FN Thomson Reuters Web of Science™ VR 1.0 PT J AU TOCHNER, Z MITCHELL, JB HOEKSTRA, HJ SMITH, P DELUCA, AM BARNES, M HARRINGTON, F MANYAK, M RUSSO, D RUSSO, A AF TOCHNER, Z MITCHELL, JB HOEKSTRA, HJ SMITH, P DELUCA, AM BARNES, M HARRINGTON, F MANYAK, M RUSSO, D RUSSO, A TI PHOTODYNAMIC THERAPY OF THE CANINE PERITONEUM - NORMAL TISSUE-RESPONSE TO INTRAPERITONEAL AND INTRAVENOUS PHOTOFRIN FOLLOWED BY 630NM LIGHT SO LASERS IN SURGERY AND MEDICINE LA English DT Article DE ABDOMINAL CARCINOMATOSIS; PERITONEAL CARCINOMATOSIS; INTRAPERITONEAL PHOTODYNAMIC THERAPY; PHOTODYNAMIC THERAPY ID CHEMOTHERAPY AB A toxicity study was performed in a canine model to explore the feasibility of using intraperitoneal photodynamic therapy for patients with peritoneal carcinomatosis. Dogs received 1.25 mg/kg Photofrin II both intravenously (48 hours) and intraperitoneally (2 hours) before intraperitoneal light treatment. The entire peritoneal surface was illuminated with 0.57-0.74 J/cm2 of red light (630 nm). Thirteen dogs were treated: 4 control and 9 full treatment. Of the fully treated dogs, 5 received a single treatment and 4 received 3 treatments. The dogs were evaluated clinically and pathologically for immediate (4 days) and late (60 days) side effects. All animals tolerated the treatment without significant morbidity. Postoperative blood tests were normal except for low lymphocyte counts and elevated liver transaminases, both of which normalized rapidly. Histopathologically, there was approximately a 50% incidence of mild inflammatory peritoneal response. This study provides the basis for a Phase I human trial. C1 NCI,DIV BIOENGN,BETHESDA,MD 20892. UNIV HOSP GRONINGEN,DIV SURG ONCOL,GRONINGEN,NETHERLANDS. RP TOCHNER, Z (reprint author), NCI,RADIAT ONCOL BRANCH,BLDG 10,ROOM B3B69,BETHESDA,MD 20892, USA. NR 12 TC 15 Z9 16 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0196-8092 J9 LASER SURG MED JI Lasers Surg. Med. PY 1991 VL 11 IS 2 BP 158 EP 164 DI 10.1002/lsm.1900110210 PG 7 WC Dermatology; Surgery SC Dermatology; Surgery GA FE653 UT WOS:A1991FE65300009 PM 1827854 ER PT J AU CHESON, BD VENA, DA SORENSEN, JM FALLAVOLLITA, A AF CHESON, BD VENA, DA SORENSEN, JM FALLAVOLLITA, A TI CURRENT STATUS OF UNITED-STATES CLINICAL-TRIALS IN CHRONIC LYMPHOCYTIC-LEUKEMIA SO LEUKEMIA & LYMPHOMA LA English DT Article; Proceedings Paper CT 5TH INTERNATIONAL WORKSHOP ON CLL ( CHRONIC-LYMPHOCYTIC-LEUKEMIA ) CY APR 26-28, 1991 CL BARCELONA, SPAIN SP BERLEX LABS, BRISTOL MYERS SQUIBB, SPANISH ONCOL LINE, CILAG, MENARINI DIAGNOSTICOS, SCHERING PLOUGH INT, FONDO INVESTIGAC SANITARIAS SEGURIDAD SOCIAL, UNIV BARCELONA, DIV CIEN SALUT DE CLL; LEUKEMIA; CLINICAL TRIALS RP CHESON, BD (reprint author), NCI,DIV CANC TREATMENT,CANC THERAPY EVALUAT PROGRAM,EXECUT PLAZA N,ROOM 741,BETHESDA,MD 20892, USA. NR 0 TC 13 Z9 13 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1042-8194 J9 LEUKEMIA LYMPHOMA JI Leuk. Lymphoma PY 1991 VL 5 SU S BP 119 EP 122 DI 10.3109/10428199109103391 PG 4 WC Oncology; Hematology SC Oncology; Hematology GA GZ115 UT WOS:A1991GZ11500021 PM 27463492 ER PT J AU CAPORASO, NE WHITEHOUSE, J BERTIN, P AMOS, C PAPADOPOULOS, N MULLER, J WHANGPENG, J TUCKER, MA FLEISHER, TA MARTI, GE AF CAPORASO, NE WHITEHOUSE, J BERTIN, P AMOS, C PAPADOPOULOS, N MULLER, J WHANGPENG, J TUCKER, MA FLEISHER, TA MARTI, GE TI A 20 YEAR CLINICAL AND LABORATORY STUDY OF FAMILIAL B-CHRONIC LYMPHOCYTIC-LEUKEMIA IN A SINGLE KINDRED SO LEUKEMIA & LYMPHOMA LA English DT Article DE FAMILY STUDIES; B-CLL; LYMPHOCYTIC LEUKEMIA; SINGLE KINDRED; CLL RP CAPORASO, NE (reprint author), NCI,EPIDEMIOL & BIOSTAT PROGRAM,FAMILY STUDIES SECT,439E EXECUT PLAZA N,BETHESDA,MD 20892, USA. RI Tucker, Margaret/B-4297-2015 NR 0 TC 9 Z9 9 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1042-8194 J9 LEUKEMIA LYMPHOMA JI Leuk. Lymphoma PY 1991 VL 3 IS 5-6 BP 331 EP & DI 10.3109/10428199109070277 PG 0 WC Oncology; Hematology SC Oncology; Hematology GA EY706 UT WOS:A1991EY70600004 PM 27467424 ER PT J AU GREM, JL PLOWMAN, J RUBINSTEIN, L HAWKINS, MJ HARRISON, SD AF GREM, JL PLOWMAN, J RUBINSTEIN, L HAWKINS, MJ HARRISON, SD TI MODULATION OF CYTOSINE-ARABINOSIDE TOXICITY BY 3-DEAZAURIDINE IN A MURINE LEUKEMIA MODEL SO LEUKEMIA RESEARCH LA English DT Article DE ARA-C; 3-DEAZAURIDINE; LEUKEMIA ID 1-BETA-D-ARABINOFURANOSYLCYTOSINE; ANTITUMOR; RESISTANT AB 3-Deazauridine (DAUrd), a competitive inhibitor of CTP synthetase, inhibits both RNA and DNA synthesis. Murine leukemia cells resistant to cytosine arabinoside (ara-C) due to a deletion of deoxycytidine kinase are collaterally sensitive to DAUrd, which inhibits the de novo production of CTP and hence results in dCTP depletion. We evaluated DAUrd in combination with the palmitate derivative of ara-C (palmO-ara-C) in mice bearing L1210 leukemia cells with a subpopulation resistant to ara-C. Both simultaneous administration and a sequential schedule of palmO-ara-C at its maximally tolerated dose (MTD), followed by DAUrd treatment, failed to produce a therapeutic gain. We also studied whether non-toxic doses of DAUrd (15-250 mg/kg i.p. at h 0 and 6 on days 4 and 8) could modulate the antileukemic activity of palmO-ara-C (7.5-120 mg/kg i.p. at h 3 on days 4 and 8). The addition of DAUrd produced a modest (but statistically significant) prolongation of life span and a further 2-log10 reduction in tumor burden compared to the same dose of palmO-ara-C alone, and resulted in long-term survivors in five of 30 treated animals. Two-dimensional dose-response analysis of the survival data indicated a positive drug interaction (p less-than-or-equal-to 0.01) when the dosage of DAUrd was modeled to reflect an apparent threshold efect. Cyclopentenyl cytosine (CPE-C; 0.625-2.5 mg/kg i.p. at h 0 and 6 on days 4 and 8), a more potent inhibitor of CTP synthetase, was also given with palmO-ara-C. This combination resulted in an additional 2-6 log10 units of cell kill and occasional long-term survivors at palmO-ara-C dosages that alone resulted in no more than 2 log10 units of cell kill and no long-term survivors. However, DAUrd and CPE-C given with palmO-ara-C increased host toxicity, compromising the tolerable dose of palmO-ara-C. Single-agent palmO-ara-C given at its MTD produced a similar reduction in tumor burden and increase in life span compared to the highest palmO-ara-C dose that could be given in combination with either modulator. C1 NCI,PHARMACOL BRANCH,DEV THERAPEUT PROGRAM,BETHESDA,MD 20892. NCI,BIOMETR RES BRANCH,BETHESDA,MD 20892. NCI,DIV CANC TREATMENT,CANC THERAPY EVALUAT PROGRAM,INVEST DRUG BRANCH,BETHESDA,MD 20892. SO RES INST,BIRMINGHAM,AL 35255. RP GREM, JL (reprint author), NCI,MED BRANCH,CLIN ONCOL PROGRAM,DCT,BLDG 10,ROOM 12N226,BETHESDA,MD 20892, USA. FU NCI NIH HHS [N01-CM-67724] NR 20 TC 10 Z9 10 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0145-2126 J9 LEUKEMIA RES JI Leuk. Res. PY 1991 VL 15 IS 4 BP 229 EP 236 DI 10.1016/0145-2126(91)90125-D PG 8 WC Oncology; Hematology SC Oncology; Hematology GA FJ132 UT WOS:A1991FJ13200009 PM 2030604 ER PT J AU POTTERN, LM LINET, M BLAIR, A DICK, F BURMEISTER, LF GIBSON, R SCHUMAN, LM FRAUMENI, JF AF POTTERN, LM LINET, M BLAIR, A DICK, F BURMEISTER, LF GIBSON, R SCHUMAN, LM FRAUMENI, JF TI FAMILIAL CANCERS ASSOCIATED WITH SUBTYPES OF LEUKEMIA AND NON-HODGKINS-LYMPHOMA SO LEUKEMIA RESEARCH LA English DT Article DE LEUKEMIA; LYMPHOMA; FAMILIAL CANCERS; CASE-CONTROL ID CHRONIC LYMPHOCYTIC-LEUKEMIA; YORKSHIRE; DISEASE; MALIGNANCIES; RELATIVES; HISTORY AB To investigate whether a history of hematolymphoproliferative cancers (HLP) and other cancers among a parent or sibling is a risk factor for specific subtypes of leukemia and non-Hodgkin's lymphoma (NHL), data from a population-based case-control study, in Iowa and Minnesota, of 578 leukemia cases, 622 NHL cases and 1245 controls were evaluated. Having at least one sibling with HLP significantly increased the risk for all leukemias combined (odds ratio (OR) = 2.3) and for NHL (OR = 2.7). In particular, chronic lymphocytic leukemia (CLL) was significantly increased among those reporting a sibling with leukemia (OR = 3.0) or lymphoma (OR = 4.3). Elevated risksof small lymphocytic NHL(SML)(OR = 7.3) and diffuse NHL (DIF)(OR =5.4) were also observed among subjects who had a sibling with lymphoma (primarily Hodgkin's disease). A significantly increased risk of follicular NHL was noted among those with a sibling history of pancreatic cancer (OR = 4.8)and colorectal cancer (OR = 2.7). Parental history of HLP was not associated with any type of leukemia or NHL. A history of stomach cancer among parents was associated with a 2-fold elevation of CLL and DIF compared to controls. Increased risks of CLL and DIF were also linked to breast cancer among sisters and mothers, respectively. Prostate cancer among fathers increased the risk 2-fold for CLL and 3-fold for SML. This study confirms some familial cancer associations previously reported for leukemia and NHL, and provides new information regarding the various subtypes of leukemia and NHL. C1 UNIV IOWA,DEPT PREVENT MED,IOWA CITY,IA 52242. UNIV MINNESOTA,DEPT EPIDEMIOL,MINNEAPOLIS,MN 55455. RP POTTERN, LM (reprint author), NCI,DIV CANC ETIOL,EPIDEMIOL & BIOSTAT PROGRAM,EXECUT PLAZA N,ROOM 418,BETHESDA,MD 20892, USA. NR 36 TC 91 Z9 93 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0145-2126 J9 LEUKEMIA RES JI Leuk. Res. PY 1991 VL 15 IS 5 BP 305 EP 314 DI 10.1016/0145-2126(91)90005-E PG 10 WC Oncology; Hematology SC Oncology; Hematology GA FN914 UT WOS:A1991FN91400005 PM 2046383 ER PT J AU RICHARDS, DT AF RICHARDS, DT TI BY YOUR SELECTION CRITERIA ARE YE KNOWN SO LIBRARY ACQUISITIONS-PRACTICE AND THEORY LA English DT Article RP RICHARDS, DT (reprint author), NATL LIB MED,BETHESDA,MD 20894, USA. NR 12 TC 4 Z9 4 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0364-6408 J9 LIBR ACQUIS PRACT TH JI Libr. Acquis.