe-norLeu-Arg-Phe-amide (daYFnLRFa), desamino Tyr-Phe-norLeu-Arg-[TIC]-amide (daYFnLR[TIC]a), desamino Tyr-Trp-norLeu-Arg-amide (daYWnLRa), (D)-Tyr-Phe-norLeu-Arg-Phe-amide (D-YFnLRFa), Phe-Leu-Arg-Phe-amide (FLRFa), and the D-amino acid analogs of FMRFa (D-FMRFa, F-(D)-MRFa and FM-(D)-RFa). For agonist studies, full dose-response curves were generated and analyzed for potency and efficacy (maximal percent effect). FMRF-amide as well as analogs ac-FnLRFa, daYFnLRFa, daYFnLR[TIC]a, D-YFnLRFa, FLRFa, and (D)-FMRFa stimulated [S-35]-GTP-gamma-S binding. Analogs daYWnLRa, daYFLRa, F-(D)-MRFa, and FM-(D)-RFa failed to stimulate either [S-35]-GTP-gamma-S binding or to inhibit FMRFa-induced [S-35]-GTP-gamma-S binding. The rank order of potency was daYFnLRFa greater than or equal to daYFnLRF[TIC]a > acFnLRFa > (D)YFnLRFa > FLRFa greater than or equal to FMRFa much greater than (D)-FMRFa. The order of efficacy was daYFnLRFa = acFnLRFa = (D)-YFnLRFa > FLRFa = FMRFa greater than or equal to (D)-FMRFa greater than or equal to daYFnLRF[TIC]a. Peptide analog daYFnLR[TIC]a was less efficacious (59%) maximal stimulation) than analogs daYFnLRFa, acFnLRFa, and (D)-YFnLRFa (113-146% maximal stimulation). A maximal concentration of daYFnLR[TIC]a (10 mu M) reduced daYFnLRFa, acFnLRFa, and (D)-YFnLRFa induced [S-35]-GTP-gamma-S stimulation, indicating that daYFnLR[TIC]a is a partial agonist at the receptor stimulated by the FMRFamide analogs. Analysis of the structural requirements needed fur promoting [S-35]-GTP-gamma-S binding show that elongation (i.e., daYFnLRFa, D-YFnLRFa) or modification of Phe(1) (ac-FnLRFa) leads to increased efficacy and potency. Moreover, elimination of the C-terminal Phe (daYWnLRa, daYFLRa,) leads to a loss of biological activity, However, substitution with L-1,2,3,4 tetrahydroisoquinoline-3-carboxylic acid, a rigid analog of the C-terminal Phe (daYFnLR[TIC]a), leads to decreased efficacy but not loss of potency. The data suggest that immobilization or modification of the C-terminal Phe may produce highly selective and potent FMRFamide antagonists. These results agree with published receptor radioligand studies and indicate that the [S-35]GTP-gamma-S assay may be useful in classifying novel FMRFamide-selective ligands. (C) 1998 Elsevier Science Inc. C1 NIDA, Clin Psychopharmacol Sect, Div Intramural Res, NIH, Baltimore, MD 21224 USA. ASTRA, Res Ctr Montreal, Montreal, PQ H4S 1Z9, Canada. RP Heyliger, SO (reprint author), NIDA, Clin Psychopharmacol Sect, Div Intramural Res, NIH, POB 5180,5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM SHEYLIG@IRP.NIDA.NIH.GOV; ROTHMAN@IRP.NIDA.NIH.GOV NR 54 TC 3 Z9 3 U1 1 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1998 VL 19 IS 4 BP 739 EP 747 DI 10.1016/S0196-9781(97)00481-6 PG 9 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA ZT341 UT WOS:000074075500016 PM 9622030 ER PT J AU Ni, Q Xu, H Partilla, JS Rice, KC Matecka, D Calderon, SN Porreca, F Lai, J Schmidhammer, H Krassnig, R Rothman, RB AF Ni, Q Xu, H Partilla, JS Rice, KC Matecka, D Calderon, SN Porreca, F Lai, J Schmidhammer, H Krassnig, R Rothman, RB TI Opioid peptide receptor studies. 9. Identification of a novel non-mu- non-delta-like opioid peptide binding site in rat brain SO PEPTIDES LA English DT Article DE opiate receptors; delta receptors; ligand binding; acetalin; antisense DNA ID GUINEA-PIG BRAIN; OPIATE RECEPTOR; BIOLOGICAL EVALUATION; ANTISENSE OLIGODEOXYNUCLEOTIDE; FUNCTIONAL EXPRESSION; KAPPA-RECEPTOR; MOUSE-BRAIN; SUBTYPES; CLONING; ANTINOCICEPTION AB Quantitative binding studies resolved two high-affinity [(3)H] [D-Ala(2), D-Leu(5)]enkephalin binding sites in rat brain membranes depleted of mu, binding sites by pretreatment with the irreversible agent BIT. -The two binding sites had lower (delta(ncx-2), Ki = 96.6 nM) and higher (delta(ncx-1), Ki = 1.55 nM) affinity for DPDPE. The ligand-selectivity profile of the delta(ncx-1), site was that of a classic delta binding site. The ligand-selectivity profile of the delta(ncx-2) site was neither mu- or delta-like. The Ki values of selected agents for the delta(ncx-2), site were: [pCl]DPDPE (3.9 nM), DPLPE (140 nhl), and DAMGO (2.6 nM). Under these assay conditions, [(3)H]CD-Ala(2),D-Leu(5)]enkephalin binding to the cells expressing the cloned CL receptor is very low and pretreatment of cell membranes with BIT almost completely inhibits [(3)H]DAMGO and [(3)H][D-Ala(2),D-Leu(5)]enkephalin binding. Intracerebroventricular administration of antisense DNA to the cloned delta receptor selectively decreased [(3)H][D-Ala2,D-Leu5]enkephalin binding to the delta(ncx-1) site. Administration of buprenorphine to rats 24 h prior to preparation of membranes differentially affected mu, delta(ncx-1), and delta(ncx-2), binding sites. Viewed collectively, these studies have identified a novel non-mu- non-delta-like binding site in rat brain. (C) 1998 Elsevier Science Inc. C1 NIDA, CPS, DIR, NIH, Baltimore, MD 21224 USA. NIDDKD, Med Chem Lab, NIH, Bethesda, MD 20892 USA. Univ Arizona, Coll Med, Dept Pharmacol, Tucson, AZ 85724 USA. Univ Innsbruck, Inst Pharmaceut Chem, A-6020 Innsbruck, Austria. RP Rothman, RB (reprint author), NIDA, CPS, DIR, NIH, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. NR 56 TC 6 Z9 7 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1998 VL 19 IS 6 BP 1079 EP 1090 DI 10.1016/S0196-9781(98)00046-1 PG 12 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA 104LA UT WOS:000075014900017 PM 9700759 ER PT J AU Haidan, A Hilbers, U Bornstein, SR Ehrhart-Bornstein, M AF Haidan, A Hilbers, U Bornstein, SR Ehrhart-Bornstein, M TI Human adrenocortical NCI-H295 cells express VIP receptors. Steroidogenic effect of vasoactive intestinal peptide (VIP) SO PEPTIDES LA English DT Article DE NCI-H295; steroidogenesis; VIP; VIP receptors ID GASTRIC-INHIBITORY POLYPEPTIDE; HUMAN ADRENAL-GLAND; ALDOSTERONE SECRETION; CUSHINGS-SYNDROME; IN-VITRO; MESSENGER-RNA; CANCER GROWTH; TUMORS; ANTAGONIST; LINE AB VIP receptors are frequently overexpressed by various endocrine tumors. In this study the expression of VIP receptors in the human adrenocortical carcinoma cell line NCI-H295 and their involvement in the regulation of steroidogenesis was investigated. NCI-H295 cells express VIP1 and VIP2 receptors as demonstrated by RT-PCR, whereas they do not express VIP itself. The receptors are functionally coupled to steroidogenesis since VIP (10(-9) M to 10(-6) M) exerted a dose-dependent stimulatory effect on the release of aldosterone, cortisol, and DHEA. VIP increased ACTH-stimulated releases of aldosterone and cortisol. The proliferation rate of NCI-H295 cells was not affected by VIP. These data show that NCI-H295 cells express both forms of the VIP receptor and that VIP is involved in an ACTH-independent regulation of steroidogenesis in the adrenal tumor cells. (C) 1998 Elsevier Science Inc. C1 Univ Leipzig, Med Klin & Poliklin 3, Dept Internal Med 3, D-04103 Leipzig, Germany. NICHD, NIH, Bethesda, MD 20892 USA. NIMH, NIH, Bethesda, MD 20892 USA. RP Haidan, A (reprint author), Univ Leipzig, Med Klin & Poliklin 3, Dept Internal Med 3, Philipp Rosenthal Str 27, D-04103 Leipzig, Germany. NR 38 TC 21 Z9 21 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1998 VL 19 IS 9 BP 1511 EP 1517 DI 10.1016/S0196-9781(98)00115-6 PG 7 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA 144TL UT WOS:000077331100006 PM 9864057 ER PT J AU Goodman, CB Heyliger, S Emilien, B Partilla, JS Yang, HYT Lee, CH Cadet, JL Rothman, RB AF Goodman, CB Heyliger, S Emilien, B Partilla, JS Yang, HYT Lee, CH Cadet, JL Rothman, RB TI Regulation of mu binding sites after chronic administration of antibodies directed against specific anti-opiate peptides SO PEPTIDES LA English DT Article DE NPFF; dynorphin; IgG; alpha-melanocyte-stimulating hormone (alpha-MSH) opioid mu receptor ID KAPPA-OPIOID RECEPTORS; MELANOCYTE-STIMULATING HORMONE; MORPHINE-INDUCED ANALGESIA; RAT-BRAIN; ANTIANALGESIC ACTION; DEPENDENCE; TOLERANCE; DELTA; DYNORPHIN; NPFF AB There is some indication that anti-opiate peptides (AOP) modulate opioid receptor systems by altering mu-receptor density. To further characterize this phenomenon, we investigated the effects of continuous infusion of anti-AOP IgG on mu binding sites in the brains of rats. Specifically, male Sprague-Dawley rats received intracerebroventricular (ICV) infusions for 13 days of either control (rabbit) IgG or test IgGs: anti-dynorphin A IgG, anti-dynorphin A(1-8) IgG, anti-alpha-MSH IgG, or the monoclonal anti-NPFF IgG. Administration of anti-NPFF IgG or the anti-dynorphin(1-8) IgG significantly increased mu labeling by 40-70% in several brain regions at the caudate level. Contrary to these findings, anti-alpha-MSH IgG decreased (19-32%) [I-125]-DAMGO labeling in several thalamic nuclei. The results suggest that the density of mu-opioid receptors is regulated in part by anti-opiate peptides in the extracellular fluid of the brain. (C) 1998 Elsevier Science Inc. C1 NIDA, DIR, NIH, Clin Psychopharmacol Sect, Baltimore, MD 21224 USA. NIDA, Mol Neuropsychiat Sect, Div IRP, Baltimore, MD 21224 USA. Florida A&M Univ, Coll Pharm & Pharmaceut Sci, Tallahassee, FL 32307 USA. US FDA, Lab Bacterial Polysaccharides, Div Bacterial Prod, OVRR,CBER, Bethesda, MD 20014 USA. RP Goodman, CB (reprint author), NIDA, DIR, NIH, Clin Psychopharmacol Sect, POB 5180, Baltimore, MD 21224 USA. EM cgoodman@famu.edu NR 50 TC 16 Z9 16 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1998 VL 19 IS 10 BP 1703 EP 1709 DI 10.1016/S0196-9781(98)00121-1 PG 7 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA 150XT UT WOS:000077691900009 PM 9880075 ER PT J AU Barker, JL Behar, T Li, YX Liu, QY Ma, W Maric, D Maric, I Schaffner, AE Serafini, R Smith, SV Somogyi, R Vautrin, JY Wen, XL Xian, H AF Barker, JL Behar, T Li, YX Liu, QY Ma, W Maric, D Maric, I Schaffner, AE Serafini, R Smith, SV Somogyi, R Vautrin, JY Wen, XL Xian, H TI GABAergic cells and signals in CNS development SO PERSPECTIVES ON DEVELOPMENTAL NEUROBIOLOGY LA English DT Article DE GAD; GABA; GABA(A) receptor subunits; proliferation; migration ID RAT SPINAL-CORD; GLUTAMIC-ACID DECARBOXYLASE; SUBUNIT MESSENGER-RNAS; CA3 HIPPOCAMPAL-NEURONS; CENTRAL-NERVOUS-SYSTEM; DORSAL HORN NEURONS; TRANSIENT EXPRESSION; POSTNATAL-DEVELOPMENT; GABA IMMUNOREACTIVITY; BRAIN AB GABA is formed primarily from decarboxylation of glutamate by a family of cytosolic and membrane-bound GAD enzymes. In the adult, GAD-derived GABA sustains the vitality of the central nervous system (CNS), since blockage of GAD rapidly leads to convulsions and death. In plants, cytosolic GAD synthesizes GABA in response to hormones and environmental stress. Since decarboxylation involves protonation, secretion of GABA serves to buffer cytosolic pH in plant cells. Families of GAD and GABA(A) receptor/Cl- channel transcripts and encoded proteins emerge early and seemingly everywhere during CNS development, with their abundance closely paralleling neurogenesis and peaking before birth. Micromolar GABA acts at receptor/Cl- channels to depolarize progenitor cells in the cortical neuroepithelium; it also elevates their cytosolic Ca2+ (Ca-C(2+)) levels. In some way, these effects decrease proliferation. GABA directs the migration of postmitotic neuroblasts at femtomolar concentrations and stimulates their random motility at micromolar concentrations via Ca2+ signaling mechanisms. Activation of GABA(A) receptors by micromolar GABA may limit motility via membrane depolarization and elevated Ca-C(2+). These results indicate that in vitro GABA can affect embryogenesis of the CNS through effects on cell proliferation and migration. As neurons differentiate postnatally, Cl--dependent depolarization disappears together with GABAergic CaCC2+ signals. Physiologically occurring GABAergic signals at Cl- channels exist in tonic and transient forms. Since the former are found on progenitor cells while both are present in postmitotic neurons, mechanisms to generate transients differentiate in the latter. Surprisingly, tonic and transient forms of GABAergic signaling at Cl- channels are rapidly and smoothly interconvertible and seem to be derived from online GABA synthesis in a surface-accessible compartment of the membrane. C1 NINDS, Neurophysiol Lab, Basic Neurosci Program, Div Intramural Res,NIH, Bethesda, MD 20892 USA. RP Barker, JL (reprint author), NINDS, Neurophysiol Lab, Basic Neurosci Program, Div Intramural Res,NIH, 36 Convent Dr,36-2C02 MSC4066, Bethesda, MD 20892 USA. NR 58 TC 126 Z9 132 U1 1 U2 4 PU GORDON BREACH SCI PUBL LTD PI READING PA C/O STBS LTD, PO BOX 90, READING RG1 8JL, BERKS, ENGLAND SN 1064-0517 J9 PERSPECT DEV NEUROBI JI Perspect. Dev. Neurobiol. PY 1998 VL 5 IS 2-3 BP 305 EP 322 PG 18 WC Developmental Biology; Neurosciences SC Developmental Biology; Neurosciences & Neurology GA 119YR UT WOS:000075926900018 PM 9777645 ER PT J AU Schwartz, JP Ji, Z Epelbaum, J AF Schwartz, JP Ji, Z Epelbaum, J TI Somatostatin as a neurotrophic factor - Which receptor/second messenger transduction system is involved? SO PERSPECTIVES ON DEVELOPMENTAL NEUROBIOLOGY LA English DT Article ID INSITU HYBRIDIZATION ANALYSIS; INFLUENCE NEURONAL EXPRESSION; HORMONE-RELEASING HORMONE; CEREBELLAR GRANULE CELLS; DEVELOPING CHICK BRAIN; RAT CEREBELLUM; IMMUNOREACTIVE NEURONS; GENE-EXPRESSION; CULTURED ASTROCYTES; NEURITE OUTGROWTH AB variety of studies support a trophic role for somatostatin in the developing nervous system, evidenced as stimulation of neurite outgrowth and axonal or neuronal migration in both in vivo and culture models. Cloning experiments have now demonstrated the existence of five subtypes of somatostatin receptor, differentially distributed in the nervous system, differentially linked to specific signal transduction systems and in certain cases differentially expressed during development. The combination of the differential and developmental regulation of expression of both the somatostatin peptides and their receptors thus provides great potential in terms of trophic effects. To sustantiate trophic effects of somatostatin, data are presented from two different model systems, cultures of cerebellar granule cells as well as transgenic mice in which somatostatin is expressed under the control of the glial fibrillary acidic protein promoter. Finally, potential receptor subtypes and second messenger systems involved in these trophic effects are addressed. C1 NINDS, Mol Genet Sect, Clin Neurosci Branch, NIH, Bethesda, MD 20892 