FN Thomson Reuters Web of Science™ VR 1.0 PT J AU SCHWARZ, U MILES, FA AF SCHWARZ, U MILES, FA TI OCULAR RESPONSES TO TRANSLATION AND THEIR DEPENDENCE ON VIEWING DISTANCE .1. MOTION OF THE OBSERVER SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID EYE-MOVEMENT RESPONSES; LINEAR ACCELERATION; OTOLITH ORGANS; HEAD; REFLEX; DISPLACEMENT; STIMULATION; VERGENCE; SYSTEM AB 1. The horizontal eye movements induced by acceleration along the interaural axis were recorded from five monkeys (Macaca mulatta) by the use of the electromagnetic search-coil technique. Animals sat on a sled that was moved briefly in darkness along a linear track (bell-shaped acceleration profile: peak, 400 mm/s2; duration, 200 ms). Immediately before acceleration, animals fixated one of five target lights located at distances ranging from 16 to 150 cm. During fixation, the horizontal positions of both eyes were used to check vergence, while accommodation was monitored with an infrared optometer. 2. Sled motion induced eye movements that were generally smooth and compensatory, e.g., rightward sled motion elicited leftward eye movement. We attribute these responses to a translational vestibuloocular reflex (TVOR) that senses the sled motion through the otolith organs. However, in three animals, these responses were preceded by weak anticompensatory movements (duration, < 40 ms; amplitude, < 10% of the maximum compensatory response). 3. Geometry indicates that, during brief sled motion, the eye movements required to keep gaze aligned on a particular location ("full" compensation) are inversely proportional to the viewing distance. Response measures based on the computed eye velocity profiles, such as the velocity achieved at specific times ("time slices") or the peak values of the estimated covariance functions, all indicated that compensatory responses were a linear function of the inverse of the prior viewing distance. Cross-correlation analyses indicated that the effect of the prior viewing distance was to scale responses, although detailed spectral analyses revealed that high-frequency components (> 10 Hz) tended to scale less vigorously than lower ones. 4. The adequacy of the compensatory eye movements was assessed by calculating the gain (response recorded/response required for full compensation). Regardless of the response measure used, gains varied considerably from one animal to another and, in some particular animals, from one direction to another but showed a general tendency to increase with viewing distance. For example, on the basis of the peak eye velocity achieved within 250 ms of the onset of sled motion, mean gain at 16 cm was 0.74 (range, 0.48-1.01), whereas at 150 cm it was 1.25 (range, 0.67-1.73). 5. Using wedge prisms to dissociate vergence and accommodation indicated that ocular responses to sled motion were sensitive to selective increases in either vergence (base-out prism with the most distant target) or accommodation (base-in prism with the nearest target). However, the magnitude of the effects showed considerable variability from one animal to another and, in some particular animals, from one direction to another. Further, neither vergence nor accommodation alone nor a linear combination of the two cues could account for all of the data. 6. We conclude that 1) translational accelerations can elicit robust compensatory eye movements that show the required linear dependence on proximity, although with considerable intersubject variability in the impoverished conditions used here (darkness); 2) vergence and accommodation cues are used to modulate compensatory eye movements in accordance with viewing distance, but they are often not the only cues used. C1 NEI,SENSORIMOTOR RES LAB,BETHESDA,MD 20892. NR 53 TC 161 Z9 162 U1 0 U2 4 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD SEP PY 1991 VL 66 IS 3 BP 851 EP 864 PG 14 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA GE056 UT WOS:A1991GE05600016 PM 1753290 ER PT J AU BUSETTINI, C MILES, FA SCHWARZ, U AF BUSETTINI, C MILES, FA SCHWARZ, U TI OCULAR RESPONSES TO TRANSLATION AND THEIR DEPENDENCE ON VIEWING DISTANCE .2. MOTION OF THE SCENE SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID TERM ADAPTIVE-CHANGES; PRIMATE VESTIBULOOCULAR REFLEX; OPTOKINETIC NYSTAGMUS; MONKEY AB 1. The ocular following responses induced by brief (100-ms) movements of the visual scene were examined for their dependence on viewing distance in 5 monkeys (Macaca mulatta). The horizontal positions of both eyes and the vertical position of one eye were recorded using the electromagnetic search-coil technique. Accommodation was monitored in selected experiments by use of an infrared optometer. Test patterns (random dots) were back-projected onto a translucent tangent screen facing the animal. Six viewing distances were used (range, 20-150 cm), the size and speed of the image on the screen being adjusted for each so as to preserve a constant retinal image. 2. Response measures based on the amplitude of the first peak in the eye acceleration profile or the eye velocity achieved at specific times all indicated that ocular following responses were inversely related to viewing distance, the relationship being linear for the earliest measures. On average, the sensitivity to viewing distance was comparable with that reported for the translational vestibuloocular reflex (TVOR) in the preceding paper: as viewing distance increased from 20 cm, ocular following decremented at a mean rate (+/- SD) of 17 +/- 3% per m-1, while the TVOR decremented at a mean rate (+/- SD) of 18 +/- 1% per m-1. 3. Ocular following responses showed the postsaccadic enhancement described by Kawano and Miles regardless of viewing distance. To a first approximation, the effects of postsaccadic enhancement and viewing distance summed linearly. 4. The dependence of ocular following on speed showed the progressive saturation previously described by Miles et al. at all viewing distances, the peak eye velocity achieved being inversely related to the viewing distance, indicating that the saturation must originate upstream of the dependence on viewing distance. Under normal viewing conditions, this speed saturation will tend to offset the dependence on viewing distance because the retinal slip speeds experienced by the moving observer will tend to vary inversely with viewing distance, resulting in greater saturation with nearer viewing. 5. Wedge prisms were used to dissociate vergence and accommodation and indicated that ocular following responses were sensitive to selective increases in either vergence (base-out prism with the screen at 100 cm) or accommodation (base-in prism with the screen at 20 cm). However, as with the TVOR, the magnitude of the effects showed considerable variability from one animal to another and, in some particular animals, from one direction to another. Further, neither vergence nor accommodation alone nor a linear combination of the two cues could account for all of the data. 6. Our data are consistent with the idea that the early component of ocular following and the TVOR share some common structure (a central pathway with efficacy modulated by various cues to viewing distance) and function (compensation for translational disturbances of the observer). C1 UNIV TRIESTE,DIPARTIMENTO ELETTROTECN ELETTRON & INFORMAT,I-34100 TRIESTE,ITALY. RP BUSETTINI, C (reprint author), NEI,SENSORIMOTOR RES LAB,BETHESDA,MD 20892, USA. NR 26 TC 56 Z9 56 U1 1 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD SEP PY 1991 VL 66 IS 3 BP 865 EP 878 PG 14 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA GE056 UT WOS:A1991GE05600017 PM 1753291 ER PT J AU ELMALLAKH, RS KIRCH, DG SHELTON, R FAN, KJ PEZESHKPOUR, G KANHOUWA, S WYATT, RJ KLEINMAN, JE AF ELMALLAKH, RS KIRCH, DG SHELTON, R FAN, KJ PEZESHKPOUR, G KANHOUWA, S WYATT, RJ KLEINMAN, JE TI THE NUCLEUS BASALIS OF MEYNERT, SENILE PLAQUES, AND INTELLECTUAL IMPAIRMENT IN SCHIZOPHRENIA SO JOURNAL OF NEUROPSYCHIATRY AND CLINICAL NEUROSCIENCES LA English DT Article ID ALZHEIMERS-DISEASE; CHOLINERGIC INNERVATION; DEMENTIA; CORTEX AB The large, hyperchromic, cholinergic neurons of the nucleus basalis of Meynert (nbM) and the presence of senile plaques were quantified in postmortem brain tissue from 10 intellectually impaired schizophrenic patients, seven intellectually intact schizophrenic patients, seven control subjects, and three patients with Alzheimer's disease. The two groups of schizophrenic patients did not show any significant differences when compared with the control group in nbM cell density or in plaque frequency. The Alzheimer's disease patients showed the expected decrease in nbM neuronal density and increase in plaques compared with the controls. The data suggest that compromised cognitive function in schizophrenia is not associated with diffuse neuropathology of the basal forebrain cholinergic system. C1 NIMH,NEUROPSYCHIAT BRANCH,ROCKVILLE,MD 20857. NIMH,CLIN BRAIN DISORDERS BRANCH,ROCKVILLE,MD 20857. ST ELIZABETH HOSP,CTR NEUROSCI,WASHINGTON,DC 20032. ST ELIZABETH HOSP,BLACKBURN LAB,WASHINGTON,DC 20032. NR 19 TC 42 Z9 42 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0895-0172 J9 J NEUROPSYCH CLIN N JI J. Neuropsychiatr. Clin. Neurosci. PD FAL PY 1991 VL 3 IS 4 BP 383 EP 386 PG 4 WC Clinical Neurology; Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA GM866 UT WOS:A1991GM86600005 PM 1821257 ER PT J AU FREED, WJ AF FREED, WJ TI BRAIN-TISSUE GRAFTING AND HUMAN APPLICATIONS SO JOURNAL OF NEUROSURGICAL ANESTHESIOLOGY LA English DT Editorial Material ID PARKINSONS-DISEASE; CONTROLLED RELEASE; ADRENAL-MEDULLA; DOPAMINE; STRIATUM; TRANSPLANTATION; NEURONS; SYSTEM; MOTOR; CELLS RP FREED, WJ (reprint author), NIMH,NEUROSCI CTR ST ELIZABETHS,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 27 TC 2 Z9 2 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0898-4921 J9 J NEUROSURG ANESTH JI J. Neurosurg. Anesthesiol. PD SEP PY 1991 VL 3 IS 3 BP 167 EP 169 DI 10.1097/00008506-199109000-00001 PG 3 WC Anesthesiology; Clinical Neurology; Surgery SC Anesthesiology; Neurosciences & Neurology; Surgery GA GD661 UT WOS:A1991GD66100001 PM 10147763 ER PT J AU LARSON, SM CARRASQUILLO, JA COLCHER, DC YOKOYAMA, K REYNOLDS, JC BACHARACH, SA RAUBITCHEK, A PACE, L FINN, RD ROTMAN, M STABIN, M NEUMANN, RD SUGARBAKER, P SCHLOM, J AF LARSON, SM CARRASQUILLO, JA COLCHER, DC YOKOYAMA, K REYNOLDS, JC BACHARACH, SA RAUBITCHEK, A PACE, L FINN, RD ROTMAN, M STABIN, M NEUMANN, RD SUGARBAKER, P SCHLOM, J TI ESTIMATES OF RADIATION ABSORBED DOSE FOR INTRAPERITONEALLY ADMINISTERED I-131 RADIOLABELED B72.3 MONOCLONAL-ANTIBODY IN PATIENTS WITH PERITONEAL CARCINOMATOSES SO JOURNAL OF NUCLEAR MEDICINE LA English DT Article ID F(AB')2 FRAGMENTS; COLON CANCER; TUMOR; RADIOIMMUNOTHERAPY; CELLS; MICE AB Using a newly available model for determining estimates of radiation absorbed dose of radioisotopes administered intraperitoneally, we have calculated absorbed dose to tumor and normal tissues based on a surgically controlled study of radiolabeled antibody distribution. Ten patients with peritoneal carcinomatosis received intraperitoneal injections of the murine monoclonal antibody B72.3 radiolabeled with I-131. Biodistribution studies were performed using nuclear medicine methods until laparotomy at 4-14 days after injection. Surgical biopsies of normal tissues and tumor were obtained. The marrow was predicted to be the critical organ, with maximum tolerated dose [200 rad (2 Gy) to marrow] expected at about 200 mCi (7.4 GBq). In patients with large intraperitoneal tumor deposits, the tumor itself is an important source tissue for radiation exposure to normal tissues. Local "hot-spots" for tumor-absorbed dose were observed, with maximum tumor-absorbed dose calculated at 11000 rad (11 Gy) per 100 mCi (3.7 GBq) administered intraperitoneal; however, tumor rad dose varied considerably. This may pose serious problems for curative therapy, especially in patients with large tumor burdens. C1 NIH,CTR RADIOL,DEPT NUCL MED,BETHESDA,MD 20892. NIH,SURG ONCOL BRANCH,DIV CANC BIOL,TUMOR IMMUNOL & BIOL BRANCH,BETHESDA,MD 20892. NIH,DIV CANC TREATMENT,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. OAK RIDGE ASSOCIATED UNIV,CTR RADIOPHARMACEUT INTERNAL DOSE INFORMAT,OAK RIDGE,TN 37830. RP LARSON, SM (reprint author), MEM SLOAN KETTERING CANC CTR,NUCL MED SERV,1275 YORK AVE,NEW YORK,NY 10021, USA. RI Carrasquillo, Jorge/E-7120-2010; OI Pace, Leonardo/0000-0002-5741-543X; Carrasquillo, Jorge/0000-0002-8513-5734 NR 24 TC 31 Z9 32 U1 0 U2 2 PU SOC NUCLEAR MEDICINE INC PI RESTON PA 1850 SAMUEL MORSE DR, RESTON, VA 22090-5316 SN 0161-5505 J9 J NUCL MED JI J. Nucl. Med. PD SEP PY 1991 VL 32 IS 9 BP 1661 EP 1667 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA GD043 UT WOS:A1991GD04300005 PM 1880565 ER PT J AU GREEN, MV BACHARACH, SL BORER, JS BONOW, RO AF GREEN, MV BACHARACH, SL BORER, JS BONOW, RO TI A THEORETICAL COMPARISON OF 1ST-PASS AND GATED EQUILIBRIUM METHODS IN THE MEASUREMENT OF SYSTOLIC LEFT-VENTRICULAR FUNCTION SO JOURNAL OF NUCLEAR MEDICINE LA English DT Article ID CORONARY-ARTERY DISEASE; EJECTION FRACTION; RADIONUCLIDE ANGIOCARDIOGRAPHY; HEART-DISEASE; WALL MOTION; EXERCISE; ANGIOGRAPHY; REST; CINEANGIOGRAPHY; NITROGLYCERIN AB First-pass and gated equilibrium radionuclide studies of left ventricular function have proven extremely useful in the detection and management of patients with heart disease. Despite this practical experience, however, comparison of these methods generally has been confined to procedural differences that do not reflect the intrinsic properties of the methods. Here, we describe the results of a simple theoretical calculation from first principles that compares the methods based on their relative statistical precision. This analysis assumes that each procedure is carried out with the same tracer dose in the same hypothetical patient under identical conditions and with the same ideal imaging equipment. Results obtained with this model suggest that the imaging time required for a gated equilibrium study to achieve the same statistical precision as a first-pass study is typically less than 2 min in resting subjects and less than 1 min during stress. The analysis also indicates that gated equilibrium studies will tend to possess the greater statistical precision when cardiac output is elevated, such as when the heart is imaged during exercise. On the other hand, this analysis indicates that the first-pass method will tend to possess the greater precision when cardiac output is low and when imaging time is highly constrained. C1 CORNELL UNIV,MED CTR,NEW YORK HOSP,NEW YORK,NY 10021. RP GREEN, MV (reprint author), NIH,ROOM 1C401,BLDG 10,BETHESDA,MD 20892, USA. NR 18 TC 6 Z9 6 U1 0 U2 0 PU SOC NUCLEAR MEDICINE INC PI RESTON PA 1850 SAMUEL MORSE DR, RESTON, VA 22090-5316 SN 0161-5505 J9 J NUCL MED JI J. Nucl. Med. PD SEP PY 1991 VL 32 IS 9 BP 1801 EP 1807 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA GD043 UT WOS:A1991GD04300032 PM 1880584 ER PT J AU WONG, FCL BOJA, JW WONG, DF WILSON, AA CARROLL, FI KUHAR, MJ WAGNER, HN AF WONG, FCL BOJA, JW WONG, DF WILSON, AA CARROLL, FI KUHAR, MJ WAGNER, HN TI X-RAY AFFINITY LABELING OF DOPAMINE TRANSPORTER BY PHOTOAFFINITY LIGANDS SO JOURNAL OF NUCLEAR MEDICINE LA English DT Meeting Abstract C1 NIDA,ARC,JOHNS HOPKINS MED INST,BALTIMORE,MD. RES TRIANGLE INST,RES TRIANGLE PK,NC 27709. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SOC NUCLEAR MEDICINE INC PI RESTON PA 1850 SAMUEL MORSE DR, RESTON, VA 22090-5316 SN 0161-5505 J9 J NUCL MED JI J. Nucl. Med. PD SEP PY 1991 VL 32 IS 9 BP 1839 EP 1839 PG 1 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA GD043 UT WOS:A1991GD04300066 ER PT J AU LEE, KS SASSAMAN, MB DECOSTA, BR RADESCA, L BRAUN, A BOWEN, WD RICE, KC WEINBERGER, D AF LEE, KS SASSAMAN, MB DECOSTA, BR RADESCA, L BRAUN, A BOWEN, WD RICE, KC WEINBERGER, D TI RADIOIODINATION OF A HIGHLY SPECIFIC SIGMA RECEPTOR LIGAND AS A POTENTIAL SPECT IMAGING AGENT SO JOURNAL OF NUCLEAR MEDICINE LA English DT Meeting Abstract C1 NIMH,CBDB,WASHINGTON,DC 20032. NIDDKD,MED CHEM LAB,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NUCLEAR MEDICINE INC PI RESTON PA 1850 SAMUEL MORSE DR, RESTON, VA 22090-5316 SN 0161-5505 J9 J NUCL MED JI J. Nucl. Med. PD SEP PY 1991 VL 32 IS 9 BP 1841 EP 1841 PG 1 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA GD043 UT WOS:A1991GD04300074 ER PT J AU REZNIK, VM ADAMSON, M ADELMAN, RD MURPHY, JL GAHL, WA CLARK, KF SCHNEIDER, JA AF REZNIK, VM ADAMSON, M ADELMAN, RD MURPHY, JL GAHL, WA CLARK, KF SCHNEIDER, JA TI TREATMENT OF CYSTINOSIS WITH CYSTEAMINE FROM EARLY INFANCY SO JOURNAL OF PEDIATRICS LA English DT Article ID NEPHROPATHIC CYSTINOSIS; URINE SAMPLES; THERAPY; PHOSPHOCYSTEAMINE; PROTEINURIA; DEPLETION; PLASMA C1 UNIV CALIF SAN DIEGO, DEPT PEDIAT 0609F, 9500 GILMAN DR, LA JOLLA, CA 92093 USA. NICHHD, HUMAN GENET BRANCH, BETHESDA, MD 20892 USA. UNIV CALIF DAVIS, SACRAMENTO MED CTR, DEPT PEDIAT, DIV PEDIAT NEPHROL, SACRAMENTO, CA 95817 USA. FU NCRR NIH HHS [RR 00827]; NICHD NIH HHS [HD-6-2927]; NIDDK NIH HHS [DK 18434] NR 13 TC 16 Z9 16 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-3476 EI 1097-6833 J9 J PEDIATR-US JI J. Pediatr. PD SEP PY 1991 VL 119 IS 3 BP 491 EP 493 DI 10.1016/S0022-3476(05)82072-4 PG 3 WC Pediatrics SC Pediatrics GA GE057 UT WOS:A1991GE05700029 PM 1880670 ER PT J AU SMITH, RN ANDERSEN, RN KOLENBRANDER, PE AF SMITH, RN ANDERSEN, RN KOLENBRANDER, PE TI INHIBITION OF INTERGENERIC COAGGREGATION AMONG ORAL BACTERIA BY CETYLPYRIDINIUM CHLORIDE, CHLORHEXIDINE DIGLUCONATE AND OCTENIDINE DIHYDROCHLORIDE SO JOURNAL OF PERIODONTAL RESEARCH LA English DT Article DE CETYLPYRIDINIUM CHLORIDE; CHLORHEXIDINE DIGLUCONATE; OCTENIDINE DIHYDROCHLORIDE; COAGGREGATION; SUBGINGIVAL BACTERIA ID HUMAN EXPERIMENTAL GINGIVITIS; HUMAN DENTAL PLAQUE; ACTINOMYCES-NAESLUNDII; SURFACE RECOGNITION; STANNOUS FLUORIDE; CELL-INTERACTIONS; SP-NOV; COLONIZATION; BACTEROIDES; STREPTOCOCCI AB The potential inhibitory effect of chlorhexidine digluconate on the intergeneric coaggregation of 11 pairs of Gram-positive organisms was compared to its ability to inhibit coaggregations of 14 pairs comprised of both a Gram-positive and a Gram-negative cell type. Dramatic differences in the inhibitory effectiveness of the antimicrobial compound on the two kinds of coaggregating pairs were found. Gram-positive pairs were not inhibited at a concentration of 0.25%, whereas the coaggregations involving a Gram-negative partner were usually completely blocked at concentrations as low as 0.01%. Similar effects to chlorhexidine digluconate were found with octenidine dihydrochloride and cetylpyridinium chloride, while sodium dodecylsulfate was inhibitory only at 10- to 50-fold higher concentrations. These results suggest that chlorhexidine digluconate, octenidine dihydrochloride, and cetylpyridinium chloride may be effective inhibitors of later microbial colonizers of dental plaque but may not disturb a normal healthy indigenous flora. C1 NIDR,MICROBIAL ECOL LAB,BLDG 30,ROOM 310,BETHESDA,MD 20892. NR 41 TC 18 Z9 18 U1 0 U2 4 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0022-3484 J9 J PERIODONTAL RES JI J. Periodont. Res. PD SEP PY 1991 VL 26 IS 5 BP 422 EP 428 DI 10.1111/j.1600-0765.1991.tb01732.x PG 7 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA GD478 UT WOS:A1991GD47800007 PM 1832455 ER PT J AU STONE, AA KESSLER, RC HAYTHORNTHWAITE, JA AF STONE, AA KESSLER, RC HAYTHORNTHWAITE, JA TI MEASURING DAILY EVENTS AND EXPERIENCES - DECISIONS FOR THE RESEARCHER SO JOURNAL OF PERSONALITY LA English DT Article ID SITUATIONS; RESPONSES; BIAS C1 UNIV MICHIGAN,INST SOCIAL RES,ANN ARBOR,MI 48106. NIA,BALTIMORE,MD 21224. RP STONE, AA (reprint author), SUNY STONY BROOK,DEPT PSYCHIAT,STONY BROOK,NY 11794, USA. FU NIMH NIH HHS [K01-MH00507, R01-MH39234, M01-MH42714] NR 39 TC 138 Z9 139 U1 10 U2 18 PU DUKE UNIV PRESS PI DURHAM PA BOX 90660, DURHAM, NC 27708-0660 SN 0022-3506 J9 J PERS JI J. Pers. PD SEP PY 1991 VL 59 IS 3 BP 575 EP 607 DI 10.1111/j.1467-6494.1991.tb00260.x PG 33 WC Psychology, Social SC Psychology GA GF739 UT WOS:A1991GF73900010 PM 1960643 ER PT J AU OLEARY, KM COWDRY, RW GARDNER, DL LEIBENLUFT, E LUCAS, PB DEJONGMEYER, R AF OLEARY, KM COWDRY, RW GARDNER, DL LEIBENLUFT, E LUCAS, PB DEJONGMEYER, R TI DYSFUNCTIONAL ATTITUDES IN BORDERLINE PERSONALITY-DISORDER SO JOURNAL OF PERSONALITY DISORDERS LA English DT Article ID PSYCHIATRIC-INPATIENTS; COGNITIVE ASSESSMENT; DEPRESSED-PATIENTS; TREATMENT RESPONSE; VALIDITY; PATTERNS; THERAPY; STYLE C1 GEORGETOWN UNIV HOSP,DEPT PSYCHIAT,WASHINGTON,DC 20007. RP OLEARY, KM (reprint author), NIMH,CTR NEUROSCI,ROOM 562,WASHINGTON,DC 20032, USA. NR 38 TC 16 Z9 17 U1 1 U2 1 PU GUILFORD PUBLICATIONS INC PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0885-579X J9 J PERS DISORD JI J. Pers. Disord. PD FAL PY 1991 VL 5 IS 3 BP 233 EP 242 PG 10 WC Psychiatry SC Psychiatry GA GK755 UT WOS:A1991GK75500003 ER PT J AU YEH, SY AF YEH, SY TI METABOLIC PROFILE OF TRIPELENNAMINE IN HUMANS SO JOURNAL OF PHARMACEUTICAL SCIENCES LA English DT Article ID RAT; PENTAZOCINE; PYRILAMINE AB Volunteers were injected im with 100 mg of tripelennamine (pyribenzamine) . CHl dissolved in saline. Timed urine was collected. Tripelennamine and its metabolites were identified by GC/MS. Amounts of free tripelennamine excreted in the 0-2-, 2-4-, 4-8-, 8-12-, and 12-24-h urine samples were found to be 0.30, 0.56, 0.17, 0.21, and 0.0%, respectively, of the administered dose. In the same time periods, total tripelennamine (free plus conjugated) amounts were found to be 0.92, 1.20, 0.96, 1.30, and 1.31%, respectively, and total amounts 2-[alpha-hydroxybenzyl(2-dimethylaminoethyl)amino]pyridine(alpha-hydroxytripelennamine) plus an unidentified metabolite were found to be 0.16, 3.35, 3.06, 7.46, and 8.85% of the dose, respectively. RP YEH, SY (reprint author), NIDA,ADDICT RES CTR,POB 5180,BALTIMORE,MD 21224, USA. NR 14 TC 3 Z9 3 U1 0 U2 0 PU AMER PHARMACEUTICAL ASSN PI WASHINGTON PA 2215 CONSTITUTION AVE NW, WASHINGTON, DC 20037 SN 0022-3549 J9 J PHARM SCI JI J. Pharm. Sci. PD SEP PY 1991 VL 80 IS 9 BP 815 EP 819 DI 10.1002/jps.2600800902 PG 5 WC Chemistry, Medicinal; Chemistry, Multidisciplinary; Pharmacology & Pharmacy SC Pharmacology & Pharmacy; Chemistry GA GE769 UT WOS:A1991GE76900001 PM 1800700 ER PT J AU CONTRERAS, PC JACOBSON, AE RICE, KC FARAH, JM AF CONTRERAS, PC JACOBSON, AE RICE, KC FARAH, JM TI PHENCYCLIDINE INCREASED RELEASE OF BETA-ENDORPHIN FROM ANTERIOR LOBE OF THE PITUITARY SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID CENTRAL NERVOUS-SYSTEM; STEREOTYPED BEHAVIOR; LUTEINIZING-HORMONE; LOCOMOTOR-ACTIVITY; RAT PROLACTIN; RECEPTOR; PCP; SIGMA; CORTICOSTERONE; BINDING AB Phencyclidine (PCP) has been found to affect neuroendocrine function by altering the release of the anterior pituitary hormones, adrenocorticotrophin, luteinizing hormone and prolactin. The purpose of this study was to examine the effect of PCP on release of the two pituitary hormones also derived from the adrenocorticotropin precursor, namely, alpha-melanocyte-stimulating hormone and beta-endorphin (beta-E), synthesized in the neurointermediate and anterior lobes of the pituitary. At behaviorally active doses, PCP administered i.c.v. increased plasma levels of immunoreactive beta-E(i-beta-E) without affecting the concentration of immunoreactive alpha-melanocyte-stimulating hormone, suggesting that PCP increased the release of beta-E from only the anterior lobe of the pituitary. Dexamethasone pretreatment blocked the PCP-induced increase in i-beta-E which indicated further the anterior lobe effects of PCP. MK-801 {(+)-5-methyl-10,11-dihydro-5H-dibenzo [a,d]cyclohepten-5,10-imine maleate}, a selective PCP ligand, at behaviorally active doses also increased the plasma concentration of i-beta-E. The dose-response curves for induction of behavior was very different from that for increasing the concentration of i-beta-E in plasma. The increase in release of i-beta-E was stereoselective as (+)-(1-(1-phenylcyclohexyl)-3 methylpiperidine but not (-)-(1-(1-phenylcyclohexyl)-3 methylpiperidine increased release of i-beta-E. The increase in plasma levels of beta-E was not due to an interaction with opioid receptors because naloxone did not block PCP-induced release of beta-E. In vitro, PCP also significantly increased release of i-beta-E from anterior lobe of the pituitary. These results indicate that PCP increased the release of i-beta-E exclusively from the anterior lobe corticotropes in vivo through an interaction, in part, with PCP receptors. C1 CENT NERVOUS SYST DIS RES,SEARLE RES & DEV,ST LOUIS,MO. NIDDKD,MED CHEM LAB,DRUG DESIGN & SYNTH SECT,BETHESDA,MD. RP CONTRERAS, PC (reprint author), CENT NERVOUS SYST DIS RES,SEARLE RES & DEV,4901 SEARLE PKWY K-2,SKOKIE,IL 60077, USA. NR 36 TC 3 Z9 3 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD SEP PY 1991 VL 258 IS 3 BP 837 EP 841 PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GF688 UT WOS:A1991GF68800014 PM 1653842 ER PT J AU ALI, H MULLER, CE DALY, JW BEAVEN, MA AF ALI, H MULLER, CE DALY, JW BEAVEN, MA TI METHYLXANTHINES BLOCK ANTIGEN-INDUCED RESPONSES IN RBL-2H3 CELLS INDEPENDENTLY OF ADENOSINE RECEPTORS OR CYCLIC-AMP - EVIDENCE FOR INHIBITION OF ANTIGEN-BINDING TO IGE SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID BASOPHILIC LEUKEMIA-CELLS; MAST-CELLS; HISTAMINE-RELEASE; MEDIATOR RELEASE; IMMUNOGLOBULIN-E; PHOSPHOLIPASE-C; CHOLERA-TOXIN; INOSITOL 1,4,5-TRISPHOSPHATE; PHOSPHOINOSITIDE BREAKDOWN; SYNERGISTIC SIGNALS AB Activation of a novel adenosine receptor in a rat tumor mast cell line (RBL-2H3 cells) elicits a transient generation of inositol 1,4,5-trisphosphate and an equally transient increase in the level of free cytosol Ca++: Such responses promote little exocytosis, but markedly enhance the secretory response to antigen. A variety of xanthine adenosine receptor antagonists did not suppress the responses to the adenosine analog 5-N-ethylcarboxamidoadenosine. However, 3-isobutyl-1-methylxanthine (IBMX) and certain related xanthines inhibited antigen (dinitrophenylated bovine serum albumin, DNP-BSA)-induced generation of inositol phosphates, the increase in level of free cytosolic Ca++ and exocytosis in RBL-2H3 cells that were primed with a monoclonal DNP-specific immunoglobulin E (from hybridoma H1 DNP-epsilon-26.82). The same compounds inhibited the binding of antigen to cell attached DNP-specific IgE in a highly selective manner. Incorporation of an aromatic or cycloalkyl group in the 8-position of IBMX or theophylline, for example, resulted in compounds that were more potent inhibitors than the parent compounds. Conversely, substituents in the 7- or 9-position of IBMX resulted in inactive compounds. 1,3-Diethylxanthine and 1,3-dipropylxanthine had no activity, suggesting that substituents as large as ethyl or propyl are not tolerated at the 1-position. Inhibition by IBMX was not observed when cells were activated by nonimmunological stimulants or when cells were primed with certain other monoclonal preparations of DNP-specific IgE and stimulated by DNP-BSA. Thus, the adenosine receptors in RBL-2H3 cells are distinguishable from A1 and A2 receptors by their resistance to xanthines, but some of the xanthines can interfere with antigen-induced responses. C1 NIDDKD, BIOORGAN CHEM LAB, BETHESDA, MD USA. RP NHLBI, CHEM PHARMACOL LAB, BETHESDA, MD 20892 USA. RI Muller, Christa/C-7748-2014 OI Muller, Christa/0000-0002-0013-6624 NR 49 TC 19 Z9 19 U1 0 U2 0 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 0022-3565 EI 1521-0103 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD SEP PY 1991 VL 258 IS 3 BP 954 EP 962 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GF688 UT WOS:A1991GF68800031 PM 1716313 ER PT J AU MCMILLAN, DE HARDWICK, WC DECOSTA, BR RICE, KC AF MCMILLAN, DE HARDWICK, WC DECOSTA, BR RICE, KC TI EFFECTS OF DRUGS THAT BIND TO PCP AND SIGMA RECEPTORS ON PUNISHED RESPONDING SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID DISCRIMINATIVE STIMULUS PROPERTIES; PHENCYCLIDINE ANALOGS; PIGEONS; BEHAVIOR; RAT; KETAMINE AB Several arylcyclohexylamines and opioid benzomorphans that bind to phencyclidine (PCP) receptors were studied for their effects on punished and unpunished responding maintained under fixed-interval schedules of food presentation. All of these drugs increased both punished and unpunished responding, although higher doses decreased responding. The order of potency for increasing punished responding was MK-801 [(+)-5-methyl-10,11-dihydro-5H-dibenzo(a,d)-cyclohepten-5,10-imine] > {1-[1-(2-thienyl)cyclohexyl]piperidine} = PCP > (+)-N-allylnormetazocine = (-)-N-allynormetazocine. There was a high correlation (0.95) between the relative potency of these drugs in increasing punished responding and their relative affinity for PCP receptors. Because some of these drugs also bind to sigma receptors, drugs with a high affinity for sigma receptors, such as haloperidol, BD 737 {1S,2R-(-)-cis-N-[2-(3,4-dichlorophenyl)ethyl]-N-methyl-2-(1-pyrrolidinyl) cyclohexylamine} and (+)-3-(3-hydroxyphenyl)-N-(1-propyl)piperidine, were also studied for their effects on punished and unpunished responding. These drugs produced only rate-decreasing effects. The correlation between the relative potency of drugs in increasing punished responding and their relative affinity for sigma receptors was low (-0.19). These data suggest that the PCP receptor is involved in some drug-induced increases in punished responding. C1 NIDDKD,MED CHEM LAB,BETHESDA,MD. RP MCMILLAN, DE (reprint author), UNIV ARKANSAS MED SCI HOSP,COLL MED,DEPT PHARMACOL & TOXICOL,4301 W MARKHAM ST,LITTLE ROCK,AR 72205, USA. FU NIDA NIH HHS [R01-DA02251-14] NR 25 TC 17 Z9 17 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD SEP PY 1991 VL 258 IS 3 BP 1015 EP 1018 PG 4 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GF688 UT WOS:A1991GF68800039 PM 1653832 ER PT J AU BRENNER, B YU, LC AF BRENNER, B YU, LC TI CHARACTERIZATION OF RADIAL FORCE AND RADIAL STIFFNESS IN CA2+-ACTIVATED SKINNED FIBERS OF THE RABBIT PSOAS MUSCLE SO JOURNAL OF PHYSIOLOGY-LONDON LA English DT Article ID CROSS-BRIDGE KINETICS; X-RAY-DIFFRACTION; RELAXED MUSCLE; RIGOR MUSCLE; FIBERS; CROSSBRIDGES; CONTRACTION; LATTICE; ANALOGS AB 1. When chemically skinned muscle fibres are activated by Ca2+ at an ionic strength of 170 mM, the spacing between the filaments has been shown to decrease with increasing force, suggesting that the cross-bridges can generate force not only in the axial but also in the radial direction. In the present study, radial force and radial stiffness of activated single skinned rabbit psoas fibres were studied by X-ray diffraction. The responses of the lattice spacing to changes in osmotic pressure by application of dextran T500, which is equivalent to force applied in the radial direction, was examined. The radial force generated by the attached cross-bridges was calculated, with the approximation that a negligible fraction of cross-bridges was attached in the relaxed muscle at the same ionic strength of 170 mM. 2. The active radial force was found to be a slightly non-linear function of lattice spacing, reaching zero at 34 nm. The radial force was compressive at lattice spacing greater than 34 nm and expansive at less than 34 nm. 3. The active axial force, on the other hand, was found to be much less affected by the application of dextran T500. Active axial force increased by 4% to a plateau at 4% dextran T500 and then decreased by 10% at 8% dextran T500. 4. While not under osmotic pressure, the radial force of the activated fibre was determined to be 400 pN (single thick filament)-1. This is of the same order of magnitude as the axial force. The radial stiffness was also comparable to the axial stiffness at 7 pN (thick filament)-1 (0.1 nm)-1. 5. The radial elasticity of the fully activated fibre differs significantly from that of the fiber in rigor. The radial stiffness exhibited by fibres in rigor was approximately five times higher, at 30 pN (thick filament)-1 (0.1 nm)-1 and the point where the radial force reached zero was 38 nm. 6. In the activated state, the point at which radial force reaches zero is independent of the level of Ca2+ activation, i.e. independent of the number of cross-bridges attached to actin in the force-generating state. We suggest that the zero-force point is equivalent to the equilibrium point of a spring and is an intrinsic property of the radial elasticity of the cross-bridge. 7. It is concluded that activated and rigor cross-bridges exhibit a spring-like property in the radial direction. Furthermore, the equilibrium point of the radial elasticity appears to depend on the physiological state of the cross-bridges. The significance of the large magnitude of radial elasticity and of the different equilibrium points is discussed. C1 NIH,BETHESDA,MD 20892. UNIV TUBINGEN,W-7400 TUBINGEN 1,GERMANY. NR 28 TC 49 Z9 49 U1 1 U2 1 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 0022-3751 J9 J PHYSIOL-LONDON JI J. Physiol.-London PD SEP PY 1991 VL 441 BP 703 EP 718 PG 16 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA GE461 UT WOS:A1991GE46100040 PM 1816390 ER PT J AU LEWIS, EN KALASINSKY, VF LEVIN, IW AF LEWIS, EN KALASINSKY, VF LEVIN, IW TI NEAR-INFRARED FIBEROPTIC SAMPLE CELL - APPLICATIONS TO FOURIER-TRANSFORM RAMAN AND NEAR-INFRARED ABSORPTION AND REFLECTANCE SPECTROSCOPIES SO JOURNAL OF RAMAN SPECTROSCOPY LA English DT Article ID SPECTROMETER AB A fiber-optic sample cell designed for use in the near-infrared region is described. For Fourier transform (FT) Raman spectroscopy, Nd:YAG laser radiation is transmitted to the sample through one fiber bundle, while a second fiber bundle collects and transfers the scattered light to the source compartment of an infrared interferometer equipped with a CaF2 beam splitter and an InGaAs detector. Because the two fiber bundles are mounted in the aluminium body of the cell, virtually no optical alignment is required. In addition to its utility in FT-Raman spectroscopy, the cell may be used for obtaining near-infrared absorption spectra of liquids and reflectance spectra of solids without changing its configuration. Examples of the many uses of the cell are provided, and modifications which would potentially improve its efficiency and expand its capabilities are discussed. C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. NR 25 TC 4 Z9 4 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0377-0486 J9 J RAMAN SPECTROSC JI J. Raman Spectrosc. PD SEP PY 1991 VL 22 IS 9 BP 509 EP 512 DI 10.1002/jrs.1250220907 PG 4 WC Spectroscopy SC Spectroscopy GA GG032 UT WOS:A1991GG03200006 ER PT J AU SCHIEWE, MC FITZ, TA BROWN, JL STUART, LD WILDT, DE AF SCHIEWE, MC FITZ, TA BROWN, JL STUART, LD WILDT, DE TI RELATIONSHIP OF ESTRUS SYNCHRONIZATION METHOD, CIRCULATING HORMONES, LUTEINIZING-HORMONE AND PROSTAGLANDIN-F2-ALPHA RECEPTORS AND LUTEAL PROGESTERONE CONCENTRATION TO PREMATURE LUTEAL REGRESSION IN SUPEROVULATED SHEEP SO JOURNAL OF REPRODUCTION AND FERTILITY LA English DT Article DE SHEEP; SUPEROVULATION; PREMATURE LUTEAL REGRESSION; PROSTAGLANDIN F2-ALPHA; RECEPTOR ID OVINE CORPUS-LUTEUM; ESTROUS-CYCLE; CORPORA-LUTEA; CELL-TYPES; MORPHOMETRIC ANALYSIS; SUPER-OVULATION; EARLY-PREGNANCY; LIFE-SPAN; EWES; GOATS AB Ewes were treated with exogenous follicle-stimulating hormone (FSH) and oestrus was synchronized using either a dual prostaglandin F-2-alpha (PGF-2-alpha) injection regimen or pessaries impregnated with medroxy progesterone acetate (MAP). Natural cycling ewes served as controls. After oestrus or AI (Day 0), corpora lutea (CL) were enucleated surgically from the left and right ovaries on Days 3 and 6, respectively. The incidence of premature luteolysis was related (P < 0.05) to PGF-2-alpha treatment and occurred in 7 of 8 ewes compared with 0 of 4 controls and 1 of 8 MAP-exposed females. Sheep with regressing CL had lower circulating and intraluteal progesterone concentrations and fewer total and small dissociated luteal cells on Day 3 than gonadotrophin-treated counterparts with normal CL. Progesterone concentration in the serum and luteal tissue was higher (P < 0.05) in gonadotrophin-treated ewes with normal CL than in the controls; but luteinizing hormone (LH) receptors/cell were not different on Days 3 and 6. There were no apparent differences in the temporal patterns of circulating oestradiol-17-beta, FSH and LH. High progesterone in gonadotrophin-treated ewes with normal CL coincided with an increase in total luteal mass and numbers of cells, which were primarily reflected in more small luteal cells than in control ewes. Gonadotrophin-treated ewes with regressing CL on Day 3 tended (P < 0.10) to have fewer small luteal cells and fewer (P < 0.05) low-affinity PGF-2-alpha binding sites than sheep with normal CL. By Day 6, luteal integrity and cell viability was absent in ewes with prematurely regressed CL. These data demonstrate that (i) the incidence of premature luteal regression is highly correlated with the use of PGF-2-alpha; (ii) this abnormal luteal tissue is functionally competent for 2-3 days after ovulation, but deteriorates rapidly thereafter and (iii) luteal-dysfunctioning ewes experience a reduction in numbers of small luteal cells without a significant change in luteal mass by Day 3 and, overall, have fewer low-affinity PGF-2-alpha binding sites. C1 NIH,NATL CTR RES RESOURCES,VET RESOURCES PROGRAM,BETHESDA,MD 20892. UNIFORMED SERV UNIV HLTH SCI,BETHESDA,MD 20814. RP SCHIEWE, MC (reprint author), SMITHSONIAN INST,NATL ZOOL PK,WASHINGTON,DC 20008, USA. NR 40 TC 23 Z9 25 U1 1 U2 2 PU J REPROD FERTIL INC PI CAMBRIDGE PA 22 NEWMARKET RD, CAMBRIDGE, ENGLAND CB5 8DT SN 0022-4251 J9 J REPROD FERTIL JI J. Reprod. Fertil. PD SEP PY 1991 VL 93 IS 1 BP 19 EP 30 PG 12 WC Reproductive Biology SC Reproductive Biology GA GG006 UT WOS:A1991GG00600005 PM 1920289 ER PT J AU ALARCON, GS WILLIAMS, GV SINGER, JZ STEEN, VD CLEGG, DO PAULUS, HE BILLINGSLEY, LM LUGGEN, ME POLISSON, RP WILLKENS, RF YARBORO, C MA, KN EGGER, MJ WILLIAMS, HJ WARD, JR AF ALARCON, GS WILLIAMS, GV SINGER, JZ STEEN, VD CLEGG, DO PAULUS, HE BILLINGSLEY, LM LUGGEN, ME POLISSON, RP WILLKENS, RF YARBORO, C MA, KN EGGER, MJ WILLIAMS, HJ WARD, JR TI EARLY UNDIFFERENTIATED CONNECTIVE-TISSUE DISEASE .1. EARLY CLINICAL MANIFESTATION IN A LARGE COHORT OF PATIENTS WITH UNDIFFERENTIATED CONNECTIVE-TISSUE DISEASES COMPARED WITH COHORTS OF WELL ESTABLISHED CONNECTIVE-TISSUE DISEASE SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE CONNECTIVE TISSUE DISEASES; MIXED; UNDIFFERENTIATED; COHORT ID SYSTEMIC LUPUS-ERYTHEMATOSUS; RHEUMATOID-ARTHRITIS; ANTIPHOSPHOLIPID SYNDROME; POLYMYOSITIS; EPIDEMIOLOGY; SCLEROSIS; SCLERODERMA; INVOLVEMENT; ANTIBODIES; FEATURES AB We identified a cohort of 410 patients with connective tissue disorders (CTD) of less-than-or-equal-to 1 year duration among the participating clinics of the Cooperative Systematic Studies of the Rheumatic Diseases Program. Fifty-seven had rheumatoid arthritis (RA), 57 systemic lupus erythematosus, 37 poly/dermatomyositis, 46 scleroderma, and 213 early undifferentiated CTD, including patients with Raynaud's phenomenon, unexplained polyarthritis or at least 3 CTD manifestations such as rashes, myalgias, etc. Baseline clinical data are now being reported. The followup of these patients may prove to be valuable in understanding these diseases. To our knowledge no similar cohort of patients is available for further investigation. C1 UNIV UTAH,CTR COORDINATING,COOPERAT SYST STUDIES RHEUMAT DIS PROGRAM,SALT LAKE CITY,UT 84112. SUNY HLTH SCI CTR,BROOKLYN,NY. UNIV PITTSBURGH,PITTSBURGH,PA 15260. UNIV CALIF LOS ANGELES,LOS ANGELES,CA 90024. JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. UNIV CINCINNATI,CINCINNATI,OH 45221. DUKE UNIV,DURHAM,NC 27706. UNIV WASHINGTON,SEATTLE,WA 98195. NIH,BETHESDA,MD 20892. RP ALARCON, GS (reprint author), UNIV ALABAMA,615 MEB,UAB STN,BIRMINGHAM,AL 35294, USA. FU NIADDK NIH HHS [1-AM6-2228] NR 32 TC 69 Z9 71 U1 0 U2 2 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD SEP PY 1991 VL 18 IS 9 BP 1332 EP 1339 PG 8 WC Rheumatology SC Rheumatology GA GK150 UT WOS:A1991GK15000012 PM 1757934 ER PT J AU CLEGG, DO WILLIAMS, HJ SINGER, JZ STEEN, VD SCHLEGEL, S ZIMINSKI, C ALARCON, GS LUGGEN, ME POLISSON, RP WILLKENS, RF YARBORO, C MCDUFFIE, FC WARD, JR AF CLEGG, DO WILLIAMS, HJ SINGER, JZ STEEN, VD SCHLEGEL, S ZIMINSKI, C ALARCON, GS LUGGEN, ME POLISSON, RP WILLKENS, RF YARBORO, C MCDUFFIE, FC WARD, JR TI EARLY UNDIFFERENTIATED CONNECTIVE-TISSUE DISEASE .2. THE FREQUENCY OF CIRCULATING ANTINUCLEAR ANTIBODIES IN PATIENTS WITH EARLY RHEUMATIC DISEASES SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE EARLY CONNECTIVE TISSUE DISEASE; ANTINUCLEAR ANTIBODIES; RHEUMATOID ARTHRITIS; SYSTEMIC LUPUS ERYTHEMATOSUS; SCLERODERMA; POLYMYOSITIS ID SYSTEMIC LUPUS-ERYTHEMATOSUS; DERMATOMYOSITIS; POLYMYOSITIS; SCLEROSIS AB The presence of antinuclear antibodies (ANA) in the serum is a common finding in various connective tissue disorders, but usefulness of these antibodies in making diagnoses or prognoses is not known. We report the results of a panel of ANA determinations including ANA, anti-dsDNA, Sm, RNP, SSA, SSB, Jo-1, Scl-70 and PM-1 in 410 patients in a 5-year descriptive study of 410 patients with rheumatic disease symptoms of less than one year's duration. While some patients met diagnostic criteria for a specific rheumatologic diagnosis, others were classified as undifferentiated connective tissue disease (UCTD) and were subclassified by a constellation of symptoms. Our results show that ANA is sensitive in systemic lupus erythematosus (SLE) and progressive systemic sclerosis even in early disease but is not specific. Other "specific" autoantibodies were seen most frequently in SLE but were relatively insensitive and were seen in low frequency in UCTD. ANA have limited diagnostic value in patients with early disease. The prognostic value of these tests will be assessed as the prospective study of these cohorts progresses. C1 UNIV UTAH,CTR CORDINATING,COOPERAT SYST STUDIES RHEUMAT DIS PROGRAM,SALT LAKE CITY,UT 84132. UNIV ALABAMA,BIRMINGHAM,AL 35294. SUNY HLTH SCI CTR,BROOKLYN,NY. UNIV PITTSBURGH,PITTSBURGH,PA 15260. UNIV CALIF LOS ANGELES,LOS ANGELES,CA 90024. JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. UNIV CINCINNATI,CINCINNATI,OH 45221. DUKE UNIV,DURHAM,NC 27706. UNIV WASHINGTON,SEATTLE,WA 98195. NIH,BETHESDA,MD 20892. CTR DIS CONTROL,ARTHRIT FDN,CONTROL ANA REFERENCE LAB,ATLANTA,GA 30333. FU NIADDK NIH HHS [1-AM6-2228] NR 25 TC 58 Z9 58 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD SEP PY 1991 VL 18 IS 9 BP 1340 EP 1343 PG 4 WC Rheumatology SC Rheumatology GA GK150 UT WOS:A1991GK15000013 PM 1757935 ER PT J AU SILVERMAN, ED LAXER, RM NELSON, DL RUBIN, LA AF SILVERMAN, ED LAXER, RM NELSON, DL RUBIN, LA TI SOLUBLE INTERLEUKIN-2 RECEPTOR IN JUVENILE RHEUMATOID-ARTHRITIS SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE DISEASE ACTIVITY; JRA; SOLUBLE IL-2 RECEPTOR; T-CELL ACTIVATION ID DISEASE-ACTIVITY; ANTI-TAC; T-CELLS; ACTIVATION; INVITRO; PROTEIN; INVIVO; IL-2 AB Juvenile rheumatoid arthritis (JRA) is a chronic, relapsing, inflammatory childhood disease characterized by arthritis and systemic inflammation. At present there is no rapid, efficient laboratory method of assessing disease activity and degree of immune activation. We measured serum soluble interleukin 2 receptor (sIL-2R) levels in 85 samples from 72 patients (22 samples from patients with systemic JRA, 34 from polyarticular patients, 29 from pauciarticular patients, of which 10 were HLA-B27 positive). The mean sIL-2R level from patients was 1565 U/ml, which is significantly elevated compared to control values of 594 U/ml (p less-than-or-equal-to 0.005). The highest levels were seen in patients with systemic JRA (mean value 2121 U/ml) while the lowest values were seen in HLA-B27 positive (+) patients (mean value 899 U/ml). Patients with clinically active disease had significantly elevated levels (mean value 1745 U/ml) compared to patients with inactive disease (mean value 846 U/ml, p less-than-or-equal-to 0.01). Highest levels were seen in patients with active systemic JRA (mean value 2419 U/ml) while patients with pauciarticular JRA and B27+ JRA had the lowest sIL-2R levels (1167 and 1045 U/ml, respectively). sIL-2R levels were elevated in all subgroups of clinically active patients compared to controls (p less-than-or-equal-to 0.0005). Three of the 4 patients with serial sIL-2R measurements showed falling values during the period of clinical remission. Using regression analysis and likelihood ratio tests, we found a significant correlation between sIL-2R levels and both disease activity and joint count (p less-than-or-equal-to 0.005). This correlation was significant even after accounting for the contribution of other laboratory measures of disease activity, including hemoglobin and albumin levels. We conclude that serum sIL-2R levels are an accurate and rapid measure of disease activity in patients with JRA and likely reflect both the systemic and local joint inflammation of this disease. C1 HOSP SICK CHILDREN,DIV RHEUMATOL,TORONTO M5G 1X8,ONTARIO,CANADA. SUNNYBROOK MED CTR,DIV RHEUMATOL,TORONTO,ONTARIO,CANADA. UNIV TORONTO,DEPT PEDIAT MED & IMMUNOL,TORONTO M5S 1A1,ONTARIO,CANADA. NCI,METAB BRANCH,BETHESDA,MD 20892. NR 23 TC 20 Z9 20 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD SEP PY 1991 VL 18 IS 9 BP 1398 EP 1402 PG 5 WC Rheumatology SC Rheumatology GA GK150 UT WOS:A1991GK15000024 PM 1757944 ER PT J AU NORRIS, FH KANIASTY, K AF NORRIS, FH KANIASTY, K TI THE PSYCHOLOGICAL EXPERIENCE OF CRIME - A TEST OF THE MEDIATING ROLE OF BELIEFS IN EXPLAINING THE DISTRESS OF VICTIMS SO JOURNAL OF SOCIAL AND CLINICAL PSYCHOLOGY LA English DT Article ID VICTIMIZATION; RAPE; FEAR; IMPACT; TRAUMA; STRESS C1 INDIANA UNIV PENN,INDIANA,PA 15701. NIMH,CTR STUDY VIOLENCE & ANTISOCIAL BEHAV,WASHINGTON,DC 20032. RP NORRIS, FH (reprint author), GEORGIA STATE UNIV,DEPT PSYCHOL,ATLANTA,GA 30303, USA. NR 58 TC 32 Z9 32 U1 0 U2 1 PU GUILFORD PUBLICATIONS INC PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0736-7236 J9 J SOC CLIN PSYCHOL JI J. Soc. Clin. Psychol. PD FAL PY 1991 VL 10 IS 3 BP 239 EP 261 DI 10.1521/jscp.1991.10.3.239 PG 23 WC Psychology, Clinical; Psychology, Social SC Psychology GA GU255 UT WOS:A1991GU25500001 ER PT J AU NEWLIN, DB PRETORIUS, MB AF NEWLIN, DB PRETORIUS, MB TI PRIOR EXPOSURES TO THE LABORATORY ENHANCE THE EFFECT OF ALCOHOL SO JOURNAL OF STUDIES ON ALCOHOL LA English DT Article AB We compared responses to 0.6 g/kg alcohol of normal male drinkers in a laboratory environment that was new to the subjects with the responses of a second group in the same environment who had two prior exposures to the laboratory environment and procedures. Alcohol-induced increases in heart rate, cheek temperature and self-reported intoxication were greater in the subjects who had previous exposure to the laboratory environment than in the subjects who had no such previous exposure. Differences in blood alcohol concentration (BAC) also were found between the two environments. However, differences in the physiological and self-report measures were found after matching subjects in terms of BAC. These results suggest that novelty effects may contaminate alcohol responses in novel laboratory environments, and they add to our understanding of nonpharmacological factors in the response to drugs. RP NEWLIN, DB (reprint author), NIDA,ADDICT RES CTR,BOX 5180,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. FU NIAAA NIH HHS [AA06433] NR 5 TC 20 Z9 20 U1 0 U2 0 PU ALCOHOL RES DOCUMENTATION INC CENT ALCOHOL STUD RUTGERS UNIV PI PISCATAWAY PA PO BOX 969, PISCATAWAY, NJ 08855-0969 SN 0096-882X J9 J STUD ALCOHOL JI J. Stud. Alcohol PD SEP PY 1991 VL 52 IS 5 BP 470 EP 473 PG 4 WC Substance Abuse; Psychology SC Substance Abuse; Psychology GA GD665 UT WOS:A1991GD66500012 PM 1943103 ER PT J AU STEWART, SFC NAST, EP ARABIA, FA TALBOT, TL PROSCHAN, M CLARK, RE AF STEWART, SFC NAST, EP ARABIA, FA TALBOT, TL PROSCHAN, M CLARK, RE TI ERRORS IN PRESSURE-GRADIENT MEASUREMENT BY CONTINUOUS WAVE DOPPLER ULTRASOUND - TYPE, SIZE AND AGE EFFECTS IN BIOPROSTHETIC AORTIC VALVES SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID PROSTHETIC VALVES; STENOSIS; INVITRO; ECHOCARDIOGRAPHY; OVERESTIMATION; SUBSTITUTES; POSITION; RECOVERY; FLOW AB The accuracy of continuous wave Doppler ultrasound in deriving pressure gradients across bioprosthetic heart valves was evaluated in an in vitro pulse duplicator. Simultaneous pressure transducer and Doppler measurements were made in new and explanted aortic bioprosthetic valves of several sizes and four types: Carpentier-Edwards, Ionescu-Shiley, Hancock standard and Hancock modified. The mean and peak gradients calculated by the modified Bernoulli equation from Doppler velocity measurements were always greater than those measured manometrically, despite corrections for location dependence of the manometric gradient (or pressure recovery). The relation between manometric and ultrasonically determined gradient was found to be statistically dependent on the valve type (mean gradient p < 0.0001; peak gradient p = 0.0003) and size (mean gradient p = 0.0089; peak gradient p = 0.0107). Effects of implantation were observed, but were not shown to be significant. It is concluded that the continuous wave Doppler velocity data overestimated prosthetic valve pressure gradient in all cases, even when pressure recovery was taken into account. Clinicians should be wary of Doppler data when making major diagnostic or therapeutic decisions. C1 NHLBI,BIOSTAT BRANCH,BETHESDA,MD 20892. NHLBI,CARDIAC SURG BRANCH,BETHESDA,MD 20892. NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 19 TC 23 Z9 24 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD SEP PY 1991 VL 18 IS 3 BP 769 EP 779 PG 11 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA GD037 UT WOS:A1991GD03700019 PM 1869741 ER PT J AU KRAGEL, AH GERTZ, SD ROBERTS, WC AF KRAGEL, AH GERTZ, SD ROBERTS, WC TI MORPHOLOGICAL COMPARISON OF FREQUENCY AND TYPES OF ACUTE LESIONS IN THE MAJOR EPICARDIAL CORONARY-ARTERIES IN UNSTABLE ANGINA-PECTORIS, SUDDEN CORONARY DEATH AND ACUTE MYOCARDIAL-INFARCTION SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID ANGIOGRAPHIC MORPHOLOGY; NECROPSY PATIENTS; THROMBOSIS; DISEASE; PLAQUES AB The frequency and type of acute lesions in the four major (right, left main, left anterior descending, left circumflex) epicardial coronary arteries were examined at necropsy in 14 patients with unstable angina pectoris, 21 patients with sudden coronary death and 32 patients with a fatal first acute myocardial infarction. None of the 67 patients had a grossly visible left ventricular scar (healed myocardial infarct) and only the group with acute myocardial infarction had left ventricular myocardial necrosis. Although the frequency of intraluminal thrombus was similar in patients with unstable angina (29%) and sudden death (29%) and significantly lower than in those with acute infarction (69%) (p = 0.02), the thrombus in the patients with unstable angina and sudden death consisted almost entirely of platelets and was nonocclusive, whereas the thrombus in the group with acute infarction consisted almost entirely of fibrin and was occlusive. The frequency of plaque rupture was insignificantly different in the groups with unstable angina (36%) and sudden death (19%), and was significantly lower than in the group with acute infarction (75%) (p = 0.02). The frequency of plaque hemorrhage was insignificantly different in the groups with unstable angina (64%) and sudden death (38%) and was significantly lower than in the group with acute infarction (90%) (p = 0.04). The frequency of atherosclerotic plaques containing multiluminal channels was similar in patients with unstable angina, sudden death and acute infarction (100%, 81% and 90%, respectively), but the percent of 5-mm long segments of the four major coronary arteries containing multiluminal channels (probably the result of organization of thrombus) was greatest in the unstable angina pectoris group (12% vs. 7% vs. 1%, respectively; p = 0.04). Thus, the frequency of thrombus, plaque rupture and plaque hemorrhage in the coronary arteries among patients with unstable angina pectoris and sudden coronary death was similar and significantly less than in the patients with acute myocardial infarction. The type of thrombus and the amount of lumen obstructed by thrombus were similar in the groups with unstable angina pectoris and sudden coronary death and quite different from those in the group with acute myocardial infarct group. C1 NHLBI,PATHOL BRANCH,BETHESDA,MD 20892. NR 16 TC 91 Z9 94 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD SEP PY 1991 VL 18 IS 3 BP 801 EP 808 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA GD037 UT WOS:A1991GD03700023 PM 1869744 ER PT J AU PARKER, CE IWAHASHI, H TOMER, KB AF PARKER, CE IWAHASHI, H TOMER, KB TI SPIN-TRAPPED RADICALS - DETERMINATION BY LC-TSP-MS AND LC-ESI-MS SO JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; SPRAY MASS-SPECTROMETRY; ELECTROSPRAY IONIZATION; METABOLISM; SEPARATION; INTERFACE; IDENTIFICATION; RESONANCE; PRESSURE; PROTEINS AB The 4-POBN[alpha-(4-pyridyl-1-oxide)-N-tert-butyl-nitrone] radical adducts of ethyl and pentyl radicals were determined by a combination of high performance liquid chromatography (HPLC) combined with electron paramagnetic resonance (EPR) with HPLC-electrospray (ESI)-mass spectrometry and HPLC-thermospray (TSP)-MS. The identification of the peak corresponding to the spin-trapped radical was done by performing HPLC-EPR under the same chromatographic conditions as the HPLC-MS. The radical adducts could be determined by both techniques, even though for ESI only 12-mu-L/min of the total 1 mL/min HPLC flow rate could be directed into the ion source. C1 NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709. RI Tomer, Kenneth/E-8018-2013 NR 34 TC 21 Z9 21 U1 0 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 1044-0305 J9 J AM SOC MASS SPECTR JI J. Am. Soc. Mass Spectrom. PD SEP-OCT PY 1991 VL 2 IS 5 BP 413 EP 418 DI 10.1016/1044-0305(91)85007-S PG 6 WC Chemistry, Analytical; Chemistry, Physical; Spectroscopy SC Chemistry; Spectroscopy GA GC827 UT WOS:A1991GC82700007 PM 24242692 ER PT J AU SZEMEREDI, K PACAK, K KOPIN, IJ GOLDSTEIN, DS AF SZEMEREDI, K PACAK, K KOPIN, IJ GOLDSTEIN, DS TI SYMPATHONEURAL AND SKELETAL-MUSCLE CONTRIBUTIONS TO PLASMA DOPA RESPONSES IN PITHED RATS SO JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM LA English DT Article DE DOPA; NOREPINEPHRINE; DIHYDROXYPHENYLGLYCOL; RAT; SYMPATHETIC NERVOUS SYSTEM; CURARE; CHLORISONDAMINE ID PHYSIOLOGICAL SIGNIFICANCE; CATECHOLS; 3,4-DIHYDROXYPHENYLALANINE; NOREPINEPHRINE AB Dihydroxyphenylalanine (DOPA) in plasma has been thought to originate from sympathetic nerve endings and to reflect catecholamine biosynthesis, because changes in DOPA levels follow pharmacologically- or environmentally-induced manipulations that alter turnover of the sympathetic neurotransmitter, norepinephrine (NE). Skeletal muscle may be an additional, non-neural source of circulating DOPA. In the present study we examined sympathoneuronal and skeletal muscle contributions to DOPA in arterial plasma in pithed rats. Electrical stimulation of the spinal cord causes discharges of sympathetic post-ganglionic neurons, with attendant release of NE into the bloodstream, and discharges of spinal motoneurons, which causes diffuse contraction of skeletal muscle. Stimulation of the spinal cord rapidly elevated arterial plasma concentrations of NE, dihydroxyphenylglycol (DHPG), and DOPA. Pre-treatment with curare, a skeletal muscle relaxant, did not affect the NE and DHPG responses but attenuated the DOPA responses by about 50%. Administration of chlorisondamine, a ganglionic blocker, abolished NE and DHPG responses to cord stimulation, and DOPA responses were decreased by about 90%. Adrenal-demudullation did not affect the stimulation-induced DOPA responses. The results demonstrate that in pithed rats undergoing spinal cord stimulation, DOPA is released into the bloodstream. Since this response is markedly inhibited after ganglionic blockade and also attenuated after skeletal muscle paralysis, the results provide indirect evidence that DOPA formed in sympathetic neurons can be stored in a non-neuronal pool and released during skeletal muscle contraction. C1 NINCDS,CLIN NEUROSCI BRANCH,BLDG 10,ROOM 8C103,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NHLBI,HYPERTENS ENDOCRINE BRANCH,BETHESDA,MD 20892. NR 17 TC 7 Z9 7 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-1838 J9 J AUTONOM NERV SYST JI J. Auton. Nerv. Syst. PD SEP PY 1991 VL 35 IS 3 BP 169 EP 174 DI 10.1016/0165-1838(91)90094-J PG 6 WC Neurosciences SC Neurosciences & Neurology GA GG753 UT WOS:A1991GG75300002 PM 1744376 ER PT J AU BRECHBIEL, MW PIPPIN, CG MCMURRY, TJ MILENIC, D ROSELLI, M COLCHER, D GANSOW, OA AF BRECHBIEL, MW PIPPIN, CG MCMURRY, TJ MILENIC, D ROSELLI, M COLCHER, D GANSOW, OA TI AN EFFECTIVE CHELATING AGENT FOR LABELING OF MONOCLONAL-ANTIBODY WITH BI-212 FOR ALPHA-PARTICLE MEDIATED RADIOIMMUNOTHERAPY SO JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS LA English DT Article ID TUMOR AB The ligand N[2-amino-3-(p-isothiocyanatophenyl)propyl]-(+/-)-trans-1,2-diaminocyclohexane-N,N',N''-pentaacetic acid has been synthesized and linked to IgG and to monoclonal antibody B72.3, and labelled with Bi-206 and Bi-212 to demonstrate the in vivo stability of the label and its utility for Bi-212-radioimmunotherapy. C1 NCI,RADIAT ONCOL BRANCH,INORGAN & RADIOIMMUNE CHEM SECT,BETHESDA,MD 20892. NCI,TUMOR IMMUNOL & BIOL LAB,BETHESDA,MD 20892. NR 16 TC 48 Z9 48 U1 1 U2 2 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK MILTON ROAD, CAMBRIDGE, CAMBS, ENGLAND CB4 4WF SN 0022-4936 J9 J CHEM SOC CHEM COMM JI J. Chem. Soc.-Chem. Commun. PD SEP 1 PY 1991 IS 17 BP 1169 EP 1170 DI 10.1039/c39910001169 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA GE516 UT WOS:A1991GE51600018 ER PT J AU HU, YL HIGHET, RJ MARION, D ZIFFER, H AF HU, YL HIGHET, RJ MARION, D ZIFFER, H TI MICROBIAL HYDROXYLATION OF A DIHYDROARTEMISININ DERIVATIVE SO JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS LA English DT Article ID QINGHAOSU ARTEMISININ; ANTIMALARIAL ACTIVITY; FALCIPARUM-MALARIA; CHLOROQUINE; TETRANDRINE; METABOLISM; CHEMISTRY; DRUG AB The fungus Beauveria sulfurescens has been employed to convert the 14-methyl group of the phenylcarbamoyl of dihydroartemisinin to the corresponding 14-hydroxymethyl derivative; the structure of the product was established by mass and 2D NMR spectroscopy. C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. CEN,RESONANCE MAGNET LAB,F-38041 GRENOBLE,FRANCE. NR 18 TC 8 Z9 8 U1 0 U2 1 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK MILTON ROAD, CAMBRIDGE, CAMBS, ENGLAND CB4 4WF SN 0022-4936 J9 J CHEM SOC CHEM COMM JI J. Chem. Soc.-Chem. Commun. PD SEP 1 PY 1991 IS 17 BP 1176 EP 1177 DI 10.1039/c39910001176 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA GE516 UT WOS:A1991GE51600022 ER PT J AU SPEAR, DJ KATZ, JL AF SPEAR, DJ KATZ, JL TI COCAINE AND FOOD AS REINFORCERS - EFFECTS OF REINFORCER MAGNITUDE AND RESPONSE REQUIREMENT UNDER 2ND-ORDER FIXED-RATIO AND PROGRESSIVE-RATIO SCHEDULES SO JOURNAL OF THE EXPERIMENTAL ANALYSIS OF BEHAVIOR LA English DT Article DE COCAINE; 2ND-ORDER SCHEDULES; PROGRESSIVE-RATIO SCHEDULES; SELF-ADMINISTRATION; REINFORCING EFFICACY; LEVER PRESS; SQUIRREL MONKEYS ID BEHAVIOR AB Reinforcer magnitude and fixed-ratio requirement were varied under two second-order schedules. Under one, the first sequence of a fixed number of responses completed after the lapse of a 10-min fixed interval produced reinforcement. Under the second, a second-order progressive-ratio schedule, the fixed number of responses increased after each reinforcement. Either cocaine (0 to 300-mu-g/kg/inj) or food (0 to 5,700 mg/delivery) reinforcers were delivered. Under some conditions, a 2-s illumination of stimulus lights occurred on completion of each ratio sequence. Under the second-order schedule, as cocaine dose or amount of food increased, rates of responding increased; at the highest values, rates of responding decreased. Increases in the ratio requirement from 10 to 170 responses minimally decreased overall response rates. Under the second-order progressive-ratio schedule, increases in dose of cocaine or amount of food increased rates of responding; at the highest amounts of food, rates of responding decreased but response rates at the highest dose of cocaine remained relatively high. The highest ratio requirement that was completed (breaking point) depended on the dose of cocaine but was less dependent on the amount of food. Removing brief-stimulus presentations had a greater effect on completion of ratio requirements with cocaine compared to food. C1 NIDA,RES CTR,LEXINGTON,KY 40583. RP SPEAR, DJ (reprint author), JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT & BEHAV SCI,720 RUTLAND AVE,BALTIMORE,MD 21205, USA. NR 29 TC 35 Z9 36 U1 0 U2 2 PU SOC EXP ANALYSIS BEHAVIOR INC PI BLOOMINGTON PA INDIANA UNIV DEPT PSYCHOLOGY, BLOOMINGTON, IN 47405 SN 0022-5002 J9 J EXP ANAL BEHAV JI J. Exp. Anal. Behav. PD SEP PY 1991 VL 56 IS 2 BP 261 EP 275 DI 10.1901/jeab.1991.56-261 PG 15 WC Psychology, Biological; Behavioral Sciences; Psychology, Experimental SC Psychology; Behavioral Sciences GA GG116 UT WOS:A1991GG11600009 PM 1955816 ER PT J AU GLOWA, JR JEFFREYS, RD RILEY, AL AF GLOWA, JR JEFFREYS, RD RILEY, AL TI DRUG DISCRIMINATION USING A CONDITIONED TASTE-AVERSION PARADIGM IN RHESUS-MONKEYS SO JOURNAL OF THE EXPERIMENTAL ANALYSIS OF BEHAVIOR LA English DT Article DE DRUG DISCRIMINATION; CONDITIONED TASTE AVERSION; ALPRAZOLAM; FIXED-RATIO SCHEDULE; LEVER PRESS; RHESUS MONKEYS ID BEHAVIORAL BASELINE; SQUIRREL-MONKEYS; STIMULUS; CHLORDIAZEPOXIDE; SUPPRESSION; ALPRAZOLAM AB The development of drug discrimination was assessed in rhesus monkeys using the conditioned taste-aversion paradigm. Monkeys were initially trained to respond under a fixed-ratio 30-response schedule of food-pellet delivery to assess the rate-decreasing effects of alprazolam (0.03 to 3 mg/kg, i.m., 60 min presession). Alprazolam decreased responding at doses greater than 0.1 mg/kg. Discriminative stimulus effects of alprazolam were then assessed by giving 0.03 mg/kg before sessions in which 1.8 mEq/kg lithium chloride was given immediately after the session (alprazolam/lithium session). On intervening days, saline was given before and after the session (saline/saline session). Rates of responding decreased over successive alprazolam/lithium sessions and also during the saline/saline session that immediately followed an alprazolam/lithium session. During subsequent saline/saline sessions, rates of responding returned to levels near baseline rates within two to four sessions. The discriminative stimulus effects of alprazolam were then assessed by giving 0.1 mg/kg before sessions in which 1 mg/kg d-amphetamine was given immediately after the session (alprazolam/d-amphetamine session). Rates of responding decreased during subsequent alprazolam/d-amphetamine sessions in drug-experienced monkeys, but did not decrease during intervening saline/saline sessions. These findings demonstrate that drug stimuli associated with postsession drug injections can rapidly develop control over behavior and suggest that similar methods be explored in the assessment of drug discrimination. C1 AMERICAN UNIV,WASHINGTON,DC 20016. RP GLOWA, JR (reprint author), NIMH,CLIN NEUROENDOCRINOL BRANCH,BIOPSYCHOL UNIT,BLDG 14D,RM 311,BETHESDA,MD 20892, USA. NR 22 TC 9 Z9 9 U1 0 U2 0 PU SOC EXP ANALYSIS BEHAVIOR INC PI BLOOMINGTON PA INDIANA UNIV DEPT PSYCHOLOGY, BLOOMINGTON, IN 47405 SN 0022-5002 J9 J EXP ANAL BEHAV JI J. Exp. Anal. Behav. PD SEP PY 1991 VL 56 IS 2 BP 303 EP 312 DI 10.1901/jeab.1991.56-303 PG 10 WC Psychology, Biological; Behavioral Sciences; Psychology, Experimental SC Psychology; Behavioral Sciences GA GG116 UT WOS:A1991GG11600012 PM 1659608 ER PT J AU BOERBOOM, LE OLINGER, GN RODRIGUEZ, ER FERRANS, VJ KISSEBAH, AH AF BOERBOOM, LE OLINGER, GN RODRIGUEZ, ER FERRANS, VJ KISSEBAH, AH TI ATHEROGENIC EFFECT OF BAROTRAUMA ON INSITU SAPHENOUS VEINS IN MONKEYS SO JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY LA English DT Article ID NONHUMAN PRIMATE MODEL; BYPASS GRAFTS; BIOCHEMICAL EVOLUTION; LIPID-ACCUMULATION; HISTOLOGIC FATE; DISTENSION; ASPIRIN; LIGHT AB The objectives of this study were to determine whether veins subjected to barotrauma in situ undergo lipid uptake and morphologic changes to the same extent as veins grafted into the arterial circulation. Saphenous veins in seven stump-tailed macaque monkeys were exposed bilaterally and were circumferentially dissected free from surrounding tissue only at isolated sites. Segments of the veins were distended for 1 minute at hydrostatic pressures of 125 or 350 mm Hg. An undistended segment served as control. A cephalic vein graft was interposed in the femoral artery for comparison with in situ veins. The animals were fed a diet that sustains plasma cholesterol levels of approximately 225 mg/dl. Saphenous veins and the cephalic vein grafts were explanted at 3 months for biochemical and histologic analyses. Cholesterol content in undistended saphenous veins was similar to that in veins distended at 125 or 350 mm Hg-105 +/- 15, 122 +/- 14, and 109 +/- 30-mu-g/100 mg wet tissue weight, respectively. Cholesterol content in cephalic vein grafts, 473 +/- 122-mu-g/100 mg, was greater (p < 0.001) than in saphenous veins at all distention pressures studied. There was no difference among the distention pressures in the intimal fraction of saphenous vein wall, with the pooled value being 20% +/- 12%. This contrasted with the value of 59% +/- 11% in cephalic vein grafts (p < 0.01). Endothelial coverage of the luminal surface in saphenous veins was similar among the levels of barotrauma, with the pooled value being 83% +/- 15%. Less of the lumen was covered with endothelium in cephalic vein grafts, 46% +/- 18% (p < 0.01). Slightly more medial fibrosis was observed in cephalic vein grafts as compared with saphenous veins (p < 0.05). These data demonstrate that barotrauma alone does not cause veins that remain in the venous system to undergo the lipid uptake or morphologic changes that occur in veins grafted into the arterial circulation in nonhuman primates. C1 MED COLL WISCONSIN,DEPT PHYSIOL,MILWAUKEE,WI 53226. MED COLL WISCONSIN,DEPT MED,MILWAUKEE,WI 53226. NHLBI,PATHOL BRANCH,BETHESDA,MD 20892. RP BOERBOOM, LE (reprint author), MED COLL WISCONSIN,MILWAUKEE REG MED CTR,DEPT CARDIOTHORAC SURG,8700 W WISCONSIN AVE,MILWAUKEE,WI 53226, USA. NR 18 TC 1 Z9 1 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0022-5223 J9 J THORAC CARDIOV SUR JI J. Thorac. Cardiovasc. Surg. PD SEP PY 1991 VL 102 IS 3 BP 448 EP 453 PG 6 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA GE660 UT WOS:A1991GE66000016 PM 1881183 ER PT J AU GARFINKEL, DJ HEDGE, AM YOUNGREN, SD COPELAND, TD AF GARFINKEL, DJ HEDGE, AM YOUNGREN, SD COPELAND, TD TI PROTEOLYTIC PROCESSING OF POL-TYB PROTEINS FROM THE YEAST RETROTRANSPOSON TY1 SO JOURNAL OF VIROLOGY LA English DT Article ID VIRUS-LIKE PARTICLES; COMPLETE NUCLEOTIDE-SEQUENCE; SACCHAROMYCES-CEREVISIAE; REVERSE-TRANSCRIPTASE; ELEMENT TRANSPOSITION; RETROVIRAL PROTEINS; SPT3 GENE; RNA; EXPRESSION; PROTEASE AB Using antibodies directed against the TYB1 protein of the transpositionally competent retrotransposon Ty1-H3, we have identified three mature proteins of 23, 60, and 90 kDa and processing intermediates of 140 and 160 kDa that are derived from the 190-kDa TYA1-TYB1 polyprotein. Mature proteins and variable amounts of the precursors cofractionate with Ty viruslike particles. The map locations and precursor-product relationships of mature TYB1 polypeptides suggest that p23 is Ty1 protease, p90 is integrase, and p60 contains reverse transcriptase and RNase H. Immunoprecipitation and immunoblot analyses of Ty1 proteins show that p190 is cleaved to form p160. The p160 intermediate is cleaved to form p23 and p140, and p140 is cleaved to form p90 and p60. Processing of TYB1 proteins is dependent on Ty1 protease. Immunoblot analysis of TYB proteins from different Ty1 isolates reveal that correct processing of TYB1 proteins is a characteristic of functional Ty1 elements, whereas aberrant processing is a common defect found in transposition-incompetent elements. RP GARFINKEL, DJ (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ABL,BASIC RES PROGRAM,POB B,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74101] NR 61 TC 58 Z9 59 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD SEP PY 1991 VL 65 IS 9 BP 4573 EP 4581 PG 9 WC Virology SC Virology GA GB018 UT WOS:A1991GB01800003 PM 1714514 ER PT J AU BLASCO, R COLE, NB MOSS, B AF BLASCO, R COLE, NB MOSS, B TI SEQUENCE-ANALYSIS, EXPRESSION, AND DELETION OF A VACCINIA VIRUS GENE ENCODING A HOMOLOG OF PROFILIN, A EUKARYOTIC ACTIN-BINDING PROTEIN SO JOURNAL OF VIROLOGY LA English DT Article ID CALF SPLEEN PROFILIN; AMINO-ACID SEQUENCE; ACANTHAMOEBA PROFILIN; NUCLEOTIDE-SEQUENCE; PHOSPHOLIPASE-C; DNA; INHIBITORS; RELEASE; CELLS; DEHYDROGENASE AB A 4,500-bp BamHI fragment, located within the HindIII A segment of the vaccinia virus genome, was found to contain eight potential coding regions for polypeptides of 78 to 346 amino acids. The open reading frames with 133, 346, and 125 codons were homologous to profilin (an actin-binding protein), 3-beta-hydroxysteroid dehydrogenase, and Cu-Zn superoxide dismutase, respectively. Sequence alignments indicated that the vaccinia virus and mammalian profilins were more closely related to each other than to known profilins of other eukaryotes. The expression and possible role of the prolifin homolog in the virus replicative cycle were therefore investigated. Antibody raised to Escherichia coli expressed vaccinia virus profilin was used to demonstrate the synthesis of the 15-kDa polypeptide at late times after vaccinia virus infection of mammalian cells. The protein accumulated in the cytoplasm, but only trace amounts remained associated with highly purified virions. The isolation of vaccinia virus mutants (in strains WR and IHD-J), with nearly the entire profilin gene replaced by the E. coli gpt gene, indicated that the protein is not essential for infectivity. The characteristic vaccinia virus-induced changes in actin fibers, seen by fluorescence microscopy, occurred in cells infected with the mutant. Moreover, the virus-encoded prolifin homolog was not required for actin-associated events, including intracellular virus movement to the periphery of the cell, formation of specialized microvilli, or release of mature virions, as shown by electron microscopy and yields of infectious intra- and extracellular virus. C1 NIAID,VIRAL DIS LAB,BETHESDA,MD 20892. RI Blasco, Rafael/B-5829-2016 NR 52 TC 74 Z9 83 U1 0 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD SEP PY 1991 VL 65 IS 9 BP 4598 EP 4608 PG 11 WC Virology SC Virology GA GB018 UT WOS:A1991GB01800006 PM 1870190 ER PT J AU SEDMAN, SA BARBOSA, MS VASS, WC HUBBERT, NL HAAS, JA LOWY, DR SCHILLER, JT AF SEDMAN, SA BARBOSA, MS VASS, WC HUBBERT, NL HAAS, JA LOWY, DR SCHILLER, JT TI THE FULL-LENGTH E6 PROTEIN OF HUMAN PAPILLOMAVIRUS TYPE-16 HAS TRANSFORMING AND TRANS-ACTIVATING ACTIVITIES AND COOPERATES WITH E7 TO IMMORTALIZE KERATINOCYTES IN CULTURE SO JOURNAL OF VIROLOGY LA English DT Article ID CARCINOMA CELL-LINES; TRANSCRIPTION; SUFFICIENT; DOMAINS; ENCODES; REGION; ZINC; GENE; RNA AB The wild-type E6 and E7 genes of human papillomavirus type 16 (HPV16) can cooperate to immortalize normal human keratinocytes in culture. The E6 open reading frame of HPV16 and other HPV types highly associated with cervical cancer has the potential of encoding both full-length E6 and two truncated E6* proteins, the latter being generated via splicing within the E6 open reading frame portion of the E6-E7 polycistronic transcript. Those types, such as HPV6, that are infrequently associated with cervical carcinoma lack the splice site and encode only a full-length E6. We have now found that, in addition to cooperating with E7 to immortalize keratinocytes, HPV16 E6 can induce anchorage-independent growth in NIH 3T3 cells and trans-activate the adenovirus E2 promoter. HPV6 E6 was also able to trans-activate the adenovirus E2 promoter, although it was inactive in both cell transformation assays. An HPV16 splice site mutant which expressed only the full-length HPV16 E6 was active in all three assays, indicating that the E6* proteins are not required for these activities. The plasmid which encodes the E6* proteins was inactive and did not potentiate the activity of the HPV16 splice site mutant. The mutation that prevented splicing in E6-E7 mRNA severely reduced the level of E7 protein and increased E6 protein. Taken together, the results suggest that the primary function of the splice within E6 is to facilitate the translation of E7 and reduce translation of full-length E6, rather than to generate biologically active E6* proteins. C1 NCI,CELLULAR ONCOL LAB,BETHESDA,MD 20892. FU NCI NIH HHS [TA-CA-B032] NR 26 TC 199 Z9 200 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD SEP PY 1991 VL 65 IS 9 BP 4860 EP 4866 PG 7 WC Virology SC Virology GA GB018 UT WOS:A1991GB01800037 PM 1651408 ER PT J AU EMERSON, SU MCRILL, C ROSENBLUM, B FEINSTONE, S PURCELL, RH AF EMERSON, SU MCRILL, C ROSENBLUM, B FEINSTONE, S PURCELL, RH TI MUTATIONS RESPONSIBLE FOR ADAPTATION OF HEPATITIS-A VIRUS TO EFFICIENT GROWTH IN CELL-CULTURE SO JOURNAL OF VIROLOGY LA English DT Article ID COMPLETE NUCLEOTIDE-SEQUENCE; VIRAL-RNA; POLIOVIRUS; PROTEINS; ASSOCIATION; REPLICATION; TRANSLATION; MUTANTS; REGION; CDNA AB Chimeric genomes of hepatitis A virus strain HM-175 were constructed from cDNA clones of the wild-type virus and its cell culture-adapted variant. RNA transcribed in vitro from each construct was assayed for infectivity by transfection of cultured cells. RNA transcribed from the wild-type cDNA clone was minimally infectious and produced virus that grew inefficiently in vitro, whereas that transcribed from certain chimeric genomes consistently produced virus that grew efficiently in cultured cells. Mutations in the P2 region were found to be necessary for efficient virus growth in vitro, while mutations in the 5' noncoding region imparted a conditional enhancement of growth in vitro. RP EMERSON, SU (reprint author), NIAID,INFECT DIS LAB,BETHESDA,MD 20892, USA. NR 14 TC 53 Z9 53 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD SEP PY 1991 VL 65 IS 9 BP 4882 EP 4886 PG 5 WC Virology SC Virology GA GB018 UT WOS:A1991GB01800040 PM 1651411 ER PT J AU COLMENARES, C SUTRAVE, P HUGHES, SH STAVNEZER, E AF COLMENARES, C SUTRAVE, P HUGHES, SH STAVNEZER, E TI ACTIVATION OF THE C-SKI ONCOGENE BY OVEREXPRESSION SO JOURNAL OF VIROLOGY LA English DT Article ID PROTO-ONCOGENE; MESSENGER-RNA; HIGHLY EFFICIENT; POINT MUTATION; SARCOMA VIRUS; MYB ONCOGENE; FOS PROTEIN; SEQUENCE; GENE; DNA AB The v-ski oncogene is a truncated version of the cellular proto-oncogene, c-ski, and lacks sequences coding for both the N- and C-terminal ends of the c-ski protein. In the region of overlap, v-ski and c-ski differ by only one amino acid. To determine whether these differences underlie v-ski's oncogenic activation, we have cloned cDNAs for several alternatively spliced c-ski mRNAs and introduced these cDNAs into replication-competent retroviral vectors. The biological activities of these c-ski constructs have been compared with those of v-ski. We found that all c-ski gene products, when expressed at high levels from the promoter in the retroviral long terminal repeat, can induce morphological transformation, anchorage independence, and muscle differentiation in avian cells. Cells that are susceptible to ski-induced transformation and myogenesis normally express endogenous c-ski at low levels. Thus, it appears that overexpression of ski is sufficient for oncogenic and myogenic activation. C1 UNIV CINCINNATI,MED CTR,DEPT MOLEC GENET BIOCHEM & MICROBIOL,CINCINNATI,OH 45267. NCI,FREDERICK CANC RES & DEV CTR,ABL,BASIC RES PROGRAM,FREDERICK,MD 21702. FU NCI NIH HHS [N01-CO-74101, R01-CA43600] NR 41 TC 85 Z9 87 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD SEP PY 1991 VL 65 IS 9 BP 4929 EP 4935 PG 7 WC Virology SC Virology GA GB018 UT WOS:A1991GB01800046 PM 1870207 ER PT J AU FLAMAND, L GOSSELIN, J DADDARIO, M HISCOTT, J ABLASHI, DV GALLO, RC MENEZES, J AF FLAMAND, L GOSSELIN, J DADDARIO, M HISCOTT, J ABLASHI, DV GALLO, RC MENEZES, J TI HUMAN HERPESVIRUS-6 INDUCES INTERLEUKIN-1-BETA AND TUMOR-NECROSIS-FACTOR-ALPHA, BUT NOT INTERLEUKIN-6, IN PERIPHERAL-BLOOD MONONUCLEAR CELL-CULTURES SO JOURNAL OF VIROLOGY LA English DT Note ID HUMAN-IMMUNODEFICIENCY-VIRUS; HBLV HUMAN HERPESVIRUS-6; B-LYMPHOTROPIC VIRUS; EXANTHEM SUBITUM; SYNOVIAL-FLUID; HHV-6; EXPRESSION; ANTIBODY; SEROEPIDEMIOLOGY; REPLICATION AB The human herpesvirus 6 (HHV-6) is known to interact intimately with cells of the immune system. Here we report that HHV-6 is a potent inducer of interleukin-1-beta (IL-1-beta) and tumor necrosis factor alpha (TNF-alpha) in cultures of peripheral blood mononuclear cells. In contradistinction, HHV-6 has no effect on IL-6 synthesis. Maximal IL-1-beta and TNF-alpha-gene transcription, as detected by polymerase chain reaction amplification analysis, is observed at 12 and 6 h postinfection, respectively. Release of IL-1-beta and TNF-alpha-into the culture supernatants peaked at 24 h and gradually decreased with time. Heat-inactivated virus was unable to stimulate IL-1-beta and TNF-alpha-syntheses, whereas UV-irradiated virus retained the full monokine-inducing potential of the native particle. Preincubation of viral preparation with neutralizing anti-HHV-6 antibody resulted in the abrogation of this cytokine-inducing effect, whereas treatment of cells with phosphonoacetic acid (an inhibitor of viral DNA polymerase activity) had no effect on the ability of the virus to stimulate monokine release. These results indicate that HHV-6 can exert a strong immunomodulatory effect by stimulating the cells of myeloid lineage to produce these cytokines. C1 UNIV MONTREAL,FAC MED,PEDIAT RES CTR,IMMUNOVIROL LAB,3175 ST CATHERINE RD,MONTREAL H3C 3J7,QUEBEC,CANADA. LADY DAVIS INST MED RES,MONTREAL H3T 1E2,QUEBEC,CANADA. NCI,BETHESDA,MD 20892. HOP ST JUSTINE,MONTREAL H3T 1C5,QUEBEC,CANADA. NR 35 TC 167 Z9 169 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD SEP PY 1991 VL 65 IS 9 BP 5105 EP 5110 PG 6 WC Virology SC Virology GA GB018 UT WOS:A1991GB01800071 PM 1651426 ER PT J AU HARRIS, T LIPSITZ, LA KLEINMAN, JC CORNONIHUNTLEY, J AF HARRIS, T LIPSITZ, LA KLEINMAN, JC CORNONIHUNTLEY, J TI POSTURAL CHANGE IN BLOOD-PRESSURE ASSOCIATED WITH AGE AND SYSTOLIC BLOOD-PRESSURE - THE NATIONAL-HEALTH AND NUTRITION EXAMINATION SURVEY .2. SO JOURNALS OF GERONTOLOGY LA English DT Article ID ORTHOSTATIC HYPOTENSION; OLD-AGE AB The prevalence of postural change in blood pressure and its association with age and systolic blood pressure were examined in data from 8,574 White nondiabetic persons aged 25-74 who participated in the second National Health and Nutrition Examination Survey (1976-1980). Postural change in blood pressure was defined as a drop of 20 mm Hg or more on change from supine to seated position. In subjects on no antihypertensive medications (n = 7,316), the prevalence of postural change in blood pressure increased with older age and with higher blood pressure levels, regardless of age. However, systolic blood pressure levels also increased with age. In logistic regression models, level of supine systolic blood pressure was strongly related to postural change in blood pressure (Relative odds (RO) = 1.59, 95% confidence interval (CI) = 1.49, 1.70 for a 10 mm Hg increase in systolic blood pressure) whereas age was not related to postural change in blood pressure (RO for age = 1.07, CI = .89, 1.19 for a 10-year increase in age). Results were similar for those medicated for hypertension. All results were unchanged by addition of health status indicators, including report of hospitalization and number of medical conditions, to the model. These data suggest that the age-related increase in the prevalence of postural hypotension previously reported may be partially explained by age-associated increases in systolic blood pressure. C1 BETH ISRAEL HOSP,BOSTON,MA 02215. BRIGHAM & WOMENS HOSP,BOSTON,MA 02115. HEBREW REHAB CTR AGING,BOSTON,MA. NIA,BETHESDA,MD 20892. HARVARD UNIV,DIV AGING,CAMBRIDGE,MA 02138. RP HARRIS, T (reprint author), NATL CTR HLTH STAT,6525 BELCREST RD,HYATTSVILLE,MD 20782, USA. FU NIA NIH HHS [AG-04390, AG-06443] NR 24 TC 44 Z9 45 U1 0 U2 1 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 0022-1422 J9 J GERONTOL JI J. Gerontol. PD SEP PY 1991 VL 46 IS 5 BP M159 EP M163 PG 5 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA GE787 UT WOS:A1991GE78700002 PM 1890281 ER PT J AU SACCHI, N AF SACCHI, N TI LEUKEMIA IN DOWNS-SYNDROME SO LEUKEMIA LA English DT Article; Proceedings Paper CT INTERNATIONAL RESEARCH SYMP ON AETIOLOGY OF LEUKAEMIA CY MAR 11, 1991 CL LONDON, ENGLAND SP LEUKAEMIA RES FUND ID TRANSIENT MYELOPROLIFERATIVE DISORDER; ACUTE-LEUKEMIA; TRISOMY-21 RP SACCHI, N (reprint author), NCI,MOLEC ONCOL LAB,FREDERICK,MD 21701, USA. NR 19 TC 2 Z9 2 U1 0 U2 1 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0887-6924 J9 LEUKEMIA JI Leukemia PD SEP PY 1991 VL 5 IS 9 BP 822 EP 823 PG 2 WC Oncology; Hematology SC Oncology; Hematology GA GQ771 UT WOS:A1991GQ77100017 PM 1834895 ER PT J AU CLEVIDENCE, BA BALLARDBARBASH, R AF CLEVIDENCE, BA BALLARDBARBASH, R TI TOCOPHEROL CONTENTS OF LIPOPROTEINS FROM FROZEN PLASMA SEPARATED BY AFFINITY-CHROMATOGRAPHY SO LIPIDS LA English DT Article ID LOW-DENSITY-LIPOPROTEIN; ALPHA-TOCOPHEROL; VITAMIN-E; LIQUID-CHROMATOGRAPHY; ENDOTHELIAL-CELLS; GAMMA-TOCOPHEROL; CYTO-TOXICITY; OXIDATION; STORAGE; SERUM AB We investigated whether freezing and storage of plasma altered alpha-tocopherol levels of whole plasma or the lipoprotein fractions derived from such plasma. Plasma from 24 men, at each of two collection periods, was frozen at -20-degrees-C for six weeks, then high-density lipoproteins (HDL) were separated from low- plus very low-density lipoproteins (LDL-VLDL) by heparin affinity chromatography. Whole plasma and the lipoprotein fractions were analyzed for alpha-tocopherol content and compared to counterparts from fresh plasma. Freezing and storage did not reduce alpha-tocopherol levels of plasma or the lipoprotein fractions. alpha-Tocopherol values from fresh and frozen plasma were highly correlated for both plasma (period 1, r = 0.94; period 2, r = 0.93) and the LDL-VLDL fractions (periods 1 and 2, r = 0.97). Percent distribution of alpha-tocopherol between the two lipoprotein fractions was comparable for lipoproteins derived from fresh and frozen plasma. Under the storage conditions used in this study, plasma can be frozen for at least six weeks prior to lipoprotein fractionation with no detectable detrimental effects on alpha-tocopherol content of either plasma or lipoproteins. C1 US DEPT HHS,NCI,DIV CANC PREVENT & CONTROL,BETHESDA,MD 20892. RP CLEVIDENCE, BA (reprint author), USDA ARS,BELTSVILLE AGR RES CTR,BELTSVILLE HUMAN NUTR RES CTR,LIPID NUTR LAB,BLDG 308,RM 115,BELTSVILLE,MD 20705, USA. NR 27 TC 11 Z9 11 U1 0 U2 2 PU AMER OIL CHEMISTS SOC PI CHAMPAIGN PA 1608 BROADMOOR DRIVE, CHAMPAIGN, IL 61821-0489 SN 0024-4201 J9 LIPIDS JI Lipids PD SEP PY 1991 VL 26 IS 9 BP 723 EP 728 DI 10.1007/BF02535621 PG 6 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA GG748 UT WOS:A1991GG74800009 PM 1762518 ER PT J AU SCHOOLMAN, HM AF SCHOOLMAN, HM TI OBLIGATIONS OF THE EXPERT SYSTEM BUILDER - MEETING THE NEEDS OF THE USER SO M D COMPUTING LA English DT Article DE EXPERT SYSTEMS; MEDICAL DEVICES; FDA REGULATION; SENSITIVITY; SPECIFICITY; ROBUSTNESS ID MEDICINE AB Builders of expert systems have generally accepted the principle that computer software should not be subject to government regulation if health care practitioners can be expected to interpret and apply the systems intelligently. The purpose of this paper is to identify the information that builders must make available to permit health care practitioners to exercise their clinical judgment in interpreting and applying the output of computing systems. RP SCHOOLMAN, HM (reprint author), NATL LIB MED,8600 ROCKVILLE PIKE,BETHESDA,MD 20894, USA. NR 6 TC 7 Z9 7 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0724-6811 J9 M D COMPUT JI M D Comput. PD SEP-OCT PY 1991 VL 8 IS 5 BP 316 EP 321 PG 6 WC Computer Science, Interdisciplinary Applications; Medical Informatics SC Computer Science; Medical Informatics GA GM192 UT WOS:A1991GM19200008 ER PT J AU WAN, XM FU, TC SMITH, PH BRAINARD, JR LONDON, RE AF WAN, XM FU, TC SMITH, PH BRAINARD, JR LONDON, RE TI MAGNETIC-RESONANCE-IMAGING STUDY OF THE RAT CEREBRAL VENTRICULAR SYSTEM UTILIZING INTRACEREBRALLY ADMINISTERED CONTRAST AGENTS SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article ID SUBCELLULAR-LOCALIZATION; MR CISTERNOGRAPHY; BRAIN-TUMORS; SIALIC-ACID; GADOLINIUM C1 NATL TAIWAN UNIV,COLL MED,DEPT PHYSIOL,TAIPEI 10018,TAIWAN. UNIV CALIF LOS ALAMOS SCI LAB,INC-4,LOS ALAMOS,NM 87545. RP WAN, XM (reprint author), NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 28 TC 17 Z9 18 U1 0 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0740-3194 J9 MAGNET RESON MED JI Magn.Reson.Med. PD SEP PY 1991 VL 21 IS 1 BP 97 EP 106 DI 10.1002/mrm.1910210112 PG 10 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA GD987 UT WOS:A1991GD98700010 PM 1943681 ER PT J AU CANNON, RO AF CANNON, RO TI MICROVASCULAR ANGINA - CARDIOVASCULAR INVESTIGATIONS REGARDING PATHOPHYSIOLOGY AND MANAGEMENT SO MEDICAL CLINICS OF NORTH AMERICA LA English DT Article ID NORMAL CORONARY-ARTERIES; ABNORMAL VASODILATOR RESERVE; SYNDROME-X; CHEST PAIN; FLOW RESERVE; FOLLOW-UP; PSYCHIATRIC-ILLNESS; PANIC DISORDER; PECTORIS; ARTERIOGRAMS C1 NHLBI,CARDIOL BRANCH,CARDIOVASC DIAG SECT,BETHESDA,MD 20892. NR 76 TC 24 Z9 25 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0025-7125 J9 MED CLIN N AM JI Med. Clin. N. Am. PD SEP PY 1991 VL 75 IS 5 BP 1097 EP 1118 PG 22 WC Medicine, General & Internal SC General & Internal Medicine GA GH330 UT WOS:A1991GH33000005 PM 1895808 ER PT J AU RICHTER, JE BEITMAN, BD CANNON, RO AF RICHTER, JE BEITMAN, BD CANNON, RO TI UNEXPLAINED CHEST PAIN - DIFFERENTIAL-DIAGNOSIS OF CHEST PAIN WITH NORMAL CARDIAC STUDIES - PREFACE SO MEDICAL CLINICS OF NORTH AMERICA LA English DT Editorial Material C1 UNIV MISSOURI,HOSP & CLIN,PSYCHIAT CLIN,COLUMBIA,MO 65212. NIH,BETHESDA,MD 20892. RP RICHTER, JE (reprint author), DIV GASTROENTEROL,UAB STN,ZIEGLER BLDG,ROOM 635,BIRMINGHAM,AL 35294, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0025-7125 J9 MED CLIN N AM JI Med. Clin. N. Am. PD SEP PY 1991 VL 75 IS 5 BP R10 EP R11 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA GH330 UT WOS:A1991GH33000001 ER PT J AU MCKENZIE, R TRAVIS, WD DOLAN, SA PITTALUGA, S FEUERSTEIN, IM SHELHAMER, J YARCHOAN, R MASUR, H AF MCKENZIE, R TRAVIS, WD DOLAN, SA PITTALUGA, S FEUERSTEIN, IM SHELHAMER, J YARCHOAN, R MASUR, H TI THE CAUSES OF DEATH IN PATIENTS WITH HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION - A CLINICAL AND PATHOLOGICAL-STUDY WITH EMPHASIS ON THE ROLE OF PULMONARY-DISEASES SO MEDICINE LA English DT Review ID PNEUMOCYSTIS-CARINII PNEUMONIA; IMMUNE-DEFICIENCY-SYNDROME; PLACEBO-CONTROLLED TRIAL; NONSPECIFIC INTERSTITIAL PNEUMONITIS; AVIUM-INTRACELLULARE INFECTION; LEUKOCYTE-A INTERFERON; AIDS-RELATED COMPLEX; ACQUIRED-IMMUNODEFICIENCY; KAPOSIS SARCOMA; CYTOMEGALO-VIRUS C1 NIAID,CTR CLIN,BETHESDA,MD 20892. NCI,BETHESDA,MD 20892. NR 117 TC 129 Z9 133 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0025-7974 J9 MEDICINE JI Medicine (Baltimore) PD SEP PY 1991 VL 70 IS 5 BP 326 EP 343 DI 10.1097/00005792-199109000-00004 PG 18 WC Medicine, General & Internal SC General & Internal Medicine GA GG807 UT WOS:A1991GG80700004 PM 1656164 ER PT J AU WEISS, LM GAFFEY, MJ WARHOL, MJ MEHTA, P BONSIB, SM BRUDER, E SANTOS, E MEDERIOS, LJ AF WEISS, LM GAFFEY, MJ WARHOL, MJ MEHTA, P BONSIB, SM BRUDER, E SANTOS, E MEDERIOS, LJ TI IMMUNOCYTOCHEMICAL CHARACTERIZATION OF A MONOCLONAL-ANTIBODY DIRECTED AGAINST MITOCHONDRIA REACTIVE IN PARAFFIN-EMBEDDED SECTIONS SO MODERN PATHOLOGY LA English DT Article DE IMMUNOHISTOCHEMISTRY; PARAFFIN SECTIONS; MONOCLONAL ANTIBODIES; MES-13; IMMUNOELECTRON MICROSCOPY ID H-RAS P21; CELL-CARCINOMA; DEFICIENCY; ADENOMAS; PROTEINS; TISSUES AB The monoclonal antibody mES 13 was previously produced against bacterially expressed BALB ras p21 and was reported to have both membrane and cytoplasmic reactivity in formalin-fixed, paraffin-embedded tissue sections. In the current study, the cytoplasmic reactivity of mES 13 is investigated and demonstrated to be mitochondrial. Immunoelectron microscopic studies showed specific labeling of mitochondria without labeling of other organelles. In normal tissues, the antibody strongly labeled tissues known to have large amounts of mitochondria such as renal tubules, hepatocytes, and myocardium. The pattern of reactivity of tumors generally mimicked that of normal tissues, with carcinomas and melanomas usually showing stronger staining than sarcomas and lymphomas. Two granular cell tumors were negative. Among renal neoplasms, mES 13 strongly labeled renal oncocytomas and granular cell renal cell carcinomas and showed weaker staining of clear cell and chromophobe cell tumors. The mES 13 antibody should be useful in the characterization and diagnosis of tumors in which oncocytoma is in the differential diagnosis, especially when only paraffin-embedded tissue is available for study. C1 PENN HOSP,PHILADELPHIA,PA 19107. UNIV IOWA HOSP & CLIN,IOWA CITY,IA 52242. NIH,BETHESDA,MD 20892. RP WEISS, LM (reprint author), CITY HOPE NATL MED CTR,DEPT PATHOL,DUARTE,CA 91010, USA. FU NCI NIH HHS [CA 37194] NR 22 TC 24 Z9 24 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD SEP PY 1991 VL 4 IS 5 BP 596 EP 601 PG 6 WC Pathology SC Pathology GA GF729 UT WOS:A1991GF72900009 PM 1758871 ER PT J AU SATCHIDANANDAM, V ZAVALA, F MOSS, B AF SATCHIDANANDAM, V ZAVALA, F MOSS, B TI STUDIES USING A RECOMBINANT VACCINIA VIRUS EXPRESSING THE CIRCUMSPOROZOITE PROTEIN OF PLASMODIUM-BERGHEI SO MOLECULAR AND BIOCHEMICAL PARASITOLOGY LA English DT Article DE PLASMODIUM-BERGHEI; CIRCUMSPOROZOITE PROTEIN; VACCINIA VIRUS; HUMORAL IMMUNITY; CELL-MEDIATED IMMUNITY ID PROTECTIVE ANTIGEN; MURINE MALARIA; SPOROZOITES; GENE; FALCIPARUM; IMMUNIZATION; GLYCOPROTEIN; ANTIBODIES; INFECTION; RESPONSES AB A recombinant vaccinia virus was constructed which expressed the circumsporozoite protein of Plasmodium berghei. Four different strains of mice belonging to different haplotypes were immunized with the recombinant virus. The antibody response to the circumsporozoite protein as well as to vaccinia virus varied among the strains, independently of each other. The anti-circumsporozoite protein titers were comparable to that obtained on immunization with irradiated sporozoites. Spleen cells from H2d mice immunized with P. berghei sporozoites showed a significant proliferative response when cultured in vitro with a low multiplicity of the recombinant vaccinia virus. A weak cytotoxic T lymphocyte response specifically targeting the circumsporozoite protein could be identified in spleens of BALB/c (H2d) mice immunized with vaccinia virus when BALB 3T3 cells transformed with a plasmid expressing the circumsporozoite protein under control of the simian virus 40 promoter were used as target cells in the cytotoxic T lymphocyte assay. However, none of the recombinant virus-immunized animals could be protected from a challenge of sporozoites even at the lowest dose of parasite used. C1 NIAID,VIRAL DIS LAB,BETHESDA,MD 20892. NYU,SCH MED,DEPT MED & MOLEC PARISITOL,NEW YORK,NY 10003. NR 32 TC 18 Z9 18 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-6851 J9 MOL BIOCHEM PARASIT JI Mol. Biochem. Parasitol. PD SEP PY 1991 VL 48 IS 1 BP 89 EP 100 DI 10.1016/0166-6851(91)90167-5 PG 12 WC Biochemistry & Molecular Biology; Parasitology SC Biochemistry & Molecular Biology; Parasitology GA GB991 UT WOS:A1991GB99100010 PM 1779992 ER PT J AU DUBIN, RA GOPALSRIVASTAVA, R WAWROUSEK, EF PIATIGORSKY, J AF DUBIN, RA GOPALSRIVASTAVA, R WAWROUSEK, EF PIATIGORSKY, J TI EXPRESSION OF THE MURINE ALPHA-B-CRYSTALLIN GENE IN LENS AND SKELETAL-MUSCLE - IDENTIFICATION OF A MUSCLE-PREFERRED ENHANCER SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID HEAT-SHOCK PROTEINS; NON-LENTICULAR TISSUES; CREATINE-KINASE GENE; CHLORAMPHENICOL ACETYLTRANSFERASE; REGULATORY ELEMENTS; TRANSGENIC MICE; CELL-LINES; WHITE GENE; DROSOPHILA; SEQUENCE AB The alpha-B-crystallin gene is expressed at high levels in lens and at lower levels in some other tissues, notably skeletal and cardiac muscle, kidney, lung, and brain. A promoter fragment of the murine alpha-B-crystallin gene extending from positions -661 to + 44 and linked to the bacterial chloramphenicol acetyltransferase (CAT) gene showed preferential expression in lens and skeletal muscle in transgenic mice. Transfection experiments revealed that a region between positions -426 and -257 is absolutely required for expression in C2C12 and G8 myotubes, while sequences downstream from position - 115 appear to be determinants for lens expression. In association with a heterologous promoter, a -427 to -259 fragment functions as a strong enhancer in C2C12 myotubes and less efficiently in myoblasts and lens. Gel shift and methylation interference studies demonstrated that nuclear proteins from C2C12 myoblasts and myotubes specifically bind to the enhancer. C1 NEI,MOLEC & DEV BIOL LAB,BETHESDA,MD 20892. SMITHKLINE BEECHAM,RES & DEV,KING OF PRUSSIA,PA 19406. RI Wawrousek, Eric/A-4547-2008 NR 53 TC 56 Z9 56 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD SEP PY 1991 VL 11 IS 9 BP 4340 EP 4349 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA GC396 UT WOS:A1991GC39600007 PM 1875925 ER PT J AU LANDSMAN, D BUSTIN, M AF LANDSMAN, D BUSTIN, M TI ASSESSMENT OF THE TRANSCRIPTIONAL ACTIVATION POTENTIAL OF THE HMG CHROMOSOMAL-PROTEINS SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID MOBILITY-GROUP PROTEINS; HUMAN CDNA SEQUENCE; DNA-BINDING DOMAIN; ACTIVE CHROMATIN; SECONDARY-STRUCTURE; TRANSCRIBED GENES; NUCLEAR PROTEINS; MULTIGENE FAMILY; YEAST; NUCLEOSOMES AB Chromosomal proteins HMG-14, HMG-17, and HMG-1 are among the most abundant, ubiquitous, and evolutionarily conserved nonhistone proteins. Analysis of their structure reveals features which are similar to those of certain transcription factors. The distribution of charged amino acid residues along the polypeptide chains is asymmetric: positive charges are clustered toward the N-terminal region, while negative charges are clustered toward the C-terminal region. The residues in the C-terminal region have the potential to form alpha helices with negatively charged surfaces. The abilities of HMG-14, -17, and -1 to function as transcriptional activators were studied in Saccharomyces cerevisiae cells expressing LexA-HMG fusion proteins (human HMG-14 and -17 and rat HMG-1) which bind to reporter molecules containing the beta-galactosidase gene downstream from a lexA operator. Fusion constructs expressing deletion mutants of HMG-14, -17, and -1 were also tested. Analysis of binding to the lexA operator with in vitro-synthesized fusion proteins shows that there are more sites for HMG-14, -17, and -1 binding than for LexA binding and that only the fusion constructs which contain the C-terminal, acidic domains of HMG-17 bind the lexA operator specifically. None of the LexA-HMG fusion protein constructs elevate the level of beta-galactosidase activity in transfected yeast cells. Thus, although HMG-14, -17, and -1 are structurally similar to acidic transcriptional activators, these chromosomal proteins do not function as activators in this test system. C1 NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. RP LANDSMAN, D (reprint author), NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BLDG 38A,ROOM 8N-807,BETHESDA,MD 20894, USA. RI Landsman, David/C-5923-2009; Bustin, Michael/G-6155-2015; OI Landsman, David/0000-0002-9819-6675 NR 53 TC 43 Z9 43 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD SEP PY 1991 VL 11 IS 9 BP 4483 EP 4489 PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA GC396 UT WOS:A1991GC39600024 PM 1908554 ER PT J AU SHIRAKATA, M HUPPI, K USUDA, S OKAZAKI, K YOSHIDA, K SAKANO, H AF SHIRAKATA, M HUPPI, K USUDA, S OKAZAKI, K YOSHIDA, K SAKANO, H TI HMG1-RELATED DNA-BINDING PROTEIN ISOLATED WITH V-(D)-J RECOMBINATION SIGNAL PROBES SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID IMMUNOGLOBULIN GENE REARRANGEMENT; COMBINED IMMUNE-DEFICIENCY; SEX-DETERMINING REGION; HEAVY-CHAIN GENES; V(D)J RECOMBINATION; SOMATIC RECOMBINATION; MOLECULAR-GENETICS; SPECIFICALLY BINDS; NUCLEAR-PROTEIN; SEQUENCE AB In order to isolate cDNA clones for DNA-binding components of the V-(D)-J recombinase, phage libraries from a pre-B-cell line were screened with a radiolabeled probe containing recombination signal sequences (RSS). Among prospective clones, cDNA T160 was analyzed further. It produced a protein of 80.6 kDa which bound to DNA containing RSS but not to DNA in which the RSS had been mutated. A search of a data base revealed that the T160 protein has significant sequence homology (56%) to the nonhistone chromosomal protein HMG1 within the C-terminal region of 80 amino acids. DNA-binding analysis with truncated proteins showed that the HMG homology region is responsible for DNA binding. Using restriction fragment length polymorphisms, the T160 gene was mapped at the proximal end of mouse chromosome 2. Evidence was obtained for genetic linkage between the T160 gene and the recombination activator genes RAG-1 and RAG-2. C1 UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,DIV IMMUNOL,BERKELEY,CA 94720. INST PHYS & CHEM RES,TSUKUBA CTR LIFE SCI,DIV GENE REGULAT,TSUKUBA 305,JAPAN. NCI,GENET LAB,BETHESDA,MD 20892. RI Okazaki, Kenji/H-4393-2012 FU NIAID NIH HHS [AI-18790] NR 55 TC 91 Z9 92 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD SEP PY 1991 VL 11 IS 9 BP 4528 EP 4536 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA GC396 UT WOS:A1991GC39600029 PM 1678855 ER PT J AU GREEN, JE AF GREEN, JE TI TRANS ACTIVATION OF NERVE GROWTH-FACTOR IN TRANSGENIC MICE CONTAINING THE HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-I TAX GENE SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID TROPICAL SPASTIC PARAPARESIS; COLONY-STIMULATING FACTOR; LONG TERMINAL REPEAT; LEUKEMIA-VIRUS; TRANSCRIPTIONAL ACTIVATOR; INTERLEUKIN-2 RECEPTOR; CYCLIC-AMP; HTLV-I; FUNCTIONAL-ANALYSIS; P40X PROTEIN AB Three lines of transgenic mice containing the human T-cell lymphotropic virus type I (HTLV-I) tax gene develop neurofibromas composed of perineural fibroblasts (S. H. Hinrichs, M. Nerenberg, R. K. Reynolds, G. Khoury, and G. Jay, Science 237:1340-1343, 1987; M. Nerenberg, S. H. Hinrichs, R. K. Reynolds, G. Khoury, and G. Jay, Science 237:1324-1327, 1987). Tumors and tumor cell lines derived from these mice produce neurite outgrowth from PC-12 cells and nerve growth factor (NGF), as determined by RNA (Northern) blot analysis and enzyme-linked immunosorbent assays. In vitro cotransfection studies demonstrate that Tax is able to trans activate the NGF promoter in NIH 3T3 fibroblast cells. The major cis-acting tax-responsive element in the NGF promoter (AGGGTGTGACGA) has 92% homology with a tax-responsive element contained within the 21-bp repeats of the HTLV-I long terminal repeat. The receptor for NGF is also expressed in the transgenic tumor cells, suggesting that Tax may activate an autocrine mechanism through the upregulation of NGF. RP GREEN, JE (reprint author), NCI,MOLEC ONCOL LAB,FREDERICK,MD 21702, USA. NR 51 TC 30 Z9 30 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD SEP PY 1991 VL 11 IS 9 BP 4635 EP 4641 PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA GC396 UT WOS:A1991GC39600040 PM 1875943 ER PT J AU UHL, GR WALTHER, D NISHIMORI, T BUZZI, MG MOSKOWITZ, MA AF UHL, GR WALTHER, D NISHIMORI, T BUZZI, MG MOSKOWITZ, MA TI JUN-B, C-JUN, JUN-D AND C-FOS MESSENGER-RNAS IN NUCLEUS-CAUDALIS NEURONS - RAPID SELECTIVE ENHANCEMENT BY AFFERENT STIMULATION SO MOLECULAR BRAIN RESEARCH LA English DT Article DE JUN-B; C-JUN; JUN-D; TRANSCRIPTION FACTOR; GENE REGULATION; PREPROENKEPHALIN ID RAT SPINAL-CORD; MESSENGER-RNA; TRANSCRIPTIONAL REGULATION; INSITU HYBRIDIZATION; GENE-EXPRESSION; GROWTH-FACTORS; CYCLIC-AMP; PREPROENKEPHALIN; PROTEIN; PROENKEPHALIN AB In situ hybridization using cDNAs complementary to specific regions of the mRNAs encoding 3 members of the jun transcription factor gene family and c-fos reveals modest levels of hybridization over superficial laminae of the nucleus caudalis of the spinal tract of the trigeminal in sections taken from unstimulated brains. Jun B expression is markedly and rapidly enhanced ipsilateral to electrical stimulation of the trigeminal ganglion. C-fos mRNA levels also show changes, especially after higher intensity stimulation. Smaller alterations in c-jun (jun A) and jun D do not reach statistical significance. In each instance of altered expression, more neurons express hybridization densities above background levels after stimulation. Parallels between these alterations and changes in the expression of preproenkephalin in these same neuronal populations are discussed. C1 JOHNS HOPKINS UNIV,DEPT NEUROL,BALTIMORE,MD 21218. JOHNS HOPKINS UNIV,DEPT NEUROSCI,BALTIMORE,MD 21218. MASSACHUSETTS GEN HOSP,DEPT NEUROL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT NEUROSCI,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02115. RP UHL, GR (reprint author), NIDA,ARC,MOLEC NEUROBIOL LAB,BOX 5180,BALTIMORE,MD 21224, USA. RI Moskowitz, Michael/D-9916-2011 NR 28 TC 33 Z9 33 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD SEP PY 1991 VL 11 IS 2 BP 133 EP 141 DI 10.1016/0169-328X(91)90115-E PG 9 WC Neurosciences SC Neurosciences & Neurology GA GF883 UT WOS:A1991GF88300005 PM 1661810 ER PT J AU YU, L NGUYEN, H LE, H BLOEM, LJ KOZAK, CA HOFFMAN, BJ SNUTCH, TP LESTER, HA DAVIDSON, N LUBBERT, H AF YU, L NGUYEN, H LE, H BLOEM, LJ KOZAK, CA HOFFMAN, BJ SNUTCH, TP LESTER, HA DAVIDSON, N LUBBERT, H TI THE MOUSE 5-HT1C RECEPTOR CONTAINS 8 HYDROPHOBIC DOMAINS AND IS X-LINKED SO MOLECULAR BRAIN RESEARCH LA English DT Article DE G-PROTEIN-COUPLED RECEPTOR; X-CHROMOSOME; XENOPUS OOCYTE ID BETA-ADRENERGIC-RECEPTOR; INJECTED XENOPUS OOCYTES; MESSENGER-RNA; RAT-BRAIN; FUNCTIONAL EXPRESSION; SEROTONIN RECEPTOR; CHOROID-PLEXUS; LIGAND-BINDING; PROTEIN; RHODOPSIN AB The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) exerts diverse physiological effects in the central and peripheral nervous systems and in smooth muscle by interacting with pharmacologically distinct membrane receptors. We report here the cDNA cloning of the mouse 5-HT1c receptor and its functional expression in Xenopus oocytes. This receptor possesses the unusual feature of containing eight hydrophobic domains capable of forming membrane-spanning a-helices, contrary to the usual '7-helix' paradigm for other membrane receptors that function through coupling to GTP-binding proteins. By hybridization analysis of Chinese hamster x mouse somatic cell hybrid fines, the gene for the receptor, designated Htr1c, has been assigned to the mouse X chromosome. C1 UNIV BRITISH COLUMBIA,BIOTECHNOL LAB,VANCOUVER V6T 1W5,BC,CANADA. UNIV BRITISH COLUMBIA,DEPT NEUROSCI,VANCOUVER V6T 1W5,BC,CANADA. UNIV BRITISH COLUMBIA,DEPT ZOOL,VANCOUVER V6T 1W5,BC,CANADA. CALTECH,DIV BIOL,PASADENA,CA 91125. CALTECH,DIV CHEM & CHEM ENGN,PASADENA,CA 91125. INDIANA UNIV,SCH MED,WALTHER ONCOL CTR,INDIANAPOLIS,IN 46202. NIID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. SANDOZ LTD,PRECLIN RES,CH-4002 BASEL,SWITZERLAND. NIMH,CELL BIOL LAB,BETHESDA,MD 20892. RP YU, L (reprint author), INDIANA UNIV,SCH MED,DEPT MED & MOLEC GENET,975 W WALNUT ST,INDIANAPOLIS,IN 46202, USA. FU NINDS NIH HHS [NS28190] NR 48 TC 63 Z9 65 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD SEP PY 1991 VL 11 IS 2 BP 143 EP 149 DI 10.1016/0169-328X(91)90116-F PG 7 WC Neurosciences SC Neurosciences & Neurology GA GF883 UT WOS:A1991GF88300006 PM 1661811 ER PT J AU DRISCOLL, WJ LEE, YC STROTT, CA AF DRISCOLL, WJ LEE, YC STROTT, CA TI ADRENOCORTICAL PREGNENOLONE-BINDING PROTEIN-ACTIVITY REQUIRES A SMALL HEAT-STABLE FACTOR - EVIDENCE THAT REGULATION BY PHOSPHORYLATION DEPHOSPHORYLATION OCCURS AT THE LEVEL OF THE FACTOR, NOT THE PROTEIN SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID PIG ADRENAL-CORTEX; THYMOCYTE GLUCOCORTICOID RECEPTORS; AVIAN PROGESTERONE-RECEPTOR; ALKALINE-PHOSPHATASE; STABILIZING FACTOR; SOLUBLE FRACTION; HORMONE BINDING; ACTIVATION; INACTIVATION; PURIFICATION AB The steroid-binding capacity of the adrenocortical pregnenolone-binding protein (PBP) is effectively destroyed by extreme temperature (boiling water for 2-5 min); however, the boiled preparation contains a factor that potentiates ligand binding when readed to native PBP. Treatment of the boiled fraction with calf intestinal alkaline phosphatase at pH 9 reverses the stimulatory effect on PBP activity. Additionally, if native PBP is first incubated with alkaline phosphatase, which converts it to a nonbinding form, activity can be fully restored in a dose-dependent manner by the addition of the boiled preparation. The factor (itself devoid of binding capacity) can also be generated by exposing native PBP to acidic conditions (pH 4). The molecule is small (mol wt, < 2000), as judged by Sephadex G-25 gel filtration and equilibrium dialysis. It is not retained on Concanavalin-A-Sepharose and is not extractable with a variety of organic solvents. The factor remains