-Pract. Theory PY 1991 VL 15 IS 3 BP 279 EP 285 DI 10.1016/0364-6408(91)90027-C PG 7 WC Information Science & Library Science SC Information Science & Library Science GA GE775 UT WOS:A1991GE77500005 ER PT J AU GILLETTE, JR AF GILLETTE, JR TI INSIGHTS INTO DEUTERIUM-ISOTOPE EFFECTS ON THE METABOLISM OF STEROID-HORMONES AND FOREIGN COMPOUNDS BY CYTOCHROME-P-450 ENZYMES SO LIFE SCIENCES LA English DT Article ID RATE-LIMITING STEP; TESTOSTERONE; MECHANISM RP GILLETTE, JR (reprint author), NHLBI,CHEM PHARMACOL LAB,BETHESDA,MD 20892, USA. NR 9 TC 6 Z9 6 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 49 IS 1 BP 1 EP 14 DI 10.1016/0024-3205(91)90573-T PG 14 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA FN907 UT WOS:A1991FN90700001 PM 2051881 ER PT J AU CARR, DJJ DECOSTA, BR JACOBSON, AE RICE, KC BLALOCK, JE AF CARR, DJJ DECOSTA, BR JACOBSON, AE RICE, KC BLALOCK, JE TI ENANTIOSELECTIVE KAPPA OPIOID BINDING-SITES ON THE MACROPHAGE CELL-LINE, P388D SO LIFE SCIENCES LA English DT Article ID IMMUNE-SYSTEM; RECEPTOR SUBTYPES; DELTA-CLASS; RAT-BRAIN; ANTAGONISTS; PEPTIDES; AGONISTS; U50,488 AB A kappa (kappa) opioid binding site has been characterized on the macrophage cell line, P388d1, using the kappa selective affinity ligand, [H-3](1S,2S)-(-)-trans-2-isothiocyanato-N-methyl-N-[2-(1-pyrrolidinyl) cyclohexyl] benzeneacetamide (-)BD166). The kappa site has a relative molecular mass (Mr) of 38,000 under nonreducing conditions and 42,000 under reducing conditions. Moreover, it exhibits enantioselectivity in that 1S,2S-(-)-trans-3,4-dichloro-N-methyl-N-[2-1-pyrrolidinyl)cyclohexyl] benzeneacetamide ((-)U-50,488) blocks [H-3](5-alpha,7-alpha,8-beta)-(-)-N-methyl-N-[7-(1- pyrrolidinyl)-1-oxaspiro-(4,5)-dec-8-yl]benzeneacetamide (U-69,593) binding to P388d1 cells with an IC50 = 7.0 nM whereas 1R,2R-(+)-trans-3,4-dichloro-N-methyl-N-[2-(1-pyrrolidinyl)cyclohexyl] benzeneacetamide ((+)U-50,488) blocks [H-3]U-69,593 binding to P388d1 cells with an IC50 = 700 nM. C1 NIDDK,MED CHEM LAB,BETHESDA,MD 20892. RP CARR, DJJ (reprint author), UNIV ALABAMA,DEPT PHYSIOL & BIOPHYS,UAB STN BHSB 890,BIRMINGHAM,AL 35294, USA. NR 18 TC 24 Z9 24 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 49 IS 1 BP 45 EP 51 DI 10.1016/0024-3205(91)90578-Y PG 7 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA FN907 UT WOS:A1991FN90700006 PM 1646923 ER PT J AU WOZNIAK, KM LINNOILA, M AF WOZNIAK, KM LINNOILA, M TI HYPERGLYCEMIC PROPERTIES OF SEROTONIN RECEPTOR ANTAGONISTS SO LIFE SCIENCES LA English DT Article ID INSULIN-SECRETION; PHYSIOLOGICAL FUNCTIONS; PANCREATIC-ISLETS; 5-HT1A RECEPTORS; PLASMA-GLUCOSE; CONSCIOUS RAT; 8-OH-DPAT; MICE; 5-HYDROXYTRYPTAMINE; DRUGS AB Several serotonin (5-HT) receptor antagonists with varying specificities for the 5-HT receptor types, were studied with regard to their effects on blood glucose levels in mice. The non-selective antagonists, metergoline and methysergide, proved to be hyperglycemic at doses commonly used to antagonize 5-HT receptors. In contrast, ritanserin (a 5-HT2 and 5-HT1C antagonist) and MDL 72222 (a 5-HT3 antagonist) were effective only at doses which surpassed the dose range considered to be selective for their respective receptors. The results suggest that 5-HT systems play a role in maintaining glucose homeostasis and that 5-HT1 receptors may be particularly important in this function. Furthermore, the inherent hyperglycemic properties of non-selective serotonin antagonists described here, are pertinent to studies using these agents to investigate glucose metabolism. RP WOZNIAK, KM (reprint author), NIAAA,CLIN STUDIES LAB,DICBR,BLDG 10 3C102,BETHESDA,MD 20892, USA. NR 47 TC 36 Z9 43 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 49 IS 2 BP 101 EP 109 DI 10.1016/0024-3205(91)90023-5 PG 9 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA FQ198 UT WOS:A1991FQ19800003 PM 1905770 ER PT J AU PICKWORTH, WB BUNKER, E WELCH, P CONE, E AF PICKWORTH, WB BUNKER, E WELCH, P CONE, E TI INTRAVENOUS BUPRENORPHINE REDUCES PUPIL SIZE AND THE LIGHT REFLEX IN HUMANS SO LIFE SCIENCES LA English DT Article ID MORPHINE-INDUCED MYDRIASIS; EDINGER-WESTPHAL NUCLEUS; ADDICTION; CHALLENGE; AGONIST; OPIOIDS; AGENT; DOG; RAT AB The pupillary effects of intravenous buprenorphine were studied in eight nondependent male subjects who reported previous opiate use. Buprenorphine (0.3, 0.6, and 1.2 mg) decreased pupil size, the amplitude of the light reflex, and the velocities of constriction and dilation. Significant pupillary effects occurred within 15 min of the injection and persisted for 24 hr. At 48 hr most measures returned to baseline levels. Generally the magnitude of the effect was not dose related although recovery occurred sooner after the lower dose. The time course of the pupillary effects of buprenorphine exceeds duration of its analgesic and subjective effects. Previous studies have reported that pupillary measures are especially sensitive to the acute effects of full opiate agonists. The results of the present study indicate that buprenorphine, a partial opiate agonist, causes profound and persistent effects on pupillary size and dynamic measures. RP PICKWORTH, WB (reprint author), NIDA,ADDICT RES CTR,BALTIMORE,MD 21224, USA. NR 30 TC 11 Z9 11 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 49 IS 2 BP 129 EP 138 DI 10.1016/0024-3205(91)90026-8 PG 10 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA FQ198 UT WOS:A1991FQ19800006 PM 2062170 ER PT J AU SEI, Y MCINTYRE, T SKOLNICK, P ARORA, PK AF SEI, Y MCINTYRE, T SKOLNICK, P ARORA, PK TI STRESS MODULATES CALCIUM MOBILIZATION IN IMMUNE CELLS SO LIFE SCIENCES LA English DT Article ID MICE; LYMPHOCYTES; BEREAVEMENT; STIMULATION; TURNOVER; CA-2+ AB Both acute and chronic restraint stress modulated mitogen-induced increases in cytoplasmic free-calcium concentrations ([Ca2+]i) in mouse spleen cells. Dual-color analysis of lymphocyte subpopulations demonstrated that acute (2 hour) restraint stress suppressed mitogen-stimulated increases in [Ca2+]i in CD4+ T cells, but enhanced [Ca2+]i in CD8+ T cells. Chronic restraint stress (2 hours daily for up to 21 days) resulted in a significant suppression of mitogen-stimulated increases in [Ca2+]i in CD4+ T cells at 3 and 7 days, but not at 21 days. CD8+T cells were unaffected by chronic stress. Chronic stress (for 7 days) had a modest suppressive effect on mitogen-induced Ca2+ responses in B cells. Within T lymphocyte subpopulations, both acute and chronic stress predominantly affected CD4+ T cells, which may induce a functional reversal of the CD4/CD8 ratios in vivo. Such a reversal could result in suppression of a variety of immune responses such as lymphocyte proliferation and antigen-specific antibody production. These findings indicate that the inhibitory effects of stress on calcium mobilization in lymphocytes may be an early event mediating stress-induced immunosuppression. RP SEI, Y (reprint author), NIDDKD,NEUROSCI LAB,BETHESDA,MD 20892, USA. NR 28 TC 9 Z9 9 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 49 IS 9 BP 671 EP 676 DI 10.1016/0024-3205(91)90114-Q PG 6 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA FY540 UT WOS:A1991FY54000005 PM 1865761 ER PT J AU UEEDA, M THOMPSON, RD PADGETT, WL SECUNDA, S DALY, JW OLSSON, RA AF UEEDA, M THOMPSON, RD PADGETT, WL SECUNDA, S DALY, JW OLSSON, RA TI CARDIOVASCULAR ACTIONS OF ADENOSINES, BUT NOT ADENOSINE RECEPTORS, DIFFER IN RAT AND GUINEA-PIG SO LIFE SCIENCES LA English DT Article ID SPECIES-DIFFERENCES; STRIATAL MEMBRANES; CONDUCTION; MECHANISM; BRAIN AB This study compared the structure-activity relationships of 16 analogues at the A1 and A2 adenosine receptors (A1AR, A2AR) of rat and guinea pig. Radioligand binding studies revealed no marked differences in the affinities of each analogue at the A1AR of brain cortex or the A2AR of brain striatum. Bioassay employing Langendorff heart preparations showed that the guinea pig is more sensitive than the rat to A1AR-mediated slowing of conduction through the atrioventricular node and, in some instances, to A2AR-mediated coronary vasodilation. That difference could reflect factors such as receptor density or efficacy of coupling to effector systems. C1 UNIV