FN Thomson Reuters Web of Science™ VR 1.0 PT J AU BUNNETT, N HWANG, L MENOZZI, D GU, Z MATON, P AF BUNNETT, N HWANG, L MENOZZI, D GU, Z MATON, P TI NEUTRAL ENDOPEPTIDASE (NEP) DOWN REGULATES THE CONTRACTILE EFFECTS OF GASTRIN RELEASING PEPTIDE (GRP10) ON GASTRIC MUSCLE-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. NIDDK,DIGEST DIS BRANCH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A387 EP A387 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700096 ER PT J AU CARRENO, BM COLIGAN, J BIDDISON, WE AF CARRENO, BM COLIGAN, J BIDDISON, WE TI HLA-B27 BINDS A RESTRICTED REPERTOIRE OF PEPTIDES AS ASSESSED IN COMPETITIVE-INHIBITION ASSAYS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINCDS,NIB,BETHESDA,MD 20892. NIAID,BIOL RESOURCES BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A724 EP A724 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702053 ER PT J AU CASTRONOVO, V CIOCE, V BARKER, KT SOBEL, ME AF CASTRONOVO, V CIOCE, V BARKER, KT SOBEL, ME TI GALAMS - A FAMILY OF CELL-SURFACE GALACTOSIDE BINDING LECTINS THAT BIND SPECIFICALLY TO LAMININ SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,PATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A447 EP A447 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700440 ER PT J AU CAVIEDES, P GRANT, D VARGAS, FF AF CAVIEDES, P GRANT, D VARGAS, FF TI ALBUMIN-INDUCED CHANGES IN ELECTRICAL MEMBRANE-PROPERTIES OF CULTURED ENDOTHELIAL-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,CELL BIOL & GENET LAB,BETHESDA,MD 20892. NIDR,DEV BIOL & ANOMALIES LAB,BETHESDA,MD 20892. US FDA,CDER,ODEI,DIV CARIORENAL DRUG,ROCKVILLE,MD 20857. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A698 EP A698 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701904 ER PT J AU CHEN, LS COLEMAN, WG AF CHEN, LS COLEMAN, WG TI CLONING AND CHARACTERIZATION OF A-RFA LOCUS THAT IS REQUIRED FOR HEPTOSE ADDITION TO LIPOPOLYSACCHARID IN ESCHERICHIA-COLI K-12 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A837 EP A837 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702711 ER PT J AU CITRON, BA LEVINE, RA MILSTIEN, S KAUFMAN, S AF CITRON, BA LEVINE, RA MILSTIEN, S KAUFMAN, S TI RAT SEPIAPTERIN REDUCTASE PROTEIN AND MESSENGER-RNA SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,NEUROCHEM LAB,BETHESDA,MD 20892. LAFAYETTE CLIN,DETROIT,MI 48207. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A458 EP A458 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700504 ER PT J AU CLERICI, M BERZOFSKY, A SHEARER, GM AF CLERICI, M BERZOFSKY, A SHEARER, GM TI CYTOTOXIC LYMPHOCYTE-T SPECIFIC FOR GP160 PEPTIDES IN HIV SEROPOSITIVE INDIVIDUALS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20852. NIH,METAB BRANCH,BETHESDA,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A598 EP A598 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701321 ER PT J AU COMMENT, C POLLOCK, P BLAYLOCK, B GERMOLEC, D KOUCHI, Y LUSTER, M AF COMMENT, C POLLOCK, P BLAYLOCK, B GERMOLEC, D KOUCHI, Y LUSTER, M TI CHEMICAL INDUCED THYMIC ATROPHY EXAMINED FOR DIRECT MECHANISMS ON THYMOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A551 EP A551 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701045 ER PT J AU COUSINS, SW MCCABE, M DANIELPOUR, D STREILEIN, JW AF COUSINS, SW MCCABE, M DANIELPOUR, D STREILEIN, JW TI TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) IMMUNOSUPPRESSIVE MOLECULE IN AQUEOUS-HUMOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MIAMI,SCH MED,MIAMI,FL 33136. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A628 EP A628 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701495 ER PT J AU DAVIS, MD KAUFMAN, S MILSTIEN, S AF DAVIS, MD KAUFMAN, S MILSTIEN, S TI INTERCONVERSION OF 6-SUBSTITUTED AND 7-SUBSTITUTED TETRAHYDROPTERINS VIA PHENYLALANINE-HYDROXYLASE GENERATED INTERMEDIATES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,NEUROCHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A418 EP A418 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700272 ER PT J AU DING, L SETO, BL COLEMAN, WG AF DING, L SETO, BL COLEMAN, WG TI PURIFICATION OF ADP-L-GLYCERO-D-MANNOHEPTOSE-6-EPIMERASE IN ESCHERICHIA-COLI K-12 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDKD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A788 EP A788 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702425 ER PT J AU DORGAN, J ZIEGLER, R SCHOENBERG, J SHAW, G MCADAMS, M AF DORGAN, J ZIEGLER, R SCHOENBERG, J SHAW, G MCADAMS, M TI RACE AND SEX-DIFFERENCES IN THE ASSOCIATION OF FRUIT AND VEGETABLE CONSUMPTION WITH LUNG-CANCER RISK SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NEW JERSEY STATE DEPT HLTH,TRENTON,NJ 08625. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A562 EP A562 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701109 ER PT J AU DORTCH, J MALEQUE, MA KOPSOMBUT, P BROSSI, A AF DORTCH, J MALEQUE, MA KOPSOMBUT, P BROSSI, A TI EFFECTS OF WEBERINE AND NORWEBERINE ON RECEPTOR MEDIATED RESPONSES IN AORTIC SMOOTH-MUSCLE SO FASEB JOURNAL LA English DT Meeting Abstract C1 MEHARRY MED COLL,DEPT PHARMACOL,NASHVILLE,TN 37208. NIH,MED CHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A492 EP A492 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700703 ER PT J AU DRISCOLL, WJ LEE, YC STROTT, CA AF DRISCOLL, WJ LEE, YC STROTT, CA TI ADRENOCORTICAL PREGNENOLONE-BINDING PROTEIN-ACTIVITY REQUIRES A SMALL, PHOSPHORYLATED HEAT-STABLE FACTOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,ENDOCRINOL & REPROD RES BRANCH,ADRENAL CELL BIOL SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A797 EP A797 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702480 ER PT J AU FEDARKO, NS VETTER, UK ROBEY, PG AF FEDARKO, NS VETTER, UK ROBEY, PG TI DEVELOPMENTAL REGULATION OF PROTEOGLYCANS, HYALURONAN AND COLLAGEN-METABOLISM BY HUMAN BONE-CELLS IN CULTURE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. RI Robey, Pamela/H-1429-2011 OI Robey, Pamela/0000-0002-5316-5576 NR 0 TC 3 Z9 3 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A444 EP A444 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700423 ER PT J AU FEUILLAN, P AGUILERA, G AF FEUILLAN, P AGUILERA, G TI ANGIOTENSIN-II RECEPTOR SUBTYPES IN THE RAT FETUS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,DEB,BETHESDA,MD 20892. NR 0 TC 7 Z9 7 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A872 EP A872 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702913 ER PT J AU FISHER, A TANIUCHI, H AF FISHER, A TANIUCHI, H TI THE CORE INTERACTION LOOPS THAT DRIVE FOLDING OF CYTOCHROME-C SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A803 EP A803 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702513 ER PT J AU FISHER, MT AF FISHER, MT TI CHAPERONIN-60 (GROEL) INTERACTIONS WITH ESCHERICHIA-COLI GLUTAMINE-SYNTHETASE DURING RENATURATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A433 EP A433 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700359 ER PT J AU FLESSNER, M DEDRICK, R AF FLESSNER, M DEDRICK, R TI CONCENTRATION PROFILES IN NORMAL TISSUE AND TUMOR AFTER IV OR IP INJECTION OF MONOCLONAL-ANTIBODY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A755 EP A755 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702230 ER PT J AU FORSBERG, G ZACHRISSON, K LARSSON, B LEVINE, RL HARTMANIS, M AF FORSBERG, G ZACHRISSON, K LARSSON, B LEVINE, RL HARTMANIS, M TI AUTOPROTEOLYTIC CLEAVAGE OF PURIFIED HIV-1 PROTEASE SO FASEB JOURNAL LA English DT Meeting Abstract C1 KABIGEN AB,STOCKHOLM,SWEDEN. NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. RI Levine, Rodney/D-9885-2011 NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A453 EP A453 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700474 ER PT J AU FUJIMOTO, Y HAMPTON, LL SILVERMAN, JA LUO, L THORGEIRSSON, SS WIRTH, PJ AF FUJIMOTO, Y HAMPTON, LL SILVERMAN, JA LUO, L THORGEIRSSON, SS WIRTH, PJ TI MOLECULAR CHARACTERIZATION OF RAT-LIVER EPITHELIAL (RLE) CELL-LINES TRANSFORMED BY AFLATOXIN-B1 (AFB) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A881 EP A881 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702968 ER PT J AU FUKAMAUCHI, F HOUGH, C CHUANG, DM AF FUKAMAUCHI, F HOUGH, C CHUANG, DM TI AGONIST-INDUCED AND ANTAGONIST-INDUCED MODULATION OF M2-MUSCARINIC AND M3-MUSCARINIC CHOLINERGIC RECEPTOR MESSENGER-RNA IN CULTURED CEREBELLAR NEURONS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,BIOL PSYCHIAT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A512 EP A512 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700817 ER PT J AU GAMBASSI, G CAPOGROSSI, MC SPURGEON, HA LAKATTA, EG AF GAMBASSI, G CAPOGROSSI, MC SPURGEON, HA LAKATTA, EG TI DISSECTION OF DIRECT MYOFILAMENT EFFECTS VERSUS PHOSPHO-DIESTERASE INHIBITION BY ENANTIOMERS OF A NOVEL CARDIOTONIC AGENT EMD-53998 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BETHESDA,MD 20892. NR 1 TC 2 Z9 2 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A493 EP A493 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700707 ER PT J AU GANT, TW SILVERMAN, JA MARINO, PA THORGEIRSSON, SS AF GANT, TW SILVERMAN, JA MARINO, PA THORGEIRSSON, SS TI RECEPTOR AND TRANS-ACTING FACTOR MEDIATED TRANSCRIPTIONAL CONTROL OF MULTIDRUG RESISTANCE GENE-EXPRESSION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A824 EP A824 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702634 ER PT J AU GARCIA, GE STADTMAN, TC AF GARCIA, GE STADTMAN, TC TI TGA CODES FOR SELENOCYSTEINE IN THE SELENOPROTEIN A COMPONENT OF THE GLYCINE REDUCTASE COMPLEXES OF CLOSTRIDIUM-PURINOLYTICUM AND CLOSTRIDIUM-STICKLANDII SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A814 EP A814 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702575 ER PT J AU GORDON, AW COLEMAN, WG AF GORDON, AW COLEMAN, WG TI CLONING AND SEQUENCE-ANALYSIS OF THE RFA--32 GENE IN THE 81-MINUTE REGION OF THE GENETIC-MAP OF ESCHERICHIA-COLI K-12 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDKD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A837 EP A837 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702709 ER PT J AU GORDON, MR SAWASDIKOSOL, S HAGUE, BF BOWERS, F KINDT, TJ AF GORDON, MR SAWASDIKOSOL, S HAGUE, BF BOWERS, F KINDT, TJ TI HTLV-I INFECTS AND TRANSFORMS CELLS OTHER THAN T-CELLS IN THE RABBIT SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOGENET LAB,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A597 EP A597 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701315 ER PT J AU HAGUE, BF RECKER, DP LEE, K KINDT, TJ AF HAGUE, BF RECKER, DP LEE, K KINDT, TJ TI ROLE OF CD4 IN HIV-1 INFECTION OF RABBIT-CELL LINES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOGENET LAB,TWINBROOK FACIL,ROCKVILLE,MD 20852. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A649 EP A649 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701618 ER PT J AU HELM, BA PADLAN, EA AF HELM, BA PADLAN, EA TI A NEW MODEL STRUCTURE FOR HUMAN IGE-FC SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV SHEFFIELD,KREBS INST BIOMOLEC RES,SHEFFIELD S10 2UH,ENGLAND. NIDDK,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A734 EP A734 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702112 ER PT J AU HIRAMATSU, Y HORN, VJ BAUM, BJ AMBUDKAR, IS AF HIRAMATSU, Y HORN, VJ BAUM, BJ AMBUDKAR, IS TI POLYPHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C IN RAT PAROTID MEMBRANES - MUSCARINIC RECEPTOR AND G-PROTEIN REGULATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,CIPCB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A481 EP A481 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700636 ER PT J AU HONG, HL BOORMAN, GA AF HONG, HL BOORMAN, GA TI RESIDUAL MARROW EFFECT OF LINDANE IN MICE FOLLOWING IRRADIATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A485 EP A485 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700662 ER PT J AU HORAN, PJ DECOSTA, BR RICE, KC PORRECA, F AF HORAN, PJ DECOSTA, BR RICE, KC PORRECA, F TI DIFFERENTIAL ANTAGONISM OF U69,593-MEDIATED AND BREMAZOCINE-MEDIATED ANTINOCICEPTION BY UPHIT SUPPORTS KAPPA-OPIOID RECEPTOR MULTIPLICITY SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV ARIZONA,ARIZONA HLTH SCI CTR,DEPT PHARMACOL,TUCSON,AZ 85724. NIDDK,MED CHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A474 EP A474 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700596 ER PT J AU ISHAC, EJN LAZARWESLEY, E SZENTENDREI, T HADCOCK, JR MALBON, CC KUNOS, G AF ISHAC, EJN LAZARWESLEY, E SZENTENDREI, T HADCOCK, JR MALBON, CC KUNOS, G TI TIME-DEPENDENT CHANGES IN ALPHA-1-ADRENOCEPTOR AND BETA-2-ADRENOCEPTOR (ALPHA-1AR AND BETA-2AR) MESSENGER-RNA IN ISOLATED RAT-LIVER CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,LPPS,ROCKVILLE,MD 20852. SUNY STONY BROOK,DEPT PHARMACOL,STONY BROOK,NY 11794. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A513 EP A513 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700826 ER PT J AU JICHA, DL MULE, JJ CUSTER, M TAUBENBERGER, J ROSENBERG, SA AF JICHA, DL MULE, JJ CUSTER, M TAUBENBERGER, J ROSENBERG, SA TI SURVIVAL OF HUMAN PERIPHERAL LYMPHOCYTES (PBMC) AND TUMOR INFILTRATING LYMPHOCYTES (TIL) IN IMMUNODEFICIENT MICE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,SURG BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A637 EP A637 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701549 ER PT J AU JOHNSON, C AGUILERA, G AF JOHNSON, C AGUILERA, G TI CHANGES IN ANGIOTENSIN-II RECEPTOR SUBTYPES AND COUPLING TO ADENYLATE-CYCLASE IN CULTURED FETAL FIBROBLASTS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,DEB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A872 EP A872 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702914 ER PT J AU JONES, CR NIMS, RW LUBET, RA AF JONES, CR NIMS, RW LUBET, RA TI INDUCTION OF ALDEHYDE DEHYDROGENASE BY PHENOBARBITAL-TYPE COMPOUNDS IN COPENHAGEN RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 PRI DYNCORP,FREDERICK,MD. NCI,FREDERIE CANC RES & DEV CTR,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21701. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A825 EP A825 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702637 ER PT J AU KANG, CY NARA, P CHAMAT, S CARALLI, V RYSKAMP, T HAIGWOOD, N NEWMAN, R KOHLER, H AF KANG, CY NARA, P CHAMAT, S CARALLI, V RYSKAMP, T HAIGWOOD, N NEWMAN, R KOHLER, H TI EVIDENCE FOR NON-V3 SPECIFIC NEUTRALIZING ANTIBODIES IN HIV-1 INFECTED HUMANS WHICH INTERFERE WITH GP120/CD4 BINDING SO FASEB JOURNAL LA English DT Meeting Abstract C1 IDEC PHARMACEUT CORP,LA JOLLA,CA 92037. NCI,FREDERICK,MD 21701. CHIRON CORP,EMERYVILLE,CA 94608. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A598 EP A598 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701317 ER PT J AU KANT, AK SCHATZKIN, A BLOCK, G HARRIS, T ZIEGLER, R AF KANT, AK SCHATZKIN, A BLOCK, G HARRIS, T ZIEGLER, R TI DIETARY DIVERSITY AND MORTALITY IN THE NHANES-I EPIDEMIOLOGIC FOLLOW-UP-STUDY SO FASEB JOURNAL LA English DT Meeting Abstract C1 CUNY QUEENS COLL,FLUSHING,NY 11367. NCI,BETHESDA,MD 20892. RI Block, Gladys/E-3304-2010 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A715 EP A715 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701999 ER PT J AU KARLSTROM, AR LEVINE, RL AF KARLSTROM, AR LEVINE, RL TI COPPER INHIBITS THE HIV-1 PROTEASE BY BOTH OXYGEN-DEPENDENT AND OXYGEN-INDEPENDENT MECHANISMS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. RI Levine, Rodney/D-9885-2011 NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A452 EP A452 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700473 ER PT J AU KARP, DR TELETSKI, CL LONG, EO AF KARP, DR TELETSKI, CL LONG, EO TI IDENTIFICATION OF HLA-DR BETA-CHAIN RESIDUES RESPONSIBLE FOR BINDING OF STAPHYLOCOCCAL TOXINS SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV TEXAS,SW MED CTR,DALLAS,TX 75235. NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A727 EP A727 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702073 ER PT J AU KATZ, A CASTILLO, C SPENCER, MK YAN, Z NYOMBA, BL AF KATZ, A CASTILLO, C SPENCER, MK YAN, Z NYOMBA, BL TI EFFECT OF FASTING ON INSULIN-MEDIATED CARBOHYDRATE-METABOLISM IN HUMAN MUSCLE SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV ILLINOIS,URBANA,IL 61801. NIDDK,PHOENIX,AZ 85016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A653 EP A653 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701641 ER PT J AU KIM, HK KIM, JW RHEE, SG AF KIM, HK KIM, JW RHEE, SG TI PDGF STIMULATION OF INOSITOL PHOSPHOLIPID HYDROLYSIS REQUIRES PLC-GAMMA-PHOSPHORYLATION AT TYROSINE-783 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A482 EP A482 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700644 ER PT J AU KIM, JW MIN, HK RHEE, SG AF KIM, JW MIN, HK RHEE, SG TI IDENTIFICATION OF A GROWTH FACTOR-DEPENDENT SERINE PHOSPHORYLATION SITE OF PHOSPHOLIPASE-C-GAMMA INVITRO AND INVIVO SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A482 EP A482 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700647 ER PT J AU KIRKNESS, EF KUSIAK, JW FLEMING, JT MENNINGER, J GOCAYNE, JD WARD, DC VENTER, JC FRASER, CM AF KIRKNESS, EF KUSIAK, JW FLEMING, JT MENNINGER, J GOCAYNE, JD WARD, DC VENTER, JC FRASER, CM TI CHARACTERIZATION OF THE GENE ENCODING THE HUMAN GABA-A RECEPTOR BETA-1 SUBUNIT SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,MOLEC NEUROBIOL SECT,ROCKVILLE,MD 20852. NINCDS,LMCN,BETHESDA,MD 20892. YALE UNIV,SCH MED,DEPT HUMAN GENET,NEW HAVEN,CT 06510. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A854 EP A854 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702813 ER PT J AU KONG, SK CHOCK, PB AF KONG, SK CHOCK, PB TI REGULATION OF PROTEIN UBIQUITINATION - A POTENTIAL ROLE FOR PROTEIN PHOSPHORYLATION/DEPHOSPHORYLATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A428 EP A428 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700330 ER PT J AU KOZLOW, EJ KEHRL, JH FAUCI, AS RIECKMANN, P AF KOZLOW, EJ KEHRL, JH FAUCI, AS RIECKMANN, P TI THE ROLE OF AUTOCRINE IL-6 AND TNF-ALPHA PRODUCTION IN HUMAN B-CELL FUNCTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A603 EP A603 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701346 ER PT J AU KOZLOWSKI, S TAKESHITA, T BOEHNCKE, WH TAKAHASHI, H BOYD, LF GERMAIN, RN BERZOFSKY, JA MARGULIES, DH AF KOZLOWSKI, S TAKESHITA, T BOEHNCKE, WH TAKAHASHI, H BOYD, LF GERMAIN, RN BERZOFSKY, JA MARGULIES, DH TI FREE BETA-2-MICROGLOBULIN ENHANCES FUNCTIONAL ASSOCIATION OF PEPTIDE WITH PURIFIED CLASS-I MHC MOLECULES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NCI,BETHESDA,MD 20892. RI Margulies, David/H-7089-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A724 EP A724 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702055 ER PT J AU KRUEGER, GRF SANDER, C TESCH, H JUECKER, M ABLASHI, DV BALACHANDRAN, N DIEHL, V GALLO, RC AF KRUEGER, GRF SANDER, C TESCH, H JUECKER, M ABLASHI, DV BALACHANDRAN, N DIEHL, V GALLO, RC TI INFECTION OF HODGKIN CELL-CULTURES BY HUMAN HERPES-VIRUS-6 (HHV-6) SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV COLOGNE,SCH MED,W-5000 COLOGNE 41,GERMANY. NCI,BETHESDA,MD 20892. UNIV KANSAS,SCH MED,LAWRENCE,KS 66045. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A706 EP A706 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701951 ER PT J AU LAKATTA, EG OCONNOR, F SCHULMAN, S GERSTENBLITH, G BECKER, L FLEG, JL AF LAKATTA, EG OCONNOR, F SCHULMAN, S GERSTENBLITH, G BECKER, L FLEG, JL TI CARDIAC VOLUMES AT REST AND DURING CYCLE EXERCISE IN HEALTHY-MEN OF A BROAD AGE RANGE - EFFECT OF FITNESS MATCHING SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 1 Z9 1 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A766 EP A766 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702299 ER PT J AU LAWTON, MP KRONBACH, T JOHNSON, EF PHILPOT, RM AF LAWTON, MP KRONBACH, T JOHNSON, EF PHILPOT, RM TI PROPERTIES OF EXPRESSED AND NATIVE FLAVIN-CONTAINING MONOOXYGENASES - EVIDENCE FOR MULTIPLE FORMS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. SCRIPPS CLIN & RES FDN,LA JOLLA,CA 92037. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A843 EP A843 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702748 ER PT J AU LEBON, TR KASUYA, J BELFRAGE, P MANGANIELLO, VC FUJITAYAMAGUCHI, Y AF LEBON, TR KASUYA, J BELFRAGE, P MANGANIELLO, VC FUJITAYAMAGUCHI, Y TI PURIFICATION AND CHARACTERIZATION OF CGMP-INHIBITED LOW KM CAMP PHOSPHODIESTERASE (PDE) FROM HUMAN PLACENTAL CYTOSOLIC FRACTIONS SO FASEB JOURNAL LA English DT Meeting Abstract C1 CITY HOPE NATL MED CTR,BECKMAN RES INT,DUARTE,CA 91010. NIH,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A454 EP A454 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700485 ER PT J AU LEE, NH FRASER, CM AF LEE, NH FRASER, CM TI REGULATION AND CROSS-TALK OF THE CLONED M1 MUSCARINIC AND BETA-2 ADRENERGIC-RECEPTORS COEXPRESSED IN CHO CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,ADAMHA,LPPS,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A512 EP A512 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700820 ER PT J AU LI, R WING, LL KIRCH, DG WYATT, RJ CHUANG, DM AF LI, R WING, LL KIRCH, DG WYATT, RJ CHUANG, DM TI EFFECTS OF CHRONIC NICOTINE AND HALOPERIDOL ADMINISTRATION ON MUSCARINIC RECEPTOR-MEDIATED PHOSPHOINOSITIDE TURNOVER IN RAT-BRAIN SLICES SO FASEB JOURNAL LA English DT Meeting Abstract C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. NIMH,BIOL PSYCHIAT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A480 EP A480 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700635 ER PT J AU LIAO, SY LUKA, J GOLDSCHMIDTS, W PURTILO, D AF LIAO, SY LUKA, J GOLDSCHMIDTS, W PURTILO, D TI EXPRESSION OF EPSTEIN-BARR-VIRUS (EBV) GENOME AND ANTIBODY-TITERS IN PATIENTS WITH GYNECOLOGICAL CARCINOMAS AS AN INDEX OF IMMUNODEFICIENCY SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CALIF IRVINE,IRVINE,CA 92668. NCI,PEDIAT ONCOL BRANCH,BETHESDA,MD 20814. UNIV NEBRASKA MED CTR,OMAHA,NE 68198. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A705 EP A705 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701946 ER PT J AU LONGNECKER, MP TAYLOR, PR LEVANDER, OA FLACK, V VEILLON, C MCADAM, PA PATTERSON, KY HOLDEN, J STAMPFER, MJ MORRIS, JS WILLETT, WC AF LONGNECKER, MP TAYLOR, PR LEVANDER, OA FLACK, V VEILLON, C MCADAM, PA PATTERSON, KY HOLDEN, J STAMPFER, MJ MORRIS, JS WILLETT, WC TI ESTIMATION OF SELENIUM (SE) INTAKE FROM SE IN SERUM, WHOLE-BLOOD, TOENAILS, OR URINE SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,SPH,LOS ANGELES,CA 90024. NCI,CPSB,DCPC,ROCKVILLE,MD. NCI,BETHESDA,MD 20892. HARVARD UNIV,SPH,BOSTON,MA 02115. UNIV MISSOURI,COLUMBIA,MO 65201. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A716 EP A716 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702007 ER PT J AU LUCE, GEG HORGAN, KJ TANAKA, Y SHIMIZU, Y SHAW, S AF LUCE, GEG HORGAN, KJ TANAKA, Y SHIMIZU, Y SHAW, S TI REGULATION OF ADHESION MOLECULES WITH T-CELL DIFFERENTIATION - 3 COLOR ANALYSIS OF HUMAN CD4+ T-CELL SUBSETS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,E1B,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A620 EP A620 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701446 ER PT J AU LUKA, J GOLDSCHMIDTS, W PURTILO, DT AF LUKA, J GOLDSCHMIDTS, W PURTILO, DT TI ISOLATION OF A NEW AGENT FROM AN EBV-GENOME NEGATIVE BURKITTS-LYMPHOMA CELL-LINE WHICH IMMORTALIZES HUMAN PERIPHERAL LYMPHOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV NEBRASKA MED CTR MED CTR,DEPT PATHOL & MICROBIOL,OMAHA,NE 68198. NCI,PEDIAT ONCOL BRANCH,BETHESDA,MD 20814. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A706 EP A706 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701948 ER PT J AU MACKIE, L JANG, S UYESAKA, N RODGERS, G AF MACKIE, L JANG, S UYESAKA, N RODGERS, G TI THE INFLUENCE OF HYDROXYUREA TREATMENT ON THE RHEOLOGICAL PROPERTIES OF SICKLE ERYTHROCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 CATHOLIC UNIV AMER,WASHINGTON,DC 20064. NIPPON MED COLL,TOKYO 113,JAPAN. NIH,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A685 EP A685 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701828 ER PT J AU MAKI, A YAMAMOTO, N SWANN, JD BEREZESKY, IK HOLBROOK, NJ TRUMP, BF AF MAKI, A YAMAMOTO, N SWANN, JD BEREZESKY, IK HOLBROOK, NJ TRUMP, BF TI OXIDANT INJURY INDUCES ACUTE AND STRESS GENES IN RAT PROXIMAL TUBULE EPITHELIUM (PTE) - THE ROLE OF CYTOSOLIC IONIZED CALCIUM ([CA2+]I) SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MARYLAND,SCH MED,DEPT PATHOL,BALTIMORE,MD 21201. MARYLAND INST EMERGENCY MED SERV SYST,BALTIMORE,MD 21201. NIA,GERONTOL RES CTR,MOLEC GENET LAB,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A705 EP A705 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701945 ER PT J AU MEACCI, E MOOS, M TAIRA, M SMITH, CJ VASTA, V DEGERMAN, E MOVSESIAN, MA BELFRAGE, P MANGANIELLO, VC AF MEACCI, E MOOS, M TAIRA, M SMITH, CJ VASTA, V DEGERMAN, E MOVSESIAN, MA BELFRAGE, P MANGANIELLO, VC TI CLONING OF CDNA FOR A HUMAN CARDIAC CGMP-INHIBITED LOW KM CAMP PHOSPHODIESTERASE (CGI PDE) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,CELL METAB LAB,BETHESDA,MD 20892. UNIV LUND,S-22100 LUND,SWEDEN. UNIV UTAH,MED CTR,SALT LAKE CITY,UT 84112. UNIV FLORENCE,I-50121 FLORENCE,ITALY. RI Moos, Malcolm/F-3673-2011 OI Moos, Malcolm/0000-0002-9575-9938 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A454 EP A454 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700482 ER PT J AU MINATO, S ASOFSKY, R AF MINATO, S ASOFSKY, R TI A 2-SITE ELISA ASSAY FOR MURINE INTERLEUKIN-4 (IL-4) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A617 EP A617 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701428 ER PT J AU MITTENBUHLER, K LEVINE, RL AF MITTENBUHLER, K LEVINE, RL TI HIV PROTEASE - CONSTRUCTION OF A RECOMBINANT BACULOVIRUS FOR PRODUCTION AND AFFINITY PURIFICATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. RI Levine, Rodney/D-9885-2011 NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A453 EP A453 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700477 ER PT J AU MORLEY, D VOSACEK, R KEEFER, L BOVE, AA MARAGOS, C WINK, D DUNAMS, TM HRABIE, JA AF MORLEY, D VOSACEK, R KEEFER, L BOVE, AA MARAGOS, C WINK, D DUNAMS, TM HRABIE, JA TI VASORELAXANT POTENCY OF DIETHYLAMINE NITRIC-OXIDE ADDUCT IS SIMILAR TO TRADITIONAL NITRATES SO FASEB JOURNAL LA English DT Meeting Abstract C1 TEMPLE UNIV,HLTH SCI CTR,SCH MED,PHILADELPHIA,PA 19140. NCI,FREDERICK CANC RES FACIL,FREDERICK,MD 21701. PRI DYN CORP,FREDERICK,MD 21702. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A385 EP A385 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700088 ER PT J AU MURAYAMA, M AF MURAYAMA, M TI PLATELET-RICH PLASMA-VOLUME INCREASES DURING DECOMPRESSION-INDUCED SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAMS,PHYS BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A902 EP A902 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20703092 ER PT J AU NETA, R KELLER, JR OPPENHEIM, JJ RUSCETTI, FW DUBOIS, CM AF NETA, R KELLER, JR OPPENHEIM, JJ RUSCETTI, FW DUBOIS, CM TI GLUCOCORTICOSTEROIDS (GC) AND CSF MEDIATE IL-1 INDUCED INVIVO UP-REGULATION OF IL-1 RECEPTOR ON BONE-MARROW CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 ARMED FORCES RADIOBIOL RES INST,BETHESDA,MD 20814. NCI,BRMP,FREDERICK,MD 21701. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A624 EP A624 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701471 ER PT J AU NIKBAKHT, KN LAWTON, MP PHILPOT, RM AF NIKBAKHT, KN LAWTON, MP PHILPOT, RM TI EVIDENCE THAT MULTIPLE PULMONARY FLAVIN-CONTAINING MONOOXYGENASES IN GUINEA-PIG AND RABBIT ARE ALLELIC VARIANTS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A843 EP A843 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702747 ER PT J AU OVERBY, LH GARDLIK, S PHILPOT, RM SERABJITSINGH, CJ AF OVERBY, LH GARDLIK, S PHILPOT, RM SERABJITSINGH, CJ TI DISCRETE CELLULAR-LOCALIZATION OF RABBIT ALPHA-CLASS GLUTA-THIONE S-TRANSFERASES (GST) IN KIDNEY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. GLAXO INC,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A502 EP A502 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700762 ER PT J AU PADMANABHAN, R GIORDANO, T TAN, T THORGEIRSSON, SS AF PADMANABHAN, R GIORDANO, T TAN, T THORGEIRSSON, SS TI IMMUNOMAGNETIC SEPARATION OF HYBRID COLONIES FOLLOWING CELL-FUSION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A885 EP A885 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702989 ER PT J AU PALMER, LD WALLACE, PK MUIRHEAD, KA HORAN, PK YANG, JC AF PALMER, LD WALLACE, PK MUIRHEAD, KA HORAN, PK YANG, JC TI THE REDISTRIBUTION OF TUMOR INFILTRATING LYMPHOCYTES IN MICE FOLLOWING ADOPTIVE IMMUNOTHERAPY SO FASEB JOURNAL LA English DT Meeting Abstract C1 ZYNAXIS CELL SCI INC,MALVERN,PA 19355. MED COLL PENN,DEPT MICROBIOL & IMMUNOL,PHILADELPHIA,PA 19129. NCI,SURG BRANCH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A639 EP A639 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701556 ER PT J AU PELINA, MD MACKAY, AR THORGEIRSSON, U AF PELINA, MD MACKAY, AR THORGEIRSSON, U TI DIFFERENTIAL EXPRESSION OF TUMOR-CELL METALLOPROTEINASES AND TIMPS BY A PHORBOL ESTER SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,DIV CANC ETIOL,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A594 EP A594 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701297 ER PT J AU PILARO, AM MILLER, ME MCCORMICK, KL SAYERS, TJ WILTROUT, RH AF PILARO, AM MILLER, ME MCCORMICK, KL SAYERS, TJ WILTROUT, RH TI CHEMOTACTIC ACTIVITY OF RECOMBINANT HUMAN CYTOKINES FOR RAT LARGE ANTIGRANULOCYTES LYMPHOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES FACIL,BRMP,EXPTL IMMUNOL LAB,FREDERICK,MD 21701. NCI,FREDERICK CANC RES FACIL,PRI DYNCORP,BCDP,FREDERICK,MD 21701. RI Sayers, Thomas/G-4859-2015 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A630 EP A630 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701505 ER PT J AU POLITINO, M VERES, Z TSAI, L STADTMAN, TC AF POLITINO, M VERES, Z TSAI, L STADTMAN, TC TI PARTIAL-PURIFICATION AND CHARACTERIZATION OF SELENO-TRANSFER RNA BIOSYNTHETIC ENZYME(S) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A438 EP A438 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700388 ER PT J AU POTISCHMAN, N BRINTON, LA LAIMING, V REEVES, WC AF POTISCHMAN, N BRINTON, LA LAIMING, V REEVES, WC TI SERUM FOLATE LEVELS AND INVASIVE CERVICAL-CANCER SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. MICROBIOL ASSOCIATES INC,ROCKVILLE,MD 20852. CTR DIS CONTROL,ATLANTA,GA 30333. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A562 EP A562 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701110 ER PT J AU RACIOPPI, L RONCHESE, F SCHWARTZ, R GERMAIN, R AF RACIOPPI, L RONCHESE, F SCHWARTZ, R GERMAIN, R TI QUANTITATIVE AND QUALITATIVE EFFECTS OF CLASS-II MHC MOLECULE POLYMORPHISM ON PEPTIDE BINDING SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A724 EP A724 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702052 ER PT J AU RAVUSSIN, E HARPER, I RISING, R FERRARO, R FONTVIEILLE, AM AF RAVUSSIN, E HARPER, I RISING, R FERRARO, R FONTVIEILLE, AM TI HUMAN OBESITY IS ASSOCIATED WITH LOWER LEVELS OF PHYSICAL-ACTIVITY - RESULTS FROM DOUBLY-LABELED WATER AND GAS EXCHANGES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,CLIN DIABET & NUTR SECT,PHOENIX,AZ 85016. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A554 EP A554 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701065 ER PT J AU RHEE, SG PARK, DG CHAE, HZ SIM, SS AF RHEE, SG PARK, DG CHAE, HZ SIM, SS TI REGULATION OF PHOSPHOLIPASE-C ISOZYMES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A699 EP A699 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701912 ER PT J AU RIBEIRO, P KAUFMAN, S AF RIBEIRO, P KAUFMAN, S TI THE EFFECT OF TETRAHYDROBIOPTERIN ON THE STATE OF PHOSPHORYLATION OF TYROSINE-HYDROXYLASE IN RAT STRIATAL SYNAPTOSOMES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,NEUROCHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A457 EP A457 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700500 ER PT J AU RISING, R HARPER, I FERRARO, R FONTVIEILLE, AM RAVUSSIN, E AF RISING, R HARPER, I FERRARO, R FONTVIEILLE, AM RAVUSSIN, E TI EFFECT OF AGE ON FREE LIVING ENERGY-EXPENDITURE IN PIMA-INDIANS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,CLIN DIABET & NUTR SECT,PHOENIX,AZ 85016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A555 EP A555 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701072 ER PT J AU RIZZO, P TINELLO, C PUNTURIERI, A TANIUCHI, H AF RIZZO, P TINELLO, C PUNTURIERI, A TANIUCHI, H TI ANTIGEN-BINDING AND ANTIBODY MOTILITY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A805 EP A805 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702522 ER PT J AU RONDINONE, CM RODBARD, D AF RONDINONE, CM RODBARD, D TI PROGESTERONE STIMULATES THE DIFFERENTIATION OF 3T3-L1 PREADIPOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,THEORET & PHYS BIOL,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A414 EP A414 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700256 ER PT J AU SCHULZ, LO ALGER, S HARPER, I WILMORE, JH RAVUSSIN, E AF SCHULZ, LO ALGER, S HARPER, I WILMORE, JH RAVUSSIN, E TI ENERGY-EXPENDITURE OF ELITE FEMALE RUNNERS MEASURED BY RESPIRATORY CHAMBER AND DOUBLY-LABELED WATER SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,CLIN DIABET & NUTR SECT,PHOENIX,AZ 85016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A554 EP A554 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701067 ER PT J AU SHEARER, GM CLERICI, M KEAY, S BERZOFSKY, JA TACKET, CO AF SHEARER, GM CLERICI, M KEAY, S BERZOFSKY, JA TACKET, CO TI EXPOSURE TO HIV-1 DETECTED BY T-HELPER CELL RESPONSES BEFORE DETECTION OF INFECTION BY SERUM ANTIBODIES OR POLYMERASE CHAIN-REACTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. NCI,METAB BRANCH,BETHESDA,MD 20892. UNIV MARYLAND,SCH MED,DEPT MED,CTR VACCINE DEV,BALTIMORE,MD 21201. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A650 EP A650 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701622 ER PT J AU SMITH, CJ MEACCI, E MOVSESIAN, MA MANGANIELLO, VC AF SMITH, CJ MEACCI, E MOVSESIAN, MA MANGANIELLO, VC TI CYCLIC AMP-DEPENDENT PHOSPHORYLATION OF THE CGMP-INHIBITED LOW KM CAMP PHOSPHODIESTERASE (CGI PDE) IN HUMAN MYOCARDIUM SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,CELL METAB LAB,BETHESDA,MD 20892. UNIV UTAH,MED CTR,DIV CARDIOL,SALT LAKE CITY,UT 84132. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A426 EP A426 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700321 ER PT J AU SMITH, PB PHILPOT, RM TIANO, HF LANGENBACH, RJ AF SMITH, PB PHILPOT, RM TIANO, HF LANGENBACH, RJ TI RETROVIRAL MEDIATED EXPRESSION OF PULMONARY FLAVIN MONOXYGENASE AND CYTOCHROME-P-450 IVB1 SO FASEB JOURNAL LA English DT Meeting Abstract C1 WAKE FOREST UNIV,MED CTR,DEPT BIOCHEM,WINSTON SALEM,NC 27103. NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A477 EP A477 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700616 ER PT J AU SOERGEL, DG GUSOVSKY, F DALY, JW AF SOERGEL, DG GUSOVSKY, F DALY, JW TI GLIBENCLAMIDE INDUCES PI BREAKDOWN IN RINM5F CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,LBC,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A857 EP A857 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702831 ER PT J AU SUBAR, AF ZIEGLER, RG PATTERSON, B BLOCK, G URSIN, G AF SUBAR, AF ZIEGLER, RG PATTERSON, B BLOCK, G URSIN, G TI NUTRIENT INTAKE AND FOOD PATTERNS ASSOCIATED WITH A LOW-FAT DIET SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. RI Block, Gladys/E-3304-2010 NR 0 TC 1 Z9 1 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A563 EP A563 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701116 ER PT J AU SUGIHARA, S FUJIWARA, H SHEARER, GM AF SUGIHARA, S FUJIWARA, H SHEARER, GM TI THYROID-SPECIFIC CD8+ T-CELL LINE DERIVED FROM AUTOIMMUNE-THYROIDITIS LESIONS IN MICE DEPLETED OF CD5 BRIGHT T-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. OSAKA UNIV,SCH MED,OSAKA 553,JAPAN. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A606 EP A606 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701364 ER PT J AU SUHADOLNIK, RJ REICHENBACH, NL SOBOL, RW HITZGES, P PETERSON, DL STRAYER, DR HENRY, B ABLASHI, DV GILLESPIE, DH CARTER, WA AF SUHADOLNIK, RJ REICHENBACH, NL SOBOL, RW HITZGES, P PETERSON, DL STRAYER, DR HENRY, B ABLASHI, DV GILLESPIE, DH CARTER, WA TI BIOCHEMICAL DEFECTS IN THE 2-5A SYNTHETASE RNASE-L PATHWAY ASSOCIATED WITH CHRONIC FATIGUE SYNDROME (CFS) SO FASEB JOURNAL LA English DT Meeting Abstract C1 TEMPLE UNIV,HLTH SCI CTR,SCH MED,PHILADELPHIA,PA 19140. HAHNEMANN UNIV,PHILADELPHIA,PA 19102. UNIV NEVADA,SCH MED,RENO,NV 89557. NCI,BETHESDA,MD 20892. RI Sobol, Robert/E-4125-2013 OI Sobol, Robert/0000-0001-7385-3563 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A824 EP A824 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702632 ER PT J AU SZWEDA, LI STADTMAN, ER AF SZWEDA, LI STADTMAN, ER TI IRON CATALYZED OXIDATIVE MODIFICATION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE FROM LEUCONOSTOC-MESENTEROIDES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A886 EP A886 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702995 ER PT J AU TSANG, KY KASHMIRI, S MASCI, AM PRIMUS, FJ SCHLOM, J AF TSANG, KY KASHMIRI, S MASCI, AM PRIMUS, FJ SCHLOM, J TI HEAVY-CHAIN CONTRIBUTION TO ANTIGEN-BINDING AND EFFECTOR FUNCTION OF AN ANTI-HUMAN COLORECTAL-CARCINOMA MONOCLONAL-ANTIBODY (MAB) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A733 EP A733 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702108 ER PT J AU WARREN, JS JONES, ML YOSHIMURA, T AF WARREN, JS JONES, ML YOSHIMURA, T TI MONOCYTE CHEMOTACTIC ACTIVITY IN A RAT MODEL OF PULMONARY GRANULOMATOSIS SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MICHIGAN,SCH MED,DEPT PATHOL,ANN ARBOR,MI 48109. NCI,IMMUNOBIOL LAB,FREDERICK,MD 21701. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A517 EP A517 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700848 ER PT J AU WELSH, CF PRICE, SR TSUCHIYA, M STANLEY, SJ NIGHTINGALE, MS LEE, CM MOSS, J AF WELSH, CF PRICE, SR TSUCHIYA, M STANLEY, SJ NIGHTINGALE, MS LEE, CM MOSS, J TI MOLECULAR-CLONING, EXPRESSION, AND FUNCTIONAL-CHARACTERIZATION OF 3 CLASSES OF MAMMALIAN ADP-RIBOSYLATION FACTORS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,CELLULAR METAB LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A821 EP A821 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702619 ER PT J AU WHITE, RE ARMSTRONG, DL AF WHITE, RE ARMSTRONG, DL TI SOMATOSTATIN INCREASES CA2+-ACTIVATED K+ CURRENT IN METABOLICALLY-INTACT PITUITARY-TUMOR (GH4) CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,LCMP,RES TRIANGLE PK,NC 27709. NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A410 EP A410 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700231 ER PT J AU WIDEN, SG WILSON, SH AF WIDEN, SG WILSON, SH TI PURIFICATION AND CHARACTERIZATION OF A BETA-POLYMERASE PROMOTER BINDING-FACTOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BIOCHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A817 EP A817 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702595 ER PT J AU WINSLOW, S HENKART, P AF WINSLOW, S HENKART, P TI EXPRESSION OF GRANULE PROTEOGLYCAN CORE PROTEIN IN LYMPHOCYTES AND EXOCYTOSIS OF CHONDROITIN SULFATE PROTEOGLYCAN BY CTL SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A601 EP A601 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701339 ER PT J AU WINTER, C CARRENO, B TURNER, R KOENIG, S BIDDISON, W AF WINTER, C CARRENO, B TURNER, R KOENIG, S BIDDISON, W TI THE 45-POCKET OF HLA-A2.1 PLAYS A ROLE IN PRESENTATION OF INFLUENZA-VIRUS MATRIX PEPTIDE AND ALLOANTIGENS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINCDS,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A723 EP A723 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702047 ER PT J AU WIRTH, PJ LUO, L FUJIMOTO, Y THORGEIRSSON, SS MILLER, MJ OLSON, AD AF WIRTH, PJ LUO, L FUJIMOTO, Y THORGEIRSSON, SS MILLER, MJ OLSON, AD TI COMPUTERIZED, COMPREHENSIVE, DATABASE OF CELLULAR POLYPEPTIDES FROM RAT-LIVER EPITHELIAL (RLE) CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A883 EP A883 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702979 ER PT J AU XU, Y MACEDONIA, J PEARCE, EJ SHER, A CHEEVER, AW AF XU, Y MACEDONIA, J PEARCE, EJ SHER, A CHEEVER, AW TI DYNAMIC ANALYSIS OF TH1 AND TH2 CYTOKINE PRODUCTION BY SPLEEN-CELLS FROM MICE INFECTED WITH SCHISTOSOMA-JAPONICUM SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A546 EP A546 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701016 ER PT J AU YANAGISHITA, M AF YANAGISHITA, M TI METABOLIC LABELING OF GLYCOSYLPHOSPHATIDYLINOSITOL MEMBRANE ANCHOR STRUCTURE OF CELL-SURFACE HEPARAN-SULFATE PROTEOGLYCANS IN RAT OVARIAN GRANULOSA-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A839 EP A839 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702719 ER PT J AU YASUMURA, S KIEBZAK, CM GOTHELF, T SHORT, L SPEERS, R DILMANIAN, A JONES, K SPANNE, P SCHIDLOVSKY, G GLAROS, D AF YASUMURA, S KIEBZAK, CM GOTHELF, T SHORT, L SPEERS, R DILMANIAN, A JONES, K SPANNE, P SCHIDLOVSKY, G GLAROS, D TI CHANGES IN TOTAL-BODY CALCIUM, TOTAL-BODY PHOSPHORUS, AND SKELETAL MORPHOLOGY IN THE AGING RAT SO FASEB JOURNAL LA English DT Meeting Abstract C1 SUNY HLTH SCI CTR,DEPT PHYSIOL,BROOKLYN,NY 11203. BROOKHAVEN NATL LAB,UPTON,NY 11973. NIA,GRC,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A561 EP A561 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20701105 ER PT J AU ZAPF, J ELLIS, PD FITZHUGH, AL DUNLAP, RB AF ZAPF, J ELLIS, PD FITZHUGH, AL DUNLAP, RB TI COVALENT STRUCTURE OF THE INHIBITORY TERNARY COMPLEX FORMED BY THYMIDYLATE SYNTHASE, 5-FLUORODEOXYURIDYLATE AND N-5, N-15N-10-METHYLENETETRAHYDROFOLATE BY N-15N NUCLEAR-MAGNETIC-RESONANCE SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV S CAROLINA,COLUMBIA,SC 29208. NCI,FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21701. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A789 EP A789 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20702432 ER PT J AU ZHANG, J HERMAN, EH MCCLURE, HM CHADWICK, DP FERRANS, VJ AF ZHANG, J HERMAN, EH MCCLURE, HM CHADWICK, DP FERRANS, VJ TI CARDIOTOXICITY OF HUMAN RECOMBINANT INTERLEUKIN-2 (IL-2) IN RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 US FDA,WASHINGTON,DC 20204. NIH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 11 PY 1991 VL 5 IS 4 BP A507 EP A507 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC207 UT WOS:A1991FC20700791 ER PT J AU POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR AF POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR TI DINUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN C-FMS PROTOONCOGENE FOR THE CFS-1 RECEPTOR (CFS1R) SO NUCLEIC ACIDS RESEARCH LA English DT Note RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 3 TC 23 Z9 23 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD MAR 11 PY 1991 VL 19 IS 5 BP 1160 EP 1160 DI 10.1093/nar/19.5.1160-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FC143 UT WOS:A1991FC14300041 PM 1826950 ER PT J AU LATIF, F GLENN, GM TORY, K BRAUCH, H DELISIO, J HAMPSCH, K ORCUTT, ML ZBAR, B LERMAN, MI AF LATIF, F GLENN, GM TORY, K BRAUCH, H DELISIO, J HAMPSCH, K ORCUTT, ML ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB41 - 51 (D3S632) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,FREDERICK CANC RES & DEV CTR,IMMUNOL LAB,FT DETRICK,BLDG 560,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,FREDERICK,MD 21701. NR 1 TC 1 Z9 1 U1 1 U2 6 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD MAR 11 PY 1991 VL 19 IS 5 BP 1163 EP 1163 DI 10.1093/nar/19.5.1163-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FC143 UT WOS:A1991FC14300047 PM 1708497 ER PT J AU POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR AF POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR TI DINUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN CYSTEINE-PROTEINASE INHIBITOR PSEUDOGENE (CSTP1) SO NUCLEIC ACIDS RESEARCH LA English DT Note ID CYSTATIN GENE FAMILY RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 4 TC 5 Z9 5 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD MAR 11 PY 1991 VL 19 IS 5 BP 1164 EP 1164 DI 10.1093/nar/19.5.1164 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FC143 UT WOS:A1991FC14300048 PM 2020557 ER PT J AU LATIF, F GLENN, GM TORY, K BRAUCH, H DELISIO, J HAMPSCH, K ORCUTT, ML ZBAR, B LERMAN, MI AF LATIF, F GLENN, GM TORY, K BRAUCH, H DELISIO, J HAMPSCH, K ORCUTT, ML ZBAR, B LERMAN, MI TI A NEW POLYMORPHIC PROBE ON CHROMOSOME-3P - LAMBDA-LIB46-100 (D3S641) SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,FREDERICK CANC RES & DEV CTR,IMMUNOL LAB,FT DETRICK,BLDG 560,FREDERICK,MD 21701. FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,FREDERICK,MD 21701. NR 1 TC 0 Z9 0 U1 1 U2 6 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD MAR 11 PY 1991 VL 19 IS 5 BP 1170 EP 1170 DI 10.1093/nar/19.5.1170-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FC143 UT WOS:A1991FC14300061 PM 1673567 ER PT J AU MCMAHON, TF STEFANSKI, SA WILSON, RE BLAIR, PC CLARK, AM BIRNBAUM, LS AF MCMAHON, TF STEFANSKI, SA WILSON, RE BLAIR, PC CLARK, AM BIRNBAUM, LS TI COMPARATIVE ACUTE NEPHROTOXICITY OF SALICYLIC-ACID, 2,3-DIHYDROXYBENZOIC ACID, AND 2,5-DIHYDROXYBENZOIC ACID IN YOUNG AND MIDDLE-AGED FISCHER-344 RATS SO TOXICOLOGY LA English DT Article DE SALICYLIC ACID; 2,3-DIHYDROXYBENZOIC ACID; 2,5-DIHYDROXYBENZOIC ACID; NEPHROTOXICITY; AGING ID URINARY ENZYMES AB Experimental evidence suggests that the oxidative metabolites, 2,3- and 2,5-dihydroxybenzoic acid (DIOH) may be responsible for the nephrotoxicity of salicylic acid (SAL). In the present study, enzymuria in conjunction with glucose (GLU) and protein (PRO) excretion were used as endpoints to compare the relative nephrotoxicity of SAL with 2,3- and 2,5-DIOH. In addition, the effect of age on enzymuria and GLU and PRO excretion following treatment with SAL or 2,3- and 2,5-DIOH was investigated because the elderly are at greater risk for SAL-induced nephrotoxicity. Three and 12-month male Fischer 344 rats were administered either no treatment, vehicle, SAL, 2,3-DIOH, or 2,5-DIOH at 500 mg/kg p.o. in 5 ml/kg corn oil/DMSO (5:1). Effects of these treatments on functional integrity of renal tissue was assessed from 0-72 h after dosing by measurement of urinary creatinine, GLU, and PRO, as well as excretion of proximal and distal tubular renal enzymes. Enzymes measured as indicators of proximal tubular damage were N-acetyl-beta-glucosaminidase (NAG), gamma glutamyltransferase (GGT), alanine aminotransferase (ALT), and alkaline phosphatase (AP), while urinary lactate dehydrogenase (LD) and aspartate aminotransferase (AST) were measured as indicators of distal tubular damage. In comparison to 3-month vehicle-treated rats, 2,3- and 2,5-DIOH caused a significant increase between 0-8 h in excretion of urinary GLU and activities of AST, NAG, and LD, with peak effects occurring between 4-8 h. Toxic effects of either metabolite were not evident beyond 24 h, and toxicity of 2,5-DIOH was significantly greater in comparison to 2,3-DIOH. SAL treatment resulted in similar effects on enzymuria as well as GLU and PRO excretion, but peak effects did not occur until 16-24 h, and often persisted until 72 h after dosing. Maximal enzymuria in response to SAL treatment was significantly greater in 12- vs. 3-month rats for AST, NAG, and LD. In response to 2,3-DIOH treatment, the maximal response was significantly greater in 12- vs. 3-month rats for LD and AST, and for NAG in response to 2,5-DIOH treatment. The results of this study suggest that both 2,3- and 2,5-DIOH are nephrotoxic metabolities of SAL, but implicate 2,5-DIOH as the more potent nephrotoxic metabolite. The relative lack of an age effect for 2,3- and 2,5-DIOH vs. SAL supports the hypothesis [2] that age-related differences in biotransformation of SAL, and not increased tissue sensitivity to 2,3- or 2,5-DIOH, contribute to the age-related increase in susceptibility to SAL-induced nephrotoxicity. C1 US EPA,DIV ENVIRONM TOXICOL,MD-66,RES TRIANGLE PK,NC 27711. NIEHS,ENVIRONM TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. NIEHS,MOLEC TOXICOL,RES TRIANGLE PK,NC 27709. BURROUGHS WELLCOME CO,RES TRIANGLE PK,NC 27709. COMP SCI CORP,RES TRIANGLE PK,NC 27709. NR 14 TC 7 Z9 7 U1 1 U2 2 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PD MAR 11 PY 1991 VL 66 IS 3 BP 297 EP 311 DI 10.1016/0300-483X(91)90201-B PG 15 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA FG533 UT WOS:A1991FG53300006 PM 2011854 ER PT J AU UBOM, GA RHEE, SG HUNT, JB CHOCK, PB AF UBOM, GA RHEE, SG HUNT, JB CHOCK, PB TI DISTANCE CHANGES AT THE REGULATORY AND CATALYTIC SITES ON ESCHERICHIA-COLI GLUTAMINE-SYNTHETASE - A SPIN LABEL STUDY ON THE EFFECT OF SUBSTRATE(S) BINDING SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Article DE GLUTAMINE SYNTHETASE; SPIN LABEL; BINDING SITE; CATALYTIC SITE; (ESCHERICHIA-COLI) AB A spin-labeled ATP analogue, 2,2,6,6-tetramethylpiperidine-1-oxyl adenosine triphophosphate (Tempo-ATP) is used to adenylate Escherichia coli glutamine synthetase (L-glutamine: ammonia ligase (ADP-forming), EC 6.3.1.2). The Tempo adenylylated glutamine synthetase (Tempo-GS) exhibits similar catalytic properties, pH profile and inhibitor susceptibility as those of glutamine synthetase adenylylated with normal ATP. Using the spin label on the enzyme as a probe and employing the spin-spin interactions between the label probe and paramagnetic Mn2+, the distances from the nitroxyl moiety of the covalently bound Tempo-AMP to the two Mn2+ binding sites, n1 and n2 were determined. The n1 site is the structural site and n2 is located at the catalytic site. The distances from Mn2+ at n1 and n2 sites to the nitroxyl radical are 19 and 16 angstrom, respectively. Binding of the substrate, L-Glu, causes a protein conformational change which is reflected by the reduction of approximately 2 angstrom for the n1 to Tempo-AMP distance and lengthening of approximately 2 angstrom for the n2 to the Tempo-AMP distance. Addition of ATP to the Tempo-GS/L-Glu complex increases the distance between n1 and Tempo-AMP, and n2 and Tempo-AMP by 4 and 3 angstrom, respectively. C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. CATHOLIC UNIV AMER,DEPT CHEM,WASHINGTON,DC 20064. RP UBOM, GA (reprint author), UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND. NR 21 TC 1 Z9 1 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD MAR 8 PY 1991 VL 1077 IS 1 BP 91 EP 98 DI 10.1016/0167-4838(91)90530-D PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FD599 UT WOS:A1991FD59900013 PM 1672611 ER PT J AU KLAUSNER, RD SAMELSON, LE AF KLAUSNER, RD SAMELSON, LE TI T-CELL ANTIGEN RECEPTOR ACTIVATION PATHWAYS - THE TYROSINE KINASE CONNECTION SO CELL LA English DT Review ID CD4 RECEPTOR RP KLAUSNER, RD (reprint author), NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892, USA. NR 30 TC 482 Z9 484 U1 0 U2 1 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD MAR 8 PY 1991 VL 64 IS 5 BP 875 EP 878 DI 10.1016/0092-8674(91)90310-U PG 4 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FA940 UT WOS:A1991FA94000003 PM 1848158 ER PT J AU ROUAULT, TA STOUT, CD KAPTAIN, S HARFORD, JB KLAUSNER, RD AF ROUAULT, TA STOUT, CD KAPTAIN, S HARFORD, JB KLAUSNER, RD TI STRUCTURAL RELATIONSHIP BETWEEN AN IRON-REGULATED RNA-BINDING PROTEIN (IRE-BP) AND ACONITASE - FUNCTIONAL IMPLICATIONS SO CELL LA English DT Letter ID FERRITIN MESSENGER-RNA; RESPONSIVE ELEMENT; IDENTIFICATION; MECHANISM; SEQUENCES; CLUSTER C1 Scripps Res Inst, RES INST, LA JOLLA, CA 92037 USA. RP ROUAULT, TA (reprint author), NICHHD, CELL BIOL & METAB BRANCH, BETHESDA, MD 20892 USA. NR 20 TC 236 Z9 239 U1 3 U2 3 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD MAR 8 PY 1991 VL 64 IS 5 BP 881 EP 883 DI 10.1016/0092-8674(91)90312-M PG 3 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FA940 UT WOS:A1991FA94000005 PM 2001588 ER PT J AU BOOY, FP NEWCOMB, WW TRUS, BL BROWN, JC BAKER, TS STEVEN, AC AF BOOY, FP NEWCOMB, WW TRUS, BL BROWN, JC BAKER, TS STEVEN, AC TI LIQUID-CRYSTALLINE, PHAGE-LIKE PACKING OF ENCAPSIDATED DNA IN HERPES-SIMPLEX VIRUS SO CELL LA English DT Article ID CRYO-ELECTRON-MICROSCOPY; DOUBLE-STRANDED DNA; 3-DIMENSIONAL STRUCTURE; DEOXYRIBONUCLEIC ACID; SIMIAN VIRUS-40; RECONSTRUCTION; TYPE-1; CORE; BACTERIOPHAGES; HERPESVIRUSES AB The organization of DNA within the HSV-1 capsid has been determined by cryoelectron microscopy and image reconstruction. Purified C-capsids, which are fully packaged, were compared with A-capsids, which are empty. Unlike A-capsids, C-capsids show fine striations and punctate arrays with a spacing of approximately 2.6 nm. The packaged DNA forms a uniformly dense ball, extending radially as far as the inner surface of the icosahedral (T = 16) capsid shell, whose structure is essentially identical in A-capsids and C-capsids. Thus we find no evidence for the inner T = 4 shell previously reported by Schrag et al. to be present in C-capsids. Encapsidated HSV-1 DNA closely resembles that previously visualized in bacteriophages T4 and lambda, thus supporting the idea of a close parallelism between the respective assembly pathways of a major family of animal viruses (the herpesviruses) and a major family of bacterial viruses. C1 UNIV VIRGINIA,HLTH SCI CTR,CTR CANC,CHARLOTTESVILLE,VA 22908. UNIV VIRGINIA,HLTH SCI CTR,DEPT MICROBIOL,CHARLOTTESVILLE,VA 22908. NIH,DIV COMP RES & TECHNOL,BETHESDA,MD 20892. PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907. RP BOOY, FP (reprint author), NIAMSD,STRUCT BIOL RES LAB,BLDG 6,ROOM 114,BETHESDA,MD 20892, USA. FU NIGMS NIH HHS [R37 GM033050, GM33050, R01 GM033050, GM34036] NR 41 TC 208 Z9 209 U1 1 U2 6 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD MAR 8 PY 1991 VL 64 IS 5 BP 1007 EP 1015 DI 10.1016/0092-8674(91)90324-R PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FA940 UT WOS:A1991FA94000017 PM 1848156 ER PT J AU PRIOR, TI FITZGERALD, DJ PASTAN, I AF PRIOR, TI FITZGERALD, DJ PASTAN, I TI BARNASE TOXIN - A NEW CHIMERIC TOXIN COMPOSED OF PSEUDOMONAS EXOTOXIN-A AND BARNASE SO CELL LA English DT Article ID GENOMIC SEQUENCES; ESCHERICHIA-COLI; BARSTAR; CELLS; RIBONUCLEASE; IMMUNOTOXINS; AERUGINOSA; EXPRESSION; ANTIBODY; PROTEINS AB We have constructed a chimeric toxin composed of Pseudomonas exotoxin A (PE) and the extracellular ribonuclease of Bacillus amyloliquefaciens, barnase. The chimeric protein, termed PE-Bar, reacted with both anti-PE and anti-barnase antisera and had both ADP ribosylation and ribonuclease activities. The chimeric toxin was cytotoxic to the murine fibroblast cell line L929 and to a murine hybridoma resistant to PE. A mutant form of PE-Bar lacking ADP-ribosylating activity was still cytotoxic to L929 cells. Because treatment of cells prelabeled with [H-3]uridine resulted in a decrease in their RNA content, we conclude that this cytotoxic effect was due to the ribonuclease activity of barnase molecules that had been translocated to the cytosol. It is now possible to construct chimeric toxins with two or more enzymatic activities that can be delivered to the cytosol of the target cells. RP PRIOR, TI (reprint author), NCI,DIV CANC BIOL & DIAG,MOLEC BIOL LAB,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 26 TC 61 Z9 62 U1 0 U2 0 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD MAR 8 PY 1991 VL 64 IS 5 BP 1017 EP 1023 DI 10.1016/0092-8674(91)90325-S PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FA940 UT WOS:A1991FA94000018 PM 1900455 ER PT J AU OBRIEN, SJ MAYR, E AF OBRIEN, SJ MAYR, E TI BUREAUCRATIC MISCHIEF - RECOGNIZING ENDANGERED SPECIES AND SUBSPECIES SO SCIENCE LA English DT Editorial Material ID HYBRID ZONES C1 HARVARD UNIV,MUSEUM COMPARAT ZOOL,CAMBRIDGE,MA 02138. RP OBRIEN, SJ (reprint author), NCI,FREDERICK CANC RES & DEV CTR,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702, USA. NR 32 TC 227 Z9 241 U1 2 U2 27 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 8 PY 1991 VL 251 IS 4998 BP 1187 EP 1188 DI 10.1126/science.251.4998.1187 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA691 UT WOS:A1991FA69100030 PM 17799277 ER PT J AU OTTEN, GR GERMAIN, RN AF OTTEN, GR GERMAIN, RN TI SPLIT ANERGY IN A CD8+ T-CELL - RECEPTOR-DEPENDENT CYTOLYSIS IN THE ABSENCE OF INTERLEUKIN-2 PRODUCTION SO SCIENCE LA English DT Article ID LYMPHOCYTES-T; CLASS-I; ANTIGEN; CLONES; INDUCTION; ACTIVATION; RESPONSIVENESS; TOLERANCE; INVIVO; EVENTS AB Engagement of the antigen-specific receptor (TCR) of CD4+ T lymphocytes without a second (costimulatory) signal prevents the subsequent production of interleukin-2 (IL-2) by these cells. Because IL-2 is a key immunoregulatory lymphokine and is also produced by a subset of CD8+ T cells that are able to kill target cells, the effect of engaging the TCR of one such clone in the absence of costimulatory signals was examined. The capacity for TCR-dependent IL-2 production was lost, indicating comparable costimulator-dependent signaling requirements for IL-2 production in CD4+ and CD8+ T cells. However, TCR-mediated cytotoxicity was not impaired, implying that costimulation is required for only certain TCR-dependent effector functions. RP OTTEN, GR (reprint author), NIAID,LYMPHOCYTE BIOL SECT,IMMUNOL LAB,BETHESDA,MD 20892, USA. NR 27 TC 200 Z9 202 U1 0 U2 1 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 8 PY 1991 VL 251 IS 4998 BP 1228 EP 1231 DI 10.1126/science.1900952 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA691 UT WOS:A1991FA69100040 PM 1900952 ER PT J AU GOLDSCHMIDTCLERMONT, PJ KIM, JW MACHESKY, LM RHEE, SG POLLARD, TD AF GOLDSCHMIDTCLERMONT, PJ KIM, JW MACHESKY, LM RHEE, SG POLLARD, TD TI REGULATION OF PHOSPHOLIPASE-C-GAMMA-1 BY PROFILIN AND TYROSINE PHOSPHORYLATION SO SCIENCE LA English DT Article ID PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; BOVINE BRAIN; RECEPTOR AB Epidermal growth factor and platelet-derived growth factor can stimulate the production of the second messenger inositol trisphosphate in responsive cells, but the biochemical pathway for these signaling events has been uncertain because the reactions have not been reconstituted with purified molecules in vitro. A reconstitution is described that requires not only the growth factor, its receptor with tyrosine kinase activity, and the soluble phospholipase C-gamma-1, but also the small soluble actin-binding protein profilin. Profilin binds to the substrate phosphatidylinositol 4,5-bisphosphate and inhibits its hydrolysis by unphosphorylated phospholipase C-gamma-1. Phosphorylation of phospholipase C-gamma-1 by the epidermal growth factor receptor tyrosine kinase overcomes the inhibitory effect of profilin and results in an effective activation of phospholipase C-gamma-1. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV CARDIOL,BALTIMORE,MD 21205. NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. RP GOLDSCHMIDTCLERMONT, PJ (reprint author), JOHNS HOPKINS UNIV,SCH MED,DEPT CELL BIOL & ANAT,BALTIMORE,MD 21205, USA. FU NIGMS NIH HHS [GM-26338] NR 29 TC 488 Z9 490 U1 0 U2 6 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 8 PY 1991 VL 251 IS 4998 BP 1231 EP 1233 DI 10.1126/science.1848725 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA691 UT WOS:A1991FA69100041 PM 1848725 ER PT J AU NUSSINOV, R AF NUSSINOV, R TI THE ORDERING OF NUCLEOTIDES IN THE DNA - STRONG PYRIMIDINE-PURINE PATTERNS NEAR HOMOOLIGOMER TRACTS SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article ID BIOLOGICAL IMPLICATIONS; REPLICATION ORIGIN; SEQUENCE; CONFORMATION; CURVATURE; ADENINE C1 TEL AVIV UNIV,SACKLER FAC MED,INST MOLEC MED,IL-69978 TEL AVIV,ISRAEL. RP NUSSINOV, R (reprint author), NCI,MATH BIOL LAB,BLDG 10,RM 4B-56,BETHESDA,MD 20892, USA. NR 32 TC 4 Z9 4 U1 0 U2 1 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-5193 J9 J THEOR BIOL JI J. Theor. Biol. PD MAR 7 PY 1991 VL 149 IS 1 BP 21 EP 42 DI 10.1016/S0022-5193(05)80069-4 PG 22 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA FG539 UT WOS:A1991FG53900002 PM 1881144 ER PT J AU DENNING, DW FOLLANSBEE, SE SCOLARO, M NORRIS, S EDELSTEIN, H STEVENS, DA AF DENNING, DW FOLLANSBEE, SE SCOLARO, M NORRIS, S EDELSTEIN, H STEVENS, DA TI PULMONARY ASPERGILLOSIS IN THE ACQUIRED-IMMUNODEFICIENCY-SYNDROME SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID IMMUNE-DEFICIENCY SYNDROME; CHRONIC GRANULOMATOUS-DISEASE; INVASIVE ASPERGILLOSIS; SYNDROME AIDS; OPPORTUNISTIC INFECTIONS; BRONCHIAL ASPERGILLOSIS; PRIMARY CYTOMEGALOVIRUS; PATIENT; THERAPY; CRYPTOCOCCOSIS AB Background and Methods. Symptomatic pulmonary aspergillosis has rarely been reported in patients with the acquired immunodeficiency syndrome (AIDS). We describe the predisposing factors, the clinical and radiologic features, and the therapeutic outcomes in 13 patients with pulmonary aspergillosis, all of whom had human immunodeficiency virus (HIV) infection and 12 of whom had AIDS. Results. Pulmonary aspergillosis was detected a median of 25 months after the diagnosis of AIDS, usually following corticosteroid use, neutropenia, pneumonia due to other pathogens, marijuana smoking, or the use of broad-spectrum antibiotics. Two major patterns of disease were observed: invasive aspergillosis (in 10 patients) and obstructing bronchial aspergillosis (in 3). Cough and fever, the most common symptoms, tended to be insidious in onset in patients with invasive disease (median duration, 1.3 months before diagnosis). Breathlessness, cough, and chest pain predominated in the three patients with obstructing bronchial aspergillosis, who coughed up fungal casts. Radiologic patterns included upper-lobe cavitary disease (sometimes mistaken for tuberculosis), nodules, pleural-based lesions, and diffuse infiltrates, usually of the lower lobe. Transbronchial biopsies were usually negative, but positive cultures were obtained from bronchoalveolar-lavage fluid or percutaneous aspirates. Dissemination to other organs occurred in at least two patients, and direct invasion of extrapulmonary sites was seen in two others. The results of treatment with amphotericin B, itraconazole, or both were variable. Ten of the patients died a median of 3 months after the diagnosis (range, 0 to 12 months). Conclusions. Pulmonary aspergillosis is a possible late complication of AIDS; if diagnosed early, it may be treated successfully. C1 SANTA CLARA VALLEY MED CTR,DEPT MED,DIV INFECT DIS,751 S BASCOM AVE,SAN JOSE,CA 95128. ST VINCENTS MED CTR,LOS ANGELES,CA 90057. COMMUNITY HOSP,INDIANAPOLIS,IN. NIAID,MYCOSES STUDY GRP,BETHESDA,MD 20892. VET AFFAIRS HOSP,MARTINEZ,CA. SANTA CLARA VALLEY MED CTR,DEPT MED,DIV CLIN MICROBIOL,SAN JOSE,CA 95128. SANTA CLARA VALLEY MED CTR,DEPT PATHOL,SAN JOSE,CA 95128. CALIF INST MED RES,SAN JOSE,CA. STANFORD UNIV,MED CTR,SCH MED,DEPT MED,DIV INFECT DIS,STANFORD,CA 94305. INFECT DIS ASSOCIATES MED GRP,SAN FRANCISCO,CA. OI Denning, David/0000-0001-5626-2251 NR 39 TC 348 Z9 356 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 7 PY 1991 VL 324 IS 10 BP 654 EP 662 DI 10.1056/NEJM199103073241003 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA FA144 UT WOS:A1991FA14400003 PM 1994248 ER PT J AU UNDERWOOD, BA MILTON, RC AF UNDERWOOD, BA MILTON, RC TI VITAMIN-A AND CHILDHOOD MORTALITY - REPLY SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter RP UNDERWOOD, BA (reprint author), NEI,BETHESDA,MD 20892, USA. NR 3 TC 1 Z9 1 U1 1 U2 1 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 7 PY 1991 VL 324 IS 10 BP 695 EP 696 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA FA144 UT WOS:A1991FA14400013 ER PT J AU BREZIN, AP EQWUAGU, CE SILVEIRA, C THULLIEZ, P MARTINS, MC MAHDI, RM BELFORT, R NUSSENBLATT, RB AF BREZIN, AP EQWUAGU, CE SILVEIRA, C THULLIEZ, P MARTINS, MC MAHDI, RM BELFORT, R NUSSENBLATT, RB TI ANALYSIS OF AQUEOUS-HUMOR IN OCULAR TOXOPLASMOSIS SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter ID POLYMERASE C1 CLIN SILVEIRA,EREXIM,BRAZIL. INST PUERICULTURE PARIS,PARIS,FRANCE. ESCOLA PAULISTA MED SCH,BR-04023 SAO PAULO,BRAZIL. RP BREZIN, AP (reprint author), NEI,BETHESDA,MD 20892, USA. RI Belfort Jr, Rubens/E-2252-2012 OI Belfort Jr, Rubens/0000-0002-8422-3898 NR 6 TC 50 Z9 51 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 7 PY 1991 VL 324 IS 10 BP 699 EP 699 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA FA144 UT WOS:A1991FA14400025 PM 1994259 ER PT J AU RAUB, W AF RAUB, W TI COMBINED AGENTS ELIMINATE HUMAN HIV-1 FROM T-CELL CULTURES SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD MAR 6 PY 1991 VL 265 IS 9 BP 1075 EP 1075 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EZ474 UT WOS:A1991EZ47400005 PM 1995987 ER PT J AU RAUB, W AF RAUB, W TI NEW DRUG AMELIORATES EFFECTS OF ASTHMA AND ALLERGIC RHINITIS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BETHESDA,MD 20892, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD MAR 6 PY 1991 VL 265 IS 9 BP 1075 EP 1075 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EZ474 UT WOS:A1991EZ47400007 PM 1995987 ER PT J AU RAUB, W AF RAUB, W TI IMMUNOGENETIC DISTINCTION FOUND BETWEEN PAUCIARTICULAR AND POLYARTICULAR JUVENILE ARTHRITIS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD MAR 6 PY 1991 VL 265 IS 9 BP 1075 EP 1075 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EZ474 UT WOS:A1991EZ47400006 PM 1995987 ER PT J AU MULE, JJ JICHA, DL AEBERSOLD, PM TRAVIS, WD ROSENBERG, SA AF MULE, JJ JICHA, DL AEBERSOLD, PM TRAVIS, WD ROSENBERG, SA TI DISSEMINATED HUMAN-MALIGNANT MELANOMA IN CONGENITALLY IMMUNE-DEFICIENT (BG/NU/XID) MICE SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID TUMOR-INFILTRATING LYMPHOCYTES; SEVERE COMBINED IMMUNODEFICIENCY; SCID-HU MOUSE; NUDE-MICE; METASTATIC MELANOMA; CELLS; IMMUNOTHERAPY; INTERLEUKIN-2; CANCER; MODEL AB Congenitally immune-deficient bg/nu/xid (BNX) mice are severely compromised in their ability to mount T-cell, B-cell, and lymphokine-activated killer (LAK) cell responses. Successful engraftment of BNX mice with human hematopoietic stem cells has been demonstrated recently. We have investigated the potential use of BNX mice for studies relating to the biology and immunotherapy of human malignant melanoma. The intravenous injection of fresh single-cell suspensions of human malignant melanomas into mice resulted in widely disseminated disease. Metastatic spread of human melanoma in BNX mice mimicked that observed in patients: eg, there were numerous tumor nodules identified in the subcutaneous tissues as well as in a variety of visceral organs, including spleen, kidneys, thyroid, adrenals, lungs, heart, and brain. BNX mouse lymph nodes were replaced consistently by human malignant melanoma cells. The presence of human tumor cells in these mice was confirmed by histologic analysis and microcytofluorometry analyses using human melanoma-specific monoclonal antibodies (MAbs). Moreover, human melanoma cells passaged in BNX mice remained lysable in vitro by specifically cytolytic, autologous human tumor-infiltrating lymphocytes (TILs). The capacity of fresh human malignant melanoma to disseminate widely in BNX mice may prove valuable not only for study of the biology of metastatic spread but also for studies of the immunotherapy of human melanoma using melanoma-specific MAbs and chemotherapeutic agents, as well as human TILs and LAK cells with or without retrovirus-mediated gene transfer modification. C1 NCI,PATHOL LAB,BETHESDA,MD 20892. RP MULE, JJ (reprint author), NCI,SURG BRANCH,BLDG 10,RM 2B42,BETHESDA,MD 20892, USA. NR 32 TC 20 Z9 20 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD MAR 6 PY 1991 VL 83 IS 5 BP 350 EP 355 DI 10.1093/jnci/83.5.350 PG 6 WC Oncology SC Oncology GA EZ953 UT WOS:A1991EZ95300013 PM 1995918 ER PT J AU DEVESA, SS BLOT, WJ FRAUMENI, JF AF DEVESA, SS BLOT, WJ FRAUMENI, JF TI DECLINING RATES OF LUNG-CANCER IN THE UNITED-STATES IN YOUNG MEN AND WOMEN - RESPONSE SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Letter RP DEVESA, SS (reprint author), NCI,DIV CANC ETIOL,EPIDEMIOL & BIOSTAT PROGRAM,EXECUT PLAZA N,ROOM 415,BETHESDA,MD 20892, USA. NR 4 TC 0 Z9 0 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD MAR 6 PY 1991 VL 83 IS 5 BP 369 EP 370 DI 10.1093/jnci/83.5.369 PG 2 WC Oncology SC Oncology GA EZ953 UT WOS:A1991EZ95300019 ER PT J AU CLORE, GM WINGFIELD, PT GRONENBORN, AM AF CLORE, GM WINGFIELD, PT GRONENBORN, AM TI HIGH-RESOLUTION 3-DIMENSIONAL STRUCTURE OF INTERLEUKIN-1-BETA IN SOLUTION BY 3-DIMENSIONAL AND 4-DIMENSIONAL NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY SO BIOCHEMISTRY LA English DT Article ID RESTRAINED MOLECULAR-DYNAMICS; RECEPTOR-BINDING AFFINITY; DISTANCE GEOMETRY; NMR-SPECTROSCOPY; MUTANT PROTEINS; ASSIGNMENT; RESIDUES; H-1-NMR AB The determination of the high-resolution three-dimensional solution structure of interleukin 1-beta (IL-1-beta), a protein of 153 residues and 17.4 kDa, which plays a central role in the immune and inflammatory responses, has been determined by heteronuclear (C-13 and N-15) three- and four-dimensional NMR spectroscopy. The structure is based on 3146 experimental restraints comprising 2780 distance and 366 torsion angle (phi, psi, and chi-1) restraints. A total of 32 simulated annealing structures are calculated, and the atomic RMS distribution about the mean coordinate positions is 0.41 +/- 0.04 angstrom for the backbone atoms and 0.82 +/- 0.04 angstrom for all atoms (excluding residue 1 at the N-terminus and residues 152 and 153 at the C-terminus, which are partially disordered). In the case of internal side chains with a surface accessibility of less-than-or-equal-to 40%, the atomic RMS distribution about the mean coordinate positions for all atoms is 0.49 +/- 0.03 angstrom. IL-1-beta resembles a tetrahedron and is composed of 12-beta-strands arranged in three pseudosymmetric topological units, each of which comprises 5 strands. Analysis of the mutational data on IL-1-beta in the light of the three-dimensional structure suggests the presence of three distinct binding sites for the IL-1 receptor on the surface of the protein. It is suggested that each of the three immunoglobulin domains which comprise the extracellular portion of the IL-1 receptor recognizes one of these sites. C1 NIAID,PROT EXPRESS LAB,BLDG 6B,BETHESDA,MD 20892. RP CLORE, GM (reprint author), NIAID,CHEM PHYS LAB,BLDG 2,BETHESDA,MD 20892, USA. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 46 TC 168 Z9 170 U1 0 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 5 PY 1991 VL 30 IS 9 BP 2315 EP 2323 DI 10.1021/bi00223a005 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FA348 UT WOS:A1991FA34800005 PM 2001363 ER PT J AU HYLDEN, JLK THOMAS, DA IADAROLA, MJ NAHIN, RL DUBNER, R AF HYLDEN, JLK THOMAS, DA IADAROLA, MJ NAHIN, RL DUBNER, R TI SPINAL OPIOID ANALGESIC EFFECTS ARE ENHANCED IN A MODEL OF UNILATERAL INFLAMMATION HYPERALGESIA - POSSIBLE INVOLVEMENT OF NORADRENERGIC MECHANISMS SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Article DE SPINAL CORD; OPIOIDS; CARRAGEENAN; THERMAL HYPERALGESIA; ANTINOCICEPTION; ALPHA-2-ADRENOCEPTORS; (RAT); (INTRATHECAL) ID ADJUVANT-INDUCED ARTHRITIS; OPIATE RECEPTORS; KAPPA-OPIATE; MU-OPIATE; SELECTIVE AGONIST; GENE-EXPRESSION; LOCALIZED PAIN; CORD DYNORPHIN; SUBSTANCE-P; RATS AB We have examined the spinal analgesic activity of opioid agonists and antagonists in a model of short term, unilateral, carrageenan-induced inflammation/hyperalgesia. Rats received a single s.c. injection of carrageenan (2-6 mg in saline) 3-24 h prior to testing hindpaw withdrawal latencies to noxious thermal stimuli. Dose-response curves for intrathecally administered agonists with mu- and/or delta-opioid activity were shifted to the left for inflamed hindpaws when compared to contralateral non-inflamed paws. The selective kappa-receptor agonist U-50,488H had no activity in this analgesic assay on either inflamed or non-inflamed paws when administered intrathecally. However, systemic administration of U-50,488H did produce significant elevations of paw withdrawal latencies in inflamed paws. The alpha-2-adrenoceptor agonist clonidine also produced dose-dependent antinociception in the paw withdrawal assay after systemic or intrathecal administration. Inflamed hindpaws were significantly more sensitive to the antinociceptive effects of clonidine than were non-inflamed paws. The antinociceptive effect of morphine on inflamed hindpaws was blocked by the opioid antagonist naloxone or the alpha-2-adrenoceptor antagonist idazoxan. The effect of clonidine was only blocked by idazoxan. Antagonists alone had no significant effect on withdrawal latencies. The data indicate that the analgesic action of opioids during conditions of inflammation may depend on an interaction with spinal noradrenergic pathways. RP HYLDEN, JLK (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 30,ROOM B-20,BETHESDA,MD 20892, USA. NR 50 TC 184 Z9 186 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD MAR 5 PY 1991 VL 194 IS 2-3 BP 135 EP 143 DI 10.1016/0014-2999(91)90097-A PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FD473 UT WOS:A1991FD47300001 PM 1676373 ER PT J AU BALDINI, G HOHMAN, R CHARRON, MJ LODISH, HF AF BALDINI, G HOHMAN, R CHARRON, MJ LODISH, HF TI INSULIN AND NONHYDROLYZABLE GTP ANALOGS INDUCE TRANSLOCATION OF GLUT-4 TO THE PLASMA-MEMBRANE IN ALPHA-TOXIN-PERMEABILIZED RAT ADIPOSE-CELLS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Note ID RESPONSIVE GLUCOSE TRANSPORTER; ADRENAL CHROMAFFIN CELLS; 3T3-L1 ADIPOCYTES; CATECHOLAMINE SECRETION; GUANINE-NUCLEOTIDES; MESSENGER-RNA; G-PROTEINS; CLONING; TISSUES; SEQUENCE AB Rat adipose cells treated with Staphylococcus aureus alpha-toxin are permeable and retain their ability to respond to insulin after hormone treatment. The GLUT 4 glucose transporter isoform, specific to fat and muscle cells, is translocated normally from low density microsomes to the plasma membrane in permeabilized cells. Addition of guanosine 5'-O-(3-thiotriphosphate), guanylyl imidodiphosphate, or guanylyl beta, gamma-methylenediphosphate to permeabilized adipocytes induces an insulin-like translocation of GLUT 4 to the plasma membrane; GTP or adenosine 5'-(beta, gamma-imino)triphosphate has no effect. No translocation of GLUT 4 is observed when GTP analogs are added to intact adipocytes. These results suggest the involvement of a GTP-binding protein in insulin-triggered recruitment of GLUT 4 to the cell surface. C1 NIAID,BETHESDA,MD 20892. MIT,DEPT BIOL,CAMBRIDGE,MA 02139. RP BALDINI, G (reprint author), WHITEHEAD INST BIOMED RES,CAMBRIDGE,MA 02142, USA. FU NHLBI NIH HHS [HL41484]; NIDDK NIH HHS [DK08101]; NIGMS NIH HHS [GM40916] NR 40 TC 145 Z9 145 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 5 PY 1991 VL 266 IS 7 BP 4037 EP 4040 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FA694 UT WOS:A1991FA69400004 PM 1999400 ER PT J AU WELLHONER, HH NEVILLE, DM SRINIVASACHAR, K ERDMANN, G AF WELLHONER, HH NEVILLE, DM SRINIVASACHAR, K ERDMANN, G TI UPTAKE AND CONCENTRATION OF BIOACTIVE MACROMOLECULES BY K562 CELLS VIA THE TRANSFERRIN CYCLE UTILIZING AN ACID-LABILE TRANSFERRIN CONJUGATE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID DIPHTHERIA-TOXIN; P-GLYCOPROTEIN; RECEPTOR; TRANSPORT; INTERNALIZATION; FRAGMENT; AGENTS; ENTRY AB The transferrin cycle was used to attempt the import of bioactive macromolecules into cells with the aid of an acid-labile cross-linking agent. Anti-tetanus F(ab')2 fragments were iodinated and then conjugated to transferrin with a newly developed acid-labile cleavable cross-linking reagent, bismaleimidoethoxy propane, following thiolation of both proteins. Noncleavable conjugates were also prepared. At saturating conjugate concentrations, the uptake rate for both conjugates averaged over the first 2 h is about 6.5 fmol/million cells/min. Incubation of loaded cells in fresh medium for 30 min and analysis of cell pellets and supernatants reveal that 1) of the previously cell-associated label, only intact conjugate (about 50% of the label) is returned to the medium; 2) most of the remaining cell-associated material for the cleavable conjugate is chromatographically coincident with free Fab with some contribution from free F(ab')2 fragments. In contrast, the cell pellets loaded with noncleavable conjugates contained intact transferrin-F(ab')2 conjugates. These results are consistent with transferrin receptor-mediated uptake of acid-labile conjugate followed by hydrolysis in acidified endosomes and resulting in concentration of free F(ab')2 and Fab within a prelysosomal intracellular compartment. A protein shuttle such as transferrin may therefore be used with ketal based acid-labile cross-linkers to load foreign molecules into an intracellular compartment. In addition, these data provide independent confirmation of the low pH compartment within the transferrin cycle. This new methodology is applicable to other cases of receptor/ligand trafficking to report low pH compartments independent of morphological analysis. Since transferrin receptors are overexpressed in tumors, antineoplastic agents could be targeted to tumors as transferrin acid-labile conjugates. This import system might be particularly useful in combatting the tumor cell export of antitumor agents occurring in multidrug resistance. C1 NIMH,MOLEC BIOL LAB,BIOPHYS CHEM SECT,BLDG 36,RM 1B-08,BETHESDA,MD 20892. MED SCH HANNOVER,DEPT TOXICOL,HANNOVER,GERMANY. NR 24 TC 22 Z9 22 U1 0 U2 7 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 5 PY 1991 VL 266 IS 7 BP 4309 EP 4314 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FA694 UT WOS:A1991FA69400047 PM 1671860 ER PT J AU STEENKAMP, HJ JOOSTE, PL JORDAAN, PCJ SWANEPOEL, ASP ROSSOUW, JE AF STEENKAMP, HJ JOOSTE, PL JORDAAN, PCJ SWANEPOEL, ASP ROSSOUW, JE TI CHANGES IN SMOKING DURING A COMMUNITY-BASED CARDIOVASCULAR-DISEASE INTERVENTION PROGRAM - THE CORONARY RISK FACTOR STUDY SO SOUTH AFRICAN MEDICAL JOURNAL LA English DT Article ID CORONARY HEART-DISEASE; TRIAL; MORTALITY; CIGARETTE; PROJECT; MEN AB A prospective anti-smoking clinical trial was conducted as part of a coronary risk factor intervention study in three rural South African communities in the south-western Cape over a period of 4 years. The aim of this part of the study was to reduce smoking rates in two of the communities through application of high- and low-intensity intervention. The effect was evaluated by examining the net change in smoking habits, which was defined as the residual change in the intervention areas after allowing for change in the reference area. This paper presents the analyses of the estimated effect of the programme on the cohort aged 15 - 64 years at baseline who participated in the two surveys (4087 subjects). The intervention programme among men in the high-intensity intervention area resulted in a reduction of 8,4% in smoking rates and 13,0% in the amount smoked per day. Among women in this area there was a reduction of 30,6% in smoking rates and 20,5% in amount smoked. Smoking and the amount smoked per day also decreased in the low-intensity intervention area, but less so than in the high-intensity intervention area. Smoking quilt rates were strongly associated with initial smoking levels, with light smokers being significantly more successful quitters than heavy smokers. This study has proved that a community-based intervention programme can effectively reduce smoking. C1 HUMAN SCI RES COUNCIL,INST COMMUN RES,PRETORIA,SOUTH AFRICA. HOUSE ASSEMBLY,DEPT HLTH SERV & WELF,PRETORIA,SOUTH AFRICA. NHLBI,LIPID METAB ATHEROGENESIS BRANCH,BETHESDA,MD 20892. RP STEENKAMP, HJ (reprint author), S AFRICAN MRC,INST NUTR DIS,JOHANNESBURG 2000,SOUTH AFRICA. NR 15 TC 8 Z9 8 U1 0 U2 0 PU MED ASSOC S AFRICA PI JOHANNESBURG PA MED HOUSE CENTRAL SQ 7430 PINELANDS JOHANNESBURG, SOUTH AFRICA SN 0038-2469 J9 S AFR MED J JI S. Afr. Med. J. PD MAR 2 PY 1991 VL 79 IS 5 BP 250 EP 253 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA FA665 UT WOS:A1991FA66500008 PM 2011802 ER PT J AU GENAZZANI, AD RODBARD, D AF GENAZZANI, AD RODBARD, D TI USE OF THE RECEIVER OPERATING CHARACTERISTIC CURVE TO EVALUATE SENSITIVITY, SPECIFICITY, AND ACCURACY OF METHODS FOR DETECTION OF PEAKS IN HORMONE TIME-SERIES SO ACTA ENDOCRINOLOGICA LA English DT Article ID LUTEINIZING-HORMONE; DETECTION ALGORITHMS; POSITIVE ACCURACY; PULSE-DETECTION; MEN; SECRETION; RELEASE; SIGNALS AB We utilize the "Receiver Operating Characteristic" to describe the relationship between sensitivity and specificity as the threshold for peak detection is varied systematically, to provide objective comparison of the performance of methods for detection of episodic hormonal secretion. A computer program was used to generate synthetic data with peaks with variable durations, with constant or variable height, shape and/or interpulse interval. This approach was used to compare the CLUSTER and DETECT programs. For both programs, the observed false positive rates estimated using signal-free data were in good agreement with the nominal rates, but in the presence of signal the observed false positive rates were systematically lower. Sensitivity increases with increasing signal/noise ratio, as expected. Program DETECT, using its standard options, provided excellent sensitivity (90-100%) with very low false positive rate under all conditions tested. Its performance could be further improved by the use of a more stringent definition of a peak requiring the presence of "UP" followed by a "DOWN". The CLUSTER program was found to have very poor sensitivity when using the "local variance" option. Use of the true fixed standard deviation or percent coefficient of variation resulted in a modest improvement. Optimal performance of program CLUSTER was obtained by the use of the best of 3 variance models, testing 12 different cluster sizes (from 1 x 1 to 4 x 4) and selecting the best among these: under these conditions it can achieve high sensitivity (90-100%) for very low observed false positive rate, such that its performance was comparable to that of DETECT. The methods developed and illustrated here should permit the definitive characterization and validation of the performance of any one method, the objective comparison of the relative performance of two or more methods for analysis of pulsatile hormone levels for episodic hormone secretion, and lead to the improvement of algorithms for peak detection. C1 NICHHD,THEORET & PHYS BIOL LAB,BLDG 10,ROOM 6C 101,BETHESDA,MD 20892. NR 19 TC 36 Z9 36 U1 0 U2 1 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0001-5598 J9 ACTA ENDOCRINOL-COP JI Acta Endocrinol. PD MAR PY 1991 VL 124 IS 3 BP 295 EP 306 PG 12 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FE480 UT WOS:A1991FE48000012 PM 2011919 ER PT J AU CASTILLO, JL CARTIER, L ARAYA, F VERDUGO, R MORA, C GIBBS, C AF CASTILLO, JL CARTIER, L ARAYA, F VERDUGO, R MORA, C GIBBS, C TI EVOKED-POTENTIAL ABNORMALITIES IN PROGRESSIVE SPASTIC PARAPARESIS ASSOCIATED TO HTLV-1 SO ACTA NEUROLOGICA SCANDINAVICA LA English DT Article DE RETROVIRUS INFECTION; ELECTROPHYSIOLOGICAL STUDY; EVOKED POTENTIALS ID I-ASSOCIATED MYELOPATHY AB The electrophysiological features of progressive spastic paraparesis (PSP) associated with HTLV-1 in Chile, a non-tropical country, are presented. Twenty-two of 45 PSP subjects were positive for HTLV-1 antibodies. Trimodal evoked potentials were all normal in only 2 of these cases (9.1%). Somatosensory evoked potentials (SSEPs) were abnormal in 19 patients (86.3%) with a mean amplitude of 1.31 uV SD 0.92. Visual evoked potentials (VEPs) and brainstem auditory evoked potentials (BAEPs) were normal in all patients except four. Peripheral nerve conduction was normal in all but one who showed discrete slowness of the motor conduction velocity in the peroneal nerves. EMG was normal in 15 cases in whom it was performed. SSEP's were abnormal in 2 (8.7%) of 23 HTLV-1 negative cases with a mean amplitude of 2.4 uV SD 1.5, which is statistically different with respect to the positive cases (p < 0.001). These results support an involvement of the spinal cord not restricted to the pyramidal tracts but also including subclinical damage of the posterior columns in PSP associated to HTLV-1. C1 NIH,CENT NERVOUS SYST STUDIES LAB,BETHESDA,MD 20892. RP CASTILLO, JL (reprint author), UNIV CHILE,DEPT NEUROL SCI,JM INFANTE 553,SANTIAGO,CHILE. NR 16 TC 19 Z9 20 U1 0 U2 0 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0001-6314 J9 ACTA NEUROL SCAND JI Acta Neurol. Scand. PD MAR PY 1991 VL 83 IS 3 BP 151 EP 154 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA FC203 UT WOS:A1991FC20300002 PM 2031447 ER PT J AU GRUSKY, O THOMPSON, WA TILLIPMAN, H AF GRUSKY, O THOMPSON, WA TILLIPMAN, H TI CLINICAL VERSUS ADMINISTRATIVE BACKGROUNDS FOR MENTAL-HEALTH ADMINISTRATORS SO ADMINISTRATION AND POLICY IN MENTAL HEALTH LA English DT Article C1 UNIV MISSOURI,HLTH SERV MANAGEMENT,COLUMBIA,MO 65201. MENTAL HLTH SERV N CENT,CINCINNATI,OH 45237. RP GRUSKY, O (reprint author), UNIV CALIF LOS ANGELES,DEPT WHO,NIMH,RES TRAINING PROGRAM,405 HILGARD AVE,LOS ANGELES,CA 90024, USA. NR 44 TC 2 Z9 2 U1 0 U2 0 PU HUMAN SCI PRESS INC PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013-1578 SN 0894-587X J9 ADM POLICY MENT HLTH JI Adm. Policy. Ment. Health PD MAR PY 1991 VL 18 IS 4 BP 271 EP 278 DI 10.1007/BF00706051 PG 8 WC Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA FE111 UT WOS:A1991FE11100007 ER PT J AU SHEARER, GM CLERICI, M AF SHEARER, GM CLERICI, M TI EARLY T-HELPER CELL DEFECTS IN HIV-INFECTION SO AIDS LA English DT Review DE HIV; AIDS; ASYMPTOMATIC; T-HELPER CELLS; ANTIGEN-PRESENTING CELLS; T-HELPER CELL DEFECTS; CD4; SELF-RESTRICTION ID HUMAN IMMUNODEFICIENCY VIRUS; IMMUNE-DEFICIENCY SYNDROME; HUMAN PERIPHERAL-BLOOD; EPSTEIN-BARR VIRUS; MIXED LYMPHOCYTE-REACTION; LYMPHOTROPIC VIRUS; SYNDROME AIDS; IMMUNOSUPPRESSIVE FACTORS; MONONUCLEAR PHAGOCYTES; MONOCLONAL-ANTIBODIES RP SHEARER, GM (reprint author), NCI, EXPTL IMMUNOL BRANCH, BLDG 10, ROOM 4B-17, BETHESDA, MD 20892 USA. NR 88 TC 169 Z9 169 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0269-9370 EI 1473-5571 J9 AIDS JI Aids PD MAR PY 1991 VL 5 IS 3 BP 245 EP 253 DI 10.1097/00002030-199103000-00001 PG 9 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA FG423 UT WOS:A1991FG42300001 PM 1676274 ER PT J AU KOLIDA, K UKAI, H BALZARINI, J DECLERCQ, E TORRENCE, PF AF KOLIDA, K UKAI, H BALZARINI, J DECLERCQ, E TORRENCE, PF TI DIFFERENTIAL INHIBITION OF RETROVIRAL REVERSE-TRANSCRIPTASE BY POLY(2-FLUOROADENYLIC ACID), A TEMPLATE ANALOG SO AIDS LA English DT Letter C1 CATHOLIC UNIV LEUVEN,REGA INST,B-3000 LOUVAIN,BELGIUM. NIDDKD,MED CHEM LAB,BIOMED CHEM SECT,BETHESDA,MD. RP KOLIDA, K (reprint author), NAGOYA CITY UNIV,FAC PHARMACEUT SCI,TANABE DORI,MIZUHO KU,NAGOYA,AICHI 467,JAPAN. NR 12 TC 0 Z9 0 U1 0 U2 2 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0269-9370 J9 AIDS JI Aids PD MAR PY 1991 VL 5 IS 3 BP 341 EP 341 PG 1 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA FG423 UT WOS:A1991FG42300020 ER PT J AU GOTO, Y YEH, CK NOTKINS, AL PRABHAKAR, BS AF GOTO, Y YEH, CK NOTKINS, AL PRABHAKAR, BS TI DETECTION OF PROVIRAL SEQUENCES IN SALIVA OF PATIENTS INFECTED WITH HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID PERIPHERAL-BLOOD; HTLV-III; T-CELL; AIDS; TRANSMISSION; HIV-1; RISK; LYMPHOCYTES; CHILDREN; MEN AB Single samples of saliva collected from 20 human immunodeficiency virus type I (HIV-1) seropositive patients were tested by the polymerase chain reaction for HIV-1 proviral sequences using primers from the long terminal repeat (LTR), gag, and env regions of the virus. Proviral sequences were detected in the saliva of 50% of the patients. Sequential samples of saliva, collected at four different times, from each of six additional patients led to the detection of proviral sequences in 100% of the patients. Since, however, the detection of HIV-1 required not only the highly sensitive polymerase chain reaction, but also multiple samples, it appears that under ordinary circumstances infected cells are present in saliva in low numbers. Although this may explain the lack of transmission of HIV-1 by casual contact through the salivary route to household members and health-care workers, the presence of infected cells in the saliva of a high percentage of patients argues for avoidance of sexually intimate situations involving prolonged and repeated contact with saliva. C1 NIDR,ORAL MED LAB,BLDG 30,ROOM 121,BETHESDA,MD 20892. NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892. NR 23 TC 49 Z9 49 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD MAR PY 1991 VL 7 IS 3 BP 343 EP 347 DI 10.1089/aid.1991.7.343 PG 5 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA FC323 UT WOS:A1991FC32300013 PM 2064831 ER PT J AU MICKISCH, G BIER, H TSCHADA, R ALKEN, P AF MICKISCH, G BIER, H TSCHADA, R ALKEN, P TI CHEMORESISTANCE OF RENAL-CELL CARCINOMA - A POSSIBLE MODULATION BY BUTHIONINE SULFOXIMINE MEDIATED GLUTATHIONE DEPLETION SO AKTUELLE UROLOGIE LA German DT Article DE HUMAN RENAL CELL CARCINOMA; CHEMORESISTANCE; BUTHIONINE SULFOXIMINE; MTT STAINING ASSAY AB Defined resistance factors play a substantial role in the characterization of chemoresistance. The glutathione metabolism is apparently involved in the expression of high chemoresistance in human renal cell carcinoma. In 35 renal cell carcinomas, primary monolayer cultures were generated, the glutathione content enzymatically determined and the glutathione depletion instigated by buthionine sulfoximine, a specific inhibitor of the glutathione biosynthesis, was studied. Furthermore, the degree of chemoresistance against doxorubicin and carboplatinum was determined in an MTT microculture assay and a possible modulation of resistance by buthionine sulfoximine was examined. The glutathione content amounted to 81 +/- 26 nM/mg protein. A high degree of chemoresistance was associated with increased levels, a low degree with reduced values. Applying culture conditions, a reduction of the glutathione content to approx. 