active after lyophilization and has a net negative charge at pH 7.4 (determined by DEAE-cellulose chromatography). While the binding capacity of native PBP is destroyed by a variety of proteases, the heat-stable factor is unaffected by similar treatment. Additionally, factor activity is not susceptible to RNase, DNase, or lipase digestion. Thus, the protein moiety of the PBP has an absolute requirement for a distinct phosphorylated heat-stable factor for expression of ligand-binding activity, and it may be through this factor that binding activity is regulated. It is not yet known whether the factor is acting allosterically or actually functions as part of the steroid-binding site. C1 NICHHD,ENDOCRINOL & REPROD RES BRANCH,ADRENAL CELL BIOL SECT,BLDG 10,ROOM B1-L400,BETHESDA,MD 20892. NR 34 TC 4 Z9 4 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD SEP PY 1991 VL 5 IS 9 BP 1229 EP 1238 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA GG940 UT WOS:A1991GG94000003 PM 1770949 ER PT J AU WOODGATE, R ENNIS, DG AF WOODGATE, R ENNIS, DG TI LEVELS OF CHROMOSOMALLY ENCODED UMU PROTEINS AND REQUIREMENTS FOR INVIVO UMUD CLEAVAGE SO MOLECULAR & GENERAL GENETICS LA English DT Article DE UMUD, UMUD', UMUC; CHEMILUMINESCENT IMMUNO-DETECTION; SOS MUTAGENESIS ID ESCHERICHIA-COLI K-12; SOS REGULATORY SYSTEM; RECA PROTEIN; INDUCED MUTAGENESIS; ULTRAVIOLET-LIGHT; UV-MUTAGENESIS; DNA-REPAIR; INDUCIBLE MUTAGENESIS; REPRESSOR CLEAVAGE; LEXA REPRESSOR AB Most of the inducible mutagenesis observed in Escherichia coli after treatment with many DNA damaging agents is dependent upon the products of the umuD,C operon. RecA-mediated proteolytic processing of UmuD yields a carboxyl-terminal fragment (UmuD') that is active for mutagenesis. Processing of UmuD is therefore a critical step in the fixation of mutations. In this paper we have analyzed the requirements for UmuD processing in vivo. Standard immuno-detection assays, couple with a sensitive chemiluminescence detection assay, have been utilized to probe levels of chromosomally encoded Umu proteins from whole-cell E. coli extracts. We found that the derepression of additional SOS gene products, other than RecA, was not required for UmuD processing. Moreover, efficient cleavage of UmuD was observed only in the presence of elevated levels of activated RecA, suggesting that efficient processing would occur only under conditions of severe DNA damage. Detection of chromosomally encoded Umu proteins has allowed us, for the first time, to measure directly the cellular steady-state levels of these proteins under various SOS inducing conditions. UmuD was present at approximately 180 copies per uninduced cell and was measured at approximately 2400 copies per cell in strains that lacked a functional repressor. Induced levels of UmuC were approximately 12-fold lower than UmuD with approximately 200 molecules per cell. These levels of cellular UmuC protein suggest that it functions through specific protein-DNA or protein-protein interactions, possibly as a lesion recognition protein or by interacting with DNA polymerase III. C1 NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. RP WOODGATE, R (reprint author), NICHHD,VIRUSES & CELLULAR BIOL SECT,BETHESDA,MD 20892, USA. NR 48 TC 125 Z9 125 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0026-8925 J9 MOL GEN GENET JI Mol. Gen. Genet. PD SEP PY 1991 VL 229 IS 1 BP 10 EP 16 PG 7 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA GF176 UT WOS:A1991GF17600002 PM 1654503 ER PT J AU WOODGATE, R LEVINE, AS KOCH, WH CEBULA, TA EISENSTADT, E AF WOODGATE, R LEVINE, AS KOCH, WH CEBULA, TA EISENSTADT, E TI INDUCTION AND CLEAVAGE OF SALMONELLA-TYPHIMURIUM UMUD PROTEIN SO MOLECULAR & GENERAL GENETICS LA English DT Article DE ESCHERICHIA-COLI UMUD, UMUD'; IMMUNODETECTION; CHEMILUMINESCENCE; SOS MUTAGENESIS ID ESCHERICHIA-COLI; DNA-REPAIR; UV-MUTAGENESIS; ULTRAVIOLET-LIGHT; SOS MUTAGENESIS; OPERON; RECA; LT2; MUTANTS; GENE AB SOS mutagenesis in prokaryotes is dependent upon the inducible activity of the chromosomally encoded UmuDC proteins, or homologous proteins such as MucAB or ImpCAB which are found on naturally occurring plasmids. Relative to Escherichia coli, however, Salmonella typhimurium is much less responsive to the mutagenic effects of DNA-damaging agents, despite the fact that it possesses both chromosomally and plasmid encoded umu-like operons. In E. coli, activation of the UmuD mutagenesis protein to UmuD' via RecA-mediated proteolysis is a critical step in the mutation fixation pathway. We have used a polyclonal antiserum raised against the E. coli UmuD and UmuD' proteins to show that S. typhimurium expresses cross-reacting material only after treatment with the DNA-damaging agent mitomycin C. The S. typhimurium umuDC operon, therefore, appears to be regulated by mechanisms similar to the E. coli umuDC operon. After induction, the S. typhimurium UmuD protein was processed to UmuD' in both S. typhimurium and E. coli. However, the S. typhimurium UmuD protein appears to be cleaved more efficiently than the E. coli UmuD protein under similar conditions. The data suggest that conversion of UmuD to the mutagenically active UmuD' is not the rate-limiting factor accounting for the weakly mutable phenotype of S. typhimurium. C1 US FDA,MOLEC BIOL BRANCH,WASHINGTON,DC 20204. OFF NAVAL RES,DIV BIOL SCI,ARLINGTON,VA 22217. RP WOODGATE, R (reprint author), NICHHD,VIRUSES & CELLULAR BIOL SECT,BLDG 6,ROOM 1A13,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 34 TC 19 Z9 20 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0026-8925 J9 MOL GEN GENET JI Mol. Gen. Genet. PD SEP PY 1991 VL 229 IS 1 BP 81 EP 85 PG 5 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA GF176 UT WOS:A1991GF17600011 PM 1910151 ER PT J AU FURUYA, H MEYER, UA GELBOIN, HV GONZALEZ, FJ AF FURUYA, H MEYER, UA GELBOIN, HV GONZALEZ, FJ TI POLYMERASE CHAIN REACTION-DIRECTED IDENTIFICATION, CLONING, AND QUANTIFICATION OF HUMAN CYP2C18 MESSENGER-RNA SO MOLECULAR PHARMACOLOGY LA English DT Article ID HUMAN-LIVER; NIFEDIPINE OXIDASE; RAT-LIVER; CDNA; EXPRESSION; PROTEINS; SEQUENCE; OXIDATION; ENZYME AB Sequencing of genomic polymerase chain reaction (PCR) products synthesized using primers generated from the CYP2C8 and CYP2C9 cDNAs revealed the presence of a new CYP2C gene in the human genome. Primers specific to exons of this new gene were used to perform PCR on human liver cDNA libraries and cDNA synthesized from human liver mRNA to generate a cDNA containing a complete cytochrome P450 amino acid reading frame. This cytochrome P450 cDNA, designated CYP2C18, displayed 85% and 87% nucleotide and 77% and 81% amino acid sequence similarities, respectively, with cDNAs and proteins corresponding to CYP2C8 and CYP2C9. cDNA-directed synthesis of CYP2C18 revealed a protein with relative M(r) 49,000 on sodium dodecyl sulfate-polyacrylamide gels, which is considerably less than that calculated from the deduced amino acid composition, M(r) 55,747. A preferred substrate for this enzyme has not been uncovered. Levels of CYP2C8, CYP2C9, and CYP2C18 mRNAs were examined in 17 human liver specimens using a PCR-based assay. CYP2C18 mRNA was found in all livers examined, albeit at mean levels 7-8-fold lower than those of mRNAs encoding CYP2C8 and CYP2C9. Marked interindividual differences in levels of expression of all three CYP2C mRNAs were also found. C1 NCI,MOLEC CARCINOGENESIS LAB,BLDG 37,ROOM 3E 24,BETHESDA,MD 20892. UNIV BASEL,BIOCTR,CH-4056 BASEL,SWITZERLAND. NR 22 TC 60 Z9 60 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD SEP PY 1991 VL 40 IS 3 BP 375 EP 382 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GF512 UT WOS:A1991GF51200008 PM 1896026 ER PT J AU BALLA, T BAUKAL, AJ ENG, S CATT, KJ AF BALLA, T BAUKAL, AJ ENG, S CATT, KJ TI ANGIOTENSIN-II RECEPTOR SUBTYPES AND BIOLOGICAL RESPONSES IN THE ADRENAL-CORTEX AND MEDULLA SO MOLECULAR PHARMACOLOGY LA English DT Article ID GLOMERULOSA CELLS; ADENYLATE-CYCLASE; BINDING; CALCIUM; DISCRIMINATION; NUCLEOTIDES; HEPATOCYTES; STIMULATION; ACTIVATION; INHIBITION AB Angiotensin II (AII) receptor subtypes and their potential coupling mechanisms were studied using recently developed peptide and nonpeptide antagonists in rat and bovine adrenal zona glomerulosa cells, as well as in membranes prepared from rat and bovine adrenal cortex and medulla. Comparison of the potencies of these novel antagonists to displace I-125-[Sar1,Ile8]AII from its binding sites revealed two distinct AII binding sites in membranes prepared from rat adrenal capsules (zona glomerulosa) and from rat adrenal inner zones containing the medulla. About 85% of the binding sites of the glomerulosa zone and 30% of those of the inner zones were of the AT1 subtype, with relative affinities for the nonpeptide antagonists Dup 753 and PD 123177 and the peptide antagonist CGP 42112A in the order of Dup 753 >> CGP 42112A > PD 123177. In contrast, the relative binding potencies for the other (AT2) population of binding sites were CGP 42112A > PD 123177 >> Dup 753. Neither AII nor its peptide antagonist [Sar1,Ile8]AII could distinguish between the two sets of binding sites. The effects of the new antagonists on functional responses of rat adrenal glomerulosa cells demonstrated that both AII-stimulated aldosterone production and the AII-induced inhibition of adrenocorticotropic hormone-stimulated cAMP formation were mediated by the AT1 receptor subtype. In bovine adrenals, only AT1 receptors were detected in membranes prepared from the cortex and the medulla, as well as in cultured glomerulosa cells. The relative inhibitory potency of Dup 753 was lower by an order of magnitude at bovine than at rat AT1 receptors. The inhibition of AII-induced aldosterone production by the various antagonists was closely correlated with their inhibitory potencies on I-125-[Sar1,Ile8]AII binding to bovine glomerulosa cells. These data suggest that the known effects of AII in adrenal glomerulosa cells are mediated through the AT1 receptor subtype and that the distribution and/or specificity of the AT2 receptors shows marked species variations. C1 NICHHD,ENDOCRINOL & REPROD RES BRANCH,BLDG 10,ROOM B1 L-400,BETHESDA,MD 20892. OI Balla, Tamas/0000-0002-9077-3335 NR 31 TC 150 Z9 151 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD SEP PY 1991 VL 40 IS 3 BP 401 EP 406 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GF512 UT WOS:A1991GF51200011 PM 1654513 ER PT J AU LLOYD, RV DULING, DR RUMYANTSEVA, GV MASON, RP BRIDSON, PK AF LLOYD, RV DULING, DR RUMYANTSEVA, GV MASON, RP BRIDSON, PK TI MICROSOMAL REDUCTION OF 3-AMINO-1,2,4-BENZOTRIAZINE 1,4-DIOXIDE TO A FREE-RADICAL SO MOLECULAR PHARMACOLOGY LA English DT Article ID ELECTRON-SPIN-RESONANCE; SR-4233; SUPEROXIDE; GENERATION; TOXIN; TOXICITY; AGENT; CELLS AB The drug SR 4233 (3-amino-1,2,4-benzotriazine 1,4-dioxide) is under pharmacological study as the lead compound in a new series of hypoxia-activated drugs, the benzotriazine N-oxides. However, the stable two- and four-electron-reduced metabolites of SR 4233, formed by the successive loss of the two oxygen atoms, are not pharmacologically active. In order to evaluate the possibility of an initial one-electron intermediate as the active species, we have used microsomal reduction and EPR spectroscopy to identify the first free radical reduction product. The unpaired electron is primarily centered on the 1-nitrogen, and the radical is best described as a nitroxide. Results with spin-trapping experiments show that reduction of SR 4233 to a free radical is followed by its air oxidation, resulting in the formation of the superoxide radical. Experiments with specific inhibitors suggest that the drug is being reduced by microsomal NADPH-cytochrome P-450 reductase. C1 NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709. MEMPHIS STATE UNIV,DEPT CHEM,MEMPHIS,TN 38152. NR 31 TC 96 Z9 96 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD SEP PY 1991 VL 40 IS 3 BP 440 EP 445 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GF512 UT WOS:A1991GF51200017 PM 1654517 ER PT J AU POST, RM FINDLING, RL KAHN, RS AF POST, RM FINDLING, RL KAHN, RS TI INTERFACES BETWEEN SEIZURES AND AFFECTIVE-DISORDERS - THE USES OF VISUALLY MAPPING THE EVOLUTION AND LONGITUDINAL COURSE OF AN ILLNESS SO MOUNT SINAI JOURNAL OF MEDICINE LA English DT Article RP POST, RM (reprint author), NIMH,BIOL PSYCHIAT BRANCH,BLDG 10,ROOM 3N212,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOUNT SINAI HOSPITAL PI NEW YORK PA BOX 1094 ONE GUSTAVE L LEVY PLACE ATTN: CIRCULATION ASST, NEW YORK, NY 10029-6574 SN 0027-2507 J9 MT SINAI J MED JI Mt. Sinai J. Med. PD SEP PY 1991 VL 58 IS 4 BP 310 EP 323 PG 14 WC Medicine, General & Internal SC General & Internal Medicine GA GH072 UT WOS:A1991GH07200008 PM 1944318 ER PT J AU MCGREGOR, DB EDWARDS, I WOLF, CR FORRESTER, LM CASPARY, WJ AF MCGREGOR, DB EDWARDS, I WOLF, CR FORRESTER, LM CASPARY, WJ TI ENDOGENOUS XENOBIOTIC ENZYME LEVELS IN MAMMALIAN-CELLS SO MUTATION RESEARCH LA English DT Article DE ENZYME LEVELS, ENDOGENOUS XENOBIOTIC; XENOBIOTIC METABOLISM, ENZYMES INVOLVED IN; MAMMALIAN CELLS, RESPONSE TO CHEMICALS; GLUTATHIONE-S-TRANSFERASE ID SPECIES-SPECIFIC DIFFERENCES; CHINESE-HAMSTER CELLS; V79 CELLS; MEDIATED MUTAGENESIS; HYDROGEN-PEROXIDE; METABOLISM; TOXICITY; DIMETHYLNITROSAMINE; BENZO(A)PYRENE; MUTAGENICITY AB The response of mammalian cell lines to chemicals depends, in part, on the exogenous activation system used for the induction of a biological response. This could be attributed to differences in the expression of enzymes involved in xenobiotic metabolism. We have measured the activities of benzo[a]pyrene hydroxylase, dimethylaminoazobenzene N-demethylase, catalase, superoxide dismutase, peroxidase and glutathione-S-transferase in human lymphoblast TK6, mouse lymphoma L5178Y, Chinese hamster ovary (CHO) and lung (V79) and mouse C3H10T1/2 cell lines as well as in primary hepatocytes and S9 preparations of liver from male F344 rats. Nitroreductase was also measured in some of these preparations. Human lymphoblast TK6 and mouse C3H10T1/2 cells had the capacity to metabolize dimethylaminoazobenzene and the latter cell line also metabolized benzo[a]pyrene, indicating the presence of constitutive mono-oxygenase activity. Cytochrome P450 could not be detected spectrophotometrically in the cell lines. Western blot analysis indicated that P450 from the P450IIA family is expressed in C3H10T1/2 cells. Reactivity was also observed with an antibody to P450IA2; however, the identity of this protein remains uncertain. Superoxide dismutase, catalase and peroxidase, which protect cells against oxygen radical damage, were found in all the cell lines and in rat hepatocytes and S9. The human lymphoblast TK6 cell line, however, had the least of each of these three enzymes. Glutathione-S-transferase activity was detected at varying levels in all cell types. Nitroreductase activity was high in S9 and Chinese hamster ovary cells and lower in mouse lymphoma and Chinese hamster V79 cells. C1 NIEHS,CANC GENET & MOLEC PATHOL SECT,POB 12233,RES TRIANGLE PK,NC 27709. INVERESK RES INT LTD,MUSSELBURGH EH21 7UB,SCOTLAND. IMPERIAL CANC RES FUND,EDINBURGH EH8 9XD,SCOTLAND. NR 50 TC 45 Z9 45 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD SEP PY 1991 VL 261 IS 1 BP 29 EP 39 DI 10.1016/0165-1218(91)90095-4 PG 11 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA GD316 UT WOS:A1991GD31600005 PM 1715512 ER PT J AU JONES, JC STEVNSNER, T MATTERN, MR BOHR, VA AF JONES, JC STEVNSNER, T MATTERN, MR BOHR, VA TI EFFECT OF SPECIFIC ENZYME-INHIBITORS ON REPLICATION, TOTAL GENOME DNA-REPAIR AND ON GENE-SPECIFIC DNA-REPAIR AFTER UV IRRADIATION IN CHO CELLS SO MUTATION RESEARCH LA English DT Article DE ENZYME INHIBITORS, EFFECT ON REPLICATION; REPLICATION, INHIBITION; TOTAL GENOME DNA REPAIR; INHIBITION OF DNA REPAIR; GENE-SPECIFIC DNA REPAIR; DIHYDROFOLATE REDUCTASE GENE; APHIDICOLIN; DNA POLYMERASE; RIBONUCLEOTIDE REDUCTASE; TOPOISOMERASE ID HAMSTER OVARY CELLS; TOPOISOMERASE-II; HUMAN-FIBROBLASTS; ACTIVE GENE; MAMMALIAN-CELLS; CLEAVAGE SITES; DHFR GENE; NOVOBIOCIN; CHROMATIN; DAMAGE AB We have studied the effect of some specific enzyme inhibitors on DNA repair and replication after UV damage in Chinese hamster ovary cells. The DNA repair was studied at the level of the average, overall genome and also in the active dihydrofolate reductase gene. Replication was measured in the overall genome. We tested inhibitors of DNA polymerase alpha and delta (aphidicolin), of poly(ADPr) polymerase (3-aminobenzamide), of ribonucleotide reductase (hydroxyurea), of topoisomerase I (camptothecin), and of topoisomerase II (merbarone, VP-16). In addition, we tested the effect of the potential topoisomerase I activator, beta-lapachone. All of these compounds inhibited genome replication and all topoisomerase inhibitors affected the overall genome repair; beta-lapachone stimulated it. None of these compounds had any effect on the gene-specific repair. C1 NCI,DIV CANC TREATMENT,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. SMITH KLINE BEECHAM PHARMACEUT,DEPT BIOMOLEC DISCOVERY,KING OF PRUSSIA,PA 19406. NR 36 TC 22 Z9 23 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD SEP PY 1991 VL 255 IS 2 BP 155 EP 162 DI 10.1016/0921-8777(91)90049-U PG 8 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA GF754 UT WOS:A1991GF75400004 PM 1656249 ER PT J AU KUNKEL, TA ROBERTS, JD SUGINO, A AF KUNKEL, TA ROBERTS, JD SUGINO, A TI THE FIDELITY OF DNA-SYNTHESIS BY THE CATALYTIC SUBUNIT OF YEAST DNA POLYMERASE-ALPHA ALONE AND WITH ACCESSORY PROTEINS SO MUTATION RESEARCH LA English DT Article DE FIDELITY; DNA POLYMERASE-ALPHA; DNA REPLICATION ID PRIMASE COMPLEX; DROSOPHILA-MELANOGASTER; INVITRO REPLICATION; SV40 ORIGIN; DELTA; CELLS; GAMMA AB The fidelity of DNA synthesis catalyzed by the 180-kDa catalytic subunit (p180) of DNA polymerase-alpha from Saccharomyces cerevisiae has been determined. Despite the presence of a 3' --> 5' exonuclease activity (Brooke et al., 1991, J. Biol. Chem., 266, 3005-3015), its accuracy is similar to several exonuclease-deficient DNA polymerases and much lower than other DNA polymerases that have associated exonucleolytic proofreading activity. Average error rates are 1/9900 and 1/12 000, respectively, for single base-substitution and minus-one nucleotide frameshift errors; the polymerase generates deletions as well. Similar error rates are observed with reactions containing the 180-kDa subunit plus an 86-kDa subunit (p86), or with these two polypeptides plus two additional subunits (p58 and p49) comprising the DNA primase activity required for DNA replication. Finally, addition of yeast replication factor-A (RF-A), a protein preparation that stimulates DNA synthesis and has single-stranded DNA-binding activity, yields a polymerization reaction with 7 polypeptides required for replication, yet fidelity remains low relative to error rates for semiconservative replication. The data suggest that neither exonucleolytic proofreading activity, the beta-subunit, the DNA primase subunits nor RF-A contributes substantially to base substitution or frameshift error discrimination by the DNA polymerase-alpha catalytic subunit. RP KUNKEL, TA (reprint author), NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709, USA. NR 25 TC 9 Z9 9 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD SEP-OCT PY 1991 VL 250 IS 1-2 BP 175 EP 182 DI 10.1016/0027-5107(91)90174-M PG 8 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA GL878 UT WOS:A1991GL87800018 PM 1944334 ER PT J AU BATES, H BRIDGES, BA WOODGATE, R AF BATES, H BRIDGES, BA WOODGATE, R TI MUTAGENIC DNA-REPAIR IN ESCHERICHIA-COLI .20. OVERPRODUCTION OF UMUD' PROTEIN RESULTS IN SUPPRESSION OF THE UMUC36 MUTATION IN EXCISION DEFECTIVE BACTERIA SO MUTATION RESEARCH LA English DT Article DE ERROR-PRONE REPAIR; UMUC36 MUTATION; UMUD PROTEIN; ULTRAVIOLET MUTAGENESIS; DNA REPAIR, MUTAGENIC ID RECA PROTEIN; SOS MUTAGENESIS; POLYMERASE-III; UV-MUTAGENESIS; CLEAVAGE; LIGHT; PURIFICATION; REPLICATION; REPRESSOR; STRAINS AB Overproduction of Umu+ or UmuD' protein by means of a gene carried on a multicopy plasmid suppressed the umuC36 phenotype and permitted induction of mutations by ultraviolet light. The umuC122::Tn5 phenotype was not suppressed. Suppression of the umuC36 phenotype was only seen when excision repair was blocked by acriflavine or by an uvrA or uvrB mutation. Cleavage of UmuD to UmuD' in SOS-induced cells was not dependent upon the presence of UmuC protein. The results are interpreted in terms of a revised model in which UmuC protein is envisaged as guiding UmuD' to RecA protein which has recognized and become bound to an appropriate DNA lesion. It is suggested that the umuC36 mutation gives rise to a protein with reduced affinity for UmuD' and that the effect of this can be compensated by an excess of UmuD'. C1 UNIV SUSSEX,MRC,CELL MUTAT UNIT,BRIGHTON BN1 9RR,ENGLAND. NICHHD,BETHESDA,MD 20892. NR 27 TC 12 Z9 12 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD SEP-OCT PY 1991 VL 250 IS 1-2 BP 199 EP 204 DI 10.1016/0027-5107(91)90176-O PG 6 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA GL878 UT WOS:A1991GL87800020 PM 1944336 ER PT J AU SHELBY, MD GUTIERREZESPELETA, GA GENEROSO, WM MCFEE, AF AF SHELBY, MD GUTIERREZESPELETA, GA GENEROSO, WM MCFEE, AF TI MOUSE DOMINANT LETHAL AND BONE-MARROW MICRONUCLEUS STUDIES ON METHYL VINYL SULFONE AND DIVINYL SULFONE SO MUTATION RESEARCH LA English DT Article DE MICRONUCLEUS STUDIES; METHYL VINYL SULFONE; DIVINYL SULFONE; DOMINANT LETHAL MUTATIONS; BONE MARROW ERYTHROCYTES; ACRYLAMIDE ID MALE-MICE; GERM-CELLS; ACRYLAMIDE; CARCINOGENICITY; MUTAGENICITY; SALMONELLA; ASSAYS; DNA AB Methyl vinyl sulfone and divinyl sulfone were tested for the induction of dominant lethal mutations and micronucleated bone-marrow erythrocytes in male mice. These chemicals were chosen for study because of their similarities in structure and chemical reactivity to acrylamide which is known to induce both effects. Following administration of the test compounds by intraperitoneal injection at the maximum tolerated doses, no evidence of induced dominant lethal mutations or micronucleated bone-marrow cells was observed for either chemical. It is concluded that structures and Michael reactivities similar to acrylamide are not sufficient to impart similar in vivo genetic toxicity to MVS and DVS. C1 OAK RIDGE ASSOCIATED UNIV,OAK RIDGE,TN 37831. OAK RIDGE NATL LAB,DIV BIOL,OAK RIDGE,TN 37831. RP SHELBY, MD (reprint author), NIEHS,POB 12233,RES TRIANGLE PK,NC 27709, USA. FU NIEHS NIH HHS [Y01-ES-20100, Y01-ES-20085] NR 17 TC 8 Z9 8 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD SEP-OCT PY 1991 VL 250 IS 1-2 BP 431 EP 437 DI 10.1016/0027-5107(91)90199-X PG 7 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA GL878 UT WOS:A1991GL87800043 PM 1944356 ER PT J AU GENEROSO, WM SHOURBAJI, AG PIEGORSCH, WW BISHOP, JB AF GENEROSO, WM SHOURBAJI, AG PIEGORSCH, WW BISHOP, JB TI DEVELOPMENTAL RESPONSE OF ZYGOTES EXPOSED TO SIMILAR MUTAGENS SO MUTATION RESEARCH LA English DT Article DE FETAL ANOMALIES; ZYGOTES; METHYL METHANESULFONATE; DIMETHYL SULFATE; DIETHYL SULFATE ID ETHYLENE-OXIDE; FETAL ANOMALIES; DEATH; MALFORMATIONS; MOUSE; MICE AB Exposure of mouse zygotes to ethylene oxide (EtO) or ethyl methanesulfonate (EMS) led to high incidences of fetal death and of certain classes of fetal malformations (Generoso et al., 1987, 1988; Rutledge and Generoso, 1989). These effects were not associated with induced chromosomal aberrations (Katoh et al., 1989) nor are they likely to be caused by gene mutations (Generoso et al., 1990). Nevertheless, the anomalies observed in these studies resemble the large class of stillbirths and sporadic defects in humans that are of unknown etiology, such as cleft palate, omphalocoel, clubfoot, hydrops and stillbirths (Czeizel, 1985; Oakley, 1986). Therefore, we continue to study the possible mechanisms relating to induction of these types of zygote-derived anomalies in mice. Effects of zygote exposure to the compounds methyl methanesulfonate (MMS), dimethyl sulfate (DMS), and diethyl sulfate (DES), which have similar DNA-binding properties as EtO and EMS, were studied. DMS and DES, but not MMS, induced effects that are similar to those induced by EtO and EMS. Thus, no site-specific alkylation product was identifiable as the critical target for these zygote-derived anomalies. We speculate that the developmental anomalies arose as a result of altered programming of gene expression during embryogenesis. C1 NIEHS,RES TRIANGLE PK,NC 27709. RP GENEROSO, WM (reprint author), OAK RIDGE NATL LAB,DIV BIOL,POB 2009,OAK RIDGE,TN 37738, USA. OI Piegorsch, Walter/0000-0003-2725-5604 FU NIEHS NIH HHS [Y01-ES-20085] NR 22 TC 28 Z9 29 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD SEP-OCT PY 1991 VL 250 IS 1-2 BP 439 EP 446 DI 10.1016/0027-5107(91)90200-8 PG 8 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA GL878 UT WOS:A1991GL87800044 PM 1944357 ER PT J AU KAUFMAN, EE NELSON, T AF KAUFMAN, EE NELSON, T TI AN OVERVIEW OF GAMMA-HYDROXYBUTYRATE CATABOLISM - THE ROLE OF THE CYTOSOLIC NADP+-DEPENDENT OXIDOREDUCTASE EC1.1.1.19 AND OF A MITOCHONDRIAL HYDROXYACID-OXOACID TRANSHYDROGENASE IN THE INITIAL, RATE-LIMITING STEP IN THIS PATHWAY SO NEUROCHEMICAL RESEARCH LA English DT Article ID SUCCINIC SEMI-ALDEHYDE; MAMMALIAN BRAIN; REDUCTASE; ACID; PURIFICATION; METABOLISM; BUTYROLACTONE; AMINOBUTYRATE; DEGRADATION; CONVERSION RP KAUFMAN, EE (reprint author), NIMH,CEREBRAL METAB LAB,BLDG 36,ROOM 1A-05,BETHESDA,MD 20892, USA. NR 41 TC 40 Z9 41 U1 0 U2 3 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0364-3190 J9 NEUROCHEM RES JI Neurochem. Res. PD SEP PY 1991 VL 16 IS 9 BP 965 EP 974 DI 10.1007/BF00965839 PG 10 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA GJ140 UT WOS:A1991GJ14000005 PM 1784339 ER PT J AU KAUFMAN, S AF KAUFMAN, S TI SOME METABOLIC RELATIONSHIPS BETWEEN BIOPTERIN AND FOLATE - IMPLICATIONS FOR THE METHYL TRAP HYPOTHESIS SO NEUROCHEMICAL RESEARCH LA English DT Article DE TETRAHYDROBIOPTERIN; METHYL TRAP HYPOTHESIS; METHYL TETRAHYDROFOLATE; METHYLENE TETRAHYDROFOLATE REDUCTASE; VITAMIN-B12 ID DIHYDROPTERIDINE REDUCTASE DEFICIENCY; DIHYDROFOLATE-REDUCTASE; PARTIAL PURIFICATION; HYPERPHENYLALANINEMIA; TETRAHYDROBIOPTERIN; PHENYLKETONURIA; HYDROXYLASE; BIOSYNTHESIS; DEFECTS; THERAPY AB Tetrahydrobiopterin and the folate coenzymes can reciprocally interact in ways that would be useful to the metabolic pathways subserved by both of these coenzymes. Thus, through one of the reactions catalyzed by methylene tetrahydrofolate reductase, 5-CH3-H-4-folate can regenerate BH4 from q-BH2 and qBH4 can provide an escape from the "methyl trap." RP KAUFMAN, S (reprint author), NIMH,NEUROCHEM LAB,9000 ROCKVILLE PIKE,BLDG 36,ROOM 3030,BETHESDA,MD 20892, USA. NR 40 TC 67 Z9 69 U1 0 U2 1 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0364-3190 J9 NEUROCHEM RES JI Neurochem. Res. PD SEP PY 1991 VL 16 IS 9 BP 1031 EP 1036 DI 10.1007/BF00965847 PG 6 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA GJ140 UT WOS:A1991GJ14000013 PM 1784330 ER PT J AU SMITH, CB AF SMITH, CB TI THE MEASUREMENT OF REGIONAL RATES OF CEREBRAL PROTEIN-SYNTHESIS INVIVO SO NEUROCHEMICAL RESEARCH LA English DT Article ID LOCAL-RATES; AMINO-ACIDS; ADULT RATS; BRAIN; LIVER; PLASTICITY; TURNOVER; AXOTOMY; YOUNG RP SMITH, CB (reprint author), NIMH,CEREBRAL METAB LAB,BLDG 36,ROOM 1A-05,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 27 TC 24 Z9 24 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0364-3190 J9 NEUROCHEM RES JI Neurochem. Res. PD SEP PY 1991 VL 16 IS 9 BP 1037 EP 1045 DI 10.1007/BF00965848 PG 9 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA GJ140 UT WOS:A1991GJ14000014 PM 1784331 ER PT J AU KLEE, CB AF KLEE, CB TI CONCERTED REGULATION OF PROTEIN-PHOSPHORYLATION AND DEPHOSPHORYLATION BY CALMODULIN SO NEUROCHEMICAL RESEARCH LA English DT Article DE CALMODULIN; KINASE; CALCINEURIN; PHOSPHATASE ID KINASE-II; AUTOPHOSPHORYLATION SITES; CATALYTIC SUBUNIT; CA-2+ CALMODULIN; DOMAIN-STRUCTURE; BINDING DOMAIN; CALCINEURIN-A; RAT-BRAIN; EXPRESSION; ACTIVATION AB The multiple functions of calmodulin in brain bring to light an apparent paradox in the mechanism of action of this multifunctional regulatory protein: How can the simultaneous calmodulin stimulation of enzymes with opposing functions, such as cyclic nucleotide phosphodiesterases and adenylate cyclase, which are responsible for the degradation and synthesis of cAMP, respectively, be physiologically significant? The same question applies to the simultaneous activation of protein kinases (in particular calmodulin kinase II) and a protein phosphatase (calcineurin). One could propose that the protein kinase(s) and the phosphatase may be located in different cells or in different cellular compartments, and are therefore not antagonizing each other. The same result could be achieved if the specific substrates of these enzymes have different cellular localizations. This does not seem to be the case. In many areas of the brain the two enzymes and their substrates coexist in the same cell. For example, the hippocampus is rich in calmodulin kinase II, calcineruin and substrates for the two enzymes. A more general scheme is presented here, based on different mechanisms of the calmodulin regulation of the two classes of enzyme, which helps to solve this apparent inconsistency in the mechanism of action of calmodulin. RP NCI, BIOCHEM LAB, BETHESDA, MD 20892 USA. NR 47 TC 119 Z9 120 U1 0 U2 0 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-3190 EI 1573-6903 J9 NEUROCHEM RES JI Neurochem. Res. PD SEP PY 1991 VL 16 IS 9 BP 1059 EP 1065 DI 10.1007/BF00965851 PG 7 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA GJ140 UT WOS:A1991GJ14000017 PM 1664495 ER PT J AU KETY, SS AF KETY, SS TI THE CIRCULATION, METABOLISM, AND FUNCTIONAL-ACTIVITY OF THE HUMAN BRAIN SO NEUROCHEMICAL RESEARCH LA English DT Article ID CEREBRAL GLUCOSE-UTILIZATION; BLOOD-FLOW; INTRAVENOUS (H2O)-O-15; SYSTEM RP KETY, SS (reprint author), NIH,ROOM 4C-116,BETHESDA,MD 20892, USA. NR 29 TC 2 Z9 2 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0364-3190 J9 NEUROCHEM RES JI Neurochem. Res. PD SEP PY 1991 VL 16 IS 9 BP 1073 EP 1078 DI 10.1007/BF00965853 PG 6 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA GJ140 UT WOS:A1991GJ14000019 PM 1784334 ER PT J AU WEBER, RFA CALOGERO, AE AF WEBER, RFA CALOGERO, AE TI PROLACTIN STIMULATES RAT HYPOTHALAMIC CORTICOTROPIN-RELEASING HORMONE AND PITUITARY ADRENOCORTICOTROPIN SECRETION INVITRO SO NEUROENDOCRINOLOGY LA English DT Article DE ADRENOCORTICOTROPIC HORMONE; CORTICOTROPIN-RELEASING HORMONE; HYPOTHALAMIC EXPLANTS; PITUITARY CELLS; PROLACTIN ID GONADOTROPIN-SECRETION; ADULT MALE; REPRODUCTIVE FUNCTIONS; FEMALE RATS; HYPERPROLACTINEMIA; INHIBITION; TESTOSTERONE; CORTICOSTERONE; INVOLVEMENT; INVIVO AB In rats hyperprolactinemia increases corticotropin-releasing hormone (CRH) concentration and secretion in hypophysial portal blood and the serum concentration of adrenocorticotropic hormone (ACTH). To determine whether the stimulatory effect of prolactin (PRL) on CRH and ACTH in vivo is exerted directly on the hypothalamus, hypothalamic explants and primary anterior pituitary cell cultures from adult male and female rats were used. Hypothalami explanted from male and female rats were preincubated during 90 min and treated for 30 min with rat PRL (rPRL) at concentrations of 10(-8), 10(-7), and 10(-6) M (about 200, 2,000, and 20,000 ng/ml, respectively), corticosterone at concentrations of 10(-7), 10(-6), and 10(-5) M (about 35, 350 and 3,500 ng/ml, respectively), ACTH at concentrations ranging from 10(-10) to 10(-7) M (0.46, 4.6, 46, and 460 ng/ml, respectively), and graded concentrations of testosterone or estradiol. Concentrations of immunoreactive CRH (iCRH) were measured by radioimmunoassay. rPRL at 10(-6) M stimulated iCRH secretion by 360 and 400% of the basal iCRH output (about 14 pg/hypothalamus), respectively, from hypothalami explanted from male and female rats. ACTH and corticosterone did not suppress rPRL (10(-6) M) induced iCRH secretion. Corticosterone at the concentration of 10(-6) M potentiated rPRL (10(-6) M) induced iCRH secretion in hypothalami explanted from male, but not female rats. Gonadal steroids had no effect either on the basal or rPRL (10(-6) M) stimulated iCRH secretion, with the exception of estradiol which augmented the response to 10(-6) M rPRL by about fivefold, but only at the concentration of 10(-8) M (about 2.7 ng/ml). Pituitary cells were treated with rPRL, corticosterone, gonadal steroids, or CRH for 4 h. The concentrations of ACTH in the culture medium were measured by radioimmunoassay. rPRL at the concentration of 10(-7) M caused an ACTH release of 169 +/- 8 and 185 +/- 14% of basal values (about 2,000 pg/10(5) cells), respectively, in pituitary cells explanted from male and female rats, whereas at the concentration of 10(-6) M rPRL caused a release of 265 +/- 15 (male) and 336 +/- 62% (female). Corticosterone suppressed PRL (10(-6) M) induced ACTH release in a dose-dependent fashion, but the ACTH secretion did not reach basal levels. Inhibition of CRH-induced ACTH release by corticosterone was not reversed by the addition of 10(-6) M rPRL. Gonadal steroids had no effect. Rat growth hormone did not influence iCRH or ACTH secretion in these systems. We conclude that the activation of the hypothalamic-pituitary-adrenal axis observed during the course of hyperprolactinemia may be explained by a direct stimulatory effect of PRL on both hypothalamic CRH and pituitary ACTH secretions. C1 NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. NR 25 TC 30 Z9 30 U1 0 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0028-3835 J9 NEUROENDOCRINOLOGY JI Neuroendocrinology PD SEP PY 1991 VL 54 IS 3 BP 248 EP 253 DI 10.1159/000125882 PG 6 WC Endocrinology & Metabolism; Neurosciences SC Endocrinology & Metabolism; Neurosciences & Neurology GA GB834 UT WOS:A1991GB83400009 PM 1658672 ER PT J AU GORELICK, PB ROMAN, GC AF GORELICK, PB ROMAN, GC TI VASCULAR DEMENTIA - IN SEARCH OF ANSWERS SO NEUROEPIDEMIOLOGY LA English DT Editorial Material C1 RUSH MED COLL,RUSH ALZHEIMER DIS CTR,CHICAGO,IL 60612. NIH,NEUROEPIDEMIOL BRANCH,BETHESDA,MD 20892. RP GORELICK, PB (reprint author), RUSH MED COLL,DEPT NEUROL SCI,CEREBROVASC DIS SECT,1725 W HARRISON,SUITE 1140,CHICAGO,IL 60612, USA. FU NIA NIH HHS [1K08 AG00350-03] NR 11 TC 4 Z9 4 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0251-5350 J9 NEUROEPIDEMIOLOGY JI Neuroepidemiology PD SEP-DEC PY 1991 VL 10 IS 5-6 BP 225 EP 227 DI 10.1159/000110275 PG 3 WC Public, Environmental & Occupational Health; Clinical Neurology SC Public, Environmental & Occupational Health; Neurosciences & Neurology GA GX243 UT WOS:A1991GX24300001 PM 1798422 ER PT J AU LYNCH, WP CZUB, S MCATEE, FJ HAYES, SF PORTIS, JL AF LYNCH, WP CZUB, S MCATEE, FJ HAYES, SF PORTIS, JL TI MURINE RETROVIRUS INDUCED SPONGIFORM ENCEPHALOPATHY - PRODUCTIVE INFECTION OF MICROGLIA AND CEREBELLAR NEURONS IN ACCELERATED CNS DISEASE SO NEURON LA English DT Article ID CENTRAL-NERVOUS-SYSTEM; LEUKEMIA-VIRUS-TB; C RNA VIRUS; IMMUNOHISTOCHEMICAL LOCALIZATION; PARALYTOGENIC MUTANT; SLOW DISEASE; MOUSE; MICE; PATHOGENESIS; CELLS AB We have examined the pathological lesions and sites of infection in mice inoculated with a highly neurovirulent recombinant wild mouse ecotropic retrovirus (FrCas(E)). The spongiform lesions appeared initially as swollen postsynaptic neuronal processes, progressing to swelling in neuronal cell bodies, all in the absence of detectable gliosis. Infection of neurons in regions of vacuolation was not detected. However, high level infection of cerebellar granule neurons was observed in the absence of cytopathology, wherein viral protein was found associated with both axons and dendrites. Infection of ramified and amoeboid microglial cells was associated with cytopathology in the brain stem, and endothelial cell-pericyte infection was found throughout the CNS. No evidence of defective retroviral expression was observed. These results are consistent with an indirect mechanism of retrovirus-induced neuropathology. C1 NIAID,ROCKY MTN LABS,VECTORS & PATHOGENS LAB,HAMILTON,MT 59840. RP LYNCH, WP (reprint author), NIAID,ROCKY MTN LABS,PERSISTENT VIRAL DIS LAB,HAMILTON,MT 59840, USA. NR 49 TC 81 Z9 81 U1 0 U2 0 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0896-6273 J9 NEURON JI Neuron PD SEP PY 1991 VL 7 IS 3 BP 365 EP 379 DI 10.1016/0896-6273(91)90289-C PG 15 WC Neurosciences SC Neurosciences & Neurology GA GG887 UT WOS:A1991GG88700002 PM 1654946 ER PT J AU PARRY, BL GERNER, RH WILKINS, JN HALARIS, AE CARLSON, HE HERSHMAN, JM LINNOILA, M MERRILL, J GOLD, PW GRACELY, R ALOI, J NEWTON, R AF PARRY, BL GERNER, RH WILKINS, JN HALARIS, AE CARLSON, HE HERSHMAN, JM LINNOILA, M MERRILL, J GOLD, PW GRACELY, R ALOI, J NEWTON, R TI CSF AND ENDOCRINE STUDIES OF PREMENSTRUAL-SYNDROME SO NEUROPSYCHOPHARMACOLOGY LA English DT Article DE PREMENSTRUAL SYNDROME; NEUROENDOCRINOLOGY; SEROTONIN; DOPAMINE; NOREPINEPHRINE; PROLACTIN; CORTISOL; THYROID ID TENSION SYNDROME; MHPG EXCRETION; PROLACTIN RESPONSES; DEPRESSION; RADIOIMMUNOASSAY; WOMEN; PROGESTERONE; CORTISOL; HORMONE; PLASMA AB Eight women with prospectively documented premenstrual syndrome (PMS) underwent multiple samplings for estradiol, progesterone, prolactin, cortisol, and plasma 3-methoxy-4-hydroxyphenylglycol (MHPG) during an asymptomatic midcycle (late follicular) and a symptomatic premenstrual (late luteal) phase of the menstrual cycle. Cerebrospinal fluid (CSF) was collected for analysis of MHPG, norepinephrine (NE), 5-hydroxyindoleacetic acid (5-HIAA), dihydroxyphenylacetic acid (DOPAC), gamma-aminobutyric acid (GABA), homovanillic acid (HVA), tyrosine, tryptophan, beta-endorphin, prostaglandins, adrenocorticotropic hormone (ACTH), and arginine vasopressin (AVP). In subsequent months, a dexamethasone suppression test (DST) and a thyrotropin-releasing hormone (TRH) stimulation test were performed during midcycle and premenstrual phases. Significant results included increased CSF concentrations of MHPG in the premenstrual, as compared with the midcycle, phase of the cycle, and increased plasma cortisol concentrations during the midcycle phase. The DST showed a 62% overall rate of nonsuppression, irrespective of menstrual cycle phase. Though there were no abnormalities of thyrotropin-stimulating hormone (TSH) after TRH stimulation, the mean delta maximum prolactin values after TRH stimulation were higher than reported normal values both at midcycle and premenstrually. These pilot data suggest hormonal axes that might be worthy of further systematic investigation in future studies of PMS. C1 NIMH,NIAAA,DICBR,CLIN STUDIES LAB,BETHESDA,MD 20892. UNIV CALIF LOS ANGELES,INST NEUROPSYCHIAT,LOS ANGELES,CA 90024. BRENTWOOD VET ADM MED CTR,BRENTWOOD,CA. WADSWORTH VET ADM HOSP,LOS ANGELES,CA. RP PARRY, BL (reprint author), UNIV CALIF SAN DIEGO,DEPT PSYCHIAT,T-004,LA JOLLA,CA 92093, USA. FU NCRR NIH HHS [RR-865, RR-05756]; NIMH NIH HHS [MH-28343] NR 41 TC 35 Z9 35 U1 1 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0893-133X J9 NEUROPSYCHOPHARMACOL JI Neuropsychopharmacology PD SEP PY 1991 VL 5 IS 2 BP 127 EP 137 PG 11 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GC115 UT WOS:A1991GC11500006 PM 1930615 ER PT J AU GARRUTO, RM AF GARRUTO, RM TI PACIFIC PARADIGMS OF ENVIRONMENTALLY-INDUCED NEUROLOGICAL DISORDERS - CLINICAL, EPIDEMIOLOGIC AND MOLECULAR PERSPECTIVES SO NEUROTOXICOLOGY LA English DT Article; Proceedings Paper CT 8TH INTERNATIONAL NEUROTOXICOLOGY CONF : ROLE OF TOXICANTS IN NEUROLOGICAL DISORDERS CY OCT 01-04, 1990 CL LITTLE ROCK, AR SP UNION CARBIDE, US EPA, HLTH EFFECTS RES LAB, US FDA, NATL CTR TOXICOL RES, NIEHS, ARKANSAS DEPT HLTH, CIBA GEIGY, DUPONT CO, HASKELL LAB TOXICOL & IND MED, EASTMAN KODAK, HLTH & ENVIRONM LABS, PROCTER & GAMBLE DE AMYOTROPHIC LATERAL SCLEROSIS; PARKINSONISM-DEMENTIA; ALZHEIMER DISEASE; ALUMINUM INTOXICATION; EXPERIMENTAL MODELS ID AMYOTROPHIC-LATERAL-SCLEROSIS; TANGLE-BEARING NEURONS; CENTRAL-NERVOUS-SYSTEM; ORALLY-ADMINISTERED ALUMINUM; 2ND METACARPAL BONE; PARKINSONISM-DEMENTIA; ALZHEIMERS-DISEASE; NEUROFIBRILLARY TANGLES; GASTROINTESTINAL ABSORPTION; CYTOSKELETAL PROTEINS AB During the past quarter century biomedical scientists have begun to recognize the unique opportunities for studying disease etiology and mechanisms of pathogenesis in non-Western anthropological populations with focal, endemic diseases. Such natural experiments as they are called, are important paradigms for solving etiological and epidemiological problems of widespread medical significance, with an ultimate goal towards treatment and prevention. The systematic search for etiological factors and mechanisms of pathogenesis of neurodegenerative disorders is perhaps nowhere better exemplified than in the western Pacific. During the past three decades, the opportunistic and multidisciplinary study of hyperendemic foci of amyotrophic lateral sclerosis and parkinsonism-dementia which occur in different cultures, in different ecological zones and among genetically divergent populations have served as natural models that have had a major impact on our thinking and enhanced our understanding of these and other neurodegenerative disorders such as Alzheimer disease and the process of early neuronal aging. Our cross-disciplinary approach to these intriguing neurobiological problems and the accumulated epidemiological, genetic, cellular and molecular evidence strongly implicates environmental factors in their causation, specifically the role of aluminum and its interaction with calcium in neuronal degeneration. As a direct consequence of our studies in these Pacific populations, we have undertaken the long-term development of experimental models of neuronal degeneration, in an attempt to understand the cellular and molecular mechanisms by which these toxicants affect the central nervous system. Our experimental studies have resulted in the establishment of an aluminum-induced chronic myelopathy in rabbits and the development of neurofilamentous lesions after low-dose aluminum administration in cell culture. These studies clearly demonstrate the philosophy that chronic rather than acute experimental models of toxicity are necessary in order to enhance our understanding of human neurodegenerative disorders with long-latency and slow progression. Finally, the ultimate significance of these Pacific paradigms may well depend on our ability to comprehensively evaluate and synthesize the growing body of relevant scientific data from other human disorders and from widely divergent academic fields, as well as our ability to recognize emerging new models in nature. RP GARRUTO, RM (reprint author), NIH,CENT NERVOUS SYST STUDIES LAB,BLDG 36,ROOM 5B21,BETHESDA,MD 20892, USA. NR 212 TC 78 Z9 78 U1 1 U2 2 PU INTOX PRESS INC PI LITTLE ROCK PA PO BOX 24865, LITTLE ROCK, AR 72221 SN 0161-813X J9 NEUROTOXICOLOGY JI Neurotoxicology PD FAL PY 1991 VL 12 IS 3 BP 347 EP 377 PG 31 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA GF180 UT WOS:A1991GF18000004 PM 1745428 ER PT J AU PASCUALLEONE, A DHUNA, A ANDERSON, DC AF PASCUALLEONE, A DHUNA, A ANDERSON, DC TI LONG-TERM NEUROLOGICAL COMPLICATIONS OF CHRONIC, HABITUAL COCAINE ABUSE SO NEUROTOXICOLOGY LA English DT Article; Proceedings Paper CT 8TH INTERNATIONAL NEUROTOXICOLOGY CONF : ROLE OF TOXICANTS IN NEUROLOGICAL DISORDERS CY OCT 01-04, 1990 CL LITTLE ROCK, AR SP UNION CARBIDE, US EPA, HLTH EFFECTS RES LAB, US FDA, NATL CTR TOXICOL RES, NIEHS, ARKANSAS DEPT HLTH, CIBA GEIGY, DUPONT CO, HASKELL LAB TOXICOL & IND MED, EASTMAN KODAK, HLTH & ENVIRONM LABS, PROCTER & GAMBLE DE COCAINE; DEPENDENCY; CHRONIC ABUSE; COMPUTED TOMOGRAPHY (CT); BRAIN; ELECTROENCEPHALOGRAPHY; EEG; LONG-TERM NEUROLOGICAL COMPLICATIONS; BRAIN ATROPHY; COGNITIVE DYSFUNCTION AB We review and expand the results of several previously published studies on computed tomographic and electroencephalographic correlates of chronic, habitual cocaine abuse in order to present a complete spectrum of the longterm neurological complications of cocaine abuse. We argue that prolonged and frequent cocaine abuse leads to biochemical and vascular cerebral insults that eventually may cause functional and structural abnormalities affecting predominantly the fronto-temporal areas. C1 UNIV MINNESOTA,DEPT NEUROL,MINNEAPOLIS,MN 55455. HENNEPIN CTY MED CTR,DEPT NEUROL,MINNEAPOLIS,MN 55415. RP PASCUALLEONE, A (reprint author), NINCDS,MED NEUROL BRANCH,BLDG 10,ROOM 5N226,BETHESDA,MD 20892, USA. RI Pascual-Leone, Alvaro/G-6566-2011 NR 23 TC 26 Z9 26 U1 1 U2 2 PU INTOX PRESS INC PI LITTLE ROCK PA PO BOX 24865, LITTLE ROCK, AR 72221 SN 0161-813X J9 NEUROTOXICOLOGY JI Neurotoxicology PD FAL PY 1991 VL 12 IS 3 BP 393 EP 400 PG 8 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA GF180 UT WOS:A1991GF18000007 PM 1745431 ER PT J AU STRONG, MJ GARRUTO, RM AF STRONG, MJ GARRUTO, RM TI POTENTIATION IN THE NEUROTOXIC INDUCTION OF EXPERIMENTAL CHRONIC NEURODEGENERATIVE DISORDERS - N-BUTYL BENZENESULFONAMIDE AND ALUMINUM-CHLORIDE SO NEUROTOXICOLOGY LA English DT Article; Proceedings Paper CT 8TH INTERNATIONAL NEUROTOXICOLOGY CONF : ROLE OF TOXICANTS IN NEUROLOGICAL DISORDERS CY OCT 01-04, 1990 CL LITTLE ROCK, AR SP UNION CARBIDE, US EPA, HLTH EFFECTS RES LAB, US FDA, NATL CTR TOXICOL RES, NIEHS, ARKANSAS DEPT HLTH, CIBA GEIGY, DUPONT CO, HASKELL LAB TOXICOL & IND MED, EASTMAN KODAK, HLTH & ENVIRONM LABS, PROCTER & GAMBLE DE CHRONIC MYELOPATHY; PLASTICIZER; SYNERGISM; TRACE METALS; NEUROTOXICITY ID CYTOSKELETAL PROTEIN ABNORMALITIES; MOTOR NEURON DISEASE; NEUROFIBRILLARY DEGENERATION; MAYTANSINE; HENS; IDPN AB Repeated monthly intracisternal inoculations of N-butyl benzenesulfonamide induced a chronic, slowly progressive myelopathy in young adult New Zealand white rabbits that was manifested by hyperreflexia, spasticity, hypertonia, gait impairment and altered tonic immobility responses. The neuropathological features consisted of scattered neuroaxonal spheroids, fusiform distention of the intramedullary portions of the spinal cord ventral roots and, as defined by microtubule-associated protein-2 (MAP 2) immunoreactivity, an initial distention and subsequent loss of dendritic processes in neurons of the nucleus motoris lateralis with the perikaryon of these cells remaining intact. A similar chronic progressive myelopathy was induced by repeated low dose intracisternal inoculations of aluminum chloride in New Zealand white rabbits. However, the neuropathological changes were more extensive and consisted of dendritic, axonal and perikaryal inclusions of phosphorylated and nonphosphorylated neurofilament localized to spinal motor neurons in the nucleus motoris medialis, substantia grisea intermedia and select brainstem nuclei with only minimal involvement of the nucleus motoris lateralis. The co-administration of these two neurotoxins over the course of 8 months induced striking behavioral changes as well as a fulminant myelopathy. This was accompanied by a loss of neuronal perikarya in the nucleus motoris lateralis and topographically extensive neocortical neurofilamentous degeneration. These features suggest that potentiation occurs when the two toxins are co-administered, a view supported by an estimation of the co-neurotoxicity coefficient (CNC > 1). Our results have implications for understanding human neurodegenerative disorders in which potentiation of insults may occur, producing a clinical and neuropathological disease state not expected from either agent alone. C1 NIH,CENT NERVOUS SYST STUDIES LAB,BETHESDA,MD 20892. RP STRONG, MJ (reprint author), UNIV WESTERN ONTARIO HOSP,DEPT CLIN NEUROL SCI,339 WINDERMERE RD,LONDON N6A 5A5,ONTARIO,CANADA. RI Strong, Michael/H-9689-2012 NR 48 TC 7 Z9 7 U1 0 U2 0 PU INTOX PRESS INC PI LITTLE ROCK PA PO BOX 24865, LITTLE ROCK, AR 72221 SN 0161-813X J9 NEUROTOXICOLOGY JI Neurotoxicology PD FAL PY 1991 VL 12 IS 3 BP 415 EP 425 PG 11 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA GF180 UT WOS:A1991GF18000009 PM 1745433 ER PT J AU YASUI, M YASE, Y OTA, K GARRUTO, RM AF YASUI, M YASE, Y OTA, K GARRUTO, RM TI EVALUATION OF MAGNESIUM, CALCIUM AND ALUMINUM METABOLISM IN RATS AND MONKEYS MAINTAINED ON CALCIUM-DEFICIENT DIETS SO NEUROTOXICOLOGY LA English DT Article; Proceedings Paper CT 8TH INTERNATIONAL NEUROTOXICOLOGY CONF : ROLE OF TOXICANTS IN NEUROLOGICAL DISORDERS CY OCT 01-04, 1990 CL LITTLE ROCK, AR SP UNION CARBIDE, US EPA, HLTH EFFECTS RES LAB, US FDA, NATL CTR TOXICOL RES, NIEHS, ARKANSAS DEPT HLTH, CIBA GEIGY, DUPONT CO, HASKELL LAB TOXICOL & IND MED, EASTMAN KODAK, HLTH & ENVIRONM LABS, PROCTER & GAMBLE DE CALCIUM; ALUMINUM; MAGNESIUM; CENTRAL NERVOUS SYSTEM; BONE; MINERAL-DEFICIENT DIETS ID AMYOTROPHIC LATERAL SCLEROSIS; BONE; DEGENERATION; PARKINSONISM; DEMENTIA AB The epidemiology of amyotrophic lateral sclerosis (ALS) in the Western Pacific indicates that low concentrations of calcium (Ca) and magnesium (Mg) and high levels of aluminum (Al) in soil and water in-these foci are etiologically important. To determine the biochemical derangements and metal deposition induced by chronic dietary deficiencies of Ca, we maintained experimental animals on several regimens. Male Wistar rats, weighing 100g, were fed either a standard diet, low Ca diet, low Ca-Mg diet, or low Ca-Mg diet with high Al for 90 days. Ca, Mg and Al content was determined in central nervous system (CNS) tissues and bone using inductively coupled plasma emission spectrometry (ICP). In separate studies, five male Japanese macaques (Macaca fuscata), weighing 3.5 to 5 kg, were fed alternately with diets, normal in Ca, low in Ca, low in Mg, low in Ca-Mg, or low in Ca-Mg with added Al for four-week periods. Serum Ca, Mg, Al, parathyroid hormone (PTH), bone Gla-protein (BGP) and alkaline phosphatase (ALP) were measured after feeding each dietary regimen. Ca and Mg levels in lumbar vertebrae and femur were significantly reduced and bone Al levels were significantly increased in rats fed diets deficient in Ca alone or diets low in Ca-Mg with or without added Al. Al content in bones was also higher in rats fed the Ca deficient diets. In monkeys fed the low Ca-Mg diet with added Al, reduced levels of serum Ca and Mg, serum PTH, BGP, and ALP were apparent. Our data support the conjecture that deranged bone mineralization induced by chronic dietary deficiency of Ca accelerates mobilization of Ca and Mg from bone and deposition in brain. C1 WAKAYAMA MED COLL,DEPT LAB MED,WAKAYAMA 640,JAPAN. NIH,BETHESDA,MD 20892. RP YASUI, M (reprint author), WAKAYAMA MED COLL,DIV NEUROL DIS,9 BANCHO,WAKAYAMA 640,JAPAN. NR 23 TC 19 Z9 19 U1 1 U2 1 PU INTOX PRESS INC PI LITTLE ROCK PA PO BOX 24865, LITTLE ROCK, AR 72221 SN 0161-813X J9 NEUROTOXICOLOGY JI Neurotoxicology PD FAL PY 1991 VL 12 IS 3 BP 603 EP 614 PG 12 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA GF180 UT WOS:A1991GF18000025 PM 1745443 ER PT J AU YASUI, M YASE, Y OTA, K GARRUTO, RM AF YASUI, M YASE, Y OTA, K GARRUTO, RM TI ALUMINUM DEPOSITION IN THE CENTRAL-NERVOUS-SYSTEM OF PATIENTS WITH AMYOTROPHIC-LATERAL-SCLEROSIS FROM THE KII PENINSULA OF JAPAN SO NEUROTOXICOLOGY LA English DT Article; Proceedings Paper CT 8TH INTERNATIONAL NEUROTOXICOLOGY CONF : ROLE OF TOXICANTS IN NEUROLOGICAL DISORDERS CY OCT 01-04, 1990 CL LITTLE ROCK, AR SP UNION CARBIDE, US EPA, HLTH EFFECTS RES LAB, US FDA, NATL CTR TOXICOL RES, NIEHS, ARKANSAS DEPT HLTH, CIBA GEIGY, DUPONT CO, HASKELL LAB TOXICOL & IND MED, EASTMAN KODAK, HLTH & ENVIRONM LABS, PROCTER & GAMBLE DE ALUMINUM; AMYOTROPHIC LATERAL SCLEROSIS; CALCIUM MAGNESIUM RATIO; CENTRAL NERVOUS SYSTEM ID CALCIUM; DEGENERATION; MAGNESIUM; BONE AB Chronic dietary deficiency of calcium (Ca) and magnesium (Mg) with excessive intake of aluminum (Al) and manganese (Mn) has been implicated in the pathogenesis of high incidence amyotrophic lateral sclerosis (ALS) in the Western Pacific. We report two cases of ALS from the Kii Peninsula of Japan with markedly elevated concentrations of Al in central nervous system (CNS) tissues. Six pathologically verified cases of ALS and five neurologically normal controls were studied. Levels of Al, Ca and phosphorus (P) were determined simultaneously by neutron activation analysis (NAA), and Mg concentration was measured by inductively coupled plasma emission spectrometry (ICP) in 26 CNS regions. Al concentrations in the precentral gyrus, internal capsule, crus cerebri and spinal cord were significantly increased in two ALS patients, compared with those of controls. Mean Al concentrations of the 26 CNS regions in these two patients were also higher than those of controls and of the four other ALS cases (p < 0.01). By contrast, Mg concentrations in the 26 CNS regions were markedly reduced in the ALS cases, compared with controls (p < 0.01), and the Ca/Mg ratios were significantly increased in the ALS cases (p < 0.01). Our data indicate that high-incidence ALS in the Western Pacific may result from Ca-Mg dysmetabolism with resultant deposition of Al. C1 WAKAYAMA MED COLL,DEPT LAB MED,WAKAYAMA 640,JAPAN. NIH,BETHESDA,MD 20892. RP YASUI, M (reprint author), WAKAYAMA MED COLL,DIV NEUROL DIS,9 BANCHO,WAKAYAMA 640,JAPAN. NR 18 TC 31 Z9 31 U1 0 U2 0 PU INTOX PRESS INC PI LITTLE ROCK PA PO BOX 24865, LITTLE ROCK, AR 72221 SN 0161-813X J9 NEUROTOXICOLOGY JI Neurotoxicology PD FAL PY 1991 VL 12 IS 3 BP 615 EP 620 PG 6 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA GF180 UT WOS:A1991GF18000026 PM 1745444 ER PT J AU HOLBROOK, NJ FORNACE, AJ AF HOLBROOK, NJ FORNACE, AJ TI RESPONSE TO ADVERSITY - MOLECULAR CONTROL OF GENE ACTIVATION FOLLOWING GENOTOXIC STRESS SO NEW BIOLOGIST LA English DT Review DE DNA DAMAGE; AP-1; IMMEDIATE EARLY GENES; TRANSCRIPTIONAL CONTROL; PROTEIN KINASE; PHORBOL ESTER; UV; X-RAY ID DNA-DAMAGING AGENTS; MESSENGER-RNA; IONIZING-RADIATION; UV-IRRADIATION; PHORBOL ESTER; HUMAN-CELLS; SACCHAROMYCES-CEREVISIAE; RIBONUCLEOTIDE REDUCTASE; ULTRAVIOLET-RADIATION; POSITIVE CONTROL C1 NCI,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. RP HOLBROOK, NJ (reprint author), NIA,GERONTOL RES CTR,MOLEC GENET LAB,BALTIMORE,MD 21224, USA. NR 59 TC 221 Z9 222 U1 0 U2 3 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD SEP PY 1991 VL 3 IS 9 BP 825 EP 833 PG 9 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK724 UT WOS:A1991GK72400002 PM 1931825 ER PT J AU HOEHE, MR CAENAZZO, L MARTINEZ, MM HSIEH, WT MODI, WS GERSHON, ES BONNER, TI AF HOEHE, MR CAENAZZO, L MARTINEZ, MM HSIEH, WT MODI, WS GERSHON, ES BONNER, TI TI GENETIC AND PHYSICAL MAPPING OF THE HUMAN CANNABINOID RECEPTOR GENE TO CHROMOSOME-6Q14-Q15 SO NEW BIOLOGIST LA English DT Article DE GENETIC LINKAGE MAPPING; INSITU HYBRIDIZATION; POLYMORPHIC MARKER ID ADENYLATE-CYCLASE; ALPHA-SUBUNIT; HUMAN GENOME; LINKAGE MAP; SHORT ARM; RAT-BRAIN; INHIBITION; HYBRIDIZATION; GONADOTROPIN; PHARMACOLOGY C1 NIMH,CELL BIOL LAB,BETHESDA,MD 20892. NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC DYNCORP,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21702. RP HOEHE, MR (reprint author), NIMH,CLIN NEUROGENET BRANCH,BETHESDA,MD 20892, USA. RI Martinez, Maria/B-3111-2013 OI Martinez, Maria/0000-0003-2180-4537 NR 37 TC 65 Z9 68 U1 0 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD SEP PY 1991 VL 3 IS 9 BP 880 EP 885 PG 6 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK724 UT WOS:A1991GK72400009 PM 1931832 ER PT J AU KALMAN, M MURPHY, H CASHEL, M AF KALMAN, M MURPHY, H CASHEL, M TI RHLB, A NEW ESCHERICHIA-COLI K-12 GENE WITH AN RNA HELICASE-LIKE PROTEIN-SEQUENCE MOTIF, ONE OF AT LEAST 5 SUCH POSSIBLE GENES IN A PROKARYOTE SO NEW BIOLOGIST LA English DT Article DE D-E-A-D BOX PROTEINS; DEGENERATE PRIMER PCR; ESCHERICHIA-COLI K-12; RHLB; RNA HELICASE-LIKE GENES ID EUKARYOTIC INITIATION-FACTORS; POLYMERASE CHAIN-REACTION; TRANSLATION INITIATION; MESSENGER-RNA; PUTATIVE HELICASES; IDENTIFICATION; YEAST; DNA; CHROMOSOME; MUTATION C1 NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. NR 38 TC 59 Z9 62 U1 0 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD SEP PY 1991 VL 3 IS 9 BP 886 EP 895 PG 10 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK724 UT WOS:A1991GK72400010 PM 1931833 ER PT J AU GOODWIN, FK GORDIS, E AF GOODWIN, FK GORDIS, E TI EXPRESSING MORAL OUTRAGE SO NEW BIOLOGIST LA English DT Letter C1 NIAAA,BETHESDA,MD 20892. NR 1 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD SEP PY 1991 VL 3 IS 9 BP 907 EP 908 PG 2 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK724 UT WOS:A1991GK72400012 PM 1931835 ER PT J AU BHATIA, K HUPPI, K MCKEITHAN, T SIWARSKI, D MUSHINSKI, JF MAGRATH, I AF BHATIA, K HUPPI, K MCKEITHAN, T SIWARSKI, D MUSHINSKI, JF MAGRATH, I TI MOUSE BCL-3 - CDNA STRUCTURE, MAPPING AND STAGE-DEPENDENT EXPRESSION IN LYMPHOCYTES-B SO ONCOGENE LA English DT Note ID LIGHT CHAIN GENE; CELL-CYCLE CONTROL; CHROMOSOME-TRANSLOCATION; LEUKEMIA; DIFFERENTIATION; BREAKPOINT; LYMPHOMAS; SEQUENCE; CLONING; ACTIVATION AB Human B-cell chronic lymphocytic leukemias (CLLs) are malignancies of mature B lymphocytes. A subset of these tumors is associated with a non-random t(14; 19) translocation (Ueshima et al., 1985). Recently a gene (bcl-3) has been identified in the region adjacent to the chromosome 19 breakpoint in this translocation (McKeithan et al., 1987; Ohno et al., 1990). We now report the isolation of cDNA clones of mouse bcl-3. The mouse bcl-3-coding region is 1746 bp long and exhibits 80% identity with human bcl-3 at both the nucleotide and amino acid level. The bcl-3 locus maps to the proximal end of mouse chromosome 7, which is syntenic to human chromosome 19. The bcl-3 probe readily detects particularly abundant amounts of a 1.8 kb mRNA in mouse tumors consisting of follicular center mature B cells and large pre-B cells, but not in small pre-B cells. The bcl-3 pattern of expression is distinctive in the spectrum of B-cell maturation in that bcl-3 transcripts are particularly abundant in B-cell lines immortalized just prior to Ig switch. The bcl-3 pattern of expression also bears close resemblance to that of bcl-2 (Gurfinkel et al., 1987), which is frequently associated with human B follicular lymphomas {t(14;18)} and some chronic lymphocytic leukemias (Adachi et al., 1989; 1990; Adachi & Tsujimoto, 1989). C1 UNIV CHICAGO,DEPT PATHOL,CHICAGO,IL 60637. UNIV CHICAGO,DEPT MED,CHICAGO,IL 60637. NCI,GENET LAB,BETHESDA,MD 20892. RP BHATIA, K (reprint author), NCI,PEDIAT BRANCH,LYMPHOMA BIOL SECT,BETHESDA,MD 20892, USA. FU NCI NIH HHS [CA49207] NR 20 TC 28 Z9 28 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0950-9232 J9 ONCOGENE JI Oncogene PD SEP PY 1991 VL 6 IS 9 BP 1569 EP 1573 PG 5 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA GX274 UT WOS:A1991GX27400011 PM 1923524 ER PT J AU WAKISAKA, S KAJANDER, KC BENNETT, GJ AF WAKISAKA, S KAJANDER, KC BENNETT, GJ TI ABNORMAL SKIN TEMPERATURE AND ABNORMAL SYMPATHETIC VASOMOTOR INNERVATION IN AN EXPERIMENTAL PAINFUL PERIPHERAL NEUROPATHY SO PAIN LA English DT Article DE CAUSALGIA; CUTANEOUS THERMOREGULATION; DYSAUTONOMIA; NEUROPATHIC PAIN; REFLEX SYMPATHETIC DYSTROPHY; SYMPATHETICALLY MAINTAINED PAIN; VASOMOTOR INNERVATION ID C-NOCICEPTORS; CAT HINDLIMB; MECHANISMS; CAUSALGIA; NEURONS; RAT; REFLEX; MODEL; MONONEUROPATHY; HYPERALGESIA AB A chronic constriction injury to the sciatic nerve of the rat produces a neuropathic pain syndrome that has many of the symptoms that are seen in humans with painful peripheral neuropathy. In particular, both the clinical and experimental conditions are accompanied by an abnormality of cutaneous temperature regulation in the painful area. A time course study was made of this phenomenon in the experimental model. In normal rats, there is little or no difference between the temperature of the two hind paws (plantar skin). After nerve injury, however, approximately 75% of the rats (N = 30) had abnormally large (greater than +/- 0.9-degrees-C) temperature differences (DELTA-T) between the affected and sham-operated sides. The abnormal DELTA-Ts could be either positive or negative, i.e., the affected side could be hotter or colder than normal. For individual cases, the temperature abnormality was highly variable over time periods of hours to days; abnormally hot skin could switch to being abnormally cold, and vice versa, and small DELTA-Ts in the normal range could switch between abnormal extremes. Despite this individual variability, the average DELTA-T of the group as a whole displayed a clear evolution over the course of the 30-day observation period: abnormally hot initially and progressing to abnormally cold. A parallel time course study was made of the status of the sympathetic vasoconstrictor innervation to the affected hind paw (plantar artery and vein). As demonstrated with a histofluorescence method that visualizes catecholamines, there was a gradual loss of norepinephrine (NE)-containing sympathetic efferents on the nerve-injured side. The decrease was first noted on postoperative day 5 (PO5), was very marked by PO10-PO14, and progressed to a complete or nearly complete loss by PO30. There was a concommitant decrease in staining for two other substances found in vasoconstrictor efferents, dopamine-beta-hydroxylase (DBH) and neuropeptide Y (NPY). The NE-containing innervation of the contralateral (sham-operated) plantar vessels appeared to be normal at all times. Lastly, in order to determine whether there was any relation between the temperature abnormality and the status of the sympathetic perivascular plexus, additional rats were sacrificed immediately after skin temperature measurement and the hind paw vessels were stained for NE. The vasculature of some abnormally cold paws had no detectable NE. Some rats that did not appear to have a temperature abnormality also had no detectable NE on the affected hind paw's vasculature. The vasculature of some abnormally hot paws had normal NE. Although it is a common clinical assumption that the temperature abnormality that accompanies painful neuropathies is a reflection of the level of sympathetic vasomotor activity, the present results suggest that this is not necessarily true. C1 NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 30,ROOM B-20,BETHESDA,MD 20892. NR 52 TC 101 Z9 102 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3959 J9 PAIN JI Pain PD SEP PY 1991 VL 46 IS 3 BP 299 EP 313 DI 10.1016/0304-3959(91)90113-C PG 15 WC Anesthesiology; Clinical Neurology; Neurosciences SC Anesthesiology; Neurosciences & Neurology GA GH319 UT WOS:A1991GH31900007 PM 1661885 ER PT J AU STEVENS, CW KAJANDER, KC BENNETT, GJ SEYBOLD, VS AF STEVENS, CW KAJANDER, KC BENNETT, GJ SEYBOLD, VS TI BILATERAL AND DIFFERENTIAL CHANGES IN SPINAL MU-OPIOID, DELTA-OPIOID AND KAPPA-OPIOID BINDING IN RATS WITH A PAINFUL, UNILATERAL NEUROPATHY SO PAIN LA English DT Article DE INTRATHECAL OPIATES; OPIATE ANALGESIA; OPIOID RECEPTORS; PAINFUL NEUROPATHY; RECEPTOR AUTORADIOGRAPHY ID SUPERFICIAL DORSAL HORN; PERIPHERAL NEUROPATHY; RECEPTOR-BINDING; ARTHRITIC RATS; TRANSSYNAPTIC DEGENERATION; SYSTEMIC NALOXONE; AFFERENT-FIBERS; GENE-EXPRESSION; DYNORPHIN-A; CORD AB Quantitative receptor autoradiography was used to assess mu, delta and kappa opioid binding sites in the lumbar spinal cord of rats with neuropathic pain due to a unilateral chronic constriction injury (CCI) of the sciatic nerve. Sections from spinal segment L4 were obtained from animals of treatment groups (left side CCI, right side sham-operated) at 2, 5 and 10 days post surgery and from control animals (left side sham-operated, right side untreated) 10 days post surgery. Autoradiograms were made of the equilibrium binding of the highly selective opioid radioligands, H-3-sufentanil (mu ligand), H-3-[D-Pen2,5]-enkephalin (DPDPE, delta ligand) and H-3-U69593 (Upjohn compound, kappa ligand). Computerized grain counting was performed on discrete regions of the autoradiograms corresponding to areas within laminae I-II, V and X on both sides of the spinal cord; the sciatic nerve's small diameter axons terminate in these areas. With a single exception, there were no changes in binding for any of the ligands in any of the areas at 10 days post surgery in the control animals. The exception was a small increase in kappa binding in laminae I-II on the sham-operated side. After nerve injury, however, there were marked changes (compared to the sham-operated side of the control animals) in the amount of binding of all ligands, and most of these changes were bilateral. Mu binding was significantly increased 2-5 days post injury, bilateral to the injury in laminae V and X but only ipsilateral in laminae I-II. Mu binding in all laminae gradually declined towards control values. By day 10 significant differences remained only in lamina X. Delta binding displayed little change at 2 days post injury but declined gradually thereafter. By day 10 post injury, delta binding was significantly decreased in all three areas; these decreases were bilateral in all areas and approximately equal in laminae V and X but were significantly greater on the nerve-injured side in laminae I-II. Kappa binding displayed a complex pattern of changes at day 2 post injury: a significant increase in ipsilateral laminae I-II and a significant increase in contralateral lamina X but no change on either side in lamina V. There was a rapid decrease in kappa binding in all three areas on both sides of the spinal cord by day 5 post injury, and these decreases were little changed by day 10. At day 5 post injury, these bilateral decreases were approximately equal in all three areas, but at day 10 the decrease in lamina X was significantly greater on the nerve-injured side. The effects on opioid binding may be due to alterations in synaptic activity evoked by spontaneous discharges in primary afferents from the injured nerve, to activity in intraspinal circuitry, or to a pain- or stress-evoked activation of descending pathways. Our observations suggest that rats, and perhaps people, with painful peripheral neuropathies may have altered responses to opiate analgesics, especially for opiates given intrathecally. C1 UNIV MINNESOTA,DEPT CELL BIOL & NEUROANAT,4-135 JACKSON HALL,MINNEAPOLIS,MN 55455. NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BETHESDA,MD 20892. FU NINDS NIH HHS [NS17702]; PHS HHS [07234] NR 84 TC 102 Z9 104 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3959 J9 PAIN JI Pain PD SEP PY 1991 VL 46 IS 3 BP 315 EP 326 DI 10.1016/0304-3959(91)90114-D PG 12 WC Anesthesiology; Clinical Neurology; Neurosciences SC Anesthesiology; Neurosciences & Neurology GA GH319 UT WOS:A1991GH31900008 PM 1661886 ER PT J AU DUBNER, R AF DUBNER, R TI NMDA RECEPTORS AND CENTRAL HYPERALGESIC STATES - REPLY SO PAIN LA English DT Letter ID RAT RP DUBNER, R (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BETHESDA,MD 20892, USA. NR 6 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3959 J9 PAIN JI Pain PD SEP PY 1991 VL 46 IS 3 BP 346 EP 346 DI 10.1016/0304-3959(91)90119-I PG 1 WC Anesthesiology; Clinical Neurology; Neurosciences SC Anesthesiology; Neurosciences & Neurology GA GH319 UT WOS:A1991GH31900013 ER PT J AU KIDOWAKI, T THIELE, CJ KLEINMAN, HK ISRAEL, MA AF KIDOWAKI, T THIELE, CJ KLEINMAN, HK ISRAEL, MA TI MATRIX PROTEINS INDUCE NEUROBLASTOMA CELL-DIFFERENTIATION WITHOUT ALTERING CELL-GROWTH SO PATHOBIOLOGY LA English DT Article DE EXTRACELLULAR MATRIX; DIFFERENTIATION; NEUROBLASTOMA; RETINOIC ACID; GROWTH REGULATION ID HUMAN NEURO-BLASTOMA; PERIPHERAL NERVOUS-SYSTEM; SUBSTRATUM-BOUND FIBRONECTIN; EXTRACELLULAR-MATRIX; RETINOIC ACID; CREST CELLS; BASEMENT-MEMBRANES; MOLECULAR-CLONING; I CDNA; LAMININ AB The maturation of embryonal neural crest cells is thought to be regulated in part by the milieu into which these cells migrate. Neuroblastoma (NB) is a tumor of very early childhood that is thought to arise in association with the arrested differentiation of embryonal neural crest cells. In culture, neuroblastoma tumor cells differentiate in the presence of retinoic acid, which is also known to influence extracellular matrix protein synthesis. We have cultured neuroblastoma cells on laminin (LN) and fibronectin (FN) substrata to examine the role of extracellular matrix in retinoic acid (RA)-induced differentiation of these tumor cells. These proteins caused morphologic changes in NB cells indistinguishable from those caused by RA. Antiserum to each of these proteins blocked the effects induced by the corresponding protein, but neither antiserum affected the action of RA. Despite the induction of a neuronal morphologic change, matrix proteins did not alter the proliferation of NB cells. These results indicate that LN and FN modulate the differentiation of NB cells without inducing growth arrest and that RA-induced differentiation does not require these matrix proteins. C1 NIDR,BETHESDA,MD 20892. NCI,BETHESDA,MD 20892. NR 53 TC 7 Z9 7 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1015-2008 J9 PATHOBIOLOGY JI Pathobiology PD SEP-OCT PY 1991 VL 59 IS 5 BP 316 EP 323 DI 10.1159/000163670 PG 8 WC Cell Biology; Pathology SC Cell Biology; Pathology GA FV916 UT WOS:A1991FV91600003 PM 1910526 ER PT J AU SWEDO, SE RETTEW, DC KUPPENHEIMER, M LUM, D DOLAN, S GOLDBERGER, E AF SWEDO, SE RETTEW, DC KUPPENHEIMER, M LUM, D DOLAN, S GOLDBERGER, E TI CAN ADOLESCENT SUICIDE ATTEMPTERS BE DISTINGUISHED FROM AT-RISK ADOLESCENTS SO PEDIATRICS LA English DT Article DE SUICIDE ATTEMPT; RISK FACTORS; ADOLESCENCE ID BEHAVIOR; CHILDREN; HOPELESSNESS; DEPRESSION; PARASUICIDE; PREVALENCE; LUNDBY; INTENT AB Psychiatric disorders, psychosocial dysfunction, family pathology, and environmental stressors are thought to be risk factors for adolescent suicide attempts. Variables from each of these categories were examined, by means of a structured interview and questionnaires, to determine whether a group of 21 adolescent suicide attempters could be differentiated from a group of 34 normal control subjects and a group of 15 at-risk adolescents (teenagers with known risk factors but without recent suicide attempt). The attempters differed significantly from control subjects on a large number of variables, particularly in the areas of substance abuse, depression, self-image, interpersonal relationships, communication patterns, family support, and problem behaviors. Only three items -the Beck Hopelessness Scale score, the SCL-90-R Positive Symptom Distress Index, and a history of suicidal ideation-differentiated the attempters from the at-risk adolescents. A discriminant analysis revealed that hopelessness and suicidal ideation were able to identify 93% of the suicide attempters. C1 NORTHWESTERN UNIV,EVANSTON HOSP,DEPT PEDIAT,EVANSTON,IL 60201. MICHAEL REESE HOSP & MED CTR,CTR STUDY ADOLESCENTS,CHICAGO,IL 60616. RP SWEDO, SE (reprint author), NIMH,CHILD PSYCHIAT BRANCH,BLDG 10,ROOM 6N240,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 84 TC 32 Z9 34 U1 5 U2 8 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD, ELK GROVE VILLAGE, IL 60007-1098 SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD SEP PY 1991 VL 88 IS 3 BP 620 EP 629 PG 10 WC Pediatrics SC Pediatrics GA GF577 UT WOS:A1991GF57700030 PM 1881745 ER PT J AU MALIN, DH LAKE, JR LEYVA, JE HAMMOND, MV ROGILLIO, RB ARCANGELI, KR LUDGATE, K MOORE, GM PAYZA, K AF MALIN, DH LAKE, JR LEYVA, JE HAMMOND, MV ROGILLIO, RB ARCANGELI, KR LUDGATE, K MOORE, GM PAYZA, K TI ANALOG OF NEUROPEPTIDE-FF ATTENUATES MORPHINE ABSTINENCE SYNDROME SO PEPTIDES LA English DT Article DE MORPHINE; OPIATE DEPENDENCE; OPIATE ABSTINENCE SYNDROME; F8FAMIDE; NEUROPEPTIDE-FF; MORPHINE-MODULATING PEPTIDE; FMRFAMIDE; ANTIOPIATE PEPTIDES ID RAT SPINAL-CORD; RECEPTORS; IMMUNOREACTIVITY; FRAGMENTS; PEPTIDES; ACINI AB The octapeptide FLFQPQRFamide (neuropeptide FF or F8Fa) may play a role in opiate dependence and subsequent abstinence syndrome. Previously, NPFF precipitated opiate abstinence syndrome, while IgG from NPFF antiserum attenuated subsequent naloxone-precipitated abstinence signs in dependent rats. The peptide desaminoYFLFQPQRamide (daY8Ra) was synthesized as a possible NPFF antagonist. At a dose of 600 ng ICV, daY8Ra significantly attenuated (p < 0.001) the number of abstinence-like signs subsequently induced by 10-mu-g NPFF ICV, suggesting that daY8Ra does have antagonist activity against NPFF. Pretreatment of morphine-dependent rats with the same dose of daY8Ra also significantly attenuated (p < 0.001) the abstinence signs subsequently precipitated by 10-mu-g naloxone ICV. Pretreatment with 600 ng of NPFF itself, or of NPFF modified at the N-terminal only (daY9Fa), failed to attenuate subsequent naloxone-precipitated abstinence, suggesting that the C-terminal modification is critical for NPFF antagonist activity. It should be noted, however, that higher doses of daY8Ra (2-mu-g or more) can precipitate some abstinence signs in a manner similar to NPFF. C1 NICHHD, DEV NEUROBIOL LAB, BETHESDA, MD 20892 USA. RP MALIN, DH (reprint author), UNIV HOUSTON CLEAR LAKE, BOX 237, 2700 BAY AREA BLVD, HOUSTON, TX 77058 USA. NR 16 TC 49 Z9 50 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 J9 PEPTIDES JI Peptides PD SEP-OCT PY 1991 VL 12 IS 5 BP 1011 EP 1014 DI 10.1016/0196-9781(91)90052-Q PG 4 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA GM636 UT WOS:A1991GM63600018 PM 1800944 ER PT J AU PITHA, J MALLIS, LM LAMB, DJ IRIE, T UEKAMA, K AF PITHA, J MALLIS, LM LAMB, DJ IRIE, T UEKAMA, K TI CYCLODEXTRIN SULFATES - CHARACTERIZATION AS POLYDISPERSE AND AMORPHOUS MIXTURES SO PHARMACEUTICAL RESEARCH LA English DT Article DE CYCLODEXTRIN SULFATES; HYDROXYPROPYLCYCLODEXTRIN SULFATES; AMORPHOUS CYCLODEXTRIN DERIVATIVES; MASS SPECTROMETRY ID NUCLEAR MAGNETIC-RESONANCE; MASS-SPECTROMETRY; INCLUSION AB Alpha- and beta-cyclodextrins and their hydroxypropyl derivatives were converted by the reaction with chlorosulfonic acid in pyridine to the corresponding sulfates. Cyclodextrin sulfates were shown by fast-atom bombardment mass spectrometry (negative ion mode, triethanolamine matrix) to be mixtures with nearly symmetrical distributions of degree of substitution by sulfate groups and by powder X-ray diffraction to be amorphous. Thus, in these aspects, cyclodextrin sulfates are similar to the potent drug solubilizers hydroxypropylcyclodextrins. C1 UNIV IOWA,HIGH RESOLUT MASS SPECTROMETRY FACIL,IOWA CITY,IA 52242. KUMAMOTO UNIV,FAC PHARMACEUT SCI,KUMAMOTO 862,JAPAN. RP PITHA, J (reprint author), NIA,GERIATR RES CTR,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. RI Uekama, Kaneto/G-9890-2011 NR 21 TC 21 Z9 21 U1 0 U2 3 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0724-8741 J9 PHARMACEUT RES JI Pharm. Res. PD SEP PY 1991 VL 8 IS 9 BP 1151 EP 1154 DI 10.1023/A:1015854402122 PG 4 WC Chemistry, Multidisciplinary; Pharmacology & Pharmacy SC Chemistry; Pharmacology & Pharmacy GA GD324 UT WOS:A1991GD32400010 PM 1788160 ER PT J AU COHEN, C WELZL, H BATTIG, K AF COHEN, C WELZL, H BATTIG, K TI EFFECTS OF NICOTINE, CAFFEINE, AND THEIR COMBINATION ON LOCOMOTOR-ACTIVITY IN RATS SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Article DE NICOTINE; CAFFEINE; DRUG INTERACTIONS; LOCOMOTOR ACTIVITY; TUNNEL MAZE; TOLERANCE; RAT ID TOLERANT RATS; EXPOSURE AB The interactive effect of caffeine and nicotine on spontaneous locomotor activity in a tunnel maze was determined in nicotine-naive and nicotine-tolerant rats. Rats were daily injected subcutaneously for 12 days with nicotine (0.4 mg/kg) to induce nicotine tolerance. Nicotine-naive rats were injected with saline. During the next two days, they were exposed to a tunnel maze for two 6-min trials. On the third day, locomotor activity was measured (30-min trial) in the tunnel maze 15 minutes after subcutaneous injection of saline, nicotine (0.2 mg/kg), caffeine (8 mg/kg), or nicotine (0.2 mg/kg) and caffeine (8 mg/kg) in combination. Acute exposure to nicotine decreased locomotor activity in nicotine-naive rats. This decrease was antagonized by simultaneous injection of caffeine. Chronic nicotine exposure induced the development of tolerance to the acute behavioral depressive effects of nicotine. In nicotine-tolerant rats, caffeine and nicotine in combination significantly increased locomotor activity above saline level, whereas given alone they had no significant stimulant effect. Neither chronic nicotine treatment nor acute drug treatments affected exploratory efficiency of rats. C1 SWISS FED INST TECHNOL,BEHAV BIOL LAB,TURNERSTR 1,CH-8092 ZURICH,SWITZERLAND. NIDA,ADDICT RES CTR,BALTIMORE,MD 21224. NR 16 TC 18 Z9 18 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD SEP PY 1991 VL 40 IS 1 BP 121 EP 123 DI 10.1016/0091-3057(91)90331-U PG 3 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA GN106 UT WOS:A1991GN10600020 PM 1780334 ER PT J AU LIPSKA, BK JASKIW, GE WEINBERGER, DR AF LIPSKA, BK JASKIW, GE WEINBERGER, DR TI DORSAL HIPPOCAMPAL LESION ABOLISHES THE RESPONSE TO FG-7142 IN THE RAT SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Note DE IBOTENIC ACID LESION; DORSAL HIPPOCAMPUS; VENTRAL HIPPOCAMPUS; FG-7142; LOCOMOTION; EXPLORATION ID MEDIAN RAPHE NUCLEI; BETA-CARBOLINE; BENZODIAZEPINE RECEPTORS; PREFRONTAL CORTEX; CEREBRAL-CORTEX; DOPAMINE; ACCUMBENS; TERMINALS; EFFICACY; BEHAVIOR AB The effects of the anxiogenic beta-carboline FG-7142 (15 mg/kg IP) on exploratory locomotor activity were assessed in rats with sham or ibotenic acid (IA) lesions of the dorsal or ventral hippocampus. FG-7142 reduced exploratory activity similarly in control animals as well as in those with IA lesions of the ventral hippocampus. In contrast, FG-7142 had no effect on rats with dorsal hippocampal lesions. The results suggest that the dorsal hippocampus plays a unique role in FG-7142-mediated attenuation of locomotor exploration. Other studies suggest that serotonergic systems may mediate these properties of FG-7142. RP LIPSKA, BK (reprint author), ST ELIZABETH HOSP,CTR NEUROSCI,NIMH,INTRAMURAL RES PROGRAM,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032, USA. NR 27 TC 10 Z9 10 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD SEP PY 1991 VL 40 IS 1 BP 169 EP 172 DI 10.1016/0091-3057(91)90339-4 PG 4 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA GN106 UT WOS:A1991GN10600028 PM 1723526 ER PT J AU LIPSKA, BK JASKIW, GE KAROUM, F PHILLIPS, I KLEINMAN, JE WEINBERGER, DR AF LIPSKA, BK JASKIW, GE KAROUM, F PHILLIPS, I KLEINMAN, JE WEINBERGER, DR TI DORSAL HIPPOCAMPAL LESION DOES NOT AFFECT DOPAMINERGIC INDEXES IN THE BASAL GANGLIA SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Note DE DORSAL HIPPOCAMPUS; DOPAMINE; IBOTENIC ACID; AMPHETAMINE; LOCOMOTOR RESPONSE; NUCLEUS ACCUMBENS ID MEDIAL PREFRONTAL CORTEX; IBOTENIC ACID LESIONS; NUCLEUS-ACCUMBENS; RAT-BRAIN; SCHIZOPHRENIA; PROJECTIONS; TERMINALS; NEURONS; STRESS AB To determine the influence of intrinsic neurons of the dorsal hippocampus on dopamine (DA) turnover in other limbic areas, DA and its metabolites were assayed in several brain areas 14 and 28 days after bilateral ibotenic acid (IA) lesions of the dorsal hippocampus in the rat. The locomotor response to d-amphetamine was also assessed. Spontaneous locomotion was increased 14 but not 28 days postoperatively. There was no change in d-amphetamine-induced locomotion at any time. Presynaptic indices of DA turnover in the medial prefrontal cortex, anteromedial striatum and nucleus accumbens were not affected by the lesion. Unlike lesions of the medial prefrontal cortex, deefferentation of the dorsal hippocampus does not increase DA turnover in the basal ganglia. C1 ST ELIZABETH HOSP,CTR NEUROSCI,NIMH,INRAMURAL RES PROGRAM,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. RP LIPSKA, BK (reprint author), ST ELIZABETH HOSP,CTR NEUROSCI,CLIN BRAIN DISORDERS BRANCH,WAW BLDG,ROOM 500,WASHINGTON,DC 20032, USA. NR 25 TC 21 Z9 21 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD SEP PY 1991 VL 40 IS 1 BP 181 EP 184 DI 10.1016/0091-3057(91)90342-Y PG 4 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA GN106 UT WOS:A1991GN10600031 PM 1664108 ER PT J AU ANDERSON, LM JONES, AB RIGGS, CW AF ANDERSON, LM JONES, AB RIGGS, CW TI LONG-TERM (IMPRINTING) EFFECTS OF TRANSPLACENTAL TREATMENT OF MICE WITH 3-METHYLCHOLANTHRENE OR BETA-NAPHTHOFLAVONE ON HEPATIC-METABOLISM OF 3-METHYLCHOLANTHRENE SO PHARMACOLOGY & TOXICOLOGY LA English DT Article ID HYDROXYLASE-ACTIVITY; PERINATAL EXPOSURE; PRENATAL EXPOSURE; RAT-LIVER; ADULT-RAT; MONOOXYGENASE; SYSTEM; INDUCTION; INDUCERS; GENOTYPE AB Foetal mice of genotype Ah(b)Ah(d) (responsive to induction of metabolism of polycyclic aromatic hydrocarbons [PAH]) or Ah(d)Ah(d) (non-responsive) were exposed transplacentally on gestation day 17 to a single dose of 3-methylcholanthrene (MC, 5-175 mg/kg) with or without prior treatment on day 15 with beta-naphthoflavone (beta-NF, 150 mg/kg). The mothers were themselves either induction-responsive [(C57BL/6 x DBA/2)F1] or non-responsive (DBA/2). Metabolism of [C-14]MC by homogenates of livers from the transplacentally-exposed offspring was quantified at 9 months of age (first experiment) or 13 months (second experiment) with or without prior inducing treatment with MC. The foetal exposure to MC had a permanent effect on MC metabolism by the adult hepatic homogenates in both experiments. In most instances the effect was positive in direction and small in magnitude (15-30%). It was dose-dependent with regard to transplacental MC, occurred in both induced (Ah(b)Ah(d)) and non-induced (Ah(d)Ah(d)) individuals, and was significant only when the mother and/or the foetus was inducible. Beta-NF itself did not have a positive imprinting effect. In some cases it either reduced or potentiated the long-term imprinting effect of MC, depending on the MC dose and the phenotype of the mother. These results confirm that transplacental exposure to a carcinogenic PAH may permanently alter metabolism of the chemical in later life. and indicate that this imprinting action is dependent on induced metabolism of the chemical in the mother and/or foetus. C1 NCI,FREDERICK CANC RES & DEV CTR,DATA MANAGEMENT SERV INC,FREDERICK,MD 21701. RP ANDERSON, LM (reprint author), NCI,FREDERICK CANC RES & DEV CTR,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21701, USA. NR 30 TC 16 Z9 16 U1 0 U2 0 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0901-9928 J9 PHARMACOL TOXICOL JI Pharmacol. Toxicol. PD SEP PY 1991 VL 69 IS 3 BP 178 EP 188 PG 11 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA GF081 UT WOS:A1991GF08100006 PM 1796059 ER PT J AU BILSKI, P LI, ASW CHIGNELL, CF AF BILSKI, P LI, ASW CHIGNELL, CF TI THE PHOTOOXIDATION OF N,N-DIETHYLHYDROXYLAMINE BY ROSE-BENGAL IN ACETONITRILE AND WATER SO PHOTOCHEMISTRY AND PHOTOBIOLOGY LA English DT Article ID SINGLET MOLECULAR-OXYGEN; SUPEROXIDE ION; FREE-RADICALS; NITROXIDE; SPECTROSCOPY; GENERATION; RESONANCE; SYSTEMS; ANION; IRON AB The Rose Bengal photosensitized oxidation of N,N-diethylhydroxylamine has been investigated in water and acetonitrile using the techniques of oxygen uptake, singlet oxygen phosphorescence and electron spin resonance. In both solvents H2O2 is the major oxidation product and diethylnitroxide is an intermediate. In water, superoxide dismutase decreases oxygen uptake suggesting involvement of superoxide anions in the oxidation process. Results indicate that in water the photo-oxidation proceeds mainly by a Type I (electron transfer) mechanism, while in acetonitrile a Type II (energy transfer) mechanism has been confirmed (Encinas et al., 1987, J. Chem. Soc. Perkin Trans. II, 1125-1127). RP BILSKI, P (reprint author), NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 26 TC 20 Z9 20 U1 0 U2 2 PU AMER SOC PHOTOBIOLOGY PI AUGUSTA PA BIOTECH PARK, 1021 15TH ST, SUITE 9, AUGUSTA, GA 30901-3158 SN 0031-8655 J9 PHOTOCHEM PHOTOBIOL JI Photochem. Photobiol. PD SEP PY 1991 VL 54 IS 3 BP 345 EP 352 DI 10.1111/j.1751-1097.1991.tb02026.x PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GA539 UT WOS:A1991GA53900003 ER PT J AU TALAN, MI TATELMAN, HM ENGEL, BT AF TALAN, MI TATELMAN, HM ENGEL, BT TI COLD TOLERANCE AND METABOLIC HEAT-PRODUCTION IN MALE C57BL/6J MICE AT DIFFERENT TIMES OF DAY SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE COLD EXPOSURE; BODY TEMPERATURE; DIURNAL; HABITUATION; THERMOGENESIS ID BLOOD-FLOW; OXYGEN-CONSUMPTION; SQUIRREL-MONKEY; LABORATORY RATS; PATTERNS; RHYTHM; STRESS AB Nine-month-old male C57BL/6J mice were subjected to three-hour cold stress tests (partial restraint at 6-degrees-C) at 9:00 a.m. or at 1:00 p.m. Tests were repeated three times at two-week intervals at the same time of day. Body temperature was measured by colonic thermoprobe, and metabolic heat production was measured by indirect calorimetry during each test. All mice showed habituation to repeated cold exposures (an improvement of cold tolerance across tests) due to an increase in metabolic heat production. The levels of metabolic heat production were similar during morning and afternoon testing; however, mice tested in the afternoon had consistently poorer cold tolerance, which indicated increased heat loss. Increased heat loss in mice of similar body weight and presumably similar body composition, suggests that there is less effective cold-induced skin vasoconstriction during the afternoon. We hypothesize that the compromised skin vasomotor response during the afternoon cold exposure results from competing effects of vasodilation due to local autoregulation stimulated by a circadian reduction of cardiac output during the sleep phase, and vasoconstriction due to the cold stress. RP TALAN, MI (reprint author), NIA,GERONTOL RES CTR,BEHAV SCI LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 30 TC 12 Z9 12 U1 0 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD SEP PY 1991 VL 50 IS 3 BP 613 EP 616 DI 10.1016/0031-9384(91)90554-2 PG 4 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA GK171 UT WOS:A1991GK17100024 PM 1801018 ER PT J AU SHULDINER, AR DEPABLO, F MOORE, CA ROTH, J AF SHULDINER, AR DEPABLO, F MOORE, CA ROTH, J TI 2 NONALLELIC INSULIN GENES IN XENOPUS-LAEVIS ARE EXPRESSED DIFFERENTIALLY DURING NEURULATION IN PREPANCREATIC EMBRYOS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE XENOPUS INSULIN; NEUROGENESIS; OOGENESIS; GROWTH FACTORS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION ID AMINO-ACID-SEQUENCE; GROWTH FACTOR-I; PROTHORACICOTROPIC HORMONE; PROTEIN-SYNTHESIS; NERVOUS-SYSTEM; OOCYTES; INVITRO; NEURONS; SEGMENTATION; NEUROGENESIS AB Insulin, traditionally regarded as a metabolic hormone, also can potently stimulate growth and differentiation in many cell types. To study further the potential role of insulin during early embryogenesis, we have used the amphibian Xenopus laevis, a versatile model of vertebrate development. Using (i) nucleotide sequences of two previously cloned cDNAs that correspond to two different nonallelic Xenopus insulin genes (both of which are expressed in the adult pancreas) and (ii) a modification of the highly sensitive reverse transcription-polymerase chain reaction (RT-PCR) method developed in our laboratory, designated RNA template-specific PCR (RS-PCR), we now rind that mRNAs for both Xenopus insulins I and II are present in mature (stage VI) oocytes but not in less-mature oocytes (stages I and IV) or in unfertilized eggs. The Xenopus insulin II gene is differentially expressed during early neurulation (stage 13), while only the insulin I gene is expressed at stage 21, when the neural tube is closing and cephalization is beginning. During later stages (i.e., stage 26) there is a region in the head that appears to be transcribing only the insulin I gene, while mRNAs for both insulins I and II are present in the body region. These findings show that the two nonallelic insulin genes are expressed differentially in Xenopus embryos in a stage- and region-specific manner; because appropriate receptors are also present, we suggest a role for insulin during early nervous system development well before the emergence of pancreatic beta-cells. RP SHULDINER, AR (reprint author), NIDDKD,DIABET BRANCH,RECEPTORS & HORMONE ACT,BLDG 10,ROOM 8-S-243,BETHESDA,MD 20892, USA. NR 50 TC 48 Z9 48 U1 1 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP PY 1991 VL 88 IS 17 BP 7679 EP 7683 DI 10.1073/pnas.88.17.7679 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GC992 UT WOS:A1991GC99200047 PM 1715574 ER PT J AU GESSAIN, A YANAGIHARA, R FRANCHINI, G GARRUTO, RM JENKINS, CL AJDUKIEWICZ, AB GALLO, RC GAJDUSEK, DC AF GESSAIN, A YANAGIHARA, R FRANCHINI, G GARRUTO, RM JENKINS, CL AJDUKIEWICZ, AB GALLO, RC GAJDUSEK, DC TI HIGHLY DIVERGENT MOLECULAR VARIANTS OF HUMAN T-LYMPHOTROPIC VIRUS TYPE-I FROM ISOLATED POPULATIONS IN PAPUA-NEW-GUINEA AND THE SOLOMON-ISLANDS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE RETROVIRUS; PCR; MELANESIA; EVOLUTION ID CELL LEUKEMIA-VIRUS; COMPLETE NUCLEOTIDE-SEQUENCE; TROPICAL SPASTIC PARAPARESIS; HTLV-I; HIGH PREVALENCE; INFECTION; ANTIBODIES; RETROVIRUS; MYELOPATHY; PACIFIC AB To determine the molecular genetic relationship between Melanesian strains of human T-lymphotropic virus type I (HTLV-I) and cosmopolitan prototype HTLV-I, we amplified by PCR, then cloned, and sequenced a 522-base-pair region of the HTLV-I env gene in DNA extracted from uncultured (fresh) and cultured peripheral blood mononuclear cells obtained from six seropositive Melanesian Papua New Guineans and Solomon Islanders, including a Solomon Islander with HTLV-1 myeloneuropathy. Unlike isolates of HTLV-I from Japan, the West Indies, the Americas, and Africa, which share greater-than-or-equal-to 97% sequence homology, the Melanesian strains of HTLV-I were only 91.8%-92.5% identical with a prototype Japanese HTLV-I(ATK-1). The nucleotide sequence of proviral DNA from the Solomon Islander with HTLV-I myeloneuropathy also diverged markedly from that of HTLV-I isolated from Japanese patients with HTLV-I-associated myelopathy and from Jamaican patients with tropical spastic paraparesis, suggesting that these variant viruses are capable of causing disease. The HTLV-l variants from Papua New Guineans, in turn, differed by nearly 4% from the Melanesian variants from Solomon Islanders, indicating the existence of another HTLV-I quasispecies. By contrast, HTLV-1 strains from two residents of Bellona Island, a Polynesian Outlier within the Solomon Islands, were closely related to cosmopolitan prototype HTLV-I (greater-than-or-equal-to 97% sequence identity), suggesting recent introduction, possibly during this century. These findings are consistent with a proto-Melanesian HTLV-I strain of archaic presence, which evolved independently of contemporary cosmopolitan strains, and pose new questions about the origin and global dissemination of HTLV-I. C1 NINCDS,CENT NERVOUS SYST STUDIES LAB,BETHESDA,MD 20892. NCI,TUMOR CELL BIOL LAB,BETHESDA,MD 20892. CENT HOSP,MINIST HLTH & MED SERV,HONIARA,SOLOMON ISLANDS. PAPUA NEW GUINEA INST MED RES,GOROKA,PAPUA N GUINEA. NR 38 TC 124 Z9 125 U1 1 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP PY 1991 VL 88 IS 17 BP 7694 EP 7698 DI 10.1073/pnas.88.17.7694 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GC992 UT WOS:A1991GC99200050 PM 1881912 ER PT J AU BRINDLEY, PJ HEATH, S WATERS, AP MCCUTCHAN, TF SHER, A AF BRINDLEY, PJ HEATH, S WATERS, AP MCCUTCHAN, TF SHER, A TI CHARACTERIZATION OF A PROGRAMMED ALTERATION IN AN 18S-RIBOSOMAL GENE THAT ACCOMPANIES THE EXPERIMENTAL INDUCTION OF DRUG-RESISTANCE IN SCHISTOSOMA-MANSONI SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE SCHISTOSOMES; HYCANTHONE; RIBOSOMAL-RNA-ENCODING DNA ID HYCANTHONE; DNA; PLASMODIUM; SEQUENCES; GENOME; MODE AB Stable resistance to the anthelmintic hycanthone can be produced in the human blood fluke Schistosoma mansoni by exposing immature parasites in mice to the drug. Within a single generation, genomic rearrangements, detected as rRNA-encoding DNA restriction fragment length polymorphisms (RFLPs), accompany% the appearance of resistance in this model. One of these RFLPs, an almost-equal-to 3.6-kilobase BamHI fragment, was shown previously to associate consistently with resistance in independent generations of the JHU strain of S. mansoni. To characterize the genetic changes responsible for this RFLP, the fragment was cloned and sequenced. A comparison of the cloned fragment with a normal 18S rRNA gene demonstrated that the drug resistance-associated RFLP fragment arises through the addition of 732 base pairs into an 18S rRNA gene, 134 base pairs downstream of the junction of the intergenic spacer and the mature 18S rRNA gene. The mutation is nonrandom, targets one, or a few only, of the 100 or so copies of the ribosomal genes, and may represent the incomplete duplication of the gene since the inserted element is identical in sequence to the region contiguous to it. The sequence spanning the junction of the insertion and the original 18S rRNA gene was used as a specific primer for the BamHI RFLP in PCR experiments. The analysis conclusively demonstrated that the mutation is induced rather than selected by the drug since the junctional sequence was not detectable in the drug-sensitive parent population of schistosomes. In addition, analysis of four, independently derived, resistant lines indicated that the same region of the gene was mutated each time. Together, these data demonstrate that reproducible changes are induced during the acquisition of resistance in schistosomes and suggest that the resistant phenotype is induced rather than selected from preexisting forms. RP BRINDLEY, PJ (reprint author), NIAID,PARASIT DIS LAB,BLDG 4,ROOM 126,BETHESDA,MD 20892, USA. RI Waters, Andy/C-9377-2009 OI Waters, Andy/0000-0001-8900-2982 NR 23 TC 21 Z9 22 U1 0 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP PY 1991 VL 88 IS 17 BP 7754 EP 7758 DI 10.1073/pnas.88.17.7754 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GC992 UT WOS:A1991GC99200063 PM 1679236 ER PT J AU KOELLER, DM HOROWITZ, JA CASEY, JL KLAUSNER, RD HARFORD, JB AF KOELLER, DM HOROWITZ, JA CASEY, JL KLAUSNER, RD HARFORD, JB TI TRANSLATION AND THE STABILITY OF MESSENGER-RNAS ENCODING THE TRANSFERRIN RECEPTOR AND C-FOS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE IRON; FERRITIN; PROTEIN SYNTHESIS; CYCLOHEXIMIDE; PUROMYCIN ID 3' UNTRANSLATED REGION; GENE-EXPRESSION; IRON; DEGRADATION; SEQUENCES; PROTEIN; POLY(A); MYC; FERRITIN; ONCOGENE AB Turnover of the full-length human transferrin receptor (TfR) mRNA is regulated by iron, and this regulation is mediated by the transcript's 3' untranslated region. Alterations in the sequence of the TfR mRNA regulatory region have been identified that render the mRNA unregulated by iron and intrinsically unstable. When cells expressing this unstable mRNA are treated with inhibitors of protein synthesis (cycloheximide or puromycin), the steady-state level of the encoded human TfR mRNA is increased due to a stabilization of the transcript. A similar set of observations has been made using a chimeric mRNA in which the rapid turnover determinant of the TfR mRNA is replaced by the (A + U)-rich region from the 3' untranslated region of c-fos mRNA. To distinguish between a labile protein participant in the degradation of these mRNAs and a requirement for their translation per se, we introduced a ferritin iron-responsive element into the 5' untranslated region of each of these mRNAs. The presence of the 5' iron-responsive element allowed us to use iron availability to alter the translation of the mRNAs in question without global effects on cellular protein synthesis. Although specific translation of these mRNAs could be inhibited by iron chelation to a degree comparable to that seen with cycloheximide (almost-equal-to 95% inhibition), no effects on mRNA turnover were observed. These data support a model in which a trans-acting labile protein is necessary for the turnover of these mRNAs rather than there being a requirement for the translation of the mRNAs themselves. C1 NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892. NR 28 TC 119 Z9 119 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP PY 1991 VL 88 IS 17 BP 7778 EP 7782 DI 10.1073/pnas.88.17.7778 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GC992 UT WOS:A1991GC99200068 PM 1909029 ER PT J AU CIARDIELLO, F KIM, N SAEKI, T DONO, R PERSICO, MG PLOWMAN, GD GARRIGUES, J RADKE, S TODARO, GJ SALOMON, DS AF CIARDIELLO, F KIM, N SAEKI, T DONO, R PERSICO, MG PLOWMAN, GD GARRIGUES, J RADKE, S TODARO, GJ SALOMON, DS TI DIFFERENTIAL EXPRESSION OF EPIDERMAL GROWTH FACTOR-RELATED PROTEINS IN HUMAN COLORECTAL TUMORS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE COLON CANCER; MESSENGER RNA EXPRESSION; CRIPTO; AMPHIREGULIN; HER3 ID HUMAN-COLON CARCINOMA; FACTOR-ALPHA; FACTOR-RECEPTOR; GENE-MUTATIONS; MESSENGER-RNA; FACTOR-BETA; CELL-LINES; AMPHIREGULIN; CANCER; FAMILY AB Amphiregulin (AR) and cripto are proteins that are structurally related to epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha). AR is also functionally related lo this family of growth regulatory molecules and is able to bind and activate the 170-kDa EGF receptor (EGFR). Human EGFR-3 (HER3)/ERBB3 is a recently identified protein related to the EGFR that is widely expressed in breast carcinomas and is a candidate receptor for EGF-like growth factors. Differential expression or these putative ligands and receptors in transformed cells suggests that they may function in an autocrine manner to regulate tumor cell growth. Specific mRNA transcripts for TGF-alpha [4.8 kilobases (kb)], AR (1.4 kb), cripto (2.2 kb), and FIER3 (6.2 kb) were expressed in a majority of human colon cancer cell lines. HER3 mRNA was detected in 55% of primary or metastatic human colorectal carcinomas but in only 22% of normal colon mucosa and 32% of normal liver samples. In contrast, cripto and AR mRNA were expressed in 60-70% of primary or metastatic human colorectal cancers but in only 2-7% of normal human colonic mucosa. Immunostaining also detected AR protein in primary and metastatic colorectal tumors but not in normal colon or uninvolved liver. These findings suggest that cripto and AR may be useful markers to discriminate between normal and malignant colonic epithelium and may provide a selective growth advantage for colorectal carcinomas. C1 NCI,DIV CANC BIOL,TUMOR IMMUNOL & BIOL LAB,BETHESDA,MD 20892. INT INST GENET & BIOPHYS,I-80125 NAPLES,ITALY. BRISTOL MYERS SQUIBB PHARMACEUT,RES INST,SEATTLE,WA 98121. FRED HUTCHINSON CANC RES CTR,SEATTLE,WA 98104. RI PLOWMAN, Greg/E-2012-2011; DONO, ROSANNA/I-7821-2016; OI Ciardiello, Fortunato/0000-0002-3369-4841 NR 40 TC 211 Z9 219 U1 0 U2 5 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP PY 1991 VL 88 IS 17 BP 7792 EP 7796 DI 10.1073/pnas.88.17.7792 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GC992 UT WOS:A1991GC99200071 PM 1715580 ER PT J AU ZABLOCKI, K MILLER, SPF GARCIAPEREZ, A BURG, MB AF ZABLOCKI, K MILLER, SPF GARCIAPEREZ, A BURG, MB TI ACCUMULATION OF GLYCEROPHOSPHOCHOLINE (GPC) BY RENAL-CELLS - OSMOTIC REGULATION OF GPC - CHOLINE PHOSPHODIESTERASE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE NACL; UREA; PHOSPHOLIPASE-A2; MDCK CELLS; OSMOLYTES; GLYCERYL PHOSPHORYL CHOLINE ID PHOSPHATIDYLCHOLINE METABOLISM; PHOSPHOLIPID-METABOLISM; INNER MEDULLA; RAT; OSMOLYTES; GROWTH AB Although GPC has long been recognized as a degradation product of phosphatidylcholine, only recently is there wide appreciation of its role as a compatible and counteracting osmolyte that protects cells from osmotic stress. GPC is osmotically regulated in renal cells. Its level varies directly with extracellular osmolality. Cells in the kidney medulla in vivo and in renal epithelial cell cultures (MDCK) accumulate large amounts of GPC when exposed to high concentrations of NaCl and urea. Osmotic regulation of GPC requires choline in the medium, presumably as a precursor for synthesis of GPC. Choline transport into the cells, however, is not osmoregulated. The purpose of the present studies was to use MDCK cell cultures as a defined model to distinguish whether osmotically induced accumulation of GPC results from increased GPC synthesis or decreased GPC disappearance. The rate of incorporation of C-14 from [C-14]choline into GPC, the steady-state GPC synthesis rate, and the activity of phospholipase A2 (which can catalyze a step in the synthesis of GPC from phosphatidylcholine) are not increased by high NaCl and urea. In fact all are decreased by approximately one-third. Therefore, we find no evidence that high NaCl and urea increases the GPC synthesis rate. On the other hand, the rate coefficient for cellular GPC disappearance and the activity of GPC:choline phosphodiesterase (EC 3.1.4.2), which catalyzes degradation of GPC, are decreased by approximately two-thirds by high NaCl and urea. We conclude that high NaCl and urea increase the level of GPC by inhibiting its enzymatic degradation. C1 NINCDS,DEV & METAB NEUROL BRANCH,BETHESDA,MD 20892. RP ZABLOCKI, K (reprint author), NHLBI,KIDNEY & ELECTROLYTE METAB LAB,BETHESDA,MD 20892, USA. NR 30 TC 80 Z9 83 U1 0 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP PY 1991 VL 88 IS 17 BP 7820 EP 7824 DI 10.1073/pnas.88.17.7820 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GC992 UT WOS:A1991GC99200077 PM 1652765 ER PT J AU WICKNER, S HOSKINS, J MCKENNEY, K AF WICKNER, S HOSKINS, J MCKENNEY, K TI MONOMERIZATION OF REPA DIMERS BY HEAT-SHOCK PROTEINS ACTIVATES BINDING TO DNA-REPLICATION ORIGIN SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE DNAJ; DNAK; PLASMID P1 ID P1 PLASMID REPLICATION; ESCHERICHIA-COLI; NUCLEOPROTEIN STRUCTURES; BACTERIOPHAGE-LAMBDA; INITIATOR PROTEIN; GENE; DEFECTS; GRPE AB DnaK is a major heat shock protein of Escherichia coli and the homolog of hsp70 in eukaryotes. We demonstrate the mechanism by which DnaK and another heat shock protein, DnaJ, render the plasmid Pl initiator RepA 100-fold more active for binding to the PI origin of replication. Activation is the conversion of RepA dimers into monomers in an ATP-dependent reaction and the monomer form binds with high affinity to oriP1 DNA. Reversible chemical denaturants also convert RepA dimers to monomers and simultaneously activate oriP1 DNA binding. Increasing protein concentration converts monomers to dimers and deactivates RepA. Based on our data and previous work, we present a model for heat shock protein action under normal and stress conditions. C1 NATL INST STAND & TECHNOL,CTR ADV RES BIOTECHNOL,ROCKVILLE,MD 20850. RP WICKNER, S (reprint author), NCI,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 18 TC 166 Z9 167 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP PY 1991 VL 88 IS 18 BP 7903 EP 7907 DI 10.1073/pnas.88.18.7903 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GF101 UT WOS:A1991GF10100001 PM 1896443 ER PT J AU BERGER, EA LIFSON, JD EIDEN, LE AF BERGER, EA LIFSON, JD EIDEN, LE TI STIMULATION OF GLYCOPROTEIN-GP120 DISSOCIATION FROM THE ENVELOPE GLYCOPROTEIN COMPLEX OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 BY SOLUBLE CD4 AND CD4 PEPTIDE DERIVATIVES - IMPLICATIONS FOR THE ROLE OF THE COMPLEMENTARITY-DETERMINING REGION 3-LIKE REGION IN MEMBRANE-FUSION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE RECEPTOR-INDUCED STRUCTURAL CHANGE; HUMAN VS CHIMPANZEE CD4 SEQUENCE; VACCINIA VIRUS EXPRESSION VECTOR; AIDS ID RECOMBINANT VACCINIA VIRUS; SYNCYTIUM FORMATION; TRANSMEMBRANE PROTEIN; OLIGOMERIC STRUCTURE; CD4-BEARING CELLS; HIV INFECTION; BINDING-SITE; ENV PROTEIN; EXPRESSION; DOMAIN AB We have used a recombinant vaccinia virus vector encoding the envelope glycoprotein of human immunodeficiency virus type 1 to study receptor-induced structural changes related to membrane fusion. A truncated soluble form of human CD4 (sCD4) was found to stimulate dissociation of the external subunit (gp120) from the envelope glycoprotein complex of human immunodeficiency virus type 1 expressed at the cell surface. sCD4 stimulation of gp120 release was time- and concentration-dependent and was associated with specific binding of sCD4 to gp120. Synthetic peptide derivatives corresponding to residues 81-92 of human CD4 (overlapping the complementarity-determining region 3-like region) inhibited cell-cell fusion mediated by the interaction between recombinant vaccinia-encoded CD4 and human immunodeficiency virus envelope glycoprotein. These peptide derivatives also stimulated gp120 release from the envelope glycoprotein complex. An analogous peptide derivative from chimpanzee CD4 (containing a single Glu --> Gly substitution at the position corresponding to CD4 residue 87) was considerably less active at inhibition of cell-cell fusion and stimulation of gp120 release, consistent with the known inhibitory effect of this substitution on the ability of membrane-associated CD4 to mediate cell fusion. These results suggest that the sCD4-induced release of gp120 reflects postbinding structural changes in the envelope glycoprotein complex involved in membrane fusion, with the complementarity-determining region 3-like region playing a critical role. C1 NIMH,CELL BIOL LAB,BETHESDA,MD 20892. GENELABS INC,REDWOOD CITY,CA 94063. RP BERGER, EA (reprint author), NIAID,VIRAL DIS LAB,BETHESDA,MD 20892, USA. FU NIAID NIH HHS [AI25922] NR 46 TC 65 Z9 66 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP PY 1991 VL 88 IS 18 BP 8082 EP 8086 DI 10.1073/pnas.88.18.8082 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GF101 UT WOS:A1991GF10100038 PM 1896455 ER PT J AU PUTNAM, FW AF PUTNAM, FW TI RECENT RESEARCH ON MULTIPLE PERSONALITY-DISORDER SO PSYCHIATRIC CLINICS OF NORTH AMERICA LA English DT Article ID DISSOCIATIVE DISORDERS; STATES; INTERVIEW RP PUTNAM, FW (reprint author), NIMH,DEV PSYCHOL LAB,DISSOCIAT DISORDERS UNIT,BLDG 15K,BETHESDA,MD 20892, USA. NR 53 TC 30 Z9 30 U1 1 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0193-953X J9 PSYCHIAT CLIN N AM JI Psychiatr. Clin. North Amer. PD SEP PY 1991 VL 14 IS 3 BP 489 EP 502 PG 14 WC Psychiatry SC Psychiatry GA HL471 UT WOS:A1991HL47100002 PM 1946019 ER PT J AU PUTNAM, FW AF PUTNAM, FW TI DISSOCIATIVE