FN Thomson Reuters Web of Science™ VR 1.0 PT J AU VAUPEL, DB KIMES, AS LONDON, ED AF VAUPEL, DB KIMES, AS LONDON, ED TI COMPARISON OF 7-NITROINDAZOLE WITH OTHER NITRIC-OXIDE SYNTHASE INHIBITORS AS ATTENUATORS OF OPIOID WITHDRAWAL SO PSYCHOPHARMACOLOGY LA English DT Article DE NITRIC OXIDE SYNTHASE; MORPHINE; DEPENDENCE; OPIOID WITHDRAWAL; 7-NITROINDAZOLE; L-IMINOETHYL ORNITHINE; L-N-G-NITROARGININE; NITRIC OXIDE; RATS; BLOOD PRESSURE ID MEDIATED HYPERTENSIVE RESPONSE; RECEPTOR ANTAGONIST MK-801; NORMOTENSIVE RATS; BLOOD-PRESSURE; DEPENDENT RATS; MORPHINE; BUPRENORPHINE; CLONIDINE; ACTIVATION; NEURONS AB Previously, we demonstrated that two nonselective inhibitors of nitric oxide synthase (NOS), L-N-G-nitroarginine (L-NNA) and L-N-G-nitroarginine methyl ester (L-NAME), reduced some signs of morphine withdrawal in rats. The present work extended these studies to include 7-nitroindazole (7-NI), an inhibitor specific for cerebral NOS, and N(5)-(1-iminoethyl)-L-ornithine (L-NIO), a potent inhibitor of endothelial NOS. Behavioral effects of these four NOS inhibitors and clonidine, an alpha(2)-adrenoceptor, agonist, on morphine withdrawal in rats were assessed. Rats received one 75-mg morphine pellet subcutaneously (SC). Three days later, NOS inhibitors were administered IP 1 h before withdrawal was precipitated with naloxone (0.5 mg/kg, SC) and scored. 7-NI, L-NIO, L-NAME and L-NNA produced dose-related decreases in weight loss, diarrhea, wet dog shakes and grooming. 7-NI also reduced mastication, salivation and genital effects. Clonidine produced effects similar to 7-NI. In awake, morphine-naive and morphine-dependent rats not subjected to withdrawal, 7-NI was the only NOS inhibitor that did not increase blood pressure. Because 7-NI attenuated more signs of opioid withdrawal than L-NNA, L-NAME or L-NIO without causing hypertension, 7-NI appears to warrant further testing as a potential candidate for human use. C1 UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,BALTIMORE,MD 21201. JOHNS HOPKINS MED INST,DEPT RADIOL,BALTIMORE,MD 21205. RP VAUPEL, DB (reprint author), NIDA,INTRAMURAL RES PROGRAM,NEUROIMAGING & DRUG ACT SECT,BALTIMORE,MD 21224, USA. NR 25 TC 60 Z9 62 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD APR PY 1995 VL 118 IS 4 BP 361 EP 368 PG 8 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA QY586 UT WOS:A1995QY58600001 PM 7568621 ER PT J AU RESNICK, SM COSTA, PT AF RESNICK, SM COSTA, PT TI COMMENTS ON USE OF H-1 MR SPECTROSCOPY FOR DIAGNOSIS OF PROBABLE ALZHEIMER-DISEASE SO RADIOLOGY LA English DT Editorial Material DE AGING; BRAIN, METABOLISM; DEMENTIA; EDITORIALS; MAGNETIC RESONANCE (MR), SPECTROSCOPY RP RESNICK, SM (reprint author), NIA,GERONTOL RES CTR,PERSONAL & COGNIT LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 9 TC 6 Z9 6 U1 0 U2 0 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD APR PY 1995 VL 195 IS 1 BP 14 EP 15 PG 2 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA QM663 UT WOS:A1995QM66300005 PM 7892455 ER PT J AU DAVIDSON, AJ CHOYKE, PL HARTMAN, DS DAVIS, CJ AF DAVIDSON, AJ CHOYKE, PL HARTMAN, DS DAVIS, CJ TI RENAL MEDULLARY CARCINOMA-ASSOCIATED WITH SICKLE-CELL TRAIT - RADIOLOGIC FINDINGS SO RADIOLOGY LA English DT Article DE KIDNEY NEOPLASMS, DIAGNOSIS; SICKLE CELL TRAIT ID COMPUTED-TOMOGRAPHY; ULTRASOUND; LYMPHOMA; KIDNEY AB PURPOSE: To correlate the radiologic and pathologic findings in patients with renal medullary carcinoma and sickle cell trait. MATERIALS AND METHODS: Radiologic studies of five pathologically proved cases of renal medullary carcinoma were retrospectively correlated with gross pathologic findings. Excretory urograms, computed tomographic (CT) scans, sonograms, photographs of the gross surgical specimens, and an angiogram were available for review. Each case was analyzed for tumor location, pattern of growth, contrast enhancement and echotexture, angiographic pattern, and stage. RESULTS: All tumors arose centrally within the kidney, grew in an infiltrative pattern, and invaded the renal sinus. Caliectasis without pelviectasis was present in three cases. Contrast enhancement and echotexture were heterogeneous in all patients. Tumor necrosis with communication into the collecting system occurred in one patient. The one available angiogram demonstrated hypovascularity. CONCLUSION: Patients with renal medullary carcinoma share particular demographic, clinical, and radiologic features that might enable radiologists to suggest a specific diagnosis. C1 ARMED FORCES INST PATHOL,DEPT GENITOURINARY PATHOL,WASHINGTON,DC 20306. NIH,CTR CLIN,DEPT RADIOL,BETHESDA,MD 20892. PENN STATE UNIV,SCH MED,DEPT RADIOL,HERSHEY,PA. RP DAVIDSON, AJ (reprint author), ARMED FORCES INST PATHOL,AMER REGISTRY PATHOL,DEPT RADIOL PATHOL,14TH & ALASKA AVE NW,WASHINGTON,DC 20306, USA. NR 18 TC 59 Z9 61 U1 0 U2 1 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD APR PY 1995 VL 195 IS 1 BP 83 EP 85 PG 3 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA QM663 UT WOS:A1995QM66300016 PM 7892499 ER PT J AU COSMIDES, GJ AF COSMIDES, GJ TI ELECTRONIC SURVEILLANCE OF THE PHARMACOLOGY-TOXICOLOGY LITERATURE - THE NEED FOR CONTROLLED VOCABULARIES AND REGISTRY SYSTEMS SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Article ID CLASSIFICATION AB Controlled vocabularies of ''preferred names'' and registry systems are essential in electronic indexing, storing, searching, and retrieving the world's published literature. The most efficient and comprehensive search is accomplished by using the preferred name. Without a controlled vocabulary or a registry system, it would be necessary to remember every name that might have been used by authors since January 1966, in order to retrieve all the citations on a chemical from over 7.8 million citations currently in the National Library of Medicine's MEDLINE and its backfiles. The task of creating the list of subject descriptors that make possible the surveillance of published literature via electronic databases requires the participation of the scientific community in developing domain-specific nomenclature, drug classification, controlled vocabularies, and registry systems as well. The biological unions of the International Council of Scientific Unions and its Committee on Data for Science and Technology are major contributors to the establishment and dissemination of standards for biological terminology and nomenclature. The objectives of the IUPHAR Nomenclature Committee include the development of a rational framework for the nomenclature of receptor classes or families and a classification for therapeutic agents. This will help define rules for the characterization and classification of receptors that are stable and easy to comprehend. The International Union of Pharmacology publishes guidelines for the classification of drugs and the nomenclature of receptors and ion channels. (C) 1995 Academic Press, Inc. RP COSMIDES, GJ (reprint author), NATL LIB MED,BETHESDA,MD 20894, USA. RI Henrich, Joseph/A-2403-2009 NR 11 TC 1 Z9 1 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0273-2300 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD APR PY 1995 VL 21 IS 2 BP 208 EP 210 DI 10.1006/rtph.1995.1029 PG 3 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA QY863 UT WOS:A1995QY86300002 PM 7644707 ER PT J AU BOORMAN, GA SEELY, JC AF BOORMAN, GA SEELY, JC TI THE LACK OF AN OVARIAN EFFECT OF LIFETIME TALC EXPOSURE IN F344/N RATS AND B6C3F1 MICE SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Article; Proceedings Paper CT International-Society-of-Regulatory-Toxicology-and-Pharmacology/US-FDA Workshop on Talc - Consumer Uses and Health Perspectives CY JAN 31-FEB 01, 1994 CL NIH, BETHESDA, MD SP NIH HO NIH C1 NIEHS,PATHCO INC,RES TRIANGLE PK,NC 27709. RP BOORMAN, GA (reprint author), NIEHS,ENVIRONM TOXICOL PROGRAM,PATHOL BRANCH,RES TRIANGLE PK,NC 27709, USA. NR 5 TC 6 Z9 6 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0273-2300 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD APR PY 1995 VL 21 IS 2 BP 242 EP 243 DI 10.1006/rtph.1995.1035 PG 2 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA QY863 UT WOS:A1995QY86300008 PM 7644712 ER PT J AU HARLOW, BL HARTGE, PA AF HARLOW, BL HARTGE, PA TI A REVIEW OF PERINEAL TALC EXPOSURE AND RISK OF OVARIAN-CANCER SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Article; Proceedings Paper CT International-Society-of-Regulatory-Toxicology-and-Pharmacology/US-FDA Workshop on Talc - Consumer Uses and Health Perspectives CY JAN 31-FEB 01, 1994 CL NIH, BETHESDA, MD SP NIH HO NIH ID FOLLOW-UP; MORTALITY; WORKERS; TUMORS; WOMEN AB The authors provide a detailed review of the events that led to the interest in talc as a possible ovarian carcinogen, the epidemiological studies published to date, and their perspective on the interpretation of the findings including potential limitations, biases, and issues surrounding the plausibility of a causal association. The authors conclude that the range of relative risk estimates from epidemiology, 1.0 to 1.8, is plausible, but that additional epidemiologic studies, especially prospective investigations are needed, In addition, clinicopathological studies are needed to confirm or deny the reports of talc embedded in human ovarian tissue and reports of talc migration through the human female reproductive tract. (C) 1995 Academic Press, Inc. C1 NCI,DIV CANC ETIOL,BETHESDA,MD 20205. RP HARLOW, BL (reprint author), HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,CTR OBSTET & GYNECOL EPIDEMIOL,BOSTON,MA 02115, USA. NR 35 TC 26 Z9 26 U1 2 U2 3 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0273-2300 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD APR PY 1995 VL 21 IS 2 BP 254 EP 260 DI 10.1006/rtph.1995.1039 PG 7 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA QY863 UT WOS:A1995QY86300012 PM 7644715 ER PT J AU BENFIELD, TL VANSTEENWIJK, R NIELSEN, TL DICHTER, JR LIPSCHIK, GY JENSEN, BN JUNGE, J SHELHAMER, JH LUNDGREN, JD AF BENFIELD, TL VANSTEENWIJK, R NIELSEN, TL DICHTER, JR LIPSCHIK, GY JENSEN, BN JUNGE, J SHELHAMER, JH LUNDGREN, JD TI INTERLEUKIN-8 AND EICOSANOID PRODUCTION IN THE LUNG DURING MODERATE TO SEVERE PNEUMOCYSTIS-CARINII PNEUMONIA IN AIDS - A ROLE OF INTERLEUKIN-8 IN THE PATHOGENESIS OF PNEUMOCYSTIS-CARINII PNEUMONIA SO RESPIRATORY MEDICINE LA English DT Article ID ACQUIRED-IMMUNODEFICIENCY-SYNDROME; CONTROLLED TRIAL; VIRUS INFECTION; CELL-LINE; CORTICOSTEROIDS; CYTOKINE; THERAPY AB Pneumocystis carinii pneumonia (PCP) may cause severe respiratory distress. This is believed to be partly caused by the accumulation of neutrophils in the lung. Interleukin-8 (IL-8) and leukotriene B-4 (LTB(4)) are potent neutrophil chemo-attractants and activators. Eicosanoids [i.e. prostaglandins (PG) and leukotrienes (LT)] are pro-inflammatory mediators released from arachidonic acid by action of phospholipase A(2) (PLA(2)) and have been implicated in the host response to micro-organisms, Bronchoalveolar lavage (BAL) was performed on patients with PCP as part of a randomized study of adjuvant corticosteroids vs. placebo, in addition to standard antimicrobial therapy. Re-bronchoscopy was offered at day 10. BAL fluid was available for 26 patients who had follow-up bronchoscopy performed. At diagnosis, IL-8 levels were elevated in patients with PCP, compared to healthy controls, and correlated with relative BAL neutrophilia and P(A-a)O-2. LTB(4) was also elevated in PCP, but failed to correlate with either BAL neutrophilia or P(A-a)O-2. PLA, activity in patients correlated with IL-8 levels and BAL neutrophilia, but not with P(A-a)O-2. A trend towards a decrease in IL-8 levels in BAL fluid was detected in the corticosteroid-treated patients from days 0-10, whereas no change was detected in the placebo group. No change in levels of LTB(4), LTC(4), PGE(2), PGF(2 alpha), and PLA(2) were detected over time in either treatment group. This study establishes a correlation between IL-8, BAL neutrophilia and P(A-a)O-2, and suggests a role of IL-8 as a mediator in the pathogenesis of PCP, whereas the role of eicosanoids seems less clear. C1 UNIV COPENHAGEN,HVIDOVRE HOSP,DEPT PATHOL,DK-2650 HVIDOVRE,DENMARK. UNIV COPENHAGEN,RIGSHOSP,DEPT INFECT DIS,DK-2650 HVIDOVRE,DENMARK. UNIV AMSTERDAM,ACAD MED CTR,DEPT PULMONOL,1105 AZ AMSTERDAM,NETHERLANDS. NIH,WARREN G MAGNUSON CLIN CTR,DEPT CRIT CARE MED,BETHESDA,MD 20892. RP BENFIELD, TL (reprint author), UNIV COPENHAGEN,HVIDOVRE HOSP,DEPT INFECT DIS 144,DK-2650 HVIDOVRE,DENMARK. NR 27 TC 26 Z9 29 U1 0 U2 0 PU W B SAUNDERS CO LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0954-6111 J9 RESP MED JI Respir. Med. PD APR PY 1995 VL 89 IS 4 BP 285 EP 290 DI 10.1016/0954-6111(95)90089-6 PG 6 WC Cardiac & Cardiovascular Systems; Respiratory System SC Cardiovascular System & Cardiology; Respiratory System GA QU714 UT WOS:A1995QU71400006 PM 7597268 ER PT J AU ROBERTS, B SCHOOLER, C HOMMER, D COHEN, R AF ROBERTS, B SCHOOLER, C HOMMER, D COHEN, R TI CONFIRMATORY FACTOR-ANALYSIS OF THE NEUROLOGICAL EVALUATION SCALE SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,SOCIOENVIRONM STUDIES LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 20 EP 20 DI 10.1016/0920-9964(95)95074-J PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200053 ER PT J AU GEJMAN, PV GELERNTER, J CRAVCHIK, A GERSHON, ES PICKAR, D AF GEJMAN, PV GELERNTER, J CRAVCHIK, A GERSHON, ES PICKAR, D TI TESTING PATHOPHYSIOLOGIC HYPOTHESES OF PSYCHIATRIC-ILLNESS BY MOLECULAR-GENETIC APPROACHES SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,CNG,MOLEC CLIN INVEST UNIT,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 39 EP 39 DI 10.1016/0920-9964(95)95126-T PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200101 ER PT J AU INGRAHAM, LJ AF INGRAHAM, LJ TI PREVALENCE OF DSM-IV CRITERIA FOR SCHIZOTYPAL PERSONALITY-DISORDER AMONG THE BIOLOGICAL RELATIVES OF ADOPTEES WITH CHRONIC-SCHIZOPHRENIA SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,PSYCHOL & PSYCHOPATHOL LAB,INTRAMURAL RES PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 41 EP 41 DI 10.1016/0920-9964(95)95133-T PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200109 ER PT J AU HITRI, A WYATT, RJ AF HITRI, A WYATT, RJ TI DECREASED DOPAMINE TRANSPORTER RECEPTORS IN THE ANTERIOR CINGULATE CORTEX OF SCHIZOPHRENIC-PATIENTS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 DEPT VET AFFAIRS MED CTR,WASHINGTON,DC 20422. GEORGETOWN UNIV,SCH MED,WASHINGTON,DC 20422. NIMH,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20422. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 59 EP 60 DI 10.1016/0920-9964(95)95188-F PG 2 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200164 ER PT J AU LIPSKA, BK LILLRANK, SM WEINBERGER, DR AF LIPSKA, BK LILLRANK, SM WEINBERGER, DR TI DISRUPTION OF CORTICAL AND SUBCORTICAL FUNCTION FOLLOWING DEVELOPMENTAL HIPPOCAMPAL DAMAGE - A RAT MODEL OF SCHIZOPHRENIA SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,INTRAMURAL RES PROGRAM,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 65 EP 65 DI 10.1016/0920-9964(95)95204-M PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200180 ER PT J AU NATSUKARI, N WYATT, RJ BAKER, I TORREY, EF KULAGA, H MASSERANO, JM AF NATSUKARI, N WYATT, RJ BAKER, I TORREY, EF KULAGA, H MASSERANO, JM TI ALTERED CYCLIC-AMP RESPONSE TO FORSKOLIN AND CHOLERA-TOXIN AFTER PROTEIN-KINASE-C ACTIVATION IN EBV-TRANSFORMED B-LYMPHOCYTES FROM SCHIZOPHRENICS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,ST ELIZABETHS HOSP,CTR NEUROSCI,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 67 EP 67 DI 10.1016/0920-9964(95)95212-R PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200186 ER PT J AU BREIER, A CARSON, R ECKELMAN, W DEBARTOLOMEIS, A SAUNDERS, RC WEINBERGER, D SU, TP PICKAR, D AF BREIER, A CARSON, R ECKELMAN, W DEBARTOLOMEIS, A SAUNDERS, RC WEINBERGER, D SU, TP PICKAR, D TI IN-VIVO ESTIMATES OF SYNAPTIC DOPAMINE CONCENTRATIONS WITH C-11 RACLOPRIDE/PET - A DIRECT TEST OF THE DOPAMINE HYPOTHESIS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 76 EP 77 DI 10.1016/0920-9964(95)95240-A PG 2 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200216 ER PT J AU BREIER, A MALHOTRA, A PINALS, D CHUNG, IW HIDARY, J SU, TP HSIAO, J PICKAR, D AF BREIER, A MALHOTRA, A PINALS, D CHUNG, IW HIDARY, J SU, TP HSIAO, J PICKAR, D TI NEUROANATOMICAL LOCALIZATION OF NMDA RECEPTOR-MEDIATED PSYCHOSIS IN HEALTHY CONTROLS AND SCHIZOPHRENIC-PATIENTS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 77 EP 77 DI 10.1016/0920-9964(95)95242-2 PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200217 ER PT J AU ELKASHEF, AM DOUDET, D COHEN, RM WYATT, RJ AF ELKASHEF, AM DOUDET, D COHEN, RM WYATT, RJ TI PRESYNAPTIC DOPAMINE FUNCTION IN SCHIZOPHRENIA - AN F-18 DOPA PET STUDY SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 82 EP 82 DI 10.1016/0920-9964(95)95257-A PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200231 ER PT J AU HSIAO, JK CHUNG, IW COHEN, R PICKAR, D AF HSIAO, JK CHUNG, IW COHEN, R PICKAR, D TI REGIONALLY SPECIFIC CHANGES IN BRAIN GLUCOSE-METABOLISM ASSOCIATION WITH RESPONSE TO CLOZAPINE TREATMENT SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 85 EP 86 DI 10.1016/0920-9964(95)95266-C PG 2 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200242 ER PT J AU KNABLE, MB GONZALEZ, J COPPOLA, R JONES, DW NAWROZ, S GOREY, J WEINBERGER, DR AF KNABLE, MB GONZALEZ, J COPPOLA, R JONES, DW NAWROZ, S GOREY, J WEINBERGER, DR TI I-123 IBZM SPECT IN NEUROLEPTIC-FREE SCHIZOPHRENIC-PATIENTS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 88 EP 88 DI 10.1016/0920-9964(95)95274-D PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200250 ER PT J AU NOGA, JT BARTLEY, AB JONES, DW TORREY, EF WEINBERGER, DR AF NOGA, JT BARTLEY, AB JONES, DW TORREY, EF WEINBERGER, DR TI CORTICAL GYRAL ANATOMY AND GROSS BRAIN DIMENSIONS IN MONOZYGOTIC TWINS DISCORDANT FOR SCHIZOPHRENIA EXAMINED WITH 3-D MRI SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,DIRP,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 93 EP 93 DI 10.1016/0920-9964(95)95289-L PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200266 ER PT J AU PICKAR, D SU, TP COPPOLA, R LEE, CS HSIAO, JK BREIER, A WEINBERGER, DR AF PICKAR, D SU, TP COPPOLA, R LEE, CS HSIAO, JK BREIER, A WEINBERGER, DR TI DA OCCUPANCY AND DOPAMINE RELEASE DETERMINED BY I-123 IBZM SPECT FOLLOWING CLOZAPINE DOSE REDUCTION SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 0 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 96 EP 96 DI 10.1016/0920-9964(95)95298-N PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200274 ER PT J AU KOTRLA, KJ MATTAY, VS NAWROZ, S SEXTON, RH SANTHA, AKS VANGELDEREN, P DUYN, JH MOONEN, CTW FRANK, JA WEINBERGER, DR AF KOTRLA, KJ MATTAY, VS NAWROZ, S SEXTON, RH SANTHA, AKS VANGELDEREN, P DUYN, JH MOONEN, CTW FRANK, JA WEINBERGER, DR TI FUNCTIONAL MAGNETIC-RESONANCE-IMAGING IN NORMAL CONTROLS AND SCHIZOPHRENICS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. RI Duyn, Jozef/F-2483-2010; Moonen, Chrit/K-4434-2016 OI Moonen, Chrit/0000-0001-5593-3121 NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 103 EP 103 DI 10.1016/0920-9964(95)95321-Y PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200296 ER PT J AU GOLD, J CARPENTER, C RANDOLPH, C GOLDBERG, T WEINBERGER, D AF GOLD, J CARPENTER, C RANDOLPH, C GOLDBERG, T WEINBERGER, D TI AUDITORY WORKING-MEMORY AND THE WISCONSIN CARD SORTING TEST SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,NEUROSCI CTR ST ELIZABETHS,WASHINGTON,DC 20032. NR 0 TC 1 Z9 1 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 117 EP 118 DI 10.1016/0920-9964(95)95361-C PG 2 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200337 ER PT J AU GOLDBERG, TE GOLD, JM TORREY, EF WEINBERGER, DR AF GOLDBERG, TE GOLD, JM TORREY, EF WEINBERGER, DR TI SEX-DIFFERENCES AND NEUROCOGNITION IN SCHIZOPHRENIA SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,NEUROSCI CTR ST ELIZABETHS,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 118 EP 118 DI 10.1016/0920-9964(95)95363-E PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200338 ER PT J AU SCHOOLER, C ROBERTS, B COHEN, R AF SCHOOLER, C ROBERTS, B COHEN, R TI OCULAR, COGNITIVE AND SOCIAL INTERFERENCE IN SCHIZOPHRENIA SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,SOCIOENVIRONM STUDIES LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 133 EP 133 DI 10.1016/0920-9964(95)95412-3 PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200389 ER PT J AU BREIER, A AF BREIER, A TI NEGATIVE SYMPTOMS AND NORADRENERGIC FUNCTION SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 143 EP 144 DI 10.1016/0920-9964(95)95445-F PG 2 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200421 ER PT J AU MALHOTRA, AK WEINGARTNER, H SIROCCO, K PINALS, D MISSAR, CD PICKAR, D BREJER, A AF MALHOTRA, AK WEINGARTNER, H SIROCCO, K PINALS, D MISSAR, CD PICKAR, D BREJER, A TI THE COGNITIVE EFFECTS OF KETAMINE, AN NMDA ANTAGONIST, IN NORMAL CONTROLS AND DRUG-FREE SCHIZOPHRENIC-PATIENTS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 158 EP 158 DI 10.1016/0920-9964(95)95491-Q PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200467 ER PT J AU NOGA, JT RODEFFER, CJ WEINBERGER, DR KLEINMAN, JE AF NOGA, JT RODEFFER, CJ WEINBERGER, DR KLEINMAN, JE TI COMPARISON OF POLYDIPSIC BEHAVIOR IN SCHIZOPHRENIA-PATIENTS ON CLOZAPINE VERSUS HALOPERIDOL SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,DIRP,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 160 EP 160 DI 10.1016/0920-9964(95)95496-V PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200472 ER PT J AU PICKAR, D AF PICKAR, D TI MECHANISM OF ACTION OF CLOZAPINE - IMPLICATIONS FOR A NEW-GENERATION OF ANTIPSYCHOTICS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 161 EP 161 DI 10.1016/0920-9964(95)95499-Y PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200476 ER PT J AU PINALS, DA MALHOTRA, AK MISSAR, CD BREIER, A PICKAR, D AF PINALS, DA MALHOTRA, AK MISSAR, CD BREIER, A PICKAR, D TI THE ROLE OF GENDER IN THE PHARMACOTHERAPY OF SCHIZOPHRENIA SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 161 EP 161 DI 10.1016/0920-9964(95)95501-Y PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200477 ER PT J AU SU, TP TUSKAN, J TSAO, L PICKAR, D AF SU, TP TUSKAN, J TSAO, L PICKAR, D TI AGGRESSION DURING DRUG-FREE AND ANTIPSYCHOTIC TREATMENT IN INPATIENTS WITH CHRONIC-SCHIZOPHRENIA, USING THE OVERT AGGRESSION SCALE SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 166 EP 166 DI 10.1016/0920-9964(95)95515-B PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200492 ER PT J AU WYATT, RJ AF WYATT, RJ TI EARLY INTERVENTION IN SCHIZOPHRENIA IMPROVES THE LONG-TERM COURSE OF THE ILLNESS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH,NEUROSCI CTR ST ELIZABETHS,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. NR 1 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 170 EP 170 DI 10.1016/0920-9964(95)95528-H PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200503 ER PT J AU TORREY, EF RAWLINGS, R AF TORREY, EF RAWLINGS, R TI BIRTH SEASONALITY IN BIPOLAR DISORDER, SCHIZOPHRENIA, SCHIZOAFFECTIVE DISORDER, AND STILLBIRTHS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,WASHINGTON,DC 20032. NR 0 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 199 EP 200 DI 10.1016/0920-9964(95)95617-I PG 2 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200593 ER PT J AU TORREY, EF AF TORREY, EF TI IS SCHIZOPHRENIA INCREASING IN THE UNITED-STATES SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,WASHINGTON,DC 20032. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 200 EP 200 DI 10.1016/0920-9964(95)95618-J PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200594 ER PT J AU LU, YY BLAIR, DG AF LU, YY BLAIR, DG TI STABLE ONCOGENIC TRANSFORMATION-INDUCED BY MICROCELL-MEDIATED GENE-TRANSFER SO SCIENCE IN CHINA SERIES B-CHEMISTRY LIFE SCIENCES & EARTH SCIENCES LA English DT Article DE ONCOGENE; MICROCELL; CELL TRANSFORMATION; GENE MAPPING; MET ID INSITU HYBRIDIZATION; TYROSINE KINASE; CYSTIC-FIBROSIS; CELLS; MET; CHROMOSOME; ACTIVATION; SEQUENCES; HYBRIDS AB Oncogenes have been identified using DNA-mediated transfection, but the size of the transferable and unrearranged DNA gene rearrangement and amplification which occur during the transfection process limit the use of the techniques. We have evaluated microcell-mediated gene transfer techniques for the transfer and analysis of dominant oncogenes. MNNG-HOS, a transformed human cell line which contained the met oncogene mapping to human chromosome 7 was infected with retroviruses carrying drug resistance markers and used to optimize microcell preparation and transfer. Stable and drug-resistant hybrids containing single human chromosomes as well as the fod of the transformed cells containing the activated met oncogene and intact human chromosomes were obtained. Hybridization analysis with probes (i.e. collA2 pJ3.11) mapping up to 1 Mb away from met shows that the cells from the individual foci contain different amounts of apparently unrearranged human DNA associated with the oncogene, and the microcell-generated transformants retain more distal markers than those observed in either DNA- or chromosome-mediated transfers. In conjunction with other techniques, microcell fusion should be useful for gene mapping as well as the study of gene function and expression in cell transformation and malignancy. C1 NCI,MOLEC ONCOL LAB,FREDERICK,MD 21702. RP LU, YY (reprint author), BEIJING INST CANC RES,BEIJING 100034,PEOPLES R CHINA. NR 18 TC 0 Z9 1 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 1001-652X J9 SCI CHINA SER B JI Sci. China Ser. B-Chem. Life Sci. Earth Sci. PD APR PY 1995 VL 38 IS 4 BP 448 EP 456 PG 9 WC Chemistry, Multidisciplinary SC Chemistry GA QY027 UT WOS:A1995QY02700008 PM 7786413 ER PT J AU WRIGHT, LL MCNELLIS, D AF WRIGHT, LL MCNELLIS, D TI NATIONAL-INSTITUTE-OF-CHILD-HEALTH-AND-HUMAN-DEVELOPMENT (NICHD)-SPONSORED PERINATAL RESEARCH NETWORKS SO SEMINARS IN PERINATOLOGY LA English DT Review ID RESPIRATORY-DISTRESS SYNDROME; SYNTHETIC SURFACTANT; TRIAL; BIRTH; MULTICENTER; PHYSICIANS; INFANTS; GRAMS RP WRIGHT, LL (reprint author), NICHHD,RM 4B03F,6100 EXECUT BLVD,ROCKVILLE,MD 20852, USA. NR 36 TC 6 Z9 6 U1 0 U2 3 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0146-0005 J9 SEMIN PERINATOL JI Semin. Perinatol. PD APR PY 1995 VL 19 IS 2 BP 112 EP 123 DI 10.1016/S0146-0005(05)80031-X PG 12 WC Obstetrics & Gynecology; Pediatrics SC Obstetrics & Gynecology; Pediatrics GA QV878 UT WOS:A1995QV87800003 PM 7604302 ER PT J AU COLE, GW SINDELAR, WF AF COLE, GW SINDELAR, WF TI IATROGENIC TRANSPLANTATION OF OSTEOSARCOMA SO SOUTHERN MEDICAL JOURNAL LA English DT Note ID METASTASES AB We report a case in which a patient having curettage and resection of a presumed benign lesion of the tibia (later recognized as osteosarcoma) had probable iatrogenic transplantation of tumor to the contralateral iliac crest, which had served as a donor site for bone chips used to pack the tibial lesion. The patient later had above-knee amputation for the primary tumor and eventually required contralateral hemipelvectomy when tumor developed in the iliac crest donor site. We discuss the literature of tumor transplantation and seeding in operative settings and stress the clinical importance of avoiding possible tumor contamination of operative fields by meticulous instrument changes and by isolation of multiple surgical fields. C1 NCI,SURG BRANCH,BETHESDA,MD 20892. NR 12 TC 13 Z9 14 U1 0 U2 0 PU SOUTHERN MEDICAL ASSN PI BIRMINGHAM PA 35 LAKESHORE DR PO BOX 190088, BIRMINGHAM, AL 35219 SN 0038-4348 J9 SOUTHERN MED J JI South.Med.J. PD APR PY 1995 VL 88 IS 4 BP 485 EP 488 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA QT269 UT WOS:A1995QT26900023 PM 7716608 ER PT J AU POTEMBER, RS MATSUZAWA, M LIESI, P AF POTEMBER, RS MATSUZAWA, M LIESI, P TI CONDUCTING NETWORKS FROM CULTURED-CELLS ON SELF-ASSEMBLED MONOLAYERS SO SYNTHETIC METALS LA English DT Article; Proceedings Paper CT International Conference on Science and Technology of Synthetic Metals (ICSM 94) CY JUL 24-29, 1994 CL SEOUL, SOUTH KOREA ID COPLANAR MOLECULAR ASSEMBLIES; GROWTH; OUTGROWTH AB A combination of photolithographic and chemical techniques were used to prepare biologically active patterned substrates to guide the development of neurons in culture. A synthetic peptide, derived from the B2 chain of laminin was chemically attached to patterned silicon surfaces to promote the development and guidance of embryonic rat hippocampal neurons. On parallel lines, neurons developed a mature bipolar morphology. These modified surfaces may be an important element of future biosensors and neural prosthetic devices. C1 NIAAA,MOLEC & CELLULAR NEUROBIOL LAB,ROCKVILLE,MD 20852. RP POTEMBER, RS (reprint author), JOHNS HOPKINS UNIV,APPL PHYS LAB,LAUREL,MD 20723, USA. NR 8 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE SA PI LAUSANNE PA PO BOX 564, 1001 LAUSANNE, SWITZERLAND SN 0379-6779 J9 SYNTHETIC MET JI Synth. Met. PD APR 1 PY 1995 VL 71 IS 1-3 BP 1997 EP 1999 DI 10.1016/0379-6779(94)03137-U PG 3 WC Materials Science, Multidisciplinary; Physics, Condensed Matter; Polymer Science SC Materials Science; Physics; Polymer Science GA QT326 UT WOS:A1995QT32600195 ER PT J AU GOMEZ, DE NASON, AM THORGEIRSSON, UP AF GOMEZ, DE NASON, AM THORGEIRSSON, UP TI THROMBIN TREATMENT OF ENDOTHELIAL-CELLS STIMULATES ADHESION OF ONCOGENE-TRANSFORMED BUT NOT PARENT RAT-LIVER EPITHELIAL-CELLS SO THROMBOSIS RESEARCH LA English DT Note DE THROMBIN; ADHESION; ENDOTHELIAL CELLS; TUMOR CELLS ID METASTASIS INVIVO; TUMOR-CELLS; INVITRO; NEUTROPHILS; PLATELETS; PROTEINS; RECEPTOR; BINDING; GMP-140 RP GOMEZ, DE (reprint author), NCI,DIV CANC ETIOL,OFF DIRECTOR,BLDG 37,ROOM 2D-02,BETHESDA,MD 20892, USA. OI Gomez, Daniel E/0000-0002-8629-0787 NR 21 TC 1 Z9 1 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0049-3848 J9 THROMB RES JI Thromb. Res. PD APR 1 PY 1995 VL 78 IS 1 BP 87 EP 94 DI 10.1016/0049-3848(95)00037-2 PG 8 WC Hematology; Peripheral Vascular Disease SC Hematology; Cardiovascular System & Cardiology GA QN155 UT WOS:A1995QN15500007 PM 7778069 ER PT J AU CUNNINGHAM, ML PIPPIN, LL ANDERSON, NL WENK, ML AF CUNNINGHAM, ML PIPPIN, LL ANDERSON, NL WENK, ML TI THE HEPATOCARCINOGEN METHAPYRILENE BUT NOT THE ANALOG PYRILAMINE INDUCES SUSTAINED HEPATOCELLULAR REPLICATION AND PROTEIN ALTERATIONS IN F344 RATS IN A 13-WEEK FEED STUDY SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID CELL-PROLIFERATION; GENOTOXIC CARCINOGENESIS; CHEMICAL CARCINOGENESIS; DNA-SYNTHESIS; LIVER-TUMORS; TOXICITY; HYDROCHLORIDE; MUTAGENICITY; EXPOSURE; RODENTS AB Methapyrilene (MPH) was a widely used antihistamine until it was found to produce hepatocellular carcinoma and cholangiocarcinoma in Fischer 344 rats. The structurally similar antihistamine pyrilamine (PYR) was marginally or noncarcinogenic in a similar study. The peroxisome proliferator Wy-14,643 was included in this study as a positive control. As part of a program to investigate the mechanisms whereby structurally similar chemicals produce different toxicities, we studied these three chemicals for the induction of cell proliferation in the liver of F344 rats. Male rats were treated for up to 13 weeks with feed dosed with MPH (HCl salt) at 0, 50, 100, 250, or 1000 ppm or PYR (maleate salt) at 1000 ppm to duplicate the route of administration and high-dose groups used in the carcinogenesis assay. In addition, the nongenotoxic hepatocarcinogen peroxisome proliferator Wy-14,643 was included as a positive cell-proliferating chemical. Cell proliferation was quantitated by measuring the incorporation of bromodeoxyuridine (BrDU) administered by osmotic minipump for 7 days and the appearance of proliferating cell nuclear antigen (PCNA) immunohistochemically. The BrDU-labeling index showed a large and sustained increase in rats treated with MPH at 250 and 1000 ppm, sustaining greater than 50% labeling in the higher dose group of 4-, 6-, and 13-week treatment groups. PYR at 1000 ppm demonstrated no significant increase in labeling above control levels at any time point. PCNA-labeling indexes showed similar but reduced increases for MPH and were comparable to control for the PYR dose groups. Two-dimensional gel electrophoresis was used for the detection of quantitative changes in gene expression and qualitative changes in the charges of specific mitochondrial and cytosolic proteins. Quantitative changes in 32 proteins induced by MPH and 39 changes induced by Wy-14,643 were detected throughout the 13-week study. Specific mitochondrial protein charge shifts were associated with high-dose MPH treatment that were not observed in animals treated with Wy-14,643. PYR induced no significant qualitative or quantitative protein alterations. Hepatocellular proliferation of the large magnitude observed following dietary administration of MPH, and not PYR may contribute to the mechanism of carcinogenesis of MPH. (C) 1995 Academic Press, Inc. C1 PATHOL ASSOCIATES INC,FREDERICK,MD 21701. LARGE SCALE BIOL CORP,ROCKVILLE,MD 20850. MICROBIOL ASSOCIATES INC,ROCKVILLE,MD 20850. RP CUNNINGHAM, ML (reprint author), NIEHS,CHEM BRANCH,POB 12233,MAIL DROP B3-10,RES TRIANGLE PK,NC 27709, USA. NR 38 TC 35 Z9 35 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD APR PY 1995 VL 131 IS 2 BP 216 EP 223 DI 10.1006/taap.1995.1064 PG 8 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA QU176 UT WOS:A1995QU17600005 PM 7716764 ER PT J AU KAYAMA, F YOSHIDA, T ELWELL, MR LUSTER, MI AF KAYAMA, F YOSHIDA, T ELWELL, MR LUSTER, MI TI ROLE OF TUMOR-NECROSIS-FACTOR-ALPHA IN CADMIUM-INDUCED HEPATOTOXICITY SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID LIVER MACROPHAGES; GENE-EXPRESSION; ACETAMINOPHEN HEPATOTOXICITY; ACTIVATED MACROPHAGES; ALCOHOLIC HEPATITIS; ENDOTHELIAL-CELLS; POTENTIAL ROLE; KUPFFER CELLS; INTERLEUKIN-1; MICE AB Liver and kidney injury following acute or chronic exposure to cadmium is well characterized. While hepatocytes and endothelial cells of the sinusoids are thought to be the primary cellular targets in the liver, ultrastructural changes may vary depending upon the exposure regimen and the time following administration. Since acute and chronic liver disease is often associated with the presence of cytokines, we investigated the role of proinflammatory cytokines in cadmium-induced hepatotoxicity. Supernatants from cultured liver slices obtained from acute or subchronic cadmium-exposed rats and mice were collected and cytokine secretion was examined. In addition, mRNA transcripts for IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, MIP-2, IFN-gamma, and ICAM-1 from livers of treated mice were quantitated by reverse transcription-polymerase chain reaction. Modest increases in secretion of TNF-alpha, IL-1 alpha, and IL-6 were observed in response to cadmium which were enhanced in LPS-primed mice. Additionally, cadmium exposure increased IL-1 alpha, IL-1 beta, TNF-alpha, MIP-2, IL-6, and ICAM-1 mRNA transcripts in the liver. Immunohistochemical analysis revealed that TNF-alpha was associated with nonparenchymal cells in livers of cadmium-treated mice. Cadmium exposure produced a marked increase in plasma hepatocellular enzyme levels (i.e., AST, LDH, SDH), acute phase proteins (i.e., serum amyloid A), and foci formation in the liver, while focal inflammation and serum amyloid A (SAA) secretion, but not plasma enzymes, were further increased in cadmium-exposed mice primed with LPS. SAA secretion and focal inflammation were prevented by pretreatment with antibodies to TNF-alpha, indicating that these pathological manifestations are cytokine dependent. These data indicate that TNF-alpha, released from nonparenchymal cells as well as associated cytokines, are responsible for certain manifestations observed with cadmium-induced hepatotoxicity. C1 NIEHS,ENVIRONM TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. UNIV OCCUPAT & ENVIRONM HLTH,DEPT ENVIRONM HLTH,KITAKYUSHU,FUKUOKA 807,JAPAN. TOKAI UNIV,SCH MED,DEPT ENVIRONM HLTH,ISEHARA,KANAGAWA 25911,JAPAN. RP KAYAMA, F (reprint author), NIEHS,ENVIRONM IMMUNOL & NEUROBIOL SECT,RES TRIANGLE PK,NC 27709, USA. NR 45 TC 136 Z9 136 U1 0 U2 8 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD APR PY 1995 VL 131 IS 2 BP 224 EP 234 DI 10.1006/taap.1995.1065 PG 11 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA QU176 UT WOS:A1995QU17600006 PM 7536360 ER PT J AU HOLLADAY, SD SMITH, BJ LUSTER, MI AF HOLLADAY, SD SMITH, BJ LUSTER, MI TI B-LYMPHOCYTE PRECURSOR CELLS REPRESENT SENSITIVE TARGETS OF T2 MYCOTOXIN EXPOSURE SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID DIETARY T-2 TOXIN; THYMIC ATROPHY; MICE; INFECTION; RESISTANCE; TOXICITY; EXPRESSION; FUSARIUM; DNA AB Exposure of experimental animals and humans to the Fusarium trichothecene metabolite, T2 toxin, has been associated with a variety of immunosuppressive effects, including altered parameters of humoral-mediated immunity. Although T2 toxin is cytotoxic in vitro to lymphocytic cells, limited information is presently available regarding the contribution of such a mechanism to immunosuppression in vivo, or to potential immune cell targets. In the present report, subchronic T2 toxin treatment of timed-pregnant B6C3F1 mice resulted in significant and selective depletion of fetal liver cells expressing low levels of surface CD44 and CD45 antigens, suggestive of possible lymphoid progenitor cell sensitivity to this agent. Evaluation of CD45R antigen expression in fetal liver supported such a hypothesis, demonstrating a significant reduction in fetal liver B lymphocytic cells in animals exposed to T2 toxin. Subsequent in vitro T2 toxin exposure of fetal liver cells enriched for prolymphocytes by differential density gradient centrifugation demonstrated the presence of a highly sensitive subpopulation of cells that was eliminated in a selective, and near-complete, manner by T2 toxin exposure. This sensitive cell population was observed to have light-scatter characteristics of CD45R(+) B-lineage lymphocytes. Additional studies in adult mice demonstrated a reduction in CD44(lo) and CD45R(+) bone marrow cells similar to that seen in fetal liver, indicating that T2 toxin may also target immature B lymphocytes in this hematopoietic compartment. Taken together, these data suggest that the precursors of B cells may represent, for unknown reasons, highly sensitive targets of T2 toxin exposure. (C) 1995 Academic Press, Inc. C1 NIEHS,ENVIRONM IMMUNOL & MICROBIOL SECT,RES TRIANGLE PK,NC 27709. RP HOLLADAY, SD (reprint author), VIRGINIA POLYTECH INST & STATE UNIV,VIRGINIA MARYLAND REG COLL VET MED,BLACKSBURG,VA 24061, USA. NR 31 TC 26 Z9 27 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD APR PY 1995 VL 131 IS 2 BP 309 EP 315 DI 10.1006/taap.1995.1073 PG 7 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA QU176 UT WOS:A1995QU17600014 PM 7716771 ER PT J AU GRIFFIN, RJ BURKA, LT CUNNINGHAM, ML AF GRIFFIN, RJ BURKA, LT CUNNINGHAM, ML TI ACTIVITY OF HEPATIC DRUG-METABOLIZING-ENZYMES FOLLOWING OXAZEPAM-DOSED FEED TREATMENT IN B6C3F1 MICE SO TOXICOLOGY LETTERS LA English DT Article DE OXAZEPAM; BENZODIAZEPINE; ENZYME INDUCTION; CYTOCHROME P450 ID MOUSE-LIVER; RAT-LIVER; INDUCTION; DIAZEPAM; SERIES AB Oxazepam has been determined to be a potent hepatocarcinogen in mice. Evidence in the literature indicates that oxazepam is capable of inducing drug metabolizing enzymes in rodents and an association between enzyme induction and carcinogenesis has been proposed for other compounds such as phenobarbital. We examined the pattern of enzyme induction that occurs under bioassay conditions in male B6C3F1 mice. The results indicate that oxazepam is capable of inducing multiple drug metabolizing enzymes under bioassay conditions. Closer examination of the most induced samples suggests that oxazepam is a phenobarbital-type enzyme inducer. RP GRIFFIN, RJ (reprint author), NIEHS,CHEM BRANCH,MD C3-02,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 25 TC 12 Z9 12 U1 0 U2 0 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0378-4274 J9 TOXICOL LETT JI Toxicol. Lett. PD APR PY 1995 VL 76 IS 3 BP 251 EP 256 DI 10.1016/0378-4274(95)80010-B PG 6 WC Toxicology SC Toxicology GA QX651 UT WOS:A1995QX65100009 PM 7762012 ER PT J AU JEFFERSON, WN SHIGETA, H NEWBOLD, RR AF JEFFERSON, WN SHIGETA, H NEWBOLD, RR TI ENHANCED CHEMILUMINESCENT DETECTION OF FLUORESCEIN-LABELED NUCLEIC-ACIDS COMPARED TO P-32 LABELING METHODS - LACTOFERRIN AS A MARKER SO TOXICOLOGY METHODS LA English DT Article DE NORTHERN BLOTTING; ENHANCED CHEMILUMINESCENCE (ECL); FLUORESCEIN RANDOM PRIME LABELING; LACTOFERRIN (LF); P-32 (P-32) ID REPRODUCTIVE-TRACT; RIBONUCLEIC-ACID; MOUSE; PROTEIN AB This study evaluated the ability of nonradioactive, sensitive Northern blotting methods to detect small amounts of messenger RNA. The more traditional P-32 labeling and detection method was compared to enhanced chemiluminescent (ECL) detection of fluorescein labeled nucleic acids, The results show that the ECL method is equally as sensitive as P-32, and both methods are highly quantitative by image analysis. The fluorescein generated probes can be stored for several months as compared to several days storage for P-32 labeled probes. The ECL method of detecting fluorescein labeled nucleic acid probes should prove useful for many applications in biochemistry, molecular biology, and toxicology. C1 NIEHS,ENVIRONM TOXICOL PROGRAM,TOXICOL BRANCH,REPROD TOXICOL GRP,RES TRIANGLE PK,NC 27709. NIEHS,REPROD LAB,DEV ENDOCRINOL & PHARMACOL SECT,RES TRIANGLE PK,NC 27709. NIEHS,DIV INTRAMURAL RES,DEV TOXICOL ENVIRONM BIOL & MED PROGRAM,RES TRIANGLE PK,NC 27709. NR 8 TC 2 Z9 2 U1 0 U2 0 PU TAYLOR & FRANCIS PI BRISTOL PA 1900 FROST ROAD, SUITE 101, BRISTOL, PA 19007-1598 SN 1051-7235 J9 TOXICOL METHOD JI Toxicol. Method. PD APR-JUN PY 1995 VL 5 IS 2 BP 81 EP 87 DI 10.3109/15376519509045903 PG 7 WC Toxicology SC Toxicology GA RV750 UT WOS:A1995RV75000002 ER PT J AU LENFANT, C AF LENFANT, C TI NEW GENETIC APPROACHES - ESTABLISHING RESOURCES FOR RESEARCH SO TRANSFUSION LA English DT Note RP LENFANT, C (reprint author), NHLBI,BLDG 31,ROOM 5A52,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 SN 0041-1132 J9 TRANSFUSION JI Transfusion PD APR PY 1995 VL 35 IS 4 BP 346 EP 347 DI 10.1046/j.1537-2995.1995.35495216085.x PG 2 WC Hematology SC Hematology GA QQ300 UT WOS:A1995QQ30000012 PM 7701554 ER PT J AU CANNON, GW REMMERS, EF WILDER, RL HIBBS, JB GRIFFITHS, MM AF CANNON, GW REMMERS, EF WILDER, RL HIBBS, JB GRIFFITHS, MM TI NITRIC-OXIDE PRODUCTION DURING ADJUVANT-INDUCED ARTHRITIS IS ASSOCIATED WITH TUMOR-NECROSIS-FACTOR GENOTYPE SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 10th International Workshop on Alloantigenic Systems in the Rat CY AUG 23-26, 1994 CL HOKKAIDO UNIV, SAPPORO, JAPAN SP TRANSPLANTAT SOC HO HOKKAIDO UNIV C1 UNIV UTAH,DEPT MED,DIV RHEUMATOL,SALT LAKE CITY,UT 84112. UNIV UTAH,DEPT MED,DIV INFECT DIS,SALT LAKE CITY,UT 84112. NIAMSD,ARTHRITIS & RHEUMATISM BRANCH,INFLAMMATORY JOINT DIS SECT,BETHESDA,MD 20892. RP CANNON, GW (reprint author), VET AFFAIRS MED CTR,SALT LAKE CITY,UT 84148, USA. NR 4 TC 2 Z9 2 U1 0 U2 0 PU APPLETON & LANGE PI E NORWALK PA 25 VAN ZANT ST, E NORWALK, CT 06855 SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD APR PY 1995 VL 27 IS 2 BP 1543 EP 1544 PG 2 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA QT729 UT WOS:A1995QT72900020 PM 7725404 ER PT J AU KOONIN, EV AF KOONIN, EV TI A PROTEIN SPLICE-JUNCTION MOTIF IN HEDGEHOG FAMILY PROTEINS SO TRENDS IN BIOCHEMICAL SCIENCES LA English DT Note ID ARCHAEA DNA-POLYMERASE; ADENOSINE-TRIPHOSPHATASE; INTERVENING SEQUENCES; CATALYTIC SUBUNIT; GENE; ENDONUCLEASES; INTRONS; VMA1 RP KOONIN, EV (reprint author), NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20894, USA. NR 24 TC 40 Z9 40 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0968-0004 J9 TRENDS BIOCHEM SCI JI Trends Biochem.Sci. PD APR PY 1995 VL 20 IS 4 BP 141 EP 142 DI 10.1016/S0968-0004(00)88989-6 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QU082 UT WOS:A1995QU08200004 PM 7770912 ER PT J AU BOMAN, AL KAHN, RA AF BOMAN, AL KAHN, RA TI ARF PROTEINS - THE MEMBRANE TRAFFIC POLICE SO TRENDS IN BIOCHEMICAL SCIENCES LA English DT Review ID ADP-RIBOSYLATION FACTOR; GTP-BINDING-PROTEIN; GOLGI MEMBRANES; DEPENDENT BINDING; PHOSPHOLIPASE-D; BETA-COP; FAMILY; MYRISTOYLATION; DROSOPHILA; TRANSPORT AB Cofactor for cholera toxin; activator of phospholipase D; regulator of coat-protein assembly; inhibitor of membrane traffic; ability to cause expansion of the endoplasmic reticulum and vesiculation of the Golgi; sensitivity to membrane phospholipids - each of these activities has been attributed to Arf proteins. Can a single molecular mechanism link them all? RP BOMAN, AL (reprint author), NCI,DIV CANC TREATMENT,BIOL CHEM LAB,DEV THERAPEUT PROGRAM,BETHESDA,MD 20892, USA. NR 38 TC 223 Z9 228 U1 0 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0968-0004 J9 TRENDS BIOCHEM SCI JI Trends Biochem.Sci. PD APR PY 1995 VL 20 IS 4 BP 147 EP 150 DI 10.1016/S0968-0004(00)88991-4 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QU082 UT WOS:A1995QU08200006 PM 7770914 ER PT J AU HENGEN, PN AF HENGEN, PN TI METHODS AND REAGENTS - CARING FOR YOUR HYBRIDIZATION MEMBRANES SO TRENDS IN BIOCHEMICAL SCIENCES LA English DT Editorial Material RP HENGEN, PN (reprint author), NCI,FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21702, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0968-0004 J9 TRENDS BIOCHEM SCI JI Trends Biochem.Sci. PD APR PY 1995 VL 20 IS 4 BP 160 EP 161 DI 10.1016/S0968-0004(00)88994-X PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QU082 UT WOS:A1995QU08200013 PM 7770917 ER PT J AU LIPTON, SA BRENNEMAN, DE SILVERSTEIN, FS MASLIAH, E MUCKE, L AF LIPTON, SA BRENNEMAN, DE SILVERSTEIN, FS MASLIAH, E MUCKE, L TI GP120 AND NEUROTOXICITY IN-VIVO SO TRENDS IN PHARMACOLOGICAL SCIENCES LA English DT Letter ID ENVELOPE C1 CHILDRENS HOSP, BOSTON, MA 02115 USA. NICHHD, DEV & MOLEC PHARMACOL SECT, BETHESDA, MD 20892 USA. UNIV MICHIGAN, DEPT NEUROL, ANN ARBOR, MI 48109 USA. UNIV CALIF SAN DIEGO, DEPT NEUROSCI, LA JOLLA, CA 92093 USA. Scripps Res Inst, DEPT NEUROPHARMACOL, LA JOLLA, CA 92037 USA. RP LIPTON, SA (reprint author), HARVARD UNIV, SCH MED, DEPT NEUROL, BOSTON, MA 02115 USA. NR 10 TC 15 Z9 15 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0165-6147 J9 TRENDS PHARMACOL SCI JI Trends Pharmacol. Sci. PD APR PY 1995 VL 16 IS 4 BP 122 EP 122 DI 10.1016/S0165-6147(00)88998-1 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QY937 UT WOS:A1995QY93700003 PM 7610496 ER PT J AU HUNT, JA DISKO, MM BEHAL, SK LEAPMAN, RD AF HUNT, JA DISKO, MM BEHAL, SK LEAPMAN, RD TI ELECTRON-ENERGY-LOSS CHEMICAL IMAGING OF POLYMER PHASES SO ULTRAMICROSCOPY LA English DT Article; Proceedings Paper CT Workshop on Materials Science Opportunities for Field Emission Electron Sources CY JAN 05-08, 1994 CL SCOTTSDALE, AZ SP NATL SCI FDN, CTR HIGH RESOLUT ELECTRON MICROSCOPY, ARIZONA STATE UNIV, CTR SOLID STATE SCI ID POLYETHYLENE POLYSTYRENE BLENDS; BLOCK COPOLYMER; DIBLOCK COPOLYMER; MOLECULAR DESIGN; SPECTROSCOPY; HOMOPOLYMER; MORPHOLOGY; MICROSCOPY; SURFACES; SYSTEMS AB Transmission electron energy-loss spectrum-imaging investigations of a low-density polyethylene blend with polystyrene show that chemical imaging is possible with high spatial resolution for polymers in a dedicated scanning transmission electron microscope. Spectrum-imaging provides highly accurate phase identifications without staining. This polymer blend also contains a small amount of styrene-hydrogenated polyisoprene diblock copolymer (Kraton G 1701) as a compatibilizer. Detection of this compatibilizer at interfaces was expected to be difficult because of its chemical similarity to the pure components. We were unable to detect the copolymer with EELS imaging unambiguously, but were able to accurately map regions rich in either polyethylene or polystyrene. Chemical imaging techniques that we develop here are of use for polymer mixtures that exhibit variations in either carbon bonding or distribution of heteroatoms. C1 EXXON RES & ENGN CO,ANNANDALE,NJ 08801. NIH,BETHESDA,MD 20892. RP HUNT, JA (reprint author), GATAN INC,RES & DEV,PLEASANTON,CA 94588, USA. NR 30 TC 26 Z9 26 U1 0 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3991 J9 ULTRAMICROSCOPY JI Ultramicroscopy PD APR PY 1995 VL 58 IS 1 BP 55 EP 64 DI 10.1016/0304-3991(94)00178-P PG 10 WC Microscopy SC Microscopy GA QV106 UT WOS:A1995QV10600008 ER PT J AU CARTER, HB PEARSON, JD WACLAWIW, Z METTER, EJ CHAN, DW GUESS, HA WALSH, PC AF CARTER, HB PEARSON, JD WACLAWIW, Z METTER, EJ CHAN, DW GUESS, HA WALSH, PC TI PROSTATE-SPECIFIC ANTIGEN VARIABILITY IN MEN WITHOUT PROSTATE-CANCER - EFFECT OF SAMPLING INTERVAL ON PROSTATE-SPECIFIC ANTIGEN VELOCITY SO UROLOGY LA English DT Article AB Objectives. To evaluate short-term and long-term variability between prostate-specific antigen (PSA) measurements to determine the most appropriate PSA sampling interval and rate of PSA change (PSA velocity) to distinguish between men with and without prostate cancer. Methods. Retrospective study of PSA variability and PSA velocity in three groups of men without a diagnosis of prostate cancer and PSA levels less than 10 ng/mL: 56 men with a histologic diagnosis of benign prostatic hyperplasia (BPH; histologic BPH group) and 527 men with no history of cancer (noncancer group) who were part of the Baltimore Longitudinal Study of Aging and had PSA sampled at 2-year intervals (long-term), and 223 men with a clinical diagnosis of BPH (clinical BPH group) who had PSA sampled at 3-month intervals (short-term). PSA variability (deviation between consecutive measurements) and PSA velocity based on both two consecutive measurements and three consecutive measurements (average velocity) were calculated for each study group. Results. PSA velocity is the deviation in PSA measurements relative to the elapsed time between the measurements. Because the variability in PSA between measurements was similar for the groups, the major factors that influenced PSA velocity were the sampling interval between PSA measurements, and to a lesser extent, the number of repeat PSA measurements. The 99th percentile for PSA velocity was 0.7 (histologic BPH group) and 0.75 ng/mL per year for the noncancer group when three measurements with a 24-month PSA sampling interval were used. However, the 99th percentile for PSA velocity was 5.8 and 2.4 ng/mL per year when three measurements with 3-month and 6-month PSA sampling intervals were used. Using three measurements, the percentage of subjects with a PSA velocity more than 0.75 ng/mL per year was 1% for the groups with a 24-month PSA sampling interval and 28% and 17% for 3-month and 6-month PSA sampling intervals, respectively. The 99th percentile for PSA velocity and the percentage of subjects with a PSA velocity more than 0.75 ng/mL per year was higher using two measurements compared to three measurements regardless of PSA sampling interval. Conclusions. PSA velocity is inversely related to the interval between PSA measurements. A PSA velocity more than 0.75 ng/mL per year is useful in distinguishing between men with and without prostate cancer when: (1) velocity is based on three consecutive measurements; and (2) PSA is sampled long-term (2 years) but not short-term (3 to 6 months). C1 JOHNS HOPKINS UNIV HOSP,SCH MED,JAMES BUCHANAN BRADY UROL INST,DEPT PATHOL,BALTIMORE,MD 21287. NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. MERCK RES LABS,DEPT EPIDEMIOL,BLUE BELL,PA. RP CARTER, HB (reprint author), JOHNS HOPKINS UNIV HOSP,SCH MED,JAMES BUCHANAN BRADY UROL INST,DEPT UROL,403 MARBURG,BALTIMORE,MD 21287, USA. NR 10 TC 107 Z9 109 U1 0 U2 1 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0090-4295 J9 UROLOGY JI UROLOGY PD APR PY 1995 VL 45 IS 4 BP 591 EP 596 DI 10.1016/S0090-4295(99)80049-1 PG 6 WC Urology & Nephrology SC Urology & Nephrology GA QQ902 UT WOS:A1995QQ90200010 PM 7536366 ER PT J AU HSU, KHL CROWE, JE LUBECK, MD DAVIS, AR HUNG, PP CHANOCK, RM MURPHY, BR AF HSU, KHL CROWE, JE LUBECK, MD DAVIS, AR HUNG, PP CHANOCK, RM MURPHY, BR TI ISOLATION AND CHARACTERIZATION OF A HIGHLY ATTENUATED RESPIRATORY SYNCYTIAL VIRUS (RSV) VACCINE CANDIDATE BY MUTAGENESIS OF THE INCOMPLETELY ATTENUATED RSV A2 TS-1 NG-1 MUTANT VIRUS SO VACCINE LA English DT Article DE RESPIRATORY SYNCYTIAL VIRUS; LIVE VACCINE; VIRUS ATTENUATION ID TEMPERATURE-SENSITIVE MUTANT; F-GLYCOPROTEIN; COTTON RATS; CHILDREN; INFANTS; LIVE; IMMUNOGENICITY; INFECTION; EPITOPES; ADULTS AB Ts-1, a temperature sensitive (ts) mutant of RSV was previously derived from RSV A2 virus by mutagenesis with 5-fluorouracil (5-FU). Ts-1 was attenuated for adult volunteers and seropositive children but retained a low level of virulence in seronegative infant vaccinees as indicated by the occurrence of upper respiratory tract disease. Ts-1 NG-1, a more defective derivative of ts-1, has produced by mutagenesis of ts-1 with nitrosoguanidine. However, ts-1 NG-1 still retained a low level of virulence for the upper respiratory tract and showed some genetic instability in chimpanzees. With renewed interest in the goal of developing a live, attenuated RSV vaccine, we have now attempted to further attenuate ts-1 NG-1 by mutagenesis with 5-FU and 5-azacytidine. Four mutants that are phenotypically different from the ts-1 NG-1 parental virus were identified. Each of the four mutants was more restricted in replication in BALB/c mice compared with the ts-1 NG-1 parental virus. One of the ts-1 NG-1 derivatives, termed A-20-4, which showed the lowest (35 degrees C) in vitro shutoff temperature and which was also completely restricted in replication in BALB/c mice, was selected for further evaluation in seronegative chimpanzees, A-20-4 did not cause rhinorrhea in chimpanzees but induced detectable titers of serum RSV neutralizing antibodies in 2 of 4 chimpanzees. Apparent complete protection to subsequent challenge with, wild-type RSV was observed in each of the four chimpanzees previously immunized with A-20-4, The ts-1 NG-1 A-20-4 mutant thus represents a promising live attenuated RSV vaccine candidate. C1 NIAID,INFECT DIS LAB,BETHESDA,MD 20892. RP HSU, KHL (reprint author), WYETH AYERST RES,145-R-2,POB 8299,PHILADELPHIA,PA 19101, USA. RI Crowe, James/B-5549-2009 OI Crowe, James/0000-0002-0049-1079 NR 27 TC 10 Z9 10 U1 0 U2 2 PU BUTTERWORTH-HEINEMANN LTD PI OXFORD PA LINACRE HOUSE JORDAN HILL, OXFORD, OXON, ENGLAND OX2 8DP SN 0264-410X J9 VACCINE JI Vaccine PD APR PY 1995 VL 13 IS 5 BP 509 EP 515 DI 10.1016/0264-410X(94)00002-5 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA QR844 UT WOS:A1995QR84400013 PM 7543716 ER PT J AU TAYLOR, J MEIGNIER, B TARTAGLIA, J LANGUET, B VANDERHOEVEN, J FRANCHINI, G TRIMARCHI, C PAOLETTI, E AF TAYLOR, J MEIGNIER, B TARTAGLIA, J LANGUET, B VANDERHOEVEN, J FRANCHINI, G TRIMARCHI, C PAOLETTI, E TI BIOLOGICAL AND IMMUNOGENIC PROPERTIES OF A CANARYPOX-RABIES RECOMBINANT, ALVAC RG (VCP65) IN NON-AVIAN SPECIES SO VACCINE LA English DT Article DE POXVIRUS-BASED VACCINES; CANARYPOX VIRUS (ALVAC); ALVAC-RC(VCP65); SAFETY; IMMUNOGENICITY ID FOWLPOX VIRUS RECOMBINANT; VACCINIA VIRUS; PROTECTIVE IMMUNITY; FUSION PROTEIN; GLYCOPROTEIN; EXPRESSION; NYVAC; GENE AB A canarypox-based (ALVAC) recombinant expressing the rabies G glycoprotein has been utilized to assess in vitro and in vivo biological properties of the canarypox virus vector system. In vitro studies have shown that no replication of the virus can be detected on six human-derived cell lines, nor can the virus be readily adapted to replicate on nonavian cells. Expression of the rabies G can be detected on all cell lines analyzed in the absence of productive viral replication Analysis of viral-specific DNA accumulation indicated that the block in the replication cycle in the human cell lines analyzed occurred prior to DNA replication The exact nature of the block, however, remains unknown. The concept of using a non-replicating immunization vehicle has been demonstrated through extensive in vivo studies in a range of species including non-human primates and humans. The results of such in vivo studies have exemplified the safety and immunogenicity of the ALVAC vaccine vector. C1 PASTEUR MERIEUX SERUMS & VACCINS,F-69280 MARCY LETOILE,FRANCE. RHONE MERIEUX,LYON 07,FRANCE. NCI,TUMOR CELL BIOL LAB,BETHESDA,MD 20892. NEW YORK STATE DEPT HLTH,WADSWORTH CTR LABS & RES,GRIFFIN LABS,ALBANY,NY 12201. RP TAYLOR, J (reprint author), VIROGENET CORP,465 JORDAN RD RENSSELAER TECHNOL PK,TROY,NY 12180, USA. NR 28 TC 82 Z9 83 U1 1 U2 2 PU BUTTERWORTH-HEINEMANN LTD PI OXFORD PA LINACRE HOUSE JORDAN HILL, OXFORD, OXON, ENGLAND OX2 8DP SN 0264-410X J9 VACCINE JI Vaccine PD APR PY 1995 VL 13 IS 6 BP 539 EP 549 DI 10.1016/0264-410X(94)00028-L PG 11 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA RB419 UT WOS:A1995RB41900004 PM 7483774 ER PT J AU MEISTER, GE ROBERTS, CGP BERZOFSKY, JA DEGROOT, AS AF MEISTER, GE ROBERTS, CGP BERZOFSKY, JA DEGROOT, AS TI 2 NOVEL T-CELL EPITOPE PREDICTION ALGORITHMS BASED ON MHC-BINDING MOTIFS - COMPARISON OF PREDICTED AND PUBLISHED EPITOPES FROM MYCOBACTERIUM-TUBERCULOSIS AND HIV PROTEIN SEQUENCES SO VACCINE LA English DT Article DE MHC-BINDING; T-CELL EPITOPES; VACCINE ID CYTOTOXIC LYMPHOCYTES-T; CLASS-II MOLECULES; ALLELE-SPECIFIC MOTIFS; HEAT-SHOCK PROTEIN; IMMUNODOMINANT EPITOPE; ANCHOR RESIDUES; B-CELL; LISTERIA-MONOCYTOGENES; 65-KILODALTON PROTEIN; REVERSE-TRANSCRIPTASE AB We have designed two computer-based algorithms for T cell epitope prediction, OptiMer and EpiMer, which incorporate current knowledge of MHC-binding motifs. OptiMer locates amphipathic segments of protein antigens with a high density of MHC-binding motifs. EpiMer identifies peptides with a high density of MHC-binding motifs alone. These algorithms exploit the striking tendency for MHC-binding motifs to cluster within short segments of each protein. Putative epitopes predicted by these algorithms contain motifs corresponding to many different MHC alleles, and may contain both class I and class II motifs, features thought to be ideal for the peptide components of synthetic subunit vaccines. In this study, we describe the use of OptiMer and EpiMer for the prediction of putative T cell epitopes from Mycobacterium tuberculosis and human immunodeficiency virus protein antigens, and demonstrate that these two algorithms may provide sensitive and efficient means for the prediction of promiscuous T cell epitopes that may be critical to the development of vaccines against these and other pathogens. C1 NCI,METAB BRANCH,MOLEC IMMUNOGENET & VACCINE RES SECT,BETHESDA,MD 20892. RP MEISTER, GE (reprint author), BROWN UNIV,TB HIV RES LAB,PROVIDENCE,RI 02912, USA. OI De Groot, Annie/0000-0001-5911-1459 FU NIAID NIH HHS [R01-AI35271] NR 79 TC 115 Z9 119 U1 0 U2 2 PU BUTTERWORTH-HEINEMANN LTD PI OXFORD PA LINACRE HOUSE JORDAN HILL, OXFORD, OXON, ENGLAND OX2 8DP SN 0264-410X J9 VACCINE JI Vaccine PD APR PY 1995 VL 13 IS 6 BP 581 EP 591 DI 10.1016/0264-410X(94)00014-E PG 11 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA RB419 UT WOS:A1995RB41900009 PM 7483779 ER PT J AU LIMJOCO, T NIHRANE, A SILVER, J AF LIMJOCO, T NIHRANE, A SILVER, J TI RESISTANCE TO RETROVIRAL INFECTION IN TRANSGENIC AND BONE-MARROW CHIMERIC MICE CONTAINING FV4-ENV-EXPRESSING HEMATOPOIETIC-CELLS SO VIROLOGY LA English DT Article ID MURINE LEUKEMIA-VIRUS; FRIEND-VIRUS; ERYTHROLEUKEMIA-CELLS; ENDOPLASMIC-RETICULUM; SURFACE ANTIGENS; FV-4 RESISTANCE; DOWN-REGULATION; RFV-3 GENE; EXPRESSION; CD4 AB Mice and chickens that inherit certain retroviral envelope genes are resistant to infection with related retroviruses. Previously, we described two transgenic mouse strains bearing a retroviral envelope gene, Fv4 that confers resistance to infection with ecotropic retroviruses (T. I. Limjoco et al., 1993, 1. Virol 67, 4163-4168). Here, we present results with these and an additional transgenic strain that show that (1) the level of resistance is correlated with level of expression of the transgene, (2) low-level expression of the transgene is associated with an unexpected and possibly immune-mediated phenotype of recovery from viremia, (3) resistance can be transferred by bone marrow transplantation and is ''dominant'' in chimeras containing mixtures of transgenic resistant plus control bone marrow, and (4) transplantation after infection with Friend Virus is much less effective than transplantation before infection. We discuss the implications of these results for gene therapy of retroviral infection. C1 NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. OI Silver, Jonathan/0000-0001-9231-6368 NR 39 TC 9 Z9 9 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD APR 1 PY 1995 VL 208 IS 1 BP 75 EP 83 DI 10.1006/viro.1995.1131 PG 9 WC Virology SC Virology GA QQ934 UT WOS:A1995QQ93400009 PM 11831733 ER PT J AU KLAASSEN, VA BOESHORE, ML KOONIN, EV TIAN, TY FALK, BW AF KLAASSEN, VA BOESHORE, ML KOONIN, EV TIAN, TY FALK, BW TI GENOME STRUCTURE AND PHYLOGENETIC ANALYSIS OF LETTUCE INFECTIOUS YELLOWS VIRUS, A WHITEFLY-TRANSMITTED, BIPARTITE CLOSTEROVIRUS SO VIROLOGY LA English DT Article ID STRAND RNA VIRUSES; HEAT-SHOCK PROTEINS; NUCLEOTIDE-SEQUENCE; DNA; DOMAIN; GENE; CONSERVATION; MEMBRANE AB We report the complete nucleotide sequences of lettuce infectious yellows virus (LIYV) RNAs 1 and 2. LIYV RNA 1 is 8118 nucleotides and includes three open reading frames (ORFs). Computer-assisted analysis of LIYV RNA 1 ORFs identified domains for a papain-like protease, methyltransferase (MTR), RNA helicase (HEL), and RNA-dependent RNA polymerase (RdRp). We suggest that the RdRp domain is expressed independently of the other replication-associated domains via a +1 ribosomal frameshift. Amino acid sequences of the MTR, HEL and RdRp show highly significant similarity to the homologous sequences from other closteroviruses and tower similarity to the respective proteins of tobamoviruses, tobraviruses, hordeiviruses, bromoviruses, and furoviruses. LIYV RNA 2 is 7193 nucleotides and includes six ORFs. These ORFs include a gene array that is characteristic of the closteroviruses: ORFs encoding a small membrane protein, a homologue of the HSP70 family of chaperone proteins, a protein whose function is unknown, the coat protein, and a diverged duplicate of the coat protein. LIYV is distinguished from the monopartite closteroviruses in the following ways: its genome consists of two RNAs, the positions of the coat protein gene and its diverged duplicate are reversed, and LIYV includes ORFs that are unrelated to ORFs found in other closteroviruses. (C) 1995 Academic Press, Inc. C1 ASGROW SEED CO,KALAMAZOO,MI 49001. NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20894. RP KLAASSEN, VA (reprint author), UNIV CALIF DAVIS,DEPT PLANT PATHOL,DAVIS,CA 95616, USA. NR 45 TC 110 Z9 123 U1 0 U2 4 