FN Thomson Reuters Web of Science™ VR 1.0 PT J AU NANOFF, C JACOBSON, KA STILES, GL AF NANOFF, C JACOBSON, KA STILES, GL TI THE A2 ADENOSINE RECEPTOR - GUANINE-NUCLEOTIDE MODULATION OF AGONIST BINDING IS ENHANCED BY PROTEOLYSIS SO MOLECULAR PHARMACOLOGY LA English DT Article ID BETA-ADRENERGIC RECEPTORS; ADENYLATE-CYCLASE; HORMONE RECEPTORS; BRAIN MEMBRANES; IDENTIFICATION; SUBUNIT; SOLUBILIZATION; PURIFICATION; MAGNESIUM; AFFINITY AB Agonist binding to the A2 adenosine receptor (A2AR) and its regulation by guanine nucleotides was studied using the newly developed radioligand I-125-2-[4-(2-{2-[(4-aminophenyl)methyl-carbonylamino]ethylaminnocarbonyl}ethyl)phenyl]ethylamino-5'-N-ethylcarboxamidoadenosine (I-125-PAPA-APEC) and its photo-affinity analog I-125-azido-PAPA-APEC. A single protein of M(r) 45,000, displaying the appropriate A2AR pharmacology, is labeled in membranes from bovine striatum, PC12 cells, and frog erythrocytes. In DDT1 MF2 cells the labeled protein has a slightly lower molecular weight. Incorporation of I-125-azido-PAPA-APEC into membranes from rabbit striatum, however, reveals two specifically labeled peptides (M(r) approximately 47,000 and 38,000), both of which display A2AR pharmacology. Inhibition of protease activity leads to a decrease in the amount of the M(r) 38,000 protein, with only the M(r) 47,000 protein remaining. This suggests that the M(r) 38,000 peptide is a proteolytic product of the M(r) 47,000 A2AR protein. In membranes containing the intact undigested A2AR protein, guanine nucleotides induce a small to insignificant decrease in agonist binding, which is atypical of stimulatory G(s)-coupled receptors. This minimal effect is observed in rabbit striatal membranes prepared in the presence of protease inhibitors, as well as in the other tissues studied. Binding to rabbit striatal membranes that possess the partially digested receptor protein, however, reveals a 50% reduction in maximal specific agonist binding upon addition of guanine nucleotides. Inhibition of proteolysis in rabbit striatum, on the other hand, results in a diminished ability of guanine nucleotides to regulate agonist binding. Thus, the enhanced effectiveness of guanine nucleotides in rabbit striatal membranes is associated with the generation of the M(r) 38,000 peptide fragment. Guanosine 5'-(beta, gamma-imido)triphosphate reduces photoaffinity labeling by 55% in the M(r) 38,000 protein, whereas the labeling is decreased by only 28% in the M(r) 47,000 receptor protein. Our data, therefore, suggest that, unless proteolysis occurs, the A2AR in all tissues studied is tightly associated with the G(s) protein and displays minimal guanine nucleotide modulation of agonist binding, which makes the A2AR an atypical stimulatory receptor. C1 DUKE UNIV,MED CTR,DEPT MED,BOX 3444,DURHAM,NC 27710. DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710. NIDDKD,CHEM LABS,BETHESDA,MD 20892. RI Jacobson, Kenneth/A-1530-2009; OI Jacobson, Kenneth/0000-0001-8104-1493; nanoff, christian/0000-0002-8857-2470 FU Intramural NIH HHS [Z01 DK031117-20, Z99 DK999999]; NHLBI NIH HHS [R01-HL-35134] NR 34 TC 70 Z9 70 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD FEB PY 1991 VL 39 IS 2 BP 130 EP 135 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EY502 UT WOS:A1991EY50200006 PM 1899902 ER PT J AU CHU, E DRAKE, JC KOELLER, DM ZINN, S JAMISDOW, CA YEH, GC ALLEGRA, CJ AF CHU, E DRAKE, JC KOELLER, DM ZINN, S JAMISDOW, CA YEH, GC ALLEGRA, CJ TI INDUCTION OF THYMIDYLATE SYNTHASE ASSOCIATED WITH MULTIDRUG RESISTANCE IN HUMAN BREAST AND COLON CANCER CELL-LINES SO MOLECULAR PHARMACOLOGY LA English DT Article ID CHINESE-HAMSTER CELLS; GENE AMPLIFICATION; MESSENGER-RNA; BIOCHEMICAL-CHARACTERIZATION; CARCINOMA-CELLS; FUNCTIONAL CDNA; HEPATOMA-CELLS; L1210 CELLS; 5-FLUOROURACIL; SYNTHETASE AB A series of Adriamycin-resistant human breast MCF-7 and human colon DLD-1 cancer cell lines were established by stepwise selection. The concentration of Adriamycin required to inhibit cell proliferation by 50% (IC50) in the parent breast line (MCF-7), Adriamycin-resistant lines (MCF-AD5 and MCF-Ad10), and a 5-fluorouracil (5-FU)-revertant line (MCF-R) was 0.005, 3.3, 6, and 4.9-mu-M, respectively. The Adriamycin IC50 value for the resistant colon line (DLD-Ad) was 8.2-mu-M, 68-fold higher than that for its parent line (DLD-1) (IC50 = 0.12-mu-M). The MCF-Ad5 and MCF-Ad10 cells were cross-resistant to 5-FU, with respective 5-FU IC50 values of 11.7 and 22.5-mu-M, or 7.3- and 14-fold less sensitive than their parent MCF-7 (IC50 = 1.6-mu-M) line. The MCF-R line completely reverted in sensitivity to 5-FU, with an IC50 of 1.7-mu-M. The resistant DLD-Ad line was 3.5-fold more resistant to 5-FU than was the parent DLD-1 line. Using both the 5-fluoro-2'-deoxyuridine-5'-monophosphate binding and catalytic assays for measurement of thymidylate synthase (TS) activity, there was significantly increased TS activity in the resistant MCF-Ad5 (2.4- and 2.5-fold), MCF-Ad10 (11.5- and 6.8-fold), and DLD-Ad (4.8- and 10.7-fold) lines, for binding and catalytic assays, respectively, compared with their parent MCF-7 and DLD-1 lines. The level of TS in cytosolic extracts, as determined by Western immunoblot analysis, was markedly increased for the resistant MCF-Ad5 (31-fold), MCF-Ad10 (46-fold), and DLD-Ad (52-fold) cells. Measurement of TS mRNA levels by Northern analysis revealed elevation of TS mRNA in the resistant MCF-AD5 (16.7-fold), MCF-Ad10 (31-fold), and DLD-Ad (55-fold) cells. Southern analysis showed that this increase in TS mRNA was not accompanied by any major rearrangements or amplification of the TS gene. Incorporation of 5-FU into the RNA and DNA of the resistant MCF-Ad10 cells was not significantly different, compared with that for parent MCF-7 cells. These studies suggest that exposure of human breast and human colon cancer cells to Adriamycin leads to overexpression of TS, with concomitant development of resistance to 5-FU. C1 NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892. RP CHU, E (reprint author), NCI,DIV CANC TREATMENT,MED BRANCH,BLDG 10,ROOM 12N226,BETHESDA,MD 20892, USA. NR 56 TC 84 Z9 86 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD FEB PY 1991 VL 39 IS 2 BP 136 EP 143 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EY502 UT WOS:A1991EY50200007 PM 1704999 ER PT J AU MONTPIED, P MORROW, AL KARANIAN, JW GINNS, EI MARTIN, BM PAUL, SM AF MONTPIED, P MORROW, AL KARANIAN, JW GINNS, EI MARTIN, BM PAUL, SM TI PROLONGED ETHANOL INHALATION DECREASES GAMMA-AMINOBUTYRIC ACIDA RECEPTOR ALPHA SUBUNIT MESSENGER-RNAS IN THE RAT CEREBRAL-CORTEX SO MOLECULAR PHARMACOLOGY LA English DT Article ID GABAA RECEPTOR; BENZODIAZEPINE BINDING; BRAIN SYNAPTONEUROSOMES; REGIONAL DISTRIBUTION; IONOPHORE COMPLEX; MICE; WITHDRAWAL; SITES; EXPRESSION; TOLERANCE AB Ethanol administration to rats by ethanol vapor inhalation (14 days) results in a 40-50% reduction in the level of gamma-aminobutyric acid(A) (GABA(A)) receptor alpha-1 subunit mRNAs [4.4 and 4.8 kilobases (kb)] in the cerebral cortex. The level of alpha-2 subunit mRNA (8.0 kb) was also reduced by 29%, whereas there was no effect of prolonged ethanol exposure on the level of alpha-3 subunit mRNA (3.1 kb). Ethanol exposure did not alter the steady state levels of cerebral cortical glutamic acid decarboxylase or beta-actin mRNAs. Moreover, no alterations in the levels of total RNA, poly(A)+ RNA, or rRNA were observed, suggesting that the ethanol-induced reductions in GABA(A) receptor alpha-1 and alpha-2 subunit mRNAs were not the result of a generalized effect of ethanol administration on transcription or mRNA turnover. These ethanol-induced reductions in GABA(A) receptor alpha subunit mRNAs may underlie alterations in GABA(A) receptor function or number observed following prolonged ethanol exposure in rats. C1 NIMH,CLIN NEUROSCI BRANCH,MOLEC NEUROGENET SECT,BETHESDA,MD 20892. NIAAA,CLIN STUDIES LAB,BETHESDA,MD 20892. RP MONTPIED, P (reprint author), NIMH,CLIN NEUROSCI BRANCH,MOLEC PHARMACOL SECT,BLDG 10,ROOM 4N214,BETHESDA,MD 20892, USA. NR 48 TC 177 Z9 177 U1 0 U2 4 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD FEB PY 1991 VL 39 IS 2 BP 157 EP 163 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EY502 UT WOS:A1991EY50200010 PM 1705000 ER PT J AU DUNIEC, ZM NETTESHEIM, P ELING, TE AF DUNIEC, ZM NETTESHEIM, P ELING, TE TI STIMULATION OF PROSTAGLANDIN-H SYNTHASE MESSENGER-RNA LEVELS AND PROSTAGLANDIN BIOSYNTHESIS BY PHORBOL ESTER - MEDIATION BY PROTEIN-KINASE-C SO MOLECULAR PHARMACOLOGY LA English DT Article ID ARACHIDONIC-ACID METABOLISM; VESICULAR GLAND MICROSOMES; CANINE KIDNEY-CELLS; GROWTH-FACTOR; ENDOPEROXIDE SYNTHETASE; DENOVO SYNTHESIS; MOUSE-BRAIN; MDCK CELLS; CYCLOOXYGENASE; PURIFICATION AB We have investigated the mechanisms by which the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulates prostaglandin E2 (PGE2) formation in the rat tracheal epithelial cell line EGV-6aigT, which can be grown in serum-free medium. The addition of TPA to cells that were prelabeled with [H-3]arachidonic acid did not enhance the release of [H-3]arachidonic acid and/or [H-3]PGE2, indicating that TPA does not stimulate phospholipase activity. The addition of exogenous arachidonic acid to cells pretreated with TPA resulted in increased PGE2 formation, compared with basal levels, indicating an elevation in prostaglandin H synthase (PHS) activity. PHS activity was maximal at 4 hr and was dependent upon the concentration of TPA. Actinomycin D and cycloheximide blocked the TPA response. The recovery of PHS activity of cells in which the existing PHS was inhibited by aspirin was enhanced by TPA treatment. TPA treatment enhanced the expression of PHS mRNA, as measured by Northern analysis. The addition of actinomycin D and cycloheximide reduced the TPA enhancement of PHS mRNA, indicating that the increase in PHS activity required de novo RNA and protein synthesis. Furthermore, pretreatment of the cells with protein kinase C inhibitors reduced the TPA-dependent stimulation of PHS activity and the expression of PHS mRNA. The data suggest that TPA-stimulated de novo synthesis of PHS is mediated by protein kinase C. C1 NIEHS,PULM PATHOBIOL LABS,POB 12233,RES TRIANGLE PK,NC 27709. NIEHS,MOLEC BIOPHYS LABS,RES TRIANGLE PK,NC 27709. NR 43 TC 14 Z9 14 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD FEB PY 1991 VL 39 IS 2 BP 164 EP 170 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EY502 UT WOS:A1991EY50200011 PM 1899904 ER PT J AU ROTHMAN, RB REID, A MAHBOUBI, A KIM, CH DECOSTA, BR JACOBSON, AE RICE, KC AF ROTHMAN, RB REID, A MAHBOUBI, A KIM, CH DECOSTA, BR JACOBSON, AE RICE, KC TI LABELING BY [H-3] 1,3-DI(2-TOLYL)GUANIDINE OF 2 HIGH-AFFINITY BINDING-SITES IN GUINEA-PIG BRAIN - EVIDENCE FOR ALLOSTERIC REGULATION BY CALCIUM-CHANNEL ANTAGONISTS AND PSEUDOALLOSTERIC MODULATION BY SIGMA LIGANDS SO MOLECULAR PHARMACOLOGY LA English DT Article ID ANTIPSYCHOTIC-DRUGS; OPIATE RECEPTORS; VAS-DEFERENS; RAT-BRAIN; DEXTROMETHORPHAN; PHENCYCLIDINE; RADIOLIGAND; NEURONS; SYSTEM AB Equilibrium binding studies with the sigma receptor ligand [H-3]1,3-di(2-tolyl)guanidine ([H-3]DTG) demonstrated two high affinity binding sites in membranes prepared from guinea pig brain. The apparent K(d) values of DTG for sites 1 and 2 were 11.9 and 37.6 nM, respectively. The corresponding B(max) values were 1045 and 1423 fmol/mg of protein. Site 1 had high affinity for (+)-pentazocine, haloperidol, (R)-(+)-PPP, carbepentane, and other sigma ligands, suggesting a similarity with the dextromethorphan/sigma-1 binding site described by Musacchio et al. [Life Sci. 45:1721-1732 (1989)]. Site 2 had high affinity for DTG and haloperiodl (K(i = 36.1 nM) and low affinity for most other sigma ligands. Kinetic experiments demonstrated that [H-3]DTG dissociated in a biphasic manner from both site 1 and site 2. DTG and haloperidol increased the dissociation rate of [H-3]DTG from site 1 and site 2, demonstrating the presence of pseudoallosteric interactions. Inorganic calcium channel blockers such as Cd2+ selectively increased the dissociation rate of [H-3]DTG from site 2, suggesting an association of this binding site with calcium channels. C1 NIADDKD,MED CHEM LAB,BETHESDA,MD 20892. RP ROTHMAN, RB (reprint author), NIMH,CLIN SCI LAB,RECEPTOR STUDIES UNIT,BLDG 10-3D41,BETHESDA,MD 20892, USA. NR 54 TC 127 Z9 128 U1 1 U2 3 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD FEB PY 1991 VL 39 IS 2 BP 222 EP 232 PG 11 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EY502 UT WOS:A1991EY50200019 PM 1847495 ER PT J AU OSGOOD, C ZIMMERING, S MASON, JM AF OSGOOD, C ZIMMERING, S MASON, JM TI ANEUPLOIDY IN DROSOPHILA .2. FURTHER VALIDATION OF THE FIX AND ZESTE GENETIC TEST SYSTEMS EMPLOYING FEMALE DROSOPHILA-MELANOGASTER SO MUTATION RESEARCH LA English DT Article DE ANEUPLOIDY IN DROSOPHILA; FIX TEST SYSTEM; ZESTE TEST SYSTEM ID SACCHAROMYCES-CEREVISIAE; ASSEMBLY INVITRO; TUBULIN; NONDISJUNCTION; YEAST; MUTAGENESIS; INDUCTION; SOLVENTS; INDUCERS; BINDING AB Two sensitive genetic systems for the detection of germline aneuploidy employing Drosophila melanogaster females were described in the first paper of this series (Zimmering et al., submitted to Mutation Research). Designated FIX and ZESTE, these systems permit the rapid and efficient detection of exceptional offspring derived from aneuploid female germ cells. The current report presents test results from a survey of 8 additional chemicals that have been analyzed in both systems. The tested chemicals include: acetonitrile, cadmium chloride, carbendazim, dimethylsulfoxide (DMSO), methylmercury(II) chloride, methoxyethyl acetate, propionitrile and water. Excluding the negative control, water, only the fungicide carbendazim failed to induce aneuploidy in either test system. Of the remaining 6 chemicals one, methylmercury(II) chloride, was positive in the FIX system but not in ZESTE, while MEA was positive in ZESTE and borderline in FIX. The results provide little evidence of germ-cell stage specificity of response to the tested chemicals. Comparison of the induced rates of aneuploidy indicates that these can exhibit departures from simple additivity to the spontaneous rates: induced rates in the ZESTE system are generally higher and more variable than those from FIX. Possible reasons for the difference in responsiveness between FIX and ZESTE flies are discussed as is the question of the classification of those chemicals which induce chromosome loss events but not chromosome gains. C1 BROWN UNIV,DIV BIOL & MED,PROVIDENCE,RI 02912. NIEHS,CELLULAR & GENET TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. RP OSGOOD, C (reprint author), OLD DOMINION UNIV,DEPT BIOL SCI,NORFOLK,VA 23529, USA. FU NIEHS NIH HHS [ES-55095, ES-55106] NR 34 TC 10 Z9 10 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD FEB PY 1991 VL 259 IS 2 BP 147 EP 163 DI 10.1016/0165-1218(91)90049-R PG 17 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA EX064 UT WOS:A1991EX06400004 PM 1899717 ER PT J AU OLLO, C JOHNSON, R GRAFMAN, J AF OLLO, C JOHNSON, R GRAFMAN, J TI SIGNS OF COGNITIVE CHANGE IN HIV DISEASE - AN EVENT-RELATED BRAIN POTENTIAL STUDY SO NEUROLOGY LA English DT Article ID AIDS DEMENTIA COMPLEX; ELECTROPHYSIOLOGICAL DIFFERENCES; HUNTINGTONS-DISEASE; PARKINSONS-DISEASE; P300; P3-COMPONENT; PERFORMANCE; DEPRESSION; INFECTION; DIAGNOSIS C1 NINCDS,MED NEUROL BRANCH,COGNITIV NEUROSCI SECT,BLDG 10,ROOM 5C422,BETHESDA,MD 20892. NIMH,CLIN SCI LAB,BETHESDA,MD 20892. OI Grafman, Jordan H./0000-0001-8645-4457 NR 42 TC 67 Z9 73 U1 1 U2 1 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0028-3878 J9 NEUROLOGY JI Neurology PD FEB PY 1991 VL 41 IS 2 BP 209 EP 215 PN 1 PG 7 WC Clinical Neurology SC Neurosciences & Neurology GA EX218 UT WOS:A1991EX21800010 PM 1992363 ER PT J AU YOUNG, WS HSU, AC AF YOUNG, WS HSU, AC TI OBSERVATIONS ON THE SIMULTANEOUS USE OF DIGOXIGENINLABELED AND RADIOLABELED OLIGODEOXYRIBONUCLEOTIDE PROBES FOR HYBRIDIZATION HISTOCHEMISTRY SO NEUROPEPTIDES LA English DT Article RP YOUNG, WS (reprint author), NIMH,CELL BIOL LAB,BLDG 36,ROOM 2D10,BETHESDA,MD 20892, USA. RI Young, W Scott/A-9333-2009 OI Young, W Scott/0000-0001-6614-5112 NR 2 TC 21 Z9 22 U1 0 U2 0 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH, MIDLOTHIAN, SCOTLAND EH1 3AF SN 0143-4179 J9 NEUROPEPTIDES JI Neuropeptides PD FEB PY 1991 VL 18 IS 2 BP 75 EP 75 DI 10.1016/0143-4179(91)90003-2 PG 1 WC Endocrinology & Metabolism; Neurosciences SC Endocrinology & Metabolism; Neurosciences & Neurology GA EW898 UT WOS:A1991EW89800003 PM 2017300 ER PT J AU COHEN, SR KIMES, AS LONDON, ED AF COHEN, SR KIMES, AS LONDON, ED TI MORPHINE DECREASES CEREBRAL GLUCOSE-UTILIZATION IN LIMBIC AND FOREBRAIN REGIONS WHILE PAIN HAS NO EFFECT SO NEUROPHARMACOLOGY LA English DT Article DE GLUCOSE UTILIZATION; 2-DEOXYGLUCOSE; MORPHINE; PAIN; OPIOID; ANALGESIA ID BRAIN-STEM STIMULATION; FORMALIN TEST; ELECTRICAL-STIMULATION; SELF-STIMULATION; HABENULAR NUCLEI; RAPHE MAGNUS; RAT; ANALGESIA; CONNECTIONS; RESPONSES AB Morphine alters affective states in humans and produces reinforcement in humans and animal subjects. Therefore, the present study was designed to determine whether morphine affected the functional activity in brain structures involved in emotion and motivation. Since opioid-induced analgesia largely reflects an influence on the emotional component of pain, the effect of pain on activity in these structures was also studied. Local rates of glucose utilization were measured by the 2-deoxy-D[1-C-14]glucose method in the brains of rats, that received morphine or saline, with or without a painful stimulus. Many rostral, and particularly diencephalic, nuclei showed decreased glucose utilization in morphine-treated rats, although caudal regions were mostly unaffected. There were dose-dependent decreases in glucose utilization with doses between 1 and 10 mg/kg morphine. However, nociceptive stimulation, with either formalin or tail-immersion, produced no significant effect on cerebral glucose utilization. The 2-deoxy-D-[1-C-14]-glucose technique, as used here, may lack adequate sensitivity to delineate areas of the brain mediating nociception in rats. C1 NATL INST DRUG ABUSE,ADDICT RES CTR,NEUROSCI BRANCH,NEUROPHARMACOL LAB,POB 5180,BALTIMORE,MD 21224. RI Cohen, S. Robin/D-2209-2014 OI Cohen, S. Robin/0000-0003-2153-8486 NR 46 TC 27 Z9 27 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0028-3908 J9 NEUROPHARMACOLOGY JI Neuropharmacology PD FEB PY 1991 VL 30 IS 2 BP 125 EP 134 PG 10 WC Neurosciences; Pharmacology & Pharmacy SC Neurosciences & Neurology; Pharmacology & Pharmacy GA EX596 UT WOS:A1991EX59600003 PM 2030820 ER PT J AU WEISSMAN, AD CASANOVA, MF KLEINMAN, JE DESOUZA, EB AF WEISSMAN, AD CASANOVA, MF KLEINMAN, JE DESOUZA, EB TI PCP AND SIGMA RECEPTORS IN BRAIN ARE NOT ALTERED AFTER REPEATED EXPOSURE TO PCP IN HUMANS SO NEUROPSYCHOPHARMACOLOGY LA English DT Article DE PHENCYCLIDINE; DRUG RECEPTORS; PSYCHOSIS; SUBSTANCE ABUSE; NEUROTOXICITY; DRUG METABOLISM ID CENTRAL NERVOUS-SYSTEM; DOPAMINE NEURONAL-ACTIVITY; HUMAN FRONTAL-CORTEX; METHYL-D-ASPARTATE; GUINEA-PIG BRAIN; BINDING-SITES; ANTIPSYCHOTIC-DRUGS; RAT-BRAIN; PHENCYCLIDINE PCP; HIGH-AFFINITY AB The psychotomimetic effects of phencyclidine (PCP) in humans can persist or reappear months after the drug has been eliminated, suggesting that PCP can induce long-term changes in the brain. The present study examined whether repeated exposure to PCP in a human drug-addicted population was accompanied by alterations in either PCP or sigma binding sites in their postmortem brains as compared to suicide controls. Saturation studies using [H-3]TCP and [H-3]haloperidol in the presence of spiperone to measure PCP and sigma sites, respectively, revealed no significant differences in the affinity or density of binding sites between these two clinical populations in a variety of brain areas examined. The results suggest that these brain binding sites remain unperturbed in humans despite multiple challenges with PCP. Delayed psychotic episodes following the use of PCP may be attributed to other neurochemical changes that are initiated by interactions of PCP with these two binding sites. C1 NIMH,CLIN BRAIN DISORDER BRANCH,BETHESDA,MD 20892. RP WEISSMAN, AD (reprint author), NIDR,ADDICT RES CTR,NEUROSCI BRANCH,POB 5180,BALTIMORE,MD 21224, USA. NR 43 TC 6 Z9 6 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0893-133X J9 NEUROPSYCHOPHARMACOL JI Neuropsychopharmacology PD FEB PY 1991 VL 4 IS 2 BP 95 EP 102 PG 8 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA EZ503 UT WOS:A1991EZ50300003 PM 1851014 ER PT J AU LEVIN, HS EISENBERG, HM GARY, HE MARMAROU, A FOULKES, MA JANE, JA MARSHALL, LF PORTMAN, SM AF LEVIN, HS EISENBERG, HM GARY, HE MARMAROU, A FOULKES, MA JANE, JA MARSHALL, LF PORTMAN, SM TI INTRACRANIAL HYPERTENSION IN RELATION TO MEMORY FUNCTIONING DURING THE 1ST YEAR AFTER SEVERE HEAD-INJURY SO NEUROSURGERY LA English DT Article DE HEAD INJURY; INTRACRANIAL PRESSURE; MEMORY ID INTRA-CRANICAL PRESSURE; COMA AB The relationship between intracranial hypertension and residual memory deficit after closed head injury was evaluated using the 6-month and 1-year neurobehavioral outcome data obtained by the Traumatic Coma Data Bank. Intracranial pressure was analyzed using the percentage of time that it exceeded 20 mm Hg and the maximum value recorded during the first 72 hours after injury. Memory measures included recall of word lists, prose recall, and visual memory for designs that were obtained 6 months (n = 149) and 1 year (n = 132) after injury. Intracranial hypertension occurred in more than half of the Traumatic Coma Data Bank cohort who met the criteria for the neurobehavioral follow-up study. Linear regression analysis disclosed an effect of elevated intracranial pressure on some, but not all, measures of memory at 6 months, whereas the results were negative for the 1-year follow-up examination. We conclude that the elevation of intracranial pressure exerts little if any effect on later memory functioning, and that any effect it does have diminishes over 1 year in survivors of severe head injury. C1 UNIV TEXAS,MED BRANCH,DEPT NEUROL,GALVESTON,TX 77550. CTR DIS CONTROL,ATLANTA,GA 30333. VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT NEUROSURG,RICHMOND,VA 23298. UNIV CALIF SAN DIEGO,DIV NEUROSURG,LA JOLLA,CA 92093. NIH,OFF BIOMETRY,BETHESDA,MD 20892. UNIV VIRGINIA,DEPT NEUROSURG,CHARLOTTESVILLE,VA 22903. RP LEVIN, HS (reprint author), UNIV TEXAS,MED BRANCH,DIV NEUROSURG D-73,GALVESTON,TX 77550, USA. FU NINDS NIH HHS [N01-NS-3-2339, N01-NS-3-2340, N01-NS-3-2341] NR 12 TC 36 Z9 37 U1 2 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0148-396X J9 NEUROSURGERY JI Neurosurgery PD FEB PY 1991 VL 28 IS 2 BP 196 EP 200 PG 5 WC Clinical Neurology; Surgery SC Neurosciences & Neurology; Surgery GA EU430 UT WOS:A1991EU43000004 PM 1997886 ER PT J AU EHRENREICH, H ANDERSON, RW OGINO, Y RIECKMANN, P COSTA, T WOOD, GP COLIGAN, JE KEHRL, JH FAUCI, AS AF EHRENREICH, H ANDERSON, RW OGINO, Y RIECKMANN, P COSTA, T WOOD, GP COLIGAN, JE KEHRL, JH FAUCI, AS TI SELECTIVE AUTOREGULATION OF ENDOTHELINS IN PRIMARY ASTROCYTE CULTURES - ENDOTHELIN RECEPTOR MEDIATED POTENTIATION OF ENDOTHELIN-1 SECRETION SO NEW BIOLOGIST LA English DT Article DE ENDOTHELIN; SARAFOTOXIN; PRIMARY ASTROCYTE CULTURES; AUTOREGULATION; ENDOTHELIN RECEPTORS; INOSITOLPHOSPHOLIPID TURNOVER; AP1; MOBILITY SHIFT ASSAY ID SMOOTH-MUSCLE CELLS; VASOCONSTRICTOR PEPTIDE; CHROMOSOMAL ASSIGNMENT; GLIAL-CELLS; C-FOS; EXPRESSION; BRAIN; GENE; SARAFOTOXIN; MITOGENESIS C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NIAID,BIOL RESOURCES BRANCH,BETHESDA,MD 20892. NICHHD,THEORET & PHYS BIOL LAB,BETHESDA,MD 20892. OI costa, tommaso/0000-0002-8729-3357; Kehrl, John/0000-0002-6526-159X NR 35 TC 42 Z9 42 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD FEB PY 1991 VL 3 IS 2 BP 135 EP 141 PG 7 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK717 UT WOS:A1991GK71700008 PM 1648383 ER PT J AU EVA, A GRAZIANI, G ZANNINI, M MERIN, LM KHILLAN, JS OVERBEEK, PA AF EVA, A GRAZIANI, G ZANNINI, M MERIN, LM KHILLAN, JS OVERBEEK, PA TI DOMINANT DYSPLASIA OF THE LENS IN TRANSGENIC MICE EXPRESSING THE DBL ONCOGENE SO NEW BIOLOGIST LA English DT Article DE CATARACT; LENS CELL DIFFERENTIATION; MICROPHTHALMIA; DBL PROTEIN EXPRESSION ID B-CELL LYMPHOMA; CHLORAMPHENICOL ACETYLTRANSFERASE; TARGETED EXPRESSION; GENETIC ABLATION; PROTO-ONCOGENE; VIMENTIN GENE; OVEREXPRESSION; SEQUENCES; DIFFERENTIATION; NITROCELLULOSE C1 NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892. BAYLOR COLL MED,CULLEN EYE INST,HOUSTON,TX 77030. JEFFERSON INST MOLEC MED,DEPT BIOCHEM & MOLEC BIOL,PHILADELPHIA,PA 19107. BAYLOR COLL MED,DEPT CELL BIOL,HOWARD HUGHES MED INST,HOUSTON,TX 77030. BAYLOR COLL MED,INST MOLEC GENET,HOUSTON,TX 77030. RI Graziani, Grazia/G-5747-2012; Zannini, Mariastella/I-1735-2012; Eva, Alessandra/J-8268-2016; OI Eva, Alessandra/0000-0003-2949-078X; GRAZIANI, GRAZIA/0000-0002-0221-768X NR 39 TC 13 Z9 13 U1 1 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD FEB PY 1991 VL 3 IS 2 BP 158 EP 168 PG 11 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK717 UT WOS:A1991GK71700011 PM 2065011 ER PT J AU RAO, PN PETERSON, DM ACOSTA, CK BAHR, ML KIM, HK AF RAO, PN PETERSON, DM ACOSTA, CK BAHR, ML KIM, HK TI SYNTHESIS OF CIS-4-AMINOCYCLOHEXYL-D-ALANINE AND TRANS-4-AMINOCYCLOHEXYL-D-ALANINE DERIVATIVES AND DETERMINATION OF THEIR STEREOCHEMISTRY SO ORGANIC PREPARATIONS AND PROCEDURES INTERNATIONAL LA English DT Article ID ANTAGONISTS; HORMONE; DESIGN C1 NICHHD,BETHESDA,MD 20205. RP RAO, PN (reprint author), SW FDN BIOMED RES,DEPT ORGAN CHEM,POB 28147,SAN ANTONIO,TX 78228, USA. NR 9 TC 6 Z9 7 U1 0 U2 1 PU ORGANIC PREP PROCEDURES INC PI NEWTON HIGHLANDS PA PO BOX 9, NEWTON HIGHLANDS, MA 02161 SN 0030-4948 J9 ORG PREP PROCED INT JI Org. Prep. Proced. Int. PD FEB PY 1991 VL 23 IS 1 BP 103 EP 110 PG 8 WC Chemistry, Organic SC Chemistry GA EW370 UT WOS:A1991EW37000004 ER PT J AU BURKE, TR AF BURKE, TR TI A DIRECT APPROACH TOWARD THE SYNTHESIS OF ANALOGS OF ERBSTATIN SO ORGANIC PREPARATIONS AND PROCEDURES INTERNATIONAL LA English DT Note ID PROTEIN-KINASE; INHIBITOR RP BURKE, TR (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MED CHEM LAB,BETHESDA,MD 20892, USA. RI Burke, Terrence/N-2601-2014 NR 11 TC 4 Z9 4 U1 0 U2 0 PU ORGANIC PREP PROCEDURES INC PI NEWTON HIGHLANDS PA PO BOX 9, NEWTON HIGHLANDS, MA 02161 SN 0030-4948 J9 ORG PREP PROCED INT JI Org. Prep. Proced. Int. PD FEB PY 1991 VL 23 IS 1 BP 127 EP 130 PG 4 WC Chemistry, Organic SC Chemistry GA EW370 UT WOS:A1991EW37000011 ER PT J AU HYLDEN, JLK NAHIN, RL TRAUB, RJ DUBNER, R AF HYLDEN, JLK NAHIN, RL TRAUB, RJ DUBNER, R TI EFFECTS OF SPINAL KAPPA-OPIOID RECEPTOR AGONISTS ON THE RESPONSIVENESS OF NOCICEPTIVE SUPERFICIAL DORSAL HORN NEURONS SO PAIN LA English DT Article DE SPINAL CORD; NOCICEPTION; DYNORPHIN; U-50,488H; LAMINA-I; (RAT) ID DYNORPHIN-A; RAT; CORD; PROJECTION; MECHANISMS; ANTINOCICEPTION; INFLAMMATION; RESPONSES; MORPHINE; INJURY AB Spinal cord application of the kappa-opioid receptor agonists dynorphin (50 nmol) or (1S,2S)U-50,488H (0.19-1.9-mu-mol) produced changes in the excitability of some superficial dorsal horn nociceptive neurons. One-third of the cells exhibited expansion of their receptive fields as defined using mechanical stimuli following a spinal kappa agonist (dynorphin or U-50,488H); receptive field expansions were of the same order as those observed immediately after a conditioning electrical stimulus applied to a peripheral nerve. In addition, spinal U-50,488H produced changes in mechanical and thermal thresholds of the majority of superficial dorsal horn neurons. These changes were dose-dependent. Facilitation of responses occurred at lower doses and inhibition occurred primarily at higher doses, but these effects were not reversed by subsequent administration of naloxone. The data are consistent with the hypothesis that one action of increases in spinal dynorphin levels due to peripheral inflammation, tissue injury or nerve damage, is to contribute to enhanced neuronal excitability in superficial dorsal horn neurons. RP HYLDEN, JLK (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 30,ROOM B-20,BETHESDA,MD 20892, USA. OI Traub, Richard/0000-0001-8633-6311 NR 22 TC 101 Z9 102 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3959 J9 PAIN JI Pain PD FEB PY 1991 VL 44 IS 2 BP 187 EP 193 DI 10.1016/0304-3959(91)90136-L PG 7 WC Anesthesiology; Clinical Neurology; Neurosciences SC Anesthesiology; Neurosciences & Neurology GA FA636 UT WOS:A1991FA63600014 PM 1675783 ER PT J AU ROILIDES, E BLACK, C REIMER, C RUBIN, M VENZON, D PIZZO, PA AF ROILIDES, E BLACK, C REIMER, C RUBIN, M VENZON, D PIZZO, PA TI SERUM IMMUNOGLOBULIN-G SUBCLASSES IN CHILDREN INFECTED WITH HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 SO PEDIATRIC INFECTIOUS DISEASE JOURNAL LA English DT Article DE IGG SUBCLASSES; HUMAN IMMUNODEFICIENCY VIRUS-1 INFECTION; CHILDREN ID ANTIBODY-RESPONSE; ACQUIRED IMMUNODEFICIENCY; LYMPHADENOPATHY SYNDROME; RECURRENT INFECTIONS; DEFICIENCY; AIDS; RATIOS; ASSAYS AB We studied serum concentrations of IgG subclasses in 47 human immunodeficiency virus 1-infected (17 asymptomatic and 30 symptomatic) children. Thirty-nine of 47 (83%) had an abnormality of at least 1 subclass. Sixteen had only elevated IgG1, 6 had only elevated IgG3 and 12 had elevated IgG1 and IgG3 concentrations. IgG2, IgG4 and combined IgG2-IgG4 deficiency was found in 3, 4 and 4 patients, respectively. IgG2 concentrations did not differ between patients with (n = 23) or without (n = 24) bacterial infections. Additionally the number of bacterial infections was similar between the patients with normal or low IgG2 and/or low IgG4. These data indicate that IgG subclass abnormalities are found in most children with human immunodeficiency virus 1 infection, but quantitative deficiencies of specific subclasses do not appear to explain the high frequency of bacterial infections occurring in these patients. C1 NCI,PEDIAT BRANCH,NCI BLDG,RM 13N240,BETHESDA,MD 20892. NCI,PEDIAT ONCOL BRANCH,INFECT DIS SECT,BETHESDA,MD 20892. NCI,BIOSTAT & DATA MANAGEMENT SECT,BETHESDA,MD 20892. CTR DIS CONTROL,CTR INFECT DIS,DIV IMMUNOL ONCOL & HEMATOL DIS,ATLANTA,GA 30333. RI Venzon, David/B-3078-2008 NR 33 TC 20 Z9 20 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0891-3668 J9 PEDIATR INFECT DIS J JI Pediatr. Infect. Dis. J. PD FEB PY 1991 VL 10 IS 2 BP 134 EP 139 DI 10.1097/00006454-199102000-00012 PG 6 WC Immunology; Infectious Diseases; Pediatrics SC Immunology; Infectious Diseases; Pediatrics GA EX489 UT WOS:A1991EX48900012 PM 2062605 ER PT J AU SPEAR, DJ MUNTANER, C GOLDBERG, SR KATZ, JL AF SPEAR, DJ MUNTANER, C GOLDBERG, SR KATZ, JL TI METHOHEXITAL AND COCAINE SELF-ADMINISTRATION UNDER FIXED-RATIO AND 2ND-ORDER SCHEDULES SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Article DE METHOHEXITAL; COCAINE; SQUIRREL MONKEY; SELF-ADMINISTRATION; FIXED-RATIO SCHEDULE; 2ND-ORDER SCHEDULE ID RHESUS-MONKEYS; DRUGS AB Behavior maintained by either cocaine or methohexital was compared under two different schedules of drug delivery. Under a fixed-ratio 10 schedule, each tenth response produced an injection and responding was characterized by pauses alternating with high rates that were sustained until the drug injection. Under a second-order schedule, each tenth response produced a brief visual stimulus, and the first sequence of ten responses emitted after the lapse of a ten-minute interval produced the stimulus and the drug injection. Responding under the second-order schedule was characterized by an overall positive acceleration in responding that consisted of fixed-ratio response patterns terminating in the presentation of a brief stimulus. Under either schedule, each drug maintained maximal rates of responding at intermediate doses. In most respects, rates and patterns of responding depended more on the schedule of drug delivery than on the particular drug maintaining responding. C1 NIDA,ADDICT RES CTR,POB 5180,BALTIMORE,MD 21224. RI Muntaner, C/A-5043-2010 NR 15 TC 19 Z9 19 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD FEB PY 1991 VL 38 IS 2 BP 411 EP 416 DI 10.1016/0091-3057(91)90300-Q PG 6 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA EY519 UT WOS:A1991EY51900030 PM 2057509 ER PT J AU ENGEL, BT TALAN, MI AF ENGEL, BT TALAN, MI TI AUTONOMIC BLOCKADE DOES NOT PREVENT LEARNED HEART-RATE ATTENUATION DURING EXERCISE SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE OPERANT CONDITIONING OF HEART RATE; CENTRAL COMMAND; EXERCISE; AUTONOMIC DRUGS ID NONHUMAN-PRIMATES; DYNAMIC EXERCISE AB Each of three monkeys was operantly conditioned to slow its heart, to exercise (lift weights) and to attenuate the tachycardia of exercise by combining these two skills. Each was further tested during beta-adrenergic blockade (atenolol), combined alpha-adrenergic blockade (prazosin) and beta-adrenergic blockade, or cholinergic blockade (methylatropine). During all experiments heart rate, stroke volume, intraarterial blood pressure, O2 consumption, and CO2 production were recorded on a beat-to-beat basis. Each animal was able to attenuate the tachycardia of exercise under each of the drug conditions, indicating that "central command" is not the expression of fixed, cardiovascular and pulmonary reflexes elicited by somato-motor commands, but rather is an adaptive behavior, determined by environmental contingencies and mediated by cardiovascular and pulmonary as well as somato-motor commands. The ability of the animals to perform with greater cardiac efficiency during the combined exercise and heart rate slowing task relative to the exercise-only task was not affected by sympathetic blockade; however, parasympathetic blockade did reduce cardiac efficiency. RP ENGEL, BT (reprint author), NIA,GERONTOL RES CTR,BEHAV SCI LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 15 TC 6 Z9 6 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD FEB PY 1991 VL 49 IS 2 BP 373 EP 382 DI 10.1016/0031-9384(91)90058-V PG 10 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA EZ611 UT WOS:A1991EZ61100024 PM 2062910 ER PT J AU SONG, W SI, SL XU, XJ PU, QL PANNELL, LK HIGHET, RJ AF SONG, W SI, SL XU, XJ PU, QL PANNELL, LK HIGHET, RJ TI STUDIES ON THE CHEMICAL-CONSTITUENTS OF PREMNA-FULVA SO PLANTA MEDICA LA English DT Letter ID NATURAL-PRODUCTS; LUPEOL; C-13; ACID C1 GUANGXI COLL TRADIT CHINESE MED,DEPT PHARM,NANNING,PEOPLES R CHINA. NIDDK,BIOORGAN CHEM LAB,BETHESDA,MD 20892. NHLBI,CHEM LAB,BETHESDA,MD 20892. NR 6 TC 0 Z9 0 U1 0 U2 3 PU GEORG THIEME VERLAG PI STUTTGART PA P O BOX 30 11 20, D-70451 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD FEB PY 1991 VL 57 IS 1 BP 93 EP 94 DI 10.1055/s-2006-960035 PG 2 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA EY917 UT WOS:A1991EY91700032 PM 17226132 ER PT J AU BEGLEY, CG VISVADER, J GREEN, AR APLAN, PD METCALF, D KIRSCH, IR GOUGH, NM AF BEGLEY, CG VISVADER, J GREEN, AR APLAN, PD METCALF, D KIRSCH, IR GOUGH, NM TI MOLECULAR-CLONING AND CHROMOSOMAL LOCALIZATION OF THE MURINE HOMOLOG OF THE HUMAN HELIX-LOOP-HELIX GENE SCL SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE HEMATOPOIESIS; ONCOGENE; TRANSCRIPTION FACTOR; GENETIC LINKAGE; RECOMBINANT-INBRED STRAINS ID DNA-BINDING MOTIF; T-CELL LEUKEMIA; IMMUNOGLOBULIN ENHANCERS; TRANSLOCATION; SEQUENCE; MYC; PROTEINS; REGION; MYOD; LINE AB The human SCL gene is a member of the family of genes that encode the helix-loop-helix (HLH) class of DNA-binding proteins. A murine SCL cDNA was isolated from a normal macrophage cDNA library by using HLH-specific oligonucleotides as hybridization probes. The coding region is 987 base pairs and encodes a predicted protein of 34 kDa. The nucleotide sequence of the coding region shows 88% identity to the human SCL gene, and the amino acid sequence is 94% identical. The HLH motif and upstream hydrophilic region are entirely conserved in the murine and human proteins. The identity between the mouse and human sequences was less marked in the 5' and 3' untranslated regions. Two murine SCL transcripts that differ in the 3' noncoding region have been detected in fetal liver and various cell lines. Variation was also observed in the 5' untranslated region. Interestingly, immediately downstream of the protein-termination codon, both the human SCL sequence and the murine homolog share an E-box element-the suggested target site for DNA binding of HLH proteins. The murine SCL homolog was mapped to the central part of chromosomes 4. C1 ROYAL MELBOURNE HOSP,DEPT DIAGNOST HAEMATOL,PARKVILLE,VIC 3050,AUSTRALIA. USN,MED BRANCH,BETHESDA,MD 20814. NCI,PEDIAT ONCOL BRANCH,BETHESDA,MD 20814. RP BEGLEY, CG (reprint author), ROYAL MELBOURNE HOSP,WALTER & ELIZA HALL INST MED RES,PARKVILLE,VIC 3050,AUSTRALIA. RI Aplan, Peter/K-9064-2016 NR 39 TC 87 Z9 89 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 3 BP 869 EP 873 DI 10.1073/pnas.88.3.869 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EW400 UT WOS:A1991EW40000039 PM 1704135 ER PT J AU POON, B DIXON, D ELLIS, L ROTH, RA RUTTER, WJ WANG, LH AF POON, B DIXON, D ELLIS, L ROTH, RA RUTTER, WJ WANG, LH TI MOLECULAR-BASIS OF THE ACTIVATION OF THE TUMORIGENIC POTENTIAL OF GAG INSULIN-RECEPTOR CHIMERAS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID SARCOMA VIRUS UR2; NUCLEOTIDE-SEQUENCE; KINASE; PROTEIN; GENE; ONCOGENES; ANTIBODY; FAMILY; DOMAIN; CELLS AB A previous study showed that the human insulin receptor (IR) could be activated by insertion of a 3' portion of the cDNA encoding the beta-subunit into a retrovirus genome to form a Gag-IR fusion protein. While capable of transforming cells in culture, this IR cDNA-containing virus, called UIR, was not able to induce tumors in animals. Subsequently, we isolated a spontaneous sarcomagenic variant called UIR19t from the parental UIR. UIR19t was molecularly cloned, sequenced, and found to harbor two mutations. A 44-amino acid deletion immediately upstream from the transmembrane domain of the Gag-IR fusion protein removes all the extracellular sequence of the IR remaining in the original UIR construct. In addition, a single nucleotide deletion at the 3' end results in truncation and replacement of the carboxyl-terminal 12 amino acids by 4 new amino acids. The specific kinase activity of UIR19t is 4- to 5-fold higher than that of the parental UIR. However, no new cellular substrates were detected in UIR19t-transformed cells as compared to UIR cells. Viruses containing either the 5' or the 3' deletion mutation were constructed and assessed for their biological function. Our data indicate that the 5' deletion alone is sufficient to confer tumorigenic ability. We conclude that sequence immediately upstream from the transmembrane domain imposes a negative effect on the transforming and tumorigenic potential of the Gag-IR fusion protein. C1 CUNY MT SINAI SCH MED,DEPT MICROBIOL,ONE GUSTAVE LEVY PL,NEW YORK,NY 10029. NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709. HOWARD HUGHES MED INST,RES LABS,DALLAS,TX. UNIV TEXAS,DALLAS,TX 75235. STANFORD UNIV,DEPT PHARMACOL,STANFORD,CA 94305. UNIV CALIF SAN FRANCISCO,HORMONE RES INST,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143. FU NCI NIH HHS [CA29339] NR 22 TC 20 Z9 20 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 3 BP 877 EP 881 DI 10.1073/pnas.88.3.877 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EW400 UT WOS:A1991EW40000041 PM 1846965 ER PT J AU HUBBELL, HR BOYER, JE ROANE, P BURCH, RM AF HUBBELL, HR BOYER, JE ROANE, P BURCH, RM TI CYCLIC-AMP MEDIATES THE DIRECT ANTIPROLIFERATIVE ACTION OF MISMATCHED DOUBLE-STRANDED-RNA SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE ANTIPROLIFERATIVE EFFECTS; INTERFERON INDEPENDENCE; ADENYLATE CYCLASE ID DEPENDENT PROTEIN-KINASE; HUMAN INTERFERON; DNA-SYNTHESIS; TUMOR-CELLS; 3T3 CELLS; GROWTH; TRANSCRIPTION; INHIBITION; INDUCTION; ELEMENT AB Previous experiments have demonstrated that double-stranded RNAs (dsRNAs) can exert an antiproliferative effect on human tumor cells, independent of interferon (IFN) induction. However, the mechanism by which dsRNAs inhibit tumor growth has not been elucidated. As a first step in determining the molecular events responsible for growth arrest, we have explored the role of signal transduction through the cAMP system in the antiproliferative effect of the mismatched dsRNA, r(I)n.r(C12,U)n (Ampligen). These studies utilized the human glioma cell line A1235, which does not produce detectable levels of IFN-alpha, -beta, or -gamma in response to mismatched dsRNA treatment. Treatment of A1235 cells with mismatched dsRNA in combination with either 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), which inhibits cAMP-dependent protein kinase and protein kinase C, or N-(2-guanidinoethyl)-5-isoquinolinesulfonamide (HA1004), which preferentially inhibits the cAMP-dependent protein kinase, yielded an antagonism of the mismatched dsRNA-induced antiproliferative effect. Measurement of adenylate cyclase activation showed a dose-dependent increase in activity at antiproliferative mismatched dsRNA concentrations, but not at lower, nonantiproliferative doses. This increase in activity was rapid, seen as early as 30 sec after initiation of treatment, and it was sustained at peak levels for 1-2 hr. Analysis of the intracellular cAMP concentration gave similar kinetics of induction. Exposure of cells to the stable cAMP analogue dibutyryl cAMP yielded dose-dependent inhibition of cell growth. The cAMP phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine also inhibited proliferation. In contrast, neither H-7 nor HA1004 had an effect on growth inhibition induced by human natural IFN-alpha treatment. In addition, antiproliferative doses of IFN-alpha did not increase cAMP concentrations. These results indicate that the cAMP system is utilized by mismatched dsRNA as an early signal transduction mechanism for growth control. Furthermore, the antiproliferative effects induced by mismatched dsRNA and IFN can occur by different mechanisms of action. C1 HOWARD UNIV,COLL MED,DEPT MICROBIOL,WASHINGTON,DC 20059. NIMH,CELL BIOL LAB,BETHESDA,MD 20892. RP HUBBELL, HR (reprint author), HAHNEMANN UNIV,DEPT NEOPLAST DIS,BROAD & VINE ST,PHILADELPHIA,PA 19102, USA. FU NCI NIH HHS [1 PO1 CA29545] NR 49 TC 20 Z9 20 U1 1 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 3 BP 906 EP 910 DI 10.1073/pnas.88.3.906 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EW400 UT WOS:A1991EW40000047 PM 1846967 ER PT J AU CURCIO, MJ GARFINKEL, DJ AF CURCIO, MJ GARFINKEL, DJ TI SINGLE-STEP SELECTION FOR TY1 ELEMENT RETROTRANSPOSITION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE SACCHAROMYCES-CEREVISIAE; REVERSE TRANSCRIPTION; ARTIFICIAL INTRON ID SACCHAROMYCES-CEREVISIAE; GENE CONVERSION; SPT3 GENE; YEAST; TRANSPOSITION; MUTATIONS; SEQUENCE; MOVEMENT AB The yeast retrotransposon Ty1 has been tagged with a reporter gene that allows selection of RNA-mediated transposition events and is applicable to the study of retroelements in other organisms. The reporter gene is a yeast HIS3 gene interrupted by an artificial intron (AI) in the antisense orientation. The HIS3AI sequences were inserted into a Ty1 element such that the intron is on the sense strand of the Ty1 element; therefore, splicing and retrotransposition of marked Ty1 transcripts can give rise to His+ cells. Fusion of the Ty1-H3mHIS3AI element to the inducible GAL1 promoter resulted in a high frequency of histidine prototrophs upon galactose induction. Moreover, spontaneous His+ revertants derived from strains containing genomic TymHIS3AI elements are a result of retrotransposition. By using this assay, we estimated the Ty1 transposition rate to be between 3 X 10(-7) and 1 X 10(-5) transposition per Ty1 element per generation. Variations in the transposition rate of individual Ty1 elements are correlated with the relative abundance of their transcripts. C1 NATL CANC INST,FREDERICK CANC RES & DEV CTR,BASIC RES PROGRAM,ADV BIOSCI LABS,POB B,FREDERICK,MD 21702. OI Curcio, M. Joan/0000-0001-5361-3909 FU NCI NIH HHS [N01-CO-74101] NR 21 TC 183 Z9 185 U1 0 U2 3 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 3 BP 936 EP 940 DI 10.1073/pnas.88.3.936 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EW400 UT WOS:A1991EW40000053 PM 1846969 ER PT J AU KALEBIC, T KINTER, A POLI, G ANDERSON, ME MEISTER, A FAUCI, AS AF KALEBIC, T KINTER, A POLI, G ANDERSON, ME MEISTER, A FAUCI, AS TI SUPPRESSION OF HUMAN-IMMUNODEFICIENCY-VIRUS EXPRESSION IN CHRONICALLY INFECTED MONOCYTIC CELLS BY GLUTATHIONE, GLUTATHIONE ESTER, AND N-ACETYLCYSTEINE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE TUMOR NECROSIS FACTOR; INTERLEUKIN-6; PHORBOL 12-MYRISTATE 13-ACETATE ID TUMOR NECROSIS FACTOR; FACTOR-ALPHA; KAPPA-B; HIV-1-INFECTED PATIENTS; GENE-EXPRESSION; D-PENICILLAMINE; T-CELLS; HIV; ACTIVATION; TYPE-1 AB The effects of glutathione (GSH), glutathione ester (GSE), and N-acetyl-L-cysteine (NAC) on the induction of human immunodeficiency virus (HIV) expression were investigated in the chronically infected monocytic U1 cell line, a previously described cellular model for HIV latency. U1 cells constitutively express low levels of virus, which can be increased by phorbol 12-myristate 13-acetate (PMA), tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6), and other inducers. GSH, GSE, and NAC suppressed in a dose-dependent fashion the induction of HIV expression mediated by PMA, TNF-alpha, and IL-6, in the absence of cytotoxic or cytostatic effects. Reverse transcriptase activity, inducible by PMA, TNF-alpha, or IL-6, was decreased by 80-90% after pretreatment with GSH, GSE, or NAC. The induction of total HIV protein synthesis was also decreased appreciably after pretreatment with GSH, GSE, or NAC. The accumulation of HIV mRNA was substantially suppressed after pretreatment with NAC but to a lesser extent after pretreatment with GSH or GSE. Although PMA induces the expression of TNF-alpha in U1 cells, the suppressive effect of GSH, GSE, and NAC on PMA-induced HIV expression in U1 cells was not associated with the inhibition of TNF-alpha expression. The present findings, which elucidate relationships between cellular GSH and HIV expression, suggest that therapy with thiols may be of value in the treatment of HIV infection. C1 CORNELL UNIV,MED CTR,COLL MED,DEPT BIOCHEM,NEW YORK,NY 10021. RP KALEBIC, T (reprint author), NIAID,IMMUNOREGULAT LAB,BLDG 10,ROOM 11B-13,BETHESDA,MD 20892, USA. FU NIDDK NIH HHS [2 R37 DK-12034] NR 48 TC 295 Z9 297 U1 0 U2 3 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 3 BP 986 EP 990 DI 10.1073/pnas.88.3.986 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EW400 UT WOS:A1991EW40000063 PM 1704137 ER PT J AU BAILLON, JG NASHED, NT SAYER, JM JERINA, DM AF BAILLON, JG NASHED, NT SAYER, JM JERINA, DM TI CONTINUOUS MICROSPECTROPHOTOMETRIC MEASUREMENT OF DNA-POLYMERASE-ACTIVITY - APPLICATION TO THE KLENOW FRAGMENT OF ESCHERICHIA-COLI DNA-POLYMERASE-I AND HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE-TRANSCRIPTASE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE CIRCULAR DICHROISM; UV SPECTROPHOTOMETRY; ENZYME KINETICS ID PROCESSIVITY; INHIBITION; MECHANISM AB Progress of DNA- and/or RNA-directed DNA polymerization reactions can be measured continuously using circular dichroism (CD) or ultraviolet (UV) spectroscopy. In the presence of the Klenow fragment of Escherichia coli DNA polymerase I, a CD change of -0.27 +/- 0.06 millidegree at 248 nm and a UV change of -2.7 +/- 0.3 milliabsorbance units at 275 nm occur upon incorporation of 120 pmol of dTMP in a reaction volume of 120-mu-l (1-mu-M dTMP incorporation) into a synthetic template-primer, p(dA)40-60.p(dT)20. The transcription of poly(A).p(dT)12-18 by reverse transcriptases can also be monitored using these methods. Kinetic parameters for the polymerization reaction catalyzed by the Klenow fragment were determined from initial velocity measurements using CD or UV assays and were in close agreement with those measured by the standard single point radiochemical filtration assay. The generality of optical techniques for the measurement of DNA polymerase activity was shown by the use of a partially self-complementary hairpin-shaped oligonucleotide substrate for the Klenow fragment. Addition of a single nucleotide residue under steady-state conditions to this 35-mer at a concentration of 1.5-3-mu-M gave an easily measurable absorbance decrease at 275 nm, and the absorbance changes upon sequential addition of nucleotide units were additive. RP BAILLON, JG (reprint author), NIDDKD,BIOORGAN CHEM LAB,BETHESDA,MD 20892, USA. NR 22 TC 7 Z9 7 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 3 BP 1014 EP 1018 DI 10.1073/pnas.88.3.1014 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EW400 UT WOS:A1991EW40000069 PM 1704125 ER PT J AU REYNOLDS, SH ANNA, CK BROWN, KC WIEST, JS BEATTIE, EJ PERO, RW IGLEHART, JD ANDERSON, MW AF REYNOLDS, SH ANNA, CK BROWN, KC WIEST, JS BEATTIE, EJ PERO, RW IGLEHART, JD ANDERSON, MW TI ACTIVATED PROTOONCOGENES IN HUMAN LUNG-TUMORS FROM SMOKERS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE CARCINOGENESIS; POINT MUTATION; POLYMORPHISM; METASTASIS ID K-RAS ONCOGENE; METASTATIC PHENOTYPE; TRANSFORMING GENES; PROTO-ONCOGENE; B6C3F1 MOUSE; CANCER; ASSAY; CARCINOGENESIS; ADENOCARCINOMA; IDENTIFICATION AB Fourteen primary human lung tumor DNAs from smokers were analyzed for transforming activity by two DNA transfection assays. Activated protooncogenes were detected in 3 of 11 tumor DNAs by the NIH 3T3 focus assay, whereas activated protooncogenes were detected in 11 of 13 tumor DNAs by the NIH 3T3 cotransfection-nude mouse tumorigenicity assay. K- or NRAS genes activated by point mutation at codons 12 or 61 were detected in a large cell carcinoma, a squamous cell carcinoma, and 5 adenocarcinomas. An HRAS oncogene activated by a different mechanism was detected in an epidermoid carcinoma. One adenocarcinoma was found to contain an activated RAF gene. Two unidentified transforming genes were detected in a squamous cell carcinoma DNA and two adenocarcinoma DNAs. Eight of 10 lung adenocarcinomas that had formed metastases at the time of surgery were found to contain RAS oncogenes. No significant increase in metastasis was observed in the lung adenocarcinomas that contained one or more 6-kilobase EcoRI alleles of the LMYC gene. Overall, 12 of 14 (86%) of the lung tumor DNAs from smokers were found to contain activated protooncogenes. RAS oncogenes appear to play a role in the development of metastases in lung adenocarcinomas. C1 BETH ISRAEL MED CTR,NEW YORK,NY 10003. NYU MED CTR,NEW YORK,NY 10016. DUKE UNIV,MED CTR,DURHAM,NC 27710. RP REYNOLDS, SH (reprint author), NIEHS,RES TRIANGLE PK,NC 27709, USA. NR 40 TC 77 Z9 79 U1 1 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1085 EP 1089 DI 10.1073/pnas.88.4.1085 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700001 PM 1996309 ER PT J AU TURNER, B RAPP, U APP, H GREENE, M DOBASHI, K REED, J AF TURNER, B RAPP, U APP, H GREENE, M DOBASHI, K REED, J TI INTERLEUKIN-2 INDUCES TYROSINE PHOSPHORYLATION AND ACTIVATION OF P72-74 RAF-1 KINASE IN A T-CELL LINE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE LYMPHOKINE; SERINE; THREONINE KINASE; PROTOONCOGENE ID RECEPTOR-BETA-CHAIN; IL-2 RECEPTOR; MONOCLONAL-ANTIBODIES; SIGNAL TRANSDUCTION; MOLECULAR-CLONING; PROTEIN-KINASE; GROWTH-FACTORS; EXPRESSION; CDNA; LYMPHOCYTES AB Interleukin 2 (IL-2) is a lymphokine, produced by T cells upon antigenic or mitogenic stimulation, that is a critical regulator of T-cell proliferation. Although the binding of IL-2 to its receptor has been well characterized, the molecular mechanisms by which IL-2 transmits its signal from the membrane to the interior of the cell are poorly understood. Like most other growth factors, IL-2 causes rapid phosphorylation of proteins within its target cells. Unlike many other growth factors, however, the known subunits of the IL-2 receptor lack tyrosine-specific kinase activity, and little is known about the kinases whose activities are regulated by IL-2. Here we show that IL-2 (but not IL-4) induces rapid phosphorylation of the p72-74 serine/threonine-specific kinase encoded by the c-Raf-1 protooncogene in an IL-2-dependent murine T-cell line, CTLL-2, and that this phosphorylation is associated with increased kinase activity in p72-74 Raf-1-containing immune complexes. The concentration dependence of IL-2-mediated elevations in Raf-1 kinase activity correlated well with IL-2-stimulated proliferation of CTLL-2 cells. Furthermore, much of the IL-2-stimulated phorphorylation of p72-74 Raf-1 occurred on tyrosines. To our knowledge, the Raf-1 kinase represents the first endogenous substrate of an IL-2-regulated tyrosine kinase to be identified. C1 UNIV PENN,SCH MED,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104. NCI,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21701. FU NCI NIH HHS [CA47956] NR 36 TC 197 Z9 197 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1227 EP 1231 DI 10.1073/pnas.88.4.1227 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700030 PM 1996324 ER PT J AU YOUNG, ST PORRINO, LJ IADAROLA, MJ AF YOUNG, ST PORRINO, LJ IADAROLA, MJ TI COCAINE INDUCES STRIATAL C-FOS-IMMUNOREACTIVE PROTEINS VIA DOPAMINERGIC-D1 RECEPTORS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE FOS-RELATED ANTIGENS; PROTOONCOGENES; TRANSCRIPTION FACTOR AP-1; CAUDATE-PUTAMEN; NUCLEUS ACCUMBENS ID TRANSCRIPTIONAL REGULATION; ADENYLATE-CYCLASE; MESSENGER-RNA; CYCLIC-AMP; DYNORPHIN; SYSTEM; CELLS; GENE; DNA; JUN AB The protooncogene c-fos produces a phosphoprotein, Fos, which regulates gene transcription processes. In neuronal systems, Fos has been proposed to couple synaptic transmission to changes in gene expression by acting in the cell nucleus in concert with other proteins to form complexes in the promoter regions of target genes. We report here that the acute administration of a single dose of the indirect-acting dopaminergic agonist cocaine increases multiple Fos proteins in rat caudate nucleus. The increase is dose-dependent and is apparent immunocytochemically at 1 hr, maximal at 2 hr, and absent 48 hr after treatment. The increase seen immunocytochemically is composed of several molecular weight species as assessed by Western blotting of proteins from isolated striatal cell nuclei. Administration of the specific dopaminergic receptor antagonists sulpiride and SCH-23390 prior to cocaine support a significant role for D1 but not for D2 receptors in mediating this effect. These data indicate that D1 dopamine receptors are linked to a cellular immediate-early gene system(s) and suggest an action of cocaine at one or more levels of gene expression via modulation of transcriptional processes in activated cells. C1 NIMH,CEREBRAL METAB LAB,BETHESDA,MD 20892. NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 10,ROOM 1A09,BETHESDA,MD 20892. NINCDS,HOWARD HUGHES MED INST,SURG NEUROL BRANCH,BETHESDA,MD 20892. NR 52 TC 470 Z9 472 U1 1 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1291 EP 1295 DI 10.1073/pnas.88.4.1291 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700043 PM 1825356 ER PT J AU BRUNSWICK, M SAMELSON, LE MOND, JJ AF BRUNSWICK, M SAMELSON, LE MOND, JJ TI SURFACE-IMMUNOGLOBULIN CROSS-LINKING ACTIVATES A TYROSINE KINASE PATHWAY IN B-CELLS THAT IS INDEPENDENT OF PROTEIN-KINASE-C SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE LYMPHOCYTES-B; SIGNAL TRANSDUCTION ID MURINE LYMPHOCYTES-B; MONOCLONAL-ANTIBODIES; ANTI-IMMUNOGLOBULIN; CROSS-LINKING; IGM; PROLIFERATION; CALCIUM; PHOSPHOTYROSINE; MOBILIZATION; STIMULATION AB It has been found that the principal biochemical pathway activated in B cells stimulated by antigen- or anti-immunoglobulin-mediated crosslinking of surface immunoglobulin is that resulting in hydrolysis of phosphatidylinositol bisphosphate with generation of diacylglycerol and inositol trisphosphate. Recent evidence suggests that surface immunoglobulin-mediated B-cell activation can proceed without detectable increases in the concentration of either diacylglycerol or intracellular Ca2+ concentration, implicating involvement of other non-protein-kinase-C/Ca2+-dependent signal-transduction pathways. Therefore, we sought evidence for activation of a signaling pathway that is associated with growth regulation in other cell types - i.e., the protein-tyrosine kinases. We now show that crosslinking of membrane immunoglobulin by mitogenic antibodies leads to rapid tyrosine phosphorylation of several cellular substrates, consistent with the induction of a tyrosine kinase activity. This increase in tyrosine phosphorylation is weakly (if at all) stimulated by other B-cell mitogens, including phorbol esters and ionophores, and does not require the presence of detectable protein kinase C. Furthermore, inhibition of anti-immunoglobulin-stimulated phosphatidylinositol bisphosphate hydrolysis does not inhibit activation of this tyrosine kinase-dependent pathway. These findings suggest that occupancy of the membrane immunoglobulin receptor may induce multiple pathways of activation. C1 NICHHD,CELL BIOL & METAB BRANCH,BETHESDA,MD 20892. RP BRUNSWICK, M (reprint author), UNIFORMED SERV UNIV HLTH SCI,DEPT MED,BETHESDA,MD 20814, USA. FU NIAID NIH HHS [R01 AI24273, R01 AI27465] NR 42 TC 92 Z9 92 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1311 EP 1314 DI 10.1073/pnas.88.4.1311 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700047 PM 1705033 ER PT J AU BUSHMAN, FD CRAIGIE, R AF BUSHMAN, FD CRAIGIE, R TI ACTIVITIES OF HUMAN-IMMUNODEFICIENCY-VIRUS (HIV) INTEGRATION PROTEIN INVITRO - SPECIFIC CLEAVAGE AND INTEGRATION OF HIV DNA SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE RETROVIRAL DNA INTEGRATION; INTEGRASE PROTEIN; LONG TERMINAL REPEAT; INTEGRATIVE RECOMBINATION ID MURINE LEUKEMIA-VIRUS; RETROVIRAL INTEGRATION; NUCLEOTIDE-SEQUENCE; AIDS VIRUS; POL GENE; RECOMBINATION; TRANSPOSITION; PRODUCT; SITE AB Growth of human immunodeficiency virus (HIV) after infection requires the integration of a DNA copy of the viral RNA genome into a chromosome of the host. Here we present a simple in vitro system that carries out the integration reaction and the use of this system to probe the mechanism of integration. The only HIV protein necessary is the integration (IN) protein, which has been overexpressed in insect cells and then partially purified. DNA substrates are supplied as oligonucleotides that match the termini of the linear DNA product of reverse transcription. In the presence of HIV IN protein, oligonucleotide substrates are cleaved to generate the recessed 3' ends that are the precursor for integration, and the cleaved molecules are efficiently inserted into a DNA target. Analysis of reaction products reveals that HIV IN protein joins 3' ends of the viral DNA to 5' ends of cuts made by IN protein in the DNA target. We have also used this assay to characterize the sequences at the ends of the viral DNA involved in integration. The assay provides a simple screen for testing candidate inhibitors of HIV IN protein; some such inhibitors might have useful antiviral activity. RP BUSHMAN, FD (reprint author), NIDDKD,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 33 TC 365 Z9 371 U1 0 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1339 EP 1343 DI 10.1073/pnas.88.4.1339 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700053 PM 1847518 ER PT J AU EFTIMIE, R BRENNER, HR BUONANNO, A AF EFTIMIE, R BRENNER, HR BUONANNO, A TI MYOGENIN AND MYOD JOIN A FAMILY OF SKELETAL-MUSCLE GENES REGULATED BY ELECTRICAL-ACTIVITY SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE DENERVATION; TRANSCRIPTION; DIFFERENTIATION; INNERVATION ID RECEPTOR ALPHA-SUBUNIT; MESSENGER-RNA LEVELS; TRANSGENIC MICE; SOLEUS MUSCLE; MYC HOMOLOGY; EXPRESSION; SEQUENCE; ENHANCER; FIBROBLASTS; PROTEINS AB Myogenin and MyoD are proteins that bind to the regulatory regions of a battery of skeletal muscle genes and can activate their transcription during muscle differentiation. We have recently found that both proteins interact with the enhancer of the nicotinic acetylcholine receptor (nAChR) alpha-subunit, a gene that is regulated by innervation. This observation prompted us to study if myogenin and MyoD transcript levels are also regulated by skeletal muscle innervation. Using Northern blot analysis, we found that MyoD and myogenin mRNA levels begin to decline at embryonic day 17 and attain adult levels in muscle of newborn and 3-week-old mice, respectively. In contrast, nAChR mRNAs are highest in newborn and 1-week-old mouse muscle and decline thereafter to reach adult levels in 3-week-old mice. To determine if the down-regulation of myogenin and MyoD mRNA levels during development is due to innervation, we quantitated message levels in adult calf muscles after denervation. We found that in denervated muscle myogenin and MyoD mRNAs reach levels that are approximately 40- and 15-fold higher than those found in innervated muscle. Myogenin mRNAs begin to accumulate rapidly between 8 and 16 hr after denervation, and MyoD transcripts levels begin to increase sharply between 16 hr and 1 day after denervation. The increases in myogenin and MyoD mRNA levels precede the rapid accumulation of nAChR alpha-subunit transcripts; receptor mRNAs begin to accumulate significantly after 1 day of denervation. The effects of denervation are specific because skeletal alpha-actin mRNA levels are not affected by denervation. In addition, we found that the repression of myogenin and MyoD expression by innervation is due, at least in part, to "electrical activity." Direct stimulation of soleus muscle with extracellular electrodes repressed the increase of myogenin and MyoD transcripts after denervation by 4- to 3-fold, respectively. In view of these results, it is interesting to speculate that myogenin and/or MyoD may regulate a repertoire of skeletal muscle genes that are down-regulated by electrical activity. C1 NICHHD,DEV NEUROBIOL LAB,MOLEC NEUROBIOL UNIT,BLDG 36,ROOM 2A-21,BETHESDA,MD 20892. UNIV BASEL,INST PSYCHOL,CH-4051 BASEL,SWITZERLAND. NR 46 TC 275 Z9 279 U1 1 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1349 EP 1353 DI 10.1073/pnas.88.4.1349 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700055 PM 1705035 ER PT J AU HARRIS, JW POHL, LR MARTIN, JL ANDERS, MW AF HARRIS, JW POHL, LR MARTIN, JL ANDERS, MW TI TISSUE ACYLATION BY THE CHLOROFLUOROCARBON SUBSTITUTE 2,2-DICHLORO-1,1,1-TRIFLUOROETHANE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE HEPATIC METABOLISM; HYDROCHLOROFLUOROCARBONS; TRIFLUOROACETYLATED PROTEINS; NEOANTIGENS; F-19 NMR ID HALOTHANE-ASSOCIATED HEPATITIS; ULTRAVIOLET-RADIATION; COVALENT BINDING; METABOLIC BASIS; ANTIBODIES; SERA; HEPATOTOXICITY; HEPATOCYTES; NEOANTIGENS AB Hydrochlorofluorocarbons (HCFCs) are being developed as substitutes for ozone-depleting chlorofluorocarbons (CFCs); because widespread human exposure to HCFCs may be expected, it is important to evaluate their toxicities thoroughly. Here we report studies on the bioactivation of the CFC substitute 2,2-dichloro-1,1,1-trifluorethane (HCFC-123) to an electrophilic intermediate that reacts covalently with liver proteins. HCFC-123 and its analog halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) were studied in rats by F-19 NMR spectroscopy, and we found that a trifluoroacetylated lysine adduct was formed with liver proteins. Also, the pattern of proteins immunoreactive with hapten-specific anti-trifluoroacetylprotein antibodies was identical in livers of HCFC-123- and halothane-exposed rats. Because halothane causes an idiosyncratic, and sometimes fatal, hepatitis that is associated with an immune response against several trifluoroacetylated liver proteins, the present findings raise the possibility that humans exposed to HCFC-123 or structurally related HCFCs may be at risk of developing an immunologically mediated hepatitis. C1 UNIV ROCHESTER,DEPT PHARMACOL,ROCHESTER,NY 14642. NHLBI,CHEM PHARMACOL LAB,BETHESDA,MD 20892. JOHNS HOPKINS MED INST,DEPT ANESTHESIOL & CRIT CARE MED,BALTIMORE,MD 21205. FU NIEHS NIH HHS [ES07026, ES05407] NR 30 TC 63 Z9 63 U1 0 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1407 EP 1410 DI 10.1073/pnas.88.4.1407 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700067 PM 1996342 ER PT J AU BARSONY, J MARX, SJ AF BARSONY, J MARX, SJ TI RAPID ACCUMULATION OF CYCLIC-GMP NEAR ACTIVATED VITAMIN-D RECEPTORS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE STEROID RECEPTOR; SIGNAL TRANSDUCTION; GUANYLATE CYCLASE; IMMUNOCYTOLOGY ID HUMAN-SKIN FIBROBLASTS; DEPENDENT PHOSPHORYLATION; GUANYLATE-CYCLASE; 1,25-DIHYDROXYVITAMIN-D3; HORMONE; STEROIDS; INCREASE; BINDING; CELLS; CGMP AB The mechanisms of early calcitriol (1-alpha,25-dihydroxycholecalciferol) effects, including its receptor activation process as well as its "nongenomic" effects, are poorly understood. Calcitriol causes a rapid accumulation of cGMP, dependent on the presence of normal vitamin D receptors (VDRs). We recently developed an immunocytology method based on rapid microwave fixation suitable to detect the locations of agonist-induced intracellular cGMP accumulation. With the same technique we found that calcitriol induces stepwise and rapid reorganization of VDRs. Here we used this technique to study the subcellular compartmentalization of cGMP accumulation after exposure of cells to various steroid-related agonists and to study the spatial relationship between cGMP accumulation and VDRs. Calcitriol (10 nM) within 15 sec caused clumping of VDRs and accumulation of cGMP around VDR clumps; thereafter (up to 5 min), the cGMP accumulation surrounded VDRs throughout their stepwise reorganization. In fibroblasts from subjects with mutations affecting VDR function, we found disruptions of the calcitriol-induced patterns of cGMP accumulation analogous to the disruptions of VDR reorganization. The colocalization of cGMP accumulation with reorganizing VDRs at early moments after calcitriol addition indicates transduction of the cGMP increase by VDRs inside the cell, rather than by components in the plasma membrane. Other steroid-related agonists caused compartmentalized and sequential changes in cGMP accumulation that seemed specific for each class of agonist. Our findings suggest that compartmentalized cGMP accumulation is an early and common step during activation of steroid-related receptors. RP BARSONY, J (reprint author), NIDDKD,MINERAL METAB SECT,BLDG 10,ROOM 9C-101,BETHESDA,MD 20892, USA. NR 33 TC 55 Z9 55 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1436 EP 1440 DI 10.1073/pnas.88.4.1436 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700073 PM 1847524 ER PT J AU BALZARINI, J ZHANG, H HERDEWIJN, P JOHNS, DG DECLERCQ, E AF BALZARINI, J ZHANG, H HERDEWIJN, P JOHNS, DG DECLERCQ, E TI INTRACELLULAR METABOLISM AND MECHANISM OF ANTIRETROVIRUS ACTION OF 9-(2-PHOSPHONYLMETHOXYETHYL)ADENINE, A POTENT ANTI-HUMAN-IMMUNODEFICIENCY-VIRUS COMPOUND SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE REVERSE TRANSCRIPTASE; 5-PHOSPHORIBOSYL-1-PYROPHOSPHATE SYNTHETASE ID HERPES-SIMPLEX VIRUS; CELLULAR PHARMACOLOGY; 2',3'-DIDEOXYADENOSINE; DERIVATIVES; INFECTIVITY; INHIBITOR; INVIVO; AGENT; CELLS AB 9-(2-Phosphonylmethoxyethyl)adenine (PMEA) is a potent and selective inhibitor of retrovirus (i.e., human immunodeficiency virus) replication in vitro and in vivo. Uptake of PMEA by human MT-4 cells and subsequent conversion to the mono- and diphosphorylated metabolites (PMEAp and PMEApp) are dose-dependent and occur proportionally with the initial extracellular PMEA concentrations. Adenylate kinase is unable to phosphorylate PMEA. However, 5-phosphoribosyl-1-pyrophosphate synthetase directly converts PMEA to PMEApp with a K(m) of 1.47 mM and a V(max) that is 150-fold lower than the V(max) for AMP. ATPase, 5'-phosphodiesterase, and nucleoside diphosphate kinase are able to dephosphorylate PMEApp to PMEAp, albeit to a much lower extent than the dephosphorylation of ATP. PMEApp has a relatively long intracellular half-life (16-18 hr) and has a much higher affinity for the human immunodeficiency virus-specified reverse transcriptase than for the cellular DNA polymerase-alpha (K(i)/K(m): 0.01 and 0.60, respectively). PMEApp is at least as potent an inhibitor of human immunodeficiency virus reverse transcriptase as 2', 3'-dideoxyadenosine 5'-triphosphate. Being an alternative substrate to dATP, PMEApp acts as a potent DNA chain terminator, and this may explain its anti-retrovirus activity. C1 NCI, BETHESDA, MD 20892 USA. RP BALZARINI, J (reprint author), CATHOLIC UNIV LEUVEN, REGA INST MED RES, MINDERBROEDERSSTR 10, B-3000 LOUVAIN, BELGIUM. NR 21 TC 232 Z9 236 U1 0 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1499 EP 1503 DI 10.1073/pnas.88.4.1499 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700086 PM 1705039 ER PT J AU ZHANG, YF MOSS, B AF ZHANG, YF MOSS, B TI INDUCER-DEPENDENT CONDITIONAL-LETHAL MUTANT ANIMAL VIRUSES SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE LAC OPERATOR; LAC REPRESSOR PROTEIN; VACCINIA VIRUS ID ESCHERICHIA-COLI; LAC REPRESSOR; MAMMALIAN-CELLS; FOREIGN GENES; EXPRESSION; OPERATOR; SELECTION; SYSTEM; POLYPEPTIDE; SUPPRESSOR AB Regulatory elements of the Escherichia coli lac operon were used to construct an inducer-dependent conditional-lethal mutant animal virus. The gene encoding the repressor protein of the lac operon was integrated into the vaccinia virus genome so that it was expressed constitutively, and the lac operator was inserted next to the promoter of a gene that encodes an 11-kDa virion-associated protein of unknown function. The addition of inducer to the cell culture medium provided permissive conditions for isolation of a conditional-lethal mutant virus. Under nonpermissive conditions, the isolated virus did not form plaques, and the yield was decreased by at least 1000-fold under one-step growth conditions. Transcription of the operator-controlled gene was inducer-dependent and necessary for synthesis of the 11-kDa protein. Application of this mutagenesis strategy to other viruses is discussed. RP ZHANG, YF (reprint author), NIAID,VIRAL DIS LAB,BETHESDA,MD 20892, USA. NR 28 TC 50 Z9 51 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB PY 1991 VL 88 IS 4 BP 1511 EP 1515 DI 10.1073/pnas.88.4.1511 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EY617 UT WOS:A1991EY61700089 PM 1899929 ER PT J AU DEAN, J AF DEAN, J TI THE ZONA-PELLUCIDA GENES ENCODE ESSENTIAL PROTEINS FOR MAMMALIAN FERTILIZATION AND EARLY EMBRYOGENESIS SO PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE LA English DT Review ID SPERM RECEPTOR; MOUSE OOCYTES; ZP3; GLYCOPROTEINS; INVITRO; EXPRESSION; BINDING; CULTURE RP DEAN, J (reprint author), NIDDK,CELLULAR & DEV BIOL LAB,BETHESDA,MD 20892, USA. NR 24 TC 2 Z9 2 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0037-9727 J9 P SOC EXP BIOL MED JI Proc. Soc. Exp. Biol. Med. PD FEB PY 1991 VL 196 IS 2 BP 141 EP 146 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA EV354 UT WOS:A1991EV35400003 PM 1990403 ER PT J AU GOYA, RG CASTRO, MG MEITES, J AF GOYA, RG CASTRO, MG MEITES, J TI DIFFERENTIAL EFFECT OF AGING ON SERUM LEVELS OF PROLACTIN AND ALPHA-MELANOTROPIN IN RATS SO PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE LA English DT Article ID MELANOCYTE-STIMULATING HORMONE; BETA-ENDORPHIN; PITUITARY; HYPOTHALAMUS; SECRETION; ESTROGEN; RELEASE; PLASMA; YOUNG; CELLS AB Prolactin (PRL) and alpha-melanocyte-stimulating hormone (alpha-MSH) are the only two pituitary hormones whose basal secretion is under tonic dopaminergic inhibition exerted by the hypothalamus. In the female rat, continuous exposure to estrogens is believed to depress hypothalamic dopaminergic activity and lead to the appearance of PRL-secreting pituitary adenomas during aging. Since there is no information about the impact of aging on circulating alpha-MSH levels, it was of interest to assess and compare the serum levels of PRL and alpha-MSH in male and female rats of different ages. Young (3-4 months) and old (24-25 months) male and female Sprague-Dawley rats as well as senescent (33-35 months) females were killed by decapitation between 10 AM and 1 PM, and pituitaries were immediately removed and dissected. Hormones were measured in unextracted trunk serum by radioimmunoassay. Serum PRL levels were (XBAR +/- SE), 18.4 +/- 2.0, 26.8 +/- 3.8, 19.8 +/- 2.5, 43.0 +/- 7.5, and 193.5 +/- 47.6 ng/ml for young and old males, and young, old, and senescent females, respectively. Serum alpha-MSH levels were 243.2 +/- 15.2, 252.9 +/- 24.8, 320.0 +/- 31.3, 234.7 +/- 19.1, and 374.0 +/- 29.7 pg/ml for young and old males, and young, old and senescent females, respectively. Anterior pituitary and neurointermediate lobe weights increased significantly with age in both sexes, although the change was particularly conspicuous in the females. We conclude that aging does not have a major impact on circulating alpha-MSH levels in rats and that melanotrophs probably have a greater ability than prolactotrophs to withstand age-associated alterations in central regulatory mechanisms. C1 NICHHD,NEUROCHEM & NEUROIMMUNOL LAB,BETHESDA,MD 20892. MICHIGAN STATE UNIV,DEPT PHYSIOL,E LANSING,MI 48824. FU NIA NIH HHS [AG 00416] NR 20 TC 7 Z9 7 U1 1 U2 1 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0037-9727 J9 P SOC EXP BIOL MED JI Proc. Soc. Exp. Biol. Med. PD FEB PY 1991 VL 196 IS 2 BP 218 EP 221 PG 4 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA EV354 UT WOS:A1991EV35400016 PM 1846677 ER PT J AU GRONENBORN, AM CLORE, GM AF GRONENBORN, AM CLORE, GM TI MODELING THE 3-DIMENSIONAL STRUCTURE OF THE MONOCYTE CHEMOATTRACTANT AND ACTIVATING PROTEIN MCAF/MCP-1 ON THE BASIS OF THE SOLUTION STRUCTURE OF INTERLEUKIN-8 SO PROTEIN ENGINEERING LA English DT Article DE INTERLEUKIN-8; MOLECULAR MODELING; MONOCYTE CHEMOATTRACTANT PROTEIN; NMR; SOLUTION STRUCTURE ID BOVINE PLATELET FACTOR-4; AMINO-ACID SEQUENCE; GROWTH-FACTOR; CELL-LINE; GENE; CLONING; PURIFICATION; LYMPHOCYTES; CYTOKINES; HOMOLOGY AB A model of the three-dimensional structure of the monocyte chemo-attractant and activating protein MCAF/MCP-1 is presented. The model is predicted based on the previously determined solution structure of interleukin-8 (IL-8/NAP-1) [Clore,G.M., Appella,E., Yamada,M., Matsushima,K. and Gronenborn,A.M. (1990) Biochemistry 29, 1689-1696]. Both proteins belong to a superfamily of cytokine proteins involved in cell-specific chemotaxis, host defense and the inflammatory response. The amino acid sequence identity between the two proteins is 24%. It is shown that the regular secondary structure elements of the parent structure can be retained in the modeled structure, such that the backbone hydrogen bonding pattern is very similar in the two structures. The polypeptide backbone is superimposable with an atomic r.m.s. difference of 0.9 angstrom and all side chains can be modelled by transferring the parent side chain conformation to the new structure. Thus, the deduced structure, like the parent one, is a dimer and consists of a six-stranded antiparallel beta-sheet, formed by two three-stranded Greek keys, one from each monomer, upon which lie two symmetry-related antiparallel alpha-helices, approximately 24 angstrom long and separated by approximately 14 angstrom. All amino acid sequence changes can be accommodated within the parent polypeptide framework without major rearrangements. This is borne out by the fact that the IL-8/NAP-1 and modeled MCAF/MCP-1 structures have similar non-bonding energies. These results strongly suggest that both proteins and all other members of the superfamily most likely have the same tertiary structure. Analysis of the distribution of the solvent-exposed residues can be interpreted in the context of the different receptors involved in mediating the specific responses to both proteins and suggests that the different activities of the two proteins, namely neutrophil (IL-8) versus monocyte (MCAF/MCP-1) activation and chemotaxis, reside in the specific arrangements of amino acid side chains pointing outwards from and lying in the cleft between the two exposed long alpha-helices. RP GRONENBORN, AM (reprint author), NIDDKD,CHEM PHYS LAB,BLDG 2,BETHESDA,MD 20892, USA. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 37 TC 34 Z9 35 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0269-2139 J9 PROTEIN ENG JI Protein Eng. PD FEB PY 1991 VL 4 IS 3 BP 263 EP 269 DI 10.1093/protein/4.3.263 PG 7 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA FD334 UT WOS:A1991FD33400005 PM 1857712 ER PT J AU ZIGUN, JR AF ZIGUN, JR TI TECHNICAL CONSIDERATIONS REGARDING REQUESTS FOR DISABILITY DOCUMENTATION IN THE CONTEXT OF PSYCHOTHERAPY SO PSYCHIATRIC ANNALS LA English DT Article ID SUPPLEMENTAL SECURITY INCOME; PSYCHIATRIST C1 UNIV PITTSBURGH,WESTERN PSYCHIAT INST & CLIN,SCH MED,PITTSBURGH,PA 15213. RP ZIGUN, JR (reprint author), NIMH,ST ELIZABETHS HOSP,CTR NEUROSCI,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032, USA. NR 15 TC 2 Z9 2 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0048-5713 J9 PSYCHIAT ANN JI Psychiatr. Ann. PD FEB PY 1991 VL 21 IS 2 BP 112 EP & PG 0 WC Psychiatry SC Psychiatry GA GA471 UT WOS:A1991GA47100008 ER PT J AU WEINSTEIN, EA BURNHAM, DL AF WEINSTEIN, EA BURNHAM, DL TI REDUPLICATION AND THE SYNDROME OF CAPGRAS SO PSYCHIATRY-INTERPERSONAL AND BIOLOGICAL PROCESSES LA English DT Article ID MISIDENTIFICATION; PARAMNESIA AB THE CONCEPT of reduplication has done much to break down the traditional barriers between behavioral neurology and psychiatry, and to provide a common theme for the study of the delusional misidentification syndromes. This paper describes the features of reduplication that occur in neurological patients, considers their selective and symbolic aspects, their relationship to other disturbances of behavior and to alterations in brain function, and indicates their applicability to the illusion of doubles first described by Joseph Marie Capgras. C1 USN,BETHESDA HOSP,BETHESDA,MD 20814. NIA,NEUROSCI LAB,BETHESDA,MD 20892. NR 62 TC 29 Z9 29 U1 0 U2 0 PU GUILFORD PUBLICATIONS INC PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0033-2747 J9 PSYCHIATRY JI Psychiatry-Interpers. Biol. Process. PD FEB PY 1991 VL 54 IS 1 BP 78 EP 88 PG 11 WC Psychiatry SC Psychiatry GA FG588 UT WOS:A1991FG58800009 PM 2023976 ER PT J AU ELIA, J BORCHERDING, BG RAPOPORT, JL KEYSOR, CS AF ELIA, J BORCHERDING, BG RAPOPORT, JL KEYSOR, CS TI METHYLPHENIDATE AND DEXTROAMPHETAMINE TREATMENTS OF HYPERACTIVITY - ARE THERE TRUE NONRESPONDERS SO PSYCHIATRY RESEARCH LA English DT Article DE STIMULANTS; HYPERACTIVITY; METHYLPHENIDATE; DEXTROAMPHETAMINE ID MINIMAL BRAIN-DYSFUNCTION; HYPERKINETIC-CHILDREN; STIMULANT DRUGS; CAFFEINE; SCALE AB The response to stimulant drugs of 48 boys with attention deficit/hyperactivity disorder was measured following dextroamphetamine, methylphenidate, and placebo in a double-blind crossover study. To distinguished lack of behavioral improvement from adverse drug effects, a day hospital setting and a wide dose range were used. Both drugs were highly and equally efficacious for the group as a whole, and frequently one drug or the other was superior for an individual child, or adverse effects occurred only on one of the stimulants. Only one of the 48 boys (2%) was discharged without the recommendation for continued stimulant drug treatment. "Nonresponse" appears to be extremely rare when both stimulants and a wide range of doses are given. C1 NIMH,CHILD PSYCHIAT BRANCH,BLDG 10,RM 6N240,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. FREDERICK MED CTR,FREDERICK,MD. NR 36 TC 192 Z9 193 U1 2 U2 4 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD FEB PY 1991 VL 36 IS 2 BP 141 EP 155 DI 10.1016/0165-1781(91)90126-A PG 15 WC Psychiatry SC Psychiatry GA FA398 UT WOS:A1991FA39800003 PM 2017529 ER PT J AU OREN, DA JOSEPHVANDERPOOL, JR ROSENTHAL, NE AF OREN, DA JOSEPHVANDERPOOL, JR ROSENTHAL, NE TI ADAPTATION TO DIM LIGHT IN DEPRESSED-PATIENTS WITH SEASONAL AFFECTIVE-DISORDER SO PSYCHIATRY RESEARCH LA English DT Article DE DARK ADAPTATION; DEPRESSION; EYE LIGHT; SEASONAL AFFECTIVE DISORDER ID SENSITIVITY; ANTIDEPRESSANT; PHOTOTHERAPY; DETECTABILITY; CRITERIA AB Supersensitivity to light has been suggested as a possible trait marker for manic-depressive illness. Because winter seasonal affective disorder (SAD) is associated with depressive episodes during dark winter days, the authors postulated that SAD patients would show diminished sensitivity to dim light. Dark-adaptation curves were obtained in 10 medication-free, depressed SAD patients and in 10 age- and sex-matched drug-free healthy controls. contrary to the hypothesis, patient adapted to dim light more rapidly than controls. RP OREN, DA (reprint author), NIMH,CLIN PSYCHOBIOL BRANCH,ENVIRONM PSYCHIAT SECT,BLDG 10,RM 3N22N,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 40 TC 11 Z9 11 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD FEB PY 1991 VL 36 IS 2 BP 187 EP 193 DI 10.1016/0165-1781(91)90130-H PG 7 WC Psychiatry SC Psychiatry GA FA398 UT WOS:A1991FA39800007 PM 2017533 ER PT J AU BOICE, JD PRESTON, D DAVIS, FG MONSON, RR AF BOICE, JD PRESTON, D DAVIS, FG MONSON, RR TI FREQUENT CHEST-X-RAY FLUOROSCOPY AND BREAST-CANCER INCIDENCE AMONG TUBERCULOSIS PATIENTS IN MASSACHUSETTS SO RADIATION RESEARCH LA English DT Article ID AGE 50 YEARS; FOLLOW-UP; 2ND CANCERS; WOMEN; RADIATION; MORTALITY; RISK; IRRADIATION; MAMMOGRAPHY; THERAPY C1 RADIAT EFFECTS RES FDN,DEPT STAT,HIROSHIMA 730,JAPAN. UNIV ILLINOIS,SCH PUBL HLTH,EPIDEMIOL & BIOSTAT PROGRAM,CHICAGO,IL 60680. HARVARD UNIV,SCH PUBL HLTH,DEPT EPIDEMIOL,BOSTON,MA 02115. RP BOICE, JD (reprint author), NCI,RADIAT EPIDEMIOL BRANCH,EXECUT PLAZA N,SUITE 408,BETHESDA,MD 20892, USA. FU NCI NIH HHS [N01-CP6-1006, N01-CP8-1058, N01-CP8-5604] NR 49 TC 189 Z9 193 U1 1 U2 6 PU RADIATION RESEARCH SOC PI OAK BROOK PA 2021 SPRING RD, STE 600, OAK BROOK, IL 60521 SN 0033-7587 J9 RADIAT RES JI Radiat. Res. PD FEB PY 1991 VL 125 IS 2 BP 214 EP 222 DI 10.2307/3577890 PG 9 WC Biology; Biophysics; Radiology, Nuclear Medicine & Medical Imaging SC Life Sciences & Biomedicine - Other Topics; Biophysics; Radiology, Nuclear Medicine & Medical Imaging GA EX538 UT WOS:A1991EX53800013 PM 1996380 ER PT J AU DWYER, AJ AF DWYER, AJ TI MATCHMAKING AND MCNEMAR IN THE COMPARISON OF DIAGNOSTIC MODALITIES SO RADIOLOGY LA English DT Editorial Material DE EDITORIALS; STATISTICAL ANALYSIS RP DWYER, AJ (reprint author), NIH,CTR CLIN,DEPT RADIOL,ROOM 1C660,BETHESDA,MD 20814, USA. NR 8 TC 131 Z9 132 U1 0 U2 2 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD FEB PY 1991 VL 178 IS 2 BP 328 EP 330 PG 3 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EU035 UT WOS:A1991EU03500006 PM 1987587 ER PT J AU DUNNICK, NR BUSH, WH CHOYKE, PL FERNBACH, SK HARTMAN, DS RIFKIN, MD ROUBIDOUX, MA SANDLER, CM AF DUNNICK, NR BUSH, WH CHOYKE, PL FERNBACH, SK HARTMAN, DS RIFKIN, MD ROUBIDOUX, MA SANDLER, CM TI GENITOURINARY RADIOLOGY SO RADIOLOGY LA English DT Editorial Material DE ADRENAL GLAND; CONTRAST MEDIA; KIDNEY, US STUDIES; RADIOLOGICAL-SOCIETY-OF-NORTH-AMERICA, 76TH SCIENTIFIC ASSEMBLY AND ANNUAL MEET; ULTRASOUND (US), DOPPLER STUDIES C1 VIRGINIA MASON CLIN,SEATTLE,WA. NIH,BETHESDA,MD 20892. CHILDRENS MEM HOSP,CHICAGO,IL 60614. UNIFORMED SERV UNIV HLTH SCI,BETHESDA,MD 20814. THOMAS JEFFERSON UNIV,PHILADELPHIA,PA 19107. UNIV TEXAS,SCH MED,HOUSTON,TX 77025. RP DUNNICK, NR (reprint author), DUKE UNIV,MED CTR,DURHAM,NC 27710, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD FEB PY 1991 VL 178 IS 2 BP 600 EP 601 PG 2 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EU035 UT WOS:A1991EU03500069 ER PT J AU OPARA, EC GO, VLW AF OPARA, EC GO, VLW TI INFLUENCE OF GASTRIC-INHIBITORY POLYPEPTIDE (GIP) AND GLUCOSE ON THE REGULATION OF GLUCAGON-SECRETION BY PANCREATIC ALPHA-CELLS SO REGULATORY PEPTIDES LA English DT Article DE INCRETIN; GLUCAGON RELEASE; COUNTERREGULATION; ISOLATED ISLET ID DEPENDENT DIABETES-MELLITUS; INSULIN-SECRETION; RAT; CHOLECYSTOKININ; RELEASE; STIMULATION; RESPONSES; ISLETS; MICE AB The effects of glucose and GIP on glucagon secretion were studied in perifused microdissected murine pancreatic islets. Glucagon levels were determined in effluent samples collected at 1-min intervals by radioimmunoassay using the glucagon-specific antibody, 30 K. There was no significant difference in the total amount (7740 +/- 212 pg vs 8630 +/- 36 pg, n = 10) of glucagon secreted over a 20 min period when the glucose concentration was alternately shifted between 5.5 mM and 11.1 mM, respectively. However, 22.2 mM glucose profoundly suppressed glucagon secretion. The suppressive effect of high glucose on glucagon release was partially, yet significantly, reversed by the presence of GIP, as glucagon secretion increased from a non-detectable level at 22.2 mM glucose alone to 10,175 +/- 145 pg, n = 10 (P < 0.01). The glucagonotropic effect of GIP was dose-dependent in the range of 2.10(-9)-2.10(-7) M, at 11.1 mM glucose. Our data show that GIP is able to substantially reverse the suppressive effect of a high glucose load on glucagon secretion. C1 NIDDKD,DIV DIGEST DIS & NUTR,BETHESDA,MD. NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD. NR 24 TC 8 Z9 8 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-0115 J9 REGUL PEPTIDES JI Regul. Pept. PD FEB 1 PY 1991 VL 32 IS 2 BP 65 EP 73 DI 10.1016/0167-0115(91)90035-F PG 9 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA EY260 UT WOS:A1991EY26000001 PM 2034823 ER PT J AU GAZZINELLI, RT AF GAZZINELLI, RT TI NATURAL ANTI-GAL ANTIBODIES PREVENT, RATHER THAN CAUSE, AUTOIMMUNITY IN HUMAN CHAGAS-DISEASE SO RESEARCH IN IMMUNOLOGY LA English DT Article RP GAZZINELLI, RT (reprint author), NIAID,DEPT PARASIT DIS,BLDG 4,ROOM 126,BETHESDA,MD 20892, USA. NR 18 TC 10 Z9 11 U1 0 U2 1 PU EDITIONS SCIENTIFIQUES ELSEVIER PI PARIS CEDEX 15 PA 141 RUE JAVEL, 75747 PARIS CEDEX 15, FRANCE SN 0923-2494 J9 RES IMMUNOL JI Res. Immunol. PD FEB PY 1991 VL 142 IS 2 BP 164 EP 167 DI 10.1016/0923-2494(91)90031-D PG 4 WC Immunology SC Immunology GA FM371 UT WOS:A1991FM37100016 PM 1907755 ER PT J AU TARLETON, RL RIBEIRODOSSANTOS, R PIRMEZ, C SAVINO, W HONTEBEYRIEJOSKOWICZ, M MINOPRIO, P RUSSO, M STAROBINAS, N ARNHOLDT, CV SCHARFSTEIN, J PETRY, K VANVOORHIS, WC LEVIN, M GAZZINELLI, RT GAZZINELLI, G BRENER, Z AF TARLETON, RL RIBEIRODOSSANTOS, R PIRMEZ, C SAVINO, W HONTEBEYRIEJOSKOWICZ, M MINOPRIO, P RUSSO, M STAROBINAS, N ARNHOLDT, CV SCHARFSTEIN, J PETRY, K VANVOORHIS, WC LEVIN, M GAZZINELLI, RT GAZZINELLI, G BRENER, Z TI CHAGAS-DISEASE - TRYPANOSOMA-CRUZI VS THE HOST IMMUNE-SYSTEM - 36TH FORUM IN IMMUNOLOGY - DISCUSSION SO RESEARCH IN IMMUNOLOGY LA English DT Discussion DE CHAGAS DISEASE, TRYPANOSOMA-CRUZI, LYMPHOCYTE-T, AUTOIMMUNITY, CARDITIS; FORUM ID ANTIBODIES; INFECTION; INTERFERON; PROTECTION; ANTIGENS; MOUSE; MICE C1 UNIV SAO PAULO, DEPT IMUNOL, SAO PAULO, BRAZIL. INST PASTEUR, DEPT IMMUNOL, UNITE IMMUNOPARASITOL, F-75724 PARIS 15, FRANCE. UNIV SAO PAULO, SCH MED, DEPT PARASITOL, RIBEIRAO PRETO, BRAZIL. FIOCRUZ MS, INST OSWALDO CRUZ, EVANDRO CHAGAS HOSP, DEPT IMMUNOL, BR-21045 RIO DE JANEIRO, BRAZIL. UNIV SAO PAULO, INST BIOMED SCI, DEPT IMMUNOL, BR-05508 SAO PAULO, BRAZIL. UNIV FED RIO DE JANEIRO, INST BIOFIS CARLOS CHAGAS FILHO, CCS LAB, IMUNOL MOLEC, BR-21944 RIO DE JANEIRO, BRAZIL. UNIV BORDEAUX 2, DEPT IMMUNOL & BIOL PARASITAIRE, F-33076 BORDEAUX, FRANCE. UNIV WASHINGTON, DEPT MED, DIV INFECT DIS, SEATTLE, WA 98195 USA. INST INVEST INGN GENET & BIOL MOLEC, RA-1428 BUENOS AIRES, ARGENTINA. NIAID, DEPT PARASIT DIS, BETHESDA, MD 20892 USA. FIOCRUZ MS, CTR PESQUISAS RENE RACHOU, BR-30190 BELO HORIZONTE, MG, BRAZIL. RP TARLETON, RL (reprint author), UNIV GEORGIA, 724 BIOL SCI BLDG, ATHENS, GA 30602 USA. RI Pirmez, Claude/K-8579-2012; Russo, Momtchilo/D-8614-2012; Starobinas, Nancy/B-9178-2015 OI Pirmez, Claude/0000-0002-7443-0455; NR 26 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 23 RUE LINOIS, 75724 PARIS, FRANCE SN 0923-2494 J9 RES IMMUNOL JI Res. Immunol. PD FEB PY 1991 VL 142 IS 2 BP 170 EP 181 PG 12 WC Immunology SC Immunology GA FM371 UT WOS:A1991FM37100018 ER PT J AU TRUN, NJ GOTTESMAN, S AF TRUN, NJ GOTTESMAN, S TI CHARACTERIZATION OF ESCHERICHIA-COLI MUTANTS WITH ALTERED PLOIDY SO RESEARCH IN MICROBIOLOGY LA English DT Article; Proceedings Paper CT WORKSHOP ON THE BACTERIAL CELL CYCLE : STRUCTURAL AND MOLECULAR ASPECTS CY OCT 01-04, 1990 CL COLLONGES ROUGE, FRANCE SP EUROPEAN MOLEC BIOL ORG DE ESCHERICHIA-COLI; CAMPHOR; RESISTANCE; PLOIDY; MUTANT; RECA; DAM; CHARACTERIZATION ID CHROMOSOME-REPLICATION; CELL-DIVISION; DNA; TRANSPOSITION; TERMINATION AB We describe the isolation and characterization of new mutants in the cell cycle of Escherichia coli. The mutants were selected as gain of function mutants that are able to maintain more than the normal number of chromosomes. Our increased ploidy mutants were isolated as resistant to camphor vapours, which selects for cells with more chromosomes than normal. The mutants (called mbr for moth-ball-resistant) map to four chromosomal locations: mbrA at 68 min; mbrB at 88.5 min; mbrC at 89.5 min; and mbrD at 90 min. To investigate the nature of these cell cycle mutants, we have coupled them with defects in recA, to test for induction of the SOS response, and dam, to determine if methylation is required for mbr function. Based on the results of these and other tests, we have made a preliminary placement of the mbr mutants within the context of the cell cycle. mbrA mutations appear to be defective in the coupling of the DNA replication cycle to the cell division cycle, and as such, may define a new link between the two processes. mbrB does not seem to be able to coordinate the cell cycle and the growth rate of the cell. mbrC appears to be defective in partitioning of chromosomes. mbrD, which may be allelic to rpoB (a subunit of RNA polymerase), appears to be defective in either chromosomal partitioning or the later stages of DNA replication. RP TRUN, NJ (reprint author), NCI,MOLEC BIOL LAB,BLDG 37,ROOM 4B03,BETHESDA,MD 20892, USA. NR 22 TC 7 Z9 7 U1 0 U2 0 PU EDITIONS SCIENTIFIQUES ELSEVIER PI PARIS CEDEX 15 PA 141 RUE JAVEL, 75747 PARIS CEDEX 15, FRANCE SN 0923-2508 J9 RES MICROBIOL JI Res. Microbiol. PD FEB-APR PY 1991 VL 142 IS 2-3 BP 195 EP 200 DI 10.1016/0923-2508(91)90030-E PG 6 WC Microbiology SC Microbiology GA FM035 UT WOS:A1991FM03500014 PM 1925019 ER PT J AU BRENDLER, TG ABELES, AL REAVES, LD AUSTIN, SJ AF BRENDLER, TG ABELES, AL REAVES, LD AUSTIN, SJ TI UNIQUE SEQUENCE REQUIREMENTS FOR THE P1 PLASMID REPLICATION ORIGIN SO RESEARCH IN MICROBIOLOGY LA English DT Article; Proceedings Paper CT WORKSHOP ON THE BACTERIAL CELL CYCLE : STRUCTURAL AND MOLECULAR ASPECTS CY OCT 01-04, 1990 CL COLLONGES ROUGE, FRANCE SP EUROPEAN MOLEC BIOL ORG DE P1; PLASMID; REPLICATION; GENETIC ANALYSIS ID ESCHERICHIA-COLI; DNA; PROTEIN; INITIATION; CLONING; REPA; GENE; METHYLASE AB We have carried out a detailed genetic analysis of the P1 plasmid replication origin and shown that it has four major structural requirements: the DnaA box, a series of five 7-base pair (bp) repeats, a GC-rich spacer and five 19-bp repeats that bind the P1 RepA protein. The origin requires the DnaA protein and its recognition sequence (the DnaA box). However, although five boxes are present in two separate blocks in the wild type, just one, placed either to the left or right of the core origin sequences, is sufficient for function as long as the box conforms exactly to the proposed consensus. Each of the five 7-bp repeats that constitute the core of the origin is required; mutations within any of the first six base pairs can block origin function. The required bases include, but are not limited to, those constituting dam methylation sites. Also essential is a 39-bp GC-rich sequence. We show this to be a spacer of critical length that separates the 7-bp repeats from the last required region; a series of 19-bp repeats that bind the P1 RepA initiator protein. RP BRENDLER, TG (reprint author), NCI,FREDERICK CANC RES FAC,ABL,BASIC RES PROGRAM,CHROMOSOME BIOL LAB,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74101] NR 25 TC 3 Z9 3 U1 0 U2 0 PU EDITIONS SCIENTIFIQUES ELSEVIER PI PARIS CEDEX 15 PA 141 RUE JAVEL, 75747 PARIS CEDEX 15, FRANCE SN 0923-2508 J9 RES MICROBIOL JI Res. Microbiol. PD FEB-APR PY 1991 VL 142 IS 2-3 BP 209 EP 216 DI 10.1016/0923-2508(91)90032-6 PG 8 WC Microbiology SC Microbiology GA FM035 UT WOS:A1991FM03500016 PM 1925021 ER PT J AU MICHAELS, FH BANKS, KL REITZ, MS AF MICHAELS, FH BANKS, KL REITZ, MS TI LESSONS FROM CAPRINE AND OVINE RETROVIRUS INFECTIONS SO RHEUMATIC DISEASE CLINICS OF NORTH AMERICA LA English DT Article ID ARTHRITIS-ENCEPHALITIS-VIRUS; HUMAN IMMUNODEFICIENCY VIRUS; TUMOR NECROSIS FACTOR; LONG TERMINAL REPEAT; VIRAL MESSENGER-RNA; NUCLEOTIDE-SEQUENCE; ACQUIRED IMMUNODEFICIENCY; MONONUCLEAR PHAGOCYTES; VISNA VIRUSES; HTLV-III C1 WASHINGTON STATE UNIV,COLL VET MICROBIOL & PATHOL,PULLMAN,WA 99164. RP MICHAELS, FH (reprint author), NCI,TUMOR CELL BIOL LAB,BLDG 37,ROOM 6D27,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 74 TC 14 Z9 14 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0889-857X J9 RHEUM DIS CLIN N AM JI Rheum. Dis. Clin. North Am. PD FEB PY 1991 VL 17 IS 1 BP 5 EP 23 PG 19 WC Rheumatology SC Rheumatology GA FB571 UT WOS:A1991FB57100003 PM 1645879 ER PT J AU GORDEN, P AF GORDEN, P TI CYSTIC-FIBROSIS RESEARCH SO SCIENCE LA English DT Letter RP GORDEN, P (reprint author), NIDDKD,BETHESDA,MD 20892, USA. NR 1 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD FEB 1 PY 1991 VL 251 IS 4993 BP 500 EP 500 DI 10.1126/science.1990423 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EV459 UT WOS:A1991EV45900013 PM 1990423 ER PT J AU GREM, JL AF GREM, JL TI CURRENT TREATMENT APPROACHES IN COLORECTAL-CANCER SO SEMINARS IN ONCOLOGY LA English DT Article ID POSTOPERATIVE ADJUVANT CHEMOTHERAPY; CALIFORNIA-ONCOLOGY-GROUP; HIGH-DOSE LEUCOVORIN; LARGE BOWEL-CANCER; RANDOMIZED TRIAL; SEQUENTIAL METHOTREXATE; COLON CANCER; CARCINOMA; FLUOROURACIL; THERAPY RP GREM, JL (reprint author), NCI,CLIN ONCOL PROGRAM,MED BRANCH,BLDG 10,ROOM 12N226,BETHESDA,MD 20892, USA. NR 44 TC 26 Z9 26 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0093-7754 J9 SEMIN ONCOL JI Semin. Oncol. PD FEB PY 1991 VL 18 IS 1 SU 1 BP 17 EP 26 PG 10 WC Oncology SC Oncology GA EY593 UT WOS:A1991EY59300003 PM 1992527 ER PT J AU CHAUDHURI, G HEYMANN, MA IGNARRO, LJ LONGO, LD MCNELLIS, D MITCHELL, MD VANHOUTTE, PM AF CHAUDHURI, G HEYMANN, MA IGNARRO, LJ LONGO, LD MCNELLIS, D MITCHELL, MD VANHOUTTE, PM TI RESEARCH RECOMMENDATIONS AND CONCLUSIONS FROM THE NATIONAL INSTITUTES OF CHILD HEALTH AND HUMAN-DEVELOPMENT CONFERENCE ON ENDOTHELIUM-DERIVED VASOACTIVE SUBSTANCES IN PERINATAL RESEARCH SO SEMINARS IN PERINATOLOGY LA English DT Editorial Material C1 NICHHD,EPN,ROOM 643,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. RI Mitchell, Murray/A-8639-2010 OI Mitchell, Murray/0000-0002-6167-7176 NR 0 TC 2 Z9 2 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0146-0005 J9 SEMIN PERINATOL JI Semin. Perinatol. PD FEB PY 1991 VL 15 IS 1 BP 2 EP 3 PG 2 WC Obstetrics & Gynecology; Pediatrics SC Obstetrics & Gynecology; Pediatrics GA EY468 UT WOS:A1991EY46800002 PM 2063226 ER PT J AU JOHNSON, MD AF JOHNSON, MD TI GENES RELATED TO SPERMATOGENESIS - MOLECULAR AND CLINICAL ASPECTS SO SEMINARS IN REPRODUCTIVE ENDOCRINOLOGY LA English DT Article RP JOHNSON, MD (reprint author), NICHHD,MAMMALIAN GENES & DEV LAB,HUMAN GENET BRANCH,BUILDING 6,ROOM 3A-16,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU THIEME MEDICAL PUBL INC PI NEW YORK PA 381 PARK AVE SOUTH, NEW YORK, NY 10016 SN 0734-8630 J9 SEMIN REPROD ENDOCR JI Semin. Reprod. Endocrinol. PD FEB PY 1991 VL 9 IS 1 BP 72 EP 86 DI 10.1055/s-2007-1019396 PG 15 WC Endocrinology & Metabolism; Obstetrics & Gynecology; Reproductive Biology SC Endocrinology & Metabolism; Obstetrics & Gynecology; Reproductive Biology GA EV844 UT WOS:A1991EV84400010 ER PT J AU BAIRD, DD WEINBERG, CR WILCOX, AJ MCCONNAUGHEY, DR AF BAIRD, DD WEINBERG, CR WILCOX, AJ MCCONNAUGHEY, DR TI USING THE RATIO OF URINARY ESTROGEN AND PROGESTERONE METABOLITES TO ESTIMATE DAY OF OVULATION SO STATISTICS IN MEDICINE LA English DT Article AB We have developed a method of estimating day of ovulation using urinary ovarian hormone data. The method identifies a day of luteal transition that occurs at the shift from production of follicular oestrogen to luteal progesterone. The algorithm for identifying this shift was evaluated and judged better than specified alternatives in that it resulted in (1) a high concordance between the day of luteal transition and peaks in urinary luteinizing hormone (LH) for cycles with well-defined peaks, (2) a low variance in the length of the luteal phase of the menstrual cycle, which presumably reflects a low measurement error in estimating day of ovulation, and (3) a high proportion of cycles for which an approximate day of ovulation could be determined. To validate the new algorithm, it was applied to an independent data set. The algorithm identified a day of luteal transition in 88 per cent of these cycles, and the identified day occurred within two days of the urinary LH peak for all of the cycles with clear LH peaks. Determination of the day of luteal transition to estimate ovulation requires only first-morning urine specimens, requires no correction for day-to-day variations in urine concentration, and can be applied to a mid-cycle window of data. RP BAIRD, DD (reprint author), NIEHS,DIV BIOMETRY & RISK ASSESSMENT,RES TRIANGLE PK,NC 27709, USA. OI Baird, Donna/0000-0002-5544-2653 NR 0 TC 111 Z9 111 U1 1 U2 9 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0277-6715 J9 STAT MED JI Stat. Med. PD FEB PY 1991 VL 10 IS 2 BP 255 EP 266 DI 10.1002/sim.4780100209 