DISORDERS IN CHILDREN AND ADOLESCENTS - A DEVELOPMENTAL PERSPECTIVE SO PSYCHIATRIC CLINICS OF NORTH AMERICA LA English DT Article ID MULTIPLE PERSONALITY-DISORDER; CHILDHOOD; VIOLENCE; PARENTS; STRESS; ABUSE RP PUTNAM, FW (reprint author), NIMH,DEV PSYCHOL LAB,DISSOCIAT DISORDERS UNIT,BLDG 15K,BETHESDA,MD 20892, USA. NR 55 TC 54 Z9 54 U1 3 U2 5 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0193-953X J9 PSYCHIAT CLIN N AM JI Psychiatr. Clin. North Amer. PD SEP PY 1991 VL 14 IS 3 BP 519 EP 531 PG 13 WC Psychiatry SC Psychiatry GA HL471 UT WOS:A1991HL47100004 PM 1946022 ER PT J AU ROSEN, LN ROSENTHAL, NE AF ROSEN, LN ROSENTHAL, NE TI SEASONAL-VARIATIONS IN MOOD AND BEHAVIOR IN THE GENERAL-POPULATION - A FACTOR-ANALYTIC APPROACH SO PSYCHIATRY RESEARCH LA English DT Article DE DEPRESSION; HYPERSOMNIA; WEIGHT CHANGE; SOCIABILITY ID AFFECTIVE-DISORDER AB In a mailed survey conducted at four centers-Nashua, NH; New York, NY; Washington, DC; and Sarasota, FL-1,671 respondents provided information on monthly variations in 10 behavioral categories representing extremes in the areas of mood, socializing, appetite, weight gain/loss, and sleep length. A 10-factor solution revealed the following factors: (1) a winter weight gain factor; (2) a winter depression factor; (3) a winter hypersomnia factor; (4) a summer weight gain factor; (5) a summer hypersomnia factor; (6) a summer depression factor; (7) a winter socializing factor; (8) a winter weight loss factor; (9) a fall depression factor; and (10) a possibly mixed factor. Factors consistent with winter seasonal affective disorder were positively correlated with latitude, while those consistent with summer seasonal affective disorder were negatively correlated with latitude. C1 NIMH,CLIN PSYCHOBIOL BRANCH,OUTPATIENT STUDIES UNIT,BLDG 10 4S-239,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. WALTER REED ARMY MED CTR,DEPT MIL PSYCHIAT,WASHINGTON,DC 20307. NR 13 TC 26 Z9 26 U1 1 U2 3 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD SEP PY 1991 VL 38 IS 3 BP 271 EP 283 DI 10.1016/0165-1781(91)90017-J PG 13 WC Psychiatry SC Psychiatry GA GM625 UT WOS:A1991GM62500006 PM 1754639 ER PT J AU CHEN, HT CASANOVA, MF KLEINMAN, JE ZITO, M GOLDMAN, D LINNOILA, M AF CHEN, HT CASANOVA, MF KLEINMAN, JE ZITO, M GOLDMAN, D LINNOILA, M TI H-3 PAROXETINE BINDING IN BRAINS OF ALCOHOLICS SO PSYCHIATRY RESEARCH LA English DT Article DE PAROXETINE BINDING; HUMAN BRAIN; ALCOHOLICS ID IMIPRAMINE BINDING; H-3 PAROXETINE; RAT-BRAIN; CITALOPRAM; COMPLEX AB High affinity H-3-paroxetine binding was studied in human frontal cortex and hippocampus obtained from normal controls and alcoholics. On the basis of Scatchard analyses, a significant decrease in the maximal number of binding sites (Bmax) was found in the hippocampus of alcoholics (n = 8) as compared with that of controls (n = 10) (mean +/- SD = 63 +/- 35 vs. 114 +/- 70 fmoles/ mg protein). There was no significant difference in the dissociation constants (Kd) between the two groups. The presumed effect of chronic alcohol abuse on H-3-paroxetine binding may be region-specific since no significant difference in either Bmax or Kd for H-3-paroxetine binding was found in the frontal cortex between normal controls and alcoholics. No significant correlation of H-3-paroxetine binding with age or post-mortem interval was observed. The decrease in H-3-paroxetine binding in the hippocampus of alcoholics is probably indicative of reduced density of serotonergic nerve terminals either as a preexisting condition or as a result of neuronal damage caused by ethanol or the sequelae of alcoholism, such as nutritional deficiencies. C1 NIAAA,DICBR,CLIN STUDIES LAB,ROCKVILLE,MD 20852. NIMH,INTRAMURAL RES PROGRAM,CLIN BRAIN DISORDERS BRANCH,BRAIN BANK UNIT,WASHINGTON,DC 20032. RI Goldman, David/F-9772-2010 OI Goldman, David/0000-0002-1724-5405 NR 22 TC 23 Z9 25 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD SEP PY 1991 VL 38 IS 3 BP 293 EP 299 DI 10.1016/0165-1781(91)90019-L PG 7 WC Psychiatry SC Psychiatry GA GM625 UT WOS:A1991GM62500008 PM 1836640 ER PT J AU KASPRZAK, KS AF KASPRZAK, KS TI ISOTOPIC AND NUCLEAR ANALYTICAL TECHNIQUES IN BIOLOGICAL-SYSTEMS A CRITICAL SURVEY - INTRODUCTION SO PURE AND APPLIED CHEMISTRY LA English DT Editorial Material RP KASPRZAK, KS (reprint author), NCI,FREDERICK CANC RES & DEV CTR,BLDG 538,ROOM 205E,FREDERICK,MD 21702, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0033-4545 J9 PURE APPL CHEM JI Pure Appl. Chem. PD SEP PY 1991 VL 63 IS 9 BP 1269 EP 1270 DI 10.1351/pac199163091269 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA GA447 UT WOS:A1991GA44700008 ER PT J AU BALABAN, RS CHESNICK, S HEDGES, K SAMAHA, F HEINEMAN, FW AF BALABAN, RS CHESNICK, S HEDGES, K SAMAHA, F HEINEMAN, FW TI MAGNETIZATION TRANSFER CONTRAST IN MR IMAGING OF THE HEART SO RADIOLOGY LA English DT Article DE HEART, EXPERIMENTAL STUDIES; HEART, MR STUDIES; MAGNETIC RESONANCE (MR), CONTRAST ENHANCEMENT; MAGNETIC RESONANCE (MR), EXPERIMENTAL; MAGNETIC RESONANCE (MR), PULSE SEQUENCES ID MOTION AB The use of magnetization transfer contrast (MTC) in short-echo-time (TE) cardiac magnetic resonance (MR) imaging was evaluated. For most cardiac MR imaging protocols, either long TE and short repetition time or exogenous intravascular agents are used for generating contrast between the ventricular wall and cavity as well as detecting pathologic conditions of the ventricular wall. The major problem with long-TE images is that the motion of the heart degrades the spatial resolution of the image during the TE period. However, MTC is generated by an off-resonance irradiation during the interpulse delay period that is relatively insensitive to motion artifacts. Short-TE (5-15 msec) gradient-recalled echo sequences were used for imaging the heart with and without MTC. These studies revealed that MTC can be used to greatly improve the contrast between the myocardial wall and blood chamber in short-TE images and may provide useful parameters for tissue characterization in pathologic cardiac muscle. RP BALABAN, RS (reprint author), NHLBI,CARDIAC ENERGET LAB,BLDG 1,RM B3-07,BETHESDA,MD 20892, USA. RI Balaban, Robert/A-7459-2009 OI Balaban, Robert/0000-0003-4086-0948 NR 25 TC 55 Z9 56 U1 0 U2 1 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD SEP PY 1991 VL 180 IS 3 BP 671 EP 675 PG 5 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA GB926 UT WOS:A1991GB92600014 PM 1871277 ER PT J AU GESSAIN, A GALLO, R AF GESSAIN, A GALLO, R TI VIRUSES AND HUMAN CANCERS SO RECHERCHE LA French DT Article ID CELL LEUKEMIA-VIRUS; HEPATOCELLULAR-CARCINOMA; GROWTH-FACTOR; T-CELLS; RETROVIRUS; ANTIBODIES C1 NCI,BIOL CELLULAIRE TUMEURS LAB,BETHESDA,MD 20892. RP GESSAIN, A (reprint author), HOP PARIS,F-75221 PARIS,FRANCE. NR 30 TC 0 Z9 0 U1 0 U2 0 PU SOC ED SCIENTIFIQUES PI PARIS 06 PA 57 RUE DE SEINE, 75280 PARIS 06, FRANCE SN 0029-5671 J9 RECHERCHE JI Recherche PD SEP PY 1991 VL 22 IS 235 BP 1036 EP 1045 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GD078 UT WOS:A1991GD07800003 ER PT J AU MARCHE, PN LAVERRIERE, A LEGUERN, C KINDT, TJ AF MARCHE, PN LAVERRIERE, A LEGUERN, C KINDT, TJ TI A GENE REMNANT IN THE RABBIT MHC RELATED TO HLA-DR-ALPHA SO RESEARCH IN IMMUNOLOGY LA English DT Article DE MHC, RABBIT, PSEUDOGENE, HLA-DR-ALPHA; DF FRAGMENT, EVOLUTION ID MAJOR HISTOCOMPATIBILITY COMPLEX; IMMUNE-RESPONSE GENE; CLASS-II GENES; D REGION; NUCLEIC-ACID; SEQUENCE; DNA; SIMILARITY; HOMOLOGY; CLONES AB The screening of a rabbit genomic library yielded 2 HLA-DR-alpha-related clones. One of these was previously shown to contain a functional gene (RLA-DR-alpha) closely related to HLA-DR-alpha. The second DR-related clone, designated DF, was mapped to the rabbit MHC class II region by genetic studies. DF contains contiguous sequences that have significant homology to the RLA-DR-alpha transmembrane-coding region (contained in exon 4 of RLA-DR-alpha) and to part of intron 4. These 2 stretches were reversed in DF when compared to other class II genes, and were flanked on both sides by direct repeats of 130 bp with 85% sequence identity. The presence of the transmembrane sequence without the coding sequences that accompany it in DR-alpha, and the observation of the repeats, suggests that the DF region played a role in evolution of class II genes. Genomic blots revealed sequences hybridizing to DF in all rabbits tested and in other lagomorph species. The ratio of replacement to silent substitutions in the DF transmembrane region differed significantly from functional class II genes suggesting an absence of recent selective pressure. An additional direct repeat of the C type, similar to that found in rabbit cytochrome p450, certain class I MHC, TcR-beta and uteroglobin genes, was also present in the DF fragment. C1 NCI,IMMUNOL BRANCH,BETHESDA,MD 20892. NIAID,IMMUNOGENET LAB,ROCKVILLE,MD 20852. RP MARCHE, PN (reprint author), INST PASTEUR,DEPT IMMUNOL,IMMUNOCHIM ANALYT LAB,F-75724 PARIS 15,FRANCE. RI Marche, Patrice/K-5060-2013 OI Marche, Patrice/0000-0002-8930-9340 NR 27 TC 1 Z9 1 U1 0 U2 0 PU EDITIONS SCIENTIFIQUES ELSEVIER PI PARIS CEDEX 15 PA 141 RUE JAVEL, 75747 PARIS CEDEX 15, FRANCE SN 0923-2494 J9 RES IMMUNOL JI Res. Immunol. PD SEP PY 1991 VL 142 IS 7 BP 525 EP 532 DI 10.1016/0923-2494(91)90097-3 PG 8 WC Immunology SC Immunology GA GU256 UT WOS:A1991GU25600002 PM 1812548 ER PT J AU PIZZO, PA AF PIZZO, PA TI THE EVOLVING USE OF BIOLOGICALS IN THE TREATMENT AND PREVENTION OF INFECTIOUS-DISEASES SO REVIEWS OF INFECTIOUS DISEASES LA English DT Editorial Material RP PIZZO, PA (reprint author), NCI,PEDIAT BRANCH,INFECT DIS SECT,BLDG 10,ROOM 13N240,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0162-0886 J9 REV INFECT DIS PD SEP-OCT PY 1991 VL 13 IS 5 BP 971 EP 972 PG 2 WC Immunology; Microbiology SC Immunology; Microbiology GA GJ459 UT WOS:A1991GJ45900031 PM 1962113 ER PT J AU GALLIN, JI AF GALLIN, JI TI INTERFERON-GAMMA IN THE MANAGEMENT OF CHRONIC GRANULOMATOUS-DISEASE SO REVIEWS OF INFECTIOUS DISEASES LA English DT Article ID MACROPHAGE OXIDATIVE-METABOLISM; ANTI-MICROBIAL ACTIVITY; CHROMOSOMAL LOCATION; NADPH OXIDASE; CLONING; GENE; IDENTIFICATION; LYMPHOKINE; CHILDHOOD; COMPONENT AB Bactericidal activity of phagocytic cells depends largely upon their production of highly reactive compounds via the metabolism of oxygen. A lesion anywhere in the biochemical pathway of hydrogen peroxide production potentially can cause chronic granulomatous disease (CGD). Recent work has shown that CGD results from specific abnormalities in the nicotinamide-adenine dinucleotide phosphate (NADPH) system, which includes membrane-associated proteins, NADPH, cytochrome b-558, and several cytosolic proteins. Pharmacologic alteration of phagocytic oxidative metabolism can now be achieved through use of recombinant interferon-gamma (IFN-gamma). Data from a multicenter clinical trial indicate that sustained administration of IFN-gamma is effective in the management of CGD; for patients who received IFN-gamma, a 72% reduction in the relative risk of serious infection was noted in comparison with the risk for patients who received placebo. IFN-gamma reduced not only the number of serious primary infections but also the length of hospitalizations. RP GALLIN, JI (reprint author), NIAID,HOST DEF LAB,BLDG 10,ROOM 11N114,BETHESDA,MD 20892, USA. NR 29 TC 26 Z9 28 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0162-0886 J9 REV INFECT DIS PD SEP-OCT PY 1991 VL 13 IS 5 BP 973 EP 978 PG 6 WC Immunology; Microbiology SC Immunology; Microbiology GA GJ459 UT WOS:A1991GJ45900032 PM 1962114 ER PT J AU MELBY, PC AF MELBY, PC TI EXPERIMENTAL LEISHMANIASIS IN HUMANS - REVIEW SO REVIEWS OF INFECTIOUS DISEASES LA English DT Review ID CUTANEOUS LEISHMANIASIS; MAJOR PROMASTIGOTES; TROPICA VACCINE; IDENTIFICATION; ANTIGENS; INFECTION; SUBSETS; ISRAEL AB Experimental infection of humans with Leishmania parasites has contributed significantly to the understanding of the etiology, transmission, and pathogenesis of leishmaniasis and the immunity associated with it. Leishmania organisms recovered from human and animal tissue, insect vectors, and in vitro cultures have all produced cutaneous or visceral leishmaniasis in human subjects who were voluntarily inoculated with them. Volunteers bitten by infected Phlebotomine sandflies also developed cutaneous or visceral disease. In these experiments, it appeared that the parasite must undergo certain developmental changes within the sandfly for it to become infective and that the parasites in sandflies were far more efficient in causing full-blown infection than were cultured Leishmania organisms. The clinical manifestations of these experimental infections did not differ from infections that were acquired naturally. Natural or experimental infections appeared to confer resistance to subsequent leishmanial infection. This immunity was best documented to be a species-specific phenomenon; however, a small number of studies have demonstrated cross protection between some Leishmania species. In this review article, data from human experimental infections are summarized and discussed in light of recent advances in the field. C1 NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. NR 67 TC 37 Z9 39 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0162-0886 J9 REV INFECT DIS PD SEP-OCT PY 1991 VL 13 IS 5 BP 1009 EP 1017 PG 9 WC Immunology; Microbiology SC Immunology; Microbiology GA GJ459 UT WOS:A1991GJ45900039 PM 1962075 ER PT J AU NUTMAN, TB AF NUTMAN, TB TI EXPERIMENTAL-INFECTION OF HUMANS WITH FILARIAE SO REVIEWS OF INFECTIOUS DISEASES LA English DT Article ID CLINICAL MANIFESTATIONS AB This report summarizes the findings of the 17 published studies involving humans who have been experimentally infected with filarial parasites. Over the past 60 years, 45 individuals have been deliberately infected with Wuchereria bancrofti, Brugia malayi, Brugia pahangi, Loa loa, Mansonella perstans, Mansonella ozzardi, and/or Onchocerca volvulus. The findings from these experimental infections of humans have helped define microfilarial survival and periodicity within human hosts, the prepatent period for the causative agents of lymphatic filariasis, etiologic agents for particular clinical syndromes, immunologic and hematologic consequences of filarial infection, and the role of chemotherapeutic agents in the prevention and treatment of filarial infections. RP NUTMAN, TB (reprint author), NIAID,PARASIT DIS LAB,BLDG 4,ROOM 126,BETHESDA,MD 20892, USA. NR 23 TC 22 Z9 22 U1 1 U2 2 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0162-0886 J9 REV INFECT DIS PD SEP-OCT PY 1991 VL 13 IS 5 BP 1018 EP 1022 PG 5 WC Immunology; Microbiology SC Immunology; Microbiology GA GJ459 UT WOS:A1991GJ45900040 PM 1962076 ER PT J AU PORTIER, CJ KOPPSCHNEIDER, A AF PORTIER, CJ KOPPSCHNEIDER, A TI A MULTISTAGE MODEL OF CARCINOGENESIS INCORPORATING DNA DAMAGE AND REPAIR SO RISK ANALYSIS LA English DT Article DE 2-STAGE MODEL; STOCHASTIC MODEL; BIRTH-DEATH PROCESS; RISK ASSESSMENT ID METHYLENE-CHLORIDE; RISK ASSESSMENT; CANCER; KINETICS; TIME; SKIN C1 DEUTSCH KREBSFORSCHUNGSZENTRUM,INST EPIDEMIOL & BIOMETRIE,BIOSTAT ABT,W-6900 HEIDELBERG,GERMANY. RP PORTIER, CJ (reprint author), NIEHS,DIV BIOMETRY & RISK ASSESSMENT,RES TRIANGLE PK,NC 27709, USA. RI Portier, Christopher/A-3160-2010 OI Portier, Christopher/0000-0002-0954-0279 NR 32 TC 35 Z9 35 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0272-4332 J9 RISK ANAL JI Risk Anal. PD SEP PY 1991 VL 11 IS 3 BP 535 EP 543 DI 10.1111/j.1539-6924.1991.tb00639.x PG 9 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA GJ337 UT WOS:A1991GJ33700024 PM 1947359 ER PT J AU WIENER, LS AF WIENER, LS TI WOMEN AND HUMAN-IMMUNODEFICIENCY-VIRUS - A HISTORICAL AND PERSONAL PSYCHOSOCIAL PERSPECTIVE SO SOCIAL WORK LA English DT Editorial Material RP WIENER, LS (reprint author), NIH,PEDIAT BRANCH,PEDIAT HIV PSYCHOSOCIAL SUPPORT PROGRAM,BETHESDA,MD 20892, USA. NR 13 TC 12 Z9 12 U1 0 U2 1 PU NATL ASSOC SOCIAL WORKERS PI WASHINGTON PA 750 FIRST ST, NE, STE 700, WASHINGTON, DC 20002-4241 SN 0037-8046 J9 SOC WORK JI Soc. Work PD SEP PY 1991 VL 36 IS 5 BP 375 EP 378 PG 4 WC Social Work SC Social Work GA GC924 UT WOS:A1991GC92400002 PM 1843455 ER PT J AU GOFFMAN, TE RAUBITSCHEK, A GLATSTEIN, E AF GOFFMAN, TE RAUBITSCHEK, A GLATSTEIN, E TI SURVIVORS OF HODGKINS-DISEASE - PREVENTION OF SEQUELAE SO SOUTHERN MEDICAL JOURNAL LA English DT Article ID THERAPY; IRRADIATION AB Increasing numbers of patients are surviving Hodgkin's disease in their youth, creating an ever larger cohort who need careful attention to long-term preventive medicine in their older years. Vigilance is especially needed for those who received laparotomy, splenectomy, and irradiation for early stage I through stage III disease, the majority of whom are destined to be long-term survivors. With the frequent migration of the young, these patients often fall under the care of a physician other than their original oncologist. The diligent internist, oncologist, emergency room physician, and general practitioner can avoid several simple pitfalls in the long-term care of these young patients now entering middle age. RP GOFFMAN, TE (reprint author), NCI,DIV CANC TREATMENT,CLIN ONCOL PROGRAM,RADIAT ONCOL BRANCH,B3B69 BLDG 10,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 14 TC 0 Z9 0 U1 0 U2 0 PU SOUTHERN MEDICAL ASSN PI BIRMINGHAM PA 35 LAKESHORE DR PO BOX 190088, BIRMINGHAM, AL 35219 SN 0038-4348 J9 SOUTHERN MED J JI South.Med.J. PD SEP PY 1991 VL 84 IS 9 BP 1108 EP 1110 DI 10.1097/00007611-199109000-00012 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA GG773 UT WOS:A1991GG77300009 PM 1909813 ER PT J AU OLEARY, DH POLAK, JF WOLFSON, SK BOND, MG BOMMER, W SHETH, S PSATY, BM SHARRETT, AR MANOLIO, TA AF OLEARY, DH POLAK, JF WOLFSON, SK BOND, MG BOMMER, W SHETH, S PSATY, BM SHARRETT, AR MANOLIO, TA TI USE OF SONOGRAPHY TO EVALUATE CAROTID ATHEROSCLEROSIS IN THE ELDERLY - THE CARDIOVASCULAR HEALTH STUDY SO STROKE LA English DT Article DE CAROTID ARTERY DISEASES; EPIDEMIOLOGY; ULTRASONICS ID ARTERY ATHEROSCLEROSIS; ANGIOGRAPHY; POPULATION; STENOSIS; RISK; ULTRASONOGRAPHY; PROGRESSION; STROKE AB Carotid sonography is being performed on more than 5,000 participants in the Cardiovascular Health Study, a prospective, multicenter study of cardiovascular disease in men and women aged 65 years and older. The sonographic methods used to examine and measure the extracranial carotid arteries are described. Initial validation studies were performed on 61 subjects with a mean age of 68.6 years. Analysis of within- and between-sonographer differences and between-reader differences were performed for selected variables. In general, the mean absolute differences for within- and between-sonographer comparisons were small, with even less variability between readers. Variability was less for the common carotid artery than for the internal carotid artery. These data suggest that carotid sonography is a reliable and reproducible method for use in the study of carotid atherosclerosis in population studies. C1 BRIGHAM & WOMENS HOSP,DEPT RADIOL,BOSTON,MA 02115. UNIV PITTSBURGH,DEPT NEUROSURG,PITTSBURGH,PA 15260. WAKE FOREST UNIV,BOWMAN GRAY SCH MED,DEPT ANAT,WINSTON SALEM,NC 27103. UNIV CALIF DAVIS,SACRAMENTO MED CTR,DEPT MED,SACRAMENTO,CA 95817. JOHNS HOPKINS UNIV HOSP,DEPT RADIOL,BALTIMORE,MD 21205. UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. UNIV WASHINGTON,DEPT EPIDEMIOL,SEATTLE,WA 98195. UNIV WASHINGTON,DEPT HLTH SERV,SEATTLE,WA 98195. NHLBI,DIV EPIDEMIOL & CLIN APPLICAT,BETHESDA,MD 20892. FU NHLBI NIH HHS [N01-HC85079, N01-HC85080, N01-HC85081] NR 25 TC 435 Z9 441 U1 1 U2 2 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0039-2499 J9 STROKE JI Stroke PD SEP PY 1991 VL 22 IS 9 BP 1155 EP 1163 PG 9 WC Clinical Neurology; Peripheral Vascular Disease SC Neurosciences & Neurology; Cardiovascular System & Cardiology GA GF326 UT WOS:A1991GF32600009 PM 1926258 ER PT J AU GARRISON, CZ LEWINSOHN, PM MARSTELLER, F LANGHINRICHSEN, J LANN, I AF GARRISON, CZ LEWINSOHN, PM MARSTELLER, F LANGHINRICHSEN, J LANN, I TI THE ASSESSMENT OF SUICIDAL-BEHAVIOR IN ADOLESCENTS SO SUICIDE AND LIFE-THREATENING BEHAVIOR LA English DT Article ID IDEATION C1 OREGON RES INST,EUGENE,OR 97403. NIMH,BETHESDA,MD 20892. RP GARRISON, CZ (reprint author), UNIV S CAROLINA,DEPT EPIDEMIOL & BIOSTAT,COLUMBIA,SC 29208, USA. FU PHS HHS [316776, 316774] NR 24 TC 45 Z9 45 U1 0 U2 1 PU GUILFORD PUBLICATIONS INC PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0363-0234 J9 SUICIDE LIFE-THREAT JI Suicide Life-Threat. Behav. PD FAL PY 1991 VL 21 IS 3 BP 217 EP 230 PG 14 WC Psychiatry; Psychology, Multidisciplinary SC Psychiatry; Psychology GA GN473 UT WOS:A1991GN47300001 PM 1759296 ER PT J AU WAALKES, MP DIWAN, BA WEGHORST, CM BARE, RM WARD, JM RICE, JM AF WAALKES, MP DIWAN, BA WEGHORST, CM BARE, RM WARD, JM RICE, JM TI ANTICARCINOGENIC EFFECTS OF CADMIUM IN B6C3F1 MOUSE-LIVER AND LUNG SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID WISTAR CRL-(WI)BR RATS; DOSE-RESPONSE ANALYSIS; IMMUNOHISTOCHEMICAL LOCALIZATION; N-NITROSODIETHYLAMINE; PROMOTING ACTIVITIES; BARBITURIC-ACID; TUMOR-INDUCTION; INJECTION SITE; METALLOTHIONEIN; TOXICITY C1 NCI,DIV CANC ETIOL,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21702. RP WAALKES, MP (reprint author), NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,BIOL CARCINOGENESIS & DEV PROGRAM,BLDG 538,FREDERICK,MD 21702, USA. NR 47 TC 37 Z9 40 U1 0 U2 3 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD SEP 1 PY 1991 VL 110 IS 2 BP 327 EP 335 DI 10.1016/S0041-008X(05)80015-8 PG 9 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA GF537 UT WOS:A1991GF53700015 PM 1891777 ER PT J AU HOEL, DG AF HOEL, DG TI A BALANCED APPROACH TO RISK ASSESSMENT SO TOXICOLOGY AND INDUSTRIAL HEALTH LA English DT Article ID LUNG-CANCER; SUSCEPTIBILITY RP HOEL, DG (reprint author), NIEHS,RES TRIANGLE PK,NC 27709, USA. NR 11 TC 0 Z9 0 U1 0 U2 0 PU PRINCETON SCIENTIFIC PUBL INC PI PRINCETON PA PO BOX 2155, PRINCETON, NJ 08543 SN 0748-2337 J9 TOXICOL IND HEALTH JI Toxicol. Ind. Health PD SEP-NOV PY 1991 VL 7 IS 5-6 BP 305 EP 311 PG 7 WC Public, Environmental & Occupational Health; Toxicology SC Public, Environmental & Occupational Health; Toxicology GA GP113 UT WOS:A1991GP11300034 PM 1780871 ER PT J AU NEMO, GJ MCCURDY, PR AF NEMO, GJ MCCURDY, PR TI PREVENTION OF PLATELET ALLOIMMUNIZATION SO TRANSFUSION LA English DT Editorial Material ID POOR BLOOD COMPONENTS; THROMBOCYTOPENIC PATIENTS; DELAYED ALLOIMMUNIZATION; HLA-ALLOIMMUNIZATION; DENDRITIC CELLS; IMMUNE-RESPONSE; SINGLE DONOR; WHITE CELLS; TRANSFUSIONS; LEUKEMIA C1 NHLBI,DIV BLOOD DIS & RESOURCES,BONE MARROW TRANSPLANTAT BRANCH,BETHESDA,MD 20892. RP NEMO, GJ (reprint author), NHLBI,TRANSFUS MED BRANCH,FED BLDG,ROOM 504,755 WISCONSIN AVE,BETHESDA,MD 20892, USA. NR 32 TC 29 Z9 30 U1 0 U2 1 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 SN 0041-1132 J9 TRANSFUSION JI Transfusion PD SEP PY 1991 VL 31 IS 7 BP 584 EP 586 DI 10.1046/j.1537-2995.1991.31791368332.x PG 3 WC Hematology SC Hematology GA GF911 UT WOS:A1991GF91100003 PM 1891787 ER PT J AU HERBORT, CP DESMET, MD ROBERGE, FG NUSSENBLATT, RB FITZGERALD, D LORBERBOUMGALSKI, H PASTAN, I AF HERBORT, CP DESMET, MD ROBERGE, FG NUSSENBLATT, RB FITZGERALD, D LORBERBOUMGALSKI, H PASTAN, I TI TREATMENT OF CORNEAL ALLOGRAFT-REJECTION WITH THE CYTOTOXIN IL-2-PE40 SO TRANSPLANTATION LA English DT Article ID CYCLOSPORIN-A; GRAFT-REJECTION; LYMPHOCYTES-T; INTERLEUKIN-2; EXPRESSION; RECEPTORS; SURVIVAL; RAT; GROWTH; MODEL AB IL-2-PE40 is a recombinant chimeric protein composed of IL-2, fused to a modified pseudomonas exotoxin. This molecule is extremely toxic to activated T cells expressing high-affinity IL-2R. We used this new molecule for selective immunosuppression to treat corneal allograft rejection in the rat, using Fisher and Lewis rats, a strain combination differing only in medial and minor histocompatibility antigens. The effect of IL-2-PE40 on the immunologic response was studied using both a heterotopic corneal graft model and orthotopic grafts. At the dose of 0.31-mu-g/g given intraperitoneally every 12 hr, IL-2-PE40 produced a significant reduction of both total lymph node cells and cytotoxic-T-cell (CTL) activity in draining lymph nodes (DLN) of heterotopically grafted animals. IL-2-PE40 treatment also significantly reduced the clinical rejection score and cumulative rejection rate (CRR) in orthotopic grafts and appears to be a very effective immunosuppressive agent. C1 NEI,IMMUNOL LAB,BETHESDA,MD 20892. NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. OI de Smet, Marc/0000-0002-9217-5603 NR 22 TC 17 Z9 18 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD SEP PY 1991 VL 52 IS 3 BP 470 EP 474 DI 10.1097/00007890-199109000-00015 PG 5 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA GF766 UT WOS:A1991GF76600015 PM 1897019 ER PT J AU SPIEGEL, AM BUTRYNSKI, JE JONES, TLZ SIMONDS, WF BACKLUND, PS AF SPIEGEL, AM BUTRYNSKI, JE JONES, TLZ SIMONDS, WF BACKLUND, PS TI THE G-PROTEIN CONNECTION - MOLECULAR-BASIS OF MEMBRANE ASSOCIATION SO TRENDS IN BIOCHEMICAL SCIENCES LA English DT Review ID GAMMA SUBUNITS CONTAIN; ALPHA-SUBUNIT; PLASMA-MEMBRANE; BINDING-PROTEIN; GTP-BINDING; BETA-GAMMA; CELLS; MYRISTOYLATION; EXPRESSION; VESICLES AB Two distinct types of lipid modification, myristoylation and isoprenylation, are critical for membrane association of heterotrimeric G proteins. Elucidation of the molecular basis for G protein membrane association has important implications for understanding G protein structure and function, and is relevant to potential therapeutic approaches to AIDS and cancer. C1 NIMH,GEN & COMPARAT BIOCHEM LAB,BETHESDA,MD 20892. RP SPIEGEL, AM (reprint author), NIDDKD,MOLEC PATHOPHYSIOL BRANCH,BETHESDA,MD 20892, USA. NR 30 TC 112 Z9 112 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0968-0004 J9 TRENDS BIOCHEM SCI JI Trends Biochem.Sci. PD SEP PY 1991 VL 16 IS 9 BP 338 EP 341 DI 10.1016/0968-0004(91)90139-M PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GE901 UT WOS:A1991GE90100010 PM 1949155 ER PT J AU HALL, SL MURPHY, BR COELINGH, KLV AF HALL, SL MURPHY, BR COELINGH, KLV TI PROTECTION OF COTTON RATS BY IMMUNIZATION WITH THE HUMAN PARAINFLUENZA VIRUS TYPE-3 FUSION (F) GLYCOPROTEIN EXPRESSED ON THE SURFACE OF INSECT CELLS INFECTED WITH A RECOMBINANT BACULOVIRUS SO VACCINE LA English DT Article DE PIV3 FUSION GLYCOPROTEIN; BACULOVIRUS VECTOR; IMMUNIZATION ID RESPIRATORY SYNCYTIAL VIRUS; PARA-INFLUENZA; HEMAGGLUTININ-NEURAMINIDASE; MONOCLONAL-ANTIBODIES; STRUCTURAL PROTEINS; NUCLEOTIDE-SEQUENCE; VACCINIA VIRUS; HOST IMMUNITY; SENDAI VIRUS; VECTOR AB The antigenicity, immunogenicity and efficacy of the human PIV3 fusion (F) glycoprotein expressed in insect cells by a baculovirus vector were studied. The results indicate that the PIV3 F glycoprotein expressed by a recombinant baculovirus is antigenically authentic as determined using a panel of PIV3 F specific monoclonal antibodies. Only a low level of antibody was stimulated by immunization of animals with infected cells, but the antibody appeared to be of high quality. Immunized animals were also moderately protected against PIV3 challenge. These results indicate that the baculovirus expression system is a reasonable source of authentic PIV3 F protein for use in a subunit vaccine. RP HALL, SL (reprint author), NIAID,INFECT DIS LAB,RESP VIRUSES SECT,BETHESDA,MD 20892, USA. NR 38 TC 18 Z9 18 U1 0 U2 0 PU BUTTERWORTH-HEINEMANN LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0264-410X J9 VACCINE JI Vaccine PD SEP PY 1991 VL 9 IS 9 BP 659 EP 667 DI 10.1016/0264-410X(91)90192-9 PG 9 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA GC529 UT WOS:A1991GC52900010 PM 1659054 ER PT J AU GUPTA, RK AF GUPTA, RK TI DETOXIFICATION OF BORDETELLA-PERTUSSIS ORGANISMS OR PERTUSSIS TOXIN WITH GLUTARALDEHYDE FOR PREPARATION OF PERTUSSIS-VACCINE SO VACCINE LA English DT Letter RP GUPTA, RK (reprint author), NICHHD,DEV & MOLEC IMMUN LAB,BLDG 6,ROOM 1A06,BETHESDA,MD 20892, USA. NR 18 TC 0 Z9 0 U1 0 U2 1 PU BUTTERWORTH-HEINEMANN LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0264-410X J9 VACCINE JI Vaccine PD SEP PY 1991 VL 9 IS 9 BP 690 EP 691 DI 10.1016/0264-410X(91)90205-K PG 2 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA GC529 UT WOS:A1991GC52900015 PM 1950101 ER PT J AU YOSHITOMI, K BOORMAN, GA AF YOSHITOMI, K BOORMAN, GA TI SPONTANEOUS AMELANOTIC MELANOMAS OF THE UVEAL TRACT IN F344 RATS SO VETERINARY PATHOLOGY LA English DT Article DE ALBINO RAT; AMELANOTIC MELANOMAS; UVEAL TRACT AB Five intraocular amelanotic melanomas were identified in the National Toxicology Program's database consisting of records from more than 60,000 female and 60,000 male F344 rats, which were used as control and treated animals in 2-year carcinogenicity studies. The five spontaneous melanomas were grossly observed as white or yellow, unilateral nodules, which originated in the region of the iris and ciliary body, often also involving the choroid. These amelanotic melanomas were composed predominantly of spindle cells arranged in a whorled pattern often with perivascular orientation. Mitotic figures were common in five tumors. The spindle cells had a positive immunoreactivity for S-100 protein but were negative for desmin. Electron microscopic studies provided clear evidence that these tumors originated from the uveal melanocytes. Ultrastructurally, the spindle cells contained numerous cytoplasmic premelanosomes (stage II melanosomes) that were not associated with melanin. Speical histochemical studies showed that the spindle cells had a negative reaction for melanin. Although electron microscopic features are critical in the diagnosis of amelanotic melanomas of the uveal tract, the whorled pattern of spindle cells is a useful histologic criterion in differential diagnosis of this tumor in F344 rats. C1 NIEHS,NATL TOXICOL PROGRAM,CHEM CARCINOGENESIS BRANCH,RES TRIANGLE PK,NC 27709. RP YOSHITOMI, K (reprint author), EXPTL PATHOL LABS INC,POB 12766,RES TRIANGLE PK,NC 27709, USA. NR 22 TC 9 Z9 9 U1 0 U2 0 PU AMER COLL VET PATHOLOGIST PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 SN 0300-9858 J9 VET PATHOL JI Vet. Pathol. PD SEP PY 1991 VL 28 IS 5 BP 403 EP 409 PG 7 WC Pathology; Veterinary Sciences SC Pathology; Veterinary Sciences GA GJ552 UT WOS:A1991GJ55200008 PM 1750166 ER PT J AU OWENS, RA TREMPE, JP CHEJANOVSKY, N CARTER, BJ AF OWENS, RA TREMPE, JP CHEJANOVSKY, N CARTER, BJ TI ADENOASSOCIATED VIRUS REP PROTEINS PRODUCED IN INSECT AND MAMMALIAN EXPRESSION SYSTEMS - WILD-TYPE AND DOMINANT-NEGATIVE MUTANT PROTEINS BIND TO THE VIRAL REPLICATION ORIGIN SO VIROLOGY LA English DT Article ID NUCLEOTIDE-SEQUENCE; DNA-REPLICATION; GENE-EXPRESSION; GENOME ORGANIZATION; SIMIAN VIRUS-40; CELLS; PARVOVIRUS; INVITRO; VECTOR; SITE C1 NIDDKD,MOLEC & CELLULAR BIOL LAB,BLDG 8,ROOM 304,BETHESDA,MD 20892. NR 32 TC 44 Z9 44 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD SEP PY 1991 VL 184 IS 1 BP 14 EP 22 DI 10.1016/0042-6822(91)90817-U PG 9 WC Virology SC Virology GA GA345 UT WOS:A1991GA34500003 PM 1651588 ER PT J AU AGBANDJE, M MCKENNA, R ROSSMANN, MG KAJIGAYA, S YOUNG, NS AF AGBANDJE, M MCKENNA, R ROSSMANN, MG KAJIGAYA, S YOUNG, NS TI PRELIMINARY-X-RAY CRYSTALLOGRAPHIC INVESTIGATION OF HUMAN PARVOVIRUS-B19 SO VIROLOGY LA English DT Article ID B19 HUMAN PARVOVIRUS; APLASTIC CRISIS; INFECTION; GENOME; ANEMIA; VIRUS; PREGNANCY; PARTICLES; OUTBREAK; PROTEINS C1 PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907. NHLBI,CLIN HEMATOL BRANCH,BETHESDA,MD 20892. NR 38 TC 15 Z9 15 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD SEP PY 1991 VL 184 IS 1 BP 170 EP 174 DI 10.1016/0042-6822(91)90833-W PG 5 WC Virology SC Virology GA GA345 UT WOS:A1991GA34500019 PM 1871964 ER PT J AU SMUDA, JW CARTER, BJ AF SMUDA, JW CARTER, BJ TI ADENOASSOCIATED VIRUSES HAVING NONSENSE MUTATIONS IN THE CAPSID GENES - GROWTH IN MAMMALIAN-CELLS CONTAINING AN INDUCIBLE AMBER SUPPRESSOR SO VIROLOGY LA English DT Article ID MURINE LEUKEMIA-VIRUS; VIRAL-DNA REPLICATION; MESSENGER-RNA; NUCLEOTIDE-SEQUENCE; CHLORAMPHENICOL ACETYLTRANSFERASE; STRUCTURAL PROTEINS; ESCHERICHIA-COLI; HUMAN PARVOVIRUS; REP PROTEINS; EXPRESSION C1 NIDDKD,MOLEC & CELLULAR BIOL LAB,BLDG 8,ROOM 304,BETHESDA,MD 20892. NR 46 TC 18 Z9 18 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD SEP PY 1991 VL 184 IS 1 BP 310 EP 318 DI 10.1016/0042-6822(91)90847-5 PG 9 WC Virology SC Virology GA GA345 UT WOS:A1991GA34500033 PM 1651593 ER PT J AU WILLEY, RL KLIMKAIT, T FRUCHT, DM BONIFACINO, JS MARTIN, MA AF WILLEY, RL KLIMKAIT, T FRUCHT, DM BONIFACINO, JS MARTIN, MA TI MUTATIONS WITHIN THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GP160 ENVELOPE GLYCOPROTEIN ALTER ITS INTRACELLULAR-TRANSPORT AND PROCESSING SO VIROLOGY LA English DT Article ID OLIGOMERIC STRUCTURE; ENDOPROTEOLYTIC CLEAVAGE; INFLUENZA HEMAGGLUTININ; HTLV-III/LAV; T-CELLS; PROTEIN; PRECURSOR; SEQUENCE; BINDING; BIOSYNTHESIS C1 NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892. RP WILLEY, RL (reprint author), NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892, USA. RI Klimkait, Thomas/A-1057-2008 NR 38 TC 66 Z9 67 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD SEP PY 1991 VL 184 IS 1 BP 319 EP 329 DI 10.1016/0042-6822(91)90848-6 PG 11 WC Virology SC Virology GA GA345 UT WOS:A1991GA34500034 PM 1871974 ER PT J AU MARTINGALLARDO, A FIEN, KA HU, BT FARLEY, JF SEID, R COLLINS, PL HILDRETH, SW PARADISO, PR AF MARTINGALLARDO, A FIEN, KA HU, BT FARLEY, JF SEID, R COLLINS, PL HILDRETH, SW PARADISO, PR TI EXPRESSION OF THE F-GLYCOPROTEIN GENE FROM HUMAN RESPIRATORY SYNCYTIAL VIRUS IN ESCHERICHIA-COLI - MAPPING OF A FUSION INHIBITING EPITOPE SO VIROLOGY LA English DT Note ID MONOCLONAL-ANTIBODIES; PROTEIN; NEUTRALIZATION; SYSTEM; IDENTIFICATION; INFECTION; IMMUNITY C1 PRAXIS BIOL INC,DEPT MOLEC BIOL,ROCHESTER,NY 14623. PRAXIS BIOL INC,DEPT PROT CHEM,ROCHESTER,NY 14623. NIAID,INFECT DIS LAB,BETHESDA,MD 20892. RP MARTINGALLARDO, A (reprint author), PRAXIS BIOL INC,DEPT VIROL RES,300 E RIVER RD,ROCHESTER,NY 14623, USA. NR 25 TC 21 Z9 23 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD SEP PY 1991 VL 184 IS 1 BP 428 EP 432 DI 10.1016/0042-6822(91)90863-7 PG 5 WC Virology SC Virology GA GA345 UT WOS:A1991GA34500049 PM 1714666 ER PT J AU JIANG, BM QIAN, Y TSUNEMITSU, H GREEN, KY SAIF, LJ AF JIANG, BM QIAN, Y TSUNEMITSU, H GREEN, KY SAIF, LJ TI ANALYSIS OF THE GENE ENCODING THE OUTER CAPSID GLYCOPROTEIN (VP7) OF GROUP-C ROTAVIRUSES BY NORTHERN AND DOT BLOT HYBRIDIZATION SO VIROLOGY LA English DT Note ID PORCINE ROTAVIRUS; SEQUENCE-ANALYSIS; SEROTYPES; NEUTRALIZATION; PROBES; DIFFERENTIATION; POLYPEPTIDES; OUTBREAK; PROTEIN C1 OHIO STATE UNIV,OHIO AGR RES & DEV CTR,FOOD ANIM HLTH RES PROGRAM,WOOSTER,OH 44691. NIH,INFECT DIS LAB,BETHESDA,MD 20892. NR 25 TC 19 Z9 20 U1 1 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD SEP PY 1991 VL 184 IS 1 BP 433 EP 436 DI 10.1016/0042-6822(91)90864-8 PG 4 WC Virology SC Virology GA GA345 UT WOS:A1991GA34500050 PM 1651601 ER PT J AU LJUNGQVIST, A FENG, DM BOWERS, C HOOK, WA FOLKERS, K AF LJUNGQVIST, A FENG, DM BOWERS, C HOOK, WA FOLKERS, K TI DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF ANTAGONISTS OF LHRH BY CRITERIA OF POTENCY, SAFETY AND SOLUBILITY SO ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES LA English DT Article DE LHRH-ANTAGONISTS; ANTIOVULATORY ACTIVITY; HISTAMINE RELEASE; SOLUBILITY ID HORMONE-RELEASING HORMONE; LYS GNRH ANTAGONIST; HISTAMINE-RELEASE; GONADOTROPIN INHIBITION; PROLONGED DURATION; ANTIDE; ANALOGS; MONKEYS; RATS AB Some analogs of Antide and congeners with higher water solubility have been synthesized by substitutions in positions 1, 5 or 6 with hydrophilic residues. In position 1, D-3-Qal has been incorporated in four peptides and D-3-Pal in one peptide. In positions 5 and 6, D and L-3-Pal, PzAla and (DSer)Lys have been tried. In one peptide, D-(AcDSer)Lys was substituted in position 6. Most of the new analogs had lower AOA (antiovulatory activity) than the parent compounds but three potent analogs were identified. The first one, [N-Ac-D-3-Qal1,DpClPhe2,D-3-Pal3,c-PzACAla5,D-PicLys6,ILys8,D-Ala10]-LHRH, had 55% AOA at 0.25-mu-g and 100% at 0.5-mu-g. Its ED50 for in vitro histamine release was 171 +/- 17-mu-g/ml which is an increase from 49 +/- 4.8-mu-g/ml for the parent compound with N-Ac-D-2-Nal[1]. The second analog, [N-Ac-D-2-Nal1,DpClPhe2,D-3-Pal3,PicLys5,D-(DSer)Lys6,ILys8,D-Ala10]-LHRH, had 69% AOA at 0.25-mu-g and 95% at 0.5-mu-g. This analog released somewhat more histamine than the parent analog featuring D-PicLys6, the ED50 being 18-mu-g/ml compared to 93 +/- 11 for the parent analog. The third analog is: [N-Ac-D-2-Nal1,DpClPhe2,D-3-Pal3,c-PzACAla5,D-PzAla6,ILys8,DAla1]-LHRH. The AOA for this analog was 63% at 0.25-mu-g and the ED50 for histamine release 88 +/- 6.4-mu-g/ml. C1 UNIV TEXAS,INST BIOMED RES,AUSTIN,TX 78712. TULANE UNIV,SCH MED,NEW ORLEANS,LA 70112. NIDR,BETHESDA,MD 20892. NR 26 TC 7 Z9 7 U1 0 U2 0 PU VERLAG Z NATURFORSCH PI TUBINGEN PA POSTFACH 2645, W-7400 TUBINGEN, GERMANY SN 0932-0776 J9 Z NATURFORSCH B JI Z.Naturforsch.(B) PD SEP PY 1991 VL 46 IS 9 BP 1231 EP 1236 PG 6 WC Chemistry, Inorganic & Nuclear; Chemistry, Organic SC Chemistry GA GH252 UT WOS:A1991GH25200017 ER PT J AU CHABNER, BA ROTHENBERG, ML AF CHABNER, BA ROTHENBERG, ML TI MEDICAL ONCOLOGY IN THE 1990S SO LANCET LA English DT Letter ID BONE-MARROW TRANSPLANTATION; COLORECTAL-CARCINOMA; LEUKEMIA; FLUOROURACIL; MODULATION; PHASE RP CHABNER, BA (reprint author), NCI,DIV CANC TREATMENT,BETHESDA,MD 20892, USA. NR 10 TC 1 Z9 1 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD AUG 31 PY 1991 VL 338 IS 8766 BP 576 EP 577 DI 10.1016/0140-6736(91)91146-L PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA GD002 UT WOS:A1991GD00200044 PM 1678834 ER PT J AU BABOONIAN, C DALGLEISH, A BOUNTIFF, L GROSS, J OROSZLAN, S RICKETT, G SMITHBURCHNELL, C TROKE, P MERSON, J AF BABOONIAN, C DALGLEISH, A BOUNTIFF, L GROSS, J OROSZLAN, S RICKETT, G SMITHBURCHNELL, C TROKE, P MERSON, J TI HIV-1 PROTEINASE IS REQUIRED FOR SYNTHESIS OF PRO-VIRAL DNA SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; ESCHERICHIA-COLI; PROTEASE; INFECTIVITY; GENE; EXPRESSION C1 PFIZER LTD,PFIZER CENT RES,SANDWICH CT13 9NJ,KENT,ENGLAND. CLIN RES CTR,HARROW HA1 3UJ,MIDDX,ENGLAND. NCI,FREDERICK CANC RES FACIL,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21701. NR 19 TC 48 Z9 48 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD AUG 30 PY 1991 VL 179 IS 1 BP 17 EP 24 DI 10.1016/0006-291X(91)91327-9 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GD092 UT WOS:A1991GD09200003 PM 1652947 ER PT J AU TAHARA, K BAN, T MINEGISHI, T KOHN, LD AF TAHARA, K BAN, T MINEGISHI, T KOHN, LD TI IMMUNOGLOBULINS FROM GRAVES-DISEASE PATIENTS INTERACT WITH DIFFERENT SITES ON TSH RECEPTOR LH-CG RECEPTOR CHIMERAS THAN EITHER TSH OR IMMUNOGLOBULINS FROM IDIOPATHIC MYXEDEMA PATIENTS SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID HUMAN THYROTROPIN RECEPTOR; CHROMOSOMAL ASSIGNMENT; MONOCLONAL-ANTIBODIES; EXPRESSION; AUTOANTIBODIES; CLONING; SEQUENCE; INCREASE; FAMILY; CDNA C1 GUNMA UNIV,SCH MED,DEPT OBSTET & GYNECOL,MAEBASHI,GUNMA 371,JAPAN. RP TAHARA, K (reprint author), NIDDKD,BIOCHEM & METAB LAB,CELL REGULAT SECT,BETHESDA,MD 20892, USA. NR 21 TC 127 Z9 130 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD AUG 30 PY 1991 VL 179 IS 1 BP 70 EP 77 DI 10.1016/0006-291X(91)91335-A PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GD092 UT WOS:A1991GD09200011 PM 1883391 ER PT J AU TAKAI, O DESAI, RK SEETHARAMAIAH, GS JONES, CA ALLAWAY, GP AKAMIZU, T KOHN, LD PRABHAKAR, BS AF TAKAI, O DESAI, RK SEETHARAMAIAH, GS JONES, CA ALLAWAY, GP AKAMIZU, T KOHN, LD PRABHAKAR, BS TI PROKARYOTIC EXPRESSION OF THE THYROTROPIN RECEPTOR AND IDENTIFICATION OF AN IMMUNOGENIC REGION OF THE PROTEIN USING SYNTHETIC PEPTIDES SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID TSH RECEPTOR; MOLECULAR-CLONING; BINDING; AUTOANTIBODIES; LOCALIZATION; DISEASE; SITE C1 NIDDK,BIOCHEM & METAB LAB,CELL REGULAT SECT,BETHESDA,MD 20892. NIDR,ORAL MED LAB,BETHESDA,MD 20892. RP TAKAI, O (reprint author), UNIV TEXAS,MED BRANCH,DEPT MICROBIOL,GALVESTON,TX 77550, USA. NR 19 TC 67 Z9 67 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD AUG 30 PY 1991 VL 179 IS 1 BP 319 EP 326 DI 10.1016/0006-291X(91)91372-J PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GD092 UT WOS:A1991GD09200048 PM 1883361 ER PT J AU SOHN, DH YUN, YP PARK, KS VEECH, RL SONG, BJ AF SOHN, DH YUN, YP PARK, KS VEECH, RL SONG, BJ TI POSTTRANSLATIONAL REDUCTION OF CYTOCHROME P450IIE BY CCL4, ITS SUBSTRATE SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID CARBON-TETRACHLORIDE; RAT; INDUCTION; ACID; STABILIZATION; ACETONE; INVITRO; INVIVO; SINGLE; FORM RP SOHN, DH (reprint author), NIAAA,METAB & MOLEC BIOL LAB,ROCKVILLE,MD 20852, USA. NR 21 TC 42 Z9 43 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD AUG 30 PY 1991 VL 179 IS 1 BP 449 EP 454 DI 10.1016/0006-291X(91)91391-O PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GD092 UT WOS:A1991GD09200067 PM 1883371 ER PT J AU GUSZCZYNSKI, T CHRAMBACH, A AF GUSZCZYNSKI, T CHRAMBACH, A TI ELECTROPHORETIC SEPARATION OF S-POMBE CHROMOSOMES IN POLYACRYLAMIDE SOLUTIONS USING A CONSTANT FIELD SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID GEL-ELECTROPHORESIS RP GUSZCZYNSKI, T (reprint author), NICHHD,THEORET & PHYS BIOL LAB,MACROMOLEC ANAL SECT,BETHESDA,MD 20892, USA. NR 10 TC 21 Z9 21 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD AUG 30 PY 1991 VL 179 IS 1 BP 482 EP 486 DI 10.1016/0006-291X(91)91396-T PG 5 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GD092 UT WOS:A1991GD09200072 PM 1883373 ER PT J AU PARKISON, C ASHIZAWA, K MCPHIE, P LIN, KH CHENG, SY AF PARKISON, C ASHIZAWA, K MCPHIE, P LIN, KH CHENG, SY TI THE MONOMER OF PYRUVATE-KINASE, SUBTYPE-M1, IS BOTH A KINASE AND A CYTOSOLIC THYROID-HORMONE BINDING-PROTEIN SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID RAT-KIDNEY; ISOZYMES C1 NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. NIDDKD,BIOCHEM & METAB LAB,BETHESDA,MD 20892. NR 13 TC 18 Z9 18 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD AUG 30 PY 1991 VL 179 IS 1 BP 668 EP 674 DI 10.1016/0006-291X(91)91424-B PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GD092 UT WOS:A1991GD09200100 PM 1883388 ER PT J AU MENKEL, M TERRY, P PONTECORVO, M KATZ, JL WITKIN, JM AF MENKEL, M TERRY, P PONTECORVO, M KATZ, JL WITKIN, JM TI SELECTIVE SIGMA-LIGANDS BLOCK STIMULANT EFFECTS OF COCAINE SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Note ID RECEPTORS C1 NIDA,ADDICT RES CTR,PSYCHOBIOL LAB,DRUG DEV GRP,POB 5180,BALTIMORE,MD 21224. NR 5 TC 93 Z9 94 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD AUG 29 PY 1991 VL 201 IS 2-3 BP 251 EP 252 DI 10.1016/0014-2999(91)90355-T PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GE944 UT WOS:A1991GE94400021 PM 1686761 ER PT J AU DOLCI, S WILLIAMS, DE ERNST, MK RESNICK, JL BRANNAN, CI LOCK, LF LYMAN, SD BOSWELL, HS DONOVAN, PJ AF DOLCI, S WILLIAMS, DE ERNST, MK RESNICK, JL BRANNAN, CI LOCK, LF LYMAN, SD BOSWELL, HS DONOVAN, PJ TI REQUIREMENT FOR MAST-CELL GROWTH-FACTOR FOR PRIMORDIAL GERM-CELL SURVIVAL IN CULTURE SO NATURE LA English DT Article ID RECEPTOR TYROSINE KINASES; C-KIT; SI-LOCUS; LIGAND; MOUSE; IDENTIFICATION; PROGENITORS; EXPRESSION; CLONING; RAT AB MAST-CELL growth factor (MGF) is encoded by the murine steel (Sl) locus and is a ligand for the tyrosine kinase receptor protein encoded by the proto-oncogene c-kit at the murine dominant white spotting (W) locus. Mutations at both these loci affect mast cells, primordial germ cells (PGCs), haemopoietic stem cells and melanocytes. In many Sl and W mutants, the rapid proliferation of PGC that normally occurs between day 7 and 13.5 of embryonic development fails to occur. As c-kit is expressed in PGCs 1,2 while MGF is expressed in the surrounding mesenchyme 2,3, MGF might promote the proliferation of PGCs. Here we report that MGF is essential for PGC survival in culture, but does not stimulate PGC proliferation. Moreover, whereas both the transmembrane and soluble proteolytic cleavage forms of MGF stimulate mast-cell proliferation, soluble MGF has a relatively limited ability to support survival of PGCs in culture, thus explaining the sterility in mice carrying the steel-dickie (Sl(d)) mutation, which encodes only a soluble form of MGF, and providing a functional role for a transmembrane growth factor. C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MOLEC MECHANISMS CARCINOGENESIS LAB,FREDERICK,MD 21702. IMMUNEX CORP,DEPT EXPTL HEMATOL,SEATTLE,WA 98101. IMMUNEX CORP,DEPT MOLEC BIOL,SEATTLE,WA 98101. INDIANA UNIV,SCH MED,INDIANAPOLIS,IN 46202. UNIV ROME TOR VERGATA 2,DIPARTIMENTO SANITA PUBBL & BIOL CELLULARE,I-00173 ROME,ITALY. RI dolci, susanna/B-8363-2013; OI dolci, susanna/0000-0002-6864-3673 NR 26 TC 401 Z9 405 U1 0 U2 5 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD AUG 29 PY 1991 VL 352 IS 6338 BP 809 EP 811 DI 10.1038/352809a0 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GC964 UT WOS:A1991GC96400062 PM 1715518 ER PT J AU POTTER, WZ RUDORFER, MV MANJI, H AF POTTER, WZ RUDORFER, MV MANJI, H TI DRUG-THERAPY - THE PHARMACOLOGICAL TREATMENT OF DEPRESSION SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Review ID DEXAMETHASONE SUPPRESSION TEST; BETA-ADRENERGIC RECEPTORS; OBSESSIVE-COMPULSIVE DISORDER; MONOAMINE-OXIDASE INHIBITORS; RESEARCH DIAGNOSTIC-CRITERIA; PLATELET ADENYLATE-CYCLASE; ENDOGENOUS-DEPRESSION; UNIPOLAR DEPRESSION; TRICYCLIC ANTIDEPRESSANTS; ELECTROCONVULSIVE-THERAPY RP POTTER, WZ (reprint author), NIMH,EXPTL THERAPEUT BRANCH,CLIN PHARMACOL SECT,BLDG 10,RM 2D46,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 138 TC 191 Z9 192 U1 2 U2 3 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD AUG 29 PY 1991 VL 325 IS 9 BP 633 EP 642 PG 10 WC Medicine, General & Internal SC General & Internal Medicine GA GC328 UT WOS:A1991GC32800007 PM 1861697 ER PT J AU PIZZO, PA BUTLER, KM AF PIZZO, PA BUTLER, KM TI IN THE VERTICAL TRANSMISSION OF HIV, TIMING MAY BE EVERYTHING SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Editorial Material ID HUMAN IMMUNODEFICIENCY VIRUS RP PIZZO, PA (reprint author), NCI,BETHESDA,MD 20892, USA. NR 13 TC 58 Z9 58 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD AUG 29 PY 1991 VL 325 IS 9 BP 652 EP 654 DI 10.1056/NEJM199108293250909 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA GC328 UT WOS:A1991GC32800009 PM 1861699 ER PT J AU HALLETT, M AF HALLETT, M TI CLASSIFICATION AND TREATMENT OF TREMOR SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Discussion ID PRIMARY WRITING TREMOR; PARKINSONS-DISEASE; MULTIPLE-SCLEROSIS; PRIMIDONE RP HALLETT, M (reprint author), NINCDS,MED NEUROL BRANCH,HUMAN MOTOR CONTROL SECT,BLDG 10,ROOM 5N-226,BETHESDA,MD 20892, USA. NR 25 TC 51 Z9 51 U1 0 U2 2 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD AUG 28 PY 1991 VL 266 IS 8 BP 1115 EP 1117 DI 10.1001/jama.266.8.1115 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA GB975 UT WOS:A1991GB97500036 PM 1650852 ER PT J AU BASTA, M HAMMER, CH AF BASTA, M HAMMER, CH TI A RAPID FPLC METHOD FOR PURIFICATION OF THE 3RD COMPONENT OF HUMAN AND GUINEA-PIG COMPLEMENT SO JOURNAL OF IMMUNOLOGICAL METHODS LA English DT Article DE CHROMATOGRAPHY, FAST PROTEIN LIQUID; CHROMATOGRAPHY, ANION-EXCHANGE; C3, HUMAN; C3, GUINEA PIG; PURIFICATION METHOD AB A method is described for the purification of human and guinea pig C3 from small amounts of serum. This procedure requires only two steps - polyethylene glycol (PEG) precipitation and fast protein liquid chromatography (FPLC) Mono Q HR 10/10 ion exchange chromatography. The protocol takes less than two hours to complete and yields 4-6 mg of purified C3. Similar results, in terms of antigenic and functional recovery, were obtained for both human and guinea pig components. About 67% of C3 antigen was recovered from eluted fractions with fully preserved specific activity. Isolated C3 was over 95% pure as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography; this level of purity was confirmed by the absence of any observable contamination as assessed by immunoelectrophoresis using high titer anti-whole human serum. This method allows rapid and reproducible purification of fully active human or guinea pig C3 on a daily basis. RP BASTA, M (reprint author), NIAID,CLIN INVEST LAB,BLDG 10,ROOM 11N228,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. OI Basta, Milan/0000-0001-5958-9241 NR 9 TC 19 Z9 19 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1759 J9 J IMMUNOL METHODS JI J. Immunol. Methods PD AUG 28 PY 1991 VL 142 IS 1 BP 39 EP 44 DI 10.1016/0022-1759(91)90290-V PG 6 WC Biochemical Research Methods; Immunology SC Biochemistry & Molecular Biology; Immunology GA GD761 UT WOS:A1991GD76100005 PM 1919020 ER PT J AU VINCENT, JS REVAK, SD COCHRANE, CG LEVIN, IW AF VINCENT, JS REVAK, SD COCHRANE, CG LEVIN, IW TI RAMAN-SPECTROSCOPIC STUDIES OF MODEL HUMAN PULMONARY SURFACTANT SYSTEMS - PHOSPHOLIPID INTERACTIONS WITH PEPTIDE PARADIGMS FOR THE SURFACTANT PROTEIN SP-B SO BIOCHEMISTRY LA English DT Article ID AMINO-ACID SEQUENCES; LUNG SURFACTANT; MEMBRANE BILAYERS; ACTIVE MATERIAL; APOPROTEINS; CDNA; RECONSTITUTION; TRANSITIONS AB The temperature dependence of dipalmitoylphosphatidylcholine (DPPC)/phosphatidylglycerol (PG) multilayers, reconstituted with various synthetic peptides for modeling human lung surfactant, was monitored by vibrational Raman spectroscopy. The synthetic peptides consisted, respectively, of residues 59-81 of the human surfactant protein SP-B and 21 amino acid residue peptides containing repeating units of arginine separated by either four or eight leucines (RL4 or RL8). Each peptide demonstrated the ability to reduce significantly the surface tension of analogues of the phospholipid mixture used in the Raman studies. Raman spectroscopic integrated band intensities and relative peak height intensity ratios, two spectral parameters used to determine bilayer disorder, provided sensitive probes for characterizing multilayer perturbations in the reconstituted liposomes. Temperature profiles derived from the various Raman intensity parameters for the 3100-2800-cm-1 carbon-hydrogen (C-H) stretching mode region, a spectral interval representative of acyl chain vibrations, reflected lipid reorganizations due to the bilayer interactions of these peptides. For the three reconstituted multilamellar surfactant systems, the gel-to-liquid-crystalline phase-transition temperatures T(m), defined by acyl chain C-H stretching mode order/disorder parameters, increased from 35-degrees-C in the peptide free system to 37-38-degrees-C, indicating increased lipid headgroup constraints for the model liposomes. Although the values of T(m) were similar for the three recombinant lipid/peptide assemblies, individual phase-transition cooperativities varied significantly between systems and between spectroscopically derived order/disorder parameters. C1 UNIV MARYLAND, DEPT CHEM, 5401 WILKENS AVE, CATONSVILLE, MD 21228 USA. NIDDKD, CHEM PHYS LAB, BETHESDA, MD 20892 USA. Scripps Res Inst, RES INST, DEPT IMMUNOL, LA JOLLA, CA 92037 USA. FU NHLBI NIH HHS [HL 23584] NR 31 TC 40 Z9 40 U1 3 U2 13 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD AUG 27 PY 1991 VL 30 IS 34 BP 8395 EP 8401 DI 10.1021/bi00098a017 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GC959 UT WOS:A1991GC95900017 PM 1883825 ER PT J AU HAYES, JJ BASHKIN, J TULLIUS, TD WOLFFE, AP AF HAYES, JJ BASHKIN, J TULLIUS, TD WOLFFE, AP TI THE HISTONE CORE EXERTS A DOMINANT CONSTRAINT ON THE STRUCTURE OF DNA IN A NUCLEOSOME SO BIOCHEMISTRY LA English DT Article ID B-DNA; HELICAL PERIODICITY; KINETOPLAST DNA; MINOR GROOVE; SEQUENCE; CHROMATIN; POLY(DA).POLY(DT); PROTEIN; CONFORMATION; TRACTS AB We have examined the structures of unique sequence, A/T-rich DNAs that are predicted to be relatively rigid [oligo(dA).oligo(dT)], flexible [oligo[d(A-T)]], and curved, using the hydroxyl radical as a cleavage reagent. A 50-base-pair segment containing each of these distinct DNA sequences was placed adjacent to the T7 RNA polymerase promoter, a sequence that will strongly position nucleosomes. The final length of the DNA fragments was 142 bp, enough DNA to assemble a single nucleosome. Cleavage of DNA in solution, while bound to a calcium phosphate crystal, and after incorporation into a nucleosome is examined. We find that the distinct A/T-rich DNAs have very different structural features in solution and helical periodicities when bound to calcium phosphate. In contrast, the organization of the different DNA sequences when associated with a histone octamer is very similar. We conclude that the histone core exerts a dominant constraint on the structure of DNA in a nucleosome and that inclusion of these various unique sequences has only a very small effect on overall nucleosome stability and structure. C1 NICHHD,MOLEC EMBRYOL LAB,BLDG 6,ROOM 131,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,DEPT CHEM,BALTIMORE,MD 21218. RI Tullius, Thomas/A-9685-2008 OI Tullius, Thomas/0000-0003-4425-796X FU NCI NIH HHS [CA 01208]; NIGMS NIH HHS [GM 41930] NR 50 TC 68 Z9 68 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD AUG 27 PY 1991 VL 30 IS 34 BP 8434 EP 8440 DI 10.1021/bi00098a022 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GC959 UT WOS:A1991GC95900022 PM 1653013 ER PT J AU TOMINAGA, S JENKINS, NA GILBERT, DJ COPELAND, NG TETSUKA, T AF TOMINAGA, S JENKINS, NA GILBERT, DJ COPELAND, NG TETSUKA, T TI MOLECULAR-CLONING OF THE MURINE ST2 GENE - CHARACTERIZATION AND CHROMOSOMAL MAPPING SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Article DE CELL GROWTH; CELL CYCLE; INTERLEUKIN-1 RECEPTOR; IMMUNOGLOBULIN SUPERFAMILY ID INTERLEUKIN-1 RECEPTOR ANTAGONIST; BETA-SUBUNIT; IMMUNOGLOBULIN SUPERFAMILY; PROMOTER REGION; DNA-SEQUENCES; G1 PHASE; G0 STATE; EXPRESSION; CELLS; TRANSCRIPTION AB The genomic locus of the murine ST2 gene was isolated based on homology with a murine ST2 complementary DNA sequence and its complete nucleotide sequence was determined. The locus is composed of eight exons and seven introns and is approx. 9 kilobase pairs in size. Two Sp1 binding sites are present in the 5' flanking region. The murine ST2 gene, which was expressed only in the growth-stimulated BALB/c-3T3 cells, was mapped to mouse chromosome one, very tightly linked to the interleukin 1 receptor-type 1 locus. C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21701. RP TOMINAGA, S (reprint author), JICHI MED SCH,DEPT BIOCHEM 2,3311-1 YAKUSHIJI,MINAMI KAWACHI,TOCHIGI 32904,JAPAN. FU NCI NIH HHS [N01-CO-74101] NR 33 TC 68 Z9 69 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD AUG 27 PY 1991 VL 1090 IS 1 BP 1 EP 8 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GE947 UT WOS:A1991GE94700001 PM 1832015 ER PT J AU STEWART, WW AF STEWART, WW TI THE BALTIMORE FIASCO - A CASE-STUDY IN FRAUD SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 5 EP PROF PN 2 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG081 UT WOS:A1991HG08101457 ER PT J AU LUNN, G SANSONE, EB AF LUNN, G SANSONE, EB TI DECONTAMINATION OF SOLUTIONS OF HAZARDOUS COMPOUNDS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,ENVIRONM CONTROL & RES PROGRAM,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 6 EP CHAS PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08001264 ER PT J AU IRWIN, PL CHAMULITRAT, W SEVILLA, MD HOFFMAN, AE AF IRWIN, PL CHAMULITRAT, W SEVILLA, MD HOFFMAN, AE TI NITROXYL AMIDE SPIN LABELING - METHYL ESTERIFICATION-INDUCED AND HYDRATION-INDUCED MOTIONAL PERTURBATIONS OF PECTINIC POLYSACCHARIDES IN APPLE TISSUE SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 USDA ARS,EASTERN REG RES CTR,PHILADELPHIA,PA 19118. NIH,MOLEC BIOPHYS LAB,BETHESDA,MD 20892. OAKLAND UNIV,DEPT CHEM,ROCHESTER,MI 48063. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 12 EP AGFD PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08000014 ER PT J AU BLUMBERG, PM AF BLUMBERG, PM TI NATURAL-PRODUCTS AS PROBES OF PROTEIN-KINASE-C HETEROGENEITY SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 17 EP MEDI PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08002542 ER PT J AU GONZALEZ, FJ AF GONZALEZ, FJ TI HUMAN CYTOCHROMES P450 AND THEIR ROLES IN ENVIRONMENTALLY BASED DISEASE SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 21 EP ACSC PN 2 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG081 UT WOS:A1991HG08101506 ER PT J AU BURKE, TR LIM, B ESCOBEDO, J WILLIAMS, LT MARQUEZ, VE AF BURKE, TR LIM, B ESCOBEDO, J WILLIAMS, LT MARQUEZ, VE TI CARBOXYNAPHTHYL, CARBOXYQUINOLYL AND CARBOXYISOQUINOLYL COMPOUNDS AS BICYCLIC RING-CONSTRAINED ANALOGS OF STYRYL-BASED PROTEIN TYROSINE KINASE INHIBITORS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI,DEPT MED CHEM,BETHESDA,MD 20892. UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 23 EP MEDI PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08002488 ER PT J AU MCMURRY, TJ BRECHBIEL, M KUMAR, K GANSOW, O AF MCMURRY, TJ BRECHBIEL, M KUMAR, K GANSOW, O TI SYNTHESIS OF MACROCYCLIC BIFUNCTIONAL CHELATING-AGENTS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIH,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 24 EP ORGN PN 2 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG081 UT WOS:A1991HG08100117 ER PT J AU NICKLAUS, MC MILNE, GWA BURKE, TR AF NICKLAUS, MC MILNE, GWA BURKE, TR TI COMPARATIVE MOLECULAR-FIELD ANALYSIS OF CONFORMATIONALLY FLEXIBLE TYROSINE KINASE INHIBITORS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI,DEPT MED CHEM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 24 EP MEDI PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08002489 ER PT J AU PIPPIN, CG BRECHBIEL, MW MCMURRY, TJ LIU, H GANSOW, OA AF PIPPIN, CG BRECHBIEL, MW MCMURRY, TJ LIU, H GANSOW, OA TI COORDINATION OF BISMUTH(III) TO POLYAMINOCARBOXYLATE LIGANDS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 33 EP INOR PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08002086 ER PT J AU LIM, BB MARQUEZ, VE DOBYNS, KA COONEY, DA DECLERCQ, E AF LIM, BB MARQUEZ, VE DOBYNS, KA COONEY, DA DECLERCQ, E TI SYNTHESIS AND BIOLOGICAL STUDY OF THE CYCLOPENTENYL CARBOCYCLIC NUCLEOSIDE ANALOG OF 5-AZACYTIDINE SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI,MED CHEM LAB,BETHESDA,MD 20892. CATHOLIC UNIV LEUVEN,REGA INST MED RES,B-3000 LOUVAIN,BELGIUM. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 48 EP MEDI PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08002513 ER PT J AU HUANG, LQ MOORE, C MCGOWN, S TOMER, KB TONG, HY AF HUANG, LQ MOORE, C MCGOWN, S TOMER, KB TONG, HY TI THE APPLICATION OF HIGH-RESOLUTION MS AND HYBRID MS MS TO THE ANALYSIS OF FLY-ASH FOR MONOBROMINATED POLYCHLORINATED DIBENZO-PARA-DIOXINS AND DIBENZOFURANS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 CONNECTICUT AGR EXPT STN,DEPT ANALYT CHEM,NEW HAVEN,CT 06611. KRATOS ANALYT INC,RAMSEY,NJ 07446. NIEHS,RES TRIANGLE PK,NC 27709. UNIV NEBRASKA,DEPT CHEM,MCMS,LINCOLN,NE 68588. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 50 EP ANYL PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08000419 ER PT J AU MCDIARMID, R AF MCDIARMID, R TI DIENE EXCITED-STATE ENERGETICS, STRUCTURES, AND DYNAMICS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 75 EP PHYS PN 2 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG081 UT WOS:A1991HG08100611 ER PT J AU BAX, A DELAGLIO, F IKURA, M KAY, LE CLORE, M GRONENBORN, A TORCHIA, D AF BAX, A DELAGLIO, F IKURA, M KAY, LE CLORE, M GRONENBORN, A TORCHIA, D TI MULTIDIMENSIONAL NMR OF ISOTOPICALLY ENRICHED PROTEINS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIADDKD,CHEM PHYS LAB,BETHESDA,MD 20892. NIDR,BONE RES BRANCH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 113 EP PHYS PN 2 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG081 UT WOS:A1991HG08100649 ER PT J AU VANGALEN, PJM MICHAELS, GS IJZERMAN, AP STILES, GL JACOBSON, KA AF VANGALEN, PJM MICHAELS, GS IJZERMAN, AP STILES, GL JACOBSON, KA TI SEQUENCE-ANALYSIS AND COMPUTER MODELING OF THE DOG A1 AND A2 ADENOSINE RECEPTORS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. LEIDEN UNIV,CBPS,2300 RA LEIDEN,NETHERLANDS. DUKE UNIV,MED CTR,DURHAM,NC 27710. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 114 EP MEDI PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08002579 ER PT J AU MAILLARD, MC NIKODIJEVIC, O BAUMGOLD, J EVELETH, D LANOUE, KF JI, XD BARTUS, RT JACOBSON, KA AF MAILLARD, MC NIKODIJEVIC, O BAUMGOLD, J EVELETH, D LANOUE, KF JI, XD BARTUS, RT JACOBSON, KA TI ADENOSINE RECEPTOR PRODRUGS - SYNTHESIS AND BIOLOGICAL-ACTIVITY OF DERIVATIVES OF POTENT A1-SELECTIVE AGONISTS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. GEORGE WASHINGTON UNIV,WASHINGTON,DC 20052. CORTEX PHARMACEUT INC,IRVINE,CA. PENN STATE UNIV,MILTON S HERSHEY MED CTR,HERSHEY,PA 17033. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 115 EP MEDI PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08002580 ER PT J AU MOSELEY, MA TOMER, KB DETERDING, LJ JORGENSON, JW AF MOSELEY, MA TOMER, KB DETERDING, LJ JORGENSON, JW TI APPLICATION OF CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY AS A DETECTOR FOR LIQUID-PHASE SEPARATION SYSTEMS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 GLAXO RES INST,DEPT DRUG METAB,RES TRIANGLE PK,NC 27709. NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709. UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27514. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 125 EP ANYL PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08000493 ER PT J AU LEVY, LA RAJU, B MURPHY, E LONDON, RE AF LEVY, LA RAJU, B MURPHY, E LONDON, RE TI DEVELOPMENT OF NMR ACTIVE INDICATORS FOR MEASUREMENT OF CALCIUM-ION CONCENTRATION SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 149 EP ORGN PN 2 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG081 UT WOS:A1991HG08100242 ER PT J AU CAPSON, TL BENKOVIC, SJ NOSSAL, NG AF CAPSON, TL BENKOVIC, SJ NOSSAL, NG TI KINETIC AND STRUCTURAL STUDIES OF THE T4 DNA-REPLICATION SYSTEM SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 PENN STATE UNIV,DAVEY LAB 152,UNIV PK,PA 16802. NIDDK,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 159 EP BIOL PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08000916 ER PT J AU NASR, M CRADOCK, J JOHNSTON, M AF NASR, M CRADOCK, J JOHNSTON, M TI STRUCTURE-ACTIVITY CORRELATIONS OF NATURAL-PRODUCTS WITH ANTI-HIV ACTIVITY SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIAID,DIV AIDS,ROCKVILLE,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 163 EP AGFD PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08000165 ER PT J AU LEDNICER, D NARAYANAN, VL AF LEDNICER, D NARAYANAN, VL TI NATURAL-PRODUCTS AS ANTI-HIV AGENTS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI,DRUG SYNTH & CHEM BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 164 EP AGFD PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08000166 ER PT J AU SZABO, A AF SZABO, A TI NMR RELAXATION, FLUORESCENCE DEPOLARIZATION AND THE DYNAMICS OF PROTEINS AND MEMBRANES - THEORY AND EXPERIMENT SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIH,CHEM PHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 166 EP PHYS PN 2 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG081 UT WOS:A1991HG08100701 ER PT J AU HU, Y LI, G YEH, HJC ZIFFER, H AF HU, Y LI, G YEH, HJC ZIFFER, H TI MICROBIAL HYDROXYLATION OF DIHYDROARTEMISININ DERIVATIVES SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 170 EP BIOT PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08000676 ER PT J AU LEEHUANG, S HUANG, PL NARA, PL CHEN, HC KUNG, HF HUANG, P HUANG, HI HUANG, PL AF LEEHUANG, S HUANG, PL NARA, PL CHEN, HC KUNG, HF HUANG, P HUANG, HI HUANG, PL TI MAP-30 - A NEW PLANT-DERIVED ANTI-HIV AGENT SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NYU,SCH MED,NEW YORK,NY 10016. NCI,FCRDC,FREDERICK,MD 21701. NICHHD,BETHESDA,MD 20892. AMER BIOSCI INC,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 190 EP AGFD PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08000192 ER PT J AU GUSTAFSON, KR CARDELLINA, JH MCMAHON, JB GULAKOWSKI, RJ BOYD, MR AF GUSTAFSON, KR CARDELLINA, JH MCMAHON, JB GULAKOWSKI, RJ BOYD, MR TI AIDS-ANTIVIRAL NATURAL-PRODUCTS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,DEV THERAPEUT PROGRAM,DRUG DISCOVERY RES & DEV LAB,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 192 EP AGFD PN 1 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG080 UT WOS:A1991HG08000194 ER PT J AU KUMAR, S KOLE, PL BALANI, SK JERINA, DM AF KUMAR, S KOLE, PL BALANI, SK JERINA, DM TI SYNTHESIS OF ENANTIOMERICALLY PURE BAY-REGION 10,11-DIOL-8,9-EPOXIDE DIASTEREOMERS OF CARCINOGENIC DIBENZ[A,H]ACRIDINE (DB[A,H]ACR) SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIDDKD,LBC,BETHESDA,MD 20892. SUNY COLL BUFFALO,DEPT ENVIRONM TOXICOL & CHEM,BUFFALO,NY 14222. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 25 PY 1991 VL 202 BP 208 EP ORGN PN 2 PG 0 WC Chemistry, Multidisciplinary SC Chemistry GA HG081 UT WOS:A1991HG08100301 ER PT J AU KAHN, RA AF KAHN, RA TI FLUORIDE IS NOT AN ACTIVATOR OF THE SMALLER (20-25 KDA) GTP-BINDING PROTEINS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Note ID STIMULATORY REGULATORY COMPONENT; GUANINE-NUCLEOTIDE-BINDING; ADP-RIBOSYLATION FACTOR; ADENYLATE-CYCLASE; BOVINE BRAIN; ESCHERICHIA-COLI; GAMMA-PHOSPHATE; ALPHA-SUBUNIT; CHOLERA-TOXIN; BETA-SUBUNIT AB Effects of aluminum, magnesium, and fluoride (AMF) on members of both the trimeric G protein and smaller (20-25 kDa) monomeric GTP-binding protein families were examined. The dissociation of GDP from G proteins was blocked by AMF but was unchanged with the addition of AMF to any of six of the monomeric GTP-binding proteins. Biochemical activities and properties of one of the smaller GTP-binding proteins, ADP-ribosylation factor, were also found to be unaffected by AMF. It is concluded that the ability of AMF to activate the trimeric G proteins is not shared by the smaller GTP-binding proteins and thus should prove to be a useful discriminator between cellular activities regulated by these two families of regulatory proteins. RP NCI, DIV CANC TREATMENT, BIOL CHEM LAB, BETHESDA, MD 20892 USA. NR 29 TC 259 Z9 260 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 25 PY 1991 VL 266 IS 24 BP 15595 EP 15597 PG 3 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB977 UT WOS:A1991GB97700011 PM 1908456 ER PT J AU BORELLINI, F HE, YF AQUINO, A YU, G JOSEPHS, SF GLAZER, RI AF BORELLINI, F HE, YF AQUINO, A YU, G JOSEPHS, SF GLAZER, RI TI INCREASED DNA-BINDING AND TRANSCRIPTIONAL ACTIVITY ASSOCIATED WITH TRANSCRIPTION FACTOR-SP1 IN K562 CELLS TRANSFECTED WITH THE MYELOID-SPECIFIC C-FES TYROSINE KINASE GENE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HL-60 LEUKEMIA-CELLS; NF-KAPPA-B; DEPENDENT PROTEIN-KINASE; ONCOGENE EXPRESSION; CELLULAR ONCOGENES; GROWTH-FACTORS; MESSENGER-RNA; DIFFERENTIATION; ACTIVATION; IDENTIFICATION AB Myeloblast cell line K562, when stably transfected with the human genomic c-fes sequence encoding a proto-oncogene tyrosine-protein kinase, acquires the characteristics of more mature granulocytic cells (WS-1 cells) and the ability to undergo differentiation (Yu, G., Smithgall, T. E., and Glazer, R. I. (1989) J. Biol. Chem. 264, 10276-1028 1). To explore the role of transcription factors in the differentiation process, WS-1 cells were analyzed for the presence of DNA-binding proteins capable of interacting with the 5'-long terminal repeat (LTR) region of human immunodeficiency virus (HIV)-l, that contains the binding sequences for transcription factors Sp1 and NF-kappa-B. Southwestern blotting and mobility shift assays revealed the presence of Sp1 in K562 and WS-1 cells. The DNA-binding activity of Sp1 was significantly greater in WS-1 cells than in K562 cells, despite the detection by immunoblotting of equivalent quantities and degrees of heterogeneity of Spl in both cell lines. DNA footprinting of the HIV-1 5'-LTR demonstrated that two of the three Sp1-binding sites and both NF-kappa-B binding sequences were protected by nuclear extracts from WS-1 cells, while no protection was afforded by nuclear extracts from K562 cells. Analysis of transcription in vitro by primer extension revealed enhanced initiation of transcription from the HIV-1 5'-LTR by nuclear extracts from WS-1 cells, but not from K562 cells. These data indicate that the response evoked by the c-fes tyrosine-protein kinase leads to enhanced DNA binding activity of Sp1 and NFKB, that results in the activation of transcription from the HIV-1 5'-LTR. C1 NCI,BIOL CHEM LAB,BALTIMORE,MD 21211. NCI,TUMOR CELL BIOL LAB,BALTIMORE,MD 21211. GEORGETOWN UNIV,MED CTR,VINCENT T LOMBARDI CANC RES CTR,WASHINGTON,DC 20007. GEORGETOWN UNIV,MED CTR,DEPT PHARMACOL,WASHINGTON,DC 20007. NR 46 TC 37 Z9 37 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 25 PY 1991 VL 266 IS 24 BP 15850 EP 15854 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB977 UT WOS:A1991GB97700051 PM 1874737 ER PT J AU BAI, R PAULL, KD HERALD, CL MALSPEIS, L PETTIT, GR HAMEL, E AF BAI, R PAULL, KD HERALD, CL MALSPEIS, L PETTIT, GR HAMEL, E TI HALICHONDRIN-B AND HOMOHALICHONDRIN-B, MARINE NATURAL-PRODUCTS BINDING IN THE VINCA DOMAIN OF TUBULIN - DISCOVERY OF TUBULIN-BASED MECHANISM OF ACTION BY ANALYSIS OF DIFFERENTIAL CYTOTOXICITY DATA SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ANTINEOPLASTIC AGENTS; PROTEIN PHOSPHATASE-1; PHOMOPSIN-A; SITE; POLYMERIZATION; NUCLEOTIDE; DOLASTATIN-10; DISTINCT; CYCLE AB Data generated in the new National Cancer Institute drug evaluation program, which is based on inhibition of cell growth in 60 human tumor cell lines, were used to compare new compounds with agents of known mechanism of action in terms of their differential cytotoxicity. Two marine natural products, halichondrin B and homohalichondrin B, appeared repeatedly when the data base was probed with known antimitotic agents. We confirmed that both compounds were highly cytotoxic (IC50 values for L1210 murine leukemia cells of 0.3 and 1 nM, respectively), with accumulation of cells arrested in mitosis at toxic concentrations, that both inhibited the polymerization of purified tubulin, and that both inhibited microtubule assembly dependent on microtubule-associated proteins. Limited amounts of homohalichondrin B, the less active agent, were available, so only halichondrin B was studied in detail. Halichondrin B did not interfere with colchicine binding to tubulin, but it was a noncompetitive inhibitor of the binding of vinblastine to tubulin (apparent K(i), 5.0-mu-M). Halichondrin B was therefore compared with other agents which interfere with the binding of vinca alkaloids to tubulin (vinblastine, maytansine, dolastatin 10, phomopsin A, rhizoxin) in terms of its effects on tubulin polymerization, inhibition of GTP hydrolysis, inhibition of nucleotide exchange, and stabilization of tubulin, as well as the quantitative assessment of its effects on vinca alkaloid binding and inhibition of cell growth. Since halichondrin B was originally isolated from the same organism as the phosphatase inhibitor okadaic acid, and since it is about 50-fold more effective than okadaic acid as an inhibitor of L1210 cell growth, perturbations of cellular microtubules observed following treatment with okadaic acid should be interpreted cautiously. C1 NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MOLEC PHARMACOL LAB,BLDG 37,RM 5C25,BETHESDA,MD 20892. NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,INFORMAT TECHNOL BRANCH,ROCKVILLE,MD 20892. ARIZONA STATE UNIV,CANC RES INST,TEMPE,AZ 85287. ARIZONA STATE UNIV,DEPT CHEM,TEMPE,AZ 85287. NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,FREDERICK,MD 21701. FU NCI NIH HHS [CA-44344-01A1-02]; PHS HHS [16049-10-12] NR 27 TC 264 Z9 274 U1 2 U2 23 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 25 PY 1991 VL 266 IS 24 BP 15882 EP 15889 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB977 UT WOS:A1991GB97700056 PM 1874739 ER PT J AU AOTA, S NAGAI, T YAMADA, KM AF AOTA, S NAGAI, T YAMADA, KM TI CHARACTERIZATION OF REGIONS OF FIBRONECTIN BESIDES THE ARGININE-GLYCINE-ASPARTIC ACID SEQUENCE REQUIRED FOR ADHESIVE FUNCTION OF THE CELL-BINDING DOMAIN USING SITE-DIRECTED MUTAGENESIS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HUMAN-PLASMA FIBRONECTIN; CYTOSKELETAL ORGANIZATION; MONOCLONAL-ANTIBODIES; FRAGMENTS; ATTACHMENT; IDENTIFICATION; FIBROBLASTS; MOLECULE AB Previous studies of adhesion mediated by the central cell-binding domain of fibronectin suggest that additional polypeptide information besides the Arg-Gly-Asp sequence is required for full activity. We analyzed this putative second, synergistic region of fibronectin more extensively by deletion analysis and oligonucleotide-based site-directed mutagenesis. Resulting mutated fusion proteins expressed using lambda-gt11 were assayed for baby hamster kidney fibroblast cell spreading activity. Deletion mutants truncating from the amino terminus showed a decrease of activity in two apparently discrete steps. Complementary studies using a series of overlapped internal deletions designed to retain the repetitive fibronectin structure also indicated that two distinct peptide regions besides the RGD sequence were necessary for full activity. Removal of the carboxyl-terminal region resulted in the greatest loss of activity (greater-than-or-equal-to 20- versus 3-5-fold). Very similar results were obtained with HT-1080 cells dependent on the alpha-5-beta-1 integrin receptor for adhesion to fibronectin. An anti-fibronectin monoclonal antibody that inhibits cell adhesion was found to bind to the carboxyl-terminal functional region, and a point mutation caused specific loss of its epitope. These studies reveal unexpected complexity in the organization of these functional regions, which contrasts with adhesion models based only on simple, short peptide recognition sequences. C1 NIDR,DEV BIOL LAB,BLDG 30,RM 414,BETHESDA,MD 20892. OI Yamada, Kenneth/0000-0003-1512-6805 NR 25 TC 160 Z9 161 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 25 PY 1991 VL 266 IS 24 BP 15938 EP 15943 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB977 UT WOS:A1991GB97700063 PM 1874740 ER PT J AU DEUTSCH, D PALMON, A FISHER, LW KOLODNY, N TERMINE, JD YOUNG, MF AF DEUTSCH, D PALMON, A FISHER, LW KOLODNY, N TERMINE, JD YOUNG, MF TI SEQUENCING OF BOVINE ENAMELIN (TUFTELIN) A NOVEL ACIDIC ENAMEL PROTEIN SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID RAT INCISOR ENAMEL; MATRIX PROTEINS; IMMUNOHISTOCHEMICAL LOCALIZATION; FETAL ENAMEL; TOOTH; BIOSYNTHESIS; AMELOGENINS; HYDROLYSIS; PEPTIDES; ALBUMIN AB Enamelins are a major group of 28-70-kDa acidic proteins rich in aspartic acid, glutamic acid, serine, and glycine found in developing and mature extracellular enamel; a unique and highly mineralized ectodermal tissue covering vertebrate teeth. They have been associated with the mineralization and structural organization of this tissue. In an attempt to elucidate the primary structure of enamelin, a 2674-base pair cDNA isolated from a bovine ameloblast-enriched, lambda Zap 2 expression library, was sequenced. The identity and localization of the deduced protein was confirmed by amino acid composition, enzyme-linked immunosorbent assay, Western blotting, indirect immunohistochemistry, and high resolution protein-A gold immunocytochemistry. The immunological techniques were employed using antibodies directed against synthetic peptides corresponding to the protein sequence deduced from the cloned cDNA sequence. The results reveal the deduced protein to be a novel acidic enamel protein. It contains 389 amino acids and has a calculated molecular weight of 43,814. Its amino acid composition is similar to that of "tuft" proteins (enamel matrix protein fragments remaining in the mature tissue). It contains one potential N-glycosylation site and 5 cysteine residues. Southern hybridization of the cloned cDNA with genomic bovine DNA indicated the existence of a single gene with one or more introns. C1 NIDR,BONE RES BRANCH,BETHESDA,MD 20892. NIDR,CELLULAR DEV & ONCOL LAB,BETHESDA,MD 20892. RP DEUTSCH, D (reprint author), HEBREW UNIV JERUSALEM,HADASSAH FAC DENT MED,DENT RES UNIT,POB 1172,JERUSALEM,ISRAEL. FU NIDCR NIH HHS [R0I-DEO-5780] NR 53 TC 112 Z9 118 U1 0 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 25 PY 1991 VL 266 IS 24 BP 16021 EP 16028 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB977 UT WOS:A1991GB97700075 PM 1874744 ER PT J AU RIBEIRO, P PIGEON, D KAUFMAN, S AF RIBEIRO, P PIGEON, D KAUFMAN, S TI THE HYDROXYLATION OF PHENYLALANINE AND TYROSINE BY TYROSINE-HYDROXYLASE FROM CULTURED PHEOCHROMOCYTOMA CELLS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID CYCLIC-AMP; PURIFICATION; BRAIN; ACTIVATION; SUBSTRATE; HOMOLOGY; AGENTS; LINE AB Pheochromocytoma tyrosine hydroxylase was reported to have unusual catalytic properties, which might be unique to the tumor enzyme (Dix, T. A., Kuhn, D. M., and Benkovic, S. J. (1987) Biochemistry 24, 3354-3361). Two such properties, namely the apparent inability to hydroxylate phenylalanine and an unprecedented reactivity with hydrogen peroxide were investigated further in the present study. Tyrosine hydroxylase was purified to apparent homogeneity from cultured pheochromocytoma PC12 cells. The purified tumor enzyme was entirely dependent on tetrahydrobiopterin (BH4) for the hydroxylation of tyrosine to 3,4-dihydroxyphenylalanine and hydrogen peroxide could not substitute for the natural cofactor. Indeed, in the presence of BH4, increasing concentrations of hydrogen peroxide completely inhibited enzyme activity. The PC12 hydroxylase exhibited typical kinetics of tyrosine hydroxylation, both as a function of tyrosine (s0.5 Tyr = 15-mu-M) and BH4 (apparent K(m) BH4 = 210-mu-M). In addition, the enzyme catalyzed the hydroxylation of substantial amounts of phenylalanine to tyrosine and 3,4-dihydroxyphenylalanine (apparent K(m) Phe = 100-mu-M). Phenylalanine did not inhibit the enzyme in the concentrations tested, whereas tyrosine showed typical substrate inhibition at concentrations greater-than-or-equal-to 50-mu-M. At higher substrate concentrations, the rate of phenylalanine hydroxylation was equal to or exceeded that of tyrosine. Essentially identical results were obtained with purified tyrosine hydroxylase from pheochromocytoma PC18 cells. The data suggest that the tumor enzyme has the same substrate specificity and sensitivity to hydrogen peroxide as tyrosine hydroxylase from other tissues. RP RIBEIRO, P (reprint author), NIMH,NEUROCHEM LAB,BETHESDA,MD 20892, USA. NR 24 TC 36 Z9 36 U1 1 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 25 PY 1991 VL 266 IS 24 BP 16207 EP 16211 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB977 UT WOS:A1991GB97700101 PM 1678741 ER PT J AU HATTA, M MIYATANI, S COPELAND, NG GILBERT, DJ JENKINS, NA TAKEICHI, M AF HATTA, M MIYATANI, S COPELAND, NG GILBERT, DJ JENKINS, NA TAKEICHI, M TI GENOMIC ORGANIZATION AND CHROMOSOMAL MAPPING OF THE MOUSE P-CADHERIN GENE SO NUCLEIC ACIDS RESEARCH LA English DT Article ID CELL-ADHESION MOLECULE; CYTOPLASMIC DOMAIN; L-CAM; UVOMORULIN; CDNA; FAMILY; IDENTIFICATION; LOCALIZATION; LINKAGE; REGION AB Cadherins are a family of Ca2+-dependent cell adhesion molecules, that includes P-cadherin, E-cadherin, N-cadherin and L-CAM. In this study, the genomic organization of the mouse P-cadherin gene was determined by analyzing overlapping DNA clones obtained from a mouse genomic library. The results showed that this gene spans over 45kb and consists of 15 exons. A marked feature of this gene is that the first intron is 23kbp long accounting for half its length. Comparisons of this structure with that of L-CAM, a chicken cadherin, revealed that the exon-intron boundaries are conserved between the two genes except that the P-cadherin first exon includes the corresponding first and second exons of the L-CAM gene. This gene was also similar to the other in that the second intron, which corresponds to the P-cadherin first intron, is exceptionally longer than other introns. These results suggest that the exon-intron pattern conserved in these genes is of significance for generation of domain structure of cadherin molecules or for their transcriptional regulation. We also determined the chromosomal localization of the P-cadherin gene by interspecific backcross analysis, and found that this gene is located in the central region of mouse chromosome 8 and linked with the E-cadherin locus. This is the first evidence for the linkage of different cadherin genes. C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21701. RP TAKEICHI, M (reprint author), KYOTO UNIV,FAC SCI,DEPT BIOPHYS,SAKYO KU,KYOTO 606,JAPAN. RI Takeichi, Masatoshi/G-5903-2012 OI Takeichi, Masatoshi/0000-0002-9931-3378 FU NCI NIH HHS [N01-CO-74101] NR 30 TC 66 Z9 67 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4437 EP 4441 DI 10.1093/nar/19.16.4437 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300015 PM 1886768 ER PT J AU SANDER, M LOWENHAUPT, K LANE, WS RICH, A AF SANDER, M LOWENHAUPT, K LANE, WS RICH, A TI CLONING AND CHARACTERIZATION OF RRP1, THE GENE ENCODING DROSOPHILA STRAND TRANSFERASE - CARBOXY-TERMINAL HOMOLOGY TO DNA-REPAIR ENDO EXONUCLEASES SO NUCLEIC ACIDS RESEARCH LA English DT Article ID ESCHERICHIA-COLI; SACCHAROMYCES-CEREVISIAE; HUMAN-CELLS; APURINIC ENDONUCLEASE; CHROMOSOME SYNAPSIS; SEQUENCE-ANALYSIS; DAMAGING AGENTS; MEIOTIC CELLS; RECOMBINATION; MUTANTS AB We previously reported the purification of a protein from Drosophila embryo extracts that carries out the strand transfer step in homologous recombination (Lowenhaupt, K., Sander, M., Hauser, C. and A. Rich, 1989, J. Biol. Chem. 264, 20568). We report here the isolation of the gene encoding this protein. Partial amino acid sequence from a tryptic digest of gel purified strand transfer protein was used to design a pair of degenerate oligonucleotide primers which amplified a 635 bp region of Drosophila genomic DNA. Recombinant bacteriophage were isolated from genomic and embryo cDNA libraries by screening with the amplified DNA fragment. These bacteriophage clones identify a single copy gene that expresses a single mRNA transcript in early embryos and in embryo-derived tissue culture cells. The cDNA nucleotide sequence contains an open reading frame of 679 amino acids within which are found 5 tryptic peptides from the strand transfer protein. Expression of this cDNA in E. coli produces a polypeptide with the same electrophoretic mobility as the purified protein. The deduced protein sequence has two distinct regions. The first 427 residues are basic, rich in glutamic acid and lysine residues and unrelated to known proteins. The carboxy-terminal 252 residues are average in amino acid composition and are homologous to the DNA repair proteins, Escherichia coli exonuclease III and Streptococcus pneumoniae exonuclease A. This protein, which we name Rrp1 (Recombination Repair Protein 1), may facilitate recombinational repair of DNA damage. C1 HARVARD UNIV,HARVARD MICROCHEM FACIL,CAMBRIDGE,MA 02138. MIT,DEPT BIOL,CAMBRIDGE,MA 02139. RP SANDER, M (reprint author), NIEHS,GENET LAB D304,RES TRIANGLE PK,NC 27709, USA. NR 45 TC 43 Z9 44 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4523 EP 4529 DI 10.1093/nar/19.16.4523 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300028 PM 1653418 ER PT J AU DELISIO, J LATIF, F GLENN, GM YAO, M GEIL, L ZBAR, B LERMAN, MI AF DELISIO, J LATIF, F GLENN, GM YAO, M GEIL, L ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB46-27' (D3S635) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74102] NR 2 TC 1 Z9 1 U1 0 U2 5 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4564 EP 4564 DI 10.1093/nar/19.16.4564-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300046 PM 1886785 ER PT J AU LI, H LATIF, F TORY, K YAO, M ORCUTT, ML ZBAR, B LERMAN, MI AF LI, H LATIF, F TORY, K YAO, M ORCUTT, ML ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB17-11 (D3S1092) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74102] NR 2 TC 1 Z9 1 U1 0 U2 5 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4564 EP 4564 DI 10.1093/nar/19.16.4564 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300045 PM 1715984 ER PT J AU LATIF, F GLENN, GM GEIL, L RAYFORD, W DELISIO, J ZBAR, B LERMAN, MI AF LATIF, F GLENN, GM GEIL, L RAYFORD, W DELISIO, J ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB45-86 (D3S633) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NR 2 TC 1 Z9 1 U1 0 U2 10 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4565 EP 4565 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300048 PM 1886787 ER PT J AU LATIF, F GLENN, GM GEIL, L RAYFORD, W DELISIO, J ZBAR, B LERMAN, MI AF LATIF, F GLENN, GM GEIL, L RAYFORD, W DELISIO, J ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB27-98'' (D3S1096) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NR 2 TC 1 Z9 1 U1 0 U2 10 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4565 EP 4565 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300047 PM 1886787 ER PT J AU LATIF, F GLENN, GM GEIL, L RAYFORD, W YAO, M DELISIO, J ZBAR, B LERMAN, MI AF LATIF, F GLENN, GM GEIL, L RAYFORD, W YAO, M DELISIO, J ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB27-77 (D3S603) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NR 2 TC 1 Z9 1 U1 0 U2 10 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4566 EP 4566 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300050 PM 1886788 ER PT J AU LATIF, F GLENN, GM GEIL, L RAYFORD, W DELISIO, J ZBAR, B LERMAN, MI AF LATIF, F GLENN, GM GEIL, L RAYFORD, W DELISIO, J ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB39-63 (D3S616) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NR 2 TC 1 Z9 1 U1 0 U2 10 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4566 EP 4566 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300049 PM 1886788 ER PT J AU LATIF, F GLENN, GM GEIL, L DELISIO, J ZBAR, B LERMAN, MI AF LATIF, F GLENN, GM GEIL, L DELISIO, J ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME 3P - LAMBDA-LIB37-96' (D3S1192) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NR 2 TC 1 Z9 1 U1 0 U2 10 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4567 EP 4567 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300052 PM 1715986 ER PT J AU LATIF, F GLENN, GM GEIL, L RAYFORD, W DELISIO, J ZBAR, B LERMAN, MI AF LATIF, F GLENN, GM GEIL, L RAYFORD, W DELISIO, J ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB13-67 (D3S591) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NR 2 TC 1 Z9 1 U1 0 U2 10 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4567 EP 4567 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300051 PM 1715986 ER PT J AU DELISIO, J LATIF, F GLENN, GM GEIL, L ZBAR, B LERMAN, MI AF DELISIO, J LATIF, F GLENN, GM GEIL, L ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB50-50' (D3S1099) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NR 2 TC 1 Z9 1 U1 0 U2 10 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4568 EP 4568 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300054 PM 1715987 ER PT J AU DELISIO, J LATIF, F GLENN, GM GEIL, L ZBAR, B LERMAN, MI AF DELISIO, J LATIF, F GLENN, GM GEIL, L ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB4-59 (D3S575) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NR 2 TC 1 Z9 1 U1 0 U2 10 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4568 EP 4568 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300053 PM 1715987 ER PT J AU GEIL, L LATIF, F YAO, M ORCUTT, ML ZBAR, B LERMAN, MI AF GEIL, L LATIF, F YAO, M ORCUTT, ML ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB36-68 (D3S615) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74102] NR 2 TC 0 Z9 0 U1 0 U2 5 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4569 EP 4569 DI 10.1093/nar/19.16.4569 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300055 PM 1886791 ER PT J AU POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR AF POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR TI TRINUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN GAMMA-B-CRYSTALLIN GENE SO NUCLEIC ACIDS RESEARCH LA English DT Note RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 4 TC 3 Z9 3 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4571 EP 4571 DI 10.1093/nar/19.16.4571 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300059 PM 1886794 ER PT J AU WEI, M LATIF, F TORY, K YAO, M ORCUTT, ML ZBAR, B LERMAN, MI AF WEI, M LATIF, F TORY, K YAO, M ORCUTT, ML ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB18-88 (D3S1093) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,FREDERICK CANC RES & DEV CTR,IMMUNOBIOL LAB,BLDG 560,ROOM 12-71,FREDERICK,MD 21701. NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,DYNCORP,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74102] NR 2 TC 1 Z9 1 U1 0 U2 5 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 25 PY 1991 VL 19 IS 16 BP 4575 EP 4575 DI 10.1093/nar/19.16.4575 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GD893 UT WOS:A1991GD89300067 PM 1715988 ER PT J AU WIKTOR, SZ PATE, EJ WEISS, SH GOHD, RS CORREA, P FONTHAM, ET HANCHARD, B BIGGAR, RJ BLATTNER, WA AF WIKTOR, SZ PATE, EJ WEISS, SH GOHD, RS CORREA, P FONTHAM, ET HANCHARD, B BIGGAR, RJ BLATTNER, WA TI SENSITIVITY OF HTLV-I ANTIBODY-ASSAYS FOR HTLV-II SO LANCET LA English DT Letter C1 UNIV W INDIES,KINGSTON 7,JAMAICA. UNIV MED & DENT NEW JERSEY,NEWARK,NJ 07103. LOUISIANA STATE UNIV,MED CTR,SCH MED,NEW ORLEANS,LA 70112. RP WIKTOR, SZ (reprint author), NCI,BETHESDA,MD 20892, USA. NR 6 TC 34 Z9 34 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD AUG 24 PY 1991 VL 338 IS 8765 BP 512 EP 513 DI 10.1016/0140-6736(91)90585-D PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA GC153 UT WOS:A1991GC15300043 PM 1678470 ER PT J AU GULYA, K DAVE, JR HOFFMAN, PL AF GULYA, K DAVE, JR HOFFMAN, PL TI CHRONIC ETHANOL INGESTION DECREASES VASOPRESSIN MESSENGER-RNA IN HYPOTHALAMIC AND EXTRAHYPOTHALAMIC NUCLEI OF MOUSE-BRAIN SO BRAIN RESEARCH LA English DT Article DE HYPOTHALAMIC VASOPRESSIN MESSENGER RNA, EFFECT OF ETHANOL; INSITU HYBRIDIZATION; VASOPRESSIN; EXTRAHYPOTHALAMIC VASOPRESSIN MESSENGER RNA; CHRONIC ETHANOL TREATMENT ID INSITU HYBRIDIZATION HISTOCHEMISTRY; STRIA TERMINALIS; BED NUCLEUS; SEX-DIFFERENCES; LATERAL SEPTUM; C-FOS; RAT; OXYTOCIN; NEURONS; CELLS AB Endogenous arginine vasopressin was previously shown to modulate the rate of loss of functional (CNS) tolerance to ethanol, suggesting that chronic ethanol ingestion might alter vasopressin synthesis and/or release. Since extrahypothalamic vasopressin is believed to be involved in the CNS effects of the peptide, we determined the effect of ethanol on vasopressin mRNA in the bed nucleus of the stria terminalis (BST), as well as in several hypothalamic nuclei. Chronic ethanol ingestion, that produced functional tolerance and physical dependence in mice, resulted in decreased vasopressin mRNA levels in all areas examined. In contrast, as expected, dehydration resulted in increases in vasopressin mRNA in the BST and in all hypothalamic nuclei except the suprachiasmatic nucleus. In the BST, both ethanol ingestion and dehydration affected cells in the central region of the nucleus, while cells in the caudal portion were only affected by ethanol treatment. The results indicate that chronic ethanol ingestion generally reduces the synthesis of vasopressin, and that increased vasopressin synthesis is not necessary in order for the peptide to affect ethanol tolerance. C1 NIAAA,DIV INTRAMURAL CLIN & BIOL RES,ROCKVILLE,MD 20852. NR 39 TC 20 Z9 20 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD AUG 23 PY 1991 VL 557 IS 1-2 BP 129 EP 135 DI 10.1016/0006-8993(91)90125-F PG 7 WC Neurosciences SC Neurosciences & Neurology GA GE520 UT WOS:A1991GE52000018 PM 1747746 ER PT J AU MURPHY, VA EMBREY, EC ROSENBERG, JM SMITH, QR RAPOPORT, SI AF MURPHY, VA EMBREY, EC ROSENBERG, JM SMITH, QR RAPOPORT, SI TI CALCIUM DEFICIENCY ENHANCES CADMIUM ACCUMULATION IN THE CENTRAL-NERVOUS-SYSTEM SO BRAIN RESEARCH LA English DT Article DE CADMIUM; CALCIUM DEFICIENT; BRAIN; SERUM; LIVER; KIDNEY; FEMUR ID CHRONIC HYPOCALCEMIA; CEREBROSPINAL-FLUID; RATS; BRAIN; TOXICITY; CD-109; TISSUE AB Weanling male rats were administered 1 of 4 diets for 40 days: control (CONT), low Ca (LOCA), control plus Cd (CONT + Cd) or low Ca plus Cd (LOCA + Cd). After 40 days, Cd was analyzed in 7 brain regions, spinal cord, serum, liver, kidney, muscle and femur by atomic absorption spectrophotometry with Zeeman background correction. No significant difference in Cd between CONT and LOCA was found except in femur, where it was increased. In CONT + Cd rats, peripheral tissues showed an increase in Cd of 30-71 fold above CONT rats. Brain regions exhibited a more modest 7-10 fold change, and serum Cd was 8.5 times above control. LOCA + Cd rats showed a 25-fold increase of Cd above LOCA in serum, 25-100 fold in peripheral tissues, and a 14-20 times increase in brain. These findings show that brain Cd is increased during Ca deficiency, but that central nervous system Cd changes less than peripheral tissue Cd. This increase in brain Cd could alter brain function. RP MURPHY, VA (reprint author), NIA,NEUROSCI LAB,BLDG 10,ROOM 6C103,BETHESDA,MD 20892, USA. NR 19 TC 7 Z9 8 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD AUG 23 PY 1991 VL 557 IS 1-2 BP 280 EP 284 DI 10.1016/0006-8993(91)90144-K PG 5 WC Neurosciences SC Neurosciences & Neurology GA GE520 UT WOS:A1991GE52000037 PM 1747758 ER PT J AU OLIVER, AE DEAMER, DW AKESON, M AF OLIVER, AE DEAMER, DW AKESON, M TI EVIDENCE THAT SENSITIVITY TO STEROID ANESTHETICS APPEARS LATE IN EVOLUTION SO BRAIN RESEARCH LA English DT Note DE ANESTHESIA; GAMMA-AMINOBUTYRIC ACID; GAMMA-AMINOBUTYRIC ACID RECEPTOR; STEROID ANESTHETIC; PREGNANOLONE; 5-BETA-PREGNANE-3-ALPHA-OH-20-ONE ID APLYSIA BUCCAL GANGLIA; CONDUCTANCE; RECEPTOR AB The effects of pregnanolone, a steroid anesthetic, were compared with diethyl ether and short chain alkanols in 21 aquatic species from 7 phyla. Loss of righting reflex and escape response were used as indicators of anesthesia. All organisms were anesthetized by diethyl ether and short chain alkanols, but pregnanolone affected only organisms belonging to the phylum Chordata. It is probable that pregnanolone exerts its effect on the gamma-aminobutyric acid (GABA) receptor. Because many invertebrates do possess GABA receptors, our results suggest that a binding site at which steroid binding causes organismal anesthesia appeared early in chordate evolution on a previously existing GABA receptor. The results also appear to exclude a primary lipid bilayer site for steroid anesthetic action. C1 NIMH,MOLEC BIOL LAB,BETHESDA,MD 20892. RP OLIVER, AE (reprint author), UNIV CALIF DAVIS,DEPT ZOOL,DAVIS,CA 95616, USA. NR 19 TC 14 Z9 14 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD AUG 23 PY 1991 VL 557 IS 1-2 BP 298 EP 302 DI 10.1016/0006-8993(91)90148-O PG 5 WC Neurosciences SC Neurosciences & Neurology GA GE520 UT WOS:A1991GE52000041 PM 1747761 ER PT J AU ICHIKAWA, H JACOBOWITZ, DM WINSKY, L HELKE, CJ AF ICHIKAWA, H JACOBOWITZ, DM WINSKY, L HELKE, CJ TI CALRETININ-IMMUNOREACTIVITY IN VAGAL AND GLOSSOPHARYNGEAL SENSORY NEURONS OF THE RAT - DISTRIBUTION AND COEXISTENCE WITH PUTATIVE TRANSMITTER AGENTS SO BRAIN RESEARCH LA English DT Note DE CALRETININ; CALCITONIN GENE-RELATED PEPTIDE; SUBSTANCE-P; TYROSINE HYDROXYLASE; VAGAL; GLOSSOPHARYNGEAL; SENSORY NEURON; IMMUNOHISTOCHEMISTRY ID CALCIUM-BINDING PROTEIN; GENE-RELATED PEPTIDE; SUBSTANCE-P; IMMUNOHISTOCHEMICAL LOCALIZATION; PARVALBUMIN; INNERVATION; NERVES; NEUROTRANSMITTER; NEUROPEPTIDES; EXPRESSION AB Immunoreactivity for the calcium binding protein, calretinin (calretinin-ir), was demonstrated in cell bodies of vagal and glossopharyngeal sensory ganglia (jugular, petrosal, and nodose ganglia) and in associated nerve fibers. In the jugular and petrosal ganglia, many calretinin-ir neurons were also immunoreactive for calcitonin gene-related peptide and substance P. In the nodose ganglion, most of the calretinin-ir neurons lacked these peptides. None of the calretinin-ir neurons in these ganglia were also immunoreactive for tyrosine hydroxylase. C1 UNIFORMED SERV UNIV HLTH SCI,DEPT PHARMACOL,4301 JONES BRIDGE RD,BETHESDA,MD 20814. NIMH,CLIN SCI LAB,BETHESDA,MD 20892. FU NINDS NIH HHS [R01 NS20991] NR 36 TC 48 Z9 51 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD AUG 23 PY 1991 VL 557 IS 1-2 BP 316 EP 321 DI 10.1016/0006-8993(91)90152-L PG 6 WC Neurosciences SC Neurosciences & Neurology GA GE520 UT WOS:A1991GE52000045 PM 1720997 ER PT J AU MEFFORD, IN MASTERS, CF HEYES, MP ESKAY, RL AF MEFFORD, IN MASTERS, CF HEYES, MP ESKAY, RL TI CYTOKINE-INDUCED ACTIVATION OF THE NEUROENDOCRINE STRESS AXIS PERSISTS IN ENDOTOXIN-TOLERANT MICE SO BRAIN RESEARCH LA English DT Note DE LIPOPOLYSACCHARIDE; NEUROENDOCRINE STRESS AXIS; NORADRENALINE; CORTICOSTERONE; HYPOTHALAMUS ID TUMOR NECROSIS FACTOR; PITUITARY-ADRENAL AXIS; FACTOR-ALPHA; DESENSITIZATION; INTERLEUKIN-1; LIPOPOLYSACCHARIDE; NOREPINEPHRINE; HYPOTHALAMUS; INVOLVEMENT; INVIVO AB Chronic administration of lipopolysaccharide (LPS) to mice markedly reduced activation of the neuroendocrine stress axis elicited by an acute challenge dose of LPS. LPS-induced elevation in norepinephrine turnover in the hypothalamus showed complete tolerance whereas elevation of plasma corticosterone showed only partial tolerance. Challenge-induced increased turnover of dopamine in hypothalamus persisted in LPS-tolerant animals. Neuroendocrine activation persisted following acute challenge with interleukin-1 and tumor necrosis factor following chronic LPS exposure. C1 NIMH,CLIN SCI LAB,ANALYT BIOCHEM SECT,BETHESDA,MD 20892. NIAAA,CLIN STUDIES LAB,BETHESDA,MD 20892. RP MEFFORD, IN (reprint author), NIMH,CLIN PHARMACOL SECT,CLIN NEUROSCI BRANCH,BLDG 10,ROOM 2D46,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 21 TC 30 Z9 30 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD AUG 23 PY 1991 VL 557 IS 1-2 BP 327 EP 330 DI 10.1016/0006-8993(91)90154-N PG 4 WC Neurosciences SC Neurosciences & Neurology GA GE520 UT WOS:A1991GE52000047 PM 1747764 ER PT J AU WELLS, FB GAHM, SJ HEDRICK, SM BLUESTONE, JA DENT, A MATIS, LA AF WELLS, FB GAHM, SJ HEDRICK, SM BLUESTONE, JA DENT, A MATIS, LA TI REQUIREMENT FOR POSITIVE SELECTION OF GAMMA-DELTA-RECEPTOR-BEARING T-CELLS SO SCIENCE LA English DT Article ID LYMPHOCYTES-T; CYCLOSPORINE-A; STEM-CELLS; ANTIGEN; THYMUS; MICE; EXPRESSION; CD8; SPECIFICITY; RECOGNITION AB The alpha-beta and gamma-delta-T cell receptors for antigen (TCR) delineate distinct T cell populations. TCR-alpha-beta-bearing thymocytes must be positively selected by binding of the TCR to major histocompatibility complex (MHC) molecules on thymic epithelium. To examine the requirement for positive selection of TCR gamma-delta-T cells, mice bearing a class I MHC-specific gamma-delta-transgene (Tg) were crossed to mice with disrupted beta-2 microglobulin (beta-2M) genes. The Tg+beta-2M- (class I MHC-) offspring had Tg+ thymocytes that did not proliferate to antigen or Tg-specific monoclonal antibody and few peripheral Tg+ cells. This is evidence for positive selection within the gamma-delta-T cell subset. C1 NCI,FREDERICK CANC RES & DEV CTR,MOLEC IMMUNOREGULAT LAB,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21702. NCI,HOWARD HUGHES MED INST,RES SCHOLARS PROGRAM,BETHESDA,MD 20892. UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093. UNIV CALIF SAN DIEGO,CTR CANC,LA JOLLA,CA 92093. NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC DYNCORP,FREDERICK,MD 21701. UNIV CHICAGO,BEN MAY INST,DEPT PATHOL,COMM IMMUNOL,CHICAGO,IL 60637. FU NIAID NIH HHS [AI00602, AI 26847-03]; PHS HHS [N01-C0-74102] NR 32 TC 71 Z9 71 U1 0 U2 1 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD AUG 23 PY 1991 VL 253 IS 5022 BP 903 EP 905 DI 10.1126/science.1831565 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GC152 UT WOS:A1991GC15200045 PM 1831565 ER PT J AU MCDIARMID, R SABLJIC, A AF MCDIARMID, R SABLJIC, A TI ANALYSIS OF THE ABSORPTION-SPECTRUM OF THE 195-NM REGION OF CYCLOPENTADIENE SO JOURNAL OF PHYSICAL CHEMISTRY LA English DT Article AB The 195-nm region of the absorption spectra of cyclopentadiene and cyclopentadiene-d6 has been measured and analyzed. Two distinct 3p Rydberg electronic states are demonstrated to occur here. Their extensive vibrational structures are assigned as fundamentals, combinations, and overtones of the a1 C = C stretching, CH wagging, and ring deformation, the a2 CH bending, and the b1 C = C twisting vibrations. The displacements of the upper states from the ground along the C = C stretch and ring deformation coordinates are estimated from the absorption intensities. A ground-state fundamental frequency of 823 cm-1 is deduced for the a1 ring deformation mode in C5D6. The electronic lifetimes are estimated to be a minimum of 0.13 ps. The observed transition energies were used to assess the accuracy of ab initio calculations thereof. The results obtained here are compared with those previously observed for butadiene. C1 RUDJER BOSKOVIC INST,YU-41001 ZAGREB,YUGOSLAVIA. RP MCDIARMID, R (reprint author), NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892, USA. RI Sabljic, Aleksandar/D-4146-2011 NR 22 TC 17 Z9 17 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-3654 J9 J PHYS CHEM-US JI J. Phys. Chem. PD AUG 22 PY 1991 VL 95 IS 17 BP 6455 EP 6462 DI 10.1021/j100170a014 PG 8 WC Chemistry, Physical SC Chemistry GA GC370 UT WOS:A1991GC37000014 ER PT J AU SAFRIN, S CRUMPACKER, C CHATIS, P DAVIS, R HAFNER, R RUSH, J KESSLER, HA LANDRY, B MILLS, J AF SAFRIN, S CRUMPACKER, C CHATIS, P DAVIS, R HAFNER, R RUSH, J KESSLER, HA LANDRY, B MILLS, J TI A CONTROLLED TRIAL COMPARING FOSCARNET WITH VIDARABINE FOR ACYCLOVIR-RESISTANT MUCOCUTANEOUS HERPES-SIMPLEX IN THE ACQUIRED-IMMUNODEFICIENCY-SYNDROME SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID ADENINE-ARABINOSIDE THERAPY; VIRUS-INFECTIONS; DNA-POLYMERASE; IMMUNOCOMPROMISED PATIENT; AIDS; TYPE-2; THYMIDINE; TOXICITY; KINASE; HUMANS AB Background and Methods. Most strains of herpes simplex virus that are resistant to acyclovir are susceptible in vitro to both foscarnet and vidarabine. We conducted a randomized trial to compare foscarnet with vidarabine in 14 patients with the acquired immunodeficiency syndrome (AIDS) and mucocutaneous herpetic lesions that had been unresponsive to intravenous therapy with acyclovir for a minimum of 10 days. The patients were randomly assigned to receive either foscarnet (40 mg per kilogram of body weight intravenously every 8 hours) or vidarabine (15 mg per kilogram per day intravenously) for 10 to 42 days. In the isolates of herpes simplex virus we documented in vitro resistance to acyclovir and susceptibility to foscarnet and vidarabine. Results. The lesions in all eight patients assigned to foscarnet healed completely after 10 to 24 days of therapy. In contrast, vidarabine was discontinued because of failure in all six patients assigned to receive it. The time to complete healing (P = 0.01), time to 50 percent reductions in the size of the lesions (P = 0.01) and the pain score (P = 0.004), and time to the end of viral shedding (P = 0.006) were all significantly shorter in the patients assigned to foscarnet. Three patients had new neurologic abnormalities while receiving vidarabine. No patient discontinued foscarnet because of toxicity. Although initial recurrences of herpes simplex infection after the index lesion had healed tended to be susceptible to acyclovir, acyclovir-resistant infection eventually recurred in every healed patient, a median of 42.5 days (range, 14 to 191) after foscarnet was discontinued. Conclusions. For the treatment of acyclovir-resistant herpes simplex infection in patients with AIDS, foscarnet has superior efficacy and less frequent serious toxicity than vidarabine. Once the treatment is stopped, however, there is a high frequency of relapse. C1 NIAID,DIV AIDS,BETHESDA,MD 20892. RUSH MED COLL,DEPT MED,CHICAGO,IL 60612. RUSH MED COLL,DEPT IMMUNOL MICROBIOL,CHICAGO,IL 60612. HARVARD UNIV,SCH PUBL HLTH,CTR STAT & DATA ANAL,BOSTON,MA 02115. UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,DEPT EPIDEMIOL & BIOSTAT,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL,SAN FRANCISCO,CA 94143. BETH ISRAEL HOSP,DEPT MED,BOSTON,MA 02215. NR 30 TC 267 Z9 277 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD AUG 22 PY 1991 VL 325 IS 8 BP 551 EP 555 DI 10.1056/NEJM199108223250805 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA GB447 UT WOS:A1991GB44700005 PM 1649971 ER PT J AU DETERDING, LJ MOSELEY, MA TOMER, KB JORGENSEN, JW AF DETERDING, LJ MOSELEY, MA TOMER, KB JORGENSEN, JW TI NANOSCALE SEPARATIONS COMBINED WITH TANDEM MASS-SPECTROMETRY SO JOURNAL OF CHROMATOGRAPHY LA English DT Article; Proceedings Paper CT 7TH INTERNATIONAL SYMP ON LIQUID CHROMATOGRAPHY-MASS SPECTRUM CY OCT 31-NOV 02, 1990 CL MONTREUX, SWITZERLAND ID FAST-ATOM-BOMBARDMENT; CAPILLARY LIQUID-CHROMATOGRAPHY; BOROSILICATE GLASS-CAPILLARIES; REVERSED-PHASE COLUMNS; OPEN-TUBULAR COLUMNS; LIMITED PRESSURE; ANALYSIS TIME; OPTIMIZATION; BIOMOLECULES; FABRICATION AB High-efficiency separations of peptide mixtures, tryptic digests and other biological compounds have been achieved using nanoscale packed capillaries and capillary zone electrophoresis (CZE). The coaxial continuous-flow fast atom bombardment design is an excellent interface for coupling these separation techniques with mass spectrometry (MS). In addition, this interface is very useful for the acquisition of MS-MS data from compounds separated by nanoscale packed capillary liquid chromatography and CZE. Structurally informative daughter-ion spectra can be obtained at the low picomole to femtomole level. C1 UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27514. RP DETERDING, LJ (reprint author), NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. RI Tomer, Kenneth/E-8018-2013 NR 20 TC 26 Z9 26 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD AUG 21 PY 1991 VL 554 IS 1-2 BP 73 EP 82 DI 10.1016/S0021-9673(01)88438-7 PG 10 WC Chemistry, Analytical SC Chemistry GA GG693 UT WOS:A1991GG69300007 PM 1665498 ER PT J AU DETERDING, LJ PARKER, CE PERKINS, JR MOSELEY, MA JORGENSON, JW TOMER, KB AF DETERDING, LJ PARKER, CE PERKINS, JR MOSELEY, MA JORGENSON, JW TOMER, KB TI NANOSCALE SEPARATIONS - CAPILLARY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY AND CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY FOR THE DETERMINATION OF PEPTIDES AND PROTEINS SO JOURNAL OF CHROMATOGRAPHY LA English DT Article; Proceedings Paper CT 7TH INTERNATIONAL SYMP ON LIQUID CHROMATOGRAPHY-MASS SPECTRUM CY OCT 31-NOV 02, 1990 CL MONTREUX, SWITZERLAND ID FLOW FAST ATOM; ELECTROSPRAY IONIZATION; INNER DIAMETERS; BIOMOLECULES; INTERFACE AB Nanoscale separation techniques, such as packed capillary liquid chromatography and capillary zone electrophoresis in which the column effluent flow-rates are in the nanoliter per minute range rather than microliter per minute range, have been successfully interfaced with continuous-flow fast atom bombardment and electrospray ionization. Applications of these techniques to the separation and determination of peptide mixtures, protein digests and protein mixtures are presented. C1 NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709. UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27514. RI Tomer, Kenneth/E-8018-2013 NR 23 TC 49 Z9 49 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD AUG 21 PY 1991 VL 554 IS 1-2 BP 329 EP 338 DI 10.1016/S0021-9673(01)88460-0 PG 10 WC Chemistry, Analytical SC Chemistry GA GG693 UT WOS:A1991GG69300029 ER PT J AU HAVLIN, S BULDYREV, SV STANLEY, HE WEISS, GH AF HAVLIN, S BULDYREV, SV STANLEY, HE WEISS, GH TI PROBABILITY-DISTRIBUTION OF THE INTERFACE WIDTH IN SURFACE ROUGHENING - ANALOGY WITH A LEVY FLIGHT SO JOURNAL OF PHYSICS A-MATHEMATICAL AND GENERAL LA English DT Letter ID MODEL AB We present a closed-form expression for the probability distribution of the height fluctuations in the Zhang model of anomalous surface roughening. The result-which includes both the steady state behaviour and the time evolution to the steady state-is based on an analogy between the (d = 1 + l)-dimensional 'surface' problem and a d = 1 Levy flight. In the limit case of conventional ballistic deposition we obtain a Gaussian distribution for the height fluctuations. Our results are corroborated by detailed numerical simulations. C1 BOSTON UNIV,DEPT PHYS,BOSTON,MA 02215. NIH,DIV COMP RES & TECHNOL,BETHESDA,MD 20205. RP HAVLIN, S (reprint author), BOSTON UNIV,CTR POLYMER STUDIES,BOSTON,MA 02215, USA. RI Buldyrev, Sergey/I-3933-2015 NR 17 TC 20 Z9 20 U1 0 U2 0 PU IOP PUBLISHING LTD PI BRISTOL PA TECHNO HOUSE, REDCLIFFE WAY, BRISTOL, ENGLAND BS1 6NX SN 0305-4470 J9 J PHYS A-MATH GEN JI J. Phys. A-Math. Gen. PD AUG 21 PY 1991 VL 24 IS 16 BP L925 EP L931 DI 10.1088/0305-4470/24/16/008 PG 7 WC Physics, Multidisciplinary; Physics, Mathematical SC Physics GA GC466 UT WOS:A1991GC46600008 ER PT J AU SHOPLAND, DR EYRE, HJ PECHACEK, TF AF SHOPLAND, DR EYRE, HJ PECHACEK, TF TI SMOKING-ATTRIBUTABLE CANCER MORTALITY IN 1991 - IS LUNG-CANCER NOW THE LEADING CAUSE OF DEATH AMONG SMOKERS IN THE UNITED-STATES SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID CIGARETTE-SMOKING; BRITISH DOCTORS; WOMEN; RISK; MEN AB Findings from the new American Cancer Society prospective study of 1.2 million men and women indicate that mortality risks among smokers have increased substantially for most of the eight major cancer sites causally associated with cigarette smoking. Lung cancer risk for male smokers doubled, while the risk for females increased more than fourfold. On the basis of the new American Cancer Society relative risks, we project that cigarette smoking alone will contribute to slightly more than 157 000 of the 514 000 total cancer deaths expected to occur in the United States in 1991. Overall, smoking directly contributes to 21.5% of all cancer deaths in women but 45% of all cancer deaths in men. It would also appear that lung cancer has now displaced coronary heart disease as the single leading cause of excess mortality among smokers in the United States. RP SHOPLAND, DR (reprint author), NCI,DIV CANC PREVENT & CONTROL,SMOKING & TOBACCO CONTROL PROGRAM,BETHESDA,MD 20892, USA. NR 55 TC 234 Z9 235 U1 0 U2 2 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD AUG 21 PY 1991 VL 83 IS 16 BP 1142 EP 1148 DI 10.1093/jnci/83.16.1142 PG 7 WC Oncology SC Oncology GA GB432 UT WOS:A1991GB43200011 PM 1886147 ER PT J AU EGUCHI, Y TOMIZAWA, J AF EGUCHI, Y TOMIZAWA, J TI COMPLEXES FORMED BY COMPLEMENTARY RNA STEM-LOOPS - THEIR FORMATIONS, STRUCTURES AND INTERACTION WITH COLE1 ROM PROTEIN SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE RNA RNA INTERACTION; RNA-I; RNA-II; COLE1 ROM PROTEIN; RNA STEM-LOOPS ID PLASMID REPLICATION; PRIMER TRANSCRIPT; BINDING; ANTICODONS; INHIBITION; DNA C1 NIDDKD,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 19 TC 96 Z9 96 U1 1 U2 1 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD AUG 20 PY 1991 VL 220 IS 4 BP 831 EP 842 DI 10.1016/0022-2836(91)90356-B PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GC604 UT WOS:A1991GC60400003 PM 1715406 ER PT J AU RIUS, RA STREATY, RA LOH, YP KLEE, WA AF RIUS, RA STREATY, RA LOH, YP KLEE, WA TI DEVELOPMENTAL EXPRESSION OF G-PROTEINS THAT DIFFERENTIALLY MODULATE ADENYLYL CYCLASE ACTIVITY IN MOUSE-BRAIN SO FEBS LETTERS LA English DT Article DE DEVELOPMENT (MOUSE BRAIN); G-PROTEIN; ALTERNATIVE SPLICING; ADENYLYL CYCLASE; OPIATE RECEPTOR ID GTP-BINDING PROTEINS; BETA-ADRENERGIC RECEPTORS; ALPHA-SUBUNIT; RAT-BRAIN; MOLECULAR-CLONING; 2 FORMS; POSTNATAL-DEVELOPMENT; REGULATORY PROTEINS; OPIATE RECEPTORS; IDENTIFICATION AB Changes in the relative abundance of the G protein alpha-subunits were observed during early mouse development G(s-alpha) was almost exclusively present as a large form (G(s-1)) in prenatal brain. Postnatally with a substantial increase in Gpp[NH]p stimulated adenylyl cyclase activity, the small form (G(s-s)) increased in amount while G(s-1) decreased. These results suggest that the G(s-s) may be the more effective cyclase activator and that changes in alternative splicing are developmentally regulated. G(i1) and G(o) appeared before birth whereas G(i2) developed postnatally. Opiate stimulation of GTPase and inhibition of adenylyl cyclase were fully expressed prenatally. C1 NIMH,MOLEC BIOL LAB,BETHESDA,MD 20892. RP RIUS, RA (reprint author), NICHHD,DEV NEUROBIOL LAB,CELLULAR NEUROBIOL SECT,9000 ROCKVILLE PIKE,BLDG 36 2A 21,BETHESDA,MD 20892, USA. NR 44 TC 31 Z9 31 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD AUG 19 PY 1991 VL 288 IS 1-2 BP 51 EP 54 DI 10.1016/0014-5793(91)81001-O PG 4 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA GD736 UT WOS:A1991GD73600014 PM 1652479 ER PT J AU SHU, XO BRINTON, LA ZHENG, W GAO, YT FAN, J FRAUMENI, JF AF SHU, XO BRINTON, LA ZHENG, W GAO, YT FAN, J FRAUMENI, JF TI A POPULATION-BASED CASE-CONTROL STUDY OF ENDOMETRIAL CANCER IN SHANGHAI, CHINA SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID RISK-FACTORS; ORAL-CONTRACEPTIVES; CIGARETTE-SMOKING; ESTROGENS; EPIDEMIOLOGY; PROGESTERONE; PREVENTION; WOMEN AB A case-control study of 268 patients with endometrial cancer and 268 population controls was conducted during 1988-1990 in Shanghai, China, to evaluate etiologic factors in a population whose risk had not been substantially altered by the use of exogenous estrogens. In spite of this, the major risk factors resembled those found in other studies. The risk of endometrial cancer was significantly elevated among nulligravidas (OR = 5.4, 95% Cl = 2.0-14.6) and decreased with number of pregnancies (p < 0.01). Late age at menopause was associated with increased risk, while early age at menarche was unrelated. Use of oral contraceptives for more than 2 years was associated with a reduction in endometrial cancer risk (OR = 0.4, 95% Cl = 0.1-1.2), while short-term use of oral contraceptives and other methods of contraception were unrelated. Obesity was a strong predictor of risk, with women in the highest quartile of weight having 2.5 times the risk of those in the lowest quartile. In contrast to many other studies, cigarette smokers were at elevated risk (OR = 1.7, 95% Cl = 0.9-3.0). Risk was also elevated among women reporting a history of gall-bladder disease, polycystic ovaries, menstrual symptoms, and non-estrogen hormone use. C1 NCI,ENVIRONM EPIDEMIOL BRANCH,EXECUT PLAZA N,RM 443,BETHESDA,MD 20892. NCI,EPIDEMIOL & BIOSTAT PROGRAM,BETHESDA,MD 20892. SHANGHAI CANC INST,DEPT EPIDEMIOL,SHANGHAI,PEOPLES R CHINA. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 NR 26 TC 53 Z9 55 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD AUG 19 PY 1991 VL 49 IS 1 BP 38 EP 43 DI 10.1002/ijc.2910490108 PG 6 WC Oncology SC Oncology GA GB773 UT WOS:A1991GB77300007 PM 1874568 ER PT J AU EVANS, CP WALSH, DS KOHN, EC AF EVANS, CP WALSH, DS KOHN, EC TI AN AUTOCRINE MOTILITY FACTOR SECRETED BY THE DUNNING R-3327 RAT PROSTATIC ADENOCARCINOMA CELL SUBTYPE-AT2.1 SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID HUMAN-MELANOMA CELLS; ADENYLATE-CYCLASE; METASTATIC ABILITY; GRADING SYSTEM; TUMOR-CELLS; CANCER; MODEL; PROTEINS; PREDICTION; CHEMOTAXIS AB Tumor cell locomotion is an integral part of the metastatic process. We present a new autocrine motility factor (AMF) derived from the serum-free conditioned medium of the Dunning R-3327 rat prostate adenocarcinoma AT2.1 tumor cell subline AT2.1-AMF, prepared by concentration of components less-than-or-equal-to 30 kDa in size and washed free of low-molecular-weight growth factors, stimulated motility of AT2.1 cells in modified Boyden chamber migration assays. This stimulated migration was dose-dependent, and by checkerboard analysis was both chemotactic and chemokinetic. AT2.1-AMF activity was labile to heat, acid, base, reduction, oxidation, and proteases. Lyophilization and treatment with 6M urea caused a mild decrease (< 20%) in migration-stimulating capability. Tumor-cell specificity was demonstrated for AMF of AT2.1 and AT3.1 Dunning sublines, and the A2058 human melanoma cell lines. AT2.1 cell migration to AT2.1-AMF was inhibited by 2 hr pre-treatment with chlorea toxin (0.1-mu-g/ml) or forskolin (100-mu-M), but not altered by 2 hr pre-treatment with pertussis toxin (1.0-mu-g/ml). This indicates that guanine nucleotide binding protein-mediated regulation of cAMP is involved in modulating the AT2.1 cell response to its AMF. The AT2.1-AMF belongs to a related family of tumor autocrine motility factors and represents a new model for understanding the role of tumor-cell migration in the metastatic process of human prostate cancer. C1 NCI,MED BRANCH,BLDG 10,ROOM 2A33,BETHESDA,MD 20892. WALTER REED ARMY MED CTR,DIV SURG,WASHINGTON,DC 20307. NR 36 TC 39 Z9 39 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD AUG 19 PY 1991 VL 49 IS 1 BP 109 EP 113 DI 10.1002/ijc.2910490120 PG 5 WC Oncology SC Oncology GA GB773 UT WOS:A1991GB77300019 PM 1874563 ER PT J AU BIRDER, LA ROPPOLO, JR IADAROLA, MJ DEGROAT, WC AF BIRDER, LA ROPPOLO, JR IADAROLA, MJ DEGROAT, WC TI ELECTRICAL-STIMULATION OF VISCERAL AFFERENT PATHWAYS IN THE PELVIC NERVE INCREASES C-FOS IN THE RAT LUMBOSACRAL SPINAL-CORD SO NEUROSCIENCE LETTERS LA English DT Article DE ELECTRICAL STIMULATION; PELVIC AFFERENT; URINARY BLADDER; C-FOS ID EXPRESSION; PROTEIN; NEURONS AB Electrical stimulation (20-35 Hz, 2-5 V, 1.5 h) of the pelvic nerve in urethane-anesthetized rats increased the expression of c-fos protein-immunoreactivity primarily in neurons in the L6-S1 segements of the spinal cord. The neurons were localized to areas receiving afferent input from the pelvic viscera including the superficial dorsal horn, the dorsal commissure, and lateral laminae V-VII in the region of the sacral parasympathetic nucleus. These experiments indicate that (1) electrical stimulation of abdominal nerves following surgical exposure is a useful method for tracing visceral afferent pathways and (2) afferent information from the pelvic viscera is received by neurons in specific areas of the dorsal horn. C1 UNIV PITTSBURGH,SCH MED,DEPT BEHAV NEUROSCI,PITTSBURGH,PA 15261. NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BETHESDA,MD 20892. RP BIRDER, LA (reprint author), UNIV PITTSBURGH,SCH MED,DEPT PHARMACOL,13TH FLOOR BIOMED SCI TOWER,PITTSBURGH,PA 15261, USA. FU NIDDK NIH HHS [DK-42369, DK-37241] NR 14 TC 71 Z9 71 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD AUG 19 PY 1991 VL 129 IS 2 BP 193 EP 196 DI 10.1016/0304-3940(91)90459-7 PG 4 WC Neurosciences SC Neurosciences & Neurology GA GC644 UT WOS:A1991GC64400009 PM 1745400 ER PT J AU MARMOR, M HARTSOCK, P AF MARMOR, M HARTSOCK, P TI SELF-DESTRUCTING (NON-REUSABLE) SYRINGES SO LANCET LA English DT Editorial Material C1 NIDR,ROCKVILLE,MD. RP MARMOR, M (reprint author), NYU,SCH MED,NEW YORK,NY 10003, USA. OI Marmor, Michael/0000-0001-6605-2661 NR 1 TC 7 Z9 7 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD AUG 17 PY 1991 VL 338 IS 8764 BP 438 EP 439 DI 10.1016/0140-6736(91)91054-X PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA GB446 UT WOS:A1991GB44600018 PM 1678097 ER PT J AU RAY, PE RULEY, EJ SAAVEDRA, JM AF RAY, PE RULEY, EJ SAAVEDRA, JM TI DIFFERENT EFFECTS OF CHRONIC K+ DEPLETION ON FOREBRAIN AND PERIPHERAL ANGIOTENSIN-II RECEPTORS IN YOUNG-RATS SO BRAIN RESEARCH LA English DT Article DE BRAIN ANGIOTENSIN-II RECEPTOR; KIDNEY ANGIOTENSIN-II RECEPTOR; ADRENAL ANGIOTENSIN-II RECEPTOR; RENIN ANGIOTENSIN SYSTEM; ION DEFICIENCY; DEVELOPMENT ID SUBFORNICAL ORGAN; BINDING-SITES; AUTORADIOGRAPHIC CHARACTERIZATION; SODIUM DEPLETION; PITUITARY-GLAND; BRAIN NUCLEI; VASOPRESSIN; PLASMA; RELEASE; PASTE AB K+ depletion stimulates the circulating renin-angiotensin system and affects the regulation of peripheral angiotensin II receptors. The effects of K+ depletion on the regulation of central angiotensin II receptors are unknown. We studied the effects of selective K+ depletion (< 0.05% in diet for 16 days) on angiotensin II receptor number in kidneys, adrenal glands, and selected brain areas of young rats. K+ depletion caused a significant increase in plasma renin activity and significantly decreased angiotensin II receptor number in the kidney glomeruli and medulla, and in the adrenal zona glomerulosa and adrenal medulla. In the brain, the angiotensin II receptor number was unchanged in the subfornical organ and the hypothalamic paraventricular nucleus after 16 days of K+ depletion. An additional NaCl supplementation (0.02% in the drinking water) to K+-depleted rats produced a decrease in plasma renin activity but failed to affect subfornical organ or paraventricular angiotensin II receptor number. Our results suggest that in young animals, K+ depletion has a significant impact on the peripheral renin-angiotensin system without affecting the density of forebrain angiotensin II receptors. C1 NIMH,CLIN SCI LAB,PHARMACOL SECT,9000 ROCKVILLE PIKE,BLDG 10,ROOM 2D-45,BETHESDA,MD 20892. CHILDRENS NATL MED CTR,DEPT NEPHROL,WASHINGTON,DC. NR 37 TC 11 Z9 11 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD AUG 16 PY 1991 VL 556 IS 2 BP 240 EP 246 DI 10.1016/0006-8993(91)90311-I PG 7 WC Neurosciences SC Neurosciences & Neurology GA GE893 UT WOS:A1991GE89300006 PM 1933358 ER PT J AU CAVIEDES, P KOISTINAHO, J AULT, B RAPOPORT, SI AF CAVIEDES, P KOISTINAHO, J AULT, B RAPOPORT, SI TI EFFECTS OF NERVE GROWTH-FACTOR ON ELECTRICAL MEMBRANE-PROPERTIES OF CULTURED DORSAL-ROOT GANGLIA NEURONS FROM NORMAL AND TRISOMY-21 HUMAN FETUSES SO BRAIN RESEARCH LA English DT Article DE NERVE GROWTH FACTOR; ACTION POTENTIAL; TISSUE CULTURE; DORSAL ROOT GANGLION; CURRENT CLAMP ID SENSORY NEURONS; SODIUM CURRENTS; DOWNS-SYNDROME; SPINAL-CORD; PC12 CELLS; ANTI-NGF; MOUSE; MODEL; ABNORMALITIES; AXOTOMY AB Trisomy 21 (Down syndrome) results in abnormalities of electrical membrane properties of cultured human fetal dorsal root ganglion (DRG) neurons; namely, faster rates of depolarization and repolarization of the action potential, and a shortened spike duration. A possible role of nerve growth factor (NGF) in the expression of abnormal electrical membrane properties fetal human DRG neurons from trisomy 21 subjects was examined. DRG neurons obtained from normal and trisomy 21 abortuses of 16-20 weeks gestation were cultured in the presence or absence of 40 nM 7S NGF. After 1 week in culture, action potentials were recorded using the whole cell patch-clamp technique, in current clamp mode. At the resting membrane potential, normal (diploid) neurons grown without NGF showed reduced maximal rates of depolarization (-41.3%) and of repolarization (-31.4%), a decreased spike amplitude (-14.2%) and a prolonged action potential (+49.2%), when compared to normal cells cultured with NGF. Trisomy 21 neurons showed similar changes, but had a greater relative decrease in the rates of action potential depolarization and repolarization. These changes were evident at different membrane potentials. Normal and trisomic DRG neurons cultured without NGF showed differences in action potential parameters similar to those previously described using NGF-supplemented culture medium. These data indicate that NGF can regulate electrical membrane properties in cultured human fetal DRG neurons, but apparently is not responsible for the abnormalities observed in trisomy 21 neurons. C1 NIA,NEUROSCI LAB,BETHESDA,MD 20892. NR 37 TC 18 Z9 18 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD AUG 16 PY 1991 VL 556 IS 2 BP 285 EP 291 DI 10.1016/0006-8993(91)90317-O PG 7 WC Neurosciences SC Neurosciences & Neurology GA GE893 UT WOS:A1991GE89300012 PM 1834314 ER PT J AU RUBIN, JR HAROMY, TP SUNDARALINGAM, M AF RUBIN, JR HAROMY, TP SUNDARALINGAM, M TI STRUCTURE OF THE ANTICANCER DRUG COMPLEX TETRAKIS MU-ACETATO)-BIS(1-METHYLADENOSINE)DIRHODIUM(II) MONOHYDRATE SO ACTA CRYSTALLOGRAPHICA SECTION C-CRYSTAL STRUCTURE COMMUNICATIONS LA English DT Note AB [Rh2(C2H3O2)4(C11H16N5O4)2].H2O, M(r) = 1024.6, triclinic, P1, a = 7.808 (3), b = 11.469 (4), c = 12.091 (2) angstrom, alpha = 69.55 (2), beta = 79.46 (2), gamma = 76.61 (3)-degrees, V = 980.7 (6) angstrom 3, Z = 1, D(x) = 1.735 g cm-3, lambda(Cu K-alpha) = 1.5418 angstrom, mu = 77.6 cm-1, F(000) = 522, room temperature, R = 0.053 for 3638 unique reflections. Structure consists of two rhodium(II) ions in a metal-metal bond bridged by four acetate groups. The remaining axial coordination sites on the rhodium ions are coordinated to the N(7) positions of two 1-methyladenosine molecules. C1 NCI,FREDERICK CANC RES FACIL,FREDERICK,MD 21701. UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BIOCHEM,MADISON,WI 53706. FU NIGMS NIH HHS [GM-17378]; PHS HHS [N01-C01-74101] NR 4 TC 52 Z9 52 U1 0 U2 1 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0108-2701 J9 ACTA CRYSTALLOGR C JI Acta Crystallogr. Sect. C-Cryst. Struct. Commun. PD AUG 15 PY 1991 VL 47 BP 1712 EP 1714 DI 10.1107/S010827019100032X PN 8 PG 3 WC Chemistry, Multidisciplinary; Crystallography SC Chemistry; Crystallography GA GC569 UT WOS:A1991GC56900048 PM 1781962 ER PT J AU KINGSLEY, LA ZHOU, SYJ BACELLAR, H RINALDO, CR CHMIEL, J DETELS, R SAAH, A VANRADEN, M HO, M MUNOZ, A AF KINGSLEY, LA ZHOU, SYJ BACELLAR, H RINALDO, CR CHMIEL, J DETELS, R SAAH, A VANRADEN, M HO, M MUNOZ, A TI TEMPORAL TRENDS IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 SEROCONVERSION 1984-1989 - A REPORT FROM THE MULTICENTER AIDS COHORT STUDY (MACS) SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE ACQUIRED IMMUNODEFICIENCY SYNDROME; HIV; HIV SEROPOSITIVITY; HIV SEROPREVALENCE ID HOMOSEXUAL BISEXUAL MEN; INFECTION; HEALTH AB The 5-year temporal trends in human immunodeficiency virus type 1 (HIV-1) seroconversion between 1984 and 1989 among homosexual/bisexual men participating in the Multicenter AIDS Cohort Study (MACS) are reported. Of 3,262 initially seronegative men, 368 (11.3%) had seroconverted by December 31, 1989. Although the incidence of seroconversion declined precipitously during the first 3 years of follow-up (from 4.1% to 0.9% per 6 months), no evidence for a further substantial reduction was noted after mid-1987, since 6-month incidence rates ranged between 0.5% and 1.2%. The Chicago cohort experienced an increase in HIV-1 seroconversion during both semesters of 1989; 2.1% and 1.6% per 6 months, respectively, became newly infected. Other MACS centers did not report such an increase. Center-specific differences were observed by race; black men were at higher seroconversion risk than white men in Baltimore/Washington (relative risk (RR) = 3.4) and Chicago (RR = 2.4), while Hispanic men were at higher risk than white men in Chicago (RR = 3.3). Younger age (< 35 years) was also associated with HIV-1 seroconversion (RR = 1.5). It is disturbing to report an overall annual seroconversion rate of 1.2% for the 2 years prior to December 31, 1989, as well as evidence for a sustained recent increase in Chicago during 1989. Long-term maintenance of safe-sex behaviors should be the cornerstone of acquired immunodeficiency syndrome prevention among homosexual/bisexual men. C1 UNIV PITTSBURGH,GRAD SCH PUBL HLTH,DEPT EPIDEMIOL,PITTSBURGH,PA 15261. JOHNS HOPKINS UNIV,SCH PUBL HLTH,DEPT EPIDEMIOL,BALTIMORE,MD 21218. NORTHWESTERN UNIV,SCH MED,CTR CANC,BIOMETRY SECT,CHICAGO,IL 60611. UNIV CALIF LOS ANGELES,SCH PUBL HLTH,DEPT EPIDEMIOL,LOS ANGELES,CA 90024. NIAID,BETHESDA,MD 20892. RP KINGSLEY, LA (reprint author), UNIV PITTSBURGH,GRAD SCH PUBL HLTH,DEPT INFECT DIS & MICROBIOL,130 DESOTO ST,A417 CRABTREE HALL,PITTSBURGH,PA 15261, USA. NR 18 TC 78 Z9 79 U1 0 U2 0 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD AUG 15 PY 1991 VL 134 IS 4 BP 331 EP 339 PG 9 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GD317 UT WOS:A1991GD31700001 PM 1877593 ER PT J AU BROWN, LM DOSEMECI, M BLAIR, A BURMEISTER, L AF BROWN, LM DOSEMECI, M BLAIR, A BURMEISTER, L TI COMPARABILITY OF DATA OBTAINED FROM FARMERS AND SURROGATE RESPONDENTS ON USE OF AGRICULTURAL PESTICIDES SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE AGRICULTURE; EPIDEMIOLOGIC METHODS; INTERVIEWS; PESTICIDES; QUESTIONNAIRES ID NEXT-OF-KIN; INFORMATION; SPOUSE; RELIABILITY; VALIDITY; EXPOSURE; ACCURACY; SMOKING AB Information from surrogates is increasingly being used in case-control studies to evaluate cancer risks from pesticides; however, little is known about the quality of this type of information. To address this concern, the authors compared interview data collected in 1987 from 95 male Iowa farmers and their wives or other surrogates on the use of specific agricultural pesticides. Agreement between direct and surrogate interviews was excellent (83-100%) for responses to dichotomous (yes/no) questions regarding past agricultural use of specific pesticides. Although there were more discrepancies for detailed questions (e.g., the number of days per year on which each pesticide was handled), responses from spouses appear to be adequate for epidemiologic studies of pesticides and cancer. C1 UNIV IOWA,DEPT PREVENT MED,IOWA CITY,IA 52242. RP BROWN, LM (reprint author), NCI,EPIDEMIOL & BIOSTAT PROGRAM,EXECUT PLAZA N,ROOM 415C,BETHESDA,MD 20892, USA. NR 15 TC 39 Z9 39 U1 0 U2 1 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD AUG 15 PY 1991 VL 134 IS 4 BP 348 EP 355 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GD317 UT WOS:A1991GD31700003 PM 1877595 ER PT J AU CANNER, PL BORHANI, NO OBERMAN, A CUTLER, J PRINEAS, RJ LANGFORD, H HOOPER, FJ AF CANNER, PL BORHANI, NO OBERMAN, A CUTLER, J PRINEAS, RJ LANGFORD, H HOOPER, FJ TI THE HYPERTENSION PREVENTION TRIAL - ASSESSMENT OF THE QUALITY OF BLOOD-PRESSURE MEASUREMENTS SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE BLOOD PRESSURE DETERMINATION; CLINICAL TRIALS; EPIDEMIOLOGIC METHODS; HYPERTENSION; QUALITY CONTROL ID WITHIN-PERSON VARIABILITY AB Blood pressure variability is an important consideration in hypertension trials for determining required sample size and consequently making accurate outcome statements. The Hypertension Prevention Trial was a randomized controlled trial carried out in 1983-1986 in four US clinics on men and women with high normal diastolic blood pressure. This trial provided an opportunity to assess conditions affecting blood pressure measurement variability. Trained blood pressure observers measured systolic and diastolic blood pressures twice, 30 seconds apart, using a random-zero sphygmomanometer. The quality of blood pressure measurements was assessed by computing the variability of the two readings per participant-visit for each blood pressure observer at each study clinic. Other sources of variability investigated included observer digit preference, time of day, and ambient temperature. On the basis of data from this population, it is estimated that the standard deviation of blood pressure values can be reduced by 5% by taking two measurements per participant-visit. An additional reduction of variability can be effected by having the duplicate blood pressure measurements made by different blood pressure observers. In special instances where the range of blood pressure values is very restricted, use of the random-zero sphygmomanometer can increase or decrease the among-participant variability in blood pressure values, depending upon where the distribution of blood pressure values is centered. C1 MARYLAND MED RES INST,BALTIMORE,MD. UNIV CALIF DAVIS,DEPT COMMUNITY HLTH,DAVIS,CA 95616. UNIV ALABAMA,DEPT PREVENT MED,BIRMINGHAM,AL 35294. NHLBI,DIV EPIDEMIOL & CLIN APPLICAT,BETHESDA,MD 20892. UNIV MINNESOTA,SCH PUBL HLTH,DIV EPIDEMIOL,MINNEAPOLIS,MN 55455. UNIV MISSISSIPPI,DEPT MED,JACKSON,MS 39216. FU NHLBI NIH HHS [R01 HL25192, R01 HL25194, R01 HL25201] NR 22 TC 58 Z9 58 U1 0 U2 2 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD AUG 15 PY 1991 VL 134 IS 4 BP 379 EP 392 PG 14 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GD317 UT WOS:A1991GD31700007 PM 1877599 ER PT J AU WEINBERG, CR SANDLER, DP AF WEINBERG, CR SANDLER, DP TI RANDOMIZED RECRUITMENT IN CASE-CONTROL STUDIES SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE BIAS(EPIDEMIOLOGY); CASE-CONTROL STUDIES; EPIDEMIOLOGIC METHODS; INTERACTION; MATCHING; RANDOMIZATION; STUDY DESIGN ID LOGISTIC-REGRESSION; EXPOSURE; 2-STAGE; DISEASE; DESIGN; RISK AB A new sampling approach for case-control studies offers a flexible alternative to frequency matching. In the "randomized recruitment" method, subjects are individually randomized to be recruited or not on the basis of investigator-imposed recruitment probabilities that can depend on both disease status and values of covariates already available or ascertained in a screening interview. When there is prior information about the odds ratios associated with the screening variables, such a design can achieve "probability matching," without the well-known disadvantages that encumber traditional matching. The method can also be used to enlarge the relative size of subsamples of interest. Following randomized recruitment, a modified logistic regression analysis allows unbiased estimation of effects associated with all variables studied, including the "matching" variables. One can also readily fit an additive model. The method is illustrated by developing the recruitment probabilities required for probability matching on age, sex, and cigarette smoking status in an ongoing study of lung cancer and exposure to radon progeny in which smoking cases are undersampled. C1 NIEHS,DIV BIOMETRY & RISK ASSESSMENT,EPIDEMIOL BRANCH,RES TRIANGLE PK,NC 27709. RP WEINBERG, CR (reprint author), NIEHS,DIV BIOMETRY & RISK ASSESSMENT,STAT & BIOMATH BRANCH,POB 12233,RES TRIANGLE PK,NC 27709, USA. OI Sandler, Dale/0000-0002-6776-0018 NR 12 TC 137 Z9 138 U1 0 U2 2 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD AUG 15 PY 1991 VL 134 IS 4 BP 421 EP 432 PG 12 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GD317 UT WOS:A1991GD31700011 PM 1877602 ER PT J AU WACHOLDER, S DOSEMECI, M LUBIN, JH AF WACHOLDER, S DOSEMECI, M LUBIN, JH TI BLIND ASSIGNMENT OF EXPOSURE DOES NOT ALWAYS PREVENT DIFFERENTIAL MISCLASSIFICATION SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE BIAS (EPIDEMIOLOGY); CASE-CONTROL STUDIES; COHORT STUDIES; EPIDEMIOLOGIC METHODS; MISCLASSIFICATION, NONDIFFERENTIAL AB The authors argue that one can never be certain whether an exposure variable which is measured with error is subject to differential misclassification in either a case-control study or a cohort study. They present hypothetic examples that demonstrate that even when misclassification is nondifferential in a 2 x 3 table, the observed odds ratios in the 2 x 2 table created by collapsing over two exposure levels can be either in the opposite direction from or more extreme than the odds ratio that would be obtained if exposures were classified correctly. The anomalies are explained by the observation that the 2 x 2 tables exhibit differential misclassification. In general, collapsing over categories which have different risks of disease and different probabilities of exposure misclassification can induce differential misclassification and even nonconservative estimates of relative risk. Collapsing of exposure levels can occur in the analysis or at the exposure assessment stage. Since indistinguishable categories can be collapsed implicitly, blind assessment of exposure, i.e., assignment without knowledge of disease status, does not guarantee that misclassification is nondifferential. RP WACHOLDER, S (reprint author), NCI,EPIDEMIOL & BIOSTAT PROGRAM,EXECUT PLAZA N,ROOM 403,6130 EXECUT BLVD,ROCKVILLE,MD 20892, USA. NR 3 TC 45 Z9 45 U1 1 U2 3 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD AUG 15 PY 1991 VL 134 IS 4 BP 433 EP 437 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GD317 UT WOS:A1991GD31700012 PM 1877603 ER PT J AU DOSEMECI, M WACHOLDER, S LUBIN, JH AF DOSEMECI, M WACHOLDER, S LUBIN, JH TI DOES NONDIFFERENTIAL MISCLASSIFICATION OF EXPOSURE ALWAYS BIAS A TRUE EFFECT TOWARD THE NULL VALUE - REPLY SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Letter RP DOSEMECI, M (reprint author), NCI,EPIDEMIOL & BIOSTAT PROGRAM,BETHESDA,MD 20892, USA. NR 5 TC 7 Z9 7 U1 0 U2 1 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD AUG 15 PY 1991 VL 134 IS 4 BP 441 EP 442 PG 2 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GD317 UT WOS:A1991GD31700016 ER PT J AU NUSSENBLATT, RB PALESTINE, AG CHAN, CC STEVENS, G MELLOW, SD GREEN, SB AF NUSSENBLATT, RB PALESTINE, AG CHAN, CC STEVENS, G MELLOW, SD GREEN, SB TI RANDOMIZED, DOUBLE-MASKED STUDY OF CYCLOSPORINE COMPARED TO PREDNISOLONE IN THE TREATMENT OF ENDOGENOUS UVEITIS SO AMERICAN JOURNAL OF OPHTHALMOLOGY LA English DT Article ID POSTERIOR UVEITIS; DISEASE; NEPHROTOXICITY; THERAPY AB Fifty-six patients with bilateral sight-threatening noninfectious intermediate or posterior uveitis participated in a randomized double-masked study of the use of cyclosporine vs prednisolone in their treatment. Applying the end-point definitions, visual acuity or vitreal haze improved in only 13 of 28 (46%) patients in each group. The macular edema resolved in seven of 15 patients of the cyclosporine-treated group, and in ten of 16 patients of the prednisolone-treated group (P = .376). Patients whose therapies failed both cyclosporine and prednisolone trials were treated with both drugs, which resulted in additional patient improvements. Secondary effects were observed in both therapeutic alternatives, the most notable being alterations in serum creatinine concentration and hypertension with the dosage of cyclosporine used. C1 NCI,CLIN & DIAGNOST TRIALS CLIN,BETHESDA,MD 20892. RP NUSSENBLATT, RB (reprint author), NEI,IMMUNOL LAB,BLDG 10,RM 10N202,BETHESDA,MD 20892, USA. NR 16 TC 109 Z9 113 U1 0 U2 4 PU OPHTHALMIC PUBL CO PI CHICAGO PA 77 WEST WACKER DR, STE 660, CHICAGO, IL 60601 SN 0002-9394 J9 AM J OPHTHALMOL JI Am. J. Ophthalmol. PD AUG 15 PY 1991 VL 112 IS 2 BP 138 EP 146 PG 9 WC Ophthalmology SC Ophthalmology GA FZ986 UT WOS:A1991FZ98600003 PM 1867297 ER PT J AU NUSSENBLATT, RB PALESTINE, AG AF NUSSENBLATT, RB PALESTINE, AG TI HUMAN-IMMUNODEFICIENCY-VIRUS, HERPES-ZOSTER, AND THE RETINA SO AMERICAN JOURNAL OF OPHTHALMOLOGY LA English DT Editorial Material RP NUSSENBLATT, RB (reprint author), NEI,BLDG 10,RM 10N202,BETHESDA,MD 20892, USA. NR 3 TC 3 Z9 4 U1 0 U2 0 PU OPHTHALMIC PUBL CO PI CHICAGO PA 77 WEST WACKER DR, STE 660, CHICAGO, IL 60601 SN 0002-9394 J9 AM J OPHTHALMOL JI Am. J. Ophthalmol. PD AUG 15 PY 1991 VL 112 IS 2 BP 206 EP 207 PG 2 WC Ophthalmology SC Ophthalmology GA FZ986 UT WOS:A1991FZ98600014 PM 1867307 ER PT J AU BOVILL, EG TERRIN, ML STUMP, DC BERKE, AD FREDERICK, M COLLEN, D FEIT, F GORE, JM HILLIS, LD LAMBREW, CT LEIBOFF, R MANN, KG MARKIS, JE PRATT, CM SHARKEY, SW SOPKO, G TRACY, RP CHESEBRO, JH AF BOVILL, EG TERRIN, ML STUMP, DC BERKE, AD FREDERICK, M COLLEN, D FEIT, F GORE, JM HILLIS, LD LAMBREW, CT LEIBOFF, R MANN, KG MARKIS, JE PRATT, CM SHARKEY, SW SOPKO, G TRACY, RP CHESEBRO, JH TI HEMORRHAGIC EVENTS DURING THERAPY WITH RECOMBINANT TISSUE-TYPE PLASMINOGEN-ACTIVATOR, HEPARIN, AND ASPIRIN FOR ACUTE MYOCARDIAL-INFARCTION - RESULTS OF THE THROMBOLYSIS IN MYOCARDIAL-INFARCTION (TIMI), PHASE-II TRIAL SO ANNALS OF INTERNAL MEDICINE LA English DT Article ID LEFT-VENTRICULAR FUNCTION; INTRAVENOUS STREPTOKINASE; REPERFUSION; SURVIVAL AB Objectives: To assess the effects of invasive procedures, hemostatic and clinical variables, the timing of beta-blocker therapy, and the doses of recombinant plasminogen activator (rt-PA) on hemorrhagic events. Design: A multicenter, randomized, controlled trial. Setting: Hospitals participating in the Thrombolysis in Myocardial Infarction, Phase II trial (TIMI II). Interventions: Patients received rt-PA, heparin, and aspirin. The total dose of rt-PA was 150 mg for the first 520 patients and 100 mg for the remaining 2819 patients. Patients were randomly assigned to an invasive strategy (coronary arteriography with percutaneous angioplasty [if feasible] done routinely 18 to 48 hours after the start of thrombolytic therapy) or to a conservative strategy (coronary arteriography done for recurrent spontaneous or exercise-induced ischemia). Eligible patients were also randomly assigned to either immediate intravenous or deferred beta-blocker therapy. Measurements: Patients were monitored for hemorrhagic events during hospitalization. Main Results: In patients on the 100-mg rt-PA regimen, major and minor hemorrhagic events were more common among those assigned to the invasive than among those assigned to the conservative strategy (18.5% versus 12.8%, P < 0.001). Major or minor hemorrhagic events were associated with the extent of fibrinogen breakdown, peak rt-PA levels, thrombocytopenia, prolongation of the activated partial thromboplastin time (APTT) to more than 90 seconds, weight of 70 kg or less, female gender, and physical signs of cardiac decompensation. Immediate intravenous beta-blocker therapy had no important effect on hemorrhagic events when compared with delayed beta-blocker therapy. Intracranial hemorrhages were more frequent among patients treated with the 150-mg rt-PA dose than with the 100-mg rt-PA dose (2.1% versus 0.5%, P < 0.001). The extent of the plasmin-mediated hemostatic defect was also greater in patients receiving the 150-mg dose. Conclusions: Increased morbidity due to hemorrhagic complications is associated with an invasive management strategy in patients with acute myocardial infarction. Our findings show the complex interaction of several factors in the occurrence of hemorrhagic events during thrombolytic therapy. C1 UNIV VERMONT,BURLINGTON,VT 05405. COLUMBIA UNIV,NEW YORK,NY 10027. NYU,NEW YORK,NY 10003. UNIV MASSACHUSETTS,SCH MED,WORCESTER,MA 01605. UNIV TEXAS,SW MED CTR,DALLAS,TX 75230. MAINE MED CTR,PORTLAND,ME 04102. GEORGE WASHINGTON UNIV,WASHINGTON,DC 20052. HARVARD UNIV,SCH MED,BOSTON,MA 02115. BAYLOR COLL MED,HOUSTON,TX 77030. UNIV MINNESOTA,MINNEAPOLIS,MN 55455. NHLBI,BETHESDA,MD 20892. MAYO CLIN & MAYO FDN,ROCHESTER,MN 55905. RP BOVILL, EG (reprint author), MARYLAND MED RES INST,TIMI COORDINATING CTR,600 WYNDHURST AVE,BALTIMORE,MD 21210, USA. FU NHLBI NIH HHS [HL35058, HL38460] NR 37 TC 312 Z9 322 U1 0 U2 3 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD AUG 15 PY 1991 VL 115 IS 4 BP 256 EP 265 PG 10 WC Medicine, General & Internal SC General & Internal Medicine GA GA764 UT WOS:A1991GA76400003 PM 1906692 ER PT J AU MITCHELL, JB DEGRAFF, W KAUFMAN, D KRISHNA, MC SAMUNI, A FINKELSTEIN, E AHN, MS HAHN, SM GAMSON, J RUSSO, A AF MITCHELL, JB DEGRAFF, W KAUFMAN, D KRISHNA, MC SAMUNI, A FINKELSTEIN, E AHN, MS HAHN, SM GAMSON, J RUSSO, A TI INHIBITION OF OXYGEN-DEPENDENT RADIATION-INDUCED DAMAGE BY THE NITROXIDE SUPEROXIDE-DISMUTASE MIMIC, TEMPOL SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article ID MAMMALIAN-CELLS; SPIN LABELS; FREE-RADICALS; POLYFUNCTIONAL RADIOSENSITIZERS; GLUTATHIONE; RADIOPROTECTION; HYDROXYLAMINES; ENHANCEMENT; CYSTEAMINE; RESONANCE C1 HEBREW UNIV JERUSALEM,SCH MED,IL-91010 JERUSALEM,ISRAEL. ELIZABETH GEN HOSP,DEPT RADIAT ONCOL,ELIZABETH,NJ 07202. RP MITCHELL, JB (reprint author), NCI,CLIN ONCOL PROGRAM,RADIAT ONCOL BRANCH,RADIOBIOL SECT,BLDG 10,ROOM B3-B69,BETHESDA,MD 20892, USA. NR 50 TC 194 Z9 197 U1 2 U2 6 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD AUG 15 PY 1991 VL 289 IS 1 BP 62 EP 70 DI 10.1016/0003-9861(91)90442-L PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FX245 UT WOS:A1991FX24500010 PM 1654848 ER PT J AU DEGRAY, JA RAO, DNR MASON, RP AF DEGRAY, JA RAO, DNR MASON, RP TI REDUCTION OF PARAQUAT AND RELATED BIPYRIDYLIUM COMPOUNDS TO FREE-RADICAL METABOLITES BY RAT HEPATOCYTES SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article ID ELECTRON-SPIN-RESONANCE; CENTERED FREE-RADICALS; RED-BLOOD-CELLS; ESCHERICHIA-COLI; HYDROXYL RADICALS; ENDOTHELIAL-CELLS; LIVER; ADRIAMYCIN; MECHANISM; CATION RP DEGRAY, JA (reprint author), NIEHS,MOLEC BIOPHYS LAB,MD 4-01,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 49 TC 33 Z9 33 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD AUG 15 PY 1991 VL 289 IS 1 BP 145 EP 152 DI 10.1016/0003-9861(91)90454-Q PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FX245 UT WOS:A1991FX24500022 PM 1654843 ER PT J AU MOTTLEY, C ROBINSON, RE MASON, RP AF MOTTLEY, C ROBINSON, RE MASON, RP TI FREE-RADICAL FORMATION IN THE OXIDATION OF MALONDIALDEHYDE AND ACETYLACETONE BY PEROXIDASE ENZYMES SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article ID HORSERADISH-PEROXIDASE; MALONALDEHYDE CONTENT; CATALYZED OXIDATION; SINGLET OXYGEN; MUTAGENICITY C1 NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709. LUTHER COLL,DEPT CHEM,DECORAH,IA 52101. NR 31 TC 17 Z9 17 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD AUG 15 PY 1991 VL 289 IS 1 BP 153 EP 160 DI 10.1016/0003-9861(91)90455-R PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FX245 UT WOS:A1991FX24500023 PM 1654844 ER PT J AU DAVIS, BH RAPP, UR DAVIDSON, NO AF DAVIS, BH RAPP, UR DAVIDSON, NO TI RETINOIC ACID AND TRANSFORMING GROWTH-FACTOR-BETA DIFFERENTIALLY INHIBIT PLATELET-DERIVED-GROWTH-FACTOR-INDUCED ITO-CELL ACTIVATION SO BIOCHEMICAL JOURNAL LA English DT Article ID FAT-STORING CELLS; FACTOR RECEPTOR; SIGNAL TRANSDUCTION; LIVER-REGENERATION; RAT; PROLIFERATION; COLLAGEN; EXPRESSION; CULTURE; BROMODEOXYURIDINE AB Sinusoidal Ito cells (stellate or fat-storing cells) undergo excessive cellular proliferation before the establishment and progression of hepatic fibrosis and cirrhosis. Retinoic acid and transforming growth factor-beta (TGF-beta) both inhibit Ito-cell [H-3]thymidine incorporation in serum-containing media. Serum-induced mitogenicity was dependent on platelet-derived growth factor (PDGF). Additionally, pre-treatment of Ito cells with retinoic acid and TGF-beta blocked PDGF-induced cell proliferation. TGF-beta, but not retinoic acid, diminished PDGF-receptor and smooth-muscle alpha-actin abundance. C1 NCI,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21701. RP DAVIS, BH (reprint author), UNIV CHICAGO,DEPT MED,GASTROENTEROL SECT,CHICAGO,IL 60637, USA. FU NHLBI NIH HHS [HL 38180, KO4 HL02166]; NIDDK NIH HHS [DK40223] NR 34 TC 59 Z9 60 U1 0 U2 0 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD AUG 15 PY 1991 VL 278 BP 43 EP 47 PN 1 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GC808 UT WOS:A1991GC80800005 PM 1652942 ER PT J AU ALEXANDER, RC KARP, BI THOMPSON, S KHOT, V KIRCH, DG AF ALEXANDER, RC KARP, BI THOMPSON, S KHOT, V KIRCH, DG TI A DOUBLE-BLIND, PLACEBO-CONTROLLED TRIAL OF DEMECLOCYCLINE TREATMENT OF POLYDIPSIA-HYPONATREMIA IN CHRONICALLY PSYCHOTIC-PATIENTS SO BIOLOGICAL PSYCHIATRY LA English DT Note ID INDUCED WATER-INTOXICATION; DIURNAL WEIGHT-GAIN; SECRETION; LITHIUM; HORMONE C1 NIMH,CTR NEUROSCI,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. NIMH,CTR NEUROSCI,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. COLUMBIA UNIV,SCH PUBL HLTH,DIV BIOSTAT,NEW YORK,NY 10027. NR 14 TC 28 Z9 28 U1 2 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD AUG 15 PY 1991 VL 30 IS 4 BP 417 EP 420 DI 10.1016/0006-3223(91)90300-B PG 4 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA GA214 UT WOS:A1991GA21400014 PM 1912134 ER PT J AU YARCHOAN, R PLUDA, JM PERNO, CF MITSUYA, H BRODER, S AF YARCHOAN, R PLUDA, JM PERNO, CF MITSUYA, H BRODER, S TI ANTIRETROVIRAL THERAPY OF HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION - CURRENT STRATEGIES AND CHALLENGES FOR THE FUTURE SO BLOOD LA English DT Editorial Material ID AIDS-RELATED COMPLEX; IMMUNE-DEFICIENCY SYNDROME; COLONY-STIMULATING FACTOR; RECOMBINANT SOLUBLE CD4; LYMPHADENOPATHY-ASSOCIATED VIRUS; PLACEBO-CONTROLLED TRIAL; III/LYMPHADENOPATHY-ASSOCIATED VIRUS; HIV-1 REPLICATION INVITRO; CELL LYMPHOTROPIC VIRUS; U937 MONOCYTOID CELLS RP YARCHOAN, R (reprint author), NCI,CLIN ONCOL PROGRAM,BLDG 10,RM 13N248,BETHESDA,MD 20892, USA. RI perno, carlo federico/O-1544-2016 NR 251 TC 68 Z9 68 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD AUG 15 PY 1991 VL 78 IS 4 BP 859 EP 884 PG 26 WC Hematology SC Hematology GA GB208 UT WOS:A1991GB20800001 PM 1714326 ER PT J AU MERIGAN, TC AMATO, DA BALSLEY, J POWER, M PRICE, WA BENOIT, S PEREZMICHAEL, A BROWNSTEIN, A KRAMER, AS BRETTLER, D ALEDORT, L RAGNI, MV ANDES, WA GILL, JC GOLDSMITH, J STABLER, S SANDERS, N GJERSET, G LUSHER, J AF MERIGAN, TC AMATO, DA BALSLEY, J POWER, M PRICE, WA BENOIT, S PEREZMICHAEL, A BROWNSTEIN, A KRAMER, AS BRETTLER, D ALEDORT, L RAGNI, MV ANDES, WA GILL, JC GOLDSMITH, J STABLER, S SANDERS, N GJERSET, G LUSHER, J TI PLACEBO-CONTROLLED TRIAL TO EVALUATE ZIDOVUDINE IN TREATMENT OF HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION IN ASYMPTOMATIC PATIENTS WITH HEMOPHILIA SO BLOOD LA English DT Article C1 HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. NIAID,AIDS PROGRAM,BETHESDA,MD 20892. RES TRIANGLE INST,RES TRIANGLE PK,NC 27709. NATL HEMOPHILIA FDN,NEW YORK,NY. MED CTR CENT MASSACHUSETTS MEM,WOOSTER,MA. MT SINAI MED CTR,NEW YORK,NY 10029. UNIV PITTSBURGH,PITTSBURGH,PA 15260. TULANE UNIV,SCH MED,NEW ORLEANS,LA 70112. GREAT LAKES HEMOPHILIA FDN,MILWAUKEE,WI. UNIV NEBRASKA,MED CTR,OMAHA,NE 68105. HUNTINGTON MEM HOSP,CTR HEMOPHILIA,PASADENA,CA 91105. UNIV WASHINGTON,SEATTLE,WA 98195. RP MERIGAN, TC (reprint author), STANFORD UNIV,MED CTR,SCH MED,CTR AIDS RES,RM S-156,GRANT BLDG,STANFORD,CA 94305, USA. FU NIAID NIH HHS [AI-82678, AI-95030] NR 10 TC 48 Z9 48 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD AUG 15 PY 1991 VL 78 IS 4 BP 900 EP 906 PG 7 WC Hematology SC Hematology GA GB208 UT WOS:A1991GB20800005 PM 1831059 ER PT J AU BODINE, DM CROSIER, PS CLARK, SC AF BODINE, DM CROSIER, PS CLARK, SC TI EFFECTS OF HEMATOPOIETIC GROWTH-FACTORS ON THE SURVIVAL OF PRIMITIVE STEM-CELLS IN LIQUID SUSPENSION-CULTURE SO BLOOD LA English DT Article ID COLONY-STIMULATING FACTOR; FORMING CELLS; FACTOR-I; INTERLEUKIN-3-DEPENDENT PROLIFERATION; PROGENITOR CELLS; MOUSE; DIFFERENTIATION; INVITRO; COMBINATION; ENHANCE C1 GENET INST,CAMBRIDGE,MA. RP BODINE, DM (reprint author), NHLBI,CLIN HEMATOL BRANCH,BLDG 10,RM 7C103,BETHESDA,MD 20892, USA. NR 34 TC 107 Z9 107 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD AUG 15 PY 1991 VL 78 IS 4 BP 914 EP 920 PG 7 WC Hematology SC Hematology GA GB208 UT WOS:A1991GB20800007 PM 1714328 ER PT J AU GREINER, JW GUADAGNI, F GOLDSTEIN, D BORDEN, EC RITTS, RE WITT, P LOBUGLIO, AF SALEH, MN SCHLOM, J AF GREINER, JW GUADAGNI, F GOLDSTEIN, D BORDEN, EC RITTS, RE WITT, P LOBUGLIO, AF SALEH, MN SCHLOM, J TI EVIDENCE FOR THE ELEVATION OF SERUM CARCINOEMBRYONIC ANTIGEN AND TUMOR-ASSOCIATED GLYCOPROTEIN-72 LEVELS IN PATIENTS ADMINISTERED INTERFERONS SO CANCER RESEARCH LA English DT Article ID MONOCLONAL-ANTIBODY B72.3; GAMMA-INTERFERON; CA-72-4 RADIOIMMUNOASSAY; COLORECTAL-CANCER; COLON CANCER; TAG-72; CELLS; REACTIVITY; EXPRESSION; ADENOCARCINOMAS AB Sera were collected from 111 patients diagnosed with adenocarcinoma or nonadenocarcinoma malignancies who received different schedules of interferon (IFN)-gamma or IFN-beta-ser alone or in combination. Serum carcinoembryonic antigen (CEA) and tumor-associated glycoprotein-72 (TAG-72) antigen levels were measured to determine whether interferon could enhance the tumor shedding and, thereby, the serum level of either tumor antigen. Less than 10% of the sera samples from patients diagnosed with nonadenocarcinoma malignancies (eg., hairy cell leukemia, melanoma) had positive titers of TAG-72 or CEA, and interferon neither increased nor resulted in the appearance of either tumor antigen in those sera. In contrast, 59.2% and 75.4% of the patients with adenocarcinoma had positive serum levels of TAG-72 and CEA, respectively, prior to interferon. IFN-gamma and IFN-beta-ser alone or in combination significantly increased serum TAG-72 or CEA in approximately 65% of those patients. The results suggest that interferon administration to patients with adenocarcinoma can result in increased serum levels of selected tumor-associated antigens used in the diagnosis of malignancy. These preliminary findings may be important in the development of new strategies to obtain more sensitive tumor antigen serum assays for the diagnosis and monitoring for disease progression of adenocarcinoma. C1 UNIV WISCONSIN,MADISON,WI 53792. MAYO CLIN & MAYO FDN,ROCHESTER,MN 55905. UNIV ALABAMA,BIRMINGHAM,AL 35294. RP GREINER, JW (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,BETHESDA,MD 20892, USA. RI Guadagni, Fiorella/J-4432-2013 OI Guadagni, Fiorella/0000-0003-3652-0457 NR 36 TC 32 Z9 32 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD AUG 15 PY 1991 VL 51 IS 16 BP 4155 EP 4163 PG 9 WC Oncology SC Oncology GA GA543 UT WOS:A1991GA54300007 PM 1907881 ER PT J AU KRUSZEWSKI, FH HENNINGS, H TUCKER, RW YUSPA, SH AF KRUSZEWSKI, FH HENNINGS, H TUCKER, RW YUSPA, SH TI DIFFERENCES IN THE REGULATION OF INTRACELLULAR CALCIUM IN NORMAL AND NEOPLASTIC KERATINOCYTES ARE NOT CAUSED BY RAS GENE-MUTATIONS SO CANCER RESEARCH LA English DT Article ID MOUSE EPIDERMAL-CELLS; CULTURED HUMAN KERATINOCYTES; BOVINE PARATHYROID CELLS; GROWTH-FACTOR RECEPTOR; TERMINAL DIFFERENTIATION; ONCOGENE; CA-2+; CARCINOGENESIS; EXPRESSION; RESISTANT AB The development of resistance to terminal differentiation is an early event in epidermal neoplasia. Altered differentiation can be detected in vitro since normal epidermal cells are induced to differentiate in medium with Ca2+ > 0.1 mM while neoplastic epidermal cells and keratinocytes transduced with a v-ras(Ha) gene are resistant to Ca2+. In normal epidermal cells, the elevation of extracellular Ca2+ (Ca(o)) from 0.05 to 1.2 mM causes a biphasic intracellular Ca2+ (Ca(i)) response in which a transient (10 min) peak of 4-5-fold over basal values is followed by a sustained (> 24 h) 2-fold increase in steady-state Ca(i). The transient peak in Ca(i) is dependent on a serum component and independent of Ca(o), while the sustained plateau is directly dependent on Ca(o). The transient peak responding to a serum factor is lost in normal cells after 24 h in 1.2 mM Ca2+, a time when these cells are differentiating. Two neoplastic keratinocyte cell lines, SP-1 and 308, which produce benign tumors in vivo, also have a biphasic Ca(i) response to an increase in Ca(o). In these cells, the transient peak is also serum dependent and amplified to 10-fold over basal values. However, the plateau value is not sustained and returns to basal values by 8 h, independent of Ca(o). Furthermore, 308 cells remain sensitive to the serum-induced Cal transient after 24 h in 1.2 mM Ca2+. To determine whether the activating c-ras(Ha) mutation in 308 and SP-1 cells was responsible for the altered Ca(i) regulation, a v-ras(Ha) gene was introduced into normal keratinocytes by a defective retrovirus. This also produces the papilloma phenotype in vivo. Recipient cells were resistant to Ca2+-induced terminal differentiation although they did not proliferate in 1.2 mM Ca2+. The Ca(i) profile in response to 1.2 mM Ca2+ was identical in normal and v-ras(Ha) keratinocytes, and these cells lost the serum-induced transient Ca(i) peak after 24 h. Thus, the activation of the c-ras(Ha) gene in 308 or SP-1 cells is probably not solely responsible for the altered Cal response in neoplastic cell lines. Sustained physiological elevation of Ca(i) may be relevant to the loss of proliferative potential in both normal and v-ras(Ha) keratinocytes in 1.2 mM Ca2+. In addition, v-ras(Ha)-mediated or activated c-ras(Ha)-mediated changes in a complementary pathway may contribute to the block in terminal differentiation in neoplastic cells. C1 NCI,DIV CANC ETIOL,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892. JOHNS HOPKINS UNIV HOSP,CTR ONCOL,BALTIMORE,MD 21205. NR 46 TC 24 Z9 24 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD AUG 15 PY 1991 VL 51 IS 16 BP 4206 EP 4212 PG 7 WC Oncology SC Oncology GA GA543 UT WOS:A1991GA54300015 PM 1907882 ER PT J AU DEFRANCISCIS, V ROSATI, R COLUCCIDAMATO, GL EVA, A VECCHIO, G AF DEFRANCISCIS, V ROSATI, R COLUCCIDAMATO, GL EVA, A VECCHIO, G TI PREFERENTIAL EXPRESSION OF THE DBL PROTOONCOGENE IN SOME TUMORS OF NEUROECTODERMAL ORIGIN SO CANCER RESEARCH LA English DT Article ID NEURO-BLASTOMA; PROTO-ONCOGENE; EWINGS-SARCOMA; CELLS; DISTINCT; DIFFERENTIATION AB We have investigated the expression of the dbl protooncogene in a wide variety of human tumors of different embryological derivation. We found that proto-dbl mRNA could be detected preferentially in a few neoplastic histiotypes of neuroectodermal origin. The common, normal 5-kilobase size of the proto-dbl transcript detected indicated that the proto-dbl in these tumors was not rearranged. These data, in agreement with our previous reports, suggest that the dbl protooncogene is expressed in a highly tissue-specific manner and indicate that this gene may be involved in the growth and differentiation of some cells of neuroectodermal origin. C1 NAPLES UNIV,DIPARTIMENTO BIOL & PATOL CELLULARE & MOLEC L CALIFANO,CNR,I-80131 NAPLES,ITALY. NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892. RI Eva, Alessandra/J-8268-2016 OI Eva, Alessandra/0000-0003-2949-078X NR 22 TC 14 Z9 14 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD AUG 15 PY 1991 VL 51 IS 16 BP 4234 EP 4237 PG 4 WC Oncology SC Oncology GA GA543 UT WOS:A1991GA54300019 PM 1868443 ER PT J AU COOK, JA PASS, HI IYPE, SN FRIEDMAN, N DEGRAFF, W RUSSO, A MITCHELL, JB AF COOK, JA PASS, HI IYPE, SN FRIEDMAN, N DEGRAFF, W RUSSO, A MITCHELL, JB TI CELLULAR GLUTATHIONE AND THIOL MEASUREMENTS FROM SURGICALLY RESECTED HUMAN LUNG-TUMOR AND NORMAL LUNG-TISSUE SO CANCER RESEARCH LA English DT Article ID HUMAN OVARIAN-CANCER; CYTO-TOXICITY; DEPLETION; CELLS; LINES; TRANSFERASE; MONOCHLOROBIMANE; MONOBROMOBIMANE; XENOGRAFTS; HAMSTER AB Cellular glutathione (GSH) levels were measured from 27 human lung tumor biopsies, enzymatically disaggregated, and compared with cells isolated from normal lung of the same patients. GSH levels from normal lung were similar among patients with a mean value of 11.20 +/- 0.58 (SEM) nmol GSH/mg protein (24 patients) with a range from 6.1 to 17.5 nmol GSH/mg protein. GSH levels varied considerably within and across histological tumor types with the following values: adenocarcinomas, 8.83 +/- 0.96 nmol/mg protein (8 patients); large cell carcinomas, 8.25 +/- 2.51 nmol/mg protein (3 patients), and squamous cell carcinomas, 23.25 +/- 5.99 nmol/mg protein (8 patients). The cyclic GSH reductase assay gave only average GSH values and could not distinguish possible GSH variation among subpopulations of cells isolated. Cell volume measurements and microscopic evaluation of cells isolated from both tumors and normal lung revealed heterogeneity with respect to cell types present. To determine the extent of thiol variation among tumor cell subpopulations, tumor cell suspensions were stained with the thiol-specific stain, monochlorobimane (MCB). The accuracy of MCB staining was tested by flow cytometric analysis of 12 in vitro human tumor cell lines and 3 rodent cell lines. A linear relationship was found between the bimane cellular fluorescence and the cyclic GSH reductase assay for cell lines having less than 80 nmol GSH/mg protein (R2 = 0.82). Above 80 nmol GSH/mg protein the rate of change of the bimane fluorescence intensity with respect to increasing GSH concentrations was much reduced. However, by labeling cells with MCB it was possible to distinguish between cell lines with low versus high GSH content. MCB staining of tumor samples revealed multiple populations of cells with respect to thiol levels. In particular, 2 of 8 squamous cell carcinomas had a proportion of cells with elevated fluorescence intensities (from 10 to 35% of the population) suggesting the presence of cells with greatly elevated thiol levels. These findings underscore the complexity of quantitating intracellular GSH levels from tumor biopsies. The combined use of MCB with flow cytometry and conventional GSH assays may help to delineate subpopulations of cells within tumors with different thiol levels. C1 NCI,SURG BRANCH,BETHESDA,MD 20892. RP COOK, JA (reprint author), NCI,RADIAT ONCOL BRANCH,BLDG 10,ROOM B3-B69,BETHESDA,MD 20892, USA. NR 35 TC 101 Z9 101 U1 1 U2 6 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD AUG 15 PY 1991 VL 51 IS 16 BP 4287 EP 4294 PG 8 WC Oncology SC Oncology GA GA543 UT WOS:A1991GA54300027 PM 1868449 ER PT J AU TSUDA, M KIKUCHI, T YAMAKI, K SHINOHARA, T AF TSUDA, M KIKUCHI, T YAMAKI, K SHINOHARA, T TI THE MOUSE S-ANTIGEN GENE - COMPARISON WITH HUMAN AND DROSOPHILA SO EUROPEAN JOURNAL OF BIOCHEMISTRY LA English DT Article ID BETA-ADRENERGIC-RECEPTOR; ROD DISK MEMBRANES; 48-KDA PROTEIN; RHODOPSIN-KINASE; PINEAL-GLAND; CGMP PHOSPHODIESTERASE; INVITRO TRANSCRIPTION; SEQUENCE-ANALYSIS; BOVINE RETINA; ARRESTIN AB We have characterized a gene for mouse S-antigen and compared its sequence with that of corresponding human and two recently published Drosophila S-antigen genes. The mouse S-antigen gene was approximately 50 kbp in length and consisted of 16 exons and 15 introns. The length of most exons was less than 100 bp and the smallest one was only 10 bp. In contrast, the length of most introns was larger than 2 kbp and the gene consisted of 97% intron and 3% exon. Both splice sites for donor and accepter were in good agreement with the GT/AG rule. S-antigen genes in human and mouse were highly conserved. In contrast, genes for the Drosophila 49-kDa arrestin homolog and arrestin consist of three introns and four exons and two introns and three exons, respectively. The 5'-flanking region of the mouse S-antigen gene, approximately 1.0 kbp long, had no regulatory elements for transcription such as the TATA, CAAT and GC boxes, while a Drosophila arrestin gene has TATA and CAAT boxes. Interestingly, the 5'-flanking region of the mouse gene had promoter activity in an in vitro transcription assay using a nuclear extract of rat brain. A major transcription start site was found at 387 bp upstream from the translation start codon ATG in mouse. From our results, and those of others, we suggest that the S-antigen gene has evolved from a comman ancestor gene by either insertion or deletion of introns. Such an alteration of gene structure may have played a role in the evolution of the S-antigen. C1 NEI,RETINAL CELL & MOLEC BIOL LAB,MOLEC BIOL SECT,BLDG 10,RM 10N117,BETHESDA,MD 20892. OI Shinohara, Toshimichi/0000-0002-7197-9039 NR 44 TC 21 Z9 21 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0014-2956 J9 EUR J BIOCHEM JI Eur. J. Biochem. PD AUG 15 PY 1991 VL 200 IS 1 BP 95 EP 101 DI 10.1111/j.1432-1033.1991.tb21053.x PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA775 UT WOS:A1991GA77500013 PM 1879432 ER PT J AU LIN, HH KOUSVELARI, EE ANN, DK AF LIN, HH KOUSVELARI, EE ANN, DK TI SEQUENCE AND EXPRESSION OF THE MNP4 GENE ENCODING BASIC PROLINE-RICH PROTEIN IN MACAQUE SALIVARY-GLANDS SO GENE LA English DT Article DE RECOMBINANT DNA; MULTIGENE FAMILY; CONTIGUOUS REPEATS; TISSUE-SPECIFIC GENE EXPRESSION ID MULTIGENE FAMILY; RAT; DNA; ORGANIZATION; CELLS; RNA; CDNA AB We report here the macaque MnP4 cDNA and genomic sequences which encode a basic proline-rich protein (PRP), which is synthesized in macaque parotid gland and submandibular gland. The locations of intron positions and the prototype of the tandem 20-amino-acid repeat motif with the sequence, PPPPGKPQGPPQQGGNKPQG, in MnP4, were compared to those in related genes encoding PRP and glutamic/glutamine-rich proteins (GRP) in humans and rodents. Exceedingly high homology of the first exon and 40-bp region immediately upstream of exon I is observed with other PRP genes of all species studied. In order to identify the regulatory elements involved in control of MnP4 gene expression, a rat submandibular gland-derived cell line (RSMT-A5) was transfected with MnP4-cat constructs that contained the promoter and 5'-flanking regions of the macaque MnP4 gene fused to the bacterial cat gene. Deletion analysis revealed that putative positive and negative regulatory elements reside between nucleotides (nt) -107 and +5, and nt -586 and -108, respectively. As part of this study, the promoter of the macaque MnP4 gene appears to be salivary gland specific. This salivary gland-specific gene expression attests to the complexity of transcriptional regulation in eukaryotes. C1 UNIV MINNESOTA,SCH MED,DEPT PHARMACOL,3-249 MILLARD HALL,MINNEAPOLIS,MN 55455. NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892. FU NIDCR NIH HHS [5R29-DE09175, K04-DE00292] NR 26 TC 9 Z9 9 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 J9 GENE JI Gene PD AUG 15 PY 1991 VL 104 IS 2 BP 219 EP 226 DI 10.1016/0378-1119(91)90253-8 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA GJ965 UT WOS:A1991GJ96500012 PM 1916292 ER PT J AU BENOVIC, JL ONORATO, JJ ARRIZA, JL STONE, WC LOHSE, M JENKINS, NA GILBERT, DJ COPELAND, NG CARON, MG LEFKOWITZ, RJ AF BENOVIC, JL ONORATO, JJ ARRIZA, JL STONE, WC LOHSE, M JENKINS, NA GILBERT, DJ COPELAND, NG CARON, MG LEFKOWITZ, RJ TI CLONING, EXPRESSION, AND CHROMOSOMAL LOCALIZATION OF BETA-ADRENERGIC-RECEPTOR KINASE-2 - A NEW MEMBER OF THE RECEPTOR KINASE FAMILY SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID LIGHT-DEPENDENT PHOSPHORYLATION; PROTEIN-KINASE; ADENYLATE-CYCLASE; RHODOPSIN KINASE; CHICK HEART; HOMOLOGOUS DESENSITIZATION; BETA-2-ADRENERGIC RECEPTOR; DICTYOSTELIUM-DISCOIDEUM; CHEMOTACTIC RECEPTOR; S49-LYMPHOMA CELLS AB The beta-adrenergic receptor kinase (beta-ARK) specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Structural features of this enzyme have been elucidated recently by the isolation of a cDNA that encodes bovine-beta-ARK. Utilizing a catalytic domain fragment of the beta-ARK cDNA to screen a bovine brain cDNA library we have isolated a clone encoding a beta-ARK-related enzyme which we have termed beta-ARK2. Overall, this enzyme has 85% amino acid identity with beta-ARK, with the protein kinase catalytic domain having 95% identity. The ability of beta-ARK2 to phosphorylate various substrates was studied after expression in COS 7 cells. Although beta-ARK2 is essentially equiactive with beta-ARK in phosphorylating an acid-rich synthetic model peptide it was only approximately 50% as active when the substrate was the agonist-occupied beta-2-adrenergic receptor and only approximately 20% as active toward light-bleached rhodopsin. As with beta-ARK, phosphorylation of the receptor substrates by beta-ARK2 was completely stimulus dependent. RNA blot analysis with selected bovine tissues reveals an mRNA of 8 kilobases with a distribution similar to that of beta-ARK. More detailed RNA analysis using a ribonuclease protection assay in various rat tissues suggests that the beta-ARK2 message is present at much lower levels (typically 10-20%) than the beta-ARK message. In the rat the beta-ARK2 mRNA is localized predominantly in neuronal tissues although low levels are also observed in various peripheral tissues. The beta-ARK2 gene has been localized to a region of mouse chromosome 5 whereas the beta-ARK gene is localized on mouse chromosome 19. These data suggest the existence of a ''family'' of receptor kinases which may serve broadly to regulate receptor function. C1 DUKE UNIV,MED CTR,HOWARD HUGHES MED INST,DURHAM,NC 27710. TEMPLE UNIV,HLTH SCI CTR,SCH MED,INST CANC RES & MOLEC BIOL,PHILADELPHIA,PA 19140. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,FREDERICK,MD 21702. RI Lohse, Martin/A-7160-2012 OI Lohse, Martin/0000-0002-0599-3510 FU NCI NIH HHS [N01-CO-74101]; NHLBI NIH HHS [HL16037]; NIGMS NIH HHS [GM44944] NR 58 TC 201 Z9 204 U1 1 U2 4 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 15 PY 1991 VL 266 IS 23 BP 14939 EP 14946 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB097 UT WOS:A1991GB09700024 PM 1869533 ER PT J AU CARROLL, MP SPIVAK, JL MCMAHON, M WEICH, N RAPP, UR MAY, WS AF CARROLL, MP SPIVAK, JL MCMAHON, M WEICH, N RAPP, UR MAY, WS TI ERYTHROPOIETIN INDUCES RAF-1 ACTIVATION AND RAF-1 IS REQUIRED FOR ERYTHROPOIETIN-MEDIATED PROLIFERATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PROTEIN KINASE-C; GENE-EXPRESSION; CELL-GROWTH; PHOSPHORYLATION; RECEPTOR; RNA; INTERLEUKIN-3; PROTOONCOGENE; COMPLEMENTARY; MEMBRANE AB Erythropoietin mediates the rapid phosphorylation of Raf-1 in the murine cell lines HCD-57 and FDC-P1/ER, which proliferate in response to this cytokine. Phosphorylation occurs at both serine and tyrosine residues and as such is similar to the Raf-1 phosphorylation seen after interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor, and interleukin-2 stimulation in other murine cell lines. Such data suggest that these growth factors may share a common mechanism(s) of Raf-1 phosphorylation. Furthermore, in association with Raf-1 phosphorylation, erythropoietin induces a 2-3-fold increase in Raf-1 kinase activity as measured in immune complex kinase assays in vitro. Finally, a c-raf antisense oligodeoxyribonucleotide, which specifically decreases intracellular Raf-I levels, also substantially inhibits both erythropoietin and IL-3-directed DNA synthesis. Together, these results provide evidence that activated Raf-1 is a necessary component of erythropoietin and IL-3 growth signaling pathways. C1 JOHNS HOPKINS UNIV,SCH MED,JOHNS HOPKINS ONCOL CTR,424 N BOND ST,BALTIMORE,MD 21231. JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV HEMATOL,BALTIMORE,MD 21231. JOHNSON & JOHNSON,RW JOHNSON PHARMACEUT RES INST,RARITAN,NJ 08869. NCI,FREDERICK CANC RES & DEV CTR,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702. FU NCI NIH HHS [CA 44649, CA 47993]; NIDDK NIH HHS [DK 16702] NR 47 TC 170 Z9 170 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 15 PY 1991 VL 266 IS 23 BP 14964 EP 14969 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB097 UT WOS:A1991GB09700028 PM 1869534 ER PT J AU KOMORIYA, A GREEN, LJ MERVIC, M YAMADA, SS YAMADA, KM HUMPHRIES, MJ AF KOMORIYA, A GREEN, LJ MERVIC, M YAMADA, SS YAMADA, KM HUMPHRIES, MJ TI THE MINIMAL ESSENTIAL SEQUENCE FOR A MAJOR CELL TYPE-SPECIFIC ADHESION SITE (CS1) WITHIN THE ALTERNATIVELY SPLICED TYPE-III CONNECTING SEGMENT DOMAIN OF FIBRONECTIN IS LEUCINE-ASPARTIC ACID-VALINE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HUMAN-PLASMA FIBRONECTIN; SURFACE RECEPTORS; IDENTIFICATION; ATTACHMENT; FRAGMENTS; INTEGRINS; REGION AB Fibronectin contains at least two major domains that support cell adhesion. One is the central cell-binding domain that is recognized by a variety of cell types via the integrin-alpha-5-beta-1. The second, originally identified by its ability to support melanoma cell adhesion, is located in the alternatively spliced type III connecting segment (IIICS). A dominant cell type-specific adhesion site within the IIICS has been localized to a peptide designated as CS1 comprising its amino-terminal 25 residues. The receptor for CS1 is the integrin-alpha-4-beta-1. We have synthesized a variety of peptides with overlapping sequences in order to identify the minimum active amino acid sequence of this major cell adhesion site. A peptide comprising the carboxyl-terminal 8 amino acids of CS1, EILDVPST, was found to support melanoma cell spreading, while all peptides without this sequence had little or no activity. Two smaller overlapping pentapeptides, EILDV and LDVPS, were also active, whereas EILEV, containing a conservative substitution of Glu for Asp, was inactive. These data suggested that the minimum sequence for cell adhesion activity is Leu-Asp-Val, the tripeptide sequence common to both active peptides. This prediction was confirmed by the observed ability of the Leu-Asp-Val peptide itself to block spreading on fibronectin, whereas Leu-Glu-Val was inactive. Interspecies amino acid sequence comparison also supports the importance of the LDV sequence, since it is completely conserved in the IIICS regions of human, rat, bovine, and avian fibronectins. C1 UNIV MANCHESTER,DEPT BIOCHEM & MOLEC BIOL,MANCHESTER M13 9PT,LANCS,ENGLAND. RORER GRP INC,KING OF PRUSSIA,PA 19406. NCI,MOLEC BIOL LAB,MEMBRANE BIOCHEM SECT,BETHESDA,MD 20892. RP KOMORIYA, A (reprint author), US FDA,CTR BIOL EVALUAT & RES,DIV CYTOKINE BIOL,CELL BIOL LAB,8000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. OI Yamada, Kenneth/0000-0003-1512-6805 FU Wellcome Trust NR 20 TC 298 Z9 302 U1 1 U2 11 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 15 PY 1991 VL 266 IS 23 BP 15075 EP 15079 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB097 UT WOS:A1991GB09700044 PM 1869542 ER PT J AU HIDE, M BEAVEN, MA AF HIDE, M BEAVEN, MA TI CALCIUM INFLUX IN A RAT MAST-CELL (RBL-2H3) LINE - USE OF MULTIVALENT METAL-IONS TO DEFINE ITS CHARACTERISTICS AND ROLE IN EXOCYTOSIS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID BASOPHILIC LEUKEMIA-CELLS; PROTEIN-KINASE-C; ANAPHYLACTIC HISTAMINE SECRETION; CROMOLYN BINDING-PROTEIN; INOSITOL 1,4,5-TRISPHOSPHATE; SYNERGISTIC SIGNALS; 2H3 CELLS; IMMUNOLOGICAL STIMULUS; MEDIATOR RELEASE; IMMUNOGLOBULIN-E AB An increase in concentration of cytosolic Ca2+ ([Ca2+]i) is associated with an accelerated influx of Ca-45(2+) when cultured RBL-2H3 cells are stimulated with either antigen or analogs of adenosine although these agents act via different receptors and coupling proteins (Ali, H., Cunha-Melo, J. R., Saul, W. F., and Beaven, M. A. (1990) J. Biol. Chem. 265, 745-753). The same mechanism probably operates for basal Ca2+ influx in unstimulated cells and for the accelerated influx in stimulated cells. This influx had the following characteristics. 1) It was decreased when cells were depolarized with high external K+; 2) it was blocked by other cations (La3+ > Zn2+ > Cd2+ > Mn2 = Co2+ > Ba2+ > Ni2+ > Sr2+) either by competing with Ca2+ at external sites (e.g. La3+ or Zn2+) or by co-passage into the cell (e.g. Mn2+ or Sr2+); and 3) the inhibition of influx by K+ and the metal ions had exactly the same characteristics whether cells were stimulated or unstimulated even though influx rates were different. The dependence of various cellular responses on influx of Ca2+ was demonstrated as follows. The stimulated influx of Ca2+, rise in [Ca2+]i, and secretion, could be blocked in a concentration-dependent manner by increasing the concentration of La3+, but concentrations of La3+ (> 20-mu-M) that suppressed influx to below basal rates of influx markedly suppressed the hydrolysis of inositol phospholipids (levels of inositol 1,4,5-trisphosphate were unaffected). Some metal ions, e.g. Mn2+ and Sr2+, however, supported the stimulated hydrolysis of inositol phospholipid and some secretion in the absence of Ca2+. Thus a basal rate of influx of Ca2+ was required for the full activation of inositol phospholipid hydrolysis, but in addition an accelerated influx was necessary for exocytosis. C1 NHLBI,CHEM PHARMACOL LAB,BLDG 10,8N114,BETHESDA,MD 20892. NR 54 TC 69 Z9 69 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 15 PY 1991 VL 266 IS 23 BP 15221 EP 15229 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB097 UT WOS:A1991GB09700062 PM 1869551 ER PT J AU VANCOMPERNOLLE, K VANDEKERCKHOVE, J BUBB, MR KORN, ED AF VANCOMPERNOLLE, K VANDEKERCKHOVE, J BUBB, MR KORN, ED TI THE INTERFACES OF ACTIN AND ACANTHAMOEBA ACTOBINDIN - IDENTIFICATION OF A NEW ACTIN-BINDING MOTIF SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID AMINO-ACID-SEQUENCE; MYOSIN SUBFRAGMENT-1; CROSS-LINKING; ALPHA-ACTININ; HEAVY-CHAIN; PROTEINS; TROPOMYOSIN; COMPLEX; PLASTIN; DOMAINS AB Actobindin is an 88-amino acid polypeptide, containing two almost identical repeated domains of 33 and 34 residues. Depending on the molar ratios in which they are mixed, actobindin binds either one or two actin molecules. We cross-linked actobindin and actin in the 1:1 complex, using the zero-length cross-linker 1-ethyl-3(3-dimethylaminopropyl)carbodiimide. The cross-linked peptides were purified after consecutive CNBr cleavage and trypsin and Staphylococcus protease V8 digestions, and the cross-linked side chains were identified by amino acid sequencing. Isopeptide linkages were formed between residues Glu-100 of actin and Lys-16 of actobindin. In addition, we found a connection between one or more of the acidic residues 1, 2, or 3 of actin and Lys- 16 and Lys- 52 of actobindin. The cross-linked regions in actobindin contain Leu-Lys-His-Ala-Glu-Thr motifs, similar to sequences observed in several other actin-binding proteins. C1 NHLBI, CELL BIOL LAB, BETHESDA, MD 20892 USA. RP STATE UNIV GHENT, PHYSIOL CHEM LAB, KL LEDEGANCKSTR 35, B-9000 GHENT, BELGIUM. RI Korn, Edward/F-9929-2012 NR 30 TC 71 Z9 72 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 15 PY 1991 VL 266 IS 23 BP 15427 EP 15431 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB097 UT WOS:A1991GB09700089 PM 1869561 ER PT J AU SINGH, Y KLIMPEL, KR QUINN, CP CHAUDHARY, VK LEPPLA, SH AF SINGH, Y KLIMPEL, KR QUINN, CP CHAUDHARY, VK LEPPLA, SH TI THE CARBOXYL-TERMINAL END OF PROTECTIVE ANTIGEN IS REQUIRED FOR RECEPTOR-BINDING AND ANTHRAX TOXIN ACTIVITY SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID DEPENDENT ADENYLATE-CYCLASE; BACILLUS-ANTHRACIS; MOLECULAR-CLONING; LETHAL TOXIN; EDEMA FACTOR; ESCHERICHIA-COLI; FACTOR GENE; EXPRESSION; SEQUENCE; CELLS AB Anthrax toxin consists of three separate proteins produced by Bacillus anthracis: protective antigen (PA), lethal factor (LF), and edema factor (EF). Previous work showed that the process by which these proteins damage eukaryotic cells begins with binding of PA (83 kDa) to cell surface receptors. PA is then cleaved by a cell surface protease so as to expose a high-affinity binding site for LF or EF on the COOH-terminal, receptor-bound, 63-kilodalton fragment. In this report we more closely define a region of PA involved in receptor binding. The gene encoding PA was mutagenized so as to delete 3, 5, 7, 12, or 14 amino acids from the carboxyl terminus of the protein, and the truncated PA variants were purified from Bacillus subtilis or Escherichia coli. Deletion of 3, 5, or 7 amino acids reduced the binding of PA to cells and the subsequent toxicity of the PA.LF complex to J774A.1 cells and also the ability to cause EF binding to cells. Deletion of 12 or 14 amino acids completely eliminated all these activities. These results show that the carboxy terminus comprises or is part of the receptor-binding domain of PA. C1 NIDR,MICROBIAL ECOL LAB,BLDG 30,RM 309,BETHESDA,MD 20892. NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 32 TC 68 Z9 72 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 15 PY 1991 VL 266 IS 23 BP 15493 EP 15497 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB097 UT WOS:A1991GB09700099 PM 1651334 ER PT J AU BREITMAN, ML TSUDA, M USUKURA, J KIKUCHI, T ZUCCONI, A KHOO, W SHINOHARA, T AF BREITMAN, ML TSUDA, M USUKURA, J KIKUCHI, T ZUCCONI, A KHOO, W SHINOHARA, T TI EXPRESSION OF S-ANTIGEN IN RETINA, PINEAL-GLAND, LENS, AND BRAIN IS DIRECTED BY 5'-FLANKING SEQUENCES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID BETA-ADRENERGIC-RECEPTOR; 48-KDA PROTEIN; ALPHA-TRANSDUCIN; RHODOPSIN-KINASE; BOVINE RETINA; ARRESTIN; BINDING; DROSOPHILA; GENE; CDNA AB S-antigen (S-Ag) is an abundant protein of the retina and pineal gland that elicits experimental autoimmune uveitis and pinealocytis in several animal species. To study the elements regulating the expression of S-Ag, we generated transgenic mice expressing the chloramphenicol acetyl transferase (CAT) gene under the control of a 1.3-kilobase pair 5'-flanking segment of the mouse S-Ag gene. While all of the transgenic mice expressed CAT activity in the retina, in some animals CAT activity was also detected in the pineal gland, lens, and brain. Immunoblotting, polymerase chain reaction-mediated detection of RNA, and immunocytostaining of transgenic tissues with antibodies to CAT and S-Ag established that the profile of expression of the transgene corresponded to that of S-Ag; both proteins were detectable in retinal photoreceptor cells, pinealocytes, lens fiber and epithelial cells, the cerebellum, and the cerebral cortex. These results indicate that S-Ag is expressed in a wider spectrum of the cell types than previously recognized and that a 1.3-kilobase pair S-Ag promoter segment contains sufficient information to direct appropriate tissue-specific gene expression in transgenic mice. C1 MT SINAI HOSP,SAMUEL LUNENFELD RES INST,DIV MOLEC & DEV BIOL,600 UNIV AVE,TORONTO M5G 1X5,ONTARIO,CANADA. UNIV TORONTO,DEPT MOLEC & MED GENET,TORONTO M5S 1A1,ONTARIO,CANADA. NAGOYA UNIV,SCH MED,DEPT ANAT,NAGOYA,AICHI 466,JAPAN. NEI,RETINAL CELL & MOLEC BIOL LAB,BETHESDA,MD 20892. NR 41 TC 40 Z9 40 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 15 PY 1991 VL 266 IS 23 BP 15505 EP 15510 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB097 UT WOS:A1991GB09700101 PM 1714458 ER PT J AU BROYLES, SS LI, J MOSS, B AF BROYLES, SS LI, J MOSS, B TI PROMOTER DNA CONTACTS MADE BY THE VACCINIA VIRUS EARLY TRANSCRIPTION FACTOR SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID DEPENDENT RNA-POLYMERASE; ESCHERICHIA-COLI; THYMIDINE KINASE; EARLY GENES; PURIFICATION; VIRIONS; SEQUENCES; BINDING; REGION AB Vaccinia virus RNA polymerase requires the heterodimeric protein, vaccinia early transcription factor (VETF), for transcription of early gene templates in vitro. We have analyzed the vaccinia growth factor promoter sequences interacting with VETF at the nucleotide level and provide evidence that the factor contacts the DNA at two separate sites. DNase I protection analysis showed that VETF was bound to nucleotides -12 to -29 relative to the transcription initiation site, and also to nucleotides +8 to +10 downstream of the initiation site. The importance of both binding sites for stable complex formation was supported by methylation interference analysis. Using synthetic oligonucleotides encoding different parts of the vaccinia growth factor promoter, it was shown that nucleotides downstream of the transcription initiation site are required for stable complex formation. Competition binding experiments demonstrated that only the upstream binding site contributes significantly to binding specificity. Binding to two separated DNA sequences results in a bend in the promoter DNA as demonstrated by electrophoretic mobility shift analysis of permuted DNA fragments. These findings suggest that VETF activates transcription by sequence specific binding and structural alteration of the promoter DNA helix. C1 NIAID, VIRAL DIS LAB, BETHESDA, MD 20892 USA. RP BROYLES, SS (reprint author), PURDUE UNIV, DEPT BIOCHEM, W LAFAYETTE, IN 47907 USA. FU NIAID NIH HHS [1R01-AI28432-02] NR 25 TC 32 Z9 32 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 15 PY 1991 VL 266 IS 23 BP 15539 EP 15544 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB097 UT WOS:A1991GB09700106 PM 1869571 ER PT J AU SZABO, A AF SZABO, A TI THEORETICAL APPROACHES TO REVERSIBLE DIFFUSION-INFLUENCED REACTIONS - MONOMER EXCIMER KINETICS SO JOURNAL OF CHEMICAL PHYSICS LA English DT Article AB Three formalisms that describe the influence of diffusion on the kinetics of the reversible reaction, A + B-reversible-AB, are discussed and compared. The simplest involves a modification of the irreversible rate equations of Smoluchowski theory; the second is based on a generalization of physically appealing convolution relations that hold rigorously for reversible reactions between isolated pairs, and the third can be obtained by using a superposition approximation to truncate the hierarchy of equations satisfied by the reactive reduced distribution functions. The various formalisms are developed to the point that their implementation requires knowledge only of the time-dependent irreversible association rate coefficient and the microscopic dissociation rate constant. All these approaches give the correct equilibrium concentrations at infinite time, have the same short-time behavior, reduce correctly when the dissociation rate is zero, and become equilvalent in the reaction-controlled limit. However, none of them provides an exact treatment of the underlying many-particle diffusive model of the reaction. Some illustrative calculations are presented and the relative merits of these approaches are discussed. All three approaches predict that the relaxation of a small initial deviation of the concentrations from their equilibrium values is nonexponential, except, of course, in the reaction-controlled limit. With a view towards treating monomer-excimer kinetics, the formalisms are generalized to incorporate unimolecular decay pathways. RP SZABO, A (reprint author), NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892, USA. RI Szabo, Attila/H-3867-2012 NR 22 TC 127 Z9 127 U1 4 U2 10 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0021-9606 J9 J CHEM PHYS JI J. Chem. Phys. PD AUG 15 PY 1991 VL 95 IS 4 BP 2481 EP 2490 DI 10.1063/1.460952 PG 10 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA GA108 UT WOS:A1991GA10800027 ER PT J AU HAKIMI, J CHIZZONITE, R LUKE, DR FAMILLETTI, PC BAILON, P KONDAS, JA PILSON, RS LIN, P WEBER, DV SPENCE, C MONDINI, LJ TSIEN, WH LEVIN, JL GALLATI, VH KORN, L WALDMANN, TA QUEEN, C BENJAMIN, WR AF HAKIMI, J CHIZZONITE, R LUKE, DR FAMILLETTI, PC BAILON, P KONDAS, JA PILSON, RS LIN, P WEBER, DV SPENCE, C MONDINI, LJ TSIEN, WH LEVIN, JL GALLATI, VH KORN, L WALDMANN, TA QUEEN, C BENJAMIN, WR TI REDUCED IMMUNOGENICITY AND IMPROVED PHARMACOKINETICS OF HUMANIZED ANTI-TAC IN CYNOMOLGUS MONKEYS SO JOURNAL OF IMMUNOLOGY LA English DT Article ID RECEPTOR MONOCLONAL-ANTIBODY; INTERLEUKIN-2 RECEPTOR; MULTIPLE INJECTIONS; ALLOGRAFT SURVIVAL; IMMUNE-RESPONSE; RHESUS-MONKEYS; HUMAN CONSTANT; T-CELLS; MOUSE; THERAPY AB The anti-Tac mAb has been shown to bind to the p55 chain of the IL-2R, block IL-2 binding and inhibit T cell proliferation. A humanized form of anti-Tac (HAT) has been constructed that retains the binding properties of murine anti-Tac (MAT). These two mAb were evaluated in cynomolgus monkeys to compare relative immunogenicity and pharmacokinetic properties. Monkeys treated with HAT daily for 14 days exhibited anti-HAT antibody titers which were 5- to 10-fold lower than their MAT-treated counterparts and these antibodies developed later than in the MAT-treated monkeys. Two of four monkeys receiving a single injection of MAT developed anti-MAT antibodies, whereas none of four monkeys developed antibodies after a single treatment with HAT. In monkeys injected with either HAT or MAT daily for 14 days, the anti-antibody titers induced were inversely related to the amount of anti-Tac administered. Antibodies that developed against MAT were both anti-isotypic and anti-idiotypic, whereas those developed against HAT appeared to be predominantly anti-idiotypic. The pharmacokinetic properties, that is the half-life and area under the curve values, of HAT were also significantly different from those of MAT. The area under the curve values for HAT in naive monkeys were approximately twofold more than those for MAT, and the mean serum half-life of HAT was 214 h, approximately four- to fivefold more than MAT. These pharmacokinetic values were reduced in monkeys previously sensitized with HAT or MAT suggesting that the presence of anti-antibodies altered these parameters. C1 HOFFMANN LA ROCHE INC,ROCHE RES CTR,DEPT MOLEC GENET,NUTLEY,NJ 07110. HOFFMANN LA ROCHE INC,ROCHE RES CTR,DEPT DRUG METAB,NUTLEY,NJ 07110. HOFFMANN LA ROCHE INC,ROCHE RES CTR,DEPT BIOPROC DEV,NUTLEY,NJ 07110. HOFFMANN LA ROCHE INC,ROCHE RES CTR,DEPT PROT BIOCHEM,NUTLEY,NJ 07110. TSI MASON RES INST,WORCESTER,MA 01608. F HOFFMANN LA ROCHE & CO LTD,CENT RES,CH-4002 BASEL,SWITZERLAND. PROT DESIGN LABS INC,MT VIEW,CA 94043. NCI,METAB BRANCH,BETHESDA,MD 20892. RP HAKIMI, J (reprint author), HOFFMANN LA ROCHE INC,ROCHE RES CTR,DEPT IMMUNOPHARMACOL,NUTLEY,NJ 07110, USA. NR 41 TC 103 Z9 105 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD AUG 15 PY 1991 VL 147 IS 4 BP 1352 EP 1359 PG 8 WC Immunology SC Immunology GA GB209 UT WOS:A1991GB20900035 PM 1869828 ER PT J AU RESTIFO, NP ESQUIVEL, F ASHER, AL STOTTER, H BARTH, RJ BENNINK, JR MULE, JJ YEWDELL, JW ROSENBERG, SA AF RESTIFO, NP ESQUIVEL, F ASHER, AL STOTTER, H BARTH, RJ BENNINK, JR MULE, JJ YEWDELL, JW ROSENBERG, SA TI DEFECTIVE PRESENTATION OF ENDOGENOUS ANTIGENS BY A MURINE SARCOMA - IMPLICATIONS FOR THE FAILURE OF AN ANTITUMOR IMMUNE-RESPONSE SO JOURNAL OF IMMUNOLOGY LA English DT Article ID TOXIC LYMPHOCYTES-T; MAJOR HISTOCOMPATIBILITY COMPLEX; CLASS-I MOLECULES; H-2-DEFICIENT LYMPHOMA VARIANTS; INFILTRATING LYMPHOCYTES; RECOMBINANT INTERLEUKIN-2; INTRACELLULAR-TRANSPORT; ADOPTIVE IMMUNOTHERAPY; MONOCLONAL-ANTIBODIES; HYBRIDOMA ANTIBODIES AB MHC class I-restricted CTL play a central role in the immune response against methylcholanthrene (MCA)-induced sarcomas in mice. We, therefore, hypothesized that MCA-induced tumors may evade immune recognition by failing to present Ag to CD8+ CTL. Of a number of previously described MCA-induced sarcomas, one, MCA 101, fails to induce CTL, is nonimmunogenic, and grows rapidly and lethally in nonimmunosuppressed recipients. To better understand the nonimmunogenicity of MCA 101 we examined its ability to present foreign Ag to CTL. Unlike immunogenic sarcomas, MCA 101 failed to present endogenously synthesized influenza virus Ag to influenza virus-specific CTL. The deficiency in presentation of endogenous Ag by MCA 101 was attributed to a markedly reduced rate of synthesis of class I molecules because up-regulation of class I synthesis by IFN-gamma greatly increased the presentation of influenza A virus Ag. Despite low levels of cell surface class I expression, MCA 101 presented exogenous peptide Ag to anti-influenza CTL with efficiency similar to immunogenic MCA sarcoma cell lines. These findings could not be attributed to deficiencies in class I assembly or transport, as has been suggested by others who have studied mutant cells with defective Ag presentation. Furthermore, our studies suggest that some tumor cells can escape recognition by CTL and subsequent immune eradication by suppressing presentation of endogenous Ag. C1 NIAID,DIV INTRAMURAL RES,VIRAL DIS LAB,ROCKVILLE,MD 20852. RP RESTIFO, NP (reprint author), NCI,DIV CANC TREATMENT,SURG BRANCH,BLDG 10,ROOM 2B-42,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Restifo, Nicholas/A-5713-2008; yewdell, jyewdell@nih.gov/A-1702-2012; OI Restifo, Nicholas P./0000-0003-4229-4580 FU Intramural NIH HHS [Z01 BC010763-01, Z99 CA999999] NR 46 TC 113 Z9 115 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD AUG 15 PY 1991 VL 147 IS 4 BP 1453 EP 1459 PG 7 WC Immunology SC Immunology GA GB209 UT WOS:A1991GB20900050 PM 1907999 ER PT J AU BASILE, AS HUGHES, RD HARRISON, PM MURATA, Y PANNELL, L JONES, EA WILLIAMS, R SKOLNICK, P AF BASILE, AS HUGHES, RD HARRISON, PM MURATA, Y PANNELL, L JONES, EA WILLIAMS, R SKOLNICK, P TI ELEVATED BRAIN CONCENTRATIONS OF 1,4-BENZODIAZEPINES IN FULMINANT HEPATIC-FAILURE SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID BENZODIAZEPINE RECEPTOR LIGANDS; GAMMA-AMINOBUTYRIC-ACID; ANIMAL-MODEL; RABBIT MODEL; ANTAGONIST FLUMAZENIL; ENCEPHALOPATHY; COMPLEX; REVERSAL; DIAZEPAM; POTATO AB Background. Increased gamma-aminobutyric acid (GABA) neurotransmission has been implicated in the pathogenesis of hepatic encephalopathy. The mechanism by which GABA-ergic activity is increased in hepatic failure is unclear, but recent studies in animals with encephalopathy due to fulminant hepatic failure suggest that GABA-ergic neurotransmission may be increased by the presence of elevated concentrations of benzodiazepine agonists such as diazepam and N-desmethyldiazepam. Methods and Results. Samples of frontal cortex were obtained at autopsy from 11 patients with hepatic encephalopathy who died of acetaminophen-induced fulminant hepatic failure and 8 patients who died of cardiovascular disease or trauma. None of the 19 patients had received benzodiazepines while hospitalized. Chromatographic analyses of extracts of these samples revealed 4 to 19 peaks representing substances that inhibited the binding of a radiolabeled imidazobenzodiazepine ([H-3]flumazenil) to its receptors. Several of these peaks had retention times corresponding to those of known 1,4-benzodiazepines. Ultraviolet- and mass-spectroscopic analysis confirmed that two of these peaks represented diazepam and N-desmethyldiazepam. The patients who died of fulminant hepatic failure could be divided into two groups: six who had had significantly elevated brain concentrations (2-fold to 10-fold higher than normal) of substances inhibiting the binding of [H-3]flumazenil and five who had normal concentrations. Conclusions. Brain concentrations of substances inhibiting the binding of [H-3]flumazenil to its receptors are increased in some patients with hepatic encephalopathy due to fulminant hepatic failure. The origin of these substances is unknown, but these findings provide a rational basis for trials of benzodiazepine-receptor antagonists in the management of this disorder. C1 NIDDKD,DIGEST DIS BRANCH,BIOORGAN CHEM LAB,BETHESDA,MD 20892. NIDDKD,DIGEST DIS BRANCH,LIVER DIS SECT,BETHESDA,MD 20892. UNIV LONDON KINGS COLL,SCH MED & DENT,LIVER UNIT,LONDON WC2R 2LS,ENGLAND. RP BASILE, AS (reprint author), NIDDKD,DIGEST DIS BRANCH,NEUROSCI LAB,BLDG 8,RM 111,BETHESDA,MD 20892, USA. RI Harrison, Phillip/D-5374-2009 NR 35 TC 131 Z9 132 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD AUG 15 PY 1991 VL 325 IS 7 BP 473 EP 478 DI 10.1056/NEJM199108153250705 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA GA763 UT WOS:A1991GA76300005 PM 1649403 ER PT J AU PENG, CK HAVLIN, S SCHWARTZ, M STANLEY, HE AF PENG, CK HAVLIN, S SCHWARTZ, M STANLEY, HE TI DIRECTED-POLYMER AND BALLISTIC-DEPOSITION GROWTH WITH CORRELATED NOISE SO PHYSICAL REVIEW A LA English DT Note ID RENORMALIZATION-GROUP ANALYSIS; BURGERS-EQUATION; RANDOM-MEDIA; INTERFACES; SURFACES; MODEL AB We present numerical studies of the effect of long-range correlated noise on (i) the nonlinear Kardar, Parisi, and Zhang (KPZ) stochastic differential equation and the related problem of directed-polymer (DP) growth, and (ii) the ballistic-deposition (BD) model. The results for the KPZ and DP models are consistent with each other, and agree better with one recent theoretical prediction of Hentschel and Family [Phys. Rev. Lett. 66, 1982 (1991)] than with other theoretical predictions. Contrary to the general belief that BD is described by the KPZ equation, we find the surprising result that BD with correlated noise belongs to a different universality class than the KPZ equation. C1 BOSTON UNIV,DEPT PHYS,BOSTON,MA 02215. NIH,DIV COMP RES & TECHNOL,PHYS SCI LAB,BETHESDA,MD 20892. TEL AVIV UNIV,DEPT PHYS & ASTRON,IL-69978 TEL AVIV,ISRAEL. RP PENG, CK (reprint author), BOSTON UNIV,CTR POLYMER STUDIES,BOSTON,MA 02215, USA. RI Peng, Chung-Kang/E-1489-2011 OI Peng, Chung-Kang/0000-0003-3666-9833 NR 25 TC 86 Z9 86 U1 0 U2 6 PU AMERICAN PHYSICAL SOC PI COLLEGE PK PA ONE PHYSICS ELLIPSE, COLLEGE PK, MD 20740-3844 USA SN 1050-2947 J9 PHYS REV A JI Phys. Rev. A PD AUG 15 PY 1991 VL 44 IS 4 BP R2239 EP R2242 PG 4 WC Optics; Physics, Atomic, Molecular & Chemical SC Optics; Physics GA GC350 UT WOS:A1991GC35000004 ER PT J AU LESCH, KP AULAKH, CS TOLLIVER, TJ HILL, JL WOLOZIN, BL MURPHY, DL AF LESCH, KP AULAKH, CS TOLLIVER, TJ HILL, JL WOLOZIN, BL MURPHY, DL TI DIFFERENTIAL-EFFECTS OF LONG-TERM LITHIUM AND CARBAMAZEPINE ADMINISTRATION ON GS-ALPHA-PROTEIN AND GI-ALPHA-PROTEIN IN RAT-BRAIN SO EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION LA English DT Note DE G-PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA); LITHIUM; CARBAMAZEPINE ID GTP-BINDING AB The G protein subunits, G(s-alpha), G(i-alpha), and G(o-alpha), have been quantitated in various rat brain regions using enzyme-linked immunosorbent assay (ELISA) techniques. Chronic (3-week) treatment with carbamazepine decreased G(s-alpha) in several brain regions reaching significance in the neostriatum, while chronic lithium treatment had no unequivocal effect. Lithium significantly increased G(i-alpha) in the hypothalamus and hippocampus, whereas carbamazepine decreased G(i-alpha) in the frontal cortex. Both treatments had no consistent effects on G(o-alpha). We conclude that long-term treatment with lithium and carbamazepine exerts differential effects on G protein alpha-subunits, and that this modification of signal transduction represents a potential mechanism of antibipolar drug-induced neural plasticity. RP LESCH, KP (reprint author), NIMH,CTR CLIN,CLIN SCI LAB,10-3D41,BETHESDA,MD 20892, USA. RI Lesch, Klaus-Peter/J-4906-2013 OI Lesch, Klaus-Peter/0000-0001-8348-153X NR 12 TC 51 Z9 51 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0922-4106 J9 EUR J PHARM-MOLEC PH JI Eur. J. Pharmacol.-Molec. Pharmacol. Sect. PD AUG 14 PY 1991 VL 207 IS 4 BP 355 EP 359 PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GD885 UT WOS:A1991GD88500011 PM 1783003 ER PT J AU LESCH, KP AULAKH, CS TOLLIVER, TJ HILL, JL MURPHY, DL AF LESCH, KP AULAKH, CS TOLLIVER, TJ HILL, JL MURPHY, DL TI REGULATION OF G-PROTEINS BY CHRONIC ANTIDEPRESSANT DRUG-TREATMENT IN RAT-BRAIN - TRICYCLICS BUT NOT CLORGYLINE INCREASE G0-ALPHA SUBUNITS SO EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION LA English DT Note DE G-PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; TRICYCLIC ANTIDEPRESSANTS; CLORGYLINE AB The effect of long-term (3-week) administration of various antidepressant drugs on the steady-state concentrations of G protein alpha-subunits, G(s-alpha), G(i-alpha), and G(o-alpha), has been investigated in rat brain using an enzyme-linked immunosorbent assay. Tricyclic antidepressants and clorgyline decreased G(s-alpha) and, to a lesser extent, G(i-alpha) in several brain regions, while G(o-alpha) was increased by tricyclics but not clorgyline. We conclude that long-term treatment with antidepressant drugs exerts differential effects on G protein alpha-subunits, and that antidepressant efficacy may potentially be based on functional modifications of signal transduction. RP LESCH, KP (reprint author), NIMH,CTR CLIN,CLIN SCI LAB,10-3D41,BETHESDA,MD 20892, USA. RI Lesch, Klaus-Peter/J-4906-2013 OI Lesch, Klaus-Peter/0000-0001-8348-153X NR 11 TC 69 Z9 70 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0922-4106 J9 EUR J PHARM-MOLEC PH JI Eur. J. Pharmacol.-Molec. Pharmacol. Sect. PD AUG 14 PY 1991 VL 207 IS 4 BP 361 EP 364 PG 4 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GD885 UT WOS:A1991GD88500012 PM 1783004 ER PT J AU HOOFNAGLE, JH AF HOOFNAGLE, JH TI THROMBOCYTOPENIA DURING INTERFERON ALFA THERAPY SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter ID CHRONIC HEPATITIS; ALPHA-INTERFERON RP HOOFNAGLE, JH (reprint author), NIH,BETHESDA,MD 20892, USA. NR 4 TC 33 Z9 33 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD AUG 14 PY 1991 VL 266 IS 6 BP 849 EP 849 DI 10.1001/jama.266.6.849 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA GA073 UT WOS:A1991GA07300042 PM 1865526 ER PT J AU LAKSHMAN, MK SAYER, JM JERINA, DM AF LAKSHMAN, MK SAYER, JM JERINA, DM TI CHEMICAL SYNTHESIS OF A BAY-REGION POLYCYCLIC AROMATIC HYDROCARBON TETRAHYDROEPOXIDE DEOXYADENOSINE ADDUCT AND ITS SITE-SPECIFIC INCORPORATION INTO A DNA OLIGOMER SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Article ID DIOL-EPOXIDES; ABSOLUTE STEREOCHEMISTRY; NUCLEOSIDE ADDUCTS; BENZOPYRENE; ADENINE; DEOXYGUANOSINE; 1,2-EPOXIDES; CARCINOGEN; MUTATIONS; GUANINE AB The bay-region tetrahydrophenanthrene-3,4-epoxide represents a prototype for the carcinogenic bay-region diol epoxides of polycyclic aromatic hydrocarbons. We describe herein the chemical synthesis of the trans N-6 amino adduct of 2'-deoxyadenosine (dA) with the tetrahydroepoxide at the benzylic 4-position. The adduct was prepared by coupling of the trans C-4 aminolysis product of the epoxide with the 6-fluoro analogue of dA in which the sugar hydroxyl groups were protected as silyl ethers. The resulting pair of diastereomers, after chromatographic separation, were assigned absolute configurations through the use of optically pure epoxide of known configuration. Stereoisomerically identical trans adducts as well as the corresponding pair of diastereomers resulting from cis addition are formed on reaction of the racemic epoxide with 2'-deoxyadenosine 5'-phosphate. The (3S,4S)-diastereomer, after appropriate derivatization, has been incorporated in high yield into the deoxypentamer TpGpApGpT to document the utility of the nucleotide coupling as well as the blocking/deblocking procedures in the synthesis of a hydrocarbon-adducted oligomer. Synthetic accessibility of DNA containing single, specific adducts of carcinogenic bay-region diol epoxides will be of immense value to an understanding of how such modified sequences are enzymatically processed within the cell and how such processing results in cell transformation. C1 NIDDK,OXIDAT MECHANISMS SECT,BIOORGAN CHEM LAB,BETHESDA,MD 20892. NR 33 TC 54 Z9 54 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD AUG 14 PY 1991 VL 113 IS 17 BP 6589 EP 6594 DI 10.1021/ja00017a034 PG 6 WC Chemistry, Multidisciplinary SC Chemistry GA GB298 UT WOS:A1991GB29800034 ER PT J AU PETTIT, GR GAO, F HERALD, DL BLUMBERG, PM LEWIN, NE NIEMAN, RA AF PETTIT, GR GAO, F HERALD, DL BLUMBERG, PM LEWIN, NE NIEMAN, RA TI ANTINEOPLASTIC AGENTS .224. ISOLATION AND STRUCTURE OF NERISTATIN-1 SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Note ID SEPARATION C1 ARIZONA STATE UNIV,DEPT CHEM,TEMPE,AZ 85287. NCI,CELLULAR CARCINOGENIS & TUMOR PROMOT,BETHESDA,MD 20892. RP PETTIT, GR (reprint author), ARIZONA STATE UNIV,CANC RES INST,TEMPE,AZ 85287, USA. NR 14 TC 17 Z9 18 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD AUG 14 PY 1991 VL 113 IS 17 BP 6693 EP 6695 DI 10.1021/ja00017a062 PG 3 WC Chemistry, Multidisciplinary SC Chemistry GA GB298 UT WOS:A1991GB29800062 ER PT J AU MISCHAK, H BODENTEICH, A KOLCH, W GOODNIGHT, J HOFER, F MUSHINSKI, JF AF MISCHAK, H BODENTEICH, A KOLCH, W GOODNIGHT, J HOFER, F MUSHINSKI, JF TI MOUSE PROTEIN-KINASE C-DELTA, THE MAJOR ISOFORM EXPRESSED IN MOUSE HEMATOPOIETIC-CELLS - SEQUENCE OF THE CDNA, EXPRESSION PATTERNS, AND CHARACTERIZATION OF THE PROTEIN SO BIOCHEMISTRY LA English DT Article ID PHORBOL ESTERS; RAT-BRAIN; ACTIVATION; RECEPTOR; FAMILY; PURIFICATION; ONCOGENE; NPKC AB A complementary DNA (cDNA) of 2559 bp which encode all 674 amino acids of mouse protein kinase C-delta (PKC-delta) has been isolated from a cDNA library Prepared from ABPL-2, a mouse myeloid tumor. The library was screened with a partial PKC-delta cDNA clone that had been created by polymerase chain reaction (PCR) amplification of ABPL-2 RNA using primers that are conserved among all rat PKC isozymes. This approach proved to be a distinct improvement over screening with synthetic oligonucleotides. Similar sets of cDNAs prepared from other hemopoietic cell lines were screened with this PKC-delta cDNA and with probes for the other PKC isoforms. These experiments revealed that the major isoform of PKC expressed in hemopoietic cells is PKC-delta. PKC-delta protein was purified from ABPL-3, a mouse myeloid tumor which expressed principally the delta-isoform of PKC. The protein eluted from a hydroxylapatite column in the same position as PKC-beta and -epsilon would elute, if present. The kinase activity of purified PKC-delta showed strict dependence on the presence of phospholipids, but showed no activation by Ca2+. C1 NCI,GENET LAB,BLDG 37,ROOM 2B04,BETHESDA,MD 20892. INST BIOCHEM,A-1090 VIENNA,AUSTRIA. NIMH,BIOCHEM GENET LAB,WASHINGTON,DC 20032. NCI,FREDERICK CANC RES FACIL,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21701. RI Mischak, Harald/E-8685-2011 NR 22 TC 91 Z9 93 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD AUG 13 PY 1991 VL 30 IS 32 BP 7925 EP 7931 DI 10.1021/bi00246a008 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA940 UT WOS:A1991GA94000008 PM 1868068 ER PT J AU AIDA, K NEGISHI, M AF AIDA, K NEGISHI, M TI POSTTRANSCRIPTIONAL REGULATION OF COUMARIN 7-HYDROXYLASE INDUCTION BY XENOBIOTICS IN MOUSE-LIVER - MESSENGER-RNA STABILIZATION BY PYRAZOLE SO BIOCHEMISTRY LA English DT Article ID POST-TRANSCRIPTIONAL REGULATION; 3' UNTRANSLATED REGION; ELEMENT-BINDING-PROTEIN; TESTOSTERONE 16-ALPHA-HYDROXYLASE; RAT HEPATOCYTES; GENE FAMILY; STEROID 15-ALPHA-HYDROXYLASE; AFFINITY PURIFICATION; II P-45015-ALPHA; EXPRESSION AB The induction mechanism by pyrazole or phenobarbital of coumarin 7-hydroxylase (cytochrome P450coh) was investigated in DBA/2J male mice. The P450coh mRNA in the pyrazole-induced mice was increased gradually to a 20-fold higher level within 48 h, yet transcription of the P450coh gene was not affected. The half-life of P450coh mRNA, on the other hand, was at least 4-fold longer in the pyrazole-induced DBA/2J (approximately 6.0 h) than in control DBA/2J (approximately 1.5 h) male mice. The stabilization of P450coh mRNA, therefore, is the primary mechanism for the induction by pyrazole of coumarin 7-hydroxylase. Phenobarbital, on the other hand, regulates the induction either translationally or posttranslationally. This drug affected neither the P450coh mRNA nor the P450coh gene's transcription levels in the DBA/2J male mice, although Western blots showed approximately a 3-fold increase of the P450coh protein in the liver microsomes of the drug-treated mice. The results indicate, therefore, that both phenobarbital and pyrazole regulate the P450coh induction posttranscriptionally; the former inducer enhances the translational efficiency of P450coh mRNA or alters the degradation rate of P450coh apoprotein, while the latter stabilizes P450coh mRNA. C1 NIEHS,REPROD & DEV TOXICOL LAB,PHARMACOGENET SECT,RES TRIANGLE PK,NC 27709. NR 44 TC 49 Z9 52 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD AUG 13 PY 1991 VL 30 IS 32 BP 8041 EP 8045 DI 10.1021/bi00246a023 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA940 UT WOS:A1991GA94000023 PM 1868078 ER PT J AU KITADE, Y NAKATA, Y HIROTA, K MAKI, Y PABUCCUOGLU, A TORRENCE, PF AF KITADE, Y NAKATA, Y HIROTA, K MAKI, Y PABUCCUOGLU, A TORRENCE, PF TI 8-METHYLADENOSINE-SUBSTITUTED ANALOGS OF 2-5A - SYNTHESIS AND THEIR BIOLOGICAL-ACTIVITIES SO NUCLEIC ACIDS RESEARCH LA English DT Article ID CHEMICAL SYNTHESIS; BINDING; 2',5'-OLIGOADENYLATES; ACTIVATION; RNASE AB 8-Methyladenosine-substituted analogues of 2-5A, p5'A2'p5'A2'p5'(me8A), p5'A2'p5'(me8A)2'p5'(me8A), p5'(me8A)2'p5'(me8A)2'p5'(me8A), and p5'(me8A) 2'p5'A2'p5'A, were prepared via a modification of a lead ion-catalyzed ligation reaction. These 2-5A monophosphates were converted into the corresponding 5'-triphosphates. Substitution of an 8-methyladenosine residue at the third position (2'-terminus) of the oligonucleotides increased the stability to snake venom phosphodiesterase digestion. Both binding and activation of mouse liver 2-5A dependent ribonuclease (RNase L) by the various 8-methyladenosine-substituted 2-5A analogues were examined. Among the 8-methyladenosine-substituted trimer analogues, the analogues with 8-methyladenosine residing in the 2'-terminal position showed the strongest binding affinity and were several times more effective than 2-5A itself as an inhibitor of translation. C1 NIDDKD,MED CHEM LAB,BIOMED CHEM SECT,BETHESDA,MD 20892. RP KITADE, Y (reprint author), GIFU COLL PHARM,MED CHEM LAB,5-6-1 MITAHORA HIGASHI,GIFU 502,JAPAN. NR 21 TC 27 Z9 27 U1 2 U2 7 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 11 PY 1991 VL 19 IS 15 BP 4103 EP 4108 DI 10.1093/nar/19.15.4103 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB237 UT WOS:A1991GB23700008 PM 1714563 ER PT J AU CHAJUT, A YANIV, A AVIVI, L BARAM, I TRONICK, SR GAZIT, A AF CHAJUT, A YANIV, A AVIVI, L BARAM, I TRONICK, SR GAZIT, A TI A NOVEL-APPROACH FOR ESTABLISHING COMMON OR RANDOM INTEGRATION LOCI FOR RETROVIRAL GENOMES SO NUCLEIC ACIDS RESEARCH LA English DT Article ID INSITU C1 TEL AVIV UNIV,SACKLER SCH MED,DEPT HUMAN MICROBIOL,IL-69978 TEL AVIV,ISRAEL. NCI,LCMB,BETHESDA,MD 20892. TEL AVIV UNIV,SACKLER SCH MED,DEPT HUMAN GENET,TEL AVIV,ISRAEL. NR 7 TC 1 Z9 1 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 11 PY 1991 VL 19 IS 15 BP 4299 EP 4299 DI 10.1093/nar/19.15.4299 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB237 UT WOS:A1991GB23700046 PM 1870986 ER PT J AU POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR AF POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR TI TRINUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN MET-TRANSFER RNA-I GENE-1 (TRMI) SO NUCLEIC ACIDS RESEARCH LA English DT Article RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 4 TC 92 Z9 92 U1 1 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 11 PY 1991 VL 19 IS 15 BP 4306 EP 4306 DI 10.1093/nar/19.15.4306-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB237 UT WOS:A1991GB23700053 PM 1870993 ER PT J AU POLYMEROPOULOS, MH RATH, DS XIAO, H MERRIL, CR AF POLYMEROPOULOS, MH RATH, DS XIAO, H MERRIL, CR TI TETRANUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN COAGULATION FACTOR-XII A-SUBUNIT GENE (F13A1) SO NUCLEIC ACIDS RESEARCH LA English DT Article RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 4 TC 92 Z9 92 U1 1 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 11 PY 1991 VL 19 IS 15 BP 4306 EP 4306 DI 10.1093/nar/19.15.4306-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB237 UT WOS:A1991GB23700054 PM 1870993 ER PT J AU POLYMEROPOULOS, MH RATH, DS XIAO, H MERRIL, CR AF POLYMEROPOULOS, MH RATH, DS XIAO, H MERRIL, CR TI TRINUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN TRANSCRIPTION FACTOR-IID GENE SO NUCLEIC ACIDS RESEARCH LA English DT Article RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 3 TC 33 Z9 34 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD AUG 11 PY 1991 VL 19 IS 15 BP 4307 EP 4307 DI 10.1093/nar/19.15.4307 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB237 UT WOS:A1991GB23700055 PM 1870994 ER PT J AU GERFEN, CR AF GERFEN, CR TI SUBSTANCE-P (NEUROKININ-1) RECEPTOR MESSENGER-RNA IS SELECTIVELY EXPRESSED IN CHOLINERGIC NEURONS IN THE STRIATUM AND BASAL FOREBRAIN SO BRAIN RESEARCH LA English DT Note DE STRIATUM; TACHYKININ RECEPTOR; SUBSTANCE-P; STRIATONIGRAL; ACETYLCHOLINE ID MOLECULAR CHARACTERIZATION; DOPAMINE-D2 RECEPTORS; INSITU HYBRIDIZATION; CORRELATED LIGHT; NERVE-TERMINALS; FUNCTIONAL CDNA; RAT-BRAIN; NIGRA; LOCALIZATION; NEOSTRIATUM AB In the striatum substance P (neurokinin-1) receptor, mRNA is selectively localized in large neurons that also express mRNA encoding choline acetyltransferase (ChAT) by in situ hybridization histochemistry. Substance P receptor mRNA is also localized in ChAT mRNA-containing neurons in the medial septum and basal forebrain cell groups. Thus, in the rat forebrain the substance P receptor appears to be expressed selectively by cholinergic neurons. Striatal neurons that contain substance P also utilize gamma-aminobutyric acid (GABA) as a transmitter. These neurons make synaptic contact with striatal cholinergic neurons, which are shown here to express the substance P receptor, and with other GABAergic neurons in the striatum and substantia nigra, which express GABA receptors but not substance P receptors. This suggests that individual striatal neurons may differentially affect target neurons dependent on the receptors expressed by those target neurons. RP GERFEN, CR (reprint author), NIMH,DEPT CELL BIOL,BLDG 36,RM 2D-10,BETHESDA,MD 20892, USA. NR 32 TC 160 Z9 161 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD AUG 9 PY 1991 VL 556 IS 1 BP 165 EP 170 DI 10.1016/0006-8993(91)90563-B PG 6 WC Neurosciences SC Neurosciences & Neurology GA GD897 UT WOS:A1991GD89700024 PM 1718557 ER PT J AU RESNICK, NM MALOY, WL GUY, HR ZASLOFF, M AF RESNICK, NM MALOY, WL GUY, HR ZASLOFF, M TI A NOVEL ENDOPEPTIDASE FROM XENOPUS THAT RECOGNIZES ALPHA-HELICAL SECONDARY STRUCTURE SO CELL LA English DT Article ID EUKARYOTIC SIGNAL PEPTIDASE; CLEAVAGE SITE; SECRETORY GRANULES; HORMONE PRECURSORS; SINGLE ARGININE; LIPID BILAYERS; LAEVIS SKIN; SUBSTRATE; SEQUENCE; FORMS AB The magainin peptides of Xenopus laevis are broad-spectrum antimicrobial agents. Upon discharge from the skin glands, these basic, amphipathic peptides are each further processed at a single Xaa-Lys bond into half-peptides by a cosecreted protease. We describe the characterization and purification to homogeneity of this endopeptidase from Xenopus skin. The enzyme is a metalloprotease 110 kd in size. Analyses of substrate specificity revealed that the endopeptidase recognizes peptides that share the ability to adopt an amphipathic, alpha-helical motif composed of at least 12 residues, with one face strongly hydrophobic. Cleavage occurs on the amino side of a specific lysine that must be precisely positioned relative to the hydrophobic face of the alpha-helix. This enzyme, which we propose to call "magaininase," represents a novel class of endopeptidases that hydrolyzes peptides on the basis of specific secondary structure rather than primary amino acid sequence. C1 UNIV PENN,MOLEC BIOL GRAD GRP,PHILADELPHIA,PA 19104. MAGAININ SCI INC,PLYMOUTH MEETING,PA 19462. NCI,MATH BIOL LAB,BETHESDA,MD 20892. RP RESNICK, NM (reprint author), CHILDRENS HOSP PHILADELPHIA,DIV HUMAN GENET,PHILADELPHIA,PA 19104, USA. NR 52 TC 67 Z9 68 U1 1 U2 3 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD AUG 9 PY 1991 VL 66 IS 3 BP 541 EP 554 DI 10.1016/0092-8674(81)90017-9 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA GA941 UT WOS:A1991GA94100015 PM 1868549 ER PT J AU GRONENBORN, AM FILPULA, DR ESSIG, NZ ACHARI, A WHITLOW, M WINGFIELD, PT CLORE, GM AF GRONENBORN, AM FILPULA, DR ESSIG, NZ ACHARI, A WHITLOW, M WINGFIELD, PT CLORE, GM TI A NOVEL, HIGHLY STABLE FOLD OF THE IMMUNOGLOBULIN BINDING DOMAIN OF STREPTOCOCCAL PROTEIN-G SO SCIENCE LA English DT Article ID NUCLEAR MAGNETIC-RESONANCE; DISTANCE GEOMETRY; 3-DIMENSIONAL STRUCTURES; STAPHYLOCOCCUS-AUREUS; MOLECULAR-DYNAMICS; SPECTROSCOPY; CRYSTALLOGRAPHY; RESOLUTION; PROGRAM; CRAMBIN AB The high-resolution three-dimensional structure of a single immunoglobulin binding domain (BI, which comprises 56 residues including the NH2-terminal Met) of protein G from group G Streptococcus has been determined in solution by nuclear magnetic resonance spectroscopy on the basis of 1058 experimental restraints. The average atomic root-mean-square distribution about the mean coordinate positions is 0.27 angstrom (angstrom) for the backbone atoms, 0.65 angstrom for all atoms, and 0.39 angstrom for atoms excluding disordered surface side chains. The structure has no disulfide bridges and is composed of a four-stranded 13 sheet, on top of which lies a long helix. The central two strands (beta-1 and beta-4), comprising the NH2- and COOH-termini, are parallel, and the outer two strands (beta-2 and beta-3) are connected by the helix in a +3x crossover. This novel topology (-1, +3x, -1), coupled with an extensive hydrogen-bonding network and a tightly packed and buried hydrophobic core, is probably responsible for the extreme thermal stability of this small domain (reversible melting at 87-degrees-C). C1 GENEX CORP,GAITHERSBURG,MD 20877. NIH,PROT EXPRESS LAB,OFF DIRECTOR,BETHESDA,MD 20892. RP GRONENBORN, AM (reprint author), NIDDKD,CHEM PHYS LAB,BLDG 2,BETHESDA,MD 20892, USA. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 47 TC 586 Z9 590 U1 8 U2 62 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD AUG 9 PY 1991 VL 253 IS 5020 BP 657 EP 661 DI 10.1126/science.1871600 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GA304 UT WOS:A1991GA30400037 PM 1871600 ER PT J AU KINZLER, KW NILBERT, MC SU, LK VOGELSTEIN, B BRYAN, TM LEVY, DB SMITH, KJ PREISINGER, AC HEDGE, P MCKECHNIE, D FINNIEAR, R MARKHAM, A GROFFEN, J BOGUSKI, MS ALTSCHUL, SF HORII, A ANDO, H MIYOSHI, Y MIKI, Y NISHISHO, I NAKAMURA, Y AF KINZLER, KW NILBERT, MC SU, LK VOGELSTEIN, B BRYAN, TM LEVY, DB SMITH, KJ PREISINGER, AC HEDGE, P MCKECHNIE, D FINNIEAR, R MARKHAM, A GROFFEN, J BOGUSKI, MS ALTSCHUL, SF HORII, A ANDO, H MIYOSHI, Y MIKI, Y NISHISHO, I NAKAMURA, Y TI IDENTIFICATION OF FAP LOCUS GENES FROM CHROMOSOME-5Q21 SO SCIENCE LA English DT Article ID FAMILIAL ADENOMATOUS POLYPOSIS; COLORECTAL TUMORIGENESIS; PROTEIN; TUMORS; COLI; LOCALIZATION; CARCINOMA; SEQUENCE; PRODUCT; CANCER AB Recent studies suggest that one or more genes on chromosome 5q2l are important for the development of colorectal cancers, particularly those associated with familial adenomatous polyposis (F"). To facilitate the identification of genes from this locus, a portion of the region that is tightly linked to FAP was cloned. Six contiguous stretches of sequence (contigs) containing approximately 5.5 Mb of DNA were isolated. Subclones from these contigs were used to identify and position six genes, all of which were expressed in normal colonic mucosa. Two of these genes (APC and MCC) are likely to contribute to colorectal tumorigenesis. The MCC gene had previously been identified by virtue of its mutation in human colorectal tumors. The APC gene was identified in a contig initiated from the MCC gene and was found to encode an unusually large protein. These two closely spaced genes encode proteins predicted to contain coiled-coil regions. Both genes were also expressed in a wide variety of tissues. Further studies of MCC and APC and their potential interaction should prove useful for understanding colorectal neoplasia. C1 JOHNS HOPKINS UNIV, SCH MED, MOLEC GENET LAB, BALTIMORE, MD 21231 USA. ICI PLC, PHARMACEUT, MACCLESFIELD SK10 4TG, CHESHIRE, ENGLAND. CHILDRENS HOSP LOS ANGELES, DEPT PATHOL, LOS ANGELES, CA 90027 USA. NATL LIB MED, NATL CTR BIOTECHNOL INFORMAT, BETHESDA, MD 20894 USA. JAPANESE FDN CANC RES, INST CANC, DEPT BIOCHEM, TOKYO 170, JAPAN. RI Bryan, Tracy/B-8468-2014 FU NCI NIH HHS [CA06973, CA35494, CA44688] NR 59 TC 1777 Z9 1808 U1 2 U2 28 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD AUG 9 PY 1991 VL 253 IS 5020 BP 661 EP 665 DI 10.1126/science.1651562 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GA304 UT WOS:A1991GA30400038 PM 1651562 ER PT J AU MCCLURKIN, JW OPTICAN, LM RICHMOND, BJ GAWNE, TJ AF MCCLURKIN, JW OPTICAN, LM RICHMOND, BJ GAWNE, TJ TI CONCURRENT PROCESSING AND COMPLEXITY OF TEMPORALLY ENCODED NEURONAL MESSAGES IN VISUAL-PERCEPTION SO SCIENCE LA English DT Article ID TWO-DIMENSIONAL PATTERNS; SINGLE UNITS; CORTEX; STREAMS AB The intrinsic neuronal code that carries visual information and the perceptual mechanism for decoding that information are not known. However, multivariate statistics and information theory show that neurons in four visual areas simultaneously carry multiple, stimulus-related messages by utilizing multiplexed temporal codes. The complexity of these temporal messages increases progressively across the visual system, yet the temporal codes overlap in time. Thus, visual perception may depend on the concurrent processing of multiplexed temporal messages from all visual areas. C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. RP MCCLURKIN, JW (reprint author), NEI,SENSORIMOTOR RES LAB,BETHESDA,MD 20892, USA. NR 14 TC 93 Z9 93 U1 0 U2 3 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD AUG 9 PY 1991 VL 253 IS 5020 BP 675 EP 677 DI 10.1126/science.1908118 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA GA304 UT WOS:A1991GA30400042 PM 1908118 ER PT J AU PATTON, L SHIP, J WELLNER, R AF PATTON, L SHIP, J WELLNER, R TI N-(6-AMINOHEXYL)-5-CHLORO-1-NAPHTHALENESULFONAMIDE (W7) STIMULATION OF K+ TRANSPORT IN A HUMAN SALIVARY EPITHELIAL-CELL LINE SO BIOCHEMICAL PHARMACOLOGY LA English DT Article ID POTASSIUM CHANNELS; CALMODULIN ANTAGONISTS; KINASE-C; MEMBRANE; MOBILIZATION; CONDUCTANCE; MODULATION; VESICLES; GLAND; CA-2+ AB Treatment of a human salivary epithelial cell line, HSG-PA, with the calmodulin antagonist N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W7; 20-70-mu-M) increased Rb-86 (K+) influx and efflux in a manner similar to that resulting from muscarinic (carbachol; Cch) or calcium ionophore (A23187) stimulation. Unlike the Cch or A23187 responses, the W7 responses were not blocked by 0.1 mM atropine (muscarinic antagonist) or phorbol-12-myristate-13-acetate (0.1-mu-M). Like Cch- or A23187-stimulated Rb-86 fluxes, W7-stimulated Rb-86 fluxes were substantially blocked by the K+ channel inhibitors quinine (0.25 mM) and scorpion venom-containing charybdotoxin (33-mu-g/mL), while 5 mM tetraethylammonium chloride (K+ channel blocker), furosemide (0.1 mM; Na+, K+, 2Cl- co-transport inhibitor) and ouabain (10-mu-M; Na+, K+-ATPase inhibitor) were ineffective. Purified charybdotoxin (10 nM) also blocked W7-stimulated Rb-86 influx, as well as Rb-86 influx stimulated by Cch or A23187. Although Quin 2 fluorescence measurements indicated that W7 increased free intracellular Ca2+ concentration ([Ca2+]i), the magnitude of the increase appeared to be insufficient to solely account for the W7-stimulated increases in Rb-86 fluxes (i.e. K+ channel activity). Ca2+ was involved in the W7 response, however, as lack of Ca2+ in the incubation medium reduced the W7-stimulated increases in Rb-86 influx and efflux. Taken together, our results suggest that W7 increased K+ fluxes in HSG-PA cells by interacting, directly or indirectly, with the K+ transport machinery (K+ channels) in a manner different from that observed during muscarinic stimulation, and also in a manner not accounted for solely by the formation of a typical muscarinic- or calcium ionophore-generated calcium signal. C1 NIDR, CLIN INVEST & PATIENT CARE BRANCH, BETHESDA, MD 20892 USA. USA, MED RES INST INFECT DIS, DIV PATHOPHYSIOL, FREDERICK, MD 21702 USA. OI Patton, Lauren/0000-0002-8253-4588 NR 35 TC 2 Z9 2 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0006-2952 EI 1873-2968 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD AUG 8 PY 1991 VL 42 IS 5 BP 1039 EP 1044 DI 10.1016/0006-2952(91)90286-E PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GC812 UT WOS:A1991GC81200011 PM 1714731 ER PT J AU JABLON, S BOICE, JD AF JABLON, S BOICE, JD TI PUBLIC-HEALTH EFFECTS OF OCCUPATIONAL AND ENVIRONMENTAL RADIATION EXPOSURE - REPLY SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter RP JABLON, S (reprint author), NCI,RADIAT EPIDEMIOL BRANCH,BETHESDA,MD 20892, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD AUG 7 PY 1991 VL 266 IS 5 BP 655 EP 655 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA FZ140 UT WOS:A1991FZ14000016 ER PT J AU FLEG, JL AF FLEG, JL TI POTENTIAL COMPLICATIONS OF HIGH-DOSE EPINEPHRINE SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter ID DECREASE RP FLEG, JL (reprint author), NIH,CARDIOVASC SCI LAB,BALTIMORE,MD 21224, USA. NR 6 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD AUG 7 PY 1991 VL 266 IS 5 BP 656 EP 656 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA FZ140 UT WOS:A1991FZ14000018 ER PT J AU SHIELDS, PG HARRIS, CC AF SHIELDS, PG HARRIS, CC TI MOLECULAR EPIDEMIOLOGY AND THE GENETICS OF ENVIRONMENTAL CANCER SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID WHITE BLOOD-CELLS; L-MYC GENE; FRAGMENT-LENGTH-POLYMORPHISM; HYDROCARBON-DNA ADDUCTS; SISTER CHROMATID EXCHANGE; N-NITROSAMINO ACIDS; COKE-OVEN WORKERS; LOW-RISK AREAS; LUNG-CANCER; HEMOGLOBIN ADDUCTS AB Environmental, occupational, and recreational exposures to carcinogens contribute to cancer risk in humans. Cancer formation is a multistage process involving tumor initiation, promotion, conversion, and progression. Carcinogens can affect any of these stages through genetic and epigenetic mechanisms. The association of a suspected carcinogenic exposure and cancer risk can be studied in populations with classic epidemiologic techniques. However, these techniques are not applicable to the assessment of risk in individuals. Molecular epidemiology, in contrast, is a field that integrates molecular biology, in vitro and in vivo laboratory models, biochemistry, and epidemiology to infer individual cancer risk. Carcinogen-macromolecular adduct levels, and somatic cell mutations can be measured to determine the biologically effective dose of a carcinogen. Molecular epidemiology also explores host cancer susceptibilities, such as carcinogen metabolic activation, DNA repair, endogenous mutation rates, and inheritance of mutated tumor suppressor genes. Substantial interindividual variation for each of these biologic end points has been shown and, therefore, highlights the need for assessing cancer risk on an individual basis. Given the pace of the last decade, it is feasible that the next 10 years will allow molecular epidemiologists to develop a cancer-risk profile for an individual that includes assessment of a number of factors. This will help focus preventive strategies and strengthen quantitative risk assessments. C1 NCI,HUMAN CARCINOGENESIS LAB,BLDG 37,ROOM 2C01,BETHESDA,MD 20892. RI Shields, Peter/I-1644-2012 NR 96 TC 160 Z9 161 U1 0 U2 2 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD AUG 7 PY 1991 VL 266 IS 5 BP 681 EP 687 DI 10.1001/jama.266.5.681 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA FZ140 UT WOS:A1991FZ14000027 PM 2072479 ER PT J AU HOLM, LE HALL, P WIKLUND, K LUNDELL, G BERG, G BJELKENGREN, G CEDERQUIST, E ERICSSON, UB HALLQUIST, A LARSSON, LG LIDBERG, M LINDBERG, S TENNVALL, J WICKLUND, H BOICE, JD AF HOLM, LE HALL, P WIKLUND, K LUNDELL, G BERG, G BJELKENGREN, G CEDERQUIST, E ERICSSON, UB HALLQUIST, A LARSSON, LG LIDBERG, M LINDBERG, S TENNVALL, J WICKLUND, H BOICE, JD TI CANCER RISK AFTER I-131 THERAPY FOR HYPERTHYROIDISM SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID RETROSPECTIVE COHORT; THYROID-CANCER; BOMB SURVIVORS; I-131 THERAPY; BREAST-CANCER; FOLLOW-UP; RADIATION; MORTALITY; WOMEN; SITE AB Cancer incidence was studied in 10 552 patients (mean age, 57 years) who received I-131 therapy (mean dose, 506 MBq) for hyperthyroidism between 1950 and 1975. Follow-up on these patients was continued for an average of 15 years. Record linkage with the Swedish Cancer Register for the period 1958-1985 identified 1543 cancers occurring 1 year or more after I-131 treatment, and the standardized incidence ratio (SIR) was 1.06 (95% confidence interval = 1.01-1.11). Significantly increased SIRs were observed for cancers of the lung (SIR = 1.32; n = 105) and kidney (SIR = 1.39; n = 66). Among 10-year survivors, significantly elevated risks were seen for cancers of the stomach (SIR = 1.33; n = 58), kidney (SIR = 1.51; n = 37), and brain (SIR = 1.63; n = 30). Only the risk for stomach cancer, however, increased over time (P < .05) and with increasing activity administered (P = not significant). The risk for malignant lymphoma was significantly below expectation (SIR = 0.53; n = 11). Overall cancer risk did not increase with administered I-131 dose or with time since exposure. The absence of any increase in leukemia adds further support to the view that a radiation dose delivered gradually over time is less carcinogenic than the same total dose received over a short time. Only for stomach cancer was a possible radiogenic excess suggested. C1 KAROLINSKA HOSP,RADIUMHEMMET,DEPT GEN ONCOL,S-10401 STOCKHOLM 60,SWEDEN. KAROLINSKA HOSP,RADIUMHEMMET,DEPT CANC EPIDEMIOL,S-10401 STOCKHOLM 60,SWEDEN. SAHLGRENS UNIV HOSP,DEPT GEN ONCOL,S-41345 GOTHENBURG,SWEDEN. SAHLGRENS UNIV HOSP,DIV NUCL MED,S-41345 GOTHENBURG,SWEDEN. MALMO GEN HOSP,DEPT GEN ONCOL,S-21401 MALMO,SWEDEN. MALMO GEN HOSP,DEPT INTERNAL MED,S-21401 MALMO,SWEDEN. UNIV LUND HOSP,DEPT GEN ONCOL,S-22185 LUND,SWEDEN. UMEA UNIV HOSP,DEPT GEN ONCOL,S-90185 UMEA,SWEDEN. SODER SJUKHUSET,DEPT HOSP PHYS,STOCKHOLM,SWEDEN. UNIV HOSP UPPSALA,DEPT GEN ONCOL,UPPSALA,SWEDEN. NCI,DIV CANC ETIOL,EPIDEMIOL & BIOSTAT PROGRAM,BETHESDA,MD 20892. RP HOLM, LE (reprint author), KAROLINSKA HOSP,RADIUMHEMMET,DEPT CANC PREVENT,S-10401 STOCKHOLM 60,SWEDEN. RI Tennvall, Jan/F-8760-2014 FU NCI NIH HHS [N01-CP-51034] NR 25 TC 190 Z9 199 U1 1 U2 4 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD AUG 7 PY 1991 VL 83 IS 15 BP 1072 EP 1077 DI 10.1093/jnci/83.15.1072 PG 6 WC Oncology SC Oncology GA FZ503 UT WOS:A1991FZ50300009 PM 1875414 ER PT J AU CAUGHEY, BW DONG, A BHAT, KS ERNST, D HAYES, SF CAUGHEY, WS AF CAUGHEY, BW DONG, A BHAT, KS ERNST, D HAYES, SF CAUGHEY, WS TI SECONDARY STRUCTURE-ANALYSIS OF THE SCRAPIE-ASSOCIATED PROTEIN PRP 27-30 IN WATER BY INFRARED-SPECTROSCOPY SO BIOCHEMISTRY LA English DT Article ID CREUTZFELDT-JAKOB DISEASE; GERSTMANN-STRAUSSLER SYNDROME; CHAIN PLEATED SHEET; AMIDE-ONE VIBRATION; PRION PROTEIN; INCUBATION PERIOD; MOUSE-BRAIN; RESONANCE INTERACTION; POLYACRYLAMIDE GELS; AMYLOID PLAQUES AB A protease-resistant form of the protein PrP (PrP-res) accumulates in tissues of mammals infected with scrapie, Creutzfeldt-Jakob disease, and related transmissible neurodegenerative diseases. This abnormal form of PrP can aggregate into insoluble amyloid-like fibrils and plaques and has been identified as the major component of brain fractions enriched for scrapie infectivity. Using a recently developed technique in Fourier transform infrared spectroscopy which allows protein conformational analysis in aqueous media, we have studied the secondary structure of the proteinase K resistant core of PrP-res (PrP-res 27-30) as it exists in highly infectious fibril preparations. Second-derivative analysis of the infrared spectra has enabled us to quantitate the relative amounts of different secondary structures in the PrP-res aggregates. The analysis indicated that PrP-res 27-30 is predominantly composed of beta-sheet (47%), which is consistent with its amyloid-like properties. In addition, significant amounts of turn (31%) and alpha-helix (17%) were identified, indicating that amyloid-like fibrils need not be exclusively beta-sheet. The infrared-based secondary structure compositions were then used as constraints to improve the theoretical localization of the secondary structures within PrP-res 27-30. C1 COLORADO STATE UNIV,DEPT BIOCHEM,FT COLLINS,CO 80523. RP CAUGHEY, BW (reprint author), NIAID,ROCKY MT LABS,HAMILTON,MT 59840, USA. FU NHLBI NIH HHS [HL-15980] NR 70 TC 678 Z9 692 U1 3 U2 36 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD AUG 6 PY 1991 VL 30 IS 31 BP 7672 EP 7680 DI 10.1021/bi00245a003 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FZ965 UT WOS:A1991FZ96500003 PM 1678278 ER PT J AU FUNNELL, BE AF FUNNELL, BE TI THE P1 PLASMID PARTITION COMPLEX AT PARS - THE INFLUENCE OF ESCHERICHIA-COLI INTEGRATION HOST FACTOR AND OF SUBSTRATE TOPOLOGY SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID SITE-SPECIFIC RECOMBINATION; UNIT-COPY MINIPLASMIDS; CENTROMERE-LIKE SITE; BINDING-SITES; DAUGHTER CELLS; DNA; PROTEIN; REGION; REPLICATION; GENE AB The P1 ParB protein is required for active partition and thus stable inheritance of the plasmid prophage. ParB and the Escherichia coli protein integration host factor (IHF) participate in the assembly of a partition complex at the centromere-like site parS. In this report the role of IHF in the formation of the partition complex has been explored. First, ParB protein was purified for these studies, which revealed that ParB forms a dimer in solution. Next, the IHF binding site was mapped to a 29-base pair region within parS, including the sequence TAACTGACTGTTT (which differs from the IHF consensus in two positions). IHF induced a strong bend in the DNA at its binding site. Versions of parS which have lost or damaged the IHF binding site bound ParB with greatly reduced affinity in vitro and in vivo. Measurements of binding constants showed that IHF increased ParB affinity for the wild-type parS site by about 10,000-fold. Finally, DNA super-coiling improved ParB binding in the presence of IHF but not in its absence. These observations led to the proposal that IHF and superhelicity assist ParB by promoting its precise positioning at parS, a spatial arrangement that results in a high affinity of ParB for parS. C1 NCI,BIOCHEM LAB,BETHESDA,MD 20892. NR 47 TC 70 Z9 70 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 5 PY 1991 VL 266 IS 22 BP 14328 EP 14337 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FZ351 UT WOS:A1991FZ35100036 PM 1860842 ER PT J AU FISHER, LW HEEGAARD, AM VETTER, U VOGEL, W JUST, W TERMINE, JD YOUNG, MF AF FISHER, LW HEEGAARD, AM VETTER, U VOGEL, W JUST, W TERMINE, JD YOUNG, MF TI HUMAN BIGLYCAN GENE - PUTATIVE PROMOTER, INTRON-EXON JUNCTIONS, AND CHROMOSOMAL LOCALIZATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID SMALL PROTEOGLYCAN-I; AMINO-ACID-SEQUENCE; OSTEOINDUCTIVE FACTOR; CORE PROTEIN; BONE; DECORIN; LEUCINE; YEAST; PURIFICATION; DROSOPHILA AB Biglycan (PG-I, DS-PG1, PG-S1) is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin (PG-II, PG-S2, DS-PG2, or PG-40) and fibromodulin. The core protein is made up predominantly of a series of 11 tandem repeats that appear to have been used throughout evolution for protein-protein, protein-cell, or cell-cell interactions. The function of biglycan is unclear at this time, but it has been shown to bind transforming growth factor-beta in vitro. We have cloned and partially sequenced the approximately 8-kilobase pair human biglycan gene. The gene consists of eight exons including one in the sequence that encodes the 5'-untranslated region of the mRNA. The first and seventh introns are approximately 1 kilobase pair, while the remainder are shorter. With the exception of the first two introns, all of the introns are spread throughout the hydrophobic repeat domain. The 500-base pair 5' to the start of transcription contains several elements that strongly suggest that it contains a significant amount of the gene promoter. The elements include one AP2 and five SP1 consensus sequences. Like in many other genes, the biglycan gene promoter lacks both a CAAT and TATA box but is rich in GC content. Using H-3-labeled cDNA and in situ hybridization and autoradiography of human chromosomes, the human gene was localized to the end of the long arm of the X chromosome (Xq27-ter). The relationship of biglycan to a number of other proteins containing the leucine-rich repeats is discussed with respect to homologies of cysteine regions immediately adjacent to the repeat sequences. C1 UNIV ULM,KLIN GENET ABT,W-7900 ULM,GERMANY. RP FISHER, LW (reprint author), NIDR,BONE RES BRANCH,RM 106,BLDG 30,BETHESDA,MD 20892, USA. NR 36 TC 117 Z9 118 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 5 PY 1991 VL 266 IS 22 BP 14371 EP 14377 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FZ351 UT WOS:A1991FZ35100042 PM 1860845 ER PT J AU THOMPSON, J NGUYEN, NY SACKETT, DL DONKERSLOOT, JA AF THOMPSON, J NGUYEN, NY SACKETT, DL DONKERSLOOT, JA TI TRANSPOSON-ENCODED SUCROSE METABOLISM IN LACTOCOCCUS-LACTIS - PURIFICATION OF SUCROSE-6-PHOSPHATE HYDROLASE AND GENETIC-LINKAGE TO N5-(L-1-CARBOXYETHYL)-L-ORNITHINE SYNTHASE IN STRAIN K1 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID STREPTOCOCCUS-LACTIS; PHOSPHOTRANSFERASE SYSTEM; NISIN PRODUCTION; NUCLEOTIDE-SEQUENCE; FERMENTING ABILITY; 6-PHOSPHATE HYDROLASE; NADP+ OXIDOREDUCTASE; ANTIBIOTIC NISIN; SUCRASE GENE; MUTANS AB Sucrose-6-phosphate hydrolase from Lactococcus lactis subsp. lactis K1-23 (formerly Streptococcus lactis K1-23) has been purified 600-fold to electrophoretic homogeneity. Purification of the enzyme was achieved by DEAE-Sephacel, phosphocellulose P-11, and gel exclusion (Ultrogel AcA 54) chromatography. The purified enzyme (specific activity 31 units/mg) catalyzed the hydrolysis of both 6-O-phosphoryl-alpha-D-glucopyranosyl-1, 2-beta-D-fructofuranoside (sucrose 6-phosphate) and sucrose (K(m) = 0.1 and 100 mM, respectively). Ultracentrifugal analysis of sucrose-6-phosphate hydrolase indicated an M(r) = 52,200. The purified enzyme migrated as a single protein during sodium dodecyl sulfate-polyacrylamide gel electrophoresis (M(r) = 52,000). However, four distinct polypeptides were detected by analytical electrofocusing, and all four species hydrolyzed sucrose and sucrose 6-phosphate. The amino acid composition of sucrose-6-phosphate hydrolase, and the sequence of the first 12 amino acids from the NH2 terminus, have been determined. Hybridization studies with oligonucleotide probes show that the genes for sucrose-6-phosphate hydrolase (scrB), Enzyme II(Scr) of the phosphoenolpyruvate-dependent sucrose:phosphotransferase system (scrA), and N5-(carboxyethyl) ornithine synthase (ceo) are encoded by the same approximately 20-kilobase EcoRI fragment. This fragment is part of a large transposon Tn5306 that also encodes the nisin precursor gene, spaN, and IS904. In L. lactis ATCC 11454, spaN, IS904, scrA, and scrB (but not ceo) are encoded on a related transposon, Tn5307. C1 NIDDKD, BETHESDA, MD 20892 USA. US FDA, CTR BIOL EVALUAT & RES, DIV CYTOKINE BIOL, BETHESDA, MD 20892 USA. RP THOMPSON, J (reprint author), NIDR, MICROBIAL ECOL LAB, BLDG 30, RM 528, BETHESDA, MD 20892 USA. NR 50 TC 33 Z9 34 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 5 PY 1991 VL 266 IS 22 BP 14573 EP 14579 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FZ351 UT WOS:A1991FZ35100072 PM 1650362 ER PT J AU COWART, M BENKOVIC, SJ NASH, HA AF COWART, M BENKOVIC, SJ NASH, HA TI BEHAVIOR OF A CROSS-LINKED ATTACHMENT SITE - TESTING THE ROLE OF BRANCH MIGRATION IN SITE-SPECIFIC RECOMBINATION SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE BACTERIOPHAGE LAMBDA; SITE-SPECIFIC RECOMBINATION; BRANCH MIGRATION; CROSS-LINKING; HOLLIDAY JUNCTION ID LAMBDA INT GENE; BACTERIOPHAGE-LAMBDA; PHAGE-LAMBDA; ESCHERICHIA-COLI; DNA-SEQUENCE; HOMOLOGY; PROTEIN; SYNAPSIS; ENZYMES; MUTANTS C1 PENN STATE UNIV,DEPT CHEM,UNIVERSITY PK,PA 16802. NIMH,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 30 TC 12 Z9 12 U1 0 U2 1 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD AUG 5 PY 1991 VL 220 IS 3 BP 621 EP 629 DI 10.1016/0022-2836(91)90105-F PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA302 UT WOS:A1991GA30200010 PM 1831237 ER PT J AU HOUSSET, D KIM, KS FUCHS, J WOODWARD, C WLODAWER, A AF HOUSSET, D KIM, KS FUCHS, J WOODWARD, C WLODAWER, A TI CRYSTAL-STRUCTURE OF A Y35G MUTANT OF BOVINE PANCREATIC TRYPSIN-INHIBITOR SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE BPTI; CRYSTAL STRUCTURE; MUTATION; CONFORMATIONAL CHANGE; MOLECULAR REPLACEMENT ID LEAST-SQUARES REFINEMENT; MACROMOLECULAR STRUCTURES; MOLECULAR-DYNAMICS; ALZHEIMERS-DISEASE; PROTEIN STRUCTURES; DISULFIDE-BRIDGE; ESCHERICHIA-COLI; RESOLUTION; PRECURSOR; DOMAIN C1 NCI,FREDERICK CANC RES & DEV CTR,MACROMOLEC STRUCT LAB,ABL,BASIC RES PROGRAM,FREDERICK,MD 21702. UNIV MINNESOTA,DEPT BIOCHEM,ST PAUL,MN 55108. FU NIGMS NIH HHS [GM26242]; PHS HHS [N01-C0-74101] NR 45 TC 60 Z9 61 U1 0 U2 1 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD AUG 5 PY 1991 VL 220 IS 3 BP 757 EP 770 DI 10.1016/0022-2836(91)90115-M PG 14 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA302 UT WOS:A1991GA30200020 PM 1714504 ER PT J AU LOVINGER, DM AF LOVINGER, DM TI TRANS-1-AMINOCYCLOPENTANE-1,3-DICARBOXYLIC ACID (TERT-ACPD) DECREASES SYNAPTIC EXCITATION IN RAT STRIATAL SLICES THROUGH A PRESYNAPTIC ACTION SO NEUROSCIENCE LETTERS LA English DT Article DE TERT-ACPD; SYNAPTIC EXCITATION; RAT STRIATUM; PRESYNAPTIC ACTION; GLUTAMATE; CAUDATE/PUTAMEN; AUTORECEPTOR; PATCH SLICE ID HIPPOCAMPAL-NEURONS; GLUTAMATE RECEPTORS; FAMILY AB Excitatory synaptic transmission was decreased in the presence of trans-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) during whole-cell recordings from striatal slices. This effect of t-ACPD increased in a concentration-dependent manner at drug concentrations from 5-100-mu-M. Decreased transmission appeared to result from a presynaptic effect as indicated by the observations that: (1) t-ACPD decreased the amplitude of excitatory postsynaptic potentials (EPSPs) mediated by either quisqualate alpha-amino-3-hydroxy-5-methyl-4-isoxazole-proprianate (AMPA) or N-methyl-D-aspartate (NMDA) receptors, but did not block responses to agonist application; indicating that t-ACPD is not a glutamate receptor antagonist; (2) t-ACPD decreased transmission in the absence of changes in postsynaptic cell properties. The t-ACPD-activated receptor appears to differ from previously identified presynaptic receptors. These findings indicate that t-ACPD activates a presynaptic receptor; most likely a glutamate receptor. C1 NIAAA,PHYSIOL & PHARMACOL STUDIES,ELECTROPHYSIOL SECT,ROCKVILLE,MD 20852. NR 17 TC 136 Z9 137 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD AUG 5 PY 1991 VL 129 IS 1 BP 17 EP 21 DI 10.1016/0304-3940(91)90710-B PG 5 WC Neurosciences SC Neurosciences & Neurology GA GA847 UT WOS:A1991GA84700004 PM 1656337 ER PT J AU LI, R WING, LL SHEN, Y WYATT, RJ KIRCH, DG CHUANG, DM AF LI, R WING, LL SHEN, Y WYATT, RJ KIRCH, DG CHUANG, DM TI CHRONIC HALOPERIDOL TREATMENT ATTENUATES RECEPTOR-MEDIATED PHOSPHOINOSITIDE TURNOVER IN RAT-BRAIN SLICES SO NEUROSCIENCE LETTERS LA English DT Article DE HALOPERIDOL; CARBACHOL; NOREPINEPHRINE; PHOSPHOINOSITIDE TURNOVER; FRONTAL CORTEX; HIPPOCAMPUS; STRIATUM ID INOSITOL PHOSPHOLIPID HYDROLYSIS; ANTERIOR-PITUITARY CELLS; LONG-TERM HALOPERIDOL; NEUROTRANSMITTER RECEPTORS; STRIATAL SLICES; DOPAMINE; AGONIST; CORTEX; ACETYLCHOLINE AB The long-term effects of haloperidol on phosphoinositide turnover in rat brain slices were investigated. Continuous treatment with haloperidol decanoate (21 mg/kg I.M. biweekly for 6 weeks) significantly attenuated carbachol- and norepinephrine (NE)-induced inositol phosphate accumulation in rat frontal cortex and hippocampus. In the striatum, the haloperidol treatment also significantly decreased carbachol-stimulated inositol phosphate level but did not significantly affect NE-sensitive phosphoinositide turnover. These effects were not observed in rats treated with a single dose of haloperidol (1.5 mg/kg). Basal levels of inositol phosphate in these 3 brain regions did not change following continuous or single haloperidol doses. C1 NIMH,BIOL PSYCHIAT BRANCH,BLDG 10,RM 3N212,BETHESDA,MD 20892. ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. NR 18 TC 9 Z9 9 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD AUG 5 PY 1991 VL 129 IS 1 BP 81 EP 85 DI 10.1016/0304-3940(91)90725-9 PG 5 WC Neurosciences SC Neurosciences & Neurology GA GA847 UT WOS:A1991GA84700019 PM 1656342 ER PT J AU GRAHAM, NMH ZEGER, SL PARK, LP PHAIR, JP DETELS, R VERMUND, SH HO, MT SAAH, AJ AF GRAHAM, NMH ZEGER, SL PARK, LP PHAIR, JP DETELS, R VERMUND, SH HO, MT SAAH, AJ TI EFFECT OF ZIDOVUDINE AND PNEUMOCYSTIS-CARINII PNEUMONIA PROPHYLAXIS ON PROGRESSION OF HIV-1 INFECTION TO AIDS SO LANCET LA English DT Article ID IMMUNODEFICIENCY-VIRUS INFECTION; PLACEBO-CONTROLLED TRIAL; DOUBLE-BLIND; EFFICACY; SULFAMETHOXAZOLE; PENTAMIDINE; SAFETY; AZT AB Although used widely, the effectiveness of zidovudine therapy and primary prophylaxis for Pneumocystis carinii pneumonia (PCP) in HIV-1-infected individuals, has not been assessed in a large cohort. We have done an observational study between October, 1986, and October, 1990, of a cohort of 2145 HIV-1-seropositive men and 371 who seroconverted during the study. A Markov chain transitional analysis was used to examine the effect of zidovudine and PCP prophylaxis on the probability of progression of HIV-1 infection to AIDS (after 6,12,18, and 24 months) after follow-up visits categorised into one of six disease states. The six starting states were based on CD4+ lymphocyte counts and the presence of HIV-related symptoms. Use of pre-AIDS zidovudine and PCP prophylaxis was associated with significant reductions in rates of progression to AIDS at 6,12,18, and 24 months for participants starting with less than 350 CD4+ lymphocytes/mu-l. For those starting with 350 or more CD4+ lymphocytes/mu-l, non-significant protective trends were seen during 12, 18, and 24 month intervals. In multivariate log-linear models virtually all the treatment effect was due to zidovudine. However, after adjusting for the effects of zidovudine, PCP prophylaxis reduced significantly the probability of progression to a first episode of PCP during 6,12,18, and 24 month intervals. This study suggests that early primary PCP prophylaxis is effective in preventing first episodes of PCP, and that the efficacy of zidovudine demonstrated in clinical trials can be translated to the population level. C1 JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT BIOSTAT,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT MED,BALTIMORE,MD 21205. NORTHWESTERN UNIV,HOWARD BROWN MEM CLIN,CHICAGO,IL 60611. NORTHWESTERN UNIV,DEPT MED,CHICAGO,IL 60611. UNIV CALIF LOS ANGELES,SCH PUBL HLTH,DEPT EPIDEMIOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. NIAID,DIV AIDS,BETHESDA,MD 20892. UNIV PITTSBURGH,SCH PUBL HLTH,DEPT INFECT DIS & MICROBIOL,PITTSBURGH,PA 15260. RP GRAHAM, NMH (reprint author), JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT EPIDEMIOL,624 N BROADWAY,BALTIMORE,MD 21205, USA. OI Vermund, Sten/0000-0001-7289-8698 FU PHS HHS [N0-A1-32535, N01-A1-72634, N01-A1-72676] NR 18 TC 162 Z9 162 U1 0 U2 1 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD AUG 3 PY 1991 VL 338 IS 8762 BP 265 EP 269 DI 10.1016/0140-6736(91)90414-K PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA FZ565 UT WOS:A1991FZ56500001 PM 1677108 ER PT J AU FLATEN, TP GARRUTO, RM AF FLATEN, TP GARRUTO, RM TI DESFERRIOXAMINE FOR ALZHEIMERS-DISEASE SO LANCET LA English DT Letter ID DRINKING-WATER; ALUMINUM RP FLATEN, TP (reprint author), NIH,CENT NERVOUS SYST STUDIES LAB,BETHESDA,MD 20892, USA. RI Flaten, Trond Peder/I-3756-2013 NR 7 TC 6 Z9 6 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD AUG 3 PY 1991 VL 338 IS 8762 BP 324 EP 325 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA FZ565 UT WOS:A1991FZ56500059 ER PT J AU JUCKER, M KLEINMAN, HK HOHMANN, CF ORDY, JM INGRAM, DK AF JUCKER, M KLEINMAN, HK HOHMANN, CF ORDY, JM INGRAM, DK TI DISTINCT IMMUNOREACTIVITY TO 110KDA LAMININ-BINDING PROTEIN IN ADULT AND LESIONED RAT FOREBRAIN SO BRAIN RESEARCH LA English DT Article DE LAMININ-BINDING PROTEIN; SYNTHETIC LAMININ-DERIVED PEPTIDE PA22-2; NEUROTROPHIC FACTOR; IMMUNOCYTOCHEMISTRY; STAB WOUND; ISCHEMIC LESION; REACTIVE ASTROCYTE ID CENTRAL-NERVOUS-SYSTEM; NEURITE OUTGROWTH; NEUROMUSCULAR-JUNCTION; SYNTHETIC PEPTIDE; GROWTH-FACTOR; A-CHAIN; RECEPTORS; NEURONS; INJURY; BRAIN AB A phosphorylated, almost-equal-to 110 kDa laminin-binding protein (110 kDa LBP) from mouse brain has been previously identified. This protein recognizes a neurite-outgrowth promoting 19-amino acid synthetic peptide (PA22-2) derived from the laminin A chain. In the present study, an antibody against the 110 kDa LBP was used to localize immunoreactivity in the normal adult rat brain and also following a stab wound and ischemic lesion. Immunoreactive cells were found in layers II/III and V of the cerebral cortex and within apical dendrites of pyramidal neurons. Specific immunoreactivity was also found in the stratum lucidum in the CA3 region of the hippocampus which exhibited densely stained mossy fibers and terminals. Mechanical and ischemic lesions induced intense immunolabeling of reactive glial cells around the lesion site. The distinct and anatomically restricted localization of the immunostain in adult and lesioned rat brain suggests that 110 kDa LBP-like molecules might have an important function in forebrain structures and may be involved in the response to CNS injury. C1 FRANCIS SCOTT KEY MED CTR,NIA,GERONTOL RES CTR,NATHAN W SHOCK LABS,CELLULAR & MOLEC BIOL LAB,BALTIMORE,MD 21224. NIDR,DEV BIOL & ANOMALIES LAB,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT,BALTIMORE,MD 21205. FISONS CORP,CNS RES LAB,ROCHESTER,NY 14624. NR 42 TC 24 Z9 24 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD AUG 2 PY 1991 VL 555 IS 2 BP 305 EP 312 DI 10.1016/0006-8993(91)90356-Z PG 8 WC Neurosciences SC Neurosciences & Neurology GA GB120 UT WOS:A1991GB12000016 PM 1834310 ER PT J AU BENITO, M PORRAS, A NEBREDA, AR SANTOS, E AF BENITO, M PORRAS, A NEBREDA, AR SANTOS, E TI DIFFERENTIATION OF 3T3-L1 FIBROBLASTS TO ADIPOCYTES INDUCED BY TRANSFECTION OF RAS ONCOGENES SO SCIENCE LA English DT Article ID GROWTH FACTOR-I; FATTY-ACID SYNTHASE; ADIPOSE CELL LINE; H-RAS; MOLECULAR-CLONING; MESSENGER-RNAS; NEURONAL DIFFERENTIATION; RESPONSIVENESS INVITRO; SIGNAL TRANSDUCTION; POTENTIAL MEDIATOR AB Mammalian 3T3-L1 cells differentiate into adipocytes after continuous exposure to pharmacological doses of insulin or physiological doses of insulin-like growth factor I (IGF-1). Expression of transfected ras oncogenes led to differentiation of these cells into adipocytes in the absence of externally added insulin or IGF-I. Cells transfected with normal ras genes or the tyrosine kinase trk oncogene did not differentiate. Transfection with a dominant inhibitory ras mutant resulted in inhibition of differentiation. Exposure of untransfected 3T3-L1 cells to insulin stimulated formation of the active Ras.GTP complex. These observations indicate that Ras proteins participate in signal transduction pathways initiated by insulin and IGF-I in these cells. C1 NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. RI Porras, Almudena/N-2121-2015 OI Porras, Almudena/0000-0002-6495-3308 NR 45 TC 162 Z9 164 U1 0 U2 4 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD AUG 2 PY 1991 VL 253 IS 5019 BP 565 EP 568 DI 10.1126/science.1857988 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FZ347 UT WOS:A1991FZ34700040 PM 1857988 ER PT J AU SOMBOLOS, K KAPETANOS, G KALOGERAS, KT CHRISTODOULOU, A CHROUSOS, GP SYMEONIDIS, P AF SOMBOLOS, K KAPETANOS, G KALOGERAS, KT CHRISTODOULOU, A CHROUSOS, GP SYMEONIDIS, P TI EFFECT ON ATRIAL-NATRIURETIC-PEPTIDE BY BRACING IN SCOLIOSIS - BOSTON BRACE REMOVAL STUDIED IN 10 CASES SO ACTA ORTHOPAEDICA SCANDINAVICA LA English DT Article ID PLASMA AB The immediate effect of Boston brace removal on plasma levels of atrial natriuretic peptide and arginine vasopressin was studied in 10 patients with idiopathic scoliosis ranging in age from 12 to 16 years. Blood samples were drawn via the antecubital vein in the supine position with the brace on and 10 min after brace removal. ANP and AVP plasma levels were measured by radioimmunoassay. Although no differences were found in the plasma concentrations of arginine vasopressin while on and after removal of the brace, atrial natriuretic peptide levels showed an approximately 80 percent increase 10 min after brace removal, presumably due to central volume redistribution. The changes of atrial natriuretic peptide plasma levels observed may be related to previously reported changes of the glomerular filtration rate and urinary sodium excretion following brace removal. C1 G PAPANIKOLAOU HOSP,DEPT ORTHOPED,SALONIKA,GREECE. ARISTOTELIAN UNIV SALONIKA,RENAL UNIT,SALONIKA,GREECE. NICHHD,BETHESDA,MD 20892. NR 13 TC 0 Z9 1 U1 0 U2 0 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0001-6470 J9 ACTA ORTHOP SCAND JI Acta Orthop. Scand. PD AUG PY 1991 VL 62 IS 4 BP 379 EP 382 PG 4 WC Orthopedics SC Orthopedics GA GD695 UT WOS:A1991GD69500018 PM 1831955 ER PT J AU GARTENHAUS, R MICHAELS, F HALL, L GALLO, RC REITZ, MS AF GARTENHAUS, R MICHAELS, F HALL, L GALLO, RC REITZ, MS TI RELATIVE ACTIVITIES OF HIV-1-IIIB AND HIV-1BAL LTR AND TAT IN PRIMARY MONOCYTES AND LYMPHOCYTES SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; T-CELL; MONONUCLEAR PHAGOCYTES; CHLORAMPHENICOL ACETYLTRANSFERASE; TRANSCRIPTION FACTOR; GENE-EXPRESSION; VIRAL GENOME; HTLV-III; INFECTION; IDENTIFICATION AB The mechanisms determining the ability of some but not other strains of human immunodeficiency virus type 1 (HIV-1) to grow in peripheral blood monocyte-macrophages are presently unclear. The tat gene of HIV-1-III(B) which replicates poorly in human macrophages, and the tal gene of HIV-1-BaL, which replicates to high titers in the same cells in transient expression systems with their respective long terminal repeats (LTR) driving a reporter chloramphenicol acetyl transferase (CAT) gene were compared. The authors hypothesized that the tat gene and LTR of BaL might help account for its efficient growth in primary monocyte-macrophages by virtue of a high activity in these cells relative to that of the III(B) tat and LTR. Primary peripheral blood lymphocytes and monocytes were cotransfected with either the HIV-1BaL or HIV-1-III(B) LTR fused to the CAT gene and their respective tat genes. The III(B) tat and LTR were at least as active in primary lymphocytes as the BaL combination, and both tat-LTR pairs were more active in primary lymphocytes than monocytes. The same relative activities were also observed in primary monocytes after in vitro maturation to macrophages prior to transfection. These data strongly suggest that neither the tat gene nor the LTR of HIV-1-III(B) and HIV-1BaL can account for the great ability of the latter or the inability of the former to grow in monocyte-macrophages. RP GARTENHAUS, R (reprint author), NCI,TUMOR CELL BIOL LAB,BLDG 37,ROOM 6A09,BETHESDA,MD 20892, USA. NR 25 TC 6 Z9 6 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD AUG PY 1991 VL 7 IS 8 BP 681 EP 688 DI 10.1089/aid.1991.7.681 PG 8 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA GC166 UT WOS:A1991GC16600006 PM 1931236 ER PT J AU SAMUEL, KP HODGE, DR CHEN, YMA PAPAS, TS AF SAMUEL, KP HODGE, DR CHEN, YMA PAPAS, TS TI NEF PROTEINS OF THE HUMAN IMMUNODEFICIENCY VIRUSES (HIV-1 AND HIV-2) AND SIMIAN IMMUNODEFICIENCY VIRUS (SIV) ARE STRUCTURALLY SIMILAR TO LEUCINE ZIPPER TRANSCRIPTIONAL ACTIVATION FACTORS SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID LONG TERMINAL REPEAT; DNA-BINDING PROTEINS; IMMEDIATE-EARLY GENE; FOS-JUN INTERACTION; HTLV-III; FUNCTIONAL DISSECTION; NUCLEOTIDE-SEQUENCE; MUTATIONAL ANALYSIS; TRANS-ACTIVATOR; KINASE FAMILY AB Analysis of the predicted amino acid sequences of the human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) and of the related simian immunodeficiency virus (SIV) nef gene products (Nef) reveals the presence of a conserved leucine zipper-like repeat with the characteristic 4,3 arrangement of mainly hydrophobic amino acids in the middle (core) region of the proteins, but lacking the basic (DNA binding) domain characteristic of DNA-binding leucine zipper (bZIP) proteins. Also, at the C-terminus of the Nef proteins is a highly acidic sequence (net charge of -5 to -8) stretched over about 40 amino acids, and contains two predicted alpha-helices separated by a beta-turn linker sequence with sequence homology to known activation domains of acidic transcriptional activation factors. Moreover, within this acidic region of transcriptional activators and the homologous sequence within the second Nef alpha-helix, is a potential transcriptional activation consensus sequence (TACS) bounded by a pair of acidic amino acids (aspartic or glutamic acids) at the N-terminus and a highly invariant phenylalanine (hydrophobic), often followed by an acidic (aspartic) residue, at the C-terminus of the sequence. These findings strongly implicate Nef proteins as belonging to a class of non-DNA-binding leucine zipper acidic transcription factors, and provide a structural basis for new approaches to studying Nef function. C1 NCI,FREDERICK CANC RES & DEV CTR,MOLEC ONCOL LAB,FREDERICK,MD 21701. RP SAMUEL, KP (reprint author), NCI,FREDERICK CANC RES & DEV CTR,DYN CORP,PROGRAM RESOURCES INC,FREDERICK,MD 21701, USA. FU NCI NIH HHS [N0I-CO-74102] NR 60 TC 26 Z9 26 U1 0 U2 2 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD AUG PY 1991 VL 7 IS 8 BP 697 EP 706 DI 10.1089/aid.1991.7.697 PG 10 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA GC166 UT WOS:A1991GC16600008 PM 1931237 ER PT J AU LITTEN, RZ ALLEN, JP AF LITTEN, RZ ALLEN, JP TI PHARMACOTHERAPIES FOR ALCOHOLISM - PROMISING AGENTS AND CLINICAL ISSUES SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE PHARMACOTHERAPY; ALCOHOLISM ID CALCIUM-CHANNEL ANTAGONISTS; CONDITIONED TASTE-AVERSION; WITHDRAWAL SYNDROME; DOUBLE-BLIND; ETHANOL INTOXICATION; SUBSTANCE ABUSE; NITROUS-OXIDE; CONSUMMATORY BEHAVIORS; NORADRENERGIC FUNCTION; PHYSICAL-DEPENDENCE AB The past 10 years have witnessed important advances in research on pharmacotherapy for alcoholism. Promising drugs are discussed under six headings: agents to treat alcohol withdrawal; anticraving agents; agents that make drinking an aversive experience; agents to alleviate concomitant psychiatric problems; agents to treat concurrent drug abuse; and amethystic ("sobering-up") agents. Research on the drug classes is summarized and clinical issues surrounding specific agents and alcoholism pharmacotherapy in general are discussed. Finally, long-range therapeutic implications of recent findings on the actions of alcohol on basic mechanisms of the brain are offered. RP LITTEN, RZ (reprint author), NIAAA,DIV CLIN & PREVENT RES,TREATMENT RES BRANCH,ROOM 14C-20,PARKLAWN BLDG,ROCKVILLE,MD 20857, USA. NR 182 TC 89 Z9 90 U1 7 U2 8 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0145-6008 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD AUG PY 1991 VL 15 IS 4 BP 620 EP 633 DI 10.1111/j.1530-0277.1991.tb00570.x PG 14 WC Substance Abuse SC Substance Abuse GA GB914 UT WOS:A1991GB91400006 PM 1928636 ER PT J AU DILSIZIAN, V PERRONEFILARDI, P CANNON, RO FREEDMAN, NMT BACHARACH, SL BONOW, RO AF DILSIZIAN, V PERRONEFILARDI, P CANNON, RO FREEDMAN, NMT BACHARACH, SL BONOW, RO TI COMPARISON OF EXERCISE RADIONUCLIDE ANGIOGRAPHY WITH THALLIUM SPECT IMAGING FOR DETECTION OF SIGNIFICANT NARROWING OF THE LEFT CIRCUMFLEX CORONARY-ARTERY SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID LEFT-VENTRICULAR FUNCTION; EMISSION COMPUTED-TOMOGRAPHY; QUANTITATIVE-ANALYSIS; TL-201 TOMOGRAPHY; DISEASE; DIAGNOSIS; SCINTIGRAPHY; BYPASS; REST; ANGIOCARDIOGRAPHY AB Although quantitation of exercise thallium tomograms has enhanced the noninvasive diagnosis and localization of coronary artery disease, the detection of stenosis of the left circumflex coronary artery remains suboptimal. Because posterolateral regional wall motion during exercise is well assessed by radionuclide angiography, this study determined whether regional dysfunction of the posterolateral wall during exercise radionuclide angiography is more sensitive in identifying left circumflex disease than thallium perfusion abnormalities assessed by single-photon emission computed tomography (SPECT). One hundred ten consecutive patients with CAD were studied, of whom 70 had a significant stenosis of the left circumflex coronary artery or a major obtuse marginal branch. Both regional function and segmental thallium activity of the posterolateral wall were assessed using visual and quantitative analysis. Left ventricular regional function was assessed objectively by dividing the left ventricular region of interest into 20 sectors; the 8 sectors corresponding to the posterolateral free wall were used to assess function in the left circumflex artery distribution. Similarly, using circumferential profile analysis of short-axis thallium tomograms, left ventricular myocardial activity was subdivided into 64 sectors; the 16 sectors corresponding to the posterolateral region were used to assess thallium perfusion abnormalities in the left circumflex artery territory. Qualitative posterolateral wall motion analysis detected 76% of patients with left circumflex coronary artery stenosis, with a specificity of 83%, compared with only 44% by qualitative thallium tomography (p < 0.001) and a specificity of 92%. Whereas quantitation of thallium activity increased the sensitivity for detecting left circumflex coronary artery stenosis to 80% with a specificity of 55%, it did not achieve statistical significance when compared with qualitative wall motion analysis. Similarly, quantitation of the posterolateral regional function did not improve the sensitivity for detecting left circumflex coronary artery stenosis (74%) when compared with qualitative regional function. A similar analysis applied to the interventricular septum revealed a higher predictive accuracy for detecting left anterior descending coronary artery stenosis by qualitative thallium SPECT, with a sensitivity of 80% and a specificity of 71%, compared with a sensitivity of 68% and a specificity of 57% by qualitative radionuclide angiography. Quantitation of thallium activity in both septal and anterior regions provided no additional insight for detection of left anterior descending artery stenosis. Thus, for noninvasive detection of left circumflex coronary artery disease, the data suggest that qualitative exercise radionuclide angiography is preferable to qualitative thallium SPECT and provides comparable information to quantitative thallium analysis. C1 NIH,DEPT NUCL MED,BETHESDA,MD 20892. RP DILSIZIAN, V (reprint author), NHLBI,CARDIOL BRANCH,BLDG 10,ROOM 7B-15,BETHESDA,MD 20892, USA. NR 25 TC 9 Z9 9 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD AUG 1 PY 1991 VL 68 IS 4 BP 320 EP 328 DI 10.1016/0002-9149(91)90826-7 PG 9 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA FY653 UT WOS:A1991FY65300006 PM 1858674 ER PT J AU KORETZ, DS AF KORETZ, DS TI PREVENTION-CENTERED SCIENCE IN MENTAL-HEALTH SO AMERICAN JOURNAL OF COMMUNITY PSYCHOLOGY LA English DT Article RP KORETZ, DS (reprint author), NIMH,PREVENT RES BRANCH,5600 FISHERS LN,ROCKVILLE,MD 20857, USA. NR 2 TC 22 Z9 22 U1 0 U2 1 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0091-0562 J9 AM J COMMUN PSYCHOL JI Am. J. Community Psychol. PD AUG PY 1991 VL 19 IS 4 BP 453 EP 458 PG 6 WC Public, Environmental & Occupational Health; Psychology, Multidisciplinary; Social Work SC Public, Environmental & Occupational Health; Psychology; Social Work GA GL391 UT WOS:A1991GL39100001 PM 1755430 ER PT J AU HEISS, G SHARRETT, AR BARNES, R CHAMBLESS, LE SZKLO, M ALZOLA, C AF HEISS, G SHARRETT, AR BARNES, R CHAMBLESS, LE SZKLO, M ALZOLA, C TI CAROTID ATHEROSCLEROSIS MEASURED BY B-MODE ULTRASOUND IN POPULATIONS - ASSOCIATIONS WITH CARDIOVASCULAR RISK-FACTORS IN THE ARIC STUDY SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE ARTERIOSCLEROSIS; CARDIOVASCULAR DISEASES; HYPERTENSION; LIPOPROTEINS; SMOKING; ULTRASONICS ID THICKNESS; WALL AB To assess whether carotid atherosclerosis measured by B-mode ultrasound is related to cardiovascular risk factors, 386 cases with carotid artery wall thickening and an equal number of controls free of arterial intima-media thickening were drawn from the cohort of the Atherosclerosis Risk in Communities (ARIC) Study examined in four communities in the United States between 1988 and 1990. Cases and controls were individually matched on sex, race, age group, study center, and date of examination. The mean values of total cholesterol, low density lipoprotein (LDL) cholesterol, total triglyceride, blood pressure, and pack-years of cigarette smoking were higher in cases than controls. Mean high density lipoprotein (HDL) cholesterol was lower in cases than controls. Case-control differences were all statistically significant. Multivariable-adjusted odds ratios point to differences of considerable magnitude in the risk of carotid atherosclerosis between groups defined by clinical and public health-oriented risk factor cut-points. C1 NHLBI,DIV EPIDEMIOL & CLIN APPLICAT,BETHESDA,MD 20892. ARIC ULTRASOUND READING CTR,WINSTON SALEM,NC. UNIV N CAROLINA,SCH PUBL HLTH,DEPT BIOSTAT,CHAPEL HILL,NC 27514. JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT EPIDEMIOL,BALTIMORE,MD 21218. RP HEISS, G (reprint author), UNIV N CAROLINA,SCH PUBL HLTH,DEPT EPIDEMIOL,CB 7400,MCGAVRAN GREENBERG HALL,CHAPEL HILL,NC 27599, USA. FU NHLBI NIH HHS [N01-HC-55015, N01-HC-55018, N01-HC-55016] NR 27 TC 684 Z9 706 U1 1 U2 6 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD AUG 1 PY 1991 VL 134 IS 3 BP 250 EP 256 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GB746 UT WOS:A1991GB74600002 PM 1877584 ER PT J AU FREEDMAN, LS CARROLL, RJ WAX, Y AF FREEDMAN, LS CARROLL, RJ WAX, Y TI ESTIMATING THE RELATION BETWEEN DIETARY-INTAKE OBTAINED FROM A FOOD FREQUENCY QUESTIONNAIRE AND TRUE AVERAGE INTAKE SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE MEASUREMENT ERROR; QUESTIONNAIRES; REGRESSION ANALYSIS ID MEASUREMENT ERROR; DESIGN; VALIDITY; RECALL AB Knowledge of the regression relation between dietary intake reported on a food frequency questionnaire and true average intake is useful in interpreting results from nutritional epidemiologic studies and in planning such studies. Studies which validate a questionnaire against a food record may be used to estimate this regression relation provided the food record is completed by each subject on at least two occasions. Using data collected from women aged 45-69 years during 1985-1986 in the pilot study of the Women's Health Trial, the authors show how variation in diet over time and intraindividual correlation between a questionnaire and food record obtained close together in time affects the estimation of the regression. The authors' method provides estimates of the regression slope and the questionnaire "bias" that are corrected for these effects, together with standard errors. A computer program in the SAS language, for carrying out the analysis, is provided. C1 TEXAS A&M UNIV SYST,DEPT STAT,COLLEGE STN,TX 77843. HEBREW UNIV JERUSALEM,DEPT STAT,IL-91905 JERUSALEM,ISRAEL. RP FREEDMAN, LS (reprint author), NCI,DCPC,BIOMETRY BRANCH,EXECUT PLAZA N,SUITE 344,BETHESDA,MD 20892, USA. FU NIGMS NIH HHS [GM-39015] NR 14 TC 75 Z9 79 U1 1 U2 4 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD AUG 1 PY 1991 VL 134 IS 3 BP 310 EP 320 PG 11 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GB746 UT WOS:A1991GB74600010 PM 1877589 ER PT J AU DEVOR, EJ CLONINGER, CR HOFFMAN, PL TABAKOFF, B AF DEVOR, EJ CLONINGER, CR HOFFMAN, PL TABAKOFF, B TI A GENETIC-STUDY OF PLATELET ADENYLATE-CYCLASE ACTIVITY - EVIDENCE FOR A SINGLE MAJOR LOCUS EFFECT IN FLUORIDE-STIMULATED ACTIVITY SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Article ID COMPLEX SEGREGATION ANALYSIS; ALCOHOLISM; HETEROGENEITY; MODEL AB The activity of membrane-bound platelet adenylate cyclase, when stimulated in vitro by several compounds (including fluoride), is significantly reduced in alcoholics compared with control subjects. We have begun a study of the genetics of this enzyme activity. Complex segregation analysis of basal (unstimulated) platelet adenylate cyclase activity in families reveals a mode of inheritance that cannot be accounted for by a simple mixed model of transmission. By contrast, adenylate cyclase activity stimulated by fluoride ion reveals a single major locus effect with a modest multifactorial background. These results suggest that a single factor in the second-messenger pathway may (a) account for the majority of individual differences in stimulation of adenylate cyclase of fluoride and (b) help explain the reduced activities previously observed in alcoholics. C1 NIAAA,DIV INTRAMURAL CLIN & BIOL RES,BETHESDA,MD. UNIV COLORADO,HLTH SCI CTR,DEPT PHARMACOL,DENVER,CO 80262. RP DEVOR, EJ (reprint author), WASHINGTON UNIV,SCH MED,DEPT PSYCHIAT,4940 AUDUBON,ST LOUIS,MO 63110, USA. RI Cloninger, Claude/F-5357-2012 OI Cloninger, Claude/0000-0003-3096-4807 FU NIAAA NIH HHS [AA-03539]; NIMH NIH HHS [MH-31302] NR 21 TC 26 Z9 26 U1 0 U2 2 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD AUG PY 1991 VL 49 IS 2 BP 372 EP 377 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA GA866 UT WOS:A1991GA86600013 PM 1867196 ER PT J AU KARDAUN, JWPF HAYES, RB POTTERN, LM BROWN, LM HOOVER, RN AF KARDAUN, JWPF HAYES, RB POTTERN, LM BROWN, LM HOOVER, RN TI TESTICULAR CANCER IN YOUNG MEN AND PARENTAL OCCUPATIONAL EXPOSURE SO AMERICAN JOURNAL OF INDUSTRIAL MEDICINE LA English DT Article DE SEMINOMA; GERMINAL TUMORS; PRENATAL EXPOSURE ID UNITED-STATES; RISK-FACTORS AB To investigate whether parental occupation, especially during the 12 month period before birth, could be responsible for elevated rates of testicular cancer in young men, we used data from a case-control study of 223 cases and 212 controls conducted in the Washington, DC area. For all histologic types of testicular cancer combined, no significant associations were found for specific occupations, nor for the broad occupational categories of professional, other white collar, or blue collar workers. However, for cases with seminomas, excess risks were seen for those with parents employed in the following occupations: mothers in health-related occupations, O.R. = 4.6 (1.1-19.1), and fathers working in automobile service stations, O.R. = 4.0 (0.6-24.5), manufacturing industries, O.R. = 2.2 (1.0-4.2), and aircraft production and maintenance, O.R. = 5.3 (0.7-24.1), Although these findings for seminoma are intriguing, they do not explain the increase of testicular cancer in young men. C1 NCI,ENVIRONM EPIDEMIOL BRANCH,EPIDEMIOL & BIOSTAT PROGRAM,EPN 418,BETHESDA,MD 20892. NR 16 TC 22 Z9 22 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0271-3586 J9 AM J IND MED JI Am. J. Ind. Med. PD AUG PY 1991 VL 20 IS 2 BP 219 EP 227 DI 10.1002/ajim.4700200208 PG 9 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FY040 UT WOS:A1991FY04000007 PM 1951369 ER PT J AU BALE, SJ AMOS, CI PARRY, DM BALE, AE AF BALE, SJ AMOS, CI PARRY, DM BALE, AE TI RELATIONSHIP BETWEEN HEAD CIRCUMFERENCE AND HEIGHT IN NORMAL ADULTS AND IN THE NEVOID BASAL-CELL CARCINOMA SYNDROME AND NEUROFIBROMATOSIS TYPE-I SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE OCCIPITO-FRONTAL; MACROCEPHALY; ADULT NORMAL STANDARDS; NF1; NEVOID BASAL CELL CARCINOMA SYNDROME AB Occipitofrontal circumference (OFC) was strongly correlated with height in 72 normal Caucasian men (r = 0.28, P = 0.018) and 78 women (r = 0.53, P < 0.0001). OFC:height ratios were approximately normally distributed in each sex with a mean of 0.326 (standard deviation [sd] = 0.0139) in males and 0.335 (sd = 0.0177) in females. Relative macrocephaly (an OFC greater than the 95th centile for height) was seen in seven of nine probands with the nevoid basal cell carcinoma syndrome (NBCC), eight of 32 non-probands with NBCC, three of four neurofibromatosis type 1 (NF1) probands, and six of nine non-probands with NF1. Thirty-one percent of the non-proband NBCC cases had OFC less than the 50th centile for height, while none of the NF1 cases had this finding. Head size appears to be related to proband status in NBCC, while the evidence suggests that NF1 is a true macrocephaly syndrome. Objective detection of relative abnormalities in head size may aid in syndrome delineation and diagnosis. C1 NCI,DIV CANC ETIOL,CLIN EPIDEMIOL BRANCH,BETHESDA,MD 20892. RP BALE, SJ (reprint author), NCI,DIV CANC ETIOL,ENVIRONM EPIDEMIOL BRANCH,FAMILY STUDIES SECT,BETHESDA,MD 20892, USA. NR 17 TC 45 Z9 45 U1 1 U2 3 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD AUG 1 PY 1991 VL 40 IS 2 BP 206 EP 210 DI 10.1002/ajmg.1320400217 PG 5 WC Genetics & Heredity SC Genetics & Heredity GA FY246 UT WOS:A1991FY24600016 PM 1910262 ER PT J AU BARNES, R MASOOD, S BARKER, E ROSENGARD, AM COGGIN, DL CROWELL, T KING, CR PORTERJORDAN, K WARGOTZ, ES LIOTTA, LA STEEG, PS AF BARNES, R MASOOD, S BARKER, E ROSENGARD, AM COGGIN, DL CROWELL, T KING, CR PORTERJORDAN, K WARGOTZ, ES LIOTTA, LA STEEG, PS TI LOW NM23 PROTEIN EXPRESSION IN INFILTRATING DUCTAL BREAST CARCINOMAS CORRELATES WITH REDUCED PATIENT SURVIVAL SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Note ID DROSOPHILA DEVELOPMENT; TUMOR-METASTASIS AB Protein levels corresponding to nm23 were determined in normal and neoplastic breast tissues by immunoperoxidase staining. Nm23 protein levels were highest in normal breast epithelium, and lower in intraductal carcinomas. Based on nm23 staining, 39 infiltrating ductal carcinomas were separated into two groups: tumors with homogeneously high nm23 protein content, and tumors with low staining in either a homogeneous or heterogeneous pattern. Patients with low nm23 staining tumors, determined by three pathologists independently, had reduced survival times (alpha = 0.034, alpha = 0.012, alpha = 0.052 by the log rank test). Nm23 expression approached significance as an independent predictor of survival in Cox's proportional hazards model. The data provide the first correlation of low nm23 protein expression and reduced breast carcinoma patient survival. C1 MOLEC ONCOL INC,GAITHERSBURG,MD. NCI,PATHOL LAB,BETHESDA,MD 20892. DOCTORS COMMUNITY HOSP,DIV PATHOL,LANHAM,MD. RP BARNES, R (reprint author), UNIV FLORIDA,HLTH SCI CTR,DEPT PATHOL,JACKSONVILLE DIV,JACKSONVILLE,FL 32209, USA. NR 9 TC 193 Z9 197 U1 0 U2 1 PU AMER SOC INVESTIGATIVE PATHOLOGY, INC PI BALTIMORE PA 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD AUG PY 1991 VL 139 IS 2 BP 245 EP 250 PG 6 WC Pathology SC Pathology GA GA982 UT WOS:A1991GA98200001 PM 1714241 ER PT J AU BATES, SE SHIEH, CY TSOKOS, M AF BATES, SE SHIEH, CY TSOKOS, M TI EXPRESSION OF MDR-1/P-GLYCOPROTEIN IN HUMAN NEUROBLASTOMA SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Article ID HUMAN NEURO-BLASTOMA; MULTIDRUG-RESISTANCE GENE; ACID-INDUCED DIFFERENTIATION; CALCIUM-CHANNEL BLOCKERS; OVARY CELL MUTANTS; P-GLYCOPROTEIN; N-MYC; MEMBRANE GLYCOPROTEIN; DRUG-RESISTANCE; MDR1 GENE AB Increased expression of the mdr-1 gene encoding the drug efflux pump P-glycoprotein is a well-established mediator of acquired drug resistance in vitro, and a similar role has been hypothesized in vivo in human malignancy. Because expression of mdr-1 is increased in neuroblastoma cell lines by differentiating agents, the authors hypothesized a similar correlation with differentiation in vivo in neuroblastomas. In 12 tumors from 11 patients, total RNA analysis demonstrated no correlation with differentiation, but a correlation could be detected in the cell-based methods of analysis. The very primitive 'stroma'-poor, poorly differentiated neuroblastomas had low levels of mdr-1/P-glycoprotein. The intermediate grades had higher levels of expression and although heterogeneity of differentiation appeared within these tumors, both primitive and more differentiated cells expressed the gene at comparable levels within the tumor. One very well-differentiated neuroblastoma, a ganglioneuroma, had no detectable expression in the neurofibrillary material, but demonstrated expression in adjacent large ganglionic cells. Thus mdr-1/P-glycoprotein expression increased with increasing differentiation among tumors, and was present in ganglionic cells in the most well-differentiated tumor. The three tumors with the highest levels of expression were obtained from patients who received preoperative chemotherapy. C1 NCI,PATHOL LAB,BETHESDA,MD 20892. RP BATES, SE (reprint author), NCI,MED BRANCH,BLDG 10,RM 12N226,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 38 TC 38 Z9 39 U1 0 U2 1 PU AMER SOC INVESTIGATIVE PATHOLOGY, INC PI BALTIMORE PA 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD AUG PY 1991 VL 139 IS 2 BP 305 EP 315 PG 11 WC Pathology SC Pathology GA GA982 UT WOS:A1991GA98200008 PM 1678252 ER PT J AU REHM, S LIJINSKY, W SINGH, G KATYAL, SL AF REHM, S LIJINSKY, W SINGH, G KATYAL, SL TI MOUSE BRONCHIOLAR CELL CARCINOGENESIS - HISTOLOGIC CHARACTERIZATION AND EXPRESSION OF CLARA CELL ANTIGEN IN LESIONS INDUCED BY N-NITROSOBIS-(2-CHLOROETHYL) UREAS SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Article ID RAT LUNG SURFACTANT; HAN-NMRI MICE; NEOPLASTIC LESIONS; RESPIRATORY-TRACT; FOOD RESTRICTION; TUMORS; CARCINOMA; HISTOGENESIS; LOCALIZATION; CANCER AB Female Swiss mice (Cr:NIH(S)) developed bronchiolar cell hyperplasia, dysplasia, metaplasia, and various morphologic types of bronchiolar cell tumors after topical (skin) application of N-nitroso-methyl-bis-chloroethylurea (NMBCU) or N-nitroso-tris-chloroethylurea (NTCU). These compounds are the first found to induce systemically bronchiolar cell tumors in mice in high incidence. Twice a week, with a 3-day interval, a 25-mu-l drop of 0.04 mol/l (molar) NMBCU or NTCU in acetone was applied to the shaved interscapular integument for a maximum of 35 to 40 weeks. The earliest lung neoplasms were seen in mice that died after 23 weeks of treatment and affected 11 of 19 with NMBCU and 14 of 19 with NTCU treatment. Tumor growth pattern was nodular or the neoplastic tissue was frequently disseminated throughout the parenchyma, starting from multicentric peribronchiolar foci. The most common tumor types were squamous cell carcinomas and adenosquamous carcinomas, followed by adenocarcinomas with or without secretory cells, and a single ciliated-cell tumor. Histochemical and immunohistochemical studies were carried out on paraffin-embedded lungs using the avidin-biotin immunoper-oxidase complex procedure and antisera against keratin, Clara cell antigen, surfactant apoprotein, neuron-specific enolase, bombesin, and chromogranin A. In several mice from both groups, hyperplasias and tumors were composed of cells expressing Clara cell antigen. No tumor cells were found expressing alveolar type II or neuroendocrine cell markers. It appeared that bronchiolar cells, in particular Clara cells, had migrated from terminal bronchioles or invaded bronchiolar walls to extend into the alveolar parenchyma. Squamous cell metaplasia with keratin expression was seen within airways or associated with glandular tumors, especially at the periphery. A unique cell type, with large eosinophilic globules and associated eosinophilic crystals, was seen lining airways or forming hyperplastic and neoplastic lesions. N-nitroso-methyl-bis-chloroethylurea- and NTCU-induced mouse bronchiolar cell alterations could be an interesting new model to study mechanisms of bronchiolar cell differentation and tumor formation. C1 NCI,DIV CANC ETIOL,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21701. ABL BASIC RES PROGRAM,FREDERICK,MD. UNIV PITTSBURGH,DEPT PATHOL,PITTSBURGH,PA 15260. VET ADM MED CTR,SCH MED,PITTSBURGH,PA. FU NCI NIH HHS [N01-CO-74101] NR 44 TC 30 Z9 30 U1 0 U2 2 PU AMER SOC INVESTIGATIVE PATHOLOGY, INC PI BALTIMORE PA 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD AUG PY 1991 VL 139 IS 2 BP 413 EP 422 PG 10 WC Pathology SC Pathology GA GA982 UT WOS:A1991GA98200017 PM 1651059 ER PT J AU OCONNELL, PG LEVINSON, SF AF OCONNELL, PG LEVINSON, SF TI EXPERIENCE WITH REHABILITATION IN THE ACQUIRED-IMMUNODEFICIENCY-SYNDROME SO AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION LA English DT Article DE AIDS, REHABILITATION; DISABILITY; NEUROLOGIC MANIFESTATIONS; PHYSICAL THERAPY; OCCUPATIONAL THERAPY ID AIDS-RELATED COMPLEX; VIRUS-INFECTION; NEUROLOGIC MANIFESTATIONS; CLINICAL-FEATURES; 2',3'-DIDEOXYINOSINE; PROFILE; CARE AB Patients with the acquired immunodeficiency syndrome (AIDS) represent a novel referral population for rehabilitation services. Limited information about the rehabilitation needs of individuals with human immunodeficiency virus infection is available. We reviewed 51 consecutive patients with AIDS referred to a rehabilitation consult service. Common problems encountered included generalized deconditioning (27%) and neurologic dysfunction (45%). Neurologic presentations were diverse and included hemiparesis, diffuse cognitive dysfunction and dementia, myelopathy, myopathy and peripheral neuropathy. Other patients were referred for wound care as well as the management of the local effects of Kaposi's sarcoma, various musculoskeletal syndromes and new onset blindness. Problems identified included impaired mobility (76%), difficulty with self-care (57%), impaired cognition (29%) and uncontrolled pain (37%). Among the rehabilitation interventions utilized were therapeutic exercise (73%), gait aids (45%), bathroom and safety equipment (45%), orthotics (29%), vocational counseling (4%), pain management (29%) and whirlpool treatments (10%). Five patients were too ill or refused treatment. We conclude that AIDS patients referred for rehabilitation have a wide variety of physical deficits, demonstrate a considerable degree of functional impairment and may require multiple rehabilitation interventions. RP OCONNELL, PG (reprint author), NIH,DEPT REHABIL MED,BLDG 10,ROOM 6S235,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 34 TC 14 Z9 16 U1 0 U2 4 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0894-9115 J9 AM J PHYS MED REHAB JI Am. J. Phys. Med. Rehabil. PD AUG PY 1991 VL 70 IS 4 BP 195 EP 200 DI 10.1097/00002060-199108000-00007 PG 6 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA GB568 UT WOS:A1991GB56800007 PM 1878178 ER PT J AU VALDEZ, IH TURNER, RJ AF VALDEZ, IH TURNER, RJ TI EFFECTS OF SECRETAGOGUES ON CYTOSOLIC CA2+ LEVELS IN RAT SUBMANDIBULAR GRANULAR DUCTS AND ACINI SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE FLUID SECRETION; EXOCRINE GLAND; SALIVA; EPIDERMAL GROWTH FACTOR; MICROFLUOROMETRY ID EPIDERMAL GROWTH-FACTOR; EXOCRINE SECRETION; SUBSTANCE-P; GLAND; CELLS; ACETYLCHOLINE; CALCIUM; CA-2+ AB Saliva is thought to be formed by a two-stage process, with the secretion of a "primary fluid" by the acinar cells followed by various ionic modifications in the salivary ducts. Both of these processes are under the control of autonomic stimuli. Although the role of the acini in salivary secretion has been studied in some detail, little is known about properties of ducts, particularly the intralobular ducts that make up the bulk of the ductal tissue. In the present study, microfluorometric methods were employed to examine the responses of intracellular Ca2+ concentration ([Ca2+]i) in individual male rat submandibular acini and intralobular (granular) ducts to various fluid secretory stimuli. We show that granular ducts respond to muscarinic (carbachol) and alpha-adrenergic (epinephrine) stimulation by increasing [Ca2+]i in a manner that is qualitatively similar to acini, but that in contrast to acini, these ducts do not respond to substance P. Because the transduction of a substance P peptidergic signal typically occurs via increased [Ca2+]i, this observation suggests that there are no substance P receptors on granular ducts. Ducts were also found to be somewhat more responsive to both carbachol and epinephrine than acini. Although muscarinic, alpha-adrenergic, and vasoactive intestinal peptide (VIP) stimulation are known to induce the secretion of epidermal growth factor from granular ducts, no significant increase in ductal [Ca2+]i in response to VIP (10(-9) to 10(-6) M) was observed. C1 NIDR,CLIN INVEST & PATIENT CARE BRANCH,BLDG 10,RM 1A06,BETHESDA,MD 20892. NR 19 TC 42 Z9 42 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD AUG PY 1991 VL 261 IS 2 BP G359 EP G363 PN 1 PG 5 WC Physiology SC Physiology GA GC251 UT WOS:A1991GC25100068 PM 1714682 ER PT J AU SHIMONHOPHY, M WADHWANI, KC CHANDRASEKARAN, K LARSON, D SMITH, QR RAPOPORT, SI AF SHIMONHOPHY, M WADHWANI, KC CHANDRASEKARAN, K LARSON, D SMITH, QR RAPOPORT, SI TI REGIONAL BLOOD-BRAIN-BARRIER TRANSPORT OF CATIONIZED BOVINE SERUM-ALBUMIN IN AWAKE RATS SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE CEREBROSPINAL FLUID; PERMEABILITY-SURFACE AREA PRODUCT; TRANSCYTOSIS; CHARGE; CAPILLARIES ID DIFFERENTIATED MICRODOMAINS; CAPILLARY ENDOTHELIUM; CEREBROSPINAL-FLUID; LUMINAL SURFACE; ANIONIC SITES; CHARGE; PERMEABILITY; PROTEIN; KINETICS; PLASMA AB Regional blood-brain barrier permeability-surface area products (PAs) of cationized bovine serum albumin (cBSA) with isoelectric point (pI) almost-equal-to 8 or greater-than-or-equal-to 11 and of native bovine serum albumin (nBSA;pI almost-equal-to 4) were determined in awake male Sprague-Dawley rats after bolus intravenous injection. The albumins were labeled with I-125. Brain uptakes were assessed by autoradiography and by direct assay of radioactivity in brain regions. nBSA uptake into brain was statistically insignificant over 3 h, whereas cBSA uptake was significant even at 6 min. Mean PA values of cBSA with pI almost-equal-to 11 (1.69-2.65 X 10(-5) ml.s-1.g-1) in most brain regions were twofold higher than PAs of cBSA with pI almost-equal-to 8 (0.98-1.37 X 10(-5) ml.s-1.g-1), whereas mean PA for nBSA did not differ significantly from zero. Autoradiographs of brain slices and net distributions in brain compartments at 6 and 30 min after injection suggested that cBSA entered the brain parenchyma via blood vessels and cerebrospinal fluid but that the former was the main route. The results quantitate for the first time regional brain PA values for cationized proteins and suggest specific mechanisms at cerebral blood vessels that distinguish transport of cationized from noncationized macromolecules. RP SHIMONHOPHY, M (reprint author), NIA,NEUROSCI LAB,BLDG 10,ROOM 6C103,BETHESDA,MD 20892, USA. NR 32 TC 20 Z9 21 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD AUG PY 1991 VL 261 IS 2 BP R478 EP R483 PN 2 PG 6 WC Physiology SC Physiology GA GC252 UT WOS:A1991GC25200075 PM 1877704 ER PT J AU SOTSKY, SM GLASS, DR SHEA, MT PILKONIS, PA COLLINS, JF ELKIN, I WATKINS, JT IMBER, SD LEBER, WR MOYER, J OLIVERI, ME AF SOTSKY, SM GLASS, DR SHEA, MT PILKONIS, PA COLLINS, JF ELKIN, I WATKINS, JT IMBER, SD LEBER, WR MOYER, J OLIVERI, ME TI PATIENT PREDICTORS OF RESPONSE TO PSYCHOTHERAPY AND PHARMACOTHERAPY - FINDINGS IN THE NIMH TREATMENT OF DEPRESSION COLLABORATIVE RESEARCH-PROGRAM SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID COGNITIVE THERAPY; FOLLOW-UP; OUTPATIENTS; SUBTYPES; DISORDER; RELAPSE; TRIAL AB Objective: The authors investigated patient characteristics predictive of treatment response in the National Institute of Mental Health (NIMH) Treatment of Depression Collaborative Research Program. Method: Two hundred thirty-nine outpatients with major depressive disorder according to the Research Diagnostic Criteria entered a 16-week multi-center clinical trial and were randomly assigned to interpersonal psychotherapy, cognitive-behavior therapy, imipramine with clinical management, or placebo with clinical management. Pretreatment sociodemographic features, diagnosis, course of illness, function, personality, and symptoms were studied to identify patient predictors of depression severity (measured with the Hamilton Rating Scale for Depression) and complete response (measured with the Hamilton scale and the Beck Depression Inventory). Results: One hundred sixty-two patients completed the entire 16-week trial. Six patient characteristics, in addition to depression severity previously reported, predicted outcome across all treatments: social dysfunction, cognitive dysfunction, expectation of improvement, endogenous depression, double depression, and duration of current episode. Significant patient predictors of differential treatment outcome were identified. 1) Low social dysfunction predicted superior response to interpersonal psychotherapy. 2) Low cognitive dysfunction predicted superior response to cognitive-behavior therapy and to imipramine. 3) High work dysfunction predicted superior response to imipramine. 4) High depression severity and impairment of function predicted superior response to imipramine and to interpersonal psychotherapy. Conclusions: The results demonstrate the relevance of patient characteristics, including social, cognitive, and work function, for prediction of the outcome of major depressive disorder. They provide indirect evidence of treatment specificity by identifying characteristics responsive to different modalities, which may be of value in the selection of patients for alternative treatments. C1 NIMH,MOOD ANXIETY & PERSONAL DISORDERS RES BRANCH,ROCKVILLE,MD 20857. UNIV CHICAGO,SCH SOCIAL SERV ADM,CHICAGO,IL 60637. UNIV OKLAHOMA,HLTH SCI CTR,DEPT PSYCHIAT & BEHAV SCI,OKLAHOMA CITY,OK 73190. WESTERN PSYCHIAT INST & CLIN,PITTSBURGH,PA 15261. VET ADM MED CTR,PERRY POINT,MD 21902. RP SOTSKY, SM (reprint author), GEORGE WASHINGTON UNIV,MED CTR,DEPT PSYCHIAT & BEHAV SCI,2150 PENN AVE NW,WASHINGTON,DC 20037, USA. FU NIMH NIH HHS [MH-33762, MH-33753, MH-33760] NR 57 TC 343 Z9 350 U1 5 U2 43 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD AUG PY 1991 VL 148 IS 8 BP 997 EP 1008 PG 12 WC Psychiatry SC Psychiatry GA FY564 UT WOS:A1991FY56400004 PM 1853989 ER PT J AU ADINOFF, B RISHERFLOWERS, D DEJONG, J RAVITZ, B BONE, GHA NUTT, DJ ROEHRICH, L MARTIN, PR LINNOILA, M AF ADINOFF, B RISHERFLOWERS, D DEJONG, J RAVITZ, B BONE, GHA NUTT, DJ ROEHRICH, L MARTIN, PR LINNOILA, M TI DISTURBANCES OF HYPOTHALAMIC-PITUITARY-ADRENAL AXIS FUNCTIONING DURING ETHANOL WITHDRAWAL IN 6 MEN SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID STRESS AB Objective: Excessive exposure to glucocorticoids can have neurotoxic effects. The behavioral, cognitive, and neurochemical changes observed following the cessation of heavy drinking, therefore, may be associated with disturbances of the hypothalamic-pituitary-adrenal (HPA) axis. To investigate HPA axis disturbances during the ethanol withdrawal syndrome, the authors examined diurnal changes in plasma cortisol in six alcohol-dependent men following the abrupt discontinuation of alcohol intake. Method: Plasma cortisol concentrations were quantified every 30 minutes for 24 hours in the early stage (1 day after cessation) and the middle to late stage (3 days after cessation) of the ethanol withdrawal syndrome as well as after the resolution of acute symptoms (8 days or more after cessation). Results: Plasma cortisol concentrations were almost twice as high during acute withdrawal as they were following recovery. The duration of the cortisol diurnal cycle on the first day of withdrawal was negatively correlated with the severity of withdrawal. Conclusions: There is a marked activation of the HPA axis associated with the ethanol withdrawal syndrome. The authors hypothesize that this activation may account for some of the signs and symptoms of acute and subacute withdrawal. They discuss the potential long-term physiological effects of the episodic increases in cortisol associated with repeated episodes of ethanol withdrawal. The alterations in cortisol rhythmicity during early withdrawal may also have clinical implications. C1 NIAAA,DIV INTRAMURAL CLIN & BIOL RES,CLIN STUDIES LAB,BETHESDA,MD. RI Martin, Peter/A-7738-2008 NR 9 TC 108 Z9 111 U1 1 U2 6 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD AUG PY 1991 VL 148 IS 8 BP 1023 EP 1025 PG 3 WC Psychiatry SC Psychiatry GA FY564 UT WOS:A1991FY56400007 PM 1853950 ER PT J AU PINCUS, HA KENNEDY, C SIMMENS, SJ GOLDMAN, HH SIROVATKA, P SHARFSTEIN, SS AF PINCUS, HA KENNEDY, C SIMMENS, SJ GOLDMAN, HH SIROVATKA, P SHARFSTEIN, SS TI DETERMINING DISABILITY DUE TO MENTAL IMPAIRMENT - APAS EVALUATION OF SOCIAL-SECURITY-ADMINISTRATION GUIDELINES SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article AB Objective: APA evaluated the Social Security Administration's (SSA's) medical standards and guidelines for determining disability due to mental impairment to determine how well the standards and guidelines operationalize the statutory definition of disability in a manner consistent with current psychiatric practice. Method: Seventy-two psychiatrists were trained in one of two procedures: those in the sequential evaluation condition were trained in the process and forms used by the SSA's reviewing medical consultants, and those in the statutory definition condition were trained in the statutory definition of disability and application of clinical judgement according to this standard. Decisions regarding claimants' ability or inability to work were recorded on an instrument designed for the study. Each condition consisted of 12 panels of three members. They reviewed 732 actual claims for disability benefits. The panelists reviewed claims independently, then rendered panel judgements. Each claim was reviewed by one panel in each condition. Results: The proportion of agreement between conditions for panel decisions (0.77) was higher than chance agreement (kappa = 0.46). The high level of agreement on claims judged to have good medical evidence and on which confident decisions were made (proportion of agreement = 0.96, kappa = 0.78) suggests that disagreements largely reflected ambiguities in application of the standards and guidelines to more complex cases or those with inadequate information. Conclusions: The SSA's revised medical standards and guidelines reflect clinical decisions about ability to work based on the statute and, with procedural modifications, should be retained. The SSA should be involved in further systematic studies to develop a field of scientific inquiry into disability and psychiatric disorders. C1 NIMH,DIV APPL & SERV RES,ROCKVILLE,MD 20857. GEORGE WASHINGTON UNIV,MED CTR,DEPT HLTH CARE SCI,WASHINGTON,DC 20037. UNIV MARYLAND,SCH MED,DEPT PSYCHIAT,BALTIMORE,MD 21201. SHEPPARD & ENOCH PRATT HOSP,BALTIMORE,MD 21204. RP PINCUS, HA (reprint author), AMER PSYCHIAT ASSOC,OFF RES,1400 K ST NW,WASHINGTON,DC 20005, USA. NR 9 TC 21 Z9 21 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD AUG PY 1991 VL 148 IS 8 BP 1037 EP 1043 PG 7 WC Psychiatry SC Psychiatry GA FY564 UT WOS:A1991FY56400010 PM 1853953 ER PT J AU ALTEMUS, M HETHERINGTON, MM FLOOD, M LICINIO, J NELSON, ML BERNAT, AS GOLD, PW AF ALTEMUS, M HETHERINGTON, MM FLOOD, M LICINIO, J NELSON, ML BERNAT, AS GOLD, PW TI DECREASE IN RESTING METABOLIC-RATE DURING ABSTINENCE FROM BULIMIC BEHAVIOR SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID THERMOGENESIS; SECRETION; GLUCOSE AB Among eight normal-weight bulimic women studied during 1 week of binge eating and vomiting and 7 weeks of abstinence without weight loss, resting metabolic rate and T3 and T4 levels fell significantly during abstinence. These data suggest that a physiological consequence of binge eating and vomiting is an increase in metabolic rate, in part due to increased thyroid hormone activity. RP ALTEMUS, M (reprint author), NIMH,INTRAMURAL RES PROGRAM,CLIN NEUROENDOCRINOL BRANCH,BLDG 10,BETHESDA,MD 20892, USA. RI Hetherington, Marion/A-4397-2013; Licinio, Julio/L-4244-2013 OI Licinio, Julio/0000-0001-6905-5884 NR 10 TC 16 Z9 16 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD AUG PY 1991 VL 148 IS 8 BP 1071 EP 1072 PG 2 WC Psychiatry SC Psychiatry GA FY564 UT WOS:A1991FY56400017 PM 1853959 ER PT J AU LINN, S FULWOOD, R CARROLL, M BROOK, JG JOHNSON, C KALSBEEK, WD RIFKIND, BM AF LINN, S FULWOOD, R CARROLL, M BROOK, JG JOHNSON, C KALSBEEK, WD RIFKIND, BM TI SERUM TOTAL CHOLESTEROL - HDL CHOLESTEROL RATIOS IN US WHITE AND BLACK ADULTS BY SELECTED DEMOGRAPHIC AND SOCIOECONOMIC VARIABLES (HANES-II) SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID DENSITY LIPOPROTEIN CHOLESTEROL; CORONARY HEART-DISEASE; PROGRAM AB Background: Framingham Study findings suggest that total cholesterol (TC):High density lipoprotein cholesterol (HDL-C) ratio is a useful summary of the joint contribution of TC and HDL-C to coronary heart disease (CHD) risk. Information on the distribution of TC:HDL-C in the US population is limited to selected populations and the relationship of the ratio distribution and its correlates has received little attention. Method: TC/HDL-C ratios were examined in a representative sample of the United States adult population ages 20 to 74 years, between February 1976 and February 1980 during NHANES II, using stratification and multivariate regression analyses. Results: Age-adjusted mean ratios were higher in men compared with women and were higher in Whites compared with Blacks. White men had the highest TC/HDL-C mean ratios. These relationships remained after stratification by age, education, body mass index, alcohol use, cigarette smoking, and physical activity. Using multivariate analyses, the ratios were positively related to BMI, age, and smoking; and negatively related to female sex, alcohol use, being Black, and physical activity. Conclusions: Using a ratio reference point of greater than or equal to 4.5 from the Framingham study, at least an estimated 44 million persons ages 25 to 74 years in the US were found to be at higher risk of developing coronary heart disease. C1 NHLBI,DIV HEART & VASC DIS,LIPID METAB ATHEROGENESIS BRANCH,BETHESDA,MD 20892. NATL CTR HLTH STAT,NUTR STAT BRANCH,HYATTSVILLE,MD 20782. UNIV N CAROLINA,CTR COLLABORAT STUDIES COORDINATING,DEPT BIOSTAT,CHAPEL HILL,NC 27514. NR 17 TC 34 Z9 34 U1 0 U2 0 PU AMER PUBLIC HEALTH ASSN INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD AUG PY 1991 VL 81 IS 8 BP 1038 EP 1043 DI 10.2105/AJPH.81.8.1038 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GD219 UT WOS:A1991GD21900016 PM 1853996 ER PT J AU HAVERKOS, HW STEEL, E AF HAVERKOS, HW STEEL, E TI CRACK COCAINE, FELLATIO, AND THE TRANSMISSION OF HIV SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Letter ID UNITED-STATES C1 NIDA,LEXINGTON,KY 40583. NR 6 TC 7 Z9 7 U1 0 U2 0 PU AMER PUBLIC HEALTH ASSN INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD AUG PY 1991 VL 81 IS 8 BP 1078 EP 1079 DI 10.2105/AJPH.81.8.1078 PG 2 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GD219 UT WOS:A1991GD21900032 PM 1854010 ER PT J AU KHALIL, N OCONNOR, RN UNRUH, HW WARREN, PW FLANDERS, KC KEMP, A BEREZNAY, OH GREENBERG, AH AF KHALIL, N OCONNOR, RN UNRUH, HW WARREN, PW FLANDERS, KC KEMP, A BEREZNAY, OH GREENBERG, AH TI INCREASED PRODUCTION AND IMMUNOHISTOCHEMICAL LOCALIZATION OF TRANSFORMING GROWTH-FACTOR-BETA IN IDIOPATHIC PULMONARY FIBROSIS SO AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY LA English DT Article ID COLLAGEN-SYNTHESIS; RESPIRATORY-TRACT; EPITHELIAL-CELLS; INFLAMMATION; RELEASE; MACROPHAGES; EXPRESSION; BLEOMYCIN; RATS; DIFFERENTIATION AB Transforming growth factor-beta (TGF-beta) can regulate cell growth and differentiation as well as production of extracellular matrix proteins. Elevated production of TGF-beta has been associated with human and rodent chronic inflammatory and fibrotic diseases. Using immunohistochemical staining, we have examined lung sections of patients with advanced idiopathic pulmonary fibrosis (IPF), a disease characterized by chronic inflammation and fibrosis and demonstrated a marked and consistent increase in TGF-beta production in epithelial cells and macrophages when compared to patients with nonspecific inflammation and those with no inflammation or fibrosis. In patients with advanced IPF, intracellular staining with anti-LC (1-30) TGF-beta antibody was seen prominently in bronchiolar epithelial cells. In addition, epithelial cells of honeycomb cysts and hyperplastic type II pneumocytes stained intensely. Anti-CC (1-30) TGF-beta antibody, which reacts with extracellular TGF-beta, was localized in the lamina propria of bronchioles and in subepithelial regions of honeycomb cysts in areas of dense fibroconnective tissue deposition. The close association of subepithelial TGF-beta to the intracellular form in advanced IPF suggests that TGF-beta was produced and secreted primarily by epithelial cells. Because of the well-known effects of TGF-beta on extracellular matrix formation and on epithelial cell differentiation, the increased production of TGF-beta in advanced IPF may be pathogenic to the pulmonary fibrotic and regenerative responses seen in this disease. C1 UNIV MANITOBA,DEPT PATHOL,WINNIPEG R3T 2N2,MANITOBA,CANADA. UNIV MANITOBA,DEPT SURG,WINNIPEG R3T 2N2,MANITOBA,CANADA. UNIV MANITOBA,DEPT PEDIAT,WINNIPEG R3T 2N2,MANITOBA,CANADA. NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. RP KHALIL, N (reprint author), UNIV MANITOBA,DEPT MED,MANITOBA INST CELL BIOL,100 OLIVIA ST,WINNIPEG R3E 0V9,MANITOBA,CANADA. NR 35 TC 387 Z9 393 U1 0 U2 7 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 1044-1549 J9 AM J RESP CELL MOL JI Am. J. Respir. Cell Mol. Biol. PD AUG PY 1991 VL 5 IS 2 BP 155 EP 162 PG 8 WC Biochemistry & Molecular Biology; Cell Biology; Respiratory System SC Biochemistry & Molecular Biology; Cell Biology; Respiratory System GA GA232 UT WOS:A1991GA23200009 PM 1892646 ER PT J AU KORN, EL SIMON, R AF KORN, EL SIMON, R TI EXPLAINED RESIDUAL VARIATION, EXPLAINED RISK, AND GOODNESS OF FIT SO AMERICAN STATISTICIAN LA English DT Article DE BINARY DATA; COEFFICIENT OF DETERMINATION; LOSS FUNCTION; R2; SURVIVAL ANALYSIS ID CATEGORICAL DATA; SURVIVAL-DATA; REGRESSION; R2; RESPONSES; VARIANCE AB A loss function approach is used to define the concepts of explained residual variation and explained risk for general regression models. Explained risk measures the ability of the covariates in a correctly specified model to distinguish differing outcomes. Explained residual variation, which is R2 for a linear model, estimates the explained risk with a penalty for poorly fitting models. Application of the general definitions to linear regression, logistic regression, and survival analysis is given. The importance of distinguishing the concepts of explained residual variation, explained risk, and goodness of fit is discussed. RP KORN, EL (reprint author), NCI,BIOMETR RES BRANCH,BETHESDA,MD 20892, USA. NR 24 TC 44 Z9 44 U1 0 U2 8 PU AMER STATIST ASSN PI ALEXANDRIA PA 1429 DUKE ST, ALEXANDRIA, VA 22314 SN 0003-1305 J9 AM STAT JI Am. Stat. PD AUG PY 1991 VL 45 IS 3 BP 201 EP 206 DI 10.2307/2684290 PG 6 WC Statistics & Probability SC Mathematics GA FY393 UT WOS:A1991FY39300007 ER PT J AU SAKAIRI, M YERGEY, AL AF SAKAIRI, M YERGEY, AL TI ATMOSPHERIC-PRESSURE SPRAY AND ELECTROSPRAY MASS-SPECTRA OF GLYCINE AND ITS OLIGOPEPTIDES SO ANALYTICAL SCIENCES LA English DT Article DE ATMOSPHERIC PRESSURE SPRAY MASS SPECTROMETRY; ELECTROSPRAY MASS SPECTROMETRY; GLYCINE; GLYCINE OLIGOPEPTIDE ID SPECTROMETRY; INTERFACE; EVAPORATION; IONIZATION AB Mass spectra of glycine, glycyl-glycine and glycyl-glycyl-glycine obtained by atmospheric pressure spray mass spectrometry (APS-MS) are compared with those obtained by electrospray mass spectrometry (ESP-MS). Intense cationized molecules of (M + Na)+ and (M + K)+ are observed in addition to protonated molecules of (M + H)+ in the atmospheric pressure spray mass spectra, while only protonated molecules are observed in the electrospray mass spectra. This result clearly indicates that the two methods differ greatly as regards ionization process, although sample solutions are sprayed to produce ions under atmospheric pressure in both methods. C1 NICHHD,BETHESDA,MD 20892. RP SAKAIRI, M (reprint author), HITACHI LTD,CENT RES LAB,KOKUBUNJI,TOKYO 185,JAPAN. NR 11 TC 2 Z9 2 U1 1 U2 2 PU JAPAN SOC ANALYTICAL CHEM PI TOKYO PA 26-2 NISHIGOTANDA 1 CHOME SHINAGAWA-KU, TOKYO 141, JAPAN SN 0910-6340 J9 ANAL SCI JI Anal. Sci. PD AUG PY 1991 VL 7 IS 4 BP 589 EP 592 DI 10.2116/analsci.7.589 PG 4 WC Chemistry, Analytical SC Chemistry GA GC015 UT WOS:A1991GC01500012 ER PT J AU MORRISON, JE MATTHEWS, D WASHINGTON, R FENNESSEY, PV HARRISON, LM AF MORRISON, JE MATTHEWS, D WASHINGTON, R FENNESSEY, PV HARRISON, LM TI PHENOL MOTOR POINT BLOCKS IN CHILDREN - PLASMA-CONCENTRATIONS AND CARDIAC DYSRHYTHMIAS SO ANESTHESIOLOGY LA English DT Article DE ANESTHESIA, PEDIATRIC; ANESTHESIA, REGIONAL, MOTOR POINT BLOCK; CARDIAC, DYSRHYTHMIAS; PHENOL, TOXICITY C1 UNIV COLORADO,HLTH SCI CTR,DEPT ANESTHESIOL,DENVER,CO 80262. UNIV COLORADO,HLTH SCI CTR,NIH,MASS SPECTROMETRY RES RESOURCE,DENVER,CO 80262. CHILDRENS HOSP,DEPT REHABIL MED,DENVER,CO 80218. CHILDRENS HOSP,DEPT CARDIOL,DENVER,CO 80218. UNIV COLORADO,HLTH SCI CTR,DEPT PEDIAT,DENVER,CO 80262. UNIV COLORADO,HLTH SCI CTR,DEPT PHARMACOL,DENVER,CO 80262. UNIV COLORADO,HLTH SCI CTR,DEPT REHABIL MED,DENVER,CO 80262. RP MORRISON, JE (reprint author), CHILDRENS HOSP,DEPT ANESTHESIOL,1056 E 19TH AVE,DENVER,CO 80218, USA. FU NCRR NIH HHS [RR01152] NR 9 TC 11 Z9 11 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0003-3022 J9 ANESTHESIOLOGY JI Anesthesiology PD AUG PY 1991 VL 75 IS 2 BP 359 EP 362 DI 10.1097/00000542-199108000-00027 PG 4 WC Anesthesiology SC Anesthesiology GA FZ700 UT WOS:A1991FZ70000027 PM 1859024 ER PT J AU FLEISHER, TA AF FLEISHER, TA TI LABORATORY MEASUREMENTS - IMMUNOGLOBULINS, MEDIATORS, CELL-SURFACE MARKERS, CELL-PROLIFERATION AND PHAGOCYTOSIS SO ANNALS OF ALLERGY LA English DT Article ID NEUTROPHILS RP FLEISHER, TA (reprint author), NIH,CTR CLIN,IMMUNOL SERV,BETHESDA,MD 20892, USA. NR 19 TC 0 Z9 0 U1 0 U2 0 PU AMER COLL ALLERGY ASTHMA IMMUNOLOGY PI ARLINGTON HTS PA 85 WEST ALGONQUIN RD SUITE 550, ARLINGTON HTS, IL 60005 SN 0003-4738 J9 ANN ALLERGY JI Ann. Allergy PD AUG PY 1991 VL 67 IS 2 BP 186 EP 189 PN 2 PG 4 WC Allergy SC Allergy GA GB912 UT WOS:A1991GB91200005 ER PT J AU SANDLER, DP BURR, FR WEINBERG, CR AF SANDLER, DP BURR, FR WEINBERG, CR TI NONSTEROIDAL ANTIINFLAMMATORY DRUGS AND THE RISK FOR CHRONIC RENAL-DISEASE SO ANNALS OF INTERNAL MEDICINE LA English DT Article ID PLASMA-RENIN ACTIVITY; PROSTAGLANDIN SYNTHESIS; ANTIINFLAMMATORY DRUGS; ANALGESIC NEPHROPATHY; FAILURE; INDOMETHACIN; IBUPROFEN; ASPIRIN; INFORMATION; MECHANISM AB Objective: To evaluate the risk for chronic renal disease associated with regular use of nonaspirin nonsteroidal anti-inflammatory drugs (NSAIDs). Design: Multicenter case-control study. Patients: Patients were 554 North Carolina residents (age range, 30 to 79 years) hospitalized between 1980 and 1982 with a discharge diagnosis indicating newly diagnosed chronic renal dysfunction and a serum creatinine level consistently at or above 130-mu-mol/L (1.5 mg/dL). Controls were 516 persons chosen randomly by telephone screening (if younger than 65 years of age) and from listings of Medicare recipients (if 65 years of age or older), frequency-matched to patients by age, race, sex, and proximity to study hospitals. Measurements: Data on use of prescription NSAIDs and other analgesics before 1980, other risk factors, and potential confounders were obtained by telephone interviews. Patients were classified by frequency and duration of use; daily users were those who took an NSAID for at least 360 consecutive days. Main Results: A twofold risk for chronic renal disease was associated with previous daily use of NSAIDs (adjusted odds ratio, 2.1; 95% Cl, 1.1 to 4.1). Increased risk was predominantly limited to men older than 65 years, for whom the odds ratio for daily use was 10.0 (Cl, 1.2 to 82.7) after adjusting for use of other analgesics. In other age-sex groups, the risk associated with NSAID use tended to be increased among those with heart disease or other factors that might indicate compromised renal circulation. These findings did not result from confounding by known renal disease risk factors and were not readily explained by potential biases. Conclusions: Regular use of NSAIDs may increase the risk for chronic kidney disease in some high-risk groups. With the recent over-the-counter availability and increasing popularity of NSAIDs, the possibility of an increased risk for chronic renal disease associated with their use may warrant further scrutiny. RP SANDLER, DP (reprint author), NIEHS,EPIDEMIOL BRANCH,MD A305,POB 12233,RES TRIANGLE PK,NC 27709, USA. OI Sandler, Dale/0000-0002-6776-0018 NR 43 TC 137 Z9 140 U1 0 U2 6 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD AUG 1 PY 1991 VL 115 IS 3 BP 165 EP 172 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA FY152 UT WOS:A1991FY15200001 PM 2058870 ER PT J AU YARCHOAN, R VENZON, DJ PLUDA, JM LIETZAU, J WYVILL, KM TSIATIS, AA STEINBERG, SM BRODER, S AF YARCHOAN, R VENZON, DJ PLUDA, JM LIETZAU, J WYVILL, KM TSIATIS, AA STEINBERG, SM BRODER, S TI CD4 COUNT AND THE RISK FOR DEATH IN PATIENTS INFECTED WITH HIV RECEIVING ANTIRETROVIRAL THERAPY SO ANNALS OF INTERNAL MEDICINE LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; PNEUMOCYSTIS-CARINII PNEUMONIA; AIDS-RELATED COMPLEX; KAPOSIS SARCOMA; HOMOSEXUAL MEN; MONONUCLEAR PHAGOCYTES; NATURAL-HISTORY; ZIDOVUDINE AZT; HTLV-III; PREDICTORS AB Objective: To investigate the relation between CD4 count and the immediate hazard of dying in patients receiving zidovudine (azidothymidine [AZT])-based antiretroviral therapy. Setting: A research hospital that recruits patients from the entire United States. Design: Retrospective analysis of a cohort of patients with the acquired immunodeficiency syndrome (AIDS) or AIDS-related complex participating in long-term zidovudine-based antiretroviral protocols. Patients: Fifty-five patients with human immunodeficiency virus (HIV) infection and either AIDS or severe AIDS-related complex who were followed for as many as 4 years while they received antiretroviral therapy. Measurements: CD4 counts were measured. Main Results: Ten patients are known to be alive and 1 was lost to follow-up. Of the 44 patients who are known to have died, the CD4 range was known within 6 months of death in 41. All but 1 of these 41 assessable deaths occurred in patients whose CD4 counts were known to have fallen below 50 CD4 cells/mm3 (p < 10(-10)). The hazard of dying in the cohort ranged from 0 deaths/patient-month (95% Cl, 0 to 0.008 deaths/patient-month) in patients with 200 or more CD4 cells/mm3 to 0.07 deaths/patient-month (Cl, 0.050 to 0.094 deaths/patient-month) in patients with fewer than 50 CD4 cells/mm3. For the patients who died and whose cases were assessable, the mean of the last three CD4 counts obtained before death was 7.7 CD4 cells/mm3 (Cl, 0.9 to 63.3 cells/mm3). The median survival of patients once their CD4 counts fell below 50 CD4 cells/mm3 was 12.1 months (Cl, 7.2 to 19.4 months). Conclusions: In a carefully followed cohort treated with zidovudine-based antiretroviral therapy, nearly all deaths occurred in patients with fewer than 50 CD4 cells/mm3. These findings may have implications in the monitoring of patients with AIDS and in the use of CD4 count as a clinical trials end point for the antiretroviral therapy of HIV infection. C1 HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. RP YARCHOAN, R (reprint author), NCI,BLDG 10,ROOM 13N248,BETHESDA,MD 20892, USA. RI Venzon, David/B-3078-2008 FU NIAID NIH HHS [AI-24643] NR 37 TC 166 Z9 167 U1 1 U2 1 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD AUG 1 PY 1991 VL 115 IS 3 BP 184 EP 189 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA FY152 UT WOS:A1991FY15200004 PM 1676252 ER PT J AU BARTH, RJ VENZON, DJ BAKER, AR AF BARTH, RJ VENZON, DJ BAKER, AR TI THE PROGNOSIS OF MELANOMA PATIENTS WITH METASTASES TO 2 OR MORE LYMPH-NODE AREAS SO ANNALS OF SURGERY LA English DT Article ID MALIGNANT-MELANOMA; MULTIFACTORIAL ANALYSIS; STAGE-II; DISSECTION; EXTREMITY AB The prognosis of melanoma patients who present with metastatic involvement of two or more noncontiguous lymph node regions before the detection of extranodal metastases has not been previously reported. We identified 21 patients with metastatic melanoma in at least two nodal basins in a review of 175 patients with melanoma undergoing lymphadenectomy at the National Cancer institute. The median survival time of these patients was 46 months, with 55%, 27%, and 17% of the patients alive 2, 5, and 10 years, respectively, after the second lymphadenectomy. Because the prognosis of melanoma patients with metastases to two or more regional nodal areas appears equivalent to that of patients with metastatic involvement of only one regional node site, lymphadenectomy of the involved groups should be performed with therapeutically curative intent. C1 NCI,BIOSTAT & DATA MANAGEMENT SECT,BETHESDA,MD 20892. RP BARTH, RJ (reprint author), NCI,SURG BRANCH,ROOM 2B46,BLDG 10,BETHESDA,MD 20892, USA. RI Venzon, David/B-3078-2008 NR 23 TC 11 Z9 11 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0003-4932 J9 ANN SURG JI Ann. Surg. PD AUG PY 1991 VL 214 IS 2 BP 125 EP 130 DI 10.1097/00000658-199108000-00006 PG 6 WC Surgery SC Surgery GA GB580 UT WOS:A1991GB58000006 PM 1867519 ER PT J AU SALOMON, GD KASID, A CROMACK, DT DIRECTOR, E TALBOT, TL SANK, A NORTON, JA AF SALOMON, GD KASID, A CROMACK, DT DIRECTOR, E TALBOT, TL SANK, A NORTON, JA TI THE LOCAL-EFFECTS OF CACHECTIN TUMOR-NECROSIS-FACTOR ON WOUND-HEALING SO ANNALS OF SURGERY LA English DT Article ID FACTOR-ALPHA; ENDOTHELIAL-CELLS; GROWTH-FACTOR; INTERLEUKIN-1; PROLIFERATION; COLLAGENASE; FIBROBLASTS; BACTEREMIA; EXPRESSION; INDUCTION AB Previous experimental studies have suggested that tumor necrosis factor (TNF) may have either a beneficial or a detrimental role in wound healing. Control and doxorubicin-treated (6 mg/kg, intravenously) rats underwent paired dorsal 5-cm linear wounds and had either vehicle or recombinant (r)TNF (0.5, 5, or 50-mu-g) applied locally to the wound. Paired wounds were harvested at 7 and 14 days after wounding and analyzed for wound-bursting strength (WBS) and activity of the gene for type 1 collagen and TNF. Doxorubicin treatment decreased WBS at 14 days but not at 7 days after wounding. Local application of 50-mu-g of rTNF decreased WBS in saline-treated rats and concentrations of 5 and 50-mu-g decreased WBS in doxorubicin-treated rats when measured 7 days after wounding. These effects dissipated when WBS was measured 14 days after wounding. Doxorubicin decreased wound collagen gene expression and local TNF treatment decreased wound collagen gene expression in saline-treated rats and further decreased it in doxorubicin-treated rats. The decrement in collagen gene expression induced by rTNF increased as the local dose of rTNF increased. The gene for TNF was not detectable in wounds from normal or doxorubicin-treated rats at 3, 7, 10, or 14 days after wounding. These data suggest that the gene for TNF is not expressed in wounds and that the local application of TNF is detrimental to wound healing as it decreases WBS and activity of the gene for collagen. C1 NCI,SURG BRANCH,SURG METAB SECT,BLDG 10,ROOM 2B07,BETHESDA,MD 20892. NR 27 TC 68 Z9 71 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0003-4932 J9 ANN SURG JI Ann. Surg. PD AUG PY 1991 VL 214 IS 2 BP 175 EP 180 DI 10.1097/00000658-199108000-00012 PG 6 WC Surgery SC Surgery GA GB580 UT WOS:A1991GB58000012 PM 1714269 ER PT J AU POGREBNIAK, HW ROTH, JA STEINBERG, SM ROSENBERG, SA PASS, HI AF POGREBNIAK, HW ROTH, JA STEINBERG, SM ROSENBERG, SA PASS, HI TI REOPERATIVE PULMONARY RESECTION IN PATIENTS WITH METASTATIC SOFT-TISSUE SARCOMA SO ANNALS OF THORACIC SURGERY LA English DT Article; Proceedings Paper CT 37TH ANNUAL MEETING OF THE SOUTHERN THORACIC SURGICAL ASSOC CY NOV 08-10, 1990 CL DORADO, PR SP SO THORAC SURG ASSOC ID ADULTS AB Resection of pulmonary metastases from soft tissue sarcoma has been shown to be associated with a 3-year survival of 25% to 30%. The role of multiple resections for recurrent pulmonary metastases, however, has not been clearly defined. Since 1976, 43 patients have had two or more thoracic explorations for the purpose of resecting pulmonary metastases from adult soft tissue sarcoma at our institution. In 89 reexplorations, through either median sternotomy or lateral thoracotomy, the operative mortality was 0%, and 31 of the 43 patients (72%) could be rendered free of disease at the second thoracotomy. Median survival from the second thoracotomy for the patients with resectable disease was 25 months, whereas median survival of patients who had unresectable disease was 10 months. A disease-free interval between the first and second thoracotomies of greater than 18 months was associated with prolonged survival from the second thoracotomy. Owing to lack of other therapies with proven salvage efficacy and in the absence of randomized trials, repeated thoracotomies to render patients free of disease from pulmonary soft-tissue sarcoma metastases appear justified considering the potential survival benefit and low attendant risk. C1 NCI,SURG BRANCH,THORAC ONCOL SECT,BLDG 10,ROOM 2B07,BETHESDA,MD 20892. NCI,BIOSTAT & DATA MANAGEMENT SECT,BETHESDA,MD 20892. NR 11 TC 42 Z9 43 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0003-4975 J9 ANN THORAC SURG JI Ann. Thorac. Surg. PD AUG PY 1991 VL 52 IS 2 BP 197 EP 203 PG 7 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA GB137 UT WOS:A1991GB13700006 PM 1863139 ER PT J AU MORALES, TI JOYCE, ME SOBEL, ME DANIELPOUR, D ROBERTS, AB AF MORALES, TI JOYCE, ME SOBEL, ME DANIELPOUR, D ROBERTS, AB TI TRANSFORMING GROWTH-FACTOR-BETA IN CALF ARTICULAR-CARTILAGE ORGAN-CULTURES - SYNTHESIS AND DISTRIBUTION SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article ID MOLECULAR-WEIGHT COMPLEX; PRECURSOR SEQUENCES; FACTOR-BETA-1 GENE; MOUSE EPIDERMIS; HUMAN-PLATELETS; MESSENGER-RNA; 2 FORMS; PROTEOGLYCANS; CELLS; EXPRESSION C1 NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. NCI,PATHOL LAB,BETHESDA,MD 20892. NIAMSD,ORTHOPED LABS,BETHESDA,MD 20892. RP MORALES, TI (reprint author), NIDR,BONE RES BRANCH,BLDG 30,ROOM 106,BETHESDA,MD 20892, USA. NR 38 TC 103 Z9 105 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD AUG 1 PY 1991 VL 288 IS 2 BP 397 EP 405 DI 10.1016/0003-9861(91)90212-2 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FV096 UT WOS:A1991FV09600013 PM 1716871 ER PT J AU KRAGEL, PJ DEVANEY, KO TRAVIS, WD AF KRAGEL, PJ DEVANEY, KO TRAVIS, WD TI MUCINOUS CYSTADENOMA OF THE LUNG - REPLY SO ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE LA English DT Letter C1 ARMED FORCES INST PATHOL,WASHINGTON,DC 20306. NCI,BETHESDA,MD 20892. RP KRAGEL, PJ (reprint author), UNIV MISSOURI,TRUMAN MED CTR,KANSAS CITY,MO 64108, USA. NR 5 TC 4 Z9 4 U1 0 U2 0 PU COLLEGE AMER PATHOLOGISTS PI NORTHFIELD PA C/O KIMBERLY GACKI, 325 WAUKEGAN RD, NORTHFIELD, IL 60093-2750 SN 0003-9985 J9 ARCH PATHOL LAB MED JI Arch. Pathol. Lab. Med. PD AUG PY 1991 VL 115 IS 8 BP 740 EP 741 PG 2 WC Medical Laboratory Technology; Medicine, Research & Experimental; Pathology SC Medical Laboratory Technology; Research & Experimental Medicine; Pathology GA FZ475 UT WOS:A1991FZ47500002 ER PT J AU LEVINSON, SF OCONNELL, PG AF LEVINSON, SF OCONNELL, PG TI REHABILITATION DIMENSIONS OF AIDS - A REVIEW SO ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION LA English DT Review DE AIDS; DISABILITY; NEUROLOGIC MANIFESTATIONS; OCCUPATIONAL THERAPY; PHYSICAL THERAPY; REHABILITATION ID HUMAN IMMUNODEFICIENCY VIRUS; ACQUIRED-IMMUNODEFICIENCY; HIV-INFECTION; NEUROLOGIC MANIFESTATIONS; CLINICAL-FEATURES; DEMENTIA COMPLEX; CYTOMEGALO-VIRUS; PERIPHERAL-NERVE; TYPE-1 INFECTION; SPINAL-CORD AB With the spread of human immunodeficiency virus (HIV) infection and of the acquired immune deficiency syndrome (AIDS), many rehabilitation professionals are faced with new challenges. This report covers two basic problems that are becoming common in rehabilitation practice: the management of the patient who suffers from disability resulting from HIV infection or AIDS, and the management of the traditional rehabilitation patient who may coincidentally be infected with HIV. Common manifestations of HIV infection and associated secondary infections and neoplasms are discussed, as well as are the complications of current medical treatments. This report also explores specific neurologic and musculoskeletal disorders and the fatigue associated with AIDS. In addition, potential approaches to rehabilitation management are evaluated, and consideration is given to the management of the asymptomatic HIV carrier. The differences between HIV infection in adults and children are explored, and the social implications of HIV rehabilitation are discussed. After consideration of the basic pathophysiology of infection and the modes of transmission, the significance and ethics of serologic testing are examined, and the concept of universal precautions is described. The paper concludes with a discussion of the use of safe sex practices by the disabled population. RP LEVINSON, SF (reprint author), NIH,DEPT REHABIL MED,BLDG 10,ROOM 6S235,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 94 TC 15 Z9 17 U1 0 U2 4 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0003-9993 J9 ARCH PHYS MED REHAB JI Arch. Phys. Med. Rehabil. PD AUG PY 1991 VL 72 IS 9 BP 690 EP 696 PG 7 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA FZ466 UT WOS:A1991FZ46600015 PM 1650171 ER PT J AU STEINBERG, AD STEINBERG, SC AF STEINBERG, AD STEINBERG, SC TI LONG-TERM PRESERVATION OF RENAL-FUNCTION IN PATIENTS WITH LUPUS NEPHRITIS RECEIVING TREATMENT THAT INCLUDES CYCLOPHOSPHAMIDE VERSUS THOSE TREATED WITH PREDNISONE ONLY SO ARTHRITIS AND RHEUMATISM LA English DT Article ID CONTROLLED TRIAL; IMMUNOSUPPRESSIVE DRUGS; FOLLOW-UP; AZATHIOPRINE; THERAPY; GLOMERULONEPHRITIS; ERYTHEMATOSUS AB The purpose of this study was to assess long-term preservation of renal function in 111 patients with systemic lupus erythematosus and active glomerulonephritis who participated in a randomized treatment trial. Four different drug treatment programs, each of which allowed the use of low-dose oral prednisone in addition to the study drug(s), were compared with a regimen consisting solely of high-dose oral prednisone. Patients randomized to receive intravenous cyclophosphamide, oral cyclophosphamide, or oral azathioprine plus cyclophosphamide had significantly better preservation of renal function than did patients who were randomized to receive prednisone only. Results in the azathioprine group did not differ from those in the prednisone-only group. Cyclophosphamide appears to have long-term benefit in the delay or prevention of end-stage renal disease in patients with lupus nephritis. C1 NIAMSD,ARTHRITIS & RHEUMATISM BRANCH,BETHESDA,MD. NR 25 TC 284 Z9 291 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD AUG PY 1991 VL 34 IS 8 BP 945 EP 950 DI 10.1002/art.1780340803 PG 6 WC Rheumatology SC Rheumatology GA GA988 UT WOS:A1991GA98800002 PM 1859488 ER PT J AU SIMONS, CG AF SIMONS, CG TI SPECIFYING THE CORRECT BIOLOGICAL SAFETY CABINET SO ASHRAE JOURNAL-AMERICAN SOCIETY OF HEATING REFRIGERATING AND AIR-CONDITIONING ENGINEERS LA English DT Article RP SIMONS, CG (reprint author), NIH,DEPT ENGN SERV,DESIGN & CONSTRUCT BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HEAT REFRIG AIR- CONDITIONING ENG INC PI ATLANTA PA 1791 TULLIE CIRCLE NE, ATLANTA, GA 30329 SN 0001-2491 J9 ASHRAE J JI ASHRAE J.