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD APR 1 PY 1995 VL 208 IS 1 BP 99 EP 110 DI 10.1006/viro.1995.1133 PG 12 WC Virology SC Virology GA QQ934 UT WOS:A1995QQ93400011 PM 11831736 ER PT J AU CARA, A GUARNACCIA, F REITZ, MS GALLO, RC LORI, F AF CARA, A GUARNACCIA, F REITZ, MS GALLO, RC LORI, F TI SELF-LIMITING, CELL TYPE-DEPENDENT REPLICATION OF AN INTEGRASE-DEFECTIVE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN HUMAN PRIMARY MACROPHAGES BUT NOT T-LYMPHOCYTES SO VIROLOGY LA English DT Article ID MURINE LEUKEMIA-VIRUS; PROVIRAL INTEGRATION; VIRAL-DNA; INFECTION; PROTEIN; COMPLEMENTATION; ACCUMULATION; ACTIVATION; EXPRESSION; CLEAVAGE AB Integration of retroviral DNA into the host cell genome, catalyzed by the integrase (IN) protein, is thought to be required for replication. We show here that one IN-minus defective mutant of human immunodeficiency virus type 1 (HIV-1) is able to replicate in macrophages but not in peripheral blood lymphocytes (PBLs). Replication of the HIV-1 defective mutant, however, was inefficient and self-limiting. The absence of integration in the HIV-1 IN mutant in contrast to the wild-type implies that the replication of the IN mutant depends on the transcription of the extrachromosomal forms of viral DNA. In both PBLs and macrophages circular forms of DNA were detected at significant levels, indicating that the lack of a complete functional IN protein does not preclude nuclear import of HIV-1 DNA. Cell-associated p24 was absent in the IN-defective-infected PBLs, suggesting a transcriptional block of the extrachromosomal forms of HIV-1. These results show the existence of different strategies for HIV-1. replication depending upon the cell type, and indicate the necessity of integration of viral DNA for the self-maintained progression of the infection. C1 NCI,TUMOR CELL BIOL LAB,BETHESDA,MD 20892. RI Cara, Andrea/M-4865-2015 OI Cara, Andrea/0000-0003-4967-1895 NR 29 TC 50 Z9 51 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD APR 1 PY 1995 VL 208 IS 1 BP 242 EP 248 DI 10.1006/viro.1995.1148 PG 7 WC Virology SC Virology GA QQ934 UT WOS:A1995QQ93400026 PM 11831706 ER PT J AU MORIUCHI, M MORIUCHI, H DEBRUS, S PIETTE, J COHEN, JI AF MORIUCHI, M MORIUCHI, H DEBRUS, S PIETTE, J COHEN, JI TI THE ACIDIC AMINO-TERMINAL REGION OF VARICELLA-ZOSTER VIRUS OPEN READING FRAME-4 PROTEIN IS REQUIRED FOR TRANSACTIVATION AND CAN FUNCTIONALLY REPLACE THE CORRESPONDING REGION OF HERPES-SIMPLEX VIRUS ICP27 SO VIROLOGY LA English DT Note ID COMPLETE DNA-SEQUENCE; ALPHA PROTEIN-ICP27; GENE-EXPRESSION; TRANS-REPRESSOR; TYPE-1; PROMOTERS; ACTIVATOR; HOMOLOG; MUTANTS; ACT AB Both varicella-zoster virus open reading frame 4 (ORF4) protein and its herpes simplex virus type 1 homolog ICP27 have highly acidic amino-terminal regions and cysteine-rich carboxy-terminal regions. To investigate the functional domains of these proteins, mutants were constructed and their transregulatory functions were tested in transient expression assays using two reporter plasmids, pTK-CAT-SV40A and pTK-CAT-synA, containing the same promoter sequences but different mRNA processing signals. ORF4 transactivates both pTK-CAT-SV40A and pTK-CAT-synA, while ICP27 transrepresses pTK-CAT-SV40A and transactivates pTK-CAT-synA. Deletion of the ORF4 amino-terminal region abolished most of the transactivating activity for pTK-CAT-synA but retained most of the transactivating activity for pTK-CAT-SV40A. Construction of chimeric ORF4-ICP27 molecules indicated that the ORF4 amino-terminal region was able to replace the corresponding region of ICP27 which is required for both transrepression of pTK-CAT-SV40A and transactivation of pTK-CAT-synA. Similarly, the ICP27 amino-terminal region was able to partially replace the corresponding region of ORF4 which is required for transactivation of pTK-CAT-synA. Thus, while ORF4 and ICP27 have different properties in transient expression assays, the aminoterminal regions of ORF4 and ICP27 are functionally homologous to each other and are important in regulating gene expression. (C) 1995 Academic Press, Inc. C1 NIAID,CLIN INVEST LAB,MED VIROL SECT,BETHESDA,MD 20892. UNIV LIEGE,INST PATHOL B23,DEPT MICROBIOL,FUNDAMENTAL VIROL LAB,B-4000 LIEGE,BELGIUM. NR 36 TC 16 Z9 16 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD APR 1 PY 1995 VL 208 IS 1 BP 376 EP 382 DI 10.1006/viro.1995.1164 PG 7 WC Virology SC Virology GA QQ934 UT WOS:A1995QQ93400042 PM 11831723 ER PT J AU DUFFY, CJ WURTZ, RH AF DUFFY, CJ WURTZ, RH TI MECHANISM OF THE ILLUSORY TRANSFORMATION OF OPTIC FLOW-FIELDS SO VISION RESEARCH LA English DT Letter C1 NEI,SENSORIMOTOR RES LAB,BETHESDA,MD 20892. UNIV ROCHESTER,MED CTR,DEPT NEUROL,ROCHESTER,NY 14642. UNIV ROCHESTER,MED CTR,DEPT NEUROBIOL & ANAT,ROCHESTER,NY 14642. UNIV ROCHESTER,MED CTR,DEPT OPHTHALMOL,ROCHESTER,NY 14642. UNIV ROCHESTER,MED CTR,CTR VISUAL SCI,ROCHESTER,NY 14642. NR 3 TC 2 Z9 2 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0042-6989 J9 VISION RES JI Vision Res. PD APR PY 1995 VL 35 IS 7 BP 985 EP 985 DI 10.1016/0042-6989(94)00191-N PG 1 WC Neurosciences; Ophthalmology SC Neurosciences & Neurology; Ophthalmology GA QN962 UT WOS:A1995QN96200011 ER PT J AU MCCAIN, NL CELLA, DF AF MCCAIN, NL CELLA, DF TI CORRELATES OF STRESS IN HIV DISEASE SO WESTERN JOURNAL OF NURSING RESEARCH LA English DT Article ID GAY MEN; EVENT SCALE; AIDS; INFECTION; UNCERTAINTY; NUMBER; IMMUNE; IMPACT; SEROPOSITIVITY; DEPRESSION AB A group of 53 men with HIV disease participated in this correlational study of the relationships among psychological distress, quality of life, uncertainty, coping patterns, stress, and CD4+ T-lymphocyte levels. Meaningful correlations (r > .40, p < .01) indicated that higher levels of negative-impact stressful experiences were associated with more frequent use of emotion-focused coping; both higher levels of negative stress and more frequent use of emotion-focused coping were associated with lower quality of life, higher psychological distress, and more uncertainty; lower quality of life was associated with higher psychological distress and more uncertainty; and lower CD4+ counts were associated with higher levels of positive-impact stressful experiences. C1 RUSH CANC INST,DIV PSYCHOSOCIAL ONCOL,CHICAGO,IL. RUSH PRESBYTERIAN ST LUKES MED CTR,CHICAGO,IL 60612. RUSH CANC INST,DEPT PSYCHOL & SOCIAL SCI,CHICAGO,IL. RP MCCAIN, NL (reprint author), RUSH UNIV,COLL NURSING,DEPT MED NURSING,NATL INST NURSING RES,SSH 301,1743 W HARRISON ST,CHICAGO,IL 60612, USA. FU NINR NIH HHS [T32 NR07052] NR 61 TC 29 Z9 29 U1 5 U2 5 PU SAGE PUBL INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 SN 0193-9459 J9 WESTERN J NURS RES JI West. J. Nurs. Res. PD APR PY 1995 VL 17 IS 2 BP 141 EP 155 DI 10.1177/019394599501700203 PG 15 WC Nursing SC Nursing GA RF476 UT WOS:A1995RF47600005 PM 7732682 ER PT J AU LEIDY, NK DARLINGFISHER, CS AF LEIDY, NK DARLINGFISHER, CS TI RELIABILITY AND VALIDITY OF THE MODIFIED ERIKSON PSYCHOSOCIAL STAGE INVENTORY IN DIVERSE SAMPLES SO WESTERN JOURNAL OF NURSING RESEARCH LA English DT Article ID SPAN DEVELOPMENTAL-PSYCHOLOGY; CHRONIC PHYSICAL ILLNESS; PERSONALITY-TRAITS; SOCIAL-ADJUSTMENT; HEMOPHILIC BOYS; SEX-DIFFERENCES; SELF-REPORT; INTIMACY; GENDER; ADULTHOOD AB The Modified Erikson Psychosocial Stage Inventory (MEPSI) is a relatively simple survey measure designed to assess the strength of psychosocial attributes that arise from progression through Erikson's eight stages of development. The purpose of this study was to employ secondary analysis to evaluate the internal-consistency reliability and construct validity of the MEPSI across four diverse samples: healthy young adults, hemophilic men, healthy older adults, and older adults with chronic obstructive pulmonary disease. Special attention was given to the performance of the measure across gender, with exploratory analyses examining possible age cohort and health status effects. Internal-consistency estimates for the aggregate measure were high, whereas subscale reliability levels varied across age groups. Construct validity was supported across samples. Gender, cohort, and health effects offered interesting psychometric and theoretical insights and direction for further research. Findings indicated that the MEPSI might be a useful instrument for operationalizing and testing Eriksonian developmental theory in adults. C1 UNIV MICHIGAN,SCH NURSING,ANN ARBOR,MI 48109. RP LEIDY, NK (reprint author), NINR,STUDY HUMAN RESPONSES HLTH & ILLNESS LAB,BLDG 31,ROOM 5B25,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Darling-Fisher, Cynthia/B-5796-2015 OI Darling-Fisher, Cynthia/0000-0002-2145-4875 FU BHP HRSA HHS [F31-NU05685, F31-NU05758] NR 73 TC 6 Z9 6 U1 2 U2 4 PU SAGE PUBL INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 SN 0193-9459 J9 WESTERN J NURS RES JI West. J. Nurs. Res. PD APR PY 1995 VL 17 IS 2 BP 168 EP 187 DI 10.1177/019394599501700205 PG 20 WC Nursing SC Nursing GA RF476 UT WOS:A1995RF47600007 PM 7732684 ER PT J AU EILS, R SARACOGLU, K MUNKEL, C IMHOFF, J SATZLER, K BERTIN, E DIETZEL, S SCHROCK, E RIED, T CREMER, T CREMER, C AF EILS, R SARACOGLU, K MUNKEL, C IMHOFF, J SATZLER, K BERTIN, E DIETZEL, S SCHROCK, E RIED, T CREMER, T CREMER, C TI 3-DIMENSIONAL IMAGING APPROACHES AND MONTE-CARLO SIMULATIONS - DEVELOPMENT OF TOOLS TO STUDY THE MORPHOLOGY AND DISTRIBUTION OF CHROMOSOME TERRITORIES AND SUBCHROMOSOMAL TARGETS IN HUMAN CELL-NUCLEI SO ZOOLOGICAL STUDIES LA English DT Article; Proceedings Paper CT Focus on Microscopy 95 Conference CY APR 18-20, 1995 CL TAIPEI, TAIWAN SP ACADEMIA SINICA, ROC, ACADEMIA SINICA, INST ZOOL, NANKANG, LIFE SCI RES PROMOT CTR NSC, ROC, ELECTRON MICROSCOPY SOC CHINA, TAIPEI, ROC, SOC 3 D IMAGING SCI MICROSCOPY, AMSTERDAM, SUNY, AMIL, BUFFALO, NY C1 UNIV HEIDELBERG,INST PHYS APPL,D-69115 HEIDELBERG,GERMANY. UNIV HEIDELBERG,INST THEORET PHYS,D-69115 HEIDELBERG,GERMANY. UNIV GRENOBLE 1,EQUIPE DYOGEN & LAB TIMC,CNRS,URA 1618,GRENOBLE,FRANCE. UNIV HEIDELBERG,INST HUMAN GENET & ANTHROPOL,D-69120 HEIDELBERG,GERMANY. NIH,NATL CTR HUMAN GENOME RES,BETHESDA,MD 20892. RP EILS, R (reprint author), UNIV HEIDELBERG,DISCIPLINARY CTR SCI COMP IWR 1,GRADUIERTENKOLLEG MODELING & SCI COMP MATH & SCI,D-69120 HEIDELBERG,GERMANY. RI Satzler, Kurt/E-9910-2012; Eils, Roland/B-6121-2009 OI Eils, Roland/0000-0002-0034-4036 NR 6 TC 7 Z9 7 U1 0 U2 2 PU ACAD SINICA INST ZOOLOGY PI TAIWAN 115 PA EDITORIAL OFFICE TAIPEI, TAIWAN 115, REP OF CHINA SN 1021-5506 J9 ZOOL STUD JI Zool. Stud. PD APR PY 1995 VL 34 SU 1 BP 7 EP 10 PG 4 WC Zoology SC Zoology GA RA786 UT WOS:A1995RA78600003 ER PT J AU JUNG, K KWON, M LEE, HY LEE, HW HONG, S AF JUNG, K KWON, M LEE, HY LEE, HW HONG, S TI PURIFICATION AND CHARACTERIZATION OF PROTEIN METHYLASE-II FROM PORCINE TESTIS SO JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE PORCINE TESTIS; PROTEIN METHYLASE II ID CARBOXYL METHYLTRANSFERASE; SIGNAL TRANSDUCTION; BOVINE BRAIN; WHEAT-GERM; CARBOXYMETHYLASE; ISOZYMES AB Protein methylase II (S-adenosyl-L-methionine : protein O-methyl-transferase, EC 2.1.1.24; PM II) was purified approximately 1250-fold from porcine testis by fractional precipitation and DEAE-cellulose chromatography, followed by gel filtration on a Sephadex G-75 column and HPLC on a Protein Pak 125 column. The molecular weight of the enzyme was estimated to be 33,000 daltons by SDS-PAGE, which agreed with the value determined by gel filtration. Isoelectric focusing of purified PM II showed a single protein species with an isoelectric point of 6.2. The optimum pH for the reaction was 6.0. The K-m value of the enzyme was 1X10(-5) M with a V-max value of 769 pmol/min/mg of enzyme. S-adenosyl-L-homosysteine is a competitive inhibitor of PM II with a K-i value of 1.38X10(-6) M. C1 NCI, PATHOL LAB, BETHESDA, MD 20892 USA. SUNGKYUNKWAN UNIV, COLL PHARM, BIOCHEM LAB, SUWON 440746, SOUTH KOREA. SUNGKYUNKWAN UNIV, COLL LIFE SCI & NAT RESOURCES, DEPT GENET ENGN, SUWON 440746, SOUTH KOREA. NR 30 TC 3 Z9 3 U1 0 U2 0 PU SPRINGER SINGAPORE PTE LTD PI SINGAPORE PA #04-01 CENCON I, 1 TANNERY RD, SINGAPORE 347719, SINGAPORE SN 1225-8687 J9 J BIOCHEM MOL BIOL JI J. Biochem. Mol. Biol. PD MAR 31 PY 1995 VL 28 IS 2 BP 149 EP 154 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QW387 UT WOS:A1995QW38700011 ER PT J AU YU, JC LI, WQ WANG, LM UREN, A PIERCE, JH HEIDARAN, MA AF YU, JC LI, WQ WANG, LM UREN, A PIERCE, JH HEIDARAN, MA TI DIFFERENTIAL REQUIREMENT OF A MOTIF WITHIN THE CARBOXYLTERMINAL DOMAIN OF ALPHA-PLATELET-DERIVED GROWTH-FACTOR (ALPHA-PDGF) RECEPTOR FOR PDGF FOCUS-FORMING ACTIVITY CHEMOTAXIS, OR GROWTH SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Note ID KINASE INSERT DOMAIN; SIGNALING PATHWAYS; PHOSPHOLIPASE-C; EXPRESSION; SEQUENCE; PROTEIN AB To determine the molecular basis for the transforming function of platelet derived growth factor (PDGF)-A in NIH/3T3 cells, we have constructed chimerae consisting of the extracellular domain of the human CSF-1R (fms) linked to the cytoplasmic domain of the alpha PDGF receptor (alpha R) containing a series of deletion or point mutations. The ability of fms/alpha R chimerae to mediate CSF-l-dependent anchorage-independent growth, focus formation, and chemotaxis of NIH/3T3 cells was then examined. Our results provide evidence that a domain encompassing amino acid residues 977-1024 of the alpha PDGFR is required for ligand-dependent focus formation, but not chemotaxis or anchorage-independent growth, and that tyrosine residues within this domain constitute the major binding site for phospholipase C gamma. Therefore, our findings suggest that: (i) the focus forming function of alpha PDGFR correlates well with the ability of the receptor to bind phospholipase C gamma, and (ii) the mechanism of focus formation mediated by (alpha PDGFR may be distinguished from that required for chemotaxis or anchorage-independent growth. C1 NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892. NR 23 TC 22 Z9 22 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 31 PY 1995 VL 270 IS 13 BP 7033 EP 7036 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ431 UT WOS:A1995QQ43100006 PM 7706238 ER PT J AU GHOSH, M HOWARD, KJ CAMERON, CE BENKOVIC, SJ HUGHES, SH LEGRICE, SFJ AF GHOSH, M HOWARD, KJ CAMERON, CE BENKOVIC, SJ HUGHES, SH LEGRICE, SFJ TI TRUNCATING ALPHA-HELIX E' OF P66 HUMAN-IMMUNODEFICIENCY-VIRUS REVERSE-TRANSCRIPTASE MODULATES RNASE-H FUNCTION AND IMPAIRS DNA STRAND TRANSFER SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HIV-1 RIBONUCLEASE-H; AMINO-ACID-RESIDUES; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; ANGSTROM RESOLUTION; MUTATIONAL ANALYSIS; DOMAIN; POLYMERASE; MUTAGENESIS; INHIBITOR AB The properties of recombinant p66/p51 human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) containing C-terminal truncations in its p66 polypeptide were evaluated, Deletion end points partly or completely removed alpha-helix E' of the RNase H domain (p66 Delta 8/p51 and p66 Delta 16/p51, respectively), while mutant p66 Delta 23/p51 lacked alpha E' and the beta 5'-alpha E' connecting loop. Although dimerization and DNA polymerase properties of all mutants were not significantly different from those of the parental enzyme, p66 Delta 16/p51 and p66 Delta 23/p51 RT lacked ribonuclease H (RNase H) activity, In contrast, RT mutant p66 Delta 8/p51 retained endonuclease activity but lacked the directional processing feature of the parental enzyme, Despite retaining full endoribonuclease function, p66 Delta 8/p51 RT barely supported transfer of nascent (-)-strand DNA between RNA templates representing the 5' and 3' ends of retroviral genome, shedding light on the requirement for the endonuclease and directional processing functions of the RNase H domain during replication. C1 CASE WESTERN RESERVE UNIV,SCH MED,DIV INFECT DIS,CLEVELAND,OH 44106. PENN STATE UNIV,DEPT CHEM,UNIVERSITY PK,PA 16802. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21702. FU NCI NIH HHS [N01-CO-74101]; NIGMS NIH HHS [GM46623, GM13306] NR 47 TC 48 Z9 48 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 31 PY 1995 VL 270 IS 13 BP 7068 EP 7076 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ431 UT WOS:A1995QQ43100013 PM 7535765 ER PT J AU SCHULTZCHERRY, S CHEN, H MOSHER, DF MISENHEIMER, TM KRUTZSCH, HC ROBERTS, DD MURPHYULLRICH, JE AF SCHULTZCHERRY, S CHEN, H MOSHER, DF MISENHEIMER, TM KRUTZSCH, HC ROBERTS, DD MURPHYULLRICH, JE TI REGULATION OF TRANSFORMING GROWTH-FACTOR-BETA ACTIVATION BY DISCRETE SEQUENCES OF THROMBOSPONDIN-1 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HEPARIN-BINDING PEPTIDES; MELANOMA CELL-ADHESION; I REPEATS; CALCINEURIN-A; IDENTIFICATION; FIBRONECTIN; PHYSIOLOGY; EXPRESSION; PROPERDIN; PROTEINS AB Transforming growth factor-beta (TGF-beta) is a potent growth regulatory protein secreted by virtually all cells in a latent form. A major mechanism of regulating TGF-beta activity occurs through factors that central the process ing of the latent to the biologically active form of the molecule. We have shown previously that thrombospondin 1 (TSP1), a platelet alpha-granule and extracellular matrix protein, activates latent TGF-beta via a protease and cell-independent mechanism and have localized the TGF-beta binding/activation region to the type 1 repeats of platelet TSP1. We now report that recombinant human TSP1, but not recombinant mouse TSP2, activates latent TGF-beta. Activation was further localized to the unique sequence RFK found between the first and the second type 1 repeats of TSP1 (amino acids 412-415) by the use of synthetic peptides. A peptide with the corresponding sequence in TSP2, RLR, was inactive. In addition, a hexapeptide GGWSHW, based on a sequence present in the type 1 repeats of both TSP1 and TSP2, inhibited the activation of latent TGF-beta by TSP1. This peptide bound to I-125-active TGF-beta and inhibited interactions of TSP1 with latent TGF-beta. TSP2 also inhibited activation of latent TGF-beta by TSP1, presumably by competitively binding to TGF-beta through the WSHW sequence. These studies show that activation of latent TGF-beta is mediated by two sequences present in the type 1 repeats of TSP1, a sequence (GGWSHW) that binds active TGF-beta and potentially orients the TSP molecule and a second sequence (RFK) that activates latent TGF-beta. Peptides based on these sites have potential therapeutic applications for modulation of TGF-beta activation. C1 UNIV ALABAMA,DEPT PATHOL,DIV MOLEC & CELLULAR PATHOL,BIRMINGHAM,AL 35294. UNIV WISCONSIN,DEPT BIOMOLEC CHEM,MADISON,WI 53706. UNIV WISCONSIN,DEPT MED,MADISON,WI 53706. NCI,PATHOL LAB,BETHESDA,MD 20892. RI Roberts, David/A-9699-2008 OI Roberts, David/0000-0002-2481-2981 FU NHLBI NIH HHS [HL08640, HL49111, HL50061, R01 HL050061] NR 36 TC 292 Z9 299 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 31 PY 1995 VL 270 IS 13 BP 7304 EP 7310 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ431 UT WOS:A1995QQ43100044 PM 7706271 ER PT J AU FIERER, DS CHALLBERG, MD AF FIERER, DS CHALLBERG, MD TI THE STOICHIOMETRY OF BINDING OF THE HERPES-SIMPLEX VIRUS TYPE-1 ORIGIN-BINDING PROTEIN, UL9, TO ORI(S) SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID VIRAL-DNA REPLICATION; ESCHERICHIA-COLI; GEL RETARDATION; LAC PROMOTER; DOMAIN; ORIS; SEQUENCE; HELICASE; GENE; PURIFICATION AB A number of studies have demonstrated that the herpes simplex virus type 1 (HSV-1) UL9 protein, which is a homodimer in solution, binds to two high affinity binding sites in each origin of replication. Interaction between the proteins bound at the two sites leads to the formation of a complex nucleoprotein structure. The simplest models for this binding interaction predict two possible binding stoichiometries: 1) one UL9 dimer is bound at each site; or 2) one UL9 monomer is bound at each site so that one UL9 dimer occupies both sites, Two recent papers have addressed this issue by using indirect methods to measure the binding stoichiometry, Martin et al. (Martin, D. W., Munoz, R. M., Oliver, D., Subler, M. A., and Deb, S. (1994) Virology 198, 71-80) reported that a monomer of UL9 binds to a single high affinity site, and Stabell and Olive (Stabell, E. C., and Olive, P. D. (1993) Nucleic Acids Res. 21, 5203-5211) concluded that a dimer of UL9 binds to a single high affinity site. We have directly measured the stoichiometry of binding of the carboxyl terminal DNA binding domain of UL9 (t-UL9) to the origin of replication using a double-label gel shift assay. Using a short synthetic double-stranded oligonucleotide containing a single UL9 binding site, one protein-DNA complex was detected in the gel shift assay, and the molar ratio of UL9 DNA binding domains to DNA binding sites in this complex was determined to be 2.0 +/- 0.1 (n = 13). Using the minimal origin sequence excised from plasmid DNA, two protein-DNA complexes were detected, The binding stoichiometry of the faster migrating complex was 1.8 +/- 0.1 (n = 15), and the stoichiometry of the more slowly migrating band was 3.7 +/- 0.4 (n = 15), The simplest explanation for these data is that UL9 binds to the origin of replication as a homodimer with one dimer bound at both high affinity sites. C1 NIAID,VIRAL DIS LAB,BETHESDA,MD 20892. NR 40 TC 20 Z9 20 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 31 PY 1995 VL 270 IS 13 BP 7330 EP 7334 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ431 UT WOS:A1995QQ43100047 PM 7706274 ER PT J AU BROWN, JA BHARATHI, A GHOSH, A WHALEN, W FITZGERALD, E DHAR, R AF BROWN, JA BHARATHI, A GHOSH, A WHALEN, W FITZGERALD, E DHAR, R TI A MUTATION IN THE SCHIZOSACCHAROMYCES-POMBE RAE1 GENE CAUSES DEFECTS IN POLY(A)(+) RNA EXPORT AND IN THE CYTOSKELETON SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MESSENGER-RNA; FISSION YEAST; SACCHAROMYCES-CEREVISIAE; CHROMOSOME CONDENSATION; MICROTUBULE FUNCTION; BETA-SUBUNITS; PROTEIN; TRANSPORT; MUTANT; INITIATION AB A collection of fission yeast Schizosaccharomyces pombe conditional mutants was screened for defective nucleocytoplasmic transport of poly(A)(+) RNA by fluorescence in situ hybridization, We identified a temperature-sensitive mutant that accumulated poly(A)(+) RNA in the nucleus and have named it rae1-1, for ribonucleic acid export. All rae1-1 cells exhibit the defect in poly(A)(+) RNA export within 30 min following a shift to the nonpermissive temperature, In addition, in the rae1-1 mutant, actin and tubulin become disorganized, and cells undergo an irreversible cycle arrest, Results from experiments in which rae1-1 cells were arrested in various phases of the cell division cycle and then shifted to nonpermissive temperature suggest that cells are particularly vulnerable to loss of rae1 function during G(2)/M. However, the inability to export RNA from the nucleus to the cytoplasm was not limited to a particular phase of the cell division cycle, The rae1 gene was isolated by complementation and encodes a predicted protein of 352 amino acids with four beta-transducin/WD40 repeats. C1 NCI, MOLEC VIROL LAB, BETHESDA, MD 20892 USA. NCI, SURG BRANCH, BETHESDA, MD 20892 USA. NR 42 TC 113 Z9 116 U1 1 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 31 PY 1995 VL 270 IS 13 BP 7411 EP 7419 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ431 UT WOS:A1995QQ43100060 PM 7706287 ER PT J AU TSAIMORRIS, CH GENG, Y BUCZKO, E DUFAU, ML AF TSAIMORRIS, CH GENG, Y BUCZKO, E DUFAU, ML TI CHARACTERIZATION OF DIVERSE FUNCTIONAL ELEMENTS IN THE UPSTREAM SP1 DOMAIN OF THE RAT LUTEINIZING-HORMONE RECEPTOR GENE PROMOTER SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID TRANSCRIPTION; DNA; BOX; RECOGNITION; ACTIVATION; SEQUENCE; PROTEINS; CLONING; BINDS; SITES AB Transcription of the luteinizing hormone receptor gene is dependent on Sp1-induced promoter activation from two Sp1 binding domains (Sp1(2) and Sp1(4)) within the 173-base pair promoter. Of the two Sp1 binding domains, the canonical GC box (GGGCGG) was determined by mutation to be the binding element for only the Sp1(2) domain. The Sp1 binding element within the Sp1(4) domain was identified by mutation and immunological/competition studies as the 5'-GGG GTG GGG that conforms to a Zif-268 like three zinc finger binding domain, rather than the canonical 3' Sp1(4) GC box (GGGCGG). The guanines in the third trinucleotide (GGG GTG GGG) were not required for Sp1 binding, although they increased binding affinity, Non-Sp1 protein(s) bind the 3' Sp1(4), GC box, and by themselves exhibit transcriptional activity, Tissue specific differences were localized to this non-Sp1 binding domain, which functionally substituted for the downstream activating M1 regulatory domain in non expressing but not in expressing cells, Mutations of both non-Sp1 and M1 domains were required for inhibition of promoter activity in constructs that retained the Sp1 binding elements in non expressing cells, indicating that together these domains may play a role in regulation of luteinizing hormone receptor gene expression. C1 NICHHD,ENDOCRINOL & REPROD RES BRANCH,MOLEC ENDOCRINOL SECT,BETHESDA,MD 20892. NR 27 TC 26 Z9 26 U1 1 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 31 PY 1995 VL 270 IS 13 BP 7487 EP 7494 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ431 UT WOS:A1995QQ43100070 PM 7706295 ER PT J AU GENTRYWEEKS, CR SPOKES, J THOMPSON, J AF GENTRYWEEKS, CR SPOKES, J THOMPSON, J TI BETA-CYSTATHIONASE FROM BORDETELLA-AVIUM - ROLE(S) OF LYSINE-214 AND CYSTEINE RESIDUES IN ACTIVITY AND CYTOTOXICITY SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ALANINE-SCANNING MUTAGENESIS; PYRIDOXAL-PHOSPHATE; BINDING-SITE; TRYPTOPHAN SYNTHASE; ESCHERICHIA-COLI; SULFANE SULFUR; SUBSTITUTION; POLYMERASE; SUBUNIT; GENES AB beta-Cystathionase (EC 4.4.1.8) from Bordetella avium is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the hydrolysis of L-cystine to yield pyruvic acid, NH3, and thiocysteine. The latter compound is highly toxic toward MC3T3-E1 osteogenic cells, rat os teosarcoma cells, and other cell lines maintained in tissue culture (Gentry-Weeks, C. R., Keith, J. M., and Thompson, J. (1993) J. Biol. Chem. 268, 7298-7314). Site directed mutagenesis has established that lysine 214 of the sequence TKYVGGHSD, is primarily responsible for internal aldimine binding of PLP in the holoenzyme. Translation of the DNA sequence of the beta-cystathionase gene (metC) from B. avium, reveals 4 cysteine residues/enzyme subunit (M(r) = 42,600), and spectrophotometric analysis with 4,4' dithiodipyridine showed that there were no disulfide linkages in the native protein. beta-Cystathionase is inhibited by sulfhydryl-reactive agents, including N-ethyhmaleimide (NEM). To elucidate the mechanism of NEM inhibition, each of the 4 cysteine residues at positions 88, 117, 279, and 309 was individually replaced by alanine or glycine. The mutant proteins C88A, C117G, C279G, and C309A were purified to homogeneity, and each was assayed for enzyme activity, PLP-binding, NEM sensitivity, and susceptibility to chymotrypsin digestion. The activities of mutant proteins C88A and C279G were comparable with that of the native enzyme, and since both forms were inhibited by NEM, neither cysteine 88 nor 279 are prerequisite for enzyme activity. By elimination, cysteine residues 117 and 309 must be the targets for alkylation, and resultant inactivation of beta-cystathionase, by the -SH reactive agent. Substitution of cysteine 117 and 309 with glycine and alanine, respectively, yielded the inactive proteins C117G and C309A. PLP was not detectable in these proteins, and their absorption spectra lacked the peak (at 420 nm) that is characteristic of internal PLP-Schiff base formation. Edman degradation revealed that C117G (M(r) similar to 36,000) also lacked the first 63 amino acids comprising the N terminus of the native protein. The beta-cystathionase mutants C117G and C309A showed enhanced susceptibility to chymotrypsin digestion. Cysteine residues 117 and 309 may reside in conformationally sensitive environments, and in the native enzyme these amino acids most probably serve a structural function. Toxicity assays performed with the various mutant proteins obtained by site-directed mutagenesis established that only catalytically active forms of beta-cystathionase were cytotoxic for tissue culture cells. RP GENTRYWEEKS, CR (reprint author), NIDR,MICROBIAL ECOL LAB,BLDG 30,RM 532,30 CONVENT DR,MSC 4350,BETHESDA,MD 20892, USA. NR 31 TC 9 Z9 9 U1 0 U2 7 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 31 PY 1995 VL 270 IS 13 BP 7695 EP 7702 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ431 UT WOS:A1995QQ43100096 PM 7706318 ER PT J AU BAI, G KUSIAK, JW AF BAI, G KUSIAK, JW TI FUNCTIONAL-ANALYSIS OF THE PROXIMAL 5'-FLANKING REGION OF THE N-METHYL-D-ASPARTATE RECEPTOR SUBUNIT GENE, NMDAR1 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID TRANSCRIPTION FACTOR; MESSENGER-RNA; BINDING PROTEIN; NERVOUS-SYSTEM; GABA-A; PROMOTER; RAT; EXPRESSION; CELLS; CLONING AB The NMDAR1 receptor subunit is a common subunit of N-methyl-D-aspartate receptors. We have previously characterized 3 kilobases (kb) of 5'-flanking sequence of the NMDAR1 gene and now report on the ability of this region to direct transcription of a reporter gene and on its interaction with nuclear proteins, The sequence 356 base pairs (bp) 5' of the first nucleotide of codon 1 was sufficient to express a luciferase reporter gene in rat PC12 pheochromocytoma cells, Additional sequences upstream of nucleotide -356 influenced the activity approximately 2-fold. A labeled 112-bp fragment (position -356 to -245) formed six complexes (C1A and -B, C2A and -B, and C3A and -B), grouped as three double bands, with nuclear extracts from PC12 cells, Competition with Sp1 oligonucleotides abolished formation of C2A and -B and C3A and -B complexes. Sp1 antibody recognized the C3A complex in supershift experiments, Prior immunoprecipitation of nuclear extracts with Spl antibody abolished formation of C2A and -B and C3A and -B complexes. Purified Sp1 protein alone did not form a C3A complex but potentiated its formation when PC12 nuclear extract was added, A CC-rich sequence in this fragment was protected from DNase I digestion by nuclear extract, These results suggest that a 356-bp sequence comprises the NMDAR1 basal promoter, and that NMDAR1 gene expression may be regulated by Sp1-like nuclear factors. RP BAI, G (reprint author), NIA,GERONTOL RES CTR,MOLEC NEUROBIOL UNIT,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 55 TC 72 Z9 73 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 31 PY 1995 VL 270 IS 13 BP 7737 EP 7744 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ431 UT WOS:A1995QQ43100102 PM 7706322 ER PT J AU SIDDIQI, SM JACOBSON, KA ESKER, JL OLAH, ME JI, XD MELMAN, N TIWARI, KN SECRIST, JA SCHNELLER, SW CRISTALLI, G STILES, GL JOHNSON, CR IJZERMAN, AP AF SIDDIQI, SM JACOBSON, KA ESKER, JL OLAH, ME JI, XD MELMAN, N TIWARI, KN SECRIST, JA SCHNELLER, SW CRISTALLI, G STILES, GL JOHNSON, CR IJZERMAN, AP TI SEARCH FOR NEW PURINE-MODIFIED AND RIBOSE-MODIFIED ADENOSINE-ANALOGS AS SELECTIVE AGONISTS AND ANTAGONISTS AT ADENOSINE RECEPTORS SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID CARBOCYCLIC ANALOGS; RAT-BRAIN; NUCLEOSIDES; DERIVATIVES; ARISTEROMYCIN; ENANTIOMERS; RESOLUTION AB The binding affinities at rat A(1), A(2a), and A(3) adenosine receptors of a wide range of derivatives of adenosine have been determined. Sites of modification include the purine moiety (1-, 3-, and 7-deaza; halo, alkyne, and amino substitutions at the 2- and 8-positions; and N6(-)CH(2)-ring, -hydrazino, and -hydroxylamino) and the ribose moiety (2'-, 3'-, and 5'-deoxy; 2'- and 3'-O-methyl;2'-deoxy 2'-fluoro;6'-thio;5'-uronamide;carbocyclic;4'- or 3'-methyl; and inversion of configuration. (-)- and (+)-5'-Noraristeromycin were 48- and 21-fold selective, respectively, for A(2a), vs A(1) receptors. 2-Chloro-6'-thioadenosine displayed a K-i value of 20 nM at A(2a) receptors (15-fold selective vs A(1)). 2-Chloroadenin-9-yl(beta-L-2'-deoxy-6'-thiolyxofuranoside) displayed a K-i value of 8 mu M at A(1) receptors and appeared to be an antagonist, on the basis of the absence of a GTP-induced shift in binding vs a radiolabeled antagonist (8-cyclopentyl-1,3-dipropylxanthine). 2-Chloro-2'-deoxyadenosine and 2-chloroadenin-9-yl(beta-D-6'-thioarabinoside) were putative partial agonists at A(1) receptors, with K-i values of 7.4 and 5.4 mu M, respectively. The A(2a) selective agonist 2-(1-hexynyl)-5'-(N-ethylcarbamoyl)adenosine displayed a K-i value of 26 nM at A(3) receptors. The 4'-methyl substitution of adenosine was poorly tolerated, yet when combined with other favorable modifications, potency was restored. Thus, N-6-benzyl-4'methyladenosine-5'-(N-methyluronamide) displayed a K-i value of 604 nM at A(3) receptors and was 103- and 88-fold selective vs A(1) and A(2a) receptors, respectively. This compound was a full agonist in the A(3)-mediated inhibition of adenylate cyclase in transfected CHO cells. The carbocyclic analogue of N-6-(3-iodobenzyl)adenosine-5'-(N-methyluronamide) was 2-fold selective for A(3) VS A(1) receptors and was nearly inactive at A(2a) receptors. C1 NIDDK, LBC, MRS, BETHESDA, MD 20892 USA. SO RES INST, ORGAN CHEM RES DEPT, BIRMINGHAM, AL 35255 USA. WAYNE STATE UNIV, DEPT CHEM, DETROIT, MI 48202 USA. DUKE UNIV, MED CTR, DEPT MED, DURHAM, NC 27710 USA. UNIV CAMERINO, DIPARTIMENTO SCI CHIM, I-62032 CAMERINO, ITALY. LEIDEN AMSTERDAM CTR DRUG RES, DIV MED CHEM, 2300 RA LEIDEN, NETHERLANDS. AUBURN UNIV, DEPT CHEM, AUBURN, AL 36849 USA. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z01 DK031117-20, Z99 DK999999]; NIAID NIH HHS [N01-AI-72645, R01 AI072645] NR 65 TC 81 Z9 82 U1 0 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0022-2623 EI 1520-4804 J9 J MED CHEM JI J. Med. Chem. PD MAR 31 PY 1995 VL 38 IS 7 BP 1174 EP 1188 DI 10.1021/jm00007a014 PG 15 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA QQ921 UT WOS:A1995QQ92100014 PM 7707320 ER PT J AU FORD, H SIDDIQUI, MA DRISCOLL, JS MARQUEZ, VE KELLEY, JA MITSUYA, H SHIRASAKA, T AF FORD, H SIDDIQUI, MA DRISCOLL, JS MARQUEZ, VE KELLEY, JA MITSUYA, H SHIRASAKA, T TI LIPOPHILIC, ACID-STABLE, ADENOSINE DEAMINASE-ACTIVATED ANTI-HIV PRODRUGS FOR CENTRAL-NERVOUS-SYSTEM DELIVERY .2. 6-HALO AND 6-ALKOXY PRODRUGS OF 2'-BETA-FLUORO-2',3'-DIDEOXYINOSINE SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID BLOOD-BRAIN-BARRIER; CEREBROSPINAL-FLUID; 2',3'-DIDEOXYPURINE NUCLEOSIDES; TUBERCULOUS MENINGITIS; CATALYZED HYDROLYSIS; ADENINE NUCLEOSIDES; HTLV-III; INFECTION; AIDS; 2',3'-DIDEOXYADENOSINE AB A series of 6-halo-(F-, Cl-, Br-, I-) and 6-alkoxy-(OMe-, OEt-) 9-(2,3-dideoxy-2-fluoro-beta-D-threo-pentofuranosyl) purines (F-ddN) have been synthesized and characterized with the objective of finding compounds which might be superior to existing drugs for the treatment of HIV in the central nervous system. These compounds, which contain lipophilic 6-substituents, were chosen as acid-stable prodrugs for the anti-HIV-active F-ddN, 9-(2,3-dideoxy-2-fluoro-beta-D-threo-pentofuranosyl) hypoxanthine (F-ddI), because of their potential to increase blood-brain-barrier penetration relative to F-ddI. All the new compounds were more lipophilic than the currently approved anti-AIDS drugs. Partition coefficient increases of 30- and 110-fold were achieved, relative to didanosine (ddI), for the 6-chloro- and 6-ethoxy analogues; 2'-Fluoro substitution abolished the pH 1, acid-catalyzed cleavage of the nucleoside glycosylic bond. However, pH 1, acid-catalyzed hydrolysis of the 6-fluoro substituent to produce: F-ddI was observed to occur at a rate (t(1/2) 0.54 h) which was ca. 40-170 times faster than that of the other prodrugs. The utility of the F-dCLNs as prodrugs for F-ddI depends upon their ability to act as substrates for. adenosine deaminase. The relative rates of adenosine deaminase-catalyzed prodrug hydrolysis to F-ddI varied by a factor of >25 000 with the 6-fluoro- and 6-ethoxy analogues reacting the fastest and slowest, respectively. All of the prodrugs possessed anti-HIV activity in the phytohemagglutinin-stimulated peripheral blood mononuclear cell test system and a qualitative correlation exists between prodrug anti-HIV activity and adenosine deaminase hydrolysis rates. C1 NCI,MED CHEM LAB,DEV THERAPEUT PROGRAM,BETHESDA,MD 20892. NCI,DIV CANC TREATMENT,CLIN ONCOL PROGRAM,MED BRANCH,EXPTL RETROVIROL SECT,BETHESDA,MD 20892. NR 53 TC 30 Z9 31 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA PO BOX 57136, WASHINGTON, DC 20037-0136 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD MAR 31 PY 1995 VL 38 IS 7 BP 1189 EP 1195 DI 10.1021/jm00007a015 PG 7 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA QQ921 UT WOS:A1995QQ92100015 PM 7707321 ER PT J AU MOORE, SA SIELECKI, AR CHERNAIA, MM TARASOVA, NI JAMES, MNG AF MOORE, SA SIELECKI, AR CHERNAIA, MM TARASOVA, NI JAMES, MNG TI CRYSTAL AND MOLECULAR-STRUCTURES OF HUMAN PROGASTRICSIN AT 1.62 ANGSTROM RESOLUTION SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE PROGASTRICSIN; ZYMOGEN; ASPARTIC PROTEINASE; CRYSTALLOGRAPHY ID AMINO-ACID-SEQUENCE; PEPSINOGEN-C PROGASTRICSIN; MULTIWIRE AREA DETECTOR; PORCINE PEPSINOGEN; GASTRIC-MUCOSA; MACROMOLECULAR STRUCTURES; ASPARTIC PROTEINASES; TRANSLATION-FUNCTION; GENE DUPLICATION; 1.8-A RESOLUTION AB The crystal and molecular structures of human progastricsin (hPGC) have been determined using multiple isomorphous replacement methods and anomalous scattering in conjunction with a phased translation function. The structure has been refined to a conventional R-factor (=Sigma parallel to F-o\ - \F-c parallel to/Sigma\F-o\) of 0.179 with data to 1.62 Angstrom resolution. The first 37 amino acid residues of the prosegment are similar in conformation to the equivalent residues of porcine pepsinogen (pPGN). As in pPGN, the N-zeta atom of Lys37p sits between the active-site carboxylate groups of Asp32 and Asp217, thereby preventing catalysis. The side-chains of Tyr38p and Tyr9 sit in the S1' and S1 substrate-binding pockets of hPGC, respectively, in an analogous manner to what is observed in porcine pepsinogen. There are large conformational differences centered around the region containing residues Arg39p to Pro6, relative to the equivalent region in the structure of pPGN. Two surface loops in the vicinity of this segment are also displaced relative to those in pPGN and in mature aspartic proteinases (Phe71 to Thr81 (the ''flap''), and Tyr125 to Thr131). In hPGC, Tyr75 O-eta does not make its usual hydrogen bond to Trp39 N-epsilon l. Rather, the ''flap'' containing Tyr75 is excluded from the active site by the polypeptide segment Arg39p to Pro6. However, the conformation of the inhibitory segment, Lys37p to Tyr38p, is virtually identical with that observed in pPGN. Hence the structures of these two proteins indicate that aspartic proteinase zymogens keep themselves inactive at neutral pH by a very similar mechanism in human progastricsin and porcine pepsinogen. This similarity likely carries over to all members of both the pepsinogen A and C families of aspartic proteinase zymogens. C1 UNIV ALBERTA,DEPT BIOCHEM,CANADA GRP PROT STUCT & FUNCT,MRC,EDMONTON,AB T6G 2H7,CANADA. RUSSIAN ACAD SCI,VA ENGELHARDT MOLEC BIOL INST,MOSCOW,RUSSIA. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MOLEC ASPECTS DRUG DESIGN SECT,FREDERICK,MD 21702. FU NCI NIH HHS [N01-CO-74101] NR 75 TC 53 Z9 56 U1 0 U2 0 PU ACADEMIC PRESS (LONDON) LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD MAR 31 PY 1995 VL 247 IS 3 BP 466 EP 485 DI 10.1006/jmbi.1994.0154 PG 20 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ236 UT WOS:A1995QQ23600009 PM 7714902 ER PT J AU VARMUS, H AF VARMUS, H TI NIH REVIEW OF GENE-THERAPY PROTOCOLS SO SCIENCE LA English DT Letter RP VARMUS, H (reprint author), NIH,BLDG 10,BETHESDA,MD 20892, USA. NR 2 TC 4 Z9 4 U1 1 U2 1 PU AMER ASSOC ADVAN SCIENCE PI WASHINGTON PA 1333 H ST NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 31 PY 1995 VL 267 IS 5206 BP 1889 EP 1889 DI 10.1126/science.7701310 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QQ068 UT WOS:A1995QQ06800002 PM 7701310 ER PT J AU ZEROMSKI, J JEZEWSKA, E SIKORA, J KASPRZAK, KS AF ZEROMSKI, J JEZEWSKA, E SIKORA, J KASPRZAK, KS TI THE EFFECT OF NICKEL COMPOUNDS ON IMMUNOPHENOTYPE AND NATURAL-KILLER-CELL FUNCTION OF NORMAL HUMAN-LYMPHOCYTES SO TOXICOLOGY LA English DT Article DE NICKEL SALTS; NICKEL IMMUNOTOXICITY; HUMAN IMMUNE CELLS; IMMUNOPHENOTYPE; NATURAL KILLER CELLS ID MAGNESIUM; IMMUNITY; CHLORIDE AB In order to elucidate effects of nickel on human lymphocytes in vitro, peripheral blood mononuclear cells from normal donors were initially tested for viability in the presence of increasing concentrations of two selected nickel salts, sparingly-soluble nickel subsulfide (Ni3S2), and promptly-soluble nicker sulfate (NiSO4). After establishing the toxicity profile, the cells were cultured for 24 h with each compound at three nontoxic concentrations, 0.01 mM, 0.02 mM, and 0.04 mM, to determine its effect on lymphocyte immunophenotype and function. Cells were also cultured in the presence of 0.01-0.04 mM magnesium acetate, Mg(CH3COO)(2) while still other cell samples were subjected to a mixture of Mg(CH3COO)(2) plus either Ni3S2 or NiSO4 at equimolar concentration. Following the culture, the immunophenotype of the cells was determined by indirect immunofluorescence, using monoclonal antibodies to major differentiation antigens of peripheral blood mononuclear cells, and their natural killer activity toward K562 target cells was measured. Both nickel salts were found to exert distinct effects on lymphocyte phenotype. Exposure of cells to Ni3S2 resulted in the decline of CD4 and natural killer cell populations. NiSO4 diminished the abundance of natural killer cells and, to a limited extent, also of CD4 cells. The nickel salts tested suppressed natural cytotoxicity of peripheral blood mononuclear cells, with Ni3S2 acting more strongly than NiSO4. The addition of Mg(CH3COO)(2) to a nickel salt during in vitro culture abolished the above inhibitory-effects. Nickel and magnesium salts did not affect CD3, CD8, CD20, and CD11a cell populations. The results indicate that nickel salts have deleterious effects on human peripheral blood mononuclear cells in short-term in vitro culture, but the magnitude of these effects varies, depending on the cell subsets. C1 NCI,FREDERICK CANC RES & DEV CTR,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21702. POZNAN UNIV,SCH MED,DEPT IMMUNOPATHOL,PL-60355 POZNAN,POLAND. NR 26 TC 13 Z9 14 U1 0 U2 1 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PD MAR 31 PY 1995 VL 97 IS 1-3 BP 39 EP 48 PG 10 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA QT012 UT WOS:A1995QT01200006 PM 7716791 ER PT J AU CLEAVELAND, ES MONKS, A VAIGROWOLFF, A ZAHAREVITZ, DW PAULL, K ARDALAN, K COONEY, DA FORD, H AF CLEAVELAND, ES MONKS, A VAIGROWOLFF, A ZAHAREVITZ, DW PAULL, K ARDALAN, K COONEY, DA FORD, H TI SITE OF ACTION OF 2 NOVEL PYRIMIDINE BIOSYNTHESIS INHIBITORS ACCURATELY PREDICTED BY THE COMPARE PROGRAM SO BIOCHEMICAL PHARMACOLOGY LA English DT Article DE BREQUINAR; BNID; DCL; CYTIDINE; URIDINE; MOLT-4 LYMPHOBLASTS; CHEMOTHERAPY; ANTIMETABOLITES ID TUMOR-CELL-LINES; DIHYDROOROTATE DEHYDROGENASE; BREQUINAR SODIUM; NUCLEOTIDE BIOSYNTHESIS; DENOVO; MECHANISM; CANCER AB The computer algorithm COMPARE provides information regarding the biological mechanism of action of a compound. In this study, excellent correlations were obtained for 2,2'-[3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)diimino]bis-benzoic acid (redoxal) and 1-(p-bromophenyl)-2-methyl-1H-naphth[2,3-d]imidazole-4,9-dione (BNID) and two well-studied dihydroorotate dehydrogenase (DHOD) inhibitors, dichloroallyl lawsone and brequinar, in terms of antiproliferative activity against tumor cell lines in vitro. When redoxal and BNID were incubated with MOLT-4 cells for 72 hr, 50% growth inhibition was achieved at 0.7 and 3.5 mu M, respectively. After 24 hr of incubation, pyrimidine triphosphate pools were shown to be decreased by 50% by redoxal (1 mu M) and BNID (0.25 mu M) Addition of either uridine (50 mu M) or cytidine (100 mu M) antagonized the cellular cytotoxicity caused by either drug; uridine corrected the UTP and CTP deficit, whereas cytidine corrected only the CTP deficit. Exposure of MOLT-4 cells to a 1 mu M concentration of either drug for 18 hr followed by a 1-hr exposure to [C-14]bicarbonate showed a 97% decrease of incorporation of [C-14] into pyrimidine triphosphates accompanied by a 91- and 82-fold increase in radioactive incorporation into L-dihydroorotate and N-carbamyl-L-aspartate, respectively. By direct exposure of DHOD prepared from MOLT-4 cell mitochondria to a range of concentrations of the two drugs, apparent K-i values of 0.33 mu M (redoxal) and 0.53 mu M (BNID) were determined. These data provide direct evidence for inhibition of DHOD by redoxal and BNID in MOLT-4 lymphoblasts. C1 NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,INFORMAT TECHNOL BRANCH,BETHESDA,MD 20892. NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,FREDERICK,MD 21702. RP CLEAVELAND, ES (reprint author), NCI,MED CHEM LAB,BLDG 37,ROOM 5B22,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 14 TC 34 Z9 34 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD MAR 30 PY 1995 VL 49 IS 7 BP 947 EP 954 DI 10.1016/0006-2952(95)00009-O PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QR216 UT WOS:A1995QR21600010 PM 7741767 ER PT J AU ADAMS, JD YAGI, H LEVIN, W JERINA, DM AF ADAMS, JD YAGI, H LEVIN, W JERINA, DM TI STEREOSELECTIVITY AND REGIOSELECTIVITY IN THE METABOLISM OF 7,8-DIHYDROBENZO[A]PYRENE BY CYTOCHROME-P450, EPOXIDE HYDROLASE AND HEPATIC MICROSOMES FROM 3-METHYLCHOLANTHRENE-TREATED RATS SO CHEMICO-BIOLOGICAL INTERACTIONS LA English DT Article DE 7,8-DIHYDROBENZO[A]PYRENE; BAY-REGION EPOXIDES; CYTOCHROME P450 1A1; EPOXIDE HYDROLASE ID DIOL-EPOXIDES; STEREOSELECTIVE METABOLISM; EXCEPTIONAL ACTIVITY; LIVER ENZYMES; MUTAGENICITY; BENZOPYRENE; BENZANTHRACENE; 7,8-DIHYDRODIOL; TUMORIGENICITY; CHROMATOGRAPHY AB The active site of cytochrome P450 1A1 has been probed with the substrate, 7,8-dihydrobenzo[a]pyrene using a purified, reconstituted system composed of cytochrome P450 1A1, NADPH-cytochrome c reductase and lipid in the presence or absence of epoxide hydrolase. The turnover of the substrate was found to be 38 nmol/nmol of cytochrome P450/min. The metabolic products that were identified are: a phenolic 7,8-dihydrobenzo[a]pyrene (20-29%); 9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (17-28%); benzo[a]pyrene (12-19%); 7-hydroxy-7,8-dihydrobenzo[a]pyrene (13-16%); 8-hydroxy-7,8-dihydrobenzo[a]pyrene (7-15%); 3-hydroxybenzo[a]pyrene (7-15%); 4,5-epoxy-4,5,7,8-tetrahydrobenzo[a]pyrene (0-4%); and a triol of 7,8,9,10-tetrahydrobenzo[a]pyrene (0-4%). 9,10-Epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene undergoes rapid hydrolysis to cis- and trans-9,10-dihydroxy-7,8,9,1O-tetrahydrobenzo[a]pyrene (2:1) by benzylic attack of water at C-10. Approximately 71% of the trans diols are derived from (+)-(9S,10R)-9,10-epoxy-7,8,9,1O-tetrahydrobenzo[a]pyrene, indicating that cytochrome P450 1A1 has more than a 2:1 preference for selective epoxidation of an enantiotopic face of 7,8-dihydrobenzo[a]pyrene. This stereo-selectivity agrees with the postulated stereo-selectivity predicted by a previously described active site model for cytochrome P450 1A1. Epoxide hydrolase in pure form or in hepatic microsomes catalyzes the hydrolysis of 9,10-epoxy-7,8,9, 10-tetrahydrobenzo[a]pyrene, which is inhibited by 1,1,1-trichloropropane 2,3-oxide. The (+)-(9S,10R)-isomer of the epoxide is slightly preferred as a substrate over its enantiomer and is cleaved by benzylic and nonbenzylic attack. Only benzylic attack was found with (-)-(9R,10S)-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene. C1 NIDDKD,BIOORGAN CHEM LAB,BETHESDA,MD 20892. HOFFMANN LA ROCHE INC,DEPT INFLAMMAT AUTO IMMUNE DIS,NUTLEY,NJ 07110. RP ADAMS, JD (reprint author), UNIV SO CALIF,SCH PHARM,1985 ZONAL AVE,PSC 508,LOS ANGELES,CA 90033, USA. NR 41 TC 12 Z9 13 U1 0 U2 0 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0009-2797 J9 CHEM-BIOL INTERACT JI Chem.-Biol. Interact. PD MAR 30 PY 1995 VL 95 IS 1-2 BP 57 EP 77 DI 10.1016/0009-2797(94)03354-4 PG 21 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology GA QL916 UT WOS:A1995QL91600005 PM 7697754 ER PT J AU MTIMAVALYE, L BIGGAR, RJ TAHA, TE CHIPHANGWI, J AF MTIMAVALYE, L BIGGAR, RJ TAHA, TE CHIPHANGWI, J TI MATERNAL-INFANT TRANSMISSION OF HIV-1 SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 NCI,BETHESDA,MD 20852. JOHNS HOPKINS UNIV,MINIST HLTH RES PROJECT,BALTIMORE,MD 21218. UNIV MALAWI,SCH MED,BLANTYRE,MALAWI. RP MTIMAVALYE, L (reprint author), QUEEN ELIZABETH CENT HOSP,BLANTYRE,MALAWI. NR 4 TC 6 Z9 6 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 30 PY 1995 VL 332 IS 13 BP 890 EP 891 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA QP228 UT WOS:A1995QP22800019 PM 7726969 ER PT J AU CONNOR, E SPERLING, R GELBER, RD BALSLEY, J AF CONNOR, E SPERLING, R GELBER, RD BALSLEY, J TI MATERNAL-INFANT TRANSMISSION OF HIV-1 - REPLY SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 CUNY MT SINAI SCH MED,NEW YORK,NY 10029. HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. NIAID,BETHESDA,MD 20892. RP CONNOR, E (reprint author), MEDIMMUNE INC,GAITHERSBURG,MD 20878, USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 30 PY 1995 VL 332 IS 13 BP 891 EP 891 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA QP228 UT WOS:A1995QP22800020 ER PT J AU STRICKLER, HD RATTRAY, C ESCOFFERY, C MANNS, A SCHIFFMAN, MH BROWN, C CRANSTON, B HANCHARD, B PALEFSKY, JM BLATTNER, WA AF STRICKLER, HD RATTRAY, C ESCOFFERY, C MANNS, A SCHIFFMAN, MH BROWN, C CRANSTON, B HANCHARD, B PALEFSKY, JM BLATTNER, WA TI HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-I AND SEVERE NEOPLASIA OF THE CERVIX IN JAMAICA SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID HUMAN PAPILLOMAVIRUS INFECTION; CANCER AB Human T-cell lymphotropic virus type I (HTLV-I) was associated with carcinoma of the cervix in Japan in a recent study that compared hospital cases with healthy population-based controls. To test this relationship in women more alike for cervical neoplasia risk factors (including sexual behavior and human papilloma virus; HPV), we enrolled consecutive patients from a colposcopy clinic in Kingston, Jamaica (an HTLV-I endemic area). Patients underwent Pap smear, colposcopy, biopsy and cervical swab for detection of HPV by polymerase chain reaction. Cases were defined as women with CIN-3 or invasive cancer (CIN-3/CA). Controls included all patients with either CIN-I or koilocytotic atypia, atypical squamous cells of undetermined significance or benign cervical pathology (all but one had at least inflammatory changes). Patients with CIN-2 were excluded to minimize risk of case-control misclassification. Cases were much more likely to be HTLV-I seropositive than controls. Although mean age differed significantly between cases (mean age 39 years) and controls (mean age = 33 years), control for age did not explain the relation of CIN-3/CA with HTLV-I. Among HPV DNA positive subjects the age-adjusted association was not diminished but lost statistical significance. HTLV-I seroprevalence may be independently associated with progression to severe neoplasia of the cervix. (C) 1995 Wiley-Liss, Inc* C1 NATL CANC INST,ENVIRONM EPIDEMIOL BRANCH,ROCKVILLE,MD 20852. UNIV W INDIES,DEPT PATHOL,KINGSTON 7,JAMAICA. UNIV W INDIES,DEPT GYNECOL,KINGSTON 7,JAMAICA. UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143. RP STRICKLER, HD (reprint author), NATL CANC INST,VIRAL BRANCH,6130 EXECUT BLVD,EPN 434,ROCKVILLE,MD 20852, USA. NR 22 TC 21 Z9 21 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD MAR 29 PY 1995 VL 61 IS 1 BP 23 EP 26 DI 10.1002/ijc.2910610105 PG 4 WC Oncology SC Oncology GA QQ317 UT WOS:A1995QQ31700004 PM 7705929 ER PT J AU CARDINALI, M PIETRASZKIEWICZ, H ENSLEY, JF ROBBINS, KC AF CARDINALI, M PIETRASZKIEWICZ, H ENSLEY, JF ROBBINS, KC TI TYROSINE PHOSPHORYLATION AS A MARKER FOR ABERRANTLY REGULATED GROWTH-PROMOTING PATHWAYS IN CELL-LINES DERIVED FROM HEAD AND NECK MALIGNANCIES SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID FACTOR RECEPTOR GENE; FACTOR-ALPHA; AMPLIFICATION; EXPRESSION; KINASES; TRANSFORMATION; CARCINOMAS; ONCOGENE; CANCER AB We have utilized a broad approach to address whether tyrosine kinases and the growth pathways they regulate might be functionally aberrant in squamous cell carcinomas (SCC) of the upper aerodigestive tract. This strategy involved assaying for evidence of tyrosine kinase action in lysates of cell lines representing SCC. Our findings revealed a spectrum of elevated tyrosine phosphorylation in SCC lines ranging from less than 2-fold to more than 10-fold above that of control human epidermal keratinocytes. Thus the ability to regulate growth and other pathways controlled by tyrosine phosphorylation was impaired in all the 19 lines examined. Assessment of the receptor for epidermal growth factor (EGF) revealed that its activity was elevated above normal in 14 of the 19 cell lines examined, suggesting that at least a portion of the increased tyrosine phosphorylation observed could be attributed to excessive EGF receptor activity. Our findings provide functional evidence that growth pathways are aberrantly regulated in cell lines representing SCC of the upper aerodigestive tract. (C) 1995 Wiley-Liss, Inc.* C1 NIDR,CELLULAR DEV & ONCOL LAB,BETHESDA,MD 20892. WAYNE STATE UNIV,HARPER HOSP,DEPT INTERNAL MED,DIV HEMATOL & ONCOL,DETROIT,MI 48201. NR 25 TC 86 Z9 86 U1 0 U2 3 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD MAR 29 PY 1995 VL 61 IS 1 BP 98 EP 103 DI 10.1002/ijc.2910610117 PG 6 WC Oncology SC Oncology GA QQ317 UT WOS:A1995QQ31700016 PM 7705939 ER PT J AU BUBB, MR KORN, ED AF BUBB, MR KORN, ED TI KINETIC-MODEL FOR THE INHIBITION OF ACTIN POLYMERIZATION BY ACTOBINDIN SO BIOCHEMISTRY LA English DT Article ID ACANTHAMOEBA ACTOBINDIN; F-ACTIN; BINDING AB Although Acanthamoeba actobindin binds actin monomers, its inhibition of actin polymerization differs from that of a simple monomer-sequestering protein in that actobindin inhibits nucleation very much more than elongation [Lambooy, P. K., and Korn, E. D. (1988) J. Biol, Chem. 263, 12836-12843] and can induce the accumulation of actin dimers in stoichiometric excess of the actobindin concentration [Bubb, M. R., Knutson, J. R., Porter, D. M,, and Kern, E. D. (1994) J. Biol. Chem, 269, 25592-25597]. We now describe a ''catalytic'' model for the interaction of actobindin with actin monomer that quantitatively accounts for the effects of actobindin on the kinetics of actin polymerization de novo and the elongation of actin filaments. We propose that, in a polymerizing buffer, actobindin binds to two actin subunits forming an heterotrimeric complex that is incompetent for nucleation, self-association, and elongation. Actobindin can, however, dissociate from this complex, leaving a novel actin dimer that can participate in elongation but remains incompetent for nucleation and self-association. Under appropriate conditions, the concentration of this novel actin dimer can exceed the actobindin concentration; thus, the model is catalytic rather than stoichiometric. The experimentally observed time course of actin polymerization de novo, the rate of elongation of filaments, and the amount of actin dimer formed as a function of actobindin concentration are all consistent with the catalytic model and inconsistent with the stoichiometric model. The rate of actobindin-induced actin dimer formation is consistent with the hypothesis that the rate-limiting step is this pathway is the formation of a precursor heterotrimeric complex. C1 NHLBI, CELL BIOL LAB, BETHESDA, MD 20892 USA. RI Korn, Edward/F-9929-2012 NR 14 TC 12 Z9 12 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 28 PY 1995 VL 34 IS 12 BP 3921 EP 3926 DI 10.1021/bi00012a008 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QP971 UT WOS:A1995QP97100008 PM 7696256 ER PT J AU GROVER, S RIMOLDI, JM MOLINERO, AA CHAUDHARY, AG KINGSTON, DGI HAMEL, E AF GROVER, S RIMOLDI, JM MOLINERO, AA CHAUDHARY, AG KINGSTON, DGI HAMEL, E TI DIFFERENTIAL-EFFECTS OF PACLITAXEL (TAXOL) ANALOGS MODIFIED AT POSITIONS C-2, C-7, AND C-3' ON TUBULIN POLYMERIZATION AND POLYMER STABILIZATION - IDENTIFICATION OF A HYPERACTIVE PACLITAXEL DERIVATIVE SO BIOCHEMISTRY LA English DT Article ID MICROTUBULE-ASSOCIATED PROTEINS; BIOLOGICAL EVALUATION; ASSEMBLY INVITRO; TAXOTERE(R); PRECURSOR; TAXANES; BINDS; NMR AB Our finding that an analog of paclitaxel (Taxol) modified at position C-2 (2-debenzoyl-2-(m-azidobenzoyl)paclitaxel) was substantially more active than paclitaxel in promoting tubulin assembly [Chaudhary et al. (1994) J. Am. Chem. Sec. 116, 4097-4098] led us to perform an analysis of the modulating effects of microtubule-associated proteins, GTP, and temperature on assembly and polymer stability. The analog always showed superior activity to paclitaxel in inducing polymerization where it fails to occur without drug: probably indicating a greater ability than paclitaxel to ''hypernucleate'' assembly. In contrast, much smaller differences in effects on polymer stability were observed. The analysis was extended to a large series of derivatives modified at positions C-2, C-7, C-10, and C-3', including docetaxel, a clinically important analog of paclitaxel. While analog stabilization of polymer was frequently observed, neither qualitative nor quantitative analysis of this property reliably predicted whether a compound would have enhanced hypernucleation activity relative to that of paclitaxel. Stabilization was often observed at substoichiometric analog concentrations, while even superstoichiometric concentrations of most compounds failed to induce extensive tubulin polymerization at low temperatures or in the absence of microtubule-associated proteins or GTP. Docetaxel was intermediate in activity between paclitaxel and 2-debenzoyl-2-(m-azidobenzoyl)paclitaxel in promoting assembly reactions, We conclude that the hypernucleation of tubulin assembly and polymer stabilization observed with paclitaxel represent two distinct properties of the drug. Our findings suggest that paclitaxel, docetaxel, and 2-debenzoyl-2-(m-azidobenzoyl)paclitaxel are able to interact with progressively smaller assemblages of tubulin at low temperatures or in the absence of microtubule-associated proteins or GTP. C1 NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. VIRGINIA POLYTECH INST & STATE UNIV,DEPT CHEM,BLACKSBURG,VA 24061. OI Kingston, David/0000-0001-8944-246X FU NCI NIH HHS [CA-48974, CA-55131] NR 40 TC 37 Z9 37 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA PO BOX 57136, WASHINGTON, DC 20037-0136 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 28 PY 1995 VL 34 IS 12 BP 3927 EP 3934 DI 10.1021/bi00012a009 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QP971 UT WOS:A1995QP97100009 PM 7696257 ER PT J AU AOKI, I KINZER, C SHIRAI, A PAUL, WE KLINMAN, DM AF AOKI, I KINZER, C SHIRAI, A PAUL, WE KLINMAN, DM TI IGE - RECEPTOR-POSITIVE NON-B/NON-T CELLS DOMINATE THE PRODUCTION OF INTERLEUKIN-4 AND INTERLEUKIN-6 IN IMMUNIZED MICE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID SPLENIC NON-B; INTERFERON-GAMMA; FC-EPSILON; MURINE LEISHMANIASIS; STIMULATING FACTOR; CROSS-LINKAGE; IL-4; LYMPHOKINES; EXPRESSION; BASOPHILS AB The phenotype and antigenic specificity of cells secreting interleukin (IL) 4, IL-6, and interferon gamma was studied in mice during primary and secondary immune responses. T lymphocytes were the major source of interferon gamma, whereas non-B/non-T cells were the dominant source of IL-4 and IL-6 in the spleens of immunized animals. Cytokine-secreting non-B/non-T cells expressed surface receptors for IgE and/or IgG types II/III. Exposing these cells to antigen-specific IgE or IgG in vivo (or in vitro) ''armed'' them to release IL-4 and IL-6 upon subsequent antigenic challenge. These findings suggest that non-B/non-T cells may represent the ''natural immunity'' analogue of CD4(+) T helper type 2 cells and participate in a positive feedback loop involved in the perpetuation of T helper type 2 cell responses. C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. RP AOKI, I (reprint author), US FDA,CTR BIOL EVALUAT & RES,DIV VIRAL PROD,RETROVIRAL IMMUNOL SECT,BETHESDA,MD 20892, USA. NR 39 TC 37 Z9 37 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 2534 EP 2538 DI 10.1073/pnas.92.7.2534 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900026 PM 7708680 ER PT J AU SANDERSON, N FACTOR, V NAGY, P KOPP, J KONDAIAH, P WAKEFIELD, L ROBERTS, AB SPORN, MB THORGEIRSSON, SS AF SANDERSON, N FACTOR, V NAGY, P KOPP, J KONDAIAH, P WAKEFIELD, L ROBERTS, AB SPORN, MB THORGEIRSSON, SS TI HEPATIC EXPRESSION OF MATURE TRANSFORMING GROWTH-FACTOR-BETA-1 IN TRANSGENIC MICE RESULTS IN MULTIPLE TISSUE LESIONS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID FACTOR-ALPHA; TGF-BETA; FACTOR-BETA-1; LIVER; PURIFICATION; INDUCTION; FIBROSIS; SITE AB Aberrant expression of transforming growth factor beta 1 (TGF-beta 1) has been implicated in a number of disease processes, particularly those involving fibrotic and inflammatory lesions. To determine the in vivo effects of overexpression of TGF-beta 1 on the function and structure of hepatic as well as extrahepatic tissues, transgenic mice were generated containing a fusion gene (Alb/TGF-beta 1) consisting of modified porcine TGF-beta 1 cDNA under the control of the regulatory elements of the mouse albumin gene. Five transgenic lines were developed, all of which expressed the Alb/TGF-beta 1 transgene selectively in hepatocytes. The transgenic line 25 expressing the highest level of the transgene in the liver also had high (>10-fold over control) plasma levels of TGF-beta 1. Hepatic fibrosis and apoptotic death of hepatocytes developed in all the transgenic lines but was more pronounced in line 25. The fibrotic process was characterized by deposition of collagen around individual hepatocytes and within the space of Disse in a radiating linear pattern. Several extrahepatic lesions developed in line 25, including glomerulonephritis and renal failure, arteritis and myocarditis, as well as atrophic changes in pancreas and testis. The results from this transgenic model strongly support the proposed etiological role for TGF-beta 1 in a variety of fibrotic and inflammatory disorders, The transgenic model may also provide an appropriate paradigm for testing therapeutic interventions aimed at neutralizing the detrimental effects of this important cytokine. C1 NCI, EXPTL CARCINOGENESIS LAB, BETHESDA, MD 20892 USA. NCI, DIV CANC ETIOL, CHEMOPREVENT LAB, BETHESDA, MD 20892 USA. NIDR, ORAL MED LAB, BETHESDA, MD 20892 USA. NR 27 TC 515 Z9 531 U1 1 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 