PG 12 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA EW847 UT WOS:A1991EW84700008 PM 2052803 ER PT J AU BASILE, AS AF BASILE, AS TI THE CONTRIBUTION OF ENDOGENOUS BENZODIAZEPINE RECEPTOR LIGANDS TO THE PATHOGENESIS OF HEPATIC-ENCEPHALOPATHY SO SYNAPSE LA English DT Article DE BENZODIAZEPINE RECEPTORS; FULMINANT HEPATIC FAILURE; DIAZEPAM; MASS SPECTROSCOPY ID RAT-BRAIN; GABA RECEPTORS; RABBIT MODEL; BASIC DRUGS; DIAZEPAM; BINDING; COMPLEX; FLUMAZENIL; MOLECULES; FAILURE AB The involvement of the gamma-aminobutyric acid(A) (GABA(A)) receptor complex in the pathogenesis of hepatic encephalopathy (HE) was examined in galactosamine-treated rabbits with HE caused by fulminant hepatic failure. Radioligand binding to the constituent components of the GABA(A) receptor complex was unchanged in rabbits with HE. However, partially purified extracts from encephalopathic rabbit brain were approximately three times more potent in inhibiting [H-3]Ro 15-1788 binding to benzodiazepine (BZ) receptors than extracts from control rabbits. The inhibition of radioligand binding to the BZ receptor produced by these extracts was competitive and reversible and was significantly enhanced by GABA. Further purification of these extracts by high-performance liquid chromatography (HPLC) indicated that the inhibitory activity was localized in several peaks, some of which had retention times corresponding to 1,4-benzodiazepine standards. The presence of diazepam in these extracts was confirmed using mass spectroscopy. Both mass spectroscopic and radiometric techniques demonstrated that the concentration of diazepam in brain extracts from encephalopathic rabbits was approximately 4 times greater than control extracts. These findings link the presence of BZ receptor agonists to the development of a neuropathological condition, thereby providing a rational basis for the use of BZ receptor antagonists in the management of HE in man. RP BASILE, AS (reprint author), NIDDK,NEUROSCI LAB,NEUROBIOL SECT,BLDG 8,ROOM 111,BETHESDA,MD 20892, USA. NR 51 TC 27 Z9 27 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0887-4476 J9 SYNAPSE JI Synapse PD FEB PY 1991 VL 7 IS 2 BP 141 EP 150 DI 10.1002/syn.890070208 PG 10 WC Neurosciences SC Neurosciences & Neurology GA EU633 UT WOS:A1991EU63300007 PM 1849322 ER PT J AU ABBOTT, BD BIRNBAUM, LS AF ABBOTT, BD BIRNBAUM, LS TI TCDD EXPOSURE OF HUMAN EMBRYONIC PALATAL SHELVES IN ORGAN-CULTURE ALTERS THE DIFFERENTIATION OF MEDIAL EPITHELIAL-CELLS SO TERATOLOGY LA English DT Article ID EPIDERMAL GROWTH-FACTOR; MOUSE DEVELOPMENT; SECONDARY PALATE; RETINOIC ACID; FACTOR-ALPHA; FACTOR-BETA; EGF RECEPTORS; EXPRESSION; 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN; FUSION AB The highly toxic, polychlorinated aromatic compound 2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) occurs as a contaminant throughout the environment. Epidemiology studies of populations accidentally exposed to TCDD have failed to identify TCDD as a human teratogen, but these studies are limited by the small numbers of exposed pregnancies and imprecise estimates of exposure. TCDD is highly teratogenic in mice, inducing cleft palate and hydronephrosis. TCDD exposure in vivo of embryonic mice alters the differentiation and expression of growth factors in the medial epithelial palatal cells. These alterations also occur in rat and mouse palates exposed to TCDD in organ culture. In the present study, human embryonic palatal shelves were cultured in the rodent organ culture system. In order to achieve in vitro the developmental stage at which fusion would normally occur, GD 52 shelves were cultured for 4 days, GD 53 shelves were cultured for 3 days, and GD 54 shelves were cultured for 3 days. Three of four palatal shelves exposed to 5 x 10(-11) M TCDD were identical to their homologous controls (right shelf cultured with control medium; left shelf cultured with TCDD-containing medium). TCDD at 1 x 10(-11) M produced cytotoxicity detected by transmission electron microscopy (TEM). Exposure to 1 x 10(-8) M TCDD resulted in continued incorporation of thymidine ([H-3]-TdR detected autoradiographically) by palatal medial cells, failure of the medial peridermal cells to degenerate as observed by scanning electron microscopy (SEM), and differentiation into a stratified, squamous epithelium. These alterations are identical to those induced by TCDD in vitro in rat and mouse palatal cells. The main difference between these species is the level of TCDD required to elicit the responses. Cultured mouse palates respond to 5 x 10(-11) M TCDD with altered medial cell differentiation, and 1 x 10(-10) M TCDD is cytotoxic. The rat shelves respond with altered differentiation at 1 x 10(-8) M cytotoxicity at 1 x 10(-7) M. All the human shelves respond at 1 x 10(-8) M TCDD with altered differentiation, 1 out of 4 responded at 5 x 10(-11) M, and cytotoxicity occurred at 1 x 10(-7) M. The present data suggest human embryonic palates are less sensitive than those of the C57BL/6N mouse, and that exposure to high levels of TCDD would be required to elicit altered differentiation in the palatal shelf. C1 NIEHS,NATL TOXICOL PROGRAM,SYST TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. NR 45 TC 36 Z9 39 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0040-3709 J9 TERATOLOGY JI Teratology PD FEB PY 1991 VL 43 IS 2 BP 119 EP 132 DI 10.1002/tera.1420430205 PG 14 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA EV805 UT WOS:A1991EV80500004 PM 2014478 ER PT J AU BANKS, YB BIRNBAUM, LS AF BANKS, YB BIRNBAUM, LS TI ABSORPTION OF 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN (TCDD) AFTER LOW-DOSE DERMAL EXPOSURE SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID POLYCHLORINATED DIBENZOFURANS; PAPER-MILLS; PULP; PHARMACOKINETICS; DISPOSITION; SINGLE; PCDFS; RATS; FATE C1 NIEHS,EXPTL TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. RP BANKS, YB (reprint author), US EPA,HLTH EFFECTS RES LAB,MD 51,RES TRIANGLE PK,NC 27711, USA. NR 19 TC 22 Z9 23 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD FEB PY 1991 VL 107 IS 2 BP 302 EP 310 DI 10.1016/0041-008X(91)90210-6 PG 9 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EW195 UT WOS:A1991EW19500009 PM 1994512 ER PT J AU TREINEN, KA CHAPIN, RE AF TREINEN, KA CHAPIN, RE TI DEVELOPMENT OF TESTICULAR LESIONS IN F344 RATS AFTER TREATMENT WITH BORIC-ACID SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID PENTYL PHTHALATE; LATE SPERMATIDS; TESTIS; SPERMATOGENESIS; 2,5-HEXANEDIONE; DISRUPTION; EXPOSURE; ANDROGEN; AGENTS RP TREINEN, KA (reprint author), NIEHS,NATL TOXICOL PROGRAM,NATL TOXICOL PROGRAM,DEV & REPROD TOXICOL GRP,POB 12233,RES TRIANGLE PK,NC 27709, USA. OI Chapin, Robert/0000-0002-5997-1261 FU NIEHS NIH HHS [N01-ES-75199] NR 24 TC 58 Z9 58 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD FEB PY 1991 VL 107 IS 2 BP 325 EP 335 DI 10.1016/0041-008X(91)90212-W PG 11 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EW195 UT WOS:A1991EW19500011 PM 1994514 ER PT J AU KUTTY, RK SANTOSTASI, G HORNG, J KRISHNA, G AF KUTTY, RK SANTOSTASI, G HORNG, J KRISHNA, G TI MPTP-INDUCED ATP DEPLETION AND CELL-DEATH IN NEUROBLASTOMAXGLIOMA HYBRID NG 108-15 CELLS - PROTECTION BY GLUCOSE AND SENSITIZATION BY TETRAPHENYLBORATE SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID 1-METHYL-4-PHENYLPYRIDINIUM ION MPP+; DESORPTION MASS-SPECTROMETRY; MONOAMINE-OXIDASE; ISOLATED HEPATOCYTES; INDUCED PARKINSONISM; RAT HEPATOCYTES; 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE; MITOCHONDRIA; NEUROTOXIN; INHIBITION RP KUTTY, RK (reprint author), NHLBI,CHEM PHARMACOL LAB,DRUG TISSUE INTERACT SECT,BETHESDA,MD 20892, USA. NR 36 TC 27 Z9 27 U1 1 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD FEB PY 1991 VL 107 IS 2 BP 377 EP 388 DI 10.1016/0041-008X(91)90217-3 PG 12 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EW195 UT WOS:A1991EW19500016 PM 1994518 ER PT J AU BLAIR, PC THOMPSON, MB WILSON, RE ESBER, HH MARONPOT, RR AF BLAIR, PC THOMPSON, MB WILSON, RE ESBER, HH MARONPOT, RR TI CORRELATION OF CHANGES IN SERUM ANALYTES AND HEPATIC HISTOPATHOLOGY IN RATS EXPOSED TO CARBON-TETRACHLORIDE SO TOXICOLOGY LETTERS LA English DT Article DE CARBON TETRACHLORIDE; HEPATOTOXICITY; HEPATOCYTE NECROSIS; FATTY CHANGE; CLINICAL PATHOLOGY ID ALKALINE-PHOSPHATASE; BILE-ACIDS; DISEASE; LIVER; CHOLESTASIS; OBSTRUCTION AB Clinical pathology data can significantly contribute to the characterization of a disease process if suitable time points for sample collection are chosen and combined with the measurement of biochemical analytes that are sensitive and specific for damage to a potential target organ. Using a well-defined model for hepatotoxicity, we correlated histopathological lesions in the liver with changes in selected serum analytes. Groups of Fischer-344 rats were treated with carbon tetrachloride (280 mg/kg in corn oil) for 1, 2, 4, 6, 8 or 10 days. Subgroups were allowed to recover for 1, 5 or 8 days, at which time blood and liver specimens were collected. Histologically, necrosis was detected in livers from rats treated for 1 and 2 days and allowed to recover for 1 day. This was followed by generalized fatty change in animals treated for longer periods. The maximum severity of fatty change occurred 7-12 days (total experimental time). A sharp rise and fall (48 h) in cytosolic enzyme activities were seen in serum. This preceded gradual increases in all analytes measured which eventually peaked at 9-11 days (total experimental time). The pattern seen in biochemical analytes paralled the development of marked fatty change. We discuss relationships between the histologic and biochemical findings and conclude that appropriate clinical biochemistry measurements in a toxicology experiment can provide valuable mechanistic information. C1 NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709. EG & G MASON RES INST,WORCESTER,MA 01608. RP BLAIR, PC (reprint author), BURROUGHS WELLCOME CO,MED BIOCHEM,3030 CORNWALLIS RD,RES TRIANGLE PK,NC 27709, USA. NR 21 TC 10 Z9 10 U1 1 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0378-4274 J9 TOXICOL LETT JI Toxicol. Lett. PD FEB PY 1991 VL 55 IS 2 BP 149 EP 159 DI 10.1016/0378-4274(91)90129-T PG 11 WC Toxicology SC Toxicology GA EZ226 UT WOS:A1991EZ22600002 PM 1998203 ER PT J AU GHANAYEM, BI MARONPOT, RR MATTHEWS, HB AF GHANAYEM, BI MARONPOT, RR MATTHEWS, HB TI EFFECTS OF SULFHYDRYL MODULATION ON ETHYL ACRYLATE-INDUCED FORESTOMACH TOXICITY SO TOXICOLOGY LETTERS LA English DT Article DE ETHYL ACRYLATE; ETHYL ACRYLATE-INDUCED FORESTOMACH TOXICITY; INDIGENOUS SULFHYDRYL MODULATION; CYSTEAMINE; DIETHYLMALEATE ID INDUCED GASTRIC TOXICITY; BUTYLATED HYDROXYANISOLE; RATS; CARCINOGENESIS; METABOLISM; LESIONS; BHA AB Acute administration of a single dose of ethyl acrylate (EA) to F344 rats by gavage caused time- and dose-dependent forestomach edema. Evidence from our laboratory and others suggested that EA is hydrolyzed to acrylic acid (AA) and ethanol both in vivo and in vitro. The major metabolites detected in the urine of rats treated with EA were derivatives of the glutathione conjugates of EA and AA. The current work was undertaken to investigate the effects of sulfhydryl-depleting agents (diethylmaleate and fasting) and sulfhydryl-containing agents (cysteine and cysteamine) on EA-induced forestomach edema. Results presented in this report revealed that pretreatment of rats with sulfhydryl-containing chemicals such as cysteine or cysteamine has potentiated EA-induced forestomach edema. In contrast, depletion of indigenous sulfhydryls by fasting of rats or pretreatment with diethylmaleate (DEM) protected against EA-induced forestomach edema. Furthermore, repetitive daily administration of EA by gavage induced mucosal forestomach hyperplasia. Co-administration of cysteamine and EA resulted in a significant enhancement of the severity of EA-induced forestomach mucosal hyperplasia. In conclusion, current data suggest that modulation of indigenous sulfhydryls play a role in EA-induced forestomach toxicity; however, the exact mechanism underlying this role remains to be characterized. RP GHANAYEM, BI (reprint author), NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709, USA. NR 12 TC 5 Z9 5 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0378-4274 J9 TOXICOL LETT JI Toxicol. Lett. PD FEB PY 1991 VL 55 IS 2 BP 215 EP 221 DI 10.1016/0378-4274(91)90136-T PG 7 WC Toxicology SC Toxicology GA EZ226 UT WOS:A1991EZ22600009 PM 1998209 ER PT J AU SACHS, DH AF SACHS, DH TI BASIC SCIENCE HIGHLIGHTS AT THE 1990 CONGRESS SO TRANSPLANTATION PROCEEDINGS LA English DT Editorial Material ID LYMPHOCYTES; THYMUS RP SACHS, DH (reprint author), NCI,IMMUNOL BRANCH,BETHESDA,MD 20892, USA. NR 13 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 28 EP 31 PN 1 PG 4 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000011 PM 1990531 ER PT J AU ROSENBERG, AS MANIERO, TG MORSE, HC AF ROSENBERG, AS MANIERO, TG MORSE, HC TI INVIVO IMMUNOLOGICAL DEFICITS IN MICE WITH MURINE ACQUIRED-IMMUNODEFICIENCY-SYNDROME AND THE EFFECT OF LP-BM5 INFECTION ON REJECTION OF SKIN FROM INFECTED MICE SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC ID T-CELL SUBSETS; LEUKEMIA-VIRUS; INDUCTION; DISEASE C1 US FDA,CTR BIOLOG EVALUAT & RES,DIV CYTOKINE BIOL,BETHESDA,MD 20014. NIAID,IMMUNOPATHOL LAB,BETHESDA,MD 20892. NR 14 TC 16 Z9 16 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 167 EP 169 PN 1 PG 3 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000062 PM 1990505 ER PT J AU CULVER, KW OSBORNE, WRA MILLER, AD FLEISHER, TA BERGER, M ANDERSON, WF BLAESE, RM AF CULVER, KW OSBORNE, WRA MILLER, AD FLEISHER, TA BERGER, M ANDERSON, WF BLAESE, RM TI CORRECTION OF ADA DEFICIENCY IN HUMAN LYMPHOCYTES-T USING RETROVIRAL-MEDIATED GENE-TRANSFER SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC ID ADENOSINE-DEAMINASE DEFICIENCY C1 UNIV WASHINGTON,SEATTLE,WA 98195. FRED HUTCHINSON CANC RES CTR,SEATTLE,WA 98104. CASE WESTERN RESERVE UNIV,CLEVELAND,OH 44106. RP CULVER, KW (reprint author), NIH,BETHESDA,MD 20892, USA. OI Miller, Dusty/0000-0002-3736-3660 NR 4 TC 56 Z9 57 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 170 EP 171 PN 1 PG 2 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000063 PM 1846711 ER PT J AU ROSENGARD, BR OJIKUTU, CA GUZZETTA, PC SMITH, CV SUNDT, TM NAKAJIMA, K BOORSTEIN, SM HILL, GS SACHS, DH AF ROSENGARD, BR OJIKUTU, CA GUZZETTA, PC SMITH, CV SUNDT, TM NAKAJIMA, K BOORSTEIN, SM HILL, GS SACHS, DH TI RENAL-TRANSPLANTATION IN MINIATURE SWINE - PRELIMINARY EVIDENCE THAT GRAFT INFILTRATING LEUKOCYTES SUPPRESS DONOR-SPECIFIC CELL-MEDIATED LYMPHOCYTOTOXICITY IN COCULTURE SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC ID MAJOR HISTOCOMPATIBILITY COMPLEX; HOST DISEASE; T-CELLS; ALLOGRAFTS; ANTIGEN; SURVIVAL; RATS C1 NCI,IMMUNOL BRANCH,TRANSPLANTAT BIOL SECT,9000 ROCKVILLE PIKE,BLDG 10,ROOM 4B13,BETHESDA,MD 20892. FRANCIS SCOTT KEY MED CTR,DEPT PATHOL,BALTIMORE,MD. MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114. HOWARD HUGHES MED INST,BALTIMORE,MD. NR 17 TC 13 Z9 13 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 189 EP 191 PN 1 PG 3 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000072 PM 1990511 ER PT J AU NAKAJIMA, K SMITH, CV MIXON, A ROSENGARD, BR GUZZETTA, PC SPITZER, TR ECKHAUS, MA SACHS, DH AF NAKAJIMA, K SMITH, CV MIXON, A ROSENGARD, BR GUZZETTA, PC SPITZER, TR ECKHAUS, MA SACHS, DH TI THE EFFECTS OF RECOMBINANT HUMAN IL-2 IN MINIATURE SWINE SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC ID BONE-MARROW TRANSPLANTATION; MONOCLONAL-ANTIBODIES; T-CELLS; INTERLEUKIN-2; DEPLETION C1 CHILDRENS HOSP,NATL MED CTR,DEPT SURG,WASHINGTON,DC 20010. GEORGE WASHINGTON UNIV,DEPT CHILD HLTH & DEV,WASHINGTON,DC 20052. GEORGETOWN UNIV,MED CTR,VINCENT T LOMBARDI CANC RES CTR,WASHINGTON,DC 20007. NIH,DIV RES SERV,VET RESOURCES BRANCH,BETHESDA,MD 20892. RP NAKAJIMA, K (reprint author), NCI,IMMUNOL BRANCH,TRANSPLANTAT BIOL SECT,BETHESDA,MD 20892, USA. NR 16 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 248 EP 250 PN 1 PG 3 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000096 PM 1990524 ER PT J AU HIRSCH, R BLUESTONE, JA BARE, CV GRESS, RE AF HIRSCH, R BLUESTONE, JA BARE, CV GRESS, RE TI ADVANTAGES OF F(AB')2 FRAGMENTS OF ANTI-CD3 MONOCLONAL-ANTIBODY AS COMPARED TO WHOLE ANTIBODY AS IMMUNOSUPPRESSIVE AGENTS IN MICE SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC ID TUMOR NECROSIS FACTOR; T-CELL FUNCTION; OKT3; RECIPIENTS; INTERFERON; SERUM C1 UNIV CHICAGO,BEN MAY INST,CHICAGO,IL 60637. RP HIRSCH, R (reprint author), NCI,EXPTL IMMUNOL BRANCH,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 8 TC 21 Z9 21 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 270 EP 271 PN 1 PG 2 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000106 PM 1824981 ER PT J AU CARROLL, PB GONCALVES, AA BOSCHERO, AC TZAKIS, AG STARZL, TE ATWATER, I AF CARROLL, PB GONCALVES, AA BOSCHERO, AC TZAKIS, AG STARZL, TE ATWATER, I TI EFFECT OF THE IMMUNOSUPPRESSANT FK-506 ON INSULIN RELEASE FROM ADULT-RAT ISLETS OF LANGERHANS SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC ID PANCREATIC BETA-CELLS; CYCLOSPORIN-A; GLUCOSE; TRANSPLANTATION C1 UNIV PITTSBURGH,SCH MED,DEPT SURG,PITTSBURGH,PA 15261. RP CARROLL, PB (reprint author), NIDDKD,CELL BIOL & GENET LAB,BLDG 8,ROOM 326,BETHESDA,MD 20892, USA. RI Boschero, Antonio/O-7525-2014 OI Boschero, Antonio/0000-0003-3829-8570 FU NIDDK NIH HHS [R01 DK029961-19] NR 24 TC 6 Z9 6 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 337 EP 339 PN 1 PG 3 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000133 PM 1703688 ER PT J AU SUNDT, TM LEGUERN, C SMITH, CV SACHS, DH AF SUNDT, TM LEGUERN, C SMITH, CV SACHS, DH TI IDENTIFICATION OF A CD4 POLYMORPHISM IN SWINE SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC ID MONOCLONAL-ANTIBODIES; T4 C1 NCI,IMMUNOL BRANCH,TRANSPLANTAT BIOL SECT,BETHESDA,MD 20892. RP SUNDT, TM (reprint author), MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114, USA. NR 5 TC 5 Z9 5 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 419 EP 420 PN 1 PG 2 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000164 PM 1990575 ER PT J AU LEGUERN, C SHAFER, GE ALEXANDER, RC GERMANA, S GUSTAFSSON, K ELGAMIL, M SACHS, DH AF LEGUERN, C SHAFER, GE ALEXANDER, RC GERMANA, S GUSTAFSSON, K ELGAMIL, M SACHS, DH TI EXPRESSION OF SWINE CLASS-II GENES USING RECOMBINANT RETROVIRAL VECTORS SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. SWEDISH UNIV AGR SCI,UPPSALA BIOCHEM CTR,DEPT ANIM BREEDING & GENET,S-75007 UPPSALA,SWEDEN. RP LEGUERN, C (reprint author), NCI,IMMUNOL BRANCH,TRANSPLANTAT BIOL SECT,BETHESDA,MD 20892, USA. NR 8 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 427 EP 428 PN 1 PG 2 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000167 PM 1846714 ER PT J AU BOORSTEIN, SM ROSENGARD, BR SACHS, DH AF BOORSTEIN, SM ROSENGARD, BR SACHS, DH TI INVITRO STUDIES INVOLVING APC-DEPLETED RESPONDER CELLS SUGGEST THE EXISTENCE OF A CD8+ DIRECT RECOGNITION PATHWAY IN MINIATURE SWINE SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC ID SUPPRESSOR T-CELLS; CLASS-I; MONOCLONAL-ANTIBODIES; IMMUNE-RESPONSES; ANTIGEN; HISTOCOMPATIBILITY; TRANSPLANTATION; DIFFERENTIATION; CML; RAT C1 HOWARD HUGHES MED INST,BETHESDA,MD. RP SACHS, DH (reprint author), NCI,IMMUNOL BRANCH,TRANSPLANTAT BIOL SECT,9000 ROCKVILLE PIKE,BLDG 10,ROOM 4B17,BETHESDA,MD 20892, USA. NR 17 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 828 EP 829 PN 1 PG 2 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000332 PM 1825000 ER PT J AU AKSENTIJEVICH, I SHARABI, Y SUNDT, TM SACHS, DH SYKES, M AF AKSENTIJEVICH, I SHARABI, Y SUNDT, TM SACHS, DH SYKES, M TI HUMORAL TOLERANCE IN MIXED XENOGENEIC CHIMERAS PREPARED BY A NONMYELOABLATIVE CONDITIONING REGIMEN SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT 13TH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOC CY AUG 19-24, 1990 CL SAN FRANCISCO, CA SP TRANSPLANTAT SOC RP AKSENTIJEVICH, I (reprint author), NCI,IMMUNOL BRANCH,BLDG 10,ROOM 4B13,BETHESDA,MD 20892, USA. NR 3 TC 9 Z9 9 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 880 EP 882 PN 1 PG 3 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV390 UT WOS:A1991EV39000353 PM 1990712 ER PT J AU EIRAS, G SHIMIZU, Y VANSEVENTER, GA DUQUESNOY, RJ ZEEVI, A AF EIRAS, G SHIMIZU, Y VANSEVENTER, GA DUQUESNOY, RJ ZEEVI, A TI EFFECTS OF FK506 AND CYCLOSPORINE ON T-CELL ACTIVATION - INTEGRIN-MEDIATED ADHESION OF T-CELLS, PROLIFERATION, AND MATURATION OF CYTOTOXIC T-CELLS SO TRANSPLANTATION PROCEEDINGS LA English DT Article ID PEPTIDYL-PROLYL ISOMERASE; TOXIC LYMPHOCYTES-T; IMMUNOSUPPRESSANT FK506; MOLECULE-1 ICAM-1; RECEPTOR COMPLEX; PATHWAYS; FIBRONECTIN; PROTEIN; LIGAND; FK-506 C1 PRESBYTERIAN UNIV HOSP,DIV CLIN IMMUNOPATHOL,DESOTO & OHARA ST,PITTSBURGH,PA 15213. NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. FU NHLBI NIH HHS [HL 36416]; NIAID NIH HHS [AI 23467] NR 28 TC 17 Z9 17 U1 0 U2 0 PU APPLETON & LANGE PI E NORWALK PA 25 VAN ZANT ST, E NORWALK, CT 06855 SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 936 EP 939 PN 2 PG 4 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV391 UT WOS:A1991EV39100013 PM 1703357 ER PT J AU SALOMON, D BRUNSON, M VANSICKLER, J PFAFF, W HOWARD, R PETERSON, J CURRY, T THOMPSON, R SQUIERS, E AF SALOMON, D BRUNSON, M VANSICKLER, J PFAFF, W HOWARD, R PETERSON, J CURRY, T THOMPSON, R SQUIERS, E TI A RETROSPECTIVE ANALYSIS OF LATE RENAL GRAFT FUNCTION - CORRELATION WITH MEAN CYCLOSPORINE LEVELS AND LACK OF EVIDENCE FOR CHRONIC CYCLOSPORINE TOXICITY SO TRANSPLANTATION PROCEEDINGS LA English DT Article C1 UNIV FLORIDA,KIDNEY TRANSPLANT PROGRAM,DEPT MED,GAINESVILLE,FL 32611. UNIV FLORIDA,KIDNEY TRANSPLANT PROGRAM,DEPT SURG,GAINESVILLE,FL 32611. RP SALOMON, D (reprint author), NIAID,IMMUNOL LAB,CELLULAR IMMUNOL SECT,BLDG 10,ROOM 11N311,BETHESDA,MD 20892, USA. RI Salomon, Daniel/E-9380-2012 NR 0 TC 21 Z9 22 U1 0 U2 0 PU APPLETON & LANGE PI E NORWALK PA 25 VAN ZANT ST, E NORWALK, CT 06855 SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 1018 EP 1019 PN 2 PG 2 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV391 UT WOS:A1991EV39100045 PM 1989143 ER PT J AU KIRKMAN, RL SHAPIRO, ME CARPENTER, CB MCKAY, DB MILFORD, EL RAMOS, EL TILNEY, NL WALDMANN, TA ZIMMERMAN, CE STROM, TB AF KIRKMAN, RL SHAPIRO, ME CARPENTER, CB MCKAY, DB MILFORD, EL RAMOS, EL TILNEY, NL WALDMANN, TA ZIMMERMAN, CE STROM, TB TI A RANDOMIZED PROSPECTIVE TRIAL OF ANTI-TAC MONOCLONAL-ANTIBODY IN HUMAN RENAL-TRANSPLANTATION SO TRANSPLANTATION PROCEEDINGS LA English DT Article ID HUMAN T-CELLS C1 HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,BOSTON,MA 02215. NCI,METAB BRANCH,BETHESDA,MD 20892. RP KIRKMAN, RL (reprint author), HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT SURG,75 FRANCIS ST,BOSTON,MA 02115, USA. OI Shapiro, Michael/0000-0001-7491-6490 FU NIAID NIH HHS [1-AI-82512] NR 5 TC 16 Z9 17 U1 0 U2 0 PU APPLETON & LANGE PI E NORWALK PA 25 VAN ZANT ST, E NORWALK, CT 06855 SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 1066 EP 1067 PN 2 PG 2 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV391 UT WOS:A1991EV39100063 PM 1989150 ER PT J AU MULUK, SC CLERICI, M VIA, CS WIER, MR KIMMEL, PL SHEARER, GM AF MULUK, SC CLERICI, M VIA, CS WIER, MR KIMMEL, PL SHEARER, GM TI A NEW APPROACH FOR ANALYSIS OF THE MIXED LYMPHOCYTE-REACTION THAT IS PREDICTIVE FOR HUMAN RENAL-ALLOGRAFT REJECTION SO TRANSPLANTATION PROCEEDINGS LA English DT Article ID TRANSPLANTATION; CULTURE; EXPRESSION; SURVIVAL C1 NCI,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4B17,BETHESDA,MD 20892. UNIV MARYLAND,SCH MED,DEPT MED,DIV NEPHROL,BALTIMORE,MD 21201. UNIV MARYLAND,SCH MED,DEPT MED,DIV RHEUMATOL,BALTIMORE,MD 21201. UNIV MARYLAND,SCH MED,DEPT MED,DIV CLIN IMMUNOL,BALTIMORE,MD 21201. VET ADM MED CTR,RES SERV,BALTIMORE,MD 21218. GEORGETOWN UNIV,MED CTR,DEPT MED,WASHINGTON,DC 20007. NR 19 TC 8 Z9 8 U1 0 U2 0 PU APPLETON & LANGE PI E NORWALK PA 25 VAN ZANT ST, E NORWALK, CT 06855 SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD FEB PY 1991 VL 23 IS 1 BP 1274 EP 1276 PN 2 PG 3 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EV391 UT WOS:A1991EV39100147 PM 1824894 ER PT J AU BLUMENTHAL, R LOYTER, A AF BLUMENTHAL, R LOYTER, A TI RECONSTITUTED VIRAL ENVELOPES - TROJAN HORSES FOR DRUG DELIVERY AND GENE-THERAPY SO TRENDS IN BIOTECHNOLOGY LA English DT Article ID SENDAI VIRUS ENVELOPES; ADULT-RAT LIVER; HUMAN-ERYTHROCYTES; DIPHTHERIA-TOXIN; MAMMALIAN-CELLS; FUSION; DNA; EXPRESSION; ANTIBODIES; MOLECULES AB Reconstituted viral envelopes (RVEs) are formed by solubilizing intact virus in detergent and reassembling the envelope on removal of detergent. RVEs can be formed in the presence of agents that become encapsulated and can then be utilized in vitro and in vivo for drug delivery, cell destruction, transfer of membrane components, and as vectors for genetic engineering. The problems with biotechnological applications of RVEs and possible strategies for overcoming them are discussed in this article. C1 HEBREW UNIV JERUSALEM,DEPT BIOL CHEM,JERUSALEM,ISRAEL. RP BLUMENTHAL, R (reprint author), NCI,LMMB,MEMBRANE STRUCT & FUNC SECT,BETHESDA,MD 20892, USA. NR 23 TC 10 Z9 10 U1 1 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0167-7799 J9 TRENDS BIOTECHNOL JI Trends Biotechnol. PD FEB PY 1991 VL 9 IS 2 BP 41 EP 45 DI 10.1016/0167-7799(91)90184-J PG 5 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA EW608 UT WOS:A1991EW60800002 PM 1366950 ER PT J AU LAROCCA, RV COOPER, MR UHRICH, M DANESI, R WALTHER, MM LINEHAN, WM MYERS, CE AF LAROCCA, RV COOPER, MR UHRICH, M DANESI, R WALTHER, MM LINEHAN, WM MYERS, CE TI USE OF SURAMIN IN TREATMENT OF PROSTATIC-CARCINOMA REFRACTORY TO CONVENTIONAL HORMONAL MANIPULATION SO UROLOGIC CLINICS OF NORTH AMERICA LA English DT Article ID GROWTH-FACTOR RECEPTOR; SIMIAN SARCOMA-VIRUS; HTLV-III/LAV INFECTION; RED BLOOD-CELLS; TRANSFORMED CELLS; IMMUNE-RESPONSES; INHIBITION; MUCOPOLYSACCHARIDOSIS; GLYCOSAMINOGLYCAN; MECHANISM C1 NCI,CLIN ONCOL PROGRAM,BETHESDA,MD 20892. NCI,MED BRANCH,BETHESDA,MD 20892. NR 43 TC 50 Z9 50 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0094-0143 J9 UROL CLIN N AM JI Urol. Clin. N. Am. PD FEB PY 1991 VL 18 IS 1 BP 123 EP 129 PG 7 WC Urology & Nephrology SC Urology & Nephrology GA EW377 UT WOS:A1991EW37700013 PM 1992566 ER PT J AU GOODRICH, MS MELANCON, MJ DAVIS, RA LECH, JJ AF GOODRICH, MS MELANCON, MJ DAVIS, RA LECH, JJ TI THE TOXICITY, BIOACCUMULATION, METABOLISM AND ELIMINATION OF DIOCTYL SODIUM SULFOSUCCINATE DSS IN RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) SO WATER RESEARCH LA English DT Article DE RAINBOW TROUT; DIOCTYL SODIUM SULFOSUCCINATE; TOXICITY; BIOACCUMULATION; METABOLISM; EXCRETION AB The acute toxicity (LC50) of dioctyl sodium sulfosuccinate (DSS) was determined to be 28 mg/l in rainbow trout. A static non-replacement exposure of rainbow trout to C-14-labeled DSS was used to measure the bioaccumulation and elimination of DSS within four separate body compartments: blood, bile, viscera and carcass. The trout were exposed for 72 h followed by a 72 h period for depuration. Tissue samples were analyzed at 2, 4, 12, 24, 48 and 72 h during both the exposure and depuration phases of the experiment. Using scintillation counting, DSS concentrations were measured in tissues and used to calculate uptake rate constants, bioconcentration factors, elimination rate constants and half-lives of DSS elimination for each body compartment. The greatest rate of appearance of DSS was in the bile of the trout, while the slowest uptake occurred within the carcass. Elimination of DSS from the carcass and viscera of the trout was found to follow second order kinetics and elimination from the blood and bile followed first order kinetics. The evaluation of [C-14]DSS metabolism was conducted using the HPLC analysis of biliary metabolities following an intraperitoneal injection. Two major peaks were found containing C-14 that were not associated with the parent DSS peak. C1 MED COLL WISCONSIN,DEPT PHARMACOL & TOXICOL,MILWAUKEE,WI 53226. NIEHS,CTR AQUAT BIOMED,GREAT LAKES RES FACIL,MILWAUKEE,WI 53204. AMER CYANAMID CO,DEPT TOXICOL & PROD SAFETY,WAYNE,NJ 07470. NR 16 TC 12 Z9 12 U1 0 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0043-1354 J9 WATER RES JI Water Res. PD FEB PY 1991 VL 25 IS 2 BP 119 EP 124 DI 10.1016/0043-1354(91)90020-Q PG 6 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA FB020 UT WOS:A1991FB02000001 ER PT J AU YOSHIMURA, T TAKEYA, M TAKAHASHI, K AF YOSHIMURA, T TAKEYA, M TAKAHASHI, K TI MOLECULAR-CLONING OF RAT MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) AND ITS EXPRESSION IN RAT SPLEEN-CELLS AND TUMOR-CELL LINES SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID BLOOD MONONUCLEAR LEUKOCYTES; AMINO-ACID ANALYSIS; COMPETENCE GENE-JE; PERITONEAL-MACROPHAGES; GROWTH-FACTOR; PURIFICATION; SEQUENCE C1 KUMAMOTO UNIV,SCH MED,DEPT PATHOL,KUMAMOTO 860,JAPAN. RP YOSHIMURA, T (reprint author), NCI,FREDERICK CANC RES & DEV CTR,IMMUNOBIOL LAB,IMMUNOPATHOL SECT,FREDERICK,MD 21702, USA. NR 20 TC 140 Z9 143 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD JAN 31 PY 1991 VL 174 IS 2 BP 504 EP 509 DI 10.1016/0006-291X(91)91445-I PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EV749 UT WOS:A1991EV74900015 PM 1704226 ER PT J AU COOK, JC CHOCK, PB AF COOK, JC CHOCK, PB TI IMMUNOCYTOCHEMICAL LOCALIZATION OF UBIQUITIN-ACTIVATING ENZYME IN THE CELL-NUCLEUS SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID PROTEIN-DEGRADATION; HEAT-SHOCK; PROTEOLYTIC PATHWAY; CONJUGATING ENZYME; POLYPEPTIDE; MICROINJECTION; PURIFICATION; METABOLISM; COMPONENT; RECEPTOR RP COOK, JC (reprint author), NHLBI,BIOCHEM LAB,METABOL REGULAT SECT,BLDG 3,ROOM 202,BETHESDA,MD 20892, USA. NR 36 TC 20 Z9 20 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD JAN 31 PY 1991 VL 174 IS 2 BP 564 EP 571 DI 10.1016/0006-291X(91)91454-K PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EV749 UT WOS:A1991EV74900024 PM 1993053 ER PT J AU MCKEON, C PHAM, T AF MCKEON, C PHAM, T TI TRANSACTIVATION OF THE HUMAN INSULIN-RECEPTOR GENE BY THE CAAT ENHANCER BINDING-PROTEIN SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID PROMOTER REGION; TRANSCRIPTION; EXPRESSION RP MCKEON, C (reprint author), NIDKDD,DIABET BRANCH,BETHESDA,MD 20892, USA. NR 18 TC 29 Z9 30 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD JAN 31 PY 1991 VL 174 IS 2 BP 721 EP 728 DI 10.1016/0006-291X(91)91477-T PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EV749 UT WOS:A1991EV74900047 PM 1993067 ER PT J AU KIM, HY SAWAZAKI, S SALEM, N AF KIM, HY SAWAZAKI, S SALEM, N TI LIPOXYGENATION IN RAT-BRAIN SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID DOCOSAHEXAENOIC ACID; HYDROXYEICOSATETRAENOIC ACIDS; LEUKOTRIENE; INJURY RP NIAAA, DICBR, CLIN STUDIES LAB, BETHESDA, MD 20892 USA. NR 20 TC 22 Z9 22 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X EI 1090-2104 J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD JAN 31 PY 1991 VL 174 IS 2 BP 729 EP 734 DI 10.1016/0006-291X(91)91478-U PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EV749 UT WOS:A1991EV74900048 PM 1899573 ER PT J AU KOLCH, W HEIDECKER, G LLOYD, P RAPP, UR AF KOLCH, W HEIDECKER, G LLOYD, P RAPP, UR TI RAF-1 PROTEIN-KINASE IS REQUIRED FOR GROWTH OF INDUCED NIH/3T3 CELLS SO NATURE LA English DT Article ID SIGNAL TRANSDUCTION; V-RAF; ONCOGENE; PHOSPHORYLATION AB MANY growth factors regulate the cytoplasmic RAF-1 protein kinase 1-10, consistent with its having a central role in transduction of growth signals. The kinase is ubiquitously expressed 11 and can promote proliferation 12, presumably in a manner dependent on growth-factor receptors and membrane-associated oncogenes 13-15. We have now examined the dependence of serum- and TPa (12-O-tetradecanoylphorbol-13-acetate)-regulated NIH/3T3 cell growth on RAF-1 kinase to determine whether Raf-1 is essential for receptor signalling. We inhibited Raf-1 function by expressing c-raf-1 antisense RNA or kinase-defective c-raf-1 mutants. Antisense RNA for c-raf-1 interferes with proliferation of normal NIH/3T3 cells and reverts raf-transformed cells. In revertant cells, DNA replication induced by serum or TPA was eliminated or reduced proportionately to the reduction in Raf protein levels. Expression of a kinase-defective Raf-1 mutant (craf301) or a regulatory domain fragment (HCR) inhibited serum-induced NIH/3T3-cell proliferation and raf transformation even more efficiently. Inhibition by antisense RNA or craf301 blocked proliferation and transformation by Ki- and Ha-ras oncogenes. We conclude that raf functions as an essential signal transducer downstream of serum growth factor receptors, protein kinase C and ras. C1 NCI,FREDERICK CANC RES & DEV CTR,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC DYNCORP,FREDERICK,MD 21702. OI Kolch, Walter/0000-0001-5777-5016 NR 26 TC 457 Z9 460 U1 2 U2 5 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD JAN 31 PY 1991 VL 349 IS 6308 BP 426 EP 428 DI 10.1038/349426a0 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EV514 UT WOS:A1991EV51400055 PM 1992343 ER PT J AU PELLICCIA, A MARON, BJ SPATARO, A PROSCHAN, MA SPIRITO, P AF PELLICCIA, A MARON, BJ SPATARO, A PROSCHAN, MA SPIRITO, P TI THE UPPER LIMIT OF PHYSIOLOGICAL CARDIAC-HYPERTROPHY IN HIGHLY TRAINED ELITE ATHLETES SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID LEFT-VENTRICULAR HYPERTROPHY; M-MODE ECHOCARDIOGRAPHY; NON-INVASIVE EVALUATION; DOPPLER ECHOCARDIOGRAPHY; CLINICAL MANIFESTATIONS; CARDIOVASCULAR-DISEASE; DIASTOLIC FUNCTION; DISTANCE RUNNERS; PATHO-PHYSIOLOGY; CARDIOMYOPATHY AB Background. In some highly trained athletes, the thickness of the left ventricular wall may increase as a consequence of exercise training and resemble that found in cardiac diseases associated with left ventricular hypertrophy, such as hypertrophic cardiomyopathy. In these athletes, the differential diagnosis between physiologic and pathologic hypertrophy may be difficult. Methods. To address this issue, we measured left ventricular dimensions with echocardiography in 947 elite, highly trained athletes who participated in a wide variety of sports. Results. The thickest left ventricular wall among the athletes measured 16 mm. Wall thicknesses within a range compatible with the diagnosis of hypertrophic cardiomyopathy (greater-than-or-equal-to 13 mm) were identified in only 16 of the 947 athletes (1.7 percent); 15 were rowers or canoeists, and 1 was a cyclist. Therefore, the wall was greater-than-or-equal-to 13 mm thick in 7 percent of 219 rowers, canoeists, and cyclists but in none of 728 participants in 22 other sports. All athletes with walls greater-than-or-equal-to 13 mm thick also had enlarged left ventricular end-diastolic cavities (dimensions, 55 to 63 mm). Conclusions. On the basis of these data, a left-ventricular-wall thickness of greater-than-or-equal-to 13 mm is very uncommon in highly trained athletes, virtually confined to athletes training in rowing sports, and associated with an enlarged left ventricular cavity. In addition, the upper limit to which the thickness of the left ventricular wall may be increased by athletic training appears to be 16 mm. Therefore, athletes with a wall thickness of more than 16 mm and a nondilated left ventricular cavity are likely to have primary forms of pathologic hypertrophy, such as hypertrophic cardiomyopathy. C1 NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. NHLBI,BIOSTAT RES BRANCH,BETHESDA,MD 20892. RP PELLICCIA, A (reprint author), COMITATO OLIMPICO NAZL ITALIANO,INST SPORTS SCI,DEPT MED,VIA CAMPI SPORTIVI 46,I-00197 ROME,ITALY. NR 42 TC 523 Z9 529 U1 3 U2 26 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 31 PY 1991 VL 324 IS 5 BP 295 EP 301 DI 10.1056/NEJM199101313240504 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA EU922 UT WOS:A1991EU92200004 PM 1824720 ER PT J AU HAFEMEISTER, TL KEILITZ, I BANKS, SM AF HAFEMEISTER, TL KEILITZ, I BANKS, SM TI STATE COURTS AND DECISIONS ABOUT LIFE-SUSTAINING TREATMENT SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 NIH,BETHESDA,MD 20892. RP HAFEMEISTER, TL (reprint author), NATL CTR STATE COURTS,WILLIAMSBURG,VA 23187, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 31 PY 1991 VL 324 IS 5 BP 343 EP 344 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA EU922 UT WOS:A1991EU92200032 PM 1986299 ER PT J AU LAKSO, M MASAKI, R NOSHIRO, M NEGISHI, M AF LAKSO, M MASAKI, R NOSHIRO, M NEGISHI, M TI STRUCTURES AND CHARACTERIZATION OF SEX-SPECIFIC MOUSE CYTOCHROME-P-450 GENES AS MEMBERS WITHIN A LARGE FAMILY - DUPLICATION BOUNDARY AND EVOLUTION SO EUROPEAN JOURNAL OF BIOCHEMISTRY LA English DT Article ID TESTOSTERONE 16-ALPHA-HYDROXYLASE C-P-45016-ALPHA; PHENOBARBITAL-INDUCIBLE CYTOCHROME-P-450; CHROMOSOME LOCALIZATION; SEQUENCE-ANALYSIS; RAT-LIVER; DNA; CONVERSION; CLONING; CDNA; I-P-45016-ALPHA AB We characterized two female-specific testosterone 16-alpha-hydroxylase mouse cytochrome P-450 genes, 16-alpha-oh-a and 16-alpha-oh-b. Gene 16-alpha-oh-a, consisting of nine exons, is approximately 38 kbp in size. The exon sequence of this P-450 gene is identical to cDNA pf26 nucleotide sequence [Noshiro, M., Lakso, M., Kawajiri, K. & Negishi, M. (1988) Biochemistry 27, 6434-6443], which encodes female-specific testosterone 16-alpha-hydroxylase regulated by the murine Rip locus. Gene 16-alpha-oh-b, containing nine exons with the same junctions as the 16-alpha-oh-a, spans at least 20 kbp, and encodes a cytochrome P-450 whose deduced amino acid sequence is 90% similar to the hydroxylase. Nucleotide sequences revealed that duplication of the two genes occurred 4-22 million years ago, and that the 5' duplication boundary is located 1336 bp upstream from the putative transcription-start site. In the flanking regions of both genes, there is a long stretch (100 bp) of CA repeats in addition to other motifs, including TATA box, glucocorticoid-response-element-core and Simian-virus-40-enhancer sequences and IgG light-chain gene promoter. We isolated many genomic DNA clones which contain exon 1 sequences, and compared their restriction maps, cross-hybridization and nucleotide sequences. The results indicate that these genomic clones represent closely related genes in the 16-alpha-oh family with a minimum of 16 members, which is further divided into classes a, b and c. 16-alpha-oh-b belong to the first and second classes, respectively. Moreover, extensive segmental gene conversion and nonreciprocal recombination were noted among the genes, particularly among those in class b. All genes in that class contain the long ATTT repeat sequences in intron 1, which may have triggered a rapid gene conversion and/or stabilize the duplicated genes. C1 NIEHS,REPROD & DEV TOXICOL LAB,PHARMACOGENET SECT,POB 12233,RES TRIANGLE PK,NC 27709. NR 31 TC 29 Z9 30 U1 0 U2 3 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0014-2956 J9 EUR J BIOCHEM JI Eur. J. Biochem. PD JAN 30 PY 1991 VL 195 IS 2 BP 477 EP 486 DI 10.1111/j.1432-1033.1991.tb15728.x PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EW783 UT WOS:A1991EW78300022 PM 1997326 ER PT J AU CONTI, MA SELLERS, JR ADELSTEIN, RS ELZINGA, M AF CONTI, MA SELLERS, JR ADELSTEIN, RS ELZINGA, M TI IDENTIFICATION OF THE SERINE RESIDUE PHOSPHORYLATED BY PROTEIN-KINASE-C IN VERTEBRATE NONMUSCLE MYOSIN HEAVY-CHAINS SO BIOCHEMISTRY LA English DT Article ID SMOOTH-MUSCLE MYOSIN; LIGHT CHAIN; CYTOPLASMIC MYOSINS; DNA-SEQUENCE; CELLS; EXPRESSION; MEROMYOSIN; PEPTIDES; ISOFORM; CLONING AB Two-dimensional mapping of the tryptic phosphopeptides generated following in vitro protein kinase C phosphorylation of the myosin heavy chain isolated from human platelets and chicken intestinal epithelial cells show a single radioactive peptide. These peptides were found to comigrate, suggesting that they were identical, and amino acid sequence analysis of the human platelet tryptic peptide yielded the sequence -Glu-Val-Ser-Ser(PO4)-Leu-Lys-. Inspection of the amino acid sequence for the chicken intestinal epithelial cell myosin heavy chain (196 kDa) derived from cDNA cloning showed that this peptide was identical with a tryptic peptide present near the carboxyl terminal of the predicted alpha-helix of the myosin rod. Although other vertebrate nonmuscle myosin heavy chains retain neighboring amino acid sequences as well as the serine residue phosphorylated by protein kinase C, this residue is notably absent in all vertebrate smooth muscle myosin heavy chains (both 204 and 200 kDa) sequenced to date. C1 NHLBI,MOLEC CARDIOL LAB,BETHESDA,MD 20892. NEW YORK STATE INST BASIC RES DEV DISABILITIES,STATEN ISL,NY 10314. OI Adelstein, Robert/0000-0002-8683-2144 FU NHLBI NIH HHS [HL 21471] NR 36 TC 59 Z9 59 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD JAN 29 PY 1991 VL 30 IS 4 BP 966 EP 970 DI 10.1021/bi00218a012 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EV178 UT WOS:A1991EV17800012 PM 1899200 ER PT J AU GIBSON, C GOLUB, E HEROLD, R RISSER, M DING, W SHIMOKAWA, H YOUNG, M TERMINE, J ROSENBLOOM, J AF GIBSON, C GOLUB, E HEROLD, R RISSER, M DING, W SHIMOKAWA, H YOUNG, M TERMINE, J ROSENBLOOM, J TI STRUCTURE AND EXPRESSION OF THE BOVINE AMELOGENIN GENE SO BIOCHEMISTRY LA English DT Article ID AMINO-ACID-SEQUENCE; MESSENGER-RNA; ELASTIN GENE; ENAMEL; PROTEINS; COMPONENTS; CDNA AB In order to define further the mechanisms responsible for tooth amelogenin heterogeneity, seven bovine amelogenin cDNAs were sequenced. On the basis of these sequences, five of the cDNAs could be grouped into one class which differed appreciably in sequence from the second group of two cDNAs. Two overlapping bovine genomic clones were then isolated and shown by sequencing to contain six exons encoding the entire consensus sequence of the class I cDNA. Southern blot analysis of DNA from male and female animals using class I or class II specific oligonucleotide probes suggested that the class I gene sequence was located on the X chromosome while the class II sequence was located on the Y chromosome. Therefore, these results also suggest that the genes on the X and Y chromosomes are both transcribed. Furthermore, the results are consistent with alternative splicing of the class I primary transcript as a potential mechanism for generating amelogenin heterogeneity. C1 UNIV PENN,SCH DENT MED,ORAL BIOL RES CTR,PHILADELPHIA,PA 19104. NIDR,BONE RES BRANCH,BETHESDA,MD 20892. UNIV PENN,SCH DENT MED,DEPT ANAT & HISTOL,PHILADELPHIA,PA 19104. FU NIDCR NIH HHS [DE-07126, DE-08063, DE-08239] NR 28 TC 80 Z9 87 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD JAN 29 PY 1991 VL 30 IS 4 BP 1075 EP 1079 DI 10.1021/bi00218a028 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EV178 UT WOS:A1991EV17800028 PM 1989679 ER PT J AU HEYES, MP SWARTZ, KJ MARKEY, SP BEAL, MF AF HEYES, MP SWARTZ, KJ MARKEY, SP BEAL, MF TI REGIONAL BRAIN AND CEREBROSPINAL-FLUID QUINOLINIC ACID CONCENTRATIONS IN HUNTINGTONS-DISEASE SO NEUROSCIENCE LETTERS LA English DT Article DE QUINOLINIC ACID; HUNTINGTONS DISEASE; EXCITOTOXIN; NEURODEGENERATION; BRAIN; CEREBROSPINAL FLUID ID RAT-BRAIN; NEUROPEPTIDE-Y; NEURONS; QUANTIFICATION; LESIONS AB Many of the characteristic neuroanatomical and neurochemical features of Huntington's disease (HD) are produced in experimental animals by an intrastriatal injection of the endogenous N-methyl-D-aspartate receptor agonist quinoline acid (QUIN). Conceivably, a chronic over-production of QUIN in brain could be involved in the pathogenesis of HD. To investigate this hypothesis, concentrations of QUIN were measured both in cerebrospinal fluid (CSF) and postmortem tissue from patients with HD and neurologically normal age-matched controls. CSF QUIN concentrations were slightly lower in patients with HD, however the changes were not significant. Mean concentrations of QUIN tended to be lower in HD putamen, dentate nucleus and several cortical regions, although significant reductions were found only in Brodmann areas 17, 20 and 28. The mechanisms responsible for these small reductions in brain QUIN concentrations remain to be determined. These results do not support the hypothesis that a chronic increase of QUIN production is responsible for neurodegeneration in HD. C1 HARVARD UNIV,SCH MED,PROGRAM NEUROSCI,BOSTON,MA 02115. HARVARD UNIV,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,NEUROL RES LABS,NEUROCHEM LAB,BOSTON,MA 02114. RP HEYES, MP (reprint author), NIMH,CLIN SCI LAB,ANALYT BIOCHEM SECT,BLDG 10,ROOM 3D40,BETHESDA,MD 20892, USA. FU NIMH NIH HHS [MHNS 31862]; PHS HHS [16367] NR 31 TC 56 Z9 57 U1 1 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD JAN 28 PY 1991 VL 122 IS 2 BP 265 EP 269 DI 10.1016/0304-3940(91)90874-S PG 5 WC Neurosciences SC Neurosciences & Neurology GA EY494 UT WOS:A1991EY49400033 PM 1827518 ER PT J AU LEW, R GRIGORIADIS, D WILSON, A BOJA, JW SIMANTOV, R KUHAR, MJ AF LEW, R GRIGORIADIS, D WILSON, A BOJA, JW SIMANTOV, R KUHAR, MJ TI DOPAMINE TRANSPORTER - DEGLYCOSYLATION WITH EXOGLYCOSIDASES AND ENDOGLYCOSIDASES SO BRAIN RESEARCH LA English DT Article DE DOPAMINE TRANSPORTER; DEGLYCOSYLATION; GLYCOSIDASE; STRIATUM ID UPTAKE SITES; COCAINE BINDING; H-3 COCAINE; GBR-12935 BINDING; STRIATAL MEMBRANES; NONHUMAN-PRIMATES; RAT STRIATUM; MOUSE-BRAIN; RECEPTORS; COMPLEX AB The dopamine transporter from rat caudate-putamen was photolabeled with [I-125]DEEP as previously described. Treatment of photolabeled membranes with neuraminidase and N-glycanase reduced the molecular weight of the [I-125]DEEP photolabeled dopamine transporter complex, whereas treatment with alpha-mannosidase had no effect. The solubilized [I-125]DEEP photolabeled dopamine transporter complex readily bound to wheat-germ agglutinin but not to concanavalin-A sepharose columns. These results suggest that the carbohydrate moiety of the dopamine transporter is N-linked and contains significant quantities of sialic acid but not high mannose residues. A DEEP binding protein was readily detectable in other brain regions including the nucleus accumbens and olfactory tubercle, but not in the prefrontal cortex, olfactory bulb or hypothalamus under similar conditions. The DEEP binding protein in the other brain regions was similar to that in the striatum. C1 NIDA,ADDICT RES CTR,NEUROSCI BRANCH,POB 5180,BALTIMORE,MD 21224. RI Wilson, Alan/A-1788-2011 NR 38 TC 53 Z9 53 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD JAN 25 PY 1991 VL 539 IS 2 BP 239 EP 246 DI 10.1016/0006-8993(91)91627-D PG 8 WC Neurosciences SC Neurosciences & Neurology GA EX406 UT WOS:A1991EX40600009 PM 2054600 ER PT J AU GAUDET, S PALKOVITS, M NAMBOODIRI, MAA AF GAUDET, S PALKOVITS, M NAMBOODIRI, MAA TI REGIONAL DISTRIBUTION OF ARYLAMINE AND ARYLALKYLAMINE N-ACETYLTRANSFERASE ACTIVITIES IN THE RAT-BRAIN SO BRAIN RESEARCH LA English DT Note DE NEUROTOXICITY; DETOXIFICATION; KYNURENINE; QUINOLINIC ACID; EXTRAPINEAL MELATONIN SYNTHESIS ID BINDING-SITES; QUANTITATION; PINEAL AB Arylamine and arylalkylamine N-acetyltransferase (NAT) activities were measured separately in different areas of the rat brain using specific substrates. Relatively high levels of arylamine NAT activity were detected in all areas examined. Arylalkylamine NAT activity in these areas ranged from 2 to 5% of the arylamine NAT activity. The possibility that arylamine NAT may be involved in the control of kynurenine metabolism in the brain is discussed. C1 GEORGETOWN UNIV,DEPT BIOL,REISS BLDG,RM 402,37TH & O ST NW,WASHINGTON,DC 20057. NIMH,CELL BIOL LAB,BETHESDA,MD 20892. RI Palkovits, Miklos/F-2707-2013 FU NIDDK NIH HHS [DK37024] NR 20 TC 14 Z9 14 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD JAN 25 PY 1991 VL 539 IS 2 BP 355 EP 357 DI 10.1016/0006-8993(91)91645-H PG 3 WC Neurosciences SC Neurosciences & Neurology GA EX406 UT WOS:A1991EX40600027 PM 2054604 ER PT J AU LIOTTA, LA STEEG, PS STETLERSTEVENSON, WG AF LIOTTA, LA STEEG, PS STETLERSTEVENSON, WG TI CANCER METASTASIS AND ANGIOGENESIS - AN IMBALANCE OF POSITIVE AND NEGATIVE REGULATION SO CELL LA English DT Review ID BASEMENT-MEMBRANE COLLAGEN; NUCLEOSIDE DIPHOSPHATE KINASE; BRONCHIAL EPITHELIAL-CELLS; HUMAN AMNIOTIC MEMBRANE; HUMAN-BREAST TUMORS; NIH 3T3 CELLS; PLASMINOGEN-ACTIVATOR; ENDOTHELIAL-CELLS; IV COLLAGENASE; TISSUE INHIBITOR RP LIOTTA, LA (reprint author), NCI,PATHOL LAB,BETHESDA,MD 20892, USA. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 113 TC 2578 Z9 2653 U1 25 U2 142 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD JAN 25 PY 1991 VL 64 IS 2 BP 327 EP 336 DI 10.1016/0092-8674(91)90642-C PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA EV336 UT WOS:A1991EV33600010 PM 1703045 ER PT J AU GILAD, GM GILAD, VH AF GILAD, GM GILAD, VH TI POLYAMINE UPTAKE, BINDING AND RELEASE IN RAT-BRAIN SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Article DE POLYAMINES; POLYAMINE BINDING; POLYAMINE UPTAKE; POLYAMINE RELEASE; BRAIN; (RAT) ID CENTRAL NERVOUS-SYSTEM; D-ASPARTATE RECEPTOR; MONKEY BRAIN; SPERMIDINE; SYNAPTOSOMES; MODULATION; PUTRESCINE; ISCHEMIA; COMPLEX; CORTEX AB The uptake, binding and release of the polyamines, spermidine and spermine, and of their diamine precursor, putrescine, were examined in synaptosomal preparations from rat hippocampus. The specific and relatively high-affinity uptake by synaptosomes was found only with putrescine (V(max) = 21.6 pmol/mg protein per h; K(m) = 28.6 nM) and not with the other polyamines. In contrast, specific binding to membranes was found for spermidine (B(max) = 28.6 pmol/mg protein; K(d) = 42.9 nM) and for spermine (B(max) = 156.3 pmol/mg protein; K(d) = 83.3 nM), but not for putrescine. High potassium concentrations (35 mM) both induced the release of accumulated polyamines from synaptosomes and inhibited their binding. Specific polyamine binding evidently occurs selectively on the inner but not on the outer surface of synaptosomal membranes. RP GILAD, GM (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032, USA. NR 29 TC 58 Z9 58 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD JAN 25 PY 1991 VL 193 IS 1 BP 41 EP 46 DI 10.1016/0014-2999(91)90198-Y PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EY215 UT WOS:A1991EY21500007 PM 2050191 ER PT J AU SHIMADA, S SPIVAK, C UHL, G AF SHIMADA, S SPIVAK, C UHL, G TI ENDOTHELIN RECEPTOR - A PROFOUNDLY DESENSITIZING RECEPTOR EXPRESSED IN XENOPUS OOCYTES SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Note DE ENDOTHELIN; SARAFOTOXIN; DESENSITIZATION; XENOPUS OOCYTES; BRAIN; (RECEPTOR) AB Xenopus oocyte expression studies can help to define the physiological properties of a receptor and can aid in receptor cloning. Expression of an endothelin receptor in oocytes injected with brain mRNA is inferred from depolarizations induced by several endothelin-related peptides. The response is abolished by intracellular EGTA injection but not in the absence of extracellular Ca2+. It is not present in non-injected oocytes, and reverses at a potential indicating that it is mediated by an increased Cl-1 conductance. Responses display striking, long-lasting desensitization. The expressed endothelin receptor thus displays properties characteristic of several receptors coupled to changes in phosphoinositide turnover, several of which have been successfully cloned using this response as a reporter. C1 NIDA,ARC,MOLEC NEUROBIOL LAB,BOX 5180,BALTIMORE,MD 21224. NIDA,ARC,NEUROPHARMACOL LAB,BALTIMORE,MD 21224. JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205. NR 9 TC 8 Z9 8 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD JAN 25 PY 1991 VL 193 IS 1 BP 123 EP 125 DI 10.1016/0014-2999(91)90211-8 PG 3 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EY215 UT WOS:A1991EY21500020 PM 1710987 ER PT J AU MOSS, B AHN, BY AMEGADZIE, B GERSHON, PD KECK, JG AF MOSS, B AHN, BY AMEGADZIE, B GERSHON, PD KECK, JG TI CYTOPLASMIC TRANSCRIPTION SYSTEM ENCODED BY VACCINIA VIRUS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Review ID DEPENDENT RNA-POLYMERASE; TEMPERATURE-SENSITIVE MUTANTS; NUCLEOSIDE TRIPHOSPHATE PHOSPHOHYDROLASES; ENZYME-GUANYLATE INTERMEDIATE; LATE MESSENGER-RNAS; EARLY GENES; PHENOTYPIC CHARACTERIZATION; DNA TOPOISOMERASE; CAPPING ENZYME; POLY(A) HEAD RP MOSS, B (reprint author), NIAID,VIRAL DIS LAB,BETHESDA,MD 20892, USA. NR 87 TC 101 Z9 103 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 25 PY 1991 VL 266 IS 3 BP 1355 EP 1358 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU497 UT WOS:A1991EU49700001 PM 1988424 ER PT J AU KIM, UH FINK, D KIM, HS PARK, DJ CONTRERAS, ML GUROFF, G RHEE, SG AF KIM, UH FINK, D KIM, HS PARK, DJ CONTRERAS, ML GUROFF, G RHEE, SG TI NERVE GROWTH-FACTOR STIMULATES PHOSPHORYLATION OF PHOSPHOLIPASE C-GAMMA IN PC12 CELLS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Note ID PROTEIN KINASE-C; TYROSINE PHOSPHORYLATION; PHEOCHROMOCYTOMA CELLS; SIGNAL TRANSDUCTION; RECEPTOR; ACETYLCHOLINE; ACTIVATION; MECHANISM; CALCIUM; GAMMA AB PC12 cells contain at least three immunologically distinct phospholipase C (PLC) isozymes, PLC-beta, PLC-gamma, and PLC-delta. Treatment of PC12 cells with nerve growth factor (NGF) leads to an increase in the phosphorylation of PLC-gamma, but not of PLC-beta or PLC-delta. This increase can be seen in as little as 1 minute. The increased phosphorylation occurs on both serine and tyrosine residues, with the major increase being in the former. This result suggests the possibility that the NGF-dependent increase in phosphoinositide hydrolysis in PC12 cells is due to selective phosphorylation of PLC-gamma by serine and tyrosine protein kinases associated with the NGF receptor. C1 NHLBI,ZENTRUM BIOCHEM,SIGNAL TRANSDUCT SECT,BETHESDA,MD 20892. NICHHD,GROWTH FACTORS SECT,BETHESDA,MD 20892. RI Park, Do-Joon/J-2736-2012 NR 40 TC 162 Z9 163 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 25 PY 1991 VL 266 IS 3 BP 1359 EP 1362 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU497 UT WOS:A1991EU49700002 PM 1703147 ER PT J AU HOLLINGSWORTH, HC NOSSAL, NG AF HOLLINGSWORTH, HC NOSSAL, NG TI BACTERIOPHAGE-T4 ENCODES AN RNASE-H WHICH REMOVES RNA PRIMERS MADE BY THE T4 DNA-REPLICATION SYSTEM INVITRO SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MURINE LEUKEMIA-VIRUS; ESCHERICHIA-COLI; RIBONUCLEASE-H; PRIMASE-HELICASE; REVERSE-TRANSCRIPTASE; NUCLEOTIDE-SEQUENCE; DEFECTIVE-MUTANTS; STRONG PROMOTERS; POLYMERASE-I; GENE AB RNase H activity increases markedly after bacteriophage T4 infection of Escherichia coli MIC2003, an RNase H-deficient host. We have extensively purified the RNase H from these T4-infected cells and have shown that the RNase H activity copurifies with a 5' to 3' DNA exonulcease activity. The N-terminal sequence of a 35-kDa protein copurifying with the RNase H activity matches the terminus of the predicted product of an open reading frame (designated ORF A or 33.2) upstream of T4 gene 33, identified previously by Hahn and co-workers (Hahn, S., Kruse, U., and Ruger, W. (1986) Nucleic Acids Res. 14, 9311-9327). Plasmids containing ORF A under the control of the T7 promoter express RNase H and 5' to 3' DNA exonuclease activities as well as a protein that comigrates on sodium dodecyl sulfate-polyacrylamide gels with the 35-kDa protein present in the RNase H purified from T4-infected cells. T4 RNase H removes the pentamer RNA primers from DNA chains initiated by the T4 primase-helicase (gene products 61 and 41). Addition of T4 RNase H and T4 DNA ligase leads to extensive joining of discontinuous lagging strand fragments in the T4 DNA replication system in vitro. C1 NIDDKD,BIOCHEM PHARMACOL LAB,NUCL ACID BIOCHEM SECT,BETHESDA,MD 20892. NR 63 TC 51 Z9 52 U1 1 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 25 PY 1991 VL 266 IS 3 BP 1888 EP 1897 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU497 UT WOS:A1991EU49700079 PM 1703156 ER PT J AU KITANI, S BERENSTEIN, E MERGENHAGEN, S TEMPST, P SIRAGANIAN, RP AF KITANI, S BERENSTEIN, E MERGENHAGEN, S TEMPST, P SIRAGANIAN, RP TI A CELL-SURFACE GLYCOPROTEIN OF RAT BASOPHILIC LEUKEMIA-CELLS CLOSE TO THE HIGH-AFFINITY IGE RECEPTOR (FC-EPSILON-RI) - SIMILARITY TO HUMAN-MELANOMA DIFFERENTIATION ANTIGEN ME491 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HISTAMINE-RELEASE; MONOCLONAL-ANTIBODY; IMMUNOGLOBULIN-E; MAST-CELLS; TRANSFECTED CELLS; BINDING; VARIANTS; EXPRESSION; INHIBITION; HYBRIDOMAS AB A monoclonal antibody (mAb), AD1, was isolated that recognized a cell surface protein on rat basophilic leukemia cells (RBL-2H3). At high concentration, this antibody inhibited IgE-mediated but not calcium ion-ophore-induced histamine release (49% inhibition at 100-mu-g/ml). The mAb AD1 did not inhibit the binding of IgE or of several antibodies directed to the high affinity IgE receptor (Fc-epsilon-RI). Likewise, IgE did not inhibit mAb AD1 binding. However, several anti-Fc-epsilon-RI antibodies did inhibit mAb AD1 binding as intact molecules but not as Fab fragments. Therefore, the sites on the cell surface to which mAb AD1 binds are close to Fc-epsilon-RI. The mAB AD1 immunoprecipitated a broad, 50-60-kDa band from I-125-surface-labeled RBL-2H3 cells that upon peptide N-glycosidase F treatment was transformed into a sharp 27-kDa band. A similar 27-kDa protein was immunoprecipitated from surface-radiolabeled cells after culture with tunicamycin. Thus, the protein recognized by mAb AD1 is highly glycosylated with predominatly N-linked oligosaccharides. The N-terminal sequence of 43 amino acids was found to be different from any subunit of Fc-epsilon-RI but nearly identical to that of the human melanoma-associated antigen ME491. Therefore, mAb AD1 binds to a surface glycoprotein on RBL-2H3 cells sterically close to the Fc-epsilon-RI but distinct from the recognized subunits of the receptor. C1 HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02115. RP KITANI, S (reprint author), NIDR,IMMUNOL LAB,CLIN IMMUNOL SECT,BETHESDA,MD 20892, USA. RI Wan, Daniel/F-4689-2010 NR 32 TC 75 Z9 76 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 25 PY 1991 VL 266 IS 3 BP 1903 EP 1909 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU497 UT WOS:A1991EU49700081 PM 1703158 ER PT J AU PINES, E HUPPERT, D AGMON, N AF PINES, E HUPPERT, D AGMON, N TI SALT EFFECTS ON STEADY-STATE QUANTUM YIELDS OF ULTRAFAST, DIFFUSION-INFLUENCED, REVERSIBLE PHOTOACID DISSOCIATION REACTIONS SO JOURNAL OF PHYSICAL CHEMISTRY LA English DT Article ID PROTON-TRANSFER REACTIONS; GEMINATE RECOMBINATION; PH JUMP; ELECTRON-TRANSFER; AQUEOUS-SOLUTIONS; IONIC-SOLUTIONS; EQUILIBRIUM; KINETICS; MODEL; CAGE AB The steady-state fluorescence quantum yields of the protonated and deprotonated forms of 8-hydroxyprene-1,3,6-trisulfonate are determined in three solvents as a function of NaNO3 concentration. The measured quantum yields, and therefore also the overall dissociation rate coefficients, decrease with increasing salt concentration, in contrast to Bronsted's classical model for primary salt effects. This is a consequence of the reversibility and the nearly diffusion-controlled recombination rate of the proton in the reaction studied. A quantitative analysis is based on newly derived results for reversible diffusion-influenced reactions. Comparison with experiment is made for two screened potentials. Numerical integration with the Debye-Huckel potential indicates that it overestimates the salt effect. A second model (the "naive approximation") is based on a simple screened potential form which analytical expressions for reaction yields and rates are derived. It shows better agreement with experiment in the range 20-200 mM salt. C1 TEL AVIV UNIV,RAYMOND & BEVERLY SACKLER FAC EXACT SCI,IL-69978 TEL AVIV,ISRAEL. NIDDKD,DIV COMP RES & TECHNOL,PHYS SCI LAB,BETHESDA,MD 20892. NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. RI Agmon, Noam/B-2611-2009 OI Agmon, Noam/0000-0003-4339-8664 NR 65 TC 40 Z9 40 U1 0 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-3654 J9 J PHYS CHEM-US JI J. Phys. Chem. PD JAN 24 PY 1991 VL 95 IS 2 BP 666 EP 674 DI 10.1021/j100155a032 PG 9 WC Chemistry, Physical SC Chemistry GA EU486 UT WOS:A1991EU48600032 ER PT J AU MANASCO, PK GIRTON, ME DIGGS, RL DOPPMAN, JL FEUILLAN, PP BARNES, KM CUTLER, GB LORIAUX, DL ALBERTSON, BD AF MANASCO, PK GIRTON, ME DIGGS, RL DOPPMAN, JL FEUILLAN, PP BARNES, KM CUTLER, GB LORIAUX, DL ALBERTSON, BD TI A NOVEL TESTIS-STIMULATING FACTOR IN FAMILIAL MALE PRECOCIOUS PUBERTY SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID LUTEINIZING-HORMONE; SEXUAL PRECOCITY; LEYDIG; RADIOIMMUNOASSAY; GONADOTROPIN; TESTOSTERONE; THERAPY AB Background. Familial male precocious puberty is a gonadotropin-independent form of precocious puberty that occurs only in males. The cause of the disorder is unknown. To examine the hypothesis that the plasma of boys with familial male precocious puberty contains a novel stimulator of testicular testosterone production, we developed a bioassay using adult male cynomolgus monkeys. Methods. We collected plasma from 12 boys with familial male precocious puberty, 7 normal prepubertal boys of similar ages and with similar plasma gonadotropin levels, and 1 boy with hypogonadotropic hypogonadism and infused it into the testicular artery of adult male cynomolgus monkeys that had been pretreated with gonadotropin-releasing-hormone antaganist to inhibit the endogenous secretion of gonadotropins. Testicular venous effluent was collected at 15-minute intervals for 3 to 5 hours for the measurement of testosterone. Results. The mean (+/- SE) peak testosterone response, as compared with base line, was significantly greater in the monkeys infused with plasma from the 12 boys with familial male precocious puberty than in the monkeys infused with plasma from the 7 normal prepubertal boys and the boy with hypogonadotropic hypogonadism (385 +/- 51 vs. 184 +/- 25 percent, P < 0.005) in the three-hour studies. Plasma from 92 percent of the boys with familial male precocious puberty and 12.5 percent of the normal prepubertal boys stimulated a response greater than 195 percent of base-line values. In the animals studied for five hours after receiving a second dose of antagonist, the mean peak testosterone response, as compared with base line, was significantly greater in the monkeys infused with plasma from three boys with familial male precocious puberty than in the monkeys infused with plasma from three normal prepubertal boys (363 +/- 81 vs. 115 +/- 6 percent, P < 0.01). The mean area under the testosterone-response curve wa significantly larger in the monkeys infused with plasma from the boys with familial male precocious puberty in the five-hour studies (154 +/- 34 vs. -58 +/- 10 percent, P < 0.005), but not in the three-hour studies. Conclusions. These findings support the presence of a circulating testis-stimulating factor in the plasma of boys with familial male precocious puberty. The production of such a factor would explain the biologic nature of the disorder. C1 NICHHD,DEV ENDOCRINOL BRANCH,BLDG 10,RM 10N262,BETHESDA,MD 20892. NIH,CTR CLIN,DEPT RADIOL,BETHESDA,MD 20892. GEORGETOWN UNIV HOSP,DEPT RADIOL,WASHINGTON,DC 20007. NR 14 TC 19 Z9 19 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 24 PY 1991 VL 324 IS 4 BP 227 EP 231 DI 10.1056/NEJM199101243240404 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA EU022 UT WOS:A1991EU02200004 PM 1898671 ER PT J AU POLDER, JA BELL, DM MARTONE, WJ MARTIN, LS CURRAN, JW BENINGER, P HENDERSON, DK AF POLDER, JA BELL, DM MARTONE, WJ MARTIN, LS CURRAN, JW BENINGER, P HENDERSON, DK TI ZIDOVUDINE AFTER OCCUPATIONAL EXPOSURE TO THE HUMAN-IMMUNODEFICIENCY-VIRUS - REPLY SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 US FDA,ROCKVILLE,MD 20857. NIH,BETHESDA,MD 20892. RP POLDER, JA (reprint author), CTR DIS CONTROL,ATLANTA,GA 30333, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 24 PY 1991 VL 324 IS 4 BP 266 EP 267 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA EU022 UT WOS:A1991EU02200014 ER PT J AU DILSIZIAN, V BONOW, RO AF DILSIZIAN, V BONOW, RO TI REINJECTION OF THALLIUM AFTER STRESS REDISTRIBUTION IMAGING - REPLY SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter RP DILSIZIAN, V (reprint author), NHLBI,BETHESDA,MD 20892, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 24 PY 1991 VL 324 IS 4 BP 270 EP 270 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EU022 UT WOS:A1991EU02200022 ER PT J AU HOWARD, FB CHEN, C ROSS, PD MILES, HT AF HOWARD, FB CHEN, C ROSS, PD MILES, HT TI HAIRPIN FORMATION IN THE SELF-COMPLEMENTARY DODECAMER D-GGTACGCGTACC AND DERIVATIVES CONTAINING GA-MISPAIRS AND IA-MISPAIRS SO BIOCHEMISTRY LA English DT Article ID HELIX FORMATION; DNA; STABILITY; ACIDS AB The dodecamer d-GGTACGCGTACC and four derivatives with GA and IA mispairs in the 6,7 and 5,8 positions have been examined in dilute solution and 0.01-0.1 M sodium chloride. Concentration dependence of T(m), gel electrophoresis, and equilibrium centrifugation indicate that these self-complementary oligomers can forms hairpins under the present conditions. Thermal transitions measured in the ultraviolet primarily represent melting of hairpin to coil [cf. Scheffler et al. (1968, 1970)]. The T(m) values show little or no depression for 6,7 substitution but rather large depression for 5,8 replacement. We interpret the results to indicate that the 6,7 sequences have two-base loops and five base pair stems and that the 5,8 sequences have four-base loops and four base pair stems. A concurrent theoretical modeling study [Raghunathan et al. (1991) Biochemistry (following paper in this tissue)] provides support for this interpretation. C1 NIDDKD,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 19 TC 25 Z9 25 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD JAN 22 PY 1991 VL 30 IS 3 BP 779 EP 782 DI 10.1021/bi00217a030 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU205 UT WOS:A1991EU20500030 PM 1988065 ER PT J AU RAGHUNATHAN, G JERNIGAN, RL MILES, HT SASISEKHARAN, V AF RAGHUNATHAN, G JERNIGAN, RL MILES, HT SASISEKHARAN, V TI CONFORMATIONAL FEASIBILITY OF A HAIRPIN WITH 2 PURINES IN THE LOOP - 5'-D-GGTACIAGTACC-3' SO BIOCHEMISTRY LA English DT Article ID NUCLEIC-ACIDS; DNA HAIRPIN; BACKBONE STRUCTURE; POLYNUCLEOTIDES; STABILITY; ENERGY; CONSTITUENTS; DYNAMICS; FRAGMENT; DUPLEX AB Structural feasibility and conformational requirements for the sequence 5'-d-GGTACIAGTACC-3' to adopt a hairpin loop with 16 and A7 in the loop are studied. It is shown that a hairpin loop containing only two nucleotides can readily be formed without any unusual torsional angles. Stacking is continued on the 5'-side of the loop, with the 16 stacked upon C5. The base A7, on the 3'-side of the loop, can either be partially stacked with 16 or stick outside without stacking. Loop closure can be achieved for both syn and anti conformations of the glycosidic torsions for G8 while maintaining the normal Watson-Crick base pairing with the opposite C5. All torsional angles in the stem fall within the standard B-family of DNA helical structures. The phosphodiesters of the loop have trans, trans conformations. Loop formation might require the torsion about the C4'-C5' bond of G8 to be trans as opposed to the gauche+ observed in B-DNA. These results are discussed in relation to melting temperature studies [Howard et al. (1991) Biochemistry (preceding paper in this issue)] that suggest the formation of very stable hairpin structures for this sequence. C1 NIDDKD,MOLEC BIOL LAB,BLDG 2,ROOM 201,BETHESDA,MD 20892. NCI,MATH BIOL LAB,BETHESDA,MD 20892. RI Jernigan, Robert/A-5421-2012 NR 38 TC 22 Z9 22 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD JAN 22 PY 1991 VL 30 IS 3 BP 782 EP 788 DI 10.1021/bi00217a031 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU205 UT WOS:A1991EU20500031 PM 1988066 ER PT J AU KANTOR, RRS GIARDINA, SL BARTOLAZZI, A TOWNSEND, AJ MYERS, CE COWAN, KH LONGO, DL NATALI, PG AF KANTOR, RRS GIARDINA, SL BARTOLAZZI, A TOWNSEND, AJ MYERS, CE COWAN, KH LONGO, DL NATALI, PG TI MONOCLONAL-ANTIBODIES TO GLUTATHIONE-S-TRANSFERASE PI-IMMUNOHISTOCHEMICAL ANALYSIS OF HUMAN TISSUES AND CANCERS SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID RAT CHEMICAL HEPATOCARCINOGENESIS; PLACENTAL FORM; CELL-LINES; ELECTROPHORETIC TRANSFER; NITROCELLULOSE SHEETS; POLYACRYLAMIDE GELS; PAPILLOMAVIRUS DNA; MELANOMA-CELLS; GST-PI; PURIFICATION AB Mouse monoclonal antibodies (MAb) have been generated against the anionic isozyme of human glutathione S-transferase (GST-pi). MAb AGST 1 can inhibit 50-70% of GST-pi enzymatic activity and reacts with a 3-dimensional epitope which includes a putative glutathione binding site on GST-pi. A sandwich enzyme-immunoassay established using MAb AGST 1 and a polyclonal antibody displayed a sensitivity of 0.5 ng/ml. Immunohistochemical analysis of human tissues demonstrated marked increases in GST-pi levels in cancers of the brain, cervix, endometrium, colon, rectum and testis and in fibro- and chondrosarcomas. C1 REGINA ELENA INST CANC RES,DEPT IMMUNOL,ROME,ITALY. NCI,DIV CANC TREATMENT,MED BRANCH,BETHESDA,MD 20892. NCI,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701. RP KANTOR, RRS (reprint author), NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,DYNCORP,FREDERICK,MD 21701, USA. FU NCI NIH HHS [N01-CO-74102] NR 52 TC 58 Z9 60 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD JAN 21 PY 1991 VL 47 IS 2 BP 193 EP 201 DI 10.1002/ijc.2910470206 PG 9 WC Oncology SC Oncology GA EV221 UT WOS:A1991EV22100005 PM 1703126 ER PT J AU KORN, EL BAUMRIND, S AF KORN, EL BAUMRIND, S TI RANDOMIZED CLINICAL-TRIALS WITH CLINICIAN-PREFERRED TREATMENT SO LANCET LA English DT Article ID CANCER AB The standard design for randomised clinical trials may be inappropriate when the clinician believes that one of the treatments being tested is superior for the patient, or when the clinician has a preference for one of the treatments. For such instances the suggestion is that the patient is randomly allocated to treatment only when there is clinical disagreement about treatment of choice for that patient, and then the patient is assigned to a clinician who had thought that the regimen allocated is the one most appropriate for that patient. C1 UNIV CALIF SAN FRANCISCO,SCH DENT,DEPT GROWTH & DEV,SAN FRANCISCO,CA 94143. RP KORN, EL (reprint author), NCI,BIOMETR RES BRANCH,EXECUT PLAZA N,ROOM 739,BETHESDA,MD 20892, USA. NR 18 TC 36 Z9 36 U1 1 U2 2 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD JAN 19 PY 1991 VL 337 IS 8734 BP 149 EP 152 DI 10.1016/0140-6736(91)90809-4 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA ET679 UT WOS:A1991ET67900012 PM 1670796 ER PT J AU MANNS, A MURPHY, EL WILKS, RJ HAYNES, G FIGUEROA, JP HANCHARD, B PALKER, TJ BLATTNER, WA AF MANNS, A MURPHY, EL WILKS, RJ HAYNES, G FIGUEROA, JP HANCHARD, B PALKER, TJ BLATTNER, WA TI EARLY ANTIBODY PROFILE DURING HTLV-I SEROCONVERSION SO LANCET LA English DT Letter C1 SAN FRANCISCO GEN HOSP,SAN FRANCISCO,CA 94110. UNIV W INDIES,KINGSTON 7,JAMAICA. NATL TRANSFUS SERV,KINGSTON,JAMAICA. MINIST HLTH,KINGSTON,JAMAICA. DUKE UNIV,DURHAM,NC 27706. RP MANNS, A (reprint author), NCI,BETHESDA,MD 20892, USA. NR 5 TC 6 Z9 6 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD JAN 19 PY 1991 VL 337 IS 8734 BP 181 EP 182 DI 10.1016/0140-6736(91)90852-G PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA ET679 UT WOS:A1991ET67900056 PM 1670825 ER PT J AU ITO, Y AF ITO, Y TI RECENT ADVANCES IN COUNTERCURRENT CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY LA English DT Review ID COIL PLANET CENTRIFUGE; LIQUID PARTITION CHROMATOGRAPHY; MULTILAYER COILS; ROTATING SEALS; PREPARATIVE SEPARATIONS; ELUTION CENTRIFUGE; STATIONARY PHASE; CELL-SEPARATION; SOLID SUPPORT; ROTARY SEALS AB During the past several years, counter-current chromatography (CCC) technology has been advanced to cover a broad spectrum of applications, from large-scale preparative to analytical-scale separations. These advances include liquid-liquid dual CCC, foam CCC and partition of macromolecules with aqueous-aqueous polymer systems. For these developments the synchronous coil planet centrifuge scheme has been used, which relies on a relatively simple mechanical design. Future developments in CCC may be focused on the improvement of the more intricate non-synchronous coil planet centrifuge scheme which has a greater potential for the separation of biopolymers and cell particles. RP ITO, Y (reprint author), NHLBI,BIOPHYS CHEM LAB,BLDG 10,ROOM 7N-322,BETHESDA,MD 20892, USA. NR 56 TC 79 Z9 84 U1 3 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 3 EP 25 DI 10.1016/S0021-9673(01)91617-6 PG 23 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400002 PM 2050794 ER PT J AU SCHAUFELBERGER, DE AF SCHAUFELBERGER, DE TI APPLICATIONS OF ANALYTICAL HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY IN NATURAL-PRODUCTS CHEMISTRY SO JOURNAL OF CHROMATOGRAPHY LA English DT Review ID COIL PLANET CENTRIFUGE; PARTITION CHROMATOGRAPHY; SEPARATION; SPECTROMETRY; UV AB Applications of analytical high-speed counter-current chromatography in natural products chemistry are reviewed and the potential of the method is discussed. C1 NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74102] NR 42 TC 13 Z9 15 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 45 EP 57 DI 10.1016/S0021-9673(01)91620-6 PG 13 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400005 PM 1646832 ER PT J AU ITO, Y KITAZUME, E BHATNAGAR, M TRIMBLE, FD AF ITO, Y KITAZUME, E BHATNAGAR, M TRIMBLE, FD TI CROSS-AXIS SYNCHRONOUS FLOW-THROUGH COIL PLANET CENTRIFUGE (TYPE-XLL) .1. DESIGN OF THE APPARATUS AND STUDIES ON RETENTION OF STATIONARY PHASE SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID PREPARATIVE COUNTERCURRENT CHROMATOGRAPHY; PARTITION EFFICIENCY; MULTILAYER COILS; ROTARY SEALS; ACCELERATION; SEPARATIONS AB The fourth prototype holds a pair of column holders in the lateral position at 15 cm from the center of the rotary shaft horizontally mounted on the rotary frame at 7.6 cm from the central axis of the apparatus. Using short coils of 2.6 mm I.D. PTFE (polytetrafluoroethylene) tubing with 7.6 cm and 24 cm helical diameters, retention of the stationary phase was measured in ten pairs of two-phase solvent systems under various experimental conditions. Satisfactory retention was obtained by choosing proper combinations of three factors, i.e., the direction of planetary motion, head-tail elution mode, and inward-outward elution mode. The polar butanol solvent systems showed excellent retention from 65 to 80% in the 7.6 cm helical diameter left-handed coil. C1 NIH,DIV RES SERV,BIOMED ENGN & INSTRUMENTAT BRANCH,BETHESDA,MD 20892. RP ITO, Y (reprint author), NHLBI,BIOPHYS CHEM LAB,BLDG 10,ROOM 7N-322,BETHESDA,MD 20892, USA. NR 10 TC 39 Z9 41 U1 3 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 59 EP 66 DI 10.1016/S0021-9673(01)91621-8 PG 8 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400006 PM 2050795 ER PT J AU ITO, Y AF ITO, Y TI CROSS-AXIS SYNCHRONOUS FLOW-THROUGH COIL PLANET CENTRIFUGE (TYPE-XLL) .2. SPECULATION ON THE HYDRODYNAMIC MECHANISM IN STATIONARY PHASE RETENTION SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID SPEED COUNTERCURRENT CHROMATOGRAPHY; SOLVENT SYSTEMS; BEHAVIOR; APPARATUS AB The hydrodynamic mechanism involved in the retention of stationary phase in the present x-axis coil planet centrifuge system is discussed. A statistical treatment of the retention data disclosed important clues such as the effect of the inward-outward elution mode, the close correlation between the planetary motion and the head-tail elution mode, and the superior retention capacity of the left-handed coils. The combined effects of the radial and lateral centrifugal force field derived from the mathematical analysis of acceleration acting on the coil provide an explanation for the phenomena. RP ITO, Y (reprint author), NHLBI,BIOPHYS CHEM LAB,BLDG 10,ROOM 7N-322,BETHESDA,MD 20892, USA. NR 8 TC 10 Z9 10 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 67 EP 80 DI 10.1016/S0021-9673(01)91622-X PG 14 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400007 PM 2050796 ER PT J AU ITO, Y KITAZUME, E SLEMP, JL AF ITO, Y KITAZUME, E SLEMP, JL TI IMPROVED HIGH-SPEED COUNTERCURRENT CHROMATOGRAPH WITH 3 MULTILAYER COILS CONNECTED IN SERIES .4. EVALUATION OF PREPARATIVE CAPABILITY WITH LARGE MULTILAYER COILS SO JOURNAL OF CHROMATOGRAPHY LA English DT Note C1 NIH,BIOMED ENGN & INSTRUMENTAT BRANCH,BETHESDA,MD 20892. RP ITO, Y (reprint author), NHLBI,BIOPHYS CHEM LAB,BLDG 10,ROOM 7N-322,BETHESDA,MD 20892, USA. NR 3 TC 14 Z9 14 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 81 EP 85 DI 10.1016/S0021-9673(01)91623-1 PG 5 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400008 PM 2050797 ER PT J AU SCHAUFELBERGER, DE MCCLOUD, TG BEUTLER, JA AF SCHAUFELBERGER, DE MCCLOUD, TG BEUTLER, JA TI LASER-LIGHT-SCATTERING DETECTION FOR HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY LA English DT Note ID LIQUID-CHROMATOGRAPHY C1 NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,FREDERICK,MD 21702. RP SCHAUFELBERGER, DE (reprint author), NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,CHEM SYNTH & ANAL LAB,POB B,FREDERICK,MD 21702, USA. RI Beutler, John/B-1141-2009 OI Beutler, John/0000-0002-4646-1924 FU NCI NIH HHS [N01-CO-74102] NR 9 TC 16 Z9 17 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 87 EP 90 DI 10.1016/S0021-9673(01)91624-3 PG 4 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400009 PM 2050798 ER PT J AU OKA, F OKA, H ITO, Y AF OKA, F OKA, H ITO, Y TI SYSTEMATIC SEARCH FOR SUITABLE 2-PHASE SOLVENT SYSTEMS FOR HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID COIL PLANET CENTRIFUGE AB We have introduced two series of two-phase solvent systems which facilitate the systematic search for the solvent systems suitable for high-speed counter-current chromatography. The n-hexane-ethyl acetate-n-butanol-methanol-water systems provide a broad range of hydrophobicity, while the chloroform-methanol-water systems are extremely useful for separations of various natural products with moderate hydrophobicity. The practical use of these solvent series was demonstrated with several test samples which include dinitrophenyl amino acids, S-triazine herbicides, indole auxins, and non-ionic organic solvents. C1 NHLBI,TECH DEV LAB,BETHESDA,MD 20892. NR 8 TC 117 Z9 128 U1 5 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 99 EP 108 DI 10.1016/S0021-9673(01)91626-7 PG 10 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400011 PM 2050799 ER PT J AU TSAI, RS ELTAYAR, N TESTA, B ITO, Y AF TSAI, RS ELTAYAR, N TESTA, B ITO, Y TI TOROIDAL COIL CENTRIFUGAL PARTITION CHROMATOGRAPHY, A METHOD FOR MEASURING PARTITION-COEFFICIENTS SO JOURNAL OF CHROMATOGRAPHY LA English DT Note ID HYDROPHOBIC PARAMETERS; LIQUID-CHROMATOGRAPHY; OCTANOL; SYSTEMS; WATER C1 UNIV LAUSANNE,SCH PHARM,PL CHATEAU,CH-1005 LAUSANNE,SWITZERLAND. NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. NR 14 TC 8 Z9 8 U1 0 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 119 EP 123 DI 10.1016/S0021-9673(01)91628-0 PG 5 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400013 PM 2050785 ER PT J AU KITAZUME, E BHATNAGAR, M ITO, Y AF KITAZUME, E BHATNAGAR, M ITO, Y TI SEPARATION OF RARE-EARTH ELEMENTS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID CENTRIFUGAL PARTITION CHROMATOGRAPHY AB Besides being widely used in electronic and glass industries, rare earth elements have recently been found to have important biological effects including the ability to stabilize and enhance interferon activity [J. J. Sedmak and S. E. Grossberg, J. Gen. Virol., 52 (1981) 195]. In this paper, the rare earth elements have been separated using a high-speed counter-current chromatography (HSCCC) centrifuge equipped with three multilayer coils connected in series. Two-phase solvent systems were composed of n-heptane containing di(2-ethylhexyl)phosphoric acid (stationary phase) and dilute hydrochloric acid (mobile phase) where the partition coefficient of each can be optimized by selecting the proper hydrochloric acid concentration. The mobile phase was eluted through the column at a flow-rate of 5 ml/min, while the apparatus was rotated at 900 rpm. Continuous detection of the rare earth elements was effected by means of a post-column reaction with arsenazo III and the elution curve was obtained by on-line monitoring at 650 nm. Excellent isocratic separations of closely related rare earth elements were achieved at high partition efficiencies up to several thousand theoretical plates. Versatility of the present method was demonstrated in an exponential gradient elution of hydrochloric acid concentration where fourteen rare earth elements were all resolved in about 4.5 h. C1 NHLBI,BIOPHYS CHEM LAB,BLDG 10,ROOM 7N-322,BETHESDA,MD 20892. NR 14 TC 33 Z9 33 U1 4 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 133 EP 140 DI 10.1016/S0021-9673(01)91630-9 PG 8 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400015 PM 2050786 ER PT J AU KNIGHT, M GLUCH, S TAKAHASHI, K DANG, TT KAHN, RA AF KNIGHT, M GLUCH, S TAKAHASHI, K DANG, TT KAHN, RA TI PURIFICATION OF A SYNTHETIC MYRISTYLATED PEPTIDE BY COUNTERCURRENT CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID BINDING AB A preparative purification of myristyl-Gly-Asn-Ile-Phe-Ala-Asn-Leu-Phe-Lys-Gly-Leu-Phe-Gly-Lys-Lys-Glu-NH2 was accomplished using the multi-coil counter-current chromatograph. A partition coefficient was determined in the n-butanol-acetic acid-water (4:1:5) system. Chromatographic runs were made in this system and one modified with ethyl acetate. The peptide material showed anomalous elution behavior due to its surfactant properties. It was found that by loading the sample exclusively in the stationary phase, satisfactory retention of the compound occurred. Finally, conditions utilizing the upper phase as the mobile phase successfully separated the impurities. C1 NCI,BIOL CHEM LAB,BETHESDA,MD 20892. RP KNIGHT, M (reprint author), PEPTIDE TECHNOL CORP,125 MICHIGAN AVE NE,WASHINGTON,DC 20017, USA. OI Knight, Martha/0000-0003-4863-8858 NR 10 TC 6 Z9 6 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 141 EP 147 DI 10.1016/S0021-9673(01)91631-0 PG 7 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400016 PM 2050787 ER PT J AU OKA, H IKAI, Y KAWAMURA, N HAYAKAWA, J YAMADA, MS HARADA, KI MURATA, H SUZUKI, M NAKAZAWA, H SUZUKI, S SAKITA, T FUJITA, M MAEDA, Y ITO, Y AF OKA, H IKAI, Y KAWAMURA, N HAYAKAWA, J YAMADA, MS HARADA, KI MURATA, H SUZUKI, M NAKAZAWA, H SUZUKI, S SAKITA, T FUJITA, M MAEDA, Y ITO, Y TI PURIFICATION OF FOOD COLOR RED-106 (ACID RED) USING HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; UNSULFONATED AROMATIC-AMINES; COIL PLANET CENTRIFUGE; C YELLOW NO-6; COAL-TAR DYES; SUBSIDIARY COLORS; DIAZOTIZATION; SEPARATION AB High-speed counter-current chromatography (HSCCC) has been successfully applied to the separation of the components of Food Color Red No. 106 (R-106). The separation was performed using 25 mg of the sample with a two-phase solvent system composed of n-butanol and 0.01 M trifluoroacetic acid (1:1, v/v). Analyses by thin-layer chromatography, high-performance liquid chromatography and fast atom bombardment mass spectrometry confirmed that HSCCC was effective in the purification of the components of R-106. The separation gave 21 mg of a 99.9% pure main component (Acid Red) and 0.9 mg of 98.0% pure subsidiary dye which is probably a des-ethyl derivative. C1 MEIJO UNIV,FAC PHARM,TEMPA KU,NAGOYA,AICHI 468,JAPAN. NATL INST PUBL HEALTH,MINATO KU,TOKYO 108,JAPAN. SHIZUOKA PREFECTURAL INST PUBL HLTH & ENVIRONM SCI,SHIZUOKA CITY 420,JAPAN. NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP OKA, H (reprint author), AICHI PREFECTURAL INST PUBL HLTH,KITA KU,TSUJI,NAGOYA 462,JAPAN. NR 19 TC 11 Z9 11 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 149 EP 156 DI 10.1016/S0021-9673(01)91632-2 PG 8 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400017 PM 2050788 ER PT J AU WEISZ, A LANGOWSKI, AJ MEYERS, MB THIEKEN, MA ITO, Y AF WEISZ, A LANGOWSKI, AJ MEYERS, MB THIEKEN, MA ITO, Y TI PREPARATIVE PURIFICATION OF TETRABROMOTETRACHLOROFLUORESCEIN AND PHLOXINE-B BY CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID PERFORMANCE; SEPARATION; DYES AB A centrifugal counter-current chromatographic method for preparative purification of commercial tetrabromotetrachlorofluorescein and Phloxine B (D&C Red Nos. 27 and 28, respectively) was developed. Ethyl acetate-n-butanol-0.01 M ammonium acetate (1:1:2) was used as the two-phase solvent system. Each purification trial involved 50 mg of sample and yielded 22 mg (+/- 2 mg) of pure dye. The purity of the product was measured by high-performance liquid and thin-layer chromatography and was found to be 99.9%. The partition coefficients of these compounds were found to be highly concentration-dependent in the two-phase solvent system used. If this problem can be circumvented, then the counter-current chromatographic method can be extended for use with gram quantities of dye. C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP WEISZ, A (reprint author), US FDA,DIV COLORS & COSMET,WASHINGTON,DC 20204, USA. NR 16 TC 10 Z9 10 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 157 EP 164 DI 10.1016/S0021-9673(01)91633-4 PG 8 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400018 PM 1646831 ER PT J AU CAHNMANN, HJ GONCALVES, E ITO, Y FALES, HM SOKOLOSKI, EA AF CAHNMANN, HJ GONCALVES, E ITO, Y FALES, HM SOKOLOSKI, EA TI SYNTHESIS AND CHARACTERIZATION OF N-BROMOACETYL-3,3',5-TRIIODO-L-THYRONINE SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID RECEPTOR; CELLS AB N-Bromoacetyl-3,3',5-triiodo-L-thyronine and carrier-free [3'-I-125]-N-bromoacetyl-3,3',5-triiodo-L-thyronine, to be used for affinity labeling of thyroid hormone receptors, were synthesized using a one-step procedure: a solution of the thyroid hormone 3,3',5-triiodo-L-thyronine and bromoacetyl bromide in ethyl acetate was refluxed for an optimal period of time which depends on the amount of hormone processed. The bromoacetylated hormone thus obtained was then fractionated by high-speed counter-current chromatography which yielded N-bromoacetyl-3,3',5-triiodo-L-thyronine that was pure by the criteria of high-performance liquid chromatography and thin-layer chromatography with different solvent systems. The pure product was well separated from all contaminants including one which in high-performance liquid chromatography was not easily separated from N-bromoacetyl-3,3',5-triiodo-L-thyronine. The latter was characterized by H-1 nuclear magnetic resonance, plasma desorption mass spectrometry, thin-layer chromatography, high-performance liquid chromatography, UV spectrophotometry, and melting point. Amounts of 3,3',5-triiodo-L-thyronine ranging from picograms, including carrier-free I-125-labeled triiodothyronine, to 200 to 300 mg can be processed with the equipment used in the present investigation. C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP CAHNMANN, HJ (reprint author), NIDDKD,BETHESDA,MD 20892, USA. NR 12 TC 5 Z9 5 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 165 EP 175 DI 10.1016/S0021-9673(01)91634-6 PG 11 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400019 PM 2050789 ER PT J AU APUD, JA ITO, Y AF APUD, JA ITO, Y TI SEMIPREPARATIVE PURIFICATION OF AN ENDOGENOUS LIGAND FOR BRAIN SEROTONIN-2 RECEPTORS BY COIL PLANET CENTRIFUGE COUNTERCURRENT CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID RECOGNITION SITES; SOLVENT SYSTEMS; KETANSERIN; MIANSERIN AB A horizontal flow-through coil planet centrifuge equipped with a rotatory frame holding three sets of composite column assemblies was used for purification of an endogenous ligand (ketanserin binding inhibitor) for the [H-3]-ketanserin (H-3-KET) recognition site. The protein mixture containing the endogenous material was successfully resolved by using a two-phase solvent system consisting of 95% ethanol-31.5% ammonium sulphate (1:2). The active fractions on H-3-KET binding obtained after counter-current chromatography (CCC) were further purified through a C18-mu-Bondapak reversed-phase high-pressure liquid chromatographic column. The introduction of this advanced CCC technique represents an important step in the application of CCC for the separation of polar proteins from protein mixtures. C1 NHLBI,BIOPHYS CHEM LAB,BLDG 10,ROOM 7N-322,BETHESDA,MD 20892. RP APUD, JA (reprint author), FIDIA GEORGETOWN INST NEUROSCI,4000 RESERVOIR RD NW,WASHINGTON,DC 20007, USA. NR 12 TC 10 Z9 10 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 177 EP 185 DI 10.1016/S0021-9673(01)91635-8 PG 9 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400020 PM 2050790 ER PT J AU HARADA, KI IKAI, Y YAMAZAKI, Y OKA, H SUZUKI, M NAKAZAWA, H ITO, Y AF HARADA, KI IKAI, Y YAMAZAKI, Y OKA, H SUZUKI, M NAKAZAWA, H ITO, Y TI ISOLATION OF BACITRACIN-A AND BACITRACIN-F BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID ANTIBIOTICS AB The major components of bacitracin were separated and purified using high-speed counter-current chromatography (HSCCC). A systematic search for optimum two-phase solvent systems resulted in two systems: chloroform-ethanol-methanol-water (5:3:3:4) and chloroform-ethanol-water (5:4:3). These were selected based on the determination of the partition coefficients of all the components and the settling time of the phases. HSCCC with these solvent systems separated two components, bacitracins A and F. Improvements in the flow-cell arrangement eliminated noise in detection, making in-line monitoring possible. A tandem mass spectrometric technique was used to characterize the isolated components. C1 NATL INST PUBL HLTH,MINATO KU,TOKYO 108,JAPAN. NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP HARADA, KI (reprint author), MEIJO UNIV,FAC PHARM,TEMPA KU,NAGOYA,AICHI 468,JAPAN. NR 22 TC 24 Z9 25 U1 1 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 203 EP 212 DI 10.1016/S0021-9673(01)91638-3 PG 10 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400023 PM 2050791 ER PT J AU OKA, H HARADA, KI SUZUKI, M NAKAZAWA, H ITO, Y AF OKA, H HARADA, KI SUZUKI, M NAKAZAWA, H ITO, Y TI FOAM COUNTERCURRENT CHROMATOGRAPHY OF BACITRACIN .2. CONTINUOUS REMOVAL AND CONCENTRATION OF HYDROPHOBIC COMPONENTS WITH NITROGEN GAS AND DISTILLED WATER FREE OF SURFACTANTS OR OTHER ADDITIVES SO JOURNAL OF CHROMATOGRAPHY LA English DT Article AB Foam counter-current chromatography has been successfully applied to continuous removal and concentration of hydrophobic bacitracin (BC) components from a large volume solution using nitrogen gas and distilled water free of surfactants or other additives. The experiment was initiated by introducing nitrogen at the head inlet of the coil rotated at 500 rpm. Then, a 2.5-1 volume of the sample solution containing BC at 50 ppm was continuously introduced into the middle portion of the coil at 1.5 ml/min. The hydrophobic components produced a thick foam which was carried with the gas phase and collected from the tail end of the coil while other components stayed in the liquid stream and eluted from the head outlet of the coil. High-performance liquid chromatographic analysis of the foam fraction revealed that the degree of enrichment increased with hydrophobicity of the BC components. BC-A and -F were enriched 1400 and 2260 times, respectively, compared with the original concentration in the sample solution. These results clearly indicate that the present method will be quite effective for detection and/or isolation of a small amount of natural products present in a large volume of aqueous solution. C1 NHLBI,BIOPHYS CHEM LAB,BLDG 10,ROOM 7N-322,BETHESDA,MD 20892. NHLBI,TECH DEV LAB,BETHESDA,MD 20892. MEIJO UNIV,FAC PHARM,NAGOYA,AICHI 468,JAPAN. INST PUBL HLTH,TOKYO 108,JAPAN. NR 3 TC 10 Z9 11 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 213 EP 218 DI 10.1016/S0021-9673(01)91639-5 PG 6 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400024 PM 2050792 ER PT J AU LI, P ZHANG, TY HUA, X ITO, Y AF LI, P ZHANG, TY HUA, X ITO, Y TI STUDIES ON THE PREPARATIVE CAPABILITY OF THE HORIZONTAL FLOW-THROUGH COIL PLANET CENTRIFUGE AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN THE SEPARATION OF POLAR COMPOUNDS FROM OXYTROPIS-OCHROCEPHALA BUNGE SO JOURNAL OF CHROMATOGRAPHY LA English DT Article ID SPEED COUNTERCURRENT CHROMATOGRAPHY; FLAVONOIDS; ALKALOIDS AB Horizontal flow-through coil planet centrifuge (CPC) and high-performance liquid chromatography (HPLC) techniques were used for separation of polar compounds from a crude ethanol extract of Oxytropis ochrocephala Bunge, a poisonous legume plant widely distributed in northwestern China. The performance of these two chromatographic methods was compared in terms of column efficiency, peak resolution, separation time, sample loading capacity, etc. The results indicated that two polar compounds in the crude extract were equally well separated by these two methods. HPLC gave comparable peak resolution in shorter separation time while its sample loading capacity was limited to the mg range. The CPC method required a long separation time, but yielded a higher purity of fractions with a much greater capacity. C1 NHLBI,BLDG 10,ROOM 7N-322,BETHESDA,MD 20892. ACAD SINICA,LANZHOU INST CHEM PHYS,LANZHOU 73000,PEOPLES R CHINA. BEIJING INST NEW TECHNOL APPLICAT,BEIJING 100035,PEOPLES R CHINA. NR 12 TC 6 Z9 6 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR PD JAN 18 PY 1991 VL 538 IS 1 BP 219 EP 225 DI 10.1016/S0021-9673(01)91640-1 PG 7 WC Chemistry, Analytical SC Chemistry GA EW734 UT WOS:A1991EW73400025 PM 2050793 ER PT J AU PATRICK, MA SETHI, SK UNGER, SE MIRSADEGHI, S RIBEIRO, AA THAKKER, DR AF PATRICK, MA SETHI, SK UNGER, SE MIRSADEGHI, S RIBEIRO, AA THAKKER, DR TI A NOVEL REACTION BETWEEN ACETONE AND THE BENZO[C]PHENANTHRENE K-REGION ORTHO-QUINONE CONTAINING A PERI-FLUORO SUBSTITUENT SO JOURNAL OF ORGANIC CHEMISTRY LA English DT Note ID CLAISEN REARRANGEMENT C1 GLAXO INC,DEPT DRUG METAB,RES TRIANGLE PK,NC 27709. GLAXO INC,DEPT ANALYT CHEM,RES TRIANGLE PK,NC 27709. NIH,BETHESDA,MD 20892. DUKE UNIV,MED CTR,DEPT RADIOL,DURHAM,NC 27710. DUKE UNIV,MED CTR,CTR NUCL MAGNET RESONANCE,DURHAM,NC 27710. NR 12 TC 1 Z9 1 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-3263 J9 J ORG CHEM JI J. Org. Chem. PD JAN 18 PY 1991 VL 56 IS 2 BP 888 EP 891 DI 10.1021/jo00002a080 PG 4 WC Chemistry, Organic SC Chemistry GA EU108 UT WOS:A1991EU10800080 ER PT J AU TITOMIROV, AV SUKHAREV, S KISTANOVA, E AF TITOMIROV, AV SUKHAREV, S KISTANOVA, E TI INVIVO ELECTROPORATION AND STABLE TRANSFORMATION OF SKIN CELLS OF NEWBORN MICE BY PLASMID DNA SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Note DE TRANSFORMATION; ELECTROPORATION INVIVO; MOUSE SKIN ID GENE-TRANSFER; RAT-LIVER; EXPRESSION; FRAGMENTS; ONCOGENES; THERAPY AB The skin cells of newborn mice were stably transformed in vivo with the aid of electroporation. The plasmid DNA was introduced subcutaneously followed by high-voltage pulses applied to the skin pleat. NEO-resistant colonies were found in primary cell cultures obtained from the treated skin. The experiments show that in vivo electroporation can be used for the introduction of plasmid DNA into skin cells of mouse. RP TITOMIROV, AV (reprint author), NICHHD,MAMMALIAN GENES & DEV LAB,BLDG 6,ROOM 338,BETHESDA,MD 20892, USA. NR 23 TC 289 Z9 296 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD JAN 17 PY 1991 VL 1088 IS 1 BP 131 EP 134 DI 10.1016/0167-4781(91)90162-F PG 4 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EW328 UT WOS:A1991EW32800017 PM 1703441 ER PT J AU BUTLER, KM HUSSON, RN BALIS, FM BROUWERS, P EDDY, J ELAMIN, D GRESS, J HAWKINS, M JAROSINSKI, P MOSS, H POPLACK, D SANTACROCE, S VENZON, D WIENER, L WOLTERS, P PIZZO, PA AF BUTLER, KM HUSSON, RN BALIS, FM BROUWERS, P EDDY, J ELAMIN, D GRESS, J HAWKINS, M JAROSINSKI, P MOSS, H POPLACK, D SANTACROCE, S VENZON, D WIENER, L WOLTERS, P PIZZO, PA TI DIDEOXYINOSINE IN CHILDREN WITH SYMPTOMATIC HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID AIDS-RELATED COMPLEX; PLACEBO-CONTROLLED TRIAL; AZIDOTHYMIDINE AZT; ZIDOVUDINE THERAPY; DOUBLE-BLIND; PHARMACOLOGY; TOXICITY; HIV AB Background. 