USA. INSERM U159, Paris, France. RP Schwartz, JP (reprint author), NINDS, Mol Genet Sect, Clin Neurosci Branch, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA. RI Epelbaum, Jacques/B-2263-2013 NR 49 TC 22 Z9 22 U1 0 U2 0 PU GORDON BREACH SCI PUBL LTD PI READING PA C/O STBS LTD, PO BOX 90, READING RG1 8JL, BERKS, ENGLAND SN 1064-0517 J9 PERSPECT DEV NEUROBI JI Perspect. Dev. Neurobiol. PY 1998 VL 5 IS 4 BP 427 EP 435 PG 9 WC Developmental Biology; Neurosciences SC Developmental Biology; Neurosciences & Neurology GA 178UA UT WOS:000079284600007 PM 10533529 ER PT J AU Greenwald, MK Schuster, CR Johanson, CE Jewell, J AF Greenwald, MK Schuster, CR Johanson, CE Jewell, J TI Automated measurement of motor activity in human subjects: Effects of repeated testing and d-amphetamine SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Article DE humans; motor activity; exploratory behavior; time factors; habituation; amphetamine; central nervous system stimulants; drug abuse ID REST-ACTIVITY CYCLE; LOCOMOTOR-ACTIVITY; INDIVIDUAL VULNERABILITY; PSYCHOMOTOR PERFORMANCE; HEALTHY-VOLUNTEERS; DRUG PREFERENCE; MOTION SENSOR; OPEN-FIELD; DIAZEPAM; MOOD AB Repeated exposure to a test setting decreases, and amphetamine increases, motor activity in animals. To evaluate whether these effects also occur in human subjects, we recorded motor activity levels from 12 subjects during a double-blind oral drug discrimination (placebo vs. 75 mg tripelennamine) study. Before each 4-h session, activity monitors were attached to the subject's wrist and ankle. During each session, subjects rated their drug effects hourly (task periods), and could freely choose among leisure activities during intertask intervals (recreational periods). Habituation was evaluated by comparing activity response during initial (training phase) vs. later (discrimination phase) placebo sessions. During later sessions the two training drugs, as well as diazepam (2.5, 5 mg PO) and d-amphetamine (5, 10 mg PO) were administered. Consistent with animal studies, repeated exposure to the test environment significantly decreased, and d-amphetamine significantly and selectively increased, wrist motor activity. These data indicate that human motor activity is sensitive to environmental factors (task, time), drug class, and d-amphetamine dose. Activity measures may, therefore, be useful in evaluating environment/psychostimulant interactions in humans. (C) 1998 Elsevier Science Inc. C1 Natl Inst Drug Abuse, Addict Res Ctr, Div Intramural Res, Baltimore, MD 21224 USA. RP Greenwald, MK (reprint author), Wayne State Univ, Sch Med, Dept Psychiat & Behav Neurosci, Clin Res Div Subst Abuse, 2761 E Jefferson Ave, Detroit, MI 48207 USA. NR 41 TC 5 Z9 5 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD JAN PY 1998 VL 59 IS 1 BP 59 EP 65 DI 10.1016/S0091-3057(97)00387-0 PG 7 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA YN882 UT WOS:000071218100008 PM 9443537 ER PT J AU Li, XH Su, RTC Hsu, HT Sze, H AF Li, XH Su, RTC Hsu, HT Sze, H TI The molecular chaperone calnexin associates with the vacuolar H+-ATPase from oat seedlings SO PLANT CELL LA English DT Article ID PROTON-TRANSLOCATING ATPASE; ENDOPLASMIC-RETICULUM; SACCHAROMYCES-CEREVISIAE; SEQUENTIAL INTERACTION; MEMBRANE POLYPEPTIDE; SUBUNIT COMPOSITION; P-GLYCOPROTEIN; IN-VITRO; PROTEIN; COMPLEX AB Acidification of endomembrane compartments by the vacuolar-type H+-ATPase (V-ATPase) is central to many cellular processes in eukaryotes, including osmoregulation and protein sorting. The V-ATPase complex consists of a peripheral sector (V-1) and a membrane integral sector (V-o); however, it is unclear how the multimeric enzyme is assembled. A 64-kD polypeptide that had copurified with oat V-ATPase subunits has been identified as calnexin, an integral protein on the endoplasmic reticulum. To determine whether calnexin interacted physically with the V-ATPase, microsomal membranes were Triton X-100 solubilized, and the protein-protein interaction was analyzed by coimmunoprecipitation. Monoclonal antibodies against calnexin precipitated both calnexin and V-ATPase subunits, including A and B and those of 44, 42, 36, 16, and 13 kD. A monoclonal antibody against subunit A precipitated the entire V-ATPase complex as well as calnexin and Rip, an endoplasmic reticulum lumen chaperone. The results support our hypothesis that both calnexin and Dip act as molecular chaperones in the folding and assembly of newly synthesized V1Vo-ATPases at the endoplasmic reticulum. C1 Univ Maryland, Dept Plant Biol, College Pk, MD 20742 USA. NIH, Ctr Sci Review, Bethesda, MD 20892 USA. USDA ARS, Beltsville Agr Res Ctr, Florist & Nursery Crops Lab, Beltsville, MD 20705 USA. RP Sze, H (reprint author), Univ Maryland, Dept Plant Biol, College Pk, MD 20742 USA. EM hs29@umail.umd.edu NR 41 TC 36 Z9 38 U1 0 U2 1 PU AMER SOC PLANT PHYSIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 J9 PLANT CELL JI Plant Cell PD JAN PY 1998 VL 10 IS 1 BP 119 EP 130 PG 12 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA YV520 UT WOS:000071833300011 PM 9477575 ER PT J AU Ye, N Rogers, RD Brechbiel, MW Planalp, RP AF Ye, N Rogers, RD Brechbiel, MW Planalp, RP TI Synthesis and X-ray crystal structure of N,N ',N ''-tris(2-thienylmethyl)-cis-1,3,5-triaminocyclohexane copper(II) dichloride SO POLYHEDRON LA English DT Article DE copper complexes; square-pyramidal; nitrogen donor ligands ID MOLECULAR-STRUCTURE; LIGAND; HYDROLYSIS; COMPLEX; CIS,CIS-1,3,5-TRIAMINOCYCLOHEXANE AB The title complex was synthesized by reaction of N,N',N''-tris(2-thienylmethyl)-cis-1,3,5-triaminocyclohexane (tachthioph) with copper(II) chloride in acetonitrile or methanol. Crystallographic characterization of Cu(tachthioph)Cl-2.MeCN reveals a typical Jahn-Teller-distorted square-pyramidal CuN3Cl2 coordination sphere. There is no interaction with the 2-methylenethiophene pendant arms. (C) 1998 Elsevier Science Ltd. All rights reserved. C1 Univ New Hampshire, Dept Chem, Durham, NH 03824 USA. Univ Alabama, Dept Chem, Tuscaloosa, AL 35487 USA. NIH, Chem Sect, Radiat Oncol Branch, Bethesda, MD 20892 USA. RP Planalp, RP (reprint author), Univ New Hampshire, Dept Chem, Durham, NH 03824 USA. RI Rogers, Robin/C-8265-2013 OI Rogers, Robin/0000-0001-9843-7494 NR 11 TC 4 Z9 4 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0277-5387 J9 POLYHEDRON JI Polyhedron PY 1998 VL 17 IS 4 BP 603 EP 606 DI 10.1016/S0277-5387(97)00170-8 PG 4 WC Chemistry, Inorganic & Nuclear; Crystallography SC Chemistry; Crystallography GA YY103 UT WOS:000072113100028 ER PT J AU Cao, XL Tian, Y Zhang, TY Ito, Y AF Cao, XL Tian, Y Zhang, TY Ito, Y TI Semi-preparative separation and purification of taxol analogs by high-speed countercurrent chromatography SO PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY LA English DT Article AB High-speed countercurrent chromatography (HSCCC) was applied to the semi-preparative separation of taxol and its analogs, such as cephalomannine and 7-epi-10-deacetyltaxol from the extract of the bark of Taxus yunnannesis. The experiments were performed with a quaternary two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water through two steps. In the first step, the four components were separated into two groups at a volume ratio of 1:1:1:1 and, in the second step, two components in each group were separated at different volume ratios of 3:3:2:3 or 4:4:3:4. The present method also allows consecutive injections with reproducible results. C1 Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Cao, XL (reprint author), Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China. NR 6 TC 5 Z9 8 U1 2 U2 8 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6068 J9 PREP BIOCHEM BIOTECH JI Prep. Biochem. Biotechnol. PY 1998 VL 28 IS 1 BP 79 EP 87 DI 10.1080/10826069808010128 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA YZ565 UT WOS:000072266800006 PM 9516644 ER PT J AU Shibusawa, Y Ito, Y AF Shibusawa, Y Ito, Y TI Purification of proteins with aqueous two-phase solvent systems by countercurrent chromatography SO PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY LA English DT Article ID COIL PLANET CENTRIFUGE; LIQUID PARTITION CHROMATOGRAPHY; ELUTION CENTRIFUGE; SOLID SUPPORT; ROTARY SEALS; SEPARATION; LIPOPROTEINS; APPARATUS; SERUM C1 Tokyo Univ Pharm & Life Sci, Div Analyt Chem, Tokyo 19203, Japan. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Shibusawa, Y (reprint author), Tokyo Univ Pharm & Life Sci, Div Analyt Chem, 1432-1 Horinouchi, Tokyo 19203, Japan. NR 41 TC 3 Z9 3 U1 0 U2 2 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1082-6068 J9 PREP BIOCHEM BIOTECH JI Prep. Biochem. Biotechnol. PY 1998 VL 28 IS 2 BP 99 EP 136 DI 10.1080/10826069808010130 PG 38 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA ZQ359 UT WOS:000073853300001 PM 9608594 ER PT B AU Magruder, KM AF Magruder, KM BE Jenkins, R Ustun, TB TI Community education and screening programs SO PREVENTING MENTAL ILLNESS: MENTAL HEALTH PROMOTION IN PRIMARY CARE LA English DT Proceedings Paper CT Conference on Preventing Mental Illness - Mental Health Promotion in Primary Care CY JUL 12-14, 1995 CL LONDON, ENGLAND SP Dept Hlth, Royal Inst Public Hlth, WHO C1 NIMH, Rockville, MD 20857 USA. RP Magruder, KM (reprint author), NIMH, Rockville, MD 20857 USA. RI Jenkins, Rachel/E-4287-2010 OI Jenkins, Rachel/0000-0002-2958-0331 NR 3 TC 1 Z9 1 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, WEST SUSSEX, ENGLAND BN 0-471-97562-1 PY 1998 BP 171 EP 178 PG 8 WC Health Care Sciences & Services; Psychiatry; Psychology SC Health Care Sciences & Services; Psychiatry; Psychology GA BN25S UT WOS:000081338800017 ER PT B AU Heindel, JJ Collman, GW Suk, WA AF Heindel, JJ Collman, GW Suk, WA BE Kendall, RJ Dickerson, RL Giesy, JP Suk, WP TI Endocrine disruptors: extrapolation from wildlife to human effects SO PRINCIPLES AND PROCESSES FOR EVALUATING ENDOCRINE DISRUPTION IN SILDLIFE SE SETAC TECHNICAL PUBLICATIONS SERIES LA English DT Proceedings Paper CT Workshop on Principles and Processes for Evaluating Endocrine Disruption in Wildlife CY MAR, 1996 CL KIAWAH ISL, SC SP Natl Inst Environm Hlth Sci, Chem Manufacturers Assoc AB Wildlife and laboratory animal data suggest the possibility that endocrine-disrupting agents could pose a threat to human health. In the absence of conclusive data on the health effects of these agents in exposed human populations, risks due to exposure to endocrine disrupters must also rely on data from wildlife and laboratory animal studies and extrapolation of these data to humans. This extrapolation process can be made more precise if the animal studies and epidemiologic studies are designed and carried out with the principles of risk assessment in mind. The National Research Council (NRC 1983) put forward a risk assessment paradigm that is a useful framework to consider existing animal-and human-based evidence on the health effects of these chemicals. This paradigm includes hazard identification, dose-response assessment, exposure assessment, and risk characterization with the ultimate goal of impacting risk management decision-making, Different types of data are available from wildlife, laboratory-based animal and human tissue studies, and epidemiologic research that pertain to each of these categories, and the quality and quantity of these data will impact on the usefulness of risk assessment for endocrine-disrupter compounds (EDCs). C1 NIEHS, Res Triangle Pk, NC 27709 USA. RP Heindel, JJ (reprint author), NIEHS, POB 12233, Res Triangle Pk, NC 27709 USA. NR 0 TC 3 Z9 3 U1 2 U2 5 PU SETAC PRESS PI PENSACOLA PA 1010 N 12TH AVE, PENSACOLA, FL 32501 USA BN 1-880611-17-1 J9 SETAC TECH PUBLICAT PY 1998 BP 335 EP 347 PG 3 WC Environmental Sciences; Toxicology; Zoology SC Environmental Sciences & Ecology; Toxicology; Zoology GA BK89X UT WOS:000073834200015 ER PT S AU Brown, P Cervenakova, L McShane, L Kleihues, P Foncin, JF Collins, G Bastian, F Goldfarb, LG Gajdusek, DC AF Brown, P Cervenakova, L McShane, L Kleihues, P Foncin, JF Collins, G Bastian, F Goldfarb, LG Gajdusek, DC BE Morrison, DRO TI Polymorphic genotype matching in acquired Creutzfeldt-Jakob disease: An analysis of donor/recipient case pairs SO PRIONS AND BRAIN DISEASES IN ANIMALS AND HUMANS SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES LA English DT Proceedings Paper CT NATO Advanced Research Workshop on Prions and Brain Diseases in Animals and Humans CY AUG 19-23, 1996 CL ERICE, ITALY SP NATO C1 NINDS, CNS Studies Lab, NIH, Bethesda, MD 20892 USA. RP Brown, P (reprint author), NINDS, CNS Studies Lab, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0258-1213 BN 0-306-45825-X J9 NATO ADV SCI I A-LIF PY 1998 VL 295 BP 19 EP 24 PG 6 WC Biochemistry & Molecular Biology; Clinical Neurology; Neurosciences; Pathology SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pathology GA BK79P UT WOS:000073382500002 ER PT S AU Chesebro, B Priola, SA Race, RE AF Chesebro, B Priola, SA Race, RE BE Morrison, DRO TI Transgenic mice with neuron-specific expression of a hamster prion protein minigene are susceptible to hamster scrapie agent SO PRIONS AND BRAIN DISEASES IN ANIMALS AND HUMANS SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES LA English DT Proceedings Paper CT NATO Advanced Research Workshop on Prions and Brain Diseases in Animals and Humans CY AUG 19-23, 1996 CL ERICE, ITALY SP NATO C1 NIAID, Rocky Mt Labs, Lab Persistent Viral Dis, Hamilton, MT 59840 USA. RP Chesebro, B (reprint author), NIAID, Rocky Mt Labs, Lab Persistent Viral Dis, Hamilton, MT 59840 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0258-1213 BN 0-306-45825-X J9 NATO ADV SCI I A-LIF PY 1998 VL 295 BP 43 EP 47 PG 5 WC Biochemistry & Molecular Biology; Clinical Neurology; Neurosciences; Pathology SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pathology GA BK79P UT WOS:000073382500006 ER PT S AU Wickner, RB Masison, DC Edskes, H Maddelein, ML AF Wickner, RB Masison, DC Edskes, H Maddelein, ML BE Morrison, DRO TI Prions of yeast: Genetic evidence that the non-mendelian elements, [PSI] and [URE3] are altered self-replicating forms of SUP35p and URE2p, respectively SO PRIONS AND BRAIN DISEASES IN ANIMALS AND HUMANS SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES LA English DT Proceedings Paper CT NATO Advanced Research Workshop on Prions and Brain Diseases in Animals and Humans CY AUG 19-23, 1996 CL ERICE, ITALY SP NATO AB The word "prion" means infectious protein, an altered form of a cellular protein