2572 EP 2576 DI 10.1073/pnas.92.7.2572 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900034 PM 7708687 ER PT J AU YUAN, DS STEARMAN, R DANCIS, A DUNN, T BEELER, T KLAUSNER, RD AF YUAN, DS STEARMAN, R DANCIS, A DUNN, T BEELER, T KLAUSNER, RD TI THE MENKES-WILSON-DISEASE GENE HOMOLOG IN YEAST PROVIDES COPPER TO A CERULOPLASMIN-LIKE OXIDASE REQUIRED FOR IRON UPTAKE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID SACCHAROMYCES-CEREVISIAE; CANDIDATE GENE; TRANSPORTING ATPASE; FERRIC REDUCTASE; PROTEIN; METABOLISM; ENCODES; CLONING AB The CCC2 gene of the yeast Saccharomyces cerevisiae is homologous to the human genes defective in Wilson disease and Menkes disease. A biochemical hallmark of these diseases is a deficiency of copper in ceruloplasmin and other copper proteins found in extracytosolic compartments. Here we demonstrate that disruption of the yeast CCC2 gene results in defects in respiration and iron uptake. These defects could be reversed by supplementing cells with copper. suggesting that CCC2 mutant cells were copper deficient, However, cytosolic copper levels and copper uptake were normal. Instead, CCC2 mutant cells lacked a copper-dependent oxidase activity associated with the estracytosolic domain of the FET3-encoded protein, a ceruloplasmin homologue previously shown to be necessary for high-affinity iron uptake in yeast. Copper restored oxidase activity both in vitro and in vivo, paralleling the ability of copper to restore respiration and iron uptake. These results suggest that the CCC2-encoded protein is required for the export of copper from the cytosol into an estracytosolic compartment, supporting the proposal that intracellular copper transport is impaired in Wilson disease and Menkes disease. C1 NICHHD, CELL BIOL & METAB BRANCH, BETHESDA, MD 20892 USA. UNIFORMED SERV UNIV HLTH SCI, DEPT BIOCHEM, BETHESDA, MD 20814 USA. NR 40 TC 358 Z9 363 U1 2 U2 8 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 2632 EP 2636 DI 10.1073/pnas.92.7.2632 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900046 PM 7708696 ER PT J AU PASTAN, IH ARCHER, GE MCLENDON, RE FRIEDMAN, HS FUCHS, HE WANG, QC PAI, LH HERNDON, J BIGNER, DD AF PASTAN, IH ARCHER, GE MCLENDON, RE FRIEDMAN, HS FUCHS, HE WANG, QC PAI, LH HERNDON, J BIGNER, DD TI INTRATHECAL ADMINISTRATION OF SINGLE-CHAIN IMMUNOTOXIN, LMB-7 [B3(FV)-PE38], PRODUCES CURES OF CARCINOMATOUS MENINGITIS IN A RAT MODEL SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID MONOCLONAL-ANTIBODIES; NEOPLASTIC MENINGITIS; TUMOR XENOGRAFTS; FV; BINDING; MACROMOLECULES; PENETRATION; FRAGMENT; THERAPY; MICE AB LMB-7 [B3(Fv)-PE38] is a single-chain immunotoxin constructed from the murine monoclonal antibody B3 and a truncated form of Pseudomonas exotoxin PE38. Antibody B3 recognizes a carbohydrate epitope found on solid tumors that frequently invade the intrathecal space and cause neoplastic meningitis. We tested the therapeutic value of intrathecally administered LMB-7 by using a model of human neoplastic meningitis in athymic rats. This model is representative of a clinical situation in that antibody B3 cross-reacts with a number of normal tissues that can be used to monitor potential systemic toxicity. Treatment was begun 3 days after A431 tumor implantation. Without treatment, the animals median survival was 10 days. Intrathecal administration of 10 mu g of LMB-7 in 40 mu l on days 3, 5, and 7 produced 4 of 10 and 8 of 10 long-term survivors (> 170 days) in two experiments. Of the long-term survivors, 2 of 4 and 7 of 8 survivors had no microscopic evidence of tumor and were considered histologic cures. Lack of significant toxicity in the effective dose range and specificity make LMB-7 an excellent candidate for intrathecal treatment of neoplastic meningitis in humans. C1 DUKE UNIV,MED CTR,DEPT PATHOL,DURHAM,NC 27710. DUKE UNIV,MED CTR,PREUSS LAB BRAIN TUMOR RES,DURHAM,NC 27710. DUKE UNIV,MED CTR,DIV BIOMETRY,DURHAM,NC 27710. RP PASTAN, IH (reprint author), NCI,DIV CANC BIOL DIAG & CTR,MOLEC BIOL LAB,37 CONVENT DR,MSC 4255,BETHESDA,MD 20892, USA. FU NCI NIH HHS [CA 56115, CA 11898]; NINDS NIH HHS [NS 20023] NR 31 TC 33 Z9 35 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 2765 EP 2769 DI 10.1073/pnas.92.7.2765 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900073 PM 7708720 ER PT J AU MATTHEW, E ANDREASON, P PETTIGREW, K CARSON, RE HERSCOVITCH, P COHEN, R KING, C JOHANSON, CE GREENBLATT, DJ PAUL, SM AF MATTHEW, E ANDREASON, P PETTIGREW, K CARSON, RE HERSCOVITCH, P COHEN, R KING, C JOHANSON, CE GREENBLATT, DJ PAUL, SM TI BENZODIAZEPINE RECEPTORS MEDIATE REGIONAL BLOOD-FLOW CHANGES IN THE LIVING HUMAN BRAIN SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE (H2O)-O-15 POSITRON-EMISSION TOMOGRAPHY ID POSITRON EMISSION TOMOGRAPHY; CEREBRAL GLUCOSE-UTILIZATION; CENTRAL NERVOUS-SYSTEM; ANTAGONIST RO 15-1788; DIAZEPAM; METABOLISM; BINDING; LORAZEPAM; AGONISTS; DISORDER AB We studied the effects of a high-affinity gamma-aminobutyric acid (GABA) -benzodiazepine-receptor agonist (lorazepam) and an antagonist (flumazenil) in humans, using (H2O)-O-15 positron-emission tomography. Administration of lorazepam to healthy volunteers caused time- and dose-dependent reductions in regional cerebral blood flow and self-reported alterations in behavioral/mood parameters. Flumazenil administration reversed these changes. These observations indicated that benzodiazepine-induced effects on regional cerebral blood flow and mood/behavior are mediated at some level through GABA-benzodiazepine receptors, although the specific mechanism remains unclear. The approach described here provides a method for quantifying GABA-benzodiazepine-receptor-mediated neurotransmission in the living human brain and mag be useful for studying the role of these receptors in a variety of neuropsychiatric disorders. C1 NIMH,CLIN NEUROSCI BRANCH,BETHESDA,MD 20892. NIMH,CLIN BRAIN IMAGING SECT,CEREBRAL BLOOD FLOW & METAB LAB,BETHESDA,MD 20892. NIMH,DIV EPIDEMIOL & SERV RES,BETHESDA,MD 20892. NIH,DEPT NUCL MED,PET SECT,BETHESDA,MD 20892. NIH,DEPT NUCL MED,PET SECT,BETHESDA,MD 20892. UNIFORMED SERV UNIV HLTH SCI,DEPT PSYCHIAT,BETHESDA,MD 20215. TUFTS UNIV,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,BOSTON,MA 02111. RI Carson, Richard/H-3250-2011 OI Carson, Richard/0000-0002-9338-7966 NR 32 TC 35 Z9 36 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 2775 EP 2779 DI 10.1073/pnas.92.7.2775 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900075 PM 7708722 ER PT J AU PEOPLES, RW WEIGHT, FF AF PEOPLES, RW WEIGHT, FF TI CUTOFF IN POTENCY IMPLICATES ALCOHOL INHIBITION OF N-METHYL-D-ASPARTATE RECEPTORS IN ALCOHOL-INTOXICATION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE GLUTAMATE RECEPTOR; ION CHANNEL; NEUROTRANSMITTER ID LONG-TERM POTENTIATION; CHANNEL COMPLEX; CHAIN ALCOHOLS; MEMBRANES; DRUGS AB As the number of carbon atoms in an aliphatic n-alcohol is increased from one to five, intoxicating potency, lipid solubility, and membrane lipid disordering potency all increase in a similar exponential manner. However, the potency of aliphatic n-alcohols for producing intoxication reaches a maximum at six to eight carbon atoms and then decreases. The molecular basis of this ''cutoff'' effect is not understood, as it is not correlated with either the lipid solubility or the membrane disordering potency of the alcohols, which continue to increase exponentially. Since it has been suggested that inhibition of N-methyt-D-aspartate (NR-IDA) receptors by alcohols may play a role in alcohol intoxication, we investigated whether a series of aliphatic n-alcohols would exhibit a cutoff in potency for inhibition of NMDA receptors. We found that although potency for inhibition of NMDA receptors increased exponentially for alcohols with one to five carbon atoms, potency for inhibition of NMDA receptors reached a maximum at six to eight carbon atoms and then abruptly disappeared. This cutoff for alcohol inhibition of NMDA receptors is consistent with an interaction of the alcohols with a hydrophobic pocket on the receptor protein. In addition, the similarity of the cutoffs for alcohol inhibition of NMDA receptors and alcohol intoxication suggests that the cutoff for NMDA receptor inhibition may contribute to the cutoff for alcohol intoxication, which is consistent with an important role of NMDA receptors in alcohol intoxication. RP PEOPLES, RW (reprint author), NIAAA,MOLEC & CELLULAR NEUROBIOL LAB,12501 WASHINTON AVE,ROCKVILLE,MD 20852, USA. NR 30 TC 97 Z9 98 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 2825 EP 2829 DI 10.1073/pnas.92.7.2825 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900085 PM 7708732 ER PT J AU WOODWORTH, CD MCMULLIN, E IGLESIAS, M PLOWMAN, GD AF WOODWORTH, CD MCMULLIN, E IGLESIAS, M PLOWMAN, GD TI INTERLEUKIN-1-ALPHA AND TUMOR-NECROSIS-FACTOR-ALPHA STIMULATE AUTOCRINE AMPHIREGULIN EXPRESSION AND PROLIFERATION OF HUMAN PAPILLOMAVIRUS-IMMORTALIZED AND CARCINOMA-DERIVED CERVICAL EPITHELIAL-CELLS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE KERATINOCYTE; EPIDERMAL GROWTH FACTOR; PROINFLAMMATORY CYTOKINES; CERVICAL CANCER ID GROWTH FACTOR-ALPHA; HUMAN KERATINOCYTES; TRANSFORMED-CELLS; HUMAN-FIBROBLASTS; FACTOR RECEPTOR; CANCER; TYPE-16; LINES; INFLAMMATION; CYTOKINES AB Infection with multiple sexually transmitted agents has been associated with inflammation of the cervix and an increased risk of cervical cancer in women infected with human papillomaviruses (HPVs). Two proinflammatory cytokines, interleukin 1 alpha (IL-1 alpha) and tumor necrosis factor alpha (TNF-alpha), inhibited proliferation of normal epithelial cells cultured from human cervix. In contrast, both cytokines significantly stimulated proliferation of cervical cell lines (5 of 7) immortalized by transfection with HPV-16 or -18 DNAs or lines derived from cervical carcinomas (7 of 11). Stimulation was dose dependent from 0.01 to 1.0 nM and was blocked by specific inhibitors, such as the IL-1 receptor antagonist or the TNF type 1 or 2 soluble receptors. Growth stimulation by IL-1 alpha or TNF-alpha was accompanied by a 6- to 10-fold increase in RNA encoding amphiregulin, an epidermal growth factor (EGF) receptor ligand. Recombinant human amphiregulin (0.1 nM) was as effective as IL-1 alpha or TNF-alpha in promoting proliferation. Monoclonal antibodies that blocked signal transduction by the EGF receptor or that neutralized amphiregulin activity prevented mitogenic stimulation by IL-1 alpha or TNF-alpha. These studies indicate that IL-1 alpha and TNF-alpha stimulate proliferation of immortal and malignant cervical epithelial cells by an EGF receptor-dependent pathway requiring autocrine stimulation by amphiregulin. Furthermore, they suggest that chronic inflammation and release of proinflammatory cytokines might provide a selective growth advantage for abnormal cervical cells in vivo. C1 SUGEN INC,REDWOOD CITY,CA 94063. RP WOODWORTH, CD (reprint author), NCI,BIOL LAB,BETHESDA,MD 20892, USA. RI PLOWMAN, Greg/E-2012-2011 NR 35 TC 110 Z9 113 U1 0 U2 6 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 2840 EP 2844 DI 10.1073/pnas.92.7.2840 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900088 PM 7708734 ER PT J AU SCHWAN, TG PIESMAN, J GOLDE, WT DOLAN, MC ROSA, PA AF SCHWAN, TG PIESMAN, J GOLDE, WT DOLAN, MC ROSA, PA TI INDUCTION OF AN OUTER SURFACE PROTEIN ON BORRELIA-BURGDORFERI DURING TICK FEEDING SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE IXODES SCAPULARIS; LYME DISEASE; BLOOD MEAL; OUTER SURFACE PROTEIN C; TEMPERATURE ID LYME-DISEASE; IXODES-DAMMINI; MONOCLONAL-ANTIBODY; OSPC GENE; TRANSMISSION; EXPRESSION; VIRULENCE; LIPOPROTEIN; ATTACHMENT; BACTERIA AB Lyme disease spirochetes, Borrelia burgdorferi sensu lato, are maintained in zoonotic cycles involving ticks and small mammals. In unfed ticks, the spirochetes produce one outer surface protein, OspA, but not OspC. During infection in mammals, immunological data suggest that the spirochetes have changed their surface, now expressing OspC but little or no OspA. We find by in vitro growth experiments that this change is regulated in part by temperature; OspC is produced by spirochetes at 32-37 degrees C but not at 24 degrees C. Furthermore, spirochetes in the midgut of ticks that have fully engorged on mice now have OspC on their surface. Thus two environmental cues, an increase in temperature and tick feeding, trigger a major alteration of the spirochetal outer membrane. This rapid synthesis of OspC by spirochetes during tick feeding may play an essential role in the capacity of these bacteria to successfully infect mammalian hosts, including humans, when transmitted by ticks. C1 CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV VECTOR BORNE INFECT DIS,FT COLLINS,CO 80522. RP SCHWAN, TG (reprint author), NIAID,ROCKY MT LABS,MICROBIAL STRUCT & FUNCT LAB,HAMILTON,MT 59840, USA. NR 46 TC 638 Z9 644 U1 0 U2 27 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 2909 EP 2913 DI 10.1073/pnas.92.7.2909 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900102 PM 7708747 ER PT J AU YANG, YL BAILEY, J VACCHIO, MS YARCHOAN, R ASHWELL, JD AF YANG, YL BAILEY, J VACCHIO, MS YARCHOAN, R ASHWELL, JD TI RETINOIC ACID INHIBITION OF EX-VIVO HUMAN IMMUNODEFICIENCY VIRUS-ASSOCIATED APOPTOSIS OF PERIPHERAL-BLOOD CELLS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID ACTIVATION-INDUCED APOPTOSIS; ACUTE PROMYELOCYTIC LEUKEMIA; MATURE T-CELLS; DEATH APOPTOSIS; HIV-INFECTION; EXPRESSION; AIDS; DYSFUNCTION; RECEPTOR; ANTIGEN AB T cells from human immunodeficiency virus (HIV)-infected individuals undergo spontaneous and activation-induced ex vivo apoptosis, Here we report that peripheral blood mononuclear cells (PBMCs) obtained from six HIV-infected individuals exhibited reduced ex vivo DNA fragmentation and cell death after ingestion of all-trans-retinoic acid (tRA). These effects were attenuated with continued daily RA administration, which correlated with a >5-fold decrease in serum peak RA concentrations, Incubation of PBMCs from HIV+ individuals with tRA in vitro resulted in decreased DNA fragmentation in a subset of patients, especially those having <500 CD4(+) T cells per mm(3). tRA also inhibited apoptosis of preactivated normal PBMCs induced to die by restimulation, which raises the possibility of a common mechanism between activation-induced apoptosis of activated normal PBMCs and apoptosis associated with HIV infection, Whether HIV-associated apoptosis of PBMCs, and its prevention by RA, has an impact on T-cell survival or the course of disease in patients infected with HIV will require further evaluation. C1 NCI,CLIN ONCOL PROGRAM,BETHESDA,MD 20892. RP YANG, YL (reprint author), NCI,BIOL RESPONSE MODIFIERS PROGRAM,IMMUNE CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 39 TC 44 Z9 46 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 3051 EP 3055 DI 10.1073/pnas.92.7.3051 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900131 PM 7708773 ER PT J AU HONG, JX ZHANG, XK MOSS, J VAUGHAN, M AF HONG, JX ZHANG, XK MOSS, J VAUGHAN, M TI ISOLATION OF AN AMINO-TERMINAL DELETED RECOMBINANT ADP-RIBOSYLATION FACTOR-1 IN AN ACTIVATED NUCLEOTIDE-FREE STATE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID GUANINE-NUCLEOTIDE; CHOLERA-TOXIN; BINDING PROTEINS; BREFELDIN-A; GOLGI MEMBRANES; BOVINE BRAIN; HUMAN GENE; GTP; ARF; RIBOSYLTRANSFERASE AB ADP-ribosylation factors (ARFs) are approximate to 20-kDa guanine nucleotide-binding proteins that activate cholera toxin ADP-ribosyltransferase in vitro and participate in intracellular vesicular membrane trafficking. ARFs are activated when bound GDP is replaced by GTP and inactivated by hydrolysis of bound GTP to yield ARF-GDP. Usually, ARFs are isolated in an inactive GDP-bound state and require addition of GTP along with detergent or phospholipid for activity, Purified mutant recombinant ARF1 lacking the first 13 amino acids (r Delta 13ARF1-P) stimulated cholera toxin activity essentially equally with or without added GTP (and phospholipid or detergent), at least in part due to the presence of bound nucleotides, which later were identified as GTP and GDP. Nucleotide-free r Delta 13ARF1 (r Delta 13ARF1-F), prepared by dialysis against 7 M urea, was active without added GTP in the absence of SDS but inactive without added GTP in its presence. Renaturation of r Delta 13ARF1-F in the presence of GTP, ITP, or GDP yielded, respectively, r Delta 13ARF1-GTP and r Delta 13ARF1-ITP, which were active, and r Delta 13ARF1-GDP, which was inactive, Effects of phospholipids and detergents on nucleotide exchangeability evaluated as effects on activity of rARF1 and r Delta 13ARF1-F differed. With r Delta 13ARF1-F, 100 mu M ITP and 100 mu M GTP were essentially equally effective in the presence of cardiolipin or SDS. The finding that r Delta 13ARF1 differs from rARF1 in the effects of phospholipids and detergents on nucleotide binding is consistent with the conclusion that the ARF amino terminus plays an important role in nucleotide binding and its specificity as well as the molecular conformation and associated activity, C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP HONG, JX (reprint author), NHLBI,PULM CRIT CARE MED BRANCH,BLDG 10,BETHESDA,MD 20892, USA. NR 28 TC 12 Z9 15 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 28 PY 1995 VL 92 IS 7 BP 3056 EP 3059 DI 10.1073/pnas.92.7.3056 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QP889 UT WOS:A1995QP88900132 PM 7708774 ER PT J AU SEPEHRNIA, B PREZANT, TR ROTTER, JI PETTITT, DJ KNOWLER, WC FISCHELGHODSIAN, N AF SEPEHRNIA, B PREZANT, TR ROTTER, JI PETTITT, DJ KNOWLER, WC FISCHELGHODSIAN, N TI SCREENING FOR MTDNA DIABETES MUTATIONS IN PIMA-INDIANS WITH NIDDM SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE MITOCHONDRIAL MUTATIONS; DIABETES MELLITUS; PIMA INDIANS ID MITOCHONDRIAL-DNA; MELLITUS; DEAFNESS; GENE; SUSCEPTIBILITY; TRANSMISSION; PREVALENCE; DELETION; OBESITY; LINKAGE AB More than half of the Pima Indians over age 35 years have non-insulin-dependent (type II) diabetes mellitus (NIDDM). Extensive data indicate the importance of maternal diabetes in determining their risk for diabetes. Generally, the risk of having NIDDM is higher in patients with affected mothers than affected fathers. This has been attributed to intrauterine factors, but recently mitochondrial inheritance has been raised as an alternative hypothesis. In other populations, several families and individuals with diabetes due to a mitochondrial DNA point mutation at nucleotide 3243 in the tRNA(leu(UUR)), gene have been described, as has one family with a 10.4 kb mitochondrial DNA duplication/deletion. We tested whether these specific mitochondrial gene mutations could explain a portion of the excess maternal transmission seen in the Pima Indians. Mitochondrial DNA obtained from blood lymphocytes of 148 Pima Indians with NIDDM was screened both for the point mutation at nt 3243, and the 10.4 kb duplication/deletion. Neither of these mutations was detected, and although a small proportion of the excess maternal transmission in Pima Indians could still be due to yet undescribed mitochondrial mutations or imprinted nuclear genes, our data support the role of the intrauterine environment in this population. (C) 1995 Wiley-Liss, Inc. C1 CEDARS SINAI RES INST,CTR MED GENET BIRTH DEFECTS,STEVEN SPIELBERG PEDIAT RES CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. NIDDKD,DIABET & ARTHRITIS EPIDEMIOL SECT,PHOENIX,AZ. FU NHLBI NIH HHS [HL 07386] NR 32 TC 13 Z9 14 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD MAR 27 PY 1995 VL 56 IS 2 BP 198 EP 202 DI 10.1002/ajmg.1320560217 PG 5 WC Genetics & Heredity SC Genetics & Heredity GA QQ365 UT WOS:A1995QQ36500016 PM 7625445 ER PT J AU PRIEL, E AFLALO, E SERI, I HENDERSON, LE ARTHUR, LO ABOUD, M SEGAL, S BLAIR, DG AF PRIEL, E AFLALO, E SERI, I HENDERSON, LE ARTHUR, LO ABOUD, M SEGAL, S BLAIR, DG TI DNA-BINDING PROPERTIES OF THE ZINC-BOUND AND ZINC-FREE HIV NUCLEOCAPSID PROTEIN - SUPERCOILED DNA UNWINDING AND DNA-PROTEIN CLEAVABLE COMPLEX-FORMATION SO FEBS LETTERS LA English DT Article DE HIV NUCLEOCAPSID PROTEIN; SUPERCOILED DNA; DNA-PROTEIN COMPLEX ID IMMUNODEFICIENCY-VIRUS TYPE-1; LEUKEMIA-VIRUS; TOPOISOMERASE-I; NUCLEIC-ACIDS; GENOMIC RNA; VIRAL-DNA; RETROVIRUSES; SEQUENCES; MUTATIONS; MUTANTS AB The HIV nucleocapsid (NC) protein contains, as those of other retroviruses, two Cys-His arrays which function as zinc finger binding domains. The nucleic acid binding properties of retroviral NC have been previously demonstrated. In this study, we characterized the DNA binding ability of the zinc-bound and zinc-free forms of HIV NC. We found that in addition to binding single-stranded DNA, both forms bind and unwind supercoiled plasmid DNA. The binding ability of the zinc-bound form was higher than the zinc-free form. In addition we showed the formation of NC protein-DNA cleavable complex which is the result of a presumably covalent bond formed between the protein and the phosphate moiety of the DNA backbone. The NC unwinding activity and the protein-DNA cleavable complex formation resembles the first step of the relaxing mechanism of DNA topoisomerase. Our results shed light on the possibility of a novel physiological function for the HIV NC protein in the viral life cycle. C1 NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,AIDS VACCINE PROGRAM,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,MOLEC ONCOL LAB,MICROBIOL SECT,FREDERICK,MD 21702. RP PRIEL, E (reprint author), BEN GURION UNIV NEGEV,FAC HLTH SCI,CANC RES CTR,DEPT IMMUNOL & MICROBIOL,BEER SHEVA,ISRAEL. NR 30 TC 14 Z9 14 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD MAR 27 PY 1995 VL 362 IS 1 BP 59 EP 64 DI 10.1016/0014-5793(95)00208-Q PG 6 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA QQ058 UT WOS:A1995QQ05800014 PM 7698354 ER PT J AU PU, LP HAYES, WP MILL, JF GHOSE, S FRIEDMAN, TC LOH, YP AF PU, LP HAYES, WP MILL, JF GHOSE, S FRIEDMAN, TC LOH, YP TI FROG PROHORMONE CONVERTASE PC2 MESSENGER-RNA HAS A MAMMALIAN-LIKE EXPRESSION PATTERN IN THE CENTRAL-NERVOUS-SYSTEM AND IS COLOCALIZED WITH A SUBSET OF THYROTROPIN-RELEASING HORMONE-EXPRESSING NEURONS SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE BRAIN; IN SITU HYBRIDIZATION HISTOCHEMISTRY; NEUROPEPTIDES; PROCESSING ENZYME; XENOPUS LAEVIS ID XENOPUS-LAEVIS; RANA-ESCULENTA; RAT-BRAIN; IMMUNOHISTOCHEMICAL LOCALIZATION; IMMUNOCYTOCHEMICAL EVIDENCE; PROPROTEIN CONVERTASES; PROCESSING PROTEINASES; MEDIAN-EMINENCE; MESSENGER-RNAS; CDNA STRUCTURE AB The prohormone convertase (PC2) is expressed in the mammalian central nervous system (CNS) and has been shown to play an important role in the processing of certain neuropeptide precursors and prohormones at paired basic residues. Amphibian PC2 cDNA was recently cloned for the frog Xenopus laevis, and both its sequence and its pituitary expression pattern were shown to be very similar to those of mammalian PC2. To investigate further the function of PC2 in the vertebrate CNS, we used in situ hybridization histochemistry to localize the distribution of cells expressing PC2 mRNA in the frog brain and the spinal cord. The distribution of PC2-expressing cells was also compared with that of cells expressing thyrotropin-releasing hormone (TRH) mRNA or peptide. PC2-expressing cells were detected in specific nuclei that were widely distributed in the frog CNS. In forebrain, telencephalic PC2 mRNA was found in the olfactory bulb, pallium, striatum, amygdala, and septum, and diencephalic PC2 mRNA was seen in the preoptic area, thalamus, and hypothalamus. More posteriorly, PC2 cells were localized to midbrain tegmentum, the torus semicircularis, and the optic tectum, as well as the cerebellum, brainstem, and spinal cord. Despite this wide distribution, steady-state levels of PC2 mRNA were clearly different in various brain nuclei. Regions with higher levels showed good correspondence to areas shown by others in frog to contain large numbers of neuropeptide expressing cells, including TRH cells. On the other hand, not all brain areas with high levels of TRH mRNA had high levels of PC2 mRNA. Localization studies combining in situ hybridization and immunocytochemistry showed that, at least in optic tectum and brainstem, PC2 mRNA and pro-TRH peptide coexist. These findings suggest that pro-TRH is processed by PC2 in some, but possibly not all, brain regions. Thus, different converting enzymes may be involved in pro-TRH processing in different brain regions. (C) 1995 Wiley-Liss, Inc.* C1 NICHHD,DEV NEUROBIOL LAB,CELLULAR NEUROBIOL SECT,BETHESDA,MD 20892. NINCDS,MOLEC BIOL LAB,BETHESDA,MD 20892. RI Ghose, Subroto/J-6732-2016 NR 65 TC 12 Z9 12 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD MAR 27 PY 1995 VL 354 IS 1 BP 71 EP 86 DI 10.1002/cne.903540107 PG 16 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA QL999 UT WOS:A1995QL99900006 PM 7615876 ER PT J AU DANIELPOUR, D ROBERTS, AB AF DANIELPOUR, D ROBERTS, AB TI SPECIFIC AND SENSITIVE QUANTITATION OF TRANSFORMING GROWTH-FACTOR BETA-3 BY SANDWICH ENZYME-LINKED-IMMUNOSORBENT-ASSAY SO JOURNAL OF IMMUNOLOGICAL METHODS LA English DT Article DE ELISA; TRANSFORMING GROWTH FACTOR BETA-3; IGY, CHICKEN; PURIFICATION; UMBILICAL CORD; (SERUM) ID DIFFERENTIAL REGULATION; EXPRESSION; IDENTIFICATION; FACTOR-BETA-2; PROMOTER; SEQUENCE; ACID AB Transforming growth factors beta (TGF-beta) consist of a highly homologous family of 25 kDa dimers involved in a diverse array of biological functions. Progress in understanding the biology of the third isoform (TGF-beta 3) of this family has been limited by the absence of a quantitative assay for TGF-beta 3. Here we report the development of a sensitive and specific sandwich enzyme-linked immunosorbent assay (SELISA), which is based on an anti-TGF-beta mouse monoclonal IgG as the capture antibody and chicken anti-recombinant-hTGF-beta 3 IgY as the secondary antibody. This assay can quantitate TGF-beta 3 in complex biological fluids, with a detection limit of 2 pg and no cross-reactivity or inteference with as high as 1000-fold molar excesses of either TGF-beta s 1, 2 or 1.2. This TGF-beta 3 SELISA is the first reported assay for the direct and sensitive quantitation of TGF-beta 3 in complex biological fluids. RP DANIELPOUR, D (reprint author), NCI,CHEMOPREVENT LAB,BLDG 41,ROOM C629,BETHESDA,MD 20892, USA. NR 19 TC 21 Z9 23 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1759 J9 J IMMUNOL METHODS JI J. Immunol. Methods PD MAR 27 PY 1995 VL 180 IS 2 BP 265 EP 272 DI 10.1016/0022-1759(94)00322-N PG 8 WC Biochemical Research Methods; Immunology SC Biochemistry & Molecular Biology; Immunology GA QQ910 UT WOS:A1995QQ91000012 PM 7714341 ER PT J AU FLEMING, K GOLDBERG, TE GOLD, JM WEINBERGER, DR AF FLEMING, K GOLDBERG, TE GOLD, JM WEINBERGER, DR TI VERBAL WORKING-MEMORY DYSFUNCTION IN SCHIZOPHRENIA - USE OF A BROWN-PETERSON PARADIGM SO PSYCHIATRY RESEARCH LA English DT Article DE NEUROPSYCHOLOGY; COGNITION; FRONTAL LOBE; MNEMONIC PERFORMANCE ID MONKEY PREFRONTAL CORTEX; DELAYED-RESPONSE TASK; CONNECTIONIST APPROACH; COGNITIVE DEFICIT; DOPAMINE; INTERFERENCE; INVOLVEMENT; REHEARSAL; DEMENTIA AB Recent studies of schizophrenia have implicated deficits in processes related to working memory, but the cognitive features of these deficits have been incompletely characterized. We used a modified Brown-Peterson paradigm to compare working memory in patients with schizophrenia and in normal control subjects. Distracter conditions differed in processing demand, increasing in complexity from no distracter to counting backwards (serial threes). We found significant effects of group, of distracter condition, and of a group x distracter condition interaction. The significant interaction was the result of a more rapid decline in the performance of schizophrenic patients with concurrent articulation. In addition, the schizophrenic group also made significantly more intrusion errors. The study suggests that schizophrenic patients exhibit dysfunction of the verbal working memory system due to a diminution in its overall processing resources. C1 NIMH,NEUROSCI CTR ST ELIZABETHS,WASHINGTON,DC 20032. NR 43 TC 85 Z9 85 U1 1 U2 3 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD MAR 27 PY 1995 VL 56 IS 2 BP 155 EP 161 DI 10.1016/0165-1781(95)02589-3 PG 7 WC Psychiatry SC Psychiatry GA RB753 UT WOS:A1995RB75300006 PM 7667440 ER PT J AU BAXEVANIS, AD BRYANT, SH LANDSMAN, D AF BAXEVANIS, AD BRYANT, SH LANDSMAN, D TI HOMOLOGY MODEL-BUILDING OF THE HMG-1 BOX STRUCTURAL DOMAIN SO NUCLEIC ACIDS RESEARCH LA English DT Article ID TESTIS-DETERMINING GENE; HISTONE CHROMOSOMAL-PROTEINS; TRANSCRIPTION FACTOR UBF; DNA-BINDING PROTEIN; MINOR-GROOVE; CRYSTAL-STRUCTURE; SEQUENCE; SRY; MOTIF; COMPLEX AB Nucleoproteins belonging to the HMG-1/-2 family possess homologous domains similar to 75 amino acids in length. These domains, termed HMG-1 boxes, are highly structured, compact, and mediate the interaction between HMG-1 box-containing proteins and DNA in a variety of biological contexts. Homology model building experiments on HMG-1 box sequences 'threaded' through the H-1-NMR structure of an HMG-1 box from rat indicate that the domain does not have rigid sequence requirements for its formation. Energy calculations indicate that the structure of all HMG-1 box domains is stabilized primarily through hydrophobic interactions. We have found structural relationships in the absence of statistically significant sequence similarity, identifying several candidate proteins which could possibly assume the same three-dimensional conformation as the rat HMG-1 box motif. The threading technique provides a method by which significant structural similarities in a diverse protein family can be efficiently detected, and the 'structural alignment' derived by this method provides a rational basis through which phylogenetic relationships and the precise sites of interaction between HMG-1 box proteins and DNA can be deduced. C1 NATL INST HLTH,NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20894. RI Landsman, David/C-5923-2009; OI Landsman, David/0000-0002-9819-6675 NR 57 TC 23 Z9 23 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD MAR 25 PY 1995 VL 23 IS 6 BP 1019 EP 1029 DI 10.1093/nar/23.6.1019 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QU682 UT WOS:A1995QU68200020 PM 7731789 ER PT J AU ABOUSSEKHRA, A BIGGERSTAFF, M SHIVJI, MKK VILPO, JA MONCOLLIN, V PODUST, VN PROTIC, M HUBSCHER, U EGLY, JM WOOD, RD AF ABOUSSEKHRA, A BIGGERSTAFF, M SHIVJI, MKK VILPO, JA MONCOLLIN, V PODUST, VN PROTIC, M HUBSCHER, U EGLY, JM WOOD, RD TI MAMMALIAN DNA NUCLEOTIDE EXCISION-REPAIR RECONSTITUTED WITH PURIFIED PROTEIN-COMPONENTS SO CELL LA English DT Article ID CELL NUCLEAR ANTIGEN; PIGMENTOSUM GROUP-F; BINDING-PROTEIN; DAMAGED DNA; REPLICATION PROTEIN; POLYMERASE-DELTA; TRANSCRIPTION FACTOR; PRIMATE CELLS; LIGASE-I; PURIFICATION AB Nucleotide excision repair is the principal way by which human cells remove UV damage from DNA. Human cell extracts were fractionated to locate active components, including xeroderma pigmentosum (XP) and ERCC factors. The incision reaction was then reconstituted with the purified proteins RPA, XPA, TFIIH (containing XPB and XPD), XPC, UV-DDB, XPG, partially purified ERCC1/XPF complex, and a factor designated IF7. UV-DDB (related to XPE protein) stimulated repair but was not essential. ERCC1- and XPF-correcting activity copurified with an ERCC1-binding polypeptide of 110 kDa that was absent in XP-F cell extract. Complete repair synthesis was achieved by combining these factors with DNA polymerase epsilon, RFC, PCNA, and DNA ligase I. The reconstituted core reaction requires about 30 polypeptides. C1 IMPERIAL CANC RES FUND,CLARE HALL LABS,S MIMMS EN6 3LD,HERTS,ENGLAND. INST GENET & BIOL MOLEC & CELLULAIRE,F-67404 ILLKIRCH GRAFFENS,FRANCE. UNIV ZURICH IRCHEL,DEPT VET BIOCHEM,CH-8057 ZURICH,SWITZERLAND. NICHHD,DNA REPLICAT REPAIR & MUTAGENESIS,BETHESDA,MD 20892. TAMPERE UNIV HOSP,DEPT CLIN CHEM,SF-33521 TAMPERE,FINLAND. RI Wood, Richard/E-7855-2011 OI Wood, Richard/0000-0002-9495-6892 NR 57 TC 678 Z9 692 U1 6 U2 19 PU CELL PRESS PI CAMBRIDGE PA 50 CHURCH ST CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD MAR 24 PY 1995 VL 80 IS 6 BP 859 EP 868 DI 10.1016/0092-8674(95)90289-9 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QP612 UT WOS:A1995QP61200005 PM 7697716 ER PT J AU JUSTICE, JM BLIZIOTES, MM STEVENS, LA MOSS, J VAUGHAN, M AF JUSTICE, JM BLIZIOTES, MM STEVENS, LA MOSS, J VAUGHAN, M TI INVOLVEMENT OF N-MYRISTOYLATION IN MONOCLONAL-ANTIBODY RECOGNITION SITES ON CHIMERIC G-PROTEIN ALPHA-SUBUNITS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID NUCLEOTIDE-BINDING-PROTEINS; ADP-RIBOSYLATION FACTOR; ROD OUTER SEGMENTS; BETA-GAMMA; ESCHERICHIA-COLI; PLASMA-MEMBRANE; AMINO TERMINUS; TRANSDUCIN; RHODOPSIN; RECONSTITUTION AB Monoclonal antibody, LAS-2, directed against the alpha subunit of transducin (G(t alpha)), inhibited G(t beta gamma-)dependent, pertussis toxin-catalyzed ADP ribosylation of G(t alpha) and was specific for G(t alpha). Immunoblotting studies on proteolytic fragments of G(t alpha) were consistent with an aminoterminal epitope. To define the antibody recognition site, recombinant G(t alpha) was synthesized in Escherichia coli cotransfected with or without yeast N-myristoyltransferase. Amino-terminal fatty acylation of G(t alpha), verified by use of radiolabeled fatty acid, was required for immunoreactivity. LAS-2 did not react with a chimeric protein consisting of residues 1-9 of G(t alpha) and the remainder G(o alpha), regardless of its myristoylation. Immunoreactivity was observed when amino acids 1-17 of G(t alpha) were present in a G(o alpha) chimera and the protein was amino-terminally myristoylated; there was no reactivity without myristoylation. It appears that the LAS-S epitope requires both G(t alpha)-specific sequence in amino acids 10-17 and a fatty acyl group in proximity to these residues. These results are consistent with the hypothesis that the myristoyl group is essential for protein structure; conceivably it ''folds back'' on and stabilizes the amino-terminal structure of G(t alpha), as opposed to protruding from an amino-terminal alpha-helix and serving as an amino terminal membrane anchor. RP JUSTICE, JM (reprint author), NHLBI,PULM CRIT CARE MED BRANCH,BLDG 10,RM 5N-307,10 CTR DR,MSC 1434,BETHESDA,MD 20892, USA. NR 38 TC 14 Z9 14 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 24 PY 1995 VL 270 IS 12 BP 6436 EP 6439 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ855 UT WOS:A1995QQ85500005 PM 7534763 ER PT J AU RYBA, NJP TIRINDELLI, R AF RYBA, NJP TIRINDELLI, R TI A NOVEL GTP-BINDING PROTEIN GAMMA-SUBUNIT, G-GAMMA-8, IS EXPRESSED DURING NEUROGENESIS IN THE OLFACTORY AND VOMERONASAL NEUROEPITHELIA SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ADRENERGIC-RECEPTOR KINASE; HETEROTRIMERIC G-PROTEINS; CENTRAL-NERVOUS-SYSTEM; BETA-GAMMA; SIGNAL TRANSDUCTION; MOLECULAR-CLONING; ADENYLYL CYCLASE; TYROSINE-PHOSPHATASE; ODORANT RECEPTORS; GENE-EXPRESSION AB A novel heterotrimeric G-protein gamma-subunit has been cloned, and its function has been confirmed by expression and purification. This gamma-subunit is only detected in the olfactory epithelium, the vomeronasal epithelium and, to a lesser extent, the olfactory bulb. It is absent from all other tissues studied including the nasal respiratory epithelium, During development, expression of G gamma 8 in the olfactory epithelium parallels neurogenesis, peaking shortly after birth and declining in the adult. In situ hybridization studies localize expression of this novel gamma-subunit to the sensory neurons; hybridization is strongest in the region of the epithelium that contains immature neurons, Unlike proteins that are expressed only in mature olfactory neurons (e.g. olfactory marker protein or Golf alpha), expression of G gamma 8 in the olfactory epithelium is relatively unaffected by olfactory bulbectomy. In the vomeronasal epithelium expression of G gamma 8 is also highest in the developing neurons. Taken together, these findings are consistent with a very specific role for G gamma 8 in the development and turnover of olfactory and vomeronasal neurons. C1 UNIV PARMA,I-43100 PARMA,ITALY. RP RYBA, NJP (reprint author), NIDR,IMMUNOL LAB,BLDG 10,RM 1A09,BETHESDA,MD 20892, USA. NR 60 TC 60 Z9 62 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 24 PY 1995 VL 270 IS 12 BP 6757 EP 6767 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ855 UT WOS:A1995QQ85500052 PM 7896821 ER PT J AU TAYLOR, ICA ROY, S YASWEN, P STAMPFER, MR VARMUS, HE AF TAYLOR, ICA ROY, S YASWEN, P STAMPFER, MR VARMUS, HE TI MOUSE MAMMARY-TUMORS EXPRESS ELEVATED LEVELS OF RNA-ENCODING THE MURINE HOMOLOG OF SKY, A PUTATIVE RECEPTOR TYROSINE KINASE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HUMAN-BREAST-CANCER; PP60C-SRC PROTEIN-KINASE; EPITHELIAL-CELL LINE; SIGNAL TRANSDUCTION; TRANSGENIC MICE; GROWTH-FACTOR; GENE; ONCOGENE; TRANSFORMATION; VIRUS AB To gain insight into the signal transduction pathways utilized by the Wnt-1-responsive mammary epithelial cell line C57MG, we screened for non-src family member tyrosine kinases expressed in these cells using a polymerase chain reaction-based technique. We identified five cDNA clones encoding receptor tyrosine kinases for which the ligand is known (fibroblast growth factor receptor, platelet-derived growth factor receptor, epithelial growth factor receptor, insulin receptor, and insulin-like growth factor receptor), two putative receptor tyrosine kinases for which the ligand remains to be identified (the products of ryk and the mouse klg homolog), and a novel tyrosine kinase. We cloned cDNAs encoding both the murine and human homologs of this kinase, the sequences of which were subsequently published under the names sky (Ohashi, K., Mizuno, K., Kuma, K., Miyata, T., and Nakamura, T. (1994) Oncogene 9, 699-705) and rse (Mark, M. R., Scadden, D. T., Wang, Z., Gu, Q., Goddard, A., and Godowski, P. J. (1994) J. Biol. Chem. 269, 10720-10728). Mouse sky RNA levels are abundant in mammary tumors derived from transgenic mice that express wnt-1, fgf-3, or both oncogenes in their mammary glands. However, little or no expression of sky is detected in mammary glands from virgin animals or in preneoplastic mammary glands from wnt-1 transgenic mice. Moreover, we find that the human homolog of sky is expressed at elevated levels when normal human mammary epithelial cells are rendered tumorigenic by the introduction of two viral oncogenes. Transient transfection of the human SKY cDNA into the quail fibrosarcoma cell line QT6 reveals that SKY is an active tyrosine kinase that augments the level of cellular phosphotyrosine. Introduction of murine Sky into RatB1a fibroblasts by retrovirus-mediated gene transfer results in morphological transformation, growth in soft agar, and the formation of tumors in nude mice. These data raise the possibility that the Sky tyrosine kinase is involved in the development and/or progression of mammary tumors. C1 NCI,BETHESDA,MD 20892. UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL,SAN FRANCISCO,CA 94143. UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,BERKELEY,CA 94720. FU NCI NIH HHS [CA-24844, CA-54247, CA-39832] NR 37 TC 43 Z9 44 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 24 PY 1995 VL 270 IS 12 BP 6872 EP 6880 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ855 UT WOS:A1995QQ85500066 PM 7896835 ER PT J AU ERNST, JA CLUBB, RT ZHOU, HX GRONENBORN, AM CLORE, GM AF ERNST, JA CLUBB, RT ZHOU, HX GRONENBORN, AM CLORE, GM TI DEMONSTRATION OF POSITIONALLY DISORDERED WATER WITHIN A PROTEIN HYDROPHOBIC CAVITY BY NMR SO SCIENCE LA English DT Article ID 3-DIMENSIONAL STRUCTURE; FOLDING THERMODYNAMICS; TRYPSIN-INHIBITOR; CRYSTAL-STRUCTURE; AQUEOUS-SOLUTION; INTERLEUKIN-1-BETA; HYDRATION; SPECTROSCOPY; RESOLUTION; MOLECULES AB The presence and location of water of hydration (thai is, bound water) in the solution structure of human interleukin-1 beta (hIL-1 beta) was investigated with water-selective two-dimensional heteronuclear magnetic resonance spectroscopy. It is shown here that in addition to water al the surface of the protein and ordered internal water molecules involved in bridging hydrogen bonds, positionally disordered water is present within a large, naturally occurring hydrophobic cavity located at the center oi the molecule. These water molecules of hydration have residency times in the range of 1 to 2 nanoseconds to 100 to 200 microseconds and can be readily detected by nuclear magnetic resonance (NMR). Thus, large hydrophobic cavities in proteins may not be truly empty, as analysis oi crystal structures appears to show, but may contain mobile water molecules that are crystallographically invisible but detectable by NMR. C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. RI Clore, G. Marius/A-3511-2008; Zhou, Huan-Xiang/M-5170-2016 OI Clore, G. Marius/0000-0003-3809-1027; Zhou, Huan-Xiang/0000-0001-9020-0302 NR 49 TC 202 Z9 205 U1 1 U2 16 PU AMER ASSOC ADVAN SCIENCE PI WASHINGTON PA 1333 H ST NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 24 PY 1995 VL 267 IS 5205 BP 1813 EP 1817 DI 10.1126/science.7892604 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QN702 UT WOS:A1995QN70200037 PM 7892604 ER PT J AU BOURASSA, MG ROUBIN, GS DETRE, KM SOPKO, G KRONE, RJ ATTABUTO, MJ BJERREGAAD, P BOLLING, S HERMAN, MV FRYE, R AF BOURASSA, MG ROUBIN, GS DETRE, KM SOPKO, G KRONE, RJ ATTABUTO, MJ BJERREGAAD, P BOLLING, S HERMAN, MV FRYE, R TI BYPASS ANGIOPLASTY REVASCULARIZATION INVESTIGATION - PATIENT SCREENING, SELECTION, AND RECRUITMENT SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article AB Percutaneous transluminal coronary angioplasty (PTCA) is currently performed in many patients seeking care because of severe manifestations of multivessel coronary artery disease. Previously, the majority of such patients would have undergone coronary artery bypass grafting (CABG). No definitive evidence is available as to which initial revascularization strategy has the best long-term clinical and economic outcomes. The Bypass Angioplasty Revascularization Investigation (BARI) is the largest of several recent clinical trials that were designed to test the hypothesis that an initial strategy of PTCA in selected patients with multivessel coronary artery disease does not compromise long-term clinical outcome compared with an initial strategy of CABG. This report describes how patients were screened, selected, and recruited in BARI and how this process may influence the results and the interpretation of the trial. During the enrollment period, 25,200 patients undergoing diagnostic coronary angiography at the participating institutions or with off-site angiograms referred to BARI investigators were screened for BARI eligibility. Excluded from screening were patients without coronary artery disease, those with single-vessel disease, prior revascularization, primary congenital, valvular, or myocardial disease, and age >80 years, Slightly more than half of the patients screened (12,670) were not clinically eligible for BARI because of left main disease, insufficient symptoms, emergency revascularization, or other logistic reasons. Thus, 12,530 patients had severe angina and/or ischemia and were clinically eligible for BARI. Nearly 33% of them (4,110) had multivessel disease, which was suitable for both PTCA and CABG. Of the 8,420 patients (67%) who were technically unsuitable, 60% were judged not to be candidates for PTCA, only 3% for CABG, and 3% for both procedures. Nearly half (1,829) of the patients who were clinically and angiographically eligible were randomly assigned to either PTCA (915) or CABG (914). The remaining 2,281 patients did not consent to randomization but 2,013 (88%) agreed to be followed in the eligible, not randomized registry. In addition, a random sample of 422 patients judged to be technically unsuitable for PTCA and/or CABG was enrolled in a registry of angiographic exclusions. Finally, to document ongoing revascularization practice patterns, the BARI investigators have provided semiannual 1-week surveys of all revascularizations at their respective centers as well as a 1-time survey at other institutions in the United States and Canada. Interpretation of the results of the BARI requires understanding the patient population entered into the study. Of the 25,200 patients with multivessel coronary artery disease age <80 years screened for the study, 12,670 were excluded for a variety of reasons (mostly left main disease or insufficient angina to warrant revascularization), and 8,420 were excluded because of technical unsuitability for both procedures. Of the eligible patients, 1,829 were entered into the study and randomized. Nonrandomized patients and a sample of ineligible patients were enrolled into registries. The data from the BARI registries and surveys will be important to put into perspective the relative long-term efficacy and safety of PTCA and CABG in patients who are suitable for both procedures. C1 UNIV ALABAMA,BIRMINGHAM,AL. UNIV PITTSBURGH,CTR COORDINATING,PITTSBURGH,PA. NHLBI,PROGRAM OFF,BETHESDA,MD 20892. JEWISH HOSP ST LOUIS,ST LOUIS,MO 63110. NYU,MED CTR,NEW YORK,NY. ST LOUIS UNIV,MED CTR,ST LOUIS,MO. UNIV MICHIGAN,MED CTR,ANN ARBOR,MI. NEW YORK MED COLL,VALHALLA,NY 10595. MAYO CLIN & MAYO FDN,OFF STUDY CHAIR,ROCHESTER,MN 55905. RP BOURASSA, MG (reprint author), MONTREAL HEART INST,5000 BELANGER ST E,MONTREAL,PQ H1T 1C8,CANADA. OI Bourassa, Martial G./0000-0002-4439-8650 NR 11 TC 78 Z9 81 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD MAR 23 PY 1995 VL 75 IS 9 BP C3 EP C8 DI 10.1016/S0002-9149(99)80389-0 PG 6 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QM422 UT WOS:A1995QM42200002 PM 7892820 ER PT J AU FRYE, RL KING, SB SOPKO, G DETRE, KM AF FRYE, RL KING, SB SOPKO, G DETRE, KM TI A SYMPOSIUM - MULTIVESSEL PTCA VERSUS CABG - BASE-LINE DATA FROM THE BYPASS ANGIOPLASTY REVASCULARIZATION INVESTIGATION (BARI) AND THE EMORY ANGIOPLASTY SURGERY TRIAL (EAST) - INTRODUCTION SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Editorial Material C1 EMORY UNIV HOSP,ATLANTA,GA 30322. UNIV PITTSBURGH,PITTSBURGH,PA. NHLBI,BETHESDA,MD 20892. RP FRYE, RL (reprint author), MAYO CLIN & MAYO FDN,ROCHESTER,MN 55906, USA. NR 0 TC 5 Z9 5 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD MAR 23 PY 1995 VL 75 IS 9 BP C1 EP C2 PG 2 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QM422 UT WOS:A1995QM42200001 ER PT J AU ROGERS, WJ ALDERMAN, EL CHAITMAN, BR DISCIASCIO, G HORAN, M LYTLE, B MOCK, MB ROSEN, AD SUTTONTYRRELL, K WEINER, BH WHITLOW, PL AF ROGERS, WJ ALDERMAN, EL CHAITMAN, BR DISCIASCIO, G HORAN, M LYTLE, B MOCK, MB ROSEN, AD SUTTONTYRRELL, K WEINER, BH WHITLOW, PL TI BYPASS ANGIOPLASTY REVASCULARIZATION INVESTIGATION (BARI) - BASE-LINE CLINICAL AND ANGIOGRAPHIC DATA SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article AB This report presents baseline clinical and angiographic data from the Bypass Angioplasty Revascularization Investigation (BARI), a multicenter international trial assessing the relative efficacy of percutaneous transluminal coronary angioplasty (PTCA) versus coronary artery bypass graft surgery (CABG) in selected patients with multivessel coronary artery disease. PTCA is commonly performed in patients with multivessel coronary artery disease, yet its long-term efficacy in comparison to CABG is unknown. From August 1988 through August 1991, 1,829 qualifying patients with multivessel disease suitable for either procedure were randomized to PTCA or CABG; sample size estimates were based on anticipated 5-year mortality. Two registry populations were also defined for follow-up: (1) 2,013 patients eligible for randomization but not randomized; and (2) 422 patients considered by angiography as unsuitable for randomization. Patients randomized in BARI were at relatively high risk for subsequent cardiac events: 39% were greater than or equal to 65 years old, 55% had prior myocardial infarction, 69% presented with unstable angina or non-Q wave myocardial infarction, and 43% had 3-vessel coronary artery disease. Patients randomized to PTCA and CABG were equally matched in all the important baseline variables. The randomized and the eligible but not randomized groups were similar in most respects. However, the nonrandomized group had a higher proportion with college education; fewer with a history of myocardial infarction, heart failure, diabetes, and smoking; and a somewhat better average ejection fraction. At the 3-month follow-up, PTCA had been performed more commonly in the nonrandomized eligible patients, especially those with 2-vessel disease. In comparison to the randomized patients, angiographically excluded patients were older; had more prior myocardial infarctions, heart failure, stable angina, complex coronary anatomy; and were less likely to undergo PTCA. The BARI randomized population is suitably composed to yield a meaningful comparison of the 5-year outcome of patients with multivessel disease eligible for either PTCA or CABG. The registry of eligible, nonrandomized patients and the registry of angiographically excluded patients should provide important ancillary data to complement the randomized trial. C1 STANFORD UNIV,MED CTR,STANFORD,CA 94305. VIRGINIA COMMONWEALTH UNIV MED COLL VIRGINIA,RICHMOND,VA. ST LOUIS UNIV,SCH MED,ST LOUIS,MO. NIH,BETHESDA,MD 20892. CLEVELAND CLIN FDN,CLEVELAND,OH 44195. MAYO CLIN & MAYO FDN,ROCHESTER,MN 55905. UNIV PITTSBURGH,PITTSBURGH,PA. UNIV MASSACHUSETTS,WORCESTER,MA 01605. RP ROGERS, WJ (reprint author), UNIV ALABAMA,MED CTR,334 LHR BLDG,BIRMINGHAM,AL 35294, USA. NR 11 TC 39 Z9 40 U1 0 U2 2 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD MAR 23 PY 1995 VL 75 IS 9 BP C9 EP C17 PG 9 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QM422 UT WOS:A1995QM42200003 PM 7892823 ER PT J AU SCHAFF, HV ROSEN, AD SHEMIN, RJ LECLERC, Y WAREING, TH AGUIRRE, FV SOPKO, G VANDERSALM, TJ LOOP, FD AF SCHAFF, HV ROSEN, AD SHEMIN, RJ LECLERC, Y WAREING, TH AGUIRRE, FV SOPKO, G VANDERSALM, TJ LOOP, FD TI CLINICAL AND OPERATIVE CHARACTERISTICS OF PATIENTS RANDOMIZED TO CORONARY-ARTERY BYPASS-SURGERY IN THE BYPASS ANGIOPLASTY REVASCULARIZATION INVESTIGATION (BARI) SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID VEIN-GRAFT PATENCY; INTERNAL-MAMMARY-ARTERY; CHRONIC STABLE ANGINA; MYOCARDIAL-INFARCTION; FOLLOW-UP; SURGICAL THERAPY; UNSTABLE ANGINA; NATIONAL-HEART; ANGIOGRAPHIC PREDICTORS; ANTIPLATELET THERAPY AB The surgical cohort of the Bypass Angioplasty Revascularization Investigation (BARI) is the largest group of patients with multivessel coronary artery disease randomly assigned to surgical treatment. This report presents baseline and operative characteristics of the cohort and describes some aspects of the variability in surgical practice among the 14 primary clinical centers and 4 so-investigational sites participating in BARI. Preoperative clinical and angiographic data and intraoperative variables were reviewed in 892 patients who were randomly assigned to coronary artery bypass grafting (CABG) and underwent operation. Associations between patient/lesion variables and operative characteristics are described. Of patients assigned to CABG, 87% underwent an operation within 2 weeks of randomization, as recommended in the protocol. Mean age of the 892 patients was 61 years, and mean age of the 235 women was greater than that of men (64 years vs 60 years); 64% of the surgical patients were classified as having unstable angina during the 6 weeks prior to randomization. Coronary angiography demonstrated 3-vessel disease (50% diameter narrowing by caliper measurement) in 41% of patients, and disease of the left anterior descending coronary artery was present in 87% of patients. A mean of 3.1 coronary arteries per patient were bypassed, and 82% of patients received 1 (70%) or 2 (12%) internal thoracic artery grafts. Prevalence of infernal thoracic grafts was lower in elderly patients (74% of patients greater than or equal to 70 years), in women (72% vs 85% in men; p < 0.01), and in black participants (65%). There was significant center-to-center variation in duration of cardiopulmonary bypass and aortic cross-clamping, methods of intraoperative myocardial protection, and in graft usage. Surgical patients in BARI differ considerably from patients entered into previous randomized trials in that the operative methods and graft usage reflect contemporary practice of coronary artery surgery, although significant variations among institutions were observed. C1 UNIV PITTSBURGH,CTR COORDINATING,PITTSBURGH,PA. BOSTON UNIV,MED CTR,BOSTON,MA. MONTREAL HEART INST,MONTREAL,PQ H1T 1C8,CANADA. JEWISH HOSP ST LOUIS,ST LOUIS,MO 63110. ST LOUIS UNIV,MED CTR,ST LOUIS,MO. NHLBI,BETHESDA,MD 20892. UNIV MASSACHUSETTS,WORCESTER,MA 01605. CLEVELAND CLIN FDN,CLEVELAND,OH 44195. RP SCHAFF, HV (reprint author), MAYO CLIN & MAYO FDN,200 1ST ST SW,ROCHESTER,MN 55905, USA. NR 73 TC 27 Z9 28 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD MAR 23 PY 1995 VL 75 IS 9 BP C18 EP C26 PG 9 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QM422 UT WOS:A1995QM42200004 PM 7892818 ER PT J AU WILLIAMS, DO BAIM, DS BATES, E BONAN, R BOST, JE COWLEY, M FAXON, DP FEIT, F JONES, R KELLETT, MA KELSEY, SF SOPKO, G STADIUS, M TOPOL, EJ AF WILLIAMS, DO BAIM, DS BATES, E BONAN, R BOST, JE COWLEY, M FAXON, DP FEIT, F JONES, R KELLETT, MA KELSEY, SF SOPKO, G STADIUS, M TOPOL, EJ TI CORONARY ANATOMIC AND PROCEDURAL CHARACTERISTICS OF PATIENTS RANDOMIZED TO CORONARY ANGIOPLASTY IN THE BYPASS ANGIOPLASTY REVASCULARIZATION INVESTIGATION (BARI) SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID ARTERY OCCLUSION AB The Bypass Angioplasty Revascularization Investigation (BARI) is a randomized multicenter clinical trial that compares a strategy of initial coronary angioplasty to initial coronary bypass surgery for patients with multivessel coronary artery disease. The purpose of this report is to describe the coronary anatomic characteristics of the 915 patients assigned to the angioplasty arm of the trial and the manner in which angioplasty was performed. Patients were eligible for BARI if they demonstrated multivessel coronary artery disease, had a clinical indication for revascularization, and were suitable for both coronary angioplasty and bypass surgery. Clinical and technical features of angioplasty procedures were systematically recorded. Coronary cineangiograms obtained before and during the angioplasty were interpreted by a central radiographic laboratory. Angioplasty was performed in 904 (98.8%) of the 915 patients assigned to that initial strategy. Of 6,530 coronary arterial lesions identified, 3,427 (52.5%) were significant (>50% diameter reduction). The majority of patients had 2-6 significant lesions, with 3 being most common. Angioplasty was attempted in 92.2% of the lesions for which it was intended. Lesions most frequently attempted ranged between 50% and 79% in severity. Multilesion angioplasty was performed in 77.5% of patients and 69.7% had multivessel angioplasty. Factors that influenced whether a lesion was attempted included lesion severity, clinical significance, and complexity. For lesions presenting as total occlusions, a history of recent infarction and postinfarction angina favored attempting angioplasty. Patients assigned to the angioplasty arm of BARI had evidence of extensive multilesion and multivessel coronary artery disease. An important component in performing angioplasty in such patients was lesion selection. Lesion morphology and the perceived clinical significance of lesions exerted the greatest influence on lesion selection. Observations of the manner in which angioplasty was performed in BARI provide insight into the contemporary application of angioplasty for patients with multivessel coronary artery disease. C1 BETH ISRAEL HOSP,BOSTON,MA 02215. MONTREAL HEART INST,MONTREAL,PQ H1T 1C8,CANADA. UNIV MICHIGAN,MED CTR,ANN ARBOR,MI. UNIV PITTSBURGH,CTR COORDINATING,PITTSBURGH,PA. VIRGINIA MED COLL,RICHMOND,VA. UNIV BOSTON HOSP,BOSTON,MA 02118. DUKE UNIV,MED CTR,DURHAM,NC. BELLEVUE HOSP CTR,NEW YORK,NY 10016. MAINE MED CTR,PORTLAND,ME 04102. NHLBI,BETHESDA,MD 20892. STANFORD UNIV,MED CTR,STANFORD,CA 94305. CLEVELAND CLIN FDN,CLEVELAND,OH 44195. RP WILLIAMS, DO (reprint author), BROWN UNIV,RHODE ISL HOSP,DIV CARDIOL,PROVIDENCE,RI 02903, USA. FU NHLBI NIH HHS [5 U01 HL 38532-07] NR 9 TC 19 Z9 22 U1 0 U2 1 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD MAR 23 PY 1995 VL 75 IS 9 BP C27 EP C33 PG 7 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QM422 UT WOS:A1995QM42200005 PM 7892819 ER PT J AU CROOP, CS AF CROOP, CS TI MUTATIONS IN BREAST-CANCER SO CANCER LETTERS LA English DT Article; Proceedings Paper CT 20th Meeting of the International-Association-for-Breast-Cancer-Research CY SEP 25-28, 1994 CL SENDAI, JAPAN SP INT ASSOC BREAST CANC RES DE BREASTS; CANCER; TUMOR SUPPRESSOR GENES; DELETIONS; BRCAI ID NM23 PROTEIN EXPRESSION; GOOD PROGNOSIS; GENE; CARCINOMAS; TUMORS; HETEROZYGOSITY; AMPLIFICATION; INT-2; MYC; CHROMOSOME-17Q21 AB The genetics of spontaneous breast cancer is reviewed. We have identified three regions of amplification and nine chromosomal arms with deletions in the genome. The significance and interrelations of these mutations is discussed with respect to the complex genetics of breast carcinoma. Recent work identifying a commonly deleted region between D17S846 and D17S746 is presented, which is approximately 0.5-1.0 Mb centromeric to the newly described BRCA1 gene candidate. Possible explanations for the different locations of our deleted region and the BRCA1 gene are presented. RP CROOP, CS (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,BLDG 10,BETHESDA,MD 20892, USA. NR 45 TC 0 Z9 0 U1 2 U2 3 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3835 J9 CANCER LETT JI Cancer Lett. PD MAR 23 PY 1995 VL 90 IS 1 BP 51 EP 56 PG 6 WC Oncology SC Oncology GA QU505 UT WOS:A1995QU50500008 ER PT J AU GERGEN, P MCQUILLAN, GM KIELY, M EZZATIRICE, TM SUTTER, RW VIRELLA, G AF GERGEN, P MCQUILLAN, GM KIELY, M EZZATIRICE, TM SUTTER, RW VIRELLA, G TI A POPULATION-BASED SEROLOGIC SURVEY OF IMMUNITY TO TETANUS IN THE UNITED-STATES SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID DIPHTHERIA IMMUNITY; VACCINATION; CHILDREN; REVACCINATION; IMMUNIZATION; ADULTS AB Background. Vaccination rates are frequently considered a surrogate measure of protection. To provide more accurate estimates, serum levels of antibody against tetanus were measured as part of the third National Health and Nutrition Examination Survey (NHANES III), which studied a representative sample of the civilian, noninstitutionalized population of the United States. Methods. We measured tetanus antitoxin using a solid-phase enzyme immunoassay in serum samples from 10,618 persons six years of age and older who were examined during phase 1 of NHANES III in 1988 to 1991. Results. Overall, 69.7 percent of Americans six years of age and older had protective levels of tetanus antibodies (>0.15 IU per milliliter). The rate decreased from 87.7 percent among those 6 to 11 years of age to 27.8 percent among those 70 years of age or older, Among children 6 to 16 years of age, 82.2 percent had protective levels of tetanus antibodies, with little variation according to race or ethnicity. More men than women were immune (79.0 percent vs. 62.4 percent). Mexican Americans had a significantly lower rate of immunity (57.9 percent, P<0.05) than either non-Hispanic whites (72.7 percent) or non-Hispanic blacks (68.1 percent). Those with a history of military service, higher levels of education, or incomes above the poverty level were more likely to have protective antibody levels. Although the prevalence of immunity declined rapidly starting at the age of 40 years, most of the 107 cases of tetanus (with 20 deaths) reported in 1989 and 1990 occurred in persons 60 years of age or older, Conclusions. Despite the fact that effective vaccines against tetanus have been available since the 1940s, many Americans do not have immunity to tetanus, and the rates are lowest among the elderly There is an excellent correlation between vaccination rates (96 percent) and immunity (96 percent) among six-year-olds. However, antibody levels decline over time, and one fifth of older children (10 to 16 years of age) do not have protective antibody levels. C1 NIAID,BETHESDA,MD 20892. CTR DIS CONTROL & PREVENT,NATL CTR HLTH STAT,HYATTSVILLE,MD 20782. US MATERNAL & CHILD HLTH BUR,ROCKVILLE,MD. CTR DIS CONTROL & PREVENT,NATL IMMUNIZATION PROGRAM,ATLANTA,GA 30333. MED UNIV S CAROLINA,DEPT MICROBIOL & IMMUNOL,CHARLESTON,SC 29425. NR 33 TC 208 Z9 211 U1 1 U2 4 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 23 PY 1995 VL 332 IS 12 BP 761 EP 766 DI 10.1056/NEJM199503233321201 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA QN442 UT WOS:A1995QN44200001 PM 7862178 ER PT J AU PERRIENS, JH STLOUIS, ME MUKADI, YB BROWN, C PRIGNOT, J POUTHIER, F PORTAELS, F WILLAME, JC MANDALA, JK KABOTO, M RYDER, RW ROSCIGNO, G PIOT, P AF PERRIENS, JH STLOUIS, ME MUKADI, YB BROWN, C PRIGNOT, J POUTHIER, F PORTAELS, F WILLAME, JC MANDALA, JK KABOTO, M RYDER, RW ROSCIGNO, G PIOT, P TI PULMONARY TUBERCULOSIS IN HIV-INFECTED PATIENTS IN ZAIRE - A CONTROLLED TRIAL OF TREATMENT FOR EITHER 6 OR 12 MONTHS SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID IMMUNODEFICIENCY-VIRUS INFECTION; HIV-1-INFECTED PATIENTS; CHEMOTHERAPY; MORTALITY; KINSHASA; KENYA AB Background. We studied the efficacy of a short-course regimen of chemotherapy for pulmonary tuberculosis in Kinshasa, Zaire. We also assessed whether, among patients with human immunodeficiency virus (HIV) infection, treatment should be extended from 6 to 12 months. Methods. HIV-seropositive and HIV-seronegative outpatients with pulmonary tuberculosis were treated with rifampin, isoniazid, pyrazinamide, and ethambutol daily for two months, followed by rifampin plus isoniazid twice weekly for four months. The HIV-positive patients who had no evidence of tuberculosis were then randomly assigned to receive either rifampin plus isoniazid or placebo twice weekly for a further six months. We also followed a comparison group of HIV-seronegative patients who received no further treatment for tuberculosis after six months. Results. After six months, 260 of 335 HIV-seropositive and 186 of 188 HIV-seronegative participants could be evaluated, and their rates of treatment failure were similar: 3.8 and 2.7 percent, respectively. At 24 months, the HIV-seropositive patients who received extended treatment had a relapse rate of 1.9 percent, as compared with 9 percent among the HIV-seropositive patients who received placebo for the second 6 months (P<0.01). Extended treatment did not improve survival, however. Among the HIV-seronegative patients, 5.3 percent relapsed. Conclusions. Among HIV-seropositive patients with pulmonary tuberculosis, extending treatment from 6 to 12 months reduces the rate of relapse but does not improve survival. The six-month program of partly intermittent antituberculous treatment may be an acceptable alternative when resources are limited. C1 PROJET SIDA,KINSHASA,ZAIRE. INST TROP MED,B-2000 ANTWERP,BELGIUM. BELGIAN AGCY DEV & COOPERAT,BRUSSELS,BELGIUM. CTR DIS CONTROL & PREVENT,DIV HIV & AIDS,ATLANTA,GA 30341. NIAID,BETHESDA,MD 20892. UNIV CATHOLIQUE LOUVAIN,MT GODINNE,BELGIUM. BUR NATL TB,KINSHASA,ZAIRE. CTR DEPISTAGE TB,KINSHASA,ZAIRE. NR 30 TC 225 Z9 229 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 23 PY 1995 VL 332 IS 12 BP 779 EP 784 DI 10.1056/NEJM199503233321204 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA QN442 UT WOS:A1995QN44200004 PM 7862181 ER PT J AU BARRETT, AJ POLLOCK, BH BUCHANAN, GR AF BARRETT, AJ POLLOCK, BH BUCHANAN, GR TI TREATMENT OF ACUTE LYMPHOBLASTIC-LEUKEMIA IN A 2ND REMISSION - REPLY SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 UNIV FLORIDA,COLL MED,GAINESVILLE,FL 32610. UNIV TEXAS,SW MED CTR,DALLAS,TX 75235. RP BARRETT, AJ (reprint author), NIH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 23 PY 1995 VL 332 IS 12 BP 824 EP 824 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA QN442 UT WOS:A1995QN44200028 ER PT J AU CARDENAS, AM KUIJPERS, GAJ POLLARD, HB AF CARDENAS, AM KUIJPERS, GAJ POLLARD, HB TI EFFECT OF PROTEIN-SYNTHESIS INHIBITORS ON SYNEXIN LEVELS AND SECRETORY RESPONSE IN BOVINE ADRENAL-MEDULLARY CHROMAFFIN CELLS SO BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES LA English DT Article DE CHROMAFFIN; SYNEXIN; PROTEIN SYNTHESIS; SECRETION; CYCLOHEXIMIDE; ACTINOMYCIN D ID MEMBRANE-FUSION; ANNEXIN-VII; CALCIUM; SYNAPTOTAGMIN; CYCLOHEXIMIDE; EXPRESSION; GRANULES; RELEASE AB The effects of the protein synthesis inhibitors actinomycin D and cycloheximide on the cellular content of the calcium binding protein synexin, and on the secretory response of cultured bovine adrenal medullary chromaffin cells were determined. Both protein synthesis inhibitors produced a slow decrease in the cellular synexin content. The synexin level was reduced by 50% after 133 h of incubation in the presence of 2 mu g/ml actinomycin D or 5 mu g/ml cycloheximide. However, this was partly due to an artefactual stabilization of synexin, since metabolic labelling of synexin with [S-35]methionine showed that the half-time of degradation was only 40 h. The secretory response of chromaffin cells was quickly diminished in the presence of protein synthesis inhibitors. Catecholamine secretion induced by membrane depolarization or barium stimulation of intact cells, or by calcium stimulation of digitonin-permeabilized cells was decreased by 77-82% after 24 h of incubation in the presence of 5 mu g/ml cycloheximide. These results suggest that, in addition to synexin, at least one or more proteins with a shorter half-time of degradation than synexin are involved in the secretory response of adrenal chromaffin cells. C1 NIDDK,CELL BIOL & GENET LAB,BETHESDA,MD 20892. NR 32 TC 8 Z9 8 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0005-2736 J9 BBA-BIOMEMBRANES JI Biochim. Biophys. Acta-Biomembr. PD MAR 22 PY 1995 VL 1234 IS 2 BP 255 EP 260 DI 10.1016/0005-2736(94)00283-U PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA QN667 UT WOS:A1995QN66700015 PM 7696302 ER PT J AU KHAN, AS TAYLOR, BR FILIE, JD RINGER, DP KOZAK, CA AF KHAN, AS TAYLOR, BR FILIE, JD RINGER, DP KOZAK, CA TI RAT PHENOL-PREFERRING SULFOTRANSFERASE GENES (STP AND STP2) - LOCALIZATION TO MOUSE CHROMOSOME-7 AND CHROMOSOME-17 SO GENOMICS LA English DT Note ID COMPLEMENTARY-DNA; N-HYDROXY-2-ACETYLAMINOFLUORENE; HEPATOCARCINOGENESIS; EXPRESSION; METABOLITE; IV AB The phenol-preferring sulfotransferases aryl sulfotransferase IV and N-hydroxyarylamine sulfotransferase catalyze sulfate conjugation of N-hydroxy-2-acetylaminofluorene, a metabolite capable of causing hepatocarcinogenesis in rats. We utilized published cDNA sequences of these sulfotransferases to type the progeny of two multilocus crosses and determined that the genes, aryl sulfotransferase (Stp) and N-hydroxyarylamine sulfotransferase (Stp2), map to positions on mouse chromosomes 7 and 17. (C) 1995 Academic Press, Inc. C1 NIH,BETHESDA,MD 20892. RP KHAN, AS (reprint author), OKLAHOMA MED RES FDN,NOBEL CTR BIOMED RES,825 NE 13TH ST,MAILSTOP 38,OKLAHOMA CITY,OK 73104, USA. NR 19 TC 3 Z9 3 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD MAR 20 PY 1995 VL 26 IS 2 BP 417 EP 419 DI 10.1016/0888-7543(95)80233-C PG 3 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA QR156 UT WOS:A1995QR15600036 PM 7601475 ER PT J AU SCIAKY, D JENKINS, NA GILBERT, DJ COPELAND, NG SONODA, G TESTA, JR COHEN, MB AF SCIAKY, D JENKINS, NA GILBERT, DJ COPELAND, NG SONODA, G TESTA, JR COHEN, MB TI MAPPING OF GUANYLIN TO MURINE CHROMOSOME-4 AND HUMAN-CHROMOSOME 1P34-P35 SO GENOMICS LA English DT Note ID FLUORESCENCE INSITU HYBRIDIZATION; ENDOGENOUS ACTIVATOR; DNA-SEQUENCES; MESSENGER-RNA; RAT COLON; MOUSE; CYCLASE; LOCALIZATION; CDNA C1 CHILDRENS HOSP,MED CTR,DIV PEDIAT GASTROENTEROL & NUTR,CINCINNATI,OH 45229. UNIV CINCINNATI,CINCINNATI,OH. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,FREDERICK,MD. FOX CHASE CANC CTR,DEPT MED ONCOL,PHILADELPHIA,PA 19111. OI Cohen, Mitchell/0000-0002-4412-350X FU NCI NIH HHS [CA-06927, N01-CO-74101, CA-45745] NR 20 TC 16 Z9 17 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD MAR 20 PY 1995 VL 26 IS 2 BP 427 EP 429 DI 10.1016/0888-7543(95)80238-H PG 3 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA QR156 UT WOS:A1995QR15600041 PM 7601480 ER PT J AU SUKHOV, RR WALKER, LC RANCE, NE PRICE, DL YOUNG, WS AF SUKHOV, RR WALKER, LC RANCE, NE PRICE, DL YOUNG, WS TI OPIOID PRECURSOR GENE-EXPRESSION IN THE HUMAN HYPOTHALAMUS SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE BASAL FOREBRAIN; DYNORPHIN; ENKEPHALIN; INTERMEDIATE NUCLEUS; PROOPIOMELANOCORTIN ID SEXUALLY DIMORPHIC NUCLEUS; MESSENGER-RIBONUCLEIC-ACID; HYPOPHYSEAL PORTAL BLOOD; CENTRAL NERVOUS-SYSTEM; RHESUS-MONKEY BRAIN; LUTEINIZING-HORMONE SECRETION; MEDIAL BASAL HYPOTHALAMUS; BETA-NEO-ENDORPHIN; RAT-BRAIN; INSITU HYBRIDIZATION AB Using in situ hybridization histochemistry, we studied the distribution of neurons that express preproopiomelanocortin (pre-POMC), preprodynorphin (pre-PDYN), and preproenkephalin (pre-PENK) gene transcripts within the human hypothalamus and surrounding structures. Of the three opioid systems, pre-POMC neurons have the most restricted distribution. Pre-POMC cells are most numerous in the infundibular nucleus and retrochiasmatic area of the mediobasal hypothalamus; a few labeled cells are present within the boundaries of the ventromedial nucleus and infundibular stalk. Pre-POMC message was not found in the limited samples of structures adjacent to the hypothalamus. In contrast to neurons that express pre-POMC, neurons expressing pre-PDYN and pre-PENK are more widely represented throughout the hypothalamus and extrahypothalamic structures. However, pre-PDYN and pre-PENK cells differ from one another in distribution. Pre-PDYN message is especially abundant in neurons of the tuberal and mammillary regions, with a distinct population of labeled cells in the premammillary nucleus and dorsal posterior hypothalamus. Pre-PDYN gene expression also is found in neurons of the dorsomedial nucleus, ventromedial nucleus, caudal magnocellular portion of the paraventricular nucleus, dorsolateral supraoptic nucleus, tuberomammillary nucleus, caudal lateral hypothalamus, and retrochiasmatic area. In structures immediately adjacent to the hypothalamus, pre-PDYN neurons were observed in the caudate nucleus, putamen, cortical nucleus of the amygdala, and bed nucleus of the stria terminalis. Pre-PENK neurons occur in varying numbers in all hypothalamic nuclei except the mammillary bodies. The chiasmatic region is particularly rich in pre-PENK neurons, with the highest packing density in the intermediate nucleus [the intermediate nucleus (Braak and Braak [1987] Anat. Embryol. 176:315-330) has also been termed the sexually dimorphic nucleus of the preoptic area (SDA-POA; Swaab and Fliers [1985] Science 228:1112-1115) or the interstitial nucleus of the anterior hypothalamus 1 (Allen et al. [1989] J. Neurosci. 9:497-506)], dorsal suprachiasmatic nucleus, medial preoptic area, and rostral lateral hypothalamic area. Pre-PENK neurons are numerous in the infundibular nucleus, ventromedial nucleus, dorsomedial nucleus, caudal parvicellular portion of the paraventricular nucleus, tuberomammillary nucleus, lateral hypothalamus, and retrochiasmatic area. Only a few lightly labeled cells were found in the periphery of the supraoptic nucleus and lateral tuberal nucleus. In areas adjacent to the hypothalamus, cells that contain pre-PENK message occur in the nucleus basalis of Meynert, central nucleus of amygdala, bed nucleus of the stria terminalis, caudate nucleus, and putamen. The differential distribution of pre-POMC, pre-PDYN, and pre-PENK neurons in the human hypothalamus suggests that these three opioid systems influence hypothalamic functions in quite different ways. (C) 1995 Wiley-Liss, Inc. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT PATHOL,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,DEPT PSYCHOL,BALTIMORE,MD 21218. UNIV ARIZONA,COLL MED,DEPT PATHOL,TUCSON,AZ 85724. UNIV ARIZONA,COLL MED,DEPT NEUROL,TUCSON,AZ 85724. UNIV ARIZONA,COLL MED,DEPT ANAT,TUCSON,AZ 85724. NIMH,CELL BIOL LAB,BETHESDA,MD 20892. RP SUKHOV, RR (reprint author), JOHNS HOPKINS UNIV,SCH MED,NEUROPATHOL LAB,558 ROSS RES BLDG,720 RUTLAND AVE,BALTIMORE,MD 21205, USA. RI Young, W Scott/A-9333-2009; Walker, L/J-6541-2015 OI Young, W Scott/0000-0001-6614-5112; Walker, L/0000-0001-9166-3261 FU NINDS NIH HHS [NS 20471, NS AG 05146, NS07179] NR 141 TC 35 Z9 36 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD MAR 20 PY 1995 VL 353 IS 4 BP 604 EP 622 DI 10.1002/cne.903530410 PG 19 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA QJ296 UT WOS:A1995QJ29600009 PM 7759618 ER PT J AU PANTALEO, G EMBRETSON, J AF PANTALEO, G EMBRETSON, J TI HOT PAPERS - AIDS RESEARCH - MASSIVE COVERT INFECTION OF HELPER T-LYMPHOCYTES AND MICROPHAGES BY HIV DURING INCUBATION PERIOD OF AIDS BY EMBRETSON,J., ZUPANCIC,M., RIBAS,J.L., BURKE,A., RACZ,P., TENNERRACZ,K., HAASE,A.T. SO SCIENTIST LA English DT Editorial Material C1 SCHWEGMAN LUNDBERG & WOESSNER PA,MINNEAPOLIS,MN. RP PANTALEO, G (reprint author), NIAID,BETHESDA,MD 20892, USA. RI Pantaleo, Giuseppe/K-6163-2016 NR 1 TC 0 Z9 0 U1 0 U2 2 PU SCIENTIST INC PI PHILADELPHIA PA 3600 MARKET ST SUITE 450, PHILADELPHIA, PA 19104 SN 0890-3670 J9 SCIENTIST JI Scientist PD MAR 20 PY 1995 VL 9 IS 6 BP 15 EP 15 PG 1 WC Information Science & Library Science; Multidisciplinary Sciences SC Information Science & Library Science; Science & Technology - Other Topics GA QM423 UT WOS:A1995QM42300016 ER PT J AU PANTALEO, G EMBRESTSON, J AF PANTALEO, G EMBRESTSON, J TI HOT PAPERS - AIDS RESEARCH - HIV-INFECTION IS ACTIVE AND PROGRESSIVE IN LYMPHOID-TISSUE DURING THE CLINICALLY LATENT STAGE OF DISEASE BY PANTALEO,G., GRAZIOSI,C., DEMAREST,J.M., BUTINI,L., MONTRONI,M., FOX,C.H., ORENSTEIN,J.M., KOTLER,D.P., FAUCI,A.S. SO SCIENTIST LA English DT Editorial Material C1 SCHWEGMAN LUNDBERG & WOESSNER PA,MINNEAPOLIS,MN. RP PANTALEO, G (reprint author), NIAID,BETHESDA,MD 20892, USA. RI Pantaleo, Giuseppe/K-6163-2016 NR 1 TC 0 Z9 0 U1 0 U2 2 PU SCIENTIST INC PI PHILADELPHIA PA 3600 MARKET ST SUITE 450, PHILADELPHIA, PA 19104 SN 0890-3670 J9 SCIENTIST JI Scientist PD MAR 20 PY 1995 VL 9 IS 6 BP 15 EP 15 PG 1 WC Information Science & Library Science; Multidisciplinary Sciences SC Information Science & Library Science; Science & Technology - Other Topics GA QM423 UT WOS:A1995QM42300015 ER PT J AU ROMANOV, VI WRATHALL, LS SIMMONS, TD DASILVA, PP SOBEL, ME AF ROMANOV, VI WRATHALL, LS SIMMONS, TD DASILVA, PP SOBEL, ME TI PROTEIN-SYNTHESIS IS REQUIRED FOR LAMININ-INDUCED EXPRESSION OF THE 67-KDA LAMININ RECEPTOR AND ITS 37-KDA PRECURSOR SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID TUMOR INVASION; METASTASIS AB The high affinity 67-kDa laminin receptor (67LR) is highly expressed in metastatically active human cancers. A 37 - kDa polypeptide has been identified as its precursor (37LRP). Antibodies raised against 37LRP-derived synthetic peptides were used in immunogold electron microscopy and immunoblot studies to assess the effect of laminin on expression of the 67LR and the 37LRP. Laminin (15 mu g/ml) treatment of suspended A2058 human melanoma cells doubled the expression of both 37LRP and the 67LR. Fibronectin had no effect. There was no effect of laminin on the expression of actin or galectin-3. Cycloheximide treatment. of cells prior to laminin abrogated its inducible effect. The results suggest that binding of laminin by cell surface laminin receptors induces synthesis of the 37LRP and mature 67LR, with a consequent delivery to the cell surface of more laminin binding proteins for potentiated attachment of the melanoma cell to the basement membrane during invasion and metastasis. C1 NCI,MOLEC PATHOL SECT,BETHESDA,MD 20892. NCI,FREDERICK CANC RES & DEV CTR,MEMBRANE BIOL SECT,FREDERICK,MD 21702. NR 12 TC 20 Z9 20 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD MAR 17 PY 1995 VL 208 IS 2 BP 637 EP 643 DI 10.1006/bbrc.1995.1386 PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA QM952 UT WOS:A1995QM95200027 PM 7695618 ER PT J AU ROSENBERG, HF MORRISON, RP LI, F AF ROSENBERG, HF MORRISON, RP LI, F TI IDENTIFICATION OF A 40-KDA HUMAN PROTEIN THAT CROSS-REACTS WITH PROKARYOTIC HSP60 CHAPERONINS SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID HEAT-SHOCK PROTEIN; RHEUMATOID-ARTHRITIS; ESCHERICHIA-COLI; STRESS PROTEINS; MYCOBACTERIUM-TUBERCULOSIS; NUCLEOTIDE-SEQUENCE; ANTIBODY-LEVELS; GENE; ANTIGEN; CELL AB Cross-reactivity between the immunodominant bacterial hsp60 proteins and heterologous human target proteins has led to numerous hypotheses on the role of hsp60 in the pathogenesis of autoimmune disease. In this work, we describe a novel 40-kDa human protein that crossreacts with bacterial hsp60 proteins. CCP40 (chaperone cross-reacting protein, 40-kDa) was identified in extracts from HL-60 (human promyelocytic leukemia) cells on Western blots probed with A57-E4, a monoclonal antibody specifying a linear polypeptide epitope common among the bacterial hsp60 proteins (Yuan et. al. (1992) Inf. Immun. 60, 2288-2296). CCP40 was detected in other human hematopoietic cell lines, but could not be detected in mature peripheral blood leukocytes. CCP40 was also expressed in human CD34+ peripheral blood progenitor cells, disappearing with cytokine-induced cellular maturation. (C) 1995 Academic Press. Inc. C1 NIAID,ROCKY MT LABS,INTRACELLULAR PARASITES LAB,HAMILTON,MT 59840. RP ROSENBERG, HF (reprint author), NIAID,HOST DEF LAB,BETHESDA,MD 20892, USA. NR 34 TC 1 Z9 1 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD MAR 17 PY 1995 VL 208 IS 2 BP 697 EP 703 DI 10.1006/bbrc.1995.1394 PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA QM952 UT WOS:A1995QM95200035 PM 7695625 ER PT J AU AHMED, AH JACOBSON, KA KIM, JH HEPPEL, LA AF AHMED, AH JACOBSON, KA KIM, JH HEPPEL, LA TI PRESENCE OF BOTH A(1) AND A(2A) ADENOSINE RECEPTORS IN HUMAN-CELLS AND THEIR INTERACTION SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID BETA-GAMMA-SUBUNITS; ADENYLYL CYCLASE; PERTUSSIS TOXIN; DNA-SYNTHESIS; RAT-BRAIN; PROTEIN; STIMULATION; BINDING AB We have obtained pharmacological evidence for the expression of both A(1)- (inhibitory) and A(2a)- (stimulatory) adenosine receptors in cultured human foreskin fibroblasts and lung fibroblasts. The Al receptors were sufficiently abundant in foreskin fibroblasts so that binding studies with a radioactively labeled specific ligand confirmed their existence. Both receptors were activated during the stimulation of cAMP accumulation and DNA synthesis by adenosine. (C) 1995 Academic Press, Inc. C1 NIDDK,CHEM LAB,BETHESDA,MD 20892. RP AHMED, AH (reprint author), CORNELL UNIV,BIOCHEM MOLEC & CELL BIOL SECT,ITHACA,NY 14853, USA. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z01 DK031117-20, Z99 DK999999]; NCI NIH HHS [5 RO1 CA58518] NR 17 TC 13 Z9 13 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD MAR 17 PY 1995 VL 208 IS 2 BP 871 EP 878 DI 10.1006/bbrc.1995.1416 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA QM952 UT WOS:A1995QM95200057 PM 7695645 ER PT J AU ZHANG, LX MILLS, KJ DAWSON, MI COLLINS, SJ JETTEN, AM AF ZHANG, LX MILLS, KJ DAWSON, MI COLLINS, SJ JETTEN, AM TI EVIDENCE FOR THE INVOLVEMENT OF RETINOIC ACID RECEPTOR RAR-ALPHA-DEPENDENT SIGNALING PATHWAY IN THE INDUCTION OF TISSUE TRANSGLUTAMINASE AND APOPTOSIS BY RETINOIDS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HUMAN NEUROBLASTOMA-CELLS; LUNG-CARCINOMA CELLS; GENE-EXPRESSION; NUCLEAR RECEPTORS; EPIDERMAL-CELLS; HL-60 CELLS; DEATH; RXR; DIFFERENTIATION; IDENTIFICATION AB In this study, we show that all-trans-retinoic acid (RA) is a potent inducer of tissue transglutaminase (TGase II) and apoptosis in the rat tracheobronchial epithelial cell line SPOC-1. We demonstrate that these cells express the retinoid receptors RAR alpha, RAR gamma, and RXR beta. To identify which of these receptors are involved in regulating these processes, we analyzed the effects of several receptor-selective agonists, an antagonist, and a dominant-negative RAR alpha. We show that the RAR-selective retinoid SRI-6751-84 strongly increased TGase II expression at both the protein and mRNA levels, whereas the RXR-selective retinoid SR11217 had little effect. The RAR alpha-selective retinoid Ro40-6055 was also able to induce TGase II, whereas the RAR gamma-selective retinoid CD437 was inactive. The induction of TGase II by the RAR-selective retinoid was completely inhibited by the RAR alpha-antagonist Ro41-5253. Overexpression of a truncated RAR alpha gene with dominant-negative activity also inhibited the induction of TGase II expression. The increase in TGase II is associated with an induction of apoptosis as revealed by DNA fragmentation and the generation of apoptotic cells. We demonstrate that apoptosis is affected by retinoids in a manner similar to TGase II. Our results suggest that the induction of TGase II expression and apoptosis in SPOC-1 cells are mediated through an RAR alpha-dependent signaling pathway. C1 NIEHS,PULM PATHOBIOL LAB,CELL BIOL SECT,RES TRIANGLE PK,NC 27709. SRI INT,BIOORGAN CHEM LAB,MENLO PK,CA 94025. FRED HUTCHINSON CANC RES CTR,PROGRAM MOLEC MED,SEATTLE,WA 98104. OI Jetten, Anton/0000-0003-0954-4445 FU NCI NIH HHS [CA-55397] NR 54 TC 128 Z9 132 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 17 PY 1995 VL 270 IS 11 BP 6022 EP 6029 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QM945 UT WOS:A1995QM94500049 PM 7890733 ER PT J AU HUH, HY LO, SK YESNER, LM SILVERSTEIN, RL AF HUH, HY LO, SK YESNER, LM SILVERSTEIN, RL TI CD36 INDUCTION ON HUMAN MONOCYTES UPON ADHESION TO TUMOR NECROSIS FACTOR-ACTIVATED ENDOTHELIAL-CELLS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID GENE-EXPRESSION; GLYCOPROTEIN-IV; MELANOMA-CELLS; GROWTH-FACTOR; RECEPTOR; IDENTIFICATION; SPECIFICITY; RECOGNITION; NEUTROPHILS; BINDING AB Cell adhesion between circulating monocytes and the endothelium is a critical component of vascular thromboregulation and atherogenesis. The biochemical and genetic consequences of adhesion are poorly understood, We have found that monocyte surface expression of CD36, an integral membrane receptor for thrombospondin, collagen, and oxidized low density lipoprotein, increased dramatically upon adhesion to tumor necrosis factor-activated human umbilical vein endothelial cells (HUVEC), Expression was assessed by indirect immunofluorescence microscopy and immunoblotting using monoclonal antibodies to CD36, Steady-state CD36 mRNA levels, detected by RNase protection assay, also showed a similar pattern of up-regulation, To verify the adhesion dependence of the observed phenomenon, monocytes were co-cultured with tumor necrosis factor-activated HUVEC in a transwell apparatus that physically separated monocytes from the endothelial cells, Under these conditions, no increase in CD36 expression was detected, demonstrating that the enhanced monocyte CD36 expression observed is not due to soluble factors released by HUVEC, To characterize the specific adhesion molecules involved in the process, co culture assays were performed on murine L cells transfected with either human E-selectin or intercellular adhesion molecule-1 cDNAs, A dramatic increase in CD36 mRNA was seen upon monocyte adhesion to E-selectin-transfected L cells compared with adhesion to intercellular adhesion molecule-1 or control transfectants, Furthermore, monoclonal antibodies to E-selectin inhibited the adhesion-dependent up-regulation of CD36 mRNA induced by transfected L cells or cytokine-activated endothelial cells, These findings demonstrate adhesion-dependent gene regulation of monocyte CD36 and suggest the possible involvement of E-selectin in initiating this process. C1 CORNELL UNIV,COLL MED,DEPT MED,DIV HEMATOL & ONCOL,NEW YORK,NY 10021. CORNELL UNIV,COLL MED,CELL BIOL & GENET PROGRAM,NEW YORK,NY 10021. CORNELL UNIV,COLL MED,NATL INST HLTH,SPECIALIZED CTR RES THROMBOSIS,NEW YORK,NY 10021. FU NHLBI NIH HHS [HL 18828, HL 46403, HL 42540] NR 22 TC 46 Z9 47 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 17 PY 1995 VL 270 IS 11 BP 6267 EP 6271 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QM945 UT WOS:A1995QM94500083 PM 7534309 ER PT J AU RUVINOV, SB YANG, XJ PARRIS, KD BANIK, U AHMED, SA MILES, EW SACKETT, DL AF RUVINOV, SB YANG, XJ PARRIS, KD BANIK, U AHMED, SA MILES, EW SACKETT, DL TI LIGAND-MEDIATED CHANGES IN THE TRYPTOPHAN SYNTHASE INDOLE TUNNEL PROBED BY NILE-RED FLUORESCENCE WITH WILD-TYPE, MUTANT, AND CHEMICALLY-MODIFIED ENZYMES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID SITE-SPECIFIC MUTAGENESIS; ESCHERICHIA-COLI; ALPHA-SUBUNIT; BETA-SUBUNIT; SALMONELLA-TYPHIMURIUM; BIENZYME COMPLEX; ALPHA-2-BETA-2 COMPLEX; FLEXIBLE LOOP; CONFORMATIONAL-CHANGES; ENZYMATIC-ACTIVITIES AB The bacterial tryptophan synthase alpha(2) beta(2) complex contains an unusual structural feature: an intramolecular tunnel that channels indole from the active site of the alpha subunit to the active site of the beta subunit 25 Angstrom A away, Here we investigate the role of the tunnel in communication between the alpha and beta subunits using the polarity-sensitive fluorescent probe, Nile Red. Interaction of Nile Red in the nonpolar tunnel near beta subunit residues Cys-170 and Phe-280 is supported by studies with enzymes altered at these positions. Restricting the tunnel by enlarging Cys-170 by chemical modification or mutagenesis decreases the fluorescence of Nile Red by 30-70%. Removal of a partial restriction in the tunnel by replacing Phe-280 by Cys or Ser increases the fluorescence of Nile Red more than 2-fold. A binding site for Nile Red in this region near the pyridoxal phosphate coenzyme of the beta subunit is further supported by iodide quenching and fluorescence energy transfer experiments and by molecular modeling based on the three-dimensional structure of the alpha(2) beta(2) complex. Finally, studies using Nile Red as a sensitive probe of conformational changes in the tunnel reveal that allosteric ligands (alpha subunit) or active site ligands (beta subunit) decrease the fluorescence of Nile Red. We speculate that allosteric and active site ligands induce a tunnel restriction near Phe-280 that serves as a gate to control passage of indole through the tunnel. C1 NIH, NIDDK, BIOCHEM PHARMACOL LAB, BETHESDA, MD 20892 USA. NIH, NIDDK, MOLEC BIOL LAB, BETHESDA, MD 20892 USA. NR 63 TC 51 Z9 52 U1 0 U2 4 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 17 PY 1995 VL 270 IS 11 BP 6357 EP 6369 PG 13 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QM945 UT WOS:A1995QM94500096 PM 7890774 ER PT J AU BONNER, JC BADGETT, A HOFFMAN, M LINDROOS, PM AF BONNER, JC BADGETT, A HOFFMAN, M LINDROOS, PM TI INHIBITION OF PLATELET-DERIVED GROWTH FACTOR-BB-INDUCED FIBROBLAST PROLIFERATION BY PLASMIN-ACTIVATED ALPHA(2)-MACROGLOBULIN IS MEDIATED VIA AN ALPHA(2)-MACROGLOBULIN RECEPTOR LOW-DENSITY-LIPOPROTEIN RECEPTOR-RELATED PROTEIN-DEPENDENT MECHANISM SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID IDIOPATHIC PULMONARY FIBROSIS; RAT LUNG FIBROBLASTS; SMOOTH-MUSCLE CELLS; ALPHA-2-MACROGLOBULIN RECEPTOR; ALPHA-MACROGLOBULIN; FACTOR PDGF; ALVEOLAR MACROPHAGES; CYTOKINE BINDING; GENE-EXPRESSION; FACTOR-BETA AB alpha(2)-Macroglobulin (alpha(2)M) is a potentially important regulator of platelet-derived growth factor-BB (PDGF-BB)stimulated cell growth due to our previous observation that PDGF-BB binds to alpha(2)M noncovalently (Bonner, J. C., Goodell, A. L., Lasky, J. A., and Hoffman, M. R. (1992) J. Biol. Chem. 267, 12837-12844). We examined the in vitro effect of native and plasmin activated (receptor-recognized) alpha(2)M on the PDGF-BB-induced proliferation of mouse Swiss 3T3 and rat lung fibroblasts. Nondenaturing polyacrylamide gel electrophoresis showed that plasmin converted alpha(2)M to its electrophoretically ''fast'' form at a 2:1 molar ratio and that I-125-PDGF-BB bound both alpha(2)M and alpha(2)M-plasmin. PDGF-BB-induced growth was not affected by native alpha(2)M (0.3 mu M) or plasmin (0.6 mu M). The combination of plasmin and alpha(2)M (2:1 molar ratio) inhibited PDGF-BB induced cell proliferation 80-90%. Complexes of PDGF-BB .alpha(2)M purified by gel filtra tion chromatography retained growth promoting activity, but the PDGF-BB .alpha(2)M-plasmin complex did not, Preincubation of fibroblasts (37 degrees C for 24 h) with alpha(2)M-plasmin did not change I-125-PDGF BB binding or affect gene expression of the 6.5 kilobase PDGF-alpha receptor or 5.2-kilobase PDGF-beta receptor mRNA However, preincubation with alpha(2)M-plasmin (0-4 degrees C for 4 h) increased I-125-PDGF-BB binding a fold, and this increase was blocked by a receptor-associated protein antagonist of the alpha(2)M-receptor/low density lipoprotein receptor-related protein, The receptor-associated protein antagonist blocked I-125-alpha(2)M-methylamine binding, inhibited PDGF-BB-alpha(2)M-plasmin uptake from fibroblast-cultured supernatants, and abolished the inhibitory effect of alpha(2)M-plasmin on PDGF-stimulated growth, These data suggest that inhibition of PDGF-stimulated proliferation by alpha(2)M-plasmin is mediated in part by clearance of PDGF-BB-alpha(2)M-plasmin through the lipoprotein receptor-related protein. C1 DUKE UNIV,MED CTR,DURHAM VET AFFAIRS MED CTR,DEPT PATHOL,SERV LAB,DURHAM,NC 27705. RP BONNER, JC (reprint author), NIEHS,PULM PATHOBIOL LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 60 TC 24 Z9 24 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 17 PY 1995 VL 270 IS 11 BP 6389 EP 6395 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QM945 UT WOS:A1995QM94500099 PM 7534312 ER PT J AU XU, ZQ QIU, YL CHOKEKIJCHAI, S MITSUYA, H ZEMLICKA, J AF XU, ZQ QIU, YL CHOKEKIJCHAI, S MITSUYA, H ZEMLICKA, J TI UNSATURATED ACYCLIC ANALOGS OF 2'-DEOXYADENOSINE AND THYMIDINE CONTAINING FLUORINE - SYNTHESIS AND BIOLOGICAL-ACTIVITY SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID PROTECTED ALPHA-FLUOROGLYCINES; NUCLEOSIDE ANALOGS; ANTIVIRAL ACTIVITY; HYDROGENATION; REPLICATION; ACIDS AB The syntheses and biological activities of fluorobutynol 11 and (E)- and (Z)-fluorobutenols 8a,d and 9a,d are described. Alkylation of adenine with bromofluorobutyne 13a afforded intermediate 14 which was converted to fluorobutynol 11. Aldehyde 16a and (carbothoxyfluoromethyl)triphenylphosphonium bromide furnished (E)- and (Z)-fluorobutenoates 19a and 20a accompanied by regioisomer 21a. A similar reaction of compound 16d afforded Z- and E-esters 19d and 20d. Reduction of the mixture of 19a and 20a with DIBALH gave (E)- and (Z)-fluoroalkenols 8a and 9a. Similarly, the Z-ester 19d gave (Z)-fluoroalkenol 9d. Both 19d and 20d were reduced with NaBH4 to give (Z)- and (E)-fluoroalkenols 9d and 8d. Hydrogenation of 19a and 20a afforded fluoro ester 23. A similar reduction of 8a and 9a led to fluoro alcohol 24 and the defluorinated product 25 which were separated by chromatography on a Bio-Rad AG 1-X2 (OH-) column. (Z)-Fluorobutenol 9a is a substrate for adenosine deaminase, whereas the E-isomer 8a is inert toward the enzyme. By contrast, analogue 8a inhibited the replication and cytopathic effect of HN-1 in ATH8 cells with an IC50 Of approximately 100 mu M, but the Z-isomer 9a was inactive. This effect was accompanied by 36% cytotoxicity at 100 mu M. Compounds 11 and 8d inhibited the growth of murine leukemia L1210 culture with IC50 = 89 and 60 mu M, respectively. C1 MICHIGAN CANC FDN,DEPT CHEM,DETROIT,MI 48201. WAYNE STATE UNIV,SCH MED,DEPT INTERNAL MED,DETROIT,MI 48201. WAYNE STATE UNIV,SCH MED,DEPT BIOCHEM,DETROIT,MI 48201. NCI,MED BRANCH,EXPTL RETROVIROL SECT,BETHESDA,MD 20892. FU NCI NIH HHS [CA32779] NR 36 TC 17 Z9 17 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA PO BOX 57136, WASHINGTON, DC 20037-0136 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD MAR 17 PY 1995 VL 38 IS 6 BP 875 EP 882 DI 10.1021/jm00006a004 PG 8 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA QN521 UT WOS:A1995QN52100004 PM 7699702 ER PT J AU RAGHAVAN, K BUOLAMWINI, JK FESEN, MR POMMIER, Y KOHN, KW WEINSTEIN, JN AF RAGHAVAN, K BUOLAMWINI, JK FESEN, MR POMMIER, Y KOHN, KW WEINSTEIN, JN TI 3-DIMENSIONAL QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP (QSAR) OF HIV INTEGRASE INHIBITORS - A COMPARATIVE MOLECULAR-FIELD ANALYSIS (COMFA) STUDY SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID DNA INTEGRATION; RETROVIRAL DNA; BINDING; VALIDATION; PREDICTION; RECEPTORS AB We present the results from a comparative molecular field analysis (CoMFA) of a set of flavone analogs that inhibit HIV-1 integrase-mediated cleavage (3'-processing step) and integration (strand transfer step) in vitro. The results indicate a strong correlation between the inhibitory activity of these flavones and the steric and electrostatic fields around them. CoMFA quantitative structure-activity relationship models with considerable predictive ability (cross-validated r(2) as high as 0.8) were obtained. C1 NCI, DEV THERAPEUT PROGRAM, MOLEC PHARMACOL LAB, DIV CANC TREATMENT, BETHESDA, MD 20892 USA. NR 30 TC 93 Z9 95 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0022-2623 EI 1520-4804 J9 J MED CHEM JI J. Med. Chem. PD MAR 17 PY 1995 VL 38 IS 6 BP 890 EP 897 DI 10.1021/jm00006a006 PG 8 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA QN521 UT WOS:A1995QN52100006 PM 7699704 ER PT J AU PRASAD, JVN PARA, KS TUMMINO, PJ FERGUSON, D MCQUADE, TJ LUNNEY, EA RAPUNDALO, ST BATLEY, BL HINGORANI, G DOMAGALA, JM GRACHECK, SJ BHAT, TN LIU, BS BALDWIN, ET ERICKSON, JW SAWYER, TK AF PRASAD, JVN PARA, KS TUMMINO, PJ FERGUSON, D MCQUADE, TJ LUNNEY, EA RAPUNDALO, ST BATLEY, BL HINGORANI, G DOMAGALA, JM GRACHECK, SJ BHAT, TN LIU, BS BALDWIN, ET ERICKSON, JW SAWYER, TK TI NONPEPTIDIC POTENT HIV-1 PROTEASE INHIBITORS - (4-HYDROXY-6-PHENYL-2-OXO-2H-PYRAN-3-YL)THIOMETHANES THAT SPAN P-1-P-2' SUBSITES IN A UNIQUE MODE OF ACTIVE-SITE BINDING SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; DIPEPTIDE ISOSTERES; DESIGN; PROTEINASE; AIDS; ACID AB Using molecular modeling and the information derived from the X-ray crystal structure of HIV-1 protease (HIV PR) complexed with the pyran-2-one 1, a series of (4-hydroxy-6-phenyl-2-oxo-2H-pyran-3-yl)thiomethanes was designed and analyzed as novel, nonpeptidic inhibitors of HIV PR. Structure-activity studies led to the discovery of inhibitor 19 having (RS)-1-(cyclopentylthio)-3-methylbutyl functionalization at the C-3 position, which exhibited a K-c of 33 nM. A X-ray crystallographic structure of 19 bound to HIV PR showed that structural water-301 (inhibitor-flap-bridging water) was displaced by the inhibitor. Interestingly, the enol moiety of the pyran-2-one formed a hydrogen bond directly with Asp125 and with Asp25 via a bridging water molecule, thus illustrating a unique mode of active site binding by an HIV PR inhibitor. The pendant cyclopentyl and isobutyl groups of 19 occupied the S-1' and S-2' binding sites, respectively, whereas the 6-phenyl group occupied a region in between the S-1 and S-3 pockets of HIV PR. Selected compounds were tested for antiviral activity on H9 cells infected with HIV-1(IIIb). A correlation between enzymatic activity and antiviral activity was not found in this series. The best antiviral compound in this series, 18, contained (RS)-3-[cyclopentyl(cyclopentylthio )methyl] functionalization at the C-3 position of the pyran-2-one ring and exhibited a CIC50 of 14 mu M and TC50 of 70 mu M. These studies demonstrate that potent enzyme inhibition can be achieved by inhibitors that span only three subsites. C1 WARNER LAMBERT PARKE DAVIS, PARKE DAVIS PHARMACEUT RES DIV, DEPT BIOCHEM, ANN ARBOR, MI 48106 USA. WARNER LAMBERT PARKE DAVIS, PARKE DAVIS PHARMACEUT RES DIV, DEPT INFECT DIS, ANN ARBOR, MI 48106 USA. WARNER LAMBERT PARKE DAVIS, PARKE DAVIS PHARMACEUT RES DIV, DEPT CARDIOVASC THERAPEUT, ANN ARBOR, MI 48106 USA. PRI DYNCORP, NCI, FREDERICK CANC RES & DEV CTR, STRUCT BIOCHEM PROGRAM, FREDERICK, MD 21702 USA. RP PRASAD, JVN (reprint author), WARNER LAMBERT PARKE DAVIS, PARKE DAVIS PHARMACEUT RES DIV, DEPT CHEM, 2800 PLYMOUTH RD, ANN ARBOR, MI 48106 USA. NR 55 TC 37 Z9 38 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0022-2623 EI 1520-4804 J9 J MED CHEM JI J. Med. Chem. PD MAR 17 PY 1995 VL 38 IS 6 BP 898 EP 905 DI 10.1021/jm00006a007 PG 8 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA QN521 UT WOS:A1995QN52100007 PM 7699705 ER PT J AU WANG, CG LANGER, T KAMATH, PG GU, ZQ SKOLNICK, P FRYER, RI AF WANG, CG LANGER, T KAMATH, PG GU, ZQ SKOLNICK, P FRYER, RI TI COMPUTER-AIDED MOLECULAR MODELING, SYNTHESIS, AND BIOLOGICAL EVALUATION OF 8-(BENZYLOXY-2-PHENYLPYRAZOLO[4,3-C]QUINOLINE AS A NOVEL BENZODIAZEPINE RECEPTOR AGONIST LIGAND SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID BENZODIAZEPINE RECEPTOR; BINDING; ANALOGS AB Using computer-aided conformational analysis, based on molecular dynamics simulation, cluster analysis, and Monte Carlo techniques, we have designed and synthesized compounds in which a benzyloxy substituent has been incorporated into a series of pyrazoloquinoline benzodiazepine receptor (BZR) ligands, Earlier studies had shown that the benzyloxy group could act as part of the agonist pharmacophoric determinant in the beta-carboline ring system. Furthermore, the agonist beta-carboline had been correlated with a binding site orientation and volume fit for an agonist 6-phenylimidazobenzodiazepine carboxylate. The present study was undertaken to determine whether the benzyloxy substituent could be used as an agonist pharmacophoric descriptor for the phenylpyrazolo[4,3-c]quinolin-3-one BZR ligands, The results of a determination of GABA shift ratios for the synthetic ligands indicate that 8-(benzyloxy)-2-phenylpyrazolo[4,3-c]quinolin-3-one can be predicted to be an agonist at the BZR. C1 RUTGERS STATE UNIV, DEPT CHEM, NEWARK, NJ 07102 USA. UNIV INNSBRUCK, INST PHARMAZEUT CHEM, A-6020 INNSBRUCK, AUSTRIA. NIDDKD, NEUROSCI LAB, BETHESDA, MD 20892 USA. NR 20 TC 18 Z9 18 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD MAR 17 PY 1995 VL 38 IS 6 BP 950 EP 957 DI 10.1021/jm00006a014 PG 8 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA QN521 UT WOS:A1995QN52100014 PM 7699711 ER PT J AU GORIN, AA ZHURKIN, VB OLSON, WK AF GORIN, AA ZHURKIN, VB OLSON, WK TI B-DNA TWISTING CORRELATES WITH BASE-PAIR MORPHOLOGY SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE DNA CONFORMATION; HETEROGENEITY; TWISTING; BASE SEQUENCE DEPENDENCE ID T-G-G; CRYSTAL-STRUCTURE; MOLECULAR-STRUCTURE; C-G; CONFORMATIONAL FLEXIBILITY; ANISOTROPIC FLEXIBILITY; POLYNUCLEOTIDE CHAINS; ATOMIC RESOLUTION; HELIX STRUCTURE; ADENINE TRACT AB The observed sequence dependence of the mean twist angles in 38 B-DNA crystal structures can be understood in terms of simple geometrical features of the constituent base-pairs. Structures with low twist appear to unwind in response to severe steric clashes of large exocyclic groups (such as NH2-NH2) in the major and minor grooves, while those with high twist are subjected to lesser contacts (H-O and H-H). We offer a simple clash function that depends on base-pair morphology (i.e. the chemical constitution of base-pairs) and satisfactorily accounts for the twist angles of the ten common Watson-Crick dimer steps both in the solid state and in solution. The twist-clash correlation that we find here still holds when extended to modified bases. In addition to Calladine's purine-purine clashes, we add other close contacts between bases in the grooves, and consider the conformational restrictions on the geometry of the sugar-phosphate backbone (namely, we emphasize the tendency of DNA to conserve virtual backbone length). The significance of this finding is threefold: (1) sequence-dependent DNA twisting is directly involved in protein-DNA interactions; (2) strong correlation between Twist and Roll helps to elucidate the bending of the double helix as a function of base sequence; (3) it is possible to anticipate the effects of chemical modifications on twisting and bending. The mutual correlations of other structural parameters with the twist make this angle a primary determinant of DNA conformational heterogeneity. C1 RUTGERS STATE UNIV,DEPT CHEM,NEW BRUNSWICK,NJ 08903. NCI,MATH BIOL LAB,BETHESDA,MD 20892. RI Gorin, Andrey/B-1545-2014 FU NCI NIH HHS [N10-CO74102]; NIGMS NIH HHS [GM34809, GM20861] NR 93 TC 327 Z9 336 U1 1 U2 8 PU ACADEMIC PRESS (LONDON) LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD MAR 17 PY 1995 VL 247 IS 1 BP 34 EP 48 DI 10.1006/jmbi.1994.0120 PG 15 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QM944 UT WOS:A1995QM94400006 PM 7897660 ER PT J AU FOLEY, TD LINNOILA, M AF FOLEY, TD LINNOILA, M TI NANOMOLAR CONCENTRATIONS OF OUABAIN BLOCK ETHANOL-INDUCIBLE NA+, K+-ATPASE ACTIVITY IN BRAIN SO EUROPEAN JOURNAL OF PHARMACOLOGY-ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY SECTION LA English DT Article DE NA+,K+-ATPASE; ETHANOL; OUABAIN; K+ SYNAPTOSOME ID CARDIAC-GLYCOSIDES; PURKINJE-FIBERS; SODIUM; NA,K-ATPASE; INHIBITION; SENSITIVITY; STIMULATION; MEMBRANES; PUMP AB The effect of low concentrations of ethanol on Na+,K+-ATPase activity, defined as ouabain-inhibitable Rb-86(+) (K+) uptake, was investigated in a crude synaptosome preparation which was subject to minimal subcellular fractionation procedures. Moderate (20-30%) but potent (EC(50) = 3.8 mM) stimulation of total ouabain (1 mM)-inhibitable K+ uptake by ethanol was observed following incubation periods of up to 20 min. The activity of the ethanol-induced component of K+ uptake was antagonized by nanomolar concentrations of ouabain. Thus, the moderate stimulation of total ouabain-inhibitable K+ uptake by ethanol was attributable to the activation of a component of K+ uptake which was very sensitive (VS; IC50 = 2.8 x 10(-10) M) to inhibition by ouabain. Slightly higher concentrations of ouabain (10(-9)-10(-6.6) M) stimulated K+ uptake above control (no ethanol or ouabain) in both the absence and presence of ethanol. The selectivity of the VS-ethanol interaction was demonstrated by the lack of any ethanol effect on two other components of ouabain-inhibitable K+ uptake which accounted for inhibition of K+ uptake by concentrations of ouabain above 10(-6.6) M and were defined as sensitive (S; IC50 = 10(-6) M) and insensitive (I; IC50 = 10(-4) M) to ouabain. These results define the ethanol-inducible component of ouabain-inhibitable Na+,K+-ATPase activity and promote the view that changes in Na+,K+-ATPase-dependent ion translocation may contribute to ethanol intoxication in vivo. RP FOLEY, TD (reprint author), NIAAA,DIV INTRAMURAL CLIN & BIOL RES,LCS,12501 WASHINGTON AVE,ROCKVILLE,MD 20852, USA. NR 37 TC 4 Z9 4 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6917 J9 EUR J PHARM-ENVIRON JI Eur. J. Pharmacol.-Environ. Toxicol. Pharmacol. Sect. PD MAR 16 PY 1995 VL 292 IS 3-4 BP 287 EP 292 DI 10.1016/0926-6917(95)90034-9 PG 6 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA QP913 UT WOS:A1995QP91300011 PM 7796868 ER PT J AU LAVECCHIA, C DAVANZO, B NEGRI, E DECARLI, A BENICHOU, J AF LAVECCHIA, C DAVANZO, B NEGRI, E DECARLI, A BENICHOU, J TI ATTRIBUTABLE RISKS FOR STOMACH-CANCER IN NORTHERN ITALY SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID GASTRIC-CANCER; DIET; EPIDEMIOLOGY AB The proportions of gastric cancer cases attributable (or attributable risks, AR) to consumption of traditional foods (i.e., pasta, rice and maize), low intake of beta-carotene and vitamin C, short duration of use of an electric refrigerator, low educational level, and family history of gastric cancer were computed using data from a case-control study conducted in Northern Italy. Between 1985 and lune 1993 a total of 746 incident, histologically confirmed gastric cancer cases and 2,053 controls admitted to the same network of hospitals for acute, nonneoplastic, non-digestive-tract diseases, unrelated to long-term modifications of diet, were interviewed. The ARs were 48% for low intake of beta-carotene, 40% for high consumption of traditional foods, and 16% for low intake of vitamin C. Overall, these 3 dietary factors explained 73% of the gastric cancer cases in the population. Five percent of all cases were attributable to less than 30 years' use of an electric refrigerator, 15% to low educational level, and 5% to family history of gastric cancer. In individuals over age 60, a greater proportion of cases was attributable to traditional foods, low education and late adoption of electric refrigeration (58% vs. 32% aged under 60), suggesting that correlates of lower social class, influenced lifestyle and dietary habits more markedly in earlier than in more recent generations. According to our estimates, over 3 quarters of the gastric cancer cases in this area are explainable in terms of the risk factors considered. Increased consumption of vitamin C and beta-carotene, and reduced consumption of traditional foods, would help to avoid over 10,000 out of 14,000 stomach-cancer deaths in Italy every year. Consequently, stomach cancer, which is still the third leading cause of cancer death in Italy, would represent only about 2% of all cancer deaths. (C) 1995 Wiley-Liss, Inc. C1 UNIV MILAN,IST NAZL TUMORI,IST STAT MED & BIOMETRIA,I-20133 MILAN,ITALY. NCI,EPIDEMIOL METHODS SECT,ROCKVILLE,MD 20892. RP LAVECCHIA, C (reprint author), IST RIC FARMACOL MARIO NEGRI,VIA ERITREA 62,I-20157 MILAN,ITALY. RI Negri, Eva/B-7244-2013; Decarli, Adriano/C-3129-2017; OI Negri, Eva/0000-0001-9712-8526; Decarli, Adriano/0000-0003-1451-8292; La Vecchia, Carlo/0000-0003-1441-897X NR 22 TC 26 Z9 26 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD MAR 16 PY 1995 VL 60 IS 6 BP 748 EP 752 PG 5 WC Oncology SC Oncology GA QN875 UT WOS:A1995QN87500002 PM 7896439 ER PT J AU CRAVEN, RJ XU, LH WEINER, TM FRIDELL, YW DENT, GA SRIVASTAVA, S VARNUM, B LIU, ET CANCE, WG AF CRAVEN, RJ XU, LH WEINER, TM FRIDELL, YW DENT, GA SRIVASTAVA, S VARNUM, B LIU, ET CANCE, WG TI RECEPTOR TYROSINE KINASES EXPRESSED IN METASTATIC COLON-CANCER SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID GROWTH-FACTOR RECEPTOR; PP60C-SRC PROTEIN-KINASE; HUMAN-BREAST-CANCER; NIH 3T3 CELLS; CARCINOMA CELLS; OVEREXPRESSION; TRANSFORMATION; TUMORIGENESIS; AMPLIFICATION; ACTIVATION AB Using a PCR-based cloning technique, we have isolated a series of DNA fragments coding for tyrosine kinases that are expressed in a metastatic human colon tumor, and have subsequently analyzed their expression pattern at the protein level in human tumors. We identified both the alpha and the beta forms of the platelet-derived growth factor receptor (PDGFR), axl and 8 other genes, including 3 cytoplasmic tyrosine kinases. To study their expression in human colon cancer, we performed Western blots of matched sets of normal tissues and of carcinomas from the same patient. These revealed that the alpha-PDGFR migrates predominantly as a 200-kDa band in 8/8 normal tissues, and as a 170-kDa band in 17/17 malignant tissues, as well as in colonic polyps, suggesting that expression of an isoform of this receptor may be a marker for the progression of colon cancer. Additional studies showed that the Axl receptor tyrosine kinase was expressed at 10-fold higher levels in a peritoneal metastatic nodule than in other normal and malignant tissues. Immunohistochemistry revealed Axl over-expression specifically in the malignant cells of the tumor. This indicates that over-expression and possibly a differential processing event of tyrosine kinase receptors may be involved in colon cancer, and that they are potential markers for the progression of this disease. (C) 1995 Wiley-Liss, Inc. C1 UNIV N CAROLINA,SCH MED,LINEBERGER COMPREHENS CANC CTR,CHAPEL HILL,NC 27599. UNIV N CAROLINA,SCH MED,CURRICULUM GENET & MOLEC BIOL,CHAPEL HILL,NC 27599. UNIV N CAROLINA,SCH MED,DEPT SURG,CHAPEL HILL,NC 27599. UNIV N CAROLINA,SCH MED,DEPT PATHOL,CHAPEL HILL,NC 27599. UNIV N CAROLINA,SCH MED,DEPT MED,CHAPEL HILL,NC 27599. NCI,BETHESDA,MD 20892. AMGEN CORP,THOUSAND OAKS,CA 91320. RI Liu, Edison/C-4141-2008 FU NCI NIH HHS [K08-CA01625, N-01-CN-25421-02, T32-CA09688] NR 29 TC 106 Z9 114 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD MAR 16 PY 1995 VL 60 IS 6 BP 791 EP 797 DI 10.1002/ijc.2910600611 PG 7 WC Oncology SC Oncology GA QN875 UT WOS:A1995QN87500010 PM 7896447 ER PT J AU MISIK, V MIYOSHI, N RIESZ, P AF MISIK, V MIYOSHI, N RIESZ, P TI EPR SPIN-TRAPPING STUDY OF THE SONOLYSIS OF H2O/D2O MIXTURES - PROBING THE TEMPERATURES OF CAVITATION REGIONS SO JOURNAL OF PHYSICAL CHEMISTRY LA English DT Article ID SONOCHEMISTRY; RADICALS; TRAPS; ULTRASOUND; MOLECULES AB High temperatures and pressures are generated during the violent collapse of acoustic cavitation bubbles produced by ultrasound in liquids. The semiclassical model of the temperature dependence of the kinetic isotope effect for H-. and D-. atom formation was used to estimate the effective temperature of the hot cavitation regions in which H-. and D-. atoms are formed by ultrasound-induced pyrolysis of water molecules. The H-. and D-. atoms were formed in argon-saturated H2O and D2O mixtures (1:1) exposed to 50 kHz ultrasound and were detected by spin trapping with the nitrone spin traps N-tert-butyl-alpha-phenylnitrone (PEN), alpha-(4-pyridyl-1 -oxy)-N-tert-butylnitrone (POBN), alpha-(4-pyridyl-1-methyl)-N-tert-butylnitrone (PYBN), and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). The resulting spin adducts were identified and quantified by EPR spectroscopy. Because of the higher stability of H- and D-adducts, the PEN-type spin traps were found to be more suitable than DMPO for the measurement of H-. and D-. atoms (in our experimental system the half-lifes of PBN/H-. and DMPO/H-. were similar to 600 and similar to 40 s, respectively). An isotope effect on spin adduct stability was also observed: the decay rates of PEN-type H-adducts were similar to 1.2 times higher than those of the corresponding D-adducts, and the decay rate of DMPO/H-. was 2.4 times higher than that of DMPO/D-.. The effective temperatures of O-H bond pyrolysis determined from the semiclassical treatment are in the region of similar to 2000-4000 K using the PEN-type spin traps. This estimate may be compared to the temperatures (similar to 5000 K) determined by Suslick ct al. for the gas phase of the cavitation bubbles in alkanes and (approximate to 1900 K) for the interfacial region of the cavitation bubbles [Suslick, K. S., ct al. J. Am. Chem. Sec. 1986, 108, 5641]. C1 NCI,RADIAT BIOL BRANCH,BETHESDA,MD 20892. NR 33 TC 114 Z9 115 U1 3 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA PO BOX 57136, WASHINGTON, DC 20037-0136 SN 0022-3654 J9 J PHYS CHEM-US JI J. Phys. Chem. PD MAR 16 PY 1995 VL 99 IS 11 BP 3605 EP 3611 DI 10.1021/j100011a030 PG 7 WC Chemistry, Physical SC Chemistry GA QN284 UT WOS:A1995QN28400030 ER PT J AU BORICK, SS DEBENEDETTI, PG SASTRY, S AF BORICK, SS DEBENEDETTI, PG SASTRY, S TI A LATTICE MODEL OF NETWORK-FORMING FLUIDS WITH ORIENTATION-DEPENDENT BONDING - EQUILIBRIUM, STABILITY, AND IMPLICATIONS FOR THE PHASE-BEHAVIOR OF SUPERCOOLED WATER SO JOURNAL OF PHYSICAL CHEMISTRY LA English DT Article ID LONG-TIME REGIME; LIQUID WATER; MOLECULAR-DYNAMICS; HEAVY-WATER; ISOTHERMAL COMPRESSIBILITY; AQUEOUS-SOLUTIONS; LOW-TEMPERATURES; ICE-I; TRANSITIONS; DENSITY AB We use a lattice model with orientation-dependent interactions to study network-forming fluids, such as water and silica. Bonds can form between pairs of molecules that have correct mutual orientation and correct separation; they can be weakened by the presence of other molecules sufficiently close to a bonded pair. These interactions give rise to competition between bonded states of low energy, density, and entropy and nonbonded states of high energy, density, and entropy. By suitable choice of parameters, the mean-field solution of the model yields the two different scenarios (stability limit conjecture and second critical point) that have been proposed to explain the anomalous behavior of supercooled water. The model's generality suggests that similar behavior can occur in other network-forming fluids. C1 PRINCETON UNIV,DEPT CHEM ENGN,PRINCETON,NJ 08544. NIH,DIV COMP RES & TECHNOL,PHYS SCI LAB,BETHESDA,MD 20892. NR 85 TC 95 Z9 95 U1 2 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA PO BOX 57136, WASHINGTON, DC 20037-0136 SN 0022-3654 J9 J PHYS CHEM-US JI J. Phys. Chem. PD MAR 16 PY 1995 VL 99 IS 11 BP 3781 EP 3792 DI 10.1021/j100011a054 PG 12 WC Chemistry, Physical SC Chemistry GA QN284 UT WOS:A1995QN28400054 ER PT J AU BOZZETTE, SA FINKELSTEIN, DM SPECTOR, SA FRAME, P POWDERLY, WG HE, WL PHILLIPS, L CRAVEN, D VANDERHORST, C FEINBERG, J AF BOZZETTE, SA FINKELSTEIN, DM SPECTOR, SA FRAME, P POWDERLY, WG HE, WL PHILLIPS, L CRAVEN, D VANDERHORST, C FEINBERG, J TI A RANDOMIZED TRIAL OF 3 ANTIPNEUMOCYSTIS AGENTS IN PATIENTS WITH ADVANCED HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID PNEUMOCYSTIS-CARINII PNEUMONIA; AEROSOLIZED PENTAMIDINE; TRIMETHOPRIM-SULFAMETHOXAZOLE; PRIMARY PROPHYLAXIS; SECONDARY PROPHYLAXIS; DAPSONE-PYRIMETHAMINE; PRIMARY PREVENTION; AIDS; COTRIMOXAZOLE; ZIDOVUDINE AB Background. We evaluated the effectiveness of three treatment strategies for the prevention of a first episode of Pneumocystis carinii pneumonia in patients infected with the human immunodeficiency virus (HIV). Methods. In an open-label trial, 843 patients with HIV infection and fewer than 200 CD4+ cells per cubic millimeter received zidovudine plus one of three randomly assigned prophylactic agents, beginning with trimethoprim-sulfamethoxazole, dapsone, or aerosolized pentamidine and followed by a defined sequence of other drugs to be used in cases of intolerance. Results. The estimated 36-month cumulative risks of P. carinii pneumonia were 18 percent, 17 percent, and 21 percent in the trimethoprim-sulfamethoxazole, dapsone, and aerosolized-pentamidine groups, respectively (P = 0.22). The difference in risk among treatment strategies was negligible in patients entering the study with 100 or more CD4+ lymphocytes per cubic millimeter. In those entering with fewer than 100 CD4+ cells per cubic millimeter, the risk was 33 percent with aerosolized pentamidine, as compared with 19 percent with trimethoprim-sulfamethoxazole and 22 percent with dapsone (P=0.04). The lowest failure rates occurred in patients receiving trimethoprim-sulfamethoxazole, acid failures were more common with 50 mg of dapsone than with 100 mg. Toxoplasmosis developed in less than 3 percent of patients. Of the patients assigned to the two systemic therapies, only 23 percent were receiving their assigned drug and dose when they completed the study. The median survival was approximately 39 months in all three groups, and the mortality attributable to P. carinii pneumonia was only 1 percent. Conclusions. In patients with advanced HIV infection, the three treatment strategies we examined have similar effectiveness in preventing P. carinii pneumonia. Strategies that start with trimethoprim-sulfamethoxazole or with high-dose dapsone, rather than aerosolized pentamidine, are superior in patients with fewer than 100 CD4+ lymphocytes per cubic millimeter. C1 UNIV CALIF SAN DIEGO,LA JOLLA,CA 92093. RAND CORP,SANTA MONICA,CA. HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. UNIV CINCINNATI,CINCINNATI,OH. WASHINGTON UNIV,SCH MED,ST LOUIS,MO. FRONTIER SCI TECHNOL & RES FDN,BUFFALO,NY. BOSTON UNIV,BOSTON,MA 02215. UNIV N CAROLINA,CHAPEL HILL,NC. NIAID,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,BALTIMORE,MD. RP BOZZETTE, SA (reprint author), VET AFFAIRS MED CTR,MAIL CODE 111N-1,3350 LA JOLLA VILLAGE DR,SAN DIEGO,CA 92161, USA. NR 24 TC 226 Z9 230 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 16 PY 1995 VL 332 IS 11 BP 693 EP 699 DI 10.1056/NEJM199503163321101 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA QL788 UT WOS:A1995QL78800001 PM 7854375 ER PT J AU POWDERLY, WG FINKELSTEIN, DM FEINBERG, J FRAME, P HE, WL VANDERHORST, C KOLETAR, SL EYSTER, ME CAREY, J WASKIN, H HOOTON, TM HYSLOP, N SPECTOR, SA BOZZETTE, SA AF POWDERLY, WG FINKELSTEIN, DM FEINBERG, J FRAME, P HE, WL VANDERHORST, C KOLETAR, SL EYSTER, ME CAREY, J WASKIN, H HOOTON, TM HYSLOP, N SPECTOR, SA BOZZETTE, SA TI A RANDOMIZED TRIAL COMPARING FLUCONAZOLE WITH CLOTRIMAZOLE TROCHES FOR THE PREVENTION OF FUNGAL-INFECTIONS IN PATIENTS WITH ADVANCED HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID PNEUMOCYSTIS-CARINII PNEUMONIA; AEROSOLIZED PENTAMIDINE; CRYPTOCOCCAL MENINGITIS; TRIMETHOPRIM SULFAMETHOXAZOLE; AMPHOTERICIN-B; PROPHYLAXIS; AIDS; COMPLEX; THERAPY; RELAPSE AB Background. Cryptococcal meningitis and other serious fungal infections are common complications in patients infected with the human immunodeficiency virus (HIV). Fluconazole is effective for long-term suppression of many fungal infections, but its effectiveness as primary prophylaxis had not been adequately evaluated. Methods. We conducted a prospective, randomized trial that compared fluconazole (200 mg per day) with clotrimazole troches (10 mg taken five times daily) in patients who were also participating in a randomized trial of primary prophylaxis for Pneumocystis carinii pneumonia. Results. After a median follow-up of 35 months, invasive fungal infections had developed in 4.1 percent of the patients in the fluconazole group (9 of 217) and in 10.9 percent of those in the clotrimazole group (23 of 211, relative hazard, as adjusted for the CD4+ count, 3.3; 95 percent confidence interval, 1.5 to 7.6). Of the 32 invasive fungal infections, 17 were cryptococcosis (2 in the fluconatole group and 15 in the clotrimazole group; adjusted relative hazard, 8.5; 95 percent confidence interval, 1.9 to 37.6). The benefit of fluconazole was greater for the patients with 50 or fewer CD4+ cells per cubic millimeter than for the patients with higher counts. Fluconazole was also effective in preventing esophageal candidiasis (adjusted relative hazard, 5.8; 95 percent confidence interval, 1.7 to 20.0; P=0.004) and confirmed and presumed oropharyngeal candidiasis (5.7 and 38.1 cases per 100 person-years of follow-up in the fluconazole and clotrimazole groups, respectively; P < 0.001). Survival was similar in the two groups. Conclusions. Fluconazole taken prophylactically reduces the frequency of cryptococcosis, esophageal candidiasis, and superficial fungal infections in HIV-infected patients, especially those with 50 or fewer CD4+ lymphocytes per cubic millimeter, but the drug does not reduce overall mortality. C1 VET AFFAIRS MED CTR,SAN DIEGO,CA 92161. WASHINGTON UNIV,SCH MED,ST LOUIS,MO. HARVARD UNIV,SCH PUBL HLTH,CTR STAT & DATA ANAL,BOSTON,MA 02115. NIAID,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,BALTIMORE,MD. UNIV CINCINNATI,CINCINNATI,OH. UNIV N CAROLINA,CHAPEL HILL,NC. OHIO STATE UNIV,COLUMBUS,OH 43210. PENN STATE UNIV,COLL MED,HERSHEY,PA. CASE WESTERN RESERVE UNIV,SCH MED,CLEVELAND,OH. DUKE UNIV,DURHAM,NC. UNIV WASHINGTON,SEATTLE,WA 98195. TULANE UNIV,NEW ORLEANS,LA 70118. UNIV CALIF SAN DIEGO,LA JOLLA,CA 92093. RAND CORP,SANTA MONICA,CA. NR 18 TC 241 Z9 247 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 16 PY 1995 VL 332 IS 11 BP 700 EP 705 DI 10.1056/NEJM199503163321102 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA QL788 UT WOS:A1995QL78800002 PM 7854376 ER PT J AU MOFENSON, LM NUGENT, R AF MOFENSON, LM NUGENT, R TI PROPHYLACTIC IMMUNE GLOBULIN IN CHILDREN WITH HIV DISEASE SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter RP MOFENSON, LM (reprint author), NIH,BETHESDA,MD 20892, USA. NR 8 TC 1 Z9 1 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 16 PY 1995 VL 332 IS 11 BP 750 EP 751 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA QL788 UT WOS:A1995QL78800020 PM 7854391 ER PT J AU PEEBLES, RS MALISZEWSKI, CR SATO, TA HANLEYHYDE, J MAROULAKOU, IG HUNZIKER, R SCHNECK, JP GREEN, JE AF PEEBLES, RS MALISZEWSKI, CR SATO, TA HANLEYHYDE, J MAROULAKOU, IG HUNZIKER, R SCHNECK, JP GREEN, JE TI ABNORMAL B-CELL FUNCTION IN HTLV-I-TAX TRANSGENIC MICE SO ONCOGENE LA English DT Article DE HTLV-I; GROWTH FACTORS; B-CELLS; TRANSGENIC MICE; LYMPHOPROLIFERATION; AUTOIMMUNE DISEASE ID VIRUS TYPE-I; TROPICAL SPASTIC PARAPARESIS; COLONY-STIMULATING FACTOR; PRIMARY SJOGRENS-SYNDROME; EPSTEIN-BARR-VIRUS; HEPATITIS-C VIRUS; INTERLEUKIN-2 RECEPTOR; MOUSE MODEL; ACTIVATION; GENE AB Transgenic mice that carry the HTLV-I Tax gene develop an exocrinopathy with some similarities to Sjoegren's syndrome. Our experiments reveal that these mice have lymphadenopathy and splenomegaly composed primarily of B lymphocytes, as well as abnormal levels of secreted immunoglobulins. To gain insight into whether the lymphadenopathy manifested by these transgenic mice was the result of induction of cytokines by Tax, we utilized cell lines from these mice to study in vitro B-cell responses. Conditioned media (CM) derived from the cell lines caused B-cells to proliferate when a second signal, surface Ig cross-linking, was provided. The CM also caused a marked enhancement of IgM secretion by spleen cells or by purified B-cells treated with supplemental cytokines. The B-cell proliferative response and enhanced IgM secretion have not been attributed to a known cytokine. These results suggest that the CM from the cell lines contain a factor(s) involved in novel pathways of B-cell growth and differentiation that may participate in the pathologic development of autoimmune disease. C1 NCI,MOLEC ONCOL LAB,FREDERICK,MD 21702. JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV CLIN IMMUNOL,BALTIMORE,MD 21224. NCI,GENET LAB,BETHESDA,MD 20892. IMMUNEX CORP,DEPT CELLULAR IMMUNOL,BETHESDA,MD 20892. NR 40 TC 17 Z9 17 U1 1 U2 1 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HANTS, ENGLAND RG21 2XS SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAR 16 PY 1995 VL 10 IS 6 BP 1045 EP 1051 PG 7 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA QN353 UT WOS:A1995QN35300003 PM 7700628 ER PT J AU SMITH, ML CHEN, IT ZHAN, QM OCONNOR, PM FORNACE, AJ AF SMITH, ML CHEN, IT ZHAN, QM OCONNOR, PM FORNACE, AJ TI INVOLVEMENT OF THE P53 TUMOR-SUPPRESSOR IN REPAIR OF UV-TYPE DNA-DAMAGE SO ONCOGENE LA English DT Article DE P53; UV; DNA REPAIR; GADD45; HPV E6; CHECKPOINTS ID EXCISION REPAIR; IONIZING-RADIATION; CELLULAR-RESPONSE; ESCHERICHIA-COLI; PROTEIN; GENE; ACTIVATION; CHECKPOINT; APOPTOSIS; AGENTS AB The tumor suppressor p53 plays a central role in the cellular responses to genotoxic stress. Besides its well known role in activation of the G(1) checkpoint after exposure to agents like ionizing radiation and its role in apoptosis, the possibility exists that p53 may have additional roles, such as in DNA repair. For example, p53, is known to bind to single strand DNA such as would occur during repair events, and the proteins encoded by two p53-regulated genes have previously been found to bind to at least one protein involved in DNA damage processing including nucleotide excision repair (NER). NER is an important and versatile DNA repair mechanism, which is the major pathway for repair of u.v.-type lesions and damage by a variety of important carcinogens and mutagens. If components of the p53 pathway are involved in NER, then disruption of p53 function by mutations or expression of certain viral proteins could have important implications in carcinogenesis and cancer treatment. In the present study we show that disruption of normal p53 function in human colon carcinoma RKO cells with either the human papillomavirus E6 oncoprotein or a dominant-negative mutant p53 transgene results in reduced repair of u.v.-induced DNA damage. The E6 and mutant p53-containing cell lines demonstrated reduced repair of u.v.-induced DNA lesions in host cell reactivation experiments with reporter plasmids, and reduced repair in in vitro DNA repair assays. With this in vitro assay, extracts from the E6- and mutant p53-containing lines also showed loss of induced repair following cellular u.v.-irradiation. The reduced DNA repair activity of the transfected cell lines also correlated with reduced clonogenic survival following u.v.