2',3'-Dideoxyinosine (ddl) is a dideoxynucleoside with potent activity in vitro against the human immunodeficiency virus (HIV). In initial clinical trials in adults, ddl showed evidence of antiretroviral activity with little hematologic toxicity. Methods. We conducted a phase I-II study in 43 children with symptomatic (CDC class P-2) HIV infection. Of these children, 16 (median age, 10 years) had previously received zidovudine, and 27 (median age, 2.6 years) had not. ddl was administered orally in three divided doses totalling 60, 120, 180, 360, or 540 mg per square meter of body-surface area per day for 24 weeks. Eight of the 43 patients did not complete 24 weeks of ddl: Eight of the 43 patients did not complete 24 weeks of ddl: 6 died, 1 was withdrawn because of progressive disease, and the other because of toxicity. Results. After oral administration, ddl was rapidly absorbed, although its bioavailability varied greatly among patients. Pancreatitis developed in two children, one receiving ddl at each of the two highest doses. The median CD4 cell count in 38 patients with paired counts increased from 0.218 x 10(9) per liter (218 per cubic millimeter) at base line to 0.327 x 10(9) per liter (327 per cubic millimeter) after 20 to 24 weeks (P = 0.01). Those with CD4 cell counts above 01. x 10(9) per liter (100 per cubic millimeter) at base line were significantly more likely to improve in this respect. The median levels of p24 antigen (in 27 patients with detectable levels at entry) declined from 272 pg per milliliter at 20 to 24 weeks (P = 0.005). The plasma concentration of ddl correlated significantly with both the degree of decline in the p24 antigen and the degree of improvement in IQ score. Improvement in clinical and immunologic measures occurred in both the previously untreated patients and in those who had been treated with zidovudine. Conclusions. Dideoxyinosine was well tolerated and showed promising antiretroviral activity in HIV-infected children. The correlation between the clinical response and the plasma concentration of ddl indicates that bioavailability is an important consideration in the use of ddl to treat HIV infection and that individualized pharmacokinetic monitoring and dose adjustment may be important for optimal activity. C1 NCI,PEDIAT BRANCH,BLDG 10,RM 13N240,BETHESDA,MD 20892. NCI,BIOSTAT & DATA MANAGEMENT SECT,CLIN ONCOL PROGRAM,BETHESDA,MD 20892. NIH,CTR CLIN,DEPT PHARM,BETHESDA,MD 20892. MED ILLNESS COUNSELING,BETHESDA,MD. RI Venzon, David/B-3078-2008 NR 20 TC 207 Z9 207 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 17 PY 1991 VL 324 IS 3 BP 137 EP 144 DI 10.1056/NEJM199101173240301 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA ER471 UT WOS:A1991ER47100001 PM 1670591 ER PT J AU LOCKSHIN, MD AF LOCKSHIN, MD TI THERAPY FOR SYSTEMIC LUPUS-ERYTHEMATOSUS SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Editorial Material RP LOCKSHIN, MD (reprint author), NIAMSD,BETHESDA,MD 20892, USA. NR 11 TC 19 Z9 19 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 17 PY 1991 VL 324 IS 3 BP 189 EP 191 DI 10.1056/NEJM199101173240309 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA ER471 UT WOS:A1991ER47100009 PM 1984196 ER PT J AU HALL, WH AF HALL, WH TI TREATMENT OF EARLY-STAGE BREAST-CANCER SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP HALL, WH (reprint author), NIH,OFF MED APPLICAT RES,BLDG 1,ROOM 260,BETHESDA,MD 20892, USA. NR 0 TC 511 Z9 517 U1 1 U2 5 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JAN 16 PY 1991 VL 265 IS 3 BP 391 EP 395 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA ER256 UT WOS:A1991ER25600032 ER PT J AU OZMENT, JL SCHMIEDEKAMP, AM SCHULTZMERKEL, LA SMITH, RH MICHEJDA, CJ AF OZMENT, JL SCHMIEDEKAMP, AM SCHULTZMERKEL, LA SMITH, RH MICHEJDA, CJ TI THEORETICAL-ANALYSIS OF ACETYLTRIAZENE AND THE MECHANISTIC IMPLICATIONS OF ITS REACTION WITH ACID SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Article ID RING-SUBSTITUTED 3,3-DIMETHYL-1-PHENYLTRIAZENES; ALKYLDIAZONIUM IONS; TRIALKYLTRIAZENES; DECOMPOSITION; TRIAZENES; MUTAGENICITY; ABINITIO; RATS; AM1 AB Theoretical calculations have been carried out on the protonation of 3-acetyltriazene in order to provide insight into the mechanism of the acid-catalyzed decomposition of acyltriazenes. We have previously reported the results of calculations on a series of alkyltriazenes. Ab initio RHF calculations were carried out at the 3-21G level to determine the optimized SCF energies and geometries of the neutral molecule and some site-specific protonated species. This allowed an estimate of the proton affinity at each site. Experimental studies on alkyltriazenes indicated that initial protonation at the N3 site was critical in the acid catalysis mechanism, even though the calculated proton affinities indicated that N1 was a more basic site. In the case of the acyltriazenes, the calculations showed that the proton affinity at N3 was much lower than that at N1 or at the carbonyl oxygens. The geometrical changes produced by protonations at the various sites indicated that the N2-N3 bond shows a propensity for cleavage upon protonation at either N3 or at the carbonyl oxygen. These results suggest that acid-catalyzed decomposition of acetyltriazene would involve the breakage of the N2-N3 bond, rather than the hydrolysis of the acyl group. Subsequent experimental data supported this conclusion. A linear scaling method was applied to the geometric and energetic results from the semiempirical AM1 code to predict the results of the 3-21G calculations with a surprising degree of success. A predictor function to allow AM1 geometries and proton affinities to provide a good estimate of 3-21G results is given, and the limitations are discussed. C1 PENN STATE UNIV,DEPT PHYS,ABINGTON,PA 19001. NCI,FREDERICK CANC RES & DEV CTR,CHEM & PHYS CARCINOGENESIS LAB,ABL BASIC RES PROGRAM,FREDERICK,MD 21702. WESTERN MARYLAND COLL,DEPT CHEM,WESTMINSTER,MD 21157. RP OZMENT, JL (reprint author), PENN STATE UNIV,DEPT CHEM,OGONTZ CAMPUS,ABINGTON,PA 19001, USA. NR 26 TC 11 Z9 11 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD JAN 16 PY 1991 VL 113 IS 2 BP 397 EP 405 DI 10.1021/ja00002a003 PG 9 WC Chemistry, Multidisciplinary SC Chemistry GA ET682 UT WOS:A1991ET68200003 ER PT J AU FANANAPAZIR, L LEON, MB BONOW, RO TRACY, CM CANNON, RO EPSTEIN, SE AF FANANAPAZIR, L LEON, MB BONOW, RO TRACY, CM CANNON, RO EPSTEIN, SE TI SUDDEN-DEATH DURING EMPIRIC AMIODARONE THERAPY IN SYMPTOMATIC HYPERTROPHIC CARDIOMYOPATHY SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID CLINICAL MANIFESTATIONS; PATHO-PHYSIOLOGY; HEMODYNAMICS; INTERRELATIONS; ARRHYTHMIA; VERAPAMIL; EXERCISE AB Amiodarone is reported to improve symptoms and to prevent sudden death in patients with hypertrophic cardiomyopathy (HC). Amiodarone treatment (loading dose 30 g given over 6 weeks; maintenance dose 400 mg/day) was prospectively evaluated in 50 patients with HC in whom the drug was initiated because of symptoms refractory to conventional drug therapy (calcium antagonists and beta blockers). Twenty-one (42%) patients had ventricular tachycardia (VT) during Holter monitoring. Amiodarone significantly and often markedly improved the patients' New York Heart Association functional class status (from 3.3 to 2.7 at 2 months, p < 0.001) and treadmill exercise duration (p < 0.001). Eight patients, however, died (7 suddenly) during a mean follow-up period of 2.2 +/- 1.8 years. Of the 7 sudden deaths, 6 occurred within 5 months of initiation of treatment. The 6-month and 1- and 2-year survival rates were 87, 85 and 80%, respectively. The survival rate of patients with VT was significantly worse than that of patients without VT (61 vs 97% at 2 years; p < 0.01). Sudden death occurred despite abolition of VT on Holter monitoring. Amiodarone increased left ventricular peak filling rate by radionuclide angiography in 20 of 33 patients (61%) (p < 0.01). Decrease in peak left ventricular filling rate within 10 days of amiodarone therapy (8 of 33 patients) was associated with subsequent sudden death (p < 0.04). It is concluded that although amiodarone results in significant functional improvement in most symptomatic patients with HC and reduces VT on ambulatory monitoring, empiric therapy with this potent antiarrhythmic drug may result in a high early incidence of sudden death. Amiodarone may provoke malignant arrhythmias or conduction abnormalities in this subgroup of patients, particularly in patients in whom left ventricular diastolic filling is reduced by the drug. RP FANANAPAZIR, L (reprint author), NHLBI,CARDIOL BRANCH,BLDG 10,ROOM 7B-14,BETHESDA,MD 20892, USA. NR 22 TC 53 Z9 55 U1 0 U2 1 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD JAN 15 PY 1991 VL 67 IS 2 BP 169 EP 174 DI 10.1016/0002-9149(91)90440-V PG 6 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA ET065 UT WOS:A1991ET06500011 PM 1987718 ER PT J AU FANANAPAZIR, L EPSTEIN, SE AF FANANAPAZIR, L EPSTEIN, SE TI VALUE OF ELECTROPHYSIOLOGIC STUDIES IN HYPERTROPHIC CARDIOMYOPATHY TREATED WITH AMIODARONE SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID VENTRICULAR-TACHYCARDIA; CLINICAL MANIFESTATIONS; PATHO-PHYSIOLOGY; ARRHYTHMIAS; THERAPY; FIBRILLATION; TACHYARRHYTHMIAS; INTERRELATIONS; MANAGEMENT; RECURRENT AB The relation of electrophysiologic effects of amiodarone to long-term outcome was studied in 35 patients with hypertrophic cardiomyopathy (HC). Indications for electrophysiologic studies were: cardiac arrest (n = 3), syncope/presyncope (n = 27) and asymptomatic ventricular tachycardia (VT) (n = 5). Twenty-eight patients (80%) had VT, 3 (9%) atrial tachycardia and 3 (9%) paroxysmal atrial fibrillation during 24-hour Holter monitoring. The studies were repeated after a total amiodarone dose of 58 +/- 122 g and during a maintenance median daily dose of 400 mg. Amiodarone abolished paroxysmal atrial arrhythmias in all 6 patients. However, it caused marked atrioventricular nodal conduction abnormality in 3 patients and heart block or marked HV interval prolongation (to greater-than-or-equal-to 100 ms) in 4 patients. Sustained VT was induced in 26 patients (74%) at baseline study and in 23 patients (66%) taking amiodarone therapy. With amiodarone, VT was no longer inducible or was more difficult to induce in 11 patients (31%), and the drug abolished VT during Holter monitoring in all patients. However, VT was easier to induce with amiodarone or was induced only with amiodarone in 18 (51%) patients. Amiodarone significantly slowed the rate of induced VT (from 248 +/- 29 to 214 +/- 37 beats/min, p < 0.001). This was associated with a change in its morphology from polymorphic to monomorphic VT in 7 patients. During a follow up of 18 +/- 14 months (range 2 to 56), amiodarone was discontinued because of adverse effects in 8 patients (23%). Additionally, 4 of 18 patients in whom amiodarone facilitated induction of VT either died (n = 2) or received appropriate electric shocks from an implanted defibrillator device compared with none of the remaining 17 patients taking amiodarone in whom VT induction was not possible, unchanged or more difficult (1-year event-free rates: 71 vs 100%, p < 0.05). All 4 patients had presented with syncope or cardiac arrest. Thus, although amiodarone effectively suppresses atrial arrhythmias and reduces or prevents VT induction in about one-third of patients with HC, it causes important conduction abnormalities in about 20% of patients and facilitates induction of VT in about half of patients. This latter group of patients may be prone to malignant VT and amiodarone, and cannot be identified by Holter recordings. Electrophysiologic studies are therefore necessary when initiating amiodarone, because the results identify patients with HC who should receive a pacemaker with the drug, as well as those in whom amiodarone should be discontinued because of serious proarrhythmic effects. RP FANANAPAZIR, L (reprint author), NHLBI,CARDIOL BRANCH,CLIN ELECTROPHYSIOL LAB,BLDG 10,ROOM 7B-14,BETHESDA,MD 20892, USA. NR 27 TC 37 Z9 37 U1 0 U2 2 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD JAN 15 PY 1991 VL 67 IS 2 BP 175 EP 182 DI 10.1016/0002-9149(91)90441-M PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA ET065 UT WOS:A1991ET06500012 PM 1987719 ER PT J AU JOHN, EM SAVITZ, DA SANDLER, DP AF JOHN, EM SAVITZ, DA SANDLER, DP TI PRENATAL EXPOSURE TO PARENTS SMOKING AND CHILDHOOD-CANCER SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE BRAIN NEOPLASMS; CHILD; LEUKEMIA; SMOKING ID MATERNAL SMOKING; PASSIVE SMOKING; BRAIN-TUMORS; RISK-FACTORS; CIGARETTE-SMOKING; PREGNANCY; CHILDREN; SMOKERS; COTININE; LEUKEMIA AB The relation between parents' tobacco smoking prior to birth and cancer in the offspring was investigated with the use of data from a case-control study. Incident cases included all children (aged 0-14 years) diagnosed in Denver, Colorado from 1976 to 1983. Controls were selected through random digit dialing, and matched to cases on age, sex, and geographic area. Information on smoking by parents and other household members was obtained by personal interview for 223 cases and 196 controls. After adjustment for father's education, mother's smoking during the first trimester of pregnancy was associated with an increased risk for all cancers combined (odds ratio (OR) = 1.3, 95% confidence interval (Cl) 0.7-2.1), acute lymphocytic leukemia (OR = 1.9, 95% Cl 0.9-4.1), and lymphomas (OR = 2.3, 95% Cl 0.8-7.1). Adjusting for father's education, associations with father's smoking in the absence of mother's smoking were found for all cancers combined (OR = 1.2, 95% Cl 0.8-2.1), acute lymphocytic leukemia (OR = 1.4, 95% Cl 0.6-3.1), lymphomas (OR = 1.6, 95% 0.5-5.4), and brain cancer (OR = 1.6, 95% Cl 0.7-3.5). In spite of imprecision resulting from small numbers of cases in diagnostic subgroups, these results are suggestive of a possible influence of parents' smoking on childhood cancer. C1 UNIV N CAROLINA,SCH PUBL HLTH,DEPT EPIDEMIOL,CHAPEL HILL,NC 27514. NIEHS,DIV BIOMETRY & RISK ASSESSMENT,EPIDEMIOL BRANCH,RES TRIANGLE PK,NC 27709. OI Sandler, Dale/0000-0002-6776-0018 NR 41 TC 154 Z9 159 U1 0 U2 2 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JAN 15 PY 1991 VL 133 IS 2 BP 123 EP 132 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA ET755 UT WOS:A1991ET75500004 PM 1822074 ER PT J AU REED, D AF REED, D TI WARTIME DETERMINANTS OF ARTERIOGRAPHICALLY CONFIRMED CORONARY-ARTERY DISEASE IN BEIRUT SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Letter RP REED, D (reprint author), NHLBI,HONOLULU HEART PROGRAM,347 N KUAKINI ST,HONOLULU,HI 96817, USA. NR 1 TC 1 Z9 1 U1 0 U2 0 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JAN 15 PY 1991 VL 133 IS 2 BP 210 EP 211 PG 2 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA ET755 UT WOS:A1991ET75500014 PM 1985449 ER PT J AU SANDLER, DP AF SANDLER, DP TI BREAST-CANCER, CIGARETTE-SMOKING, AND PASSIVE SMOKING - COMMENT SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Letter RP SANDLER, DP (reprint author), NIEHS,EPIDEMIOL BRANCH,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JAN 15 PY 1991 VL 133 IS 2 BP 210 EP 210 PG 1 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA ET755 UT WOS:A1991ET75500013 ER PT J AU MOSELEY, MA DETERDING, LJ TOMER, KB JORGENSON, JW AF MOSELEY, MA DETERDING, LJ TOMER, KB JORGENSON, JW TI DETERMINATION OF BIOACTIVE PEPTIDES USING CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY SO ANALYTICAL CHEMISTRY LA English DT Article ID FAST-ATOM BOMBARDMENT; PERFORMANCE LIQUID-CHROMATOGRAPHY; ELECTROSPRAY IONIZATION INTERFACE; SULFONATED AZO DYES; GLASS-CAPILLARIES; FUSED-SILICA; FAB-MS; PROTEINS; BIOMOLECULES; ADSORPTION AB Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410 000 theoretical plates) were obtained from low femtomole levels of peptides. The analysis of basic peptides was accomplished by using aminopropyl-silylated CZE columns to minimize zone broadening due to adsorption effects. CZE/MS/MS data were acquired from femtomole levels of peptides in electrophoretic real time. C1 NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709. UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27514. RI Tomer, Kenneth/E-8018-2013 NR 54 TC 133 Z9 135 U1 0 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0003-2700 J9 ANAL CHEM JI Anal. Chem. PD JAN 15 PY 1991 VL 63 IS 2 BP 109 EP 114 DI 10.1021/ac00002a005 PG 6 WC Chemistry, Analytical SC Chemistry GA ER432 UT WOS:A1991ER43200005 PM 1812793 ER PT J AU DOLIN, R GRAHAM, BS GREENBERG, SB TACKET, CO BELSHE, RB MIDTHUN, K CLEMENTS, ML GORSE, GJ HORGAN, BW ATMAR, RL KARZON, DT BONNEZ, W FERNIE, BF MONTEFIORI, DC STABLEIN, DM SMITH, GE KOFF, WC AF DOLIN, R GRAHAM, BS GREENBERG, SB TACKET, CO BELSHE, RB MIDTHUN, K CLEMENTS, ML GORSE, GJ HORGAN, BW ATMAR, RL KARZON, DT BONNEZ, W FERNIE, BF MONTEFIORI, DC STABLEIN, DM SMITH, GE KOFF, WC TI THE SAFETY AND IMMUNOGENICITY OF A HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) RECOMBINANT GP160 CANDIDATE VACCINE IN HUMANS SO ANNALS OF INTERNAL MEDICINE LA English DT Article ID INFECTION; ANTIBODIES; NEUTRALIZATION; ASSAY; CD4 AB Objective: To evaluate the safety and immunogenicity of a human immunodeficiency virus type 1 (HIV-1) recombinant envelope glycoprotein (rgp160) candidate vaccine in humans. Subjects: Healthy adults (72) who were seronegative for HIV-1 were randomly assigned to one of four groups. Interventions: The subjects were randomly assigned to receive 40 or 80-mu-g of rgp160, 10-mu-g of hepatitis B vaccine, or placebo in three doses (on days 0, 30, and 180), with an elective, nonblinded administration of a fourth dose on day 540. Measurements and Main Results: Neither clinical nor laboratory toxicity was encountered during a follow-up period exceeding 21 months. No effect of immunization was noted on lymphocyte counts, mitogenic responses, or delayed-type hypersensitivity. Serum antibody responses to HIV envelope proteins detected by Western blot were seen in 30 of 33 subjects (91%; 95% CI, 71% to 97%) receiving either 40- or 80-mu-g doses of rgp160 and were most commonly of weakly reactive intensity. Responses were first noted by Western blot after the second dose. They markedly increased in frequency after the third dose and declined over the next 12 to 18 months. The administration of a fourth dose resulted in homologous neutralizing activity in sera from 5 of 24 subjects (21%; CI, 7% to 37%) as well as in complement-mediated antibody-dependent enhancement in sera from 6 of 24 subjects (25%; CI, 10% to 42%). Antibody responses were detected by enzyme-linked immunosorbent assay (ELISA) less frequently than by Western blot, and these responses persisted for a shorter time. Conclusions: The administration of rgp160 was well tolerated and safe, resulted in a high rate of antibody response by Western blot after the administration of the third and fourth doses, and generated serum neutralizing activity and complement-mediated antibody-dependent enhancement in some subjects after the fourth dose. C1 UNIV ROCHESTER,SCH MED & DENT,ROCHESTER,NY 14642. VANDERBILT UNIV,NASHVILLE,TN 37240. BAYLOR UNIV,HOUSTON,TX 77030. UNIV MARYLAND,BALTIMORE,MD 21201. ST LOUIS UNIV,SCH MED,ST LOUIS,MO 63104. JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,BALTIMORE,MD 21218. JOHNS HOPKINS UNIV,SCH MED,BALTIMORE,MD 21205. GEORGETOWN UNIV,ROCKVILLE,MD. EMMES CORP,POTOMAC,MD. MILROGENESYS INC,W HAVEN,CT. NIAID,AIDS,ROCKVILLE,MD. FU NIAID NIH HHS [N01-AI-62528, N01-AI-72624, N01-AI-52577] NR 26 TC 177 Z9 178 U1 3 U2 3 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD JAN 15 PY 1991 VL 114 IS 2 BP 119 EP 127 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA ER534 UT WOS:A1991ER53400004 PM 1984386 ER PT J AU SILVERMAN, SJ SHAW, JA CABIB, E AF SILVERMAN, SJ SHAW, JA CABIB, E TI PROTEINASE-B IS, INDEED, NOT REQUIRED FOR CHITIN SYNTHETASE-1 FUNCTION IN SACCHAROMYCES-CEREVISIAE SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID YEAST-CELL WALL; SEPTUM FORMATION; ACTIVATING FACTOR; ENZYME; PURIFICATION; DIVISION C1 NIDDKD,BIOCHEM & METAB LAB,BETHESDA,MD 20892. NR 19 TC 7 Z9 7 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD JAN 15 PY 1991 VL 174 IS 1 BP 204 EP 210 DI 10.1016/0006-291X(91)90506-3 PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA ET207 UT WOS:A1991ET20700032 PM 1989600 ER PT J AU TASAKI, I BYRNE, PM AF TASAKI, I BYRNE, PM TI DEMONSTRATION OF HEAT-PRODUCTION ASSOCIATED WITH SPREADING DEPRESSION IN THE AMPHIBIAN RETINA SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article RP TASAKI, I (reprint author), NIMH,CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 12 TC 11 Z9 11 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD JAN 15 PY 1991 VL 174 IS 1 BP 293 EP 297 DI 10.1016/0006-291X(91)90519-D PG 5 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA ET207 UT WOS:A1991ET20700045 PM 1989609 ER PT J AU AVISSAR, S MURPHY, DL SCHREIBER, G AF AVISSAR, S MURPHY, DL SCHREIBER, G TI MAGNESIUM REVERSAL OF LITHIUM INHIBITION OF BETA-ADRENERGIC AND MUSCARINIC RECEPTOR COUPLING TO G-PROTEINS SO BIOCHEMICAL PHARMACOLOGY LA English DT Article ID ADENYLATE-CYCLASE ACTIVITY; GUANINE-NUCLEOTIDES; AFFECTIVE-DISORDERS; CYCLIC-AMP; BRAIN; MG-2+; RAT; ACCUMULATION; ACTIVATION; MEMBRANES AB Recently, lithium was found to inhibit the coupling of both muscarinic cholinergic and beta-adrenergic receptors to pertussis toxin-sensitive and cholera toxin-sensitive G proteins respectively. These findings suggest that G proteins are the common site for both the antimanic and antidepressant therapeutic effects of lithium. Magnesium ions are crucial to the function of G proteins and interact with them at multiple sites. In the present study using rat cerebral cortex, we determined that magnesium can reverse the ability of lithium to inhibit isoprenaline- and carbamylcholine-induced increases in guanosine triphosphate (GTP) binding to G proteins. Lithium concentrations effective in attenuating G protein function were found to be hyperbolically dependent on free Mg2+ concentrations, suggesting multiple sites of competition between lithium and magnesium on G proteins. Free intracellular Mg2+ concentrations in rat cerebral cortex in vivo are known to be less than 1 mM. At such Mg2+ concentrations, therapeutically efficacious lithium concentrations (1 to 1.5 mM) were still able to alter G protein function, which supports the physiological and clinical relevance of lithium action on G proteins. C1 NIMH,CTR CLIN,CLIN SCI LAB,10-3D41,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 32 TC 51 Z9 51 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD JAN 15 PY 1991 VL 41 IS 2 BP 171 EP 175 DI 10.1016/0006-2952(91)90473-I PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EV522 UT WOS:A1991EV52200004 PM 1846545 ER PT J AU LITMAN, BJ LEWIS, EN LEVIN, IW AF LITMAN, BJ LEWIS, EN LEVIN, IW TI PACKING CHARACTERISTICS OF HIGHLY UNSATURATED BILAYER LIPIDS - RAMAN-SPECTROSCOPIC STUDIES OF MULTILAMELLAR PHOSPHATIDYLCHOLINE DISPERSIONS SO BIOCHEMISTRY LA English DT Article ID HIGHER-ORDER ANALYSIS; H STRETCHING REGION; POLYMETHYLENE CHAIN; ANISOTROPY DECAY; PHOSPHOLIPIDS; EQUILIBRIUM; MEMBRANE; VESICLES AB The thermotropic properties and acyl chain packing characteristics of multilamellar dispersions of highly unsaturated lipids were examined by Raman spectroscopy. Bilayer assemblies were composed of POPC (1-palmitoyl-2-oleoylphosphatidylcholine), PAPC (1-palmitoyl-2-arachidonylphosphatidylcholine), and PDPC (1-palmitoyl-2-docosahexaenoylphosphatidylcholine), lipid systems possessing saturated sn-1 chains and unsaturated sn-2 chains with one, four, and six double bonds, respectively. Raman spectra were recorded in the acyl chain 2800-3100-cm-1 carbon-hydrogen (C-H) stretching and 1100-1200-cm-1 carbon-carbon (C-C) stretching mode regions, spectral intervals reflecting both the inter- and intrachain order/disorder properties of the various lipid dispersions. In order to obtain C-H stretching mode spectra relevant solely to the sn-1 chains of PAPC and PDPC, liquid-phase spectra of arachidonic and docosahexaenoic acid, respectively, were subtracted from the observed phospholipid spectra. The unsaturated sn-2 chains of PAPC and PDPC undergo minimal conformational reorganizations as the bilayers pass from the gel to liquid-crystalline phases. Phase transition temperatures, T(m), derived from statistically fitting the temperature-dependent Raman spectral data are approximately -2.5, -22.5, and -3-degrees-C for POPC, PAPC, and PDPC, respectively. As the degree of unsaturation increases from POPC to PAPC and PDPC, the cooperativity of the phase transition, as measured by its breadth, decreases. Estimates of the transition widths from the temperature profiles are approximately 15-degrees-C for PAPC and 20-degrees-C for PDPC. The behavior of various Raman spectral parameters for the lipid gel phase reflects the formation of lateral microdomains, or clusters, whose packing properties maximize the van der Waals interactions between sn-1 chains. Since studies have shown that the conformational equilibrium of an integral membrane protein can be modulated by varying either the lipid sn-2 chain unsaturation or the bilayer temperature (Mitchell et al., 1990), the microdomain heterogeneity characteristic, for example, of highly unsaturated lipid bilayers may provide a mechanism for controlling the physical properties necessary for optimizing membrane functions associated with the structural reorganizations of integral proteins. C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. UNIV VIRGINIA,DEPT BIOCHEM,CHARLOTTESVILLE,VA 22908. FU NEI NIH HHS [EY00548] NR 23 TC 99 Z9 99 U1 0 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD JAN 15 PY 1991 VL 30 IS 2 BP 313 EP 319 DI 10.1021/bi00216a001 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET381 UT WOS:A1991ET38100001 PM 1988032 ER PT J AU SAMUNI, A GODINGER, D ARONOVITCH, J RUSSO, A MITCHELL, JB AF SAMUNI, A GODINGER, D ARONOVITCH, J RUSSO, A MITCHELL, JB TI NITROXIDES BLOCK DNA SCISSION AND PROTECT CELLS FROM OXIDATIVE DAMAGE SO BIOCHEMISTRY LA English DT Article ID HYDROGEN-PEROXIDE; ESCHERICHIA-COLI; SUPEROXIDE; COPPER; PARTICIPATION; COMPLEXES; RADICALS; OXYGEN; IRON AB The protective effect of cyclic stable nitroxide free radicals, having SOD-like activity, against oxidative damage was studied by using Esherichia coli xthA DNA repair-deficient mutant hypersensitive to H2O2. Oxidative damage induced by H2O2 was assayed by monitoring cell survival. The metal chelator 1,10-phenanthroline (OP), which readily intercalates into DNA, potentiated the H2O2-induced damage. The extent of in vivo DNA scission and degradation was studied and compared with the loss of cell viability. The extent of DNA breakage correlated with cell killing, supporting previous suggestions that DNA is the crucial cellular target of H2O2 cytotoxicity. The xthA cells were protected by catalase but not by superoxide dismutase (SOD). Both five- and six-membered ring nitroxides, having SOD-like activity, protected growing and resting cells from H2O2 toxicity, without lowering H2O2 concentration. To check whether nitroxides protect against O2.- -independent injury also, experiments were repeated under hypoxia. These nitroxides also protected hypoxic cells against H2O2, suggesting alternative modes of protection. Since nitroxides were found to reoxidize DNA-bound iron(II), the present results suggest that nitroxides protect by oxidizing reduced transition metals, thus interfering with the Fenton reaction. C1 NCI,DIV CANC TREATMENT,CLIN ONCOL PROGRAM,BETHESDA,MD 20892. RP SAMUNI, A (reprint author), HEBREW UNIV JERUSALEM,SCH MED,IL-91010 JERUSALEM,ISRAEL. NR 33 TC 103 Z9 103 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD JAN 15 PY 1991 VL 30 IS 2 BP 555 EP 561 DI 10.1021/bi00216a033 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET381 UT WOS:A1991ET38100033 PM 1846300 ER PT J AU BAKER, NJ KIRCH, DG WALDO, M BELL, J ADLER, LE HATTOX, S MURPHY, R FREEDMAN, R AF BAKER, NJ KIRCH, DG WALDO, M BELL, J ADLER, LE HATTOX, S MURPHY, R FREEDMAN, R TI PLASMA HOMOVANILLIC-ACID AND PROGNOSIS IN SCHIZOPHRENIA SO BIOLOGICAL PSYCHIATRY LA English DT Note ID NEUROLEPTIC TREATMENT; TARDIVE-DYSKINESIA; METABOLITES; WITHDRAWAL C1 VET ADM MED CTR,DEPT PSYCHIAT,DENVER,CO 80220. VET ADM MED CTR,DEPT PHARMACOL,DENVER,CO 80220. ST ELIZABETH HOSP,NIMH,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. RP BAKER, NJ (reprint author), UNIV COLORADO,HLTH SCI CTR,DEPT PSYCHIAT C268,4200 E 9TH AVE,DENVER,CO 80262, USA. NR 15 TC 9 Z9 9 U1 2 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD JAN 15 PY 1991 VL 29 IS 2 BP 192 EP 196 DI 10.1016/0006-3223(91)90048-Q PG 5 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA ET856 UT WOS:A1991ET85600011 PM 1671646 ER PT J AU BASTA, M FRIES, LF FRANK, MM AF BASTA, M FRIES, LF FRANK, MM TI HIGH-DOSES OF INTRAVENOUS IG INHIBIT INVITRO UPTAKE OF C4 FRAGMENTS ONTO SENSITIZED ERYTHROCYTES SO BLOOD LA English DT Article ID IDIOPATHIC THROMBOCYTOPENIC PURPURA; MEDIATED INVIVO CLEARANCE; GAMMA-GLOBULIN; IMMUNOGLOBULIN; REVERSAL; SERUM RP BASTA, M (reprint author), NIAID,CLIN INVEST LAB,BLDG 10,ROOM 11N228,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. OI Basta, Milan/0000-0001-5958-9241 NR 16 TC 92 Z9 92 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD JAN 15 PY 1991 VL 77 IS 2 BP 376 EP 380 PG 5 WC Hematology SC Hematology GA ER719 UT WOS:A1991ER71900022 PM 1985703 ER PT J AU HALL, WH AF HALL, WH TI SURGERY FOR EPILEPSY - SUMMARY OF A CONSENSUS STATEMENT SO CANADIAN MEDICAL ASSOCIATION JOURNAL LA English DT Editorial Material RP HALL, WH (reprint author), NIH,OFF MED APPLICAT RES,BLDG 1,RM 259,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CANADIAN MEDICAL ASSOCIATION PI OTTAWA PA 1867 ALTA VISTA DR, OTTAWA ON K1G 3Y6, CANADA SN 0820-3946 J9 CAN MED ASSOC J JI Can. Med. Assoc. J. PD JAN 15 PY 1991 VL 144 IS 2 BP 145 EP 146 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA EU271 UT WOS:A1991EU27100030 ER PT J AU RATKO, TA DETRISAC, CJ MEHTA, RG KELLOFF, GJ MOON, RC AF RATKO, TA DETRISAC, CJ MEHTA, RG KELLOFF, GJ MOON, RC TI INHIBITION OF RAT MAMMARY-GLAND CHEMICAL CARCINOGENESIS BY DIETARY DEHYDROEPIANDROSTERONE OR A FLUORINATED ANALOG OF DEHYDROEPIANDROSTERONE SO CANCER RESEARCH LA English DT Article ID LONG-TERM TREATMENT; 12-O-TETRADECANOYLPHORBOL-13-ACETATE STIMULATION; BREAST-CANCER; MICE; GROWTH; PROLACTIN; SULFATE; HORMONE; TUMORS; SERUM AB The chemopreventive efficacy of p.o. administered dehydroepiandrosterone (DHEA), DHEA plus N-(4-hydroxyphenyl)retinamide (4-HPR), or 16-alpha-fluoro-5-androsten-17-one (DHEA analogue 8354) was examined in rats treated with N-methyl-N-nitrosourea (MNU; 50 mg/kg body weight, i.v.) at 50 days of age. Semipurified diet (AIN-76A) containing each steroid alone, or DHEA plus 4-HPR, was administered during initiation (-1 week to +1 week post-MNU), promotion/progression (+1 week post-MNU to termination), or both phases (-1 week post-MNU to termination) of the carcinogenic process. Neither DHEA nor DHEA analogue 8354 (0.2%, w/w) significantly affected the initiation of mammary cancer when administered alone; however, DHEA (0.2%, w/w) plus 4-HPR (1 mmol/kg diet) significantly reduced cancer multiplicity (26%) when given during initiation. All three treatments were strongly effective when given during promotion/progression, significantly reducing mammary cancer multiplicity by 77% (DHEA), 84% (DHEA/4-HPR), and 66% (DHEA analogue 8354), relative to carcinogen controls. Cancer incidence was significantly inhibited by DHEA (33% inhibition) and DHEA/4-HPR (24% reduction) during promotion/progression. However, the most effective chemopreventive treatment encompassed both phases of carcinogenesis. Thus, under these conditions, DHEA (0.2% or 0.1%, w/w) reduced cancer incidence (52% and 32% reductions, respectively) and multiplicity (91% and 86% reductions, respectively). Further reduction in mammary cancer incidence was observed in animals that received DHEA (both doses) plus 4-HPR (1 and 0.5 mmol/kg diet, respectively). DHEA analogue 8354 (0.2% or 0.1%, w/w) given for the duration of the study reduced only cancer multiplicity (61% and 56% reductions, respectively). Tumor-related mortality was significantly lower in rats that received long-term treatment with DHEA or DHEA/4-HPR, when compared with carcinogen controls. Except for a slight, but significant, postcarcinogen decrease in the mean body weights of rats treated concomitantly with DHEA (plus or minus 4-HPR) and MNU, additional gross manifestations of steroid-induced toxicity were not observed. C1 IIT,RES INST,DIV LIFE SCI,BIOMED SCI LAB,10 W 35TH ST,CHICAGO,IL 60616. NCI,CHEMOPREVENT BRANCH,BETHESDA,MD 20892. FU NCI NIH HHS [CA-34664, N01-CN-45912-14] NR 35 TC 79 Z9 80 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 15 PY 1991 VL 51 IS 2 BP 481 EP 486 PG 6 WC Oncology SC Oncology GA ET789 UT WOS:A1991ET78900004 PM 1824682 ER PT J AU SAMUELS, BL MURRAY, JL COHEN, MB SAFA, AR SINHA, BK TOWNSEND, AJ BECKETT, MA WEICHSELBAUM, RR AF SAMUELS, BL MURRAY, JL COHEN, MB SAFA, AR SINHA, BK TOWNSEND, AJ BECKETT, MA WEICHSELBAUM, RR TI INCREASED GLUTATHIONE-PEROXIDASE ACTIVITY IN A HUMAN SARCOMA CELL-LINE WITH INHERENT DOXORUBICIN RESISTANCE SO CANCER RESEARCH LA English DT Article ID BREAST CANCER-CELLS; ASCITES TUMOR-CELLS; MULTIDRUG-RESISTANCE; DRUG-RESISTANCE; P-GLYCOPROTEIN; CROSS-RESISTANCE; DAUNORUBICIN; MECHANISM; VINCRISTINE; TRANSFERASE AB Several mechanisms of drug resistance have been defined using cell lines selected for resistance in vitro. However, the relevance of these to tumor cell resistance in vivo remains unclear. We established tumor cell lines from biopsies of human sarcomas before and after doxorubicin therapy. One pretreatment sarcoma line, STSAR90, was 6-fold less sensitive to doxorubicin than was a normal fibroblast line, AG1522. The sensitivities of six other sarcoma lines were similar to that of AG1522. STSAR90 cells did not overexpress P-glycoprotein mRNA, by Northern analysis with the pCHP1 complementary DNA fragment. Photoaffinity labeling with the vinblastine analogue N-(p-azido-3-I-125-salicyl)-N'-beta-aminoethylvindesine did not show increased P-glycoprotein concentrations. Accumulation of [H-3]daunomycin was not decreased in STSAR90 compared with a less resistant sarcoma line, STSAR11, nor was the doxorubicin sensitivity of STSAR90 increased by coincubation with verapamil. Glutathione levels were twice as high in STSAR90 as in STSAR11, and glutathione peroxidase activity was 3.5- to 6-fold higher. This was due mostly to an increase in selenium-dependent peroxidase activity. After exposure to doxorubicin, STSAR90 cells formed only half as much measurable hydroxyl radical as STSAR11, as detected by electron spin resonance spectrometry. Doxorubicin sensitivity was increased in STSAR90 cells when intracellular glutathione levels were reduced by buthionine sulfoximine. These results indicate that multidrug resistance due to P-glycoprotein-mediated drug efflux is not the only mechanism of doxorubicin resistance that occurs in sarcomas and that glutathione peroxidase-dependent detoxification of doxorubicin-induced oxygen radicals may contribute to clinical doxorubicin resistance. C1 UNIV CHICAGO,DEPT RADIAT ONCOL,CHICAGO,IL 60637. NCI,MED BRANCH,BETHESDA,MD 20892. RP SAMUELS, BL (reprint author), UNIV CHICAGO,HEMATOL ONCOL SECT,5841 S MARYLAND AVE,CHICAGO,IL 60637, USA. FU NCI NIH HHS [CA 47652, CA 42596] NR 32 TC 73 Z9 74 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 15 PY 1991 VL 51 IS 2 BP 521 EP 527 PG 7 WC Oncology SC Oncology GA ET789 UT WOS:A1991ET78900011 PM 1845955 ER PT J AU LEYLANDJONES, BR TOWNSEND, AJ TU, CPD COWAN, KH GOLDSMITH, ME AF LEYLANDJONES, BR TOWNSEND, AJ TU, CPD COWAN, KH GOLDSMITH, ME TI ANTINEOPLASTIC DRUG SENSITIVITY OF HUMAN MCF-7 BREAST-CANCER CELLS STABLY TRANSFECTED WITH A HUMAN ALPHA CLASS GLUTATHIONE-S-TRANSFERASE GENE SO CANCER RESEARCH LA English DT Article ID NITROGEN MUSTARDS; HUMAN-LIVER; SEPARATE GENES; CDNA CLONE; NUCLEOTIDE-SEQUENCE; MAMMALIAN-CELLS; MESSENGER-RNA; SUBUNIT CDNA; TUMOR-CELLS; EXPRESSION AB Studies have suggested that the alpha class glutathione S-transferase (GST) may protect cells from the chemotherapeutic drugs chlorambucil and melphalan. In order to further define the function of human alpha class GST, a complementary DNA which encodes it was ligated into an expression vector under the direction of the human metallothionein-II(A) promoter and stably transfected into human MCF-7 breast cancer cells in conjunction with the G418-selectable plasmid pSV2neo. Clonal cell lines were identified which expressed increased levels of GST enzyme activity (2.2- to 5.6-fold). The transfected cell lines also had increased peroxidase activity using cumene hydroperoxide as the substrate (1.9- to 3.8-fold) which is consistent with the intrinsic peroxidase activity of alpha class GSTs. Southern blot analysis indicated that genomic DNA from these cells contained a fragment indistinguishable from the transfected alpha class GST complementary DNA (850 base pairs); Northern blot analysis of total cellular RNA indicated that these cells contained appropriately sized alpha class GST RNA (980 nucleotides); and Western blot analysis indicated that, while MCF-7 cells contained no detectable alpha class GST protein, the transfected cells contained markedly elevated levels of alpha class GST but no detectable mu or pi class GST. These alpha class GST transfected cells had increased resistance to ethacrynic acid (2.1- to 3.0-fold). However, the transfected cells failed to show any increased resistance measured at the drug dosage which inhibited 50% of the colony formation to the chemotherapeutic drugs chlorambucil, melphalan, Adriamycin, or cisplatin under conditions of either continuous or 1-h drug exposure. Neither was there any change in sensitivity to the cytotoxins benzo(a)pyrene, benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (anti), or 1-chloro-2,4-dinitrobenzene. These studies indicate that expression of this human alpha class GST by itself in MCF-7 human breast cancer cells does not confer resistance to the chemotherapeutic drugs tested under the conditions used in these studies. C1 NCI,MED BRANCH,BLDG 10,RM 12N226,BETHESDA,MD 20892. PENN STATE UNIV,DEPT MOLEC & CELL BIOL,UNIVERSITY PK,PA 16802. NR 66 TC 68 Z9 68 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 15 PY 1991 VL 51 IS 2 BP 587 EP 594 PG 8 WC Oncology SC Oncology GA ET789 UT WOS:A1991ET78900020 PM 1985777 ER PT J AU MULSHINE, JL CARRASQUILLO, JA WEINSTEIN, JN KEENAN, AM REYNOLDS, JC HERDT, J BUNN, PA SAUSVILLE, E EDDY, J COTELINGAM, JD PERENTESIS, P PINSKY, C LARSON, SM AF MULSHINE, JL CARRASQUILLO, JA WEINSTEIN, JN KEENAN, AM REYNOLDS, JC HERDT, J BUNN, PA SAUSVILLE, E EDDY, J COTELINGAM, JD PERENTESIS, P PINSKY, C LARSON, SM TI DIRECT INTRALYMPHATIC INJECTION OF RADIOLABELED IN-111 T101 IN PATIENTS WITH CUTANEOUS T-CELL LYMPHOMA SO CANCER RESEARCH LA English DT Article ID T101 MONOCLONAL-ANTIBODY; CHRONIC LYMPHOCYTIC-LEUKEMIA; SEZARY SYNDROME; MYCOSIS-FUNGOIDES; IMMUNOLYMPHOSCINTIGRAPHY; RADIOIMMUNODETECTION; IMMUNOGLOBULIN; LYMPHATICS; THERAPY; LYMPHOSCINTIGRAPHY AB Direct intralymphatic administration of radiolabeled monoclonal antibody in targeting antigen-bearing lymphoma cells in regional lymph nodes of patients with cutaneous T-cell lymphoma was evaluated. Seven consecutive patients undergoing staging lymphangiography received intralymphatic infusions of In-111-T101 to evaluate lymph node involvement. This procedure was accomplished without significant complication. The In-111-T101 rapidly distributed throughout the regional lymphatic compartment and passed into the systemic circulation. Tumor-bearing sites in the inguinal-femoral lymph nodes retained from 0.42 to 4.8% of the injected dose of radiolabeled antibody. Three patients were upstaged to Stage IVA based on tumor involvement found after radiolymphoscintigraphy-directed biopsy of groin lymph nodes, selected because of intense radioactivity by gamma camera imaging. Compared with previously reported s.c. antibody administration, there was a marked reduction in the radioactive exposure of normal tissues at the injection sites in the lower extremities. Direct intralymphatic delivery of In-111-T101 appears to be a feasible, efficient method for delivering therapeutic doses of radiolabeled antibody. C1 NIH,WARREN C MAGNUSON CLIN CTR,DEPT NUCL MED,BETHESDA,MD 20892. NIH,WARREN C MAGNUSON CLIN CTR,DEPT RADIOL,BETHESDA,MD 20892. NCI,MATH BIOL LAB,BETHESDA,MD 20892. UNIV COLORADO,CTR CANC,DIV MED ONCOL,DENVER,CO 80262. UNIV COLORADO,CTR HLTH SERV,DENVER,CO 80262. USN HOSP,DEPT PATHOL,BETHESDA,MD 20814. NCI,FREDERICK CANC RES FACIL,DIV CANC TREATMENT,BIOL RESPONSE MODIFIER PROGRAM,FREDERICK,MD 21701. MEM SLOAN KETTERING CANC CTR,DEPT NUCL MED,NEW YORK,NY 10021. RP MULSHINE, JL (reprint author), USN HOSP,NCI NAVY MED ONCOL BRANCH,DIV CANC THERAPY,CLIN ONCOL PROGRAM,BETHESDA,MD 20814, USA. RI Carrasquillo, Jorge/E-7120-2010 NR 36 TC 13 Z9 13 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 15 PY 1991 VL 51 IS 2 BP 688 EP 695 PG 8 WC Oncology SC Oncology GA ET789 UT WOS:A1991ET78900037 PM 1985786 ER PT J AU DECLUE, JE VASS, WC PAPAGEORGE, AG LOWY, DR WILLUMSEN, BM AF DECLUE, JE VASS, WC PAPAGEORGE, AG LOWY, DR WILLUMSEN, BM TI INHIBITION OF CELL-GROWTH BY LOVASTATIN IS INDEPENDENT OF RAS FUNCTION SO CANCER RESEARCH LA English DT Article ID MURINE SARCOMA-VIRUS; MEVALONIC ACID; MONOCLONAL-ANTIBODIES; GENE FAMILY; SRC-GENE; PROTEINS; PRODUCT; P21RAS; TRANSFORMATION; MUTAGENESIS AB We have investigated the inhibition of cell growth by lovastatin (previously known as mevinolin), an antagonist of hydroxymethylglutaryl coenzyme A reductase which blocks the processing and membrane localization of ras proteins via inhibition of polyisoprenylation. A series of NIH 3T3 cells transformed by oncogenes with activities that are dependent or independent of isoprenylated ras were studied, including cells transformed by myristylated ras protein that is isoprenylation independent. Treatment with lovastatin at concentrations ranging from 5 to 15-mu-M for up to 96 h resulted in a time- and dose-dependent inhibition of cell growth in all lines tested. The inhibition ranged from 25 to 50% when cells were treated with 5-mu-M lovastatin for 48 h, to 72-90% for cells treated with 15-mu-M lovastatin for 96 h. Cells transformed by c-ras, v-ras, v-src, v-raf, and the myristylated ras genes displayed similar sensitivities; the parental NIH 3T3 line was the most resistant of the lines tested. Metabolic labeling of control and lovastatin-treated cells with [S-35]methionine or tritiated lipids revealed that 15-mu-M lovastatin blocked the processing of both endogenous ras and v-ras proteins yet had no effect on the lipidation of myristylated ras proteins. Addition of 300-mu-M mevalonic acid overcame the inhibition induced by 15-mu-M lovastatin. Thus the inhibition of cell growth in vitro by lovastatin did not show specificity for cells the transformation of which is dependent upon isoprenylated ras protein. It is therefore likely that the inhibition of other pathways affected by lovastatin, such as cholesterol biosynthesis or the processing of other cellular proteins, are responsible for the growth inhibition by lovastatin. C1 UNIV COPENHAGEN,INST MICROBIOL,DK-1353 COPENHAGEN,DENMARK. RP DECLUE, JE (reprint author), NCI,CELLULAR ONCOL LAB,BETHESDA,MD 20892, USA. RI Willumsen, Berthe/H-1903-2012 OI Willumsen, Berthe/0000-0002-2277-6999 NR 35 TC 140 Z9 143 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 15 PY 1991 VL 51 IS 2 BP 712 EP 717 PG 6 WC Oncology SC Oncology GA ET789 UT WOS:A1991ET78900040 PM 1985788 ER PT J AU HOTH, DF MYERS, MW AF HOTH, DF MYERS, MW TI CURRENT STATUS OF HIV THERAPY .1. ANTIRETROVIRAL AGENTS SO HOSPITAL PRACTICE LA English DT Article AB Zidovudine has proved to be an important palliative agent in all stages of HIV infection. It delays progression of disease in patients with asymptomatic or mildly symptomatic infection and decreases the frequency and severity of opportunistic disease in those with AIDS. The search for other, more effective antiretroviral agents goes on, with some promising possibilities. RP HOTH, DF (reprint author), NIAID,DIV AIDS,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MCGRAW HILL HEALTHCARE PUBLICATIONS PI MINNEAPOLIS PA 4530 WEST 77TH ST, MINNEAPOLIS, MN 55435-5000 SN 8750-2836 J9 HOSP PRACT JI Hosp. Pract. PD JAN 15 PY 1991 VL 26 IS 1 BP 174 EP & PG 0 WC Medicine, General & Internal SC General & Internal Medicine GA EV007 UT WOS:A1991EV00700015 PM 1702791 ER PT J AU ALSTON, K ROBINSON, RC PARK, SS GELBOIN, HV FRIEDMAN, FK AF ALSTON, K ROBINSON, RC PARK, SS GELBOIN, HV FRIEDMAN, FK TI INTERACTIONS AMONG CYTOCHROMES-P-450 IN THE ENDOPLASMIC-RETICULUM - DETECTION OF CHEMICALLY CROSS-LINKED COMPLEXES WITH MONOCLONAL-ANTIBODIES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID RAT-LIVER MICROSOMES; CYTOCHROME-P-450; METABOLISM; LINKING; P-450; PURIFICATION; ENZYMES; FORMS; SEX AB The quaternary structure of rat liver cytochrome P-450 within microsomal membranes from 3-methylcholanthrene-treated rats was examined by a novel chemical cross-linking-monoclonal antibody approach. Complex formation among the different forms of P-450 was probed by cross-linking of membrane proteins followed by immunopurification with a monoclonal antibody (mAb) to P-450c, the major 3-methylcholanthrene-inducible form. Subsequent immunoblot analysis of the immunopurified proteins with this mAb indicated that P-450c formed complexes with other microsomal proteins. Immunoblots with mAbs to different P-450s were carried out to identify the P-450s that were cross-linked to P-450c. This approach detected specific cross-linking of P-450c to P-450 2a. Immunoinhibition experiments suggest that P-450 2a further metabolizes the primary phenols produced by P-450c-catalyzed hydroxylation of benzo[alpha]pyrene. Complex formation among membrane-bound enzymes has implications for their catalytic efficiency and an approach combining cross-linking and monoclonal antibody-based characterization of cross-linked proteins will be useful for elucidating such membrane protein macrostructures. C1 NCI,MOLEC CARCINOGENESIS LAB,BLDG 37,RM 3E24,BETHESDA,MD 20892. BENEDICT COLL,DEPT CHEM,COLUMBIA,SC 29204. RI Friedman, Fred/D-4208-2016 FU NCRR NIH HHS [CRR08112] NR 37 TC 55 Z9 57 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 15 PY 1991 VL 266 IS 2 BP 735 EP 739 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET177 UT WOS:A1991ET17700012 PM 1985961 ER PT J AU DOEGE, KJ SASAKI, M KIMURA, T YAMADA, Y AF DOEGE, KJ SASAKI, M KIMURA, T YAMADA, Y TI COMPLETE CODING SEQUENCE AND DEDUCED PRIMARY STRUCTURE OF THE HUMAN CARTILAGE LARGE AGGREGATING PROTEOGLYCAN, AGGRECAN - HUMAN-SPECIFIC REPEATS, AND ADDITIONAL ALTERNATIVELY SPLICED FORMS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID LYMPHOCYTE HOMING RECEPTOR; AMINO-ACID-SEQUENCES; LINK PROTEIN; CORE PROTEIN; CDNA CLONES; MOLECULAR-CLONING; DOMAINS; GENE; RAT; ADHESION AB We have obtained the complete coding sequence of the large aggregating chondroitin sulfate proteoglycan of human cartilage (aggrecan) from a combination of cDNA and genomic exon sequencing. We screened a human costal chondrocyte cDNA library, using rat aggrecan cDNA probes, and obtained three nonoverlapping clones totaling 6.2 kilobases in length. These clones were sequenced, and the sequence of the gaps between clones was obtained from genomic exon fragments and polymerase chain reaction-amplified cDNA. The composite sequence is 7137 nucleotides long, encoding 2316 amino acids. The human and rat aggrecan amino acid sequences are about 75% identical, with domains ranging from 100% to about 60% of conserved amino acids. The human sequence contains two regions of highly conserved repeats not found in rat aggrecan: 11 repeats of a hexameric sequence in the keratan sulfate attachment domain, E-E-P-(S,F)-P-S; and a 19-amino acid sequence reiterated 19 times, in the CS-1 portion of the serine-glycine-containing region. There are at least three forms of aggrecan transcripts, generated by alternative exon usage, and the form reported here is the shortest and also the most prevalent, lacking both the epidermal growth factor-like domain, and the complement regulatory protein-like sequence. C1 OREGON HLTH SCI UNIV,DEPT BIOCHEM & MOLEC BIOL,PORTLAND,OR 97201. NATL BEPPU HOSP,BEPPU,OITA 87401,JAPAN. OSAKA UNIV,SCH MED,DEPT ORTHOPED SURG,OSAKA 553,JAPAN. NIDR,DEV BIOL & ANOMALIES LAB,BETHESDA,MD 20892. RP DOEGE, KJ (reprint author), OREGON HLTH SCI UNIV,SHRINERS HOSP CRIPPLED CHILDREN,3101 SW SAM JACKSON PK RD,PORTLAND,OR 97201, USA. NR 55 TC 364 Z9 368 U1 1 U2 4 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 15 PY 1991 VL 266 IS 2 BP 894 EP 902 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET177 UT WOS:A1991ET17700036 PM 1985970 ER PT J AU DESVERGNE, B PETTY, KJ NIKODEM, VM AF DESVERGNE, B PETTY, KJ NIKODEM, VM TI FUNCTIONAL-CHARACTERIZATION AND RECEPTOR-BINDING STUDIES OF THE MALIC ENZYME THYROID-HORMONE RESPONSE ELEMENT SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID GENE PROMOTER ACTIVITY; EXPRESSION; PROTEIN; TRANSCRIPTION; INDUCTION; SEQUENCE; SITE AB We previously showed that the 5'-flanking region of the malic enzyme (ME) gene contains a cis-regulatory element (-281 to -261) that binds thyroid hormone receptors and confers triiodothyronine (T3) inducibility of transcription to the ME promoter (Petty, K. J., Desvergne, B., Mitsuhashi, T., and Nikodem, V. M. (1990) J. Biol. Chem. 265, 7395-7400). In this report, we have used deletion and mutation analyses of the ME thyroid hormone response element (TRE) to evaluate the roles of several subregions of TRE in T3 binding and transactivation. ME TRE was shown to act as an enhancer conferring T3 responsiveness to a heterologous promoter thymidine kinase. Although T3 treatment induced the promoter activity, the absence of hormone resulted in repression as measured by the level of chloramphenicol acetyltransferase expression in the NIH 3T3 transient expression system in the presence of overexpressed receptor. The degree of repression was similar to the degree of T3 induction observed for the same TRE mutants. Mutation and deletion analyses indicated that the functional TRE is comprised of discrete regions that are not contiguous, with a dominant role of a cluster of G residues and an AGGACA sequence. Both functions, induction and repression of transcription, correlated with receptor binding to the ME TRE as determined by competition binding assays using wild type and mutated TRE as competitors. RP DESVERGNE, B (reprint author), NIDDKD,CLIN ENDOCRINOL BRANCH,BETHESDA,MD 20892, USA. RI Desvergne, Beatrice/C-8892-2016 OI Desvergne, Beatrice/0000-0001-5483-288X NR 34 TC 113 Z9 113 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 15 PY 1991 VL 266 IS 2 BP 1008 EP 1013 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET177 UT WOS:A1991ET17700054 PM 1985929 ER PT J AU RITTER, JK CRAWFORD, JM OWENS, IS AF RITTER, JK CRAWFORD, JM OWENS, IS TI CLONING OF 2 HUMAN LIVER BILIRUBIN UDP-GLUCURONOSYL-TRANSFERASE CDNAS WITH EXPRESSION IN COS-1 CELLS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID GUNN-RAT; GLUCURONYLTRANSFERASE; SEQUENCE; SPECIFICITY; JAUNDICE; FORM; ACID AB We report the isolation and characterization of two human liver cDNA clones, HUG-Br1 and HUG-Br2; each encodes a UDP-glucuronosyltransferase enzyme which glucuronidates bilirubin IX-alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and a diconjugate. HUG-Br1 cDNA (2351 base pairs) and HUG-Br2 cDNA (2368 base pairs) encode proteins with 533 and 534 amino acid residues, respectively, with a typical membrane-insertion signal peptide, membrane-spanning domain, and 3 or 5 potential asparagine-linked glycosylation sites. At the nucleic acid and deduced amino acid sequence levels the two clones are 82% similar overall, 66% similar in the amino termini, and identical after codon 287, thus encoding proteins with the same carboxyl terminus. The mRNA encoding HUG-Br1 is of high abundance, and the one encoding HUG-Br2 is of low abundance; both are 2.6 kilobases in length. Both messages (2.6 kilobases) were present in the explanted liver of a Type I Crigler-Najjar patient, although the level for that of HUG-Br1 was reduced 4.5-fold. Northern blot analysis of poly(A)+ RNA isolated from the liver of an untreated and a phenobarbital-treated Erythrocebus patas monkey with 5'-specific probes for each clone indicated that the HUG-Br2-encoded message is induced two fold, but that for HUG-Br1 is not. These data indicate that bilirubin is glucuronidated by at least two different proteins, most likely present in very different amounts. These cDNAs which encode functional bilirubin UDP-glucuronosyltransferases will allow the isolation of an appropriate gene to develop a gene therapy model for patients which have the totally deficient trait. C1 NICHHD,HUMAN GENET BRANCH,GENET DISORDERS DRUG METAB SECT,BLDG 10,RM 9S-242,BETHESDA,MD 20892. BRIGHAM & WOMENS HOSP,DEPT PATHOL,BOSTON,MA 02115. NR 31 TC 243 Z9 250 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 15 PY 1991 VL 266 IS 2 BP 1043 EP 1047 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET177 UT WOS:A1991ET17700059 PM 1898728 ER PT J AU JIMENEZ, M TSUKAMOTO, K HEARING, VJ AF JIMENEZ, M TSUKAMOTO, K HEARING, VJ TI TYROSINASES FROM 2 DIFFERENT LOCI ARE EXPRESSED BY NORMAL AND BY TRANSFORMED MELANOCYTES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ENCODING MOUSE TYROSINASE; MELANOMA CELL-CULTURES; MAMMALIAN TYROSINASE; STIMULATING HORMONE; DOPACHROME OXIDOREDUCTASE; MONOCLONAL-ANTIBODIES; MURINE MELANOCYTES; ALBINO LOCUS; METAL-IONS; CDNA AB Two pigmentation related genes have recently been cloned which map to the brown (b) and albino (c) loci of mice; these loci influence the quality and quantity, respectively, of melanin produced by melanocytes. Both these gene products are biochemically similar and have extensive amino acid sequence similarity to each other and to lower forms of tyrosinase (EC 1.14.18.1), a copper binding enzyme responsible for melanin production. In order to characterize the catalytic activities of these molecules, we have synthesized peptides and prepared antibodies to them which specifically recognize the gene products in question. By use of immune affinity purification protocols, we have isolated the proteins encoded by the brown and albino loci and have determined that both have the catalytic functions ascribed to tyrosinase, i.e. hydroxylation of tyrosine to 3,4-dihydroxyphenylalanine (DOPA) and the oxidation of DOPA to DOPAquinone. These are the critical reactions to melanogenesis since melanin pigment can be spontaneously produced from those products. The specific activity of the albino locus encoded product is considerably higher than that of the protein encoded by the brown locus, although the latter protein is present in higher quantity in melanocytes than is the protein encoded by the albino locus. These results are surprising since it was anticipated that tyrosinase was the product of single gene locus, and suggest that regulation of melanogenesis in mammals is controlled at the enzymatic level by several different gene products. C1 NIH,CELL BIOL LAB,BLDG 37,RM 1B22,BETHESDA,MD 20892. NR 67 TC 231 Z9 231 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 15 PY 1991 VL 266 IS 2 BP 1147 EP 1156 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET177 UT WOS:A1991ET17700074 PM 1898730 ER PT J AU BRENOWITZ, M MANDAL, N PICKAR, A JAMISON, E ADHYA, S AF BRENOWITZ, M MANDAL, N PICKAR, A JAMISON, E ADHYA, S TI DNA-BINDING PROPERTIES OF A LAC REPRESSOR MUTANT INCAPABLE OF FORMING TETRAMERS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID FOOTPRINT TITRATION; OPERATOR; GAL; RECOGNITION; RESOLUTION; COMPLEXES; SITES AB The interaction of proteins bound to sites widely separated on the genome is a recurrent motif in both prokaryotic and eukaryotic regulatory systems. Lac repressor mediates the formation of "DNA loops" by the simultaneous interaction of a single protein tetramer with two DNA-binding sites. The DNA-binding properties of a Lac repressor mutant (LacI(adi)) deficient in the association of protein dimers to tetramers was investigated. The results of quantitative footprint and gel mobility-shift titrations suggest that the wild-type Lac repressor (LacI+) binds cooperatively to two operator sites separated by 11 helical turns on a linear DNA restriction fragment by the formation of a "looped complex." LacI(adi) binds to this two-site operator non-cooperatively and without formation of a looped complex. These results demonstrate that the dimer-tetramer association of LacI+ is directly responsible for its cooperative binding and its ability to mediate formation of a looped complex. The I(adi) mutation disrupts the monomer-dimer as well as eliminating the dimer-tetramer association equilibria while the DNA binding affinity of LacI(adi) to a single site is unchanged relative to the wild-type protein. These results suggest that DNA binding and dimer-tetramer association are functionally unlinked. The similarity of the DNA-binding properties of LacI(adi) and Gal repressor, a protein believed to function by mediating the formation of a looped complex, are discussed. C1 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT BIOCHEM,BRONX,NY 10461. NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. FU NIGMS NIH HHS [GM39929] NR 35 TC 61 Z9 61 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 15 PY 1991 VL 266 IS 2 BP 1281 EP 1288 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET177 UT WOS:A1991ET17700092 PM 1985947 ER PT J AU GUY, R FOOPHILIPS, M SHARROW, SO HODES, RJ AF GUY, R FOOPHILIPS, M SHARROW, SO HODES, RJ TI SUBPOPULATIONS OF FETAL THYMOCYTES DEFINED BY EXPRESSION OF T-CELL RECEPTOR CD3 AND IL-2 RECEPTOR - CD3 AND IL-2 RECEPTOR ALPHA-CHAIN ARE EXPRESSED ON RECIPROCAL CELL-POPULATIONS SO JOURNAL OF IMMUNOLOGY LA English DT Article ID INTERLEUKIN-2 RECEPTOR; MONOCLONAL-ANTIBODY; IMMATURE THYMOCYTES; PROGENITOR CELLS; DIFFERENTIATION; IDENTIFICATION; INVITRO; PROLIFERATION; SUBSETS; GROWTH AB The expression of the TCR/CD3 complex and the IL-2R-alpha chain (p55) on fetal thymocytes has been analyzed by flow cytometry (FCM). Two-parameter immunofluorescence identified three subpopulations which were respectively IL-2R-alpha-/CD3+, IL-2R-alpha+/CD3-, or IL-2R-alpha-/CD3-; no detectable population of IL-2R-alpha+/CD3+ cells was found in unstimulated fetal thymocytes. Fractionation by "panning" and by sterile flow cytometric separation was used to characterize the functional responsiveness of these three subpopulations to a variety of stimuli. All three populations proliferated in response to PMA + ionomycin + rIL-2. In contrast, stimulation with anti-CD3 + IL-2 induced proliferation in IL-2R-alpha-/CD3+ and IL-2R-alpha-/CD3- but not in IL-2R-alpha+/CD3- thymocytes. IL-2R-alpha- cells, including sorted IL-2R-alpha-/CD3- thymocytes, underwent a phenotypic change in response to in vitro stimulation with anti-CD3 + IL-2, resulting in the appearance of an IL-2R-alpha+/CD3+ population that was not detected in freshly isolated thymocytes. The ability of fractionated fetal thymocytes to produce lymphokine in response to PMA + ionomycin was also evaluated. Only the IL-2R-alpha-/CD3- fraction generated detectable IL-2. These findings demonstrate for the first time that CD3 and IL-2R-alpha are expressed in a mutually exclusive fashion in fetal thymocytes and define three subpopulations of thymocytes that differ significantly in their proliferative and differentiative responses to TCR-mediated, IL-2R-mediated, and pharmacologic stimulation. C1 NCI,EXPTL IMMUNOL BRANCH,BLDG 10,10 ROOM 4B17,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 31 TC 8 Z9 8 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD JAN 15 PY 1991 VL 146 IS 2 BP 418 EP 424 PG 7 WC Immunology SC Immunology GA EU505 UT WOS:A1991EU50500003 PM 1824773 ER PT J AU HOFFMAN, T BRANDO, C LIZZIO, EF LEE, YL HANSEN, M TRIPATHI, AK TAPLITS, M PURI, J BONVINI, E ABRAHAMSEN, TG CARTER, CS REID, GK EVANS, J AF HOFFMAN, T BRANDO, C LIZZIO, EF LEE, YL HANSEN, M TRIPATHI, AK TAPLITS, M PURI, J BONVINI, E ABRAHAMSEN, TG CARTER, CS REID, GK EVANS, J TI CALCIUM-DEPENDENT EICOSANOID METABOLISM BY CONCANAVALIN A-STIMULATED HUMAN MONOCYTES INVITRO - SYNERGISM WITH PHORBOL ESTER INDICATES SEPARATE REGULATION OF LEUKOTRIENE-B4 SYNTHESIS AND RELEASE SO JOURNAL OF IMMUNOLOGY LA English DT Article ID SUPEROXIDE ANION GENERATION; ARACHIDONIC-ACID RELEASE; 5-LIPOXYGENASE PATHWAY; MOBILIZING LIGANDS; HUMAN-NEUTROPHILS; KINASE-C; ACTIVATION; IONOPHORE-A23187; MACROPHAGES; MEMBRANE AB Human monocytes obtained by counter-current centrifugal elutriation released arachidonic acid when challenged in vitro with Con A, as well as with other soluble (PMA or ionomycin) or particulate stimuli (serum-treated zymosan). Cyclo-oxygenase metabolites were the principal eicosanoids detected in the supernatants of Con A-stimulated, [H-3]arachidonate-labeled monocytes. 5-Lipoxygenase (5-LO) products, such as leukotriene B4 (LTB4), were conspiciously absent. Release of arachidonate and its metabolites in response to Con A was dependent on the presence of extracellular Ca2+, but not Mg2+. In contrast to serum-treated zymosan challenge, which resulted in increased inositol trisphosphate and LTB4 release, Con A-induced inositol phospholipid hydrolysis in monocytes was limited to phosphadidylinositol or phosphatidylinositol monosphosphate. Despite an inability to augment LTB4 release, Con A or PMA induced a loss of 5-lipoxygenase from a cytosolic compartment that was similar to that achieved with a calcium ionophore (ionomycin), a potent stimulus for LTB4 generation. When cell-associated LTB4 was evaluated, evidence for increased LTB4 production was obtained in response to either stimulus (PMA > Con A). In combination, however, PMA and Con A treatment resulted in monocyte LTB4 release comparable with that observed with the calcium ionophore or STZ. LTB4 release in response to all stimuli tested was inhibited by MK-886, a drug that binds to 5-lipoxygenase-activating protein. These results indicate the following: 1) Phospholipase A2 activation and attendant arachidonic acid release induced by agents that increase intracellular Ca2+ and/or generate diacylglycerol results in increased synthesis and release of PG and increased synthesis of leukotrienes, but not necessarily leukotriene release. 2) 5-LO translocation which may occur independently of increased intracellular Ca2+, may be necessary for LTB4 generation but is insufficient for its release. 3) 5-Lipoxygenase-activating protein activity is necessary for 5-LO activation and LTB4 release in response to all stimuli investigated here. 4) Phorbol ester, an activator of protein kinase C, may synergize with agents such as Con A (which by themselves induce a minimal intracellular Ca2+ rise), so as to result in the release of LTB4. Thus, Con A may represent a class of surface receptor-aggregating agents that initiates inflammatory changes or immunomodulation associated with liberation of PG and might predispose to release of other inflammatory mediators, such as leukotrienes, in the presence of additional signals, including protein kinase activation. C1 NCI,PEDIAT ONCOL BRANCH,DIV CANC THERAPY,BETHESDA,MD 20892. NIH,CTR CLIN,DEPT TRANSFUS MED,BETHESDA,MD 20892. MERCK FROSST CTR THERAPEUT RES,POINTE CLAIRE H9R 4P8,QUEBEC,CANADA. US FDA,CTR BIOL EVALUAT & RES,DIV HEMATOL,CELL BIOL LAB,BETHESDA,MD 20892. NR 24 TC 27 Z9 27 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD JAN 15 PY 1991 VL 146 IS 2 BP 692 EP 700 PG 9 WC Immunology SC Immunology GA EU505 UT WOS:A1991EU50500042 PM 1846160 ER PT J AU KATONA, IM OHURA, K ALLEN, JB WAHL, LM CHENOWETH, DE WAHL, SM AF KATONA, IM OHURA, K ALLEN, JB WAHL, LM CHENOWETH, DE WAHL, SM TI MODULATION OF MONOCYTE CHEMOTACTIC FUNCTION IN INFLAMMATORY LESIONS - ROLE OF INFLAMMATORY MEDIATORS SO JOURNAL OF IMMUNOLOGY LA English DT Article ID TUMOR NECROSIS FACTOR; CENTRIFUGAL ELUTRIATION CCE; PERIPHERAL-BLOOD MONOCYTES; MONONUCLEAR CELL SUBSETS; RHEUMATOID-ARTHRITIS; ENRICHED FRACTIONS; LIGAND RECEPTORS; INTERFERON-GAMMA; SYNOVIAL-FLUID; INTERLEUKIN-1 AB Monocyte recruitment and accumulation in the synovial tissue is pivotal in the evolution of rheumatoid arthritis (RA). In the present study we examined the chemotactic potential of monocytes obtained from synovial fluid (SF) of patients with RA. Functionally, SF monocytes exhibited greatly diminished chemotactic activity to C5a compared with monocytes from the peripheral blood. In contrast, their chemotactic responsiveness to the synthetic peptide, FMLP, was nearly normal. To define a mechanism for this differential chemotactic dysfunction, cell-surface receptors for C5a (C5aR) and FMLP (FMLP-R) were evaluated. Whereas FMLP-R expression was similar on both blood and inflamatory monocytes, C5aR expression was markedly reduced on SF cells. Because decreased C5a binding in certain RA SF samples could not be attributed to free C5a, known or suspected components of inflammatory SF were evaluated for their ability to modulate chemotactic ligand receptors. Bacterial products including LPS and streptococcal cell walls, which are potent monocyte activators, down-regulated C5aR without affecting FMLP-R. Moreover, the cytokines IFN-gamma and granulocyte-macrophage-CSF selectively decreased C5aR in parallel with decreased in vitro chemotactic activity to C5a. Thus, these data indicate that 1) synovial effusions may contain C5a and/or inflammatory mediators that modulate phenotypic and functional changes in monocytes, 2) chemotactic ligand receptors are independently regulated in inflammatory lesions, and 3) decreased C5aR expression and chemotactic potential likely provide a mechanism whereby monocyte-macrophages persist within the inflamed synovium. C1 UNIFORMED SERV UNIV HLTH SCI,F EDWARD HEBERT SCH MED,DEPT PEDIAT & MED,RHEUMATOL SECT,BETHESDA,MD 20814. NIDR,IMMUNOL LAB,CELLULAR IMMUNOL SECT,BETHESDA,MD 20892. BAXTER HLTH CARE,SANTA ANA,CA 92705. NR 44 TC 20 Z9 21 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD JAN 15 PY 1991 VL 146 IS 2 BP 708 EP 714 PG 7 WC Immunology SC Immunology GA EU505 UT WOS:A1991EU50500044 PM 1898961 ER PT J AU GITTERMAN, M WEISS, GH AF GITTERMAN, M WEISS, GH TI SOME COMMENTS ON APPROXIMATIONS TO THE MASTER EQUATION SO PHYSICA A LA English DT Article ID NUCLEATION; GROWTH AB A common strategy for approximating a master equation is to replace it by a diffusion-like equation. Many methods for deriving the form of such an equation have been suggested in the literature. We compare two of these in the light of an example in which the master equation can be solved exactly. One of these is the van Kampen OMEGA-expansion, which generally does not give a useful approximation to the equilibrium solution, and the second is a technique which preserves the noise-free dynamics and gives the correct equilibrium solution. It is shown that the second moment calculated in the latter approximation is not an accurate one at short times. The difficulty is the restriction of the approximating equation to the diffusion form. C1 NIH,BETHESDA,MD 20892. RP GITTERMAN, M (reprint author), BAR ILAN UNIV,DEPT PHYS,IL-52100 RAMAT GAN,ISRAEL. NR 13 TC 6 Z9 6 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4371 J9 PHYSICA A JI Physica A PD JAN 15 PY 1991 VL 170 IS 3 BP 503 EP 510 DI 10.1016/0378-4371(91)90004-V PG 8 WC Physics, Multidisciplinary SC Physics GA EX973 UT WOS:A1991EX97300004 ER PT J AU SCHWARZER, S LEE, J HAVLIN, S STANLEY, HE MEAKIN, P AF SCHWARZER, S LEE, J HAVLIN, S STANLEY, HE MEAKIN, P TI DISTRIBUTION OF GROWTH PROBABILITIES FOR OFF-LATTICE DIFFUSION-LIMITED AGGREGATION SO PHYSICAL REVIEW A LA English DT Note ID PHASE-TRANSITION AB We study the distribution n(a,M) of growth probabilities {p(i)} for off-lattice diffusion-limited aggregation (DLA) for cluster sizes up to mass M = 20 000, where a-i = -logp(i)/logM. We find that for large a, logn(a,M) proportional-to - a-gamma/log-delta-M, with gamma = 2 +/- 0.3 and delta = 1.3 +/- 0.3. One consequence of this form is that the minimum growth probability p(min)(M) obeys the asymptotic relation logp(min)(M) similar-to -(logM)(gamma+1+delta)/gamma. We find evidence for the existence of a well-defined crossover value a* such that only the rare configurations of DLA contribute to n(a,M) for a > a*, while both rare and typical DLA configurations contribute for a < a*. C1 BOSTON UNIV,DEPT PHYS,BOSTON,MA 02215. UNIV HAMBURG,INST THEORET PHYS,W-2000 HAMBURG 36,GERMANY. NIH,DIV COMP RES & TECHNOL,BETHESDA,MD 20892. DUPONT CO,CENT RES & DEV DEPT,WILMINGTON,DE 19880. RP SCHWARZER, S (reprint author), BOSTON UNIV,CTR POLYMER STUDIES,BOSTON,MA 02215, USA. NR 26 TC 25 Z9 25 U1 0 U2 1 PU AMERICAN PHYSICAL SOC PI COLLEGE PK PA ONE PHYSICS ELLIPSE, COLLEGE PK, MD 20740-3844 USA SN 1050-2947 J9 PHYS REV A JI Phys. Rev. A PD JAN 15 PY 1991 VL 43 IS 2 BP 1134 EP 1137 DI 10.1103/PhysRevA.43.1134 PG 4 WC Optics; Physics, Atomic, Molecular & Chemical SC Optics; Physics GA EU856 UT WOS:A1991EU85600060 ER PT J AU HORNE, MK AF HORNE, MK TI HEPARIN BINDS NORMALLY TO PLATELETS DIGESTED WITH STREPTOMYCES-GRISEUS PROTEASE SO THROMBOSIS RESEARCH LA English DT Note DE HEPARIN; PLATELET MEMBRANE GLYCOPROTEINS ID PROTEINS; GLYCOSAMINOGLYCANS; GLYCOPROTEINS; MEMBRANE RP HORNE, MK (reprint author), NIH,WARREN G MAGNUSON CLIN CTR,DEPT CLIN PATHOL,HEMATOL SERV,BETHESDA,MD, USA. NR 15 TC 3 Z9 3 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0049-3848 J9 THROMB RES JI Thromb. Res. PD JAN 15 PY 1991 VL 61 IS 2 BP 155 EP 158 DI 10.1016/0049-3848(91)90242-O PG 4 WC Hematology; Peripheral Vascular Disease SC Hematology; Cardiovascular System & Cardiology GA EV179 UT WOS:A1991EV17900007 PM 1902330 ER PT J AU VOIGT, MM MCCUNE, SK KANTERMAN, RY FELDER, CC AF VOIGT, MM MCCUNE, SK KANTERMAN, RY FELDER, CC TI THE RAT ALPHA-2-C4 ADRENERGIC-RECEPTOR GENE ENCODES A NOVEL PHARMACOLOGICAL SUBTYPE SO FEBS LETTERS LA English DT Article DE ALPHA-2-ADRENOCEPTOR; GENE EXPRESSION; GENE COUPLING; CAMP INHIBITION ID ALPHA-2-ADRENERGIC RECEPTOR; CELLS; EXPRESSION; INHIBITION; CLONING; BINDING AB A rat gene and brain cDNA (pA2d) encoding the homologue of the human alpha-C4 adrenergic receptor subtype were isolated and characterized. RNA blots indicate that this gene is expressed in brain, heart and kidney but not in lung, liver or pancreas. Yohimbine, WB-4101 and prasozin all exhibited high affinity for this receptor in binding studies. Clonidine was more potent and efficacious than norepinephrine in inhibiting forskolin-stimulated cAMP production in CHO cells expressing pA2d. Together, these data suggest that the alpha-2-C4 gene product defines a previously undescribed pharmacological subtype of alpha-2-adrenergic receptor. C1 NICHHD,DEV NEUROBIOL LAB,BETHESDA,MD 20817. HOWARD HUGHES MED INST,NIH RES SCHOLARS PROGRAM,BETHESDA,MD 20892. NINCDS,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 19 TC 67 Z9 68 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD JAN 14 PY 1991 VL 278 IS 1 BP 45 EP 50 DI 10.1016/0014-5793(91)80080-M PG 6 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA EY740 UT WOS:A1991EY74000012 PM 1704314 ER PT J AU FINE, A AULT, B RAPOPORT, SI AF FINE, A AULT, B RAPOPORT, SI TI MOUSE TRISOMY-16 NEURONS, A MODEL OF HUMAN TRISOMY-21 (DOWN-SYNDROME), CAN BE MAINTAINED BY INTRACEREBRAL TRANSPLANTATION SO NEUROSCIENCE LETTERS LA English DT Article DE TRISOMY-16; TRISOMY-21; DOWN SYNDROME; NEURAL TRANSPLANTATION; ALZHEIMERS DISEASE; BRAIN ID ROOT GANGLION NEURONS; HUMAN CHROMOSOME-21; ANOMALIES; FETUS AB The trisomy 16 mouse is considered to be a model of human trisomy 21 (Down syndrome) due to genetic homology between parts of human chromosome 21 and mouse chromosome 16. Additionally, and because older Down syndrome individuals develop neuropathology indistinguishable from that of Alzheimer's disease, trisomy 16 tissue may provide a model of some pathological processes occurring in Alzheimer's disease. However, trisomy 16 fetuses die in utero or shortly after birth, preventing exploitation of this model. We therefore sought to examine trisomy 16 brain tissue over an extended period of time. We report that neural transplantation to normal hosts allows the maintenance of cortical and hippocampal neurons for at least 8 months. We report that neural transplantation to normal hosts allows the maintenance of cortical and hippocampal neurons for at least 8 months, thus providing a model in which to examine pathological processes related to Down syndrome, and perhaps to Alzheimer's disease. C1 NIA,NEUROSCI LAB,BETHESDA,MD 20892. RP FINE, A (reprint author), DALHOUSIE UNIV,DEPT PHYSIOL & BIOPHYS,HALIFAX B3H 4H7,NS,CANADA. NR 20 TC 12 Z9 12 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD JAN 14 PY 1991 VL 122 IS 1 BP 4 EP 8 DI 10.1016/0304-3940(91)90179-W PG 5 WC Neurosciences SC Neurosciences & Neurology GA EV966 UT WOS:A1991EV96600002 PM 1711657 ER PT J AU LOVINGER, DM AF LOVINGER, DM TI ETHANOL POTENTIATION OF 5-HT3 RECEPTOR-MEDIATED ION CURRENT IN NCB-20 NEUROBLASTOMA-CELLS SO NEUROSCIENCE LETTERS LA English DT Article DE ALCOHOL; INTOXICATION; SEROTONIN; PATCH-CLAMP; NEUROBLASTOMA; ION CHANNEL ID RELEASE; CHANNELS; LINES; RATS AB The effect of acute ethanol (EtOH) exposure on 5-HT3 receptor-mediated ion current was examined in whole-cell patch-clamp recordings from NCB-20 neuroblastoma cells. The physiologic and pharmacologic properties of 5-HT-activated ion current in NCB-20 cells indicated that it was mediated by 5-HT3 receptors. EtOH potentiated 5-HT3 receptor-mediated current in a concentration-dependent manner at concentrations (25-100 mM) which are achieved during EtOH intoxication in vivo. RP LOVINGER, DM (reprint author), NIAAA,LPPS,ELECTROPHYSIOL SECT,SYNAPT PHARMACOL UNIT,ROCKVILLE,MD 20852, USA. NR 20 TC 89 Z9 90 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD JAN 14 PY 1991 VL 122 IS 1 BP 57 EP 60 DI 10.1016/0304-3940(91)90192-V PG 4 WC Neurosciences SC Neurosciences & Neurology GA EV966 UT WOS:A1991EV96600015 PM 1711659 ER PT J AU ELLENBERG, SS AF ELLENBERG, SS TI SURROGATE END-POINTS IN CLINICAL-TRIALS - GETTING CLOSER TO IDENTIFYING MARKERS FOR SURVIVAL IN AIDS SO BMJ-BRITISH MEDICAL JOURNAL LA English DT Editorial Material ID ENDPOINTS RP ELLENBERG, SS (reprint author), NIAID, DIV AIDS, BIOSTAT RES BRANCH, BETHESDA, MD 20892 USA. NR 9 TC 20 Z9 20 U1 0 U2 1 PU BMJ PUBLISHING GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 1756-1833 J9 BMJ-BRIT MED J JI BMJ-British Medical Journal PD JAN 12 PY 1991 VL 302 IS 6768 BP 63 EP 64 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA ER951 UT WOS:A1991ER95100003 PM 1671650 ER PT J AU PONCE, C PONCE, E MORRISON, A CRUZ, A KREUTZER, R MCMAHONPRATT, D NEVA, F AF PONCE, C PONCE, E MORRISON, A CRUZ, A KREUTZER, R MCMAHONPRATT, D NEVA, F TI LEISHMANIA-DONOVANI-CHAGASI - NEW CLINICAL VARIANT OF CUTANEOUS LEISHMANIASIS IN HONDURAS SO LANCET LA English DT Article ID INFANTUM SENSU-LATO; MONOCLONAL-ANTIBODIES; IDENTIFICATION AB During surveillance for endemic visceral leishmaniasis on an island off the Pacific coast of Honduras, an unusual form of cutaneous leishmaniasis was encountered. By clinical and laboratory criteria, 17 cases were identified over 5 months; children aged 4 to 15 years were primarily affected. Lesions were generally few in number, small, always papular, and non-ulcerative, even when present for several years. Patients with skin lesions seemed otherwise healthy and were well nourished. Montenegro skin tests with Leishmania mexicana and L major antigens were positive in 10 of 17 patients tested, and lesions from 9 patients were positive by culture. Since the summer of 1988, cases of atypical cutaneous leishmaniasis continue to occur on the island (8) as well as on the mainland of southern Honduras (23). A total of 9 parasite isolates from skin lesions, 4 from bone marrow of patients with kala-azar, and 2 from sandflies were identified as L donovani chagasi and were indistinguishable from one another by isoenzyme analysis. C1 NIAID,PARASIT DIS LAB,BLDG 4,ROOM 126,BETHESDA,MD 20892. YALE UNIV,DEPT EPIDEMIOL & PUBL HLTH,NEW HAVEN,CT 06520. YOUNGSTOWN STATE UNIV,DEPT BIOL,YOUNGSTOWN,OH 44555. HOSP ESCUELA TEGUCIGALPA,TEGUCIGALPA,HONDURAS. MINIST HLTH,CENT LAB,TEGUCIGAL,HONDURAS. NR 18 TC 66 Z9 68 U1 0 U2 1 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD JAN 12 PY 1991 VL 337 IS 8733 BP 67 EP 70 DI 10.1016/0140-6736(91)90734-7 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA ER477 UT WOS:A1991ER47700002 PM 1670724 ER PT J AU RAVUSSIN, E AF RAVUSSIN, E TI SAFETY OF GROWTH-HORMONE SO LANCET LA English DT Letter ID ENERGY-EXPENDITURE RP RAVUSSIN, E (reprint author), NIH,CLIN DIABET & NUTR SECT,PHOENIX,AZ 85016, USA. NR 6 TC 0 Z9 0 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD JAN 12 PY 1991 VL 337 IS 8733 BP 109 EP 109 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA ER477 UT WOS:A1991ER47700028 ER PT J AU MASANA, MI BITRAN, JA HSIAO, JK MEFFORD, IN POTTER, WZ AF MASANA, MI BITRAN, JA HSIAO, JK MEFFORD, IN POTTER, WZ TI LITHIUM EFFECTS ON NORADRENERGIC-LINKED ADENYLATE-CYCLASE ACTIVITY IN INTACT RAT-BRAIN - AN INVIVO MICRODIALYSIS STUDY SO BRAIN RESEARCH LA English DT Note DE LITHIUM; CYCLIC ADENOSINE MONOPHOSPHATE; BETA-RECEPTOR; INVIVO MICRODIALYSIS; NOREPINEPHRINE ID CYCLIC-AMP ACCUMULATION; CEREBRAL-CORTEX; ELECTROCONVULSIVE SHOCK; CORTICAL SLICES; INHIBITION; INVITRO; MEMBRANES; RECEPTOR; RELEASE; EXVIVO AB The effects of chronic lithium treatment on adenylate cyclase activity in intact rat brain were examined using in vivo microdialysis. Basal extracellular cyclic adenosine monophosphate (AMP) increased in a dose-dependent manner after norepinephrine was added to the perfusate. Chronic lithium treatment increased basal brain extracellular fluid cyclic AMP levels, while decreasing the magnitude of the cyclic AMP response to stimulation with 100-mu-M norepinephrine. RP MASANA, MI (reprint author), NIMH,CLIN NEUROSCI BRANCH,CLIN PHARMACOL SECT,BLDG 10,ROOM 2D46,BETHESDA,MD 20892, USA. NR 21 TC 42 Z9 43 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD JAN 11 PY 1991 VL 538 IS 2 BP 333 EP 336 DI 10.1016/0006-8993(91)90450-A PG 4 WC Neurosciences SC Neurosciences & Neurology GA ET860 UT WOS:A1991ET86000024 PM 1849439 ER PT J AU ROBEY, EA FOWLKES, BJ GORDON, JW KIOUSSIS, D VONBOEHMER, H RAMSDELL, F AXEL, R AF ROBEY, EA FOWLKES, BJ GORDON, JW KIOUSSIS, D VONBOEHMER, H RAMSDELL, F AXEL, R TI THYMIC SELECTION IN CD8 TRANSGENIC MICE SUPPORTS AN INSTRUCTIVE MODEL FOR COMMITMENT TO A CD4 OR CD8 LINEAGE SO CELL LA English DT Article ID T-CELL RECEPTOR; ANTIGEN RECEPTOR; POSITIVE SELECTION; LYMPHOCYTES-T; GENE; MOLECULES; EXPRESSION; RECOGNITION; ACTIVATION; ENGAGEMENT AB Immature thymocytes, which coexpress CD4 and CD8, give rise to mature CD4+CD8- and CD4-CD8+ T cells. Only those T cells that recognize self-MHC are selected to mature, a process known as positive selection. The specificity of the T cell antigen receptor (TCR) for class I or class II MHC influences the commitment to a CD4 or CD8 lineage. This may occur by a directed mechanism or by stochastic commitment followed by a selection step that allows only CD8+, class I-specific and CD4+, class II-specific cells to survive. We have generated a mouse line expressing a CD8 transgene under the control of the T cell-specific CD2 regulatory sequences. Although constitutive CD8 expression does not affect thymic selection of CD4+ cells, selection of a class I-specific TCR in the CD8 subset is substantially improved. This outcome is consistent with a model for positive selection in which selection occurs at a developmental stage in which both CD4 and CD8 are expressed, and positive selection by class I MHC generates an instructive signal that directs differentiation to a CD8 lineage. C1 COLUMBIA UNIV,HOWARD HUGHES MED INST,NEW YORK,NY 10032. NIAID,CELLULAR & MOLEC IMMUNOL LAB,BETHESDA,MD 20892. CUNY MT SINAI SCH MED,DEPT OBSTET GYNECOL & REPROD SCI,NEW YORK,NY 10029. CUNY MT SINAI SCH MED,DEPT BIOCHEM,NEW YORK,NY 10029. NATL INST MED RES,GENE STRUCT & EXPRESS LAB,LONDON NW7 1AA,ENGLAND. BASEL INST IMMUNOL,CH-4005 BASEL,SWITZERLAND. RP ROBEY, EA (reprint author), COLUMBIA UNIV,DEPT BIOCHEM & MOLEC BIOPHYS,NEW YORK,NY 10032, USA. NR 41 TC 213 Z9 213 U1 0 U2 3 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD JAN 11 PY 1991 VL 64 IS 1 BP 99 EP 107 DI 10.1016/0092-8674(91)90212-H PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA ET175 UT WOS:A1991ET17500012 PM 1898873 ER PT J AU HAYNES, SR JOHNSON, D RAYCHAUDHURI, G BEYER, AL AF HAYNES, SR JOHNSON, D RAYCHAUDHURI, G BEYER, AL TI THE DROSOPHILA HRB87F GENE ENCODES A NEW MEMBER OF THE A-HNRNP AND B-HNRNP PROTEIN GROUP SO NUCLEIC ACIDS RESEARCH LA English DT Article ID HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; RNA-BINDING PROTEINS; HELIX-DESTABILIZING PROTEIN; MESSENGER-RNA; CDNA CLONING; CORE PROTEINS; NUCLEOTIDE-SEQUENCE; CONSENSUS SEQUENCE; SNRNP PROTEIN; U1 SNRNA AB Nascent premessenger RNA transcripts are packaged into heterogeneous nuclear ribonucleoprotein (hnRNP) complexes containing specific nuclear proteins, the hnRNP proteins. The A and B group proteins constitute a major class of small basic proteins found in mammalian hnRNP complexes. We have previously characterized the Drosophila melanogaster Hrb98DE gene, which is alternatively spliced to encode four protein isoforms closely related to the A and B proteins. We report here that the Drosophila genome contains a family of genes related to the Hrb98DE gene. One member of the family, Hrb87F, is very homologous to Hrb98DE in both sequence and structure. The Hrb87F transcripts (1.7 and 2.2 kb) utilize two alternative polyadenylation sites, are abundant in ovaries and early embryos, and are present in lesser amounts throughout development. In one wildtype strain of Drosophila there is a naturally-occurring polymorphism in this gene due to the insertion of a 412 transposable element in the 3' untranslated region. The larger transcript is not produced in these files and thus is not required for viability. Sequence identities among the Drosophila Hrb proteins and the vertebrate A and B hnRNP proteins suggest that these proteins may form a distinct subfamily within the larger family of related RNA binding proteins. C1 UNIV VIRGINIA,SCH MED,DEPT MICROBIOL,CHARLOTTESVILLE,VA 22908. GEORGE WASHINGTON UNIV,DEPT BIOL SCI,WASHINGTON,DC 20052. RP HAYNES, SR (reprint author), NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892, USA. FU NIGMS NIH HHS [GM39271] NR 51 TC 55 Z9 56 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 11 PY 1991 VL 19 IS 1 BP 25 EP 31 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU151 UT WOS:A1991EU15100005 PM 1849257 ER PT J AU GRAESSMANN, M MICHAELS, G BERG, B GRAESSMANN, A AF GRAESSMANN, M MICHAELS, G BERG, B GRAESSMANN, A TI INHIBITION OF SV40 GENE-EXPRESSION BY MICROINJECTED SMALL ANTISENSE RNA AND DNA-MOLECULES SO NUCLEIC ACIDS RESEARCH LA English DT Article ID ANTI-SENSE RNA; SIMIAN VIRUS-40 ENHANCER; MESSENGER-RNA; SECONDARY STRUCTURE; CELLS; PROMOTER; INVITRO; TRANSFORMATION; DICTYOSTELIUM; PHENOCOPIES AB We tested the impact of antisense RNA and DNA molecules on SV40 gene expression by microinjection into TC7 cells. Short antisense stretches, complementary to either hairpin or loop structures on the T antigen mRNA, inhibited T antigen synthesis. In contrast, full-length antisense RNA and DNA molecules did not effect T antigen synthesis. C1 FREE UNIV BERLIN,INST MOLEK BIOL & BIOCHEM,ARNIMALLEE 22,BERLIN 33,GERMANY. NIH,BETHESDA,MD 20892. NR 43 TC 19 Z9 19 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 11 PY 1991 VL 19 IS 1 BP 53 EP 59 DI 10.1093/nar/19.1.53 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU151 UT WOS:A1991EU15100008 PM 1849259 ER PT J AU POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR AF POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR TI TETRANUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN AROMATASE CYTOCHROME-P-450 GENE (CYP19) SO NUCLEIC ACIDS RESEARCH LA English DT Note RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 4 TC 106 Z9 107 U1 0 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 11 PY 1991 VL 19 IS 1 BP 195 EP 195 DI 10.1093/nar/19.1.195 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU151 UT WOS:A1991EU15100040 PM 2011509 ER PT J AU HSIEH, WT BARRICK, JL HOEHE, MR GERSHON, ES AF HSIEH, WT BARRICK, JL HOEHE, MR GERSHON, ES TI A NOTI RFLP IN THE HUMAN ALPHA2-C4 ADRENERGIC-RECEPTOR LOCUS (ADRA2RL2) DETECTED BY PFGE SO NUCLEIC ACIDS RESEARCH LA English DT Note RP HSIEH, WT (reprint author), NIMH,ADAMHA,CLIN NEUROGENET BRANCH,BLDG 10,RM 3N218,BETHESDA,MD 20892, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 11 PY 1991 VL 19 IS 1 BP 198 EP 198 DI 10.1093/nar/19.1.198 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU151 UT WOS:A1991EU15100046 PM 1672746 ER PT J AU DEAN, M STEWART, C PERRY, A STAUFFER, D OTTERUD, B WHITE, R LEPPERT, M AF DEAN, M STEWART, C PERRY, A STAUFFER, D OTTERUD, B WHITE, R LEPPERT, M TI D7S448 DETECTS A HINDIII POLYMORPHISM LOCATED IN THE CENTROMERE REGION OF CHROMOSOME-7 SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 UNIV UTAH,DEPT HUMAN GENET,SALT LAKE CITY,UT 84132. UNIV UTAH,HOWARD HUGHES MED INST,SALT LAKE CITY,UT 84132. RP DEAN, M (reprint author), FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC DYNCORP,FREDERICK,MD 21702, USA. RI Dean, Michael/G-8172-2012 OI Dean, Michael/0000-0003-2234-0631 FU NCI NIH HHS [N01-CO-74102] NR 1 TC 4 Z9 4 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 11 PY 1991 VL 19 IS 1 BP 200 EP 200 DI 10.1093/nar/19.1.200-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU151 UT WOS:A1991EU15100051 PM 1672750 ER PT J AU TUOMANEN, E LINDQUIST, S SANDE, S GALLENI, M LIGHT, K GAGE, D NORMARK, S AF TUOMANEN, E LINDQUIST, S SANDE, S GALLENI, M LIGHT, K GAGE, D NORMARK, S TI COORDINATE REGULATION OF BETA-LACTAMASE INDUCTION AND PEPTIDOGLYCAN COMPOSITION BY THE AMP OPERON SO SCIENCE LA English DT Article ID PENICILLIN-BINDING PROTEINS; ESCHERICHIA-COLI; MUREIN; DELETION; GENE AB The amp operon, which is located on the Escherichia coli chromosome, modulates the induction of plasmid-borne beta-lactamase genes by extracellular beta-lactam antibiotics. This suggests that the gene products AmpD and AmpE may function in the transduction of external signals. Beta-Lactam antibiotics are analogs of cell wall components that can be released during cell wall morphogenesis of enterobacteria. The amp operon was studied to determine its importance in signal transduction during cell wall morphogenesis. The peptidoglycan compositions of amp mutants were determined by high-performance liquid chromotography and fast atom bombardment mass spectrometry. When a chromosomal or plasmid-borne copy of ampD was present, the amount of pentapeptide-containing muropeptides in the cell wall increased upon addition of the cell wall constituent diaminopimelic acid to the growth medium. These results suggest that beta-lactamase induction and modulation of the composition of the cell wall share elements of a regulatory circuit that involves AmpD. Escherichia coli requires AmpD to respond to extracellular signaling amino acids, such as diaminopimelic acid, and this signal transduction system may regulate peptidoglycan composition in response to cell wall turnover products. C1 UMEA UNIV,DEPT MICROBIOL,S-90187 UMEA,SWEDEN. WASHINGTON UNIV,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63110. MICHIGAN STATE UNIV,NIH,MASS SPECT FACIL,DEPT BIOCHEM,E LANSING,MI 48824. RP TUOMANEN, E (reprint author), ROCKEFELLER UNIV,MICROBIOL LAB,NEW YORK,NY 10021, USA. OI Tuomanen, Elaine/0000-0003-0349-8716 FU NCRR NIH HHS [DRR00480]; NIAID NIH HHS [AI23459, AI27913] NR 17 TC 40 Z9 46 U1 0 U2 2 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD JAN 11 PY 1991 VL 251 IS 4990 BP 201 EP 204 DI 10.1126/science.1987637 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET173 UT WOS:A1991ET17300042 PM 1987637 ER PT J AU HULLIN, F BOSSANT, MJ SALEM, N AF HULLIN, F BOSSANT, MJ SALEM, N TI AMINOPHOSPHOLIPID MOLECULAR-SPECIES ASYMMETRY IN THE HUMAN ERYTHROCYTE PLASMA-MEMBRANE SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Article DE TRINITROBENZENESULFONIC ACID; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLSERINE; PHOSPHOLIPID MOLECULAR SPECIES; MEMBRANE LIPID ASYMMETRY ID FATTY-ACID COMPOSITION; RED-CELL MEMBRANE; ARACHIDONIC-ACID; SELECTIVE INCORPORATION; DOCOSAHEXAENOIC ACID; CHEMICAL PROBES; PHOSPHOLIPIDS; RAT; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLCHOLINE AB The transbilayer distribution of the molecular species of aminophospholipids in human red blood cell plasma membrane has been investigated using a covalent labelling technique. Separation and quantitative analysis of the molecular species of phosphatidylethanolamine (PE) and phosphatidylserine (PS) was performed using high-performance liquid chromatography with UV detection of the trinitrophenyl derivatives obtained after reaction with trinitrobenzenesulfonic acid (TNBS). When the molecular species distribution obtained with intact cells was compared to that of the whole membrane, a molecular species asymmetry was evident. This phenomenon was most clearly evident when the reaction was performed at low temperatures (0-degrees-C) and was obscured by the excessive labelling or probe permeation associated with higher temperatures or longer incubation times. The monoene species were enriched in the outer leaflet, they comprised about 30% of the PE species in this leaflet. The polyunsaturates were preferentially localized in the inner leaflet and this was true of the arachidonyl species in particular as they represented up to 35% of this pool. The w-3 polyunsaturated fatty acids displayed a preferential localization in the plasmalogen subclass in comparison to the diacyl fraction, i.e., they comprised about 58 of the former and 42% of of the latter subclass of cellular PE w-3 species. Data concerning the separation, identification and quantification of PS molecular species in human erythrocytes is also presented. The internal localization of the polyunsaturated species as well as the compartmentalization of the w-3 and w-6 pools will have metabolic, structural and physical implications for membrane function. C1 NIAAA,ADAMHA,DICBR,ANALYT CHEM SECT,CLIN STUDIES LAB,BLDG 10,RM 3C-102,BETHESDA,MD. NR 44 TC 32 Z9 33 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD JAN 9 PY 1991 VL 1061 IS 1 BP 15 EP 25 DI 10.1016/0005-2736(91)90263-8 PG 11 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EW159 UT WOS:A1991EW15900003 PM 1995054 ER PT J AU HORIO, M LOVELACE, E PASTAN, I GOTTESMAN, MM AF HORIO, M LOVELACE, E PASTAN, I GOTTESMAN, MM TI AGENTS WHICH REVERSE MULTIDRUG-RESISTANCE ARE INHIBITORS OF [H-3] VINBLASTINE TRANSPORT BY ISOLATED VESICLES SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Article DE P-GLYCOPROTEIN; L-LEUCINE TRANSPORT; ATP; VERAPAMIL; QUINIDINE; OSMOLARITY ID MULTIPLE-DRUG RESISTANCE; CALCIUM-CHANNEL BLOCKERS; CYTO-TOXICITY; HUMAN CANCER; CELLS; VINBLASTINE; GLYCOPROTEIN; VINCRISTINE; ADRIAMYCIN AB Resistance of human cancer cells to multiple cytotoxic hydrophobic agents (multidrug resistance) is due to overexpression of the MDR1 gene whose product is the ATP-dependent multidrug transporter, P-glycoprotein. We have previously reported that plasma membrane vesicles partially purified from multidrug-resistant human KB carcinoma cells, but not from drug-sensitive cells, accumulated [H-3]vinblastine in an ATP-dependent manner (Horio, M., Gottesman, M.M. and Pastan, I. (1988) Proc. Natl. Acad. Sci. USA 85, 3580-3584). Certain calcium-channel blockers, quinidine, and phenothiazines are able to overcome multidrug resistance in cultured cells. In this work, the effect of these reversing agents on ATP-dependent vinblastine (VBL) transport by vesicles from drug-resistant KB cells has been characterized. Azidopine was the most potent inhibitor of ATP-dependent VBL uptake tested (ID50: concentration of inhibitor such that the transport of vinblastine is inhibited by 50%, < 1-mu-M). Verapamil, quinidine, and the tiapamil analogue RO-11-2933 were potent but less effective inhibitors (ID50 < 5-mu-M). Diltiazem, nifedipine and trifluoperazine were even less effective. These agents had no effect on Na+-dependent and Na+-independent L-leucine uptake by the vesicles, indicating that the inhibition of ATP dependent VBL transport by these agents is not a non-specific effect, as might result from leaks in the vesicle membrane. Verapamil, quinidine, azidopine and trifluoperazine increased the apparent K(m) value of vinblastine transport, suggesting that these agents may be competitive inhibitors of vinblastine transport. C1 NCI,MOLEC BIOL LAB,BLDG 37,ROOM 2E18,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 12 TC 99 Z9 101 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD JAN 9 PY 1991 VL 1061 IS 1 BP 106 EP 110 DI 10.1016/0005-2736(91)90274-C PG 5 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EW159 UT WOS:A1991EW15900014 PM 1671642 ER PT J AU CLORE, GM KAY, LE BAX, A GRONENBORN, AM AF CLORE, GM KAY, LE BAX, A GRONENBORN, AM TI 4-DIMENSIONAL C-13/C-13-EDITED NUCLEAR OVERHAUSER ENHANCEMENT SPECTROSCOPY OF A PROTEIN IN SOLUTION - APPLICATION TO INTERLEUKIN 1-BETA SO BIOCHEMISTRY LA English DT Article ID 3-DIMENSIONAL NMR-SPECTROSCOPY; MAGNETIC-RESONANCE; HETERONUCLEAR NMR; DISTANCE GEOMETRY; SPECTRA; SIGNALS; DOMAIN AB A four-dimensional C-13/C-13-edited NOESY experiment is described which dramatically improves the resolution of protein NMR spectra and enables the straightforward assignment of nuclear Overhauser effects involving aliphatic and/or aromatic protons in larger proteins. The experiment is demonstrated for uniformly (> 95%) C-13-labeled interleukin 1-beta, a protein of 153 residues and 17.4 kDa, which plays a key role in the immune response. NOEs between aliphatic and/or aromatic protons are first spread out into a third dimension by the C-13 chemical shift of the carbon atom attached to the originating proton and subsequently into a fourth dimension by the C-13 chemical shift of the carbon atom attached to the destination proton. Thus, each NOE cross peak is labeled by four chemical shifts. By this means, ambiguities in the assignment of NOEs that arise from chemical shift overlap and degeneracy are completely removed. Further, NOEs between protons with the same chemical shifts can readily be detected providing their attached carbon atoms have different C-13 chemical shifts. The design of the pulse sequence requires special care to minimize the level of artifacts arising from undesired coherence transfer pathways, and in particular those associated with "diagonal" peaks which correspond to magnetization that has not been transferred from one proton to another. The 4D C-13/C-13-edited NOESY experiment is characterized by high sensitivity as the through-bond transfer steps involve the large J1CH (130 Hz) couplings, and it is possible to obtain high-quality spectra on 1-2 mM samples of C-13-labeled protein in as little as 3 days. This experiment should open up the application of protein structure determination by NMR to a large number of medium-sized proteins (150-300 residues) of biological interest. RP CLORE, GM (reprint author), NIDDKD,CHEM PHYS LAB,BLDG 2,BETHESDA,MD 20892, USA. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 40 TC 156 Z9 156 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD JAN 8 PY 1991 VL 30 IS 1 BP 12 EP 18 DI 10.1021/bi00215a002 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ984 UT WOS:A1991EQ98400002 PM 1988012 ER PT J AU LENFANT, C HIGGINS, M AF LENFANT, C HIGGINS, M TI DEMOGRAPHIC-TRENDS AND BURDEN OF CARDIOVASCULAR-DISEASES IN THE UNITED-STATES SO BULLETIN DE L ACADEMIE NATIONALE DE MEDECINE LA French DT Article DE CARDIOVASCULAR DISEASES; UNITED-STATES; RISK FACTORS; PREVENTION AND CONTROL; MORTALITY; THERAPY AB The current burden of cardiovascular disease in the U.S. population and recent trends in morbidity, mortality and risk factors provide a perspective on heart disease in the 21st century. Projections of demographic trends for populations and predictions of the frequency, distribution and characteristics of cardiovascular disease in the future are offered with numerous reservations and subject to revision. Nonetheless, we can expect to see more patients with cardiovascular disease in the next few decades and these patients are likely to be older and to be from the less well-educated and poorer socioeconomic segments of society. Improvements in treatment for the initial cardiovascular event may result in increased survival of women and men suffering permanent damage or disability. There will also be better opportunities to prevent cardiovascular diseases through modifying risk factors in the general population and in high risk individuals. Non-invasive procedures will also increase opportunities for detecting and reversing preclinical atherosclerosis through hygienic and therapeutic measures. RP LENFANT, C (reprint author), NHLBI,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIE NATL DE MEDECINE PI PARIS 06 PA 16 RUE BONAPARTE, 75272 PARIS 06, FRANCE SN 0001-4079 J9 B ACAD NAT MED PARIS JI Bull. Acad. Natl. Med. PD JAN 8 PY 1991 VL 175 IS 1 BP 103 EP 119 PG 17 WC Medicine, General & Internal SC General & Internal Medicine GA FN368 UT WOS:A1991FN36800015 PM 1863852 ER PT J AU HUFF, JE HASEMAN, JK AF HUFF, JE HASEMAN, JK TI EXPOSURE TO CERTAIN PESTICIDES MAY POSE REAL CARCINOGENIC RISK SO CHEMICAL & ENGINEERING NEWS LA English DT Article C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 6 TC 6 Z9 6 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0009-2347 J9 CHEM ENG NEWS JI Chem. Eng. News PD JAN 7 PY 1991 VL 69 IS 1 BP 33 EP 36 PG 4 WC Chemistry, Multidisciplinary; Engineering, Chemical SC Chemistry; Engineering GA EQ858 UT WOS:A1991EQ85800019 ER PT J AU SCHNEIDER, TD AF SCHNEIDER, TD TI THEORY OF MOLECULAR MACHINES .1. CHANNEL CAPACITY OF MOLECULAR MACHINES SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article ID PROTEIN-PROTEIN INTERACTIONS; NUCLEAR MAGNETIC-RESONANCE; GENE ACTIVATOR PROTEIN; ESCHERICHIA-COLI; RESTRICTION ENDONUCLEASE; TRANSFER-RNA; MYOSIN SUBFRAGMENT-1; NUCLEOTIDE-SEQUENCE; ECORI ENDONUCLEASE; BINDING-SITES RP SCHNEIDER, TD (reprint author), NCI, FREDERICK CANC RES & DEV CTR, MATH BIOL LAB, POB B, FREDERICK, MD 21702 USA. OI Schneider, Thomas/0000-0002-9841-1531 FU NIGMS NIH HHS [GM28755] NR 128 TC 62 Z9 62 U1 1 U2 3 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-5193 J9 J THEOR BIOL JI J. Theor. Biol. PD JAN 7 PY 1991 VL 148 IS 1 BP 83 EP 123 DI 10.1016/S0022-5193(05)80466-7 PG 41 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA ET662 UT WOS:A1991ET66200005 PM 2016886 ER PT J AU SCHNEIDER, TD AF SCHNEIDER, TD TI THEORY OF MOLECULAR MACHINES .2. ENERGY-DISSIPATION FROM MOLECULAR MACHINES SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article ID DEOXYNUCLEOSIDE PHOSPHORAMIDITES; DEOXYPOLYNUCLEOTIDE SYNTHESIS; COMPUTATION; INFORMATION; COMPUTER; DEVICE; LOGIC RP SCHNEIDER, TD (reprint author), NCI,FREDERICK CANC RES & DEV CTR,MATH BIOL LAB,POB B,FREDERICK,MD 21702, USA. OI Schneider, Thomas/0000-0002-9841-1531 FU NIGMS NIH HHS [GM28755] NR 76 TC 60 Z9 60 U1 2 U2 9 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-5193 J9 J THEOR BIOL JI J. Theor. Biol. PD JAN 7 PY 1991 VL 148 IS 1 BP 125 EP 137 DI 10.1016/S0022-5193(05)80467-9 PG 13 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA ET662 UT WOS:A1991ET66200006 PM 2016881 ER PT J AU BLUMENSTEIN, JJ MICHEJDA, CJ AF BLUMENSTEIN, JJ MICHEJDA, CJ TI BISTRIAZENES - MULTIFUNCTIONAL ALKYLATING-AGENTS SO TETRAHEDRON LETTERS LA English DT Article ID DECOMPOSITION; 1,3-DIALKYLTRIAZENES; TRIALKYLTRIAZENES; RATS AB The synthesis of bis(methyltriazeno)alkanes is readily accomplished by reaction of methyllithium with the appropriate diazidoalkane. These compounds possess interesting properties in solution, suggestive of an array of intermolecular hydrogen bonding. RP BLUMENSTEIN, JJ (reprint author), NCI,FREDERICK CANC RES & DEV CTR,CHEM & PHYS CARCINOGENESIS LAB,FREDERICK,MD 21701, USA. NR 17 TC 13 Z9 13 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0040-4039 J9 TETRAHEDRON LETT JI Tetrahedron Lett. PD JAN 7 PY 1991 VL 32 IS 2 BP 183 EP 186 DI 10.1016/0040-4039(91)80849-2 PG 4 WC Chemistry, Organic SC Chemistry GA ET347 UT WOS:A1991ET34700008 ER PT J AU SALTIS, J HABBERFIELD, AD EGAN, JJ LONDOS, C SIMPSON, IA CUSHMAN, SW AF SALTIS, J HABBERFIELD, AD EGAN, JJ LONDOS, C SIMPSON, IA CUSHMAN, SW TI ROLE OF PROTEIN-KINASE-C IN THE REGULATION OF GLUCOSE-TRANSPORT IN THE RAT ADIPOSE CELL - TRANSLOCATION OF GLUCOSE TRANSPORTERS WITHOUT STIMULATION OF GLUCOSE-TRANSPORT ACTIVITY SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PYRUVATE-DEHYDROGENASE ACTIVITY; INSULIN-RESPONSIVE TISSUES; BC3H-1 CULTURED MYOCYTES; PHORBOL ESTER; FAT-CELLS; PLASMA-MEMBRANE; PHOSPHOINOSITIDE METABOLISM; PHOSPHOLIPID-METABOLISM; POTENTIAL MECHANISM; INCREASES MEMBRANE AB The possible role of protein kinase C in the regulation of glucose transport in the rat adipose cell has been examined. Both insulin and phorbol 12-myristate 13-acetate (PMA) stimulate 3-O-methylglucose transport in the intact cell ein association with the subcellular redistribution of glucose transporters from the low density microsomes to the plasma membranes, as assessed by cytochalasin B binding. In addition, the actions of insulin of PMA on glucose transport activity and glucose transporter redistribution are additive. Furthermore, PMA accelerates insulin's stimulation of glucose transport activity, reducing the t1/2 from 3.2 +/- 0.4 to 2.1 +/- 0.2 min (means +/- S.E.). However, the effect of PMA on glucose transport activity is almost-equal-to 10% of that for insulin whereas its effect on glucose transporter redistribution is almost-equal-to 50% of the insulin response. Immunoblots of the GLUT1 and GLUT4 glucose transporter isoforms in subcellular membrane fractions also demonstrate that the translocations of GLUT1 in response to PMA and insulin are of similar magnitude whereas the translocation of GLUT4 in response to insulin is markedly greater than that in response to PMA. Thus, glucose transport activity in the intact cell with PMA and insulin correlates more closely with the appearance of GLUT4 in the plasma membrane than cytochalasin B-assayable glucose transporters. Although these data do not clarify the potential role of protein kinase C in the mechanism of insulin action, they do suggest that the mechanism through which insulin and PMA stimulate glucose transport are distinct but interactive. C1 NIDDKD,MOLEC CELLULAR & NUTR ENDOCRINOL BRANCH,EXPTL DIABET METAB & NUTR SECT,BLDG 10,RM 5N102,BETHESDA,MD 20892. NIDDKD,CELLULAR & DEV BIOL LAB,MEMBRANE REGULAT SECT,BETHESDA,MD 20892. NR 68 TC 50 Z9 50 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 5 PY 1991 VL 266 IS 1 BP 261 EP 267 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ339 UT WOS:A1991EQ33900039 PM 1985898 ER PT J AU HAFFAR, BM HOCART, SJ COY, DH MANTEY, S CHIANG, HCV JENSEN, RT AF HAFFAR, BM HOCART, SJ COY, DH MANTEY, S CHIANG, HCV JENSEN, RT TI REDUCED PEPTIDE-BOND PSEUDOPEPTIDE ANALOGS OF SECRETIN - A NEW CLASS OF SECRETIN RECEPTOR ANTAGONISTS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID VASOACTIVE INTESTINAL PEPTIDE; PANCREATIC ACINAR-CELLS; GUINEA-PIG PANCREAS; TYROSINE-HYDROXYLASE; ADENOSINE 3',5'-CYCLIC-MONOPHOSPHATE; STRUCTURAL REQUIREMENTS; BIOLOGICAL-ACTIVITY; ADENYLATE-CYCLASE; DISPERSED ACINI; AMYLASE RELEASE AB The ability to assess the importance of secretin in various physiological processes is limited by the lack of specific potent antagonists. Recently, reduced peptide bond (psi) analogues of bombesin or substance P in which the -CONH- Bond is replaced by -CH2NH- are reported to be receptor antagonists. To attempt to develop a new class of secretin receptor antagonists, we have adopted a similar strategy with secretin and sequentially altered the eight NH2-terminal peptide bonds, the biological active portion of secretin. In guinea pig pancreatic acini, secretin caused a 75-fold increase in cyclic AMP (cAMP). Secretin inhibited I-125-secretin binding with a half-maximal effect at 7 nM. Each of the psi-analogues inhibited I-125-secretin binding. [psi-4,5]Secretin was the most potent, causing the half-maximal inhibition at 4-mu-M, and was 2-fold more potent than the [psi-1,2]secretin; 7-fold more than [psi-3,4]secretin, [psi-5,6]secretin, and [psi-8,9]secretin; 9-fold more than [psi-7,8]secretin; 13-fold more potent[psi-6,7]secretin, and 17-fold more than [psi-2,3]secretin. Secretin caused a half-maximal increase in cAMP at 1 nM. At concentrations up to 10-mu-M, ]psi-2,3[secretin, [psi-4,5]secretin, and [psi-8,0]secretin did not alter cAMP whereas [psi-1,2]secretin and [psi-6,7]secretin caused a detectable increase in cAMP at 10 nM, [psi-7,8]secretin at 300 nM, [psi-5,6]secretin at 1-mu-M, and [psi-3,4]secretin at 10-mu-M. The [psi-4,5], [psi-2,3], and [psi8,9] analogues of secretin each inhibited 1 nM secretin-stimulated cAMP as well as [psi-3,4]secretin, which functioned as a partial agonist. [psi-4,5]Secretin was the most potent, causing half-maximal inhibition at 3-mu-M whereas [psi-8,9]secretin was 6-fold less potent, and [psi-2,3]secretin and [psi-3,4]secretin were 17-fold less potent. [psi-4,5]Secretin inhibited secretin-stimulated cAMP and binding of I-125-secretin in a competitive manner. [psi-4,5]Secretin did not interact with cholecystokinin, bombesin, calcitonin gene-related peptide, or cholinergic receptors but did interact with receptors for vasoactive intestinal peptide, causing half-maximal inhibition at 72-mu-M and thus had a 18-fold higher affinity for secretin than vasoactive intestinal peptide receptors. These results indicate that reduced peptide bond analogues of the NH2 terminus of secretin represent a new class of secretin receptor antagonists. It is likely that in the future even more potent members of this class can be developed which may be useful to investigate the role of secretin in various physiological processes. C1 TULANE UNIV,MED CTR,DEPT MED,PEPTIDE RES LABS,NEW ORLEANS,LA 70112. NIDDKD,DIGEST DIS BRANCH,BETHESDA,MD 20892. FU NIDDK NIH HHS [DK-30167] NR 65 TC 37 Z9 37 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 5 PY 1991 VL 266 IS 1 BP 316 EP 322 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ339 UT WOS:A1991EQ33900047 PM 1702423 ER PT J AU ACCILI, D MOSTHAF, L ULLRICH, A TAYLOR, SI AF ACCILI, D MOSTHAF, L ULLRICH, A TAYLOR, SI TI A MUTATION IN THE EXTRACELLULAR DOMAIN OF THE INSULIN-RECEPTOR IMPAIRS THE ABILITY OF INSULIN TO STIMULATE RECEPTOR AUTOPHOSPHORYLATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID FMS PROTO-ONCOGENE; GROWTH-FACTOR; 3T3-L1 ADIPOCYTES; TYROSINE KINASE; PLASMA-MEMBRANE; INTACT-CELLS; PHOSPHORYLATION; SUBUNITS; IDENTIFICATION; GLYCOSYLATION AB Mutations of the insulin receptor gene have been shown to cause insulin-resistant diabetes in patients with genetic forms of insulin resistance. We have previously reported that a mutation substituting valine for Phe382 in the alpha-subunit of the insulin receptor is associated with impaired transport of the mutant receptor to the plasma membrane (Accili, D., Frapier, C., Mosthaf, L., McKeon, C., Elbein, S. C., Permutt, M. A., Ramos, E., Lander, E. S., Ullrich, A., and Taylor, S. I. (1989) EMBO J. 8, 2509-2517). In this study, we demonstrate that the Val382 mutation impairs the ability of insulin to activate receptor autophosphorylation. Furthermore, the Val382 receptor has reduced activity to phosphorylate other peptide substrates in the presence of insulin. Nevertheless, when the Val382 mutant and wild-type receptor, thereby activating the tyrosine kinase activity of the mutant receptor. Thus, the conformational change used by the Val382 mutation compromises the ability of the receptor to transmit a signal across the plasma membrane. Furthermore, our observations suggest that receptor phosphorylation by an intermolecular mechanism (i.e. transphosphorylation) may play a role in mediating the action of insulin upon the target cell. C1 MAX PLANCK INST BIOCHEM,DEPT MOLEC BIOL,W-8033 MARTINSRIED,GERMANY. RP ACCILI, D (reprint author), NIDDKD,DIABET BRANCH,BIOCHEM & MOLEC PATHOPHYSIOL SECT,BETHESDA,MD 20892, USA. NR 35 TC 42 Z9 42 U1 1 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 5 PY 1991 VL 266 IS 1 BP 434 EP 439 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ339 UT WOS:A1991EQ33900063 PM 1845971 ER PT J AU TAHARA, K GROLLMAN, EF SAJI, M KOHN, LD AF TAHARA, K GROLLMAN, EF SAJI, M KOHN, LD TI REGULATION OF PROSTAGLANDIN SYNTHESIS BY THYROTROPIN, INSULIN OR INSULIN-LIKE GROWTH FACTOR-I, AND SERUM IN FRTL-5 RAT-THYROID CELLS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ARACHIDONIC-ACID METABOLISM; MESSENGER-RNA LEVELS; ENDOPEROXIDE SYNTHASE; ALPHA-1-ADRENERGIC STIMULATION; FORMING CYCLOOXYGENASE; COMPLEMENTARY-DNA; GENE-EXPRESSION; 3T3 FIBROBLASTS; G/H SYNTHASE; PURIFICATION AB The present report shows that thyrotropin (TSH) regulates all three steps involved in prostaglandin synthesis in FRTL-5 rat thyroid cells, i.e. arachidonic acid release from membrane phospholipids, cyclooxygenase (prostaglandin H synthase) action, and individual prostaglandin formation; however, its action at specific steps may require the presence of, or can be duplicated by, insulin, insulin-like growth factor-I (IGF-I), and/or a serum factor. Thus, TSH releases free arachidonic acid from rat FRTL-5 thyroid cells whose phospholipid fraction is radiolabeled with [H-3]arachidonic acid; this action involves a pertussis toxin-sensitive G protein, is not cAMP mediated, and does not require insulin or 5% serum. To quantitate TSH effects on cyclooxygenase activity and on individual prostaglandin formation, a homogenate system and a rapid reversed-phase high pressure liquid chromatography procedure have been developed to measure cyclooxygenase metabolites. TSH increased cyclooxygenase activity in homogenates only if the cells were also exposed to insulin, IGF-I, and/or 5% calf serum; TSH alone had no apparent effect on the activity. Maximal activation, 4-fold over basal/mu-g of DNA, took 36 h to achieve and reflected, at least in part, an increase in cyclooxygenase gene expression. Like cyclooxygenase activity, induction of prostaglandin E2 production required 2 or more factors, i.e. TSH plus insulin/IGF-I or TSH plus insulin/IGF-I plus serum. Increased production of prostaglandin D2, could, however, be detected if cells were treated with TSH alone and the TSH activity could be duplicated by insulin, IGF-I, or calf serum alone. RP TAHARA, K (reprint author), NIDDKD,BIOCHEM & METAB LAB,CELL REGULAT SECT,BETHESDA,MD 20892, USA. RI Saji, Motoyasu/E-4007-2011 NR 67 TC 50 Z9 50 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 5 PY 1991 VL 266 IS 1 BP 440 EP 448 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ339 UT WOS:A1991EQ33900064 PM 1845972 ER PT J AU DICHEK, HL FOJO, SS BEG, OU SKARLATOS, SI BRUNZELL, JD CUTLER, GB BREWER, HB AF DICHEK, HL FOJO, SS BEG, OU SKARLATOS, SI BRUNZELL, JD CUTLER, GB BREWER, HB TI IDENTIFICATION OF 2 SEPARATE ALLELIC MUTATIONS IN THE LIPOPROTEIN-LIPASE GENE OF A PATIENT WITH THE FAMILIAL HYPERCHYLOMICRONEMIA SYNDROME SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MOLECULAR-CLONING; SEQUENCE; DNA; DEFICIENCY; EVOLUTION; PROTEIN; ENZYME AB The molecular defects resulting in a deficiency of lipoprotein lipase activity in a patient with the familial hyperchylomicronemia syndrome have been identified. Increased lipoprotein lipase mass but undetectable lipoprotein lipase activity in the patient's post-heparin plasma indicate the presence of an inactive enzyme. No major gene rearrangements were identified by Southern blot analysis of the patient's lipoprotein lipase gene and Northern blot hybridization revealed an lipoprotein lipase mRNA of normal size. Sequence analysis of polymerase chain reaction-amplified lipoprotein lipase cDNA identified two separate allelic mutations. A T to C transition at nucleotide 836 results in the substitution of Ile194, located near the putative interfacial recognition site of lipoprotein lipase, to a Thr. A G to A mutation at base 983 leads to the substitution of a His a for Arg243 and the loss of a HhaI restriction enzyme site. Arg243 is near His241, which has been postulated to be part of the catalytic triad of lipoprotein lipase. Direct sequencing of amplified cDNA and digestion with HhaI established that the proband is a compound heterozygote for each base substitution. Transient expression of each of the mutant lipoprotein lipase cDNAs in human embryonal kidney-293 cells resulted in the synthesis of enzymically inactive proteins, establishing the functional significance of the mutations. We conclude that the Ile194 to Thr194 and Arg243 to His243 substitutions occur in lipoprotein lipase regions essential for normal enzyme activity and each mutation results in the expression of a nonfunctional enzyme leading to the hyperchylomicronemia syndrome manifested in the proband. C1 NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. UNIV WASHINGTON,DEPT MED,DIV METAB ENDOCRINOL & NUTR,SEATTLE,WA 98195. RP DICHEK, HL (reprint author), NHLBI,MOLEC DIS BRANCH,BETHESDA,MD 20892, USA. FU NIDDK NIH HHS [DK-02456] NR 30 TC 53 Z9 54 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 5 PY 1991 VL 266 IS 1 BP 473 EP 477 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ339 UT WOS:A1991EQ33900069 PM 1702428 ER PT J AU KIM, HC IDLER, WW KIM, IG HAN, JH CHUNG, SI STEINERT, PM AF KIM, HC IDLER, WW KIM, IG HAN, JH CHUNG, SI STEINERT, PM TI THE COMPLETE AMINO-ACID-SEQUENCE OF THE HUMAN TRANSGLUTAMINASE-K ENZYME DEDUCED FROM THE NUCLEIC-ACID SEQUENCES OF CDNA CLONES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID CROSS-LINKED ENVELOPE; MOUSE EPIDERMAL-CELLS; FACTOR-XIII; CORNIFIED ENVELOPE; HAIR FOLLICLE; GUINEA-PIG; KERATINOCYTE TRANSGLUTAMINASE; PROTEIN; DIFFERENTIATION; PURIFICATION AB In order to study the expression and role of transglutaminases in the formation of the cross-linked cell envelope of human epidermis, we have used a synthetic oligonucleotide encoding the consensual active site sequence of known transglutaminase sequences. By Northern blot analysis, newborn foreskin epidermis expresses three different mRNa species of about 3.7, 3.3, and 2.9 kilobases while normal cultured epidermal keratinocytes express only the 3.7- and 2.9-kilobase species. The largest species corresponds to a known ubiquitous tissue type II or transglutaminase C activity, the smallest corresponds to a known type I or transglutaminase K activity, and the mid-sized component apparently encodes a transglutaminase E activity that has recently been shown to be expressed in terminally differentiating epidermis (Kim, H. C., Lewis, M.S., Gorman, J.L., Park, S.C., Girard, J.E., Folk, J.E. & Chung, S.I. (1990) J. Biol. Chem., in press). Using the active site oligonucleotide as a probe, we have isolated and sequenced cDNA clones encoding the transglutaminase K enzyme. The deduced complete protein sequence has 813-amino acid residues of 89.3 kDa, has a pl of 5.7, and is likely to be an essentially globular protein, which are properties expected from the partially purified enzyme. It shares 49-53% sequence homology with the other transglutaminases of known sequence, especially in regions carboxyl-terminal to the active site, and possesses sequences likely to confer its Ca2+ dependence. Interestingly, its larger size is due to extended sequences on its amino and carboxyl termini, absent on the other transglutaminases, that may define its unique properties. C1 NIAMSD,SKIN BIOL LAB,BLDG 10,RM 12N238,BETHESDA,MD 20892. NIDA,CELLULAR DEV & ONCOL LAB,BETHESDA,MD 20892. NR 43 TC 94 Z9 96 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 5 PY 1991 VL 266 IS 1 BP 536 EP 539 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ339 UT WOS:A1991EQ33900079 PM 1670769 ER PT J AU CRIPPA, MP NICKOL, JM BUSTIN, M AF CRIPPA, MP NICKOL, JM BUSTIN, M TI DIFFERENTIATION-DEPENDENT ALTERATION IN THE CHROMATIN STRUCTURE OF CHROMOSOMAL PROTEIN HMG-17 GENE DURING ERYTHROPOIESIS SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article ID MOBILITY GROUP PROTEIN-14; HYPERSENSITIVE SITES; TRANSCRIBED GENES; ACTIVE CHROMATIN; MULTIGENE FAMILY; FUNCTIONAL GENE; 1ST INTRON; LOCALIZATION; SEQUENCES; IDENTIFICATION C1 NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. NIDDK,MOLEC BIOL LAB,BETHESDA,MD 20892. RI crippa, massimo/J-6514-2016; Bustin, Michael/G-6155-2015 OI crippa, massimo/0000-0003-3214-9670; NR 38 TC 8 Z9 8 U1 0 U2 1 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD JAN 5 PY 1991 VL 217 IS 1 BP 75 EP 84 DI 10.1016/0022-2836(91)90612-A PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EU018 UT WOS:A1991EU01800010 PM 1988681 ER PT J AU KLINE, RL BROTHERS, T HALSEY, N BOULOS, R LAIRMORE, MD QUINN, TC AF KLINE, RL BROTHERS, T HALSEY, N BOULOS, R LAIRMORE, MD QUINN, TC TI EVALUATION OF ENZYME IMMUNOASSAYS FOR ANTIBODY TO HUMAN T-LYMPHOTROPIC VIRUSES TYPE-I/II SO LANCET LA English DT Article ID CELL LEUKEMIA-VIRUS; CHRONIC PROGRESSIVE MYELOPATHY; HTLV-I; LYMPHOMA VIRUS; BLOOD; TRANSMISSION; INFECTION; ANTIGEN AB To evaluate the sensitivity and specificity of HTLV-I/II assays, serum from 1100 pregnant Haitian women was tested with seven commercially available HTLV I/II assays. Serum that was found to be reactive in any assay was analysed by western blot and all indeterminate samples were further characterised by radioimmunoprecipitation assays (RIPA). 59 (5.4%) samples were HTLVI/II antibody positive by western blot and/or RIPA. The sensitivity of these seven assays ranged from 93.2% to 100%, with the 'Recombinant HTLV-I' (Cambridge Bioscience) and 'Serodia' HTLV-I' (Fujirebio) assays having the highest sensitivity (100%). The specificity of these assays ranged from 98.4% to 100%, with the Abbott assay having the highest specificity (99.5%), 100%) according to two different methods of evaluation. Whether the antigens used in any assay were whole disrupted virus or recombinant gene products made no difference. The low positive predictive values of some of these assays (71.8-91.7%), even in a high prevalence population, and the need for RIPA to test indeterminate sera, indicate that for routine screening of blood donors there is still room for improvement both in screening and confirmatory assays for HTLV-I/II. C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,SCH MED,DIV INFECT DIS,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT INT HLTH,BALTIMORE,MD 21218. CTR DEV & HLTH,PORT AU PRINCE,HAITI. CTR DIS CONTROL,RETROVIRUS DIS BRANCH,ATLANTA,GA 30333. FU PHS HHS [R0IA126521] NR 25 TC 25 Z9 26 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD JAN 5 PY 1991 VL 337 IS 8732 BP 30 EP 33 DI 10.1016/0140-6736(91)93343-8 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA EQ604 UT WOS:A1991EQ60400017 PM 1670658 ER PT J AU MARKWELL, MAK SHENG, HZ BRENNEMAN, DE PAUL, SM AF MARKWELL, MAK SHENG, HZ BRENNEMAN, DE PAUL, SM TI A RAPID METHOD TO QUANTIFY NEURONS IN MIXED CULTURES BASED ON THE SPECIFIC BINDING OF [H-3] OUABAIN TO NEURONAL NA+,K+-ATPASE SO BRAIN RESEARCH LA English DT Article DE NA+,K+-ATPASE; OUABAIN; NEUROTOXICITY; HIPPOCAMPUS; KAINATE; N-METHYL-D-ASPARTATE; PHENCYCLIDINE ID CENTRAL NERVOUS-SYSTEM; OUABAIN BINDING; HIGH-AFFINITY; HUMAN-BRAIN; ATPASE; CELLS AB The high-affinity binding of [H-3]ouabain to Na+, K+-ATPase was characterized in primary cultures of hippocampal neurons grown on feeder layers of astrocytes. The specific binding of [H-3]ouabain was found to be time-dependent, high-affinity (apparent K(d) = 8.5 nM), saturable B(max) = 20.6 pmol/mg protein), dependent upon the presence of ATP, inhibited by K+, and directly proportional to neuronal, but not glial, cell number. Similar results were obtained using either sonicated cell suspensions or intact whole cells in culture. At the concentration of neurons routinely used, the specific binding of [H-3]ouabain to the astrocyte feeder layer constituted less than 10% of total specific binding. Agents that selectively kill neurons rather than glia, such as the excitotoxins N-methyl-D-aspartate (NMDA) and kainate, reduced the amount of [H-3]ouabain specifically bound in mixed cultures in a time- and concentration-dependent manner. Measurement of high-affinity [H-3]ouabain binding to the neuronal form of Na+, K+-ATPase provides a simple, rapid, and reproducible method to quantify neurons in mixed culture. C1 NICHHD,DEV NEUROBIOL LAB,BETHESDA,MD 20892. RP MARKWELL, MAK (reprint author), NIMH,CLIN NEUROSCI BRANCH,MOLEC PHARMACOL SECT,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 27 TC 22 Z9 22 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD JAN 4 PY 1991 VL 538 IS 1 BP 1 EP 8 DI 10.1016/0006-8993(91)90368-6 PG 8 WC Neurosciences SC Neurosciences & Neurology GA EP963 UT WOS:A1991EP96300001 PM 1850316 ER PT J AU NEWMAN, JD WINSLOW, JT MURPHY, DL AF NEWMAN, JD WINSLOW, JT MURPHY, DL TI MODULATION OF VOCAL AND NONVOCAL BEHAVIOR IN ADULT SQUIRREL-MONKEYS BY SELECTIVE MAO-A AND MAO-B INHIBITION SO BRAIN RESEARCH LA English DT Article DE MONOAMINE OXIDASE INHIBITION; PRIMATES; VOCALIZATION; ETHOPHARMACOLOGY; MILACEMIDE; DEPRENYL; CLORGYLINE ID MONOAMINE-OXIDASE ACTIVITY; CEREBROSPINAL-FLUID; RAT-BRAIN; CLORGYLINE; CLONIDINE; NOREPINEPHRINE; CATECHOLAMINE; METABOLITES; MILACEMIDE; DISTRESS AB The acute effects of monoamine oxidase inhibitors L-deprenyl (0.5-5.0 mg/kg), clorgyline (1.0-10.0 mg/kg), and milacemide (100-400 mg/kg) on the behavior of adult male squirrel monkeys were examined during brief social separations beginning 60 min after subcutaneous drug administration. All three drugs selectively reduced the rate of calling during social separation at doses which did not affect time spent in locomotion, nor the frequency of vigilance-checking. Deprenyl and milacemide, but not clorgyline, produced concurrent decreases in locomotion at the higher doses tested. At threshold doses, clorgyline, but not deprenyl or milacemide, increased call duration and decreased call peak frequency compared to vehicle control values. Plasma levels of MHPG were decreased by an optimal dose of clorgyline but not by deprenyl or milacemide, indicating that substrate specificity was maintained at the drug doses employed. We conclude that different MAO substrates mediate different aspects of vocal and nonvocal behavior in adult male squirrel monkeys. C1 NICHHD,COMPARAT ETHOL LAB,BETHESDA,MD 20892. NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NR 32 TC 8 Z9 8 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD JAN 4 PY 1991 VL 538 IS 1 BP 24 EP 28 DI 10.1016/0006-8993(91)90371-2 PG 5 WC Neurosciences SC Neurosciences & Neurology GA EP963 UT WOS:A1991EP96300004 PM 1902133 ER PT J AU EHRENREICH, H KEHRL, JH ANDERSON, RW RIECKMANN, P VITKOVIC, L COLIGAN, JE FAUCI, AS AF EHRENREICH, H KEHRL, JH ANDERSON, RW RIECKMANN, P VITKOVIC, L COLIGAN, JE FAUCI, AS TI A VASOACTIVE PEPTIDE, ENDOTHELIN-3, IS PRODUCED BY AND SPECIFICALLY BINDS TO PRIMARY ASTROCYTES SO BRAIN RESEARCH LA English DT Article DE ASTROCYTE; GLIAL CELL; BRAIN; ENDOTHELIN-3; VASOACTIVE PEPTIDE; ENDOTHELIN-RECEPTOR ID VASOCONSTRICTOR PEPTIDE; GLUTAMINE-SYNTHETASE; BIOLOGICAL-ACTIVITY; CEREBRAL VASOSPASM; SPINAL-CORD; BRAIN; CELLS; RAT; VISUALIZATION; LOCALIZATION AB Primary rat astrocytes were found by immunohistochemistry to display positive staining for endothelin-3, located predominantly in the perinuclear area. The ability of these cells to produce and release endothelin-3 was confirmed by a combination of reverse-phase HPLC and radioimmunoassay, specific for endothelin-3, which demonstrated immunoreactive peptide in cellular extracts and astrocyte-conditioned medium. In addition, astrocytes were shown to possess a single class of binding sites for endothelin with comparable high affinity for endothelin-1, -2 and -3. These results suggest that astrocytes, by virtue of their ability to produce and secrete endothelin-3, serve as a potential extravascular source of intracerebral vasoregulation capable of influencing regional cerebral blood flow. C1 NIAID,BIOL RESOURCES BRANCH,BETHESDA,MD 20892. RP EHRENREICH, H (reprint author), NIAID,IMMUNOREGULAT LAB,BLDG 10,RM 11B-13,BETHESDA,MD 20892, USA. NR 37 TC 122 Z9 123 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD JAN 4 PY 1991 VL 538 IS 1 BP 54 EP 58 DI 10.1016/0006-8993(91)90375-6 PG 5 WC Neurosciences SC Neurosciences & Neurology GA EP963 UT WOS:A1991EP96300008 PM 1850319 ER PT J AU MOFFETT, JR WILLIAMSON, LC NEALE, JH PALKOVITS, M NAMBOODIRI, MAA AF MOFFETT, JR WILLIAMSON, LC NEALE, JH PALKOVITS, M NAMBOODIRI, MAA TI EFFECT OF OPTIC-NERVE TRANSECTION ON N-ACETYLASPARTYLGLUTAMATE IMMUNOREACTIVITY IN THE PRIMARY AND ACCESSORY OPTIC PROJECTION SYSTEMS IN THE RAT SO BRAIN RESEARCH LA English DT Article DE LATERAL GENICULATE NUCLEUS; SUPERIOR COLLICULUS; NUCLEUS OF THE OPTIC TRACT; MEDIAL TERMINAL NUCLEUS; NEUROPEPTIDE; RADIOIMMUNOASSAY ID LATERAL GENICULATE-NUCLEUS; ASPARTYL-L-GLUTAMATE; ACIDIC DIPEPTIDASE ACTIVITY; KAINIC ACID; SUPRACHIASMATIC NUCLEUS; VISUAL-SYSTEM; ACETYL-ASPARTYLGLUTAMATE; RECEPTOR ANTAGONISTS; GANGLION-CELLS; EVOKED RELEASE AB Evidence has been presented in recent years that support the hypothesis that N-acetylaspartylglutamate (NAAG) may be involved in synaptic transmission in the optic tract of mammals. Using a modified fixation protocol, we have determined the detailed distribution of NAAG immunoreactivity (NAAG-IR) in retinal ganglion cells and optic projections of the rat. Following optic nerve transection, dramatic losses of NAAG-IR were observed in the neuropil of all retinal target zones including the lateral geniculate nucleus, superior colliculus, nucleus of the optic tract, the dorsal and medial terminal nuclei and suprachiasmatic nucleus. Brain regions were microdissected and NAAG levels measured by a radioimmunoassay (RIA) (IC50:NAAG = 2.5 nM, NAA = 100-mu-M; smallest detectable amount = 1-2 pg/assay). Large decreases (50-60%) in NAAG levels were detected in the lateral geniculate, superior colliculus and suprachiasmatic nucleus. Moderate losses (25-45%) were noted in the pretectal nucleus and the nucleus of the optic tract. Smaller changes (15-20%) were detected in the paraventricular nucleus and the pretectal area. These results are consistent with a synaptic communication role for NAAG in the visual system. C1 NIMH,CELL BIOL LAB,BETHESDA,MD 20892. RP MOFFETT, JR (reprint author), GEORGETOWN UNIV,DEPT BIOL,37TH & O STS NW,WASHINGTON,DC 20057, USA. RI Palkovits, Miklos/F-2707-2013 FU NIDA NIH HHS [DA02297]; NIDDK NIH HHS [DK37024]; NINDS NIH HHS [NS 28130] NR 46 TC 41 Z9 41 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD JAN 4 PY 1991 VL 538 IS 1 BP 86 EP 94 DI 10.1016/0006-8993(91)90380-E PG 9 WC Neurosciences SC Neurosciences & Neurology GA EP963 UT WOS:A1991EP96300013 PM 2018935 ER PT J AU SAYER, JM CHADHA, A AGARWAL, SK YEH, HJC YAGI, H JERINA, DM AF SAYER, JM CHADHA, A AGARWAL, SK YEH, HJC YAGI, H JERINA, DM TI COVALENT NUCLEOSIDE ADDUCTS OF BENZO[A]PYRENE 7,8-DIOL 9,10-EPOXIDES - STRUCTURAL REINVESTIGATION AND CHARACTERIZATION OF A NOVEL ADENOSINE ADDUCT ON THE RIBOSE MOIETY SO JOURNAL OF ORGANIC CHEMISTRY LA English DT Article ID DIOL EPOXIDES; DNA ADDUCTS; ABSOLUTE STEREOCHEMISTRY; DEOXYADENOSINE ADDUCTS; 7,12-DIMETHYLBENZANTHRACENE; ACID; HYDROLYSIS; ADENINE; GUANINE; BENZOPHENANTHRENE AB The diastereomeric 7,8-diol 9,10-epoxides metabolically derived from the carcinogenic hydrocarbon benzo[alpha]pyrene react with the purine bases in nucleic acids to alkylate their exocyclic amino groups. The major adducts formed from polyguanylic acid and the enantiomers of diol epoxide-1 (the diastereomer in which the benzylic 7-hydroxyl group and the epoxide oxygen are cis) have been shown to result from cis opening of the epoxide by the N-2 amino group of guanine, rather than trans opening as had been previously reported. Four adducts resulting from alkylation of the exocyclic N-6 amino group of adenosine 5'-monophosphate by racemic diol epoxide-1 have been prepared and characterized. In addition, a major adduct formed from adenosine 5'-monophosphate and (-)-(7R,8S)-diol (9R,10S)-epoxide-1, but not from its (+) enantiomer, has been identified as a product of alkylation of the 2'-hydroxyl group of the sugar. We also report a quantitative reevaluation of the extent and distribution of covalent adduct formation from calf thymus DNA and both diastereomeric benzo[alpha]pyrene diol epoxides, as well as the identification of the principal DNA adducts formed from the enantiomers of diol epoxide-1. Tentative identification of several new minor adducts formed upon reaction of diol epoxide-2 with denatured DNA is described. The present results provide additional support for our previously proposed correlation between the signs of the circular dichroism bands of these adducts and their absolute configurations at the N-substituted benzylic carbon atom. C1 NIDDKD,BIOORGAN & ANALYT CHEM LAB,BETHESDA,MD 20892. NR 49 TC 142 Z9 143 U1 0 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-3263 J9 J ORG CHEM JI J. Org. Chem. PD JAN 4 PY 1991 VL 56 IS 1 BP 20 EP 29 DI 10.1021/jo00001a007 PG 10 WC Chemistry, Organic SC Chemistry GA EQ979 UT WOS:A1991EQ97900007 ER PT J AU RALL, DP AF RALL, DP TI CARCINOGENS AND HUMAN HEALTH .2. SO SCIENCE LA English DT Letter ID 327 CHEMICAL CARCINOGENICITY; NATIONAL TOXICOLOGY PROGRAM; MULTISTEP CARCINOGENESIS; OCCUPATIONAL-CANCER; MOLECULAR-BASES; 104 CHEMICALS; ANTIONCOGENES; ONCOGENES; TOXICITY C1 NATL TOXICOL PROGRAM,WASHINGTON,DC. RP RALL, DP (reprint author), NIEHS,5302 RENO RD,WASHINGTON,DC 20013, USA. NR 58 TC 18 Z9 18 U1 1 U2 2 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD JAN 4 PY 1991 VL 251 IS 4989 BP 10 EP 12 DI 10.1126/science.1986406 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EQ603 UT WOS:A1991EQ60300002 PM 1986406 ER PT J AU MIKI, T FLEMING, TP BOTTARO, DP RUBIN, JS RON, D AARONSON, SA AF MIKI, T FLEMING, TP BOTTARO, DP RUBIN, JS RON, D AARONSON, SA TI EXPRESSION CDNA CLONING OF THE KGF RECEPTOR BY CREATION OF A TRANSFORMING AUTOCRINE LOOP SO SCIENCE LA English DT Article ID GROWTH-FACTOR; CELLS; DIFFERENTIATION; PURIFICATION; LIBRARIES; INSERTS; SYSTEM AB An expression cloning strategy was devised to isolate the keratinocyte growth factor (KGF) receptor complementary DNA. NIH/3T3 fibroblasts, which secrete this epithelial cell-specific mitogen, were transfected with a keratinocyte expression complementary DNA library. Among several transformed foci identified, one demonstrated the acquisition of specific high-affinity KGF binding sites. The pattern of binding competition by related fibroblast growth factors (FGFs) indicated that this receptor had high affinity for acidic FGF as well as KGF. The rescued 4.2-kilobase complementary DNA was shown to encode a predicted membrane-spanning tyrosine kinase related to but distinct from the basic FGF receptor. This expression cloning approach may be generally applicable to the isolation of genes that constitute limiting steps in mitogenic signaling pathways. RP MIKI, T (reprint author), NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892, USA. RI Bottaro, Donald/F-8550-2010 OI Bottaro, Donald/0000-0002-5057-5334 NR 35 TC 433 Z9 441 U1 0 U2 3 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD JAN 4 PY 1991 VL 251 IS 4989 BP 72 EP 75 DI 10.1126/science.1846048 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EQ603 UT WOS:A1991EQ60300029 PM 1846048 ER PT J AU KOZLOWSKI, S TAKESHITA, T BOEHNCKE, WH TAKAHASHI, H BOYD, LF GERMAIN, RN BERZOFSKY, JA MARGULIES, DH AF KOZLOWSKI, S TAKESHITA, T BOEHNCKE, WH TAKAHASHI, H BOYD, LF GERMAIN, RN BERZOFSKY, JA MARGULIES, DH TI EXCESS BETA-2 MICROGLOBULIN PROMOTING FUNCTIONAL PEPTIDE ASSOCIATION WITH PURIFIED SOLUBLE CLASS-I MHC MOLECULES SO NATURE LA English DT Article ID MAJOR HISTOCOMPATIBILITY COMPLEX; TOXIC LYMPHOCYTES-T; ENVELOPE PROTEIN; CELL HYBRIDOMAS; ANTIGEN; HLA; BETA-2-MICROGLOBULIN; RECOGNITION; H-2; DISSOCIATION AB T LYMPHOCYTES expressing alpha-beta-receptors recognize antigenic peptide fragments bound to major histocompatibility complex class I (ref. 1) or class II (ref. 2) molecules present on the surface membranes of other cells. Peptide fragments are present in the two available HLA crystal structures3,4 and recent data indicate that peptide is required for the stable folding of the class I heavy chain and maintenance of its association with the class I light chain beta-2-microglobulin (beta-2-m), at physiological temperature5-7. To explain how the exogeneous peptide used to create targets for cytotoxic cells bearing CD8 antigen1 could associate with apparently peptide-filled extracellular class I molecules, we hypothesized that stable binding of exogenous peptide to mature class I molecules reflects either the replacement of previously bound peptide during the well documented beta-2m exchange process8 or the loading of 'empty' class I heavy chains dependent on the availability of excess beta-2m. In either case, free beta-2m should enhance peptide-class I binding. Using either isolated soluble class I molecules or living cells, we show here that free purified beta-2m markedly augments the generation of antigenic complexes capable of T-cell stimulation. C1 NIAID,IMMUNOL LAB,LYMPHOCYTE BIOL SECT,BETHESDA,MD 20892. NCI,METAB BRANCH,MOLEC IMMUNOGENET & VACCINE RES SECT,BETHESDA,MD 20892. RP KOZLOWSKI, S (reprint author), NIAID,IMMUNOL LAB,MOLEC BIOL SECT,BETHESDA,MD 20892, USA. RI Margulies, David/H-7089-2013; OI Margulies, David/0000-0001-8530-7375 NR 23 TC 144 Z9 152 U1 0 U2 0 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD JAN 3 PY 1991 VL 349 IS 6304 BP 74 EP 77 DI 10.1038/349074a0 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EQ601 UT WOS:A1991EQ60100055 PM 1985269 ER PT J AU VINEIS, P CAPORASO, N AF VINEIS, P CAPORASO, N TI THE ANALYSIS OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM IN HUMAN CANCER - A REVIEW FROM AN EPIDEMIOLOGIC PERSPECTIVE SO INTERNATIONAL JOURNAL OF CANCER LA English DT Review ID HUMAN-LUNG CANCER; RARE HA-RAS; L-MYC GENE; PROTO-ONCOGENE; HYPERVARIABLE ALLELES; MYELODYSPLASIA; METASTASIS; FREQUENCY; MELANOMA; DISEASE AB We have reviewed published case-control studies on the role of Restriction Fragment Length Polymorphism (RFLP) for specific loci in the causation of human cancer or as a prognostic factor. Five studies have been published on L-myc polymorphism and prognosis for several types of cancer (lung, kidney, stomach or breast). Sixteen studies report on Ha-ras polymorphism and the risk of cancers in the lung, breast, bladder, colorectum, brain, leukemias and melanoma. The results of the studies are conflicting. In addition, such studies raise important methodological issues: the choice of the cut-off between "rare" and "common" alleles; the inclusion of prevalent cases; the distribution of the alleles in subgroups of the population, including different ethnic groups; a mechanistic interpretation of the role played by the polymorphism, including possible interaction with environmental exposures. These questions need to be answered before a cause-effect relationship can be clearly established. C1 UNIV TURIN,I-10126 TURIN,ITALY. NCI,FAMILY STUDIES SECT,BETHESDA,MD 20892. RP VINEIS, P (reprint author), MAIN HOSP,CANC EPIDEMIOL UNIT,VIA SANTENA 7,I-10126 TURIN,ITALY. NR 29 TC 23 Z9 23 U1 0 U2 3 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD JAN 2 PY 1991 VL 47 IS 1 BP 26 EP 30 DI 10.1002/ijc.2910470106 PG 5 WC Oncology SC Oncology GA ET492 UT WOS:A1991ET49200005 PM 1670765 ER PT J AU CASABONA, J MELBYE, M BIGGAR, RJ AF CASABONA, J MELBYE, M BIGGAR, RJ TI KAPOSIS-SARCOMA AND NON-HODGKINS-LYMPHOMA IN EUROPEAN AIDS CASES - NO EXCESS RISK OF KAPOSIS-SARCOMA IN MEDITERRANEAN COUNTRIES SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID ACQUIRED IMMUNODEFICIENCY SYNDROME; HOMOSEXUAL MEN; SEXUAL-BEHAVIOR; HIV INFECTION; ASSOCIATION; COFACTORS; CANCER; STATES; TRENDS; UGANDA AB Prior to the AIDS epidemic, Kaposi's sarcoma (non-AIDS-KS) in Europe was mainly a disease of elderly Mediterranean men. In 1989 AIDS data from 15 European countries were collected to study proportional trends in AIDS-related Kaposi's sarcoma (AIDS-KS) in order to determine whether specific factors in Southern Europe might be important in the development of KS among AIDS patients. Another AIDS-related cancer, non-Hodgkin's lymphoma (NHL) was included as a malignancy control. Of 22,367 AIDS cases reported, 3,779 (16.9%) were KS and 741 (3.3%) were NHL. A significant, continuous fall in the percentage of AIDS-KS was seen for both homosexual men and other members of exposure groups during the period 1981-89 (p-trend < 0.0001). The proportion with AIDS-KS decreased from 40.5% in 1983 to 26.5% in 1988 in homosexual men and from 12.2 to 3.6% in other exposure groups, respectively. No significant change was observed in the proportion of NHL cases among any of the risk groups over time, although a tendency towards a slight increase was noted for homosexual men. Comparing proportional trends of KS and NHL geographically, no significant difference was found overall, by time or by exposure group. In conclusion, a specific decline is observed over time for AIDS-KS. However, if geographically-restricted factors are important in the development of non-AIDS-KS in Europe, the same factors do not appear to affect the risk of AIDS-KS. C1 DANISH CANC REGISTRY,INST CANC EPIDEMIOL,DANISH CANC SOC,ROSENVAENGETS HOVEDVEJ 35,BOX 839,DK-2100 COPENHAGEN,DENMARK. GEN CATALUNYA,AIDS EPIDEMIOL SECT,BARCELONA,SPAIN. RIGSHOSP,DEPT INFECT DIS,DK-2100 COPENHAGEN,DENMARK. NCI,VIRAL STUDIES SECT,WASHINGTON,DC. OI Casabona-Barbara, Jordi/0000-0003-4816-5536 NR 28 TC 43 Z9 43 U1 0 U2 0 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD JAN 2 PY 1991 VL 47 IS 1 BP 49 EP 53 DI 10.1002/ijc.2910470110 PG 5 WC Oncology SC Oncology GA ET492 UT WOS:A1991ET49200009 PM 1985878 ER PT J AU HEARING, VJ LEONG, SPL VIEIRA, WD LAW, LW AF HEARING, VJ LEONG, SPL VIEIRA, WD LAW, LW TI SUPPRESSION OF ESTABLISHED PULMONARY METASTASES BY MURINE MELANOMA-SPECIFIC MONOCLONAL-ANTIBODIES SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID ANTIGEN EXPRESSION; TUMOR-GROWTH; CELLS; MICE; LUNG; MODULATION; THERAPY; INVIVO; LIVER AB The intravenous administration of melanoma-specific monoclonal antibodies (MAbs) 9B6 and T97, both of the IgG2b isotype, consistently suppressed the growth of established JB/MS murine melanoma lung metastases. This activity was not dose-dependent, lower doses of MAbs often being more suppressive than higher doses. Intravenous administration of antibodies at days 5 and 8 following challenge appeared to be optimal for suppression whereas no inhibition was seen with intravenous treatment at days 0 and 3 or at days 10 and 13. Consistent and significant inhibition was also observed using established B16F10 lung metastases but only at lower doses, whereas both MAbs were ineffective against the T92497 sarcoma in syngeneic mice. These MAbs appear to act not as direct anti-tumor agents but as host immune response regulators, since specific anti-tumor effects were abrogated in tumor-bearing hosts following pre-treatment with antibodies directed against asialo-GMI and NK-1.1, surface markers of natural killer cells. C1 UNIV ARIZONA,ARIZONA HLTH SCI CTR,ARIZONA CANC CTR,TUCSON,AZ 85724. RP HEARING, VJ (reprint author), NCI,CELL BIOL LAB,BLDG 37,ROOM 1B22,BETHESDA,MD 20892, USA. NR 28 TC 15 Z9 15 U1 0 U2 0 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD JAN 2 PY 1991 VL 47 IS 1 BP 148 EP 153 DI 10.1002/ijc.2910470126 PG 6 WC Oncology SC Oncology GA ET492 UT WOS:A1991ET49200025 PM 1985871 ER PT J AU RAUB, W AF RAUB, W TI GENETIC MUTATION IN FAMILIAL AORTIC-ANEURYSMS IDENTIFIED SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BLDG 31,ROOM 2B23,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JAN 2 PY 1991 VL 265 IS 1 BP 19 EP 19 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EP555 UT WOS:A1991EP55500006 PM 1984118 ER PT J AU RAUB, W AF RAUB, W TI TAMOXIFEN AND CHEMOTHERAPY MORE EFFECTIVE THAN TAMOXIFEN ALONE IN TREATING BREAST-CANCER SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BLDG 31,ROOM 2B23,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JAN 2 PY 1991 VL 265 IS 1 BP 19 EP 19 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EP555 UT WOS:A1991EP55500007 PM 1984118 ER PT J AU RAUB, W AF RAUB, W TI RESEARCH ON EOSINOPHILIA-MYALGIA-SYNDROME SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BLDG 31,ROOM 2B23,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JAN 2 PY 1991 VL 265 IS 1 BP 19 EP 19 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EP555 UT WOS:A1991EP55500008 PM 1984118 ER PT J AU RAUB, W AF RAUB, W TI NEW REPORT ON HYPERTENSION IN PREGNANCY PUBLISHED SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BLDG 31,ROOM 2B23,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JAN 2 PY 1991 VL 265 IS 1 BP 19 EP 19 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EP555 UT WOS:A1991EP55500009 PM 1984118 ER PT J AU MILLS, JL KLEBANOFF, MA GRAUBARD, BI CAREY, JC BERENDES, HW AF MILLS, JL KLEBANOFF, MA GRAUBARD, BI CAREY, JC BERENDES, HW TI BARRIER CONTRACEPTIVE METHODS AND PREECLAMPSIA SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID PRE-ECLAMPSIA AB Recent investigations have suggested that women who use barrier methods of contraception may be at increased risk for preeclampsia. We used data from two prospective pregnancy studies to examine the relationship between contraceptive use before conception and preeclampsia. The preeclampsia rates among women using barrier contraceptives were not significantly higher than the rates in women using nonbarrier contraceptives or the rates in women using no contraceptives in either study. The odds ratios for preeclampsia in barrier contraceptive users in the two studies were 0.89 (95% confidence interval [Cl], 0.71 to 1.12) and 0.85 (95% Cl, 0.49 to 1.45) compared with nonbarrier contraceptive users and 0.91 (95% Cl, 0.71 to 1.16) and 0.81 (95% Cl, 0.48 to 1.35) compared with women using no contraceptives. After adjusting for other risk factors, we found no association between preeclampsia and barrier contraceptive use. Additional studies are needed to resolve this issues; however, we would recommend that women not be advised to avoid barrier contraceptives unless more data linking their use to preeclampsia appear. C1 NCI,BETHESDA,MD 20892. RP MILLS, JL (reprint author), NICHHD,PREVENT RES PROGRAM,EPN BLDG,ROOM 640,BETHESDA,MD 20892, USA. NR 12 TC 21 Z9 21 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JAN 2 PY 1991 VL 265 IS 1 BP 70 EP 73 DI 10.1001/jama.265.1.70 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA EP555 UT WOS:A1991EP55500030 PM 1984127 ER PT J AU BROWN, M AF BROWN, M TI UNITED-STATES MAMMOGRAPHY CAPACITY EXCEEDING USAGE AND NEED SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Editorial Material RP BROWN, M (reprint author), NCI,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD JAN 2 PY 1991 VL 83 IS 1 BP 5 EP 5 DI 10.1093/jnci/83.1.5 PG 1 WC Oncology SC Oncology GA EQ515 UT WOS:A1991EQ51500003 ER PT J AU UNGERLEIDER, RS FRIEDMAN, MA AF UNGERLEIDER, RS FRIEDMAN, MA TI SEX, TRIALS, AND DATATAPES SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article C1 NCI,DIV CANC TREATMENT,CANC THERAPY EVALUAT PROGRAM,BETHESDA,MD 20892. NR 0 TC 9 Z9 9 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD JAN 2 PY 1991 VL 83 IS 1 BP 16 EP 17 DI 10.1093/jnci/83.1.16 PG 2 WC Oncology SC Oncology GA EQ515 UT WOS:A1991EQ51500008 PM 1984514 ER PT J AU CIOCE, V CASTRONOVO, V SHMOOKLER, BM GARBISA, S GRIGIONI, WF LIOTTA, LA SOBEL, ME AF CIOCE, V CASTRONOVO, V SHMOOKLER, BM GARBISA, S GRIGIONI, WF LIOTTA, LA SOBEL, ME TI INCREASED EXPRESSION OF THE LAMININ RECEPTOR IN HUMAN COLON CANCER SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID CARCINOMA-CELLS; TUMOR-CELLS; METASTASIS; ANTIBODY; ATTACHMENT; MEMBRANES; PROTEIN AB It has been proposed that among the various cell-surface proteins capable of interacting with laminin, the 67-kd high-affinity laminin receptor plays a crucial role during tumor invasion and metastasis. In this study, the expression of laminin-receptor-precursor messenger RNA (mRNA) and 67-kd protein was analyzed in human colon adenocarcinoma. In 22 of 23 patients with colon cancer, we found a 2- to 23-fold increase in levels of laminin-receptor-precursor mRNA in the cancer tissues compared with those in matched normal adjacent colonic mucosa. In 10 of 11 cases studied, the level of 67-kd laminin receptor, detected by affinity-purified antilaminin-receptor synthetic peptide antibodies on immunoblots of matched tumor and normal tissue extracts, was higher in the colon carcinoma tissue. Immunodetection of laminin receptor in tissue sections using anti-laminin-receptor-peptide antibodies confirmed that the increased expression of laminin receptor was specifically associated with the cancer cells. In a series of 72 paraffin sections of colon lesions, we observed a correlation between the expression of the laminin receptor and the Dukes' classification. Our observations indicate that increased expression of laminin-receptor-precursor mRNA is associated with enhanced levels of the 67-kd laminin receptor as well as with the invasive phenotype of colon carcinoma. Detection of this metastasis-associated gene product may be a valuable adjunct in the evaluation of human colon cancer. C1 NCI,PATHOL LAB,BLDG 10,RM 2A33,BETHESDA,MD 20892. WASHINGTON HOSP CTR,WASHINGTON,DC 20010. UNIV PADUA,INST HISTOL,I-35100 PADUA,ITALY. UNIV BOLOGNA,INST PATHOL,I-40126 BOLOGNA,ITALY. NR 30 TC 126 Z9 127 U1 0 U2 1 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD JAN 2 PY 1991 VL 83 IS 1 BP 29 EP 36 DI 10.1093/jnci/83.1.29 PG 8 WC Oncology SC Oncology GA EQ515 UT WOS:A1991EQ51500011 PM 1824600 ER PT J AU SMITHGALL, TE JOHNSTON, JB BUSTIN, M GLAZER, RI AF SMITHGALL, TE JOHNSTON, JB BUSTIN, M GLAZER, RI TI ELEVATED EXPRESSION OF THE C-FES PROTOONCOGENE IN ADULT HUMAN MYELOID-LEUKEMIA CELLS IN THE ABSENCE OF GENE AMPLIFICATION SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID V-FES; DIFFERENTIATION; LOCUS; FPS; IDENTIFICATION; SEQUENCES; FAMILY; KINASE AB Expression of the 93-kd tyrosine kinase encoded by the human c-fes proto-on-cogene (also known as FES) is restricted to mature hematopoietic cells of the granulocytic and monocytic lineages, suggestive of a function essential to normal myeloid differentiation. However, recent studies have shown that c-fes can transform fibroblasts if sufficient levels of gene expression are achieved. These findings indicate that strict regulation of the c-fes gene is critical to normal myeloid development, whereas elevated c-fes expression may contribute to malignant transformation. In the present study, we compared the c-fes messenger RNA (mRNA) levels in leukemia blasts from patients with myeloid or lymphoid leukemia with those of peripheral monocytes from a normal donor with the use of a quantitative ribonuclease protection assay. The presence of c-fes mRNA was readily detected in both acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) cells, but c-fes mRNA was present in low levels or was absent in lymphoid leukemia cells. The leukemia cells of two of five AML patients and four of four CML patients expressed more c-fes mRNA than monocytes from a normal donor, with more than a threefold elevation in the cells of one CML patient. No evidence of amplification or rearrangement of the c-fes gene was detectable by Southern blot analysis of myeloid leukemia DNA, suggesting that the variation in c-fes mRNA levels are related to differences in transcriptional activity and/or message stability. These results indicate that elevated c-fes expression is a common feature of myeloid leukemia cells that could potentially contribute to the leukemia phenotype. C1 NCI,BIOL CHEM LAB,BETHESDA,MD 20892. NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. MANITOBA CANC TREATMENT & RES FDN,WINNIPEG,MANITOBA,CANADA. RP SMITHGALL, TE (reprint author), GEORGETOWN UNIV,SCH MED,DEPT PHARMACOL,4 RES CT,ROCKVILLE,MD 20850, USA. RI Bustin, Michael/G-6155-2015 NR 19 TC 12 Z9 12 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD JAN 2 PY 1991 VL 83 IS 1 BP 42 EP 46 DI 10.1093/jnci/83.1.42 PG 5 WC Oncology SC Oncology GA EQ515 UT WOS:A1991EQ51500013 PM 1984516 ER PT J AU CAPORASO, NE AF CAPORASO, NE TI HEREDITY AS A DETERMINING FACTOR IN WHICH SMOKERS DIE OF LUNG-CANCER - RESPONSE SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Letter RP CAPORASO, NE (reprint author), NCI,FAMILY STUDIES SECT,EXECUT PLAZA N,ROOM 439,BETHESDA,MD 20892, USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD JAN 2 PY 1991 VL 83 IS 1 BP 64 EP 65 DI 10.1093/jnci/83.1.64-a PG 2 WC Oncology SC Oncology GA EQ515 UT WOS:A1991EQ51500019 ER PT J AU AGUAYO, LG WEIGHT, FF WHITE, G AF AGUAYO, LG WEIGHT, FF WHITE, G TI TTX-SENSITIVE ACTION-POTENTIALS AND EXCITABILITY OF ADULT-RAT SENSORY NEURONS CULTURED IN SERUM AND EXOGENOUS NERVE GROWTH FACTOR-FREE MEDIUM SO NEUROSCIENCE LETTERS LA English DT Article DE DORSAL ROOT GANGLIA; SERUM-FREE MEDIUM; SENSORY NEURON; TETRODOTOXIN; NERVE GROWTH FACTOR; ACTION POTENTIAL; SODIUM CHANNEL ID ROOT GANGLION NEURONS; SODIUM-CHANNELS; MEMBRANE; ASTROCYTES; SURVIVAL; CURRENTS; CLAMP; CELLS; MOUSE AB The excitability of adult rat dorsal root ganglion (DRG) neurons cultured in the absence of serum and exogenously added nerve growth factor (NGF) was studied. Current-clamp recordings revealed the presence of tetrodotoxin (TTX)-sensitive action potentials. Voltage-clamp recordings demonstrated the presence of both inward and outward currents. The inward Na+ current had a maximal amplitude near - 10 mV and was completely blocked by TTX. A sustained Ca2+ inward current and a slowly activating outward K+ current were also observed. TTX-sensitive and TTX-resistant action potentials have been observed in previous studies in DRG neurons cultured in the presence of serum. By contrast, in the study reported here, only TTX-sensitive action potentials and Na+ currents were found in the neurons cultured in the absence of serum and nerve growth factor. RP AGUAYO, LG (reprint author), NIAAA,PHYSIOL & PHARMACOL STUDIES LAB,ELECTROPHYSIOL SECT,ROCKVILLE,MD 20852, USA. NR 20 TC 11 Z9 11 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD JAN 2 PY 1991 VL 121 IS 1-2 BP 88 EP 92 DI 10.1016/0304-3940(91)90656-E PG 5 WC Neurosciences SC Neurosciences & Neurology GA ET968 UT WOS:A1991ET96800023 PM 2020393 ER PT J AU OTA, M MEFFORD, IN NAOI, M LINNOILA, M AF OTA, M MEFFORD, IN NAOI, M LINNOILA, M TI EFFECTS OF MORPHINE ADMINISTRATION ON CATECHOLAMINE LEVELS IN RAT-BRAIN - SPECIFIC REDUCTION OF EPINEPHRINE CONCENTRATION IN HYPOTHALAMUS SO NEUROSCIENCE LETTERS LA English DT Article DE EPINEPHRINE; MORPHINE-INDUCED DEPLETION; MORPHINE WITHDRAWAL; HYPOTHALAMUS; PHENYLETHANOLAMINE N-METHYLTRANSFERASE; NALOXONE ID NEURONS; CLONIDINE AB The effects of morphine administration on concentrations of epinephrine, norepinephrine and dopamine were examined in the rat brain. Morphine injection reduced the epinephrine level only in the hypothalamus, while the norepinephrine level was reduced in the hypothalamus, medulla, and locus coeruleus. The dopamine concentration was elevated in all regions examined. These changes were blocked by administration of naloxone. Repeated injection of morphine for 14 days did not affect any catecholamine level. In naloxone-induced withdrawal, epinephrine was most markedly depleted in hypothalamus. These observations suggest that the epinephrine level in hypothalamus is affected by morphine acting on opioid receptors. C1 NIAAA,DICBR,CLIN STUDIES LAB,BETHESDA,MD 20892. NIAAA,CLIN SCI LAB,BETHESDA,MD 20892. NAGOYA INST TECHNOL,DEPT BIOSCI,NAGOYA,AICHI 466,JAPAN. NR 16 TC 10 Z9 10 U1 1 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD JAN 2 PY 1991 VL 121 IS 1-2 BP 129 EP 132 DI 10.1016/0304-3940(91)90666-H PG 4 WC Neurosciences SC Neurosciences & Neurology GA ET968 UT WOS:A1991ET96800033 PM 2020369 ER PT J AU KARLSTEN, R POST, C HIDE, I DALY, JW AF KARLSTEN, R POST, C HIDE, I DALY, JW TI THE ANTINOCICEPTIVE EFFECT OF INTRATHECALLY ADMINISTERED ADENOSINE-ANALOGS IN MICE CORRELATES WITH THE AFFINITY FOR THE ADENOSINE-A1-RECEPTOR SO NEUROSCIENCE LETTERS LA English DT Article DE ANTINOCICEPTION; RECEPTOR; XANTHINE ID RAT SPINAL-CORD; SUBREGION; BEHAVIOR AB In the present study, the antinociceptive effects after intrathecal injection of each of 6 N6-substituted adenosine analogs and of 2-phenylaminoadenosine were compared with the affinity for the A1- and A2-adenosine receptors. Adenosine analogs, substituted in the N6-position, had stereoselective structure-dependent antinociceptive effects in the tail flick and hot plate assays after intrathecal injection in mice. The antinociceptive activity for N6-R- and S-phenylisopropyladenosine (R- and S-PIA), N6-R- and S-1-phenyladenosine, N6-1,1-dimethyl-2-phenylethyladenosine (methylPIA), and N6-cyclooctyladenosine correlated with the affinity for central A1-adenosine receptors. An adenosine analog, 2-phenylaminoadenosine, selective for A2-adenosine receptors was inactive in the two tests. These results strongly suggest that spinal A1-adenosine receptors are responsible for the antinociceptive effects of adenosine and its analogs after intrathecal injection. C1 ASTRA RES & DEV LABS,SODERTALJE,SWEDEN. NIDDKD,BIOORGAN CHEM LAB,BETHESDA,MD 20892. RP KARLSTEN, R (reprint author), UNIV HOSP UPPSALA,DEPT ANESTHESIOL,S-75185 UPPSALA,SWEDEN. NR 18 TC 49 Z9 50 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD JAN 2 PY 1991 VL 121 IS 1-2 BP 267 EP 270 DI 10.1016/0304-3940(91)90701-T PG 4 WC Neurosciences SC Neurosciences & Neurology GA ET968 UT WOS:A1991ET96800068 PM 2020383 ER PT B AU HANEY, K AF HANEY, K GP NIST, NATL COMP SECUR CTR TI VIRUSES IN AN OS/2 ENVIRONMENT - REMEMBRANCES OF THINGS PAST AND A HARBINGER OF THINGS TO COME SO 14TH NATIONAL COMPUTER SECURITY CONFERENCE - INFORMATION SYSTEMS SECURITY: REQUIREMENTS & PRACTICES, PROCEEDINGS, VOLS 1 AND 2 LA English DT Proceedings Paper CT 14th National Computer Security Conference - Information Systems Security: Requirements and Practices CY OCT 01-04, 1991 CL WASHINGTON, DC SP NIST, NATL COMP SECUR CTR C1 NIH,DIV COMP RES & TECHNOL,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATL INST STANDARDS & TECHNOLOGY PI BOULDER PA 325 BROADWAY, BOULDER, CO 80303-3328 PY 1991 BP 634 EP 643 PG 10 WC Computer Science, Software Engineering; Computer Science, Theory & Methods SC Computer Science GA BA51M UT WOS:A1991BA51M00063 ER PT J AU ROTH, CA LENFANT, C AF ROTH, CA LENFANT, C TI INCREASING THE NUMBER OF COMPETING AWARDS AT THE NATIONAL HEART, LUNG, AND BLOOD INSTITUTE - PROJECTIONS OF A MODEL SO ACADEMIC MEDICINE LA English DT Article AB In the mid 1980s, the National Heart, Lung, and Blood Institute (NHLBI) sought to extend the benefits of longer award terms to the research community by supporting requests for longer award terms that were scientifically justified. Although investigators welcomed the resultant increase in support stability, concerns were raised about the institute's ability to fund competing awards. A model was developed to assess alternative policies that might result in increased numbers of competing awards. Assuming that the NHLBI receives budget increases sufficient only to keep pace with inflation, the transitory effects of moving to the current policy of longer award terms should largely have passed by 1993. In the long term, the annual number of competing awards will exceed the average number funded between 1980 and 1983. To increase the annual number of competing awards over the long term would require either a reduction in the current percentage of five-year awards or an increase in the total number of active grants. A reduction in the current percentage of five-year awards would subject productive scientists to greater instability in their research support (that is, they would have to apply more frequently for grants) and would introduce greater variability in the number of competing awards available each year. A substantial increase in the total number of active NHLBI awards would be necessary to return the institute's competing awards to the levels of 1984 to 1988. C1 NHLBI,OFF PROGRAM PLANNING & EVALUAT,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 3 TC 0 Z9 0 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 SN 1040-2446 J9 ACAD MED JI Acad. Med. PD JAN PY 1991 VL 66 IS 1 BP 7 EP 13 DI 10.1097/00001888-199101000-00002 PG 7 WC Education, Scientific Disciplines; Health Care Sciences & Services SC Education & Educational Research; Health Care Sciences & Services GA EU045 UT WOS:A1991EU04500002 PM 1985686 ER PT J AU KIRK, KL AF KIRK, KL TI FLUORINE-SUBSTITUTED NEUROACTIVE AMINES SO ACS SYMPOSIUM SERIES LA English DT Review ID BETA-ADRENOCEPTOR ACTIVITIES; DIAZONIUM SALTS; ADRENERGIC-RECEPTORS; RING-FLUORINATION; RAT-BRAIN; PHOTOCHEMISTRY; DOPAMINE; AGENTS; NOREPINEPHRINE; DERIVATIVES AB Fluorine substitution on the aromatic ring of catecholamines has a striking effect on their selectivities for the alpha- and beta-subtypes of adrenergic receptors, with potency at a given receptor dependent on the site of fluorine substitution. The results of testing of new analogs synthesized to probe mechanisms of adrenergic selectivity indicate that a direct effect of the C-F bond on agonist-receptor interaction may be more important than an indirect effect of the C-F bond on the conformation of the ethanolamine side-chain. Research on the effect of fluorine substitution on biological activities of neuroactive amines has been extended to include examination of adrenergic antagonists. RP KIRK, KL (reprint author), NATL INST DIABET & DIGEST & KIDNEY DIS,BIOORGAN CHEM LAB,BETHESDA,MD 20892, USA. NR 31 TC 21 Z9 21 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0097-6156 J9 ACS SYM SER PY 1991 VL 456 BP 136 EP 155 PG 20 WC Chemistry, Multidisciplinary SC Chemistry GA FA936 UT WOS:A1991FA93600009 ER PT J AU JAFFE, H RAINA, AK WAGNER, RM FALES, HM KEMPE, TG KEIM, P BLACHER, RW RILEY, CT AF JAFFE, H RAINA, AK WAGNER, RM FALES, HM KEMPE, TG KEIM, P BLACHER, RW RILEY, CT TI PHEROMONE BIOSYNTHESIS-ACTIVATING NEUROPEPTIDE HORMONE OF HELIOTHIS-ZEA - ISOLATION AND CHARACTERIZATION SO ACS SYMPOSIUM SERIES LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; INSECT NEUROPEPTIDES; CORPORA CARDIACA; BOMBYX-MORI; PURIFICATION; SEQUENCE; PEPTIDES; SILKWORM; DIPTERA AB A pheromone biosynthesis activating neuropeptide hormone (Hez-PBAN) controlling sex pheromone production in moths was isolated from the brain-subesophageal ganglion complexes of the adult corn earworm, Heliothis zea. Hez-PBAN, a 33-residue C-terminally amidated peptide with a molecular weight of 3900, was purified by several high performance liquid and size-exclusion chromatographic steps. Its primary structure was determined by a combination of (1) amino acid analysis, (2) automated Edman degradation, (3) Californium-252 time-of- flight plasma desorption mass spectrometry, and (4) C-terminal sequencing with carboxypeptidase P. Natural Hez-PBAN was found to have undergone oxidation of both its methionines to the corresponding sulfoxides during isolation and purification. Synthetic Hez-PBAN, when oxidized, was shown to exhibit identical chromatographic and spectroscopic properties as the natural material. Synthetic Hez-PBAN at a dose of 2-4 picomoles induced production of a normal quantity of sex pheromone in ligated H. zea females. C1 USDA ARS, BELTSVILLE AGR RES CTR, BELTSVILLE, MD 20705 USA. NIH, BETHESDA, MD 20892 USA. UNIV MARYLAND, COLLEGE PK, MD 20742 USA. APPL BIOSYST INC, FOSTER CITY, CA 94404 USA. JOHNS HOPKINS UNIV, SCH MED, BALTIMORE, MD 21205 USA. NR 19 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0097-6156 J9 ACS SYM SER JI ACS Symp. Ser. PY 1991 VL 453 BP 215 EP 225 PG 11 WC Chemistry, Multidisciplinary SC Chemistry GA EZ325 UT WOS:A1991EZ32500019 ER PT J AU SHIELDS, PG WESTON, A SUGIMURA, H BOWMAN, ED CAPORASO, NE MANCHESTER, DK TRIVERS, GE TAMAI, S RESAU, JH TRUMP, BF HARRIS, CC AF SHIELDS, PG WESTON, A SUGIMURA, H BOWMAN, ED CAPORASO, NE MANCHESTER, DK TRIVERS, GE TAMAI, S RESAU, JH TRUMP, BF HARRIS, CC TI MOLECULAR EPIDEMIOLOGY - DOSIMETRY, SUSCEPTIBILITY, AND CANCER RISK SO ACS SYMPOSIUM SERIES LA English DT Review ID DIOL-EPOXIDE-DNA; FRAGMENT LENGTH POLYMORPHISM; WHITE BLOOD-CELLS; N-ACETYLTRANSFERASE PHENOTYPES; COKE-OVEN WORKERS; L-MYC GENE; LUNG-CANCER; HEMOGLOBIN ADDUCTS; HUMAN-PLACENTA; BLADDER-CANCER AB Molecular epidemiology is a multi-disciplinary field including internal dosimetry assessments of carcinogen exposure, involving metabolic phenotyping and examination of genetic alterations. Carcinogen-adduct studies are becoming increasingly chemically specific and multiple corroborative assays are more frequently performed. These should facilitate interlaboratory comparisons and ultimately improve measurements of biologically effective doses. Metabolic phenotyping can be predictive of cancer risk and available evidence indicates that, when combined with an exposure assessment (PAH, asbestos), predictability is enhanced. Investigations of pediatric tumors have documented that mutation and chromosome loss either inherited or acquired increases cancer risk. Other acquired defects as identified through restriction fragment length polymorphism analysis for HRAS-1 and MYCL relates to tumor incidence and metastasis. Analyses of lung cancer cases demonstrate that loss of heterozygosity of allelic DNA sequences in non-random in both small-cell and non-small-cell lung cancer. This paper will review our experience in molecular epidemiology as well as summarize significant accomplishments of other investigators. RP SHIELDS, PG (reprint author), NCI,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. RI Shields, Peter/I-1644-2012 NR 131 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0097-6156 J9 ACS SYM SER PY 1991 VL 451 BP 186 EP 206 PG 21 WC Chemistry, Multidisciplinary SC Chemistry GA EU894 UT WOS:A1991EU89400017 ER PT J AU STRICKLAND, PT ROTHMAN, N BASER, ME POIRIER, MC AF STRICKLAND, PT ROTHMAN, N BASER, ME POIRIER, MC TI POLYCYCLIC AROMATIC HYDROCARBON DNA ADDUCT LOAD IN PERIPHERAL-BLOOD CELLS - CONTRIBUTION OF MULTIPLE EXPOSURE SOURCES SO ACS SYMPOSIUM SERIES LA English DT Article ID SISTER CHROMATID EXCHANGE; METABOLISM; NONSMOKERS; SMOKERS; REPAIR AB Polycyclic aromatic hydrocarbons (PAH) are produced by combustion of organic materials, and humans are exposed to these compounds from a variety of sources. In a recent cross-sectional study of PAH exposure in residential fire fighters and controls, PAH-DNA adducts in peripheral nucleated blood cells were examined by immunoassay (ELISA) as potential markers of exposure. Mean adduct levels in caucasian participants (22 detectable/66 tested) increased with exposure to one or more sources of PAH: fire fighting, smoking, and/or char-broiled (CB) food consumption. The effect of dietary PAH on PAH-DNA adduct levels was further investigated in a controlled exposure study in which 4 volunteers consumed CB beef daily for 7 days. PAH-DNA adduct levels increase 3-fold and 6-fold above baseline levels and in two individuals and remained unchanged in two individuals during the feeding period. These results suggest that multiple sources of PAH contribute to the PAH-DNA adduct load in peripheral blood cells and confirm the importance of dietary contributions. C1 JOHNS HOPKINS UNIV,SCH PUBL HLTH,DEPT EPIDEMIOL,BALTIMORE,MD 21205. NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892. RP STRICKLAND, PT (reprint author), JOHNS HOPKINS UNIV,SCH PUBL HLTH,DEPT ENVIRONM HLTH SCI,BALTIMORE,MD 21205, USA. NR 14 TC 3 Z9 3 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0097-6156 J9 ACS SYM SER PY 1991 VL 451 BP 257 EP 263 PG 7 WC Chemistry, Multidisciplinary SC Chemistry GA EU894 UT WOS:A1991EU89400022 ER PT J AU POIRIER, MC GUPTABURT, S LITTERST, CL REED, E AF POIRIER, MC GUPTABURT, S LITTERST, CL REED, E TI DETECTION OF CISPLATIN DNA ADDUCTS IN HUMANS SO ACS SYMPOSIUM SERIES LA English DT Article ID CANCER-PATIENTS; QUANTITATION; CHEMOTHERAPY AB An ELISA, which measures cisplatin-DNA intrastrand adducts, and atomic absorbance spectrometry, which measures total platinum bound to DNA, have been used to quantify DNA modification in samples from patients receiving platinum drug-based therapy and rats in which the treatment of human cancer patients has been modeled. Adducts measured in blood cell DNA samples from cancer patients have correlated with dose and chemotherapeutic efficacy. Human tissue DNA adducts have a widespread distribution, and long-term adduct persistence ( > 1 year) has been observed in many organs including tumor and target sites for drug toxicity. C1 NIAID,BETHESDA,MD 20892. RP POIRIER, MC (reprint author), NCI,BETHESDA,MD 20892, USA. NR 12 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0097-6156 J9 ACS SYM SER PY 1991 VL 451 BP 300 EP 307 PG 8 WC Chemistry, Multidisciplinary SC Chemistry GA EU894 UT WOS:A1991EU89400027 ER PT J AU BLUM, MS EVERETT, DM JONES, TH FALES, HM AF BLUM, MS EVERETT, DM JONES, TH FALES, HM TI ARTHROPOD NATURAL-PRODUCTS AS INSECT REPELLENTS SO ACS SYMPOSIUM SERIES LA English DT Review ID APIS-MELLIFERA L; THRIPS THYSANOPTERA; THIEF ANT; SECRETION; PHLAEOTHRIPIDAE; IDENTIFICATION; VENOM; SOLENOPSIS; PHEROMONE; AMINOACETOPHENONE AB The defensive allomones of arthropods have been evolved to blunt the attacks of a variety of predatory species. These natural products function primarily by repelling adversaries and thus usually constitute the first line of defense. An examination of the deterrent efficacies of compounds produced by honey bees, thrips, and ants demonstrates that these exocrine products are highly effective repellents against a diversity of ant species. A potpourri of natural products including aliphatic and aromatic ketones, esters, fatty acids, and alkaloids has been determined to possess well-developed repellent properties at physiological concentrations. These results emphasize the great potential of insect-derived compounds as an outstanding source of repellents in the never-ending battle with species of pest arthropods. C1 UNIV GEORGIA, DEPT COMP SCI, ATHENS, GA 30602 USA. NHLBI, CHEM LAB, BETHESDA, MD 20892 USA. RP BLUM, MS (reprint author), UNIV GEORGIA, DEPT ENTOMOL, ATHENS, GA 30602 USA. NR 45 TC 0 Z9 0 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0097-6156 J9 ACS SYM SER JI ACS Symp. Ser. PY 1991 VL 449 BP 14 EP 26 PG 13 WC Chemistry, Multidisciplinary SC Chemistry GA EW910 UT WOS:A1991EW91000002 ER PT J AU MAGUIRE, NC DROZD, K LUFF, RD KANTOR, RRS AF MAGUIRE, NC DROZD, K LUFF, RD KANTOR, RRS TI IMMUNOHISTOCHEMICAL LOCALIZATION OF GLUTATHIONE-S-TRANSFERASE IN PRENEOPLASTIC AND NEOPLASTIC LESIONS OF THE HUMAN UTERINE CERVIX SO ACTA CYTOLOGICA LA English DT Article ID HUMAN PAPILLOMAVIRUS INFECTION; SQUAMOUS-CELL CARCINOMA; PLACENTAL FORM; CARCINOEMBRYONIC ANTIGEN; HEPATOCARCINOGENESIS; CARCINOGENESIS; PROTEIN; MARKERS; CEA AB A mouse monoclonal antibody and a rabbit polyclonal antibody prepared against the placental form of the enzyme glutathione S-transferase (GST-pi) were used to immunohistochemically stain normal and neoplastic human utrine cervical tissues from 88 cases. Of 65 cases of preneoplastic squamous lesions and invasive carcinomas of the cervix, 94% stained with the monoclonal antibody and 100% with the polyclonal antibody. In the 23 benign tissues, staining of ectocervical squamous epithelium was generally not observed; however, areas of reserve-cell hyperplasia, immature squamous metaplasia and adjacent endocervical cells did show staining (68% with the monoclonal antibody and 95% with the polyclonal antibody). Many of the positive tissue types showed a variety of staining patterns and intensities. These findings do not support the concept that GST-pi staining can be used to distinguish preneoplastic lesions of the cervix from benign reactive or proliferative processes. These results are of interest in the investigation of cervical carcinogenesis since GST-pi may be involved in an early stage of neoplastic transformation of the cervical epithelium. The correlation of these findings with the results of human papillomavirus testing and DNA content analysis should be of interest in determining the relationship of this enzyme to cervical neoplasia. C1 SACRED HEART HOSP,DEPT PATHOL,ALLENTOWN,PA. NCI,FREDERICK CANC RES CTR,PROGRAM RESOURCES INC,FREDERICK,MD 21701. RP MAGUIRE, NC (reprint author), THOMAS JEFFERSON UNIV,COLL ALLIED HLTH SCI,DEPT LAB SCI,130 S 9TH ST,ROOM 1924,PHILADELPHIA,PA 19107, USA. FU NCI NIH HHS [N01-CO-74102] NR 20 TC 10 Z9 10 U1 0 U2 0 PU SCI PRINTERS & PUBL INC PI ST LOUIS PA P.O. DRAWER 12425 8342 OLIVE BLVD, ST LOUIS, MO 63132 SN 0001-5547 J9 ACTA CYTOL JI Acta Cytol. PD JAN-FEB PY 1991 VL 35 IS 1 BP 94 EP 99 PG 6 WC Pathology SC Pathology GA EX432 UT WOS:A1991EX43200018 PM 1994644 ER PT J AU HU, YH PAN, XR LIU, PA LI, GW HOWARD, BV BENNETT, PH AF HU, YH PAN, XR LIU, PA LI, GW HOWARD, BV BENNETT, PH TI CORONARY HEART-DISEASE AND DIABETIC-RETINOPATHY IN NEWLY DIAGNOSED DIABETES IN DA-QING, CHINA - THE DA-QING IGT AND DIABETES STUDY SO ACTA DIABETOLOGICA LA English DT Article DE CORONARY HEART DISEASE; DIABETIC RETINOPATHY AB In 1986, 110,660 of 281,589 residents aged 25-74 years in Da Qing, Hei Long Jiang Province of China, were surveyed. Based on the results of a 75-g oral glucose tolerance test, 630 subjects were found to have previously undiagnosed diabetes according to 1985 WHO criteria. Among them, 600 diabetics aged 35-74 years (288 men, 312 women) and 410 non-diabetics of similar age with normal glucose tolerance (207 men, 203 women) were examined to determine the prevalence of retinopathy and coronary heart disease (CHD) and to evaluate associated characteristics. Retinal examinations of 423 newly diagnosed diabetics showed that 15.4% had several microaneurysms and/or small intraretinal haemorrhage, 5.5% soft exudates, 7.1 % hard exudates, and 2.3 % proliferative retinopathy. Among 220 non-diabetics, 13.6% had one or two microaneurysms and/or small intraretinal haemorrhage, and only 1.4% had a few soft exudates; half of the non-diabetics with retinopathy had hypertension. CHD, according to Minnesota coding (1.1-1.3, 5.1-5.3 and 7.1) of resting electrocardiograms, was ten times more frequent in the diabetics (3.59%) than in the controls (0.32%), after adjusting for age and sex. Multiple regression analysis showed that plasma glucose concentration was a risk factor for retinopathy after adjusting for age, sex, body mass index (BMI), smoking and blood pressure. Two-hour plasma glucose concentration (after adjusting for age, sex, BMI, smoking and blood pressure) and blood pressure (after adjusting for age, sex BMI, smoking and 1-h or 2-h plasma glucose level) were associated with CHD among the diabetics and non-diabetics and among the diabetics alone. Thus, both micro-and macrovascular complications occur frequently in previously undiagnosed Chinese diabetics and the frequency of CHD is markedly increased compared to the low frequency among Chinese non-diabetics. C1 DA QING FIRST HOSP,DA QING,PEOPLES R CHINA. CHINA JAPAN FRIENDSHIP HOSP,DEPT ENDOCRINOL,BEIJING 100029,PEOPLES R CHINA. MEDLANTIC RES FDN,WASHINGTON,DC. NIDDKD,PHOENIX EPIDEMIOL & CLIN RES BRANCH,PHOENIX,AZ. NR 16 TC 17 Z9 17 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0940-5429 J9 ACTA DIABETOL JI Acta Diabetol. PY 1991 VL 28 IS 2 BP 169 EP 173 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA GQ144 UT WOS:A1991GQ14400007 PM 1777654 ER PT J AU STRONG, MJ GARRUTO, RM WOLFF, AV CHOU, SM FOX, SD YANAGIHARA, R AF STRONG, MJ GARRUTO, RM WOLFF, AV CHOU, SM FOX, SD YANAGIHARA, R TI N-BUTYL BENZENESULFONAMIDE - A NEUROTOXIC PLASTICIZER INDUCING A SPASTIC MYELOPATHY IN RABBITS SO ACTA NEUROPATHOLOGICA LA English DT Article DE EXPERIMENTAL MYELOPATHY; NEUROAXONAL DEGENERATION; ORGANOTOXIN; POLYMERIZING AGENT ID BLOOD BAGS AB N-Butyl benzenesulfonamide (NBBS), a plasticizer used commercially in the polymerization of polyamide compounds, is neurotoxic. Young adult New Zealand white rabbits, inoculated repeatedly with NBBS by the intracisternal or intraperitoneal routes, developed a dose-dependent motor dysfunction characterized by limb splaying, hyperreflexia, hypertonia, gait impairment, and abnormal righting reflexes. Histopathological changes consisted of intramedullary thickening of the ventral horn axons, random neuroaxonal spheroids confined to brain stem nuclei and spinal motor neurons, and swollen dendritic processes of spinal motor neurons. Immunoreactivity to a monoclonal antibody against microtubule-associated protein-2 (MAP-2) was markedly increased in the dendrites of spinal motor neurons following thrice weekly intraperitoneal inoculations of NBBS for 4 months, whereas after 12 monthly intracisternal inoculations, MAP-2 immunoreactivity was absent or strikingly reduced in the same neuronal populations. Ultrastructurally, postsynaptic zones contained vacuoles and multilamellar bodies. These findings raise questions about the safety of NBBS to humans. C1 NINCDS,CENT NERVOUS SYST STUDIES LAB,BETHESDA,MD 20892. CLEVELAND CLIN EDUC FDN,DEPT PATHOL,CLEVELAND,OH 44106. PROGRAM RESOURCES INC,FREDERICK CANC RES & DEV CTR,CHEM SYNTH & ANAL LAB,FREDERICK,MD 21702. RI Strong, Michael/H-9689-2012 NR 18 TC 22 Z9 22 U1 0 U2 4 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0001-6322 J9 ACTA NEUROPATHOL JI Acta Neuropathol. PY 1991 VL 81 IS 3 BP 235 EP 241 PG 7 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA EY496 UT WOS:A1991EY49600001 PM 2058361 ER PT J AU KOISTINAHO, J WADHWANI, KC BALBO, A RAPOPORT, SI AF KOISTINAHO, J WADHWANI, KC BALBO, A RAPOPORT, SI TI REGENERATION OF PERIVASCULAR ADRENERGIC-INNERVATION IN RAT TIBIAL NERVE AFTER NERVE CRUSH SO ACTA NEUROPATHOLOGICA LA English DT Article DE TIBIAL NERVE; REGENERATION; ADRENERGIC INNERVATION; ENDONEURIUM; EPI-PERINEURIUM ID BLOOD-VESSELS; NEOVASCULARIZATION; RABBIT; INJURY AB Adrenergic innervation of blood vessels in the rat tibial nerve during degeneration and regeneration was studied using the formaldehyde-induced fluorescence method. The left sciatic nerve was crushed with suture threads to produce a 4-mm length of crushed nerve. At 1, 3, 7, 14, 28, 56 and 84 days after nerve crush. degenerative and regenerative changes in the nerve were verified using light microscopy. At each time point, adrenergic innervation was examined in epi-perineurial whole mount and nerve cross-section preparations. One day after nerve crush, fluorescence of adrenergic nerve fibers in the endoneurium was absent. Fluorescent adrenergic nerve fibers reappeared in the endoneurium at day 56 and reached the control density by 84 days. In the epi-perineurium, adrenergic innervation of small and medium-size arterioles was absent at 3 days, in large arterioles at 7 days. At 56 days, all epi-perineurial arterioles were reinnervated by a faint, sparse adrenergic network, which reached the control density at 84 days. The results suggest that adrenergic innervation in the rat peripheral nerve is lost during nerve degeneration, but recovers when the nerve has regenerated. C1 NIA,NEUROSCI LAB,BETHESDA,MD 20892. NR 25 TC 3 Z9 3 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0001-6322 J9 ACTA NEUROPATHOL JI Acta Neuropathol. PY 1991 VL 81 IS 5 BP 486 EP 490 PG 5 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA FH937 UT WOS:A1991FH93700003 PM 1713392 ER PT J AU POWELL, HC GARRETT, RS KADOR, PF MIZISIN, AP AF POWELL, HC GARRETT, RS KADOR, PF MIZISIN, AP TI FINE-STRUCTURAL LOCALIZATION OF ALDOSE REDUCTASE AND OUABAIN-SENSITIVE, K+-DEPENDENT PARA-NITRO-PHENYLPHOSPHATASE IN RAT PERIPHERAL-NERVE SO ACTA NEUROPATHOLOGICA LA English DT Article ID POTASSIUM ADENOSINE-TRIPHOSPHATASE; ENDONEURIAL FLUID SODIUM; CYTOCHEMICAL-LOCALIZATION; GALACTOSE INTOXICATION; CHLORIDE ACCUMULATION; DOSE-DEPENDENCE; PHOSPHATASE; BARRIER; SYSTEMS; ENZYME AB Aldose reductase was visualized by light and electron microscopy using a goat anti-rat antibody with immunoperoxidase and immunogold, respectively. Oua-bain-sensitive, K+-dependent, p-nitro-phenylphosphatase, a component of (Na+, K+)-ATPase, was localized at the electron microscopic level by enzyme histochemistry using p-nitro-phenylphosphate as substrate. In peripheral nerve, spinal ganglia and roots, the Schwann cell of myelinated fibers was the principal site of aldose reductase localization. Immunostaining was intense in the paranodal region and the Schmidt-Lanterman clefts as well as in cytoplasm of the terminal expansions of paranodal myelin lamellae and the nodal microvilli. Schwann cell cytoplasm of unmyelinated fibers were faintly labelled. Endoneurial vessel endothelia, pericytes and perineurium failed to bind appreciable amounts of aldose reductase antibody. However, mast cell granules bound antibody strongly. In contrast, p-nitro-phenylphosphatase reaction product was detected in the nodal axolemma, terminal loops of Schwann cell cytoplasm and the innermost layer of perineurial cells. In endothelial cells, reaction product was localized on either the luminal or abluminal, or on both luminal and abluminal plasmalemma. Endothelial vesicular profiles were often loaded with reaction product. Occasional staining of myelin and axonal organelles was noted. Mast cells lacked reaction product. C1 VET ADM MED CTR,SAN DIEGO,CA 92161. NEI,BETHESDA,MD 20892. RP POWELL, HC (reprint author), UNIV CALIF SAN DIEGO,DEPT PATHOL NEUROPATHOL,LA JOLLA,CA 92093, USA. FU PHS HHS [18715, 14162] NR 27 TC 64 Z9 64 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0001-6322 J9 ACTA NEUROPATHOL JI Acta Neuropathol. PY 1991 VL 81 IS 5 BP 529 EP 539 PG 11 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA FH937 UT WOS:A1991FH93700010 PM 1650113 ER PT J AU TARUSCIO, D ALBEDI, FM BAGNATO, R PAULUZZI, S FRANCISCI, D CAVALIERE, A DONELLI, G AF TARUSCIO, D ALBEDI, FM BAGNATO, R PAULUZZI, S FRANCISCI, D CAVALIERE, A DONELLI, G TI INCREASED REACTIVITY OF LAMININ IN THE BASEMENT-MEMBRANES OF CAPILLARY WALLS IN AIDS BRAIN CORTEX SO ACTA NEUROPATHOLOGICA LA English DT Article DE ACQUIRED IMMUNODEFICIENCY SYNDROME; BRAIN CORTEX; CAPILLARY BASEMENT MEMBRANE; LAMININ; COLLAGEN-IV ID HETEROGENEITY; LOCALIZATION; ASTROCYTES; INFECTION; TISSUE AB To verify how the components of the capillary wall are modified in the course of AIDS we studied the brain cortex from nine cases with AIDS. Cellular and extracellular components were delineated using antibodies for laminin and collagen IV for basement membranes and glial fibrillary acidic protein for astrocyte foot processes. We found a marked increase in reactivity for laminin in the basement membranes of capillary walls and hypertrophy and hyperplasia of astrocyte foot processes around vessels, when compared to control cortical tissue. We suggest that modifications of brain capillary wall may have a role in the pathogenesis of neurological disfunction in AIDS. C1 NIH,DEPT ULTRASTRUCT,VIALE REGINA ELENA 299,BETHESDA,MD 20892. UNIV PERUGIA,DEPT INFECT DIS,I-06100 PERUGIA,ITALY. UNIV PERUGIA,DEPT PATHOL,I-06100 PERUGIA,ITALY. RI TARUSCIO, DOMENICA/A-6646-2015; Malchiodi-Albedi, Fiorella/A-5944-2015 OI TARUSCIO, DOMENICA/0000-0001-5403-233X; Malchiodi-Albedi, Fiorella/0000-0001-7202-0391 NR 13 TC 8 Z9 8 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0001-6322 J9 ACTA NEUROPATHOL JI Acta Neuropathol. PY 1991 VL 81 IS 5 BP 552 EP 556 PG 5 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA FH937 UT WOS:A1991FH93700013 PM 1713397 ER PT J AU MERRIAM, GR WACHTER, KW MA, N LIU, L AF MERRIAM, GR WACHTER, KW MA, N LIU, L TI SYNCHRONIZATION OF HORMONAL TIME-SERIES - ANALYTICAL APPROACHES SO ACTA PAEDIATRICA SCANDINAVICA LA English DT Article; Proceedings Paper CT 10TH INTERNATIONAL SYMPOSIUM ON GROWTH AND GROWTH DISORDERS CY OCT 19-20, 1990 CL MADRID, SPAIN SP KABI PHARM DE PULSES; HORMONES; SIMULTANEITY; COINCIDENCE; LUTEINIZING HORMONE; PROLACTIN AB Comparison of the patterns of episodic secretion of two or more hormones measured simultaneously of ten suggests that peak levels tend to occur at around the same time. This visual impression can be misleading, however, as some peaks will occur simultaneously due to chance alone. Traditional cross-correlation methods are suitable for evaluating linkages among circadian and other smoothly varying rhythms, but are less well suited to correlating ultradian peaks. This paper proposes a method which entails identifying peaks of hormone secretion as discrete events in several different series, and counting the frequency of simultaneous and near-simultaneous peaks in temporal 'windows' of increasing width. The expected rates of random coincidences can be assessed by a variety of methods, including simulations and probability calculations. The method described utilizes simple techniques which make few assumptions about the character or stationarity of the series, comparing coincidence rates in the appropriately paired series with those in which a series in one subject is deliberately 'mismatched' with that of another subject. Using this approach, a significant excess of simultaneous peaks of luteinizing hormone (LH) and prolactin was found in normal subjects as compared with mismatched series (17% versus 6%; p < 0.05). Hypogonadotrophic men treated with pulsatile gonadotrophin-releasing hormone (GnRH) have an even higher coincidence rate (26%). By contrast, the rate of coincidence between pulses of LH and cortisol is not significantly greater than that which would be expected due to chance. C1 UNIV CALIF BERKELEY,DEPT STAT,BERKELEY,CA 94720. RP MERRIAM, GR (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,BLDG 10,ROOM 10N262,BETHESDA,MD 20892, USA. NR 8 TC 0 Z9 0 U1 0 U2 0 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0001-656X J9 ACTA PAEDIATR SCAND PY 1991 SU 372 BP 63 EP 68 PG 6 WC Pediatrics SC Pediatrics GA FP053 UT WOS:A1991FP05300011 ER PT J AU ROTEVATN, S SARHEIM, H MURPHY, E AF ROTEVATN, S SARHEIM, H MURPHY, E TI INTRACELLULAR FREE MAGNESIUM CONCENTRATION - RELEVANCE TO CARDIOVASCULAR MEDICINE SO ACTA PHYSIOLOGICA SCANDINAVICA LA English DT Article ID CYTOSOLIC FREE MAGNESIUM; ISCHEMIC RAT-HEART; GUINEA-PIG; CARDIAC MYOCYTES; FLUORESCENT INDICATOR; VENTRICULAR CELLS; POTASSIUM CHANNEL; ION CONCENTRATION; CALCIUM CURRENT; MUSCLE-FIBERS AB There is a growing awareness of the role hypomagnesemia plays in cardiovascular medicine. Recent experimental studies have also provided a new understanding og how Mg2+ ions influence various ion channels and transport mechanisms. Yet, the pathophysiological mechanisms that may be responsible for various phenomena associated with hypomagnesemia, have not been clarified. This is partly due to that there, until recently, has been a lack of convenient and reliable methods for measuring cytosolic free Mg2+ concentration. It is the hope that newly developed techniques for measuring the cytosolic free Mg2+ concentration will prove useful in this respect. C1 NIEHS, MOLEC BIOPHYS LAB, RES TRIANGLE PK, NC 27709 USA. RP UNIV BERGEN, DEPT ANAT, ARSTADVEIEN 19, N-5009 BERGEN, NORWAY. NR 37 TC 4 Z9 4 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0001-6772 J9 ACTA PHYSIOL SCAND JI Acta Physiol. Scand. PY 1991 VL 142 SU 599 BP 125 EP 133 PG 9 WC Physiology SC Physiology GA FQ911 UT WOS:A1991FQ91100013 ER PT J AU NEWLIN, DB PRETORIUS, MB AF NEWLIN, DB PRETORIUS, MB TI NONASSOCIATIVE MECHANISMS IN PREFERENCES FOR ALCOHOLIC FLAVORS - DIFFERENCES BETWEEN SONS OF ALCOHOLICS AND SONS OF NONALCOHOLICS SO ADDICTIVE BEHAVIORS LA English DT Article ID EXPOSURE; GENETICS AB We studied nonassociative processes in the development of preferences for wine flavors in high- and low-risk groups for alcoholism. Twenty college-age sons of nonalcoholics had greater "Liking" ratings for red wine flavors to which they had 20 prior exposures in the laboratory than flavors to which they had been exposed five times or zero times. Fourteen sons of alcoholics did not show this mere exposure effect. The results indicate differences between high- and low-risk groups for alcoholism in the nonassociative process by which preference for particular alcoholic flavors is established. RP NEWLIN, DB (reprint author), NIDA,ADDICT RES CTR,BOX 5180,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. FU NIAAA NIH HHS [AA06433] NR 15 TC 3 Z9 3 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4603 J9 ADDICT BEHAV JI Addict. Behav. PY 1991 VL 16 IS 6 BP 481 EP 487 DI 10.1016/0306-4603(91)90055-M PG 7 WC Psychology, Clinical; Substance Abuse SC Psychology; Substance Abuse GA GV796 UT WOS:A1991GV79600010 PM 1801571 ER PT J AU WINDLE, C AF WINDLE, C TI MENTAL-HEALTH-SERVICES RESEARCH - WHAT MENTAL-HEALTH-SERVICES RESEARCH DOES NIMH SUPPORT SO ADMINISTRATION AND POLICY IN MENTAL HEALTH LA English DT Article ID HOSPITALIZATION; PSYCHIATRY; INSURANCE; ILLNESS; CARE RP WINDLE, C (reprint author), NIMH,DIV BIOMETRY & APPL SCI,ROCKVILLE,MD 20857, USA. NR 26 TC 3 Z9 3 U1 0 U2 0 PU HUMAN SCI PRESS INC PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013-1578 SN 0894-587X J9 ADM POLICY MENT HLTH JI Adm. Policy. Ment. Health PD JAN PY 1991 VL 18 IS 3 BP 199 EP 203 DI 10.1007/BF00713805 PG 5 WC Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA EU900 UT WOS:A1991EU90000007 ER PT J AU BOLEN, JB THOMPSON, PA EISEMAN, E HORAK, ID AF BOLEN, JB THOMPSON, PA EISEMAN, E HORAK, ID TI EXPRESSION AND INTERACTIONS OF THE SRC FAMILY OF TYROSINE PROTEIN-KINASES IN LYMPHOCYTES-T SO ADVANCES IN CANCER RESEARCH LA English DT Review ID ROUS-SARCOMA VIRUS; CELL ANTIGEN RECEPTOR; HUMAN INTERLEUKIN-2 RECEPTOR; AMINO-TERMINAL DOMAIN; TISSUE-SPECIFIC EXPRESSION; SITE-DIRECTED MUTAGENESIS; 5' UNTRANSLATED REGIONS; MEMBRANE-BOUND FORMS; PDGF BETA-RECEPTOR; 2 LCK TRANSCRIPTS C1 NCI,CLIN PHARMACOL BRANCH,BETHESDA,MD 20892. RP BOLEN, JB (reprint author), NCI,TUMOR VIRUS BIOL LAB,BETHESDA,MD 20892, USA. NR 275 TC 87 Z9 87 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0065-230X J9 ADV CANCER RES JI Adv. Cancer Res. PY 1991 VL 57 BP 103 EP 149 DI 10.1016/S0065-230X(08)60997-5 PG 47 WC Oncology SC Oncology GA GN460 UT WOS:A1991GN46000004 PM 1950702 ER PT J AU MILES, EW AF MILES, EW TI STRUCTURAL BASIS FOR CATALYSIS BY TRYPTOPHAN SYNTHASE SO ADVANCES IN ENZYMOLOGY AND RELATED AREAS OF MOLECULAR BIOLOGY LA English DT Review ID ULTRAVIOLET VISIBLE SPECTROSCOPY; SYNTHETASE BETA-2 SUBUNIT; SITE-SPECIFIC MUTAGENESIS; D-SERINE DEHYDRATASE; AMINO-ACID SEQUENCE; OXINDOLYL-L-ALANINE; ESCHERICHIA-COLI; ALPHA-SUBUNIT; SALMONELLA-TYPHIMURIUM; PYRIDOXAL 5'-PHOSPHATE RP MILES, EW (reprint author), NIH, BIOCHEM & PHARMACOL LAB, BETHESDA, MD 20892 USA. NR 179 TC 2 Z9 2 U1 1 U2 3 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0065-258X J9 ADV ENZYMOL RAMB JI Adv. Enzymol. Relat. Areas Mol. Biol. PY 1991 VL 64 BP 93 EP + PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FG007 UT WOS:A1991FG00700003 ER PT J AU THOMPSON, J MILLER, SPF AF THOMPSON, J MILLER, SPF TI N-5-(1-CARBOXYETHYL)ORNITHINE AND RELATED [N-CARBOXYALKYL]-AMINO ACIDS - STRUCTURE, BIOSYNTHESIS, AND FUNCTION SO ADVANCES IN ENZYMOLOGY AND RELATED AREAS OF MOLECULAR BIOLOGY LA English DT Review ID CROWN-GALL TUMORS; LYSINE-KETOGLUTARATE REDUCTASE; N-EPSILON-CARBOXYMETHYLLYSINE; ARGININE DEIMINASE PATHWAY; AMINOADIPIC SEMIALDEHYDE SYNTHASE; GAS-LIQUID-CHROMATOGRAPHY; AGROBACTERIUM TI-PLASMID; TISSUE-SPECIFIC ISOZYMES; DNA-ENCODED TRANSCRIPTS; FREE AMINO-ACIDS C1 NINCDS, DEV & METAB NEUROL BRANCH, BETHESDA, MD 20892 USA. RP THOMPSON, J (reprint author), NIDR, MICROBIAL ECOL LAB, BETHESDA, MD 20892 USA. NR 307 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0065-258X J9 ADV ENZYMOL RAMB JI Adv. Enzymol. Relat. Areas Mol. Biol. PY 1991 VL 64 BP 317 EP 399 PG 83 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FG007 UT WOS:A1991FG00700007 ER PT B AU ROSOLEN, A WHITESELL, L IKEGAKI, N KENNETT, R NECKERS, LM AF ROSOLEN, A WHITESELL, L IKEGAKI, N KENNETT, R NECKERS, LM BE EVANS, AE DANGIO, GJ KNUDSON, AG SEEGER, RC TI ANTISENSE INHIBITION OF N-MYC REDUCES CELL-GROWTH BUT DOES NOT AFFECT C-MYC EXPRESSION IN THE NEUROEPITHELIOMA CELL-LINE CHP100 SO ADVANCES IN NEUROBLASTOMA RESEARCH 3 SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT 5TH SYMP ON ADVANCES IN NEUROBLASTOMA RESEARCH CY MAY 28-30, 1990 CL CHILDRENS HOSP, PHILADELPHIA, PA HO CHILDRENS HOSP RP ROSOLEN, A (reprint author), NCI,MED BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56085-5 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 366 BP 29 EP 36 PG 8 WC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics SC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics GA BT23Z UT WOS:A1991BT23Z00004 PM 2068146 ER PT B AU WHITESELL, L ROSOLEN, A NECKERS, LM AF WHITESELL, L ROSOLEN, A NECKERS, LM BE EVANS, AE DANGIO, GJ KNUDSON, AG SEEGER, RC TI ANTISENSE SUPPRESSION OF N-MYC EXPRESSION INHIBITS THE TRANSDIFFERENTIATION OF NEUROECTODERM TUMOR-CELL LINES SO ADVANCES IN NEUROBLASTOMA RESEARCH 3 SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT 5TH SYMP ON ADVANCES IN NEUROBLASTOMA RESEARCH CY MAY 28-30, 1990 CL CHILDRENS HOSP, PHILADELPHIA, PA HO CHILDRENS HOSP RP WHITESELL, L (reprint author), NCI,MED BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 4 Z9 4 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56085-5 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 366 BP 45 EP 54 PG 10 WC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics SC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics GA BT23Z UT WOS:A1991BT23Z00006 PM 2068159 ER PT B AU THIELE, CJ AF THIELE, CJ BE EVANS, AE DANGIO, GJ KNUDSON, AG SEEGER, RC TI PATTERNS OF REGULATION OF NUCLEAR PROTOONCOGENES MYCN AND MYB IN RETINOIC ACID-TREATED NEUROBLASTOMA-CELLS SO ADVANCES IN NEUROBLASTOMA RESEARCH 3 SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT 5TH SYMP ON ADVANCES IN NEUROBLASTOMA RESEARCH CY MAY 28-30, 1990 CL CHILDRENS HOSP, PHILADELPHIA, PA HO CHILDRENS HOSP RP THIELE, CJ (reprint author), NCI,MOLEC GENET SECT,BETHESDA,MD 20892, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56085-5 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 366 BP 151 EP 156 PG 6 WC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics SC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics GA BT23Z UT WOS:A1991BT23Z00017 PM 2068135 ER PT B AU GAETANO, C MATSUMOTO, K THIELE, CJ AF GAETANO, C MATSUMOTO, K THIELE, CJ BE EVANS, AE DANGIO, GJ KNUDSON, AG SEEGER, RC TI RETINOIC ACID RESISTANT NEUROBLASTOMA-CELLS AND THE EXPRESSION OF INSULIN-LIKE GROWTH FACTOR-II SO ADVANCES IN NEUROBLASTOMA RESEARCH 3 SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT 5TH SYMP ON ADVANCES IN NEUROBLASTOMA RESEARCH CY MAY 28-30, 1990 CL CHILDRENS HOSP, PHILADELPHIA, PA HO CHILDRENS HOSP RP GAETANO, C (reprint author), NCI,PEDIAT BRANCH,MOLEC GENET SECT,BETHESDA,MD 20892, USA. OI Gaetano, Carlo/0000-0002-5238-1832 NR 0 TC 8 Z9 8 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56085-5 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 366 BP 165 EP 172 PG 8 WC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics SC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics GA BT23Z UT WOS:A1991BT23Z00019 PM 2068136 ER PT B AU ELBADRY, OM AF ELBADRY, OM BE EVANS, AE DANGIO, GJ KNUDSON, AG SEEGER, RC TI INSULIN-LIKE GROWTH FACTOR-II GENE-EXPRESSION IN HUMAN NEUROBLASTOMA SO ADVANCES IN NEUROBLASTOMA RESEARCH 3 SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT 5TH SYMP ON ADVANCES IN NEUROBLASTOMA RESEARCH CY MAY 28-30, 1990 CL CHILDRENS HOSP, PHILADELPHIA, PA HO CHILDRENS HOSP RP ELBADRY, OM (reprint author), NCI,SURG BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 4 Z9 4 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56085-5 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 366 BP 249 EP 256 PG 8 WC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics SC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics GA BT23Z UT WOS:A1991BT23Z00028 PM 2068141 ER PT B AU ELBADRY, OM MEYERS, MB SPENGLER, BA CHANG, TD ROSS, RA BIEDLER, JL AF ELBADRY, OM MEYERS, MB SPENGLER, BA CHANG, TD ROSS, RA BIEDLER, JL BE EVANS, AE DANGIO, GJ KNUDSON, AG SEEGER, RC TI MEDIUM CONDITIONED BY HUMAN NEUROBLASTOMA BE(2)-C CELLS CONTAINS AN AUTOCRINE/PARACRINE ACTING GROWTH-FACTOR WITH PROPERTIES SIMILAR TO INSULIN-LIKE GROWTH FACTOR-II SO ADVANCES IN NEUROBLASTOMA RESEARCH 3 SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT 5TH SYMP ON ADVANCES IN NEUROBLASTOMA RESEARCH CY MAY 28-30, 1990 CL CHILDRENS HOSP, PHILADELPHIA, PA HO CHILDRENS HOSP RP ELBADRY, OM (reprint author), NCI,SURG BRANCH,BLDG 10,ROOM 2B42,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 0 TC 5 Z9 5 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56085-5 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 366 BP 257 EP 266 PG 10 WC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics SC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics GA BT23Z UT WOS:A1991BT23Z00029 PM 2068142 ER PT B AU COOPER, MJ HUTCHINS, GM COHEN, PS HELMAN, LJ ISRAEL, MA AF COOPER, MJ HUTCHINS, GM COHEN, PS HELMAN, LJ ISRAEL, MA BE EVANS, AE DANGIO, GJ KNUDSON, AG SEEGER, RC TI NEUROBLASTOMA CELL-LINES MIMIC CHROMAFFIN NEUROBLAST MATURATION SO ADVANCES IN NEUROBLASTOMA RESEARCH 3 SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT 5TH SYMP ON ADVANCES IN NEUROBLASTOMA RESEARCH CY MAY 28-30, 1990 CL CHILDRENS HOSP, PHILADELPHIA, PA HO CHILDRENS HOSP RP COOPER, MJ (reprint author), NCI,PEDIAT BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 6 Z9 6 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56085-5 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 366 BP 343 EP 350 PG 8 WC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics SC Biochemistry & Molecular Biology; Oncology; Genetics & Heredity; Pediatrics GA BT23Z UT WOS:A1991BT23Z00038 PM 1676846 ER PT J AU GRACELY, RH AF GRACELY, RH TI EXPERIMENTAL PAIN MODELS SO ADVANCES IN PAIN RESEARCH AND THERAPY LA English DT Review ID NITROUS-OXIDE ANALGESIA; SENSORY DECISION-THEORY; SIGNAL-DETECTION THEORY; EVENT-RELATED POTENTIALS; TOOTH-PULP SENSATIONS; RADIANT-HEAT STIMULI; VISUAL ANALOG SCALES; COLD-INDUCED PAIN; HEALTHY-VOLUNTEERS; PSYCHOPHYSICAL ANALYSIS RP GRACELY, RH (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,CLIN PAIN SECT,BETHESDA,MD 20892, USA. NR 114 TC 12 Z9 12 U1 2 U2 4 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0146-0722 J9 ADV PAIN RES THER JI Adv. Pain Res. Ther. PY 1991 VL 18 BP 33 EP 47 PG 15 WC Neurosciences SC Neurosciences & Neurology GA FD808 UT WOS:A1991FD80800003 ER PT J AU MAX, MB LASKA, EM AF MAX, MB LASKA, EM TI SINGLE-DOSE ANALGESIC COMPARISONS SO ADVANCES IN PAIN RESEARCH AND THERAPY LA English DT Review ID VISUAL ANALOG SCALES; CLINICAL-TRIALS; CANCER-PATIENTS; POSTOPERATIVE PAIN; MORPHINE ANALGESIA; CROSSOVER DESIGNS; BIOASSAY; PLACEBO; PENTAZOCINE; CLONIDINE C1 NATHAN S KLINE INST PSYCHIAT RES,DIV STAT SCI & EPIDEMIOL,ORANGEBURG,NY. NYU MED CTR,DEPT PSYCHIAT,NEW YORK,NY 10016. RP MAX, MB (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,CLIN TRIALS UNIT,BETHESDA,MD 20892, USA. NR 116 TC 55 Z9 55 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0146-0722 J9 ADV PAIN RES THER JI Adv. Pain Res. Ther. PY 1991 VL 18 BP 55 EP 95 PG 41 WC Neurosciences SC Neurosciences & Neurology GA FD808 UT WOS:A1991FD80800005 ER PT J AU MAX, MB AF MAX, MB TI NEUROPATHIC PAIN SYNDROMES SO ADVANCES IN PAIN RESEARCH AND THERAPY LA English DT Review ID POST-HERPETIC NEURALGIA; DOUBLE-BLIND CROSSOVER; DIABETIC NEUROPATHY; POSTHERPETIC NEURALGIA; CLINICAL-TRIAL; NERVE INJURY; AMITRIPTYLINE; PLACEBO; QUESTIONNAIRE; DESIGNS RP MAX, MB (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BETHESDA,MD 20892, USA. NR 81 TC 29 Z9 29 U1 1 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0146-0722 J9 ADV PAIN RES THER JI Adv. Pain Res. Ther. PY 1991 VL 18 BP 193 EP 219 PG 27 WC Neurosciences SC Neurosciences & Neurology GA FD808 UT WOS:A1991FD80800016 ER PT J AU HARGREAVES, KM DIONNE, RA AF HARGREAVES, KM DIONNE, RA TI EVALUATING ENDOGENOUS MEDIATORS OF PAIN AND ANALGESIA IN CLINICAL-STUDIES SO ADVANCES IN PAIN RESEARCH AND THERAPY LA English DT Review ID SUBSTANCE-P RELEASE; BETA-ENDORPHIN IMMUNOREACTIVITY; PLASMA-CATECHOLAMINE RESPONSES; RHEUMATOID-ARTHRITIS; ORAL-SURGERY; ULCERATIVE-COLITIS; ARACHIDONIC-ACID; SURGICAL STRESS; FACTOR CRF; INFLAMMATION C1 NIDR,NEUROBIOL & ANESTHESIOL BRANCH,CLIN PHARMACOL UNIT,BETHESDA,MD 20892. RP HARGREAVES, KM (reprint author), UNIV MINNESOTA,SCH DENT,DEPT RESTORAT SCI,MINNEAPOLIS,MN 55455, USA. NR 98 TC 6 Z9 6 U1 1 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0146-0722 J9 ADV PAIN RES THER JI Adv. Pain Res. Ther. PY 1991 VL 18 BP 579 EP 598 PG 20 WC Neurosciences SC Neurosciences & Neurology GA FD808 UT WOS:A1991FD80800041 ER PT J AU ELING, TE GLASGOW, WC AF ELING, TE GLASGOW, WC TI TRANSDUCTION OF THE EPIDERMAL GROWTH-FACTOR