which may have lost its normal function, but has acquired the ability to convert the normal form of the protein into the same altered (prion) form. The yeast non-Mendelian genetic elements, [PSI] and [URE3], can be understood if they are viewed as prions arising from the normal proteins, Sup35p and Ure2p, respectively. Both [PSI] and [URE3] are a) reversibly curable, b) induced to arise by overproduction of the corresponding normal protein and c) depend upon their normal form chromosomal gene (SUP35 and URE2) for propagation and yet confer on the cell the same dominant phenotype as does a recessive mutation in this corresponding normal form chromosomal gene. Our 'yeast prion hypotheses' have now been supported by biochemical data indicating that Sup35p is altered in [PSI] strains and Ure2p is altered in [URE3] strains. We have defined a prion-inducing domain of Ure2p, an asparagine - rich N-terminal region which mediates the prion alteration in cis and in trans. C1 NIDDKD, Lab Biochem & Genet, NIH, Bethesda, MD 20892 USA. RP Wickner, RB (reprint author), NIDDKD, Lab Biochem & Genet, NIH, Bldg 8,Room 225,8 Ctr Dr,MSC 0830, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0258-1213 BN 0-306-45825-X J9 NATO ADV SCI I A-LIF PY 1998 VL 295 BP 111 EP 121 PG 11 WC Biochemistry & Molecular Biology; Clinical Neurology; Neurosciences; Pathology SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pathology GA BK79P UT WOS:000073382500012 ER PT S AU Caughey, B Raymond, GJ AF Caughey, B Raymond, GJ BE Morrison, DRO TI Protease-resistant prion protein formation SO PRIONS AND BRAIN DISEASES IN ANIMALS AND HUMANS SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES LA English DT Proceedings Paper CT NATO Advanced Research Workshop on Prions and Brain Diseases in Animals and Humans CY AUG 19-23, 1996 CL ERICE, ITALY SP NATO C1 NIAID, Rocky Mt Labs, Persistent Viral Dis Lab, NIH, Hamilton, MT 59840 USA. RP Caughey, B (reprint author), NIAID, Rocky Mt Labs, Persistent Viral Dis Lab, NIH, Hamilton, MT 59840 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0258-1213 BN 0-306-45825-X J9 NATO ADV SCI I A-LIF PY 1998 VL 295 BP 217 EP 224 PG 8 WC Biochemistry & Molecular Biology; Clinical Neurology; Neurosciences; Pathology SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pathology GA BK79P UT WOS:000073382500022 ER PT B AU Underwood, BA AF Underwood, BA BE Fitzpatrick, DW Anderson, JE LAbbe, ML TI Micronutrient malnutrition: Is it being eliminated? SO PROCEEDINGS OF THE 16TH INTERNATIONAL CONGRESS OF NUTRITION - NUTRITION MONTREAL 97: FROM NUTRITIONAL SCIENCE TO NUTRITION PRACTICE FOR BETTER GLOBAL HEALTH LA English DT Proceedings Paper CT 16th International Congress of Nutrition CY JUL 27-AUG 01, 1997 CL MONTREAL, CANADA SP Nat Res Council Canada, Agr Inst Canada, Canadian Inst Food Sci & Technol, Canadian Soc Nutr Sci, Dietitians Canada, Int Union Nutr Sci DE micronutrients; iron; iodine; vitamin A ID VITAMIN-A C1 NEI, NIH, Bethesda, MD 20892 USA. RP Underwood, BA (reprint author), NEI, NIH, Bethesda, MD 20892 USA. NR 13 TC 0 Z9 0 U1 0 U2 0 PU CANADIAN FEDERATION BIOLOGICALSOCIETIES PI OTTAWA PA OTTAWA, ON, CANADA PY 1998 BP 4 EP 6 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA BP22W UT WOS:000084442600002 ER PT B AU Lieberman, R AF Lieberman, R BE Anderson, JG Katzper, M TI Simulation modeling of intermediate efficacy endpoints monitoring in cancer prevention and treatment trials using Bayesian principles SO PROCEEDINGS OF THE 1998 MEDICAL SCIENCES SIMULATION CONFERENCE LA English DT Proceedings Paper CT Medical Sciences Simulation Conference held at the 1998 Western MultiConference CY JAN 11-14, 1998 CL SAN DIEGO, CA SP Soc Comp Simulat Int AB We have investigated through simulation models accumulating data from hypothetical randomized placebo: controlled trials based on literature derived values (mean and variance) for the: primary response variable, e.g., intermediate efficacy endpoints (IEE), the performance of a Bayesian-styled sequential monitoring strategy with predefined stopping rules. A sensitivity analysis derived from 50 thousand randomized controlled trials with a sample size of 100 subjects per trial in which the IEE and variance was allowed to change (+/-50%) creating multiple scenarios showed that the Bayesian method was the quickest to stop for ineffective agents and very slow to stop for active agents compared to standard group sequential methods (Popcock, O'Brien-Fleming). In summary, the combination of flexibility, strong performance and ease-of interpretation of the data conferred by the Bayesian approach suggests that it is well suited for IEE guided trials and can be applied to ongoing randomized cancer prevention and trreatment trials for rapid identification of either ineffective or promising agents using planned interim analyses with small sample sizes. C1 NCI, Div Canc Prevent, Bethesda, MD 20892 USA. RP Lieberman, R (reprint author), NCI, Div Canc Prevent, Bethesda, MD 20892 USA. NR 4 TC 0 Z9 0 U1 0 U2 0 PU SOC COMPUTER SIMULATION PI SAN DIEGO PA PO BOX 17900, SAN DIEGO, CA 92177 USA BN 1-56555-138-9 PY 1998 BP 140 EP 142 PG 3 WC Computer Science, Interdisciplinary Applications; Medical Informatics SC Computer Science; Medical Informatics GA BN53S UT WOS:000082161100023 ER PT J AU Humphrey, SM AF Humphrey, SM TI A new approach to automatic indexing using journal descriptors SO PROCEEDINGS OF THE ASIS ANNUAL MEETING LA English DT Article; Proceedings Paper CT 61st ASIS Annual Meeting CY OCT 24-29, 1998 CL PITTSBURGH, PENNSYLVANIA SP Amer Soc Informat Sci (ASIS) C1 Natl Lib Med, Bethesda, MD 20209 USA. RP Humphrey, SM (reprint author), Natl Lib Med, Bethesda, MD 20209 USA. NR 5 TC 1 Z9 1 U1 0 U2 0 PU INFORMATION TODAY INC PI MEDFORD PA 143 OLD MARLTON PIKE, MEDFORD, NJ 08055-8750 USA SN 0044-7870 J9 P ASIS ANNU MEET JI Proc. ASIS Annu. Meet. PY 1998 VL 35 BP 496 EP 500 PG 5 WC Computer Science, Information Systems; Information Science & Library Science SC Computer Science; Information Science & Library Science GA 149BM UT WOS:000077585300048 ER PT J AU Chanock, RM AF Chanock, RM TI Albert Bruce Sabin 1906-1993 SO PROCEEDINGS OF THE ASSOCIATION OF AMERICAN PHYSICIANS LA English DT Biographical-Item C1 NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. RP Chanock, RM (reprint author), NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 1081-650X J9 P ASSOC AM PHYSICIAN JI Proc. Assoc. Am. Phys. PD JAN-FEB PY 1998 VL 110 IS 1 BP 84 EP 86 PG 3 WC Medicine, General & Internal; Medicine, Research & Experimental SC General & Internal Medicine; Research & Experimental Medicine GA YR128 UT WOS:000071461100013 ER PT J AU Weisburger, JH Rivenson, A Aliaga, C Reinhardt, J Kelloff, GJ Boone, CW Steele, VE Balentine, DA Pittman, B Zang, E AF Weisburger, JH Rivenson, A Aliaga, C Reinhardt, J Kelloff, GJ Boone, CW Steele, VE Balentine, DA Pittman, B Zang, E TI Effect of tea extracts, polyphenols, and epigallocatechin gallate on azoxymethane-induced colon cancer SO PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE LA English DT Article ID GREEN TEA; BLACK TEA; F344 RAT AB Studies were conducted to determine the chemopreventive efficacy of several types of tea extracts on azoxymethane-induced colon cancer in male F344 rats, After determining the maximally tolerated dosage of the tea products, their effect in a colon cancer model was investigated, Groups of 36 male F344 rats received 2 subcutaneous doses of 15 mg/kg azoxymethane (AOM) at Weeks 6 and 7, Experimental groups also received as drinking fluids 3600 ppm of black or green tea extracts, 1800 ppm of EGCG, or 1800 ppm of black or green tea polyphenols beginning at 5 weeks of age, Additional groups drank a lower dose of 360 ppm of the five tea products. The experiments were terminated 43 weeks after the first tea exposure, No evidence of toxicity was observed since the body weight gain of all groups was similar, The rats given AOM had carcinoma of the small intestine and of the colon, classified histologically as in situ carcinoma, exophytic, invasive, and Peyer's patch carcinoma, In the small intestine, most of the neoplasms were classified as invasive, but in the colon, most were exophytic. The various tea products failed to produce a significant difference in the incidence of the several types of colon and small intestine carcinoma. The multiplicity of colon cancers ranged from 1.2-2.8 in all groups, The group on 3600 ppm of green tea had a significantly higher tumor multiplicity than the control group on AOM and water, Also, the group on 3600 ppm of green tea had a significantly higher tumor multiplicity than the group on 360 ppm, The tea products did not affect the development aspects of the tumors in most groups. The mechanisms underlying these findings rest on the fact that azoxymethane is metabolized mainly by cytochrome P450 2E1, and this enzyme system is not affected by tea. C1 Amer Hlth Fdn, Naylor Dana Inst, Valhalla, NY 10595 USA. NCI, Div Canc Prevent & Control, Bethesda, MD 20892 USA. Thomas J Lipton Co, Englewood Cliffs, NJ 07632 USA. RP Weisburger, JH (reprint author), Amer Hlth Fdn, Naylor Dana Inst, 1 Dana Rd, Valhalla, NY 10595 USA. FU NCI NIH HHS [N01-CN-35569] NR 19 TC 40 Z9 40 U1 0 U2 3 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0037-9727 J9 P SOC EXP BIOL MED JI Proc. Soc. Exp. Biol. Med. PD JAN PY 1998 VL 217 IS 1 BP 104 EP 108 PG 5 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA YM416 UT WOS:000071061300015 PM 9421213 ER PT J AU Venkatesh, B Manoharan, PT Rifkind, JM AF Venkatesh, B Manoharan, PT Rifkind, JM TI Metal ion reconstituted hybrid hemoglobins SO PROGRESS IN INORGANIC CHEMISTRY, VOL 47 SE PROGRESS IN INORGANIC CHEMISTRY LA English DT Review ID NUCLEAR-MAGNETIC-RESONANCE; ELECTRON-PARAMAGNETIC-RESONANCE; OXYGEN EQUILIBRIUM PROPERTIES; HEME-HEME INTERACTION; PARTIALLY LIGANDED HEMOGLOBIN; CARBON-MONOXIDE BINDING; PERFORMANCE LIQUID-CHROMATOGRAPHY; RESOLVED CIRCULAR-DICHROISM; ABSORPTION FINE-STRUCTURE; COOPERATIVE FREE-ENERGIES C1 Indian Inst Technol, Reg Sophisticated Instrumentat Ctr, Dept Chem, Madras 600036, Tamil Nadu, India. Natl Inst Hlth, Mol Dynam Sect, Lab Cellular Mol Biol, Baltimore, MD USA. Natl Inst Hlth, Mol Dynam Sect, Inst Aging Mol Biol, Baltimore, MD USA. RP Venkatesh, B (reprint author), Indian Inst Technol, Reg Sophisticated Instrumentat Ctr, Dept Chem, Madras 600036, Tamil Nadu, India. NR 331 TC 13 Z9 13 U1 2 U2 2 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 3RD AVE, NEW YORK, NY 10016 USA SN 0079-6379 J9 PROG INORG CHEM PY 1998 VL 47 BP 563 EP 684 PG 124 WC Chemistry, Inorganic & Nuclear SC Chemistry GA BK55Z UT WOS:000072568600006 ER PT J AU Flanders, KC Ren, RF Lippa, CF AF Flanders, KC Ren, RF Lippa, CF TI Transforming growth factor-beta s in neurodegenerative disease SO PROGRESS IN NEUROBIOLOGY LA English DT Review ID AMYLOID PRECURSOR PROTEIN; CENTRAL-NERVOUS-SYSTEM; VENTRICULAR CEREBROSPINAL-FLUID; MIDBRAIN DOPAMINERGIC-NEURONS; TRANSIENT FOREBRAIN ISCHEMIA; TGF-BETA-1 MESSENGER-RNA; RAT HIPPOCAMPAL-NEURONS; TGF-BETA; ALZHEIMERS-DISEASE; TRANSGENIC MICE AB Transforming growth factors-beta s (TGF-beta s), a family of multifunctional peptide growth factors, affect cells of the central nervous system (CNS). The three mammalian TGF-beta isoforms, TGF-beta s 1, 2 and 3, are expressed in adult human brain. Since neuronal degeneration is a defining feature of CNS degenerative diseases, TGF-beta may be important because it can influence neuronal survival. In vitro TGF-beta promotes survival of rat spinal cord motoneurons and dopaminergic neurons. In addition to direct effects on neuronal survival, TGF-beta treatment of cultured astrocytes induces a reactive phenotype. Thus, TGF-beta may also normalize the extracellular matrix environment in degenerative diseases. The expression of TGF-beta s change in response to neuronal injury. TGF-beta 1 expression increases in astrocytes and microglia in animal models of cerebral ischemia, while TGF-beta 2 expression increases in activated astroglial cells in human neurodegenerative diseases. TGF-beta s protect neurons from a variety of insults. TGF-beta maintains survival of chick telencephalic neurons made hypoxic by treatment with cyanide and decreases the area of infarction when administered in animal models of cerebral ischemia. In vitro TGF-beta protects neurons from damage induced by treatment with beta-amyloid peptide, FeSO4 (induces production of reactive oxygen species), Ca2+ ionophores, glutamate, glutamate receptor agonists and MPTP (toxic for dopaminergic neurons). TGF-beta maintains mitochondrial potential and Ca2+ homeostasis and inhibits apoptosis in neurons. TGF-beta does not prevent neuronal degeneration in a rat model of Parkinson's disease and has yet to be tested in newly developed transgenic mouse models of Alzheimer's disease. TGF-beta is a potent neuroprotective agent which may affect the pathogenesis of neurodegenerative diseases of the CNS. (C) 1997 Elsevier Science Ltd. C1 NCI, Chemoprevent Lab, Bethesda, MD 20892 USA. Allegheny Univ Hlth Sci, MCP Div, Dept Neurol, Philadelphia, PA 19129 USA. RP Flanders, KC (reprint author), NCI, Chemoprevent Lab, Bldg 41,Room C-629, Bethesda, MD 20892 USA. EM FLANDERK@DCE41.NCI.NIH.GOV NR 99 TC 272 Z9 285 U1 1 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0301-0082 J9 PROG NEUROBIOL JI Prog. Neurobiol. PD JAN PY 1998 VL 54 IS 1 BP 71 EP 85 DI 10.1016/S0301-0082(97)00066-X PG 15 WC Neurosciences SC Neurosciences & Neurology GA YQ294 UT WOS:000071370900005 PM 9460794 ER PT J AU Wolffe, AP Kurumizaka, H AF Wolffe, AP Kurumizaka, H TI The nucleosome: A powerful regulator of transcription SO PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 61 SE PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY LA English DT Review ID RIBOSOMAL-RNA GENE; HIGHER-ORDER STRUCTURE; TUMOR VIRUS PROMOTER; CO-CRYSTAL STRUCTURE; LINKER HISTONES B4; GLOBULAR DOMAIN; XENOPUS-LAEVIS; IN-VIVO; GLUCOCORTICOID RECEPTOR; VITELLOGENIN GENE C1 NICHHD, Mol Embryol Lab, NIH, Bethesda, MD 20892 USA. RP Wolffe, AP (reprint author), NICHHD, Mol Embryol Lab, NIH, Bethesda, MD 20892 USA. NR 166 TC 80 Z9 80 U1 3 U2 6 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0079-6603 J9 PROG NUCLEIC ACID RE PY 1998 VL 61 BP 379 EP 422 DI 10.1016/S0079-6603(08)60832-6 PG 44 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BM67V UT WOS:000079436100010 PM 9752726 ER PT J AU Santos, AD Padlan, EA AF Santos, AD Padlan, EA TI Development of more efficacious