-irradiation. These results indicate that p53 and/or p53-regulated gene products function in the NER pathway and that this process is inducible by DNA damage. RP SMITH, ML (reprint author), NCI,DIV CANC TREATMENT,MOLEC PHARMACOL LAB,DEV THERAPEUT PROGRAM,BLDG 37,ROOM 5C09,BETHESDA,MD 20892, USA. RI Fornace, Albert/A-7407-2008 OI Fornace, Albert/0000-0001-9695-085X NR 64 TC 355 Z9 357 U1 2 U2 11 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HANTS, ENGLAND RG21 2XS SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAR 16 PY 1995 VL 10 IS 6 BP 1053 EP 1059 PG 7 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA QN353 UT WOS:A1995QN35300004 PM 7700629 ER PT J AU JONES, TL KARAVANOVA, I MAENO, M ONG, RC KUNG, HF DAAR, IO AF JONES, TL KARAVANOVA, I MAENO, M ONG, RC KUNG, HF DAAR, IO TI EXPRESSION OF AN AMPHIBIAN HOMOLOG OF THE EPH FAMILY OF RECEPTOR TYROSINE KINASES IS DEVELOPMENTALLY-REGULATED SO ONCOGENE LA English DT Article DE EPH; TYROSINE KINASE; XENOPUS LAEVIS ID XENOPUS-EMBRYOS; PROTEIN-KINASES; CDNA CLONING; W-LOCUS; GENE; MOUSE; DROSOPHILA; IDENTIFICATION; ENCODES; CHICKEN AB In order to study the function of tyrosine kinase receptors during Xenopus development, we have isolated Xek (Xenopus Elk-like kinase), a tyrosine kinase receptor, which shows significant homology to rat Elk and chicken cek5, members of the Eph family. Xek exists as a maternally expressed mRNA which decreases in expression at the mid blastula transition and reappears at late neurulation in Xenopus, Xek mRNA is expressed at higher levels in the anterior and dorsal regions of embryonic stages 16, 24 and 37. In adult Xenopus tissues, Xek appears to be ubiquitously expressed with higher expression observed in brain and ovary, In situ hybridization analysis demonstrates localized mRNA expression in the brain, brachial arches, trigeminal facial ganglion, and the retina of the swimming tadpole stage of development. The similarities in sequence and expression pattern suggest that Xek is an amphibian member of the Eph family and may play a role in the development or function of the central nervous system. C1 NCI,FREDERICK CANC RES & DEV CTR,LEUKOCYTE BIOL LAB,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,BIOCHEM PHYSIOL LAB,FREDERICK,MD 21702. OI Daar, Ira/0000-0003-2657-526X NR 48 TC 28 Z9 31 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HANTS, ENGLAND RG21 2XS SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAR 16 PY 1995 VL 10 IS 6 BP 1111 EP 1117 PG 7 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA QN353 UT WOS:A1995QN35300011 PM 7700636 ER PT J AU ZIMONJIC, DB ALIMANDI, M MIKI, T POPESCU, NC KRAUS, MH AF ZIMONJIC, DB ALIMANDI, M MIKI, T POPESCU, NC KRAUS, MH TI LOCALIZATION OF THE HUMAN HER4/ERBB-4 GENE TO CHROMOSOME-2 SO ONCOGENE LA English DT Note DE RECEPTOR TYROSINE KINASE; ERBB FAMILY; GENE MAPPING; FISH ID FACTOR RECEPTOR FAMILY; FRAGILE SITES; TRANSLOCATION (2-13)(Q37-Q14); ALVEOLAR RHABDOMYOSARCOMA; NERVOUS-SYSTEM; ONCOGENE; MEMBER; HER4/P180(ERBB4); EXPRESSION; HEREGULIN AB The HER4/erbB-4 gene has been isolated as the fourth member of the human EGFR subfamily of tyrosine kinases and has been reported to encode a receptor for NDF/heregulin. In the present study we determined the chromosomal location of the HER4/erbB-4 gene within the human genome. Using human cDNA probes in fluorescence in situ hybridization (FISH), we mapped the HER4/erbB-4 gene to human chromosome 2q33.3-34. This finding established that also the HER4/erbB-4 gene is located in close vicinity of homeobox and collagen gene loci, as is the case for the related EGFR, erbB-2/neu and erbB-3. Aberrations of this chromosomal region associated with T cell leukemias and lymphomas as well as alveolar rhabdomyosarcomas raise the possibility that HER4/erbB-4 might be activated in these tumour types. C1 NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892. NCI,BIOL LAB,BETHESDA,MD 20892. NR 31 TC 26 Z9 30 U1 1 U2 3 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HANTS, ENGLAND RG21 2XS SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAR 16 PY 1995 VL 10 IS 6 BP 1235 EP 1237 PG 3 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA QN353 UT WOS:A1995QN35300026 PM 7700649 ER PT J AU BATES, MN SMITH, AH CANTOR, KP AF BATES, MN SMITH, AH CANTOR, KP TI CASE-CONTROL STUDY OF BLADDER-CANCER AND ARSENIC IN DRINKING-WATER SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE ARSENIC; BLADDER NEOPLASMS; SMOKING; WATER POLLUTANTS; WATER SUPPLY ID DISEASE ENDEMIC AREA; MALIGNANT NEOPLASMS; RESPIRATORY CANCER; SODIUM ARSENITE; COPPER SMELTER; LUNG-CANCER; WELL WATER; MORTALITY; EXPOSURE; CARCINOGENESIS AB Mortality from several cancers, including bladder cancer, is elevated in a Taiwanese population exposed to high levels of arsenic in drinking water. Data from the Utah respondents to the National Bladder Cancer Study conducted in 1978 were used to evaluate these associations in a US population exposed to measurable, but much lower, levels of drinking water arsenic. Two indices of cumulative arsenic exposure were used, one representing total cumulative exposure (index 1) and the other, intake concentration (index 2). Overall, there was no association of bladder cancer with either measure; however, among smokers, but not among nonsmokers, positive trends in risk were found for exposures estimated for decade-long time periods, especially in the 30- to 39-year period prior to diagnosis. Exposures were in the range 0.5-160 mu g/liter (mean, 5.0 mu g/liter). The data raise the possibility that smoking potentiates the effect of arsenic on risk of bladder cancer. However, the risk estimates obtained are much higher than predicted on the basis of the results of the Taiwanese studies, raising concerns about bias or the role of chance. Confirmatory studies are needed. C1 UNIV CALIF BERKELEY,SCH PUBL HLTH,DEPT BIOMED & ENVIRONM HLTH SCI,BERKELEY,CA 94720. NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. RI Smith, Allan/F-9249-2011 FU NIEHS NIH HHS [ES-01896, P42 ES-04705] NR 36 TC 168 Z9 169 U1 0 U2 2 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD MAR 15 PY 1995 VL 141 IS 6 BP 523 EP 530 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA QN480 UT WOS:A1995QN48000006 PM 7900719 ER PT J AU ROWLAND, AS BAIRD, DD SHORE, DL WEINBERG, CR SAVITZ, DA WILCOX, AJ AF ROWLAND, AS BAIRD, DD SHORE, DL WEINBERG, CR SAVITZ, DA WILCOX, AJ TI NITROUS-OXIDE AND SPONTANEOUS-ABORTION IN FEMALE DENTAL ASSISTANTS SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE ABORTION, SPONTANEOUS; AIR POLLUTANTS, OCCUPATIONAL; COHORT STUDIES; DENTAL STAFF; NITROUS OXIDE; OCCUPATIONAL DISEASES ID SPRAGUE-DAWLEY RATS; ANESTHETIC-GASES; OCCUPATIONAL EXPOSURE; FOLINIC ACID; NITRIC-OXIDE; WOMEN; MALFORMATIONS; HOMOCYSTEINE; EMPLOYMENT; PREGNANCY AB The relation between anesthetic gas exposure and spontaneous abortion remains unresolved. We examined the effect of nitrous oxide on spontaneous abortion among female dental assistants. Questionnaires were sent to 7,000 dental assistants aged 18-39 years who were registered in California in 1987; 4,856 (69%) responded. Analysis was based on 1,465 respondents whose most recent pregnancy was conceived while working full time. Women were asked how many times a week they worked with nitrous oxide during this pregnancy and whether the excess gas was scavenged (vented). Relative risk of spontaneous abortion (through week 20) was calculated using a person-week model. This allowed women with current pregnancies (13%) or induced abortions (10%) to be included for appropriate time periods of risk. A total of of 101 pregnancies (7%) ended as spontaneous abortions. An elevation of risk of spontaneous abortions was seen among women who worked with nitrous oxide for 3 or more per week in offices not using scavenging equipment (relative risk = 2.6, 95% confidence interval 1.3-5.0, adjusted for age, smoking and number of amalgams prepared per week), but not among those using nitrous oxide in offices with scavenging equipment. This relation changed little when analyses were restricted to confirmed pregnancies or examined for several types of potential bias. Scavenging equipment appears to be important in protecting the reproductive health of women working with nitrous oxide. C1 WESTAT CORP,DURHAM,NC. NIEHS,STAT & BIOMATH BRANCH,RES TRIANGLE PK,NC 27709. UNIV N CAROLINA,SCH PUBL HLTH,DEPT EPIDEMIOL,CHAPEL HILL,NC. RP ROWLAND, AS (reprint author), NIEHS,EPIDEMIOL BRANCH A3 05,POB 12233,RES TRIANGLE PK,NC 27709, USA. OI Wilcox, Allen/0000-0002-3376-1311; Baird, Donna/0000-0002-5544-2653 NR 53 TC 115 Z9 116 U1 0 U2 5 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD MAR 15 PY 1995 VL 141 IS 6 BP 531 EP 538 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA QN480 UT WOS:A1995QN48000007 PM 7900720 ER PT J AU DAQUILA, RT JOHNSON, VA WELLES, SL JAPOUR, AJ KURITZKES, DR DEGRUTTOLA, V REICHELDERFER, PS COOMBS, RW CRUMPACKER, CS KAHN, JO RICHMAN, DD AF DAQUILA, RT JOHNSON, VA WELLES, SL JAPOUR, AJ KURITZKES, DR DEGRUTTOLA, V REICHELDERFER, PS COOMBS, RW CRUMPACKER, CS KAHN, JO RICHMAN, DD TI ZIDOVUDINE RESISTANCE AND HIV-1 DISEASE PROGRESSION DURING ANTIRETROVIRAL THERAPY SO ANNALS OF INTERNAL MEDICINE LA English DT Article DE ZIDOVUDINE; DRUG RESISTANCE; HUMAN IMMUNODEFICIENCY VIRUS-1; ANTIVIRAL AGENTS; DIDANOSINE ID HUMAN-IMMUNODEFICIENCY-VIRUS; SYNCYTIUM-INDUCING PHENOTYPE; REVERSE-TRANSCRIPTASE; SENSITIVITY; CAPACITY; CHILDREN; MUTATION; INVITRO; AIDS AB Objective: To evaluate the association between resistance of human immunodeficiency virus type 1 (HIV-1) to zidovudine and clinical progression. Design: Retrospective analysis of specimens from patients in the AIDS Clinical Trials Group (ACTG) protocol 116B/117, a randomized comparison of didanosine with continued zidovudine therapy in patients with advanced HIV-1 disease who had received 16 weeks or more of previous zidovudine therapy. Setting: Participating ACTG virology laboratories. Patients: 187 patients with baseline HIV-1 isolates. Measurements: Zidovudine susceptibility testing and assays for syncytium-inducing phenotype were done on baseline HIV-1 isolates. Relative hazards for clinical progression or death associated with baseline clinical, virologic, and immunologic factors were determined from Cox proportional hazards regression models. Results: Compared with other patients, 15% (26 of 170) with isolates showing high-level zidovudine resistance (50% inhibitory zidovudine concentration greater than or equal to 1.0 mu M) had 1.74 times the risk for progressing to a new AIDS-defining event or death (95% CI, 1.00 to 3.03) and 2.78 times the risk for death (CI, 1.21 to 6.39) in analyses that controlled for baseline CD4(+) T-lymphocyte count, syncytium-inducing HIV-1 phenotype, disease stage, and randomized treatment assignment. The clinical benefit of didanosine was not limited to patients with highly zidovudine-resistant baseline HIV-1 isolates. Conclusions: High-level resistance of HIV-1 to zidovudine predicted more rapid clinical progression and death when adjusted for other factors. However, patients with advanced HIV-1 disease may benefit from a change in monotherapy from zidovudine to didanosine whether high-level HIV-1 resistance to zidovudine is present or absent, and laboratory assessment of zidovudine resistance is not necessary for deciding when to switch monotherapy from zidovudine to didanosine. C1 HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. UNIV ALABAMA,SCH MED,BIRMINGHAM,AL. VET AFFAIRS MED CTR,BIRMINGHAM,AL. UNIV COLORADO,HLTH SCI CTR,DENVER,CO. VET AFFAIRS MED CTR,DENVER,CO. NIAID,BETHESDA,MD 20892. UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. SAN FRANCISCO GEN HOSP,AIDS PROGRAM,SAN FRANCISCO,CA. UNIV CALIF SAN DIEGO,SAN DIEGO,CA 92103. VET AFFAIRS MED CTR,LA JOLLA,CA. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,BOSTON,MA. HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,BOSTON,MA. FU NIAID NIH HHS [AI 27659, AI 32775, AI 29193] NR 38 TC 231 Z9 232 U1 0 U2 1 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD MAR 15 PY 1995 VL 122 IS 6 BP 401 EP 408 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA QM266 UT WOS:A1995QM26600001 PM 7856987 ER PT J AU OHASHI, T MASUDA, M RUSCETTI, SK AF OHASHI, T MASUDA, M RUSCETTI, SK TI INDUCTION OF SEQUENCE-SPECIFIC DNA-BINDING FACTORS BY ERYTHROPOIETIN AND THE SPLEEN FOCUS-FORMING VIRUS SO BLOOD LA English DT Note ID SIGNAL-TRANSDUCTION PATHWAY; TYROSINE PHOSPHORYLATION; CYTOKINE RECEPTORS; INTERFERON-ALPHA; ENVELOPE GENE; GROWTH-FACTOR; PROTEIN; ERYTHROLEUKEMIA; TRANSCRIPTION; ACTIVATION AB The signal transduction mechanism of erythropoietin (Epo), which regulates growth and differentiation of erythroid cells, is still unclear. Recent studies showing the activation by various ligands of a group of proteins called Stat (signal transducers and activators of transcription) proteins raised the possibility that such proteins may also be involved in the Epo signal transduction pathway. In this report, we show that Epo induces factors that specifically bind to the sis-inducible element and the gamma response region of the Fc gamma receptor factor I gene in the Epo-dependent mouse erythroleukemia cell line HCD-57. These factors contain phosphotyrosine and antibodies against Stat1 and Stat3 proteins reacted with them. In HCD-57 cells infected with Friend spleen focus-forming virus, which now grow in an Epo-independent manner, the DNA-binding factors were constitutively activated even in the absence of Epo. These results suggest that the factors induced by Epo contain components identical or related to known Stat proteins. It is also suggested that continuous activation of these DNA-binding factors may be responsible for the ability of spleen focus-forming virus to abrogate the Epo-dependence of HCD-57 cells and cause erythroleukemia in susceptible mice. (C) 1995 by The American Society of Hematology. C1 NCI,FREDERICK CANC RES & DEV CTR,MOLEC ONCOL LAB,FREDERICK,MD 21702. RI Ohashi, Takashi/C-4671-2012 OI Ohashi, Takashi/0000-0002-3769-4224 NR 47 TC 63 Z9 64 U1 0 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD MAR 15 PY 1995 VL 85 IS 6 BP 1454 EP 1462 PG 9 WC Hematology SC Hematology GA QN289 UT WOS:A1995QN28900006 PM 7888668 ER PT J AU KNIPPING, E DEBATIN, KM STRICKER, K HEILIG, B EDER, A KRAMMER, PH AF KNIPPING, E DEBATIN, KM STRICKER, K HEILIG, B EDER, A KRAMMER, PH TI IDENTIFICATION OF SOLUBLE APO-1 IN SUPERNATANTS OF HUMAN B-CELL AND T-CELL LINES AND INCREASED SERUM LEVELS IN B-CELL AND T-CELL LEUKEMIAS SO BLOOD LA English DT Article ID TUMOR-NECROSIS-FACTOR; IMMUNOLOGICAL CROSS-REACTIVITY; GROWTH-FACTOR RECEPTOR; FACTOR-ALPHA; HUMAN-URINE; LYMPHOCYTIC-LEUKEMIA; MONOCLONAL-ANTIBODY; MOLECULAR-CLONING; APOPTOSIS GENE; TNF RECEPTOR AB The cell-surface protein APO-1 is a member of the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. APO-1 mediates apoptosis in susceptible cells upon stimulation with the monoclonal antibody anti-APO-1 or upon binding of its natural ligand. Soluble receptors had previously been identified for most members of the NGF/TNF receptor superfamily. Recently, a soluble form of APO-1 (sAPO-1) was described. We established a sandwich enzyme-linked immunosorbent assay to detect sAPO-1 in culture supernatants of human cell lines and in human sera. sAPO-1 was found in culture supernatants of different human B- and T-cell lines. Molecular weights of sAPO-1 and membrane APO-1 were similar. In addition, in comparison to healthy donors, sera from patients with different high- and low-grade malignant B- and T-cell leukemias and lymphomas contained increased levels of sAPO-1. These findings may have implications for the growth of leukemias and the diagnostic monitoring of individual patients. (C) 1995 by The American Society of Hematology. C1 GERMAN CANC RES CTR,DIV IMMUNOGENET,TUMORIMMUNOL PROGRAM,D-69120 HEIDELBERG,GERMANY. UNIV HEIDELBERG,CHILDRENS HOSP,HEMATOL ONCOL SECT,W-6900 HEIDELBERG,GERMANY. UNIV HEIDELBERG,MED KLIN 5,HEIDELBERG,GERMANY. NIH,BIOL MODIFIERS PROGRAM,BETHESDA,MD 20892. BENDER MEDSYST,VIENNA,AUSTRIA. RI Debatin, Klaus-Michael/J-9704-2014 OI Debatin, Klaus-Michael/0000-0002-8397-1886 NR 64 TC 137 Z9 143 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD MAR 15 PY 1995 VL 85 IS 6 BP 1562 EP 1569 PG 8 WC Hematology SC Hematology GA QN289 UT WOS:A1995QN28900020 PM 7534137 ER PT J AU KINGMA, DW RAFFELD, M JAFFE, ES AF KINGMA, DW RAFFELD, M JAFFE, ES TI DIFFERENTIAL-DIAGNOSIS OF CD3(+), CD56(+) T-CELL LEUKEMIAS SO BLOOD LA English DT Letter ID GAMMA-DELTA; LOCALIZATION; EXPRESSION; SUBSET; BETA RP KINGMA, DW (reprint author), NCI,PATHOL LAB,BLDG 10,BETHESDA,MD 20892, USA. NR 8 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD MAR 15 PY 1995 VL 85 IS 6 BP 1675 EP 1676 PG 2 WC Hematology SC Hematology GA QN289 UT WOS:A1995QN28900037 PM 7534142 ER PT J AU ZARATEOSORNO, A ROMAN, LN KINGMA, DW MENESESGARCIA, A JAFFE, ES AF ZARATEOSORNO, A ROMAN, LN KINGMA, DW MENESESGARCIA, A JAFFE, ES TI HODGKINS-DISEASE IN MEXICO - PREVALENCE OF EPSTEIN-BARR-VIRUS SEQUENCES AND CORRELATIONS WITH HISTOLOGIC SUBTYPE SO CANCER LA English DT Article DE HODGKINS DISEASE; LYMPHOMAS; EPSTEIN-BARR VIRUS; EPIDEMIOLOGY; IN SITU HYBRIDIZATION; IMMUNOPHENOTYPE ID REED-STERNBERG CELLS; POLYMERASE CHAIN-REACTION; INSITU HYBRIDIZATION; BIOTINYLATED PROBES; LYMPH-NODE; B-CELL; EXPRESSION; GENOMES; EBV; ASSOCIATION AB Background. The Epstein-Barr virus (EBV) has been linked to several human malignancies, including Hodgkin's disease (HD). In addition, epidemiologic studies have shown differences in HD occurrence in different parts of the world. The authors studied 27 cases of Hodgkin's disease from Mexico to determine the prevalence of EBV in HD in this developing nation. Methods. The Epstein-Barr virus was investigated using in situ hybridization with the EBER1 probe. Immunohistochemical studies were performed on paraffin sections. Cases from both adult and pediatric age groups were included. Correlations with histologic subtype, clinicopathologic features, and immunophenotype were determined. Results. Epstein-Barr virus sequences were identified in 18/27 (67%) cases. Positivity correlated with histologic subtype: 0/1 lymphocyte predominant; 6/13 (46%) nodular sclerosis; 7/7 mixed cellularity (MC) (100%); and 5/6 (83%) lymphocyte depleted (LD), The proportion of cases classified as MC and LD (13 of 27) was greater than that found in the United States and other developed countries. The immunophenotypic profile was appropriate for Hodgkin's disease, with all cases of classic Hodgkin's disease positive for CD30 (Ber-H2) and 18 cases expressing CD15. One case of lymphocyte-predominant Hodgkin's disease was CD20 (L26)-positive as were three cases of classic Hodgkin's disease. Patient age ranged from 5 to 65 years, with a median of 29 years. Conclusions. The EBV is associated highly with HD in Mexico, and this prevalence rate is found in all age groups. A strong correlation between EBV expression and histologic subtype was confirmed, with 92% of MC and LD subtypes found to be positive. C1 HOSP CENT MIL,DEPT PATHOL,MEXICO CITY,DF,MEXICO. NCI,HEMATOPATHOL SECT,BETHESDA,MD 20892. RP ZARATEOSORNO, A (reprint author), INST NACL CANCEROL,DIV CLIN INVEST,AVE SAN FERNANDO 22,MEXICO CITY 14000,DF,MEXICO. NR 45 TC 39 Z9 40 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD MAR 15 PY 1995 VL 75 IS 6 BP 1360 EP 1366 DI 10.1002/1097-0142(19950315)75:6<1360::AID-CNCR2820750619>3.0.CO;2-U PG 7 WC Oncology SC Oncology GA QL728 UT WOS:A1995QL72800018 PM 7882287 ER PT J AU EYRE, H SONDIK, E SMITH, RA KESSLER, L AF EYRE, H SONDIK, E SMITH, RA KESSLER, L TI JOINT MEETING ON THE FEASIBILITY OF A STUDY OF SCREENING PREMENOPAUSAL WOMEN (40-49 YEARS) FOR BREAST-CANCER - APRIL 20-21, 1994 SO CANCER LA English DT Editorial Material C1 NCI,BETHESDA,MD 20892. RP EYRE, H (reprint author), AMER CANC SOC,1599 CLIFTON RD NE,ATLANTA,GA 30329, USA. NR 1 TC 12 Z9 12 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD MAR 15 PY 1995 VL 75 IS 6 BP 1391 EP 1403 DI 10.1002/1097-0142(19950315)75:6<1391::AID-CNCR2820750623>3.0.CO;2-4 PG 13 WC Oncology SC Oncology GA QL728 UT WOS:A1995QL72800022 PM 7882290 ER PT J AU SEKIDO, Y PASS, HI BADER, S MEW, DJY CHRISTMAN, MF GAZDAR, AF MINNA, JD AF SEKIDO, Y PASS, HI BADER, S MEW, DJY CHRISTMAN, MF GAZDAR, AF MINNA, JD TI NEUROFIBROMATOSIS TYPE-2 (NF2) GENE IS SOMATICALLY MUTATED IN MESOTHELIOMA BUT NOT IN LUNG-CANCER SO CANCER RESEARCH LA English DT Note ID FREE DEFINED MEDIUM; MALIGNANT MESOTHELIOMA; CELL-LINES; CLINICAL SPECIMENS; CHROMOSOME CHANGES; TUMOR SUPPRESSOR; ABNORMALITIES; PROTEIN; GROWTH AB We have found 16 of 28 small cell lung cancers, 17 of 31 non-small cell lung cancers, 2 of 3 carcinoids, and 12 of 14 mesotheliomas that had chromosome 22 cytogenetic abnormalities. To determine whether the neurofibromatosis type 2 (NF2) gene located on chromosome 22 participates in the oncogenesis of these malignancies, we studied DNAs from lung cancer cell lines and mesotheliomas using Southern blot analysis and the single-strand conformation polymorphism (SSCP) technique for mutations covering 8 of the 16 known NF2 exons. We detected 7 mutations in 17 mesotheliomas (41%) within the coding region of NF2 but none in 75 lung cancer cell lines (38 small cell lung cancers, 34 non-small cell lung cancers, and 3 carcinoids). These mutations were found to be somatic when normal tissue was available for testing. Four mesothelioma cell lines had relatively large deletions (similar to 10-50 kilobases) in the NF2 gene detectable by Southern blot analysis. Two mesothelioma cell lines had nonsense mutations at codons 57 and 341, respectively. Another mesothelioma obtained as a specimen directly from a patient, had a 10-base pair microdeletion from nucleotide 1004 to nucleotide 1013 causing a frameshift mutation. These results suggest that the NF2 gene participates in the oncogenesis in a subset of mesotheliomas but not in lung cancers. C1 UNIV TEXAS,SW MED CTR,SIMMONS CANC CTR,DALLAS,TX 75235. NCI,SURG BRANCH,THORAC ONCOL SECT,BETHESDA,MD 20892. UNIV CALIF SAN FRANCISCO,DEPT RADIAT ONCOL,SAN FRANCISCO,CA 94143. FU NCI NIH HHS [P20 CA58220-01] NR 27 TC 183 Z9 184 U1 0 U2 4 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 15 PY 1995 VL 55 IS 6 BP 1227 EP 1231 PG 5 WC Oncology SC Oncology GA QL405 UT WOS:A1995QL40500007 PM 7882313 ER PT J AU DABHOLKAR, MD BERGER, MS VIONNET, JA EGWUAGU, C SILBER, JR YU, JJ REED, E AF DABHOLKAR, MD BERGER, MS VIONNET, JA EGWUAGU, C SILBER, JR YU, JJ REED, E TI MALIGNANT AND NONMALIGNANT BRAIN-TISSUES DIFFER IN THEIR MESSENGER-RNA EXPRESSION PATTERNS FOR ERCC1 AND ERCC2 SO CANCER RESEARCH LA English DT Article ID EXCISION REPAIR GENE; A XERODERMA-PIGMENTOSUM; DNA-REPAIR; MOLECULAR CHARACTERIZATION; CANCER-PATIENTS; CDNA CLONING; HOMOLOGY; YEAST; CELLS AB Perturbation of the DNA repair process appears to be responsible for the occurrence of a number of human diseases, which are usually associated with a propensity to develop internal malignancies and/or disorders of the central nervous system. We have been interested in the possibility that a subtle abnormality in DNA repair competency might be associated with the transformation of nonmalignant cells to the malignant state. To study this question, we assayed malignant and nonmalignant brain tissues from 19 individuals for mRNA expression levels of the human DNA repair genes ERCC1, ERCC2, and XPAC and for differential splicing of the ERCC1 transcript. We separately compared expression levels of these genes in the following situations: concordance of expression within malignant tissues; concordance of expression within nonmalignant tissues; concordance between malignant and nonmalignant tissues within individuals of the cohort; and concordance of gene expression between two nonmalignant tissue sites within a single individual. Linear regression analyses of mRNA values obtained suggested orderly concordance of these three DNA repair genes in nonmalignant tissues within the patient cohort and an excellent concordance of these genes between two separate biopsy sites from the same individual. In contrast, malignant tissues showed disruption of concordance between the full-length ERCC1 transcript and ERCC2, which have excision and helicase functions, respectively. Furthermore, within the same individuals, malignant tissues were discordant with nonmalignant tissues for ERCC1 and ERCC2, although concordance for XPAC was preserved. These data suggest that one molecular characteristic of human malignancy may be the disruption of the normal relationship between the excision and the helicase functions of the nucleotide excision repair pathway. C1 NCI, CLIN PHARMACOL BRANCH, MED OVARIAN CANC SECT, BETHESDA, MD 20892 USA. UNIV WASHINGTON, MED CTR, DEPT NEUROL SURG, SEATTLE, WA 98195 USA. NEI, IMMUNOL LAB, BETHESDA, MD 20892 USA. NR 27 TC 45 Z9 46 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 15 PY 1995 VL 55 IS 6 BP 1261 EP 1266 PG 6 WC Oncology SC Oncology GA QL405 UT WOS:A1995QL40500014 PM 7882319 ER PT J AU VIOLA, JJ AGBARIA, R WALBRIDGE, S OSHIRO, EM JOHNS, DG KELLEY, JA OLDFIELD, EH RAM, Z AF VIOLA, JJ AGBARIA, R WALBRIDGE, S OSHIRO, EM JOHNS, DG KELLEY, JA OLDFIELD, EH RAM, Z TI IN-SITU CYCLOPENTENYL CYTOSINE INFUSION FOR THE TREATMENT OF EXPERIMENTAL BRAIN-TUMORS SO CANCER RESEARCH LA English DT Article ID TRIPHOSPHATE; ANTITUMOR AB Cyclopentenylcytosine (CPEC; NSC 375575) is a pyrimidine nucleoside analogue that has potent antitumor effects when tested in vitro and also when tested in experimental tumors outside the central nervous system. CPEC exerts its antiproliferative effect through inhibition of CTP synthetase and consequent depletion of CTP and dCTP pools required for cell replication. Due to its poor penetration of the blood-brain barrier, CPEC has failed to demonstrate therapeutic efficacy in experimental brain tumors after systemic administration. We therefore examined the in vivo activation, distribution, and antitumor effect of CPEC after long-term regional infusion of the drug directly into experimental brain tumors in rats. HPLC analysis of CPEC incubated with homogenized human brain and brain tumor tissue showed minimal degradation of the drug over 24 h. Analysis of rat cerebral 9L gliosarcoma infused with tritium-labeled CPEC demonstrated intratunoral accumulation of the active metabolite CPEC-triphosphate and concomitant depletion of CTP to a much greater extent in tumor tissue than in the adjacent brain. Tumor tissue UTP also decreased, but no significant effects on other ribonucleoside hiphosphates were detected. Only trace amounts (<1%) of CPEC and its metabolites reached peripheral sites, including the liver and kidneys, after intratumoral infusion. Rats treated with continuous intratumoral infusion of CPEC for 4 weeks using s.c. implanted osmotic pumps survived significantly longer than control rats receiving intratumoral saline or i.p. CPEC (P < 0.0001). Long-term intratumoral infusion of CPEC was not associated with any detectable toxicity. Our results support the feasibility of using intratumoral administration of CPEC as a regional therapy for malignant brain tumors. C1 NINCDS,SURG NEUROL BRANCH,BETHESDA,MD 20892. NCI,DEV THERAPEUT PROGRAM,BETHESDA,MD 20892. NR 15 TC 27 Z9 29 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 15 PY 1995 VL 55 IS 6 BP 1306 EP 1309 PG 4 WC Oncology SC Oncology GA QL405 UT WOS:A1995QL40500022 PM 7882327 ER PT J AU FUJIMORI, A HARKER, WG KOHLHAGEN, G HOKI, Y POMMIER, Y AF FUJIMORI, A HARKER, WG KOHLHAGEN, G HOKI, Y POMMIER, Y TI MUTATION AT THE CATALYTIC SITE OF TOPOISOMERASE-I IN CEM/C2, A HUMAN LEUKEMIA-CELL LINE RESISTANT TO CAMPTOTHECIN SO CANCER RESEARCH LA English DT Article ID HAMSTER DC3F CELLS; DNA TOPOISOMERASE; DRUG CAMPTOTHECIN; POINT MUTATION; LUNG-CANCER; GENE; IDENTIFICATION; CLONING; CDNA; MECHANISM AB We developed previously a resistant cell line, CEM/C2, from the human leukemia cell line CCRF-CEM by stepwise selection in camptothecin. This cell line is 974-fold more resistant to camptothecin than parental cells. Resistance is only partially explained by 2-fold reductions in topoisomerase I protein and mRNA levels. We further investigated biochemical and molecular features of topoisomerase I in the resistant cell line. Sequence analyses of the top1 cDNA from CEM/C2 identified mutations corresponding to two amino acid substitutions, Met370Thr and Asn722Ser. Asn722Ser is next to the catalytic Tyr723 in a region highly conserved among type I eukargotic DNA topoisomerases. Recombinant top1 with the corresponding substitution was found to be catalytically active and resistant to camptothecin. These results indicate that camptothecin resistance of CEM/C2 is due to the mutation Asn722Ser and strongly suggest that the asparagine immediately flanking the catalytic tyrosine is important for the camptothecin action. C1 NCI, DIV CANC TREATMENT, MOLEC PHARMACOL LAB, BETHESDA, MD 20892 USA. DEPT VET AFFAIRS MED CTR, SALT LAKE CITY, UT 84148 USA. NR 39 TC 147 Z9 150 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD MAR 15 PY 1995 VL 55 IS 6 BP 1339 EP 1346 PG 8 WC Oncology SC Oncology GA QL405 UT WOS:A1995QL40500028 PM 7882333 ER PT J AU PANZA, JA GARCIA, CE KILCOYNE, CM QUYYUMI, AA CANNON, RO AF PANZA, JA GARCIA, CE KILCOYNE, CM QUYYUMI, AA CANNON, RO TI IMPAIRED ENDOTHELIUM-DEPENDENT VASODILATION IN PATIENTS WITH ESSENTIAL-HYPERTENSION - EVIDENCE THAT NITRIC-OXIDE ABNORMALITY IS NOT LOCALIZED TO A SINGLE SIGNAL-TRANSDUCTION PATHWAY SO CIRCULATION LA English DT Article DE ENDOTHELIUM; HYPERTENSION; BRADYKININ; ACETYLCHOLINE; PROTEINS ID PROTEIN-LINKED RECEPTORS; RELAXING FACTOR; VASCULAR RELAXATION; CORONARY-ARTERIES; PULMONARY-ARTERY; SMOOTH-MUSCLE; L-ARGININE; PHOSPHOINOSITIDE TURNOVER; MUSCARINIC RECEPTOR; ALPHA-SUBUNITS AB Background Patients with essential hypertension have abnormal endothelium-dependent vascular relaxation, largely related to reduced bioactivity of nitric oxide (NO). The purpose of the present investigation was to determine whether this defect is due to a deficit at the specific intracellular signal-transduction pathway level or is a consequence of a more generalized endothelial abnormality. Methods and Results The responses of the forearm vasculature to acetylcholine and bradykinin (endothelium-dependent agents that act through different signal transduction pathways) and to sodium nitroprusside (a direct dilator of vascular smooth muscle) were studied in 10 hypertensive patients (5 men, 5 women; aged 48+/-9 years old [mean+/-SD]) and 12 control subjects (6 men, 6 women; aged 48+/-7 years old). To determine the contribution of NO to bradykinin-induced vasodilation, the vascular responses to bradykinin were also measured after administration of N-G-monomethyl-L-arginine, an arginine analogue that inhibits the synthesis of NO. Drugs were infused into the brachial artery, and forearm blood flow was measured by strain-gauge plethysmography. The response to acetylcholine was significantly blunted in hypertensive patients (maximal blood flow, 7.5+/-2 versus 16.6+/-8 mL . min(-1). 100 mL(-1) in control subjects [mean+/-SD]; P<.005). Similarly, the vasodilator effect of bradykinin was significantly reduced in hypertensive patients compared with control subjects (maximal blood flow, 8.7+/-2 versus 15.8+/-6 mL . min(-1). 100 mL(-1) in control subjects; P<.005). A significant correlation was found between the maximal blood flow with acetylcholine and that with bradykinin (r=.89). No significant differences were found between the two groups for vascular response to sodium nitroprusside. N-G-monomethyl-L-arginine significantly blunted the response to bradykinin in control subjects (maximal blood flow decreased from 15.8+/-6 to 10.1+/-2 mL . min(-1). 100 mL(-1), P<.003). In contrast, inhibition of NO synthesis did not modify the response to bradykinin in hypertensive patients (maximal blood flow, 8.7+/-2 and 8.5+/-3 before and during infusion of N-G-monomethyl-L-arginine, respectively; P=NS). As a consequence, the response to bradykinin after inhibition of NO synthesis was not significantly different between the two groups. Conclusions Patients with essential hypertension have impaired endothelium-dependent vasodilator responses to both acetylcholine and bradykinin. These findings indicate that the endothelial dysfunction in this condition is not related to a specific defect of a single intracellular signal-transduction pathway and suggest a more generalized abnormality of endothelial vasodilator function. RP PANZA, JA (reprint author), NHLBI, CARDIOL BRANCH, BLDG 10, ROOM 7B-15, BETHESDA, MD 20892 USA. NR 58 TC 275 Z9 286 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7322 J9 CIRCULATION JI Circulation PD MAR 15 PY 1995 VL 91 IS 6 BP 1732 EP 1738 PG 7 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA QL458 UT WOS:A1995QL45800017 PM 7882481 ER PT J AU GARDIN, JM SISCOVICK, D ANTONCULVER, H LYNCH, JC SMITH, VE KLOPFENSTEIN, HS BOMMER, WJ FRIED, L OLEARY, D MANOLIO, TA AF GARDIN, JM SISCOVICK, D ANTONCULVER, H LYNCH, JC SMITH, VE KLOPFENSTEIN, HS BOMMER, WJ FRIED, L OLEARY, D MANOLIO, TA TI SEX, AGE, AND DISEASE AFFECT ECHOCARDIOGRAPHIC LEFT-VENTRICULAR MASS AND SYSTOLIC FUNCTION IN THE FREE-LIVING ELDERLY - THE CARDIOVASCULAR HEALTH STUDY SO CIRCULATION LA English DT Article DE ECHOCARDIOGRAPHY; MULTICENTER STUDY; AGING; CARDIOVASCULAR DISEASES; VENTRICLES ID HEART-DISEASE; POPULATION; ADULTS; IMPACT AB Background Left ventricular (LV) hypertrophy, as measured by M-mode echocardiography, is an independent predictor of mortality and/or morbidity from coronary heart disease (CHD). LV global and segmental systolic dysfunction also have been associated with myocardial ischemia and cardiovascular morbidity and mortality. Echocardiographic data, especially two-dimensional, have not been available previously from multicenter-based studies of the elderly. This report describes the distribution and relation at baseline of echocardiographic LV mass and global and segmental LV wall motion to age, sex, and clinical disease category in the Cardiovascular Health Study (CHS), a cohort of 5201 men and women (4850 white) 65 years of age and older. Methods and Results M-mode LV mass adjusted for body weight increased modestly with age (P<.0001), increasing less than one gram per year increase in age for both men and women. After adjustment for weight, LV mass was significantly greater in men than in women and in participants with clinical CHD compared with participants with neither clinical heart disease nor hypertension (both P<.001). Across all CHS age subgroups, the difference in weight-adjusted LV mass by sex was greater in magnitude than the difference related to clinical CHD. M-mode measurements of LV mass could not be made in 34% of CHS participants, and this was highly related to age (29% in the 65 to 69 year versus 50% in the 85+ year age group, P<.001) and other risk factors. In participants with clinical CHD and with neither clinical heart disease nor hypertension, LV ejection fraction and segmental wall motion abnormalities were more prevalent in men than women (all P<.001). Of interest, 0.5% of men and 0.4% of women with neither clinical heart disease nor hypertension had LV segmental wall motion abnormalities, suggesting silent disease, compared with 26% of men and 10% of women in the clinical CHD group (P<.0001). Multivariate analyses revealed male sex and presence of clinical CHD (both P<.001) to be independent predictors of LV akinesis or dyskinesis. Conclusions Significant baseline relations were detected between differences in sex, prevalent disease status, and echocardiographic measurements of LV mass and systolic function in the CHS cohort. Age was weakly associated with LV mass measurements and LV ejection fraction abnormalities. These relations should be considered in evaluating the preclinical and clinical effects of CHD risk factors in the elderly. C1 UNIV CALIF IRVINE,DEPT MED,DIV CARDIOL,IRVINE,CA. UNIV CALIF IRVINE,DEPT MED,DIV EPIDEMIOL,IRVINE,CA. UNIV WASHINGTON,HARBORVIEW MED CTR,DEPT MED,SEATTLE,WA. UNIV WASHINGTON,HARBORVIEW MED CTR,DEPT EPIDEMIOL,SEATTLE,WA. UNIV WASHINGTON,HARBORVIEW MED CTR,DEPT BIOSTAT,SEATTLE,WA. ALBANY MED COLL,DIV CARDIOL,ALBANY,NY. BOWMAN GRAY SCH MED,DEPT MED,DIV CARDIOL,WINSTON SALEM,NC. UNIV CALIF DAVIS,DEPT MED,DIV CARDIOL,SACRAMENTO,CA. JOHNS HOPKINS MED INST,DEPT MED,BALTIMORE,MD. JOHNS HOPKINS MED INST,DEPT EPIDEMIOL,BALTIMORE,MD. GEISINGER MED CTR,DEPT RADIOL,DANVILLE,PA. NHLBI,DIV EPIDEMIOL & CLIN APPLICAT,BETHESDA,MD. FU NHLBI NIH HHS [HC-85086, N01-HC85079] NR 24 TC 181 Z9 185 U1 0 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD MAR 15 PY 1995 VL 91 IS 6 BP 1739 EP 1748 PG 10 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA QL458 UT WOS:A1995QL45800018 PM 7882482 ER PT J AU YAMAGUCHIIWAI, Y DANCIS, A KLAUSNER, RD AF YAMAGUCHIIWAI, Y DANCIS, A KLAUSNER, RD TI AFT1 - A MEDIATOR OF IRON-REGULATED TRANSCRIPTIONAL CONTROL IN SACCHAROMYCES-CEREVISIAE SO EMBO JOURNAL LA English DT Article DE FERRIC REDUCTASE; IRON; TRANSCRIPTION; TRANSPORT ID ELEMENT BINDING-PROTEIN; FERRIC REDUCTASE; MOLECULAR CHARACTERIZATION; SIDEROPHORE BIOSYNTHESIS; USTILAGO-MAYDIS; MAMMALIAN-CELLS; GENE; YEAST; RNA; EXPRESSION AB Using a scheme for selecting mutants of Saccharomyces cerevisiae with abnormalities of iron metabolism, we have identified a gene, AFT1, that mediates the control of iron uptake. AFT1 encodes a 78 kDa protein with a highly basic amino terminal domain and a glutamine-rich C-terminal domain, reminiscent of transcriptional activators. The protein also contains an amino terminal and a C-terminal region with 10% His residues. A dominant mutant allele of this gene, termed AFT1-1(up), results in high levels of ferric reductase and ferrous iron uptake that are not repressed by exogenous iron. The increased iron uptake is associated with enhanced susceptibility to iron toxicity. These effects may be explained by the failure of iron to repress transcription of FRE1, FRE2 and FET3. FRE1 and FRE2 encode plasma membrane ferric reductases, obligatory for ferric iron assimilation, and FET3 encodes a copper-dependent membrane-associated oxidase required for ferrous iron uptake. Conversely, a strain with interruption of the AFT1 gene manifests low ferric reductase and ferrous iron uptake and is susceptible to iron deprivation, because of deficient expression of FRE1 and negligible expression of FRE2 and FET3. Thus, AFT1 functions to activate transcription of target genes in response to iron deprivation and thereby plays a central role in iron homeostasis. C1 NICHHD, CELL BIOL & METAB BRANCH, BETHESDA, MD 20892 USA. NR 41 TC 223 Z9 232 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0261-4189 EI 1460-2075 J9 EMBO J JI Embo J. PD MAR 15 PY 1995 VL 14 IS 6 BP 1231 EP 1239 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QR037 UT WOS:A1995QR03700019 PM 7720713 ER PT J AU GARNER, MM RAU, DC AF GARNER, MM RAU, DC TI WATER RELEASE ASSOCIATED WITH SPECIFIC BINDING OF GAL REPRESSOR SO EMBO JOURNAL LA English DT Article DE GAL REPRESSOR; HYDRATION; OSMOTIC STRESS; PROTEIN-DNA INTERACTIONS ID PROTEIN-DNA INTERACTIONS; MOBILITY-SHIFT ASSAY; STABILIZATION; SOLVATION; ELEMENTS; INVITRO; COMPLEX AB Water release coupled to the association of gal repressor with DNA is measured from the sensitivity of the binding constant to the solution osmotic pressure, using neutral solutes that are typically excluded from polar protein and DNA surfaces. Differences in water release for binding of repressor to different sequences are linked with differences in specificity and binding energies. With sucrose, the specific binding of repressor to operator sequences is accompanied by the release of 130 water molecules. No water release is seen for the weak, non-specific binding of repressor to poly(dI-dC).(dI-dC). A difference in the release of six water molecules is seen even for the binding of gal repressor to two different operator sequences that differ in affinity by only a factor of two. C1 NIDDK,OFF INTRAMURAL RES,BETHESDA,MD 20892. NICHHD,THEORET & PHYS BIOL LAB,BETHESDA,MD 20892. NR 33 TC 87 Z9 89 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0261-4189 J9 EMBO J JI Embo J. PD MAR 15 PY 1995 VL 14 IS 6 BP 1257 EP 1263 PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QR037 UT WOS:A1995QR03700022 PM 7720716 ER PT J AU ANGLADE, E SULLIVAN, D NUSSENBLATT, R CSAKY, K AF ANGLADE, E SULLIVAN, D NUSSENBLATT, R CSAKY, K TI PRODUCTION OF AN IMMUNOADHESIN WITH ANTAGONIST ACTIVITY TOWARDS THE MURINE INTERLEUKIN-2 RECEPTOR SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,IMMUNOL LAB,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S542 EP S542 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502515 ER PT J AU AYYAGARI, R SMITH, RJH POLYJMEROPOLOUS, M DAIGER, S PELIAS, MZ WOZENCRAFT, L KAISERKUPFER, M NESTOROWICZ, A PERMUTT, A LEE, Y HEJTMANCIK, JF AF AYYAGARI, R SMITH, RJH POLYJMEROPOLOUS, M DAIGER, S PELIAS, MZ WOZENCRAFT, L KAISERKUPFER, M NESTOROWICZ, A PERMUTT, A LEE, Y HEJTMANCIK, JF TI A YAC CONTIG ENCOMPASSING THE USHIC LOCUS ON THE SHORT ARM OF CHROMOSOME-11 SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. UNIV IOWA,DEPT OTOLARYNGOL,IOWA CITY,IA 52242. NIH,NATL CTR HUMAN GENOME RES,BETHESDA,MD 20892. UNIV TEXAS,HLTH SCI CTR,HOUSTON,TX 77225. LOUISIANA STATE UNIV,MED CTR,DEPT BIOMETRY & GENET,NEW ORLEANS,LA 70112. WASHINGTON UNIV,DEPT INTERNAL MED,ST LOUIS,MO 63130. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S1045 EP S1045 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504822 ER PT J AU BERNSTEIN, SL BORST, DE WONG, P AF BERNSTEIN, SL BORST, DE WONG, P TI CHARACTERIZATION OF A HUMAN FOVEAL PRIMARY CDNA LIBRARY AND ISOLATION OF CANDIDATE GENES FOR MACULAR DYSTROPHIES SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S621 EP S621 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502852 ER PT J AU BLAKE, DA YU, H NEGRE, E ROBERTS, DD VOGEL, T AF BLAKE, DA YU, H NEGRE, E ROBERTS, DD VOGEL, T TI INTEGRIN-DEPENDENT MIGRATION OF HUMAN AND BOVINE CORNEAL ENDOTHELIAL-CELLS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 TULANE UNIV,SCH MED,DEPT OPHTHALMOL,NEW ORLEANS,LA 70112. NCI,PATHOL LAB,BETHESDA,MD 20892. BIOTECHNOL GEN LTD,REHOVOT,ISRAEL. RI Roberts, David/A-9699-2008 OI Roberts, David/0000-0002-2481-2981 NR 1 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S294 EP S294 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91501353 ER PT J AU BOATRIGHT, JH BORST, DE LIN, ZY SI, JS BHATI, M PATEL, D BRUNO, J NICKERSON, JM AF BOATRIGHT, JH BORST, DE LIN, ZY SI, JS BHATI, M PATEL, D BRUNO, J NICKERSON, JM TI FUNCTIONAL-CHARACTERIZATION OF CIS-ACTING REGULATORY ELEMENTS OF THE IRBP GENE PROMOTER REGION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 EMORY UNIV,SCH MED,ATLANTA,GA 30322. NEI,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S123 EP S123 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500599 ER PT J AU BORRAS, T ZIGLER, JS AF BORRAS, T ZIGLER, JS TI ADENOVIRUS-MEDIATED GENE-TRANSFER INTO WHOLE MONKEY LENS IN ORGAN-CULTURE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,MECHANISMS OCULAR DIS LAB,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S844 EP S844 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503872 ER PT J AU CARPER, DA OLD, SE ELKABBANI, O HOHMAN, TC AF CARPER, DA OLD, SE ELKABBANI, O HOHMAN, TC TI STRUCTURE-FUNCTION STUDIES OF HUMAN ALDOSE REDUCTASE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 WYETH AVERST LAB,PRINCETON,NJ. NEI,BETHESDA,MD 20892. UNIV ALABAMA,BIRMINGHAM,AL 35294. NR 2 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S821 EP S821 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503792 ER PT J AU CARUSO, RC KAISERKUPFER, MI GOODMAN, LA ZUJEWSKI, JA OSHAUGNESSY, JA AF CARUSO, RC KAISERKUPFER, MI GOODMAN, LA ZUJEWSKI, JA OSHAUGNESSY, JA TI EFFECTS OF FENRETINIDE (4-HPR) ON DARK-ADAPTATION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S923 EP S923 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504236 ER PT J AU CHAN, CC FACTOR, V LI, Q NAGY, P PENG, B THORGEIRSSON, S AF CHAN, CC FACTOR, V LI, Q NAGY, P PENG, B THORGEIRSSON, S TI THE EYES OF TGF-BETA-1 TRANSGENIC MICE - HISTOLOGY AND IMMUNE-RESPONSES SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,IMMUNOL LAB,BETHESDA,MD 20892. NCI,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S202 EP S202 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500933 ER PT J AU CHEN, PW SALGALLER, ML ROSENBERG, SA MURRAY, T KSANDER, BR AF CHEN, PW SALGALLER, ML ROSENBERG, SA MURRAY, T KSANDER, BR TI INCREASED EXPRESSION OF GENES ENCODING TUMOR-SPECIFIC ANTIGENS IN OCULAR MELANOMA - POTENTIAL TARGETS FOR IMMUNOTHERAPY SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 SCHEPENS EYE RES INST,BOSTON,MA. NCI,SURG BRANCH,BETHESDA,MD 20892. UNIV MIAMI,DEPT OPHTHALMOL,MIAMI,FL 33152. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S221 EP S221 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91501008 ER PT J AU CHEPELINSKY, AB PARKERWILSON, DM KUANG, K FISCHBARG, J AF CHEPELINSKY, AB PARKERWILSON, DM KUANG, K FISCHBARG, J TI A NEW WATER CHANNEL EXPRESSED IN CORNEAL ENDOTHELIAL-CELLS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. COLUMBIA UNIV COLL PHYS & SURG,NEW YORK,NY 10032. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S7 EP S7 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500031 ER PT J AU CHEUNG, MK WALTON, RC CHAN, CC PARKS, DJ WHITCUP, SM NUSSENBLATT, RB AF CHEUNG, MK WALTON, RC CHAN, CC PARKS, DJ WHITCUP, SM NUSSENBLATT, RB TI SUBRETINAL FIBROSIS IN VOGT-KOYANAGI-HARADA SYNDROME SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,IMMUNOL LAB,BETHESDA,MD 20892. NEI,CLIN BRANCH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S782 EP S782 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503604 ER PT J AU CHOUDHURY, A CHALAM, KV PAKALNIS, VA CASPI, RR BOWERS, WE AF CHOUDHURY, A CHALAM, KV PAKALNIS, VA CASPI, RR BOWERS, WE TI PROCESSING AND PRESENTATION OF S-ANTIGEN BY RAT CHOROIDAL DENDRITIC CELLS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV S CAROLINA,SCH MED,DEPT MICROBIOL & IMMUNOL,COLUMBIA,SC 29208. UNIV S CAROLINA,SCH MED,DEPT OPHTHALMOL,COLUMBIA,SC 29208. NEI,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S543 EP S543 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502520 ER PT J AU CHRISTOFORIDIS, JB CARUSO, RC CHOI, R KAISERKUPFER, MI AF CHRISTOFORIDIS, JB CARUSO, RC CHOI, R KAISERKUPFER, MI TI THE VOLUME OF THE VISUAL-FIELD ASSESSED WITH KINETIC PERIMETRY SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OPHTHALM GENET & CLIN SERV BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S451 EP S451 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502071 ER PT J AU CHUNG, H LI, Q WHITCUP, SM NUSSENBLATT, RB CHAN, CC AF CHUNG, H LI, Q WHITCUP, SM NUSSENBLATT, RB CHAN, CC TI EXPRESSION OF TGF-BETA-1 MESSENGER-RNA ON IRIDECTOMY SPECIMENS FROM PATIENTS WITH UVEITIS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. SEOUL NATL UNIV,COLL MED,DEPT OPHTHALMOL,SEOUL,SOUTH KOREA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S101 EP S101 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500486 ER PT J AU CORDAHI, GJ PEARSON, PA MARTIN, DF SCHMEISSER, ET NUSSENBLATT, RB ASHTON, P AF CORDAHI, GJ PEARSON, PA MARTIN, DF SCHMEISSER, ET NUSSENBLATT, RB ASHTON, P TI TOXICITY OF SUSTAINED-RELEASE CYCLOSPORINE-A IN NONHUMAN PRIMATE EYES SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV KENTUCKY,DEPT OPHTHALMOL,LEXINGTON,KY 40506. EMORY UNIV,DEPT OPHTHALMOL,ATLANTA,GA 30322. NEI,BETHESDA,MD 20892. TUFTS UNIV,DEPT OPHTHALMOL,BOSTON,MA 02111. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S539 EP S539 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502501 ER PT J AU COURTNEY, SM CLARK, VP KARNI, A MARTIN, A UNGERLEIDER, LG HAXBY, JV AF COURTNEY, SM CLARK, VP KARNI, A MARTIN, A UNGERLEIDER, LG HAXBY, JV TI FMRI STUDIES REVEAL THAT ATTENTION, WORKING-MEMORY, AND LEARNING MODULATE ACTIVITY IN HUMAN VISUAL NEURAL SYSTEMS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NIMH,BETHESDA,MD 20892. RI martin, alex/B-6176-2009 NR 0 TC 1 Z9 1 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S612 EP S612 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502823 ER PT J AU CRABBE, MJC CHEPELINSKY, AB DILSIZ, N AF CRABBE, MJC CHEPELINSKY, AB DILSIZ, N TI IN-VIVO INSERTION OF RAT LENS MIP INTO THE ESCHERICHIA-COLI CYTOPLASMIC MEMBRANE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV READING,DEPT MICROBIOL,READING RG6 2AH,BERKS,ENGLAND. NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S198 EP S198 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500920 ER PT J AU DASTGHEIB, K LI, Q CHAN, CC ROBERGE, FG CSAKY, K GREEN, WR AF DASTGHEIB, K LI, Q CHAN, CC ROBERGE, FG CSAKY, K GREEN, WR TI VASCULAR ENDOTHELIAL GROWTH-FACTOR (VEGF) IN NEOVASCULAR AGE-RELATED MACULAR DEGENERATION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. GRAD HOSP PHILADELPHIA,PHILADELPHIA,PA 19146. JOHNS HOPKINS MED INST,WILNER OPHTHALMOL INST,BALTIMORE,MD 21205. NR 0 TC 8 Z9 8 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S102 EP S102 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500493 ER PT J AU DEREVIANIK, NL VINORES, SA PENG, B MAHLOW, J CHIU, C CAMPOCHIARO, PA CHAN, CC AF DEREVIANIK, NL VINORES, SA PENG, B MAHLOW, J CHIU, C CAMPOCHIARO, PA CHAN, CC TI EFFECTS OF CYCLOSPORINE-A (CSA), DEXAMETHASONE, AND THROMBOXANE SYNTHETASE INHIBITOR (CGS-13080) ON BLOOD-RETINAL BARRIER BREAKDOWN IN EXPERIMENTAL AUTOIMMUNE UVEORETINITIS - AN ULTRASTRUCTURAL-STUDY SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,SCH MED,WILMER OPHTHALMOL INST,BALTIMORE,MD 21205. NEI,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S544 EP S544 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502524 ER PT J AU DILLON, J GAILLARD, EA BILSKI, P CHIGNELL, C RESZKA, K AF DILLON, J GAILLARD, EA BILSKI, P CHIGNELL, C RESZKA, K TI THE PHOTOCHEMISTRY OF THE RETINOIDS AS STUDIED BY STEADY-STATE AND PULSED METHODS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 COLUMBIA UNIV,DEPT OPHTHALMOL,NEW YORK,NY 10027. UNIV ROCHESTER,DEPT CHEM,ROCHESTER,NY 14627. NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S124 EP S124 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500603 ER PT J AU DUNCAN, T PALMER, K CHADER, GJ WIGGERT, B AF DUNCAN, T PALMER, K CHADER, GJ WIGGERT, B TI A PUTATIVE RETINAL-PIGMENT EPITHELIAL-CELL SURFACE-RECEPTOR FOR INTERPHOTORECEPTOR RETINOID-BINDING PROTEIN (IRBP) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,RETINAL CELL & MOLEC BIOL LAB,BETHESDA,MD 20892. NR 0 TC 3 Z9 3 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S122 EP S122 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500593 ER PT J AU EGWUAGU, CE OHTAKAMARAYUMA, C MAHDI, R SMITH, J CHEPELINSKY, AB AF EGWUAGU, CE OHTAKAMARAYUMA, C MAHDI, R SMITH, J CHEPELINSKY, AB TI CONSTITUTIVE SYNTHESIS OF GAMMA-INTERFERON IN THE LENS OF TRANSGENIC MICE ALTERS THE PATTERN OF LENS GENE-EXPRESSION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S879 EP S879 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504016 ER PT J AU FERRIS, FL AF FERRIS, FL TI ANTIOXIDANTS AND THE EYE - CLINICAL-TRIALS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,DIV BIOMETRY & EPIDEMIOL,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S193 EP S193 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500901 ER PT J AU FONG, DS MYERS, FL SEGAL, PP HUBBARD, LM DAVIS, MD FERRIS, FL AF FONG, DS MYERS, FL SEGAL, PP HUBBARD, LM DAVIS, MD FERRIS, FL TI SUBRETINAL FIBROSIS IN PATIENTS WITH DIABETIC-RETINOPATHY SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV WISCONSIN,CTGR FUNDUS PHOTOGRAPH READING,MADISON,WI 53706. NEI,CLIN TRIALS BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S819 EP S819 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503786 ER PT J AU FREDERIKSE, P PIATIGORSKY, J AF FREDERIKSE, P PIATIGORSKY, J TI H2O2 AND UV STRESS INDUCTION OF AP-1 BINDING IN VERTEBRATE LENSES SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,LMDB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S843 EP S843 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503869 ER PT J AU FUKUSHIMA, A LAI, JC SHILOACH, J GUEZCROSIER, Y WHITCUP, SM NUSSENBLATT, RB GERY, I AF FUKUSHIMA, A LAI, JC SHILOACH, J GUEZCROSIER, Y WHITCUP, SM NUSSENBLATT, RB GERY, I TI PROMISCUOUS EPITOPES OF HUMAN S-ANTIGEN (H-SAG) STIMULATE LYMPHOCYTES WITH DIFFERENT MHC RESTRICTIONS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NIDDK,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S543 EP S543 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502519 ER PT J AU FUREYKURKJIAN, M PIETRINI, P GRAFFRADFORD, NR ALEXANDER, GE FREO, U GRADY, CL DANI, A MENTIS, M SZCZEPANIK, J HODOS, W SCHAPIRO, MB AF FUREYKURKJIAN, M PIETRINI, P GRAFFRADFORD, NR ALEXANDER, GE FREO, U GRADY, CL DANI, A MENTIS, M SZCZEPANIK, J HODOS, W SCHAPIRO, MB TI CORTICAL VISUAL IMPAIRMENT AS AN EARLY AND PROMINENT SIGN IN ALZHEIMER-DISEASE (AD) - A NEUROPSYCHOLOGICAL AND POSITRON EMISSION TOMOGRAPHY (PET) STUDY SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NIA,NEUROSCI LAB,BETHESDA,MD 20892. MAYO CLIN,DEPT NEUROL,JACKSONVILLE,FL. UNIV MARYLAND,DEPT PSYCHOL,COLLEGE PK,MD 20742. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S682 EP S682 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503122 ER PT J AU GARLAND, DL DUGLASTABOR, Y DATILES, MB ZIGLER, JS MAGNO, B AF GARLAND, DL DUGLASTABOR, Y DATILES, MB ZIGLER, JS MAGNO, B TI ANALYSIS OF HUMAN LENS PROTEINS DURING FIBER CELL MATURATION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 2 Z9 3 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S822 EP S822 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503795 ER PT J AU GEORGE, RK VISTICA, BP NUSSENBLATT, RB WHITCUP, SM AF GEORGE, RK VISTICA, BP NUSSENBLATT, RB WHITCUP, SM TI HIGH SERUM LEVELS OF SOLUBLE ICAM-1 (CD54) IN UVEITIS PATIENTS PREDICT UNDERLYING SYSTEMIC-DISEASE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S536 EP S536 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502489 ER PT J AU GOMES, JAP SCHWARTING, R RIZZO, L ARBIZO, V DONOSO, LA DUA, HS AF GOMES, JAP SCHWARTING, R RIZZO, L ARBIZO, V DONOSO, LA DUA, HS TI MECHANISM OF EXPRESSION OF THE HUMAN MUCOSAL LYMPHOCYTE ANTIGEN (HML-1) IN CONJUNCTIVA ASSOCIATED LYMPHOID-TISSUE (CALT) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 DEPT SANTA CASA OPHTHALMOL,SANTA CASA,SP,BRAZIL. WILLS EYE HOSP & RES INST,PHILADELPHIA,PA 19107. THOMAS JEFFERSON UNIV,PHILADELPHIA,PA 19107. UNIV NOTTINGHAM,DEPT OPHTHALMOL,NOTTINGHAM NG7 2RD,ENGLAND. NEI,BETHESDA,MD 20892. RI Rizzo, Luiz Vicente/B-4458-2009 NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S840 EP S840 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503857 ER PT J AU GONZALEZFERNANDEZ, I BAKER, EL BAER, C OKAJIMA, TIL WIGGERT, B PEPPERBERG, DR AF GONZALEZFERNANDEZ, I BAKER, EL BAER, C OKAJIMA, TIL WIGGERT, B PEPPERBERG, DR TI RECOMBINANT IRBP 4TH REPEAT SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract ID RETINOID-BINDING PROTEIN; INTERPHOTORECEPTOR C1 UNIV VIRGINIA,DEPT OPHTHALMOL,CHARLOTTESVILLE,VA 22903. UNIV ILLINOIS,DEPT OPHTHALMOL & VISUAL SCI,URBANA,IL 61801. NEI,BETHESDA,MD 20892. NR 4 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S6 EP S6 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500027 ER PT J AU GOPALSRIVASTAVA, R HAYNES, J PIATIGORSKY, J AF GOPALSRIVASTAVA, R HAYNES, J PIATIGORSKY, J TI REGULATION OF THE MURINE ALPHA-B-CRYSTALLIN GENE IN CARDIAC-MUSCLE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S880 EP S880 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504023 ER PT J AU GUEXCROSIER, Y WITTWER, AJ ROBERGE, FG AF GUEXCROSIER, Y WITTWER, AJ ROBERGE, FG TI CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANT (CINC) IN ENDOTOXIN-INDUCED UVEITIS (EIU) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. MONSANTO CO,CORP RES,ST LOUIS,MO 63166. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S385 EP S385 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91501808 ER PT J AU HAYNES, JI MCDERMOTT, JB PIATIGORSKY, J AF HAYNES, JI MCDERMOTT, JB PIATIGORSKY, J TI PROMOTER ANALYSIS OF THE CHICKEN BETA-A3/A1-CRYSTALLIN GENE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,LMDB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S881 EP S881 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504031 ER PT J AU HESS, HH ANZANO, MA AF HESS, HH ANZANO, MA TI POSTERIOR SUBCAPSULAR CATARACTS IN RATS TREATED WITH N-METHYL-N-NITROSOUREA SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NCI,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S605 EP S605 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502796 ER PT J AU HOOKS, JJ KOMURASAKI, Y DETRICK, B AF HOOKS, JJ KOMURASAKI, Y DETRICK, B TI EXPRESSION OF VIRAL-RNA, VIRAL-PROTEINS, AND MHC MOLECULES WITHIN RPE AND CILIARY BODY EPITHELIUM DURING CORONAVIRUS INDUCED RETINOPATHY SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. GEORGE WASHINGTON UNIV,MED CTR,WASHINGTON,DC 20037. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S145 EP S145 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500700 ER PT J AU HOPE, JN CHAMBERS, C RUSSELL, P LEE, L HEJTMANCIK, JF AF HOPE, JN CHAMBERS, C RUSSELL, P LEE, L HEJTMANCIK, JF TI FUNCTION OF N-TERMINAL EXTENSION IN BETA-B2-CRYSTALLIN OLIGOMERIZATION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S886 EP S886 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504058 ER PT J AU ILAGAN, JG CVEKL, A KANTOROW, M PIATIGORSKY, J SAX, CM AF ILAGAN, JG CVEKL, A KANTOROW, M PIATIGORSKY, J SAX, CM TI MEMBERS OF THE AP1 FAMILY INTERACT WITH A DOWNSTREAM ELEMENT, PE2, IN THE MOUSE ALPHA-A-CRYSTALLIN PROMOTER SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NIH RES SCHOLARS PROGRAM,HHMI,BETHEWSDA,MD. NEI,MOLEC & DEV BIOL LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S882 EP S882 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504033 ER PT J AU IWATA, F WOZENCRAFT, LA CARUSO, RC LI, A GAHL, WA MCCAIN, LM KAISERKUPFER, MI AF IWATA, F WOZENCRAFT, LA CARUSO, RC LI, A GAHL, WA MCCAIN, LM KAISERKUPFER, MI TI NEPHROPATHIC CYSTINOSIS - NATURAL-HISTORY OF OCULAR FINDINGS AND RESULTS OF CLINICAL-TRIAL OF CYSTEAMINE EYE DROP INTERVENTION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NICHHD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S1056 EP S1056 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504860 ER PT J AU IWATA, T CARPER, DA AF IWATA, T CARPER, DA TI CHARACTERIZATION OF THE HUMAN SORBITOL DEHYDROGENASE GENE PROMOTER SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S879 EP S879 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504013 ER PT J AU JACOT, JL GLOVER, JP BANIK, R FELDMAN, BL ROBISON, WG AF JACOT, JL GLOVER, JP BANIK, R FELDMAN, BL ROBISON, WG TI NOVEL IMPROVEMENTS IN GOLD CHLORIDE PROCEDURES FOR OPTIMAL PRESERVATION OF NERVE STAINING IN WHOLE MOUNTS OF RAT CORNEAS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,PATHOPHYSIOL SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S575 EP S575 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502666 ER PT J AU JIAO, X LEE, J CHADER, GJ AF JIAO, X LEE, J CHADER, GJ TI CLONING AND CHARACTERIZATION OF THE RAT GENE ENCODING RETINAL FATTY-ACID-BINDING PROTEIN (R-FABP) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S621 EP S621 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502855 ER PT J AU KADOR, PF SECCHI, EF LIZAK, MJ SATO, S AF KADOR, PF SECCHI, EF LIZAK, MJ SATO, S TI COMPARISON OF POLYOL PATHWAY FLUX IN LENS, RETINA AND NERVE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OCULAR THERAPEUT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S886 EP S886 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504057 ER PT J AU KIKUCHI, T WAWROUSEK, E LEE, R DICAMILLO, S KUSUZAKI, K SHINOHARA, T AF KIKUCHI, T WAWROUSEK, E LEE, R DICAMILLO, S KUSUZAKI, K SHINOHARA, T TI POSITION OR NUMBER OF PHOTORECEPTOR CONSERVED ELEMENT (PCE1) IN ARRESTIN PROMOTER MAY BE ALTERED IN MOLECULAR EVOLUTION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 BRIGHAM & WOMENS HOSP,CTR OPHTHALM RES,BOSTON,MA 02115. NEI,LMDB,BETHESDA,MD 20892. RI Wawrousek, Eric/A-4547-2008 NR 0 TC 3 Z9 3 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S772 EP S772 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503556 ER PT J AU KOZHICH, AT CASPI, RR GERY, I AF KOZHICH, AT CASPI, RR GERY, I TI CONSTRUCTION OF SUPERIOR UVEITOGENIC EPITOPES AND THEIR PROPERTIES SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S858 EP S858 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503921 ER PT J AU KUTTY, RK KUTTY, G HOOKS, JJ CHADER, GJ WIGGERT, B NAGINENI, CN AF KUTTY, RK KUTTY, G HOOKS, JJ CHADER, GJ WIGGERT, B NAGINENI, CN TI INCREASED EXPRESSION OF THE INDUCIBLE FORM OF NITRIC-OXIDE SYNTHASE MESSENGER-RNA IN HUMAN RETINAL-PIGMENT EPITHELIAL (RPE) CELLS BY CYTOKINES AND ITS INHIBITION BY TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S204 EP S204 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500938 ER PT J AU LACKNER, PA SATO, S LIZAK, MJ WYMAN, M KADOR, PF AF LACKNER, PA SATO, S LIZAK, MJ WYMAN, M KADOR, PF TI AGE-DEPENDENT LENS CHANGES IN GALACTOSE-FED DOGS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OCULAR THERAPEUT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S799 EP S799 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503692 ER PT J AU LAI, JC WAWROUSEK, EF FUKUSHIMA, A LOBANOFF, MC LEE, RS WHITCUP, SM SMITHGILL, S GERY, I AF LAI, JC WAWROUSEK, EF FUKUSHIMA, A LOBANOFF, MC LEE, RS WHITCUP, SM SMITHGILL, S GERY, I TI IMMUNOTOLERANCE IN TRANSGENIC (TG) MICE EXPRESSING A FOREIGN ANTIGEN IN THEIR LENS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NCI,BETHESDA,MD 20892. NIH,HOWARD HUGHES MED INST,RES SCHOLARS PROBGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S201 EP S201 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500928 ER PT J AU LAVER, NM JACOT, JL GLOVER, JP LAZAROUS, DF UNGER, EF SHAH, SM ROBISON, WG AF LAVER, NM JACOT, JL GLOVER, JP LAZAROUS, DF UNGER, EF SHAH, SM ROBISON, WG TI ABSENCE OF RETINAL NEOVASCULARIZATION FOLLOWING CHRONIC, SYSTEMIC BASIC FIBROBLAST GROWTH-FACTOR ADMINISTRATION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 GEORGETOWN UNIV,SCH MED,WASHINGTON,DC 20057. NEI,BETHESDA,MD 20892. NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S402 EP S402 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91501865 ER PT J AU LEE, J JIAO, X CHADER, GJ AF LEE, J JIAO, X CHADER, GJ TI CULTURED MONKEY PIGMENT EPITHELIAL (PE) CELLS CONTAIN A FATTY-ACID-BINDING PROTEIN (FABP) THAT BINDS DOCOSAHEXAENOIC ACID (DHA) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S138 EP S138 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500670 ER PT J AU LI, X MA, W BARKER, JL PIATIGORSKY, J AF LI, X MA, W BARKER, JL PIATIGORSKY, J TI TRANSIENT EXPRESSION OF GLUTAMATE-DECARBOXYLASE (GAD67) IN THE DEVELOPING RAT LENS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,MOLEC & DEV BIOL LAB,BETHESDA,MD 20892. NINCDS,NEUROPHYSIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S176 EP S176 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500841 ER PT J AU LIZAK, MJ MORI, K CECKLER, TL BALABAN, RS KADOR, PF AF LIZAK, MJ MORI, K CECKLER, TL BALABAN, RS KADOR, PF TI QUANTITATION OF OSMOTIC CATARACTS IN GALACTOSE-FED BEAGLES USING MAGNETIZATION-TRANSFER CONTRAST-ENHANCED MAGNETIC-RESONANCE-IMAGING SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OCULAR THERAPEUT LAB,BETHESDA,MD 20892. NHLBI,CARDIAC ENERGET LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S801 EP S801 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503700 ER PT J AU LOBANOFF, MC LAI, JC WAWROUSEK, EF FUKUSHIMA, A LEE, RS CHAN, CC WHITCUP, SM GERY, I AF LOBANOFF, MC LAI, JC WAWROUSEK, EF FUKUSHIMA, A LEE, RS CHAN, CC WHITCUP, SM GERY, I TI CELL-MEDIATED, LENS-INDUCED UVEITIS IN TRANSGENIC (TG) MICE - A NOVEL EXPERIMENTAL EYE DISEASE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NIH,HOWARD HUGHES MED INST,RES SCHOLARS PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S202 EP S202 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500931 ER PT J AU LU, SC NAGINENI, CN HOOKS, JJ KANNAN, R AF LU, SC NAGINENI, CN HOOKS, JJ KANNAN, R TI BIDIRECTIONAL TRANSPORT OF INTACT GLUTATHIONE (GSH) BY CULTURED HUMAN RETINAL PIGMENTED EPITHELIAL-CELLS (HRPE) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 USC,SCH MED,DIV GASSTROINTESTINAL & LIVER DIS,LOS ANGELES,CA. NEI,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S520 EP S520 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502402 ER PT J AU LUYO, DA LI, Q PENG, B CHAN, CC AF LUYO, DA LI, Q PENG, B CHAN, CC TI TOPICAL CYCLOSPORINE INHIBITS MAST-CELL MEDIATED CONJUNCTIVITIS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S839 EP S839 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503853 ER PT J AU MAGNO, BV DATILES, MB LASA, MSM AF MAGNO, BV DATILES, MB LASA, MSM TI EVALUATION OF VISUAL FUNCTION FOLLOWING ND-YAG LASER POSTERIOR CAPSULOTOMY SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OPHTHALM GENET & CLIN SERV BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S812 EP S812 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503761 ER PT J AU MAHDI, RM SMITH, JA NUSSENBLATT, RB EGWUAGU, CE AF MAHDI, RM SMITH, JA NUSSENBLATT, RB EGWUAGU, CE TI CHARACTERIZATION OF RETINAL GAMMA/DELTA+ T-CELL REPERTOIRE DURING EXPERIMENTAL AUTOIMMUNE UVEORETINITIS (EAU) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S859 EP S859 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503926 ER PT J AU MAHURKAR, AA TRUS, BL VIVINO, MA KUEHL, EM DATILES, MB KAISERKUPFER, MI AF MAHURKAR, AA TRUS, BL VIVINO, MA KUEHL, EM DATILES, MB KAISERKUPFER, MI TI RETINAL FUNDUS PHOTO MONTAGES - A NEW COMPUTER-BASED METHOD SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OPHTHALM GENET & CLIN SERV BRANCH,BETHESDA,MD 20892. NIH,DIV COMP RES & TECHNOL,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 1 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S247 EP S247 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91501117 ER PT J AU MARTIN, DF PARKS, DJ MELLOW, SD FERRIS, FL WALTON, RC REMALEY, NA CHEW, EY ASHTON, P DAVIS, MD NUSSENBLATT, RB AF MARTIN, DF PARKS, DJ MELLOW, SD FERRIS, FL WALTON, RC REMALEY, NA CHEW, EY ASHTON, P DAVIS, MD NUSSENBLATT, RB TI TREATMENT OF CYTOMEGALOVIRUS RETINITIS WITH AN INTRAOCULAR SUSTAINED-RELEASE GANCICLOVIR IMPLANT - A RANDOMIZED CONTROLLED CLINICAL-TRIAL SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEW ENGLAND EYE CTR,BOSTON,MA. NEI,BETHESDA,MD 20892. EMORY UNIV,ATLANTA,GA 30322. UNIV WISCONSIN,MADISON,WI 53706. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S820 EP S820 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503788 ER PT J AU MILLERRIVERO, NE RIZZO, LV STIFF, LR CHAN, CC WIGGERT, B NUSSENBLATT, RB CASPI, RR AF MILLERRIVERO, NE RIZZO, LV STIFF, LR CHAN, CC WIGGERT, B NUSSENBLATT, RB CASPI, RR TI SUPPRESSION OF IRBP-INDUCED EU IN MICE DEFICIENT IN IL-4 AND IL-10 SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 MARYLAND GEN HOSP,BALTIMORE,MD. NEI,BETHESDA,MD 20892. RI Rizzo, Luiz Vicente/B-4458-2009 NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S201 EP S201 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500930 ER PT J AU MURPHY, CJ RUSSELL, P REID, TW AF MURPHY, CJ RUSSELL, P REID, TW TI SUBSTANCE-P ACCELERATES CORNEAL EPITHELIAL WOUND-HEALING OF GALACTOSEMIC RATS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV WISCONSIN,SCH VET MED,DEPT SURG SCI,MADISON,WI 53706. TEXAS TECH UNIV,LUBBOCK,TX 79409. NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S572 EP S572 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502652 ER PT J AU NAGINENI, C MARTINS, MC DETRICK, B HOOKS, JJ AF NAGINENI, C MARTINS, MC DETRICK, B HOOKS, JJ TI INTERFERON-GAMMA INHIBITS TOXOPLASMA-GONDII REPLICATION IN HUMAN RPE CELLS BY NITRIC-OXIDE - INDEPENDENT MECHANISMS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. GEORGE WASHINGTON UNIV,MED CTR,WASHINGTON,DC 20037. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S787 EP S787 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503630 ER PT J AU NEUENSCHWANDER, H TAKAHASHI, Y KADOR, PF AF NEUENSCHWANDER, H TAKAHASHI, Y KADOR, PF TI QUANTIFICATION OF RETINAL VESSEL CHANGES ASSOCIATED WITH DIABETIC-RETINOPATHY IN GALACTOSE-FED DOGS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OCULAR THERAPEUT LAB,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S485 EP S485 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502239 ER PT J AU NUSSENBLATT, RB WHITCUP, SM DESMET, MD CASPI, RR RIZZO, LV WEINER, H GERY, I AF NUSSENBLATT, RB WHITCUP, SM DESMET, MD CASPI, RR RIZZO, LV WEINER, H GERY, I TI THE USE OF ORAL TOLERANCE IN THE MANIPULATION OF THE IMMUNE-RESPONSE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,IMMUNOL LAB,BETHESDA,MD 20892. HARVARD UNIV,BRIGHAM & WOMENS HOSP,BOSTON,MA 02115. RI Rizzo, Luiz Vicente/B-4458-2009 NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S11 EP S11 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500043 ER PT J AU PALMON, FE JOPLIN, AC DVORAK, JA OBRITCH, WF CHAN, C MOYES, AL HOLLAND, EJ AF PALMON, FE JOPLIN, AC DVORAK, JA OBRITCH, WF CHAN, C MOYES, AL HOLLAND, EJ TI HUMAN EPITHELIAL-CELL VIABILITY FOLLOWING KERATOEPITHELIOPLASTY IN A RABBIT MODEL SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV MINNESOTA,DEPT OPHTHALMOL,MINNEAPOLIS,MN 55455. EYE CTR FLORIDA,FT MYERS,FL. NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S696 EP S696 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503190 ER PT J AU PENG, B LI, Q ROBERGE, FG NUSSENBLATT, RB CHAN, CC AF PENG, B LI, Q ROBERGE, FG NUSSENBLATT, RB CHAN, CC TI THE ROLE OF TRANSFORMING GROWTH-FACTOR-BETA-1 (TGF-BETA-1) IN ENDOTOXIN-INDUCED UVEITIS (EIU) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S545 EP S545 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502527 ER PT J AU PEREZ, J HAMASAKI, DI REDMOND, M SHINOHARA, T AF PEREZ, J HAMASAKI, DI REDMOND, M SHINOHARA, T TI MORPHOLOGICAL-CHANGES INDUCED BY INOCULATION OF AN ESCHERICHIA-COLI EXPRESSING A 65 KD RPE PROTEIN SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. B&WH,CTR OPHTHALMOL,BOSTON,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S763 EP S763 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503518 ER PT J AU PRENDERGAST, RA COSKUNCAN, NM LUTTY, GA MCLEOD, DS CASPI, RR AF PRENDERGAST, RA COSKUNCAN, NM LUTTY, GA MCLEOD, DS CASPI, RR TI ANTERIOR SEGMENT AND RETINAL INVOLVEMENT IN EAU DEPENDS UPON MATURATION OF ANTIGEN-PRESENTING CELLS (APC) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 JHU,APPL PHYS LAB,LAUREL,MD. NEI,BETHESDA,MD 20892. JOHNS HOPKINS UNIV HOSP,WILMER OPHTHALMOL INST,BALTIMORE,MD 21205. NR 1 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S859 EP S859 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503927 ER PT J AU QIN, C ROBISON, WG ZIGLER, JS AF QIN, C ROBISON, WG ZIGLER, JS TI HISTOLOGICAL ANALYSIS OF CATARACT DEVELOPMENT IN GUINEA-PIGS WITH MUTATION OF THE ZETA-CRYSTALLIN GENE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S799 EP S799 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503691 ER PT J AU RAO, PV TABOR, Y ZIGLER, JS GARLAND, D AF RAO, PV TABOR, Y ZIGLER, JS GARLAND, D TI 2-D ANALYSIS OF BLUE SEPHAROSE BOUND PROTEINS FROM THE LENS OF DIFFERENT SPECIES SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,MECHANISMS OCULAR DIS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S883 EP S883 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504042 ER PT J AU REDMOND, TM HARRIS, EW YU, S LIU, SY KAPSIS, A HAMEL, CP AF REDMOND, TM HARRIS, EW YU, S LIU, SY KAPSIS, A HAMEL, CP TI ANALYSIS OF THE HUMAN GENE FOR THE RETINAL-PIGMENT EPITHELIUM-SPECIFIC PROTEIN RPE65 SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. INSERM,U254,F-34100 MONTPELLIER,FRANCE. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S598 EP S598 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502770 ER PT J AU REMALEY, N CHEW, E SUGIMOTO, T PODGOR, M BATEMAN, B KLEBANOFF, M AF REMALEY, N CHEW, E SUGIMOTO, T PODGOR, M BATEMAN, B KLEBANOFF, M TI RISK-FACTORS FOR CONGENITAL CATARACTS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. UNIV CALIF LOS ANGELES,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S806 EP S806 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503730 ER PT J AU RENGARAJAN, K DESMET, MD KEDAR, I KOZHICH, AT CHADER, GJ WIGGERT, B AF RENGARAJAN, K DESMET, MD KEDAR, I KOZHICH, AT CHADER, GJ WIGGERT, B TI BINDING OF HEAT-SHOCK 70 PROTEINS FROM HUMAN AND RAB B-CELLS TO IRBP PEPTIDES AND PATHOGENICITY OF PEPTIDES IN THE LEWIS RAT SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S123 EP S123 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500601 ER PT J AU REUTER, LM CARUSO, RC LOPEZ, P KAISERKUPFER, MI AF REUTER, LM CARUSO, RC LOPEZ, P KAISERKUPFER, MI TI EFFECT OF PUPIL SIZE ON THE GANZFELD ELECTRORETINOGRAM SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OPHTHALM GENET & CLIN SERV BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S924 EP S924 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504238 ER PT J AU ROBISON, WG LAVER, NM JACOT, JL HOHMAN, TC AF ROBISON, WG LAVER, NM JACOT, JL HOHMAN, TC TI PREVENTION OF CATARACTS AND AMELIORATION OF DIABETIC-LIKE RETINOPATHY WITH THE ALDOSE REDUCTASE INHIBITOR WAY-121,509 SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. GEORGETOWN UNIV,SCH MED,WASHINGTON,DC 20007. WYETH AYERST LABS RES INC,PRINCETON,NJ. NR 0 TC 1 Z9 1 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S173 EP S173 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500829 ER PT J AU RUSSELL, P JOHNSON, DH AF RUSSELL, P JOHNSON, DH TI 2-DIMENSIONAL GEL-ELECTROPHORESIS OF SEGMENTS OF HUMAN TRABECULAR MESHWORK SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. MAYO CLIN & MAYO FDN,ROCHESTER,MN 55905. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S129 EP S129 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500624 ER PT J AU SARTANI, G SILVER, PB VELEZ, G CHAN, CC WIGGERT, B MASTORAKOS, G CASPI, RR AF SARTANI, G SILVER, PB VELEZ, G CHAN, CC WIGGERT, B MASTORAKOS, G CASPI, RR TI ANTI-TNF THERAPY CAN SUPPRESS THE INDUCTION OF EAU IN MICE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NICHHD,BETHESDA,MD. NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S859 EP S859 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503924 ER PT J AU SATO, S SECCHI, EF FUKASE, S LIZAK, MJ KADOR, PF AF SATO, S SECCHI, EF FUKASE, S LIZAK, MJ KADOR, PF TI POLYOL PATHWAY IN CULTURED DOG PERICYTES SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OCULAR THERAPEUT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S1067 EP S1067 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504899 ER PT J AU SECCHI, EF LIZAK, MJ SATO, S KADOR, PF AF SECCHI, EF LIZAK, MJ SATO, S KADOR, PF TI POLYOL PATHWAY FLUX MEASUREMENTS IN DOG LENS BY F-19-NMR SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,OCULAR THERAPEUT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S886 EP S886 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504056 ER PT J AU SHASTRY, BS HEJTMANCIK, JF PLAGER, DA HARTZER, MK TRESE, MT AF SHASTRY, BS HEJTMANCIK, JF PLAGER, DA HARTZER, MK TRESE, MT TI X-LINKED FAMILIAL EXUDATIVE VITREORETINOPATHY (FEVR) - LINKAGE ANALYSIS AND MUTATION WITHIN A CANDIDATE GENE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 OAKLAND UNIV,EYE RES INST,ROCHESTER,MI 48063. NEI,BETHESDA,MD 20892. INDIANA UNIV,BLOOMINGTON,IN 47401. WILLIAM BEAUMONT HOSP,ROYAL OAK,MI 48072. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S893 EP S893 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504094 ER PT J AU SI, Q PENG, B LUYO, D CHAN, CC AF SI, Q PENG, B LUYO, D CHAN, CC TI EXPRESSION OF PHOSPHOLIPASE A2S (PLA2S) IN MURINE ALLERGIC CONJUNCTIVITIS - KINETICS AND MODULATION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S1025 EP S1025 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504738 ER PT J AU SILVER, PB RIZZO, LV SARTANI, G CHAN, CC WIGGERT, B NUSSENBLATT, RB CASPI, RR AF SILVER, PB RIZZO, LV SARTANI, G CHAN, CC WIGGERT, B NUSSENBLATT, RB CASPI, RR TI HETEROLOGOUS EPITOPES OF IRBP PROTECT AGAINST AUTOIMMUNE UVEITIS INDUCED BY THE AUTOLOGOUS EPITOPE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. RI Rizzo, Luiz Vicente/B-4458-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S858 EP S858 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503922 ER PT J AU SMITH, JA WHITCUP, S MAHDI, RM NUSSENBLATT, RB EGWUAGU, CE AF SMITH, JA WHITCUP, S MAHDI, RM NUSSENBLATT, RB EGWUAGU, CE TI T-CELL RECEPTOR-GAMMA/DELTA USAGE IN HUMAN OCULAR SARCOIDOSIS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S537 EP S537 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502497 ER PT J AU SMITH, SB WONG, P BORA, N KUTTY, G MCCOOL, D KUTTY, K CHADER, G WIGGERT, B AF SMITH, SB WONG, P BORA, N KUTTY, G MCCOOL, D KUTTY, K CHADER, G WIGGERT, B TI PHOTORECEPTOR CELLS DIE BY APOPTOSIS IN THE VITILIGO M(VIT)/MI(VIT) MOUSE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 MED COLL GEORGIA,DEPT CELL BIOL & ANAT,AUGUSTA,GA 30912. MED COLL GEORGIA,DEPT OPHTHALMOL,AUGUSTA,GA 30912. NEI,LRCMB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S638 EP S638 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502912 ER PT J AU STIFF, LR RIZZO, LV CHOI, R CASPI, RR AF STIFF, LR RIZZO, LV CHOI, R CASPI, RR TI THE ROLE OF TH1 AND TH2 TYPE CYTOKINES IN THE DEVELOPMENT AND TREATMENT OF EAU SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 HOWARD HUGHES MED INST NIH,RES SCHOLARS PROGRAM,BETHESDA,MD. NEI,BETHESDA,MD 20892. RI Rizzo, Luiz Vicente/B-4458-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S859 EP S859 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503925 ER PT J AU SULLIVAN, D ANGLADE, E SALAMON, C HOOKS, J NUSSENBLATT, R CSAKY, K AF SULLIVAN, D ANGLADE, E SALAMON, C HOOKS, J NUSSENBLATT, R CSAKY, K TI ADENOVIRUS-MEDIATED GENE-TRANSFER OF TRANSFORMING GROWTH-FACTOR-BETA-1 AND ORNITHINE AMINOTRANSFERASE IN CULTURED HUMAN BPE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S98 EP S98 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500473 ER PT J AU SUN, B SILVER, PB WELLS, J WILDER, RL CASPI, RR AF SUN, B SILVER, PB WELLS, J WILDER, RL CASPI, RR TI ANALYSIS OF CYTOKINES PRODUCED BY SUSCEPTIBLE AND RESISTANT RATS IMMUNIZED WITH A PATHOGENIC PEPTIDE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NIAMS,BETHESDA,MD. NEI,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S858 EP S858 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503923 ER PT J AU SUZUKI, S LI, Q PENG, B WIGGERT, BN KOHN, LD NUSSENBLATT, RB CHAN, CC AF SUZUKI, S LI, Q PENG, B WIGGERT, BN KOHN, LD NUSSENBLATT, RB CHAN, CC TI METHIMAZOLE (MMI), AN AGENT CAPABLE OF REDUCING MHC CLASS-I EXPRESSION, INHIBITS EXPERIMENTAL MELANIN-INDUCED UVEITIS (EMIU) AND EXPERIMENTAL AUTOIMMUNE UVEORETINITIS (EAU) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NIDDKD,BETHESDA,MD. NR 1 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S542 EP S542 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502512 ER PT J AU TILLER, GE HEINZMANN, C KOJIS, TL GONZALEZ, P BATEMAN, JB AF TILLER, GE HEINZMANN, C KOJIS, TL GONZALEZ, P BATEMAN, JB TI LINKAGE MAPPING OF ZETA-CRYSTALLIN ON HUMAN CHROMOSOME-1P - APPLICATION TO HEREDITARY CATARACTS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, JULES STEIN EYE INST, LOS ANGELES, CA USA. VANDERBILT UNIV, MED CTR, NASHVILLE, TN 37240 USA. NIH, BETHESDA, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S879 EP S879 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504018 ER PT J AU TOHAH, H LE, D CHAN, CC NUSSENBLATT, RB AF TOHAH, H LE, D CHAN, CC NUSSENBLATT, RB TI THE IN-VITRO EFFECTS OF CYCLOSPORINE-A (CSA) ON CORNEA SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV ULSAN,DEPT OPHTHALMOL,SEOUL,SOUTH KOREA. NEI,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S300 EP S300 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91501389 ER PT J AU TOMAREV, SI ZINOVIEVA, RD DUNCAN, MK BANERJEEBASU, S JOHNSON, T SUNDIN, O YANG, JM PIATIGORSKY, J AF TOMAREV, SI ZINOVIEVA, RD DUNCAN, MK BANERJEEBASU, S JOHNSON, T SUNDIN, O YANG, JM PIATIGORSKY, J TI HOMEOBOX GENE PROX-1 AND LENS DEVELOPMENT SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,SCH MED,WILMER EYE INST,BALTIMORE,MD 21205. NEI,MOLEC & DEV BIOL LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S844 EP S844 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503871 ER PT J AU TOYAMA, ES OKAMOTO, S MAKONNEN, S SUNDARRAJ, N HASSELL, JR SATO, S KADOR, PF AF TOYAMA, ES OKAMOTO, S MAKONNEN, S SUNDARRAJ, N HASSELL, JR SATO, S KADOR, PF TI DEVELOPMENT OF AN IN-VITRO MODEL OF GALACTOSE-INDUCED KERATOEPITHELIOPATHY USING A CORNEAL EPITHELIAL-CELL LINE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV PITTSBURGH,DEPT OPHTHALMOL,PITTSBURGH,PA 15260. NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S701 EP S701 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503218 ER PT J AU TUMMINIA, SJ RUSSELL, P AF TUMMINIA, SJ RUSSELL, P TI CATARACT FORMATION IN TRANSGENIC MICE CONTAINING HIV-1 PROTEASE LINKED TO THE ALPHA-A-CRYSTALLIN PROMOTER SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,MECHANISMS OCULAR DIS LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S879 EP S879 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504015 ER PT J AU VISTICA, BP CHANAUD, NP FELIX, N RIZZO, LV SILVER, PB CASPI, RR NUSSENBLATT, RB GERY, T AF VISTICA, BP CHANAUD, NP FELIX, N RIZZO, LV SILVER, PB CASPI, RR NUSSENBLATT, RB GERY, T TI CD8 T-CELLS ARE NOT ESSENTIAL FOR THE INDUCTION OF ORAL TOLERANCE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. AUTOIMMUNE INC,LEXINGTON,MA. RI Rizzo, Luiz Vicente/B-4458-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S543 EP S543 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502518 ER PT J AU WALTON, RC LUYO, DA NUSSENBLATT, RB CHAN, CC AF WALTON, RC LUYO, DA NUSSENBLATT, RB CHAN, CC TI INTERLEUKIN-10 EXPRESSION IN PATIENTS WITH UVEITIS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S537 EP S537 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502492 ER PT J AU WANG, X OHTAKAMARUYAMA, C CHEPELINSKY, AB AF WANG, X OHTAKAMARUYAMA, C CHEPELINSKY, AB TI TRANSCRIPTIONAL REGULATION OF THE HUMAN MIP GENE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,MOLEC & DEV BIOL LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S882 EP S882 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504034 ER PT J AU WANG, Y COHEN, JI PERERA, L STRAUS, S NUSSENBLATT, R HOOKS, JJ AF WANG, Y COHEN, JI PERERA, L STRAUS, S NUSSENBLATT, R HOOKS, JJ TI EXPERIMENTAL CHRONIC UVEITIS IN GUINEA-PIGS INFECTED WITH HUMAN VARICELLA-ZOSTER VIRUS (VZV) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S150 EP S150 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500729 ER PT J AU WAWROUSEK, EF BRADY, JP AF WAWROUSEK, EF BRADY, JP TI IN-VIVO INVESTIGATION OF CRYSTALLIN FUNCTION USING ALPHA-CRYSTALLIN GENE KNOCKOUT MICE SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S882 EP S882 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91504035 ER PT J AU WHITCUP, SM LOBANOFF, M GERY, I ISHIMOTO, S WOLITSKY, B NUSSENBLATT, RB CHAN, CC AF WHITCUP, SM LOBANOFF, M GERY, I ISHIMOTO, S WOLITSKY, B NUSSENBLATT, RB CHAN, CC TI ROLE OF SELECTINS AND INTEGRINS IN THE PATHOGENESIS OF ENDOTOXIN-INDUCED UVEITIS (EIU) SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. HOFFMANN LA ROCHE INC,NUTLEY,NJ 07110. NR 0 TC 3 Z9 3 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S385 EP S385 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91501805 ER PT J AU WONG, P ULYANOVA, T DARROW, R SHIVARAM, S VANVEEN, T CHADER, G ORGANISCIAK, D AF WONG, P ULYANOVA, T DARROW, R SHIVARAM, S VANVEEN, T CHADER, G ORGANISCIAK, D TI CORRELATION OF LIGHT-INDUCED RETINAL DAMAGE IN RATS WITH CHANGES IN TRPM-2/CLUSTERIN (TRPM-2) EXPRESSION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. GOTHENBURG UNIV,DEPT ZOOL,S-41124 GOTHENBURG,SWEDEN. WRIGHT STATE UNIV,DEPT BIOCHEM & MOLEC BIOL,DAYTON,OH 45435. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S860 EP S860 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503931 ER PT J AU WU, YQ BECERRA, SP AF WU, YQ BECERRA, SP TI PEDF PROCESSING IN IPM AND VITREOUS SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,RETINAL CELL & MOLEC BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S131 EP S131 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500635 ER PT J AU XU, H REDMOND, M WAWROUSEK, EF NICKERSON, JM CASPI, RR AF XU, H REDMOND, M WAWROUSEK, EF NICKERSON, JM CASPI, RR TI STUDIES OF TOLERANCE DEVELOPMENT TO RETINAL ANTIGENS USING TRANSGENIC TECHNOLOGY .1. CONSTRUCTION OF THE GENES OF INTEREST SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. EMORY UNIV,DEPT OPHTHALMOL,ATLANTA,GA 30322. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S540 EP S540 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91502504 ER PT J AU YU, S LIU, SY DETRICK, B HOOKS, JJ REDMOND, TM AF YU, S LIU, SY DETRICK, B HOOKS, JJ REDMOND, TM TI OVER-EXPRESSION AND PURIFICATION OF RPE65 PROTEIN SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. GEORGE WASHINGTON UNIV,WASHINGTON,DC 20052. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S763 EP S763 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91503519 ER PT J AU ZADUNAISKY, JA SPRING, KR AF ZADUNAISKY, JA SPRING, KR TI TBM CELLS AREA CHANGES INDUCED BY DRUGS - IS IT CONTRACTION OR CELL-VOLUME REGULATION SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NYU MED CTR,NEW YORK,NY 10016. NIH,BETHESDA,MD 20892. NR 0 TC 6 Z9 6 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S194 EP S194 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500905 ER PT J AU ZELENKA, PS ARORA, JK LYSZ, TW AF ZELENKA, PS ARORA, JK LYSZ, TW TI A ROLE FOR 12(S)HETE IN THE RESPONSE OF HUMAN LENS EPITHELIAL-CELLS TO EGF AND INSULIN SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,NEWARK,NJ 07103. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1995 VL 36 IS 4 BP S176 EP S176 PG 1 WC Ophthalmology SC Ophthalmology GA QM915 UT WOS:A1995QM91500842 ER PT J AU ANDERSON, EL DECKER, MD ENGLUND, JA EDWARDS, KM ANDERSON, P MCINNES, P BELSHE, RB AF ANDERSON, EL DECKER, MD ENGLUND, JA EDWARDS, KM ANDERSON, P MCINNES, P BELSHE, RB TI INTERCHANGEABILITY OF CONJUGATED HAEMOPHILUS-INFLUENZAE TYPE-B VACCINES IN INFANTS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID OUTER-MEMBRANE PROTEIN; 6-MONTH-OLD INFANTS; POLYSACCHARIDE; IMMUNOGENICITY; DISEASE; 2-MONTH-OLD; CHILDREN; TRIAL AB Objective.-To evaluate the safety and immunogenicity of two Haemophilus influenzae type b (Hib) conjugate vaccines when administered in serial combination, These vaccines consisted of Hib capsular polysaccharide polyribosyl-ribitol phosphate (PRP) conjugated to the meningococcal outer membrane protein (OMP) complex (PRP-OMP) and H influenzae oligosaccharide conjugated to a mutant toxin (CRM197) isolated from Corynebacterium diphtheriae (HbOC). Design.-Randomized, double-blind, clinical trial evaluating five Hib vaccination regimens. Setting.-Vaccine Treatment and Evaluation Units and affiliated private pediatric practices at Saint Louis (Mo) University, Vanderbilt University, Nashville, Tenn, and Baylor College of Medicine, Houston, Tex. Patients.-A total of 497 healthy 2-month-old infants scheduled to receive routine immunization. Intervention.-Participants received either PRP-OMP or HbOC given as recommended by the manufacturer, PRP-OMP at 2 and 6 months, HbOC at 2 months, then PRP-OMP at 4 and 6 months, or PRP-OMP at 2 months and then HbOC at 4 and 6 months. Unconjugated PRP was given at 15 months to evaluate priming. Results.-Geometric mean antibody concentrations differed significantly among the groups following the second and third immunizations of the primary series and following booster immunization with unconjugated PRP. On each occasion, the groups receiving serial combinations of PRP-OMP and HbOC achieved mean antibody concentrations that equalled or exceeded those of the groups receiving a single product. Adverse reactions did not vary by group. Conclusions.-The studied sequential combinations of Hib vaccines were safe and at least as immunogenic as either vaccine alone. C1 ST LOUIS UNIV,SCH MED,DEPT PEDIAT,ST LOUIS,MO 63110. VANDERBILT UNIV,SCH MED,DEPT PREVENT MED,NASHVILLE,TN 37212. VANDERBILT UNIV,SCH MED,DEPT MED,NASHVILLE,TN 37212. VANDERBILT UNIV,SCH MED,DEPT PEDIAT,NASHVILLE,TN 37212. BAYLOR COLL MED,DEPT MICROBIOL & IMMUNOL,HOUSTON,TX 77030. UNIV ROCHESTER,SCH MED & DENT,DEPT PEDIAT,ROCHESTER,NY 14642. NIAID,DIV MICROBIOL & INFECT DIS,RESP DIS BRANCH,BETHESDA,MD 20892. RP ANDERSON, EL (reprint author), ST LOUIS UNIV,HLTH SCI CTR,SCH MED,DEPT MED,DIV INFECT DIS,3635 VISTA AVE,FDT-8N,POB 15250,ST LOUIS,MO 63110, USA. FU NIAID NIH HHS [N01-AI-02645, N01-AI-05051, N01-AI-72629] NR 20 TC 27 Z9 29 U1 0 U2 2 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD MAR 15 PY 1995 VL 273 IS 11 BP 849 EP 853 DI 10.1001/jama.273.11.849 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA QL402 UT WOS:A1995QL40200021 PM 7869554 ER PT J AU CANNON, RO AF CANNON, RO TI THE SENSITIVE HEART - A SYNDROME OF ABNORMAL CARDIAC PAIN PERCEPTION SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Discussion ID NORMAL CORONARY ARTERIOGRAMS; LEFT-VENTRICULAR HYPERTROPHY; ESOPHAGEAL CHEST PAIN; SYNDROME-X; ANGINA-PECTORIS; FOLLOW-UP; FLOW RESERVE; ENDOTHELIAL DYSFUNCTION; RESISTANCE VESSELS; ARTERY DISEASE RP CANNON, RO (reprint author), NHLBI,CARDIOL BRANCH,BLDG 10,ROOM 7B-15,BETHESDA,MD 20892, USA. NR 62 TC 39 Z9 41 U1 1 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD MAR 15 PY 1995 VL 273 IS 11 BP 883 EP 887 DI 10.1001/jama.273.11.883 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA QL402 UT WOS:A1995QL40200028 PM 7869561 ER PT J AU PRATOLONGO, R PERICO, A FREED, KF SZABO, A AF PRATOLONGO, R PERICO, A FREED, KF SZABO, A TI MULTIEXPONENTIAL APPROXIMATIONS TO THE TORSIONAL TIME-CORRELATION FUNCTION FOR ONE-DIMENSIONAL SYSTEMS WITH MANY BARRIERS SO JOURNAL OF CHEMICAL PHYSICS LA English DT Article ID CONFORMATIONAL TRANSITIONS; RELAXATION; DYNAMICS; POLYMERS; MACROMOLECULES; DIFFUSION; KINETICS C1 UNIV CHICAGO,JAMES FRANCK INST,CHICAGO,IL 60637. UNIV CHICAGO,DEPT CHEM,CHICAGO,IL 60637. NIDDKD,PHYS CHEM LAB,BETHESDA,MD 20892. RP PRATOLONGO, R (reprint author), NATL RES COUNCIL,IST STUDI CHIMICOFIS MACROMOLEC SINTETICHE & NAT,GENOA,ITALY. RI Szabo, Attila/H-3867-2012 NR 15 TC 7 Z9 7 U1 0 U2 1 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0021-9606 J9 J CHEM PHYS JI J. Chem. Phys. PD MAR 15 PY 1995 VL 102 IS 11 BP 4683 EP 4690 DI 10.1063/1.469516 PG 8 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA QL735 UT WOS:A1995QL73500038 ER PT J AU ROMANOSPICA, V GEORGIOU, P SUZUKI, H PAPAS, TS BHAT, NK AF ROMANOSPICA, V GEORGIOU, P SUZUKI, H PAPAS, TS BHAT, NK TI ROLE OF ETS1 IN IL-2 GENE-EXPRESSION SO JOURNAL OF IMMUNOLOGY LA English DT Article ID LONG TERMINAL REPEAT; T-CELLS; INTERLEUKIN-2 GENE; ANTIGEN RECEPTOR; DNA-BINDING; C-ETS; TRANSCRIPTION FACTORS; PROTO-ONCOGENE; LYMPHOCYTES-T; ACTIVATION AB The ETS1 gene encodes a sequence-specific transcription factor binding to purine-rich DNA sequences (-GGAA-) present in the transcriptional regulatory regions of many cellular and viral promoters/enhancers, including many lymphokine genes. The ETS1 gene is expressed at high levels in resting T cells and at very low levels after T cell activation, suggesting it may suppress the expression of genes induced during T cell activation. To find out if ETS1 regulates expression of the IL-2 gene, we have ectopically expressed antisense (AS) ETS1 in jurkat T cells to block the formation of ETS1 proteins. AS ETS1 transfectants produce higher levels of IL-2 compared with sense ETS1 transfectants. Expression of ETS1 DNA binding domain in Jurkat T cells also decreased the production of IL-2. In AS ETS1 transfectants, IL-2 formation was completely inhibited by cyclosporin A and FK590. The IL-2 promoter linked to a chloramphenicol acetyl transferase reporter gene has high activity in AS ETS1 transfectants, indicating that increased IL-2 production seems to be a result of transcriptional induction. Taken together, these results suggest the possibility that ETS1 may act as a negative regulator of IL-2 gene transcription and provide a rational approach toward engineering the endogenous expression of IL-2 in T cells. C1 NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC DYNCORP,FREDERICK,MD 21702. NCI,MOLEC ONCOL LAB,FREDERICK,MD 21702. MED UNIV S CAROLINA,HOLLINGS CANC CTR,CTR MOLEC & STRUCT BIOL,CHARLESTON,SC 29425. NR 48 TC 35 Z9 35 U1 1 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 15 PY 1995 VL 154 IS 6 BP 2724 EP 2732 PG 9 WC Immunology SC Immunology GA QL084 UT WOS:A1995QL08400019 PM 7876544 ER PT J AU SHIRAI, M ARICHI, T NISHIOKA, M NOMURA, T IKEDA, K KAWANISHI, K ENGELHARD, VH FEINSTONE, SM BERZOFSKY, JA AF SHIRAI, M ARICHI, T NISHIOKA, M NOMURA, T IKEDA, K KAWANISHI, K ENGELHARD, VH FEINSTONE, SM BERZOFSKY, JA TI CTL RESPONSES OF HLA-A2.1-TRANSGENIC MICE SPECIFIC FOR HEPATITIS-C VIRAL PEPTIDES PREDICT EPITOPES FOR CTL OF HUMANS CARRYING HLA-A2.1 SO JOURNAL OF IMMUNOLOGY LA English DT Article ID NON-B-HEPATITIS; CYTOTOXIC LYMPHOCYTES-T; MAJOR HISTOCOMPATIBILITY COMPLEX; NON-A; CELL RECOGNITION; TRANSGENIC MICE; ANTIGEN PRESENTATION; MONOCLONAL-ANTIBODY; SYNTHETIC PEPTIDES; VIRUS-REPLICATION AB Vaccine development in animal models depends on ability to recognize epitopes seen by human T cells. In this work, we show that CTL responses in transgenic mice expressing human HLA-A2.1 prospectively predict the same four of 11 hepatitis C virus (HCV) structural protein-derived peptides, expressing a sequence motif for HLA-A2.1 binding, that are actually recognized by human A2.1-restricted CTLs. The CTLs also recognized targets endogenously expressing these proteins. Human CTLs from HCV-infected patients, tested by using the same peptides, revealed a virtually identical response repertoire. A highly conserved HCV core peptide was the most immunogenic, and may be a valuable component of a vaccine against a broad range of HCV isolates in HLA-A2-positive patients. These results suggest that, in spite of species differences, the T cell repertoire is plastic enough to allow a similar response when the same class I MHC molecule is presenting the peptide. Thus, the HLA molecule plays the primary role in determining which peptides are recognized by CTLs. This transgenic mouse model is important for the study of HLA-restricted CTL determinants and for an approach to design a potential HCV vaccine. C1 NCI,METAB BRANCH,MOLEC IMMUNOGENET & VACCINE RES SECT,BETHESDA,MD 20892. KAGAWA MED SCH,DEPT INTERNAL MED 3,KAGAWA 76107,JAPAN. KAGAWA MED SCH,DEPT TRANSFUS MED,KAGAWA 76107,JAPAN. UNIV VIRGINIA,DEPT MICROBIOL,CHARLOTTESVILLE,VA 22908. US FDA,CTR BIOL EVALUAT & RES,DIV VIROL,HEPATITIS RES LAB,BETHESDA,MD 20892. RI Yang, Chen/G-1379-2010 FU NIAID NIH HHS [R01 AI21393] NR 68 TC 151 Z9 154 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 15 PY 1995 VL 154 IS 6 BP 2733 EP 2742 PG 10 WC Immunology SC Immunology GA QL084 UT WOS:A1995QL08400020 PM 7533182 ER PT J AU KRUMHOLZ, HM LARSON, M LEVY, D AF KRUMHOLZ, HM LARSON, M LEVY, D TI PROGNOSIS OF LEFT-VENTRICULAR GEOMETRIC PATTERNS IN THE FRAMINGHAM HEART-STUDY SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID ESSENTIAL-HYPERTENSION; SEX-DIFFERENCES; MASS; HYPERTROPHY AB Objectives. The goal of this study was to determine the incremental mental prognostic value of left ventricular geometric patterns beyond that provided by cardiovascular disease risk factors, including left ventricular mass. Background. Left ventricular geometry may be classified into the following four mutually exclusive groups on the basis of left ventricular mass and relative wall thickness: concentric hypertrophy (increased mass and increased relative wall thickness), eccentric hypertrophy (increased mass and normal relative wall thickness), concentric remodeling (normal mass and increased relative wall thickness) and normal geometry (normal mass and normal relative wall thickness). The prognosis associated with these patterns in a population-based sample is not known. Methods. Proportional hazards regression models were used to evaluate the prognostic importance of left ventricular geometry in 3,216 subjects in the Framingham Heart Study who were greater than or equal to 40 years old and free of clinically apparent cardiovascular disease, after adjustment for traditional cardiovascular risk factors and left ventricular mass. The follow-up period was 8 years. Results. Subjects with concentric hypertrophy had the worst prognosis, followed by those with eccentric hypertrophy, concentric remodeling and normal geometry. Subjects with concentric hypertrophy also had the highest left ventricular mass. The association between type of geometry and prognosis was largely attenuated by adjustment for baseline differences in left ventricular mass, The odds ratio for incident cardiovascular disease in subjects with concentric hypertrophy compared with those who had normal geometry was 13 (95% confidence interval [CI] 0.8 to 2.1) in men and 1.2 (95% CI 0.6 to 2.3) in women after adjustment for other cardiovascular risk factors, including left ventricular mass. Conclusions. In a population-based sample of subjects without cardiovascular disease, knowledge of left ventricular geometry provided little prognostic information beyond that available from left ventricular mass and traditional cardiovascular risk factors. C1 FRAMINGHAM HEART DIS EPIDEMIOL STUDY,FRAMINGHAM,MA 01701. YALE UNIV,SCH MED,CARDIOVASC MED SECT,NEW HAVEN,CT. NHLBI,BETHESDA,MD 20892. BETH ISRAEL HOSP,DIV CARDIOVASC,BOSTON,MA 02215. BETH ISRAEL HOSP,DIV CLIN EPIDEMIOL,BOSTON,MA 02215. BOSTON UNIV,SCH MED,DIV EPIDEMIOL & PREVENT MED,BOSTON,MA 02118. NR 25 TC 335 Z9 351 U1 0 U2 3 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD MAR 15 PY 1995 VL 25 IS 4 BP 879 EP 884 DI 10.1016/0735-1097(94)00473-4 PG 6 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QL404 UT WOS:A1995QL40400011 PM 7884091 ER PT J AU BROWN, ML FIREMAN, B AF BROWN, ML FIREMAN, B TI EVALUATION OF DIRECT MEDICAL COSTS RELATED TO CANCER SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Editorial Material ID BREAST RP BROWN, ML (reprint author), NCI,DIV CANC PREVENT & CONTROL,SURVEILLANCE PROGRAM,APPL RES BRANCH,EXECUT PLAZA N,RM 313,BETHESDA,MD 20892, USA. NR 7 TC 10 Z9 10 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD MAR 15 PY 1995 VL 87 IS 6 BP 399 EP 400 DI 10.1093/jnci/87.6.399 PG 2 WC Oncology SC Oncology GA QL082 UT WOS:A1995QL08200001 PM 7861454 ER PT J AU HEINO, P EKLUND, C FREDERIKSSONSHANAZARIAN, V GOLDMAN, S SCHILLER, JT DILLNER, J AF HEINO, P EKLUND, C FREDERIKSSONSHANAZARIAN, V GOLDMAN, S SCHILLER, JT DILLNER, J TI ASSOCIATION OF SERUM IMMUNOGLOBULIN-G ANTIBODIES AGAINST HUMAN PAPILLOMAVIRUS TYPE-16 CAPSIDS WITH ANAL EPIDERMOID CARCINOMA SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID INVASIVE CERVICAL-CANCER; PROTEINS; L1 AB Background: Anal epidermoid carcinoma is a relatively rare tumor, but its incidence has been increasing rapidly during the past few years, Genetic material from the major oncogenic types of human papillomavirus (HPV), types 16 and 18, has regularly been demonstrated in a substantial proportion of anal cancers, suggesting an etiologic role of HPV infection, Recently, serum antibodies against HPV type 16 capsids were shown to be a serologic measure of HPV16 infection. Purpose: We investigated whether serum antibodies against HPV16 capsids are associated with an increased risk of developing anal cancer, Methods: Serum samples from 64 patients (48 women and 16 men) with untreated anal epidermoid cancer and from 79 age- and sex-matched healthy blood donors were analyzed for the levels of serum immunoglobulin G (IgG) against capsids of HPV16 by the enzyme-linked immunosorbent assay, The levels of serum IgG against HPV type 6 and bovine papillomavirus (BPV) capsids, as well as against HPV16 peptide antigens, were also measured, Results: Whereas antibodies against HPV6 or BPV capsids were not significantly associated with anal cancer, the presence of IgG against HPV16 capsids exceeding the anti-BPV antibody levels was demonstrated among 55% (35 of 64) of the case patients but only among 4% (three of 79) of the control subjects (odds ratio [OR] 30.4; 95% confidence interval [CI] = 8.4-161.5), Antibodies against HPV16 E2 and E7 peptides were also more common among case patients (OR = 12.8 and 95% CI = 5.4-31.5 for E2; OR = 3.0 and 95% CI = 1.4-6.7 for E7), Conclusion: The results suggest that HPV16 capsid antibodies are serologic markers for anal cancer, Implication: Exposure to HPV16 or related viruses appears to be a major risk factor in the majority of anal cancers. C1 KAROLINSKA INST,CTR MICROBIOL & TUMOR BIOL,S-17177 STOCKHOLM,SWEDEN. SODERSJUKHUSET HOSP,DEPT SURG,STOCKHOLM,SWEDEN. NCI,DIV CANC BIOL DIAG & CTR,CELLULAR ONCOL LAB,BETHESDA,MD 20892. NR 18 TC 57 Z9 57 U1 0 U2 1 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD MAR 15 PY 1995 VL 87 IS 6 BP 437 EP 440 DI 10.1093/jnci/87.6.437 PG 4 WC Oncology SC Oncology GA QL082 UT WOS:A1995QL08200011 PM 7532227 ER PT J AU QIN, J CLORE, GM KENNEDY, WM HUTH, JR GRONENBORN, AM AF QIN, J CLORE, GM KENNEDY, WM HUTH, JR GRONENBORN, AM TI SOLUTION STRUCTURE OF HUMAN THIOREDOXIN IN A MIXED DISULFIDE INTERMEDIATE COMPLEX WITH ITS TARGET PEPTIDE FROM THE TRANSCRIPTION FACTOR NF-KAPPA-B SO STRUCTURE LA English DT Article DE DISULFIDE-BONDED INTERMEDIATE; HUMAN THIOREDOXIN; TRANSCRIPTION FACTOR NF-KAPPA-B ID NUCLEAR MAGNETIC-RESONANCE; DNA-BINDING ACTIVITY; HETERONUCLEAR NMR-SPECTROSCOPY; RESTRAINED MOLECULAR-DYNAMICS; T-CELL LINES; DISTANCE GEOMETRY; 3-DIMENSIONAL STRUCTURE; RECEPTOR EXPRESSION; PROTEIN-STRUCTURE; ESCHERICHIA-COLI AB Background: Human thioredoxin is a 12 kDa cellular redox protein that plays a key role in maintaining the redox environment of the cell. It has recently been shown to be responsible for activating the DNA-binding properties of the cellular transcription factor, NF kappa B, by reducing a disulfide bond involving Cys62 of the p50 subunit. Using multidimensional heteronuclear-edited and heteronuclear-filtered NMR spectroscopy, we have solved the solution structure of a complex of human thioredoxin and a 13-residue peptide extending from residues 56-68 of p50, representing a kinetically stable mixed disulfide intermediate along the reaction pathway. Results: The NF kappa B peptide is located in a long boot-shaped cleft on the surface of human thioredoxin delineated by the active-site loop, helices alpha 2, alpha 3 and alpha 4, and strands beta 3 and beta 4. The peptide adopts a crescent-like conformation with a smooth 110 degrees bend centered around residue 60 which permits it to follow the path of the cleft. Conclusions: In addition to the intermolecular disulfide bridge between Cys32 of human thioredoxin and Cys62 of the peptide, the complex is stabilized by numerous hydrogen-bonding, electrostatic and hydrophobic interactions which involve residues 57-65 of the NF kappa B peptide and confer substrate specificity. These structural features permit one to suggest the specificity requirements for human thioredoxin-catalyzed disulfide bond reduction of proteins. C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 85 TC 192 Z9 195 U1 1 U2 2 PU CURRENT BIOLOGY LTD PI LONDON PA 34-42 CLEVELAND STREET, LONDON, ENGLAND W1P 6LB SN 0969-2126 J9 STRUCTURE JI Structure PD MAR 15 PY 1995 VL 3 IS 3 BP 289 EP 297 DI 10.1016/S0969-2126(01)00159-9 PG 9 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA QP156 UT WOS:A1995QP15600008 PM 7788295 ER PT J AU GAO, WS CONNOR, HD LEMASTERS, JJ MASON, RP THURMAN, RG AF GAO, WS CONNOR, HD LEMASTERS, JJ MASON, RP THURMAN, RG TI PRIMARY NONFUNCTION OF FATTY LIVERS PRODUCED BY ALCOHOL IS ASSOCIATED WITH A NEW, ANTIOXIDANT-INSENSITIVE FREE-RADICAL SPECIES SO TRANSPLANTATION LA English DT Article ID RAT-LIVER; TRANSPLANTATION; INJURY; REPERFUSION; STORAGE AB The formation of free radicals after orthotopic liver transplantation in the rat correlates with graft failure. Fatty livers from alcoholics transplant poorly, so these studies were designed to examine the effect of alcohol on free radical formation in a rearterialized rat liver transplantation model. Treatment of rats for 3-5 weeks with either a high-fat or an ethanol-containing liquid diet caused characteristic pericentral lipid accumulation. After storage in University of Wisconsin cold storage solution (UW) and transplantation, a reperfusion injury characterized by increased postoperative AST levels (greater than 1500 U/l in about 3 hours) was observed in rats fed high-fat or alcohol-containing diets, whereas parenchymal cell injury was seen much less in low-fat controls. Survival was around 63% in the low-fat group but decreased to 12 and 18% in the high-fat and alcohol groups, respectively. Furthermore, intracellular lipid content correlated inversely with survival. In untransplanted livers, the spin trap alpha-phenyl N-tert-butylnitrone (PBN) was infused, and blood samples were collected and extracted with chloroform:methanol. Signals indicative of carbon-centered PBN radical adducts were barely detectable in all untransplanted groups studied by electron paramagnetic resonance. In contrast, a robust 6-line complex spectrum was obtained from all groups studied immediately after 48 hours of cold storage in UW solution and transplantation. A mixture of 3 radical species was identified. Two had coupling constants similar to lipid-derived free radicals, whereas the third is a new species with unique coupling constants and is most likely oxygen derived. In low-fat controls, the signal was reduced significantly by superoxide dismutase (SOD)/catalase; however, SOD/catalase had no effect on free radicals in lipid-loaded livers. Thus, both dietary high fat and alcohol exposure produce a unique SOD/catalase-insensitive free radical species that may be involved in the mechanism of failure of fatty livers after orthotopic liver transplantation. C1 UNIV N CAROLINA,FLOB,DEPT PHARMACOL,HEPATOBIOL & TOXICOL LAB,CHAPEL HILL,NC 27599. KENTUCKY WESLEYAN COLL,DEPT CHEM,OWENSBORO,KY 42301. UNIV N CAROLINA,CURRICULUM TOXICOL,CHAPEL HILL,NC 27599. UNIV N CAROLINA,DEPT CELL BIOL & ANAT,CHAPEL HILL,NC 27599. NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709. FU NIAAA NIH HHS [AA-09156]; NIEHS NIH HHS [5T32ESO7126] NR 15 TC 54 Z9 55 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD MAR 15 PY 1995 VL 59 IS 5 BP 674 EP 679 DI 10.1097/00007890-199503150-00005 PG 6 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA QM656 UT WOS:A1995QM65600005 PM 7886790 ER PT J AU MAHONEY, CW HUANG, KP AF MAHONEY, CW HUANG, KP TI SELECTIVE PHOSPHORYLATION OF CATIONIC POLYPEPTIDE AGGREGATED WITH PHOSPHATIDYLSERINE DIACYLGLYCEROL CA2+ DETERGENT MIXED MICELLES BY CA2+-INDEPENDENT BUT NOT CA2+-DEPENDENT PROTEIN-KINASE-C ISOZYMES SO BIOCHEMISTRY LA English DT Article ID PHORBOL ESTER-BINDING; QUANTITATIVE MEASUREMENT; MEMBRANE PHOSPHOLIPIDS; MOLECULAR-CLONING; ACTIVATION; SPECIFICITY; CALCIUM; SUBSTRATE; FAMILY; BRAIN AB Mixed micelles containing Nonidet P40 (NP-40) (829 mu M or 4.8 mM), phosphatidylserine (PS) (14.5 or 8 mol %), and 1,2-diacylglycerol (DG) (0.5 or 1 mol %) when preincubated with protein kinase C (PKC) assay mixture containing cationic substrate and CaCl2 (400 mu M) formed aggregates in a time-, temperature-, and substrate concentration-dependent manner with a t(1/2) similar to 3-12 min (22 degrees C). Concomitant with the formation of these aggregates there was a substantial loss of substrate phosphorylation catalyzed by the Ca2+-dependent PKC alpha, beta, and gamma but not the Ca2+-independent PKC delta and E. All cationic PKC substrates tested, neurogranin peptide analog(29-47), neurogranin, and histone III-S, formed aggregates with PS/DG/NP-40/Ca2+ mixed micelles in a time-dependent fashion. The poly(cationic-anionic) PKC substrate protamine sulfate also forms aggregates with the mixed micelles in the presence of Ca2+, but without affecting the substrate phosphorylation by the kinase. Under similar conditions, but at 4 degrees C, neither aggregation nor loss of cationic substrate phosphorylation was observed. Another nonionic detergent, octyl glucoside, behaved similarly to NP-40. Phosphatidylinositol (PI) and phosphatidylglycerol like PS, were effective in forming aggregates with NP-40/cationic polypeptide/DG/Ca2+ as monitored by light scattering, yet without affecting substrate phosphorylation. Phosphorylation of cationic substrates by M-kinase, derived from trypsinized PKC beta, was also greatly diminished by the aggregation. In contrast, [H-3]phorbol 12,13-dibutyrate binding to PKC beta was unaffected. Formation of the aggregates that were selectively utilized by the Ca2+-independent PKCs was dependent on the ratio of cationic substrate to the number of mixed micelles. Lipid vesicles containing PS and DG or a phospholipid composition mimicking that of the cell membrane, phosphatidylcholine/phosphatidylethanolamine/sphingomyelin/PI/PS (247, 1 27, 120, 47, and 40 mu g/mL), 80 mu g/ml DG, and Ca2+, did not form such aggregates in the absence of nonionic detergent. These results indicate that the aggregation of cationic polypeptide with nonionic detergent/PS/DG/Ca2+ renders the substrate phosphorylation sites inaccessible to the Ca2+-dependent subgroup of PKCs. The current findings suggest caution in the use of cationic polypeptides that form aggregates with phospholipid/nonionic detergent/Ca2+ mixed micelles in the assay of Ca2+-dependent PKCs. C1 NICHHD,ENDOCRINOL & REPROD RES BRANCH,METAB REGULAT SECT,BETHESDA,MD 20892. NR 48 TC 11 Z9 11 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA PO BOX 57136, WASHINGTON, DC 20037-0136 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 14 PY 1995 VL 34 IS 10 BP 3446 EP 3454 DI 10.1021/bi00010a037 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QM333 UT WOS:A1995QM33300037 PM 7533539 ER PT J AU LI, X WISTOW, GJ PIATIGORSKY, J AF LI, X WISTOW, GJ PIATIGORSKY, J TI LINKAGE AND EXPRESSION OF THE ARGININOSUCCINATE LYASE DELTA-CRYSTALLIN GENES OF THE DUCK - INSERTION OF A CR-1 ELEMENT IN THE INTERGENIC SPACER SO BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION LA English DT Article DE ARGININOSUCCINATE LYASE; DELTA-CRYSTALLIN; CR-1; GENE EXPRESSION; LENS; ENHANCER; (DUCK) ID CHICKEN DELTA-1-CRYSTALLIN GENE; LENS CRYSTALLINS; NONSPECIFIC ELEMENTS; ENHANCER ELEMENTS; NONLENS TISSUES; MESSENGER-RNA; SEQUENCE; DNA; RECRUITMENT; BINDING AB delta-Crystallin is the major component of the lenses of most birds and reptiles. In the chicken there are two closely linked, tandemly oriented genes. Almost all of the delta-crystallin of the embryonic chicken lens is produced by the 5' delta 1 gene. This high lens activity has been attributed to an enhancer in intron 3. The 3' delta 2 gene encodes the enzyme argininosuccinate lyase (ASL) which is expressed at a low level in the chicken lens. Both chicken delta-crystallin genes are also expressed slightly in heart and brain, with ASL/delta 2 predominating over delta 1. In the duck (Anas platyrhynchos), ASL/delta 2-crystallin serves as both enzyme and crystallin, resulting in very high levels of ASL activity in the lens. Here we show by genomic cloning that the ASL/delta-crystallin locus is highly conserved between duck and chicken, with the two duck delta-crystallin genes closely linked in tandem. The 4.6 kbp intergenic spacer in the duck locus is 79% identical to the 4 kbp chicken spacer, except for the existence of a 615 bp CR1 element, highly reiterated in the duck genome, 1.8 kbp upstream of the duck ASL/delta 2 gene. The CRI sequence is a truncated LINE element containing the 3' half of an open reading frame for a retroviral pol-reverse transcriptase. Sequence analysis revealed (i) that intron 3 of the duck ASL/delta 2 gene is very similar (80%) to intron 3 of the chicken delta 1 and ASL/delta 2 gene, especially in the region of the chicken delta 1 enhancer core (93% identical) and (ii) that the 3' boundary of exon 2 of the duck ASL/delta 2 gene has undergone a recent splice-site slippage event, resulting in a two amino acid insertion in the encoded polypeptide. Finally, reverse transcription/polymerase chain reaction experiments established that both delta-crystallin genes are equally expressed to a high level in the embryonic duck lens; by contrast, both delta-crystallin genes produce a low amount of mRNA in the heart and brain of the embryonic duck, with the enzymatically active ASL/delta 2 being preferentially expressed. C1 NEI,MOLEC GENET SECT,MOLEC & DEV BIOL LAB,BETHESDA,MD 20892. NEI,MOLEC STRUCT & FUNCT SECT,MOLEC & DEV BIOL LAB,BETHESDA,MD 20892. NR 50 TC 8 Z9 9 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-4781 J9 BBA-GENE STRUCT EXPR JI Biochim. Biophys. Acta-Gene Struct. Expression PD MAR 14 PY 1995 VL 1261 IS 1 BP 25 EP 34 DI 10.1016/0167-4781(94)00211-K PG 10 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA QM534 UT WOS:A1995QM53400003 PM 7893758 ER PT J AU DUNCAN, MK ROTH, HJ THOMPSON, M KANTOROW, M PIATIGORSKY, J AF DUNCAN, MK ROTH, HJ THOMPSON, M KANTOROW, M PIATIGORSKY, J TI CHICKEN BETA-B1 CRYSTALLIN - GENE SEQUENCE AND EVIDENCE FOR FUNCTIONAL CONSERVATION OF PROMOTER ACTIVITY BETWEEN CHICKEN AND MOUSE SO BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION LA English DT Article DE CRYSTALLIN; IN VIVO FOOTPRINTING; TRANSGENIC MOUSE; GENE REGULATION; LENS; EYE ID LENS-SPECIFIC EXPRESSION; TRANSGENIC MICE; DELTA-CRYSTALLIN; GLOBIN GENE; PROTEINS; EVOLUTION; ENHANCER; CELLS; FAMILY; REGION AB The complete sequence was determined for the chicken beta B1-crystallin gene and 2.2 kbp of its 5' flanking region; the chicken gene was then compared to its rat orthololog. Although both have a 5' non-coding exon followed by 5 protein coding exons, the chicken gene is only 2.2 kbp while the rat gene is 13.6 kpb due to longer introns. The coding exons of the chicken beta B1-crystallin gene, like those or the rat and other beta-crystallin genes, each correspond to one of the four 'Greek key' motifs of the encoded protein. The only obvious similarity between the 5' flanking sequences of the chicken and rat beta B1-crystallin gene is associated with the TATA box. A CR1 repetitive element is present at positions -559 to -730 of the chicken beta B1-crystallin gene. In vivo footprinting using dimethyl sulfate/ligation mediated PCR showed that the PL-1 (-116/-102), PL-2 (-90/-76), OL-2 (-75/-68) and OL-1 (-125/-118) control elements identified previously (Roth et al. (1991) Mol. Cell. Biol. 11, 1488-1499) bind proteins within the chromatin of cultured embryonic chicken lens cells. Both -2448/+30 and -434/+30 promoter fragments from the chicken beta B1-crystallin gene directed lens-specific CAT gene expression in a copy number and position independent manner in transgenic mice. These data indicate that the structure and lens-specific expression of this gene are highly conserved although, like other crystallin genes, the 5' flanking sequences have diverged appreciably during evolution. C1 NEI, MOLEC & DEV BIOL LAB, BETHESDA, MD 20892 USA. NR 33 TC 36 Z9 37 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-4781 J9 BBA-GENE STRUCT EXPR JI Biochim. Biophys. Acta-Gene Struct. Expression PD MAR 14 PY 1995 VL 1261 IS 1 BP 68 EP 76 DI 10.1016/0167-4781(94)00223-P PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA QM534 UT WOS:A1995QM53400007 PM 7893762 ER PT J AU CORYELL, W ENDICOTT, J MASER, JD MUELLER, T LAVORI, P KELLER, M AF CORYELL, W ENDICOTT, J MASER, JD MUELLER, T LAVORI, P KELLER, M TI THE LIKELIHOOD OF RECURRENCE IN BIPOLAR AFFECTIVE-DISORDER - THE IMPORTANCE OF EPISODE RECENCY SO JOURNAL OF AFFECTIVE DISORDERS LA English DT Article ID PROSPECTIVE FOLLOW-UP; MANIA; PROPHYLAXIS; LITHIUM; CARBAMAZEPINE; DEPRESSION; UNIPOLAR AB These analyses used a high-intensity follow-up of of patients with bipolar affective disorder to describe the immediate and long-term risks for recurrence and the importance of sustained recovery to those risks. At the baseline evaluation, all patients were in episodes of Research Diagnostic Criteria major depressive disorder, mania or schizoaffective disorder (excluding the mainly schizophrenic subtype); those who were depressed at intake had a history of mania or schizoaffective mania. Raters re-evaluated these patients at 6-month intervals for 5 years and annually for the remainder of a 10-year follow-up. The following report describes relapse risks for the 186 patients observed to recover from their index episodes. Survival analyses quantified the likelihood of relapse over time, beginning after symptom-free periods of 4 months and 1, 2 and 3 years. Further survival analyses used treatment status as a censoring variable to estimate the eventual likelihood of recurrence among those who reported sustained compliance with lithium prophylaxis; the prophylaxis group remained under observation until they relapsed, were lost to follow-up or ceased taking lithium. Progressively longer symptom-free periods were clearly associated with lower relapse risks over the subsequent 4 years. Thereafter, however, this effect dissipitated. 7 years after recovery, the cumulative likelihood of recurrence was four in five for all bipolar patients and two in three for those whose index episode had been followed by at least 3 years without symptoms. Even with sustained lithium prophylaxis, the likelihood of at least one recurrence exceeded 70% within 5 years of recovery. The preexisting length of symptom-free periods in bipolar affective disorder can be used to predict risks for recurrence over a subsequent 4-year period. The eventual likelihood of recurrence remains quite high though, even with lithium prophylaxis. RP CORYELL, W (reprint author), NIMH,COLLABORAT PROGRAM,DEPT PSYCHIAT,2887 JPP,200 HAWKINS DR,IOWA CITY,IA 52242, USA. FU NIMH NIH HHS [R01 MH025478] NR 20 TC 44 Z9 44 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-0327 J9 J AFFECT DISORDERS JI J. Affect Disord. PD MAR 14 PY 1995 VL 33 IS 3 BP 201 EP 206 DI 10.1016/0165-0327(94)00091-M PG 6 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA QM441 UT WOS:A1995QM44100009 PM 7790673 ER PT J AU KEOWN, MB GHIRLANDO, R YOUNG, RJ BEAVIL, AJ OWENS, RJ PERKINS, SJ SUTTON, BJ GOULD, HJ AF KEOWN, MB GHIRLANDO, R YOUNG, RJ BEAVIL, AJ OWENS, RJ PERKINS, SJ SUTTON, BJ GOULD, HJ TI HYDRODYNAMIC STUDIES OF A COMPLEX BETWEEN THE FC FRAGMENT OF HUMAN IGE AND A SOLUBLE FRAGMENT OF THE FC-EPSILON-RI ALPHA-CHAIN SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE STOICHIOMETRY; HYDRODYNAMIC MODELING; ALLERGY ID HIGH-AFFINITY RECEPTOR; IMMUNOGLOBULIN-E; BINDING-SITE; NEUTRON-SCATTERING; CELL-RECEPTOR; SUBUNIT; PROTEIN; RAT; CONFORMATIONS; DOMAIN AB The interaction between immunoglobulin E (IgE) and its high-affinity receptor Fc epsilon RI is central to allergic disease. The binding site for Fc epsilon RI lies in the third constant region domain of the E heavy chain of IgE (C epsilon 3). Identical epitopes on the two C epsilon 3 domains in the IgE-Fc are predicted to be on opposite sides of the structure, and therefore each could bind independently to a receptor molecule. Titrations, however, reveal that the IgE-Fc forms an equimolar complex with a soluble fragment of the Fc epsilon RI alpha chain (sFc epsilon RI alpha), and the molecular weight of the complex, as determined by sedimentation equilibrium, confirms this stoichiometry. The measured sedimentation coefficients of the two ligands are in good agreement with computed values for a compact IgE-Fc and an elongated sFc epsilon RI alpha structure. The calculated sedimentation coefficients for possible models of a 1:1 complex lead to exclusion of all highly extended geometries for the complex. Possible explanations for the paradoxical stoichiometry of the IgE-Fc/sFc epsilon RI alpha complex, in terms of the curved shape of IgE, a conformational change in IgE when the receptor binds, and steric interference between two molecules of Fc epsilon RI binding to identical sites, are discussed. C1 UNIV LONDON KINGS COLL, RANDALL INST, LONDON WC2B 5RL, ENGLAND. NIDDKD, BETHESDA, MD 20892 USA. CELLTECH LTD, SLOUGH SL1 4EN, BERKS, ENGLAND. ROYAL FREE HOSP, SCH MED, DEPT BIOCHEM & MOLEC BIOL, LONDON NW3 2PF, ENGLAND. RI Ghirlando, Rodolfo/A-8880-2009; Beavil, Andrew/B-5624-2009 OI Beavil, Andrew/0000-0002-0768-122X FU Wellcome Trust NR 32 TC 32 Z9 32 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 14 PY 1995 VL 92 IS 6 BP 1841 EP 1845 DI 10.1073/pnas.92.6.1841 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QM408 UT WOS:A1995QM40800012 PM 7892188 ER PT J AU LAUE, L CHAN, WY HSUEH, AJW KUDO, M HSU, SY WU, SM BLOMBERG, LA CUTLER, GB AF LAUE, L CHAN, WY HSUEH, AJW KUDO, M HSU, SY WU, SM BLOMBERG, LA CUTLER, GB TI GENETIC-HETEROGENEITY OF CONSTITUTIVELY ACTIVATING MUTATIONS OF THE HUMAN LUTEINIZING-HORMONE RECEPTOR IN FAMILIAL MALE-LIMITED PRECOCIOUS PUBERTY SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID SEXUAL PRECOCITY; ACTIVE MUTANTS; CELLS; SPIRONOLACTONE; GONADOTROPIN; TESTOLACTONE; EXPRESSION; LEYDIG; OPSIN; RAT AB Genomic DNA from 32 unrelated families with male-limited precocious puberty was examined for the previously described Asp-578 --> Gly, Met-571 --> Ile, and Thr-577 --> Ile mutations in transmembrane helix 6 of the human luteinizing hormone receptor (hLHR). Twenty-eight families had the inherited form of the disorder, and of these, 24 were found to have the Asp-578 --> Gly mutation. Four additional mutations were found among the remaining four families with the inherited form and in four sporadic cases of the disorder: an A --> C transversion resulting in substitution or leucine for Ile-542 in the fifth transmembrane helix, an A --> G transition resulting in substitution of glycine for Asp-564 in the third cytoplasmic loop, a G --> T transversion resulting in substitution of tyrosine for Asp-578 in the sixth transmembrane helix, and a T --> C transition resulting in substitution of arginine for Cys-581 in the sixth transmembrane helix. Human embryonic kidney cells transfected with cDNAs for each of the mutant hLHRs, created by PCR-based mutagenesis of the wild-type hLHR cDNA, exhibited increased levels of basal cAMP production in the absence of agonist, indicating constitutive activation of the mutant hLHRs. Three of the additional mutations had specific features: Ile-542 --> Leu and Cys-581 --> Arg appeared ligand-unresponsive, whereas Asp-578 --> Tyr appeared to correlate genotype with phenotype. We conclude that the region spanning nt 1624-1741 of exon 11 is a hotspot for heterogeneous point mutations that constitutively activate the hLHR and cause male-limited precocious puberty. C1 GEORGETOWN UNIV,MED CTR,DEPT BIOCHEM & CELL BIOL,WASHINGTON,DC 20007. STANFORD UNIV,MED CTR,DEPT OBSTET & GYNECOL,STANFORD,CA 94305. NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. RP LAUE, L (reprint author), GEORGETOWN UNIV,MED CTR,DEPT PEDIAT,WASHINGTON,DC 20007, USA. FU NICHD NIH HHS [HD 32644] NR 28 TC 180 Z9 186 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 14 PY 1995 VL 92 IS 6 BP 1906 EP 1910 DI 10.1073/pnas.92.6.1906 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QM408 UT WOS:A1995QM40800025 PM 7892197 ER PT J AU HAJRA, A LIU, PP WANG, Q KELLEY, CA STACY, T ADELSTEIN, RS SPECK, NA COLLINS, FS AF HAJRA, A LIU, PP WANG, Q KELLEY, CA STACY, T ADELSTEIN, RS SPECK, NA COLLINS, FS TI THE LEUKEMIC CORE BINDING-FACTOR BETA-SMOOTH MUSCLE MYOSIN HEAVY-CHAIN (CBF-BETA-SMMHC) CHIMERIC PROTEIN REQUIRES BOTH CBF-BETA AND MYOSIN HEAVY-CHAIN DOMAINS FOR TRANSFORMATION OF NIH 3T3 CELLS (RETRACTED ARTICLE. SEE VOL 93, PG 15523, 1996) SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article; Retracted Publication ID ACUTE MYELOID-LEUKEMIA; POLYOMAVIRUS ENHANCER; NUCLEAR FACTOR; GENE; AML1; CHROMOSOME-16; ASSOCIATION; FUSION; REGION; DNA AB An inversion of chromosome 16 associated with the M4Eo subtype of acute myeloid leukemia produces a chimeric protein fusing the beta subunit of the transcription factor core binding factor (CBF beta) to the tail region of smooth muscle myosin heavy chain (SMMHC). We investigated the oncogenic properties of this CBF beta-SMMHC chimeric protein using a 3T3 transformation assay. NIH 3T3 cells expressing CBF beta-SMMHC acquired a transformed phenotype, as indicated by their ability to form foci, grow in soft agarose, and form tumors in nude mice. Cells expressing normal CBF beta or the SMMHC tail domain did not become transformed. Electrophoretic mobility-shift assays showed that extracts from cells transformed by CBF beta-SMMHC no longer formed the normal CBF/DNA complex but instead formed a much larger complex that did not migrate into the gel, Analysis of CBF beta-SMMHC deletion mutants demonstrated that the chimeric protein was transforming only if two domains were both present: (i) CBF beta sequences necessary for association with the CBF alpha subunit, and (ii) SMMHC sequences important for the formation of multimeric filaments, These results are direct evidence that CBF beta-SMMHC can function as an oncoprotein. C1 NIH, NATL CTR HUMAN GENOME RES, GENE TRANSFER LAB, BETHESDA, MD 20892 USA. UNIV MICHIGAN, SCH MED, DEPT HUMAN GENET, ANN ARBOR, MI 48109 USA. DARTMOUTH COLL, SCH MED, DEPT BIOCHEM, HANOVER, NH 03755 USA. NHLBI, MOLEC CARDIOL LAB, BETHESDA, MD 20892 USA. RI Liu, Paul/A-7976-2012 OI Liu, Paul/0000-0002-6779-025X FU NCI NIH HHS [CA58343-01] NR 33 TC 27 Z9 27 U1 0 U2 3 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 14 PY 1995 VL 92 IS 6 BP 1926 EP 1930 DI 10.1073/pnas.92.6.1926 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QM408 UT WOS:A1995QM40800029 PM 7892201 ER PT J AU SLEDJESKI, D GOTTESMAN, S AF SLEDJESKI, D GOTTESMAN, S TI SMALL RNA ACTS AS AN ANTISILENCER OF THE H-NS-SILENCED RCSA GENE OF ESCHERICHIA-COLI SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE DSRA; LON; TRANSCRIPTION INITIATION; CAPSULE SYNTHESIS ID CAPSULAR POLYSACCHARIDE SYNTHESIS; CURVED DNA; PROTEIN; EXPRESSION; OPERON; THERMOREGULATION; BACTERIOPHAGE-T4; GROWTH; K-12; K12 AB The regulation of capsular polysaccharide synthesis in Escherichia coli K-12 depends on the level of an unstable positive regulator, RcsA. The amount of RcsA protein is limited both by its rapid degradation by Lon, an ATP-dependent protease, and by its low level of synthesis. We have found that the low level of expression from the rcsA promoter is due to transcriptional silencing by the histone-like protein H-NS; this silencing is sensitive to both sequence and context in a region upstream of the -35 region of the promoter. A small (85-nt) RNA, DsrA, when overproduced, activates transcription of rcsA::lacZ fusions by counteracting H-NS silencing. DsrA RNA does not show any extended homology