30% of the basic value could be achieved. By that, a distinct potentiation of carboplatinum cytotoxicity could be detected and doxorubicin resistance was still measurably subdued. RP MICKISCH, G (reprint author), NCI,MOLEC BIOL LAB,BLDG 37,RM 2D27,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORG THIEME VERLAG PI STUTTGART PA P O BOX 30 11 20, D-70451 STUTTGART, GERMANY SN 0001-7868 J9 AKTUEL UROL JI Aktuelle Urol. PD MAR PY 1991 VL 22 IS 2 BP 79 EP 84 DI 10.1055/s-2008-1060483 PG 6 WC Urology & Nephrology SC Urology & Nephrology GA FK003 UT WOS:A1991FK00300005 ER PT J AU MICKISCH, G MERLINO, GT GALSKI, H WILLINGHAM, MC GOTTESMAN, MM PASTAN, I AF MICKISCH, G MERLINO, GT GALSKI, H WILLINGHAM, MC GOTTESMAN, MM PASTAN, I TI INVIVO EXAMINATION OF THE HUMAN MDR-1 GENE USING TRANSGENIC MICE SO AKTUELLE UROLOGIE LA German DT Article DE HUMAN MULTIDRUG RESISTANCE GENE-1; TRANSGENIC MICE; INVIVO MODEL; TAXOL; R-VERAPAMIL AB Multidrug resistance (MDR) encoded by the human MDR-1 gene instigates a broad spectrum cross-chemoresistance in a multitude of human tumors including renal cell carcinoma. The development of preclinical models for the in vivo examination of MDR is a high priority of oncological research. We have engineered transgenic mice, derived from C57B1/6 x SLJL F1 strains, carrying the human MDR-1 gene under control of a chicken beta-actin promoter. The transgene was inherited in a dominant mendelian fashion. Expression was mainly detected in hematopoietic tissues such as bone marrow and spleen. Immunofluorescence studies traced P-170 glycoprotein in bone marrow indicating translation of the transgene into protein. Since we could demonstrate that MDR-transgenic mice were selectively resistant to leukopenia induced by all MDR-related drugs, functional activity of the transgene was ascertained. In addition, we could show that the resistance conferred by the MDR-1 gene was circumvented in vivo in a dose dependent manner by simultaneous administration of reversing agents. We conclude that MDR-transgenic mice provide a suitable in vivo system to examine MDR. RP MICKISCH, G (reprint author), NCI,MOLEC BIOL LAB,BLDG 37,RM 2D27,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU GEORG THIEME VERLAG PI STUTTGART PA P O BOX 30 11 20, D-70451 STUTTGART, GERMANY SN 0001-7868 J9 AKTUEL UROL JI Aktuelle Urol. PD MAR PY 1991 VL 22 IS 2 BP 85 EP 91 DI 10.1055/s-2008-1060484 PG 7 WC Urology & Nephrology SC Urology & Nephrology GA FK003 UT WOS:A1991FK00300006 ER PT J AU COHEN, SG DUNN, LM AF COHEN, SG DUNN, LM TI LANDMARK COMMENTARY - LEOPOLD,SIMON AND LEOPOLD,CHARLES ON AIR FILTRATION SO ALLERGY PROCEEDINGS LA English DT Article RP COHEN, SG (reprint author), NIAID,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU OCEAN SIDE PUBLICATIONS INC PI PROVIDENCE PA 95 PITMAN ST, PROVIDENCE, RI 02906 SN 1046-9354 J9 ALLERGY PROC JI Allergy Proc. PD MAR-APR PY 1991 VL 12 IS 2 BP 117 EP 121 DI 10.2500/108854191779011819 PG 5 WC Allergy SC Allergy GA FK469 UT WOS:A1991FK46900010 ER PT J AU KRAUSE, RM AF KRAUSE, RM TI SYPHILIS DURING 1900-1910 - SIMILARITIES TO PRESENT-DAY AIDS (REPRINTED FROM EMORY-UNIVERSITY JOURNAL OF MEDICINE VOL 2, PG 276-280, 1988) SO ALLERGY PROCEEDINGS LA English DT Reprint AB At the beginning of the 20th century, despite 20 years of intensive bacteriologic research, the cause of syphilis was unknown; no diagnostic test and no treatment had been found. Syphilis was one of the leading causes of morbidity and mortality, and those who had the disease were burdened with a social stigma. It was considered a disease of "bad blood." But success was soon to follow. In only 10 years, from 1900 to 1910, the Treponema pallidum was discovered as the cause of syphilis. Animal models were developed for research. The Wassermann test was "invented" for serologic diagnosis, and Paul Ehrlich proved that salvarsan, or 606, was effective for the treatment of syphilis. This success was preceded by 300 failures with related arsenical compounds. The scientific, medical, social, ethical, and economic issues of that day have recurred again with the AIDS epidemic. This earlier drama, therefore, is reflected in the current decade, but the success of Ehrlich, Wassermann, and others in the fight against syphilis is an optimistic omen that researchers will be equally successful in the fight against acquired immune deficiency syndrome (AIDS). RP KRAUSE, RM (reprint author), NIH,FOGARTY INT CTR,BETHESDA,MD 20892, USA. NR 0 TC 5 Z9 5 U1 1 U2 2 PU OCEAN SIDE PUBLICATIONS INC PI PROVIDENCE PA 95 PITMAN ST, PROVIDENCE, RI 02906 SN 1046-9354 J9 ALLERGY PROC JI Allergy Proc. PD MAR-APR PY 1991 VL 12 IS 2 BP 127 EP 132 DI 10.2500/108854191779011756 PG 6 WC Allergy SC Allergy GA FK469 UT WOS:A1991FK46900012 PM 2060782 ER PT J AU BROWN, PM FOSTER, SB AF BROWN, PM FOSTER, SB TI INTEGRATING HEARING AND DEAF STUDENTS ON A COLLEGE CAMPUS - SUCCESSES AND BARRIERS AS PERCEIVED BY HEARING STUDENTS SO AMERICAN ANNALS OF THE DEAF LA English DT Article ID SOCIAL INTEGRATION; IMPAIRED CHILDREN C1 ROCHESTER INST TECHNOL,NAT INST DEAF,OFF POSTSECONDARY CAREER STUDIES & INST RES,ROCHESTER,NY 14623. RP BROWN, PM (reprint author), ROCHESTER INST TECHNOL,NATL INST DEAF,DIV COMMUN PROGRAMS,ROCHESTER,NY 14623, USA. NR 18 TC 13 Z9 13 U1 0 U2 4 PU AMERICAN ANNALS OF THE DEAF PI WASHINGTON PA KDES PAS-6 800 FLORIDA AVE NE, WASHINGTON, DC 20002 SN 0002-726X J9 AM ANN DEAF JI Am. Ann. Deaf PD MAR PY 1991 VL 136 IS 1 BP 21 EP 27 PG 7 WC Education, Special; Rehabilitation SC Education & Educational Research; Rehabilitation GA FE114 UT WOS:A1991FE11400004 PM 2048447 ER PT J AU ADAMS, DR JONES, AM PLOPPER, CG SERABJITSINGH, CJ PHILPOT, RM AF ADAMS, DR JONES, AM PLOPPER, CG SERABJITSINGH, CJ PHILPOT, RM TI DISTRIBUTION OF CYTOCHROME-P-450 MONOXYGENASE ENZYMES IN THE NASAL-MUCOSA OF HAMSTER AND RAT SO AMERICAN JOURNAL OF ANATOMY LA English DT Article ID RESPIRATORY-TRACT; RABBIT NASAL; MONOOXYGENASE; LOCALIZATION; EPITHELIUM; ANTIBODY; LIVER; CELLS; LUNG AB Deposition of inhaled particulates onto the respiratory mucosa is relatively great in that portion of the nasal cavity unprotected by ciliated, goblet, or keratinized superficial cells. The cytochrome P-450 system is an important enzyme system involved in the biotransformation of xenobiotics into metabolites that are more readily absorbed. To examine the transitional region caudal to the nasal vestibule, nasal tissues of hamster and rat were prepared for immunocytochemistry. Blocks of tissue representing four levels along the long axis of the nasal cavity were examined. Paraffin sections were processed through the avidin-biotin peroxidase procedure, with diaminobenzidine tetrahydrochloride as the chromagen. Enzyme localization was accomplished through the use of antibodies for three rabbit cytochrome P-450 isozymes; 2, 5, and 6 (subfamilies IIB, IVB, and IA, respectively); and for rabbit NADPH-cytochrome P-450 reductase. Enzyme distribution was similar in both hamster and rat nasal tissues except in cells of striated and intercalated ducts of nasal glands and in cells of the nasolacrimal duct where immunoreactivity was greater in the hamster. Immunoreactivity for reductase and isozyme 2 was intense in nonciliated cells lining the nonolfactory epithelium, in sustentacular cells of the olfactory epithelium, and in acinar cells of olfactory glands. Distribution of reaction products to isozyme 5 and 6 were similar to but not so intense as those of reductase and isozyme 2. Reaction products for reductase and isozyme 2 occurred generally in the same cellular and intracellular regions with the following exceptions: isozyme 2 was more concentrated in cells of striated ducts and of the nasolacrimal duct, and reductase was more abundant in intercalated ducts of nasal glands. Ciliated and goblet cells in epithelia lining much of the nasal cavity evidenced little reactivity; those ciliated cells adjacent to olfactory mucosa contained reaction product. It is concluded that the nonciliated epithelium adjacent to the internal ostium of the nose contains enzymes essential for biotransforming extrinsic particles that impinge on it from inhaled air currents. C1 UNIV CALIF DAVIS,SCH VET MED,DAVIS,CA 95616. NIEHS,RES TRIANGLE PK,NC 27709. RP ADAMS, DR (reprint author), IOWA STATE UNIV SCI & TECHNOL,COLL VET MED,AMES,IA 50011, USA. NR 35 TC 45 Z9 45 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0002-9106 J9 AM J ANAT PD MAR PY 1991 VL 190 IS 3 BP 291 EP 298 DI 10.1002/aja.1001900309 PG 8 WC Anatomy & Morphology SC Anatomy & Morphology GA EY995 UT WOS:A1991EY99500008 PM 2048556 ER PT J AU RISING, R SWINBURN, B LARSON, K RAVUSSIN, E AF RISING, R SWINBURN, B LARSON, K RAVUSSIN, E TI BODY-COMPOSITION IN PIMA-INDIANS - VALIDATION OF BIOELECTRICAL RESISTANCE SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE BIOELECTRICAL IMPEDANCE; OBESITY; FAT-FREE MASS; FAT MASS ID IMPEDANCE; MASS; OBESITY AB To assess the validity of bioelectrical resistance (BR) in an obese population, body composition was determined by both hydrostatic weighing and by BR in 156 Pima Indian volunteers representing a wide range of body weight (46.1-202.6 kg) and body composition (11-52% fat). A predictive equation was derived by use of data on height, BR, weight, age, and sex from 130 randomly selected volunteers and was applied to the remaining 26 volunteers. When compared with the manufacturer's software, the new equation increased correlations with hydrostatic weighing for predicting percent body fat and fat-free mass (FFM) from 0.70 to 0.92 and 0.79 to 0.97, respectively. The manufacturer's software underestimated FFM by 5.3 +/- 8.6 kg (P < 0.05) when compared with FFM derived from hydrostatic weighing whereas the new equation improved the accuracy to -0.1 +/- 3.3 kg (NS). There were no significant effects of fluid intake (700 mL) or breakfast consumption on body composition as determined by BR. BR represents a simple and accurate way to assess body composition in Pima Indians with our newly derived equation. RP RISING, R (reprint author), NIDDK,CLIN DIABET & NUTR SECT,4212 N 16TH ST,ROOM 541-A,PHOENIX,AZ 85016, USA. NR 16 TC 57 Z9 57 U1 0 U2 0 PU AMER SOC CLIN NUTRITION INC PI BETHESDA PA 9650 ROCKVILLE PIKE SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAR PY 1991 VL 53 IS 3 BP 594 EP 598 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA FA644 UT WOS:A1991FA64400006 PM 2000812 ER PT J AU DIMITROV, NV MEYER, C GILLILAND, D RUPPENTHAL, M CHENOWETH, W MALONE, W AF DIMITROV, NV MEYER, C GILLILAND, D RUPPENTHAL, M CHENOWETH, W MALONE, W TI PLASMA TOCOPHEROL CONCENTRATIONS IN RESPONSE TO SUPPLEMENTAL VITAMIN-E SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE ALPHA-TOCOPHEROL; VITAMIN-E; BIOAVAILABILITY; KINETICS; HUMANS ID ALPHA-TOCOPHEROL; BETA-CAROTENE; CANCER; CELLS; RISK; MEN AB Normal healthy volunteers were studied after various doses of dl-alpha-tocopherol were ingested on different schedules. Administration of 440, 880, or 1320 mg (400, 800, or 1200 IU) of dl-alpha-tocopherol as a single dose resulted in elevation of plasma alpha-tocopherol concentrations that peaked at 12-24 h. Chronic administration of dl-alpha-tocopherol (440, 880, or 1320 mg/d for 28 d) resulted in a steady state that occurred by days 4-5 of supplementation. Discontinuation of the treatment after day 28 was associated with a decline of plasma alpha-tocopherol, which returned to the pretreatment concentrations between 12 and 20 d. The plasma elevation of alpha-tocopherol was affected by dietary fat intake. Individuals consuming a high-fat diet showed significantly greater plasma alpha-tocopherol concentrations as compared with those fed a low-fat diet. The results of this study indicate that plasma kinetics of alpha-tocopherol are similar when supplements of 440, 880, or 1320 mg dl-alpha-tocopherol are given to normal, healthy individuals. C1 MICHIGAN STATE UNIV, DEPT STAT & PROBABIL, E LANSING, MI 48824 USA. MICHIGAN STATE UNIV, DEPT FOOD SCI & HUMAN NUTR, E LANSING, MI 48824 USA. NCI, CANC CONTROL & PREVENT BRANCH, DIV CHEMOPREVENT, BETHESDA, MD 20892 USA. RP MICHIGAN STATE UNIV, COLL HUMAN MED, DEPT MED, B-220 LIFE SCI BLDG, E LANSING, MI 48824 USA. FU NCI NIH HHS [N01-CN-45167] NR 44 TC 108 Z9 112 U1 1 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAR PY 1991 VL 53 IS 3 BP 723 EP 729 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA FA644 UT WOS:A1991FA64400025 PM 2000827 ER PT J AU COSSMAN, J ZEHNBAUER, B GARRETT, CT SMITH, LJ WILLIAMS, M JAFFE, ES HANSON, LO LOVE, J AF COSSMAN, J ZEHNBAUER, B GARRETT, CT SMITH, LJ WILLIAMS, M JAFFE, ES HANSON, LO LOVE, J TI GENE REARRANGEMENTS IN THE DIAGNOSIS OF LYMPHOMA LEUKEMIA - GUIDELINES FOR USE BASED ON A MULTIINSTITUTIONAL STUDY SO AMERICAN JOURNAL OF CLINICAL PATHOLOGY LA English DT Article DE LYMPHOMA; GENES; IMMUNOGLOBULIN; T-CELL RECEPTOR ID CELL NEOPLASIA; T-CELL; TRANSLOCATIONS AB The demonstration of immunoglobulin or T-cell receptor gene rearrangements in human lymphoproliferative processes with the use of DNA hybridization has gained great popularity as a sensitive laboratory adjunct to diagnostic hematopathology. The fact that nearly all B- or T-cell malignant lymphomas and leukemias have one or more rearranged antigen receptor genes provides a biologic basis for a diagnostic test. To formally analyze the sensitivity, specificity, and reproducibility of gene rearrangements in the diagnosis of human lymphoproliferative disease, the authors conducted a large, multiinstitutional study. Through a blinded, controlled approach, gene rearrangement analysis of 275 cases was shown to carry a high correlation with conventional phenotyping and histologic diagnosis, with only minor false-positive and false-negative rates. Significantly, no rearrangements were detected in normal lymphoid tissues or carcinomas, sarcomas, or melanomas. In a randomized study of 50 cases, laboratory results showed a high rate of interlaboratory agreement, regardless of the level of previous experience. Furthermore, the reproducibility of interpretation of data (Southern blot autoradiograms) of 192 cases showed high concordance among 11 observers from multiple laboratories. Based on these findings, the authors propose a set of guidelines for interpretation of gene rearrangement analysis that, if carefully followed, renders this a highly reproducible, safe, and accurate addition to the diagnostic regimen for human lymphoproliferative processes. C1 JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. GEORGE WASHINGTON UNIV,WASHINGTON,DC 20052. UNIV FLORIDA,GAINESVILLE,FL 32611. UNIV VIRGINIA,CHARLOTTESVILLE,VA 22903. NIH,BETHESDA,MD 20892. ONCOR INC,GAITHERSBURG,MD. BIOLINE INC,ROCKVILLE,MD. RP COSSMAN, J (reprint author), GEORGETOWN UNIV,SCH MED,DEPT PATHOL,3900 RESERVOIR RD NW,WASHINGTON,DC 20007, USA. NR 11 TC 91 Z9 91 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0002-9173 J9 AM J CLIN PATHOL JI Am. J. Clin. Pathol. PD MAR PY 1991 VL 95 IS 3 BP 347 EP 354 PG 8 WC Pathology SC Pathology GA EZ514 UT WOS:A1991EZ51400010 PM 1996544 ER PT J AU MEDEIROS, LJ HERRINGTON, RD GONZALEZ, CL JAFFE, ES COSSMAN, J AF MEDEIROS, LJ HERRINGTON, RD GONZALEZ, CL JAFFE, ES COSSMAN, J TI MY4 ANTIBODY STAINING OF NON-HODGKINS-LYMPHOMAS SO AMERICAN JOURNAL OF CLINICAL PATHOLOGY LA English DT Article DE MY4 ANTIBODY; NON-HODGKINS LYMPHOMA; B-CELL PHENOTYPE ID MONOCYTE DIFFERENTIATION ANTIGEN; CHRONIC LYMPHOCYTIC-LEUKEMIA; MYELOMONOCYTIC ANTIGENS; CELL LYMPHOMA; EXPRESSION; CD14; MEMBRANE AB The My4 antibody, one of number of monoclonal antibodies that react with the CD14 antigen, was originally reported to weakly stain monocytes, macrophages, and granulocytes. However, recent studies have shown that the My4 antibody also stains normal peripheral blood B lymphocytes and some subtypes of B-cell non-Hodgkin's lymphoma. Thus, the authors have studied a large series of non-Hodgkin's lymphomas stained with the My4 antibody. In frozen sections of reactive lymph node biopsy specimens, the My4 antibody strongly stained mantle zone B lymphocytes and weakly reacted with dendritic reticulum cells and histiocytes. In a series of 245 non-Hodgkin's lymphomas, the My4 antibody stained 111 (45%) cases: 108 of 189 (57%) B-cell lymphomas, 3 of 50 (6%) T-cell lymphomas, and 0 of 6 null cell lymphomas. My4-positive B-cell lymphomas occurred in all histologic subtypes with the exception of small noncleaved cell lymphomas. Follicular lymphomas were most often My4 positive (82%). My4 antibody staining showed no correlation with Working Formulation grade. All three My4-positive T-cell lymphomas had a mature T-cell phenotype. Seventy-six of the 111 (68%) My4-positive lymphomas were also analyzed with at least one other anti-CD14 antibody, either Mo2 and/or Leu-M3. In all cases the antigens that react with Mo2 and Leu-M3 were not expressed. Thus, the staining of reactive and neoplastic B cells by My4 appears to be unique to this antibody and is not a feature of all anti-CD14 antibodies. RP MEDEIROS, LJ (reprint author), NCI, PATHOL LAB, BLDG 10, ROOM RN108, BETHESDA, MD 20892 USA. NR 18 TC 24 Z9 24 U1 0 U2 0 PU AMER SOC CLINICAL PATHOLOGY PI CHICAGO PA 2100 W HARRISON ST, CHICAGO, IL 60612 USA SN 0002-9173 EI 1943-7722 J9 AM J CLIN PATHOL JI Am. J. Clin. Pathol. PD MAR PY 1991 VL 95 IS 3 BP 363 EP 368 PG 6 WC Pathology SC Pathology GA EZ514 UT WOS:A1991EZ51400012 PM 1705092 ER PT J AU HSING, AW MCLAUGHLIN, JK HRUBEC, Z BLOT, WJ FRAUMENI, JF AF HSING, AW MCLAUGHLIN, JK HRUBEC, Z BLOT, WJ FRAUMENI, JF TI TOBACCO USE AND PROSTATE-CANCER - 26-YEAR FOLLOW-UP OF UNITED-STATES VETERANS SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE PROSTATIC NEOPLASMS; SMOKING; TOBACCO ID CIGARETTE-SMOKING; ALCOHOL-CONSUMPTION; RISK-FACTORS; MEN; MORTALITY; EPIDEMIOLOGY AB A 26-year follow-up of nearly 250,000 US veterans who responded to a questionnaire revealed 4,607 deaths from prostate cancer, providing the largest number of cases to date for evaluating relation to tobacco habits. Cigarette smokers had a significant increase in relative risk (RR = 1.18; 95% confidence interval 1.09-1.28) and a dose response reaching 1.51 among smokers of 40 or more cigarettes per day. Risks were elevated, but not significantly, among users of smokeless tobacco and pipe/cigar smokers. Despite limited data in the literature to support this finding, our study suggests that cigarette smoking may be related to prostate cancer, perhaps through its effect on sex hormone metabolism. RP HSING, AW (reprint author), NCI,DIV CANC ETIOL,EPIDEMIOL & BIOSTAT PROGRAM,EXECUT PLAZA N,ROOM 415,BETHESDA,MD 20892, USA. NR 29 TC 91 Z9 91 U1 2 U2 2 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD MAR 1 PY 1991 VL 133 IS 5 BP 437 EP 441 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FC050 UT WOS:A1991FC05000007 PM 2000853 ER PT J AU HAVERKOS, HW DROTMAN, DP AF HAVERKOS, HW DROTMAN, DP TI KAPOSIS-SARCOMA IN A COHORT OF HOMOSEXUAL AND BISEXUAL MEN - EPIDEMIOLOGY AND ANALYSIS FOR COFACTORS SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Letter ID ACQUIRED IMMUNODEFICIENCY SYNDROME; NITRITE RP HAVERKOS, HW (reprint author), NIDA,ROCKVILLE,MD 20857, USA. NR 9 TC 2 Z9 2 U1 0 U2 0 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD MAR 1 PY 1991 VL 133 IS 5 BP 514 EP 515 PG 2 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FC050 UT WOS:A1991FC05000018 PM 2000863 ER PT J AU SHELTON, MJ MINOR, JR AF SHELTON, MJ MINOR, JR TI AEROSOLIZED PENTAMIDINE IN AIDS PATIENTS WITH ASTHMA SO AMERICAN JOURNAL OF HOSPITAL PHARMACY LA English DT Letter ID PNEUMOCYSTIS-CARINII PNEUMONIA; ACQUIRED IMMUNODEFICIENCY SYNDROME; NEBULIZED PENTAMIDINE; BRONCHOCONSTRICTION; THERAPY C1 NIH,CTR CLIN,DEPT PHARM,BETHESDA,MD 20892. RP SHELTON, MJ (reprint author), SUNY STONY BROOK HOSP,STONY BROOK,NY 11794, USA. NR 16 TC 1 Z9 1 U1 0 U2 0 PU AMER SOC HEALTH-SYSTEM PHARMACISTS PI BETHESDA PA 7272 WISCONSIN AVE, BETHESDA, MD 20814 SN 0002-9289 J9 AM J HOSP PHARM JI Am. J. Hosp. Pharm. PD MAR PY 1991 VL 48 IS 3 BP 556 EP 557 PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EZ524 UT WOS:A1991EZ52400033 PM 2029008 ER PT J AU SHELTON, MJ MINOR, JR AF SHELTON, MJ MINOR, JR TI OCCUPATIONAL EXPOSURE TO AEROSOLIZED PENTAMIDINE ISETHIONATE SO AMERICAN JOURNAL OF HOSPITAL PHARMACY LA English DT Letter C1 NIH,CTR CLIN,DEPT PHARM,BETHESDA,MD 20892. RP SHELTON, MJ (reprint author), SUNY STONY BROOK HOSP,STONY BROOK,NY 11794, USA. NR 14 TC 1 Z9 1 U1 0 U2 0 PU AMER SOC HEALTH-SYSTEM PHARMACISTS PI BETHESDA PA 7272 WISCONSIN AVE, BETHESDA, MD 20814 SN 0002-9289 J9 AM J HOSP PHARM JI Am. J. Hosp. Pharm. PD MAR PY 1991 VL 48 IS 3 BP 557 EP 558 PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EZ524 UT WOS:A1991EZ52400034 PM 2029009 ER PT J AU KARP, BI VIEWEG, WVR AF KARP, BI VIEWEG, WVR TI RAPID CORRECTION OF HYPONATREMIA IN PSYCHIATRIC-PATIENTS WITH POLYDIPSIA SO AMERICAN JOURNAL OF MEDICINE LA English DT Letter ID CENTRAL PONTINE MYELINOLYSIS; ENCEPHALOPATHY C1 UNIV VIRGINIA,WESTERN STATE HOSP,SCH MED,STAUNTON,VA. RP KARP, BI (reprint author), NIH,BETHESDA,MD 20892, USA. NR 14 TC 0 Z9 0 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9343 J9 AM J MED JI Am. J. Med. PD MAR PY 1991 VL 90 IS 3 BP 408 EP 409 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA FC488 UT WOS:A1991FC48800026 PM 2003527 ER PT J AU CAREY, JC BLACKWELDER, WC NUGENT, RP MATTESON, MA RAO, AV ESCHENBACH, DA LEE, MLF RETTIG, PJ REGAN, JA GEROMANOS, KL MARTIN, DH PASTOREK, JG GIBBS, RS LIPSCOMB, KA AF CAREY, JC BLACKWELDER, WC NUGENT, RP MATTESON, MA RAO, AV ESCHENBACH, DA LEE, MLF RETTIG, PJ REGAN, JA GEROMANOS, KL MARTIN, DH PASTOREK, JG GIBBS, RS LIPSCOMB, KA TI ANTEPARTUM CULTURES FOR UREAPLASMA-UREALYTICUM ARE NOT USEFUL IN PREDICTING PREGNANCY OUTCOME SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Article DE UREAPLASMA-UREALYTICUM; LOW BIRTH WEIGHT; PRETERM LABOR; PRETERM BIRTH; PRETERM PREMATURE RUPTURE OF MEMBRANES ID CHLAMYDIA-TRACHOMATIS INFECTIONS; GENITAL MYCOPLASMAS; BIRTH-WEIGHT; CHORIOAMNIONITIS; COLONIZATION; PREMATURITY; ASSOCIATION; MORTALITY; PLACENTA; HOMINIS AB To test the hypothesis that genital colonization with Ureaplasma urealyticum would predict adverse pregnancy outcome, 4934 women from five medical centers were evaluated for vaginal colonization with U. urealyticum between 23 and 26 weeks' gestation and followed up to delivery. U. urealyticum colonization was associated with maternal age, parity, racial-ethnic group, martial status, income, education, smoking, number of sexual partners, and colonization with Trichomonas vaginalis, Mycoplasma hominis, and bacterial vaginosis. After adjustment for medical and sociodemographic factors in a multivariate analysis, there was no difference in the mean birth weight or proportion of low-birth-weight infants delivered by women who carried U. urealyticum and those who did not. U. urealyticum colonization at 23 to 26 weeks was not associated with preterm rupture of membranes, preterm labor, or preterm delivery. A positive vaginal culture for U. urealyticum in midgestation does not predict those women at risk for preterm labor, preterm delivery, preterm premature rupture of membranes, or delivery of a low-birth-weight infant. C1 NICHHD, BETHESDA, MD 20892 USA. NIAID, BETHESDA, MD 20892 USA. RES TRIANGLE INST, RES TRIANGLE PK, NC 27709 USA. UNIV TEXAS SAN ANTONIO, SAN ANTONIO, TX 78285 USA. UNIV OKLAHOMA, HLTH SCI CTR, OKLAHOMA CITY, OK 73190 USA. LOUISIANA STATE UNIV, BATON ROUGE, LA 70803 USA. UNIV WASHINGTON, SEATTLE, WA 98195 USA. COLUMBIA UNIV, NEW YORK, NY 10027 USA. FU NICHD NIH HHS [HD-3-2832, HD-3-2833, HD-3-2834] NR 20 TC 88 Z9 91 U1 0 U2 3 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9378 EI 1097-6868 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD MAR PY 1991 VL 164 IS 3 BP 728 EP 733 PG 6 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA FC256 UT WOS:A1991FC25600004 PM 2003532 ER PT J AU ESCHENBACH, DA NUGENT, RP RAO, AV COTCH, MF GIBBS, RS LIPSCOMB, KA MARTIN, DH PASTOREK, JG RETTIG, PJ CAREY, JC REGAN, JA GEROMANOS, KL LEE, MLF POOLE, WK EDELMAN, R AF ESCHENBACH, DA NUGENT, RP RAO, AV COTCH, MF GIBBS, RS LIPSCOMB, KA MARTIN, DH PASTOREK, JG RETTIG, PJ CAREY, JC REGAN, JA GEROMANOS, KL LEE, MLF POOLE, WK EDELMAN, R TI A RANDOMIZED PLACEBO-CONTROLLED TRIAL OF ERYTHROMYCIN FOR THE TREATMENT OF UREAPLASMA-UREALYTICUM TO PREVENT PREMATURE DELIVERY SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Article DE UREAPLASMA-UREALYTICUM; ERYTHROMYCIN; LOW BIRTH WEIGHT; PRETERM BIRTH; PRETERM PREMATURE RUPTURE OF MEMBRANES; CLINICAL TRIAL ID GENITAL MYCOPLASMAS; CHLAMYDIA-TRACHOMATIS; BACTERIAL VAGINOSIS; BIRTH-WEIGHT; PREGNANCY; INFECTION; CHORIOAMNIONITIS; COLONIZATION AB Ureaplasma urealyticum has been associated with low birth weight and histologic chorioamnionitis and it is a frequent isolate from the chorioamnion of patients who are delivered prematurely. In prior clinical trials using antibiotics active against U. urealyticum, antibiotic treatment was associated with reduced prematurity and increased mean birth weight. In this multicenter, randomized, double-blind clinical trial, pregnant women with U. urealyticum were treated with 333 mg of erythromycin base or placebo three times daily, starting between 26 and 30 weeks' gestation and continuing through 35 completed weeks of pregnancy. Women with urinary tract infection or Neisseria gonorrhoeae infection were excluded from the trial, and women with Chlamydia trachomatis or group B streptococci were excluded from these analyses. Erythromycin did not eliminate U. urealyticum from the lower genital tract. There were no significant differences between erythromycin- and placebo-treated women in infant birth weight or gestational age at delivery, in frequency of premature rupture of membranes, or in neonatal outcome. C1 UNIV WASHINGTON,SEATTLE,WA 98195. NIAID,BETHESDA,MD 20892. RES TRIANGLE INST,RES TRIANGLE PK,NC 27709. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. LOUISIANA STATE UNIV,BATON ROUGE,LA 70803. UNIV OKLAHOMA,HLTH SCI CTR,OKLAHOMA CITY,OK 73190. COLUMBIA UNIV,NEW YORK,NY 10027. OI Cotch, Mary Frances/0000-0002-2046-4350 FU Intramural NIH HHS [Z99 EY999999]; NICHD NIH HHS [HD-3-2834, HD-3-2833, HD-3-2832] NR 20 TC 103 Z9 106 U1 0 U2 4 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-9378 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD MAR PY 1991 VL 164 IS 3 BP 734 EP 742 PG 9 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA FC256 UT WOS:A1991FC25600005 PM 2003533 ER PT J AU GRIGIONI, WF GARBISA, S DERRICO, A BACCARINI, P STETLERSTEVENSON, WG LIOTTA, LA MANCINI, AM AF GRIGIONI, WF GARBISA, S DERRICO, A BACCARINI, P STETLERSTEVENSON, WG LIOTTA, LA MANCINI, AM TI EVALUATION OF HEPATOCELLULAR-CARCINOMA AGGRESSIVENESS BY A PANEL OF EXTRACELLULAR-MATRIX ANTIGENS SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Article ID BASEMENT-MEMBRANE COMPONENTS; CELL-PROLIFERATION; TUMOR INVASION; COLLAGEN; LIVER; LAMININ AB Invasion and metastasis requires a series of interactions between malignant cells and the extracellular matrix (ECM). Antigen markers that relate to these interactions were evaluated for prognostic correlation in human hepatocellular carcinoma. Basement membrane type IV collagen (cIV), type IV collagenase (cIVase), laminin, and laminin receptors (LRs) - all ECM antigens previously proposed to be modulated in association with tumor aggressiveness-were immunohistochemically investigated in 30 cases of hepatocellular carcinomas (HCCs). The pattern of antigen expression was correlated with 1) 36 months' clinical follow-up and 2) the pathologic grade. As a means of estimating the proliferation fraction, an additional antigen, Ki67, was also studied in this series. There were major differences in the distribution of cIV and laminin, and in the quantity of cIVase-, LR-, and Ki67-positive cells associated with grade and prognosis. A smaller quantity of cIV and laminin and a higher number of cIVase-, LR-, and Ki67-positive cells were detected in the poorly differentiated compared with the well-differentiated HCCs. The tumors with lower immunoreactivity for cIV and laminin components accompanied by a higher number of cIVase-, LR-, and Ki67-positive cells fall into a group with the poorest overall survival (P < 0.006). The panel of antigens is proposed as a useful prognostic tool for evaluating HCC tumor aggressiveness. C1 UNIV BOLOGNA,INST HISTOL,I-40138 BOLOGNA,ITALY. UNIV BOLOGNA,CTR CANC RES,I-40138 BOLOGNA,ITALY. NIH,PATHOL LAB,BETHESDA,MD 20892. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 32 TC 102 Z9 107 U1 2 U2 3 PU AMER SOC INVESTIGATIVE PATHOLOGY, INC PI BALTIMORE PA 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD MAR PY 1991 VL 138 IS 3 BP 647 EP 654 PG 8 WC Pathology SC Pathology GA FA838 UT WOS:A1991FA83800015 PM 1848041 ER PT J AU KAKU, T LAKATTA, E FILBURN, C AF KAKU, T LAKATTA, E FILBURN, C TI ALPHA-ADRENERGIC REGULATION OF PHOSPHOINOSITIDE METABOLISM AND PROTEIN-KINASE-C IN ISOLATED CARDIAC MYOCYTES SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE CARDIAC CONTRACTILITY; MYOCARDIAL LIPID METABOLISM; INOSITOL TRISPHOSPHATE; PROTEIN KINASE-C TRANSLOCATION ID INOSITOL TRISPHOSPHATE; RAT-HEART; SARCOPLASMIC-RETICULUM; ADRENOCEPTOR STIMULATION; CALCIUM RELEASE; SKELETAL-MUSCLE; PHOSPHATIDYLINOSITOL METABOLISM; ALPHA-1-ADRENERGIC STIMULATION; MYOFIBRILLAR RESPONSIVENESS; VENTRICULAR MYOCYTES AB alpha-1-Adrenergic regulation of phosphoinositide metabolism and protein kinase C translocation was studied in isolated rat cardiac myocytes. Exposure of [H-3]inositol-labeled myocytes to norepinephrine in the presence of propranolol caused a dose-dependent increase in [H-3]inositol phosphates. Norepinephrine also increased the level of membrane-associated protein kinase C from approximately 10% of total activity to 18%, with a dose response similar to that for generation of inositol phosphates. Depolarization of myocytes with 30 mM KCl had no effect on inositol phosphates or membrane-associated protein kinase C but potentiated the effect of submaximal norepinephrine on both parameters. The potentiation of protein kinase C translocation was amplified when extracellular Ca2+ was increased to 4 mM, resulting in membrane association of one-third of the total cellular activity. These data show that activation of protein kinase C occurs during alpha-1-adrenergic stimulation of cardiac myocytes and that elevation of intracellular Ca2+ amplifies this effect at least in part through increased phosphoinositide metabolism. C1 NIA,GERONTOL RES CTR,CARDIOVASC SCI LAB,4940 EASTERN AVE,BALTIMORE,MD 21224. NIA,GERONTOL RES CTR,BIOL CHEM LAB,BALTIMORE,MD 21224. NR 57 TC 74 Z9 74 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD MAR PY 1991 VL 260 IS 3 BP C635 EP C642 PN 1 PG 8 WC Physiology SC Physiology GA FC250 UT WOS:A1991FC25000029 PM 1848404 ER PT J AU RAZ, I KATZ, A SPENCER, MK AF RAZ, I KATZ, A SPENCER, MK TI EPINEPHRINE INHIBITS INSULIN-MEDIATED GLYCOGENESIS BUT ENHANCES GLYCOLYSIS IN HUMAN SKELETAL-MUSCLE SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE HEXOSE MONOPHOSPHATES; GLUCOSE 1,6-BISPHOSPHATE; FRUCTOSE 2,6-BISPHOSPHATE; FRUCTOSE 1,6-BISPHOSPHATE; LACTATE; ADENOSINE 5'-TRIPHOSPHATE; PHOSPHOCREATINE; GLYCOGEN SYNTHASE; PHOSPHORYLASE; URIDINE 5'-DIPHOSPHATE-GLUCOSE ID EUGLYCEMIC HYPERINSULINEMIA; FRUCTOSE 2,6-BISPHOSPHATE; GLUCOSE 1,6-BISPHOSPHATE; METABOLISM; RAT; SENSITIVITY; INVIVO; ASSAY AB The effect of epinephrine (E) infusion on insulin-mediated glucose metabolism in humans has been studied. Eight glucose-tolerant men were studied on two separate occasions: 1) during 120 min of euglycemic hyperinsulinemia (UH, approximately 5 mM; 40 mU.m-2.min-1); and 2) during UH while E was infused (UHE, 0.05-mu-g.kg-1.min-1). Biopsies were taken from the quadriceps femoris muscle before and after each clamp. Glucose disposal, correcting for endogenous glucose production, was 36 +/- 3 and 18 +/- 2 (SE) mu-mol.kg fat-free mass (FFM)-1.min-1 during the last 40 min of UH and UHE, respectively (P < 0.001). Nonoxidative glucose disposal (presumably glycogenesis) averaged 23.0 +/- 3.0 and 4.0 +/- 1.1 (P < 0.001), whereas carbohydrate oxidation (which is proportional to glycolysis) averaged 13.1 +/- 1.4 and 15.3 +/- 1.1-mu-mol.kg FFM-1.min-1 (P < 0.05) during UH and UHE, respectively. UHE resulted in significantly higher contents of UDP-glucose, hexose monophosphates, postphosphofructokinase intermediates, and glucose 1,6-bisphosphate (G-1,6-P2) in muscle (P < 0.05-0.001), but there were no significant differences in high-energy phosphates or fructose 2,6-bisphosphate (F-2,6-P2) between treatments. Fractional activities of phosphorylase increased (P < 0.01), and glycogen synthase decreased (P < 0.001) during UHE. It is concluded that E inhibits insulin-mediated glycogenesis because of an inactivation of glycogen synthase and an activation of glycogenolysis. E also appears to inhibit insulin-mediated glucose utilization, at least partly, because of an increase in G-6-phosphate (which inhibits hexokinase) and enhances glycolysis by G-1,6-P2-, fructose 6-phosphate-, and F-1,6-P2-mediated activation of PFK. C1 UNIV ILLINOIS,DEPT KINESIOL,906 S GOODWIN AVE,URBANA,IL 61801. NIDDKD,CLIN DIABET & NUTR SECT,PHOENIX,AZ 85016. NR 33 TC 60 Z9 60 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD MAR PY 1991 VL 260 IS 3 BP E430 EP E435 PN 1 PG 6 WC Physiology SC Physiology GA FC250 UT WOS:A1991FC25000048 PM 1900669 ER PT J AU SPENCER, MK KATZ, A RAZ, I AF SPENCER, MK KATZ, A RAZ, I TI EPINEPHRINE INCREASES TRICARBOXYLIC-ACID CYCLE INTERMEDIATES IN HUMAN SKELETAL-MUSCLE SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE CITRATE; MALATE; FUMARATE; ALANINE; ASPARTATE; GLUTAMATE; PYRUVATE; ADENINE NUCLEOTIDES; INOSINE MONOPHOSPHATE; OXYGEN UPTAKE; EUGLYCEMIC HYPERINSULINEMIA ID PURINE-NUCLEOTIDE CYCLE; AMMONIA PRODUCTION; NADH; EXERCISE; REST AB The effects of epinephrine (E) and insulin infusions on the contents of tricarboxylic acid cycle intermediates (TCAI), adenine nucleotides and their catabolites, and amino acids in skeletal muscle have been investigated. Eight men were studied on two separate occasions: 1) during 120 min of euglycemic hyperinsulinemia (UH, approximately 5 mM; 40 mU.m-2.min-1) and 2) during UH while E was infused (UHE, 0.05 mu-g.kg-1.min-1). Biopsies were taken from the quadriceps femoris muscle before and after each clamp. The sum of citrate, malate, and fumarate in muscle did not change significantly during UH (P > 0.05) but doubled during UHE (P < 0.001). There were no significant changes in any of the adenine nucleotides, their catabolites (including inosine monophosphate), or aspartate during UH and UHE (P > 0.05); nor were there any significant changes in pyruvate or alanine contents during UH (P > 0.05). On the other hand, there were significant increases in pyruvate and alanine contents during UHE (P < 0.01 and 0.05, respectively), suggesting that there was increased production of 2-oxoglutarate (a TCAI) via the alanine aminotransferase (ALT) reaction. It is concluded that E infusion increases the contents of TCAI in human skeletal muscle, and it is likely that at least part of the increase is attributable to increased flux through the ALT reaction. C1 UNIV ILLINOIS,DEPT KINESIOL,906 S GOODWIN AVE,URBANA,IL 61801. NIDDKD,CLIN DIABET & NUTR SECT,PHOENIX,AZ 85016. NR 27 TC 17 Z9 17 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD MAR PY 1991 VL 260 IS 3 BP E436 EP E439 PN 1 PG 4 WC Physiology SC Physiology GA FC250 UT WOS:A1991FC25000049 PM 2003596 ER PT J AU DARKO, DF WILSON, NW GILLIN, JC GOLSHAN, S AF DARKO, DF WILSON, NW GILLIN, JC GOLSHAN, S TI A CRITICAL-APPRAISAL OF MITOGEN-INDUCED LYMPHOCYTE-PROLIFERATION IN DEPRESSED-PATIENTS SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID AFFECTIVE-DISORDERS; IMMUNITY; SCHIZOPHRENIA; SCHEDULE; ILLNESS AB Objective: The authors' goal was to evaluate the utility of mitogen-induced lymphocyte proliferation assays in clinical research in psychoimmunology. Method: They examined 23 depressed patients and 23 matched comparison subjects with this assay. There were no significant differences between these groups. They then combined the results of this study with the results of their previous study of 20 depressed patients and 20 comparison subjects to examine possible determinants of lymphocyte proliferation in depression. Results: Depressed patients with lower proliferative responses than their matched comparison subjects had lower depression subscale, anergia subscale, and total scores on the Brief Psychiatric Rating Scale than did patients with higher proliferative responses than their matched comparison subjects. This finding was unexpected and unexplained. Depressed patients with lower proliferative responses than their matched comparison subjects also had fewer obsessions and compulsions and less psychomotor agitation according to the Schedule for Affective Disorders and Schizophrenia interview than did patients with higher proliferative responses than their matched comparison subjects. Stepwise discriminant analysis and cluster analysis contributed little further understanding of the determinants of in vitro lymphocyte proliferation of cells from depressed patients. Conclusions: Longitudinal studies using multiple serial determinations of mitogen-induced lymphocyte proliferation are the minimal design needed to make this assay useful in further evaluating any immune system changes in depression. C1 UNIV CALIF SAN DIEGO,SCH MED,DEPT PSYCHIAT,NIMH,CLIN RES CTR,LA JOLLA,CA 92093. UNIV CALIF SAN DIEGO,SCH MED,DIV IMMUNOL & ALLERGY,DEPT PEDIAT LABS,LA JOLLA,CA 92093. RP DARKO, DF (reprint author), VET AFFAIRS MED CTR,116A,3350 LA JOLLA VILLAGE DR,SAN DIEGO,CA 92161, USA. FU NIMH NIH HHS [MH-30914, MH-42762] NR 22 TC 26 Z9 26 U1 1 U2 1 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD MAR PY 1991 VL 148 IS 3 BP 337 EP 344 PG 8 WC Psychiatry SC Psychiatry GA EZ736 UT WOS:A1991EZ73600007 PM 1992836 ER PT J AU KIRSCHSTEIN, RL AF KIRSCHSTEIN, RL TI RESEARCH ON WOMENS HEALTH SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article RP KIRSCHSTEIN, RL (reprint author), NIH,RES WOMENS HLTH,BLDG 31,ROOM 4A52,900 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 3 TC 26 Z9 26 U1 0 U2 0 PU AMER PUBLIC HEALTH ASSN INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD MAR PY 1991 VL 81 IS 3 BP 291 EP 293 DI 10.2105/AJPH.81.3.291 PG 3 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FF473 UT WOS:A1991FF47300003 PM 1994737 ER PT J AU ERSHOW, AG BROWN, LM CANTOR, KP AF ERSHOW, AG BROWN, LM CANTOR, KP TI INTAKE OF TAPWATER AND TOTAL WATER BY PREGNANT AND LACTATING WOMEN SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article AB Background. Despite theoretically higher requirements for water due to physiologic demands of pregnancy and lactation, little is known of actual ranges of intake in pregnant and lactating women. Methods. Population-based estimates of total water and tapwater intake in women of reproductive age were derived using data from the 1977-78 USDA Nationwide Food Consumption Survey. Three-day average intakes were calculated for 188 pregnant women, 77 lactating women, and 6,201 non-pregnant, non-lactating control women. Results. Total water intake (mean +/- SD) was 1,940 +/- 686 g/day (median 1,835) for control women, 2,076 +/- 743 g/day (median 1,928) for pregnant women and 2,242 +/- 658 g/day (median 2,164) for lactating women. Tapwater intake was 1,157 +/- 635 g/day (median 1,065) for control women, 1,189 +/- 699 g/day (median 1,063) for pregnant women, and 1,310 +/- 591 g/day (median 1,330) for lactating women. Total water intake was equal to or greater than 3,000 g/day among 7 percent of control women, 11 percent of pregnant women, and 13 percent of lactating women. Tapwater intake was equal to or greater than 2,000 g/day among 10 percent of control women, 15 percent of pregnant women, and 8 percent of lactating women. Conclusions. These results should be useful in estimating amounts of nutrients and toxic substances that women of reproductive age obtain through the water supply. C1 NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. NCI,BIOSTAT BRANCH,BETHESDA,MD 20892. RP ERSHOW, AG (reprint author), NHLBI,LIPID METAB ATHEROGENESIS BRANCH,BETHESDA,MD 20892, USA. NR 23 TC 52 Z9 55 U1 0 U2 1 PU AMER PUBLIC HEALTH ASSN INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD MAR PY 1991 VL 81 IS 3 BP 328 EP 334 DI 10.2105/AJPH.81.3.328 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FF473 UT WOS:A1991FF47300009 PM 1994741 ER PT J AU JONES, CJ SCHIFFMAN, MH KURMAN, R JACOB, P BENOWITZ, NL AF JONES, CJ SCHIFFMAN, MH KURMAN, R JACOB, P BENOWITZ, NL TI ELEVATED NICOTINE LEVELS IN CERVICAL LAVAGES FROM PASSIVE SMOKERS SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Note ID CIGARETTE-SMOKING; NEOPLASIA; COTININE; CONSTITUENTS; NONSMOKERS; SERUM; MUCUS AB One hundred forty-five non-smokers found to have normal cytologic diagnoses on routine Pap smears were interviewed regarding environmental exposure to tobacco smoke, and a 3 ml saline lavage of the cervix was collected for measurement of cervical nicotine levels by gas chromatography-mass spectroscopy. Nicotine levels tended to be highest among women exposed to tobacco smoke in the home, intermediate in women exposed only outside the home, and lowest in women recalling no exposure (p = 0.001). C1 NCI,BIOSTAT BRANCH,EXECUT PLAZA N 443,BETHESDA,MD 20892. GEORGETOWN UNIV HOSP,WASHINGTON,DC 20007. UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. FU NCI NIH HHS [CA 32389]; NIDA NIH HHS [DA 02277] NR 8 TC 24 Z9 28 U1 0 U2 0 PU AMER PUBLIC HEALTH ASSN INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD MAR PY 1991 VL 81 IS 3 BP 378 EP 379 DI 10.2105/AJPH.81.3.378 PG 2 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FF473 UT WOS:A1991FF47300016 PM 1994747 ER PT J AU BARANIUK, JN LUNDGREN, JD OKAYAMA, M GOFF, J MULLOL, J MERIDA, M SHELHAMER, JH KALINER, MA AF BARANIUK, JN LUNDGREN, JD OKAYAMA, M GOFF, J MULLOL, J MERIDA, M SHELHAMER, JH KALINER, MA TI SUBSTANCE-P AND NEUROKININ-A IN HUMAN NASAL-MUCOSA SO AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY LA English DT Article ID GENE-RELATED PEPTIDE; INCREASED VASCULAR-PERMEABILITY; HUMAN LOWER AIRWAYS; MULTIPLE TACHYKININS; SENSORY NERVES; NEUROPEPTIDE-K; SPINAL-CORD; CAPSAICIN; STIMULATION; RELEASE AB The tachykinins substance P (SP) and neurokinin A (NKA) were studied in human inferior turbinate nasal mucosa by radioimmunoassay, immunohistochemistry, and autoradiography and for their effect upon mucus release in an in vitro culture system in order to infer their potential functions in the upper respiratory tract. Similar amounts of SP (1.03 +/- 0.12 pmol/g wet weight; mean +/- SEM; n = 26) and NKA (0.76 +/- 0.23; n = 7) were found. NKA and SP immunoreactive nerve fibers were found in the walls of arterioles, venules, and sinusoids and as individual fibers in gland acini, near the basement membrane, and in the epithelium. [I-125]SP bound to arterioles, venules, and glands. [I-125]NKA bound only to arterioles. In short-term explant culture of fragments of human nasal mucosa, both 1-mu-M SP and 1-mu-M NKA stimulated release of [H-3]glucosamine-labeled respiratory glycoconjugates. These results indicate that SP and NKA have similar distributions in nociceptive sensory nerves in human nasal mucosa. The distribution of [I-125]SP binding sites is consistent with a role for SP as a vasodilator and mucous secretagogue. The presence of [I-125] NKA binding sites on vessels suggests a primary role for NKA in regulating vasomotor tone. C1 NIAID, CLIN INVEST LAB, ALLERG DIS SECT, BETHESDA, MD 20892 USA. NIH, CTR CLIN, DEPT MED, DIV CRIT CARE, BETHESDA, MD 20892 USA. GEORGETOWN UNIV, DIV VIROL, WASHINGTON, DC 20057 USA. GEORGETOWN UNIV HOSP, DEPT OTOLARYNGOL, WASHINGTON, DC 20007 USA. GEORGETOWN UNIV HOSP, DEPT PEDIAT, WASHINGTON, DC 20007 USA. OI Lundgren, Jens/0000-0001-8901-7850 NR 46 TC 141 Z9 142 U1 0 U2 2 PU AMER THORACIC SOC PI NEW YORK PA 25 BROADWAY, 18 FL, NEW YORK, NY 10004 USA SN 1044-1549 EI 1535-4989 J9 AM J RESP CELL MOL JI Am. J. Respir. Cell Mol. Biol. PD MAR PY 1991 VL 4 IS 3 BP 228 EP 236 PG 9 WC Biochemistry & Molecular Biology; Cell Biology; Respiratory System SC Biochemistry & Molecular Biology; Cell Biology; Respiratory System GA FA840 UT WOS:A1991FA84000006 PM 1705809 ER PT J AU MILLER, DL AF MILLER, DL TI RADIOLOGY OF THE CERVICAL-SPINE IN TRAUMA PATIENTS SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Letter RP MILLER, DL (reprint author), NIH,WARREN GRANT MAGNUSON CLIN CTR,BETHESDA,MD 20892, USA. NR 2 TC 1 Z9 1 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD MAR PY 1991 VL 156 IS 3 BP 638 EP 638 PG 1 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EY478 UT WOS:A1991EY47800049 PM 1899772 ER PT J AU ISSAQ, HJ JAENCHEN, DE AF ISSAQ, HJ JAENCHEN, DE TI HIGH-PERFORMANCE TLC IN THE CLINICAL LABORATORY SO AMERICAN LABORATORY LA English DT Article ID THIN-LAYER CHROMATOGRAPHY AB The performance characteristics of a new high-performance gel permeation chromatography system are described. This modestly priced system includes a precision pump, high efficiency rugged columns, a stable detector, and a versatile data system. C1 CAMAG,MUTTENZ,SWITZERLAND. RP ISSAQ, HJ (reprint author), NCI,FREDERICK CANC RES FACIL,PROGRAM RES INC,FREDERICK,MD 21701, USA. NR 19 TC 3 Z9 3 U1 0 U2 0 PU INT SCIENTIFIC COMMUN INC PI SHELTON PA PO BOX 870, 30 CONTROLS DRIVE, SHELTON, CT 06484-0870 SN 0044-7749 J9 AM LAB JI Am. Lab. PD MAR PY 1991 VL 23 IS 5 BP D44 EP & PG 0 WC Chemistry, Analytical; Instruments & Instrumentation SC Chemistry; Instruments & Instrumentation GA FA663 UT WOS:A1991FA66300006 ER PT J AU HUTNER, M WINDLE, C AF HUTNER, M WINDLE, C TI NIMH SUPPORT OF RURAL MENTAL-HEALTH SO AMERICAN PSYCHOLOGIST LA English DT Article RP HUTNER, M (reprint author), NIMH,OPAC,ROOM 17C-20,5600 FISHERS LANE,ROCKVILLE,MD 20852, USA. NR 15 TC 6 Z9 6 U1 0 U2 0 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0003-066X J9 AM PSYCHOL JI Am. Psychol. PD MAR PY 1991 VL 46 IS 3 BP 240 EP 243 DI 10.1037//0003-066X.46.3.240 PG 4 WC Psychology, Multidisciplinary SC Psychology GA FA439 UT WOS:A1991FA43900006 PM 2035934 ER PT J AU PEDEN, DB BROWN, ME WADE, Y RAPHAEL, GD BERKEBILE, C KALINER, MA AF PEDEN, DB BROWN, ME WADE, Y RAPHAEL, GD BERKEBILE, C KALINER, MA TI HUMAN NASAL GLANDULAR SECRETION OF NOVEL ANTIOXIDANT ACTIVITY - CHOLINERGIC CONTROL SO AMERICAN REVIEW OF RESPIRATORY DISEASE LA English DT Article ID EPITHELIAL LINING FLUID; RESPIRATORY-DISTRESS SYNDROME; PULMONARY OXYGEN-TOXICITY; PATHO-PHYSIOLOGY; OXIDANT ACTIVITY; RHINITIS; PROTEIN; GLUTATHIONE; DEFICIENCY; SUPEROXIDE AB Exposure of the respiratory mucosa to oxygen-enriched air contributes to the generation of the lung damage in both adult respiratory distress syndrome and bronchopulmonary dysplasia. Recent work has identified the nasal submucosal gland as the source of diverse molecules important in mucous membrane host defense. We searched for the presence of antioxidant activity in nasal glandular secretions, the absence of which could possibly predispose to oxygen-induced injury. Employing a low molecular weight preparation of nasal secretions (a pooled concentrate passed over a 10,000-dalton molecular sieve), antioxidant activity capable of inhibiting both horseradish peroxidase and Fenton reagent reactions was discovered. The following lines of evidence suggest that submucosal glands are the source of this activity. (1) Antioxidant activity present in resting, baseline nasal washings is significantly increased after cholinergic stimulation either in response to topical methacholine or induced by a gustatory reflex. (2) Application of atropine reduced the antioxidant activity to baseline levels after either of the cholinergic stimuli. (3) Levels of antioxidant activity correlated very closely with the secretion of lactoferrin, a recognized product secreted solely from the serous cell of the submucosal gland. The antioxidant activity is due to novel, previously unrecognized molecules. This activity is found in nasal secretions containing molecules < 10,000 daltons, is unaffected by N-ethyl maleimide (which inactivates glutathione, another low molecular weight antioxidant), is not associated with the capacity to reduce cytochrome c (as seen with ascorbic acid), and resides in the water soluble pool of secretions (in contrast to vitamin E, another putative antioxidant). Thus, cholinergically stimulated nasal secretions possess novel low molecular weight antioxidant activity that might be important in protecting the mucosa against oxidant injury. RP PEDEN, DB (reprint author), NIAID,CLIN INVEST LAB,ALLERG DIS SECT,BLDG 10,ROOM 11C-209,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 44 TC 7 Z9 7 U1 0 U2 2 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 0003-0805 J9 AM REV RESPIR DIS JI Am. Rev. Respir. Dis. PD MAR PY 1991 VL 143 IS 3 BP 545 EP 552 PG 8 WC Respiratory System SC Respiratory System GA FA931 UT WOS:A1991FA93100017 PM 2001065 ER PT J AU TAMURA, N MOLLER, DR BALBI, B CRYSTAL, RG AF TAMURA, N MOLLER, DR BALBI, B CRYSTAL, RG TI PREFERENTIAL USAGE OF THE T-CELL ANTIGEN RECEPTOR BETA-CHAIN CONSTANT REGION C-BETA-1 ELEMENT BY LUNG LYMPHOCYTES-T OF PATIENTS WITH PULMONARY SARCOIDOSIS SO AMERICAN REVIEW OF RESPIRATORY DISEASE LA English DT Article ID LOWER RESPIRATORY-TRACT; VARIABLE REGION; INTERLEUKIN-2 RECEPTOR; CHRONIC INFLAMMATION; SPONTANEOUS RELEASE; DISEASE-ACTIVITY; UNKNOWN CAUSE; ALPHA-CHAIN; GENES; EXPRESSION AB Evaluation of the T-cells accumulating at sites of disease in active sarcoidosis suggests the accumulation process is not random, evidenced by a bias in the types of T-cells present. To evaluate the concept that this bias extends to the accumulation of T-cells with the preferential use of specific T-cell antigen receptor (TCR) beta-chain constant region elements, beta-chain mRNA transcripts of lung and blood T-cells of normal subjects and patients with pulmonary sarcoldosis were compared for the relative usage of constant region beta-1 or beta-2 elements. Quantitative evaluation of C-beta-1 and C-beta-2 mRNA transcripts demonstrated a C-beta/C-beta-2 usage in normal blood of 0.63 +/- 0.02, similar to that of normal lung (0.64 +/- 0.06, p > 0.7), and in sarcoid blood (0.59 +/- 0.03, p > 0.2). In contrast, the lung T-lymphocytes of patients with sarcoldosis reflected a marked bias in the usage of C-beta-1 elements (C-beta-1/C-beta-2: 0.88 +/- 0.06, p < 0.001 compared with sarcoid blood and normal blood; p < 0.02 compared with normal lung). Interestingly, a subgroup of these patients (six of 18) showed a markedly exaggerated skewing in the use of C-beta-1 elements (C-beta-1/C-beta-2 ratio > 1, i.e., > 3 standard deviations above mean), demonstrating heterogeneity among sarcoid patients with regard to specific C-beta-1 usage. These observations provide further evidence that there is a bias in the type of T-lymphocytes that accumulate in affected organs in sarcoidosis, and they are consistent with the concept that there is selection for T-lymphocytes with specific T-cell antigen receptors in association with sarcoid inflammation. C1 NHLBI,PULM BRANCH,BLDG 10,ROOM 6D03,BETHESDA,MD 20892. NR 35 TC 26 Z9 26 U1 0 U2 1 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 0003-0805 J9 AM REV RESPIR DIS JI Am. Rev. Respir. Dis. PD MAR PY 1991 VL 143 IS 3 BP 635 EP 639 PG 5 WC Respiratory System SC Respiratory System GA FA931 UT WOS:A1991FA93100031 PM 1848052 ER PT J AU CHERNIACK, RM CRYSTAL, RG KALICA, AR AF CHERNIACK, RM CRYSTAL, RG KALICA, AR TI CURRENT CONCEPTS IN IDIOPATHIC PULMONARY FIBROSIS - A ROAD MAP FOR THE FUTURE SO AMERICAN REVIEW OF RESPIRATORY DISEASE LA English DT Editorial Material C1 NHLBI,INTERSTITIAL LUNG DIS BRANCH,WESTWOOD BLDG,ROOM 6A09,BETHESDA,MD 20892. NR 0 TC 59 Z9 59 U1 0 U2 1 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 0003-0805 J9 AM REV RESPIR DIS JI Am. Rev. Respir. Dis. PD MAR PY 1991 VL 143 IS 3 BP 680 EP 683 PG 4 WC Respiratory System SC Respiratory System GA FA931 UT WOS:A1991FA93100038 PM 2001083 ER PT J AU KAYASTHA, AM MILES, EW AF KAYASTHA, AM MILES, EW TI A COLORIMETRIC ASSAY FOR A PYRIDOXAL PHOSPHATE-DEPENDENT BETA-REPLACEMENT REACTION WITH L-CYSTEINE - APPLICATION TO STUDIES OF WILD-TYPE AND MUTANT TRYPTOPHAN SYNTHASE ALPHA-2-BETA-2-COMPLEXES SO ANALYTICAL BIOCHEMISTRY LA English DT Article ID ESCHERICHIA-COLI; ENZYMATIC-SYNTHESIS; SUBUNIT C1 NIH, NATL INST DIABETES & DIGEST & KIDNEY DIS, BIOCHEM PHARMACOL LAB, BETHESDA, MD 20892 USA. NR 17 TC 7 Z9 7 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0003-2697 EI 1096-0309 J9 ANAL BIOCHEM JI Anal. Biochem. PD MAR PY 1991 VL 193 IS 2 BP 200 EP 203 DI 10.1016/0003-2697(91)90009-I PG 4 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA FB610 UT WOS:A1991FB61000009 PM 1872468 ER PT J AU CIARDIELLO, F KIM, N MCGEADY, ML LISCIA, DS SAEKI, T BIANCO, C SALOMON, DS AF CIARDIELLO, F KIM, N MCGEADY, ML LISCIA, DS SAEKI, T BIANCO, C SALOMON, DS TI EXPRESSION OF TRANSFORMING GROWTH-FACTOR ALPHA (TGF-ALPHA) IN BREAST-CANCER SO ANNALS OF ONCOLOGY LA English DT Review DE ESTROGENS; MAMMARY GLAND; NEU; RAS; TRANSFORMATION; TGF-ALPHA ID MAMMARY EPITHELIAL-CELLS; MESSENGER RIBONUCLEIC-ACID; FACTOR RECEPTOR; FACTOR-BETA; RAT FIBROBLASTS; EGF-RECEPTOR; CELLULAR-TRANSFORMATION; GLAND GROWTH; HA-RAS; ONCOGENES AB Transforming growth factor alpha (TGF-alpha) is one growth factor that has been circumstantially implicated in regulating the autocrine growth of breast cancer cells. Expression of TGF-alpha can be modulated by activated cellular protooncogenes such as ras and by estrogens. For example, the epidermal growth factor (EGF)-responsive normal NOG-8 mouse and human MCF-10A mammary epithelial cell lines can be transformed with either a point-mutated c-Ha-ras protooncogene or with a normal or point-mutated c-neu (erbB-2) protooncogene. In ras transformed NOG-8 and MCF-10A cells but not in neu transformed cells there is a loss in or an attenuated response to the mitogenic effects of EGF. This response may be due in part to an enhanced production of endogenous TGF-alpha that is coordinately and temporally linked to the expression of the activated ras gene and to the acquisition of transformation-associated properties in these cells. TGF-alpha mRNA and TGF-alpha protein can also be detected in approximately 50-70% of primary human breast tumors. In addition, approximately 2- to 3-fold higher levels of biologically active and immunoreactive TGF-alpha can also be detected in the pleural effusions from breast cancer patients as compared with the TGF-alpha levels in the serous effusions of noncancer patients. Over-expression of a full-length TGF-alpha cDNA in NOG-8 and MCF-10A cells is capable of transforming these cells. Finally, expression of TGF-alpha mRNA and production of biologically active TGF-alpha protein is also found in normal rodent and human mammary epithelial cells. C1 NCI,TUMOR IMMUNOL & BIOL LAB,DIV CANC BIOL & DIAG,BETHESDA,MD 20892. OI Ciardiello, Fortunato/0000-0002-3369-4841 NR 111 TC 39 Z9 39 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0923-7534 J9 ANN ONCOL JI Ann. Oncol. PD MAR PY 1991 VL 2 IS 3 BP 169 EP 182 PG 14 WC Oncology SC Oncology GA FH237 UT WOS:A1991FH23700003 PM 2043488 ER PT J AU KAKLAMANIS, P VAYOPOULOS, G STAMATELOS, G DADINAS, G TSOKOS, GC AF KAKLAMANIS, P VAYOPOULOS, G STAMATELOS, G DADINAS, G TSOKOS, GC TI CHRONIC LUPUS PERITONITIS WITH ASCITES SO ANNALS OF THE RHEUMATIC DISEASES LA English DT Article ID ERYTHEMATOSUS; COMPLICATION; SEROSITIS AB A 28 year old woman with systemic lupus erythematosus who developed chronic lupus peritonitis and ascites is described. Lupus peritonitis appeared with abdominal fullness, postprandial abdominal discomfort, and painless ascites. Four months later the patient developed vertigo, headaches, visual disturbances, serositis, and glomerulonephritis. Lupus peritonitis and the other disease manifestations responded to treatment with intravenous pulse methylprednisolone (four 1 g/m2 injections at one week intervals), oral azathioprine (200 mg daily), and diuretics. C1 NIH,KIDNEY DIS SECT,BETHESDA,MD 20892. RP KAKLAMANIS, P (reprint author), NATL UNIV ATHENS,LAIKO GEN HOSP,SCH MED,DEPT INTERNAL MED 1,17 AGHIOU THOMA ST,GR-11527 ATHENS,GREECE. NR 23 TC 34 Z9 35 U1 0 U2 1 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON, ENGLAND WC1H 9JR SN 0003-4967 J9 ANN RHEUM DIS JI Ann. Rheum. Dis. PD MAR PY 1991 VL 50 IS 3 BP 176 EP 177 DI 10.1136/ard.50.3.176 PG 2 WC Rheumatology SC Rheumatology GA EZ757 UT WOS:A1991EZ75700009 PM 2015011 ER PT J AU BROWN, PS HOLLAND, FW PARENTEAU, GL CLARK, RE AF BROWN, PS HOLLAND, FW PARENTEAU, GL CLARK, RE TI MAGNESIUM-ION IS BENEFICIAL IN HYPOTHERMIC CRYSTALLOID CARDIOPLEGIA SO ANNALS OF THORACIC SURGERY LA English DT Article ID MYOCARDIAL PRESERVATION; CALCIUM; TISSUE AB The role of magnesium ion and its relation to the calcium concentration of cardioplegic solutions was reexamined in this study. Isolated rat hearts were used with an oxygenated modified Krebs-Henseleit bicarbonate buffer as perfusion medium. The hearts were arrested for 20 minutes at 37-degrees-C or 90 minutes at 24-degrees-C. Treatment groups received one dose of nine possible cardioplegic solutions containing magnesium (0, 1.2, or 15 mmol/L) and calcium (0.05, 1.5, or 4.5 mmol/L). Ninety-six percent of the 75 magnesium-treated hearts recovere, regardless of the calcium concentraion, in contrast to a 52% recovery rate in the 69 hearts that did not receive magnesium. The addition of 15 mmol/L Mg2+ to a cardioplegic solution containing no magnesium but 0.05 mmol/L Ca2+ significantly increased (p < 0.01) the percent recovery of the following parameters of cardiac function: systolic pressure, 74% to 93% (37-degrees-C), 64% to 98% (24-degrees-C); cardiac output, 76% to 101% (37-degrees-C), 71% to 102% (24-degrees-C); stroke work, 64% to 104% (37-degrees-C), 52% to 99% (24-degrees-C); and adenosine triphosphate level, 75% to 83% (37-degrees-C), 58% to 90% (24-degrees-C). There were significant reductions (p < 0.03) in percent recovery (37-degrees-C and 24-degrees-C) of cardiac output, stroke work, and adenosine triphosphate level in the groups that contained 0 or 15 mmol/L Mg2+ as the calcium concentration was increased from 0.05 to 4.5 mmol/L. Significant progressive augmentation of ventricular recovery and decreased overall mortality is produced at 37-degrees and 24-degrees-C with cardioplegia containing high levels of magnesium and low levels of calcium. These data demonstrate the importance of the Mg2+/Ca2+ relation in an oxygenated crystalloid solution with a pH of 7.45, carbon dioxide tension of 40 mm Hg, and oxygen tension of 750 mm Hg containing 142 mmol/L Na+. C1 NHLBI,SURG BRANCH,BETHESDA,MD 20892. NR 19 TC 34 Z9 36 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0003-4975 J9 ANN THORAC SURG JI Ann. Thorac. Surg. PD MAR PY 1991 VL 51 IS 3 BP 359 EP 367 PG 9 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA FB017 UT WOS:A1991FB01700003 PM 1998412 ER PT J AU ROBERTS, CS MCINTOSH, CL BROWN, PS CANNON, RO GERTZ, SD CLARK, RE AF ROBERTS, CS MCINTOSH, CL BROWN, PS CANNON, RO GERTZ, SD CLARK, RE TI REOPERATION FOR PERSISTENT OUTFLOW OBSTRUCTION IN HYPERTROPHIC CARDIOMYOPATHY SO ANNALS OF THORACIC SURGERY LA English DT Article ID MITRAL-VALVE REPLACEMENT; SEPTAL MYOTOMY; STENOSIS; SURGERY AB This study compares results of a second left ventricular myotomy and myectomy (M+M) with those of mitral valve replacement (MVR) as reoperative procedures for persistent left ventricular outflow obstruction after M+M in hypertrophic cardiomyopathy. Comparison of the second M+M group (n = 12) with the MVR group (n = 11) disclosed significant differences (p < 0.05) in mean age at the initial operation (29 +/- 11 years versus 40 +/- 8 years), interval between operations (46 +/- 57 months versus 18 +/- 13 months), and age at reoperation (33 +/- 10 years versus 42 +/- 8 years); and insignificant differences in mean preoperative functional class, cardiac index, left ventricular outflow gradients at rest or with provocation, and hospital mortality at reoperation (2/12 versus 1/11). At 6 months after reoperation, comparison of results of a second M+M with MVR showed that mean functional class, cardiac index, and left ventricular outflow gradient at rest were similarly improved, but the outflow gradient with provocation was significantly higher in the second M+M group (57 +/- 44 mm Hg versus 14 +/- 9 mm Hg, p < 0.05). Total follow-up was 108 patient-years (100% complete) with an average of 5.9 years per patient in the second M+M group and 3.4 years per patient in the MVR group. Actuarial survival, including hospital mortality, at 3 and 5 years was 83% and 76%, respectively, after the second M+M, which was similar to 92% and 77% after MVR. Thus, either a second M+M or MVR is effective in relieving the hemodynamic obstruction and decreasing symptoms, but a second M-M is preferable because complications of anticoagulation and substitute valves are avoided. C1 NHLBI,SURG BRANCH,BETHESDA,MD 20892. NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. NHLBI,PATHOL BRANCH,BETHESDA,MD 20892. NR 13 TC 15 Z9 16 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0003-4975 J9 ANN THORAC SURG JI Ann. Thorac. Surg. PD MAR PY 1991 VL 51 IS 3 BP 455 EP 460 PG 6 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA FB017 UT WOS:A1991FB01700019 PM 1998425 ER PT J AU MEHTA, RG STEELE, V KELLOFF, GJ MOON, RC AF MEHTA, RG STEELE, V KELLOFF, GJ MOON, RC TI INFLUENCE OF THIOLS AND INHIBITORS OF PROSTAGLANDIN BIOSYNTHESIS ON THE CARCINOGEN-INDUCED DEVELOPMENT OF MAMMARY LESIONS INVITRO SO ANTICANCER RESEARCH LA English DT Article DE CHEMOPREVENTION; ORGAN CULTURE; MAMMARY GLAND; PRENEOPLASTIC LESIONS ID ORGAN-CULTURE; GLAND INVITRO; TRANSFORMATION; MODULATION; CELLS AB Mouse mammary gland undergoes physiological changes, comparable to that in the animal, under appropriate hormonal conditions in organ culture. Moreover, they form mammary lesions when exposed to the carcinogen, dimethylbenz(a)anthracene (DMBA) for a short duration. This organ culture system was utilized in the present study to evaluate activity of two groups of potential chemopreventive agents. Activity of inhibitors of prostaglandin synthesis and thiols was determined at five concentrations. The average incidence of mammary lesions induced by DMBA was 60%, oltipraz, oxithiazolidine, thioctic acid and N-acetylcysteine were effective at different concentrations, whereas diallyldisulfide, sulfasalazine and ajoene were marginally effective. Within the inhibitors of prostaglandin synthesis category, aspirin, ibuprofen and indomethacin were effective, whereas dipyridamol, piroxicam and pentoxyphyllin were ineffective. The results correlated well with the known in vivo effects of these agents on chemically induced carcinogenesis of mammary gland. The study provides evidence for the use of mammary gland organ culture as a screening tool system for prediction of effective chemopreventive agents against mammary carcinogenesis. C1 NCI,CHEMOPREVENT BRANCH,BETHESDA,MD 20892. RP MEHTA, RG (reprint author), IIT,INST RES,DEPT LIFE SCI,PATHOPHYSIOL LAB,10 W 35TH ST,CHICAGO,IL 60616, USA. FU NCI NIH HHS [CN-55502] NR 20 TC 32 Z9 33 U1 0 U2 0 PU INT INST ANTICANCER RESEARCH PI ATHENS PA EDITORIAL OFFICE 1ST KM KAPANDNTIOU-KALAMOU RD KAPANDRITI, POB 22, ATHENS 19014, GREECE SN 0250-7005 J9 ANTICANCER RES JI Anticancer Res. PD MAR-APR PY 1991 VL 11 IS 2 BP 587 EP 592 PG 6 WC Oncology SC Oncology GA FU206 UT WOS:A1991FU20600011 PM 1905901 ER PT J AU CHRISEY, L ROSSI, J SARVER, N AF CHRISEY, L ROSSI, J SARVER, N TI RIBOZYMES - PROGRESS AND PROSPECTS OF CATALYTIC RNA AS THERAPEUTIC AGENTS SO ANTISENSE RESEARCH AND DEVELOPMENT LA English DT Editorial Material C1 SYNTHECELL CORP,ROCKVILLE,MD 20850. NIAID,DIV AIDS,TARGETED DRUG DISCOVERY SECT,BETHESDA,MD 20892. CITY HOPE NATL MED CTR,BECKMAN RES INT,DUARTE,CA 91010. NR 0 TC 9 Z9 9 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1050-5261 J9 ANTISENSE RES DEV JI Antisense Res. Dev. PD SPR PY 1991 VL 1 IS 1 BP 57 EP 63 PG 7 WC Biotechnology & Applied Microbiology; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Research & Experimental Medicine GA FU886 UT WOS:A1991FU88600006 PM 1822249 ER PT J AU GOPALAKRISHNA, R ANDERSON, WB AF GOPALAKRISHNA, R ANDERSON, WB TI REVERSIBLE OXIDATIVE ACTIVATION AND INACTIVATION OF PROTEIN-KINASE-C BY THE MITOGEN TUMOR PROMOTER PERIODATE SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Note ID LYMPHOCYTE-TRANSFORMATION; ACID; RECEPTOR; PHOSPHORYLATION; DIACYLGLYCEROL; INHIBITION; BINDING; FAMILY; CELLS C1 NCI,CELLULAR ONCOL LAB,BETHESDA,MD 20892. RP GOPALAKRISHNA, R (reprint author), UNIV SO CALIF,SCH MED,DEPT PHARMACOL & NUTR,LOS ANGELES,CA 90033, USA. FU NCI NIH HHS [CA 47142] NR 32 TC 79 Z9 79 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD MAR PY 1991 VL 285 IS 2 BP 382 EP 387 DI 10.1016/0003-9861(91)90377-U PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FA647 UT WOS:A1991FA64700029 PM 1654774 ER PT J AU KORMAN, NJ STANLEY, JR WOODLEY, DT AF KORMAN, NJ STANLEY, JR WOODLEY, DT TI COEXISTENCE OF PEMPHIGUS FOLIACEUS AND BULLOUS PEMPHIGOID - DEMONSTRATION OF AUTOANTIBODIES THAT BIND TO BOTH THE PEMPHIGUS FOLIACEUS ANTIGEN COMPLEX AND THE BULLOUS PEMPHIGOID ANTIGEN SO ARCHIVES OF DERMATOLOGY LA English DT Article ID VULGARIS; PROTEIN AB Pemphigus and bullous pemphigoid are autoimmune blistering diseases of the skin characterized by circulating autoantibodies directed against the keratinocyte cell surface and the epidermal basement membrane zone, respectively. The coexistence of pemphigus and bullous pemphigoid is very uncommon. We describe a patient with pemphigus foliaceus who later developed bullous pemphigoid and show, by means of immunoprecipitation studies utilizing both cultured keratinocytes and suction blister epidermis, that our patient had circulating autoantibodies directed against both the pemphigus foliaceus antigen complex and the bullous pemphigoid antigen. This report is the first to demonstrate the coexistence of pemphigus foliaceus and bullous pemphigoid at the molecular level. C1 NCI,DERMATOL BRANCH,BETHESDA,MD 20892. UNIV N CAROLINA,DEPT DERMATOL,CHAPEL HILL,NC 27514. FU NIAMS NIH HHS [AR 0111540, R01 AR33625] NR 15 TC 34 Z9 34 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-987X J9 ARCH DERMATOL JI Arch. Dermatol. PD MAR PY 1991 VL 127 IS 3 BP 387 EP 390 DI 10.1001/archderm.127.3.387 PG 4 WC Dermatology SC Dermatology GA FA772 UT WOS:A1991FA77200013 PM 1998370 ER PT J AU LINOS, A BLAIR, A GIBSON, RW EVERETT, G VANLIER, S CANTOR, KP SCHUMAN, L BURMEISTER, L AF LINOS, A BLAIR, A GIBSON, RW EVERETT, G VANLIER, S CANTOR, KP SCHUMAN, L BURMEISTER, L TI LEUKEMIA AND NON-HODGKINS-LYMPHOMA AND RESIDENTIAL PROXIMITY TO INDUSTRIAL-PLANTS SO ARCHIVES OF ENVIRONMENTAL HEALTH LA English DT Article ID MALIGNANT-LYMPHOMA; CANCER AB The risks of developing leukemia and non-Hodgkin's lymphoma from living near industrial facilities were evaluated among men from Iowa and Minnesota in a population-based, case-control study. We found a statistically significant increase in the risk of developing non-Hodgkin's lymphoma (RR = 1.4) and a slight, nonsignificant excess for leukemia (RR = 1.2) among individuals who lived .8-3.2 km (1/2-2 miles) from a factory. Risks were greater for certain histologic types: follicular lymphoma (RR = 1.5), acute lymphocytic leukemia (RR = 5.4), and acute myelocytic leukemia (RR = 2.2). For non-Hodgkin's lymphoma (but not for leukemia), the relative risks for those living within .8 km (1/2 mile) of a factory were similar or slightly larger than for those living .8-3.2 km (1/2-2 miles) from a factory. Risks did not increase with duration of residence near a factory. The elevated risks of non-Hodgkin's lymphoma were particularly associated with residing near stone, clay, or glass industry facilities. The risk of developing leukemia was greater among persons who resided near chemical and petroleum plants. These preliminary findings raise the possibility that general environmental exposure associated with certain industrial activities may elevate the risk of developing leukemia and non-Hodgkin's lymphoma. Evaluation of data on proximity to industrial plants from studies in other geographic locations is needed to determine whether our results represent a meaningful association. C1 NCI,ENVIRONM EPIDEMIOL BRANCH EXECUT PLAZA N,ROOM 418,ROCKVILLE,MD 20892. ATHENS MED SCH,DEPT EPIDEMIOL,ATHENS,GREECE. UNIV MINNESOTA,SCH PUBL HLTH,MINNEAPOLIS,MN 55455. UNIV IOWA,DEPT PREVENT MED,IOWA CITY,IA 52242. NR 14 TC 37 Z9 37 U1 2 U2 2 PU HELDREF PUBLICATIONS PI WASHINGTON PA 1319 EIGHTEENTH ST NW, WASHINGTON, DC 20036-1802 SN 0003-9896 J9 ARCH ENVIRON HEALTH JI Arch. Environ. Health PD MAR-APR PY 1991 VL 46 IS 2 BP 70 EP 74 PG 5 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA FD803 UT WOS:A1991FD80300001 PM 2006896 ER PT J AU CANTOR, KP BOOZE, CF AF CANTOR, KP BOOZE, CF TI MORTALITY AMONG AERIAL PESTICIDE APPLICATORS AND FLIGHT INSTRUCTORS (REPRINTED FROM ARCHIVES OF ENVIRONMENTAL-HEALTH, VOL 45, PG 295-302) SO ARCHIVES OF ENVIRONMENTAL HEALTH LA English DT Article ID LUNG-CANCER; LYMPHOMA; EXPOSURE; PILOTS; RISK AB A cohort mortality study was conducted of male aerial pesticide applicators and flight instructors identified from computerized Federal Aviation Administration medical examination records for 1965-1979. Vital status of 9 677 applicators and 9 727 instructors was determined through January 1, 1980, and standardized mortality ratios (SMRs) were calculated. The overall SMR was 127 for applicators (699 deaths) and 93 for instructors (454 deaths). Fatalities from nonmotor vehicle accidents, mostly aircraft crashes, were in notable excess (SMR = 1 168 among applicators, 630 among instructors), whereas deaths from most chronic diseases, including all cancer, was below expectation (e.g., for arteriosclerotic heart disease, SMR = 52 among applicators and 50 among instructors). The ability of the study to assess cancer risk among applicators was limited by a relatively brief follow-up period. However, 8 applicators (SMR = 171), but only 1 flight instructor (SMR = 24), died of leukemia, and small, nonsignificant risk elevations for some other cancer sites among applicators were observed and warrant continued follow-up. C1 FAA,INST CIVIL AEROMED,OKLAHOMA CITY,OK 73125. RP CANTOR, KP (reprint author), NCI,ENVIRONM EPIDEMIOL BRANCH,443 EXECUT PLAZA N,BETHESDA,MD 20892, USA. NR 22 TC 23 Z9 24 U1 0 U2 0 PU HELDREF PUBLICATIONS PI WASHINGTON PA 1319 EIGHTEENTH ST NW, WASHINGTON, DC 20036-1802 SN 0003-9896 J9 ARCH ENVIRON HEALTH JI Arch. Environ. Health PD MAR-APR PY 1991 VL 46 IS 2 BP 110 EP 116 PG 7 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA FD803 UT WOS:A1991FD80300007 PM 2006895 ER PT J AU BREIER, A SCHREIBER, JL DYER, J PICKAR, D AF BREIER, A SCHREIBER, JL DYER, J PICKAR, D TI NATIONAL-INSTITUTE-OF-MENTAL-HEALTH LONGITUDINAL-STUDY OF CHRONIC-SCHIZOPHRENIA - PROGNOSIS AND PREDICTORS OF OUTCOME SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID FRONTAL LOBE LESIONS; TERM FOLLOW-UP; LONG-TERM; DEPRESSION; BEHAVIOR; ILLNESS; METHODOLOGY; PREVALENCE; DISORDERS; PSYCHOSIS AB We performed a longitudinal study of chronic schizophrenic patients who were hospitalized for research purposes at the National Institute of Mental Health (NIMH) Intramural Program in the 1970s and early 1980s. We assessed present course, outcome and predictor data from the initial cohort of 58 young chronic schizophrenic patients who were followed up for 2 to 12 years following their NIMH index hospitalization. At follow-up, the sample showed substantial functional impairment and levels of symptoms with only about 20% of the sample demonstrating a good outcome. In addition, strong intercorrelation was noted among the symptom and functioning indexes at follow-up. Moreover, neuropsychologic tests of frontal cortical functioning were significantly correlated with outcome levels of negative symptoms and social functioning but not with levels of positive symptoms. During the period from the index hospitalization to the follow-up assessment, 78% of the sample suffered a relapse, 38% attempted suicide and 24% had episodes of major affective illness. Furthermore, levels of positive and negative symptoms ascertained when patients received optimal neuroleptic treatment during the index hospitalization significantly predicted outcome levels of symptoms and functioning and time spent hospitalized during the follow-up period. In contrast, levels of index positive and negative symptoms ascertained during the drug-free state did not predict outcome symptoms or functioning. These data suggest that treatment response is a critical predictor variable. We examined the implication of these data for the course of illness in schizophrenics. C1 UNIV PITTSBURGH,DEPT PSYCHIAT,PITTSBURGH,PA 15260. NIMH,CLIN NEUROSCI BRANCH,CLIN STUDIES SECT,BETHESDA,MD 20892. RP BREIER, A (reprint author), UNIV MARYLAND,SCH MED,DEPT PSYCHIAT,MARYLAND PSYCHIAT RES CTR,POB 21247,CATONSVILLE,MD 21228, USA. NR 59 TC 463 Z9 466 U1 8 U2 34 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD MAR PY 1991 VL 48 IS 3 BP 239 EP 246 PG 8 WC Psychiatry SC Psychiatry GA FA690 UT WOS:A1991FA69000005 PM 1671741 ER PT J AU WADDINGTON, JL TORREY, EF CROW, TJ HIRSCH, SR AF WADDINGTON, JL TORREY, EF CROW, TJ HIRSCH, SR TI SCHIZOPHRENIA, NEURODEVELOPMENT, AND DISEASE - THE 5TH BIANNUAL WINTER WORKSHOP ON SCHIZOPHRENIA, BADGASTEIN, AUSTRIA, JANUARY 28 TO FEBRUARY 3, 1990 SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Editorial Material ID BRAIN C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,WASHINGTON,DC 20032. RP WADDINGTON, JL (reprint author), ROYAL COLL SURG IRELAND,DEPT CLIN PHARMACOL,ST STEPHENS GREEN,DUBLIN 2,IRELAND. RI crow, timothy/M-8327-2014 OI crow, timothy/0000-0002-5482-6655 NR 8 TC 26 Z9 28 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD MAR PY 1991 VL 48 IS 3 BP 271 EP 273 PG 3 WC Psychiatry SC Psychiatry GA FA690 UT WOS:A1991FA69000010 PM 1671744 ER PT J AU BIHARI, K PATO, MT HILL, JL MURPHY, DL AF BIHARI, K PATO, MT HILL, JL MURPHY, DL TI NEUROLOGIC SOFT SIGNS IN OBSESSIVE-COMPULSIVE DISORDER SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Letter RP BIHARI, K (reprint author), NIMH,CTR CLIN,CLIN SCI LAB,10-3 D41,BETHESDA,MD 20892, USA. FU NIMH NIH HHS [MH-00750, MH-30906] NR 3 TC 19 Z9 19 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD MAR PY 1991 VL 48 IS 3 BP 278 EP 278 PG 1 WC Psychiatry SC Psychiatry GA FA690 UT WOS:A1991FA69000011 PM 2036127 ER PT J AU SCHWARTZ, PJ AF SCHWARTZ, PJ TI REDUCED REM LATENCY IN DEPRESSION - MECHANICAL CONSIDERATIONS SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Letter RP SCHWARTZ, PJ (reprint author), NIMH,CLIN PSYCHOL BRANCH,ROOM 45239,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Schwartz, Peter/J-4267-2016 OI Schwartz, Peter/0000-0003-0367-1048 NR 6 TC 0 Z9 0 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD MAR PY 1991 VL 48 IS 3 BP 279 EP 280 PG 2 WC Psychiatry SC Psychiatry GA FA690 UT WOS:A1991FA69000013 PM 1996922 ER PT J AU SUMMERFELT, AT ALPHS, LD FUNDERBURK, FR STRAUSS, ME WAGMAN, AMI AF SUMMERFELT, AT ALPHS, LD FUNDERBURK, FR STRAUSS, ME WAGMAN, AMI TI IMPAIRED WISCONSIN-CARD-SORT PERFORMANCE IN SCHIZOPHRENIA MAY REFLECT MOTIVATIONAL DEFICITS SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Letter ID DORSOLATERAL PREFRONTAL CORTEX; PHYSIOLOGIC DYSFUNCTION C1 NIMH,SCHIZOPHRENIA RES BRANCH,BETHESDA,MD 20892. RP SUMMERFELT, AT (reprint author), MARYLAND PSYCHIAT RES CTR,POB 21247,BALTIMORE,MD 21228, USA. NR 7 TC 39 Z9 40 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD MAR PY 1991 VL 48 IS 3 BP 282 EP 283 PG 2 WC Psychiatry SC Psychiatry GA FA690 UT WOS:A1991FA69000017 PM 1996924 ER PT J AU GOLDBERG, TE WEINBERGER, DR AF GOLDBERG, TE WEINBERGER, DR TI IMPAIRED WISCONSIN-CARD-SORT PERFORMANCE IN SCHIZOPHRENIA MAY REFLECT MOTIVATIONAL DEFICITS - REPLY SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Letter ID DORSOLATERAL PREFRONTAL CORTEX; DYSFUNCTION RP GOLDBERG, TE (reprint author), NIMH,INTRAMURAL RES PROGRAM,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032, USA. NR 7 TC 6 Z9 6 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD MAR PY 1991 VL 48 IS 3 BP 283 EP 283 PG 1 WC Psychiatry SC Psychiatry GA FA690 UT WOS:A1991FA69000018 ER PT J AU FREED, WJ POLTORAK, M AF FREED, WJ POLTORAK, M TI BRAIN-TISSUE TRANSPLANTATION WITHOUT IMMUNOSUPPRESSION - COMMENT SO ARCHIVES OF NEUROLOGY LA English DT Letter RP FREED, WJ (reprint author), NIMH,NEUROSCI CTR ST ELIZABETHS,WASHINGTON,DC 20032, USA. NR 12 TC 6 Z9 6 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9942 J9 ARCH NEUROL-CHICAGO JI Arch. Neurol. PD MAR PY 1991 VL 48 IS 3 BP 259 EP 260 PG 2 WC Clinical Neurology SC Neurosciences & Neurology GA FA667 UT WOS:A1991FA66700002 PM 2001180 ER PT J AU AXIOTIS, CA MERINO, MJ AIN, K NORTON, JA AF AXIOTIS, CA MERINO, MJ AIN, K NORTON, JA TI PAPILLARY ENDOTHELIAL HYPERPLASIA IN THE THYROID FOLLOWING FINE-NEEDLE ASPIRATION SO ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE LA English DT Article ID ANGIOSARCOMA AB A 74-year-old woman was found to have exuberant papillary endothelial hyperplasia within her thyroid gland subsequent to multiple fine-needle aspiration biopsies of a Hurthle cell neoplasm. To our knowledge, this histologic alteration in the thyroid has not been previously reported. We describe this unusual complication following fine-needle aspiration, and we delineate the clinicopathologic and differential diagnostic features. C1 NATL INST DIAB & DIGEST & KIDNEY DISORDERS,CLIN ENDOCRINOL BRANCH,BETHESDA,MD. NCI,DEPT PATHOL,BETHESDA,MD 20892. NCI,SURG BRANCH,BETHESDA,MD 20892. RP AXIOTIS, CA (reprint author), NCI,CTR CLIN,BLDG 10,ROOM 2N212,BETHESDA,MD 20892, USA. RI Ain, Kenneth/A-5179-2012 OI Ain, Kenneth/0000-0002-2668-934X NR 11 TC 40 Z9 40 U1 0 U2 0 PU COLLEGE AMER PATHOLOGISTS PI NORTHFIELD PA C/O KIMBERLY GACKI, 325 WAUKEGAN RD, NORTHFIELD, IL 60093-2750 SN 0003-9985 J9 ARCH PATHOL LAB MED JI Arch. Pathol. Lab. Med. PD MAR PY 1991 VL 115 IS 3 BP 240 EP 242 PG 3 WC Medical Laboratory Technology; Medicine, Research & Experimental; Pathology SC Medical Laboratory Technology; Research & Experimental Medicine; Pathology GA FA666 UT WOS:A1991FA66600014 PM 2001162 ER PT J AU REYBURN, TV AF REYBURN, TV TI THE Z STRAP - HARNESSING MODIFICATION FOR PATIENTS WITH UPPER EXTREMITY AMPUTATIONS SO ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION LA English DT Article DE AMPUTATION; ARM; ARTIFICIAL LIMB; PROSTHESIS AB High level, upper extremity amputees have been observed to have problems affecting overall prosthetic function. These include: tendency of the Northwestern Ring to ride upward and difficulty with terminal device function above 90 degrees of elbow flexion. A simple modification of the control strap was tried to resolve these problems of function. The control strap was attached across the back to a buckle on the unamputated side at the shoulder to provide a more efficient anchor. This created more body contact across the back and formed a type of "Z" strap allowing full freedom of movement in any direction. Less forward shoulder flexion was necessary due to decreased cable travel and because the strap was not sliding up and over the shoulder. A single case study is described and the successful outcome for the amputee. RP REYBURN, TV (reprint author), NIH,DEPT REHABIL MED,OCCUPAT THERAPY SERV,BLDG 10,ROOM 6S235,BETHESDA,MD 20892, USA. NR 4 TC 1 Z9 1 U1 0 U2 4 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0003-9993 J9 ARCH PHYS MED REHAB JI Arch. Phys. Med. Rehabil. PD MAR PY 1991 VL 72 IS 3 BP 250 EP 252 PG 3 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA FA664 UT WOS:A1991FA66400017 PM 1998464 ER PT J AU SINDELAR, WF DELANEY, TF TOCHNER, Z THOMAS, GF DACHOSWKI, LJ SMITH, PD FRIAUF, WS COLE, JW GLATSTEIN, E AF SINDELAR, WF DELANEY, TF TOCHNER, Z THOMAS, GF DACHOSWKI, LJ SMITH, PD FRIAUF, WS COLE, JW GLATSTEIN, E TI TECHNIQUE OF PHOTODYNAMIC THERAPY FOR DISSEMINATED INTRAPERITONEAL MALIGNANT NEOPLASMS - PHASE-I STUDY SO ARCHIVES OF SURGERY LA English DT Article; Proceedings Paper CT 43RD ANNUAL CANCER SYMP OF THE SOC OF SURGICAL ONCOLOGY CY MAY 20, 1990 CL WASHINGTON, DC SP SOC SURG ONCOL ID PHOTORADIATION; CARCINOMA AB Patients with disseminated intraperitoneal malignant neoplasms were given intra-abdominal photodynamic therapy. Patients received dihematoporphyrin ethers intravenously 48 to 72 hours before laparotomy at doses of 1.5 to 3.0 mg/kg. At operation, as much tumor as possible was resected. Red light (630 nm) was delivered to all peritoneal surfaces from an argon-pumped dye laser at doses ranging from 0.2 to 3.0 J/cm2 in an escalating fashion. Viscera and peritoneal surfaces were anatomically isolated and exposed to light for intervals calculated to deliver the prescribed energy. Light was delivered to mesentery and bowel by a flat-cut optical fiber, while other areas, including diaphragm, viscera, omental bursa, gutters, and pelvis, were delivered light through a diffusing wand. Twenty-three patients (13 with ovarian cancer, eight with sarcoma, and two with pseudomyxoma peritoneii) underwent photodynamic therapy. Five of eight patients cleared positive peritoneal cytologies after treatment. Six patients remained clinically free of disease for up to 18 months, and five patients had treatment-related complications. Intraperitoneal phototherapy is technically feasible and deserving of clinical evaluation. C1 NCI,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. RP SINDELAR, WF (reprint author), NCI,SURG BRANCH,BLDG 10,ROOM 2B38,BETHESDA,MD 20892, USA. NR 11 TC 97 Z9 100 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0004-0010 J9 ARCH SURG-CHICAGO JI Arch. Surg. PD MAR PY 1991 VL 126 IS 3 BP 318 EP 324 PG 7 WC Surgery SC Surgery GA FA603 UT WOS:A1991FA60300010 PM 1998474 ER PT J AU MARGITIC, SE BOND, MG CROUSE, JR FURBERG, CD PROBSTFIELD, JL AF MARGITIC, SE BOND, MG CROUSE, JR FURBERG, CD PROBSTFIELD, JL TI PROGRESSION AND REGRESSION OF CAROTID ATHEROSCLEROSIS IN CLINICAL-TRIALS SO ARTERIOSCLEROSIS AND THROMBOSIS LA English DT Editorial Material DE MEETING SUMMARY; CAROTID ATHEROSCLEROSIS; CLINICAL TRIALS; ULTRASONOGRAPHY ID B-MODE ULTRASOUND; CORONARY ATHEROSCLEROSIS; ARTERY STENOSIS; NATURAL-HISTORY; WALL THICKNESS; RISK-FACTORS; DISEASE; ARTERIOGRAPHY; ENDARTERECTOMY; ENLARGEMENT C1 NHLBI, DIV EPIDEMIOL & CLIN APPLICAT, BETHESDA, MD 20892 USA. WAKE FOREST UNIV, BOWMAN GRAY SCH MED, DIV PREVENT CARDIOL, WINSTON SALEM, NC 27103 USA. WAKE FOREST UNIV, BOWMAN GRAY SCH MED, DIV VASC ULTRASOUND RES, WINSTON SALEM, NC 27103 USA. RP MARGITIC, SE (reprint author), WAKE FOREST UNIV, BOWMAN GRAY SCH MED, DEPT PUBL HLTH SCI, 300 S HAWTHORNE RD, WINSTON SALEM, NC 27103 USA. NR 49 TC 50 Z9 53 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 USA SN 1049-8834 J9 ARTERIOSCLER THROMB JI Arterioscler. Thromb. PD MAR-APR PY 1991 VL 11 IS 2 BP 443 EP 451 PG 9 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA FC067 UT WOS:A1991FC06700027 PM 1998661 ER PT J AU LICINIO, J GOLD, PW AF LICINIO, J GOLD, PW TI ROLE OF CORTICOTROPIN RELEASING HORMONE 41 IN DEPRESSIVE-ILLNESS SO BAILLIERES CLINICAL ENDOCRINOLOGY AND METABOLISM LA English DT Article ID SECRETION INVITRO; BIOCHEMICAL MANIFESTATIONS; NEUROBIOLOGY; BLOCKADE; NEURONS; STRESS; BRAIN C1 YALE UNIV,SCH MED,DEPT PSYCHIAT,NEW HAVEN,CT 06510. NIMH,CLIN NEUROENDOCRINOL BRANCH,BETHESDA,MD 20892. RP LICINIO, J (reprint author), W HAVEN VET AFFAIRS MED CTR,AFFECT DISORDERS UNIT,W HAVEN,CT 06516, USA. RI Licinio, Julio/L-4244-2013 OI Licinio, Julio/0000-0001-6905-5884 NR 34 TC 12 Z9 12 U1 0 U2 0 PU BAILLIERE TINDALL PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0950-351X J9 BAILLIERE CLIN ENDOC JI Baillieres Clin. Endocrinol. Metab. PD MAR PY 1991 VL 5 IS 1 BP 51 EP 58 DI 10.1016/S0950-351X(05)80096-5 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FC074 UT WOS:A1991FC07400005 PM 2039428 ER PT J AU INGRAHAM, LJ AF INGRAHAM, LJ TI A NEUROPSYCHOLOGY OF PSYCHOSIS SO BEHAVIORAL AND BRAIN SCIENCES LA English DT Article ID SCHIZOPHRENIA RP INGRAHAM, LJ (reprint author), NIMH,PSYCHOL & PSYCHOPATHOL LAB,BLDG 10,ROOM 4C110,BETHESDA,MD 20892, USA. NR 9 TC 0 Z9 0 U1 0 U2 0 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 0140-525X J9 BEHAV BRAIN SCI JI Behav. Brain Sci. PD MAR PY 1991 VL 14 IS 1 BP 34 EP 34 PG 1 WC Psychology, Biological; Behavioral Sciences; Neurosciences SC Psychology; Behavioral Sciences; Neurosciences & Neurology GA FC750 UT WOS:A1991FC75000017 ER PT J AU STEVENS, JR GOLD, JM AF STEVENS, JR GOLD, JM TI WHAT IS SCHIZOPHRENIA SO BEHAVIORAL AND BRAIN SCIENCES LA English DT Article ID HIPPOCAMPAL; DOPAMINE; RAT RP STEVENS, JR (reprint author), NIMH,NEUROSCI RES CTR,WASHINGTON,DC 20009, USA. NR 17 TC 2 Z9 2 U1 1 U2 1 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 0140-525X J9 BEHAV BRAIN SCI JI Behav. Brain Sci. PD MAR PY 1991 VL 14 IS 1 BP 50 EP 50 PG 1 WC Psychology, Biological; Behavioral Sciences; Neurosciences SC Psychology; Behavioral Sciences; Neurosciences & Neurology GA FC750 UT WOS:A1991FC75000033 ER PT J AU TANDON, NN HOLLAND, EA KRALISZ, U KLEINMAN, HK ROBEY, FA JAMIESON, GA AF TANDON, NN HOLLAND, EA KRALISZ, U KLEINMAN, HK ROBEY, FA JAMIESON, GA TI INTERACTION OF HUMAN PLATELETS WITH LAMININ AND IDENTIFICATION OF THE 67-KDA LAMININ RECEPTOR ON PLATELETS SO BIOCHEMICAL JOURNAL LA English DT Article ID CELL-ADHESION RECEPTORS; GLYCOPROTEIN-IV CD36; FIBRO-SARCOMA CELLS; MEMBRANE GLYCOPROTEIN; TUMOR-CELLS; BINDING PROTEIN; CARCINOMA-CELLS; COLLAGEN; SURFACE; COMPLEX AB A microtitre adhesion assay has been developed to define parameters affecting the adherence of washed platelets to laminin. Adherence was optimally supported by Mg2+ and was inhibited by Ca2+ and by anti-laminin Fab fragments, but significant adhesion (75-90% of control) was found both in heparinized plasma containing physiological levels of bivalent cations and in plasma anti-coagulated with EGTA. Adherence was unaffected by platelet activation with ADP but was decreased by 50% by treatment with alpha-thrombin (1 unit/ml, 5 min). Adherence was unaffected by monospecific polyclonal antibodies to glycoprotein (GP) Ib and GPIV, and was normal with platelets from two patients with Glanzmann's thrombasthaenia, indicating that GPIb, the GPIIb/IIIa complex and GPIV are not involved in platelet-laminin interaction. Affinity chromatography of Triton-solubilized membranes on laminin-Sepharose followed by elution with 0.2 M-glycine/HCl (pH 2.85) identified a major band with a molecular mass of 67 kDa in the reduced and of 53 kDa in the unreduced form. This protein gave a positive reaction on Western blotting with a monospecific polyclonal antibody raised against the high-affinity laminin receptor isolated from human breast carcinoma tissue. The adhesion of platelets to laminin was inhibited by two monoclonal IgM antibodies specific to the LR-1 domain of the 67 kDa receptor. The binding protein was surface-oriented, as shown by flow cytofluorimetry and by the fact that it could be iodinated in intact platelets, but it was not labelled by the periodate-borotritide procedure, suggesting that it did not contain terminal sialic acid. The laminin-derived peptides Tyr-Ile-Gly-Ser-Arg and Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH2, which constitute a complementary binding domain in laminin for the 67 kDa receptor, themselves supported platelet adhesion, bound to the receptor and inhibited the adhesion of platelets to laminin. In addition, Fab fragments of anti-Tyr-Ile-Gly-Ser-Arg antibody inhibited platelet adhesion to laminin. These results demonstrate that the high-affinity 67 kDa laminin receptor previously identified in a range of normal and transformed cells and its complementary Tyr-Ile-Gly-Ser-Arg binding domain play an important role in the interaction of platelets with laminin. C1 NIDR,BETHESDA,MD 20892. RP TANDON, NN (reprint author), AMER RED CROSS,CELL BIOL LAB,ROCKVILLE,MD 20855, USA. FU NCI NIH HHS [CA43768]; NHLBI NIH HHS [HL40858] NR 45 TC 30 Z9 30 U1 0 U2 1 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD MAR 1 PY 1991 VL 274 BP 535 EP 542 PN 2 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FB955 UT WOS:A1991FB95500035 PM 1826081 ER PT J AU MENNITI, FS PUTNEY, JW AF MENNITI, FS PUTNEY, JW TI INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE ACTIVITY IN HIGH-SPEED SUPERNATANTS FROM RAT PANCREATOMA CELLS, AR4-2J SO BIOCHEMICAL JOURNAL LA English DT Letter ID PHOSPHATES; METABOLISM RP MENNITI, FS (reprint author), NIEHS,CELLULAR & MOLEC PHARMACOL LAB,CALCIUM REGULAT SECT,RES TRIANGLE PK,NC 27709, USA. OI Menniti, Frank/0000-0003-2612-9534 NR 9 TC 3 Z9 3 U1 0 U2 0 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD MAR 1 PY 1991 VL 274 BP 622 EP 623 PN 2 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FB955 UT WOS:A1991FB95500051 PM 1848762 ER PT J AU BORING, DL JI, XD ZIMMET, J TAYLOR, KE STILES, GL JACOBSON, KA AF BORING, DL JI, XD ZIMMET, J TAYLOR, KE STILES, GL JACOBSON, KA TI TRIFUNCTIONAL AGENTS AS A DESIGN STRATEGY FOR TAILORING LIGAND PROPERTIES - IRREVERSIBLE INHIBITORS OF A1 ADENOSINE RECEPTORS SO BIOCONJUGATE CHEMISTRY LA English DT Article ID HIGH-AFFINITY; PURIFICATION; DERIVATIVES; ANTAGONISTS; MEMBRANES; PROTEINS AB The 1,3-phenylene diisothiocyanate conjugate of XAC (8-[4-[[[[(2-aminoethyl)amino]carbonyl]methyl]oxy] phenyl]-1,3-dipropylxanthine, a