antibodies for medical therapy and diagnosis SO PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOLUME 60 SE PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY LA English DT Review ID RESHAPING HUMAN-ANTIBODIES; ANTIGEN-BINDING; HYPERVARIABLE REGIONS; BISPECIFIC ANTIBODY; VARIABLE DOMAINS; ESCHERICHIA-COLI; IMMUNOGLOBULIN-E; FV FRAGMENTS; BIVALENT; MOUSE C1 NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. Univ Philippines, Natl Inst Chem, Quezon 1101, Philippines. RP Santos, AD (reprint author), NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. NR 57 TC 3 Z9 3 U1 2 U2 3 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0079-6603 J9 PROG NUCLEIC ACID RE PY 1998 VL 60 BP 169 EP 194 DI 10.1016/S0079-6603(08)60893-4 PG 26 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BK97R UT WOS:000073976600007 PM 9594575 ER PT J AU Schulkin, J AF Schulkin, J TI Fear and its neuroendocrine basis SO PROGRESS IN PSYCHOBIOLOGY AND PHYSIOLOGICAL PSYCHOLOGY, VOL 17 SE PROGRESS IN PSYCHOBIOLOGY AND PHYSIOLOGICAL PSYCHOLOGY LA English DT Review ID CORTICOTROPIN-RELEASING-FACTOR; CENTRAL AMYGDALOID NUCLEUS; HYPOTHALAMIC PARAVENTRICULAR NUCLEUS; POSTTRAUMATIC-STRESS-DISORDER; FACTOR-LIKE IMMUNOREACTIVITY; ACOUSTIC STARTLE REFLEX; HORMONE MESSENGER-RNA; URINARY-FREE CORTISOL; DORSAL MOTOR NUCLEUS; BEHAVIORAL-INHIBITION C1 Georgetown Univ, Dept Physiol & Biophys, NIMH, Clin Neuroendocrinol Branch,Behav Neurosci Unit, Washington, DC 20007 USA. RP Schulkin, J (reprint author), Georgetown Univ, Dept Physiol & Biophys, NIMH, Clin Neuroendocrinol Branch,Behav Neurosci Unit, Washington, DC 20007 USA. NR 212 TC 2 Z9 2 U1 2 U2 3 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0363-0951 J9 PROG PSYCHOB PHYSIOL PY 1998 VL 17 BP 35 EP 66 PG 32 WC Psychology SC Psychology GA BM62P UT WOS:000079286900002 ER PT J AU Jorcyk, CL Liu, ML Shibata, MA Maroulakou, IG Komschlies, KL McPhaul, MJ Resau, JH Green, JE AF Jorcyk, CL Liu, ML Shibata, MA Maroulakou, IG Komschlies, KL McPhaul, MJ Resau, JH Green, JE TI Development and characterization of a mouse prostate adenocarcinoma cell line: Ductal formation determined by extracellular matrix SO PROSTATE LA English DT Article DE SV40; transgenic mice; prostate cancer; extracellular matrix; transformation ID EPITHELIAL-CELLS; BASEMENT-MEMBRANES; TRANSGENIC MICE; GROWTH-FACTOR; RAT PROSTATE; SERUM-FREE; CULTURE; CARCINOMA; ANDROGEN; DIFFERENTIATION AB BACKGROUND. Tumor vaccines show promise as a new approach for treating cancer. We have developed a murine prostate cancer cell line which can be used to study growth factor and extracellular matrix regulation of prostate differentiation and will be useful for generating tumor vaccines using the C3(1)/T-AG transgenic model of prostate cancer. METHODS. Pr-14 cells were established in defined growth media (GM) and grown in GM, GM + 2% fetal bovine serum (FBS) or DMEM + 10% FBS on plastic, collagen, or Matrigel. Immunofluorescence and Western blot analyses were performed using antibodies to cytokeratin, vimentin, SV40 large T-antigen, and androgen receptor (BR). RESULTS. Pr-14 cells are cytokeratin-positive, vimentin-negative, and express SV40 large T-antigen. These cells are tumorigenic when injected into athymic nude mice and appear to be androgen-independent. Pr-14 cell lines are nontumorigenic when injected into syngeneic FVB/N mice, but form tumors in transgenic T-AG-expressing FVB/N mice. Cell growth and morphology are dependent on media composition which determines whether ductal or acinar structures form when grown on Matrigel. CONCLUSIONS. The mouse prostate adenocarcinoma cell line, Pr-14, undergoes alterations in the state of differentiation dependent upon serum concentration when grown on Matrigel. The Pr-14 cell line is a useful reagent to study prostate cell/extracellular matrix interactions, and for immunotherapy and cancer vaccine studies in C3(1)/T-AG transgenic mice. (C) 1998 Wiley-Liss, Inc. C1 NCI, Chemoprevent Lab, Bethesda, MD 20892 USA. NCI, SAIC, Frederick Canc Res & Dev Ctr, Frederick, MD 21701 USA. Univ Texas, SW Med Ctr, Dept Surg, Div Urol, Dallas, TX 75235 USA. NCI, ABL Basic Res Program, FCRDC, Frederick, MD 21701 USA. RP Green, JE (reprint author), NCI, Chemoprevent Lab, Bldg 41,Room C619, Bethesda, MD 20892 USA. NR 38 TC 16 Z9 16 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0270-4137 J9 PROSTATE JI Prostate PD JAN 1 PY 1998 VL 34 IS 1 BP 10 EP 22 PG 13 WC Endocrinology & Metabolism; Urology & Nephrology SC Endocrinology & Metabolism; Urology & Nephrology GA YN762 UT WOS:000071204000002 PM 9428383 ER PT S AU Gronenborn, AM Clore, GM AF Gronenborn, AM Clore, GM BE Jardetzky, O Lefevre, JF TI Determining structures of protein/DNA complexes by NMR SO PROTEIN DYNAMICS, FUNCTION, AND DESIGN SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES LA English DT Proceedings Paper CT NATO Advanced Study Institute and International School of Structural Biology and Magnetic Resonance, 3rd Course on Protein Dynamics. Function, and Design CY APR 16-28, 1997 CL ERICE, ITALY SP NATO ID DNA-BINDING DOMAIN; MAGNETIC-RESONANCE SPECTROSCOPY; CRYSTAL-STRUCTURE; DIRECT REFINEMENT; IMPROVED SENSITIVITY; SEX DETERMINATION; CHEMICAL-SHIFTS; MINOR-GROOVE; ZINC FINGERS; GAGA FACTOR C1 NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. RP Gronenborn, AM (reprint author), NIDDKD, Chem Phys Lab, NIH, Bldg 2, Bethesda, MD 20892 USA. NR 61 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0258-1213 BN 0-306-45939-6 J9 NATO ADV SCI I A-LIF PY 1998 VL 301 BP 1 EP 13 PG 13 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Spectroscopy SC Biochemistry & Molecular Biology; Biophysics; Spectroscopy GA BM32J UT WOS:000078390000001 ER PT J AU Caton, CLM Cournos, F Felix, A Wyatt, RJ AF Caton, CLM Cournos, F Felix, A Wyatt, RJ TI Childhood experiences and current adjustment of offspring of indigent patients with schizophrenia SO PSYCHIATRIC SERVICES LA English DT Article; Proceedings Paper CT 149th Annual Meeting of the American-Psychiatric-Association CY MAY 04-09, 1996 CL NEW YORK, NEW YORK SP Amer Psychiat Assoc ID SERIOUS MENTAL-ILLNESS; YOUNG-CHILDREN; RISK-FACTORS; WOMEN; DISORDERS; HOMELESSNESS; PREGNANCY; MOTHERS; PARENTS AB Objective: This study reports the childhood experiences, current life situation and level of adjustment, and prior mental health service use of offspring of indigent people with schizophrenia. Methods: Sixty-eight patient-parents were asked for consent for researchers to contact their adolescent and adult offspring. Thirty-nine consenting offspring were interviewed with an assessment battery that included measures of current occupational and social functioning, psychiatric status, and mental health service use. Results: Interviewed offspring were raised in an average of three different settings from birth to 18 years of age. Relatives, particularly grandparents and aunts, were more likely to provide surrogate parenting than were nonkin foster parents and were more significant nurturing figures than biological parents. The typical offspring had a high school diploma, was gainfully employed, and was involved with a spouse or household partner or had a close friend. Twenty-three of the 39 offspring had children, and most were raising their children alone. Ten offspring had a diagnosis of major depression, schizoaffective disorder, or drug or alcohol abuse, but none had a diagnosis of schizophrenia. Four of the ten offspring with a psychiatric diagnosis had never been treated. Conclusions: Findings underscore the need for long-term studies of families with a parent who is a psychiatric patient. Rehabilitation efforts should include extended family who play a critical role in raising offspring during periods when patient-parents are unable to do so. Offspring should be included in efforts to educate families about schizophrenia. C1 Columbia Univ Coll Phys & Surg, Dept Psychiat, New York, NY 10032 USA. NIMH, St Elizabeths Hosp, Neuropsychiat Branch, Washington, DC 20032 USA. RP Caton, CLM (reprint author), Columbia Univ Coll Phys & Surg, Dept Psychiat, 722 W 168th St, New York, NY 10032 USA. NR 29 TC 23 Z9 24 U1 0 U2 2 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 USA SN 1075-2730 J9 PSYCHIATR SERV JI Psychiatr. Serv. PD JAN PY 1998 VL 49 IS 1 BP 86 EP 90 PG 5 WC Health Policy & Services; Public, Environmental & Occupational Health; Psychiatry SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Psychiatry GA YQ973 UT WOS:000071443800013 PM 9444686 ER PT J AU McCrae, RR AF McCrae, RR TI An empirically based alternative framework SO PSYCHOLOGICAL INQUIRY LA English DT Editorial Material ID 5-FACTOR MODEL C1 NIA, Personal Stress & Coping Sect, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA. RP McCrae, RR (reprint author), NIA, Personal Stress & Coping Sect, Gerontol Res Ctr, NIH, 4940 Eastern Ave, Baltimore, MD 21224 USA. NR 12 TC 0 Z9 0 U1 0 U2 0 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 USA SN 1047-840X J9 PSYCHOL INQ JI Psychol. Inq. PY 1998 VL 9 IS 2 BP 158 EP 160 DI 10.1207/s15327965pli0902_16 PG 3 WC Psychology, Multidisciplinary SC Psychology GA ZW857 UT WOS:000074455300016 ER PT J AU Zahn-Waxler, C Klimes-Dougan, B Kendziora, KT AF Zahn-Waxler, C Klimes-Dougan, B Kendziora, KT TI The study of emotion socialization: Conceptual, methodological, and developmental considerations SO PSYCHOLOGICAL INQUIRY LA English DT Article ID DISORDERS C1 NIMH, Dev Psychopathol Sect, Bethesda, MD 20892 USA. RP Zahn-Waxler, C (reprint author), NIMH, Dev Psychopathol Sect, Room 204-A,Bldg 15-K,9000 Rockville Pike, Bethesda, MD 20892 USA. NR 15 TC 9 Z9 9 U1 1 U2 2 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 USA SN 1047-840X J9 PSYCHOL INQ JI Psychol. Inq. PY 1998 VL 9 IS 4 BP 313 EP 316 DI 10.1207/s15327965pli0904_16 PG 4 WC Psychology, Multidisciplinary SC Psychology GA 183LG UT WOS:000079554600016 ER PT J AU Mele, A Wozniak, KM Hall, FS Pert, A AF Mele, A Wozniak, KM Hall, FS Pert, A TI The role of striatal dopaminergic mechanisms in rotational behavior induced by phencyclidine and phencyclidine-like drugs SO PSYCHOPHARMACOLOGY LA English DT Review DE phencyclidine; rotational behavior; striatal dopamine; rat ID D-ASPARTIC ACID; NMDA RECEPTOR ANTAGONIST; RAT PREFRONTAL CORTEX; GUINEA-PIG BRAIN; INCREASES EXTRACELLULAR DOPAMINE; INDUCED LOCOMOTOR-ACTIVITY; MONOAMINE-DEPLETED MICE; AFFINITY BINDING-SITES; NUCLEUS-ACCUMBENS; INVIVO MICRODIALYSIS AB Phencyclidine (PCP) and phencyclidine-like drugs (TCP, dexoxadrol, MK-801, and SKF 10,047) were evaluated for their ability to induce rotational behavior in rats with unilateral 6-OHDA lesions of the medial forebrain bundle and for their ability to alter striatal dopamine (DA) overflow with microdialysis procedures. All of the compounds tested produced rotational behavior ipsilateral to the lesion, suggesting that they were enhancing extracellular dopamine in the intact striatum. The microdialysis studies, however, did not support this contention. There appeared to be a complete dissociation between the ability of the five compounds to produce ipsilateral rotations and their ability to enhance extracellular dopamine levels in the striatum. PCP was the only compound able to elicit significant increases in striatal dopamine overflow following IP injections and also produce dramatic rotational behavior. MK-801 was the most potent compound in enhancing rotational output while it had no effect at all on striatal dopamine overflow. Dexoxadrol also produced significant rotational output without having any effect on extracellular levels of dopamine following IP injections. TCP and SKF 10,047, at doses which produced significant rotational behavior, only elevated dopamine 16% and 12%, respectively, at peak effect. It is most parsimonious to conclude that the effects of PCP-like drugs on nigro-striatal function are mediated through their ability to act as indirect NMDA receptor antagonists and not through their ability to alter striatal dopamine activity. C1 NIMH, Biol Psychiat Branch, NIH, Bethesda, MD 20892 USA. NIAAA, Clin Studies Lab, Bethesda, MD 20892 USA. RP Pert, A (reprint author), NIMH, Biol Psychiat Branch, NIH, Bethesda, MD 20892 USA. RI Mele, Andrea/E-7741-2015; Hall, Frank/C-3036-2013 OI Hall, Frank/0000-0002-0822-4063 NR 110 TC 12 Z9 12 U1 0 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD JAN PY 1998 VL 135 IS 2 BP 107 EP 118 DI 10.1007/s002130050491 PG 12 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA YU959 UT WOS:000071773800001 PM 9497015 ER PT J AU Litten, RZ Allen, JP AF Litten, RZ Allen, JP TI Pharmacologic treatment of alcoholics with collateral depression: Issues and future directions SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article DE medications; alcoholism; depression; comorbidity; alcoholism treatment ID MAJOR DEPRESSION; USE DISORDERS; IMIPRAMINE; SEVERITY; TRIAL AB Patients in treatment for alcoholism often suffer collateral depressive disorders. Fortunately, recent advances in medications development may significantly improve outcome for this population, but there remain several concerns about the clinical use of pharmacologic agents in these cases. These concerns are discussed, as are research findings that bear on them. Directions for future research are also identified. C1 NIAAA, Treatment Res Branch, Bethesda, MD 20892 USA. RP Litten, RZ (reprint author), NIAAA, Treatment Res Branch, Willco Bldg,Suite 505,6000 Execut Blvd, Bethesda, MD 20892 USA. NR 23 TC 11 Z9 11 U1 3 U2 3 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPERINTENDENT DOCUMENTS,, WASHINGTON, DC 20402-9325 USA SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1998 VL 34 IS 1 BP 107 EP 110 PG 4 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA 161PE UT WOS:000078297000017 PM 9564206 ER PT J AU Blehar, MC Rudorfer, MV AF Blehar, MC Rudorfer, MV TI Women's mental health research - What is the need? SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material C1 NIMH, Div Mental Disorders Behav Res & AIDS, Rockville, MD 20857 USA. RP Blehar, MC (reprint author), NIMH, Div Mental Disorders Behav Res & AIDS, Rockville, MD 20857 USA. NR 2 TC 1 Z9 1 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPERINTENDENT DOCUMENTS,, WASHINGTON, DC 20402-9325 USA SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1998 VL 34 IS 3 BP 237 EP 238 PG 2 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA 161PH UT WOS:000078297300002 PM 9803747 ER PT J AU Blehar, MC DePaulo, JR Gershon, ES