with the rcsA promoter or other sequenced regions of E. coli. Since the stimulation of rcsA transcription by this small RNA does not depend on any sequences from within the rcsA transcript, DsrA acts, either directly or indirectly, on rcsA transcription initiation. C1 NCI, MOLEC BIOL LAB, BETHESDA, MD 20892 USA. OI Sledjeski, Darren/0000-0001-9882-6625 FU NIGMS NIH HHS [F32GM14776-01] NR 47 TC 157 Z9 163 U1 1 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 14 PY 1995 VL 92 IS 6 BP 2003 EP 2007 DI 10.1073/pnas.92.6.2003 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QM408 UT WOS:A1995QM40800045 PM 7534408 ER PT J AU KIMURA, Y STADTMAN, TC AF KIMURA, Y STADTMAN, TC TI GLYCINE REDUCTASE SELENOPROTEIN-A IS NOT A GLYCOPROTEIN - THE POSITIVE PERIODIC ACID SCHIFF REAGENT TEST IS THE RESULT OF PEPTIDE-BOND CLEAVAGE AND CARBONYL GROUP GENERATION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE PERIODIC ACID OXIDATION; GLYCOPROTEIN ANALYSIS ID PURIFICATION; COMPLEX; SELENOCYSTEINE; COMPONENT; SEQUENCE; PROTEINS; SYSTEM; GENE AB The complete amino acid sequence of Clostridium sticklandii selenoprotein A, a selenocysteine-containing protein component of the glycine reductase complex, has been established. Both the intact protein and peptide fragments produced by Staphylococcus aureus V8 protease or trypsin were purified by reversed-phase high-performance liquid chromatography and subjected to electrospray ionization mass spectrometric analysis and standard Edman degradation. Selenoprotein A consists of 157 amino acids with a chemical molecular weight of 17,011, in reasonable agreement with the observed molecular weight (17,022.7) determined from its ionization mass spectrum. The sequence of the amino-terminal region of the isolated native protein is Ser-Arg-Phe-Thr-Gly-Lys-Lys-Ile-Val-Ile-Ile-Gly-Asp-Arg-Asp-. An N-terminal methionine residue deduced from the gene sequence was not present. Although selenoprotein A reacted positively in a glycoprotein stain when using either the periodic acid-Schiff reagent procedure or a commercial glycan detection kit, no saccharide was detected by carbohydrate analyses after acid hydrolysis or methanolysis. Identity of the amino acid sequence determined by analysis with that deduced from the gene sequence is further evidence of the absence of bound carbohydrate. C1 NHLBI,IR,BIOCHEM LAB,BETHESDA,MD 20892. NR 19 TC 16 Z9 18 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 14 PY 1995 VL 92 IS 6 BP 2189 EP 2193 DI 10.1073/pnas.92.6.2189 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QM408 UT WOS:A1995QM40800083 PM 7892245 ER PT J AU SHIRASAKA, T KAVLICK, MF UENO, T GAO, WY KOJIMA, E ALCAIDE, ML CHOKEKIJCHAI, S ROY, BM ARNOLD, E YARCHOAN, R MITSUYA, H AF SHIRASAKA, T KAVLICK, MF UENO, T GAO, WY KOJIMA, E ALCAIDE, ML CHOKEKIJCHAI, S ROY, BM ARNOLD, E YARCHOAN, R MITSUYA, H TI EMERGENCE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 VARIANTS WITH RESISTANCE TO MULTIPLE DIDEOXYNUCLEOSIDES IN PATIENTS RECEIVING THERAPY WITH DIDEOXYNUCLEOSIDES SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE MULTIDRUG RESISTANCE; REVERSE TRANSCRIPTASE; DNTP-BINDING SITE ID HIV-1 REVERSE-TRANSCRIPTASE; ZIDOVUDINE AZT; SENSITIVITY; INHIBITORS; INFECTION; MUTATION AB A set of mutations [Ala-62 --> Val(A62V), V75I, F77L, F116Y, and Q151M] in the polymerase domain of reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) confers on the virus a reduced sensitivity to multiple antiretroviral dideoxynucleosides and has been seen in HIV-1 variants isolated from patients receiving combination chemotherapy with 3'-azido-3'-deoxythymidine (AZT) plus 2',3'-dideoxycytidine (ddC) or 2',3'-dideoxyinosine (ddI). The IC50 values of AZT, ddC, ddI, 2',3'-dideoxyguanosine, and 2',3'-didehydro-3'-deoxythymidine against an infectious clone constructed to include the five mutations were significantly higher than those of a wild-type infectious clone. The K-i value for AZT 5'-triphosphate determined for the virus-associated RT from a posttherapy strain was 35-fold higher than that of RT from a pretherapy strain. Detailed analysis of HIV-1 strains isolated at various times during therapy showed that the Q151M mutation developed first in vivo, at the time when the viremia level suddenly increased, followed by the F116Y and F77L mutations. All five mutations ultimately developed, and the viremia level rose even further. Analyses based on the three-dimensional structure of HIV-1 RT suggest that the positions where at least several of the five mutations occur are located in close proximity to the proposed dNTP-binding site of RT and the first nucleotide position of the single-stranded template. C1 NCI,MED BRANCH,EXPTL RETROVIROL SECT,BETHESDA,MD 20892. NCI,RETROVIRAL DIS SECT,BETHESDA,MD 20892. RUTGERS STATE UNIV,CTR ADV BIOTECHNOL & MED,PISCATAWAY,NJ 08854. RUTGERS STATE UNIV,DEPT CHEM,PISCATAWAY,NJ 08854. RI Ueno, Takamasa/F-5788-2013 OI Ueno, Takamasa/0000-0003-4852-4236 FU NIAID NIH HHS [AI27690, AI36144] NR 30 TC 338 Z9 342 U1 0 U2 3 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 14 PY 1995 VL 92 IS 6 BP 2398 EP 2402 DI 10.1073/pnas.92.6.2398 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QM408 UT WOS:A1995QM40800126 PM 7534421 ER PT J AU ANDERSSON, HC PARRY, DM MULVIHILL, JJ AF ANDERSSON, HC PARRY, DM MULVIHILL, JJ TI LYMPHANGIOSARCOMA IN LATE-ONSET HEREDITARY LYMPHEDEMA - CASE-REPORT AND NOSOLOGICAL IMPLICATIONS SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE HEREDITARY LYMPHEDEMA; LYMPHANGIOSARCOMA; MEIGE DISEASE AB Hereditary lymphedemas that are not associated with other malformations usually affect the lower limbs and are inherited in an autosomal dominant fashion. These nonsyndromic hereditary lymphedemas are categorized by their age of onset, being either congenital (Milroy disease) or having an onset in childhood or around puberty (Meige disease). We describe a family in which three individuals in three generations had unusually late onset of lymphedema in their mid-twenties or thirties. The proband additionally developed a very rare lymphangiosarcoma. This tumor, usually associated with post-mastectomy lymphedema, has not been described in late-onset hereditary lymphedema. Because of an unusually high incidence of multiple primary tumors in association with lymphangiosarcoma in the literature (approximately 10%) and proband's own familial cancer background, we speculate that an inherited predisposition to malignancy may underlie the development of lymphedema-associated lymphangiosarcoma. (C) 1995 Wiley-Liss, Inc. C1 NCI,INTERINST MED GENET PROGRAM,BETHESDA,MD 20892. NCI,CLIN EPIDEMIOL BRANCH,BETHESDA,MD 20892. UNIV PITTSBURGH,DEPT HUMAN GENET,PITTSBURGH,PA. RP ANDERSSON, HC (reprint author), TULANE UNIV,MED CTR,HAYWARD GENET CTR,1430 TULANE AVE,NEW ORLEANS,LA 70112, USA. NR 19 TC 10 Z9 11 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD MAR 13 PY 1995 VL 56 IS 1 BP 72 EP 75 DI 10.1002/ajmg.1320560116 PG 4 WC Genetics & Heredity SC Genetics & Heredity GA QJ893 UT WOS:A1995QJ89300015 PM 7747790 ER PT J AU MILLER, RW RUBINSTEIN, JH AF MILLER, RW RUBINSTEIN, JH TI TUMORS IN RUBINSTEIN-TAYBI SYNDROME SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE RUBINSTEIN-TAYBI SYNDROME; NEOPLASMS; BRAIN TUMORS; GERM CELL TUMORS; CONGENITAL ANOMALIES ID CONGENITAL-ANOMALIES AB The 14 tumors reported in Rubinstein-Taybi syndrome since 1989, when added to the 22 previously reported, are beginning to show a pattern of neural and developmental tumors, especially of the head, which is malformed in the syndrome. Among the neoplasms were 12 of the nervous systems: 2 each of oligodendroglioma, medulloblastoma, neuroblastoma, and benign meningioma, a pheochromocytoma, and 3 other benign tumors; 2 of nasopharyngeal rhabdomyosarcoma; and 1 each of leiomyosarcoma, seminoma, and embryonal carcinoma. Among the other benign tumors were an odontoma, a choristoma, a dermoid cyst, and 2 pilomatrixomas. (C) 1995 Wiley-Liss, Inc. C1 UNIV CINCINNATI,AFFILIATED CTR DEV DISORDERS,CINCINNATI,OH. UNIV CINCINNATI,COLL MED,DEPT PEDIAT,CINCINNATI,OH. CHILDRENS HOSP,MED CTR,CINCINNATI,OH 45229. RP MILLER, RW (reprint author), NCI,CLIN EPIDEMIOL BRANCH,EPN 400,BETHESDA,MD 20892, USA. FU PHS HHS [MCJ-399156-04-1] NR 20 TC 164 Z9 172 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD MAR 13 PY 1995 VL 56 IS 1 BP 112 EP 115 DI 10.1002/ajmg.1320560125 PG 4 WC Genetics & Heredity SC Genetics & Heredity GA QJ893 UT WOS:A1995QJ89300024 PM 7747773 ER PT J AU MEMON, SA PETRAK, D MORENO, MB ZACHARCHUK, CM AF MEMON, SA PETRAK, D MORENO, MB ZACHARCHUK, CM TI A SIMPLE ASSAY FOR EXAMINING THE EFFECT OF TRANSIENTLY EXPRESSED GENES ON PROGRAMMED CELL-DEATH SO JOURNAL OF IMMUNOLOGICAL METHODS LA English DT Article DE APOPTOSIS; BCL-2; TRANSIENT TRANSFECTION ID MYELOID LEUKEMIC-CELLS; INDUCED APOPTOSIS; C-MYC; THYMOCYTE APOPTOSIS; BETA-GALACTOSIDASE; TRANSGENIC MICE; CYCLE BLOCK; BCL-2; ACTIVATION; P53 AB Programmed cell death (PCD) has been observed in a wide variety of cell types in response to physiologic signals or types of stress. How these stimuli trigger PCD, and whether there is a common PCD signal transduction pathway, is not clear. As more genes are described that may participate in or regulate PCD, an assay system in which gene products can easily be introduced and/or modulated would be of great value. To avoid the generation and screening of multiple individual stable cell transfectants, a simple transient transfection death assay has been developed. 2B4.11, a murine T cell hybridoma, was transfected by electroporation with a constitutively active P-galactosidase reporter gene and the cells were incubated in culture medium or with a PCD-inducing stimulus. The amount of P-galactosidase activity remaining in the intact cells at the end of the culture period represented only viable transfected cells. Bcl-2 was chosen to examine whether this system would be useful to study the effect of transiently transfected genes since it blocks PCD in a number of experimental systems. Consistent with data obtained using stable transfectants, transient expression of Bcl-2 in 2B4.11 completely protected cells from glucocorticoid- and cytotoxic agent-induced PCD. This protection from death was confirmed at the individual cell level by the transient co-expression of a class I L(d) surface antigen and flow cytometric analysis. Some of the advantages of the transient transfection death assay described here are; (1) the simple and sensitive beta-galactosidase assay, (2) the rapidity of the assay, (3) the ability to perform conventional viability assays to monitor treatment-induced cytotoxicity, (4) multiple gene products can be tested alone, and in combination, (5) antisense or dominant negative approaches can be used, and (6) the adaptability of this assay system to other cell types, transfection techniques, or reporter and expression vectors. The transient transfection death assay should make it easier to identify and order important steps in the PCD signal transduction pathways. C1 NCI,IMMUNE CELL BIOL LAB,BIOL RESPONSE MODIFIERS PROGRAM,BETHESDA,MD 20892. RI Memon, Sarfraz/E-1198-2013 NR 40 TC 33 Z9 33 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1759 J9 J IMMUNOL METHODS JI J. Immunol. Methods PD MAR 13 PY 1995 VL 180 IS 1 BP 15 EP 24 DI 10.1016/0022-1759(94)00294-7 PG 10 WC Biochemical Research Methods; Immunology SC Biochemistry & Molecular Biology; Immunology GA QM770 UT WOS:A1995QM77000002 PM 7897244 ER PT J AU MAGRATH, I AF MAGRATH, I TI BONE-MARROW TRANSPLANTATION FOR LEUKEMIA - A LAME STALKING HORSE FOR USE OF HIGH-TECHNOLOGY MEDICAL-CARE SO LANCET LA English DT Editorial Material RP MAGRATH, I (reprint author), NIH,LYMPHOMA BIOL SECT,BETHESDA,MD 20892, USA. NR 6 TC 4 Z9 4 U1 1 U2 1 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0099-5355 J9 LANCET JI Lancet PD MAR 11 PY 1995 VL 345 IS 8950 BP 601 EP 602 DI 10.1016/S0140-6736(95)90516-2 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA QM075 UT WOS:A1995QM07500004 PM 7898174 ER PT J AU STOLOW, DT HAYNES, SR AF STOLOW, DT HAYNES, SR TI CABEZA, A DROSOPHILA GENE ENCODING A NOVEL RNA-BINDING PROTEIN, SHARES HOMOLOGY WITH EWS AND TLS, 2 GENES INVOLVED IN HUMAN SARCOMA FORMATION SO NUCLEIC ACIDS RESEARCH LA English DT Article ID NUCLEAR RIBONUCLEOPROTEIN PARTICLE; HEAD-SPECIFIC GENES; ON-TRANSIENT-A; MOLECULAR ANALYSIS; SEX-LETHAL; CONSENSUS SEQUENCE; RIBOSOMAL-PROTEINS; PREMESSENGER RNA; X-CHROMOSOME; EXPRESSION AB We have previously described a partial Drosophila cDNA, clone P19, which bears homology to members of the RNA recognition motif (RRM) family of proteins [Haynes et al. (1987) Proc. Natl. Acad. Sci. USA, 84, 1819-1823]. RNA binding as well as involvement in RNA processing has been demonstrated for some RRM proteins. We report here the further characterization of P19, which we renamed cabeza (caz). caz is located on the X chromosome at position 14B. Using Northern analysis, at least four transcripts from the caz gene were observed at varying revels during development, caz mRNA and protein are enriched in the brain and central nervous system during embryogenesis. In addition, the protein is enriched in the adult head. UV crosslinking was used to demonstrate in vitro RNA binding activity for full-length recombinant caz protein and for the caz RRM domain. Sequence analysis revealed caz is related to two human genes, EWS and TLS, which are involved in chromosomal translocations. The fusion of EWS and TLS to other cellular genes results in sarcoma formation. In addition to their overall structural organization and sequence similarity, these three genes share an RRM which is divergent from typical RRMs. Therefore, it appears that these genes constitute a new sub-family of RNA binding proteins. C1 NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. NR 69 TC 43 Z9 47 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD MAR 11 PY 1995 VL 23 IS 5 BP 835 EP 843 DI 10.1093/nar/23.5.835 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ184 UT WOS:A1995QQ18400018 PM 7708500 ER PT J AU VOELLER, DM CHANGCHIEN, LM MALEY, GF MALEY, F TAKECHI, T TURNER, RE MONTFORT, WR ALLEGRA, CJ CHU, E AF VOELLER, DM CHANGCHIEN, LM MALEY, GF MALEY, F TAKECHI, T TURNER, RE MONTFORT, WR ALLEGRA, CJ CHU, E TI CHARACTERIZATION OF A SPECIFIC INTERACTION BETWEEN ESCHERICHIA-COLI THYMIDYLATE SYNTHASE AND ESCHERICHIA-COLI THYMIDYLATE SYNTHASE MESSENGER-RNA SO NUCLEIC ACIDS RESEARCH LA English DT Article ID MESSENGER-RNA TRANSLATION; 3' UNTRANSLATED REGION; BINDING-SITE; NUCLEOTIDE-SEQUENCE; DNA-POLYMERASE; PROTEIN; IDENTIFICATION; FERRITIN; INVITRO; ENZYME AB Previous studies have shown that human TS mRNA translation is controlled by a negative autoregulatory mechanism. In this study, an RNA electrophoretic gel mobility shift assay confirmed a direct interaction between Escherichia coli (E.coli) TS protein and its own E.coli TS mRNA. Two cis-acting sequences in the E.coli TS mRNA protein-coding region were identified, with one site corresponding to nucleotides 207-460 and the second site corresponding to nucleotides 461-807. Each of these mRNA sequences bind TS with a relative affinity similar to that of the full-length E.coli TS mRNA sequence (IC50 = 1 nM). A third binding site was identified, corresponding to nucleotides 808-1015, although its relative affinity for TS (IC50 = 5.1 nM) was lower than that of the other two cis-acting elements. E.coli TS proteins with mutations in amino acids located within the nucleotide-binding region retained the ability to bind RNA while proteins with mutations at either the nucleotide active site cysteine (C146S) or at amino acids located within the folate-binding region were unable to bind TS mRNA. These studies suggest that the regions on E.coli TS defined by the folate-binding site and/or critical cysteine sulfhydryl groups may represent important RNA binding domains. Further evidence is presented which demonstrates that the direct interaction with TS results in in vitro repression of E.coli TS mRNA translation. C1 NCI, USN, MED ONCOL BRANCH, BETHESDA, MD 20889 USA. NEW YORK STATE DEPT HLTH, WADSWORTH CTR, ALBANY, NY 12201 USA. UNIV ARIZONA, DEPT BIOCHEM, TUCSON, AZ 85721 USA. FU NCI NIH HHS [CA44355] NR 45 TC 23 Z9 24 U1 0 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 EI 1362-4962 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD MAR 11 PY 1995 VL 23 IS 5 BP 869 EP 875 DI 10.1093/nar/23.5.869 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QQ184 UT WOS:A1995QQ18400023 PM 7708505 ER PT J AU CURRAN, ME SPLAWSKI, I TIMOTHY, KW VINCENT, GM GREEN, ED KEATING, MT AF CURRAN, ME SPLAWSKI, I TIMOTHY, KW VINCENT, GM GREEN, ED KEATING, MT TI A MOLECULAR-BASIS FOR CARDIAC-ARRHYTHMIA - HERG MUTATIONS CAUSE LONG QT SYNDROME SO CELL LA English DT Article ID EARLY AFTERDEPOLARIZATIONS; GENE; DNA; LINKAGE; IDENTIFICATION; HETEROGENEITY; FRAGMENTS; CHANNEL; LOCUS; DEATH AB To identify genes involved in cardiac arrhythmia, we investigated patients with long QT syndrome (LQT), an inherited disorder causing sudden death from a ventricular tachyarrhythmia, torsade de pointes. We previously mapped LQT loci on chromosomes 11 (LQT1), 7(LQT2), and 3(LQT3). Here, linkage and physical mapping place LQT2 and a putative potassium channel gene, HERG, on chromosome 7q35-36. Single strand conformation polymorphism and DNA sequence analyses reveal HERG mutations in six LQT families, including two intragenic deletions, one splice-donor mutation, and three missense mutations. In one kindred, the mutation arose de novo. Northern blot analyses show that HERG is strongly expressed in the heart. These data indicate that HERG is LQT2 and suggest a likely cellular mechanism for torsade de pointes. C1 UNIV UTAH,HLTH SCI CTR,ECCLES PROGRAM HUMAN MOLEC BIOL & GENET,SALT LAKE CITY,UT 84112. UNIV UTAH,HLTH SCI CTR,DIV CARDIOL,SALT LAKE CITY,UT 84112. UNIV UTAH,HLTH SCI CTR,HOWARD HUGHES MED INST,SALT LAKE CITY,UT 84112. LATTER DAY ST HOSP,DEPT MED,SALT LAKE CITY,UT 84037. NIH,NATL CTR HUMAN GENOME RES,BETHESDA,MD 20892. RP CURRAN, ME (reprint author), UNIV UTAH,HLTH SCI CTR,DEPT HUMAN GENET,SALT LAKE CITY,UT 84112, USA. FU NCRR NIH HHS [MO1-RR00064]; NHLBI NIH HHS [R01-HL 48074] NR 38 TC 1546 Z9 1589 U1 8 U2 68 PU CELL PRESS PI CAMBRIDGE PA 50 CHURCH ST CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD MAR 10 PY 1995 VL 80 IS 5 BP 795 EP 803 DI 10.1016/0092-8674(95)90358-5 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QM399 UT WOS:A1995QM39900016 PM 7889573 ER PT J AU ABRAMS, RM BURCHFIELD, DJ SUN, Y SMITH, CB AF ABRAMS, RM BURCHFIELD, DJ SUN, Y SMITH, CB TI PRENATAL DEVELOPMENT AND LOCAL-RATES OF CEREBRAL PROTEIN-SYNTHESIS (ICPSLEU) IN SHEEP SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,CEREBRAL METAB LAB,BETHESDA,MD 20892. UNIV FLORIDA,COLL MED,DEPT OBSTET GYNECOL,GAINESVILLE,FL 32610. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A757 EP A757 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600767 ER PT J AU AMICHAY, D GAZZINELLI, R KARUPIAH, G MOENCH, T SHER, A FARBER, J AF AMICHAY, D GAZZINELLI, R KARUPIAH, G MOENCH, T SHER, A FARBER, J TI MIG IS INDUCED IN THE MOUSE BY IFN-GAMMA AND BY PROTOZOAN AND VIRAL-INFECTIONS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A783 EP A783 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600916 ER PT J AU BARNES, DM SYKES, DB MILLER, DS AF BARNES, DM SYKES, DB MILLER, DS TI INSULIN-LIKE EFFECTS OF HGCL2 ON HEXOSE-TRANSPORT IN XENOPUS OOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A635 EP A635 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600061 ER PT J AU BIANCHINE, JP PAOLINI, R KINET, JP METCALFE, DD AF BIANCHINE, JP PAOLINI, R KINET, JP METCALFE, DD TI 3 DISTINCT PATHWAYS ACTIVATE FOCAL ADHESION KINASE IN MAST-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A781 EP A781 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600901 ER PT J AU BOUCHER, W PANG, X WEBSTER, E CHROUSOS, G PAPANICOLAOU, D THEOHARIDES, TC AF BOUCHER, W PANG, X WEBSTER, E CHROUSOS, G PAPANICOLAOU, D THEOHARIDES, TC TI CORTICOTROPIN-RELEASING HORMONE (CRH) INDUCES RAT MAST-CELL (MC) SECRETION SO FASEB JOURNAL LA English DT Meeting Abstract C1 TUFTS UNIV,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,BOSTON,MA. NIH,PEDIAT ENDOCRINOL SECT,BETHESDA,MD. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A919 EP A919 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601713 ER PT J AU CHANG, AC WADSWORTH, SA HONG, MP HALVORSON, MJ OTTO, S COLIGAN, JE AF CHANG, AC WADSWORTH, SA HONG, MP HALVORSON, MJ OTTO, S COLIGAN, JE TI EXPRESSION OF A NOVEL INTEGRIN BETA(1) CHAIN EPITOPE AND ANTI-BETA(1) ANTIBODY-MEDIATED ENHANCEMENT OF FIBRONECTIN-BINDING ARE DEPENDENT ON THE STAGE OF T-CELL DIFFERENTIATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LMS,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A814 EP A814 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601100 ER PT J AU CHOUDHURY, BK HOU, EW LI, SSL AF CHOUDHURY, BK HOU, EW LI, SSL TI STRUCTURE AND EVOLUTION OF THE NEUROGENIC ENHANCER OF SPLIT GROUCHO AND RELATED GENES FROM HUMAN, MOUSE AND XENOPUS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,GENET MOLEC LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A705 EP A705 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600470 ER PT J AU CHUANG, RY CHUANG, LF KUNG, HF YU, L KILLAM, KF AF CHUANG, RY CHUANG, LF KUNG, HF YU, L KILLAM, KF TI OPIOID RECEPTOR GENE-EXPRESSION IN LYMPHOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CALIF DAVIS,DAVIS,CA 95616. NCI,FREDERICK,MD 21701. INDIANA UNIV,SCH MED,INDIANAPOLIS,IN 46202. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A850 EP A850 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601311 ER PT J AU CIPPITELLI, M SICA, A VIGGIANO, V YE, J GHOSH, P BIRRER, MJ YOUNG, HA AF CIPPITELLI, M SICA, A VIGGIANO, V YE, J GHOSH, P BIRRER, MJ YOUNG, HA TI NEGATIVE TRANSCRIPTIONAL REGULATION OF THE INTERFERON-GAMMA (IFN-GAMMA) PROMOTOR BY GLUCOCORTICOIDS AND DOMINANT-NEGATIVE MUTANTS OF C-JUN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,BCDP,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,BRMP,LEI,FREDERICK,MD 21702. NCI,DCPC,BPRB,ROCKVILLE,MD 20850. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A823 EP A823 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601149 ER PT J AU COLE, DJ WEIL, DP SHILYANSKY, J CUSTER, M KAWAKAMI, Y YANNELLI, J ROSENBERG, SA NISHIMURA, MI AF COLE, DJ WEIL, DP SHILYANSKY, J CUSTER, M KAWAKAMI, Y YANNELLI, J ROSENBERG, SA NISHIMURA, MI TI T-CELL RECEPTOR USAGE AND EPITOPE MAPPING OF HLA-A2 RESTRICTED, MELANOMA REACTIVE CTL CLONES AND OLIGOCLONAL LINES SO FASEB JOURNAL LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,DEPT SURG,CHARLESTON,SC 29425. NCI,SURG BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A801 EP A801 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601018 ER PT J AU DEBRE, P RUSCETTI, FW MOSSALAYI, J AF DEBRE, P RUSCETTI, FW MOSSALAYI, J TI EARLY HUMAN THYMOCYTES DEVELOPMENT IS REGULATED BY AN EXTERNALLY CONTROLLED AUTOCRINE TGF-BETA(1) MECHANISM SO FASEB JOURNAL LA English DT Meeting Abstract C1 HOP LA PITIE SALPETRIERE,IMMUNOL LAB,PARIS,FRANCE. NCI,LEUKOCYTE BIOL LAB,FREDERICK,MD 21701. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A815 EP A815 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601101 ER PT J AU FRAZIERJESSEN, M CHRIST, M PANEK, RB MCCARTNEYFRANCIS, N KATONA, I BAUER, S KULKARNI, A WARD, J WAHL, S AF FRAZIERJESSEN, M CHRIST, M PANEK, RB MCCARTNEYFRANCIS, N KATONA, I BAUER, S KULKARNI, A WARD, J WAHL, S TI ABERRANT B-LYMPHOCYTE FUNCTION IN TGF-BETA-1(-/-) NULL MICE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. NINCDS,BETHESDA,MD 20892. NCI,BETHESDA,MD 20892. NIH,BETHESDA,MD 20892. US FDA,BETHESDA,MD 20892. UNIFORMED SERV UNIV HLTH SCI,BETHESDA,MD 20814. NR 0 TC 3 Z9 3 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A814 EP A814 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601095 ER PT J AU FRENCH, JE NYLANDERFRENCH, LA AF FRENCH, JE NYLANDERFRENCH, LA TI SYNERGISTIC IN-VITRO CYTOTOXICITY OF UVA RADIATION AND CHEMICAL ELECTROPHILES TO NORMAL HUMAN SKIN CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 SNIOH,S-17184 SOLNA,SWEDEN. NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A713 EP A713 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600512 ER PT J AU FUNAKOSHI, S TAUB, DD ANVER, MR WEEKS, SD RAZIUDDIN, A CORRALES, SM ARMITAGE, RJ FANSLOW, WC LONGO, DL MURPHY, WJ AF FUNAKOSHI, S TAUB, DD ANVER, MR WEEKS, SD RAZIUDDIN, A CORRALES, SM ARMITAGE, RJ FANSLOW, WC LONGO, DL MURPHY, WJ TI TREATMENT OF MICE WITH SOLUBLE RECOMBINANT CD40 LIGAND PROMOTES B-CELL RECOVERY AFTER SYNGENEIC BONE-MARROW TRANSPLANTATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,DCT,LLB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,BCDP,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,LASP,FREDERICK,MD 21702. IMMUNEX CORP,SEATTLE,WA 98101. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A783 EP A783 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600917 ER PT J AU GALLAGHER, D VISSER, M HARRIS, TB HEYMSFIELD, SB AF GALLAGHER, D VISSER, M HARRIS, TB HEYMSFIELD, SB TI PROPORTION OF FAT-FREE BODY-MASS AS SKELETAL-MUSCLE MASS SO FASEB JOURNAL LA English DT Meeting Abstract C1 ST LUKES ROOSEVELT HOSP,OBES RES CTR,NEW YORK,NY 10025. AGR UNIV WAGENINGEN,DEPT HUMAN NUTR,WAGENINGEN,NETHERLANDS. NIA,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A979 EP A979 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602062 ER PT J AU HAMAWY, MM MINOGUCHI, K SWAIM, WD MERGENHAGEN, SE SIRAGANIAN, RP AF HAMAWY, MM MINOGUCHI, K SWAIM, WD MERGENHAGEN, SE SIRAGANIAN, RP TI A 77 KDA PROTEIN ASSOCIATES WITH PP125(FAK) IN MAST-CELLS BUT NOT IN FIBROBLASTS AND BECOMES PHOSPHORYLATED ON TYROSINE AFTER AGGREGATION OF THE HIGH-AFFINITY IGE RECEPTOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A781 EP A781 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600904 ER PT J AU HAMELINK, CR ESKAY, RL CASTONGUAY, TW AF HAMELINK, CR ESKAY, RL CASTONGUAY, TW TI ACCESS TO A FAT SUPPLEMENT STIMULATES HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,BETHESDA,MD. UNIV MARYLAND,COLLEGE PK,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A1005 EP A1005 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602207 ER PT J AU HARRISON, EH KEMPNER, ES AF HARRISON, EH KEMPNER, ES TI SIZE OF THE CATALYTICALLY ACTIVE UNIT OF RAT HEPATIC CARBOXYLESTER LIPASE (CEL) IN THE PRESENCE AND ABSENCE OF BILE-SALT SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAMSD,BETHESDA,MD. MED COLL PENN,PHILADELPHIA,PA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A747 EP A747 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600711 ER PT J AU HEEMSKERK, FMJ GALOIAN, KA SAAVEDRA, JM AF HEEMSKERK, FMJ GALOIAN, KA SAAVEDRA, JM TI CHARACTERIZATION AND SOLUBILIZATION OF A NOVEL RECEPTOR FOR CGP-42112 IN THE RAT SPLEEN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,CLIN SCI LAB,PHARMACOL SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A925 EP A925 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601752 ER PT J AU HERION, D RYSCHON, T ROSENSTEIN, D ELIN, R NIEMELA, J RUBINOW, D ECKARDT, M BALABAN, R AF HERION, D RYSCHON, T ROSENSTEIN, D ELIN, R NIEMELA, J RUBINOW, D ECKARDT, M BALABAN, R TI TISSUE MAGNESIUM CONCENTRATIONS IN ALCOHOLICS AND CONTROLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NIAAA,BETHESDA,MD 20892. NIMH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A870 EP A870 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601430 ER PT J AU HORNUNG, RL LONGO, DL KWAK, LW AF HORNUNG, RL LONGO, DL KWAK, LW TI T-CELL MEDIATION OF ANTIGEN-SPECIFIC IMMUNE BONE-MARROW THERAPY AGAINST AN ESTABLISHED LYMPHOMA SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,BCDP,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A799 EP A799 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601011 ER PT J AU KARANIAN, J SALEM, N AF KARANIAN, J SALEM, N TI HYDROPEROXY AND HYDROXY N-3/N-6 FATTY-ACIDS IN PLATELET AND VASCULAR SMOOTH-MUSCLE FUNCTION - INTERFERENCE WITH TXA2/PGH2 RECEPTORS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,DICBR,MEMBRANE BIOCHEM & BIOPHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A939 EP A939 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601830 ER PT J AU KING, LB HUNZIKER, R MARGULIES, DH ASHWELL, JD AF KING, LB HUNZIKER, R MARGULIES, DH ASHWELL, JD TI A TARGETED GLUCOCORTICOID RECEPTOR ANTISENSE TRANSGENE ENHANCES APOPTOTIC THYMOCYTE DELETION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BIOL RESPONSE MODIFIERS PROGRAM,IMMUNE CELL BIOL LAB,BETHESDA,MD 20892. NIAID,IMMUNOL LAB,BETHESDA,MD 20892. RI Margulies, David/H-7089-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A778 EP A778 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600888 ER PT J AU KLEINMAN, HK AF KLEINMAN, HK TI ROLE OF BASEMENT-MEMBRANE IN DIFFERENTIATION AND TUMORS GROWTH SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A695 EP A695 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600409 ER PT J AU KOLE, HK SMYTH, MS RUSS, PL BURKE, TR AF KOLE, HK SMYTH, MS RUSS, PL BURKE, TR TI PHOSPHONATE-CONTAINING INHIBITORS OF PROTEIN-TYROSINE AND SERINE THREONINE PHOSPHATASES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,DIABET UNIT,BALTIMORE,MD 21224. NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,BETHESDA,MD 20892. NCI,MED CHEM LAB,BETHESDA,MD 20892. NIA,CLIN PHYSIOL LAB,BALTIMORE,MD 21224. RI Burke, Terrence/N-2601-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A752 EP A752 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600741 ER PT J AU KWON, TK BUCHHOLZ, M GABRIELSON, E NORDIN, A AF KWON, TK BUCHHOLZ, M GABRIELSON, E NORDIN, A TI A NOVEL SUBSTRATE FOUND IN EPITHELIAL TYPE CELLS FOR CDK4 AND CDK6 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A1066 EP A1066 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602557 ER PT J AU LIU, K ROMAN, JS KAMALI, V NAUGHTON, BA KELLER, J RUSCETTI, FW ROODMAN, D BONEWALD, L MEAGHER, RM PURCHIO, AF TWARDZIK, DR AF LIU, K ROMAN, JS KAMALI, V NAUGHTON, BA KELLER, J RUSCETTI, FW ROODMAN, D BONEWALD, L MEAGHER, RM PURCHIO, AF TWARDZIK, DR TI STEM-CELL PROLIFERATION FACTOR (SCPF) INDUCES THE EXPANSION OF PRIMITIVE CD34 CELLS IN CULTURE SO FASEB JOURNAL LA English DT Meeting Abstract C1 ADV TISSUE SCI INC,LA JOLLA,CA 92037. NCI,FREDERICK,MD 21702. HOXWORTH BLOOD CTR,CINCINNATI,OH 45267. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A940 EP A940 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601840 ER PT J AU LONGMAN, RE REMMERS, EF DU, Y GRIFFITHS, M WILDER, RL AF LONGMAN, RE REMMERS, EF DU, Y GRIFFITHS, M WILDER, RL TI GENETIC-LOCI CONTROLLING COLLAGEN-INDUCED ARTHRITIS (CIA) SEVERITY IN PROGENY OF DA (SUSCEPTIBLE) AND F344 (RESISTANT) RAT STRAINS SO FASEB JOURNAL LA English DT Meeting Abstract C1 VET AFFAIRS MED CTR,RES SERV,SALT LAKE CITY,UT 84132. UNIV UTAH,DEPT MED RHEUMATOL,SALT LAKE CITY,UT 84132. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A786 EP A786 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600932 ER PT J AU MELILLO, G MUSSO, T COX, GW SICA, A SHEFFLER, LA VARESIO, L AF MELILLO, G MUSSO, T COX, GW SICA, A SHEFFLER, LA VARESIO, L TI IDENTIFICATION OF A FUNCTIONAL PICOLINIC ACID-RESPONSE ELEMENT IN THE PROMOTER OF INDUCIBLE NITRIC-OXIDE SYNTHASE (INOS) GENE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,BIOL RESPONSE MODIFIERS PROGRAM,EXPTL IMMUNOL LAB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,BCDP,FREDERICK,MD 21702. RI varesio, luigi/J-8261-2016 OI varesio, luigi/0000-0001-5659-2218 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A779 EP A779 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600890 ER PT J AU MILLER, DS VILLALOBOS, AR PRITCHARD, JB AF MILLER, DS VILLALOBOS, AR PRITCHARD, JB TI ORGANIC-BASE (QUINACRINE, QCRN) UPTAKE AND DISTRIBUTION IN RAT CHOROID-PLEXUS CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A915 EP A915 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601694 ER PT J AU MULLET, D BIAN, X COX, GW ZAVERI, N GERBER, N FERTEL, RH AF MULLET, D BIAN, X COX, GW ZAVERI, N GERBER, N FERTEL, RH TI GALLIUM NITRATE INHIBITS NITRIC-OXIDE PRODUCTION BY ACTIVATED ANA-1 MACROPHAGES SO FASEB JOURNAL LA English DT Meeting Abstract C1 OHIO STATE UNIV,COLL MED,DEPT PHARMACOL,COLUMBUS,OH 43210. PUMC,DEPT OBSTET & GYNECOL,BEIJING,PEOPLES R CHINA. NCI,FREDERICK CANC RES & DEV CTR,BIOL RESPONSE MODIFIERS PROGRAM,EXPTL IMMUNOL LAB,FREDERICK,MD 21702. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A944 EP A944 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601860 ER PT J AU MUNOZ, K BALLARDBARBASH, R SWANSON, C AF MUNOZ, K BALLARDBARBASH, R SWANSON, C TI RECALL OF BODY-WEIGHT AND BODY-SIZE ESTIMATION IN WOMEN IN THE BREAST-CANCER DEMONSTRATION PROJECT (BCDDP) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A1013 EP A1013 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602255 ER PT J AU OELTGEN, PR HORTON, ND KAFTANI, DJ SU, TP AF OELTGEN, PR HORTON, ND KAFTANI, DJ SU, TP TI BIOCHEMICAL-CHARACTERIZATION OF A HIBERNATION-SPECIFIC PROTEIN FROM THE PLASMA OF HIBERNATING WOODCHUCKS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDA,BALTIMORE,MD 21224. VET ADM MED CTR,LEXINGTON,KY 40511. UNIV KENTUCKY,COLL MED,GRAD CTR TOXICOL,DEPT PATHOL,LEXINGTON,KY 40511. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A642 EP A642 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600106 ER PT J AU OHANLON, TP RABEN, N MILLER, FW AF OHANLON, TP RABEN, N MILLER, FW TI THE HUMAN HISTIDYL-TRANSFER-RNA SYNTHETASE LOCUS BIDIRECTIONAL PROMOTER DIRECTS THE SYNTHESIS OPPOSITE-STRAND MESSENGER-RNA THAT PREDICTS A POLYPEPTIDE HOMOLOGOUS WITH HRS SO FASEB JOURNAL LA English DT Meeting Abstract C1 US FDA,CTR BIOL EVALUAT & RES,BETHESDA,MD 20892. NIAMS,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A857 EP A857 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601349 ER PT J AU ORTALDO, JR MASON, AT OSHEA, JJ AF ORTALDO, JR MASON, AT OSHEA, JJ TI RECEPTOR-INDUCED DEATH IN HUMAN NK CELLS - INVOLVEMENT OF CD16 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,BIOL RESPONSE MODIFIERS PROGRAM,LEI,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A793 EP A793 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600975 ER PT J AU PANEK, RB BRANDES, ME WAHL, SM AF PANEK, RB BRANDES, ME WAHL, SM TI CYTOKINE REGULATION OF TGF-BETA RECEPTOR EXPRESSION IN PERIPHERAL-BLOOD MONOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A1021 EP A1021 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602301 ER PT J AU PARK, CS GIANOTTI, C PARK, R KRISHNA, G AF PARK, CS GIANOTTI, C PARK, R KRISHNA, G TI MOLECULAR-CLONING AND EXPRESSION OF CONSTITUTIVE ISOFORM OF NITRIC-OXIDE SYNTHASE FROM HUMAN RETINA SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A680 EP A680 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600324 ER PT J AU PINET, V BAKKE, O LONG, EO AF PINET, V BAKKE, O LONG, EO TI ANTIGEN PRESENTATION MEDIATED BY RECYCLING OF SURFACE HLA-DR MOLECULES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOGENET LAB,ROCKVILLE,MD 20852. UNIV OSLO,DEPT BIOL,OSLO,NORWAY. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A1026 EP A1026 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602327 ER PT J AU PRITCHARD, JB AF PRITCHARD, JB TI IMPACT OF INTRACELLULAR ALPHA-KETOGLUTARATE (ALPHA-KG) CONCENTRATION AND COMPARTMENTATION ON RENAL ORGANIC ANION TRANSPORT SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A915 EP A915 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601692 ER PT J AU ROUBENOFF, R KIEL, DP HANNAN, MT DALLAL, GE WILSON, PWF HARRIS, TB AF ROUBENOFF, R KIEL, DP HANNAN, MT DALLAL, GE WILSON, PWF HARRIS, TB TI VALIDATION OF BIOELECTRICAL-IMPEDANCE (BIA) IN AN AMBULATORY ELDERLY POPULATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 TUFTS UNIV,HUMAN NUTR RES CTR AGING,JMUSDA,BOSTON,MA 02111. HEBREW REHABIL CTR AGED,RES INST,BOSTON,MA 02131. BOSTON UNIV,SCH MED,BOSTON,MA 02118. FRAMINLGHAM HEART STUDY,FRAMINGHAM,MA 01701. NIA,EDBP,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A980 EP A980 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602069 ER PT J AU SCHNAPER, HW KOPP, JB TOBAR, A KLEINMAN, HK AF SCHNAPER, HW KOPP, JB TOBAR, A KLEINMAN, HK TI ASSUMPTION OF A MATRIX-ACCUMULATING PHENOTYPE BY HUMAN GLOMERULAR MESANGIAL CELLS SUBJECTED TO SERIAL PASSAGE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD. NORTHWESTERN UNIV,SCH MED,CHICAGO,IL. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A696 EP A696 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600416 ER PT J AU SEKALY, RP THIBODEAU, J CROTEAU, G LABRECQUE, N ARONSON, HE CANTIN, C LONG, EO FLEURY, S AF SEKALY, RP THIBODEAU, J CROTEAU, G LABRECQUE, N ARONSON, HE CANTIN, C LONG, EO FLEURY, S TI HLA-DR POLYMORPHISM AFFECTS THE INTERACTION WITH CD4 SO FASEB JOURNAL LA English DT Meeting Abstract C1 INST RECH CLIN MONTREAL,IMMUNOL LAB,MONTREAL,PQ,CANADA. UNIV MONTREAL,DEPT MICROBIOL & IMMUNOL,MONTREAL,PQ,CANADA. COLUMBIA UNIV,DEPT BIOCHEM & MOLEC BIOPHYS,NEW YORK,NY 10027. NIAID,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A1055 EP A1055 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602495 ER PT J AU SHANKARAPPA, B NICHOLAS, HB GUPTA, P RINALDO, CR GORRY, MC NARA, PL EHRLICH, GD AF SHANKARAPPA, B NICHOLAS, HB GUPTA, P RINALDO, CR GORRY, MC NARA, PL EHRLICH, GD TI GENOTYPIC CLUSTERING OF THE INFECTING QUASI-SPECIES AND INCREASED VARIABILITY AT LATER TIME POINTS ARE ASSOCIATED WITH A MORE RAPID DECLINE OF CD4+ CELL NUMBERS IN HIV-1 INFECTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK,MD 21701. UNIV PITTSBURGH,PITTSBURGH,PA 15261. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A1052 EP A1052 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602476 ER PT J AU SHEFFLER, LA WINK, DA MELILLO, G COX, GW AF SHEFFLER, LA WINK, DA MELILLO, G COX, GW TI EXOGENOUS NITRIC-OXIDE (NO) REGULATES INTERFERON-GAMMA PLUS LIPOPOLYSACCHARIDE-INDUCED NO SYNTHASE EXPRESSION IN MOUSE MACROPHAGES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,BIOL RESPONSE MODIFIERS PROGRAM,EXPTL IMMUNOL LAB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A1039 EP A1039 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602399 ER PT J AU SHOAF, SE SCHMALL, B AF SHOAF, SE SCHMALL, B TI DEVELOPMENT OF ALPHA-METHYL-L-TRYPTOPHAN (ALPHA-MTP) AS A TRACER OF BRAIN-SEROTONIN SYNTHESIS IN RHESUS-MONKEY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,BETHESDA,MD 20892. NIH,CTR CLIN,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A688 EP A688 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600373 ER PT J AU SILVER, J AKOLKAR, PN GULWANIAKOLKAR, B ROBINSON, MA AF SILVER, J AKOLKAR, PN GULWANIAKOLKAR, B ROBINSON, MA TI INFLUENCE OF NON-HLA GENES ON THE TCR REPERTOIRE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,ROCKVILLE,MD. N SHORE UNIV HOSP,CORNELL UNIV MED COLL,MANHASSET,NY 11030. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A817 EP A817 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601113 ER PT J AU STENGEL, PW RIPPY, MK COCKERHAM, SL DEVANE, WA SILBAUGH, SA AF STENGEL, PW RIPPY, MK COCKERHAM, SL DEVANE, WA SILBAUGH, SA TI ANTIINFLAMMATORY AND BRONCHODILATOR ACTIONS OF ANANDAMIDE, A PUTATIVE CANNABINOID RECEPTOR AGONIST, IN GUINEA-PIGS SO FASEB JOURNAL LA English DT Meeting Abstract C1 ELI LILLY & CO,INDIANAPOLIS,IN 46285. NIMH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A718 EP A718 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600540 ER PT J AU STUBER, E NEURATH, M CALDERHEAD, D FELL, P STROBER, W AF STUBER, E NEURATH, M CALDERHEAD, D FELL, P STROBER, W TI THE OX40-OX40L INTERACTION - A NOVEL PATHWAY IN T-CELL-DEPENDENT B-CELL ACTIVATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,CLIN INVEST LAB,MUCOSAL IMMUN SECT,BETHESDA,MD 20892. BRISTOL MYERS SQUIBB PHARMACEUT RES INST,DEPT MOLEC IMMUNOL,SEATTLE,WA 98121. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A771 EP A771 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600847 ER PT J AU SWIETER, M BERENSTEIN, EH SIRAGANIAN, RP AF SWIETER, M BERENSTEIN, EH SIRAGANIAN, RP TI PROTEIN-TYROSINE-PHOSPHATASE COPRECIPITATES WITH THE HIGH-AFFINITY IGE RECEPTOR AND DEPHOSPHORYLATES THE RECEPTOR SUBUNITS BUT NOT LYN OR SYK SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A781 EP A781 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600903 ER PT J AU TOMASI, M KISSIN, E MCCARTNEYFRANCIS, N WAHL, SM SMITH, PD AF TOMASI, M KISSIN, E MCCARTNEYFRANCIS, N WAHL, SM SMITH, PD TI AGE-RELATED DECLINE IN MACROPHAGE PRODUCTION OF NITRIC-OXIDE (NO) SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV ALABAMA,SCH MED,BIRMINGHAM,AL 35294. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A1039 EP A1039 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602402 ER PT J AU VILLALOBOS, AR PARMELEE, JT PRITCHARD, JB AF VILLALOBOS, AR PARMELEE, JT PRITCHARD, JB TI TETRAETHYLAMMONIUM (TEA) TRANSPORT BY PRIMARY CULTURES OF RAT CHOROID-PLEXUS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. MANCHESTER COMMUNITY TECH COLL,MANCHESTER,CT 06045. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A915 EP A915 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601695 ER PT J AU VISSER, M GALLAGHER, D HARRIS, TB HEYMSFIELD, SB AF VISSER, M GALLAGHER, D HARRIS, TB HEYMSFIELD, SB TI DIFFERENCES IN APPENDICULAR SKELETAL-MUSCLE MASS BETWEEN BLACK-AND-WHITE SUBJECTS SO FASEB JOURNAL LA English DT Meeting Abstract C1 AGR UNIV WAGENINGEN,DEPT HUMAN NUTR,WAGENINGEN,NETHERLANDS. ST LUKES ROOSEVELT HOSP,OBES RES CTR,NEW YORK,NY 10025. NIA,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A980 EP A980 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602066 ER PT J AU WANG, J HARGROVE, ME TING, CC AF WANG, J HARGROVE, ME TING, CC TI DIFFERENTIAL REGULATION BY IL-4 OF PROTEIN-TYROSINE PHOSPHORYLATION IN IL-2-INDUCED LAK CELLS AND ALPHA-CD3-INDUCED CD3-AK CELLS AND THE CORRELATION WITH THEIR CYTOLYTIC ACTIVITY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A823 EP A823 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601153 ER PT J AU WASHBURN, BS TULLIS, K BADEN, DG REIN, K WALSH, PJ HINTON, DE DENISON, MS AF WASHBURN, BS TULLIS, K BADEN, DG REIN, K WALSH, PJ HINTON, DE DENISON, MS TI MECHANISM OF INDUCTION OF CYTOCHROME P4501A BY BREVETOXIN (PBTX), A POLYETHER MARINE NEUROTOXIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CALIF DAVIS,DAVIS,CA 95616. UNIV MIAMI,ROSENSTIEL SCH MARINE & ATMOSPHER SCI,NIEHS,CTR MARINE & FRESHWATER BIOMED SCI,MIAMI,FL 33149. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A714 EP A714 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600517 ER PT J AU WITONSKY, S FOSTER, C BOWEN, J NEILSEN, N SOMMARDAHL, C SIEBENLIST, U WOYCHIK, RP WILKINSON, JE AF WITONSKY, S FOSTER, C BOWEN, J NEILSEN, N SOMMARDAHL, C SIEBENLIST, U WOYCHIK, RP WILKINSON, JE TI PATHOBIOLOGY OF NF-KB TRANSGENIC MICE SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV TENNESSEE,COLL VET MED,KNOXVILLE,TN 37901. OAK RIDGE NATL LAB,DIV BIOL,OAK RIDGE,TN 37831. NIH,BETHESDA,MD 20814. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A721 EP A721 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600558 ER PT J AU ZHU, Q ZHANG, M BLASE, RM DERRY, JMJ CHEN, SH FRANCKE, U OCHS, HD AF ZHU, Q ZHANG, M BLASE, RM DERRY, JMJ CHEN, SH FRANCKE, U OCHS, HD TI MUTATION ANALYSIS OF THE WISKOTT-ALDRICH SYNDROME GENE SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV WASHINGTON,SCH MED,SEATTLE,WA 98195. NIH,BETHESDA,MD 20892. STANFORD UNIV,HOWARD HUGHES MED INST,STANFORD,CA 94305. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A780 EP A780 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600896 ER PT J AU KIM, HY KLAUSNER, RD ROUAULT, TA AF KIM, HY KLAUSNER, RD ROUAULT, TA TI TRANSLATIONAL REPRESSOR ACTIVITY IS EQUIVALENT AND IS QUANTITATIVELY PREDICTED BY IN-VITRO RNA-BINDING FOR 2 IRON-RESPONSIVE ELEMENT-BINDING PROTEINS, IRP1 AND IRP2 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Note ID FERRITIN MESSENGER-RNA; PURIFICATION; REGION AB Iron regulatory proteins (IRPs) bind to specific RNA stem-loop structures known as iron-responsive elements (IREs) which mediate the post-transcriptional regulation of many genes of iron metabolism. Most studies have focused on the role of IRP1, which has previously been shown to bind with high affinity to IREs and mediate repression of in vitro translation of ferritin mRNAs. More recently, a second IRP has been identified that is expressed in all tissues and that binds IREs (Rouault, T. A., Haile, D. H., Downey, W. E., Philpott, C. C., Tang, C., Samaniego, F., Chin, J., Paul, I., Orloff, D., Harford, J. B., and Klausner, R. D. (1992) BioMetals 5, 131-140; Henderson, B. R., Seiser, C., and Kuhn, L. C. (1993) J. Biol. Chem. 268, 27327-27334; Guo, B., Yu, Y., and Leibold, E. A. (1994) J. Biol. Chem. 269, 24252-24260; Samaniego, F., Chin, J., Iwai, K., Rouault, T. A., and Klausner, R. D. (1994) J. Biol. Chem. 269, 30904-30910). Here we report that purified recombinant IRP2 inhibits translation of ferritin mRNAs with a molar efficacy equal to that of recombinant IRP1. There is a quantitative correlation between binding to isolated RNA target motifs, as judged by gel retardation assays, and translational repressor function as assayed in an in vitro translation system. In contrast to IRP1, IRP2 is not inactivated for RNA binding by alkylation with N-ethylmaleimide or phenylmaleimide, and as we would therefore predict, IRP2 treated with N-ethyhmaleimide remains an effective repressor of ferritin translation. As IRP1 and IRP2 clearly have equal capability of mediating translational repression in vitro, the contributions of both IRPs to overall regulation must be considered in describing the pathways of iron regulated gene expression in individual cells. RP KIM, HY (reprint author), NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892, USA. NR 24 TC 74 Z9 73 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 10 PY 1995 VL 270 IS 10 BP 4983 EP 4986 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QL580 UT WOS:A1995QL58000008 PM 7890603 ER PT J AU KOLEY, AP BUTERS, JTM ROBINSON, RC MARKOWITZ, A FRIEDMAN, FK AF KOLEY, AP BUTERS, JTM ROBINSON, RC MARKOWITZ, A FRIEDMAN, FK TI CO BINDING-KINETICS OF HUMAN CYTOCHROME-P450 3A4 - SPECIFIC INTERACTION OF SUBSTRATES WITH KINETICALLY DISTINGUISHABLE CONFORMERS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HUMAN-LIVER; MICROSOMAL CYTOCHROME-P-450; DRUG-METABOLISM; ENZYME; IDENTIFICATION; PURIFICATION; OXIDATION; PROTEIN; ACID AB The kinetics of CO binding to human cytochrome P450 3A4 was examined by the flash photolysis technique, employing the membrane-bound P450 expressed in baculovirus-infected SF9 insect cells, Triexponential kinetics was observed, indicating that P450 3A4 is composed of multiple, kinetically distinguishable conformers. To define the substrate specificity of individual P450 3A4 conformers we evaluated the effect of a series of substrates of varying sizes and structures on the CO binding kinetics. The rate of CO binding to the total mixture of P450 3A4 conformers was increased in the presence of nifedipine and erythromycin, decreased by quinidine, testosterone, and warfarin, and unaffected by cimetidine and 17 alpha-ethynylestradiol. A recently developed kinetic difference method (Koley, A. P., Robinson, R. C., Markowitz, A., and Friedman, F. K. (1994) Biochemistry 33, 2484-2489) was used to define the kinetic parameters of individual P450 3A4 conformers. The results showed that different conformers have distinct substrate specificities. The substrates had markedly variable effects on the CO binding kinetics of their target P450 3A4 conformers and thus differentially modulate their conformations. These results demonstrate that the interaction of a particular substrate with a specific P450 3A4 conformer can be assessed in the presence of multiple conformers. C1 NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. NIH,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. RI Buters, Jeroen/G-5070-2011; Friedman, Fred/D-4208-2016 NR 24 TC 83 Z9 83 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 10 PY 1995 VL 270 IS 10 BP 5014 EP 5018 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QL580 UT WOS:A1995QL58000013 PM 7890608 ER PT J AU BEITNERJOHNSON, D LEROITH, D AF BEITNERJOHNSON, D LEROITH, D TI INSULIN-LIKE GROWTH-FACTOR-I STIMULATES TYROSINE PHOSPHORYLATION OF ENDOGENOUS C-CRK SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PHOSPHATIDYLINOSITOL 3'-KINASE; RECEPTOR SUBSTRATE-1; PROTEINS; CELLS; IRS-1; SH2; ASSOCIATION; BINDING; GENE; GRB2 AB Crk, a cellular homolog of v-crk, is an SH2 and SH3 domain-containing adaptor protein related to Grb2 and Nck, two proteins which have been shown to be involved in growth factor signal transduction. Crk proteins have recently been found to associate with two guanine nucleotide releasing proteins, mSos and C3G, and thus appear to lie on the Ras pathway. We investigated whether Crk is a target for the insulin-like growth factor I (IGF-I) receptor tyrosine kinase. We show that IGF-I stimulates tyrosine phosphorylation of Crk II via stimulation of endogenous IGF-I receptors in both 293 cells and NIH-3T3 cells. IGF-I stimulated tyrosine phosphorylation of Crk II in a dose- and time-dependent manner. In 293 cells, which express both IGF-I and insulin receptors, insulin also induced a dose dependent tyrosine phosphorylation of Crk II, but with somewhat reduced sensitivity, compared to IGF-I. In NIH 3T3 cells, IGF-I also stimulated tyrosine phosphorylation of a 45- kDa protein which co immunoprecipitated with Crk II. These findings indicate that Crk II is an endogenous substrate of the IGF-I receptor tyrosine kinase and provide the first demonstration that a mitogenic growth factor induces tyrosine phosphorylation of endogenous c-Crk. RP BEITNERJOHNSON, D (reprint author), NIDDK,DIABET BRANCH,BLDG 10,RM 8S-239,10 CTR DR,MSC 1770,BETHESDA,MD 20892, USA. NR 33 TC 99 Z9 100 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 10 PY 1995 VL 270 IS 10 BP 5187 EP 5190 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QL580 UT WOS:A1995QL58000037 PM 7534289 ER PT J AU SCHMIDT, HHJ REMALEY, AT STONIK, JA RONAN, R WELLMANN, A THOMAS, F ZECH, LA BREWER, HB HOEG, JM AF SCHMIDT, HHJ REMALEY, AT STONIK, JA RONAN, R WELLMANN, A THOMAS, F ZECH, LA BREWER, HB HOEG, JM TI CARBOXYL-TERMINAL DOMAIN TRUNCATION ALTERS APOLIPOPROTEIN-A-I IN-VIVO CATABOLISM SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HIGH-DENSITY-LIPOPROTEINS; LECITHIN-CHOLESTEROL ACYLTRANSFERASE; CORONARY HEART-DISEASE; INVIVO METABOLISM; EXCHANGEABLE APOLIPOPROTEINS; AMPHIPATHIC HELIX; APOA-I; PLASMA; DEGRADATION; CONVERSION AB Apolipoprotein A-I (apoA-I), the major protein of high density lipoproteins, facilitates reverse cholesterol transport from peripheral tissue to liver. To determine the structural motifs important for modulating the in vivo catabolism of human apoA-I (h-apoA-I), we generated carboxyl-terminal truncation mutants at residues 201 (apoA-I-201), 217 (apoA-I-217), and 226 (apoA-I-226) by site directed mutagenesis. ApoA-I was expressed in Escherichia coli as a fusion protein with the maltose binding protein, which was removed by factor Xa cleavage. The in vivo kinetic analysis of the radioiodinated apoA-I in normolipemic rabbits revealed a markedly increased rate of catabolism for the truncated forms of apoA-I. The fractional catabolic rates (FCR) of 9.10 +/- 1.28/day (+/-S.D.) for apoA-I-201, 6.34 +/- 0.81/day for apoA-I-217, and 4.42 +/- 0.51/day for apoA-I-226 were much faster than the FCR of recombinant intact apoA-I (r-apoA-I, 0.93 +/- 0.07/day) and h-apoA-I (0.91 +/- 0.34/day), All the truncated forms of apoA-I were associated with very high density lipoproteins, whereas the intact recombinant apoA-I (r-apoA-I) and h-apoA-I associated with HDL(2) and HDL(3), Gel filtration chromatography revealed that in contrast to r-apoA-I, the mutant apoA-I-201 associated with a phospholipid-rich rabbit apoA-I containing particle. Analysis by agarose gel electrophoresis demonstrated that the same mutant migrated in the pre-beta position, but not within the alpha position as did r-apoA-I. These results indicate that the carboxyl-terminal region (residue 227-243) of apoA-I is critical in modulating the association of apoA-I with lipoproteins and in vivo metabolism of apoA-I. C1 NHLBI, CELL BIOL SECT, MOLEC DIS BRANCH, BETHESDA, MD 20892 USA. NR 75 TC 60 Z9 61 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 10 PY 1995 VL 270 IS 10 BP 5469 EP 5475 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QL580 UT WOS:A1995QL58000079 PM 7890663 ER PT J AU WHITEHURST, CE OWAKI, H BRUDER, JT RAPP, UR GEPPERT, TD AF WHITEHURST, CE OWAKI, H BRUDER, JT RAPP, UR GEPPERT, TD TI THE MEK KINASE-ACTIVITY OF THE CATALYTIC DOMAIN OF RAF-1 IS REGULATED INDEPENDENTLY OF RAS BINDING IN T-CELLS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ACTIVATED PROTEIN-KINASE; MAP KINASE; SIGNAL TRANSDUCTION; THREONINE KINASE; TYROSINE PHOSPHORYLATION; SERINE PHOSPHORYLATION; TRANSCRIPTION FACTOR; BIOLOGICAL-ACTIVITY; RECEPTOR; INHIBITION AB Deletion of the amino-terminal domain of Raf-1, which contains the Ras-binding region, results in the constitutive activation of the liberated Raf-1 catalytic domain in fibroblast cell lines. We demonstrate that the MEK kinase activity of the isolated Raf-1 catalytic domain, Raf-BXB, is not constitutively active, but is regulated in Jurkat T cells. Raf-BXB is activated by engaging the antigen receptor-CD3 complex, or treating cells with phorbol myristate acetate or okadaic acid. Increasing intracellular cAMP inhibits Raf-1 activation stimulated by phorbol myristate acetate, but not the activation of Raf-BXB. Serine 621, but not serine 499, is essential for Raf-BXB MEK kinase activity. Because Raf-BXB does not bind Ras, the data establishes a Ras-independent signal in directly regulating the activity of the Raf-1 catalytic domain. C1 UNIV TEXAS,SW MED CTR,DEPT INTERNAL MED,DALLAS,TX 75235. UNIV TEXAS,SW MED CTR,GRAD PROGRAM IMMUNOL,DALLAS,TX 75235. NCI,FREDERICK CANC RES & DEV CTR,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702. NR 65 TC 53 Z9 53 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 10 PY 1995 VL 270 IS 10 BP 5594 EP 5599 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QL580 UT WOS:A1995QL58000096 PM 7534298 ER PT J AU COSO, OA CHIARIELLO, R KALINEC, G KYRIAKIS, JM WOODGETT, J GUTKIND, JS AF COSO, OA CHIARIELLO, R KALINEC, G KYRIAKIS, JM WOODGETT, J GUTKIND, JS TI TRANSFORMING G-PROTEIN-COUPLED RECEPTORS POTENTLY ACTIVATE JNK (SAPK) - EVIDENCE FOR A DIVERGENCE FROM THE TYROSINE KINASE SIGNALING PATHWAY SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID GENES; JUN; PHOSPHORYLATION; TRANSDUCTION; RAS AB The expression of human muscarinic acetylcholine receptors (mAChRs) in NIH 3T3 cells has been used as a model for studying proliferative signaling through G protein-coupled receptors. In this biological system, the mi class of mAChRs can effectively transduce mitogenic signals (Stephens, E. V., Kalinec, G., Brann, M. R., and Gutkind, J. S. (1993) Oncogene 8, 19-26) and induce malignant transformation if persistently activated (Gutkind, J. S., Novotny, E. A., Brann, M. R., and Robbins, K. C. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 4703-4708). Moreover, available evidence suggests that the mi-signaling pathway converges at the level of p21(ras) with that emerging from tyrosine kinase receptors (Crespo, P., Xu, N., Simonds, W. F., and Gutkind, J. S. (1994) Nature 369, 418-420). To explore nuclear events involved in growth regulation by G protein-coupled receptors in this setting, we compared the effect of platelet-derived growth factor (PDGF) and the cholinerse agonist, carbachol, on the expression of mRNA for members of the jun and fos family of nuclear proto-oncogenes. We found that activation of m1 receptors by carbachol induces the expression of a distinct set of nuclear transcription factors. In particular, carbachol caused a much greater induction of c-jun mRNA and AP-1 activity. These responses did not correlate with protein kinase C stimulation nor with the activation of mitogen-activated protein (MAP) kinases. Recently, it has been shown that a novel family of kinases structurally related to MAP kinases, stress-activated protein kinases, or Jun kinases (JNKs), phospho rylate in vivo the amino-terminal transactivating domain of the c-Jun protein, thereby increasing its transcriptional activity. In view of our results, this observation prompted us to ask whether m1 and PDGF can differentially activate JNKs. Here, we show that m1 mAChRs can induce a remarkable increase in JNK activity, which was temporally distinct from that of MAP kinase and was entirely protein kinase C independent. In contrast, PDGF failed to activate JNK in these cells, although it stimulated MAP kinase to an extent even greater than that for carbachol. These findings demonstrate that G protein-coupled receptors can signal through pathways leading to the activation of JNK, thus diverging at this level with those signaling routes utilized by tyrosine kinase receptors. C1 NIDR,CELLULAR DEV & ONCOL LAB,MOLEC SIGNALING UNIT,BETHESDA,MD 20892. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DIABET UNIT,BOSTON,MA 02129. PRINCESS MARGARET HOSP,ONTARIO CANC INST,TORONTO,ON M4X 1K9,CANADA. RI Gutkind, J. Silvio/A-1053-2009; Woodgett, Jim/F-1087-2010; Chiariello, Mario/O-3642-2014 OI Woodgett, Jim/0000-0003-3731-5797; Chiariello, Mario/0000-0001-8434-5177 NR 28 TC 211 Z9 212 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 10 PY 1995 VL 270 IS 10 BP 5620 EP 5624 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QL580 UT WOS:A1995QL58000100 PM 7890682 ER PT J AU CASSELL, DJ SCHWARTZ, RH AF CASSELL, DJ SCHWARTZ, RH TI A COMPARISON OF B-CELL AND DENDRITIC CELL ANTIGEN PRESENTATION TO NAIVE T-CELLS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,CELLULAR & MOLEC IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 4 EP 4 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400007 ER PT J AU GERMAIN, RN CASTELLINO, F ROMAGNOLI, P MADRENAS, J WANGE, R ISAKOV, N SAMELSON, LE AF GERMAIN, RN CASTELLINO, F ROMAGNOLI, P MADRENAS, J WANGE, R ISAKOV, N SAMELSON, LE TI FORMATION AND FUNCTION OF PEPTIDE - MHC MOLECULE LIGANDS FOR THE TCR SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,LYMPHOCYTE BIOL SECT,BETHESDA,MD 20892. NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892. RI Romagnoli, Paola/K-2237-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 4 EP 4 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400006 ER PT J AU WEISSMAN, D ANANWORANICH, J BARKER, TD DAUCHER, JA LI, YX ORENSTEIN, JM FAUCI, AS AF WEISSMAN, D ANANWORANICH, J BARKER, TD DAUCHER, JA LI, YX ORENSTEIN, JM FAUCI, AS TI ROLE OF DENDRITIC CELLS IN THE IMMUNOPATHOGENESIS OF HIV DISEASE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. GEORGE WASHINGTON UNIV,MED CTR,WASHINGTON,DC 20037. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 8 EP 8 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400018 ER PT J AU JANIK, JE MILLER, LL KOPP, W GAUSE, B CURTI, B LONGO, DL AF JANIK, JE MILLER, LL KOPP, W GAUSE, B CURTI, B LONGO, DL TI A PHASE-I TRIAL OF TUMOR-NECROSIS-FACTOR (TNF) AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) TO ENHANCE DENDRITIC CELL MATURATION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 11 EP 11 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400032 ER PT J AU SCHWARZENBERGER, K UDEY, MC AF SCHWARZENBERGER, K UDEY, MC TI MODULATION OF LANGERHANS CELL E-CADHERIN EXPRESSION DURING THE INITIATION PHASE OF CONTACT SENSITIVITY REACTIONS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,DERMATOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 14 EP 14 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400042 ER PT J AU MAURER, D EBNER, C REININGER, B FIEBIGER, E KRAFT, D KINET, JP STINGL, G AF MAURER, D EBNER, C REININGER, B FIEBIGER, E KRAFT, D KINET, JP STINGL, G TI FC-EPSILON-RI MEDIATES IGE-DEPENDENT ALLERGEN PRESENTATION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UNIV VIENNA,SCH MED,DIAID,A-1010 VIENNA,AUSTRIA. NIAID,MOL ALL & IMMUNOL SECT,ROCKVILLE,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 21 EP 21 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400067 ER PT J AU RIDGE, JP FUCHS, E MATZINGER, P AF RIDGE, JP FUCHS, E MATZINGER, P TI NEONATAL TOLERANCE IS A NUMBERS GAME SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,CELLULAR & MOLEC IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 21 EP 21 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400068 ER PT J AU LONG, EO BAKKE, O PINET, V AF LONG, EO BAKKE, O PINET, V TI ANTIGEN PRESENTATION MEDIATED BY RECYCLING OF SURFACE HLA-DR MOLECULES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOGENT LAB,ROCKVILLE,MD 20852. UNIV OSLO,DEPT BIOL,OSLO 3,NORWAY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 28 EP 28 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400096 ER PT J AU RIVERO, JL IENDOUBI, M AF RIVERO, JL IENDOUBI, M TI INVARIANT CHAIN KNOCKOUT MICE A TOOL TO STUDY AUTOIMMUNE-DISEASES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NEI,GENET & IMMUNOL SECT,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 31 EP 31 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400106 ER PT J AU BLAUVELT, A CHOUGNET, C SHEARER, GM KATZ, SI AF BLAUVELT, A CHOUGNET, C SHEARER, GM KATZ, SI TI PROTEIN ANTIGEN PRESENTATION BY EPIDERMAL LANGERHANS CELLS IN NORMAL-APPEARING SKIN OF INDIVIDUALS WITH AIDS IS NORMAL SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,DERMATOL BRANCH,BETHESDA,MD 20892. NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 33 EP 33 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400114 ER PT J AU ESPEY, MG TANG, Y MORSE, HC MOFFETT, JR ARYAN, MA AF ESPEY, MG TANG, Y MORSE, HC MOFFETT, JR ARYAN, MA TI DENDRITIC CELLS, QUINOLINIC ACID, AND THE MURINE MODEL OF AIDS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 GEORGETOWN UNIV,DEPT BIOL,WASHINGTON,DC 20057. NIAID,IMMUNOPATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 33 EP 33 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400117 ER PT J AU MANN, DL KASLOW, RA APPLE, R CARRINGTON, M MUNOZ, A PARK, L DETELS, R RINALDO, C PHAIR, J GEODERT, J SAAH, A AF MANN, DL KASLOW, RA APPLE, R CARRINGTON, M MUNOZ, A PARK, L DETELS, R RINALDO, C PHAIR, J GEODERT, J SAAH, A TI HLA CLASS-I, CLASS-II, AND TAP GENES STRONGLY INFLUENCE THE COURSE OF HIV-INFECTION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,FREDERICK,MD 21702. RI apple, raymond/I-4506-2012 OI apple, raymond/0000-0002-8007-0345 NR 0 TC 0 Z9 0 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 34 EP 34 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400119 ER PT J AU SHER, A AF SHER, A TI LESSONS FROM PARASITES ON THE INITIATION AND REGULATION OF CELLULAR IMMUNE FUNCTION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,PARASIT DIS LAB,IMMUNOBIOL SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 56 EP 56 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400180 ER PT J AU CLERICI, M SARIN, A LUCEY, DR PINTO, LA WYNN, TA BLATT, SP HENDRIX, CW DOLAN, MJ WOLF, SF BERZOFSKY, JA HENKART, PA SHEARER, GM AF CLERICI, M SARIN, A LUCEY, DR PINTO, LA WYNN, TA BLATT, SP HENDRIX, CW DOLAN, MJ WOLF, SF BERZOFSKY, JA HENKART, PA SHEARER, GM TI MODIFICATION OF THE IMMUNE-RESPONSE IN HIV-INFECTION BY TYPE-1 TYPE-2 CYTOKINE REGULATION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 GENET INST INC,CAMBRIDGE,MA. UNIV MILAN,I-20122 MILAN,ITALY. NCI,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. WILFORD HALL USAF MED CTR,LACKLAND AFB,TX 78236. RI Wynn, Thomas/C-2797-2011; Hendrix, Craig/G-4182-2014 OI Hendrix, Craig/0000-0002-5696-8665 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 60 EP 60 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400193 ER PT J AU COMBADIERE, B FREEDMAN, M LENARDO, MJ AF COMBADIERE, B FREEDMAN, M LENARDO, MJ TI DISTINCT T-CELL RECEPTOR SIGNALING PATHWAYS CONTROL LYMPHOKINE INDUCTION AND PROGRAMMED CELL-DEATH IN MATURE T-LYMPHOCYTES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. RI Combadiere, Behazine/G-3881-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 66 EP 66 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400214 ER PT J AU KIM, YH BUCHHOLZ, MA NORDIN, AA AF KIM, YH BUCHHOLZ, MA NORDIN, AA TI A NEW ASPECT OF IL-2 R SIGNALING PATHWAY IN MURINE T-LYMPHOCYTES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 KYUNGPOOK NATL UNIV,COLL NAT SCI,DEPT MICROBIOL,TAEGU 702701,SOUTH KOREA. NIA,GERONTOL RES CTR,CLIN IMMUNOL SECT,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 74 EP 74 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400246 ER PT J AU LENCZOWSKI, JM ZACHARCHUK, CM BIRRER, M ASHWELL, JD AF LENCZOWSKI, JM ZACHARCHUK, CM BIRRER, M ASHWELL, JD TI THE EXPRESSION OF A DOMINANT-NEGATIVE CJUN ALTERS INDUCTION OF AP-1 COMPONENTS IN T-CELL LINES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,LICB,BETHESDA,MD 20892. NIH,HHMI,RES SCHOLARS PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 76 EP 76 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400252 ER PT J AU KENNY, JJ FISCHER, RT REED, JC LONGO, DL AF KENNY, JJ FISCHER, RT REED, JC LONGO, DL TI BCL-2 PREVENTS THE CLONAL DELETION OF PHOSPHOCHOLINE-SPECIFIC B-CELLS IN MU-KAPPA BUT NOT IN MU-ONLY M167 TRANSGENIC XID MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 LA JOLLA CANC RES FDN,LA JOLLA,CA 92037. NCI,FCRDC,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 98 EP 98 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400342 ER PT J AU LOBANOFF, MC LAI, JC FUKUSHIMA, A WAWROUSEK, EF LEE, RS WHITCUP, SM SMITHGILL, SJ AF LOBANOFF, MC LAI, JC FUKUSHIMA, A WAWROUSEK, EF LEE, RS WHITCUP, SM SMITHGILL, SJ TI IMMUNOTOLERANCE IN TRANSGENIC MICE EXPRESSING A FOREIGN ANTIGEN IN A SEQUESTERED ORGAN SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NEI,BETHESDA,MD 20892. HOWARD HUGHES MED INST,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 101 EP 101 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400352 ER PT J AU LONG, EO BAKKE, O PINET, V AF LONG, EO BAKKE, O PINET, V TI ANTIGEN PRESENTATION MEDIATED BY RECYCLING OF SURFACE HLA-DR MOLECULES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOGENET LAB,BETHESDA,MD 20892. UNIV OSLO,DEPT BIOL,OSLO 3,NORWAY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 101 EP 101 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400354 ER PT J AU BENDELAC, A AF BENDELAC, A TI THE LIGAND OF MOUSE NK1.1+T-CELLS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. PRINCETON UNIV,PRINCETON,NJ 08544. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 112 EP 112 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400396 ER PT J AU FISHER, GH ZUNIGAPFLUCKER, JC LENARDO, M AF FISHER, GH ZUNIGAPFLUCKER, JC LENARDO, M TI TCR CROSS-LINKING IS SUFFICIENT FOR ACTIVATION BUT NOT DELETION OF DOUBLE-POSITIVE THYMOCYTES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20814. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 117 EP 117 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400417 ER PT J AU GIESE, T DAVIDSON, WF AF GIESE, T DAVIDSON, WF TI CD8+ T-CELLS ARE THE PREDOMINANT SOURCE OF B220+ DOUBLE-NEGATIVE T-CELLS IN LPR AND GLD MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,GENET LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 118 EP 118 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400421 ER PT J AU KHATTRI, R QIAN, DP LOVE, PE FITCH, FW BLUESTONE, JA AF KHATTRI, R QIAN, DP LOVE, PE FITCH, FW BLUESTONE, JA TI T-CELL RECEPTOR GAMMA-DELTA-CELL DEVELOPMENT AND FUNCTION IN ZETA-DEFICIENT MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UNIV CHICAGO,BEN MAY INST,CHICAGO,IL 60637. UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143. NICHHD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 122 EP 122 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400434 ER PT J AU SOMMERS, CL HUANG, K GRINBERG, A LOVE, PE AF SOMMERS, CL HUANG, K GRINBERG, A LOVE, PE TI CLONING OF MURINE TXK - A PROTEIN-TYROSINE KINASE EXPRESSED EARLY IN FETAL THYMIC DEVELOPMENT SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NICHHD,MAMMALIAN GENES & DEV LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 130 EP 130 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400466 ER PT J AU VACCHIO, MS ASHWELL, JD AF VACCHIO, MS ASHWELL, JD TI INFLUENCE OF THYMIC-DERIVED STEROIDS ON POSITIVE SELECTION OF THYMOCYTES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 US FDA,CBER,DIV HEMATOL PROD,BETHESDA,MD 20892. NCI,IMMUNE CELL BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 132 EP 132 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400473 ER PT J AU ALEXANDERMILLER, MA PARKER, KC TSUKUI, T PENDLETON, D COLIGAN, JE BERZOFSKY, JA AF ALEXANDERMILLER, MA PARKER, KC TSUKUI, T PENDLETON, D COLIGAN, JE BERZOFSKY, JA TI P18-SPECIFIC, HLA-A2 RESTRICTED AND H-2D(I) RESTRICTED CTL SHARE A COMMON MINIMAL EPITOPE WHICH UTILIZED SIMILAR RESIDUES FOR MHC BINDING SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,METAB BRANCH,BETHESDA,MD 20892. NIAID,MOLEC STRUCT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 135 EP 135 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400485 ER PT J AU BERZOFSKY, JA TAKESHITA, T TAKAHASHI, H KOZLOWSKI, S AHLERS, JD PENDLETON, CD MOORE, RL NAKAGAWA, Y YOKOMURO, K FOX, BS MARGULIES, DH AF BERZOFSKY, JA TAKESHITA, T TAKAHASHI, H KOZLOWSKI, S AHLERS, JD PENDLETON, CD MOORE, RL NAKAGAWA, Y YOKOMURO, K FOX, BS MARGULIES, DH TI MOLECULAR-BASIS OF FUNCTIONAL BINDING OF THE SAME HIV PEPTIDE TO BOTH A CLASS-I AND A CLASS-II MHC MOLECULE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. NCI,METAB BRANCH,BETHESDA,MD 20892. UNIV MARYLAND,SCH MED,DEPT MED,BALTIMORE,MD 21201. NIPPON MED COLL,DEPT MICROBIOL & IMMUNOL,TOKYO 113,JAPAN. RI Margulies, David/H-7089-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 135 EP 135 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400488 ER PT J AU CLERICI, M PINTO, L SHEARER, GM AF CLERICI, M PINTO, L SHEARER, GM TI EVIDENCE FOR IMMUNE PROTECTION TO HIV SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UNIV MILAN,CATTEDRA IMMUNOL,I-20133 MILAN,ITALY. NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 138 EP 138 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400498 ER PT J AU DAVIDSON, WF GIESE, T AF DAVIDSON, WF GIESE, T TI EVIDENCE FOR AGE-RELATED ABNORMALITIES IN THE DELETION OF ACTIVATED PERIPHERAL T-CELLS IN IPR AND GLD MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,GENET LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 139 EP 139 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400501 ER PT J AU DOHERTY, TM GIESE, N MORSE, HC COFFMAN, RL AF DOHERTY, TM GIESE, N MORSE, HC COFFMAN, RL TI THE ROLE OF CYTOKINES IN A MURINE MODEL OF ACQUIRED IMMUNOLOGICAL-UNRESPONSIVENESS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 DNAX INC,CELLULAR & MOLEC BIOL RES INST,PALO ALTO,CA 94304. NIAID,IMMUNOPATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 140 EP 140 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400505 ER PT J AU GUO, WX BURGER, A FISCHER, RT LONGO, DL KENNY, JJ AF GUO, WX BURGER, A FISCHER, RT LONGO, DL KENNY, JJ TI ANALYSIS OF T15-IDIOTYPE NEGATIVE ANTI-PHOSPHOCHOLINE ANTIBODIES FROM X-LINKED IMMUNE-DEFICIENT XID, MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,FCRDC,PRI DYNCORP,FREDERICK,MD 21702. BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 142 EP 142 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400515 ER PT J AU MARSHALL, MA ACTOR, JK SHER, A BERZOFSKY, JA AF MARSHALL, MA ACTOR, JK SHER, A BERZOFSKY, JA TI SUPPRESSION OF HIV-SPECIFIC CTL RESPONSE BY LYMPHOCYTES FROM HELMINTH-INFECTED MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,METAB BRANCH,BETHESDA,MD 20892. NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 148 EP 148 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400537 ER PT J AU MORAWETZ, R GIESE, N MORSE, HC AF MORAWETZ, R GIESE, N MORSE, HC TI RELATIONSHIPS OF CYTOKINE EXPRESSION AND CELL SIGNALING TO PATHOGENESIS OF MAIDS, A RETROVIRUS-INDUCED IMMUNODEFICIENCY SYNDROME OF MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOPATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 149 EP 149 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400543 ER PT J AU CIERNIK, IF YANUCK, M BERZOFSKY, JA CARBONE, DP AF CIERNIK, IF YANUCK, M BERZOFSKY, JA CARBONE, DP TI EXPRESSION OF A MUTANT P53 EPITOPE FUSED WITH THE ADENOVIRUS E3 LEADER SEQUENCE IN TUMOR-CELLS OVERCOMES GAMMA-IFN DEPENDENCE OF LYSIS BY P53-SPECIFIC CTL SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SW MED SCH,SIMMONS CANC CTR,DALLAS,TX 75235. NCI,METAB BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 163 EP 163 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400596 ER PT J AU HATHCOCK, KS PUCILLO, CEM LASZLO, G LAI, L HODES, RJ AF HATHCOCK, KS PUCILLO, CEM LASZLO, G LAI, L HODES, RJ TI ANALYSIS OF NOVEL THYMIC SUBPOPULATIONS EXPRESSING THE CELL-SURFACE MOLECULE GL7 - EXPRESSION, GENETICS, AND FUNCTION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,EIB,BETHESDA,MD 20892. NIA,BETHESDA,MD 20892. PHARMINGEN,SAN DIEGO,CA 92121. RI Pucillo, Carlo/A-5515-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 168 EP 168 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400616 ER PT J AU STEVENSON, MM TAM, MF SHER, A AF STEVENSON, MM TAM, MF SHER, A TI INTERLEUKIN-12 INDUCES A PROTECTIVE TH1 RESPONSE IN PLASMODIUM-CHABAUDI AS SUSCEPTIBLE A/J MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 MONTREAL GEN HOSP,RES INST,CTR STUDY HOST RESISTANCE,MONTREAL,PQ H3G 1A4,CANADA. NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 178 EP 178 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400655 ER PT J AU SADEGHNASSERI, S MARGULIES, D STERN, L GREEN, D AF SADEGHNASSERI, S MARGULIES, D STERN, L GREEN, D TI FORMATION OF SPECIFIC LOW-AFFINITY PEPTIDE CLASS-II DOES NOT REQUIRE PEPTIDE SIDE-CHAIN INTERACTIONS WITH CLASS-II GROOVE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 AMER RED CROSS,DEPT IMMUNOL,ROCKVILLE,MD 20855. NIAID,BETHESDA,MD 20892. MIT,DEPT CHEM,CAMBRIDGE,MA 02139. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 181 EP 181 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400668 ER PT J AU WYNN, TA JANKOVIC, D POINDEXTER, R CASPAR, P CHEEVER, A SHER, A AF WYNN, TA JANKOVIC, D POINDEXTER, R CASPAR, P CHEEVER, A SHER, A TI VACCINATION WITH PARASITE (EGG) ANTIGEN PLUS IL-12 SWITCHES HELMINTH-INDUCED CYTOKINE RESPONSES FROM A TH2 TO A TH1 PATTERN AND BLOCKS PATHOLOGY SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,PARASIT DIS LAB,BETHESDA,MD 20892. RI Wynn, Thomas/C-2797-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 182 EP 182 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400673 ER PT J AU BIESSMANN, H MASON, JM AF BIESSMANN, H MASON, JM TI DNA ORGANIZATION OF DIPTERAN TELOMERES AND THEIR ELONGATION BY SPECIFIC RETROTRANSPOSONS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UNIV CALIF IRVINE,CTR DEV BIOL,IRVINE,CA 92717. NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 186 EP 186 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400679 ER PT J AU VERNICK, K AF VERNICK, K TI MOLECULAR ASPECTS OF PLASMODIUM RESISTANCE IN ANOPHELES-GAMBIAE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 189 EP 189 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400689 ER PT J AU BARREAU, C TOURAY, M MILLER, L VERNICK, K AF BARREAU, C TOURAY, M MILLER, L VERNICK, K TI IDENTIFICATION OF SURFACE MOLECULES OF MOSQUITO SALIVARY-GLANDS WHICH MALARIA SPOROZOITES USE AS RECEPTORS FOR INVASION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 205 EP 205 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400741 ER PT J AU SARAIVA, EMB MIALHE, EL SHAHABUDDIN, M VERNICK, K MILLER, LH AF SARAIVA, EMB MIALHE, EL SHAHABUDDIN, M VERNICK, K MILLER, LH TI RESEARCH FOR GENETIC-TRANSFORMATION OF DIPTERA VECTORS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,MALARIA RES LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 226 EP 226 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400825 ER PT J AU STORZ, G ALTUVIA, S TOLEDANO, MB KULLIK, I AF STORZ, G ALTUVIA, S TOLEDANO, MB KULLIK, I TI THE OXYR REGULON SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 236 EP 236 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400851 ER PT J AU TOLEDANO, MB KULLIK, I STORZ, G AF TOLEDANO, MB KULLIK, I STORZ, G TI REDOX-DEPENDENT SHIFT OF OXYR-DNA CONTACTS ALONG AN EXTENDED DNA-BINDING SITE - A MECHANISM FOR DIFFERENTIAL PROMOTER SELECTION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UMDNJ,RUTGERS COLL PHARM,DEPT PHARMACOL & TOXICOL,PISCATAWAY,NJ 08855. NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 3 U2 6 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 247 EP 247 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400888 ER PT J AU YAMAGUCHIIWAI, Y YUAN, DS DANCIS, A KLAUSNER, RD AF YAMAGUCHIIWAI, Y YUAN, DS DANCIS, A KLAUSNER, RD TI AFT1 - ACTIVATOR OF FERROUS TRANSPORT REGULATES IRON UPTAKE TRANSCRIPTIONALLY IN SACCHAROMYCES-CEREVISIAE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 251 EP 251 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400905 ER PT J AU SUNDARESAN, M YU, ZX IRANI, K FINKEL, T AF SUNDARESAN, M YU, ZX IRANI, K FINKEL, T TI THE ROLE OF HYDROGEN-PEROXIDE IN GROWTH-FACTOR SIGNALING SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 256 EP 256 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400924 ER PT J AU WOLFFE, AP ALMOUZNI, G BOUVET, P DIMITROV, S HAYES, JJ LANDSBERGER, N NIGHTINGLAE, K PRUSS, D URA, K AF WOLFFE, AP ALMOUZNI, G BOUVET, P DIMITROV, S HAYES, JJ LANDSBERGER, N NIGHTINGLAE, K PRUSS, D URA, K TI CHROMATIN STRUCTURE AND GENE-EXPRESSION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract ID TRANSCRIPTION; ACETYLATION C1 NICHHD,MOLEC EMBRYOL LAB,BETHESDA,MD 20892. RI dimitrov, stefan/M-7697-2013 NR 10 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 270 EP 270 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400963 ER PT J AU KUNKEL, TA THOMAS, DC BOYER, JC MINNICK, DT IZUTA, S ROBERTS, JD UMAR, A YIN, S NGUYEN, DC AF KUNKEL, TA THOMAS, DC BOYER, JC MINNICK, DT IZUTA, S ROBERTS, JD UMAR, A YIN, S NGUYEN, DC TI STUDIES OF DNA-REPLICATION FIDELITY IN HUMAN CELL-EXTRACTS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. NR 13 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 271 EP 271 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400964 ER PT J AU WANG, XW EGLY, JM WANG, Z FRIEDBERG, EC EVANS, MK BOHR, VA HOEIJMAKERS, JHJ HARRIS, CC AF WANG, XW EGLY, JM WANG, Z FRIEDBERG, EC EVANS, MK BOHR, VA HOEIJMAKERS, JHJ HARRIS, CC TI P53 DNA-REPAIR AND CARCINOGENESIS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892. FAC MED STRASBOURG,INSERM,CNRS,UPR 6520,F-67000 STRASBOURG,FRANCE. NIA,MOLEC GENET LAB,BALTIMORE,MD 21224. UNIV TEXAS SW MED CTR,DEPT PATHOL,DALLAS,TX 75235. ERASMUS UNIV ROTTERDAM,CTR MED GENET,DEPT CELL BIOL & GENET,3000 DR ROTTERDAM,NETHERLANDS. RI Wang, Xin/B-6162-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 272 EP 272 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400969 ER PT J AU SANDER, M HUANG, SM GU, LY AF SANDER, M HUANG, SM GU, LY TI SINGLE AMINO-ACID CHANGES ALTER THE REPAIR SPECIFICITY OF DROSOPHILA RRP1 - ISOLATION OF MUTANTS DEFICIENT IN REPAIR OF OXIDATIVE DAMAGE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 276 EP 276 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86400982 ER PT J AU WANG, XW YEH, H SCHAEFFER, L ROY, R MONCOLLIN, V EGLY, JM WANG, Z FRIEDBERG, EC EVANS, MK TAFFE, BG BOHR, VA WEEDA, G HOEIJMAKERS, JHJ FORRESTER, K HARRIS, CC AF WANG, XW YEH, H SCHAEFFER, L ROY, R MONCOLLIN, V EGLY, JM WANG, Z FRIEDBERG, EC EVANS, MK TAFFE, BG BOHR, VA WEEDA, G HOEIJMAKERS, JHJ FORRESTER, K HARRIS, CC TI P53 MODULATION OF BTF2-TFIIH ASSOCIATED NUCLEOTIDE EXCISION-REPAIR ACTIVITY SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892. FAC MED STRASBOURG,INSERM,UNITE 184,CNRS,UPR 6520,F-67085 STRASBOURG,FRANCE. NIA,MOLEC GENET LAB,BALTIMORE,MD 21224. ERASMUS UNIV ROTTERDAM,CTR MED GENET,DEPT CELL BIOL & GENET,3000 DR ROTTERDAM,NETHERLANDS. UNIV TEXAS,SW MED CTR,DEPT PATHOL,MOLEC PATHOL LAB,DALLAS,TX 75235. RI Wang, Xin/B-6162-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 281 EP 281 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401000 ER PT J AU OTRIN, VR TAKAO, M MCLENIGAN, M LEVINE, AS PROTIC, M AF OTRIN, VR TAKAO, M MCLENIGAN, M LEVINE, AS PROTIC, M TI EXPRESSION AND REGULATION OF THE UV-DAMAGED DNA-BINDING PROTEIN XP-E FACTOR SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NICHHD,DNA REPLICAT REPAIR & MUTAGENESIS SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 284 EP 284 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401014 ER PT J AU OKINAKA, RT PEREZ, A LAUBSCHER, K SENA, AP MACINNES, MA KRAEMER, KH AF OKINAKA, RT PEREZ, A LAUBSCHER, K SENA, AP MACINNES, MA KRAEMER, KH TI IDENTIFICATION OF MUTATIONS WITHIN THE ERCC-5 GENE IN A XERODERMA-PIGMENTOSUM GROUP-G PEDIGREE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 LOS ALAMOS NATL LAB,DIV LIFE SCI,LOS ALAMOS,NM 87545. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 285 EP 285 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401017 ER PT J AU UMAR, A BOYER, JC KUNKEL, TA AF UMAR, A BOYER, JC KUNKEL, TA TI A NOVEL DNA-REPAIR ACTIVITY CORRECTS UNPAIRED BASES IN MISMATCH REPAIR (+/-)-HUMAN CELL-FREE-EXTRACTS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 299 EP 299 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401073 ER PT J AU KOPSIDAS, G MACPHEE, DG AF KOPSIDAS, G MACPHEE, DG TI FRAMESHIFT MUTAGENESIS BY 9-AMINOACRIDINE - THE EFFECT OF MISMATCH REPAIR SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NATL INST CHILD HLTH DEV,BETHESDA,MD 20892. LA TROBE UNIV,DEPT MICROBIOL,BUNDOORA,VIC 3083,AUSTRALIA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 300 EP 300 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401078 ER PT J AU GORDENIN, D TRAN, H DEGTYAREVA, N KOLOTEVA, N RESNICK, M AF GORDENIN, D TRAN, H DEGTYAREVA, N KOLOTEVA, N RESNICK, M TI GENETIC-FACTORS AFFECTING REPLICATION SLIPPAGE BETWEEN DISTANT SHORT REPEATS IN YEAST SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 ST PETERSBURG STATE UNIV,ST PETERSBURG,RUSSIA. NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 307 EP 307 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401103 ER PT J AU MITAS, M DILL, J YU, AD KAMP, TJ CHOCK, JY HUANG, WJ AF MITAS, M DILL, J YU, AD KAMP, TJ CHOCK, JY HUANG, WJ TI A MAMMALIAN PROTEIN FORMS SALT-STABLE COMPLEXES WITH SINGLE-STRANDED-DNA FRAGMENTS THAT CONTAIN A MINIMUM OF 40 RESIDUES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 OKLAHOMA STATE UNIV,DEPT BIOCHEM & MOLEC BIOL,STILLWATER,OK 74078. JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV CARDIOL,BALTIMORE,MD 21205. NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 307 EP 307 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401104 ER PT J AU EVANS, MK CHIN, KV GOTTESMAN, MM BOHR, VA AF EVANS, MK CHIN, KV GOTTESMAN, MM BOHR, VA TI GENE-SPECIFIC DNA-REPAIR AND STEADY-STATE TRANSCRIPTION OF THE MDR1 GENE IN HUMAN TUMOR-CELL LINES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIA,BALTIMORE,MD 21224. UNIV MED & DENT NEW JERSEY,PISCATAWAY,NJ 08854. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 311 EP 311 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401120 ER PT J AU BOHR, VA APRHYS, C LEE, M JI, JP CULLINANE, C HJERTVIK, M MAZUR, S AF BOHR, VA APRHYS, C LEE, M JI, JP CULLINANE, C HJERTVIK, M MAZUR, S TI NUCLEOTIDE EXCISION-REPAIR PATHWAYS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIA,MOLEC GENET LAB,BALTIMORE,MD 21042. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 312 EP 312 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401123 ER PT J AU MAZUR, SJ HANKINSON, A BOHR, V AF MAZUR, SJ HANKINSON, A BOHR, V TI EFFECTS OF DOSE, ADDUCT DISTRIBUTION AND ADDUCT TYPE ON THE REPAIR OF 4-NITROQUINOLINE-1-OXIDE DAMAGE IN MAMMALIAN-CELLS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,GERONTOL RES CTR,MOLEC GENET LAB,BALTIMORE,MD 21220. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 315 EP 315 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401138 ER PT J AU BISWAS, I HSIEH, P AF BISWAS, I HSIEH, P TI PARAMETERS THAT INFLUENCE DNA BRANCH MIGRATION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 317 EP 317 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401143 ER PT J AU PERKINS, EL HASHEM, VI RESNICK, MA AF PERKINS, EL HASHEM, VI RESNICK, MA TI MANY HUMAN EXPRESSED GENES CAN BE CATEGORIZED ACCORDING TO PHENOTYPIC CONSEQUENCES IN THE YEAST RAD52 MUTANT, COMPROMISED FOR CHROMOSOME METABOLISM SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 322 EP 322 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401165 ER PT J AU BENNETT, CB WESTMORELAND, TJ SNIPE, JR RESNICK, M AF BENNETT, CB WESTMORELAND, TJ SNIPE, JR RESNICK, M TI LETHALITY, CHROMOSOME LOSS OR DELETION CAN RESULT FROM A SITE-SPECIFIC DOUBLE-STRAND BREAK WITHIN A DISPENSABLE HUMAN YAC IN YEAST SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. NR 1 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 331 EP 331 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401198 ER PT J AU KULAEVA, OI WOOTTON, JC LEVINE, AS WOODGATE, R AF KULAEVA, OI WOOTTON, JC LEVINE, AS WOODGATE, R TI CHARACTERIZATION OF THE UMU-COMPLEMENTING OPERON FROM R391 SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NICHHD,DNA REPLICAT REPAIR & MUTAGENESIS,BETHESDA,MD 20892. NIH,NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 332 EP 332 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401201 ER PT J AU KOCH, WH WOODGATE, R AF KOCH, WH WOODGATE, R TI IDENTIFICATION OF NEW UMUC HOMOLOGS BY DEGENERATE PRIMER PCR AMPLIFICATION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 US FDA,MOLEC BIOL BRANCH,WASHINGTON,DC 20204. NICHHD,DNA REPLICAT REPAIR & MUTAGENESIS SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 333 EP 333 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401205 ER PT J AU SEIDMAN, M LEVY, D AF SEIDMAN, M LEVY, D TI PROXIMAL AND DISTAL EFFECTS OF SEQUENCE CONTEXT ON ULTRAVIOLET MUTATIONAL HOTSPOTS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 OTSUKA PHARMACEUT CO LTD,ROCKVILLE,MD 20850. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 336 EP 336 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401218 ER PT J AU BEBENEK, K BEARD, WA DARDEN, TA WILSON, SH KUNKEL, TA AF BEBENEK, K BEARD, WA DARDEN, TA WILSON, SH KUNKEL, TA TI REDUCED FRAMESHIFT FIDELITY OF A REPLICATIVE POLYMERASE FROM HIV-1 CONTAINING MUTATIONS IN THE THUMB SUBDOMAIN SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. UNIV TEXAS,MED BRANCH,SEALY CTR MOLEC SCI,GALVESTON,TX 77555. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 337 EP 337 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401221 ER PT J AU KRAEMER, KH MORIWAKI, SI TARONE, RE TUCKER, MA GOLDSTEIN, AM AF KRAEMER, KH MORIWAKI, SI TARONE, RE TUCKER, MA GOLDSTEIN, AM TI ULTRAVIOLET HYPERMUTABILITY IN MELANOMA-PRONE FAMILIES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,MOLEC CARCINOGENESIS LAB,BALTIMORE,MD 21211. NCI,BIOSTAT BRANCH,BALTIMORE,MD 21211. NCI,GENET EPIDEMIOL BRANCH,BALTIMORE,MD 21211. RI Tucker, Margaret/B-4297-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 339 EP 339 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401229 ER PT J AU FORNACE, AJ ZHAN, QM CHEN, IT SMITH, ML AF FORNACE, AJ ZHAN, QM CHEN, IT SMITH, ML TI EVIDENCE FOR INVOLVEMENT OF THE P53 DNA-DAMAGE RESPONSE PATHWAY IN DNA-REPAIR SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,DCT,DTP,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 340 EP 340 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401234 ER PT J AU NAKAMURA, T PICHEL, JG WILLIAMSSIMONS, L WESTPHAL, H AF NAKAMURA, T PICHEL, JG WILLIAMSSIMONS, L WESTPHAL, H TI OVER-EXPRESSION OF WILD-TYPE AND A MUTANT HUMAN P53 IN THE LENS OF TRANSGENIC MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NICHHD,MAMMALIAN GENES & DEV LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 341 EP 341 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401238 ER PT J AU EPSTEIN, CJ HUANG, TT CARLSON, E CHAN, PH BRUNDIN, P NAKAO, N FRODL, E WIDNER, H CADET, JL AF EPSTEIN, CJ HUANG, TT CARLSON, E CHAN, PH BRUNDIN, P NAKAO, N FRODL, E WIDNER, H CADET, JL TI PROTECTIVE EFFECTS OF INCREASED EXPRESSION OF CUZN-SUPEROXIDE DISMUTASE IN THE CENTRAL-NERVOUS-SYSTEM OF TRANSGENIC MICE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDA,ADDICT RES CTR,BALTIMORE,MD 21224. UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. UNIV LUND HOSP,S-22185 LUND,SWEDEN. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 357 EP 357 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401286 ER PT J AU SNYDER, EY MACKLIS, ID WOLFE, JH GINNS, EI JENDOUBI, M SIDMAN, RL TESSLER, A PROIA, RL GOTTLIEB, DI FRIEDMANN, T YANDAVA, BD FLAX, JD AURORA, S YOON, CH MOZELL, RL PAN, ZH TAYLOR, RM MCKINNEY, C LACORAZZA, HD ROSARIO, CM KOSARAS, B KITCHENS, DL BAUM, L AF SNYDER, EY MACKLIS, ID WOLFE, JH GINNS, EI JENDOUBI, M SIDMAN, RL TESSLER, A PROIA, RL GOTTLIEB, DI FRIEDMANN, T YANDAVA, BD FLAX, JD AURORA, S YOON, CH MOZELL, RL PAN, ZH TAYLOR, RM MCKINNEY, C LACORAZZA, HD ROSARIO, CM KOSARAS, B KITCHENS, DL BAUM, L TI CNS PROGENITOR AND STEM-LIKE CELLS AS GENE DELIVERY VEHICLES AND MEDIATORS OF REPAIR SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD. HARVARD UNIV,SCH MED,DEPT MED GENET,BOSTON,MA 02115. UNIV PENN,SCH VET MED,PHILADELPHIA,PA 19104. NIMH,BETHESDA,MD 20892. NEI,BETHESDA,MD 20892. NEW ENGLAND REG PRIMATE RES CTR,SOUTHBOROUGH,MA 01772. MED COLL PENN,DEPT ANAT,PHILADELPHIA,PA 19129. WASHINGTON UNIV,DEPT ANAT & NEUROBIOL,ST LOUIS,MO 63130. UNIV CALIF SAN DIEGO,SCH MED,LA JOLLA,CA 92093. RI Proia, Richard/A-7908-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 357 EP 357 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401288 ER PT J AU FENIVES, ES BLAESE, RM TAICHMAN, LB AF FENIVES, ES BLAESE, RM TAICHMAN, LB TI CUTANEOUS GENE-THERAPY FOR ADA DEFICIENCY - A MODEL APPROACH FOR INHERITED METABOLIC DISORDERS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 SUNY STONY BROOK,DEPT ORAL BIOL & PATHOL,STONY BROOK,NY 11794. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 363 EP 363 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401307 ER PT J AU MARSH, JP LANZA, RP CHEN, L NELSON, DM MORGAN, RA CHICK, WL AF MARSH, JP LANZA, RP CHEN, L NELSON, DM MORGAN, RA CHICK, WL TI DELIVERY AND EXPRESSION OF HUMAN FACTOR-IX USING MICROREACTORS CONTAINING GENETICALLY-MODIFIED FIBROBLASTS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 BIOHYBRID TECHNOL INC,SHREWSBURY,MA 01545. NIH,NATL CTR HUMAN GENE RES,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 368 EP 368 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401325 ER PT J AU NELSON, DM METZGER, M DONAHUE, R MORGAN, RA AF NELSON, DM METZGER, M DONAHUE, R MORGAN, RA TI DIRECT IN-VIVO RETROVIRAL-MEDIATED GENE-TRANSFER INTO HEMATOPOIETIC PROGENITOR CELLS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,NATL CTR HUMAN GENOME RES,BETHESDA,MD 20892. NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 369 EP 369 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401330 ER PT J AU PUCK, J ISAKOV, J PEPPER, A ROSENBERG, F AF PUCK, J ISAKOV, J PEPPER, A ROSENBERG, F TI IL-2 RECEPTOR GAMMA-CHAIN MUTATIONS CAUSING HUMAN X-LINKED SCID ARE VARIABLE - SOME MAY BE DOMINANT NEGATIVES WHEN COEXPRESSED WITH WILD-TYPE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,NATL CTR HUMAN GENOME RES,GENE TRANSFER LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 370 EP 370 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401335 ER PT J AU FARINA, SF GIRARD, LJ VANIN, EF NIENHUIS, AW BODINE, DM AF FARINA, SF GIRARD, LJ VANIN, EF NIENHUIS, AW BODINE, DM TI DYSREGULATED EXPRESSION OF GATA-1 FOLLOWING RETROVIRAL TRANSFER INTO MURINE HEMATOPOIETIC STEM-CELLS INCREASES ERYTHROPOIESIS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH, NCHGR, HEMATOPOIESIS SECT, BETHESDA, MD 20892 USA. ST JUDE CHILDRENS RES HOSP, MEMPHIS, TN 38101 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 376 EP 376 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401357 ER PT J AU HENGGE, UR CHAN, EF FOSTER, RA WALKER, PS VOGEL, JC AF HENGGE, UR CHAN, EF FOSTER, RA WALKER, PS VOGEL, JC TI TRANSIENT GENE-EXPRESSION IN EPIDERMIS FOLLOWING INJECTION OF NAKED DNA SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,DERMATOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 377 EP 377 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401360 ER PT J AU OCONNELL, BC TENHAGEN, K TABAK, LA BAUM, BJ AF OCONNELL, BC TENHAGEN, K TABAK, LA BAUM, BJ TI ADENOVIRUS-MEDIATED DNA TRANSFER TO RAT SUBMANDIBULAR-GLAND IN-VIVO AND ANALYSIS OF GLUTAMINE-GLUTAMIC ACID-RICH PROTEIN-REGULATION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDR,CIPCB,BETHESDA,MD 20892. UNIV ROCHESTER,DEPT DENT RES,ROCHESTER,NY 14627. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 382 EP 382 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401379 ER PT J AU WALKER, PS HENGGE, UR VOGEL, JC AF WALKER, PS HENGGE, UR VOGEL, JC TI SIMULTANEOUS TRANSDUCTION OF KERATINOCYTES AND FIBROBLASTS WITH 2 RETROVIRAL VECTORS IN-VITRO SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,DERMATOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 386 EP 386 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401396 ER PT J AU LACORAZZA, HD FLAX, JD SNYDER, EY JENDOUBI, M AF LACORAZZA, HD FLAX, JD SNYDER, EY JENDOUBI, M TI IN-VIVO GENE-TRANSFER AND EXPRESSION OF HUMAN BETA-HEXOSAMINIDASE ALPHA-SUBUNIT INTO MOUSE-BRAIN SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NEI,IMMUNOL LAB,BETHESDA,MD 20892. HARVARD UNIV,SCH MED,DEPT NEUROL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 396 EP 396 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401434 ER PT J AU MORGAN, RA CHUAH, M VANDENDRIESSCHE, T BUNNELL, B BRESSLER, P WALKER, R LANE, C BLAESE, RM AF MORGAN, RA CHUAH, M VANDENDRIESSCHE, T BUNNELL, B BRESSLER, P WALKER, R LANE, C BLAESE, RM TI COMPARISON OF ANTI-HIV-1 RETROVIRAL VECTORS AND THEIR USE IN AN AIDS GENE-THERAPY TRIAL IN IDENTICAL-TWINS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NATL CTR HUMAN GENOME RES,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 3 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 397 EP 397 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401438 ER PT J AU NUSSBAUM, RL OLIVOS, I JANNE, P AF NUSSBAUM, RL OLIVOS, I JANNE, P TI THE OCULOCEREBRORENAL SYNDROME GENE ENCODES A PROTEIN THAT LOCALIZES TO THE GOLGI-COMPLEX SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIH,NATL CTR HUMAN GENOME RES,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 398 EP 398 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401443 ER PT J AU KIEHNTOPF, M BRACH, MA LICHT, T PETSCHAUER, S KARAWAJEW, L HERRMANN, F AF KIEHNTOPF, M BRACH, MA LICHT, T PETSCHAUER, S KARAWAJEW, L HERRMANN, F TI MDR-1 SPECIFIC RIBOZYMES - AN ANTI-GENE THERAPY APPROACH TO REVERSE THE DRUG-RESISTANT PHENOTYPE DURING ANTICANCER CHEMOTHERAPY SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,DEPT MED ONCOL & APPLE BIOL,W-1000 BERLIN 33,GERMANY. ROBERT ROSSLE KLIN,BERLIN,GERMANY. MAX DELBRUCK CTR MOLEC MED,BERLIN,GERMANY. NCI,DCBDC,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 423 EP 423 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401539 ER PT J AU FELZMANN, T RAMSEY, J BLAESE, RM AF FELZMANN, T RAMSEY, J BLAESE, RM TI DEVELOPMENT OF ANIMAL-MODELS FOR THE TREATMENT OF HUMAN PAPILLOMA-VIRUS INDUCED CARCINOMAS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCHGR,CLIN GENE THERAPY BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR 10 PY 1995 SU 21A BP 430 EP 430 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT864 UT WOS:A1995QT86401569 ER PT J AU WINKLER, JD HONG, BC BAHADOR, A KAZANIETZ, MG BLUMBERG, PM AF WINKLER, JD HONG, BC BAHADOR, A KAZANIETZ, MG BLUMBERG, PM TI METHODOLOGY FOR THE SYNTHESIS OF 3-OXYGENATED INGENANES - THE FIRST INGENOL ANALOGS WITH HIGH-AFFINITY FOR PROTEIN-KINASE-C SO JOURNAL OF ORGANIC CHEMISTRY LA English DT Article ID TUMOR PROMOTERS; UNSATURATED CENTERS; PHORBOL ESTERS; CYCLO-ADDITION; RING-SYSTEM; PROTOTYPE; RECEPTOR AB Previous work from our laboratories has demonstrated that the intramolecular dioxenone photocycloaddition reaction leads to a stereoselective synthesis of the carbocyclic ring system of the ingenane diterpenes with the ''inside-outside'' stereochemical relationship that is required for biological activity. Two different approaches for the synthesis of C-3 oxygenated analogs of ingenol and the preparation of the first ingenane analog with high affinity for protein kinase C are described. C1 NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,MOLEC MECHANISM TUMOR PROMOT SECT,BETHESDA,MD 20892. RP WINKLER, JD (reprint author), UNIV PENN,DEPT CHEM,PHILADELPHIA,PA 19104, USA. OI HONG, BOR-CHERNG/0000-0002-4623-3366 NR 25 TC 22 Z9 22 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA PO BOX 57136, WASHINGTON, DC 20037-0136 SN 0022-3263 J9 J ORG CHEM JI J. Org. Chem. PD MAR 10 PY 1995 VL 60 IS 5 BP 1381 EP 1390 DI 10.1021/jo00110a048 PG 10 WC Chemistry, Organic SC Chemistry GA QL605 UT WOS:A1995QL60500048 ER PT J AU PAUL, WE AF PAUL, WE TI AIDS RESEARCH POLICY SO SCIENCE LA English DT Letter RP PAUL, WE (reprint author), NIH,OFF AIDS RES,BLDG 10,BETHESDA,MD 20892, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC ADVAN SCIENCE PI WASHINGTON PA 1333 H ST NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 10 PY 1995 VL 267 IS 5203 BP 1405 EP 1406 DI 10.1126/science.7878451 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QL497 UT WOS:A1995QL49700002 PM 7878451 ER PT J AU BROWN, K GERSTBERGER, S CARLSON, L FRANZOSO, G SIEBENLIST, U AF BROWN, K GERSTBERGER, S CARLSON, L FRANZOSO, G SIEBENLIST, U TI CENTRAL OF I-KAPPA-B-ALPHA PROTEOLYSIS BY SITE-SPECIFIC, SIGNAL-INDUCED PHOSPHORYLATION SO SCIENCE LA English DT Article ID ONCOPROTEIN BCL-3; INHIBITION; PROTEINS; COMMON AB I kappa B-alpha inhibits transcription factor NF-kappa B by retaining it in the cytoplasm. Various stimuli, typically those associated with stress or pathogens, rapidly inactivate I kappa B-alpha. This liberates NF-kappa B to translocate to the nucleus and initiate transcription of genes important for the defense of the organism. Activation of NF-kappa B correlates with phosphorylation of I kappa B-alpha and requires the proteolysis of this inhibitor. When either serine-32 or serine-36 of I kappa B-alpha was mutated, the protein did not undergo signal-induced phosphorylation or degradation, and NF-kappa B could not be activated. These results suggest that phosphorylation at one or both of these residues is critical for activation of NF-kappa B. C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NR 19 TC 1247 Z9 1268 U1 1 U2 8 PU AMER ASSOC ADVAN SCIENCE PI WASHINGTON PA 1333 H ST NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 10 PY 1995 VL 267 IS 5203 BP 1485 EP 1488 DI 10.1126/science.7878466 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QL497 UT WOS:A1995QL49700034 PM 7878466 ER PT J AU CLORE, GM OMICHINSKI, JG SAKAGUCHI, K ZAMBRANO, N SAKAMOTO, H APPELLA, E GRONENBORN, AM AF CLORE, GM OMICHINSKI, JG SAKAGUCHI, K ZAMBRANO, N SAKAMOTO, H APPELLA, E GRONENBORN, AM TI INTERHELICAL ANGLES IN THE SOLUTION STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF P53 (VOL 265, PG 386, 1994) SO SCIENCE LA English DT Correction, Addition ID PROTEIN STRUCTURES; RESONANCE C1 NCI,CELL BIOL LAB,BETHESDA,MD 20892. RP CLORE, GM (reprint author), NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892, USA. RI Clore, G. Marius/A-3511-2008; Sakamoto, Hiroshi/A-3181-2011; Zambrano, Nicola/B-9352-2014 OI Clore, G. Marius/0000-0003-3809-1027; Zambrano, Nicola/0000-0001-9395-3481 NR 7 TC 66 Z9 75 U1 0 U2 5 PU AMER ASSOC ADVAN SCIENCE PI WASHINGTON PA 1333 H ST NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 10 PY 1995 VL 267 IS 5203 BP 1515 EP 1516 DI 10.1126/science.7878474 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QL497 UT WOS:A1995QL49700043 PM 7878474 ER PT J AU RUDENSEY, LM KIMATA, JT BENVENISTE, RE OVERBAUGH, J AF RUDENSEY, LM KIMATA, JT BENVENISTE, RE OVERBAUGH, J TI PROGRESSION TO AIDS IN MACAQUES IS ASSOCIATED WITH CHANGES IN THE REPLICATION, TROPISM, AND CYTOPATHIC PROPERTIES OF THE SIMIAN IMMUNODEFICIENCY VIRUS VARIANT POPULATION SO VIROLOGY LA English DT Article ID AMINO-ACID CHANGES; HUMAN CELL-LINES; MACROPHAGE TROPISM; ENVELOPE GENE; HOST-RANGE; NEUTRALIZATION EPITOPE; MONONUCLEAR PHAGOCYTES; SYNCYTIUM FORMATION; VIRAL DETERMINANTS; MOLECULAR CLONES AB Human immunodeficiency virus type 1 (HIV-1) typically evolves from a macrophage-tropic, noncytopathic virus al early asymptomatic stages of infection to a T-cell-tropic, cytopathic, and syncytia-inducing virus population as humans progress to AIDS. This suggests that changes in virus phenotype may influence disease. Because simian immunodeficiency virus (SIV) infection in macaques is a common model system for HIV-1 pathogenesis, we determined whether SIV infection in macaques that develop simian AIDS is associated with a similar shift in viral tropism, replication, and cytopathic properties. The virus that infected the monkeys (SIVMneCL8) and predominated at early times in infection is a macrophage-tropic virus that replicates with relatively low efficiency in human T cell lines. The variant populations that arise in macaques as they progress to AIDS are more infectious for human T cell lines, exhibiting enhanced replication in CEMX174 cells and an expanded host range that includes Molt-4 Clone 8 cells. Infections starting with equal doses of the viruses demonstrated that the late variants are cytopathic and syncytia-inducing compared to SIVMneCL8, but the variants replicate less efficiently in primary macaque macrophages. Vs sequences were generally conserved between the early and the late variants, suggesting that changes in SIVMne tropism, replication, and cytopathicity were apparently not due to alterations in V3. This study demonstrates important similarities in the phenotypic viral changes that accompany development of AIDS in SIV and HIV-1 infections and suggest that SIV may provide a model system for determining whether the rapidly replicating, T-cell-tropic cytopathic variants present late in infection and disease are indeed important in determining progression to AIDS. (C) 1995 Academic Press, Inc. C1 UNIV WASHINGTON,DEPT MICROBIOL,SEATTLE,WA 98195. NCI,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702. FU NCI NIH HHS [T32-CA09229]; NCRR NIH HHS [RR00166]; NIAID NIH HHS [R01 AI034251, R01 AI34251] NR 61 TC 57 Z9 58 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD MAR 10 PY 1995 VL 207 IS 2 BP 528 EP 542 DI 10.1006/viro.1995.1113 PG 15 WC Virology SC Virology GA QN321 UT WOS:A1995QN32100021 PM 7886956 ER PT J AU HONG, HL DEVEREUX, TR BOORMAN, GA SILLS, RC AF HONG, HL DEVEREUX, TR BOORMAN, GA SILLS, RC TI RAS ONCOGENE MUTATIONS IN HARDERIAN-GLAND NEOPLASMS OF B6C3F1 MICE EXPOSED TO ISOPRENE FOR 6 MONTHS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP 132 EP 132 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700796 ER PT J AU JONES, JE HANNIGAN, JL SHAW, GL LINNOILA, RI AF JONES, JE HANNIGAN, JL SHAW, GL LINNOILA, RI TI A NOVEL POLYMORPHISM OF THE HUMAN AROMATIC HYDROCARBON (AH) RECEPTOR - ASSOCIATION WITH INCREASED LUNG-CANCER RISK SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV S FLORIDA,H LEE MOFFITT CANC CTR,TAMPA,FL 33612. NCI,DCPC,BPRB,ROCKVILLE,MD 20850. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP 134 EP 134 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700806 ER PT J AU CLAUSEN, P DUNN, J BLAIR, D AF CLAUSEN, P DUNN, J BLAIR, D TI C-ETS-1 INDUCES DIFFERENTIATION OF ERYTHROLEUKEMIC CELL-LINES K562 AND HEL SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,LMO,FREDERICK,MD 21702. PRI DYNCORP,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP 138 EP 138 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700830 ER PT J AU RIVENSON, A RAO, CV STEELE, V KELLOFF, G REDDY, BS AF RIVENSON, A RAO, CV STEELE, V KELLOFF, G REDDY, BS TI INHIBITION OF 2-AMINO-1-METHYL-6-PHENYLIMIDAZO-[4,5-B]PYRIDINE (PHIP)-INDUCED LYMPHOMA BY OLTIPRAZ IN RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 AMER HLTH FDN,VALHALLA,NY 10595. NCI,BETHESDA,MD 20814. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP 139 EP 139 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700840 ER PT J AU HILL, EM BADER, T NETTESHEIM, P ELING, TE AF HILL, EM BADER, T NETTESHEIM, P ELING, TE TI SELECTIVE EXPRESSION OF PROSTAGLANDIN-H SYNTHASE (PGHS) ISOZYMES AND CYTOSOLIC PHOSPHOLIPASE A(2) (CPLA(2)) DURING DIFFERENTIATION OF RAT TRACHEAL EPITHELIAL (RTE) CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP 147 EP 147 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700882 ER PT J AU VIEIRA, NE OBRIEN, KO SPECKER, BL YERGEY, AL AF VIEIRA, NE OBRIEN, KO SPECKER, BL YERGEY, AL TI FRACTION OF DIET DERIVED URINARY CALCIUM UNCHANGED WITH LACTATION BUT INCREASED WITH HIGHER INTAKE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,BETHESDA,MD 20892. UNIV CINCINNATI,MED CTR,DEPT PEDIAT,CINCINNATI,OH 45229. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP 161 EP 161 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700963 ER PT J AU NEBELING, L FORMAN, M GRAUBARD, B AF NEBELING, L FORMAN, M GRAUBARD, B TI EFFECT OF HORMONAL USE AND LIFE-STYLE CHARACTERISTICS ON SPECIFIC AND TOTAL CAROTENOID INTAKE IN US WOMEN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,DIV CANC PREVENT & CONTROL,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP 171 EP 171 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701022 ER PT J AU ABAD, L RALL, L HARRIS, TB WILSON, PWF DINARELLO, CA ROUBENOFF, R AF ABAD, L RALL, L HARRIS, TB WILSON, PWF DINARELLO, CA ROUBENOFF, R TI INFLAMMATORY CYTOKINE PRODUCTION IN AGING - DIVERGENCE BETWEEN AGONIST AND ANTAGONIST RESPONSES SO FASEB JOURNAL LA English DT Meeting Abstract C1 TUFTS UNIV,HNRC,JMUSDA,BOSTON,MA 02111. TUFTS UNIV NEW ENGLAND MED CTR,BOSTON,MA 02111. FRAMINGHAM HEART DIS EPIDEMIOL STUDY,FRAMINGHAM,MA 01701. NIA,EDBP,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A182 EP A182 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701086 ER PT J AU BALLARDBARBASH, R GRAUBARD, B KREBSSMITH, S THOMPSON, F SCHATZKIN, A AF BALLARDBARBASH, R GRAUBARD, B KREBSSMITH, S THOMPSON, F SCHATZKIN, A TI CONTRIBUTION OF DIETING TO THE INVERSE ASSOCIATION BETWEEN ENERGY-INTAKE AND BODY-MASS INDEX SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A282 EP A282 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701659 ER PT J AU BOZZA, M KOLAKOWSKI, LF JENKINS, NA GILBERT, DJ COPELAND, NG DAVID, JR GERARD, C AF BOZZA, M KOLAKOWSKI, LF JENKINS, NA GILBERT, DJ COPELAND, NG DAVID, JR GERARD, C TI STRUCTURAL CHARACTERIZATION AND CHROMOSOMAL LOCATION OF THE MOUSE MACROPHAGE-MIGRATION INHIBITORY FACTOR (MIF) GENE AND 5 PSEUDOGENES SO FASEB JOURNAL LA English DT Meeting Abstract C1 CHILDRENS HOSP,BOSTON,MA 02115. HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. NCI,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A534 EP A534 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703113 ER PT J AU COLIGAN, JE BRANDO, C MARTINON, F SHEVACH, EM STURMHOFEL, K AF COLIGAN, JE BRANDO, C MARTINON, F SHEVACH, EM STURMHOFEL, K TI ANTIGEN-INDEPENDENT, INTEGRIN-MEDIATED T-CELL ACTIVATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LMS,BETHESDA,MD 20892. NIAID,LI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A233 EP A233 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701376 ER PT J AU CONNORS, M BOYLE, MJ FLANIGAN, ME GEIGER, SP FORD, H BASELER, M ADELSBERGER, J DAVEY, RT LANE, HC AF CONNORS, M BOYLE, MJ FLANIGAN, ME GEIGER, SP FORD, H BASELER, M ADELSBERGER, J DAVEY, RT LANE, HC TI THE HU-HIV/PBL-SCID MOUSE - A MODIFIED HU-PBL-SCID MODEL FOR THE STUDY OF HIV PATHOGENESIS AND THERAPY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LIR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A210 EP A210 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701249 ER PT J AU DAVENPECK, K CHREST, F BOCHNER, B AF DAVENPECK, K CHREST, F BOCHNER, B TI CARBOXYFLUORESCEIN DIACETATE (CFDA) LABELING DOES NOT AFFECT ADHESION MOLECULE (AM) EXPRESSION OR FUNCTION IN HUMAN EOSINOPHILS (EOS) OR NEUTROPHILS (PMN) SO FASEB JOURNAL LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,SCH MED,BALTIMORE,MD 21224. NIA,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A311 EP A311 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701828 ER PT J AU DISALVO, J KLEINMAN, HK NELSON, S AF DISALVO, J KLEINMAN, HK NELSON, S TI LAMININ, PLATELET-DERIVED GROWTH-FACTOR (PDGF) AND TYR-KINASE ACTIVITY PROMOTE NETWORKING OF VASCULAR SMOOTH-MUSCLE CELLS (VSMC) ON RECONSTITUTED BASEMENT-MEMBRANE SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MINNESOTA,DULUTH,MN 55812. NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A295 EP A295 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701731 ER PT J AU EBERLY, KW GHOSH, P YOUNG, H AF EBERLY, KW GHOSH, P YOUNG, H TI BIOCHEMICAL PATHWAYS OF FLAVONE-8-ACETIC ACID GENE INDUCTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,LEI,FREDERICK,MD 21701. ST MARYS COLL,NOTRE DAME,IN 46556. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A509 EP A509 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702973 ER PT J AU EBNET, K LUCE, GEG FARBER, JM SHAW, S AF EBNET, K LUCE, GEG FARBER, JM SHAW, S TI EXPRESSION OF THE MIG CXC CHEMOKINE GENE BY HUMAN ENDOTHELIAL-CELLS - SIMILARITIES AND DIFFERENCES TO OTHER CHEMOKINES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A199 EP A199 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701184 ER PT J AU ERSHOW, A AF ERSHOW, A TI DIETARY-EFFECTS ON LIPOPROTEINS AND THROMBOGENIC ACTIVITY - RATIONALE AND DESIGN OF THE DELTA STUDY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A289 EP A289 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701697 ER PT J AU FINOTTO, S OH, CK NAGATA, H METCALFE, DD AF FINOTTO, S OH, CK NAGATA, H METCALFE, DD TI EVIDENCE FOR TISSUE-SPECIFIC ALTERNATIVE SPLICING OF STEM-CELL FACTOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A488 EP A488 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702850 ER PT J AU FOGLER, WE MCCORMICK, KL VOLKER, K ORTALDO, JR WILTROUT, RH AF FOGLER, WE MCCORMICK, KL VOLKER, K ORTALDO, JR WILTROUT, RH TI NK CELL INFILTRATION INTO LUNG IS PRIMARILY REGULATED BY VCAM-1 INTERACTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 BRMP,LEI,EXPTL THERAPEUT SECT,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,BCDP,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A219 EP A219 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701300 ER PT J AU FREDERICK, DW HICKS, LH WRIGHT, JM AF FREDERICK, DW HICKS, LH WRIGHT, JM TI WHOLE-CELL PATCH-CLAMP STUDY OF ENZYME EFFECTS ON N-METHYL-D-ASPARTIC ACID (NMDA) RECEPTORS IN CULTURED MOUSE CORTICAL-NEURONS SO FASEB JOURNAL LA English DT Meeting Abstract C1 HOWARD UNIV,WASHINGTON,DC 20059. NIAAA,MOLEC & CELLULAR NEUROBIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A374 EP A374 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702189 ER PT J AU FREEMAN, S KING, JC VIEIRA, NE WOODHOUSE, LR YERGEY, AL AF FREEMAN, S KING, JC VIEIRA, NE WOODHOUSE, LR YERGEY, AL TI 41CA AS A TRACER FOR CALCIUM-METABOLISM IN HUMANS MEASURED BY ACCELERATOR MASS-SPECTROMETRY SO FASEB JOURNAL LA English DT Meeting Abstract C1 LAWRENCE LIVERMORE NATL LAB,LIVERMORE,CA 94551. UNIV CALIF BERKELEY,DEPT NUTR SCI,BERKELEY,CA 94720. NICHHD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A284 EP A284 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701668 ER PT J AU GLASGOW, WC EVERHART, AE AF GLASGOW, WC EVERHART, AE TI ROLE OF LINOLEIC AND ARACHIDONIC-ACID METABOLISM IN REGULATING MITOGENESIS IN ERBB-2 TRANSFORMED-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A113 EP A113 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700659 ER PT J AU GOLDING, B GOLDING, H INMAN, J SCOTT, DE AF GOLDING, B GOLDING, H INMAN, J SCOTT, DE TI HIV V3 PEPTIDE CONJUGATED TO BRUCELLA-ABORTUS IS IMMUNOGENIC IN CD4+ T-CELL DEFICIENT MICE SO FASEB JOURNAL LA English DT Meeting Abstract C1 US FDA,BETHESDA,MD 20892. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A207 EP A207 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701229 ER PT J AU GUEGUEN, M LONG, EO AF GUEGUEN, M LONG, EO TI THE PROCESSING PATHWAY FOR THE PRESENTATION OF CYTOSOLIC ANTIGEN BY MHC CLASS-II INVOLVES LONG-LIVED PROTEINS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOGENET LAB,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A530 EP A530 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703092 ER PT J AU HARRIS, HW FUKUYAMA, R AF HARRIS, HW FUKUYAMA, R TI NEURONAL DIFFERENTIATION OF PC12 CELLS IN THE ABSENCE OF EXTRACELLULAR-MATRIX ADHESION INDUCES APOPTOSIS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A581 EP A581 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703389 ER PT J AU HENKIN, RI MARTIN, BM DAL, WL AF HENKIN, RI MARTIN, BM DAL, WL TI A MAGNESIUM PROTEIN FOUND IN HUMAN PAROTID-SALIVA SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,CLIN NEUROSCI BRANCH,BETHESDA,MD 20205. TASTE & SMELL CLIN,WASHINGTON,DC 20016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A452 EP A452 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702646 ER PT J AU HONG, HL DEVEREUX, TR BOORMAN, GA SILLS, RC AF HONG, HL DEVEREUX, TR BOORMAN, GA SILLS, RC TI RAS ONCOGENE MUTATIONS IN HARDERIAN-GLAND NEOPLASMS OF B6C3F1 MICE EXPOSED TO ISOPRENE FOR 6 MONTHS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A132 EP A132 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700772 ER PT J AU HUI, R EVERHART, AL GLASGOW, WC AF HUI, R EVERHART, AL GLASGOW, WC TI EPIDERMAL GROWTH FACTOR-STIMULATED INCORPORATION OF 13(S)-HODE IS ASSOCIATED WITH TUMOR-SUPPRESSOR GENE PHENOTYPE IN SYRIAN-HAMSTER EMBRYO(SHE) FIBROBLASTS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A113 EP A113 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700662 ER PT J AU IRVINE, KR MCFARLAND, BJ ROSENBERG, SA RESTIFO, NP AF IRVINE, KR MCFARLAND, BJ ROSENBERG, SA RESTIFO, NP TI SYNTHETIC OLIGONUCLEOTIDE EXPRESSED BY RECOMBINANT VACCINIA VIRUS ELICITS THERAPEUTIC CYTOLYTIC T-LYMPHOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,SURG BRANCH,BETHESDA,MD 20892. RI Restifo, Nicholas/A-5713-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A494 EP A494 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702884 ER PT J AU ISSEKUTZ, TB TAUB, D SCHALL, TJ AF ISSEKUTZ, TB TAUB, D SCHALL, TJ TI EFFECT OF BETA-CHEMOKINES ON MONOCYTE AND LYMPHOCYTE RECRUITMENT IN-VIVO AND INHIBITION BY CD11/CD18 AND VLA-4 INTEGRIN BLOCKADE SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV TORONTO,TORONTO,ON,CANADA. NCI,FREDERICK,MD 21701. DNAX RES INST MOLEC & CELLULAR BIOL INC,PALO ALTO,CA 94304. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A270 EP A270 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701590 ER PT J AU JANKOVIC, D ASLUND, L OSWALD, LP CASPAR, P SHER, A JAMES, SL AF JANKOVIC, D ASLUND, L OSWALD, LP CASPAR, P SHER, A JAMES, SL TI CALPAIN IS THE TARGET ANTIGEN OF A TH1 CLONE WHICH TRANSFERS PROTECTIVE IMMUNITY AGAINST SCHISTOSOMA-MANSONI SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,PARASIT DIS LAB,IMMUNOBIOL SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A490 EP A490 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702862 ER PT J AU JUN, D PARK, HK LEE, YH KIM, Y NAGEL, J NORDIN, A AF JUN, D PARK, HK LEE, YH KIM, Y NAGEL, J NORDIN, A TI GENOMIC ORGANIZATION OF MURINE HOMOLOGS OF CDC2 AND CDK2 SO FASEB JOURNAL LA English DT Meeting Abstract C1 KYUNGPOOK NATL UNIV,DEPT MICROBIOL,TAEGU 702701,SOUTH KOREA. NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A233 EP A233 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701379 ER PT J AU KANT, AK BALLARDBARBASH, R SCHATZKIN, A AF KANT, AK BALLARDBARBASH, R SCHATZKIN, A TI EVENING EATING AND SUBSEQUENT WEIGHT CHANGE IN THE NHANES-I EPIDEMIOLOGIC FOLLOW-UP-STUDY (NHEFS) SO FASEB JOURNAL LA English DT Meeting Abstract C1 CUNY QUEENS COLL,FLUSHING,NY 11367. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A282 EP A282 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701661 ER PT J AU KAPLAN, D STEPHENS, R RASOULY, D MICHAUD, N PENG, X GREENE, L AF KAPLAN, D STEPHENS, R RASOULY, D MICHAUD, N PENG, X GREENE, L TI SIGNAL-TRANSDUCTION BY TRK RECEPTORS IN NEURONAL CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD. COLUMBIA UNIV,DEPT PATHOL,NEW YORK,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A267 EP A267 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701575 ER PT J AU KARI, F SLADE, R CRISSMAN, K LUSTER, M HATCH, G AF KARI, F SLADE, R CRISSMAN, K LUSTER, M HATCH, G TI DIETARY RESTRICTION MITIGATES OZONE-INDUCED LUNG TOXICITY IN RATS - ROLE OF ASCORBATE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27719. US EPA,RES TRIANGLE PK,NC 27719. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A413 EP A413 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702417 ER PT J AU KELLY, GD ORIJI, GK TATE, JE KEISER, HR AF KELLY, GD ORIJI, GK TATE, JE KEISER, HR TI ENDOTHELIN-INDUCED PROSTACYCLIN PRODUCTION IN RAT AORTIC RINGS IS MEDITATED BY PROTEIN-KINASE-C SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,HYPERTENS ENDOCRINE BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A110 EP A110 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700646 ER PT J AU KENNEY, MA MCCOY, H CHAPIN, RE KU, WW LIU, Y WILLIAMS, L AF KENNEY, MA MCCOY, H CHAPIN, RE KU, WW LIU, Y WILLIAMS, L TI MECHANICAL-PROPERTIES OF BONES OF RATS FED A COMPLETE DIET PLUS BORIC-ACID SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. UNIV ARKANSAS,FAYETTEVILLE,AR 72701. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A447 EP A447 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702611 ER PT J AU KIM, JH WESS, J SCHONEBERG, T JACOBSON, KA AF KIM, JH WESS, J SCHONEBERG, T JACOBSON, KA TI SITE-DIRECTED MUTAGENESIS IDENTIFIES RESIDUES INVOLVED IN LIGAND RECOGNITION IN THE HUMAN A(2A) ADENOSINE RECEPTOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,LBC,MOLEC RECOGNIT SECT,BETHESDA,MD 20892. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A122 EP A122 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700712 ER PT J AU KITZLER, JW REDDY, N ELING, TE AF KITZLER, JW REDDY, N ELING, TE TI CLONING SEQUENCING AND EXPRESSION OF A 5-LIPOXYGENASE ORTHOLOG FROM SYRIAN-HAMSTER EMBRYO FIBROBLASTS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,EICOSANOID BIOCHEM SECT,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A112 EP A112 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700657 ER PT J AU KOSHIBA, M APASOV, S CHEN, P SYERDLOV, V TURNER, J WEISSMAN, G SITKOVSKY, M AF KOSHIBA, M APASOV, S CHEN, P SYERDLOV, V TURNER, J WEISSMAN, G SITKOVSKY, M TI COMPARATIVE-STUDIES OF THE EXPRESSION OF THE P2U AND P2X PURINERGIC RECEPTORS IN DIFFERENT SUBPOPULATIONS OF T-CELLS AT DIFFERENT STAGES OF DIFFERENTIATION AND ACTIVATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. UNIV MISSOURI,COLUMBIA,MO 65212. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A116 EP A116 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98700681 ER PT J AU KWON, BS YOUN, BS KIM, SH BROXMEYER, HE JENKINS, N COPELAND, NG AF KWON, BS YOUN, BS KIM, SH BROXMEYER, HE JENKINS, N COPELAND, NG TI A NOVEL CHEMOKINE MRP-2 INHIBITS COLONY FORMATION OF BONE-MARROW MYELOID PROGENITORS SO FASEB JOURNAL LA English DT Meeting Abstract C1 INDIANA UNIV,SCH MED,INDIANAPOLIS,IN 46202. FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A246 EP A246 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701451 ER PT J AU LEDERER, JA LIOU, JS RICE, NR LICHTMAN, AH AF LEDERER, JA LIOU, JS RICE, NR LICHTMAN, AH TI DIFFERENTIAL NF-KAPPA-B REGULATION IN TH1 AND TH2 CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 BRIGHAM & WOMENS HOSP,BOSTON,MA 02115. NCI,FREDERICK CANC RES & DEV CTR,FREDERICK,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A536 EP A536 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703127 ER PT J AU LI, Z MIYASHITA, Y CHENG, L LAKATTA, E FROEHLICH, J AF LI, Z MIYASHITA, Y CHENG, L LAKATTA, E FROEHLICH, J TI REMODELING OF THE RAT AORTIC-WALL DURING AGING SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 4 Z9 4 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A606 EP A606 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703536 ER PT J AU LIAO, F RABIN, R YANNELLI, J KONIARIS, L VANGURI, P FARBER, J AF LIAO, F RABIN, R YANNELLI, J KONIARIS, L VANGURI, P FARBER, J TI HUMAN MIG IS A CXC CHEMOKINE THAT TARGETS ACTIVATED T-CELLS AND THAT IS PRODUCED AS A COLLECTION OF PROTEOLYTICALLY PROCESSED FORMS OF DIFFERING SPECIFIC ACTIVITIES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A199 EP A199 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701183 ER PT J AU LONGO, DL FUNAKOSHI, S ARMITAGE, RJ FANSLOW, WC MURPHY, WJ AF LONGO, DL FUNAKOSHI, S ARMITAGE, RJ FANSLOW, WC MURPHY, WJ TI INHIBITION OF HUMAN B-CELL LYMPHOMA GROWTH BY SOLUBLE RECOMBINANT HUMAN CD40 LIGAND SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,BRMP,LLB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,BCDP,FREDERICK,MD 21702. IMMUNEX RES & DEV CORP,SEATTLE,WA 98101. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A205 EP A205 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701218 ER PT J AU MANSOOR, A STEVENSON, MS WEISS, W AHMAN, M KHAN, A MUSHTAQ, S JAFFE, ES KINGMA, DW AF MANSOOR, A STEVENSON, MS WEISS, W AHMAN, M KHAN, A MUSHTAQ, S JAFFE, ES KINGMA, DW TI EPSTEIN-BARR-VIRUS (EBV) IS FREQUENTLY ASSOCIATED WITH SPORADIC AGGRESSIVE NON-HODGKINS (NHL) LYMPHOMA FROM PAKISTAN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. AFIP,RAWALPINDI,PAKISTAN. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A273 EP A273 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701605 ER PT J AU MASON, L ORTALDO, JR AF MASON, L ORTALDO, JR TI MAB 12A8 IDENTIFIES A UNIQUE SUBSET OF MURINE NK CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,BRMP,LEI,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A220 EP A220 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701301 ER PT J AU MCCARTNEYFRANCIS, N MIZEL, D REDMAN, R FRAZIERJESSEN, M PANEK, R KULKARNI, A WARD, J WAHL, S AF MCCARTNEYFRANCIS, N MIZEL, D REDMAN, R FRAZIERJESSEN, M PANEK, R KULKARNI, A WARD, J WAHL, S TI LOSS-OF-FUNCTION MUTATION OF THE TGF-BETA-1 GENE RESULTS SALIVARY-GLAND ABNORMALITIES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINCDS,NIDR,BETHESDA,MD 20878. VET ADM MED CTR,WASHINGTON,DC 20422. NCI,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A512 EP A512 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702986 ER PT J AU MCCOY, H KENNEY, MA CHAPIN, RE KU, WW LIU, Y WILLIAMS, L AF MCCOY, H KENNEY, MA CHAPIN, RE KU, WW LIU, Y WILLIAMS, L TI DOES GENDER AFFECT RESPONSE OF BONE TO SUPPLEMENTAL BORON SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV ARKANSAS,FAYETTEVILLE,AR 72701. NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A447 EP A447 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702614 ER PT J AU MONTUORI, N SIRI, J SIMMONS, T GILLET, C SENTERRE, G WRATHALL, L SOBEL, ME AF MONTUORI, N SIRI, J SIMMONS, T GILLET, C SENTERRE, G WRATHALL, L SOBEL, ME TI PRECURSOR-PRODUCT RELATIONSHIP BETWEEN A 37-KDA POLYPEPTIDE AND THE 67-KDA LAMININ RECEPTOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,PATHOL LAB,BETHESDA,MD 20892. RI Montuori, Nunzia/J-8542-2013 NR 0 TC 4 Z9 4 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A539 EP A539 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703144 ER PT J AU NEURATH, MF STUBER, ER MAX, EE STROBER, W AF NEURATH, MF STUBER, ER MAX, EE STROBER, W TI THE TRANSCRIPTION FACTOR BSAP REPRESSES THE IMMUNOGLOBULIN HEAVY-CHAIN 3'ALPHA ENHANCER IN-VIVO BY INHIBITION OF BINDING OF NF-ALPHA-P, AN ETS-LIKE PROTEIN THAT CONTROLS IG GENE-TRANSCRIPTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LCI,MIS,BETHESDA,MD 20892. US FDA,CYBER,CELL & VIRAL REGULAT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A196 EP A196 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701169 ER PT J AU NOVOTNY, JA DUEKER, SR ZECH, LA CLIFFORD, AJ AF NOVOTNY, JA DUEKER, SR ZECH, LA CLIFFORD, AJ TI THE KINETICS OF BETA-CAROTENE METABOLISM IN HUMANS SO FASEB JOURNAL LA English DT Meeting Abstract C1 USDA,BELTSVILLE HUMAN NUTR RES CTR,RES LAB,BELTSVILLE,MD 20705. NCI,BETHESDA,MD 20892. UNIV CALIF DAVIS,DAVIS,CA 95616. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A443 EP A443 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702590 ER PT J AU OH, CK METCALFE, DD AF OH, CK METCALFE, DD TI IDENTIFICATION AND CHARACTERIZATION OF NEGATIVE REGULATORY ELEMENTS OF T-CELL ACTIVATION GENE-3 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A199 EP A199 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701182 ER PT J AU ORIJI, GK KELLY, GD PIERUZZI, FU KEISER, HR AF ORIJI, GK KELLY, GD PIERUZZI, FU KEISER, HR TI EFFECT OF PROTEIN-KINASE-C ON CONTRACTIONS EVOKED BY ENDOTHELIN IN RAT AORTIC RINGS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,HYPERTENS ENDOCRINE BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A615 EP A615 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703590 ER EF