potent A1 selective adenosine antagonist) has been characterized as an irreversible inhibitor of A1 adenosine receptors. To further extend this work, a series of analogues were prepared containing a third substituent in the phenyl isothiocyanate ring, incorporated to modify the physiochemical or spectroscopic properties of the conjugate. Symmetrical trifunctional cross-linking reagents bearing two isothiocyanate groups were prepared as general intermediates for cross-linking functionalized congeners and receptors. Xanthine isothiocyanate derivatives containing hydrophilic, fluorescent, or reactive substituents, linked via an amide, thiourea, or methylene group in the 5-position, were synthesized and found to be irreversible inhibitors of Al adenosine receptors. The effects of the 5-substituent on water solubility and on the A1/A2 selectivity ratio derived from binding assays in rat brain membranes were examined. Inhibition of binding of [H-3]-N6-(2-phenylisopropyl)adenosine and [H-3]CGS21680 (2-[[2-[4-(2-carboxyethyl)phenyl]ethyl]amino]adenosine-5'-N-ethylcarboxamide) at central A1 and A2 adenosine receptors, respectively, was measured. A conjugate of XAC and 1,3,5-triisothiocyanatobenzene was 894-fold selective for A1 receptors. Reporter groups, such as fluorescent dyes and a spin-label, were included as chain substituents in the irreversibly binding analogues, which were designed for spectroscopic assays, histochemical characterization, and biochemical characterization of the receptor protein. C1 NIDDKD,BIOORGAN CHEM LAB,BETHESDA,MD 20892. DUKE UNIV,MED CTR,DEPT MED,DURHAM,NC 27710. DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z01 DK031115-24] NR 27 TC 12 Z9 12 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 1043-1802 J9 BIOCONJUGATE CHEM JI Bioconjugate Chem. PD MAR-APR PY 1991 VL 2 IS 2 BP 77 EP 88 DI 10.1021/bc00008a002 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Multidisciplinary; Chemistry, Organic SC Biochemistry & Molecular Biology; Chemistry GA GB084 UT WOS:A1991GB08400002 PM 1868116 ER PT J AU HOCHGESCHWENDER, U BRENNAN, MB AF HOCHGESCHWENDER, U BRENNAN, MB TI IDENTIFYING GENES WITHIN THE GENOME - NEW WAYS FOR FINDING THE NEEDLE IN A HAYSTACK SO BIOESSAYS LA English DT Article ID SEQUENCES; LIBRARY; CDNA; DNA RP HOCHGESCHWENDER, U (reprint author), NIMH,CLIN NEUROGENET BRANCH,GENOM UNIT,BLDG 10,ROOM 4N320,BETHESDA,MD 20892, USA. NR 19 TC 16 Z9 16 U1 0 U2 0 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL SN 0265-9247 J9 BIOESSAYS JI Bioessays PD MAR PY 1991 VL 13 IS 3 BP 139 EP 144 DI 10.1002/bies.950130308 PG 6 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA FD385 UT WOS:A1991FD38500007 PM 1872824 ER PT J AU STARR, CM HANOVER, JA AF STARR, CM HANOVER, JA TI A COMMON STRUCTURAL MOTIF IN NUCLEAR-PORE PROTEINS (NUCLEOPORINS) SO BIOESSAYS LA English DT Letter ID TRANSPORT; COMPLEX; IMPORT RP STARR, CM (reprint author), NIH,BIOCHEM & METAB LAB,BLDG 10,RM 9B 15,BETHESDA,MD 20892, USA. NR 12 TC 25 Z9 25 U1 0 U2 0 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL SN 0265-9247 J9 BIOESSAYS JI Bioessays PD MAR PY 1991 VL 13 IS 3 BP 145 EP 146 DI 10.1002/bies.950130309 PG 2 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA FD385 UT WOS:A1991FD38500008 PM 1872825 ER PT J AU TOMER, KB DETERDING, LJ GUENAT, C AF TOMER, KB DETERDING, LJ GUENAT, C TI COLLISIONALLY ACTIVATED DISSOCIATION SPECTRA OF SODIATED PEPTIDES AND PEPTIDE AMIDES SO BIOLOGICAL MASS SPECTROMETRY LA English DT Article ID TANDEM MASS-SPECTROMETRY; ALKALI-METAL-IONS; SEQUENCE IONS; FRAGMENTATION AB The CAD spectra of the [M + Na]+ ions of a series of peptide free acids and amides have been acquired. The spectra of the sodiated amides were found to differ significantly from those of the sodiated free acids due to absence of the [B(n) + Na + OH]+ ions in the spectra of the amides. The abundances of [Y(n) + Na - H]+ ions, [W(n) + Na]+ ions and [D(n) + Na]+ ions is increased in the spectra of the sodiated peptides while internal fragments are reduced in abundance. Deuterium labeling shows that the hydrogens lost in the formation of [Y(n) + Na - H]+ ions and the [A(n) + Na - H]+ ions are non-exchangeable. RP TOMER, KB (reprint author), NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. RI Tomer, Kenneth/E-8018-2013 NR 19 TC 33 Z9 33 U1 0 U2 3 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 1052-9306 J9 BIOL MASS SPECTROM JI Biol. Mass Spectrom. PD MAR PY 1991 VL 20 IS 3 BP 121 EP 129 DI 10.1002/bms.1200200305 PG 9 WC Biophysics; Spectroscopy SC Biophysics; Spectroscopy GA FC910 UT WOS:A1991FC91000004 ER PT J AU PIGOTT, TA ZOHAR, J HILL, JL BERNSTEIN, SE GROVER, GN ZOHARKADOUCH, RC MURPHY, DL AF PIGOTT, TA ZOHAR, J HILL, JL BERNSTEIN, SE GROVER, GN ZOHARKADOUCH, RC MURPHY, DL TI METERGOLINE BLOCKS THE BEHAVIORAL AND NEUROENDOCRINE EFFECTS OF ORALLY-ADMINISTERED M-CHLOROPHENYLPIPERAZINE IN PATIENTS WITH OBSESSIVE-COMPULSIVE DISORDER SO BIOLOGICAL PSYCHIATRY LA English DT Article ID SEROTONERGIC RESPONSIVITY; CLOMIPRAMINE TREATMENT; NEURO-ENDOCRINE; FLUVOXAMINE; MECHANISM; AGONIST; HUMANS; MCPP AB The pharmacological probe, meta-chlorophenylpiperazine (m-CPP), administered orally, to patients with obsessive-compulsive disorder (OCD) has been shown to induce an acute exacerbation in OCD symptoms as well as an exaggerated anxiogenic response in comparison with controls. The mechanism of m-CPP's behavioral effects in humans remains controversial. To further study m-CPP's actions in OCD patients, we completed a series of double-blind pharmacological challenges in 12 OCD patients. Six OCD patients received four separate challenges: placebo, metergoline, m-CPP, and metergoline plus m-CPP; the second group (n = 6) received metergoline and metergoline plus m-CPP in separate challenges. OCD patients receiving placebo or metergoline alone failed to show evidence of significant changes on any of the behavioral rating scales, in contrast to the patients who received m-CPP alone who exhibited significant increases in anxiety and OCD symptoms. However, the 12 OCD patients who received pretreatment with metergoline before m-CPP experienced no significant changes from baseline OCD symptoms or other behavioral changes. m-CPP's ability to elicit elevations in plasma prolactin was blocked by metergoline pretreatment. Metergoline's ability to block m-CPP's effects on behavior and plasma prolactin lends further support to a serotonergic mediation of m-CPP's effects, including its elicitation of OCD symptoms. C1 BEER SHEVA MENTAL HLTH CTR,BEER SHEVA,ISRAEL. NIH,CTR CLIN,DEPT NURSING,BETHESDA,MD 20892. RP PIGOTT, TA (reprint author), NIH,CTR CLIN,CLIN NEUROPHARMACOL SECT,CLIN SCI LAB,10-3D41,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 26 TC 91 Z9 93 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD MAR 1 PY 1991 VL 29 IS 5 BP 418 EP 426 DI 10.1016/0006-3223(91)90264-M PG 9 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA FB949 UT WOS:A1991FB94900002 PM 2018816 ER PT J AU WEINBERGER, DR AF WEINBERGER, DR TI HIPPOCAMPAL INJURY AND CHRONIC-SCHIZOPHRENIA SO BIOLOGICAL PSYCHIATRY LA English DT Letter ID DORSOLATERAL PREFRONTAL CORTEX; BLOOD-FLOW; DYSFUNCTION; PATHOLOGY; LOBE RP WEINBERGER, DR (reprint author), NIMH,ST ELIZABETHS HOSP,CTR NEUROSCI,DIRP,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032, USA. NR 8 TC 8 Z9 8 U1 2 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD MAR 1 PY 1991 VL 29 IS 5 BP 509 EP 510 DI 10.1016/0006-3223(91)90278-T PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA FB949 UT WOS:A1991FB94900016 PM 2018826 ER PT J AU PIEGORSCH, WW AF PIEGORSCH, WW TI MULTIPLE COMPARISONS FOR ANALYZING DICHOTOMOUS RESPONSE SO BIOMETRICS LA English DT Article DE BINOMIAL DISTRIBUTION; COMPARISONS WITH A CONTROL; CONFIDENCE INTERVAL; MONTE-CARLO EVALUATIONS; PAIRWISE COMPARISONS; QUANTAL RESPONSE; SIMULTANEOUS INFERENCE ID SIMULTANEOUS CONFIDENCE-INTERVALS; MULTINOMIAL PROPORTIONS AB Dichotomous response models are common in many experimental settings. Statistical parameters of interest are typically the probabilities, p(i), that an experimental unit will respond at the various treatment levels. Herein, simultaneous procedures are considered for multiple comparisons among these probabilities, with attention directed at construction of simultaneous confidence intervals for various functions of the p(i). The inferences are based on the asymptotic normality of the maximum likelihood estimator of p(i). Specific applications include all pairwise comparisons and comparisons with a fixed (control) treatment. Monte Carlo evaluations are undertaken to examine the small-sample properties of the various procedures. It is seen that use of the usual estimates of variance consistently leads to less-than-nominal empirical coverage for most sample sizes examined. For very large samples (total size greater than about 300), nominal coverage is achieved. A reformulation of the pairwise comparisons using a construction noted by Beal (1987, Biometrics 43, 941-950) is shown to exhibit generally nominal empirical coverage characteristics, and is recommended for use with small-to-moderate sample sizes. RP PIEGORSCH, WW (reprint author), NIEHS,STAT & BIOMATH BRANCH,RES TRIANGLE PK,NC 27709, USA. OI Piegorsch, Walter/0000-0003-2725-5604 NR 24 TC 25 Z9 25 U1 0 U2 4 PU INTERNATIONAL BIOMETRIC SOC PI WASHINGTON PA 808 17TH ST NW SUITE 200, WASHINGTON, DC 20006-3910 SN 0006-341X J9 BIOMETRICS JI Biometrics PD MAR PY 1991 VL 47 IS 1 BP 45 EP 52 DI 10.2307/2532494 PG 8 WC Biology; Mathematical & Computational Biology; Statistics & Probability SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology; Mathematics GA FF084 UT WOS:A1991FF08400005 PM 2049512 ER PT J AU WACHOLDER, S GAIL, M PEE, D AF WACHOLDER, S GAIL, M PEE, D TI SELECTING AN EFFICIENT DESIGN FOR ASSESSING EXPOSURE-DISEASE RELATIONSHIPS IN AN ASSEMBLED COHORT SO BIOMETRICS LA English DT Article DE CASE-COHORT DESIGN; NESTED CASE-CONTROL DESIGN; PROPORTIONAL HAZARDS; SUPERPOPULATION VARIANCE; UNBIASED CONTROL SAMPLING ID PARTIAL LIKELIHOOD; RELATIVE RISK; MODELS; TRIALS AB We develop approximate methods to compare the efficiencies and to compute the power of alternative potential designs for sampling from a cohort before beginning to collect exposure data. Our methods require only that the cohort be assembled, meaning that the numbers of individuals N(kj) at risk at pairs of event times t(k) and t(j) greater-than-or-equal-to t(k) are available. To compute N(kj), one needs to know the entry, follow-up, censoring, and event history, but not the exposure, for each individual. Our methods apply to any "unbiased control sampling design," in which cases are compared to a random sample of noncases at risk at the time of an event. We apply our methods to approximate the efficiencies of the nested case-control design, the case-cohort design, and an augmented case-cohort design, compared to the full cohort design, in an assembled cohort of 17,633 members of an insurance cooperative who were followed for mortality from prostatic cancer. The assumptions underlying the approximation are that exposure is unrelated both to the hazard of an event and to the hazard for censoring. The approximations performed well in simulations when both assumptions held and when the exposure was moderately related to censoring. C1 INFORMAT MANAGEMENT SERV INC,ROCKVILLE,MD 20852. RP WACHOLDER, S (reprint author), NCI,BIOSTAT BRANCH,6130 EXECUT BLVD,EPN-403,ROCKVILLE,MD 20892, USA. NR 21 TC 34 Z9 34 U1 1 U2 3 PU INTERNATIONAL BIOMETRIC SOC PI WASHINGTON PA 808 17TH ST NW SUITE 200, WASHINGTON, DC 20006-3910 SN 0006-341X J9 BIOMETRICS JI Biometrics PD MAR PY 1991 VL 47 IS 1 BP 63 EP 76 DI 10.2307/2532496 PG 14 WC Biology; Mathematical & Computational Biology; Statistics & Probability SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology; Mathematics GA FF084 UT WOS:A1991FF08400007 PM 2049514 ER PT J AU ANDERSON, KM AF ANDERSON, KM TI A NONPROPORTIONAL HAZARDS WEIBULL ACCELERATED FAILURE TIME REGRESSION-MODEL SO BIOMETRICS LA English DT Note DE ACCELERATED FAILURE TIME; CENSORED SURVIVAL DATA; GENERALIZED LINEAR MODELS; MAXIMUM LIKELIHOOD; PARAMETRIC MODELS; PROPORTIONAL HAZARDS; REGRESSION; VARIABLE DISPERSION; WEIBULL DISTRIBUTION ID CENSORED SURVIVAL-DATA; PROPORTIONAL HAZARDS; LIFE; VARIANCE; DISEASE AB We present a study of risk factors measured in men before age 50 and subsequent incidence of heart disease over 32 years of follow-up. The data are from the Framingham Heart Study. The standard accelerated failure time model assumes the logarithm of time until an event has a constant dispersion parameter and a location parameter that is a linear function of covariates. Parameters are estimated by maximum likelihood. We reject a standard Weibull model for these data in favor of a model with the dispersion parameter depending on the location parameter. This model suggests that the cumulative hazard ratio for two individuals shrinks toward unity over the follow-up period. Thus, not only the standard Weibull, but also the semiparametric proportional hazards (Cox) model is inadequate for this data. The model improvement appears particularly valuable when estimating the difference in predicted outcome probabilities for two individuals. C1 NHLBI,FRAMINGHAM,MA. NR 26 TC 31 Z9 32 U1 1 U2 4 PU INTERNATIONAL BIOMETRIC SOC PI WASHINGTON PA 808 17TH ST NW SUITE 200, WASHINGTON, DC 20006-3910 SN 0006-341X J9 BIOMETRICS JI Biometrics PD MAR PY 1991 VL 47 IS 1 BP 281 EP 288 DI 10.2307/2532512 PG 8 WC Biology; Mathematical & Computational Biology; Statistics & Probability SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology; Mathematics GA FF084 UT WOS:A1991FF08400023 PM 2049501 ER PT J AU SHERMAN, A RINZEL, J AF SHERMAN, A RINZEL, J TI MODEL FOR SYNCHRONIZATION OF PANCREATIC BETA-CELLS BY GAP JUNCTION COUPLING SO BIOPHYSICAL JOURNAL LA English DT Article ID INDUCED ELECTRICAL-ACTIVITY; ISLET CELLS; INSULIN RELEASE; B-CELLS; MOUSE ISLETS; LANGERHANS; CALCIUM; OSCILLATIONS; CHANNEL; CLUSTERS AB Pancreatic beta-cells coupled by gap junctions in sufficiently large clusters exhibit regular electrical bursting activity, which is described by the Chay-Keizer model and its variants. According to most reports, however, isolated cells exhibit disorganized spiking. We have previously (Sherman, A., J. Rinzel, and J. Keizer. 1988. Biophys. J. 54:411-425) modeled these behaviors by hypothesizing that stochastic channel fluctuations disrupt the bursts. We showed that when cells are coupled by infinite conductance gap junctions, so that the cluster is isopotential and may be viewed as a single "supercell," the fluctuations are shared over a larger membrane area and hence dampened. Bursting emerges when there are more than approximately 50 cells in the cluster. In the model the temporal organization of spikes into bursts increases the amplitude of intracellular calcium oscillations, which may be relevant for insulin secretion. We now extend the previous work by considering the case of a true "multicell" model with finite gap junctional conductance. Whereas the previous study assumed that the cells were synchronized, we can now study the process of synchronization itself. We show that, for sufficiently large clusters, the cells both synchronize and begin to burst with moderate, physiologically reasonable gap junctional conductance. An unexpected finding is that the burst period is longer, and calcium amplitude greater, than when coupling is infinitely strong, with an optimum in the range of 150-250 pS. Our model is in good agreement with recent experimental data of Perez-Armendariz, M., D. C. Spray, and M. V. L. Bennett. (1991. Biophys. J. 59:76-92) showing extensive gap junctions in beta-cell pairs with mean interfacial conductance of 213 +/- 113 pS. The optimality property of our model is noteworthy because simple slow-wave models without spikes do not show the same behavior. RP SHERMAN, A (reprint author), NIDDKD,MATH RES BRANCH,BLDG 31,ROOM 4B-54,BETHESDA,MD 20892, USA. NR 45 TC 130 Z9 132 U1 0 U2 5 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD MAR PY 1991 VL 59 IS 3 BP 547 EP 559 PG 13 WC Biophysics SC Biophysics GA EY790 UT WOS:A1991EY79000005 PM 1646657 ER PT J AU BENVENISTE, M MAYER, ML AF BENVENISTE, M MAYER, ML TI KINETIC-ANALYSIS OF ANTAGONIST ACTION AT N-METHYL-D-ASPARTIC ACID RECEPTORS - 2 BINDING-SITES EACH FOR GLUTAMATE AND GLYCINE SO BIOPHYSICAL JOURNAL LA English DT Article ID MOUSE CENTRAL NEURONS; ACETYLCHOLINE-RECEPTOR; HIPPOCAMPAL-NEURONS; XENOPUS-OOCYTES; ION CHANNELS; RAT; PHARMACOLOGY; ACTIVATION; CURRENTS; CULTURES AB Antagonism of glutamate-receptor responses activated by N-methyl-D-aspartic acid (NMDA) was studied using whole cell voltage clamp recording from mouse dissociated hippocampal neurons cultured for 10-15 d. The kinetics of onset of and recovery from NMDA receptor block during continuous application of NMDA together with either glycine, or L-alanine, were recorded in response to concentration jump application of NMDA- and glycine-binding site directed competitive antagonists, applied with a multibarrel flow pipe under conditions which allowed rapid solution changes around the cell < 10 ms. Mathematical solutions for both one- and two-equivalent site models for competitive antagonism were determined according to the differential equations outlined by Colquhoun and Hawkes 1977. Proc. R. Soc. Lond. B. 199:231-262). The kinetics of action of D-CPP and D-AP5, NMDA binding site antagonists, and 7Cl-kynurenic acid, a glycine binding site antagonist, were examined for each model. For all these antagonists, the kinetics for the onset of and recovery from antagonism were better fit by the two-equivalent site model, which yielded antagonist microscopic k(Boff)/k(Bon) values which closely approximated K(i) values determined from analysis of equilibrium dose response curves. These results suggest that two molecules of NMDA and two molecules of glycine must bind to the NMDA receptor for activation of ion channel gating. RP BENVENISTE, M (reprint author), NICHHD,NEUROPHYSIOL & BIOPHYS UNIT,DEV NEUROBIOL LAB,BLDG 36,ROOM 2A21,BETHESDA,MD 20892, USA. RI Mayer, Mark/H-5500-2013 NR 37 TC 144 Z9 148 U1 0 U2 3 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD MAR PY 1991 VL 59 IS 3 BP 560 EP 573 PG 14 WC Biophysics SC Biophysics GA EY790 UT WOS:A1991EY79000006 PM 1710938 ER PT J AU DEAN, M GERRARD, B AF DEAN, M GERRARD, B TI HELPFUL HINTS FOR THE DETECTION OF SINGLE-STRANDED CONFORMATION POLYMORPHISMS SO BIOTECHNIQUES LA English DT Note RP DEAN, M (reprint author), PROGRAM RESOURCES DYN CORP,FREDERICK CANC RES & DEV CTR,POB B,FREDERICK,MD 21702, USA. OI Dean, Michael/0000-0003-2234-0631 FU NCI NIH HHS [N01-CO-74102] NR 3 TC 18 Z9 18 U1 0 U2 0 PU EATON PUBLISHING CO PI NATICK PA 154 E. CENTRAL ST, NATICK, MA 01760 SN 0736-6205 J9 BIOTECHNIQUES JI Biotechniques PD MAR PY 1991 VL 10 IS 3 BP 332 EP 333 PG 2 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FB926 UT WOS:A1991FB92600012 PM 2064771 ER PT J AU PINCUS, SH WEHRLY, K CHESEBRO, B AF PINCUS, SH WEHRLY, K CHESEBRO, B TI USE OF A FOCAL INFECTIVITY ASSAY FOR TESTING SUSCEPTIBILITY OF HIV TO ANTIVIRAL AGENTS SO BIOTECHNIQUES LA English DT Article ID RICIN-A-CHAIN; SYNERGISTIC INHIBITION; INTERFERON-ALPHA; IMMUNODEFICIENCY; ZIDOVUDINE; INVITRO AB A highly sensitive and quantitative focal immunoassay has been developed for detecting the human immunodeficiency virus (HIV). The assay can be used to measure cell-free virus or the production of HIV by virus-infected cells. Both laboratory-adapted strains of HIV and patient isolates can be studied with this assay. In this communication, we demonstrate the utility of this assay for measuring the effects of anti-HIV agents on viral isolates. We show that the anti-viral effects of such diverse agents as azidothymidine, interferon-alpha, immunotoxins, soluble CD4 and antibody can be accurately quantified. This assay may be used in the discovery and evaluation of new anti-HIV therapies or may be adapted for use in testing the sensitivity of patient isolates to standard therapeutic agents. RP PINCUS, SH (reprint author), NIAID,ROCKY MT LABS,MICROBIAL STRUCT & FUNCT LAB,HAMILTON,MT 59840, USA. NR 12 TC 22 Z9 22 U1 0 U2 0 PU EATON PUBLISHING CO PI NATICK PA 154 E. CENTRAL ST, NATICK, MA 01760 SN 0736-6205 J9 BIOTECHNIQUES JI Biotechniques PD MAR PY 1991 VL 10 IS 3 BP 336 EP 342 PG 7 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FB926 UT WOS:A1991FB92600014 PM 2064772 ER PT J AU NERI, A BARRIGA, F INGHIRAMI, G KNOWLES, DM NEEQUAYE, J MAGRATH, IT DALLAFAVERA, R AF NERI, A BARRIGA, F INGHIRAMI, G KNOWLES, DM NEEQUAYE, J MAGRATH, IT DALLAFAVERA, R TI EPSTEIN-BARR-VIRUS INFECTION PRECEDES CLONAL EXPANSION IN BURKITTS AND ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED LYMPHOMA SO BLOOD LA English DT Article ID B-CELL LYMPHOPROLIFERATION; VIRAL-DNA; SYNDROME AIDS; DISORDERS; DISEASES; TERMINI C1 COLUMBIA UNIV COLL PHYS & SURG,DEPT PATHOL,NEW YORK,NY 10032. COLUMBIA UNIV COLL PHYS & SURG,CTR CANC,NEW YORK,NY 10032. NCI,PEDIAT BRANCH,BETHESDA,MD 20892. UNIV ACCRA,SCH MED,ACCRA,GHANA. OI neri, antonino/0000-0001-9047-5912 FU NCI NIH HHS [CA-37295, CA48236] NR 24 TC 158 Z9 161 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD MAR 1 PY 1991 VL 77 IS 5 BP 1092 EP 1095 PG 4 WC Hematology SC Hematology GA EZ330 UT WOS:A1991EZ33000025 PM 1847310 ER PT J AU HILAKIVICLARKE, LA TURKKA, J LISTER, RG LINNOILA, M AF HILAKIVICLARKE, LA TURKKA, J LISTER, RG LINNOILA, M TI EFFECTS OF EARLY POSTNATAL HANDLING ON BRAIN BETA-ADRENOCEPTORS AND BEHAVIOR IN TESTS RELATED TO STRESS SO BRAIN RESEARCH LA English DT Article DE HANDLING; ISOLATION; PORSOLTS SWIM TEST; STRESS; ALCOHOL INTAKE; ANXIETY; AGGRESSION; BETA-ADRENOCEPTOR; RAT ID FORCED SWIMMING TEST; ANTIDEPRESSANT ACTIVITY; ETHANOL-CONSUMPTION; EARLY EXPERIENCE; RAT-BRAIN; PHARMACOLOGY; STIMULATION; DEPRESSION; RECEPTORS; DESPAIR AB The present study investigated the effects of early postnatal handling and temporary maternal isolation, between the 5th and 20th postnatal days, on various behaviors related to stress measured in adulthood in male Wistar rats. In addition, beta-adrenoceptor binding in the brain was measured. The handling consisted of daily 3-min sessions during which a pup was gently held by an investigator. The isolated rat pups were kept separated from their nursing mothers for 1 h daily. Subsequently, it was found that the time spent immobile in Porsolt's swim test was shortened, and voluntary alcohol (5% v/v) consumption was reduced in the handled rats, as compared with the non-handled and isolated animals. No differences in the measure of anxiety - food consumed in a novel environment - or the time spent in social, aggressive and defensive behaviors in a resident-intruder paradigm, were noted. Neither did the density or affinity of beta-adrenoceptors in the frontal cortex or hippocampus differ significantly between the groups. The results indicate that short-lasting maternal separation does not cause sustained effects on behavior in the rat. Early postnatal handling leads to shortened immobility in the swim test and reduced voluntary alcohol consumption, suggesting that handled rats show an improved ability to cope with stress. RP HILAKIVICLARKE, LA (reprint author), NIAAA,CLIN STUDIES LAB,DICBR,BLDG 10,ROOM 3C102,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 43 TC 101 Z9 102 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD MAR 1 PY 1991 VL 542 IS 2 BP 286 EP 292 DI 10.1016/0006-8993(91)91580-T PG 7 WC Neurosciences SC Neurosciences & Neurology GA FB829 UT WOS:A1991FB82900017 PM 1851458 ER PT J AU LINET, MS DEVESA, SS AF LINET, MS DEVESA, SS TI DESCRIPTIVE EPIDEMIOLOGY OF CHILDHOOD LEUKEMIA SO BRITISH JOURNAL OF CANCER LA English DT Article ID ACUTE-LEUKEMIA; UNITED-STATES; CANCER; TRENDS; CHILDREN; BRITAIN; WHITES AB Internationally there is a 4-fold variation in age-adjusted incidence rates for childhood leukaemia (all types combined), with only slightly greater worldwide differences specifically for acute lymphocytic leukaemia (ALL) and for acute nonlymphocytic leukaemia (ANLL). Total leukaemia rates are highest among Hispanic populations in Costa Rica and Los Angeles (males), due primarily to elevated ALL incidence, while low rates occur among US blacks, Kuwaitis, Israeli non-Jews, and Bombay Indians. In most populations the patterns for ALL are similar to those for total leukaema, with peak incidence at ages 1-4 and a decline thereafter. Lower and more uniform rates are generally observed at all ages for ANLL. Age-adjusted rates for ANLL appear to vary substantially among some populations with uniform ALL incidence rates (e.g., among Asians) and yet appear to be similar in other populations with variation in ALL rates (e.g., whites and blacks in the US). Possible variation among registries in completeness of childhood leukaemia ascertainment and accuracy of diagnosis by cell type should be assessed, while case-control investigations among populations with very high and very low rates may provide useful information about the cell-type specific determinants of childhood leukaemia. RP LINET, MS (reprint author), NCI,DIV CANC ETIOL,EPIDEMIOL & BIOSTAT PROGRAM,BIOSTAT BRANCH,BETHESDA,MD 20892, USA. NR 42 TC 65 Z9 72 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0007-0920 J9 BRIT J CANCER JI Br. J. Cancer PD MAR PY 1991 VL 63 IS 3 BP 424 EP 429 DI 10.1038/bjc.1991.98 PG 6 WC Oncology SC Oncology GA FC082 UT WOS:A1991FC08200022 PM 2003985 ER PT J AU SLOAND, EM SLOAND, JA PRODOUZ, K KLEIN, HG YU, MW HARVATH, L FRICKE, W AF SLOAND, EM SLOAND, JA PRODOUZ, K KLEIN, HG YU, MW HARVATH, L FRICKE, W TI REDUCTION OF PLATELET GLYCOPROTEIN IB IN UREMIA SO BRITISH JOURNAL OF HAEMATOLOGY LA English DT Article ID RENAL-FAILURE; VONWILLEBRAND-FACTOR; FACTOR-VIII; SURFACE GLYCOPROTEINS; POLYACRYLAMIDE GELS; CHRONIC UREMIA; HEMODIALYSIS; PROTEINS; PLASMA; CRYOPRECIPITATE AB Patients with uraemia have abnormal platelet function that may be partially corrected by haemodialysis, cryoprecipitate or 1-desamino-8-D-arginine vasopressin (DDAVP). We studied the platelet von Willebrand factor receptor, glycoprotein Ib (GPIb), and plasma von Willebrand factor (vWF) in uraemic patients undergoing chronic haemodialysis. Using the slope of agglutination of formalin-fixed platelets as an index of response to ristocetin (with a constant amount of normal plasma as a source of vWF), we found the response of platelets from uraemic patients, both before (2.7 +/- 1.5, n = 40) and after dialysis (1.2 +/- 1.2, n = 40) to be significantly less than that for normal controls (14.1 +/- 10.2, n = 20; P < 0.001). In addition, the agglutination response of platelets obtained after dialysis was less than that of platelets obtained before dialysis (P < 0.001). Immunoblotting demonstrated decreased or absent staining of glycocalicin, a subunit of GPIb, in platelet lysates from 25 patients. All platelet samples with reduced glycocalicin also had decreased responses to ristocetin. Tritium-labelled platelets from seven patients showed decreased labelling of a protein with an electrophoretic mobility equivalent to that of GPIb (140 000 daltons). In addition, platelets with the lowest levels of surface GPIb, as demonstrated by flow cytometry, also had decreased ristocetin agglutination and decreased staining on immunoblot. Levels of von Willebrand factor antigen and ristocetin cofactor in plasma from 10 patients were generally within the normal range, although post-dialysis levels tended to be higher than pre-dialysis levels. The pre- and post-dialysis plasma vWF multimeric patterns were normal. C1 US FDA,CTR BIOL EVALUAT & RES,MARYLAND,MD 20892. NIH,DEPT TRANFUS MED,BETHESDA,MD 20892. UNIV ROCHESTER,SCH MED & DENT,ROCHESTER,NY 14620. NR 33 TC 51 Z9 51 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0007-1048 J9 BRIT J HAEMATOL JI Br. J. Haematol. PD MAR PY 1991 VL 77 IS 3 BP 375 EP 381 DI 10.1111/j.1365-2141.1991.tb08587.x PG 7 WC Hematology SC Hematology GA FA808 UT WOS:A1991FA80800020 PM 2012763 ER PT J AU HIRSCHFELD, RMA AF HIRSCHFELD, RMA TI DEPRESSIVE-ILLNESS - DIAGNOSTIC ISSUES SO BULLETIN OF THE MENNINGER CLINIC LA English DT Article ID CHARACTEROLOGICAL DEPRESSIONS; DISORDERS; CRITERIA C1 NIMH,MOOD ANXIETY & PERSONAL DISORDERS RES BRANCH,BETHESDA,MD 20892. NR 19 TC 3 Z9 3 U1 1 U2 1 PU MENNINGER FOUNDATION PI TOPEKA PA BOX 829, TOPEKA, KS 66601 SN 0025-9284 J9 B MENNINGER CLIN JI Bull. Menninger Clin. PD SPR PY 1991 VL 55 IS 2 BP 144 EP 155 PG 12 WC Psychiatry; Psychology, Psychoanalysis SC Psychiatry; Psychology GA FK549 UT WOS:A1991FK54900002 PM 2043894 ER PT J AU GOLDMAN, DL POST, RM AF GOLDMAN, DL POST, RM TI THE PUZZLE OF NONCOMPLIANCE IN THE MANIC PATIENT SO BULLETIN OF THE MENNINGER CLINIC LA English DT Article ID LITHIUM TREATMENT; SEQUENCE; SAMPLE C1 CF MENNINGER MEM HOSP,TOPEKA,KS 66601. NIMH,BIOL PSYCHIAT BRANCH,BETHESDA,MD 20892. RP GOLDMAN, DL (reprint author), A BARTON HEPBURN HOSP,214 KING ST,OGDENSBURG,NY 13669, USA. NR 11 TC 3 Z9 3 U1 0 U2 0 PU MENNINGER FOUNDATION PI TOPEKA PA BOX 829, TOPEKA, KS 66601 SN 0025-9284 J9 B MENNINGER CLIN JI Bull. Menninger Clin. PD SPR PY 1991 VL 55 IS 2 BP 248 EP 253 PG 6 WC Psychiatry; Psychology, Psychoanalysis SC Psychiatry; Psychology GA FK549 UT WOS:A1991FK54900010 PM 2043901 ER PT J AU BURKE, E LI, FP JANOV, AJ BATTER, S GRIER, H GOORIN, A AF BURKE, E LI, FP JANOV, AJ BATTER, S GRIER, H GOORIN, A TI CANCER IN RELATIVES OF SURVIVORS OF CHILDHOOD SARCOMA SO CANCER LA English DT Article ID RISK AB Relatives of 88 long-term survivors of childhood sarcoma were examined for the familial cancer syndrome of sarcoma, breast cancer, and other neoplasms (Li-Fraumeni syndrome). Twenty-six of 402 close relatives developed cancer (expected, 23.8), including breast cancer in four mothers (expected, 3.1). Two sarcoma probands who developed second malignant tumors have multiple relatives with cancer and might have an inherited predisposition. An increased cancer risk and exceptional requirement for disease screening appear to be confined to first-degree relatives of a small fraction of children with sarcoma, notably probands with second cancer. C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DEPT BIOSTAT & EPIDEMIOL,44 BINNEY ST,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DEPT PEDIAT ONCOL,BOSTON,MA 02115. HARVARD UNIV,CHILDRENS HOSP,SCH MED,BOSTON,MA 02115. NCI,CLIN EPIDEMIOL BRANCH,CLIN STUDIES SECT,BETHESDA,MD 20892. NR 12 TC 13 Z9 13 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD MAR 1 PY 1991 VL 67 IS 5 BP 1467 EP 1469 DI 10.1002/1097-0142(19910301)67:5<1467::AID-CNCR2820670535>3.0.CO;2-4 PG 3 WC Oncology SC Oncology GA EX815 UT WOS:A1991EX81500034 PM 1991315 ER PT J AU SHEARS, SB AF SHEARS, SB TI STRUCTURAL AND FUNCTIONAL INTERRELATIONSHIP OF THE INTRACELLULAR RECEPTOR FOR INOSITOL (1,4,5) TRISPHOSPHATE SO CANCER CELLS-A MONTHLY REVIEW LA English DT Editorial Material ID CALCIUM; PHOSPHATES RP SHEARS, SB (reprint author), NIEHS,CELLULAR & MOLEC PHARMACOL LAB,INOSITOL LIPID SECT,RES TRIANGLE PK,NC 27709, USA. NR 12 TC 1 Z9 1 U1 0 U2 0 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 1042-2196 J9 CANCER CELL-MON REV PD MAR PY 1991 VL 3 IS 3 BP 97 EP 99 PG 3 WC Oncology; Medicine, Research & Experimental SC Oncology; Research & Experimental Medicine GA FD998 UT WOS:A1991FD99800004 PM 1647191 ER PT J AU KNUTSEN, T BIXENMAN, HA LAWCE, H MARTIN, PK AF KNUTSEN, T BIXENMAN, HA LAWCE, H MARTIN, PK TI CHROMOSOME ANALYSIS GUIDELINES PRELIMINARY-REPORT SO CANCER GENETICS AND CYTOGENETICS LA English DT Article C1 PALO VERDE LAB,CHANDLER,AZ. OREGON HLTH SCI UNIV,CLIN CYTOGENET LAB,PORTLAND,OR 97201. SW HIGH TECH MED PK,CYTOGENET LAB,PALO HTS,IL. RP KNUTSEN, T (reprint author), NCI,CYTOGENET ONCOL SECT,BLDG 10,ROOM 12N-226,BETHESDA,MD 20892, USA. NR 5 TC 7 Z9 7 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0165-4608 J9 CANCER GENET CYTOGEN JI Cancer Genet. Cytogenet. PD MAR PY 1991 VL 52 IS 1 BP 11 EP 17 DI 10.1016/0165-4608(91)90048-Y PG 7 WC Oncology; Genetics & Heredity SC Oncology; Genetics & Heredity GA FG953 UT WOS:A1991FG95300002 PM 2009505 ER PT J AU SMITH, ICP SMART, CR FELLER, W BUNNAG, W MIYA, F HUGHES, P GILLETT, J HENSON, DE ANSELMINO, LM MARCUS, S MONCK, M STELMACK, G KROFT, T SAUNDERS, JK FOSSEL, ET AF SMITH, ICP SMART, CR FELLER, W BUNNAG, W MIYA, F HUGHES, P GILLETT, J HENSON, DE ANSELMINO, LM MARCUS, S MONCK, M STELMACK, G KROFT, T SAUNDERS, JK FOSSEL, ET TI A CLINICAL-TRIAL ON THE USE OF MAGNETIC-RESONANCE SPECTROSCOPY OF PLASMA FOR DETECTION OF CANCER SO CANCER JOURNAL LA English DT Article DE MAGNETIC RESONANCE SPECTROSCOPY; NMR; PLASMA CANCER; TRIGLYCERIDE; SCREENING; CLINICAL TRIAL ID HYPERTRIGLYCERIDEMIA; TUMORS; MICE AB We have investigated the plasma of a cohort of 925 individuals by water-suppressed proton nuclear magnetic resonance spectroscopy according to the procedure reported by Fossel et al. (New Engl J Med 1986; 315:1369-1377) to be capable of separating healthy individuals, and individuals ill with conditions other than cancer, from individuals with cancer. In addition, we have analyzed the plasma of these patients for triglycerides, cholesterol, and the lipoproteins LDL and HDL. H-1 NMR spectra were obtained at 360 MHz, 20 +/- 1-degrees-C, and analyzed, according to the methods of Fossel et al. Biochemical analyses were performed instrumentally using current laboratory methods. A large number of false positive results were obtained, which correlated in most cases with elevated levels of serum triglycerides in the control group. The NMR linewidth parameter showed an inverse nonlinear relationship with triglycerides levels, Figure 1. Overall, the untreated cancer patients, the cancer patients with no residual disease, and members of cancer-prone families were not significantly different from the control group with respect to linewidth parameter, plasma triglycerides, cholesterol, LDL and HDL. The patients treated for cancer but with residual disease showed significantly lower NMR linewidth parameters, higher triglyceride concentrations, and lower HDL levels. Within this group, abnormalities were most pronounced in individuals with metastatic disease. The large number of false tests indicates that the NMR linewidt parameter is not suitable for the screening of an asymptomatic population for cancer. C1 UNIV UTAH,SALT LAKE CITY,UT 84112. NCI,BETHESDA,MD 20892. COTTONWOOD HOSP,SALT LAKE CITY,UT. ABBOTT LABS,N CHICAGO,IL 60064. LATTER DAY ST HOSP,SALT LAKE CITY,UT 84143. GEORGETOWN UNIV,MED CTR,DEPT SURG,WASHINGTON,DC 20007. BETH ISRAEL HOSP,BOSTON,MA 02215. RP SMITH, ICP (reprint author), NATL RES COUNCIL CANADA,INST BIOL SCI,OTTAWA K1A 0R6,ONTARIO,CANADA. NR 20 TC 13 Z9 13 U1 0 U2 4 PU ASSOC DEVELOPPEMENT COMMUNICATION CANCEROLOGIQUE PI VILLEJUIF PA CANCER JOURNAL, 7 RUE GUY MOQUET, BP 8, 94801 VILLEJUIF, FRANCE SN 0765-7846 J9 CANCER J JI Cancer J. PD MAR-APR PY 1991 VL 4 IS 2 BP 125 EP 130 PG 6 WC Oncology SC Oncology GA FJ472 UT WOS:A1991FJ47200016 ER PT J AU LUCIER, GW TRITSCHER, A GOLDSWORTHY, T FOLEY, J CLARK, G GOLDSTEIN, J MARONPOT, R AF LUCIER, GW TRITSCHER, A GOLDSWORTHY, T FOLEY, J CLARK, G GOLDSTEIN, J MARONPOT, R TI OVARIAN HORMONES ENHANCE 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN-MEDIATED INCREASES IN CELL-PROLIFERATION AND PRENEOPLASTIC FOCI IN A 2-STAGE MODEL FOR RAT HEPATOCARCINOGENESIS SO CANCER RESEARCH LA English DT Article ID EPIDERMAL GROWTH-FACTOR; ESTROGEN-RECEPTOR; LIVER MICROSOMES; DOSE-RESPONSE; POSITIVE FOCI; BINDING; TCDD; DNA; DIETHYLNITROSAMINE; CYTOCHROME-P-450 AB 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a potent hepatocarcinogen in rodents. However, liver tumor incidence is increased by TCDD in female Sprague-Dawley rats but not male rats in chronic carcinogen bioassays. Our studies have investigated this finding by evaluating histological and biochemical parameters in a two-stage model for hepatocarcinogenesis in female Sprague-Dawley rats (intact and ovariectomized), using diethylnitrosamine (DEN) as the initiating agent and TCDD as the promoting agent. Increases in gamma-glutamyl transpeptidase-positive foci were greater in intact female rats than in ovariectomized (OVX) animals. For example, in intact rats receiving both DEN and TCDD, the percentage of liver occupied by gamma-glutamyl transpeptidase-positive foci was 0.37, compared to 0.08 in OVX rats. Values for intact or OVX rats receiving either DEN or TCDD only were 0.04 or less. Similar results were obtained when using placental glutathione S-transferase to detect hepatic preneoplastic lesions. Cell proliferation data, obtained using bromodeoxyuridine in osmotic minipumps, were consistent with preneoplastic foci data in that the hepatocyte labeling index was increased in DEN/TCDD intact rats but not in DEN/TCDD OVX rats. Analysis of data from individual animals revealed a strong correlation (P < 0.01) between cell proliferation and placental glutathione S-transferase-positive foci/cm3 in liver. These findings did not reflect effects of ovariectomy on TCDD tissue distribution, since livers of OVX rats contained more TCDD than livers of intact rats, although both groups of rats received a dose of 1.4-mu-g TCDD/kg once every 2 weeks for 30 weeks. Hepatic cytochrome P-450d (IA2) was induced approximately 6-8-fold in all TCDD-treated groups, and the magnitude of induction was not influenced by ovariectomy. This cytochrome efficiently catalyzes metabolism of 17-beta-estradiol to catechol estrogens. Our data suggest that ovarian hormones (probably estrogens) play a significant role in the hepatocarcinogenic actions of TCDD. C1 CHEM IND INST TOXICOL,RES TRIANGLE PK,NC 27709. RP LUCIER, GW (reprint author), NIEHS,MD A3-02,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 51 TC 147 Z9 148 U1 0 U2 3 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 1 PY 1991 VL 51 IS 5 BP 1391 EP 1397 PG 7 WC Oncology SC Oncology GA EZ618 UT WOS:A1991EZ61800007 PM 1671757 ER PT J AU DIGIOVANNI, J WALKER, SC BELTRAN, L NAITO, M EASTIN, WC AF DIGIOVANNI, J WALKER, SC BELTRAN, L NAITO, M EASTIN, WC TI EVIDENCE FOR A COMMON GENETIC PATHWAY CONTROLLING SUSCEPTIBILITY TO MOUSE SKIN TUMOR PROMOTION BY DIVERSE CLASSES OF PROMOTING AGENTS SO CANCER RESEARCH LA English DT Article ID PROTEIN KINASE-C; ORNITHINE DECARBOXYLASE INDUCTION; MICE FOLLOWING EXPOSURE; SENCAR MICE; BENZOYL PEROXIDE; INITIATING ACTIVITY; HYDROGEN-PEROXIDE; EPIDERMAL-CELLS; PHORBOL ESTERS; FEMALE SENCAR AB The present study has compared different mouse stocks and strains with known sensitivity to phorbol ester skin tumor promotion for their sensitivities to skin tumor promotion by a prototypic organic peroxide (benzoyl peroxide, BzPo) and anthrone (chrysarobin, Chr) tumor promoter. Following initiation with either 7,12-dimethylbenz(a)anthracene and/or N-methyl-N'-nitro-N-nitrosoguanidine, groups of mice were promoted with several different doses of each promoting agent. Among mice selectively bred for sensitivity to phorbol ester promotion, the order of sensitivity to BzPo was inbred SENCAR (SSIn) > SENCAR > CD-1. With Chr as the promoter, the order of sensitivity was SENCAR > SSIn > CD-1. Concurrent tumor promotion experiments examined the responsiveness of two common inbred mouse strains, DBA/2 and C57BL/6. The phorbol ester-responsive mouse strain, DBA/2, was more sensitive to skin tumor promotion by Chr than was C57BL/6 at all doses tested but was clearly less sensitive than both SENCAR and SSIn mice. Finally, DBA/2 and C57BL/6 mice were similar in their responsiveness to BzPo promotion, but again both of these inbred strains were significantly less sensitive than were SSIn and SENCAR mice to this organic peroxide type of skin tumor promoter. Histological evaluations comparing SENCAR and C57BL/6 mice revealed that a major difference between these strains in response to multiple Chr and BzPo treatments was in the inflammatory response (measured by edema formation). Unlike 12-O-tetradecanoylphorbol-13-acetate, Chr and BzPo did not induce dramatic differences in the epidermal hyperplasia (as measured by epidermal thickness) in these two mouse lines. The results presented in this paper suggest that there is a common pathway controlling susceptibility to skin tumor promotion by 12-O-tetradecanoylphorbol-13-acetate, BzPo, and chrysarobin. These results are discussed in terms of a possible genetic model(s) for skin tumor promotion in mice. C1 NIEHS,CARCINOGENESIS & TOXICOL EVALUAT BRANCH,RES TRIANGLE PK,NC 27709. RP DIGIOVANNI, J (reprint author), UNIV TEXAS,MD ANDERSON CANC CTR,DIV SCI PK RES,POB 389,SMITHVILLE,TX 78957, USA. FU NCI NIH HHS [CA 38871] NR 40 TC 35 Z9 35 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 1 PY 1991 VL 51 IS 5 BP 1398 EP 1405 PG 8 WC Oncology SC Oncology GA EZ618 UT WOS:A1991EZ61800008 PM 1900038 ER PT J AU CHAUHAN, SS GOLDSTEIN, LJ GOTTESMAN, MM AF CHAUHAN, SS GOLDSTEIN, LJ GOTTESMAN, MM TI EXPRESSION OF CATHEPSIN-L IN HUMAN TUMORS SO CANCER RESEARCH LA English DT Article ID MAJOR EXCRETED PROTEIN; MURINE FIBROBLASTS; TRANSFORMATION; CYSTEINE; CANCER; CELLS; CDNA; MEP; SECRETION; SEQUENCE AB It has been proposed that proteases secreted by cancer cells facilitate tumor invasion and metastasis by degrading the components of extracellular membranes. The lysosomal cysteine protease cathepsin L is synthesized in large amounts and secreted by many malignantly transformed cells in culture. The secreted protease is potent in degrading collagen, laminin, elastin, and other structural proteins of basement membranes. To determine whether human cancers synthesize cathespin L, the expression of cathespin L in approximately 100 human tumor samples was determined by quantitative RNA slot blot analysis using a specific human cathepsin L complementary DNA probe. Results of the present study suggest that cancers in general express higher levels of cathepsin L than do normal tissues. Kidney and testicular tumors expressed the highest levels of cathepsin L; non-small cell carcinomas of the lung expressed the next highest levels; and most cancers of the breast, ovary, colon, adrenal, bladder, prostate, and thyroid expressed elevated levels as well. Cathepsin L may prove useful as a diagnostic or prognostic marker of human malignancy. C1 NCI,CELL BIOL LAB,BLDG 37,ROOM 1B22,BETHESDA,MD 20892. NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 33 TC 175 Z9 180 U1 0 U2 5 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 1 PY 1991 VL 51 IS 5 BP 1478 EP 1481 PG 4 WC Oncology SC Oncology GA EZ618 UT WOS:A1991EZ61800020 PM 1997186 ER PT J AU DANFORTH, DN SGAGIAS, MK AF DANFORTH, DN SGAGIAS, MK TI INTERLEUKIN-1 ALPHA BLOCKS ESTRADIOL-STIMULATED GROWTH AND DOWN-REGULATES THE ESTROGEN-RECEPTOR IN MCF-7 BREAST-CANCER CELLS-INVITRO SO CANCER RESEARCH LA English DT Article ID MESSENGER RIBONUCLEIC-ACID; PROTEIN-LEVELS; PROLIFERATION; LINES; INCREASES; CULTURE; BINDING; RNA; DNA AB We studied the effect of interleukin 1-alpha (IL-1-alpha) on estradiol stimulation of cell growth and estrogen receptor (ER) content in MCF-7 human breast cancer cells in vitro to determine if IL-1-alpha altered cellular estradiol responsiveness. We found that IL-1-alpha blocked estradiol-stimulated growth of these cells in a dose-dependent manner (complete antagonism at 1000 units/ml: day 7 mean growth = vehicle, 47.7-mu-g DNA; estradiol 10(-10) M, 95.1; IL-1-alpha/estradiol, 44.6) and at all concentrations of estradiol from 10(-8) to 10(-11) M. IL-1-alpha in combination with trans-hydroxytamoxifen further inhibited estradiol-stimulated growth (vehicle = 44.8-mu-g DNA, estradiol = 108.3, estradiol/trans-hydroxytamoxifen = 47.8, IL-1-alpha/estradiol/trans-hydroxytamoxifen = 3.0, P < 0.01). Inhibition with trans-hydroxytamoxifen was IL-1-alpha dose dependent (maximum = 97% at 1000 units/ml, P < 0.01) and estradiol dose dependent (reversible with 10(-8) M estradiol, maximum inhibition at 10(-10) M estradiol). Concomitantly, IL-1-alpha down-regulated ER concentration by 38.0-43.7% (P < 0.01) as measured by immunoreactivity or Scatchard analysis, respectively. This occurred as early as 3 h without a change in the K(d) (vehicle = 0.23 nM, IL-1-alpha = 0.24 nM), persisted for at least 48 h, was dose dependent (maximum, 43.7% at 1000 units/ml, P < 0.01), and was blocked by cycloheximide. IL-1-alpha, did not block estradiol stimulation of progesterone receptor content (vehicle = 221.9, IL-1-alpha = 238.9 fmol/mg protein) and did not block estradiol down-regulation of ER content. Furthermore, IL-1-alpha alone did not alter levels of ER mRNA and did not alter estradiol down-regulation of ER mRNA. These findings indicate that while IL-1-alpha antagonizes estradiol stimulation of growth and reduces ER content, its mechanism may involve other non-estrogen-regulated pathways. RP DANFORTH, DN (reprint author), NCI,SURG BRANCH,BLDG 10,RM 2B38,BETHESDA,MD 20892, USA. NR 32 TC 41 Z9 41 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 1 PY 1991 VL 51 IS 5 BP 1488 EP 1493 PG 6 WC Oncology SC Oncology GA EZ618 UT WOS:A1991EZ61800022 PM 1997187 ER PT J AU PRESTI, JC RAO, PH CHEN, Q REUTER, VE LI, FP FAIR, WR JHANWAR, SC AF PRESTI, JC RAO, PH CHEN, Q REUTER, VE LI, FP FAIR, WR JHANWAR, SC TI HISTOPATHOLOGICAL, CYTOGENETIC, AND MOLECULAR CHARACTERIZATION OF RENAL CORTICAL TUMORS SO CANCER RESEARCH LA English DT Article ID GROWTH-FACTOR RECEPTORS; CELL-CARCINOMA; CHROMOSOME-7; PAPILLARY; DELETION; CANCER; HETEROZYGOSITY; ABNORMALITIES; EXPRESSION AB We used cytogenetic and restriction fragment length polymorphism (RFLP) analysis methods to define genetic alterations and also correlate the changes with histopathology in renal cortical tumors. The study series is comprised of 50 renal tumors in 4 histological categories: (a) clear cell, nonpapillary, renal cell carcinoma (RCC) (n = 32); (b) nonclear cell, nonpapillary RCC (n = 10); (c) papillary RCC (n = 3); and (d) oncocytic tumors (n = 5). Successful karyotypes were obtained from 28 tumors (56%), of which 17 (61%) were abnormal. Abnormalities of chromosome 3p were seen in 9 tumors, which included unbalanced translocations and terminal or interstitial deletions. Abnormalities of chromosome 5 were identified in 11 tumors, 8 of which were due to unbalanced translocations between 3p and 5q, resulting in an extra copy for the region 5q22-->ter. In addition, trisomy or tetrasomy of chromosome 17 was seen in 6 (5 with normal chromosome 3 and one with 3p deletion), trisomy or more copies of chromosome 7 in 8 (4 with 3p deletion, 2 with trisomy or tetrasomy 17, and 2 with trisomy alone), and trisomy 12 in 3 (all with trisomy 17) tumors. Furthermore, relative deficiency of chromosome 17p was seen in 3 (all with deletion 3p) and chromosome 18 in 4 (all with deletion 3p) tumors. RFLP analysis with four chromosome 3 specific probes detected 3p deletions in 19 tumors with the most common breakpoint located between 3p14-21. The 19 3p deletions detected by RFLP included tumors that also showed rearrangement of 3p by cytogenetics (n = 14) and those that showed normal karyotypes (n = 3) in addition to cytogenetic failures (n = 12). Deletions of 17p were seen in 5 of 31 informative cases. Thus, deletions of 3p were seen in a total of 24 tumors by cytogenetic and/or RFLP analysis, 21 of which were clear cell, nonpapillary RCC, whereas 3 had a minor clear-cell component. Oncocytic and nonclear, nonpapillary tumors, on the other hand, did not demonstrate 3p deletions by either technique, whereas trisomy 17 was seen in 3 of the 3 papillary tumors. The loss of alleles from chromosome 17p and 18 and an increased dosage of gene or genes on chromosomes 5q and 7 as seen in high-stage tumors of various histological subtypes may be associated with progression of disease. C1 MEM SLOAN KETTERING CANC CTR,DEPT PATHOL,CYTOGENET SERV,1275 YORK AVE,NEW YORK,NY 10021. MEM SLOAN KETTERING CANC CTR,DEPT SURG & CYTOGENET,DIV UROL,NEW YORK,NY 10021. NCI,CLIN EPIDEMIOL BRANCH,BOSTON,MA 02115. FU NCI NIH HHS [N01-CP-71126, CA-09207-11] NR 37 TC 193 Z9 194 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 1 PY 1991 VL 51 IS 5 BP 1544 EP 1552 PG 9 WC Oncology SC Oncology GA EZ618 UT WOS:A1991EZ61800031 PM 1671759 ER PT J AU VICKERS, AEM LUCIER, GW AF VICKERS, AEM LUCIER, GW TI ESTROGEN-RECEPTOR, EPIDERMAL GROWTH-FACTOR RECEPTOR AND CELLULAR PLOIDY IN ELUTRIATED SUBPOPULATIONS OF HEPATOCYTES DURING LIVER-TUMOR PROMOTION BY 17-ALPHA-ETHINYLESTRADIOL IN RATS SO CARCINOGENESIS LA English DT Article ID DRUG-METABOLIZING-ENZYMES; ORAL-CONTRACEPTIVES; ETHINYL ESTRADIOL; HEPATOCELLULAR-CARCINOMA; CENTRIFUGAL ELUTRIATION; N-NITROSOMORPHOLINE; HEPATIC NEOPLASIA; MAMMALIAN LIVER; FEMALE RATS; HEPATOCARCINOGENESIS AB 17-alpha-Ethinylestradiol (EE2)-mediated promotion of diethylnitrosamine (DEN)-initiated liver tumors was evaluated in distinct hepatocyte subpopulations. Our initiation - promotion regime consisted of a single dose of DEN (200 mg/kg) to ovariectomized rats, followed by chronic exposure to EE2 (90-mu-g/kg/day for 30 weeks). We observed significant increases in liver and uterine organ wts which were associated with liver tumor formation. Isolated hepatocytes were separated by elutriation into seven subpopulations. The early eluting subpopulations consisted of a greater proportion of diploid cells and they exhibited a preferential uptake of acridine orange, which is characteristic of periportal cells. With the increasing order of elutriated fractions, hepatocyte subpopulations of tetraploid and octaploid cells were obtained. Elutriation revealed that EE2 promotion enhanced nuclear estrogen receptor levels (3-fold) and gamma-glutamyltranspeptidase activity (5-fold) to a greater extent in the early eluting diploid subpopulations (1 and 2), even though total estrogen receptor (ER) levels were higher in the later eluting subpopulations. The stimulatory effect of EE2 on ER levels was associated with an increased ER occupancy in all subpopulations, although the effect was greatest in the later eluting fractions. Chronic EE2 exposure induced the emergence of new hepatocyte populations within fractions 6 and 7. Enhanced cell growth was observed in the DEN/EE2-derived hepatocytes by flow cytometric measurements of DNA synthesis. The new populations of altered cells expressed high levels of epidermal growth factor receptor (EGFR), with 90% of the cells positive for EGFR-antibody. In summary, our data demonstrate that many effects of EE2 on hepatocyte pathways involved in growth control occur in nearly all populations of cells, derived by elutriation although some effects such as the emergence of an EGFR-enriched population of hepatocytes are localized in specific populations. C1 NIEHS,POB 12233,RES TRIANGLE PK,NC 27709. NR 54 TC 27 Z9 27 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD MAR PY 1991 VL 12 IS 3 BP 391 EP 399 DI 10.1093/carcin/12.3.391 PG 9 WC Oncology SC Oncology GA FD350 UT WOS:A1991FD35000004 PM 1672626 ER PT J AU ONEILL, L HOLBROOK, NJ FARGNOLI, J LAKATTA, EG AF ONEILL, L HOLBROOK, NJ FARGNOLI, J LAKATTA, EG TI PROGRESSIVE CHANGES FROM YOUNG-ADULT AGE TO SENESCENCE IN MESSENGER-RNA FOR RAT CARDIAC MYOSIN HEAVY-CHAIN GENES SO CARDIOSCIENCE LA English DT Article DE SENESCENT HEART; CARDIAC MYOSIN HEAVY CHAIN MESSENGER RNA ID PRESSURE-OVERLOAD; ATPASE ACTIVITY; CONTRACTION DURATION; HYPERTROPHY; HEART; HYPERTENSION; PERFORMANCE; EXPRESSION; MYOCARDIUM; MYOCYTES AB In rodents of an advanced age the cardiac contraction exhibits a reduced shortening velocity and prolonged time course. This is, in part, due to a reduction in myosin ATPase activity which results from a shift in the myosin heavy chains from the alpha (V1) to the beta (V3) isoform. The present study demonstrates that during adult aging the steady state levels of alpha myosin heavy chain mRNA decrease while those of beta myosin heavy chain increase; for both mRNAs the respective changes are greater than fourfold. Thus, the phenotypic, biophysical and biochemical cardiac contractile changes with adult aging are, at least, in part regulated at the level of gene expression. C1 NIA,MOLEC GENET LAB,4940 EASTERN AVE,BALTIMORE,MD 21224. NIA,CARDIOVASC SCI LAB,BALTIMORE,MD 21224. NR 25 TC 61 Z9 61 U1 0 U2 1 PU CANAL PRESS PI VENICE PA S POLO 2171, 30125 VENICE, ITALY SN 1015-5007 J9 CARDIOSCIENCE JI Cardioscience PD MAR PY 1991 VL 2 IS 1 BP 1 EP 5 PG 5 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA FC088 UT WOS:A1991FC08800002 PM 1888877 ER PT J AU QIAN, SW KONDAIAH, P CASSCELLS, W ROBERTS, AB SPORN, MB AF QIAN, SW KONDAIAH, P CASSCELLS, W ROBERTS, AB SPORN, MB TI A 2ND MESSENGER-RNA SPECIES OF TRANSFORMING GROWTH FACTOR-BETA-1 IN INFARCTED RAT-HEART SO CELL REGULATION LA English DT Article ID FACTOR-BETA PRECURSOR; EXPRESSION; COLLAGEN; CLONING; PROMOTER; FIBROSIS; SEQUENCE; INVITRO; TISSUES; INVIVO AB Transforming growth factor-beta-1 (TGF-beta-1) is encoded predominantly by a 2.4-kb mRNA in most tissues. However, an additional transcript of 1.9 kb can be detected in rat heart after experimental myocardial infraction caused by ligation of the left coronary artery. This transcript level is significantly higher in infarcted heart tissue than in normal heart tissue, suggesting an important role for this mRNA species in response to injury. Structural characterization of the 1.9-kb mRNA showed that it included the entire coding sequence present in the 2.4-kb TGF-beta-1 mRNA, but also contained an additional nonhomologous 3'-untranslated region (UTR). The junction between the shared and unique 3' sequence in the 1.9-kb mRNA occurred only two nucleotides before the proposed polyadenylation site of the rat TGF-beta-1 2.4-kb mRNA. The unique 3'-UTR and the deduced shortened 5'-UTR in the novel 1.9-kb TGF-beta-1 mRNA suggest different transcriptional and translational regulatory mechanisms under conditions of tissue injury. C1 NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. RP QIAN, SW (reprint author), NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892, USA. NR 30 TC 52 Z9 52 U1 0 U2 0 PU AMER SOC CELL BIOL PI BETHESDA PA PUBL OFFICE 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 1044-2030 J9 CELL REGUL PD MAR PY 1991 VL 2 IS 3 BP 241 EP 249 PG 9 WC Cell Biology SC Cell Biology GA FF114 UT WOS:A1991FF11400007 PM 1859854 ER PT J AU BONNER, JC BRODY, AR AF BONNER, JC BRODY, AR TI ASBESTOS-INDUCED ALVEOLAR INJURY - EVIDENCE FOR MACROPHAGE-DERIVED PDGF AS A MEDIATOR OF THE FIBROGENIC RESPONSE SO CHEST LA English DT Article; Proceedings Paper CT 33RD ANNUAL THOMAS L PETTY ASPEN LUNG CONF : MECHANISMS OF LUNG REPAIR CY JUN 06-09, 1990 CL GIVEN INST PATHOBIOL, ASPEN, CO SP FRANCIS FAMILIES FDN, ALLEN & HANBURYS, BOEHRINGER INGELHEIM, FISONS, GENETECH, RORER PHARM, SCHERING, UPJOHN, ASPEN LUNG CONF ENDOWMENT FUND HO GIVEN INST PATHOBIOL ID RATS RP BONNER, JC (reprint author), NIEHS,PULM PATHOBIOL LAB,BLDG 104,S CAMPUS,RES TRIANGLE PK,NC 27709, USA. NR 10 TC 11 Z9 11 U1 0 U2 0 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD MAR PY 1991 VL 99 IS 3 SU S BP S54 EP S55 DI 10.1378/chest.99.3_Supplement.54S PG 2 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA FB305 UT WOS:A1991FB30500028 PM 1997275 ER PT J AU BRODY, AR BONNER, JC AF BRODY, AR BONNER, JC TI PLATELET-DERIVED GROWTH-FACTOR PRODUCED BY PULMONARY CELLS SO CHEST LA English DT Article; Proceedings Paper CT 33RD ANNUAL THOMAS L PETTY ASPEN LUNG CONF : MECHANISMS OF LUNG REPAIR CY JUN 06-09, 1990 CL GIVEN INST PATHOBIOL, ASPEN, CO SP FRANCIS FAMILIES FDN, ALLEN & HANBURYS, BOEHRINGER INGELHEIM, FISONS, GENETECH, RORER PHARM, SCHERING, UPJOHN, ASPEN LUNG CONF ENDOWMENT FUND HO GIVEN INST PATHOBIOL ID ALVEOLAR MACROPHAGES; STIMULATES PROLIFERATION; INVITRO; EXPRESSION; RELEASE; SECRETION; MOLECULE; ASBESTOS; BINDING; REPAIR RP BRODY, AR (reprint author), NIEHS,PULM PATHOBIOL LAB,BLDG 104,S CAMPUS,RES TRIANGLE PK,NC 27709, USA. NR 38 TC 9 Z9 9 U1 0 U2 0 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD MAR PY 1991 VL 99 IS 3 SU S BP S50 EP S52 DI 10.1378/chest.99.3_Supplement.50S PG 3 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA FB305 UT WOS:A1991FB30500026 PM 1997273 ER PT J AU FRIEDMAN, BS METCALFE, DD AF FRIEDMAN, BS METCALFE, DD TI EFFECTS OF TIXOCORTOL PIVALATE ON GASTROINTESTINAL-DISEASE IN SYSTEMIC MASTOCYTOSIS - A PRELIMINARY-STUDY SO CLINICAL AND EXPERIMENTAL ALLERGY LA English DT Article ID CIMETIDINE AB Tixocortol pivalate is a steroid reportedly without significant adrenal-pituitary axis suppression when administered via the gastrointestinal tract. To determine whether this steroid would suppress the gastrointestinal manifestations of systemic mastocytosis, we performed an open clinical trial for safety and efficacy with tixocortol pivalate in four patients for periods of 8-15 weeks. All patients showed a decrease in the symptoms of abdominal pain and frequency of stools. Laboratory parameters of malabsorption improved in parallel with symptom relief. Histopathologic abnormalities of the small bowel improved in one patient. There was no significant suppression of the pituitary-adrenal axis. Two patients developed fluid retention while on tixocortol pivalate, which was attributed to a mineralocorticoid effect. One patient had a fall in AM cortisol. In summary, this study strongly suggests that tixocortol pivalate, when administered orally, has gastrointestinal anti-inflammatory activity comparable to conventional steroids, but may not be entirely without adrenal-suppressive effect and may lead to fluid retention in some patients. Further studies are warranted to assess the value of tixocortol pivalate in the therapy of inflammatory diseases of the upper gastrointestinal tract. C1 NIAID,MAST CELL PHYSIOL SECT,CLIN INVEST LAB,BLDG 10,ROOM 11C210,BETHESDA,MD 20892. NR 15 TC 9 Z9 9 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0954-7894 J9 CLIN EXP ALLERGY JI Clin. Exp. Allergy PD MAR PY 1991 VL 21 IS 2 BP 183 EP 188 DI 10.1111/j.1365-2222.1991.tb00828.x PG 6 WC Allergy; Immunology SC Allergy; Immunology GA FB077 UT WOS:A1991FB07700006 PM 2043986 ER PT J AU CASH, JM KLIPPEL, JH AF CASH, JM KLIPPEL, JH TI RHEUMATIC DISEASES AND CANCER SO CLINICAL AND EXPERIMENTAL RHEUMATOLOGY LA English DT Editorial Material ID SYSTEMIC LUPUS-ERYTHEMATOSUS; POLYMYOSITIS-DERMATOMYOSITIS; B-CELLS; INTRACEREBRAL LYMPHOMA; MALIGNANT NEOPLASMS; LONG-TERM; ARTHRITIS; CYCLOPHOSPHAMIDE; FREQUENCY; MORTALITY RP CASH, JM (reprint author), NIAMS,BLDG 10,ROOM 9N-240,BETHESDA,MD 20892, USA. NR 37 TC 18 Z9 18 U1 0 U2 0 PU CLINICAL & EXPER RHEUMATOLOGY PI PISA PA VIA SANTA MARIA 31, 56126 PISA, ITALY SN 0392-856X J9 CLIN EXP RHEUMATOL JI Clin. Exp. Rheumatol. PD MAR-APR PY 1991 VL 9 IS 2 BP 109 EP 112 PG 4 WC Rheumatology SC Rheumatology GA FH351 UT WOS:A1991FH35100001 PM 2060156 ER PT J AU BALLIN, M MACKAY, AR HARTZLER, JL NASON, A PELINA, MD THORGEIRSSON, UP AF BALLIN, M MACKAY, AR HARTZLER, JL NASON, A PELINA, MD THORGEIRSSON, UP TI RAS LEVELS AND METALLOPROTEINASE ACTIVITY IN NORMAL VERSUS NEOPLASTIC RAT MAMMARY TISSUES SO CLINICAL & EXPERIMENTAL METASTASIS LA English DT Article ID NIH 3T3 CELLS; C-HA-RAS; METASTATIC PHENOTYPE; ONCOGENE ACTIVATION; CARCINOMA-CELLS; NIH/3T3 CELLS; IV COLLAGEN; NUDE-MICE; EXPRESSION; CANCER AB We have previously reported that activated ras oncogenes can simultaneously switch on the metastatic phenotype and increased capability to degrade type IV collagen [36]. Here the relationship between c-H-ras, metalloproteinase expression and metastatic behavior was studied in N-nitrosomethylurea (NMU)-induced rat mammary carcinomas, which are known to possess activated c-H-ras. When comparing normal rat breast tissue to mammary carcinomas there was no direct relationship between ras DNA levels and neoplastic changes. Furthermore, there were no consistent differences between metastatic and non-metastatic carcinomas, or between primary tumors and metastases. The NMU-induced rat mammary carcinomas expressed two major gelatinolytic metal-loproteinases (gelatinases) of 65 and 92 kD, but only the 65 kD gelatinase was detected in normal breast tissue and a rat fibroma. Type IV collagenolytic activity per 5-mu-g of protein was two to three times higher in the mammary carcinomas than in the normal breasts, whereas the primary tumors did not differ from the corresponding metastases. This study shows that ras amplification is not necessary for development of the malignant or metastatic phenotype in the NMU-induced rat mammary carcinoma model. We have also found that induction of p21 ras protein synthesis in a v-H-ras transfected NIH/3T3 (433) cell line, containing a glucocorticoid promoter, does not lead to an increase in metastatic capacity. C1 CATHOLIC UNIV AMER,DEPT CELL BIOL,WASHINGTON,DC 20064. RP BALLIN, M (reprint author), NCI,DIV CANC ETIOL,OFF DIRECTOR,BETHESDA,MD 20892, USA. OI Mackay, Andrew Reay/0000-0001-7096-3759 NR 41 TC 22 Z9 22 U1 0 U2 2 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0262-0898 J9 CLIN EXP METASTAS JI Clin. Exp. Metastasis PD MAR-APR PY 1991 VL 9 IS 2 BP 179 EP 189 DI 10.1007/BF01756388 PG 11 WC Oncology SC Oncology GA FP964 UT WOS:A1991FP96400008 PM 2032422 ER PT J AU HOFFMAN, T LEE, YL LIZZIO, EF TRIPATHI, AK JESSOP, JJ TAPLITS, M ABRAHAMSEN, TG CARTER, CS PURI, J AF HOFFMAN, T LEE, YL LIZZIO, EF TRIPATHI, AK JESSOP, JJ TAPLITS, M ABRAHAMSEN, TG CARTER, CS PURI, J TI ABSENCE OF MODULATION OF MONOKINE PRODUCTION VIA ENDOGENOUS CYCLOOXYGENASE OR 5-LIPOXYGENASE METABOLITES - MK-886 (3-[1-(4-CHLOROBENZYL)-3-TERT-BUTYL-THIO-5-ISOPROPYLINDOL-2-YL]-2,2-DIME THYLPROPANOIC ACID), INDOMETHACIN, OR ARACHIDONATE FAIL TO ALTER IMMUNOREACTIVE INTERLEUKIN-1-BETA, OR TNF-ALPHA PRODUCTION BY HUMAN MONOCYTES INVITRO SO CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article ID TUMOR NECROSIS FACTOR; IL-1 PRODUCTION; MONONUCLEAR PHAGOCYTES; PROSTAGLANDINS; PHOSPHOLIPASE; LEUKOTRIENES C1 NCI,DIV CANC THERAPY,PEDIAT ONCOL BRANCH,BETHESDA,MD 20892. NIH,CTR CLIN,DEPT TRANSFUS MED,BETHESDA,MD 20892. WEIZMANN INST SCI,DEPT CHEM IMMUNOL,IL-76100 REHOVOT,ISRAEL. RP HOFFMAN, T (reprint author), US FDA,CTR BIOL EVALUAT & RES,DIV HEMATOL,CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 21 TC 20 Z9 20 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0090-1229 J9 CLIN IMMUNOL IMMUNOP JI Clin. Immunol. Immunopathol. PD MAR PY 1991 VL 58 IS 3 BP 399 EP 408 DI 10.1016/0090-1229(91)90130-3 PG 10 WC Immunology; Pathology SC Immunology; Pathology GA EY830 UT WOS:A1991EY83000009 PM 1900463 ER PT J AU FUDALA, PJ HEISHMAN, SJ HENNINGFIELD, JE JOHNSON, RE AF FUDALA, PJ HEISHMAN, SJ HENNINGFIELD, JE JOHNSON, RE TI HUMAN PHARMACOLOGY AND ABUSE POTENTIAL OF NALMEFENE SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Article ID OPIOID ANTAGONIST; NALTREXONE; SAFETY; KINETICS; EFFICACY; MORPHINE; BINDING; BRAIN AB Nalmefene hydrochloride was administered to six male volunteers with histories of opitate abuse using a double-blind, randomized, Latin square design to determine if it produced typical morphine-like effects. A comparison of physiologic and subject- and observer-reported effects was made between morphine, 15 and 30 mg given intramuscularly; nalmefene, 25, 50, and 100 mg given orally; and placebo. Drowsiness or sleepiness was the most common drug effect reported after the administration of each treatment. Only morphine produced miosis and increased subject-reported euphoria and "drug liking." Neither drug increased Addiction Research Inventory subscale scores measuring dysphoria or sedation or produced changes on the Profile of Mood States questionnaire. Adverse effects reported only after the administration of nalmefene included agitation/irritability and muscle tension; these did not appear to be dose related. The data indicated that nalmefene did not produce typical morphine-like effects and has no apparent abuse potential. RP FUDALA, PJ (reprint author), NIDA,ADDICT RES CTR,FRANCIS SCOTT KEY MED CTR,TREATMENT BRANCH,POB 5180,BALTIMORE,MD 21224, USA. NR 24 TC 23 Z9 25 U1 1 U2 2 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD MAR PY 1991 VL 49 IS 3 BP 300 EP 306 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FE195 UT WOS:A1991FE19500012 PM 2007323 ER PT J AU OREN, DA SHANNON, NJ CARPENTER, CJ ROSENTHAL, NE AF OREN, DA SHANNON, NJ CARPENTER, CJ ROSENTHAL, NE TI USAGE PATTERNS OF PHOTOTHERAPY IN SEASONAL AFFECTIVE-DISORDER SO COMPREHENSIVE PSYCHIATRY LA English DT Article ID LIGHT; ANTIDEPRESSANT RP OREN, DA (reprint author), NIMH,CLIN PSYCHOBIOL BRANCH,BLDG 10,ROOM 45239,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 16 TC 31 Z9 31 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0010-440X J9 COMPR PSYCHIAT JI Compr. Psychiat. PD MAR-APR PY 1991 VL 32 IS 2 BP 147 EP 152 DI 10.1016/0010-440X(91)90007-Y PG 6 WC Psychiatry SC Psychiatry GA FA954 UT WOS:A1991FA95400007 PM 2022114 ER PT J AU IHDE, DC MINNA, JD AF IHDE, DC MINNA, JD TI NON-SMALL-CELL LUNG-CANCER .1. BIOLOGY, DIAGNOSIS, AND STAGING SO CURRENT PROBLEMS IN CANCER LA English DT Article ID BRONCHIAL NEEDLE ASPIRATION; L-MYC GENE; LONG-TERM SURVIVORS; K-RAS ONCOGENE; RETINOBLASTOMA SUSCEPTIBILITY GENE; FLEXIBLE FIBEROPTIC BRONCHOSCOPY; PREOPERATIVE ASSESSMENT; RESPIRATORY CANCER; POINT MUTATIONS; CHANGING HISTOPATHOLOGY AB Squamous, large cell, and adenocarcinoma, collectively termed non-small cell lung cancer (NSCLC), are diagnosed in approximately 75% of patients with lung cancer in the United States. The treatment of these three tumor cell types is approached in virtually identical fashion because, in contrast to small cell carcinoma of the lung, NSCLC more frequently presents with localized disease at the time of diagnosis and is thus more often amenable to surgical resection but less frequently responds to chemotherapy and irradiation. Cigarette smoking is etiologically related to the development of NSCLC in the great majority of cases. Genetic mutations in dominant oncogenes such as K-ras, loss of genetic material on chromosomes 3p, 11p, and 17p, and deletions or mutations in tumor suppressor genes such as rb and p53 have been documented in NSCLC tumors and tumor cell lines. NSCLC is diagnosed because of symptoms related to the primary tumor or regional or distant metastases, as an incidental finding on chest radiograph, or rarely because of a paraneoplastic syndrome such as hypercalcemia or hypertrophic pulmonary osteoarthropathy. Screening smokers with periodic chest radiographs and sputum cytologic examination has not been shown to reduce mortality. The diagnosis of NSCLC is usually established by fiberoptic bronchoscopy or percutaneous fine-needle aspiration, by biopsy of a regional or distant metastatic site, or at the time of thoracotomy. Pathologically, NSCLC arises in a setting of bronchial mucosal metaplasia and dysplasia that progressively increase over time. Squamous carcinoma more often presents as a central endobronchial lesion, while large cell and adenocarcinoma have a tendency to arise in the lung periphery and invade the pleura. Once the diagnosis is made, the extent of tumor dissemination is determined. Since most NSCLC patients who survive 5 years or longer have undergone surgical resection of their cancers, the focus of the staging process is to determine whether the patient is a candidate for thoracotomy with curative intent. The dominant prognostic factors in NSCLC are extent of tumor dissemination, ambulatory or performance status, and degree of weight loss. Stages I and II NSCLC, which are confined within the pleural reflection, are managed by surgical resection whenever possible, with approximate 5-year survival of 45% and 25%, respectively. Patients with stage IIIa cancers, in which the primary tumor has extended through the pleura or metastasized to ipsilateral or subcarinal lymph nodes, can occasionally be surgically resected but are often managed with definitive thoracic irradiation and have 5-year survival of approximately 15%. In completely surgically resected NSCLC, postoperative irradiation reduces the local recurrence rate but has not been shown to improve survival. Stage IIIb cancer, which is more advanced than stage IIIa but without distant metastases, is frequently treated with chest irradiation; 5-year survival is less than 5%. Thoracic radiotherapy can cure a small fraction of stage III NSCLC patients and perhaps up to 15% to 20% of stages I and II patients with medical contraindications to surgery. Although higher doses of irradiation up to 6,000 cGy with conventional fractionation are associated with improved response rates and better local control, survival is not improved, almost certainly because of the predominant effects of distant metastases in determining eventual outcome. Patients with stage IV NSCLC, in which distant metastases are present at diagnosis, have only anecdotal 5-year survival. In the past decade, however, some evidence of biologic effects of cisplatin-based combination chemotherapy has been documented. Although chemotherapy cannot be said to unequivocally improve survival in any stage of NSCLC, some prospective randomized trials have shown survival or disease-free survival advantages in patients with overt stage IV disease or as an adjuvant treatment before or after definitive irradiation or surgery. Other such trials have been negative, however, and continued clinical trials will be required before a role for chemotherapy in NSCLC can be considered to be established. At present, outside the setting of a clinical trial, patients should receive chemotherapy only if they are categorically incurable by surgery or irradiation, have no significant symptoms that could be readily palliated with irradiation, are fully ambulatory, have assessable tumor lesions so that treatment can be discontinued if ineffective, and desire therapy after being informed of its limitations. C1 NCI, NAVY MED ONCOL BRANCH, BETHESDA, MD 20892 USA. NATL NAVAL MED CTR, BETHESDA, MD 20814 USA. RP IHDE, DC (reprint author), UNIFORMED SERV UNIV HLTH SCI, BETHESDA, MD 20814 USA. NR 222 TC 88 Z9 89 U1 1 U2 14 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0147-0272 J9 CURR PROB CANCER JI Curr. Probl. Cancer PD MAR-APR PY 1991 VL 15 IS 2 BP 61 EP + PG 0 WC Oncology SC Oncology GA FG609 UT WOS:A1991FG60900001 PM 1649734 ER PT J AU ALLEN, JB BANSAL, GP FELDMAN, GM HAND, AO WAHL, LM WAHL, SM AF ALLEN, JB BANSAL, GP FELDMAN, GM HAND, AO WAHL, LM WAHL, SM TI SUPPRESSION OF BACTERIAL-CELL WALL INDUCED POLYARTHRITIS BY RECOMBINANT GAMMA INTERFERON SO CYTOKINE LA English DT Article DE ARACHIDONIC ACID METABOLITES; ARTHRITIS; INTERFERONS ID ARACHIDONIC-ACID METABOLISM; INHIBITS COLLAGEN-SYNTHESIS; TUMOR NECROSIS FACTOR; SYSTEMIC INJECTION; FIBROBLAST PROLIFERATION; RHEUMATOID-ARTHRITIS; CANCER-PATIENTS; IFN-GAMMA; RATS; MACROPHAGES C1 NIDR,IMMUNOL LAB,CELLULAR IMMUNOL SECT,BETHESDA,MD 20892. NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892. NR 43 TC 16 Z9 16 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4666 J9 CYTOKINE JI Cytokine PD MAR PY 1991 VL 3 IS 2 BP 98 EP 106 DI 10.1016/1043-4666(91)90029-D PG 9 WC Biochemistry & Molecular Biology; Cell Biology; Immunology SC Biochemistry & Molecular Biology; Cell Biology; Immunology GA FZ153 UT WOS:A1991FZ15300003 PM 1909589 ER PT J AU WITKIN, JM JOHNSON, RE JAFFE, JH GOLDBERG, SR GRAYSON, NA RICE, KC KATZ, JL AF WITKIN, JM JOHNSON, RE JAFFE, JH GOLDBERG, SR GRAYSON, NA RICE, KC KATZ, JL TI THE PARTIAL OPIOID AGONIST, BUPRENORPHINE, PROTECTS AGAINST LETHAL EFFECTS OF COCAINE SO DRUG AND ALCOHOL DEPENDENCE LA English DT Article DE COCAINE; BUPRENORPHINE; MORPHINE; METHADONE; CONVULSIONS; LETHALITY; CXBK MICE ID CENTRAL NERVOUS-SYSTEM; OPIATE RECEPTORS; ABUSE; BINDING; AGENT; MICE; DETOXIFICATION; PHARMACOLOGY; MORPHINE; MOUSE AB Buprenorphine (0.3-3.0 mg/kg) produced dose-dependent protection against the lethal effects of cocaine in mice. The (+)-enantiomer of buprenorphine did not protect up to doses over 100 times greater than the lowest effective dose of its (-)-enantiomer. The protective effects were also produced by the opioid agonists morphine and methadone, but not by the opioid antagonist, naltrexone. Low doses of naltrexone (0.3-1.0 mg/kg) blocked the protective effects of buprenorphine. Protection conferred by buprenorphine was not observed in CXBK mice, a recombinant inbred strain relatively devoid of mu-opioid receptors. Thus, buprenorphine appears to protect against the lethal effects of cocaine by a process mediated by mu-opioid receptors. The present results should provide some additional safety assurance in future clinical trials with buprenorphine, especially in outpatient trials where cocaine abuse may continue along with treatment. C1 NIDDKD,MED CHEM LAB,BETHESDA,MD 20892. RP WITKIN, JM (reprint author), NIDA,ADDICT RES CTR,PSYCHOBIOL LAB,BOX 5180,BALTIMORE,MD 21224, USA. OI Katz, Jonathan/0000-0002-1068-1159 NR 33 TC 30 Z9 30 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0376-8716 J9 DRUG ALCOHOL DEPEN JI Drug Alcohol Depend. PD MAR PY 1991 VL 27 IS 2 BP 177 EP 184 DI 10.1016/0376-8716(91)90037-Y PG 8 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA FG354 UT WOS:A1991FG35400011 PM 1647294 ER PT J AU KNECHT, KT MASON, RP AF KNECHT, KT MASON, RP TI THE DETECTION OF HALOCARBON-DERIVED RADICAL ADDUCTS IN BILE AND LIVER OF RATS SO DRUG METABOLISM AND DISPOSITION LA English DT Article ID CARBON-TETRACHLORIDE METABOLISM; ISOLATED HEPATOCYTES; INVIVO; ACTIVATION; CCL4; BROMOTRICHLOROMETHANE; CHLOROFORM; MICROSOMES; HEPATOTOXICITY; PRODUCTS AB The free radical metabolism of halocarbons has been studied in living animals by the techniques of spin trapping and electron paramagnetic resonance. Earlier work demonstrated that radical adducts of carbon tetrachloride can be detected in the bile of living rats treated with carbon tetrachloride and the spin trap phenyl-N-t-butyl nitrone. In this study, the biles and livers of treated animals have been examined in order to determine the factors that could affect the content of radical adduct detected in bile (e.g. bile flow rate). The approaches used include the quantitation of radiolabeled spin trap and of the trichloromethyl radical adduct in bile and liver, and the pharmacological manipulation of bile flow. Other halogenated hydrocarbons are thought to form free radicals in vivo, and have also been studied by these techniques. Bromotrichloromethane, a brominated analog of carbon tetrachloride, readily forms the same radical adducts as carbon tetrachloride. No radical adduct from chloroform, the corresponding hydrogenated analog, is detectable in bile. Radical adduct is only detectable in bile from bromoform-treated rats after the production of hypoxia. C1 UNIV N CAROLINA,CHAPEL HILL,NC 27514. RP KNECHT, KT (reprint author), NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 26 TC 26 Z9 26 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0090-9556 J9 DRUG METAB DISPOS JI Drug Metab. Dispos. PD MAR-APR PY 1991 VL 19 IS 2 BP 325 EP 331 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FE239 UT WOS:A1991FE23900007 PM 1676631 ER PT J AU MAHMOOD, NA OVERSTREET, D BURKA, LT AF MAHMOOD, NA OVERSTREET, D BURKA, LT TI COMPARATIVE DISPOSITION AND METABOLISM OF 1,2,3-TRICHLOROPROPANE IN RATS AND MICE SO DRUG METABOLISM AND DISPOSITION LA English DT Article ID 1,2-DIBROMO-3-CHLOROPROPANE DBCP; TOXICITY AB 1,2,3-Trichloropropane (TCP) has been used as a solvent and degreasing agent and as an intermediate in pesticide manufacture. TCP is currently the subject of a National Toxicology Program chronic toxicity study. The present study is part of a larger effort to characterize the toxicity of TCP. Following acute oral exposure of male and female F344 rats (30 mg/kg) and male B6C3F1 mice (30 and 60 mg/kg), TCP was rapidly absorbed, metabolized, and excreted. The major route of excretion of TCP was in the urine. By 60 hr postdosing, rats had excreted 50% and mice 65% of the administered dose by this route. Exhalation as (CO2)-C-14 and excretion in the feces each accounted for 20% of the total dose in 60 hr in rats and 20 and 15%, respectively, in mice. No apparent sex-related differences were observed in the ability of the rats to excrete TCP-derived radioactivity. At 60 hr, TCP-derived radioactivity was most concentrated in the liver, kidney, and forestomach in both rats and male mice. Male mice eliminated TCP-derived radioactivity more rapidly than rats and lower concentrations of radioactivity were found in tissues 60 hr after dosing in mice. Two urinary metabolites were isolated and identified by NMR, mass spectroscopy, and comparison with synthetic standards, as N-acetyl- and S-(3-chloro-2-hydroxypropyl)cysteine. Analyses of the early urine (0-6 hr) showed this mercapturic acid to be the major metabolite in rat urine and was only a minor component in mouse urine. 