Reich, T Simpson, SG Nurnberger, JI AF Blehar, MC DePaulo, JR Gershon, ES Reich, T Simpson, SG Nurnberger, JI TI Women with bipolar disorder: Findings from the NIMH genetics initiative sample SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article DE bipolar disorder; gender differences; postpartum; menopause; premenstrual exacerbation; genetics; family studies ID HYPOTHYROIDISM; PSYCHOSES AB Bipolar l (BPl) mood disorder is a severe recurrent mental illness with a population prevalence of 1 percent. Evidence is strong for genetic risk factors in onset. However, unlike unipolar mood disorders, in which women outnumber men by 2 to 1, for BPl disorder, the male:female ratio is equal. Perhaps for this reason, relatively little research has examined gender-related risks in BPl course, This article presents data from 186 BPl women and 141 BPl men ascertained as part of the NIMH Genetics initiative, a multisite collaborative molecular genetic study. Subjects were interviewed using the Diagnostic Interview for Genetic Studies (DIGS), DIGS items included a medical history, and for women, questions concerning psychiatric disorders in relation to childbearing, the menstrual cycle, and menopause. Almost half of BPl women who had been pregnant reported having experienced severe emotional disturbances in relation to childbearing, with close to one third reporting episode onset during pregnancy. Two-thirds of BPl women reported frequent premenstrual mood disturbances and almost 20 percent of postmenopausal BPl women reported severe emotional disturbances during the menopausal transition. More BPl women than men reported thyroid disorder and migraine headaches. Findings are discussed in relation to gender differences in population and other clinical samples, and in terms of their implications for the development of new treatments and preventive Interventions. C1 NIMH, Schizophrenia Mood & Other Brain Disorders Progra, DMBDA, Rockville, MD 20857 USA. Johns Hopkins Univ, Dept Psychiat, Baltimore, MD USA. Univ Chicago, Dept Psychiat, Chicago, IL 60637 USA. Washington Univ, Sch Med, Dept Psychiat, St Louis, MO 63110 USA. Indiana Univ, Dept Psychiat, Indianapolis, IN 46204 USA. RP Blehar, MC (reprint author), NIMH, Schizophrenia Mood & Other Brain Disorders Progra, DMBDA, Room 18C-14,5600 Fishers Lane, Rockville, MD 20857 USA. OI Nurnberger, John/0000-0002-7674-1767 FU NIMH NIH HHS [MH-46274, MH-46276, MH-46318] NR 21 TC 91 Z9 97 U1 0 U2 4 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPERINTENDENT DOCUMENTS,, WASHINGTON, DC 20402-9325 USA SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1998 VL 34 IS 3 BP 239 EP 243 PG 5 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA 161PH UT WOS:000078297300003 PM 9803748 ER PT J AU Rubinow, DR Schmidt, PJ Roca, CA AF Rubinow, DR Schmidt, PJ Roca, CA TI Hormone measures in reproductive endocrine-related mood disorders: Diagnostic issues SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article DE hormones; premenstrual syndrome; menopause; depression; steroids ID RECEPTOR AB Mood disturbances have been identified in association with changes in levels of reproductive hormones, The use of hormonal measures in the diagnosis of reproductive endocrine-related mood disorders is highly variable, ranging from necessary in perimenopausal depression to irrelevant in premenstrual syndrome, This article describes our view of the use of hormonal measures for diagnostic and research purposes in perimenopausal depression and premenstrual syndrome, We suggest that the understanding of these disorders lies in as yet unidentified contextual factors rather than in hormonal excesses or deficiencies. C1 NIMH, Behav Endocrinol Branch, Bethesda, MD 20892 USA. RP Rubinow, DR (reprint author), NIMH, Behav Endocrinol Branch, Bldg 10,Room 3N238,10 Ctr Dr MSC 1276, Bethesda, MD 20892 USA. NR 10 TC 22 Z9 22 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPERINTENDENT DOCUMENTS,, WASHINGTON, DC 20402-9325 USA SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1998 VL 34 IS 3 BP 289 EP 290 PG 2 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA 161PH UT WOS:000078297300012 PM 9803756 ER PT J AU Schmidt, PJ Roca, CA Rubinow, DR AF Schmidt, PJ Roca, CA Rubinow, DR TI Clinical evaluation in studies of perimenopausal women: Position paper SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article DE perimenopause; mood; depression; estrogen; midlife ID MENOPAUSE; DEPRESSION; SYMPTOMS AB In some women, the perimenopause is associated with the onset of depressive illness, and it is possible that the changes in gonadal steroids accompanying the perimenopause increase an individual's vulnerability to mood destabilization. This article reviews selected aspects of the literature on the relationship between the perimenopause and depression, outlines the evaluation of depression occurring in the context of the perimenopause from a clinical research perspective, and emphasizes methodologic issues to be considered in future studies. C1 NIMH, Behav Endocrinol Branch, Bethesda, MD 20892 USA. RP Schmidt, PJ (reprint author), NIMH, Behav Endocrinol Branch, Bldg 10,Room 3N238,10 Ctr Dr,MSC 1276, Bethesda, MD 20892 USA. NR 19 TC 8 Z9 9 U1 1 U2 1 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPERINTENDENT DOCUMENTS,, WASHINGTON, DC 20402-9325 USA SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1998 VL 34 IS 3 BP 309 EP 311 PG 3 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA 161PH UT WOS:000078297300017 PM 9803761 ER PT J AU Stuart, S O'Hara, MW Blehar, MC AF Stuart, S O'Hara, MW Blehar, MC TI Mental disorders associated with childbearing: Report of the biennial meeting of the Marce Society SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article DE women's health; pregnancy; postpartum; depression; epidemiology ID POSTNATAL DEPRESSION; MAINTENANCE THERAPIES; RECURRENT DEPRESSION; RATIONALE AB This article presents highlights from an International conference focused on mental disorders in association with childbearing. Findings from related research on diagnosis, classification, epidemiology, child outcome, prevention, and treatment are summarized. A need is seen for more research on prevention of childbearing disorders, on the effectiveness of interventions in reducing maternal and child morbidity, on the development of new psychosocial interventions, and on the assessment of the efficacy and safety of somatic treatments in pregnant and lactating women. Mental disorders associated with childbearing are a significant public health problem. Antenatal and postpartum mental disorders are not only associated with morbidity and mortality in affected women, but also with increased morbidity In their children. Despite their prevalence, they are often not recognized by primary care providers, and are hence undertreated. Moreover, physicians are reluctant to treat women with medication during pregnancy and the postpartum for fear of adverse effects on the fetus and the breastfeeding newborn. Little information is available to inform clinicians about the relative risks and benefits of pharmacological treatments. Psychosocial interventions for mental disorders associated with childbearing have not been widely investigated, leaving few options for treatment. The Biennial Meeting of the Marce Society was convened In Iowa City, Iowa, from June 24 to June 28, 1998. The meeting was jointly sponsored by the University of Iowa Department of Psychology, the Colleges of Medicine and Nursing, Postpartum Support International, and by the National Institute of Mental Health, Division of Mental Disorders, Behavioral Research, and AIDS. The Marce Society Is a multi-disciplinary organization that alms to improve the understanding, prevention, and treatment of mental illnesses related to childbearing. International and U.S. participants met to present new research on the epidemiology, Identification, and treatments for childbearing women with mental disorders. Highlights of the meeting are summarized below. C1 Univ Iowa, Dept Psychiat, Iowa City, IA 52242 USA. Univ Iowa, Dept Psychol, Iowa City, IA 52242 USA. NIMH, Div Mental Disorders Behav Res & AIDS, Rockville, MD USA. RP Stuart, S (reprint author), Univ Iowa, Dept Psychiat, 200 Hawkins Dr, Iowa City, IA 52242 USA. NR 19 TC 14 Z9 14 U1 1 U2 4 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPERINTENDENT DOCUMENTS,, WASHINGTON, DC 20402-9325 USA SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1998 VL 34 IS 3 BP 333 EP 338 PG 6 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA 161PH UT WOS:000078297300031 PM 9803766 ER PT J AU Pearsall, R Glick, ID Pickard, D Suppes, T Tauscher, J Jobson, KO AF Pearsall, R Glick, ID Pickard, D Suppes, T Tauscher, J Jobson, KO TI A new algorithm for treating schizophrenia SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article DE schizophrenia; treatment; atypical antipsychotics; comorbidity; extrapyramidal side effects ID DOUBLE-BLIND; RISPERIDONE; HALOPERIDOL; OLANZAPINE; TRIAL; CLOZAPINE; PLACEBO; DISORDERS AB This article presents two algorithms dealing with the management of schizophrenia, One provides a strategy for initiating pharmacologic treatment of schizophrenia and for ongoing medication management. The other covers suggestions for managing several common comorbid psychiatric conditions and some common side effects. The major change from previous algorithms is the suggestion that the newer atypical antipsychotic agents may now be the treatment of choice for initiating therapy in most clinical situations. C1 Univ Tennessee, Sch Med, Int Psychopharmacol Algorithm Project, Schizophrenia Work Grp, Knoxville, TN USA. Yale Univ, New Haven, CT USA. Stanford Univ, Palo Alto, CA 94304 USA. NIMH, Bethesda, MD 20892 USA. SW Texas State Univ, Dallas, TX USA. Univ Vienna, Vienna, Austria. RP Jobson, KO (reprint author), 9405 Pk W Blvd, Knoxville, TN 37923 USA. EM kenjobson@ipap.org RI Tauscher, Johannes/M-5976-2016 NR 26 TC 9 Z9 9 U1 0 U2 1 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPERINTENDENT DOCUMENTS,, WASHINGTON, DC 20402-9325 USA SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1998 VL 34 IS 3 BP 349 EP 353 PG 5 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA 161PH UT WOS:000078297300024 PM 9803768 ER PT J AU Dhokalia, A Parsons, DJ Anderson, DE AF Dhokalia, A Parsons, DJ Anderson, DE TI Resting end-tidal CO2 association with age, gender, and personality SO PSYCHOSOMATIC MEDICINE LA English DT Article DE age; breathing; carbon dioxide; hypertension; neuroticism; personality ID ACID-BASE-EQUILIBRIUM; PRESSURE; HYPOVENTILATION; HYPERCAPNIA; INHIBITION; MICROPIGS AB Objectives: A previous study found that individuals with blood pressure sensitivity to high sodium intake tend to have a high resting partial pressure of end-tidal CO2 (PetCO(2)). The present study analyzed the test-retest reliability of individual PetCO(2) over 6 months, and the association of individual PetCO(2) with age, gender, and personality characteristics. Methods: PetCO(2) of 104 men and women (mean ages 42.1 +/- 1.5 years) was monitored via a respiratory gas monitor for 25 minutes during each of three sessions over an 11-day interval, and 59 subjects also participated in a 25-minute Follow-up session 261 +/- 10 days later. Each subject completed the NEO Personality Inventory. Results: PetCO(2) remained stable within and between monitoring sessions over a 6-month period. PetCO(2) was higher in men than in women, and decreased progressively over the life span. PetCO(2) was not correlated with the Extraversion, Openness, Agreeableness, or Conscientiousness Scales of the NEO Personality Inventory, but was highly positively associated with the Neuroticism Scale of the NEO Personality Inventory, and with its subscales. Conclusions: High resting end-tidal CO2 tends to be a stable individual characteristic that is accompanied by a tendency to worry and experience negative emotions. C1 NIA, Gerontol Res Ctr, Beh Hypertens Sect, Baltimore, MD 21224 USA. RP Anderson, DE (reprint author), NIA, Gerontol Res Ctr, Beh Hypertens Sect, 4940 Eastern Ave, Baltimore, MD 21224 USA. NR 24 TC 22 Z9 22 U1 1 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD JAN-FEB PY 1998 VL 60 IS 1 BP 33 EP 37 PG 5 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA YV306 UT WOS:000071810000006 PM 9492236 ER PT J AU Kaufmann, PG McMahon, RP Becker, LC Bertolet, B Bonsall, R Chaitman, B Cohen, JD Forman, S Goldberg, AD Freedland, K Ketterer, MW Krantz, DS Pepine, CJ Raczynski, J Stone, PH Taylor, H Knatterud, GL Sheps, DS AF Kaufmann, PG McMahon, RP Becker, LC Bertolet, B Bonsall, R Chaitman, B Cohen, JD Forman, S Goldberg, AD Freedland, K Ketterer, MW Krantz, DS Pepine, CJ Raczynski, J Stone, PH Taylor, H Knatterud, GL Sheps, DS CA PIMI Investigators TI The psychophysiological investigations of myocardial ischemia (PIMI) study: Objective, methods, and variability of measures SO PSYCHOSOMATIC MEDICINE LA English DT Article DE coronary heart disease; mental stress; myocardial infarction; cardiovascular reactivity; hemodynamics; psychological tests ID CORONARY-ARTERY DISEASE; BETA-ENDORPHIN LEVELS; TORONTO ALEXITHYMIA SCALE; STABLE ANGINA-PECTORIS; GATED BLOOD POOL; MENTAL STRESS; SILENT ISCHEMIA; PAIN PERCEPTION; HEART-RATE; SEGMENT DEPRESSION AB Objective: This study evaluated physiological, neuroendocrine, and psychological status and functioning of patients with coronary artery disease in order to clarify their role in the expression of symptoms during myocardial ischemia (MI), and to establish repeatability of responses to mental stress. Design and methods of the study are presented. Methods: One hundred ninety-six coronary artery disease patients were examined during physical and mental stress tests in four hospitals. Eligibility criteria included narrowing of at least 50% in the diameter of at least one major coronary artery or verified history of myocardial infarction, and evidence of ischemia on an exercise treadmill test. Psychological, biochemical, and autonomic function data were obtained before, during, and after exposure to mental and exercise stressors during 2 or 3 half-days of testing. Ventricular function was assessed by radionuclide ventriculography, and daily ischemia by ambulatory electrocardiography. Sixty patients returned for a short-term mental stress repeatability study. Twenty-nine individuals presumed to be free of coronary disease were also examined to establish reference values for cardiac responses to mental stress. Results: Study participants were 41 to 80 years of age; 83 (42%) had a history of MI, 6 (3%) of congestive heart failure, and 163 (83%) of chest pain; 170 (87%) were men; and 90 (46%) had ischemia accompanied by angina during exercise treadmill testing. Ischemia during ambulatory monitoring was found in 35 of 90 (39%) patients with and 48 of 106 (45%) patients without angina during exercise-provoked ischemia. Intraobserver variability of ejection fraction changes during bicycle exercise and two mental stress tests (Speech and Stroop) was good (kappa = 1.0, .90, and .76, respectively; percent agreement = 100, 97.5, and 93.8%, respectively). Variability of assessed wall motion abnormalities during bicycle exercise was better (kappa, agreement = 85%) than during Speech or Stroop kappa and .57, percent agreement = 70% and 82.5%, respectively). Conclusions: Study design, quality control data, and baseline characteristics of patients enrolled for a clinical study of symptomatic and asymptomatic myocardial ischemia are described. Lower repeatability of reading wall motion abnormalities during mental stress than during exercise may be due to smaller effects on wall motion and lack of an indicator for peak mental stress. C1 Maryland Med Res Inst, Clin Coordinating Ctr, PIMI, Baltimore, MD 21210 USA. NHLBI, Bethesda, MD 20892 USA. Johns Hopkins Univ, Baltimore, MD USA. Emory Univ, Sch Med, Atlanta, GA USA. St Louis Univ, Hlth Sci Ctr, St Louis, MO 63103 USA. Henry Ford Hosp, Detroit, MI 48202 USA. Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA. Univ Alabama, Birmingham, AL USA. Brigham & Womens Hosp, Boston, MA 02115 USA. Univ N Carolina, Chapel Hill, NC USA. Univ Florida, Gainesville, FL USA. RP Kaufmann, PG (reprint author), Maryland Med Res Inst, Clin Coordinating Ctr, PIMI, 600 Wyndhurst Ave, Baltimore, MD 21210 USA. EM KaufmanP@gwgate.nhlbi.nih.gov RI McMahon, Robert/C-5462-2009; Krantz, David/L-5364-2015 OI Krantz, David/0000-0002-1671-1355 FU NHLBI NIH HHS [HV 18120, HV 18114, HV 18119] NR 63 TC 27 Z9 27 U1 2 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD JAN-FEB PY 1998 VL 60 IS 1 BP 56 EP 63 PG 8 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA YV306 UT WOS:000071810000011 PM 9492241 ER PT J AU Carney, RM McMahon, P Freedland, KE Becker, L Krantz, DS Proschan, MA Raczynski, JM Ketterer, MW Knatterud, GL Light, K Lindholm, L Sheps, DS AF Carney, RM McMahon, P Freedland, KE Becker, L Krantz, DS Proschan, MA Raczynski, JM Ketterer, MW Knatterud, GL Light, K Lindholm, L Sheps, DS CA PIMI Investigators TI Reproducibility of mental stress-induced myocardial ischemia in the psychophysiological investigations of myocardial ischemia (PIMI) SO PSYCHOSOMATIC MEDICINE LA English DT Article; Proceedings Paper CT 16th Annual Meeting of the Society-of-Behavioral-Medicine CY MAR 22-25, 1995 CL SAN DIEGO, CALIFORNIA SP Soc Behav Med DE mental stress; myocardial ischemia; reproducibility ID CORONARY-ARTERY DISEASE; DAILY-LIFE; REACTIVITY; INDUCTION AB Objective: Many patients with coronary artery disease (CAD) develop myocardial ischemia in response to mental stress. This has been documented bath in the natural environment and in the laboratory. However, the reproducibility of laboratory mental stress-induced ischemia has not been investigated, Method: Sixty patients with documented CAD and a positive exercise stress test discontinued cardiac medications and underwent two standardized mental stress tests (a timed Stroop Color-Word test and a public speaking task) in a nuclear cardiology laboratory (Visit 1), and repeated this procedure between 2 and 8 weeks later (Visit 2). Measurements of cardiovascular function and neurohormonal responses were obtained throughout testing, and mood state was assessed before and after testing, Results: Sixty-eight percent of the 56 patients with detailed radionuclide data from both visits had consistent responses (ie, ischemia either present during both sessions or absent during both) to the Stroop task (kappa = .29, p = .03), 61% had consistent responses to the speech task (kappa = .20, p = .12), and 60% had consistent responses when ischemia was considered present if it occurred during either the Stroop test, the speech task, or both, and absent if it did not occur during either task (kappa = .22, p = .07). Hemodynamic and neuroendocrine responses to the tests were moderately reproducible. Conclusions: We conclude that two popular laboratory tests for mental stress-induced myocardial ischemia are modestly reproducible. The relatively low reproducibility is probably influenced by uncertainties in detecting relatively small changes in wall motion, habituation of the patient to repeated exposure Lo psychological stressors, and physiological differences in threshold for ischemia on different days of testing. C1 Washington Univ, Sch Med, St Louis, MO USA. Maryland Med Res Inst, Baltimore, MD USA. Johns Hopkins Univ, Baltimore, MD USA. Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA. NHLBI, Bethesda, MD 20892 USA. Univ Alabama, Birmingham, AL USA. Henry Ford Hosp, Detroit, MI 48202 USA. Univ N Carolina, Chapel Hill, NC USA. Univ Florida, Gainesville, FL USA. RP Carney, RM (reprint author), PIMI, Coordinating Ctr, 600 Wyndhurst Ave, Baltimore, MD 21210 USA. RI Krantz, David/L-5364-2015 OI Krantz, David/0000-0002-1671-1355 FU NHLBI NIH HHS [HV 18114, HV 18119, HV 18120] NR 19 TC 13 Z9 13 U1 0 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD JAN-FEB PY 1998 VL 60 IS 1 BP 64 EP 70 PG 7 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA YV306 UT WOS:000071810000012 PM 9492242 ER PT J AU Lerman, C Main, D Audrain, J Caporaso, NE Bowman, ED Lockshin, B Shields, PG Boyd, NR AF Lerman, C Main, D Audrain, J Caporaso, NE Bowman, ED Lockshin, B Shields, PG Boyd, NR TI Evidence suggesting the role of specific genetic factors in cigarette smoking SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract C1 NCI, Pharmacogenet Sect, Rockville, MD USA. NCI, Human Carcinogenesis Lab, Mol Epidemiol Sect, Bethesda, MD 20892 USA. Fox Chase Canc Ctr, Div Populat Sci, Cheltenham, PA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD JAN-FEB PY 1998 VL 60 IS 1 BP 104 EP 104 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA YV306 UT WOS:000071810000065 ER PT J AU Sheffield, D Sheps, DS Light, KC Freedland, KE Carney, RM Coghlan, CG Cohen, JD Goldberg, AD Pepine, CJ Stone, PH Kaufmann, PG McMahon, RP AF Sheffield, D Sheps, DS Light, KC Freedland, KE Carney, RM Coghlan, CG Cohen, JD Goldberg, AD Pepine, CJ Stone, PH Kaufmann, PG McMahon, RP TI Depressive symptoms and heart rate variability: Results from the psychophysiological investigation of myocardial ischemia (PIMI) study. SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract C1 E Tennessee State Univ, Johnson City, TN 37614 USA. UNC, Chapel Hill, NC USA. Univ Washington, Seattle, WA 98195 USA. Univ Alabama, Tuscaloosa, AL 35487 USA. Henry Ford Hosp, Detroit, MI 48202 USA. Univ Florida, Gainesville, FL 32611 USA. Brigham & Womens Hosp, Boston, MA 02115 USA. NHLBI, Bethesda, MD USA. Maryland Med Res Inst, Baltimore, MD USA. RI McMahon, Robert/C-5462-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD JAN-FEB PY 1998 VL 60 IS 1 BP 109 EP 109 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA YV306 UT WOS:000071810000086 ER PT J AU Ory, M AF Ory, M TI Symposium synopsis: Psychosocial and cultural aspects of the menopausal transition. SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract C1 NIA, SWAN Res Grp, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD JAN-FEB PY 1998 VL 60 IS 1 BP 125 EP 125 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA YV306 UT WOS:000071810000152 ER PT J AU Markley, JL Bax, A Arata, Y Hilbers, CW Kaptein, R Sykes, BD Wright, PE Wuthrich, K AF Markley, JL Bax, A Arata, Y Hilbers, CW Kaptein, R Sykes, BD Wright, PE Wuthrich, K TI Recommendations for the presentation of NMR structures of proteins and nucleic acids - (IUPAC Recommendations 1998) SO PURE AND APPLIED CHEMISTRY LA English DT Article ID MAGNETIC-RESONANCE; COUPLING-CONSTANTS; 3-DIMENSIONAL STRUCTURES; FILE; CRYSTALLOGRAPHY; CONFORMATION; CONSTRAINTS; RING; C-13; H-1 AB The recommendations presented here are designed to support easier com munication of NMR data and NMR structures of proteins and nucleic acids through unified nomenclature and reporting standards. Much of this document pertains to the reporting of data in journal articles; however, in the interest of the future development of structural biology, it is desirable that the bulk of the reported information be stored in computer-accessible form and be freely accessible to the scientific community in standardized formats for data exchange. These recommendations stem from an IUPAC-IUBMB-IUPAB inter-union venture with the direct involvement of ICSU and CODATA. The Task Group has reviewed previous formal recommendations and has extended them in the light of more recent developments in the field of biomolecular NMR spectroscopy. Drafts of the recommendations presented here have been examined critically by more than 50 specialists in the field and have gone through two rounds of extensive modification to incorporate suggestions and criticisms. C1 ETH Honggerberg, Inst Mol Biol & Biophys, CH-8093 Zurich, Switzerland. Univ Wisconsin, Dept Biochem, Madison, WI USA. NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. Water Res Inst, Tsukuba, Ibaraki, Japan. Univ Nijmegen, Biophys Chem Lab, Nijmegen, Netherlands. Univ Utrecht, Dept Chem, NL-3508 TC Utrecht, Netherlands. Univ Alberta, Dept Biochem, Edmonton, AB, Canada. Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA. RP Wuthrich, K (reprint author), ETH Honggerberg, Inst Mol Biol & Biophys, CH-8093 Zurich, Switzerland. NR 66 TC 227 Z9 232 U1 1 U2 23 PU BLACKWELL SCIENCE LTD PI OXFORD PA P O BOX 88, OSNEY MEAD, OXFORD OX2 0NE, OXON, ENGLAND SN 0033-4545 J9 PURE APPL CHEM JI Pure Appl. Chem. PD JAN PY 1998 VL 70 IS 1 BP 117 EP 142 DI 10.1351/pac199870010117 PG 26 WC Chemistry, Multidisciplinary SC Chemistry GA ZR753 UT WOS:000074010300018 ER PT B AU Litt, H Horwitz, B AF Litt, H Horwitz, B BE Carson, RE DaubeWitherspoon, ME Herscovitch, P TI Use of nonlinear kernel analysis to evaluate "badness-of-fit" of the transformation of PET images into stereotactic space: Application to Alzheimer's disease SO QUANTITATIVE FUNCTIONAL BRAIN IMAGING WITH POSITRON EMISSION TOMOGRAPHY LA English DT Proceedings Paper CT 3rd International Conference on Quantification of Brain Function with PET (BRAINPET 97) CY JUN 20-22, 1997 CL NIH, BETHESDA, MD HO NIH C1 NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. RP Litt, H (reprint author), NIA, Neurosci Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA BN 0-12-161340-2 PY 1998 BP 125 EP 131 DI 10.1016/B978-012161340-2/50019-6 PG 7 WC Clinical Neurology; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA BL70N UT WOS:000076399800017 ER PT B AU Ruttimann, UE Rio, D Rawlings, RR Andreason, P Hommer, DW AF Ruttimann, UE Rio, D Rawlings, RR Andreason, P Hommer, DW BE Carson, RE DaubeWitherspoon, ME Herscovitch, P TI PET analysis using a variance stabilizing transform SO QUANTITATIVE FUNCTIONAL BRAIN IMAGING WITH POSITRON EMISSION TOMOGRAPHY LA English DT Proceedings Paper CT 3rd International Conference on Quantification of Brain Function with PET (BRAINPET 97) CY JUN 20-22, 1997 CL NIH, BETHESDA, MD HO NIH C1 NIAAA, Sect Brain Electrophysiol & Imaging, Clin Studies Lab, NIH, Bethesda, MD 20892 USA. RP Ruttimann, UE (reprint author), NIAAA, Sect Brain Electrophysiol & Imaging, Clin Studies Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA BN 0-12-161340-2 PY 1998 BP 217 EP 222 DI 10.1016/B978-012161340-2/50034-2 PG 6 WC Clinical Neurology; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA BL70N UT WOS:000076399800032 ER PT B AU Van Horn, JD Ellmore, TM Holt, JL Esposito, G Berman, KF AF Van Horn, JD Ellmore, TM Holt, JL Esposito, G Berman, KF BE Carson, RE DaubeWitherspoon, ME Herscovitch, P TI Multifiltering signal detection and statistical power in brain activation studies SO QUANTITATIVE FUNCTIONAL BRAIN IMAGING WITH POSITRON EMISSION TOMOGRAPHY LA English DT Proceedings Paper CT 3rd International Conference on Quantification of Brain Function with PET (BRAINPET 97) CY JUN 20-22, 1997 CL NIH, BETHESDA, MD HO NIH C1 NIMH, Unit PET, Clin Brain Disorders Branch, NIH, Bethesda, MD 20892 USA. RP Van Horn, JD (reprint author), NIMH, Unit PET, Clin Brain Disorders Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA BN 0-12-161340-2 PY 1998 BP 237 EP 240 DI 10.1016/B978-012161340-2/50037-8 PG 4 WC Clinical Neurology; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA BL70N UT WOS:000076399800035 ER PT B AU Morris, ED Chefer, SI London, ED AF Morris, ED Chefer, SI London, ED BE Carson, RE DaubeWitherspoon, ME Herscovitch, P TI Limitations of binding potential as a measure of receptor function: A two-point correction for the effects of mass SO QUANTITATIVE FUNCTIONAL BRAIN IMAGING WITH POSITRON EMISSION TOMOGRAPHY LA English DT Proceedings Paper CT 3rd International Conference on Quantification of Brain Function with PET (BRAINPET 97) CY JUN 20-22, 1997 CL NIH, BETHESDA, MD HO NIH C1 NIDA, Brain Imaging Ctr, NIH, Baltimore, MD 21224 USA. RP Morris, ED (reprint author), NIDA, Brain Imaging Ctr, NIH, Baltimore, MD 21224 USA. NR 0 TC 4 Z9 4 U1 0 U2 0 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA BN 0-12-161340-2 PY 1998 BP 407 EP 413 DI 10.1016/B978-012161340-2/50063-9 PG 7 WC Clinical Neurology; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA BL70N UT WOS:000076399800061 ER PT B AU Endres, CJ Carson, RE AF Endres, CJ Carson, RE BE Carson, RE DaubeWitherspoon, ME Herscovitch, P TI Characteristics of neurotransmitter competition studies using constant infusion of tracer SO QUANTITATIVE FUNCTIONAL BRAIN IMAGING WITH POSITRON EMISSION TOMOGRAPHY LA English DT Proceedings Paper CT 3rd International Conference on Quantification of Brain Function with PET (BRAINPET 97) CY JUN 20-22, 1997 CL NIH, BETHESDA, MD HO NIH C1 NIH, Positron Emiss Tomog Dept, Bethesda, MD 20892 USA. RP Endres, CJ (reprint author), NIH, Positron Emiss Tomog Dept, Bethesda, MD 20892 USA. RI Carson, Richard/H-3250-2011 OI Carson, Richard/0000-0002-9338-7966 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA BN 0-12-161340-2 PY 1998 BP 435 EP 439 DI 10.1016/B978-012161340-2/50067-6 PG 5 WC Clinical