-Am. Soc. Heat Refrig. Air-Cond. Eng. PD AUG PY 1991 VL 33 IS 8 BP 31 EP 34 PG 4 WC Thermodynamics; Construction & Building Technology; Engineering, Mechanical SC Thermodynamics; Construction & Building Technology; Engineering GA GB917 UT WOS:A1991GB91700011 ER PT J AU BROWN, VJ BOWMAN, EM ROBBINS, TW AF BROWN, VJ BOWMAN, EM ROBBINS, TW TI RESPONSE-RELATED DEFICITS FOLLOWING UNILATERAL LESIONS OF THE MEDIAL AGRANULAR CORTEX OF THE RAT SO BEHAVIORAL NEUROSCIENCE LA English DT Article ID FRONTAL EYE-FIELD; SUPPLEMENTARY MOTOR AREA; CORTICAL PROJECTIONS; AFFERENT CONNECTIONS; DOPAMINE DEPLETION; PREFRONTAL CORTEX; ROSTRAL PORTION; NEGLECT; ATTENTION; ORGANIZATION AB Rats with lesions of the medial agranular frontal cortex (AGm) were tested for sensorimotor function. Spatial response bias and reaction time to lateralized visual targets were recorded in an automated test of visual reaction time. The same rats were also tested for somatosensory capacity and on a skilled reaching task. In all tasks, there was an ipsilateral response bias but no evidence of sensory neglect. In the visual reaction time task, initiation time was lengthened bilaterally. These deficits may parallel the effects on motor function after unilateral frontal cortical lesioning in primates. The results support the hypothesis that rat AGm contains a homologue of primate secondary motor cortex. C1 UNIV CAMBRIDGE,DEPT EXPTL PSYCHOL,CAMBRIDGE,ENGLAND. RP BROWN, VJ (reprint author), NEI,SENSORIMOTOR RES LAB,BLDG 10,ROOM 10C101,BETHESDA,MD 20892, USA. RI Bowman, Eric/A-3780-2010; Brown, Verity/A-5235-2011 OI Brown, Verity/0000-0001-5762-1797 FU NEI NIH HHS [I F32 EY016180-01]; Wellcome Trust NR 60 TC 40 Z9 40 U1 0 U2 1 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0735-7044 J9 BEHAV NEUROSCI JI Behav. Neurosci. PD AUG PY 1991 VL 105 IS 4 BP 567 EP 578 DI 10.1037/0735-7044.105.4.567 PG 12 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA FZ602 UT WOS:A1991FZ60200008 PM 1930725 ER PT J AU REVELLI, JP PESCINI, R MUZZIN, P SEYDOUX, J FITZGERALD, MG FRASER, CM GIACOBINO, JP AF REVELLI, JP PESCINI, R MUZZIN, P SEYDOUX, J FITZGERALD, MG FRASER, CM GIACOBINO, JP TI CHANGES IN BETA-1-ADRENERGIC AND BETA-2-ADRENERGIC RECEPTOR MESSENGER-RNA LEVELS IN BROWN ADIPOSE-TISSUE AND HEART OF HYPOTHYROID RATS SO BIOCHEMICAL JOURNAL LA English DT Article ID BETA-ADRENERGIC RECEPTORS; THYROID-HORMONE RECEPTOR; ADENYLATE-CYCLASE; SEQUENCE-ANALYSIS; THERMOGENESIS; GENE; GLUCOCORTICOIDS; STIMULATION; EXPRESSION; CLONING AB The aim of the present work was to study the effect of hypothyrodism on the expression of the beta-adrenergic receptor (beta-AR) in interscapular brown adipose tissue and heart. The total density of plasma membrane beta-AR per tissue is decreased by 44% in hypothyroid rat interscapular brown adipose tissue and by 55% in hypothyroid rat heart compared with euthyroid controls. The effects of hypothyroidism on the density of both beta-1- and beta-2-AR subtypes were also determined in competition displacement experiments. The densities of beta-1- and beta-2-AR per tissue are decreased by 50% and 48% respectively in interscapular brown adipose tissue and by 52% and 54% in the heart. Northern blot analysis of poly(A)+ RNA from hypothyroid rat interscapular brown adipose tissue demonstrated that the levels of beta-1- and beta-2-AR mRNA per tissue are decreased by 73 % and 58 % respectively, whereas in hypothyroid heart, only the beta-1-AR mRNA is decreased, by 43 %. The effect of hypothyroidism on the beta-1-AR mRNA is significantly more marked in the interscapular brown adipose tissue than in the heart. These results indicate that beta-AR mRNA levels are differentially regulated in rat interscapular brown adipose tissue and heart, and suggest that the decrease in beta-AR number in interscapular brown adipose tissue and heart of hypothyroid animals may in part be explained by a decreased steady-state level of beta-AR mRNA. C1 CTR MED UNIV GENEVA,DEPT PHYSIOL,CH-1211 GENEVA 4,SWITZERLAND. NINCDS,RECEPTOR BIOCHEM SECT,MOLEC & CELLULAR NEUROBIOL LAB,BETHESDA,MD 20892. NIAAA,MOLEC NEUROBIOL SECT,PHYSIOL & PHARMACOL STUDIES LAB,ROCKVILLE,MD 20852. RP REVELLI, JP (reprint author), CTR MED UNIV GENEVA,DEPT BIOCHIM MED,1 RUE MICHEL SERVET,CH-1211 GENEVA 4,SWITZERLAND. NR 48 TC 38 Z9 39 U1 0 U2 0 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD AUG 1 PY 1991 VL 277 BP 625 EP 629 PN 3 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA372 UT WOS:A1991GA37200008 PM 1651697 ER PT J AU LEE, H CHU, TM LI, SSL LEE, CL AF LEE, H CHU, TM LI, SSL LEE, CL TI HOMODIMER AND HETERODIMER SUBUNITS OF HUMAN PROSTATE ACID-PHOSPHATASE SO BIOCHEMICAL JOURNAL LA English DT Article ID RABBIT SKELETAL-MUSCLE; PROTEIN PHOSPHATASE; PURIFICATION; PHOSPHOMONOESTERASE; HETEROGENEITY; SEQUENCE; GLAND; CDNA AB Human prostatic acid phosphatase (PAP) isoenzymes, designated PAP-A and PAP-B, were isolated from human seminal plasma by sequential affinity chromatography on concanavalin A and L(+)-tartrate, a classic inhibitor of PAP. Both the major PAP-A and the minor PAP-B isoenzymes exhibited a similar molecular mass (100 and 105 kDa respectively), multiple pI values (5.05-5.35 and 5.05-5.12), and substrate and inhibitor specificity. Immunological characterization revealed that PAP-B possesses distinct antigenic determinants, in addition to the common sites shared with PAP-A. SDS/PAGE indicated that both isoenzymes are composed of two subunits of 50 kDa each. At high salt concentration, PAP-B dissociated completely into single subunits of 50 kDa, whereas PAP-A remained intact at 100 kDa. PAP-B was resolved by reverse-phase h.p.l.c. into three components, designated alpha, beta and gamma, each of 50 kDa, at a molar ratio of approx. 2:1:1. PAP-A contained a single component of molecular mass 50 kDa. The single component of PAP-A and the alpha-component of PAP-B possessed identical amino acid compositions and N-terminal sequences, which were different from those of the beta and gamma-components. These results indicate that human PAP contains three isoforms, alpha-2, alpha-beta and alpha-gamma. PAP-A, the major isoenzyme, is a homodimer consisting of two identical subunits (alpha-2), and PAP-B, the minor isoenzyme, is a mixture of two heterodimers, consisting of non-identical subunits (alpha-beta and alpha-gamma). C1 NEW YORK STATE DEPT HLTH,ROSWELL PK MEM INST,DEPT DIAGNOST IMMUNOL RES & BIOCHEM,BUFFALO,NY 14263. NIEHS,GENET LAB,RES TRIANGLE PK,NC 27709. NR 37 TC 17 Z9 18 U1 0 U2 1 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD AUG 1 PY 1991 VL 277 BP 759 EP 765 PN 3 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA372 UT WOS:A1991GA37200030 PM 1908222 ER PT J AU MASUNO, H SCHULTZ, CJ PARK, JW BLANCHETTEMACKIE, EJ MATEO, C SCOW, RO AF MASUNO, H SCHULTZ, CJ PARK, JW BLANCHETTEMACKIE, EJ MATEO, C SCOW, RO TI GLYCOSYLATION, ACTIVITY AND SECRETION OF LIPOPROTEIN-LIPASE IN CULTURED BROWN ADIPOCYTES OF NEWBORN MICE - EFFECT OF TUNICAMYCIN, MONENSIN, 1-DEOXYMANNOJIRIMYCIN AND SWAINSONINE SO BIOCHEMICAL JOURNAL LA English DT Article ID INTRACELLULAR-TRANSPORT; MOUSE ADIPOCYTES; ADIPOSE-CELLS; GOLGI-COMPLEX; KIDNEY-CELLS; BIOSYNTHESIS; PROTEINS; METABOLISM; INHIBITION; INSULIN AB The effect of inhibitors on the glycosylation, activity and secretion of lipoprotein lipase was studied in brown adipocytes cultured from newborn mice. Such cells synthesized and secreted active lipoprotein lipase. It is generally accepted that active lipoprotein lipase is a homodimer. Glycosylation of lipoprotein lipase was analysed by PAGE of endoglycosidase H (endo H)-digested subunits of lipoprotein lipase immunoprecipitated from cells incubated for 1-2 h with [S-35]methionine. The most prevalent S-35-labelled lipase subunit (M(r) 57000-58000) in these cells contained endo H-resistant oligosaccharide chains, the next most prevalent contained totally endo H-sensitive chains, and the least prevalent subunit contained partially endo H-sensitive chains. Complete blocking of the glycosylation of lipoprotein lipase with tunicamycin (1-mu-g/ml) for 24 h resulted in synthesis of an inactive non-secretable form of lipase with a smaller subunit (M(r) 51000-52000). Immunofluorescent studies showed that unglycosylated lipase in tunicamycin-treated cells was retained in the endoplasmic reticulum. Cells treated with 1-mu-M-monensin, an intra-Golgi transport inhibitor, synthesized an active form of lipase which was not secreted, but was retained in the Golgi. The lipase in monensin-treated cells contained only partially or totally endo H-sensitive chains. Blocking either Golgi mannosidase I with 4 mM-1-deoxymannojirimycin or Golgi mannosidase II with 10-mu-M-swainsonine resulted in production of a form of lipoprotein lipase which was active and secreted, and which contained only endo H-sensitive chains. Our findings demonstrate that core glycosylation of lipoprotein lipase in the endoplasmic reticulum is required for lipase activity and transport from the reticulum, whereas processing of the oligosaccharide chains to endo H-resistant (complex) type chains in the Golgi is not required for either the activity or the secretion of lipoprotein lipase. C1 NIDDKD,ENDOCRINOL SECT,CELLULAR & DEV BIOL LAB,BLDG 6,ROOM 137,BETHESDA,MD 20892. NR 52 TC 45 Z9 46 U1 0 U2 1 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD AUG 1 PY 1991 VL 277 BP 801 EP 809 PN 3 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA372 UT WOS:A1991GA37200036 PM 1831351 ER PT J AU MAURIZI, MR AF MAURIZI, MR TI ATP-PROMOTED INTERACTION BETWEEN CLP-A AND CLP-P IN ACTIVATION OF CLP PROTEASE FROM ESCHERICHIA-COLI SO BIOCHEMICAL SOCIETY TRANSACTIONS LA English DT Article; Proceedings Paper CT 638TH MEETING OF THE BIOCHEMICAL SOC CY APR 10-12, 1991 CL READING UNIV, READING, ENGLAND SP BIOCHEM SOC HO READING UNIV ID DEPENDENT PROTEASE; LA; HYDROLYSIS; PEPTIDES; GENE; TI; COMPONENT; BREAKDOWN; SEQUENCE; CONTAINS AB Clp protease is a high relative molecular mass, ATP-dependent protease found in the cytoplasm of Escherichia coli. Clp protease is composed of two protein components, Clp A, which has ATPase activity, and Clp P, which has the proteolytic active site and is activated by Clp A in the presence of ATP. Clp P subunits (M(r) = 21 500) are arranged in two hexagonal rings directly superimposed on each other, and under low salt conditions two dodecamers associate to form a particle with M(r) approximately 440 000. Clp A (subunit M(r) = 83 000) and Clp P do not associate in the absence of nucleotide, but Clp A with ATP bound associates with Clp P to form an active proteolytic complex with M(r) approximately 700 000. Although adenosine 5'-[beta-gamma-imido]triphosphate (AMPPNP) weakly promotes association between Clp A and Clp P, non-hydrolysable analogues of ATP do not activate proteolysis, indicating that association between the components is not sufficient to allow proteolysis. Association between Clp A and Clp P does not alter the basal ATPase activity of Clp A, but addition of protein substrates is accompanied by an increase in ATP hydrolysis by Clp A. Chemically-inactivated Clp P or inactive mutants of Clp P also associate with Clp A, but no increase in the ATPase activity of Clp A is observed, either in the presence or absence of protein substrates, when Clp P is inactive. Thus the increased ATP hydrolysis is dependent on active proteolysis. These data indicate that ATP has two functions in activating Clp protease: an allosteric role in promoting association between the subunits and a mechanistically undefined role in promoting continuous rounds of peptide bond cleavage. These functions may be separately fulfilled by the two ATP-binding sites identified by sequence analysis on each Clp A subunit. RP MAURIZI, MR (reprint author), NCI,CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 20 TC 47 Z9 47 U1 0 U2 1 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0300-5127 J9 BIOCHEM SOC T JI Biochem. Soc. Trans. PD AUG PY 1991 VL 19 IS 3 BP 719 EP 723 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GE101 UT WOS:A1991GE10100038 PM 1783205 ER PT J AU AKHMALOKA GRANT, CM STANSFIELD, I TUITE, MF AF AKHMALOKA GRANT, CM STANSFIELD, I TUITE, MF TI THE USE OF ALLOSUPPRESSOR ALLELES OF THE SAL4 GENE IN THE STUDY OF TRANSLATIONAL FIDELITY IN SACCHAROMYCES-CEREVISIAE SO BIOCHEMICAL SOCIETY TRANSACTIONS LA English DT Meeting Abstract ID YEAST; SUP45 C1 UNIV KENT,BIOL LAB,CANTERBURY CT2 7NJ,KENT,ENGLAND. NIH,BETHESDA,MD 20892. NR 6 TC 0 Z9 0 U1 0 U2 0 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0300-5127 J9 BIOCHEM SOC T JI Biochem. Soc. Trans. PD AUG PY 1991 VL 19 IS 3 BP S281 EP S281 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GE101 UT WOS:A1991GE10100095 PM 1783125 ER PT J AU MICKISCH, GH PASTAN, I GOTTESMAN, MM AF MICKISCH, GH PASTAN, I GOTTESMAN, MM TI MULTIDRUG RESISTANT TRANSGENIC MICE AS A NOVEL PHARMACOLOGICAL TOOL SO BIOESSAYS LA English DT Review ID DEPENDENT TRANSPORT; P-GLYCOPROTEIN; TUMOR-CELLS; GENE; EXPRESSION; PROTEIN; LOCALIZATION; MDR1; VINBLASTINE; MEMBRANE AB Multidrug resistance resulting from expression of an energy-dependent drug efflux pump encoded by the human MDR1 gene is a major impediment to effective cancer therapy. Pharmacologic intervention aimed at inhibiting this multidrug transporter should improve existing chemotherapy of human cancer, but drug development has been delayed by the difficulty and expense of developing valid animal models. Using recombinant DNA technology, a transgenic mouse has been engineered whose bone marrow is protected from the toxic effects of chemotherapy by expression of the MDR1 gene. This animal system allows the rapid screening of drugs which inhibit the multidrug transporter and heralds a new era of using transgenic animals for pharmacologic screening. C1 NCI,DCBDC,CELL BIOL LAB,BETHESDA,MD 20892. NCI,DCBDC,CELL BIOL LAB,BETHESDA,MD 20892. NR 22 TC 20 Z9 21 U1 0 U2 0 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL SN 0265-9247 J9 BIOESSAYS JI Bioessays PD AUG PY 1991 VL 13 IS 8 BP 381 EP 387 DI 10.1002/bies.950130804 PG 7 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GC690 UT WOS:A1991GC69000002 PM 1683231 ER PT J AU CARRINGTON, JL REDDI, AH AF CARRINGTON, JL REDDI, AH TI PARALLELS BETWEEN DEVELOPMENT OF EMBRYONIC AND MATRIX-INDUCED ENDOCHONDRAL BONE SO BIOESSAYS LA English DT Article ID LIMB-BUD CHONDROGENESIS; GROWTH FACTOR-BETA; DIFFERENTIATION INVIVO; MORPHOGENETIC PROTEIN; CELL-PROLIFERATION; CARTILAGE; COLLAGEN; INVITRO; FIBRONECTIN; PATTERNS AB Endochondral bone formation can take place in the embryo, during fracture healing, or in postnatal animals after induction by implanted demineralized bone matrix. This matrix-induced bone formation recapitulates the embryonic sequence of bone formation morphologically and biochemically. The steps in bone formation in both systems include differentiation of cartilage from mesenchyme, cartilage maturation, invasion of the cartilage by blood vessels and marrow precursors, and formation of bone and bone marrow. Recently, bone inductive molecules from demineralized bone matrix have been purified, sequenced and produced as recombinant proteins. While there are similarities between bone development in the embryo and that after induction by these purified molecules, the molecules responsible for bone induction in the induction in the embryo have not yet been defined. Because of similarities between the two methods of bone formation, studies of bone induction by demineralized bone matrix may help to elucidate mechanisms of embryonic bone induction. C1 NIDR,BONE CELL BIOL SECT,BLDG 30,ROOM 211,BETHESDA,MD 20892. UNIFORMED SERV UNIV HLTH SCI,DEPT ANAT,F EDWARD HEBERT SCH MED,BETHESDA,MD 20814. NR 40 TC 32 Z9 32 U1 0 U2 3 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL SN 0265-9247 J9 BIOESSAYS JI Bioessays PD AUG PY 1991 VL 13 IS 8 BP 403 EP 408 DI 10.1002/bies.950130807 PG 6 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GC690 UT WOS:A1991GC69000005 PM 1953701 ER PT J AU PARDHASARADHI, K LUDWIG, B HENDLER, RW AF PARDHASARADHI, K LUDWIG, B HENDLER, RW TI POTENTIOMETRIC AND SPECTRAL STUDIES WITH THE 2-SUBUNIT CYTOCHROME AA3 FROM PARACOCCUS-DENITRIFICANS - COMPARISON WITH THE 13-SUBUNIT BEEF-HEART ENZYME SO BIOPHYSICAL JOURNAL LA English DT Article ID C-OXIDASE; REDOX PROPERTIES; MITOCHONDRIA; COMPONENTS; TERMS AB Previous work from this laboratory has revealed a complex and interactive redox behavior for the active metal centers in beef heart cytochrome aa3. All of these centers are contained in two of the 13 subunits which make up the enzyme. The isolated cytochrome aa3 of Paracoccus denitrificans contains only two subunits. The purpose of the current investigation was to see if the complex redox behavior is dependent on the presence of the additional 11 peptides that are present in the mammalian enzyme. In this paper we report that the structurally simpler bacterial enzyme displays a redox behavior which is very similar to that seen with the mammalian enzyme. Therefore, the observed redox behavior does not depend on interactions involving the additional peptides. C1 MED UNIV LUBECK,INST BIOCHEM,LUBECK,GERMANY. RP PARDHASARADHI, K (reprint author), NHLBI,CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 12 TC 23 Z9 23 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD AUG PY 1991 VL 60 IS 2 BP 408 EP 414 PG 7 WC Biophysics SC Biophysics GA FY762 UT WOS:A1991FY76200013 PM 1655082 ER PT J AU HENDLER, RW PARDHASARADHI, K REYNAFARJE, B LUDWIG, B AF HENDLER, RW PARDHASARADHI, K REYNAFARJE, B LUDWIG, B TI COMPARISON OF ENERGY-TRANSDUCING CAPABILITIES OF THE 2-SUBUNIT AND 3-SUBUNIT CYTOCHROMES AA3 FROM PARACOCCUS-DENITRIFICANS AND THE 13-SUBUNIT BEEF-HEART ENZYME SO BIOPHYSICAL JOURNAL LA English DT Article ID C-OXIDASE ACTIVITY; SUBUNIT-III; PROTON TRANSLOCATION; ELECTRON-TRANSFER; OXYGEN-UPTAKE; PH; VESICLES; PROTEOLIPOSOMES; RESPIRATION; COMPLEXES AB In the accompanying paper, we have shown that the two-subunit cytochrome aa3 isolated from Paracoccus denitrificans displays the same kind of complex and interactive redox behavior as the 13-subunit cytochrome aa3 from beef heart. Therefore, the redox characteristics are not dependent on the additional 11 subunits. In the current work, we have examined the energy-transducing capabilities of both the two- and three-subunit enzymes obtained from Paracoccus denitrificans in relation to that of the 13-unit mammalian enzyme. We have found that in all of the tested functions, which included the development of DELTA-PSI and DELTA-pH, and the pumping of protons, that the two-subunit enzyme is at least as efficient as the structurally more complex mammalian enzyme. There is thus a correlation between the complex redox behavior and energy transducing capabilities of the two enzymes. There was also no difference in energy-transducing capabilities between the two- and three-subunit forms of the bacterial enzyme. It seems that only 2 subunits are required for an efficient energy-transducing cytochrome aa3. The most likely role of the additional subunits in the mammalian enzyme, therefore, seems to be in regulation. C1 MED UNIV LUBECK,INST BIOCHEM,LUBECK,GERMANY. RP HENDLER, RW (reprint author), NHLBI,CELLULAR BIOL LAB,BETHESDA,MD 20892, USA. NR 33 TC 97 Z9 97 U1 0 U2 2 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD AUG PY 1991 VL 60 IS 2 BP 415 EP 423 PG 9 WC Biophysics SC Biophysics GA FY762 UT WOS:A1991FY76200014 PM 1655083 ER PT J AU SAROFF, HA AF SAROFF, HA TI IONIZATION OF CLUSTERS .4. COOPERATIVITY AND ALLOSTERISM DERIVED FROM SHARED CLUSTERS IN MACROMOLECULES SO BIOPOLYMERS LA English DT Article ID BINDING DATA; HEMOGLOBIN AB Clusters of ionizable groups are examined for conditions that develop cooperativity (1) on the binding of protons, and (2) on the binding of an associated ligand when the clusters are shared between domains or subunits in macromolecules. Cooperative binding isotherms for protons have long been observed (but not emphasized as cooperative binding) when studies have been done on clusters for the evaluation of metal ion complexation [A. E. Martell & M. Calvin (1952) Chemistry of the Metal Chelate Compounds, Prentice-Hall, Englewood Cliffs, New Jersey]. Reactions are formulated in this paper to show that anions, chelating to positively charged clusters, are also capable of developing the cooperative binding of protons. Extension of these simple reactions to those where clusters of ionizable groups are shared between domains of macromolecules provides models for cooperative binding, which include the allosteric, Bohr, anion, and cation effects in proteins. RP SAROFF, HA (reprint author), NIDDKD,BIOCHEM PHARMACOL LAB,BLDG 8,ROOM 227,BETHESDA,MD 20892, USA. NR 20 TC 1 Z9 1 U1 0 U2 0 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0006-3525 J9 BIOPOLYMERS JI Biopolymers PD AUG PY 1991 VL 31 IS 9 BP 1037 EP 1047 DI 10.1002/bip.360310904 PG 11 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GG112 UT WOS:A1991GG11200003 PM 1664745 ER PT J AU MERLO, GR CROPP, CS CALLAHAN, R TAKAHASHI, T AF MERLO, GR CROPP, CS CALLAHAN, R TAKAHASHI, T TI DETECTION OF LOSS OF HETEROZYGOSITY IN TUMOR DNA SAMPLES BY PCR SO BIOTECHNIQUES LA English DT Note ID GENE; POLYMORPHISM; CANCER AB We demonstrate that PCR amplification of human genomic DNA can be used for the detection of loss of heterozygosity (LOH) in tumor samples. A 250-bp fragment containing codon 72 of the human p53 gene was amplified, ThaI digested and electrophoresed. Tumor LOH is detectable both by ethidium bromide staining and autoradiography, despite 25% contamination with normal DNA. This technique provides a fast and reproducible alternative to conventional Southern blotting and has minimal sample requirements. C1 NCI,TUMOR IMMUNOL & BIOL LAB,ONCOGENET SECT,BLDG 10,ROOM 5B50,BETHESDA,MD 20892. USN HOSP,NCI,MED ONCOL BRANCH,BETHESDA,MD 20892. RI Takahashi, Takashi/I-7262-2014 NR 12 TC 24 Z9 25 U1 0 U2 0 PU EATON PUBLISHING CO PI NATICK PA 154 E. CENTRAL ST, NATICK, MA 01760 SN 0736-6205 J9 BIOTECHNIQUES JI Biotechniques PD AUG PY 1991 VL 11 IS 2 BP 166 EP & PG 0 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GB875 UT WOS:A1991GB87500004 PM 1931011 ER PT J AU GOLDSTEIN, BM LEARY, JF FARLEY, BA MARQUEZ, VE LEVY, PC ROWLEY, PT AF GOLDSTEIN, BM LEARY, JF FARLEY, BA MARQUEZ, VE LEVY, PC ROWLEY, PT TI INDUCTION OF HL-60 CELL-DIFFERENTIATION BY TIAZOFURIN AND ITS ANALOGS - CHARACTERIZATION AND EFFICACY SO BLOOD LA English DT Article ID THIAZOLE-4-CARBOXAMIDE ADENINE-DINUCLEOTIDE; DEHYDROGENASE-INHIBITORY PROPERTIES; INOSINE 5'-PHOSPHATE DEHYDROGENASE; ACUTE MYELOID-LEUKEMIA; IMP DEHYDROGENASE; MONOCLONAL-ANTIBODIES; THIAZOLE NUCLEOSIDE; MYCOPHENOLIC-ACID; IMMUNOGLOBULIN-G; FC-RECEPTORS C1 UNIV ROCHESTER,SCH MED,DIV GENET,POB 641,ROCHESTER,NY 14642. UNIV ROCHESTER,SCH MED,DEPT BIOPHYS,ROCHESTER,NY 14642. UNIV ROCHESTER,SCH MED,DEPT MED,ROCHESTER,NY 14642. UNIV ROCHESTER,SCH MED,DEPT PATHOL & LAB MED,ROCHESTER,NY 14642. NCI,BETHESDA,MD 20892. FU NCI NIH HHS [CA-45145] NR 54 TC 60 Z9 60 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD AUG 1 PY 1991 VL 78 IS 3 BP 593 EP 598 PG 6 WC Hematology SC Hematology GA FZ566 UT WOS:A1991FZ56600008 PM 1650262 ER PT J AU MATSUSHIME, H ROUSSEL, MF MATSUSHIMA, K HISHINUMA, A SHERR, CJ AF MATSUSHIME, H ROUSSEL, MF MATSUSHIMA, K HISHINUMA, A SHERR, CJ TI CLONING AND EXPRESSION OF MURINE INTERLEUKIN-1 RECEPTOR ANTAGONIST IN MACROPHAGES STIMULATED BY COLONY-STIMULATING FACTOR-I SO BLOOD LA English DT Article ID FACTOR-I RECEPTOR; MOUSE MYELOID CELLS; C-FMS GENE; GROWTH-FACTOR; CSF-1 RECEPTOR; V-FMS; IL-1 RECEPTOR; HIGH-AFFINITY; T-CELLS; DIFFERENTIATION C1 ST JUDE CHILDRENS RES HOSP, HOWARD HUGHES MED INST, DEPT TUMOR CELL BIOL, 332 N LAUDERDALE, MEMPHIS, TN 38105 USA. UNIV TENNESSEE, CTR HLTH SCI, COLL MED, DEPT BIOCHEM, MEMPHIS, TN 38163 USA. NCI, FREDERICK CANC RES FACIL, IMMUNOREGULAT LAB, FREDERICK, MD 21701 USA. FU NCI NIH HHS [CA47064, CA21765] NR 57 TC 31 Z9 33 U1 0 U2 0 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 1900 M STREET. NW SUITE 200, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD AUG 1 PY 1991 VL 78 IS 3 BP 616 EP 623 PG 8 WC Hematology SC Hematology GA FZ566 UT WOS:A1991FZ56600012 PM 1830498 ER PT J AU BASTA, M FRIES, LF FRANK, MM AF BASTA, M FRIES, LF FRANK, MM TI HIGH-DOSES OF INTRAVENOUS IMMUNOGLOBULIN DO NOT AFFECT THE RECOGNITION PHASE OF THE CLASSICAL COMPLEMENT PATHWAY SO BLOOD LA English DT Article ID ACTIVATION; MECHANISM; C1 C1 JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,CTR IMMUNIZAT RES,BALTIMORE,MD 21218. RP BASTA, M (reprint author), NIAID,CLIN INVEST LAB,BLDG 10,ROOM 11N250,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. OI Basta, Milan/0000-0001-5958-9241 NR 11 TC 24 Z9 24 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD AUG 1 PY 1991 VL 78 IS 3 BP 700 EP 702 PG 3 WC Hematology SC Hematology GA FZ566 UT WOS:A1991FZ56600022 PM 1859883 ER PT J AU SPIRTAS, R STEWART, PA LEE, JS MARANO, DE FORBES, CD GRAUMAN, DJ PETTIGREW, HM BLAIR, A HOOVER, RN COHEN, JL AF SPIRTAS, R STEWART, PA LEE, JS MARANO, DE FORBES, CD GRAUMAN, DJ PETTIGREW, HM BLAIR, A HOOVER, RN COHEN, JL TI RETROSPECTIVE COHORT MORTALITY STUDY OF WORKERS AT AN AIRCRAFT MAINTENANCE FACILITY .1. EPIDEMIOLOGIC RESULTS SO BRITISH JOURNAL OF INDUSTRIAL MEDICINE LA English DT Article ID NON-HODGKINS LYMPHOMA; PRIMARY LIVER-CANCER; DRY CLEANING WORKERS; BREAST-CANCER; ALCOHOL-CONSUMPTION; METHYLENE-CHLORIDE; OCCUPATIONAL EXPOSURE; ORGANIC-SOLVENTS; RISK-FACTORS; TRICHLOROETHYLENE AB A retrospective cohort study of 14 457 workers at an aircraft maintenance facility was undertaken to evaluate mortality associated with exposures in their workplace. The purpose was to determine whether working with solvents, particularly trichloroethylene, posed any excess risk of mortality. The study group consisted of all civilian employees who worked for at least one year at Hill Air Force Base, Utah, between 1 January 1952 and 31 December 1956. Work histories were obtained from records at the National Personnel Records Centre, St. Louis, Missouri, and the cohort was followed up for ascertainment of vital state until 31 December 1982. Observed deaths among white people were compared with the expected number of deaths, based on the Utah white population, and adjusted for age, sex, and calendar period. Significant deficits occurred for mortality from all causes (SMR 92, 95% confidence interval (95% CI) 90-95), all malignant neoplasms (SMR 90, 95% CI 83-97), ischaemic heart disease (SMR 93, 95% CI 88-98), non-malignant respiratory disease (SMR 87, 95% CI 76-98), and accidents (SMR 61, 95% CI 52-70). Mortality was raised for multiple myeloma (MM) in white women (SMR 236, 95% CI 87-514), non-Hodgkin's lymphoma (NHL) in white women (SMR 212, 95% CI 102-390), and cancer of the biliary passages and liver in white men dying after 1980 (SMR 358, 95% CI 116-836). Detailed analysis of the 6929 employees occupationally exposed to trichloroethylene, the most widely used solvent at the base during the 1950s and 1960s, did not show any significant or persuasive association between several measures of exposure to trichloroethylene and any excess of cancer. Women employed in departments in which fabric cleaning and parachute repair operations were performed had more deaths than expected from MM and NHL. The inconsistent mortality patterns by sex, multiple and overlapping exposures, and small numbers made it difficult to ascribe these excesses to any particular substance. Hypothesis generating results are presented by a variety of exposures for causes of death not showing excesses in the overall cohort. C1 NCI,BETHESDA,MD 20892. UNIV UTAH,SALT LAKE CITY,UT 84112. WESTAT CORP,ROCKVILLE,MD. NR 73 TC 126 Z9 127 U1 0 U2 1 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON, ENGLAND WC1H 9JR SN 0007-1072 J9 BRIT J IND MED PD AUG PY 1991 VL 48 IS 8 BP 515 EP 530 PG 16 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FZ498 UT WOS:A1991FZ49800004 PM 1878308 ER PT J AU STEWART, PA LEE, JS MARANO, DE SPIRTAS, R FORBES, CD BLAIR, A AF STEWART, PA LEE, JS MARANO, DE SPIRTAS, R FORBES, CD BLAIR, A TI RETROSPECTIVE COHORT MORTALITY STUDY OF WORKERS AT AN AIRCRAFT MAINTENANCE FACILITY .2. EXPOSURES AND THEIR ASSESSMENT SO BRITISH JOURNAL OF INDUSTRIAL MEDICINE LA English DT Article AB Methods are presented that were used for assessing exposures in a cohort mortality study of 15 000 employees who held 150 000 jobs at an Air Force base from 1939 to 1982. Standardisation of the word order and spelling of the job titles identified 43 000 unique job title organisation combinations. Walkthrough surveys were conducted, long term employees were interviewed, and available industrial hygiene data were collected to evaluate historic exposures. Because of difficulties linking air monitoring data and use of specific chemicals to the departments identified in the work histories, position descriptions were used to identify the tasks in each job. From knowledge of the tasks and the chemicals used in those tasks the presence or absence of 23 chemicals or groups of chemicals were designated for each job organisation combination. Also, estimates of levels of exposure were made for trichloroethylene and for mixed solvents, a category comprising several solvents including trichloroethylene, Stoddard solvent, carbon tetrachloride, JP4 gasoline, freon, alcohols, 1,1,1-trichloroethane, acetone, toluene, methyl ethyl ketone, methylene chloride, o-dichlorobenzene, perchloroethylene, chloroform, styrene, and xylene. C1 NCI,BETHESDA,MD 20892. UNIV UTAH,SALT LAKE CITY,UT 84112. WESTAT CORP,ROCKVILLE,MD. NR 21 TC 41 Z9 41 U1 0 U2 1 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON, ENGLAND WC1H 9JR SN 0007-1072 J9 BRIT J IND MED PD AUG PY 1991 VL 48 IS 8 BP 531 EP 537 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FZ498 UT WOS:A1991FZ49800005 PM 1878309 ER PT J AU CASCELLA, NG PEARLSON, G WONG, DF BROUSSOLLE, E NAGOSHI, C MARGOLIN, RA LONDON, ED AF CASCELLA, NG PEARLSON, G WONG, DF BROUSSOLLE, E NAGOSHI, C MARGOLIN, RA LONDON, ED TI EFFECTS OF SUBSTANCE-ABUSE ON VENTRICULAR AND SULCAL MEASURES ASSESSED BY COMPUTERIZED-TOMOGRAPHY SO BRITISH JOURNAL OF PSYCHIATRY LA English DT Article; Proceedings Paper CT 50TH ANNUAL MEETING OF THE COMMITTEE ON PROBLEMS OF DRUG DEPENDENCE CY JUN 28-30, 1988 CL NORTH FALMOUTH, MA SP COMM PROBLEMS DRUG DEPENDENCE ID AXIAL-TOMOGRAPHY; BRAIN-DAMAGE; SCHIZOPHRENICS; ADDICTION; ALCOHOL; DENSITY; FLUID; INDEX AB Computerised tomography (CT) was used to assess the possible effects of substance abuse on brain morphology. Polydrug abusers had significantly wider third ventricles than normal controls, with a positive correlation between age and ventricle:brain ratio (VBR). Assuming no effect of age, estimated quantity of substance abuse was not significantly related to ventricular and sulcal measures, except that alcohol consumption correlated positively with VBR and severity of cocaine use correlated negatively with sulcal width. When age of the subjects was partialled out, alcohol use showed a tendency for association with VBR; however, severity of cocaine use did not remain a significant predictor of cortical sulcal width. The findings suggest that chronic use of alcohol, but not necessarily of other commonly abused substances, produces brain atrophy. C1 JOHNS HOPKINS MED INST,DEPT RADIOL,BALTIMORE,MD 21205. JOHNS HOPKINS MED INST,DEPT PSYCHIAT & HYG & PUBL HLTH,BALTIMORE,MD 21205. RP CASCELLA, NG (reprint author), NIDA,ADDICT RES CTR,BALTIMORE,MD, USA. NR 25 TC 18 Z9 18 U1 0 U2 0 PU ROYAL COLLEGE OF PSYCHIATRISTS PI LONDON PA BRITISH JOURNAL OF PSYCHIATRY 17 BELGRAVE SQUARE, LONDON, ENGLAND SW1X 8PG SN 0007-1250 J9 BRIT J PSYCHIAT JI Br. J. Psychiatry PD AUG PY 1991 VL 159 BP 217 EP 221 DI 10.1192/bjp.159.2.217 PG 5 WC Psychiatry SC Psychiatry GA GA581 UT WOS:A1991GA58100007 PM 1773237 ER PT J AU STRONG, MJ GARRUTO, RM AF STRONG, MJ GARRUTO, RM TI CHRONIC ALUMINUM-INDUCED MOTOR-NEURON DEGENERATION - CLINICAL, NEUROPATHOLOGICAL AND MOLECULAR BIOLOGICAL ASPECTS SO CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES LA English DT Article ID AMYOTROPHIC LATERAL SCLEROSIS; EXPERIMENTAL NEUROFIBRILLARY CHANGES; ORALLY-ADMINISTERED ALUMINUM; NEUROFILAMENT PHOSPHORYLATION; CYTOSKELETAL PROTEINS; CHRONIC ANIMALS; CYCLIC-AMP; DISEASE; TOXICITY; NEUROTOXICITY AB The monthly intracisternal inoculation of young adult New Zealand white rabbits with low-dose (100-mu-g) aluminum chloride induces aggregates of phosphorylated neurofilament that mimics the intraneuronal inclusions of amyotrophic lateral sclerosis. The chronic progressive myelopathy and topographically-specific motor neuron degeneration that occurs in the absence of suppressions of neurofilament messenger RNA levels in this model contrasts with the acute fulminant encephalomyelopathy and nonspecific gene suppressions that occur subsequent to high-dose (100-mu-g) aluminum chloride inoculations. Further analysis of this unique model of chronic motor system degeneration can be expected to provide additional insights into the pathogenesis of amyotrophic lateral sclerosis. C1 NIH,CENT NERVOUS SYST STUDIES,BETHESDA,MD 20892. RP STRONG, MJ (reprint author), UNIV WESTERN ONTARIO,DEPT CLIN NEUROL SCI,LONDON N6A 5A5,ONTARIO,CANADA. RI Strong, Michael/H-9689-2012 NR 68 TC 24 Z9 24 U1 0 U2 1 PU CANADIAN J NEUROL SCI INC PI CALGARY PA PO BOX 4220, STATION C EDITORIAL & SUBSCRIPTION SERV, CALGARY AB T2T 5N1, CANADA SN 0317-1671 J9 CAN J NEUROL SCI JI Can. J. Neurol. Sci. PD AUG PY 1991 VL 18 IS 3 SU S BP 428 EP 431 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA GD625 UT WOS:A1991GD62500016 PM 1933693 ER PT J AU KNELLER, RW MCLAUGHLIN, JK BJELKE, E SCHUMAN, LM BLOT, WJ WACHOLDER, S GRIDLEY, G COCHIEN, HT FRAUMENI, JF AF KNELLER, RW MCLAUGHLIN, JK BJELKE, E SCHUMAN, LM BLOT, WJ WACHOLDER, S GRIDLEY, G COCHIEN, HT FRAUMENI, JF TI A COHORT STUDY OF STOMACH-CANCER IN A HIGH-RISK AMERICAN POPULATION SO CANCER LA English DT Article ID GASTRIC-CANCER; ALCOHOL-CONSUMPTION; DIETARY FACTORS; UNITED-STATES; EPIDEMIOLOGY; SMOKING; COUNTY AB Demographic, smoking and dietary information was obtained from a cohort of 17,633 white American men, largely of Scandinavian and German descent, who responded to a mailed questionnaire in 1966. After 20 years of follow-up, 50% to 90% increases in mortality from stomach cancer (75 deaths) were found among foreign-born, their children, and among residents of the North Central states. An association was seen with low educational attainment and laboring or semiskilled occupations, primarily among immigrants and their children. Risk was elevated in subjects who regularly smoked cigarettes (RR = 2.6, 95% CI = 1.1 to 5.8). A significant dose-response trend was observed, with subjects who smoked 30 or more cigarettes per day having more than a five-fold increased risk compared with those who never smoked. Elevated risks were also found for pipe smoking and smokeless tobacco use, but not for alcohol consumption. Analysis of dietary consumption of nine food groups revealed no significant associations with stomach cancer. However, total carbohydrate intake and a few individual food items (salted fish, bacon, cooked cereal, milk, and apples) were associated with increased risk. The findings of this prospective study of a high-risk population add to the limited evidence relating tobacco consumption to stomach cancer risk and suggest clues to ethnic, geographic, and dietary risk factors. C1 NCI,DIV CANC RES,EPIDEMIOL & BIOSTAT PROGRAM,EPN SUITE 415,BETHESDA,MD 20892. WESTAT CORP,ROCKVILLE,MD. UNIV BERGEN,CTR EPIDEMIOL RES,N-5014 BERGEN,NORWAY. UNIV MINNESOTA,SCH PUBL HLTH,DIV EPIDEMIOL,MINNEAPOLIS,MN 55455. FU NCI NIH HHS [N01-CP-33325] NR 44 TC 103 Z9 105 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD AUG 1 PY 1991 VL 68 IS 3 BP 672 EP 678 DI 10.1002/1097-0142(19910801)68:3<672::AID-CNCR2820680339>3.0.CO;2-T PG 7 WC Oncology SC Oncology GA FY161 UT WOS:A1991FY16100038 PM 2065291 ER PT J AU MASUI, T WARD, JM COHEN, SM AF MASUI, T WARD, JM COHEN, SM TI ENHANCED IMMUNOREACTIVITY OF RAS ONCOGENE-P21 PROTEIN IN URINARY-BLADDER EPITHELIUM OF RATS TREATED WITH N-[4-(5-NITRO-2-FURYL)-2-THIAZOLYL] FORMAMIDE SO CANCER LETTERS LA English DT Article DE RAT; URINARY BLADDER; N-[4-(5-NITRO-2-FURYL)-2-THIAZOYL]FORMAMIDE; P21; RAS ONCOGENE ID TRANSITIONAL CELL-CARCINOMA; H-RAS; HUMAN CANCER; GENETIC MECHANISMS; TUMOR INITIATION; CARCINOGENESIS; ACTIVATION; P21; EXPRESSION; LESIONS AB Normal urothelium and various lesions of the rat urinary bladder induced by the dietary administration of 0.2% N-[4-(5-nitro-2-furyl)-2-thiazoyl]formamide (FANFT) (up to 77 weeks) or by the combination of 0.2% FANFT and the subsequent administration of 5% sodium saccharin or 2% DL-tryptophan (up to 104 weeks) were evaluated for immunoreactivity with monoclonal antibody to ras p21 by avidin-biotin immunohistochemistry. Seventy-one to 100% of transitional cell carcinomas showed strong reactivity to the antibody to ras p21 depending on treatment with long-term administration of FANFT or by 6 weeks administration of FANFT followed by sodium saccharin or DL-tryptophan. Focal reactivity to the ras p21 antibody was frequently observed in the hyperplastic (57 - 96%) or normal appearing urinary bladder epithelium (50 - 100%) in rats treated with FANFT (FANFT alone or in combination with sodium saccharin or tryptophan) but not in hyperplasia or normal epithelium in rats given sodium saccharin or tryptophan alone, without pretreatment with FANFT or in untreated controls. The present results show that there is a close association of enhanced immunoreactivity with ras p21 antibody in the urinary bladder epithelium to FANFT treatment, and that ras p21 is expressed in normal, hyperplastic and neoplastic lesions of the bladder of rats treated with FANFT. These results suggest that enhanced immunoreactivity with ras p21 is observed as a consequence of the treatment with FANFT but it alone does not reflect the progression from benign to malignant lesions. C1 UNIV NEBRASKA,MED CTR,DEPT PATHOL & MICROBIOL,600 S 42ND ST,OMAHA,NE 68198. UNIV NEBRASKA,MED CTR,EPPLEY INST RES CANC & ALLIED DIS,OMAHA,NE 68198. NCI,DIV CANC ETIOL,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21701. FU NCI NIH HHS [CA36727, CA32513] NR 32 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3835 J9 CANCER LETT JI Cancer Lett. PD AUG PY 1991 VL 59 IS 2 BP 95 EP 102 DI 10.1016/0304-3835(91)90172-E PG 8 WC Oncology SC Oncology GA GC841 UT WOS:A1991GC84100003 PM 1884376 ER PT J AU COLEMAN, EA PENNYPACKER, H AF COLEMAN, EA PENNYPACKER, H TI MEASURING BREAST SELF-EXAMINATION PROFICIENCY - A SCORING SYSTEM DEVELOPED FROM A PAIRED COMPARISON STUDY SO CANCER NURSING LA English DT Article DE BREAST SELF-EXAMINATION; MEASURING PROFICIENCY; BSE SCORING SYSTEM AB A continuous scoring strategy yielding a proficiency score on breast self-examination (BSE) performance was developed and validated. The method of paried comparisons was used to provide a rank ordering on an interval scale of the eight components of the evaluation tool. Twenty-one experts in teaching the MammaCare method of breast self-examination participated in the survey to help in developing the composite measure of proficiency in BSE performance. Data on eachcomponent of BSE were collected quantitatively, and performance scores were computed using a formula incorporating the weighting schema derived from the paired comparisons procedure. A comparison of preteaching versus postteaching mean performance scores on BSE indicated that the scoring system worked even when the teaching method varied. The scoring system presented here is a start on the development of a tool that can be useful to researchers using relative performance as a measure in BSE studies. The components of the scoring system can be used by nurses in the practice setting as a checklist for evaluating BSE performance. RP COLEMAN, EA (reprint author), NCI, EPN 343J, BETHESDA, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0162-220X EI 1538-9804 J9 CANCER NURS JI Cancer Nurs. PD AUG PY 1991 VL 14 IS 4 BP 211 EP 217 DI 10.1097/00002820-199114040-00007 PG 7 WC Oncology; Nursing SC Oncology; Nursing GA GA363 UT WOS:A1991GA36300007 PM 1913636 ER PT J AU LEHMAN, TA BENNETT, WP METCALF, RA WELSH, JA ECKER, J MODALI, RV ULLRICH, S ROMANO, JW APPELLA, E TESTA, JR GERWIN, BI HARRIS, CC AF LEHMAN, TA BENNETT, WP METCALF, RA WELSH, JA ECKER, J MODALI, RV ULLRICH, S ROMANO, JW APPELLA, E TESTA, JR GERWIN, BI HARRIS, CC TI P53 MUTATIONS, RAS MUTATIONS, AND P53-HEAT SHOCK 70 PROTEIN COMPLEXES IN HUMAN LUNG-CARCINOMA CELL-LINES SO CANCER RESEARCH LA English DT Article ID BRONCHIAL EPITHELIAL-CELLS; TUMOR SUPPRESSOR GENE; WILD-TYPE P53; HEAT-SHOCK; MUTANT P53; T-ANTIGEN; SV40-TRANSFORMED CELLS; TRANSFORMED-CELLS; POINT MUTATIONS; BREAST-CANCER AB The p53 tumor suppressor gene is frequently mutated and the K-ras oncogene is occasionally mutated in primary specimens of human lung carcinomas. These mutated genes also cooperate in the immortalization and neoplastic transformation of rodent cells. To determine whether these mutations are necessary for maintenance of the immortalized and/or neoplastically transformed states of human bronchial epithelial cells, the p53 gene and regions of the ras (K-, H-, and N-) genes were sequenced in nine human lung carcinoma cell lines. Detection of p53 mutations by polymerase chain amplification and direct DNA sequencing was corroborated by p53 immunocytochemistry and coimmunoprecipitation of p53 with heat shock protein 70. p53 and ras genes were frequently, but not always, mutated in the carcinoma cell lines. These data are consistent with the hypothesis that multiple genetic changes involving both protooncogenes and tumor suppressor genes occur during lung carcinogenesis. C1 NCI,HUMAN CARCINOGENESIS LAB,BLDG 37,ROOM 2C05,BETHESDA,MD 20892. NCI,CELL BIOL LAB,BETHESDA,MD 20892. FOX CHASE CANC INST,PHILADELPHIA,PA 19111. NR 72 TC 380 Z9 381 U1 0 U2 3 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD AUG 1 PY 1991 VL 51 IS 15 BP 4090 EP 4096 PG 7 WC Oncology SC Oncology GA FY287 UT WOS:A1991FY28700040 PM 1855224 ER PT J AU HOLLSTEIN, MC PERI, L MANDARD, AM WELSH, JA MONTESANO, R METCALF, RA BAK, M HARRIS, CC AF HOLLSTEIN, MC PERI, L MANDARD, AM WELSH, JA MONTESANO, R METCALF, RA BAK, M HARRIS, CC TI GENETIC-ANALYSIS OF HUMAN ESOPHAGEAL TUMORS FROM 2 HIGH-INCIDENCE GEOGRAPHIC AREAS - FREQUENT P53 BASE SUBSTITUTIONS AND ABSENCE OF RAS MUTATIONS SO CANCER RESEARCH LA English DT Article ID CULTURED HUMAN BRONCHI; HIGH-RISK; NO EVIDENCE; K-RAS; CANCER; DNA; SPECIFICITY; TOBACCO; METABOLISM; ONCOGENES AB Esophageal squamous cell carcinoma (ESC) samples from patients residing in Uruguay and in Normandy, France, where alcoholic beverages and tobacco smoke are major risk factors, were analyzed for point mutations in the p53 tumor suppressor gene. Among 34 tumors (15 from Normandy and 19 from Uruguay) 15 point mutations in the p53 gene that result in amino acid substitutions or chain termination were identified by polymerase chain reaction amplification of exons 5-8 and direct DNA sequencing. Base substitutions in ESC from these high-incidence areas are dispersed over the midregion of the p53 gene. There are differences between ESC and other types of gastrointestinal cancer in the nature of frequent base substitutions. CpG to TpG transitions were far less prevalent in these ESC than in colorectal tumors, whereas G to T transversions, rarely found in colon cancers, were found in one-fourth of the ESC samples. Base substitutions at A:T pairs constitute an important fraction of ESC p53 mutations, in contrast to mutation patterns in most other types of solid tumors. In contrast to the frequent mutation of the p53 gene in these samples, no mutations in the H-, K-, or N-ras genes were found in 16 tumors from Uruguay by direct sequencing of exons in which transforming mutations are known to occur. A previous study on ras mutations in ESC from France was also negative (M. C. Hollstein et al., Cancer Res., 48: 5119-5123, 1988). The role of distinct etiological factors in generating these differences and the potential for linking patient exposure histories with patterns of p53 mutations in high risk populations are considered. C1 NCI,HUMAN CARCINOGENESIS LAB,BLDG 37,ROOM 2C05,BETHESDA,MD 20892. HOSP CLIN DR MANUEL QUINTELA,CEIED,CTR DIGEST DIS,MONTEVIDEO,URUGUAY. CTR BACLESSE,F-14021 CAEN,FRANCE. INT AGCY RES CANC,F-69372 LYONS,FRANCE. NR 56 TC 191 Z9 193 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD AUG 1 PY 1991 VL 51 IS 15 BP 4102 EP 4106 PG 5 WC Oncology SC Oncology GA FY287 UT WOS:A1991FY28700042 PM 1855226 ER PT J AU VAHAKANGAS, K TRIVERS, GE PLUMMER, S HAYES, RB KROKAN, H ROWE, M SWARTZ, RP YEAGER, H HARRIS, CC AF VAHAKANGAS, K TRIVERS, GE PLUMMER, S HAYES, RB KROKAN, H ROWE, M SWARTZ, RP YEAGER, H HARRIS, CC TI 0-6-METHYLGUANINE-DNA METHYLTRANSFERASE AND URACIL DNA GLYCOSYLASE IN HUMAN BRONCHOALVEOLAR LAVAGE CELLS AND PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM TOBACCO SMOKERS AND NONSMOKERS SO CARCINOGENESIS LA English DT Article ID HUMAN GASTRIC-MUCOSA; O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ACTIVITY; HUMAN-FETAL TISSUES; O-6-METHYLGUANINE-DNA METHYLTRANSFERASE; HELA-CELLS; INTERINDIVIDUAL VARIATION; CIGARETTE-SMOKING; ESCHERICHIA-COLI; DRUG-METABOLISM; REPAIR AB Because interindividual variations in the activities of DNA repair enzymes may be a risk factor in the pathogenesis of lung diseases, O6-methylguanine-DNA methyltransferase (O6-MT) and uracil DNA glycosylase (UDG) were measured in broncho-alveolar lavage cell (BALC) and peripheral blood mononuclear cell (PBM) samples from 57 healthy volunteers (25 smokers and 32 non-smokers). According to cotinine determination in 39 cases where serum for this was available, 38% of the self-acclaimed non-smokers had > 10 ng/ml of cotinine in their serum. Whether grouped into smokers and non-smokers according to clinical history or by serum cotinine, there were no statistically significant differences between these groups in O6-NIT or UDG in either of the cell types. However, a tendency towards lower values in smokers was seen. The highest intraindividual variation in O6-MT activity was 7-fold, while the highest interindividual variation reached 18-fold. For UDG, the respective values were 24- and 307-fold. Although the distribution of O6-MT in BALC was different from that in PBM, the data are consistent with unimodality iii both of the cell types. These findings suggest that exposure to cigarette smoke is not entirely responsible for the wide interindividual variation in O6-MT and UDG DNA repair activities. C1 UNIV OULU,DEPT PHARMACOL & TOXICOL,SF-90220 OULU,FINLAND. GEORGETOWN UNIV,MED CTR,DIV PULM DIS & CRIT CARE,WASHINGTON,DC 20007. RP VAHAKANGAS, K (reprint author), NIH,DIV CANC ETIOL,ENVIRONM EPIDEMIOL BRANCH,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. NR 58 TC 39 Z9 39 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD AUG PY 1991 VL 12 IS 8 BP 1389 EP 1394 DI 10.1093/carcin/12.8.1389 PG 6 WC Oncology SC Oncology GA GA911 UT WOS:A1991GA91100005 PM 1860159 ER PT J AU WESTON, A BOWMAN, ED AF WESTON, A BOWMAN, ED TI FLUORESCENCE DETECTION OF BENZO[A]PYRENE - DNA ADDUCTS IN HUMAN LUNG SO CARCINOGENESIS LA English DT Article ID DIOL-EPOXIDE-DNA; COKE-OVEN WORKERS; HUMAN-PLACENTA; HYDROCARBON METABOLITES; MONOCLONAL-ANTIBODIES; HEMOGLOBIN ADDUCTS; SMOKING; EPIDEMIOLOGY; COTININE; TISSUES AB Improved techniques are described for the specific identification of benzo[a]pyrene-diolepoxide (BPDE) - DNA adducts in human tissues. Immunoaffinity chromatograph, synchronous fluorescence spectroscopy and second-derivative synchronous fluorescence spectroscopy have previously been used to detect BPDE - DNA adducts in human placenta. Here we report how these methods, together with HPLC and the generation of complete fluorescence excitation-emission matrices, have been used to identify unequivocally BPDE-DNA adducts in samples of human lung. BPDE nucleotide adducts were isolated with immunoaffinity chromatography columns bearing antibodies raised against the (+/-)anti-7,8-diol-9,10-epoxide-deoxyguanosine adduct of benzo[a]pyrene. These adducts were hyrdrolyzed to tetrahydrotetrols and the hydrolysis products subjected to HPLC. The major product isolated by HPLC, benzo[a]pyrene-7,10/8,9-tetrahydrotetrol, was determined by fluorescence spectroscopy. Using this method levels of BPDE-DNA addicts in the range of 1-40 in 10(8) nucleotides were measured in 6 out of 25 samples, with a lower detection limit of one adduct in 10(8) nucleotides. The data may also indicate that adduct levels show regional variation in different parts of the same lung. RP WESTON, A (reprint author), NCI,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. NR 42 TC 73 Z9 74 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD AUG PY 1991 VL 12 IS 8 BP 1445 EP 1449 DI 10.1093/carcin/12.8.1445 PG 5 WC Oncology SC Oncology GA GA911 UT WOS:A1991GA91100013 PM 1860165 ER PT J AU WILSON, VL FOILES, PG CHUNG, FL POVEY, AC FRANK, AA HARRIS, CC AF WILSON, VL FOILES, PG CHUNG, FL POVEY, AC FRANK, AA HARRIS, CC TI DETECTION OF ACROLEIN AND CROTONALDEHYDE DNA ADDUCTS IN CULTURED HUMAN-CELLS AND CANINE PERIPHERAL-BLOOD LYMPHOCYTES BY P-32 POSTLABELING AND NUCLEOTIDE CHROMATOGRAPHY SO CARCINOGENESIS LA English DT Article ID BRONCHIAL EPITHELIAL-CELLS; DEOXYGUANOSINE ADDUCTS; N-NITROSOPYRROLIDINE; CYTO-TOXICITY; INDUCTION; RATS; CYCLOPHOSPHAMIDE; IDENTIFICATION; FIBROBLASTS; IMMUNOASSAY AB People are constantly being exposed to toxic and carcinogenic aldehydes. However, little is actually known about the mechanisms underlying the toxic and carcinogenic effects of these aldehydes on human cells. The DNA alkylating activities of two of the more toxic and environmentally prominent alpha,beta-unsaturated aldehydes, acrolein and crotonaldehyde, have been studied utilizing P-32-postlabeling and nucleotide chromatographic techniques. Several putative adducts were observed in DNAs isolated from acrolein- and crotonaldehyde-treated human fibroblasts. One of these acrolein-DNA adducts was tentatively identified as the cyclic 1,N2-hydroxypropanodeoxyguanosine product, 3,-(2'-deoxyribosyl)5, 6,7,8-tetrahydro-8-hydroxypyrimido[1,2-a]purine-10-one, by co-chromatography with a chemical standard. The 1,N2-hydroxypropanodeoxyguanosine along with other possible adducts, was also found in DNA isolated from peripheral blood lymphocytes obtained from a dog 1 h after receiving a therapeutic dose of 6.6 mg/kg of cyclophosphamide. These results not only demonstrate the presence of acrolein and crotonaldehyde DNA adducts in treated human cells, but also suggest that these sensitive techniques may be useful to the study of the importance of acrolein to both the carcinogenic and antineoplastic activities of cyclophosphamide and other oxazaphosphorine mustards. C1 CHILDRENS HOSP,KEMPE RES CTR,DENVER,CO 80218. UNIV COLORADO,HLTH SCI CTR,DEPT PATHOL,DENVER,CO 80218. AMER HLTH FDN,NAYLOR DANA INST DIS PREVENT,DIV CHEM CARCINOGENESIS,VALHALLA,NY 10595. PATTERSON INST CANC RES,DEPT CARCINOGENESIS,MANCHESTER M20 9BX,ENGLAND. OREGON STATE UNIV,SCH VET MED,CORVALLIS,OR 97331. NCI,DIV CANC ETIOL,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892. RP WILSON, VL (reprint author), CHILDRENS HOSP,DEPT PATHOL,MOLEC GENET ONCOL LAB,BIZO,1056 E 19TH AVE,DENVER,CO 80218, USA. FU NCI NIH HHS [CA43159] NR 37 TC 54 Z9 56 U1 0 U2 5 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD AUG PY 1991 VL 12 IS 8 BP 1483 EP 1490 DI 10.1093/carcin/12.8.1483 PG 8 WC Oncology SC Oncology GA GA911 UT WOS:A1991GA91100019 PM 1860170 ER PT J AU STEENKISTE, AR BAIM, DS SIPPERLY, ME DESVIGNENICKENS, P ROBERTSON, T DETRE, K AF STEENKISTE, AR BAIM, DS SIPPERLY, ME DESVIGNENICKENS, P ROBERTSON, T DETRE, K TI THE NACI REGISTRY - AN INSTRUMENT FOR THE EVALUATION OF NEW APPROACHES TO CORONARY INTERVENTION SO CATHETERIZATION AND CARDIOVASCULAR DIAGNOSIS LA English DT Article DE ATHERECTOMY; STENT; LASER; PTCA AB The New Approaches to Coronary Intervention (NACI) Registry was developed to collect in-depth data about patients whose coronary artery lesions are being treated with new interventional techniques such as atherectomy, stents, and laser devices. The NACI Registry database distinguishes among several possible "modes" for device use, such as preparatory, planned definitive, and bailout use. Common definitions are used for data collection across all devices, and device-specific forms are used to record procedural details. NACI's unique modular form design facilitates thorough data collection, even for the most complex treatment scenarios. The database structure allows for data analysis at the patient, procedure, lesion, and device levels, as required to perform in-depth analyses of the immediate and long-term success of new devices. Once adequate knowledge of basic device performance has been collected, the Registry structure can also allow expeditious planning and performance of randomized trials comparing a new device to conventional PTCA. C1 HARVARD UNIV,SCH MED,BOSTON,MA 02115. BETH ISRAEL HOSP,DIV CARDIOVASC,BOSTON,MA 02215. NHLBI,CARDIAC DIS BRANCH,BETHESDA,MD 20892. RP STEENKISTE, AR (reprint author), UNIV PITTSBURGH,DEPT EPIDEMIOL,127 PARRAN HALL,PITTSBURGH,PA 15261, USA. NR 2 TC 23 Z9 23 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0098-6569 J9 CATHETER CARDIO DIAG JI Catheter. Cardiovasc. Diagn. PD AUG PY 1991 VL 23 IS 4 BP 270 EP 281 DI 10.1002/ccd.1810230409 PG 12 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA FX143 UT WOS:A1991FX14300008 PM 1889081 ER PT J AU ISSAQ, HJ ATAMNA, IZ MUSCHIK, GM JANINI, GM AF ISSAQ, HJ ATAMNA, IZ MUSCHIK, GM JANINI, GM TI THE EFFECT OF ELECTRIC-FIELD STRENGTH, BUFFER TYPE AND CONCENTRATION ON SEPARATION PARAMETERS IN CAPILLARY ZONE ELECTROPHORESIS SO CHROMATOGRAPHIA LA English DT Article DE CAPILLARY ZONE ELECTROPHORESIS; OPTIMIZATION OF RESOLUTION; APPLIED VOLTAGE; BUFFER CONCENTRATION; BUFFER TYPE; ELECTROOSMOTIC MOBILITY; ELECTROPHORETIC MOBILITY ID MICELLAR SOLUTIONS; INFLUENCE MOBILITY; RESOLUTION; SILICA; CHROMATOGRAPHY; SELECTIVITY; ADSORPTION; PROTEINS AB Probe solutes were used to investigate the effect of buffer type, concentration and applied voltage on solute mobility, column efficiency and resolution in capillary zone electrophoresis. With low conductivity buffers higher concentrations and/or higher voltages could be used to improve column efficiency and resolution. Doubling the concentration of the buffer doubles the amount of heat generated inside the column while doubling the applied voltage cause a 4-fold increase. Solute migration time is approximately an inverse function of the charge density of the buffer's cation. Analysis time is increased by about 30% if the buffer concentration is doubled while it is cut in half if the applied voltage is doubled. Column efficiency is improved (higher theoretical plate count) with increasing buffer concentration and/or applied voltage as long as the heat generated is efficiently dissipated. The separation factor is directly related to analysis time and, therefore, selectivity improves with increasing buffer concentration but decreases with increasing applied voltage. Hence, resolution is optimized by increasing buffer concentration at a moderate applied voltage. C1 PRI DYNCORP,NCI,FREDERICK CANC RES & DEV CTR,POB B,FREDERICK,MD 21702. NR 29 TC 142 Z9 148 U1 0 U2 13 PU FRIEDR VIEWEG SOHN VERLAG GMBH PI WIESBADEN 1 PA PO BOX 5829, W-6200 WIESBADEN 1, GERMANY SN 0009-5893 J9 CHROMATOGRAPHIA JI Chromatographia PD AUG PY 1991 VL 32 IS 3-4 BP 155 EP 161 DI 10.1007/BF02325019 PG 7 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA GE967 UT WOS:A1991GE96700009 ER PT J AU EPSTEIN, SE SIEGALL, CB BIRO, S FU, YM FITZGERALD, D PASTAN, I AF EPSTEIN, SE SIEGALL, CB BIRO, S FU, YM FITZGERALD, D PASTAN, I TI CYTOTOXIC EFFECTS OF A RECOMBINANT CHIMERIC TOXIN ON RAPIDLY PROLIFERATING VASCULAR SMOOTH-MUSCLE CELLS SO CIRCULATION LA English DT Article DE PERCUTANEOUS TRANSLUMINAL CORONARY ANGIOPLASTY; CORONARY RESTENOSIS; TRANSFORMING GROWTH FACTOR-ALPHA; EPIDERMAL GROWTH FACTOR; PSEUDOMONAS EXOTOXIN ID EPIDERMAL GROWTH-FACTOR; LUMINAL CORONARY ANGIOPLASTY; FACTOR RECEPTOR GENE; FACTOR-LIKE MOLECULES; PSEUDOMONAS EXOTOXIN; FUSION PROTEIN; ENDOTHELIAL REGENERATION; ESCHERICHIA-COLI; ARTERIAL INJURY; PTCA REGISTRY AB Background. Restenosis after percutaneous transluminal coronary angioplasty is associated with activation of medial smooth muscle cells (SMCs); they proliferate, migrate to the subintima, and narrow the vessel lumen. Cancer cells often express more cell surface receptors than do normal cells. This has allowed tumor cells to be specifically targeted using cytotoxic agents. We have examined whether a similar concept can be applied to rapidly proliferating but nontransformed SMCs. Pseudomonas exotoxin (PE; MW, 66 kDa) is a potent toxin that kills cells by inhibiting protein synthesis; its toxicity is diminished when its cell recognition domain is deleted to produce a 40-kDa protein (PE40). Methods and Results. A complementary DNA encoding transforming growth factor alpha-(TGF-alpha) was ligated to that encoding PE40 and the chimeric toxin TGF-alpha-PE40, which is cytotoxic to cancer cells displaying epidermal growth factor (EGF) receptors, was expressed in Escherichia coli. The ability of this toxin to kill proliferating SMCs was tested. When cells were seeded at low density (2,500 cells/cm2) and grown in medium supplemented with 10% fetal bovine serum, they were found to be rapidly proliferating; these cells were very sensitive to the cytotoxic effects of TGF-alpha-PE40 (ID50, 4.0 +/- 0.17 ng/ml). In contrast, cytotoxicity was 30-fold less (ID50, 125 +/- 23 ng/ml; p < 0.0004) when cells were in a quiescent state (grown in medium supplemented with 0.5% fetal bovine serum). Conclusions. Competition studies using excess EGF indicated that the cytotoxic effects of TGF-alpha-PE40 are specifically mediated by the EGF receptor. EGF receptor binding analysis demonstrated that rapidly proliferating SMCs display 10-fold more EGF receptors than do quiescent SMCs in vitro. Thus, a chimeric toxin targeted toward the EGF receptor can selectively kill rapidly proliferating SMCs. Whether this toxin or other chimeric toxins directed against other cell surface receptors will effectively inhibit SMCs proliferating in vivo or be useful in preventing restenosis remains to be determined. C1 NCI,DIV CANC BIOL DIAG & CTR,MOLEC BIOL LAB,BETHESDA,MD 20892. RP EPSTEIN, SE (reprint author), NHLBI,CARDIOL BRANCH,BLDG 10,ROOM 7B15,BETHESDA,MD 20892, USA. NR 60 TC 66 Z9 69 U1 0 U2 6 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD AUG PY 1991 VL 84 IS 2 BP 778 EP 787 PG 10 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA FZ374 UT WOS:A1991FZ37400034 PM 1860221 ER PT J AU FURBERG, CD YUSUF, S AF FURBERG, CD YUSUF, S TI ANTIARRHYTHMICS AND VPD SUPPRESSION SO CIRCULATION LA English DT Article DE EDITORIAL COMMENTS; ANTIARRHYTHMICS; CLINICAL TRIALS ID MYOCARDIAL-INFARCTION; DRUG-THERAPY C1 NHLBI, CLIN TRIALS BRANCH, BETHESDA, MD 20892 USA. RP FURBERG, CD (reprint author), WAKE FOREST UNIV, BOWMAN GRAY SCH MED, DEPT PUBL HLTH SCI, 300 S HAWTHORNE RD, WINSTON SALEM, NC 27103 USA. NR 5 TC 6 Z9 6 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0009-7322 EI 1524-4539 J9 CIRCULATION JI Circulation PD AUG PY 1991 VL 84 IS 2 BP 928 EP 930 PG 3 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA FZ374 UT WOS:A1991FZ37400052 PM 1860236 ER PT J AU SIMONS, M WANG, M MCBRIDE, OW KAWAMOTO, S YAMAKAWA, K GDULA, D ADELSTEIN, RS WEIR, L AF SIMONS, M WANG, M MCBRIDE, OW KAWAMOTO, S YAMAKAWA, K GDULA, D ADELSTEIN, RS WEIR, L TI HUMAN NONMUSCLE MYOSIN HEAVY-CHAINS ARE ENCODED BY 2 GENES LOCATED ON DIFFERENT CHROMOSOMES SO CIRCULATION RESEARCH LA English DT Article DE GENE LOCALIZATION; CDNA CLONING; GENE EXPRESSION ID SMOOTH-MUSCLE CELLS; INSITU HYBRIDIZATION; MESSENGER-RNA; DNA-SEQUENCES; LOCALIZATION; EXPRESSION; ATHEROSCLEROSIS; IDENTIFICATION; PROLIFERATION; DIVERSITY AB We report the cloning of cDNAs encoding two different human nonmuscle myosin heavy chains designated NMMHC-A and NMMHC-B. The mRNAs encoding NMMHC-A and NMMHC-B are both 7.5 kb in size but are shown to be the products of different genes, which are localized to chromosome 22q11.2 and chromosome 17p13, respectively. In agreement with previously reported results using avian tissues, we show that the mRNAs encoding the two myosin heavy chain isoforms are differentially expressed in rat nonmuscle and muscle tissues as well as in a number of human cell lines. The cDNA sequence encoding the 5' portion of the NMMHC-A isoform completes the previously published 3' cDNA sequence encoding a human myosin heavy chain, thus providing the cDNA sequence encoding the entire NMMHC-A amino acid sequence. Comparison of this sequence to cDNA clones encoding the amino-terminal one third of the NMMHC-B sequence (amino acids 58-718) shows them to be 89% identical at the amino acid level and 74% identical at the nucleotide level. C1 NHLBI,MOLEC CARDIOL LAB,BLDG 10,ROOM 8N-202,BETHESDA,MD 20892. NCI,BIOCHEM LAB,BETHESDA,MD 20892. ST ELIZABETH HOSP,DEPT CARDIOL,BOSTON,MA. RI Simons, Michael/G-8553-2014 OI Simons, Michael/0000-0003-0348-7734 NR 49 TC 207 Z9 211 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7330 J9 CIRC RES JI Circ.Res. PD AUG PY 1991 VL 69 IS 2 BP 530 EP 539 PG 10 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA FZ293 UT WOS:A1991FZ29300028 PM 1860190 ER PT J AU CAPOGROSSI, MC KACHADORIAN, WA GAMBASSI, G SPURGEON, HA LAKATTA, EG AF CAPOGROSSI, MC KACHADORIAN, WA GAMBASSI, G SPURGEON, HA LAKATTA, EG TI CA-2+ DEPENDENCE OF ALPHA-ADRENERGIC EFFECTS ON THE CONTRACTILE PROPERTIES AND CA-2+ HOMEOSTASIS OF CARDIAC MYOCYTES SO CIRCULATION RESEARCH LA English DT Article DE ALPHA-ADRENOCEPTORS; CARDIAC INOTROPISM; CARDIAC MYOCYTES; CALCIUM; PROTEIN KINASE-C ID PROTEIN-KINASE-C; MEDIATED PHOSPHOINOSITIDE BREAKDOWN; PROMOTING PHORBOL ESTERS; RAT VENTRICULAR MYOCYTES; SARCOPLASMIC-RETICULUM; PURKINJE-FIBERS; CALCIUM RELEASE; BETA-ADRENOCEPTORS; INOTROPIC RESPONSE; PAPILLARY-MUSCLES AB alpha-Adrenergic stimulation is known to enhance myocardial contractility. Adult rat left ventricular myocytes bathed in 1 mM [Ca2+] (Ca0) and electrically stimulated at 0.2 Hz responded to alpha-adrenergic stimulation with 50-mu-M phenylephrine and 1-mu-M propranolol with an increase in twitch amplitude to 177.1 +/- 25.6% of control (mean +/- SEM). In contrast, when cell Ca2+ loading was increased by bathing cells in 5 mM Ca0, alpha-adrenergic stimulation decreased twitch amplitude to 68.6 +/- 8.2% of control. Time-averaged cytosolic [Ca2+] of cells in 1.0 mM Ca0 is enhanced via an increase in the frequency of electrical stimulation. When myocytes were stimulated at 2 Hz in 1 mM Ca0, alpha-adrenergic stimulation did not increase twitch amplitude (103.8 +/- 12.4% of control). In myocytes loaded with the Ca2+ probe indo-1, alpha-adrenergic effects during stimulation at 0.2 Hz (an increase in twitch amplitude in 1 mM Ca0 and a decrease in twitch amplitude in 5 mM Ca0) were associated with similar changes in the indo-1 transient. In 5 mM Ca0, spontaneous Ca2+ releases from the sarcoplasmic reticulum (SR) occurred in the diastolic interval between twitches (2.9 +/- 1.4 spontaneous SR Ca2+ oscillations/min; n = 7); alpha-adrenergic stimulation abolished these oscillations in six of seven cells. Thus, an increase in the frequency of spontaneous diastolic SR Ca2+ release (i.e., Ca2+ overload) is not the mechanism for the negative inotropic effect of alpha-adrenergic stimulation in 5 mM Ca0. In experiments with unstimulated myocytes, we determined whether the effect of alpha-adrenergic stimulation on cell Ca2+ homeostasis and oscillatory SR Ca2+ release observed in 5 mM Ca0 occurs only during electrical stimulation, when voltage-dependent currents are operative, or also at rest. Unstimulated rat ventricular myocytes in 5 mM Ca0 exhibit oscillatory SR Ca2+ release; alpha-adrenergic stimulation decreased the frequency of these oscillations to 53.9 +/- 8.9% of control, and this effect was blocked by 1-mu-M prazosin. In unstimulated indo-1-loaded myocytes alpha-adrenergic stimulation decreased the resting indo-1 fluorescence ratio in 5 mM Ca0, whereas it had no effect in 1 mM Ca0. Additional experiments were aimed at defining a role for Ca2+-activated, phospholipid-dependent protein kinase C (PKC) for the negative inotropic effect of alpha-adrenergic stimulation in 5 mM Ca0. Short-term preexposure to 0.1-mu-M 4-beta-phorbol 12-myristate 13-acetate (PMA) has been shown to maximally activate PKC. PMA, in 5 mM Ca0, decreased contraction and indo-1 transient amplitudes and prevented any additional negative inotropic action by alpha-adrenergic stimulation. Additionally, staurosporine (5 nM), a PKC inhibitor, abolished the alpha-adrenergic-stimulated decrease in twitch amplitude in 5 mM Ca0. Thus, an increase in cytosolic [Ca2+] achieved via an increase in Ca0 or by rapid pacing leads to effects of alpha-adrenergic stimulation on the contractile properties and Ca2+ homeostasis of rat ventricular myocytes that are different from those observed in lower Ca0. These results may be explained with a [Ca2+]-dependent enhancement of the activity of PKC, an enzyme that is activated during alpha-adrenergic stimulation of the myocardium. RP CAPOGROSSI, MC (reprint author), NIA,GERONTOL RES CTR,CARDIOVASC SCI LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 62 TC 64 Z9 64 U1 0 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7330 J9 CIRC RES JI Circ.Res. PD AUG PY 1991 VL 69 IS 2 BP 540 EP 550 PG 11 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA FZ293 UT WOS:A1991FZ29300029 PM 1650298 ER PT J AU PURI, RK LELAND, P AF PURI, RK LELAND, P TI INVIVO TREATMENT WITH INTERFERON CAUSES AUGMENTATION OF IL-2 INDUCED LYMPHOKINE-ACTIVATED KILLER-CELLS IN THE ORGANS OF MICE SO CLINICAL AND EXPERIMENTAL IMMUNOLOGY LA English DT Article DE INVIVO THERAPY; LAK CELLS; CYTOTOXIC LYMPHOCYTES ID RECOMBINANT HUMAN INTERLEUKIN-2; ALPHA-INTERFERON; MULTIPLE INJECTIONS; ADVANCED CANCER; ASIALO GM1; LYMPHOCYTES; INDUCTION; COMBINATION; METASTASES; GAMMA AB Interferon-alpha (IFN-alpha) has been shown to synergize with IL-2 in the regression of a variety of established murine tumours and studies are underway to explore this combination in patients with advanced cancers as well. To understand the mechanism of synergy we have studied lymphokine-activated killer (LAK) cell activity in various compartments of mice in response to IFN-alpha and IL-2 administration. The effects of IFN-gamma, TNF-alpha and IL-4 were also examined. C57BL/6 mice were injected intraperitoneally with HBSS, IL-2 alone, IFN-alpha alone or both, two times a day for 7 days. On days 4 and 8, LAK activity was tested in a 4-h chromium release in cells obtained from lungs, spleen, and liver using fresh MCA-102 tumour cells as targets. The cells from control mice failed to lyse the MCA-102 target. IL-2 caused the generation of LAK activity and an increase in total cell yield in all the organs after 3 days of injection. IFN-alpha failed to generate LAK activity but when administered along with IL-2, caused synergistic enhancement of LAK lysis of MCA-102 target cells. Cell yield in this group was lower as compared with the IL-2-treated group. LAK activity tested after 7 days of IL-2 therapy was significantly decreased compared with that observed after 3 days. However, activity remained at as high a level after 7 days of therapy as after 3 days of therapy in animals treated with IFN-alpha and IL-2. FACS analysis revealed that asialo GM-1+ (ASGM-1) and NK1.1+ cells were increased in number in IL-2 and IL-2 plus IFN-alpha-treated spleen; however, the number of these cells was similar in both groups. In the liver, ASGM-1+ cells were higher in the IL-2 plus IFN-alpha group than in the group treated with IL-2 alone. By in vitro depletion utilizing antibody and Rbc' experiments, it was clear that both ASGM-1+ and NK1.1+ cells from the spleen mediated most of the cytotoxicity of MCA-102 targets. Pre-treatment irradiation (5 Gy) of mice completely abrogated the capability of IL-2 or IL-2 plus IFN-alpha to generate LAK activity. IFN-gamma also had a stimulatory effect on IL-2 induction of LAK activity. Tumour necrosis factor-alpha (TNF-alpha) and IL-4 failed to generate LAK activity and, in combination with IL-2, no additional stimulatory effect was observed. These studies indicate that induction of LAK activity may be at least partly responsible in the mediation of synergistic anti-tumour effects of IFN-alpha and IL-2 or IFN-gamma and IL-2. RP PURI, RK (reprint author), US FDA,CTR BIOL EVALUAT & RES,DIV CYTOKINE BIOL,CELLULAR IMMUNOL LAB,NIH BLDG 29A,ROOM 2B-20,BETHESDA,MD 20892, USA. NR 42 TC 8 Z9 8 U1 0 U2 2 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0009-9104 J9 CLIN EXP IMMUNOL JI Clin. Exp. Immunol. PD AUG PY 1991 VL 85 IS 2 BP 317 EP 325 PG 9 WC Immunology SC Immunology GA FY642 UT WOS:A1991FY64200025 PM 1713814 ER PT J AU HOOKS, JJ DETRICK, B EVANS, CH AF HOOKS, JJ DETRICK, B EVANS, CH TI LEUKOREGULIN, A NOVEL CYTOKINE ENHANCES THE ANTI-HERPESVIRUS ACTIONS OF ACYCLOVIR SO CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article ID NATURAL-KILLER CELLS; UP-REGULATION; INFECTIONS; INTERFERON; INVIVO C1 NIAID,DIV AIDS,VACCINE RES & DEV BRANCH,BETHESDA,MD 20892. NCI,BIOL LAB,TUMOR BIOL SECT,BETHESDA,MD 20892. RP HOOKS, JJ (reprint author), NEI,IMMUNOL LAB,IMMUNOL & VIROL SECT,BETHESDA,MD 20892, USA. NR 22 TC 3 Z9 3 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0090-1229 J9 CLIN IMMUNOL IMMUNOP JI Clin. Immunol. Immunopathol. PD AUG PY 1991 VL 60 IS 2 BP 244 EP 253 DI 10.1016/0090-1229(91)90067-K PG 10 WC Immunology; Pathology SC Immunology; Pathology GA FX156 UT WOS:A1991FX15600008 PM 1649027 ER PT J AU KINGSLAND, LC AF KINGSLAND, LC TI FINALISTS PAPERS, 1990 FROM THE STUDENT PAPER COMPETITION IN MEDICAL INFORMATICS AT THE 14TH ANNUAL SYMPOSIUM ON COMPUTER-APPLICATIONS IN MEDICAL-CARE (SCAMC) SO COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE LA English DT Editorial Material RP KINGSLAND, LC (reprint author), NATL LIB MED,BETHESDA,MD 20209, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0169-2607 J9 COMPUT METH PROG BIO JI Comput. Meth. Programs Biomed. PD AUG PY 1991 VL 35 IS 4 BP 233 EP 238 DI 10.1016/0169-2607(91)90001-A PG 6 WC Computer Science, Interdisciplinary Applications; Computer Science, Theory & Methods; Engineering, Biomedical; Medical Informatics SC Computer Science; Engineering; Medical Informatics GA GM154 UT WOS:A1991GM15400001 ER PT J AU WACHOLDER, S LUBIN, JH DOSEMECI, M GAIL, MH AF WACHOLDER, S LUBIN, JH DOSEMECI, M GAIL, MH TI BIAS DESPITE MASKED ASSESSMENT OF CLINICAL OUTCOMES WHEN AN OUTCOME IS DEFINED AS ONE OF SEVERAL COMPONENT EVENTS SO CONTROLLED CLINICAL TRIALS LA English DT Article DE BLINDED STUDY; DIAGNOSIS; MISCLASSIFICATION; STUDY DESIGN AB Hypothetical examples are presented that show that bias can result from misclassification of clinical outcomes defined as one of several events, even though the assessment of each component event was masked. In one example, the effect of the overall misclassification is to make a treatment that reduces the risk appear to increase it; in another example, misclassification causes the overall treatment effect to appear stronger than it actually is. These anomalies are due to the fact that the misclassification of the overall outcome can be differential, even though the misclassification of the individual components is nondifferential. RP WACHOLDER, S (reprint author), NCI,EPIDEMIOL & BIOSTAT PROGRAM,6130 EXECUT BLVD,EPN 403,BETHESDA,MD 20892, USA. NR 3 TC 9 Z9 9 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0197-2456 J9 CONTROL CLIN TRIALS JI Controlled Clin. Trials PD AUG PY 1991 VL 12 IS 4 BP 457 EP 461 DI 10.1016/0197-2456(91)90006-8 PG 5 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA GD417 UT WOS:A1991GD41700001 PM 1657524 ER PT J AU KAWANO, YI SASAMOTO, Y KOTAKE, S THURAU, SR WIGGERT, B GERY, I AF KAWANO, YI SASAMOTO, Y KOTAKE, S THURAU, SR WIGGERT, B GERY, I TI TRIALS OF VACCINATION AGAINST EXPERIMENTAL AUTOIMMUNE UVEORETINITIS WITH A T-CELL RECEPTOR PEPTIDE SO CURRENT EYE RESEARCH LA English DT Article ID RETINOID-BINDING PROTEIN; MYELIN BASIC-PROTEIN; S-ANTIGEN; ENCEPHALOMYELITIS; RAT; LINES; DETERMINANT; ARTHRITIS; ADJUVANT; COMPLEX AB T lymphocytes which mediate several experimental autoimmune diseases, including encephalomyelitis (EAE) and uveoretinitis (EAU) in Lewis rats, preferentially utilize V-beta-8 gene product in their receptor. Vaccination against a V-beta-8.2-derived peptide was reported to inhibit EAE induction and we report here on the effect of vaccination with this peptide on the development of EAU. Experimental rats were pretreated with the V-beta-8.2 peptide, emulsified in adjuvant (CFA), whereas control animals were untreated or injected with saline in CFA. Rats of all groups were immunized 30 or 40 days later with the immunopathogenic antigen, emulsified in Mycobacterium-enriched CFA. Vaccination with CFA emulsions containing either the V-beta-8.2 peptide or saline, remarkably inhibited the development of the tested diseases, as compared to the untreated controls. Vaccination with the V-beta-8.2 peptide had variable effects in this study on disease development: it inhibited the S-antigen-induced EAU more than did treatment with CFA in four out of six experiments, had a similar effect to that of CFA in one experiment and enhanced the disease in another experiment. Conversely, vaccination with the V-beta-8.2 peptide slightly enhanced the EAU induced by the interphotoreceptor retinoid-binding protein (IRBP), or its dominant determinant, in three of four experiments and had no apparent effect in the fourth experiment. In addition, we could not reproduce the reported protective effect of vaccination with the V-beta-8.2 peptide against induction of EAE. Vaccination with the V-beta-8.2 peptide also had no clear effect on the development of humoral or cellular immune responses against S-antigen. The factors which could possibly cause the variability in the vaccination effect are discussed. C1 NEI,RETINA CELL & MOLEC BIOL LAB,BETHESDA,MD 20892. RP KAWANO, YI (reprint author), NEI,IMMUNOL LAB,BLDG 10,ROOM 10N210,BETHESDA,MD 20892, USA. NR 31 TC 22 Z9 22 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0271-3683 J9 CURR EYE RES JI Curr. Eye Res. PD AUG PY 1991 VL 10 IS 8 BP 789 EP 795 DI 10.3109/02713689109013873 PG 7 WC Ophthalmology SC Ophthalmology GA GC485 UT WOS:A1991GC48500011 PM 1914511 ER PT J AU SMITHGILL, SJ AF SMITHGILL, SJ TI PROTEIN PROTEIN INTERACTIONS - STRUCTURAL MOTIFS AND MOLECULAR RECOGNITION SO CURRENT OPINION IN BIOTECHNOLOGY LA English DT Article ID ANTIBODY-ANTIGEN COMPLEX; DNA-BINDING PROTEINS; LEUCINE ZIPPER; 3-DIMENSIONAL STRUCTURE; CHIMERIC ANTIBODY; MYOD; EXPRESSION; MYC; SPECIFICITY; JUN AB The past year has brought new insights into common structural motifs used for protein-protein interactions by DNA-binding proteins. In addition, there have been significant advances in our understanding of antibody-protein complexes. C1 NCI,GENET LAB,BETHESDA,MD 20892. NR 49 TC 11 Z9 11 U1 1 U2 1 PU CURRENT BIOLOGY LTD PI LONDON PA 34-42 CLEVELAND STREET, LONDON, ENGLAND W1P 6LB SN 0958-1669 J9 CURR OPIN BIOTECH JI Curr. Opin. Biotechnol. PD AUG PY 1991 VL 2 IS 4 BP 568 EP 575 DI 10.1016/0958-1669(91)90082-G PG 8 WC Biochemical Research Methods; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA GG947 UT WOS:A1991GG94700010 PM 1367677 ER PT J AU POLINSKY, RJ AF POLINSKY, RJ TI PHARMACOLOGY OF THE AUTONOMIC NERVOUS-SYSTEM IN MAN SO CURRENT OPINION IN NEUROLOGY AND NEUROSURGERY LA English DT Article AB Lesions within autonomic pathways lead to alterations in neurotransmitter metabolism and neuropeptide function. Technological advances facilitate investigation of central and peripheral changes in man. Neuropharmacological strategies characterize abnormalities in various aspects of neuronal function that serve as a foundation for developing rational therapeutics. RP POLINSKY, RJ (reprint author), NIH,BLDG 10,ROOM 5N262,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CURRENT SCIENCE LTD PI LONDON PA 34-42 CLEVELAND STREET, LONDON, ENGLAND W1P 6LB SN 0951-7383 J9 CURR OPIN NEUROL NEU PD AUG PY 1991 VL 4 IS 4 BP 533 EP 538 PG 6 WC Neurosciences SC Neurosciences & Neurology GA FZ754 UT WOS:A1991FZ75400004 ER PT J AU CARRINGTON, JL CHEN, P YANAGISHITA, M REDDI, AH AF CARRINGTON, JL CHEN, P YANAGISHITA, M REDDI, AH TI OSTEOGENIN (BONE MORPHOGENETIC PROTEIN-3) STIMULATES CARTILAGE FORMATION BY CHICK LIMB BUD CELLS-INVITRO SO DEVELOPMENTAL BIOLOGY LA English DT Article ID GROWTH-FACTOR-BETA; CHONDROGENESIS; PROTEOGLYCANS; CULTURES; DIFFERENTIATION; PURIFICATION; EMBRYO; ASSAY C1 NIDR,BONE CELL BIOL SECT,BETHESDA,MD 20892. NIDR,BONE RES BRANCH,BETHESDA,MD 20892. RP CARRINGTON, JL (reprint author), UNIFORMED SERV UNIV HLTH SCI,F EDWARD HEBERT SCH MED,DEPT ANAT & CELL BIOL,4301 JONES BRIDGE RD,BETHESDA,MD 20889, USA. NR 25 TC 92 Z9 94 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0012-1606 J9 DEV BIOL JI Dev. Biol. PD AUG PY 1991 VL 146 IS 2 BP 406 EP 415 DI 10.1016/0012-1606(91)90242-U PG 10 WC Developmental Biology SC Developmental Biology GA FY577 UT WOS:A1991FY57700014 PM 1713866 ER PT J AU DICKER, M LEIGHTON, EA AF DICKER, M LEIGHTON, EA TI TRENDS IN DIAGNOSED DRUG PROBLEMS AMONG NEWBORNS - UNITED-STATES, 1979-1987 SO DRUG AND ALCOHOL DEPENDENCE LA English DT Article DE DRUG INCIDENCE; DRUG RATES; DRUG TRENDS; DRUG BIRTHS; DRUG HOSPITALIZATIONS ID MATERNAL MARIJUANA; ALCOHOL; ABUSE AB Between 1979 and 1987, there was an estimated 361% increase in the number of drug-affected newborns discharged from the 6000 non-federal, short-stay hospitals in the United States. Per 10 000 newborns, the rate increased 339%, mostly occurring after 1983. The estimated number of drug affected newborns in 1987 was about 13 000 (95% confidence interval: 10 000-15 000). Recognizing that underreporting could have occurred, multiplicative correction factors derived from the literature were employed to produce 'adjusted' estimates. While somewhat arbitrary, the number of drug-affected newborns, adjusted for underreporting, was about 38 000 (95% confidence interval: 30 000-45 000). Both adjusted and unadjusted estimates of the numbers of newborns identified as drug-affected by the present study is much smaller than most of the estimates reported in the literature. Possible reasons for this finding are discussed. C1 WASHINGTON CONSULTING GRP,WASHINGTON,DC 20036. RP DICKER, M (reprint author), NIDA,DIV APPL RES,FINANCING & SERV RES BRANCH,ROOM 9A-42,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. NR 36 TC 7 Z9 7 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0376-8716 J9 DRUG ALCOHOL DEPEN JI Drug Alcohol Depend. PD AUG PY 1991 VL 28 IS 2 BP 151 EP 165 DI 10.1016/0376-8716(91)90071-6 PG 15 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA GD245 UT WOS:A1991GD24500005 PM 1935566 ER PT J AU FUHR, P AGOSTINO, R HALLETT, M AF FUHR, P AGOSTINO, R HALLETT, M TI SPINAL MOTOR-NEURON EXCITABILITY DURING THE SILENT PERIOD AFTER CORTICAL STIMULATION SO ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY LA English DT Article DE SILENT PERIOD; MAGNETIC STIMULATION; CORTICAL STIMULATION; H-REFLEX; INHIBITION; SENSORY NEUROPATHY ID RECIPROCAL INHIBITION; MAGNETIC STIMULATION; INTACT MAN; CORTEX STIMULATION; UNIT RESPONSES; WRITERS CRAMP; HAND MUSCLES; HUMAN WRIST; HUMAN-BRAIN; REFLEX AB During tonic voluntary muscle contraction, a period of electromyographic silence follows the motor evoked potential produced by transcranial stimulation of the contralateral motor cortex. We studied the silent period in the wrist flexors of 3 normal volunteers and a deafferented patient during 20% of maximal contraction. To test the excitability of the spinal motor neuron pool during the period of silence, the H-reflex was evoked in the normal subjects at different intervals after cortical stimulation. The amplitude of the H-reflex in the silent period was expressed as a percentage of the amplitude during complete muscle relaxation. The H-reflex was profoundly depressed at the beginning of the silent period (13.5-27% of the control measurement), but showed a clear tendency to recover toward the end of the silent period despite continued absence of muscle activation (71-84% of the control). Moreover, the silent period in the deafferented patient was of longer duration than can be accounted for by segmental mechanisms. These findings imply, at least in the late part of the silent period, that a reduction in the excitability of the spinal motor neuron pool plays only a minor role in determining the phenomenon and that it is probably caused by lack of cortical drive. RP FUHR, P (reprint author), NINCDS,MED NEUROL BRANCH,HUMAN MOTOR CONTROL SECT,BLDG 10,ROOM 5N226,BETHESDA,MD 20892, USA. NR 32 TC 366 Z9 367 U1 0 U2 9 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0013-4694 J9 ELECTROEN CLIN NEURO JI Electroencephalogr. Clin. Neurophysiol. PD AUG PY 1991 VL 81 IS 4 BP 257 EP 262 DI 10.1016/0168-5597(91)90011-L PG 6 WC Engineering, Biomedical; Clinical Neurology SC Engineering; Neurosciences & Neurology GA GE404 UT WOS:A1991GE40400003 PM 1714819 ER PT J AU FLISS, AE MICHEL, FJ CHEN, CL HOFIG, A BAZER, FW CHOU, JY SIMMEN, RCM AF FLISS, AE MICHEL, FJ CHEN, CL HOFIG, A BAZER, FW CHOU, JY SIMMEN, RCM TI REGULATION OF THE UTEROFERRIN GENE PROMOTER IN ENDOMETRIAL CELLS - INTERACTIONS AMONG ESTROGEN, PROGESTERONE, AND PROLACTIN SO ENDOCRINOLOGY LA English DT Article ID RESISTANT ACID-PHOSPHATASE; MOLECULAR-CLONING; GROWTH-HORMONE; UTERINE; PURIFICATION; EXPRESSION; PREGNANCY; RECEPTORS; PROTEIN; ASSAY AB Expression of the gene for the porcine transplacental iron transport protein uteroferrin (UF) is largely restricted to the uterus, where it is differentially regulated by estrogen (E) and progesterone (P). To study the regulatory mechanisms subserving these effects, a 2-kilobase genomic fragment corresponding to -2005 to 48 nucleotides of the UF gene was ligated up-stream to the reporter gene chloramphenicol acetyltransferase (CAT). This construct (UF-CAT) was transiently transfected into rabbit endometrial (HRE-H9), mouse fibroblastic (AKR-2B), and human choriocarcinoma (JEG-3) cells. The basal gene promoter activity of UF-CAT was exhibited in H9 cells, but not in AKR-2B or JEG-3 cells. In contrast, a simian virus-40 early promoter (SV2) was functional in all three cell lines. The H9 cells were used to examine steroid regulation of the UF gene promoter. The CAT expression in H9 cells primed with E and PRL, but not with E or PRL alone, was stimulated by P. In contrast, basal activity of SV2 in these cells was unaffected by hormones, singly or in combination. To examine the basis for the E/PRL-dependent response to P, levels of P and E receptors in H9 cells were quantified. PRL and E plus PRL increased the number of high affinity sites for P, but had little effect on levels of high affinity sites for E in treated vs. untreated H9 cells. In vivo administration of PRL to cyclic gilts had no effect on levels of endometrial UF mRNA and secreted UF protein; however, E- plus PRL-treated gilts had higher (P < 0.05) levels of endometrial UF mRNA and luminal UF than PRL-treated gilts. These results demonstrate in vitro functional activity of the UF gene promoter and associated 5' flanking region and suggest that sequences within this region may mediate tissue-specific and steroid hormone-regulated expression of the UF gene. Moreover, interactions among E, PRL, and P modulate UF gene expression in vivo and in vitro. C1 UNIV FLORIDA,DEPT ANIM SCI,125 ANIM SCI BLDG,459 SHEALY DR,GAINESVILLE,FL 32611. UNIV FLORIDA,DEPT LARGE ANIM CLIN SCI,GAINESVILLE,FL 32611. NICHHD,HUMAN GENET BRANCH,BETHESDA,MD 20892. FU NICHD NIH HHS [R01 HD021961, HD-21961] NR 40 TC 36 Z9 36 U1 2 U2 2 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD AUG PY 1991 VL 129 IS 2 BP 697 EP 704 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY234 UT WOS:A1991FY23400019 PM 1855467 ER PT J AU PLOUZEK, CA CHOU, JY AF PLOUZEK, CA CHOU, JY TI ISOLATION AND CHARACTERIZATION OF A HUMAN AMNION EPITHELIAL-CELL LINE THAT EXPRESSES THE PREGNANCY-SPECIFIC BETA-1-GLYCOPROTEIN GENE SO ENDOCRINOLOGY LA English DT Article ID CARCINOEMBRYONIC ANTIGEN; SODIUM-BUTYRATE; GLYCOPROTEIN GENES; HUMAN TROPHOBLASTS; MOLECULAR-CLONING; MULTIGENE FAMILY; CDNA; SP1; PROTEIN; MEMBER AB Human pregnancy-specific beta-1-glycoprotein (PSG) is a family of closely related glycoproteins of 72K, 64K, 62K, and 54K. Together with the carcinoembryonic antigen, they form new members of the immunoglobulin superfamily. To study the molecular mechanisms that regulate expression of the PSG gene, we established a human amnion cell line, HAA58OD-8C, immortalized with an origin-defective simian virus-40 (SV40) temperature-sensitive A58 mutant virus. HAA58OD-8C cells were temperature sensitive for maintenance of transformation and expressed genes encoding PSG and the alpha- and beta-subunits of hCG. At the permissive temperature (33 C; transformed phenotype), they expressed low levels of PSG, hCG-alpha, and hCG-beta mRNAs and synthesized low levels of a 48K PSG polypeptide. At the nonpermissive temperature (39.5 C), HAA58OD-8C cells exhibited a differentiated phenotype, expressed increased levels of PSG, hCG-alpha, and hCG-beta mRNAs, and produced high levels of PSG polypeptides of 72K and 48K. Sodium butyrate induced PSG mRNA expression, and in the presence of butyrate, HAA58OD-8C cells produced high amounts of PSG polypeptides of 72K, 62K, and 48K. Ribonuclease protection analysis indicated that similar PSG transcripts were expressed by HAA58OD-8C cells and human term placenta. However, these amnion cells expressed selectively a certain population of PSG transcripts. Our results show that this amnion cell line provides a suitable model for studies of PSG gene expression and regulation. C1 NICHHD,HUMAN GENET BRANCH,BLDG 10,ROOM 95-242,BETHESDA,MD 20892. NR 45 TC 7 Z9 7 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD AUG PY 1991 VL 129 IS 2 BP 950 EP 958 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY234 UT WOS:A1991FY23400054 PM 1855484 ER PT J AU TSUTSUMI, K STROMBERG, C VISWANATHAN, M SAAVEDRA, JM AF TSUTSUMI, K STROMBERG, C VISWANATHAN, M SAAVEDRA, JM TI ANGIOTENSIN-II RECEPTOR SUBTYPES IN FETAL TISSUES OF THE RAT - AUTORADIOGRAPHY, GUANINE-NUCLEOTIDE SENSITIVITY, AND ASSOCIATION WITH PHOSPHOINOSITIDE HYDROLYSIS SO ENDOCRINOLOGY LA English DT Article ID SMOOTH-MUSCLE CELLS; DIVALENT-CATIONS; BINDING; GLAND; STIMULATION; ANTAGONISTS; EXPRESSION; AFFINITY; RENIN; FETUS AB Quantitative autoradiography revealed large numbers of angiotensin-II (AT) receptors in the 18-day-old rat embryo. The selective AT-1 antagonist DuP 753 readily competed for AT receptors in liver, lung parenchyma, and choroid plexus, and these receptors are classified as AT-1 receptors. The selective AT-2 displacers CGP 42112 A and/or PD 123177 competed with high affinity with AT bound to most receptors located in skeletal muscle, skin, diaphragm, bronchi, and stomach, and these receptors are classified as AT-2 receptors. The amount of AT-2 receptors in fetal tissues was more than 10-fold higher than that of AT-1 receptors. In skeletal muscle and skin, DuP 753 competed with AT in the presence of 10(-7) M CGP 42112 A, indicating the presence of small numbers of AT-1 receptors. In liver and lung parenchyma, binding to AT-1 receptors was sensitive to guanine nucleotides. AT binding to AT-2 receptors in fetal skin and skeletal muscle was insensitive to guanine nucleotides. AT stimulated phosphoinositide hydrolysis in liver (ED50, 64 nM) and in skin and skeletal muscle (ED50, 62 nM); this was inhibited by DuP 753 (liver IC50, 38 nM; skin and skeletal muscle IC50, 26 nM), but not by PD 123177 in concentrations up to the micromolar range. AT-1 receptors are probably coupled to G-proteins, and their stimulation increases phosphoinositide hydrolysis. AT-2 receptors may not be linked to G-proteins, their stimulation is not associated with phosphoinositide hydrolysis, and the nature of their second messenger system(s) is presently unknown. RP TSUTSUMI, K (reprint author), NIMH,CLIN SCI LAB,PHARMACOL SECT,9000 ROCKVILLE PIKE,BLDG 10,ROOM 2D-45,BETHESDA,MD 20892, USA. NR 30 TC 142 Z9 143 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD AUG PY 1991 VL 129 IS 2 BP 1075 EP 1082 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY234 UT WOS:A1991FY23400069 PM 1649738 ER PT J AU LAZARWESLEY, E HADCOCK, JR MALBON, CC KUNOS, G ISHAC, EJN AF LAZARWESLEY, E HADCOCK, JR MALBON, CC KUNOS, G ISHAC, EJN TI TISSUE-SPECIFIC REGULATION OF ALPHA-1B, BETA-1, AND BETA-2-ADRENERGIC RECEPTOR MESSENGER-RNAS BY THYROID STATE IN THE RAT SO ENDOCRINOLOGY LA English DT Note ID ADRENERGIC-RECEPTOR; MOLECULAR-CLONING; ALPHA-1-ADRENERGIC RECEPTOR; HORMONE REGULATION; ADENYLATE-CYCLASE; EXPRESSION; ADRENOCEPTORS; GENE; CDNA; NUMBER AB Steady state levels of the mRNAs for alpha-1B, beta-1- and beta-2-adrenergic receptors (alpha-1(B)AR, beta-1AR, beta-2AR) were quantified by DNA excess solution hybridization assays in the heart, lungs, and liver of rats. Tissues for RNA extraction were obtained from euthyroid and thyroidectomized rats and from thyroidectomized rats treated with a single dose of thyroxine. Thyroidectomy resulted in significant decreases in beta-1AR and beta-2AR mRNAs in heart and lung and alpha-1(B)AR mRNA in liver, whereas the levels of beta-2AR mRNA in liver and alpha-1(B)AR mRNA in heart and lung were significantly increased. All these changes were reversed within 20 hours of a single s.c. injection of 1 mg/kg thyroxine. These findings indicate for the first time that thyroid state regulates mRNA levels for adrenergic receptors, and that this regulation is tissue- and receptor-specific. The changes in adrenergic receptor mRNAs correlate with and probably underlie the well documented, thyroid-dependent changes in the cellular densities and physiological reactivities of adrenergic receptors. C1 SUNY STONY BROOK,DEPT PHARMACOL,DIABET & METAB DIS RES PROGRAM,STONY BROOK,NY 11794. NIAAA,PHYSIOL & PHARMACOL STUDIES,BETHESDA,MD 20892. NR 29 TC 50 Z9 50 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD AUG PY 1991 VL 129 IS 2 BP 1116 EP 1118 PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY234 UT WOS:A1991FY23400077 PM 1649740 ER PT J AU SHELBY, MD SOFUNI, T AF SHELBY, MD SOFUNI, T TI TOXICOLOGY TESTING REQUIREMENTS AND THE UNITED-STATES - JAPAN COLLABORATIVE STUDY ON INVITRO TESTS FOR CHROMOSOMAL-ABERRATIONS SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Editorial Material AB As part of the U.S.