2-(S-Glutathionyl)malonic acid was identified by NMR and mass spectrometry and by chemical synthesis as the major biliary metabolite in rats. Formation of these metabolites indicate that oxidation and glutathione conjugation play a major role in the biotransformation of TCP. C1 NIEHS,ENVIRONM TOXICOL BRANCH,POB 12233,RES TRIANGLE PK,NC 27709. NR 23 TC 16 Z9 17 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0090-9556 J9 DRUG METAB DISPOS JI Drug Metab. Dispos. PD MAR-APR PY 1991 VL 19 IS 2 BP 411 EP 418 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FE239 UT WOS:A1991FE23900022 PM 1676646 ER PT J AU HERR, DW SANDERS, JM MATTHEWS, HB AF HERR, DW SANDERS, JM MATTHEWS, HB TI BRAIN DISTRIBUTION AND FATE OF TRIS(2-CHLOROETHYL) PHOSPHATE IN FISCHER 344 RATS SO DRUG METABOLISM AND DISPOSITION LA English DT Article ID KAINIC ACID; BEHAVIOR AB Tris(2-chloroethyl) phosphate (TRCP) is a flame retardant that has a wide variety of industrial applications. In subchronic studies, oral administration of TRCP to rats and mice has been reported to produce dose-, sex-, and species-dependent lesions in the hippocampal brain region. The present investigation has examined the metabolism, elimination, and regional brain distribution of [C-14]TRCP in male and female rats. [C-14]TRCP was administered by gavage (0, 175, 350, or 700 mg/kg) and urine, feces, exhaled volatiles, CO2, and selected tissues were collected. Regional brain distribution of C-14 was determined 2 hr following single doses of TRCP to male and female rats, and 24 hr after a single dose and the last of 14 daily doses of TRCP to female rats. Results of these studies indicate that TRCP is readily absorbed from the gastrointestinal tract, distributed to all brain regions, and that metabolism and excretion are nearly complete in 72 hr. Most of the TRCP-derived radioactivity was excreted in urine (up to 85%), with feces, volatiles, and CO2 combined accounting for less than 10% of the dose. Predominant signs of toxicity associated with TRCP administration (350 and 700 mg/kg) were seizures within 2 hr of treatment, when most of the TRCP-derived radioactivity present in brain tissue was in the form of the parent compound. Traces of inextractable C-14 were detected at later times, but this material was not concentrated in brain relative to other tissues. The lack of hippocampal accumulation of C-14 in any experiment indicates that the lesion of this brain region is due to factor(s) other than bioaccumulation of TRCP-derived material by this part of the brain. C1 NIEHS,NATL TOXICOL PROGRAM,EXPTL TOXICOL BRANCH,POB 12233,RES TRIANGLE PK,NC 27709. NR 23 TC 8 Z9 8 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0090-9556 J9 DRUG METAB DISPOS JI Drug Metab. Dispos. PD MAR-APR PY 1991 VL 19 IS 2 BP 436 EP 442 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FE239 UT WOS:A1991FE23900026 PM 1676650 ER PT J AU BURKA, LT SANDERS, JM HERR, DW MATTHEWS, HB AF BURKA, LT SANDERS, JM HERR, DW MATTHEWS, HB TI METABOLISM OF TRIS(2-CHLOROETHYL) PHOSPHATE IN RATS AND MICE SO DRUG METABOLISM AND DISPOSITION LA English DT Article ID FLAME-RETARDANT AB Tris(2-chloroethyl) phosphate (TRCP), a flame retardant, produces a dose-, sex-, and species-dependent lesion in the hippocampal region of the brain following subchronic oral administration. This lesion is more common and more severe in female F344 rats than in male F344 rats, and is not observed in B6C3F1 mice. The present investigation of the metabolism of TRCP was designed to detect sex and species variations that might account for differences in toxicity. Elimination of TRCP-derived radioactivity was more rapid in mice, which excreted > 70% of an oral dose of 175 mg/kg in urine in 8 hr vs. approximately 40% for male or female rats. However, the metabolic profile of TRCP-derived radioactivity in urine was similar for both species. The major metabolite in female rat urine was identified as bis(2-chloroethyl) carboxymethyl phosphate. This metabolite co-chromatographed with the major metabolite found in both male rat and mouse urine. Two additional metabolites identified in female rat urine were bis(2-chloroethyl) hydrogen phosphate and the glucuronide of bis(2-chloroethyl) 2-hydroxyethyl phosphate. These metabolites also cochromatographed with metabolites found in male rat and mouse urine. TRCP metabolism in rats was not induced or inhibited by nine daily 175 mg/kg doses. Toxicity, as evidenced by seizures, was potentiated in male rats pretreated with inhibitors of aldehyde dehydrogenase. RP BURKA, LT (reprint author), NIEHS,NATL TOXICOL PROGRAM,EXPTL TOXICOL BRANCH,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 13 TC 17 Z9 17 U1 2 U2 11 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0090-9556 J9 DRUG METAB DISPOS JI Drug Metab. Dispos. PD MAR-APR PY 1991 VL 19 IS 2 BP 443 EP 447 PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FE239 UT WOS:A1991FE23900027 PM 1676651 ER PT J AU BROWN, JL SONODA, S UEDA, H SCOTT, MP WU, C AF BROWN, JL SONODA, S UEDA, H SCOTT, MP WU, C TI REPRESSION OF THE DROSOPHILA-FUSHI-TARAZU (FTZ) SEGMENTATION GENE SO EMBO JOURNAL LA English DT Article DE FTZ; PREBLASTODERM EMBRYO; REPRESSOR; SEGMENTATION; ZEBRA ELEMENT ID MAJOR DEVELOPMENTAL TRANSITION; EARLY XENOPUS-EMBRYOS; DIFFERENTIAL REGULATION; EXPRESSION; PROTEIN; EMBRYOGENESIS; PATTERNS; HAIRY; RNA; TRANSCRIPTION AB The striped expression of the Drosophila segmentation gene fushi tarazu in alternate parasegments of the early embryo is controlled by the 740 bp zebra element. Among multiple protein factors that bind to the zebra element, FTZ-F2 behaves as a transcriptional repressor of ftz. Point mutations in the zebra element which disrupt FTZ-F2 binding to DNA cause ectopic expression of zebra-lacZ activity in transformed embryos. The mutant constructs are expressed from the zygotic genome in preblastoderm embryos as early as the third nuclear division cycle. This unprecedented early transcription suggests that ftz requires active repression during initial nuclear division cycles, a novel type of embryonic gene regulation. A putative FTZ-F2 cDNA clone isolated by recognition site screening of an expression library was found to be identical in sequence with the zinc finger protein tramtrack (Harrison and Travers, 1990). C1 NCI,BIOCHEM LAB,BETHESDA,MD 20892. UNIV COLORADO,HOWARD HUGHES MED INST,BOULDER,CO 80309. UNIV COLORADO,DEPT MOLEC CELLULAR & DEV BIOL,BOULDER,CO 80309. RI UEDA, Hitoshi/B-1455-2011; Tang, Amy/L-3226-2016 OI Tang, Amy/0000-0002-5772-2878 NR 49 TC 80 Z9 81 U1 0 U2 3 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0261-4189 J9 EMBO J JI Embo J. PD MAR PY 1991 VL 10 IS 3 BP 665 EP 674 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FB413 UT WOS:A1991FB41300021 PM 2001679 ER PT J AU BOHLER, HCL TRACER, H MERRIAM, GR PETERSEN, SL AF BOHLER, HCL TRACER, H MERRIAM, GR PETERSEN, SL TI CHANGES IN PROOPIOMELANOCORTIN MESSENGER-RIBONUCLEIC-ACID LEVELS IN THE ROSTRAL PERIARCUATE REGION OF THE FEMALE RAT DURING THE ESTROUS-CYCLE SO ENDOCRINOLOGY LA English DT Article ID LUTEINIZING-HORMONE; BETA-ENDORPHIN; LH-SECRETION; PROGESTERONE SECRETION; PRO-OPIOMELANOCORTIN; INSITU HYBRIDIZATION; MENSTRUAL-CYCLE; GENE-EXPRESSION; HYPOTHALAMUS; ESTRADIOL AB GnRH synthesis and release are regulated by a number of neurotransmitter systems. Several studies have implicated the opiodergic system as one of the important modulators of GnRH. To obtain an index of the activity of beta-endorphinseceting neurons during the estrous cycle, we measured levels of proopiomelanocortin mRNA (POMC mRNA) in the periarcuate region at different cycle stages, using in situ hybridization. Ten female Sprague-Dawley rats (200-230 g) were killed at each of 11 times during the 4-day estrous cycle. Fresh frozen sections were made through the rostral arcuate nucleus and placed on gelatin-coated slides. A 48-base probe complementary to rat POMC mRNA was 3' end-labeled with [S-35]dATP and applied to individual sections in hybridization buffer. Sections were washed and exposed to film. Relative amounts of POMC mRNA were measured by obtaining optical densities with an image analyzer. POMC mRNA levels varied significantly. At proestrus, they were low just before the onset of the LH surge, followed by a sharp rise that afternoon. On the day of estrus, POMC mRNA remained elevated and then declined again on metestrus. A second but smaller rise was seen in the late afternoon of metestrus. This pattern of changes in POMC mRNA is consistent with an inhibitory effect of beta-endorphin on GnRH after the midcycle surge and in the postovulatory phase of the cycle, while low levels of POMC mRNA in the early afternoon of proestrus may permit the release of GnRH, which triggers the LH surge. The changes in POMC mRNA approximately parallel changes in progesterone in the cycle. C1 UNIV MISSOURI,DEPT ANAT & NEUROBIOL,COLUMBIA,MO 65212. RP BOHLER, HCL (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,BLDG 10,ROOM 10N262,BETHESDA,MD 20892, USA. NR 38 TC 60 Z9 60 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD MAR PY 1991 VL 128 IS 3 BP 1265 EP 1269 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FA598 UT WOS:A1991FA59800008 PM 1999146 ER PT J AU JACOBSON, MA BESCH, CL CHILD, C HAFNER, R MUTH, K DEYTON, L AF JACOBSON, MA BESCH, CL CHILD, C HAFNER, R MUTH, K DEYTON, L TI CLINICAL-PROGRAMS FOR CLINICAL RESEARCH ON AIDS - DESCRIPTION OF A RANDOMIZED PROSPECTIVE-STUDY OF CLINDAMYCIN VERSUS PYRIMETHAMINE FOR PREVENTION OF TOXOPLASMA-GONDII INFECTION SO EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES LA English DT Note ID ACQUIRED IMMUNODEFICIENCY SYNDROME; IMMUNE-DEFICIENCY SYNDROME; ENCEPHALITIS; THERAPY AB The risk of toxoplasmic encephalitis complicating AIDS appears largely limited to those HIV-infected patients with serologic evidence of past Toxoplasma gondii infection and low CD4 lymphocyte counts. The Community Programs for Clinical Research on AIDS has initiated a randomized, placebo-controlled trial to determine if clindamycin or pyrimethamine prophylactic regimens are effective and safe in preventing toxoplasmic encephalitis. C1 SAN FRANCISCO GEN HOSP,SAN FRANCISCO,CA 94110. TULANE UNIV,MED CTR,LOUISIANA COMMUNITY AIDS RES PROGRAM,NEW ORLEANS,LA 70118. NIAID,AIDS DIV,BETHESDA,MD 20892. NIAID,COMMUNITY PROGRAMS CLIN RES AIDS,BETHESDA,MD 20892. RP JACOBSON, MA (reprint author), SAN FRANCISCO COMMUNITY CONSORTIUM AIDS,WARD 84,BLDG 80,995 POTRERO,SAN FRANCISCO,CA 94110, USA. NR 15 TC 5 Z9 5 U1 0 U2 0 PU FRIEDR VIEWEG SOHN VERLAG GMBH PI WIESBADEN 1 PA PO BOX 5829, W-6200 WIESBADEN 1, GERMANY SN 0934-9723 J9 EUR J CLIN MICROBIOL JI Eur. J. Clin. Microbiol. Infect. Dis. PD MAR PY 1991 VL 10 IS 3 BP 195 EP 198 DI 10.1007/BF01964462 PG 4 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA FK308 UT WOS:A1991FK30800017 PM 1676364 ER PT J AU BLACK, JR FEINBERG, J MURPHY, RL FASS, RJ CAREY, J SATTLER, FR AF BLACK, JR FEINBERG, J MURPHY, RL FASS, RJ CAREY, J SATTLER, FR TI CLINDAMYCIN AND PRIMAQUINE AS PRIMARY-TREATMENT FOR MILD AND MODERATELY SEVERE PNEUMOCYSTIS-CARINII PNEUMONIA IN PATIENTS WITH AIDS SO EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES LA English DT Note AB The combination of clindamycin and primaquine was investigated as primary treatment for mild or moderately severe Pneumocystis carinii pneumonia (alveolar-arterial oxygen gradient < 40 torr) in 36 AIDS patients. All patients were treated with primaquine 30 mg (base) orally for 21 days. The first 22 patients were treated with clindamycin 900 mg i.v. q8h for the first 10 days, followed by clindamycin 450 mg q6h orally for 11 days. Twenty of these 22 patients (91 %) showed a marked clinical response by day 7. Four patients were later withdrawn from the study due to toxicity. An additional 14 patients were treated with an entirely oral regimen of clindamycin and primaquine. Thirteen of the 14 patients (93 %) showed a marked therapeutic response and 12 (86 %) successfully completed treatment. The combination of clindamycin and primaquine appears to be an effective and well-tolerated treatment for mild to moderately severe Pneumocystis carinii pneumonia in AIDS patients. C1 NIAID,DIV AIDS,TREATMENT RES PROGRAM,BETHESDA,MD 20892. NORTHWESTERN UNIV,SCH MED,CHICAGO,IL 60611. OHIO STATE UNIV,COLUMBUS,OH 43210. CASE WESTERN RESERVE UNIV,CLEVELAND,OH 44106. UNIV SO CALIF,LOS ANGELES,CA 90089. RP BLACK, JR (reprint author), INDIANA UNIV,SCH MED,1633 N CAPITOL 700,INDIANAPOLIS,IN 46202, USA. OI Murphy, Robert/0000-0003-3936-2052 NR 3 TC 47 Z9 47 U1 0 U2 1 PU FRIEDR VIEWEG SOHN VERLAG GMBH PI WIESBADEN 1 PA PO BOX 5829, W-6200 WIESBADEN 1, GERMANY SN 0934-9723 J9 EUR J CLIN MICROBIOL JI Eur. J. Clin. Microbiol. Infect. Dis. PD MAR PY 1991 VL 10 IS 3 BP 204 EP 207 DI 10.1007/BF01964465 PG 4 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA FK308 UT WOS:A1991FK30800020 PM 2060532 ER PT J AU LAUNER, LJ KARDJATI, S KUSIN, JA REED, GF AF LAUNER, LJ KARDJATI, S KUSIN, JA REED, GF TI PATTERNS OF VARIABILITY IN THE NUTRIENT INTAKE OF NUTRITIONALLY VULNERABLE PREGNANT-WOMEN SO EUROPEAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID DIETARY AB Intra/inter-individual variance ratios for energy, protein, fat and retinol-equivalent intake during the third trimester of pregnancy were calculated. Data were collected on 743 pregnant women from rural Indonesia between 1982 and 1984. Food intake of these women was directly weighed for three consecutive days per month during months 6-9 of pregnancy, yielding 7139 observations for analysis. Ratios were calculated separately for a single month of pregnancy and for the last trimester of pregnancy. The intra/inter-individual variance ratio for kilocalorie and protein intake was < 1.0 for a single month; it was > 1.0 for the trimester. This suggest that women varied their intake of kilocalories and protein more over the course of their pregnancy than they did during one month of their pregnancy. Both the monthly and trimester variance ratios for fat and retinol-equivalent intake were > 1.0. Our findings suggest that usual mean intake during the third trimester of pregnancy can be measured with relative precision with two replicates per individual. However, more measurements are needed for regression analyses: measurement error greatly attenuated the coefficients, even though data were collected by the direct weighing method. Thus the number of measurements per individual needed in a study of nutrition and pregnancy depends on the nutrient, the period of 'usual intake' that is of interest, as well as the analytical strategy. C1 NATL INST CHILD HLTH & HUMAN DEV,DIV PREVENT RES,BETHESDA,MD. UNIV AIRLANGGA,FAC MED,NUTR LAB,SURABAYA,INDONESIA. ROYAL TROP INST,AMSTERDAM,NETHERLANDS. ERASMUS UNIV,SCH MED,DEPT EPIDEMIOL & BIOSTAT,3000 DR ROTTERDAM,NETHERLANDS. NR 15 TC 10 Z9 11 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0954-3007 J9 EUR J CLIN NUTR JI Eur. J. Clin. Nutr. PD MAR PY 1991 VL 45 IS 3 BP 131 EP 138 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA FR277 UT WOS:A1991FR27700002 PM 2065636 ER PT J AU CLERICI, M VIA, CS LUCEY, DR ROILIDES, E PIZZO, PA SHEARER, GM AF CLERICI, M VIA, CS LUCEY, DR ROILIDES, E PIZZO, PA SHEARER, GM TI FUNCTIONAL DICHOTOMY OF CD4+ T-HELPER LYMPHOCYTES IN ASYMPTOMATIC HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION SO EUROPEAN JOURNAL OF IMMUNOLOGY LA English DT Article ID IMMUNE-DEFICIENCY SYNDROME; ENVELOPE PROTEIN; CELL; HIV; ABNORMALITIES; INHIBITION; COMPLEX; INVITRO; GP120; AIDS AB The majority of asymptomatic, human immune deficiency virus seropositive (HIV+) individuals exhibit a defect in CD4+ T helper cell (T(h)) function that is selective for responses to recall antigens, but not to HLA alloantigens. The CD4-dependent T(h) response to HLA alloantigens (Allo) can be mediated by two distinct T(h) pathways: self-restricted CD4+ T(h) that recognize allogeneic determinants processed and presented by autologous or self accessory or antigen-presenting cells (sAC); and allo-restricted, CD4+ T(h) that recognize allogeneic determinants directly on allogeneic accessory or antigen-presenting cells (aAC). In contrast, the T(h) response to recall antigens requires CD4+ T(h) and sAC and is therefore limited to the major histocompatibility complex (MHC) self-restricted pathway. Peripheral blood leukocytes from 56 asymptomatic HIV+ patients that exhibited a selective defect in CD4+ T(h) function were analyzed to determine whether the T(h) response to Allo was entirely functional, or whether one of the CD4-mediated components of the Allo T(h) response was also defective. By depletion of AC and/or CD8+ T(h) subsets (to analyze CD4+ T(h) function), we demonstrated that HIV+ patients who were selectively deficient in T(h) function to recall antigens were also unresponsive to Allo presented by autologous AC (HLA self-restricted T(h) pathway), but retained Allo T(h) activity presented by allogeneic AC (allo-restricted CD4+ T(h) pathway). These findings indicate that the CD4+ T(h) defect seen in the majority of asymptomatic, HIV+ individuals is not limited to recall antigens, but also extends to the component of the response to HLA alloantigens that involves the self-restricted, CD4+ T(h) pathway. Thus, the T(h) defect observed in asymptomatic, HIV+ patients does not involve a CD4+ T(h) defect per se, but is limited to the HLA self-restricted component of T(h) function. C1 NCI,DIV CANC BIOL,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4B-17,BETHESDA,MD 20892. NCI,CTR DIAG,BETHESDA,MD 20892. NCI,DIV CANC TREATMENT,PEDIAT BRANCH,INFECT DIS SECT,BETHESDA,MD 20892. UNIV MARYLAND,SCH MED,DEPT MED,DIV RHEUMATOL & CLIN IMMUNOL,BALTIMORE,MD 21201. LOCH RAVEN VET ADM MED CTR,BALTIMORE,MD. WILFORD HALL USAF MED CTR,HIV UNIT,LACKLAND AFB,TX 78236. NR 21 TC 34 Z9 34 U1 0 U2 1 PU VCH PUBLISHERS INC PI DEERFIELD BEACH PA 303 NW 12TH AVE, DEERFIELD BEACH, FL 33442-1788 SN 0014-2980 J9 EUR J IMMUNOL JI Eur. J. Immunol. PD MAR PY 1991 VL 21 IS 3 BP 665 EP 670 DI 10.1002/eji.1830210319 PG 6 WC Immunology SC Immunology GA FF293 UT WOS:A1991FF29300018 PM 1672645 ER PT J AU HAZZARD, DG SOBAN, J AF HAZZARD, DG SOBAN, J TI ADDENDUM TO - STUDIES OF AGING USING DEFINED RODENTS - A BIBLIOGRAPHY SO EXPERIMENTAL AGING RESEARCH LA English DT Bibliography RP HAZZARD, DG (reprint author), NIH,OFF RESOURCE DEV,BETHESDA,MD 20892, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU TAYLOR & FRANCIS PI BRISTOL PA 1900 FROST ROAD, SUITE 101, BRISTOL, PA 19007-1598 SN 0361-073X J9 EXP AGING RES JI Exp. Aging Res. PD SPR PY 1991 VL 17 IS 1 BP 53 EP 61 PG 9 WC Geriatrics & Gerontology; Psychology SC Geriatrics & Gerontology; Psychology GA GK790 UT WOS:A1991GK79000006 PM 1936101 ER PT J AU SATOH, H BARRETT, JC OSHIMURA, M AF SATOH, H BARRETT, JC OSHIMURA, M TI INTRODUCTION OF NEW GENETIC-MARKERS ON HUMAN-CHROMOSOMES SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID MICROCELL-MEDIATED TRANSFER; CELL-LINE; TUMORIGENIC EXPRESSION; REGIONAL LOCALIZATION; THYMIDINE KINASE; CHINESE-HAMSTER; MAMMALIAN-CELLS; MOUSE CELLS; STEM-CELLS; RESISTANCE C1 NIEHS,MOLEC CARCINOGENESIS LAB,POB 12233,RES TRIANGLE PK,NC 27709. KANAGAWA CANC CTR RES INST,CYTOGENET LAB,ASAHI KU,YOKOHAMA 241,JAPAN. NR 28 TC 1 Z9 1 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD MAR PY 1991 VL 193 IS 1 BP 5 EP 11 DI 10.1016/0014-4827(91)90531-X PG 7 WC Oncology; Cell Biology SC Oncology; Cell Biology GA EY872 UT WOS:A1991EY87200002 PM 1995301 ER PT J AU HOLTER, W RABSON, AB CORSICO, CD HOWARD, BH AF HOLTER, W RABSON, AB CORSICO, CD HOWARD, BH TI SEQUENCE-SPECIFIC TOXICITY OF TRANSFECTED RETROVIRAL DNA SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; LONG TERMINAL REPEAT; III HTLV-III; MAMMALIAN-CELLS; EXPRESSION; GENE; PROTEIN; TRANSCRIPTION; INFECTION; ACCUMULATION C1 NCI,DIV CANC BIOL & DIAG,MOLEC BIOL LAB,BETHESDA,MD 20892. NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. HOWARD HUGHES INST,HHMI NIH RES SCHOLARS PROGRAM,BETHESDA,MD. NR 33 TC 9 Z9 9 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD MAR PY 1991 VL 193 IS 1 BP 54 EP 58 DI 10.1016/0014-4827(91)90537-5 PG 5 WC Oncology; Cell Biology SC Oncology; Cell Biology GA EY872 UT WOS:A1991EY87200008 PM 1847335 ER PT J AU VOLLBERG, TM GEORGE, MD JETTEN, AM AF VOLLBERG, TM GEORGE, MD JETTEN, AM TI INDUCTION OF EXTRACELLULAR-MATRIX GENE-EXPRESSION IN NORMAL HUMAN KERATINOCYTES BY TRANSFORMING GROWTH-FACTOR-BETA IS ALTERED BY CELLULAR-DIFFERENTIATION SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID HUMAN EPIDERMAL-KERATINOCYTES; FACTOR-BETA; MESSENGER-RNA; EPITHELIAL-CELLS; TERMINAL DIFFERENTIATION; SQUAMOUS DIFFERENTIATION; CHOLESTEROL SULFATE; MOLECULAR-CLONING; HUMAN FIBRONECTIN; RETINOIC ACID C1 NIEHS,PULM PATHOBIOL LAB,CELL BIOL GRP,POB 12233,RES TRIANGLE PK,NC 27709. OI Jetten, Anton/0000-0003-0954-4445 NR 45 TC 57 Z9 57 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD MAR PY 1991 VL 193 IS 1 BP 93 EP 100 DI 10.1016/0014-4827(91)90542-3 PG 8 WC Oncology; Cell Biology SC Oncology; Cell Biology GA EY872 UT WOS:A1991EY87200013 PM 1995305 ER PT J AU PASH, J SMITHGALL, T BUSTIN, M AF PASH, J SMITHGALL, T BUSTIN, M TI CHROMOSOMAL PROTEIN HMG-14 IS OVEREXPRESSED IN DOWN-SYNDROME SO EXPERIMENTAL CELL RESEARCH LA English DT Note ID TRANSCRIBED GENES C1 NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. GEORGETOWN UNIV,DEPT PHARMACOL,ROCKVILLE,MD 20850. RI Bustin, Michael/G-6155-2015 NR 20 TC 9 Z9 9 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD MAR PY 1991 VL 193 IS 1 BP 232 EP 235 DI 10.1016/0014-4827(91)90562-9 PG 4 WC Oncology; Cell Biology SC Oncology; Cell Biology GA EY872 UT WOS:A1991EY87200033 PM 1825298 ER PT J AU BODINE, DM MCDONAGH, KT SEIDEL, NE NIENHUIS, AW AF BODINE, DM MCDONAGH, KT SEIDEL, NE NIENHUIS, AW TI SURVIVAL AND RETROVIRUS INFECTION OF MURINE HEMATOPOIETIC STEM-CELLS INVITRO - EFFECTS OF 5-FU AND METHOD OF INFECTION SO EXPERIMENTAL HEMATOLOGY LA English DT Article DE INTERLEUKIN-3; INTERLEUKIN-6; HEMATOPOIESIS ID HUMAN ADENOSINE-DEAMINASE; MEDIATED GENE-TRANSFER; BETA-GLOBIN GENE; LONG-TERM EXPRESSION; REPOPULATING ABILITY; MOUSE; MICE; PROGENITORS; THERAPY; CULTURE AB Gene replacement therapy for diseases of the hematopoietic system requires efficient gene transfer to pluripotent hematopoietic stem cells. We have systematically compared a number of protocols for retrovirus-mediated gene transfer into murine repopulating hematopoietic stem cells. Recipients of infected bone marrow cells were analyzed for the presence of the transduced provirus 4 months after transplantation. Our results show that 5-fluorouracil (5-FU) pretreatment of donor animals was required for efficient gene transfer and that 5-FU-treated bone marrow retained more repopulating activity in culture than untreated bone marrow. A comparison of retrovirus-mediated gene transfer by co-cultivation of bone marrow cells with retrovirus producer cells as opposed to gene transfer by culturing bone marrow cells in retrovirus-containing supernatant revealed that gene transfer by cocultivation was more efficient than supernatant infection. However, the repopulating ability of bone marrow cells cocultured with retrovirus producer cells was reduced compared to bone marrow cells cultured in virus-containing medium. RP BODINE, DM (reprint author), NHLBI,CLIN HEMATOL BRANCH,9000 ROCKVILLE PIKE,BLDG 10,ROOM 7C103,BETHESDA,MD 20892, USA. NR 33 TC 131 Z9 132 U1 0 U2 0 PU CARDEN JENNINGS PUBL CO LTD PI CHARLOTTESVILLE PA BLAKE CTR, STE 200, 1224 W MAIN ST, CHARLOTTESVILLE, VA 22903 SN 0301-472X J9 EXP HEMATOL JI Exp. Hematol. PD MAR PY 1991 VL 19 IS 3 BP 206 EP 212 PG 7 WC Hematology; Medicine, Research & Experimental SC Hematology; Research & Experimental Medicine GA EZ182 UT WOS:A1991EZ18200011 PM 1704844 ER PT J AU GILAD, GM GILAD, VH AF GILAD, GM GILAD, VH TI POLYAMINES CAN PROTECT AGAINST ISCHEMIA-INDUCED NERVE-CELL DEATH IN GERBIL FOREBRAIN SO EXPERIMENTAL NEUROLOGY LA English DT Article ID ORNITHINE DECARBOXYLASE ACTIVITY; REVERSIBLE CEREBRAL-ISCHEMIA; RAT SYMPATHETIC NEURONS; D-ASPARTATE RECEPTOR; AXONAL INJURY; AMINO-ACIDS; BRAIN; GLUTAMATE; SPERMINE; BINDING RP GILAD, GM (reprint author), NIMH,NEUROSCI CTR ST ELIZABETHS,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032, USA. NR 55 TC 93 Z9 93 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4886 J9 EXP NEUROL JI Exp. Neurol. PD MAR PY 1991 VL 111 IS 3 BP 349 EP 355 DI 10.1016/0014-4886(91)90102-I PG 7 WC Neurosciences SC Neurosciences & Neurology GA FA217 UT WOS:A1991FA21700010 PM 1999235 ER PT J AU GLICK, ID CLARKIN, JF HAAS, GL SPENCER, JH CHEN, CL AF GLICK, ID CLARKIN, JF HAAS, GL SPENCER, JH CHEN, CL TI A RANDOMIZED CLINICAL-TRIAL OF INPATIENT FAMILY INTERVENTION .6. MEDIATING VARIABLES AND OUTCOME SO FAMILY PROCESS LA English DT Article ID SCHIZOPHRENIA; AFTERCARE C1 NIMH,ROCKVILLE,MD 20857. NEW YORK HOSP,WESTCHESTER DIV,WHITE PLAINS,NY 10605. UNIV PITTSBURGH,PITTSBURGH,PA 15260. WESTERN PSYCHIAT INST & CLIN,PITTSBURGH,PA 15261. YESHIVA UNIV ALBERT EINSTEIN COLL MED,BRONX,NY 10461. RP GLICK, ID (reprint author), CORNELL UNIV,MED CTR,COLL MED,PAYNE WHITNEY CLIN,525 E 68TH ST,NEW YORK,NY 10021, USA. FU NCRR NIH HHS [RR-05396]; NIMH NIH HHS [MH-34466] NR 9 TC 31 Z9 31 U1 2 U2 2 PU FAMILY PROCESS INC PI VERNON PA PO BOX 460, VERNON, NJ 07462 SN 0014-7370 J9 FAM PROCESS JI Fam. Process PD MAR PY 1991 VL 30 IS 1 BP 85 EP 99 DI 10.1111/j.1545-5300.1991.00085.x PG 15 WC Psychology, Clinical; Family Studies SC Psychology; Family Studies GA FC733 UT WOS:A1991FC73300008 PM 2044754 ER PT J AU ALBRIGHT, JF OPPENHEIM, JJ AF ALBRIGHT, JF OPPENHEIM, JJ TI CONTRIBUTIONS OF BASIC IMMUNOLOGY TO HUMAN HEALTH SO FASEB JOURNAL LA English DT Article DE MONOCLONAL ANTIBODY; TUMOR NECROSIS FACTOR; HEMATOPOIETIC STEM CELL; CELL ADHESION MOLECULE; LYMPHOCYTE HOMING RECEPTOR; AUTOIMMUNITY; CHIMERIC INTERFERONS; TRANSFORMING GROWTH FACTOR; COLONY STIMULATING FACTOR AB The sixth symposium in the series "Contemporary Topics in Immunology" was held in New Orleans on June 3, 1990, at the joint meeting of The American Association of Immunologists and the American Society of Biochemistry and Molecular Biology. The symposium was sponsored jointly by The American Association of Immunologists, the Clinical Immunology Society, and the National Institute of Allergy and Infectious Diseases, and was titled "The Contributions of Basic Immunology to Human Health." Five speakers, whose research has clear relevance to the treatment and prevention of major human diseases, discussed topics of great current interest: hematopoietic stem cells, cell adhesion and lymphocyte homing; the complexities of autoimmunity and approaches to diverting or depressing autoaggressive immunity; structure and functions of the interferons and the construction of designer and chimeric interferons; the varied functions of transforming growth factors and molecular events that regulate the synthesis of TGF-beta; and the roles of cytokines in the expression of human immuno-deficiency virus and the prospects for controlling HIV infections by regulating selected cytokines. This symposium will be remembered for the exceptional clarity with which each speaker illustrated how fundamental knowledge in immunology fuels advances in the treatment and prevention of those human disorders that involve the immune system. C1 NCI,FREDERICK CANC RES FACIL,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21701. RP ALBRIGHT, JF (reprint author), NIAID,DIV ALLERGY IMMUNOL & TRANSPLANTAT,BASIC IMMUNOL BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 1 PY 1991 VL 5 IS 3 BP 265 EP 270 PG 6 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA FC556 UT WOS:A1991FC55600002 PM 1705905 ER PT J AU ALEXANDER, NJ AF ALEXANDER, NJ TI VASECTOMY AND HUMAN-IMMUNODEFICIENCY-VIRUS OF MICE AND MEN - REPLY SO FERTILITY AND STERILITY LA English DT Letter RP ALEXANDER, NJ (reprint author), NICHHD,CONTRACEPT DEV BRANCH,BETHESDA,MD 20892, USA. NR 2 TC 1 Z9 1 U1 0 U2 0 PU AMER SOC REPRODUCTIVE MEDICINE PI BIRMINGHAM PA 1209 MONTGOMERY HIGHWAY, BIRMINGHAM, AL 35216-2809 SN 0015-0282 J9 FERTIL STERIL JI Fertil. Steril. PD MAR PY 1991 VL 55 IS 3 BP 650 EP 651 PG 2 WC Obstetrics & Gynecology; Reproductive Biology SC Obstetrics & Gynecology; Reproductive Biology GA FA737 UT WOS:A1991FA73700040 ER PT J AU ALEXANDER, NJ AF ALEXANDER, NJ TI CORTICOID THERAPY - RISK BENEFIT - REPLY SO FERTILITY AND STERILITY LA English DT Letter RP ALEXANDER, NJ (reprint author), NICHHD,CONTRACEPT DEV BRANCH,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC REPRODUCTIVE MEDICINE PI BIRMINGHAM PA 1209 MONTGOMERY HIGHWAY, BIRMINGHAM, AL 35216-2809 SN 0015-0282 J9 FERTIL STERIL JI Fertil. Steril. PD MAR PY 1991 VL 55 IS 3 BP 652 EP 653 PG 2 WC Obstetrics & Gynecology; Reproductive Biology SC Obstetrics & Gynecology; Reproductive Biology GA FA737 UT WOS:A1991FA73700043 ER PT J AU BURTON, RW LYONS, JS DEVENS, M LARSON, DB AF BURTON, RW LYONS, JS DEVENS, M LARSON, DB TI PSYCHIATRIC CONSULTATIONS FOR PSYCHOACTIVE SUBSTANCE DISORDERS IN THE GENERAL-HOSPITAL SO GENERAL HOSPITAL PSYCHIATRY LA English DT Article ID LIAISON PSYCHIATRY; ALCOHOL; PATTERNS AB A series of 511 consultations to a psychiatric consultation-liaison service was studied. Patients diagnosed with substance abuse disorders were found more likely to be younger, male, and nonwhite. Payer status was not significantly different. Psychiatric consultants' recommendations for substance abuse patients more often consisted of aftercare referral, with less utilization of nonmedical consultations, environmental manipulations, social support, and psychological testing. Further, nonsubstance abusers received more and longer follow-up visits. Overall length of stay was shorter for substance-abuse cases, and for nonabusing cases earlier consultation was associated with briefer hospital stay. The authors discuss factors that may explain these findings, and urge psychiatric consultants to rethink their approach to these patients. A thorough, thoughtful diagnostic evaluation and consideration of a variety of interventions is always indicated. C1 NORTHWESTERN UNIV,SCH MED,CHICAGO,IL 60611. NIMH,BETHESDA,MD 20892. NR 16 TC 29 Z9 29 U1 2 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0163-8343 J9 GEN HOSP PSYCHIAT JI Gen. Hosp. Psych. PD MAR PY 1991 VL 13 IS 2 BP 83 EP 87 DI 10.1016/0163-8343(91)90018-R PG 5 WC Psychiatry SC Psychiatry GA FB979 UT WOS:A1991FB97900002 PM 2037246 ER PT J AU KLAR, AJS BONADUCE, MJ CAFFERKEY, R AF KLAR, AJS BONADUCE, MJ CAFFERKEY, R TI THE MECHANISM OF FISSION YEAST MATING TYPE INTERCONVERSION - SEAL REPLICATE CLEAVE MODEL OF REPLICATION ACROSS THE DOUBLE-STRANDED BREAK SITE AT MAT1 SO GENETICS LA English DT Article ID SCHIZOSACCHAROMYCES-POMBE; SWITCHING GENES; CASSETTES; FREQUENCY; TRANSPOSITION; RECOMBINATION; INITIATION; ASYMMETRY; REGION; CELLS AB The interconversion of cell type in the fission yeast, Schizosaccharomyces pombe, is initiated by a double-stranded break (DSB) found at the mating type locus (mat1). A heritable site- and strand-specific DNA "imprinting" event at mat1 was recently hypothesized to be required to make the mat1 locus cleavable, and the DSB was suggested to be produced one generation before the actual switching event. It is known that only one cell among four granddaughters of a cell ever switches, and the sister of the recently switched cell switches efficiently in consecutive cell divisions. The feature of consecutive switching creates a major difficulty of having to replicate chromosomes possessing the DSB. The mat1 cis-acting leaky mutation, called smt-s, reduces the level of the DSB required for switching and is shown here to be a 27-bp deletion located 50 bp away from the cut site. Determination of the pattern and frequency of switching of the mutant allele by cell lineage studies has allowed us to conclude the following: (1) the chromosome with the DSB is sealed and replicated, then one of the specific chromatids is cleaved again to generate switching-competent cells in consecutive cell divisions and (2) the smt-s mutation affects DNA cleavage and not the hypothesized DNA imprinting step. C1 COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724. RP KLAR, AJS (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74101]; NIGMS NIH HHS [GM25678] NR 31 TC 26 Z9 26 U1 0 U2 0 PU GENETICS PI BALTIMORE PA 428 EAST PRESTON ST, BALTIMORE, MD 21202 SN 0016-6731 J9 GENETICS JI Genetics PD MAR PY 1991 VL 127 IS 3 BP 489 EP 496 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA EZ708 UT WOS:A1991EZ70800004 PM 2016051 ER PT J AU CAWTHON, RM ANDERSEN, LB BUCHBERG, AM XU, GF OCONNELL, P VISKOCHIL, D WEISS, RB WALLACE, MR MARCHUK, DA CULVER, M STEVENS, J JENKINS, NA COPELAND, NG COLLINS, FS WHITE, R AF CAWTHON, RM ANDERSEN, LB BUCHBERG, AM XU, GF OCONNELL, P VISKOCHIL, D WEISS, RB WALLACE, MR MARCHUK, DA CULVER, M STEVENS, J JENKINS, NA COPELAND, NG COLLINS, FS WHITE, R TI CDNA SEQUENCE AND GENOMIC STRUCTURE OF EV12B, A GENE LYING WITHIN AN INTRON OF THE NEUROFIBROMATOSIS TYPE-1 GENE SO GENOMICS LA English DT Article ID GRADIENT GEL-ELECTROPHORESIS; POLYMERASE CHAIN-REACTION; T-CELL LYMPHOMAS; C-MYC; VONRECKLINGHAUSEN NEUROFIBROMATOSIS; CHROMOSOME-TRANSLOCATION; SIGNAL SEQUENCES; BURKITT-LYMPHOMA; POINT MUTATION; DNA-MOLECULES C1 UNIV MICHIGAN,DEPT INTERNAL MED,ANN ARBOR,MI 48109. UNIV MICHIGAN,DEPT HUMAN GENET,ANN ARBOR,MI 48109. UNIV MICHIGAN,HOWARD HUGHES MED INST,ANN ARBOR,MI 48109. NCI,FREDERICK CANC RES FACIL,CTR RES & DEV,ABL BASIC RES PROGRAM,FREDERICK,MD 21701. RP CAWTHON, RM (reprint author), UNIV UTAH,HOWARD HUGHES MED INST,SALT LAKE CITY,UT 84132, USA. OI Buchberg, Arthur/0000-0002-0543-5631 FU NCI NIH HHS [N01-CO-74101]; NINDS NIH HHS [NS23410] NR 81 TC 78 Z9 79 U1 0 U2 6 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD MAR PY 1991 VL 9 IS 3 BP 446 EP 460 DI 10.1016/0888-7543(91)90410-G PG 15 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA EY816 UT WOS:A1991EY81600005 PM 1903357 ER PT J AU BALLANTYNE, CM KOZAK, CA OBRIEN, WE BEAUDET, AL AF BALLANTYNE, CM KOZAK, CA OBRIEN, WE BEAUDET, AL TI ASSIGNMENT OF THE GENE FOR INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) TO PROXIMAL MOUSE CHROMOSOME-9 SO GENOMICS LA English DT Note ID RECEPTOR; ADHERENCE; ONCOGENE C1 BAYLOR UNIV,HOWARD HUGHES MED INST,HOUSTON,TX 77030. BAYLOR UNIV,INST MOLEC GENET,HOUSTON,TX 77030. NIAID,BETHESDA,MD 20814. RP BALLANTYNE, CM (reprint author), BAYLOR UNIV,DEPT MED,6565 FANNIN ST,MS A601,HOUSTON,TX 77030, USA. RI Ballantyne, Christie/A-6599-2008 NR 21 TC 22 Z9 22 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD MAR PY 1991 VL 9 IS 3 BP 547 EP 550 DI 10.1016/0888-7543(91)90423-C PG 4 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA EY816 UT WOS:A1991EY81600018 PM 1674500 ER PT J AU OLEARY, KM TURNER, ER GARDNER, DL COWDRY, RW AF OLEARY, KM TURNER, ER GARDNER, DL COWDRY, RW TI HOMOGENEOUS GROUP-THERAPY OF BORDERLINE PERSONALITY-DISORDER SO GROUP LA English DT Article ID GROUP-PSYCHOTHERAPY; NARCISSISTIC PATIENTS; INDIVIDUAL TREATMENT RP OLEARY, KM (reprint author), NIMH,CTR NEUROSCI,ADM OFF,WASHINGTON,DC 20032, USA. NR 23 TC 1 Z9 1 U1 0 U2 0 PU HUMAN SCI PRESS INC PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013-1578 SN 0362-4021 J9 GROUP JI Group PD SPR PY 1991 VL 15 IS 1 BP 56 EP 64 DI 10.1007/BF01419846 PG 9 WC Psychology, Clinical SC Psychology GA FJ229 UT WOS:A1991FJ22900006 ER PT J AU RICE, M VALDIVIA, L AF RICE, M VALDIVIA, L TI A SIMPLE GUIDE FOR DESIGN, USE, AND EVALUATION OF EDUCATIONAL-MATERIALS SO HEALTH EDUCATION QUARTERLY LA English DT Article C1 NIH,ALCOHOL DRUG ABUSE & MENTAL HLTH ADM,OFF SUBST ABUSE PREVENT,BETHESDA,MD 20892. PAN AMER HLTH ORG,WASHINGTON,DC. NR 2 TC 14 Z9 17 U1 0 U2 0 PU SAGE SCIENCE PRESS PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 SN 0195-8402 J9 HEALTH EDUC QUART JI Health Educ. Q. PD SPR PY 1991 VL 18 IS 1 BP 79 EP 85 DI 10.1177/109019819101800108 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FA891 UT WOS:A1991FA89100008 PM 2037504 ER PT J AU DIBISCEGLIE, AM HOOFNAGLE, JH AF DIBISCEGLIE, AM HOOFNAGLE, JH TI THERAPY OF CHRONIC HEPATITIS-C WITH ALPHA-INTERFERON - THE ANSWER - OR MORE QUESTIONS SO HEPATOLOGY LA English DT Editorial Material ID NON-B-HEPATITIS; NON-A-HEPATITIS; CONTROLLED TRIAL; ALFA THERAPY; VIRUS C1 NIDDKD,DIV DIGEST DIS & NUTR,BETHESDA,MD 20892. RP DIBISCEGLIE, AM (reprint author), NIH,DIGEST DIS BRANCH,LIVER DIS SECT,BLDG 10,ROOM 4D52,BETHESDA,MD 20892, USA. NR 23 TC 61 Z9 61 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD MAR PY 1991 VL 13 IS 3 BP 601 EP 603 DI 10.1016/0270-9139(91)90318-P PG 3 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA FC524 UT WOS:A1991FC52400031 PM 1900256 ER PT J AU CORNETTA, K MORGAN, RA ANDERSON, WF AF CORNETTA, K MORGAN, RA ANDERSON, WF TI SAFETY ISSUES RELATED TO RETROVIRAL-MEDIATED GENE-TRANSFER IN HUMANS SO HUMAN GENE THERAPY LA English DT Review ID MURINE LEUKEMIA VIRUSES; TYROSINE PROTEIN-KINASE; FOCUS-FORMING VIRUSES; HUMAN-BREAST CANCER; VL30 DNA-SEQUENCES; A-PARTICLE GENES; C-MYC; COLORECTAL CARCINOMAS; NUCLEOTIDE-SEQUENCE; NONDEFECTIVE FRIEND AB The first three approved human clinical trials utilizing retroviral-mediated gene transfer are now underway. While this technology holds great promise for the study and treatment of human disease, it also poses a number of safety concerns. In evaluating clinical protocols, potential complications and the likelihood of their occurrence are estimated by review committees so that a risk/benefit assessment can be made. Current knowledge, reviewed in this article, suggests that no acute complications secondary to retroviral-mediated gene transfer are likely, but the possibility of long-term or unforeseen sequelae in patients suggests the need for post-treatment monitoring. C1 NHLBI,MOLEC HEMATOL BRANCH,BETHESDA,MD 20892. RI Ain, Kenneth/A-5179-2012 OI Ain, Kenneth/0000-0002-2668-934X NR 76 TC 204 Z9 209 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1043-0342 J9 HUM GENE THER JI Hum. Gene Ther. PD SPR PY 1991 VL 2 IS 1 BP 5 EP 14 DI 10.1089/hum.1991.2.1-5 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA FJ889 UT WOS:A1991FJ88900003 PM 1863639 ER PT J AU SALMINEN, A ELSON, HF MICKLEY, LA FOJO, AT GOTTESMAN, MM AF SALMINEN, A ELSON, HF MICKLEY, LA FOJO, AT GOTTESMAN, MM TI IMPLANTATION OF RECOMBINANT RAT MYOCYTES INTO ADULT SKELETAL-MUSCLE - A POTENTIAL GENE-THERAPY SO HUMAN GENE THERAPY LA English DT Article ID MULTIDRUG-RESISTANCE GENE; HUMAN MDR1 GENE; INSITU HYBRIDIZATION; P-GLYCOPROTEIN; EXPRESSION; CELLS; DYSTROPHIN; RETROVIRUSES; INJECTION; MICE AB The ability of skeletal muscle to regenerate provides an excellent therapeutic entry, via genetic engineering, for correcting diseases of skeletal muscle and other tissues. We have used a retrovirus to transfer the cDNA for the human multidrug transporter, encoded by the MDR1 gene, into the genomes of the rat muscle cell line L6 and into primary rat myocytes. The MDR1 gene confers drug resistance to cells, and thus serves as a selectable marker in vitro. In cultured cells, the retroviral promoter-driven human MDR1 cDNA was shown to be stable in the presence or absence of drug selection or muscle cell fusion. MDR1 mRNA was synthesized, as shown by RNA blot analysis and in situ hybridization. The protein product was localized to the plasma membrane of transduced myocytes and myotubes by immunofluorescence. As a model for skeletal muscle gene therapy, transduced L6 myocytes were implanted into the tibialis anterior muscle of Wistar rats. The retroviral sequences of the human MDR1 gene and its mRNA were present in the muscles of Wistar rats 5 days, but not 12 days, after implantation, possibly because of immunorejection. On the other hand, the human MDR1 cDNA was stable in the tibialis anterior muscle of nude mice, which are incapable of immunorejection, at least 4 weeks after implantation of myocytes. Immunosuppression of Wistar rats with cyclosporine A delayed immunorejection of recombinant myocytes, and MDR1 cDNA and mRNA was detected 3-4 weeks after implantation. In situ hybridization revealed that injected recombinant myocytes remain in discrete foci in adult rodent skeletal muscle and express MDR1 mRNA for at least 30 days in nude mice and cyclosporine-treated rats. C1 NCI,DIV CANC BIOL DIAG CTR,CELL BIOL LAB,BLDG 37,ROOM 2E18,BETHESDA,MD 20892. NCI,DIV CANC TREATMENT,MED BRANCH,BETHESDA,MD 20892. NR 37 TC 23 Z9 23 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1043-0342 J9 HUM GENE THER JI Hum. Gene Ther. PD SPR PY 1991 VL 2 IS 1 BP 15 EP 26 DI 10.1089/hum.1991.2.1-15 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA FJ889 UT WOS:A1991FJ88900004 PM 1677815 ER PT J AU ROSENBRONSON, S JARAQUEMADA, D AF ROSENBRONSON, S JARAQUEMADA, D TI ON THE RELATIVE IMMUNOGENICITY OF DR ALLOANTIGENS - T-CELL RECOGNITION OF HLA-DR2A AND HLA-DR2B SO HUMAN IMMUNOLOGY LA English DT Article ID MOUSE L-CELLS; GENE-TRANSFER; ANTIGENS; MOLECULES; SUBTYPES; PRODUCTS; PEPTIDE; FAMILY; CLONES; BETA AB The HLA-DR2 haplotype encodes two highly polymorphic DR molecules, DR2a and DR2b. Because little is known regarding the relative immunogenicity of different HLA-DR molecules, we have studied the T-cell recognition of DR2a and DR2b molecules from the DRw15, Dw2 haplotype. A series of DR2-specific alloreactive T-cell clones were analyzed with murine L-cell transfectants expressing either the DR2a or the DR2b molecules as stimulator cells in proliferation assays. Somewhat surprisingly, both DR2a and DR2b were capable of stimulating DR2-specific T-cell clones with equal magnitude and similar frequency. In addition, DR2a and DR2b are functionally distinct, that is, no clone was identified which was stimulated by both DR2a and DR2b molecules. C1 NIAID,IMMUNOGENET LAB,BETHESDA,MD 20892. GEORGETOWN UNIV,SCH MED,DEPT PEDIAT,WASHINGTON,DC 20057. NR 27 TC 12 Z9 12 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0198-8859 J9 HUM IMMUNOL JI Hum. Immunol. PD MAR PY 1991 VL 30 IS 3 BP 215 EP 221 DI 10.1016/0198-8859(91)90037-A PG 7 WC Immunology SC Immunology GA EZ652 UT WOS:A1991EZ65200009 PM 2055786 ER PT J AU BLACK, HR CURB, JD PRESSEL, S PROBSTFIELD, J STAMLER, J AF BLACK, HR CURB, JD PRESSEL, S PROBSTFIELD, J STAMLER, J TI SYSTOLIC HYPERTENSION IN THE ELDERLY PROGRAM (SHEP) - INTRODUCTION SO HYPERTENSION LA English DT Editorial Material C1 NHLBI,DECA,CLIN TRIALS BRANCH,FED BLDG,ROOM 5C10,7550 WISCONSIN AVE,BETHESDA,MD 20892. NR 0 TC 14 Z9 14 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0194-911X J9 HYPERTENSION JI Hypertension PD MAR PY 1991 VL 17 IS 3 SU S BP 1 EP 1 PG 1 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA FB314 UT WOS:A1991FB31400001 ER PT J AU BORHANI, NO APPLEGATE, WB CUTLER, JA DAVIS, BR FURBERG, CD LAKATOS, E PAGE, L PERRY, HM SMITH, WM PROBSTFIELD, JL AF BORHANI, NO APPLEGATE, WB CUTLER, JA DAVIS, BR FURBERG, CD LAKATOS, E PAGE, L PERRY, HM SMITH, WM PROBSTFIELD, JL TI RATIONALE AND DESIGN SO HYPERTENSION LA English DT Article ID ISOLATED SYSTOLIC HYPERTENSION; FACTOR INTERVENTION TRIAL; BLOOD-PRESSURE; MORTALITY; RISK; DISEASE; POPULATION; MORBIDITY; MRFIT; CARE C1 NHLBI,DECA,CLIN TRIALS BRANCH,FED BLDG,ROOM 5C10,7550 WISCONSIN AVE,BETHESDA,MD 20892. NR 51 TC 31 Z9 31 U1 0 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0194-911X J9 HYPERTENSION JI Hypertension PD MAR PY 1991 VL 17 IS 3 SU S BP 2 EP 15 PG 14 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA FB314 UT WOS:A1991FB31400002 ER PT J AU PETROVITCH, H BYINGTON, R BAILEY, G BORHANI, P CARMODY, S GOODWIN, L HARRINGTON, J JOHNSON, HA JOHNSON, P JONES, M LEVIN, J SUGARS, C PROBSTFIELD, JL AF PETROVITCH, H BYINGTON, R BAILEY, G BORHANI, P CARMODY, S GOODWIN, L HARRINGTON, J JOHNSON, HA JOHNSON, P JONES, M LEVIN, J SUGARS, C PROBSTFIELD, JL TI SCREENING AND RECRUITMENT SO HYPERTENSION LA English DT Article ID SYSTOLIC HYPERTENSION C1 NHLBI,DECA,CLIN TRIALS BRANCH,FED BLDG,ROOM 5010,7550 WISCONSIN AVE,BETHESDA,MD 20892. NR 6 TC 38 Z9 38 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0194-911X J9 HYPERTENSION JI Hypertension PD MAR PY 1991 VL 17 IS 3 SU S BP 16 EP 23 PG 8 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA FB314 UT WOS:A1991FB31400003 ER PT J AU MOCKRIN, SC DZAU, VJ GROSS, KW HORAN, MJ AF MOCKRIN, SC DZAU, VJ GROSS, KW HORAN, MJ TI TRANSGENIC ANIMALS - NEW APPROACHES TO HYPERTENSION RESEARCH SO HYPERTENSION LA English DT Article DE TRANSGENIC ANIMALS; BLOOD PRESSURE; RESEARCH; MOLECULAR BIOLOGY ID RENIN GENES; MICE; EXPRESSION; DISEASE; MOUSE; TUMORS; ALPHA-1-ANTITRYPSIN; ABLATION; SYSTEM; GROWTH RP MOCKRIN, SC (reprint author), NHLBI,DIV HEART & VASC DIS,HYPERTENS & KIDNEY DIS,BETHESDA,MD 20892, USA. NR 48 TC 21 Z9 21 U1 0 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0194-911X J9 HYPERTENSION JI Hypertension PD MAR PY 1991 VL 17 IS 3 BP 394 EP 399 PG 6 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA FA882 UT WOS:A1991FA88200019 PM 1999368 ER PT J AU UNSER, M ALDROUBI, A EDEN, M AF UNSER, M ALDROUBI, A EDEN, M TI RECURSIVE REGULARIZATION FILTERS - DESIGN, PROPERTIES, AND APPLICATIONS SO IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE LA English DT Note DE APPROXIMATION METHODS; EDGE DETECTION; GAUSSIAN FILTERING; RECURSIVE FILTERS; REGULARIZATION; SMOOTHING ID EDGE-DETECTION; EXTRACTION; VISION AB Least squares approximation problems that are regularized with specified highpass stabilizing kernels are considered. For each problem, there is a family of discrete regularization filters (R-filters) allowing an efficient determination of the solutions. These operators are stable symmetric lowpass filters with an adjustable scale factor. Two decomposition theorems for the z-transform of such systems are presented. One facilitates the determination of their impulse response, while the other allows an efficient implementation through successive causal and anticausal recursive filtering. A case of special interest is the design of R-filters for the first and second order difference operators. These results are extended for two-dimensional signals and, for illustration purposes, are applied to the problem of edge detection. This leads to a very efficient implementation (8 multiplies + 10 adds per pixel) of the optimal Canny edge detector based on the use of a separable second order R-filter. C1 HOP HENRI MONDOR,INSERM,UNITE 2,F-94010 CRETEIL,FRANCE. RP UNSER, M (reprint author), NIH,BIOMED & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892, USA. RI Unser, Michael/A-1550-2008; Aldroubi, Akram/J-7186-2012 NR 23 TC 21 Z9 23 U1 1 U2 1 PU IEEE COMPUTER SOC PI LOS ALAMITOS PA 10662 LOS VAQUEROS CIRCLE, PO BOX 3014, LOS ALAMITOS, CA 90720-1264 SN 0162-8828 J9 IEEE T PATTERN ANAL JI IEEE Trans. Pattern Anal. Mach. Intell. PD MAR PY 1991 VL 13 IS 3 BP 272 EP 277 DI 10.1109/34.75514 PG 6 WC Computer Science, Artificial Intelligence; Engineering, Electrical & Electronic SC Computer Science; Engineering GA FG360 UT WOS:A1991FG36000006 ER PT J AU UNSER, M ALDROUBI, A EDEN, M AF UNSER, M ALDROUBI, A EDEN, M TI FAST B-SPLINE TRANSFORMS FOR CONTINUOUS IMAGE REPRESENTATION AND INTERPOLATION SO IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE LA English DT Note DE B-SPLINES; CONTINUOUS REPRESENTATION; IMAGE RECONSTRUCTION; INTERPOLATION; POLYNOMIAL SPLINES; RECURSIVE FILTER; TRANSFORM AB This correspondence describes efficient algorithms for the continuous representation of a discrete signal in terms of B-splines (direct B-spline transform), and for interpolative signal reconstruction (indirect B-spline transform) with an expansion factor m. Expressions for the z-transforms of the sampled B-spline functions are determined and a convolution property of these kernels is established. It is shown that both the direct and indirect spline transforms involve linear operators that are translation invariant and are implemented efficiently by linear filtering. Fast computational algorithms based on the recursive implementation of these filters are proposed. A B-spline interpolator can also be characterized in terms of its transfer function and its global impulse response (cardinal spline of order n). The case of the cubic spline is treated in greater detail. The present approach is compared with previous methods that are reexamined from a critical point of view. We conclude that, contrary to the claims of several authors, B-spline interpolation correctly applied does not result in a loss of image resolution and that this type of interpolation can be performed in a very efficient manner. C1 HOP HENRI MONDOR,INSERM,UNITE 2,F-94010 CRETEIL,FRANCE. RP UNSER, M (reprint author), NIH,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892, USA. RI Unser, Michael/A-1550-2008; Aldroubi, Akram/J-7186-2012 NR 20 TC 288 Z9 313 U1 1 U2 18 PU IEEE COMPUTER SOC PI LOS ALAMITOS PA 10662 LOS VAQUEROS CIRCLE, PO BOX 3014, LOS ALAMITOS, CA 90720-1264 SN 0162-8828 J9 IEEE T PATTERN ANAL JI IEEE Trans. Pattern Anal. Mach. Intell. PD MAR PY 1991 VL 13 IS 3 BP 277 EP 285 DI 10.1109/34.75515 PG 9 WC Computer Science, Artificial Intelligence; Engineering, Electrical & Electronic SC Computer Science; Engineering GA FG360 UT WOS:A1991FG36000007 ER PT J AU FINKELMAN, FD PEARCE, EJ URBAN, JF SHER, A AF FINKELMAN, FD PEARCE, EJ URBAN, JF SHER, A TI REGULATION AND BIOLOGICAL FUNCTION OF HELMINTH-INDUCED CYTOKINE RESPONSES SO IMMUNOPARASITOLOGY TODAY-A COMBINED ISSUE OF IMMUNOLOGY TODAY AND PARASITOLOGY TODAY LA English DT Article ID STIMULATORY FACTOR-I; T-CELL; INTERFERON-GAMMA; IFN-GAMMA; NIPPOSTRONGYLUS-BRASILIENSIS; LYMPHOKINE SECRETION; TRICHINELLA-SPIRALIS; HUMAN-LYMPHOCYTES; IMMUNE-RESPONSES; IGE RESPONSE C1 USDA ARS,BELTSVILLE AGR RES CTR,INST LIVESTOCK & POULTRY SCI,HELMINTH DIS LAB,BELTSVILLE,MD 20705. NIAID,PARASIT DIS LAB,IMMUNOL & CELL BIOL SECT,BETHESDA,MD 20892. RP FINKELMAN, FD (reprint author), UNIFORMED SERV UNIV HLTH SCI,DEPT MED,DIV RHEUMATOL & IMMUNOL,BETHESDA,MD 20889, USA. NR 50 TC 57 Z9 57 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0167-4919 J9 IMMUNOPARASITOL TOD PD MAR PY 1991 IS 3 BP A62 EP A66 PG 5 WC Immunology; Parasitology SC Immunology; Parasitology GA FG877 UT WOS:A1991FG87700018 ER PT J AU KING, CL NUTMAN, TB AF KING, CL NUTMAN, TB TI REGULATION OF THE IMMUNE-RESPONSE IN LYMPHATIC FILARIASIS AND ONCHOCERCIASIS SO IMMUNOPARASITOLOGY TODAY-A COMBINED ISSUE OF IMMUNOLOGY TODAY AND PARASITOLOGY TODAY LA English DT Article ID BRUGIA-MALAYI; IMMUNOLOGICAL ASPECTS; ENDEMIC FILARIASIS; ANTIFILARIAL IGG; PARASITE ANTIGEN; PACIFIC ISLAND; ANTIBODIES; INFECTION; DIETHYLCARBAMAZINE; IMMUNOGLOBULIN RP KING, CL (reprint author), NIAID,PARASIT DIS LAB,BETHESDA,MD 20892, USA. NR 49 TC 19 Z9 19 U1 1 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0167-4919 J9 IMMUNOPARASITOL TOD PD MAR PY 1991 IS 3 BP A54 EP A58 PG 5 WC Immunology; Parasitology SC Immunology; Parasitology GA FG877 UT WOS:A1991FG87700016 ER PT J AU ARORA, PK HANNA, EE SKOLNICK, P AF ARORA, PK HANNA, EE SKOLNICK, P TI SUPPRESSION OF CYTOTOXIC LYMPHOCYTE-T (CTL) ACTIVITY BY FG-7142, A BENZODIAZEPINE RECEPTOR INVERSE AGONIST SO IMMUNOPHARMACOLOGY LA English DT Article DE CYTOTOXIC LYMPHOCYTE-T; BENZODIAZEPINE RECEPTOR; CENTRAL NERVOUS SYSTEM; FG-7142; RO-15-1788 ID IMMUNE-RESPONSE; BINDING-SITES; GENETIC-CONTROL; CELLS; ANXIETY; BRAIN; RAT; INNERVATION; ENHANCE; LIGAND AB A dose-dependent (12.5-100 mg/kg) suppression of cytotoxic T lymphocyte (CTL) activity was observed in mice after administration of the benzodiazepine receptor 'inverse agonist' FG 7142 (N-methyl-beta-carboline-3-carboxamide). This compound produces a syndrome resembling stress or anxiety in both animals and man. Addition of FG 7142 (1-1000 nM) to either a 4-hour Chromium-51-release assay or 5-day in vitro CTL generation system did not affect CTL activity. Pretreatment with the benzodiazepine receptor antagonist Ro 15-1788 (10 mg/kg) attenuated FG 7142-induced suppression of CTL activity, but had no effect when administered alone. Time-course studies indicated that FG 7142-induced suppression of CTL activity was long-lasting. The suppression of CTL activity by FG 7142 provides further evidence that the central nervous system pathways subserved by the benzodiazepine/GABA receptor chloride channel complex may play an important role in the modulation of immune function. C1 NICHHD,DEV & MOLEC IMMUN LAB,BETHESDA,MD. RP ARORA, PK (reprint author), NIDDK,NEUROSCI LAB,BLDG 8,RM 111,BETHESDA,MD 20892, USA. NR 35 TC 12 Z9 12 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0162-3109 J9 IMMUNOPHARMACOLOGY JI Immunopharmacology PD MAR-APR PY 1991 VL 21 IS 2 BP 91 EP 97 DI 10.1016/0162-3109(91)90012-N PG 7 WC Immunology; Pharmacology & Pharmacy SC Immunology; Pharmacology & Pharmacy GA FL958 UT WOS:A1991FL95800003 PM 1652574 ER PT J AU GANESHKUMAR, N HANNAM, PM KOLENBRANDER, PE MCBRIDE, BC AF GANESHKUMAR, N HANNAM, PM KOLENBRANDER, PE MCBRIDE, BC TI NUCLEOTIDE-SEQUENCE OF A GENE CODING FOR A SALIVA-BINDING PROTEIN (SSAB) FROM STREPTOCOCCUS-SANGUIS 12 AND POSSIBLE ROLE OF THE PROTEIN IN COAGGREGATION WITH ACTINOMYCES SO INFECTION AND IMMUNITY LA English DT Article ID ESCHERICHIA-COLI; COATED HYDROXYAPATITE; BACTERIAL ADHERENCE; BACILLUS-SUBTILIS; TYPE-1 FIMBRIAE; CLONING; COLONIZATION; INITIATION; ADHESION; ECOLOGY AB The nucleotide sequence of a 2.9-kb streptococcal DNA fragment which codes for two proteins with M(r)s of 36,000 (Streptococcus sanguis adhesin B [SsaB]) and 20,000 has been determined. The ssaB gene is 927 bp and codes for a 34,684-Da protein. The open reading frame coding for the 20-kDa protein is 489 bp and codes for a protein of 17,885 Da. The SsaB protein has a putative hydrophobic 19-amino-acid signal sequence resulting in a 32,620-M(r) secreted protein, whereas the 20-kDa protein has no signal sequence. Both proteins are hydrophilic, and neither appears to have a hydrophobic membrane anchor sequence in the carboxy-terminal region. A DNA sequence homology of 73% exists between the cloned fragment containing the ssaB gene from S. sanguis 12 and the cloned fragment containing the type 1 fimbrial gene of S. sanguis FW213 (J. C. Fenno, D. J. LeBlanc, and P. Fives-Taylor, Infect. Immun. 57:3527-3533, 1989). Amino acid comparisons of the SsaB and type 1 fimbrial proteins show 87% homology, indicating a close similarity of the two proteins. Antiserum raised against the cloned SsaB protein cross-reacts with a 38-kDa protein identified from Streptococcus gordonii (S. sanguis) PK488 which was proposed to mediate coaggregation with Actinomyces naeslundii PK606 (P. E. Kolenbrander and R. N. Andersen, Infect. Immun. 58:3064-3072, 1990). The SsaB adhesin may play a role in oral colonization by binding either to a receptor on saliva or to a receptor on actinomyces. C1 UNIV BRITISH COLUMBIA,DEPT MICROBIOL,VANCOUVER V6T 1W5,BC,CANADA. UNIV BRITISH COLUMBIA,DEPT ORAL BIOL,VANCOUVER V6T 1W5,BC,CANADA. RP GANESHKUMAR, N (reprint author), NIDR,MICROBIAL ECOL LAB,BETHESDA,MD 20892, USA. NR 38 TC 81 Z9 86 U1 1 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD MAR PY 1991 VL 59 IS 3 BP 1093 EP 1099 PG 7 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA EZ572 UT WOS:A1991EZ57200051 PM 1671775 ER PT J AU BORNSTEIN, MH TAMISLEMONDA, CS PECHEUX, MG RAHN, CW AF BORNSTEIN, MH TAMISLEMONDA, CS PECHEUX, MG RAHN, CW TI MOTHER AND INFANT ACTIVITY AND INTERACTION IN FRANCE AND IN THE UNITED-STATES - A COMPARATIVE-STUDY SO INTERNATIONAL JOURNAL OF BEHAVIORAL DEVELOPMENT LA English DT Article ID CHILD; LANGUAGE; COMMUNICATION; HABITUATION; COMPETENCE; PSYCHOLOGY; STABILITY; CULTURE; PLAY C1 NYU,NEW YORK,NY 10003. UNIV PARIS 05,F-75270 PARIS 06,FRANCE. RP BORNSTEIN, MH (reprint author), NICHHD,CHILD & FAMILY RES SECT,BLDG 31,ROOM B2B15,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 121 TC 22 Z9 22 U1 2 U2 3 PU PSYCHOLOGY PRESS PI HOVE PA 27 CHURCH RD, HOVE, EAST SUSSEX, ENGLAND BN3 2FA SN 0165-0254 J9 INT J BEHAV DEV JI Int. J. Behav. Dev. PD MAR PY 1991 VL 14 IS 1 BP 21 EP 43 PG 23 WC Psychology, Developmental SC Psychology GA FC105 UT WOS:A1991FC10500002 ER PT J AU STERNBERG, KJ LAMB, ME HWANG, CP BROBERG, A KETTERLINUS, RD BOOKSTEIN, FL AF STERNBERG, KJ LAMB, ME HWANG, CP BROBERG, A KETTERLINUS, RD BOOKSTEIN, FL TI DOES OUT-OF-HOME CARE AFFECT COMPLIANCE IN PRESCHOOLERS SO INTERNATIONAL JOURNAL OF BEHAVIORAL DEVELOPMENT LA English DT Article ID INFANT DAY-CARE; YOUNG-CHILDREN; AGGRESSION; EXPERIENCE; ATTACHMENT; BEHAVIOR; QUALITY C1 GOTHENBURG UNIV,S-41124 GOTHENBURG,SWEDEN. UNIV MICHIGAN,ANN ARBOR,MI 48109. RP STERNBERG, KJ (reprint author), NICHHD,9190 ROCKVILLE PIKE,BSA BLDG,ROOM 331,BETHESDA,MD 20814, USA. NR 43 TC 17 Z9 17 U1 1 U2 2 PU PSYCHOLOGY PRESS PI HOVE PA 27 CHURCH RD, HOVE, EAST SUSSEX, ENGLAND BN3 2FA SN 0165-0254 J9 INT J BEHAV DEV JI Int. J. Behav. Dev. PD MAR PY 1991 VL 14 IS 1 BP 45 EP 65 PG 21 WC Psychology, Developmental SC Psychology GA FC105 UT WOS:A1991FC10500003 ER PT J AU HSING, AW GUO, WD CHEN, JS LI, JY STONE, BJ BLOT, WJ FRAUMENI, JF AF HSING, AW GUO, WD CHEN, JS LI, JY STONE, BJ BLOT, WJ FRAUMENI, JF TI CORRELATES OF LIVER-CANCER MORTALITY IN CHINA SO INTERNATIONAL JOURNAL OF EPIDEMIOLOGY LA English DT Article ID HEPATITIS-B VIRUS; PRIMARY HEPATOCELLULAR-CARCINOMA; TAIWAN; RISK; CONSUMPTION; AFLATOXIN; MORBIDITY; ETIOLOGY; ALCOHOL AB To provide clues to the causes of liver cancer in China, we studied the correlation of certain dietary and biochemical markers with liver cancer mortality across 65 Chinese countries. Mortality rates were significantly linked to the county-wide prevalence of hepatitis-B surface antigen positivity. Rates were also higher in countries with high plasma levels of total cholesterol and high consumption of liquor, rapeseed oil, and mouldy corn, while inverse associations were observed for wheat consumption. All of the observed associations, except those with cholesterol and rapeseed oil, were more pronounced in men than in women. No significant correlations with liver cancer mortality were found for consumption of several other foods; plasma levels of retinol, beta-carotene, alpha-tocopherol, selenium, zinc and ferritin; or urine levels of aflatoxin B1. Although causal inferences cannot be derived, this ecological study suggests that chronic infection with hepatitis-B virus contributes to the substantial variation in liver cancer mortality in China, and provides leads for further studies into the role of dietary and nutritional determinants. C1 CHINESE ACAD MED SCI,INST CANC,BEIJING,PEOPLES R CHINA. CHINESE ACAD PREVENT MED,INST NUTR & FOOD HYG,BEIJING,PEOPLES R CHINA. RP HSING, AW (reprint author), NCI,EXECUT PLAZA N,ROOM 415,BETHESDA,MD 20892, USA. NR 29 TC 18 Z9 18 U1 0 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0300-5771 J9 INT J EPIDEMIOL JI Int. J. Epidemiol. PD MAR PY 1991 VL 20 IS 1 BP 54 EP 59 DI 10.1093/ije/20.1.54 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA FK354 UT WOS:A1991FK35400009 PM 2066244 ER PT J AU YAMINI, S STAPLES, RC HANSEN, CT SZEPESI, B AF YAMINI, S STAPLES, RC HANSEN, CT SZEPESI, B TI EFFECT OF DIETARY CARBOHYDRATE ON LIVER AND KIDNEY ENZYME-ACTIVITIES AND PLASMA AMINO-ACIDS IN THE LA/N-CP RAT SO INTERNATIONAL JOURNAL OF OBESITY LA English DT Article DE DIETARY CARBOHYDRATE; LIVER; KIDNEY; PLASMA AMINO ACIDS; RAT ID OBESE ZUCKER RATS; SMALL-INTESTINE; FATTY RAT; METABOLISM; SUCROSE; CORPULENT; INSULIN; PARAMETERS; EFFICIENCY; RESPONSES AB Twenty obese and 20 lean LA/N-cp male rats and 20 male Sprague-Dawley rats were fed a diet containing either 54 percent sucrose or starch for six weeks. After a 14-16 hour fast, rats were killed. Liver and kidney enzyme activities were determined in the LA/N-cp rats while plasma urea and selected amino acids were determined in all rats. Liver glucose-6-phosphatase (G6PASE), fructose-1,6-bisphosphatase (FBPASE), phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), malic enzyme (ME), glucokinase (GK), pyruvate kinase (PK), phosphofructokinase (PFK), glutamic-oxaloacetic-transaminase (GOT), glutamic-pyruvic transaminase (GPT), arginase (ARGASE), arginine-synthase (ARG-SYN) and ornithine transcarbamylase (OTC) levels were significantly affected by phenotype (obese > lean). All the above changes in enzyme levels were exaggerated by sucrose-feeding with the exception of PK, PFK, GOT, GPT, ARGASE and ARG-SYN. Kidney cortex G6PASE, PEPCK and ARGASE activities were higher in the obese rats as compared to the lean littermates. Sucrose feeding resulted in higher cortex G6PASE, FBPASE and PEPCK as compared to starch-fed rats. A phenotype effect was noted with plasma glutamate, urea, leucine, isoleucine and valine (obese > lean) and a diet effect was seen with aspartate, phenylalanine, leucine and valine (sucrose > starch) concentration. Sprague-Dawley rats had higher plasma urea and lower alanine than lean LA/N-cp males. Metabolic obesity in the LA/N-cp rat appears to involve an elevated capacity for pathways of glycolysis, gluconeogenesis, lipogenesis and amino acid catabolism in the liver. C1 UNIV MARYLAND,DEPT HUMAN NUTR & FOOD SYST,COLLEGE PK,MD 20742. NIH,DIV RES SERV,SMALL ANIM SECT,VET RESOURCES BRANCH,BETHESDA,MD 20892. RP YAMINI, S (reprint author), USDA ARS,BELTSVILLE AGR RES CTR,BELTSVILLE HUMAN NUTR RES CTR,CARBOHYDRATE NUTR LAB,BLDG 307,BELTSVILLE,MD 20705, USA. NR 35 TC 8 Z9 8 U1 0 U2 2 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0307-0565 J9 INT J OBESITY JI Int. J. Obes. PD MAR PY 1991 VL 15 IS 3 BP 189 EP 203 PG 15 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA FA872 UT WOS:A1991FA87200002 PM 2045212 ER PT J AU KINSELLA, TJ MISER, JS WALLER, B VENZON, D GLATSTEIN, E WEAVERMCCLURE, L HOROWITZ, ME AF KINSELLA, TJ MISER, JS WALLER, B VENZON, D GLATSTEIN, E WEAVERMCCLURE, L HOROWITZ, ME TI LONG-TERM FOLLOW-UP OF EWINGS-SARCOMA OF BONE TREATED WITH COMBINED MODALITY THERAPY SO INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS LA English DT Article; Proceedings Paper CT 31ST ANNUAL MEETING OF THE AMERICAN SOC FOR THERAPEUTIC RADIOLOGY AND ONCOLOGY CY OCT 01-06, 1989 CL SAN FRANCISCO, CA SP AMER SOC THERAPEUT RADIOL & ONCOL DE EWINGS SARCOMA; COMBINED MODALITY TREATMENT ID YOUNG-ADULTS; RADIATION-THERAPY; CHILDREN; CHEMOTHERAPY; EXPERIENCE; TUMORS AB Between 1968 and 1980, 107 consecutive patients with Ewing's sarcoma of bone were entered on three sequential combined modality treatment protocols (S2, S3, S4) at the National Cancer Institute (NCI). Protocol treatment involved 4 cycles of two drug [cyclophosphamide (CTX) and vincristine (VCR)] or three drug [CTX and VCR with either actinomycin-D (ACT-D) or doxorubicin (ADR)] regimens and local irradiation (50 Gy) to the involved bone. Eighty patients presented with localized disease and 27 patients had metastatic disease at presentation, including 11 patients with multiple metastatic sites. With a median potential follow-up of > 15 yrs (range 8-20 yrs), 28 pts (27%) remain alive. Disease-free (DFS) and overall survival (OS) decreased most rapidly during the initial 5 yrs of follow-up with 5-yr DFS of 29% and 5-yr OS of 39%. Only two patients with metastases at presentation are long term (> 5 yr) survivors. For localized disease patients, the 2, 5, 10, and 15 yr DFS and OS are 52%, 37%, 35%, and 33% DFS and 68%, 51%, 39%, and 34% OS, respectively. Eleven patients relapsed locally as the first site of failure. Using the Cox proportional hazards model, four significant variables for both DFS and OS were recognized, including metastatic disease at presentation, age > 25 yrs, high LDH in localized disease patients, and central primary tumor in localized disease patients in decreasing order of significance. We conclude that a majority of these patients with Ewing's sarcoma of bone relapsed within 5 yrs of presentation although late relapse (5-15 yrs) did occur. Local failure occurred in 20% of patients using these combined modality treatments but had no impact on overall survival. C1 NCI,CLIN ONCOL PROGRAM,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. NCI,CLIN ONCOL PROGRAM,PEDIAT BRANCH,BETHESDA,MD 20892. NCI,CLIN ONCOL PROGRAM,BIOSTAT & DATA MANAGEMENT SECT,BETHESDA,MD 20892. RP KINSELLA, TJ (reprint author), UNIV WISCONSIN,CTR CLIN CANC,DEPT HUMAN ONCOL,600 HIGHLAND AVE,K4-312,MADISON,WI 53792, USA. RI Venzon, David/B-3078-2008 NR 32 TC 82 Z9 82 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0360-3016 J9 INT J RADIAT ONCOL JI Int. J. Radiat. Oncol. Biol. Phys. PD MAR PY 1991 VL 20 IS 3 BP 389 EP 395 PG 7 WC Oncology; Radiology, Nuclear Medicine & Medical Imaging SC Oncology; Radiology, Nuclear Medicine & Medical Imaging GA EZ900 UT WOS:A1991EZ90000001 PM 1995522 ER PT J AU KANEMOTO, T MARTIN, GR HAMILTON, TC FRIDMAN, R AF KANEMOTO, T MARTIN, GR HAMILTON, TC FRIDMAN, R TI EFFECTS OF SYNTHETIC PEPTIDES AND PROTEASE INHIBITORS ON THE INTERACTION OF A HUMAN OVARIAN-CANCER CELL-LINE (NIH-OVCAR-3) WITH A RECONSTITUTED BASEMENT-MEMBRANE (MATRIGEL) SO INVASION & METASTASIS LA English DT Article DE INVITRO; INVASION; HUMAN OVARIAN CARCINOMA CELL ADHESION; PROTEASES; MATRIGEL ID AMINO-ACID SEQUENCE; TUMOR-CELLS; PLASMINOGEN-ACTIVATOR; INVITRO INVASIVENESS; EXTRACELLULAR-MATRIX; METASTATIC CELLS; CARCINOMA-CELLS; COLLAGENASE-IV; LAMININ; INVASION AB We have investigated the adhesive properties and invasiveness of cells of the human ovarian carcinoma line, NIH:OVCAR-3, in vitro. OVCAR-3 cells exhibited a similar rate of adhesion to all substrates tested including laminin, fibronectin, and collagens I and IV. The synthetic peptide YIGSR-NH2, which corresponds to an attachment site in laminin, inhibited the adhesion of the cells to laminin, but not to fibronectin. In contrast, a GRGDS-NH2 peptide blocked adhesion to fibronectin but not to laminin. OVCAR-3 cells invaded and formed branched colonies on Matrigel. Colony formation was retarded by both YIGSR-NH2 and GRGDS-NH2 peptides. Serine protease inhibitors and human recombinant TIMP, the tissue inhibitor of metalloproteases, inhibited ovarian tumor cell invasion while a synthetic collagenase IV inhibitor (SC-44463) had no effect. These studies suggest that metallo-proteases other than collagenase IV may be important for the invasive activity of ovarian cancer cells. It is possible that synthetic peptides with antiadhesive cellular activity and certain antiproteases could be used to control the progressive colonization and invasion of peritoneal surfaces by malignant ovarian cancer cells. C1 NIDR,DEV BIOL & ANOMALIES LAB,BETHESDA,MD 20892. NIA,GERONTOL RES CTR,BETHESDA,MD 20892. FOX CHASE CANC INST,PHILADELPHIA,PA 19111. NR 32 TC 31 Z9 32 U1 0 U2 2 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0251-1789 J9 INVAS METAST JI Invasion Metastasis PD MAR-APR PY 1991 VL 11 IS 2 BP 84 EP 92 PG 9 WC Oncology SC Oncology GA GH181 UT WOS:A1991GH18100004 PM 1917387 ER PT J AU OKAZAKI, K FURUNO, N WATANABE, N IKAWA, Y WOUDE, GFV SAGATA, N AF OKAZAKI, K FURUNO, N WATANABE, N IKAWA, Y WOUDE, GFV SAGATA, N TI CORRELATION BETWEEN PHYSIOLOGICAL AND TRANSFORMING ACTIVITIES OF THE C-MOS PROTOONCOGENE PRODUCT AND IDENTIFICATION OF AN ESSENTIAL MOS DOMAIN FOR THESE ACTIVITIES SO JAPANESE JOURNAL OF CANCER RESEARCH LA English DT Note DE C-MOS; PROTOONCOGENE; PHYSIOLOGICAL ACTIVITY; TRANSFORMING ACTIVITY; MOS; DOMAIN STRUCTURE ID MEIOTIC MATURATION; XENOPUS OOCYTES; EXPRESSION; HOMOLOG; EGGS; DNA AB Using Xenopus eggs and NIH3T3 cells as assay systems, we have compared the physiological (i.e., maturation-inducing and cleavage-arresting) and in vitro transforming activities of the c-mos genes from various species as well as their mutant genes. These analyses show that the three biological activities all depend upon the intrinsic protein kinase activity of Mos and correlate well with each other. Furthermore, our results demonstrate that a well conserved N-terminal 14-amino acid sequence of Mos, termed the Mos-box, is essential for all three activities. These results indicate that the in vitro transforming activity of Mos can be ascribed to the same kinase activity of Mos that exerts the physiological activities. C1 INST PHYS & CHEM RES,MOLEC ONCOL LAB,IBARAKI 305,JAPAN. TOKYO MED & DENT UNIV,DEPT BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN. NCI,BIONET RES INC,FREDERICK,MD 21701. RP OKAZAKI, K (reprint author), KURUME UNIV,INST LIFE SCI,DIV MOLEC GENET,2432-3 AIKAWA,KURUME,FUKUOKA 830,JAPAN. RI Okazaki, Kenji/H-4393-2012; Watanabe, Nobumoto/N-6959-2015 NR 18 TC 12 Z9 12 U1 0 U2 0 PU JAPANESE CANCER ASSOCIATION PI TOKYO PA EDITORIAL OFFICE 7TH FLOOR, JOHKOH BLDG 2-23-11, KOISHIKAWA, TOKYO 112, JAPAN SN 0910-5050 J9 JPN J CANCER RES JI Jpn. J. Cancer Res. PD MAR PY 1991 VL 82 IS 3 BP 250 EP 253 PG 4 WC Oncology SC Oncology GA FD053 UT WOS:A1991FD05300004 PM 1827090 ER PT J AU TAKANASHI, A YASUI, W YOSHIDA, K YOKOZAKI, H SAITO, D ABE, K URAKAMI, K MIKI, K TAHARA, E AF TAKANASHI, A YASUI, W YOSHIDA, K YOKOZAKI, H SAITO, D ABE, K URAKAMI, K MIKI, K TAHARA, E TI INHIBITORY EFFECT OF 8-CHLORO-CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE ON CELL-GROWTH OF GASTRIC-CARCINOMA CELL-LINES SO JAPANESE JOURNAL OF CANCER RESEARCH LA English DT Article DE GASTRIC CARCINOMA; CAMP-DEPENDENT PROTEIN KINASE; 8-CL-CAMP; TGF-ALPHA ID DEPENDENT PROTEIN-KINASE; CYCLIC-AMP ANALOGS; REGULATORY SUBUNIT; MOLECULAR-CLONING; BINDING PROTEIN; CANCER-CELLS; DNA; DIFFERENTIATION; EXPRESSION; GENE AB A cAMP analogue, 8-chloro-cAMP (8-Cl-cAMP), selectively binds to site 1 receptor of type II regulatory subunit (RII) of cAMP-dependent protein kinase. The effects of 8-Cl-cAMP on human gastric carcinoma cell lines were studied. Twenty mu-M 8-Cl-cAMP clearly inhibited cell growth in six cell lines (TMK-1, KATO-III, MKN-7, -28, -45, and -74) but not in MKN-1. Cell population in the G1 phase was increased in KATO III cells, which were most responsive to 8-Cl-cAMP, while cell cycle progression in TMK-1 and MKN-1 cells was apparently not influenced by 8-Cl-cAMP. The various changes induced by 8-Cl-cAMP were further analyzed in TMK-1 cells. Decrease of type I regulatory subunit (RI) of cAMP-dependent protein kinase and translocation of RII from cytosol to nucleus were induced by 8-Cl-cAMP treatment. 8-Cl-cAMP increased the level of cAMP-response element (CRE) binding protein in addition to inducing FOS mRNA, whose promoter contains CRE. 8-Cl-cAMP decreased the expression of mRNA for transforming growth factor-alpha (TGF-alpha), while the expression of epidermal growth factor receptor was not changed. Expression of HRAS and MYC mRNAs was slightly increased, whereas the amounts of HRAS and MYC proteins remained unchanged. Our results overall suggest that 8-Cl-cAMP might be useful tool for antitumor therapy of gastric cancers and that cell growth inhibition by 8-Cl-cAMP might account for the decrease of TGF-alpha expression by tumor cells. C1 HIROSHIMA UNIV,SCH MED,DEPT PATHOL 1,1-2-3 KASUMI,MINAMI KU,HIROSHIMA 734,JAPAN. TONEN CORP,FUNDAMENTAL RES LABS,CORP RES & DEV LAB,IRUMA,SAITAMA 354,JAPAN. NCI,TUMOR IMMUNOL & BIOL LAB,CELLULAR BIOCHEM SECT,BETHESDA,MD 20892. NATL CANC CTR,DEPT INTERNAL MED,CHUO KU,TOKYO 104,JAPAN. NATL MATSUDO HOSP,CHIBA 271,JAPAN. OI Yokozaki, Hiroshi/0000-0001-5276-3331 NR 29 TC 16 Z9 16 U1 0 U2 0 PU JAPANESE CANCER ASSOCIATION PI TOKYO PA EDITORIAL OFFICE 7TH FLOOR, JOHKOH BLDG 2-23-11, KOISHIKAWA, TOKYO 112, JAPAN SN 0910-5050 J9 JPN J CANCER RES JI Jpn. J. Cancer Res. PD MAR PY 1991 VL 82 IS 3 BP 325 EP 331 PG 7 WC Oncology SC Oncology GA FD053 UT WOS:A1991FD05300016 PM 1850725 ER PT J AU HIMENO, A SAAVEDRA, JM HAYASHIDA, M TSUJIMURA, T TATEISHI, T NAKANE, Y AF HIMENO, A SAAVEDRA, JM HAYASHIDA, M TSUJIMURA, T TATEISHI, T NAKANE, Y TI CHARACTERIZATION OF HUMAN PLATELET [I-125] R-DOI BINDING-SITES BY INVITRO AUTORADIOGRAPHY SO JAPANESE JOURNAL OF PSYCHIATRY AND NEUROLOGY LA English DT Article ID ACID DIETHYLAMIDE BINDING; RECEPTOR-BINDING; 5-HT2 RECEPTOR; TISSUE C1 NAGASAKI UNIV,SCH MED,DEPT NEUROPSYCHIAT,NAGASAKI 852,JAPAN. NIMH,CLIN SCI LAB,PHARMACOL SECT,BETHESDA,MD 20892. NR 7 TC 0 Z9 0 U1 0 U2 0 PU FOLIA PUBL SOC PI TOKYO PA ACADEMIC SOCIETIES BLDG 2-4-16 YAYOI, BUNKYO-KU, TOKYO 113, JAPAN SN 0912-2036 J9 JPN J PSYCHIAT NEUR PD MAR PY 1991 VL 45 IS 1 BP 115 EP 116 PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA FK289 UT WOS:A1991FK28900020 PM 1753453 ER PT J AU LUNDGREN, JD DAVEY, RT LUNDGREN, B MULLOL, J MAROM, Z LOGUN, C BARANIUK, J KALINER, MA SHELHAMER, JH AF LUNDGREN, JD DAVEY, RT LUNDGREN, B MULLOL, J MAROM, Z LOGUN, C BARANIUK, J KALINER, MA SHELHAMER, JH TI EOSINOPHIL CATIONIC PROTEIN STIMULATES AND MAJOR BASIC-PROTEIN INHIBITS AIRWAY MUCUS SECRETION SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Article ID ARACHIDONIC-ACID; ASTHMA; INVITRO; GRANULE; RELEASE; PURIFICATION; EPITHELIUM; NEUROTOXIN; DAMAGE; CELLS AB Possible roles of eosinophil (EO) products in modulating the release of mucus from airway explants were investigated. Cell- and membrane-free lysates from purified human EOs (1 to 20 x 10(5)) caused a dose-dependent release of respiratory glycoconjugates (RGC) from cultured feline tracheal explants. Crude extracts from isolated EO granules also stimulated RGC release, suggesting that a granular protein might be responsible. Three proteins derived from EO granules, EO-derived neurotoxin, EO cationic protein (ECP), and major basic protein (MBP) were separated by sequential sizing and affinity chromatography. ECP (0.025 to 25-mu-g/ml) caused a dose-dependent increase in RGC release from both feline and human airway explants and also stimulated the release of the serous cell-marker, lactoferrin, from human bronchial explants. EO-derived neurotoxin (0.025 to 50-mu-g/ml) failed to affect RGC release, whereas MBP (50-mu-g/ml) significantly inhibited RGC release from feline explants. Thus, ECP stimulates RGC and lactoferrin release from airway explants, whereas MBP inhibits RGC release. C1 NIAID,CTR CLIN,DEPT CRIT CARE MED,BETHESDA,MD 20892. NIAID,DIV PARASITOL,BETHESDA,MD 20892. NIAID,ALLERG DIS SECT,BETHESDA,MD 20892. MT SINAI MED CTR,NEW YORK,NY 10029. OI Lundgren, Jens/0000-0001-8901-7850 NR 32 TC 56 Z9 56 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD MAR PY 1991 VL 87 IS 3 BP 689 EP 698 DI 10.1016/0091-6749(91)90390-A PG 10 WC Allergy; Immunology SC Allergy; Immunology GA FB801 UT WOS:A1991FB80100014 PM 2005321 ER PT J AU MITCHELL, JM PAUL, BD WELCH, P CONE, EJ AF MITCHELL, JM PAUL, BD WELCH, P CONE, EJ TI FORENSIC DRUG-TESTING FOR OPIATES .2. METABOLISM AND EXCRETION RATE OF MORPHINE IN HUMANS AFTER MORPHINE ADMINISTRATION SO JOURNAL OF ANALYTICAL TOXICOLOGY LA English DT Article ID URINARY-EXCRETION; CODEINE; HEROIN AB Urine levels of free and total morphine were determined by GC/MS for four male subjects who received single doses of 20 mg of morphine sulfate intramuscularly. Peak concentrations were observed within 10 h for both conjugated and free morphine; thereafter, levels declined rapidly. Initially, free morphine represented from 25 to 34% of the total amount of morphine present, but this ratio declined after 12 h to an average of only 5.9% of total morphine. Free morphine accounted for an overall mean of 6.8% of the dose excreted in urine and conjugated morphine for 58.6%. The mean excretion half-life for free morphine was 6.6 h and for conjugated morphine was 8.2 h. The lower concentration and shorter half-life of free morphine resulted in a shorter detection time for free morphine versus total morphine at a 300-ng/mL cutoff. An equivalent detection time for free morphine was obtained when its cutoff was lowered to 25 ng/mL. The possibility that morphine is metabolized to codeine was unequivocally ruled out by the finding of an absence of codeine at or above the LOD of the GC/MS assay in all clinical specimens collected after morphine administration. C1 NIDA,ADDICT RES CTR,POB 5180,BALTIMORE,MD 21224. USN,DRUG SCREENING LAB,NORFOLK,VA 23511. NR 19 TC 30 Z9 32 U1 0 U2 7 PU PRESTON PUBLICATIONS INC PI NILES PA 7800 MERRIMAC AVE PO BOX 48312, NILES, IL 60648 SN 0146-4760 J9 J ANAL TOXICOL JI J. Anal. Toxicol. PD MAR-APR PY 1991 VL 15 IS 2 BP 49 EP 53 PG 5 WC Chemistry, Analytical; Toxicology SC Chemistry; Toxicology GA FC710 UT WOS:A1991FC71000001 PM 2051744 ER PT J AU GLADEN, BC WILLIAMS, J CHAPIN, RE AF GLADEN, BC WILLIAMS, J CHAPIN, RE TI ISSUES IN THE STATISTICAL-ANALYSIS OF SPERM MOTION DATA DERIVED FROM COMPUTER-ASSISTED SYSTEMS SO JOURNAL OF ANDROLOGY LA English DT Article DE SPERM MOTILITY; STATISTICS; COMPUTER-ASSISTED SEMEN ANALYSIS (CASA) ID SEMEN ANALYSIS; MOTILITY AB Computer-assisted sperm motion measurements present certain features that must be accounted for in statistical analyses. Some are specific to this type of data, whereas others are standard considerations. For example, the measurement of multiple sperm from individuals creates correlations that must be accounted for if each sperm's measurement is used, and unequal variances may arise that need to be addressed if an average measurement from the individual is used. Also, the limitations on the ranges of some measurements create discrepancies between observed and actual means and may make treatment-related effects more difficult to detect - a circumstance that has an impact on study design. When variables that are truly continuous are measured in a discrete fashion, odd effects may arise and care is needed. Other considerations, such as the shapes of distributions and correlations among various measurements, should also be examined. Attention to these details of statistical analysis are vital to proper interpretation of data. C1 NIEHS,DEV & REPROD TOXICOL GRP,RES TRIANGLE PK,NC 27709. RP GLADEN, BC (reprint author), NIEHS,STAT & BIOMATH BRANCH,MAIL DROP B3-02,POB 12233,RES TRIANGLE PK,NC 27709, USA. OI Chapin, Robert/0000-0002-5997-1261 NR 19 TC 17 Z9 17 U1 0 U2 0 PU AMER SOC ANDROLOGY, INC PI LAWRENCE PA C/O ALLEN PRESS, INC PO BOX 368, LAWRENCE, KS 66044 SN 0196-3635 J9 J ANDROL JI J. Androl. PD MAR-APR PY 1991 VL 12 IS 2 BP 89 EP 97 PG 9 WC Andrology SC Endocrinology & Metabolism GA FE979 UT WOS:A1991FE97900001 PM 2050584 ER PT J AU STOUT, V TORRESCABASSA, A MAURIZI, MR GUTNICK, D GOTTESMAN, S AF STOUT, V TORRESCABASSA, A MAURIZI, MR GUTNICK, D GOTTESMAN, S TI RCSA, AN UNSTABLE POSITIVE REGULATOR OF CAPSULAR POLYSACCHARIDE SYNTHESIS SO JOURNAL OF BACTERIOLOGY LA English DT Article ID ESCHERICHIA-COLI K-12; NUCLEOTIDE-SEQUENCE; CELL-DIVISION; ERWINIA-STEWARTII; BACILLUS-SUBTILIS; CLONING VECTORS; RNA-POLYMERASE; SULA PROTEIN; GENE; DNA AB RcsA is an unstable positive regulator required for the synthesis of colanic acid capsular polysaccharide in Escherichia coli. Degradation of the RcsA protein in vivo depends on the ATP-dependent Lon protease. DNA sequence analysis of the rcsA gene reveals a single open reading frame for a 23,500-Da highly basic protein (pl = 9.9), consistent with the observed size of the purified subunit of RcsA. The DNA and protein sequences are highly homologous to the rcsA gene and protein from Klebsiella pneumoniae and other species. The carboxy-terminal region of RcsA contains a possible helix-turn-helix DNA-binding motif that resembles sequences found at the carboxy terminus of RcsB, another positive regulator of capsule synthesis, and in several other transcriptional regulators including members of the LuxR family. rcsA62, a mutation in rcsA that leads to increased capsule synthesis, encodes a protein designated RcsA*, which differs from wild-type RcsA only in the replacement of Met-145 by valine. The RcsA* protein is subject to Lon-dependent degradation. The stability of wild-type RcsA in vivo is increased by multicopy RcsB. Conversely, RcsA is degraded more rapidly in rcsB mutant hosts than in wild-type hosts. These results suggest that RcsA and RcsB interact in vivo and are consistent with genetic experiments that indicate an interaction between RcsA and RcsB. Based on these experiments, we propose a model for capsule regulation in which RcsA interacts directly with RcsB to promote transcription of the genes for capsule synthesis. C1 NCI,MOLEC BIOL LAB,BLDG 37,ROOM 4B03,BETHESDA,MD 20892. NR 59 TC 158 Z9 161 U1 0 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD MAR PY 1991 VL 173 IS 5 BP 1738 EP 1747 PG 10 WC Microbiology SC Microbiology GA EZ170 UT WOS:A1991EZ17000024 PM 1999391 ER PT J AU BROWN, S AF BROWN, S TI GENES FOR 7S RNAS CAN REPLACE THE GENE FOR 4.5S RNA IN GROWTH OF ESCHERICHIA-COLI SO JOURNAL OF BACTERIOLOGY LA English DT Note ID SIGNAL-RECOGNITION PARTICLE; CLONING VEHICLES; PROMOTER; CONSTRUCTION; TRANSLATION; SEQUENCE AB 4.5S RNAs of eubacteria and 7S RNAs of archaebacteria and eukaryotes exist in a hairpin conformation. The apex of this hairpin displays structural and sequence similarities among both 4.5S and 7S RNAs. Furthermore, a hyphenated sequence of 16 nucleotides is conserved in all eubacterial 4.5S RNAs examined. In this article I report that 7S RNAs that contain this 16-nucleotide sequence are able to replace 4.5S RNA and permit growth of Escherichia coli. RP BROWN, S (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,CHROMOSOME BIOL LAB,FREDERICK,MD 21702, USA. OI Brown, Stanley/0000-0002-8453-8144 FU NCI NIH HHS [N01-CO-74101] NR 26 TC 19 Z9 19 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD MAR PY 1991 VL 173 IS 5 BP 1835 EP 1837 PG 3 WC Microbiology SC Microbiology GA EZ170 UT WOS:A1991EZ17000040 PM 1999396 ER PT J AU GARCIA, GE STADTMAN, TC AF GARCIA, GE STADTMAN, TC TI SELENOPROTEIN-A COMPONENT OF THE GLYCINE REDUCTASE COMPLEX FROM CLOSTRIDIUM-PURINOLYTICUM - NUCLEOTIDE-SEQUENCE OF THE GENE SHOWS THAT SELENOCYSTEINE IS ENCODED BY UGA SO JOURNAL OF BACTERIOLOGY LA English DT Article ID FORMATE DEHYDROGENASE; PROTEIN-COMPONENTS; ESCHERICHIA-COLI; PURIFICATION; SELENIUM; DNA; IDENTIFICATION; HOMOLOGY; CODON AB The gene encoding the selenoprotein A component of glycine reductase was isolated from Clostridium purinolyticum. The nucleotide sequence of this gene (grdA) was determined. The opal termination codon (TGA) was found in-frame at the position corresponding to the location of the selenocysteine residue in the gene product. A comparison of the nucleotide sequences and secondary mRNA structures corresponding to the selenoprotein A gene and the fdhF gene of Escherichia coli formate dehydrogenase shows that there is a similar potential for regulation of the specific insertion of selenocysteine at the UGA codon. RP GARCIA, GE (reprint author), NHLBI,BIOCHEM LAB,BETHESDA,MD 20892, USA. NR 27 TC 35 Z9 38 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD MAR PY 1991 VL 173 IS 6 BP 2093 EP 2098 PG 6 WC Microbiology SC Microbiology GA FB988 UT WOS:A1991FB98800035 PM 1825826 ER PT J AU KAWAMOTO, S ADELSTEIN, RS AF KAWAMOTO, S ADELSTEIN, RS TI CHICKEN NONMUSCLE MYOSIN HEAVY-CHAINS - DIFFERENTIAL EXPRESSION OF 2 MESSENGER-RNAS AND EVIDENCE FOR 2 DIFFERENT POLYPEPTIDES SO JOURNAL OF CELL BIOLOGY LA English DT Article ID SMOOTH-MUSCLE CELLS; 3T3 CELLS; MULTIGENE FAMILY; DNA-SEQUENCE; C-FOS; GENE; IDENTIFICATION; CLONING; PURIFICATION; MOTILITY AB Two different mRNAs encoding two different nonmuscle myosin heavy chains (MHCs) of approximately 200 kD have been identified in chicken nonmuscle cells, in agreement with the results of Katsuragawa et al. (Katsuragawa, Y., M. Yanagisawa, A. Inoue, and T. Masaki. 1989. Eur. J. Biochem. 184:611-616). In this paper, we quantitate the content of mRNA encoding the two MHCs in a number of different tissues using RNA blot analysis with two specific oligonucleotide probes. Our results show that the relative content of mRNA encoding MHC-A and MHC-B differs in a tissue-dependent manner. Thus the ratio of mRNA encoding MHC-A versus MHC-B varies from > 9:1 in spleen and intestinal epithelial cells, to 6:4 in kidney and 2:8 in brain. The effect of serum on MHC mRNA expression was studied in serum-starved cultures of chick embryo fibroblasts. Serum stimulation results in a threefold increase in the mRNA encoding MHC-A and a threefold decrease in mRNA encoding MHC-B. Using SDS polyacrylamide gels, we have separated two nonmuscle MHC isoforms (198 and 196 kD) that can be distinguished from each other by two-dimensional peptide mapping of chymotryptic digests. We provide preliminary evidence that the MHC-A mRNA encodes the 196-kD polypeptide and that the MHC-B mRNA encodes the 198-kD polypeptide. RP KAWAMOTO, S (reprint author), NHLBI,MOLEC CARDIOL LAB,BETHESDA,MD 20892, USA. NR 39 TC 192 Z9 193 U1 0 U2 1 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9525 J9 J CELL BIOL JI J. Cell Biol. PD MAR PY 1991 VL 112 IS 5 BP 915 EP 924 DI 10.1083/jcb.112.5.915 PG 10 WC Cell Biology SC Cell Biology GA EZ876 UT WOS:A1991EZ87600013 PM 1999462 ER PT J AU SHEN, DW LU, YG CHIN, KV PASTAN, I GOTTESMAN, MM AF SHEN, DW LU, YG CHIN, KV PASTAN, I GOTTESMAN, MM TI HUMAN HEPATOCELLULAR-CARCINOMA CELL-LINES EXHIBIT MULTIDRUG RESISTANCE UNRELATED TO MDR1 GENE-EXPRESSION SO JOURNAL OF CELL SCIENCE LA English DT Article DE P-GLYCOPROTEIN; HEPATOMA CELLS; DOXORUBICIN; CISPLATINUM ID INTRINSIC DRUG-RESISTANCE; RAT-LIVER; CANCER; OVEREXPRESSION; TRANSFERASE; RNA AB Multidrug resistance of human cancer cells may result from expression of P-glycoprotein, the product of the MDR1 gene, acting as an energy-dependent drug efflux pump. However, direct evidence that expression of the MDR1 gene contributes to the multidrug resistance of human liver carcinomas has not been established. In this study, we tested five cell lines derived from human hepatocellular carcinomas for sensitivity to a variety of drugs used widely as anticancer agents: these included vinblastine, doxorubicin, actinomycin D, mitomycin C, 5-fluorouracil, 6-mercaptopurine, melphalan, methotrexate, cis-platinum and etoposide (VP-16). All five hepatoma cell lines were resistant at different levels to these chemicals compared to human KB cells. Although it has been demonstrated that resistance to vinblastine, colchicine, doxorubicin and actinomycin D in human multidrug-resistant cells is associated with overexpression of P-glycoprotein, very little expression of P-glycoprotein was found in these human hepatoma cells. Neither verapamil nor quinidine, inhibitors of the drug efflux pump, were able to overcome multidrug resistance in hepatoma cells. These results indicate that the multidrug resistance phenotype in human hepatocellular carcinoma cells cannot be attributed to expression of the MDR1 gene, but that novel mechanisms may account for the resistance of these cancer cells. C1 NCI,CELL BIOL LAB,BLDG 37,ROOM 2E18,BETHESDA,MD 20892. NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. RI Chin, Khew-Voon/F-2670-2013 NR 32 TC 56 Z9 56 U1 0 U2 2 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL SN 0021-9533 J9 J CELL SCI JI J. Cell Sci. PD MAR PY 1991 VL 98 BP 317 EP 322 PN 3 PG 6 WC Cell Biology SC Cell Biology GA FD318 UT WOS:A1991FD31800008 PM 1676033 ER PT J AU FREED, WJ POLTORAK, M TAKASHIMA, H LAMARCA, ME GINNS, EI AF FREED, WJ POLTORAK, M TAKASHIMA, H LAMARCA, ME GINNS, EI TI BRAIN GRAFTS AND PARKINSONS-DISEASE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Article DE TISSUE TRANSPLANTATION; CATECHOLAMINES; DOPAMINE; L-DOPA; GENETIC ENGINEERING ID ADRENAL-MEDULLA GRAFTS; SUBSTANTIA NIGRA TRANSPLANTS; TYROSINE-HYDROXYLASE GENE; DENERVATED STRIATUM; TISSUE TRANSPLANTATION; ROTATIONAL BEHAVIOR; DOPAMINE AGONISTS; CHROMAFFIN TISSUE; CELL-SUSPENSIONS; CAUDATE-NUCLEUS AB In animal models, grafts derived from several different tissues, principally fetal substantia nigra and adrenal medulla from young adults, have been found to be effective in alleviating some of the manifestations of lesions of the substantia nigra. It has been suggested that these grafts function by diffusely secreting dopamine, by exerting trophic effects on the host brain, or by producing a new innervation of the host corpus striatum. Evidence for each of these modes of action is briefly reviewed. Several brain tissue transplantation techniques have been described. Each of these techniques has significant limitations in animal models. The significance of these limitations for human application is described, and possibilities for improving the efficacy of brain tissue transplantation in animal models and for human application are discussed. C1 NIMH,INTRAMURAL RES PROGRAM,CLIN NEUROSCI BRANCH,WASHINGTON,DC 20032. NIH,INTRAMURAL RES PROGRAM,BETHESDA,MD 20892. RP FREED, WJ (reprint author), NIMH,CTR NEUROSCI,INTRAMURAL RES PROGRAM,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 57 TC 10 Z9 10 U1 1 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR PY 1991 VL 45 IS 3 BP 261 EP 267 DI 10.1002/jcb.240450307 PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FC725 UT WOS:A1991FC72500006 PM 2066376 ER PT J AU GELLER, HM QUINONESJENAB, V POLTORAK, M FREED, WJ AF GELLER, HM QUINONESJENAB, V POLTORAK, M FREED, WJ TI APPLICATIONS OF IMMORTALIZED CELLS IN BASIC AND CLINICAL NEUROLOGY SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Article DE IMMORTALIZATION; CELL LINES; ASTROCYTES; RETROVIRUSES; GRAFTS; TEMPERATURE SENSITIVE ID NERVE GROWTH-FACTOR; HYPOTHALAMIC NEUROSECRETORY CELLS; PRESENTING ASTROCYTIC CHARACTERS; PC12 PHEOCHROMOCYTOMA CELLS; MURINE RETROVIRUS; MELANOMA-CELLS; MOUSE STRIATUM; RAT-BRAIN; LINES; DIFFERENTIATION AB Immortalized cell lines can serve as model systems for studies of neuronal development and restoration of function in models of neurological disease. Cell lines which result from spontaneous or experimentally-induced tumors have been used for these purposes. More recently, the techniques of genetic engineering have resulted in the production of cell lines with specific desired characteristics. This has been accomplished by insertion of a desired gene into a pre-existing immortal cell or by immortalizing primary cells. The production of immortal cell lines using temperature-sensitive immortalizing genes offers an additional method of controlling gene expression, and thereby controlling cell proliferation and differentiation. In the nervous system, these techniques have produced immortal cell lines with neuronal and glial properties. C1 RUTGERS STATE UNIV,GRAD SCH,PISCATAWAY,NJ 08854. ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,WASHINGTON,DC 20032. RP GELLER, HM (reprint author), UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT PHARMACOL,675 HOES LANE,PISCATAWAY,NJ 08854, USA. OI Geller, Herbert/0000-0002-7048-6144 FU NINDS NIH HHS [P01 NS 21469] NR 44 TC 13 Z9 14 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR PY 1991 VL 45 IS 3 BP 279 EP 283 DI 10.1002/jcb.240450310 PG 5 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA FC725 UT WOS:A1991FC72500009 PM 2066379 ER PT J AU TASHIRO, K SEPHEL, GC GREATOREX, D SASAKI, M SHIRASHI, N MARTIN, GR KLEINMAN, HK YAMADA, Y AF TASHIRO, K SEPHEL, GC GREATOREX, D SASAKI, M SHIRASHI, N MARTIN, GR KLEINMAN, HK YAMADA, Y TI THE RGD CONTAINING SITE OF THE MOUSE LAMININ A CHAIN IS ACTIVE FOR CELL ATTACHMENT, SPREADING, MIGRATION AND NEURITE OUTGROWTH SO JOURNAL OF CELLULAR PHYSIOLOGY LA English DT Article ID SYNTHETIC PEPTIDE; A-CHAIN; FIBRONECTIN PROMOTE; ENDOTHELIAL-CELLS; HEPARIN-BINDING; IV COLLAGEN; RECEPTOR; ADHESION; INTEGRIN; INVITRO AB The laminin A chain has been sequenced by cDNA cloning and was found to contain an RGD sequence. Synthetic peptides containing the RGD sequence and flanking amino acids were active in mediating cell adhesion, spreading, migration, and neurite outgrowth. Furthermore, endothelial cell attachment to a laminin substrate was inhibited by an RGD-containing synthetic peptide. Antisera against the integrin (fibronectin) receptor, and monoclonal antibody to the integrin, VLA-6, inhibited cell interaction with laminin, as well as with peptides containing an RGD sequence. These results suggest that the RGD containing site of laminin is active and interacts with the integrin family of receptors in certain cells. C1 NIDR,DEV BIOL & ANOMALIES LAB,BETHESDA,MD 20892. NR 57 TC 98 Z9 98 U1 1 U2 8 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0021-9541 J9 J CELL PHYSIOL JI J. Cell. Physiol. PD MAR PY 1991 VL 146 IS 3 BP 451 EP 459 DI 10.1002/jcp.1041460316 PG 9 WC Cell Biology; Physiology SC Cell Biology; Physiology GA FH224 UT WOS:A1991FH22400015 PM 2022699 ER PT J AU HOLDEN, JE MORI, K DIENEL, GA CRUZ, NF NELSON, T SOKOLOFF, L AF HOLDEN, JE MORI, K DIENEL, GA CRUZ, NF NELSON, T SOKOLOFF, L TI MODELING THE DEPENDENCE OF HEXOSE DISTRIBUTION VOLUMES IN BRAIN ON PLASMA-GLUCOSE CONCENTRATION - IMPLICATIONS FOR ESTIMATION OF THE LOCAL 2-DEOXYGLUCOSE LUMPED CONSTANT SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article DE HEXOSE DISTRIBUTION SPACES; LOCAL LUMPED CONSTANT; MATHEMATICAL MODELING; PLASMA GLUCOSE CONCENTRATION ID RAT-BRAIN; TRANSPORT; PHOSPHORYLATION; BARRIER; KINETICS; ANALOGS AB The steady-state distribution volumes of glucose, 3-O-methylglucose, and 2-deoxyglucose (2DG) are known to change as the concentration of glucose in plasma ranges from hypo- to hyperglycemic values. Model estimates of the three distribution volumes were compared with distribution volume values experimentally measured in the brains of conscious rats as the concentration of glucose in plasma was varied from 2 to 28 mM. The dependence on plasma glucose concentration of the 2DG lumped constant, the factor that relates the phosphorylation rate of 2DG to the net rate of glucose utilization at unit specific radioactivity in the plasma, had been determined previously in separate series of experiments. The model was extended to incorporate this dependence of the lumped constant. In the model both the transport and the phosphorylation barriers were assumed to be single and saturable. The values of their respective half-saturation concentrations and the ratio of the two maximum velocities for glucose were assumed to be invariant over the entire range of plasma glucose concentration. Good agreement between measured and estimated values for the distribution volumes and the lumped constant was attained over the full range of plasma glucose concentration. The model estimates reflected the progressive transport limitation of the brain glucose content as plasma glucose levels were reduced to hypoglycemic values. The results also indicated that these changes should be evident in the time course of 2DG in brain following administration by bolus or continuous infusion, and thus that indexes of local lumped constant change could be derived from time course data. C1 NIMH,CEREBRAL METAB LAB,BETHESDA,MD 20892. RP HOLDEN, JE (reprint author), UNIV WISCONSIN,CTR MED SCI 1530,DEPT MED PHYS,1300 UNIV AVE,MADISON,WI 53706, USA. NR 30 TC 36 Z9 36 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD MAR PY 1991 VL 11 IS 2 BP 171 EP 182 PG 12 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA EZ346 UT WOS:A1991EZ34600001 PM 1997495 ER PT J AU SAWADA, Y KAWAI, R MCMANAWAY, M OTSUKI, H RICE, KC PATLAK, CS BLASBERG, RG AF SAWADA, Y KAWAI, R MCMANAWAY, M OTSUKI, H RICE, KC PATLAK, CS BLASBERG, RG TI KINETIC-ANALYSIS OF TRANSPORT AND OPIOID RECEPTOR-BINDING OF [H-3] ( - )-CYCLOFOXY IN RAT-BRAIN INVIVO - IMPLICATIONS FOR HUMAN STUDIES SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article DE CYCLOFOXY; OPIATE ANTAGONIST; OPIATE RECEPTOR; BBB-TRANSPORT; NONSPECIFIC BINDING; KD; BMAX; INVIVO; BRAIN ID POSITRON EMISSION TOMOGRAPHY; LIVING HUMAN-BRAIN; OPIATE-RECEPTOR; AUTORADIOGRAPHIC LOCALIZATION; BLOOD-FLOW; ANTAGONIST; CYCLOFOXY; VISUALIZATION; DOPAMINE; LIGANDS AB [H-3]Cyclofoxy (CF: 17-cyclopropylmethyl-3,14-dihydroxy-4,5-alpha-epoxy-6-beta-fluoromorphinan) is an opioid antagonist with affinity to both mu and kappa subtypes that was synthesized for quantitative evaluation of opioid receptor binding in vivo. Two sets of experiments in rats were analyzed. The first involved determining the metabolite-corrected blood concentration and tissue distribution of CF in brain 1 to 60 min after i.v. bolus injection. The second involved measuring brain washout for 15 to 120 s following intracarotid artery injection of CF. A physiologically based model (Sawada et al., 1990a) and a classical compartmental pharmacokinetic model (Wong et al., 1986a) were compared. The models included different assumptions for transport across the blood-brain barrier (BBB); estimates of nonspecific tissue binding and specific binding to a single opiate receptor site were found to be essentially the same with both models. The nonspecific binding equilibrium constant varied modestly in different brain structures (K(eq) = 3-9), whereas the binding potential (BP) varied over a much broader range (BP = 0.6-32). In vivo estimates of the opioid receptor dissociation constant were similar for different brain structures (K(D) = 2.1-5.2 nM), whereas the apparent receptor density (B(max) varied between 1 (cerebellum) and 78 (thalamus) pmol/g of brain. The receptor dissociation rate constants in cerebrum (k4 = 0.08-0.16 min-1; k(off) = 0.16-0.23 min-1) and brain vascular permeability (PS = 1.3-3.4 ml/min/g) are sufficient high to achieve equilibrium conditions within a reasonable period of time. Graphical analysis (Patlak and Blasberg, 1985) of the data is inappropriate due to the high tissue-loss rate constant (k(b = 0.03-0.07 min-1) for CF in brain. From these findings, CF should be a very useful opioid receptor ligand for the estimation of the receptor binding parameters in human subjects using [F-18]CF and positron emission tomography. C1 NIDDK,CTR CLIN,DEPT NUCL MED,MED CHEM LAB,BETHESDA,MD. NIMH,DIV BIOMETRY & APPL SCI,THEORET STAT & MATH BRANCH,BETHESDA,MD 20892. NR 63 TC 11 Z9 11 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD MAR PY 1991 VL 11 IS 2 BP 183 EP 203 PG 21 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA EZ346 UT WOS:A1991EZ34600002 PM 1847702 ER PT J AU CARSON, RE AF CARSON, RE TI PRECISION AND ACCURACY CONSIDERATIONS OF PHYSIOLOGICAL QUANTITATION IN PET SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article; Proceedings Paper CT WORKSHOP ON PET ( POSITRON EMISSION TOMOGRAPHY ) DATA ANALYSIS CY MAY 01-02, 1989 CL NEW YORK, NY DE MODELS; FUNCTIONAL IMAGING; NORMALIZATION ID CEREBRAL BLOOD-FLOW; POSITRON EMISSION TOMOGRAPHY; GLUCOSE METABOLIC-RATE; SHORT-LIVED ISOTOPES; COMPUTED-TOMOGRAPHY; RATE CONSTANTS; ERROR ANALYSIS; NORMAL VALUES; BRAIN; ISCHEMIA AB The ability to differentiate regional patterns of flow and metabolism between various patient populations depends upon the signal-to-noise characteristics of the data. The approach chosen for producing quantitative data will affect the detection sensitivity of a method. Methods based on mathematical models can reduce intersubject variability by accounting for factors unrelated to the physiological measure of interest, in particular, differences in the input function. However, errors in the model and in the implementation of a model-based method can increase variability compared to simpler, emprirical methods. Normalization of physiological measures can significantly reduce intersubject variation; however, interpretation of normalized results can be more complex. The advantages and disadvantages of various approaches for physiological quantitation are considered. RP CARSON, RE (reprint author), NIH,DEPT NUCL MED,BLDG 10,ROOM 1C-401,BETHESDA,MD 20892, USA. RI Carson, Richard/H-3250-2011 OI Carson, Richard/0000-0002-9338-7966 NR 32 TC 30 Z9 30 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD MAR PY 1991 VL 11 IS 2 BP A45 EP A50 PG 6 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA EZ346 UT WOS:A1991EZ34600028 PM 1997487 ER PT J AU GRADY, CL AF GRADY, CL TI QUANTITATIVE COMPARISON OF MEASUREMENTS OF CEREBRAL GLUCOSE METABOLIC-RATE MADE WITH 2 POSITRON CAMERAS SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article; Proceedings Paper CT WORKSHOP ON PET ( POSITRON EMISSION TOMOGRAPHY ) DATA ANALYSIS CY MAY 01-02, 1989 CL NEW YORK, NY DE PET; CEREBRAL METABOLIC GLUCOSE RATE; SCANNERS; COMPARISON ID EMISSION COMPUTED-TOMOGRAPHY; ALZHEIMERS-DISEASE; DEMENTIA; ABNORMALITIES; HUNTINGTONS AB The rapid progress in PET technology has created the dilemma of how to compare data from old and new tomographs. We examined cerebral metabolic data from two scanners, with different spatial resolutions and methods of attenuation correction, to see if metabolic values from the lower-resolution tomograph (ECAT II) could be adjusted to make them comparable to data from the higher-resolution scanner (Scanditronix PC1024-7B). Nine subjects were scanned on both tomographs after a single injection of [F-18]2-fluoro-2-deoxy-D-glucose. Regional and lobar gray matter metabolic rates for glucose were obtained from comparable images from each scanner. Ratios of lobar to global gray matter metabolism also were calculated. Regression coefficients and percent differences were computed to compare ECAT II and PC1024 data. Two-thirds of the region pairs showed significant regressions, although percent differences were quite variable, with measures of glucose utilization ranging from 30 to 120% higher on the PC1024 compared to those from the ECAT II. Comparisons of lobar glucose rates between the two machines were less variable (50 to 80%), and ratios differed by only +/- 5% (except for the temporal ratios). Since there was no simple and consistent relationship between regional metabolic rates on the two tomographs, an overall adjustment of regional ECAT values for comparison to PC1024 values would be impossible. A region-by-region adjustment would be necessary. Lobar ratios are sufficiently similar that direct comparisons might be possible. RP GRADY, CL (reprint author), NIA,NEUROSCI LAB,BLDG 10,ROOM 6C414,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 21 TC 15 Z9 15 U1 3 U2 3 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD MAR PY 1991 VL 11 IS 2 BP A57 EP A63 PG 7 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA EZ346 UT WOS:A1991EZ34600030 PM 1997489 ER PT J AU HORWITZ, B AF HORWITZ, B TI FUNCTIONAL INTERACTIONS IN THE BRAIN - USE OF CORRELATIONS BETWEEN REGIONAL METABOLIC RATES SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article; Proceedings Paper CT WORKSHOP ON PET ( POSITRON EMISSION TOMOGRAPHY ) DATA ANALYSIS CY MAY 01-02, 1989 CL NEW YORK, NY DE BRAIN METABOLISM; CORRELATION MATRIX; DEOXYGLUCOSE; POSITRON EMISSION TOMOGRAPHY; SIMULATION ID CROSS-CORRELATION ANALYSIS; GLUCOSE USE CORRELATIONS; ALZHEIMERS-DISEASE; STRIATE CORTEX; DOWNS-SYNDROME; INTERCORRELATIONS; PATTERNS; ADULTS; AGE AB Correlation coefficients between pairs of regional metabolic rates have been used to study patterns of functional associations among brain regions in humans and animals. An overview is provided concerning the additional information about brain functioning this type of analysis yields. A computer simulation model is presented for the purpose of giving a partial validation for correlational analysis. The model generates a set of simulated metabolic data upon which correlational analysis is performed. Because the underlying pattern of functional couplings in the model is known, these simulations demonstrate that the correlation coefficient between normalized metabolic rates is proportional to the strength of the functional coupling constant and that correlational analysis yields information on regional involvement in neural systems not evident in the pattern of absolute metabolic values. RP HORWITZ, B (reprint author), NIA,NEUROSCI LAB,BLDG 10,ROOM 6C414,BETHESDA,MD 20892, USA. NR 39 TC 32 Z9 35 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD MAR PY 1991 VL 11 IS 2 BP A114 EP A120 PG 7 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA EZ346 UT WOS:A1991EZ34600038 PM 1997479 ER PT J AU KARP, JS DAUBEWITHERSPOON, ME MUEHLLEHNER, G AF KARP, JS DAUBEWITHERSPOON, ME MUEHLLEHNER, G TI FACTORS AFFECTING ACCURACY AND PRECISION IN PET VOLUME IMAGING SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article; Proceedings Paper CT WORKSHOP ON PET ( POSITRON EMISSION TOMOGRAPHY ) DATA ANALYSIS CY MAY 01-02, 1989 CL NEW YORK, NY DE POSITRON EMISSION TOMOGRAPHY; VOLUME IMAGING; AXIAL SAMPLING; SPATIAL RESOLUTION; SCATTERED RADIATION ID POSITRON EMISSION TOMOGRAPHY; RECONSTRUCTION; PERFORMANCE; DETECTOR; DESIGN AB Volume imaging positron emission tomographic (PET) scanners with no septa and a large axial acceptance angle offer several advantages over multiring PET scanners. A volume imaging scanner combines high sensitivity with fine axial sampling and spatial resolution. The fine axial sampling minimizes the partial volume effect, which affects the measured concentration of an object. Even if the size of an object is large compared to the slice spacing in a multiring scanner, significant variation in the concentration is measured as a function of the axial position of the object. With a volume imaging scanner, it is necessary to use a three-dimensional reconstruction algorithm in order to avoid variations in the axial resolution as a function of the distance from the center of the scanner. In addition, good energy resolution is needed in order to use a high energy threshold to reduce the coincident scattered radiation. C1 NIH,DEPT NUCL MED,BETHESDA,MD 20892. RP KARP, JS (reprint author), HOSP UNIV PENN,DEPT RADIOL,3400 SPRUCE ST,PHILADELPHIA,PA 19104, USA. NR 12 TC 11 Z9 11 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD MAR PY 1991 VL 11 IS 2 BP A38 EP A44 PG 7 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA EZ346 UT WOS:A1991EZ34600027 PM 1997486 ER PT J AU RAPOPORT, SI AF RAPOPORT, SI TI DISCUSSION OF PET WORKSHOP REPORTS, INCLUDING RECOMMENDATIONS OF PET DATA-ANALYSIS WORKING GROUP SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article; Proceedings Paper CT WORKSHOP ON PET ( POSITRON EMISSION TOMOGRAPHY ) DATA ANALYSIS CY MAY 01-02, 1989 CL NEW YORK, NY DE POSITRON EMISSION TOMOGRAPHY; BRAIN METABOLISM; IMAGING ID GLUCOSE METABOLIC RATES; ALZHEIMERS-DISEASE; DOWNS-SYNDROME; BRAIN; INTERCORRELATIONS; ADULTS; AGE AB On May 1-2, 1989, a PET Data Analysis Working Group convened to consider positron emission tomography (PET) methodology and data analysis. The papers presented and the recommendations of the Group are reviewed. The Group recommended that a standard phantom of the human brain be used by different institutions to examine machine and data reconstruction PET variables. Interinstitutional comparisons could be aided by using a standard three-dimensional coordinate system. Deformations within individual diseased or atypical brains would require nonlinear as well as linear transformations to the standard space, using magnetic resonance images in register with the PET images. Methods for intersubject averaging of pixel-by-pixel or region-of-interest data, as well as appropriate statistical methods, need to be developed. PET data may first be exploratory and hypothesis-generating (with less stringent statistical theory), then later used to test hypotheses (with more stringent statistical criteria). Common databases, obtained by computer simulation models with known inherent structure, or directly by PET measurements on different groups, could be used to compare analytical and statistical methods among institutions. RP RAPOPORT, SI (reprint author), NIA,NEUROSCI LAB,BETHESDA,MD 20892, USA. NR 37 TC 18 Z9 18 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD MAR PY 1991 VL 11 IS 2 BP A140 EP A146 PG 7 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA EZ346 UT WOS:A1991EZ34600041 PM 1671781 ER PT J AU NAHIN, RL HUMPHREY, E HYLDEN, JLK AF NAHIN, RL HUMPHREY, E HYLDEN, JLK TI EVIDENCE FOR CALCITONIN GENE-RELATED PEPTIDE CONTACTS ON A POPULATION OF LAMINA-I PROJECTION NEURONS SO JOURNAL OF CHEMICAL NEUROANATOMY LA English DT Article DE SPINOPARABRACHIAL TRACT; WGA-HRP; CHOLERA TOXIN; IMMUNOCYTOCHEMISTRY; PAIN ID DORSAL HORN NEURONS; RAT SPINAL-CORD; PRIMARY SENSORY NEURONS; ROOT GANGLION-CELLS; SUBSTANCE-P; AFFERENT-FIBERS; IONTOPHORETIC APPLICATION; CONDUCTION-VELOCITY; RECEPTIVE-FIELDS; NOXIOUS STIMULI AB Using double-labeling techniques, we evaluated small diameter primary afferent input, as indicated by calcitonin gene-related peptide-immunoreactive varicosities, to a population of lamina I projection neurons in the rat lumbar spinal cord. About one third of the lamina I neurons labeled after injections of a retrograde tracer into the region surrounding the brachium conjunctivum received contacts from immunoreactive varicosities. Significantly fewer immunoreactive varicosities were in apposition to fusiform neurons than pyramidal or flattened neurons. A positive correlation was found between the size of the retrogradely labeled neuron and the number of contacts received. This study demonstrates that a known population of nociceptive lamina I neurons received direct input from presumed nociceptive primary afferents. RP NAHIN, RL (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 30,ROOM B-20,BETHESDA,MD 20892, USA. NR 55 TC 8 Z9 8 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0891-0618 J9 J CHEM NEUROANAT JI J. Chem. Neuroanat. PD MAR-APR PY 1991 VL 4 IS 2 BP 123 EP 129 DI 10.1016/0891-0618(91)90036-C PG 7 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA FH579 UT WOS:A1991FH57900004 PM 1711858 ER PT J AU WU, CYR XIA, TJ LIU, GS MCDIARMID, R AF WU, CYR XIA, TJ LIU, GS MCDIARMID, R TI STUDY OF HIGHER EXCITED RYDBERG STATES IN ETHYLENE SO JOURNAL OF CHEMICAL PHYSICS LA English DT Note ID PHOTOELECTRON-SPECTRA; SPECTROSCOPY; ASSIGNMENTS C1 UNIV SO CALIF,CTR SPACE SCI,LOS ANGELES,CA 90089. NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20205. RP WU, CYR (reprint author), UNIV SO CALIF,DEPT PHYS,LOS ANGELES,CA 90089, USA. NR 25 TC 7 Z9 7 U1 0 U2 2 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0021-9606 J9 J CHEM PHYS JI J. Chem. Phys. PD MAR 1 PY 1991 VL 94 IS 5 BP 4093 EP 4094 DI 10.1063/1.460639 PG 2 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA FA778 UT WOS:A1991FA77800096 ER PT J AU SWEDO, SE RAPOPORT, JL AF SWEDO, SE RAPOPORT, JL TI ANNOTATION - TRICHOTILLOMANIA SO JOURNAL OF CHILD PSYCHOLOGY AND PSYCHIATRY AND ALLIED DISCIPLINES LA English DT Note DE TRICHOTILLOMANIA; OBSESSIVE COMPULSIVE DISORDER; ETHOLOGY; CLOMIPRAMINE ID OBSESSIVE-COMPULSIVE DISORDER; HABIT-REVERSAL; CHILDHOOD; ADOLESCENTS; DEPRESSION; BEHAVIOR; THERAPY C1 DEPT HLTH & HUMAN SERV,BETHESDA,MD 20892. RP SWEDO, SE (reprint author), NIMH,CHILD PSYCHIAT BRANCH,BLDG 10,ROOM 6N240,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 60 TC 109 Z9 111 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0021-9630 J9 J CHILD PSYCHOL PSYC JI J. Child Psychol. Psychiatry Allied Discip. PD MAR PY 1991 VL 32 IS 3 BP 401 EP 409 DI 10.1111/j.1469-7610.1991.tb00319.x PG 9 WC Psychology, Developmental; Psychiatry; Psychology SC Psychology; Psychiatry GA FH180 UT WOS:A1991FH18000001 PM 2061361 ER PT J AU URBAN, RJ VELDHUIS, JD DUFAU, ML AF URBAN, RJ VELDHUIS, JD DUFAU, ML TI ESTROGEN REGULATES THE GONADOTROPIN-RELEASING HORMONE-STIMULATED SECRETION OF BIOLOGICALLY-ACTIVE LUTEINIZING-HORMONE SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID POSTMENOPAUSAL WOMEN; PITUITARY RESPONSIVENESS; RHESUS-MONKEY; ESTRADIOL; SERUM; LH; DECONVOLUTION; TESTOSTERONE; CLEARANCE; BIOASSAY AB Estrogen produces time-dependent bidirectional effects on the GnRH-stimulated release of immunoactive LH in various species. To examine estrogen's regulation of biologically active LH secretion in response to pulsatile stimulation by GnRH, we studied estrogen-deficient postmenopausal women basally and during treatment with diethlystilbesterol (DES; 1 mg, orally, daily). Basal and GnRH-stimulated plasma concentrations of bioactive LH were assayed by the in vitro rat interstitial cell testosterone bioassay. GnRH-promoted LH secretory bursts in response to two consecutive stimuli were quantitated by multiple parameter deconvolution analysis. Basal half-lives of LH averaged 171 +/- 17 min (immunoactive) and 223 +/- 10 min (bioactive). Analysis of variance revealed a significant decrease in mean basal plasma bioactive LH concentrations on days 10 and 30 of DES treatment. Mean serum immunoactive LH concentrations fell similarly. DES significantly increased the half-life of immunoactive LH (days 5 and 10), but did not change that of bioactive LH. GnRH self-priming of bioactive LH secretion (increased LH secretory peak 2 compared to peak 1) was demonstrated, with a maximal value on day 10 of DES treatment. In addition, the ratio of the mass of bioactive to immunoactive LH secreted in response to the first GnRH pulse was significantly enhanced by estrogen on day 5, whereas that after the second pulse of GnRH was significantly suppressed on day 30 of DES. The self-priming action of GnRH on bioactive LH release evident in the presence of oral DES was corroborated in a separate group of six women, who were treated for 30 days with 17-beta-estradiol via an intravaginally placed Silastic ring. In conclusion, we infer that estrogen exerts a highly selective effect on the gonadotroph secretory process, such that successive GnRH stimuli result in an increase in the maximal rate and mass of secretion of biologically active LH. C1 UNIV VIRGINIA, HLTH SCI CTR,INST BIODYNAM,DEPT INTERNAL MED, BOX 202, CHARLOTTESVILLE, VA 22908 USA. NICHHD, BETHESDA, MD 20892 USA. FU NCRR NIH HHS [RR-00847]; NIADDK NIH HHS [5-P60-AM-22125-05]; NICHD NIH HHS [1-KO4-HD-00634] NR 35 TC 35 Z9 35 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD MAR PY 1991 VL 72 IS 3 BP 660 EP 668 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FA523 UT WOS:A1991FA52300021 PM 1997520 ER PT J AU PARKER, RJ EASTMAN, A BOSTICKBRUTON, F REED, E AF PARKER, RJ EASTMAN, A BOSTICKBRUTON, F REED, E TI ACQUIRED CISPLATIN RESISTANCE IN HUMAN OVARIAN-CANCER CELLS IS ASSOCIATED WITH ENHANCED REPAIR OF CISPLATIN-DNA LESIONS AND REDUCED DRUG ACCUMULATION SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE CISPLATIN; OVARIAN CANCER; RESISTANCE; DNA REPAIR; ATOMIC ABSORPTION SPECTROMETRY; CHLORAMPHENICOL ACETYLTRANSFERASE; PRSVCAT ID ACETYLTRANSFERASE GENE-EXPRESSION; RAPID ASSAY; PLATINUM; ADDUCTS; CIS-DIAMMINEDICHLOROPLATINUM(II); QUANTITATION; REACTIVATION; CHEMOTHERAPY; FIBROBLASTS; MECHANISM AB Studies were undertaken to investigate acquired resistance to cisplatin in human ovarian cancer cells. The cell lines A2780 and A2780/CP70 were studied to assess their respective characteristics of drug accumulation and efflux, cytosolic inactivation of drug, and DNA repair. All experiments were performed using 1-h drug exposures. The A2780/CP70 cell line was 13-fold more resistant to cisplatin than A2780 cells. When studied at their respective IC50 doses, drug accumulation rates were similar for the two cell lines. However, the resistant cell line was twofold more efficient at effluxing drug, which was associated with reduced total drug accumulation for equivalent micromolar drug exposures. At equivalent levels of total cellular drug accumulation, the two cell lines formed the same levels of cisplatin-DNA damage, suggesting that cytosolic inactivation of drug does not contribute to the differential in resistance between these cell lines. Resistant cells were also twofold more efficient at repairing cisplatin-DNA lesions in cellular DNA and in transfected plasmid DNA. We conclude that in these paired cell lines, alterations in drug uptake/efflux and in DNA repair are the major contributing factors to acquired resistance to cisplatin. C1 NCI,CLIN PHARMACOL BRANCH,MED BRANCH,9000 ROCKVILLE PIKE,BLDG 10,RM 6N119,BETHESDA,MD 20892. DARTMOUTH COLL,HITCHCOCK MED CTR,DARTMOUTH MED SCH,DEPT PHARMACOL & TOXICOL,HANOVER,NH 03756. NR 30 TC 243 Z9 247 U1 0 U2 11 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAR PY 1991 VL 87 IS 3 BP 772 EP 777 DI 10.1172/JCI115080 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA FB171 UT WOS:A1991FB17100003 PM 1999494 ER PT J AU HSIAO, LL PELTONEN, J JAAKKOLA, S GRALNICK, H UITTO, J AF HSIAO, LL PELTONEN, J JAAKKOLA, S GRALNICK, H UITTO, J TI PLASTICITY OF INTEGRIN EXPRESSION BY NERVE-DERIVED CONNECTIVE-TISSUE CELLS - HUMAN SCHWANN-CELLS, PERINEURIAL CELLS, AND FIBROBLASTS EXPRESS MARKEDLY DIFFERENT PATTERNS OF BETA-1 INTEGRINS DURING NERVE DEVELOPMENT, NEOPLASIA, AND INVITRO SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE BASEMENT MEMBRANES; TYPE-IV COLLAGEN; FIBRONECTIN; NEUROFIBROMA; SCHWANNOMA; PERIPHERAL NERVE ID BASEMENT-MEMBRANE PROTEINS; EXTRACELLULAR-MATRIX; FIBRONECTIN RECEPTOR; TYPE-1 NEUROFIBROMATOSIS; SURFACE RECEPTORS; NEURITE OUTGROWTH; HUMAN-SKIN; LAMININ; COLLAGEN; ADHESION AB Strikingly selective expression patterns of beta-1, alpha-2, alpha-3, and alpha-5 integrin subunits were revealed in endoneurium, perineurium, and epineurium of fetal and adult human peripheral nerve by immunostaining with specific antibodies. The alpha-2 subunit was expressed only on Schwann cells both in fetal and adult nerve, whereas the alpha-3 epitopes were expressed exclusively in the adult tissue and were primarily present on perineurial cells. The alpha-5 epitopes were expressed only on the innermost cell layer of perineurium of fetal and adult nerve. The tumor cells within schwannomas and cutaneous neurofibromas expressed both alpha-2 and alpha-3 subunits, indicating that Schwann cells have the potential to express also the alpha-3 subunit in vivo. Cell cultures established from human fetal nerve and neurofibromas revealed expression of the alpha-2 and alpha-5 epitopes on Schwann cells, perineurial cells, and fibroblasts, whereas only Schwann cells contained the alpha-3 epitopes which were occasionally concentrated on the adjacent Schwann cells at cell-cell contacts. Our findings emphasize that nerve connective tissue cells change their profiles for expression of extracellular matrix receptors under conditions which have different regulatory control signals exerted by, for example, axons, humoral factors, or the extracellular matrix of the peripheral nerve. This plasticity may play an important role during nerve development and in neoplastic processes affecting the connective tissue compartments of peripheral nerve. C1 THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT DERMATOL, 1020 LOCUST ST, PHILADELPHIA, PA 19107 USA. THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT BIOCHEM & MOLEC BIOL, PHILADELPHIA, PA 19107 USA. THOMAS JEFFERSON UNIV, INST MOLEC MED, MOLEC DERMATOL SECT, PHILADELPHIA, PA 19107 USA. NIH, CTR CLIN, DEPT CLIN PATHOL, BETHESDA, MD 20892 USA. RI Peltonen, Juha/C-2817-2008; Peltonen, Sirkku/C-3133-2008 OI Peltonen, Juha/0000-0002-5732-4167; Peltonen, Sirkku/0000-0003-0990-1430 FU NIAMS NIH HHS [AR-35297, AR-38923, T32 AR-7561] NR 57 TC 35 Z9 35 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAR PY 1991 VL 87 IS 3 BP 811 EP 820 DI 10.1172/JCI115084 PG 10 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA FB171 UT WOS:A1991FB17100007 PM 1999496 ER PT J AU BRADY, LS WHITFIELD, HJ FOX, RJ GOLD, PW HERKENHAM, M AF BRADY, LS WHITFIELD, HJ FOX, RJ GOLD, PW HERKENHAM, M TI LONG-TERM ANTIDEPRESSANT ADMINISTRATION ALTERS CORTICOTROPIN-RELEASING HORMONE, TYROSINE-HYDROXYLASE, AND MINERALOCORTICOID RECEPTOR GENE-EXPRESSION IN RAT-BRAIN - THERAPEUTIC IMPLICATIONS SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE DEPRESSION; GLUCOCORTICOID RECEPTOR; IMIPRAMINE; MESSENGER RNA; PROOPIOMELANOCORTIN ID BIOCHEMICAL MANIFESTATIONS; DEPRESSED-PATIENTS; SECRETION; NEURONS; STRESS; NEUROBIOLOGY; SEQUENCE; CDNA AB Imipramine is the prototypic tricyclic antidepressant utilized in the treatment of major depression and exerts its therapeutic efficacy only after prolonged administration. We report a study of the effects of short-term (2 wk) and long-term (8 wk) administration of imipramine on the expression of central nervous system genes among those thought to be dysregulated in imipramine-responsive major depression. As assessed by in situ hybridization, 8 wk of daily imipramine treatment (5 mg/kg, i.p.) in rats decreased corticotropin-releasing hormone (CRH) mRNA levels by 37% in the paraventricular nucleus (PVN) of the hypothalamus and decreased tyrosine hydroxylase (TH) mRNA levels by 40% in the locus coeruleus (LC). These changes were associated with a 70% increase in mRNA levels of the hippocampal mineralocorticoid receptor (MR, type I) that is thought to play an important role in mediating the negative feedback effects of low levels of steroids on the hypothalamic-pituitary-adrenal (HPA) axis. Imipramine also decreased proopiomelanocortin (POMC) mRNA levels by 38% and glucocorticoid receptor (GR, type II) mRNA levels by 51% in the anterior pituitary. With the exception of a 20% decrease in TH mRNA in the LC after 2 wk of imipramine administration, none of these changes in gene expression were evident as a consequence of short-term administration of the drug. In the light of data that major depression is associated with an activation of brain CRH and LC-NE systems, the time-dependent effect of long-term imipramine administration on decreasing the gene expression of CRH in the hypothalamus and TH in the LC may be relevant to the therapeutic efficacy of this agent in depression. RP BRADY, LS (reprint author), NIMH,CLIN NEUROENDOCRINOL BRANCH,FUNCT NEUROANAT SECT,BLDG 36,ROOM 2D15,BETHESDA,MD 20892, USA. OI Herkenham, Miles/0000-0003-2228-4238 NR 29 TC 300 Z9 302 U1 1 U2 6 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAR PY 1991 VL 87 IS 3 BP 831 EP 837 DI 10.1172/JCI115086 PG 7 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA FB171 UT WOS:A1991FB17100009 PM 1671867 ER PT J AU LORTAT-JACOB, H KLEINMAN, HK GRIMAUD, JA AF LORTAT-JACOB, H KLEINMAN, HK GRIMAUD, JA TI HIGH-AFFINITY BINDING OF INTERFERON-GAMMA TO A BASEMENT-MEMBRANE COMPLEX (MATRIGEL) SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE BINDING SITE; EXTRACELLULAR MATRIX; LOCAL CONCENTRATION; HEPARAN SULFATE PROTEOGLYCAN ID HEPARAN-SULFATE PROTEOGLYCAN; EXTRACELLULAR-MATRIX; CELL-ADHESION; IMMUNE INTERFERON; TUMOR INVASION; GROWTH-FACTORS; LAMININ; IDENTIFICATION; RECEPTORS; MIGRATION AB Recently it was demonstrated that growth factors are bound to the extracellular matrix, and can regulate cell behavior. Using three different types of binding assays, we have examined the interaction of interferon-gamma with a basement membrane produced by the Engelbreth-Holm-Swarm tumor. Basement membrane was found to bind interferon-gamma in both a time- and concentration-dependent manner. Equilibrium binding analysis revealed a high-affinity site with a dissociation constant of 1.5 10(-9) M and a maximum binding capacity of 1.6 10(9) sites/mm2 of basement membrane. Competition studies show that the binding is inhibited by heparan sulfate, suggesting that basement membrane-heparan sulfate proteoglycan could be the binding site. This interaction was clearly confirmed by native polyacrylamide gel electrophoresis and dot-blot analysis with purified basement membrane molecules. Furthermore, the carboxy-terminal part of the interferon-gamma molecule contains an amino acid cluster, very closely related to a consensus sequence, present in more than 20 proteins known to bind sulfated glycosaminoglycans such as heparin. These data demonstrate a possible role of extracellular matrix components in storing cytokines and in modulating the cellular response to such factors. C1 INST PASTEUR, UNITE PATHOL CELLULAIRE,CNRS,URA 602,AVE T GARNIER, F-69365 LYONS 7, FRANCE. NIDR, DEV BIOL & ANOMALIES, BETHESDA, MD 20892 USA. NR 44 TC 98 Z9 98 U1 0 U2 0 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAR PY 1991 VL 87 IS 3 BP 878 EP 883 DI 10.1172/JCI115093 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA FB171 UT WOS:A1991FB17100016 PM 1900310 ER PT J AU MAI, UEH PEREZPEREZ, GI WAHL, LM WAHL, SM BLASER, MJ SMITH, PD AF MAI, UEH PEREZPEREZ, GI WAHL, LM WAHL, SM BLASER, MJ SMITH, PD TI SOLUBLE SURFACE-PROTEINS FROM HELICOBACTER-PYLORI ACTIVATE MONOCYTES MACROPHAGES BY LIPOPOLYSACCHARIDE-INDEPENDENT MECHANISM SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE HELICOBACTER-PYLORI; MONOCYTE MACROPHAGE; SURFACE PROTEINS; ACTIVATION; LIPOPOLYSACCHARIDE ID TUMOR NECROSIS FACTOR; PEPTIC-ULCER DISEASE; TOXIC-SHOCK-SYNDROME; CAMPYLOBACTER-LIKE ORGANISMS; A-ASSOCIATED PROTEINS; GASTRIC CAMPYLOBACTER; HUMAN INTERLEUKIN-1; ENDOTOXIN PROTEIN; EXPRESSION; MUCOSA AB The inflammatory lesions associated with Helicobacter pylori gastritis and duodenitis contain large numbers of mononuclear cells. The close proximity of H. pylori to gastric mucosa suggests that the organism interacts with mononuclear cells, thereby modulating the inflammatory response. To investigate the role of monocytes/macrophages in this response, we examined the effect of whole H. pylori bacteria, H. pylori surface proteins, and H. pylori lipopolysaccharide (LPS) on purified human monocytes. Whole H. pylori and the extracted LPS induced expression of the monocyte surface antigen HLA-DR and interleukin-2 receptors, production of the inflammatory cytokines interleukin 1 and tumor necrosis factor (peptide and messenger RNA), and secretion of the reactive oxygen intermediate superoxide anion. Since H. pylori in vivo does not invade mucosal tissue, we determined whether soluble constituents of the bacteria could activate monocytes. Soluble H. pylori surface proteins, which are enriched for urease and do not contain LPS, stimulated phenotypic, transcriptional, and functional changes consistent with highly activated monocytes. These findings indicate that H. pylori is capable of activating human monocytes by an LPS-independent as well as an LPS-dependent mechanism. H. pylori activation of resident lamina propria macrophages and monocytes trafficking through the mucosa, leading to the secretion of increased amounts of inflammatory cytokines and reactive oxygen intermediates, could play an important role in mediating the inflammatory response associated with H. pylori gastritis and duodenitis. C1 NIDR, IMMUNOL LAB,CELLULAR IMMUNOL SECT,BLDG 30,ROOM 322, BETHESDA, MD 20892 USA. VET ADM MED CTR, DEPT MED, DIV INFECT DIS, NASHVILLE, TN 37203 USA. VANDERBILT UNIV, MED CTR, SCH MED, NASHVILLE, TN 37232 USA. NR 58 TC 260 Z9 264 U1 0 U2 2 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAR PY 1991 VL 87 IS 3 BP 894 EP 900 DI 10.1172/JCI115095 PG 7 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA FB171 UT WOS:A1991FB17100018 PM 1847939 ER PT J AU MAEDA, R RAZ, I ZURLO, F SOMMERCORN, J AF MAEDA, R RAZ, I ZURLO, F SOMMERCORN, J TI ACTIVATION OF SKELETAL-MUSCLE CASEIN KINASE-II BY INSULIN IS NOT DIMINISHED IN SUBJECTS WITH INSULIN RESISTANCE SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE INSULIN SIGNAL TRANSDUCTION; PROTEIN PHOSPHORYLATION; PROTEIN KINASE ID EPIDERMAL GROWTH-FACTOR; PHOSPHATASE-ACTIVITY; PROTEIN-KINASE; PHOSPHOPROTEIN PHOSPHATASE; RECEPTOR; PHOSPHORYLATION; CELLS; STIMULATION; INVIVO AB Insulin resistance, which may precede the development of non-insulin-dependent diabetes mellitus in Pima Indians, appears to result from a postreceptor defect in signal transduction in skeletal muscle. To identify the putative postreceptor lesion responsible for insulin resistance in Pima Indians, we investigated the influence of insulin on the activity of casein kinase II (CKII) in skeletal muscle of seven insulin-sensitive, four insulin-resistant, nondiabetic, and five insulin-resistant diabetic Pima Indians during a 2 h hyperinsulinemic, euglycemic clamp. In sensitive subjects, CKII was transiently activated reaching a maximum over basal activity (42%) at 45 min before declining. CKII was also stimulated in resistant (19%) and diabetic (34%) subjects. Basal CKII activity in resistant subjects was 40% higher than in either sensitive or diabetic subjects, although the concentration of CKII protein, as determined by Western blotting, was equal among the three groups. Basal CKII activity was correlated with fasting plasma insulin concentrations, suggesting that the higher activity in resistant subjects resulted from insulin action. Extracts of muscle obtained from all three groups either before or after insulin administration were treated with immobilized alkaline phosphatase, which reduced and equalized CKII activity. These results suggest that insulin stimulates CKII activity in human skeletal muscle by a mechanism involving phosphorylation of either CKII or of an effector molecule, and support the idea that elevated basal activity in resistant subjects results from insulin action. It appears that the ability of insulin to activate CKII in skeletal muscle is not impaired in insulin-resistant Pima Indians, and that the biochemical lesion responsible for insulin resistance occurs either downstream from CKII or in a different pathway of insulin action. C1 NIDDKD,CLIN DIABET & NUTR SECT,4212 N 16TH ST,ROOM 541,PHOENIX,AZ 85016. NR 33 TC 21 Z9 21 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAR PY 1991 VL 87 IS 3 BP 1017 EP 1022 DI 10.1172/JCI115060 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA FB171 UT WOS:A1991FB17100034 PM 1999482 ER PT J AU OGUSHI, F HUBBARD, RC VOGELMEIER, C FELLS, GA CRYSTAL, RG AF OGUSHI, F HUBBARD, RC VOGELMEIER, C FELLS, GA CRYSTAL, RG TI RISK-FACTORS FOR EMPHYSEMA - CIGARETTE-SMOKING IS ASSOCIATED WITH A REDUCTION IN THE ASSOCIATION RATE-CONSTANT OF LUNG ALPHA-1-ANTITRYPSIN FOR NEUTROPHIL ELASTASE SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE PROTEASE; ANTIPROTEASE; ENZYME; OXIDATION ID HUMAN ALPHA-1-PROTEINASE INHIBITOR; BRONCHOALVEOLAR LAVAGE FLUIDS; LOWER RESPIRATORY-TRACT; OXIDATIVE INACTIVATION; ALVEOLAR MACROPHAGES; ALPHA1-ANTITRYPSIN DEFICIENCY; POLYMORPHONUCLEAR LEUKOCYTES; PROTEINASE-INHIBITORS; FUNCTIONAL-ACTIVITY; CONNECTIVE-TISSUE AB The increased risk of developing emphysema among individuals who smoke cigarettes and who have normal levels of alpha-1-antitrypsin (alpha-1AT) is hypothesized to result from a decrease in the antineutrophil elastase capacity of the lower respiratory tract alpha-1AT of smokers compared with nonsmokers. To evaluate this hypothesis we compared the time-dependent kinetics of the inhibition of neutrophil elastase by lung alpha-1AT from healthy, young cigarette smokers (n = 8) and nonsmokers (n = 12). Alpha-1-antitrypsin was purified from lavage fluid using affinity and molecular sieve chromatography, and the association rate constant (k assoc) for neutrophil elastase quantified. The k assoc of smoker plasma alpha-1AT (9.5 +/- 0.5 X 10(6) M-1s-1) was similar to that of nonsmoker plasma (9.3 +/- 0.7 X 10(6) M-1s-1, P > 0.5). In marked contrast, the k assoc of smoker lower respiratory tract alpha-1AT was significantly lower than that of nonsmoker alpha-1AT (6.5 +/- 0.4 X 10(6) M-1s-1 vs. 8.1 +/- 0.5 X 10(6) M-1s-1, P < 0.01). Furthermore, the smoker lower respiratory tract alpha-1AT k assoc was significantly less than that of autologous plasma (P < 0.01). When considered in the context of the concentration of alpha-1AT in the lower respiratory tract epithelial lining fluid, the inhibition time for neutrophil elastase of smoker lung alpha-1AT was twofold greater than that of nonsmoker lung alpha-1AT (smoker: 0.34 +/- 0.05 s vs. nonsmoker: 0.17 +/- 0.05 s, P < 0.01). Consequently, for concentrations of alpha-1AT in the lower respiratory tract it takes twice as long for an equivalent amount of neutrophil elastase to be inhibited in the smoker's lung compared with the nonsmoker's lung. These observations support the concept that cigarette smoking is associated with a decrease in the lower respiratory tract neutrophil elastase inhibitory capacity, thus increasing the vulnerability of the lung to elastolytic destruction and thereby increasing the risk for the development of emphysema. C1 NHLBI,PULM BRANCH,BLDG 10,ROOM 6DO3,BETHESDA,MD 20892. NR 64 TC 79 Z9 79 U1 0 U2 2 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAR PY 1991 VL 87 IS 3 BP 1060 EP 1065 DI 10.1172/JCI115066 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA FB171 UT WOS:A1991FB17100040 PM 1999486 ER PT J AU BRANDES, ME ALLEN, JB OGAWA, Y WAHL, SM AF BRANDES, ME ALLEN, JB OGAWA, Y WAHL, SM TI TRANSFORMING GROWTH-FACTOR BETA-1 SUPPRESSES ACUTE AND CHRONIC ARTHRITIS IN EXPERIMENTAL-ANIMALS SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Note DE CYTOKINE; INFLAMMATION; IMMUNOSUPPRESSION; LEUKOCYTOSIS; LEUKOCYTE ID CELLS; PROLIFERATION; FACTOR-BETA-1; RAT; INHIBITOR; CULTURES; BONE AB Systemic administration of the cytokine, TGF-beta-1, profoundly antagonized the development of polyarthritis in susceptible rats. TGF-beta-1 administration (1 or 5-mu-g/animal), initiated one day before an arthritogenic dose of streptococcal cell wall (SCW) fragments, virtually eliminated the joint swelling and distortion typically observed during both the acute phase (articular index, AI = 2.5 vs. 11; P < 0.025) and the chronic phase (AI = 0 vs. 12.5) of the disease. Moreover, TGF-beta-1 suppressed the evolution of arthritis even when administration was begun after the acute phase of the disease. Histopathological examination of the joint revealed the systemic TGF-beta-1 treatment greatly reduced inflammatory cell infiltration, pannus formation, and joint erosion. Consistent with the inhibition of inflammatory cell recruitment into the synovium, TGF-beta-1 reversed the leukocytosis associated with the chronic phase of the arthritis. Control animals subjected to the same TGF-beta-1 dosing regimen displayed no discernable immunosuppressive or toxic effects even after 4 wk of treatment. These observations not only provide insight into the immunoregulatory effects of TGF-beta, but also implicate this cytokine as a potentially important therapeutic agent. C1 COLLAGEN CORP,CELTRIX LABS,PALO ALTO,CA 94303. RP BRANDES, ME (reprint author), NIDR,IMMUNOL LAB,CELL IMMUNOL SECT,BLDG 30,ROOM 329,BETHESDA,MD 20892, USA. NR 35 TC 212 Z9 213 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAR PY 1991 VL 87 IS 3 BP 1108 EP 1113 DI 10.1172/JCI115073 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA FB171 UT WOS:A1991FB17100047 PM 1999490 ER PT J AU WARD, RL MCNEAL, MM CLEMENS, JD SACK, DA RAO, M HUDA, N GREEN, KY KAPIKIAN, AZ COULSON, BS BISHOP, RF GREENBERG, HB GERNA, G SCHIFF, GM AF WARD, RL MCNEAL, MM CLEMENS, JD SACK, DA RAO, M HUDA, N GREEN, KY KAPIKIAN, AZ COULSON, BS BISHOP, RF GREENBERG, HB GERNA, G SCHIFF, GM TI REACTIVITIES OF SEROTYPING MONOCLONAL-ANTIBODIES WITH CULTURE-ADAPTED HUMAN ROTAVIRUSES SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID LINKED IMMUNOSORBENT-ASSAY; RHESUS ROTAVIRUS; NEUTRALIZATION EPITOPES; ENZYME-IMMUNOASSAY; VENEZUELAN INFANTS; CHILDREN; EPIDEMIOLOGY; SUBGROUP; DISTRIBUTIONS; PROTECTION AB Rotaviruses collected in Bangladesh during 1985 to 1986 were culture adapted and used in a comparative serotyping study with three groups of monoclonal antibodies, all of which reacted with the major neutralization protein (VP7) of serotype 1, 2, 3, or 4. The goals were to determine which monoclonal antibodies most accurately predicted the serotype and why large variations in serotyping efficiencies have occurred with these monoclonal antibodies in previous studies. The 143 rotavirus isolates used in this study belonged to 69 different electropherotypes; and 44, 23, 21, and 55 isolates were identified as serotype 1 through 4, respectively, by neutralization with serotype-specific hyperimmune antisera. Serotyping specificity by enzyme-linked immunosorbent assay with monoclonal antibodies was 100% consistent with results found by neutralization with polyclonal antisera, but large differences were observed in the sensitivities of the different monoclonal antibodies. Monoclonal antibodies 5E8 (serotype 1), 1C10 (serotype 2), 159 (serotype 3), RV3:1 (serotype 3), ST-3:1 (serotype 4), and ST-2G7 (serotype 4) reacted with all the isolates of the corresponding serotype for which there were sufficient infectious particles. Monoclonal antibody 2F1 (serotype 2) was much less sensitive and reacted with only five serotype 2 isolates, but these were among those with the highest titers. Monoclonal antibodies RV4:2 (serotype 1), KU6BG (serotype 1), RV5:3 (serotype 2), and S2-2G10 (serotype 2), on the other hand, failed to react with between one and three isolates of the corresponding serotypes which had high titers, apparently because of epitope changes in these isolates. Effects of epitope variation were, however, most apparent with monoclonal antibodies 2C9 (serotype 1) and YO-1E2 (serotype 3), which reacted with one and no isolates of the corresponding serotypes, respectively. Cross-neutralization of escape mutants indicated that the serotype 1 monoclonal antibodies 5E8, 2C9, and RV4:2 reacted with different but probably overlapping epitopes, as did serotype 2 monoclonal antibodies 2F1, 1C10, and RV5:3, findings that were consistent with the enzyme-linked immunosorbent assay data. Because of epitope variations between rotavirus strains, serotyping with several monoclonal antibodies directed at different epitopes may increase the sensitivity of the method. C1 UNIV MARYLAND,SCH MED,CTR VACCINE DEV,BALTIMORE,MD 21201. INT CTR DIARRHOEL DIS RES,DHAKA,BANGLADESH. JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT INT HLTH,BALTIMORE,MD 21205. NIAID,BETHESDA,MD 20892. STANFORD UNIV,MED CTR,SCH MED,DIV GASTROENTEROL,STANFORD,CA 94305. ROYAL CHILDRENS HOSP,PARKVILLE,VIC 3052,AUSTRALIA. UNIV PAVIA,INST INFECT DIS,VIRUS LAB,I-27100 PAVIA,ITALY. RP WARD, RL (reprint author), JAMES N GAMBLE INST MED RES,2141 AUBURN AVE,CINCINNATI,OH 45219, USA. OI Coulson, Barbara/0000-0002-2110-8147 NR 38 TC 49 Z9 49 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAR PY 1991 VL 29 IS 3 BP 449 EP 456 PG 8 WC Microbiology SC Microbiology GA EY463 UT WOS:A1991EY46300008 PM 1709945 ER PT J AU POWERS, DC FRIES, LF MURPHY, BR THUMAR, B CLEMENTS, ML AF POWERS, DC FRIES, LF MURPHY, BR THUMAR, B CLEMENTS, ML TI IN ELDERLY PERSONS LIVE ATTENUATED INFLUENZA-A VIRUS-VACCINES DO NOT OFFER AN ADVANTAGE OVER INACTIVATED VIRUS-VACCINE IN INDUCING SERUM OR SECRETORY ANTIBODIES OR LOCAL IMMUNOLOGICAL MEMORY SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID WILD-TYPE VIRUS; ADULT VOLUNTEERS; RESPONSES; H1N1; POPULATION; INFECTION; PERSISTENCE; RESISTANCE; REDUCTION; MORTALITY AB In a double-blind, randomized trial, 102 healthy elderly subjects were inoculated with one of four preparations: (i) intranasal bivalent live attenuated influenza vaccine containing cold-adapted A/Kawasaki/86 (H1N1) and cold-adapted A/Bethesda/85 (H3N2) viruses; (ii) parenteral trivalent inactivated subvirion vaccine containing A/Taiwan/86 (H1N1), A/Leningrad/86 (H3N2), and B/Ann Arbor/86 antigens; (iii) both vaccines; or (iv) placebo. To determine whether local or systemic immunization augmented mucosal immunologic memory, all volunteers were challenged intranasally 12 weeks later with the inactivated virus vaccine. We used a hemagglutination inhibition assay to measure antibodies in sera and a kinetic enzyme-linked immunosorbent assay to measure immunoglobulin G (IgG) and IgA antibodies in sera and a kinetic enzyme-linked immunosorbent comparison with the live virus vaccine, the inactivated virus vaccine elicited higher and more frequent rises of serum antibodies, while nasal wash antibody responses were similar. The vaccine combination induced serum and local antibodies slightly more often than the inactivated vaccine alone did. Coadministration of live influenza A virus vaccine did not alter the serum antibody response to the influenza B virus component of the inactivated vaccine. The anamnestic nasal antibody response elicited by intranasal inactivated virus challenge did not differ in the live, inactivated, or combined vaccine groups from that observed in the placebo group not previously immunized. These results suggest that in elderly persons cold-adapted influenza A virus vaccines offer little advantage over inactivated virus vaccines in terms of inducing serum or secretory antibody or local immunological memory. Studies are needed to determine whether both vaccines in combination are more efficacious than inactivated vaccine alone in people in this age group. C1 NIA,GERONTOL RES CTR,CLIN IMMUNOL SECT,BALTIMORE,MD 21224. JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV GERIATR MED,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV INFECT DIS,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,CTR IMMUNIZAT RES,BALTIMORE,MD 21218. NIAID,INFECT DIS LAB,BETHESDA,MD 20892. FU PHS HHS [N01-A1-62515] NR 38 TC 59 Z9 63 U1 1 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAR PY 1991 VL 29 IS 3 BP 498 EP 505 PG 8 WC Microbiology SC Microbiology GA EY463 UT WOS:A1991EY46300018 PM 2037667 ER PT J AU ROSA, PA HOGAN, D SCHWAN, TG AF ROSA, PA HOGAN, D SCHWAN, TG TI POLYMERASE CHAIN-REACTION ANALYSES IDENTIFY 2 DISTINCT CLASSES OF BORRELIA-BURGDORFERI SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID LYME-DISEASE SPIROCHETE; EPIZOOTIC BOVINE ABORTION; ADULT IXODES-DAMMINI; INVITRO CULTIVATION; MOLECULAR ANALYSIS; ANTIBODY-RESPONSE; SP-NOV; AGENT; ARTHRITIS; AMPLIFICATION AB We sequenced homologous chromosomal loci from several North American and European isolates of the Lyme disease spirochete Borrelia burgdorferi, as well as from the relapsing fever spirochete Borrelia hermsii. Inter- and intraspecies sequence comparisons permitted the design of B. burgdorferi-specific polymerase chain reaction primers that detected all strains tested (n = 31) from diverse geographical and biological origins. Polymerase chain reaction "typing" with other unique sets of primers subdivided B. burgdorferi isolates into two groups: all North American isolates and a few European isolates made up one group, while the majority of the European and Asian isolates made up the second group. This classification may have a clinical correlate reflected in differences between "typical" Lyme borreliosis in North America and Europe. C1 NIAID,ROCKY MT LABS,VECTORS & PATHOGENS LAB,HAMILTON,MT 59840. RP ROSA, PA (reprint author), NIAID,MICROBIAL STRUCT & FUNCT LAB,HAMILTON,MT 59840, USA. NR 53 TC 131 Z9 132 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAR PY 1991 VL 29 IS 3 BP 524 EP 532 PG 9 WC Microbiology SC Microbiology GA EY463 UT WOS:A1991EY46300022 PM 2037670 ER PT J AU SCHIFFMAN, MH BAUER, HM LORINCZ, AT MANOS, MM BYRNE, JC GLASS, AG CADELL, DM HOWLEY, PM AF SCHIFFMAN, MH BAUER, HM LORINCZ, AT MANOS, MM BYRNE, JC GLASS, AG CADELL, DM HOWLEY, PM TI COMPARISON OF SOUTHERN BLOT HYBRIDIZATION AND POLYMERASE CHAIN-REACTION METHODS FOR THE DETECTION OF HUMAN PAPILLOMAVIRUS DNA SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID INFECTION AB A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by cervicovaginal lavage. The study population included 32 women with condylomatous atypia or cervical intraepithelial neoplasia and 88 control women with no known history of cervical neoplasia. Two laboratories used PCR with different sets of consensus primers for HPV detection. The third laboratory used low-stringency Southern blot hybridization to identify all HPV types, followed by high-stringency Southern and/or dot blot hybridization to confirm specific HPV types. One of the PCR primer sets detected HPV types with a differential efficiency that was not predicted by analysis of DNA sequences or direct testing of HPV-containing plasmids. In contrast, the second PCR primer set was shown to be a much broader consensus system, detecting the same HPV types as Southern blotting, though requiring much less clinical specimen. Over 80% of women with cervical intraepithelial neoplasia or condylomatous atypia were found to be HPV infected both by Southern blotting and by the second PCR primer set. Among the control women, 11% were HPV positive by Southern blotting, while 31% were positive with the second set of primers. Most of the HPV infections found only by PCR were not due to HPV type 6, 11, 16, 18, 31, 33, or 45. These known HPV types were uncommon among normal women in the study population, even as determined by the PCR method. C1 NCI,TUMOR VIRUS BIOL LAB,BETHESDA,MD 20892. CETUS CORP,DEPT INFECT DIS,EMERYVILLE,CA 94608. LIFE TECHNOL INC,CORP RES,GAITHERSBURG,MD 20877. KAISER PERMANENTE ONCOL RES,PORTLAND,OR 97227. WESTAT CORP,ROCKVILLE,MD 20850. RP SCHIFFMAN, MH (reprint author), NCI,ENVIRONM EPIDEMIOL BRANCH,EXECUT PLAZA N,BETHESDA,MD 20892, USA. NR 10 TC 155 Z9 158 U1 0 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAR PY 1991 VL 29 IS 3 BP 573 EP 577 PG 5 WC Microbiology SC Microbiology GA EY463 UT WOS:A1991EY46300031 PM 1645370 ER PT J AU ZIERDT, CH AF ZIERDT, CH TI PATHOGENICITY OF BLASTOCYSTIS-HOMINIS SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Letter ID INFECTION RP ZIERDT, CH (reprint author), NIH,DEPT CLIN PATHOL,SERV MICROBIOL,BETHESDA,MD 20892, USA. NR 12 TC 18 Z9 18 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAR PY 1991 VL 29 IS 3 BP 662 EP 662 PG 1 WC Microbiology SC Microbiology GA EY463 UT WOS:A1991EY46300053 PM 2037690 ER PT J AU ANERUD, A LOE, H BOYSEN, H AF ANERUD, A LOE, H BOYSEN, H TI THE NATURAL-HISTORY AND CLINICAL COURSE OF CALCULUS FORMATION IN MAN SO JOURNAL OF CLINICAL PERIODONTOLOGY LA English DT Article DE NATURAL HISTORY; PERIODONTAL DISEASE; CALCULUS ID PERIODONTAL-DISEASE; AGE AB This paper describes levels and progression of supra- and subgingival calculus undisturbed by active professional intervention or home care between 1970 and 1985 in Sri Lanka, or when removed at regular intervals between 1969 and 1988 in Norway. In the Sri Lankan tea laborers, both supra- and subgingival calculus formation started before age 14 years. At 40 years of age, all participants and almost all teeth and tooth surfaces had calculus. Tea laborers who both smoked tobacco and chewed betel had significantly higher calculus scores than those who only had one of these habits, and those who neither chewed nor smoked had the lowest calculus scores. Teeth with calculus showed a significantly higher rate of loss of attachment than teeth that remained calculus free. For the Norwegians who had enjoyed regular dental care throughout their lives, supragingival calculus did not increase in frequency from adolescence to the forties. Approximately 70% of the interproximal surfaces were calculus free after 40-50 years of age. Subgingival calculus scores, although low, showed some increase with longer times of exposure. On average, each person had 0.4 interproximal surfaces with subgingival calculus as they approached 50 years of age. In this Norwegian population, subgingival calculus had no impact on loss of attachment. C1 NIDR,BETHESDA,MD 20892. NR 28 TC 60 Z9 61 U1 0 U2 5 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0303-6979 J9 J CLIN PERIODONTOL JI J. Clin. Periodontol. PD MAR PY 1991 VL 18 IS 3 BP 160 EP 170 PG 11 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA FC374 UT WOS:A1991FC37400002 PM 2061415 ER PT J AU MCCRAE, RR COSTA, PT AF MCCRAE, RR COSTA, PT TI THE NEO PERSONALITY-INVENTORY - USING THE 5-FACTOR MODEL IN COUNSELING SO JOURNAL OF COUNSELING AND DEVELOPMENT LA English DT Article ID PSYCHOLOGICAL SYMPTOMS; CENTER CLIENTS; ADULT SAMPLE RP MCCRAE, RR (reprint author), NIA,GERONTOL RES CTR,PERSONAL & CONGNIT LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 43 TC 58 Z9 58 U1 1 U2 17 PU AMER COUNSELING ASSOC PI ALEXANDRIA PA 5999 STEVENSON AVE, ALEXANDRIA, VA 22304-3300 SN 0748-9633 J9 J COUNS DEV JI J. Couns. Dev. PD MAR-APR PY 1991 VL 69 IS 4 BP 367 EP 372 PG 6 WC Psychology, Applied SC Psychology GA FA773 UT WOS:A1991FA77300015 ER PT J AU MCCRAE, RR COSTA, PT AF MCCRAE, RR COSTA, PT TI I AM FROM MISSOURI - YOU HAVE GOT TO SHOW ME - A RESPONSE TO MCCRAE AND COSTA - RESPONSE SO JOURNAL OF COUNSELING AND DEVELOPMENT LA English DT Article ID PERSONALITY RP MCCRAE, RR (reprint author), NIA,GERONTOL RES CTR,PERSONAL & COGNIT LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 8 TC 1 Z9 1 U1 0 U2 0 PU AMER COUNSELING ASSOC PI ALEXANDRIA PA 5999 STEVENSON AVE, ALEXANDRIA, VA 22304-3300 SN 0748-9633 J9 J COUNS DEV JI J. Couns. Dev. PD MAR-APR PY 1991 VL 69 IS 4 BP 375 EP 376 PG 2 WC Psychology, Applied SC Psychology GA FA773 UT WOS:A1991FA77300017 ER PT J AU SVENSSON, LA DILL, J SJOLIN, L WLODAWER, A TONER, M BACON, D MOULT, J VEERAPANDIAN, B GILLILAND, GL AF SVENSSON, LA DILL, J SJOLIN, L WLODAWER, A TONER, M BACON, D MOULT, J VEERAPANDIAN, B GILLILAND, GL TI THE CRYSTAL PACKING INTERACTIONS OF 2 DIFFERENT CRYSTAL FORMS OF BOVINE RIBONUCLEASE-A SO JOURNAL OF CRYSTAL GROWTH LA English DT Article ID MACROMOLECULAR STRUCTURES; REFINEMENT; CRYSTALLIZATION; RESOLUTION AB The crystal packing interactions of two crystal forms of bovine Ribonuclease A are compared. One form, grown in 43% t-butanol at pH 5.3, has space group P2(1), one molecule in the asymmetric unit, a = 30.18 angstrom, b = 38.4 angstrom, c = 53.32 angstrom and beta = 105.85-degrees. The other crystal form is grown in 3M CsCl and acetate buffer at pH 5.1, has space group P2(1)2(1)2(1), two molecules in the asymmetric unit, a = 53.14 angstrom, b = 64.61 angstrom and c = 73.64 angstrom. An analysis of the crystal packing interactions, including solvent bridging, indicates that the high-alcohol crystal form of Ribonuclease A has a balance of hydrogen bonding, electrostatic and Van der Waals interactions. In contrast, the high-salt crystal packing forces are dominated by Van der Waals interactions, but at the dimer interface of the molecules in this crystal form the interactions have a large electrostatic component. The electrostatic interactions of the dimer may be due to a rearrangement in the charge-charge interactions at the active site, a direct result of the presence of the inhibitor, deoxythymidine, covalently bound to His 12. C1 UNIV MARYLAND,MARYLAND BIOTECHNOL INST,CTR ADV RES BIOTECHNOL,SHADY GROVE,9600 GUDELSKY DR,ROCKVILLE,MD 20850. NATL INST STAND & TECHNOL,CTR CHEM TECHNOL,GAITHERSBURG,MD 20899. CHALMERS UNIV TECHNOL,DEPT INORGAN CHEM,S-41296 GOTHENBURG,SWEDEN. NCI,FREDERICK CANC RES FACIL,CRYSTALLOG LAB,BRI,BASIC RES PROGRAM,FREDERICK,MD 21701. RI Sjolin, Lennart/B-2619-2010 NR 17 TC 14 Z9 14 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-0248 J9 J CRYST GROWTH JI J. Cryst. Growth PD MAR PY 1991 VL 110 IS 1-2 BP 119 EP 130 PG 12 WC Crystallography; Materials Science, Multidisciplinary; Physics, Applied SC Crystallography; Materials Science; Physics GA FF694 UT WOS:A1991FF69400016 ER PT J AU SJOLIN, L WLODAWER, A BERGQVIST, G HOLM, P LOTH, K MALMSTROM, H ZAAR, J SVENSSON, LA GILLILAND, GL AF SJOLIN, L WLODAWER, A BERGQVIST, G HOLM, P LOTH, K MALMSTROM, H ZAAR, J SVENSSON, LA GILLILAND, GL TI PROTEIN CRYSTAL-GROWTH OF RIBONUCLEASE-A AND PANCREATIC TRYPSIN-INHIBITOR ABOARD THE MASER-3 ROCKET SO JOURNAL OF CRYSTAL GROWTH LA English DT Article ID LEAST-SQUARES REFINEMENT; X-RAY REFINEMENT; JOINT NEUTRON; RESOLUTION; FORM AB The growth of bovine Ribonuclease A (RNase A) and bovine Pancreatic Trypsin Inhibitor crystals has been studied simultaneously aboard MASER 3, a sounding rocket, and in a ground reference unit. For crystallization to take place within the 7 min and 15 s of microgravity during the flight, both macro- and micro-seed techniques of supersaturated protein solutions were employed. It was found that the convection-free environment of the MASER 3 protein crystal growth apparatus produced more and larger crystals than the ground reference unit. The RNase A crystals grown in microgravity diffracted X-rays to 1.06 angstrom resolution, approximately 0.2 angstrom higher resolution than previously observed in terrestrially grown RNase A crystals. The refined structure based on these data contains new features not seen in the lower resolution structures. C1 UNIV MARYLAND,MARYLAND BIOTECHNOL INST,CTR ADV RES BIOTECHNOL,SHADY GROVE,9600 GUDELSKY DR,ROCKVILLE,MD 20878. CHALMERS UNIV TECHNOL,DEPT INORGAN CHEM,S-41296 GOTHENBURG,SWEDEN. NCI,FREDERICK CANC RES FACIL,CRYSTALLOG LAB,BRI,BASIC RES PROGRAM,FREDERICK,MD 21701. SWEDISH SPACE AGCY,S-17154 SOLNA,SWEDEN. NATL INST STAND & TECHNOL,CTR CHEM TECHNOL,GAITHERSBURG,MD 20899. RI Sjolin, Lennart/B-2619-2010 NR 13 TC 8 Z9 9 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-0248 J9 J CRYST GROWTH JI J. Cryst. Growth PD MAR PY 1991 VL 110 IS 1-2 BP 322 EP 332 DI 10.1016/0022-0248(91)90902-H PG 11 WC Crystallography; Materials Science, Multidisciplinary; Physics, Applied SC Crystallography; Materials Science; Physics GA FF694 UT WOS:A1991FF69400044 ER PT J AU KRICHEVSKY, MI AF KRICHEVSKY, MI TI DRAFT RESULTS OF A WORKSHOP TO DEVELOP GUIDELINES FOR STUDIES INVOLVING MICROBIAL INCIDENCE OR POPULATIONS IN THE ORAL CAVITY SO JOURNAL OF DENTAL RESEARCH LA English DT Editorial Material AB The following five outlines are the results to date of the Workshop held in Rockville, Maryland, in January, 1990. The topics considered in these outlines are: (1) validation of immunological and/or nucleic acid identification probes, (2) cross-calibration of methods and/or laboratories for multi-laboratory cooperative studies, (3) choosing methods for identifying or describing microbial populations appropriate to the scientific question asked, (4) microbial ecology methods (e.g., population dynamics) for the oral cavity studies, and (5) epidemiological methods (e.g., incidence, risk factor analysis) for oral microbial studies. Each topic was considered by two independent groups of participants and later rationalized into one. These outlines are meant to be working outlines for evolution of a set of guidelines to advise on designing studies with microbial incidence and/or population components. We are publishing this preliminary version to elicit comment and criticism from people who did not attend the Workshop. (Attendance at the Workshop was necessarily limited by both space and funds.) Some of the topic outlines have been condensed to save Journal space. The full document is available on request. The next stage will be an open forum to gather and discuss further amplification of the "Guidelines", planned for April 17, 1991, Acapulco, Mexico, in conjunction with the IADR/AADR Meeting. Written comments and requests for further information should be sent to the Workshop organizer (MIK) at the above address. Within each topic, the editing was done in two phases. The basic outlines were prepared by the "break-out" groups during the Workshop. The second stage was to annotate the outlines with the organizer's editorial comments and suggestions. The annotations are set off by brackets. In the main, these consist of requests for amplification and clarification. In a few cases, there may well need to be a fresh look at some of the concepts presented. Three come to mind immediately: (1) the use of diversity measures, (2) "gold standards", and (3) the use of community structure analysis as presented herein. Others may occur to the reader. You are invited to make any and all comments on the material presented in these outlines. Please let your comments range from syntax and format through detailed and broad observations on content and omission. Especially welcome will be your comments on the annotated remarks and any annotations you feel should be added. For example, should reprints be attached to the guidelines, or should the contents of relevant papers be synthesized (or some combination thereof)? RP KRICHEVSKY, MI (reprint author), NIDR,EPIDEMIOL & ORAL DIS PREVENT PROGRAM,MICROBIAL SYSTEMAT SECT,BETHESDA,MD 20892, USA. NR 2 TC 4 Z9 4 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PD MAR PY 1991 VL 70 IS 3 BP 226 EP 232 DI 10.1177/00220345910700031401 PG 7 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA FD585 UT WOS:A1991FD58500014 PM 1671870 ER PT J AU ALDAMLUJI, S AF ALDAMLUJI, S TI MEASURING THE ACTIVITY OF BRAIN ADRENERGIC-RECEPTORS IN MAN SO JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION LA English DT Review DE ADRENERGIC RECEPTORS; CATECHOLAMINES; ADRENOCORTICOTROPIC HORMONE; CORTICOTROPIN RELEASING FACTOR; VASOPRESSIN; CORTISOL ID CORTICOTROPIN-RELEASING-FACTOR; HUMAN CEREBROSPINAL-FLUID; RAT PITUITARY-GLAND; PARAVENTRICULAR NUCLEUS; ACTH-SECRETION; ADRENOCORTICOTROPIN RELEASE; BETA-ENDORPHIN; BETA-2-ADRENERGIC RECEPTORS; STIMULATES SECRETION; TYROSINE-HYDROXYLASE RP ALDAMLUJI, S (reprint author), NIH,ENDOCRINOL & REPROD RES BRANCH,BLDG 10,ROOM B1L 400,BETHESDA,MD 20892, USA. NR 90 TC 9 Z9 9 U1 1 U2 1 PU EDITRICE KURTIS S R L PI MILANO PA VIA LUIGI ZOJA, 30-20153 MILANO, ITALY SN 0391-4097 J9 J ENDOCRINOL INVEST JI J. Endocrinol. Invest. PD MAR PY 1991 VL 14 IS 3 BP 245 EP 254 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FL247 UT WOS:A1991FL24700014 PM 1649215 ER PT J AU NOGUCHI, S OHBA, Y OKA, T AF NOGUCHI, S OHBA, Y OKA, T TI INFLUENCE OF EPIDERMAL GROWTH-FACTOR ON LIVER-REGENERATION AFTER PARTIAL-HEPATECTOMY IN MICE SO JOURNAL OF ENDOCRINOLOGY LA English DT Article ID RAT-LIVER; SUBMANDIBULAR-GLAND AB The role of epidermal growth factor (EGF) in liver regeneration was studied in mice after partial hepatectomy. Two weeks before partial hepatectomy, mice were sham-operated (control) or sialoadenectomized (removal of submandibular glands) to reduce plasma EGF levels. Sialoadenectomized mice showed low plasma EGF levels (29.7 +/- 6.6 pmol/l; mean +/- S.E.M.) compared with controls (66.0 +/- 8.3 pmol/l). After partial hepatectomy, sialoadenectomized mice were treated with or without a daily s.c. injection of 5-mu-g EGF and the rate of DNA synthesis in the regenerating liver was monitored by [I-125]iododeoxyuridine uptake. Control mice showed a sharp peak of DNA synthesis at 48 h after partial hepatectomy while sialoadenectomized mice showed a delayed and broad peak at 84 h. Treatment of sialoadenectomized mice with EGF (5-mu-g/mouse per day) completely restored the pattern of DNA synthesis so that a sharp peak appeared at 48 h. The total liver DNA content of the control mice (79.1 +/- 2.5% of the preoperative level; mean +/- S.E.M.) was significantly (P < 0.01) higher than that of the sialoadenectomized mice (65.2 +/- 3.0%) 3 days after partial hepatectomy, but this difference disappeared on day 7 when liver regeneration was almost completed in both groups. Treatment of sialoadenectomized mice with EGF increased total liver DNA content (78.2 +/- 2.9%) to that of control mice on day 3 after partial hepatectomy. In addition, normal mice showed a rapid increase in plasma EGF levels at 1-8 h after partial hepatectomy, whereas sialoadenectomized mice showed low plasma EGF levels throughout the course of the experiment. These results suggest that EGF derived from the submandibular glands plays a role in promoting the early stage of liver regeneration. C1 NIDDKD,MOLEC & CELLULAR BIOL LAB,BLDG 8,ROOM 319,BETHESDA,MD 20892. NR 26 TC 93 Z9 94 U1 0 U2 0 PU J ENDOCRINOLOGY LTD PI BRISTOL PA 17/18 THE COURTYARD, WOODLANDS, ALMONDSBURY, BRISTOL, ENGLAND BS12 4NQ SN 0022-0795 J9 J ENDOCRINOL JI J. Endocrinol. PD MAR PY 1991 VL 128 IS 3 BP 425 EP 431 DI 10.1677/joe.0.1280425 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FB080 UT WOS:A1991FB08000014 PM 2013747 ER PT J AU POLI, G KINTER, AL JUSTEMENT, JS BRESSLER, P KEHRL, JH FAUCI, AS AF POLI, G KINTER, AL JUSTEMENT, JS BRESSLER, P KEHRL, JH FAUCI, AS TI TRANSFORMING GROWTH-FACTOR-BETA SUPPRESSES HUMAN-IMMUNODEFICIENCY-VIRUS EXPRESSION AND REPLICATION IN INFECTED-CELLS OF THE MONOCYTE MACROPHAGE LINEAGE SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID TUMOR NECROSIS FACTOR; IMMUNE-DEFICIENCY SYNDROME; INTRAVENOUS DRUG-ABUSERS; NATURAL-KILLER CELLS; NF-KAPPA-B; FACTOR-BETA; FACTOR-ALPHA; LYMPHADENOPATHY SYNDROME; CYTOKINE PRODUCTION; HUMAN-LYMPHOCYTES AB The pleiotropic immunoregulatory cytokine transforming growth factor-beta (TGF-beta) potently suppresses production of the human immunodeficiency virus (HIV), the causative agent of the acquired immunodeficiency syndrome, in the chronically infected promonocytic cell line U1. TGF-beta significantly (50-90%) inhibited HIV reverse transcriptase production and synthesis of viral proteins in U1 cells stimulated with phorbol myristate acetate (PMA) or interleukin 6 (IL-6). Furthermore, TGF-beta suppressed PMA induction of HIV transcription in U1 cells. In contrast, TGF-beta did not significantly affect the expression of HIV induced by tumor necrosis factor-alpha (TNF-alpha). These suppressive effects were not mediated via the induction of interferon-alpha (IFN-alpha). TGF-beta also suppressed HIV replication in primary monocyte-derived macrophages infected in vitro, both in the absence of exogenous cytokines and in IL-6-stimulated cultures. In contrast, no significant effects of TGF-beta were observed in either a chronically infected T cell line (ACH-2) or in primary T cell blasts infected in vitro. Therefore, TGF-beta may play a potentially important role as a negative regulator of HIV expression in infected monocytes or tissue macrophages in infected individuals. RP POLI, G (reprint author), NIAID,IMMUNOREGUL LAB,BLDG 10,ROOM 11B-13,BETHESDA,MD 20892, USA. NR 64 TC 152 Z9 152 U1 0 U2 2 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD MAR 1 PY 1991 VL 173 IS 3 BP 589 EP 597 DI 10.1084/jem.173.3.589 PG 9 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA EZ663 UT WOS:A1991EZ66300008 PM 1705278 ER PT J AU BARTH, RJ MULE, JJ SPIESS, PJ ROSENBERG, SA AF BARTH, RJ MULE, JJ SPIESS, PJ ROSENBERG, SA TI INTERFERON-GAMMA AND TUMOR-NECROSIS-FACTOR HAVE A ROLE IN TUMOR REGRESSIONS MEDIATED BY MURINE CD8+ TUMOR-INFILTRATING LYMPHOCYTES SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID DELAYED-TYPE HYPERSENSITIVITY; MACROPHAGE-ACTIVATING FACTOR; CYTOTOXIC T-CELLS; ADOPTIVE IMMUNOTHERAPY; GAMMA-INTERFERON; IFN-GAMMA; INVITRO INDUCTION; INDUCED SARCOMA; INVIVO; IMMUNITY AB We have investigated the mechanisms whereby adoptively transferred murine CD8+ lymphocytes mediate tumor regressions. Noncytolytic, CD8+ tumor-infiltrating lymphocytes (TIL) eradicated established lung tumors in irradiated mice. Many cytolytic and noncytolytic CD8+ TIL cultures specifically secreted interferon-gamma (IFN-gamma) and tumor necrosis factor when stimulated with tumor cells in vitro. The effectiveness of TIL when adoptively transferred to mice bearing micrometastases correlated better with their ability to specifically secrete lymphokines than with their cytotoxicity in vitro. In 14 of 15 tests, therapeutically effective TIL specifically secreted IFN-gamma in vitro, whereas only 1 of 11 ineffective TIL specifically secreted IFN-gamma. In contrast, only 8 of 15 therapeutically effective TIL were cytolytic. Antibodies to TNF inhibited the effectiveness of two adoptively transferred TIL cultures. In five experiments, antibodies to IFN-gamma abrogated the ability of four different CD8+ TIL cultures to mediate tumor regressions, indicating that secretion of IFN-gamma is an essential part of the mechanism of action of TIL. C1 NCI,SURG BRANCH,BLDG 10,ROOM 2B46,BETHESDA,MD 20892. NR 48 TC 396 Z9 400 U1 0 U2 3 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD MAR 1 PY 1991 VL 173 IS 3 BP 647 EP 658 DI 10.1084/jem.173.3.647 PG 12 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA EZ663 UT WOS:A1991EZ66300014 PM 1900079 ER PT J AU JOHNSTON, LA DONOGHUE, AM OBRIEN, SJ WILDT, DE AF JOHNSTON, LA DONOGHUE, AM OBRIEN, SJ WILDT, DE TI CULTURE-MEDIUM AND PROTEIN SUPPLEMENTATION INFLUENCE INVITRO FERTILIZATION AND EMBRYO DEVELOPMENT IN THE DOMESTIC CAT SO JOURNAL OF EXPERIMENTAL ZOOLOGY LA English DT Article ID CELL MOUSE EMBRYOS; BOVINE SERUM-ALBUMIN; BLASTOCYST STAGE; EPIDIDYMAL SPERMATOZOA; SUPPORTS DEVELOPMENT; FOLLICULAR OOCYTES; HAMSTER EMBRYOS; RABBIT EMBRYOS; AMINO-ACIDS; CO-CULTURE AB The influence of culture medium and protein supplements on in vitro fertilization (IVF) and morula-to-blastocyst development in culture was examined in the domestic cat. In Study I, follicular oocytes were fertilized and cultured in 1) modified Krebs Ringer bicarbonate (mKRB); 2) modified Tyrode's solution (TALP) without phosphate or glucose; or 3) Ham's F10. All media contained bovine serum albumin (BSA). Fertilization rates were similar (P > .05) among mKRB (75.0%), TALP (70.6%), and Ham's F10 (80.0%) treatments. Compared to TALP (77.8%), more (P < .05) embryos in Ham's F10 (95.0%) developed to the morula stage; development of mKRB embryos (88.9%) was intermediate and not different (P > .05). Study II evaluated the effects of protein availability and type on IVF and embryo growth. Ham's F10 was supplemented with polyvinylal-cohol (PVA, 2 mg/ml), BSA (4 mg/ml), fetal calf serum (FCS, 5%), or estrous cat serum (ECS, 5%) and used to fertilize and culture embryos. Fertilization was enhanced (P < .05) using either FCS (84.0%) or ECS (85.2%) compared to PVA (67.3%). Oocytes exposed to BSA fertilized at a rate (76.1%) similar (P > .05) to other treatments. Embryos exposed to all four treatment groups (PVA, 82.8%; BSA, 82.8%; FCS, 92.9%; or ECS, 97.8%) were equally capable (P > .05) of becoming morulae. However, more FCS- or ECS-supplemented morulae continued to the early blastocyst stage (30.8%, 22.2%, respectively, P < .05) than PVA- (10.3%) or BSA- (13.8%) exposed morulae. In the domestic cat IVF system, the type of medium and protein supplement used appears to have a greater impact on embryo development in vitro than on fertilization. There also is a morula-to-blastocyst developmental block for cat embryos resulting from IVF which is partially circumvented by adding serum to the culture medium. Ham's F10 containing FCS or ECS produces the highest incidence of fertilization (approximately 85%), and approximately 2-fold more of these embryos develop in culture to blastocysts compared to embryos supplemented with PVA or BSA. C1 SMITHSONIAN INST,NATL ZOOL PK,WASHINGTON,DC 20008. NCI,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21701. NR 54 TC 54 Z9 55 U1 1 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0022-104X J9 J EXP ZOOL JI J. Exp. Zool. PD MAR PY 1991 VL 257 IS 3 BP 350 EP 359 DI 10.1002/jez.1402570308 PG 10 WC Zoology SC Zoology GA FC254 UT WOS:A1991FC25400007 PM 2005424 ER PT J AU HEITMAN, CK INNES, CL JETTEN, AM BOONE, LR AF HEITMAN, CK INNES, CL JETTEN, AM BOONE, LR TI ANALYSIS OF FV-1 RESTRICTION IN 2 MURINE EMBRYONAL CARCINOMA CELL-LINES AND A SERIES OF DIFFERENTIATED DERIVATIVES SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID LOCUS AFFECTING RESISTANCE; HOST-RANGE RESTRICTIONS; FRIEND-LEUKEMIA VIRUS; RETINOIC ACID; MOUSE FIBROBLASTS; GENE-EXPRESSION; PROVIRAL DNA; STEM-CELLS; INFECTION; RETROVIRUS AB We have used antibiotic-resistant retrovirus vectors rescued by Fv-1-sensitive murine leukaemia viruses (MuLV) to examine the Fv-1 phenotype of two undifferentiated embryonal carcinoma (EC) cell lines derived from teratocarcinomas of mouse strain 129. In addition, a set of EC cell-derived differentiated cell lines was analysed. Restriction of both B-tropic and endogenous N-tropic virus is characteristic of the N(r)-type restriction reported in mouse strain 129. However, results indicate that Fv-1 restriction is not expressed in the PCC4.aza1R EC cell line. In contrast, the F9 EC cell line showed a strong restriction of the B-tropic pseudotyped vector but failed to restrict endogenous N-tropic pseudotypes. The Fv-1 gene thus seems to be differentially expressed in two EC cell lines derived from the same mouse strain. Furthermore, the selective restriction of B-tropic but not endogenous N-tropic MuLV in F9 cells suggests that these activities function independently of each other. Analysis of PCC4.aza1R-derived differentiated cell lines revealed that three fibroblast cell lines derived by retinoic acid-induced differentiation were also phenotypically silent for Fv-1. However, a pre-adipocyte line established following simultaneous exposure to retinoic acid and 5-azacytidine showed strong restriction of both B-tropic and endogenous N-tropic MuLV. Although additional data suggest that there is no correlation between the differentiated pre-adipocyte phenotype and Fv-1 expression, our results nonetheless show that N(r) restriction can be observed in some derivatives of PCC4.aza1R cells, presumably by activating expression of the Fv-1 gene. C1 NIEHS,PULM PATHOBIOL LAB,RES TRIANGLE PK,NC 27709. NIEHS,CELLULAR & GENET TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. NR 48 TC 2 Z9 2 U1 0 U2 0 PU SOC GENERAL MICROBIOLOGY PI READING PA HARVEST HOUSE 62 LONDON ROAD, READING, BERKS, ENGLAND RG1 5AS SN 0022-1317 J9 J GEN VIROL JI J. Gen. Virol. PD MAR PY 1991 VL 72 BP 609 EP 616 DI 10.1099/0022-1317-72-3-609 PN 3 PG 8 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA FB091 UT WOS:A1991FB09100019 PM 1848595 ER PT J AU GROSSMAN, E HOFFMAN, A TAMRAT, M ARMANDO, I KEISER, HR GOLDSTEIN, DS AF GROSSMAN, E HOFFMAN, A TAMRAT, M ARMANDO, I KEISER, HR GOLDSTEIN, DS TI ENDOGENOUS DOPA AND DOPAMINE RESPONSES TO DIETARY SALT LOADING IN SALT-SENSITIVE RATS SO JOURNAL OF HYPERTENSION LA English DT Article DE DOPAMINE; DOPA; SODIUM; SALT; NATRIURESIS ID URINARY DOPAMINE; EXCRETION; SODIUM; PLASMA; DIHYDROXYPHENYLALANINE; HYPERTENSION; KIDNEY; TUBULE AB We measured daily urinary excretion rates of dopamine and dopa during dietary salt loading and natriuretic responses to exogenous dopamine in Dahl salt-sensitive (DS), Dahl salt-resistant (DR) and Sprague-Dawley rats. Excretion rates of dopa increased approximately sixfold during salt loading in all rat strains. Maximal urinary dopa responses were attained within 1 day of salt loading. Daily excretion rates of dopamine also increased about five- to sixfold in DS and DR rats and about twofold in Sprague-Dawley rats, with maximal dopamine responses attained by day 5. Dopamine infusion (3-mu-g/kg per min) increased urinary sodium excretion by 406 +/- 132 % (mean +/- s.e.m.) in Sprague-Dawley rats but only 267 +/- 131% and 147 +/- 80% in DS and DR rats (P < 0.05 for Sprague-Dawley versus Dahl rats). The results demonstrate that salt loading markedly and rapidly increases dopa excretion in rats. Considering values for dopamine excretion in other rat strains, the results suggest that Dahl rats have increased formation of dopamine for a given amount of dopa delivery to the kidney and that this abnormality is unrelated to salt-sensitive hypertension in DS rats. The results also provide in vivo support for the view that the responsiveness of renal dopamine receptors mediating natriuresis is related to production of endogenous dopamine in the kidney. C1 NHLBI,HYPERTENS ENDOCRINE BRANCH,BETHESDA,MD 20892. NR 16 TC 31 Z9 33 U1 0 U2 0 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0263-6352 J9 J HYPERTENS JI J. Hypertens. PD MAR PY 1991 VL 9 IS 3 BP 259 EP 263 DI 10.1097/00004872-199103000-00010 PG 5 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA FE351 UT WOS:A1991FE35100010 PM 1851789 ER PT J AU MURRAY, GJ HOWARD, KD RICHARDS, SM BARTON, NW BRADY, RO AF MURRAY, GJ HOWARD, KD RICHARDS, SM BARTON, NW BRADY, RO TI GAUCHERS-DISEASE - LACK OF ANTIBODY-RESPONSE IN 12 PATIENTS FOLLOWING REPEATED INTRAVENOUS INFUSIONS OF MANNOSE TERMINAL GLUCOCEREBROSIDASE SO JOURNAL OF IMMUNOLOGICAL METHODS LA English DT Article DE GAUCHERS DISEASE; ELISA; GLUCOCEREBROSIDASE; INTRAVENOUS INFUSION; ENZYME AMPLIFICATION; (CLINICAL) ID ENZYME AMPLIFICATION; CELLS; GLYCOPROTEINS; IMMUNOASSAYS AB An amplified ELISA has been employed for monitoring the safety of repeated intravenous infusions of modified human placental glucocerebrosidase. The enzyme infusions consisted of biweekly injections of macrophage targeted glucocerebrosidase over a 6 month duration. Serum samples collected throughout the study were assayed by use of an ELISA using alkaline phosphatase coupled to alcohol dehydrogenase for amplification. Using this protocol, 0.2-5 ng affinity purified immunoglobulin specific for glucocerebrosidase can be detected. Occasional false positives necessitate multiple repeat assays over time to accurately assess immunogenic response. Blinded ELISAs were performed on sera from both infused patients with Gaucher's disease and uninfused control patients and compared with apparent immunoglobulin concentration in 54 normal control sera. Although several samples showed apparently elevated immunoglobulin levels, repeat analyses failed to demonstrate high levels reproducibly. Furthermore, these sera were unable to neutralize enzyme or to precipitate radiolabelled enzyme, confirming the absence of antibody. Problems with high sensitivity ELISA formats are discussed. C1 GENZYME CORP,FRAMINGHAM,MA. RP MURRAY, GJ (reprint author), NIH,DEV & METAB NEUROL BRANCH,BLDG 10,ROOM 3D-04,BETHESDA,MD 20892, USA. NR 25 TC 10 Z9 10 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1759 J9 J IMMUNOL METHODS JI J. Immunol. Methods PD MAR 1 PY 1991 VL 137 IS 1 BP 113 EP 120 DI 10.1016/0022-1759(91)90400-A PG 8 WC Biochemical Research Methods; Immunology SC Biochemistry & Molecular Biology; Immunology GA FB240 UT WOS:A1991FB24000014 PM 2010615 ER PT J AU KIRSHENBAUM, AS KESSLER, SW GOFF, JP METCALFE, DD AF KIRSHENBAUM, AS KESSLER, SW GOFF, JP METCALFE, DD TI DEMONSTRATION OF THE ORIGIN OF HUMAN MAST-CELLS FROM CD34+ BONE-MARROW PROGENITOR CELLS SO JOURNAL OF IMMUNOLOGY LA English DT Article ID BASOPHIL-LIKE CELLS; COMMITTED PROGENITOR; HEMATOPOIETIC-CELLS; DIFFERENTIATION; FIBROBLASTS; INVITRO; PROLIFERATION; INTERLEUKIN-3; MICE; GRANULOCYTES AB It has been established that murine mast cells are derived from a pluripotent bone marrow stem cell. In humans, the corresponding pluripotent cell is included in the CD34+ bone marrow population. To determine whether human mast cells arise from CD34+ human progenitor cells, enriched CD34+ cells were cultured over agarose surfaces (interphase cultures) or cocultured with mouse 3T3 fibroblasts in the presence of recombinant human (rh) IL-3. The presence of both mast cells and basophils was determined using a variety of histochemical and immunohistologic techniques, including immunogold labeling for IgE receptors and mast cell tryptase. Mast cells and basophils continued to appear in cultures when T cell, B cell, macrophage, and eosinophil committed progenitor cells were removed, but were not seen in cultures from which CD34+ cells were removed. CD34+ cells layered over agarose in the presence of rhIL-3 were shown to give rise to cultures that contained mast cells (1 to 5%) and basophils (25 to 40%). Cultures supplemented with rhIL-4 showed no additional increase in mast cells or basophils. CD34+ cells cocultured with 3T3 fibroblasts in the presence of rhIL-3 gave rise to mast cells within the fibroblast monolayer, which by 6 wk comprised up to 46% of the monolayer. CD34- cells on 3T3 fibroblasts gave rise to few mast cells (2% of the monolayer). Mast cell granules from interphase cultures contained homogeneous electron-dense material. In contrast, mast cells within 3T3 monolayers at 6 wk contained a variety of granule morphologies, including scroll, mixed, reticular, dense core, or homogeneous patterns. We conclude that both human mast cells and basophils arise from CD34+ human progenitor cells. C1 GEORGETOWN UNIV,MED CTR,DIV MOLEC VIROL & IMMUNOL,ROCKVILLE,MD 20852. HOMEWOOD HOSP,DEPT MED,DIV ALLERGY,BALTIMORE,MD 21211. USN,MED RES INST,DEPT IMMUNOL & TRANSPLANTAT,BETHESDA,MD 20889. RP KIRSHENBAUM, AS (reprint author), NIAID,LCI,MAST CELL PHYSIOL SECT,BLDG 10,ROOM 11C212,BETHESDA,MD 20892, USA. NR 31 TC 279 Z9 283 U1 0 U2 4 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 1 PY 1991 VL 146 IS 5 BP 1410 EP 1415 PG 6 WC Immunology SC Immunology GA EY684 UT WOS:A1991EY68400004 PM 1704394 ER PT J AU KOSUGI, A SHEARER, GM AF KOSUGI, A SHEARER, GM TI EFFECT OF CYCLOSPORINE-A ON LYMPHOPOIESIS .3. AUGMENTATION OF THE GENERATION OF NATURAL-KILLER-CELLS IN BONE-MARROW TRANSPLANTED MICE TREATED WITH CYCLOSPORINE-A SO JOURNAL OF IMMUNOLOGY LA English DT Article ID ASIALO GM1 ANTIBODY; CYTO-TOXIC ACTIVITY; NK CELLS; T-CELLS; INTERLEUKIN-2 IL-2; IMMUNE-SYSTEM; HOST DISEASE; PRECURSORS; DISTINCT; RECEPTOR AB The effects of cyclosporin A (CsA) on the generation of NK cells were studied using syngeneic bone marrow transplanted mice subsequently treated with CsA (BMT/CsA mice). In contrast to a severe reduction in T cells that was reported previously, these mice exhibited a marked enhancement of splenic NK activity. The enhanced NK activity was mediated by NK1.1+, Thy-1- cells as assessed by antibody plus complement treatment, and was concomitant with an absolute increase in the numbers of NK1.1+ cells as assessed by flow cytometry. Because the depletion of host-derived, mature NK cells by injection of anti-asialo GM1 antibody before bone marrow reconstitution did not affect the enhancement of NK activity, CsA appeared to augment the generation of NK cells from bone marrow precursors. To investigate a possible relationship between the enhancement of NK activity and the maturational arrest of T cells in the thymus induced by CsA, mice were thymectomized, followed by irradiation, bone marrow reconstitution, and CsA treatment. These mice exhibited as strong enhancement of splenic NK activity as BMT/CsA mice, suggesting that the CsA-induced effect on NK cells is distinct from its effect on T cell development in the thymus. Taken together, these results are the first demonstration of the positive effect of CsA on NK cell generation and may be of importance in clinical bone marrow transplantation. RP KOSUGI, A (reprint author), NCI,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4B-17,BETHESDA,MD 20892, USA. NR 41 TC 25 Z9 25 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 1 PY 1991 VL 146 IS 5 BP 1416 EP 1421 PG 6 WC Immunology SC Immunology GA EY684 UT WOS:A1991EY68400005 PM 1993836 ER PT J AU MURAKAMI, S MIYAKE, K ABE, R KINCADE, PW HODES, RJ AF MURAKAMI, S MIYAKE, K ABE, R KINCADE, PW HODES, RJ TI CHARACTERIZATION OF AUTOANTIBODY-SECRETING B-CELLS IN MICE UNDERGOING STIMULATORY (CHRONIC) GRAFT-VERSUS-HOST REACTIONS - IDENTIFICATION OF A CD44HI POPULATION THAT BINDS SPECIFICALLY TO HYALURONATE SO JOURNAL OF IMMUNOLOGY LA English DT Article ID MAJOR HISTOCOMPATIBILITY COMPLEX; MONOCLONAL-ANTIBODIES; CROSS-LINKING; INCOMPATIBLE STRUCTURES; INCREASED EXPRESSION; ANTIGEN EXPRESSION; LYMPHOCYTES-T; IA ANTIGENS; IMMUNOGLOBULIN; ACTIVATION AB This study was undertaken to identify and isolate the pathophysiologically important B cell subpopulation which is activated to Ig secretion and autoantibody production in stimulatory (chronic) graft-vs-host (GVH) reactions. We recently demonstrated that IL-5 stimulation in vitro induces the appearance of a distinct CD44hi Ia(low) CD45R(low) B cell subpopulation that has aquired the ability to bind to hyaluronate (HA), one of the ligands for CD44, and that this B cell subpopulation is enriched in both proliferative and Ig-secretory responses. In the present report, CD44 expression was examined in B cells which were activated in the course of stimulatory GVH reactions. Compared with normal mice, B cells from mice undergoing stimulatory GVH reactions contained a novel CD44hi B cell subpopulation which exhibited enhanced binding to HA. The CD44hi HA-adherent B cell subpopulation from GVH mice spontaneously secreted large amounts of Ig, particularly IgG, including autoantibody specific for ssDNA. These findings demonstrate that CD44 expression distinguishes those B cells that are activated in vivo in the course of GVH to proliferate and differentiate into Ig-secreting cells. These CD44hi, HA-adherent, cells may play a prominent role in the hypergammaglobulinemia and immune complex glomerulonephritis that occur during chronic GVH reactions. C1 NCI,EXPTL IMMUNOL BRANCH,BLDG 10,RM 4B17,BETHESDA,MD 20892. OKLAHOMA MED RES FDN,OKLAHOMA CITY,OK 73104. FU NIAID NIH HHS [AI-20069, AI-19884] NR 35 TC 51 Z9 51 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 1 PY 1991 VL 146 IS 5 BP 1422 EP 1427 PG 6 WC Immunology SC Immunology GA EY684 UT WOS:A1991EY68400006 PM 1993837 ER PT J AU HAMMOND, SA OBAH, E STANHOPE, P MONELL, CR STRAND, M ROBBINS, FM BIAS, WB KARR, RW KOENIG, S SILICIANO, RF AF HAMMOND, SA OBAH, E STANHOPE, P MONELL, CR STRAND, M ROBBINS, FM BIAS, WB KARR, RW KOENIG, S SILICIANO, RF TI CHARACTERIZATION OF A CONSERVED T-CELL EPITOPE IN HIV-1 GP41 RECOGNIZED BY VACCINE-INDUCED HUMAN CYTOLYTIC T-CELLS SO JOURNAL OF IMMUNOLOGY LA English DT Article ID TOXIC LYMPHOCYTES-T; BIND IMMUNOGENIC PEPTIDES; COMPLEX MHC RESTRICTION; HLA-DP POLYMORPHISM; B SURFACE-ANTIGEN; SYNTHETIC PEPTIDES; INFLUENZA-VIRUS; MONOCLONAL-ANTIBODIES; HTLV-III; GENOMIC DIVERSITY AB A human CTL epitope located in a region of the HIV-1 envelope protein gp41 that is highly conserved among various HIV-1 strains was identified. This epitope was recognized by CD4+ CTL clones that were induced in seronegative humans by immunization with recombinant gp160. Fusion proteins carrying portions of the HIV-1 env gene and synthetic peptides were used to localize this epitope to amino acids 584-595 of the HIV-1 BRU env sequence. Only two positions within this epitope showed variation among North American HIV-1 isolates, and the substitutions were conservative in nature. The Lys to Arg substitution at position 593 abolished recognition, probably by interfering with the peptide-MHC interactions. This epitope was recognized in association with at least one subtype of the widely distributed human class II MHC specificity DPw4, namely DPw4.2. The relatively high frequency of this allele (27.2% among Caucasians) makes it likely that a larger fraction of the population would generate a response directed at this epitope than would be the case for epitopes recognized in the context of gene products of most other class II and class I loci. Interestingly, the closely related DP beta-chain allele types 4.1 and 2.1, which differ from 4.2 by 3 and 1 amino acids, respectively, were unable to present this gp41 peptide to DPw4.2-restricted clones. Comparison of the structure of this epitope with that of other peptides recognized in the context of DPw4.2 led to the identification of a consensus sequence for DPw4.2 binding peptides. Because the gp41 CTL epitope 584-595 identified here is highly conserved and is recognized in the context of a common DP allele, it may represent an important target region for vaccine development. Our results indicate that vaccines containing this epitope may induce in a significant fraction of those immunized CTL active against at least half of all HIV-1 strains. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT MED,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL MOLEC SCI,BALTIMORE,MD 21205. NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. UNIV IOWA,COLL MED,DEPT VET AFFAIRS MED CTR,IOWA CITY,IA 52242. UNIV IOWA,COLL MED,DEPT INTERNAL MED,IOWA CITY,IA 52242. FU NIAID NIH HHS [AI-28108, AI24138, AI-28206] NR 59 TC 67 Z9 67 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 1 PY 1991 VL 146 IS 5 BP 1470 EP 1477 PG 8 WC Immunology SC Immunology GA EY684 UT WOS:A1991EY68400012 PM 1704395 ER PT J AU KING, CL POINDEXTER, RW RAGUNATHAN, J FLEISHER, TA OTTESEN, EA NUTMAN, TB AF KING, CL POINDEXTER, RW RAGUNATHAN, J FLEISHER, TA OTTESEN, EA NUTMAN, TB TI FREQUENCY-ANALYSIS OF IGE-SECRETING B-LYMPHOCYTES IN PERSONS WITH NORMAL OR ELEVATED SERUM IGE LEVELS SO JOURNAL OF IMMUNOLOGY LA English DT Article ID IMMUNOGLOBULIN-E; POKEWEED MITOGEN; INTERFERON-GAMMA; CELLS; INVITRO; INTERLEUKIN-4; CULTURES AB The immunoregulatory mechanisms that determine the high serum IgE antibody levels in disorders such as helminth parasite infections and the hyper-IgE recurrent infection syndrome (HIE) remain poorly understood. To assess whether elevated serum IgE levels result from an increased number of B lymphocytes committed to IgE production, the proportion of IgE-producing B lymphocytes was determined by a filter immunoplaque assay using PBMC from persons with a broad range of serum IgE levels that included normal persons (n = 9) and patients with loiasis (n = 12), tropical pulmonary eosinophilia (TPE) (n = 6), lymphatic filariasis (n = 28), and HIE (n = 8). PBMC from these persons were assessed for production of in vitro IgE. The geometric mean number of IgE-secreting cells in 10(5) B lymphocytes in PBMC was 0.42 (range 0-2.2) in normal persons, 5.6 (range 0.1-35.5) among patients with loiasis, 9.4 (range 0.-53.2) among patients with lymphatic filariasis, 52 (range 31.5-115) among patients with TPE, and 218 (range 56-1404) among patients with HIE. When all study subjects were grouped, there were significant correlations with serum IgE levels (r2 = 0.78; p < 0.0001) and net spontaneous in vitro IgE production (r2 = 0.8; P < 0.0001). Estimates of the amount of IgE production per B lymphocyte were similar among normal persons,, patients with filarial infections, and patients with TPE (geometric means of 134, 96, and 141 pg/ml/cell, respectively); in contrast, for HIE patients, IgE production by individual B cells was significantly lower (geometric mean 28 pg/ml/cell; p < 0.001). These observations demonstrate that clonal expansion of IgE-producing B lymphocytes is a major mechanism underlying the elevated serum IgE levels seen in persons with hyper-IgE states. C1 NIH,DEPT CLIN PATHOL,BETHESDA,MD 20892. ANNA UNIV,DEPT BIOTECHNOL,MADRAS,INDIA. RP KING, CL (reprint author), NIH,PARASIT DIS LAB,BLDG 4,ROOM 126,BETHESDA,MD 20892, USA. NR 17 TC 64 Z9 65 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 1 PY 1991 VL 146 IS 5 BP 1478 EP 1483 PG 6 WC Immunology SC Immunology GA EY684 UT WOS:A1991EY68400013 PM 1899687 ER PT J AU BRISTOL, LA SMITH, MR BHAT, NK DURUM, SK AF BRISTOL, LA SMITH, MR BHAT, NK DURUM, SK TI IL-1 INDUCES ORNITHINE DECARBOXYLASE IN NORMAL LYMPHOCYTES-T SO JOURNAL OF IMMUNOLOGY LA English DT Article ID C-FOS; POLYAMINE BIOSYNTHESIS; MONOCLONAL-ANTIBODY; NUCLEOTIDE-SEQUENCE; PROTEIN-SYNTHESIS; RAPID ACTIVATION; MESSENGER-RNA; CELL-LINE; INTERLEUKIN-1; INDUCTION AB IL-1-alpha regulation of ornithine decarboxylase (ODC) was examined in T cells because IL-1 is a costimulus for T cell proliferation and ODC is a critical enzyme in the metabolic events associated with cellular proliferation. In the present study, we demonstrate that IL-1-alpha induces ODC mRNA and ODC enzyme activity in T cells. Unlike many IL-1 actions on T cells, this did not require a costimulus from the TCR, IL-1 alone being sufficient to induce ODC. The mechanism of IL-1 induction of ODC probably operates at several levels, including transcription, mRNA stability, and translation. Previous studies have shown that IL-1 prepares T cells for replication by increasing the production of c-jun, c-fos, c-myc, growth factors, growth factor receptors, and the response to growth factors. From the present study, ODC induction can be added to the list of IL-1-induced replicative machinery in T cells. C1 NCI,FREDERICK CANC RES FACIL,PROGRAM RESOURCES INC NUCL ACID & PROT LAB,FREDERICK,MD 21701. NCI,FREDERICK CANC RES FACIL,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NCI,FREDERICK CANC RES FACIL,BIOL RESPONSE MODIFIERS PROGRAM,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74102] NR 33 TC 9 Z9 9 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 1 PY 1991 VL 146 IS 5 BP 1509 EP 1515 PG 7 WC Immunology SC Immunology GA EY684 UT WOS:A1991EY68400018 PM 1993840 ER PT J AU MANGAN, DF WELCH, GR WAHL, SM AF MANGAN, DF WELCH, GR WAHL, SM TI LIPOPOLYSACCHARIDE, TUMOR-NECROSIS-FACTOR-ALPHA, AND IL-1-BETA PREVENT PROGRAMMED CELL-DEATH (APOPTOSIS) IN HUMAN PERIPHERAL-BLOOD MONOCYTES SO JOURNAL OF IMMUNOLOGY LA English DT Article ID CENTRIFUGAL ELUTRIATION CCE; ENRICHED FRACTIONS; ACTIVATION; LEADS; ENDONUCLEASE; MACROPHAGES; RECOGNITION; INDUCTION; SUBSETS AB Human peripheral blood monocytes progressively lose viability when cultured in the absence of serum, cytokines, or other stimuli. In this study, we investigated whether monocyte death results from membrane damage (i.e., necrosis) or internally regulated processes [i.e., programmed cell death (PCD) or apoptosis]. Our results clearly indicated that monocytes die by PCD when cultured without stimulation. Death was associated with fragmentation of DNA into integer multiples of approximately 200 bp, a decrease in cell size, condensation of the nucleus and cytoplasmic organelles, and membrane blebbing, all of which are cardinal features of PCD. Monocytes exposed to nonphysiologic conditions such as acidic media (pH 4.2), 56-degrees-C for 30 min, or freezing and thawing were killed without concomitant DNA fragmentation, indicating that DNA fragmentation was not a result of cell death per se. Addition of Escherichia coli LPS, a potent monocyte activating agent, in concentrations as low as 0.1 ng/ml caused a marked increase in monocyte survival and prevented DNA fragmentation. Moreover, exogenous human rTNF-alpha or IL-1-beta also prevented PCD, suggesting that PCD is regulated by certain cytokines released from LPS-stimulated monocytes. The results indicate that in the absence of appropriate stimulation, monocytes are programmed to undergo a sequence of molecular events leading to cell death. Regulation of PCD may be an important homeostatic mechanism for controlling the number of monocytes available to respond to infection, wound healing, and tumor growth. RP MANGAN, DF (reprint author), NIDR,IMMUNOL LAB,CELLULAR IMMUNOL SECT,BLDG 30,RM 329,BETHESDA,MD 20892, USA. FU NIDCR NIH HHS [DE05576] NR 28 TC 340 Z9 349 U1 1 U2 5 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 1 PY 1991 VL 146 IS 5 BP 1541 EP 1546 PG 6 WC Immunology SC Immunology GA EY684 UT WOS:A1991EY68400023 PM 1993844 ER PT J AU CAULADABENEDETTI, Z ALZAMEL, F SHER, A JAMES, S AF CAULADABENEDETTI, Z ALZAMEL, F SHER, A JAMES, S TI COMPARISON OF TH1-ASSOCIATED AND TH2-ASSOCIATED IMMUNE REACTIVITIES STIMULATED BY SINGLE VERSUS MULTIPLE VACCINATION OF MICE WITH IRRADIATED SCHISTOSOMA-MANSONI CERCARIAE SO JOURNAL OF IMMUNOLOGY LA English DT Article ID RADIATION-ATTENUATED CERCARIAE; P-STRAIN MICE; T-HELPER CELL; PROTECTIVE IMMUNITY; MONOCLONAL-ANTIBODIES; CYTOKINE PRODUCTION; INDUCED RESISTANCE; NONLIVING VACCINE; INDUCTION; ANTIGEN AB Mice immunized against Schistosoma mansoni by a single percutaneous exposure to radiation-attenuated parasite larvae demonstrate partial resistance to challenge infection that has been shown to correlate with development of cell-mediated immunity, whereas mice hyperimmunized by multiple exposure to attenuated larvae produce antibodies capable of transferring partial protection to naive recipients. Measurement of Ag-specific lymphokine responses in these animals suggested that the difference in resistance mechanisms may be due to the differential induction of Th subset response by the two immunization protocols. Thus, upon Ag stimulation, singly immunized mice predominantly demonstrated responses associated with Th1 reactivity, including IL-2 and IFN-gamma production, whereas multiply immunized animals showed increased IL-5, IL-4, and IgG1 antibody production associated with enhanced Th2 response. These responses demonstrated some degree of organ compartmentalization, with splenocytes demonstrating higher Th1-related lymphokine production and cells from draining lymph nodes showing stronger proliferation and Th2 type reactivity. However, hyperimmunized mice also continued to demonstrate substantial Th1-associated immune reactivity. Moreover, in vivo Ag challenge elicited activated larvacidal macrophages in hyperimmunized animals. These observations indicate that protective cell-mediated mechanisms associated with induction of CD4+ Th1 cell reactivity predominate in singly vaccinated mice. Further vaccination stimulates Th2 responses, such as enhanced IgG1 production, that may also contribute to protective immunity. C1 NIAID,PARASIT DIS LAB,IMMUNOL & CELL BIOL SECT,BETHESDA,MD 20892. UNIV SAO PAULO,INST CIENCIAS BIOMED,DEPT IMMUNOL,SAO PAULO,BRAZIL. NR 33 TC 88 Z9 88 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 1 PY 1991 VL 146 IS 5 BP 1655 EP 1660 PG 6 WC Immunology SC Immunology GA EY684 UT WOS:A1991EY68400040 PM 1671579 ER PT J AU HOSMALIN, A NARA, PL ZWEIG, M LERCHE, NW CEASE, KB GARD, EA MARKHAM, PD PUTNEY, SD DANIEL, MD DESROSIERS, RC BERZOFSKY, JA AF HOSMALIN, A NARA, PL ZWEIG, M LERCHE, NW CEASE, KB GARD, EA MARKHAM, PD PUTNEY, SD DANIEL, MD DESROSIERS, RC BERZOFSKY, JA TI PRIMING WITH T-HELPER CELL EPITOPE PEPTIDES ENHANCES THE ANTIBODY-RESPONSE TO THE ENVELOPE GLYCOPROTEIN OF HIV-1 IN PRIMATES SO JOURNAL OF IMMUNOLOGY LA English DT Article ID ACQUIRED IMMUNODEFICIENCY SYNDROME; IMMUNE-RESPONSE; SYNTHETIC IMMUNOGEN; ANTIGENIC SITES; VIRUS; PROTEIN; RECOGNITION; AIDS; LYMPHOCYTES; INFECTION AB The induction of a memory immune response to HIV, mediated by any kind of effector mechanism, requires the induction of T cell help. In previous studies performed in different murine MHC haplo-types, three immunodominant T cell epitopes (T1, T2, and TH4.1) had been identified in the HIV envelope glycoprotein. Moreover, these peptides were proliferative T cell epitopes in humans. In this study, rhesus monkeys, Macaca mulatta, were primed with these three peptides either in combination or given separately. Half of the monkeys had a proliferative response to one or more of the priming peptide(s). Those monkeys who had a T cell proliferative response also had a high antibody response after one boost with a suboptimal dose of the native protein gp 160, whereas three of four control monkeys who had received only the native protein immunization gave no detectable antibody response, and one displayed a very weak response. For reasons that are unclear, antibodies only to the gp41 portion of gp 160 could be detected in the sera. Thus, the peptides can prime Th cells in primates for an enhanced antibody response on first exposure to the whole protein. The three peptides belong to highly conserved and nonglycosylated regions of the envelope protein. The fact that the peptides acted as immunogenic T cell proliferative and helper epitopes in nonhuman primates is very encouraging for including them in future vaccine studies in humans. C1 NCI, METAB BRANCH,MOLEC IMMUNOGENET & VACCINE RES SECT, BLDG 10,RM 6B12, BETHESDA, MD 20892 USA. REPLIGEN CORP, CAMBRIDGE, MA 02139 USA. NCI, FREDERICK CANC RES FACIL, TUMOR CELL BIOL LAB, FREDERICK, MD 21701 USA. NCI, FREDERICK CANC RES FACIL, PROGRAM RESOURCES INC, NUCLE ACID & PROT SYNTH LAB, FREDERICK, MD 21701 USA. UNIV CALIF DAVIS, CALIF PRIMATE RES CTR, DAVIS, CA 95616 USA. BIONET RES LABS INC, ROCKVILLE, MD 20850 USA. NEW ENGLAND REG PRIMATE RES CTR, SOUTHBOROUGH, MA 01772 USA. NR 31 TC 34 Z9 34 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 1 PY 1991 VL 146 IS 5 BP 1667 EP 1673 PG 7 WC Immunology SC Immunology GA EY684 UT WOS:A1991EY68400042 PM 1704401 ER PT J AU SANTOSHAM, M LETSON, GW WOLFF, M REID, R GAHAGAN, S ADAMS, R CALLAHAN, C SACK, RB KAPIKIAN, AZ AF SANTOSHAM, M LETSON, GW WOLFF, M REID, R GAHAGAN, S ADAMS, R CALLAHAN, C SACK, RB KAPIKIAN, AZ TI A FIELD-STUDY OF THE SAFETY AND EFFICACY OF 2 CANDIDATE ROTAVIRUS VACCINES IN A NATIVE-AMERICAN POPULATION SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID LINKED IMMUNOSORBENT-ASSAY; YOUNG-CHILDREN; VENEZUELAN INFANTS; RIT-4237; PROTECTION; DIARRHEA; GASTROENTERITIS; IMMUNIZATION; TRIAL; LIVE AB A double-blind, randomized, placebo-controlled trial was conducted to evaluate the safety and efficacy of a rhesus rotavirus vaccine and RIT 4237, a bovine rotavirus vaccine, in a Navajo population. Infants aged 2-5 months were randomized to receive one dose of either 10(4) pfu of the rhesus rotavirus vaccine or 10(8) pfu of the RIT 4237 vaccine or placebo. Eleven (10.2%) of 108 infants in the rhesus vaccine group, 11 (10.4%) of 106 in the RIT 4237 group, and 9 (8.4%) of 107 in the placebo group experienced rotavirus diarrhea during the follow-up period of 17 months. Thus, in this population, neither vaccine was efficacious in preventing rotavirus diarrhea. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT PEDIAT,DIV PEDIAT INFECT DIS,BALTIMORE,MD 21205. NIAID,INFECT DIS LAB,BETHESDA,MD 20892. INDIAN HLTH SERV,NAVAJO AREA OFF,WINDOW ROCK,AZ. RP SANTOSHAM, M (reprint author), JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT INT HLTH,DIV GEOG MED,615 N WOLFE ST,BALTIMORE,MD 21205, USA. FU NIAID NIH HHS [AI-02660] NR 23 TC 76 Z9 79 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD MAR PY 1991 VL 163 IS 3 BP 483 EP 487 PG 5 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA EY495 UT WOS:A1991EY49500009 PM 1995721 ER PT J AU ROILIDES, E WALSH, TJ PIZZO, PA RUBIN, M AF ROILIDES, E WALSH, TJ PIZZO, PA RUBIN, M TI GRANULOCYTE COLONY-STIMULATING FACTOR ENHANCES THE PHAGOCYTIC AND BACTERICIDAL ACTIVITY OF NORMAL AND DEFECTIVE HUMAN NEUTROPHILS SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID CHEMOTHERAPY; NEUTROPENIA; AIDS AB Granulocyte colony-stimulating factor (G-CSF) stimulates proliferation of myeloid cells and may be a valuable adjunct in prevention and treatment of neutropenia-associated infections. Neutrophil (PMNL) phagocytic and microbicidal functions against Staphylococcus aureus and Candida albicans blastoconidia were therefore evaluated. Bacterial phagocytosis and bactericidal activity were significantly enhanced by approximately 50%-70% after preincubation of normal PMNL with G-CSF in concentrations of 1000-4000 units/ml for 10 min at 37-degrees-C. G-CSF in similar concentrations enhanced the defective bactericidal activity of PMNL from HIV-1-infected patients by approximately 70%-150% and reached the baseline control PMNL killing. However, G-CSF enhanced neither phagocytosis nor fungicidal activity of normal PMNL against C. albicans blastoconidia. These data demonstrate that G-CSF enhances the antibacterial but not the antifungal activities of human PMNL in vitro and also improves the defective PMNL bactericidal activity of HIV-1-infected patients. C1 NCI,PEDIAT BRANCH,INFECT DIS SECT,BLDG 10,RM 13N240,BETHESDA,MD 20892. NR 28 TC 271 Z9 272 U1 1 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD MAR PY 1991 VL 163 IS 3 BP 579 EP 583 PG 5 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA EY495 UT WOS:A1991EY49500024 PM 1704903 ER PT J AU SHIH, CC KANESHIMA, H RABIN, L NAMIKAWA, R SAGER, P MCGOWAN, J MCCUNE, JM AF SHIH, CC KANESHIMA, H RABIN, L NAMIKAWA, R SAGER, P MCGOWAN, J MCCUNE, JM TI POSTEXPOSURE PROPHYLAXIS WITH ZIDOVUDINE SUPPRESSES HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION IN SCID-HU MICE IN A TIME-DEPENDENT MANNER SO JOURNAL OF INFECTIOUS DISEASES LA English DT Note ID MOUSE; 3'-AZIDO-3'-DEOXYTHYMIDINE; MODEL; AIDS AB Occupational exposure to the human immunodeficiency virus (HIV) has led to a low but finite incidence of infection among health care providers. In such circumstances, postexposure administration of 3'-azido-3'-deoxythymidine (zidovudine; AZT) might be beneficial. To test this possibility, the SCID-hu mouse (the immunodeficient C.B-17 scid/scid mouse engrafted with human hematolymphoid organs) was treated with AZT at different times after intravenous infection with a standard dose of HIV (known to infect 100% of animals). If given within 2 h, AZT suppressed infection in all animals; if given after 2 days, no suppression was observed. At least in some animals, an AZT-sensitive phase lasted for as long as 36 h. These data support the hypothesis that prompt administration of AZT might be efficacious in suppressing acute HIV infection in humans. Further studies in the SCID-hu mouse might provide insight into treatment protocols of even greater efficacy. C1 NIAID,DIV AIDS,BETHESDA,MD 20892. RP SHIH, CC (reprint author), SYSTEMIX INC,HIV GRP,3400 W BAYSHORE RD,PALO ALTO,CA 94303, USA. FU NIAID NIH HHS [AI-29323] NR 15 TC 91 Z9 91 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD MAR PY 1991 VL 163 IS 3 BP 625 EP 627 PG 3 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA EY495 UT WOS:A1991EY49500032 PM 1995734 ER PT J AU AUSTEN, KF AF AUSTEN, KF TI TOWARD A STANDARD EVALUATION FOR DATA SHARING AND PROGNOSIS - GENERAL DISCUSSION SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Discussion RP AUSTEN, KF (reprint author), NIAID,CLIN INVEST LAB,MAST CELL PHYSIOL SECT,BETHESDA,MD 20892, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD MAR PY 1991 VL 96 IS 3 BP S60 EP S63 DI 10.1111/1523-1747.ep12469487 PG 4 WC Dermatology SC Dermatology GA FC839 UT WOS:A1991FC83900033 ER PT J AU KETTELHUT, BV METCALFE, DD AF KETTELHUT, BV METCALFE, DD TI PEDIATRIC MASTOCYTOSIS SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Article; Proceedings Paper CT INTERDISCIPLINARY ROUNDTABLE DISCUSSION ON CLINICAL ADVANCES IN MASTOCYTOSIS CY JUN 15, 1990 CL SOUTHAMPTON, BERMUDA ID BULLOUS URTICARIA PIGMENTOSA; CUTANEOUS MASTOCYTOSIS; DISODIUM-CROMOGLYCATE; MAST-CELL; HISTAMINE; KETOTIFEN; CHILDREN AB The onset of mastocytosis occurs between birth and 2 years of age in approximately 55% of all cases; an additional 10% develop the disease before the age of 15 years. Mastocytosis in these age groups differs in many respects from mastocytosis that has its onset in adulthood. The typical presentation of pediatric-onset mastocytosis consists of cutaneous manifestations: either a solitary mastocytoma, urticaria pigmentosa, or, less commonly, diffuse cutaneous mastocytosis. Particularly in infants, bullous eruptions may occur. Mastocytosis in infants and children may involve internal organs, including the bone marrow and the gastrointestinal tract, although such manifestations appear to be less common in children than in adults. Plasma histamine levels may be elevated in pediatriconest mastocytosis. Treatment usually involves the use of H1 and H2 antihistamines to control itching and to control the hypersecretion of gastric acid that may occur. The prognosis for children with mast cell disease is variable; approximately half of the children with urticaria pigmentosa may experience resolution of lesions and symptoms by adolescence. C1 CHILDRENS HOSP MED CTR,DIV ALLERGY & CLIN IMMUNOL,ELLAND & BETHESDA AVE,CINCINNATI,OH 45229. NIAID,CLIN INVEST LAB,MAST CELL PHYSIOL SECT,BETHESDA,MD 20892. NR 40 TC 82 Z9 84 U1 0 U2 1 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD MAR PY 1991 VL 96 IS 3 BP S15 EP S18 DI 10.1111/1523-1747.ep12468942 PG 4 WC Dermatology SC Dermatology GA FC839 UT WOS:A1991FC83900024 PM 1705949 ER PT J AU METCALFE, DD AF METCALFE, DD TI THE LIVER, SPLEEN, AND LYMPH-NODES IN MASTOCYTOSIS SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Article; Proceedings Paper CT INTERDISCIPLINARY ROUNDTABLE DISCUSSION ON CLINICAL ADVANCES IN MASTOCYTOSIS CY JUN 15, 1990 CL SOUTHAMPTON, BERMUDA AB In systemic mastocytosis the liver, spleen, and lymph nodes may be infiltrated by mast cells, with patterns of infiltration specific for each tissue. This may result in hepatosplenomegaly and enlarged lymph nodes. Extensive involvement with mast cells may also be associated with organ dysfunction. Specifically, in the case of liver, mast cell infiltration may result in fibrosis, portal hypertension, and abdominal ascites. Clinically significant involvement of the liver, spleen, and lymph nodes appears to be more common in patients with aggressive forms of mastocytosis, including those with a hematologic disorder. RP METCALFE, DD (reprint author), NIAID,CLIN INVEST LAB,MAST CELL PHYSIOL SECT,BLDG 10,ROOM 11C210,BETHESDA,MD 20892, USA. NR 3 TC 44 Z9 45 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD MAR PY 1991 VL 96 IS 3 BP S45 EP S46 DI 10.1111/1523-1747.ep12469022 PG 2 WC Dermatology SC Dermatology GA FC839 UT WOS:A1991FC83900029 PM 2002262 ER PT J AU METCALFE, DD AF METCALFE, DD TI CLINICAL ADVANCES IN MASTOCYTOSIS - AN INTERDISCIPLINARY ROUND-TABLE DISCUSSION - SOUTHAMPTON, BERMUDA JUNE 15, 1990 - CONCLUSIONS SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Editorial Material RP METCALFE, DD (reprint author), NIAID,CLIN INVEST LAB,MAST CELL PHYSIOL SECT,BLDG 10,ROOM 11C210,BETHESDA,MD 20892, USA. NR 0 TC 27 Z9 27 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD MAR PY 1991 VL 96 IS 3 BP S64 EP S65 DI 10.1111/1523-1747.ep12469665 PG 2 WC Dermatology SC Dermatology GA FC839 UT WOS:A1991FC83900034 ER PT J AU METCALFE, DD AF METCALFE, DD TI CLASSIFICATION AND DIAGNOSIS OF MASTOCYTOSIS - CURRENT STATUS SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Article; Proceedings Paper CT INTERDISCIPLINARY ROUNDTABLE DISCUSSION ON CLINICAL ADVANCES IN MASTOCYTOSIS CY JUN 15, 1990 CL SOUTHAMPTON, BERMUDA ID SYSTEMIC MASTOCYTOSIS; BONE-MARROW; MAST-CELLS; ANAPHYLAXIS; HISTAMINE; DISEASE; GROWTH AB Mastocytosis is a disease characterized by an abnormal increase in mast cells. Manifestations of the disease are provoked in large part by the resultant increase in mast cell-derived mediators, which have a variety of local and systemic effects. Mastocytosis is variable in respect to the organ systems involved, clinical manifestations, and association with hematologic diseases. This has suggested the need for an improved classification scheme to allow assessment of prognosis and therapy. The heterogeneity of the disease patterns in mastocytosis strongly suggests that more than one biologic lesion may occur in the developmental sequence that leads to placement of mature mast cells in tissues. RP METCALFE, DD (reprint author), NIAID,CLIN INVEST LAB,MAST CELL PHYSIOL SECT,BLDG 10,ROOM 11C210,BETHESDA,MD 20892, USA. NR 11 TC 124 Z9 132 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD MAR PY 1991 VL 96 IS 3 BP S2 EP S2 DI 10.1111/1523-1747.ep12468882 PG 1 WC Dermatology SC Dermatology GA FC839 UT WOS:A1991FC83900022 PM 2002248 ER PT J AU METCALFE, DD AF METCALFE, DD TI CLINICAL ADVANCES IN MASTOCYTOSIS - AN INTERDISCIPLINARY ROUND-TABLE DISCUSSION - SOUTHAMPTON, BERMUDA JUNE 15, 1990 - INTRODUCTION SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Editorial Material RP METCALFE, DD (reprint author), NIAID,CLIN INVEST LAB,MAST CELL PHYSIOL SECT,BLDG 10,ROOM 11C21,BETHESDA,MD 20892, USA. NR 0 TC 30 Z9 30 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD MAR PY 1991 VL 96 IS 3 BP S1 EP S1 DI 10.1111/1523-1747.ep12468869 PG 1 WC Dermatology SC Dermatology GA FC839 UT WOS:A1991FC83900021 ER PT J AU METCALFE, DD AF METCALFE, DD TI THE TREATMENT OF MASTOCYTOSIS - AN OVERVIEW SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Article; Proceedings Paper CT INTERDISCIPLINARY ROUNDTABLE DISCUSSION ON CLINICAL ADVANCES IN MASTOCYTOSIS CY JUN 15, 1990 CL SOUTHAMPTON, BERMUDA ID ASCITES AB The treatment of mastocytosis requires a recognition of specific disease patterns of involvement, with consequent institution of appropriate therapy based on the disease pattern manifested in a given patient. Treatment for most forms of mastocytosis is conservative and symptomatic. H1 and H2 antihistamines in combination or alone remain the primary drugs of choice. In specific cases, patients may require aspirin and/or steroids; some must be prepared to self-administer epinephrine for severe anaphylactic episodes. In patients with associated hematologic disorders, the treatment of the disorder will depend on the hematologic findings. In rare cases, and in aggressive forms of mastocytosis only, it may be necessary to consider limited forms of chemotherapy. RP METCALFE, DD (reprint author), NIAID,CLIN INVEST LAB,MAST CELL PHYSIOL SECT,BLDG 10,ROOM 11C210,BETHESDA,MD 20892, USA. NR 4 TC 69 Z9 69 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD MAR PY 1991 VL 96 IS 3 BP S55 EP S59 DI 10.1111/1523-1747.ep12469049 PG 5 WC Dermatology SC Dermatology GA FC839 UT WOS:A1991FC83900032 PM 2002266 ER PT J AU PARKER, RI AF PARKER, RI TI HEMATOLOGIC ASPECTS OF MASTOCYTOSIS .1. BONE-MARROW PATHOLOGY IN ADULT AND PEDIATRIC SYSTEMIC MAST-CELL DISEASE SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Article; Proceedings Paper CT INTERDISCIPLINARY ROUNDTABLE DISCUSSION ON CLINICAL ADVANCES IN MASTOCYTOSIS CY JUN 15, 1990 CL SOUTHAMPTON, BERMUDA AB The typical bone marrow lesions seen in adults with systemic mast cell disease (SMCD) are foci of spindle-shaped mast cells in a fibrotic matrix and are found in up to 90% of adults with SMCD. Lymphocytes and eosinophils frequently are admixed with the mast cells, forming the classic MEL lesion. The mast cell lesions can be found in pervascular, peritrabecular, or intertrabecular locations and may on occasion completely replace intratrabecular regions of the marrow. In contrast, the mast cell lesions found in children with cutaneous mast cell disorders are uniformly small and subtle and are most frequently located perivascularly. Lymphocytes, eosinophils, and early myeloid elements may be associated with these lesions. Of perhaps greater specificity for SMCD is the finding of confluent clusters of mast cells on the marrow aspirates; such clusters are noted in up to 30% of patients with SMCD. RP PARKER, RI (reprint author), NIH,DEPT CLIN PATHOL,HEMATOL SERV,BLDG 10,ROOM 2C-390,BETHESDA,MD 20892, USA. NR 6 TC 41 Z9 41 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD MAR PY 1991 VL 96 IS 3 BP S47 EP S51 DI 10.1111/1523-1747.ep12469034 PG 5 WC Dermatology SC Dermatology GA FC839 UT WOS:A1991FC83900030 PM 2002263 ER PT J AU PARKER, RI AF PARKER, RI TI HEMATOLOGIC ASPECTS OF MASTOCYTOSIS .2. MANAGEMENT OF HEMATOLOGIC DISORDERS IN ASSOCIATION WITH SYSTEMIC MAST-CELL DISEASE SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Article; Proceedings Paper CT INTERDISCIPLINARY ROUNDTABLE DISCUSSION ON CLINICAL ADVANCES IN MASTOCYTOSIS CY JUN 15, 1990 CL SOUTHAMPTON, BERMUDA AB Individuals with systemic mast cell disease (SMCD) may develop various hematologic abnormalities, including cytopenias, myeloproliferative or myelodysplastic syndromes, lymphoproliferative syndromes, and primary or secondary leukemias. Management of those patients is often complicated by their associated hematologic abnormalities. In the case of non-malignant hematologic syndromes, the approach to management is supportive. At present, overt malignancies are managed with traditional chemotherapy. The presence of leukemia in patients with mast cell disease usually indicates a grave prognosis. RP PARKER, RI (reprint author), NIH,DEPT CLIN PATHOL,HEMATOL SERV,BLDG 10,ROOM 2C-390,BETHESDA,MD 20892, USA. NR 7 TC 34 Z9 34 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD MAR PY 1991 VL 96 IS 3 BP S52 EP S54 DI 10.1111/1523-1747.ep12469045 PG 3 WC Dermatology SC Dermatology GA FC839 UT WOS:A1991FC83900031 PM 2002265 ER PT J AU LEONARD, EJ YOSHIMURA, T ROT, A NOER, K WALZ, A BAGGIOLINI, M WALZ, DA GOETZL, EJ CASTOR, CW AF LEONARD, EJ YOSHIMURA, T ROT, A NOER, K WALZ, A BAGGIOLINI, M WALZ, DA GOETZL, EJ CASTOR, CW TI CHEMOTACTIC ACTIVITY AND RECEPTOR-BINDING OF NEUTROPHIL ATTRACTANT ACTIVATION PROTEIN-1 (NAP-1) AND STRUCTURALLY RELATED HOST DEFENSE CYTOKINES - INTERACTION OF NAP-2 WITH THE NAP-1 RECEPTOR SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Article DE PLATELET FACTOR-IV; PF-4; CONNECTIVE TISSUE ACTIVATING PEPTIDE-III; CTAP-III; INTERLEUKIN-8; PLATELET; FLOW CYTOMETRY ID PLATELET FACTOR 4; STIMULATION; EXPRESSION; SEQUENCE AB Neutrophil attractant/activation protein-1 (NAP-1) has sequence similarity to platelet factor-4 (PF-4) and to NAP-2 (a truncated from of connective tissue activating protein-III [CTAP-III(des 1-15)]. We compared chemotactic activity for neutrophils of these related proteins. We also included for comparison CTAP-III(des 1-13), the C-terminal dodecapeptide of PF-4 [PF-4(59-70)], and C5a. Chemotactic potency (EC50) was highest for NAP-1 and C5a. Although chemotactic efficacy (peak percentage of neutrophils migrating) was comparable for C5a, NAP-1, and NAP-2, the NAP-2 response occurred only at concentrations 100-fold higher than the NAP-1 EC50 of 10(-8) M. Data for the CTAP-III proteins confirmed that CTAP-III is not an attractant and that chemotactic activity appears as a result of cleavage of residues at the N-terminus to make CTAP-III(des 1-13) or NAP-2 [CTAP-III(des 1-15)]. Chemotactic activity of PF-4 was low and variable, with no significant response by neutrophils from six of nine subjects. In contrast, PF-4(59-70) regularly induced high chemotactic responses, although the EC50 of 1.6 x 10(-5) M was 1,000-fold greater than that of NAP-1. The binding of fluoresceinted NAP-1 to neutrophils was inhibited by unlabeled NAP-1 or NAP-2 but not by PF-4 or PF-4 (59-70). This suggests that NAP-2 interacts with the neutrophil NAP-1 receptor. Despite the low chemotactic potency of NAP-2, it is a potential attractant at sites of injury because of the relatively large amounts of the parent CTAP-III released from platelets, as indicated by a serum concentration of approximately 10(-6) M. C1 SANDOZ GMBH,A-1235 VIENNA,AUSTRIA. UNIV BERN,THEODOR KOCHER INST,CH-3001 BERN,SWITZERLAND. WAYNE STATE UNIV,SCH MED,DEPT PHYSIOL,DETROIT,MI 48201. PROGRAM RESOURCES INC,FREDERICK,MD. UNIV CALIF SAN FRANCISCO,SCH MED,DEPT MED,SAN FRANCISCO,CA 94143. UNIV MICHIGAN,SCH MED,DEPT INTERNAL MED,ANN ARBOR,MI 48104. RP LEONARD, EJ (reprint author), NCI,FREDERICK CANC RES FACIL,IMMUNOBIOL LAB,IMMUNOPATHOL SECT,BLDG 560,ROOM 12-71,FREDERICK,MD 21701, USA. FU NHLBI NIH HHS [HL 27073] NR 29 TC 67 Z9 67 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PD MAR PY 1991 VL 49 IS 3 BP 258 EP 265 PG 8 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA EX542 UT WOS:A1991EX54200006 PM 1997632 ER PT J AU CARROLL, FI LEWIN, AH ABRAHAM, P PARHAM, K BOJA, JW KUHAR, MJ AF CARROLL, FI LEWIN, AH ABRAHAM, P PARHAM, K BOJA, JW KUHAR, MJ TI SYNTHESIS AND LIGAND-BINDING OF COCAINE ISOMERS AT THE COCAINE RECEPTOR SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID DOPAMINE UPTAKE SITES; H-3 COCAINE; NONHUMAN-PRIMATES; STRIATUM; PUTAMEN; MOUSE; DRUGS AB The cocaine binding site at the dopamine transporter has been found to be stereoselective. Thus, the seven possible stereoisomers of (-)-cocaine have been synthesized and found to inhibit [H-3]-2-beta-carbomethoxy-3-beta-(4-fluorophenyl)tropane ([H-3]WIN 35,428) with potencies ranging from 1/60 to 1/600 of that of (-)-cocaine. The synthesis and characterization of all new compounds is presented. C1 NIDA,ADDICT RES CTR,NEUROSCI BRANCH,BALTIMORE,MD 21224. RP CARROLL, FI (reprint author), RES TRIANGLE INST,POB 12194,RES TRIANGLE PK,NC 27709, USA. FU NIDA NIH HHS [DA05477] NR 19 TC 75 Z9 76 U1 1 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD MAR PY 1991 VL 34 IS 3 BP 883 EP 886 DI 10.1021/jm00107a003 PG 4 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA FB469 UT WOS:A1991FB46900003 PM 2002468 ER PT J AU ADEJARE, A NIE, JY HEBEL, D BRACKETT, LE CHOI, O GUSOVSKY, F PADGETT, WL DALY, JW CREVELING, CR KIRK, KL AF ADEJARE, A NIE, JY HEBEL, D BRACKETT, LE CHOI, O GUSOVSKY, F PADGETT, WL DALY, JW CREVELING, CR KIRK, KL TI EFFECT OF FLUORINE SUBSTITUTION ON THE ADRENERGIC PROPERTIES OF 3-(TERT-BUTYLAMINO)-1-(3,4-DIHYDROXYPHENOXY)-2-PROPANOL SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID CONFORMATION; BINDING; AGENTS AB The 2- and 6-fluoro derivatives of the potent beta-adrenergic agonist 3-(tert-butylamino)-1-(3,4-dihydroxyphenoxy)-2-propanol were prepared and their adrenergic properties examined. The order of potency was as follows: beta-adrenergic activity (simulation of cyclic AMP formation in C6 glioma cells), 2-F = parent >> 6-F; beta-1-activity (rate of contraction, guinea pig atria), parent > 2-F >> 6-F; beta-2-activity (relaxation of guinea pig tracheal strip), 2-F > parent >> 6-F. The affinity of the 2-fluoro analogue for beta-1-adrenergic receptors (inhibition of the specific binding of [H-3]dihydroalprenolol, rat cerebral cortical membranes) was 2 times greater, while the 6-fluoro analogue was 1450 times less than the parent. These results suggest that the aromatic rings of phenoxypropanolamine adrenergic agonists and phenylethanolamine adrenergic agonists bind in similar fashion to the adrenergic receptor, and that if interactions between fluorine and the side-chain hydroxyl group are critical in defining beta-adrenergic selectivity, the interactions are similar in both phenoxypropanolamines and phenylethanolamines. C1 NIDDKD,BIOORGAN CHEM LAB,BETHESDA,MD 20892. NR 17 TC 12 Z9 12 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD MAR PY 1991 VL 34 IS 3 BP 1063 EP 1068 DI 10.1021/jm00107a027 PG 6 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA FB469 UT WOS:A1991FB46900027 PM 1672155 ER PT J AU BRADBURY, BJ BAUMGOLD, J PAEK, R KAMMULA, U ZIMMET, J JACOBSON, KA AF BRADBURY, BJ BAUMGOLD, J PAEK, R KAMMULA, U ZIMMET, J JACOBSON, KA TI MUSCARINIC RECEPTOR-BINDING AND ACTIVATION OF 2ND MESSENGERS BY SUBSTITUTED N-METHYL-N-[4-(1-AZACYCLOALKYL)-2-BUTYNYL]ACETAMIDES SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID PHARMACOLOGICAL PROPERTIES; CHOLINERGIC AGONISTS; SUBTYPES; OXOTREMORINE; CYCLASE; GENES AB A series of substituted azacycloalkyl analogues of the muscarinic agonist UH 5 (N-methyl-N-[4-(1-pyrrolidinyl)-2-butynyl]acetamide, 1a) were synthesized and evaluated pharmacologically. These compounds were developed as intermediates for further derivatization leading to functionalized congeners of 1a. The compounds were synthesized by using a Mannich-type condensation of N-acetyl-N-methylpropargylamine to various substituted saturated azaheterocycles. The compounds were screened at a single concentration in competitive binding assays in rat cerebral cortical membranes against either [H-3]N-methylscopolamine (at 100-mu-M) or [H-3]oxotremorine-M (at 1-mu-M) labels. Candidates were then selected for further evaluation of their effect on phosphoinositide (PI) turnover in membranes from A9L cells transfected with cDNA of either m1-muscarinic cholinergic receptors (m1AChRs) or m3AChRs. The analogues were also tested for the inhibition of adenylate cyclase in NG108-15 cells expressing m4AChRs. The azetidine analogue of 1a had a K(i) value of 12 nM for the inhibition of [H-3]oxotremorine-M binding in rat brain and had an agonist potency at m1-, m3-, and m4AChRs comparable to 1a. The substituted 5- and 6-member ring analogues generally had lower binding affinities and were less potent than 1a in stimulating PI turnover. Several compounds were moderately effective in inhibiting cyclic AMP production in NG108-15 cells. C1 NIDDK, BIOORGAN CHEM LAB, BLDG 8A, ROOM B1A-17, BETHESDA, MD 20892 USA. GEORGE WASHINGTON UNIV, DEPT RADIOL, WASHINGTON, DC 20037 USA. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z01 DK031117-20] NR 26 TC 12 Z9 12 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD MAR PY 1991 VL 34 IS 3 BP 1073 EP 1079 DI 10.1021/jm00107a029 PG 7 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA FB469 UT WOS:A1991FB46900029 PM 1848294 ER PT J AU CORCELLI, A TURNER, RJ AF CORCELLI, A TURNER, RJ TI ROLE OF PHOSPHOLIPIDS IN THE BINDING OF BUMETANIDE TO THE RABBIT PAROTID NA/K/CL COTRANSPORTER SO JOURNAL OF MEMBRANE BIOLOGY LA English DT Article DE LOOP DIURETICS; EXOCRINE GLAND; FLUID SECRETION; LIPID; ACINAR CELL; ION TRANSPORT; CHLORIDE SECRETION; DETERGENT ID K-CL COTRANSPORT; SARCOPLASMIC-RETICULUM; EPITHELIAL TISSUES; TRANSPORT-SYSTEM; H-3 BUMETANIDE; DOG KIDNEY; MEMBRANES; PROTEIN; SECRETION; VESICLES AB It was recently reported (Turner, R.J., George, J.N., 1990, J. Membrane Biol. 113:203-210) that the high affinity bumetanide binding site of the rabbit parotid Na/K/Cl cotransporter could be extracted from a basolateral membrane preparation from this gland using relatively low concentrations of the non-ionic detergent Triton X-100. At the detergent:protein ratios required for complete membrane solubilization bumetanide binding activity in this extract was lost but could be recovered by the addition of crude soybean lipids. In the present paper the ability of various purified lipids to restore high affinity bumetanide binding activity in detergent solubilized rabbit parotid basolateral membranes is studied. We show that the effect of exogenous lipid on the detergent-inactivated bumetanide binding site is to increase the affinity of binding without affecting the number of binding sites. Of the 11 lipid species tested, several relatively minor, negatively charged membrane phospholipids are the most effective in restoring binding activity (phosphatidylserine almost-equal-to phosphatidylglycerol > phosphatidylinositol > cardiolipin), while the major mammalian plasma membrane lipid components phosphatidylcholine, phosphatidylethanolamine, sphingomyelin and cholesterol are without effect. In addition, we show that in the presence of these minor lipids the affinity of bumetanide binding is considerably increased over that observed in the native membrane (e.g., K(d) almost-equal-to 0.06-mu-M in membranes extracted with 0.3% Triton and treated with 0.15% wt/vol phosphatidylserine, vs. K(d) almost-equal-to 3-mu-M in native basolateral membranes). This dramatic dependence of bumetanide binding affinity on the presence of certain lipid species suggests that the properties of the bumetanide binding protein in situ may be quite dependent on the minor lipid content of the plasma membrane. This effect may account for the relatively large variations in bumetanide binding affinity observed from tissue to tissue. C1 NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892. RP CORCELLI, A (reprint author), UNIV BARI,IST FISIOL GEN,I-70124 BARI,ITALY. OI Corcelli, Angela/0000-0001-9658-8520 NR 33 TC 2 Z9 2 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0022-2631 J9 J MEMBRANE BIOL JI J. Membr. Biol. PD MAR PY 1991 VL 120 IS 2 BP 125 EP 130 DI 10.1007/BF01872395 PG 6 WC Biochemistry & Molecular Biology; Cell Biology; Physiology SC Biochemistry & Molecular Biology; Cell Biology; Physiology GA FA241 UT WOS:A1991FA24100004 PM 2072383 ER PT J AU CORR, L BURNSTOCK, G POOLEWILSON, P AF CORR, L BURNSTOCK, G POOLEWILSON, P TI MAGNESIUM INHIBITS THE RESPONSES TO NEUROPEPTIDE-Y IN THE RABBIT CORONARY-ARTERY SO JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY LA English DT Note DE MAGNESIUM; NEUROPEPTIDE-Y; CORONARY ARTERY ID BLOOD-VESSELS; VASOCONSTRICTION; SULFATE; ANGINA C1 UCL, DEPT ANAT, GOWER ST, LONDON WC1, ENGLAND. UCL, CTR NEUROSCI, LONDON WC1, ENGLAND. NHLBI, BETHESDA, MD 20892 USA. NR 19 TC 2 Z9 2 U1 0 U2 0 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2828 EI 1095-8584 J9 J MOL CELL CARDIOL JI J. Mol. Cell. Cardiol. PD MAR PY 1991 VL 23 IS 3 BP 231 EP 235 DI 10.1016/0022-2828(91)90059-U PG 5 WC Cardiac & Cardiovascular Systems; Cell Biology SC Cardiovascular System & Cardiology; Cell Biology GA FJ422 UT WOS:A1991FJ42200001 PM 1880809 ER PT J AU FERRONI, C HANO, O VENTURA, C LAKATTA, EG KLOCKOW, M SPURGEON, H CAPOGROSSI, MC AF FERRONI, C HANO, O VENTURA, C LAKATTA, EG KLOCKOW, M SPURGEON, H CAPOGROSSI, MC TI A NOVEL POSITIVE INOTROPIC SUBSTANCE ENHANCES CONTRACTILITY WITHOUT INCREASING THE CA-2+ TRANSIENT IN RAT MYOCARDIUM SO JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY LA English DT Article DE CARDIOTONIC DRUGS; THIADIAZINONE; EMD-53998; INDO-1; EXCITATION CONTRACTION COUPLING ID STAGE HEART-FAILURE; CARDIAC MYOCYTES; CALCIUM; MUSCLE; RESPONSIVENESS; SENSITIVITY; PIMOBENDAN; MILRINONE; MECHANISM; TISSUES C1 NIA, GERONTOL RES CTR, CARDIOVASC SCI LAB, 4940 EASTERN AVE, BALTIMORE, MD 21224 USA. E MERCK AG, W-6100 DARMSTADT 1, GERMANY. NR 21 TC 47 Z9 47 U1 0 U2 2 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2828 EI 1095-8584 J9 J MOL CELL CARDIOL JI J. Mol. Cell. Cardiol. PD MAR PY 1991 VL 23 IS 3 BP 325 EP 331 DI 10.1016/0022-2828(91)90068-W PG 7 WC Cardiac & Cardiovascular Systems; Cell Biology SC Cardiovascular System & Cardiology; Cell Biology GA FJ422 UT WOS:A1991FJ42200010 PM 1880816 ER PT J AU WISTOW, G KIM, H AF WISTOW, G KIM, H TI LENS PROTEIN EXPRESSION IN MAMMALS - TAXON-SPECIFICITY AND THE RECRUITMENT OF CRYSTALLINS SO JOURNAL OF MOLECULAR EVOLUTION LA English DT Article DE LENS; CRYSTALLINS; MACROSCELIDS; MARSUPIALS; PROTEIN EVOLUTION; ENZYMES ID AMINO-ACID-SEQUENCE; ALPHA-CRYSTALLIN; GAMMA-CRYSTALLIN; ALCOHOL DEHYDROGENASES; LACTATE-DEHYDROGENASE; EVOLUTIONARY STRATEGY; NUCLEOTIDE-SEQUENCE; STRUCTURAL PROTEINS; ZETA-CRYSTALLIN; GUINEA-PIG AB Vertebrate lenses show remarkably taxon-specific patterns of protein composition, most obviously in the recruitment of enzymes as major crystallins. Phylogenetic relationships are particularly apparent in mammals. Here we describe eta-crystallin, which is probably identical to cytosolic aldehyde dehydrogenase, lens-specifically expressed at high abundance in the elephant shrews, primitive eutherians of the family Macroscelidae, and mu-crystallin, a novel lens protein expressed in some marsupials. We have also observed that enzymes that have been recruited as crystallins in some species are also moderately abundant in the lenses of other species. This hints that the origins of enzyme-crystallins may lie in a pool of enzymes widely expressed in lenses at fairly high levels, perhaps because they have important developmental or functional roles in the tissue. RP WISTOW, G (reprint author), NEI,MOLEC & DEV BIOL LAB,ROOM 204,BLDG 6,BETHESDA,MD 20892, USA. NR 45 TC 69 Z9 69 U1 1 U2 3 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0022-2844 J9 J MOL EVOL JI J. Mol. Evol. PD MAR PY 1991 VL 32 IS 3 BP 262 EP 269 DI 10.1007/BF02342749 PG 8 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA EZ813 UT WOS:A1991EZ81300009 PM 1904503 ER PT J AU DARDEN, T JOHNSON, P SMITH, H AF DARDEN, T JOHNSON, P SMITH, H TI MULTI - A SHARED MEMORY APPROACH TO COOPERATIVE MOLECULAR MODELING SO JOURNAL OF MOLECULAR GRAPHICS LA English DT Article; Proceedings Paper CT WORKSHOP ON DESKTOP MOLECULAR VISUALIZATION CY AUG 23-25, 1990 CL BETHESDA, MD SP MOLEC GRAPH SOC AMERICAS DE SHARED MEMORY; PARALLEL PROCESSING; MOLECULAR MODELING ID RIBBON AB A general purpose molecular modeling system, MULTI, based on the UNIX shared memory and semaphore facilities for interprocess communication is described. In addition to the normal querying or monitoring of geometric data, MULTI also provides processes for manipulating conformations, and for displaying peptide or nucleic acid ribbons, Connolly surfaces, close nonbonded contacts, crystal-symmetry related images, least-squares superpositions, and so forth. This paper outlines the basic techniques used in MULTI to ensure cooperation among these specialized processes, and then describes how they can work together to provide a flexible modeling environment. RP DARDEN, T (reprint author), NIEHS,RES TRIANGLE PK,NC 27709, USA. NR 10 TC 3 Z9 3 U1 0 U2 0 PU BUTTERWORTH-HEINEMANN PI WOBURN PA 225 WILDWOOD AVE #UNITB PO BOX 4500, WOBURN, MA 01801-2084 SN 0263-7855 J9 J MOL GRAPHICS JI J. Mol. Graph. PD MAR PY 1991 VL 9 IS 1 BP 18 EP 23 DI 10.1016/0263-7855(91)80028-X PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Computer Science, Interdisciplinary Applications; Crystallography; Mathematical & Computational Biology SC Biochemistry & Molecular Biology; Computer Science; Crystallography; Mathematical & Computational Biology GA FA753 UT WOS:A1991FA75300004 PM 2018752 ER PT J AU GARDNER, DL LEIBENLUFT, E OLEARY, KM COWDRY, RW AF GARDNER, DL LEIBENLUFT, E OLEARY, KM COWDRY, RW TI SELF-RATINGS OF ANGER AND HOSTILITY IN BORDERLINE PERSONALITY-DISORDER SO JOURNAL OF NERVOUS AND MENTAL DISEASE LA English DT Article ID DEXAMETHASONE SUPPRESSION TEST; DIAGNOSTIC INTERVIEW; DEPRESSED-PATIENTS; INPATIENTS; VALIDATION; FEATURES; VALIDITY AB Forty-six patients with borderline personality disorder with and without major depression and 27 normal volunteers completed the Buss-Durkee Hostility Inventory, a self-rating scale of anger and hostility. The patients with borderline personality had significantly higher scores than the normal volunteers. The scores of the patients with borderline personality disorders were not related to gender, treatment or research setting, the degree of acute distress, or the presence of major depression. These findings suggest that a proneness to anger and hostility are enduring characteristics of borderline personality disorder and that anger and depression may represent independent clinical conditions with independent biological mechanisms regulating these two affective states. C1 GEORGETOWN UNIV HOSP,DEPT PSYCHIAT,WASHINGTON,DC 20007. RP GARDNER, DL (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,WASHINGTON,DC 20032, USA. NR 22 TC 45 Z9 45 U1 0 U2 7 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3018 J9 J NERV MENT DIS JI J. Nerv. Ment. Dis. PD MAR PY 1991 VL 179 IS 3 BP 157 EP 161 DI 10.1097/00005053-199103000-00008 PG 5 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA FB638 UT WOS:A1991FB63800008 PM 1997664 ER PT J AU FINK, DW MIRKIN, BL AF FINK, DW MIRKIN, BL TI EFFECT OF NERVE GROWTH-FACTOR ON C-1300 MURINE NEUROBLASTOMA TUMOR-GROWTH AND CATECHOLAMINE CONTENT IN NEONATALLY SYMPATHECTOMIZED MICE SO JOURNAL OF NEUROSCIENCE RESEARCH LA English DT Article DE 6-HYDROXYDOPAMINE; INSITU NEUROBLASTOMA GROWTH; TUMOR CATECHOLAMINE CONCENTRATION; ADRENERGIC NERVOUS SYSTEM-NGF INTERACTION; NGF RECEPTORS ID HUMAN NEURO-BLASTOMA; N-MYC; HYDROXYLASE ACTIVITY; FACTOR NGF; CELL LINE; DIFFERENTIATION; IDENTIFICATION; ANTIBODIES; MECHANISM; CULTURE AB The in situ C-1300 murine neuroblastoma (NMB) tumor model was used to investigate the influence of exogenously administered nerve growth factor (NGF) on tumor growth and tissue catecholamine concentration in mice sympathectomized with 6-hydroxydopamine (6-OHDA) on postnatal days 4-10. Mice were implanted with 1 x 10(6) disaggregated MNB cells 3 days after termination of 6-OHDA administration. NGF (12-15-mu-g/mouse/day) treatment was initiated at the time of MNB cell implantation and continued until sacrifice of the animal. The time interval between tumor cell implantation and detection of palpable tumor (tumor onset time), transverse tumor diameter, tumor weight, tumor weight to body weight ratio, and tumor catecholamine concentration were determined. Neonatal sympathectomy caused a decrease in myocardial norepinephrine concentration of 88% compared with vehicle-treated animals as well as a significant reduction in total body and organ weight. Average body, brain, heart, and spleen weights were decreased 31%, 16%, 25%, and 42%, respectively, below control values. The daily injection of NGF, from the time of MNB tumor implantation to sacrifice, did not prevent these effects of chemical sympathectomy from being expressed. Tumor onset time following implantation of MNB cells was significantly increased in neonatally sympathectomized mice and was not altered by treatment with NGF. In contrast, the decrease in MNB tumor growth rate observed in sympathectomized mice was reversed by administration of NGF. Mean tumor weight and mean tumor to body weight ratio were 89% and 115% of comparable control values, respectively, in sympathectomized mice receiving exogenous NGF. The norepinephrine (NE) and dopamine (DA) concentrations of tumors removed 8 days after tumor onset were increased 2.8- and 3.6-fold, respectively, in neonatally sympathectomized mice. Administration of NGF prevented the increase in tumor NE and DA concentration associated with chemical sympathectomy. Cell surface receptors for NGF were present on cultured C-1300 MNB cells. These data suggested the presence of two types of binding sites with K(D) values of 45.9 pM (high affinity) and 2.4 nM (low affinity). The inhibition of MNB tumor growth and elevation in tumor catecholamine content produced by neonatal sympathectomy may be humorally mediated since they were selectively reversed by the administration of NGF. C1 CHILDRENS MEM HOSP,CHILDRENS MEM INST EDUC & RES,BOX 117,2300 CHILDRENS PLAZA,CHICAGO,IL 60614. NICHHD,NIGMS,GROWTH FACTORS SECT,BETHESDA,MD. NR 33 TC 4 Z9 4 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0360-4012 J9 J NEUROSCI RES JI J. Neurosci. Res. PD MAR PY 1991 VL 28 IS 3 BP 343 EP 351 DI 10.1002/jnr.490280305 PG 9 WC Neurosciences SC Neurosciences & Neurology GA FE961 UT WOS:A1991FE96100004 PM 1906944 ER PT J AU ATKINSON, JC FRANK, MM AF ATKINSON, JC FRANK, MM TI ORAL MANIFESTATIONS AND DENTAL MANAGEMENT OF PATIENTS WITH HEREDITARY ANGIOEDEMA SO JOURNAL OF ORAL PATHOLOGY & MEDICINE LA English DT Article DE ANGIOEDEMA, HEREDITARY; COMPLEMENT; DENTAL TREATMENT ID C1 ESTERASE INHIBITOR; ANGIONEUROTIC-EDEMA; DEFICIENCY; DANAZOL; SURGERY AB Hereditary angioedema (HAE) is a genetic disorder in which affected individuals develop extensive, spontaneous angioedema of the extremities, gastrointestinal tract, and oropharynx. Dental treatment of unmedicated patients with HAE can trigger life-threatining pharyngeal edema. Previously, it was demonstrated that the administration of fresh frozen plasma (FFP) before surgery prevented angioedma attacks in 6 patients undergoing dental extractions. The present study examines the long term effectiveness of FFP in preventing angioedema from developing in 53 patients with HAE undergoing all types of dental treatment over a ten-year period. Only 3 of 45 patients (6.7%) covered with FFP had a minor angioedema attack after dental therapy in 10 yr. No attacks of moderate or severe swelling were seen. Attacks occurred independently of the disease activity of the patient and the trauma of the dental procedure. The use of fresh frozen plasma is effective in preventing attacks of angioedema in HAE patients undergoing all types of dental procedures. C1 NIAID,CLIN INVEST LAB,BETHESDA,MD 20892. NIDR,PATIENT CARE BRANCH,BETHESDA,MD 20892. RP ATKINSON, JC (reprint author), NIDR,CLIN INVEST BRANCH,BLDG 10,ROOM 1N-113,BETHESDA,MD 20892, USA. NR 17 TC 26 Z9 26 U1 0 U2 1 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0904-2512 J9 J ORAL PATHOL MED JI J. Oral Pathol. Med. PD MAR PY 1991 VL 20 IS 3 BP 139 EP 142 DI 10.1111/j.1600-0714.1991.tb00908.x PG 4 WC Dentistry, Oral Surgery & Medicine; Pathology SC Dentistry, Oral Surgery & Medicine; Pathology GA FF606 UT WOS:A1991FF60600009 PM 1828083 ER PT J AU HANNA, JD CHAN, JCM GILL, JR AF HANNA, JD CHAN, JCM GILL, JR TI HYPERTENSION AND THE KIDNEY SO JOURNAL OF PEDIATRICS LA English DT Article ID BLOOD-PRESSURE; WEIGHT-LOSS; NEONATAL HYPERTENSION; CALCIUM-METABOLISM; OBESE ADOLESCENTS; AEROBIC EXERCISE; FOLLOW-UP; SODIUM; SALT; CAPTOPRIL C1 VIRGINIA COMMONWEALTH UNIV, MED COLL VIRGINIA,DIV HLTH SCI,BOX 498, MED COLL VIRGINIA STN, RICHMOND, VA 23298 USA. NHLBI, HYPERTENS ENDOCRINE BRANCH, BETHESDA, MD 20892 USA. FU NIDDK NIH HHS [T32 DK 07526] NR 57 TC 16 Z9 16 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-3476 EI 1097-6833 J9 J PEDIATR-US JI J. Pediatr. PD MAR PY 1991 VL 118 IS 3 BP 327 EP 340 DI 10.1016/S0022-3476(05)82143-2 PG 14 WC Pediatrics SC Pediatrics GA FB292 UT WOS:A1991FB29200001 PM 1999772 ER PT J AU SHARPE, LG AF SHARPE, LG TI SEPARATE NEURAL MECHANISMS MEDIATE SUFENTANIL-INDUCED PUPILLARY RESPONSES IN THE CAT SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID MORPHINE-INDUCED MYDRIASIS; EDINGER-WESTPHAL NUCLEUS; BINDING-SITES; MU-OPIATE; RAT; CLONIDINE; DOG; FLUCTUATION; NALOXONE; OPIOIDS AB The pharmacologic characteristics of a highly selective mu receptor agonist, sufentanil, were studied on the cat's pupillary responses (size, light reflex and fluctuations) measured with an infrared video pupillometer. The pupillary effects of sufentanil were also compared with those of morphine and clonidine, known mydriatics in the cat. Sufentanil (0.3-10-mu-g/kg i.v.) dose-dependently increased pupillary size and decreased light reflex and fluctuations. Naltrexone (10-mu-g/kg i.v.) pretreatment shifted the dose-response curve to the right by a factor of 26 for pupillary size, 9.5 for light reflex and 7.2 for fluctuations (nonvalid bioassay). Equivalent mydriatic doses of sufentanil (1-mu-g/kg), morphine (0.5 mg/kg) and clonidine (10-mu-g/kg) produced divergent effects on the light reflex and fluctuations. At these doses, morphine was more effective than sufentanil in inhibiting fluctuations. Clonidine was a more potent inhibitor of fluctuations but significantly enhanced the light reflex. Sufentanil (compared with morphine in a previous study) was 298 times more potent than morphine as a mydriatic, 100 times more potent in inhibiting the light reflex, and only slightly more potent in inhibiting fluctuations. These results indicate that separate neural mechanisms control the three pupillary components and that mu opioid receptors are more involved in mediating opiate-induced mydriasis than in inhibiting the light reflex and fluctuations in the cat. RP SHARPE, LG (reprint author), NIDA,ADDICT RES CTR,POB 5180,BALTIMORE,MD 21224, USA. NR 28 TC 5 Z9 6 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD MAR PY 1991 VL 256 IS 3 BP 845 EP 849 PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FC923 UT WOS:A1991FC92300005 PM 1672378 ER PT J AU ELMER, GI GEORGE, FR AF ELMER, GI GEORGE, FR TI THE ROLE OF PROSTAGLANDIN SYNTHETASE IN THE RATE DEPRESSANT EFFECTS AND NARCOSIS CAUSED BY ETHANOL SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID ANTI-INFLAMMATORY DRUGS; BRED MOUSE LINES; BRAIN MEMBRANES; ARACHIDONIC-ACID; ANTIINFLAMMATORY AGENTS; ALCOHOL-INTOXICATION; PHYSICAL-PROPERTIES; ENDOTHELIAL-CELLS; MOTOR IMPAIRMENT; PHASE-TRANSITION AB Ethanol is proposed to exert its pharmacological effects by increasing membrane fluidity. Support for this hypothesis comes from strong correlations between in vitro effects of ethanol and pharmacological effects and genetic variation seen in vivo. Because arachidonate acid (AA) cascade is a membrane-bound system activated by disruptions in the cell membrane, it is possible that ethanol-induced membrane fluidization increases the formation of AA metabolites such as prostaglandins. The studies reported here were designed to characterize systematically the ability of various prostaglandin endoperoxide synthetase (PES; prostaglandin synthetase or cyclooxygenase) inhibitors to antagonize the effects of ethanol. A wide range of chemically diverse PES inhibitors significantly antagonized the rate-depressant effects and acute narcosis caused by ethanol. The ability of these compounds to antagonize the effects of ethanol was significantly correlated with two measures of PES activity; in vitro inhibition of the PES enzyme and in vivo anti-inflammatory activity. These significant potency correlations between the ability of PES inhibitors to antagonize in vivo acute ethanol hypnosis and their ability to inhibit in vitro and in vivo conversion of AA to AA metabolites strongly suggest an underlying functional relationship. These results obtained from two divergent dependent measures of the effects of ethanol, duration of loss of the righting reflex and rate depressant effects, extend the range of ethanol-related behaviors that PES inhibitors effectively antagonize and provide substantive evidence in support of a mechanistic role for PES and related metabolites in the central effects of ethanol. C1 UNIV MARYLAND, SCH PHARM, DEPT PHARMACOL & TOXICOL, BALTIMORE, MD 21201 USA. RP NIDA, ADDICT RES CTR, PRECLIN PHARMACOL BRANCH, BEHAV PHARMACOL & GENET LAB, 4940 EASTERN AVE, BALTIMORE, MD 21201 USA. FU NIAAA NIH HHS [AA-06104/06924, AA-07754] NR 88 TC 12 Z9 13 U1 0 U2 0 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 0022-3565 EI 1521-0103 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD MAR PY 1991 VL 256 IS 3 BP 1139 EP 1146 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FC923 UT WOS:A1991FC92300046 PM 1900890 ER PT J AU TOKUNAGA, M LAND, CE TOKUOKA, S AF TOKUNAGA, M LAND, CE TOKUOKA, S TI FOLLOW-UP-STUDIES OF BREAST-CANCER INCIDENCE AMONG ATOMIC-BOMB SURVIVORS SO JOURNAL OF RADIATION RESEARCH LA English DT Article DE BREAST CANCER; ATOMIC BOMBING; RADIATION; CARCINOGENESIS; EPIDEMIOLOGY ID ACUTE POSTPARTUM MASTITIS; IONIZING-RADIATION; WOMEN; HIROSHIMA; NAGASAKI; RISK; INFANCY; CHEST C1 KAGOSHIMA MUNICIPAL HOSP,DEPT PATHOL,KAGOSHIMA 892,JAPAN. NCI,RADIAT EPIDEMIOL BRANCH,BETHESDA,MD 20892. RP TOKUNAGA, M (reprint author), RADIAT EFFECTS RES FDN,DEPT EPIDEMIOL PATHOL,HIROSHIMA 730,JAPAN. NR 31 TC 14 Z9 14 U1 0 U2 0 PU JAPAN RADIATION RESEARCH SOC PI CHIBA PA C/O NAT INST RADIOLOGICAL SCI 9-1 ANAGAWA-4-CHOME INAGE-KU, CHIBA 263, JAPAN SN 0449-3060 J9 J RADIAT RES JI J. Radiat. Res. PD MAR PY 1991 VL 32 SU S BP 201 EP 211 DI 10.1269/jrr.32.SUPPLEMENT_201 PG 11 WC Biology; Radiology, Nuclear Medicine & Medical Imaging SC Life Sciences & Biomedicine - Other Topics; Radiology, Nuclear Medicine & Medical Imaging GA GE293 UT WOS:A1991GE29300025 PM 1762108 ER PT J AU PILLEMER, SR AF PILLEMER, SR TI FLOW-CYTOMETRY AND THE RHEUMATIC DISEASES - REPLY SO JOURNAL OF RHEUMATOLOGY LA English DT Letter RP PILLEMER, SR (reprint author), NIAMS,OPECA,BETHESDA,MD 20892, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD MAR PY 1991 VL 18 IS 3 BP 478 EP 478 PG 1 WC Rheumatology SC Rheumatology GA FE552 UT WOS:A1991FE55200037 ER PT J AU FOLLMANN, DA LAMBERT, D AF FOLLMANN, DA LAMBERT, D TI IDENTIFIABILITY OF FINITE MIXTURES OF LOGISTIC-REGRESSION MODELS SO JOURNAL OF STATISTICAL PLANNING AND INFERENCE LA English DT Article DE LOGIT MIXTURE; RANDOM EFFECT AB We allow the intercept in logistic regression to have a nonparametric, discrete distribution and give simple conditions that ensure that the vector of fixed regression coefficients and the mixing distribution of the intercept are identifiable. For binary responses, the number of atoms in the mixing distribution must be bounded by a function of the number of covariate vectors that agree except for one coordinate. For binomial responses, the number of atoms must satisfy the same bound or be bounded by a function of the largest number of trials per response. C1 NHLBI,BETHESDA,MD 20892. AT&T BELL LABS,MURRAY HILL,NJ 07974. NR 13 TC 31 Z9 31 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3758 J9 J STAT PLAN INFER JI J. Stat. Plan. Infer. PD MAR PY 1991 VL 27 IS 3 BP 375 EP 381 DI 10.1016/0378-3758(91)90050-O PG 7 WC Statistics & Probability SC Mathematics GA FB671 UT WOS:A1991FB67100009 ER PT J AU HIRAMATSU, R NISULA, BC AF HIRAMATSU, R NISULA, BC TI UPTAKE OF ERYTHROCYTE-ASSOCIATED COMPONENT OF BLOOD TESTOSTERONE AND CORTICOSTERONE TO RAT-BRAIN SO JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article ID TRANSPORT; CORTISOL; HORMONES; BARRIER AB To study transport of steroids by erythrocytes, the tissue uptake of erthyrocyte-associated testosterone and corticosterone was studied in vivo using a single injection technique into the carotid artery of rats. A brain uptake index (BUI) was calculated by dividing the ratio of [H-3]steroid to [C-14]butanol (internal reference) in the brain tissue by that in the injection material, and multiplying by 100%. BUIs of testosterone and corticosterone in an erythrocyte suspension were 131 +/- 3% (mean +/- SE, n = 6) and 57.0 +/- 2.7% (n = 6), respectively, which were greater than those in buffer (100 +/- 4%; n = 4, P < 0.01 and 39.8 +/- 4.6%; n = 4, P < 0.01, respectively). The erythrocyte accounted for 83.9% and 76.7% of the total testosterone and corticosterone delivered to the tissues, respectively, when calculated on the assumption that the BUIs of steroid in buffer and in the supernatant of an erythrocyte suspension are the same. BUIs of corticosterone in hemolysate and in a suspension of erythrocyte plasma membranes (60.8 +/- 7.0%; n = 4 and 69.5 +/- 3.7%; n = 4, respectively) were also greater than those in buffer (P < 0.05 and P < 0.01, respectively). Our results suggest that the erythrocyte-associated component of testosterone and corticosterone are delivered to the tissue of rat brain, and that their membranes may play a major role in their capacity to transport steroids to the tissues. C1 KUMAMOTO UNIV,SCH MED,DEPT INTERNAL MED 3,KUMAMOTO 860,JAPAN. NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. NR 13 TC 4 Z9 4 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0960-0760 J9 J STEROID BIOCHEM JI J. Steroid Biochem. Mol. Biol. PD MAR PY 1991 VL 38 IS 3 BP 383 EP 387 DI 10.1016/0960-0760(91)90111-H PG 5 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA FG558 UT WOS:A1991FG55800014 PM 2009229 ER PT J AU GORDIS, E AF GORDIS, E TI FROM SCIENCE TO SOCIAL-POLICY - AN UNCERTAIN ROAD SO JOURNAL OF STUDIES ON ALCOHOL LA English DT Article ID TAXES; DRINKING RP GORDIS, E (reprint author), NIAAA,ROCKVILLE,MD 20857, USA. NR 10 TC 15 Z9 15 U1 0 U2 1 PU ALCOHOL RES DOCUMENTATION INC CENT ALCOHOL STUD RUTGERS UNIV PI PISCATAWAY PA PO BOX 969, PISCATAWAY, NJ 08855-0969 SN 0096-882X J9 J STUD ALCOHOL JI J. Stud. Alcohol PD MAR PY 1991 VL 52 IS 2 BP 101 EP 109 PG 9 WC Substance Abuse; Psychology SC Substance Abuse; Psychology GA EY615 UT WOS:A1991EY61500001 PM 2016869 ER PT J AU JENSEN, PS RICHTERS, J USSERY, T BLOEDAU, L DAVIS, H AF JENSEN, PS RICHTERS, J USSERY, T BLOEDAU, L DAVIS, H TI CHILD PSYCHOPATHOLOGY AND ENVIRONMENTAL-INFLUENCES - DISCRETE LIFE EVENTS VERSUS ONGOING ADVERSITY SO JOURNAL OF THE AMERICAN ACADEMY OF CHILD AND ADOLESCENT PSYCHIATRY LA English DT Article DE STRESS; PARENTAL PSYCHOPATHOLOGY; CHILD PSYCHOPATHOLOGY; CHILDRENS SYMPTOMATOLOGY ID ETIOLOGIC FACTORS; STRESS; ADOLESCENCE; DEPRESSION; DISORDERS; DISEASES; BEHAVIOR AB Patterns of exposure to distinct types of life stressors were compared between 134-children attending a military child psychiatric clinic and a matched military community control sample. Compared with the community sample, clinic-referred children had experienced significantly higher levels of normative stressful events as well as events confounded with their own adjustment and events related to parental psychosocial functioning. Differences in levels of normative stressful events were no longer significant, however, when controlling for events related to parental functioning. Ratings of stressful events during the past year significantly underestimated the lifetime stress exposure differences between clinic and community control children. Although normative stressful events, parent-related events, and parent symptomatology ratings were significantly related to child behavior problem ratings, normative stressful events did not contribute to predictions of child behavior problems beyond the variance attributable to parent-related events and parent symptomatology. Implications of these findings for life stress and child maladjustment research are discussed. C1 WALTER REED ARMY MED CTR,DEPT MILITARY PSYCHIAT,WASHINGTON,DC 20307. MED COLL GEORGIA,AUGUSTA,GA 30912. RP JENSEN, PS (reprint author), NIMH,CHILD & ADOLESCENT DISORDERS RES BRANCH,ROOM 10-104,ROCKVILLE,MD 20857, USA. OI Richters, John/0000-0002-6780-1828; Jensen, Peter/0000-0003-2387-0650 NR 42 TC 36 Z9 36 U1 0 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0890-8567 J9 J AM ACAD CHILD PSY JI J. Am. Acad. Child Adolesc. Psychiatr. PD MAR PY 1991 VL 30 IS 2 BP 303 EP 309 DI 10.1097/00004583-199103000-00021 PG 7 WC Psychology, Developmental; Pediatrics; Psychiatry SC Psychology; Pediatrics; Psychiatry GA FB921 UT WOS:A1991FB92100021 PM 2016236 ER PT J AU GREENBERG, JD KRUESI, MJP KEYSOR, CS GROTHE, DR AF GREENBERG, JD KRUESI, MJP KEYSOR, CS GROTHE, DR TI PRICES OF PSYCHOTROPIC MEDICATIONS USED IN THE TREATMENT OF PSYCHIATRIC-ILLNESS IN CHILDREN AND ADOLESCENTS SO JOURNAL OF THE AMERICAN ACADEMY OF CHILD AND ADOLESCENT PSYCHIATRY LA English DT Note C1 NIMH,CHILD PSYCHIAT BRANCH,BLDG 10,ROOM 6N-240,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NIMH,DEPT CLIN CTR PHARM,BETHESDA,MD 20892. NR 6 TC 1 Z9 1 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0890-8567 J9 J AM ACAD CHILD PSY JI J. Am. Acad. Child Adolesc. Psychiatr. PD MAR PY 1991 VL 30 IS 2 BP 323 EP 324 DI 10.1097/00004583-199103000-00024 PG 2 WC Psychology, Developmental; Pediatrics; Psychiatry SC Psychology; Pediatrics; Psychiatry GA FB921 UT WOS:A1991FB92100024 PM 2016239 ER PT J AU GOLDBERGER, E RAPOPORT, JL AF GOLDBERGER, E RAPOPORT, JL TI CANINE ACRAL LICK DERMATITIS - RESPONSE TO THE ANTIOBSESSIONAL DRUG CLOMIPRAMINE SO JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION LA English DT Article ID OBSESSIVE-COMPULSIVE DISORDER; DESIPRAMINE; DOGS AB Treatment of canine acral lick dermatitis (ALD) with two tricyclic antidepressant drugs was examined in a single-blind comparison of clomipramine, a new serotonin reuptake blocking tricyclic, and desipramine, a standard tricyclic antidepressant. Six of nine dogs showed an initial response to clomipramine and completed all three five-week phases of the A-B-A design study, taking clomipramine first, then desipramine, then clomipramine again. The owners were blind to drug identity and rated their dogs' licking behavior on weekly basis. Treatment with clomipramine but not desipramine resulted in significant improvement in the dogs' licking behavior. Results of this study suggest that clomipramine may be an effective treatment for ALD. In addition, the selective success of clomipramine in treatment of ALD is strikingly similar to the selective success of this drug in the treatment of obsessive compulsive disorder, suggesting a link between the abnormal "grooming behavior" in dogs and the abnormal psychiatric behavior in humans with obsessive compulsive disorder. C1 NIMH,CHILD PSYCHIAT BRANCH,BLDG 10,ROOM 6N240,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 17 TC 63 Z9 63 U1 1 U2 1 PU AMER ANIMAL HOSPITAL ASSOC PI LAKEWOOD PA PO BOX 150899, LAKEWOOD, CO 80215-0899 SN 0587-2871 J9 J AM ANIM HOSP ASSOC JI J. Am. Anim. Hosp. Assoc. PD MAR-APR PY 1991 VL 27 IS 2 BP 179 EP 182 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA FB886 UT WOS:A1991FB88600006 ER PT J AU PANZA, JA QUYYUMI, AA DIODATI, JG CALLAHAN, TS EPSTEIN, SE AF PANZA, JA QUYYUMI, AA DIODATI, JG CALLAHAN, TS EPSTEIN, SE TI PREDICTION OF THE FREQUENCY AND DURATION OF AMBULATORY MYOCARDIAL-ISCHEMIA IN PATIENTS WITH STABLE CORONARY-ARTERY DISEASE BY DETERMINATION OF THE ISCHEMIC THRESHOLD FROM EXERCISE TESTING - IMPORTANCE OF THE EXERCISE PROTOCOL SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID HEART-DISEASE; ANGINA-PECTORIS; SILENT; SEVERITY; STRESS AB The relation between ambulatory myocardial ischemia and the results of exercise testing in patients with ischemic heart disease remains undefined, because of the dissimilar results of previous reports. To further investigate this issue and, in particular, to ascertain the importance of the exercise protocol in determining that relation, 70 patients with stable coronary artery disease underwent 48 h ambulatory electrocardiographic (ECG) monitoring and treadmill exercise tests after withdrawal of medications. Patients exercised using two different protocols with slow (National Institutes of Health [NIH] combined protocol) and brisk (Bruce protocol) work load increments. Exercise duration was longer with the NIH combined protocol (14.1 +/- 5 versus 6.8 +/- 2 min; p < 0.0001), but the maximal work load and peak heart rate achieved were greater with the Bruce protocol (9.8 +/- 2 versus 6.5 +/- 2 METs, and 142 +/- 19 versus 133 +/- 22 beats/min, respectively; p < 0.0001). A close inverse correlation between exercise testing and the results of ambulatory ECG monitoring was observed using the NIH combined protocol; the strongest correlation was observed between time of exercise at 1 mm of ST segment depression and number of ischemic episodes (r = -0.86; p < 0.0001). With the Bruce protocol a significantly weaker inverse correlation was found (r = -0.35). The mean heart rate at the onset of ST segment depression was similar during monitoring and during exercise testing with the NIH combined protocol (97.2 +/- 13 versus 101.0 +/- 17 beats/min, respectively) but it was significantly higher (110.4 +/- 13) when using the Bruce protocol (p < 0.001). These findings indicate that a relation does exist between ambulatory myocardial ischemia and the results of the exercise test but this relation is critically determined by the exercise protocol and is better observed with protocols that produce slow work load increments. These observations raise questions about the independent value and usefulness of ambulatory ECG monitoring in patients with stable ischemic heart disease. RP PANZA, JA (reprint author), NHLBI,CARDIOL BRANCH,BLDG 10,ROOM 7B-15,BETHESDA,MD 20892, USA. NR 19 TC 49 Z9 49 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD MAR 1 PY 1991 VL 17 IS 3 BP 657 EP 663 PG 7 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA EY741 UT WOS:A1991EY74100012 PM 1993784 ER PT J AU ROBERTS, CS ROBERTS, WC AF ROBERTS, CS ROBERTS, WC TI DISSECTION OF THE AORTA ASSOCIATED WITH CONGENITAL-MALFORMATION OF THE AORTIC-VALVE SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID ANEURYSM; NECROPSY; STENOSIS AB The association of congenital aortic valve malformation and aortic dissection is analyzed. Over a 30 year period, 186 patients with noniatrogenic aortic dissection were studied at necropsy. The aortic valve was tricuspid in 170 (91.4%), bicuspid in 14 (7.5%) and unicuspid in 2 (1.1%). Among the 16 patients with aortic dissection and a congenitally malformed valve, the age at death ranged from 17 to 82 years (mean 52) and 13 (81%) were men. The entrance tear of the aortic dissection was located in the ascending aorta in all 16 patients with a malformed valve but in only 68% of those with a tricuspid aortic valve. The aortic valve was stenotic in 6 of the 16 patients with a congenitally malformed valve. Fatal rupture of the false channel occurred after acute ascending aortic dissection in each of the 11 patients (none with healed dissection) who did not have operative therapy for the dissection. Two of the 16 patient with a malformed valve compared with no patient with a tricuspid aortic valve had aortic isthmic coarctation. Histologic sections of aorta from 10 patients disclosed severe degeneration of the elastic fibers of the media in 9 patients. Thus, a congenitally malformed aortic valve appears to be present at least 5 times more frequently in adults with than in those without aortic dissection, and in our patients the entrance tear was always in the ascending aorta, which usually had severe loss of elastic fibers in its media. C1 NHLBI,PATHOL BRANCH,BLDG 10,ROOM 2N258,BETHESDA,MD 20892. NHLBI,SURG BRANCH,BETHESDA,MD 20892. NR 14 TC 151 Z9 153 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD MAR 1 PY 1991 VL 17 IS 3 BP 712 EP 716 PG 5 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA EY741 UT WOS:A1991EY74100020 PM 1993792 ER PT J AU MANOLIO, TA LEVY, D GARRISON, RJ CASTELLI, WP KANNEL, WB AF MANOLIO, TA LEVY, D GARRISON, RJ CASTELLI, WP KANNEL, WB TI RELATION OF ALCOHOL INTAKE TO LEFT-VENTRICULAR MASS - THE FRAMINGHAM-STUDY SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID CORONARY HEART-DISEASE; ETHANOL; MUSCLE; CARDIOMYOPATHY; ABNORMALITIES; HYPERTENSION; CONSUMPTION; MORTALITY; SKELETAL; AGE AB Alcohol has direct toxic effects on the myocardium and is associated with elevated blood pressure, but its relation to left ventricular mass independent of blood pressure level has not been assessed. Reported alcohol intake and left ventricular mass measured by echocardiography were evaluated in 1,980 men and 2,511 women 17 to 90 years of age and free of cardiovascular disease in the Framingham offspring and cohort study. The relation of reported alcohol intake to left ventricular mass was assessed by gender-specific multivariate regression analysis adjusting for age, height, body mass index, systolic blood pressure, history of hypertension and cigarette smoking. Alcohol intake was positively associated with left ventricular mass in men (p < 0.01) but not in women (p = 0.64). When stratified by beverage type, beer and wine in both men and women and liquor in men were positively related to left ventricular mass. The lack of association of total alcohol intake to left ventricular mass in women appeared to be due to a negative association (p < 0.01) with liquor. The strongest positive associations were with wine in men (p < 0.001) and beer in women (p < 0.05). Alcohol use is independently associated with left ventricular mass; this association may vary by beverage type. In persons with unexplained left ventricular hypertrophy, excessive alcohol intake should be considered. C1 NHLBI,FRAMINGHAM HEART STUDY,FRAMINGHAM,MA. BOSTON UNIV,SCH MED,PREVENT MED & EPIDEMIOL SECT,BOSTON,MA 02118. RP MANOLIO, TA (reprint author), NHLBI,DIV EPIDEMIOL & CLIN APPLICAT,EBP,CLIN & GENET EPIDEMIOL BRANCH,BETHESDA,MD 20892, USA. FU NHLBI NIH HHS [HC-38038] NR 28 TC 89 Z9 90 U1 2 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD MAR 1 PY 1991 VL 17 IS 3 BP 717 EP 721 PG 5 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA EY741 UT WOS:A1991EY74100021 PM 1825213 ER PT J AU FLEG, JL ROTHFELD, B GOTTLIEB, SH WRIGHT, J AF FLEG, JL ROTHFELD, B GOTTLIEB, SH WRIGHT, J TI EFFECT OF MAINTENANCE DIGOXIN THERAPY ON AEROBIC PERFORMANCE AND EXERCISE LEFT-VENTRICULAR FUNCTION IN MILD TO MODERATE HEART-FAILURE DUE TO CORONARY-ARTERY DISEASE - A RANDOMIZED, PLACEBO-CONTROLLED, CROSSOVER TRIAL SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID SINUS RHYTHM; CAPACITY; REST AB Despite 200 years of use, the ability of digitalis glycosides to improve exercise capacity in patients with congestive heart failure remains controversial, partly because of imprecise end points and suboptimal study design. Therefore, this question was examined in 10 ambulatory patients (8 men and 2 women) aged 46 to 70 years (mean 57.8) in sinus rhythm with mild to moderate chronic stable congestive heart failure due to coronary artery disease and systolic left ventricular dysfunction (ejection fraction 32 +/- 12). All underwent maximal treadmill exercise with respiratory gas analysis and upright cycle ergometry with gated radionuclide angiography after 4 weeks of digoxin or placebo therapy, administered in a randomized double-blind crossover protocol. Neither treadmill exercise duration (7.7 +/- 2.3 versus 7.3 +/- 2.7 min) nor peak oxygen consumption (18.7 +/- 3.7 versus 18.4 +/- 5.4 ml/kg per min) differed between digoxin and placebo regimens. However, the change in peak oxygen consumption induced by digoxin was inversely related to the peak oxygen consumption during placebo therapy (r = -0.64, p < 0.05). At maximal treadmill effort, heart rate (138 +/- 16 versus 141 +/- 21 beats/min), oxygen pulse (10.3 +/- 2.1 versus 9.9 +/- 2.2 ml/beat), ventilation (40.3 +/- 10.6 versus 42.0 +/- 10.8 liters/min) and ventilatory equivalent (29.4 +/- 4.8 versus 31.5 +/- 6.8) did not differ between digoxin and placebo treatment, although systolic blood pressure was higher during digoxin therapy (163.0 +/-23.1 versus 153.2 +/- 25.3 mm Hg, p < 0.05). Left ventricular ejection fraction at rest (32 +/- 12 versus 29 +/- 12) and maximal cycle work load achieved (75 +/- 32 versus 75 +/- 28 W) were not affected by glycoside treatment, but ejection fraction and systolic blood pressure at maximal effort were both higher during digoxin than during placebo therapy (32 +/- 8 versus 27 +/- 9, p < 0.05, and 177 +/- 25 versus 165 +/- 21 mm Hg, p < 0.05, respectively). The change in peak treadmill oxygen consumption and the change in peak ergometric left ventricular ejection fraction induced by digoxin were not related (r = -.035, p = NS). Therefore, in ambulatory patients with coronary artery disease and chronic, predominantly mild to moderate heart failure, systolic left ventricular dysfunction and sinus rhythm, maintenance digoxin treatment did not elicit an increase in aerobic capacity despite improvement in left ventricular performance during exercise. Nevertheless, the inverse relation between the peak oxygen consumption during placebo administration and the change in this variable elicited by digoxin suggest that exercise testing with respiratory gas analysis may provide a rational basis for identifying ambulatory patients with heart failure who are likely to benefit from maintenance digoxin therapy. C1 FRANCIS SCOTT KEY MED CTR,DIV CARDIOL,BALTIMORE,MD. FRANCIS SCOTT KEY MED CTR,DIV NUCL MED,BALTIMORE,MD. RP FLEG, JL (reprint author), NIA,GERONTOL RES CTR,CARDIOVASC SCI LAB,4940 EASTERN AVE,BETHESDA,MD 20892, USA. NR 26 TC 43 Z9 43 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD MAR 1 PY 1991 VL 17 IS 3 BP 743 EP 751 PG 9 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA EY741 UT WOS:A1991EY74100026 PM 1993796 ER PT J AU SHIP, JA FOX, PC BAUM, BJ AF SHIP, JA FOX, PC BAUM, BJ TI HOW MUCH SALIVA IS ENOUGH - NORMAL FUNCTION DEFINED SO JOURNAL OF THE AMERICAN DENTAL ASSOCIATION LA English DT Article ID DIFFERENT AGE-GROUPS; FLOW-RATE; GLAND HYPOFUNCTION; DRY MOUTH; XEROSTOMIA; SIALOCHEMISTRY; DIAGNOSIS; SYMPTOM; WHOLE; PH AB Saliva is important for the preservation and maintenance of oral health. It is unclear, however, how much saliva is required to maintain normal oral function. Major salivary gland flow rates, objective measurements of oral health, and subjective complaints of oral problems were assessed in different-aged, healthy persons. Results suggest that the comparison of major salivary gland flow rates of an individual with population standards to identify patients susceptible to the effects of salivary dysfunction is unreliable. Changes in salivary function over time are a more meaningful gauge of the impact of saliva on oral health. The clinician should monitor salivary production to identify patients with declining salivary gland output. C1 NIDR,CLIN INVEST & PATIENT CARE BRANCH,CLIN INVEST SECT,BETHESDA,MD 20892. RP SHIP, JA (reprint author), NIDR,CLIN INVEST & PATIENT CARE BRANCH,9000 ROCKVILLE PIKE,BLDG 10,BETHESDA,MD 20892, USA. NR 35 TC 158 Z9 160 U1 3 U2 14 PU AMER DENTAL ASSN PI CHICAGO PA 211 E CHICAGO AVE, CHICAGO, IL 60611 SN 0002-8177 J9 J AM DENT ASSOC JI J. Am. Dent. Assoc. PD MAR PY 1991 VL 122 IS 3 BP 63 EP 69 PG 7 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA FB477 UT WOS:A1991FB47700016 PM 2019691 ER PT J AU SHETTY, HU DALY, EM GREIG, NH RAPOPORT, SI SONCRANT, TT AF SHETTY, HU DALY, EM GREIG, NH RAPOPORT, SI SONCRANT, TT TI AN AUTOMATIC REACTION CONTROL CHEMICAL IONIZATION TECHNIQUE IN ION TRAP DETECTOR FOR QUANTITATIVE PLASMA PROFILING OF ARECOLINE IN TREATED ALZHEIMER PATIENTS SO JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY LA English DT Article ID SENILE DEMENTIA; MASS-SPECTROMETRY; DISEASE; NEURONS; CHOLINE; TISSUE AB An automatic reaction control chemical ionization technique in an ion trap detector (ITD) was used to quantitate the levels of the cholinergic drug, arecoline, in plasma of treated patients with Alzheimer's disease. The chemical ionization reaction was carried out with acetonitrile. The protonated molecules of arecoline (m/z 156) and the internal standard, homoarecoline (m/z 170), were monitored. Human plasma samples were extracted with a readily evaporable solvent mixture, the residues reconstituted and injected along with a tertiary amine-carrier into a capillary gas chromatograph interfaced with the ITD. Standard curves for plasma-extracted arecoline between 20-ng/mL and 156-pg/mL levels were linear (r > 0.9980). Satisfactory precision (relative standard deviation < 20%) and accuracy (relative error < 15%) at the limit of quantitation, 156 pg/mL arecoline, were achieved. Optimal conditions for handling of blood samples obtained by venipuncture were determined. The assay was successfully applied for the therapeutic monitoring of Alzheimer patients treated intravenously with arecoline. C1 NIA,NEUROSCI LAB,BETHESDA,MD. NR 22 TC 17 Z9 17 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 1044-0305 J9 J AM SOC MASS SPECTR JI J. Am. Soc. Mass Spectrom. PD MAR-APR PY 1991 VL 2 IS 2 BP 168 EP 173 DI 10.1016/1044-0305(91)80011-U PG 6 WC Chemistry, Analytical; Chemistry, Physical; Spectroscopy SC Chemistry; Spectroscopy GA FC743 UT WOS:A1991FC74300011 PM 24242177 ER PT J AU LEVIN, NW STRIKER, GE AF LEVIN, NW STRIKER, GE TI RESEARCH ON PROBLEMS OF THE DIALYSIS PATIENT SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Editorial Material C1 BETH ISRAEL MED CTR,NEW YORK,NY 10003. NIH,BETHESDA,MD 20892. NR 0 TC 8 Z9 8 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD MAR PY 1991 VL 1 IS 9 BP 1055 EP 1056 PG 2 WC Urology & Nephrology SC Urology & Nephrology GA FT107 UT WOS:A1991FT10700001 PM 1912404 ER PT J AU LEVEY, AS GASSMAN, JJ HALL, PM WALKER, WG AF LEVEY, AS GASSMAN, JJ HALL, PM WALKER, WG TI ASSESSING THE PROGRESSION OF RENAL-DISEASE IN CLINICAL-STUDIES - EFFECTS OF DURATION OF FOLLOW-UP AND REGRESSION TO THE MEAN SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Article DE RENAL FUNCTION MEASUREMENT; CHRONIC RENAL DISEASE; DIETARY MODIFICATION; CLINICAL TRIALS ID GLOMERULAR-FILTRATION-RATE; SINGLE SUBCUTANEOUS INJECTION; LOW-NITROGEN DIET; DIABETIC NEPHROPATHY; AMINO-ACIDS; FAILURE; PROTEIN; RESTRICTION AB Many clinical studies of the effects of low-protein and low-phosphorus diets on the course of chronic renal disease have used the rate of decline in renal function to assess the rate of progression. In this report, data from the feasibility phase of the Modification of Diet in Renal Disease Study were used to analyze methods used in other studies. The focus is particularly on the effects of duration of follow-up and of regression to the mean. The findings are summarized as follows. (1) During the mean follow-up period of 14.1 months, rates of decline in glomerular filtration rate, creatinine clearance, and the reciprocal of the serum creatinine concentration were highly variable among individuals, and mean rates of decline were slow. (2) Precision of estimates of individual rates of decline in renal function were relatively low and improved with increasing duration of follow-up. (3) Correlations between rates of decline in creatinine clearance and the reciprocal of the serum creatinine concentration with glomerular filtration rate in individuals were significant but weak and became stronger with increasing duration of follow-up. (4) After entry into the study, mean rate of decline in the reciprocal of the serum creatinine concentration became less negative. The change predicted simply from regression to the mean was 68.4% of the observed change. It was concluded that estimates of the rate of decline in renal function, measured as glomerular filtration rate, creatinine clearance, or the reciprocal of the serum creatinine concentration, may not accurately reflect the true rate of decline during short-term (1- to 2-year) studies in patients with slowly progressive renal disease undergoing dietary modification. In addition, changes in the rate of decline in renal function after entry into studies of dietary modification may be due, in part, to regression to the mean. These results raise questions about the conclusions of previous short-terms studies of the efficacy of dietary modification in retarding the progression of renal disease. Future studies should include a concurrent control group and a longer duration of follow-up and should also include measurements of glomerular filtration rate. C1 NATL INST DIABETES DIGEST & KIDNEY DIS,BETHESDA,MD. NR 33 TC 93 Z9 94 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD MAR PY 1991 VL 1 IS 9 BP 1087 EP 1094 PG 8 WC Urology & Nephrology SC Urology & Nephrology GA FT107 UT WOS:A1991FT10700005 PM 1912407 ER PT J AU STRIKER, GE AF STRIKER, GE TI PROMOTING UROLOGY RESEARCH - WHOSE JOB IS IT ANYWAY SO JOURNAL OF UROLOGY LA English DT Editorial Material RP STRIKER, GE (reprint author), NIDDKD,DIV KIDNEY UROL & HEMATOL DIS,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-5347 J9 J UROLOGY JI J. Urol. PD MAR PY 1991 VL 145 IS 3 BP 566 EP 566 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA FA699 UT WOS:A1991FA69900029 PM 1997713 ER PT J AU BRAREN, V VAUGHAN, ED MOSTOFI, FK PENNINGTON, RD SALVATIERRA, O WEIN, AJ AF BRAREN, V VAUGHAN, ED MOSTOFI, FK PENNINGTON, RD SALVATIERRA, O WEIN, AJ TI LONG-RANGE PLAN OF THE NATIONAL-KIDNEY-AND-UROLOGIC-DISEASES-ADVISORY-BOARD SO JOURNAL OF UROLOGY LA English DT Editorial Material C1 NIDDKD,NATL KIDNEY & UROL DIS ADVISORY BOARD,ROCKVILLE,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-5347 J9 J UROLOGY JI J. Urol. PD MAR PY 1991 VL 145 IS 3 BP 567 EP 567 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA FA699 UT WOS:A1991FA69900030 PM 1997714 ER PT J AU MEN, R BRAY, M LAI, CJ AF MEN, R BRAY, M LAI, CJ TI CARBOXY-TERMINALLY TRUNCATED DENGUE VIRUS ENVELOPE GLYCOPROTEINS EXPRESSED ON THE CELL-SURFACE AND SECRETED EXTRACELLULARLY EXHIBIT INCREASED IMMUNOGENICITY IN MICE SO JOURNAL OF VIROLOGY LA English DT Article ID RECOMBINANT VACCINIA VIRUS; NONSTRUCTURAL PROTEIN NS1; AMINO-ACID SEQUENCE; WEST NILE VIRUS; NEW-GUINEA-C; STRUCTURAL PROTEINS; NUCLEOTIDE-SEQUENCE; ANTIGENIC DETERMINANTS; FLAVIVIRUS; ENCEPHALITIS AB Recombinant vaccinia viruses expressing C-terminally truncated E's that ranged in length from 9 to 99% of the N-terminal sequence were constructed. The overall antigenicity of the E products was analyzed by radioimmunoprecipitation, using dengue virus hyperimmune mouse ascitic fluid (HMAF) or an anti-E peptide serum. Truncated E that was 79% or less in length did not bind HMAF efficiently, whereas E constructs greater than 79% were able to bind HMAF with high efficiency. The first 392 amino acids of the dengue type 4 virus E sequence, including the Arg-392 following the 79% E C terminus, appeared to be critical for proper antigenic structure required for efficient binding by HMAF. Truncated E's ranging from 59 to 81% in length were secreted extracellularly, whereas smaller or larger E's were retained intracellularly. Secreted E's contained carbohydrate side chains that were resistant to endoglycosidase H digestion, suggesting that the transport of E occurs via a pathway from the rough endoplasmic reticulum through the Golgi complex. 79% E-RKG (which possessed the three additional amino acids immediately downstream of 79% E) was expressed at a high concentration on the surface of recombinant virus-infected cells presumably being inserted into the plasma membrane by a hydrophobic C-terminal membrane anchor. Evaluation in mice of the protective efficacy of the various vaccinia virus E recombinants indicated that only truncated E's that were recognized efficiently by HMAF induced a high level of resistance to dengue virus encephalitis. 