S FLORIDA,BIOCHEM & MOLEC BIOL,TAMPA,FL 33612. NATL INST DIABET & DIGEST & KIDNEY,BIOORGAN CHEM LAB,BETHESDA,MD 20892. RP UEEDA, M (reprint author), UNIV S FLORIDA,DEPT INTERNAL MED,TAMPA,FL 33612, USA. NR 13 TC 25 Z9 25 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 49 IS 18 BP 1351 EP 1358 DI 10.1016/0024-3205(91)90199-L PG 8 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA GG817 UT WOS:A1991GG81700010 PM 1921650 ER PT J AU FUDALA, PJ BERKOW, LC FRALICH, JL JOHNSON, RE AF FUDALA, PJ BERKOW, LC FRALICH, JL JOHNSON, RE TI USE OF NALOXONE IN THE ASSESSMENT OF OPIATE DEPENDENCE SO LIFE SCIENCES LA English DT Article ID PHYSICAL-DEPENDENCE AB All subjects participating in an outpatient study comparing treatments for opiate dependence were given a naloxone challenge to document their level of dependence. Subjects were assessed at 0, 10, 20, and 30 minutes following the administration of intramuscular naloxone (0.4 mg) using an opiate withdrawal assessment scale and measurements of pupillary diameter. Subjects' self reports of daily dollar amounts of opiate use and time since last use were also examined for possible correlation with withdrawal scale scores and pupillary measurements. A significant negative correlation was obtained between pupil diameter and time since last reported use of an opiate. Results indicated that the scale was a reliable indicator of opiate dependence. Ways in which it might be improved are discussed. RP FUDALA, PJ (reprint author), NIDA,ADDICT RES CTR,BALTIMORE,MD 21224, USA. NR 13 TC 5 Z9 5 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 49 IS 24 BP 1809 EP 1814 DI 10.1016/0024-3205(91)90482-Q PG 6 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA GN754 UT WOS:A1991GN75400006 PM 1943484 ER PT J AU HENG, X QING, N JACOBSON, AE RICE, KC ROTHMAN, RB AF HENG, X QING, N JACOBSON, AE RICE, KC ROTHMAN, RB TI PRELIMINARY LIGAND-BINDING DATA FOR SUBTYPES OF THE DELTA OPIOID RECEPTOR IN RAT-BRAIN MEMBRANES SO LIFE SCIENCES LA English DT Letter ID OPIATE RECEPTORS; INVITRO; INVIVO; SITE AB Delta opioid binding sites were assayed using [H-3][D-ala2,D-leu5]enkephalin and rat brain membranes depleted of mu-binding sites with the site-directed acylating agent, 2-(pethoxybenzyl)-1-diethylaminoethyl-5-isothiocyanatobenzimidazole-HCI. [D-Pen2,D-Pen5]enkephalin (DPDPE), [D-Pen2,L-Pen5]enkephalin, [D-Ala2]deltorphin-I and [D-Ala2]deltorphin-II inhibition curves were characterized by slope factors (Hill coefficients) less than 1. The low slope factor of DPDPE persisted in the presence of 50-mu-M 5'-guanylyimidodiphosphate in the assay Quantitative analysis of [D-ala2,D-leu5]enkephalin, DPDPE and [D-Ala2]deltorphin-I binding surfaces resolved two binding sites. Whereas [D-ala2,D-leu5]enkephalin had equal affinity for both sites, DPDPE and [D-Ala2]deltorphin-I had high affinity for the high capacity binding site, and low affinity for the low capacity binding site. These data support pharmacological studies demonstrating delta-receptor subtypes which mediate antinociception. C1 NATL INST DRUG ABUSE,ADDICT RES CTR,CLIN PSYCHOPHARMACOL LAB,POB 5180,BALTIMORE,MD 21224. NATL INST DIABET & DIGEST & KIDNEY,MED CHEM LAB,BETHESDA,MD 20892. NR 18 TC 0 Z9 0 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 49 IS 18 BP PL141 EP PL146 PG 6 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA GG817 UT WOS:A1991GG81700015 ER PT J AU FACCHIANO, A CORDELLAMIELE, E MIELE, L MUKHERJEE, AB AF FACCHIANO, A CORDELLAMIELE, E MIELE, L MUKHERJEE, AB TI INHIBITION OF PANCREATIC PHOSPHOLIPASE-A2 ACTIVITY BY UTEROGLOBIN AND ANTIFLAMMIN PEPTIDES - POSSIBLE MECHANISM OF ACTION SO LIFE SCIENCES LA English DT Article ID AGKISTRODON-PISCIVORUS-PISCIVORUS; PLATELET-AGGREGATION; SEQUENCE HOMOLOGY; LIPOCORTIN-I; REGION; ENZYME; ACTIVATION; PROTEINS; BIOLOGY; GENE AB We investigated the possible mechanism of inhibition of porcine pancreatic phospholipase A2 in vitro by rabbit uteroglobin and by the antiflammin peptides. We optimized the conditions of phospholipase A2 assay using a deoxycholate-phosphatidylcholine mixed micellar substrate and established the activity of these inhibitors under optimized conditions. The results of fluorescence studies and crosslinking experiments indicate that the inhibitors interact with the enzyme in solution and affect the increase in intrinsic fluorescence of phospholipase A2 observed upon interaction with a mixed micellar substrate. In addition, we identified a sequence similarity between the antiflammin peptides, the putative active region of uteroglobin and a region in pancreatic phospholipase A2. This region of phospholipase A2 has been previously identified as being involved in the regulation of dimerization of this enzyme, and is conserved in the pancreatic-type enzymes. Taken together, these observations suggest that uteroglobin and antiflammins interact with porcine pancreatic phospholipase A2 and this may, at least in part, explain the enzyme inhibitory effect of these molecules observed in vitro. One possible mechanism of this effect may be an interference with the dimerization process of phospholipase A2 which is associated with interfacial activation. C1 NICHHD,HUMAN GENET BRANCH,DEV GENET SECT,BLDG 10,RM 95242,BETHESDA,MD 20892. RI Facchiano, Antonio/K-5984-2016 OI Facchiano, Antonio/0000-0002-4243-2392 NR 36 TC 52 Z9 52 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 48 IS 5 BP 453 EP 464 DI 10.1016/0024-3205(91)90501-2 PG 12 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA EQ046 UT WOS:A1991EQ04600010 PM 1992287 ER PT J AU CHOCHUNG, YS CLAIR, T TORTORA, G YOKOZAKI, H PEPE, S AF CHOCHUNG, YS CLAIR, T TORTORA, G YOKOZAKI, H PEPE, S TI SUPPRESSION OF MALIGNANCY TARGETING THE INTRACELLULAR SIGNAL TRANSDUCING PROTEINS OF CAMP - THE USE OF SITE-SELECTIVE CAMP ANALOGS, ANTISENSE STRATEGY, AND GENE-TRANSFER SO LIFE SCIENCES LA English DT Review ID CYCLIC-AMP ANALOGS; ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE; II REGULATORY SUBUNIT; CANCER CELL-GROWTH; BINDING-SITES; CATALYTIC SUBUNIT; KINASE ISOZYMES; MOLECULAR-CLONING; RAT FIBROBLASTS; DIFFERENTIATION AB An hypothesis has been presented suggesting that two isoforms of cAMP receptor proteins are crucial effectors in tumorigenesis. The evidence in support of this hypothesis shows that: (1) cAMP transduces dual controls, both positive and negative, on cell growth and differentiation. (2) Such dual controls are respectively governed by two isoforms of cAMP receptor proteins, the type I and type II regulatory subunits of cAMP-dependent protein kinase. (3) In normal physiology, the functional balance of these cAMP receptor isoforms is strictly controlled to meet either stimulation or inhibition of cell growth as it is required, whereas such control is lost in cancer cells. (4) Cancer cells can also be made to differentiate and acquire growth control when the functional balance of these intracellular signal transducers of cAMP is restored by the use of site-selective cAMP analogs, antisense strategy, or gene transfer, suggesting new approaches to cancer therapy. RP CHOCHUNG, YS (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,CELLULAR BIOCHEM SECT,BLDG 10,ROOM 5B38,BETHESDA,MD 20892, USA. OI Yokozaki, Hiroshi/0000-0001-5276-3331 NR 74 TC 35 Z9 36 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 48 IS 12 BP 1123 EP 1132 DI 10.1016/0024-3205(91)90449-L PG 10 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA EY111 UT WOS:A1991EY11100001 PM 1848339 ER PT J AU KONICKI, PE OWEN, RR LITMAN, RE PICKAR, D AF KONICKI, PE OWEN, RR LITMAN, RE PICKAR, D TI THE ACUTE EFFECTS OF CENTRAL-ACTING AND PERIPHERAL-ACTING DOPAMINE ANTAGONISTS ON PLASMA HVA IN SCHIZOPHRENIC-PATIENTS SO LIFE SCIENCES LA English DT Article ID HOMOVANILLIC-ACID CONCENTRATION; HALOPERIDOL TREATMENT; INDUCED INCREASE; CELL ACTIVITY; METABOLISM; BRAIN; NEUROLEPTICS; DEBRISOQUIN; FLUPHENAZINE; DOMPERIDONE AB The short-term effects of fluphenazine on plasma HVA concentrations were compared with the effects of fluphenazine and concurrent administration of debrisoquin, a monoamine oxidase inhibitor which does not cross the blood brain barrier and is used to enhance the CNS contribution to circulating plasma HVA concentrations. Fluphenazine significantly increased plasma HVA with or without debrisoquin 24 hours following the initiation of treatment. Domperidone, a butyrophenone dopamine antagonist which acts only in the peripheral nervous system, failed to alter plasma HVA concentrations. These data suggest that the acute effects of neuroleptic drugs on plasma HVA concentrations are dependent upon interaction with CNS dopaminergic systems and provide additional support for the use of plasma HVA as a reflection of CNS dopamine system activity in clinical studies. RP KONICKI, PE (reprint author), NIMH,CLIN NEUROSCI BRANCH,BLDG 10,RM 4N214,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 25 TC 18 Z9 18 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 48 IS 14 BP 1411 EP 1416 PG 6 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA EZ906 UT WOS:A1991EZ90600009 PM 2008157 ER PT J AU SCHINDLER, CW TELLA, SR WITKIN, JM GOLDBERG, SR AF SCHINDLER, CW TELLA, SR WITKIN, JM GOLDBERG, SR TI EFFECTS OF COCAINE ALONE AND IN COMBINATION WITH HALOPERIDOL AND SCH 23390 ON CARDIOVASCULAR FUNCTION IN SQUIRREL-MONKEYS SO LIFE SCIENCES LA English DT Article ID DOPAMINE-D2 RECEPTOR AGONIST; INTRAVENOUS COCAINE; NONHUMAN-PRIMATES; RAT; LY171555; RELEASE; BRAIN; DRUGS AB The potential involvement of D1 and D2 dopamine receptors in the effects of cocaine on cardiovascular function in squirrel monkeys was evaluated. A low dose of cocaine (0.1 mg/kg i.v.) produced increases in both blood pressure and heart rate. At the higher doses of cocaine (1.0-3.0 mg/kg) the heart rate response was biphasic, consisting of an early decrease followed by an increase in heart rate 10-20 min following injection. The dopamine D2 antagonist haloperidol (0.01 mg/kg i.m.) attenuated the heart rate increasing effect of cocaine, but doses as high as 0.03 mg/kg did not alter the blood pressure increase. The D1 antagonist SCH 23390 (0.01-0.03 mg/kg i.m.) did not attenuate either the blood pressure or heart rate increasing effects of cocaine. The D2 agonist quinpirole (1.0 mg/kg i.v.) produced increases in heart rate similar to cocaine, with little effect on blood pressure. Although effective against the heart rate increasing effect of cocaine, haloperidol (0.01 mg/kg) did not antagonize the heart rate increasing effects of quinpirole. The D1 agonist SKF 38393 (3.0 mg/kg i.v.) decreased heart rate and increased blood pressure. The blood pressure increasing effect of SKF 38393 was antagonized by 0.01 mg/kg SCH 23390. Haloperidol's ability to partially antagonize the tachycardiac response to cocaine suggests the involvement of D2 receptors in that response. However, the failure of haloperidol to antagonize quinpirole's tachycardiac effect suggests that non-dopaminergic mechanisms may also be involved in haloperidol's antagonism of cocaine's tachycardiac effect. The pressor effects of cocaine do not appear to be controlled by selective dopamine receptors. C1 UNIV MARYLAND,DEPT PHARMACOL & EXPTL THERAPEUT,BALTIMORE,MD 21201. GEORGETOWN UNIV,SCH MED,DEPT PHARMACOL,WASHINGTON,DC 20007. RP SCHINDLER, CW (reprint author), NIDA,ADDICT RES CTR,PRECLIN PHARMACOL BRANCH,POB 5180,BALTIMORE,MD 21224, USA. NR 28 TC 25 Z9 25 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 48 IS 16 BP 1547 EP 1554 DI 10.1016/0024-3205(91)90279-K PG 8 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA FB842 UT WOS:A1991FB84200006 PM 2016988 ER PT J AU SZALLASI, A BLUMBERG, PM AF SZALLASI, A BLUMBERG, PM TI MOLECULAR TARGET SIZE OF THE VANILLOID (CAPSAICIN) RECEPTOR IN PIG DORSAL-ROOT GANGLIA SO LIFE SCIENCES LA English DT Article ID RADIATION INACTIVATION; RAT-BRAIN; COMPLEX AB The size of the vanilloid receptor was examined by high-energy radiation inactivation analysis of the binding of [H-3]resiniferatoxin to pig dorsal root ganglion membranes; it was found to be 270 +/- 25 kDa. This value most likely represents the size of a receptor complex rather than of an individual subunit. Other ligand-gated cation channel complexes have reported molecular weights in this range, e.g. 300 kDa for the acetylcholine receptor. RP SZALLASI, A (reprint author), NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892, USA. NR 22 TC 33 Z9 33 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 48 IS 19 BP 1863 EP 1869 DI 10.1016/0024-3205(91)90242-4 PG 7 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA FF076 UT WOS:A1991FF07600009 PM 1645836 ER PT J AU IADAROLA, MJ OFRI, D KLEINMAN, JE AF IADAROLA, MJ OFRI, D KLEINMAN, JE TI ENKEPHALIN, DYNORPHIN AND SUBSTANCE-P IN POSTMORTEM SUBSTANTIA-NIGRA FROM NORMALS AND SCHIZOPHRENIC-PATIENTS SO LIFE SCIENCES LA English DT Article ID ALPHA-NEO-ENDORPHIN; VENTRAL TEGMENTAL AREA; CENTRAL NERVOUS-SYSTEM; RAT-BRAIN; BASAL GANGLIA; HUNTINGTONS-DISEASE; LEU-ENKEPHALIN; METHIONINE-ENKEPHALIN; DOPAMINERGIC-NEURONS; PARKINSONIAN BRAINS AB Three peptide neuromodulators that are found in high concentration in the subtantia nigra: dynorphin A 1-8, met5-enkephalin-arg6-gly7-leu8 and substance P, were measured by specific radioimmunoassays in nigral tissue from normals and schizophrenics postmortem. Substance P and dynorphin were unchanged between the two groups. However, the proenkephalin-derived peptide was significantly elevated in the schizophrenic group. The immunoreactivity was identified as authentic met5-enkephalin-arg6-gly7-leu8 by high pressure liquid chromatography. The data suggest that a different set of regulatory controls exists for nigral enkephalin peptides as compared to dynorphin and substance P, and that the former system may be disordered in schizophrenia. C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. RP IADAROLA, MJ (reprint author), NIDA,NEUROBIOL & ANESTHESIOL BRANCH,BETHESDA,MD 20892, USA. NR 58 TC 21 Z9 21 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 48 IS 20 BP 1919 EP 1930 DI 10.1016/0024-3205(91)90224-Y PG 12 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA FG119 UT WOS:A1991FG11900005 PM 1708847 ER PT J AU LONG, RM CALABRESE, MR MARTIN, BM POHL, LR AF LONG, RM CALABRESE, MR MARTIN, BM POHL, LR TI CLONING AND SEQUENCING OF A HUMAN LIVER CARBOXYLESTERASE ISOENZYME SO LIFE SCIENCES LA English DT Note ID RAT-LIVER; ANTIBODIES; HALOTHANE; HEPATITIS; ESTERASE; SERA; CDNA AB A human liver lambda gtll library was screened with antibodies raised to a purified rat liver carboxylesterase, and several clones were isolated and sequenced. The longest cDNA contained an open reading frame of 507 amino acids that represented 92% of the sequence of a mature carboxylesterase protein. This sequence possessed many structural features that are highly conserved among rabbit and rat liver carboxylesterase proteins, including Ser, His, and Asp residues that comprise the active site, two pairs of Cys residues that may participate in disulfide bond formation, and one Asn-Xxx-Thr site for N-linked carbohydrate addition. When the clone was used to probe human liver genomic DNA that had been digested with various restriction enzymes, many hybridizing bands of differing intensities were observed. The results suggest that the carboxylesterases exist as several isoenzymes in humans, and that they are encoded by multiple genes. C1 NIMH,CLIN NEUROSCI BRANCH,BETHESDA,MD 20892. NHLBI,CHEM PHARMACOL LAB,BETHESDA,MD 20892. RP LONG, RM (reprint author), UNIV MARYLAND,SCH PHARM,DEPT PHARMACOL & TOXICOL,COLLEGE PK,MD 20742, USA. NR 19 TC 16 Z9 18 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 48 IS 11 BP PL43 EP PL49 DI 10.1016/0024-3205(91)90515-D PG 7 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA EY110 UT WOS:A1991EY11000012 PM 1997784 ER PT J AU ROTHMAN, RB XU, H SEGGEL, M JACOBSON, AE RICE, KC BRINE, GA CARROLL, FI AF ROTHMAN, RB XU, H SEGGEL, M JACOBSON, AE RICE, KC BRINE, GA CARROLL, FI TI RTI-4614-4 - AN ANALOG OF (+)-CIS-3-METHYLFENTANYL WITH A 27,000-FOLD BINDING SELECTIVITY FOR MU-OPIOID VERSUS DELTA-OPIOID BINDING-SITES SO LIFE SCIENCES LA English DT Article ID OPIATE RECEPTOR; SYNTHETIC ANALGESICS; FENTANYL DERIVATIVES; RAT-BRAIN; MORPHINE; AGONIST; LIGANDS AB The objective of this study was to determine the binding affinities of (+/-)-cis-N-[1-(2-hydroxy-2-phenylethyl)-3-methyl-4-piperidyl]-N-phenylpropanamide-HCl (RTl-4614-4), which is an analog of (+)-cis-3-methylfentanyl for opioid receptor subtypes. The Ki values (nM) of this agent for opioid receptor subtypes were as follows: mu (0.0055), delta (148), kappa1 (84.8), kappa2a (2275), and kappa2b (22.3). The selectivity of this agent for the mu binding site was 27,000 vs. the delta binding site, 15,400 vs. the kappa1 binding site, 413,700 vs the kappa2a and 4,054 vs the kappa2b binding site. In contrast, two other fentanyl analogs, N-(2-(4-methylpyridinyl))-N-(1-phenethyl-4-piperidinyl)2-furamide and N-(2-pyrazinyl)-N-(1-phenethyl-4-piperdinyl)2-furamide had considerably higher Ki values at, and were less selective for, the mu binding site. Since RTl-4614-4 is composed of a mixture of four stereoisomers, the resolution of these isomers should permit identification of an extremely potent and selective agent for the opioid mu receptor. C1 NIDDK,MED CHEM LAB,RECEPTOR STUDIES UNIT,BETHESDA,MD 20892. LAB MED CHEM,DRUG DESIGN & SYNTHESIS SECT,BETHESDA,MD 20892. RES TRIANGLE INST,RES TRIANGLE PK,NC 27709. NR 26 TC 25 Z9 25 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 48 IS 23 BP PL111 EP PL116 PG 6 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA FK002 UT WOS:A1991FK00200014 ER PT J AU WITKIN, JM TORTELLA, FC AF WITKIN, JM TORTELLA, FC TI MODULATORS OF N-METHYL-D-ASPARTATE PROTECT AGAINST DIAZEPAM-RESISTANT OR PHENOBARBITAL-RESISTANT COCAINE CONVULSIONS SO LIFE SCIENCES LA English DT Note ID PHENCYCLIDINE-LIKE DRUGS; ANTICONVULSANT PROPERTIES; NMDA RECEPTORS; ACID; RATS; DEXTROMETHORPHAN; COMPLICATIONS; ANTAGONISTS; ABUSE AB The anticonvulsants diazepam (1-10 mg/kg) and phenobarbital (30-100 mg/kg) protected against lethality without altering clonic convulsions induced by 75 mg/kg cocaine (CD100) in male Swiss Webster mice. In contrast, the non-competitive N-methyl-D-aspartate (NMDA) antagonists, MK-801 (dizocilpine) and phencyclidine, produced dose-dependent protection against cocaine convulsions. The competitive NMDA antagonists, CPP and NPC 12626, were also anti- convulsant, without producing the behavioral disturbances associated with non-competitive antagonists. Diazepam and phenobarbital protected against convulsions induced by 60 mg/kg cocaine (90% convulsions alone). Compounds that act at the strychnine-insensitive glycine receptor of the NMDA receptor complex, ACPC and 7-chlorokynurinic acid, also protected against convulsions induced by 60 mg/kg cocaine. In contrast, the non-opioid antitussive anticonvulsants (dextromethorphan, caramiphen, and carbetapentane) were not active against either dose of cocaine. The efficacy of compounds as antagonists of the convulsant effects of cocaine and NMDA appear related. These results suggest a potential role for the NMDA receptor complex in the convulsant actions of cocaine and new molecular targets for drug discovery in treating cocaine toxicity. C1 WALTER REED ARMY MED CTR,NEUROPHARMACOL BRANCH,WASHINGTON,DC 20307. RP WITKIN, JM (reprint author), NIDA,ADDICT RES CTR,PSYCHOBIOL LAB,DRUG DEV GRP,POB 5180,BALTIMORE,MD 21224, USA. NR 26 TC 44 Z9 44 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PY 1991 VL 48 IS 11 BP PL51 EP PL56 DI 10.1016/0024-3205(91)90516-E PG 6 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA EY110 UT WOS:A1991EY11000013 PM 1847729 ER PT J AU ORDIDGE, RJ KNIGHT, RA HELPERN, JA AF ORDIDGE, RJ KNIGHT, RA HELPERN, JA TI MAGNETIZATION TRANSFER CONTRAST (MTC) IN FLASH MR IMAGING SO MAGNETIC RESONANCE IMAGING LA English DT Article DE MR IMAGING; MAGNETIZATION TRANSFER CONTRAST; HIGH-SPEED FLASH IMAGING AB Magnetization transfer between bound and free protons was used as a source of contrast in high speed MR imaging using the FLASH technique. Contrast in FLASH MR images was found to depend upon the reduced magnetization and the spin lattice relaxation rate of free protons in the presence of bound proton radio-frequency saturation. MTC FLASH imaging was thus used to estimate the variation with saturation frequency of free proton spin-lattice relaxation during magnetization transfer. RP ORDIDGE, RJ (reprint author), HENRY FORD HOSP,DEPT NEUROL,NIH,CTR CEREBROVASC DIS RES,2799 W GRAND BLVD,DETROIT,MI 48202, USA. RI Ordidge, Roger/F-2755-2010; OI Ordidge, Roger/0000-0002-1005-3654 FU NINDS NIH HHS [NS23393] NR 0 TC 13 Z9 13 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0730-725X J9 MAGN RESON IMAGING JI Magn. Reson. Imaging PY 1991 VL 9 IS 6 BP 889 EP 893 DI 10.1016/0730-725X(91)90532-Q PG 5 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA GV753 UT WOS:A1991GV75300001 PM 1766312 ER PT J AU ORDIDGE, RJ HELPERN, JA KNIGHT, RA QING, ZG WELCH, KMA AF ORDIDGE, RJ HELPERN, JA KNIGHT, RA QING, ZG WELCH, KMA TI INVESTIGATION OF CEREBRAL-ISCHEMIA USING MAGNETIZATION TRANSFER CONTRAST (MTC) MR IMAGING SO MAGNETIC RESONANCE IMAGING LA English DT Article DE MR IMAGING; ISCHEMIA; MAGNETIZATION TRANSFER CONTRAST AB The effects of cerebral ischemia in rat brain were monitored as a function of time using proton MR imaging. Spin-spin relaxation time (T2), proton density, and magnetization transfer contrast (MTC) were measured by MR imaging at various time intervals during a 1-week period following the induction of ischemic damage. Ischemic injury was characterized by a maximization of both T2 value and MTC appearance at 24 hr postischemic injury. These changes were accompanied by a gradual increase in MR observable water density over the first few days of ischemia. A reduction in the magnetization exchange rate between "free" and "bound" water protons as measured by MTC imaging is at least partially responsible for the elevation in T2 values observed during ischemia, and may accompany breakdown of cellular structure. RP ORDIDGE, RJ (reprint author), HENRY FORD HOSP,DEPT NEUROL,NIH,CTR CEREBROVASC DIS RES,2799 W GRAND BLVD,DETROIT,MI 48202, USA. RI Ordidge, Roger/F-2755-2010; OI Ordidge, Roger/0000-0002-1005-3654 FU NINDS NIH HHS [NS23393] NR 0 TC 48 Z9 49 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0730-725X J9 MAGN RESON IMAGING JI Magn. Reson. Imaging PY 1991 VL 9 IS 6 BP 895 EP 902 DI 10.1016/0730-725X(91)90533-R PG 8 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA GV753 UT WOS:A1991GV75300002 PM 1766313 ER PT B AU EDDY, EM OBRIEN, DA FENDERSON, BA WELCH, JE AF EDDY, EM OBRIEN, DA FENDERSON, BA WELCH, JE BE ROBAIRE, B TI INTERMEDIATE FILAMENT-LIKE PROTEINS IN THE FIBROUS SHEATH OF THE MOUSE SPERM FLAGELLUM SO MALE GERM CELL : SPERMATOGONIUM TO FERTILIZATION SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT 11TH NORTH AMERICAN TESTS WORKSHOP ON THE MALE GERM CELL : SPERMATOGONIUM TO FERTILIZATION CY APR 24-27, 1991 CL MCGILL UNIV, MONTREAL, CANADA SP NEW YORK ACAD SCI, FONDS RECH SANTE QUEBEC, MRC CANADA, NICHHD, GENETICS & IVF INST, HOFFMANN LA ROCHE, MERCK FROSST CANADA, PHARMASCIENCE, R W JOHNSON PHARM RES INST, SERONO CANADA HO MCGILL UNIV RP EDDY, EM (reprint author), NIEHS,REPROD & DEV TOXICOL LAB,GAMETE BIOL SECT,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 29 Z9 30 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-710-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 637 BP 224 EP 239 DI 10.1111/j.1749-6632.1991.tb27312.x PG 16 WC Andrology; Physiology; Reproductive Biology SC Endocrinology & Metabolism; Physiology; Reproductive Biology GA BW41F UT WOS:A1991BW41F00016 PM 1723852 ER PT J AU SLATTERY, JP VRIJENHOEK, RC LUTZ, RA AF SLATTERY, JP VRIJENHOEK, RC LUTZ, RA TI HETEROZYGOSITY, GROWTH, AND SURVIVAL OF THE HARD CLAM, MERCENARIA-MERCENARIA, IN SEAGRASS VS SANDFLAT HABITATS SO MARINE BIOLOGY LA English DT Article ID OYSTER CRASSOSTREA-VIRGINICA; MYTILUS-EDULIS; AMERICAN OYSTER; ENZYME HETEROZYGOSITY; POSSIBLE EXPLANATIONS; POPULATION-STRUCTURE; GENETIC-VARIATION; NATURAL-POPULATIONS; MULINIA-LATERALIS; COOT CLAM AB Populations of the hard clam, Mercenaria mercenaria L., sampled from dissimilar, adjacent habitats (October and November 1987), were used to assess environmental effects on associations among multi-locus heterozygosity, growth, and survival. Individuals were collected from three widely separated localities along the east coast of North America with each consisting of a seagrass bed and an adjacent, unvegetated sandflat. Demographic differences between adjacent populations were attributed to habitat type. Samples from intertidal sandflats at two of the localities were dominated by younger individuals than those from seagrass beds. Differential growth between adjacent populations was detected at only one locality wherein seagrass individuals grew faster than those from the sandflat. Allelic frequencies revealed adjacent populations that were genetically homogeneous. Nearly all of the genetic variance (98%) was within populations, yet a small (1.7%), but significant, portion occurred between localities. Observed and expected heterozygosities revealed a deficiency of heterozygotes in all six samples. Inbreeding and small-scale population subdivision were discounted as causative factors because deficiencies were heterogeneous across loci. Multi-locus heterozygosity was not correlated with growth rate in samples from any locality. Using a consensus test, multilocus heterozygosity was positively associated with ageclass in sandflat, but not seagrass, samples. We suggest that heterozygosity-fitness trait associations in marine bivalves are more likely to occur in populations inhabiting more stressful, fluctuating environments. C1 RUTGERS STATE UNIV,CTR THEORET & APPL GENET,POB 231,NEW BRUNSWICK,NJ 08903. RUTGERS STATE UNIV,DEPT MARINE & CONSTAL SCI,NEW BRUNSWICK,NJ 08903. NCI,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702. RP VRIJENHOEK, RC (reprint author), RUTGERS STATE UNIV,CTR THEORET & APPL GENET,POB 231,NEW BRUNSWICK,NJ 08903, USA. NR 61 TC 13 Z9 13 U1 1 U2 8 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0025-3162 J9 MAR BIOL JI Mar. Biol. PY 1991 VL 111 IS 3 BP 335 EP 342 DI 10.1007/BF01319404 PG 8 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA GV587 UT WOS:A1991GV58700002 ER PT J AU GAUBATZ, JW ARCEMENT, B CUTLER, RG AF GAUBATZ, JW ARCEMENT, B CUTLER, RG TI GENE-EXPRESSION OF AN ENDOGENOUS RETROVIRUS-LIKE ELEMENT DURING MURINE DEVELOPMENT AND AGING SO MECHANISMS OF AGEING AND DEVELOPMENT LA English DT Article DE INTRACISTERNAL A-PARTICLE; GENE EXPRESSION; RETROVIRUS-LIKE ELEMENTS; AGING; DEVELOPMENT; MOUSE TISSUES ID A-PARTICLE GENES; EARLY MOUSE EMBRYOS; DNA METHYLATION; C57BL-6J MICE; CELL-LINES; RNA; AGE; VIRUS; LIVER; SEQUENCE AB We have measured intracisternal A-particle (IAP) RNA levels during development and aging in C57BL/6J mouse tissues to determine possible age-dependent changes in gene expression of these retrovirus-like