Neurology; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA BL70N UT WOS:000076399800065 ER PT J AU Leggett, RW Bouville, A Eckerman, KF AF Leggett, RW Bouville, A Eckerman, KF TI Reliability of the ICRP's systemic biokinetic models SO RADIATION PROTECTION DOSIMETRY LA English DT Article; Proceedings Paper CT Workshop on Intakes of Radionuclides - Occupational and Public Exposure CY SEP 15-18, 1997 CL AVIGNON, FRANCE SP British Nuclear Fuels plc, COGEMA, Colenco Power Engn Ltd, Deutsche Gesell Bau & Betrieb Endlagem Abfallstoffe mbH, Electricite de France, Gesell Nukl Serv GmbH, Hemispheres, Plessis Robinson, Nukem Nukl, Siemens, TUV, Hanover, Germany, TUV, Munich, Germany, Vereinig Deutsch Elektrizitatswerke ID HEALTHY-MEN; METABOLISM; PLUTONIUM; DOSIMETRY; EXCRETION; RETENTION; REGISTRY AB The sources, quality, and completeness of data underlying the biokinetic models of the International Commission on Radiological Protection are discussed and the associated uncertainties in those models as dosimetric tools examined. After a general discussion of the relative merits of different sources of biokinetic data, the data base and associated model uncertainties are evaluated for nine environmentally important radionuclides representing various levels of knowledge of biokinetics in humans: H-3, Co-60, Sr-90, Zr-95, Ru-106, Sb-125, Cs-137, Ra-226, and Pu-239. Attention is focused mainly on the behaviour of radionuclides after their absorption to blood. C1 Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA. NCI, Radiat Effects Branch, Bethesda, MD 20892 USA. RP Leggett, RW (reprint author), Oak Ridge Natl Lab, Bldg 1060COM,MS6480, Oak Ridge, TN 37831 USA. NR 37 TC 31 Z9 33 U1 2 U2 8 PU NUCLEAR TECHNOLOGY PUBL PI ASHFORD PA PO BOX 7, ASHFORD, KENT, ENGLAND TN23 1YW SN 0144-8420 J9 RADIAT PROT DOSIM JI Radiat. Prot. Dosim. PY 1998 VL 79 IS 1-4 BP 335 EP 342 PG 8 WC Environmental Sciences; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA 126JA UT WOS:000076289100073 ER PT J AU Qin, J Herring, CJ Zhang, XL AF Qin, J Herring, CJ Zhang, XL TI De novo peptide sequencing in an ion trap mass spectrometer with O-18 labeling SO RAPID COMMUNICATIONS IN MASS SPECTROMETRY LA English DT Article ID DECOMPOSITION AB De novo peptide sequencing in an ion trap mass spectrometer coupled on-line with a capillary HPLC using O-18 labeling provides a viable alternative to the method using the combination of nanospray, O-18 labeling and a quadrupole/time-of-flight mass spectrometer. Seven to sixteen amino acid residues can be sequenced from the liquid chromatography/randem mass spectrometry (LC/MS/MS) spectra. This approach combines the benefit of capillary LC and the high sensitivity of the ion trap operated in the MS/MS mode. The wide availability of the LCQ mass spectrometer makes this approach readily adaptable to the biological mass spectrometry community. (C) 1998 John Wiley & Sons, Ltd. C1 NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Qin, J (reprint author), NHLBI, Biophys Chem Lab, NIH, Bldg 10,Room 7,N307,9000 Rockville Pike, Bethesda, MD 20892 USA. NR 17 TC 58 Z9 58 U1 0 U2 2 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX PO19 1UD, ENGLAND SN 0951-4198 J9 RAPID COMMUN MASS SP JI Rapid Commun. Mass Spectrom. PY 1998 VL 12 IS 5 BP 209 EP 216 DI 10.1002/(SICI)1097-0231(19980314)12:5<209::AID-RCM141>3.0.CO;2-S PG 8 WC Chemistry, Analytical; Spectroscopy SC Chemistry; Spectroscopy GA YZ109 UT WOS:000072221200001 PM 9519474 ER PT J AU DeGnore, JP Konig, S Barrett, WC Chock, PB Fales, HM AF DeGnore, JP Konig, S Barrett, WC Chock, PB Fales, HM TI Identification of the oxidation states of the active site cysteine in a recombinant protein tyrosine phosphatase by electrospray mass spectrometry using on-line desalting SO RAPID COMMUNICATIONS IN MASS SPECTROMETRY LA English DT Article ID DEPHOSPHORYLATION AB The oxidation state of the cysteine residue at the active site of human protein tyrosine phosphatase (PTP-1B) greatly affects its enzymatic activity. We wished to examine peroxide-treated preparations for modifications of this enzyme with electrospray mass spectrometry in order to determine the locations and oxidation states of the cysteines or other residues involved in the process. Since these reaction products contained large amounts of salts and buffers, they required desalting prior to analysis. Existing on- and off-line methods presented certain difficulties in handling and sample usage. Based on recent experience with direct syringe admission of sample, we developed a procedure as a simple, inexpensive alternative to full highperformance liquid chromatography systems that provides on-line desalting using only a few mu L of sample. The method was applied to the analysis of oxidized PTP-1B preparations where conversion of cysteine 215 to both sulfinic and sulfonic acid residues was demonstrated. (C) 1998 John Wiley & Sons, Ltd. C1 NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. NHLBI, Metab Regulat Sect, Biochem Lab, NIH, Bethesda, MD 20892 USA. RP Fales, HM (reprint author), NHLBI, Biophys Chem Lab, NIH, Bldg 10,Room 7N 318,9000 Rockville Pike, Bethesda, MD 20892 USA. RI Konig, Simone/B-6504-2008 OI Konig, Simone/0000-0003-0672-7246 NR 23 TC 14 Z9 14 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX PO19 1UD, ENGLAND SN 0951-4198 J9 RAPID COMMUN MASS SP JI Rapid Commun. Mass Spectrom. PY 1998 VL 12 IS 20 BP 1457 EP 1462 PG 6 WC Chemistry, Analytical; Spectroscopy SC Chemistry; Spectroscopy GA 130DB UT WOS:000076503200006 PM 9796533 ER PT J AU Fail, PA Chapin, RE Price, CJ Heindel, JJ AF Fail, PA Chapin, RE Price, CJ Heindel, JJ TI General, reproductive, developmental, and endocrine toxicity of boronated compounds SO REPRODUCTIVE TOXICOLOGY LA English DT Article; Proceedings Paper CT 1996 Annual Meeting of the Society-for-Industrial-Microbiology CY AUG 04-08, 1996 CL RES TRIANGLE PK, NORTH CAROLINA SP Soc Ind Microbiol DE boric acid; borate; toxicity; reproductive; endocrine; developmental ID BIRTH-RATE ASSESSMENT; BORIC-ACID TOXICITY; TESTICULAR TOXICITY; DRINKING-WATER; MALE EMPLOYEES; MICE; METABOLISM; RECOVERY; EXPOSURE; ANALOGS AB Boric acid and inorganic berates are abundant in nature, They are widely used in industrial, agricultural, cosmetic, and numerous smaller applications, These compounds are toxic to all species tested at high doses, but they are not carcinogenic or mutagenic, The major toxicities are reproductive and developmental, Testicular effects occurred at similar to 26 mg boron equivalents/kg body weight (bw)/d (26 mg boron equivalent (BE)/kg bw/d). New data on endocrine toxicity includes altered follicle stimulating hormone and testosterone within 14 d of treatment. Because these hormonal changes may be secondary effects of testicular toxicity, berates are not suspect as endocrine disrupters, The most sensitive of all the endpoints are prenatal growth and morphologic development in the rat; these changes occurred at a dose of 12.9 mg BE/kg bw/d. The no observed adverse effect level for rat fetal development was 9.6 mg/kg BE, Considering the estimated human exposure levels and a safety factor of 30, humans are not at significant risk of reproductive failure due to berates from environmental sources, The margin of exposure is estimated at 72 for males and 129 for females, Thus, the likelihood of human toxicity caused by boric acid and inorganic berates from exposure during normal activities is remote. C1 Res Triangle Inst, Ctr Life Sci & Toxicol, Dreyfus Lab 141, Res Triangle Pk, NC 27709 USA. NIEHS, Res Triangle Pk, NC 27709 USA. RP Fail, PA (reprint author), Res Triangle Inst, Ctr Life Sci & Toxicol, Dreyfus Lab 141, POB 12194, Res Triangle Pk, NC 27709 USA. OI Chapin, Robert/0000-0002-5997-1261 FU NIEHS NIH HHS [N01-ES-65141, N01-ES-95255] NR 88 TC 66 Z9 81 U1 0 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0890-6238 J9 REPROD TOXICOL JI Reprod. Toxicol. PD JAN-FEB PY 1998 VL 12 IS 1 BP 1 EP 18 DI 10.1016/S0890-6238(97)00095-6 PG 18 WC Reproductive Biology; Toxicology SC Reproductive Biology; Toxicology GA YM715 UT WOS:000071093600001 PM 9431568 ER PT J AU Miller, RK Hendrickx, AG Mills, JL Hummler, H Wiegand, UW AF Miller, RK Hendrickx, AG Mills, JL Hummler, H Wiegand, UW TI Periconceptional vitamin A use: How much is teratogenic? SO REPRODUCTIVE TOXICOLOGY LA English DT Article DE tetratogenicity; retinol; retinyl esters; periconceptional vitamin A use ID NEURAL-TUBE DEFECTS; TRANS-RETINOIC ACID; CYNOMOLGUS MONKEY; BETA-CAROTENE; A INTAKE; LIQUID-CHROMATOGRAPHY; 13-CIS-RETINOIC ACID; HYPERVITAMINOSIS-A; EARLY-PREGNANCY; PLASMA AB The objective of the review is to determine whether preformed vitamin A (retinol and retinyl esters) is teratogenic at dosages commonly used by women living in industrialized countries, Published human and animal data and research developed by the authors are reviewed, It is well known that vitamin A is essential for normal reproduction and development, Although doses of 10,000 IU/d or less of preformed vitamin A (retinyl esters and retinol) are considered safe, doses >10,000 IU/d as supplements have been reported to cause malformations in a single epidemiologic study, Nonhuman primate data show no teratogenicity at doses of 30,000 IU/d. Daily periconceptional exposures greater than 25,000 IU/d of preformed vitamin A have not been sufficiently studied to establish specific risk, Because no study reports adverse effects of 10,000 IU/d preformed vitamin A supplements and this dose is more than the Recommended Dietary Allowance for pregnant women (2670 IU or 800 RE/d), we recommend that women living in industrialized countries or who otherwise have nutritionally adequate diets may not need to ingest more than the Recommended Dietary Allowance of preformed vitamin A as supplements, If periconceptional vitamin A exposures to levels up to 30,000 IU/d (9,000 mu g RE/d) do occur unintentionally, multiple animal studies do support only very low risk, Human epidemiologic studies do not establish at what level vitamin A becomes teratogenic; however, pharmacokinetic data presented in this paper indicate that blood levels of retinoids from women taking 30,000 IU/d of preformed vitamin A are not greater than retinoid blood levels in pregnant women during the first trimester who delivered healthy babies, Interestingly, neither teratogenicity nor vitamin A toxicity has been observed in multiple species exposed to high doses of beta-carotene. (C) 1998 Elsevier Science Inc. C1 Univ Rochester, Med Ctr, Sch Med & Dent, Dept Obstet Gynecol, Rochester, NY 14642 USA. Univ Rochester, Med Ctr, Sch Med & Dent, Dept Environm Med, Rochester, NY 14642 USA. Univ Calif Davis, Calif Reg Primate Res Ctr, Davis, CA USA. NICHHD, Pediat Epidemiol Sect, NIH, Bethesda, MD 20892 USA. F Hoffmann La Roche & Co Ltd, Dept Toxicol, CH-4002 Basel, Switzerland. F Hoffmann La Roche & Co Ltd, Dept Clin Pharmacol, CH-4002 Basel, Switzerland. RP Miller, RK (reprint author), Univ Rochester, Med Ctr, Sch Med & Dent, Dept Obstet Gynecol, 601 Elmwood Ave, Rochester, NY 14642 USA. FU NCRR NIH HHS [RR 00169] NR 89 TC 59 Z9 64 U1 0 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0890-6238 J9 REPROD TOXICOL JI Reprod. Toxicol. PD JAN-FEB PY 1998 VL 12 IS 1 BP 75 EP 88 DI 10.1016/S0890-6238(97)00102-0 PG 14 WC Reproductive Biology; Toxicology SC Reproductive Biology; Toxicology GA YM715 UT WOS:000071093600008 PM 9431575 ER PT J AU Flower, RW Csaky, KG Murphy, RP AF Flower, RW Csaky, KG Murphy, RP TI Disparity between fundus camera and scanning laser ophthalmoscope indocyanine green imaging of retinal pigment epithelium detachments SO RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES LA English DT Article DE detachment; dye; indocyanine green; leakage; retinal pigment epithelium ID CHOROIDAL NEOVASCULARIZATION; VIDEOANGIOGRAPHY AB Purpose: Indocyanine green (ICG) angiograms of each of five patients with retinal pigment epithelium (RPE) detachments were made using first a Topcon fundus camera and then a Heidelberg scanning laser ophthalmoscope (SLO); for each patient, both types of angiograms were obtained on the same day. In each case, the serous fluid appeared bright throughout the fundus camera studies and dark throughout the SLO studies. This study sought to explain the disparity in the appearance of the lesions in the two kinds of images and to determine whether there was dye in the serous fluid. Methods: Simple model eyes were constructed to demonstrate the effects of Mie light scatter and integrating sphere behavior of the sclera on ICG image formation by the fundus camera and SLO optics. Analysis was made of both the clinical angiograms and model eye images to structure an explanation for the disparate RPE detachment angiographic images. Results: Indocyanine green fluorescent light from choroidal vessels adjacent to the lesions and scattered by the turbid serous fluid accounted for the lesion brightness seen in the fundus camera images. The models confirmed that SLOs suppress scattered light. Conclusions: The apparent fluorescence of serous fluid beneath RPE detachments in fundus camera early-phase ICG angiogram images is not attributable to the presence of dye; rather, it appears to be attributable to serous fluid light scatter of fluorescent light arising from adjacent fluorescent structures. This light scatter is a consequence of the fundus camera illumination and recording optics and is not present in SLO-generated images. The necessity of understanding such phenomena as absorption, diffraction, polarization, and scatter of light and routinely applying them to ICG angiogram interpretation is underscored when it is shown that they offer simple explanations for unusual or unexpected angiographic results, as in the case of the patients with RPE detachment discussed here. C1 Retina Inst Maryland, Towson, MD USA. NEI, Bethesda, MD 20892 USA. Univ Maryland, Baltimore, MD 21201 USA. RP Flower, RW (reprint author), Retina Inst Maryland, 4 Reservoir Circle,2nd Floor, Baltimore, MD 21208 USA. NR 10 TC 31 Z9 32 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0275-004X J9 RETINA-J RET VIT DIS JI Retin.