79% E-RKG which is expressed at a high concentration on the surface of infected cells was highly immunogenic when tested for induction of an E antibody response. This suggests that cell surface expression of 79% E-RKG was responsible for its enhanced immunogenicity. Finally, passive immunization studies indicated that serum antibodies to E played a major role in the complete or nearly complete resistance to dengue virus challenge induced by certain vaccinia virus-truncated E recombinants. C1 NIAID,MOLEC VIRAL BIOL SECT,INFECT DIS LAB,BETHESDA,MD 20892. NR 34 TC 77 Z9 80 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD MAR PY 1991 VL 65 IS 3 BP 1400 EP 1407 PG 8 WC Virology SC Virology GA EY750 UT WOS:A1991EY75000041 PM 1995950 ER PT J AU PARROTT, C SEIDNER, T DUH, E LEONARD, J THEODORE, TS BUCKLERWHITE, A MARTIN, MA RABSON, AB AF PARROTT, C SEIDNER, T DUH, E LEONARD, J THEODORE, TS BUCKLERWHITE, A MARTIN, MA RABSON, AB TI VARIABLE ROLE OF THE LONG TERMINAL REPEAT SP1-BINDING SITES IN HUMAN-IMMUNODEFICIENCY-VIRUS REPLICATION IN LYMPHOCYTE-T SO JOURNAL OF VIROLOGY LA English DT Article ID TUMOR NECROSIS FACTOR; TRANS-ACTIVATOR PROTEIN; GENE-EXPRESSION; SV40 ENHANCER; TRANSCRIPTION FACTOR; FACTOR-ALPHA; NONDEFECTIVE FRIEND; LEUKEMIA-VIRUS; NUCLEAR FACTOR; MESSENGER-RNA AB The long terminal repeat (LTR) of the human immunodeficiency virus (HIV) contains three binding sites for the transcriptional factor Sp1. In order to investigate the role that the Sp1-binding sites play in regulation of HIV replication, we have introduced a deletion of all three Sp1-binding sites into the LTR of an infections molecular clone of HIV. Viral stocks have been prepared from this mutant virus, designated dl-Sp, and these stocks have been used to study its replicative ability in human T cells. The dl-Sp virus replicated efficiently in MT4 cells and in phytohemagglutinin-stimulated human peripheral blood lymphocytes, but it replicated poorly and with delayed kinetics in A3.01 (CEM) T cells unless those cells had been treated with the cytokine tumor necrosis factor alpha. Gel retardation assays to study the levels of DNA-binding proteins present in these cells showed that NF-kappa-B activity could be detected in the nuclei of MT4 cells but not in A3.01 cells unless they had been treated with tumor necrosis factor alpha. Thus, the presence of NF-kappa-B activity appeared to be required for efficient replication of an HIV whose LTR Sp1-binding sites had been deleted. This suggests that NF-kappa-B can functionally compensate for Sp1 in activating HIV replication. The HIV LTR is therefore similar to the promoter-enhancer units of other viruses in that it is composed of multiple functional elements that may contribute differently to viral replication depending on the levels of DNA-binding proteins present in the target cells. C1 NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. HOWARD HUGHES MED INST,BETHESDA,MD 20814. GEORGETOWN UNIV,DIV MOLEC VIROL & IMMUNOL,ROCKVILLE,MD 20852. NR 56 TC 88 Z9 88 U1 1 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD MAR PY 1991 VL 65 IS 3 BP 1414 EP 1419 PG 6 WC Virology SC Virology GA EY750 UT WOS:A1991EY75000043 PM 1995951 ER PT J AU CARPENTER, S ALEXANDERSEN, S LONG, MJ PERRYMAN, S CHESEBRO, B AF CARPENTER, S ALEXANDERSEN, S LONG, MJ PERRYMAN, S CHESEBRO, B TI IDENTIFICATION OF A HYPERVARIABLE REGION IN THE LONG TERMINAL REPEAT OF EQUINE INFECTIOUS-ANEMIA VIRUS SO JOURNAL OF VIROLOGY LA English DT Note ID HUMAN IMMUNODEFICIENCY VIRUS; MINK DISEASE PARVOVIRUS; TUMOR NECROSIS FACTOR; NUCLEOTIDE-SEQUENCE; GENOMIC ORGANIZATION; CONSERVED SEQUENCE; KAPPA-B; GENE; TYPE-1; HOST AB An avirulent, field-derived isolate of equine infectious anemia virus (EIAV), designated MA-1, was molecularly cloned, and the complete nucleotide sequence was determined for the 3' half of the viral genome. Comparisons between MA-1 and the prototype Wyoming strain of EIAV identified a 66-nucleotide stretch between CAAT (-91) and TATAA (-25) in the U3 region of the long terminal repeat, where sequence divergence was as high as 39.3%. The polymerase chain reaction was used to amplify and clone long terminal repeat sequences from Th-1, the in vivo parental stock of MA-1. Results indicated that the nucleotide sequences of MA-1 and Th-1 clones were less variable than was observed between MA-1 and Wyoming. However, MA-1 and Th-1 markedly differed in the types of enhancer sequences located in the hypervariable region. These results suggest that variation in lentivirus regulatory sequences may be important in EIAV host cell tropism and pathogenesis. C1 NIAID,ROCKY MT LABS,PERSISTENT VIRAL DIS LAB,HAMILTON,MT 59840. RP CARPENTER, S (reprint author), IOWA STATE UNIV SCI & TECHNOL,DEPT VET MICROBIOL & PREVENT MED,AMES,IA 50011, USA. OI Alexandersen, Soren/0000-0002-5039-3178 NR 36 TC 49 Z9 51 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD MAR PY 1991 VL 65 IS 3 BP 1605 EP 1610 PG 6 WC Virology SC Virology GA EY750 UT WOS:A1991EY75000067 PM 1847479 ER PT J AU GESSAIN, A LOUIE, A GOUT, O GALLO, RC FRANCHINI, G AF GESSAIN, A LOUIE, A GOUT, O GALLO, RC FRANCHINI, G TI HUMAN T-CELL LEUKEMIA-LYMPHOMA VIRUS TYPE-I (HTLV-I) EXPRESSION IN FRESH PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM PATIENTS WITH TROPICAL SPASTIC PARAPARESIS HTLV-I-ASSOCIATED MYELOPATHY SO JOURNAL OF VIROLOGY LA English DT Note ID LYMPHOTROPIC VIRUS; HUMAN RETROVIRUS; MESSENGER-RNA; ACTIVATED LYMPHOCYTES; CEREBROSPINAL-FLUID; NATURAL ANTIBODIES; C RETROVIRUS; TAT GENE; INTERLEUKIN-2; INDUCTION AB Tropical spastic paraparesis/human T-cell leukemia-lymphoma virus type I (HTLV-I)-associated myelopathy (TSP/HAM) is a chronic neurological illness epidemiologically associated with HTLV-I infection. We investigated the role of HTLV-I in the pathogenesis of this disease by studying viral expression in fresh uncultured peripheral blood mononuclear cells (PBMCs) of six patients of Caribbean origin with TSP/HAM. The PBMC genomic DNA of all the patients studied carried HTLV-I provirus, but viral expression was not detected by Northern (RNA) blot analysis of total cellular PBMC RNA. When the reverse transcriptase polymerase chain reaction technique was used with primers specific for the tax-rex mRNA, all of the samples were positive for this viral mRNA species, regardless of the duration of the illness (range, 2 to 13 years). The splice junctions for the tax-rex mRNA described in cases of HTLV-I-induced adult T-cell leukemia (position 5183 of the envelope and position 7302 of the pX region) were identical in three TSP/HAM cases studied. To ascertain whether viral expression occurred at a low level in many cells or at a high level in a few permissive cells, we performed in situ hybridization on fresh PBMCs from two patients (2 and 7 years after clinical diagnosis), seeking HTLV-I RNA sequences. Our finding indicated that in vivo HTLV-I expression occurred at a high level in a few cells (1 of every 5,000 PBMCs) in both cases studied. The fact that cells of all six patients with TSP/HAM were positive for viral expression, regardless of the time lag from diagnosis, suggests that persistent expression of a viral product(s) may be pivotal in the pathogenesis of TSP/HAM. C1 NCI,TUMOR CELL BIOL LAB,BETHESDA,MD 20892. SALPETRIERE HOSP,DEPT NEUROL & NEUROPSYCHOL,PARIS,FRANCE. NR 47 TC 86 Z9 86 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD MAR PY 1991 VL 65 IS 3 BP 1628 EP 1633 PG 6 WC Virology SC Virology GA EY750 UT WOS:A1991EY75000072 PM 1995955 ER PT J AU CONNORS, M COLLINS, PL FIRESTONE, CY MURPHY, BR AF CONNORS, M COLLINS, PL FIRESTONE, CY MURPHY, BR TI RESPIRATORY SYNCYTIAL VIRUS (RSV) F-PROTEIN, G-PROTEIN, M2-PROTEIN (22K), AND N-PROTEINS EACH INDUCE RESISTANCE TO RSV CHALLENGE, BUT RESISTANCE INDUCED BY M2-PROTEINS AND N-PROTEINS IS RELATIVELY SHORT-LIVED SO JOURNAL OF VIROLOGY LA English DT Note ID RECOMBINANT VACCINIA VIRUS; COTTON RATS; MONOCLONAL-ANTIBODIES; FUSION PROTEIN; G-GLYCOPROTEIN; HOST IMMUNITY; INFECTION; EXPRESSION; MICE; IMMUNIZATION AB The ability of recombinant vaccinia viruses that separately encoded 9 of the 10 known respiratory syncytial virus (RSV) proteins to induce resistance to RSV challenge was studied in BALB/c mice. Resistance was examined at two intervals following vaccination to examine early (day 9) as well as late (day 28) immunity. BALB/c mice were inoculated simultaneously by the intranasal and intraperitoneal routes with a recombinant vaccinia virus encoding one of the following RSV proteins: F, G, N, P, SH, M, 1B, 1C, or M2 (22K). A parainfluenza virus type 3 HN protein recombinant (Vac-HN) served as a negative control. One half of the mice were challenged with RSV intranasally on day 9, and the remaining animals were challenged on day 28 postvaccination. Mice previously immunized by infection with RSV, Vac-F, or Vac-G were completely or almost completely resistant to RSV challenge on both days. In contrast, immunization with Vac-HN, -P, -SH, -M, -1B, or -1C did not induce detectable resistance to RSV challenge. Mice previously infected with Vac-M2 or Vac-N exhibited significant but not complete resistance on day 9. However, in both cases resistance had largely waned by day 28 and was detectable only in mice immunized with Vac-M2. These results demonstrate that F and G proteins expressed by recombinant vaccinia viruses are the most effective RSV protective antigens. This study also suggests that RSV vaccines need only contain the F and G glycoproteins, because the immunity conferred by the other proteins is less effective and appears to wane rapidly with time. RP CONNORS, M (reprint author), NIAID,INFECT DIS LAB,BLDG 7,ROOM 100,BETHESDA,MD 20892, USA. NR 23 TC 148 Z9 149 U1 1 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD MAR PY 1991 VL 65 IS 3 BP 1634 EP 1637 PG 4 WC Virology SC Virology GA EY750 UT WOS:A1991EY75000073 PM 1995956 ER PT J AU LIPRANDI, F RODRIGUEZ, I PINA, C LARRALDE, G GORZIGLIA, M AF LIPRANDI, F RODRIGUEZ, I PINA, C LARRALDE, G GORZIGLIA, M TI VP4-MONOTYPE SPECIFICITIES AMONG PORCINE ROTAVIRUS STRAINS OF THE SAME-VP4 SEROTYPE SO JOURNAL OF VIROLOGY LA English DT Note ID ANTIGENIC CHARACTERIZATION; NEUTRALIZATION EPITOPES; MONOCLONAL-ANTIBODIES; VP4 PROTEIN; GENE; OSU; IDENTIFICATION; INFECTIVITY; REACTIVITY AB The porcine rotavirus OSU strain was used to produce monoclonal antibodies (MAbs) directed against the outer capsid protein VP4. From two separate fusions, eight MAbs that inhibited hemagglutination activity of the OSU strain were selected. All MAbs immunoprecipitated both the OSU VP4 protein derived from a lysate of infected MA104 cells and the OSU VP4 protein expressed in Sf9 cells by a recombinant baculovirus. By immunoprecipitation of in vitro-translated OSU gene 4 transcripts of different length, the eight MAbs were found to be specific for the VP8 subunit of VP4. All MAbs neutralized the OSU strain but failed to neutralize human, bovine, and simian rotavirus strains. Antiserum to the expressed OSU VP4 protein was used to study the distribution of VP4 antigenicity among porcine rotaviruses. At least two distinct specificities were identified among 14 rotavirus strains that had been previously assigned to four distinct VP7 serotypes. Five groups of monotype specificities of the VP4 protein were identified by the eight anti-VP4 MAbs among 11 porcine strains that share the same VP4 serotype. C1 NIAID,INFECT DIS LAB,BETHESDA,MD 20892. RP LIPRANDI, F (reprint author), INST VENEZOLANO INVEST CIENT,BIOL VIRUS LAB,APARTADO 210287,CARACAS 1010A,VENEZUELA. NR 26 TC 33 Z9 33 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD MAR PY 1991 VL 65 IS 3 BP 1658 EP 1661 PG 4 WC Virology SC Virology GA EY750 UT WOS:A1991EY75000078 PM 1847483 ER PT J AU FERRUCCI, L GURALNIK, JM BARONI, A TESI, G ANTONINI, E MARCHIONNI, N AF FERRUCCI, L GURALNIK, JM BARONI, A TESI, G ANTONINI, E MARCHIONNI, N TI VALUE OF COMBINED ASSESSMENT OF PHYSICAL HEALTH AND FUNCTIONAL STATUS IN COMMUNITY-DWELLING AGED - A PROSPECTIVE-STUDY IN FLORENCE, ITALY SO JOURNALS OF GERONTOLOGY LA English DT Article ID MORTALITY; OUTCOMES; CAPACITY AB A survey of the health and social conditions of a representative sample of 967 persons aged 60 years and older from the city of Florence, Italy, was undertaken in 1980. In 1987, a follow-up survey of this cohort was performed. There were 391 documented deaths, 408 survivors, and 168 individuals who could not be located. Functional ability at baseline was assessed using a World Health Organization 14-item scale. Indicators of physical health status included chronic disease status, number of drugs, physician visits, and days of hospitalization. After adjustment for age and sex, both functional ability and indicators of physical health status were found to be independent, statistically significant predictors of mortality. The results of this study further support the view that biomedical and functional assessment are both necessary for a comprehensive evaluation of the older population. C1 NIA,1550 WISCONSIN AVE,FED BLDG,ROOM 618,BETHESDA,MD 20892. HOSP I FRATICINI,DEPT GERIATR & REHABIL,DEPT GERIAT,INRCA,FLORENCE,ITALY. NATL INST RES & CARE ELDERLY,FLORENCE,ITALY. UNIV FLORENCE,I-50121 FLORENCE,ITALY. NR 15 TC 40 Z9 41 U1 0 U2 1 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 0022-1422 J9 J GERONTOL JI J. Gerontol. PD MAR PY 1991 VL 46 IS 2 BP M52 EP M56 PG 5 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA GQ139 UT WOS:A1991GQ13900011 PM 1997573 ER PT J AU MEYERS, DA GOLDBERG, AP BLEECKER, ML COON, PJ DRINKWATER, DT BLEECKER, ER AF MEYERS, DA GOLDBERG, AP BLEECKER, ML COON, PJ DRINKWATER, DT BLEECKER, ER TI RELATIONSHIP OF OBESITY AND PHYSICAL-FITNESS TO CARDIOPULMONARY AND METABOLIC FUNCTION IN HEALTHY OLDER MEN SO JOURNALS OF GERONTOLOGY LA English DT Article ID DENSITY-LIPOPROTEIN CHOLESTEROL; SEDENTARY MEN; HEART-RATE; AGE; EXERCISE; CAPACITY; DISEASE; VO2MAX; SEX AB The relationship of obesity and physical fitness (VO2max) to cardiopulmonary and metabolic function was examined in 132 healthy obese, nonsmoking men age 45-79. Obese men with higher VO2max had lower % body fat and waist-to-hip ratio (WHR) than obese men with low VO2max. The obese subjects with high WHR (upper body fat distribution) had higher systolic blood pressure, hyperinsulinemia and impaired glucose tolerance, lower high density lipoprotein cholesterol (HDL-C), and higher triglyceride (TG). VO2max (ml/kg FFM.min) was lower in the older men (r = -0.54, p < .001), and 32% of the variation was accounted for by age and the one-second forced expiratory volume. Although pulmonary function was normal, 50% of the variability was predicted by age, height, and VO2max or WHR. Glucose tolerance and insulin correlated better with VO2max and indices of body composition than with age, while plasma TG and HDL-C correlated with body composition, not VO2max or age. Thus, while age affects the cardiopulmonary and metabolic function of obese older men, physical inactivity, obesity, and an abdominal body fat distribution (increased WHR) contributed significantly to their reductions in physiological function. C1 JOHNS HOPKINS UNIV,SCH MED,FRANCIS SCOTT KEY MED CTR,BALTIMORE,MD 21205. NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. FU NCRR NIH HHS [MO1 RR02719]; NIA NIH HHS [P01 AG-04402-05, 1 K08 AG-00347] NR 40 TC 42 Z9 42 U1 0 U2 1 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 0022-1422 J9 J GERONTOL JI J. Gerontol. PD MAR PY 1991 VL 46 IS 2 BP M57 EP M65 PG 9 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA GQ139 UT WOS:A1991GQ13900012 PM 1997574 ER PT J AU BACKER, TE SALASIN, SE RICH, RF AF BACKER, TE SALASIN, SE RICH, RF TI FEDERAL-POLICY AND KNOWLEDGE UTILIZATION - ISSUES FOR THE NEW DECADE SO KNOWLEDGE-CREATION DIFFUSION UTILIZATION LA English DT Article C1 UNIV CALIF LOS ANGELES,MED PSYCHOL,LOS ANGELES,CA 90024. NIMH,BETHESDA,MD 20892. UNIV ILLINOIS,INST GOVT & PUBL AFFAIRS,URBANA,IL 61801. RP BACKER, TE (reprint author), HUMAN INTERACT RES INST,LOS ANGELES,CA, USA. NR 1 TC 0 Z9 0 U1 1 U2 1 PU SAGE SCIENCE PRESS PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 SN 0164-0259 J9 KNOWLEDGE PD MAR PY 1991 VL 12 IS 3 BP 220 EP 224 PG 5 WC Social Sciences, Interdisciplinary SC Social Sciences - Other Topics GA FA476 UT WOS:A1991FA47600001 ER PT J AU DAVID, S AF DAVID, S TI DEVELOPING A TECHNOLOGY-TRANSFER PROGRAM FOR THE NATIONAL-INSTITUTE-ON-DRUG-ABUSE SO KNOWLEDGE-CREATION DIFFUSION UTILIZATION LA English DT Article RP DAVID, S (reprint author), NIDA,COMMUNITY & PROFESS EDUC BRANCH,ROCKVILLE,MD 20857, USA. NR 4 TC 2 Z9 2 U1 1 U2 1 PU SAGE SCIENCE PRESS PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 SN 0164-0259 J9 KNOWLEDGE PD MAR PY 1991 VL 12 IS 3 BP 289 EP 297 PG 9 WC Social Sciences, Interdisciplinary SC Social Sciences - Other Topics GA FA476 UT WOS:A1991FA47600005 ER PT J AU SHEN, RN LU, L HARRINGTON, MA SRIVASTAVA, C KIM, YJ ZHOU, SZ WU, B RUSCETTI, S BROXMEYER, HE AF SHEN, RN LU, L HARRINGTON, MA SRIVASTAVA, C KIM, YJ ZHOU, SZ WU, B RUSCETTI, S BROXMEYER, HE TI EFFECT OF SPLIT LOW-DOSE TOTAL-BODY IRRADIATION ON SFFV MESSENGER-RNA, GENOMIC DNA AND PROTEIN EXPRESSION IN MICE INFECTED WITH THE FRIEND-VIRUS COMPLEX SO LEUKEMIA LA English DT Article ID FOCUS-FORMING VIRUS; LEUKEMIA; TUMORS; CELLS AB DBA/2 mice infected with lethal dosages of Friend virus complex (FVC) can be 100% cured by split-dose total body irradiation (TBI) at 150 cGy, an effect associated with the restoration of the cellular immunity which is compromised by the virus. The exact mechanism underlying the curative effect is unknown, but it may involve the interferon (IFN) system and interleukin-2 (IL-2) production. Initial studies indicated that TBI did not directly inactivate the virus, suggesting that irradiation either acted on the target cells for virus replication or on other cells mediating the effect. We have now examined the effect of this relatively low dose TBI on replication, transcription, and protein expression of the Friend virus. Northern blot analysis revealed that in FVC infected mice treated with curative low dose TBI, no spleen focus-forming virus (SFFV)-specific mRNA species were detected. Southern blot analysis revealed that a 6.0 kb SFFV fragment could be detected in infected, untreated spleen cells, but not in cells from FVC-infected mice treated with TBI, or in uninfected spleen cells. Western blot analysis revealed that the SFFV envelope glycoprotein was expressed in the spleen cells from untreated FVC infected mice, but not in the cells from TBI treated FVC infected mice. These results, consistent with our previous findings of greatly reduced spleen focus forming units in mice with FVC which had been treated with this regimen of TBI, suggest the possibility of using such treatments in other retroviral associated disorders. C1 INDIANA UNIV,SCH MED,DEPT MICROBIOL IMMUNOL,INDIANAPOLIS,IN 46202. INDIANA UNIV,SCH MED,DEPT RADIAT ONCOL,INDIANAPOLIS,IN 46202. INDIANA UNIV,SCH MED,DEPT MED HEMATOL ONCOL,INDIANAPOLIS,IN 46202. NCI,FREDERICK CANC RES & DEV CTR,MOLEC ONCOL LAB,FREDERICK,MD 21701. RP SHEN, RN (reprint author), INDIANA UNIV,SCH MED,WALTHER ONCOL CTR,MED RES & LIB BLDG,975 W WALNUT ST,ROOM 501,INDIANAPOLIS,IN 46202, USA. FU NCI NIH HHS [CA 36740, CA 36464] NR 20 TC 6 Z9 7 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0887-6924 J9 LEUKEMIA JI Leukemia PD MAR PY 1991 VL 5 IS 3 BP 225 EP 229 PG 5 WC Oncology; Hematology SC Oncology; Hematology GA FL312 UT WOS:A1991FL31200009 PM 2013981 ER PT J AU LYON, RC PEKAR, J MOONEN, CTW MCLAUGHLIN, AC AF LYON, RC PEKAR, J MOONEN, CTW MCLAUGHLIN, AC TI DOUBLE-QUANTUM SURFACE-COIL NMR-STUDIES OF SODIUM AND POTASSIUM IN THE RAT-BRAIN SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article ID NUCLEAR MAGNETIC-RESONANCE; COHERENCE; TISSUE; NA-23; COMPARTMENTALIZATION; RELAXATION; SELECTION; PROBE C1 NIH,INVIVO NMR RES CTR,BIOMED ENGN & INSTRUMENTAT PROGRAM,BLDG 10,BETHESDA,MD 20892. NIAAA,METAB & MOLEC BIOL LAB,ROCKVILLE,MD 20852. RI Moonen, Chrit/K-4434-2016 OI Moonen, Chrit/0000-0001-5593-3121 NR 31 TC 57 Z9 57 U1 0 U2 4 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0740-3194 J9 MAGNET RESON MED JI Magn.Reson.Med. PD MAR PY 1991 VL 18 IS 1 BP 80 EP 92 DI 10.1002/mrm.1910180110 PG 13 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EZ547 UT WOS:A1991EZ54700009 PM 2062244 ER PT J AU FRALIX, TA CECKLER, TL WOLFF, SD SIMON, SA BALABAN, RS AF FRALIX, TA CECKLER, TL WOLFF, SD SIMON, SA BALABAN, RS TI LIPID BILAYER AND WATER PROTON MAGNETIZATION TRANSFER - EFFECT OF CHOLESTEROL SO MAGNETIC RESONANCE IN MEDICINE LA English DT Note ID CROSS-RELAXATION; PHOSPHOLIPID PACKING; SPIN RELAXATION; RESONANCE; VESICLES; NMR; T2 C1 DUKE UNIV,MED CTR,DEPT NEUROBIOL & ANESTHESIOL,DURHAM,NC 27710. RP FRALIX, TA (reprint author), NHLBI,CARDIAC ENERGET LAB,BETHESDA,MD 20892, USA. RI Balaban, Robert/A-7459-2009 OI Balaban, Robert/0000-0003-4086-0948 FU NINDS NIH HHS [NS 20669] NR 28 TC 84 Z9 85 U1 1 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0740-3194 J9 MAGNET RESON MED JI Magn.Reson.Med. PD MAR PY 1991 VL 18 IS 1 BP 214 EP 223 DI 10.1002/mrm.1910180122 PG 10 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EZ547 UT WOS:A1991EZ54700021 PM 2062233 ER PT J AU BERNSTEIN, AB THOMPSON, GB HARLAN, LC AF BERNSTEIN, AB THOMPSON, GB HARLAN, LC TI DIFFERENCES IN RATES OF CANCER SCREENING BY USUAL SOURCE OF MEDICAL-CARE - DATA FROM THE 1987 NATIONAL-HEALTH INTERVIEW SURVEY SO MEDICAL CARE LA English DT Article ID BREAST-CANCER; COLORECTAL-CANCER; PERFORMANCE; MORTALITY; SERVICES; TRENDS; TRIAL; ADULT AB Data from the Cancer Control Supplement of the 1987 Health Interview Survey (NHIS), a nationally representative sample of the United States population, was used to examine rates of six screening tests for cancer. The rates of screening were compared for people who receive their usual source of medical care in an HMO or prepaid group practice versus those who receive it in the fee-for-service sector. The purpose of this article is to determine whether HMOs remain more likely to offer cancer screening examinations than the fee-for-service sector. Results indicate that for five of six screening tests examined (Pap smear, mammography, breast physical examination, digital rectal examination, and blood stool test), members of HMOs are significantly more likely to have received the test within the last 3-year period. These results hold in a multivariate analysis when many factors correlated with selection into HMOs, health status, and use of medical services are controlled for, although results are only generalizable to whites. Future research should focus on why the rates for five of the six cancer screening tests examined are higher in HMO settings, and how we can use the HMO experience to improve consensus as to the usefulness of the tests in the non-HMO physician pool, and ultimately increase rates of screening tests in the non-HMO population. C1 NATL CTR HLTH STAT,HYATTSVILLE,MD 20782. NCI,BETHESDA,MD 20892. RP BERNSTEIN, AB (reprint author), PROSPECT PAYMENT ASSESSMENT COMMISS,300 7TH ST SW,SUITE 301B,WASHINGTON,DC 20024, USA. NR 28 TC 87 Z9 87 U1 0 U2 5 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD MAR PY 1991 VL 29 IS 3 BP 196 EP 209 PG 14 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA FB706 UT WOS:A1991FB70600002 PM 1997750 ER PT J AU BULLER, RML PALUMBO, GJ AF BULLER, RML PALUMBO, GJ TI POXVIRUS PATHOGENESIS SO MICROBIOLOGICAL REVIEWS LA English DT Review ID VIRUS-INFECTED CELLS; SHOPE FIBROMA VIRUS; EPIDERMAL GROWTH-FACTOR; GENERALIZED VIRAL-INFECTION; MICE INNATELY RESISTANT; NATURAL-KILLER CELLS; DOUBLE-STRANDED-RNA; CYTOTOXIC T-CELLS; DELAYED-TYPE HYPERSENSITIVITY; MEDIATED IMMUNE-RESPONSE AB Poxviruses are a highly successful family of pathogens, with variola virus, the causative agent of smallpox, being the most notable member. Poxviruses are unique among animal viruses in several respects. First, owing to the cytoplasmic site of virus replication, the virus encodes many enzymes required either for macromolecular precursor pool regulation or for biosynthetic processes. Second, these viruses have a very complex morphogenesis, which involves the de novo synthesis of virus-specific membranes and inclusion bodies. Third, and perhaps most surprising of all, the genomes of these viruses encode many proteins which interact with host processes at both the cellular and systemic levels. For example, a viral homolog of epidermal growth factor is active in vaccinia virus infections of cultured cells, rabbits, and mice. At least five virus proteins with homology to the serine protease inhibitor family have been identified and one, a 38-kDa protein encoded by cowpox virus, is thought to block a host pathway for generating a chemotactic substance. Finally, a protein which has homology with complement components interferes with the activation of the classical complement pathway. Poxviruses infect their hosts by all possible routes: through the skin by mechanical means (e.g., molluscum contagiosum infections of humans), via the respiratory tract (e.g., variola virus infections of humans), or by the oral route (e.g., ectromelia virus infection of the mouse). Poxvirus infections, in general, are acute, with no strong evidence for latent, persistent, or chronic infections. They can be localized or systemic. Ectromelia virus infection of the laboratory mouse can be systemic but inapparent with no mortality and little morbidity, or highly lethal with death in 10 days. On the other hand, molluscum contagiosum virus replicates only in the stratum spinosum of the human epidermis, with little or no involvement of the dermis, and does not spread systemically from the site of infection. The host response to infection is progressive and multifactorial. Early in the infection process, interferons, the alternative pathway of complement activation, inflammatory cells, and natural killer cells may contribute to slowing the spread of the infection. The cell-mediated response involving learned cytotoxic T lymphocytes and delayed-type hypersensitivity components appears to be the most important in recovery from infection. A significant role for specific antiviral antibody and antibody-dependent cell-mediated cytotoxicity has yet to be demonstrated in recovery from a primary infection, but these responses are thought to be important in preventing reinfection. RP BULLER, RML (reprint author), NIAID,VIRAL DIS LAB,BETHESDA,MD 20892, USA. NR 538 TC 345 Z9 351 U1 3 U2 9 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0146-0749 J9 MICROBIOL REV JI Microbiol. Rev. PD MAR PY 1991 VL 55 IS 1 BP 80 EP 122 PG 43 WC Microbiology SC Microbiology GA FA695 UT WOS:A1991FA69500005 PM 1851533 ER PT J AU KRAGEL, PJ WALTHER, MM PESTANER, JP FILLINGKATZ, MR AF KRAGEL, PJ WALTHER, MM PESTANER, JP FILLINGKATZ, MR TI SIMPLE RENAL CYSTS, ATYPICAL RENAL CYSTS, AND RENAL-CELL CARCINOMA IN VON HIPPEL-LINDAU DISEASE - A LECTIN AND IMMUNOHISTOCHEMICAL STUDY IN 6 PATIENTS SO MODERN PATHOLOGY LA English DT Article DE SIMPLE RENAL CYST; ATYPICAL RENAL CYST; RENAL CELL CARCINOMA; PROXIMAL TUBULE; DISTAL TUBULE ID KIDNEY; MANAGEMENT AB We compare the expression of four markers of renal tubular differentiation in six renal cell carcinomas, five atypical renal cysts, and five simple renal cysts from six patients with von Hippel-Lindau disease. Proximal tubular markers were expressed by five of six renal cell carcinomas, three of five atypical renal cysts, and zero of five simple renal cysts. Distal tubular markers were expressed by one of six renal cell carcinomas, five of five atypical renal cysts, and four of five simple renal cysts. One of the three atypical cysts which expressed distal tubular markers was associated with a renal cell carcinoma which also expressed distal tubular markers. Our findings suggest that simple renal cysts in von Hippel-Lindau disease arise more commonly from distal rather than proximal tubules, while atypical renal cysts show tubular origin similar to renal cell carcinomas. C1 NCI,PATHOL LAB,BETHESDA,MD 20892. NCI,SURG BRANCH,BETHESDA,MD 20892. GREATER BALTIMORE MED CTR,DEPT MED,TOWSON,MD. UNIV LOUISVILLE,DEPT OPHTHALMOL & NEUROL,LOUISVILLE,KY 40292. NR 16 TC 26 Z9 26 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD MAR PY 1991 VL 4 IS 2 BP 210 EP 214 PG 5 WC Pathology SC Pathology GA FD775 UT WOS:A1991FD77500015 PM 2047384 ER PT J AU DERRICO, A GARBISA, S LIOTTA, LA CASTRONOVO, V STETLERSTEVENSON, WG GRIGIONI, WF AF DERRICO, A GARBISA, S LIOTTA, LA CASTRONOVO, V STETLERSTEVENSON, WG GRIGIONI, WF TI AUGMENTATION OF TYPE-IV COLLAGENASE, LAMININ RECEPTOR, AND KI67 PROLIFERATION ANTIGEN ASSOCIATED WITH HUMAN COLON, GASTRIC, AND BREAST-CARCINOMA PROGRESSION SO MODERN PATHOLOGY LA English DT Article DE TUMOR PROGRESSION; BASEMENT MEMBRANES; COLLAGENASE; LAMININ; KI67 ID BASEMENT-MEMBRANE COLLAGEN; EXPRESSION; CELLS; CANCER AB The proportion of neoplastic cells immunocytochemically positive for type IV collagenase (IVase), laminin receptor (LR), and Ki67 proliferation-associated antigen increased during the progression of human colon, gastric, and breast carcinomas. Thirty cases of colonic adenoma were compared with 30 cases of Dukes' A or B stage carcinoma and ten cases of Dukes' C stage carcinoma. The percentage of positive cells increased significantly (P < 0.001) for all three antigens comparing carcinomas with adenomas and Dukes' C stage compared with Dukes' A/B stage. The same pattern of antigen correlation with progression was found with 40 human gastric carcinomas. Gastric carcinomas classified as well-differentiated advanced stage contained a significantly higher proportion of tumor cells positive for IVase (P < 0.001), LR (P < 0.001), and Ki67 (P < 0.001) compared with well-differentiated superficial tumors. Gastric carcinomas classified as poorly differentiated superficial had a significantly higher proportion of cells positive for Ki67 (P < 0.016), but not IVase (P < 0.069) or LR (P < 0.075), compared with poorly differentiated advanced tumors. Metastasis of colon and gastric carcinoma retained the immunostaining pattern of the primary tumors. Thirty cases of breast neoplasia were compared with 30 adjacent samples of normal duct epithelium. A positive correlation (P < 0.001) was found for the immunoreactivity of all three antigens in the invasive carcinomas compared with the normal epithelium. Invasive ductal carcinoma and invasive lobular carcinoma had a significantly higher percentage of immunoreactivity for the three antigens compared with corresponding in situ lesions. In addition to a higher rate of proliferation, the augmented levels of IVase, and LR, which are known to play a role in the interaction of cells with the basement membrane, may reflect an augmented propensity to invade basement membranes during tumor progression. C1 NCI,PATHOL LAB,BLDG 10,ROOM 2A33,9000 ROCKVILLE PK,BETHESDA,MD 20892. IST ISTOL & EMBRIOL,PADUA,ITALY. UNIV BOLOGNA,IST ANAT PATOL,I-40126 BOLOGNA,ITALY. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 27 TC 221 Z9 221 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD MAR PY 1991 VL 4 IS 2 BP 239 EP 246 PG 8 WC Pathology SC Pathology GA FD775 UT WOS:A1991FD77500020 PM 1646457 ER PT J AU TURCO, SJ SACKS, DL AF TURCO, SJ SACKS, DL TI EXPRESSION OF A STAGE-SPECIFIC LIPOPHOSPHOGLYCAN IN LEISHMANIA-MAJOR AMASTIGOTES SO MOLECULAR AND BIOCHEMICAL PARASITOLOGY LA English DT Article DE LIPOPHOSPHOGLYCAN; LEISHMANIA; AMASTIGOTE; GLYCOCONJUGATE ID KINASE-C ACTIVITY; DONOVANI LIPOPHOSPHOGLYCAN; PROMASTIGOTES; MACROPHAGES AB Amastigotes of Leishmania major were isolated from infected mice and radiolabeled for 2 h with [H-3]galactose. An acidic [H-3]glycoconjugate was extracted from a dilipidated residue fraction with the solvent water/ethanol/diethylether/pyridine/NH4OH (15:15:5:1:0.017). The radioactively labeled glycoconjugate was found to possess the following characteristics that were similar to the lipophosphoglycan extractable from promastigotes: (i) migrated as a broad band upon electrophoresis on SDS polyacrylamide gels; (ii) deaminated with nitrous acid; and (iii) hydrolyzed with phosphatidylinositol-specific phospholipase C. Furthermore, analysis of the aqueous soluble material released by the latter enzyme revealed a negatively-charged [H-3]polysaccharide intermediate in size compared to the analogous portions of LPG isolated from non-infective and metacyclic promastigotes. Most importantly, the [H-3]polysaccharide was found to contain phosphate and was susceptible to mild acid hydrolysis, establishing that the intact molecule is a lipophosphoglycan. A structural difference, however, was found in the major, mild acid-generated fragment of the amastigote phosphoglycan, which was larger in size and not as anionic as the analogous fragment from the promastigote phosphoglycans. These results indicate that the amastigotes do express a lipophosphoglycan, but that it is structurally distinct from its promastigote counterparts. C1 NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. RP TURCO, SJ (reprint author), UNIV KENTUCKY,DEPT BIOCHEM,LEXINGTON,KY 40536, USA. FU NIAID NIH HHS [AI20941] NR 25 TC 80 Z9 80 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-6851 J9 MOL BIOCHEM PARASIT JI Mol. Biochem. Parasitol. PD MAR PY 1991 VL 45 IS 1 BP 91 EP 100 DI 10.1016/0166-6851(91)90030-A PG 10 WC Biochemistry & Molecular Biology; Parasitology SC Biochemistry & Molecular Biology; Parasitology GA EY721 UT WOS:A1991EY72100010 PM 1646960 ER PT J AU WHITESELL, L ROSOLEN, A NECKERS, LM AF WHITESELL, L ROSOLEN, A NECKERS, LM TI EPISOME-GENERATED N-MYC ANTISENSE RNA RESTRICTS THE DIFFERENTIATION POTENTIAL OF PRIMITIVE NEUROECTODERMAL CELL-LINES SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID HUMAN NEURO-BLASTOMA; EPSTEIN-BARR VIRUS; GENE-PRODUCT; EXPRESSION; ONCOGENE; AMPLIFICATION; NEUROBLASTOMAS AB Neuroectodermal tumors of childhood provide a unique opportunity to examine the role of genes potentially regulating neuronal growth and differentiation because many cell lines derived from these tumors are composed of at least two distinct morphologic cell types. These types display variant phenotypic characteristics and spontaneously interconvert, or transdifferentiate, in vitro. The factors that regulate transdifferentiation are unknown. Application of antisense approaches to the transdifferentiation process has allowed us to explore the precise role that N-myc may play in regulating developing systems. We now report construction of an episomally replicating expression vector designed to generate RNA antisense to part of the human N-myc gene. Such a vector is able to specifically inhibit N-myc expression in cell lines carrying both normal and amplified N-myc alleles. Inhibition of N-myc expression blocks transdifferentiation in these lines, with accumulation of cells of an intermediate phenotype. A concomitant decrease in growth rate but not loss of tumorigenicity was observed in the N-myc nonamplified cell line CHP-100. Vector-generated antisense RNA should allow identification of genes specifically regulated by the proto-oncogene N-myc. RP WHITESELL, L (reprint author), NCI,CLIN PHARMACOL BRANCH,TUMOR CELL BIOL SECT,BETHESDA,MD 20892, USA. FU NICHD NIH HHS [N01-HD-6-2915] NR 32 TC 50 Z9 51 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD MAR PY 1991 VL 11 IS 3 BP 1360 EP 1371 PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA EZ331 UT WOS:A1991EZ33100019 PM 1996098 ER PT J AU NUELL, MJ STEWART, DA WALKER, L FRIEDMAN, V WOOD, CM OWENS, GA SMITH, JR SCHNEIDER, EL DELLORCO, R LUMPKIN, CK DANNER, DB MCCLUNG, JK AF NUELL, MJ STEWART, DA WALKER, L FRIEDMAN, V WOOD, CM OWENS, GA SMITH, JR SCHNEIDER, EL DELLORCO, R LUMPKIN, CK DANNER, DB MCCLUNG, JK TI PROHIBITIN, AN EVOLUTIONARILY CONSERVED INTRACELLULAR PROTEIN THAT BLOCKS DNA-SYNTHESIS IN NORMAL FIBROBLASTS AND HELA-CELLS SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID RETINOBLASTOMA SUSCEPTIBILITY GENE; TUMOR SUPPRESSOR GENES; MESSENGER-RNA; MICROINJECTION SYSTEM; MOLECULAR-CLONING; GROWTH ARREST; RAT-LIVER; EXPRESSION; SEQUENCE; CDNA AB Genes that act inside the cell to negatively regulate proliferation are of great interest because of their implications for such processes as development and cancer, but these genes have been difficult to clone. This report details the cloning and analysis of cDNA for prohibitin, a novel mammalian antiproliferative protein. Microinjection of synthetic prohibitin mRNA blocks entry into S phase in both normal fibroblasts and HeLa cells. Microinjection of an antisense oligonucleotide stimulates entry into S phase. By sequence comparison, the prohibitin gene appears to be the mammalian analog of Cc, a Drosophila gene that is vital for normal development. C1 NIA,MOLEC GENET LAB,4940 EASTERN AVE,BALTIMORE,MD 21224. SR NOBLE FDN,DIV BIOMED,ARDMORE,OK 73401. BAYLOR UNIV,HUFFINGTON CTR AGING,DEPT CELL BIOL,DIV MOLEC VIROL,HOUSTON,TX 77030. BAYLOR UNIV,DEPT MED,HOUSTON,TX 77030. UNIV SO CALIF,ANDRUS GERONTOL CTR,LOS ANGELES,CA 90089. UNIV ARKANSAS MED SCI HOSP,DEPT PEDIAT,LITTLE ROCK,AR 72205. FU NIAAA NIH HHS [AA 07550-03] NR 51 TC 209 Z9 220 U1 2 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD MAR PY 1991 VL 11 IS 3 BP 1372 EP 1381 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA EZ331 UT WOS:A1991EZ33100020 PM 1996099 ER PT J AU ROTH, HJ DAS, GC PIATIGORSKY, J AF ROTH, HJ DAS, GC PIATIGORSKY, J TI CHICKEN BETA-B1-CRYSTALLIN GENE-EXPRESSION - PRESENCE OF CONSERVED FUNCTIONAL POLYOMAVIRUS ENHANCER-LIKE AND OCTAMER BINDING-LIKE PROMOTER ELEMENTS FOUND IN NONLENS GENES SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID A-CRYSTALLIN GENE; TISSUE-SPECIFIC EXPRESSION; TRANSCRIPTION FACTOR; CHLORAMPHENICOL ACETYLTRANSFERASE; DELTA-CRYSTALLIN; TRANSGENIC MICE; DELTA-1-CRYSTALLIN GENE; SEQUENCE ELEMENTS; IMMUNOGLOBULIN GENES; REGULATORY ELEMENTS AB Expression of the chicken beta-B1-crystalline gene was examined. Northern (RNA) blot and primer extension analyses showed that while abundant in the lens, the beta-B1 mRNA is absent from the liver, brain, heart, skeletal muscle, and fibroblasts of the chicken embryo, suggesting lens specificity. Promoter fragments ranging from 434 to 126 bp of 5'-flanking sequence (plus 30 bp of exon 1) of the beta-B1 gene fused to the bacterial chloramphenicol acetyltransferase gene functioned much more efficiently in transfected embryonic chicken lens epithelial cells than in transfected primary muscle fibroblasts or HeLa cells. Transient expression of recombinant plasmids in cultured lens cells, DNase I footprinting, in vitro transcription in a HeLa cell extract, and gel mobility shift assays were used to identify putative functional promoter elements of the beta-B1-crystallin gene. Sequence analysis revealed a number of potential regulatory elements between positions -126 and -53 of the beta-B1 promoter, including two Sp1 sites, two octamer binding sequence-like sites (OL-1 and OL-2), and two polyomavirus enhancer-like sites (PL-1 and PL-2). Deletion and site-specific mutation experiments established the functional importance of PL-1 (-116 to -102), PL-2 (-90 to -76), and OL-2 (-75 to -68). DNase I footprinting using a lens or a HeLa cell nuclear extract and gel mobility shifts using a lens nuclear extract indicated the presence of putative lens transcription factors binding to these DNA sequences. Competition experiments provided evidence that PL-1 and PL-2 recognize the same or very similar factors, while OL-2 recognizes a different factor. Our data suggest that the same or closely related transcription factors found in many tissues are used for expression of the chicken beta-B1-crystalline gene in the lens. RP ROTH, HJ (reprint author), NEI,MOLEC & DEV BIOL LAB,BETHESDA,MD 20892, USA. NR 73 TC 43 Z9 43 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD MAR PY 1991 VL 11 IS 3 BP 1488 EP 1499 PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA EZ331 UT WOS:A1991EZ33100033 PM 1996106 ER PT J AU GUTKIND, JS LINK, DC KATAMINE, S LACAL, P MIKI, T LEY, TJ ROBBINS, KC AF GUTKIND, JS LINK, DC KATAMINE, S LACAL, P MIKI, T LEY, TJ ROBBINS, KC TI A NOVEL C-FGR EXON UTILIZED IN EPSTEIN-BARR VIRUS-INFECTED B LYMPHOCYTES BUT NOT IN NORMAL MONOCYTES SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID FELINE SARCOMA-VIRUS; PROTO-ONCOGENE; LCK TRANSCRIPTS; CELL-LINES; T-CELLS; EXPRESSION; PRODUCT; KINASE; GENE AB The fgr proto-oncogene encodes a nonreceptor protein-tyrosine kinase, designated p55c-fgr. In this study, we have isolated human fgr cDNA molecules from normal monocyte mRNA templates. Nucleotide sequence analysis of the longest fgr cDNA revealed a 5' untranslated region of 927 bp which included two Alu-like repeats as well as three translation stop codons immediately upstream of the initiator for p55c-fgr synthesis. Within genomic DNA, these sequences were distributed over 13 kbp as three distinct 5' untranslated exons. Previous studies have shown that Epstein-Barr virus (EBV) increases c-fgr mRNA levels in B lymphocytes. By comparing the nucleotide sequence reported for transcripts isolated from EBV-infected B lymphocytes with those of our monocyte cDNA as well as genomic DNA, we identified a novel untranslated exon utilized only in EBV-infected cells. The transcriptional initiation sites of fgr mRNA expressed in EBV-converted cells were mapped and shown to reside within a region identified as an intron for fgr mRNA that is expressed in normal myelomonocytic cells. Furthermore, the region of the fgr locus upstream of the novel exon displayed properties of a transcriptional promoter when transfected into heterologous cells. We conclude from all of these findings that activation of the fgr gene by EBV is achieved by mechanisms distinct from those normally regulating its programmed expression in myelomonocytic cells. C1 NIDR,CELLULAR DEV & ONCOL LAB,BETHESDA,MD 20892. NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892. WASHINGTON UNIV,JEWISH HOSP ST LOUIS,MED CTR,DEPT MED,DIV HEMATOL ONCOL,ST LOUIS,MO 63110. WASHINGTON UNIV,JEWISH HOSP ST LOUIS,MED CTR,DEPT GENET,ST LOUIS,MO 63110. RI Lacal, Pedro/A-1481-2013 OI Lacal, Pedro/0000-0003-1166-4593 NR 28 TC 21 Z9 24 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD MAR PY 1991 VL 11 IS 3 BP 1500 EP 1507 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA EZ331 UT WOS:A1991EZ33100034 PM 1847500 ER PT J AU DEBINSKI, W SIEGALL, CB FITZGERALD, D PASTAN, I AF DEBINSKI, W SIEGALL, CB FITZGERALD, D PASTAN, I TI SUBSTITUTION OF FOREIGN PROTEIN SEQUENCES INTO A CHIMERIC TOXIN COMPOSED OF TRANSFORMING GROWTH FACTOR-ALPHA AND PSEUDOMONAS EXOTOXIN SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID RECOMBINANT FUSION PROTEIN; AERUGINOSA; DOMAINS; ALPHA; CELLS AB TGF-alpha-PE40 is a chimeric toxin made by replacing domain Ia of Pseudomonas exotoxin (PE) with transforming growth factor-alpha (TGF-alpha). We have now replaced a portion of domain Ib of PE with different polypeptides or an extra domain III of PE in transforming growth factor alpha-PE40 and maintained cell killing. Thus, TGF-alpha-PE40 can be used to transport foreign protein sequences into the cytosol of cells. C1 NCI,DIV CANC BIOL & DIAGNOSIS,MOLEC BIOL LAB,BLDG 37,ROOM 4E16,BETHESDA,MD 20892. NCI CTR,BETHESDA,MD 20892. NR 16 TC 18 Z9 18 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD MAR PY 1991 VL 11 IS 3 BP 1751 EP 1753 PG 3 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA EZ331 UT WOS:A1991EZ33100065 PM 1671711 ER PT J AU NISSLEY, P LEE, L KIESS, W AF NISSLEY, P LEE, L KIESS, W TI EVIDENCE AGAINST A ROLE FOR INSULIN-LIKE GROWTH FACTOR-II IN THE AUTONOMOUS GROWTH OF RAT 18,54-SF CELLS SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Article DE INSULIN-LIKE GROWTH FACTOR-I; INSULIN-LIKE GROWTH FACTOR-II; INSULIN-LIKE GROWTH FACTOR-II MANNOSE 6-PHOSPHATE RECEPTOR; (18,54-SF CELL LINE) ID MULTIPLICATION-STIMULATING ACTIVITY; EMBRYO FIBROBLASTS; CONDITIONED MEDIUM; FACTOR RECEPTOR; IGF RECEPTOR; ANTIBODY; BINDING; LINE; IDENTIFICATION; PURIFICATION AB The 18,54-SF rat cell line multiplies in serum-free medium and has been reported to produce insulin-like growth factor II (IGF-II) and to possess IGF-II/mannose 6-phosphate (Man-6-P) receptors, raising the possibility of autocrine growth stimulation by IGF-II acting through this receptor. When serum-free medium was changed every 24 h the 18,54-SF cells multiplied at the same rate as when the medium was not changed. An antibody (No. 3637) which blocks the binding of IGF-II to the IGF-II/Man-6-P receptor did not decrease or increase the growth rate of the 18,54-SF cells when medium was changed every 24 h. When the medium was changed every 12 h the rate of accumulation of cells in the culture was decreased. Addition of IGF-I or IGF-II at 10, 50, and 100 ng/ml every 12 h did not correct this decrease in cell number. When the medium was replaced every 12 h, cells in the periphery of the cultures gradually became nonviable as assessed by trypan blue uptake. However, the percentage of cells synthesizing DNA in the center of the cultures (approximately 50% during a 3 h pulse with tritiated thymidine) was the same whether the medium was changed every 12 h or left unchanged. Addition of IGF-I or IGF-II to the fresh medium change every 12 h did not increase the percentage of cells synthesizing DNA. C1 UNIV MUNICH,CHILDRENS HOSP,W-8000 MUNICH 2,GERMANY. RP NISSLEY, P (reprint author), NCI,METAB BRANCH,ENDOCRINOL SECT,BLDG 10,RM 4N115,BETHESDA,MD 20892, USA. NR 27 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD MAR PY 1991 VL 75 IS 3 BP 213 EP 219 DI 10.1016/0303-7207(91)90163-M PG 7 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA FA686 UT WOS:A1991FA68600005 PM 1851112 ER PT J AU USALA, SJ MENKE, JB WATSON, TL WONDISFORD, FE WEINTRAUB, BD BERARD, J BRADLEY, WEC ONO, S MUELLER, OT BERCU, BB AF USALA, SJ MENKE, JB WATSON, TL WONDISFORD, FE WEINTRAUB, BD BERARD, J BRADLEY, WEC ONO, S MUELLER, OT BERCU, BB TI A HOMOZYGOUS DELETION IN THE C-ERBA-BETA THYROID-HORMONE RECEPTOR GENE IN A PATIENT WITH GENERALIZED THYROID-HORMONE RESISTANCE - ISOLATION AND CHARACTERIZATION OF THE MUTANT RECEPTOR SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID RESPONSE ELEMENT; BINDING; IDENTIFICATION; PROTEIN; TRANSCRIPTION; MUTATION; DOMAIN; SYSTEM AB Different point mutations have been identified in the T3-binding domain of the c-erbA-beta thyroid hormone receptor gene that are associated with variant phenotypes of generalized thyroid hormone resistance (GTHR). In most cases of GTHR, heterozygotes are affected; a single mutant allele results in the inhibition of the function of normal thyroid hormone receptors. We report here a novel genetic abnormality, a 3-basepair (bp) deletion in the T3-binding domain of the beta-receptor in a kindred, S, with GTHR. One patient, S1, was the product of a consanguineous union of two heterozygotes and was homozygous for this defect. Heterozygotes from kindred S harbored a CAC deletion at nucleotides 1295-1297, which resulted in the deduced loss of amino acid residue threonine at codon 332, and they displayed elevated free T4 levels and inappropriately normal TSH levels characteristic of other kindreds with GTHR. However, patient S1, who had two mutant alleles, had markedly elevated TSH and free T4 levels and displayed profound abnormalities in brain development and linear growth. A fibroblast c-erbA-beta cDNA extending from codon 175 to stop codon 457 was cloned from patient S1, sequenced, and used to create a full-length mutant cDNA. The kindred S mutant receptor was synthesized in vitro and did not bind T3. This mutant receptor did bind with similar avidity as the wild-type human beta-receptor to thyroid hormone response elements of the human TSH-beta (-12 to 43 bp) and rat GH (-188 to -160 bp) genes. Kindred S showed the effect in man of heterozygous and homozygous expression of a dominant negative form of c-erbA-beta. C1 CASE WESTERN RESERVE UNIV,SCH MED,DEPT MED,CLEVELAND,OH 44106. NIDDKD,BETHESDA,MD 20892. INST CANC MONTREAL,MONTREAL H2L 4M1,QUEBEC,CANADA. UNIV S FLORIDA,COLL MED,DEPT PEDIAT,TAMPA,FL 33612. RP USALA, SJ (reprint author), E CAROLINA UNIV,SCH MED,DEPT MED,GREENVILLE,NC 27858, USA. FU NIDDK NIH HHS [1R29-DK-42807-01] NR 43 TC 116 Z9 117 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD MAR PY 1991 VL 5 IS 3 BP 327 EP 335 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA FE674 UT WOS:A1991FE67400003 PM 1653889 ER PT J AU KALMAN, M GENTRY, DR CASHEL, M AF KALMAN, M GENTRY, DR CASHEL, M TI CHARACTERIZATION OF THE ESCHERICHIA-COLI K12 GLTS GLUTAMATE PERMEASE GENE SO MOLECULAR & GENERAL GENETICS LA English DT Article DE GLTS SEQUENCE; GLTS DELETION; BACTERIAL GLUTAMATE TRANSPORT ID SPOT GENE; TRANSPORT; CLONING; SEQUENCES; SUBUNIT; LOCUS; ACID AB The gltS gene is known to encode a sodiumdependent, glutamate-specific permease. We have localized the Escherichia coli K12 gltS gene with respect to the spoT gene, sequenced it, and recombined a null insertion-deletion allele into the chromosome without loss of viability. The gltS null allele gives a Glt- phenotype, i.e. it abolishes the ability of a gltC(c) host to grow on glutamate as sole carbon and nitrogen source and also confers alpha-methylglutamate resistance. A multicopy plasmid expressing the gltS gene can reverse the Glt- phenotype of gltS- or wild-type strains while other plasmids show host-dependent complementation patterns. Induction of gltS gene overexpression under control of isopropyl-beta-D-thiogalactoside (IPTG)-inducible promoters severely inhibits growth. The GltS protein is deduced to be a 42425 dalton hydrophobic protein with 2 sets of 5 possible integral protein domains, each flanking a central hydrophilic, flexible region. C1 NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. UNIV WISCONSIN,MCARDLE LAB CANC RES,MADISON,WI 53706. NR 25 TC 32 Z9 34 U1 1 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0026-8925 J9 MOL GEN GENET JI Mol. Gen. Genet. PD MAR PY 1991 VL 225 IS 3 BP 379 EP 386 PG 8 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA FE231 UT WOS:A1991FE23100005 PM 2017136 ER PT J AU BERZOFSKY, JA AF BERZOFSKY, JA TI MECHANISMS OF T-CELL RECOGNITION WITH APPLICATION TO VACCINE DESIGN SO MOLECULAR IMMUNOLOGY LA English DT Article; Proceedings Paper CT VACCINE SYMP CELEBRATING THE 75TH ANNIVERSARY OF CONNAUGHT LABORATORIES CY OCT, 1989 CL UNIV TORONTO, TORONTO, CANADA SP CONNAUGHT LABS HO UNIV TORONTO ID TOXIC LYMPHOCYTES-T; MALARIA CIRCUMSPOROZOITE PROTEIN; ENVELOPE PROTEIN; PLASMODIUM-FALCIPARUM; IMMUNOGENIC PEPTIDES; ANTIGEN PRESENTATION; SYNTHETIC IMMUNOGEN; VIRUS-REPLICATION; EPITOPES; SITES AB Both helper and cytotoxic T lymphocytes generally recognize protein antigens not in their intact form, as antibodies do, but on the surface of another cell, after "processing" by that cell to unfold or cleave the protein into fragments and after association of the processed antigen with major histocompatibility complex (MHC) molecules on that cell. This complex process leads to immunodominance of certain segments from the protein, which depends not only on structural features intrinsic to the antigenic segment itself, but also on antigen processing and on the structure of the MHC molecules of the responding individual. We have explored all three of these factors, including the enzymes involved in processing, the way peptides bind to MHC molecules, and structural features such as helical amphipathicity that seem to favour T cell recognition. We have used this information to locate and characterize antigenic sites of proteins of interest for vaccine development, including proteins from the malaria parasite and the AIDS virus, HIV. For HIV, we have identified both helper and cytotoxic T cell sites, coupled a helper site to a B cell site to produce a synthetic immunogen that elicits neutralizing antibodies, and studied the effect of viral sequence variation on cytotoxic T cell recognition and binding of the immunodominant peptide to MHC molecules. This information suggests strategies for the rational design of synthetic or recombinant vaccines. RP BERZOFSKY, JA (reprint author), NCI,MOLEC IMMUNOGENET & VACCINE RES SECT,BETHESDA,MD 20892, USA. NR 59 TC 18 Z9 18 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0161-5890 J9 MOL IMMUNOL JI Mol. Immunol. PD MAR PY 1991 VL 28 IS 3 BP 217 EP 223 DI 10.1016/0161-5890(91)90064-Q PG 7 WC Biochemistry & Molecular Biology; Immunology SC Biochemistry & Molecular Biology; Immunology GA FF843 UT WOS:A1991FF84300004 PM 1708102 ER PT J AU LIN, FH CLARK, G BIRNBAUM, LS LUCIER, GW GOLDSTEIN, JA AF LIN, FH CLARK, G BIRNBAUM, LS LUCIER, GW GOLDSTEIN, JA TI INFLUENCE OF THE AH LOCUS ON THE EFFECTS OF 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN ON THE HEPATIC EPIDERMAL GROWTH-FACTOR RECEPTOR SO MOLECULAR PHARMACOLOGY LA English DT Article ID PROTEIN KINASE-C; PLASMA-MEMBRANE; PHORBOL ESTERS; FACTOR-ALPHA; GUINEA-PIG; BINDING; RAT; MOUSE; INTERNALIZATION; MECHANISM AB The present studies examine whether the Ah receptor mediates the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the binding capacity of the hepatic epidermal growth factor (EGF) receptor in congenic strains of C57BL/6J mice that differ only at the Ah locus. The Ah locus is believed to encode the Ah receptor, which mediates the induction of cytochrome P45011A1 by TCDD and appears to mediate many of the toxic effects of TCDD. TCDD produced an 80-90% decrease in the maximum binding capacity (both high and low affinity sites) of the hepatic EGF receptor in female Ah-responsive (Ah(b/b) and Ah-nonresponsive (Ah(d/d) C57BL/6 mice. However, the ED50 for the effects of TCDD on the binding capacity of the EGF receptor was 10-fold higher in the Ah-nonresponsive mice, compared with the Ah-responsive mice (7 versus 0.7-mu-g/kg). TCDD did not affect the hepatic content of two EGF receptor mRNA transcripts (10 and 6 kb), indicating that the effects on the EGF receptor are not pretranslational. Similarly, TCDD did not affect the hepatic content of mRNA for transforming growth factor-alpha, an alternate ligand for the EGF receptor that is synthesized in the liver. In contrast, TCDD markedly increased the hepatic content of the mRNA for cytochrome P4501A1, which is known to be regulated transcriptionally by TCDD. The ED50 for this effect was 10-fold higher in Ah-nonresponsive mice than in Ah-responsive mice (13 versus 1.3-mu-g/kg). This study indicates that the effects of TCDD on EGF receptor ligand binding are mediated by the Ah receptor. However, unlike the effect of TCDD on cytochrome P4501A1, the effects of TCDD on the EGF receptor do not involve changes in the levels of the mRNA for this protein or changes in the mRNA for transforming growth factor-alpha, an alternate ligand for the EGF receptor. C1 NIEHS,BIOCHEM RISK ANAL LAB,B3-02,POB 12233,RES TRIANGLE PK,NC 27709. NIEHS,SYST TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. NR 40 TC 57 Z9 58 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD MAR PY 1991 VL 39 IS 3 BP 307 EP 313 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FD156 UT WOS:A1991FD15600007 PM 1848654 ER PT J AU BOJA, JW RAHMAN, MA PHILIP, A LEWIN, AH CARROLL, FI KUHAR, MJ AF BOJA, JW RAHMAN, MA PHILIP, A LEWIN, AH CARROLL, FI KUHAR, MJ TI ISOTHIOCYANATE DERIVATIVES OF COCAINE - IRREVERSIBLE INHIBITION OF LIGAND-BINDING AT THE DOPAMINE TRANSPORTER SO MOLECULAR PHARMACOLOGY LA English DT Article ID H-3 COCAINE; UPTAKE SITES; THREO-(+/-)-METHYLPHENIDATE BINDING; GBR-12935 BINDING; ACETYLCHOLINE-RECEPTOR; STRIATAL MEMBRANES; NONHUMAN-PRIMATES; UPTAKE COMPLEX; METAPHIT; PHENCYCLIDINE AB Isothiocyanate derivatives of (-)-cocaine were prepared and tested for inhibitory potency at the cocaine receptor in rat striatal membranes. Coincubation with m-isothiocyanatobenzoylecgonine methyl ester (m-ISOCOC), p-isothiocyanatobenzoylecgonine methyl ester (p-ISOCOC), and 3-beta-(4-isothiocyanatophenyl)tropane-2-carboxylic acid methyl ester (ISOWIN) resulted in inhibition of [H-3]WIN 35,428 binding, but the compounds were about 10-fold weaker than (-)-cocaine. However, p-ISOCOC was approximately 3-fold more potent than metaphit, an isothiocyanate derivative of phencyclidine. p-ISOCOC was equipotent at the serotonin transporter but was much less potent at the norepinephrine transporter and was inactive at the D2 dopamine receptor at 1000-mu-m concentration. The IC50 value for m-ISOCOC and p-ISOCOC varied with tissue concentration, suggesting irreversible inhibition of binding. Preincubation with m-ISOCOC and p-ISOCOC resulted in inhibition of [H-3]WIN 35,428 binding that could not be removed by washing of the membranes; in contrast, preincubation with (-)-cocaine caused inhibition that was readily removed by washing. Preincubation with 1-mu-M concentrations of p-ISOCOC resulted in a large reduction in B(max) of the high affinity binding site for [H-3]WIN 35,428. Preincubation with 100-mu-M p-ISOCOC eliminated the high affinity site and apparently reduced the affinity at the low affinity site. Coincubation of 10-mu-M p-ISOCOC with 100-mu-M cocaine prevented the total loss of [H-3]WIN 35,428 binding. The uptake of [H-3]dopamine was inhibited by p-ISOCOC with an IC50 comparable to that of cocaine. Additionally, preincubation of rat striatal synaptosomes with 10-mu-M p-ISOCOC reduced the V(max) of [H-3]dopamine uptake after washing. These data suggest that m-ISOCOC and p-ISOCOC are useful irreversible acylators of (-)-cocaine binding sites at the dopamine transporter. C1 NIDA,ADDICT RES CTR,NEUROSCI BRANCH,POB 5180,BALTIMORE,MD 21224. RES TRIANGLE INST,RES TRIANGLE PK,NC 27709. FU NIDA NIH HHS [DA05477] NR 37 TC 46 Z9 46 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD MAR PY 1991 VL 39 IS 3 BP 339 EP 345 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FD156 UT WOS:A1991FD15600011 PM 1826041 ER PT J AU KANTERMAN, RY MAHAN, LC BRILEY, EM MONSMA, FJ SIBLEY, DR AXELROD, J FELDER, CC AF KANTERMAN, RY MAHAN, LC BRILEY, EM MONSMA, FJ SIBLEY, DR AXELROD, J FELDER, CC TI TRANSFECTED D2 DOPAMINE-RECEPTORS MEDIATE THE POTENTIATION OF ARACHIDONIC-ACID RELEASE IN CHINESE-HAMSTER OVARY CELLS SO MOLECULAR PHARMACOLOGY LA English DT Article ID PROTEIN KINASE-C; ANTERIOR-PITUITARY; SIGNAL TRANSDUCTION; BINDING-PROTEINS; MAMMALIAN-CELLS; PHOSPHOLIPASE-A2; STIMULATION; ACTIVATION; IDENTIFICATION; DERIVATIVES AB A rat D2L dopamine receptor, a splice variant of the D2 receptor, has recently been cloned. When transfected into and stably expressed in Chinese hamster ovary cells, these receptors mediate the inhibition of both basal and forskolin-stimulated cAMP production, as previously described. We examined what role this receptor might play in the production of the second messenger arachidonic acid. The calcium ionophore A23187 stimulated the release of arachidonic acid, and this release of arachidonic acid was potentiated by dopamine in a concentration-dependent manner. Dopamine alone, however, had no effect on arachidonic acid release. Quinpirole, a D2-selective agonist, augmented A23187-stimulated arachidonic acid release, and sulpiride, a D2-selective antagonist, blocked this augmentation. cAMP analogs and agents that activate adenylyl cyclase were utilized in an attempt to overcome this dopamine effect. Forskolin, prostaglandin E2, dibutyryl-cAMP, 8-(4-chlorophenylthio)-cAMP, and pertussis toxin all had no appreciable effect on either A23187-stimulated arachidonic acid release or the dopamine enhancement. Inhibition of protein kinase C using long term phorbol ester desensitization and pharmacological inhibitors diminished the dopamine potentiation of arachidonic acid release. These results suggest that the D2 receptor may be increasing the release of arachidonic acid by a mechanism involving protein kinase C but independent of the D2 receptor's inhibition of adenylyl cyclase. C1 NIMH,CELL BIOL LAB,BLDG 36,ROOM 3A-15,BETHESDA,MD 20892. NIH,HOWARD HUGHES MED INST,RES SCHOLARS PROGRAM,BETHESDA,MD 20814. NINCDS,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NR 32 TC 127 Z9 128 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD MAR PY 1991 VL 39 IS 3 BP 364 EP 369 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FD156 UT WOS:A1991FD15600015 PM 1848657 ER PT J AU LIJINSKY, W AF LIJINSKY, W TI THE FORMATION AND OCCURRENCE OF POLYNUCLEAR AROMATIC-HYDROCARBONS ASSOCIATED WITH FOOD SO MUTATION RESEARCH LA English DT Review DE POLYNUCLEAR AROMATIC HYDROCARBONS; BENZO[A]PYRENE; CHARCOAL BROILED MEAT; CARCINOGENESIS ID CARCINOGENICITY; SMOKE AB Polynuclear aromatic hydrocarbons are common contaminants of processed food, usually at trace levels. These hydrocarbons are products of combustion and pyrolysis, and are present in petroleum and coal, and in products derived from them. Most polynuclear aromatic hydrocarbons are not carcinogenic, but some of them are, and a few are potent inducers of skin and lung tumors in mice. Their carcinogenic properties have not been fully explored, but they seem to be less potent by ingestion or inhalation, and they are known as a group to produce cancer in humans. The most effective carcinogens among them are those with 5 or 6 fused rings, and these tend to be less prevalent in mixtures than the 3- and 4-ring hydrocarbons, most of which are not carcinogenic. Sophisticated analytical methods, using solvent extraction and chromatography have been developed to detect and measure polynuclear aromatic hydrocarbons at levels of 1 in 10(9) (1 part per billion) or less, and these have been applied to the measurement of individual compounds in foods, as well as in products of combustion and pyrolysis. Wood smoke and smoked foods contain the carcinogenic benzo[a]pyrene at levels of 1 ppb, and other hydrocarbons; liquid smoke has lower levels. Crude vegetable oils have higher concentrations, but purified 'deodorized' oils have benzo[a]pyrene levels near 1 ppb. Sausages cooked over burning logs had as much as 200 ppb benzo[a]pyrene. Charcoal-broiled steaks and ground meat had benzo[a]pyrene concentrations up to 50-mu-g/kg, while less fatty pork and chicken had lower concentrations (up to 10-mu-g/kg). It was probable that the rendered fat dripped on to the hot charcoal and pyrolyzed to form quantities of polynuclear aromatic hydrocarbons, which rose with the smoke to deposit on the meat. Therefore, oven cooking or cooking with a heat source above the meat, or segregation of the meat from the smoke resulted in food containing negligible amounts of polynuclear aromatic hydrocarbons. Modifications of cooking practices accordingly would greatly reduce exposure to this group of carcinogens. RP LIJINSKY, W (reprint author), NCI,FREDERICK CANC RES FACIL,BRI BASIC RES PROGRAM,FREDERICK,MD 21701, USA. FU NCI NIH HHS [N01-CO-74101] NR 45 TC 141 Z9 146 U1 2 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD MAR-APR PY 1991 VL 259 IS 3-4 BP 251 EP 261 DI 10.1016/0165-1218(91)90121-2 PG 11 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA FH845 UT WOS:A1991FH84500005 PM 2017211 ER PT J AU DIPPLE, A BIGGER, CAH AF DIPPLE, A BIGGER, CAH TI MECHANISM OF ACTION OF FOOD-ASSOCIATED POLYCYCLIC AROMATIC HYDROCARBON CARCINOGENS SO MUTATION RESEARCH LA English DT Review DE POLYCYCLIC AROMATIC HYDROCARBONS; MECHANISM OF MUTAGENESIS AND CARCINOGENESIS; MUTAGENESIS, MECHANISM OF; CARCINOGENESIS, MECHANISM OF ID EMBRYO CELL-CULTURES; MOUSE SKIN; DNA ADDUCTS; CHEMICAL CARCINOGENESIS; DEOXYRIBONUCLEIC ACID; SHUTTLE VECTOR; DIOL-EPOXIDES; METABOLIC-ACTIVATION; NUCLEOSIDE ADDUCTS; ARALKYLATION AB The polycyclic aromatic hydrocarbon carcinogens are formed in the inefficient combustion of organic matter and contaminate foods through direct deposition from the atmosphere or during cooking or smoking of foods. These potent carcinogens and mutagens require metabolism to dihydrodiol epoxide metabolites in order to express their biological activities. In vitro studies show that these reactive metabolites can react with the bases in DNA with different specificities depending upon the hydrocarbon from which they are derived. Thus, the more potent carcinogens react more extensively with adenine residues in DNA than do the less potent carcinogens, with the result that mutation at A.T base pairs is enhanced for the more potent carcinogens. In the past few years, considerable clarification of the mechanisms of metabolic activation have been achieved and the focus for the immediate future is expected to be on how the reactive metabolites actually bring about biological responses. RP DIPPLE, A (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ABI BASIC RES PROGRAM,POB B,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74101] NR 73 TC 29 Z9 30 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD MAR-APR PY 1991 VL 259 IS 3-4 BP 263 EP 276 DI 10.1016/0165-1218(91)90122-3 PG 14 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA FH845 UT WOS:A1991FH84500006 PM 2017212 ER PT J AU POVEY, AC SCHIFFMAN, M TAFFE, BG HARRIS, CC AF POVEY, AC SCHIFFMAN, M TAFFE, BG HARRIS, CC TI LABORATORY AND EPIDEMIOLOGIC STUDIES OF FECAPENTAENES SO MUTATION RESEARCH LA English DT Review DE FECAPENTAENES ID FECAL MUTAGENS; COLON CANCER; HUMAN FECES; HUMAN-CELLS; DNA DAMAGE; GENOTOXICITY; FIBROBLASTS; FORMALDEHYDE; POPULATIONS; CARCINOGEN AB Fecapentaenes are a class of conjugated ether lipids which have been identified as the major component of human fecal mutagenicity in the Ames Salmonella mutagenesis assay. Human epidemiologic data have indicated that most healthy North American populations eating a western diet do excrete detectable levels of fecapentaenes. Excreted fecapentaene levels seem to reflect levels throughout the colonic lumen, and levels vary characteristically between individuals. Those individuals found to excrete high levels of fecapentaene appear, based on limited data, to be at decreased risk of colorectal neoplasia. Carcinogenicity studies in rats and mice have been predominantly negative, however, increased tumor incidence in mice exposed to fecapentaenes as neonates has recently been reported. Fecapentaenes are direct-acting genotoxins, which may react with DNA through free radical mechanisms, and/or aldehyde formation. Mechanisms by which fecapentaene-induced DNA damage may mediate carcinogenesis are discussed. C1 NCI,HUMAN CARCINOGENESIS LAB,BLDG 37,ROOM 2C05,BETHESDA,MD 20892. CHRISTIE HOSP & HOLT RADIUM INST,DEPT CARCINOGENESIS,MANCHESTER M20 9BX,LANCS,ENGLAND. NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. NR 52 TC 19 Z9 19 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD MAR-APR PY 1991 VL 259 IS 3-4 BP 387 EP 397 DI 10.1016/0165-1218(91)90129-A PG 11 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA FH845 UT WOS:A1991FH84500013 PM 2017218 ER PT J AU POMMIER, Y RUNGER, TM KERRIGAN, D KRAEMER, KH AF POMMIER, Y RUNGER, TM KERRIGAN, D KRAEMER, KH TI RELATIONSHIP OF DNA STRAND BREAKAGE PRODUCED BY BROMODEOXYURIDINE TO TOPOISOMERASE-II ACTIVITY IN BLOOM-SYNDROME FIBROBLASTS SO MUTATION RESEARCH LA English DT Article DE BLOOM-SYNDROME FIBROBLASTS; DNA STRAND BREAKAGE; BROMODEOXYURIDINE; TOPOISOMERASE-II ID SISTER-CHROMATID EXCHANGES; SYNDROME CELLS; LIGASE-I; INCREASE AB Cells from patients with Bloom syndrome, a cancer-prone disorder with cutaneous photosensitivity and spontaneous chromosome breakage, exhibit an abnormally increased number of sister-chromatid exchanges following treatment with 5-bromodeoxyuridine (BrdU). This effect has been postulated to be mediated by abnormal topoisomerase II activity. We used alkaline elution to measure DNA single-strand breakage following prolonged exposure to BrdU. Five-day exposure to BrdU produced equal numbers of alkali-labile sites in normal and Bloom-syndrome fibroblasts. These breaks were not protein-associated but were produced by alkali. Treatment with topoisomerase II inhibitors induced similar frequencies of DNA single-strand breaks in normal and Bloom-syndrome fibroblasts. These findings imply that BrdU incorporation into cellular DNA induces alkali-labile DNA lesions that are independent of topoisomerase II activity in Bloom and normal cells. C1 NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. RP POMMIER, Y (reprint author), NCI,MOLEC PHARMACOL LAB,BLDG 37,RM 5C27,BETHESDA,MD 20892, USA. FU Intramural NIH HHS [Z01 BC004517-31] NR 17 TC 5 Z9 5 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD MAR PY 1991 VL 254 IS 2 BP 185 EP 190 DI 10.1016/0921-8777(91)90010-M PG 6 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA FB445 UT WOS:A1991FB44500010 PM 1848352 ER PT J AU GONZALEZ, FJ CRESPI, CL GELBOIN, HV AF GONZALEZ, FJ CRESPI, CL GELBOIN, HV TI CDNA-EXPRESSED HUMAN CYTOCHROME-P450S - A NEW AGE OF MOLECULAR TOXICOLOGY AND HUMAN RISK ASSESSMENT SO MUTATION RESEARCH LA English DT Review DE CYTOCHROME-P450; CDNA EXPRESSION VECTORS; MUTAGEN TESTING SYSTEMS ID HUMAN-LIVER CYTOCHROME-P-450; OXIDATIVE DRUG-METABOLISM; AMINO-ACID SEQUENCE; RAT HEPATIC CYTOCHROME-P-450; DIRECTED EXPRESSION; COMPLEMENTARY-DNA; MESSENGER-RNA; GENETIC-POLYMORPHISM; TESTOSTERONE 6-BETA-HYDROXYLASE; MEPHENYTOIN 4-HYDROXYLASE AB It has long been recognized that a large degree of species differences exists among drug and carcinogen metabolizing enzymes. In particular, differences in cytochrome P450s, the principal enzymes of metabolic activation of procarcinogens, are widespread and may determine species and individual susceptibility to cancer causing chemicals. Although species differences in both the regulation and catalytic activities of P450s are quite large, rodent-based systems are mainly used as the means to determine the degree of hazard of environmental pollutants, pesticides, drugs and other environmental chemicals to humans. During recent years, a large effort has been expended on analyzing directly the structure, properties and catalytic activities of P450s from human tissues. In vitro mutagen testing systems, based on activation by human P450s, are being developed that will supplement other test systems in order to more accurately predict human risk to chemical exposure. C1 GENTEST CORP,WOBURN,MA 01801. RP GONZALEZ, FJ (reprint author), NCI,MOLEC CARCINOGENESIS LAB,BLDG 37,ROOM 3E-24,BETHESDA,MD 20892, USA. NR 110 TC 159 Z9 161 U1 0 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD MAR PY 1991 VL 247 IS 1 BP 113 EP 127 DI 10.1016/0027-5107(91)90039-Q PG 15 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA FB226 UT WOS:A1991FB22600013 PM 2002797 ER PT J AU BACHEVALIER, J LANDIS, LS WALKER, LC BRICKSON, M MISHKIN, M PRICE, DL CORK, LC AF BACHEVALIER, J LANDIS, LS WALKER, LC BRICKSON, M MISHKIN, M PRICE, DL CORK, LC TI AGED MONKEYS EXHIBIT BEHAVIORAL DEFICITS INDICATIVE OF WIDESPREAD CEREBRAL-DYSFUNCTION SO NEUROBIOLOGY OF AGING LA English DT Article DE MACACA-MULATTA; DELAYED RESPONSE; OBJECT DISCRIMINATION LEARNING; MEMORY; VISUOSPATIAL ORIENTATION; VISUALLY GUIDED REACHING; MOTOR SKILL LEARNING; REACTION TIME ID RHESUS-MONKEY; PREFRONTAL CORTEX; MEMORY DYSFUNCTION; MACACA-MULATTA; RECOGNITION MEMORY; SENILE PLAQUES; OLDER ADULTS; LESIONS; DISCRIMINATION; LOCALIZATION AB To determine whether the decline of behavioral abilities with aging in monkeys is selective or widespread, we examined 18 monkeys ranging from 3 to 34 years of age on a wide variety of tests with the ultimate goal of correlating behavioral deficits with age-related changes in the brain. In our initial study we found impaired visual recognition ability in the aged monkeys (43). In the present study, we assessed the same animals on tests of spatial memory, visual habit formation, visuospatial orientation, visually gudied reaching, motor skill learning, and reaction time, these categories having been chosen to test the integrity of different cerebral systems. There were three major findings. First, age-related impairments were observed in nearly all test categories, though often not on easy versions of the tests, suggesting that the deficits observed were in the specific abilities measured and not an artifact of lowered motivation or other general disability. Second, the behavioral decline began in the late teens for certain spatial abilities but did not affect other abilities until the late 20's, suggesting that although the cerebral dysfunction eventually becomes widespread, the cerebral systems underlying spatial abilities are compromised by aging earlier than others. Finally, the finding of correlations between scores of aged animals primarily within tests categories as opposed to across categories suggests that different animals have different patterns of cerebral involvement. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT PATHOL,NEUROPATHOL LAB,509 PATHOL BLDG,600 N WOLFE ST,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DIV COMPARAT MED,BALTIMORE,MD 21205. NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. RI Walker, L/J-6541-2015 OI Walker, L/0000-0001-9166-3261 FU NIA NIH HHS [AG 05146, AG 07914]; NINDS NIH HHS [NS 20471] NR 57 TC 185 Z9 188 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0197-4580 J9 NEUROBIOL AGING JI Neurobiol. Aging PD MAR-APR PY 1991 VL 12 IS 2 BP 99 EP 111 DI 10.1016/0197-4580(91)90048-O PG 13 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA FA963 UT WOS:A1991FA96300003 PM 2052134 ER PT J AU FREO, U RAPOPORT, SI SONCRANT, TT AF FREO, U RAPOPORT, SI SONCRANT, TT TI AGE-RELATED ALTERATIONS IN BEHAVIORAL AND CEREBRAL METABOLIC RESPONSES TO THE SEROTONIN AGONIST META-CHLOROPHENYLPIPERAZINE IN RATS SO NEUROBIOLOGY OF AGING LA English DT Article DE DEOXYGLUCOSE; AGING; META-CHLOROPHENYLPIPERAZINE (MCPP); SEROTONIN-1B RECEPTORS; CEREBRAL METABOLISM; RAT ID 5-HT1B RECEPTORS; BRAIN-REGIONS; SYSTEMS; 1-(M-TRIFLUOROMETHYLPHENYL)PIPERAZINE; INVOLVEMENT; ARECOLINE; RU-24969; DOPAMINE; BINDING; RELEASE AB To determine the functional relevance of the age-related neurochemical changes that occur in brain serotonin systems during aging, we measured the effects of the serotonin receptor agonist meta-chlorophenylpiperazine (MCPP) on behavior and on regional cerebral metabolic rates for glucose (rCMRglc) in awake rats. rCMRglc was determined in 74 regions of Fischer-344 rats aged 3, 12 and 24 months, at 15 and 90 min after MCPP 2.5 mg/kg IP, using the quantitative, autoradiographic [C-14]2-deoxy-D-glucose technique. The time-course of motor performance following MCPP was assessed with a rotating rod. MCPP impaired motor performance in all ages maximally at 15-30 min. Three-month-old rats recovered completely within 60 min, whereas 12-month-old animals exhibited partial recovery and 24-month-old rats did not recover by 120 min. At 15 min after MCPP, rCMRglc was reduced in 51 of the 74 studied regions (overall decrease, 20%) of 3-month-old rats, in 21 regions (13% decrease) of 12-month-old rats and in 14 regions (2% decrease) of 24-month-old animals. Similar MCPP brain concentrations were achieved at 15 min in rats of all ages. The results suggest that the functional integrity of serotonergic transmission is reduced in aged rats and that the dysregulation is presynaptic. C1 NIA,NEUROSCI LAB,BLDG 10,ROOM 6C103,BETHESDA,MD 20892. NR 47 TC 11 Z9 11 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0197-4580 J9 NEUROBIOL AGING JI Neurobiol. Aging PD MAR-APR PY 1991 VL 12 IS 2 BP 137 EP 144 PG 8 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA FA963 UT WOS:A1991FA96300007 PM 2052128 ER PT J AU WOOD, TL BERELOWITZ, M GELATO, MC ROBERTS, CT LEROITH, D MILLARD, WJ MCKELVY, JF AF WOOD, TL BERELOWITZ, M GELATO, MC ROBERTS, CT LEROITH, D MILLARD, WJ MCKELVY, JF TI HORMONAL-REGULATION OF RAT HYPOTHALAMIC NEUROPEPTIDE MESSENGER-RNAS - EFFECT OF HYPOPHYSECTOMY AND HORMONE REPLACEMENT ON GROWTH-HORMONE-RELEASING FACTOR, SOMATOSTATIN AND THE INSULIN-LIKE GROWTH-FACTORS SO NEUROENDOCRINOLOGY LA English DT Article DE INSULIN-LIKE GROWTH FACTOR-I; INSULIN-LIKE GROWTH FACTOR-II; GROWTH-HORMONE-RELEASING FACTOR; SOMATOSTATIN; GROWTH HORMONE; MESSENGER RNA REGULATION; HYPOTHALAMUS; RAT ID RIBONUCLEIC-ACID LEVELS; CENTRAL NERVOUS-SYSTEM; ANTERIOR-PITUITARY CELLS; FACTOR-I ACTION; SOMATOMEDIN-C; REGIONAL DISTRIBUTION; QUANTITATIVE AUTORADIOGRAPHY; RECEPTOR IMMUNOREACTIVITY; PERIVENTRICULAR NUCLEUS; DIFFERENTIAL EXPRESSION AB Hormonal feedback regulation of hypothalamic peptides putatively involved in growth hormone (GH) regulation has been studied by measurement of steady-state mRNA levels in male hypophysectomized rats with or without thyroid hormone, corticosterone, testosterone or GH replacement. Hypothalamic GH-releasing factor (GRF) mRNA levels increased progressively following hypophysectomy to 420% of sham levels after 15 days while hypothalamic insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF-II) mRNA levels decreased to less than 40% of sham levels. Whole hypothalamic somatostatin mRNA levels were not significantly different from sham. One week of continuous GH infusion restored hypothalamic IGF-I mRNA to levels (95%) indistinguishable from those in sham-operated controls but had no effect on either IGF-II or GRF mRNA. Thyroid hormone, corticosterone and testosterone treatment without GH had no effect on the hypophysectomy-induced reduction of either IGF-I or IGF-II mRNA levels but reversed the elevation of GRF mRNA. We conclude that hypothalamic IGF-I may be involved in GH feedback regulation and thus may function as a hypothalamic modulator of GH. In contrast, IGF-II may be regulated by one of the pituitary trophic hormones but not by GH or the target hormones tested. Finally, hypothalamic GRF mRNA regulation appears to be complex and may include target hormone feedback. C1 ABBOTT LABS,NEUROSCI VENTURE,D48E AP9,N CHICAGO,IL 60064. SUNY STONY BROOK,DEPT NEUROBIOL & BEHAV,STONY BROOK,NY 11794. SUNY STONY BROOK,DEPT INTERNAL MED,DIV ENDOCRINOL & METAB,STONY BROOK,NY 11794. NIDKD,DIABET BRANCH,BETHESDA,MD. UNIV FLORIDA,DEPT PHARMACODYNAM,GAINESVILLE,FL 32611. FU NICHD NIH HHS [HD22199]; NIMH NIH HHS [MH18010-03] NR 53 TC 51 Z9 51 U1 0 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0028-3835 J9 NEUROENDOCRINOLOGY JI Neuroendocrinology PD MAR PY 1991 VL 53 IS 3 BP 298 EP 305 DI 10.1159/000125732 PG 8 WC Endocrinology & Metabolism; Neurosciences SC Endocrinology & Metabolism; Neurosciences & Neurology GA FC973 UT WOS:A1991FC97300013 PM 1674982 ER PT J AU EHRLICH, GD GLASER, JB BRYZGORNIA, V MAESE, J WALDMANN, TA POIESZ, BJ GREENBERG, SJ AF EHRLICH, GD GLASER, JB BRYZGORNIA, V MAESE, J WALDMANN, TA POIESZ, BJ GREENBERG, SJ TI MULTIPLE-SCLEROSIS, RETROVIRUSES, AND PCR SO NEUROLOGY LA English DT Review ID VIRUS TYPE-I; TROPICAL SPASTIC PARAPARESIS; CELL LEUKEMIA LYMPHOMA; CHRONIC PROGRESSIVE MYELOPATHY; C POL-SEQUENCES; HTLV-I; ENZYMATIC AMPLIFICATION; LYMPHOTROPIC VIRUSES; NUCLEOTIDE-SEQUENCE; AIDS RETROVIRUS C1 SUNY HLTH SCI CTR,DEPT MED,DIV HEMATOL ONCOL,SYRACUSE,NY. STATEN ISL UNIV HOSP,DIV INFECT DIS,STATEN ISL,NY. NCI,METAB BRANCH,BETHESDA,MD 20892. FU NHLBI NIH HHS [N01 HB-67021, R01 HL-43602-01] NR 103 TC 46 Z9 46 U1 0 U2 2 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0028-3878 J9 NEUROLOGY JI Neurology PD MAR PY 1991 VL 41 IS 3 BP 335 EP 343 PG 9 WC Clinical Neurology SC Neurosciences & Neurology GA FD566 UT WOS:A1991FD56600001 PM 1848687 ER PT J AU FISHER, LJ JINNAH, HA KALE, LC HIGGINS, GA GAGE, FH AF FISHER, LJ JINNAH, HA KALE, LC HIGGINS, GA GAGE, FH TI SURVIVAL AND FUNCTION OF INTRASTRIATALLY GRAFTED PRIMARY FIBROBLASTS GENETICALLY MODIFIED TO PRODUCE L-DOPA SO NEURON LA English DT Article ID SUBSTANTIA NIGRA; MODIFIED CELLS; BRAIN GRAFTS; RAT STRIATUM; RETROVIRUS; DISEASE; GENE; EXPRESSION; LEVODOPA; SYSTEM AB A combination of gene transfer and intracerebral grafting may provide a powerful technique for examining the role of discrete substances in the development or functioning of the brain. In the present study, primary fibroblasts obtained from a skin biopsy from inbred Fischer rats were used as donor cells for genetic modification and grafting. When grafted to the striatum of Fischer rats with a prior 6-hydroxydopamine lesion, primary fibroblasts containing a transgene for either tyrosine hydroxylase (TH) or beta-galactosidase survived for 10 weeks and continued to express the transgene. TH synthesized by the implanted fibroblasts appeared to convert tyrosine to L-dopa actively, as observed in vitro, and to affect the host brain, as assessed through a behavioral measurement. These results suggest that primary fibroblasts genetically altered to express TH have the capacity to deliver L-dopa locally to the striatum in quantities sufficient to compensate partially for the loss of intrinsic striatal dopaminergic input. C1 NATL INST AGING,GERONTOL RES CTR,BIOL CHEM LAB,BALTIMORE,MD 21224. RP FISHER, LJ (reprint author), UNIV CALIF SAN DIEGO,DEPT NEUROSCI,LA JOLLA,CA 92093, USA. FU NCRR NIH HHS [S10 RR 04754-01]; NIA NIH HHS [AG08514] NR 37 TC 205 Z9 210 U1 0 U2 1 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0896-6273 J9 NEURON JI Neuron PD MAR PY 1991 VL 6 IS 3 BP 371 EP 380 DI 10.1016/0896-6273(91)90246-V PG 10 WC Neurosciences SC Neurosciences & Neurology GA FC470 UT WOS:A1991FC47000006 PM 1672072 ER PT J AU WADA, E WAY, J SHAPIRA, H KUSANO, K LEBACQVERHEYDEN, AM COY, D JENSEN, R BATTEY, J AF WADA, E WAY, J SHAPIRA, H KUSANO, K LEBACQVERHEYDEN, AM COY, D JENSEN, R BATTEY, J TI CDNA CLONING, CHARACTERIZATION, AND BRAIN REGION SPECIFIC EXPRESSION OF A NEUROMEDIN-B PREFERRING BOMBESIN RECEPTOR SO NEURON LA English DT Article ID SWISS 3T3 CELLS; RAT-BRAIN; BINDING-SITES; PEPTIDES; DNA; LOCALIZATION; SEQUENCE; SUBTYPES; PROTEIN AB Recent binding studies in the central nervous system and other tissues provide evidence that the mammalian bombesin-like peptides, gastrin-releasing peptide (GRP) and neuromedin-B (NMB), exert their numerous physiological effects through at least two different receptors. We describe the structure and expression of a cloned NMB-preferring bombesin receptor (NMB-R) with properties distinct from a GRP-preferring bombesin receptor (GRP-R) reported previously. In particular, the NMB-R shows higher affinity binding to NMB than to GRP in BALB 3T3 fibroblasts expressing the cloned NMB-R. The distinct regional distribution of NMB-R and GRP-R mRNA in the brain suggests that both bombesin receptor subtypes play independent roles in mediating many of the dramatic effects of bombesin-like peptides in the central nervous system. C1 CATHOLIC UNIV LOUVAIN,INST CELLULAR & MOLEC PATHOL,CELLULAR GENET UNIT,B-1200 BRUSSELS,BELGIUM. TULANE UNIV,MED CTR,DEPT MED,PEPTIDE RES LABS,NEW ORLEANS,LA 70112. NIDDKD,DIGEST DIS BRANCH,BETHESDA,MD 20892. RP WADA, E (reprint author), NINCDS,NEUROCHEM LAB,BETHESDA,MD 20892, USA. NR 34 TC 271 Z9 274 U1 0 U2 1 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0896-6273 J9 NEURON JI Neuron PD MAR PY 1991 VL 6 IS 3 BP 421 EP 430 DI 10.1016/0896-6273(91)90250-4 PG 10 WC Neurosciences SC Neurosciences & Neurology GA FC470 UT WOS:A1991FC47000010 PM 1848080 ER PT J AU MARLEY, RJ HENINGER, C HERNANDEZ, TD GALLAGER, DW AF MARLEY, RJ HENINGER, C HERNANDEZ, TD GALLAGER, DW TI CHRONIC ADMINISTRATION OF BETA-CARBOLINE-3-CARBOXYLIC ACID METHYLAMIDE BY CONTINUOUS INTRAVENTRICULAR INFUSION INCREASES GABAERGIC FUNCTION SO NEUROPHARMACOLOGY LA English DT Article DE BENZODIAZEPINE; GABA; FG-7142; CHRONIC DRUG ADMINISTRATION; INVERSE AGONIST; CHLORIDE UPTAKE ID STIMULATED CHLORIDE INFLUX; BENZODIAZEPINE RECEPTORS; ANTICONVULSANT TOLERANCE; CHRONIC DIAZEPAM; LIGAND; BRAIN; POTENTIATION; INHIBITORS; RESPONSES; CHANNELS AB The repeated, intraperitoneal administration of the benzodiazepine receptor inverse agonist, FG 7142 (beta-carboline-3-carboxylic acid methylamide), leads to pharmacological kindling and an associated decrease in GABA-stimulated influx of Cl-36- into cortical membrane preparations. The chronic administration of benzodiazepine agonists results in the development of tolerance and also results in a decrease in GABA-stimulated uptake of Cl-36-. The present study was designed to evaluate further the paradoxical reports that both chronic treatment with benzodiazepine receptor agonists and inverse agonists results in a decreased ability of GABA to stimulate uptake of Cl-36- into cortical membrane preparations. The effects of continuous administration of FG 7142 on GABA-stimulated uptake of Cl-36-, the threshold for bicuculline-induced seizures and the proconvulsant actions of acute administration FG 7142 were evaluated. The continuous administration of FG 7142 resulted in an increased capacity of GABA to stimulate the uptake of Cl-36- into cortical membrane preparations and a significant increase in the seizure threshold for bicuculline following the acute administration of FG 7142. These data, therefore, indicate that changes in GABAergic function following chronic administration of FG 7142 are dependent on the regimen of administration of drug. The results also suggest that the GABA receptor homeostatically responds to continuous occupation by inverse agonists by an upregulation of its functional response to GABA. C1 YALE UNIV,SCH MED,DEPT PSYCHIAT,ABRAHAM RIBICOFF RES FACIL,NEW HAVEN,CT 06508. CONNECTICUT MENTAL HLTH CTR,NEW HAVEN,CT 06508. RP MARLEY, RJ (reprint author), NIDA,ADDICT RES CTR,BALTIMORE,MD 21224, USA. FU NIMH NIH HHS [MH-13276]; NINDS NIH HHS [NS-23321] NR 31 TC 13 Z9 13 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0028-3908 J9 NEUROPHARMACOLOGY JI Neuropharmacology PD MAR PY 1991 VL 30 IS 3 BP 245 EP 251 DI 10.1016/0028-3908(91)90151-Z PG 7 WC Neurosciences; Pharmacology & Pharmacy SC Neurosciences & Neurology; Pharmacology & Pharmacy GA FC480 UT WOS:A1991FC48000005 PM 1649416 ER PT J AU GLOWA, JR AF GLOWA, JR TI DOSE-EFFECT APPROACHES TO RISK ASSESSMENT SO NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS LA English DT Article; Proceedings Paper CT SYMP ON ANIMAL-TO-HUMAN EXTRAPOLATION : ISSUES AND OPPORTUNITIES CY MAR 13-15, 1990 CL SAN ANTONIO, TX SP UNIV TEXAS SAN ANTONIO, COLL SCI & ENGN, DIV LIFE SCI, USAF, DIV HUMAN SYST, MED READINESS ADV DEV OFF, USAF, SCH AEROSP MED, DIV RADIAT SCI, SYST RES LAB, USN, MED RES & DEV COMMAND DE RISK ASSESSMENT; NEUROBEHAVIORAL TOXICOLOGY; TOLUENE ID VOLATILE ORGANIC-SOLVENTS; BEHAVIORAL-TOXICOLOGY; TOLUENE; MICE AB Risk assessment is the attempt to characterize the chance of obtaining an adverse effect after exposure to an agent. Traditionally, high levels of an agent have been used to estimate the likelihood a lower dose might have an effect either by using low-dose extrapolation models or by attempting to establish a dose with no observable effects (NOEL). Low-dose extrapolation models yield estimates for small effects, but these estimates may vary by orders of magnitude depending upon the function chosen to represent the data. NOEL's are imprecise because a true no-effect level is indeterminant and the inability to determine an observable effect depends primarily on background variability. Newer methods use data from portions of the dose-effect function where error is smaller to estimate risks. Risk estimates using two of these approaches are compared for two different sample sizes. Each method produced the same estimate with the larger sample at low risk, but with increasing levels of risk and smaller samples the estimates obtained using these methods diverged. RP GLOWA, JR (reprint author), NIMH,CLIN NEUROENDOCRINOL BRANCH,BIOPSYCHOL UNIT,BLDG 14D,ROOM 311,BETHESDA,MD 20892, USA. NR 27 TC 13 Z9 13 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0149-7634 J9 NEUROSCI BIOBEHAV R JI Neurosci. Biobehav. Rev. PD SPR PY 1991 VL 15 IS 1 BP 153 EP 158 DI 10.1016/S0149-7634(05)80108-3 PG 6 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA FC484 UT WOS:A1991FC48400026 PM 2052190 ER PT J AU STRONG, MJ WOLFF, AV WAKAYAMA, I GARRUTO, RM AF STRONG, MJ WOLFF, AV WAKAYAMA, I GARRUTO, RM TI ALUMINUM-INDUCED CHRONIC MYELOPATHY IN RABBITS SO NEUROTOXICOLOGY LA English DT Article DE ALUMINUM TOXICITY; CHRONIC MYELOPATHY; NEUROFILAMENT; PHOSPHORYLATION ID EXPERIMENTAL NEUROFIBRILLARY CHANGES; NEUROFILAMENT PHOSPHORYLATION; SYSTEMIC ALUMINUM; CHRONIC ANIMALS; MOTOR NEURONS; INTOXICATION; ANTIBODIES; PATHOLOGY; CHLORIDE; EXPOSURE AB Young adult New Zealand white rabbits, inoculated intracisternally once monthly with 100-mu-g AlCl3, developed progressive hyperreflexia, hypertonia, gait impairment, weight loss, muscle wasting and abnormal righting reflexes over the course of 8 months. No overt encephalopathic features were present. In spinal motor neuron perikarya, dendrites and axonal processes, argentophilic globular inclusions were extensive. Additionally, neurofibrillary tangle-like argentophilic inclusions were consistently present in the gigantocellularis, reticularis, raphe and trapezoid nuclei, but rarely present in the dorsal and ventral subiculum, parasubiculum and anterior thalamus, and never found in the cerebral cortex, substantia nigra, locus ceruleus, or cerebellum. All neuronal inclusions were immunoreactive with monoclonal antibodies recognizing phosphorylated and nonphosphorylated high and intermediate weight neurofilament proteins (SMl 31, SMl 32). Also, some spinal motor neuron inclusions were immunoreactive with a monoclonal antibody recognizing an 'age-related' phosphorylation state of neurofilament (SMl 34). Ultrastructurally, the inclusions consisted of straight or interwoven skeins of 10 nm filaments. This study demonstrates unique variability in the phosphorylation state of aluminum-induced neurofilamentous inclusions in a predominantly motor system degeneration induced by chronic low dose AlCl3. C1 NIH,CENT NERVOUS SYST STUDIES LAB,BETHESDA,MD 20892. RI Strong, Michael/H-9689-2012 NR 29 TC 59 Z9 61 U1 0 U2 1 PU INTOX PRESS INC PI LITTLE ROCK PA PO BOX 24865, LITTLE ROCK, AR 72221 SN 0161-813X J9 NEUROTOXICOLOGY JI Neurotoxicology PD SPR PY 1991 VL 12 IS 1 BP 9 EP 22 PG 14 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA EX215 UT WOS:A1991EX21500002 PM 1901636 ER PT J AU TANDON, P BARONE, S DRUST, EG TILSON, HA AF TANDON, P BARONE, S DRUST, EG TILSON, HA TI LONG-TERM BEHAVIORAL AND NEUROCHEMICAL EFFECTS OF INTRADENTATE ADMINISTRATION OF COLCHICINE IN RATS SO NEUROTOXICOLOGY LA English DT Article DE COLCHICINE; CARBACHOL; COMPENSATION; INOSITOLPHOSPHATE TURNOVER; HIPPOCAMPUS ID ENTORHINAL CORTEX LESIONS; HIPPOCAMPAL-FORMATION; PHOSPHOINOSITIDE HYDROLYSIS; DENTATE GYRUS; ADULT-RAT; CHOLINE-ACETYLTRANSFERASE; ALTERNATION PERFORMANCE; GRANULE CELLS; TIME COURSE; ACETYLCHOLINESTERASE AB Previous work in our laboratory has shown that the intradentate administration of colchicine produces time-dependent behavioral and neurochemical changes. Deficits in leaming and memory and alterations in the signal transduction process for the cholinergic muscarinic receptor have been observed up to 12 weeks after colchicine treatment. To study the long-term effects of colchicine administration on cognitive function and the cholinergic system, 6 month-old male, Fischer-344 rats were injected with 2.5-mu-g of colchicine bilaterally in the dorsal and ventral hippocampus. Twelve months later the animals were tested for the acquisition of a spatial reference memory task in the Morris water maze for 8 days, with 4 trials of 60 seconds each day. At the completion of the behavioral testing, one set of rats from each treatment group was used for histochemical studies. The remaining animals were sacrificed, the hippocampi removed and used for the estimation of receptor-stimulated turnover of phosphoinositides (Pl). [H-3]-Inositol was incorporated into the hippocampal slices, and various receptor agonists (carbachol, norepinephrine, serotonin) used to stimulate Pl turnover in the presence of lithium. A significant deficit in acquisition in the water maze was observed in animals 1 year after colchicine administration. Neurochemical studies showed an increase in carbachol-induced Pl metabolism in the rat hippocampus 1 year post-lesion with colchicine. However, in contrast to results obtained 12 weeks after lesioning, no significant changes were observed in norepinephrine or serotonin-induced Pl metabolism 1 year after lesioning. Pirenzepine, a M1 receptor antagonist, produced a greater degree of inhibition (62%) in lesioned animals as compared to the age-matched controls (20%). Increased staining for acetylcholinesterase was found in the hippocampus of treated rats. This effect is similar to that observed 12 weeks after lesioning. These data suggest that the effects of colchicine on the cholinergic system are long-lasting and can be observed a year after treatment. C1 NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,RES TRIANGLE PK,NC 27709. E CAROLINA UNIV,SCH MED,DEPT ANAT & CELL BIOL,GREENVILLE,NC 27858. NORWICH EATON PHARMACEUT INC,NORWICH,NY 13815. NR 41 TC 13 Z9 13 U1 0 U2 0 PU INTOX PRESS INC PI LITTLE ROCK PA PO BOX 24865, LITTLE ROCK, AR 72221 SN 0161-813X J9 NEUROTOXICOLOGY JI Neurotoxicology PD SPR PY 1991 VL 12 IS 1 BP 67 EP 78 PG 12 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA EX215 UT WOS:A1991EX21500007 PM 1849622 ER PT J AU YU, ML HSU, CC GLADEN, BC ROGAN, WJ AF YU, ML HSU, CC GLADEN, BC ROGAN, WJ TI INUTERO PCB/PCDF EXPOSURE - RELATION OF DEVELOPMENTAL DELAY TO DYSMORPHOLOGY AND DOSE SO NEUROTOXICOLOGY AND TERATOLOGY LA English DT Article DE CHILD DEVELOPMENT; POLYCHLOROBIPHENYL COMPOUNDS; POLYCHLORINATED DIBENZOFURANS; TERATOGENS ID POLYCHLORINATED-BIPHENYLS; TAIWAN; CHILDREN; PCBS; INFANTS; MOTHERS; BLOOD AB In 1979, there was an outbreak of food poisoning in central Taiwan due to cooking oil contaminated with polychlorinated biphenyls and their thermal degradation products. Starting in 1985, we studied 128 children born to exposed women after the oil was removed from the market; the exposure of these children was transplacental or through breast milk. We also studied matched controls. The exposed children exhibited developmental delays as measured by parental report, by neurologic examination, and by standard cognitive tests; delay was seen at all ages and persisted over time. Delay was greater in children who were smaller in size and in children who had exhibited symptoms of intoxication. Children with a history of nail deformity also were delayed. However, there was little relationship between other physical findings or measures of maternal exposure and developmental delay. There was some indication that the child's prenatal exposure was more important to developmental delay than was exposure through breast milk. C1 NIEHS,STAT & BIOMATH BRANCH,MAIL DROP B3-02,POB 12233,RES TRIANGLE PK,NC 27709. NIEHS,EPIDEMIOL BRANCH,RES TRIANGLE PK,NC 27709. NATL CHENG KUNG UNIV HOSP,DEPT PSYCHIAT,TAINAN,TAIWAN. RI Rogan, Walter/I-6034-2012 OI Rogan, Walter/0000-0002-9302-0160 NR 28 TC 56 Z9 57 U1 0 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0892-0362 J9 NEUROTOXICOL TERATOL JI Neurotoxicol. Teratol. PD MAR-APR PY 1991 VL 13 IS 2 BP 195 EP 202 DI 10.1016/0892-0362(91)90011-K PG 8 WC Neurosciences; Toxicology SC Neurosciences & Neurology; Toxicology GA FD442 UT WOS:A1991FD44200011 PM 1710764 ER PT J AU LEVINE, AS AF LEVINE, AS TI GOLD OUT OF LEAD - THE NIHS NEW FINANCIAL MANAGEMENT PLAN SO NEW BIOLOGIST LA English DT Editorial Material RP LEVINE, AS (reprint author), NIH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD MAR PY 1991 VL 3 IS 3 BP 199 EP 202 PG 4 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK718 UT WOS:A1991GK71800001 PM 1878348 ER PT J AU SUTRAVE, P HUGHES, SH AF SUTRAVE, P HUGHES, SH TI THE SKI ONCOGENE SO ONCOGENE LA English DT Review ID SEQUENCE; VIRUSES; MUSCLE; CELLS; GENE C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,POB B,FREDERICK,MD 21702. FU NCI NIH HHS [N01-CO-74101] NR 13 TC 24 Z9 24 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAR PY 1991 VL 6 IS 3 BP 353 EP 356 PG 4 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA FT344 UT WOS:A1991FT34400001 PM 2011394 ER PT J AU DOSAKAAKITA, H ROSENBERG, RK MINNA, JD BIRRER, MJ AF DOSAKAAKITA, H ROSENBERG, RK MINNA, JD BIRRER, MJ TI A COMPLEX PATTERN OF TRANSLATIONAL INITIATION AND PHOSPHORYLATION IN L-MYC PROTEINS SO ONCOGENE LA English DT Article ID CELL LUNG-CANCER; FIBROBLAST GROWTH-FACTOR; CASEIN KINASE-II; NON-AUG CODONS; C-MYC; MESSENGER-RNAS; EMBRYO FIBROBLASTS; GENE FAMILY; LINES; TRANSFORMATION AB Molecular analysis of the human proto-oncogene L-myc revealed a complex pattern of gene expression including alternative splicing and polyadenylation site selection of mRNA, giving rise to at least four mRNAs. These mRNAs in turn can code for several proteins. In this report, we characterize and define the origins of the major L-myc proteins. In vitro translation revealed that (i) two L-myc proteins (p59 and p65) were derived through alternative translational initiation at a non-AUG (CUG) site in intron 1 and at an AUC site in exon 2 of L-myc, and that (ii) extensive post-translational phosphorylation of these proteins yielded three additional proteins (p60, p66, and p68). Transfection experiments in rat embryo cells revealed the in vivo existence of this unusual CUG-initiated protein and demonstrated that it possessed transforming activity. Further, immuno-precipitation using high titered anti-L-myc peptide antisera, of two L-myc expressing small-cell lung cancer cell lines revealed three major L-myc proteins (p60, p66 and p68) all of which were derived from extensive phosphorylation of a p59 protein. C1 NCI,USN,MED ONCOL BRANCH,BETHESDA,MD 20892. UNIFORMED SERV UNIV HLTH SCI,BETHESDA,MD 20814. NR 40 TC 27 Z9 27 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAR PY 1991 VL 6 IS 3 BP 371 EP 378 PG 8 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA FT344 UT WOS:A1991FT34400004 PM 1849244 ER PT J AU PATTON, LL VALDEZ, IH AF PATTON, LL VALDEZ, IH TI XERODERMA-PIGMENTOSUM - REVIEW AND REPORT OF A CASE SO ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS LA English DT Article ID DNA-REPAIR; SKIN-CANCER; NEOPLASMS; CARCINOMA; TONGUE AB Xeroderma pigmentosum is a rare inherited dermatosis that provides insight into the basic mechanism of carcinogenesis. It is a model disorder linking defective DNA repair with clinical abnormalities and neoplasia. UV light-induced damage to the skin begins early and results in multiple benign and malignant skin tumors, especially in sun-exposed areas of the head and neck. Oral cancers, primarily squamous cell carcinomas of the anterior third of the tongue, occur with greatly increased frequency. A patient with multiple facial neoplasia and oral manifestations of xeroderma pigmentosum is presented. The role of the dentist in surveillance of oral and perioral structures is emphasized. The dentist is advised against the use of UV light-curing units in these patients because UV-induced epithelial damage may cause dysplasia when DNA repair mechanisms are dysfunctional. C1 NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892. RP PATTON, LL (reprint author), UNIV N CAROLINA,SCH DENT,DEPT DENT ECOL,CB7450,CHAPEL HILL,NC 27599, USA. OI Patton, Lauren/0000-0002-8253-4588 NR 24 TC 9 Z9 10 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 1079-2104 J9 ORAL SURG ORAL MED O JI Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. PD MAR PY 1991 VL 71 IS 3 BP 297 EP 300 DI 10.1016/0030-4220(91)90303-T PG 4 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA FB695 UT WOS:A1991FB69500009 PM 2011352 ER PT J AU ENGELKE, W TSUCHIMOCHI, M RUTTIMANN, UE HOSAIN, F AF ENGELKE, W TSUCHIMOCHI, M RUTTIMANN, UE HOSAIN, F TI ASSESSMENT OF BONE REMODELING IN THE TEMPOROMANDIBULAR-JOINT BY SERIAL UPTAKE MEASUREMENT OF TECHNETIUM 99M LABELED METHYLENE DIPHOSPHONATE WITH A CADMIUM TELLURIDE PROBE SO ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS LA English DT Article ID SCINTIGRAPHY AB Regional uptake of technetium 99m-labeled methylene diphosphonate was measured serially over time to follow the healing course of surgically generated lesions, induced unilaterally in the condyles of beagles. A small portable cadmium telluride probe was used for radiation detection, with and without a gold collimator attached. Radioactivity was measured at six periarticular sites on both the operated and the contralateral control sides and expressed as a ratio with respect to a reference site over the sagittal suture. The use of a collimator was crucial to separate the activity associated with bone mineralization in the condylar head from that arising in the adjoining bones. The uptake in the condylar region increased 2 weeks after surgery and remained at a constant level above the presurgery baseline (p < 0.05) until termination of the study (10 weeks). Postmortem histologic examinations confirmed the high bone turnover level by revealing newly formed bone as well as the presence of osteoclasts. The time course of nuclide uptake in the temporomandibular joint is different from the general pattern of rise and fall of activity displayed in other bones and indicates the persistence of bone remodeling processes during a prolonged period of at least 10 weeks. C1 NIDR,DIAGNOST SYST BRANCH,9000 ROCKVILLE PIKE,BLDG 10-IN104,BETHESDA,MD 20892. UNIV CONNECTICUT,CTR HLTH,DEPT NUCL MED,FARMINGTON,CT 06032. NR 18 TC 3 Z9 3 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 1079-2104 J9 ORAL SURG ORAL MED O JI Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. PD MAR PY 1991 VL 71 IS 3 BP 357 EP 363 DI 10.1016/0030-4220(91)90315-4 PG 7 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA FB695 UT WOS:A1991FB69500021 PM 2011362 ER PT J AU KOVACIK, V KOVAC, P GRUTZMACHER, HF AF KOVACIK, V KOVAC, P GRUTZMACHER, HF TI INFLUENCE OF FLUORO ATOMS ON THE SPONTANEOUS AND COLLISION-INDUCED FRAGMENTATION OF THE IONS [M-OCH3]+ AND [M+NH4]+ OF PER-O-ACETYLATED METHYL X-DEOXY-X-FLUORO-ALPHA-D-GLUCOPYRANOSIDES SO ORGANIC MASS SPECTROMETRY LA English DT Letter ID MASS-SPECTROMETRY C1 NIDKK,BETHESDA,MD 20892. UNIV BIELEFELD,FAK CHEM,W-4800 BIELEFELD,GERMANY. RP KOVACIK, V (reprint author), SLOVAK ACAD SCI,INST CHEM,CS-84238 BRATISLAVA,CZECHOSLOVAKIA. NR 7 TC 5 Z9 5 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0030-493X J9 ORG MASS SPECTROM JI Org. Mass Spectrom. PD MAR PY 1991 VL 26 IS 3 BP 171 EP 172 DI 10.1002/oms.1210260314 PG 2 WC Chemistry, Organic; Spectroscopy SC Chemistry; Spectroscopy GA FB066 UT WOS:A1991FB06600014 ER PT J AU DUBNER, R AF DUBNER, R TI PAIN AND HYPERALGESIA FOLLOWING TISSUE-INJURY - NEW MECHANISMS AND NEW TREATMENTS SO PAIN LA English DT Editorial Material ID DORSAL HORN NEURONS; RAT; RESPONSES RP DUBNER, R (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 30,ROOM B-18,BETHESDA,MD 20892, USA. NR 15 TC 67 Z9 70 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3959 J9 PAIN JI Pain PD MAR PY 1991 VL 44 IS 3 BP 213 EP 214 DI 10.1016/0304-3959(91)90087-E PG 2 WC Anesthesiology; Clinical Neurology; Neurosciences SC Anesthesiology; Neurosciences & Neurology GA FE405 UT WOS:A1991FE40500001 PM 1828877 ER PT J AU BROUWER, AE CARROLL, PB ATWATER, IJ AF BROUWER, AE CARROLL, PB ATWATER, IJ TI EFFECTS OF LEUCINE ON INSULIN-SECRETION AND BETA-CELL MEMBRANE-POTENTIAL IN MOUSE ISLETS OF LANGERHANS SO PANCREAS LA English DT Article DE PANCREATIC BETA-CELLS; INSULIN SECRETION; LEUCINE; MEMBRANE POTENTIAL; ISLETS OF LANGERHANS; MOUSE AB Leucine is known to enhance insulin secretion from islets of Langerhans, and insulin promotes leucine uptake in peripheral tissues. The present studies were designed to elucidate the effects of leucine on glucose responsiveness and stimulus secretion coupling in mouse islets of Langerhans. The effects of 20 mM leucine on insulin secretion and membrane potential were studied over a range of glucose concentrations (0-27.7 mM). Microdissected, perifused pancreatic islets from normal adult mice were used for both studies of insulin secretion and electrophysiology in order to make a close comparison between these measurements. Leucine enhanced the insulin secretion in the presence of 5.6, 11.1, and 22.2 mM glucose. In the presence of leucine, 27 mM glucose inhibited insulin secretion. In the absence of glucose-leucine did not induce electrical activity of the beta cell membrane, whereas in the presence of 5.6, 11.1, and 22.2 mM glucose leucine increased spike frequency. Thus, leucine shifts both the glucose-dependent insulin secretion and electrical activity toward lower glucose concentrations. It is concluded that leucine and glucose share a common metabolic pathway (citric acid cycle) for stimulatory effects. Leucine is deaminated to form 2-ketoisocaproic acid (KIC) and produce NH4+. We propose that in the absence of glucose this increases cytosolic pH, which in turn increases K+ permeability, and inhibits electrical activity and insulin secretion. C1 NIDDK,CELL BIOL & GENET LAB,BLDG 8,ROOM 326,BETHESDA,MD 20892. NR 0 TC 5 Z9 6 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0885-3177 J9 PANCREAS JI Pancreas PD MAR PY 1991 VL 6 IS 2 BP 221 EP 228 DI 10.1097/00006676-199103000-00015 PG 8 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA EY001 UT WOS:A1991EY00100015 PM 1886890 ER PT J AU ROSENBERG, ZF FAUCI, AS AF ROSENBERG, ZF FAUCI, AS TI IMMUNOPATHOLOGY AND PATHOGENESIS OF HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION SO PEDIATRIC INFECTIOUS DISEASE JOURNAL LA English DT Article DE HUMAN IMMUNODEFICIENCY VIRUS; IMMUNOPATHOLOGY ID IMMUNE-DEFICIENCY SYNDROME; TUMOR NECROSIS FACTOR; LYMPHADENOPATHY-ASSOCIATED VIRUS; PNEUMOCYSTIS-CARINII PNEUMONIA; AIDS DEMENTIA COMPLEX; B-CELL ACTIVATION; T-CELL; HTLV-III; HIV-1 INFECTION; MONONUCLEAR PHAGOCYTES RP ROSENBERG, ZF (reprint author), NIAID,BETHESDA,MD 20892, USA. NR 88 TC 11 Z9 12 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0891-3668 J9 PEDIATR INFECT DIS J JI Pediatr. Infect. Dis. J. PD MAR PY 1991 VL 10 IS 3 BP 230 EP 238 DI 10.1097/00006454-199103000-00012 PG 9 WC Immunology; Infectious Diseases; Pediatrics SC Immunology; Infectious Diseases; Pediatrics GA FC019 UT WOS:A1991FC01900012 PM 1674985 ER PT J AU KAVALIERS, M YANG, HYT AF KAVALIERS, M YANG, HYT TI EFFECTS OF MAMMALIAN FMRF-NH2-RELATED PEPTIDES AND IGG FROM ANTISERUM AGAINST THEM ON AGGRESSION AND DEFEAT-INDUCED ANALGESIA IN MICE SO PEPTIDES LA English DT Article DE FMRF-NH2-RELATED PEPTIDES; OPIOID ANTAGONISTS; OPIOID ANALGESIA; AGGRESSION; DEFEAT STRESS-INDUCED ANALGESIA ID FMRFAMIDE-LIKE IMMUNOREACTIVITY; CENTRAL NERVOUS-SYSTEM; RAT-BRAIN; MORPHINE; PHE-MET-ARG-PHE-NH2; NEUROPEPTIDE; INHIBITION; BUSPIRONE; NOCICEPTION; ANXIOLYTICS AB The effects of two endogenous mammalian FMRFamide (Phe-Met-Arg-Phe-NH2)-related peptides, an octapeptide F8Fa (Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2) and an octadecapeptide A18Fa(Ala-Gly-Glu-Gly-Leu-Ser-Ser-Pro-Phe-Trp-Ser-Leu-Ala-Pro-Gln-Arg-Phe-NH2), and IgG from serum against them on the responses to aggression and defeat-induced analgesia were examined in subordinate mice in "resident-intruder" pairings. Intracerebroventricular (ICV) administrations of F8Fa and A18Fa (0.01-10-mu-g) reduced, in a dose-dependent manner, the number of bites to obtain defeat in the subordinate mice during the agonistic encounters, as well as attenuating defeat-induced analgesia, with F8Fa having a greater inhibitory effect than A18Fa. Peripheral administration of naloxone (1.0 mg/kg) had a similar inhibitory effect on the number of bites to defeat and the level of defeat-induced analgesia. In contrast, ICV administrations of F8FaIgG and A18Fa-IgG antisera increased the number of bites to defeat and augmented the levels of defeat-induced analgesia, with F8Fa-IgG having a greater effect than A18Fa-IgG. These results provide further evidence that the peptides, F8Fa and A18Fa, are involved in the modulation of opioid-mediated analgesia accompanying biological stressors and suggest that these endogeneous FMRF-NH2-related peptides may also be associated with the expression of opioid-sensitive components of aggressive behavior. C1 UNIV WESTERN ONTARIO,DEPT PSYCHOL,LONDON N6A 5C1,ONTARIO,CANADA. ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,BIOCHEM GENET LAB,WASHINGTON,DC 20032. RP KAVALIERS, M (reprint author), UNIV WESTERN ONTARIO,FAC DENT,DIV ORAL BIOL,LONDON N6A 5C1,ONTARIO,CANADA. NR 40 TC 17 Z9 17 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PD MAR-APR PY 1991 VL 12 IS 2 BP 235 EP 239 DI 10.1016/0196-9781(91)90005-A PG 5 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA FE045 UT WOS:A1991FE04500005 PM 2067975 ER PT J AU ROTHMAN, RB MAHBOUBI, A BYKOV, V KIM, CH JACOBSON, AE RICE, KC AF ROTHMAN, RB MAHBOUBI, A BYKOV, V KIM, CH JACOBSON, AE RICE, KC TI PROBING THE OPIOID RECEPTOR COMPLEX WITH (+)-TRANS-SUPERFIT .1. EVIDENCE THAT [D-PEN2,D-PEN5]ENKEPHALIN INTERACTS WITH HIGH-AFFINITY AT THE DELTA-CX BINDING-SITE SO PEPTIDES LA English DT Article DE OPIOID RECEPTOR; ENKEPHALINS; RECEPTOR BINDING; AFFINITY LIGANDS; ANTINOCICEPTION; MORPHINE ID MU-MEDIATED ANTINOCICEPTION; MORPHINE-INDUCED ANALGESIA; 2-SITE ALLOSTERIC MODEL; RAT-BRAIN MEMBRANES; LEUCINE-ENKEPHALIN; OPIATE RECEPTORS; AGONISTS; MODULATION; INVITRO; INVIVO AB A variety of data support the existence of an opioid receptor complex composed of distinct but interacting mu-cx and delta-cx binding sites, where "cx" indicates "in the complex." The ability of subantinociceptive doses of [Leu5]enkephalin and [Met5]enkephalin to potentiate and attenuate morphine-induced antinociception, respectively, is thought to be mediated via their binding to the delta-cx binding site. [D-Pen2,D-Pen5]Enkephalin also modulates morphine-induced antinociception, but has very low affinity for the delta-cx binding site in vitro. In the present study, membranes were depleted of their delta-ncx binding sites by pretreatment with the site-directed acylating agent, (3S,4S)-(+)-trans-N-[1-[2-(4-isothiocyanato)phenyl)-ethyl]-3-methyl-4-piperidyl]-N-phenylpropaneamide hydrochloride, which permits selective labeling of the delta-cx binding site with [H-3][D-Ala2,D-Leu5]enkephalin. The major findings of this study are that with this preparation of rat brain membranes: a) there are striking differences between the delta-cx and mu binding sites; and b) both [D-Pen2,D-Pen5]enkephalin and [D-Pen2,L-Pen5]enkephalin exhibit high affinity for the delta-cx binding site. C1 NIDDK,MED CHEM LAB,DRUG DESIGN & SYNTHESIS SECT,BETHESDA,MD 20892. RP ROTHMAN, RB (reprint author), NIDDK,MED CHEM LAB,RECEPTOR STUDIES UNIT,BLDG 10 3D41,BETHESDA,MD 20892, USA. NR 32 TC 16 Z9 16 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PD MAR-APR PY 1991 VL 12 IS 2 BP 359 EP 364 DI 10.1016/0196-9781(91)90026-L PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA FE045 UT WOS:A1991FE04500026 PM 1648715 ER PT J AU CASSMAN, M AF CASSMAN, M TI THE EVOLUTION OF A SCIENCE ADVISORY BODY IN THE FEDERAL-GOVERNMENT SO PERSPECTIVES IN BIOLOGY AND MEDICINE LA English DT Article RP CASSMAN, M (reprint author), NIGMS,5333 WESTBARD AVE,ROOM 922,BETHESDA,MD 20892, USA. NR 26 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0031-5982 J9 PERSPECT BIOL MED JI Perspect. Biol. Med. PD SPR PY 1991 VL 34 IS 3 BP 439 EP 462 PG 24 WC History & Philosophy Of Science; Medicine, Research & Experimental SC History & Philosophy of Science; Research & Experimental Medicine GA FL098 UT WOS:A1991FL09800009 PM 2067936 ER PT J AU BASILE, AS JONES, EA SKOLNICK, P AF BASILE, AS JONES, EA SKOLNICK, P TI THE PATHOGENESIS AND TREATMENT OF HEPATIC-ENCEPHALOPATHY - EVIDENCE FOR THE INVOLVEMENT OF BENZODIAZEPINE RECEPTOR LIGANDS SO PHARMACOLOGICAL REVIEWS LA English DT Review ID GAMMA-AMINOBUTYRIC-ACID; PORTAL-SYSTEMIC ENCEPHALOPATHY; ANTAGONIST RO 15-1788; CHAIN AMINO-ACIDS; VISUAL EVOKED-POTENTIALS; BLOOD-BRAIN-BARRIER; CENTRAL NERVOUS-SYSTEM; ACUTE LIVER-FAILURE; CHRONIC PORTOSYSTEMIC ENCEPHALOPATHY; CEREBROSPINAL-FLUID GLUTAMINE C1 NIDDK,DIGEST DIS BRANCH,LIVER DIS SECT,BETHESDA,MD 20892. RP BASILE, AS (reprint author), NIDDK,NEUROSCI LAB,BLDG 8,ROOM 111,BETHESDA,MD 20892, USA. NR 494 TC 120 Z9 120 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0031-6997 J9 PHARMACOL REV JI Pharmacol. Rev. PD MAR PY 1991 VL 43 IS 1 BP 27 EP 71 PG 45 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FC876 UT WOS:A1991FC87600002 PM 1674609 ER PT J AU LIAU, LM SLEIGHT, AJ PITHA, J PEROUTKA, SJ AF LIAU, LM SLEIGHT, AJ PITHA, J PEROUTKA, SJ TI CHARACTERIZATION OF A NOVEL AND POTENT 5-HYDROXYTRYPTAMINE1A RECEPTOR ANTAGONIST SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Article DE 5-HT1A RECEPTORS; ADENYLATE CYCLASE; 5-HT BEHAVIORAL SYNDROME ID ADENYLATE-CYCLASE; SEROTONIN RECEPTORS; RAT HIPPOCAMPUS; HIGHLY POTENT; BRAIN; 5-HT1A; SITES; SPIROXATRINE; SUBTYPES; BINDING AB A series of pindolol derivatives (n = 7) was analyzed in radioligand binding, biochemical and behavioral studies. Three of these drugs (Compounds A, B, and C) are extremely potent (i.e., K(i) values < 1.0 nM) at 5-hydroxytryptamine1A (5-HT1A) sites labeled by [H-3] 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT). Moreover, these drugs are selective in that they are approximately an order of magnitude less potent at beta-adrenergic receptors labeled by H-3-dihydroalprenolol (DHA). Compound A (N1-(bromoacetyl)-N8-[3-(4-indolyloxy)-2-hydroxypropyl](Z)-1,8-diamino-p-methane) is also significantly less potent at 10 other neurotransmitter receptor sites analyzed. In addition, Compound A (10(-10) M to 10(-3) M) has no effect on baseline forskolin-stimulated adenylate cyclase activity in rat hippocampus. By contrast, nanomolar concentrations of the drug significantly (p < 0.01) reverse 8-OH-DPAT-induced inhibition of forskolin-stimulated activity. In behavioral studies, Compound A (0.5 mg/kg) alone has no effect on baseline measures of reciprocal forepaw treading in the rat. Pretreatment with Compound A, however, significantly (p < 0.05) inhibits the reciprocal forepaw treading induced by 8-OH-DPAT. These data suggest that Compound A is a potent and selective antagonist of 5-HT1A receptors in the CNS. C1 STANFORD UNIV,MED CTR,DEPT NEUROL,STANFORD,CA 94305. NIA,GRS,BALTIMORE,MD 21224. FU NINDS NIH HHS [NS 12151-14, NS 23560-03] NR 25 TC 43 Z9 43 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD MAR PY 1991 VL 38 IS 3 BP 555 EP 559 DI 10.1016/0091-3057(91)90013-R PG 5 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA FD888 UT WOS:A1991FD88800013 PM 1648748 ER PT J AU COX, M GARRICK, N REITE, M GENNARO, M AF COX, M GARRICK, N REITE, M GENNARO, M TI MINIPUMP CLORGYLINE ADMINISTRATION AND CSF AMINE METABOLITES IN UNRESTRAINED MONKEYS SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Note DE CLORGYLINE; MAOI; OSMOTIC MINIPUMP; MACACA-NEMESTRINA; MONKEYS; MHPG; 5-HIAA; HVA; CATECHOLAMINE; NONHUMAN PRIMATES; CEREBROSPINAL FLUID; CSF ID CEREBROSPINAL-FLUID; RHESUS-MONKEYS; CATECHOLAMINE; SEROTONIN; PARGYLINE; DOPAMINE; KETAMINE AB The irreversible MAO-A inhibitor clorgyline was administered in doses of 0.5 mg/kg (N = 1), 1 mg/kg (N = 3), and 2 mg/kg (N = 1) to 5 young (age 5.5 to 23.9 months) pigtail (M. nemestrina) monkeys using a 28-day (Alza 2ML4) osmotic minipump. CSF MHPG, 5-HIAA, HVA, and plasma MHPG were measured before and at approximately weekly intervals after pump implantation. Implants were well tolerated. CSF MHPG decreased about 75%, 5-HIAA 30%, and HVA from 30-50% with a tendency to plateau by the second week. Plasma MHPG decreased to undetectable levels. The findings demonstrate that long-term inhibition of MAO-A can be produced in unrestrained monkeys by minipump administered clorgyline. There is an apparently greater effect on the norepinephrine system relative to the serotonin and dopamine systems. C1 UNIV COLORADO,HLTH SCI CTR,DEPT PSYCHIAT,DENVER,CO 80262. NIMH,BETHESDA,MD 20892. FU NIMH NIH HHS [MH46335, MH19514] NR 9 TC 1 Z9 1 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD MAR PY 1991 VL 38 IS 3 BP 677 EP 679 DI 10.1016/0091-3057(91)90033-X PG 3 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA FD888 UT WOS:A1991FD88800033 PM 1712497 ER PT J AU PERRY, RR SMITH, PD EVANS, S PASS, HI AF PERRY, RR SMITH, PD EVANS, S PASS, HI TI INTRAVENOUS VS INTRAPERITONEAL SENSITIZER - IMPLICATIONS FOR INTRAPERITONEAL PHOTODYNAMIC THERAPY SO PHOTOCHEMISTRY AND PHOTOBIOLOGY LA English DT Article ID HEMATOPORPHYRIN DERIVATIVE DISTRIBUTION; TISSUE; TUMORS; CARCINOMA; CANCER; RADIOTHERAPY; TOXICITY; SARCOMAS; MOUSE AB Photodynamic therapy (PDT) is a potential treatment for peritoneal carcinomatosis. However, little data is available regarding the relative distribution of sensitizer to tumor and intra-abdominal organs, optimal route of sensitizer administration, and maximal tolerated light dose. Tumor and normal tissue sensitizer levels were measured by tissue extraction 3, 24, 48 and 72 h after 10 mg/kg of Photofrin II was given intraperitoneally (IP) or intravenously (IV) in a mouse peritoneal tumor model, and the maximal tolerated PDT light dose determined. Equivalent tumor sensitizer levels were obtained regardless of the route of sensitizer administration. Route of administration, however, did affect the kinetics of tumor sensitizer elimination, with the half-time for elimination (T1/2) 113.6 h for IP drug and 60.6 h for IV drug. Route of administration also affected sensitizer levels in several intra-abdominal organs, resulting in somewhat higher tumor to liver and kidney levels at 24 and 72 h after IP sensitizer administration. Despite these tissue distribution differences, route of sensitizer administration did not significantly affect PDT toxicity or mortality when mice were treated with 630 nm light. The maximum tolerated light dose was 1.04 J/cm2. These parameters will prove helpful in designing large scale animal trials assessing the efficacy and safety of intra-abdominal PDT. C1 NCI,SURG BRANCH,THORAC ONCOL SECT,BETHESDA,MD 20892. NIH,DIV RES SERV,BIOMED ENGN & INSTRUMENTAT BRANCH,BETHESDA,MD 20892. NR 28 TC 13 Z9 13 U1 0 U2 1 PU AMER SOC PHOTOBIOLOGY PI AUGUSTA PA BIOTECH PARK, 1021 15TH ST, SUITE 9, AUGUSTA, GA 30901-3158 SN 0031-8655 J9 PHOTOCHEM PHOTOBIOL JI Photochem. Photobiol. PD MAR PY 1991 VL 53 IS 3 BP 335 EP 340 DI 10.1111/j.1751-1097.1991.tb03637.x PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EZ997 UT WOS:A1991EZ99700007 PM 1829531 ER PT J AU CUTLER, JA GRANDITS, GA GRIMM, RH THOMAS, HE BILLINGS, JH WRIGHT, NH AF CUTLER, JA GRANDITS, GA GRIMM, RH THOMAS, HE BILLINGS, JH WRIGHT, NH TI RISK FACTOR CHANGES AFTER CESSATION OF INTERVENTION IN THE MULTIPLE RISK FACTOR INTERVENTION TRIAL SO PREVENTIVE MEDICINE LA English DT Article ID RANDOMIZED CONTROLLED TRIAL; DISEASE PREVENTION PROJECT; HEART-DISEASE; MEN C1 UNIV MINNESOTA,DEPT BIOSTAT,MINNEAPOLIS,MN 55455. UNIV MINNESOTA,DEPT EPIDEMIOL,MINNEAPOLIS,MN 55455. BOSTON UNIV,MED CTR,DEPT PREVENT MED & EPIDEMIOL,BOSTON,MA 02215. PACIFIC PRESBYTERIAN MED CTR,MED RES INST,SAN FRANCISCO,CA. UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT ENVIRONM & COMMUNITY MED,NEWARK,NJ 07103. RP CUTLER, JA (reprint author), NHLBI,DIV EPIDEMIOL & CLIN APPLICAT,ROOM 604 FED BLDG,BETHESDA,MD 20892, USA. NR 16 TC 28 Z9 28 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0091-7435 J9 PREV MED JI Prev. Med. PD MAR PY 1991 VL 20 IS 2 BP 183 EP 196 DI 10.1016/0091-7435(91)90019-Z PG 14 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA FD075 UT WOS:A1991FD07500001 PM 2057467 ER PT J AU GLYNN, TJ ANDERSON, DM SCHWARZ, L AF GLYNN, TJ ANDERSON, DM SCHWARZ, L TI TOBACCO-USE REDUCTION AMONG HIGH-RISK YOUTH - RECOMMENDATIONS OF A NATIONAL CANCER INSTITUTE EXPERT ADVISORY PANEL SO PREVENTIVE MEDICINE LA English DT Article ID 6-YEAR FOLLOW-UP; DRUG-USE; SMOKING; ADOLESCENTS; DROPOUTS C1 STANFORD UNIV,PALO ALTO,CA 94305. RP GLYNN, TJ (reprint author), NCI,EXECUT PLAZA N,ROOM 330,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 28 TC 70 Z9 70 U1 2 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0091-7435 J9 PREV MED JI Prev. Med. PD MAR PY 1991 VL 20 IS 2 BP 279 EP 291 DI 10.1016/0091-7435(91)90027-2 PG 13 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA FD075 UT WOS:A1991FD07500009 PM 2057474 ER PT J AU HAXBY, JV GRADY, CL HORWITZ, B UNGERLEIDER, LG MISHKIN, M CARSON, RE HERSCOVITCH, P SCHAPIRO, MB RAPOPORT, SI AF HAXBY, JV GRADY, CL HORWITZ, B UNGERLEIDER, LG MISHKIN, M CARSON, RE HERSCOVITCH, P SCHAPIRO, MB RAPOPORT, SI TI DISSOCIATION OF OBJECT AND SPATIAL VISUAL PROCESSING PATHWAYS IN HUMAN EXTRASTRIATE CORTEX SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE REGIONAL CEREBRAL BLOOD FLOW; POSITRON EMISSION TOMOGRAPHY ID POSITRON-EMISSION TOMOGRAPHY; POSTERIOR CEREBRAL-LESIONS; PET IMAGES; LOCALIZATION; ORGANIZATION AB The existence and neuroanatomical locations of separate extrastriate visual pathways for object recognition and spatial localization were investigated in healthy young men. Regional cerebral blood flow was measured by positron emission tomography and bolus injections of (H2O)-O-15, while subjects performed face matching, dot-location matching, or sensorimotor control tasks. Both visual matching tasks activated lateral occipital cortex. Face discrimination alone activated a region of occipitotemporal cortex that was anterior and inferior to the occipital area activated by both tasks. The spatial location task alone activated a region of lateral superior parietal cortex. Perisylvian and anterior temporal cortices were not activated by either task. These results demonstrate the existence of three functionally dissociable regions of human visual extrastriate cortex. The ventral and dorsal locations of the regions specialized for object recognition and spatial localization, respectively, suggest some homology between human and nonhuman primate extrastriate cortex, with displacement in human brain, possibly related to the evolution of phylogenetically newer cortical areas. C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. NIH,DEPT NUCL MED,POSITRON EMISS TOMOG SECT,BETHESDA,MD 20892. RP HAXBY, JV (reprint author), NIA,NEUROSCI LAB,BETHESDA,MD 20892, USA. RI Carson, Richard/H-3250-2011 OI Carson, Richard/0000-0002-9338-7966 NR 32 TC 692 Z9 697 U1 2 U2 16 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 5 BP 1621 EP 1625 DI 10.1073/pnas.88.5.1621 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA140 UT WOS:A1991FA14000007 PM 2000370 ER PT J AU GINSBERG, HS MOLDAWER, LL SEHGAL, PB REDINGTON, M KILIAN, PL CHANOCK, RM PRINCE, GA AF GINSBERG, HS MOLDAWER, LL SEHGAL, PB REDINGTON, M KILIAN, PL CHANOCK, RM PRINCE, GA TI A MOUSE MODEL FOR INVESTIGATING THE MOLECULAR PATHOGENESIS OF ADENOVIRUS PNEUMONIA SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE ADENOVIRUS EARLY GENES; CYTOKINES; CYTOTOXIC T-CELLS; NUDE MICE ID TUMOR NECROSIS FACTOR; CELL-SURFACE EXPRESSION; VIRUS; BETA-2-INTERFERON; ANTIGENS; DISEASE; INTERLEUKIN-1; INDUCTION; CYTOKINES; BIOLOGY AB Intranasal inoculation of type 5 adenovirus (Ad5) produced pneumonia in mice even though the virus did not replicate. To induce the pneumonia, however, a large viral infectious dose was required - i.e., 10(10) plaque-forming units. Four strains of inbred mouse were studied (C57BL/6N, C57BL/10ScN, CBA/N, and C3H/N): all showed similar inflammatory responses, although the greatest infiltration occurred in the C57BL/6N mice. The pathological response to Ad5 infection resembled that previously described in cotton rats: it consisted of overlapping early and late phases, and the infiltration contained primarily lymphocytes and monocytes/macrophages with a scattering of polymorphonuclear leukocytes. The prominent early phase and the presence of polymorphonuclear leukocytes suggested that induction of cytokines may play an important role in the pathogenesis of this pneumonia. Assays showed the appearance of tumor necrosis factor-alpha (TNF-alpha), interleukin 1(IL-1), and IL-6 in the infected mouse lungs concomitant with the developing early-phase infiltration. Only IL-6 was found in the peripheral blood. IL-6 reached maximum titers 6-24 hr after infection, whereas maximum levels of TNF-alpha and IL-1 were attained 2-3 days after infection. Specific RNAs for each of these cytokines were demonstrated in the infected lungs. To test the hypothesis that a cytotoxic T-cell response was responsible for the second phase, which primarily consisted of a perivascular and peribronchial infiltration of lymphocytes, Ad5 was used to infect C57BL/10ScN Nu/Nu and parent mice. The nude mice showed a normal early-phase response, but essentially no peribronchial and only minimal perivascular infiltrations occurred. C1 COLUMBIA UNIV COLL PHYS & SURG,DEPT MICROBIOL,NEW YORK,NY 10032. CORNELL UNIV,MED CTR,COLL MED,DEPT SURG,NEW YORK,NY 10021. ROCKEFELLER UNIV,NEW YORK,NY 10021. NIAID,ORGAN CHEM LAB,BETHESDA,MD 20892. HOFFMANN LA ROCHE INC,DEPT IMMUNOPHARMACOL,NUTLEY,NJ 07111. JOHNS HOPKINS UNIV,SCH HYG,DEPT INT HLTH,BALTIMORE,MD 21205. RP GINSBERG, HS (reprint author), COLUMBIA UNIV COLL PHYS & SURG,DEPT MED,NEW YORK,NY 10032, USA. FU NCI NIH HHS [CA-44365]; NIAID NIH HHS [AI-12052]; NIGMS NIH HHS [GM-40586] NR 26 TC 314 Z9 317 U1 1 U2 3 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 5 BP 1651 EP 1655 DI 10.1073/pnas.88.5.1651 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA140 UT WOS:A1991FA14000013 PM 1848005 ER PT J AU WALL, RJ PURSEL, VG SHAMAY, A MCKNIGHT, RA PITTIUS, CW HENNIGHAUSEN, L AF WALL, RJ PURSEL, VG SHAMAY, A MCKNIGHT, RA PITTIUS, CW HENNIGHAUSEN, L TI HIGH-LEVEL SYNTHESIS OF A HETEROLOGOUS MILK PROTEIN IN THE MAMMARY-GLANDS OF TRANSGENIC SWINE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID WHEY ACIDIC PROTEIN; TISSUE PLASMINOGEN-ACTIVATOR; GENE PROMOTER; HORMONAL-REGULATION; CASEIN GENE; EXPRESSION; MICE; MOUSE; RAT; LIVESTOCK AB The whey acidic protein (WAP) is a major milk protein in mice, rats, and rabbits but has not been found in milk of livestock including swine. To determine whether mammary gland regulatory elements from the WAP gene function across species boundaries and whether it is possible to qualitatively alter milk protein composition, we introduced the mouse WAP gene into the genome of swine. Three lines of transgenic swine were analyzed, and mouse WAP was detected in milk from all lactating females at concentrations of about 1 g/liter; these levels are similar to those found in mouse milk. Expression of the corresponding RNA was specific to the mammary gland. Our results suggest that the molecular basis of mammary-specific gene expression is conserved between swine and mouse. In addition the WAP gene must share, with other milk protein genes, elements that target gene expression to the mammary gland. Mouse WAP accounted for about 3% of the total milk proteins in transgenic pigs, thus demonstrating that it is possible to produce high levels of a foreign protein in milk of farm animals. C1 USDA ARS,BELTSVILLE AGR RES CTR,REPROD LAB,BELTSVILLE,MD 20705. NIDDKD,BIOCHEM & METAB LAB,BETHESDA,MD 20892. NR 36 TC 121 Z9 128 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 5 BP 1696 EP 1700 DI 10.1073/pnas.88.5.1696 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA140 UT WOS:A1991FA14000022 PM 1705703 ER PT J AU OSHEA, JJ ASHWELL, JD BAILEY, TL CROSS, SL SAMELSON, LE KLAUSNER, RD AF OSHEA, JJ ASHWELL, JD BAILEY, TL CROSS, SL SAMELSON, LE KLAUSNER, RD TI EXPRESSION OF V-SRC IN A MURINE T-CELL HYBRIDOMA RESULTS IN CONSTITUTIVE T-CELL RECEPTOR PHOSPHORYLATION AND INTERLEUKIN-2 PRODUCTION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE ONCOGENE; PROTEIN-TYROSINE KINASE; LYMPHOKINE; SIGNAL TRANSDUCTION; PROTEIN KINASE-C ID ANTIGEN RECEPTOR; PHOSPHOINOSITIDE HYDROLYSIS; MONOCLONAL-ANTIBODIES; ACTIVATION; COMPLEX; PROTEIN; KINASES; VECTOR; CHAIN AB Ligand binding to the T-cell antigen receptor results in phosphatidylinositol hydrolysis and the resultant activation of protein kinase C, as well as the activation of a receptor-coupled protein-tyrosine kinase. As a model for tyrosine kinase activation in T cells, we used retroviral gene transfer to express the v-src oncogene in an antigen-specific murine T-cell hybridoma. Clones that expressed v-src mRNA demonstrated constitutive tyrosine phosphorylation of several cellular substrates, including the zeta-chain of the T-cell receptor, and constitutive interleukin 2 production. Thus, expression of a constitutively active protein-tyrosine kinase such as pp60v-src appears to be sufficient to induce the expression of at least one gene critical to the process of T-cell activation. C1 NCI,FREDERICK CANC RES FACIL,DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892. RP OSHEA, JJ (reprint author), NCI,FREDERICK CANC RES FACIL,DYNCORP,PROGRAM RESOURCES INC,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701, USA. FU NCI NIH HHS [N01-CO-74102] NR 29 TC 36 Z9 36 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 5 BP 1741 EP 1745 DI 10.1073/pnas.88.5.1741 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA140 UT WOS:A1991FA14000031 PM 2000381 ER PT J AU CHRISTY, BA SANDERS, LK LAU, LF COPELAND, NG JENKINS, NA NATHANS, D AF CHRISTY, BA SANDERS, LK LAU, LF COPELAND, NG JENKINS, NA NATHANS, D TI AN ID-RELATED HELIX LOOP HELIX PROTEIN ENCODED BY A GROWTH FACTOR-INDUCIBLE GENE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE INHIBITOR OF DNA BINDING; TRANSCRIPTION FACTOR; CELL PROLIFERATION; MOUSE CHROMOSOME-4 ID C-FOS; CELLS; MYC; IDENTIFICATION; TRANSCRIPTION; LOCALIZATION; EXPRESSION; SEQUENCES; ONCOGENES; BINDING AB An mRNA encoding a helix-loop-helix protein that we have named HLH462 is induced in mouse 3T3 cells as part of the immediate early transcriptional response to growth factors and other signaling agents. The RNA is present in a number of mouse tissues and in the developing mouse fetus. The HLH462 gene has been mapped by interspecific backcross analysis to the distal region of mouse chromosome 4. In its helix-loop-helix region HLH462 is closely related to the Id protein and the Drosophila emc protein. Like Id, HLH462 lacks a basic region required for DNA binding, and it inhibits the DNA-binding activities of other helix-loop-helix proteins. On the basis of its structural and functional similarity to Id, we suggest that HLH462 may inhibit the activities of helix-loop-helix transcription factors during the cellular growth response and during development. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT MOLEC BIOL & GENET,BALTIMORE,MD 21205. NCI,FREDERICK CANC RES FACIL,MAMMALIAN GENET LAB,FREDERICK,MD 21701. RP CHRISTY, BA (reprint author), JOHNS HOPKINS UNIV,SCH MED,HOWARD HUGHES MED INST,BALTIMORE,MD 21205, USA. FU NCI NIH HHS [CA16519, N01-CO-74101] NR 29 TC 324 Z9 336 U1 0 U2 3 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 5 BP 1815 EP 1819 DI 10.1073/pnas.88.5.1815 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA140 UT WOS:A1991FA14000047 PM 2000388 ER PT J AU WEINER, DM LEVEY, AI SUNAHARA, RK NIZNIK, HB ODOWD, BF SEEMAN, P BRANN, MR AF WEINER, DM LEVEY, AI SUNAHARA, RK NIZNIK, HB ODOWD, BF SEEMAN, P BRANN, MR TI D1 AND D2 DOPAMINE RECEPTOR MESSENGER-RNA IN RAT-BRAIN SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE STRIATUM; SUBSTANTIA NIGRA; AMYGDALA; SEPTUM; INSITU HYBRIDIZATION ID CATECHOLAMINE INNERVATION; BASAL FOREBRAIN; AUTORADIOGRAPHIC LOCALIZATION; ADENYLATE-CYCLASE; CORTEX; ORGANIZATION; EXPRESSION; SYSTEM AB Physiological and pharmacological criteria have divided dopamine receptors into D1 and D2 subtypes, and genes encoding these subtypes have recently been cloned. Based on the sequences of the cloned receptors, we prepared oligodeoxynucleotide probes to map the cellular expression of the corresponding mRNAs in rat brain by in situ hybridization histochemistry. These mRNAs showed largely overlapping yet distinct patterns of expression. The highest levels of expression for both mRNAs were observed in the caudate-putamen, nucleus accumbens, and olfactory tubercle. Within the caudate-putamen, 47 +/- 6% and 46 +/- 5% of the medium-sized neurons (10-15-mu-m) expressed the D1 and D2 mRNAs, respectively, and only the D2 mRNA was observed in the larger neurons (> 20-mu-m). The D1 and D2 mRNAs were expressed in most cortical regions, with the highest levels in the prefrontal and entorhinal cortices. Within neocortex, D1 mRNA was observed primarily in layer 6 and D2 mRNA in layers 4-5. Within the amygdala, D1 mRNA was observed in the intercalated nuclei, and D2 mRNA in the central nucleus. Within the hypothalamus, D1 mRNA was observed in the suprachiasmatic nucleus and D2 mRNA in many of the dopaminergic cell groups. Within the septum, globus pallidus, superior and inferior colliculi, mammillary bodies, and substantia nigra only D2 mRNA was detected. These data provide insight into the neuroanatomical basis of the differential effects of drugs that act on D1 or D2 receptors. C1 UNIV TORONTO,ADDICT RES CTR,DEPT PHARMACOL,TORONTO M5S 2S1,ONTARIO,CANADA. UNIV TORONTO,ADDICT RES CTR,DEPT PSYCHIAT,TORONTO M5S 2S1,ONTARIO,CANADA. NINCDS,MOLEC BIOL LAB,BETHESDA,MD 20892. NIH,HOWARD HUGHES MED INST,RES SCHOLARS PROGRAM,BALTIMORE,MD 21205. FU NINDS NIH HHS [NS01387-01] NR 40 TC 377 Z9 382 U1 0 U2 3 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 5 BP 1859 EP 1863 DI 10.1073/pnas.88.5.1859 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA140 UT WOS:A1991FA14000056 PM 1825729 ER PT J AU MERCER, WE SHIELDS, MT LIN, D APPELLA, E ULLRICH, SJ AF MERCER, WE SHIELDS, MT LIN, D APPELLA, E ULLRICH, SJ TI GROWTH SUPPRESSION INDUCED BY WILD-TYPE P53-PROTEIN IS ACCOMPANIED BY SELECTIVE DOWN-REGULATION OF PROLIFERATING-CELL NUCLEAR ANTIGEN EXPRESSION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE GENE EXPRESSION; CELL CYCLE REGULATION; TUMOR SUPPRESSOR ID DNA POLYMERASE-DELTA; MONOCLONAL-ANTIBODIES; P53 GENE; REPLICATION INVITRO; AUXILIARY PROTEIN; TUMOR-ANTIGEN; MESSENGER-RNA; T-ANTIGEN; CYCLIN; PCNA AB The p53 gene is a frequent target of mutation in a wide variety of human cancers. Previously, it was reported that conditional expression of wild-type p53 protein in a cell line (GM47.23) derived from a human glioblastoma multiform tumor had a negative effect on cell proliferation. We have now investigated the effect that induction of wild-type p53 protein in this cell line has on the expression of the proliferating-cell nuclear antigen gene. The proliferating-cell nuclear antigen gene encodes a nuclear protein that is an auxiliary factor of DNA polymerase-delta and part of the DNA replication machinery of the cell. We show that inhibition of cell cycle progression into S-phase after induction of wild-type p53 protein is accompanied by selective down-regulation of proliferating-cell nuclear antigen mRNA and protein expression. C1 TEMPLE UNIV,HLTH SCI CTR,SCH MED,DEPT PATHOL,PHILADELPHIA,PA 19140. TEMPLE UNIV,HLTH SCI CTR,SCH MED,FELS RES INST,PHILADELPHIA,PA 19140. NCI,CELL BIOL LAB,BETHESDA,MD 20892. FU NCI NIH HHS [CA42866, CA 09644] NR 48 TC 273 Z9 278 U1 1 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 5 BP 1958 EP 1962 DI 10.1073/pnas.88.5.1958 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA140 UT WOS:A1991FA14000076 PM 1705714 ER PT J AU HORAK, ID GRESS, RE LUCAS, PJ HORAK, EM WALDMANN, TA BOLEN, JB AF HORAK, ID GRESS, RE LUCAS, PJ HORAK, EM WALDMANN, TA BOLEN, JB TI LYMPHOCYTE-T INTERLEUKIN-2-DEPENDENT TYROSINE PROTEIN-KINASE SIGNAL TRANSDUCTION INVOLVES THE ACTIVATION OF P56LCK SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE INTERLEUKIN-2 RECEPTOR; TYROSINE PHOSPHORYLATION ID BETA-CHAIN; IL-2 RECEPTOR; CDNA; PHOSPHORYLATION; PROLIFERATION; CELLS; GENE; P70 AB Addition of interleukin 2 (IL-2) to IL-2-dependent T cells results in tyrosine protein kinase signal transduction events even though the IL-2 receptor-alpha and beta chains lack intrinsic enzymatic activity. Here we report that addition of IL-2 to IL-2-dependent human T cells transiently stimulates the specific activity of p56lck, a member of the src family of nonreceptor tyrosine protein kinases expressed at high levels in T lymphocytes. The ability of IL-2 to induce p56lck activation was found to be independent of the capacity of p56lck to associate with either CD4 or CD8. Following IL-2 treatment, p56lck was found to undergo serine/threonine phosphorylation modifications that resulted in altered mobility of the lck gene product on polyacrylamide gels. These observations raise the possibility that p56lck participates in IL-2-mediated signal transduction events in T cells. C1 NCI,TUMOR VIRUS BIOL LAB,BLDG 41,ROOM D-824,BETHESDA,MD 20892. NCI,PHARMACOL BRANCH,BETHESDA,MD 20892. NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. NCI,METAB BRANCH,BETHESDA,MD 20892. NR 33 TC 256 Z9 257 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 5 BP 1996 EP 2000 DI 10.1073/pnas.88.5.1996 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA140 UT WOS:A1991FA14000084 PM 2000405 ER PT J AU TORTORA, G YOKOZAKI, H PEPE, S CLAIR, T CHOCHUNG, YS AF TORTORA, G YOKOZAKI, H PEPE, S CLAIR, T CHOCHUNG, YS TI DIFFERENTIATION OF HL-60 LEUKEMIA BY TYPE-I REGULATORY SUBUNIT ANTISENSE OLIGODEOXYNUCLEOTIDE OF CAMP-DEPENDENT PROTEIN-KINASE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE CAMP KINASE ID AMINO-ACID SEQUENCE; MOLECULAR-CLONING; CATALYTIC SUBUNIT; GENE-EXPRESSION; HUMAN TESTIS; MONOCLONAL-ANTIBODIES; ANTIGENIC EXPRESSION; HUMAN-MONOCYTES; CDNA STRUCTURE; CELLS AB A marked decrease in the type I cAMP-dependent protein kinase regulatory subunit (RI-alpha) and an increase in the type II protein kinase regulatory subunit (RII-beta) correlate with growth inhibition and differentiation induced in a variety of types of human cancer cells, in vitro and in vivo, by site-selective cAMP analogs. To directly determine whether RI-alpha is a growth-inducing protein essential for neoplastic cell growth, human HL-60 promyelocytic leukemia cells were exposed to 21-mer RI-alpha antisense oligodeoxynucleotide, and the effects on cell replication and differentiation were examined. The RI-alpha antisense oligomer brought about growth inhibition and monocytic differentiation, bypassing the effects of an exogenous cAMP analog. These effects of RI-alpha antisense oligodeoxynucleotide correlated with a decrease in RI-alpha receptor and an increase in RII-beta receptor level. The growth inhibition and differentiation were abolished, however, when these cells were exposed simultaneously to both RI-alpha and RII-beta antisense oligodeoxynucleotides. The RII-beta antisense oligodeoxynucleotide alone has been previously shown to specifically block the differentiation inducible by cAMP analogs. These results provide direct evidence that RI-alpha cAMP receptor plays a critical role in neoplastic cell growth and that cAMP receptor isoforms display specific roles in cAMP regulation of cell growth and differentiation. C1 NCI,TUMOR IMMUNOL & BIOL LAB,CELLULAR BIOCHEM SECT,BLDG 10,ROOM 5B38,BETHESDA,MD 20892. OI Yokozaki, Hiroshi/0000-0001-5276-3331 NR 39 TC 113 Z9 114 U1 1 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 5 BP 2011 EP 2015 DI 10.1073/pnas.88.5.2011 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FA140 UT WOS:A1991FA14000087 PM 2000408 ER PT J AU TAKETO, M SCHROEDER, AC MOBRAATEN, LE GUNNING, KB HANTEN, G FOX, RR RODERICK, TH STEWART, CL LILLY, F HANSEN, CT OVERBEEK, PA AF TAKETO, M SCHROEDER, AC MOBRAATEN, LE GUNNING, KB HANTEN, G FOX, RR RODERICK, TH STEWART, CL LILLY, F HANSEN, CT OVERBEEK, PA TI FVB/N - AN INBRED MOUSE STRAIN PREFERABLE FOR TRANSGENIC ANALYSES SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE ES CELLS; BLASTOCYST CHIMERA; GERM-LINE TRANSMISSION ID MICE; DNA; GENE; EXPRESSION; CELLS; LINE; LENS AB FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J X SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice. C1 BAYLOR UNIV,DEPT CELL BIOL,HOWARD HUGHES MED INST,HOUSTON,TX 77030. JACKSON LAB,BAR HARBOR,ME 04609. ROCHE INST MOLEC BIOL,DEPT CELL & DEV BIOL,NUTLEY,NJ 07110. YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MOLEC GENET,BRONX,NY 10461. NCI,BETHESDA,MD 20892. FU NCI NIH HHS [CA 02662, CA 39652]; NICHD NIH HHS [HD 21970] NR 26 TC 443 Z9 447 U1 1 U2 11 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 6 BP 2065 EP 2069 DI 10.1073/pnas.88.6.2065 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FC216 UT WOS:A1991FC21600007 PM 1848692 ER PT J AU WAHLESTEDT, C KAROUM, F JASKIW, G WYATT, RJ LARHAMMAR, D EKMAN, R REIS, DJ AF WAHLESTEDT, C KAROUM, F JASKIW, G WYATT, RJ LARHAMMAR, D EKMAN, R REIS, DJ TI COCAINE-INDUCED REDUCTION OF BRAIN NEUROPEPTIDE-Y SYNTHESIS DEPENDENT ON MEDIAL PREFRONTAL CORTEX SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID CENTRAL NERVOUS-SYSTEM; DOPAMINE; NUCLEUS; REINFORCEMENT; AMPHETAMINE; NEURONS; FLUID AB Repeated administration of cocaine elicits substantial, long-lasting, but reversible reductions in neuropeptide Y (NPY) and NPY mRNA in the rat cerebral cortex and nucleus accumbens. The NPY reduction appears to be mediated through a decrease in NPY biosynthesis, occurring transneuronally, perhaps in response to changes in synaptic dopamine associated with mesolimbic and mesocortical dopamine neurons. The medial prefrontal cortex appears necessary for maintenance of cocaine's action on this neuronal network since excitotoxic lesions of this area prevented (lesion before cocaine) and reversed (lesion after cocaine) the reductions in NPY elicited by the cocaine. NPY may be a sensitive marker for chronic cocaine use. Its decrease may relate to the anxiety and depression associated with cocaine withdrawal in humans. C1 ST ELIZABETH HOSP,NIMH,INTRAMURAL RES PROGRAM,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. ST ELIZABETH HOSP,NIMH,INTRAMURAL RES PROGRAM,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. UNIV LUND,DEPT PSYCHIAT & NEUROCHEM,S-22101 LUND,SWEDEN. UNIV UPPSALA,DEPT MOLEC GENET,S-75105 UPPSALA,SWEDEN. RP WAHLESTEDT, C (reprint author), CORNELL UNIV,MED CTR,COLL MED,DEPT NEUROL & NEUROSCI,DIV NEUROBIOL,411 E 69TH ST,NEW YORK,NY 10021, USA. RI Wahlestedt, Claes/A-7039-2009 NR 38 TC 74 Z9 75 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 6 BP 2078 EP 2082 DI 10.1073/pnas.88.6.2078 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FC216 UT WOS:A1991FC21600010 PM 2006146 ER PT J AU BRIGHTMAN, BK REIN, A TREPP, DJ FAN, H AF BRIGHTMAN, BK REIN, A TREPP, DJ FAN, H TI AN ENHANCER VARIANT OF MOLONEY MURINE LEUKEMIA-VIRUS DEFECTIVE IN LEUKEMOGENESIS DOES NOT GENERATE DETECTABLE MINK CELL FOCUS-INDUCING VIRUS INVIVO SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE PRELEUKEMIA ID LONG TERMINAL REPEAT; M-MULV; C-MYC; SEQUENCES; INTEGRATION; PROVIRUSES; FREQUENCY; FRIEND; MICE AB Moloney murine leukemia virus (Mo-MuLV) induces T-cell lymphoma when inoculated into neonatal mice. This is a multistep process. Early events observed in infected mice include generalized hematopoietic hyperplasia in the spleen and appearance of mink cell focus-inducing (MCF) recombinants; end-stage tumors are characterized by insertional proviral activation of protooncogenes. We previously showed that an Mo-MuLV enhancer variant, Mo+PyF101 Mo-MuLV, has greatly reduced leukemogenicity and is deficient in induction of preleukemic hyperplasia. In this report, we have examined Mo+PyF101 Mo-MuLV-inoculated mice for the presence of MCF recombinants. In contrast to wild-type Mo-MuLV-inoculated mice, Mo+PyF101 Mo-MuLV-inoculated mice did not generate detectable MCF recombinants. This failure was at least partly due to an inability of the MCF virus to propagate in vivo, since a molecularly cloned infectious Mo+PyF101 MCF virus did not replicate, even when inoculated as a Mo+PyF101 Mo-MuLV pseudotype. These results show that the leukemogenic defect of Mo+PyF101 Mo-MuLV is associated with its inability to generate MCF recombinants capable of replication in vivo. This, in turn, is consistent with the view that MCF recombinants play a significant role in Mo-MuLV-induced disease and, in particular, may play a role early in the disease process. C1 UNIV CALIF IRVINE,CANC RES INST,IRVINE,CA 92717. NCI,FREDERICK CANC RES & DEV CTR,BASIC RES PROGRAM,ADV BIOSCI LABS INC,FREDERICK,MD 21701. RP BRIGHTMAN, BK (reprint author), UNIV CALIF IRVINE,DEPT MOLEC BIOL & BIOCHEM,IRVINE,CA 92717, USA. FU NCI NIH HHS [R0I CA32455, N01-CO-74101]; NIAID NIH HHS [T32 AI07319] NR 21 TC 40 Z9 40 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR PY 1991 VL 88 IS 6 BP 2264 EP 2268 DI 10.1073/pnas.88.6.2264 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FC216 UT WOS:A1991FC21600049 PM 2006167 ER PT J AU KASPER, S ROSENTHAL, NE BARBERI, S WILLIAMS, A TAMARKIN, L ROGERS, SLB PILLEMER, SR AF KASPER, S ROSENTHAL, NE BARBERI, S WILLIAMS, A TAMARKIN, L ROGERS, SLB PILLEMER, SR TI IMMUNOLOGICAL CORRELATES OF SEASONAL FLUCTUATIONS IN MOOD AND BEHAVIOR AND THEIR RELATIONSHIP TO PHOTOTHERAPY SO PSYCHIATRY RESEARCH LA English DT Article DE IMMUNE FUNCTION; LYMPHOCYTE BLASTOGENESIS; LYMPHOCYTE SUBTYPES; DEPRESSION; SEASONAL AFFECTIVE DISORDERS; PHOTOTHERAPY ID AFFECTIVE-DISORDER; IMMUNE FUNCTION; LIGHT THERAPY; DEPRESSION; CELLS AB Immunological parameters were studied before and after phototherapy, with bright and dim light, in 38 individuals with a range of retrospectively reported seasonal changes in mood and behavior. There was a significant negative correlation between the degree of mood and behavioral difficulties in fall and winter (seasonality) and the total number of circulating natural killer cells. Changes in the numbers of circulating helper T cells correlated significantly with changes in mood following phototherapy. Moreover, mitogen-induced lymphocyte blastogenesis increased significantly after phototherapy, but there was no significant difference between the bright and dim light treatments. The results suggest that cellular immune function is associated with both seasonality and response to phototherapy. C1 NIAMSD,OFF PREVENT EPIDEMIOL & CLIN APPLICAT,BETHESDA,MD. NIMH,OUTPATIENT STUDIES UNIT,BETHESDA,MD 20892. NIMH,CLIN PSYCHOBIOL BRANCH,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. NR 33 TC 9 Z9 10 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD MAR PY 1991 VL 36 IS 3 BP 253 EP 264 DI 10.1016/0165-1781(91)90024-J PG 12 WC Psychiatry SC Psychiatry GA FF525 UT WOS:A1991FF52500002 PM 2062967 ER PT J AU BARONE, S NANRY, KP MUNDY, WR MCGINTY, JF TILSON, HA AF BARONE, S NANRY, KP MUNDY, WR MCGINTY, JF TILSON, HA TI SPATIAL-LEARNING DEFICITS ARE NOT SOLELY DUE TO CHOLINERGIC DEFICITS FOLLOWING MEDIAL SEPTAL-LESIONS WITH COLCHICINE SO PSYCHOBIOLOGY LA English DT Article ID NUCLEUS BASALIS-MAGNOCELLULARIS; RAT-BRAIN; NEURONS; FOREBRAIN; MEMORY; PROJECTIONS; ACETYLTRANSFERASE; PERFORMANCE; HIPPOCAMPUS; CONNECTIONS AB Colchicine was infused bilaterally into the cerebrolateral ventricles (3.75-mu-g/side) or directly into the medial septum (5-mu-g) of adult, male Fischer-344 rats (n = 48), and effects on behavior and cholinergic markers were determined. Rats receiving intracerebroventricular (ICV) administration of colchicine were hyperaggressive during the first week after administration and were hyperactive when tested during 60-min sessions at weekly intervals during the first 3 weeks after colchicine treatment. ICV colchicine also interfered with the acquisition of a spatial task in the water maze. Rats receiving colchicine directly into the medial septum were also aggressive and hyperactive, but were not impaired in the acquisition of the water-maze task. It was subsequently found that direct administration and ICV administration of colchicine both decreased the number of choline acetyltransferase (ChAT) immunoreactive cells in the medial septum by at least 50% of vehicle-treated rats and decreased ChAT enzyme activity in both the right and the left hippocampus to about 50% of control levels. However, the acetylcholinesterase (AChE) staining was more clearly decreased bilaterally in the hippocampus after ICV infusion than after direct infusion of colchicine. ICV infusion resulted in bilateral shrinkage of the lateral septum, whereas direct septal administration resulted in only unilateral damage to the lateral septum. The results of these experiments do not support the generally accepted hypothesis that spatial learning deficits seen in animals with medial septum lesions are solely due to a lesion-associated cholinergic deficit in the hippocampus. C1 NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,RES TRIANGLE PK,NC 27709. E CAROLINA UNIV,SCH MED,GREENVILLE,NC 27834. NR 34 TC 18 Z9 18 U1 0 U2 0 PU PSYCHONOMIC SOC INC PI AUSTIN PA 1710 FORTVIEW RD, AUSTIN, TX 78704 SN 0889-6313 J9 PSYCHOBIOLOGY JI Psychobiology PD MAR PY 1991 VL 19 IS 1 BP 41 EP 50 PG 10 WC Psychology; Psychology, Multidisciplinary SC Psychology GA FD256 UT WOS:A1991FD25600004 ER PT J AU LIPMAN, PD AF LIPMAN, PD TI AGE AND EXPOSURE DIFFERENCES IN ACQUISITION OF ROUTE INFORMATION SO PSYCHOLOGY AND AGING LA English DT Article ID LANDMARKS RP LIPMAN, PD (reprint author), NIMH,FED BLDG,ROOM B1A-14,7550 WISCONSIN AVE,BETHESDA,MD 20892, USA. NR 13 TC 36 Z9 36 U1 2 U2 3 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0882-7974 J9 PSYCHOL AGING JI Psychol. Aging PD MAR PY 1991 VL 6 IS 1 BP 128 EP 133 DI 10.1037//0882-7974.6.1.128 PG 6 WC Gerontology; Psychology, Developmental SC Geriatrics & Gerontology; Psychology GA FA553 UT WOS:A1991FA55300016 PM 2029361 ER PT J AU ENGEL, BT TALAN, MI AF ENGEL, BT TALAN, MI TI HEMODYNAMIC AND RESPIRATORY CONCOMITANTS OF LEARNED HEART-RATE CONTROL DURING EXERCISE SO PSYCHOPHYSIOLOGY LA English DT Article DE OPERANT CONDITIONING; HEART RATE; EXERCISE; HEMODYNAMICS; RESPIRATION ID NONHUMAN-PRIMATES; RESPONSES AB Each of three monkeys was trained to slow its heart, to exercise (lift weights), and to attenuate the tachycardia of exercise by combining these two skills. During all experiments, heart rate, stroke volume, intra-arterial blood pressure, O2 consumption, and CO2 production were recorded on a beat-to-beat basis. All animals reliably attenuated the tachycardia of exercise, indicating that this expression of central command is, at least in part, a learned motor skill. Double-product (heart rate x systolic pressure) was attenuated during combined sessions relative to exercise only sessions, and heart rate was always lower at similar levels of cardiac output, indicating that under the combined conditions, animals were performing with better cardiac efficiency at comparable levels of mechanical effort. Analyses of O2 consumption, CO2 production, and respiratory quotient (the ratio of CO2 production to O2 consumption) suggested that the animals also might have been delivering more O2 to their working muscles during combined sessions. RP ENGEL, BT (reprint author), NIA,GERONTOL RES CTR,BEHAV SCI LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 13 TC 6 Z9 6 U1 0 U2 0 PU SOC PSYCHOPHYSIOL RES PI WASHINGTON PA 1010 VERMONT AVE NW SUITE 1100, WASHINGTON, DC 20005 SN 0048-5772 J9 PSYCHOPHYSIOLOGY JI Psychophysiology PD MAR PY 1991 VL 28 IS 2 BP 225 EP 230 DI 10.1111/j.1469-8986.1991.tb00414.x PG 6 WC Psychology, Biological; Neurosciences; Physiology; Psychology; Psychology, Experimental SC Psychology; Neurosciences & Neurology; Physiology GA GD533 UT WOS:A1991GD53300011 PM 1946888 ER PT J AU ALMADA, SJ ZONDERMAN, AB SHEKELLE, RB DYER, AR DAVIGLUS, ML COSTA, PT STAMLER, J AF ALMADA, SJ ZONDERMAN, AB SHEKELLE, RB DYER, AR DAVIGLUS, ML COSTA, PT STAMLER, J TI NEUROTICISM AND CYNICISM AND RISK OF DEATH IN MIDDLE-AGED MEN - THE WESTERN ELECTRIC STUDY SO PSYCHOSOMATIC MEDICINE LA English DT Article ID CORONARY-DISEASE; PERSONALITY; HOSTILITY; MORTALITY AB MMPI measures of neuroticism (N) and of cynicism (C) were obtained at the initial examination of 1871 employed, middle-aged men in Chicago. Neither N nor C was significantly associated cross-sectionally with systolic blood pressure or serum cholesterol, but both were positively associated with cigarette smoking and alcohol consumption. N was not significantly associated with risk of death from coronary disease, other cardiovascular diseases, cancer, all other causes combined, or all combined after adjustment for C, age, cigarette smoking, alcohol consumption, systolic blood pressure, and serum cholesterol. C was significantly associated with coronary death and total mortality after adjustment for N and the other variables listed above, and relative risks of 1.4 to 1.6 were observed for all endpoints. These results support the idea that neuroticism is not associated with major causes of mortality, but that cynicism is associated with several causes. The associations between cynicism and mortality may be mediated by cigarette smoking and excessive alcohol consumption since adjustment for these factors may have been incomplete due to correlation between cynicism and errors in self-reported data. C1 NORTHWESTERN UNIV,SCH MED,DEPT COMMUNITY HLTH & PREVENT MED,680 N LAKE SHORE DR,CHICAGO,IL 60611. UNIV TEXAS,HLTH SCI CTR,SCH PUBL HLTH,HOUSTON,TX 77225. NIA,FRANCIS SCOTT KEY MED CTR,GERONTOL RES CTR,BALTIMORE,MD 21224. OI Costa, Paul/0000-0003-4375-1712; Zonderman, Alan B/0000-0002-6523-4778 FU NCI NIH HHS [CA 38326]; NHLBI NIH HHS [HL 07113, HL 21010] NR 21 TC 104 Z9 104 U1 1 U2 7 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD MAR-APR PY 1991 VL 53 IS 2 BP 165 EP 175 PG 11 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA FB284 UT WOS:A1991FB28400006 PM 2031070 ER PT J AU HAYTHORNTHWAITE, JA ANDERSON, DE PRATLEY, RE AF HAYTHORNTHWAITE, JA ANDERSON, DE PRATLEY, RE TI THE SEPARATE AND COMBINED EFFECTS OF STRESS AND HIGH SODIUM-INTAKE ON RESTING BLOOD-PRESSURE SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD MAR-APR PY 1991 VL 53 IS 2 BP 232 EP 232 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA FB284 UT WOS:A1991FB28400070 ER PT J AU REITE, M LAUDENSLAGER, M GARRICK, N BOCCIA, M COX, M AF REITE, M LAUDENSLAGER, M GARRICK, N BOCCIA, M COX, M TI CLORGYLINE EFFECTS ON MATERNAL SEPARATION IN PIGTAIL (MACACA-NEMESTRINA) MONKEY INFANTS SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract C1 UNIV COLORADO,SCH MED,DENVER,CO 80262. NIMH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD MAR-APR PY 1991 VL 53 IS 2 BP 233 EP 234 PG 2 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA FB284 UT WOS:A1991FB28400074 ER PT J AU STOTTS, RC AF STOTTS, RC TI APPLICATION OF THE SALMON MODEL - A TALE OF 2 CITIES SO PUBLIC HEALTH NURSING LA English DT Article C1 UNIV TEXAS,MED BRANCH,GALVESTON,TX 77550. RP STOTTS, RC (reprint author), NCI,DIV CANC PREVENT & CONTROL,EXECUT PLAZA S,ROOM T-41,BETHESDA,MD 20892, USA. NR 8 TC 2 Z9 2 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0737-1209 J9 PUBLIC HEALTH NURS JI Public Health Nurs. PD MAR PY 1991 VL 8 IS 1 BP 10 EP 14 PG 5 WC Public, Environmental & Occupational Health; Nursing SC Public, Environmental & Occupational Health; Nursing GA EY905 UT WOS:A1991EY90500003 PM 2023850 ER PT J AU RON, E MODAN, B PRESTON, D ALFANDARY, E STOVALL, M BOICE, JD AF RON, E MODAN, B PRESTON, D ALFANDARY, E STOVALL, M BOICE, JD TI RADIATION-INDUCED SKIN CARCINOMAS OF THE HEAD AND NECK SO RADIATION RESEARCH LA English DT Article ID CANCER; IRRADIATION; MORTALITY; TUMORS; SCALP; RISK C1 CHAIM SHEBA MED CTR,DEPT CLIN EPIDEMIOL,IL-52621 TEL HASHOMER,ISRAEL. RADIAT EFFECTS RES FDN,DEPT STAT,HIROSHIMA 732,JAPAN. UNIV TEXAS,MD ANDERSON CANCER CTR,HOUSTON,TX 77030. RP RON, E (reprint author), NCI,RADIAT EPIDEMIOL BRANCH,DIV CANC ETIOL,EXECUT PLAZA N,SUITE 408,BETHESDA,MD 20892, USA. NR 31 TC 106 Z9 108 U1 1 U2 3 PU RADIATION RESEARCH SOC PI OAK BROOK PA 2021 SPRING RD, STE 600, OAK BROOK, IL 60521 SN 0033-7587 J9 RADIAT RES JI Radiat. Res. PD MAR PY 1991 VL 125 IS 3 BP 318 EP 325 DI 10.2307/3578117 PG 8 WC Biology; Biophysics; Radiology, Nuclear Medicine & Medical Imaging SC Life Sciences & Biomedicine - Other Topics; Biophysics; Radiology, Nuclear Medicine & Medical Imaging GA FB507 UT WOS:A1991FB50700014 PM 2000456 ER PT J AU LONDON, JF SHAWKER, TH DOPPMAN, JL FRUCHT, HH VINAYEK, R STARK, HA MILLER, LS MILLER, DL NORTON, JA JENSEN, RT GARDNER, JD MATON, PN AF LONDON, JF SHAWKER, TH DOPPMAN, JL FRUCHT, HH VINAYEK, R STARK, HA MILLER, LS MILLER, DL NORTON, JA JENSEN, RT GARDNER, JD MATON, PN TI ZOLLINGER-ELLISON SYNDROME - PROSPECTIVE ASSESSMENT OF ABDOMINAL US IN THE LOCALIZATION OF GASTRINOMAS SO RADIOLOGY LA English DT Article DE DUODENUM, NEOPLASMS; LIVER, US STUDIES; LIVER NEOPLASMS, SECONDARY; PANCREAS, NEOPLASMS; PANCREAS, US STUDIES ID ISLET CELL TUMORS; MANAGEMENT; ANGIOGRAPHY; CT; TOMOGRAPHY; METASTASES; RESECTION AB The ability of abdominal ultrasound (US) to help localize gastrinomas was prospectively studied in 79 patients with Zollinger-Ellison syndrome. The results were assessed by means of laparotomy, autopsy, or percutaneous liver biopsy. For hepatic gastrinoma, US had a sensitivity of 63% and a specificity of 100%, with a positive predictive value of 100% and a negative predictive value of 89%. US was slightly less sensitive for detecting gastrinoma in the liver than were computed tomography (CT) (66%) and selective angiography (78%). For detection of extrahepatic gastrinoma, US had a sensitivity of 30%, a specificity of 94%, a positive predictive value of 100%, and a negative predictive value of 25%. US enabled detection of tumor in eight cases not detected with CT and in four not detected with angiography. Specificity for extrahepatic gastrinoma was similar for all three modalities (89%-95%). CT and US were equally effective for the detection of extrahepatic gastrinoma, and angiography was significantly more effective than both US and CT (P < .01). The authors conclude that US, although of low sensitivity, remains useful as the initial imaging modality in patients with Zollinger-Ellison syndrome. C1 NIDDKD,DIGEST DIS BRANCH,BLDG 10,RM 9C-103,BETHESDA,MD 20892. NCI,SURG BRANCH,BETHESDA,MD 20892. NIH,WARREN MAGNUSON CLIN CTR,BETHESDA,MD 20892. NR 25 TC 44 Z9 45 U1 0 U2 0 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD MAR PY 1991 VL 178 IS 3 BP 763 EP 767 PG 5 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EY097 UT WOS:A1991EY09700033 PM 1994415 ER PT J AU WEINBERGER, M WU, T RUBIN, M GILL, VJ PIZZO, PA AF WEINBERGER, M WU, T RUBIN, M GILL, VJ PIZZO, PA TI LEPTOTRICHIA-BUCCALIS BACTEREMIA IN PATIENTS WITH CANCER - REPORT OF 4 CASES AND REVIEW SO REVIEWS OF INFECTIOUS DISEASES LA English DT Review ID NEUTROPENIC PATIENTS; INFECTIONS; EMERGENCE; THERAPY AB Leptotrichia buccalis, an anaerobic gram-negative rod, is part of the normal oral flora and has rarely been isolated from clinical material. We describe four patients with neutropenia and progressive malignancy who had symptomatic L. buccalis bacteremia, and we review an additional four cases from the literature. The mean age of the patients was 31 years (range, 7-73 years), with an equal number of males and females. The number of positive blood cultures in each case ranged from one to four (mean, two); these cultures became positive after 48-120 hours (median, 54 hours). All tested isolates were sensitive to the beta-lactam agents, clindamycin, tetracycline, and metronidazole; five of seven were sensitive to chloramphenicol; and not one was sensitive to the aminoglycosides, vancomycin, ciprofloxacin, or erythromycin. Seven patients had one or more possible portals of entry for bacteremia, including mucositis (four patients), mucositis plus esophageal lesions (two), and possible mucositis plus diverticulitis (one). L. buccalis should be considered a potential pathogen in neutropenic patients, especially when breaks in the mucosal barriers are present. C1 NCI,PEDIAT BRANCH,X13T,BLDG 10,ROOM 13N240,BETHESDA,MD 20892. NCI,DIV MICROBIOL,BETHESDA,MD 20892. NR 45 TC 27 Z9 29 U1 0 U2 3 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0162-0886 J9 REV INFECT DIS PD MAR-APR PY 1991 VL 13 IS 2 BP 201 EP 206 PG 6 WC Immunology; Microbiology SC Immunology; Microbiology GA FD047 UT WOS:A1991FD04700002 PM 2041949 ER PT J AU ROBBINS, JB CHU, C WATSON, DC SZU, SC DANIELS, EM LOWE, CU SCHNEERSON, R AF ROBBINS, JB CHU, C WATSON, DC SZU, SC DANIELS, EM LOWE, CU SCHNEERSON, R TI O-SPECIFIC SIDE-CHAIN TOXIN-PROTEIN CONJUGATES AS PARENTERAL VACCINES FOR THE PREVENTION OF SHIGELLOSIS AND RELATED DISEASES SO REVIEWS OF INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT WORKSHOP ON INVASIVE DIARRHEAS, SHIGELLOSIS, AND DYSENTERY CY DEC 07-09, 1988 CL MAHIDOL UNIV, SALAYA CAMPUS, BANGKOK, THAILAND SP HARVARD INST INT HLTH, JOHNS HOPKINS SCH HYGIENE & TROP MED, DEPT INT HLTH, NEW ENGLAND MED CTR, DIV GEOG MED & INFECTIOUS DIS HO MAHIDOL UNIV, SALAYA CAMPUS ID VI-CAPSULAR POLYSACCHARIDE; ENTEROINVASIVE ESCHERICHIA-COLI; TYPHOID-FEVER; SALMONELLA-TYPHI; SHIGA TOXIN; FLEXNERI; ANTIGEN; LIPOPOLYSACCHARIDE; ANTIBODIES; DYSENTERY AB Only indirect evidence has been cited to document that lipopolysaccharide-mediated virulence at the bacterial level and serum antibodies to the O-specific side chain of the lipopolysaccharide molecule may prevent shigellosis. Our proposed use of the B subunit of Shiga toxin as a carrier protein is based upon evidence (even more indirect) that serum antitoxin may reduce the severity of dysentery and diarrhea. Because animal models of diseases may provide information inapplicable to the prediction of vaccine-induced protective immunity, we suggest that clinical trials in the population at risk should be started after successful completion of the safety and immunogenicity phases of vaccine development in laboratory animals and in the target population. Clinical studies of shigella vaccines are difficult because of the many causes of dysentery in a population with a high rate of intestinal disease. RP ROBBINS, JB (reprint author), NICHHD,DEV & MOLEC IMMUN LAB,BLDG 6,ROOM 145,BETHESDA,MD 20892, USA. NR 44 TC 12 Z9 12 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0162-0886 J9 REV INFECT DIS PD MAR-APR PY 1991 VL 13 SU 4 BP S362 EP S365 PG 4 WC Immunology; Microbiology SC Immunology; Microbiology GA FD059 UT WOS:A1991FD05900026 PM 2047664 ER PT J AU CARR, GJ PORTIER, CJ AF CARR, GJ PORTIER, CJ TI AN EVALUATION OF THE RAI AND VAN RYZIN DOSE - RESPONSE MODEL IN TERATOLOGY SO RISK ANALYSIS LA English DT Article DE REPRODUCTIVE TOXICOLOGY; TERATOLOGY; DOSE-RESPONSE MODELS; LITTER EFFECT; LITTER SIZE; LOGISTIC MODEL C1 NIEHS,STAT & BIOMATH BRANCH,RES TRIANGLE PK,NC 27709. RP CARR, GJ (reprint author), MARION MERRELL DOW,DEPT BIOSTAT,2110 E GALBRAITH RD,CINCINNATI,OH 45215, USA. RI Portier, Christopher/A-3160-2010 OI Portier, Christopher/0000-0002-0954-0279 NR 71 TC 11 Z9 11 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0272-4332 J9 RISK ANAL JI Risk Anal. PD MAR PY 1991 VL 11 IS 1 BP 111 EP 120 DI 10.1111/j.1539-6924.1991.tb00581.x PG 10 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA FD081 UT WOS:A1991FD08100026 PM 2038612 ER PT J AU BIASSONI, R FERRINI, S PRIGIONE, I PELAK, VS SEKALY, RP LONG, EO AF BIASSONI, R FERRINI, S PRIGIONE, I PELAK, VS SEKALY, RP LONG, EO TI ACTIVATED CD3-CD16+ NATURAL-KILLER-CELLS EXPRESS A SUBSET OF THE LYMPHOKINE GENES INDUCED IN ACTIVATED ALPHA-BETA+ AND GAMMA-DELTA+ T-CELLS SO SCANDINAVIAN JOURNAL OF IMMUNOLOGY LA English DT Article ID TUMOR NECROSIS FACTOR; CYTO-TOXIC CELLS; CD3+WT31 LYMPHOCYTES; FUNCTIONAL-ANALYSIS; COMPLEMENTARY-DNA; RECEPTOR; CLONES; CDNA; INTERLEUKIN-2; CLONING AB In this study we analysed the potential of highly purified polyclonal TcR alpha-beta+, TcR gamma-delta+ and CD3-NK cells, to produce lymphokines in response to mitogenic stimulation. RNA hybridizations were performed to detect with high sensitivity the induction of multiple lymphokine genes. Upon stimulation with lectin and phorbol ester TcR gamma-delta+ lymphocytes expressed the same set of lymphokine genes as the TcR alpha-beta+ lymphocytes, which included IL-2, -3, -4, -5, GM-CSF, TNF-alpha and beta, IFN-gamma. In contrast, a more limited set of lymphokine genes (GM-CSF, TNF-alpha and beta, IFN-gamma) was induced in activated CD3- NK cells, thus indicating that this subpopulation of cells may display different regulatory functions, with respect to CD3+ T lymphocytes. C1 NIAID,IMMUNOGENET LAB,BETHESDA,MD 20892. RP BIASSONI, R (reprint author), IST NAZL RIC SUL CANC,FARMACOL LAB,VIALE BENEDETTO XV 10,I-16132 GENOA,ITALY. RI Long, Eric/G-5475-2011 OI Long, Eric/0000-0002-7793-3728 NR 33 TC 21 Z9 21 U1 0 U2 1 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0300-9475 J9 SCAND J IMMUNOL JI Scand. J. Immunol. PD MAR PY 1991 VL 33 IS 3 BP 247 EP 252 DI 10.1111/j.1365-3083.1991.tb01769.x PG 6 WC Immunology SC Immunology GA FA366 UT WOS:A1991FA36600002 PM 1707180 ER PT J AU SPRING, KR AF SPRING, KR TI DETECTORS FOR FLUORESCENCE MICROSCOPY SO SCANNING MICROSCOPY LA English DT Article DE LOW LIGHT IMAGING; IMAGE INTENSIFIERS; PHOTOMULTIPLIERS; RESOLUTION; RESPONSE SPEED; INTENSIFIED VIDEO CAMERAS ID DIGITAL IMAGING MICROSCOPY AB The low light levels originating from living cells viewed in the fluorescence microscope place significant limitations on the spatial and temporal resolution which can be achieved. The development of intensified video cameras has enabled the detection, visualization and measurement of these low level signals. The performance characteristics of popular intensified video cameras are compared and guidelines are given for the selection of the appropriate detector for various experimental requirements. Intensified or cooled CCD cameras appear to be the most suitable device for quantitative imaging at low light levels in fluorescence microscopy. RP SPRING, KR (reprint author), NHLBI,BLDG 10,ROOM 6N307,BETHESDA,MD 20892, USA. NR 22 TC 4 Z9 4 U1 0 U2 2 PU SCANNING MICROSCOPY INT PI CHICAGO PA PO BOX 66507, AMF O'HARE, CHICAGO, IL 60666 SN 0891-7035 J9 SCANNING MICROSCOPY JI Scanning Microsc. PD MAR PY 1991 VL 5 IS 1 BP 63 EP 69 PG 7 WC Microscopy SC Microscopy GA FH432 UT WOS:A1991FH43200007 PM 2052930 ER PT J AU LO, SC TSAI, S BENISH, JR SHIH, JWK WEAR, DJ WONG, DM AF LO, SC TSAI, S BENISH, JR SHIH, JWK WEAR, DJ WONG, DM TI ENHANCEMENT OF HIV-1 CYTOCIDAL EFFECTS IN CD4+ LYMPHOCYTES BY THE AIDS-ASSOCIATED MYCOPLASMA SO SCIENCE LA English DT Article ID INFECTIOUS AGENT VLIA; HTLV-III/LAV ENVELOPE; CELL-FUSION; VIRUS; IDENTIFICATION; INCOGNITUS; INDUCTION AB Coinfection with Mycoplasma fermentans (incognitus strain) enhances the ability of human immunodeficiency virus type-1 (HIV-1) to induce cytopathic effects on human T lymphocytes in vitro. Syncytium formation of HIV-infected T cells was essentially eliminated in the presence of M. fermentans (incognitus strain), despite prominent cell death. However, replication and production of HIV-1 particles continued during the coinfection. Furthermore, the supernatant from cultures coinfected with HIV-1 and the mycoplasma contained a factor that inhibited the standard reverse transcriptase enzyme assay. The modification of the biological properties of HIV-1 by coinfection with mycoplasma may be involved in the pathogenesis of acquired immunodeficiency syndrome (AIDS). C1 BIOTECH RES LABS INC,ROCKVILLE,MD 20852. NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT TRANSFUS MED,BETHESDA,MD 20892. RP LO, SC (reprint author), ARMED FORCES INST PATHOL,DEPT INFECT & PARASIT DIS PATHOL,AMER REGISTRY PATHOL,WASHINGTON,DC 20306, USA. NR 20 TC 141 Z9 143 U1 0 U2 3 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 1 PY 1991 VL 251 IS 4997 BP 1074 EP 1076 DI 10.1126/science.1705362 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EZ665 UT WOS:A1991EZ66500051 PM 1705362 ER PT J AU JOZIASSE, DH SHAPER, NL SHAPER, JH KOZAK, CA AF JOZIASSE, DH SHAPER, NL SHAPER, JH KOZAK, CA TI GENE FOR MURINE ALPHA-1-] 3-GALACTOSYLTRANSFERASE IS LOCATED IN THE CENTROMERIC REGION OF CHROMOSOME-2 SO SOMATIC CELL AND MOLECULAR GENETICS LA English DT Note ID GALACTOSYLTRANSFERASE; IDENTIFICATION; EXPRESSION; SEQUENCES; GALACTOSE; CLONE; DNA AB The gene for alpha-1 --> 3-galactosyltransferase, termed Ggta-1, was mapped to mouse chromosome 2 by Southern blot analysis of Chinese hamster x mouse somatic cell hybrids. Using an intersubspecies back-cross, this locus was positioned to the centromeric region on this chromosome, near the Hc locus. C1 JOHNS HOPKINS UNIV,SCH MED,CTR ONCOL,CELL STRUCT & FUNCT LAB,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOLEC SCI,BALTIMORE,MD 21205. NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. RP JOZIASSE, DH (reprint author), FREE UNIV AMSTERDAM,DEPT MED CHEM,1007 MC AMSTERDAM,NETHERLANDS. FU NIGMS NIH HHS [GM-38310, GM-41015] NR 23 TC 7 Z9 7 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0740-7750 J9 SOMAT CELL MOLEC GEN JI Somat.Cell Mol.Genet. PD MAR PY 1991 VL 17 IS 2 BP 201 EP 205 DI 10.1007/BF01232977 PG 5 WC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity GA FD594 UT WOS:A1991FD59400009 PM 1901427 ER PT J AU GIORDANO, JM LEAVITT, RY HOFFMAN, G FAUCI, AS AF GIORDANO, JM LEAVITT, RY HOFFMAN, G FAUCI, AS TI EXPERIENCE WITH SURGICAL-TREATMENT OF TAKAYASUS DISEASE SO SURGERY LA English DT Article ID AORTITIS SYNDROME; ARTERITIS AB We reviewed 28 patients with Takayasu's disease to determine the incidence of stroke and its relationship to the involvement of the thoracic aortic arch and its branches. We describe surgical experiences with 10 of the 28 patients who required 21 vascular surgical procedures for critical thoracic aortic arch arterial stenoses, upper and lower extremity ischemia, and renal artery stenoses. Four of the 28 patients initially had a stroke caused by occlusion of one or more thoracic aortic arch arteries. Six of the 10 patients underwent 7 bypass procedures for critical thoracic arch stenoses. All have remained free of stroke for 5 or more years. Four patients had five anastomotic stenoses or graft occlusions in late follow-up; the development of these stenoses did not relate to disease activity at the time of the operative procedure. All bypass grafts originating from the subclavian axillary artery developed anastomotic stenoses; no anastomotic stenoses occurred in bypass grafts originating from the ascending aorta. In contrast to other reports, no anastomotic false aneurysms occurred. Occlusions of major aortic arch arteries in Takayasu's disease cause stroke. Bypass of critically stenoses aortic arch arteries protects against stroke and is best performed with grafts originating from the ascending aorta. Anastomotic stenoses but not anastomotic aneurysms are common. This study suggests that aggressive surgical treatment can be performed with good results. C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. RP GIORDANO, JM (reprint author), GEORGE WASHINGTON UNIV,MED CTR,DEPT SURG,2150 PENN AVE NW,WASHINGTON,DC 20037, USA. NR 13 TC 68 Z9 70 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0039-6060 J9 SURGERY JI Surgery PD MAR PY 1991 VL 109 IS 3 BP 252 EP 258 PN 1 PG 7 WC Surgery SC Surgery GA EZ891 UT WOS:A1991EZ89100004 PM 1672048 ER PT J AU LOTZE, MT AF LOTZE, MT TI CONCERNING REPEAT HEPATIC RESECTIONS FOR COLORECTAL METASTASES SO SURGERY LA English DT Letter RP LOTZE, MT (reprint author), NCI,SURG BRANCH,TUMOR IMMUNOL SECT,BETHESDA,MD 20892, USA. NR 4 TC 0 Z9 0 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0039-6060 J9 SURGERY JI Surgery PD MAR PY 1991 VL 109 IS 3 BP 347 EP 348 PN 1 PG 2 WC Surgery SC Surgery GA EZ891 UT WOS:A1991EZ89100026 PM 2000570 ER PT J AU COUTUREHAWS, L HARRIS, MW LOCKHART, AC BIRNBAUM, LS AF COUTUREHAWS, L HARRIS, MW LOCKHART, AC BIRNBAUM, LS TI EVALUATION OF THE PERSISTENCE OF HYDRONEPHROSIS INDUCED IN MICE FOLLOWING INUTERO AND OR LACTATIONAL EXPOSURE TO 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID POLYCHLORODIBENZO-PARA-DIOXINS; HUMAN-MILK; CHLORINATED DIOXINS; C57BL/6N MICE; NEONATAL RATS; FLY-ASH; DIBENZOFURANS; TOXICITY; PCDDS; PCDFS C1 NIEHS,EXPTL TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. UNIV N CAROLINA,CURRICULUM TOXICOL,CHAPEL HILL,NC 27599. NIEHS,SYST TOXIC BRANCH,RES TRIANGLE PK,NC 27709. COMP SCI CORP,RES TRIANGLE PK,NC 27709. NR 37 TC 21 Z9 21 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD MAR 1 PY 1991 VL 107 IS 3 BP 402 EP 412 DI 10.1016/0041-008X(91)90304-W PG 11 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EZ947 UT WOS:A1991EZ94700002 PM 2000631 ER PT J AU COUTUREHAWS, L HARRIS, MW MCDONALD, MM LOCKHART, AC BIRNBAUM, LS AF COUTUREHAWS, L HARRIS, MW MCDONALD, MM LOCKHART, AC BIRNBAUM, LS TI HYDRONEPHROSIS IN MICE EXPOSED TO TCDD-CONTAMINATED BREAST-MILK - IDENTIFICATION OF THE PEAK PERIOD OF SENSITIVITY AND ASSESSMENT OF POTENTIAL RECOVERY SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID CHLORINATED DIOXINS; 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN; DIBENZOFURANS; TOXICITY; ACETAZOLAMIDE; FURANS; PCDDS; PCDFS; FATE; RATS C1 US EPA,DIV ENVIRONM TOXICOL,RES TRIANGLE PK,NC 27711. COMP SCI CORP,RES TRIANGLE PK,NC 27709. NIEHS,EXPTL TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. NIEHS,CHEM CARCINOGENESIS BRANCH,RES TRIANGLE PK,NC 27709. UNIV N CAROLINA,CURRICULUM TOXICOL,CHAPEL HILL,NC 27599. NIEHS,SYST TOX BRANCH,RES TRIANGLE PK,NC 27709. NR 49 TC 27 Z9 28 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD MAR 1 PY 1991 VL 107 IS 3 BP 413 EP 428 DI 10.1016/0041-008X(91)90305-X PG 16 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EZ947 UT WOS:A1991EZ94700003 PM 2000632 ER PT J AU BOND, JA BECHTOLD, WE BIRNBAUM, LS DAHL, AR MEDINSKY, MA SUN, JD HENDERSON, RF AF BOND, JA BECHTOLD, WE BIRNBAUM, LS DAHL, AR MEDINSKY, MA SUN, JD HENDERSON, RF TI DISPOSITION OF INHALED ISOPRENE IN B6C3F1 MICE SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID C-14 METHYL BROMIDE; RATS; BUTADIENE; EXPOSURE; GAS C1 LOVELACE BIOMED & ENVIRONM RES INST,INHALAT TOXICOL RES INST,ALBUQUERQUE,NM 87185. NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709. FU NIEHS NIH HHS [222-Y02-ES-20092] NR 23 TC 11 Z9 11 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD MAR 1 PY 1991 VL 107 IS 3 BP 494 EP 503 DI 10.1016/0041-008X(91)90312-3 PG 10 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EZ947 UT WOS:A1991EZ94700010 PM 2000636 ER PT J AU ROSENTHAL, GJ CORSINI, E CRAIG, WA COMMENT, CE LUSTER, MI AF ROSENTHAL, GJ CORSINI, E CRAIG, WA COMMENT, CE LUSTER, MI TI PENTAMIDINE - AN INHIBITOR OF INTERLEUKIN-1 THAT ACTS VIA A POSTTRANSLATIONAL EVENT SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Note ID MEMBRANE-ASSOCIATED INTERLEUKIN-1; IL-1; PRECURSOR; LOCALIZATION; IL-1-ALPHA; EXPRESSION; SECRETION; MONOCYTES; RELEASE; TRYPSIN C1 UNIV MILAN,INST PHARMACOL SCI,I-20122 MILAN,ITALY. RP ROSENTHAL, GJ (reprint author), NIEHS,DIV TOXICOL RES & TESTING,RES TRIANGLE PK,NC 27709, USA. RI Corsini, Emanuela/B-5602-2011 NR 19 TC 22 Z9 23 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD MAR 1 PY 1991 VL 107 IS 3 BP 555 EP 561 DI 10.1016/0041-008X(91)90318-9 PG 7 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EZ947 UT WOS:A1991EZ94700016 PM 2000641 ER PT J AU CUNNINGHAM, ML FOLEY, J MARONPOT, RR MATTHEWS, HB AF CUNNINGHAM, ML FOLEY, J MARONPOT, RR MATTHEWS, HB TI CORRELATION OF HEPATOCELLULAR PROLIFERATION WITH HEPATOCARCINOGENICITY INDUCED BY THE MUTAGENIC NONCARCINOGEN - CARCINOGEN PAIR - 2,6-DIAMINOTOLUENE AND 2,4-DIAMINOTOLUENE SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Note ID <4-CHLORO-6-(2,3-XYLIDINO)-2-PYRIMIDINYLTHIO>ACETIC ACID WY-14,643; PEROXISOME PROLIFERATOR; LIVER CARCINOGENESIS; CELL-PROLIFERATION; DNA-SYNTHESIS; MOUSE-LIVER; RAT; INITIATION; DI(2-ETHYLHEXYL)PHTHALATE; IDENTIFICATION C1 NIEHS,EXPTL CARCINOGENESIS & MUTAGENESIS BRANCH,RES TRIANGLE PK,NC 27709. RP CUNNINGHAM, ML (reprint author), NIEHS,EXPTL TOXICOL BRANCH,RES TRIANGLE PK,NC 27709, USA. NR 27 TC 65 Z9 65 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD MAR 1 PY 1991 VL 107 IS 3 BP 562 EP 567 DI 10.1016/0041-008X(91)90319-A PG 6 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EZ947 UT WOS:A1991EZ94700017 PM 2000642 ER PT J AU JALIL, F ZAMAN, S CARLSSON, B GLASS, RI KAPIKIAN, AZ MELLANDER, L HANSON, LA AF JALIL, F ZAMAN, S CARLSSON, B GLASS, RI KAPIKIAN, AZ MELLANDER, L HANSON, LA TI IMMUNOGENICITY AND REACTOGENICITY OF RHESUS ROTAVIRUS VACCINE GIVEN IN COMBINATION WITH ORAL OR INACTIVATED POLIOVIRUS VACCINES AND DIPHTHERIA-TETANUS-PERTUSSIS VACCINE SO TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE LA English DT Article ID YOUNG-CHILDREN; INFANTS; LIVE; GASTROENTERITIS; RIT-4237; IMMUNIZATION; ANTIBODIES; DIARRHEA; TRIAL AB Immunogenicity and reactogenicity of the oral rhesus rotavirus vaccine (RRV) were assessed among 72 infants (6 weeks old) in Lahore, Pakistan, from August to December 1985. Special emphasis was placed on the possible interaction or interference caused by giving RRV at the time infants received their first polio immunization. RRV was given to the infants at the same time as diphtheria-tetanus-pertussis (DTP), oral poliovirus vaccine (OPV), or inactivated poliovirus vaccine (IPV). The immune response to RRV was assessed by plaque-reduction neutralization 3 weeks after immunization and serum immunoglobulin (Ig) G and IgA antibody levels to poliovirus type 1 were tested by enzyme-linked immunosorbent assay (ELISA) after polio immunizations. Of the infants in the group given RRV with OPV, 50% had a two- to four-fold rise in neutralization titre against rotavirus, compared with 22% in the group given RRV with DTP and 20% in the group given RRV and IPV (P < 0.05). Interference by live oral polio vaccination in the response to RRV seems unlikely. We observed no significant difference in rates of seroconversion of IgG antibodies to poliovirus type 1 among infants aged 18 and 21 weeks who received RRV and OPV (81%), RRV with delayed OPV (67%), or RRV and IPV (59%). Administration of RRV was safe and was not associated with adverse reactions in the 6 weeks old infants. The low rate of seroconversion to rotavirus suggests that a more antigen-rich vaccine or multiple doses of the same vaccine might produce a better immune response. C1 GOTHENBURG UNIV,DEPT CLIN IMMUNOL,S-41346 GOTHENBURG,SWEDEN. GOTHENBURG UNIV,DEPT PAEDIAT,S-41346 GOTHENBURG,SWEDEN. CTR DIS CONTROL,VIRAL GASTROENTERITIS UNIT,ATLANTA,GA 30333. NIAID,INFECT DIS LAB,BETHESDA,MD 20892. RP JALIL, F (reprint author), KING EDWARD MED COLL,DEPT SOCIAL & PREVENT PAEDIAT,LAHORE,PAKISTAN. NR 33 TC 11 Z9 11 U1 1 U2 1 PU ROYAL SOC TROPICAL MEDICINE PI LONDON PA MANSON HOUSE 26 PORTLAND PLACE, LONDON, ENGLAND W1N 4EY SN 0035-9203 J9 T ROY SOC TROP MED H JI Trans. Roy. Soc. Trop. Med. Hyg. PD MAR-APR PY 1991 VL 85 IS 2 BP 292 EP 296 DI 10.1016/0035-9203(91)90061-3 PG 5 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA FN691 UT WOS:A1991FN69100046 PM 1653474 ER PT J AU DEPALMA, L CRISS, VR SULLIVAN, MT LEITMAN, SF WILLIAMS, AE LUBAN, NLC AF DEPALMA, L CRISS, VR SULLIVAN, MT LEITMAN, SF WILLIAMS, AE LUBAN, NLC TI DETECTION OF CYTOMEGALOVIRUS ANTIBODY IN PLATELET CONCENTRATES BY FLUORESCENCE IMMUNOASSAY AND LATEX AGGLUTINATION SO TRANSFUSION LA English DT Article ID VIRUS INFECTIONS; BLOOD-DONORS; RISK-FACTORS; TRANSPLANTATION; RECIPIENTS; CMV AB A passive latex agglutination (PLA) test for cytomegalovirus (CMV) antibody detection has been shown to be an acceptable method of screening both donor sera and plasma from units of red cells and platelets stored in CPDA-1. However, most plateletpheresis concentrates are collected in ACD, and CMV antibody testing of ACD-stored products has not been systematically evaluated by PLA. Sera and ACD-stored platelet concentrate bag segments from 104 donors were tested by PLA and by a solid-phase fluorescence immunoassay (FIAX) as a reference standard for CMV-IgM and CMV-IgG antibodies. Sera were stored at both 4 and 22-degrees-C and were tested on Days 1 and 5 of storage; segments were tested daily for 5 days. Of 63 donor samples (61%) that tested negative for CMV-IgG by FIAX, there were two false-positive results in bag segments by PLA testing, one on Day 1 and the other on Day 2 of storage. PLA testing was consistently positive in sera and segments in the 40 donors (38%) who tested positive for CMV-IgG by FIAX. Potential false-negative PLA results occurred in five bag segments derived from one donor whose serum gave equivocal CMV-IgG results on FIAX. The sensitivity and specificity of the PLA assay were 100 percent for donor sera tested at both 4 and 22-degrees-C and 91.5 and 98.4 percent, respectively, for platelet bag segment tests. Although no donors positive for CMV-IgM were identified, 15 (14.4%) had equivocal IgM anti-CMV test results. The PLA positivity of these samples correlated solely with the presence or absence of IgG as detected by FIAX. A total of 648 PLA tests were performed on donor sera and on platelet bag segments. Only two false-positive and five false-negative results were observed. PLA testing for CMV antibody in donor sera and in ACD-stored platelet concentrates is accurate and sensitive for up to 5 days. Segments may be removed from platelet bags for testing throughout the 5-day shelf life of plateletpheresis components and will still provide accurate CMV testing results. C1 GEORGE WASHINGTON UNIV,SCH MED,WASHINGTON,DC 20052. NIH,ROCKVILLE,MD. AMER RED CROSS,JEROME H HOLLAND LAB,ROCKVILLE,MD. RP DEPALMA, L (reprint author), CHILDRENS NATL MED CTR,BLOOD BANK HEMATOL,111 MICHIGAN AVE NW,WASHINGTON,DC 20010, USA. NR 10 TC 3 Z9 3 U1 0 U2 0 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 SN 0041-1132 J9 TRANSFUSION JI Transfusion PD MAR-APR PY 1991 VL 31 IS 3 BP 245 EP 248 DI 10.1046/j.1537-2995.1991.31391165174.x PG 4 WC Hematology SC Hematology GA FB961 UT WOS:A1991FB96100011 PM 1848376 ER PT J AU YOUNG, JB WINDSOR, NT SMART, FW KLEIMAN, NS WEILBAECHER, DG NOON, GP NELSON, DL LAWRENCE, EC AF YOUNG, JB WINDSOR, NT SMART, FW KLEIMAN, NS WEILBAECHER, DG NOON, GP NELSON, DL LAWRENCE, EC TI INABILITY OF ISOLATED SOLUBLE INTERLEUKIN-2 RECEPTOR LEVELS TO PREDICT BIOPSY REJECTION SCORES AFTER HEART-TRANSPLANTATION SO TRANSPLANTATION LA English DT Article ID RENAL-ALLOGRAFT RECIPIENTS; SERUM; SYSTEM; CELLS AB Successful cardiac transplantation requires suppression of rejection, and endomyocardial biopsy is generally used to quantify this and guide immunotherapy. Biopsy, however, is an invasive, costly, cardiac catheterization with repetition limited. Since rejection requires lymphocyte activation, an alternative method of assessing rejection dynamics might be ELISA determination of soluble interleukin-2 receptor (sIL-2R) levels since induction of the interleukin-2 ligand and its receptor is required. Reports suggest that sIL-2R levels rise during kidney, liver, and heart-lung allograft rejection, and heart recipients have an adverse prognosis if sIL-2R is elevated postoperatively. It is unclear, however, if serial measurements or single determinations are sufficient or if change from a baseline assessment is important. The purpose of this study was to determine if an isolated sIL-2R level after heart transplant predicted endomyocardial biopsy score at that moment. To do this, we prospectively followed 60 consecutive patients after orthotopic heart transplant and correlated 479 endomyocardial biopsy scores (McAllister scale 0-10) with matched sIL-2R levels. Regression analysis demonstrated minimal relationship between sIL-2R level and biopsy score (r = .11, r2 = .01, P = .009). When the maximum sIL-2R level for each individual patient was compared with the matched biopsy score, regression analysis revealed r = .04, r2 = .001, P = .8. Likewise, when all biopsy scores and sIL-2R levels for each patient were meaned, analysis showed r = .14, r2 = .02, P = .26. Thus in heart transplant patients, there is poor correlation between an isolated biopsy score and matched sIL-2R level. However, when mean +/- SEM sIL-2R was determined for severe rejection (score 7-10) and compared with sIL-2R for all other grades, it was significantly higher (1600 +/- 257 vs. 423 +/- 57 U/ml; P = .012). Still, the sensitivity, specificity, and predictive value of an sIL-2R level above 1000 U/ml predicting severe rejection was only 52%, 63%, and 8%. It would be difficult, therefore, to use a single sIL-2R determination after heart transplant to foretell the endomyocadial biopsy score. Serial measurements or quantification of a change in sIL-2R level from baseline might be more predictive of rejection severity. C1 METHODIST HOSP,CTR MULTI ORGAN TRANSPLANT,HOUSTON,TX 77030. BAYLOR UNIV,HOUSTON,TX 77030. NIH,BETHESDA,MD 20892. FU NCRR NIH HHS [RR-0035] NR 26 TC 17 Z9 17 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD MAR PY 1991 VL 51 IS 3 BP 636 EP 641 DI 10.1097/00007890-199103000-00018 PG 6 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA FC649 UT WOS:A1991FC64900018 PM 2006520 ER PT J AU MURPHY, BR PRINCE, GA COLLINS, PL HILDRETH, SW PARADISO, PR AF MURPHY, BR PRINCE, GA COLLINS, PL HILDRETH, SW PARADISO, PR TI EFFECT OF PASSIVE ANTIBODY ON THE IMMUNE-RESPONSE OF COTTON RATS TO PURIFIED F-GLYCOPROTEINS AND G-GLYCOPROTEINS OF RESPIRATORY SYNCYTIAL VIRUS (RSV) SO VACCINE LA English DT Article DE RESPIRATORY SYNCYTIAL VIRUS; SUBUNIT VACCINE; ANTIBODY-MEDIATED IMMUNE SUPPRESSION; VACCINIA RSV RECOMBINANTS ID VACCINIA VIRUS; FUSION F; INFECTION; IMMUNIZATION; SERUM; CHILDREN; INFANTS AB The effect of passively transferred RSV immune serum on the antibody response to a single dose of purified RSV fusion (F) and large (G) glycoproteins was studied in cotton rats. Passively transferred antibody that achieved serum antibody levels similar to those seen in newborn human infants resulted in a seven- to eightfold suppression of the neutralizing antibody response of cotton rats to low doses of purified F and G glycoproteins (0.2-1.7-mu-g) and a twofold suppression to higher doses of these antigens (5-15-mu-g). This suppression of the antibody response was accompanied by a reduction in the protective efficacy of the F and G purified glycoprotein vaccine. These results suggest that parenteral immunization with RSV antigens could be less immunogenic in seropositive human infants, but that this suppressive effect might be partially overcome with increased antigen dose. C1 JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,BALTIMORE,MD 21205. PRAXIS BIOL,ROCHESTER,NY 14623. RP MURPHY, BR (reprint author), NIAID,INFECT DIS LAB,BETHESDA,MD 20892, USA. NR 13 TC 27 Z9 27 U1 0 U2 1 PU BUTTERWORTH-HEINEMANN LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0264-410X J9 VACCINE JI Vaccine PD MAR PY 1991 VL 9 IS 3 BP 185 EP 189 DI 10.1016/0264-410X(91)90151-U PG 5 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA EZ099 UT WOS:A1991EZ09900006 PM 2042390 ER PT J AU ENGLUND, G HOGGAN, MD THEODORE, TS MARTIN, MA AF ENGLUND, G HOGGAN, MD THEODORE, TS MARTIN, MA TI A NOVEL HIV-1 ISOLATE CONTAINING ALTERATIONS AFFECTING THE NF-KAPPA-B ELEMENT SO VIROLOGY LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; LONG TERMINAL REPEAT; MURINE LEUKEMIA VIRUSES; HTLV-III/LAV; TRANSCRIPTION FACTOR; AIDS RETROVIRUS; TUMOR PROMOTER; T-CELLS; ACTIVATION; ENHANCER RP ENGLUND, G (reprint author), NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892, USA. NR 36 TC 39 Z9 39 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD MAR PY 1991 VL 181 IS 1 BP 150 EP 157 DI 10.1016/0042-6822(91)90479-U PG 8 WC Virology SC Virology GA EW942 UT WOS:A1991EW94200016 PM 1994572 ER PT J AU JEANG, KT CHIU, R SANTOS, E KIM, SJ AF JEANG, KT CHIU, R SANTOS, E KIM, SJ TI INDUCTION OF THE HTLV-I LTR BY JUN OCCURS THROUGH THE TAX-RESPONSIVE 21-BP ELEMENTS SO VIROLOGY LA English DT Article ID CELL LEUKEMIA-VIRUS; LONG TERMINAL REPEAT; TRANSCRIPTION FACTOR AP-1; PROTO-ONCOGENE; CYCLIC-AMP; V-JUN; INDUCIBLE ENHANCER; GENE-EXPRESSION; TUMOR-PROMOTER; C-JUN C1 NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. UNIV CALIF LOS ANGELES,DEPT SURG,LOS ANGELES,CA 90024. RP JEANG, KT (reprint author), NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892, USA. RI Jeang, Kuan-Teh/A-2424-2008 NR 62 TC 58 Z9 58 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD MAR PY 1991 VL 181 IS 1 BP 218 EP 227 DI 10.1016/0042-6822(91)90487-V PG 10 WC Virology SC Virology GA EW942 UT WOS:A1991EW94200024 PM 1899734 ER PT J AU VILLA, LL SCHLEGEL, R AF VILLA, LL SCHLEGEL, R TI DIFFERENCES IN TRANSFORMATION ACTIVITY BETWEEN HPV-18 AND HPV-16 MAP TO THE VIRAL LCR-E6-E7 REGION SO VIROLOGY LA English DT Note ID HUMAN PAPILLOMAVIRUS TYPE-16; HUMAN KERATINOCYTES; CERVICAL-CARCINOMA; DNA; CANCER; TRANSFECTION; SUFFICIENT; INVITRO C1 LUDWIG INST CANC RES,SAO PAULO,BRAZIL. NCI,TUMOR VIRUS BIOL LAB,BETHESDA,MD 20892. GEORGETOWN UNIV,DEPT PATHOL,WASHINGTON,DC 20057. NR 20 TC 56 Z9 59 U1 1 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD MAR PY 1991 VL 181 IS 1 BP 374 EP 377 PG 4 WC Virology SC Virology GA EW942 UT WOS:A1991EW94200044 PM 1847260 ER PT J AU ZWIEBEL, JA FREEMAN, SM NEWMAN, K DICHEK, D RYAN, US ANDERSON, WF AF ZWIEBEL, JA FREEMAN, SM NEWMAN, K DICHEK, D RYAN, US ANDERSON, WF TI DRUG DELIVERY BY GENETICALLY ENGINEERED CELL IMPLANTS SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID MEDIATED GENE-TRANSFER; HUMAN ADENOSINE-DEAMINASE; HEMATOPOIETIC STEM-CELLS; ENDOTHELIAL-CELLS; HUMAN GLUCOCEREBROSIDASE; RETROVIRAL VECTORS; SKIN FIBROBLASTS; EXPRESSION; THERAPY; INVIVO C1 UNIV ROCHESTER,DEPT MED,ROCHESTER,NY 14642. NCI,BETHESDA,MD 20892. CHILDRENS NATL MED CTR,WASHINGTON,DC 20001. NHLBI,MOLEC HEMATOL BRANCH,BETHESDA,MD 20892. MONSANTO CO,ST LOUIS,MO 63167. WASHINGTON UNIV,ST LOUIS,MO 63130. RP ZWIEBEL, JA (reprint author), GEORGETOWN UNIV,MED CTR,VINCENT T LOMBARDI CANC RES CTR,3800 RESERVOIR RD NW,WASHINGTON,DC 20007, USA. NR 33 TC 8 Z9 8 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 SN 0077-8923 J9 ANN NY ACAD SCI JI Ann. N.Y. Acad. Sci. PD FEB 28 PY 1991 VL 618 BP 394 EP 404 DI 10.1111/j.1749-6632.1991.tb27259.x PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FQ403 UT WOS:A1991FQ40300028 PM 2006798 ER PT J AU FERNANDEZ, MT CASTRONOVO, V RAO, CN SOBEL, ME AF FERNANDEZ, MT CASTRONOVO, V RAO, CN SOBEL, ME TI THE HIGH-AFFINITY MURINE LAMININ RECEPTOR IS A MEMBER OF A MULTICOPY GENE FAMILY SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID NUCLEOTIDE-SEQUENCE; BINDING-PROTEIN; TUMOR-CELLS; MESSENGER-RNA; METASTASIS; CONTAINS; LINES C1 NCI,PATHOL LAB,TUMOR INVAS & METASTASIS SECT,BETHESDA,MD 20892. RI Fernandez-Sanchez, Maria Teresa/Q-1673-2015 OI Fernandez-Sanchez, Maria Teresa/0000-0003-4800-9517 NR 23 TC 21 Z9 21 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD FEB 28 PY 1991 VL 175 IS 1 BP 84 EP 90 DI 10.1016/S0006-291X(05)81203-3 PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EZ636 UT WOS:A1991EZ63600013 PM 1825600 ER PT J AU LIN, KH CHENG, SY AF LIN, KH CHENG, SY TI ZN+2 INDUCES REVERSIBLE CROSS-LINKING OF HUMAN PLACENTAL THYROID-HORMONE NUCLEAR RECEPTOR WITH NO EFFECT ON HORMONE BINDING SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID CELLS; LIVER C1 NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 11 TC 10 Z9 10 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD FEB 28 PY 1991 VL 175 IS 1 BP 250 EP 255 DI 10.1016/S0006-291X(05)81227-6 PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EZ636 UT WOS:A1991EZ63600037 PM 1847806 ER PT J AU GUO, HG CHERMANN, JC WATERS, D HALL, L LOUIE, A GALLO, RC STREICHER, H REITZ, MS POPOVIC, M BLATTNER, W AF GUO, HG CHERMANN, JC WATERS, D HALL, L LOUIE, A GALLO, RC STREICHER, H REITZ, MS POPOVIC, M BLATTNER, W TI SEQUENCE-ANALYSIS OF ORIGINAL HIV-1 SO NATURE LA English DT Letter ID RETROVIRUSES HTLV-III; IMMUNE-DEFICIENCY SYNDROME; NUCLEOTIDE-SEQUENCE; AIDS VIRUS; AFRICAN PATIENTS; RISK; ANTIBODIES; LAV C1 INSERM,F-13258 MARSEILLE 09,FRANCE. PROGRAM RESOURCES INC,FCRDC,FREDERICK,MD 21701. NCI,TUMOR CELL BIOL LAB,BETHESDA,MD 20892. NCI,VIRAL EPIDEMIOL SECT,BETHESDA,MD 20892. RP GUO, HG (reprint author), LOFSTRAND LABS LTD,GAITHERSBURG,MD 20877, USA. NR 17 TC 18 Z9 18 U1 0 U2 0 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD FEB 28 PY 1991 VL 349 IS 6312 BP 745 EP 746 DI 10.1038/349745a0 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EZ666 UT WOS:A1991EZ66600034 PM 2000145 ER EF