sequences. Total RNA was isolated from tissues of embryonic and new born mice and mice ranging in age from 2 months to 32 months of age. RNA samples were either slot-blotted directly or fractionated on denaturing agarose gels and transferred to nylon membranes. Hybridization with cloned, P-32-labeled IAP sequences showed that both the mass amounts and the relative proportions of IAP transcripts varied between tissues and as a function of age. IAP gene products were higher in brain kidney tissues than in liver and heart tissues. The relative proportion of transcripts increased in embryonic tissues until birth and following birth, was highest in neonatal or 2-month-old tissues. The adult levels of IAP-related RNAs did not change significantly from 6 to 24 months of age. However, 32-month-old tissues exhibited the lowest content of IAP transcripts, with the exception of heart tissue which did not change with age. A 5.4-kb RNa was the predominant IAP transcript in most samples, but each tissue had a characteristic size distribution of IAP-related transcripts. These results demonstrate that transcription of IAP genes continues throughout the life span of this mouse strain with tissue-specific and age-dependent regulation of expression. C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. RP GAUBATZ, JW (reprint author), UNIV SO ALABAMA,COLL MED,DEPT BIOCHEM,MOBILE,AL 36688, USA. FU NIA NIH HHS [AG07860] NR 43 TC 15 Z9 15 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0047-6374 J9 MECH AGEING DEV JI Mech. Ageing. Dev. PD JAN PY 1991 VL 57 IS 1 BP 71 EP 85 DI 10.1016/0047-6374(91)90025-U PG 15 WC Cell Biology; Geriatrics & Gerontology SC Cell Biology; Geriatrics & Gerontology GA EQ278 UT WOS:A1991EQ27800006 PM 1900553 ER PT J AU WARBURG, A MONTOYALERMA, J JARAMILLO, C CRUZRUIZ, AL OSTROVSKA, K AF WARBURG, A MONTOYALERMA, J JARAMILLO, C CRUZRUIZ, AL OSTROVSKA, K TI LEISHMANIASIS VECTOR POTENTIAL OF LUTZOMYIA SPP IN COLOMBIAN COFFEE PLANTATIONS SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Article DE LUTZOMYIA-COLUMBIANA; LUTZOMYIA-TOWNSENDI; LUTZOMYIA SPP; LEISHMANIA-BRAZILIENSIS; AUTOGENY; PARITY; VECTOR COMPETENCE; COFFEE PLANTATIONS; COLOMBIA AB Potential vectors of Leishmania braziliensis Vianna were assessed at four study sites in the mountainous Valle del Cauca, western Colombia, from March to June 1989. In an active focus of transmission at 1450 m altitude, a coffee plantation at Versalles, there were high densities of anthropophilic phlebotomines: Lutzomyia columbiana (Ritorcelli & Van Ty) and Lu.townsendi (Oritz), both in the verrucarum species group, and of Lu.pia (Fairchild & Hertig). At a comparable altitude in a forest reserve at Yotoco where leishmaniasis is unknown, Lu.pia was the prevalent species and Lu.townsendi was absent. In two localities at 1150 m altitude, there were plentiful Lu.lichyi (Floch & Abonnenc) plus both species in the verrucarum group, but Lu.pia was absent. One of these localities, a coffee plantation at Villa Hermosa where a leishmaniasis outbreak occurred in 1986, was compared with a leishmaniasis-free, partly wooded nature reserve at Mateguadua. No natural infections of Leishmania were found in a total of 1896 wild-caught female phlebotomines belonging to at least seven species. It remains unclear why Leishmaniasis transmission is associated with coffee plantations in this part of Colombia. Laboratory-bred Lu.lichyi females were invariably autogenous, and blood-seeking females of this species were always parous. Parity rates in wild-caught females of other species were 55% Lu.pia, 24% Lu.columbiana and 14% Lu.townsendi. Female Lutzomyia infected artificially with Le.braziliensis promastigotes developed peripylarian infections. Higher proportions of Lu.townsendi (96%) and Lu.columbiana (78%) became infected but these species developed lower rates of stomodaeal infections (P < 0.1) than Lu.lichyi (37%) or Lu.pia (44%). Only 33% of a Colombian strain of Lu.longipalpis (Lutz & Neiva) became infected. Combined evidence, from these laboratory and field studies, indicates that the sandfly species most strongly associated with leishmaniasis transmission in Colombian coffee plantations are Lu.columbiana and Lu.townsendi. Further work is required to clarify the local status of the other potential vector phlebotomines. RP WARBURG, A (reprint author), NIAID,PARASIT DIS LAB,BETHESDA,MD 20892, USA. OI Montoya-Lerma, James/0000-0003-2122-1323 NR 0 TC 26 Z9 27 U1 0 U2 3 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0269-283X J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD JAN PY 1991 VL 5 IS 1 BP 9 EP 16 DI 10.1111/j.1365-2915.1991.tb00514.x PG 8 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA EZ658 UT WOS:A1991EZ65800002 PM 1768906 ER PT J AU BAKER, MS KESSLER, LG URBAN, N SMUCKER, RC AF BAKER, MS KESSLER, LG URBAN, N SMUCKER, RC TI ESTIMATING THE TREATMENT COSTS OF BREAST AND LUNG-CANCER SO MEDICAL CARE LA English DT Article ID DISEASE; WOMEN AB The Continuous Medicare History Sample File (CMHSF) was used to derive an estimate of the lifetime direct medical expenses attributable to two chronic diseases, lung cancer and female breast cancer. These two cancers are the leading cancer causes of death in men and women in the United States. They inflict large costs on the population, both direct and indirect, but the costs have been difficult to measure. The primary obstacle to quantification is the intermittent and long-term nature of treatment for these diseases. A complete record of expenses cannot usually be obtained from one source, however, a review of all the national health surveys, as well as the Medicare statistical files identified the CMHSF, which is maintained by the Health Care Financing Administration in a format suitable for calculation of cumulative medical expenses. Some of the pertinent features of the CMHSF include the following: 1) it is a nationally representative sample of the Medicare population, 2) it is longitudinal covering an 8-year period from 1974 to 1981, 3) it captures the majority of medical expenses for each enrollee, and 4) it can be linked to other national data bases such as the National Death Index. Charges for three phases of cancer treatment were derived from the file: initial therapy, maintenance care, and terminal care. A method is described for computing the present value of lifetime treatment costs from the phase-specific charges. The lifetime cost of treating breast cancer in 1984 dollars is $36,926 and lung cancer is $12,510. C1 NCI,DIV CANC PREVENT & CONTROL,BETHESDA,MD 20892. INFORMAT MANAGEMENT SERV INC,SILVER SPRING,MD. RP BAKER, MS (reprint author), FRED HUTCHINSON CANC RES CTR,1124 COLUMBIA ST,SEATTLE,WA 98104, USA. NR 24 TC 91 Z9 91 U1 1 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD JAN PY 1991 VL 29 IS 1 BP 40 EP 49 DI 10.1097/00005650-199101000-00004 PG 10 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA ET624 UT WOS:A1991ET62400004 PM 1986176 ER PT J AU DAWID, IB AF DAWID, IB TI MESODERM INDUCTION SO METHODS IN CELL BIOLOGY LA English DT Review ID FIBROBLAST GROWTH-FACTOR; EARLY XENOPUS EMBRYOS; ERYTHROID-DIFFERENTIATION FACTOR; HOMEOBOX GENE XHOX3; CARDIAC ACTIN GENE; MESSENGER-RNA; PATTERN-FORMATION; LAEVIS EMBRYOS; FROG EMBRYOS; CELL-LINE C1 NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. NR 71 TC 14 Z9 14 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0091-679X J9 METHOD CELL BIOL JI Methods Cell Biol. PY 1991 VL 36 BP 311 EP 328 PG 18 WC Cell Biology SC Cell Biology GA MC414 UT WOS:A1991MC41400019 PM 1811141 ER PT J AU SARGENT, TD MATHERS, PH AF SARGENT, TD MATHERS, PH TI ANALYSIS OF CLASS-II GENE-REGULATION SO METHODS IN CELL BIOLOGY LA English DT Review ID XENOPUS-LAEVIS; EMBRYOS; EXPRESSION; TRANSCRIPTION; ENHANCER; EGGS; CARG C1 NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. NR 16 TC 7 Z9 7 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0091-679X J9 METHOD CELL BIOL JI Methods Cell Biol. PY 1991 VL 36 BP 347 EP & PG 0 WC Cell Biology SC Cell Biology GA MC414 UT WOS:A1991MC41400021 PM 1811143 ER PT J AU WOLFFE, AP SCHILD, C AF WOLFFE, AP SCHILD, C TI CHROMATIN ASSEMBLY SO METHODS IN CELL BIOLOGY LA English DT Review ID XENOPUS-LAEVIS OOCYTES; TRANSCRIPTION FACTOR-IIIA; DNA-REPLICATION INVITRO; HISTONE SYNTHESIS; NUCLEOSOME; NUCLEOPLASMIN; EXTRACTS; COMPLEX; INITIATION; PROTEIN C1 NIDDKD,MOLEC BIOL LAB,BETHESDA,MD 20892. UNIV LAUSANNE,CH-1015 LAUSANNE,SWITZERLAND. RI Schild-Poulter, Caroline/K-4476-2013 NR 53 TC 16 Z9 16 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0091-679X J9 METHOD CELL BIOL JI Methods Cell Biol. PY 1991 VL 36 BP 541 EP 559 PG 19 WC Cell Biology SC Cell Biology GA MC414 UT WOS:A1991MC41400030 PM 1811151 ER PT J AU ROTH, SY SIMPSON, RT AF ROTH, SY SIMPSON, RT TI YEAST MINICHROMOSOMES SO METHODS IN CELL BIOLOGY LA English DT Review ID DETERMINE NUCLEOSOME POSITIONS; NUCLEASE-SENSITIVE REGIONS; PROTEIN-DNA INTERACTIONS; SACCHAROMYCES-CEREVISIAE; CHROMATIN STRUCTURE; PLASMID CHROMATIN; SEQUENCE; UPSTREAM; CLEAVAGE; PROMOTER