MITOGENIC SIGNAL IN BALB/C 3T3 FIBROBLASTS INVOLVES LINOLEIC-ACID METABOLISM SO ADVANCES IN PROSTAGLANDIN THROMBOXANE AND LEUKOTRIENE RESEARCH LA English DT Review ID ARACHIDONIC-ACID; CELLS; STIMULATION; RELEASE RP ELING, TE (reprint author), NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709, USA. NR 7 TC 2 Z9 2 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0361-5952 J9 ADV PROSTAG THROMB L JI Adv. Prostag. Thrombox. Leukot. Res. PY 1991 VL 21 BP 843 EP 846 PN A-B PG 4 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA EL206 UT WOS:A1991EL20600183 PM 1825423 ER PT J AU KINCAID, RL AF KINCAID, RL TI SIGNALING MECHANISMS IN MICROORGANISMS - COMMON THEMES IN THE EVOLUTION OF SIGNAL TRANSDUCTION PATHWAYS SO ADVANCES IN SECOND MESSENGER AND PHOSPHOPROTEIN RESEARCH LA English DT Review ID DEPENDENT PROTEIN-KINASE; HEAVY-CHAIN GENE; DICTYOSTELIUM-DISCOIDEUM; ADENYLATE-CYCLASE; CYCLIC-AMP; SACCHAROMYCES-CEREVISIAE; PHYSARUM-POLYCEPHALUM; ESCHERICHIA-COLI; BACTERIAL CHEMOTAXIS; GLUTAMINE-SYNTHETASE RP NIAAA, PHYSIOL & PHARMACOL STUDIES LAB, IMMUNOL SECT, ROCKVILLE, MD 20852 USA. NR 77 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1040-7952 J9 ADV SEC MESS PHOSPH JI Adv.Second Messenger Phosphoprotein Res. PY 1991 VL 23 BP 165 EP 184 PG 20 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ER409 UT WOS:A1991ER40900003 ER PT J AU DONAHUE, RE AF DONAHUE, RE TI COLONY-STIMULATING FACTORS - THEIR BIOLOGICAL-ACTIVITIES AND CLINICAL PROMISE SO ADVANCES IN VETERINARY SCIENCE AND COMPARATIVE MEDICINE LA English DT Review ID HUMAN GM-CSF; BONE-MARROW TRANSPLANTATION; CELL GROWTH-FACTOR; RECOMBINANT HUMAN INTERLEUKIN-3; ACQUIRED IMMUNODEFICIENCY SYNDROME; CANINE CYCLIC HEMATOPOIESIS; ACUTE MYELOBLASTIC-LEUKEMIA; MOLECULAR-CLONING; CYTO-TOXICITY; MYELODYSPLASTIC SYNDROMES RP DONAHUE, RE (reprint author), NHLBI,CLIN HEMATOL BRANCH,BETHESDA,MD 20892, USA. NR 152 TC 0 Z9 0 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0065-3519 J9 ADV VET SCI COMP MED JI Adv. Vet. Sci. Comp. Med. PY 1991 VL 36 BP 291 EP 315 PG 25 WC Veterinary Sciences SC Veterinary Sciences GA GV011 UT WOS:A1991GV01100011 PM 1759627 ER PT J AU VIRKKUNEN, M LINNOILA, M AF VIRKKUNEN, M LINNOILA, M TI HOW TO PREDICT IMPULSIVE VIOLENCE - THE BIOCHEMICAL FINDINGS AMONG HABITUALLY VIOLENT OFFENDERS SO AGGRESSIVE BEHAVIOR LA English DT Meeting Abstract C1 UNIV HELSINKI,CENT HOSP,SF-00100 HELSINKI 10,FINLAND. NIAAA,DICBR,CLIN STUDIES LAB,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0096-140X J9 AGGRESSIVE BEHAV JI Aggressive Behav. PY 1991 VL 17 IS 2 BP 101 EP 101 PG 1 WC Behavioral Sciences; Psychology, Multidisciplinary SC Behavioral Sciences; Psychology GA FA659 UT WOS:A1991FA65900058 ER PT J AU HUNTER, L AF HUNTER, L TI ARTIFICIAL-INTELLIGENCE AND MOLECULAR-BIOLOGY SO AI MAGAZINE LA English DT Article AB Molecular biology is emerging as an important domain for artificial intelligence research. The advantages of biology for design and testing of AI systems include large amounts of available online data, significant (but incomplete) background knowledge, a wide variety of problems commensurate with AI technologies, clear standards of success, cooperative domain experts, non-military basic research support and perceived potential for practical (and profitable) applications. These considerations have motivated a growing group of researchers to pursue both basic and applied AI work in the domain. More than seventy-five researchers working on these problems gathered at Stanford for a AAAI sponsored symposium on the topic. This article provides a description of much of the work presented at the meeting, and fills in the basic biology background necessary to place it in context. RP HUNTER, L (reprint author), NATL LIB MED,BETHESDA,MD 20209, USA. OI Hunter, Lawrence/0000-0003-1455-3370 NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER ASSOC ARTIFICIAL INTELL PI MENLO PK PA 445 BURGESS DRIVE, MENLO PK, CA 94025-3496 SN 0738-4602 J9 AI MAG JI AI Mag. PD JAN PY 1991 VL 11 IS 5 SI SI BP 27 EP 36 PG 10 WC Computer Science, Artificial Intelligence SC Computer Science GA EV296 UT WOS:A1991EV29600002 ER PT J AU KAMENGA, M RYDER, RW JINGU, M MBUYI, N MBU, L BEHETS, F BROWN, C HEYWARD, WL AF KAMENGA, M RYDER, RW JINGU, M MBUYI, N MBU, L BEHETS, F BROWN, C HEYWARD, WL TI EVIDENCE OF MARKED SEXUAL-BEHAVIOR CHANGE ASSOCIATED WITH LOW HIV-1 SEROCONVERSION IN 149 MARRIED-COUPLES WITH DISCORDANT HIV-1 SEROSTATUS - EXPERIENCE AT AN HIV COUNSELING-CENTER IN ZAIRE SO AIDS LA English DT Article DE ZAIRE; HIV-1; COUNSELING; SEROLOGY ID HUMAN IMMUNODEFICIENCY VIRUS; AFRICA; TRANSMISSION; EPIDEMIOLOGY; INFECTION AB To determine the effect of an HIV-1 counselling program on 149 married Zairian couples with discordant HIV-1 serology, the rates of HIV-1 seroconversion and reported condom utilization have been observed during 382.4 person-years of follow-up (minimum follow-up time per couple of 6 months). Before determination of HIV-1 serostatus and counselling, less than 5% of these couples had ever used a condom. One month after notification of HIV-1 serostatus and counselling, 70.7% of couples reported using condoms during all episodes of sexual intercourse. At 18 months follow-up, 77.4% of the 140 couples still being followed reported continued use of condoms during all episodes of sexual intercourse. At the time of notification of HIV-1 serostatus, 18 couples experienced acute psychological distress. Home-based counselling by trained nurses resolved these difficulties in all but three couples who subsequently divorced. Intensive counselling following notification of HIV-1 serostatus led to low rates of HIV-1 seroconversion (3.1% per 100 person-years of observation) in Zairian married couples with discordant HIV-1 serostatus who voluntarily attended an HIV counselling center. C1 CTR DIS CONTROL,CTR INFECT DIS,DIV HIV AIDS,ATLANTA,GA 30333. NIAID,BETHESDA,MD 20892. RP KAMENGA, M (reprint author), DEPT PUBL HLTH,PROJET SIDA,KINSHASA,ZAIRE. NR 13 TC 196 Z9 197 U1 2 U2 5 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0269-9370 J9 AIDS JI Aids PD JAN PY 1991 VL 5 IS 1 BP 61 EP 67 PG 7 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA FA037 UT WOS:A1991FA03700009 PM 2059362 ER PT J AU BROWN, C KLINE, R ATIBU, L FRANCIS, H RYDER, R QUINN, TC AF BROWN, C KLINE, R ATIBU, L FRANCIS, H RYDER, R QUINN, TC TI PREVALENCE OF HIV-1 P24 ANTIGENEMIA IN AFRICAN AND NORTH-AMERICAN POPULATIONS AND CORRELATION WITH CLINICAL STATUS SO AIDS LA English DT Note DE HIV-1 INFECTION; P24 ANTIGEN; AIDS; AFRICAN POPULATIONS; NORTH AMERICAN POPULATIONS ID IMMUNODEFICIENCY VIRUS-INFECTION; AIDS; ANTIBODIES; SERUM AB Sera from 622 individuals and culture supernatants from three HIV-1 viral isolates were assayed for HIV-1 p24 antigen to investigate the frequency of p24 antigenemia in African and North American populations using three commercial HIV-1 p24 antigen assays (Coulter, Du Pont, and Abbott). The prevalence of p24 antigenemia in 89 hospitalized Zairian AIDS patients was significantly lower than in 47 clinically comparable AIDS patients in the USA (17 versus 48%, P < 0.0001). Prevalence of p24 antigenemia in sera from 200 asymptomatic HIV-1-infected individuals was also lower in individuals from Zaire compared with 83 individuals in the USA (3.5 versus 7%). In African individuals, antigenemia prevalence increased with advanced clinical status: 8% in ambulatory AIDS patients, 17% in hospitalized AIDS patients and 18% in postmortem AIDS patients. Acid hydrolysis treatment of sera from 63 Zairian AIDS patients initially negative for p24 antigen showed an 11% positivity rate confirmed by neutralization, suggesting that immune complexing of p24 antigen may play a role in the observed lower p24 antigenemia rates reported for African individuals. C1 JOHNS HOPKINS UNIV,DIV INFECT DIS,BLALOCK 1111,600 N WOLFE ST,BALTIMORE,MD 21205. PROJET SIDA,KINSHASA,ZAIRE. CTR DIS CONTROL,ATLANTA,GA 30333. NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NR 14 TC 28 Z9 28 U1 0 U2 0 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0269-9370 J9 AIDS JI Aids PD JAN PY 1991 VL 5 IS 1 BP 89 EP 92 PG 4 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA FA037 UT WOS:A1991FA03700013 PM 1905553 ER PT J AU GRUBER, MF WEBB, DSA GERRARD, TL MOSTOWSKI, HS VUJCIC, L GOLDING, H AF GRUBER, MF WEBB, DSA GERRARD, TL MOSTOWSKI, HS VUJCIC, L GOLDING, H TI REEVALUATION OF THE INVOLVEMENT OF THE ADHESION MOLECULES ICAM-1/LFA-1 IN SYNCYTIA FORMATION OF HIV-1-INFECTED SUBCLONES OF A CEM T-CELL LEUKEMIC LINE SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; TUMOR NECROSIS FACTOR; ENDOTHELIAL-CELLS; AIDS RETROVIRUS; ANTIGEN-1 LFA-1; INTERFERON; INDUCTION; INFECTION; RECEPTOR AB The role of the adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and LFA-1 in human immunodeficiency virus type 1 (HIV-1)-induced cell fusion was investigated in subclones of a T-cell leukemic cell line (CEM) with differing abilities to form synctia. Addition of monoclonal antibodies 84H10 directed against ICAM-1 and MHM23 directed against the common-beta-subunit of LFA-1 (CD18) resulted in greater than 50% suppression of syncytia formation in cultures of these clones infected with cell-free virus. Two subclones, 2G5-144-84 and 2G5-1, were deficient in their ability to form syncytia and expressed reduced amounts of LFA-1 compared with the parental line. The expression of ICAM-1 but not LFA-1 was upregulated on the clones following treatment with interferon-gamma (IFN-gamma); however, this did not overcome the delay in syncytia formation observed in these cells. The syncytia-positive subclones 1B11-39 and 17D-9 expressed high levels of LFA-1. Basal expression of ICAM-1 was upregulated on these cells by treatment with tumor necrosis factor-alpha (TNF-alpha), which also accelerated and enhanced syncytia formation. However, anti-ICAM-1 and anti-LFA-1 (CD18) antibodies did not reverse the TNF-alpha-induced enhancement of syncytia formation of HIV-1-infected clones 1B11-39 and 17D-9. Under conditions of low viral expression, adhesion molecules may contribute to syncytia formation if adequate levels of both receptor and ligand in the ICAM-1/LFA-1 complex are expressed. However, our data do not support the concept of an absolute requirement for those molecules in HIV-1-induced cell fusion, particularly under conditions in which large amounts of gp160 are expressed on the surface of the virus-infected cell. C1 NIH,US FDA,CTR BIOL EVALUAT & RES,DIV VIROL,BETHESDA,MD 20892. RP GRUBER, MF (reprint author), NIH,US FDA,CTR BIOL EVALUAT & RES,DIV CYTOKINE BIOL,BLDG 29A,BETHESDA,MD 20892, USA. NR 17 TC 47 Z9 49 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD JAN PY 1991 VL 7 IS 1 BP 45 EP 53 PG 9 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA EY676 UT WOS:A1991EY67600006 PM 1707641 ER PT J AU PRIEL, E SHOWALTER, SD BLAIR, DG AF PRIEL, E SHOWALTER, SD BLAIR, DG TI INHIBITION OF HUMAN-IMMUNODEFICIENCY-VIRUS (HIV-1) REPLICATION INVITRO BY NONCYTOTOXIC DOSES OF CAMPTOTHECIN, A TOPOISOMERASE-I INHIBITOR SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID SITE-SPECIFIC RECOMBINATION; DNA; CELLS; INFECTIVITY; MECHANISM; CLEAVAGE; PROTEINS; MUTANTS AB We examined the effects of topoisomerase inhibitors on human immunodeficiency virus type 1 (HIV-1) infection of H9 cells in cell culture. Infection is blocked or substantially reduced by the topoisomerase I inhibitor camptothecin (CPT), but not by two topoisomerase II inhibitors. Significantly reduction (greater-than-or-equal-to 90%) in the amount of virus released, as measured by reverse transcriptase, is obtained if the cells are treated for 1 h with 0.01-0.02-mu-M CPT at the time of virus infection, and expression of viral proteins is also blocked. CPT is also shown to reduce the level of infection when chronically infected cells are cocultivated with unifected cells. These results with CPT suggest that this compound may represent a new class of drugs with antiretroviral potential. C1 PROGRAM RESOURCES INC,NUCLEIC ACID & PROT SYNTH LAB,FREDERICK,MD 21702. NCI,MOLEC ONCOL LAB,FREDERICK,MD 21702. RP PRIEL, E (reprint author), BEN GURION UNIV NEGEV,FAC HLTH SCI,DEPT MICROBIOL & IMMUNOL,IL-84105 BEER SHEVA,ISRAEL. NR 26 TC 85 Z9 95 U1 0 U2 1 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD JAN PY 1991 VL 7 IS 1 BP 65 EP 72 PG 8 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA EY676 UT WOS:A1991EY67600008 PM 1707642 ER PT J AU RECKER, DP KULAGA, H DORSETT, D FOLKS, T KINDT, TJ AF RECKER, DP KULAGA, H DORSETT, D FOLKS, T KINDT, TJ TI A MONOCYTE-DERIVED FACTOR INTERFERES WITH DETECTION OF REVERSE-TRANSCRIPTASE IN HIV-1 INFECTION SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; DEPENDENT DNA POLYMERASE; HTLV-III; T-CELL; RETROVIRUS; AIDS; LAV; RECEPTOR; VIRIONS; LINES AB Culture supernatants from the rabbit macrophage cell line 6083 infected with a retrovirus, human immunodeficiency virus type 1 (HIV-1), were negative for reverse transcriptase (RT) expression although the line was shown to be productively infected by all other criteria tested. Supernatants from uninfected cultures of 6083, the human monocyte line U937, and from freshly isolated peripheral human monocytes, were found to contain a monocyte-derived inhibitory factor (MDIF) which interferes with a standard assay for RT. MDIF is a heat-labile activity of approximately of 40 kD. Both substrates and products of the reverse transcriptase assay are degraded by MDIF which is not affected by reduction and alkylation of disulfide bonds. MDIF is inhibited by the addition of a particular thioated oligonucleotide (S-dG30) to the reaction mixture but this addition also inhibits RT. The optimum method to minimize MDIF interference in the RT assay is by addition of ethylene glycol bis-(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA); MDIF requires divalent cations for activity and has a strong preference for calcium which is preferentially chelated by EGTA. The potential presence of this inhibitory activity should be conisdered when using RT levels as a measure of retroviral infection. C1 NIAID,IMMUNOGENET LAB,TWINBROOK FACIL,12441 PARKLAWN DR,BETHESDA,MD 20892. NIMH,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. EMORY UNIV,SCH MED,DEPT PATHOL,ATLANTA,GA 30322. CTR DIS CONTROL,RETROVIRUS DIS BRANCH,ATLANTA,GA 30333. NR 23 TC 8 Z9 8 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD JAN PY 1991 VL 7 IS 1 BP 73 EP 81 PG 9 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA EY676 UT WOS:A1991EY67600009 PM 1707643 ER PT J AU ULRICHSEN, J AF ULRICHSEN, J TI ALTERATIONS IN SEROTONIN RECEPTOR SUBTYPES IN ETHANOL-DEPENDENT RATS SO ALCOHOL AND ALCOHOLISM LA English DT Article ID BINDING; BRAIN; 5-HYDROXYTRYPTAMINE; WITHDRAWAL; TOLERANCE; REGIONS; 5-HT1B; SITES AB Serotonin (5-HT) receptor subtypes were investigated during severe ethanol intoxication and withdrawal. Ethanol was administered intragastrically five times a day for 4 days (12 g/kg per day). 5-HT receptor subtypes were studied: (1) in severely intoxicated animals (mean blood ethanol concentration (BEC) = 4.7 g/l); (2) during the withdrawal reaction; and (3) in a control group. The maximal density of [H-3] 8-hydroxy-2-(di-n-propylamino)tetralin ([H-3] 8-OH-DPAT) binding (B(max)) to 5-HT1a receptors was decreased by 25 and 17% in the hippocampus during chronic ethanol intoxication and withdrawal, respectively. [H-3]Ketanserin binding to 5-HT2 receptors in the cortex, (-) [I-125]-iodo-cyanopindolol ([I-125]CYP) binding to 5-HT1b receptors in the striatum and hypothalamus, and [H-3] 8-OH-DPAT binding in the cortex were not affected by chronic ethanol administration. Previous in vitro experiments have shown that 5-HT1a receptors in the hippocampus are inhibitory. The down-regulation of these receptors may play a role in physical ethanol dependence, by inducing hyperexcitability of the hippocampus. RP ULRICHSEN, J (reprint author), NIDA,ADDICT RES CTR,CLIN PSYCHOPHARMACOL LAB,POB 5180,BALTIMORE,MD 21224, USA. NR 30 TC 23 Z9 23 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 VL 26 IS 5-6 BP 567 EP 573 PG 7 WC Substance Abuse SC Substance Abuse GA HE065 UT WOS:A1991HE06500011 PM 1839497 ER PT J AU TABAKOFF, B HOFFMAN, PL AF TABAKOFF, B HOFFMAN, PL TI RECENT ADVANCES IN ALCOHOL RESEARCH - 1990 (ISBRA PRESIDENTIAL-ADDRESS) SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE BRAIN; GABA; SEROTONIN; NMDA; VASOPRESSIN; TOLERANCE; DEPENDENCE ID D-ASPARTATE RECEPTORS; HIPPOCAMPAL SLICE; ETHANOL; RAT; NEURONS; IMIDAZOBENZODIAZEPINE; INHIBITION; INCREASES; TOLERANCE; RELEASE C1 NIAAA, DIV INTRAMURAL CLIN & BIOL RES, ROCKVILLE, MD 20852 USA. RP TABAKOFF, B (reprint author), UNIV COLORADO, HLTH SCI CTR, DEPT PHARMACOL, DENVER, CO 80262 USA. NR 31 TC 4 Z9 4 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 1 EP 7 PG 7 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500002 ER PT J AU ALKON, DL MATZEL, LD COLLIN, C AF ALKON, DL MATZEL, LD COLLIN, C TI MOLECULAR MECHANISMS OF MEMORY AND DRUG-DEPENDENCE SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE ASSOCIATIVE MEMORY; ADDICTION; G-PROTEIN; PROTEIN KINASE-C; K+ CURRENTS; PHOSPHORYLATION ID PROTEIN AB Addiction has long been thought to include both metabolic and psychological dependence. Psychological dependence must involve long-term memory of behavioral patterns in response to specific experiential contexts. Mammalian memory, and more specifically, human memory, is largely associative. Animal models of associative memory have been provided by Pavlovian conditioning of the snail Hermissenda crassicornis and the rabbit. Striking parallels have been observed in the intrinsic molecular and biophysical transformations which accompany acquisition of the conditioned response in these different animals. In brief, associated stimuli cause elevation of Ca2+ and diacylglycerol, translocation of protein kinase C, phosphorylation of a membrane-associated G-protein, reduction of K+ currents, modification of axonal transport and structural alterations of neuronal branches. These changes can be understood and modelled as a plausible basis for memory acquisition during conditioning as well as more cognitively relevant learning such as spatial maze learning for which related neuronal alterations have recently been found. Identification of memory-specific molecular steps may help target pharmacologic agents for amelioration of learned aspects of psychiatric syndromes such as those of drug dependence. RP ALKON, DL (reprint author), NIH,NEURAL SYST SECT,MOLEC & CELLULAR NEUROBIOL LAB,BETHESDA,MD 20892, USA. NR 5 TC 1 Z9 1 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 35 EP 37 PG 3 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500006 ER PT J AU GORDIS, E AF GORDIS, E TI SCIENCE AND SOCIAL-POLICY PROBLEMS - RESOLUTION OF SOME, CREATION OF OTHERS SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE ALCOHOLISM; PUBLIC POLICY; GENETIC SCREENING; RESEARCH AGENDAS; MEDICAL PRACTICE; DECISION MAKING; ALCOHOL AND DRUG ABUSE; LEGISLATION ID LIVER-TRANSPLANTATION AB The scientist has two jobs: to do good science, and to be a spokesperson for science in the creation of rational social policy. Three categories of interaction between science and policy are described and examples given. The first is where science has provided data that were decisive in causing policy to be enacted. The second is where science is capable of resolving equity issues that have arisen from problems that science itself created. The third is where the issues are ultimately resolved by extrascientific considerations. Two major issues where it is hoped that science can make a significant contribution include outcomes analysis, a new approach to dealing with health care costs and variations in clinical practice; and the disease concept of alcoholism, with questions it raises of volition, personal responsibility, and punishment. However, science alone is never the dominant force in the shaping of policy, which is determined by the interaction of many social forces, such as morals, values, politics, and economics. RP GORDIS, E (reprint author), NIAAA,ROCKVILLE,MD 20852, USA. NR 20 TC 0 Z9 0 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 65 EP 76 PG 12 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500010 ER PT J AU GONZALEZ, FJ UENO, T UMENO, M SONG, BJ VEECH, RL GELBOIN, HV AF GONZALEZ, FJ UENO, T UMENO, M SONG, BJ VEECH, RL GELBOIN, HV TI MICROSOMAL ETHANOL OXIDIZING SYSTEM - TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL REGULATION OF CYTOCHROME-P450, CYP2E1 SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE CYTOCHROME-P450; CYP2E1; HNF-1; PROTEIN STABILIZATION; MESSENGER RNA STABILIZATION ID LIVER-MICROSOMES; INDUCIBLE CYTOCHROME-P-450; TREATED RABBITS; GROWTH-HORMONE; DIABETIC RAT; METABOLISM; ACETONE; PURIFICATION; EXPRESSION; P450IIE1 AB CYP2E1 is solely responsible for microsomal P450-mediated ethanol oxidation activity. This enzyme is also involved in a pathway leading to gluconeogenesis from ketone bodies and in metabolic activation of numerous foreign compounds to intermediates that can be toxic to cells. Metabolic activation of certain procarcinogens by CYP2E1 may also lead to cell transformation. Regulation of CYP2E1 is especially intriguing. The CYP2E1 gene is transcriptionally activated in rat liver from a dormant state within a few hours after birth. This activation is due in part to the participation of a transcription factor designated hepatocyte nuclear factor 1 or HNF-1. In adult animals, constitutive expression of the enzyme is controlled to some degree by growth hormone. CYP2E1 is also regulated by many of its substrates through a substrate-induced stabilization of the enzyme. Under extreme conditions of fasting and uncontrolled diabetes CYP2E1 mRNA is stabilized. C1 NIAAA,METAB & MOLEC BIOL LAB,BETHESDA,MD 20892. RP GONZALEZ, FJ (reprint author), NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. NR 39 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 97 EP 101 PG 5 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500014 ER PT J AU HABER, R GOLDMAN, D AF HABER, R GOLDMAN, D TI EXPRESSION OF MOUSE SEROTONIN TRANSPORTER RNA IN XENOPUS-LAEVIS OOCYTES SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE SEROTONIN TRANSPORTER; BRAIN-STEM; RNA; ANTIDEPRESSANT DRUGS ID PLASMA-MEMBRANE VESICLES; BLOOD-PLATELETS; CEREBRAL-CORTEX; BINDING-SITES; RAT-BRAIN; IMIPRAMINE; 5-HYDROXYTRYPTAMINE; IMIPRAMINE; SYNAPTOSOMES AB The serotonin transporter removes serotonin from the synapse, terminating the action of this neurotransmitter. We have taken an initial step towards cloning the serotonin transporter by obtaining its expression in Xenopus oocytes. We found that mouse brainstem is a suitable source of serotonin transporter mRNA and that synthesis of the transporter in injected oocytes occurs steadily over a 3 day period. Uptake of [H-3] serotonin was inhibited by 18 nM citalopram, a specific inhibitor of serotonin transport. RP HABER, R (reprint author), NIAAA,CLIN STUDIES LAB,ROCKVILLE,MD 20852, USA. RI Goldman, David/F-9772-2010 OI Goldman, David/0000-0002-1724-5405 NR 38 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 125 EP 130 PG 6 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500019 ER PT J AU DEVOR, EJ CLONINGER, CR HOFFMAN, PL TABAKOFF, B AF DEVOR, EJ CLONINGER, CR HOFFMAN, PL TABAKOFF, B TI ADENYLATE-CYCLASE ACTIVITY IN THE FAMILIES OF ALCOHOLICS IS CONTROLLED BY A SINGLE MAJOR GENE SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE ADENYLATE CYCLASE; ALCOHOLISM; FAMILY STUDY; GENETICS; SEGREGATION ANALYSIS; MAJOR GENE ID COMPLEX SEGREGATION ANALYSIS; HETEROGENEITY AB The stimulated activity of platelet adenylate cyclase (AC) has been shown to be significantly lower among alcoholics compared with controls. In this report we demonstrate that stimulated platelet AC activity is under the control of a single major gene locus. Thus a sensitive biological indicator of alcoholism has been shown to be highly heritable. C1 NIAAA,BETHESDA,MD 20892. RP DEVOR, EJ (reprint author), WASHINGTON UNIV,SCH MED,DEPT PSYCHIAT,ST LOUIS,MO 63110, USA. RI Cloninger, Claude/F-5357-2012 OI Cloninger, Claude/0000-0003-3096-4807 NR 12 TC 2 Z9 2 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 157 EP 160 PG 4 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500025 ER PT J AU WEIGHT, FF LOVINGER, DM WHITE, G AF WEIGHT, FF LOVINGER, DM WHITE, G TI ALCOHOL INHIBITION OF NMDA CHANNEL FUNCTION SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE ETHANOL; NMDA CHANNEL; ION CURRENTS; NEURONS; INTOXICATION ID GAMMA-AMINOBUTYRIC ACID; LONG-TERM POTENTIATION; D-ASPARTATE RECEPTORS; HIPPOCAMPAL SLICE; PYRAMIDAL CELLS; RAT-BRAIN; ETHANOL; NEURONS; ACETYLCHOLINE; RESPONSES AB In mammalian central neurons, intoxicating concentrations of ethanol inhibit the ion current activated by the glutamate agonist N-methyl-D-aspartate (NMDA). Electrophysiologic analysis of the molecular mechanism involved in this inhibition indicates that ethanol does not inhibit NMDA-activated ion current by voltage-dependent block of the channel, altering the ion selectivity of the channel, or altering the affinity of binding sites for NMDA, glycine or substances known to regulate the function of this channel (Mg2+, Zn2+ and ketamine). The potency for inhibiting the NMDA-activated current by different alcohols is linearly related to their hydrophobicity, suggesting that alcohols may inhibit the NMDA-activated current by a novel type of interaction with a hydrophobic region of the channel. RP WEIGHT, FF (reprint author), NIAAA,ELECTROPHYSIOL SECT,ROCKVILLE,MD 20852, USA. NR 34 TC 3 Z9 3 U1 2 U2 3 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 163 EP 169 PG 7 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500026 ER PT J AU HOFFMAN, PL TABAKOFF, B AF HOFFMAN, PL TABAKOFF, B TI THE CONTRIBUTION OF VOLTAGE-GATED AND NMDA RECEPTOR-GATED CALCIUM CHANNELS TO ETHANOL WITHDRAWAL SEIZURES SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE ETHANOL WITHDRAWAL SEIZURES; VOLTAGE-DEPENDENT CALCIUM CHANNELS; NMDA RECEPTOR-GATED CALCIUM CHANNELS; ETHANOL PHYSICAL DEPENDENCE ID METHYL-D-ASPARTATE; ALCOHOL-WITHDRAWAL; HIPPOCAMPAL-NEURONS; PYRAMIDAL CELLS; MOUSE LINES; RAT; ANTAGONISTS; ACID; MODULATION; INHIBITION RP HOFFMAN, PL (reprint author), NIAAA, DIV INTRAMURAL CLIN & BIOL RES, ROCKVILLE, MD 20852 USA. NR 41 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 171 EP 175 PG 5 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500027 ER PT J AU LOVINGER, DM AF LOVINGER, DM TI ETHANOL POTENTIATES ION CURRENT MEDIATED BY 5-HT3 RECEPTORS ON NEUROBLASTOMA-CELLS AND ISOLATED NEURONS SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE ALCOHOL; SEROTONIN; NODOSE GANGLION; ION CHANNELS; INTOXICATION; PATCH-CLAMP ID RAT-BRAIN; INHIBITION; CHANNELS; RELEASE; LINES AB Effects of ethanol on receptor/channel complexes appear to play an important role in acute intoxication. One such receptor that has not previously been investigated for ethanol sensitivity is the 5-HT3 receptor for the neurotransmitter serotonin. Ethanol potentiates ion current mediated by 5-HT3 receptors in NCB-20 neuroblastoma cells and isolated Nodose ganglion neurons examined with whole-cell patch-clamp recording. Potentiation increases in a concentration-dependent manner over a range of concentrations (25-100 mM) achieved during acute intoxication. Potentiation appears to be due to a direct effect on the 5-HT3 receptor. Ethanol's effect on 5-HT3 receptor-mediated current decreases with increasing agonist concentration, providing an initial clue as to the mechanism of ethanol's action. These data are discussed in light of recent behavioral data suggesting a role for 5-HT3 receptors in the discriminative stimulus and reinforcing properties of ethanol. RP LOVINGER, DM (reprint author), NIAAA,LPPS,SYNAPT PHARMACOL UNIT,ROCKVILLE,MD 20852, USA. NR 20 TC 3 Z9 3 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 181 EP 185 PG 5 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500029 ER PT J AU AGUAYO, LG AF AGUAYO, LG TI DEMONSTRATION THAT ETHANOL POTENTIATES THE GABA-A-ACTIVATED CL- CURRENT IN CENTRAL MAMMALIAN NEURONS SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE GABA-A RECEPTORS; CL- CURRENT; BARBITURATES; BENZODIAZEPINES; ETHANOL; HIPPOCAMPUS ID POSTSYNAPTIC CURRENTS; CHLORIDE CHANNELS; PYRAMIDAL CELLS; RECEPTORS AB Previous studies have suggested that ethanol potentiates GABA-mediated responses in the mammalian brain. In the present study, using patch-clamp techniques, we show that the GABA(A)-activated Cl- current was potentiated by ethanol in a subpopulation of mouse hippocampal cells. RP AGUAYO, LG (reprint author), NIAAA,ELECTROPHYSIOL SECT,PHYSIOL & PHARMACOL STUDIES LAB,ROCKVILLE,MD 20852, USA. NR 14 TC 0 Z9 1 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 187 EP 190 PG 4 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500030 ER PT J AU CLARK, M DAR, MS AF CLARK, M DAR, MS TI INVITRO AUTORADIOGRAPHIC EVIDENCE FOR ADENOSINE MODULATION OF ETHANOL-INDUCED MOTOR DISTURBANCES IN RATS SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE ADENOSINE A1 AND A2A RECEPTORS; ETHANOL; GUANINE NUCLEOTIDES; INVITRO AUTORADIOGRAPHY; RAT BRAIN ID SELECTIVELY BRED MICE; CEREBELLAR SYNAPTOSOMES; BEHAVIORAL SENSITIVITY; GUANINE-NUCLEOTIDE; CATION REGULATION; CYCLIC-AMP; RECEPTORS; INCOORDINATION; ACCUMULATION; MEMBRANES AB It was previously shown that adenosine agonists and antagonists potentiate and decrease ethanol-induced motor disturbances, respectively. This interaction of adenosine and ethanol was functionally correlated with a significant increase in B(max) of cerebellar cortical high affinity adenosine A1 receptors after a single ethanol injection. Quantitative autoradiographic analysis of adenosine A1 and A2 binding sites in rat brain was carried out in animals treated acutely with saline or ethanol. Adenosine agonist binding at A1 receptors was increased in the molecular layer of cerebellum 15 min after ethanol injection. This increase returned to control values by 60 min. Adenosine antagonist binding at A1 receptors was not altered by ethanol treatment. Inclusion of a poorly hydrolyzable analogue of GTP in the incubation medium decreased binding throughout the brain for adenosine agonists but had less effect on agonist binding in the cerebellum and hippocampus of ethanol-treated rats 15 min after injection. The inhibitory effect of the GTP analogue was equal in saline- and ethanol-treated rats after 60 min. These findings suggest that acute ethanol treatment elicits a transitory increase in adenosine A1 receptor binding that is limited to the cerebellum and hippocampus and that this increased binding reflects an increased (or stabilized) coupling of the receptors to GTP-binding proteins. Acute ethanol treatment did not alter agonist binding at the high affinity A2a subtype of adenosine receptors in striatum. C1 E CAROLINA UNIV,SCH MED,DEPT PHARMACOL,GREENVILLE,NC 27858. RP CLARK, M (reprint author), NIMH,BIOL PSYCHIAT BRANCH,9000 ROCKVILLE PIKE,BLDG 10,ROOM 3N212,BETHESDA,MD 20892, USA. NR 21 TC 1 Z9 1 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 203 EP 206 PG 4 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500033 ER PT J AU PARRISH, KM DUFOUR, MC AF PARRISH, KM DUFOUR, MC TI DRINKING PATTERNS AND LIVER-CIRRHOSIS MORTALITY SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE LIVER CIRRHOSIS; ALCOHOL CONSUMPTION; RISK FACTOR ID ALCOHOL; DISEASE; WOMEN AB Using the 1986 National Mortality Followback Survey, alcohol consumption patterns were compared for decedents with and without mention of cirrhosis of the liver as a cause of death. Approximately 55% of cirrhosis decedents had 3 drinks or more daily (80% of decedents with alcoholic cirrhosis, and 40% of decedents with unspecified or other specified cirrhosis). In contrast, only 10% of decedents without cirrhosis had at least 3 drinks daily. Forty percent of decedents with alcoholic cirrhosis had 7 drinks or more daily, compared with 17% for unspecified cirrhosis, and 21% for other specified cirrhosis. The comparable figure was 3% for decedents without cirrhosis. An average of 3 drinks per day was associated with increased cirrhosis proportional mortality, and cirrhosis proportional mortality increased with higher numbers of daily drinks. C1 NIAAA,ROCKVILLE,MD 20857. RP PARRISH, KM (reprint author), ALCOHOL EPIDEMIOL DATA SYST,1400 EYE ST,WASHINGTON,DC 20005, USA. OI Makimoto, Kiyoko/0000-0003-0242-1290 NR 5 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 331 EP 334 PG 4 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500056 ER PT J AU GEORGE, FR RITZ, MC AF GEORGE, FR RITZ, MC TI COMMON MECHANISMS OF REINFORCEMENT FROM ALCOHOL AND OTHER DRUGS SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE BEHAVIOR GENETICS; OPERANT BEHAVIOR; DRUG SELF-ADMINISTRATION; ALCOHOL; COCAINE; OPIATES ID UPTAKE BLOCKADE; ETHANOL; RATS; SEROTONIN; AMPHETAMINE; COCAINE; RECEPTORS; DOPAMINE; INVOLVEMENT; ATTENUATION AB Vulnerability to substance abuse is an important social and scientific issue. A critical aspect of this phenomenon is the degree to which individuals who abuse one substance are likely to abuse other substances. The extent to which several pharmacologically distinct drugs will come to serve as positive reinforcers within genetically defined subjects defines their commonality with respect to abuse liability. Questions in this area are directed at determining whether reinforcement from and abuse of alcohol and other drugs define variations within a single behavioral phenomenon, or whether reinforcement and abuse must be individually defined for each substance involved. Previous studies have shown that ethanol can be readily established as a positive reinforcer in LEWIS rats and C57BL/6J mice. In low ethanol preferring F344 rats, ethanol maintains significant but low levels of responding. Ethanol does not maintain behavior in BALB/cJ or DBA/2J mice. These genotypic patterns of reinforcement from ethanol appear to correlate highly with patterns of reinforcement from cocaine and opiates. Other studies suggest that some aspects of drug self-administration may be mediated in part by common dopaminergic and serotonergic mechanism, and animals which differ in ethanol drinking behavior show substantial differences in parameters of serotonergic function. Thus, there may exist important genetic determinants of drug reinforced behavior. C1 UNIV MARYLAND,SCH PHARM,DEPT PHARMACOL & TOXICOL,BALTIMORE,MD 21201. RP GEORGE, FR (reprint author), NIDA,ADDICT RES CTR,PRECLIN PHARMACOL BRANCH,BALTIMORE,MD 21224, USA. NR 35 TC 0 Z9 0 U1 1 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 427 EP 431 PG 5 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500073 ER PT J AU FULLER, RK AF FULLER, RK TI ASSESSMENT OF TREATMENT OUTCOME SO ALCOHOL AND ALCOHOLISM LA English DT Article; Proceedings Paper CT 5TH CONGRESS OF THE INTERNATIONAL SOC FOR BIOMEDICAL RESEARCH ON ALCOHOLISM / 1990 ANNUAL MEETING OF THE RESEARCH ON ALCOHOLISM CY JUN 17-22, 1990 CL TORONTO, CANADA SP INT SOC BIOMED RES ALCOHOLISM, RES SOC ALCOHOLISM, ADDICT RES FDN ONTARIO, ALCOHOL BEVERAGES MED RES COUNCIL, ALKO STATE ALCOHOL MONOPOLY, ASTRA PHARMA, BELLWOOD HLTH SERV, BOEHRINGER MANNHEIM CANADA, CHARTER MED, CIBA GEIGY DE ALCOHOLISM TREATMENT RESEARCH; CONTROLLED CLINICAL TRIALS; PROGRAM EVALUATION CONTROL GROUPS; RANDOMIZATION; BLINDING; FOLLOW-UP; ATTRITION; COMPLIANCE; DATA ANALYSIS ID SELF-REPORT; ALCOHOLISM; TRIALS; DESIGN AB Treatment outcome research consists of two types: controlled clinical trials and in-house program evaluations. Controlled clinical trials are experimental studies designed to test whether or not a treatment is efficacious. In-house program evaluations provide information about the results (outcome) of an individual treatment program. The key components of clinical trials include control groups; randomization procedures; enumeration of those who were screened for inclusion in, those accepted into, and those excluded from the study; a description of the relevant patient characteristics of the sample studied; "double-blinding" for pharmacological studies; raters of treatment effects who are not the therapists for verbal therapies; objective measures of treatment response; follow-up of at least 70% of the original sample; and appropriate statistical analysis of the data collected. In-house program evaluations rarely have control groups or employ randomization. However, a description of the type of patients treated by the program, independent raters, and adequate follow-up of those entering treatment are essential for program evaluation; and the credibility of program evaluation would be enhanced by the use of objective measures of treatment response. RP FULLER, RK (reprint author), NIAAA,DIV CLIN & PREVENT RES,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. NR 9 TC 0 Z9 0 U1 2 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PY 1991 SU 1 BP 519 EP 522 PG 4 WC Substance Abuse SC Substance Abuse GA HC925 UT WOS:A1991HC92500091 ER PT J AU CLAMAN, DL RADEBAUGH, TS AF CLAMAN, DL RADEBAUGH, TS TI NEUROPSYCHOLOGICAL ASSESSMENT IN CLINICAL-TRIALS OF ALZHEIMER-DISEASE SO ALZHEIMER DISEASE & ASSOCIATED DISORDERS LA English DT Article; Proceedings Paper CT SATELLITE SYMP OF THE 17TH CONGRESS OF COLLEGIUM-INTERNATIONALE-NEURO-PSYCHOPHARMACOLOGICUM : DIAGNOSTIC AND THERAPEUTIC ASSESSMENT IN DEMENTIA TRIALS CY SEP 15, 1990 CL KYOTO, JAPAN SP COLLOGIUM INT NEUROPSYCHOPHARMACOLOGICUM DE ALZHEIMER DISEASE; DRUG EFFICACY; NEUROPSYCHOLOGICAL ASSESSMENT ID DEMENTIA AB A fundamental problem in research aimed at developing and testing new treatments for Alzheimer disease (AD) is determing drug efficacy. At the present time, while there are no biological markers for diagnosing or assessing this disease, investigators must rely on clinical judgment and neuropsychological outcome measures to determine if and when a new treatment may be beneficial. The following article provides an overview of the issues involved in conducting a clinical trial in AD when measures of cognitive and behavioral changes are used. It is based on the proceedings from an international workshop on this topic that was convened by the National Institute on Aging in January 1990. C1 NIA,BETHESDA,MD 20892. NR 10 TC 2 Z9 2 U1 1 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0893-0341 J9 ALZ DIS ASSOC DIS JI Alzheimer Dis. Assoc. Dis. PY 1991 VL 5 SU 1 BP S49 EP S56 DI 10.1097/00002093-199100051-00009 PG 8 WC Clinical Neurology; Pathology SC Neurosciences & Neurology; Pathology GA GU428 UT WOS:A1991GU42800009 PM 1781974 ER PT J AU KHACHATURIAN, ZS AF KHACHATURIAN, ZS TI OVERVIEW OF BASIC RESEARCH ON ALZHEIMER-DISEASE - IMPLICATIONS FOR COGNITION SO ALZHEIMER DISEASE & ASSOCIATED DISORDERS LA English DT Article; Proceedings Paper CT SATELLITE SYMP OF THE 17TH CONGRESS OF COLLEGIUM-INTERNATIONALE-NEURO-PSYCHOPHARMACOLOGICUM : DIAGNOSTIC AND THERAPEUTIC ASSESSMENT IN DEMENTIA TRIALS CY SEP 15, 1990 CL KYOTO, JAPAN SP COLLOGIUM INT NEUROPSYCHOPHARMACOLOGICUM DE RESEARCH; BRAIN CHEMISTRY; CA2+; CYTOSOL; DENDRITES; CELL DEATH ID NEURONS AB During the past 12 years, research on the etiology of Alzheimer disease (AD) has made dramatic advances. Although we still do not have a clear picture of what factor(s) cause AD, growing evidence indicates that the fundamental problem in AD is associated with the death of neurons. The purpose of this article is to re-evaluate in detail how the regulation of intracellular free calcium might play a critical role in cell death and in the expression of AD neuropathology. There is an urgent need to find means to ameliorate the symptoms of AD and ways to slow the progression of the disease process. Unfortunately, AD appears to be a very complex neurochemical puzzle. C1 NIA,BETHESDA,MD 20892. NR 12 TC 2 Z9 2 U1 1 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0893-0341 J9 ALZ DIS ASSOC DIS JI Alzheimer Dis. Assoc. Dis. PY 1991 VL 5 SU 1 BP S1 EP S6 DI 10.1097/00002093-199100051-00002 PG 6 WC Clinical Neurology; Pathology SC Neurosciences & Neurology; Pathology GA GU428 UT WOS:A1991GU42800002 PM 1664213 ER PT J AU KRAGEL, AH ROBERTS, WC AF KRAGEL, AH ROBERTS, WC TI COMPOSITION OF ATHEROSCLEROTIC PLAQUES IN THE CORONARY-ARTERIES IN HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA SO AMERICAN HEART JOURNAL LA English DT Note RP KRAGEL, AH (reprint author), NHLBI,PATHOL BRANCH,BLDG 10,ROOM 2N258,BETHESDA,MD 20892, USA. NR 5 TC 13 Z9 13 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-8703 J9 AM HEART J JI Am. Heart J. PD JAN PY 1991 VL 121 IS 1 BP 210 EP 211 DI 10.1016/0002-8703(91)90982-N PN 1 PG 2 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA EQ993 UT WOS:A1991EQ99300035 PM 1985370 ER PT J AU ANDERSON, KM ODELL, PM WILSON, PWF KANNEL, WB AF ANDERSON, KM ODELL, PM WILSON, PWF KANNEL, WB TI CARDIOVASCULAR-DISEASE RISK PROFILES SO AMERICAN HEART JOURNAL LA English DT Article; Proceedings Paper CT SYMP ON MANAGEMENT OF CORONARY HEART DISEASE RISK FACTORS IN HYPERTENSIVE PATIENTS : CLINICAL EXPERIENCE WITH DOXAZOSIN CY JAN 26, 1990 CL LONDON, ENGLAND SP PFIZER INT AB This article presents prediction equations for several cardiovascular disease endpoints, which are based on measurements of several known risk factors. Subjects (n = 5573) were original and offspring subjects in the Framingham Heart Study, aged 30 to 74 years, and initially free of cardiovascular disease. Equations to predict risk for the following were developed: myocardial infarction, coronary heart disease (CHD), death from CHD, stroke, cardiovascular disease, and death from cardiovascular disease. The equations demonstrated the potential importance of controlling multiple risk factors (blood pressure, total cholesterol, high-density lipoprotein cholesterol, smoking, glucose intolerance, and left ventricular hypertrophy) as opposed to focusing on one single risk factor. The parametric model used was seen to have several advantages over existing standard regression models. Unlike logistic regression, it can provide predictions for different lengths of time, and probabilities can be expressed in a more straightforward way than the Cox proportional hazards model. C1 NHLBI,BETHESDA,MD 20892. BOSTON UNIV,BOSTON,MA 02215. NR 6 TC 1176 Z9 1189 U1 1 U2 53 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-8703 J9 AM HEART J JI Am. Heart J. PD JAN PY 1991 VL 121 IS 1 SU S BP 293 EP 298 DI 10.1016/0002-8703(91)90861-B PN 2 PG 6 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA EQ995 UT WOS:A1991EQ99500009 PM 1985385 ER PT J AU GOLDBERG, RJ BENGTSON, J CHEN, Z ANDERSON, KM LOCATI, E LEVY, D AF GOLDBERG, RJ BENGTSON, J CHEN, Z ANDERSON, KM LOCATI, E LEVY, D TI DURATION OF THE QT INTERVAL AND TOTAL AND CARDIOVASCULAR MORTALITY IN HEALTHY-PERSONS (THE FRAMINGHAM HEART-STUDY EXPERIENCE) SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID ACUTE MYOCARDIAL-INFARCTION; VENTRICULAR-FIBRILLATION; SUDDEN-DEATH; LONG QT; PROLONGATION; ARRHYTHMIAS; DISEASE AB The baseline electrocardiograms of 5,125 original subjects of the Framingham Heart Study were measured to examine the relation of the QT interval corrected for heart rate (QTc) t risk of total mortality, sudden cardiac death, and death due to coronary artery disease over a 30-year follow-up period. Quintiles of QTc (seconds) less-than-or-equal-to 0.36, 0.36 to 0.38, 0.39 to 0.40, 0.41 to 0.43 and greater-than-or-equal-to 0.44 were studied in relation to these outcomes. There were no significant differences in the risk of total mortality, sudden cardiac death or death due to coronary artery disease according to QTc. A similar lack of significant association between QTc and these 3 outcomes was observed among all persons studied and in the 2 sexes after using a multiple regression analysis to control for several potentially confounding characteristics including age, gender, cigarette smoking, serum total cholesterol, systolic systemic blood pressure and Framingham relative weight. The results of this study fail to demonstrate an association between baseline QTc and overall mortality, and deaths due to sudden cardiac events or coronary artery disease in a large population-based cohort of essentially healthy persons in whom pathologic forms of QTc prolongation are uncommon. C1 DUKE UNIV,DEPT MED,DURHAM,NC 27706. NHLBI,BETHESDA,MD 20892. UNIV MILAN,MED 1 CLIN,I-20122 MILAN,ITALY. RP GOLDBERG, RJ (reprint author), UNIV MASSACHUSETTS,SCH MED,DEPT MED,55 LAKE AVE N,WORCESTER,MA 01655, USA. NR 22 TC 134 Z9 136 U1 1 U2 2 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD JAN 1 PY 1991 VL 67 IS 1 BP 55 EP 58 DI 10.1016/0002-9149(91)90099-7 PG 4 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA EQ819 UT WOS:A1991EQ81900011 PM 1986505 ER PT J AU BLOCK, G AF BLOCK, G TI VITAMIN-C AND CANCER PREVENTION - THE EPIDEMIOLOGIC EVIDENCE SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article; Proceedings Paper CT CONF ON ANTIOXIDANT VITAMINS AND BETA-CAROTENE IN DISEASE PREVENTION CY OCT 02-04, 1989 CL LONDON, ENGLAND DE ASCORBIC ACID; VITAMIN-C; EPIDEMIOLOGIC STUDIES; EPITHELIAL CANCERS; CANCER; REVIEW ID COLO-RECTAL CANCER; UTERINE CERVICAL DYSPLASIA; EPITHELIAL OVARIAN-CANCER; PAST MEDICAL HISTORY; HUMAN-BLOOD PLASMA; ASCORBIC-ACID; LUNG-CANCER; DIETARY FACTORS; COLORECTAL-CANCER; STOMACH-CANCER AB Epidemiologic evidence of a protective effect of vitamin C for non-hormone-dependent cancers is strong. Of the 46 such studies in which a dietary vitamin C index was calculated, 33 found statistically significant protection, with high intake conferring approximately a twofold protective effect compared with low intake. Of 29 additional studies that assessed fruit intake, 21 found significant protection. For cancers of the esophagus, larynx, oral cavity, and pancreas, evidence for a protective effect of vitamin C or some component in fruit is strong and consistent. For cancers of the stomach, rectum, breast, and cervix there is also strong evidence. Several recent lung cancer studies found significant protective effects of vitamin C or of foods that are better sources of vitamin C than of beta-carotene. It is likely that ascorbic acid, carotenoids, and other factors in fruits and vegetables act jointly. Increased consumption of fruits and vegetables in general should be encouraged. RP BLOCK, G (reprint author), NCI,DIV CANC PREVENT & CONTROL,EPN,ROOM 313,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Block, Gladys/E-3304-2010 NR 154 TC 203 Z9 205 U1 5 U2 15 PU AMER SOC CLIN NUTRITION INC PI BETHESDA PA 9650 ROCKVILLE PIKE SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JAN PY 1991 VL 53 IS 1 SU S BP S270 EP S282 PG 13 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ER128 UT WOS:A1991ER12800015 PM 1985398 ER PT J AU BLOCK, G AF BLOCK, G TI DIETARY GUIDELINES AND THE RESULTS OF FOOD-CONSUMPTION SURVEYS SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article; Proceedings Paper CT CONF ON ANTIOXIDANT VITAMINS AND BETA-CAROTENE IN DISEASE PREVENTION CY OCT 02-04, 1989 CL LONDON, ENGLAND DE DIET; SURVEYS; BETA-CAROTENE; VITAMIN-C AB This review focuses on dietary guidelines regarding fruit and vegetable consumption; sources of the antioxidant micronutrients, beta-carotene and vitamin C; and survey data on the US population's actual consumption of these foods. The Dietary Guidelines for Americans recommended consumption of five or more servings of vegetables and fruits daily, as did the 1989 Diet and Health report, which also emphasized fruits and vegetables rich in vitamin C and carotenoids. In contrast with these recommendations and strong evidence for a beneficial effect, very few individuals in the United States even approach the recommended levels of intake. In the US NHANES II data, 41% of the population had a fruit or vegetables rich in vitamin A or in vitamin C. Only 10% consumed the recommended five servings. Efforts to increase consumption of these foods are needed to improve the diet and health of the population. RP BLOCK, G (reprint author), NCI,DIV CANC PREVENT & CONTROL,EPN,RM 313,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Block, Gladys/E-3304-2010 NR 9 TC 37 Z9 37 U1 0 U2 0 PU AMER SOC CLIN NUTRITION INC PI BETHESDA PA 9650 ROCKVILLE PIKE SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JAN PY 1991 VL 53 IS 1 SU S BP S356 EP S357 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ER128 UT WOS:A1991ER12800027 PM 1985410 ER PT J AU CUTLER, RG AF CUTLER, RG TI ANTIOXIDANTS AND AGING SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article; Proceedings Paper CT CONF ON ANTIOXIDANT VITAMINS AND BETA-CAROTENE IN DISEASE PREVENTION CY OCT 02-04, 1989 CL LONDON, ENGLAND DE AGING; ANTIOXIDANTS; LIFE SPAN; CAROTENOIDS; RETINOIDS; URIC ACID; FREE RADICALS ID MOUSE STRAIN; LIFE-SPAN; LONGEVITY; AGE; EVOLUTION; COMPLEXITY; EXPRESSION; BRAIN; LIVER; MICE AB Aging in mammalian species appears to be the result of normal developmental and metabolic processes. In spite of the vast complexity of aging processes, relatively less complex processes such as longevity determinant genes (LDGs) may exist governing aging rate. Much experimental data exists indicating a causative role of oxyradicals in aging processes. In testing the hypothesis that antioxidants may represent LDGs, a positive correlation in the tissue concentration of specific antioxidants with life span of mammals was found. These antioxidants include superoxide dismutase, carotenoids, alpha-tocopherol, and uric acid. We also found that the resistance of tissues to spontaneous autoxidation and the amount of oxidative damage to DNA correlates inversely with life span of mammals. These results suggest a role of oxyradicals in causing aging and that the antioxidant status of an individual could be important in determining frequency of age-dependent diseases and duration of general health maintenance. RP CUTLER, RG (reprint author), NIA,GERONTOL RES CTR,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 27 TC 122 Z9 125 U1 1 U2 4 PU AMER SOC CLIN NUTRITION INC PI BETHESDA PA 9650 ROCKVILLE PIKE SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JAN PY 1991 VL 53 IS 1 SU S BP S373 EP S379 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ER128 UT WOS:A1991ER12800031 PM 1985414 ER PT J AU MALONE, WF AF MALONE, WF TI STUDIES EVALUATING ANTIOXIDANTS AND BETA-CAROTENE AS CHEMOPREVENTIVES SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article; Proceedings Paper CT CONF ON ANTIOXIDANT VITAMINS AND BETA-CAROTENE IN DISEASE PREVENTION CY OCT 02-04, 1989 CL LONDON, ENGLAND DE CHEMOPREVENTION; HUMAN EFFICACY STUDY; INHIBITORY AGENT; BETA-CAROTENE ID PERFORMANCE LIQUID-CHROMATOGRAPHY; CANCER PREVENTION; PLASMA-LEVELS; MUCOSA CELLS; RISK; SERUM; SEPARATION AB Cancer chemoprevention research takes leads from epidemiologic and laboratory research and develops them through in vitro and in vivo preclinical research and initial human studies into randomized controlled clinical trials. At present, the chemoprevention program is sponsoring 21 human efficacy studies. These trials are testing the potential of agents (beta-carotene, folic acid, 13-cis retinoic acid, 4-hydroxyphenyl retinamide, vitamins C and E, and minerals) as inhibitors of a variety of cancers in humans (colon, lung, esophagus, cervix, bladder, and skin). Endpoints in these studies include overall incidence of cancer, incidence of specific cancers, rate of regression or progression of preneoplastic changes, and changes in cellular or biochemical parameters. Study participants include volunteers from the general population; populations at high risk for cancer because of occupation, lifestyle, or place of residence; persons with previously treated cancers; and persons with preneoplastic lesions. Study designs include single agent randomization, combination of agents and complete factorial designs. RP MALONE, WF (reprint author), NCI,CHEMOPREVENT BRANCH,BETHESDA,MD 20892, USA. NR 21 TC 70 Z9 69 U1 2 U2 3 PU AMER SOC CLIN NUTRITION INC PI BETHESDA PA 9650 ROCKVILLE PIKE SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JAN PY 1991 VL 53 IS 1 SU S BP S305 EP S313 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ER128 UT WOS:A1991ER12800020 PM 1985403 ER PT J AU ZIEGLER, RG AF ZIEGLER, RG TI VEGETABLES, FRUITS, AND CAROTENOIDS AND THE RISK OF CANCER SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article; Proceedings Paper CT CONF ON ANTIOXIDANT VITAMINS AND BETA-CAROTENE IN DISEASE PREVENTION CY OCT 02-04, 1989 CL LONDON, ENGLAND DE BETA-CAROTENE; CAROTENOIDS; CANCER; FRUIT; LUNG; VEGETABLES; VITAMIN-A ID DIETARY VITAMIN-A; SERUM BETA-CAROTENE; MIDDLE-AGED MEN; LUNG-CANCER; SUBSEQUENT RISK; SEROLOGIC PRECURSORS; CIGARETTE-SMOKING; ALPHA-TOCOPHEROL; PLASMA RETINOL; BREAST-CANCER AB Low intake of vegetables, fruits, and carotenoids is consistently associated with increased risk of lung cancer in both prospective and retrospective studies. In addition, low levels of beta-carotene in serum or plasma are consistently associated with the subsequent development of lung cancer. The simplest explanation is that beta-carotene is protective. Since retinol (preformed vitamin A) is not related in a similar manner to lung cancer risk, beta-carotene appears to function through a mechanism that does not require conversion into vitamin A. However, the importance of other carotenoids and other constituents of vegetables and fruit had not been adequately explored. Both prospective and retrospective studies suggest that vegetable and fruit intake may reduce the risk of cancers of the mouth, pharynx, larynx, esophagus, stomach, colon, rectum, bladder, and cervix. But because of fewer studies and less consistency among studies, the epidemiologic evidence is at present less persuasive than for lung cancer. RP ZIEGLER, RG (reprint author), NCI,DIV CANC ETIOL,EPIDEMIOL & BIOSTAT PROGRAM,BETHESDA,MD 20892, USA. NR 46 TC 214 Z9 218 U1 1 U2 3 PU AMER SOC CLIN NUTRITION INC PI BETHESDA PA 9650 ROCKVILLE PIKE SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JAN PY 1991 VL 53 IS 1 SU S BP S251 EP S259 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ER128 UT WOS:A1991ER12800012 PM 1985395 ER PT J AU JAFFE, ES ABBONDANZO, SL AF JAFFE, ES ABBONDANZO, SL TI CD30 ANTIGEN EXPRESSION SO AMERICAN JOURNAL OF CLINICAL PATHOLOGY LA English DT Letter C1 ARMED FORCES INST PATHOL,DEPT HEMATOL & LYMPHAT PATHOL,WASHINGTON,DC 20306. RP JAFFE, ES (reprint author), NCI,HEMATOPATHOL SECT,PATHOL LAB,BETHESDA,MD 20892, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0002-9173 J9 AM J CLIN PATHOL JI Am. J. Clin. Pathol. PD JAN PY 1991 VL 95 IS 1 BP 103 EP 103 PG 1 WC Pathology SC Pathology GA EQ528 UT WOS:A1991EQ52800019 ER PT J AU WEDDINGTON, WW BROWN, BS HAERTZEN, CA HESS, JM MAHAFFEY, JR KOLAR, AF JAFFE, JH AF WEDDINGTON, WW BROWN, BS HAERTZEN, CA HESS, JM MAHAFFEY, JR KOLAR, AF JAFFE, JH TI COMPARISON OF AMANTADINE AND DESIPRAMINE COMBINED WITH PSYCHOTHERAPY FOR TREATMENT OF COCAINE DEPENDENCE SO AMERICAN JOURNAL OF DRUG AND ALCOHOL ABUSE LA English DT Article; Proceedings Paper CT 1989 ANNUAL MEETING OF THE COMMITTEE ON PROBLEMS OF DRUG DEPENDENCE CY JUN 19, 1989 CL KEYSTONE, CO SP COMM PROBLEM DRUG DEPENDENCE ID CORTICAL DOPAMINERGIC INVOLVEMENT; PLASMA-CONCENTRATION; SELF-STIMULATION; AMPHETAMINE; DEPRESSION; ABUSE; BRAIN; REINFORCEMENT; RELIABILITY; ADULTS C1 NIDA,ADDICT RES CTR,BALTIMORE,MD 21224. NR 42 TC 97 Z9 98 U1 1 U2 1 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0095-2990 J9 AM J DRUG ALCOHOL AB JI Am. J. Drug Alcohol Abuse PY 1991 VL 17 IS 2 BP 137 EP 152 DI 10.3109/00952999108992817 PG 16 WC Psychology, Clinical; Substance Abuse SC Psychology; Substance Abuse GA FL811 UT WOS:A1991FL81100003 PM 1862788 ER PT J AU WEDDINGTON, WW HAERTZEN, CA HESS, JM BROWN, BS AF WEDDINGTON, WW HAERTZEN, CA HESS, JM BROWN, BS TI PSYCHOLOGICAL REACTIONS AND RETENTION BY COCAINE ADDICTS DURING TREATMENT ACCORDING TO HIV-SEROSTATUS - A MATCHED-CONTROL STUDY SO AMERICAN JOURNAL OF DRUG AND ALCOHOL ABUSE LA English DT Article ID ACQUIRED IMMUNODEFICIENCY SYNDROME; ABUSE; ACCEPTANCE; AIDS C1 NIDA,CTR ADDICT RES,BALTIMORE,MD 21224. NIDA,ROCKVILLE,MD 20857. NR 24 TC 9 Z9 9 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0095-2990 J9 AM J DRUG ALCOHOL AB JI Am. J. Drug Alcohol Abuse PY 1991 VL 17 IS 3 BP 355 EP 368 PG 14 WC Psychology, Clinical; Substance Abuse SC Psychology; Substance Abuse GA GB169 UT WOS:A1991GB16900010 PM 1928028 ER PT J AU KOTLOFF, KL TACKET, CO CLEMENS, JD WASSERMAN, SS COWAN, JE BRIDWELL, MW QUINN, TC AF KOTLOFF, KL TACKET, CO CLEMENS, JD WASSERMAN, SS COWAN, JE BRIDWELL, MW QUINN, TC TI ASSESSMENT OF THE PREVALENCE AND RISK-FACTORS FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) INFECTION AMONG COLLEGE-STUDENTS USING 3 SURVEY METHODS SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE ACQUIRED IMMUNODEFICIENCY SYNDROME; HIV; HIV SEROPOSITIVITY; SEX BEHAVIOR; SEXUAL PARTNERS; STUDENTS ID WOMEN AB To evaluate the seroprevalence and risk factors for human immunodeficiency virus type 1 (HIV-1) infection among undergraduate college students, the authors simultaneously conducted three types of surveillance on a large university campus (27,902 undergraduates) in the Baltimore-Washington metropolitan area: a voluntary HIV-1 serosurvey with a linked risk assessment questionnaire (n = 3,394), a blinded serosurvey using blood specimens collected for routine purposes in the Student Health Center (n = 1,829), and a random sample risk assessment and case identification mail survey (n = 1,017 respondents of 3,000 solicited). The proportion of students belonging to a known risk group (a homosexual or bisexual man, intravenous drug user, or a sexual partner of a bisexual man, an HIV-1-infected person, a female prostitute, or an intravenous drug user) was 5.9% in the mail survey and 8.8% in the voluntary serosurvey. Whereas no infections were detected in the blinded serosurvey, two infected persons were identified in the mail survey (0.2%) and two in the voluntary serosurvey (0.06%), all among high-risk persons. Although derived from independent samples and subject to different biases, these three survey methods yielded a consistent pattern of HIV-1 epidemiology on this campus, whereby the overall prevalence of infection was low and confined to members of high-risk groups, despite the common occurrence of behaviors that might facilitate sexual transmission of HIV-1 among many other students. C1 UNIV MARYLAND,SCH MED,CTR VACCINE DEV,DIV GEOG MED,BALTIMORE,MD 21201. UNIV MARYLAND,CTR HLTH,COLLEGE PK,MD 20742. NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. RP KOTLOFF, KL (reprint author), UNIV MARYLAND,SCH MED,CTR VACCINE DEV,DIV INFECT DIS & TROP PEDIAT,10 S PINE ST,BALTIMORE,MD 21201, USA. RI kotloff, karen/E-7768-2012 OI kotloff, karen/0000-0003-1808-6431 FU NIAID NIH HHS [AI-28711] NR 19 TC 18 Z9 18 U1 0 U2 2 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JAN 1 PY 1991 VL 133 IS 1 BP 2 EP 8 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EP704 UT WOS:A1991EP70400002 PM 1983894 ER PT J AU VANHORN, LV BALLEW, C LIU, K RUTH, K MCDONALD, A HILNER, JE BURKE, GL SAVAGE, PJ BRAGG, C CAAN, B JACOBS, D SLATTERY, M SIDNEY, S AF VANHORN, LV BALLEW, C LIU, K RUTH, K MCDONALD, A HILNER, JE BURKE, GL SAVAGE, PJ BRAGG, C CAAN, B JACOBS, D SLATTERY, M SIDNEY, S TI DIET, BODY SIZE, AND PLASMA LIPIDS-LIPOPROTEINS IN YOUNG-ADULTS - DIFFERENCES BY RACE AND SEX - THE CORONARY-ARTERY RISK DEVELOPMENT IN YOUNG-ADULTS (CARDIA) STUDY SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE ADULT; BODY MASS INDEX; CHOLESTEROL; DIET; LIPOPROTEINS; HDL CHOLESTEROL; LIPOPROTEINS, LDL CHOLESTEROL ID MONOUNSATURATED FATTY-ACIDS; SERUM-CHOLESTEROL; HEART-DISEASE; MEN; PRECIPITATION; QUANTITATION; ASSOCIATION; DESIGN AB The Coronary Artery Risk Development in Young Adults (CARDIA) study completed baseline dietary assessment, measurement of body mass index, and lipid and lipoprotein analyses on 5,111 participants. CARDIA includes black and white men and women between 18 and 30 years of age at baseline (1985-1986), recruited in Birmingham, Alabama, Chicago, Illinois, Minneapolis, Minnesota, and Oakland, California. Diet was assessed by a detailed interviewer-administered diet history that measured the usual eating pattern over the past month. Possible sex, race, age, and educational differences in diet, body size, and lipids-lipoproteins were explored. Nutrient analyses indicate that the Keys score, a measure of dietary fat composition, is significantly correlated with plasma cholesterol in older (aged 25-30 years) white men (r = 0.12, p < 0.01) and older white women (r = 0.12, p < 0.001). In multiple linear regression analyses, body mass index was positively and significantly associated with total cholesterol and low density lipoprotein cholesterol and inversely associated with high density lipoprotein cholesterol across all race-sex groups. The Keys score was significantly associated with total cholesterol and low density lipoprotein cholesterol in white men and women. Education was associated with high density lipoprotein cholesterol in black and white women and white men. Education was associated with high density lipoprotein cholesterol in black and white women and white men. In these young adults, dietary fat intake and body mass index were related to blood lipids in certain subgroups. In black and white men and black women, blood cholesterol increased with age across race-sex groups independently of these covariates. In view of the many factors affecting plasma cholesterol and the limitations of the dietary history method, these cross-sectional data are useful in characterizing diet and lipid differences. There appears to be general disparity between recommended dietary intake of total fat, saturated fat, and other nutrients and actual dietary intake in young adults, regardless of age and educational level. C1 UNIV ALABAMA,BIRMINGHAM,AL 35294. UNIV MINNESOTA,MINNEAPOLIS,MN 55455. NHLBI,BETHESDA,MD 20892. UNIV UTAH,SALT LAKE CITY,UT 84112. KAISER PERMANENTE,OAKLAND,CA. RP VANHORN, LV (reprint author), NORTHWESTERN UNIV,SCH MED,DEPT COMMUNITY HLTH & PREVENT MED,680 N LAKE SHORE DR,SUITE 1102,CHICAGO,IL 60611, USA. NR 44 TC 63 Z9 63 U1 0 U2 0 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JAN 1 PY 1991 VL 133 IS 1 BP 9 EP 23 PG 15 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EP704 UT WOS:A1991EP70400003 PM 1983903 ER PT J AU SHAW, GL TUCKER, MA KASE, RG HOOVER, RN AF SHAW, GL TUCKER, MA KASE, RG HOOVER, RN TI PROBLEMS ASCERTAINING FRIEND CONTROLS IN A CASE-CONTROL STUDY OF LUNG-CANCER SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Note DE CASE-CONTROL STUDIES; EPIDEMIOLOGIC METHODS; LUNG NEOPLASMS; RESEARCH DESIGN ID TOXIC-SHOCK SYNDROME; ASSOCIATION AB Willingness of newly diagnosed lung cancer patients to volunteer names of friends as potential control subjects was assessed from August through December 1988 in a case-control study at the National Cancer Institute and Naval Hospital, Bethesda, Maryland. Friend controls appeared ideal to examine a genetically determined metabolic characteristic and lung cancer risk, since potentially confounding characteristics could be matched and cooperation should be high. Only 11 of 23 cases named at least one friend. Cases interviewed during the second study month were most likely to volunteer names. Either the recency of the diagnosis of a highly fatal disease or the referral to a tertiary care research hospital may have contributed to the reluctance to cases ot volunteer names. No characteristic was identified that might offer a means to increase referral of friend controls. RP SHAW, GL (reprint author), NCI,DIV CANC ETIOL,ENVIRONM EPIDEMIOL BRANCH,EXECUT PLAZA N,SUITE 439,BETHESDA,MD 20892, USA. RI Tucker, Margaret/B-4297-2015 NR 6 TC 9 Z9 9 U1 0 U2 0 PU AMER J EPIDEMIOLOGY PI BALTIMORE PA 624 N BROADWAY RM 225, BALTIMORE, MD 21205 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JAN 1 PY 1991 VL 133 IS 1 BP 63 EP 66 PG 4 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EP704 UT WOS:A1991EP70400009 PM 1845760 ER PT J AU BERG, PE MITTELMAN, M ELION, J LABIE, D SCHECHTER, AN AF BERG, PE MITTELMAN, M ELION, J LABIE, D SCHECHTER, AN TI INCREASED PROTEIN-BINDING TO A-530 MUTATION OF THE HUMAN BETA-GLOBIN GENE ASSOCIATED WITH DECREASED BETA-GLOBIN SYNTHESIS SO AMERICAN JOURNAL OF HEMATOLOGY LA English DT Article DE BETA-GLOBIN GENE; THALASSEMIA; MUTATION; REPRESSOR ID TRANSGENIC MICE; CELL-LINE; THALASSEMIA; TRANSCRIPTION; EXPRESSION; SEQUENCES; PROMOTER; SILENCERS; CONTAINS; REGIONS AB Although some cases of the syndrome of hereditary persistence of fetal hemoglobin (HPFH) have been correlated with mutations causing a change in the binding of trans-acting factors to DNA sequences flanking the gamma-globulin gene, this mechanism has not been described in beta-thalassemias upstream of the canonical promoter of the beta-globin gene. In this report we describe such a change in binding of a protein that may explain a silent carrier phenotype of beta-thalassemia. We have previously demonstrated the binding of a protein (BP1) derived from a nuclear extract of human K562 cells to DNA 5' to the human beta-globin gene in a region having a negative regulatory function. The binding of BP1 in this region can be detected by DNAse I footprinting and by gel mobility shift analysis. We have now compared binding of BP1 to the normal sequence and a mutated sequence (+ATA/-T at 1530 bp from the cap site) from the silent carrier of beta-thalassemia. Using mobility shift assays we show that BP1 binds about nine times more strongly to the mutated sequence than the normal sequence. These results suggest the possibility that the decreased expression of the beta-globin gene exhibited by the carrier may be due, at least in part, to tighter binding of a protein which functions as a negative control element or repressor. C1 HOP ROBERT DEBRE,PARIS,FRANCE. INSERM,UNITE 15,F-75005 PARIS,FRANCE. RP BERG, PE (reprint author), NIDDK,CHEM BIOL LAB,BLDG 10,ROOM 9N-312,BETHESDA,MD 20892, USA. RI Elion, Jacques/A-1048-2014; OI Schechter, Alan N/0000-0002-5235-9408 NR 34 TC 54 Z9 54 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0361-8609 J9 AM J HEMATOL JI Am. J. Hematol. PD JAN PY 1991 VL 36 IS 1 BP 42 EP 47 DI 10.1002/ajh.2830360109 PG 6 WC Hematology SC Hematology GA EP257 UT WOS:A1991EP25700008 PM 1984681 ER PT J AU WADLEIGH, RG CHAN, CS COHEN, MH KRASNOW, SH ANDERSON, A DETERAWADLEIGH, SD AF WADLEIGH, RG CHAN, CS COHEN, MH KRASNOW, SH ANDERSON, A DETERAWADLEIGH, SD TI H-RAS-1 POLYMORPHISM IN LEUKEMIA SO AMERICAN JOURNAL OF HEMATOLOGY LA English DT Article DE SACL POLYMORPHISM; B-CELL LEUKEMIA; ACUTE MYELOGENOUS LEUKEMIA ID REGION GENES; FRAGMENTS; MUTATIONS; LOCUS AB We have examined leukocyte DNAs obtained from 21 patients with various types of leukemia for the Sacl polymorphism of H-ras-1. The patients included ten with B-lineage leukemia, two with T-cell leukemia, and nine with acute myelogenous leukemia. Using a genomic probe for H-ras-1 in Southern hybridizations, we found that 78% of the DNA from leukemias of myeloid origin were heterozygous. In contrast, only three of ten B-cell-derived leukemias showed heterozygosity. This difference in the number of heterozygotes vs. homozygotes in the two patient samples studied is statistically significant (P = 0.04 by Fisher's exact test). These results suggest the possibility that the H-ras-1 allelic pattern that is associated with acute myelogenous leukemia is distinct from that found in B-cell-derived malignancies. C1 VET ADM MED CTR,HEMATOL SECT,WASHINGTON,DC 20422. NIMH,CLIN NEUROGENET BRANCH,BETHESDA,MD 20892. RP WADLEIGH, RG (reprint author), VET ADM MED CTR,MED ONCOL SECT,2 CN,50 IRVING ST NW,WASHINGTON,DC 20422, USA. NR 10 TC 0 Z9 0 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0361-8609 J9 AM J HEMATOL JI Am. J. Hematol. PD JAN PY 1991 VL 36 IS 1 BP 69 EP 70 DI 10.1002/ajh.2830360117 PG 2 WC Hematology SC Hematology GA EP257 UT WOS:A1991EP25700016 PM 1670614 ER PT J AU LAUNER, LJ VILLAR, J KESTLER, E AF LAUNER, LJ VILLAR, J KESTLER, E TI EPIDEMIOLOGIC DIFFERENCES AMONG BIRTH-WEIGHT AND GESTATIONAL-AGE SUBGROUPS OF NEWBORNS SO AMERICAN JOURNAL OF HUMAN BIOLOGY LA English DT Article ID FETAL GROWTH; PERINATAL DATA; INFANTS; PROPORTIONALITY; PREMATURITY; RETARDATION; PREGNANCY C1 GUATEMALAN SOCIAL SECUR INST,HOSP GYNECOL & OBSTET,PERINATAL RES PROGRAM,GUATEMALA CITY,GUATEMALA. RP LAUNER, LJ (reprint author), NICHHD,DIV PREVENT RES,BETHESDA,MD 20892, USA. NR 27 TC 3 Z9 3 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 1042-0533 J9 AM J HUM BIOL JI Am. J. Hum. Biol. PY 1991 VL 3 IS 5 BP 425 EP 433 DI 10.1002/ajhb.1310030503 PG 9 WC Anthropology; Biology SC Anthropology; Life Sciences & Biomedicine - Other Topics GA GJ395 UT WOS:A1991GJ39500002 ER PT J AU PROVINCE, MA KELLER, J HIGGINS, M RAO, DC AF PROVINCE, MA KELLER, J HIGGINS, M RAO, DC TI A COMMINGLING ANALYSIS OF OBESITY IN THE TECUMSEH COMMUNITY-HEALTH STUDY SO AMERICAN JOURNAL OF HUMAN BIOLOGY LA English DT Article ID GENETIC-ANALYSIS; FAMILIAL AGGREGATION; ENVIRONMENT; WEIGHT; RESEMBLANCE; CHILDREN; HEREDITY; HEIGHT; TWIN C1 NHLBI,BETHESDA,MD 20892. RP PROVINCE, MA (reprint author), WASHINGTON UNIV,SCH MED,DIV BIOSTAT,BOX 806,600 S EUCLID ST,ST LOUIS,MO 63110, USA. NR 30 TC 2 Z9 2 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 1042-0533 J9 AM J HUM BIOL JI Am. J. Hum. Biol. PY 1991 VL 3 IS 5 BP 435 EP 445 DI 10.1002/ajhb.1310030504 PG 11 WC Anthropology; Biology SC Anthropology; Life Sciences & Biomedicine - Other Topics GA GJ395 UT WOS:A1991GJ39500003 ER PT J AU RICE, T PROVINCE, MA KELLER, JB BOUCHARD, C HIGGINS, MW RAO, DC AF RICE, T PROVINCE, MA KELLER, JB BOUCHARD, C HIGGINS, MW RAO, DC TI HETEROGENEITY AMONG POPULATIONS FOR FAMILIAL AGGREGATION OF BLOOD-PRESSURE SO AMERICAN JOURNAL OF HUMAN BIOLOGY LA English DT Article ID FRENCH-CANADIAN POPULATION; CARDIOVASCULAR RISK-FACTORS; EXTENSIVE INFORMATION; RESOLUTION; TECUMSEH; LIPIDS C1 WASHINGTON UNIV,SCH MED,DEPT PSYCHIAT,ST LOUIS,MO 63110. WASHINGTON UNIV,SCH MED,DEPT GENET,ST LOUIS,MO 63110. NHLBI,DEPT EPIDEMIOL & BIOMETRY,BETHESDA,MD 20892. LAVAL UNIV,PHYS ACTIV SCI LAB,ST FOY,QUEBEC,CANADA. RP RICE, T (reprint author), WASHINGTON UNIV,SCH MED,DIV BIOSTAT,ST LOUIS,MO 63110, USA. RI Rice, Treva/D-1385-2009; Bouchard, Claude/A-7637-2009 NR 15 TC 5 Z9 5 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 1042-0533 J9 AM J HUM BIOL JI Am. J. Hum. Biol. PY 1991 VL 3 IS 5 BP 515 EP 523 DI 10.1002/ajhb.1310030511 PG 9 WC Anthropology; Biology SC Anthropology; Life Sciences & Biomedicine - Other Topics GA GJ395 UT WOS:A1991GJ39500010 ER PT J AU FINKELSTEIN, JE DOEGE, K YAMADA, Y PYERITZ, RE GRAHAM, JM MOESCHLER, JB PAULI, RM HECHT, JT FRANCOMANO, CA AF FINKELSTEIN, JE DOEGE, K YAMADA, Y PYERITZ, RE GRAHAM, JM MOESCHLER, JB PAULI, RM HECHT, JT FRANCOMANO, CA TI ANALYSIS OF THE CHONDROITIN SULFATE PROTEOGLYCAN CORE PROTEIN (CSPGCP) GENE IN ACHONDROPLASIA AND PSEUDOACHONDROPLASIA SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Article ID PRENATAL-DIAGNOSIS; COMPRESSION; APNEA; DNA AB Achondroplasia and pseudoachondroplasia are autosomal dominant skeletal dysplasias resulting in short-limbed dwarfism. Histologic and ultrastructural studies of the cartilage in pseudoachondroplasia and in homozygous achondroplasia have suggested a structural abnormality in chondroitin sulfate proteoglycan (CSPG), a major structural protein in the extra-cellular matrix. The gene encoding CSPG core protein (CSPGCP) is thus a logical "candidate gene" for analysis in these conditions. cDNA probes encoding CSPGCP were used to identify restriction fragment length polymorphisms (RFLPs) in DNA from a panel of control individuals. No gross alterations at the CSPGCP locus were noted in DNA from 37 individuals with achondroplasia and 5 individuals with pseudoachondroplasia. In addition, allelic frequencies of the RFLPs were not significantly different among controls and patients with either condition. In one three-generation family with achondroplasia, close linkage of the CSPGCP locus and the skeletal dysplasia was excluded using a Bgl II polymorphism. Similarly, in a three-generation family with pseudoachondroplasia, the CSPGCP gene was not tightly linked to the disease phenotype. These results indicate that mutations at the chondroitin sulfate proteoglycan core protein locus do not cause achondroplasia or pseudoachondroplasia in these families. C1 JOHNS HOPKINS UNIV,DEPT PEDIAT,BALTIMORE,MD 21218. JOHNS HOPKINS UNIV,DEPT MED,BALTIMORE,MD 21218. NIDR,BETHESDA,MD 20892. UNIV CALIF LOS ANGELES,SCH MED,CEDARS SINAI MED CTR,AHMANSON PEDIAT CTR,LOS ANGELES,CA 90024. DARTMOUTH COLL,HITCHCOCK MED CTR,DARTMOUTH MED SCH,DEPT MATERNAL & CHILD HLTH,HANOVER,NH 03756. UNIV WISCONSIN,DEPT MATERNAL & CHILD HLTH,MADISON,WI 53706. UNIV WISCONSIN,DEPT PEDIAT,MADISON,WI 53706. UNIV WISCONSIN,DEPT MED GENET,MADISON,WI 53706. UNIV TEXAS,SCH MED,DEPT PEDIAT,HOUSTON,TX 77025. RI Pyeritz, Reed/A-1364-2010 FU NIADDK NIH HHS [AM01361]; NIGMS NIH HHS [GM 07471, GM41015-02] NR 24 TC 23 Z9 23 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD JAN PY 1991 VL 48 IS 1 BP 97 EP 102 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA ET188 UT WOS:A1991ET18800013 PM 1670752 ER PT J AU HAYES, RB AF HAYES, RB TI LUNG-CANCER IN MOTOR EXHAUST-RELATED OCCUPATIONS SO AMERICAN JOURNAL OF INDUSTRIAL MEDICINE LA English DT Letter RP HAYES, RB (reprint author), NCI,ENVIRONM EPIDEMIOL BRANCH,EPN ROOM 418,BETHESDA,MD 20892, USA. NR 1 TC 3 Z9 3 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0271-3586 J9 AM J IND MED JI Am. J. Ind. Med. PD JAN PY 1991 VL 19 IS 1 BP 135 EP 135 DI 10.1002/ajim.4700190116 PG 1 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EL682 UT WOS:A1991EL68200015 PM 1703395 ER PT J AU KLEBANOFF, MA SHIONO, PH SELBY, JV TRACHTENBERG, AI GRAUBARD, BI AF KLEBANOFF, MA SHIONO, PH SELBY, JV TRACHTENBERG, AI GRAUBARD, BI TI ANEMIA AND SPONTANEOUS PRETERM BIRTH SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Article DE ANEMIA; HEMATOCRIT; PREGNANCY; PREMATURE LABOR ID PREGNANCY; DELIVERY AB The association between anemia during pregnancy and spontaneous preterm birth was studied with a two-stage case-control design in a large, multiethnic cohort. Results of all hematologic measurements were abstracted from the prenatal and delivery records of 1706 of the 26,901 women in the cohort. Among women delivered of infants at term, mean hematocrit value was low during the early phase of the second trimester, stable until near term, then reached a maximum at 40 weeks' gestation. The mean hematocrit value of black women was consistently lower than that of Asian, Mexican, and white women. Anemia (hematocrit value less than the tenth percentile for ethnic group and duration of pregnancy) at any time during the second trimester was positively associated with subsequent spontaneous preterm birth (odds ratio, 1.9; 95% confidence interval, 1.3 to 2.8). Compared with white women, the odds ratios for preterm birth were 2.0 (95% confidence interval, 1.6 to 2.4) for black, 1.2 (95% confidence interval, 0.9 to 1.6) for Asian, and 1.2 (95% confidence interval, 1.0 to 1.5) for Mexican women. Adjustment for second-trimester anemia had minimal influence on the odds ratios. We conclude that anemia during the second trimester was associated with preterm birth. However, it does not account for the large ethnic differences in preterm birth. C1 DAVID & LUCILE PACKARD FDN,CTR FUTURE CHILDREN,OAKLAND,CA. BAY AREA ADDICT RES & TREATMENT,DIV RES,PERMANENTE MED GRP,OAKLAND,CA. NCI,DIV CANC PREVENT & CONTROL,BIOMETRY BRANCH,BALTIMORE,MD 21211. RP KLEBANOFF, MA (reprint author), NICHHD,DIV PREVENT RES,EPN 640,BETHESDA,MD 20892, USA. FU NICHD NIH HHS [N01-HD-2861] NR 12 TC 112 Z9 119 U1 1 U2 4 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-9378 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD JAN PY 1991 VL 164 IS 1 BP 59 EP 63 PN 1 PG 5 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA ET500 UT WOS:A1991ET50000014 PM 1986627 ER PT J AU JOVANOVICPETERSON, L PETERSON, CM REED, GF METZGER, BE MILLS, JL KNOPP, RH AARONS, JH AF JOVANOVICPETERSON, L PETERSON, CM REED, GF METZGER, BE MILLS, JL KNOPP, RH AARONS, JH TI MATERNAL POSTPRANDIAL GLUCOSE-LEVELS AND INFANT BIRTH-WEIGHT - THE DIABETES IN EARLY-PREGNANCY STUDY SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Article DE TYPE-I DIABETES MELLITUS; MACROSOMIA; POSTPRANDIAL GLUCOSE; GLYCOSYLATED HEMOGLOBIN; PREGNANCY ID GLYCOSYLATED HEMOGLOBIN; NONDIABETIC WOMEN; FETAL GROWTH; INSULIN; MACROSOMIA; MORBIDITY; PROFILES; MELLITUS; MOTHERS AB The cause of macrosomia in the infant of the diabetic woman is still not completely defined. The National Institute of Child Health and Human Development-Diabetes in Early Pregnancy Study, which recruited insulin-dependent diabetic and control women before conception, provided an opportunity to address the relationship between maternal glycemia and percentile birth weight. Data were analyzed from 323 diabetic and 361 control women. Fasting and nonfasting venous plasma glucose were measured on alternate weeks in the first trimester and monthly thereafter. Glycosylated hemoglobin was measured weekly in the first trimester and monthly thereafter. More infants of the diabetic women were at or above the 90th percentile for birth weight than infants of control women (28.5% versus 13.1%, p < 0.001). Although first-trimester nonfasting glucose and glycosylated hemoglobin levels were positively correlated with infant birth weight (p < 0.001 and p = 0.008), when the analyses were adjusted for the variables of the subsequent trimesters the values became insignificant, whereas the third-trimester nonfasting glucose levels adjusted for values in prior trimesters emerged as the stronger predictor of percentile birth weight (p = 0.001). After adjusting for maternal hypertension, smoking, and ponderal index, the above relationships remained. In conclusion, monitoring of nonfasting glucose levels rather than the fasting levels, which are more commonly monitored in clinical practice, are necessary to prevent macrosomia. C1 NICHHD,BETHESDA,MD 20892. NORTHWESTERN UNIV,CHICAGO,IL 60611. UNIV WASHINGTON,SEATTLE,WA 98195. UNIV PITTSBURGH,PITTSBURGH,PA 15260. RP JOVANOVICPETERSON, L (reprint author), SANSUM MED RES FDN,2219 BATH ST,SANTA BARBARA,CA 93105, USA. FU NCRR NIH HHS [RR00037-28, RR0047] NR 29 TC 240 Z9 248 U1 1 U2 7 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-9378 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD JAN PY 1991 VL 164 IS 1 BP 103 EP 111 PN 1 PG 9 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA ET500 UT WOS:A1991ET50000025 PM 1986596 ER PT J AU EHRENKRANZ, NJ PFAFF, SJ POPPE, D BLACKWELDER, WC YERG, DE KASLOW, RA AF EHRENKRANZ, NJ PFAFF, SJ POPPE, D BLACKWELDER, WC YERG, DE KASLOW, RA TI LOW-RISK CESAREAN-SECTION PROPHYLAXIS ISSUE STILL UNSETTLED - REPLY SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Letter ID MORBIDITY C1 NIAID,WESTWOOD BLDG,ROOM 739,BETHESDA,MD 20892. RP EHRENKRANZ, NJ (reprint author), FLORIDA CONSORTIUM INFECT CONTROL QUAL MED CARE,5901 SW 74TH ST,S MIAMI,FL 33143, USA. NR 9 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-9378 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD JAN PY 1991 VL 164 IS 1 BP 233 EP 234 PN 1 PG 2 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA ET500 UT WOS:A1991ET50000050 ER PT J AU KIRKWOOD, SP ZURLO, F LARSON, K RAVUSSIN, E AF KIRKWOOD, SP ZURLO, F LARSON, K RAVUSSIN, E TI MUSCLE MITOCHONDRIAL MORPHOLOGY, BODY-COMPOSITION, AND ENERGY-EXPENDITURE IN SEDENTARY INDIVIDUALS SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE OBESITY; BASAL METABOLIC RATE; ELECTRON MICROSCOPY; RESPIRATION ID INVIVO INSULIN ACTION; LIMB SKELETAL-MUSCLE; RETICULUM; DETERMINANTS; CAPACITY; EXERCISE; GENETICS; OBESITY AB To investigate whether differences in metabolic rate are related to differences in muscle mitochondrial morphology and/or to differences in in vitro muscle respiration, we studied 17 healthy Causcasians, covering a wide range of body weight and composition [9 males, 8 females; body wt 96 +/- 37 (SD) kg; body fat = 28 +/- 10%]. Central and peripheral mitochondrial volume density (V(mit c) and V(mit p), respectively) and the ratio of mitochondrial outer surface to volume of mitochondria (SV(mit c) in center and SV(mit p) at periphery) were determined by stereological analyses of transmission electron micrographs from samples of the vastus lateralis. There was no relationship between mitochondrial morphology or muscle respiration and 24-h energy expenditure, basal metabolic rate, or sleeping energy expenditure adjusted for differences in fat-free mass, fat mass, age, and sex. Although total body fat was not associated with muscle cell morphology, central distribution of body fat [waist-to-thigh circumference ratio (W/T)] correlated negatively with V(mit c) (r = -0.58, P = 0.01), SV(mit c) (r = -0.59, P = 0.01), and SV(mit p) (r = -0.48, P = 0.05). W/T was also negatively related to muscle respiration (r = -0.59, P = 0.01). Despite the lack of relationship between metabolic rate and muscle mitochondrial morphology, central distribution of body fat is associated with lower mitochondrial density and larger mitochondria in skeletal muscle and is associated with a decreased oxidative capacity of muscle. C1 NIDDKD,CLIN DIABET & NUTR SECT,RM 541,4212 N 16TH ST,PHOENIX,AZ 85016. NR 31 TC 13 Z9 13 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD JAN PY 1991 VL 260 IS 1 BP E89 EP E94 PN 1 PG 6 WC Physiology SC Physiology GA EU065 UT WOS:A1991EU06500035 PM 1987796 ER PT J AU GROSSMAN, E REA, RF HOFFMAN, A GOLDSTEIN, DS AF GROSSMAN, E REA, RF HOFFMAN, A GOLDSTEIN, DS TI YOHIMBINE INCREASES SYMPATHETIC-NERVE ACTIVITY AND NOREPINEPHRINE SPILLOVER IN NORMAL VOLUNTEERS SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE ADRENOCORTICOTROPIC HORMONE; ADRENOCEPTORS; EPINEPHRINE; KINETICS; SYMPATHETIC NERVOUS SYSTEM ID NORADRENALINE RELEASE; ALPHA-ADRENOCEPTORS; TRANSMITTER RELEASE; VASOCONSTRICTION; CATECHOLAMINES; MODULATION; PLASMA; RATS; ALPHA-2-ADRENOCEPTORS; STIMULATION AB It has been difficult to examine clinically the physiological role of central and peripheral alpha-2-adrenoceptors in humans. We simultaneously measured directly recorded peroneal skeletal muscle sympathoneural activity (MSNA) and the rate of appearance (spillover) of norepinephrine (NE) in forearm venous and arterial plasma before and at 15 min during intravenous administration of the alpha-2-blocker yohimbine (Yoh, 125-mu-g/kg bolus, 1-mu-g.kg-1.min-1 infusion) in seven normal volunteers. Yoh administration increased mean arterial pressure by 16% (P < 0.005), heart rate by 8% (P < 0.05), and forearm vascular resistance by 67% (P < 0.05). MSNA was increased by 73% (P < 0.05), NE spillover into arterial blood by 125% (P < 0.05), and forearm NE spillover (FSO) by 337% (P < 0.005). Ganglion blockade by trimethaphan during Yoh infusion decreased MSNA to below detection limits and reversed Yoh-induced increases in arterial concentrations of NE and epinephrine. The results demonstrate that Yoh administration increases sympathoadrenal outflow. Because the mean increase of FSO was much larger than that of MSNA, the results suggest that alpha-2-adrenoceptors on sympathetic nerve endings modulate the neuronal release of NE for a given amount of sympathetic nerve traffic in humans; this effect seems prominent in the human limb. C1 NHLBI,HYPERTENS ENDOCRINE BRANCH,BLDG 10,RM 8C103,BETHESDA,MD 20892. UNIV IOWA,DEPT MED,DIV CARDIOVASC DIS,IOWA CITY,IA 52242. NR 30 TC 65 Z9 65 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD JAN PY 1991 VL 260 IS 1 BP R142 EP R147 PN 2 PG 6 WC Physiology SC Physiology GA EU071 UT WOS:A1991EU07100059 PM 1847020 ER PT J AU KATZ, A BOGARDUS, C AF KATZ, A BOGARDUS, C TI RELATIONSHIP BETWEEN CARBOHYDRATE OXIDATION AND G-1,6-P2 IN HUMAN SKELETAL-MUSCLE DURING EUGLYCEMIC HYPERINSULINEMIA SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE NONINSULIN-DEPENDENT DIABETES MELLITUS; INSULIN RESISTANCE; GLYCOLYSIS; HIGH-ENERGY PHOSPHATES; FRUCTOSE 2,6-BISPHOSPHATE, HEXOSE MONOPHOSPHATES; CATECHOLAMINES; GLYCOGEN SYNTHASE; GLYCOGEN PHOSPHORYLASE; CITRATE ID GLUCOSE 1,6-BISPHOSPHATE; GLYCOGEN-METABOLISM; FRUCTOSE-2,6-BISPHOSPHATE CONTENT; INTRACELLULAR PH; INSULIN; PHOSPHOFRUCTOKINASE; GLYCOLYSIS; PHOSPHORYLATION; ENZYMES; HEARTS AB Euglycemic (approximately 5.5 mM) hyperinsulinemic clamps were performed on normoglycemic insulin-sensitive (NIS) men and men who were normoglycemic but insulin resistant (NIR) and hyperglycemic and insulin resistant (HIR) (i.e., noninsulin-dependent diabetes mellitus). Insulin was infused at successive rates of 40 and 400 mU.m-2.min-1, and biopsies were obtained from the quadriceps femoris muscles before and after insulin and analyzed for regulators of phosphofructokinase, a rate-limiting enzyme for glycolysis. Glucose disposal and whole body carbohydrate oxidation were markedly lower in NIR and HIR vs. NIS (P < 0.001 for disposal and oxidation). The alpha-D-glucose 1,6-bisphosphate (G-1,6-P2) content increased almost twofold during the 40-mU insulin infusion (P < 0.001) without any further change during the 400-mU infusion in NIS men. The increase in G-1,6-P2 in NIR and HIR was only approximately 25 and 50% of the increase observed in NIS during the 40- and 400-mU infusions, respectively. The mean content of G-1,6-P2 was strongly related to the mean rate of carbohydrate oxidation (r = 0.99; P < 0.001). Because during euglycemic hyperinsulinemia approximately 90% of the glucose utilization is accounted for by skeletal muscle (J. Clin. Invest. 