-Japan Agreement on Cooperation in Research and Development in Science and Technology, Japanese and American scientists met in North Carolina to exchange information on toxicology testing requirements and guidelines in the two countries and to review progress in a collaborative study on detection of chemically induced chromosomal aberations in cultured mammalian cells. C1 NATL INST ENVIRONM HLTH SCI,SETAQAYA KU,TOKYO 158,JAPAN. RP SHELBY, MD (reprint author), NIEHS,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 2 TC 6 Z9 6 U1 0 U2 0 PU NATL INST ENVIRON HEALTH SCI PI RES TRIANGLE PK PA PO BOX 12233, RES TRIANGLE PK, NC 27709 SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD AUG PY 1991 VL 94 BP 255 EP 259 DI 10.2307/3431319 PG 5 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA GG598 UT WOS:A1991GG59800036 PM 1683283 ER PT J AU SCHWETZ, BA MORRISSEY, RE WELSCH, F KAVLOCK, RA AF SCHWETZ, BA MORRISSEY, RE WELSCH, F KAVLOCK, RA TI INVITRO TERATOLOGY SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Editorial Material ID VALIDATION AB The purpose of this conference was to reevaluate the need for and use of in vitro teratology assays; to examine the validation process for in vitro tests; and to discuss progress in the validation of in vitro teratology screens. Participants enthusiastically supported further development of short-term in vivo and in vitro systems both as prescreens for developmental toxicity and as experimental systems to explore mechanisms of action of toxicants. The group strongly endorsed the development of an updated reference list ("gold standard") of known developmental toxicants and nontoxicants as essential to further progress in developing and validating prescreening efforts. Independently, an expert group should further evaluate the performance characteristics for a validated prescreen. The limits of usefulness of prescreens for product development, regulatory use, and mechanistic investigations need to be clearly defined. Finally, too few in vitro teratology prescreens have been evaluated under multiple-laboratory conditions with common, agreed-upon test agents to draw firm conclusions regarding the merit and reproducibility of in vitro teratology prescreens. There was general agreement regarding the need to move several of the assays further along the validation pathway, at least using a short list of reference compounds. C1 CHEM IND INST TOXICOL,RES TRIANGLE PK,NC 27709. US EPA,HERL MD 67,RES TRIANGLE PK,NC 27711. RP SCHWETZ, BA (reprint author), NIEHS,POB 12233 MD D4-02,RES TRIANGLE PK,NC 27709, USA. NR 4 TC 20 Z9 20 U1 0 U2 0 PU NATL INST ENVIRON HEALTH SCI PI RES TRIANGLE PK PA PO BOX 12233, RES TRIANGLE PK, NC 27709 SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD AUG PY 1991 VL 94 BP 265 EP 268 DI 10.2307/3431321 PG 4 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA GG598 UT WOS:A1991GG59800038 PM 1683285 ER PT J AU BEISEL, KW SRINIVASAPPA, J PRABHAKAR, BS AF BEISEL, KW SRINIVASAPPA, J PRABHAKAR, BS TI MOLECULAR-CLONING OF A HEART ANTIGEN THAT CROSS-REACTS WITH A NEUTRALIZING ANTIBODY TO COXSACKIEVIRUS B4 SO EUROPEAN HEART JOURNAL LA English DT Article DE ALPHA CARDIAC MYOSIN HEAVY CHAIN; AUTOANTIGEN; COXSACKIEVIRUS B4; LIGHT MEROMYOSIN; LAMBDA-GT11; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN ID MYOSIN HEAVY-CHAIN; AUTOIMMUNE THYROID-DISEASE; FULL-LENGTH CDNA; ENZYMATIC AMPLIFICATION; MONOCLONAL-ANTIBODY; AUTO-ANTIGEN; RAT ALPHA; SEQUENCES; MYOCARDITIS; PROTEINS C1 UNIV NEBRASKA,MED CTR,DEPT PATHOL & MICROBIOL,OMAHA,NE 68105. NIDR,ARBEITSGRP ORAL MED,BETHESDA,MD 20892. NR 35 TC 9 Z9 11 U1 0 U2 0 PU W B SAUNDERS CO LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0195-668X J9 EUR HEART J JI Eur. Heart J. PD AUG PY 1991 VL 12 SU D BP 60 EP 64 PG 5 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA GB443 UT WOS:A1991GB44300016 PM 1717274 ER PT J AU LANG, T WARBURG, A SACKS, DL CROFT, SL LANE, RP BLACKWELL, JM AF LANG, T WARBURG, A SACKS, DL CROFT, SL LANE, RP BLACKWELL, JM TI TRANSMISSION AND SCANNING EM-IMMUNOGOLD LABELING OF LEISHMANIA-MAJOR LIPOPHOSPHOGLYCAN IN THE SANDFLY PHLEBOTOMUS-PAPATASI SO EUROPEAN JOURNAL OF CELL BIOLOGY LA English DT Article DE IMMUNOGOLD-EM; LEISHMANIA; LIPOPHOSPHOGLYCAN; SANDFLY ID PROMASTIGOTES; EXPRESSION; PSYCHODIDAE; ATTACHMENT; MICROSCOPY; DIPTERA; HOST AB Previous studies using immunostaining and light microscopy demonstrated expression of Leishmania major lipophosphoglycan (LPG) on parasites developing in the sandfly gut from 2 days post infection. By days 4 to 7 post infection, there appeared to be large amounts of parasite-free LPG deposited on/in the microvilli and epithelial cells lining the thoracic midgut, while forward migration of parasites and the morphological changes which accompany metacyclogenesis were associated with developmental modification of the LPG molecules. Studies presented here examine this process with much greater precision using electron microscopy and immunogold labeling techniques to study the different developmental forms (nectomonads, haptomonads, paramastigotes, and metacyclics) of promastigotes in the sandfly gut. Results obtained using LPG-specific monoclonal antibodies (WIC79.3, 45D3 and the metacyclic-specific 3F12) show (1) gold labeling over the cell surface, within the flagellar pocket, and extending along the entire length of the flagellum of electron-dense nectomonads observed in the abdominal and thoracic midgut regions on days 4 and 7 post infection, and of electron-lucid haptomonads in the foregut, (2) dense labeling around the flagellar tips, by which nectomonad forms bind to the midgut microvilli, but not on the microvilli themselves or within the epithelial cells lining the midgut, (3) significant metacyclic-specific (3F12) labeling on nectomonad forms in the lumen of the midgut and attached to the microvilli, and (4) dense labeling on the cell surface of electron-lucid paramastigotes in the esophagus and in the filamentous matrix surrounding paramastigote and metacyclic forms in the esophagus and pharynx. These results are discussed in the light of the proposed roles for LPG in parasite attachment to, and survival in, the sandfly gut. C1 UNIV LONDON LONDON SCH HYG & TROP MED,DEPT MED PARASITOL,KEPPEL ST,LONDON WC1E 7HT,ENGLAND. NIAID,PARASIT DIS LAB,IMMUNOBIOL & CELL BIOL SECT,BETHESDA,MD 20892. RI Blackwell, Jenefer/H-3015-2015 FU Wellcome Trust NR 27 TC 34 Z9 35 U1 1 U2 2 PU WISSENSCHAFTLICHE VERLAG GMBH PI STUTTGART 10 PA BIRKENWALDSTRASSE 44 POSTFACH 105339, W-7000 STUTTGART 10, GERMANY SN 0171-9335 J9 EUR J CELL BIOL JI Eur. J. Cell Biol. PD AUG PY 1991 VL 55 IS 2 BP 362 EP 372 PG 11 WC Cell Biology SC Cell Biology GA GD445 UT WOS:A1991GD44500020 PM 1935998 ER PT J AU HATORI, A SHIGEMATSU, A MCCORMICK, AM WILLHITE, CC SHARMA, RP AF HATORI, A SHIGEMATSU, A MCCORMICK, AM WILLHITE, CC SHARMA, RP TI TEMPORAL DISTRIBUTION OF RETINOIC ACID AND CELLULAR RETINOIC ACID-BINDING PROTEIN (CRABP) IN THE FETAL HAMSTER SO EXPERIMENTAL AND MOLECULAR PATHOLOGY LA English DT Article ID CENTRAL-NERVOUS-SYSTEM; CHICK LIMB BUD; VITAMIN-A; MOUSE EMBRYO; NEURAL-TUBE; EXPRESSION; RAT; IDENTIFICATION; MICE; PURIFICATION C1 UTAH STATE UNIV,TOXICOL PROGRAM,LOGAN,UT 84322. NIA,BETHESDA,MD 20892. INST WHOLE BODY METAB,CHIBA,JAPAN. UNIV CALIF BERKELEY,DEPT HLTH SERV,BERKELEY,CA 94720. NR 37 TC 6 Z9 6 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4800 J9 EXP MOL PATHOL JI Exp. Mol. Pathol. PD AUG PY 1991 VL 55 IS 1 BP 38 EP 54 DI 10.1016/0014-4800(91)90017-R PG 17 WC Pathology SC Pathology GA GD640 UT WOS:A1991GD64000005 PM 1653151 ER PT J AU REED, JC TALWAR, HS CUDDY, M BAFFY, G WILLIAMSON, J RAPP, UR FISHER, GJ AF REED, JC TALWAR, HS CUDDY, M BAFFY, G WILLIAMSON, J RAPP, UR FISHER, GJ TI MITOCHONDRIAL PROTEIN-P26 BCL2 REDUCES GROWTH-FACTOR REQUIREMENTS OF NIH3T3 FIBROBLASTS SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID KINASE-C; FOLLICULAR LYMPHOMA; GENE-EXPRESSION; CA-2+ TRANSPORT; MESSENGER-RNA; CELL-GROWTH; MYC; SURVIVAL; RAS; DIACYLGLYCEROL C1 UNIV PENN,SCH MED,DEPT BIOCHEM,PHILADELPHIA,PA 19104. NCI,RES FACIL FREDERICK,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21710. UNIV MICHIGAN,SCH MED,DEPT DERMATOL,ANN ARBOR,MI 48109. RP REED, JC (reprint author), UNIV PENN,SCH MED,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104, USA. FU NCI NIH HHS [CA49576]; NIAMS NIH HHS [AR39691]; PHS HHS [FO5DW04545] NR 35 TC 30 Z9 30 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD AUG PY 1991 VL 195 IS 2 BP 277 EP 283 DI 10.1016/0014-4827(91)90374-4 PG 7 WC Oncology; Cell Biology SC Oncology; Cell Biology GA FX957 UT WOS:A1991FX95700001 PM 2070813 ER PT J AU GUO, M KIM, LT AKIYAMA, SK GRALNICK, HR YAMADA, KM GRINNELL, F AF GUO, M KIM, LT AKIYAMA, SK GRALNICK, HR YAMADA, KM GRINNELL, F TI ALTERED PROCESSING OF INTEGRIN RECEPTORS DURING KERATINOCYTE ACTIVATION SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID GROWTH FACTOR-BETA; HUMAN FIBRONECTIN RECEPTOR; CELL-SURFACE RECEPTORS; EXTRACELLULAR-MATRIX; MONOCLONAL-ANTIBODIES; HUMAN-SKIN; ADHESION; EXPRESSION; MIGRATION; LOCALIZATION C1 UNIV TEXAS,SW MED CTR,DEPT CELL BIOL & NEUROSCI,DALLAS,TX 75235. NIDR,DEV BIOL LAB,BETHESDA,MD 20892. NIH,CTR CLIN,HEMATOL SERV,BETHESDA,MD 20892. OI Yamada, Kenneth/0000-0003-1512-6805 FU NCI NIH HHS [CA 14609, CA 45515]; NIGMS NIH HHS [GM31321] NR 39 TC 60 Z9 61 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD AUG PY 1991 VL 195 IS 2 BP 315 EP 322 DI 10.1016/0014-4827(91)90379-9 PG 8 WC Oncology; Cell Biology SC Oncology; Cell Biology GA FX957 UT WOS:A1991FX95700006 PM 2070815 ER PT J AU CHEN, P CARRINGTON, JL HAMMONDS, RG REDDI, AH AF CHEN, P CARRINGTON, JL HAMMONDS, RG REDDI, AH TI STIMULATION OF CHONDROGENESIS IN LIMB BUD MESODERM CELLS BY RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2B (BMP-2B) AND MODULATION BY TRANSFORMING GROWTH FACTOR-BETA-1 AND FACTOR-BETA-2 SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID FACTOR-BETA; AFFINITY-CHROMATOGRAPHY; EXTRACELLULAR-MATRIX; CULTURE; CHONDROCYTES; INVITRO C1 NIDR,BONE CELL BIOL SECT,BLDG 30,ROOM 211,BETHESDA,MD 20892. UNIFORMED SERV UNIV HLTH SCI,F EDWARD HEBERT SCH MED,DEPT ANAT & CELL BIOL,BETHESDA,MD 20814. GENENTECH INC,S SAN FRANCISCO,CA 94080. NR 31 TC 175 Z9 177 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD AUG PY 1991 VL 195 IS 2 BP 509 EP 515 DI 10.1016/0014-4827(91)90403-H PG 7 WC Oncology; Cell Biology SC Oncology; Cell Biology GA FX957 UT WOS:A1991FX95700030 PM 2070831 ER PT J AU JAMES, SL AF JAMES, SL TI THE EFFECTOR FUNCTION OF NITROGEN-OXIDES IN HOST DEFENSE AGAINST PARASITES SO EXPERIMENTAL PARASITOLOGY LA English DT Review ID ACTIVATED MACROPHAGES; L-ARGININE; SCHISTOSOMA-MANSONI; NITRIC-OXIDE; MECHANISM; INVITRO; INVIVO RP JAMES, SL (reprint author), NIAID,PARASIT DIS LAB,IMMUNOL & CELL BIOL SECT,BETHESDA,MD 20892, USA. NR 19 TC 28 Z9 28 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4894 J9 EXP PARASITOL JI Exp. Parasitol. PD AUG PY 1991 VL 73 IS 2 BP 223 EP 226 DI 10.1016/0014-4894(91)90025-R PG 4 WC Parasitology SC Parasitology GA GB503 UT WOS:A1991GB50300011 PM 1889475 ER PT J AU PASTAN, I WILLINGHAM, MC GOTTESMAN, M AF PASTAN, I WILLINGHAM, MC GOTTESMAN, M TI MOLECULAR MANIPULATIONS OF THE MULTIDRUG TRANSPORTER - A NEW ROLE FOR TRANSGENIC MICE SO FASEB JOURNAL LA English DT Article DE CHEMOTHERAPY; CANCER; TRANSPORT PROTEIN; DRUG RESISTANCE ID RESISTANCE GENE; P-GLYCOPROTEIN; EXPRESSION; CELLS; MDR1; TISSUES; LOCALIZATION; VINBLASTINE; MUTATIONS; SITES AB Multidrug resistance in human cancer is associated with overexpression of the MDR1 gene which encodes a 170,000 molecular weight membrane glycoprotein that transports cytotoxic drugs out of cancer cells. The MDR1 gene is normally expressed in intestine, kidney, liver, and adrenal glands, and in tumors derived from these tissues, but it is not expressed in normal bone marrow. Transgenic mice that express the MDR1 gene in their bone marrow have been developed, and because of this expression these mice are resistant to the bone marrow-suppressive effects of daunomycin, doxorubicin, taxol, and several other anticancer drugs. These mice can be used in several different ways to develop new types of drugs to treat human cancer. C1 NCI,CELL BIOL LAB,BETHESDA,MD 20892. RP PASTAN, I (reprint author), NCI,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 28 TC 52 Z9 52 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD AUG PY 1991 VL 5 IS 11 BP 2523 EP 2528 PG 6 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA GB094 UT WOS:A1991GB09400004 PM 1868977 ER PT J AU FAIL, PA GEORGE, JD SEELY, JC GRIZZLE, TB HEINDEL, JJ AF FAIL, PA GEORGE, JD SEELY, JC GRIZZLE, TB HEINDEL, JJ TI REPRODUCTIVE TOXICITY OF BORIC-ACID IN SWISS (CD-1) MICE - ASSESSMENT USING THE CONTINUOUS BREEDING PROTOCOL SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Article ID DOSE LEVELS; BORON C1 NIEHS,NATL TOXICOL PROGRAM,DEV & REPROD TOXICOL,RES TRIANGLE PK,NC 27709. PATHCO INC,RES TRIANGLE PK,NC 27709. RP FAIL, PA (reprint author), RES TRIANGLE INST,POB 12194,RES TRIANGLE PK,NC 27709, USA. FU NIEHS NIH HHS [N01-ES-65141] NR 48 TC 43 Z9 44 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD AUG PY 1991 VL 17 IS 2 BP 225 EP 239 DI 10.1016/0272-0590(91)90215-P PG 15 WC Toxicology SC Toxicology GA FZ997 UT WOS:A1991FZ99700002 PM 1765217 ER PT J AU GULATI, DK HOPE, E TEAGUE, J CHAPIN, RE AF GULATI, DK HOPE, E TEAGUE, J CHAPIN, RE TI REPRODUCTIVE TOXICITY ASSESSMENT BY CONTINUOUS BREEDING IN SPRAGUE-DAWLEY RATS - A COMPARISON OF 2 STUDY DESIGNS SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Article C1 NIEHS,NATL TOXICOL PROGRAM,DEV & REPROD TOXICOL GRP,RES TRIANGLE PK,NC 27709. ANALYT SCI INC,DURHAM,NC 27713. RP GULATI, DK (reprint author), ENVIRONM HLTH RES & TESTING INC,2514 REGENCY RD,LEXINGTON,KY 40503, USA. NR 19 TC 10 Z9 10 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD AUG PY 1991 VL 17 IS 2 BP 270 EP 279 DI 10.1016/0272-0590(91)90218-S PG 10 WC Toxicology SC Toxicology GA FZ997 UT WOS:A1991FZ99700005 PM 1765220 ER PT J AU DIETZ, DD ABDO, KM HASEMAN, JK EUSTIS, SL HUFF, JE AF DIETZ, DD ABDO, KM HASEMAN, JK EUSTIS, SL HUFF, JE TI COMPARATIVE TOXICITY AND CARCINOGENICITY STUDIES OF TETRACYCLINE AND OXYTETRACYCLINE IN RATS AND MICE SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Article ID STATISTICAL ISSUES; BILIARY-EXCRETION; LIVER C1 NIEHS,NATL TOXICOL PROGRAM,DIV BIOMETRY & RISK ASSESSMENT,RES TRIANGLE PK,NC 27709. LIGGETT GRP INC,DURHAM,NC 27702. RP DIETZ, DD (reprint author), NIEHS,NATL TOXICOL PROGRAM,DIV TOXICOL RES & TESTING,RES TRIANGLE PK,NC 27709, USA. NR 51 TC 13 Z9 13 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD AUG PY 1991 VL 17 IS 2 BP 335 EP 346 DI 10.1016/0272-0590(91)90223-Q PG 12 WC Toxicology SC Toxicology GA FZ997 UT WOS:A1991FZ99700010 PM 1765221 ER PT J AU DIETZ, DD LEININGER, JR RAUCKMAN, EJ THOMPSON, MB CHAPIN, RE MORRISSEY, RL LEVINE, BS AF DIETZ, DD LEININGER, JR RAUCKMAN, EJ THOMPSON, MB CHAPIN, RE MORRISSEY, RL LEVINE, BS TI TOXICITY STUDIES OF ACETONE ADMINISTERED IN THE DRINKING-WATER OF RODENTS SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Article ID INDUCED TESTICULAR ATROPHY; CHRONIC-ALCOHOLIC MEN; MALE-RAT; STARVED RATS; ETHANOL; METABOLISM; ACID; POTENTIATION; KETONE; LIVER C1 MICROBIOL ASSOCIATES INC,BETHESDA,MD 20816. RP DIETZ, DD (reprint author), NIEHS,NATL TOXICOL PROGRAM,DIV TOXICOL RES & TESTING,RES TRIANGLE PK,NC 27709, USA. OI Chapin, Robert/0000-0002-5997-1261 NR 65 TC 12 Z9 12 U1 0 U2 3 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD AUG PY 1991 VL 17 IS 2 BP 347 EP 360 DI 10.1016/0272-0590(91)90224-R PG 14 WC Toxicology SC Toxicology GA FZ997 UT WOS:A1991FZ99700011 PM 1765222 ER PT J AU MERLINO, GT STAHLE, C JHAPPAN, C LINTON, R MAHON, KA WILLINGHAM, MC AF MERLINO, GT STAHLE, C JHAPPAN, C LINTON, R MAHON, KA WILLINGHAM, MC TI INACTIVATION OF A SPERM MOTILITY GENE BY INSERTION OF AN EPIDERMAL GROWTH-FACTOR RECEPTOR TRANSGENE WHOSE PRODUCT IS OVEREXPRESSED AND COMPARTMENTALIZED DURING SPERMATOGENESIS SO GENES & DEVELOPMENT LA English DT Article DE FLAGELLAR MICROTUBULE; RECESSIVE MUTATION; STERILITY; TESTIS; TRANSGENIC; TRANSLATIONAL CONTROL ID MICE; MOUSE; EXPRESSION; MUTATION; PROMOTER; CELLS; LOCUS; TRANSCRIPTION; SEQUENCE AB Transgenic mice were generated with a human epidermal growth factor (EGF) receptor cDNA driven by the chicken beta-actin gene promoter. One line (AE24) that exhibited a unique expression pattern in which dramatically elevated levels of EGF receptor RNA were found only in the testis was established, suggesting that the beta-actin promoter was being influenced by an adjacent testis-specific enhancer. EGF receptor RNA was detected in primary spermatocytes, whereas the synthesis of receptor protein was restricted to elongate spermatids, indicating that transgene expression was under translational control. At spermiation, the EGF receptor was sequestered in residual bodies and excluded from mature sperm by a compartmentalization mechanism. About half of AE24 homozygous males were sterile because of sperm paralysis, whereas heterozygous males and females of either genotype were completely fertile. Electron microscopic analysis of sperm flagella from sterile AE24 homozygotes revealed an aberrant axonemal structure in which outer doublet microtubules were missing from the middle piece, resembling changes observed in the sperm of some infertile humans. Flagellar axonemal disassembly was observed in the vas deferens and epididymis but not in the testis, suggesting that outer doublets were assembled in a grossly normal manner but possessed a latent instability. These results demonstrate that in the AE24 mouse line the EGF receptor transgene was integrated into and inactivated an endogenous autosomal gene, causing sperm flagellar axonemal disruption and male sterility. C1 NICHHD, MAMMALIAN GENES & DEV LAB, BETHESDA, MD 20892 USA. RP MERLINO, GT (reprint author), NCI, DIV CANC BIOL DIAG & CTR, MOLEC BIOL LAB, BETHESDA, MD 20892 USA. NR 43 TC 38 Z9 40 U1 0 U2 0 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0890-9369 J9 GENE DEV JI Genes Dev. PD AUG PY 1991 VL 5 IS 8 BP 1395 EP 1406 DI 10.1101/gad.5.8.1395 PG 12 WC Cell Biology; Developmental Biology; Genetics & Heredity SC Cell Biology; Developmental Biology; Genetics & Heredity GA GA075 UT WOS:A1991GA07500008 PM 1714416 ER PT J AU SIRACUSA, LD ALVORD, WG BICKMORE, WA JENKINS, NA COPELAND, NG AF SIRACUSA, LD ALVORD, WG BICKMORE, WA JENKINS, NA COPELAND, NG TI INTERSPECIFIC BACKCROSS MICE SHOW SEX-SPECIFIC DIFFERENCES IN ALLELIC INHERITANCE SO GENETICS LA English DT Article ID GENETIC-LINKAGE MAP; CHROMOSOMAL LOCALIZATION; MUS-SPRETUS; MOUSE; LOCUS; MARKERS; CROSSES; STRAIN AB Transmission distortion is identified as a difference in transmission frequency of two alleles from the normal 1:1 Mendelian segregation in diploid organisms. Transmission distortion can extend over part or all of a chromosome. The recent development of interspecific mouse backcrosses has provided a powerful method for multilocus mapping of entire chromosomes in a single cross, and consequently for identifying distortions in allelic inheritance. We used an interspecific backcross of [(C57BL/6J x Mus spretus)F1 x C57BL/6J] mice to map molecular loci to mouse chromosome 2 and had previously found that the distal region of the chromosome showed distortions in allelic inheritance. We now report the mapping of five loci (Actc-1, D2Hgu1, His-1, Hox-4.1 and Neb) to chromosome 2, which, in addition to the Abl, Ada, B2m, Bmp-2a, Hc, Emv-15, Fshb, Hck-1, Pax-1, Pck-1, Spana-2 and Vim loci previously mapped in our interspecific backcross, serve as markers to measure allelic inheritance along approximately 75% of mouse chromosome 2. Statistical analyses are used to identify and delimit chromosomal regions showing transmission distortion and to determine whether there are sex-specific differences in allelic inheritance. These studies provide evidence for sex-specific differences in allelic inheritance for chromosome 2 and suggest biological explanations for this form of transmission distortion. C1 NCI,FREDERICK CANC RES & DEV CTR,DATA MANAGEMENT SERV INC,FREDERICK,MD 21702. WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND. RP SIRACUSA, LD (reprint author), NCI,FREDERICK CANC RES & DEV CTR,MAMMALIAN GENET LAB,ABL BASIC RES PROGRAM,FREDERICK,MD 21702, USA. RI Bickmore, Wendy/C-7314-2013 OI Bickmore, Wendy/0000-0001-6660-7735 FU NCI NIH HHS [N01-CO-74101, N01-CO-74103]; NIGMS NIH HHS [GM12721-01] NR 24 TC 38 Z9 38 U1 0 U2 0 PU GENETICS PI BALTIMORE PA 428 EAST PRESTON ST, BALTIMORE, MD 21202 SN 0016-6731 J9 GENETICS JI Genetics PD AUG PY 1991 VL 128 IS 4 BP 813 EP 821 PG 9 WC Genetics & Heredity SC Genetics & Heredity GA FZ270 UT WOS:A1991FZ27000015 PM 1916246 ER PT J AU MAHER, ER BENTLEY, E YATES, JRW LATIF, F LERMAN, M ZBAR, B AFFARA, NA FERGUSONSMITH, MA AF MAHER, ER BENTLEY, E YATES, JRW LATIF, F LERMAN, M ZBAR, B AFFARA, NA FERGUSONSMITH, MA TI MAPPING OF THE VON HIPPEL-LINDAU DISEASE LOCUS TO A SMALL REGION OF CHROMOSOME-3P BY GENETIC-LINKAGE ANALYSIS SO GENOMICS LA English DT Article ID RENAL-CELL CARCINOMA; SHORT ARM; DELETION; LOCALIZATION C1 NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. RP MAHER, ER (reprint author), UNIV CAMBRIDGE,DEPT PATHOL,CAMBRIDGE,ENGLAND. RI MAHER, EAMONN/A-9507-2008 OI MAHER, EAMONN/0000-0002-6226-6918 NR 30 TC 74 Z9 74 U1 0 U2 10 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD AUG PY 1991 VL 10 IS 4 BP 957 EP 960 DI 10.1016/0888-7543(91)90185-H PG 4 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA FW380 UT WOS:A1991FW38000014 PM 1680799 ER PT J AU KIRKNESS, EF KUSIAK, JW FLEMING, JT MENNINGER, J GOCAYNE, JD WARD, DC VENTER, JC AF KIRKNESS, EF KUSIAK, JW FLEMING, JT MENNINGER, J GOCAYNE, JD WARD, DC VENTER, JC TI ISOLATION, CHARACTERIZATION, AND LOCALIZATION OF HUMAN GENOMIC DNA ENCODING THE BETA-1 SUBUNIT OF THE GABA-A RECEPTOR (GABRB1) SO GENOMICS LA English DT Article ID NICOTINIC ACETYLCHOLINE-RECEPTOR; DEPENDENT PROTEIN-KINASE; SEQUENCE-ANALYSIS; FUNCTIONAL EXPRESSION; A-RECEPTORS; BENZODIAZEPINE RECEPTORS; GENES; BRAIN; HYBRIDIZATION; CLONING C1 NINCDS,MOLEC & CELLULAR NEUROBIOL LAB,RECEPTOR BIOCHEM & MOLEC BIOL SECT,BETHESDA,MD 20892. NIAAA,ADAMHA,PHYSIOL & PHARMACOL STUDIES LAB,MOLEC NEUROBIOL SECT,ROCKVILLE,MD 20852. YALE UNIV,SCH MED,DEPT HUMAN GENET,NEW HAVEN,CT 06510. NR 38 TC 53 Z9 55 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD AUG PY 1991 VL 10 IS 4 BP 985 EP 995 DI 10.1016/0888-7543(91)90189-L PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA FW380 UT WOS:A1991FW38000018 PM 1655634 ER PT J AU MOORE, SK APPELLA, E VILLAR, CJ KOZAK, CA AF MOORE, SK APPELLA, E VILLAR, CJ KOZAK, CA TI MAPPING OF THE MOUSE 86-KDA HEAT-SHOCK PROTEIN EXPRESSED GENE (HSP86-1) ON CHROMOSOME 12 AND RELATED GENES ON CHROMOSOMES 3, 4, 9, AND 11 SO GENOMICS LA English DT Article ID NUCLEOTIDE-SEQUENCE; STRESS PROTEINS; COGNATE PROTEIN; RAT-LIVER; CLONING; MURINE; ASSIGNMENT; RECEPTOR; HSP70; CELLS C1 NIAID,MOLEC BIOL LAB,BETHESDA,MD 20892. RP MOORE, SK (reprint author), NCI,CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 69 TC 20 Z9 21 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD AUG PY 1991 VL 10 IS 4 BP 1019 EP 1029 DI 10.1016/0888-7543(91)90193-I PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA FW380 UT WOS:A1991FW38000022 PM 1916807 ER PT J AU HALLFRISCH, J AF HALLFRISCH, J TI GERIATRIC NUTRITION - A COMPREHENSIVE REVIEW - MORLEY,JE, GLICK,Z, RUBENSTEIN,LZ SO GERONTOLOGIST LA English DT Book Review RP HALLFRISCH, J (reprint author), NIA,GERONTOL RES CTR,METAB SECT,BALTIMORE,MD 21224, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 0016-9013 J9 GERONTOLOGIST JI Gerontologist PD AUG PY 1991 VL 31 IS 4 BP 565 EP 566 PG 2 WC Gerontology SC Geriatrics & Gerontology GA FZ431 UT WOS:A1991FZ43100023 ER PT J AU PIZZO, P AF PIZZO, P TI PSEUDOMONAS SO HOSPITAL PRACTICE LA English DT Article; Proceedings Paper CT SYMP ON CURRENT CLINICAL MANAGEMENT OF INFECTIONS : USE OF THIRD-GENERATION CEPHALOSPORINS CY JAN 21, 1991 CL PARK CITY, UT RP PIZZO, P (reprint author), NCI,INFECT DIS SECT,BETHESDA,MD 20892, USA. NR 0 TC 2 Z9 2 U1 1 U2 1 PU MCGRAW HILL HEALTHCARE PUBLICATIONS PI MINNEAPOLIS PA 4530 WEST 77TH ST, MINNEAPOLIS, MN 55435-5000 SN 8750-2836 J9 HOSP PRACT JI Hosp. Pract. PD AUG PY 1991 VL 26 SU 4 BP 18 EP 21 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA GA043 UT WOS:A1991GA04300004 PM 1918211 ER PT J AU SACK, GH TALBOT, CC MCCARTHY, BG HARRIS, EL KASTNER, D GRUBERG, L PRAS, M AF SACK, GH TALBOT, CC MCCARTHY, BG HARRIS, EL KASTNER, D GRUBERG, L PRAS, M TI EXCLUSION OF LINKAGE BETWEEN FAMILIAL MEDITERRANEAN FEVER AND THE HUMAN SERUM AMYLOID-A (SAA) GENE-CLUSTER SO HUMAN GENETICS LA English DT Note ID SEQUENCE AB We studied the relationship between the autosomal recessive trait familial Mediterranean fever (FMF) and the serum amyloid A (SAA) genes by comparing alleles of a highly polymorphic dinucleotide repeat and a conventional restriction fragment length polymorphism (RFLP) in the SAA gene cluster in Israeli FMF kindreds. By haplotype analysis, our data indicate a minimum crossover frequency of 22% between the SAA gene marker and FMF. By conventional linkage analysis this eliminates a minimum of 10.4 cM including and surrounding the SAA gene cluster as the site of the FMF mutation although SAA proteins are prominent physiologic markers of the acute attacks. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT MED,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT PEDIAT,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,BALTIMORE,MD 21205. KENNEDY INST,BALTIMORE,MD. SCH HYG & PUBL HLTH,BALTIMORE,MD. NIH,DIV ALLERGY ARTHRIT & INFECT DIS,BETHESDA,MD 20892. TEL HASHOMER HOSP,HELLER INST MED RES,TEL AVIV,ISRAEL. NR 14 TC 4 Z9 4 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-6717 J9 HUM GENET JI Hum. Genet. PD AUG PY 1991 VL 87 IS 4 BP 506 EP 508 PG 3 WC Genetics & Heredity SC Genetics & Heredity GA GC432 UT WOS:A1991GC43200027 PM 1679035 ER PT J AU LONG, EO ROSENBRONSON, S KARP, DR MALNATI, M SEKALY, RP JARAQUEMADA, D AF LONG, EO ROSENBRONSON, S KARP, DR MALNATI, M SEKALY, RP JARAQUEMADA, D TI EFFICIENT CDNA EXPRESSION VECTORS FOR STABLE AND TRANSIENT EXPRESSION OF HLA-DR IN TRANSFECTED FIBROBLAST AND LYMPHOID-CELLS SO HUMAN IMMUNOLOGY LA English DT Article ID MAJOR HISTOCOMPATIBILITY COMPLEX; CLASS-II ANTIGENS; BETA-CHAIN GENE; T-CELLS; MONOCLONAL-ANTIBODIES; LYMPHOBLASTOID-CELLS; SODIUM-BUTYRATE; CLONES; RECOGNITION; HLA-DR1 AB cDNA expression vectors with several useful features were constructed. First, the long terminal repeat of Rous sarcoma virus was used as a promoter to obtain high levels of expression in various cells of human and mouse origin. Second, cis-linked expression units that confer resistance either to mycophenolic acid or the neomycin analog G418 were inserted to facilitate the isolation of transfected cells expressing the cDNA of interest. Third, by replicating in simian COS cells, these vectors can be used for efficient transient expression. cDNA fragments encoding the DR-alpha or DR-beta-chains of human class 11 major histocompatibility complex antigens were inserted into these vectors and high levels of cell surface HLA-DR antigen were obtained after cotransfection into mouse and human fibroblasts. These vectors were also successfully used to correct the inability of a class II-negative B cell line, derived from a patient with a congenital immunodeficiency, to present peptide antigen to DR-restricted T cells. C1 GEORGETOWN UNIV,SCH MED,DEPT PEDIAT,WASHINGTON,DC 20057. RP LONG, EO (reprint author), NIAID,IMMUNOGENET LAB,TWINBROOK-II FACIL,12441 PARKLAWN DR,ROCKVILLE,MD, USA. RI Long, Eric/G-5475-2011 OI Long, Eric/0000-0002-7793-3728 NR 37 TC 130 Z9 130 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0198-8859 J9 HUM IMMUNOL JI Hum. Immunol. PD AUG PY 1991 VL 31 IS 4 BP 229 EP 235 DI 10.1016/0198-8859(91)90092-N PG 7 WC Immunology SC Immunology GA FX052 UT WOS:A1991FX05200001 PM 1655683 ER PT J AU NEWMAN, BA YOUNGCOOPER, GO ALEXANDER, CB BECKER, RS KNIGHT, KL KELUS, AS MEIER, D MAGE, RG AF NEWMAN, BA YOUNGCOOPER, GO ALEXANDER, CB BECKER, RS KNIGHT, KL KELUS, AS MEIER, D MAGE, RG TI MOLECULAR ANALYSIS OF RECOMBINATION SITES WITHIN THE IMMUNOGLOBULIN HEAVY-CHAIN LOCUS OF THE RABBIT SO IMMUNOGENETICS LA English DT Article ID VARIABLE-REGION; VH GENES; IG-VH; EXPRESSION; ALLOTYPE; DNA; SEQUENCES; DELETION; CLONING; AGAROSE AB Previously, recombinations involving genes of the rabbit immunoglobulin heavy chain locus have been documented serologically. These data indicated that the sites at which the causative recombination events occurred could have been anywhere from within the V(H) gene cluster up to, or 3' of, C-mu. Since these sites could not be localized further by serological methods, we attempted to do this using techniques of molecular biology. DNAs from homozygous recombinant rabbits and from the appropriate non-recombinant parental haplotypes were characterized using Southern blots hybridized with a panel of probes derived from cloned regions of the rabbit immunoglobulin heavy chain gene complex. In all three recombinants, the site was downstream of the entire V(H) cluster and upstream of the J(H) cluster within an approximately 50 kilobase (kb) region containing expanses of repetitive-sequence DNA as well as D(H) genes. D(H)-specific probes further showed that in two of the recombinants, the recombination appears to have occurred within or 5' of D(H)1 and 5' of D(H)2 genes; in the third it occurred 3' of the D(H)2 genes but at least approximately 5 kb 5' of the J(H) region. C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. LOYOLA UNIV,STRITCH SCH MED,MAYWOOD,IL 60153. BASEL INST IMMUNOL,CH-4005 BASEL,SWITZERLAND. NR 46 TC 6 Z9 6 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0093-7711 J9 IMMUNOGENETICS JI Immunogenetics PD AUG PY 1991 VL 34 IS 2 BP 101 EP 109 PG 9 WC Genetics & Heredity; Immunology SC Genetics & Heredity; Immunology GA GA190 UT WOS:A1991GA19000004 PM 1678366 ER PT J AU DASTYCH, J COSTA, JJ THOMPSON, HL METCALFE, DD AF DASTYCH, J COSTA, JJ THOMPSON, HL METCALFE, DD TI MAST-CELL ADHESION TO FIBRONECTIN SO IMMUNOLOGY LA English DT Article ID MONOCLONAL-ANTIBODIES; EXTRACELLULAR-MATRIX; RECEPTOR; ATTACHMENT; EXPRESSION; MIGRATION; LAMININ; MACROPHAGES; FIBROBLASTS; MONOCYTES AB The MCP-5 murine mast cell line, as well as primary bone marrow-derived cultured mast cells (BMCMC), are demonstrated to bind to fibronectin, a ubiquitous adhesion protein of the extracellular matrix. BMCMC required activation by phorbol myristate acetate (PMA) to adhere to fibronectin, whereas MCP-5 displayed spontaneous adherence. The binding of both MCP-5 and BMCMC was dose dependent, with maximal adhesion at a fibronectin concentration of 20-mu-g ml. The 120,000 molecular weight (MW) proteolytic fragment of fibronectin containing the RGDS cell attachment site was able to substitute for the native fibronectin molecule in promoting mast cell attachment. Mast cell adhesion to fibronectin, in addition, could be inhibited by the RGDS peptide alone. These data suggest that, in addition to the previously described mast cell-laminin interactions, mast cells also adhere to fibronectin, thus providing further insight into their tissue localization and possible roles in processes such as wound healing and fibrosis. C1 NIAID,CLIN INVEST LAB,MAST CELL PHYSIOL SECT,BLDG 10,ROOM 11C210,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 30 TC 71 Z9 71 U1 1 U2 1 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0019-2805 J9 IMMUNOLOGY JI Immunology PD AUG PY 1991 VL 73 IS 4 BP 478 EP 484 PG 7 WC Immunology SC Immunology GA GA778 UT WOS:A1991GA77800019 PM 1916899 ER PT J AU SEMON, BA PHANG, JM AF SEMON, BA PHANG, JM TI ACCUMULATION OF PYRROLINE 5-CARBOXYLIC ACID IN CONDITIONED MEDIUM OF CULTURED FIBROBLAST - STIMULATORY EFFECTS OF SERUM, INSULIN, AND IGF-1 SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY LA English DT Article DE PYRROLINE-5-CARBOXYLIC ACID; FIBROBLAST; INSULIN; INSULIN-LIKE GROWTH FACTOR-I; FETAL BOVINE SERUM; CELL CULTURE ID PURINE NUCLEOTIDE PRODUCTION; PYRROLINE-5-CARBOXYLIC ACID; HUMAN-ERYTHROCYTES; PENTOSE PATHWAY; PLASMA AB Pyrroline 5-carboxylate, an intermediate of amino acid metabolism, is released into medium by cultured normal human fibroblasts. With cells made quiescent by serum starvation, the addition of 10% fetal bovine serum augmented the release of pyrroline 5-carboxylate into medium by 2.5-fold. Although platelet-derived growth factor was without effect, both insulin and insulinlike growth factor-1 nearly reproduced the serum effect. The dose-dependence of insulin and insulinlike growth factor 1 effects suggested their mediation by their own respective receptors. Although the mechanism for the stimulatory effect remains unknown, these effects of insulin and insulinlike growth factor 1 on pyrroline 5-carboxylate suggest hormonal regulation of pyrroline 5-carboxylate release. C1 NCI,FREDERICK CANC RES & DEV CTR,DIV CANC PREVENT & CONTROL,NUTR & MOLEC REGULAT LAB,BLDG 560,FREDERICK,MD 21702. NR 16 TC 6 Z9 6 U1 1 U2 2 PU SOC IN VITRO BIOLOGY PI COLUMBIA PA 8815 CENTRE PARK DRIVE SUITE 210, COLUMBIA, MD 21045 SN 0073-5655 J9 IN VITRO CELL DEV B PD AUG PY 1991 VL 27 IS 8 BP 665 EP 669 PG 5 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA GG585 UT WOS:A1991GG58500013 ER PT J AU DEJONG, DM PATE, JL KIRKLAND, TN TAYLOR, CE BAKER, PJ TAKAYAMA, K AF DEJONG, DM PATE, JL KIRKLAND, TN TAYLOR, CE BAKER, PJ TAKAYAMA, K TI LIPOPOLYSACCHARIDELIKE IMMUNOLOGICAL PROPERTIES OF CELL-WALL GLYCOPROTEINS ISOLATED FROM CYTOPHAGA-JOHNSONAE SO INFECTION AND IMMUNITY LA English DT Article ID III PNEUMOCOCCAL POLYSACCHARIDE; ANTIBODY-RESPONSE; OUTER-MEMBRANE; CAPNOCYTOPHAGA-OCHRACEA; LIPID-A; CHEMICAL CHARACTERIZATION; SALMONELLA-TYPHIMURIUM; LYMPHOCYTE MITOGEN; MASS-SPECTROMETRY; ESCHERICHIA-COLI AB Glycoproteins (GP) previously shown to be involved in the gliding motility of Cytophaga johnsonae were examined for biological activities characteristic of lipopolysaccharide (LPS). These integral membrane proteins activated 70Z/3 pre-B cells to synthesize immunoglobulin M, induced B cells to synthesize non-antigen-specific polyclonal immunoglobulin, induced macrophages to produce tumor necrosis factor, and modulated the antibody response to type III pneumococcal polysaccharide in the absence of thymus-derived (T) lymphocytes. Except for the GP activity in the 70Z/3 assay, all activities of the GP were comparable to or greater than those of LPS. No LPS was detected in the preparations of GP used or in the phenol-water extracts of C. johnsonae. The mechanism by which these GP exerted their biological activities was distinct from that of LPS, since LPS-resistant C3H/HeJ mice responded to GP. Furthermore, biologically inactive diphosphoryl lipid A obtained from nontoxic LPS of Rhodopseudomonas sphaeroides (an analog of toxic lipid A), which is an antagonist of LPS, did not block the induction of tumor necrosis factor by GP in macrophages. These results showed that the cell surface GP from C. johnsonae are potent LPS-like activators of B cells and macrophages. We suggest that these GP might be good candidates for use in developing an effective adjuvant system. C1 UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BACTERIOL,MADISON,WI 53706. UNIV CALIF SAN DIEGO,DEPT PATHOL & MED,SAN DIEGO,CA 92103. VET AFFAIRS MED CTR,DIV INFECT DIS,SAN DIEGO,CA 92161. NIAID,TWINBROOK II RES FACIL,IMMUNOGENET LAB,ROCKVILLE,MD 20852. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MYCOBACTERIOL RES LAB,MADISON,WI 53705. FU NIGMS NIH HHS [GM-36054] NR 47 TC 4 Z9 4 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD AUG PY 1991 VL 59 IS 8 BP 2631 EP 2637 PG 7 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA FY331 UT WOS:A1991FY33100018 PM 1855983 ER PT J AU SU, H CALDWELL, HD AF SU, H CALDWELL, HD TI INVITRO NEUTRALIZATION OF CHLAMYDIA-TRACHOMATIS BY MONOVALENT FAB ANTIBODY SPECIFIC TO THE MAJOR OUTER-MEMBRANE PROTEIN SO INFECTION AND IMMUNITY LA English DT Note ID PROTECTIVE MONOCLONAL-ANTIBODIES AB Monovalent Fab antibodies to serovar- and subspecies-specific epitopes of the major outer membrane protein (MOMP) of Chlamydia trachomatis neutralized infectivity for hamster kidney cells by preventing chlamydial attachment. These findings exclude the aggregation of chlamydiae as a mechanism of anti-MOMP neutralization and provide additional evidence in support of the MOMP as a chlamydial adhesin. C1 NIAID,ROCKY MT LABS,INTRACELLULAR PARASITES LAB,HAMILTON,MT 59840. NR 9 TC 46 Z9 47 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD AUG PY 1991 VL 59 IS 8 BP 2843 EP 2845 PG 3 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA FY331 UT WOS:A1991FY33100048 PM 1713202 ER PT J AU GREM, JL KING, SA SORENSEN, JM CHRISTIAN, MC AF GREM, JL KING, SA SORENSEN, JM CHRISTIAN, MC TI CLINICAL USE OF THYMIDINE AS A RESCUE AGENT FROM METHOTREXATE TOXICITY SO INVESTIGATIONAL NEW DRUGS LA English DT Article DE HIGH-DOSE METHOTREXATE; THYMIDINE RESCUE ID HIGH-DOSE METHOTREXATE; CITROVORUM FACTOR RESCUE; PHASE-I; OSTEO-SARCOMA; LEUCOVORIN; PROTECTION; INFUSIONS; HEMODIALYSIS; LEUKEMIA; THERAPY AB Thymidine has been available for clinical research as a rescue agent since 1978 under sponsorship of the Division of Cancer Treatment, National Cancer Institute. Renal insufficiency following administration of high dose methotrexate results in prolonged exposure to toxic concentrations of drug. Thymidine has been used in conjunction with leucovorin and alkaline hydration to protect patients with acute renal dysfunction from life-threatening methotrexate toxicity. The outcome of eight cases in which thymidine was released under the special exception mechanism to treat patients who developed acute renal failure following methotrexate are reported. The clinical trials using thymidine in combination with methotrexate in patients with normal renal function are also reviewed. C1 NCI,DIV CANC TREATMENT,CANC THERAPY EVALUAT PROGRAM,INVEST DRUG BRANCH,BETHESDA,MD 20892. RP GREM, JL (reprint author), NCI,DIV CANC TREATMENT,CLIN ONCOL PROGRAM,BLDG 10,RM 12N226,BETHESDA,MD 20892, USA. NR 43 TC 17 Z9 17 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0167-6997 J9 INVEST NEW DRUG JI Invest. New Drugs PD AUG PY 1991 VL 9 IS 3 BP 281 EP 290 PG 10 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA FV701 UT WOS:A1991FV70100012 PM 1838364 ER PT J AU MASER, JD KAELBER, C WEISE, RE AF MASER, JD KAELBER, C WEISE, RE TI INTERNATIONAL USE AND ATTITUDES TOWARD DSM-III AND DSM-III-R - GROWING CONSENSUS IN PSYCHIATRIC CLASSIFICATION SO JOURNAL OF ABNORMAL PSYCHOLOGY LA English DT Article ID MULTIAXIAL SYSTEM; POLITICS; TRUTH; GO C1 NIMH,DIV CLIN RES,ROCKVILLE,MD 20857. NR 32 TC 75 Z9 77 U1 0 U2 1 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0021-843X J9 J ABNORM PSYCHOL JI J. Abnorm. Psychol. PD AUG PY 1991 VL 100 IS 3 BP 271 EP 279 DI 10.1037//0021-843X.100.3.271 PG 9 WC Psychology, Clinical; Psychology, Multidisciplinary SC Psychology GA FY376 UT WOS:A1991FY37600004 PM 1918604 ER PT J AU WEEKS, BS KLOTMAN, ME DHAWAN, S KIBBEY, M RAPPAPORT, J KLEINMAN, HK YAMADA, KM KLOTMAN, PE AF WEEKS, BS KLOTMAN, ME DHAWAN, S KIBBEY, M RAPPAPORT, J KLEINMAN, HK YAMADA, KM KLOTMAN, PE TI HIV-1 INFECTION OF HUMAN LYMPHOCYTES-T RESULTS IN ENHANCED ALPHA-5-BETA-1 INTEGRIN EXPRESSION SO JOURNAL OF CELL BIOLOGY LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; EXTRACELLULAR-MATRIX COMPONENTS; ADHESION MOLECULE-1 ICAM-1; HUMAN-PLASMA FIBRONECTIN; CELL-SURFACE RECEPTORS; MONOCLONAL-ANTIBODIES; KAPOSIS-SARCOMA; HOMING RECEPTOR; ENDOTHELIAL-CELLS; VLA PROTEINS AB Altered T cell adherence after human immunodeficiency virus 1 (HIV-1) infection may contribute to viral pathogenesis in the acquired immune deficiency syndrome. To address this hypothesis, we assessed mechanisms of T cell adherence to extracellular matrix proteins in vitro. We found that after HIV-1 infection, both chronically infected H9 CD4+ T cells and acutely infected primary peripheral blood lymphocytes acquired the ability to adhere to the extracellular matrix glycoprotein fibronectin, to a lesser extent to type IV collagen and laminin, but not to type I collagen. H9 cells chronically infected with two of the three HIV-1 strains studied showed approximately a sevenfold increase in attachment to fibronectin, while the same cells infected with the human retrovirus HIV-2 did not. Adhesion was accompanied by changes in morphology, including marked spreading and increased filopodia. These alterations were not blocked by the protein kinase C inhibitor H-7, which did inhibit TPA-induced T cell attachment to fibronectin. Monoclonal antibodies against both the alpha-5 and the beta-1 subunits of the classical fibronectin receptor as well as an Arg-Gly-Asp (RGD) peptide inhibited attachment, whereas anti-alpha-4 monoclonal antibodies and the CS1 peptide did not. Binding to collagen IV was also inhibited by the anti-beta-1 monoclonal antibody, but not the other antibodies. Cells metabolically labeled with [S-35]methionine and analyzed by immunoprecipitation with polyclonal anti-beta-1 integrin antibody showed a 2.5-fold increase in integrin synthesis in infected cells compared to uninfected controls. This increase in synthesis was associated with an increase in cell surface expression of both alpha-5 and beta-1 integrins by FACS(R) (registered trademark of Becton Dickinson for a fluorescence-activated cell sorter) analysis. Enhanced expression of integrins such as alpha-5-beta-1 may cause T cell adherence to a variety of tissues, where released viral gene products may induce some of the tissue-specific manifestations of HIV-1 infection. C1 NCI,ORAL MED LAB,BETHESDA,MD 20892. NIDR,ORAL MED LAB,BETHESDA,MD 20892. RP WEEKS, BS (reprint author), NIDR,DEV BIOL LAB,BETHESDA,MD 20892, USA. RI klotman, mary/A-1921-2016 NR 62 TC 39 Z9 39 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9525 J9 J CELL BIOL JI J. Cell Biol. PD AUG PY 1991 VL 114 IS 4 BP 847 EP 853 DI 10.1083/jcb.114.4.847 PG 7 WC Cell Biology SC Cell Biology GA GB207 UT WOS:A1991GB20700023 PM 1831204 ER PT J AU TENDLER, CL GREENBERG, SJ BURTON, JD DANIELPOUR, D KIM, SJ BLATTNER, WA MANNS, A WALDMANN, TA AF TENDLER, CL GREENBERG, SJ BURTON, JD DANIELPOUR, D KIM, SJ BLATTNER, WA MANNS, A WALDMANN, TA TI CYTOKINE INDUCTION IN HTLV-I ASSOCIATED MYELOPATHY AND ADULT T-CELL LEUKEMIA - ALTERNATE MOLECULAR MECHANISMS UNDERLYING RETROVIRAL PATHOGENESIS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Article DE GAMMA-INTERFERON; TUMOR NECROSIS FACTOR-ALPHA; INTERLEUKIN-1-BETA; TRANSFORMING GROWTH FACTOR-BETA-1; IMMUNOSUPPRESSION ID TUMOR NECROSIS FACTOR; VIRUS TYPE-I; TROPICAL SPASTIC PARAPARESIS; GROWTH FACTOR-BETA; COLONY-STIMULATING FACTOR; IMMUNE INTERFERON; INTERLEUKIN-2 RECEPTOR; EXPRESSION; ACTIVATION; GENE AB The human T-cell lymphotropic virus type I (HTLV-I) is capable of inducing a variety of host cellular genes including many of the cytokines responsible for immune regulation and osteoclast activation. This derangement in cytokine expression may contribute to the panoply of disease states associated with HTLV-I infection such as the adult T-cell leukemia (ATL) and HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP). We wished to determine if there was a correlation between the expression of an array of cytokines and the diverse clinical manifestations of ATL and HAM/TSP. Utilizing the techniques of specific mRNA amplification by the polymerase chain reaction (PCR) as well as Northern blotting, we analyzed the ex vivo mRNA expression of gamma-interferon (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin-1-beta (IL-1-beta), and transforming growth factor-beta-1 (TGF-beta-1) in the peripheral blood of HAM/TSP and ATL patients as well as asymptomatic seropositive carriers. IFN-gamma, TNF-alpha, and IL-1-beta transcripts were up-regulated in patients with HAM/TSP and seropositive carriers when compared to their levels in ATL and normal controls. In contrast, the ATL patients constitutively expressed higher levels of TGF-beta-1 mRNA than HAM/TSP and seropositive carriers. In addition, TNF-alpha and IL-1-beta serum levels were elevated in HAM/TSP, but not in ATL patients nor seropositive carriers. However, the circulating leukemic cells from ATL patients secreted increased levels of TGF-beta-1 protein into the culture medium than T-cells derived from HAM/TSP patients. Collectively these results suggest that induction of IFN-gamma, TNF-alpha, and IL-1-beta in HAM/TSP may initiate an inflammatory cascade with subsequent events leading to immune mediated destruction of the central nervous system in these patients. Expression of osteoclast activators such as TNF-alpha and IL-1-beta is not associated with hypercalcemia in ATL. Finally, impaired cellular and humoral immune responses present in ATL, but not in HAM/TSP, may be related to elevated levels of TGF-beta-1 produced by the leukemic cells. These differences in retroviral-induced host cytokine expression in ATL and HAM/TSP suggest alternate roles in disease pathogenesis. C1 NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. NCI,METAB BRANCH,BETHESDA,MD 20892. NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. NR 50 TC 104 Z9 104 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD AUG PY 1991 VL 46 IS 4 BP 302 EP 311 DI 10.1002/jcb.240460405 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA GA399 UT WOS:A1991GA39900004 PM 1757474 ER PT J AU KINDY, MS BROWN, KE SONENSHEIN, GE AF KINDY, MS BROWN, KE SONENSHEIN, GE TI REGULATION OF EXPRESSION OF THE GROWTH-STATE-RELATED GENES 2F1 AND 2A9 DURING ENTRY OF QUIESCENT SMOOTH-MUSCLE CELLS INTO THE CELL-CYCLE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Article DE SMCS; MESSENGER RNA; MITOCHONDRIAL ADP/ATP CARRIER; CALCYLIN; CELL CYCLE ID MESSENGER-RNA; MOLECULAR-CLONING; CALCYCLIN; ONCOGENE AB Vascular smooth muscle cells (SMCs) play a key role in the development of major arteries. Furthermore, abnormal growth of vascular smooth muscle cells has been implicated in the progression of major diseases of the cardiovascular system. Here, we report detection in primary cultures of bovine vascular smooth muscle cells of mRNA for two growth-state-related genes, 2F1 and 2A9, which code for a mitochondrial ADP/ATP carrier and calcyclin, respectively, and on the characterization of their cell cycle expression. Cultures of exponentially growing smooth muscle cells were made quiescent by serum deprivation. Upon readdition of serum, cells entered the cell cycle synchronously; DNA synthesis began 12 h post-serum addition. Levels of 2F1 and 2A9 RNA were low in quiescent cells and increased between 2 and 4 h post-serum addition. No changes in the rates of transcription of the 2F1 or 2A9 genes were detected by nuclear run-off assays during the time course. Thus the regulation of changes in expression of 2F1 and 2A9 in early G1 is mediated post-transcriptionally. C1 BOSTON UNIV,SCH MED,DEPT BIOCHEM,BOSTON,MA 02118. UNIV KENTUCKY,DEPT BIOCHEM,LEXINGTON,KY 40536. NIDR,DEV BIOL LAB,BETHESDA,MD 20892. FU NHLBI NIH HHS [HL13262, T32 HL07429] NR 19 TC 16 Z9 16 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD AUG PY 1991 VL 46 IS 4 BP 345 EP 350 DI 10.1002/jcb.240460409 PG 6 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA GA399 UT WOS:A1991GA39900008 PM 1757477 ER PT J AU DANIELPOUR, D KIM, KY WINOKUR, TS SPORN, MB AF DANIELPOUR, D KIM, KY WINOKUR, TS SPORN, MB TI DIFFERENTIAL REGULATION OF THE EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA-S-1 AND FACTOR-BETA-2 BY RETINOIC ACID, EPIDERMAL GROWTH-FACTOR, AND DEXAMETHASONE IN NRK-49F AND A549 CELLS SO JOURNAL OF CELLULAR PHYSIOLOGY LA English DT Article ID FACTOR-BETA; 2 FORMS; RECEPTOR; KERATINOCYTES; FIBROBLASTS; INHIBITION; TGF-BETA-1; INDUCTION; BINDING; TISSUES AB Although most biological activities of transforming growth factor-betas 1 and 2 (TGF-beta-1 and TGF-beta-2) examined in vitro are similar or identical, recent studies suggest that each of these factors may be independently regulated in vivo. In this study we have used highly sensitive and specific sandwich enzyme-linked immunosorbent assays for TGF-beta-1 and TGF-beta-2 to examine the effects of a variety of treatments on expression of these two TGF-beta isoforms. We show that epidermal growth factor (EGF) induces secretion of TGF-beta-1 and not TGF-beta-2, whereas retinoic acid (RA) induces secretion of TGF-beta-2 and not TGF-beta-1 in NRK-49F normal rat kidney fibroblasts and A549 human lung carcinoma cells. Moreover, treatment with EGF diminishes the levels of TGF-beta-2, while RA decreases the levels of TGF-beta-1 in both cell lines. Dexamethasone (Dex), on the other hand, inhibits the secretion of both TGF-beta-1 and TGF-beta-2 in A549 cells, while selectively inhibiting TGF-beta-1 secretion in NRK-49F cells. The interactive effects of EGF, RA, and Dex on the production of TGF-beta-1 and TGF-beta-2, which were studied on NRK-49F cells, demonstrate that EGF blocks the induction of TGF-beta-2 mRNA and peptide by RA, while Dex inhibits the induction of TGF-beta-1 mRNA and peptide by EGF. These results demonstrate that RA, EGF and Dex are each unique, differential, and interactive regulators of the expression of TGF-betas 1 and 2. RP DANIELPOUR, D (reprint author), NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892, USA. NR 40 TC 80 Z9 80 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0021-9541 J9 J CELL PHYSIOL JI J. Cell. Physiol. PD AUG PY 1991 VL 148 IS 2 BP 235 EP 244 DI 10.1002/jcp.1041480208 PG 10 WC Cell Biology; Physiology SC Cell Biology; Physiology GA GF533 UT WOS:A1991GF53300007 PM 1880152 ER PT J AU MCDIARMID, R AF MCDIARMID, R TI ON THE ABSORPTION-SPECTRUM OF THE 3S RYDBERG STATE OF ACETONE SO JOURNAL OF CHEMICAL PHYSICS LA English DT Article ID PHOTODISSOCIATION; DYNAMICS AB The optical absorption spectra of acetone and acetone-d6 in the 3s Rydberg region have been remeasured and reanalyzed. Activity is observed in the a1 CH3 deformation, CH3 rock, and CC stretching vibrations, as before. The two low frequency displacement subbands of the transition, + 250 and + 309 cm-1 have been reassigned; the former is twice the a2 CH3 torsion, the latter cannot be assigned. Possible photophysical implications of the large frequency shifts in the methyl-centered modes are discussed. RP MCDIARMID, R (reprint author), NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892, USA. NR 17 TC 32 Z9 32 U1 0 U2 4 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0021-9606 J9 J CHEM PHYS JI J. Chem. Phys. PD AUG 1 PY 1991 VL 95 IS 3 BP 1530 EP 1536 DI 10.1063/1.461067 PG 7 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA GA106 UT WOS:A1991GA10600012 ER PT J AU MCDIARMID, R GEDANKEN, A AF MCDIARMID, R GEDANKEN, A TI ASSIGNMENT OF FRANCK-CONDON ENABLED VIBRATIONS IN THE NV1[-X TRANSITION OF CYCLOPENTADIENE SO JOURNAL OF CHEMICAL PHYSICS LA English DT Note ID ABSORPTION-SPECTRUM; EXCITED-STATES; BUTADIENE; 1,3-BUTADIENE; SURFACES C1 BAR ILAN UNIV,DEPT CHEM,IL-52100 RAMAT GAN,ISRAEL. RP MCDIARMID, R (reprint author), NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892, USA. NR 16 TC 4 Z9 4 U1 1 U2 2 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0021-9606 J9 J CHEM PHYS JI J. Chem. Phys. PD AUG 1 PY 1991 VL 95 IS 3 BP 2220 EP 2221 DI 10.1063/1.460975 PG 2 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA GA106 UT WOS:A1991GA10600091 ER PT J AU ROSENTHAL, NE AF ROSENTHAL, NE TI PLASMA MELATONIN AS A MEASURE OF THE HUMAN CLOCK SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Editorial Material ID LIGHT RP ROSENTHAL, NE (reprint author), NIMH, CLIN PSYCHOBIOL BRANCH, ENVIRONM PSYCHIAT SECT, BLDG 10-4S-239, 9000 ROCKVILLE PIKE, BETHESDA, MD 20892 USA. NR 7 TC 36 Z9 36 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD AUG PY 1991 VL 73 IS 2 BP 225 EP 226 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY511 UT WOS:A1991FY51100002 PM 1856257 ER PT J AU SMOAK, B DEUSTER, P RABIN, D CHROUSOS, G AF SMOAK, B DEUSTER, P RABIN, D CHROUSOS, G TI CORTICOTROPIN-RELEASING HORMONE IS NOT THE SOLE FACTOR MEDIATING EXERCISE-INDUCED ADRENOCORTICOTROPIN RELEASE IN HUMANS SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID ARGININE VASOPRESSIN; ANTERIOR-PITUITARY; SECRETION; RESPONSES; PLASMA; BASAL; CELLS; ACTH; IMMUNONEUTRALIZATION; MECHANISMS AB To determine whether CRH is the sole mediator of ACTH release during exercise, five men and five women were given, in a subject-blinded random manner at separate visits, both a 6-h infusion of ovine CRH (1-mu-g/kg.h) and a saline infusion as a placebo. After the fourth hour of each infusion, when plasma concentrations of ovine CRH were sufficiently elevated to saturate the capacity of the corticotroph to respond further to CRH, each subject completed a high intensity intermittent run. Plasma ACTH and cortisol levels increased significantly during the CRH infusion from 4.6 +/- 0.8 (mean +/- SE) to 8.6 +/- 1.6 pmol/L and from 361 +/- 39 to 662 +/- 70 nmol/L, respectively (P < 0.05). Despite elevated preexercise cortisol levels during the CRH infusion, plasma ACTH rose to 32.0 +/-8.5 pmol/L after exercise. During the saline infusion, plasma ACTH rose from 3.4 +/- 0.6 pmol/L before exercise to 18.1 +/- 4.2 after exercise. Time-integrated responses for postexercise values of ACTH and cortisol were higher during the CRH infusion than during the saline infusion (P < 0.05). No significant exercise-induced differences in heart rate or plasma concentrations of lactate, epinephrine, and norepinephrine were observed between the two tests. The findings suggest that some factor(s) in addition to CRH causes ACTH release during exercise. Vasopressin, produced by the magnocellular and/or parvocellular neurons of the hypothalamus, is a likely candidate. C1 UNIFORMED SERV UNIV HLTH SCI, DEPT MIL MED, 4301 JONES BRIDGE RD, BETHESDA, MD 20814 USA. NICHHD, DEV ENDOCRINOL BRANCH, BETHESDA, MD 20892 USA. RI Deuster, Patricia/G-3838-2015 OI Deuster, Patricia/0000-0002-7895-0888 NR 27 TC 32 Z9 32 U1 0 U2 2 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD AUG PY 1991 VL 73 IS 2 BP 302 EP 306 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY511 UT WOS:A1991FY51100014 PM 1649838 ER PT J AU MALOZOWSKI, S HAO, EH REN, SG MARIN, G LIU, L SOUTHERS, JL MERRIAM, GR AF MALOZOWSKI, S HAO, EH REN, SG MARIN, G LIU, L SOUTHERS, JL MERRIAM, GR TI GROWTH-HORMONE (GH) RESPONSES TO THE HEXAPEPTIDE GH-RELEASING PEPTIDE AND GH-RELEASING HORMONE (GHRH) IN THE CYNOMOLGUS MACAQUE - EVIDENCE FOR NON-GHRH-MEDIATED RESPONSES SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID SYNTHETIC HEXAPEPTIDE; PITUITARY-CELLS; INVIVO ACTIVITY; INVITRO; SOMATOSTATIN; PROPRANOLOL; SECRETION; PROLACTIN; RATS AB GH-releasing peptide (GHRP; His-D-Trp-Ala-Trp-D-Phe-Lys-NH2), a hexapeptide derived from enkephalin, has been shown to have GH-releasing activity in man and several animal species. To characterize the GHRP dose-response curve and compare it with that of GH-releasing hormone [GHRH-(1-44)NH2], six unanesthetized young adult cynomolgus macaques were tested with a range of iv doses of GHRP or GHRH in random order. Animals were fitted with vests and tethers. Blood samples were obtained before and at 15-min intervals after the administration of drugs. Doses ranged from 0.03-3 mg/kg for GHRP and from 1-30-mu-g/kg for GHRH. The dose-response curves for the two peptides were not parallel. GHRP had lower potency, but evoked a much higher peak GH response than GHRH (> 55 vs. 12-mu-g/L). Because one of the proposed mechanisms of action of GHRP is the inhibition of somatostatin (SS), we tested the effects of propranolol, which inhibits SS, on the GH responses to GHRH and GHRP. Propranolol was given at a dose of 14-mu-g/kg, iv, 10 min before the injection of saline, GHRH (10-mu-g/kg), or GHRP (1 mg/kg). GH responses to propranolol alone did not differ from those to placebo (peak, 6 +/- 2 vs. 8 +/- 2-mu-g/L). However, propranolol pretreatment doubled the GH responses to both GHRH and GHRP compared with those to GHRH or GHRP alone 28 +/- 5-mu-g/L vs. 14 +/- 5 (P < 0.05) and 54 +/- 2 vs. 25 +/- 6-mu-g/L (P < 0.001), respectively]. These results show that GHRP causes a potent dose-dependent release of GH in this primate species. Since GHRP can produce a greater maximal GH response than GHRH, mechanisms other than release of endogenous GHRH must be involved. C1 NIH, DIV RES SERV, VET RESOURCES BRANCH, BETHESDA, MD 20892 USA. RP MALOZOWSKI, S (reprint author), NICHHD, DEV ENDOCRINOL BRANCH, BLDG 10, ROOM 10N262, BETHESDA, MD 20892 USA. NR 24 TC 64 Z9 64 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD AUG PY 1991 VL 73 IS 2 BP 314 EP 317 PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY511 UT WOS:A1991FY51100016 PM 1856262 ER PT J AU ROSS, JL LONG, LM FEUILLAN, P CASSORLA, F CUTLER, GB AF ROSS, JL LONG, LM FEUILLAN, P CASSORLA, F CUTLER, GB TI NORMAL BONE-DENSITY OF THE WRIST AND SPINE AND INCREASED WRIST FRACTURES IN GIRLS WITH TURNERS SYNDROME SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID GROWTH-HORMONE; WOMEN; DEMINERALIZATION; PREMENOPAUSAL; MASS; AGE AB Turner's syndrome is associated with multiple skeletal abnormalities, including osteoporosis. We evaluated the hypothesis that girls with Turner's syndrome may have deficient bone density before the expected age of pubertal onset (9-13 yr) by comparing the bone mineral content of the wrist and lumbar spine in 78 girls with Turner's syndrome (4-13 yr old) and 28 normal prepubertal girls who were matched for age, bone age, body mass index, or height age. The bone mineral content of the wrist and spine was measured by single photon absorptiometry (SPA) and dual photon absorptiometry (DPA), respectively. SPA values for girls with Turner's syndrome vs. normal subjects (4-6.9, 7-9.9, and 10-12.9 yr old) were (mean +/- SD) 0.27 +/- 0.05 vs. 0.36 +/- 0.05, 0.35 +/- 0.06 vs. 0.41 +/- 0.06, and 0.41 +/- 0.05 vs. 0.45 +/- 0.03 g/cm2, respectively. SPA values in the Turner's syndrome girls were decreased compared to those in normal prepubertal girls, matched for age (P < 0.0001), bone age, (P < 0.001), and body mass index (BMI; P < 0.0001), but not for height age. DPA values for girls with Turner's syndrome vs. normal girls in the same age categories were 0.65 +/- 0.06 vs. 0.70 +/- 0.09, 0.77 +/- 0.08 vs. 0.79 +/- 0.09, and 0.83 +/- 0.12 vs. 0.78 +/-0.07 g/cm2. DPA values in Turner's syndrome girls (as a group) were decreased compared to those in normal prepubertal girls matched for age (P < 0.05) and BMI (P < 0.02), but not for bone age or height age. The annual incidence rate of wrist fractures in Turner's syndrome girls (9.1 of 1000) was significantly increased compared to the reported annual incidence rate in normal children (3.5 of 1000; P < 0.003). We conclude that prepubertal-aged girls with Turner's syndrome (< 13 yr old) have normal bone density for height age, but significantly decreased bone density of the wrist for chronological age, bone age, and BMI. They also have significantly more wrist fractures than normal girls, but it is not clear that this is related to their bone density. C1 NICHHD, DEV ENDOCRINOL BRANCH, BETHESDA, MD 20892 USA. RP ROSS, JL (reprint author), MED COLL PENN, DEPT PEDIAT, 3300 HENRY AVE, PHILADELPHIA, PA 19129 USA. NR 20 TC 78 Z9 79 U1 0 U2 1 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD AUG PY 1991 VL 73 IS 2 BP 355 EP 359 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY511 UT WOS:A1991FY51100022 PM 1856266 ER PT J AU LAUE, L PECK, GL LORIAUX, DL GALLUCCI, W CHROUSOS, GP AF LAUE, L PECK, GL LORIAUX, DL GALLUCCI, W CHROUSOS, GP TI ADRENAL ANDROGEN SECRETION IN POSTADOLESCENT ACNE - INCREASED ADRENOCORTICAL FUNCTION WITHOUT HYPERSENSITIVITY TO ADRENOCORTICOTROPIN SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID PRIMARY CORTISOL RESISTANCE; PLASMA ANDROGENS; WOMEN; RECEPTOR; VULGARIS; RADIOIMMUNOASSAY; TESTOSTERONE; HIRSUTISM; HORMONE; EXCESS AB Basal and ACTH-stimulated plasma levels of cortisol, DELTA-4-androstenedione, and dehydroepiandrosterone (DHEA) were measured in a group of 11 female patients with postadolescent acne resistant to or relapsing after conventional therapy and in a group of 10 normal women without acne or hirsutism. Each patient received, in a blinded random fashion, a series of 5 1-h ACTH tests. For each test a different dose of ACTH-(1-24) was administered, ranging from 0-1-mu-g/kg, given as an iv bolus. Blood samples were collected 0, 10, 30, and 60 min after ACTH bolus injection. Patients with acne had slightly higher concentrations of basal cortisol, DELTA-4-androstenedione, and DHEA than normal controls (P < 0.05). After ACTH-(1-24) stimulation, the same patients had greater peak and time-integrated DHEA concentrations (P < 0.03). The ED50 values of the cortisol dose-response curves were similar in patients and normal women (P < 0.05), suggesting that there are no differences in the sensitivity of the adrenal cortex to ACTH between the acne patients and the controls studied. The ratio of DHEA to cortisol response was significantly elevated in women with acne compared to that in control women, suggesting some preponderance of the DELTA-5 pathway of steroidogenesis in acne (P < 0.05). These findings of basal and ACTH-stimulated hypersecretion of DELTA-5-androgens in patients with postadolescent acne are consistent with an increased volume of androgen-secreting tissue, rather than hypersensitivity of the adrenal zona reticularis to ACTH. C1 NICHHD, DEV ENDOCRINOL BRANCH, BETHESDA, MD 20892 USA. NCI, DERMATOL BRANCH, BETHESDA, MD 20892 USA. RP LAUE, L (reprint author), GEORGETOWN UNIV, CHILDRENS MED CTR, DEPT PEDIAT, 3800 RESERVOIR RD NW, WASHINGTON, DC 20007 USA. NR 29 TC 23 Z9 23 U1 0 U2 1 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD AUG PY 1991 VL 73 IS 2 BP 380 EP 384 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY511 UT WOS:A1991FY51100026 PM 1830321 ER PT J AU ROSE, SR MUNICCHI, G BARNES, KM KAMP, GA URIARTE, MM ROSS, JL CASSORLA, F CUTLER, GB AF ROSE, SR MUNICCHI, G BARNES, KM KAMP, GA URIARTE, MM ROSS, JL CASSORLA, F CUTLER, GB TI SPONTANEOUS GROWTH-HORMONE SECRETION INCREASES DURING PUBERTY IN NORMAL GIRLS AND BOYS SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID ISOLATED HYPOGONADOTROPIC HYPOGONADISM; INTEGRATED CONCENTRATION; PRECOCIOUS PUBERTY; PULSE AMPLITUDE; SHORT STATURE; CHILDREN; AGE; DEFICIENCY; DYNAMICS; OBESITY AB To test the hypothesis that GH secretion increases during puberty, we measured GH levels in samples obtained every 20 min for 24 h from 132 normal children and adolescents. In both girls and boys, GH levels increased during puberty. The increase in mean levels was earlier in girls than boys, was most evident at night, and was due to increased pulse amplitude rather than a change in pulse frequency. The mean nighttime GH level in girls with bone ages (BA) greater than 12 to 14 yr were significantly greater than the mean level in girls with BA less than 8 yr (7.3 +/- 3.0 vs. 3.4 +/- 1.7-mu-g/L; P < 0.01) and were greatest at breast stage 3 (7.9 +/- 2.5-mu-g/L). GH pulse amplitude increased significantly before pubertal onset in girls and was significantly greater at BA greater than 12 to 14 yr than at BA of 8 yr or less (13.9 +/- 6.0 vs. 7.9 +/- 4.8-mu-g/L; P < 0.01) and greatest at breast stage 3 (15.0 +/- 6.3-mu-g/L). The pubertal increase in GH secretion was delayed in boys compared to girls, with the lowest mean 24-h GH and mean nighttime GH values in boys with BA greater than 8 to 11 yr. The mean nighttime GH level at BA greater than 11 to 13 yr in boys was significantly greater than that in the boys with BA greater than 8 to 11 yr (5.8 +/- 2.9 vs. 3.5 +/- 2.1-mu-g/L; P < 0.05) and was greatest at a testicular volume of more than 10 to 15 mL (6.5 +/- 2.0-mu-g/L). The mean nighttime GH pulse amplitude in boys was significantly greater at BA greater than 11 to 13 yr than at BA greater than 8 to 11 yr (13.9 +/- 5.7 vs. 7.3 +/- 2.6-mu-g/L, P < 0.05) and was greatest at a testicular volume greater than 20 mL (15.8 +/- 12.0-mu-g/L). The mean nighttime GH levels correlated inversely with body mass index in both sexes, although the correlation achieved statistical significance only for the girls, being stronger in breast stage 3 to 5 girls (r = -0.57 P = 0.0007; n = 32) than in stage 1 and 2 girls (r = -0.38; P = 0.03; n = 32). These observations in normal adolescents emphasize the importance of interpreting spontaneous GH levels in short children in relation to normative data appropriate for sex, body mass, and bone age or pubertal stage. C1 NICHHD, DEV ENDOCRINOL BRANCH, BETHESDA, MD 20892 USA. MED COLL PENN, DEPT PEDIAT, PHILADELPHIA, PA 19129 USA. RP ROSE, SR (reprint author), UNIV NEW MEXICO, MED CTR, DEPT PEDIAT, ALBUQUERQUE, NM 87131 USA. NR 50 TC 204 Z9 205 U1 2 U2 13 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD AUG PY 1991 VL 73 IS 2 BP 428 EP 435 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FY511 UT WOS:A1991FY51100033 PM 1856268 ER PT J AU BAKER, BL AXIOTIS, C HURWITZ, ES LEAVITT, R DIBISCEGLIE, AM AF BAKER, BL AXIOTIS, C HURWITZ, ES LEAVITT, R DIBISCEGLIE, AM TI NODULAR REGENERATIVE HYPERPLASIA OF THE LIVER IN IDIOPATHIC HYPEREOSINOPHILIC SYNDROME SO JOURNAL OF CLINICAL GASTROENTEROLOGY LA English DT Article DE IDIOPATHIC HYPEREOSINOPHILIC SYNDROME; NODULAR REGENERATIVE HYPERPLASIA; VASCULAR LESION AB We report a 52-year-old man with idiopathic hypereosinophilic syndrome and nodular regenerative hyperplasia of the liver. We postulate that nodular regenerative hyperplasia may be the result of an eosinophil-induced vascular lesion. C1 NIAID,BETHESDA,MD 20892. NIH,WARREN GRANT MAGNUSON CLIN CTR,OFF DIRECTOR,BETHESDA,MD 20892. RP BAKER, BL (reprint author), MIDDKD,DIGEST DIS BRANCH,LIVER DIS SECT,BLDG 10,ROOM 4D-52,BETHESDA,MD 20892, USA. NR 14 TC 5 Z9 5 U1 0 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0192-0790 J9 J CLIN GASTROENTEROL JI J. Clin. Gastroenterol. PD AUG PY 1991 VL 13 IS 4 BP 452 EP 456 PG 5 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA FX684 UT WOS:A1991FX68400017 PM 1918853 ER PT J AU MYERS, BD NELSON, RG WILLIAMS, GW BENNETT, PH HARDY, SA BERG, RL LOON, N KNOWLER, WC MITCH, WE AF MYERS, BD NELSON, RG WILLIAMS, GW BENNETT, PH HARDY, SA BERG, RL LOON, N KNOWLER, WC MITCH, WE TI GLOMERULAR FUNCTION IN PIMA-INDIANS WITH NONINSULIN-DEPENDENT DIABETES-MELLITUS OF RECENT ONSET SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE HYPERFILTRATION; BARRIER SIZE-SELECTIVITY; TRANSMEMBRANE PROTEIN SHUNTING; HETEROPOROUS MEMBRANE MODELS ID URINE SAMPLES; FILTRATION RATE; KIDNEY-FUNCTION; PROTEINURIA; NEPHROPATHY; SELECTIVITY; SIZE; MACROMOLECULES; HEMODYNAMICS; HYPERTENSION AB Differential solute clearances were used to characterize glomerular function in 20 Pima Indians with noninsulin-dependent diabetes mellitus (NIDDM) of < 3 yr duration. 28 Pima Indians with normal glucose tolerance served as controls. In the diabetic group, the glomerular filtration rate (GFR, iothalamate clearance) exceeded the control value by 15% (140 +/- 6 vs. 122 +/- 5 ml/min, P < 0.01). A corresponding 12% increase in renal plasma flow (RPF) was not statistically significant and did not account fully for the observed hyperfiltration, suggesting a concomitant elevation of the ultrafiltration pressure or coefficient. The median albumin excretion ratio in NIDDM exceeded control by almost twofold (10.1 vs. 5.8 mg/g creatinine), a trend which just failed to achieve statistical significance (P = 0.06). Fractional clearances of dextrans of broad size distribution were also elevated in diabetic subjects, significantly so for larger dextrans of between 48 and 60 angstrom radius. A theoretical analysis of dextran transport through a heteroporous membrane revealed glomerular pores in NIDDM to be uniformly shifted towards pores of larger size than in controls. We conclude that an impairment of barrier size selectivity combined with high GFR elevates the filtered protein load in NIDDM of recent onset. We propose that enhanced transglomerular trafficking of protein may predispose to sclerosis of glomeruli in those Pima Indians with NIDDM who ultimately develop diabetic nephropathy. C1 CLEVELAND CLIN FDN,CLEVELAND,OH 44195. EMORY UNIV,SCH MED,DIV RENAL,ATLANTA,GA 30322. NIDDKD,PHOENIX EPIDEMIOL & CLIN RES BRANCH,PHOENIX,AZ 85014. CLEVELAND CLIN FDN,DEPT BIOSTAT & EPIDEMIOL,PHOENIX,AZ 85016. RP MYERS, BD (reprint author), STANFORD UNIV,MED CTR,SCH MED,DIV NEPHROL,ROOM S215,STANFORD,CA 94305, USA. RI Nelson, Robert/B-1470-2012 FU NIDDK NIH HHS [N01-DK-7-2291] NR 39 TC 114 Z9 115 U1 0 U2 1 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD AUG PY 1991 VL 88 IS 2 BP 524 EP 530 DI 10.1172/JCI115335 PG 7 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA GA276 UT WOS:A1991GA27600025 PM 1864963 ER PT J AU GILL, VJ TRAVIS, LB WILLIAMS, DY AF GILL, VJ TRAVIS, LB WILLIAMS, DY TI CLINICAL AND MICROBIOLOGICAL OBSERVATIONS ON CDC GROUP DF-3, A GRAM-NEGATIVE COCCOBACILLUS SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID PATIENT AB Sequential stool cultures submitted for routine culture were screened for the presence of CDC group DF-3. Of 690 specimens, 11 (1.6%) yielded moderate to heavy growth of DF-3. Information on the 11 patients from whom these specimens were obtained showed that 4 had a history of prolonged diarrheal disease that resolved after specific therapy to eradicate DF-3, while for the other 7 patients no clear role could be established. Microbiological characterization of the stool isolates and 10 CDC strains of DF-3 suggested the presence of two subtypes within the group. Antibiotic susceptibility studies showed DF-3 to be relatively resistant to a wide variety of antibiotics. C1 NCI,DIV CANC ETIOL,EPIDEMIOL & BIOSTAT PROGRAM,BETHESDA,MD 20892. RP GILL, VJ (reprint author), WARREN GRANT MAGNUSON CLIN CTR,DEPT CLIN PATHOL,MICROBIOL SERV,BETHESDA,MD 20892, USA. NR 6 TC 5 Z9 5 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD AUG PY 1991 VL 29 IS 8 BP 1589 EP 1592 PG 4 WC Microbiology SC Microbiology GA FY046 UT WOS:A1991FY04600006 PM 1761678 ER PT J AU SHIH, JWK CHEUNG, LC ALTER, HJ LEE, LM GU, JR AF SHIH, JWK CHEUNG, LC ALTER, HJ LEE, LM GU, JR TI STRAIN ANALYSIS OF HEPATITIS-B VIRUS ON THE BASIS OF RESTRICTION ENDONUCLEASE ANALYSIS OF POLYMERASE CHAIN-REACTION PRODUCTS SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID SURFACE-ANTIGEN; ENZYMATIC AMPLIFICATION; NUCLEOTIDE-SEQUENCE; SUBTYPE ADR; DNA; HETEROGENEITY; DETERMINANTS; TRANSMISSION; HBSAG; D/Y AB To assess the value of classifying hepatitis B virus (HBV) strains at the genomic level with products from the polymerase chain reaction (PCR), an HBV PCR DNA typing procedure was developed. The design of this method was based on the selective sensitivity of the PCR product to digestion with different restriction endonucleases and on the size of the fragment resulting from a specific nuclease digestion. On the basis of published nucleotide sequences of different HBV subtypes, a set of primers was selected within the preS-S region. One of the primers was 1679F containing 25 nucleotides (5'-GGGTGGAGCCCTCAGGCTCAGGGC A-3'), and the other was 2254R containing 24 nucleotides (5'-GAAGATGAGGCATAGCAGCAGGAT-3'). All of the reactive sera produced the same sized 575-bp identification band. The product was subjected to digestion by a selected panel of restriction endonucleases. By using prototype HBV of known subtype as a model, these restriction nuclease maps of the PCR product were subtype specific. Most adr subtype clinical samples produced predicted PCR DNA restriction nuclease fragmentation in accordance with the nucleotide sequence of the prototype virus. Occasionally, samples of either the adw or the ayw subtype gave discrepant results in which they appeared to be a different subtype or were resistant to the restriction nuclease digestion. This genetic alteration was consistent in the paired specimens from sexual transmission cases. These results show that the described procedures are applicable to HBV strain assessment. C1 NCI,FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21701. SHANGHAI CANC INST,SHANGHAI,PEOPLES R CHINA. RP SHIH, JWK (reprint author), NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT TRANSFUS MED,BETHESDA,MD 20892, USA. NR 24 TC 22 Z9 22 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD AUG PY 1991 VL 29 IS 8 BP 1640 EP 1644 PG 5 WC Microbiology SC Microbiology GA FY046 UT WOS:A1991FY04600016 PM 1684796 ER PT J AU KEMKER, BJ LEHMANN, PF LEE, JW WALSH, TJ AF KEMKER, BJ LEHMANN, PF LEE, JW WALSH, TJ TI DISTINCTION OF DEEP VERSUS SUPERFICIAL CLINICAL AND NONCLINICAL ISOLATES OF TRICHOSPORON-BEIGELII BY ISOENZYMES AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS OF RDNA GENERATED BY POLYMERASE CHAIN-REACTION SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID SOLE SOURCE; URIC-ACID; YEASTS; INFECTION; PROTEIN; CARBON; ENERGY AB Fifteen clinical and environmental strains of Trichosporon beigelii were analyzed for similarities by using morphological features, biochemical profiles based on carbon compound assimilation and uric acid utilization, isoenzyme electrophoresis, and restriction fragment length polymorphisms of a segment of genes coding for rRNA expanded with the polymerase chain reaction. The findings suggest that strains that cause invasive disease are distinct from the superficial and the nonclinical isolates from the skin and mucosae represent a number of different organisms, including some environmental forms. The study shows that T. beigelii is a complex of genetically distinct organisms and that more than one type is found in clinical samples. C1 MED COLL OHIO,DEPT MICROBIOL,POB 10008,TOLEDO,OH 43699. NCI,INFECT DIS SECT,BETHESDA,MD 20892. FU NIAID NIH HHS [AI25679] NR 30 TC 40 Z9 41 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD AUG PY 1991 VL 29 IS 8 BP 1677 EP 1683 PG 7 WC Microbiology SC Microbiology GA FY046 UT WOS:A1991FY04600022 PM 1684798 ER PT J AU LONGO, DL DUFFEY, PL DEVITA, VT WIERNIK, PH HUBBARD, SM PHARES, JC BASTIAN, AW JAFFE, ES YOUNG, RC AF LONGO, DL DUFFEY, PL DEVITA, VT WIERNIK, PH HUBBARD, SM PHARES, JC BASTIAN, AW JAFFE, ES YOUNG, RC TI TREATMENT OF ADVANCED-STAGE HODGKINS-DISEASE - ALTERNATING NONCROSS-RESISTANT MOPP CABS IS NOT SUPERIOR TO MOPP SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID COMBINATION CHEMOTHERAPY; DOSE INTENSITY; FOLLOW-UP; BLEOMYCIN; LEUKEMIA; STREPTOZOTOCIN; CCNU; IV; ADRIAMYCIN; SURVIVAL C1 NCI,DIV CANC TREATMENT,BETHESDA,MD 20892. NCI,DIV CANC BIOL & DIAG & CTR,BETHESDA,MD 20892. RP LONGO, DL (reprint author), NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,BLDG 576,FREDERICK,MD 21702, USA. NR 41 TC 40 Z9 41 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD AUG PY 1991 VL 9 IS 8 BP 1409 EP 1420 PG 12 WC Oncology SC Oncology GA FY959 UT WOS:A1991FY95900014 PM 1712836 ER PT J AU STIFF, PJ MCKENZIE, RS POTEMPA, LD ALBAIN, K KOCH, D BRAUD, E BANSAL, VK WEIDNER, MK LANZOTTI, VJ CHUN, HG JOHNSON, C FISHER, RI AF STIFF, PJ MCKENZIE, RS POTEMPA, LD ALBAIN, K KOCH, D BRAUD, E BANSAL, VK WEIDNER, MK LANZOTTI, VJ CHUN, HG JOHNSON, C FISHER, RI TI A PHASE-I TRIAL OF HIGH-DOSE DIAZIQUONE AND AUTOLOGOUS BONE-MARROW TRANSPLANTATION - AN ILLINOIS-CANCER-COUNCIL-STUDY SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID ACUTE NONLYMPHOCYTIC LEUKEMIA; SYSTEM ANTITUMOR AGENTS; AZIRIDINYLBENZOQUINONE NSC-182986; BRAIN-TUMORS; AZQ; TOXICITY; LYMPHOMA; REGIMEN; INVITRO C1 LOYOLA UNIV,STRITCH SCH MED,DEPT MED,NEPHROL SECT,MAYWOOD,IL 60153. SO ILLINOIS UNIV,SCH MED,DEPT MED,HEMATOL ONCOL SECT,SPRINGFIELD,IL 62708. ILLINOIS CANC COUNCIL,CHICAGO,IL. NCI,DIV CANC TREATMENT,CANC THERAPY EVALUAT PROGRAM,INVEST DRUG BRANCH,BETHESDA,MD 20892. RP STIFF, PJ (reprint author), LOYOLA UNIV,MED CTR,STRITCH SCH MED,DEPT MED,HEMATOL ONCOL SECT,BLDG 54,2160 S 1ST AVE,MAYWOOD,IL 60153, USA. FU NCI NIH HHS [2P30-CA-21742] NR 26 TC 10 Z9 10 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD AUG PY 1991 VL 9 IS 8 BP 1487 EP 1494 PG 8 WC Oncology SC Oncology GA FY959 UT WOS:A1991FY95900025 PM 2072148 ER PT J AU CELLA, DF ORAV, EJ KORNBLITH, AB HOLLAND, JC SILBERFARB, PM LEE, KW COMIS, RL PERRY, M COOPER, R MAURER, LH HOTH, DF PERLOFF, M BLOOMFIELD, CD MCINTYRE, OR LEONE, L LESNICK, G NISSEN, N GLICKSMAN, A HENDERSON, E BARCOS, M CRICHLOW, R FAULKNER, CS EATON, W NORTH, W SCHEIN, PS CHU, F KING, G CHAHINIAN, AP AF CELLA, DF ORAV, EJ KORNBLITH, AB HOLLAND, JC SILBERFARB, PM LEE, KW COMIS, RL PERRY, M COOPER, R MAURER, LH HOTH, DF PERLOFF, M BLOOMFIELD, CD MCINTYRE, OR LEONE, L LESNICK, G NISSEN, N GLICKSMAN, A HENDERSON, E BARCOS, M CRICHLOW, R FAULKNER, CS EATON, W NORTH, W SCHEIN, PS CHU, F KING, G CHAHINIAN, AP TI SOCIOECONOMIC-STATUS AND CANCER SURVIVAL SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID LEUKEMIA GROUP-B; COMBINED MODALITY THERAPY; SMALL-CELL CARCINOMA; MULTIPLE-MYELOMA; ECONOMIC-FACTORS; CHEMOTHERAPY; BLACK; LUNG; RADIOTHERAPY; PROGNOSIS C1 HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. DARTMOUTH COLL,HITCHCOCK MED CTR,HANOVER,NH 03756. FOX CHASE CANC INST,PHILADELPHIA,PA 19111. US BIOSCI INC,BLUE BELL,PA. UNIV MISSOURI,MED CTR,SCH MED,COLUMBIA,MO 65201. WAKE FOREST UNIV,BOWMAN GRAY SCH MED,WINSTON SALEM,NC 27103. NCI,BETHESDA,MD 20892. RHODE ISL HOSP,PROVIDENCE,RI 02902. BROWN UNIV,SCH MED,PROVIDENCE,RI 02912. FINSEN INST,DK-2100 COPENHAGEN,DENMARK. CANC TREATMENT CTR,GREENVILLE,SC. ROSWELL PK CANC INST,BUFFALO,NY. ST LUKES ROOSEVELT HOSP,NEW YORK,NY 10025. MEM SLOAN KETTERING CANC CTR,NEW YORK,NY 10021. MT SINAI MED CTR,NEW YORK,NY 10029. CORNELL UNIV,MED CTR,NEW YORK HOSP,NEW YORK,NY 10021. RP CELLA, DF (reprint author), RUSH PRESBYTERIAN ST LUKES MED CTR,DEPT PSYCHOL & SOCIAL SCI,1653 W CONGRESS PKWY,CHICAGO,IL 60612, USA. NR 35 TC 139 Z9 141 U1 1 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD AUG PY 1991 VL 9 IS 8 BP 1500 EP 1509 PG 10 WC Oncology SC Oncology GA FY959 UT WOS:A1991FY95900027 PM 2072149 ER PT J AU PIGOTT, TA PATO, MT LHEUREUX, F HILL, JL GROVER, GN BERNSTEIN, SE MURPHY, DL AF PIGOTT, TA PATO, MT LHEUREUX, F HILL, JL GROVER, GN BERNSTEIN, SE MURPHY, DL TI A CONTROLLED COMPARISON OF ADJUVANT LITHIUM-CARBONATE OR THYROID-HORMONE IN CLOMIPRAMINE-TREATED PATIENTS WITH OBSESSIVE-COMPULSIVE DISORDER SO JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY LA English DT Article ID TREATMENT-REFRACTORY DEPRESSION; TRICYCLIC ANTIDEPRESSANTS; AUGMENTATION; RESISTANT; FLUVOXAMINE; EFFICACY; PLACEBO; ADJUNCT; FLUOXETINE; EXPOSURE AB In this study, 16 patients with obsessive-compulsive disorder (OCD) who had partially improved during at least 6 months of treatment with clomipramine were sequentially treated with triiodothyronine and lithium carbonate in an 8-week double-blind crossover study. Both triiodothyronine and lithium carbonate have been reported to be efficacious in open trials as adjunctive agents when combined with tricyclics in the treatment of OCD and depressed patients. However, in our controlled study, OCD and depressive symptoms, as assessed by standardized rating scales in the patient group as a whole, did not significantly change after either adjuvant treatment. Further analysis on an individual patient basis revealed that neither adjuvant medication was associated with a clinically meaningful change (> 25%) in OCD symptoms. However, lithium, but not triiodothyronine, adjuvant therapy was associated with a 25% or greater reduction in depression scores in 44% of the patients. This controlled study lends further support to the contention that OCD may represent a disorder with characteristics distinct from affective disorders. RP PIGOTT, TA (reprint author), NIMH,CTR RADIOL,CLIN SCI LAB,10-3D41,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 51 TC 97 Z9 98 U1 1 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0271-0749 J9 J CLIN PSYCHOPHARM JI J. Clin. Psychopharmacol. PD AUG PY 1991 VL 11 IS 4 BP 242 EP 248 PG 7 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA FY157 UT WOS:A1991FY15700004 PM 1918422 ER PT J AU ARAI, R WINSKY, L ARAI, M JACOBOWITZ, DM AF ARAI, R WINSKY, L ARAI, M JACOBOWITZ, DM TI IMMUNOHISTOCHEMICAL LOCALIZATION OF CALRETININ IN THE RAT HINDBRAIN SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE CALCIUM-BINDING PROTEIN; STREPTAVIDIN-PEROXIDASE CONJUGATE; BRAIN MAPPING; SUBSTANTIA NIGRA; CEREBELLUM ID CALCIUM-BINDING PROTEIN; NERVOUS-SYSTEM; NEURONS; NUCLEUS; IMMUNOREACTIVITY; ORGANIZATION; PARVALBUMIN; CEREBELLUM; CALBINDIN; FOREBRAIN AB The localization of calretinin in the rat hindbrain was examined immunohistochemically with an antiserum against calretinin purified from the guinea pig brain. Calretinin immunoreactivity was found within neuronal elements. The distribution of calretinin-immunoreactive cell bodies and fibers is presented in schematic drawings and summarized in a table. Major calretinin-immunoreactive neurons were found in the lateral and medial geniculate nuclei, substantia nigra, ventral tegmental area, interpeduncular nucleus, periaqueductal gray, mesencephalic trigeminal nucleus, superior and inferior colliculi, pontine nuclei, parabrachial nucleus, dorsal and laterodorsal tegmental nuclei, cochlear nuclei, vestibular nuclei, medullary reticular nuclei, nucleus of the solitary tract, area postrema, substantia gelatinosa of the spinal trigeminal nucleus, and cerebellum. These results show that distinct calretinin-immunoreactive neurons are widely distributed in the rat hindbrain. C1 NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NR 40 TC 184 Z9 184 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD AUG 1 PY 1991 VL 310 IS 1 BP 21 EP 44 DI 10.1002/cne.903100105 PG 24 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA GA103 UT WOS:A1991GA10300003 PM 1939729 ER PT J AU LAZAR, GY LIPOSITS, Z TOTH, P TRASTI, SL MADERDRUT, JL MERCHENTHALER, I AF LAZAR, GY LIPOSITS, Z TOTH, P TRASTI, SL MADERDRUT, JL MERCHENTHALER, I TI DISTRIBUTION OF GALANIN-LIKE IMMUNOREACTIVITY IN THE BRAIN OF RANA-ESCULENTA AND XENOPUS-LAEVIS SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE EVOLUTION; FROG; IMMUNOCYTOCHEMISTRY; NEUROANATOMY; NEUROENDOCRINOLOGY; NEUROPEPTIDES; OPTIC TECTUM; VISION ID CENTRAL NERVOUS-SYSTEM; HYPOTHALAMO-NEUROHYPOPHYSEAL SYSTEM; PROENKEPHALIN-DERIVED PEPTIDES; NUCLEOTIDE-SEQUENCE ANALYSIS; HORMONE-RELEASING HORMONE; BASAL FOREBRAIN COMPLEX; P-LIKE IMMUNOREACTIVITY; GENE-RELATED PEPTIDE; ANURAN OPTIC TECTUM; SUBSTANCE-P AB The immunocytochemical distribution of galanin-containing perikarya and nerve terminals in the brain of Rana esculenta and Xenopus laevis was determined with antisera directed toward either porcine or rat galanin. The pattern of galanin-like immunoreactivity appeared to be identical with antisera directed toward either target antigen. The distribution of galanin-like immunoreactivity was similar in Rana esculenta and Xenopus laevis except for the absence of a distinct laminar distribution of immunoreactivity in the optic tectum of Xenopus laevis. Galanin-containing perikarya were located in all major subdivisions of the brain except the metencephalon. In the telencephalon, immunoreactive perikarya were detected in the pars medialis of the amygdala and the preoptic area. In the diencephalon, immunoreactive perikarya were detected in the caudal half of the suprachiasmatic nucleus, the nucleus of the periventricular organ, the ventral hypothalamus, and the median eminence. In the mesencephalon, immunoreactive perikarya were detected near the midline of the rostroventral tegmentum, in the torus semicircularis and, occassionally, in lamina A and layer 6 of the optic tectum. In the myelencephalon, labelled perikarya were detected only in the caudal half of the nucleus of the solitary tract. Immunoreactive nerve fibers of varying density were observed in all subdivisions of the brain with the densest accumulations of fibers occurring in the pars lateralis of the amygdala and the preoptic area. Dense accumulations of nerve fibers were also found in the lateral septum, the medial forebrain bundle, the periventricular region of the diencephalon, the ventral hypothalamus, the median eminence, the mesencephalic central gray, the laminar nucleus of the torus semicircularis, several laminae of the optic tectum, the interpeduncular nucleus, the isthmic nucleus, the central gray of the rhombencephalon, and the dorsolateral caudal medulla. The extensive system of galanin-containing perikarya and nerve fibers in the brain of representatives of two families of anurans showed many similarities to the distribution of galanin-containing perikarya and nerve fibers previously described for the mammalian brain. C1 NIEHS, MOLEC & INTEGRAT NEUROSCI LAB, FUNCT MORPHOL SECT, MD C4-07, RES TRIANGLE PK, NC 27709 USA. UNIV PECS, SCH MED, DEPT ANAT, H-7643 PECS, HUNGARY. NR 100 TC 59 Z9 59 U1 0 U2 1 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD AUG 1 PY 1991 VL 310 IS 1 BP 45 EP 67 DI 10.1002/cne.903100106 PG 23 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA GA103 UT WOS:A1991GA10300004 PM 1719037 ER PT J AU LUPER, WD EICHMILLER, FC DOBLECKI, W CAMPBELL, D LI, SH AF LUPER, WD EICHMILLER, FC DOBLECKI, W CAMPBELL, D LI, SH TI EFFECT OF 3 STERILIZATION TECHNIQUES ON FINGER PLUGGERS SO JOURNAL OF ENDODONTICS LA English DT Article ID ENDODONTIC INSTRUMENTS; CUTTING EFFICIENCY; FILES AB The effects of different sterilization methods on the fatigue life of finger pluggers were investigated. Ninety finger pluggers for each of four sizes (A, B, C, and D) were subdivided into subgroups of 10. Each subgroup was subjected to 1, 8, or 15 cycles of steam autoclave, dry heat, or bead sterilization. Ten control pluggers for each size were not sterilized. After sterilization, experimental and control finger pluggers were subjected to cyclic bending until fracture. Only the A finger pluggers autoclaved for eight cycles had a significantly lower number of cycles to failure compared with that of the controls. Nine subgroups had significantly greater number of cycles before failure than did the control. Because all but one sterilized group had fatigue lifetimes statistically equal to or greater than nonsterilized controls, clinicians generally can use any of the three sterilization methods without fear of plugger failure. C1 USN,DEPT ENDODONT,DENT CLIN,BREMERTON,WA. NATL INST STAND & TECHNOL,AMER DENT ASSOC HLTH FDN,GAITHERSBURG,MD. FMFPAC,1ST DENT BN,EL TORO,CA. USN,SCH DENT,DEPT ENDODONT,BETHESDA,MD 20814. NIDR,BIOMETRY SECT,BETHESDA,MD 20892. FU PHS HHS [M00095-06-3014] NR 11 TC 2 Z9 4 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0099-2399 J9 J ENDODONT JI J. Endod. PD AUG PY 1991 VL 17 IS 8 BP 361 EP 364 PG 4 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA FZ185 UT WOS:A1991FZ18500001 PM 1809797 ER PT J AU CISAR, JO BARSUMIAN, EL SIRAGANIAN, RP CLARK, WB YEUNG, MK HSU, SD CURL, SH VATTER, AE SANDBERG, AL AF CISAR, JO BARSUMIAN, EL SIRAGANIAN, RP CLARK, WB YEUNG, MK HSU, SD CURL, SH VATTER, AE SANDBERG, AL TI IMMUNOCHEMICAL AND FUNCTIONAL-STUDIES OF ACTINOMYCES-VISCOSUS T14V TYPE-1 FIMBRIAE WITH MONOCLONAL AND POLYCLONAL ANTIBODIES DIRECTED AGAINST THE FIMBRIAL SUBUNIT SO JOURNAL OF GENERAL MICROBIOLOGY LA English DT Article ID SALIVA-TREATED HYDROXYAPATITE; ESCHERICHIA-COLI; APATITIC SURFACES; GENE-PRODUCTS; ADSORPTION; EXPRESSION; PROTEINS; IDENTIFICATION; NAESLUNDII; RECEPTORS AB Each of five monoclonal antibodies (mAbs) prepared against the type 1 fimbriae of Actinomyces viscosus T14V reacted with a 54 kDa cloned protein previously identified as a fimbrial subunit. This purified protein completely inhibited the reaction of a specific anti-type-1-fimbria rabbit antibody with A. viscosus whole cells. Maximum values for the number of antibody molecules bound per bacterial cell ranged from 7 x 10(3) to 1.2 x 10(4) for the different I-125-labelled mAbs and was approximately 7 x 10(4) for I-125-labelled rabbit IgG or Fab against either type 1 fimbriae or the 54 kDa cloned protein. Although the different mAbs, either individually or as a mixture, failed to inhibit the type-1-fimbria-mediated adherence of A. viscosus T14V to saliva-treated hydroxyapatite, each rabbit antibody gave 50% inhibition of adherence when approximately 5 x 10(4) molecules of IgG were bound per cell. However, binding of each corresponding rabbit Fab had no significant effect on bacterial attachment unless much higher concentrations were used. These findings suggest that antibodies directed solely against the 54 kDa fimbrial subunit do not react with the putative receptor binding sites of A. viscosus T14V type 1 fimbriae. Instead, inhibition of attachment by the polyclonal antibodies may depend on an indirect effect of antibody binding that prevents the fimbria-receptor interaction. C1 NIDR,IMMUNOL LAB,BETHESDA,MD 20892. UNIV FLORIDA,DEPT ORAL BIOL,GAINESVILLE,FL 32610. UNIV COLORADO,MED CTR,WEBB WARING LUNG INST,DENVER,CO 80262. RP CISAR, JO (reprint author), NIDR,MICROBIAL ECOL LAB,BETHESDA,MD 20892, USA. NR 30 TC 26 Z9 26 U1 0 U2 0 PU SOC GENERAL MICROBIOLOGY PI READING PA HARVEST HOUSE 62 LONDON ROAD, READING, BERKS, ENGLAND RG1 5AS SN 0022-1287 J9 J GEN MICROBIOL JI J. Gen. Microbiol. PD AUG PY 1991 VL 137 BP 1971 EP 1979 PN 8 PG 9 WC Microbiology SC Microbiology GA GB638 UT WOS:A1991GB63800018 PM 1683401 ER PT J AU ABE, R KANAGAWA, O SHEARD, MA MALISSEN, B FOOPHILLIPS, M AF ABE, R KANAGAWA, O SHEARD, MA MALISSEN, B FOOPHILLIPS, M TI CHARACTERIZATION OF A NEW MINOR LYMPHOCYTE STIMULATORY SYSTEM .1. CLUSTER OF SELF ANTIGENS RECOGNIZED BY I-E-REACTIVE V-BETA-S, V-BETA-5, V-BETA-11, AND V-BETA-12 T-CELL RECEPTORS FOR ANTIGEN SO JOURNAL OF IMMUNOLOGY LA English DT Article ID MAJOR HISTOCOMPATIBILITY COMPLEX; GENE-PRODUCTS; IMPARTS REACTIVITY; CLONAL DELETION; MLS; TOLERANCE; EXPRESSION; DETERMINANT; ANTIBODY; INVIVO AB In the mouse, two sets of V-beta gene products have been shown to be associated with T cell recognition of endogenous self Ag. One of these is the set of V-beta associated with T cell reactivities to stimulatory Mls gene products, Mls(a) (V-beta-6, V-beta-8.1, V-beta-9) or Mls(c) (V-beta-3); another is the set of V-beta, such as V-beta-5, V-beta-11, V-beta-12, or V-beta-17a, which were originally found to be related to I-E recognition. Although the Mls system has been well characterized, little is known about the nature of the ligands for the second set of V-beta. In this work, we describe the evidence that the natural ligand or ligands of V-beta-5, V-beta-11, and V-beta-12 may be novel Mls determinants that are recognized by naive T cells at a high precursor frequency and function as the ligand for clonal deletion of self-reactive T cells by negative selection. However, surprisingly, unlike the conventional Mls system, in which all V-beta associated with Mls(a) recognition or Mls(c) recognition are uniformly deleted in those animals expressing the relevant Mls type, expression of these three V-beta segregates independently among strains. Based on these observations, the nature of T cell recognition for this new Mls gene product(s) is discussed. C1 WASHINGTON UNIV,SCH MED,DEPT PATHOL,ST LOUIS,MO 63110. CTR IMMUNOL,CNRS MARSEILLE LUMINY,INSERM,F-13288 MARSEILLE 9,FRANCE. RP ABE, R (reprint author), NCI,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4B17,BETHESDA,MD 20892, USA. RI Ain, Kenneth/A-5179-2012 OI Ain, Kenneth/0000-0002-2668-934X FU NIAID NIH HHS [AI30803] NR 39 TC 27 Z9 27 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD AUG 1 PY 1991 VL 147 IS 3 BP 739 EP 749 PG 11 WC Immunology SC Immunology GA FY292 UT WOS:A1991FY29200001 PM 1713604 ER PT J AU SEDER, RA PLAUT, M BARBIERI, S URBAN, J FINKELMAN, FD PAUL, WE AF SEDER, RA PLAUT, M BARBIERI, S URBAN, J FINKELMAN, FD PAUL, WE TI PURIFIED FC-EPSILON-R+ BONE-MARROW AND SPLENIC NON-B, NON-T CELLS ARE HIGHLY ENRICHED IN THE CAPACITY TO PRODUCE IL-4 IN RESPONSE TO IMMOBILIZED IGE, IGG2A, OR IONOMYCIN SO JOURNAL OF IMMUNOLOGY LA English DT Article ID STIMULATORY FACTOR-I; MONOCLONAL-ANTIBODY; MAST-CELLS; SURFACE-IMMUNOGLOBULIN; INTERLEUKIN-4; RECEPTOR; IDENTIFICATION; ANTIGEN; GROWTH; INVIVO AB Non-B, non-T cells from spleen and bone marrow cells produce IL-4 in response to cross-linkage of high affinity receptors for Fc-epsilon-R or Fc-gamma-RII, and to treatment with calcium ionophores. Cells bearing high affinity Fc-epsilon-R constituted 1 to 2% of non-B, non-T cells of spleen and of total bone marrow cells from naive donors. In mice whose immune systems had been polyclonally activated by injection with anti-IgD antibodies or had been infected with Nippostrongylus brasiliensis larvae, the frequency of Fc-epsilon-R+ cells in splenic non-B, non-T cells was also 1 to 2% but in bone marrow from anti-IgD-injected mice donors the frequency was approximately 5%. Cell sorting experiments revealed that all of the capacity to produce IL-4 in response to immobilized IgE or IgG2a or to ionomycin was found in the Fc-epsilon-R+ fraction. Among the Fc-epsilon-R+ spleen cells from naive donors, the frequency of IL-4-producing cells was 1/20 to 1/40 whereas in mice that had been injected with anti-IgD or infected with N. brasiliensis, the frequency of IL-4 producing cells in the Fc-epsilon-R+ population was approximately 1/5. C1 NIAID,IMMUNOL LAB,BLDG 10,ROOM 11N311,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,SCH MED,JOHNS HOPKINS ASTHMA & ALLERGY CTR,BALTIMORE,MD 21224. UNIFORMED SERV UNIV HLTH SCI,F EDWARD HEBERT SCH MED,DEPT MED,BETHESDA,MD 20814. NIAID,BIOL RESOURCES BRANCH,BETHESDA,MD 20892. USDA ARS,BELTSVILLE AGR RES CTR,INST LIVESTOCK & POULTRY SCI,HELMINTH DIS LAB,BELTSVILLE,MD 20705. FU NIAID NIH HHS [AI 21328, AI 27906] NR 34 TC 58 Z9 58 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD AUG 1 PY 1991 VL 147 IS 3 BP 903 EP 909 PG 7 WC Immunology SC Immunology GA FY292 UT WOS:A1991FY29200023 PM 1830602 ER PT J AU UNGAR, BLP KAO, TC BURRIS, JA FINKELMAN, FD AF UNGAR, BLP KAO, TC BURRIS, JA FINKELMAN, FD TI CRYPTOSPORIDIUM INFECTION IN AN ADULT-MOUSE MODEL - INDEPENDENT ROLES FOR IFN-GAMMA AND CD4+ LYMPHOCYTES-T IN PROTECTIVE IMMUNITY SO JOURNAL OF IMMUNOLOGY LA English DT Article ID GIARDIA-MURIS INFECTION; MONOCLONAL-ANTIBODY; INTERFERON-GAMMA; NUDE-MICE; CELL RESPONSES; L3T4+; IDENTIFICATION; PROLIFERATION; INTERLEUKIN-2; LYMPHOKINE AB Cryptosporidium is a protozoan parasite that can cause chronic life-threatening diarrhea in immunocompromised persons. Host immune responses are poorly understood. an impediment to development of effective therapy. In mice, normal adult BALB/c animals resist infection whereas chronic symptomatic cryptosporidiosis develops in adult nude mice and in neonatally infected BALB/c mice treated with anti-CD4 mAb. To define further the immune defects that allow mice to be infected with Cryptosporidium, adult BALB/c mice were treated with cytolytic anti-CD4 or anti-CDS or with neutralizing anti-IFN-gamma or anti-IL-2 mAb. Chronic infection, manifested by continuous shedding of sparse but statistically significant numbers of oocysts, occurred with anti-CD4 +/- anti-CD8 mAb treatment although anti-CD8 mAb treatment alone did not allow infection. Treatment with anti-IFN-gamma mAb greatly enhanced oocyst shedding but infection was self-limited. Treatment with a combination of anti-CD4 and anti-IFN-gamma mAb permitted both chronic infection and shedding of large numbers of oocysts. Furthermore mice treated initially with anti-CD4 mAb showed a substantial increase in oocyst shedding when later treated with anti-IFN-gamma mAb; and mice treated initially with both mAbs showed a decline in oocyst shedding when anti-IFN-gamma was stopped. Anti-IFN-gamma mAb treatment of congenitally athymic adult BALB/c mice led to an approximately a 75-fold increase in oocyst shedding. Treatment of adult BALB/c mice with anti-IL-2 mAb did not permit Cryptosporidium infection. These results suggest that redundant immunologic mechanisms limit Cryptosporidium infection such that both CD4+ cells and IFN-gamma are required to prevent initiation of infection whereas either alone can limit the extent (IFN-gamma) or duration (CD4+ T cells) of infection. They also suggest that production of IFN-gamma by a non-T cell contributes to host immunity. C1 NIH,NATL CTR RES RESOURCES,DEPT COMPARAT PATHOL,BETHESDA,MD 20892. UNIFORMED SERV UNIV HLTH SCI,DEPT MED,BETHESDA,MD 20814. UNIFORMED SERV UNIV HLTH SCI,DEPT PREVENT MED,BETHESDA,MD 20814. NR 43 TC 187 Z9 193 U1 1 U2 4 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD AUG 1 PY 1991 VL 147 IS 3 BP 1014 EP 1022 PG 9 WC Immunology SC Immunology GA FY292 UT WOS:A1991FY29200039 PM 1677668 ER PT J AU WEILHILLMAN, G SCHELL, K SEGAL, DM HANK, JA SOSMAN, JA SONDEL, PM AF WEILHILLMAN, G SCHELL, K SEGAL, DM HANK, JA SOSMAN, JA SONDEL, PM TI ACTIVATION OF HUMAN T-CELLS OBTAINED PRE-INTERLEUKIN-2 AND POST-INTERLEUKIN-2 (IL-2) THERAPY BY ANTI-CD3 MONOCLONAL-ANTIBODY PLUS IL-2 - IMPLICATIONS FOR COMBINED INVIVO TREATMENT SO JOURNAL OF IMMUNOTHERAPY LA English DT Article DE ACTIVATED T-CELLS; INTERLEUKIN-2; ANTI-CD3 MONOCLONAL ANTIBODY; NATURAL KILLER CELLS; LYMPHOKINE-ACTIVATED KILLER CELLS; PERIPHERAL BLOOD LYMPHOCYTES ID TUMOR-INFILTRATING LYMPHOCYTES; REPETITIVE WEEKLY CYCLES; PERIPHERAL-BLOOD LYMPHOCYTES; NATURAL-KILLER-CELLS; RECOMBINANT INTERLEUKIN-2; CANCER-PATIENTS; METASTATIC MELANOMA; ANTITUMOR-ACTIVITY; MONONUCLEAR-CELLS; GENERATED INVIVO AB The role of activated T cells in the mediation of antitumor responses has been documented in several experimental models. In some of these, interleukin-2 (IL-2) has been used as a means to induce and expand the antitumor effects of the T cells. IL-2 has been tested in clinical trials for cancer treatment. Surprisingly, T cells appear to be inactivated by IL-2 in these clinical trials. T cells obtained from peripheral blood after IL-2 therapy showed decreased responses to mitogens and alloantigens, did not proliferate in vitro in response to IL-2, and did not mediate non-major histocompatibility complex-restricted cytotoxicity or targeted lysis in the presence of bispecific monoclonal antibodies. In this study, we present evidence that these post-IL-2 therapy T cells are not irreversibly inactivated; they can be activated in vitro by anti-CD3 monoclonal antibody together with IL-2 to upregulate the p55 component of the IL-2 receptor and proliferate. Nevertheless, following activation by anti-CD3 and IL-2, the level of targeted T-cell cytotoxicity mediated by the post-IL-2 therapy T cells was significantly lower than that by pre-IL-2 therapy T cells. Although in vivo treatment with IL-2 alone induces natural killer (NK) cells to mediate lymphokine-activated killer activity, these data suggest that the T-cell lytic function is inhibited by this treatment and only partially reversible by subsequent T-cell receptor activation using anti-CD3 mAb. Exposure of T cells to anti-CD3 mAb prior to in vivo IL-2 treatment generates T-cell lytic activity in vitro. These results, together with preclinical murine studies, suggest that a combined in vivo protocol of anti-CD3 mAb and IL-2, starting first with the anti-CD3 mAb, may cause activation of the T cells in addition to the activation of NK cells and thus warrant clinical testing. C1 UNIV WISCONSIN,DEPT HUMAN ONCOL,MADISON,WI 53792. UNIV WISCONSIN,DEPT PEDIAT,MADISON,WI 53792. UNIV WISCONSIN,DEPT GENET,MADISON,WI 53792. NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. LOYOLA UNIV,DEPT HEMATOL & ONCOL,MAYWOOD,IL 60153. FU NCI NIH HHS [CM-47669-06, CA-32685]; NCRR NIH HHS [RR-03186] NR 42 TC 22 Z9 22 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1053-8550 J9 J IMMUNOTHER JI J. Immunother. PD AUG PY 1991 VL 10 IS 4 BP 267 EP 277 DI 10.1097/00002371-199108000-00005 PG 11 WC Oncology; Immunology; Medicine, Research & Experimental SC Oncology; Immunology; Research & Experimental Medicine GA FY372 UT WOS:A1991FY37200005 PM 1834166 ER PT J AU GALLO, RC AF GALLO, RC TI HUMAN RETROVIRUSES - A DECADE OF DISCOVERY AND LINK WITH HUMAN-DISEASE SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID LONG-TERM CULTURE; KAPOSIS-SARCOMA; TAT PROTEIN; CELLS; GROWTH; HIV-1; AIDS; INHIBITION; VIRUS RP GALLO, RC (reprint author), NCI,TUMOR CELL BIOL LAB,BLDG 37,ROOM 6A09,BETHESDA,MD 20892, USA. NR 20 TC 32 Z9 32 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD AUG PY 1991 VL 164 IS 2 BP 235 EP 243 PG 9 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA FY100 UT WOS:A1991FY10000001 PM 1677372 ER PT J AU LAUGHON, BE ALLAUDEEN, HS BECKER, JM CURRENT, WL FEINBERG, J FRENKEL, JK HAFNER, R HUGHES, WT LAUGHLIN, CA MEYERS, JD SCHRAGER, LK YOUNG, LS AF LAUGHON, BE ALLAUDEEN, HS BECKER, JM CURRENT, WL FEINBERG, J FRENKEL, JK HAFNER, R HUGHES, WT LAUGHLIN, CA MEYERS, JD SCHRAGER, LK YOUNG, LS TI SUMMARY OF THE WORKSHOP ON FUTURE-DIRECTIONS IN DISCOVERY AND DEVELOPMENT OF THERAPEUTIC AGENTS FOR OPPORTUNISTIC INFECTIONS ASSOCIATED WITH AIDS SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article C1 NIAID, DIV MICROBIOL & INFECT DIS, ANTIVIRAL RES BRANCH, BETHESDA, MD 20892 USA. UNIV TENNESSEE, DEPT MICROBIOL, KNOXVILLE, TN 37996 USA. ST JUDE CHILDRENS RES HOSP, DEPT INFECT DIS, MEMPHIS, TN 38101 USA. ELI LILLY & CO, LILLY RES LAB, INDIANAPOLIS, IN 46285 USA. UNIV KANSAS, MED CTR, DEPT PATHOL, KANSAS CITY, KS 66103 USA. FRED HUTCHINSON CANC RES CTR, DEPT INFECT DIS, SEATTLE, WA 98104 USA. KUZELL INST ARTHRITIS & INFECT DIS, SAN FRANCISCO, CA USA. RP LAUGHON, BE (reprint author), NIAID, DIV AIDS, EPIDEMIOL BRANCH, TREATMENT RES PROGRAM, MED BRANCH, BETHESDA, MD 20892 USA. NR 0 TC 38 Z9 38 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD AUG PY 1991 VL 164 IS 2 BP 244 EP 251 PG 8 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA FY100 UT WOS:A1991FY10000002 PM 1649870 ER PT J AU WRIGHT, PF KING, J ARAKI, K KONDO, Y THOMPSON, J TOLLEFSON, SJ KOBAYASHI, M KAPIKIAN, AZ AF WRIGHT, PF KING, J ARAKI, K KONDO, Y THOMPSON, J TOLLEFSON, SJ KOBAYASHI, M KAPIKIAN, AZ TI SIMULTANEOUS ADMINISTRATION OF 2 HUMAN-RHESUS ROTAVIRUS REASSORTANT STRAINS OF VP7 SEROTYPE-1 AND SEROTYPE-2 SPECIFICITY TO INFANTS AND YOUNG-CHILDREN SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID VENEZUELAN INFANTS; VACCINE; DIARRHEA; PROTECTION; TRIAL AB Two rotavirus vaccine strains representing VP7 serotypes 1 and 2 derived by reassortment between a rhesus rotavirus master strain, MMU18006, and either of two human rotavirus strains were administered simultaneously to infants and young children to assess potential interactions between strains. Children were observed in a day care setting for 10 days after vaccine administration for clinical symptoms, evidence of vaccine transmission, and patterns of viral shedding. Serum and local antibody responses were measured. The ratio of input virus strongly influenced the amount of each strain recovered from the child. Regardless of dose of virus administered, the neutralizing antibody response to the VP7 glycoprotein, the serotype determinant, was diminished in a bivalent preparation compared with a monovalent vaccine. Additional strategies must be sought to induce immunity against the multiple serotypes of human rotavirus before broad immunity will be established. C1 NIAID,INFECT DIS LAB,BETHESDA,MD 20892. RP WRIGHT, PF (reprint author), VANDERBILT UNIV,DEPT PEDIAT,NASHVILLE,TN 37232, USA. FU NCRR NIH HHS [RR-00095]; NIAID NIH HHS [AI-05050] NR 17 TC 17 Z9 17 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD AUG PY 1991 VL 164 IS 2 BP 271 EP 276 PG 6 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA FY100 UT WOS:A1991FY10000006 PM 1649874 ER PT J AU MARKOFF, LJ INNIS, BL HOUGHTEN, R HENCHAL, LS AF MARKOFF, LJ INNIS, BL HOUGHTEN, R HENCHAL, LS TI DEVELOPMENT OF CROSS-REACTIVE ANTIBODIES TO PLASMINOGEN DURING THE IMMUNE-RESPONSE TO DENGUE VIRUS-INFECTION SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID PARTIAL NUCLEOTIDE-SEQUENCE; JAPANESE ENCEPHALITIS-VIRUS; YELLOW-FEVER VIRUS; STRUCTURAL PROTEINS; ACTIVATOR GENE; GENOME; ACID; RNA; CHAIN AB The four serotypes of dengue virus (a mosquito-borne flavivirus) cause an acute febrile illness (dengue fever) or a more prolonged illness with plasma leakage resulting in hypovolemia (dengue hemorrhagic fever). Hemorrhage may accompany either. Epidemiologic data suggest a role for dengue antibodies in pathogenesis. Computer analysis revealed a 20-residue region of similarity in amino acid sequence between the dengue type 4 envelope glycoprotein (E) and a family of clotting factors, including plasminogen, the prime mediator of fibrinolysis. By use of synthetic peptides in ELISA, E antibodies that potentially bind plasminogen were detected in 75% of 40 Thai patients acutely infected with dengue virus type 1, 2, 3, or 4. Plasminogen cross-reactivity of dengue antibodies was shown to be specific for the related sites in E and plasminogen. The dengue E sequence with similarity to plasminogen is largely conserved within the currently known flavivirus E sequences. However, 15 Thai patients hospitalized for illness caused by Japanese encephalitis virus (a flavivirus not associated with hemorrhage) did not develop plasminogen-cross-reactive antibodies, and this finding correlated with failure of Japanese encephalitis virus antibodies to bind to the plasminogen-cross-reactive site in E. C1 ARMED FORCES RES INST MED SCI,DEPT VIROL,BANGKOK,THAILAND. TORREY PINES INST MOLEC STUDIES,SAN DIEGO,CA. RP MARKOFF, LJ (reprint author), NIAID,INFECT DIS LAB,BLDG 7,ROOM 230,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 45 TC 44 Z9 49 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD AUG PY 1991 VL 164 IS 2 BP 294 EP 301 PG 8 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA FY100 UT WOS:A1991FY10000010 PM 1856477 ER PT J AU LIPKA, JJ SANTIAGO, P CHAN, L REYES, GR SAMUEL, KP BLATTNER, WA SHAW, GM HANSON, CV SNINSKY, JJ FOUNG, SKH AF LIPKA, JJ SANTIAGO, P CHAN, L REYES, GR SAMUEL, KP BLATTNER, WA SHAW, GM HANSON, CV SNINSKY, JJ FOUNG, SKH TI MODIFIED WESTERN-BLOT ASSAY FOR CONFIRMATION AND DIFFERENTIATION OF HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-I AND TYPE-II SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID HTLV-II; PROTEIN; LEUKEMIA; PATIENT AB Disease association studies of human T cell lymphotropic virus (HTLV) types I and II are hindered by the need for multiple assays to confirm and differentiate between the viruses. A modified Western blot assay has been developed using HTLV-I viral lysate and unique (MTA-4) and shared (p21E) HTLV recombinant proteins. By defining confirmation of infection as the presence of antibodies to p24 gag protein and to p21E, all 56 HTLV-I and 49 HTLV-II antisera were confirmed by this modified Western blot alone. Differentiation was determined by reactivity to MTA-4. All HTLV-I antisera reacted with MTA-4 and all HTLV-II antisera did not react with MTA-4. These findings indicate the utility of selected HTLV-I recombinant proteins in a single assay format to confirm and differentiate infections with HTLV-I and HTLV-II. C1 STANFORD UNIV,MED CTR,SCH MED,DEPT PATHOL,BLOOD BANK,800 WELCH RD,STANFORD,CA 94305. GENELABS INC,DEPT MOLEC VIROL,REDWOOD CITY,CA. CALIF DEPT HLTH SERV,VIRAL & RICKETTSIAL DIS LAB,BERKELEY,CA. CETUS CORP,EMERYVILLE,CA 94608. FREDERICK CANC RES & DEV CTR,CELLULAR BIOCHEM LAB,BIOTECHNOL WORKING GRP,FREDERICK,MD. NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. UNIV ALABAMA,DEPT MED,BIRMINGHAM,AL 35294. FU NHLBI NIH HHS [HL-33811]; NIDA NIH HHS [DA-06596] NR 14 TC 33 Z9 33 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD AUG PY 1991 VL 164 IS 2 BP 400 EP 403 PG 4 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA FY100 UT WOS:A1991FY10000025 PM 1856487 ER PT J AU KAN, VL BENNETT, JE AMANTEA, MA SMOLSKIS, MC MCMANUS, E GRASELA, DM SHERMAN, JW AF KAN, VL BENNETT, JE AMANTEA, MA SMOLSKIS, MC MCMANUS, E GRASELA, DM SHERMAN, JW TI COMPARATIVE SAFETY, TOLERANCE, AND PHARMACOKINETICS OF AMPHOTERICIN-B LIPID COMPLEX AND AMPHOTERICIN-B DEOXYCHOLATE IN HEALTHY MALE-VOLUNTEERS SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID FUNGAL-INFECTIONS; CANCER-PATIENTS; TOXICITY AB Amphotericin B lipid complex (ABLC), a lipid complex formulation of amphotericin B, and amphotericin B desoxycholate (AB) were compared for safety, tolerance, and pharmacokinetics in two groups of eight healthy male volunteers. After a 1-mg test dose, study drug was infused at 0.1, 0.25, and 0.5 mg/kg; the 0.5-mg/kg dose was not given to subjects receiving AB. ABLC caused few acute adverse effects except for mild somnolence (drowsiness) in six volunteers. In addition, three of eight ABLC recipients had asymptomatic, transient serum transaminase elevations that resolved spontaneously. The AB recipients experienced more acute side effects, but only one had a mild shaking chill; three of eight also experienced sleepiness. No significant changes in vital signs, electrocardiograms, oximetry, pulmonary function, or clinical status were observed in either group. Due to its increased estimated volume of distribution and estimated clearance, ABLC yielded decreased amphotericin B levels and area under the serum concentration versus time curve relative to AB. C1 NIH,CTR CLIN,DEPT PHARM,BETHESDA,MD 20892. NIH,CTR CLIN,DEPT NURSING,BETHESDA,MD 20892. BRISTOL MYERS SQUIBB PHARMACEUTICAL RES INST,PRINCETON,NJ. RP KAN, VL (reprint author), NIAID,CLIN MYCOL SECT,BLDG 10,RM 11N107,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 10 TC 85 Z9 88 U1 0 U2 2 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD AUG PY 1991 VL 164 IS 2 BP 418 EP 421 PG 4 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA FY100 UT WOS:A1991FY10000030 PM 1856491 ER PT J AU BLEECKER, ER MCFADDEN, ER BOUSHEY, HA EDELL, ES ESCHENBACHER, WL GODARD, PP GOLDSTEIN, RA HOLGATE, ST HUNNINGHAKE, GW HURD, SS LAITINEN, A LICHTENSTEIN, LM PROGRAIS, L RANKIN, JA RAM, JS REED, CE REYNOLDS, HY WOOD, RE AF BLEECKER, ER MCFADDEN, ER BOUSHEY, HA EDELL, ES ESCHENBACHER, WL GODARD, PP GOLDSTEIN, RA HOLGATE, ST HUNNINGHAKE, GW HURD, SS LAITINEN, A LICHTENSTEIN, LM PROGRAIS, L RANKIN, JA RAM, JS REED, CE REYNOLDS, HY WOOD, RE TI INVESTIGATIVE USE OF BRONCHOSCOPY, LAVAGE AND BRONCHIAL BIOPSIES IN ASTHMA AND OTHER AIRWAYS DISEASES SO JOURNAL OF INVESTIGATIONAL ALLERGOLOGY & CLINICAL IMMUNOLOGY LA English DT Article C1 NHLBI,WESTWOOD BLDG,ROOM 6A-15,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU J R PROUS SA PI BARCELONA PA APARTADO DE CORREOS 540, PROVENZA 388, 08025 BARCELONA, SPAIN SN 1018-9068 J9 J INVEST ALLERG CLIN JI J. Invest. Allergol. Clin. Immunol. PD AUG PY 1991 VL 1 IS 4 BP 271 EP 277 PG 7 WC Allergy; Immunology SC Allergy; Immunology GA GM470 UT WOS:A1991GM47000008 ER PT J AU SWERLICK, RA GARCIAGONZALEZ, E KUBOTA, Y XU, YL LAWLEY, TJ AF SWERLICK, RA GARCIAGONZALEZ, E KUBOTA, Y XU, YL LAWLEY, TJ TI STUDIES OF THE MODULATION OF MHC ANTIGEN AND CELL-ADHESION MOLECULE EXPRESSION ON HUMAN DERMAL MICROVASCULAR ENDOTHELIAL-CELLS SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Article ID TUMOR NECROSIS FACTOR; RECOMBINANT GAMMA-INTERFERON; CYTOADHESIN INTEGRINS; IMMUNE INTERFERON; HUMAN FORESKIN; LYMPHOCYTES-T; HLA-DR; INTERLEUKIN-1; CULTURE; ACTIVATION AB Interactions between leukocytes and endothelial cells, particularly in the microvasculature, are important for the initiation and regulation of tissue inflammation. These interactions are regulated by the recognition of specific cell adhesion molecules (CAM) on both leukocytes and endothelial cells. In this study, we examined the modulation of cell surface expression of MHC antigens and the CAM intercellular adhesion molecule 1 (ICAM-1), lymphocyte function antigen 3 (LFA-3), and CD44 on human dermal microvascular endothelial cells (HDMEC) both grown in monolayers and differentiated into capillary-like structures on the basement membrane-like substrate matrigel. HDMEC grown in monolayers or differentiated on matrigel express comparable cell surface MHC class I, LFA-3, CD44, and ICAM-1. ICAM-1, but not LFA-3 or CD44, was increased in expression in a dose- and time-dependent manner by interleukin 1 (IL-1) alpha, tumor necrosis factor (TNF) alpha, lipopolysaccharide (LPS), or interferon (IFN) gamma. Comparable upregulation was observed both in cells grown in monolayers and cells differentiated on matrigel. IL-1 alpha, TNF alpha, and LPS increased ICAM-1 expression on average 100-200% whereas IFN gamma was somewhat less potent. Comparative studies with human umbilical vein endothelial cells (HUVEC) demonstrated consistently lower levels of ICAM-1 expression on HUVEC, but greater increases after cytokine stimulation. Pretreatment with dexamethasone or transforming growth factor (TGF) beta did not affect baseline expression of ICAM-1 or inhibit upregulation of ICAM-1 on HDMEC by IL-1 alpha, TNF alpha, LPS, or IFN gamma. Both IFN gamma and TNF alpha, but not IL-1 alpha increased MHC class I expression, whereas only IFN gamma induced the expression of HLA-DR on HDMEC. The effect of IL-1 alpha, TNF alpha, or IFN gamma was inhibited by antibody to the specific cytokine, but was unaffected by antibody to other cytokines. Additionally, IFN alpha or beta inhibited upregulation of HLA-DR by IFN gamma, but had no effect on the increased MHC class I or ICAM-1 expression mediated by this cytokine. These data demonstrate that the expression of CAM and MHC antigens on small vessel-derived endothelial cells is different from that observed on large-vessel HUVEC, is regulated by the presence of multiple cytokines operating via distinct pathways, and the expression and regulation of these proteins appear to be similar on cells that have been grown in monolayers to those morphologically differentiated into blood vessel-like structures. C1 NCI,DERMATOL BRANCH,BETHESDA,MD 20892. YAMANASHI UNIV,SCH MED,KOFU,YAMANASHI 400,JAPAN. RP SWERLICK, RA (reprint author), EMORY UNIV,SCH MED,DEPT DERMATOL,DRAWER 55,WMB RM 201,ATLANTA,GA 30322, USA. FU NIAMS NIH HHS [R01 AR 36632] NR 29 TC 120 Z9 120 U1 0 U2 1 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD AUG PY 1991 VL 97 IS 2 BP 190 EP 196 DI 10.1111/1523-1747.ep12479643 PG 7 WC Dermatology SC Dermatology GA FY296 UT WOS:A1991FY29600005 PM 1906507 ER PT J AU AMAGAI, M ELGART, GW KLAUSKOVTUN, V STANLEY, JR AF AMAGAI, M ELGART, GW KLAUSKOVTUN, V STANLEY, JR TI SOUTHERN ANALYSIS OF THE 230-KD BULLOUS PEMPHIGOID ANTIGEN GENE IN NORMAL HUMANS, ANIMALS, AND PATIENTS WITH JUNCTIONAL EPIDERMOLYSIS-BULLOSA SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Article ID HEMIDESMOSOME; LOCALIZATION; AUTOANTIBODIES; HETEROGENEITY; ANTIBODIES; CDNA AB To begin to characterize the 230-kD bullous pemphigoid antigen (BPA) gene, we performed Southern analysis on genomic DNA with probes derived from 7 kb of cDNA that spans most of the coding region of this hemidesmosomal plaque protein. When hybridized to a 1-kb fragment of this BPA cDNA, normal human genomic DNA digested with EcoRI, BamHI, PstI, HindIII, or EcoRV showed only a single band, which was unique for each enzyme, indicating a single human gene for BPA. To determine if a related gene exists in animals, we used probes covering the full 7 kb of cDNA for Southern analysis of genomic DNA from various vertebrates. A related gene was detected in other mammals (monkey, cow, dog, rabbit, mouse, and rat) but not in chicken, frog, or fish. Under these same hybridization conditions a probe for human beta-actin could detect an actin gene in all these species. Furthermore, immunofluorescence showed that an antibody raised against portions of the 230-kD BPA bound to the epidermal basement membrane of mammals but not that of a bird or amphibian. Finally, because most patients with junctional epidermolysis bullosa (JEB) have defective hemidesmosomes in ultrastructure, and probably function, we analyzed genomic DNA from these patients. No restriction fragment length polymorphisms (RFLP) were detected when the DNA from 11 normals and 8 JEB patients (representing 16 possible defective genes) was digested with BamHI, EcoRI, or PstI and hybridized to any part of the cDNA. These findings indicate that 1) there is a single BPA gene in humans; 2) a closely related gene exists in other mammals but not birds, amphibia, or fish; and 3) gross abnormalities of the BPA gene are not characteristic of JEB patients. RP AMAGAI, M (reprint author), NCI,DERMATOL BRANCH,BLDG 10,ROOM 12N238,BETHESDA,MD 20892, USA. RI Amagai, Masayuki/K-5325-2013 OI Amagai, Masayuki/0000-0003-3314-7052 NR 16 TC 16 Z9 16 U1 0 U2 1 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD AUG PY 1991 VL 97 IS 2 BP 249 EP 253 DI 10.1111/1523-1747.ep12480358 PG 5 WC Dermatology SC Dermatology GA FY296 UT WOS:A1991FY29600013 PM 1906508 ER PT J AU GRAYSON, NA DECOSTA, BR LINDERS, JTM RICE, KC AF GRAYSON, NA DECOSTA, BR LINDERS, JTM RICE, KC TI AN EXPEDIENT SYNTHESIS OF HIGH SPECIFIC ACTIVITY TRITIUM LABELED 4-FLUORO-1-[1-(2-THIENYL)CYCLOHEXYLPIPERIDINE ([H-3]FTCP), A LIGAND FOR FURTHER CHARACTERIZATION OF THE PHENCYCLIDINE/NMDA RECEPTOR COMPLEX SO JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS LA English DT Article DE PCP; TCP; TRITIATION; FLUOROTCP; NMDA RECEPTOR ID BINDING; FTCP AB [F-18]4-Fluoro-1-[1-(2-thienyl)cyclohexyl)]piperidine is a ligand potentially useful for positron emission tomography of the PCP/NMDA receptor complex in the mammalian brain. The tritium-labelled title compound was synthesized in 5 steps starting with cyclohexanone. Catalytic tritiolysis in the final step afforded the title compound ill high radiochemical yield and specific activity. This method represents a more efficient approach than previously published syntheses. RP GRAYSON, NA (reprint author), NIDDKD,MED CHEM LAB,DRUG DESIGN & SYNT SECT,BETHESDA,MD 20892, USA. NR 12 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0362-4803 J9 J LABELLED COMPD RAD JI J. Label. Compd. Radiopharm. PD AUG PY 1991 VL 29 IS 8 BP 919 EP 926 DI 10.1002/jlcr.2580290808 PG 8 WC Biochemical Research Methods; Chemistry, Medicinal; Chemistry, Analytical SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Chemistry GA GA359 UT WOS:A1991GA35900007 ER PT J AU POWERS, R GRONENBORN, AM CLORE, GM BAX, A AF POWERS, R GRONENBORN, AM CLORE, GM BAX, A TI 3-DIMENSIONAL TRIPLE-RESONANCE NMR OF C-13/N-15-ENRICHED PROTEINS USING CONSTANT-TIME EVOLUTION SO JOURNAL OF MAGNETIC RESONANCE LA English DT Note ID SPECTRA; PHASE; SPECTROSCOPY RP POWERS, R (reprint author), NATL INST DIABET & DIGEST & KIDNEY DIS,CHEM PHYS LAB,BETHESDA,MD 20892, USA. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 10 TC 168 Z9 169 U1 0 U2 6 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0022-2364 J9 J MAGN RESON PD AUG PY 1991 VL 94 IS 1 BP 209 EP 213 DI 10.1016/0022-2364(91)90312-H PG 5 WC Biochemical Research Methods; Physics, Atomic, Molecular & Chemical; Spectroscopy SC Biochemistry & Molecular Biology; Physics; Spectroscopy GA FZ542 UT WOS:A1991FZ54200024 ER PT J AU DAWSON, DA AF DAWSON, DA TI FAMILY-STRUCTURE AND CHILDRENS HEALTH AND WELL-BEING - DATA FROM THE 1988 NATIONAL-HEALTH INTERVIEW SURVEY ON CHILD HEALTH SO JOURNAL OF MARRIAGE AND THE FAMILY LA English DT Article ID MARITAL DISRUPTION; BEHAVIOR PROBLEMS; LIFE COURSE; DIVORCE; DISCORD; CONFLICT; MOTHERS C1 NATL CTR HLTH STAT,DIV HLTH INTERVIEW STAT,HYATTSVILLE,MD 20782. RP DAWSON, DA (reprint author), NIAAA,DIV BIOMETRY & EPIDEMIOL,5600 FISHERS LANE,RM 14C-26,ROCKVILLE,MD 20857, USA. NR 42 TC 162 Z9 162 U1 0 U2 10 PU NATL COUNC FAMILY RELATIONS PI MINNEAPOLIS PA 3989 CENTRAL AVE NE #550, MINNEAPOLIS, MN 55421 SN 0022-2445 J9 J MARRIAGE FAM JI J. Marriage Fam. PD AUG PY 1991 VL 53 IS 3 BP 573 EP 584 DI 10.2307/352734 PG 12 WC Family Studies; Sociology SC Family Studies; Sociology GA GD356 UT WOS:A1991GD35600005 ER PT J AU BACHRACH, CA LONDON, KA MAZA, PL AF BACHRACH, CA LONDON, KA MAZA, PL TI ON THE PATH TO ADOPTION - ADOPTION SEEKING IN THE UNITED-STATES, 1988 SO JOURNAL OF MARRIAGE AND THE FAMILY LA English DT Article ID FAMILY FORMATION; WHITE WOMEN; CHILDREN C1 NICHHD,BETHESDA,MD 20892. NATL CTR HLTH STAT,FAMILY GROWTH SURVEY BRANCH,HYATTSVILLE,MD 20782. ADM CHILDREN YOUTH & FAMILIES,BUR CHILDRENS,WASHINGTON,DC 20013. NR 22 TC 29 Z9 29 U1 0 U2 0 PU NATL COUNC FAMILY RELATIONS PI MINNEAPOLIS PA 3989 CENTRAL AVE NE #550, MINNEAPOLIS, MN 55421 SN 0022-2445 J9 J MARRIAGE FAM JI J. Marriage Fam. PD AUG PY 1991 VL 53 IS 3 BP 705 EP 718 DI 10.2307/352745 PG 14 WC Family Studies; Sociology SC Family Studies; Sociology GA GD356 UT WOS:A1991GD35600016 ER PT J AU THEOBALD, P PORTER, J RIVIER, C CORRIGAN, A HOOK, W SIRAGANIAN, R PERRIN, M VALE, W RIVIER, J AF THEOBALD, P PORTER, J RIVIER, C CORRIGAN, A HOOK, W SIRAGANIAN, R PERRIN, M VALE, W RIVIER, J TI NOVEL GONADOTROPIN-RELEASING-HORMONE ANTAGONISTS - PEPTIDES INCORPORATING MODIFIED N-OMEGA-CYANOGUANIDINO MOIETIES SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID HISTAMINE-RELEASE; LHRH; OVULATION; SEQUENCE; AGONISTS; POTENCY; ANALOGS; DESIGN; FSH; RAT AB In order to minimize the deleterious effects of histamine release resulting from the administration to rats and humans of some potent gonadotropin-releasing hormone (GnRH) antagonists, various arginine residues were replaced with the less basic N-omega-cyano-N-omega'-alkyl- or -arylhomoarginine, -arginine, or -p-aminophenylalanine and N-omega-triazolyllysine, -ornithine or -p-aminophenylalanine residues in active analogues. These novel analogues were synthesized on a solid-phase support via a two-step modification of the N-omega-NH2 of lysine, ornithine, or p-aminophenylalanine residues in otherwise protected resin bound peptides. Most analogues were tested in the rat antiovulatory assay (AOA) and three in vitro assays: a pituitary cell culture assay, a binding assay to pituitary cell membranes, and a histamine release assay. Introduction of the cyanoguanidino and N-omega-triazolyl moieties into GnRH analogues yielded several water-soluble antagonists which showed a desirable therapeutic ratio (low histamine release activity to high in vivo potency). Among them, ''Azaline'' (10, [Ac-DNal1,DCpa2,DPal3,Lys5(atz),DLys6(atz),ILys8,DAla10]GnRH), inhibited ovulation in the rat by 90% at 2-mu-g/rat with an ED50 in the in vitro histamine release assay comparable to that of GnRH itself. C1 SALK INST BIOL STUDIES,10010 N TORREY PINES RD,LA JOLLA,CA 92186. NIDR,IMMUNOL LAB,CLIN IMMUNOL SECT,BETHESDA,MD 20892. FU NICHD NIH HHS [N01-HD-9-2903] NR 29 TC 30 Z9 30 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD AUG PY 1991 VL 34 IS 8 BP 2395 EP 2402 DI 10.1021/jm00112a013 PG 8 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA GB158 UT WOS:A1991GB15800013 PM 1714956 ER PT J AU CUSHMAN, M NAGARATHNAM, D GOPAL, D CHAKRABORTI, AK LIN, CM HAMEL, E AF CUSHMAN, M NAGARATHNAM, D GOPAL, D CHAKRABORTI, AK LIN, CM HAMEL, E TI SYNTHESIS AND EVALUATION OF STILBENE AND DIHYDROSTILBENE DERIVATIVES AS POTENTIAL ANTICANCER AGENTS THAT INHIBIT TUBULIN POLYMERIZATION SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID ANTINEOPLASTIC AGENTS; COMBRETUM-CAFFRUM; ANTIMITOTIC AGENTS; BINDING-SITE; CELL-LINES; COLCHICINE; COMBRETASTATIN; PODOPHYLLOTOXIN; PICEATANNOL; ANALOGS AB An array of cis-, trans-, and dihydrostilbenes and some N-arylbenzylamines were synthesized and evaluated for their cytotoxicity in the five cancer cell cultures A-549 lung carcinoma, MCF-7 breast carcinoma, HT-29 colon adenocarcinoma, SKMEL-5 melanoma, and MLM melanoma. Several cis-stilbenes, structurally similar to combretastatins, were highly cytotoxic in all five cell lines and these were also found to be active as inhibitors of tubulin polymerization. The most active compounds also inhibited the binding of colchicine to tubulin. The most potent of the new compounds, both as a tubulin polymerization inhibitor and as a cytotoxic agent, was (Z)-1-(4-methoxyphenyl)-2-(3,4,5-trimethoxyphenyl)ethene (5a). This substance was almost as potent as combretastatin A-4 (1a), the most active of the combretastatins, as a tubulin polymerization inhibitor. Compound 5a was found to be approximately 140 times more cytotoxic against HT-29 colon adenocarcinoma cells and about 10 times more cytotoxic against MCF-7 breast carcinoma cells than combretastatin A-4. However, 5a was found to be about 20 times less cytotoxic against A-549 lung carcinoma cells, 30 times less cytotoxic against SKMEL-5 melanoma cells, and 7 times less cytotoxic against MLM melanoma cells than combretastatin A-4. The relative potencies 5a > 8a > 6a for the cis, dihydro, and trans compounds, respectively, as inhibitors of tubulin polymerization are in agreement with the relative potencies previously observed for combretastatin A-4 (1a), dihydrocombretastatin A-4 (1c), and trans-combretastatin A-4 (1b). The relative potencies 5a > 8a > 6a were also reflected in the results of the cytotoxicity assays. Structure-activity relationships of this group of compounds are also discussed. C1 NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. RP CUSHMAN, M (reprint author), PURDUE UNIV,DEPT MED CHEM & PHARMACOGNOSY,W LAFAYETTE,IN 47907, USA. FU NCI NIH HHS [CA47476, N01-CM-67699] NR 53 TC 262 Z9 270 U1 3 U2 34 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD AUG PY 1991 VL 34 IS 8 BP 2579 EP 2588 DI 10.1021/jm00112a036 PG 10 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA GB158 UT WOS:A1991GB15800036 PM 1875350 ER PT J AU KOVACS, T PARKANYI, L PELCZER, I CERVANTESLEE, F PANNELL, KH TORRENCE, PF AF KOVACS, T PARKANYI, L PELCZER, I CERVANTESLEE, F PANNELL, KH TORRENCE, PF TI SOLID-STATE AND SOLUTION CONFORMATION OF 3'-AMINO-3'-DEOXYTHYMIDINE, PRECURSOR TO A NONCOMPETITIVE INHIBITOR OF HIV-1 REVERSE-TRANSCRIPTASE SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; SUGAR RING CONFORMATION; X-RAY-ANALYSIS; CRYSTAL-STRUCTURES; AIDS VIRUS; 3'-AZIDO-3'-DEOXYTHYMIDINE TRIPHOSPHATE; AMINO ANALOGS; NUCLEOSIDE; AZT; 5'-TRIPHOSPHATE AB The recent finding that 3'-amino-3'-deoxythymidine 5'-triphosphate is a noncompetitive inhibitor of the HIV-1 reverse transcriptase (Kedar, P.S.; et al. Biochemistry 1990, 29, 3603-3611), prompted an investigation of the conformation of 3'-amino-3'-deoxythymidine. An X-ray diffraction study has revealed that the glycosidic torsion angle of the nucleoside is in the less common syn region and this solid-state geometry is stabilized by a three-dimensional network of self-associated hydrogen-bonded molecules. On the other hand, the aqueous solution conformation, as determined by H-1 NMR, places the glycosidic torsion angle in the more usual anti region with the sugar in an equilibrium between C3'-endo and C2'-endo puckering. The energy barrier between the solid-state and solution conformation is relatively low as was demonstrated by the MM2 calculations. C1 HUNGARIAN ACAD SCI,CENT RES INST CHEM,H-1025 BUDAPEST,HUNGARY. SYRACUSE UNIV,NUCL MAGNET RESONANCE & DATA PROC LAB,SYRACUSE,NY 13244. UNIV TEXAS,DEPT CHEM,EL PASO,TX 79968. RP KOVACS, T (reprint author), NIDDKD,MED CHEM LAB,BIOMED CHEM SECT,BETHESDA,MD 20892, USA. FU NCRR NIH HHS [RR-1317] NR 52 TC 24 Z9 24 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD AUG PY 1991 VL 34 IS 8 BP 2595 EP 2600 DI 10.1021/jm00112a038 PG 6 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA GB158 UT WOS:A1991GB15800038 PM 1714958 ER PT J AU IORIO, MA TOMASSINI, L MATTSON, MV GEORGE, C JACOBSON, AE AF IORIO, MA TOMASSINI, L MATTSON, MV GEORGE, C JACOBSON, AE TI SYNTHESIS, STEREOCHEMISTRY, AND BIOLOGICAL-ACTIVITY OF THE 1-(1-PHENYL-2-METHYLCYCLOHEXYL)PIPERIDINES AND THE 1-(1-PHENYL-4-METHYLCYCLOHEXYL)PIPERIDINES - ABSOLUTE-CONFIGURATION OF THE POTENT TRANS-(-)-1-(1-PHENYL-2-METHYLCYCLOHEXYL)PIPERIDINE SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID D-ASPARTATE RECEPTOR; PHENCYCLIDINE DERIVATIVES; ANTICONVULSANT MK-801; RAT-BRAIN; BINDING; CHANNEL; PCP; ANALOGS; INVIVO AB The (-)- and (+)-isomers of the cis- and trans-Ph/Me 1-(1-phenyl-2-methylcyclohexyl)piperidines have been synthesized and the achiral cis- and trans-Ph/Me 1-(1-phenyl-4-methylcyclohexyl)piperidines were prepared, and their in vitro [displacement of [H-3]TCP (1-(2-thienylcyclohexyl)]piperidine) from the PCP (1-(1-phenylcyclohexyl)piperidine) binding site] and in vivo (rotarod assay) activities determined. The 1-(1-phenyl-2-methylcyclohexyl)piperidine isomers were resolved by classical crystallization procedures, through the diastereomeric salts obtained with d- and l-10-camphorsulfonic acid. The relative stereochemistry of the cis- and trans-Ph/Me 1-(1-phenyl-2-methylcyclohexyl)piperidines and the achiral cis- and trans-Ph/Me 1-(1-phenyl-4-methylcyclohexyl)piperidines was established by using C-13 and H-1 NMR. Both (-)-trans-1-(1-phenyl-2-methylcyclohexyl)piperidine ((-)-2) and (+)-trans-1-(1-phenyl-2-methylcyclohexyl)piperidine ((+)-2) were examined by single-crystal X-ray analysis, and the absolute configuration of (-)-2 was determined to be 1S,2R. The (-)-2 was found to be about five times more potent than PCP in vitro and twice as potent in vivo. It is the most potent of all of the simple methyl-substituted cyclohexyl PCP isomers and is among the most potent PCP-like compounds which have been synthesized. It was nine times more potent in vitro and four times more potent in vivo than (+)-2. The racemic cis-1-(1-phenyl-2-methylcyclohexyl)piperidine (3), and its enantiomers ((+)-3 and (-)-3), were essentially inactive in vitro and in vivo. The cis-Ph/Me 1-(1-phenyl-4-methylcyclohexyl)piperidine (18) was more potent than trans-Ph/Me 1-(1-phenyl-4-methylcyclohexyl)piperidine (17), but considerably less potent than (-)-2. The enantioselectivity observed at the PCP binding site for (-)-2 could indicate that this site can discriminate between enantiotopic edges of the achiral PCP (choosing the pro-1-S edge), as does the mu-opioid receptor in the prodine series of opioids. Benzimidoyl or benzoyl group replacement of the phenyl ring in the 1-(1-phenyl-2-methylcyclohexyl)piperidine series gave compounds which showed little in vitro and in vivo activity. C1 NIDDKD,MED CHEM LAB,BETHESDA,MD 20892. IST SUPER SANITA,PHARMACEUT CHEM LAB,I-00161 ROME,ITALY. USN,RES LAB,WASHINGTON,DC 20375. NR 33 TC 13 Z9 13 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD AUG PY 1991 VL 34 IS 8 BP 2615 EP 2623 DI 10.1021/jm00112a041 PG 9 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA GB158 UT WOS:A1991GB15800041 PM 1875352 ER PT J AU PAYZA, K RUSSELL, JT AF PAYZA, K RUSSELL, JT TI ACTIVATION AND INACTIVATION OF OXYTOCIN AND VASOPRESSIN RELEASE FROM ISOLATED NERVE-ENDINGS (NEUROSECRETOSOMES) OF THE RAT NEUROHYPOPHYSIS SO JOURNAL OF NEUROCHEMISTRY LA English DT Article DE SECRETION; NEUROSECRETOSOMES; IONOMYCIN ID HORMONE-RELEASE; NEUROTRANSMITTER RELEASE; CALCIUM; TERMINALS; SECRETION; DEPOLARIZATION; NEURONS; NEUROPHYSIN; INCREASE; ABSENCE AB Neurosecretory terminals (neurosecretosomes, NSS) were isolated from rat neurohypophyses. High [K+]o or veratridine stimulated secretion of vasopressin and oxytocin by up to approximately 100-fold. Stimulated secretion was dependent on calcium and temperature, and could be elicited from NSS maintained in culture for 4 days. After overnight culture of the NSS, secretion was still inhibited by calcium channel blockers (cobalt, dihydropyridines, omega-conotoxin, D 600) and kappa-opiates (dynorphin and U50488). Ionomycin evoked dose- and calcium-dependent hormone release, with a Hill coefficient for calcium of 1.74. High [K+]o enhanced the 5-mu-M ionomycin-induced secretion, apparently through calcium entry rather than depolarization, as the increase in secretion was abolished by 100-mu-M D 600. During prolonged depolarization the hormone secretion peaked within 2 min, then declined to near basal levels. Depolarization for 25 min without calcium neither activated secretion nor prevented subsequent secretion on readdition of calcium, suggesting that the decline in secretion was not due to membrane depolarization. Indeed, the rates of decline in secretion were similar for different levels of depolarization (0.070 +/- 0.003 and 0.081 +/- 0.003 min-1 for 25 and 45 mM [K+]o, respectively). Four minutes after the onset of continuous depolarization (45 mM [K+]o) in the presence of calcium, the declining secretion was still dependent on voltage-activated calcium influx through channels sensitive to D 600 and nitrendipine. The results presented here suggest that the decline in secretion during prolonged deplarizing stimuli may be due to exhaustion, inactivation, or desensitization of a calcium-triggered event. C1 NICHHD,DEV NEUROBIOL LAB,NEURONAL SECRETORY SYST UNIT,BLDG 36,RM B-316,BETHESDA,MD 20892. NR 47 TC 2 Z9 2 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PD AUG PY 1991 VL 57 IS 2 BP 499 EP 508 DI 10.1111/j.1471-4159.1991.tb03779.x PG 10 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA FX907 UT WOS:A1991FX90700019 PM 2072100 ER PT J AU GAWNE, TJ RICHMOND, BJ OPTICAN, LM AF GAWNE, TJ RICHMOND, BJ OPTICAN, LM TI INTERACTIVE EFFECTS AMONG SEVERAL STIMULUS PARAMETERS ON THE RESPONSES OF STRIATE CORTICAL COMPLEX CELLS SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID TWO-DIMENSIONAL PATTERNS; INFERIOR TEMPORAL CORTEX; PRIMARY VISUAL-CORTEX; SINGLE UNITS; 2-DIMENSIONAL PATTERNS; CONTRAST; NEURONS; MONKEY AB 1. Although neurons within the visual system are often described in terms of their responses to particular patterns such as bars and edges, they are actually sensitive to many different stimulus features, such as the luminances making up the patterns and the duration of presentation. Many different combinations of stimulus parameters can result in the same neuronal response, raising the problem of how the nervous system can extract information about visual stimuli from such inherently ambiguous responses. It has been shown that complex cells transmit significant amounts of information in the temporal modulation of their responses, raising the possibility that different stimulus parameters are encoded in different aspects of the response. To find out how much information is actually available about individual stimulus parameters, we examined the interactions among three stimulus parameters in the temporally modulated responses of striate cortical complex cells. 2. Sixteen black and white patterns were presented to two awake monkeys at each of four luminance-combinations and five durations, giving a total of 320 unique stimuli. Complex cells were recorded in layers 2 and 3 of striate cortex, with the stimuli centered on the receptive fields as determined by mapping with black and white bars. 3. An analysis of variance (ANOVA) was applied to these data with the three stimulus parameters of pattern, the luminance-combinations, and duration as the independent variables. The ANOVA was repeated with the magnitude and three different aspects of the temporal modulation of the response as the dependent variables. For the 19 neurons studied, many of the interactions between the different stimulus parameters were statistically significant. For some response measures the interactions accounted for more than one-half of the total response variance. 4. We also analyzed the stimulus-response relationships with the use of information theoretical techniques. We defined input codes on the basis of each stimulus parameter alone, as well as their combinations, and output codes on the basis of response strength, and on three measures of temporal modulation, also taken individually and together. Transmitted information was greatest when the response of a neuron was interpreted as a temporally modulated message about combinations of all three stimulus parameters. The interaction terms of the ANOVA suggest that the response of a complex cell can only be interpreted as a message about combinations of all three stimulus parameters. However, the information about a single parameter was not completely obscured even when the other parameters were varied, i.e., the response of a complex cell could be interpreted as a message about any single stimulus parameter. 5. The three-part temporal code carried at least twice as much stimulus-dependent information as the response strength alone. Every aspect of the response carried more information about the Stimulus pattern than about any other stimulus parameter. No single aspect of the response was dedicated to any particular stimulus parameter. However, the proportion of the information about the luminance-combinations and duration was greater in the temporal modulation of the response than in the response strength. 6. The significant interactions among stimulus parameters shown by the ANOVA means that the effect on a neuronal response of changing one stimulus parameter is a function of the values of the other parameters. Thus, the value of any single stimulus parameter cannot be determined from a neuronal response by simply subtracting the effects of the other stimulus parameters, as a linear model would suggest. However, information theory does not assume a linear model, and it showed that with a nonlinear system the response of a neuron can be interpreted as a message about single stimulus parameters and not just about combinations of parameters. C1 NEI,SENSORIMOTOR RES,BETHESDA,MD 20892. RP GAWNE, TJ (reprint author), NIMH,NEUROPSYCHOL LAB,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 31 TC 20 Z9 20 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD AUG PY 1991 VL 66 IS 2 BP 379 EP 389 PG 11 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA GA524 UT WOS:A1991GA52400003 PM 1774579 ER PT J AU ROBINSON, DL MCCLURKIN, JW KERTZMAN, C PETERSEN, SE AF ROBINSON, DL MCCLURKIN, JW KERTZMAN, C PETERSEN, SE TI VISUAL RESPONSES OF PULVINAR AND COLLICULAR NEURONS DURING EYE-MOVEMENTS OF AWAKE, TRAINED MACAQUES SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID MONKEY SUPERIOR COLLICULUS; REAL-MOTION CELLS; INDUCED STIMULUS MOVEMENT; RHESUS-MONKEY; INFERIOR PULVINAR; MACACA-MULATTA; BEHAVING MONKEYS; PRIMATE PULVINAR; RECEPTIVE-FIELDS; UNIT-ACTIVITY AB 1. We recorded from single neurons in awake, trained rhesus monkeys in a lighted environment and compared responses to stimulus movement during periods of fixation with those to motion caused by saccadic or pursuit eye movements. Neurons in the inferior pulvinar (PI), lateral pulvinar (PL), and superior colliculus were tested. 2. Cells in PI and PL respond to stimulus movement over a wide range of speeds. Some of these cells do not respond to comparable stimulus motion, or discharge only weakly, when it is generated by saccadic or pursuit eye movements. Other neurons respond equivalently to both types of motion. Cells in the superficial layers of the superior colliculus have similar properties to those in PI and PL. 3. When tested in the dark to reduce visual stimulation from the background, cells in PI and PL still do not respond to motion generated by eye movements. Some of these cells have a suppression of activity after saccadic eye movements made in total darkness. These data suggest that an extraretinal signal suppresses responses to visual stimuli during eye movements. 4. The suppression of responses to stimuli during eye movements is not an absolute effect. Images brighter than 2.0 log units above background illumination evoke responses from cells in PI and PL. The suppression appears stronger in the superior colliculus than in PI and PL. 5. These experiments demonstrate that many cells in PI and PL have a suppression of their responses to stimuli that cross their receptive fields during eye movements. These cells are probably suppressed by an extraretinal signal. Comparable effects are present in the superficial layers of the superior colliculus. These properties in PI and PL may reflect the function of the ascending tectopulvinar system. RP ROBINSON, DL (reprint author), NEI,SENSORIMOTOR RES LAB,VISUAL BEHAV SECT,BLDG 10,ROOM 10C101,BETHESDA,MD 20892, USA. NR 53 TC 36 Z9 36 U1 1 U2 2 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD AUG PY 1991 VL 66 IS 2 BP 485 EP 496 PG 12 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA GA524 UT WOS:A1991GA52400010 PM 1774583 ER PT J AU MASCO, D VANDEWALLE, M SPIEGEL, S AF MASCO, D VANDEWALLE, M SPIEGEL, S TI INTERACTION OF GANGLIOSIDE GM1 WITH THE B-SUBUNIT OF CHOLERA-TOXIN MODULATES GROWTH AND DIFFERENTIATION OF NEUROBLASTOMA N18 CELLS SO JOURNAL OF NEUROSCIENCE LA English DT Article ID NEURO-BLASTOMA CELLS; PROTEIN-KINASE-C; ANTIGANGLIOSIDE ANTIBODIES; ENDOGENOUS GANGLIOSIDE; CULTURED-CELLS; RAT-BRAIN; PROLIFERATION; OUTGROWTH; CALCIUM; NEURITOGENESIS AB The present study uses the B subunit of cholera toxin, a protein that binds specifically to ganglioside GM1, to examine the role of endogenous GM1 in the process of growth and differentiation of mouse neuroblastoma N18 cells. Binding of the B subunit to neuroblastoma N18 cells inhibited DNA synthesis with concomitant induction of differentiation. The B subunit induced pronounced morphological changes: an increase in neurite outgrowth with branched neurites and spinelike processes. The distinct morphological alterations and neuritogenesis in response to the B subunit were also revealed by immunofluorescence with fluorescein-labeled B subunit. The mechanism of the B subunit-induced differentiation is different than that of spontaneous differentiation. Thrombin, a serine protease present in normal serum, inhibits neurite outgrowth induced by the removal of serum from the medium. In contrast, thrombin did not cause retraction of the neurites induced by the B subunit. Thus, thrombin or a thrombin-like protease is not involved in the process of neurite outgrowth mediated through endogenous GM1. The biological effects of the B subunit are due to the binding of the B subunit to ganglioside GM1 and not due to changes in cAMP levels resulting from contaminating A subunit. We used highly purified cloned B subunit that cannot contain any A subunit because it was isolated from a Vibrio cholerae mutant that only expresses the B subunit. Neither the cloned nor commercial preparations of the B subunit induced increases of cAMP in these cells. There was a good correlation between the amount of B subunit bound to the cells and the biological effect. Finally, treatment with neuraminidase, which caused a fourfold increase in the level of membrane GM1 as determined by iodinated cholera toxin binding, enhanced the biological effect of the B subunit. However, neuraminidase treatment alone did not have significant effects, either on DNA synthesis or on morphology of the cells, indicating that elevations in the level of GM1 per se are not sufficient by themselves to cause significant changes in cell growth or differentiation. It seems most likely that the aggregation of endogenous GM1 on the cell surface by the B subunit is responsible for these effects on mouse neuroblastoma N18 cells. C1 GEORGETOWN UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,357 BASIC SCI BLDG,3900 RESERVOIR RD NW,WASHINGTON,DC 20007. NIDDK,LCDB,BIOTECHNOL UNIT,BETHESDA,MD 20892. FU NIGMS NIH HHS [1 R 29 GM 39718, 1 R 01 GM 43880] NR 62 TC 36 Z9 36 U1 0 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD AUG PY 1991 VL 11 IS 8 BP 2443 EP 2452 PG 10 WC Neurosciences SC Neurosciences & Neurology GA GB020 UT WOS:A1991GB02000017 PM 1651376 ER PT J AU OPARA, EC HUBBARD, VS BURCH, WM AKWARI, OE AF OPARA, EC HUBBARD, VS BURCH, WM AKWARI, OE TI HOMOLOGOUS DESENSITIZATION OF PANCREATIC BETA-CELLS TO GLUCOSE RESPONSE BY POLYUNSATURATED FATTY-ACIDS SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Note DE DESENSITIZED ISLETS; FATTY ACIDS; INSULIN SECRETION ID DEPENDENT DIABETES-MELLITUS; INDUCED INSULIN-SECRETION; ARACHIDONIC-ACID; RAT ISLETS; B-CELL; CYSTIC-FIBROSIS; MOUSE ISLETS; RELEASE; GLUCAGON; HYPERGLYCEMIA AB We have shown recently that a mixture of 10 mM linoleic acid (18:2, omega-6) and 5 mM linolenic acid (18:3, omega-3) enhanced insulin secretion by microdissected islets. In the present study, our aim was to examine the effect of a prestimulus with this mixture of 10 mM linoleic acid and 5 mM linolenic acid on glucose and arginine-stimulated insulin response, by perifused isolated islets. Insulin secretion by untreated (control) islets estimated as integrated area under the curve (AUC/20 mins) above basal during three pulses of 27.7 mM glucose separated by 20 min periods of basal (washout) perifusions were 2950 +/- 585 pg, 5185 +/- 1258 pg, and 2410 +/- 921 pg, a greater response (P < 0.03) being obtained during the second glucose challenge. The insulin response of the islets to the glucose challenge was blunted profoundly if the islets were first exposed to a 20 min period of perifusion with the fatty acid mixture followed by a 20 min washout. Thus, the insulin AUC/20 min decreased from a control value of 2410 +/- 921 pg to 337 +/- 201 pg (P < 0.03) after prestimulus with the fatty acids. In contrast, fatty acid exposed islets were still responsive to 10 mM arginine. These data suggest that (1) a mixture of 10 mM linoleic acid and 5 mM linolenic acid can desensitize isolated perifused islets to glucose but not arginine; and (2) glucose intolerance associated with lipemia may result, at least in part, from a direct effect on the islets of Langerhans. C1 NIDDKD,BETHESDA,MD. RP OPARA, EC (reprint author), DUKE UNIV,MED CTR,DEPT SURG,POB 3076,DURHAM,NC 27710, USA. NR 47 TC 9 Z9 9 U1 3 U2 3 PU BUTTERWORTH-HEINEMANN PI WOBURN PA 225 WILDWOOD AVE #UNITB PO BOX 4500, WOBURN, MA 01801-2084 SN 0955-2863 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD AUG PY 1991 VL 2 IS 8 BP 424 EP 429 DI 10.1016/0955-2863(91)90111-H PG 6 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA GA084 UT WOS:A1991GA08400002 ER PT J AU TSUCHIMOCHI, M HIGASHINO, N OKANO, A KATO, J AF TSUCHIMOCHI, M HIGASHINO, N OKANO, A KATO, J TI STUDY OF COMBINED TECHNETIUM 99M METHYLENE DIPHOSPHONATE AND GALLIUM 67 CITRATE SCINTIGRAPHY IN DIFFUSE SCLEROSING OSTEOMYELITIS OF THE MANDIBLE - CASE-REPORTS SO JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY LA English DT Article ID CONCISE COMMUNICATION; INFLAMMATORY LESIONS; SEPTIC ARTHRITIS; TC-99M PHOSPHATE; DIAGNOSIS; GA-67; GA-67-CITRATE; MECHANISM; ABSCESSES; CHILDREN C1 NIDR,DIAGNOST SYST BRANCH,BETHESDA,MD 20892. RP TSUCHIMOCHI, M (reprint author), NIPPON DENT UNIV,SCH DENT NIIGATA,DEPT ORAL & MAXILLOFACIAL SURG 2,1-8 HAMAURA CHO,NIIGATA 951,JAPAN. NR 42 TC 16 Z9 16 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0278-2391 J9 J ORAL MAXIL SURG JI J. Oral Maxillofac. Surg. PD AUG PY 1991 VL 49 IS 8 BP 887 EP 897 DI 10.1016/0278-2391(91)90024-G PG 11 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA FZ160 UT WOS:A1991FZ16000024 PM 2072205 ER PT J AU WISWELL, TE CURTIS, J DOBEK, AS ZIERDT, CH AF WISWELL, TE CURTIS, J DOBEK, AS ZIERDT, CH TI STAPHYLOCOCCUS-AUREUS COLONIZATION AFTER NEONATAL CIRCUMCISION IN RELATION TO DEVICE USED SO JOURNAL OF PEDIATRICS LA English DT Article ID 1ST MONTH; CLAMP; LIFE C1 WALTER REED ARMY MED CTR, DEPT CLIN INVEST, WASHINGTON, DC 20307 USA. NIH, DEPT CLIN PATHOL, BETHESDA, MD 20892 USA. RP WISWELL, TE (reprint author), WALTER REED ARMY MED CTR, DEPT PEDIAT, NEONATOL SERV, WASHINGTON, DC 20307 USA. NR 15 TC 5 Z9 5 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-3476 EI 1097-6833 J9 J PEDIATR-US JI J. Pediatr. PD AUG PY 1991 VL 119 IS 2 BP 302 EP 304 DI 10.1016/S0022-3476(05)80749-8 PG 3 WC Pediatrics SC Pediatrics GA FZ692 UT WOS:A1991FZ69200025 PM 1861220 ER PT J AU FRANCE, CP WINGER, G MEDZIHRADSKY, F SEGGEL, MR RICE, KC WOODS, JH AF FRANCE, CP WINGER, G MEDZIHRADSKY, F SEGGEL, MR RICE, KC WOODS, JH TI MIRFENTANIL - PHARMACOLOGICAL PROFILE OF A NOVEL FENTANYL DERIVATIVE WITH OPIOID AND NONOPIOID EFFECTS SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID TREATED RHESUS-MONKEYS; MORPHINE; NALTREXONE; RECEPTORS; BINDING; PIGEONS; SALINE AB Mirfentanil [N-(2-pyrazinyl)-N-(1-phenethyl-4-piperidinyl)-2-furamide] was studied for its binding affinity in isolated neuronal membranes, and for its effects in vivo. In binding to opioid receptors in monkey brain membranes, mirfentanil was much more selective for mu sites (7.99 nM) than for either kappa (1428 nM) or delta (480 nM) sites as measured by displacement of [H-3]DAMGO, [H-3]U-69,593 or [H-3]DPDPE, respectively. In morphine-treated pigeons discriminating among naltrexone, saline and morphine, mirfentanil failed to substitute for either training drug; in morphine-abstinent pigeons, mirfentanil reversed responding on the naltrexone key (i.e., reversed withdrawal). In morphine-treated monkeys discriminating between saline and naltrexone, mirfentanil substituted completely for naltrexone, and this effect was attenuated by an acute injection of morphine; mirfentanil also attenuated the withdrawal-reversing effects of alfentanil in morphine-abstinent monkeys. Administered i.v., mirfentanil maintained rates of self-administration responding only slightly below rates maintained by alfentanil, and this effect of mirfentanil was antagonized by quadazocine. Small doses of mirfentanil (0.032-0.32 mg/kg) antagonized the analgesic effects of alfentanil; larger doses of mirfentanil both antagonized the analgesic effects of alfentanil and produced analgesic effects when administered alone. The analgesic effects of mirfentanil were not attenuated by large doses of opioid antagonists. Mirfentanil had modest respiratory depressant effects that were not altered by quadazocine; however, mirfentanil antagonized the respiratory depressant effects of large doses of alfentanil. Both in vivo and in vitro, mirfentanil appears to have selectivity for opioid mu receptors. Moreover, at doses larger than those which exert opioid effects, mirfentanil has nonopioid analgesic effects. Together these results demonstrate a novel pharmacological profile for mirfentanil: it is a selective, low-efficacy opioid mu agonist with nonopioid analgesic effects in monkeys. C1 UNIV MICHIGAN,DEPT PHARMACOL,ANN ARBOR,MI 48109. UNIV MICHIGAN,DEPT PSYCHOL,ANN ARBOR,MI 48109. UNIV MICHIGAN,DEPT BIOL CHEM,ANN ARBOR,MI 48109. NIDDKD,MED CHEM LAB,BETHESDA,MD. FU NIDA NIH HHS [DA 00254, DA 05018] NR 18 TC 30 Z9 31 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD AUG PY 1991 VL 258 IS 2 BP 502 EP 510 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GB325 UT WOS:A1991GB32500017 PM 1650830 ER PT J AU DURCAN, MJ LISTER, RG LINNOILA, M AF DURCAN, MJ LISTER, RG LINNOILA, M TI EVIDENCE FOR CENTRAL ALPHA-2 ADRENOCEPTORS, NOT IMIDAZOLINE BINDING-SITES, MEDIATING THE ETHANOL-ATTENUATING PROPERTIES OF ALPHA-2 ADRENOCEPTOR ANTAGONISTS SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID ALPHA-2-ADRENOCEPTOR ANTAGONIST; H-3 RAUWOLSCINE; DEPENDENT RATS; BRAIN; L-659,066; MICE; NOREPINEPHRINE; HETEROGENEITY; IDAZOXAN; RELEASE AB The role of central adrenoceptors in the ethanol-attenuating effects of alpha-2 adrenoceptor blockers was investigated in mice; the centrally active antagonist atipamezole was compared with L 659,066, which penetrates the brain poorly. L 659,066 (1-10 mg/kg) had no effect on the hypothermia induced by ethanol (2 g/kg) or ethanol ataxia (2.4 g/kg), whereas atipamezole (1 mg/kg) significantly attenuated both ethanol-induced hypothermia and ataxia. Atipamezole (1-3 mg/kg) significantly attenuated the ethanol-induced reduction in exploratory head-dipping in a holeboard test whereas L 659,066 was only effective at a dose of 1 mg/kg, higher doses (3 and 10 mg/kg) and a lower dose (0.3 mg/kg) were ineffective. Atipamezole was without effect on ethanol's locomotor stimulant effect in the holeboard but L 659,066 attenuated this effect at doses < 3 mg/kg. Many alpha-2 adrenoceptor ligands also have affinity for nonadrenergic imidazoline-binding sites. The role these sites may play in attenuating ethanol's effects was investigated by comparing RX 821002 (methoxy idazoxan), which has little or no affinity for imidazoline-binding sites with atipamezole. Both atipamezo1e (1 mg/kg) and RX 821002 (0.06-0.2 mg/kg) significantly attenuated ethanol-induced hypothermia, ataxia and reduction in head-dipping, but were without effect on ethanol-induced locomotor stimulation. These results suggest that nonadrenergic imidazoline-binding sites are not implicated in the ethanol-attenuating properties of alpha-2 adrenoceptor antagonists. RP DURCAN, MJ (reprint author), NIAAA,DIV INTRAMURAL CLIN & BIOL RES,CLIN STUDIES LAB,BLDG 10,ROOM 3C102,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 31 TC 11 Z9 11 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD AUG PY 1991 VL 258 IS 2 BP 576 EP 582 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GB325 UT WOS:A1991GB32500027 PM 1678013 ER PT J AU MANJULA, BN KHANDKE, KM FAIRWELL, T RELF, WA SRIPRAKASH, KS AF MANJULA, BN KHANDKE, KM FAIRWELL, T RELF, WA SRIPRAKASH, KS TI HEPTAD MOTIFS WITHIN THE DISTAL SUBDOMAIN OF THE COILED-COIL ROD REGION OF M-PROTEIN FROM RHEUMATIC-FEVER AND NEPHRITIS ASSOCIATED SEROTYPES OF GROUP-A STREPTOCOCCI ARE DISTINCT FROM EACH OTHER - NUCLEOTIDE-SEQUENCE OF THE M57 GENE AND RELATION OF THE DEDUCED AMINO-ACID-SEQUENCE TO OTHER M-PROTEINS SO JOURNAL OF PROTEIN CHEMISTRY LA English DT Article DE STREPTOCOCCAL M-PROTEIN; COILED-COIL; SUBDOMAINS; HEPTAD MOTIF; RHEUMATIC FEVER; NEPHRITIS ID TYPE-6 M PROTEIN; ACUTE GLOMERULONEPHRITIS; INTERMEDIATE FILAMENTS; PEP-M5 PROTEIN; PYOGENES; DESIGN; IDENTIFICATION; EPIDEMIOLOGY; INFECTIONS; MOLECULES AB Streptococcal M protein, a dimeric alpha helical coiled-coil molecule, is an antigenically variable virulence factor on the surface of the bacteria. Our recent conformational analysis of the complete sequence of the M6 protein led us to propose a basic model for the M protein consisting of an extended central coiled-coil rod domain flanked by a variable N-terminal and a conserved C-terminal end domains. The central coiled-coil rod domain of M protein, which constitutes the major part of the M molecule, is made up of repeating heptads of the generalized sequence a-b-c-d-e-f-g, wherein "a" and "d" are predominantly apolar residues. Based on the differences in the heptad pattern of apolar residues and internal sequence homology, the central coiled-coil rod domain of M protein could be further divided into three subdomains I, II, and III. The streptococcal sequelae rheumatic fever (RF) and acute glomerulonephritis (AGN) have been known to be associated with distinct serotypes. Consistent with this, we observed that the AGN associated M49 protein exhibits a heptad motif that is distinct from the RF associated M5 and M6 proteins. Asn and Leu predominated in the "a" and "d" positions, respectively, in subdomain I of the M5 and M6 proteins, whereas apolar residues predominated in both these positions in the M49 protein. To establish whether the heptad motif of M49 is unique to this protein, or is a general characteristic of nephritis-associated serotypes, the amino acid sequence of M57, another nephritis-associated serotype, has now been examined. The gene encoding M57 was amplified by PCR, cloned into pUC19 vector, and sequenced. The C-terminal half of M57 is highly homologous to other M proteins (conserved region). In contrast, its N-terminal half (variable region) revealed no significant homology with any of the M proteins. Heptad periodicity analysis of the M57 sequence revealed that the basic design principles, consisting of distinct domains observed in the M6 protein, are also conserved in the M57 molecule. However, the heptad motif within the coiled-coil subdomain I of M57 was distinct from M5 and M6 but similar to M49. Similar analyses of the heptad characteristics within the reported sequences of M1, M12, and M24 proteins further confirmed the conservation of the overall architectural design of sequentially distinct M proteins. Furthermore, the heptad motif within subdomain I of the AGN-associated serotypes M1 and M12 was similar to M49 and M57, whereas that of the RF associated M24 was similar to the M5 and M6 proteins. These results clearly demonstrate a correlation between the heptad motifs within the distal coiled-coil subdomain of the M proteins from different streptococcal serotypes and their epidemiological association with the sequelae AGN and RF. C1 NHLBI,BETHESDA,MD 20892. MENZIES SCH HLTH RES,CASUARINA,NT 0811,AUSTRALIA. RP MANJULA, BN (reprint author), ROCKEFELLER UNIV,NEW YORK,NY 10021, USA. FU NHLBI NIH HHS [HL36025] NR 50 TC 30 Z9 30 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0277-8033 J9 J PROTEIN CHEM JI J. Protein Chem. PD AUG PY 1991 VL 10 IS 4 BP 369 EP 384 DI 10.1007/BF01025251 PG 16 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GG782 UT WOS:A1991GG78200002 PM 1781883 ER PT J AU KOCSIS, E TRUS, BL STEER, CJ BISHER, ME STEVEN, AC AF KOCSIS, E TRUS, BL STEER, CJ BISHER, ME STEVEN, AC TI IMAGE AVERAGING OF FLEXIBLE FIBROUS MACROMOLECULES - THE CLATHRIN TRISKELION HAS AN ELASTIC PROXIMAL SEGMENT SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article ID TRANSMISSION ELECTRON-MICROSCOPY; COATED VESICLES; LIGHT-CHAIN; HEAVY-CHAIN; PROTEIN; MICROGRAPHS; FILAMENTS; PHOSPHORYLATION; TRIMERS; LIVER C1 NIAMSD,STRUCT BIOL LAB,BETHESDA,MD 20892. NIH,DIV COMP RES & TECHNOL,COMP SYST LAB,BETHESDA,MD 20892. UNIV MINNESOTA,SCH MED,DEPT MED,MINNEAPOLIS,MN 55455. NR 55 TC 108 Z9 110 U1 0 U2 4 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PD AUG PY 1991 VL 107 IS 1 BP 6 EP 14 DI 10.1016/1047-8477(91)90025-R PG 9 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA GC975 UT WOS:A1991GC97500002 PM 1817611 ER PT J AU LEWIS, JF MARON, BJ AF LEWIS, JF MARON, BJ TI HYPERTROPHIC CARDIOMYOPATHY CHARACTERIZED BY MARKED HYPERTROPHY OF THE POSTERIOR LEFT-VENTRICULAR FREE WALL - SIGNIFICANCE AND CLINICAL IMPLICATIONS SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID SYSTOLIC ANTERIOR MOTION; TWO-DIMENSIONAL ECHOCARDIOGRAPHY; MUSCULAR SUBAORTIC STENOSIS; SUB-AORTIC STENOSIS; OUTFLOW OBSTRUCTION; MITRAL-VALVE; PRESSURE-GRADIENT; PATHO-PHYSIOLOGY; WIDE-ANGLE; M-MODE AB This report describes a subgroup of 17 patients with hypertrophic cardiomyopathy and an unusual and distinctive pattern of left ventricular hypertrophy characterized on echocardiography by marked thickening of the posterior left ventricular free wall and virtually normal or only modestly increased ventricular septal thickness. This distribution of hypertrophy often created a distinctive pattern of "inverted" asymmetry of the posterior wall relative to the septum. The thickness of the posterior wall was 20 to 42 mm (mean 25), while that of the basal ventricular septum was only 12 to 24 mm (mean 17). The left ventricular outflow tract was narrowed because of anterior displacement of the mitral valve within the small left ventricular cavity. Systolic anterior motion of The mitral valve was present in 16 of the 17 patients. The patients ranged in age from 13 to 54 years (mean 31) at most recent evaluation; most (11 of 17, 65%) were severely symptomatic and had experienced important symptoms early in life (before age 40). The condition of only 4 of these 11 patients improved with medical therapy over an average follow-up period of 9 years; however, 6 of the 7 patients who had unsuccessful medical treatment and underwent operation with mitral valve replacement (5 patients) or ventricular septal myotomy-myectomy (1 patient) experienced symptomatic benefit from surgery. The subgroup of patients described in this report underscores the morphologic and clinical diversity that exists within the overall disease spectrum of hypertrophic cardiomyopathy. Characteristically, the patients were young, severely symptomatic and demonstrated evidence of outflow obstruction and an "inverted" asymmetric pattern or posterior free wall left ventricular hypertrophy. Because of their relatively modest ventricular septal hypertrophy, mitral valve replacement (rather than myotomy-myectomy) may be the operative procedure of choice in such patients with obstructive hypertrophic cardiomyopathy. C1 HOWARD UNIV,COLL MED,DEPT MED,DIV CARDIOVASC DIS,WASHINGTON,DC 20001. RP LEWIS, JF (reprint author), NHLBI,CARDIOL BRANCH,ECHOCARDIOG LAB,BLDG 10,ROOM 7B-15,BETHESDA,MD 20892, USA. FU NHLBI NIH HHS [HL01984] NR 39 TC 16 Z9 17 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD AUG PY 1991 VL 18 IS 2 BP 421 EP 428 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA FY965 UT WOS:A1991FY96500026 PM 1856409 ER PT J AU BROWN, LJ AF BROWN, LJ TI DENTAL AMALGAM - DESIGNS FOR RESEARCH IN HUMAN-POPULATIONS SO JOURNAL OF THE AMERICAN DENTAL ASSOCIATION LA English DT Article ID ORAL AIR MERCURY; VAPOR; EXPOSURE; DENTISTS; FILLINGS; BLOOD; RESTORATIONS; HEALTH; SYMPTOMS; RELEASE AB Questions have been raised about the safety of dental amalgam. The main point of contention is whether the mercury contained in dental restorations is released in sufficient quantities to pose a public health hazard. Most scientists agree that current evidence is not sufficient to show that amalgam restorations pose a general health threat. But researchers on both sides of this debate agree that much remains to be learned about any potential toxicity of dental amalgam-an issue serious enough to merit additional research. RP BROWN, LJ (reprint author), NATL INST DENTAL RES,EPIDEMIOL & DIS PREVENT PROGRAM,BETHESDA,MD 20892, USA. NR 53 TC 11 Z9 11 U1 0 U2 0 PU AMER DENTAL ASSN PI CHICAGO PA 211 E CHICAGO AVE, CHICAGO, IL 60611 SN 0002-8177 J9 J AM DENT ASSOC JI J. Am. Dent. Assoc. PD AUG PY 1991 VL 122 IS 9 BP 73 EP 77 PG 5 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA GA277 UT WOS:A1991GA27700016 PM 1918688 ER PT J AU HARRIS, TB MAKUC, DM KLEINMAN, JC GILLUM, RF CURB, JD SCHATZKIN, A FELDMAN, JJ AF HARRIS, TB MAKUC, DM KLEINMAN, JC GILLUM, RF CURB, JD SCHATZKIN, A FELDMAN, JJ TI IS THE SERUM CHOLESTEROL-CORONARY HEART-DISEASE RELATIONSHIP MODIFIED BY ACTIVITY LEVEL IN OLDER PERSONS SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article ID FACTOR INTERVENTION TRIAL; HIGH BLOOD CHOLESTEROL; RISK FACTOR; FOLLOW-UP; MYOCARDIAL-INFARCTION; MORTALITY; MEN; DEATH; ADULTS; LIPIDS AB Although coronary heart disease remains a leading cause of death and disability in old age, the relationship of serum cholesterol level to risk of coronary heart disease in old age is controversial. Data for 2,388 white persons aged 65-74 who participated in the National Health and Nutrition Examination Survey (NHANES) I Epidemiologic Follow-up Study (NHEFS) were examined to determine the relationship of serum cholesterol level to coronary heart disease incidence and whether activity level would modify this relationship. While there was no overall relationship between serum cholesterol level and coronary heart disease risk in either men or women, the relationship between serum cholesterol level and coronary heart disease differed within activity groups. For persons who were more active, serum cholesterol level was associated with a graded increase in risk of coronary heart disease, from 1.3 (95% CI 0.7, 2.3) in those with serum cholesterol level of 4.7-5.1 to 1.7 in those with serum cholesterol level of 6.2 mmol/L or more (95% CI 1.0, 2.7), when compared with those with serum cholesterol level below 4.7. For the least active persons, all levels of cholesterol were associated with a significant inverse relative risk, including cholesterol of 6.2 mmol/L or more (Relative risk = 0.4 (95% CI 0.2, 0.7)). These data suggest that factors such as activity level may modify the serum cholesterol-coronary heart disease association in old age. The serum cholesterol-coronary heart disease association in more active older persons resembles that seen in younger populations, whereas the association in less active persons is that of serum cholesterol level and risk of cancer or death. The modification of the serum cholesterol-coronary heart disease association by activity level may have implications for appropriate clinical management as well as appropriate design of research studies of this association. C1 UNIV HAWAII,JOHN A BURNS SCH MED,HONOLULU,HI 96822. NCI,DIV CANC PREVENT & CONTROL,BETHESDA,MD 20892. RP HARRIS, TB (reprint author), NATL CTR HLTH STAT,OFF ANAL,6525 BELCREST RD,ROOM 1000,HYATTSVILLE,MD 20782, USA. NR 38 TC 22 Z9 22 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD AUG PY 1991 VL 39 IS 8 BP 747 EP 754 PG 8 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA GA273 UT WOS:A1991GA27300001 PM 2071804 ER PT J AU SALERNO, JA HAXBY, JV GRADY, CL GONZALEZAVILES, A RAPOPORT, SI SCHAPIRO, MB AF SALERNO, JA HAXBY, JV GRADY, CL GONZALEZAVILES, A RAPOPORT, SI SCHAPIRO, MB TI CORRELATION OF CHANGE IN CEREBRAL GLUCOSE-UTILIZATION WITH COGNITIVE DECLINE IN DEMENTIA OF THE ALZHEIMER TYPE (DAT) - A LONGITUDINAL-STUDY SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract C1 NIA,NEUROSCI LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD AUG PY 1991 VL 39 IS 8 BP A8 EP A8 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA GA273 UT WOS:A1991GA27300057 ER PT J AU FLESSNER, MF AF FLESSNER, MF TI PERITONEAL TRANSPORT PHYSIOLOGY - INSIGHTS FROM BASIC RESEARCH SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Review DE PERITONEUM; DIALYSIS; INTERSTITIUM; CAPILLARY; MESOTHELIUM; DIFFUSION; CONVECTION; LYMPHATICS ID DISTRIBUTED MODEL; SKELETAL-MUSCLE; INTERSTITIAL FLUID; PLASMA TRANSPORT; NEOPLASTIC TISSUES; RAT; PERMEABILITY; PROTEIN; MESENTERY; DIALYSIS AB Clinical uses of the peritoneal cavity, such as i.p. chemotherapy or peritoneal dialysis, depend on underlying physiological mechanisms of transport between the blood and the peritoneal cavity. Clinical models of peritoneal transport have focused on an idealized "peritoneal membrane." However, such a membrane does not physically exist. Transport actually occurs between the peritoneal cavity and blood which is contained in discrete capillaries distributed in the tissue interstitium surrounding the cavity. To integrate the properties of the capillaries and the interstitium, the "distributed model" approach couples pore theory, which simulates transendothelial transport, with diffusion and convection within the tissue space. The distributed theory can explain why the peritoneal membrane, when compared with the artificial kidney, appears tight to urea but leaky to protein. The additional resistance to urea transport has been attributed to "unstirred layers" adjacent to the peritoneal membrane. These can now be defined physiologically by examining diffusion in the tissue space. Absolute rates of convection into and out of the cavity cannot yet be accurately predicted, but the physiological forces can be specified. Net "ultrafiltration" during dialysis results from not only high osmotic pressure in the peritoneal dialysate but also from a small but significant hydrostatic pressure which drives convection in the opposite direction. Recent implications from protein absorption studies that lymphatics are the cause of the decrease in net ultrafiltration are only partly true. Analysis of data from the tissue space has shown that the deposition of protein occurs from the cavity into the tissue interstitium with a slow uptake into lymphatics. RP FLESSNER, MF (reprint author), NIH,KIDNEY & ELECTROLYTE METAB LAB,BLDG 10,ROOM 6N-307,BETHESDA,MD 20892, USA. NR 87 TC 110 Z9 110 U1 1 U2 5 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD AUG PY 1991 VL 2 IS 2 BP 122 EP 135 PG 14 WC Urology & Nephrology SC Urology & Nephrology GA GB714 UT WOS:A1991GB71400002 PM 1954325 ER PT J AU PREMKUMAR, A SHAWKER, TH CHOYKE, PL AF PREMKUMAR, A SHAWKER, TH CHOYKE, PL TI SONOGRAPHIC DIAGNOSIS OF TESTICULAR SEMINOMA OCCURRING 8 YEARS AFTER INITIAL EXTRAGONADAL SEMINOMA SO JOURNAL OF ULTRASOUND IN MEDICINE LA English DT Article ID NEOPLASMS; TESTIS; TUMORS; US C1 GEORGETOWN UNIV,MED CTR,DEPT RADIOL,WASHINGTON,DC 20007. RP PREMKUMAR, A (reprint author), NIH,WARREN G MAGNUSON CLIN CTR,DEPT DIAGNOST RADIOL,BLDG 10,ROOM 1C660,BETHESDA,MD 20892, USA. NR 7 TC 3 Z9 3 U1 0 U2 0 PU AMER INST ULTRASOUND MEDICINE PI LAUREL PA SUBSCRIPTION DEPT, 14750 SWEITZER LANE, STE 100, LAUREL, MD 20707-5906 SN 0278-4297 J9 J ULTRAS MED JI J. Ultrasound Med. PD AUG PY 1991 VL 10 IS 8 BP 469 EP 471 PG 3 WC Acoustics; Radiology, Nuclear Medicine & Medical Imaging SC Acoustics; Radiology, Nuclear Medicine & Medical Imaging GA FY182 UT WOS:A1991FY18200016 PM 1942238 ER PT J AU MICKISCH, GH MERLINO, GT AIKEN, PM GOTTESMAN, MM PASTAN, I AF MICKISCH, GH MERLINO, GT AIKEN, PM GOTTESMAN, MM PASTAN, I TI NEW POTENT VERAPAMIL DERIVATIVES THAT REVERSE MULTIDRUG RESISTANCE IN HUMAN RENAL-CARCINOMA CELLS AND IN TRANSGENIC MICE EXPRESSING THE HUMAN MDR1 GENE SO JOURNAL OF UROLOGY LA English DT Article DE NEOPLASMS; GENETICS; VERAPAMIL; DRUG THERAPY ID INTRINSIC DRUG-RESISTANCE; P-GLYCOPROTEIN; MECHANISMS; THERAPY; CANCER; RNA AB Multidrug resistance in human renal cell carcinoma is mainly caused by expression of the MDR1 gene and is characterized by a broad spectrum cross resistance to many natural product chemotherapeutic agents. This resistance can be overcome by applying chemosensitizers which inhibit the function of the MDR1 gene product P-glycoprotein. The development of new reversing agents with fewer side effects and a higher potency in modifying resistance is a high priority of research on drug resistance. We have evaluated four new verapamil derivatives on 21 primary human renal cell carcinomas in vitro, and also tested them in an MDR-transgenic mice model. These mice express the human MDR1 gene in their bone marrow cells and measurement of their white blood counts provides a simple, rapid and reliable system to screen for the potency of MDR-reversing agents in vivo. We demonstrate here that all four drugs are effective in reversing multidrug resistance in primary cultures of human renal cell carcinomas when used in combination with vinblastine chemotherapy, and to a lesser extent with doxorubicin or daunomycin chemotherapy. Our in vivo data indicate that two of these reversing agents display low toxicity at high concentrations and are more effective at low, clinically achievable concentrations, than the other two drugs and R-verapamil. These results make the two new drugs attractive candidates to be taken into clinical trials. C1 NCI,DCBDC,MOLEC BIOL LAB,900 ROCKVILLE PIKE,BETHESDA,MD 20892. NCI,DCBDC,CELL BIOL LAB,BETHESDA,MD 20892. UNIV HEIDELBERG,MANNHEIM HOSP,DEPT UROL,W-6900 HEIDELBERG,GERMANY. NR 20 TC 36 Z9 36 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-5347 J9 J UROLOGY JI J. Urol. PD AUG PY 1991 VL 146 IS 2 BP 447 EP 453 PG 7 WC Urology & Nephrology SC Urology & Nephrology GA FZ333 UT WOS:A1991FZ33300049 PM 1677434 ER PT J AU NEWMAN, KD NGUYEN, N DICHEK, DA AF NEWMAN, KD NGUYEN, N DICHEK, DA TI QUANTIFICATION OF VASCULAR GRAFT SEEDING BY USE OF COMPUTER-ASSISTED IMAGE-ANALYSIS AND GENETICALLY MODIFIED ENDOTHELIAL-CELLS SO JOURNAL OF VASCULAR SURGERY LA English DT Article ID MEDIATED GENE-TRANSFER; IMPLANTATION; EXPRESSION; RETROVIRUS; LINEAGE AB Current methods for the evaluation of retention of endothelial cells seeded on vascular grafts are limited by the inability to specifically identify and quantitate seeded cells on a long-term basis. To address this problem we developed a method of quantification of graft surface coverage using genetic labeling of endothelial cells combined with computer-assisted image analysis. Rabbit aortic endothelial cells were transduced with a marker gene (lac-Z) and seeded on polytetrafluoroethylene grafts. After histochemical staining in which the genetically labeled cells turn blue, computer-assisted image analysis was used to measure the percentage of graft surface covered by the seeded cells. The utility of the method was evaluated by using it to assess the effect on graft coverage of seeded cell density and by precoating with fibronectin. Quantification of surface area coverage was automated and reproducible both between scans and between observers. Use of this method allowed the determination of a linear correlation between cell density in the seeding suspension and graft coverage (r2 = 0.93, p < 0.0001). The method also permitted confirmation of the positive contribution of fibronectin coating to graft coverage by seeded cells: 73% coverage coated versus 8% coverage uncoated (p < 0.0001). The ability of this method to specifically identify genetically marked endothelial cells and their progeny makes it attractive for use in studies targeted at optimization of graft coverage in vivo. C1 NHLBI,MOLEC HEMATOL BRANCH,BLDG 10,ROOM 7D-18,BETHESDA,MD 20892. NR 28 TC 16 Z9 16 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0741-5214 J9 J VASC SURG JI J. Vasc. Surg. PD AUG PY 1991 VL 14 IS 2 BP 140 EP 146 PG 7 WC Surgery; Peripheral Vascular Disease SC Surgery; Cardiovascular System & Cardiology GA GA522 UT WOS:A1991GA52200002 PM 1861324 ER PT J AU LITTAUA, RA OLDSTONE, MBA TAKEDA, A DEBOUCK, C WONG, JT TUAZON, CU MOSS, B KIEVITS, F ENNIS, FA AF LITTAUA, RA OLDSTONE, MBA TAKEDA, A DEBOUCK, C WONG, JT TUAZON, CU MOSS, B KIEVITS, F ENNIS, FA TI AN HLA-C-RESTRICTED CD8+ CYTOTOXIC LYMPHOCYTE-T CLONE RECOGNIZES A HIGHLY CONSERVED EPITOPE ON HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GAG SO JOURNAL OF VIROLOGY LA English DT Article ID RECOMBINANT VACCINIA VIRUS; HLA-CW3 TRANSGENIC MICE; SEROPOSITIVE INDIVIDUALS; CHORIOMENINGITIS VIRUS; SYNTHETIC PEPTIDES; FINE SPECIFICITY; HIV INFECTION; CELL RESPONSE; AIDS VIRUS; HTLV-III AB A unique epitope on the gag protein of human immunodeficiency virus type 1 (HIV-1), located at amino acid 145 to 150, has been mapped by using a CD8+ cytotoxic T-lymphocyte (CTL) clone. This epitope is highly conserved among 18 HIV-1 strains. The HIV-1 gag-specific human leukocyte antigen (HLA) class I-restricted CD8+ CTL clone was generated from fresh peripheral blood mononuclear cells of an HIV-seropositive donor by stimulation with gamma-irradiated allogeneic peripheral blood mononuclear cells in the presence of an anti-CD3 monoclonal antibody and recombinant interleukin-2. This gag-specific CTL clone killed autologous target cells infected with a recombinant vaccinia virus containing the gag gene of HIV-1 and target cells pulsed with an authentic p24gag construct expressed in Escherichia coli. Fine specificity was determined by using a panel of overlapping 30-amino-acid-long synthetic peptides and subsequently using smaller peptides to precisely map the CTL domain on p24. The epitope is on a highly conserved region, and it overlaps with a major B-cell epitope of gag. This CD8+ T-cell epitope is restricted by HLA-Cw3, which has not been previously identified as a restricting element for human CTL responses. C1 UNIV MASSACHUSETTS, MED CTR, DEPT MED, DIV INFECT DIS & IMMUNOL, WORCESTER, MA 01655 USA. Scripps Res Inst, RES INST, DEPT NEUROPHARMACOL, LA JOLLA, CA 92037 USA. SMITH KLINE BEECHAM PHARMACEUT, DEPT MOLEC GENET, KING OF PRUSSIA, PA 19406 USA. HARVARD UNIV, SCH MED, IMMUNOL ALLERGY & IMMUNOPATHOL UNIT, BOSTON, MA 02114 USA. GEORGE WASHINGTON UNIV, MED CTR, DEPT MED, DIV INFECT DIS, WASHINGTON, DC 20037 USA. NIAID, VIRAL DIS LAB, BETHESDA, MD 20892 USA. UNIV AMSTERDAM, DEPT IMMUNOHEMATOL, 1060 CX AMSTERDAM, NETHERLANDS. UNIV AMSTERDAM, EXPTL & CLIN IMMUNOL LAB, 1060 CX AMSTERDAM, NETHERLANDS. FU NIAID NIH HHS [T32-AI07272, UO1-AI26458, R01-AI24750] NR 52 TC 96 Z9 97 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 1991 VL 65 IS 8 BP 4051 EP 4056 PG 6 WC Virology SC Virology GA FY297 UT WOS:A1991FY29700010 PM 1712857 ER PT J AU PRIEL, E SHOWALTER, SD ROBERTS, M OROSZLAN, S BLAIR, DG AF PRIEL, E SHOWALTER, SD ROBERTS, M OROSZLAN, S BLAIR, DG TI THE TOPOISOMERASE-I INHIBITOR, CAMPTOTHECIN, INHIBITS EQUINE INFECTIOUS-ANEMIA VIRUS-REPLICATION IN CHRONICALLY INFECTED CF2TH CELLS SO JOURNAL OF VIROLOGY LA English DT Article ID DNA; MUTANTS; INVITRO; AIDS; GAG AB Camptothecin (CPT), a topoisomerase I-specific inhibitor, was found in this study to inhibit the replication of equine infectious anemia virus (EIAV) in chronically infected CF2Th cells (designated CF2Th/EIAV). By measuring viral reverse transcriptase activity in the culture medium, we demonstrated that treatment for 1 h with noncytotoxic doses of this drug inhibited production by 32 to 52%, whereas continuous exposure to this drug resulted in an 85 to 92% inhibition. No effect on the viability or growth rate of the cells was detected in any of these treatments. Indirect immunofluorescence analysis of the CPT-treated CF2Th/EIAV cells with anti-p26 capsid protein antibodies showed 60 to 85% reduction in the immunofluorescence-positive cells following drug treatment, and radioimmunoprecipitation analysis of these cells showed a comparable decrease of the pr55gag precursor protein. These data suggest that CPT acts as an anti-EIAV agent to block virus replication in the chronically infected cells. C1 NCI,FREDERICK CANC RES & DEV CTR,MOLEC ONCOL LAB,FREDERICK,MD 21701. PROGRAM RESOURCES INC,FREDERICK,MD 21701. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21702. FU NCI NIH HHS [N01-CO-74101] NR 26 TC 31 Z9 33 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 1991 VL 65 IS 8 BP 4137 EP 4141 PG 5 WC Virology SC Virology GA FY297 UT WOS:A1991FY29700021 PM 1649321 ER PT J AU TREANOR, JJ BUJA, R MURPHY, BR AF TREANOR, JJ BUJA, R MURPHY, BR TI INTRAGENIC SUPPRESSION OF A DELETION MUTATION OF THE NONSTRUCTURAL GENE OF AN INFLUENZA-A VIRUS SO JOURNAL OF VIROLOGY LA English DT Article ID TEMPERATURE-SENSITIVE PHENOTYPE; REASSORTANT VIRUSES; RESPIRATORY-TRACT; RNA SEGMENT-8; NS GENE; MUTANT; SEQUENCE; ATTENUATION; VIRULENCE; REVERTANT AB The influenza A/Alaska/77 (H3N2) virus mutant 143-1 is temperature sensitive (ts) due to a spontaneous in-frame 36-nucleotide deletion in the nonstructural (NS) gene segment, which leads to a 12-amino-acid deletion in the NS1 protein. In addition, it has a small-plaque phenotype on MDCK cell monolayers. However, phenotypically revertant (i.e., ts+) viruses were isolated readily following replication of the 143-1 virus both in vitro and in vivo. In order to determine the genetic mechanism by which escape from the ts phenotype occurred, we performed segregational analysis and found that an intrasegmental suppressor mutation caused the loss of the ts phenotype. Nucleotide sequence analysis revealed the presence of an intragenic mutation in each of the ts+ phenotypic revertant viruses, involving a substitution of valine for alanine at amino acid 23 of the NS1 protein. This mutation resulted in acquisition of the ts+ phenotype and also in the large-plaque phenotype on MDCK cells, characteristic of the wild-type A/Alaska/77 parent virus. This amino acid substitution is predicted to generate an area of alpha helix in the secondary structure of the amino-terminal portion of the NS1 protein of the revertant viruses which may compensate for loss of an alpha-helical region due to the deletion of amino acids 66 to 77 in the NS1 protein of the 143-1 virus. C1 NIH,INFECT DIS LAB,BETHESDA,MD 20892. RP TREANOR, JJ (reprint author), UNIV ROCHESTER,INFECT DIS UNIT,601 ELMWOOD AVE,BOX 689,ROCHESTER,NY 14642, USA. NR 29 TC 11 Z9 11 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 1991 VL 65 IS 8 BP 4204 EP 4210 PG 7 WC Virology SC Virology GA FY297 UT WOS:A1991FY29700030 PM 1830112 ER PT J AU CHATTOPADHYAY, SK SENGUPTA, DN FREDRICKSON, TN MORSE, HC HARTLEY, JW AF CHATTOPADHYAY, SK SENGUPTA, DN FREDRICKSON, TN MORSE, HC HARTLEY, JW TI CHARACTERISTICS AND CONTRIBUTIONS OF DEFECTIVE, ECOTROPIC, AND MINK CELL FOCUS-INDUCING VIRUSES INVOLVED IN A RETROVIRUS-INDUCED IMMUNODEFICIENCY SYNDROME OF MICE SO JOURNAL OF VIROLOGY LA English DT Article ID MURINE LEUKEMIA VIRUSES; FORMING VIRUSES; MAIDS; INDUCTION; DISEASE; SUSCEPTIBILITY; LYMPHOMAS; STRAINS; DNA AB LP-BM5 murine leukemia virus, a derivative of Duplan-Laterjet virus, contains a mixture of replication-competent B-tropic ecotropic and mink cell focus-inducing (MCF) viruses and a defective genome that is the proximal cause of a syndrome, murine AIDS (MAIDS), characterized by lymphoproliferation and immunodeficiency. The defective (BM5d) and ecotropic components of this mixture were molecularly cloned, and complete (BM5d) or partial (ecotropic) nucleotide sequences were determined. BM5d closely resembled the Du5H genome cloned from the Duplan virus, featuring a highly divergent p12 sequence in the gag open reading frame. In MAIDS-sensitive C57BL/6 mice, BM5d was detected in tissues within 2 weeks of infection but was absent from tissues of the MAIDS-resistant strain, A/J, 12 weeks after infection. B-cell-lineage tumors from mice with MAIDS contained and expressed BM5d, and clonal integrations of this genome were variably associated with clonal expansions of B cells in infected mice. Finally, mRNA crosshybridizing with a probe for BM5d was present in spleen but not kidney cells of uninfected B6 mice. C1 UNIFORMED SERV UNIV HLTH SCI,DEPT PATHOL,BETHESDA,MD 20814. UNIV CONNECTICUT,DEPT PATHOBIOL,STORRS,CT 06268. RP CHATTOPADHYAY, SK (reprint author), NIAID,IMMUNOPATHOL LAB,BETHESDA,MD 20892, USA. OI Morse, Herbert/0000-0002-9331-3705 FU NIAID NIH HHS [N01-AI-72622] NR 30 TC 126 Z9 128 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 1991 VL 65 IS 8 BP 4232 EP 4241 PG 10 WC Virology SC Virology GA FY297 UT WOS:A1991FY29700034 PM 1649328 ER PT J AU ROSS, EK BUCKLERWHITE, AJ RABSON, AB ENGLUND, G MARTIN, MA AF ROSS, EK BUCKLERWHITE, AJ RABSON, AB ENGLUND, G MARTIN, MA TI CONTRIBUTION OF NF-KAPPA-B AND SP1 BINDING MOTIFS TO THE REPLICATIVE CAPACITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 - DISTINCT PATTERNS OF VIRAL GROWTH ARE DETERMINED BY T-CELL TYPES SO JOURNAL OF VIROLOGY LA English DT Article ID LONG TERMINAL REPEAT; TRANSCRIPTIONAL REGULATION; TRANS-ACTIVATOR; SV40 ENHANCER; HIV-1 LTR; HTLV-III; PROMOTER; ELEMENTS; SEQUENCES; REGION AB Starting with a replication-incompetent molecular clone of human immunodeficiency virus type 1, lacking all the NF-kB and Sp1 binding sites present in the native long terminal repeat (LTR), proviruses containing reconstructed LTRs with individual or combinations of NF-kB and Sp1 elements were generated and evaluated for their capacity to produce virus progeny following transfection-cocultivation. Virus stocks obtained from these experiments exhibited a continuum of replicative capacities in different human T-cell types depending on which element(s) was present in the LTR. For example, in experiments involving proviral clones with LTRs containing one or two NF-kappa-B elements (and no Sp1 binding sites), a hierarchy of cellular permissivity to virus replication (peripheral blood lymphocytes = MT4 > H9 > CEM > Jurkat) was observed. Of note was the associated emergence of second-site LTR revertants which involved an alteration of the TATA box. These results suggest that the human immunodeficiency virus type 1 LTR possesses functional redundancy which ensures virus replication in different T-cell types and is capable of changing depending on the particular combination of transcriptional factors present. C1 NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. GEORGETOWN UNIV,SCH MED,DIV MOLEC VIROL & IMMUNOL,ROCKVILLE,MD 20854. NR 47 TC 166 Z9 167 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 1991 VL 65 IS 8 BP 4350 EP 4358 PG 9 WC Virology SC Virology GA FY297 UT WOS:A1991FY29700048 PM 2072454 ER PT J AU GOTTSCHALCK, E ALEXANDERSEN, S COHN, A POULSEN, LA BLOOM, ME AASTED, B AF GOTTSCHALCK, E ALEXANDERSEN, S COHN, A POULSEN, LA BLOOM, ME AASTED, B TI NUCLEOTIDE-SEQUENCE ANALYSIS OF ALEUTIAN MINK DISEASE PARVOVIRUS SHOWS THAT MULTIPLE VIRUS TYPES ARE PRESENT IN INFECTED MINK SO JOURNAL OF VIROLOGY LA English DT Article ID ACUTE INTERSTITIAL PNEUMONIA; FELINE PANLEUKOPENIA VIRUS; MINUTE VIRUS; CANINE PARVOVIRUS; AUTONOMOUS PARVOVIRUS; VIRAL REPLICATION; IMMUNE-RESPONSE; DNA-SEQUENCE; CELL-CULTURE; PROTEIN GENE AB Different isolates of Aleutian mink disease parvovirus (ADV) were cloned and nucleotide sequenced. Analysis of individual clones from two in vivo-derived isolates of high virulence indicated that more than one type of ADV DNA were present in each of these isolates. Analysis of several clones from two preparations of a cell culture-adapted isolate of low virulence showed the presence of only one type of ADV DNA. We also describe the nucleotide sequence from map units 44 to 88 of a new type of ADV DNA. The new type of ADV DNA is compared with the previously published ADV sequences, to which it shows 95% homology. These findings indicate that ADV, a single-stranded DNA virus, has a considerable degree of variability and that several virus types can be present simultaneously in an infected animal. C1 IOWA STATE UNIV SCI & TECHNOL,COLL VET MED,DEPT VET MICROBIOL & PREVENT MED,AMES,IA 50011. ROYAL VET & AGR UNIV,DEPT VET MICROBIOL,VIROL & IMMUNOL LAB,DK-1870 FREDERIKSBERG C,DENMARK. NIAID,PERSISTANT VIRAL DIS LAB,ROCKY MTN LABS,HAMILTON,MT 59840. OI Alexandersen, Soren/0000-0002-5039-3178 NR 49 TC 47 Z9 50 U1 0 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 1991 VL 65 IS 8 BP 4378 EP 4386 PG 9 WC Virology SC Virology GA FY297 UT WOS:A1991FY29700051 PM 1649336 ER PT J AU MARCHETTI, A ROBBINS, J CAMPBELL, G BUTTITTA, F SQUARTINI, F BISTOCCHI, M CALLAHAN, R AF MARCHETTI, A ROBBINS, J CAMPBELL, G BUTTITTA, F SQUARTINI, F BISTOCCHI, M CALLAHAN, R TI HOST GENETIC BACKGROUND EFFECT ON THE FREQUENCY OF MOUSE MAMMARY-TUMOR VIRUS-INDUCED REARRANGEMENTS OF THE INT-1 AND INT-2 LOCI IN MOUSE MAMMARY-TUMORS SO JOURNAL OF VIROLOGY LA English DT Note ID PROTO-ONCOGENES; TRANSGENIC MICE; GROWTH-FACTORS; CELLULAR GENE; EXPRESSION; ACTIVATION; REGION; TUMORIGENESIS; PROTOONCOGENE; TRANSMISSION AB The frequency with which int-1 and int-2 are rearranged in mouse mammary tumors by mouse mammary tumor virus (MMTV)-induced insertional mutagenesis is a consequence of the host genetic background. In 75% of C3H mammary tumors, int-1 is rearranged by MMTV insertion, whereas only 30% of BALB/cfC3H tumors contain a virus-induced rearrangement of int-1. This difference is significant (P < 0.005) and could not be accounted for by the potentially additive effect of the genetically transmitted Mtv-1-encoded virus in C3H mice. Similarly, MMTV-induced rearrangement of the int-2 gene in mammary tumors of the R111 mouse strain (59%) occurred at a significantly (P < 0.025) higher frequency than in BALB/cfR111 (25%) mammary tumors. Moreover, in BALB/cfR111 mammary tumors, there is evidence that rearrangement of int-1 and int-2 does not occur independently (P < 0.025). These results suggest that the long history of inbreeding for high tumor incidence of C3H and R111 mouse strains has selected for the fixation of host mutations which either complement the action of the particular int gene or affect the sensitivity of specific subpopulations of mammary epithelium to infection by particular strains of MMTV. C1 NCI,TUMOR IMMUNOL & BIOL LAB,BETHESDA,MD 20892. NCI,DIV COMP RES & TECHNOL,STAT & MATH METHODOL LAB,BETHESDA,MD 20892. UNIV PISA,INST PATHOL ANAT & HISTOL,I-56100 PISA,ITALY. NR 29 TC 23 Z9 23 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 1991 VL 65 IS 8 BP 4550 EP 4554 PG 5 WC Virology SC Virology GA FY297 UT WOS:A1991FY29700078 PM 1712864 ER PT J AU NOVAK, MA BAYNE, K AF NOVAK, MA BAYNE, K TI MONKEY BEHAVIOR AND LABORATORY ISSUES - INTRODUCTION SO LABORATORY ANIMAL SCIENCE LA English DT Editorial Material C1 NIH,NATL CTR RES RESOURCES,OFF DIRECTOR,OFF ANIM CARE & USE,BETHESDA,MD 20892. RP NOVAK, MA (reprint author), UNIV MASSACHUSETTS,DEPT PSYCHOL,AMHERST,MA 01003, USA. NR 0 TC 5 Z9 5 U1 0 U2 0 PU AMER ASSOC LABORATORY ANIMAL SCIENCE PI CORDOVA PA 70 TIMBERCREEK DR, SUITE 5, CORDOVA, TN 38018 SN 0023-6764 J9 LAB ANIM SCI JI Lab. Anim. Sci. PD AUG PY 1991 VL 41 IS 4 BP 306 EP 307 PG 2 WC Veterinary Sciences; Zoology SC Veterinary Sciences; Zoology GA GK683 UT WOS:A1991GK68300001 ER PT J AU NOVAK, MA SUOMI, SJ AF NOVAK, MA SUOMI, SJ TI SOCIAL-INTERACTION IN NONHUMAN-PRIMATES - AN UNDERLYING THEME FOR PRIMATE RESEARCH SO LABORATORY ANIMAL SCIENCE LA English DT Article ID RHESUS-MONKEYS; MACACA-MULATTA; SQUIRREL-MONKEY; BREEDING COLONY; MACAQUES; BEHAVIOR; EXPERIENCE; MANAGEMENT; NEMESTRINA; MORTALITY AB Social living is assumed to be a critical feature of nonhuman primate existence inasmuch as most primate species live in social groups in nature. Recent USDA legislation emphasizes the importance of social contact in promoting psychological well-being and recommends that laboratory primates be housed with companions when consistent with research protocols. Our goals were to examine the link between social housing and psychological well-being and to explore the idea that research may be compromised when primates are studied in environments that vary too greatly from their natural ecological setting (individual cage housing versus group housing). Three general points emerge from these examinations. First, providing companionship may be a very potent way in which to promote psychological well-being in nonhuman primates; however, social living is not synonymous with well-being. The extent to which social housing promotes psychological well-being can vary across species and among individual members of the same species (for example, high- and low-ranking monkeys). Secondly, housing conditions can affect research outcomes in that group-housed animals may differ from individually housed animals in response to some manipulation. Social interaction may be a significant variable in regulating the biobehavioral responses of nonhuman primates to experimental manipulations. Finally, a larger number of socially housed subjects than individually housed subjects may be necessary for some biomedical research projects to yield adequate data analysis. Thus, social living has significant benefits and some potential costs not only for the animals themselves, but for the research enterprise. C1 NICHHD,COMPARAT ETHOL LAB,BETHESDA,MD 20892. RP NOVAK, MA (reprint author), UNIV MASSACHUSETTS,DEPT PSYCHOL,AMHERST,MA 01003, USA. FU NCRR NIH HHS [RR07048] NR 73 TC 36 Z9 36 U1 1 U2 7 PU AMER ASSOC LABORATORY ANIMAL SCIENCE PI CORDOVA PA 70 TIMBERCREEK DR, SUITE 5, CORDOVA, TN 38018 SN 0023-6764 J9 LAB ANIM SCI JI Lab. Anim. Sci. PD AUG PY 1991 VL 41 IS 4 BP 308 EP 314 PG 7 WC Veterinary Sciences; Zoology SC Veterinary Sciences; Zoology GA GK683 UT WOS:A1991GK68300002 PM 1658476 ER PT J AU RASMUSSEN, KLR AF RASMUSSEN, KLR TI IDENTIFICATION, CAPTURE, AND BIOTELEMETRY OF SOCIALLY LIVING MONKEYS SO LABORATORY ANIMAL SCIENCE LA English DT Article ID MACAQUE MACACA-SILENUS AB Remote monitoring of physiologic function using socially living monkeys differs from that using individually housed animals in that access to subjects may be limited. Some logistic aspects of working with socially housed monkeys are reviewed, including identification of individuals and capturing subjects. Methods of remote sampling include hormonal assays of urine and fecal samples, measurement of physical indices as estimates of reproductive status, and the use of telemetry devices to record activity and biopotentials. Key factors in the selection of a telemetry system are discussed. In many cases, remote monitoring may permit assessment of physiologic function without the stress of handling or restraint. C1 NICHHD,COMPARAT ETHOL LAB,BETHESDA,MD 20892. NR 20 TC 4 Z9 4 U1 0 U2 0 PU AMER ASSOC LABORATORY ANIMAL SCIENCE PI CORDOVA PA 70 TIMBERCREEK DR, SUITE 5, CORDOVA, TN 38018 SN 0023-6764 J9 LAB ANIM SCI JI Lab. Anim. Sci. PD AUG PY 1991 VL 41 IS 4 BP 350 EP 354 PG 5 WC Veterinary Sciences; Zoology SC Veterinary Sciences; Zoology GA GK683 UT WOS:A1991GK68300008 PM 1658482 ER PT J AU BAYNE, K AF BAYNE, K TI ALTERNATIVES TO CONTINUOUS SOCIAL HOUSING SO LABORATORY ANIMAL SCIENCE LA English DT Article ID CAPTIVE CHIMPANZEES; ENRICHMENT DEVICE; CAGE SIZE; BEHAVIOR; MONKEYS AB Although social housing is desirable for social species of nonhuman primates, circumstances arise whereby social housing is precluded (for example, certain kinds of infectious disease or toxicologic research, when the health of the animals(s) would be compromised by social housing, and animals which respond behaviorally in an inappropriate manner to social housing). Nonsocial alternatives that provide increased environmental complexity to the home cage should then be considered. Nonsocial "environmental enrichment" schemes can be designed to enhance the expression of an individually housed nonhuman primate's locomotive/postural, manipulative, and foraging behaviors. In this way, nonsocial, but species-typical, behaviors can be promoted in the single cage housing condition. RP BAYNE, K (reprint author), NIH,NATL CTR RES RESOURCES,OFF DIRECTOR,OFF ANIM CARE & USE,BETHESDA,MD 20892, USA. NR 50 TC 8 Z9 8 U1 0 U2 1 PU AMER ASSOC LABORATORY ANIMAL SCIENCE PI CORDOVA PA 70 TIMBERCREEK DR, SUITE 5, CORDOVA, TN 38018 SN 0023-6764 J9 LAB ANIM SCI JI Lab. Anim. Sci. PD AUG PY 1991 VL 41 IS 4 BP 355 EP 359 PG 5 WC Veterinary Sciences; Zoology SC Veterinary Sciences; Zoology GA GK683 UT WOS:A1991GK68300009 PM 1658483 ER PT J AU ROVNAK, J CASEY, JW BOYD, AL GONDA, MA COCKERELL, GL AF ROVNAK, J CASEY, JW BOYD, AL GONDA, MA COCKERELL, GL TI ISOLATION OF BOVINE LEUKEMIA-VIRUS INFECTED ENDOTHELIAL-CELLS FROM CATTLE WITH PERSISTENT LYMPHOCYTOSIS SO LABORATORY INVESTIGATION LA English DT Article DE PSEUDOEMPERIPOLESIS; LYMPHOMA ID LYMPH-NODES; STROMAL CELLS; EXPRESSION; AIDS; IDENTIFICATION; ANTIBODIES; MORPHOLOGY; MONOCYTES; ANTIGENS; CULTURE AB Incubation of adherent cells derived from peripheral blood mononuclear cells of cattle naturally infected with bovine leukemia virus (BLV) led to the establishment of three, persistently infected, primary cell cultures. These cultures were obtained exclusively from animals exhibiting persistent lymphocytosis, and not from uninfected or infected, hematologically normal cattle. The cells contained monoclonally integrated, full length BLV provirus, indicating that each culture resulted from clonal expansion of a single cell. They expressed high levels of all BLV specific mRNAs and showed intracellular reactivity to antibodies directed to viral gag and env proteins. Viral particle morphogenesis was highly restricted as determined by low levels of reverse transcriptase activity in cell supernatants and the paucity of viral particles on the cell surface. Analysis of cellular antigenic determinants, using monoclonal antibodies to bovine leukocyte differentiation and major histocompatability complex antigens, was inconclusive. Cytochemical, morphologic, and ultrastructural analyses were consistent with endothelial cells and they exhibited the distinctive functional capacity of endothelial cells derived from specialized postcapillary venules, which constitute sites of lymphocyte extravasation. These data suggest that infection of these endothelial cells may be involved in the development of persistent lymphocytosis in BLV-infected animals. C1 CORNELL UNIV,NEW YORK STATE COLL VET MED,DEPT MICROBIOL & IMMUNOL,ITHACA,NY 14853. CORNELL UNIV,ITHACA,NY 14853. NCI FREDERICK CANC RES & DEV FACIL,DYNCORP,PROGRAM RES INC,CELL & MOLEC STRUCT LAB,FREDERICK,MD. RP ROVNAK, J (reprint author), COLORADO STATE UNIV,DEPT PATHOL,FT COLLINS,CO 80523, USA. FU NCI NIH HHS [CA 43728, N01-CO-74102] NR 45 TC 24 Z9 25 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD AUG PY 1991 VL 65 IS 2 BP 192 EP 202 PG 11 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA GB186 UT WOS:A1991GB18600008 PM 1652665 ER PT J AU STRONG, MJ GARRUTO, RM AF STRONG, MJ GARRUTO, RM TI NEURON-SPECIFIC THRESHOLDS OF ALUMINUM TOXICITY INVITRO - A COMPARATIVE-ANALYSIS OF DISSOCIATED FETAL RABBIT HIPPOCAMPAL AND MOTOR NEURON-ENRICHED CULTURES SO LABORATORY INVESTIGATION LA English DT Article DE MONOLAYER CULTURE; NEUROFILAMENTOUS INCLUSIONS ID INDUCED NEUROFIBRILLARY DEGENERATION; CYTOSKELETAL PROTEINS; CELL-CULTURE; SURVIVAL INVITRO; TANGLES; TRANSPORT; MOUSE; IMMUNOFLUORESCENCE; NEUROFILAMENTS; INTOXICATION AB Mature dissociated motor neuron-enriched and hippocampal neuron cultures derived from fetal New Zealand white rabbits were continuously exposed to 1, 10, 25, 50, or 100-mu-M AlCl3 in a chemically defined medium for 14 days. Motor neuron-enriched cultures exposed to low concentrations (1 or 10-mu-M) of AlCl3 remained viable for the entire experiment but developed perikaryal and neuritic inclusions composed of phosphorylated neurofilament. Similar inclusions developed in cultures exposed to 25 and 50-mu-M AlCl3, but motor neurons did not survive beyond 10 days exposure. The 100-mu-M AlCl3-supplemented medium induced cell death within 72 hours without development of inclusions. In contrast, hippocampal neurons exposed to 1, 10, or 25-mu-M AlCl3 developed no morphological changes or inclusions. Although hippocampal cultures exposed to 50 or 100-mu-M AlCl3 developed perinuclear and proximal neuritic inclusions of phosphorylated neurofilament after 10 days, they remained viable. These in vitro morphological observations demonstrate a 10-fold greater sensitivity of spinal motor neurons to aluminum toxicity when compared with hippocampal neurons and suggest that the earlier observations of neuron-specific thresholds of aluminum toxicity in vivo are related to unique regulatory mechanisms of neurofilament biosynthesis and catabolism within distinct neuronal cell populations. C1 UNIV WESTERN ONTARIO,DEPT CLIN NEUROL SCI,LONDON N6A 3K7,ONTARIO,CANADA. NIH,CENT NERVOUS SYST STUDIES LAB,BETHESDA,MD 20892. RI Strong, Michael/H-9689-2012 NR 64 TC 26 Z9 27 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD AUG PY 1991 VL 65 IS 2 BP 243 EP 249 PG 7 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA GB186 UT WOS:A1991GB18600013 PM 1881125 ER PT J AU RASMUSSEN, N JOHNSEN, NJ BOHR, VA AF RASMUSSEN, N JOHNSEN, NJ BOHR, VA TI OTOLOGIC SEQUELAE AFTER PNEUMOCOCCAL MENINGITIS - A SURVEY OF 164 CONSECUTIVE CASES WITH A FOLLOW-UP OF 94 SURVIVORS SO LARYNGOSCOPE LA English DT Article ID ACUTE BACTERIAL-MENINGITIS; PROGNOSTIC FACTORS; CHILDREN; FEATURES; THERAPY; IMPACT AB As part of a comprehensive study on sequelae after pneumococcal meningitis, 94 of 111 consecutive survivors were re-examined 4 to 16 years after discharge. Twenty-three patients had otological sequelae after pneumococcal meningitis. In these patients, 17 had hearing losses, 7 had tinnitus, 9 had vertigo, 13 had vestibular areflexia, 4 had loss of smell, and 3 had loss of taste. Among the patients with hearing loss, 4 were bilaterally deaf, 6 were unilaterally deaf, and 2 had mild and 5 had slight hearing losses. From correlations with extensive data from the medical records, preadmission antibiotic treatment appeared to protect from acousticovestibular damage. Purulent otitis media and otosurgical intervention did not correlate to the fatality rate or the development of sequelae. Acute purulent otitis media appeared as a concomitant manifestation, rather than the focus of pneumococcal meningitis. C1 RIGSHOSP,DEPT INFECT DIS,DK-2100 COPENHAGEN,DENMARK. NCI,DIV CANC THERAPY,BETHESDA,MD 20892. RP RASMUSSEN, N (reprint author), RIGSHOSP,DEPT EAR NOSE & THROAT,OTOPATHOL LAB,BLEGDAMSVEJ 9,DK-2100 COPENHAGEN,DENMARK. NR 18 TC 27 Z9 27 U1 0 U2 0 PU LARYNGOSCOPE CO PI ST LOUIS PA 10 S BROADWAY 14TH FLOOR, ST LOUIS, MO 63102-1741 SN 0023-852X J9 LARYNGOSCOPE JI Laryngoscope PD AUG PY 1991 VL 101 IS 8 BP 876 EP 882 PG 7 WC Medicine, Research & Experimental; Otorhinolaryngology SC Research & Experimental Medicine; Otorhinolaryngology GA GU623 UT WOS:A1991GU62300012 PM 1865737 ER PT J AU HANAWASHIMIZU, M MAENO, M SHIKATA, T AF HANAWASHIMIZU, M MAENO, M SHIKATA, T TI TRANSPLANTATION STUDIES ON HUMAN AND DUCK HEPATOCYTES IN ATHYMIC NUDE-MICE SO LIVER LA English DT Article AB For determination of the most suitable tissue for heterotopic transplantation of exogenous hepatocytes, dissociated hepatocytes or small pieces of liver tissue were transplanted into the spleen, adipose tissue and inside the capsule of the kidney of BALB/c mice. Survival of syngeneic grafts of dissociated hepatocytes was highest in the spleen and that of pieces of liver tissue in the adipose tissue, but only the latter system was suitable for xenogeneic transplantation. Histological examination showed that a total of 50% of the human or duck liver tissue implants survived in the inguinal fat pad of athymic nude mice (BALB/c-nu). Histochemical analyses revealed that most hepatocytes transplanted into the fat pad gave positive reactions for glucose-6-phosphatase and with periodic acid-Schiff reagent at least 28 days after transplantation. Electron microscopic observation showed that these cells also maintained characteristic cellular organelles. This xenogeneic transplantation into adipose tissue should be useful in the studies on replication and infection of human hepatotropic viruses such as hepatitis B and C viruses. C1 NCI,FCRDC,BLDG 560,RM 31 76,FREDERICK,MD 21701. NR 0 TC 1 Z9 1 U1 0 U2 0 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0106-9543 J9 LIVER JI Liver PD AUG PY 1991 VL 11 IS 4 BP 241 EP 247 PG 7 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA FX843 UT WOS:A1991FX84300008 PM 1943505 ER PT J AU ENDO, H AKAHOSHI, T TAKAGISHI, K KASHIWAZAKI, S MATSUSHIMA, K AF ENDO, H AKAHOSHI, T TAKAGISHI, K KASHIWAZAKI, S MATSUSHIMA, K TI ELEVATION OF INTERLEUKIN-8 (IL-8) LEVELS IN JOINT FLUIDS OF PATIENTS WITH RHEUMATOID-ARTHRITIS AND THE INDUCTION BY IL-8 OF LEUKOCYTE INFILTRATION AND SYNOVITIS IN RABBIT JOINTS SO LYMPHOKINE AND CYTOKINE RESEARCH LA English DT Article ID NEUTROPHIL CHEMOTACTIC FACTOR; DEGRADATION AB Increased amounts of interleukin-8 (IL-8) were detected in synovial fluids of patients with active rheumatoid arthritis (RA) by radioimmunoassay (RIA). The concentration of IL-8 correlated directly with the number of infiltrating neutrophils in synovial fluids. To elucidate the role of IL-8 in neutrophil accumulation at the site of synovitis, the in vivo effects of intraarticular injection of recombinant IL-8 (rIL-8) on leukocytes infiltration into the joint space and synovium were examined. Following a single injection of rIL-8 into the knee joint space of rabbits, redness of the joint and limp became apparent after 4 h and were associated with the rapid infiltration of neutrophilic leukocytes into the joint space and synovial tissues. These effects were time dependent, first becoming evident at 1 h and reaching a plateau in 4 h, and also dose dependent, with a minimal effect being elicited by 100 ng per joint. Although neutrophils were present in the greatest number at 4 h, subsequently mononuclear cells accumulated and became apparent in considerable number after 8 h. Synovial lining cells became ovoid, pleomorphic, and multilayered at 24 h. IL-8 had no effect on the breakdown of proteoglycan of articular cartilage. Based on these findings, IL-8 released from monocytes and synovial cells may be an important contributor to leukocyte accumulation and inflammatory events in the joints of RA. C1 TOKYO WOMENS MED COLL,INST RHEUMATOL,SHINJU KU,TOKYO 163,JAPAN. NCI,MOLEC IMMUNOREGULAT BIOL RESPONSE MODIFIERS LAB,FREDERICK,MD 21701. KITASATO UNIV,SCH MED,DEPT ORTHOPED,SAGAMIHARA,KANAGAWA 228,JAPAN. RP ENDO, H (reprint author), KITASATO UNIV,SCH MED,DEPT INTERNAL MED,1-15-1 KITASATO,SAGAMIHARA,KANAGAWA 228,JAPAN. NR 25 TC 146 Z9 147 U1 0 U2 1 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0277-6766 J9 LYMPHOKINE CYTOK RES JI Lymphokine Cytokine Res. PD AUG PY 1991 VL 10 IS 4 BP 245 EP 252 PG 8 WC Biochemistry & Molecular Biology; Immunology SC Biochemistry & Molecular Biology; Immunology GA GD560 UT WOS:A1991GD56000002 PM 1932367 ER PT J AU FARRAR, WL WILLETTEBROWN, J LINNEKIN, D AF FARRAR, WL WILLETTEBROWN, J LINNEKIN, D TI REGULATION OF C-MYC AND ORNITHINE DECARBOXYLASE EXPRESSION BY DISTINCT PROTEIN-KINASE SYSTEMS IN IL-3-DEPENDENT MYELOID CELLS SO LYMPHOKINE AND CYTOKINE RESEARCH LA English DT Article ID TYROSINE PHOSPHORYLATION; GROWTH-FACTOR; INTERLEUKIN-2 RECEPTOR; SIGNAL TRANSDUCTION; GLUCOSE-TRANSPORT; LINE; PROLIFERATION; ACTIVATION; FDC-P1; BIOSYNTHESIS AB We have investigated whether PK-C-regulated events are independent of those biochemical events related to IL-3-induced tyrosine kinase activation by 32Dcl cells. The depletion of functional PK-C isoform activity by prolonged PMA treatment reduced the proliferative response to IL-3 by half that of untreated control cells. PK-C-deficient 32Dcl cells were unable to respond to PMA for the induction of c-myc and ODC mRNA accumulation. PK-C down-regulation did not affect IL-3-induced tyrosine phosphorylation and inhibited IL-3-regulated c-myc and ODC mRNA expression by only 30%. However, PK-C down-regulation had a pronounced inhibitory effect on IL-3 regulation of ODC enzymatic activity. While a PK-C-dependent and -independent pathway for the regulation of c-myc and ODC mRNA expression could be demonstrated, the regulation of ODC enzymatic activity appeared to require an intact PK-C system. The data suggest that the optimum biological and biochemical responses to IL-3 requires both pathways intact, however, tyrosine kinase activation and significant increases in gene products associated with proliferation can be achieved in the absence of a functional PK-C system. C1 PROGRAM RESOURCES INC,FREDERICK CANC RES & DEV CTR,BIOL CARCINOGENESIS DEV PROGRAM,FREDERICK,MD 21702. RP FARRAR, WL (reprint author), NCI,FREDERICK CANC RES & DEV CTR,CYTOKINE MOLEC MECHANISMS SECT,FREDERICK,MD 21701, USA. FU NCI NIH HHS [N01-CO-74102] NR 35 TC 2 Z9 2 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0277-6766 J9 LYMPHOKINE CYTOK RES JI Lymphokine Cytokine Res. PD AUG PY 1991 VL 10 IS 4 BP 291 EP 299 PG 9 WC Biochemistry & Molecular Biology; Immunology SC Biochemistry & Molecular Biology; Immunology GA GD560 UT WOS:A1991GD56000009 PM 1718453 ER PT J AU HIRAYOSHI, K KUDO, H TAKECHI, H NAKAI, A IWAMATSU, A YAMADA, KM NAGATA, K AF HIRAYOSHI, K KUDO, H TAKECHI, H NAKAI, A IWAMATSU, A YAMADA, KM NAGATA, K TI HSP47 - A TISSUE-SPECIFIC, TRANSFORMATION-SENSITIVE, COLLAGEN-BINDING HEAT-SHOCK PROTEIN OF CHICKEN-EMBRYO FIBROBLASTS SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID LUMINAL ER PROTEINS; ENDOPLASMIC-RETICULUM; HUMAN ALPHA-1-ANTITRYPSIN; MEMBRANE-PROTEINS; SURFACE PROTEIN; MESSENGER-RNA; ACID; SEQUENCE; HOMOLOGY; SIGNAL AB We report the isolation and characterization of a cDNA clone encoding HSP47, a transformation-sensitive heat shock protein that binds to collagen. A cDNA library was prepared from total RNA isolated from heat-shocked chicken embryo fibroblasts and screened by using oligonucleotide mixtures prepared on the basis of the N-terminal amino acid sequence of biochemically purified HSP47. The cDNA insert contained 3,278 bp, which encoded a 15-amino-acid signal peptide and a mature protein coding region consisting of 390 amino acid residues; it also included part of the 5' noncoding region and a long 3' noncoding region. The deduced amino acid sequence revealed an RDEL sequence at the C terminus, which is a variant of the KDEL retention signal for retention of proteins in the endoplasmic reticulum. Northern (RNA) blot analyses and nuclear run-on assays established that the induction of HSP47 by heat shock and its suppression after transformation of chicken embryo fibroblasts by Rous sarcoma virus are regulated at the transcriptional level. A homology search revealed that this protein belongs to the serpin family, the superfamily of plasma serine protease inhibitors. Although structurally homologous to the serpins, HSP47 lacks the active site thought to be essential for the inhibition of proteases and does not appear to bind to intracellular proteases. HSP47 is the first heat shock protein found to be a member of the serpin superfamily. Conversly, it is the first serpin family member that is not secreted from cells, which could be explained by acquisition of the RDEL retention signal during evolution. C1 KYOTO UNIV,CHEST DIS RES INST,DEPT CELL BIOL,KYOTO 60601,JAPAN. KIRIN BREWERY CO LTD,KEY TECHNOL LABS,MAEBASHI 371,JAPAN. NIDR,DEV BIOL LAB,BETHESDA,MD 20892. OI Yamada, Kenneth/0000-0003-1512-6805 NR 48 TC 104 Z9 107 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD AUG PY 1991 VL 11 IS 8 BP 4036 EP 4044 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FX723 UT WOS:A1991FX72300024 PM 2072906 ER PT J AU WEISSMAN, JD SINGER, DS AF WEISSMAN, JD SINGER, DS TI A COMPLEX REGULATORY DNA ELEMENT ASSOCIATED WITH A MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I GENE CONSISTS OF BOTH A SILENCER AND AN ENHANCER SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID INTERFERON TREATMENT; EXPRESSION; TRANSCRIPTION; BINDING; CELLS; PROMOTER; PROTEIN; ACTIVATION; ANTIGENS; SEQUENCE AB A novel regulatory element which contributes to the regulation of quantitative, tissue-specific differences in gene expression has been found between -771 and -676 bp upstream of the major histocompatibility complex (MHC) class I gene, PD1. Molecular dissection of this element reveals the presence of two overlapping functional activities: an enhancer and a silencer. Distinct nuclear factors bind to the overlapping enhancer and silencer DNA sequence elements within the regulatory domain. The levels of factors binding the silencer DNA sequence in different cell types are inversely related to levels of class I expression; in contrast, factors binding the enhancer DNA sequence can be detected in all cells. In cultured cell lines, inhibition of protein synthesis leads to the rapid loss of silencer complexes, with a concomitant increase in both enhancer complexes and MHC class I RNA. From these data, we conclude that a labile silencer factor competes with a constitutively expressed, stable enhancer factor for overlapping DNA-binding sites; the relative abundance of the silencer factor contributes to establishing steady-state levels of MHC class I gene expression. C1 NCI,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4B-17,BETHESDA,MD 20892. NR 30 TC 68 Z9 68 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD AUG PY 1991 VL 11 IS 8 BP 4217 EP 4227 PG 11 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FX723 UT WOS:A1991FX72300043 PM 2072915 ER PT J AU WEISSMAN, JD SINGER, DS AF WEISSMAN, JD SINGER, DS TI STRIKING SIMILARITIES BETWEEN THE REGULATORY MECHANISMS GOVERNING YEAST MATING-TYPE GENES AND MAMMALIAN MAJOR HISTOCOMPATIBILITY COMPLEX GENES SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID CLASS-I GENE; INTERFERON RESPONSE SEQUENCE; DNA-BINDING DOMAIN; REPRESSOR ALPHA-2; PROTEIN; TRANSCRIPTION; EXPRESSION; PROMOTER; OPERATOR; ENHANCER AB Expression of a mammalian major histocompatibility complex (MHC) class I gene is in part regulated by a silencer DNA sequence element which binds a complex of silencer factors. This negative regulatory system is shown to be strikingly similar to the yeast alpha-2 mating-type repression system. A moderate DNA sequence homology exists between the MHC class I silencer DNA element and the yeast alpha-2 operator. Mammalian silencer factors specifically bind to the yeast alpha-2 operator DNA and also specifically interact with a yeast alpha-2-binding protein. Furthermore, the alpha-2 operator functions as a silencer element in mammalian cells when placed upstream of a MHC class I promoter. C1 NCI,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4B-17,BETHESDA,MD 20892. NR 28 TC 20 Z9 20 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD AUG PY 1991 VL 11 IS 8 BP 4228 EP 4234 PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FX723 UT WOS:A1991FX72300044 PM 2072916 ER PT J AU VONDERHAAR, BK BANERJEE, R AF VONDERHAAR, BK BANERJEE, R TI IS TAMOXIFEN ALSO AN ANTI-LACTOGEN SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Article DE LACTOGEN; PROLACTIN; ANTIESTROGEN; TAMOXIFEN; ANTILACTOGEN BINDING SITE ID ANTIESTROGEN-BINDING-SITES; HUMAN-BREAST-CANCER; RABBIT MAMMARY-GLAND; PROLACTIN RECEPTOR; ESTROGEN-RECEPTOR; CELL-LINE; ENDOGENOUS LIGAND; GROWTH; RAT; INHIBITION RP VONDERHAAR, BK (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,BLD 10,RM 5B56,BETHESDA,MD 20892, USA. NR 56 TC 11 Z9 11 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD AUG PY 1991 VL 79 IS 1-3 BP C159 EP C163 DI 10.1016/0303-7207(91)90088-A PG 5 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA GA132 UT WOS:A1991GA13200006 PM 1936533 ER PT J AU CLARK, M POST, RM WEISS, SRB CAIN, CJ NAKAJIMA, T AF CLARK, M POST, RM WEISS, SRB CAIN, CJ NAKAJIMA, T TI REGIONAL EXPRESSION OF C-FOS MESSENGER-RNA IN RAT-BRAIN DURING THE EVOLUTION OF AMYGDALA KINDLED SEIZURES SO MOLECULAR BRAIN RESEARCH LA English DT Article DE C-FOS; MESSENGER RNA; INSITU HYBRIDIZATION; RAT BRAIN; AMYGDALA KINDLING; SEIZURE; AFTERDISCHARGE; RUNNING FIT ID LONG-TERM POTENTIATION; IMMEDIATE-EARLY GENES; MOUSE-BRAIN; GRANULE CELLS; DENTATE GYRUS; HIPPOCAMPUS; PROTEIN; STIMULATION; INDUCTION; PROENKEPHALIN AB The biochemical alterations eliciting the growth and spread of afterdischarge and accompanying the evolution of behavioral seizure stages in electrical kindling are not known. In situ hybridization for c-fos mRNA was used to map potential brain structures recruited during the evolution of major motor seizures from electrical kindling of the amygdala in rats. Two different patterns of c-fos induction were observed in the earliest stages of kindling (stages 1 and 2). A unilateral cortical distribution included the insular, temporal, perirhinal and parietal cortices and the amygdala. No changes in the hippocampus were noted in this group. The second distribution pattern was limited to the hippocampus (either unilateral or bilateral) and amygdala (unilateral) with no changes in the cortical areas. The afterdischarge durations were significantly (2 fold) longer in the 'hippocampal' group as compared to the 'cortical' group. In the later stages of kindling (stages 4 and 5) the distribution of c-fos mRNA was uniformly bilateral and involved a combination of the hippocampal and cortical distributions observed in the earlier stages and including the amygdala bilaterally as well. The induction of c-fos mRNA appears to provide a map of two different routes in the sequential pathways involved in the evolution of kindled seizures; it may also ultimately prove to be an important component of the kindling process itself. Additionally, c-fos mRNA was elevated bilaterally in the inferior colliculus of animals exhibiting running fits with their seizures. The inferior colliculus was previously shown by others to be involved in running fits accompanying convulsions. RP CLARK, M (reprint author), NIMH,BIOL PSYCHIAT BRANCH,9000 ROCKVILLE PIKE,BLDG 10,RM 3N212,BETHESDA,MD 20892, USA. NR 41 TC 106 Z9 106 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD AUG PY 1991 VL 11 IS 1 BP 55 EP 64 DI 10.1016/0169-328X(91)90021-O PG 10 WC Neurosciences SC Neurosciences & Neurology GA GP081 UT WOS:A1991GP08100008 ER PT J AU JOSHI, J SABOL, SL AF JOSHI, J SABOL, SL TI PROENKEPHALIN GENE-EXPRESSION IN C6 RAT GLIOMA-CELLS - POTENTIATION OF CYCLIC ADENOSINE 3',5'-MONOPHOSPHATE-DEPENDENT TRANSCRIPTION BY GLUCOCORTICOIDS SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID MESSENGER-RIBONUCLEIC-ACID; BOVINE CHROMAFFIN CELLS; MULTIHORMONAL REGULATION; CHLORAMPHENICOL ACETYLTRANSFERASE; ENKEPHALIN BIOSYNTHESIS; NEGATIVE REGULATION; RECEPTOR-BINDING; ADRENAL-MEDULLA; DNA ELEMENT; RNA AB Glucocorticoids enhance proenkephalin gene expression in several cell types. To elucidate the mechanism(s) involved, we analyzed the potentiation by dexamethasone of the cAMP-dependent increase in proenkephalin mRNA levels elicited by forskolin in C6 rat glioma cells. This potentiation did not require ongoing protein synthesis. In nuclear run-on transcription assays, dexamethasone alone did not alter proenkephalin transcription, but strongly increased the magnitude and duration of transcriptional elevation by forskolin through a direct action not requiring ongoing protein synthesis. Dexamethasone did not alter basal or stimulated cAMP levels. To search for functionally cooperative glucocorticoid and cAMP regulatory elements, we transfected C6 cells with plasmids containing the chloramphenicol acetyltransferase (CAT) gene under the control of rat proenkephalin sequences from bases -5800 to + 703. Maximum stimulation of transiently expressed CAT activity by forskolin required more than 145 and 190 or fewer base pairs of 5'-flanking sequence, implicating sequences up-stream from the previously described cAMP-inducible enhancer. Dexamethasone reduced forskolin-stimulated CAT expression from plasmids with 190 or more base-pairs of 5'-flanking sequence, an effect apparently involving multiple up-stream regions. Dexamethasone also reduced forskolin-stimulated CAT mRNA levels in C6 cells stably transfected with proenkephalin/CAT chimeric genes in the presence or absence of protein synthesis. In summary, we demonstrate that glucocorticoids and cAMP synergize positively in regulating transcription of the endogenous gene, but interact negatively in regulating the chimeric constructs, which may lack the context or distal element(s) required for positive synergism. C1 NHLBI,BIOCHEM GENET LAB,BLDG 36,ROOM 1C06,BETHESDA,MD 20892. NR 51 TC 63 Z9 63 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD AUG PY 1991 VL 5 IS 8 BP 1069 EP 1080 PG 12 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA GD003 UT WOS:A1991GD00300004 PM 1658636 ER PT J AU ORLOWSKI, CC OOI, GT BROWN, DR YANG, YWH TSENG, LYH RECHLER, MM AF ORLOWSKI, CC OOI, GT BROWN, DR YANG, YWH TSENG, LYH RECHLER, MM TI INSULIN RAPIDLY INHIBITS INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-1 GENE-EXPRESSION IN H4-II-E RAT HEPATOMA-CELLS SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID HUMAN-FETAL LIVER; FACTOR-I; MULTIHORMONAL REGULATION; DIABETES-MELLITUS; AMNIOTIC-FLUID; MESSENGER-RNA; FACTOR IGF; HORMONE; SERUM; IDENTIFICATION AB The insulin-like growth factor-binding proteins (IGFBPs) are thought to determine the distribution of IGF-I and IGF-II between the blood and tissue compartments and to modulate their biiological activities. A dynamic metabolic role for one of the IGFBPs, IGFBP-1, is suggested by the fact that plasma IGFBP-1 was increased after fasting and diabetes and rapidly decreased by refeeding or insulin treatment, respectively. IGFBP-1 mRNA also is increased in the livers of diabetic rats and decreased by insulin treatment. To understand the molecular basis for this regulation, we have examined the effects of insulin on IGFBP-1 and IGFBP-1 mRNA in the H4-II-E cell line derived from the well differentiated H35 rat hepatoma. IGFBP-1, identified by ligand blotting and immunoblotting, is the major IGFBP in H4-II-E cells. Incubation of H4-II-E cells with insulin for 24 h decreased IGFBP-1 in the culture medium by approximately 50%. Inhibition was observed at physiological concentrations of insulin (ED50, < 0.5 nM), but not at higher concentrations of IGF-II. These results, together with the fact that H4-II-E cells do not posses IGF-I receptors with which insulin might cross-react, suggest that insulin acts via the insulin receptor. Insulin inhibited IGFBP-1 in the medium by 80% in the absence of glucose, suggesting that the inhibition is a direct effect of insulin; glucose exerted a smaller independent effect in the absence of insulin. Insulin decreased IGFBP-1 mRNA in H4-II-E cells by 50% within 1 h and by 90% after 2-12 h of incubation. Nuclear run-on transcription assays indicated a corresponding decrease in the rate of IGFBP-1 gene transcription. Pretreatment of H4-II-E cells with dexamethasone stimulated IGFBP-1 transcription and increased steady state IGFBP-1 mRNA; stimulation was abolished by insulin treatment, indicating that inhibition by insulin was dominant over induction by dexamethasone. Thus, insulin, acting through the insulin receptor, rapidly decreases the abundance of IGFBP-1 mRNA in H4-II-E cells. Regulation occurs at least in part at the level of gene transcription. We propose that regulation of IGFBP-1 synthesis is an important component of the regulation of IGFBP-1 by insulin in vivo. C1 NIDDKD,MOLEC CELLULAR & NUTR ENDOCRINOL BRANCH,GROWTH & DEV SECT,BLDG 10,ROOM 8D-14,BETHESDA,MD 20892. NR 46 TC 92 Z9 92 U1 0 U2 1 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD AUG PY 1991 VL 5 IS 8 BP 1180 EP 1187 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA GD003 UT WOS:A1991GD00300016 PM 1719386 ER PT J AU HARINDRANATH, N ALEXANDER, CB MAGE, RG AF HARINDRANATH, N ALEXANDER, CB MAGE, RG TI EVOLUTIONARILY CONSERVED ORGANIZATION AND SEQUENCES OF GERMLINE DIVERSITY AND JOINING REGIONS OF THE RABBIT T-CELL RECEPTOR-BETA-2 CHAIN SO MOLECULAR IMMUNOLOGY LA English DT Article ID BETA-CHAIN; CONSTANT-REGION; ANTIGEN RECEPTOR; VARIABLE-REGION; GENE SEGMENTS; IDENTIFICATION; ALLOTYPES; ELEMENT; VECTORS; CLONING AB We previously reported that there are three different copies of T-cell receptor beta-chain constant region (C-beta) genes in some rabbits, two of which are present on an approximately 16-kb and one on an approximately 6-kb Eco RI fragment. We also reported that one of the C-beta genes on the approximately 16-kb fragment was chimeric, with a 5' cluster of J-beta-2 segments and a 3' untranslated region of beta-1 type. Here we report the complete genomic sequences of the D-beta-2 and J-beta-2 segments associated with the chimeric C-beta gene. The rabbit D-beta-2 gene segment has very strong similarity to both its human and mouse counterparts. The sequence similarity also extends rather far from the coding region in both 5' and 3' directions. The content and organization of rabbit J-beta-2 gene segments is similar to those found in both human and mouse. The rabbit J-beta-2 cluster has six functional segments and one pseudogene, as well as a remnant of another pseudogene between J-beta-2.2 and J-beta-2.3 equivalent to the one found in man in the same location. The J-beta-2.5 gene segment of rabbit has lost the splice signal and is a pseudogene unlike its counterparts in man and mouse. Overall analysis of the rabbit D-beta-2-J-beta-2 region reveals a closer similarity to human than mouse. However, the general organization of the gene segments in the D-beta-2-J-beta-2 regions of all three species is remarkably conserved over long stretches of DNA sequence. C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. NR 25 TC 6 Z9 6 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0161-5890 J9 MOL IMMUNOL JI Mol. Immunol. PD AUG PY 1991 VL 28 IS 8 BP 881 EP 888 DI 10.1016/0161-5890(91)90052-L PG 8 WC Biochemistry & Molecular Biology; Immunology SC Biochemistry & Molecular Biology; Immunology GA GA788 UT WOS:A1991GA78800010 PM 1678859 ER PT J AU CURCIO, MJ GARFINKEL, DJ AF CURCIO, MJ GARFINKEL, DJ TI REGULATION OF RETROTRANSPOSITION IN SACCHAROMYCES-CEREVISIAE SO MOLECULAR MICROBIOLOGY LA English DT Review ID VIRUS-LIKE PARTICLES; TY1 ELEMENT TRANSPOSITION; TRANSFER-RNA GENES; REVERSE-TRANSCRIPTASE; YEAST; INTERMEDIATE; SEQUENCES; CIS AB Retrotransposons are a widely distributed group of eukaryotic mobile genetic elements that transpose through an RNA intermediate. The element Ty (Transposon yeast), found in the yeast Saccharomyces cerevisiae, is a model system for the study of retrotransposons because of the experimental tools that exist to manipulate and detect transposition. Ty transposition can be elevated to levels exceeding one transposition event per cell when an element is expressed from an inducible yeast promoter. In addition, individual genomic Ty elements can be tagged with a retrotransposition indicator gene that allows transposition events occurring at at a rate of 10(-5) to 10(-7) per element per cell division to be detected phenotypically. These systems are being used to elucidate the mechanism of Ty transposition and clarify how Ty transposition is controlled. C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,POB B,BLDG 539,FREDERICK,MD 21702. OI Curcio, M. Joan/0000-0001-5361-3909 FU NCI NIH HHS [N01-CO-74101] NR 27 TC 24 Z9 24 U1 1 U2 1 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0950-382X J9 MOL MICROBIOL JI Mol. Microbiol. PD AUG PY 1991 VL 5 IS 8 BP 1823 EP 1829 DI 10.1111/j.1365-2958.1991.tb00806.x PG 7 WC Biochemistry & Molecular Biology; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA GC394 UT WOS:A1991GC39400002 PM 1662752 ER EF