C1 NIDDKD,CELLULAR & DEV BIOL LAB,BETHESDA,MD 20892. NR 46 TC 18 Z9 18 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0091-679X J9 METHOD CELL BIOL JI Methods Cell Biol. PY 1991 VL 35 BP 289 EP 314 PG 26 WC Cell Biology SC Cell Biology GA MC413 UT WOS:A1991MC41300012 PM 1779859 ER PT J AU HULL, MW THOMAS, G HUIBREGTSE, JM ENGELKE, DR AF HULL, MW THOMAS, G HUIBREGTSE, JM ENGELKE, DR TI PROTEIN-DNA INTERACTIONS IN-VIVO - EXAMINING GENES IN SACCHAROMYCES-CEREVISIAE AND DROSOPHILA-MELANOGASTER BY CHROMATIN FOOTPRINTING SO METHODS IN CELL BIOLOGY LA English DT Review ID TRANSFER-RNA GENE; I-HYPERSENSITIVE SITES; LIGATION MEDIATED PCR; HEAT-SHOCK GENES; TRANSCRIPTION COMPLEXES; METHYLATION ANALYSIS; 5'-FLANKING REGION; POLYMERASE-III; X-LAEVIS; PROMOTER C1 UNIV MICHIGAN,DEPT BIOL SCI,ANN ARBOR,MI 48109. HARVARD UNIV,DEPT CELLULAR & DEV BIOL,CAMBRIDGE,MA 02138. NCI,TUMOR VIRUS BIOL,BETHESDA,MD 20892. FU NIGMS NIH HHS [GM31532] NR 47 TC 12 Z9 13 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0091-679X J9 METHOD CELL BIOL JI Methods Cell Biol. PY 1991 VL 35 BP 383 EP 415 PG 33 WC Cell Biology SC Cell Biology GA MC413 UT WOS:A1991MC41300016 PM 1664031 ER PT J AU DIXON, WJ HAYES, JJ LEVIN, JR WEIDNER, MF DOMBROSKI, BA TULLIUS, TD AF DIXON, WJ HAYES, JJ LEVIN, JR WEIDNER, MF DOMBROSKI, BA TULLIUS, TD TI HYDROXYL RADICAL FOOTPRINTING SO METHODS IN ENZYMOLOGY LA English DT Review ID REPRESSOR-OPERATOR INTERACTIONS; LAMBDA-REPRESSOR; DNA-BINDING; GEL-ELECTROPHORESIS; PROTEIN CONTACTS; ADJACENT MAJOR; TRANSCRIPTION; RESOLUTION; REGIONS; ACCESSIBILITY C1 JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218. NIH,MOLEC BIOL LAB,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,DEPT CHEM,BALTIMORE,MD 21218. JOHNS HOPKINS MED INST,CTR MED GENET,BALTIMORE,MD 21205. RI Tullius, Thomas/A-9685-2008; Delehanty, James/F-7454-2012 OI Tullius, Thomas/0000-0003-4425-796X; FU NCI NIH HHS [CA 01208]; NIGMS NIH HHS [GM 41930] NR 42 TC 204 Z9 205 U1 1 U2 8 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Method Enzymol. PY 1991 VL 208 BP 380 EP 413 PG 34 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA MC428 UT WOS:A1991MC42800020 PM 1664026 ER PT J AU GONZALEZ, FJ AOYAMA, T GELBOIN, HV AF GONZALEZ, FJ AOYAMA, T GELBOIN, HV TI EXPRESSION OF MAMMALIAN CYTOCHROME-P450 USING VACCINIA VIRUS SO METHODS IN ENZYMOLOGY LA English DT Review ID CDNA-DIRECTED EXPRESSION; RNA-POLYMERASE; HUMAN-LIVER; SYSTEM; SEQUENCE; RECOMBINANTS; CLONING; GENES RP GONZALEZ, FJ (reprint author), NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. NR 18 TC 90 Z9 90 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Method Enzymol. PY 1991 VL 206 BP 85 EP 92 PG 8 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG720 UT WOS:A1991HG72000008 PM 1664484 ER PT J AU GONZALEZ, FJ KIMURA, S TAMURA, S GELBOIN, HV AF GONZALEZ, FJ KIMURA, S TAMURA, S GELBOIN, HV TI EXPRESSION OF MAMMALIAN CYTOCHROME-P450 USING BACULOVIRUS SO METHODS IN ENZYMOLOGY LA English DT Review ID SACCHAROMYCES-CEREVISIAE; CELLS; GENE; VIRUS; CDNA RP GONZALEZ, FJ (reprint author), NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. NR 16 TC 70 Z9 70 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Method Enzymol. PY 1991 VL 206 BP 93 EP 99 PG 7 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG720 UT WOS:A1991HG72000009 PM 1784251 ER PT J AU GUENGERICH, FP BRIAN, WR SARI, MA ROSS, JT AF GUENGERICH, FP BRIAN, WR SARI, MA ROSS, JT TI EXPRESSION OF MAMMALIAN CYTOCHROME-P450 ENZYMES USING YEAST-BASED VECTORS SO METHODS IN ENZYMOLOGY LA English DT Review ID CYTOCHROMES-P-450 LAURATE (OMEGA-1)-HYDROXYLASE; SITE-DIRECTED MUTAGENESIS; GENETICALLY ENGINEERED P450-MONOOXYGENASES; NADPH-CYTOCHROME-P-450 REDUCTASE CDNA; FUNCTIONAL-MOUSE CYTOCHROME-P-450-P1; TOLBUTAMIDE HYDROXYLASE-ACTIVITY; MODIFY SUBSTRATE-SPECIFICITY; RAT-LIVER CYTOCHROME-P-450; INDUCIBLE CUP1 PROMOTER; TREATED RABBIT LIVER C1 SMITH KLINE BEECHAM PHARMACEUT,KING OF PRUSSIA,PA 19406. UNIV PARIS 05,CHIM & BIOCHIM PHARMACOL & TOXICOL LAB,CNRS,F-75720 PARIS,FRANCE. NCI,FREDERICK CANC RES FACIL,PROGRAM RESOURCES INC,FERMENTAT PROD FACIL,FREDERICK,MD 21701. RP GUENGERICH, FP (reprint author), VANDERBILT UNIV,MED CTR,SCH MED,DEPT BIOCHEM,NASHVILLE,TN 37232, USA. NR 48 TC 63 Z9 63 U1 3 U2 5 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Method Enzymol. PY 1991 VL 206 BP 130 EP 145 PG 16 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG720 UT WOS:A1991HG72000014 PM 1784205 ER PT J AU NEGISHI, M BURKHART, B AIDA, K AF NEGISHI, M BURKHART, B AIDA, K TI EXPRESSION OF GENES WITHIN MOUSE IIA AND IID SUBFAMILIES - SIMULTANEOUS MEASUREMENT OF HOMOLOGOUS P450 MESSENGER-RNAS SO METHODS IN ENZYMOLOGY LA English DT Review ID STEROID 15-ALPHA-HYDROXYLASE; FAMILY; P-45015-ALPHA RP NEGISHI, M (reprint author), NIEHS,REPROD & DEV TOXICOL LAB,PHARMACOGENET SECT,RES TRIANGLE PK,NC 27709, USA. NR 9 TC 10 Z9 10 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Method Enzymol. PY 1991 VL 206 BP 267 EP 273 PG 7 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG720 UT WOS:A1991HG72000025 PM 1784213 ER PT J AU PHILPOT, RM AF PHILPOT, RM TI CHARACTERIZATION OF CYTOCHROME-P450 IN EXTRAHEPATIC TISSUES SO METHODS IN ENZYMOLOGY LA English DT Review ID MONOOXYGENASE SYSTEM RP PHILPOT, RM (reprint author), NIEHS,CELLULAR & MOLEC PHARMACOL,RES TRIANGLE PK,NC 27709, USA. NR 9 TC 18 Z9 18 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Method Enzymol. PY 1991 VL 206 BP 623 EP 631 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG720 UT WOS:A1991HG72000061 PM 1784245 ER PT J AU KUNKEL, TA BEBENEK, K MCCLARY, J AF KUNKEL, TA BEBENEK, K MCCLARY, J TI EFFICIENT SITE-DIRECTED MUTAGENESIS USING URACIL-CONTAINING DNA SO METHODS IN ENZYMOLOGY LA English DT Review ID BACTERIOPHAGE-T7 DEOXYRIBONUCLEIC-ACID; INVITRO MUTAGENESIS; STRAND DISPLACEMENT; POLYMERASE; REPLICATION; TEMPLATE; PROTEIN C1 CODEN, San Francisco, CA 94080 USA. RP NIEHS, MOLEC GENET LAB, RES TRIANGLE PK, NC 27709 USA. NR 20 TC 9 Z9 9 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 204 BP 125 EP 139 PG 15 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GN469 UT WOS:A1991GN46900006 ER PT J AU PADLAN, EA KABAT, EA AF PADLAN, EA KABAT, EA TI MODELING OF ANTIBODY COMBINING SITES SO METHODS IN ENZYMOLOGY LA English DT Article ID ANTIGEN-BINDING-SITE; COMPLEMENTARITY-DETERMINING RESIDUES; IMMUNOGLOBULIN LIGHT-CHAINS; MOUSE MYELOMA PROTEINS; BENCE JONES PROTEINS; 3-DIMENSIONAL STRUCTURE; HYPERVARIABLE REGIONS; VARIABLE POSITIONS; CRYSTAL-STRUCTURE; 2.8-A RESOLUTION C1 COLUMBIA UNIV COLL PHYS & SURG, DEPT NEUROL, NEW YORK, NY 10032 USA. COLUMBIA UNIV COLL PHYS & SURG, DEPT MICROBIOL GENET & DEV, NEW YORK, NY 10032 USA. COLUMBIA UNIV COLL PHYS & SURG, DEPT NEUROL, NEW YORK, NY 10032 USA. RP PADLAN, EA (reprint author), NIDDKD, MOLEC BIOL LAB, BETHESDA, MD 20892 USA. NR 91 TC 2 Z9 2 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 203 BP 3 EP 21 PG 19 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG716 UT WOS:A1991HG71600001 ER PT J AU SINIGAGLIA, F ROMAGNOLI, P GUTTINGER, M TAKACS, B PINK, JRL AF SINIGAGLIA, F ROMAGNOLI, P GUTTINGER, M TAKACS, B PINK, JRL TI SELECTION OF T-CELL EPITOPES AND VACCINE ENGINEERING SO METHODS IN ENZYMOLOGY LA English DT Article ID TOXIC LYMPHOCYTES-T; CIRCUMSPOROZOITE PROTEIN; MONOCLONAL-ANTIBODIES; ANTIGEN RECOGNITION; FALCIPARUM; PEPTIDES; MOLECULES; REGIONS; STAGE C1 NIAID, IMMUNOL LAB, BETHESDA, MD 20892 USA. RP SINIGAGLIA, F (reprint author), F HOFFMANN LA ROCHE & CO LTD, PHARMA RES TECHNOL, CH-4002 BASEL, SWITZERLAND. RI Romagnoli, Paola/K-2237-2014 NR 36 TC 1 Z9 1 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 203 BP 370 EP 386 PG 17 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG716 UT WOS:A1991HG71600019 ER PT J AU GUND, TM SPIVAK, CE AF GUND, TM SPIVAK, CE TI PHARMACOPHORE FOR NICOTINIC AGONISTS SO METHODS IN ENZYMOLOGY LA English DT Article ID DRUG DESIGN; ANATOXIN-A; ACETYLCHOLINE; RECEPTORS; GEOMETRY; AID C1 NIDA, ADDICT RES CTR, BALTIMORE, MD 21224 USA. RP GUND, TM (reprint author), NEW JERSEY INST TECHNOL, DEPT CHEM CHEM ENGN & ENVIRONM SCI, NEWARK, NJ 07102 USA. NR 47 TC 0 Z9 0 U1 1 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 203 BP 677 EP 693 PG 17 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG716 UT WOS:A1991HG71600032 ER PT J AU LINDBERG, RLP NEGISHI, M AF LINDBERG, RLP NEGISHI, M TI MODULATION OF SPECIFICITY AND ACTIVITY IN MAMMALIAN CYTOCHROME-P-450 SO METHODS IN ENZYMOLOGY LA English DT Review ID AXIAL LIGAND TRANS; AMINO-ACID; LAURATE (OMEGA-1)-HYDROXYLASE; TESTOSTERONE 16-ALPHA-HYDROXYLASE; SUBSTRATE-SPECIFICITY; CRYSTAL-STRUCTURE; RAT-LIVER; SEQUENCE; MOUSE; STEREOSPECIFICITY C1 NIEHS,REPROD & DEV TOXICOL LAB,PHARMACOGENET SECT,RES TRIANGLE PK,NC 27709. RP LINDBERG, RLP (reprint author), UNIV BASEL,BIOCTR,DEPT PHARMACOL,CH-4056 BASEL,SWITZERLAND. NR 30 TC 5 Z9 5 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Method Enzymol. PY 1991 VL 202 BP 741 EP 752 DI 10.1016/0076-6879(91)02035-8 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GV013 UT