76: 149, 1985), it is likely that whole body carbohydrate oxidation is proportional to carbohydrate oxidation and glycolysis in muscle. The different rates of carbohydrate oxidation between NIS and insulin-resistant men could not be associated with differences in fructose 6-phosphate, fructose 1,6-bisphosphate, fructose 2,6-bisphosphate, P(i), free ADP and free AMP (activators of phosphofructokinase), or ATP and citrate (inhibitors of phosphofructokinase). It is suggested that under the present conditions, insulin-mediated glycolysis in human skeletal muscle is, at least partly, a result of phosphofructokinase activation by G-1,6-P2. C1 NIDDKD,CLIN DIABET & NUTR SECT,PHOENIX,AZ 85016. RP KATZ, A (reprint author), UNIV ILLINOIS,DEPT KINESIOL,906 S GODWIN AVE,URBANA,IL 61801, USA. NR 34 TC 11 Z9 11 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD JAN PY 1991 VL 260 IS 1 BP R113 EP R119 PN 2 PG 7 WC Physiology SC Physiology GA EU071 UT WOS:A1991EU07100055 PM 1825156 ER PT J AU WASTNEY, ME GOKMEN, IG AAMODT, RL RUMBLE, WF GORDON, GE HENKIN, RI AF WASTNEY, ME GOKMEN, IG AAMODT, RL RUMBLE, WF GORDON, GE HENKIN, RI TI KINETIC-ANALYSIS OF ZINC-METABOLISM IN HUMANS AFTER SIMULTANEOUS ADMINISTRATION OF ZN-65 AND ZN-70 SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE COMPARTMENTAL ANALYSIS; PHYSIOLOGICAL MODELS; TRACE METALS; STABLE ISOTOPES; RADIOACTIVE ISOTOPES ID NEUTRON-ACTIVATION ANALYSIS; STABLE ISOTOPE TRACER; YOUNG MEN; MASS-SPECTROMETRY; HUMAN-BLOOD; ABSORPTION; BIOAVAILABILITY; IRON; SAMPLES; PLASMA AB Zinc kinetics were studied and compared after oral simultaneous administration of two tracers, radioactive (Zn-65) and stable (Zn-70) isotope, to four normal human volunteers. Both tracers and zinc concentration were measured in plasma, red blood cells (RBC), urine, and feces for up to 78 days. Radioactive zinc was also measured by external counting over whole body, liver, and thigh. Data from each individual were analyzed using a compartmental model for zinc metabolism. Values calculated for absorption, fractional zinc excretion in urine, exchange with RBC, and secretion into gut using Zn-70 data did not differ from values calculated using Zn-65 data. Results show that human zinc metabolism can be investigated using stable isotopes as tracers to determine parameters of whole body zinc metabolism, including zinc absorption, excretion, and secretion. C1 TASTE & SMELL CLIN,WASHINGTON,DC 20016. MIDDLE E TECH UNIV,DEPT CHEM,ANKARA 06531,TURKEY. UNIV MARYLAND,DEPT CHEM,COLLEGE PK,MD 20742. NIH,DEPT NUCL MED,BETHESDA,MD 20892. RP WASTNEY, ME (reprint author), GEORGETOWN UNIV,MED CTR,HOSP 35,DEPT PEDIAT,3800 RESERVOIR RD NW,WASHINGTON,DC 20007, USA. FU NIA NIH HHS [AG-04511, AG-06840] NR 40 TC 27 Z9 28 U1 0 U2 2 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD JAN PY 1991 VL 260 IS 1 BP R134 EP R141 PN 2 PG 8 WC Physiology SC Physiology GA EU071 UT WOS:A1991EU07100058 PM 1992814 ER PT J AU LINET, MS CELENTANO, DD STEWART, WF AF LINET, MS CELENTANO, DD STEWART, WF TI HEADACHE CHARACTERISTICS ASSOCIATED WITH PHYSICIAN CONSULTATION - A POPULATION-BASED SURVEY SO AMERICAN JOURNAL OF PREVENTIVE MEDICINE LA English DT Article AB In a population-based telephone interview survey of 9,380 Washington County, Maryland, residents 12-29 years of age who reported a headache in the prior year, only 26.7% of women and 13.6% of men had ever sought a physician's advice for a headache problem. Women (13.9%) were more than twice as likely as men (5.6%) to have consulted a doctor for this condition within the previous 12 months. The likelihood of seeking medical care for headache increased with age among women but not men. Married women were more likely to have consulted with a physician for a headache problem than single or divorced women. Men and women consulting a doctor for this disorder within the previous 12 months described recent headaches (within the prior week) that were more severe, of longer duration (women only), and more likely to have migraine characteristics than recent headaches of persons not seeking medical attention. Compared with persons never consulting a physician for a headache problem, men and women who sought medical care had elevated attack rates of certain, probable, and possible migraine and mixed migraine-tension type headaches within the preceding week. Differences in attack rates for migraine and mixed migraine-tension headaches between those who recently and those who more than 12 months ago sought a physician's advice were more striking for men than for women. RP LINET, MS (reprint author), NCI,DIV CANC ETIOL,ANALYT STUDIES SECT,BIOSTAT BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 49 Z9 49 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 SN 0749-3797 J9 AM J PREV MED JI Am. J. Prev. Med. PD JAN-FEB PY 1991 VL 7 IS 1 BP 40 EP 46 PG 7 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA FP097 UT WOS:A1991FP09700008 PM 1867898 ER PT J AU SCHNEIDER, ML MOORE, CF SUOMI, SJ CHAMPOUX, M AF SCHNEIDER, ML MOORE, CF SUOMI, SJ CHAMPOUX, M TI LABORATORY ASSESSMENT OF TEMPERAMENT AND ENVIRONMENTAL ENRICHMENT IN RHESUS-MONKEY INFANTS (MACACA-MULATTA) SO AMERICAN JOURNAL OF PRIMATOLOGY LA English DT Article DE ENRICHMENT; RHESUS MONKEY; NEONATAL BEHAVIOR; MACACA-MULATTA ID PERSONALITY; BEHAVIOR; BRAIN; SEX AB This study investigated the combined effects of early temperamental characteristics and environmental enrichment on a variety of developmental measures in nursery-reared rhesus monkey infants. Twenty-three infants, reared in either standard laboratory cages or enriched environments, were tested during the lst month of life for interactive, motor, and temperamental capabilities and characteristics. At 8 months of age, all subjects were assessed on a second series of tests designed to measure their problem-solving skills, motor capabilities, and temperamental responses under challenge. Results indicated that enrichment was associated with higher scores on subsequent problem-solving and motor tests. However, such effects were found to combine with early temperament ratings. Specifically, individuals performing best on the 8-month tests had not only been reared in enriched environments, but also had been rated low on fearfulness during the early assessment. In addition, individuals scoring poorest had been rated as fearful initially in addition to being reared without enrichment. Results indicated that while high ratings on early laboratory assessments of fearfulness may be predictive of poorer problem-solving performance under challenging conditions, these adverse effects may be partially attenuated by environmental enrichment. C1 UNIV WISCONSIN,MADISON,WI 53706. NICHHD,COMPARAT ETHOL LAB,BETHESDA,MD 20892. NR 49 TC 51 Z9 52 U1 1 U2 5 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0275-2565 J9 AM J PRIMATOL JI Am. J. Primatol. PY 1991 VL 25 IS 3 BP 137 EP 155 DI 10.1002/ajp.1350250302 PG 19 WC Zoology SC Zoology GA GN672 UT WOS:A1991GN67200001 ER PT J AU JOHNSON, EO KAMILARIS, TC CARTER, S GOLD, PW CHROUSOS, GP AF JOHNSON, EO KAMILARIS, TC CARTER, S GOLD, PW CHROUSOS, GP TI ENVIRONMENTAL-STRESS AND REPRODUCTIVE SUCCESS IN THE COMMON MARMOSET (CALLITHRIX-JACCHUS-JACCHUS) SO AMERICAN JOURNAL OF PRIMATOLOGY LA English DT Note DE ENVIRONMENTAL STABILITY; REPRODUCTIVE STATUS; PARITY ID CORTICOTROPIN-RELEASING FACTOR; BABOONS PAPIO-ANUBIS; MALE RATS; ENDOCRINE; GONADOTROPIN; TESTOSTERONE; SUPPRESSION; MONKEYS; LH AB The present report documents the breeding success of a new colony of common marmosets (Callithrix jacchus jacchus) and demonstrates a correlation between environmental stress and reproductive success. Environmental conditions ranged chronologically over 40 months, through four periods: I) the initial period, when the colony was formed; II) a phase of relative environmental stability; III) a stage of "environmental stress" (when the colony was disrupted by nearby construction); and IV) a return to a stable environment. Examination of reproductive status during each period indicated that the colony exhibited severe reproductive suppression during the time of the environmental disruption. Parity and the number of live births decreased and the number of spontaneous abortions increased during this period. Reproductive success remained low during period IV. More triplets than twins were born during the period of relative environmental stability. C1 NIMH,CLIN NEUROENDOCRINOL BRANCH,BETHESDA,MD 20892. UNIV MARYLAND,DEPT ZOOL,COLLEGE PK,MD 20742. RP JOHNSON, EO (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,BLDG 10,RM 10N262,BETHESDA,MD 20892, USA. NR 49 TC 23 Z9 23 U1 1 U2 5 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0275-2565 J9 AM J PRIMATOL JI Am. J. Primatol. PY 1991 VL 25 IS 3 BP 191 EP 201 DI 10.1002/ajp.1350250306 PG 11 WC Zoology SC Zoology GA GN672 UT WOS:A1991GN67200005 ER PT J AU BAYNE, K MAINZER, H DEXTER, S CAMPBELL, G YAMADA, F SUOMI, S AF BAYNE, K MAINZER, H DEXTER, S CAMPBELL, G YAMADA, F SUOMI, S TI THE REDUCTION OF ABNORMAL BEHAVIORS IN INDIVIDUALLY HOUSED RHESUS-MONKEYS (MACACA-MULATTA) WITH A FORAGING GROOMING BOARD SO AMERICAN JOURNAL OF PRIMATOLOGY LA English DT Article DE ENVIRONMENTAL ENRICHMENT; FLEECE; ABERRANT BEHAVIOR ID ENRICHMENT; MACAQUES AB A new enviromental enrichment device, termed a "foraging/grooming board," was presented to 8 individually housed rhesus monkeys for the explicit purpose of reducing the level of aberrant behaviors manifested by these animals. The device, consisting of a piece of plexiglass covered with artificial fleece, had particles of food treats rubbed into it and was attached to the outside of each animal's home cage. All animals foraged from the board to the point that a significant reduction in the level of abnormal behavior was noted. Most animals also groomed the fleece covering the board, utilizing the same motor patterns that would be directed toward grooming another monkey. These boards are inexpensive to construct and easy to sanitize, and do not require placing animal facility personnel at risk to maintain them. C1 NICHHD,DIV COMP RES & TECHNOL,STAT & MATH METHODOL LAB,BETHESDA,MD 20892. NICHHD,COMPARAT ETHOL LAB,BETHESDA,MD 20892. RP BAYNE, K (reprint author), NICHHD,OFF DIRECTOR,OFF ANIM CARE & USE,BLDG 14D,RM 313,BETHESDA,MD 20892, USA. NR 42 TC 69 Z9 69 U1 1 U2 19 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0275-2565 J9 AM J PRIMATOL JI Am. J. Primatol. PY 1991 VL 23 IS 1 BP 23 EP 35 DI 10.1002/ajp.1350230104 PG 13 WC Zoology SC Zoology GA EP474 UT WOS:A1991EP47400003 ER PT J AU BYRNE, GD SUOMI, SJ AF BYRNE, GD SUOMI, SJ TI EFFECTS OF WOODCHIPS AND BURIED FOOD ON BEHAVIOR PATTERNS AND PSYCHOLOGICAL WELL-BEING OF CAPTIVE RHESUS-MONKEYS SO AMERICAN JOURNAL OF PRIMATOLOGY LA English DT Article DE RHESUS; ENRICHMENT; BEHAVIOR; FORAGING; CORTISOL ID ENRICHMENT AB The effects of adding woodchip litter to bare-floored pens, burying monkey chow in the woodchips, and scattering sunflower seeds in woodchips was studied in 2 stable social groups of rhesus macaques (Macaca mulatta) to ascertain the effects of these manipulations on levels of foraging, exploration, abnormal behavior, social interactions, and urinary cortisol levels. The addition of woodchips increased exploration and feeding levels and decreased social interactions. Burial of regular monkey chow in woodchips had little effect on behavior beyond that of the woodchips alone, increasing exploration and decreasing passivity. The addition of sunflower seeds to the woodchips encouraged increased feeding and exploration and led to decreases in passivity and social interaction. There was little discernible effect of woodchip enrichment on urinary cortisol values. In contrast to some previous studies, there was no effect of wood chips or sunflower seeds on the occurrence of agonistic interactions, play, or abnormal behavior patterns. C1 NICHHD,COMPARAT ETHOL LAB,BETHESDA,MD 20892. NR 32 TC 42 Z9 43 U1 2 U2 12 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0275-2565 J9 AM J PRIMATOL JI Am. J. Primatol. PY 1991 VL 23 IS 3 BP 141 EP 151 DI 10.1002/ajp.1350230302 PG 11 WC Zoology SC Zoology GA FB430 UT WOS:A1991FB43000001 ER PT J AU GOLDBERG, TE BIGELOW, LB WEINBERGER, DR DANIEL, DG KLEINMAN, JE AF GOLDBERG, TE BIGELOW, LB WEINBERGER, DR DANIEL, DG KLEINMAN, JE TI COGNITIVE AND BEHAVIORAL-EFFECTS OF THE COADMINISTRATION OF DEXTROAMPHETAMINE AND HALOPERIDOL IN SCHIZOPHRENIA SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID DORSOLATERAL PREFRONTAL CORTEX; LOW-DOSE BROMOCRIPTINE; D2 DOPAMINE-RECEPTORS; PHYSIOLOGIC DYSFUNCTION; NUCLEUS ACCUMBENS; D-AMPHETAMINE; BRAIN; DRUGS; NEUROLEPTICS; APOMORPHINE AB Objective: The authors sought to determine if an acute dose of dextroamphetamine might have positive effects on affect and cognition in schizophrenic patients maintained on a regimen of haloperidol and, if so, what variables might predict such improvements. Method: Twenty-one patients with chronic schizophrenia who were hospitalized on a research ward received a single oral dose of dextroamphetamine (0.25 mg/kg) in a double-blind, placebo-controlled, crossover study. All patients were receiving 0.4 mg/kg per day of haloperidol. Cognitive tests, motor tests, global ratings, mood ratings and videotape ratings were used to determine the effect of the coadministration of these drugs. Ventricle-brain ratios derived from CT scans were used to predict response to the coadministration of these drugs. Results: Amphetamine improved performance on a measure of concept formation on the Wisconsin Card Sorting Test but did not result in changes in performance on tests of memory or attention. As a group, the patients were more active and performed psychomotor tests more quickly while receiving amphetamine. Six patients were judged by clinical raters to have improved in terms of affect, cooperation, and engagement with the environment. Improvement was associated with enlarged cerebral ventricles and increases in blink rate from the placebo to the active drug condition. No patient unequivocally worsened. Conclusions: These results may be consistent with the theory that coadministration of amphetamine and haloperidol produces relatively selective enhancement of cortical dopaminergic activity. However, because of the acute nature of the trial and the specialized research environment in which it was conducted, the authors do not advocate amphetamine as a routine clinical treatment of schizophrenia. RP GOLDBERG, TE (reprint author), NIMH,NEUROSCI CTR ST ELIZABETHS,INTRAMURAL RES PROGRAM,WASHINGTON,DC 20032, USA. NR 56 TC 87 Z9 88 U1 0 U2 1 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD JAN PY 1991 VL 148 IS 1 BP 78 EP 84 PG 7 WC Psychiatry SC Psychiatry GA EQ124 UT WOS:A1991EQ12400013 PM 1984711 ER PT J AU OLEARY, KM BROUWERS, P GARDNER, DL COWDRY, RW AF OLEARY, KM BROUWERS, P GARDNER, DL COWDRY, RW TI NEUROPSYCHOLOGICAL TESTING OF PATIENTS WITH BORDERLINE PERSONALITY-DISORDER SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID INTERVIEW AB Objective and Method: This study examined whether a battery of neuropsychological tests could detect cognitive deficits - particularly in the areas of perception, learning, and memory - in patients with borderline personality disorder. The test battery was completed by 16 research outpatients with borderline personality disorder, typified by behavioral dyscontrol and diagnosed according to DSM-III-R criteria and the Diagnostic Interview for Borderline Patients. A comparison group of 16 normal volunteers also completed the test battery. Results: The performance of the borderline patients was significantly impaired in comparison with that of the normal group on memory test requiring uncued recall of complex, recently learned material. Cues given on an auditory memory task partially corrected that deficit. The patients' performance was also significantly impaired on several visual perceptual tests. These deficits do not appear to have been attributable to attentional problems, psychomotor impairment, current major depression, or history of alcohol abuse. Conclusion: The observed difficulties in separating essential from extraneous visual information and in recalling complex material may be relevant in understanding some of the clinical features of borderline personality disorder. The observed memory improvement resulting from cueing suggests specific strategies that may be used to aid patients' recall of complex material. RP OLEARY, KM (reprint author), NIMH,NEUROSCI CTR ST ELIZABETHS,DIV INTRAMURAL RES PROGRAMS,WASHINGTON,DC 20032, USA. NR 23 TC 81 Z9 82 U1 1 U2 2 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD JAN PY 1991 VL 148 IS 1 BP 106 EP 111 PG 6 WC Psychiatry SC Psychiatry GA EQ124 UT WOS:A1991EQ12400018 PM 1984693 ER PT J AU PATO, MT PIGOTT, TA HILL, JL GROVER, GN BERNSTEIN, S MURPHY, DL AF PATO, MT PIGOTT, TA HILL, JL GROVER, GN BERNSTEIN, S MURPHY, DL TI CONTROLLED COMPARISON OF BUSPIRONE AND CLOMIPRAMINE IN OBSESSIVE-COMPULSIVE DISORDER SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID DOUBLE-BLIND; TRIAL AB Eighteen outpatients with obsessive-compulsive disorder were treated with either buspirone, a partial serotonin agonist, or clomipramine, a serotonin uptake inhibitor, in a double-blind, random-assignment study. Both drugs led to statistically significant and similar improvements in scores on the Yale-Brown Obsessive-Compulsive Rating Scale and other obsessive-compulsive and depression scales. This preliminary result warrants further exploration with a large sample and other serotonergic agents. C1 NIH,CLIN SCI LAB,BETHESDA,MD 20892. NIH,CTR CLIN,DEPT NURSING,BETHESDA,MD 20892. FU NICHD NIH HHS [HD-06930] NR 10 TC 88 Z9 90 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD JAN PY 1991 VL 148 IS 1 BP 127 EP 129 PG 3 WC Psychiatry SC Psychiatry GA EQ124 UT WOS:A1991EQ12400022 PM 1984696 ER PT J AU DOPPMAN, JL PASS, HI NIEMAN, LK FINDLING, JW DWYER, AJ FEUERSTEIN, IM LING, A TRAVIS, WD CUTLER, GB CHROUSOS, GP LORIAUX, DL AF DOPPMAN, JL PASS, HI NIEMAN, LK FINDLING, JW DWYER, AJ FEUERSTEIN, IM LING, A TRAVIS, WD CUTLER, GB CHROUSOS, GP LORIAUX, DL TI DETECTION OF ACTH-PRODUCING BRONCHIAL CARCINOID-TUMORS - MR IMAGING VS CT SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article ID WHOLE LUNG TOMOGRAPHY; COMPUTED-TOMOGRAPHY; PULMONARY NODULES; BRONCHOGENIC-CARCINOMA; METASTASES AB Adrenocorticotrophic hormone (ACTH)-producing bronchial carcinoid tumors tend to occur in the middle third of the lung adjacent to pulmonary vessels. Because they cause signs and symptoms when quite small (by virtue of their ACTH production), they may not be detected by CT. MR imaging was performed in 10 consecutive patients with surgically proved ACTH-producing bronchial carcinoid tumors in order to test the ability of MR to clarify equivocal or indeterminate findings on CT examinations. All bronchial carcinoid tumors had high signal intensity on T2-weighted and short-inversion-time inversion-recovery images, facilitating their distinction from pulmonary vasculature. In eight patients, the CT and MR images were equivalent in the detection of bronchial carcinoid tumors. In two patients, MR showed tumors in the middle third of the lung that were equivocal on CT. MR imaging may distinguish small bronchial carcinoid tumors from adjacent pulmonary vessels in the central third of the lung at a time when the CT study is nondiagnostic or equivocal. C1 ST LUKES HOSP,DEPT MED,MILWAUKEE,WI 53215. NCI,DIV CANC TREATMENT,SURG BRANCH,BETHESDA,MD 20892. GEORGETOWN UNIV,MED CTR,DEPT RADIOL,WASHINGTON,DC 20007. NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. NCI,DIV CANC BIOL & DIAG,PATHOL LAB,BETHESDA,MD 20892. RP DOPPMAN, JL (reprint author), NIH,WARREN G MAGNUSON CLIN CTR,DEPT DIAGNOST RADIOL,BLDG 10,ROOM 1C660,BETHESDA,MD 20892, USA. NR 29 TC 60 Z9 60 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD JAN PY 1991 VL 156 IS 1 BP 39 EP 43 PG 5 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EP262 UT WOS:A1991EP26200006 PM 1845787 ER PT J AU ROSENBERG, JM WHITAKER, JH AF ROSENBERG, JM WHITAKER, JH TI BILATERAL INFRAPATELLAR TENDON-RUPTURE IN A PATIENT WITH JUMPERS KNEE SO AMERICAN JOURNAL OF SPORTS MEDICINE LA English DT Article RP ROSENBERG, JM (reprint author), NIA,NEUROSCI LAB,BLDG 10,RM 6C103,9000 ROCKVILLE PK,BETHESDA,MD 20892, USA. NR 0 TC 20 Z9 23 U1 0 U2 1 PU AMER ORTHOPAEDIC SOC SPORT MED PI WALTHAM PA 230 CALVARY STREET, WALTHAM, MA 02154 SN 0363-5465 J9 AM J SPORT MED JI Am. J. Sports Med. PD JAN-FEB PY 1991 VL 19 IS 1 BP 94 EP 95 DI 10.1177/036354659101900117 PG 2 WC Orthopedics; Sport Sciences SC Orthopedics; Sport Sciences GA EV643 UT WOS:A1991EV64300017 PM 2008939 ER PT J AU LACK, EE SCHLOO, BL AZUMI, N TRAVIS, WD GRIER, HE KOZAKEWICH, HPW AF LACK, EE SCHLOO, BL AZUMI, N TRAVIS, WD GRIER, HE KOZAKEWICH, HPW TI UNDIFFERENTIATED (EMBRYONAL) SARCOMA OF THE LIVER - CLINICAL AND PATHOLOGICAL-STUDY OF 16 CASES WITH EMPHASIS ON IMMUNOHISTOCHEMICAL FEATURES SO AMERICAN JOURNAL OF SURGICAL PATHOLOGY LA English DT Article DE UNDIFFERENTIATED SARCOMA; EMBRYONAL SARCOMA; LIVER ID MALIGNANT FIBROUS HISTIOCYTOMA; HEPATOCELLULAR-CARCINOMA; CYTOKERATIN EXPRESSION; SMOOTH-MUSCLE; TUMORS; CHILDHOOD; RHABDOMYOSARCOMA; MESENCHYMOMA; KERATIN; CHILDREN AB Undifferentiated (embryonal) sarcoma of the liver is a primitive mesenchymal neoplasm with predilection for individuals in the first 2 decades of life. In this study (10 boys, 6 girls), children in the age range of 6-10 years were most commonly affected (63%). Clinical features most frequently noted on presentation were abdominal pain or a palpable mass. In two cases there was cardiac involvement caused by invasion of the inferior vena cava with extension into the right atrium and ventricle; both children died of progressive dyspnea from tumor embolization to the lungs. One patient was a member of a kindred with the cancer family syndrome (Li-Fraumeni syndrome). There were 13 tumor-related deaths (86% mortality); one child was alive with recurrent tumor in the upper abdomen. Complete surgical resection was attempted in 10 of 15 children who underwent exploratory laparotomy; 2 were alive and well 1 and 5 years later, whereas 1 patient had a recurrence in the upper abdomen 3 years after diagnosis. Ultrastructural study (five cases) and immunohistochemistry (11 cases) supported a mesenchymal origin for the tumor, but failed to identify any diagnostic immunophenotype or specific line of differentiation. Coexpression of vimentin and cytokeratin was seen in three cases. Prompt detection of this aggressive tumor with complete surgical resection is the key to a successful outcome, but this is very difficult to achieve. Recent experience suggests that aggressive adjuvant chemotherapy may improve survival in some cases. C1 CHILDRENS HOSP MED CTR,DEPT PATHOL,BOSTON,MA 02115. NIH,DEPT PATHOL,BETHESDA,MD 20892. HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV PEDIAT HEMATOL ONCOL,BOSTON,MA 02115. RP LACK, EE (reprint author), GEORGETOWN UNIV,SCH MED,DEPT PATHOL,BASIC SCI BLDG,ROOM 161,3900 RESERVOIR RD NW,WASHINGTON,DC 20007, USA. NR 50 TC 66 Z9 73 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0147-5185 J9 AM J SURG PATHOL JI Am. J. Surg. Pathol. PD JAN PY 1991 VL 15 IS 1 BP 1 EP 16 DI 10.1097/00000478-199101000-00001 PG 16 WC Pathology; Surgery SC Pathology; Surgery GA ER243 UT WOS:A1991ER24300001 PM 1702267 ER PT J AU GONZALEZ, CL MEDEIROS, LJ BRAZIEL, RM JAFFE, ES AF GONZALEZ, CL MEDEIROS, LJ BRAZIEL, RM JAFFE, ES TI T-CELL LYMPHOMA INVOLVING SUBCUTANEOUS TISSUE - A CLINICOPATHOLOGICAL ENTITY COMMONLY ASSOCIATED WITH HEMOPHAGOCYTIC SYNDROME SO AMERICAN JOURNAL OF SURGICAL PATHOLOGY LA English DT Article DE T-CELL LYMPHOMA; HEMOPHAGOCYTOSIS; SUBCUTANEOUS TISSUE; CUTANEOUS LYMPHOMA; PANNICULITIS ID ANGIOCENTRIC IMMUNOPROLIFERATIVE LESIONS; HISTIOCYTIC MEDULLARY RETICULOSIS; MALIGNANT HISTIOCYTOSIS; LYMPHOCYTIC-LEUKEMIA; HODGKINS-DISEASE; ERYTHROPHAGOCYTOSIS; PROLIFERATIONS; PANNICULITIS; PHAGOCYTOSIS; HYPERPLASIA AB Eight cases of T-cell lymphoma localized primarily to the subcutaneous adipose tissue are described, five of which were referred in consultation with a benign diagnosis having been made or suggested. All patients presented with 1-12-cm-diameter subcutaneous nodules, which preferentially involved the extremities in six individuals. Histologically, the lesions were reminiscent of panniculitis and were composed of a mixture of small and large atypical lymphoid cells (large cells predominated in four cases) infiltrating between adipocytes. Focally, sheets of tumor cells were found. Karyorrhexis, fat necrosis, and benign histiocytes were present in all cases. Involvement of small blood vessels was found in seven cases, but the infiltrates were not primarily angiocentric, and angiodestruction was minimal or absent. Immunophenotypic analysis (paraffin or frozen sections) in all cases showed that the atypical cells were of T-cell phenotype. Frozen-section studies demonstrated a mature T-cell phenotype with evidence of pan-T-cell antigen loss in two of five lesions. Genotypic analysis demonstrated a rearrangement of the T-cell receptor beta-chain gene in one (possibly two) biopsies of three cases studied. All patients had some evidence of hemophagocytosis during their clinical course. Six patients developed a florid hemophagocytic syndrome, fatal in five patients. Autopsies were done in all of the expired patients, and all had residual subcutaneous lymphoma and a hemophagocytic syndrome. Dissemination to nonsubcutaneous sites did not occur. Three patients are currently alive without evidence of lymphoma after aggressive chemotherapy (mean follow-up, 12 months). These results suggest that T-cell lymphomas that are primarily localized to the subcutaneous tissue may represent a distinct clinicopathologic entity. Initial biopsy findings may be misinterpreted as benign. A hemophagocytic syndrome commonly supervenes that may be secondary to lymphokine production by the malignant cells or related to the destruction of normal cells at subcutaneous sites. C1 NCI,HEMATOPATHOL SECT,PATHOL LAB,BLDG 10,ROOM 2N202,BETHESDA,MD 20892. OREGON HLTH SCI UNIV,PORTLAND,OR 97201. NR 34 TC 275 Z9 304 U1 0 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0147-5185 J9 AM J SURG PATHOL JI Am. J. Surg. Pathol. PD JAN PY 1991 VL 15 IS 1 BP 17 EP 27 DI 10.1097/00000478-199101000-00002 PG 11 WC Pathology; Surgery SC Pathology; Surgery GA ER243 UT WOS:A1991ER24300002 PM 1985499 ER PT J AU WILLIAMS, J SCHNED, A COTELINGAM, JD JAFFE, ES AF WILLIAMS, J SCHNED, A COTELINGAM, JD JAFFE, ES TI CHRONIC LYMPHOCYTIC-LEUKEMIA WITH COEXISTENT HODGKINS-DISEASE - IMPLICATIONS FOR THE ORIGIN OF THE REED-STERNBERG CELL SO AMERICAN JOURNAL OF SURGICAL PATHOLOGY LA English DT Article DE HODGKINS DISEASE; REED-STERNBERG CELLS; RICHTERS SYNDROME; MALIGNANT LYMPHOMA ID RICHTERS SYNDROME; ANTIBODIES; EXPRESSION; LYMPHOMA; IMMUNOGLOBULIN; FEATURES; ANTIGENS; MARKER; TISSUE AB The association of chronic lymphocytic leukemia (CLL) and Hodgkin's disease has been controversial. Pleomorphic lymphoreticular cells resembling Reed-Sternberg cells have been observed in Richter's syndrome. Although most observers have favored the view that these cells are a component of a pleomorphic non-Hodgkin's lymphoma, some cases of histologically typical Hodgkin's disease have been described. We have studied two cases that appear to represent composite CLL and Hodgkin's disease, providing evidence for an interrelationship of these two disorders. Classic Reed-Sternberg cells and variants (RS-H) were seen in a background that was otherwise typical of CLL. Both patients initially presented with characteristic findings of CLL in the peripheral blood and bone marrow. The first patient was found to have RS-H cells within lymph nodes at initial presentation, and ultimately progressed to develop a disseminated lymphoma characteristic of Hodgkin's disease. In the second patient, RS-H cells were not discovered until 5 years later. Immunophenotypic studies confirmed these morphologic impressions. The predominant lymphocyte population had a phenotype consistent with B-cell CLL. By contrast, the RS-H cells were strongly positive for CD15 (Leu M1) with staining of the Golgi region and cell membrane. Additionally, the RS-H cells were surrounded by rosettes of lymphocytes that marked as T cells. In both of the patients, a small percentage of RS-H cells expressed positivity for the B-cell marker L-26, which may indicate an origin from the underlying CLL. These findings support a B-cell origin for the malignant cell in some cases of Hodgkin's disease and suggest that Hodgkin's disease in some patients may be related to or derived from a coexisting lymphoid malignancy. C1 NCI, HEMATOPATHOL SECT, PATHOL LAB, BLDG 10, ROOM 2N202, BETHESDA, MD 20892 USA. NATL NAVAL MED CTR, DEPT PATHOL, BETHESDA, MD 20814 USA. NATL NAVAL MED CTR, HEMATOPATHOL BRANCH, BETHESDA, MD 20814 USA. VET ADM MED CTR, DEPT PATHOL, WHITE RIVER JCT, VT USA. NR 36 TC 73 Z9 74 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0147-5185 J9 AM J SURG PATHOL JI Am. J. Surg. Pathol. PD JAN PY 1991 VL 15 IS 1 BP 33 EP 42 DI 10.1097/00000478-199101000-00004 PG 10 WC Pathology; Surgery SC Pathology; Surgery GA ER243 UT WOS:A1991ER24300004 PM 1670753 ER PT J AU VIEIRA, LQ CORREAOLIVEIRA, R KATZ, N DESOUZA, CP CARVALHO, OS ARAUJO, N SHER, A BRINDLEY, PJ AF VIEIRA, LQ CORREAOLIVEIRA, R KATZ, N DESOUZA, CP CARVALHO, OS ARAUJO, N SHER, A BRINDLEY, PJ TI GENOMIC VARIABILITY IN FIELD POPULATIONS OF SCHISTOSOMA-MANSONI IN BRAZIL AS DETECTED WITH A RIBOSOMAL GENE PROBE SO AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE LA English DT Article ID DNA; RESISTANCE; DIFFERENTIATION; HYCANTHONE AB A cloned fragment of the ribosomal gene of Schistosoma mansoni, pSM 389, which contains part of the small rRNA gene plus a portion of the nontranscribed intergenic spacer, was used in Southern hybridization analyses to investigate genomic variation in natural populations of S. mansoni in Brazil. Genomic DNAs were isolated from schistosomes from infected patients (some of whom did not respond to antischistosomal chemotherapy), and from snails from disparate geographic locations in Brazil. Restriction fragment length polymorphisms (RFLPs) were evident in Southern blot hybridizations of these schistosome DNAs, and the RFLPs indicated that the genomic profiles of a number of Brazilian strains were more similar to each other than they were to parasites from two laboratory reference strains of Puerto Rican origin. In addition, the Brazilian isolates could generally be separated from each other based on these RFLPs. Isolates from the southeastern state of Minas Gerais were more similar to each other than they were to parasites isolated in the northeastern states of Alagoas and Pernambuco. Variation was evident among individual worms from some of the isolates, and these individual variations contributed to the complex RFLP patterns that were characteristic for particular isolates. The variation within a natural population isolated directly from snails at Ressaca, Belo Horizonte, may be more marked than that exhibited by more established strains maintained in the laboratory for numerous generations. C1 NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. UNIV FED MINAS GERAIS,DEPT BIOQUIM & IMUNOL,BR-30000 BELO HORIZONTE,MG,BRAZIL. RP VIEIRA, LQ (reprint author), FDN OSWALDO CRUZ,CTR PESQUISAS RENE RACHOU,BELO HORIZONTE,MG,BRAZIL. RI Vieira, Leda/G-7487-2014 NR 32 TC 35 Z9 35 U1 0 U2 0 PU AMER SOC TROP MED & HYGIENE PI MCLEAN PA 8000 WESTPARK DRIVE SUITE 130, MCLEAN, VA 22101 SN 0002-9637 J9 AM J TROP MED HYG JI Am. J. Trop. Med. Hyg. PD JAN PY 1991 VL 44 IS 1 BP 69 EP 78 PG 10 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA EY659 UT WOS:A1991EY65900010 PM 1671740 ER PT B AU DALAKAS, M ILLA, I AF DALAKAS, M ILLA, I BE ROWLAND, LP TI POSTPOLIO SYNDROME - CONCEPTS IN CLINICAL-DIAGNOSIS, PATHOGENESIS, AND ETIOLOGY SO AMYOTROPHIC LATERAL SCLEROSIS AND OTHER MOTOR NEURON DISEASES SE ADVANCES IN NEUROLOGY LA English DT Proceedings Paper CT MEETING ON AMYOTROPHIC LATERAL SCLEROSIS AND OTHER MOTOR NEURON DISEASES CY DEC, 1989 CL TUCSON, AZ SP MUSCULAR DYSTROPHY ASSOC RP DALAKAS, M (reprint author), NINCDS,MED NEUROL BRANCH,NEUROMUSCULAR DIS SECT,9000 ROCKVILLE PIKE,BLDG 10,ROOM 4N-248,BETHESDA,MD 20892, USA. NR 0 TC 53 Z9 55 U1 0 U2 1 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-748-5 J9 ADV NEUROL JI Adv.Neurol. PY 1991 VL 56 BP 495 EP 511 PG 17 WC Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA BT38Q UT WOS:A1991BT38Q00044 PM 1853779 ER PT J AU TAN, TH AF TAN, TH TI A NONRADIOACTIVE SCREENING METHOD FOR CLONING GENES ENCODING SEQUENCE-SPECIFIC DNA-BINDING PROTEINS SO ANALYTICAL BIOCHEMISTRY LA English DT Article ID MOLECULAR-CLONING; EXPRESSION; BACTERIOPHAGE; ENHANCER; PROBES RP TAN, TH (reprint author), NCI,FREDERICK CANC RES & DEV CTR,BIOL CARCINOGENESIS & DEV PROGRAM,PRI DYNCORP,POB B,FREDERICK,MD 21702, USA. RI Tan, Tse-Hua/E-3983-2010 OI Tan, Tse-Hua/0000-0003-4969-3170 FU NCI NIH HHS [N01 CO-74102] NR 9 TC 2 Z9 3 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-2697 J9 ANAL BIOCHEM JI Anal. Biochem. PD JAN PY 1991 VL 192 IS 1 BP 17 EP 22 DI 10.1016/0003-2697(91)90176-T PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA EP711 UT WOS:A1991EP71100004 PM 1828652 ER PT J AU MIXSON, AJ GRANGER, AN PHANG, JM AF MIXSON, AJ GRANGER, AN PHANG, JM TI AN ASSAY FOR PYRROLINE 5-CARBOXYLATE BASED ON ITS INTERACTION WITH CYSTEINE SO ANALYTICAL LETTERS LA English DT Article DE PYRROLINE 5-CARBOXYLATE; CYSTEINE-PYRROLINE 5-CARBOXYLATE ADDUCT; CATION EXCHANGE CHROMATOGRAPHY ID PURINE NUCLEOTIDE PRODUCTION; PYRROLINE-5-CARBOXYLIC ACID; HUMAN-ERYTHROCYTES; STIMULATION; CELLS AB Pyrroline 5-carboxylate, a potent effector of redox dependent pathways, is a nutritionally responsive plasma constituent and is present in tissue culture medium conditioned by a variety of cells. Studies of P5C production and release both in vivo and in vitro have become increasingly important. To date, the assay used is a specific, sensitive radioenzymatic method. Unfortunately, the necessary materials (purified P5C reductase and NADP[H-3], labeled in the transferable position) are not readily available. We now describe a method using commercially available reactants. This new method, based on the interaction of P5C with cysteine, is sensitive and specific; the presence of a wide variety of compounds only minimally affected the assay. [S-35] Cysteine at a defined specific activity is reacted with P5C and the P5C-CYS adduct can be separated from the reactants by cation exchange column chromatography. To optimize the assay conditions, we characterized the time course, pH dependence and cysteine concentrations for adduct formation. Studies showed that the sulfhydryl group of cysteine is critical in the formation of the P5C-CYS adduct. Comparison of this new method to the previously published radioenzymatic method showed close correlation over a wide range of P5C concentrations in conditioned media. C1 NCI,METAB BRANCH,ENDOCRINOL SECT,BETHESDA,MD 20892. NR 17 TC 2 Z9 2 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0003-2719 J9 ANAL LETT JI Anal. Lett. PY 1991 VL 24 IS 4 BP 625 EP 641 PG 17 WC Chemistry, Analytical SC Chemistry GA FR387 UT WOS:A1991FR38700007 ER PT J AU RUDNICK, S STEVENSON, GW HALL, SC ESPINOZADELGADO, I STEVENSON, HC LONGO, DL AF RUDNICK, S STEVENSON, GW HALL, SC ESPINOZADELGADO, I STEVENSON, HC LONGO, DL TI HALOTHANE POTENTIATES THE ANTITUMOR-ACTIVITY OF GAMMA-INTERFERON AND MIMICS CALMODULIN-BLOCKING AGENTS SO ANESTHESIOLOGY LA English DT Article DE ANESTHETICS, VOLATILE, HALOTHANE; CALMODULIN INHIBITORS, TRIFLUOPERAZINE (TFP); GAMMA-INTERFERON; TUMOR CELL CYTOTOXICITY ID CLINICAL IMPLICATIONS; LOCAL-ANESTHETICS; IMMUNE; ACTIVATION; IMMUNOLOGY; INHIBITION; CULTURES; SURGERY; CALCIUM AB This study reports effects of halothane on tumor cells in vitro. Cells from the human colon cancer cell line HT-29 were exposed to various concentrations of halothane for 8-72 h. The effect of this exposure on this colon cancer cell line, with and without coincubation with the biologic response modifier gamma-interferon (IFN-gamma), was studied. Using the tumor target cell survival (TTCS) assay, concentrations of halothane from 0.5 to 2% markedly augmented the antitumor activities of IFN-gamma against HT-29. The tumor cell cytostatic effects of IFN-gamma in the 0.75-6-unit/ml range were increased nearly 400% by concentrations of halothane as low as 1%. These results were confirmed in a separate cytolytic assay (Indium-111 release assay), which revealed that halothane concentrations in the 2-4% range markedly increased the cytolytic capacity of IFN-gamma at doses of IFN-gamma between 75 and 1,250 units/ml. The cytolytic activity of IFN-gamma was increased nearly 300% by doses of halothane as low as 1%. A nearly identical pattern of augmentation of IFN-gamma-induced antitumor activity was observed when the known calmodulin inhibitor trifluoperazine (TFP) was coincubated with IFN-gamma. At concentrations of 4-10-mu-M, the antitumor activity of IFN-gamma was increased nearly 400%. These observations suggest that the pattern of halothane potentiation of the antitumor activity of IFN-gamma is similar to that exhibited by known calmodulin inhibitors. C1 NORTHWESTERN UNIV,CHILDRENS MEM HOSP,SCH MED,DEPT PEDIAT ANESTHESIA,2300 CHILDRENS PLAZA,CHICAGO,IL 60614. NCI,FREDERICK CANC RES FACIL,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701. NR 26 TC 13 Z9 13 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0003-3022 J9 ANESTHESIOLOGY JI Anesthesiology PD JAN PY 1991 VL 74 IS 1 BP 115 EP 119 DI 10.1097/00000542-199101000-00019 PG 5 WC Anesthesiology SC Anesthesiology GA ET504 UT WOS:A1991ET50400019 PM 1898840 ER PT J AU BOINSKI, S AF BOINSKI, S TI THE COORDINATION OF SPATIAL POSITION - A FIELD-STUDY OF THE VOCAL BEHAVIOR OF ADULT FEMALE SQUIRREL-MONKEYS SO ANIMAL BEHAVIOUR LA English DT Article ID SAIMIRI-OERSTEDI; ISOLATION PEEPS; COSTA-RICA; VOCALIZATIONS; PREDATION; SCIUREUS; PATTERNS; HABITAT; FOREST; CALLS RP BOINSKI, S (reprint author), NICHHD,CTR ANIM,COMPARAT ETHOL LAB,POB 289,BLDG 112,POOLESVILLE,MD 20837, USA. NR 39 TC 84 Z9 85 U1 1 U2 14 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0003-3472 J9 ANIM BEHAV JI Anim. Behav. PD JAN PY 1991 VL 41 BP 89 EP 102 DI 10.1016/S0003-3472(05)80505-6 PN 1 PG 14 WC Behavioral Sciences; Zoology SC Behavioral Sciences; Zoology GA EW032 UT WOS:A1991EW03200009 ER PT B AU INSEL, TR WINSLOW, JT AF INSEL, TR WINSLOW, JT BE OLIVIER, B MOS, J SLANGEN, JL TI RAT PUP ULTRASONIC VOCALIZATIONS - AN ETHOLOGICALLY RELEVANT BEHAVIOR RESPONSIVE TO ANXIOLYTICS SO ANIMAL MODELS IN PSYCHOPHARMACOLOGY SE ADVANCES IN PHARMACOLOGICAL SCIENCES LA English DT Proceedings Paper CT POST-CONGRESS SATELLITE MEETING OF THE 11TH INTERNATIONAL CONGRESS OF PHARMACOLOGY : ANIMAL MODELS IN PSYCHOPHARMACOLOGY CY JUL 07-08, 1990 CL AMSTERDAM, NETHERLANDS SP DUPHAR RP INSEL, TR (reprint author), NIMH,CLIN SCI LAB,POB 289,POOLESVILLE,MD 20837, USA. NR 0 TC 34 Z9 34 U1 0 U2 0 PU BIRKHAUSER VERLAG PI BASEL PA BASEL BN 3-7643-2503-8 J9 ADV PHAR SC PY 1991 BP 15 EP 36 PG 22 WC Behavioral Sciences; Medicine, Research & Experimental; Pharmacology & Pharmacy SC Behavioral Sciences; Research & Experimental Medicine; Pharmacology & Pharmacy GA BT55R UT WOS:A1991BT55R00002 ER PT J AU SIMPSON, JL MILLS, J RHOADS, GG CUNNINGHAM, GC CONLEY, MR HOFFMAN, HJ AF SIMPSON, JL MILLS, J RHOADS, GG CUNNINGHAM, GC CONLEY, MR HOFFMAN, HJ TI GENETIC-HETEROGENEITY IN NEURAL-TUBE DEFECTS SO ANNALES DE GENETIQUE LA English DT Article DE NEURAL TUBE DEFECTS; ASSOCIATED ANOMALIES; HETEROGENEITY; EPIDEMIOLOGY ID ETIOLOGIC HETEROGENEITY; CLUES AB In 1985-1987, the authors attempted to ascertain all cases of confirmed neural tube defects (NTD) in California and Illinois, not only among live-born infants (postnatal) but also cases ascertained during pregnancy (prenatal). Mothers of both prenatal and postnatal NTD cases were interviewed within 5 months. Among postnatal NTD cases, 14.9% (45/303) had anomalies not ordinarily associated with NTD. The frequency of non-NTD related anomalies was 9.4% (5/53) in anencephaly, 0/3 in craniorachischisis, 22.9% (8/35) in encephalocele, 14.5% (27/186) in spina bifida, 20% (115) in multiple NTD cases and 19% (4/21) in other NTDs. However, relatively few postnatal NTD cases had known multiple malformation patterns; Meckel-Gruber syndrome was the most common, with 2 postnatal cases, and 3 additional prenatal cases. Maternal age, paternal age and birth order in postnatal cases were 26.7 +/- 5.4 SD, 28.9 +/- 5.8 and 2.8 +/- 1.8, respectively. These characteristics were similar in prenatal NTD cases (27.9 +/- 6.0, 30.1 +/- 6.3, 2.5 +/- 1.5, respectively). We also found no differences in parental ages among different types of NTD. Frequency of prior spontaneous abortion differed neither between postnatal NTD (9.3%) and postnatal controls (8.1%), nor between prenatal NTD (10.7%) and prenatal control (8.7%). Loss rates in the pregnancy immediately prior to the index NTD cases were not significantly higher than in control subjects. The high frequency of non-NTD associated malformations (14.9%) indicates the caution must be exercised before assuming that a given NTD case is polygenic-multifactorial in etiology, especially cases of encephalocele. C1 NICHHD,BETHESDA,MD 20892. CALIF PUBL HLTH FDN,BERKELEY,CA. RP SIMPSON, JL (reprint author), UNIV TENNESSEE CTR HLTH SCI,HLTH SCI CTR,DEPT OBSTET GYNECOL,MEMPHIS,TN 38163, USA. NR 14 TC 23 Z9 23 U1 0 U2 0 PU EXPANSION SCI FRANCAISE PI PARIS PA 31 BLVD LATOUR MAUBOURG, 75007 PARIS, FRANCE SN 0003-3995 J9 ANN GENET-PARIS JI Ann. Genet. PY 1991 VL 34 IS 3-4 BP 279 EP 286 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA HC265 UT WOS:A1991HC26500022 PM 1809238 ER PT J AU SAYPOL, JM ROTH, BJ COHEN, LG HALLETT, M AF SAYPOL, JM ROTH, BJ COHEN, LG HALLETT, M TI A THEORETICAL COMPARISON OF ELECTRIC AND MAGNETIC STIMULATION OF THE BRAIN SO ANNALS OF BIOMEDICAL ENGINEERING LA English DT Article DE MAGNETIC STIMULATION; ELECTRIC STIMULATION; ELECTRIC FIELD; CORTEX ID NERVOUS-SYSTEM; CORTEX; MUSCLE AB We present a theoretical comparison of the electric field produced in the brain by three modalities of transcranial stimulation of the cortex: magnetic stimulation, bifocal electric stimulation, and unifocal electric stimulation. The primary focus of this comparison is the focality and direction of the electric fields produced. A three-sphere model is used to represent the scalp, skull, and brain. All electric fields are calculated numerically. For magnetic stimulation we consider only a figure-of-eight coil. We find that magnetic stimulation produces the most focal field, while unifocal electric produces the least. Fields produced during magnetic stimulation are parallel to the head surface, while fields produced during electric stimulation have components both parallel and perpendicular to the head surface. The electric field produced by magnetic stimulation is shown to be insensitive to the skull conductivity, while that produced by electric stimulation is very sensitive to it. C1 NINCDS,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BLDG 13,BETHESDA,MD 20892. NINCDS,MED NEUROL BRANCH,HUMAN MOTOR CONTROL SECT,HUMAN CORT PHYSIOL UNIT,BETHESDA,MD 20892. RI Roth, Bradley/A-4920-2008 NR 34 TC 60 Z9 62 U1 0 U2 3 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0090-6964 J9 ANN BIOMED ENG JI Ann. Biomed. Eng. PY 1991 VL 19 IS 3 BP 317 EP 328 DI 10.1007/BF02584306 PG 12 WC Engineering, Biomedical SC Engineering GA GB404 UT WOS:A1991GB40400006 PM 1928873 ER PT J AU ROTH, BJ AF ROTH, BJ TI A COMPARISON OF 2 BOUNDARY-CONDITIONS USED WITH THE BIDOMAIN MODEL OF CARDIAC TISSUE SO ANNALS OF BIOMEDICAL ENGINEERING LA English DT Article DE BIDOMAIN MODEL; CARDIAC TISSUE; BOUNDARY CONDITIONS ID CYLINDRICAL BUNDLE; PROPAGATION; MUSCLE; SIMULATION; HEART AB In the bidomain model, two alternative sets of boundary conditions at the interface between cardiac tissue and a saline bath have been used. It is shown that these boundary conditions are equivalent if the length constant of the tissue in the direction transverse to the fibers is much larger than the radius of the individual cardiac cells. If this is not the case, the relative merits of the two boundary conditions are closely related to the question of the applicability of a continuum model, such as the bidomain model, to describe a discrete multicellular tissue. RP ROTH, BJ (reprint author), NIH, BIOMED ENGN & INSTRUMENTAT BRANCH, BLDG 13, ROOM 3W13, BETHESDA, MD 20892 USA. RI Roth, Bradley/A-4920-2008 NR 21 TC 31 Z9 31 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0090-6964 J9 ANN BIOMED ENG JI Ann. Biomed. Eng. PY 1991 VL 19 IS 6 BP 669 EP 678 DI 10.1007/BF02368075 PG 10 WC Engineering, Biomedical SC Engineering GA GY714 UT WOS:A1991GY71400002 PM 1781568 ER PT J AU FISHER, RI LONGO, DL DEVITA, VT HUBBARD, SM MILLER, TP YOUNG, RC AF FISHER, RI LONGO, DL DEVITA, VT HUBBARD, SM MILLER, TP YOUNG, RC TI LONG-TERM FOLLOW-UP OF PROMACE-CYTABOM IN NON-HODGKINS-LYMPHOMAS SO ANNALS OF ONCOLOGY LA English DT Article DE PROMACE-CYTABOM; NON-HODGKINS LYMPHOMAS INTERMEDIATE-GRADE HIGH-GRADE NON-HODGKINS LYMPHOMAS ID DIFFUSE HISTIOCYTIC LYMPHOMA; LARGE-CELL LYMPHOMA; CHEMOTHERAPY AB Initial results from studies with third-generation combination chemotherapy regimens for the treatment of aggressive non-Hodgkin's lymphomas (NHL) demonstrated complete remission (CR) rates higher than those reported with first-generation regimens. Long-term follow-up of these studies is required to know if the increased number of CRs translates into an increased number of long-term disease-free survivors. In this report, results obtained with one of the third-generation regimens, ProMACE (prednisone/methotrexate/doxorubicin/cyclophosphamide/etoposide)-CytaBOM (cytarabine/bleomycin/vincristine/methotrexate) are described. From 1981 to 1988, 193 patients with stage II, III, or IV aggressive NHL treated at the National Cancer Institute were randomly assigned to receive either ProMACE (day 1)-CytaBOM (day 8) or ProMACE (day 1)-MOPP (mechlorethamine/vincristine/procarbazine/prednisone) (day 8). With a median follow-up of 5 years, the CR rate was 86% for ProMACE-CytaBOM nu 74% for ProMACE-MOPP (P = 0.048). A plateau is also seen in the overall survival curves at 69% for ProMACE-CytaBOM nu 53% for ProMACE-MOPP (P = 0.046). The Southwest Oncology Group also conducted a phase II study of ProMACE-CytaBOM in 78 patients with stages II to IV intermediate- or high-grade NHL to determine the CR rate and long-term disease-free survival of this regimen in a national cooperative group setting. The CR rate was 65%. With a median follow-up of 38 months, disease-free survival is 50% at 3 years and overall survival is 57% at the same time point. Ultimate conclusions concerning the efficacy of this regimen await the results of the National High Priority Lymphoma Trial, which compares CHOP (cyclophosphamide/doxorubicin/vincristine/prednisone) nu m-BACOD (methotrexatebleomycin/doxorubin/cyclophosphamide/vincristine/dexamethasone) nu ProMACE-CytaBOM nu MACOP-B (methotrexate/doxorubicin/cyclophosphamide/vincristine/prednisone/bleomycin). C1 NCI,BETHESDA,MD 20892. SW ONCOL GRP,SAN ANTONIO,TX. NR 11 TC 20 Z9 20 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0923-7534 J9 ANN ONCOL JI Ann. Oncol. PD JAN PY 1991 VL 2 SU 1 BP 33 EP 35 PG 3 WC Oncology SC Oncology GA EV300 UT WOS:A1991EV30000007 PM 1710487 ER PT J AU FRAKER, DL TRAVIS, WD MERENDINO, JJ ZIMERING, MB STREETEN, EA WEINSTEIN, LS MARX, SJ SPIEGEL, AM AURBACH, GD DOPPMAN, JL NORTON, JA AF FRAKER, DL TRAVIS, WD MERENDINO, JJ ZIMERING, MB STREETEN, EA WEINSTEIN, LS MARX, SJ SPIEGEL, AM AURBACH, GD DOPPMAN, JL NORTON, JA TI LOCALLY RECURRENT PARATHYROID NEOPLASMS AS A CAUSE FOR RECURRENT AND PERSISTENT PRIMARY HYPERPARATHYROIDISM SO ANNALS OF SURGERY LA English DT Article ID HYPER-PARATHYROIDISM; UNDERGONE SURGERY; CARCINOMA; REOPERATIONS; LOCALIZATION; ADENOMAS; MANAGEMENT; STRATEGY AB Between 1982 and 1989, 145 patients underwent operations for persistent or recurrent primary hyperparathyroidism (HPT). At re-exploration, 15 patients (10.3%) were found to have locally recurrent parathyroid tumors (11 patients with adenoma and 4 with carcinoma). These 15 patients had 28 previous operations at outside institutions for HPT. Patients with locally recurrent HPT secondary to adenoma had a longer disease-free interval than patients with locally recurrent carcinoma. At the time of evaluation at the National Institutes of Health (NIH) for recurrent or persistent HPT, each patient was symptomatic and patients with carcinoma had significantly more symptoms and higher serum levels of calcium and parathyroid hormone than patients with adenoma. Locally recurrent parathyroid neoplasm was correctly localized by preoperative testing in 14 of 15 patients. These 15 patients underwent 18 reoperations at NIH for excision of locally recurrent parathyroid tumors. Following the final reoperation (two patients had more than one procedure), each patient had normal serum levels of calcium. In addition each patient remains biochemically cured (based on normal serum calcium level), with a median follow-up interval of 21 months. Local recurrence of parathyroid adenoma comprises a small but significant proportion of cases of recurrent or persistent HPT and can be indistinguishable from parathyroid carcinoma. Findings suggestive of carcinoma include shorter disease-free interval, higher serum levels of calcium and parathyroid hormone, and histologic appearance. Whether the locally recurrent parathyroid neoplasm is benign or malignant, aggressive surgery can control serum levels of calcium in these patients with acceptable rates of morbidity. C1 NCI,SURG BRANCH,SURG METAB SECT,BLDG 10,ROOM 2B07,BETHESDA,MD 20892. NCI,PATHOL LAB,BETHESDA,MD 20892. NIDDKD,MOLEC PATHOPHYSIOL BRANCH,BETHESDA,MD. NIDDKD,METAB DIS BRANCH,BETHESDA,MD. NIH,CTR CLIN,DEPT RADIOL,BETHESDA,MD 20892. RI Weinstein, Lee/I-5575-2015 OI Weinstein, Lee/0000-0002-1899-5152; NR 25 TC 26 Z9 27 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0003-4932 J9 ANN SURG JI Ann. Surg. PD JAN PY 1991 VL 213 IS 1 BP 58 EP 65 DI 10.1097/00000658-199101000-00010 PG 8 WC Surgery SC Surgery GA ER539 UT WOS:A1991ER53900010 PM 1985539 ER PT J AU SWAIN, JA MCDONALD, TJ BALABAN, RS ROBBINS, RC AF SWAIN, JA MCDONALD, TJ BALABAN, RS ROBBINS, RC TI METABOLISM OF THE HEART AND BRAIN DURING HYPOTHERMIC CARDIOPULMONARY BYPASS SO ANNALS OF THORACIC SURGERY LA English DT Article ID NUCLEAR MAGNETIC-RESONANCE; INTRACELLULAR PH; OXYGEN-CONSUMPTION; RAT-BRAIN AB The alterations in tissue metabolism induced by hypothermic cardiopulmonary bypass are not completely known. Phosphorus-31 nuclear magnetic resonance spectroscopy was used to determine the effect of hypothermic cardiopulmonary bypass on energy states of intracellular pH of the heart and brain. Sheep were instrumented for cardiopulmonary bypass and had a radiofrequency coil placed over either the heart or skull. The animals were placed in a 4.7-T magnet at 37-degrees-C and spectra obtained. The animals were cooled on cardiopulmonary bypass to either 26-degrees-C (n = 17) or 18-degrees-C (n = 14) for brain studies and to 26-degrees-C (n = 12) for heart studies. Hypothermia increased the phosphocreatine/adenosine triphosphate ratio in the heart (2.38 +/- 0.23 versus 3.18 +/- 0.37, 37-degrees versus 26-degrees-C, respectively, p = 0.03). The brain phosphocreatine/adenosine triphosphate ratio increased from 1.70 +/- 0.09 at 37-degrees-C to 2.00 +/- 0.12 at 26-degrees-C (p = 0.009) and 2.10 +/- 0.07 at 18-degrees-C (p = 0.0001). Intracellular pH increased during hypothermia (heart: 7.05 +/- 0.02 to 7.18 +/- 0.02, 37-degrees versus 26-degrees-C, p = 0.0001; and brain; 7.07 +/- 0.02 versus 7.32 +/- 0.02, 37-degrees versus 18-degrees-C, p = 0.0001). The adenosine triphosphate resonance position is known to be sensitive to magnesium binding as well as temperature and was shifted upfield (p < 0.01) in both the heart and brain. This effect could be totally explained by the temperature dependence of this process. It is concluded that deep hypothermia increases the energy state and the intracellular pH of both the heart and the brain. The elevated intracellular pH and the increase in tissue energy state may partially explain the beneficial effects of hypothermia on organ tolerance to ischemia. C1 NHLBI,SURG BRANCH,BETHESDA,MD 20892. NHLBI,CARDIAC ENERGET LAB,BETHESDA,MD 20892. NR 19 TC 39 Z9 42 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0003-4975 J9 ANN THORAC SURG JI Ann. Thorac. Surg. PD JAN PY 1991 VL 51 IS 1 BP 105 EP 109 PG 5 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA EQ818 UT WOS:A1991EQ81800023 PM 1985546 ER PT J AU LONDON, J AF LONDON, J TI BACTERIAL ADHESINS SO ANNUAL REPORTS IN MEDICINAL CHEMISTRY LA English DT Review ID UROPATHOGENIC ESCHERICHIA-COLI; MANNOSE-RESISTANT HEMAGGLUTINATION; MYCOPLASMA-PNEUMONIAE; PSEUDOMONAS-AERUGINOSA; STREPTOCOCCUS-SANGUIS; RECEPTOR-BINDING; NUCLEOTIDE-SEQUENCE; EPITHELIAL-CELLS; TYPE-1 FIMBRIAE; MONOCLONAL-ANTIBODIES RP NIDR, MICROBIAL ECOL LAB, BETHESDA, MD 20892 USA. NR 90 TC 1 Z9 1 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0065-7743 J9 ANNU REP MED CHEM JI Annu. Rep. Med. Chem. PY 1991 VL 26 BP 239 EP 247 PG 9 WC Chemistry, Medicinal; Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GL356 UT WOS:A1991GL35600025 ER PT J AU EGUCHI, Y ITOH, T TOMIZAWA, J AF EGUCHI, Y ITOH, T TOMIZAWA, J TI ANTISENSE RNA SO ANNUAL REVIEW OF BIOCHEMISTRY LA English DT Review DE RNA-RNA INTERACTION; COLE1; RNA-I; RNA-II; ROM ID COLE1 PLASMID REPLICATION; COMPLEMENTARY-OLIGONUCLEOTIDE BINDING; COPY NUMBER CONTROL; HELIX-COIL TRANSITION; ANTI-SENSE RNA; GENE-EXPRESSION; PRIMER FORMATION; ROM PROTEIN; TERMINAL MISMATCHES; SECONDARY STRUCTURE C1 NIDDKD,MOLEC BIOL LAB,BETHESDA,MD 20892. OSAKA UNIV,FAC SCI,DEPT BIOL,TOYONAKA,OSAKA 560,JAPAN. NATL INST GENET,MISHIMA,SHIZUOKA 411,JAPAN. NR 144 TC 252 Z9 257 U1 0 U2 5 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4154 J9 ANNU REV BIOCHEM JI Annu. Rev. Biochem. PY 1991 VL 60 BP 631 EP 652 DI 10.1146/annurev.biochem.60.1.631 PG 22 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FV909 UT WOS:A1991FV90900019 PM 1715680 ER PT J AU CLORE, GM GRONENBORN, AM AF CLORE, GM GRONENBORN, AM TI 2-DIMENSIONAL, 3-DIMENSIONAL, AND 4-DIMENSIONAL NMR METHODS FOR OBTAINING LARGER AND MORE PRECISE 3-DIMENSIONAL STRUCTURES OF PROTEINS IN SOLUTION SO ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY LA English DT Review DE PROTEIN STRUCTURE IN SOLUTION; MULTIDIMENSIONAL NMR; NUCLEAR OVERHAUSER EFFECT ID NUCLEAR-MAGNETIC-RESONANCE; RESTRAINED MOLECULAR-DYNAMICS; INTERPROTON DISTANCE RESTRAINTS; TWO-DIMENSIONAL NMR; OVERHAUSER ENHANCEMENT SPECTROSCOPY; POTATO CARBOXYPEPTIDASE INHIBITOR; SINGLE ZINC FINGER; SECONDARY STRUCTURE; SOLUTION CONFORMATION; COUPLING-CONSTANTS RP CLORE, GM (reprint author), NIDDKD,CHEM PHYS LAB,BLDG 2,BETHESDA,MD 20892, USA. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 120 TC 119 Z9 119 U1 0 U2 4 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0084-6589 J9 ANNU REV BIOPHYS BIO PY 1991 VL 20 BP 29 EP 63 DI 10.1146/annurev.biophys.20.1.29 PG 35 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FQ360 UT WOS:A1991FQ36000002 PM 1651086 ER PT J AU KARLIN, S BUCHER, P BRENDEL, V ALTSCHUL, SF AF KARLIN, S BUCHER, P BRENDEL, V ALTSCHUL, SF TI STATISTICAL-METHODS AND INSIGHTS FOR PROTEIN AND DNA-SEQUENCES SO ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY LA English DT Review DE SEQUENCE ANALYSIS; SEQUENCE STATISTICS; SEQUENCE ALIGNMENTS; CHARGE DISTRIBUTION IN PROTEIN SEQUENCES; RESIDUE ASSOCIATIONS ID AMINO-ACID SEQUENCE; IMMUNOGLOBULIN-KAPPA-GENE; CHARGE CONFIGURATIONS; PATTERN-RECOGNITION; NUCLEOTIDE-SEQUENCES; MOLECULAR SEQUENCES; BINDING PROTEINS; LEUCINE ZIPPER; CODON USAGE; ALIGNMENT C1 NATL LIB MED, NATL CTR BIOTECHNOL, BETHESDA, MD 20894 USA. RP STANFORD UNIV, DEPT MATH, STANFORD, CA 94305 USA. FU NIGMS NIH HHS [GM10452-26, GM39907-02] NR 104 TC 60 Z9 60 U1 0 U2 4 PU ANNUAL REVIEWS PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 USA SN 0084-6589 J9 ANNU REV BIOPHYS BIO PY 1991 VL 20 BP 175 EP 203 DI 10.1146/annurev.biophys.20.1.175 PG 29 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FQ360 UT WOS:A1991FQ36000008 PM 1867715 ER PT J AU DRAKE, JW AF DRAKE, JW TI SPONTANEOUS MUTATION SO ANNUAL REVIEW OF GENETICS LA English DT Review DE SPONTANEOUS MUTATION; DNA BASE MISPAIRING; DIRECTED MUTATION; EVOLUTION OF MUTATION RATES; COMPLEX MUTATIONS; DISLOCATION MUTAGENESIS ID BASE-PAIR MISMATCHES; CARCINOGEN-MODIFIED BASES; HERPES-SIMPLEX VIRUS; ESCHERICHIA-COLI; DNA-POLYMERASE; DODECANUCLEOTIDE DUPLEXES; DIRECTED MUTATION; SUBSTITUTION MUTATIONS; MAGNETIC-RESONANCE; MUTAGENESIS RP DRAKE, JW (reprint author), NIEHS, MOLEC GENET LAB, RES TRIANGLE PK, NC 27709 USA. NR 107 TC 129 Z9 132 U1 2 U2 13 PU ANNUAL REVIEWS PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 USA SN 0066-4197 J9 ANNU REV GENET JI Annu. Rev. Genet. PY 1991 VL 25 BP 125 EP 146 PG 22 WC Genetics & Heredity SC Genetics & Heredity GA GX174 UT WOS:A1991GX17400006 PM 1812804 ER PT J AU STAUDT, LM LENARDO, MJ AF STAUDT, LM LENARDO, MJ TI IMMUNOGLOBULIN GENE-TRANSCRIPTION SO ANNUAL REVIEW OF IMMUNOLOGY LA English DT Review DE ENHANCER; PROMOTER; LYMPHOCYTE-B; DNA-BINDING PROTEIN ID HEAVY-CHAIN PROMOTER; NF-KAPPA-B; CELL-TYPE-SPECIFICITY; ENHANCER-BINDING-PROTEIN; DNA-SEQUENCE ELEMENTS; SMALL NUCLEAR-RNA; HISTONE H2B GENE; IGH ENHANCER; CONSERVED SEQUENCE; CONSTANT-REGION AB Transcriptional regulation of the immunoglobulin (Ig) genes is one of the best-studied systems for understanding tissue-restricted gene control. Dissection of the Ig genes reveals that each contains multiple regulatory elements that are preferentially active in B lymphocytes. These include a promoter and one or more enhancer elements. Biochemical analysis and cloning of the genes for transactivator proteins has uncovered a variety of distinct proteins that are often members of gene families. The transactivators themselves are under complex regulation at the transcriptional level as well as by interactions with other proteins in the cell. It appears that the coordinate action of a select constellation of these transactivators conveys the information for tissue-specific expression of the Ig genes. C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. RP STAUDT, LM (reprint author), NCI,METAB BRANCH,BETHESDA,MD 20892, USA. NR 140 TC 285 Z9 286 U1 0 U2 0 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0732-0582 J9 ANNU REV IMMUNOL JI Annu. Rev. Immunol. PY 1991 VL 9 BP 373 EP 398 PG 26 WC Immunology SC Immunology GA FF998 UT WOS:A1991FF99800014 PM 1910683 ER PT J AU RAVETCH, JV KINET, JP AF RAVETCH, JV KINET, JP TI FC-RECEPTORS SO ANNUAL REVIEW OF IMMUNOLOGY LA English DT Review DE CELL SURFACE; SIGNALING, ALLERGY; IGG; IGE ID BASOPHILIC LEUKEMIA-CELLS; NATURAL-KILLER CELLS; AFFINITY IGE RECEPTOR; RAT MAST-CELLS; HUMAN MONONUCLEAR PHAGOCYTES; HUMAN IMMUNOGLOBULIN-E; SINGLE AMINO-ACID; MONOCLONAL-ANTIBODY; HISTAMINE-RELEASE; GAMMA-RECEPTOR AB Recent advances in the structural analysis of the genes and proteins for immunoglobulin Fc domain receptors have provided a molecular characterization of this complex family. The wide cellular distribution of these receptors and their functional heterogeneity are reflected in the diversity of molecules which bind antibody and immune complexes. The detailed analysis of the IgG and IgE Fc receptors has indicated that these molecules have evolved from a common precursor through gene duplication. Similarities among these receptors, in both structure and function have emerged. Thus, the Fc receptors provide an example of a class of molecules in which conserved domains are combined with divergent sequences to yield a diversity of function. C1 NIAID,MOLEC ALLERGY & IMMUNOL SECT,ROCKVILLE,MD 20852. RP RAVETCH, JV (reprint author), MEM SLOAN KETTERING CANC CTR,DEWITT WALLACE RES LABS,1275 YORK AVE,NEW YORK,NY 10021, USA. NR 162 TC 1334 Z9 1345 U1 3 U2 51 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0732-0582 J9 ANNU REV IMMUNOL JI Annu. Rev. Immunol. PY 1991 VL 9 BP 457 EP 492 PG 36 WC Immunology SC Immunology GA FF998 UT WOS:A1991FF99800017 PM 1910686 ER PT J AU OPPENHEIM, JJ ZACHARIAE, COC MUKAIDA, N MATSUSHIMA, K AF OPPENHEIM, JJ ZACHARIAE, COC MUKAIDA, N MATSUSHIMA, K TI PROPERTIES OF THE NOVEL PROINFLAMMATORY SUPERGENE INTERCRINE CYTOKINE FAMILY SO ANNUAL REVIEW OF IMMUNOLOGY LA English DT Review DE CYTOKINES; INTERLEUKINS; PLATELET FACTOR-IV; BETA THROMBOGLOBULIN; INFLAMMATION ID NEUTROPHIL CHEMOTACTIC FACTOR; MACROPHAGE INFLAMMATORY PROTEIN-1; BLOOD MONONUCLEAR LEUKOCYTES; TUMOR NECROSIS FACTOR; GROWTH STIMULATORY ACTIVITY; HUMAN DERMAL FIBROBLASTS; HUMAN-ENDOTHELIAL CELLS; ALPHA-GRANULE PROTEINS; PLATELET BASIC-PROTEIN; ACTIVATING FACTOR MCAF AB A family consisting of at least ten distinct novel 8-10 kd cytokines has been identified over the past 12 years. These cytokines exhibit from 20 to 45% homology in amino acid sequence, are probably all basic heparin-binding polypeptides, and have proinflammatory and reparative activities. The cDNA for these cytokines are characterized by conserved single open reading frames, typical signal sequences in the 5' region, and AT rich sequences in the 3' untranslated regions. Those human cytokines known as interleukin 8, platelet factor 4, beta thromboglobulin, IP-10 and melanoma growth stimulating factor or GRO can be assigned to a subfamily based on their location on chromosome 4 and unique structural features, whereas the second subset consisting of LD78, ACT-2, I-309, RANTES, and macrophage chemotactic and activating factor (MCAF) are all closely linked on human chromosome 17. In this review we have summarized and discussed the available information concerning the regulation and structure of the genes, the structure and biochemical properties of the polypeptide products, their receptors, signal transduction, cell sources, and in vitro as well as in vivo activities of these cytokines. RP OPPENHEIM, JJ (reprint author), NCI,BIOL RESPONSE MODIFIERS PROGRAM,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21702, USA. RI Mukaida, Naofumi/D-7623-2011 OI Mukaida, Naofumi/0000-0002-4193-1851 NR 152 TC 1984 Z9 1998 U1 2 U2 24 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0732-0582 J9 ANNU REV IMMUNOL JI Annu. Rev. Immunol. PY 1991 VL 9 BP 617 EP 648 PG 32 WC Immunology SC Immunology GA FF998 UT WOS:A1991FF99800022 PM 1910690 ER PT J AU MCFARLIN, DE BLATTNER, WA AF MCFARLIN, DE BLATTNER, WA TI NON-AIDS RETROVIRAL INFECTIONS IN HUMANS SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE HTLV-I; HTLV-II; ADULT T-CELL LEUKEMIA; HTLV-I-RELATED MYELOPATHY; TROPICAL SPASTIC PARAPLEGIA; POLYMYOSITIS ID VIRUS TYPE-I; CELL LEUKEMIA-VIRUS; MULTIPLE-SCLEROSIS PATIENTS; TROPICAL SPASTIC PARAPARESIS; HTLV-I; PCR ANALYSIS; TRANSMISSION; DNA; MYELOPATHY; LYMPHOMA AB In the decade since the first human retrovirus was identified, one form of leukemia and a chronic neurological disorder have been shown to be related to this agent. A spectrum of lymphomas, additional inflammatory disorders such as polymyositis, and possibly other neurological diseases also have been linked to HTLV-I infection. Although the pathogenesis of these disorders remains to be defined, these exciting developments coupled with the discovery that AIDS is a retroviral-induced disease indicate that human retroviruses are responsible for a variety of human disorders and suggest that other agents such as members of the foamy virus subfamily, as well as yet unidentified human retroviruses, may produce other human diseases. C1 NCI,DEPT CANC EPIDEMIOL,VIRAL EPIDEMIOL SECT,BETHESDA,MD 20892. RP MCFARLIN, DE (reprint author), NINCDS,NEUROIMMUNOL BRANCH,BETHESDA,MD 20892, USA. NR 44 TC 19 Z9 20 U1 0 U2 0 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1991 VL 42 BP 97 EP 105 DI 10.1146/annurev.me.42.020191.000525 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA FE086 UT WOS:A1991FE08600010 PM 2035998 ER PT J AU FREIFELD, AG OSTROVE, JM AF FREIFELD, AG OSTROVE, JM TI RESISTANCE OF VIRUSES TO ANTIVIRAL DRUGS SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE HERPES SIMPLEX VIRUS; VARICELLA ZOSTER VIRUS; CYTOMEGALOVIRUS ID HERPES-SIMPLEX VIRUS; ACQUIRED IMMUNODEFICIENCY SYNDROME; ALTERED SUBSTRATE-SPECIFICITY; VARICELLA-ZOSTER VIRUS; THYMIDINE KINASE; CYTOMEGALO-VIRUS; GENITAL HERPES; ORAL ACYCLOVIR; IMMUNOCOMPROMISED PATIENTS; MOLECULAR-BASIS AB In recent years, substantial advances in antiviral therapy have been made; however, in the immunocompromised host, antiviral drug resistance is becoming an issue of increasing clinical importance. Understanding the mechanism of action of antiviral agents, especially those used to treat herpesvirus infections, may enable us to design new therapeutic agents and better treatment regimens to deal with antiviral drug resistance. RP FREIFELD, AG (reprint author), NIAID,CLIN INVEST LAB,MED VIROL SECT,BETHESDA,MD 20892, USA. NR 44 TC 5 Z9 5 U1 0 U2 0 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1991 VL 42 BP 247 EP 259 DI 10.1146/annurev.med.42.1.247 PG 13 WC Medicine, General & Internal SC General & Internal Medicine GA FE086 UT WOS:A1991FE08600025 PM 1852148 ER PT J AU PASTAN, I GOTTESMAN, MM AF PASTAN, I GOTTESMAN, MM TI MULTIDRUG RESISTANCE SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE P-GLYCOPROTEIN; DOXORUBICIN; VINBLASTINE; CANCER; GENE THERAPY; VERAPAMIL ID P-GLYCOPROTEIN GENE; TUMOR-CELLS; EXPRESSION; MDR1; TRANSPORT; DAUNOMYCIN; VESICLES; 170-KDA; TISSUES; CANCER AB Laboratory investigations indicate that cancer cells can become simultaneously resistant to many different chemotherapeutic drugs that are natural products via the expression of an energy-dependent drug efflux pump. This multidrug transporter is a plasma membrane glycoprotein encoded in the human by the MDR1 gene. Recent clinical studies indicate that expression of the multidrug transporter plays a major role in the intrinsic and acquired resistance to chemotherapy of many human cancers. Strategies aimed at inactivating this drug efflux pump may have significant impact on the treatment of human cancer. C1 NCI,CELL BIOL LAB,BETHESDA,MD 20892. RP PASTAN, I (reprint author), NCI,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 25 TC 240 Z9 243 U1 3 U2 11 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1991 VL 42 BP 277 EP 286 DI 10.1146/annurev.med.42.1.277 PG 10 WC Medicine, General & Internal SC General & Internal Medicine GA FE086 UT WOS:A1991FE08600028 PM 2035973 ER PT J AU LUFT, FC MCCARRON, DA AF LUFT, FC MCCARRON, DA TI HETEROGENEITY OF HYPERTENSION - THE DIVERSE ROLE OF ELECTROLYTE INTAKE SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE BLOOD PRESSURE; SODIUM; POTASSIUM; CALCIUM; MAGNESIUM; CHLORIDE; DIET; NUTRITION ID SALT-SENSITIVE HYPERTENSION; CALCIUM-REGULATING HORMONES; BLOOD-PRESSURE; DEPENDENT HYPERTENSION; PARATHYROID FUNCTION; SODIUM RESTRICTION; DIETARY POTASSIUM; CONTROLLED TRIAL; UNITED-STATES; METABOLISM AB Nonpharmacologic approaches to treating patients with hypertension are both medically worthwhile and laudable as a public health goal. Reduced salt intake has been the primary dietary measure, but now attention is being directed at potassium, calcium, magnesium, and the anions accompanying these cations. The importance of total caloric intake and of confounding dietary variables such as alcohol consumption are also being acknowledged. It is important to recognize that nutrients are not ingested in isolation, but as interactive constituents of a total diet. This principle may account for some of the heterogeneity of the human blood pressure response to variations of individual electrolyte intake. Moreover, failure to appreciate the heterogeneity and interactive influences may result in other than the desired effect in the population at risk. C1 OREGON HLTH SCI UNIV,NIDDK,CLIN NUTR RES UNIT,NDB INST NUTR & CARDIOVASC RES,PORTLAND,OR 97201. RP LUFT, FC (reprint author), UNIV ERLANGEN NURNBERG,LEHRSTUHL INNERE MED,W-8520 ERLANGEN,GERMANY. OI Luft, Friedrich/0000-0002-8635-1199 NR 58 TC 15 Z9 15 U1 0 U2 1 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1991 VL 42 BP 347 EP 355 DI 10.1146/annurev.med.42.1.347 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA FE086 UT WOS:A1991FE08600034 PM 2035979 ER PT J AU TAYLOR, SI ACCILI, D CAMA, A IMANO, E KADOWAKI, H KADOWAKI, T AF TAYLOR, SI ACCILI, D CAMA, A IMANO, E KADOWAKI, H KADOWAKI, T TI UNUSUAL FORMS OF INSULIN RESISTANCE SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE DIABETES MELLITUS; INSULIN RECEPTORS; HYPERANDROGENISM; ACANTHOSIS NIGRICANS ID RECEPTOR GENE; HUMAN-DISEASE; PIMA-INDIANS; AUTOANTIBODIES; HYPOGLYCEMIA; DISORDERS; PATIENT AB Insulin resistance is an important feature in the pathogenesis of non-insulin-dependent diabetes mellitus. Some rare patients have syndromes associated with a severe form of insulin resistance, usually involving acanthosis nigricans and, in premenopausal females, hyperandrogenism. This review discusses two of the causes of insulin resistance: mutations in the insulin receptor gene and autoantibodies directed against the insulin receptor. RP TAYLOR, SI (reprint author), NIDDKD,DIABET BRANCH,BETHESDA,MD 20892, USA. NR 19 TC 11 Z9 11 U1 0 U2 0 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1991 VL 42 BP 373 EP 379 DI 10.1146/annurev.med.42.1.373 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA FE086 UT WOS:A1991FE08600036 PM 2035982 ER PT J AU CROEN, KD STRAUS, SE AF CROEN, KD STRAUS, SE TI VARICELLA-ZOSTER VIRUS LATENCY SO ANNUAL REVIEW OF MICROBIOLOGY LA English DT Review DE HERPESVIRUS; REACTIVATION; TRANSCRIPTION; GENES ID HERPES-SIMPLEX VIRUS; HUMAN TRIGEMINAL GANGLIA; GUINEA-PIGS; NONIMMUNOCOMPROMISED CHILDREN; MACROMOLECULAR-SYNTHESIS; EXPERIMENTAL-INFECTION; THORACIC GANGLIA; GENITAL HERPES; INVIVO MODEL; VIRAL-DNA C1 NIAID,CLIN INVEST LAB,MED VIROL SECT,BETHESDA,MD 20892. RP CROEN, KD (reprint author), UNIV CINCINNATI,COLL MED,DEPT MED,CINCINNATI,OH 45267, USA. NR 83 TC 22 Z9 22 U1 0 U2 0 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4227 J9 ANNU REV MICROBIOL JI Annu. Rev. Microbiol. PY 1991 VL 45 BP 265 EP 282 PG 18 WC Microbiology SC Microbiology GA GH814 UT WOS:A1991GH81400011 PM 1660255 ER PT J AU HUFF, J HASEMAN, J RALL, D AF HUFF, J HASEMAN, J RALL, D TI SCIENTIFIC CONCEPTS, VALUE, AND SIGNIFICANCE OF CHEMICAL CARCINOGENESIS STUDIES SO ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY LA English DT Review DE LONG-TERM STUDIES; PESTICIDES; HUMAN RISKS; CANCER; SYNTHETIC AND NATURAL CHEMICALS ID LONG-TERM CARCINOGENICITY; B6C3F1 MOUSE; ACTIVATED ONCOGENES; RAS PROTOONCOGENE; TUMORS; BENIGN RP HUFF, J (reprint author), NIEHS,RES TRIANGLE PK,NC 27709, USA. NR 54 TC 94 Z9 94 U1 0 U2 3 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0362-1642 J9 ANNU REV PHARMACOL JI Annu. Rev. Pharmacol. Toxicol. PY 1991 VL 31 BP 621 EP 652 PG 32 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA FH228 UT WOS:A1991FH22800024 PM 2064387 ER PT J AU LONDON, RE AF LONDON, RE TI METHODS FOR MEASUREMENT OF INTRACELLULAR MAGNESIUM - NMR AND FLUORESCENCE SO ANNUAL REVIEW OF PHYSIOLOGY LA English DT Review DE FURAPTRA; APTRA; ATP; MAGNESIUM INDICATORS; FLUORINE NMR ID NUCLEAR MAGNETIC-RESONANCE; CYTOSOLIC FREE MAGNESIUM; ISCHEMIC RAT-HEART; ADENOSINE 5'-TRIPHOSPHATE; F-19 NMR; P-31-NMR SPECTROSCOPY; CELLS; MG-2+; ATP; INDICATORS RP LONDON, RE (reprint author), NIEHS,MOLEC BIOPHYS LAB,BOX 12233,RES TRIANGLE PK,NC 27709, USA. NR 43 TC 136 Z9 138 U1 1 U2 16 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4278 J9 ANNU REV PHYSIOL JI Annu. Rev. Physiol. PY 1991 VL 53 BP 241 EP 258 DI 10.1146/annurev.physiol.53.1.241 PG 18 WC Physiology SC Physiology GA FA889 UT WOS:A1991FA88900013 PM 2042961 ER PT J AU MURPHY, E FREUDENRICH, CC LIEBERMAN, M AF MURPHY, E FREUDENRICH, CC LIEBERMAN, M TI CELLULAR MAGNESIUM AND NA/MG EXCHANGE IN HEART-CELLS SO ANNUAL REVIEW OF PHYSIOLOGY LA English DT Review DE FURAPTRA/MAG-FURA-2; ION SELECTIVE MICROELECTRODES; NMR; MG-TRANSPORT ID CYTOSOLIC FREE MAGNESIUM; SQUID GIANT-AXONS; INTRACELLULAR FREE MAGNESIUM; X-RAY-MICROANALYSIS; RED BLOOD-CELLS; SARCOPLASMIC-RETICULUM; CARDIAC MYOCYTES; MYOCARDIAL ISCHEMIA; MAGNETIC-RESONANCE; VENTRICULAR CELLS C1 DUKE UNIV,MED CTR,DEPT CELL BIOL,DIV PHYSIOL,DURHAM,NC 27710. RP MURPHY, E (reprint author), NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709, USA. NR 79 TC 126 Z9 126 U1 0 U2 1 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4278 J9 ANNU REV PHYSIOL JI Annu. Rev. Physiol. PY 1991 VL 53 BP 273 EP 287 DI 10.1146/annurev.physiol.53.1.273 PG 15 WC Physiology SC Physiology GA FA889 UT WOS:A1991FA88900015 PM 2042963 ER PT S AU ROBERTS, K SHEVACH, EM AF ROBERTS, K SHEVACH, EM BE Edelson, RL TI IMMUNOREGULATORY ROLE OF GAMMA-DELTA-T CELLS SO ANTIGEN AND CLONE-SPECIFIC IMMUNOREGULATION SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON ANTIGEN AND CLONE-SPECIFIC IMMUNOREGULATION CY OCT 22-24, 1990 CL NEW YORK ACAD SCI, NEW YORK, NY SP NEW YORK ACAD SCI THERAKOS, YALE UNIV, NIAID, NIDDKD, AMER CYANAMID, FISONS PHARM, GENENTECH, MERCK SHARP & DOHME RES LABS, MONSANTO HO NEW YORK ACAD SCI RP ROBERTS, K (reprint author), NIAID,IMMUNOL LAB,BLDG 10,ROOM 11N315,BETHESDA,MD 20892, USA. NR 0 TC 8 Z9 8 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK SN 0077-8923 BN 0-89766-688-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 636 BP 1 EP 8 DI 10.1111/j.1749-6632.1991.tb33432.x PG 8 WC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology SC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology GA BW41E UT WOS:A1991BW41E00001 PM 1724360 ER PT S AU ZACHARCHUK, CM MERCEP, M ASHWELL, JD AF ZACHARCHUK, CM MERCEP, M ASHWELL, JD BE Edelson, RL TI THYMOCYTE ACTIVATION AND DEATH - A MECHANISM FOR MOLDING THE T-CELL REPERTOIRE SO ANTIGEN AND CLONE-SPECIFIC IMMUNOREGULATION SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON ANTIGEN AND CLONE-SPECIFIC IMMUNOREGULATION CY OCT 22-24, 1990 CL NEW YORK ACAD SCI, NEW YORK, NY SP NEW YORK ACAD SCI THERAKOS, YALE UNIV, NIAID, NIDDKD, AMER CYANAMID, FISONS PHARM, GENENTECH, MERCK SHARP & DOHME RES LABS, MONSANTO HO NEW YORK ACAD SCI RP ZACHARCHUK, CM (reprint author), NCI,BIOL RESPONSE MODIFIERS PROGRAM,BLDG 10,ROOM 13N-268,BETHESDA,MD 20892, USA. NR 0 TC 25 Z9 25 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK SN 0077-8923 BN 0-89766-688-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 636 BP 52 EP 70 DI 10.1111/j.1749-6632.1991.tb33438.x PG 19 WC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology SC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology GA BW41E UT WOS:A1991BW41E00007 PM 1793231 ER PT S AU SEGAL, DM QIAN, JH ANDREW, SM TITUS, JA MEZZANZANICA, D GARRIDO, MA WUNDERLICH, JR AF SEGAL, DM QIAN, JH ANDREW, SM TITUS, JA MEZZANZANICA, D GARRIDO, MA WUNDERLICH, JR BE Edelson, RL TI CYTOKINE RELEASE BY PERIPHERAL-BLOOD LYMPHOCYTES TARGETED WITH BISPECIFIC ANTIBODIES, AND ITS ROLE IN BLOCKING TUMOR-GROWTH SO ANTIGEN AND CLONE-SPECIFIC IMMUNOREGULATION SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON ANTIGEN AND CLONE-SPECIFIC IMMUNOREGULATION CY OCT 22-24, 1990 CL NEW YORK ACAD SCI, NEW YORK, NY SP NEW YORK ACAD SCI THERAKOS, YALE UNIV, NIAID, NIDDKD, AMER CYANAMID, FISONS PHARM, GENENTECH, MERCK SHARP & DOHME RES LABS, MONSANTO HO NEW YORK ACAD SCI RP SEGAL, DM (reprint author), NCI,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4B17,BETHESDA,MD 20892, USA. OI Mezzanzanica, Delia/0000-0002-9664-6871 NR 0 TC 4 Z9 4 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK SN 0077-8923 BN 0-89766-688-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 636 BP 288 EP 294 DI 10.1111/j.1749-6632.1991.tb33459.x PG 7 WC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology SC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology GA BW41E UT WOS:A1991BW41E00028 PM 1793216 ER PT S AU CHEN, HT WANG, XQ AF CHEN, HT WANG, XQ BE Edelson, RL TI PREPARATION AND COMBINING SITE CHARACTERIZATION OF MOUSE MONOCLONAL ANTI-B BLOOD-GROUP SUBSTANCES SO ANTIGEN AND CLONE-SPECIFIC IMMUNOREGULATION SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON ANTIGEN AND CLONE-SPECIFIC IMMUNOREGULATION CY OCT 22-24, 1990 CL NEW YORK ACAD SCI, NEW YORK, NY SP NEW YORK ACAD SCI THERAKOS, YALE UNIV, NIAID, NIDDKD, AMER CYANAMID, FISONS PHARM, GENENTECH, MERCK SHARP & DOHME RES LABS, MONSANTO HO NEW YORK ACAD SCI RP CHEN, HT (reprint author), NIH,BLDG 10,RM D03,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK SN 0077-8923 BN 0-89766-688-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 636 BP 373 EP 374 DI 10.1111/j.1749-6632.1991.tb33469.x PG 2 WC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology SC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology GA BW41E UT WOS:A1991BW41E00038 PM 1793221 ER PT J AU CABIB, E AF CABIB, E TI DIFFERENTIAL INHIBITION OF CHITIN SYNTHETASE-1 AND SYNTHETASE-2 FROM SACCHAROMYCES-CEREVISIAE BY POLYOXIN-D AND NIKKOMYCINS SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID SEPTUM FORMATION; CELL-WALL; ENZYME; YEAST; PURIFICATION; SYNTHASE-1 AB Polyoxin D, nikkomycin X, and nikkomycin Z are all competitive inhibitors of chitin synthetase 2 (Chs2), the essential enzyme for primary septum formation in Saccharomyces cerevisiae, and of Chs1, a repair enzyme. However, Chs2 is more resistant to these antibiotics than Chs1. When Co2+, the best stimulator of Chs2, was used in the assay for this enzyme, the differences in the K(i) values for nikkomycins between the two isozymes reached 3 orders of magnitude. These results point to differences in the active sites of the two isozymes. Polyoxin D was much more effective than nikkomycin Z in inhibiting cell growth. This underlines the importance of the choice of enzyme and of assay conditions when cell wall-synthesizing enzymes are used in screens for possible antifungal agents. RP CABIB, E (reprint author), NIDDKD,BIOCHEM & METAB LAB,BETHESDA,MD 20892, USA. NR 21 TC 101 Z9 108 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD JAN PY 1991 VL 35 IS 1 BP 170 EP 173 PG 4 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA EQ538 UT WOS:A1991EQ53800030 PM 2014972 ER PT J AU SZEBENI, J PATEL, SS HUNG, K WAHL, LM WEINSTEIN, JN AF SZEBENI, J PATEL, SS HUNG, K WAHL, LM WEINSTEIN, JN TI EFFECTS OF THYMIDINE AND URIDINE ON THE PHOSPHORYLATION OF 3'-AZIDO-3'-DEOXYTHYMIDINE (ZIDOVUDINE) IN HUMAN MONONUCLEAR-CELLS SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Note ID CENTRIFUGAL ELUTRIATION CCE; MONOCYTE-ENRICHED FRACTIONS; ANTIRETROVIRAL ACTIVITY; VIRUS INVITRO; MACROPHAGES; TOXICITY; SUBSETS AB The effects of thymidine and uridine on the phosphorylation of 3'-azido-3'-deoxythymidine (AZT) were studied in various human mononuclear cell preparations. Thymidine suppressed ]H-3[AZT phosphorylation in the same concentration range (20 to 100-mu-M) in which it antagonizes the anti-human immunodeficiency virus activity of AZT. Uridine, in turn, had no influence on AZT phosphorylation, just as it has no effect on the anti-human immunodeficiency virus activity of AZT. These findings are consistent with a close relationship between the inhibition of AZT phosphorylation and the influence of physiological nucleosides on the antiviral activity of AZT. C1 NIDR,BETHESDA,MD 20892. RP SZEBENI, J (reprint author), NCI,BETHESDA,MD 20892, USA. NR 17 TC 15 Z9 16 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD JAN PY 1991 VL 35 IS 1 BP 198 EP 200 PG 3 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA EQ538 UT WOS:A1991EQ53800039 PM 2014977 ER PT J AU MCLELLAN, CA DOUDET, DJ BRUCKE, T AIGNER, TG COHEN, RM AF MCLELLAN, CA DOUDET, DJ BRUCKE, T AIGNER, TG COHEN, RM TI NEW RAPID ANALYSIS METHOD DEMONSTRATES DIFFERENCES IN 6-(F-18)FLUORO-L-DOPA PLASMA INPUT CURVES WITH AND WITHOUT CARBIDOPA AND IN HEMI-MPTP LESIONED MONKEYS SO APPLIED RADIATION AND ISOTOPES LA English DT Article ID POSITRON EMISSION TOMOGRAPHY; PERFORMANCE LIQUID-CHROMATOGRAPHY; PARKINSONS-DISEASE; DOPAMINE; METABOLISM; 6-FLUORO-L-DOPA; INHIBITION; BRAIN AB Kinetic modeling of the PET tracer 6-[F-18]fluoro-L-dopa ([F-18]Dopa), used to measure presynaptic dopamine function, requires the accurate determination of the plasma input curve. We have developed a new method that uses alumina extraction preceded by cation and anion exchange resins to determine the parent compound, [F-18]Dopa and its critical metabolite 3-O-methyl-6-[F-18]fluoro-L-dopa. Using this method we found that carbidopa increases the plasma input of [F-18]Dopa while decreasing the rate of metabolite formation, and that previous drug treatment can significantly effect [F-18]Dopa metabolism. C1 NIMH,CEREBRAL METAB SECT CLIN BRAIN IMAGING LAB,BETHESDA,MD 20892. NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. UNIV VIENNA,NEUROL CLIN,A-1090 VIENNA,AUSTRIA. RP MCLELLAN, CA (reprint author), NIMH,CELL BIOL LAB,BLDG 36-3A17,900 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 21 TC 26 Z9 26 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0969-8043 J9 APPL RADIAT ISOTOPES JI Appl. Radiat. Isot. PY 1991 VL 42 IS 9 BP 847 EP 854 PG 8 WC Chemistry, Inorganic & Nuclear; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Chemistry; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA GF251 UT WOS:A1991GF25100013 ER PT J AU ROSENBERG, PS GAIL, MH AF ROSENBERG, PS GAIL, MH TI BACKCALCULATION OF FLEXIBLE LINEAR-MODELS OF THE HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION CURVE SO APPLIED STATISTICS-JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES C LA English DT Article DE HUMAN IMMUNODEFICIENCY VIRUS; MULTINOMIAL DISTRIBUTION; POISSON REGRESSION; PREVALENCE OF INFECTION; PROJECTING ACQUIRED IMMUNE DEFICIENCY SYNDROME CASES; QUASI-LIKELIHOOD ID AIDS EPIDEMIC; UNITED-STATES; HIV INFECTION; SIZE AB The method of backcalculation can be used both to estimate the number of persons infected with the human immunodeficiency virus (HIV) and to project future acquired immune deficiency syndrome (AIDS) incidence. We present a regression approach to backcalculation of flexible linear models of the HIV infection curve. These models include step functions and splines. Because expected AIDS incidence can be expressed as a linear function of unknown parameters, regression methods may be used to obtain parameter and covariance estimates for a variety of interesting quantities, such as the expected number of people infected in previous time intervals and the projected AIDS incidence in future time intervals. We exploit these ideas to show that estimates based on maximum likelihood are, for practical purposes, equivalent to approximate estimates based on quasi-likelihood and on Poisson regression. These algorithms are readily implemented on a personal computer. We illustrate by projecting AIDS incidence in the USA. RP ROSENBERG, PS (reprint author), NCI,BIOSTAT BRANCH,EXECUT PLAZA N,ROOM 403,6130 EXECUT BLVD,ROCKVILLE,MD 20892, USA. NR 21 TC 52 Z9 52 U1 0 U2 0 PU BLACKWELL PUBL LTD PI OXFORD PA 108 COWLEY RD, OXFORD, OXON, ENGLAND OX4 1JF SN 0035-9254 J9 APPL STAT-J ROY ST C JI Appl. Stat.-J. R. Stat. Soc. PY 1991 VL 40 IS 2 BP 269 EP 282 DI 10.2307/2347592 PG 14 WC Statistics & Probability SC Mathematics GA FC186 UT WOS:A1991FC18600004 ER PT J AU PABUCCUOGLU, V ROZWADOWSKA, MD BROSSI, A CLARK, A HUFFORD, CD GEORGE, C FLIPPENANDERSON, JL AF PABUCCUOGLU, V ROZWADOWSKA, MD BROSSI, A CLARK, A HUFFORD, CD GEORGE, C FLIPPENANDERSON, JL TI OXOAPORPHINE ALKALOIDS - CONVERSION OF LYSICAMINE INTO LIRIODENDRONINE AND ITS 2-O-METHYL ETHER, AND ANTIFUNGAL ACTIVITY SO ARCHIV DER PHARMAZIE LA English DT Article ID CONSTITUENTS AB Pschorr reaction of diazonium salt 7 in aqueous methanolic sulfuric acid afforded, besides lysicamine 2, the orange colored sulfate of oxodibenzopyrrocoline (8). The structure is fully supported by an X-ray analysis of its picrate salt. Selective ether cleavage of lysicamine (2) with 48% HBr afforded a hydrobromide of 9, and free betaine 9 on treatment with pyridine-water. Both compounds methylated on treatment with etherial diazomethane on nitrogen to give the known 2-O,N-dimethylliriodendronine (11). Liriodendronine (10) was obtained from lysicamine (2) on heating with pyridine.HBr at 189-degrees-C, and treatment with pyridine-water, as a dark violet betaine. Betaine 12 was obtained by heating 11.HCl to 200-degrees-C. The quatemary salts of lysicamine, lysicamine methiodide (3) and lysicamine methosulfate (4) were comparable in anticandidal activity to liriodenine (1), but were not as active as liriodenine methiodide (13). C1 UNIV MISSISSIPPI,SCH PHARM,DEPT PHARMACOGNOSY,UNIVERSITY,MS 38677. USN,RES LAB,STRUCT MATTER LAB,WASHINGTON,DC 20375. RP PABUCCUOGLU, V (reprint author), NIDDK,LAC,MED CHEM SECT,BETHESDA,MD 20892, USA. NR 18 TC 10 Z9 10 U1 0 U2 2 PU VCH PUBLISHERS INC PI DEERFIELD BEACH PA 303 NW 12TH AVE, DEERFIELD BEACH, FL 33442-1788 SN 0365-6233 J9 ARCH PHARM JI Arch. Pharm. PD JAN PY 1991 VL 324 IS 1 BP 29 EP 33 DI 10.1002/ardp.19913240109 PG 5 WC Chemistry, Medicinal; Chemistry, Multidisciplinary; Pharmacology & Pharmacy SC Pharmacology & Pharmacy; Chemistry GA EW623 UT WOS:A1991EW62300007 PM 2043039 ER PT J AU KANZAKI, H MCPHIE, P MILES, EW AF KANZAKI, H MCPHIE, P MILES, EW TI EFFECT OF SINGLE AMINO-ACID SUBSTITUTIONS AT POSITION-49 AND POSITION-60 ON THE THERMAL UNFOLDING OF THE TRYPTOPHAN SYNTHASE-ALPHA SUBUNIT FROM SALMONELLA-TYPHIMURIUM SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article ID 2 MUTANT PROTEINS; ALPHA-SUBUNIT; ESCHERICHIA-COLI; BETA-SUBUNIT; CONFORMATIONAL STABILITY; CATALYTIC RESIDUE; UNIQUE POSITION; WILD-TYPE; FRAGMENTS; SITE C1 NIDDKD,BIOCHEM PHARMACOL LAB,BLDG 8,ROOM 2A-09,BETHESDA,MD 20892. NIDDKD,BIOCHEM & METAB LAB,BETHESDA,MD 20892. NR 44 TC 14 Z9 14 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD JAN PY 1991 VL 284 IS 1 BP 174 EP 180 DI 10.1016/0003-9861(91)90280-V PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA ER531 UT WOS:A1991ER53100028 PM 1989494 ER PT J AU RAO, PV ZIGLER, JS AF RAO, PV ZIGLER, JS TI ZETA-CRYSTALLIN FROM GUINEA-PIG LENS IS CAPABLE OF FUNCTIONING CATALYTICALLY AS AN OXIDOREDUCTASE SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article ID PROTEIN; DEHYDROGENASES C1 NEI,MECH OCULAR DIS LAB,BLDG 6,ROOM 237,BETHESDA,MD 20892. NR 12 TC 26 Z9 26 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD JAN PY 1991 VL 284 IS 1 BP 181 EP 185 DI 10.1016/0003-9861(91)90281-M PG 5 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA ER531 UT WOS:A1991ER53100029 PM 1989495 ER PT J AU THEWES, M STADLER, R KORGE, B MISCHKE, D AF THEWES, M STADLER, R KORGE, B MISCHKE, D TI NORMAL PSORIATIC EPIDERMIS EXPRESSION OF HYPERPROLIFERATION-ASSOCIATED KERATINS SO ARCHIVES OF DERMATOLOGICAL RESEARCH LA English DT Article DE PSORIASIS; KERATIN EXPRESSION; HYPERPROLIFERATION; KOBNER EFFECT ID MONOCLONAL-ANTIBODY ANALYSIS; CULTURED HUMAN KERATINOCYTES; SQUAMOUS-CELL CARCINOMAS; INTERMEDIATE FILAMENTS; TERMINAL DIFFERENTIATION; VITAMIN-A; EPITHELIAL-CELLS; GENE-EXPRESSION; RETINOIDS; KERATINIZATION AB Keratin expression in lesional, marginal and uninvolved psoriatic epidermis was analysed by one- and two-dimensional gel electrophoresis and immunoblotting. Keratins K1, K5, K6, K1O, K14, and K16 were identified in lesional epidermis. Keratins K6 and K16 were found in all epidermis probes of uninvolved skin, but never occurred in normal epidermis of control skin samples. By means of laser-densitometric evaluation of one-dimensional gels a downregulation of K1 and K1O and an upregulation of K6 and K16 was found in psoriatic epidermis. Unexpectedly, the level of K5 was considerably lower and the level of K14 considerably higher in lesional skin than in normal epidermis. These results demonstrate that not only basal keratinocytes in lesional epidermis but also suprabasal keratinocytes in uninvolved psoriatic epidermis express an altered differentiation pattern. The latter phenomenon could be very important in understanding the development of the so-called "Kobner effect" in psoriatic epidermis. C1 MED CTR MINDEN, DEPT DERMATOL, PORTASTR 7-9, W-4950 MINDEN, GERMANY. FREE UNIV BERLIN, W-1000 BERLIN 33, GERMANY. NIAMSD, DEPT DERMATOL, SKIN BIOL LAB, BETHESDA, MD USA. UNIV CTR CHARLOTTENBURG, INST EXPTL ONCOL & TRANSPLANTAT, BERLIN, GERMANY. NR 42 TC 53 Z9 57 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0340-3696 EI 1432-069X J9 ARCH DERMATOL RES JI Arch. Dermatol. Res. PY 1991 VL 283 IS 7 BP 465 EP 471 DI 10.1007/BF00371784 PG 7 WC Dermatology SC Dermatology GA GK748 UT WOS:A1991GK74800008 PM 1724897 ER PT J AU CLARK, LC CANTOR, KP ALLAWAY, WH AF CLARK, LC CANTOR, KP ALLAWAY, WH TI SELENIUM IN FORAGE CROPS AND CANCER MORTALITY IN UNITED-STATES COUNTIES SO ARCHIVES OF ENVIRONMENTAL HEALTH LA English DT Article ID DIETARY SELENIUM AB The potential protective effect of selenium status on the risk of developing cancer has been examined in animal and epidermiologic studies. This ecological study investigated the association between U.S. county forage selenium status and site- and sex-specific county cancer mortality rates (1950-1969) using weighted least squares regression. Consistent, significant (p < .01) inverse associations were observed for cancers of the lung, rectum, bladder, esophagus, and cervix in a model limited to rural counties and for cancers of the lung, breast, rectum, bladder, esophagus, and corpus uteri in a model of all counties. No consistent significant positive associations were observed in the rural county models. This remarkable degree of consistency for the inverse associations strengths the likelihood of a causal relationship between low selenium status and an increased risk of cancer mortality. C1 NCI,BETHESDA,MD 20892. USDA ARS,ITHACA,NY 14853. RP CLARK, LC (reprint author), UNIV ARIZONA,COLL MED,EPIDEMIOL & BIOMETRY PROGRAM,2504 E ELM ST,TUCSON,AZ 85716, USA. NR 15 TC 161 Z9 170 U1 1 U2 6 PU HELDREF PUBLICATIONS PI WASHINGTON PA 1319 EIGHTEENTH ST NW, WASHINGTON, DC 20036-1802 SN 0003-9896 J9 ARCH ENVIRON HEALTH JI Arch. Environ. Health PD JAN-FEB PY 1991 VL 46 IS 1 BP 37 EP 42 PG 6 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA EY092 UT WOS:A1991EY09200006 PM 1992931 ER PT J AU PICKENS, RW SVIKIS, DS MCGUE, M LYKKEN, DT HESTON, LL CLAYTON, PJ AF PICKENS, RW SVIKIS, DS MCGUE, M LYKKEN, DT HESTON, LL CLAYTON, PJ TI HETEROGENEITY IN THE INHERITANCE OF ALCOHOLISM - A STUDY OF MALE AND FEMALE TWINS SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID CROSS-FOSTERING ANALYSIS; ANTISOCIAL PERSONALITY; FAMILIAL TRANSMISSION; DIAGNOSTIC CRITERIA; ABUSE; CHILDREN; ZYGOSITY; HISTORY AB Genetic influenza on risk for alcoholism was examined in a US treatment sample of 50 monozygotic (MZ) and 64 dizygotic (DZ) male and 31 MZ and 24 DZ female same-sex twin pairs. For the DSM-III composite diagnosis of Alcohol Abuse and/or Dependence, statistically significant MZ/DZ differences in concordance were found with male, but not female, twins. For specific diagnoses, MZ/DZ differences were found in male subjects for both Alcohol Abuse and Alcohol Dependence, while MZ/DZ differences in female subjects were found only for Alcohol Dependence. The male MZ/DZ concordance difference for composite diagnosis but not for Alcohol Dependence could be accounted for statistically by differences in age of onset between MZ and DZ probands. As with alcohol, differences in MZ/DZ concordance were found for DSM-III composite diagnoses of Other Substance Abuse and/or Dependence with male, but not female twins. Using Epidemiological Catchment Area data to estimate the population base rates of both alcohol and other substances use disorders allowed for heritability analyses that showed genetic factors to have only a modest influence on overall risk in both sexes (heritability estimates of approximately 0.35 for male subjects and 0.24 for female subjects). However, evidence for heterogeneity in the pattern of inheritance was also found, suggesting forms of alcoholism that may be moderately to highly heritable. C1 JOHNS HOPKINS UNIV,DEPT PSYCHIAT & BEHAV SCI,BALTIMORE,MD 21218. UNIV MINNESOTA,DEPT PSYCHOL,MINNEAPOLIS,MN 55455. UNIV MINNESOTA,DEPT PSYCHIAT,MINNEAPOLIS,MN 55455. RP PICKENS, RW (reprint author), NIDA,ADDICT RES CTR,4940 EASTERN AVE,BLDG C,ROOM 392,BALTIMORE,MD 21224, USA. FU NIA NIH HHS [AG06886]; NIAAA NIH HHS [AA06500]; NIDA NIH HHS [DA05147] NR 56 TC 387 Z9 390 U1 4 U2 17 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD JAN PY 1991 VL 48 IS 1 BP 19 EP 28 PG 10 WC Psychiatry SC Psychiatry GA ET378 UT WOS:A1991ET37800001 PM 1984758 ER PT J AU HUGHES, JR GUST, SW SKOOG, K KEENAN, RM FENWICK, JW AF HUGHES, JR GUST, SW SKOOG, K KEENAN, RM FENWICK, JW TI SYMPTOMS OF TOBACCO WITHDRAWAL - A REPLICATION AND EXTENSION SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID SMOKING CESSATION; NICOTINE GUM; PHYSICAL-DEPENDENCE; WEIGHT-GAIN; REPLACEMENT; VALIDITY; BEHAVIOR AB Smokers (n = 315) who wished to quit were randomly assigned in a double-blind manner to groups using either nicotine of placebo gum. Self-reported and observed symptoms of tobacco withdrawal were collected before cessation and at follow-ups of 1 to 2 weeks, 1 month, and 6 months. Self-reported and/or observed anger, anxiety, craving, difficulty concentrating, hunger, impatience, and restlessness were the most prominent symptoms of tobacco withdrawal. These symptoms had returned to precessation levels by 1 month except increased weight, hunger, and craving continued for 6 months in many smokers. Nicotine gum decreased most symptoms, including craving and hunger but not weight. Abstinent smokers with more intense withdrawal were not more likely to relapse. Abstinent smokers who gained more weight were less likely to relapse. C1 UNIV VERMONT,DEPT PSYCHOL,BURLINGTON,VT 05405. UNIV VERMONT,DEPT FAMILY PRACTICE,BURLINGTON,VT 05405. UNIV VERMONT,DEPT MED BIOSTAT,BURLINGTON,VT 05405. NIDA,BETHESDA,MD. UNIV MINNESOTA,DEPT PSYCHIAT,MINNEAPOLIS,MN 55455. UNIV MINNESOTA,DEPT PSYCHOL,MINNEAPOLIS,MN 55455. RP HUGHES, JR (reprint author), UNIV VERMONT,COLL MED,DEPT PSYCHIAT,BURLINGTON,VT 05405, USA. FU NIDA NIH HHS [DA-0298, DA-03728, DA-04066] NR 51 TC 328 Z9 332 U1 0 U2 6 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD JAN PY 1991 VL 48 IS 1 BP 52 EP 59 PG 8 WC Psychiatry SC Psychiatry GA ET378 UT WOS:A1991ET37800006 PM 1984762 ER PT J AU WEINBERGER, DR SUDDATH, RL CASANOVA, MF TORREY, EF KLEINMAN, JE AF WEINBERGER, DR SUDDATH, RL CASANOVA, MF TORREY, EF KLEINMAN, JE TI CROWS LATERALIZATION HYPOTHESIS FOR SCHIZOPHRENIA SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Letter ID POSTMORTEM RP WEINBERGER, DR (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,INTRAMURAL RES PROGRAM,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032, USA. NR 4 TC 16 Z9 16 U1 1 U2 3 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD JAN PY 1991 VL 48 IS 1 BP 85 EP 85 PG 1 WC Psychiatry SC Psychiatry GA ET378 UT WOS:A1991ET37800011 PM 1984765 ER PT J AU PRELL, GD KHANDELWAL, JK BURNS, RS LEWITT, PA GREEN, JP AF PRELL, GD KHANDELWAL, JK BURNS, RS LEWITT, PA GREEN, JP TI INFLUENCE OF AGE AND GENDER ON THE LEVELS OF HISTAMINE METABOLITES AND PROS-METHYLIMIDAZOLEACETIC ACID IN HUMAN CEREBROSPINAL-FLUID SO ARCHIVES OF GERONTOLOGY AND GERIATRICS LA English DT Article DE HISTAMINE; TELE-METHYLHISTAMINE; TELE-METHYLIMIDAZOLEACETIC ACID; PROS-METHYLIMIDAZOLEACETIC ACID; LUMBAR CEREBROSPINAL FLUID; AGING; NEUROTRANSMITTER METABOLITES; GENDER ID BRAIN HISTAMINE; RAT-BRAIN; RHESUS-MONKEY; CONCENTRATION GRADIENTS; REGIONAL DISTRIBUTION; TELE-METHYLHISTAMINE; N-METHYLTRANSFERASE; AMINO-ACIDS; LOCALIZATION; HISTIDINE AB The metabolites of histamine, tele-methylhistamine (t-MH) and tele-methylimidazoleacetic acid (t-MIAA), were measured in cerebrospinal fluid (CSF) from 47 subjects with neurological disorders and healthy controls. In lumbar CSF, concentrations of these metabolites were significantly correlated. Levels of t-MH, t-MIAA and their sum (which represents virtually all histamine metabolized in brain) were significantly higher in CSF from older subjects and were positively correlated with age. Females had higher levels of histamine metabolites than males. Males had higher levels of pros-methylimidazoleacetic acid (p-MIAA), an isomer of t-MIAA that is not a metabolite of histamine. Levels of p-MIAA increased with age among men. Analysis of covariance indicated that the subjects' health status had little or no effect on age- or sex-related differences in levels of analytes in CSF; sex-related differences were independent of changes attributed to age. These results are in contrast to those of age-related effects on levels of other aminergic transmitter metabolites in CSF and suggest that metabolic activity of histamine in brain may increase with age. C1 NIMH,EXPTL THERAPEUT SECT,BETHESDA,MD 20892. NINCDS,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. SINAI HOSP DETROIT,CLIN NEUROSCI PROGRAM,DETROIT,MI. SINAI HOSP DETROIT,CTR CELL BIOL,DETROIT,MI. RP PRELL, GD (reprint author), CUNY MT SINAI SCH MED,DEPT PHARMACOL,BOX 1215,NEW YORK,NY 10029, USA. NR 55 TC 16 Z9 16 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0167-4943 J9 ARCH GERONTOL GERIAT JI Arch. Gerontol. Geriatr. PD JAN-FEB PY 1991 VL 12 IS 1 BP 1 EP 12 DI 10.1016/0167-4943(91)90002-8 PG 12 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA EZ959 UT WOS:A1991EZ95900001 PM 15374459 ER PT J AU KAPLAN, MS PRATLEY, R HAWKINS, WJ AF KAPLAN, MS PRATLEY, R HAWKINS, WJ TI SILENT-MYOCARDIAL-ISCHEMIA DURING REHABILITATION FOR CEREBROVASCULAR-DISEASE SO ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION LA English DT Note DE CEREBROVASCULAR DISORDERS; MONITORING; PHYSIOLOGICAL; MYOCARDIAL DISEASES; REHABILITATION ID CORONARY-ARTERY DISEASE; ATTACKS AB In asymptomatic patients the importance of silent ischemic ST-T wave changes on Holter monitoring is known to be a significant predictive variable for one-year mortality of postmyocardial infarction patients. This case report represents the uses of ambulatory ECG to detect ischemic ST changes in patients who have had recent strokes. The cases reported here of silent myocardiac ischemia in stroke patients reflect previous reports in which 70% of the ischemic episodes in patients with symptomatic coronary artery disease are not associated with angina and in which approximately 10% to 15% of acute myocardial infarctions are silent. We now believe that the incidence of "silent" ischemia may be precipitated in poststroke patients during their rehabilitation program. This belief is supported by two main factors. First, a high level of personally relevant mental stress exists which activates the sympathoadrenal system, which may lead to myocardial ischemia. Second, some stroke patients become aphasic and are unable to communicate adequately even if they experience angina symptoms. We have found that poststroke, most patients could not undergo exercise treadmill testing secondary to a variety of factors: inability to coordinate limbs, poor endurance, inability to follow directions, and/or lack of attention. We now propose that 24-hour monitoring for ST-T wave changes poststroke should be considered as part of a vigorous investigation for myocardial ischemia during the rehabilitation of these patients because they have an increased risk of cardiac morbidity. C1 NIA, CLIN PHYSIOL LAB, BALTIMORE, MD 21224 USA. JOHNS HOPKINS MED CTR, BALTIMORE, MD USA. SINAI HOSP, DEPT REHABIL MED, BALTIMORE, MD 21215 USA. NR 10 TC 4 Z9 4 U1 1 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0003-9993 J9 ARCH PHYS MED REHAB JI Arch. Phys. Med. Rehabil. PD JAN PY 1991 VL 72 IS 1 BP 59 EP 62 PG 4 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA ET653 UT WOS:A1991ET65300014 PM 1985625 ER PT J AU NAKAJIMA, T ELOVAARA, E PARK, SS GELBOIN, HV VAINIO, H AF NAKAJIMA, T ELOVAARA, E PARK, SS GELBOIN, HV VAINIO, H TI IMMUNOCHEMICAL DETECTION OF CYTOCHROME-P450 ISOZYMES INDUCED IN RAT-LIVER BY N-HEXANE, 2-HEXANONE AND ACETONYL ACETONE SO ARCHIVES OF TOXICOLOGY LA English DT Article DE N-HEXANE; ACETONYL ACETONE; PHENOBARBITAL; CYTOCHROME-P450; MONOCLONAL ANTIBODY; BENZENE; TOLUENE; 7-ETHOXYRESORUFIN; 7-PENTOXYRESORUFIN ID CHLOROFORM-INDUCED HEPATOTOXICITY; MONOCLONAL-ANTIBODIES; METABOLIZING ENZYMES; BUTYL KETONE; 3 FORMS; BENZENE; BIOTRANSFORMATION; CYTOCHROMES-P-450; MICROSOMES; PROTEINS AB Cytochrome P450 isozymes induced in rat liver by treatment with n-hexane, 2-hexanone and acetonyl acetone (given intraperitoneally 5 mmol/kg for 4 days) were investigated using enzyme assays (benzene, toluene, 7-ethoxyresorufin and 7-pentoxyresorufin metabolism) and monoclonal antibodies (anti-P450IA1/2, anti-P450IIB1/2, anti-P450IIC11/6, anti-P450IIE1(91) and anti-P450IIE1(98)). n-Hexane treatment enhanced the activities of low-K(m) benzene aromatic hydroxylase and toluene side-chain oxidase, but not 7-ethoxyresorufin O-deethylase or 7-pentoxyresorufin O-depentylase. 