-J. Retin. Vitr. Dis. PY 1998 VL 18 IS 3 BP 260 EP 268 PG 9 WC Ophthalmology SC Ophthalmology GA ZU331 UT WOS:000074186000012 PM 9654419 ER PT J AU Lee, PP Meredith, LS Whitcup, SM Spritzer, K Hays, RD AF Lee, PP Meredith, LS Whitcup, SM Spritzer, K Hays, RD TI A comparison of self-reported utilization of ophthalmic care for diabetes in managed care versus fee-for-service SO RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES LA English DT Article DE diabetes screening; managed care; preventive health ID PREPAID AB Purpose: To assess the association between structural factors in the health care delivery system and self-reported utilization of ophthalmic services by patients with diabetes in the Medical Outcomes Study (MOS), Methods: Self-reported utilization of ophthalmic services within the 6 months preceding enrollment into the MOS among 522 of 567 individuals with diabetes in the MOS longitudinal panel was measured. Use of eye care services was regressed (logistic model) on patient demographics, geographic location, physician specialty, type of practice, and finance plan (prepaid or fee-for-service). Results: None of the variables was significantly associated with a higher or lower likelihood of having used ophthalmic services in the preceding 6 months. Thus, no difference between prepaid or fee-for-service plans or among solo practice, large multispecialty groups, or HMOs were identified. Having seen an internist, family practitioner, or diabetes specialist for diabetes care was not related to use of ophthalmic services, Conclusions: Despite a presumed greater interest in preventive health, prepaid health plans were no more or less likely than the fee-for-service sector to have patients with diabetes reporting an eye examination within the prior 6 months. Thus, steps to improve the rate of eye examinations of diabetics may need to focus beyond the structural elements of the health care delivery system. C1 Rand Corp, Hlth Sci Program, Santa Monica, CA 90407 USA. Univ So Calif, Sch Med, Doheny Eye Inst, Los Angeles, CA USA. NEI, Clin Branch, Bethesda, MD 20892 USA. Univ Calif Los Angeles, Sch Med, Los Angeles, CA 90024 USA. RP Lee, PP (reprint author), Rand Corp, Hlth Sci Program, Santa Monica, CA 90407 USA. RI Hays, Ronald/D-5629-2013; OI Lee, Paul/0000-0002-3338-136X NR 13 TC 7 Z9 7 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 USA SN 0275-004X J9 RETINA-J RET VIT DIS JI Retin.-J. Retin. Vitr. Dis. PY 1998 VL 18 IS 4 BP 356 EP 359 PG 4 WC Ophthalmology SC Ophthalmology GA 110HG UT WOS:000075374900011 PM 9730180 ER PT S AU Hibbeln, JR Salem, N AF Hibbeln, JR Salem, N BE Simopoulos, AP TI Fish consumption may predict a lower prevalence of major depression: A cross-national analysis SO RETURN OF OMEGA-3 FATTY ACIDS INTO THE FOOD SUPPLY: I. LAND-BASED ANIMAL FOOD PRODUCTS AND THEIR HEALTH EFFECTS SE WORLD REVIEW OF NUTRITION AND DIETETICS LA English DT Meeting Abstract CT International Conference on the Return of Omega-3 Fatty Acids into the Food Supply - I Land-Based Animal Food Products and Their Health Effects CY SEP 18-19, 1997 CL NIH, NATCHER CONF CTR, BETHESDA, MD SP Ctr Genet Nutr & Hlth, NIH, Natl Inst Alcohol Abuse & Alcoholism, NICHHD, Designer Egg Producers Assoc Int, ENRECO Inc, F Hoffmann La Roche AG, Flax Council Canada, Martek Biosci Corp, OmegaTech Inc, Pilgrims Pride Eggs Plus, Roche Vitamins Inc, Iams Co, Nutrasweet Kelco Co HO NIH, NATCHER CONF CTR C1 NIAAA, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU KARGER PI BASEL PA POSTFACH, CH-4009 BASEL, SWITZERLAND SN 0084-2230 BN 3-8055-6694-8 J9 WORLD REV NUTR DIET JI World Rev.Nutr.Diet. PY 1998 VL 83 BP 226 EP 226 PG 1 WC Agriculture, Dairy & Animal Science; Nutrition & Dietetics SC Agriculture; Nutrition & Dietetics GA BL12J UT WOS:000074373200024 ER PT J AU Granholm, AC Albeck, D Backman, C Curtis, M Ebendal, T Friden, P Henry, M Hoffer, B Kordower, J Rose, GM Soderstrom, S Bartus, RT AF Granholm, AC Albeck, D Backman, C Curtis, M Ebendal, T Friden, P Henry, M Hoffer, B Kordower, J Rose, GM Soderstrom, S Bartus, RT TI A non-invasive system for delivering neural growth factors across the blood-brain barrier: A review SO REVIEWS IN THE NEUROSCIENCES LA English DT Review DE blood-brain barrier; forebrain septal nuclei; acetylcholine; cholinergic; transplantation; neurotrophins; transferrin receptors; drug delivery; macromolecules ID ANTITRANSFERRIN RECEPTOR ANTIBODY; CENTRAL-NERVOUS-SYSTEM; PEPTIDE DRUG-DELIVERY; FOREBRAIN CHOLINERGIC NEURONS; VECTOR-MEDIATED DELIVERY; RAT BASAL FOREBRAIN; ALZHEIMERS-DISEASE; TRANSFERRIN RECEPTOR; NUCLEUS BASALIS; FACTOR NGF AB Intraventricular administration of nerve growth factor (NGF) in rats has been shown to reduce age-related atrophy of central cholinergic neurons and the accompanying memory impairment, as well as protect these neurons against a variety of perturbations. Since neurotrophins do not pass the blood-brain barrier (BBB) in significant amounts, a non-invasive delivery system for this group of therapeutic molecules needs to be developed, We have utilized a carrier system, consisting of NGF covalently linked to an anti-transferrin receptor antibody (OX-26), to transport biologically active NGF across the BBB, The biological activity of this carrier system was tested using in vitro bioassays and intraocular transplants; we were able to demonstrate that cholinergic markers in both developing and aged intraocular septal grafts were enhanced by intravenous delivery of the OX-26-NGF conjugate, In subsequent experiments,aged (24 months old) Fischer 344 rats received intravenous injections of the OX-26-NGF conjugate for 6 weeks, resulting ina significant improvement in spatial learning in previously impaired rats,but disrupting the learning ability of previously unimpaired rats. Neuroanatomical analyses showed that OX-26-NGF conjugate treatment resulted in a significant increase in cholinergic cell size as well as an upregulation of both low and high affinity NGF receptors in the medial septal region of rats initially impaired in spatial learning. Finally, OX-26-NGF was able to protect striatal cholinergic neurons against excitotoxicity and basal forebrain cholinergic neurons from degeneration associated with chemically-induced loss of target neurons, These results indicate the potential utility of the transferrin receptor antibody delivery system for treatment of neurodegenerative disorders with neurotrophic substances. C1 Univ Colorado, Hlth Sci Ctr, Dept Basic Sci, Denver, CO 80262 USA. Univ Colorado, Hlth Sci Ctr, Dept Pharmacol, Denver, CO 80262 USA. Univ Colorado, Hlth Sci Ctr, Neurosci Program, Denver, CO 80262 USA. Uppsala Univ, Dept Dev Biol, Uppsala, Sweden. Alkermes Inc, Cambridge, MA USA. Periodontix Inc, Watertown, MA USA. NIDA, Intramural Res Program, Baltimore, MD USA. Rush Presbyterian St Lukes Med Ctr, Dept Neurol Sci, Chicago, IL 60612 USA. RI backman, cristina/C-1276-2013 FU NIA NIH HHS [R01 AG010755, AG12122, AG04418]; NIMH NIH HHS [MH49661] NR 154 TC 45 Z9 49 U1 2 U2 3 PU FREUND & PETTMAN PUBLISHERS PI EAST YORKSHIRE PA ENHOLMES HALL, PATRINGTON, EAST YORKSHIRE HU12 OPR, ENGLAND SN 0334-1763 J9 REV NEUROSCIENCE JI Rev. Neurosci. PY 1998 VL 9 IS 1 BP 31 EP 55 PG 25 WC Neurosciences SC Neurosciences & Neurology GA ZT969 UT WOS:000074147200003 PM 9683326 ER PT J AU Illa, I Dalakas, MC AF Illa, I Dalakas, MC TI Dermatomyositis, polymyositis and inclusion body myositis: current concepts SO REVUE NEUROLOGIQUE LA English DT Editorial Material ID CELL RECEPTOR REPERTOIRE; INFLAMMATORY MYOPATHIES; T-CELLS; MUSCLE; EXPRESSION; LYMPHOCYTES AB Dermatomysitis (DM), Polymyosistis (PM), and Inclusion-Body Myositis (IBM) are the three major inflammatory myopathies (IM) (Dalakas, 1991; Karpati et Carpenter, 1993; Engel et al,, 1994; Hohlfeld et Engel, 1994). We will provide an update on the clinical characteristics, diagnostic criteria, and pathogenetic mechanisms that separate each one of these myopathies and discuss the current approach to treatment. C1 Hosp Santa Cruz & San Pablo, Dept Neurol, Neuromuscular Dis Sect, E-08025 Barcelona, Spain. NINDS, Neuromusc Dis Sect, Bethesda, MD 20892 USA. RP Illa, I (reprint author), Hosp Santa Cruz & San Pablo, Dept Neurol, Neuromuscular Dis Sect, Avda SAM Claret 167, E-08025 Barcelona, Spain. NR 33 TC 6 Z9 6 U1 0 U2 1 PU MASSON EDITEUR PI PARIS 06 PA 120 BLVD SAINT-GERMAIN, 75280 PARIS 06, FRANCE SN 0035-3787 J9 REV NEUROL JI Rev. Neurol. PD JAN PY 1998 VL 154 IS 1 BP 13 EP 16 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA YW899 UT WOS:000071985900002 PM 9773019 ER PT B AU Dionne, RA AF Dionne, RA BE Rainsford, KD Powanda, MC TI Evaluation of analgesic mechanisms and NSAIDs for acute pain using the oral surgery model SO SAFETY AND EFFICACY OF NON-PRESCRIPTION (OTC) ANALGESICS AND NSAIDS LA English DT Proceedings Paper CT International Conference on Safety and Efficacy of Non-Prescription (OTC) Analgesics and NSAIDs CY MAR 17, 1997 CL SAN FRANCISCO, CA SP Boots Hlethcare Int, Whitehall Robins ID POSTOPERATIVE PAIN; RHEUMATOID-ARTHRITIS; DENTAL PAIN; IBUPROFEN; SUPPRESSION; FLURBIPROFEN; CODEINE; PLACEBO C1 NIDR, Pain & Neurosensory Mechanisms Branch, NIH, Bethesda, MD 20892 USA. RP Dionne, RA (reprint author), NIDR, Pain & Neurosensory Mechanisms Branch, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. NR 33 TC 2 Z9 3 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS BN 0-7923-8737-6 PY 1998 BP 105 EP 117 PG 13 WC Medicine, General & Internal; Pharmacology & Pharmacy SC General & Internal Medicine; Pharmacology & Pharmacy GA BN28D UT WOS:000081416000009 ER PT S AU Brown, P AF Brown, P BE Brown, F Griffiths, E Horaud, F Petricciani, JC TI Transmission of spongiform encephalopathy through biological products SO SAFETY OF BIOLOGICAL PRODUCTS PREPARED FROM MAMMALIAN CELL CULTURE SE DEVELOPMENTS IN BIOLOGICAL STANDARDIZATION LA English DT Article; Proceedings Paper CT WHO Consultation Conference on the Safety of Biological Products Prepared from Mammalian Cell Substrates CY SEP 29-OCT 01, 1996 CL ANNECY LE VIEUX, FRANCE SP Marcel-Meriuex Fdn, Int Assoc Biol Standardizat (IABS), WHO DE transmissible spongiform encephalopathy; transmissible cerebral amyloidosis; kuru; Creutzfeldt-Jakob disease; prion disease ID CREUTZFELDT-JAKOB-DISEASE; PERSON TRANSMISSION; HUMAN-BLOOD; DURA MATER; TISSUE AB The transmissible spongiform encephalopathies (TSE) are rare but fatal neurological diseases that exist in sporadic, familial, and environmentally acquired forms. Environmentally acquired disease has until now been mostly iatrogenic in origin, recognized as responsible for nearly 200 deaths during the past 20 years. The two most important causes have been contamination of cadaver-derived human growth hormone and dura mater grafts, but a few instances related to contaminated neurosurgical instruments and corneal grafts have also occurred. Currently, a great deal of concern is being voiced about the potential risk of acquiring disease through blood or blood products, and although there is no supporting epidemiological evidence for such a danger, experiments are underway to define which blood components or plasma derivatives might pose the greatest problem. C1 NINDS, Cent Nervous Syst Studies Lab, NIH, Bethesda, MD 20892 USA. RP Brown, P (reprint author), NINDS, Cent Nervous Syst Studies Lab, NIH, Bldg 36,Room 5B21, Bethesda, MD 20892 USA. NR 16 TC 18 Z9 18 U1 1 U2 1 PU KARGER PI BASEL PA POSTFACH, CH-4009 BASEL, SWITZERLAND SN 0301-5149 BN 3-8055-6732-4 J9 DEV BIOL STAND JI Dev.Biol.Stand. PY 1998 VL 93 BP 73 EP 78 PG 6 WC Biology; Biotechnology & Applied Microbiology; Cell Biology; Pharmacology & Pharmacy SC Life Sciences & Biomedicine - Other Topics; Biotechnology & Applied Microbiology; Cell Biology; Pharmacology & Pharmacy GA BL51Y UT WOS:000075761500010 PM 9737380 ER PT S AU Piatigorsky, J AF Piatigorsky, J BE Kukuruzinska, MA Tabak, LA TI Multifunctional lens crystallins and corneal enzymes - More than meets the eye SO SALIVARY GLAND BIOGENESIS AND FUNCTION SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT Conference on Salivary Gland Biogenesis and Function CY NOV 07-10, 1996 CL ARLIE, VA SP NIDR ID HEAT-SHOCK PROTEIN; ALDEHYDE DEHYDROGENASE; ALPHA-CRYSTALLIN; SOLUBLE-PROTEINS; TRANSGENIC MICE; GENE-REGULATION; BOVINE CORNEA; RECRUITMENT; EVOLUTION; CHICKEN AB The abundant water-soluble proteins, called crystallins, of the transparent, refractive eye lens have been recruited from metabolic enzymes and stress-protective proteins by a process called "gene sharing." Many crystallins are also present at lower concentration in nonocular tissues where they have nonrefractive roles. The complex expression pattern of the mouse alpha B-crystallin/small heat shock protein gene is developmentally controlled at the transcriptional level by a combinatorial use of shared and lens-specific regulatory elements. A number of crystallin genes, including that for alpha B-crystallin, are activated by Pax-6, a conserved transcription factor for eye evolution. Aldehyde dehydrogenase class 3 and transketolase are metabolic enzymes comprising extremely high proportions of the water-soluble proteins of the cornea and may have structural as well as enzymatic roles, reminiscent of lens enzyme-crystallins. inductive processes appear to be important for the corneal-preferred expression of these enzymes. The use of the same protein for entirely different functions by a gene-sharing mechanism may be a general strategy based on evolutionary tinkering at the level of gene regulation. C1 NEI, Mol & Dev Biol Lab, NIH, Bethesda, MD 20892 USA. RP NEI, Mol & Dev Biol Lab, NIH, Bldg 6,Room 201, Bethesda, MD 20892 USA. EM joram@helix.nih.gov NR 46 TC 95 Z9 96 U1 0 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-136-7; 1-57331-135-9 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 842 BP 7 EP 15 DI 10.1111/j.1749-6632.1998.tb09626.x PG 9 WC Dentistry, Oral Surgery & Medicine; Substance Abuse; Multidisciplinary Sciences SC Dentistry, Oral Surgery & Medicine; Substance Abuse; Science & Technology - Other Topics GA BL11Q UT WOS:000074330900003 PM 9599288 ER PT S AU Slavkin, H AF Slavkin, H BE Kukuruzinska, MA Tabak, LA TI Preparing for the twenty-first century SO SALIVARY GLAND BIOGENESIS AND FUNCTION SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Salivary Gland Biogenesis and Function CY NOV 07-10, 1996 CL ARLIE, VIRGINIA SP NIDR C1 NIDR, NIH, Bethesda, MD 20892 USA. RP Slavkin, H (reprint author), NIDR, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-135-9 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1998 VL 842 BP XI EP XI DI 10.1111/j.1749-6632.1998.tb09624.x PG 1 WC Dentistry, Oral Surgery & Medicine; Substance Abuse; Multidisciplinary Sciences SC Dentistry, Oral Surgery & Medicine; Substance Abuse; Science & Technology - Other Topics GA BL11Q UT WOS:000074330900001 ER EF