2-Hexanone or acetonyl acetone treatment enhanced the activities of low- and high-K(m) benzene aromatic hydroxylases, toluene side-chain oxidase and 7-pentoxyresorufin O-depentylase, but not of 7-ethoxyresorufin O-deethylase. Immunoblot analysis showed that anti-P450IA1/2 did not bind liver microsomal protein from either control and treated rats in the region of cytochrome P450s, whereas with anti-P450IIE1(98) a clear-cut band was seen in liver microsomes from control and treated rats, with intensities in the following order: 2-hexanone = acetonyl acetone greater-than-or-equal-to n-hexane > control > phenobarbital. With anti-P450IIB1/2, a band was detected in microsomes from phenobarbital-treated rats, and to a lesser extent, in microsomes from 2-hexanone- and acetonyl acetone-treated rats. Like the immunoblot analysis, anti-P450IIE1(91) inhibited toluene side-chain hydroxylase activity in all microsomes, except in preparations from phenobarbital-treated rats and anti-P450IIB1 in microsomes from phenobarbital-, 2-hexanone- and acetonyl acetone-treated rats. Anti-P450IIC11/6 also inhibited toluene side-chain hydroxylase activity: the inhibited activity in the five different microsome preparations was as follows: n-hexane = control > acetonyl acetone = 2-hexanone = phenobarbital. These results indicate that n-hexane induces only quantitative alterations in the constitutive cytochrome P450 isozyme (P450IIE1), whereas its metabolites 2-hexanone and acetonyl acetone induce not only quantitative changes in constitutive cytochrome P450 (P450IIE1 and P450IIC11/6) but also a different type of isozyme (P450IIB1/2). C1 INST OCCUPAT HLTH,DEPT IND HYG & TOXICOL,SF-00250 HELSINKI,FINLAND. INT AGCY RES CANC,F-69372 LYONS,FRANCE. NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. RP NAKAJIMA, T (reprint author), SHINSHU UNIV,SCH MED,DEPT HYG,MATSUMOTO,NAGANO 390,JAPAN. NR 31 TC 21 Z9 21 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-5761 J9 ARCH TOXICOL JI Arch. Toxicol. PY 1991 VL 65 IS 7 BP 542 EP 547 DI 10.1007/BF01973714 PG 6 WC Toxicology SC Toxicology GA GE636 UT WOS:A1991GE63600004 PM 1781736 ER PT J AU QIAN, Y SAIF, LJ KAPIKIAN, AZ KANG, SY JIANG, B ISHIMARU, Y YAMASHITA, Y OSETO, M GREEN, KY AF QIAN, Y SAIF, LJ KAPIKIAN, AZ KANG, SY JIANG, B ISHIMARU, Y YAMASHITA, Y OSETO, M GREEN, KY TI COMPARISON OF HUMAN AND PORCINE GROUP-C ROTAVIRUSES BY NORTHERN BLOT HYBRIDIZATION ANALYSIS SO ARCHIVES OF VIROLOGY LA English DT Note ID INVITRO TRANSCRIPTION; PARAROTAVIRUS; OUTBREAK; STRAINS; DIARRHEA; VIRUS AB The genetic relationship between human and porcine Gp C rotaviruses and between Gp C and Gp A or B rotaviruses was examined by Northern blot hybridization. Cross-hybridization studies using radiolabeled ssRNA transcript probes demonstrated that the human and porcine Gp C rotaviruses shared a high degree of nucleotide sequence homology in most of the eleven gene segments; the greatest sequence divergence was observed in gene 7. Neither the human nor the porcine Gp C probe hybridized strongly with gene segments from Gp A reference strains or a Gp B bovine rotavirus. These data indicate that genetically, porcine and human Gp C rotaviruses are closely related, whereas they are quite distinct from Gp A or B suggesting that porcine and human Gp C rotaviruses may have evolved from a common ancestral source. C1 NIAID, INFECT DIS LAB, BETHESDA, MD 20892 USA. OHIO STATE UNIV, OHIO AGR RES & DEV CTR, FOOD ANIM HLTH RES PROGRAM, WOOSTER, OH 44691 USA. ISHIMARU CHILDRENS HOSP, EHIME, JAPAN. EHIME PREFECTURE INST PUBL HLTH, EHIME, JAPAN. NR 28 TC 7 Z9 8 U1 0 U2 0 PU SPRINGER-VERLAG WIEN PI VIENNA PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA SN 0304-8608 J9 ARCH VIROL JI Arch. Virol. PY 1991 VL 118 IS 3-4 BP 269 EP 277 DI 10.1007/BF01314037 PG 9 WC Virology SC Virology GA FW419 UT WOS:A1991FW41900013 PM 1648897 ER PT B AU BREWER, HB SANTAMARINAFOJO, S HOEG, JM AF BREWER, HB SANTAMARINAFOJO, S HOEG, JM BE WEBER, PC LEAF, A TI GENETIC-DEFECTS IN THE HUMAN PLASMA APOLIPOPROTEINS SO ATHEROSCLEROSIS : ITS PATHOGENESIS AND THE ROLE OF CHOLESTEROL SE ATHEROSCLEROSIS REVIEWS LA English DT Proceedings Paper CT INTERNATIONAL WORKSHOP ON ATHEROSCLEROSIS : ITS PATHOGENESIS AND THE ROLE OF CHOLESTEROL CY NOV 27-DEC 02, 1990 CL FARO, PORTUGAL SP BAYER RP BREWER, HB (reprint author), NHLBI,MOLEC DIS BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-868-6 J9 ATHER REV PY 1991 VL 23 BP 51 EP 61 PG 11 WC Medicine, General & Internal; Pathology; Physiology SC General & Internal Medicine; Pathology; Physiology GA BV33X UT WOS:A1991BV33X00006 ER PT J AU BANWELL, MG CAMERON, JM COLLIS, MP CRISP, GT GABLE, RW HAMEL, E LAMBERT, JN MACKAY, MF REUM, ME SCOBLE, JA AF BANWELL, MG CAMERON, JM COLLIS, MP CRISP, GT GABLE, RW HAMEL, E LAMBERT, JN MACKAY, MF REUM, ME SCOBLE, JA TI THE PALLADIUM-MEDIATED CROSS COUPLING OF BROMOTROPOLONES WITH ORGANOSTANNANES OR ARYLBORONIC ACIDS - APPLICATIONS TO THE SYNTHESIS OF NATURAL-PRODUCTS AND NATURAL PRODUCT ANALOGS SO AUSTRALIAN JOURNAL OF CHEMISTRY LA English DT Article ID COLCHICINE ANALOGS; CRYSTAL-STRUCTURE; GAS-CHROMATOGRAPHY; BETA-THUJAPLICIN; DERIVATIVES; TROPOLONES; RESOLUTION; COMPLEXES; TUBULIN AB The bromotropolones (4), (5) and (10) undergo palladium-mediated cross coupling with a wide range of organostannanes to produce alkenyl-, alkyl- and aryl-substituted tropolones. The methodology has been applied to the synthesis of the monoterpenes beta-dolabrin (11), beta-thujaplicin (12), 4-isopropyl-7-methoxytropolone (13) and beta-thujaplicinol (14). Cross coupling of bromotropolones (4), (5) and (10) with various aryltrimethylstannanes or arylboronic acids has permitted the preparation of the bicyclic colchicine analogues (30)-(43) which have been tested for tubulin-binding activity. The X-ray crystal structure of the most active of these systems, compound (38), is reported. C1 UNIV ADELAIDE,DEPT ORGAN CHEM,ADELAIDE,SA 5001,AUSTRALIA. LA TROBE UNIV,DEPT CHEM,BUNDOORA,VIC 3083,AUSTRALIA. NCI,NCT,DTP,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. RP BANWELL, MG (reprint author), UNIV MELBOURNE,DEPT CHEM,PARKVILLE,VIC 3052,AUSTRALIA. RI Scoble, Judith/B-4919-2016; Banwell, Martin/H-8354-2014 OI Scoble, Judith/0000-0002-6652-0717; NR 49 TC 42 Z9 42 U1 0 U2 4 PU C S I R O PUBLICATIONS PI COLLINGWOOD PA 150 OXFORD ST, PO BOX 1139, COLLINGWOOD VICTORIA 3066, AUSTRALIA SN 0004-9425 J9 AUST J CHEM JI Aust. J. Chem. PY 1991 VL 44 IS 5 BP 705 EP 728 PG 24 WC Chemistry, Multidisciplinary SC Chemistry GA FM025 UT WOS:A1991FM02500007 ER PT J AU DEGUCHI, Y AF DEGUCHI, Y TI ENHANCED EXPRESSION OF HEAT-SHOCK PROTEIN GENE IN KIDNEY LYMPHOID-CELLS OF LUPUS-PRONE MICE DURING GROWING PROCESS SO AUTOIMMUNITY LA English DT Article DE SLE; HEAT SHOCK PROTEIN; GENE TRANSCRIPTION; LUPUS-PRONE MICE; KIDNEY DISEASES; LYMPHOID CELLS RP DEGUCHI, Y (reprint author), NIH,10687 WEYMOUTH ST 103,BETHESDA,MD 20814, USA. NR 0 TC 6 Z9 6 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 0891-6934 J9 AUTOIMMUNITY JI Autoimmunity PY 1991 VL 10 IS 1 BP 1 EP 5 DI 10.3109/08916939108997141 PG 5 WC Immunology SC Immunology GA GC318 UT WOS:A1991GC31800001 PM 1742420 ER PT J AU AIGNER, TG WALKER, DL MISHKIN, M AF AIGNER, TG WALKER, DL MISHKIN, M TI COMPARISON OF THE EFFECTS OF SCOPOLAMINE ADMINISTERED BEFORE AND AFTER ACQUISITION IN A TEST OF VISUAL RECOGNITION MEMORY IN MONKEYS SO BEHAVIORAL AND NEURAL BIOLOGY LA English DT Article C1 NIMH,NEUROPSYCHOL LAB,BLDG 9,ROOM 1N 107,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 10 TC 100 Z9 101 U1 1 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0163-1047 J9 BEHAV NEURAL BIOL JI Behav. Neural Biol. PD JAN PY 1991 VL 55 IS 1 BP 61 EP 67 DI 10.1016/0163-1047(91)80127-Z PG 7 WC Behavioral Sciences; Neurosciences; Psychology SC Behavioral Sciences; Neurosciences & Neurology; Psychology GA EN647 UT WOS:A1991EN64700005 PM 1996948 ER PT J AU HOROWITZ, SW AF HOROWITZ, SW TI EMPIRICAL SUPPORT FOR STATEMENT VALIDITY ASSESSMENT SO BEHAVIORAL ASSESSMENT LA English DT Article DE CONTENT ANALYSIS; CHILD WITNESSES; SEXUAL ABUSE ID RELIABILITY; ABUSE AB Statement Validity Assessment (SVA) is a procedure designed to elicit and evaluate statements from children who may have been victims of sexual abuse. The core of Statement Validity Assessment is the Undeutsch Hypothesis as operationalized by Criteria-Based Content Analysis (CBCA). The few laboratory and field studies of CBCA that have been completed are reviewed. These studies suggest that CBCA may be a reliable and accurate assessment procedure. However, many questions and research issues remain unresolved. For example, interjudge reliability is the only facet of reliability that has been tested, and the statistics used for those tests capitalized on chance agreement. Only one validity study has occurred in the field. The laboratory results confirm that relevant characteristics of child sexual abuse are difficult to model. Finally, no study in print has addressed the other components of Statement Validity Assessment; the interview guidelines and the Validity Checklist. Several studies of SVA are in progress and are described here. These studies hold the promise of addressing some but not all of the remaining questions about the reliability, validity, and utility of this technique. Suggestions are tendered for future research including the creation of a judicially-sponsored, national data archive. C1 NICHHD,BETHESDA,MD 20892. NR 33 TC 14 Z9 14 U1 2 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0191-5401 J9 BEHAV ASSESS PY 1991 VL 13 IS 3 BP 293 EP 313 PG 21 WC Psychology SC Psychology GA HK334 UT WOS:A1991HK33400006 ER PT J AU MITCHELL, CL BOINSKI, S VANSCHAIK, CP AF MITCHELL, CL BOINSKI, S VANSCHAIK, CP TI COMPETITIVE REGIMES AND FEMALE BONDING IN 2 SPECIES OF SQUIRREL-MONKEYS (SAIMIRI-OERSTEDI AND S-SCIUREUS) SO BEHAVIORAL ECOLOGY AND SOCIOBIOLOGY LA English DT Article ID WHITE COLOBUS MONKEY; SOCIAL-ORGANIZATION; PRIMATE GROUPS; SYSTEMS; PREDATION; EVOLUTION; PATTERNS; HABITAT; ECOLOGY AB Ecological and behavioral data from long-term field studies of known individuals in two closely related squirrel monkey species (Saimiri oerstedi and S. sciureus) were used to examine hypotheses about the source of variation in female bonding among group-living primates. Social relationships in species which live in cohesive groups are thought to depend on the nature of competition for resources. S. oerstedi and S. sciureus both live in large groups and are subject to intense predation. Direct feeding competition both between and within groups is extremely low in S. oerstedi; in this species female relationships are undifferentiated, no female dominance hierarchy is evident and females disperse from their natal group. S. sciureus also experiences very low levels of between-group competition, but within-group direct competition for resources is frequent; this species demonstrates differentiated female relationships, a female dominance hierarchy, and female philo-pastry. The correlated ecological and social variables found in these two congeners further minimize the minor effects of phylogenetic differences and emphasize the importance of food distribution in determining social characteristics. C1 NIH,CTR ANIM,COMPARAT ETHOL LAB,POB 289,BLDG 112,POOLESVILLE,MD 20837. PRINCETON UNIV,DEPT BIOL,PRINCETON,NJ 08544. DUKE UNIV,DEPT BIOL ANTHROPOL & ANAT,DURHAM,NC 27705. NR 38 TC 130 Z9 134 U1 4 U2 27 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-5443 J9 BEHAV ECOL SOCIOBIOL JI Behav. Ecol. Sociobiol. PY 1991 VL 28 IS 1 BP 55 EP 60 PG 6 WC Behavioral Sciences; Ecology; Zoology SC Behavioral Sciences; Environmental Sciences & Ecology; Zoology GA ET870 UT WOS:A1991ET87000008 ER PT J AU STADTMAN, TC DAVIS, JN CHING, WM ZINONI, F BOCK, A AF STADTMAN, TC DAVIS, JN CHING, WM ZINONI, F BOCK, A TI AMINO-ACID-SEQUENCE ANALYSIS OF ESCHERICHIA-COLI FORMATE DEHYDROGENASE (FDHH) CONFIRMS THAT TGA IN THE GENE ENCODES SELENOCYSTEINE IN THE GENE-PRODUCT SO BIOFACTORS LA English DT Article DE FORMATE DEHYDROGENASE; SELENOCYSTEINE; TGA CODON ID GLUTATHIONE-PEROXIDASE; METHANOCOCCUS-VANNIELII; BETA-GALACTOSIDASE; UGA CODON; IDENTIFICATION; PROTEINS; RESIDUE; VECTORS; MOIETY; SITE AB The formate dehydrogenase (FDH(F)) of Escherichia coli is a selenocysteine-containing protein that occurs as a component of the formate-hydrogen lyase complex. The gene encoding this 80 kd polypeptide contains a TGA codon in the open reading frame. Several indirect lines of evidence showed earlier that the selenocysteine residue in the protein is inserted co-translationally in a TGA (UGA) dependent process. Direct proof that the selenocysteine is present in the polypeptide in the position corresponding to TGA as predicted from the gene sequence was obtained by automated amino acid sequence analysis of a Se-75-containing peptide isolated from the protein. Construction of a fusion gene comprising a small segment of the fdhF gene linked to the lacZ gene as reporter greatly facilitated isolation of the selenocysteine-containing protein. Subsequent cleavage of this isolated gene product with endoproteinase Asp-N gave rise to an easily purified small selenocysteine-containing peptide that was amenable to amino acid sequence analysis. C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. USN,INST MED RES,BETHESDA,MD 20814. UNIV MUNICH,LEHRSTUHL MIKROBIOL,W-8000 MUNICH 19,GERMANY. NR 19 TC 20 Z9 21 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0951-6433 J9 BIOFACTORS JI Biofactors PD JAN PY 1991 VL 3 IS 1 BP 21 EP 27 PG 7 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA FY086 UT WOS:A1991FY08600003 PM 1829362 ER PT J AU OPTICAN, LM GAWNE, TJ RICHMOND, BJ JOSEPH, PJ AF OPTICAN, LM GAWNE, TJ RICHMOND, BJ JOSEPH, PJ TI UNIBIASED MEASURES OF TRANSMITTED INFORMATION AND CHANNEL CAPACITY FROM MULTIVARIATE NEURONAL DATA SO BIOLOGICAL CYBERNETICS LA English DT Article ID TWO-DIMENSIONAL PATTERNS; INFERIOR TEMPORAL CORTEX; PRIMARY VISUAL-CORTEX; SINGLE UNITS; 2-DIMENSIONAL PATTERNS; THEORETIC ANALYSIS; TRANSMISSION; EFFICIENCY AB Two measures from information theory, transmitted information and channel capacity, can quantifY the ability of neurons to convey stimulus-dependent information. These measures are calculated using probability functions estimated from stimulus-response data. However, these estimates are biased by response quantization, noise, and small sample sizes. Improved estimators are developed in this paper that depend on both an estimate of the sample-size bias and the noise in the data. C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. RP OPTICAN, LM (reprint author), NEI,SENSORIMOTOR RES LAB,NEURAL MODELING SECT,BLDG 10,ROOM 10-C-101,BETHESDA,MD 20892, USA. NR 24 TC 58 Z9 58 U1 0 U2 5 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-1200 J9 BIOL CYBERN JI Biol. Cybern. PY 1991 VL 65 IS 5 BP 305 EP 310 DI 10.1007/BF00216963 PG 6 WC Computer Science, Cybernetics; Neurosciences SC Computer Science; Neurosciences & Neurology GA GF225 UT WOS:A1991GF22500001 PM 1742368 ER PT J AU WEISSMAN, AD CASANOVA, MF KLEINMAN, JE LONDON, ED DESOUZA, EB AF WEISSMAN, AD CASANOVA, MF KLEINMAN, JE LONDON, ED DESOUZA, EB TI SELECTIVE LOSS OF CEREBRAL CORTICAL SIGMA, BUT NOT PCP BINDING-SITES IN SCHIZOPHRENIA SO BIOLOGICAL PSYCHIATRY LA English DT Article ID POTENTIAL ANTIPSYCHOTIC DRUG; DOPAMINE NEURONAL-ACTIVITY; HUMAN FRONTAL-CORTEX; GUINEA-PIG BRAIN; RAT-BRAIN; HIGH-AFFINITY; RECEPTOR AGONIST; PHENCYCLIDINE; POSTMORTEM; PSYCHOSIS AB Drugs such as phencyclidine (PCP) that interact with PCP and sigma binding sites can produce psychotomimetic effects that resemble some symptoms of schizophrenia. Therefore, it has been suggested that PCP and sigma receptors may be important in the clinical manifestations of schizophrenia. Assays of these two binding sites in human postmortem brains showed consistent significant reductions in the density of sigma, but not PCP sites, in schizophrenics as compared with age-matched and postmortem interval-matched normal and suicide controls. Reductions in the density of sigma binding sites in schizophrenia were most prominent in temporal cerebral cortex, and were accompanied by a small increase in affinity for the ligand [H-3]haloperidol. These data provide the first evidence for alterations in sigma binding sites in schizophrenia, and suggest that selective sigma ligands may be useful in the treatment of the disorder. C1 NIMH,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. RP WEISSMAN, AD (reprint author), NIDA,ADDICT RES CTR,NEUROSCI BRANCH,POB 5180,BALTIMORE,MD 21224, USA. NR 57 TC 127 Z9 129 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD JAN 1 PY 1991 VL 29 IS 1 BP 41 EP 54 DI 10.1016/0006-3223(91)90209-5 PG 14 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA EU025 UT WOS:A1991EU02500005 PM 1848113 ER PT J AU LUEDERS, KK AF LUEDERS, KK TI GENOMIC ORGANIZATION AND EXPRESSION OF ENDOGENOUS RETROVIRUS-LIKE ELEMENTS IN CULTURED RODENT CELLS SO BIOLOGICALS LA English DT Review ID A-PARTICLE GENE; NUCLEOTIDE-SEQUENCE; MOUSE; VIRUS; ETN; FAMILY RP LUEDERS, KK (reprint author), NCI,BIOCHEM LAB,BETHESDA,MD 20892, USA. NR 31 TC 9 Z9 9 U1 0 U2 0 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 1045-1056 J9 BIOLOGICALS JI Biologicals PD JAN PY 1991 VL 19 IS 1 BP 1 EP 7 DI 10.1016/1045-1056(91)90017-E PG 7 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Pharmacology & Pharmacy GA FE628 UT WOS:A1991FE62800001 PM 1646616 ER PT B AU SHILOACH, J VANDEWALLE, M KAUFMAN, JB FASS, R AF SHILOACH, J VANDEWALLE, M KAUFMAN, JB FASS, R BE WHITE, MD REUVENY, S SHAFFERMAN, A TI HIGH-DENSITY GROWTH OF MICROORGANISMS FOR PROTEIN-PRODUCTION SO BIOLOGICALS FROM RECOMBINANT MICROORGANISMS AND ANIMAL CELLS: PRODUCTION AND RECOVERY LA English DT Proceedings Paper CT 34TH OHOLO CONF ON NOVEL STRATEGIES IN PRODUCTION AND RECOVERY OF BIOLOGICALS FROM RECOMBINANT MICROORGANISMS AND ANIMAL CELLS CY 1990 CL EILAT, ISRAEL SP AMGEN, BIOTECHNOL GEN, ICI PHARM GRP, INTERPHARM LABS, ISRAEL INST BIOL RES, ISRAEL ACAD SCI & HUMANITIES, JOSEF MEYERHOFF FUND, MERCK SHARP & DOHME, MINIST SCI & TECHNOL ISRAEL, MINIST TOURISM ISRAEL RP SHILOACH, J (reprint author), NIDDK,LCDB,BIOTECHNOL UNIT,BLDG 6,ROOM B1 33,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU V C H PUBLISHERS PI NEW YORK PA NEW YORK BN 3-527-28084-7 PY 1991 BP 33 EP 46 PG 14 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA BV36M UT WOS:A1991BV36M00003 ER PT J AU FONG, D CHAN, MMY HSIEH, WT AF FONG, D CHAN, MMY HSIEH, WT TI GENE-MAPPING OF HUMAN CATHEPSINS AND CYSTATINS SO BIOMEDICA BIOCHIMICA ACTA LA English DT Article; Proceedings Paper CT 8TH CONF ON PROTEOLYSIS ( ICOP MEETING ) CY OCT 14-18, 1990 CL WILDBAD KREUTH, GERMANY SP SONDERFORSCHUNGSBEREICH, CIBA GEIGY, GRUNENTHAL, PLANTORGAN WERK, SANOFI PHARMA, SCHERING, FOND CHEM IND ID INSITU HYBRIDIZATION; CHROMOSOME-11; ASSIGNMENT; PANEL; CST3 AB Chromosomal locations of human cathepsin B, stefin A and kininogen have been determined. Using techniques of somatic cell hybrids and polymerase chain reaction, cathepsin B has been mapped to 8p22, stefin A to 3cen-q21, and kininogen to 3q26-qter. Comparing this data with currently available mapping loci, it can be concluded that many members of cathepsin are dispersed in different chromosomes, whereas members of the cystatin family are restricted to only two human chromosomes, 3 and 20. C1 NIMH,CLIN NEUROGENET BRANCH,BLDG 10,ROOM 3N218,BETHESDA,MD 20892. RP FONG, D (reprint author), RUTGERS STATE UNIV,DEPT BIOL SCI,PISCATAWAY,NJ 08855, USA. FU NCI NIH HHS [CA49359] NR 20 TC 9 Z9 10 U1 0 U2 0 PU AKADEMIE VERLAG GMBH PI BERLIN PA MUHLENSTRASSE 33-34, D-13187 BERLIN, GERMANY SN 0232-766X J9 BIOMED BIOCHIM ACTA PY 1991 VL 50 IS 4-6 BP 595 EP 598 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GN426 UT WOS:A1991GN42600041 PM 1801728 ER PT J AU DEVRIES, CP VANHAEFTEN, TW OPARA, EC VANDERVEEN, EA AF DEVRIES, CP VANHAEFTEN, TW OPARA, EC VANDERVEEN, EA TI INFLUENCE OF INHIBITION OF INSULIN DEGRADATION ON SCATCHARD ANALYSIS IN H35 RAT HEPATOMA-CELLS SO BIOMEDICA BIOCHIMICA ACTA LA English DT Article ID SITE-SITE INTERACTIONS; NEGATIVE COOPERATIVITY; BINDING; RECEPTOR; PURIFICATION; CHLOROQUINE; HEPATOCYTES; BACITRACIN; MODEL AB Inhibition of insulin degradation by the use of chloroquine and bacitracin leads to a straight line in the Scatchard plot after correction for internalized insulin (r = 0.978 +/- 0.011). Analysis according to a model with two groups of heterogeneous binding sites showed a significant reduction in the total number of binding sites (p < 0.01) in the chloroquine-bacitracin treated cells, with a disappearance of the low affinity binding sites and an increase in the assessment of the number of high affinity binding sites (p < 0.002). The assessment of the high affinity association constant showed a decrease in the chloroquine-bacitracin treated cells (p < 0.05). Analysis according to a model with a homogeneous group of binding sites with negative cooperativity also showed a decrease in the assessment of the number of binding sites (p < 0.05) with a significant reduction in the Ke/Kf ratio from 4.5 +/- 1.5 to 1.16 +/- 0.22 (p < 0.002). These results indicate that inhibition of insulin degradation leads to disappearance of negative cooperativity; the data are inconsistent with the two site model. C1 NIH,BETHESDA,MD 20892. RP DEVRIES, CP (reprint author), FREE UNIV AMSTERDAM HOSP,DEPT ENDOCRINOL,AMSTERDAM,NETHERLANDS. NR 22 TC 0 Z9 0 U1 0 U2 1 PU AKADEMIE VERLAG GMBH PI BERLIN PA MUHLENSTRASSE 33-34, D-13187 BERLIN, GERMANY SN 0232-766X J9 BIOMED BIOCHIM ACTA PY 1991 VL 50 IS 8 BP 1027 EP 1032 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GV538 UT WOS:A1991GV53800011 PM 1781774 ER PT J AU TASAKI, I AF TASAKI, I TI BISTABILITY OF THE NERVE MEMBRANE - MECHANICAL AND THERMAL-CHANGES IN NERVE-FIBERS DURING EXCITATION SO BIOMEDICAL RESEARCH-TOKYO LA English DT Article; Proceedings Paper CT 16TH SEIRIKEN CONF FOR THE MEMORY OF SUMUMU HAGIWARA - BASIC NEUROBIOLOGY : HALF A CENTURY AND FUTURE CY DEC 17-19, 1990 CL NATL INST PHYSIOL SCI, OKAZAKI, JAPAN SP NATL INST PHYSIOL SCI, UNIV CALIF LOS ANGELES, TOKYO IYAKUHIN KOGYO KYOKAI, OSAKA IYAKUHIN KYOKAI, KANEHARA ICHIRO MEM FDN, INOUE FDN PROMOT SCI, YAMADA FDN PROMOT SCI, NARISHIGE FDN, NIPPON ZOKI PHARM, OLYMPUS HO NATL INST PHYSIOL SCI ID IMPULSE AB In view of the great advancement in recent studies of bistability, oscillating reactions and traveling waves in chemical systems, a renewed interest is aroused in explaining the process of excitation of nerve fibers on the basis of bistability of the nerve membrane. The results of recent measurements of rapid mechanical and thermal changes in nerve fibers during excitation are in consonance with this explanation. C1 NIMH,CELL BIOL LAB,BETHESDA,MD 20892. NR 7 TC 0 Z9 0 U1 0 U2 0 PU BIOMED RES FOUND PI TOKYO PA KANDA PO BOX 182 CHIYODAKU, TOKYO 101-91, JAPAN SN 0388-6107 J9 BIOMED RES-TOKYO JI Biomed. Res. PY 1991 VL 12 SU 2 BP 19 EP 21 PG 3 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA GX145 UT WOS:A1991GX14500005 ER PT J AU FITZHUGH, AL CHMURNY, GN KLOSE, JR AF FITZHUGH, AL CHMURNY, GN KLOSE, JR TI A NEW AND FACILE SYNTHETIC ROUTE TO N5-FORMYL TETRAHYDROPTEROYLPOLY-L-GLUTAMATES SO BIOORGANIC & MEDICINAL CHEMISTRY LETTERS LA English DT Article AB A simple, wholly chemical method has been found for preparing N5-formyl tetrahydropteroylpoly-L-glutamates. The method involves: (1) forming an alpha-monoester of N5-formyl tetrahydropteroylmono-L-glutamic acid, (2) coupling its "free" gamma-carboxyl end with an L-glutamate polyester (3) deprotection to give the desired product. RP FITZHUGH, AL (reprint author), NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,DYNCORP,MED CHEM SECT,FREDERICK,MD 21702, USA. NR 10 TC 2 Z9 2 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0960-894X J9 BIOORG MED CHEM LETT JI Bioorg. Med. Chem. Lett. PY 1991 VL 1 IS 3 BP 155 EP 158 DI 10.1016/S0960-894X(01)80789-7 PG 4 WC Chemistry, Medicinal; Chemistry, Organic SC Pharmacology & Pharmacy; Chemistry GA GB089 UT WOS:A1991GB08900004 ER PT J AU BURKE, TR LI, ZH BOLEN, JB MARQUEZ, VE AF BURKE, TR LI, ZH BOLEN, JB MARQUEZ, VE TI STRUCTURAL INFLUENCES OF STYRYL-BASED INHIBITORS ON EPIDERMAL GROWTH-FACTOR RECEPTOR AND P56LCK TYROSINE-SPECIFIC PROTEIN-KINASES SO BIOORGANIC & MEDICINAL CHEMISTRY LETTERS LA English DT Article ID ERBSTATIN; TYRPHOSTINS; ANALOGS AB A structure activity study was conducted on two important members of the styryl class of tyrosine-specific protein kinase inhibitors to examine relative roles which the aryl rings and vinyl side chains play in their inhibitory activity. The ability of four analogs (1a-d) to inhibit autophosphorylation of epidermal growth factor receptor (EGFR) and p56lck tyrosine kinases was examined, with results showing that both the pattern of aromatic hydroxylation and the type of side chain functionality can greatly influence both selectivity and potency. C1 NCI,TUMOR VIRUS BIOL LAB,BETHESDA,MD 20892. NCI,MED BRANCH,BETHESDA,MD 20892. RP BURKE, TR (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MED CHEM LAB,BLDG 37,RM 5C06,BETHESDA,MD 20892, USA. RI Burke, Terrence/N-2601-2014 NR 25 TC 8 Z9 9 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0960-894X J9 BIOORG MED CHEM LETT JI Bioorg. Med. Chem. Lett. PY 1991 VL 1 IS 3 BP 165 EP 168 DI 10.1016/S0960-894X(01)80792-7 PG 4 WC Chemistry, Medicinal; Chemistry, Organic SC Pharmacology & Pharmacy; Chemistry GA GB089 UT WOS:A1991GB08900007 ER PT J AU FITZHUGH, AL AF FITZHUGH, AL TI CHLORAMPHENICOL - HIGH DILUTION FT-IR EVIDENCE FOR AN INTRAMOLECULAR HYDROGEN-BOND SO BIOORGANIC & MEDICINAL CHEMISTRY LETTERS LA English DT Article AB FT-IR spectral evidence is presented which indicates that intramolecular hydrogen-bonding occurs in chloramphenicol. RP FITZHUGH, AL (reprint author), PRI DYNCORP,NCI,FREDERICK CANC RES & DEV CTR,MED CHEM SECT,CHEM SYNTH & ANAL LAB,POB B,FREDERICK,MD 21702, USA. NR 11 TC 1 Z9 1 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0960-894X J9 BIOORG MED CHEM LETT JI Bioorg. Med. Chem. Lett. PY 1991 VL 1 IS 5 BP 253 EP 256 DI 10.1016/S0960-894X(01)81037-4 PG 4 WC Chemistry, Medicinal; Chemistry, Organic SC Pharmacology & Pharmacy; Chemistry GA GB091 UT WOS:A1991GB09100005 ER PT J AU HEBEL, D KIRK, KL KINJO, J KOVACS, T LESIAK, K BALZARINI, J DECLERCQ, E TORRENCE, PF AF HEBEL, D KIRK, KL KINJO, J KOVACS, T LESIAK, K BALZARINI, J DECLERCQ, E TORRENCE, PF TI SYNTHESIS OF A DIFLUOROMETHYLENEPHOSPHONATE ANALOG OF AZT-5'-TRIPHOSPHATE AND ITS INHIBITION OF HIV-1 REVERSE-TRANSCRIPTASE SO BIOORGANIC & MEDICINAL CHEMISTRY LETTERS LA English DT Article ID METHANEDIPHOSPHONATE; FLUORINATION; HYDROLYSIS AB Difluoromethylenebisphosphonic acid was prepared by acetyl hypofluorite-mediated fluorination of tetraisopropyl methylenebisphosphonate and ester hydrolysis. Coupling to 3'-azido-3'-deoxythymidine 5'-monophosphate gave the title compound. The difluoromethylenephosphonate was 30-fold less effective than AZT-triphosphate as a competitive inhibitor of HIV-1 reverse transcriptase but 10-fold more effective than the methylenephosphonate analogue. C1 REGA INST,B-3000 LOUVAIN,BELGIUM. RP HEBEL, D (reprint author), NIDDKD,BIOORGAN CHEM & MED CHEM LABS,BETHESDA,MD 20892, USA. NR 13 TC 31 Z9 31 U1 1 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0960-894X J9 BIOORG MED CHEM LETT JI Bioorg. Med. Chem. Lett. PY 1991 VL 1 IS 7 BP 357 EP 360 DI 10.1016/S0960-894X(01)80472-8 PG 4 WC Chemistry, Medicinal; Chemistry, Organic SC Pharmacology & Pharmacy; Chemistry GA GE797 UT WOS:A1991GE79700006 ER PT J AU CHADWICK, RS AF CHADWICK, RS TI PREDICTION OF THE CARDIAC-MUSCLE FORCE-VELOCITY RELATION FROM ITS FORCE-TIME AND FORCE-LENGTH RELATIONS SO BIORHEOLOGY LA English DT Article; Proceedings Paper CT SYMP AT THE 7TH INTERNATIONAL CONGRESS OF BIORHEOLOGY : CARDIAC MUSCLE CELL RHEOLOGY CY JUN 18-23, 1989 CL NANCY, FRANCE DE MODEL; THEORETICAL MODEL; MYOCARDIAL CONTRACTION; CARDIAC MUSCLE RHEOLOGY ID SARCOMERE-LENGTH AB The force-velocity relation for cardiac muscle fibers can be calculated from a proposed constitutive law based on force-time and force-length data. The calculated force-velocity relation agrees quite well with the measured force-velocity relation obtained from a quick release of sarcomere controlled rat cardiac trabeculae. The theory confirms the measured linear relationship between maximal velocity of sarcomere shortening and sarcomere length. The implication is that the force-velocity relation is not an independent property, and therefore need not be explicitly included as a rheological element in the constitutive law. RP CHADWICK, RS (reprint author), NIH,BIOMED ENGN & INSTRUMENTAT BRANCH,DIV RES SERV,MECH ENGN SECT,THEORET BIOMECH GRP,BETHESDA,MD 20892, USA. NR 12 TC 0 Z9 0 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-355X J9 BIORHEOLOGY JI Biorheology PY 1991 VL 28 IS 3-4 BP 171 EP 176 PG 6 WC Biophysics; Engineering, Biomedical; Hematology SC Biophysics; Engineering; Hematology GA GA213 UT WOS:A1991GA21300007 PM 1932709 ER PT J AU DONG, C SKALAK, R SUNG, KLP AF DONG, C SKALAK, R SUNG, KLP TI CYTOPLASMIC RHEOLOGY OF PASSIVE NEUTROPHILS SO BIORHEOLOGY LA English DT Article; Proceedings Paper CT 7TH INTERNATIONAL CONGRESS ON BIORHEOLOGY CY JUN 18-23, 1989 CL NANCY, FRANCE DE NEUTROPHILS; CYTOPLASMIC VISCOSITY; MICROPIPETTE; MATHEMATICAL MODEL ID VISCOSITY AB The rheological properties of leukocytes are important to their effectiveness in the microcirculation. Previous studies based on in vitro data from micropipette experiments suggest that a Maxwell fluid bounded by a cortical shell with persistent tension is a realistic model for non-activated neutrophils in both the rapid and slow deformation phases. However, various viscoelastic coefficients have been obtained depending on the degree of cell deformation. In the present paper it is demonstrated that the cytoplasmic apparent viscosity and elasticity vary continuously, depending on the degree of deformation. These apparent variations are due to the inhomogeneous nature of the neutrophil internal structure. It is shown that the nucleus is much stiffer than the cytoplasm. The composite structure of the cell results in the deformation-dependent properties. C1 UNIV CALIF SAN DIEGO,DEPT APPL MECH & ENGN SCI BIOENGN,LA JOLLA,CA 92093. RP DONG, C (reprint author), NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,13-3W13,BETHESDA,MD 20892, USA. FU NHLBI NIH HHS [HL-43026] NR 8 TC 94 Z9 99 U1 3 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-355X J9 BIORHEOLOGY JI Biorheology PY 1991 VL 28 IS 6 BP 557 EP 567 PG 11 WC Biophysics; Engineering, Biomedical; Hematology SC Biophysics; Engineering; Hematology GA HE694 UT WOS:A1991HE69400005 PM 1818744 ER PT J AU LUNN, G SANSONE, EB AF LUNN, G SANSONE, EB TI DECONTAMINATION OF AQUEOUS-SOLUTIONS OF BIOLOGICAL STAINS SO BIOTECHNIC & HISTOCHEMISTRY LA English DT Article DE DECONTAMINATION; DISPOSAL; SAFETY; MUTAGENICITY ID ETHIDIUM-BROMIDE; MUTAGENICITY; SALMONELLA; ADSORPTION; DYES AB Aqueous solutions of a number of biological stains were completely decontaminated to the limit of detection using Amberlite resins. Amberlite XAD-16 was the most generally applicable resin but Amberlite XAD-2, Amberlite XAD-4, and Amberlite XAD-7 could be used to decontaminate some solutions. Solutions of acridine orange, alcian blue 8GX, alizarin red S, azure A, azure B, Congo red, cresyl violet acetate, crystal violet, eosin B, erythrosin B, ethidium bromide, Janus green B, methylene blue, neutral red, nigrosin, orcein, propidium iodide, rose Bengal, safranine O, toluidine blue O, and trypan blue could be completely decontaminated to the limit of detection and solutions of eosin Y and Giemsa stain were decontaminated to very low levels (< 0.02 ppm) using Amberlite XAD-16. Reaction times varied from 10 min to 18 hr. Up to 500 ml of a 100-mu-g/ml solution could be decontaminated per gram of Amberlite XAD-16. Fourteen of the 23 stains tested were found to be mutagenic to Salmonella typhimurium. None of the completely decontaminated solutions were found to be mutagenic. RP LUNN, G (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ENVIRONM CONTROL & RES PROGRAM,PROGRAM RESOURCES INC,POB B,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74102] NR 17 TC 8 Z9 9 U1 1 U2 4 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1052-0295 J9 BIOTECH HISTOCHEM JI Biotech. Histochem. PY 1991 VL 66 IS 6 BP 307 EP 315 DI 10.3109/10520299109109992 PG 9 WC Biotechnology & Applied Microbiology; Cell Biology SC Biotechnology & Applied Microbiology; Cell Biology GA GU473 UT WOS:A1991GU47300005 PM 1725856 ER PT J AU DEFILIPPES, FM AF DEFILIPPES, FM TI DECONTAMINATING THE POLYMERASE CHAIN-REACTION SO BIOTECHNIQUES LA English DT Note ID DNA AB Template DNA, which was added to a modified polymerase chain reaction mixture to simulate contamination, was inactivated by restriction enzyme digestion. After inactivation of the restriction enzymes, additional template, buffer and Taq polymerase were added to the reaction and the polymerase chain reaction proceeded. Results are presented using several different restriction enzymes with four base recognition sequences. The limitations of the method are discussed to indicate the tactics to be used for other DNA sequences. RP DEFILIPPES, FM (reprint author), NIAID,VIRAL DIS LAB,BETHESDA,MD 20892, USA. NR 3 TC 25 Z9 25 U1 0 U2 1 PU EATON PUBLISHING CO PI NATICK PA 154 E. CENTRAL ST, NATICK, MA 01760 SN 0736-6205 J9 BIOTECHNIQUES JI Biotechniques PD JAN PY 1991 VL 10 IS 1 BP 26 EP & PG 0 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET526 UT WOS:A1991ET52600004 PM 1848438 ER PT J AU JONES, DH HOWARD, BH AF JONES, DH HOWARD, BH TI A RAPID METHOD FOR RECOMBINATION AND SITE-SPECIFIC MUTAGENESIS BY PLACING HOMOLOGOUS ENDS ON DNA USING POLYMERASE CHAIN-REACTION SO BIOTECHNIQUES LA English DT Article ID ENZYMATIC AMPLIFICATION; CROSSOVER LINKERS AB We have developed a novel polymerase chain reaction (PCR) method that permits the rapid generation of site-specific mutants and recombinant DNA constructs with a minimum number of steps and primers. DNA segments are modified by using amplifying primers that add homologous ends to the polymerase chain reaction product(s). These homologous ends undergo recombination in vivo following transformation of recA-E. coli strains used routinely in cloning. In vivo circularization of PCR products containing plasmid sequences with a selective marker permits the rapid cloning of the desired mutant or recombinant. In the mutagenesis protocol, 7 of the 12 clones contained the product of interest, and 6 of these clones had no detected error (50% of the clones without detected errors). In each of several recombination protocols, at least 50% of the clones tested contained the insert of interest without detected errors. C1 NICHHD,BETHESDA,MD 20892. RP JONES, DH (reprint author), UNIV IOWA,COLL MED,DEPT PEDIAT,IOWA CITY,IA 52242, USA. NR 12 TC 100 Z9 101 U1 1 U2 9 PU EATON PUBLISHING CO PI NATICK PA 154 E. CENTRAL ST, NATICK, MA 01760 SN 0736-6205 J9 BIOTECHNIQUES JI Biotechniques PD JAN PY 1991 VL 10 IS 1 BP 62 EP 66 PG 5 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA ET526 UT WOS:A1991ET52600015 PM 2003926 ER PT B AU LEVY, H SALAZAR, A AF LEVY, H SALAZAR, A BE GEBELEIN, CG TI USE OF THE ANTIVIRAL AND IMMUNE MODULATOR, POLY(ICLC), IN THE TREATMENT OF AIDS SO BIOTECHNOLOGY AND POLYMERS LA English DT Proceedings Paper CT SYMP ON BIOTECHNOLOGY AND POLYMERS CY APR 22-23, 1990 CL BOSTON, MA SP AMER CHEM SOC, DIV POLYM MAT SCI & ENGN, PETR RES FUND, SCI TEC SYMP ASSOC, POLYMER MAT DIV, ALLIED SIGNAL, ROHM & HAAS, ANATRACE RP LEVY, H (reprint author), NIAID,OFF SCI DIRECTOR,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA NEW YORK BN 0-306-44049-0 PY 1991 BP 11 EP 29 PG 19 WC Biotechnology & Applied Microbiology; Polymer Science SC Biotechnology & Applied Microbiology; Polymer Science GA BV12M UT WOS:A1991BV12M00002 ER PT B AU LOCKWICH, T SHAMOO, AE AF LOCKWICH, T SHAMOO, AE BE VERNA, R NISHIZUKA, Y TI TERTIARY STRUCTURE OF SARCOPLASMIC-RETICULUM (CA2++MG2+)-ATPASE USING EU3+ LUMINESCENCE TECHNIQUES - IMPLICATIONS ON ENERGY COUPLING SO BIOTECHNOLOGY OF CELL REGULATION SE SERONO SYMPOSIA SERIES : ADVANCES IN EXPERIMENTAL MEDICINE LA English DT Proceedings Paper CT SYMP ON BIOTECHNOLOGY OF CELL REGULATION CY JUN 20-22, 1991 CL SIENA, ITALY SP ARES SERONO SYMP RP LOCKWICH, T (reprint author), NIDR,BLDG 10,RM 1A05,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-788-4 J9 SER SYMP AD PY 1991 VL 4 BP 23 EP 33 PG 11 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA BV61X UT WOS:A1991BV61X00003 ER PT B AU AMBUDKAR, IS LOCKWICH, T HIRAMATSU, Y BAUM, BJ AF AMBUDKAR, IS LOCKWICH, T HIRAMATSU, Y BAUM, BJ BE VERNA, R NISHIZUKA, Y TI REGULATION OF CALCIUM ENTRY IN RAT PAROTID ACINAR-CELLS - POSSIBLE ROLE OF MEMBRANE-POTENTIAL AND CYTOSOLIC CA2+ SO BIOTECHNOLOGY OF CELL REGULATION SE SERONO SYMPOSIA SERIES : ADVANCES IN EXPERIMENTAL MEDICINE LA English DT Proceedings Paper CT SYMP ON BIOTECHNOLOGY OF CELL REGULATION CY JUN 20-22, 1991 CL SIENA, ITALY SP ARES SERONO SYMP RP AMBUDKAR, IS (reprint author), NIDR,CLIN INVEST & PATIENT CARE BRANCH,BLDG 10,ROOM 1N-113,BETHESDA,MD 20892, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-788-4 J9 SER SYMP AD PY 1991 VL 4 BP 35 EP 42 PG 8 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA BV61X UT WOS:A1991BV61X00004 ER PT B AU BLUMENTHAL, R PURI, A DIMITROV, DS AF BLUMENTHAL, R PURI, A DIMITROV, DS BE VERNA, R NISHIZUKA, Y TI INITIAL STEPS OF ENVELOPED VIRUS ENTRY INTO ANIMAL-CELLS SO BIOTECHNOLOGY OF CELL REGULATION SE SERONO SYMPOSIA SERIES : ADVANCES IN EXPERIMENTAL MEDICINE LA English DT Proceedings Paper CT SYMP ON BIOTECHNOLOGY OF CELL REGULATION CY JUN 20-22, 1991 CL SIENA, ITALY SP ARES SERONO SYMP RP BLUMENTHAL, R (reprint author), NCI,LMMB,MEMBRANE STRUCT & FUNCT SECT,BETHESDA,MD 20892, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-788-4 J9 SER SYMP AD PY 1991 VL 4 BP 115 EP 133 PG 19 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA BV61X UT WOS:A1991BV61X00011 ER PT B AU PASTAN, I AF PASTAN, I BE VERNA, R NISHIZUKA, Y TI ONCOTOXINS - NEW THERAPEUTIC AGENTS FOR THE TREATMENT OF CANCER SO BIOTECHNOLOGY OF CELL REGULATION SE SERONO SYMPOSIA SERIES : ADVANCES IN EXPERIMENTAL MEDICINE LA English DT Proceedings Paper CT SYMP ON BIOTECHNOLOGY OF CELL REGULATION CY JUN 20-22, 1991 CL SIENA, ITALY SP ARES SERONO SYMP RP PASTAN, I (reprint author), NCI,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-788-4 J9 SER SYMP AD PY 1991 VL 4 BP 169 EP 174 PG 6 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA BV61X UT WOS:A1991BV61X00014 ER PT B AU MIKI, T FLEMING, T AARONSON, SA AF MIKI, T FLEMING, T AARONSON, SA BE VERNA, R NISHIZUKA, Y TI EXPRESSION CDNA CLONING OF GROWTH-CONTROL GENES SO BIOTECHNOLOGY OF CELL REGULATION SE SERONO SYMPOSIA SERIES : ADVANCES IN EXPERIMENTAL MEDICINE LA English DT Proceedings Paper CT SYMP ON BIOTECHNOLOGY OF CELL REGULATION CY JUN 20-22, 1991 CL SIENA, ITALY SP ARES SERONO SYMP RP MIKI, T (reprint author), NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-788-4 J9 SER SYMP AD PY 1991 VL 4 BP 193 EP 203 PG 11 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA BV61X UT WOS:A1991BV61X00016 ER PT B AU PAPAS, TS MAVROTHALASSITIS, G SETH, A WATSON, DK LAUTENBERGER, JA FISHER, RJ BHAT, NK ASCIONE, R AF PAPAS, TS MAVROTHALASSITIS, G SETH, A WATSON, DK LAUTENBERGER, JA FISHER, RJ BHAT, NK ASCIONE, R BE VERNA, R NISHIZUKA, Y TI THE ETS GENE FAMILY AS A PARADIGM FOR THE STUDY OF CELLULAR TRANSCRIPTIONAL PROCESSES SO BIOTECHNOLOGY OF CELL REGULATION SE SERONO SYMPOSIA SERIES : ADVANCES IN EXPERIMENTAL MEDICINE LA English DT Proceedings Paper CT SYMP ON BIOTECHNOLOGY OF CELL REGULATION CY JUN 20-22, 1991 CL SIENA, ITALY SP ARES SERONO SYMP RP PAPAS, TS (reprint author), NCI,MOLEC ONCOL LAB,FREDERICK,MD 21702, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-788-4 J9 SER SYMP AD PY 1991 VL 4 BP 211 EP 226 PG 16 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA BV61X UT WOS:A1991BV61X00018 ER PT B AU MERLINO, GT MICKISCH, G JHAPPAN, C GALSKI, H TAKAGI, H SMITH, GH FAUSTO, N PASTAN, I GOTTESMAN, MM AF MERLINO, GT MICKISCH, G JHAPPAN, C GALSKI, H TAKAGI, H SMITH, GH FAUSTO, N PASTAN, I GOTTESMAN, MM BE VERNA, R NISHIZUKA, Y TI CELLULAR PROLIFERATION AND DRUG-RESISTANCE IN TRANSGENIC MICE SO BIOTECHNOLOGY OF CELL REGULATION SE SERONO SYMPOSIA SERIES : ADVANCES IN EXPERIMENTAL MEDICINE LA English DT Proceedings Paper CT SYMP ON BIOTECHNOLOGY OF CELL REGULATION CY JUN 20-22, 1991 CL SIENA, ITALY SP ARES SERONO SYMP RP MERLINO, GT (reprint author), NCI,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-788-4 J9 SER SYMP AD PY 1991 VL 4 BP 271 EP 283 PG 13 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA BV61X UT WOS:A1991BV61X00022 ER PT J AU TROUCHE, D ROBIN, P SASSONECORSI, P FARRAR, WL HARELBELLAN, A AF TROUCHE, D ROBIN, P SASSONECORSI, P FARRAR, WL HARELBELLAN, A TI THE DYAD SYMMETRY ELEMENT IS THE MOLECULAR TARGET FOR C-FOS INDUCTION AND INHIBITION DURING K-562 DIFFERENTIATION ALONG MUTUALLY EXCLUSIVE LINEAGES SO BLOOD LA English DT Article ID PROTO-ONCOGENE EXPRESSION; SERUM RESPONSE ELEMENT; PROMYELOCYTIC LEUKEMIA-CELLS; BINDING-SITE; MONOCYTIC DIFFERENTIATION; GENE-EXPRESSION; PHORBOL ESTERS; GROWTH-FACTOR; TRANSCRIPTIONAL ACTIVATION; TERMINAL DIFFERENTIATION C1 INST GUSTAVE ROUSSY,IMMUNOL LAB,CNRS,UA 1156,PR1,39 RUE CAMILLE DESMOULINS,F-94805 VILLEJUIF,FRANCE. INST CHIM BIOL,CNRS,LGME,STRASBOURG,FRANCE. NCI,FREDERICK CANC RES FACIL,MOLEC IMMUNOL LAB,BIOL RESPONSE MODIFIER PROGRAM,FREDERICK,MD 21701. RI Trouche, Didier/B-2854-2008; Sassone-Corsi, Paolo/H-6182-2011; Harel-Bellan, Annick/M-9795-2015; OI Harel-Bellan, Annick/0000-0002-2339-153X NR 78 TC 9 Z9 10 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD JAN 1 PY 1991 VL 77 IS 1 BP 55 EP 63 PG 9 WC Hematology SC Hematology GA EQ041 UT WOS:A1991EQ04100008 PM 1898633 ER PT J AU RODGERS, GP AF RODGERS, GP TI EFFECT OF DEHYDRATION ON THE VISCOELASTIC BEHAVIOR OF RED-CELLS - COMMENTS SO BLOOD CELLS LA English DT Editorial Material ID SICKLE CELLS; DEFORMABILITY; ERYTHROCYTES; FILTRATION; HEMOGLOBIN; BLOOD RP RODGERS, GP (reprint author), NIH,CHEM BIOL LAB,BLDG 10,ROOM 9N-318,BETHESDA,MD 20892, USA. NR 14 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-4684 J9 BLOOD CELLS JI Blood Cells PY 1991 VL 17 IS 3 BP 523 EP 525 PG 3 WC Hematology SC Hematology GA GE451 UT WOS:A1991GE45100008 ER PT J AU FRASER, CM AF FRASER, CM TI MOLECULAR-BIOLOGY OF ADRENERGIC-RECEPTORS - MODEL SYSTEMS FOR THE STUDY OF G-PROTEIN-MEDIATED SIGNAL TRANSDUCTION SO BLOOD VESSELS LA English DT Article; Proceedings Paper CT 7TH INTERNATIONAL SATELLITE SYMP OF THE 11TH INTERNATIONAL CONGRESS OF PHARMACOLOGY : VASCULAR NEUROEFFECTOR MECHANISMS CY JUL 08-11, 1990 CL BONN, FED REP GER DE ALPHA-ADRENERGIC RECEPTORS; BETA-ADRENERGIC RECEPTORS; MUSCARINIC ACETYLCHOLINE RECEPTORS; LIGAND-BINDING SITE; AGONIST ACTIVATION; SITE-DIRECTED MUTAGENESIS; SIGNAL TRANSDUCTION; GENE EXPRESSION; G-PROTEINS; AMINO ACID HOMOLOGY ID SITE-DIRECTED MUTAGENESIS; MUSCARINIC CHOLINERGIC RECEPTORS; SEQUENCE-ANALYSIS; LIGAND-BINDING; ALPHA-2-ADRENERGIC RECEPTOR; ADENYLATE-CYCLASE; AGONIST BINDING; HUMAN-BRAIN; CLONING; EXPRESSION AB Elucidation of the gene structure of several receptors known to mediate the signal of hormone or transmitter binding to intracellular effector systems through guanine-nucleotide-binding proteins (G proteins) has revealed that these receptors comprise a superfamily of related proteins. The hallmark of all G-protein-linked receptors is a presumed topography of 7 membrane-spanning loops, analogous to the structure of bacteriorhodopsin. Members of this gene superfamily contain regions, particularly with the hydrophobic domains, of homologous sequence. The expression of G-protein-linked receptors in heterologous cell systems has allowed for the study of the pharmacological and biochemical properties of individual receptor subtypes in a manner not previously possible with intact tissues containing multiple receptors. Site-directed mutagenesis experiments have identified many conserved amino acids which are involved in ligand binding, receptor activation by agonists and receptor-G protein coupling, and suggest that the conservation of receptor structure throughout this gene family may reflect a conservation of important functional domains within these proteins. RP FRASER, CM (reprint author), NIAAA,PHYSIOL & PHARMACOL STUDIES LAB,MOLEC NEUROBIOL LAB,ROCKVILLE,MD 20852, USA. OI Fraser, Claire/0000-0003-1462-2428 NR 39 TC 10 Z9 10 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0303-6847 J9 BLOOD VESSELS PD JAN-JUN PY 1991 VL 28 IS 1-3 BP 93 EP 103 PG 11 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA FB336 UT WOS:A1991FB33600013 PM 1848129 ER PT J AU ABRAHAM, VS SYKES, M AF ABRAHAM, VS SYKES, M TI MECHANISM OF THE ANTI-GVHD EFFECT OF IL-2 .1. PROTECTIVE HOST-TYPE CELL-POPULATIONS ARE NOT INDUCED BY IL-2 TREATMENT ALONE SO BONE MARROW TRANSPLANTATION LA English DT Article ID DISEASE AB We have recently demonstrated that high-dose IL-2 administered for a short period (2.5 days) beginning on the day of bone marrow transplantation mediates a marked protective effect against GVHD in mice, while preserving the ability to achieve alloengraftment (1). This protective effect is augmented by administration of T cell-depleted (TCD) syngeneic marrow, and is dependent upon early administration of IL-2 (1). The graft-vs-tumor effect against the EL4 leukemia/lymphoma is not diminished in animals protected from GVHD by IL-2 (2). In an attempt to determine whether or not IL-2-activated host-type cells might be responsible for GVHD protection, we have now performed adoptive transfer studies. The results failed to provide evidence that treatment of lethally irradiated mice with IL-2 activates protective host-derived or syngeneic marrow-derived cell populations which can be adoptively transferred to lethally irradiated secondary recipients receiving allogeneic GVHD-producing inocula. Likewise, treatment of lethally irradiated mice with a complete 2.5-day course of IL-2 prior to administration of allogeneic inocula did not lead to GVHD protection. These results suggest that either IL-2 directly inhibits the GVH reactivity of allogeneic GVH-reactive cells, or that GVH reactivity is attenuated by IL-2 during the period of interaction of donor- and host-type cells. RP ABRAHAM, VS (reprint author), NCI,IMMUNOL BRANCH,BLDG 10,ROOM 4B13,BETHESDA,MD 20892, USA. NR 10 TC 6 Z9 6 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0268-3369 J9 BONE MARROW TRANSPL JI Bone Marrow Transplant. PY 1991 VL 7 SU 1 BP 29 EP 32 PG 4 WC Biophysics; Oncology; Hematology; Immunology; Transplantation SC Biophysics; Oncology; Hematology; Immunology; Transplantation GA FH683 UT WOS:A1991FH68300008 PM 2043882 ER PT J AU SANSONE, R DIMARTINO, D LANINO, E DINI, G MASSIMO, L TONINI, GP AF SANSONE, R DIMARTINO, D LANINO, E DINI, G MASSIMO, L TONINI, GP TI MYCN AMPLIFICATION DOES NOT AFFECT SURVIVAL OF NEUROBLASTOMA PATIENTS TREATED WITH AUTOLOGOUS BONE-MARROW TRANSPLANTATION SO BONE MARROW TRANSPLANTATION LA English DT Article; Proceedings Paper CT INTERNATIONAL COURSE ON BONE MARROW TRANSPLANTATION IN CHILDREN CY NOV 28-DEC 01, 1990 CL ERICE, ITALY ID NEURO-BLASTOMA; CATECHOLAMINE METABOLISM; ONCOGENE AMPLIFICATION; MULTIDRUG RESISTANCE; INVERSE CORRELATION; EXPRESSION; GENE; PROGRESSION; CHILDREN; STAGE AB From an extended series of neuroblastoma cases evaluated for MYCN amplification (MNA) at the "G. Gaslini Hospital 15 (4 with and 11 without NMA) underwent myeloablative therapy and bone marrow transplantation (MAT-ABMT). Such cases ranged in age at diagnosis from 13 months to 7 years and were followed up at least 8 months after MAT-ABMT. MNA was present in 2/10 cases dead for disease, in 0/1 cases alive with disease, and in 2/4 cases presently in complete clinical remission. This preliminary evidence would discourage to consider MNA as a marker capable of predicting the final outcome of patients with metastatic Nb. C1 G GASLINI HOSP,DIV PEDIAT HEMATOL ONCOL,GENOA,ITALY. G GASLINI HOSP,MOLEC DIAGNOST RES GRP,GENOA,ITALY. RP SANSONE, R (reprint author), NATL CANC INST,POPUL GENET SECT,GENOA,ITALY. NR 20 TC 2 Z9 2 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0268-3369 J9 BONE MARROW TRANSPL JI Bone Marrow Transplant. PY 1991 VL 7 SU 3 BP 133 EP 135 PG 3 WC Biophysics; Oncology; Hematology; Immunology; Transplantation SC Biophysics; Oncology; Hematology; Immunology; Transplantation GA FR137 UT WOS:A1991FR13700036 PM 1855077 ER PT J AU Webster, HD AF Webster, Henry deF. TI The Growth of Neuropathology in Japan Address of the ISN President at the Opening Ceremony, XI International Congress of Neuropathology SO BRAIN PATHOLOGY LA English DT Editorial Material C1 [Webster, Henry deF.] NIH, Bethesda, MD 20892 USA. RP Webster, HD (reprint author), NIH, Bldg 10, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1015-6305 J9 BRAIN PATHOL JI Brain Pathol. PD JAN PY 1991 VL 1 IS 2 BP 133 EP 134 PG 2 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA V16GY UT WOS:000207859300010 ER PT J AU BERGER, TG SAWCHUK, WS LEONARDI, C LANGENBERG, A TAPPERO, J LEBOIT, PE AF BERGER, TG SAWCHUK, WS LEONARDI, C LANGENBERG, A TAPPERO, J LEBOIT, PE TI EPIDERMODYSPLASIA VERRUCIFORMIS-ASSOCIATED PAPILLOMAVIRUS INFECTION COMPLICATING HUMAN-IMMUNODEFICIENCY-VIRUS DISEASE SO BRITISH JOURNAL OF DERMATOLOGY LA English DT Article ID PATIENT; RECIPIENTS; CARCINOMA; WARTS AB Three males infected with the human immunodeficiency virus (HIV) were noted to have extensive flat warts of the face and/or body. In two there were also pityriasis versicolor-like lesions. Biopsies showed foamy, basophilic, distended cytoplasm in granular layer keratinocytes, characteristic of the human papillomavirus types seen in epidermodysplasia verruciformis. DNA hybridization techniques demonstrated the presence of HPV-type 8 in one patient and HPV 5 and 8 in another. Patients with immune suppression due to HIV infection may demonstrate the clinical features of epidermodysplasia verruciformis with the same potentially oncogenic HPV types. C1 UNIV CALIF SAN FRANCISCO,SCH MED,DEPT PATHOL,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,SCH MED,DEPT DERMATOL,SAN FRANCISCO,CA 94143. NCI,CELLULAR ONCOL LAB,BETHESDA,MD 20892. UNIV MIAMI,DEPT DERMATOL,MIAMI,FL 33152. NR 18 TC 60 Z9 60 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0007-0963 J9 BRIT J DERMATOL JI Br. J. Dermatol. PD JAN PY 1991 VL 124 IS 1 BP 79 EP 83 DI 10.1111/j.1365-2133.1991.tb03286.x PG 5 WC Dermatology SC Dermatology GA ET449 UT WOS:A1991ET44900013 PM 1847068 ER PT J AU DURCAN, MJ WOZNIAK, KM LINNOILA, M AF DURCAN, MJ WOZNIAK, KM LINNOILA, M TI MODULATION OF THE HYPOTHERMIC AND HYPERGLYCEMIC EFFECTS OF 8-OH-DPAT BY ALPHA-2-ADRENOCEPTOR ANTAGONISTS SO BRITISH JOURNAL OF PHARMACOLOGY LA English DT Article ID 8-HYDROXY-2-(DI-N-PROPYLAMINO) TETRALIN 8-OH-DPAT; ADRENOCEPTOR-MEDIATED MODULATION; ALPHA-2 ADRENERGIC-RECEPTORS; BRAIN CORTEX SLICES; INSULIN RELEASE; ALPHA-2-ADRENOCEPTOR ANTAGONIST; SEROTONIN RELEASE; PANCREATIC-ISLETS; RAT ISLETS; BROWN FAT AB 1 The effects of pretreatment with two novel and relatively specific alpha-2-adrenoceptor antagonists on the hypothermic and hyperglycaemic responses induced by the 5-HT1A receptor agonist 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) were investigated in mice. The alpha-2-adrenoceptor antagonists used were, atipamezole, which occupies both central and peripheral receptors, and L 659,066, which poorly penetrates the blood brain barrier. 2 Atipamezole (1 and 3 mg kg-1) alone had no effect on body temperature but significantly attenuated the 8-OH-DPAT-induced hypothermic response. The hyperglycaemic effect of 8-OH-DPAT was also attenuated by pretreatment with atipamezole; however, 3 mg kg-1 atipamezole did cause some hypoglycaemia when administered alone. 3 Pretreatment with L 659,066 (3-30 mg kg-1) failed to alter the hypothermic effects of 8-OH-DPAT. All doses of L 659,066 tested attenuated 8-OH-DPAT-induced hyperglycaemia, but the highest dose (30 mg kg-1) produced hypoglycaemia when administered alone. 4 The results suggest that the attenuation of 8-OH-DPAT-induced hypothermia by alpha-2-adrenoceptor antagonists may be centrally mediated whereas the blockade of 8-OH-DPAT-induced hyperglycaemia may involve peripheral mechanisms. RP DURCAN, MJ (reprint author), NIAAA,DICBR,CLIN STUDIES LAB,BLDG 10 3C102,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 42 TC 27 Z9 28 U1 1 U2 1 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0007-1188 J9 BRIT J PHARMACOL JI Br. J. Pharmacol. PD JAN PY 1991 VL 102 IS 1 BP 222 EP 226 PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA ET129 UT WOS:A1991ET12900037 PM 1675146 ER PT J AU CHRZANOWSKA, M YEH, H ROZWADOWSKA, MD AF CHRZANOWSKA, M YEH, H ROZWADOWSKA, MD TI SYNTHESIS OF SECOISOQUINOLINE ALKALOIDS - TOTAL SYNTHESIS OF ADLUMIDICEINE, ITS ENOL LACTONES AND BICUCULLININE SO BULLETIN OF THE POLISH ACADEMY OF SCIENCES-CHEMISTRY LA English DT Article ID CHEMISTRY; IDENTIFICATION; PAPAVERACEAE; PLANTS AB Four secophthalideisoquinoline alkaloids: adlumidiceine (4), its enol lactones 6 and 7 and bicucullinine (5) have been synthesized from a common intermediate 3. The structure of 3 has been established by spectral data analysis and chemical transformation. C1 NIDDK,ANALYT CHEM LAB,BETHESDA,MD 20892. ADAM MICKIEWICZ UNIV,FAC CHEM,PL-60780 POZNAN,POLAND. RI Chrzanowska, Maria/A-4618-2012 NR 17 TC 2 Z9 2 U1 0 U2 0 PU POLISH SCIENTIFIC PUBL PI WARSAW PA MIODOWA 10, 00-251 WARSAW, POLAND SN 0239-7285 J9 B POL ACAD SCI-CHEM JI Bull. Pol. Acad. Sci.-Chem. PY 1991 VL 39 IS 1 BP 7 EP 12 PG 6 WC Chemistry, Multidisciplinary SC Chemistry GA HM271 UT WOS:A1991HM27100002 ER PT J AU MAVALANKAR, DV TRIVEDI, CR GRAY, RH AF MAVALANKAR, DV TRIVEDI, CR GRAY, RH TI LEVELS AND RISK-FACTORS FOR PERINATAL-MORTALITY IN AHMEDABAD, INDIA SO BULLETIN OF THE WORLD HEALTH ORGANIZATION LA English DT Article ID GESTATIONAL-AGE; BIOMASS FUEL; RURAL INDIA; HEALTH; STILLBIRTHS; BIRTHS; CARE AB To estimate levels and determinants of perinatal mortality, we conducted a hospital-based surveillance and case-control study, linked with a population survey, in Ahmedabad, India. The perinatal mortality rate was 79.0 per 1000, and was highest for preterm low-birth-weight babies. The case-control study of 451 stillbirths, 160 early neonatal deaths and 1465 controls showed that poor maternal nutritional status, absence of antenatal care, and complications during labour were independently associated with substantially increased risks of perinatal death. Multivariate analyses indicate that socioeconomic factors largely operate through these proximate factors and do not have an independent effect. Estimates of attributable risk derived from the prevalence of exposures in the population survey suggest that improvements in maternal nutrition and antenatal and intrapartum care could result in marked reductions of perinatal mortality. C1 NHL MUNICIPAL MED COLL,AHMEDABAD,INDIA. JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,BALTIMORE,MD 21218. RP MAVALANKAR, DV (reprint author), NICHHD,BLDG EPN,ROOM 640,BETHESDA,MD 20892, USA. NR 27 TC 52 Z9 55 U1 0 U2 4 PU WORLD HEALTH ORGANIZATION PI GENEVA 27 PA DISTRIBUTION AND SALES, CH-1211 GENEVA 27, SWITZERLAND SN 0042-9686 J9 B WORLD HEALTH ORGAN JI Bull. World Health Organ. PY 1991 VL 69 IS 4 BP 435 EP 442 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA GJ303 UT WOS:A1991GJ30300008 PM 1934237 ER PT B AU ARMSTRONG, DL ROSSIER, MF SHCHERBATKO, AD WHITE, RE AF ARMSTRONG, DL ROSSIER, MF SHCHERBATKO, AD WHITE, RE BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI ENZYMATIC GATING OF VOLTAGE-ACTIVATED CALCIUM CHANNELS SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP ARMSTRONG, DL (reprint author), NIEHS,CELLULAR & MOLEC PHARMACOL LAB 7-07,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 37 Z9 37 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 26 EP 34 DI 10.1111/j.1749-6632.1991.tb36478.x PG 9 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00004 PM 1660238 ER PT B AU VOGEL, SS DELANEY, K ZIMMERBERG, J AF VOGEL, SS DELANEY, K ZIMMERBERG, J BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI THE SEA-URCHIN CORTICAL REACTION - A MODEL SYSTEM FOR STUDYING THE FINAL STEPS OF CALCIUM-TRIGGERED VESICLE FUSION SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP VOGEL, SS (reprint author), NICHHD,THEORET & PHYS BIOL LAB,BETHESDA,MD 20892, USA. RI Vogel, Steven/A-3585-2012; OI Vogel, Steven/0000-0002-3005-2667 NR 0 TC 26 Z9 26 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 35 EP 44 DI 10.1111/j.1749-6632.1991.tb36479.x PG 10 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00005 PM 1741591 ER PT B AU ETCHEBERRIGARAY, R FIEDLER, JL POLLARD, HB ROJAS, E AF ETCHEBERRIGARAY, R FIEDLER, JL POLLARD, HB ROJAS, E BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI ENDOPLASMIC-RETICULUM AS A SOURCE OF CA-2+ IN NEUROTRANSMITTER SECRETION SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP ETCHEBERRIGARAY, R (reprint author), NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD 20892, USA. RI Fiedler, Jenny/I-5617-2016 NR 0 TC 9 Z9 9 U1 0 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 90 EP 99 DI 10.1111/j.1749-6632.1991.tb36484.x PG 10 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00010 PM 1683762 ER PT B AU BLUMENTHAL, R SCHOCH, C PURI, A CLAGUE, MJ AF BLUMENTHAL, R SCHOCH, C PURI, A CLAGUE, MJ BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI A DISSECTION OF STEPS LEADING TO VIRAL ENVELOPE PROTEIN-MEDIATED MEMBRANE-FUSION SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP BLUMENTHAL, R (reprint author), NCI,LMMB,MEMBRANE STRUCT & FUNCT SECT,BLDG 10,RM 4B56,BETHESDA,MD 20892, USA. OI Clague, Michael/0000-0003-3355-9479 NR 0 TC 44 Z9 44 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 285 EP 296 DI 10.1111/j.1749-6632.1991.tb36499.x PG 12 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00025 PM 1741588 ER PT B AU POLLARD, HB ROJAS, E PASTOR, RW ROJAS, EM GUY, HR BURNS, AL AF POLLARD, HB ROJAS, E PASTOR, RW ROJAS, EM GUY, HR BURNS, AL BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI SYNEXIN - MOLECULAR MECHANISM OF CALCIUM-DEPENDENT MEMBRANE-FUSION AND VOLTAGE-DEPENDENT CALCIUM-CHANNEL ACTIVITY - EVIDENCE IN SUPPORT OF THE HYDROPHOBIC BRIDGE HYPOTHESIS FOR EXOCYTOTIC MEMBRANE-FUSION SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP POLLARD, HB (reprint author), NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD 20892, USA. NR 0 TC 33 Z9 33 U1 1 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 328 EP 351 DI 10.1111/j.1749-6632.1991.tb36503.x PG 24 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00029 PM 1660240 ER PT B AU CHIUE, CC HUANG, SJ AF CHIUE, CC HUANG, SJ BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI MPP+ ENHANCES POTASSIUM-EVOKED STRIATAL DOPAMINE RELEASE THROUGH A OMEGA-CONOTOXIN-INSENSITIVE, TETRODOTOXIN-SENSITIVE AND NIMODIPINE-SENSITIVE CALCIUM-DEPENDENT MECHANISM SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP CHIUE, CC (reprint author), NIMH,CEREBRAL METAB LAB,BETHESDA,MD 20892, USA. NR 0 TC 18 Z9 18 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 393 EP 396 DI 10.1111/j.1749-6632.1991.tb36507.x PG 4 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00033 PM 1741592 ER PT B AU BURNS, AL MAGENDZO, K SRIVASTAVA, M ROJAS, E CULTRARO, C DELAFUENTE, M HELDMAN, J PARRA, C POLLARD, HB AF BURNS, AL MAGENDZO, K SRIVASTAVA, M ROJAS, E CULTRARO, C DELAFUENTE, M HELDMAN, J PARRA, C POLLARD, HB BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI PROPERTIES AND MODIFICATION OF RECOMBINANT HUMAN SYNEXIN (ANNEXIN VII) SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP BURNS, AL (reprint author), NIDDKD,CELL BIOL & GENET LAB,BLDG 8,ROOM 403,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 450 EP 451 DI 10.1111/j.1749-6632.1991.tb36524.x PG 2 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00050 PM 1660250 ER PT B AU KUIJPERS, GAJ LEE, G POLLARD, HB AF KUIJPERS, GAJ LEE, G POLLARD, HB BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI IMMUNOELECTRON MICROSCOPY OF THE CALCIUM-BINDING PROTEIN SYNEXIN IN ISOLATED ADRENAL CHROMAFFIN GRANULES AND CHROMAFFIN CELLS SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP KUIJPERS, GAJ (reprint author), NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 471 EP 474 DI 10.1111/j.1749-6632.1991.tb36530.x PG 4 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00056 PM 1835828 ER PT B AU LEE, G DELAFUENTE, M POLLARD, HB AF LEE, G DELAFUENTE, M POLLARD, HB BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI A BARIUM-DEPENDENT CHROMAFFIN GRANULE AGGREGATING PROTEIN FROM BOVINE ADRENAL-MEDULLA AND OTHER TISSUES SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP LEE, G (reprint author), NIDDKD,CELL BIOL & GENET,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 477 EP 479 DI 10.1111/j.1749-6632.1991.tb36532.x PG 3 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00058 PM 1741603 ER PT B AU MAGENDZO, K SHIRVAN, A POLLARD, HB BURNS, AL AF MAGENDZO, K SHIRVAN, A POLLARD, HB BURNS, AL BE STANLEY, EF NOWYCKY, MC TRIGGLE, DJ TI TISSUE-REGULATED ALTERNATIVE SPLICING OF SYNEXIN MESSENGER-RNA SO CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON CALCIUM ENTRY AND ACTION AT THE PRESYNAPTIC NERVE TERMINAL CY OCT 15-17, 1990 CL BALTIMORE, MD SP NEW YORK ACAD SCI, HOFFMANN LA ROCHE, NIMH, NATL SCI FDN, USA, MED RES & DEV COMMAND, AMER CYANAMID, BRISTOL MYERS, BURROUGHS WELLCOME, GENENTECH, HOECHST ROUSSEL PHARM RP MAGENDZO, K (reprint author), NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-686-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 635 BP 483 EP 484 DI 10.1111/j.1749-6632.1991.tb36534.x PG 2 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BW41D UT WOS:A1991BW41D00060 PM 1835829 ER PT J AU KREGER, BE SPLANSKY, GL SCHATZKIN, A AF KREGER, BE SPLANSKY, GL SCHATZKIN, A TI THE CANCER EXPERIENCE IN THE FRAMINGHAM HEART-STUDY COHORT SO CANCER LA English DT Article AB The almost 40-year records of The Framingham Heart Study (FHS) cohort were reviewed to establish the cancer experience of this noninstitutionalized group of white subjects. Diagnoses were confirmed from pathology and laboratory reports and clinical notes. Age-specific incidence rates were compared with Connecticut Surveillance, Epidemiology, and End Results (SEER) data. Among the 5209 subjects, 1201 malignancies were confirmed. Median age at diagnosis was 69 for men and 65 for women. Lung, prostate, skin, and colon accounted for more than half of men's cancers; breast, colon, and skin made up half of the women's. FHS and Connecticut SEER rates matched closely, with the same primary tumor sites appearing commonly in both groups. Thus, the FHS cohort should provide a fair database for analysis of risk factors in cancer incidence, as it has done in cardiovascular diseases. C1 BOSTON UNIV,MED CTR,EVANS MEM DEPT CLIN RES,GEN INTERNAL MED SECT,BOSTON,MA 02215. BOSTON UNIV,MED CTR,EVANS MEM DEPT CLIN RES,PREVENT MED & EPIDEMIOL SECT,BOSTON,MA 02215. FRAMINGHAM HEART DIS EPIDEMIOL STUDY,FRAMINGHAM,MA. NCI,DIV CANC PREVENT & CONTROL,CANC PREVENT STUDIES BRANCH,BETHESDA,MD 20892. FU NCI NIH HHS [5 RO1 CA39766-02]; NHLBI NIH HHS [N01-HC-38038] NR 14 TC 24 Z9 24 U1 1 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD JAN 1 PY 1991 VL 67 IS 1 BP 1 EP 6 DI 10.1002/1097-0142(19910101)67:1<1::AID-CNCR2820670102>3.0.CO;2-W PG 6 WC Oncology SC Oncology GA EQ089 UT WOS:A1991EQ08900001 PM 1845934 ER PT J AU ZIEGLER, RG JONES, CJ BRINTON, LA NORMAN, SA MALLIN, K LEVINE, RS LEHMAN, HF HAMMAN, RF TRUMBLE, AC ROSENTHAL, JF HOOVER, RN AF ZIEGLER, RG JONES, CJ BRINTON, LA NORMAN, SA MALLIN, K LEVINE, RS LEHMAN, HF HAMMAN, RF TRUMBLE, AC ROSENTHAL, JF HOOVER, RN TI DIET AND THE RISK OF INSITU CERVICAL-CANCER AMONG WHITE WOMEN IN THE UNITED-STATES SO CANCER CAUSES & CONTROL LA English DT Article DE BETA-CAROTENE; CAROTENOIDS; CERVICAL NEOPLASMS; DIET; FOLATE; NUTRIENT STATUS; VITAMIN-A; VITAMIN-C; VITAMIN SUPPLEMENTS; USA AB A case-control study of women with incident in situ and invasive cervical cancer was conducted during 1982-83 in five US areas reporting to the Comprehensive Cancer Patient Data System: Birmingham, AL; Chicago, IL; Denver, CO; Miami, FL; and Philadelphia, PA. Controls were selected by random-digit dialing and matched to invasive cases on age, race, and telephone exchange. Of the white non-Hispanic in situ cases and controls identified, 229 (78 percent) and 502 (74 percent) were successfully interviewed. Diet was assessed by asking about the usual adult frequency of consumption of 75 food items and the use of vitamin supplements. Included were the major sources of the four micronutrients postulated to reduce the risk of cervical cancer: carotenoids, vitamin A, vitamin C, and folate. Weak inverse associations between risk of in situ disease and intake of carotenoids, vitamin C, folate, fruit, and vegetables/fruits were noted but, with further analysis, these seemed attributable to residual confounding by the multiple lifestyle-related risk factors for this disease and possibly to selection bias. Vitamin A and vegetable intake were unrelated to risk. Dark yellow-orange vegetable consumption and duration of multivitamin use were each strongly related to reduced risk of in situ disease (P for trend = 0.02 and 0.002, respectively) and need to be evaluated in other studies. The absence of persuasive protective effects for the four micronutrients and the similar findings from our analysis of invasive cervical cancer do not concur with other epidemiologic studies and suggest that the role of diet and nutrition in the etiology of cervical cancer is not yet resolved. RP ZIEGLER, RG (reprint author), NCI,EXECUTIVE PLAZA N,ROOM 443,BETHESDA,MD 20892, USA. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 NR 0 TC 47 Z9 48 U1 0 U2 6 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0957-5243 J9 CANCER CAUSE CONTROL JI Cancer Causes Control PD JAN PY 1991 VL 2 IS 1 BP 17 EP 29 DI 10.1007/BF00052357 PG 13 WC Oncology; Public, Environmental & Occupational Health SC Oncology; Public, Environmental & Occupational Health GA EU909 UT WOS:A1991EU90900003 PM 1873430 ER PT J AU ROBERTS, AB KIM, SJ SPORN, MB AF ROBERTS, AB KIM, SJ SPORN, MB TI IS THERE A COMMON PATHWAY MEDIATING GROWTH-INHIBITION BY TGF-BETA AND THE RETINOBLASTOMA GENE-PRODUCT SO CANCER CELLS-A MONTHLY REVIEW LA English DT Article ID MURINE KERATINOCYTES; SUSCEPTIBILITY GENE; BETA; SV40; ACTIVATION; CELLS RP ROBERTS, AB (reprint author), NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892, USA. NR 21 TC 21 Z9 21 U1 0 U2 0 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 1042-2196 J9 CANCER CELL-MON REV PD JAN PY 1991 VL 3 IS 1 BP 19 EP 21 PG 3 WC Oncology; Medicine, Research & Experimental SC Oncology; Research & Experimental Medicine GA EX543 UT WOS:A1991EX54300004 PM 2025491 ER PT J AU TAKADA, Y GREIG, NH VISTICA, DT RAPOPORT, SI SMITH, QR AF TAKADA, Y GREIG, NH VISTICA, DT RAPOPORT, SI SMITH, QR TI AFFINITY OF ANTINEOPLASTIC AMINO-ACID DRUGS FOR THE LARGE NEUTRAL AMINO-ACID TRANSPORTER OF THE BLOOD-BRAIN-BARRIER SO CANCER CHEMOTHERAPY AND PHARMACOLOGY LA English DT Article DE BLOOD BRAIN BARRIER; AMINO ACID TRANSPORT; BRAIN TUMOR ID CENTRAL-NERVOUS-SYSTEM; PERFUSION TECHNIQUE; CEREBROVASCULAR TRANSPORT; BUTHIONINE SULFOXIMINE; CHEMOTHERAPY; ACIVICIN; TOXICITY; TUMORS; RAT; METHOTREXATE AB The relative affinity of six anticancer amino acid drugs for the neutral amino acid carrier of the blood-brain barrier was examined in rats using an in situ brain perfusion technique. Affinity was evaluated from the concentration-dependent inhibition of L-[C-14]-leucine uptake into rat brain during perfusion at tracer leucine concentrations and in the absence of competing amino acids. Of the six drugs tested, five, including melphalan, azaserine, acivicin, 6-diazo-5-oxo-L-norleucine, and buthionine sulfoximine, exhibited only low affinity for the carrier, displaying transport inhibition constants (K(i), concentrations producing 50% inhibition) ranging from 0.09 to 4.7 mM. However, one agent - D,L-2-amino-7-bis[(2-chloroethyl)amino]-1,2,3,4-tetrahydro-2-naphthoic acid (D,L-NAM) - demonstrated remarkably high affinity for the carrier, showing a K(i) value of approximately 0.2-mu-M. The relative affinity (1/K(i)) of D,L-NAM was > 100-fold that of the other drugs and > 10-fold that of any compound previously tested. As the blood-brain barrier penetrability of most endogenous neutral amino acids is related to their carrier affinity, the results suggest that D,L-NAM may be a promising agent which may show enhanced uptake and distribution to brain tumors. C1 NIA,NEUROSCI LAB,BLDG 10,ROOM 6C-103,BETHESDA,MD 20892. NCI,FREDERICK CANC RES CTR,DRUG DISCOVERY RES & DEV LAB,FREDERICK,MD 21702. NR 45 TC 42 Z9 43 U1 0 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0344-5704 J9 CANCER CHEMOTH PHARM JI Cancer Chemother. Pharmacol. PY 1991 VL 29 IS 2 BP 89 EP 94 DI 10.1007/BF00687316 PG 6 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA GU093 UT WOS:A1991GU09300001 PM 1760863 ER PT J AU MORGAN, PF FINE, RL MONTGOMERY, P MARANGOS, PJ AF MORGAN, PF FINE, RL MONTGOMERY, P MARANGOS, PJ TI MULTIDRUG RESISTANCE IN MCF-7 HUMAN BREAST-CANCER CELLS IS ASSOCIATED WITH INCREASED EXPRESSION OF NUCLEOSIDE TRANSPORTERS AND ALTERED UPTAKE OF ADENOSINE SO CANCER CHEMOTHERAPY AND PHARMACOLOGY LA English DT Article DE MULTIDRUG RESISTANCE; NUCLEOSIDE TRANSPORT; ADENOSINE; HUMAN BREAST CANCER CELLS ID PROTEIN KINASE-C; LYSOSOMAL-ENZYME RELEASE; P-GLYCOPROTEIN; MONOCLONAL-ANTIBODIES; NORADRENALINE RELEASE; ADENYLATE-CYCLASE; RAT HIPPOCAMPUS; PHORBOL ESTERS; TUMOR-CELLS; PHOSPHORYLATION AB The rate of adenosine uptake and the corresponding expression of nucleoside transporters were studied in several MCF-7 human breast-cancer cell lines that express different levels of multidrug resistance (MDR). Kinetic studies of adenosine transport in these cell lines revealed that the mean apparent K(m) and V(max) values for the nucleoside transporters increased with increasing MDR. The apparent K(m) and the apparent V(max) of Adriamycin-resistant (ADR10) cell lines were respectively 3.2- and 1.8-fold those of Adriamycin-sensitive wild-type (WT) cells (P < 0.001). A partially revertant cell line (ADR10rev) that was derived from the ADR10 line and was partially sensitive to Adriamycin exhibited apparent K(m) and V(max) parameters that lay between those of the ADR10 and WT cells (P < 0.001 vs ADR10 cells; P < 0.05 vs WT cells). ADR10 cell membranes bound > 4 times more of the nucleoside transporter blockers [H-3]-nitrobenzylthioinosine ([H-3]-NBI) and [H-3]-dipyridamole ([H-3]-DPR) than did WT cell membranes per unit protein (P < 0.0001). Scatchard analysis revealed a 2-3 times greater density for nucleoside transporters in ADR10 membranes as compared with those in WT membranes. ADR10rev membranes bound less [H-3]-NBI and [H-3]-DPR than did ADR10 membranes (P < 0.001), but they bound more of the blockers than did WT membranes (P < 0.05). A 2.5-h exposure to 200 nM phorbol-12,13-dibutyrate (PDBu), which activates protein kinase C (PKC) and induces WT cells to exhibit a 4-fold increased transient MDR phenotype, increased the apparent K(m) of WT cells for adenosine transport by > 2 times (P < 0.001) to a value close to that found for the ADR10 cells. An identical exposure of ADR10 cells to PDBu produced no significant effect. The apparent K(M) of ADR10rev cells was increased 1.4 times by a 2.5-h PDBu exposure. None of the cell lines were affected by a 2.5-h exposure to 200 nM phorbol-13,10-diacetate (PDA), a much less active phorbol, or vehicle. These results suggest that MDR in MCF-7 cells is associated with changes in nucleoside transport, including both the number of transporters and their rate of transport, and that such changes can be partially mimicked by stimulation of PKC. C1 NCI,DIV CANC TREATMENT,MED BRANCH,BETHESDA,MD 20892. NIMH,BIOL PSYCHIAT BRANCH,NEUROCHEM UNIT,BETHESDA,MD 20892. RP MORGAN, PF (reprint author), NIAAA,CLIN STUDIES LAB,BLDG 10,ROOM 3C-102,BETHESDA,MD 20892, USA. NR 31 TC 17 Z9 18 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0344-5704 J9 CANCER CHEMOTH PHARM JI Cancer Chemother. Pharmacol. PY 1991 VL 29 IS 2 BP 127 EP 132 DI 10.1007/BF00687322 PG 6 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA GU093 UT WOS:A1991GU09300007 PM 1760855 ER PT J AU CONLEY, BA SEWACK, GF EGORIN, MJ SUBRAMANYAM, B PAGE, JG GRIESHABER, CK AF CONLEY, BA SEWACK, GF EGORIN, MJ SUBRAMANYAM, B PAGE, JG GRIESHABER, CK TI THE EFFECT OF THE MONOAMINE-OXIDASE INHIBITOR ISOCARBOXAZID ON THE CANINE METABOLISM OF THE CELL-DIFFERENTIATING AGENT HEXAMETHYLENE BISACETAMIDE SO CANCER CHEMOTHERAPY AND PHARMACOLOGY LA English DT Article ID MURINE ERYTHROLEUKEMIA CELLS; PHASE-I TRIAL; LEUKEMIA-CELLS; INDUCTION; INFUSION; NSC-95580; INDUCERS; HL60 AB The acute toxicities of the cellular differentiating agent hexamethylene bisacetamide (HMBA) in humans and animals include CNS toxicity (agitation, somnolence, seizures, hallucinations) and an anion-gap metabolic acidosis. N-Acetyl-1,6-diaminohexane (NADAH), the first metabolite of HMBA, is as active as the parent compound in causing differentiation of leukemic cells in vitro, whereas 6-acetamidohexanoic acid (6AcHA), which is formed by the oxidation of NADAH in the presence of monoamine oxidase (MAO) and aldehyde dehydrogenase, is inactive. To test whether the inhibition of MAO blocks the production of an inactive and possibly toxic HMBA metabolite (6AcHA) or increases the amount of active compounds (HMBA + NADAH) in vivo, we investigated the effect of the MAO inhibitor isocarboxazid on the metabolism and toxicity of HMBA in beagle dogs. Two groups of dogs, composed of one male and one female dog per group, were used in the study. One group received isocarboxazid (3.3 mg/kg p.o. q8h x9) beginning at 24 h before the initiation of a 48-h i.v. infusion of HMBA (40 mg kg-1 h-1), whereas the other received placebo in an identifical fashion prior to the start of an identical HMBA infusion. The mean plasma steady-state concentration (C(ss)) of HMBA was 0.91 mM in dogs given HMBA and isocarboxazid as opposed to 0.78 mM in those given HMBA and placebo. As measured spectrophotometrically, plasma MAO activity was inhibited by 86% +/- 3% in dogs receiving isocarboxazid. Gas chromatography/mass spectrometry detected 6AcHA in the plasma of animals that were given placebo but not in the plasma of dogs that received isocarboxazid. Gas chromatographic analysis of urine samples revealed that the total amount of 6AcHA and of NADAH excreted in urine was 8 times less and 3 times greater, respectively, in isocarboxazid-treated dogs than in animals that received HMBA and placebo. One dog was excitable after the initial two doses of isocarboxazid and developed seizures at the end of the HMBA infusion. Another dog was agitated during treatment with HMBA and isocarboxazid. No CNS toxicity occurred in animals that were treated with HMBA and placebo. We conclude that isocarboxazid inhibits the production of 6AcHA in vivo, thus supporting the involvement of MAO in HMBA metabolism. Because the combination of HMBA and isocarboxazid produces CNS toxicity, 6AcHA is probably not the neurotoxic agent in dogs. C1 UNIV MARYLAND,CTR CANC,DIV MED ONCOL,BALTIMORE,MD 21201. UNIV MARYLAND,SCH MED,DEPT MED,DIV MED ONCOL,BALTIMORE,MD 21201. SCH PHARM,BALTIMORE,MD. NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,TOXICOL BRANCH,BETHESDA,MD 20892. BATTELLE COLUMBUS DIV,COLUMBUS,OH. RP CONLEY, BA (reprint author), UNIV MARYLAND,CTR CANC,DIV DEV THERAPEUT,BRESSLER RES BLDG,ROOM 9-0196,655 W BALTIMORE ST,BALTIMORE,MD 21201, USA. FU NCI NIH HHS [N-01-CN-57734, N01-CM-17365] NR 25 TC 3 Z9 3 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0344-5704 J9 CANCER CHEMOTH PHARM JI Cancer Chemother. Pharmacol. PY 1991 VL 28 IS 1 BP 33 EP 38 DI 10.1007/BF00684953 PG 6 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA FL749 UT WOS:A1991FL74900006 PM 2040031 ER PT J AU KAMATH, N GRABOWSKI, D FORD, J DRAKE, F KERRIGAN, D POMMIER, Y GANAPATHI, R AF KAMATH, N GRABOWSKI, D FORD, J DRAKE, F KERRIGAN, D POMMIER, Y GANAPATHI, R TI TRIFLUOPERAZINE MODULATION OF RESISTANCE TO THE TOPOISOMERASE-II INHIBITOR ETOPOSIDE IN DOXORUBICIN RESISTANT L1210 MURINE LEUKEMIA-CELLS SO CANCER COMMUNICATIONS LA English DT Article ID DNA STRAND BREAKS; MULTIDRUG RESISTANCE; CYTO-TOXICITY; CLEAVAGE; PROTEINS; LINE AB The present study was performed to characterize the results of exposure of the sensitive (S) and progressively DOX-resistant (10-fold, R1, and 40-fold, R2) L1210 cells to the topoisomerase II inhibitor, etoposide, and to investigate the modulating effects of the calmodulin inhibitor, trifluoperazine (TFP). Immunoblotting experiments indicated no apparent decrease in the p170 or p180 isoforms of topoisomerase II in the resistant sublines versus parental sensitive cells. Cross-resistance to etoposide (VP-16) was similar to that of DOX (10- and 40-fold). A non-cytotoxic concentration of 5-mu-M TFP enhanced cell kill 1.5-fold in the sensitive and 3- to 5-fold in the progressively DOX-resistant cells. Accumulation of VP-16 was 30% to 50% lower in the resistant sublines versus similarly treated sensitive cells, and a marked enhancement of drug uptake in the presence of TFP was observed in the sensitive but not in the resistant cells exposed to equivalent extracellular levels of VP-16. Although equimolar concentrations of VP-16 produced fewer DNA single strand breaks (SSB) and DNA protein crosslinks (DPC) in the resistant versus sensitive cells, similar DNA damage was apparent when S and R1, but not R2, cells were treated at VP-16 concentrations that produced equivalent cell death. In agreement with the cytotoxicity data, induction of SSB and DPC by VP-16 was also enhanced by a similar magnitude in the presence of TFP. Equimolar concentrations of VP-16 produced significantly fewer SSB in isolated nuclei from the resistant versus sensitive cells. Unlike whole cells, however, no modulation by TFP of VP-16 induced DNA damage was apparent in isolated nuclei, suggesting that TFP did not act directly on topoisomerase II. The results demonstrated that VP-16 resistance in progressively DOX-resistant L1210 cell lines, which exhibited alterations in activity but not in levels of topoisomerase II, directly correlated with reductions in drug induced DNA damage. The efficacy of the calmodulin inhibitor trifluoperazine in modulating VP-16 induced DNA damage and consequently cytotoxicity, without effects in isolated nuclei or increasing cellular drug accumulation, suggests that mechanisms related to extranuclear events affecting topoisomerase II activity may have been involved. C1 CLEVELAND CLIN FDN,RES INST,9500 EUCLID AVE,CLEVELAND,OH 44195. NCI,DIV CANC TREATMENT,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. SMITH KLINE BEECHAM PHARMACEUT,DEPT CELL SCI,KING OF PRUSSIA,PA 19406. FU NCI NIH HHS [R01CA35531] NR 25 TC 8 Z9 8 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0955-3541 J9 CANCER COMMUN PY 1991 VL 3 IS 2 BP 37 EP 44 PG 8 WC Oncology; Medicine, Research & Experimental SC Oncology; Research & Experimental Medicine GA EY839 UT WOS:A1991EY83900002 PM 1995027 ER PT J AU LEWIN, NE PETTIT, GR KAMANO, Y BLUMBERG, PM AF LEWIN, NE PETTIT, GR KAMANO, Y BLUMBERG, PM TI BINDING OF [26-H-3]-EPI-BRYOSTATIN-4 TO PROTEIN-KINASE-C SO CANCER COMMUNICATIONS LA English DT Article ID PHORBOL ESTER PHARMACOPHORE; TUMOR PROMOTERS; RECEPTOR; TELEOCIDIN; APLYSIATOXINS; BRYOSTATIN-1; ACTIVATION AB Structure activity analysis of protein kinase C modulators may permit design of selective inhibitors of this important regulatory enzyme. Modeling suggests that the C-26 secondary hydroxyl of bryostatin is homologous to the C-20 primary hydroxyl of phorbol or the C-3 primary hydroxyl of sn-1,2-diacylglycerols (Wender, P. A.; Cribbs, C. M.; Koehler, K. F.; Sharkey, N. A.; Herald, C. L.; Kamano, Y.; Pettit, G. R.; Blumberg, P. M. Modeling of the bryostatins to the phorbol ester pharmacophore on protein kinase C. Proc. Natl. Acad. Sci. USA 85:7197-7201; 1980). We have characterized the binding activity to protein kinase C of the epimer of bryostatin 4 with the configuration at C-26 inverted from R (natural configuration) to S. The K(d) of [26-H-3]-epi-bryostatin 4 for protein kinase C reconstituted in the presence of phosphatidylserine was 13 +/- 2 nM. [26-H-3]-Epi-bryostatin 4 thus retained moderate absolute affinity. Relatively, however, inversion at the chiral center at C-26 caused a dramatic decrease in binding affinity. Binding of [26-H-3]-epi-bryostatin 4 was competitively inhibited by phorbol 12, 13-diacetate, as expected for ligands that interact at the same binding site. C1 NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892. ARIZONA STATE UNIV,CANC RES INST,TEMPE,AZ 85287. FU NCI NIH HHS [CA-44344-01A1, CA-16049-10-12]; NIAID NIH HHS [AI-25696-02] NR 23 TC 14 Z9 14 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0955-3541 J9 CANCER COMMUN PY 1991 VL 3 IS 3 BP 67 EP 70 PG 4 WC Oncology; Medicine, Research & Experimental SC Oncology; Research & Experimental Medicine GA FB901 UT WOS:A1991FB90100002 PM 2001329 ER PT J AU YU, G AHMAD, S AQUINO, A FAIRCHILD, CR TREPEL, JB OHNO, S SUZUKI, K TSURUO, T COWAN, KH GLAZER, RI AF YU, G AHMAD, S AQUINO, A FAIRCHILD, CR TREPEL, JB OHNO, S SUZUKI, K TSURUO, T COWAN, KH GLAZER, RI TI TRANSFECTION WITH PROTEIN-KINASE C-ALPHA CONFERS INCREASED MULTIDRUG RESISTANCE TO MCF-7 CELLS EXPRESSING P-GLYCOPROTEIN SO CANCER COMMUNICATIONS LA English DT Article ID HL-60 LEUKEMIA-CELLS; BREAST CANCER-CELLS; PLASMA-MEMBRANE GLYCOPROTEIN; KB CARCINOMA-CELLS; BACTERIAL TRANSPORT PROTEINS; MULTIPLE-DRUG RESISTANCE; DNA-MEDIATED TRANSFER; HUMAN MDR1 GENE; TUMOR-CELLS; ADRIAMYCIN RESISTANCE AB Cross-resistance to anticancer drugs, termed multidrug resistance (mdr), has been functionally associated with the expression of a plasma membrane energy-dependent efflux pump, termed P-glycoprotein, the product of the mdr1 gene. When MCF-7 breast carcinoma cells were transfected with the human mdr1 gene (BC-19 cells), they expressed levels of P-glycoprotein equivalent to those of cells selected for resistance to doxorubicin (MCF-7/ADR) but exhibited 10- to 50-fold less resistance to doxorubicin and vinblastine. We have now demonstrated that when BC-19 cells were stably transfected with protein kinase C-alpha (PKC-alpha), resistance to doxorubicin and vinblastine was increased; wild-type MCF-7 cells transfected with PKC-alpha did not exhibit any change in drug resistance. Increased resistance in PKC-alpha-transfected BC-19 cells was associated with enhanced PKC activity and phosphorylation of P-glycoprotein and decreased drug accumulation. The PKC activator, phorbol dibutyrate, further increased resistance to doxorubicin and stimulated P-glycoprotein phosphorylation. These results demonstrate that transfection of P-glycoprotein-expressing cells with PKC resulted in increased mdr and that PKC may have served as an important modulator of this process. C1 NCI,BIOL CHEM LAB,BETHESDA,MD 20892. RI Ohno, Shigeo/B-1768-2010 OI Ohno, Shigeo/0000-0002-1294-5269 NR 55 TC 160 Z9 166 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0955-3541 J9 CANCER COMMUN PY 1991 VL 3 IS 6 BP 181 EP 189 PG 9 WC Oncology; Medicine, Research & Experimental SC Oncology; Research & Experimental Medicine GA FV440 UT WOS:A1991FV44000003 PM 1675575 ER PT J AU OCONNOR, PM NIEVESNEIRA, W KERRIGAN, D BERTRAND, R GOLDMAN, J KOHN, KW POMMIER, Y AF OCONNOR, PM NIEVESNEIRA, W KERRIGAN, D BERTRAND, R GOLDMAN, J KOHN, KW POMMIER, Y TI S-PHASE POPULATION ANALYSIS DOES NOT CORRELATE WITH THE CYTOTOXICITY OF CAMPTOTHECIN AND 10,11-METHYLENEDIOXYCAMPTOTHECIN IN HUMAN COLON-CARCINOMA HT-29 CELLS SO CANCER COMMUNICATIONS LA English DT Article ID DNA TOPOISOMERASE-I; STRAND BREAKS; L1210 CELLS; ANTITUMOR DRUGS; REPLICATION; PHARMACOKINETICS; INHIBITOR; ABSENCE; CYCLE AB Previous studies in rapidly proliferating rodent cells have suggested that the lethal effect of the DNA topoisomerase I inhibitor, camptothecin (CPT) is dependent upon the active participation of DNA replication (Holm et al. Cancer Res. 49:6365-6368; 1989). The purpose of the current study was to determine if this relationship applies to more slowly growing human cells. In our present study, we employed the human colon carcinoma cell line, HT-29 (45 hr doubling time). Flow cytometric determination of S-phase cells either by S-phase fit model or rectangle fit model analysis predicted that 21% of exponentially growing HT-29 cells were undergoing DNA replication. These findings were confirmed by immunofluorescence microscopy of bromodeoxyuridine labeled cells. Based on these findings, we would have expected only 20-30% of the cells to be susceptible to brief treatment (30 min) with CPT. Instead, 90-95% of HT-29 cells were killed. This apparent disparity was not due to prolonged cellular retention of drug after treatment because protein-linked DNA strand breaks reversed within 15 min of drug removal. Moreover, the DNA replication inhibitor, aphidicolin, fully protected HT-29 cells against CPT-induced killing but did not affect the production of CPT-induced protein-linked DNA strand breaks. Similar results were also obtained using the CPT-analog, 10,11-methylenedioxycamptothecin, which was 5- to 10-fold more potent than camptothecin (O'Connor et al. Cancer Commun. 2:395-400; 1990). Our findings imply that replication events actively participate in HT-29 cell killing by the campotothecins and that CPT also exhibits actions outside of the processes of DNA elongation, presumably extending through most of G1 in HT-29 cells, where molecular events leading to DNA replication are initiated. C1 NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. NR 28 TC 41 Z9 42 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0955-3541 J9 CANCER COMMUN PY 1991 VL 3 IS 8 BP 233 EP 240 PG 8 WC Oncology; Medicine, Research & Experimental SC Oncology; Research & Experimental Medicine GA GG547 UT WOS:A1991GG54700001 PM 1653585 ER PT J AU TOWNSEND, AJ MORROW, CS SINHA, BK COWAN, KH AF TOWNSEND, AJ MORROW, CS SINHA, BK COWAN, KH TI SELENIUM-DEPENDENT GLUTATHIONE-PEROXIDASE EXPRESSION IS INVERSELY RELATED TO ESTROGEN-RECEPTOR CONTENT OF HUMAN BREAST-CANCER CELLS SO CANCER COMMUNICATIONS LA English DT Article ID FREE-RADICALS; MULTIDRUG RESISTANCE; GENE-EXPRESSION; TUMOR-CELLS; TRANSFERASE; ADRIAMYCIN; CYTOTOXICITY; INVIVO; CYCLE; HEART AB The absence of estrogen receptors (ER) in human breast tumors has been associated with a poorer prognosis compared to patients with ER positive breast cancer. Previous studies from our laboratory have shown that a multidrug resistant human breast cancer cell line selected for resistance to Adriamycin (ADR) exhibited markedly increased expression of both the pi-class glutathione S-transferase (GST-pi) and the selenium-dependent glutathione peroxidase. These studies also revealed that the ER status was inversely related to the expression of GST-pi in six human breast cancer cell lines and primary tumor specimens. In the present study, we have examined the relationship between ER status and several biological properties of these cells, including their levels of glutathione peroxidase (GSH-Px) and catalase expression, their capacity to generate toxic hydroxyl radicals (degrees-OH) by redox cycling of ADR, and their sensitivities to the cytotoxic effects of ADR and the oxidant, H2O2. Our results show that expression of GSH-Px, but not catalase, is inversely related to the ER status in these cell lines. Formation of the degrees-OH induced by treatment of cells with ADR was inversely proportional to the GSH-Px activity in these cell lines, and thus directly related to the ER status. Sensitivity of these cells to ADR or to H2O2, however, was not consistently related to ER status, GSH-Px, or catalase activity, or to ADR induced degrees-OH radical formation. These results indicate that these parameters are not predictive of cellular susceptibility to oxidative damage in these cell lines under the conditions studied. C1 NCI,MED BRANCH,BETHESDA,MD 20892. NR 33 TC 13 Z9 13 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0955-3541 J9 CANCER COMMUN PY 1991 VL 3 IS 8 BP 265 EP 270 PG 6 WC Oncology; Medicine, Research & Experimental SC Oncology; Research & Experimental Medicine GA GG547 UT WOS:A1991GG54700004 PM 1653587 ER PT J AU HENNINGS, H ROBINSON, VA YUSPA, SH WELTY, DJ GREENHALGH, DG AF HENNINGS, H ROBINSON, VA YUSPA, SH WELTY, DJ GREENHALGH, DG TI ACTIVATION OF THE RASHA GENE IN URETHANE-INITIATED PAPILLOMAS INDUCED BY PROTOCOLS WITH HIGH AND LOW-FREQUENCIES OF MALIGNANT CONVERSION SO CANCER COMMUNICATIONS LA English DT Article ID MOUSE SKIN TUMORS; PRIMARY MURINE KERATINOCYTES; HA-RAS; MUTATIONS; PROTOONCOGENE; PROGRESSION; CARCINOGENS; ONCOGENE; DNA AB The number of papillomas that develop in mice initiated with a single exposure to urethane and promoted by repeated applications of 12-O-tetradecanoylphorbol-13-acetate (TPA) is increased > 4-fold by pretreating the skin once with TPA 24 hr before administration of urethane. In contrast, the carcinoma incidence was increased only 2-fold by the TPA pretreatment. Individual papillomas developed from the two protocols, differing in the potential for conversion to malignancy, were compared for activation of the ras(Ha) gene. An activated oncogene that transformed 3T3 cells was found in the DNA of four of five papillomas from urethane-initiated, TPA-promoted mice and in eight of eleven papillomas from similarly promoted mice exposed to TPA before urethane initiation. Southern analysis of DNA from tumors or 3T3 foci demonstrated that the ras(Ha) gene was activated by an A --> T transversion at the second base of codon 61 in all mutated alleles. Thus, tumors induced by the two protocols did not differ in ras(Ha) activation. RP HENNINGS, H (reprint author), NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT,BLDG 37,ROOM 3B26,BETHESDA,MD 20892, USA. NR 22 TC 6 Z9 6 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0955-3541 J9 CANCER COMMUN PY 1991 VL 3 IS 9 BP 277 EP 281 PG 5 WC Oncology; Medicine, Research & Experimental SC Oncology; Research & Experimental Medicine GA GM009 UT WOS:A1991GM00900002 PM 1911043 ER PT J AU DIXON, KH TREPEL, JB ENG, SC COWAN, KH AF DIXON, KH TREPEL, JB ENG, SC COWAN, KH TI FOLATE TRANSPORT AND THE MODULATION OF ANTIFOLATE SENSITIVITY IN A METHOTREXATE-RESISTANT HUMAN BREAST-CANCER CELL-LINE SO CANCER COMMUNICATIONS LA English DT Article ID HAMSTER OVARY CELLS; CCRF-CEM CELLS; HUMAN KB CELLS; BINDING-PROTEIN; L1210 CELLS; MEMBRANE-TRANSPORT; LEUKEMIA-CELLS; FLOW-CYTOMETRY; TUMOR-CELLS; ANTI-FOLATE AB The mechanism of acquired methotrexate-resistance in an estrogen-receptor positive human breast cancer cell line (MTX(R)ZR-75-1) was studied. MTX(R)ZR-75-1 cells are 250-fold resistant to methotrexate when grown in the presence of 1-mu-M folinic acid and 2,400-fold resistant in the presence of 1-mu-M folic acid. This drug resistant cell line also showed collateral sensitivity (10-fold) to trimetrexate (TMQ), when grown in the presence of folinic acid. Using fluoresceinated methotrexate (F-MTX), FACS analysis indicated that there is no intracellular accumulation of methotrexate into MTX(R)ZR-75-1 cells, as determined by competition of F-MTX and methotrexate binding to dihydrofolate reductase. These characteristics strongly indicate that the mechanism of resistance involved down regulation of the reduced-folate transporter. To investigate this further, the transport kinetics of parental and MTX(R)ZR-75-1 cells were examined. Although the V(max) for methotrexate transport in wild-type (WT) ZR-75-1 breast cancer cells was 1-2 orders of magnitude lower than that in the well characterized leukemia cell lines, such as L1210 and CCRF-CEM cells, kinetic analysis indicated that transport of methotrexate into WT ZR-75-1 cells involved a mechanism that was similar if not identical to the reduced folate transporter. In contrast, no specific uptake of methotrexate was detected in MTX(R)ZR-75-1 cells. Furthermore, neither cell line expressed detectable levels of folate binding protein, a binding protein with high affinity for folic acid as well as for reduced folates and antifolates. These results indicate that the level of expression of the reduced-folate carrier may be an important factor in determining the sensitivity of breast cancer cells as well as leukemia cells to antifolate compounds. C1 NCI,MED BRANCH,BLDG 10,ROOM 12N226,BETHESDA,MD 20892. NCI,CLIN PHARMACOL BRANCH,BETHESDA,MD 20892. NR 44 TC 21 Z9 21 U1 0 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0955-3541 J9 CANCER COMMUN PY 1991 VL 3 IS 12 BP 357 EP 365 PG 9 WC Oncology; Medicine, Research & Experimental SC Oncology; Research & Experimental Medicine GA HJ669 UT WOS:A1991HJ66900001 PM 16296001 ER PT J AU NIERODA, CA MOJZISIK, C HINKLE, G THURSTON, MO MARTIN, EW AF NIERODA, CA MOJZISIK, C HINKLE, G THURSTON, MO MARTIN, EW TI RADIOIMMUNOGUIDED SURGERY (RIGS) IN RECURRENT COLORECTAL-CANCER SO CANCER DETECTION AND PREVENTION LA English DT Article DE RADIOIMMUNOGUIDED SURGERY; RECURRENT COLORECTAL CARCINOMA; 2ND-LOOK SURGERY; MONOCLONAL ANTIBODY B72.3; MONOCLONAL ANTIBODY 17-1A; GAMMA DETECTING PROBE AB Since 1986, 191 patients with recurrent colorectal cancer have undergone surgical exploration 2 to 43 days after injection of 1.0 to 0.25 mg of monoclonal antibody (MAb) (B72.3 or 17-1A) radiolabeled with 5.0 to 1.0 mCi of I-125. The intraoperative use of a hand-held gamma detector (Neoprobe(R) 1000) demonstrated that MAb identified tumor in 73% of cases. Clearer intraoperative definition of tumor margins and identification of occult tumor assisted the surgeon in the resection of liver metastases as well as nodal and pelvic disease. Unsuspected nodal disease was identified. The external use of the Neoprobe to scan the sacral region and intrarectal and intravaginal use led to the avoidance of operative procedures by defining inoperable disease. In approximately 25% of cases, the surgical procedure was modified based on Neoprobe findings. RIGS(R) system provides a method of immediate intraoperative staging which may prevent additional recurrences, lead to earlier institution of adjuvant therapy, and result in improved survival. RP NIERODA, CA (reprint author), NCI,BLDG 10,ROOM 8B08,BETHESDA,MD 20892, USA. RI Martin Jr, Edward/E-3604-2011 NR 0 TC 17 Z9 17 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0361-090X J9 CANCER DETECT PREV JI Cancer Detect. Prev. PY 1991 VL 15 IS 3 BP 225 EP 229 PG 5 WC Oncology SC Oncology GA FM926 UT WOS:A1991FM92600010 PM 2059962 ER PT J AU COHEN, MH ELIN, RJ COHEN, BJ AF COHEN, MH ELIN, RJ COHEN, BJ TI HYPOTENSION AND DISSEMINATED INTRAVASCULAR COAGULATION FOLLOWING INTRALESIONAL BACILLUS CALMETTE-GUERIN THERAPY FOR LOCALLY METASTATIC MELANOMA SO CANCER IMMUNOLOGY IMMUNOTHERAPY LA English DT Article ID CUTANEOUS MALIGNANT-MELANOMA; BCG IMMUNOTHERAPY; LETHAL ENDOTOXEMIA; LIMULUS TEST; COMPLICATIONS; INJECTION; HYPERSENSITIVITY; SHOCK AB Four patients developed serious hypotension and signs of disseminated intravascular coagulation shortly after a second round of Tice bacillus Calmette-Guerin (BCG) injections into locally recurrent cutaneous melanoma satellite nodules. Each of these patients survived following intensive therapy with isoniazid, pyridoxine, steroids, pressors, antibiotics, and cardio-renal support including, in one case, three acute hemodialyses. Plasma specimens from two of the four patients caused gelation of lysate from the amebocytes of Limulus polyphemus, indicating the presence of endotoxin or an endotoxin-like substance. In vitro studies on the BCG preparations led us to conclude that this endotoxin activity in the plasma is not the result of direct injection of endotoxin with the BCG preparation, but rather from release of endotoxin from endogenous sources, such as the intestinal tract during a period of relative hypotension following an allergic reaction. Prior immunity appeared to be the consistent factor in the toxic reactions reported herein. Finally, we present recommendations for serial monitoring of these patients and discuss the use of an alternative agent for intralesional therapy. C1 GEORGE WASHINGTON UNIV,SCH MED,WASHINGTON,DC 20052. NIH,WARREN GRANT MAGNESON CLIN CTR,DEPT CLIN PATHOL,BETHESDA,MD 20892. WASHINGTON HOSP CTR,WASHINGTON,DC 20010. NASSAU CTY MED CTR,E MEADOW,NY 11554. NATL REHABIL HOSP,WASHINGTON,DC. RP COHEN, MH (reprint author), WASHINGTON HOSP CTR,106 IRVING ST NW,SUITE 301,WASHINGTON,DC 20010, USA. NR 41 TC 8 Z9 9 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-7004 J9 CANCER IMMUNOL IMMUN JI Cancer Immunol. Immunother. PD JAN PY 1991 VL 32 IS 5 BP 315 EP 324 DI 10.1007/BF01789050 PG 10 WC Oncology; Immunology SC Oncology; Immunology GA EU566 UT WOS:A1991EU56600008 PM 1998973 ER PT J AU TABOR, E AF TABOR, E TI CIRCULATING IMMUNE-COMPLEXES IN HEPATOCELLULAR-CARCINOMA SO CANCER INVESTIGATION LA English DT Editorial Material ID B SURFACE-ANTIGEN; CHRONIC LIVER-DISEASE; COMPLEMENT ACTIVATION; VIRUS-INFECTION; HEPATITIS; GLOMERULONEPHRITIS; DNA; CIRRHOSIS; CHILDREN RP TABOR, E (reprint author), NCI,BETHESDA,MD 20892, USA. NR 17 TC 1 Z9 1 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0735-7907 J9 CANCER INVEST JI Cancer Invest. PY 1991 VL 9 IS 2 BP 241 EP 242 DI 10.3109/07357909109044235 PG 2 WC Oncology SC Oncology GA GA046 UT WOS:A1991GA04600015 PM 1650627 ER PT J AU LIOTTA, LA STETLERSTEVENSON, WG STEEG, PS AF LIOTTA, LA STETLERSTEVENSON, WG STEEG, PS TI CANCER INVASION AND METASTASIS - POSITIVE AND NEGATIVE REGULATORY ELEMENTS SO CANCER INVESTIGATION LA English DT Article ID FIBROBLAST COLLAGENASE INHIBITOR; BRONCHIAL EPITHELIAL-CELLS; BASEMENT-MEMBRANE COLLAGEN; HUMAN AMNIOTIC MEMBRANE; TUMOR-METASTASIS; IV COLLAGENASE; METALLOPROTEINASE; PURIFICATION; ONCOGENE; FAMILY RP LIOTTA, LA (reprint author), NCI,PATHOL LAB,BETHESDA,MD 20892, USA. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 38 TC 128 Z9 130 U1 0 U2 6 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0735-7907 J9 CANCER INVEST JI Cancer Invest. PY 1991 VL 9 IS 5 BP 543 EP 551 DI 10.3109/07357909109018952 PG 9 WC Oncology SC Oncology GA GN592 UT WOS:A1991GN59200007 PM 1933487 ER PT J AU DEVOR, DE HENNEMAN, JR KEEFER, LK LOGSDON, DL RICE, JM STREETER, AJ WARD, JM AF DEVOR, DE HENNEMAN, JR KEEFER, LK LOGSDON, DL RICE, JM STREETER, AJ WARD, JM TI CARCINOGENICITY STUDY OF FECAPENTAENE-12 DIACETATE ON SKIN PAINTING IN SENCAR MICE SO CANCER LETTERS LA English DT Article DE FECAPENTAENE-12 DIACETATE; TUMOR INITIATION STUDY; ALPHA-TOCOPHEROL ID HUMAN FECES; PRECURSORS AB To investigate the effects of both diol esterification and coadministration with antioxidant on the tumorigenicity of fecapentaene-12 (FP-12) preparations, diacetylfecapentaene-12 (DAFP-12) in dimethylsulfoxide (DMSO) was applied to SENCAR mouse skin with or without the stabilizer, vitamin E, twice/week for 5 weeks, following which all animals were promoted for up to 25 weeks by weekly applications of 12-O-tetradecanoylphorbol-13-acetate (TPA). While positive controls receiving 7,12-dimethylbenz-[a]anthracene (DMBA) instead of DAFP-12 in a similar protocol all developed papillomas (average of 23/animal), papilloma incidence in mice given DAFP-12 did not differ significantly from that of the vehicle control. We conclude that DAFP-12 shows little or no tumor initiating activity for mouse skin even when coadministered with vitamin E. C1 NCI,FREDERICK CANC RES & DEV CTR,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,ANIM SCI PROGRAM,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,BIOL CARCINOGENESIS LAB,FREDERICK,MD 21702. RI Keefer, Larry/N-3247-2014 OI Keefer, Larry/0000-0001-7489-9555 FU NCI NIH HHS [N01-CO-74102] NR 19 TC 4 Z9 4 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3835 J9 CANCER LETT JI Cancer Lett. PD JAN PY 1991 VL 56 IS 1 BP 11 EP 15 DI 10.1016/0304-3835(91)90187-M PG 5 WC Oncology SC Oncology GA EZ783 UT WOS:A1991EZ78300002 PM 2004349 ER PT J AU PRIOR, TI HELMAN, LJ FITZGERALD, DJ PASTAN, I AF PRIOR, TI HELMAN, LJ FITZGERALD, DJ PASTAN, I TI CYTOTOXIC ACTIVITY OF A RECOMBINANT FUSION PROTEIN BETWEEN INSULIN-LIKE GROWTH FACTOR-I AND PSEUDOMONAS EXOTOXIN SO CANCER RESEARCH LA English DT Article ID HUMAN-BREAST CANCER; CELL LUNG-CANCER; RECEPTOR INHIBITS GROWTH; CYTO-TOXIC ACTIVITY; FACTOR IGF BINDING; SOMATOMEDIN-C; MESSENGER-RNA; WILMS TUMOR; GENOMIC SEQUENCES; COLON CARCINOMAS AB A chimeric toxin in which the cell binding domain of Pseudomonas exotoxin was replaced with mature human insulin-like growth factor I (IGF-I) was produced in Escherichia coli. This protein, IGF-I-PE40, was cytotoxic to human cell lines derived from a variety of tumor types, with a breast carcinoma line (MCF-7) and two hepatoma lines (HEP3B and HEPG2) showing the highest sensitivity to the toxin. The specificity of IGF-I-PE40 cytotoxicity was confirmed through competition with excess IGF-I and through blockage of toxin binding using an antibody specific to the type I IGF receptor. A potential interaction between the toxin and soluble IGF-binding proteins was also demonstrated. IGF-I-PE40 may be useful in the selective elimination of cells bearing the type I IGF receptor. C1 NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. NCI,DIV CANC TREATMENT,PEDIAT BRANCH,BETHESDA,MD 20892. NR 68 TC 32 Z9 32 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 1 PY 1991 VL 51 IS 1 BP 174 EP 180 PG 7 WC Oncology SC Oncology GA EQ683 UT WOS:A1991EQ68300030 PM 1846308 ER PT J AU HUTZELL, P KASHMIRI, S COLCHER, D PRIMUS, FJ HAND, PH ROSELLI, M FINCH, M YARRANTON, G BODMER, M WHITTLE, N KING, D LOULLIS, CC MCCOY, DW CALLAHAN, R SCHLOM, J AF HUTZELL, P KASHMIRI, S COLCHER, D PRIMUS, FJ HAND, PH ROSELLI, M FINCH, M YARRANTON, G BODMER, M WHITTLE, N KING, D LOULLIS, CC MCCOY, DW CALLAHAN, R SCHLOM, J TI GENERATION AND CHARACTERIZATION OF A RECOMBINANT/CHIMERIC B72.3 (HUMAN GAMMA-1) SO CANCER RESEARCH LA English DT Article ID MONOCLONAL-ANTIBODY B72.3; TUMOR-ASSOCIATED ANTIGENS; CARCINOEMBRYONIC ANTIGEN; CHIMERIC ANTIBODIES; IGG SUBCLASSES; CARCINOMA; CELLS; SEQUENCES; TISSUES; DNA AB We report here the generation and characterization of a recombinant/chimeric construct of murine gamma-1 monoclonal antibody (MAb) B72.3, containing the murine variable region and a human gamma-1 constant region [designated cB72.3(gamma-1)]. cB72.3(gamma-1) was generated by first isolating functionally rearranged V(H) and V(L) genes of B72.3 from partial genomic libraries in phage vectors. Construction of mouse-human chimeric heavy and light chain genes was performed by inserting restriction fragments carrying V(L) and V(H) regions of B72.3 into unique sites of expression vectors which contain sequences encoding constant regions of human kappa and gamma-1, respectively. The expression constructs were subsequently electroporated into SP2/0 cells. The transfected SP2/0 murine cell line has been shown to synthesize cB72.3(gamma-1) at a level of 10-20-mu-g/ml. Reciprocal competition radioimmunoassays demonstrated that cB72.3(gamma-1), a previously described cB72.3(gamma-4), and native B72.3 (designated nB72.3) competed similarly. A rat anti-idiotype MAb made against nB72.3 was shown to bind equally well to cB72.3(gamma-11) and to the nB72.3. Immunochemical studies of the nB72.3, cB72.3(gamma-4), and cB72.3(gamma-1) revealed slight differences in size among the three MAb forms on sodium dodecyl sulfate gels and revealed a higher isoelectric point for the cB72.3(gamma-1). Antibody-dependent cell-mediated cytotoxicity experiments using human lymphokine-activated killer effector cells indicated better tumor cell killing by the cB72.3(gamma-1) than the nB72.3 or cB72.3(gamma-4). Dual label studies of coinjected cB72.3(gamma-1) and nB72.3 revealed that both MAbs could efficiently localize human tumor xenografts in athymic mice. Pharmacokinetic studies, analyzing the blood clearance of cB72.3(gamma-1), cB72.3(gamma-4), and nB72.3 in mice, showed that the nB72.3 beta-phase of clearance was slower than that of the other MAb forms. However, when the pharmacokinetic patterns of these three MAb forms were analyzed in monkeys, the cB72.3(gamma-1) and the nB72.3 showed similar clearance curves, while the cB72.2(gamma-4) showed a much slower plasma clearance. In view of the binding properties of nB72.3 and its ability to localize a range of carcinomas in clinical trials, the studies reported here demonstrate that the cB72.3(gamma-1) may serve as a potentially useful diagnostic and/or therapeutic reagent. C1 NCI,TUMOR IMMUNOL & BIOL LAB,BLDG 10,RM 8B07,BETHESDA,MD 20892. CELLTECH LTD,READING,BERKS,ENGLAND. AMER CYANAMID CO,LEDERLE LABS,DIV MED RES,PEARL RIVER,NY 10965. NR 39 TC 36 Z9 36 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 1 PY 1991 VL 51 IS 1 BP 181 EP 189 PG 9 WC Oncology SC Oncology GA EQ683 UT WOS:A1991EQ68300031 PM 1988082 ER PT J AU RIMOLDI, D SRIKANTAN, V WILSON, VL BASSIN, RH SAMID, D AF RIMOLDI, D SRIKANTAN, V WILSON, VL BASSIN, RH SAMID, D TI INCREASED SENSITIVITY OF NONTUMORIGENIC FIBROBLASTS EXPRESSING RAS OR MYC ONCOGENES TO MALIGNANT TRANSFORMATION INDUCED BY 5-AZA-2'-DEOXYCYTIDINE SO CANCER RESEARCH LA English DT Article ID INTERFERON-INDUCED REVERTANTS; BRONCHIAL EPITHELIAL-CELLS; C3H 10T1/2 CELLS; DNA METHYLATION; MOUSE CELLS; HA-RAS; 5-AZACYTIDINE; DIFFERENTIATION; TUMOR; GENE AB The hypomethylating chemotherapeutic drug 5-aza-2'-deoxycytidine (5AzadC) has been shown to induce cell differentiation in some systems, while promoting neoplastic transformation in others. Using both in vitro and in vivo models, we have explored the relationship between oncogene expression and the susceptibility of cells to malignant transformation by 5AzadC. The study involved several nontumorigenic subclones of NIH3T3 fibroblasts, including cells transfected with deregulated c-myc, as well as phenotypic revertants expressing v-Ki-ras or long terminal repeat-activated c-Ha-ras. Transient 5AzadC treatment of the oncogene-bearing cell lines was associated with a rapid and efficient neoplastic transformation. In some cases, over 50% of the cell population exhibited loss of contact inhibition of growth within 1 week of treatment. The transformants were capable of forming s.c. tumors and experimental lung metastases in recipient nude mice. In contrast, 5AzadC failed to induce malignant properties in control 3T3 cultures transfected with the bacterial neo(r) gene; rather, treatment of these cells was associated with differentiation into adipocytes and myotubes. The differential response to 5AzadC was also observed in vivo, in mice first inoculated s.c. with the premalignant cells and then treated with 5AzadC 24 h later. In agreement with the in vitro model, tumor development in mice correlated with the presence of cells with activated ras or myc oncogenes. Cytidine analogs that do not inhibit DNA methylation (i.e., 6-azacytidine and 1-beta-D-arabinofuranosyl cytosine) had no effect on cell phenotype. The results indicate that exposure of cells to 5AzadC can lead to tumor progression both in vitro and in vivo and suggest that preexisting alterations in oncogene expression may facilitate the evolution of cancerous growth induced by hypomethylating agents. C1 UNIFORMED SERV UNIV HLTH SCI,DEPT PATHOL,BETHESDA,MD 20814. CHILDRENS HOSP,DEPT PATHOL,DENVER,CO 80218. NCI,TUMOR IMMUNOL & BIOL LAB,BETHESDA,MD 20892. RI Ain, Kenneth/A-5179-2012 OI Ain, Kenneth/0000-0002-2668-934X FU NCI NIH HHS [R01-CA37351] NR 45 TC 20 Z9 20 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 1 PY 1991 VL 51 IS 1 BP 324 EP 330 PG 7 WC Oncology SC Oncology GA EQ683 UT WOS:A1991EQ68300053 PM 1703037 ER PT J AU STONER, GD KAIGHN, ME REDDEL, RR RESAU, JH BOWMAN, D NAITO, Z MATSUKURA, N YOU, M GALATI, AJ HARRIS, CC AF STONER, GD KAIGHN, ME REDDEL, RR RESAU, JH BOWMAN, D NAITO, Z MATSUKURA, N YOU, M GALATI, AJ HARRIS, CC TI ESTABLISHMENT AND CHARACTERIZATION OF SV40 T-ANTIGEN IMMORTALIZED HUMAN ESOPHAGEAL EPITHELIAL-CELLS SO CANCER RESEARCH LA English DT Article ID STRONTIUM PHOSPHATE TRANSFECTION; HUMAN EPIDERMAL-KERATINOCYTES; EARLY REGION GENES; CLONAL GROWTH; NEOPLASTIC TRANSFORMATION; SIMIAN VIRUS-40; VNTR MARKERS; CANCER; DIFFERENTIATION; LINES AB Normal human esophageal autopsy tissue was explanted in serum-free medium. The epithelial outgrowths were subcultured and then transfected by strontium phosphate coprecipitation with plasmid pRSV-T consisting of the RSV-LTR promoter and the sequence encoding the simian virus 40 large T-antigen. The transfected cells, but not the sham-transfected controls, formed multilayered colonies within 3-4 weeks, after which the colonies were transferred and cell strains (HE-451 and HE-457) developed. Both cell strains grew exponentially for 8-10 weeks and then senesced. After a "crisis" of 6-8 months, growth resumed in isolated colonies. One line, HET-1A from HE-457, was developed and has now undergone more than 250 population doublings. This line has retained epithelial morphology, stains positively for cytokeratins and the simian virus 40 T-antigen gene by immunofluorescence, and has remained nontumorigenic in athymic, nude mice for more than 12 months. Karyotypic analysis by Giemsa banding has shown that HET-1A is hypodiploid (34-40 chromosomes). Growth factor studies have shown that HET-1A is stimulated by Ca2+, and inhibited by fetal bovine serum, transforming growth factor-beta-1, and transforming growth factor-beta-2. This serum-free immortalized esophageal cell system will be useful for investigating the action of putative esophageal carcinogens. C1 MED COLL OHIO,DEPT DENT,TOLEDO,OH 43699. UNIV MARYLAND,SCH MED,DEPT PATHOL,BALTIMORE,MD 21201. NCI,DIV CANC ETIOL,HUMAN CARCINOGENESIS,BETHESDA,MD 20892. RP STONER, GD (reprint author), MED COLL OHIO,DEPT PATHOL,POB 10008,TOLEDO,OH 43699, USA. RI Reddel, Roger/A-6635-2014 OI Reddel, Roger/0000-0002-6302-6107 NR 50 TC 134 Z9 134 U1 1 U2 3 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 1 PY 1991 VL 51 IS 1 BP 365 EP 371 PG 7 WC Oncology SC Oncology GA EQ683 UT WOS:A1991EQ68300060 PM 1703038 ER PT J AU LEVY, AT CIOCE, V SOBEL, ME GARBISA, S GRIGIONI, WF LIOTTA, LA STETLERSTEVENSON, WG AF LEVY, AT CIOCE, V SOBEL, ME GARBISA, S GRIGIONI, WF LIOTTA, LA STETLERSTEVENSON, WG TI INCREASED EXPRESSION OF THE MR 72,000 TYPE-IV COLLAGENASE IN HUMAN COLONIC ADENOCARCINOMA SO CANCER RESEARCH LA English DT Article ID CELLS; METASTASIS; CARCINOMA; ACTIVATION; DIVERSITY; INDUCTION; RAS AB Proteolytic enzymes, such as type IV collagenase, play an important role in tumor invasion and metastasis. To examine M(r) 72,000 type IV collagenase expression in human colon carcinoma, blot hybridizations of total RNA from 19 primary colon tumors were performed. These filters were probed with complementary DNA probes encoding the M(r) 72,000 type IV collagenase metalloenzyme. The results were expressed as the ratio of the messenger RNA (mRNA) levels in the tumor tissue to that in the adjacent normal mucosa (R). The level of the 3.1-kilobase type IV collagenase mRNA was higher in the primary tumor than in the normal adjacent colonic mucosa in 13 of 18 (72%) cases with a diagnosis of adenocarcinoma. These cases were divided into high expression (R, 4.50 to 29.34) and intermediate expression (R, 2.54 to 3.31) subgroups. Both groups showed statistically significant (P < 0.05) elevations when compared with the five cases showing the lowest levels of M(r) 72,000 type IV collagenase mRNA expression (low expression subgroup; R, 0.96 to 1.48). With this demonstrated elevation of M(r) 72,000 type IV collagenase mRNA in colorectal adenocarcinoma we examined concomitant expression at the protein level using immunohistochemical techniques. Immunohistochemical examination of 70 cases of colon tumors, including 30 benign adenomas, using anti-M(r) 72,000 type IV collagenase antibodies demonstrated a significant correlation with Duke's classification (P < 0.001). Our results suggest that enhanced expression of the M(r) 72,000 type IV collagenase enzyme may be a marker of human colorectal tumor invasiveness. C1 NCI,PATHOL LAB,TUMOR INVAS & METASTASIS SECT,BETHESDA,MD 20892. IST ISTOL & EMBRIOL,PADUA,ITALY. UNIV BOLOGNA,IST ANAT PATOL,I-40126 BOLOGNA,ITALY. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 33 TC 333 Z9 339 U1 1 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 1 PY 1991 VL 51 IS 1 BP 439 EP 444 PG 6 WC Oncology SC Oncology GA EQ683 UT WOS:A1991EQ68300070 PM 1846313 ER PT J AU STAHL, JA LEONE, A ROSENGARD, AM PORTER, L KING, CR STEEG, PS AF STAHL, JA LEONE, A ROSENGARD, AM PORTER, L KING, CR STEEG, PS TI IDENTIFICATION OF A 2ND HUMAN NM23 GENE, NM23-H2 SO CANCER RESEARCH LA English DT Note ID EXPRESSION; METASTASIS AB Reduced RNA and/or protein levels corresponding to the murine nm23-1 and human nm23-H1 complementary DNA clones have been correlated with high tumor metastatic potential in several rodent model systems and human breast carcinomas. We report the identification of a second human nm23 gene, designated nm23-H2. The pNM23-H2S complementary DNA clone predicted a M(r) 17,000 protein 88% identical to nm23-H1. nm23-H2 also shared a significant homology with nucleoside diphosphate kinases and a Drosophila developmental gene. Southern blots containing Bg/II-restricted genomic DNA, which exhibited an allelic restriction fragment length polymorphism for nm23-H1, contained nonallelic bands upon rehybridization to the nm23-H2 probe. Thus, nm23-H1 and nm23-H2 are distinct genes. Northern blot hybridization of nm23-H1- and nm23-H2-specific probes to breast tumors and cell lines indicated that nm23-H1 expression was reduced in high metastatic potential tumor cells to a greater extent than nm23-H2. The data indicate that existence of a family independently regulated nm23 genes. C1 NCI,PATHOL LAB,BETHESDA,MD 20892. MOLEC ONCOL INC,GAITHERSBURG,MD 20878. RI Leone, Alvaro/K-6410-2016 OI Leone, Alvaro/0000-0003-3815-9052 FU NCI NIH HHS [R43 CA50054-01A1] NR 17 TC 409 Z9 425 U1 1 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 1 PY 1991 VL 51 IS 1 BP 445 EP 449 PG 5 WC Oncology SC Oncology GA EQ683 UT WOS:A1991EQ68300071 PM 1988104 ER PT J AU OBRAMS, GI GRUFFERMAN, S AF OBRAMS, GI GRUFFERMAN, S TI EPIDEMIOLOGY OF HIV ASSOCIATED NON-HODGKIN LYMPHOMA SO CANCER SURVEYS LA English DT Article ID ACQUIRED IMMUNODEFICIENCY SYNDROME; IMMUNE-DEFICIENCY SYNDROME; EPSTEIN-BARR VIRUS; B-CELL LYMPHOMA; HOMOSEXUAL MEN; SYNDROME AIDS; BURKITTS-LYMPHOMA; MALIGNANT-LYMPHOMA; TEMPORAL TRENDS; RISK C1 UNIV PITTSBURGH,SCH MED,DEPT CLIN EPIDEMIOL & PREVENT MED,PITTSBURGH,PA 15213. RP OBRAMS, GI (reprint author), NCI,EPIDEMIOL & BIOSTAT PROGRAM,BETHESDA,MD 20892, USA. NR 71 TC 21 Z9 22 U1 0 U2 0 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0261-2429 J9 CANCER SURV JI Cancer Surv. PY 1991 VL 10 BP 91 EP 102 PG 12 WC Oncology SC Oncology GA HP482 UT WOS:A1991HP48200007 PM 1821326 ER PT J AU RABKIN, CS BLATTNER, WA AF RABKIN, CS BLATTNER, WA TI HIV-INFECTION AND CANCERS OTHER THAN NON-HODGKIN LYMPHOMA AND KAPOSIS-SARCOMA SO CANCER SURVEYS LA English DT Article ID ACQUIRED IMMUNODEFICIENCY SYNDROME; RENAL-TRANSPLANT RECIPIENTS; HOMOSEXUAL MEN; SAN-FRANCISCO; SYNDROME AIDS; RISK; DISEASE; VIRUS; PAPILLOMAVIRUS; ASSOCIATION RP RABKIN, CS (reprint author), NCI,VIRAL EPIDEMIOL SECT,BETHESDA,MD 20892, USA. NR 34 TC 30 Z9 31 U1 0 U2 0 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0261-2429 J9 CANCER SURV JI Cancer Surv. PY 1991 VL 10 BP 151 EP 160 PG 10 WC Oncology SC Oncology GA HP482 UT WOS:A1991HP48200011 PM 1821320 ER PT J AU BLAIR, PC POPP, JA BRYANTVARELA, BJ THOMPSON, MB AF BLAIR, PC POPP, JA BRYANTVARELA, BJ THOMPSON, MB TI PROMOTION OF HEPATOCELLULAR FOCI IN FEMALE RATS BY CHENODEOXYCHOLIC ACID SO CARCINOGENESIS LA English DT Article ID SERUM BILE-ACIDS; PROTEIN KINASE-C; HEPATOBILIARY DISEASE; LITHOCHOLIC ACID; ALTERED FOCI; LIVER; HEPATOCARCINOGENESIS; MECHANISM; DEOXYCHOLATE; CHOLESTASIS AB Chenodeoxycholic acid (CDC), a dihydroxylated primary bile acid, was evaluated for promotional activity in the liver of rats using a two-stage initiation-promotion model. CDC is a primary bile acid that can attain high concentrations in serum and liver during induced or naturally occurring hepatocellular disorders. Female Sprague-Dawley rats were injected once (i.p) with diethylnitrosamine (DEN, 150 mg/kg) or sterile physiologic saline (SAL, 0.85% NaCl). Two weeks later, rats in each group were placed into one of two subgroups and fed either NIH-31 mash (Control) or NIH-31 mash containing 0.5% CDC for a 10 week period. At the end of the feeding period, blood and liver samples were collected for determination of bile acid profiles and quantitation of hepatocellular foci respectively. Serum samples were analyzed for concentrations of individual bile acids using a HPLC method that utilizes a post-column enzymatic reaction and fluorescence detection. Liver slices from the left hepatic lobe were stained for foci positive for placental glutathione S-transferase. In serum, significant increases occurred in concentrations of all forms of CDC and were accompanied by mild, insignificant increases in lithocholic acid. Decreased serum concentrations occurred in all forms of cholic and deoxycholic acids. Analysis of liver sections revealed that rats treated with DEN-CDC had significant increases in numbers and volume of foci compared to those treated with DEN-Control. For rats in groups DEN-CDC and DEN-Control, the numbers of foci per square centimeter were 32 and 12; per cubic centimeter, 2221 and 937; and the per cent volume of foci, 1.487 and 0.385 respectively. In this study, CDC was a promoter of hepatocellular foci. Because concentrations of CDC in liver and serum increase in a variety of hepatobiliary disorders, the possibility that increases in endogenous concentrations can enhance the formation of hepatocellular foci is being explored. C1 NIEHS,POB 12233,RES TRIANGLE PK,NC 27709. CHEM IND INST TOXICOL,RES TRIANGLE PK,NC 27709. NR 42 TC 12 Z9 12 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD JAN PY 1991 VL 12 IS 1 BP 59 EP 63 DI 10.1093/carcin/12.1.59 PG 5 WC Oncology SC Oncology GA EU656 UT WOS:A1991EU65600011 PM 1988183 ER PT J AU HASEGAWA, M RODBARD, D KINOSHITA, Y AF HASEGAWA, M RODBARD, D KINOSHITA, Y TI TIMING OF THE CAROTID ARTERIAL SOUNDS IN NORMAL ADULT MEN - MEASUREMENT OF LEFT-VENTRICULAR EJECTION, PREEJECTION PERIOD AND PULSE TRANSMISSION TIME SO CARDIOLOGY LA English DT Article DE CAROTID ARTERIAL SOUNDS; PHONOCARDIOGRAPHY; SYSTOLIC TIME INTERVALS; PREEJECTION PERIOD; LEFT VENTRICULAR EJECTION PERIOD; ECHOCARDIOGRAPHY; CAROTID PULSE WAVE PLETHYSMOGRAPHY ID INTERVALS AB The first and second carotid arterial sounds (CaS1 and CaS2) were recorded simultaneously with the aortic valve echocardiogram, carotid pulse wave contour, heart sounds, and electrocardiogram in 27 healthy, normal subjects. The mean intervals between the onset of the QRS complex and the onsets of the first and second major components of the carotid arterial sounds were Q-CaS1 = 131 +/- 15 ms and Q-CaS2 = 412 +/- 36 ms, respectively. The mean delay of CaS1 after aortic valve opening was 43 +/- 6 ms, while the delay of CaS2 after aortic valve closure was 43 +/- 7 ms. The onset of CaS1 and CaS2 are exactly coincident with the upstroke and with the dicrotic notch of the carotid pulse wave contour. The recording of the carotid arterial sounds, heart sounds, and ECG has enabled us to measure systolic time intervals including the pre-ejection period, left ventricular ejection time, and pulse transmission time more easily than using the conventional method involving the carotid arterial pulse wave contour. The new approach provides accuracy and precision comparable to that of the previous methods. C1 NICHHD,CTR CLIN,THEORET & PHYS BIOL LAB,ROOM 6C101,BETHESDA,MD 20892. CHIBA UNIV,HLTH SCI CTR,CHIBA,JAPAN. NR 15 TC 5 Z9 5 U1 0 U2 5 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0008-6312 J9 CARDIOLOGY JI Cardiology PY 1991 VL 78 IS 2 BP 138 EP 149 DI 10.1159/000174778 PG 12 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA FK741 UT WOS:A1991FK74100008 PM 2070371 ER PT J AU DOPPMAN, JL AF DOPPMAN, JL TI PERCUTANEOUS ASPIRATION FOR HORMONE LEVELS IN THE DIAGNOSIS OF FUNCTIONING ENDOCRINE TUMORS SO CARDIOVASCULAR AND INTERVENTIONAL RADIOLOGY LA English DT Article DE PERCUTANEOUS BIOPSY; ENDOCRINE TUMORS; HORMONE ASSAY AB Percutaneous aspiration to measure hormone levels in suspected functioning endocrine adenomas has been performed in 23 suspected cases of parathyroid adenomas, 7 cases of ACTH-producing thoracic carcinoid tumors, and 1 case of gastrinoma. When tumors secrete hormone or peptide markers, the biochemical assay can replace cytology to identify the lesion with greater specificity and at less expense. RP DOPPMAN, JL (reprint author), NIH,WARREN & MAGNUSON CLIN CTR,DEPT DIAGNOST RADIOL,BLDG 10,ROOM 1C660,BETHESDA,MD 20892, USA. NR 0 TC 5 Z9 5 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0174-1551 J9 CARDIOVASC INTER RAD JI Cardiovasc. Interv. Radiol. PD JAN-FEB PY 1991 VL 14 IS 1 BP 73 EP 77 DI 10.1007/BF02635535 PG 5 WC Cardiac & Cardiovascular Systems; Radiology, Nuclear Medicine & Medical Imaging SC Cardiovascular System & Cardiology; Radiology, Nuclear Medicine & Medical Imaging GA FA975 UT WOS:A1991FA97500013 PM 2044133 ER PT J AU DEMENT, JM AF DEMENT, JM TI CARCINOGENICITY OF CHRYSOTILE ASBESTOS - A CASE CONTROL STUDY OF TEXTILE WORKERS SO CELL BIOLOGY AND TOXICOLOGY LA English DT Article DE ASBESTOS; CHRYSOTILE; CANCER; TEXTILE WORKERS ID OCCUPATIONAL EPIDEMIOLOGY; VERMICULITE MINERS; MORTALITY; EXPOSURE; TREMOLITE; CONDUCT; DESIGN AB Chrysotile is the predominant type of asbestos used in the United States and thus represents the most important source of exposure to asbestos already in place. While the steepest exposure-response observed for lung cancer has been in workers exposed to chrysotile in textile operations, some argue that chrysotile is less carcinogenic than amphibole asbestos types. Mineral oil exposures have been hypothesized to be responsible for the highly elevated lung cancer risk seen in textile workers. A lung cancer case-control analysis among a cohort of South Carolina chrysotile asbestos textile workers was conducted. Only a modest reduction in the slope of the lung cancer exposure-response relationship was observed after controlling for mineral oil exposures. These data do not support mineral oil exposure as a plausible explanation for the elevated lung cancer risk seen in chrysotile asbestos textile workers. The possible role of longer, thinner, more carcinogenic fibers in textiles is one plausible hypothesis needing further investigation. RP DEMENT, JM (reprint author), NIEHS,111 ALEXANDER DR,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 19 TC 11 Z9 11 U1 1 U2 3 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0742-2091 J9 CELL BIOL TOXICOL JI Cell Biol. Toxicol. PD JAN PY 1991 VL 7 IS 1 BP 59 EP 65 DI 10.1007/BF00121330 PG 7 WC Cell Biology; Toxicology SC Cell Biology; Toxicology GA FA450 UT WOS:A1991FA45000005 PM 1647260 ER PT J AU HUFF, JE SALMON, AG HOOPER, NK ZEISE, L AF HUFF, JE SALMON, AG HOOPER, NK ZEISE, L TI LONG-TERM CARCINOGENESIS STUDIES ON 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN AND HEXACHLORODIBENZO-PARA-DIOXINS SO CELL BIOLOGY AND TOXICOLOGY LA English DT Article ID POLYCHLORINATED DIBENZOFURANS; TISSUE DISTRIBUTION; TUMOR PROMOTION; RISK ASSESSMENT; GUINEA-PIGS; AH LOCUS; RATS; MICE; MOUSE; RECEPTOR AB 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and 1,2,3,6,7,8- and 1,2,3,7,8,9-hexachlorodibenzo-p-dioxins (HCDDs) are among the most toxic and carcinogenic of "man-made" chemicals. These "dioxins," as well as many of the other polychlorinated dibenzodioxins (PCDDs) and dibenzofuran (PCDFs) derivatives, are chlorinated aromatic compounds which are chemically stable, insoluble in water, and highly soluble in fats and oils. TCDD acts as a complete carcinogen in several species, causing both common and uncommon tumors at multiple sites. It is a highly potent chemical carcinogen in chronic animal studies, producing carcinogenic effects in laboratory animals with doses as low as 0.001-mu-g/kg/day. In rats, TCDD induces neoplasms in the lung, oral/nasal cavities, thyroid and adrenal glands, and liver. In mice, TCDD induces neoplasms in the liver and subcutaneous tissue, thyroid gland, and thymic lymphomas. In hamsters, it induces squamous cell carcinomas of the facial skin. Tumors of the integumentary system are reported after oral (mice and rats), intraperitoneal (hamsters), and dermal (mice) administration. A mixture of HCDDS (defined as the mixture of the 1,2,3,6,7,8- and 1,2,3,7,8,9 isomers used in the NTP experiments) are potent liver carcinogens in mice and rats. Pharmacokinetic studies in laboratory animals indicate that 50-90% of dietary TCDD is absorbed. It concentrates in adipose tissue and the liver. In mammals, the TCDD present in the liver is slowly redistributed and stored in fatty tissue. Elimination of TCDD occurs via excretion of metabolites in the bile and urine and passively through the gut wall. Metabolism is slow: the biological half-life of TCDD varies from weeks (rodents) to years (humans), and is strongly dependent upon the rate of TCDD metabolism. Many of the toxic effects of TCDD, including teratogenicity, may arise by receptor-mediated mechanisms. The induction of cytochrome P-448 and related enzymes by TCDD occurs by such a mechanism, and is related to the binding of TCDD to the Ah receptor. The specific mechanism(s) by which TCDD exerts its carcinogenic effects is unclear: receptor-binding may be part of the story. The role of the Ah receptor has been indicated in a skin promotion assay. The evidence for mutagenicity is inconclusive. TCDD did not induce lethal mutations, chromosomal aberrations, micronuclei or sister chromotid exchanges in rodents treated in vivo, nor was it mutagenic to bacteria, but it did enhance transformation of mouse C3H 10T1/2 cells by N-methyl-N'-nitro-N-nitrosoguanidine and was mutagenic to mouse lymphoma cells. Under the State of California's Safe Drinking Water and Toxic Enforcement Act of 1986 ("Proposition 65"), TCDD and HCDDs were listed as chemicals known to the state to cause cancer. The International Agency for Research on Cancer (IARC) has classified TCDD as a Group 2B carcinogen ("possibly carcinogenic to humans"). Similarly, the EPA classified TCDD and HCDDs as Group B2 carcinogens ("probable human carcinogens"); and the DHHS' National Toxicology Program placed TCDD into the category of "substances which may reasonably be anticipated to be carcinogens for humans." C1 CALIF DEPT HLTH SERV,REPROD & CANC HAZARD ASSESSMENT SECT,BERKELEY,CA 94704. RP HUFF, JE (reprint author), NIEHS,RES TRIANGLE PK,NC 27709, USA. NR 81 TC 83 Z9 85 U1 0 U2 6 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0742-2091 J9 CELL BIOL TOXICOL JI Cell Biol. Toxicol. PD JAN PY 1991 VL 7 IS 1 BP 67 EP 94 DI 10.1007/BF00121331 PG 28 WC Cell Biology; Toxicology SC Cell Biology; Toxicology GA FA450 UT WOS:A1991FA45000006 PM 2054688 ER PT J AU TROEN, BR CHAUHAN, SS RAY, D GOTTESMAN, MM AF TROEN, BR CHAUHAN, SS RAY, D GOTTESMAN, MM TI DOWNSTREAM SEQUENCES MEDIATE INDUCTION OF THE MOUSE CATHEPSIN-L PROMOTER BY PHORBOL ESTERS SO CELL GROWTH & DIFFERENTIATION LA English DT Article ID MAJOR EXCRETED PROTEIN; TRANSCRIPTION INVITRO; STRUCTURAL PROTEINS; MUSCULAR-DYSTROPHY; ACID-PROTEASE; GROWTH-FACTOR; CYCLIC-AMP; GENE; CELLS; TRANSFORMATION AB The major excreted protein (MEP) of mouse fibroblasts is the precursor to a lysosomal acid protease (cathepsin L) whose synthesis is induced by malignant transformation, growth factors, tumor promoters, and cyclic AMP. We have previously cloned a functional gene for MEP from NIH 3T3 cells. When subcloned into chloramphenicol acetyl transferase (CAT) expression vectors, both 4-kilobase and 300 base pair fragments in the 5'-flanking region of the MEP gene confer CAT activity that is stimulated by cyclic AMP treatment but is not stimulated by phorbol ester treatment of NIH 3T3 cells. These fragments confer constitutive promoter activity that is comparable to that of the SV40 promoter. Primer extension, using RNA from cells transiently transfected with MEP-CAT fusion plasmids, demonstrates that phorbol ester treatment increases the amount of transcript from constructs containing both the promoter and sequences downstream of the transcription initiation site, including the first three introns, but not from constructs containing only the 5'-flanking region of the MEP gene. Nuclear run-off experiments confirm that the increase in endogenous MEP mRNA is mediated by increased transcription and not via relief of transcriptional attenuation. Since both the MEP promoter, which contains three potential binding sites for the AP-2 transcription factor, and the SV40 promoter, which contains both AP-1 and AP-2 binding sites, fail to respond to 12-O-tetradecanoylphorbol-13-acetate in NIH 3T3 cells, these upstream motifs are not sufficient to confer phorbol ester responsiveness in NIH 3T3 cells. These results suggest that the MEP gene is regulated in a complex manner by sequences both upstream and downstream of the transcription initiation site. C1 NCI,CELL BIOL LAB,BLDG 37,ROOM 2E18,BETHESDA,MD 20892. UNIV MICHIGAN,VET ADM MED CTR,CTR EDUC & CLIN,INST GERONTOL GERIATR RES,ANN ARBOR,MI 48109. NR 39 TC 32 Z9 32 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 1044-9523 J9 CELL GROWTH DIFFER JI Cell Growth Differ. PD JAN PY 1991 VL 2 IS 1 BP 23 EP 31 PG 9 WC Cell Biology SC Cell Biology GA ET575 UT WOS:A1991ET57500004 PM 1848774 ER PT J AU WESSELS, D MURRAY, J JUNG, G HAMMER, JA SOLL, DR AF WESSELS, D MURRAY, J JUNG, G HAMMER, JA SOLL, DR TI MYOSIN IB NULL MUTANTS OF DICTYOSTELIUM EXHIBIT ABNORMALITIES IN MOTILITY SO CELL MOTILITY AND THE CYTOSKELETON LA English DT Article DE DMIB- CELLS; F-ACTIN; CAMP ID INTRACELLULAR PARTICLE MOVEMENT; HEAVY-CHAIN GENE; CELL MOTILITY; MONOCLONAL-ANTIBODIES; PSEUDOPOD FORMATION; ACTIN; DISCOIDEUM; ACANTHAMOEBA; LOCALIZATION; CHEMOTAXIS AB Cellular and intracellular motility are compared between normal Dictyostelium amoebae and amoebae lacking myosin IB (DMIB-). DMIB- cells generate elongated cell shapes, form particulate-free pseudopodia filled with F-actin, and exhibit an anterior bias in pseudopod extension in a fashion similar to normal amoebae. DMIB- cells also exhibit a normal response to the addition of the chemoattractant cAMP, including a depression in cellular and intracellular particle velocity, depolymerization of F-actin in pseudopodia, and a concomitant increase in cortical F-actin. DMIB- cells do, however, form lateral pseudopodia roughly three times as frequently as normal cells, turn more often, and exhibit depressed average instantaneous cell velocity. DMIB- cells also exhibit a decrease in the average instantaneous velocity of intracellular particle movement and an increase in the degree of randomness in particle direction. These findings indicate that if there is functional substitution for myosin IB by other myosin I isoforms, it is at best only partial, with myosin IB being necessary for maintenance of the normal rate and persistence of cellular translocation, suppression of lateral pseudopod formation and subsequent turning, rapid intracellular particle motility, and the normal anterograde bias of intracellular particle movement. Furthermore, it is likely that the behavioral abnormalities observed here for DMIB- cells underlie the delay in the onset of chemotactic aggregation, the increase in the time required to complete streaming, and the abnormalities in morphogenesis exhibited by DMIB- cells. C1 UNIV IOWA,DEPT BIOL,IOWA CITY,IA 52242. NHLBI,CELL BIOL LAB,BETHESDA,MD 20892. FU NICHD NIH HHS [HD18577] NR 42 TC 125 Z9 126 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0886-1544 J9 CELL MOTIL CYTOSKEL JI Cell Motil. Cytoskeleton PY 1991 VL 20 IS 4 BP 301 EP 315 DI 10.1002/cm.970200406 PG 15 WC Cell Biology SC Cell Biology GA GX760 UT WOS:A1991GX76000005 PM 1666340 ER PT B AU STRACKE, ML AZNAVOORIAN, SA BECKNER, ME LIOTTA, LA SCHIFFMANN, E AF STRACKE, ML AZNAVOORIAN, SA BECKNER, ME LIOTTA, LA SCHIFFMANN, E BE GOLDBERG, ID TI CELL MOTILITY, A PRINCIPAL REQUIREMENT FOR METASTASIS SO CELL MOTILITY FACTORS SE EXPERIENTIA SUPPLEMENTA LA English DT Proceedings Paper CT INTERNATIONAL CONF ON CYTOKINES AND CELL MOTILITY CY MAY, 1990 CL NEW YORK, NY SP LONG ISLAND JEWISH MED CTR, NCI, LAB PATHOL RP STRACKE, ML (reprint author), NCI,PATHOL LAB,BETHESDA,MD 20892, USA. NR 0 TC 34 Z9 34 U1 0 U2 0 PU BIRKHAUSER VERLAG PI BASEL PA BASEL BN 3-7643-2569-0 J9 EXP SUPPL JI Exp. Suppl. PY 1991 VL 59 BP 147 EP 162 PG 16 WC Pathology SC Pathology GA BU19F UT WOS:A1991BU19F00010 PM 1833227 ER PT J AU HAGAMAN, JR AMBROSE, WW HIRSCH, PF KIEBZAK, GM AF HAGAMAN, JR AMBROSE, WW HIRSCH, PF KIEBZAK, GM TI AGE-RELATED-CHANGES IN RAT TRABECULAR, ENDOSTEAL, AND CORTICAL BONE DEMONSTRATED WITH SCANNING ELECTRON-MICROSCOPY SO CELLS AND MATERIALS LA English DT Article; Proceedings Paper CT SESSION ON THE AGED RAT MODEL FOR BONE BIOLOGY STUDIES, AT THE 1990 SCANNING MICROSCOPY MEETING CY MAY 07-09, 1990 CL BETHESDA, MD SP SCANNING MICROSCOPY INT DE AGING; BONE LOSS; SCANNING ELECTRON MICROSCOPY; SINGLE PHOTON ABSORPTIOMETRY; BACKSCATTERED ELECTRON IMAGING; RATS; EPIPHYSEAL PLATE; TRABECULAR BONE; ENDOSTEAL BONE; CORTICAL BONE ID PARATHYROID-HORMONE; MINERAL-CONTENT; FEMALE RATS; MODULATION; VERTEBRAE; SKELETON; DENSITY; MASS; SEM AB Scanning electron microscopy (SEM) was used in conjunction with light microscopy and single photon absorptiometry (SPA) to study age-related changes in femoral bone from Wistar-derived male and female rats 6, 12, and 24 months of age. SEM revealed progressive age-related loss of trabecular bone in the distal metaphysis in both male and female rats. Trabeculae extended further into the diaphysis in bones from females than from males, but receded from the diaphysis with increasing age in both sexes. The number of vascular channels in both male and female rats decreased with age, whereas the diameter of the channels increased markedly. Cortical bone from 24-month-old males, but not females, had numerous marrow-filled cavities. Light microscopy and SEM revealed irregular eroded surfaces, suggesting marked bone resorption on the endosteal surface of femurs from old rats of both sexes. A small loss of bone revealed by SPA is consistent with decreased trabecular and increased cortical bone that may serve as an architectural compensation to maintain bone strength with aging. Other age-related changes include increased medullary area, increased width of compact cortical bone at femoral midshaft, and epiphyseal closure in 24-month-old male rats. Our results and other studies demonstrate the usefulness of the rat in the study of age-related bone loss. C1 UNIV N CAROLINA,SCH DENT,DENT RES CTR,CHAPEL HILL,NC 27599. UNIV N CAROLINA,SCH MED,DEPT PHARMACOL,CHAPEL HILL,NC 27599. NIA,GERONTOL RES CTR,BIOL CHEM LAB,BALTIMORE,MD 21224. NR 27 TC 0 Z9 0 U1 1 U2 1 PU SCANNING MICROSCOPY INT PI CHICAGO PA PO BOX 66507, AMF O'HARE, CHICAGO, IL 60666 SN 1051-6794 J9 CELL MATER JI Cells Mat. PY 1991 SU 1 BP 37 EP 46 PG 10 WC Cell Biology; Medicine, Research & Experimental; Materials Science, Biomaterials SC Cell Biology; Research & Experimental Medicine; Materials Science GA HN949 UT WOS:A1991HN94900006 ER PT B AU LATHAM, PS PILARO, AM ECHEAGARY, PL VARESIO, LM AF LATHAM, PS PILARO, AM ECHEAGARY, PL VARESIO, LM BE WISSE, E KNOOK, DL MCCUSKEY, RS TI INTERLEUKIN-2 INDUCED CYTOTOXICITY OF RAT KUPFFER CELLS-INVITRO SO CELLS OF THE HEPATIC SINUSOID, VOL 3 LA English DT Proceedings Paper CT 5TH INTERNATIONAL SYMP ON CELLS OF THE HEPATIC SINUSOID CY AUG 26-30, 1990 CL TUCSON, AZ RP LATHAM, PS (reprint author), NCI,FREDERICK CANC RES & DEV CTR,MOLEC IMMUNOREGULAT LAB,BLDG 560 31-52,FREDERICK,MD 21702, USA. RI varesio, luigi/J-8261-2016 OI varesio, luigi/0000-0001-5659-2218 NR 0 TC 0 Z9 0 U1 0 U2 0 PU KUPFFER CELL FOUNDATION PI LEIDEN PA LEIDEN BN 90-800079-4-3 PY 1991 BP 312 EP 314 PG 3 WC Medicine, General & Internal; Pathology SC General & Internal Medicine; Pathology GA BU50X UT WOS:A1991BU50X00078 ER PT B AU ROTROSEN, D KLEINBERG, ME LETO, TL MALECH, HL AF ROTROSEN, D KLEINBERG, ME LETO, TL MALECH, HL BE MELTZER, MS MANTOVANI, A TI REGULATORY EVENTS IN ACTIVATION OF NADPH OXIDASE SO CELLULAR AND CYTOKINE NETWORKS IN TISSUE IMMUNITY SE PROGRESS IN LEUKOCYTE BIOLOGY LA English DT Proceedings Paper CT 27TH ANNUAL MEETING OF THE SOC FOR LEUKOCYTE BIOLOGY CY OCT 14-18, 1990 CL HERAKLION, GREECE SP SOC LEUKOCYTE BIOL RP ROTROSEN, D (reprint author), NIAID,CLIN INVEST LAB,BACTERIAL DIS SECT,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56097-9 J9 PROG LEUC B PY 1991 VL 11 BP 247 EP 252 PG 6 WC Immunology SC Immunology GA BU85E UT WOS:A1991BU85E00036 ER PT B AU COX, GW ESPINOZADELGADO, I LONGO, DL VARESIO, L AF COX, GW ESPINOZADELGADO, I LONGO, DL VARESIO, L BE MELTZER, MS MANTOVANI, A TI EXPRESSION AND FUNCTION OF INTERLEUKIN-2 RECEPTORS ON HUMAN MONOCYTES AND MURINE MACROPHAGES SO CELLULAR AND CYTOKINE NETWORKS IN TISSUE IMMUNITY SE PROGRESS IN LEUKOCYTE BIOLOGY LA English DT Proceedings Paper CT 27TH ANNUAL MEETING OF THE SOC FOR LEUKOCYTE BIOLOGY CY OCT 14-18, 1990 CL HERAKLION, GREECE SP SOC LEUKOCYTE BIOL RP COX, GW (reprint author), NCI,FREDERICK CANC RES & DEV CTR,BIOL RESPONSE MODIFIERS PROGRAM,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21702, USA. RI varesio, luigi/J-8261-2016 OI varesio, luigi/0000-0001-5659-2218 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56097-9 J9 PROG LEUC B PY 1991 VL 11 BP 301 EP 306 PG 6 WC Immunology SC Immunology GA BU85E UT WOS:A1991BU85E00045 ER PT J AU BROWN, CC MALECH, HL JACOBSON, RJ SHRIMPTON, CF BEVERLY, PC SEGAL, AW GALLIN, JI AF BROWN, CC MALECH, HL JACOBSON, RJ SHRIMPTON, CF BEVERLY, PC SEGAL, AW GALLIN, JI TI UNIQUE HUMAN NEUTROPHIL POPULATIONS ARE DEFINED BY MONOCLONAL-ANTIBODY ED12F8C10 SO CELLULAR IMMUNOLOGY LA English DT Article ID POLYMORPHONUCLEAR LEUKOCYTES; SUB-POPULATIONS; HETEROGENEITY; MONOCYTES; RESPONSIVENESS; IDENTIFICATION; SUBPOPULATIONS; COVERSLIPS; RECEPTORS; ENDOTOXIN C1 NIAID,CLIN INVEST LAB,BACTERIAL DIS SECT,BLDG 10,ROOM 11C103,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. GEORGETOWN UNIV,DEPT MED,WASHINGTON,DC 20057. UNIV LONDON UNIV COLL,RAYNE INST,DEPT MED,LONDON WC1E 6BT,ENGLAND. OI Segal, Anthony/0000-0001-7602-9043 NR 32 TC 9 Z9 9 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0008-8749 J9 CELL IMMUNOL JI Cell. Immunol. PD JAN PY 1991 VL 132 IS 1 BP 102 EP 114 DI 10.1016/0008-8749(91)90010-9 PG 13 WC Cell Biology; Immunology SC Cell Biology; Immunology GA EQ663 UT WOS:A1991EQ66300010 PM 1829650 ER PT J AU WEBER, TM JOOST, HG KURODA, M CUSHMAN, SW SIMPSON, IA AF WEBER, TM JOOST, HG KURODA, M CUSHMAN, SW SIMPSON, IA TI SUBCELLULAR-DISTRIBUTION AND PHOSPHORYLATION STATE OF INSULIN-RECEPTORS FROM INSULIN-TREATED AND ISOPROTERENOL-TREATED RAT ADIPOSE-CELLS SO CELLULAR SIGNALLING LA English DT Article DE INSULIN RECEPTORS; GLUCOSE TRANSPORT; CATECHOLAMINES; ISOPROTERENOL; PHOSPHORYLATION; ADIPOCYTE ID STIMULATED GLUCOSE-TRANSPORT; KINASE-ACTIVITY; PROTEIN-KINASE; ADIPOCYTES; CATECHOLAMINES; THEOPHYLLINE; ADENOSINE; INVITRO AB Rat adipose cells treated with insulin followed by isoproterenol exhibit a change in glucose transporter intrinsic activity (lowered maximal activity) and a decrease in insulin sensitivity (rightward shift of the concentration-response curve) when assayed for 3-O-methylglucose transport. To investigate the latter phenomenon, the distribution and phosphorylation state of insulin receptors was examined. Isoproterenol augmented the effect of insulin to reduce cell surface receptors by 20-30%. These receptors were recovered in microsomal fractions. Isoproterenol also markedly reduced insulin-stimulated [P-32]phosphate incorporation into the plasma membrane receptor beta-subunit. These effects may account for the effect of isoproterenol to decrease the sensitivity of the glucose transport response to insulin. RP WEBER, TM (reprint author), NIDDKD,EXPTL DIABET METAB & NUTR SECT,LBM,BLDG 10,ROOM 9B18,BETHESDA,MD 20892, USA. RI Joost, Hans-Georg/J-4462-2013 OI Joost, Hans-Georg/0000-0002-5860-606X NR 24 TC 7 Z9 7 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0898-6568 J9 CELL SIGNAL JI Cell. Signal. PY 1991 VL 3 IS 1 BP 51 EP 58 DI 10.1016/0898-6568(91)90007-H PG 8 WC Cell Biology SC Cell Biology GA FD397 UT WOS:A1991FD39700006 PM 2036296 ER PT J AU FARGIN, A YAMAMOTO, K COTECCHIA, S GOLDSMITH, PK SPIEGEL, AM LAPETINA, EG CARON, MG LEFKOWITZ, RJ AF FARGIN, A YAMAMOTO, K COTECCHIA, S GOLDSMITH, PK SPIEGEL, AM LAPETINA, EG CARON, MG LEFKOWITZ, RJ TI DUAL COUPLING OF THE CLONED 5-HT1A-RECEPTOR TO BOTH ADENYLYL CYCLASE AND PHOSPHOLIPASE-C IS MEDIATED VIA THE SAME GI-PROTEIN SO CELLULAR SIGNALLING LA English DT Article DE 5-HT1A-RECEPTOR; SIGNAL TRANSDUCTION; G1-PROTEINS; ADENYLYL CYCLASE; PHOSPHOLIPASE-C ID RECEPTORS; MEMBRANES; INHIBITION; HYDROLYSIS; NUCLEOTIDES; ACTIVATION; SUBSTRATE; SUBUNITS; AGONISTS; MANNER AB The cloned 5-HT1A receptor, stably expressed in HeLa cells, has been shown to mediate the effects of 5-hydroxytryptamine (5-HT) to inhibit cAMP formation and to stimulate the hydrolysis of phosphatidylinositol. Both responses were found to be pertussis toxin sensitive. We have examined these two responses in membranes derived from these cells and show that the 5-HT1A receptor can directly regulate the activity of adenylyl cyclase and phospholipase C in response to agonist. In order to examine whether the same or distinct guanine nucleotide-binding regulatory protein(s) (G protein) are involved in these two signal transduction pathways, we used anti-peptide antibodies recognizing the alpha-subunits of G(i1), G(i2), G(i3) as specific tools, since these pertussis toxin substrates are expressed in HeLa cells, These antibodies have previously been shown to prevent receptor-G protein coupling by binding to the regions of G proteins which are putatively involved in interaction with receptors. Our results indicate that the G(i) proteins, but preferentially G(i3), mediate the effects of 5-HT both to inhibit adenylyl cyclase and to stimulate phospholipase C. These findings demonstrate that the same receptor interacting with the same G protein can regulate several distinct effector molecules. C1 DUKE UNIV,MED CTR,HOWARD HUGHES MED INST,DEPT CELL BIOL,DURHAM,NC 27710. BURROUGHS WELLCOME CO,DIV CELL BIOL,RES TRIANGLE PK,NC 27709. DUKE UNIV,MED CTR,HOWARD HUGHES MED INST,DEPT BIOCHEM,DURHAM,NC 27710. NIDDKD,MOLEC PATHOPHYSIOL BRANCH,BETHESDA,MD 20892. RP FARGIN, A (reprint author), DUKE UNIV,MED CTR,HOWARD HUGHES MED INST,DEPT MED,DURHAM,NC 27710, USA. FU NHLBI NIH HHS [HL17670, HL16037] NR 31 TC 77 Z9 78 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0898-6568 J9 CELL SIGNAL JI Cell. Signal. PY 1991 VL 3 IS 6 BP 547 EP 557 DI 10.1016/0898-6568(91)90031-O PG 11 WC Cell Biology SC Cell Biology GA GY684 UT WOS:A1991GY68400006 PM 1786205 ER PT J AU REED, JC RAPP, U CUDDY, MP AF REED, JC RAPP, U CUDDY, MP TI TRANSFORMED 3T3-CELLS HAVE REDUCED LEVELS AND ALTERED SUBCELLULAR-DISTRIBUTION OF THE MAJOR PKC SUBSTRATE PROTEIN MARCKS SO CELLULAR SIGNALLING LA English DT Article DE PROTEIN KINASE-C; MARCKS; SUBSTRATE; KINASE; ONCOPROTEINS; SRC; RAS; BCL2 ID KINASE-C SUBSTRATE; DOWN-REGULATION; HUMAN-NEUTROPHILS; PHORBOL ESTERS; GROWTH-FACTORS; PHOSPHORYLATION; FIBROBLASTS; CELLS; DIACYLGLYCEROL; MYRISTOYLATION AB The MARCKS (myristylated alanine-rich C-kinase substrate) protein is an abundant calmodulin-binding protein that is a major and specific endogenous substrate of protein kinase C (PKC). Stimulation of cells with phorbol esters or other activators of PKC has been shown previously to result in rapid phosphorylation of MARCKS proteins and redistribution of these myristylated C-kinase substrates from membrane to cytosol. Here we show that NIH3T3 murine fibroblasts transformed by p21-HA-C-RAS or pp60-V-SRC oncoproteins have markedly reduced levels of p68-MARCKS and that most of the remaining MARCKS protein is found in the cytosol. 3T3 cells containing a nontransforming oncoprotein p26-BCL2, in contrast, exhibited normal levels and distribution of p68-MARCKS. When taken together with recent evidence that MARCKS proteins are involved in regulating organization of the membrane cytoskeleton, our findings suggest that oncoprotein-mediated alterations in MARCKS protein levels and subcellular distribution may contribute to the development or maintenance of the transformed phenotype. C1 NCI,RES FACIL,FREDERICK,MD 21701. RP REED, JC (reprint author), UNIV PENN,SCH MED,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104, USA. FU NCI NIH HHS [CA49576] NR 23 TC 22 Z9 22 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0898-6568 J9 CELL SIGNAL JI Cell. Signal. PY 1991 VL 3 IS 6 BP 569 EP 576 DI 10.1016/0898-6568(91)90033-Q PG 8 WC Cell Biology SC Cell Biology GA GY684 UT WOS:A1991GY68400008 PM 1838487 ER PT J AU SHIMIZU, Y SHAW, S AF SHIMIZU, Y SHAW, S TI LYMPHOCYTE ADHESION MEDIATED BY VLA (BETA-1) INTEGRINS - FUNCTIONAL ROLES OF MULTIPLE EXTRACELLULAR-MATRIX AND CELL-SURFACE LIGANDS SO CHEMICAL IMMUNOLOGY LA English DT Review ID PERIPHERAL-BLOOD LYMPHOCYTES; FIBRONECTIN RECEPTOR COMPLEX; MEMORY T-CELLS; ENDOTHELIAL-CELLS; LAMININ RECEPTOR; CYTOPLASMIC DOMAIN; COLLAGEN RECEPTORS; LEUKOCYTE ADHESION; HOMOTYPIC ADHESION; IDENTIFICATION C1 NCI, EXPTL IMMUNOL BRANCH, BETHESDA, MD 20892 USA. OI Shimizu, Yoji/0000-0001-9760-0288 NR 99 TC 10 Z9 10 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1015-0145 J9 CHEM IMMUNOL JI Chem. Immunol. PY 1991 VL 50 BP 34 EP 54 PG 21 WC Immunology SC Immunology GA GU406 UT WOS:A1991GU40600003 PM 1786106 ER PT J AU GLAUDEMANS, CPJ AF GLAUDEMANS, CPJ TI MAPPING OF SUBSITES OF MONOCLONAL, ANTI-CARBOHYDRATE ANTIBODIES USING DEOXY AND DEOXYFLUORO SUGARS SO CHEMICAL REVIEWS LA English DT Review ID BLOOD-GROUP DETERMINANT; MOUSE MYELOMA PROTEINS; PHOSPHORYLCHOLINE-CONTAINING ANTIGENS; HOMOGENEOUS MURINE IMMUNOGLOBULINS; NUCLEAR MAGNETIC-RESONANCE; STREPTOCOCCUS-PNEUMONIAE; MOLECULAR RECOGNITION; 3-DIMENSIONAL STRUCTURE; D-GALACTOPYRANOSIDES; NUCLEOTIDE-SEQUENCE RP GLAUDEMANS, CPJ (reprint author), NIH,BETHESDA,MD 20892, USA. NR 97 TC 105 Z9 105 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0009-2665 J9 CHEM REV JI Chem. Rev. PD JAN-FEB PY 1991 VL 91 IS 1 BP 25 EP 33 DI 10.1021/cr00001a002 PG 9 WC Chemistry, Multidisciplinary SC Chemistry GA EW662 UT WOS:A1991EW66200002 ER PT B AU GHANAYEM, BI MARONPOT, RR MATTHEWS, HB AF GHANAYEM, BI MARONPOT, RR MATTHEWS, HB BE BUTTERWORTH, BE SLAGA, TJ FARLAND, W MCCLAIN, M TI ROLE OF CHEMICALLY-INDUCED CELL-PROLIFERATION IN ETHYL ACRYLATE-INDUCED FORESTOMACH CARCINOGENESIS SO CHEMICALLY INDUCED CELL PROLIFERATION: IMPLICATIONS FOR RISK ASSESSMENT SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT CONF ON CHEMICALLY INDUCED CELL PROLIFERATION CY NOV 29-DEC 02, 1989 CL AUSTIN, TX SP HOFFMANN LA ROCHE, AMER IND HLTH, INT LIFE SCI INST, UNIV TEXAS, M D ANDERSON CANC CTR, SCI PARK, RES DIV, AMER PETR INST, DUPONT CO, US EPA, PROCTER & GAMBLE, ROHM & HAAS, SHELL OIL RP GHANAYEM, BI (reprint author), NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56111-8 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 369 BP 337 EP 346 PG 10 WC Toxicology SC Toxicology GA BU89W UT WOS:A1991BU89W00028 PM 1946529 ER PT B AU WARD, JM WEGHORST, CM DIWAN, BA KONISHI, N LUBET, RA HENNEMAN, JR DEVOR, DE AF WARD, JM WEGHORST, CM DIWAN, BA KONISHI, N LUBET, RA HENNEMAN, JR DEVOR, DE BE BUTTERWORTH, BE SLAGA, TJ FARLAND, W MCCLAIN, M TI EVALUATION OF CELL-PROLIFERATION IN THE KIDNEYS OF RODENTS WITH BROMODEOXYURIDINE IMMUNOHISTOCHEMISTRY OR TRITIATED-THYMIDINE AUTORADIOGRAPHY AFTER EXPOSURE TO RENAL TOXINS, TUMOR PROMOTERS, AND CARCINOGENS SO CHEMICALLY INDUCED CELL PROLIFERATION: IMPLICATIONS FOR RISK ASSESSMENT SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT CONF ON CHEMICALLY INDUCED CELL PROLIFERATION CY NOV 29-DEC 02, 1989 CL AUSTIN, TX SP HOFFMANN LA ROCHE, AMER IND HLTH, INT LIFE SCI INST, UNIV TEXAS, M D ANDERSON CANC CTR, SCI PARK, RES DIV, AMER PETR INST, DUPONT CO, US EPA, PROCTER & GAMBLE, ROHM & HAAS, SHELL OIL RP WARD, JM (reprint author), NCI,FREDERICK CANC RES & DEV CTR,TUMOR PATHOL & PATHOGENESIS SECT,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74102] NR 0 TC 9 Z9 9 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56111-8 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 369 BP 369 EP 388 PG 20 WC Toxicology SC Toxicology GA BU89W UT WOS:A1991BU89W00031 PM 1946533 ER PT J AU LUDEKE, B SCHUBERT, M YAMADA, Y LIJINSKY, W KLEIHUES, P AF LUDEKE, B SCHUBERT, M YAMADA, Y LIJINSKY, W KLEIHUES, P TI DNA HYDROXYETHYLATION BY HYDROXYETHYLNITROSOUREAS IN RELATION TO THEIR ORGAN SPECIFIC CARCINOGENICITY IN RATS SO CHEMICO-BIOLOGICAL INTERACTIONS LA English DT Article ID METHYL-N-NITROSOUREA; CLARA CELL; TISSUES; ALKYLATION; NITROSODIMETHYLAMINE; ETHYLNITROSOUREA; O6-METHYLGUANINE; INDUCTION; REPAIR AB N-Hydroxyethylnitroso-N'-ethylurea (HEENU) and N-hydroxyethylnitroso-N'-chloroethylurea (HCNU) are two of the few nitrosoureas which induce hepatocellular tumours in rats without further treatment. In the present study we have investigated whether this is due to selectively elevated levels of DNA hydroxyethylation in the target tissue. Formation of the promutagenic base O6-hydroxyethyldeoxyguanosine (O6-HEdG) in various rat tissues was determined by immuno-slot-blot assay. After a single dose by gavage (0.36 mmol/kg body wt) of HEENU, initial levels of O6-HEdG in liver and brain were close to the detection limit of 1.5-mu-mol/mol deoxyguanosine. In liver, steady state concentrations of 3.5-mu-mol/mol were reached after 6 h and maintained for at least 18 h. In brain, O6-HEdG levels were 1.7-mu-mol/mol after 6 h and 3.0-mu-mol/mol after 24 h. In a second experiment, the formation of O6-HEdG was assessed in target and non-target tissues 6 h after a single dose by gavage (0.36 mmol/kg) of HEENU, HCNU or hydroxyethylnitrosourea (HENU), which is not hepatocarcinogenic. The extent of DNA hydroxyethylation was greatest with HENU in all tissues examined. Concentrations of O6-HEdG were highest in liver (37.2-mu-mol/mol), followed by kidney (23.3-mu-mol/mol), lung (18.9-mu-mol/mol), brain (6.8-mu-mol/mol) and testes (3.8-mu-mol/mol). With HEENU and HCNU, levels of 1.4-3.3-mu-mol O6-HEdG/mol dG were observed in all tissues. In vitro, the alkylation reactions for all three compounds were nearly complete within 6 h. On a molar basis, yields of O6-HEdG in vitro were similar for HENU and HCNU and 3.7 times lower for HEENU. This suggests that the in vivo reactions of the dialkylnitrosoureas are by pathways other than or in addition to those occurring in vitro. We conclude that the hepatocarcinogenicity of HCNU and HEENU cannot be explained on the basis of their reaction with cellular DNA. C1 UNIV ZURICH,DEPT PATHOL,CH-8006 ZURICH,SWITZERLAND. NCI,FREDERICK CANC RES FACIL,ABL RES PROGRAM,FREDERICK,MD 21702. FU NCI NIH HHS [N01-CO-74101] NR 28 TC 6 Z9 6 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0009-2797 J9 CHEM-BIOL INTERACT JI Chem.-Biol. Interact. PY 1991 VL 79 IS 2 BP 207 EP 216 DI 10.1016/0009-2797(91)90083-J PG 10 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology GA GE325 UT WOS:A1991GE32500007 PM 1884430 ER PT J AU SMITH, BJ CURTIS, JF ELING, TE AF SMITH, BJ CURTIS, JF ELING, TE TI BIOACTIVATION OF XENOBIOTICS BY PROSTAGLANDIN-H-SYNTHASE SO CHEMICO-BIOLOGICAL INTERACTIONS LA English DT Review DE PROSTAGLANDIN-H-SYNTHASE; XENOBIOTIC; OXIDATION; BIOACTIVATION ID DEPENDENT MUTAGENIC ACTIVATION; PEROXIDASE-MEDIATED OXIDATION; HETEROCYCLIC AROMATIC-AMINES; FREE-RADICAL INTERMEDIATE; INDUCED PULMONARY-DISEASE; BUTYLATED HYDROXYTOLUENE; HORSERADISH-PEROXIDASE; ENDOPEROXIDE SYNTHETASE; METABOLIC-ACTIVATION; CO-OXIDATION AB Prostaglandin H synthase (PHS) catalyzes the oxidation of arachidonic acid to prostaglandin H-2 in reactions which utilize two activities, a cyclooxygenase and a peroxidase. These enzymatic activities generate enzyme- and substrate-derived free radical intermediates which can oxidize xenobiotics to biologically reactive intermediates. As a consequence, in the presence of arachidonic acid or a peroxide source, PHS can bioactivate many chemical carcinogens to their ultimate mutagenic and carcinogenic forms. In general, PHS-dependent bioactivation is most important in extrahepatic tissues with low monooxygenase activity such as the urinary bladder, renal medulla, skin and lung. Mutagenicity assays are useful in the detection of compounds which are converted to genotoxic metabolites during PHS oxidation. In addition, the oxidation of xenobiotics by PHS often form metabolites or adducts to cellular macromolecules which are specific for peroxidase- or peroxyl radical-dependent reactions. These specific metabolites and/or adducts have served as biological markers of xenobiotic bioactivation by PHS in certain tissues. Evidence is presented which supports a role for PHS in the bioactivation of several polycyclic aromatic hydrocarbons and aromatic amines, two classes of carcinogens which induce extrahepatic neoplasia. It should be emphasized that the toxicities induced by PHS-dependent bioactivation of xenobiotics are not limited to carcinogenicity. Examples are given which demonstrate a role for PHS in pulmonary toxicity, teratogenicity, nephrotoxicity and myelotoxicity. RP SMITH, BJ (reprint author), NIEHS,MOLEC BIOPHYS LAB,EICOSANOID BIOCHEM SECT,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 99 TC 69 Z9 72 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0009-2797 J9 CHEM-BIOL INTERACT JI Chem.-Biol. Interact. PY 1991 VL 79 IS 3 BP 245 EP 264 DI 10.1016/0009-2797(91)90108-J PG 20 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology GA GH427 UT WOS:A1991GH42700001 PM 1913972 ER PT J AU DATTA, AK RIGGS, CW FIVASH, MJ KASPRZAK, KS AF DATTA, AK RIGGS, CW FIVASH, MJ KASPRZAK, KS TI MECHANISMS OF NICKEL CARCINOGENESIS - INTERACTION OF NI(II) WITH 2'-DEOXYNUCLEOSIDES AND 2'-DEOXYNUCLEOTIDES SO CHEMICO-BIOLOGICAL INTERACTIONS LA English DT Article DE NICKEL; NI(II); 2'-DEOXYNUCLEOSIDES; 2'-DEOXYNUCLEOTIDES; DNA BASES ID BINDING; INVITRO; METALS; SITE; ION AB Interactions of Ni(II) with the base moieties of 2'-deoxynucleosides and 2'-deoxynucleotides were studied by means of UV difference spectroscopy in order to elucidate the mechanisms of site-specific enhancement by Ni(II) of DNA base oxidation with active oxygen species, observed previously (Kasprzak et al., Cancer Res., 49 (1989) 5964; Carcinogenesis, 11 (1990) 647). The interactions were generally weak and could be quantitated only at pH 7.2-7.9. The resulting coordination binding of Ni(II) was stronger with the purine derivatives, especially these of guanine, than with pyrimidine derivatives. Also, Ni(II) interacted more strongly with the bases of 2'-deoxynucleotides than with the bases of 2'-deoxynucleosides. The apparent stability constants for the interactions calculated with the use of a non-linear regression method, equalled 102 +/- 14, 159 +/- 30 and 290 +/- 70 M-1 for Ni(II) coordinated by 5'dAMP, 5'dADP and 5'dATP, respectively, and 305 +/- 73, 191 +/- 54, and 270 +/- 28 M-1 for 5'dGMP, 5'dGDP and 5'dGTP, respectively. Stability constant for the dG Ni(II) interaction was 39 +/- 7 M-1. Interactions of Ni(II) with the bases of dA, dC, dT and the dC- and dT- mono-, di- and tri-phosphates were too weak for meaningful quantitation. The strongest relative Ni(II) interaction with dG may explain high sensitivity of the dG site at the DNA molecule to Ni(II)-mediated oxidation observed in vitro and in vivo. The present results contrast with Ni(II)-directed site specific cleavage of DNA with H2O2 that occurs preferentially at the pyrimidine bases (Kawanishi et al., Carcinogenesis, 10 (1989) 2231). C1 NCI,FCRDC,DATA MANAGEMENT SERV,FREDERICK,MD 21702. RP DATTA, AK (reprint author), NCI,FCRDC,COMPARAT CARCINOGENESIS LAB,BLDG 538,RM 205E,FREDERICK,MD 21702, USA. NR 35 TC 18 Z9 18 U1 0 U2 5 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0009-2797 J9 CHEM-BIOL INTERACT JI Chem.-Biol. Interact. PY 1991 VL 79 IS 3 BP 323 EP 334 DI 10.1016/0009-2797(91)90112-K PG 12 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology GA GH427 UT WOS:A1991GH42700005 PM 1913976 ER PT J AU STOLZE, K MASON, RP AF STOLZE, K MASON, RP TI ESR SPECTROSCOPY OF FLOW-ORIENTED CATION RADICALS OF PHENOTHIAZINE-DERIVATIVES AND PHENOXATHIIN INTERCALATED IN DNA SO CHEMICO-BIOLOGICAL INTERACTIONS LA English DT Article DE ELECTRON SPIN RESONANCE; DNA; PHENOTHIAZINE; PHENOXATHIIN; FREE RADICALS; INTERCALATION ID ELECTRON-SPIN-RESONANCE; BIOLOGICAL MACROMOLECULES; IRREVERSIBLE BINDING; CHLORPROMAZINE AB Several derivatives of the phenothiazine cation radicals intercalated into DNA have been investigated using a new flow orientation technique. The anistropic hyperfine coupling constants of both the parallel and the perpendicular orientation relative to the magnetic field were measured and compared to previous results, which used different techniques. The phenoxathiin cation radical could also be stabilized by intercalation into DNA at pH 4, but the orientation technique revealed no further information due to the poor resolution of the experimental spectra. C1 NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709. NR 8 TC 16 Z9 16 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0009-2797 J9 CHEM-BIOL INTERACT JI Chem.-Biol. Interact. PY 1991 VL 77 IS 3 BP 283 EP 289 DI 10.1016/0009-2797(91)90037-8 PG 7 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology GA FF501 UT WOS:A1991FF50100003 PM 1849047 ER PT J AU HUANG, LQ MOORE, C MCGOWN, S TOMER, KB AF HUANG, LQ MOORE, C MCGOWN, S TOMER, KB TI THE APPLICATION OF HYBRID MASS-SPECTROMETRY MASS-SPECTROMETRY AND HIGH-RESOLUTION MASS-SPECTROMETRY TO THE ANALYSIS OF FISH SAMPLES FOR POLYCHLORINATED DIBENZO-P-DIOXINS AND DIBENZOFURANS SO CHEMOSPHERE LA English DT Article; Proceedings Paper CT 10TH INTERNATIONAL SYMP ON CHLORINATED DIOXINS AND RELATED COMPOUNDS CY SEP 10-14, 1990 CL BAYREUTH, GERMANY ID GAS-CHROMATOGRAPHY; MS-MS; CHLORINATED DIOXINS; GC-MS; 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN; ISOMERS AB A hybrid mass spectrometer operated in low resolution selected decomposition monitoring (SDM) mode, was used for the analysis of whole fish samples for the 2,3,7,8-substituted PCDD/F's. The hybrid MS/MS method using loss of COCl, gave similar quantitation results to those obtained by HRMS and eliminated the interferences by PCB's that were encountered in the HRMS analysis. C1 KRATOS ANALYT INC,RAMSEY,NJ 07446. NIEHS,RES TRIANGLE PK,NC 27709. RP HUANG, LQ (reprint author), CONNECTICUT AGR EXPT STN,BOX 1106,NEW HAVEN,CT 06504, USA. RI Tomer, Kenneth/E-8018-2013 NR 30 TC 2 Z9 2 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0045-6535 J9 CHEMOSPHERE JI Chemosphere PY 1991 VL 23 IS 8-10 BP 1043 EP 1053 DI 10.1016/0045-6535(91)90130-6 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA HB104 UT WOS:A1991HB10400008 ER PT J AU CLARK, G TRITSCHER, A LUCIER, G TAYLOR, M AF CLARK, G TRITSCHER, A LUCIER, G TAYLOR, M TI DOSE-DEPENDENT INCREASE IN TUMOR-NECROSIS-FACTOR-ALPHA PRODUCTION IN TCDD EXPOSED MICE IS AH RECEPTOR DEPENDENT SO CHEMOSPHERE LA English DT Article ID ENDOTOXIN HYPERSENSITIVITY; PERFLUORODECANOIC ACID; ACUTE TOXICITY; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN; SUPPRESSION; RESISTANCE; DIFFERS; LOCUS AB 2,3,7,8,-Tetrachlorodibenzo-p-dioxin (TCDD) treatment of C57BL/6J (Ah(bb)) mice resulted in a dose-dependent increase in TNF-alpha released into serum following endotoxin exposure, with a significant increase being observed at a dose of 10-mu-g/kg TCDD. At a dose of 500-mu-g/kg TCDD Ah(bb) mice demonstrated a 46-fold increase over the control response. In contrast, a congenic Ah receptor deficient mouse (Ah(dd)), did not show a significant increase in TNF-alpha production until exposed to 150-mu-g/kg TCDD and the maximum response was an 8-fold increase over control. TCDD exposure followed by endotoxin injection also resulted in a dose dependent increase in serum corticosterone levels that was not dependent on the Ah phenotype of the mouse. TNF-alpha is an endogenous pyrogen, it is elicited by endotoxin. The pyrogenic response to endotoxin increased to a greater extent in TCDD-treated Ah(bb) mice. These data suggest that the Ah receptor mediates TCDD-induced increases in the production of TNF-alpha following endotoxin exposure, and that this increase may be responsible for endotoxin hypersensitivity in TCDD-treated animals. RP CLARK, G (reprint author), NIEHS,RES TRIANGLE PK,NC 27709, USA. NR 14 TC 0 Z9 0 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0045-6535 J9 CHEMOSPHERE JI Chemosphere PY 1991 VL 23 IS 11-12 BP 1817 EP 1823 DI 10.1016/0045-6535(91)90029-D PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA HB255 UT WOS:A1991HB25500028 ER PT J AU DANNER, RL ELIN, RJ HOSSEINI, JM WESLEY, RA REILLY, JM PARILLO, JE AF DANNER, RL ELIN, RJ HOSSEINI, JM WESLEY, RA REILLY, JM PARILLO, JE TI ENDOTOXEMIA IN HUMAN SEPTIC SHOCK SO CHEST LA English DT Article ID RESPIRATORY-DISTRESS SYNDROME; NEGATIVE BACTERIAL SEPSIS; LIMULUS TEST; EARLY PREDICTOR; CANINE MODEL; ASSAY; BACTEREMIA; DYSFUNCTION; DEPRESSION; CHALLENGES AB To evaluate the incidence, pattern and clinical importance of endotoxemia in septic shock, frequent, serial endotoxin determinations were made prospectively in patients with shock. Detectable endotoxin occurred in 43 of 100 patients with septic shock, but in only one of ten patients with shock due to nonseptic causes. During septic shock, endotoxemia frequently occurred in the absence of Gram-negative bacteremia. Using a logistic regression model, multiple organ failure occurred 10.3 times more frequently and depression of left ventricular ejection fraction (less-than-or-equal-to 45 percent) 4.8 times more frequently in endotoxemic patients. In patients with positive blood cultures, endotoxemia was associated with a high mortality. We conclude that endotoxemia occurs frequently in septic shock and is associated with severe manifestations of this syndrome, including cardiac depression and multiple organ failure. This study suggests that endotoxin is an important mediator of septic shock and supports efforts to develop anti-endotoxin therapies for treating patients with this disease. C1 NIH,WARREN G MAGNUSON CLIN CTR,DEPT CLIN PATHOL,BETHESDA,MD 20892. NIH,WARREN G MAGNUSON CLIN CTR,DEPT INFORMAT SYST,BETHESDA,MD 20892. RP DANNER, RL (reprint author), NIH,WARREN G MAGNUSON CLIN CTR,DEPT CRIT CARE MED,BLDG 10,RM 10048,BETHESDA,MD 20892, USA. NR 35 TC 492 Z9 502 U1 1 U2 10 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD JAN PY 1991 VL 99 IS 1 BP 169 EP 175 DI 10.1378/chest.99.1.169 PG 7 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA EQ853 UT WOS:A1991EQ85300037 PM 1984950 ER PT J AU PUTNAM, FW AF PUTNAM, FW TI THE SATANIC RITUAL ABUSE CONTROVERSY SO CHILD ABUSE & NEGLECT LA English DT Editorial Material ID MULTIPLE PERSONALITY-DISORDER RP PUTNAM, FW (reprint author), NIMH,DEV PSYCHOL LAB,BLDG 15K,RM 105,9000 ROCKVILLE PIKE,BETHESDA,MD 20895, USA. NR 12 TC 33 Z9 33 U1 2 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0145-2134 J9 CHILD ABUSE NEGLECT JI Child Abuse Negl. PY 1991 VL 15 IS 3 BP 175 EP 179 DI 10.1016/0145-2134(91)90062-I PG 5 WC Family Studies; Psychology, Social; Social Work SC Family Studies; Psychology; Social Work GA FE424 UT WOS:A1991FE42400003 PM 2043969 ER PT J AU ONGKINGCO, JRC MANN, WA RULEY, EJ GREGG, RE AF ONGKINGCO, JRC MANN, WA RULEY, EJ GREGG, RE TI SEVERE HYPERLIPIDEMIA DUE TO MULTIPLE FACTORS IN A CHILD WITH NEPHROTIC SYNDROME SO CHILD NEPHROLOGY AND UROLOGY LA English DT Article DE HYPERLIPIDEMIA; HYPERLIPOPROTEINEMIA; NEPHROTIC SYNDROME; APOLIPOPROTEIN ID RENAL-TRANSPLANT RECIPIENTS; PLASMA; ATHEROSCLEROSIS; HYPERLIPEMIA; METABOLISM; ALBUMIN; THERAPY; LIPASE; LIPIDS; ADULT AB An unusual case of severe hypercholesterolemia and hypertriglyceridemia is described in a child with nephrotic syndrome. The severe hyperlipidemia in this patient was most likely induced by multiple interacting factors which included the metabolic abnormalities of nephrotic syndrome, steroid therapy, the underlying genetic predisposition of ApoE-2 homozygosity as well as diet and diuretic therapy. The result of these factors was an extremely severe type III hyperlipoproteinemia. The pathogenesis of hyperlipidemia in this setting is discussed. C1 GEORGE WASHINGTON UNIV,CHILDRENS NATL MED CTR,SCH MED,DEPT PEDIAT NEPHROL,WASHINGTON,DC 20052. GEORGE WASHINGTON UNIV,CHILDRENS NATL MED CTR,SCH MED,DEPT PEDIATR,WASHINGTON,DC 20052. NIH,MOLEC DIS BRANCH,BETHESDA,MD 20892. NR 29 TC 2 Z9 2 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1012-6694 J9 CHILD NEPHROL UROL JI Child Nephrol. Urol. PY 1991 VL 11 IS 2 BP 107 EP 110 PG 4 WC Pediatrics; Urology & Nephrology SC Pediatrics; Urology & Nephrology GA GG891 UT WOS:A1991GG89100012 PM 1756519 ER PT B AU SUOMI, SJ AF SUOMI, SJ BE BOCK, GR WHELAN, J TI EARLY STRESS AND ADULT EMOTIONAL REACTIVITY IN RHESUS-MONKEYS SO CHILDHOOD ENVIRONMENT AND ADULT DISEASE SE CIBA FOUNDATION SYMPOSIA LA English DT Proceedings Paper CT SYMP ON CHILDHOOD ENVIRONMENT AND ADULT DISEASE CY MAY 15-17, 1990 CL CIBA FDN, LONDON, ENGLAND HO CIBA FDN ID LONG-TERM ASSESSMENT; 10-YEAR PERSPECTIVE; SOCIAL RECOVERY; 1ST YEAR; LIFE; CHILDHOOD; BEHAVIOR RP SUOMI, SJ (reprint author), NICHHD,COMPARAT ETHOL LAB,BLDG 31,ROOM B2B15,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 0 TC 103 Z9 103 U1 0 U2 5 PU JOHN WILEY & SONS LTD PI CHICHESTER PA CHICHESTER BN 0-471-92957-3 J9 CIBA F SYMP PY 1991 VL 156 BP 171 EP 188 PG 18 WC Pathology; Pediatrics; Psychiatry SC Pathology; Pediatrics; Psychiatry GA BS94E UT WOS:A1991BS94E00011 PM 1855411 ER PT J AU HU, YL ZIFFER, H AF HU, YL ZIFFER, H TI A NEW MODEL TO ACCOUNT FOR THE ORDER IN WHICH ENANTIOMERS OF ALKYLARYLCARBINOLS ELUTE FROM A PIRKLE CHIRAL HPLC COLUMN - PREPARATION, ABSOLUTE STEREOCHEMISTRY, AND CHROMATOGRAPHIC PROPERTIES OF (+)-1,2-BENZOCYCLONONEN-3-OL AND (+)-1,2-BENZOCYCLODECEN-3-OL SO CHIRALITY LA English DT Article DE (R)-1,2-BENZOCYCLONONEN-3-OL; (S)-1,2-BENZOCYCLONONEN-3-OL; (R)-1,2-BENZOCYCLODECEN-3-OL; SEPARATION OF ENANTIOMERS OF PIRKLE HPLC COLUMNS; CHIRAL RECOGNITION ID RHIZOPUS-NIGRICANS; STATIONARY PHASES; CONFIGURATION; HYDROLYSES; CAMPHANATE; SEPARATION; RESOLUTION; ANALOGS AB Samples enriched in (-)- and (+)-1,2-benzocyclononen-3-ol were prepared by microbially mediated reactions. An enriched sample of (+)-1,2-benzocyclodecen-3-ol was prepared by fractional crystallization of the diastereoisomeric camphanates, followed by hydrolysis. The absolute stereochemistry of both alcohols was established by chemical transformations. The elution order of their enantiomers from a chiral Pirkle HPLC column [(R)-N-(3,5-dinitrobenzoyl) phenyl glycine ionically bound to gamma-aminopropyl silanized silica] was determined. The information in conjunction with other data was used to formulate a rule to predict the configuration of an enantiomer of an alkylarylcarbinol from its elution order from this column. C1 NATL INST DIABET & DIGEST & KIDNEY DIS,CHEM PHYS LAB,BETHESDA,MD 20892. NR 25 TC 3 Z9 3 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-0042 J9 CHIRALITY JI Chirality PY 1991 VL 3 IS 3 BP 196 EP 203 DI 10.1002/chir.530030310 PG 8 WC Chemistry, Medicinal; Chemistry, Analytical; Chemistry, Organic; Pharmacology & Pharmacy SC Pharmacology & Pharmacy; Chemistry GA FV016 UT WOS:A1991FV01600009 PM 1911049 ER PT J AU SOKOLOFF PORTER ROLAND WISE AF SOKOLOFF PORTER ROLAND WISE TI BRAIN ENERGY-METABOLISM - CELL BODY OR SYNAPSE - GENERAL DISCUSSION SO CIBA FOUNDATION SYMPOSIA LA English DT Article C1 MONASH UNIV, FAC MED, CLAYTON, VIC 3168, AUSTRALIA. KAROLINSKA HOSP, DEPT CLIN NEUROPHYSIOL, S-10401 STOCKHOLM 60, SWEDEN. WESTMINSTER & CHARING CROSS HOSP, PET CLIN GRP, LONDON W6 8RP, ENGLAND. RP NIMH, CEREBRAL METAB LAB, BLDG 36, ROOM 1A05, BETHESDA, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0300-5208 J9 CIBA F SYMP JI CIBA Found. Symp. PY 1991 VL 163 BP 43 EP 51 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA GZ543 UT WOS:A1991GZ54300004 ER PT J AU FOX SOKOLOFF FRACKOWIAK JONES RAICHLE LASSEN PLUM AF FOX SOKOLOFF FRACKOWIAK JONES RAICHLE LASSEN PLUM TI OXIDATIVE-METABOLISM IN BRAIN - GENERAL DISCUSSION SO CIBA FOUNDATION SYMPOSIA LA English DT Article C1 NIMH, CEREBRAL METAB LAB, BETHESDA, MD 20892 USA. HAMMERSMITH HOSP, MRC, CYCLOTRON UNIT, LONDON W12 0HS, ENGLAND. HAMMERSMITH HOSP, MRC, CYCLOTRON UNIT, PET METHODS SECT, LONDON W12 0HS, ENGLAND. WASHINGTON UNIV, SCH MED, EDWARD MALLINCKRODT INST RADIOL, DIV RADIAT SCI, ST LOUIS, MO 63110 USA. BISPEBJERG HOSP, DEPT CLIN PHYSIOL NUCL MED, DK-2400 COPENHAGEN, DENMARK. CORNELL UNIV, MED CTR, NEW YORK HOSP, DEPT NEUROL, NEW YORK, NY 10021 USA. RP UNIV TEXAS, HLTH SCI CTR, CTR RES IMAGING, 7703 FLOYD CURL DR, SAN ANTONIO, TX 78284 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0300-5208 J9 CIBA F SYMP JI CIBA Found. Symp. PY 1991 VL 163 BP 51 EP 56 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA GZ543 UT WOS:A1991GZ54300005 ER PT J AU BAX, A IKURA, M KAY, LE BARBATO, G SPERA, S AF BAX, A IKURA, M KAY, LE BARBATO, G SPERA, S TI MULTIDIMENSIONAL TRIPLE RESONANCE NMR-SPECTROSCOPY OF ISOTOPICALLY UNIFORMLY ENRICHED PROTEINS - A POWERFUL NEW STRATEGY FOR STRUCTURE DETERMINATION SO CIBA FOUNDATION SYMPOSIA LA English DT Article ID TWO-DIMENSIONAL NMR; STAPHYLOCOCCAL NUCLEASE; LARGER PROTEINS; 3-DIMENSIONAL STRUCTURE; SEQUENTIAL ASSIGNMENT; HETERONUCLEAR NMR; TRYPSIN-INHIBITOR; C-13; CALMODULIN; SPECTRA AB A procedure is described that affords complete H-1, C-13 and N-15 resonance assignment in proteins of up to about 25 kDa. The new approach requires uniform isotopic enrichment of the protein with C-13 and N-15 and correlates resonances of adjacent nuclei using the relatively large and well-resolved one-bond J couplings. Spectral overlap, a common problem in the application of two-dimensional NMR, is removed by increasing the dimensionality of the new methods to three or four, without increasing the number of observed resonances. With complete H-1, C-13 and N-15 resonance assignments available, the nuclear Overhauser effect (NOE)-based interproton distance constraints can be extracted in a very straightforward manner from four-dimensional NOE spectra. RP BAX, A (reprint author), NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892, USA. RI Barbato, Gaetano/G-4904-2011 NR 45 TC 24 Z9 24 U1 1 U2 1 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0300-5208 J9 CIBA F SYMP JI CIBA Found. Symp. PY 1991 VL 161 BP 108 EP 135 PG 28 WC Medicine, General & Internal SC General & Internal Medicine GA GP948 UT WOS:A1991GP94800008 PM 1814691 ER PT J AU SPORN, MB ROBERTS, AB AF SPORN, MB ROBERTS, AB TI WHAT IS TGF-BETA - INTRODUCTION SO CIBA FOUNDATION SYMPOSIA LA English DT Editorial Material ID TRANSFORMING GROWTH-FACTOR; PURIFICATION; CELLS; IDENTIFICATION; PROLIFERATION; SUPERFAMILY; INHIBITION; EXPRESSION; RECEPTORS; INVITRO RP SPORN, MB (reprint author), NCI,DIV CANC ETIOL,CHEMOPREVENT LAB,BLDG 41,ROOM C629,BETHESDA,MD 20892, USA. NR 34 TC 24 Z9 24 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0300-5208 J9 CIBA F SYMP JI CIBA Found. Symp. PY 1991 VL 157 BP 1 EP 6 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA GD851 UT WOS:A1991GD85100001 PM 2070680 ER PT J AU ROBERTS, AB KIM, SJ NOMA, T GLICK, AB LAFYATIS, R LECHLEIDER, R JAKOWLEW, SB GEISER, A OREILLY, MA DANIELPOUR, D SPORN, MB AF ROBERTS, AB KIM, SJ NOMA, T GLICK, AB LAFYATIS, R LECHLEIDER, R JAKOWLEW, SB GEISER, A OREILLY, MA DANIELPOUR, D SPORN, MB TI MULTIPLE FORMS OF TGF-BETA - DISTINCT PROMOTERS AND DIFFERENTIAL EXPRESSION SO CIBA FOUNDATION SYMPOSIA LA English DT Article ID TRANSFORMING GROWTH-FACTOR; VIRUS TYPE-1 TAX; FACTOR-BETA-1 TGF-BETA-1 GENE; STIMULATED MOUSE EPIDERMIS; MESSENGER-RNA EXPRESSION; PHORBOL ESTER OCCURS; HUMAN AQUEOUS-HUMOR; T-CELL LEUKEMIA; TRANSGENIC MICE; AUTOCRINE MECHANISMS AB There are now five known distinct isoforms of TGF-beta with 64-82% identity. Of these, only TGF-beta-1, 2 and 3 thus far have been demonstrated to be expressed in mammalian tissues; TGF-beta-4 has been described only in chicken and TGF-beta-5 only in frog. Although the biological activities of these five isoforms of TGF-beta are indistinguishable in most in vitro assays their sites of synthesis and localization in vivo are often distinct. Expression of the various isoforms is differentially controlled both in vivo, as in development, and in vitro after treatment of cells with steroids, such as oestrogen or tamoxifen, or with retinoids. To investigate the basis of these observations we have cloned and characterized the promoters for the human TGF-beta-1, 2 and 3 genes. Significant differences have been found: whereas the TGF-beta-1 promoter has no TATAA box and is regulated principally by AP-1 sites, both the TGF-beta-2 and 3 promoters have TATAA boxes as well as AP-2 sites and cAMP-responsive elements. Accordingly, TGF-beta-1 gene expression is induced strongly by phorbol esters whereas that of TGF-beta-2 and 3 is induced by forskolin, an activator of adenylate cyclase. Expression of TGF-beta-2 and 3 is often coordinately regulated in vivo in a pattern distinct from that of TGF-beta-1. RP ROBERTS, AB (reprint author), NCI,DIV CANC ETIOL,CHEMOPREVENT LAB,BLDG 41,ROOM C629,BETHESDA,MD 20892, USA. NR 47 TC 93 Z9 93 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0300-5208 J9 CIBA F SYMP JI CIBA Found. Symp. PY 1991 VL 157 BP 7 EP 28 PG 22 WC Medicine, General & Internal SC General & Internal Medicine GA GD851 UT WOS:A1991GD85100002 PM 1906395 ER PT J AU MARKS, F PALLADINO, MA MOSES, HL BALMAIN, A SPORN, MB FAUSTO, N RUSCETTI, FW DERYNCK, R AF MARKS, F PALLADINO, MA MOSES, HL BALMAIN, A SPORN, MB FAUSTO, N RUSCETTI, FW DERYNCK, R TI A STIMULATORY EFFECT OF TRANSFORMING GROWTH-FACTORS ON SKIN TUMOR-DEVELOPMENT INVIVO - DISCUSSION SO CIBA FOUNDATION SYMPOSIA LA English DT Discussion ID FACTOR-BETA; TRANSGENIC MICE; TGF-ALPHA; KERATINOCYTES; INDUCTION; CELLS; DIFFERENTIATION; OVEREXPRESSION; HYPERPLASIA; PROMOTION C1 GENENTECH INC,DEPT IMMUNOL RES & ASSAY TECHNOL,S SAN FRANCISCO,CA 94080. VANDERBILT UNIV,MED CTR,SCH MED,DEPT CELL BIOL,NASHVILLE,TN 37232. BEATSON INST CANC RES,WOLFSON LAB MOLEC PATHOL,GLASGOW G61 1BD,SCOTLAND. NCI,DIV CANC ETIOL,CHEMOPREVENT LAB,BETHESDA,MD 20892. BROWN UNIV,DEPT PATHOL & LAB MED,PROVIDENCE,RI 02912. NCI,FREDERICK CANC RES FACIL,BIOL RESPONSE MODIFIERS PROGRAM,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21701. GENENTECH INC,DEPT DEV BIOL,S SAN FRANCISCO,CA 94080. RP MARKS, F (reprint author), GERMAN CANC RES CTR,P 1011949,NEUENHEIMER FELD 280,W-6900 HEIDELBERG 1,GERMANY. NR 17 TC 0 Z9 0 U1 0 U2 1 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0300-5208 J9 CIBA F SYMP JI CIBA Found. Symp. PY 1991 VL 157 BP 130 EP 136 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA GD851 UT WOS:A1991GD85100010 ER PT J AU FAUSTO, N MEAD, JE GRUPPUSO, PA CASTILLA, A JAKOWLEW, SB AF FAUSTO, N MEAD, JE GRUPPUSO, PA CASTILLA, A JAKOWLEW, SB TI EFFECTS OF TGF-BETA-S IN THE LIVER - CELL-PROLIFERATION AND FIBROGENESIS SO CIBA FOUNDATION SYMPOSIA LA English DT Article ID TRANSFORMING GROWTH-FACTOR; RAT-LIVER; GENE-EXPRESSION; CARBON-TETRACHLORIDE; PARTIAL-HEPATECTOMY; EPITHELIAL-CELLS; FACTOR RECEPTORS; DNA-SYNTHESIS; INHIBITION; REGENERATION AB TGF-beta-1 is a potent inhibitor of hepatocyte proliferation in vivo and in culture and an inducer of fibrogenesis. It is produced by non-parenchymal cells in normal, regenerating, neoplastic and pre-neoplastic liver. TGF-beta-2 and beta-3 are also found in liver non-parenchymal cells and the amounts of their mRNAs increase during liver regeneration. TGF-beta-2 has similar effects to TGF-beta-1. Membranes from normal adult rat liver bind TGF-beta-1 with kinetics consistent with the presence of a single high affinity binding site; membranes from livers that have been regenerating for 12-72 hours show high affinity binding sites not detected in livers of normal or sham-operated rats. Affinity labelling of membranes from normal and regenerating liver shows two receptor proteins with M(r) 85 000 and 65 000. In contrast, a prominent band corresponding to a binding protein of M(r) 280 000 is detected in membrane preparations of cultured liver epithelial cells. Although modulation of TGF-beta-1 receptors occurs during liver regeneration, it has not been possible to determine which receptor is responsible for the TGF-beta-1 effects in hepatocytes. Other studies have demonstrated a significant correlation between TGF-beta-1 mRNA expression and various indicators of fibrogenesis in patients with chronic liver disease. Thus in animals and humans TGF-beta-1 appears to play a major role in the pathogenesis of fibrosis in chronic liver disease. C1 UNIV NAVARRA CLIN, DEPT INTERNAL MED, PAMPLONA, SPAIN. BROWN UNIV, DEPT PATHOL, PROVIDENCE, RI 02912 USA. BROWN UNIV, DEPT PEDIAT, PROVIDENCE, RI 02912 USA. NCI, CHEMOPREVENT LAB, BETHESDA, MD 20892 USA. FU NCI NIH HHS [CA 35249, CA 23226]; NICHD NIH HHS [HD 24455] NR 27 TC 42 Z9 43 U1 0 U2 4 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0300-5208 J9 CIBA F SYMP JI CIBA Found. Symp. PY 1991 VL 157 BP 165 EP 177 PG 13 WC Medicine, General & Internal SC General & Internal Medicine GA GD851 UT WOS:A1991GD85100013 PM 1649033 ER PT J AU RUSCETTI, FW DUBOIS, C FALK, LA JACOBSEN, SE SING, G LONGO, DL WILTROUT, RH KELLER, JR AF RUSCETTI, FW DUBOIS, C FALK, LA JACOBSEN, SE SING, G LONGO, DL WILTROUT, RH KELLER, JR TI INVIVO AND INVITRO EFFECTS OF TGF-BETA-1 ON NORMAL AND NEOPLASTIC HEMATOPOIESIS SO CIBA FOUNDATION SYMPOSIA LA English DT Article ID TRANSFORMING GROWTH-FACTOR; STEM-CELL PROLIFERATION; HEMATOPOIETIC PROGENITOR CELLS; NORMAL BONE-MARROW; FACTOR-BETA; 2 FORMS; INHIBITOR; FACTOR-BETA-1; LINES; IDENTIFICATION AB TGF-beta-1 and TGF-beta-2 are equipotent selective inhibitors of murine and human haemopoiesis in vitro. Primitive haemopoietic cells such as the high proliferative potential progenitor cell and the colony-forming unit (CFU)-GEMM are directly inhibited by TGF-beta whereas the more differentiated CFU-G, CFU-M and CFU-E are not. Recombinant TGF-beta-1 administered intraperitoneally or intravenously to mice selectively inhibits haemopoietic colony formation in a time-and dose-dependent manner to the same extent as seen in vitro. The progenitors are reversibly prevented from entering the cell cycle. This inhibitory action of TGF-beta functions on at least two levels: (1) down-modulation of the cell surface expression of receptors for growth stimulatory molecules and (2) interference with the intracellular signalling pathways of these molecules. In addition, expression of TGF-beta receptors is regulated during cytokine stimulation of haemopoiesis. Neoplastic B lymphocytes can proliferate by escaping from a TGF-beta-mediated autocrine inhibitory loop. Activation signals (e.g. phorbol esters) inhibit tumour cell growth by stimulating active TGF-beta production and inducing cell surface expression of TGF-beta receptors. These results indicate that TGF-beta may be useful as a bone marrow protective and/or an antitumour agent. C1 NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74102, 5R01CA27466]; NIADDK NIH HHS [5R01AM27424] NR 38 TC 22 Z9 22 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0300-5208 J9 CIBA F SYMP JI CIBA Found. Symp. PY 1991 VL 157 BP 212 EP 231 PG 20 WC Medicine, General & Internal SC General & Internal Medicine GA GD851 UT WOS:A1991GD85100016 PM 1649034 ER PT J AU SUOMI, SJ AF SUOMI, SJ TI EARLY STRESS AND ADULT EMOTIONAL REACTIVITY IN RHESUS-MONKEYS SO CIBA FOUNDATION SYMPOSIA LA English DT Article ID LONG-TERM ASSESSMENT; 10-YEAR PERSPECTIVE; SOCIAL RECOVERY; 1ST YEAR; LIFE; CHILDHOOD; BEHAVIOR AB This chapter examines the relationship between early social experiences and behavioural and emotional reactivity in adolescence and adulthood that has been established through extensive research with rhesus monkeys. Classic studies carried out in the 1960s first demonstrated that rearing under conditions of social isolation resulted in severe behavioural abnormalities that carried over into adulthood. In the 1970s techniques for reversing such isolation-induced deficits were developed. More recent studies have examined the long-term consequences of more subtle variation in early rearing environments. Monkeys reared from birth without mothers but with extensive peer contact develop relatively normal social behavioural repertoires and function well in familiar and stable social settings. However, peer-reared monkeys display extreme behavioural and physiological reactions to environmental challenges, such as brief social separation, later in life. In contrast, monkeys reared by unusually nurturant foster mothers appear to develop effective strategies for coping with subsequent environmental challenges. Some general principles that have emerged from these studies with rhesus monkeys will be outlined and their implications regarding possible relationships between early social experiences and responses to challenge later in life in humans will be discussed. RP SUOMI, SJ (reprint author), NICHHD,COMPARAT ETHOL LAB,BLDG 31,ROOM B2B15,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 35 TC 103 Z9 103 U1 0 U2 5 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0300-5208 J9 CIBA F SYMP JI CIBA Found. Symp. PY 1991 VL 156 BP 171 EP 188 PG 18 WC Medicine, General & Internal SC General & Internal Medicine GA FE857 UT WOS:A1991FE85700011 PM 1855411 ER PT J AU BONOW, RO DILSIZIAN, V CUOCOLO, A BACHARACH, SL AF BONOW, RO DILSIZIAN, V CUOCOLO, A BACHARACH, SL TI IDENTIFICATION OF VIABLE MYOCARDIUM IN PATIENTS WITH CHRONIC CORONARY-ARTERY DISEASE AND LEFT-VENTRICULAR DYSFUNCTION - COMPARISON OF THALLIUM SCINTIGRAPHY WITH REINJECTION AND PET IMAGING WITH F-18 FLUORODEOXYGLUCOSE SO CIRCULATION LA English DT Article DE CORONARY ARTERY DISEASE; FLUORODEOXYGLUCOSE; LEFT VENTRICULAR DYSFUNCTION; MYOCARDIAL VIABILITY; POSITRON EMISSION TOMOGRAPHY; TL-201 SCINTIGRAPHY ID POSITRON-EMISSION TOMOGRAPHY; BLOOD-FLOW; WALL-MOTION; HIBERNATING MYOCARDIUM; METABOLIC-ACTIVITY; F-18 DEOXYGLUCOSE; N-13 AMMONIA; PERFUSION; INFARCTION; (H2O)-O-15 AB In patients with chronic coronary artery disease and left ventricular dysfunction, the distinction between ventricular dysfunction arising from myocardial fibrosis and ischemic, but viable, myocardium has important clinical implications. By positron emission tomography (PET), enhanced fluorine-18-labeled fluorodeoxyglucose (FDG) uptake in myocardial segments with impaired function and reduced blood flow is evidence of myocardial viability. Reinjection of thallium-201 at rest immediately after stress-redistribution imaging may also provide evidence of myocardial viability by demonstrating thallium uptake in regions with apparently "irreversible" defects. To compare these two methods, we studied 16 patients with chronic coronary artery disease and left ventricular dysfunction (ejection fraction, 27 +/- 9%), all of whom had irreversible defects on standard exercise-redistribution thallium single-photon emission computed tomography (SPECT) imaging. Thallium was reinjected immediately after the redistribution study, and SPECT images were reacquired. The patients also underwent PET imaging with FDG and oxygen-15-labeled water. A total of 432 myocardial segments were analyzed from comparable transaxial tomograms, of which 166 (38%) had irreversible thallium defects on redistribution images before reinjection. FDG uptake was demonstrated in 121 (73%) of these irreversible defects. Irreversible defects were then subgrouped according to the degree of thallium activity, relative to peak activity in normal regions. Irreversible defects with only mild (60-85% of peak activity) or moderate (50-59% of peak) reduction in thallium activity were considered viable on the basis of FGD uptake in 91% and 84% of these segments, respectively. In contrast, in irreversible defects with severe reduction in thallium activity (< 50% of peak), FDG uptake was present in 51% of segments. In such severe defects, an identical number of segments (51%) demonstrated enhanced uptake of thallium after reinjection. In these severe "irreversible" defects, data on myocardial viability were concordant by the two techniques in 88% of segments, with 45% identified as viable and 43% identified as scar on both PET and thallium reinjection studies. These observations suggest that thallium imaging can be used to identify viable myocardium in patients with chronic coronary artery disease and left ventricular dysfunction. Most irreversible defects with only mild or moderate reduction in thallium activity represent viable myocardium as confirmed by FGD uptake. In myocardial regions with severe irreversible thallium defects on standard exercise-redistribution thallium imaging, thallium reinjection identifies as viable or nonviable, with a few exceptions, the same regions as does PET imaging with FDG. C1 NIH,CTR CLIN,DEPT NUCL MED,BETHESDA,MD 20892. RP BONOW, RO (reprint author), NHLBI,CARDIOL BRANCH,BLDG 10,ROOM 7B-15,BETHESDA,MD 20892, USA. OI Cuocolo, Alberto/0000-0003-3431-7658 NR 32 TC 523 Z9 533 U1 1 U2 5 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD JAN PY 1991 VL 83 IS 1 BP 26 EP 37 PG 12 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA ER026 UT WOS:A1991ER02600003 PM 1984883 ER PT J AU SCHATZ, RA BAIM, DS LEON, M ELLIS, SG GOLDBERG, S HIRSHFELD, JW CLEMAN, MW CABIN, HS WALKER, C STAGG, J BUCHBINDER, M TEIRSTEIN, PS TOPOL, EJ SAVAGE, M PEREZ, JA CURRY, RC WHITWORTH, H SOUSA, JE TIO, F ALMAGOR, Y PONDER, R PENN, IM LEONARD, B LEVINE, SL FISH, RD PALMAZ, JC AF SCHATZ, RA BAIM, DS LEON, M ELLIS, SG GOLDBERG, S HIRSHFELD, JW CLEMAN, MW CABIN, HS WALKER, C STAGG, J BUCHBINDER, M TEIRSTEIN, PS TOPOL, EJ SAVAGE, M PEREZ, JA CURRY, RC WHITWORTH, H SOUSA, JE TIO, F ALMAGOR, Y PONDER, R PENN, IM LEONARD, B LEVINE, SL FISH, RD PALMAZ, JC TI CLINICAL-EXPERIENCE WITH THE PALMAZ-SCHATZ CORONARY STENT - INITIAL RESULTS OF A MULTICENTER STUDY SO CIRCULATION LA English DT Article DE CORONARY ARTERY DISEASE; CORONARY ANGIOPLASTY; COMPLICATIONS; STENTS ID INTRAVASCULAR STENTS; ACUTE CLOSURE; FOLLOW-UP; ANGIOPLASTY; RESTENOSIS; ENDOPROSTHESES; OCCLUSION; REGISTRY; ARTERIES; GRAFTS AB Stenting of native coronary arteries with a balloon-expandable stent was attempted to 226 patients after elective angioplasty. Delivery of the device was successful in 213 (94%) of the patients. Of these, 39 received aspirin and dipyridamole only (group 1) and 174 received aspirin, dipyridamole, and warfarin for 1-3 months (group 2). There was no abrupt closure (less-than-or-equal-to 1 day) or perioperative death in either group. In-hospital or perioperative complications in group 1 compared with group 2 were as follows: subacute closure (1-14 days), seven (18%) patients versus one (0.6%) patient, respectively, p < 0.0001; myocardial infarction, five (13%) patients versus one (0.6%) patient, respectively. Thus, the incidence of major complications such as death, myocardial infarction, or a condition requiring urgent by pass surgery was 15% in group 1 and 0.6% in group 2. Clinical follow-up revealed that 92% of the patients were asymptomatic at 3 months after stenting compared with 6% before stenting (p < 0.0001). Of the 13 patients who were symptomatic, nine underwent cardiac catheterization and, ultimately, successful elective coronary angioplasty or bypass surgery. We conclude that a high delivery success rate can be expected with this device and the clinical thrombosis is less frequent in anticoagulated patients than in nonanticoagulated patients. Furthermore, in this selected patient population, coronary stenting results in a low incidence of in-hospital and perioperative complications. Clinical success, defined by absence of symptoms, appears to be sustained at 3 months. C1 ARIZONA HEART INST FDN,DIV CARDIOL,PHOENIX,AZ. BETH ISRAEL HOSP,BOSTON,MA 02215. NIH,BETHESDA,MD 20892. UNIV MICHIGAN,ANN ARBOR,MI 48109. JEFFERSON HOSP,PHILADELPHIA,PA. UNIV PENN,MED CTR,PHILADELPHIA,PA 19104. YALE HOSP,NEW HAVEN,CT. CARDIOVASC INST S,HOUMA,LA. UNIV CALIF SAN DIEGO,LA JOLLA,CA 92093. SCRIPPS MED CLIN,LA JOLLA,CA. FLORIDA HOSP,ORLANDO,FL. INST DANTE PAZZANESE CARDIOL ESTADO SAO PAULO,SAO PAULO,BRAZIL. UNIV TEXAS,DEPT PATHOL,SAN ANTONIO,TX 78285. UNIV TEXAS,DEPT CARDIOL,SAN ANTONIO,TX 78285. UNIV TEXAS,DEPT RADIOL,SAN ANTONIO,TX 78285. BRIGHAM & WOMENS HOSP,BOSTON,MA 02115. NR 38 TC 543 Z9 551 U1 1 U2 7 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD JAN PY 1991 VL 83 IS 1 BP 148 EP 161 PG 14 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA ER026 UT WOS:A1991ER02600016 PM 1984878 ER PT J AU PASSAMANI, ER AF PASSAMANI, ER TI UNSTABLE ANGINA - QUALITY-OF-LIFE SO CIRCULATION LA English DT Editorial Material ID SURGICAL-TREATMENT; STABLE ANGINA; ASPIRIN; SURVIVAL; PECTORIS; SURGERY; DISEASE; THERAPY; TRIAL RP PASSAMANI, ER (reprint author), NHLBI,BETHESDA,MD 20892, USA. NR 27 TC 3 Z9 3 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD JAN PY 1991 VL 83 IS 1 BP 342 EP 344 PG 3 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA ER026 UT WOS:A1991ER02600037 PM 1984893 ER PT J AU ROTH, BJ AF ROTH, BJ TI ACTION-POTENTIAL PROPAGATION IN A THICK STRAND OF CARDIAC-MUSCLE SO CIRCULATION RESEARCH LA English DT Article DE BIDOMAIN; PROPAGATION; ANISOTROPY; INTERSTITIAL SPACE ID DIMENSIONAL ANISOTROPIC BISYNCYTIA; PURKINJE-FIBERS; ELECTRICAL-ACTIVITY; EXTRACELLULAR POTENTIALS; DIRECTIONAL DIFFERENCES; DISCONTINUOUS NATURE; TRABECULAR MUSCLE; MAMMALIAN HEART; SODIUM CURRENT; MODEL AB A theoretical model of action potential propagation in a thick strand of cardiac muscle is presented. The calculation takes into account the anisotropic and syncytial properties of the tissue, the presence of the interstitial space, the effect of the surrounding tissue bath, and the variation of the potential both along the strand length and across the strand cross section. The bidomain model is used to represent the electrical properties of the tissue, and the Ebihara-Johnson model is used to represent the properties of the active sodium channels. The calculated wave front is curved, with the action potential at the surface of the strand leading that at the center. The rate of rise of the action potential and the time constant of the action potential foot vary with depth into the tissue. The velocity of the wave front is nearly independent of strand radius for radii greater than 0.5 mm. The conduction velocity decreases as the volume fraction of the interstitial space decreases. In the limit of tightly packed cells, an action potential propagates quickly over the surface of the strand; the bulk of the tissue is then excited by a slow inward wave front initiated on the surface. This model does not predict an increase in conduction velocity when cells are tightly packed, a hypothesis that has been proposed previously to explain the fast conduction velocity in Purkinje fibers of some species. RP ROTH, BJ (reprint author), NIH, NATL CTR RES RESOURCES, BIOMED ENGN & INSTRUMENTAT PROGRAM, BLDG 13, BETHESDA, MD 20892 USA. RI Roth, Bradley/A-4920-2008 NR 58 TC 106 Z9 107 U1 1 U2 4 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7330 J9 CIRC RES JI Circ.Res. PD JAN PY 1991 VL 68 IS 1 BP 162 EP 173 PG 12 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA ER288 UT WOS:A1991ER28800017 PM 1984858 ER PT B AU JOYCE, ME JINGUSHI, S SCULLY, SP BOLANDER, ME AF JOYCE, ME JINGUSHI, S SCULLY, SP BOLANDER, ME BE BARBUL, A CALDWELL, MD EAGLSTEIN, WH HUNT, TK MARSHALL, D PINES, E SKOVER, G TI ROLE OF GROWTH-FACTORS IN FRACTURE-HEALING SO CLINICAL AND EXPERIMENTAL APPROACHES TO DERMAL AND EPIDERMAL REPAIR: NORMAL AND CHRONIC WOUNDS SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT 3RD INTERNATIONAL CONF ON TISSUE REPAIR CY JAN 10-14, 1990 CL MIAMI, FL SP JOHNSON & JOHNSON MED RP JOYCE, ME (reprint author), NIAMS,ORTHOPAED RES UNIT,BETHESDA,MD, USA. NR 0 TC 78 Z9 82 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56075-8 J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 365 BP 391 EP 416 PG 26 WC Biochemistry & Molecular Biology; Dermatology; Surgery SC Biochemistry & Molecular Biology; Dermatology; Surgery GA BT24A UT WOS:A1991BT24A00031 PM 1862146 ER PT J AU SHIMIZU, S NARA, Y YAMORI, Y KEISER, HR AF SHIMIZU, S NARA, Y YAMORI, Y KEISER, HR TI DIFFERENCES IN RESPONSE BETWEEN SHRSP AND WKY VASCULAR SMOOTH-MUSCLE CELLS TO INHIBITION OF CELL-PROLIFERATION BY SODIUM-NITROPRUSSIDE SO CLINICAL AND EXPERIMENTAL HYPERTENSION PART A-THEORY AND PRACTICE LA English DT Article; Proceedings Paper CT 6TH INTERNATIONAL SYMP ON RATS WITH SPONTANEOUS HYPERTENSION AND RELATED STUDIES CY MAY 22-24, 1989 CL IOWA CITY, IA DE SHRSP; WKY; VASCILAR SMOOTH MUSCLE CELLS; CELL PROLIFERATION; SODIUM NITROPRUSSIDE ID SPONTANEOUSLY HYPERTENSIVE RATS; GROWTH-FACTOR; MECHANISMS; CA-2+ AB The effects of sodium nitroprusside on the proliferation of cultured vascular smooth muscle cells (SMC) from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto rats (WKY) were examined. Sodium-nitroprusside (SNP, 1mM) inhibited DNA synthesis by SMC from SHRSP and WKY by 90% and 95% , respectively. SNP also decreased cell proliferation. However, SNP was inactive when SMC were pretreated 6 and 12 hrs before growth stimulation by FCS. On the other hand, the inhibitory action of SNP decreased with time (posttreatment) in SHRSP SMC (42% inhibition at 12 hr post addition). However, in WKY SMC, the decrease of inhibitory action was very slight and SNP still inhibited 73% of DNA synthesis at 12 hr post treatment. SNP inhibited RNA synthesis for 3-6 hr and 6-9 hr after FCS stimulation, however no difference was noted between SHRSP and WKY. Protein synthesis was inhibited more strongly by SNP (9-12 hr after FCS stimulation) in WKY SMC than SHRSP SMC. It is probable that the specific protein(S) whose synthesis is inhibited by SNP is/are responsible for the differences in cell proliferation rate between SHRSP and WKY SMC. C1 SHIMANE MED UNIV,DEPT PATHOL,IZUMO,SHIMANE 693,JAPAN. RP SHIMIZU, S (reprint author), NIH,HYPERTENS ENDOCRINE BRANCH,BETHESDA,MD 20892, USA. NR 12 TC 3 Z9 3 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0730-0077 J9 CLIN EXP HYPERTENS A PY 1991 VL 13 IS 5 BP 787 EP 795 DI 10.3109/10641969109042081 PG 9 WC Cardiac & Cardiovascular Systems; Pharmacology & Pharmacy; Physiology SC Cardiovascular System & Cardiology; Pharmacology & Pharmacy; Physiology GA GM492 UT WOS:A1991GM49200017 PM 1773510 ER PT J AU MANTOVANI, V CORAZZA, GR ANGELINI, G DELFINO, L FRISONI, M MIRRI, P VALENTINI, RA BARBONI, P GASBARRINI, G FERRARA, GB AF MANTOVANI, V CORAZZA, GR ANGELINI, G DELFINO, L FRISONI, M MIRRI, P VALENTINI, RA BARBONI, P GASBARRINI, G FERRARA, GB TI MOLECULAR ANALYSIS OF HLA-DQ A ALLELES IN CELIAC-DISEASE LACK OF A UNIQUE DISEASE-ASSOCIATED SEQUENCE SO CLINICAL AND EXPERIMENTAL IMMUNOLOGY LA English DT Article DE HLA; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; POLYMERASE CHAIN REACTION; CELIAC DISEASE ID CELIAC-DISEASE; SUSCEPTIBILITY; ALPHA; DNA; HAPLOTYPE; HETERODIMER; ALLOTYPES; HLA-DR3; PROBES; GENE AB Susceptibility to coeliac disease is strongly associated with some HLA class II antigens, encoded by the HLA-D region. Since the HLA-DQ locus seems to be primarily involved, we have analysed by polymerase chain reaction amplification and allele-specific oligonucleotide hybridization the most polymorphic region of the HLA-DQ A1 gene. No difference was observed between the 20 coeliac patients and 20 HLA-D-matched healthy controls who took part in the study. Furthermore, in patients and controls, the restriction fragment length polymorphism analysis of the HLA-DQ A gene using the restriction enzyme Bg/II did not disclose any specific disease-associated fragment. Our results are not consistent with a unique DQ A coeliac disease-associated sequence, but rather with the hypothesis that some polymorphic residues or allelic hypervariable regions, although found also in the normal population, can predispose to coeliac disease due to their higher frequency in this condition. C1 UNIV BOLOGNA,POLICLIN S ORSOLA,DEPT MED PATHOL 1,I-40138 BOLOGNA,ITALY. MALPIGHI BOLOGNA HOSP,TISSUE TYPING LAB,BOLOGNA,ITALY. NATL CANC INST,GENOA,ITALY. RI Corazza, Gino Roberto/K-8500-2016 OI Corazza, Gino Roberto/0000-0001-9532-0573 NR 32 TC 10 Z9 10 U1 0 U2 1 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0009-9104 J9 CLIN EXP IMMUNOL JI Clin. Exp. Immunol. PD JAN PY 1991 VL 83 IS 1 BP 74 EP 78 PG 5 WC Immunology SC Immunology GA ER663 UT WOS:A1991ER66300014 PM 1671007 ER PT J AU GRANINGER, WB STEINBERG, AD MERON, G SMOLEN, JS AF GRANINGER, WB STEINBERG, AD MERON, G SMOLEN, JS TI INTERSTITIAL NEPHRITIS IN PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS - A MANIFESTATION OF CONCOMITANT SJOGRENS-SYNDROME SO CLINICAL AND EXPERIMENTAL RHEUMATOLOGY LA English DT Article DE INTERSTITIAL NEPHRITIS; SLE; SJOGRENS SYNDROME; RENAL TUBULAR ACIDOSIS ID RENAL TUBULAR-ACIDOSIS; DISEASE; FAILURE; DYSFUNCTION; SLE AB In order to estimate the frequency of functionally relevant tubular damage in SLE patients we used the presence of overt renal tubular acidosis as an indicative parameter of interstitio-tubular damage in a cohort of 109 SLE patients who were well characterized for potential Sjogren's syndrome. Only two patients had overt renal tubular acidosis and interstitial nephritis without major glomerular involvement. Both patients, aside from having systemic lupus erythematosus, had a number of clinical features of concomitant primary Sjogren's syndrome. Based on the results obtained and the analyses of previously published cases, we put forward the hypothesis of simultaneous primary Sjogren's syndrome as the prevailing associative factor for the rare occurrence of isolated interstitial nephritis in SLE. C1 UNIV VIENNA,DEPT INTERNAL MED 2,A-1010 VIENNA,AUSTRIA. NIAMSD,BETHESDA,MD. NR 41 TC 14 Z9 15 U1 0 U2 0 PU CLINICAL & EXPER RHEUMATOLOGY PI PISA PA VIA SANTA MARIA 31, 56126 PISA, ITALY SN 0392-856X J9 CLIN EXP RHEUMATOL JI Clin. Exp. Rheumatol. PD JAN-FEB PY 1991 VL 9 IS 1 BP 41 EP 45 PG 5 WC Rheumatology SC Rheumatology GA FA296 UT WOS:A1991FA29600008 PM 2054966 ER PT B AU SPORN, MB ROBERTS, AB AF SPORN, MB ROBERTS, AB BE BOCK, GR MARSH, J TI WHAT IS TGF-BETA - INTRODUCTION SO CLINICAL APPLICATIONS OF TGF-BETA SE CIBA FOUNDATION SYMPOSIA LA English DT Editorial Material CT SYMP ON CLINICAL APPLICATIONS OF TGF-BETA ( TRANSFORMING GROWTH FACTOR-BETA ) CY JUN 12-14, 1990 CL CIBA FDN, LONDON, ENGLAND SP CIBA FDN HO CIBA FDN ID TRANSFORMING GROWTH-FACTOR; PURIFICATION; CELLS; IDENTIFICATION; PROLIFERATION; SUPERFAMILY; INHIBITION; EXPRESSION; RECEPTORS; INVITRO RP SPORN, MB (reprint author), NCI,DIV CANC ETIOL,CHEMOPREVENT LAB,BLDG 41,ROOM C629,BETHESDA,MD 20892, USA. NR 0 TC 24 Z9 24 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA CHICHESTER BN 0-471-92811-9 J9 CIBA F SYMP PY 1991 VL 157 BP 1 EP 6 PG 6 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Research & Experimental Medicine; Pharmacology & Pharmacy GA BU01C UT WOS:A1991BU01C00001 PM 2070680 ER PT B AU ROBERTS, AB KIM, SJ NOMA, T GLICK, AB LAFYATIS, R LECHLEIDER, R JAKOWLEW, SB GEISER, A OREILLY, MA DANIELPOUR, D SPORN, MB AF ROBERTS, AB KIM, SJ NOMA, T GLICK, AB LAFYATIS, R LECHLEIDER, R JAKOWLEW, SB GEISER, A OREILLY, MA DANIELPOUR, D SPORN, MB BE BOCK, GR MARSH, J TI MULTIPLE FORMS OF TGF-BETA - DISTINCT PROMOTERS AND DIFFERENTIAL EXPRESSION SO CLINICAL APPLICATIONS OF TGF-BETA SE CIBA FOUNDATION SYMPOSIA LA English DT Proceedings Paper CT SYMP ON CLINICAL APPLICATIONS OF TGF-BETA ( TRANSFORMING GROWTH FACTOR-BETA ) CY JUN 12-14, 1990 CL CIBA FDN, LONDON, ENGLAND SP CIBA FDN HO CIBA FDN ID TRANSFORMING GROWTH-FACTOR; VIRUS TYPE-1 TAX; FACTOR-BETA-1 TGF-BETA-1 GENE; STIMULATED MOUSE EPIDERMIS; MESSENGER-RNA EXPRESSION; PHORBOL ESTER OCCURS; HUMAN AQUEOUS-HUMOR; T-CELL LEUKEMIA; TRANSGENIC MICE; AUTOCRINE MECHANISMS RP ROBERTS, AB (reprint author), NCI,DIV CANC ETIOL,CHEMOPREVENT LAB,BLDG 41,ROOM C629,BETHESDA,MD 20892, USA. NR 0 TC 93 Z9 93 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA CHICHESTER BN 0-471-92811-9 J9 CIBA F SYMP PY 1991 VL 157 BP 7 EP 28 PG 22 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Research & Experimental Medicine; Pharmacology & Pharmacy GA BU01C UT WOS:A1991BU01C00002 PM 1906395 ER PT J AU GREIG, NH NARIAI, T NORONHA, JG SCHMALL, B LARSON, DM SONCRANT, TT RAPOPORT, SI AF GREIG, NH NARIAI, T NORONHA, JG SCHMALL, B LARSON, DM SONCRANT, TT RAPOPORT, SI TI BRAIN-TUMOR IMAGING IN RATS USING THE POSITRON EMITTING FATTY-ACID D1-ERYTHRO-9,10-[F-18]DIFLUOROPALMITATE SO CLINICAL & EXPERIMENTAL METASTASIS LA English DT Note ID EMISSION TOMOGRAPHY AB Positron emitting dl-erythro-9,10[F-18]difluoropalmitate, [F-18]DFPA, was synthesized for the in vivo imaging of brain tumors in rats. Male Fischer 344 rats were intracerebrally implanted with Walker 256 carcinosarcoma tumor cells (1 x 10(6) in 5-mu-l tissue culture media) and 7 days later were infused with [F-18]DFPA (500-1000 mCi/mmol) i.v. for 5 min. Rats were killed after 20 min. Brains were removed and either prepared for autoradiography, or brain and tumor were separated and their radioactivity quantified by gamma spectroscopy. Brain tumors were well demarcated from surrounding and normal brain in autoradiographs, and closely paralleled tumor growth in histological sections. The mean optical density of tumor was significantly greater, by 318 +/- 68 per cent (P < 0.025, n = 3), than normal brain in autoradiographs, and that of edematous brain surrounding a large tumor was intermediately increased. [F-18]DFPA proved of value to image and circumscribe intracerebral tumors in awake rats, and studies are continuing to facilitate its clinical application in brain tumor patients. C1 NIH,DEPT NUCL MED,BETHESDA,MD 20892. RP GREIG, NH (reprint author), NIA,NEUROSCI LAB,BLDG 10,ROOM 6C103,BETHESDA,MD 20892, USA. NR 21 TC 2 Z9 2 U1 0 U2 2 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0262-0898 J9 CLIN EXP METASTAS JI Clin. Exp. Metastasis PD JAN-FEB PY 1991 VL 9 IS 1 BP 67 EP 73 DI 10.1007/BF01831711 PG 7 WC Oncology SC Oncology GA FJ834 UT WOS:A1991FJ83400007 PM 2015719 ER PT J AU ZIERDT, CH AF ZIERDT, CH TI BLASTOCYSTIS-HOMINIS - PAST AND FUTURE SO CLINICAL MICROBIOLOGY REVIEWS LA English DT Article AB Blastocystis hominis, classified as a yeast in 1912, was reclassified in 1967 as a protozoan and assigned to the family Sarcodina (amebae) in 1988. Protean morphology characterizes this human intestinal parasite and has delayed its recognition as a pathogen. An anaerobe, B. hominis has no cyst form. Typically, in clinical specimens, the entire center of the spherical cells is taken up with a membrane-bounded sphere, the central body, involved with asexual reproduction. In some infections, an ameba form may predominate or a third form, the granule cell, named for the highly visible mitochondria occupying the cytoplasm. The function of these mitochondria in the anaerobic B. hominis cell is not yet known but may be related to lipid synthesis. Diagnosis is made by microscopic examination of fresh or preserved concentrated fecal specimens, as either wet mounts or permanently stained smears. Reports of B. hominis in patients with gastrointestinal disease continue to increase, most of which support the concept that this protozoan is pathogenic. symptomatic infection often resolves within 3 days and is not treated. Chronic symptomatic cases are frequent, however, and may be incapacitating and require therapy. In recent years treatment has been almost exclusively with metronidazole. RP ZIERDT, CH (reprint author), NIH,DEPT CLIN PATHOL,MICROBIOL SERV,BETHESDA,MD 20892, USA. NR 0 TC 178 Z9 196 U1 0 U2 10 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0893-8512 J9 CLIN MICROBIOL REV JI Clin. Microbiol. Rev. PD JAN PY 1991 VL 4 IS 1 BP 61 EP 79 PG 19 WC Microbiology SC Microbiology GA ER202 UT WOS:A1991ER20200006 PM 2004348 ER EF