WOS:A1991GV01300033 PM 1784197 ER PT J AU HUANG, KP HUANG, FL AF HUANG, KP HUANG, FL TI PURIFICATION AND ANALYSIS OF PROTEIN-KINASE-C ISOZYMES SO METHODS IN ENZYMOLOGY LA English DT Review ID RAT-BRAIN; EXPRESSION; IDENTIFICATION RP NICHHD, ENDOCRINOL & REPROD RES BRANCH, METAB REGULAT SECT, BETHESDA, MD 20892 USA. NR 15 TC 1 Z9 2 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 200 BP 241 EP 252 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GN467 UT WOS:A1991GN46700020 ER PT J AU KRAUS, MH AARONSON, SA AF KRAUS, MH AARONSON, SA TI DETECTION AND ISOLATION OF NOVEL PROTEIN-TYROSINE KINASE GENES EMPLOYING REDUCED STRINGENCY HYBRIDIZATION SO METHODS IN ENZYMOLOGY LA English DT Review ID FAMILY RP NCI, CELLULAR & MOLEC BIOL LAB, BETHESDA, MD 20892 USA. NR 9 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 200 BP 546 EP 556 PG 11 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GN467 UT WOS:A1991GN46700046 ER PT J AU LESNIAK, MA BASSAS, L ROTH, J HILL, JM AF LESNIAK, MA BASSAS, L ROTH, J HILL, JM TI AUTORADIOGRAPHIC LOCALIZATION OF INSULIN-LIKE GROWTH FACTOR-I RECEPTORS IN RAT-BRAIN AND CHICK-EMBRYO SO METHODS IN ENZYMOLOGY LA English DT Review C1 FDN PUITVERT, E-08025 BARCELONA, SPAIN. NICHHD, NEUROCHEM UNIT, BETHESDA, MD 20892 USA. RP NIDDKD, DIABET BRANCH, BETHESDA, MD 20892 USA. NR 11 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 198 BP 26 EP 35 PG 10 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GM724 UT WOS:A1991GM72400003 ER PT J AU KAGEYAMA, R MERLINO, GT AF KAGEYAMA, R MERLINO, GT TI INVITRO TRANSCRIPTION OF EPIDERMAL GROWTH-FACTOR RECEPTOR GENE SO METHODS IN ENZYMOLOGY LA English DT Review ID DNA-BINDING PROTEINS; RNA POLYMERASE-II; PROMOTER; SEQUENCE; SP1 C1 NCI, MOLEC BIOL LAB, BETHESDA, MD 20892 USA. RP KYOTO UNIV, FAC MED, INST IMMUNOL, KYOTO 606, JAPAN. NR 16 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 198 BP 242 EP 250 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GM724 UT WOS:A1991GM72400023 ER PT J AU DIFIORE, PP SEGATTO, O AARONSON, SA AF DIFIORE, PP SEGATTO, O AARONSON, SA TI CLONING, EXPRESSION, AND BIOLOGICAL EFFECTS OF ERBB-2/NEU GENE IN MAMMALIAN-CELLS SO METHODS IN ENZYMOLOGY LA English DT Review ID NEU ONCOGENE; PROTO-ONCOGENE; HUMAN-BREAST; RECEPTOR; AMPLIFICATION; NEUROBLASTOMAS; OVEREXPRESSION; TRANSFORMATION; C-ERBB-2; CANCER C1 MAX PLANCK INST BIOCHEM, DEPT MOLEC BIOL, W-8033 MARTINSRIED, GERMANY. RP NCI, CELLULAR & MOLEC BIOL LAB, BETHESDA, MD 20892 USA. RI Di Fiore, Pier Paolo/K-2130-2012 OI Di Fiore, Pier Paolo/0000-0002-2252-0950 NR 20 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 198 BP 272 EP 277 PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GM724 UT WOS:A1991GM72400026 ER PT J AU KEMPNER, ES OSBORNE, JC REYNOLDS, LJ DEEMS, RA DENNIS, EA AF KEMPNER, ES OSBORNE, JC REYNOLDS, LJ DEEMS, RA DENNIS, EA TI ANALYSIS OF LIPASES BY RADIATION INACTIVATION SO METHODS IN ENZYMOLOGY LA English DT Review ID COBRA VENOM PHOSPHOLIPASE-A2; ENCODING LIPOPROTEIN-LIPASE; MOLECULAR-WEIGHT; SUBMICELLAR CONCENTRATIONS; GENE FAMILY; AGGREGATION; SEQUENCE; CDNA; CLONING; SIZE C1 UNIV CALIF SAN DIEGO, DEPT CHEM, LA JOLLA, CA 92093 USA. BECKMAN INSTRUMENTS INC, ADV DEV UNIT, FULLERTON, CA 92634 USA. RP NIAMSD, BETHESDA, MD 20892 USA. NR 44 TC 6 Z9 6 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 197 BP 280 EP 288 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GM723 UT WOS:A1991GM72300025 ER PT J AU RHEE, SG RYU, SH LEE, KY CHO, KS AF RHEE, SG RYU, SH LEE, KY CHO, KS TI ASSAYS OF PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C AND PURIFICATION OF ISOZYMES FROM BOVINE BRAINS SO METHODS IN ENZYMOLOGY LA English DT Review ID EXOGENOUS PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; RAT-LIVER; GUANINE-NUCLEOTIDE; PLASMA-MEMBRANES; PHOSPHODIESTERASE; 4-PHOSPHATE; HYDROLYSIS; ACTIVATION; CYTOSOL; BINDING C1 POSTECH, DEPT LIFE SCI, POHANG, SOUTH KOREA. CHONAM NATL UNIV, COLL MED, DEPT BIOCHEM, KWANGJU, SOUTH KOREA. HAN YANG UNIV, COLL NATL SCI, DEPT BIOCHEM, ANSAN, SOUTH KOREA. RP NHLBI, BIOCHEM LAB, BETHESDA, MD 20892 USA. NR 24 TC 54 Z9 54 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 197 BP 502 EP 511 PG 10 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GM723 UT WOS:A1991GM72300048 ER PT J AU LYNCH, TJ BRZESKA, H BAINES, IC KORN, ED AF LYNCH, TJ BRZESKA, H BAINES, IC KORN, ED TI PURIFICATION OF MYOSIN-I AND MYOSIN-I HEAVY-CHAIN KINASE FROM ACANTHAMOEBA-CASTELLANII SO METHODS IN ENZYMOLOGY LA English DT Review ID COFACTOR PROTEIN; ACTIN ACTIVATION; 3RD ISOFORM; PHOSPHORYLATION; CHROMATOGRAPHY; NUCLEOTIDES RP NHLBI, CELL BIOL LAB, BETHESDA, MD 20892 USA. RI Korn, Edward/F-9929-2012 NR 19 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 196 BP 12 EP 23 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FN841 UT WOS:A1991FN84100003 ER PT J AU CONTI, MA ADELSTEIN, RS AF CONTI, MA ADELSTEIN, RS TI PURIFICATION AND PROPERTIES OF MYOSIN LIGHT CHAIN KINASES SO METHODS IN ENZYMOLOGY LA English DT Review ID SMOOTH-MUSCLE MYOSIN; DEPENDENT PROTEIN-KINASE; RABBIT SKELETAL-MUSCLE; AMINO-ACID SEQUENCE; CATALYTIC SUBUNIT; PHOSPHORYLATION; CALMODULIN; BINDING RP NIH, MOLEC CARDIOL LAB, BETHESDA, MD 20892 USA. NR 30 TC 0 Z9 0 U1 2 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 196 BP 34 EP 47 PG 14 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FN841 UT WOS:A1991FN84100005 ER PT J AU BUBB, MR KORN, ED AF BUBB, MR KORN, ED TI PURIFICATION OF ACTOBINDIN FROM ACANTHAMOEBA-CASTELLANII SO METHODS IN ENZYMOLOGY LA English DT Review ID PROFILIN RP NHLBI, CELL BIOL LAB, BETHESDA, MD 20892 USA. RI Korn, Edward/F-9929-2012 NR 8 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1991 VL 196 BP 119 EP 125 PG 7 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FN841 UT WOS:A1991FN84100012 ER PT J AU WALSETH, TF YUEN, PST MOOS, MC AF WALSETH, TF YUEN, PST MOOS, MC TI PREPARATION OF ALPHA-P-32-LABELED NUCLEOSIDE TRIPHOSPHATES, NICOTINAMIDE ADENINE-DINUCLEOTIDE, AND CYCLIC-NUCLEOTIDES FOR USE IN DETERMINING ADENYLYL AND GUANYLYL CYCLASES AND CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE SO METHODS IN ENZYMOLOGY LA English DT Review ID ENZYMATIC PREPARATION; EUKARYOTIC CELLS; ASSAY; TOXIN; GTP; CAT) and four in codon 12 of Ki-ras (GGT --> GAT in two and GGT --> GTT in two). No evidence was found for amplification or overexpression of the c-erbB2 or EGFR genes in any tumor. One tumor contained amplified c-myc sequences and exhibited relative overexpression of c-myc. These data suggest that the amplification or overexpression of several proto-oncogenes frequently observed in other human gynecologic and breast tumors are not prevalent in endometrial carcinoma and that ras gene mutations are relatively common in this tumor type. RP BOYD, J (reprint author), NIEHS,MOLEC CARCINOGENESIS LAB,GENE EXPRESS SECT,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 59 TC 50 Z9 50 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 3 BP 189 EP 195 DI 10.1002/mc.2940040305 PG 7 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA FV078 UT WOS:A1991FV07800004 PM 2064724 ER PT J AU HERNANDEZ, L PETROPOULOS, CJ HUGHES, SH LIJINSKY, W AF HERNANDEZ, L PETROPOULOS, CJ HUGHES, SH LIJINSKY, W TI DNA METHYLATION AND ONCOGENE EXPRESSION IN METHAPYRILENE-INDUCED RAT-LIVER TUMORS AND IN TREATED HEPATOCYTES IN CULTURE SO MOLECULAR CARCINOGENESIS LA English DT Article DE GENE EXPRESSION; CARCINOGENESIS; ALKYLATION ID RAT SERUM-ALBUMIN; MOLECULAR-CLONING; SARCOMA-VIRUS; CARCINOGEN METHAPYRILENE; S-ADENOSYLHOMOCYSTEINE; LIVER CARCINOGENESIS; EPITHELIAL-CELLS; PROTO-ONCOGENE; H-RAS; C-MYC AB Continued exposure of rats to carcinogenic doses of methapyrilene (MP) leads to elevated levels of 5-methyl-deoxycytidine (5MC) in liver DNA. Since gene expression often correlates with DNA methylation, we investigated these parameters in the MP-induced hepatocellular carcinomas of Fischer 344 rats. DNA was hypermethylated in liver tissue surrounding the tumors relative to liver tissue of untreated controls of the same age, while tumor DNA was not; DNA methylation declined to normal levels when MP treatment ceased. Gene expression analysis showed measurable levels of mRNA for c-Ki-ras, erb-B, erb-B2, hck, src, lyn, vav, trk, raf-1, l-myc, c-jun, c-yes, c-myc, c-abl, and p53. No significant differences in expression for these and other oncogenes were seen between tumors and surrounding livers, although erb-B2 and vav showed visible decreases compared with normal liver. Hypermethylation of DNA and expression of these oncogenes in MP-treated tissues were not correlated. Levels of mRNA for the same genes in MP-treated hepatocytes in culture were similar to in vivo levels; analysis of DNA synthesis levels showed that this gene expression pattern occurred in the absence of proliferation bursts or toxicity in these cells, thus suggesting that treatment in vivo may produce the same results. C1 NCI,FREDERICK CANC RES & DEV CTR,MOLEC MECHANISMS CARCINOGENESIS LAB,FREDERICK,MD 21701. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74101] NR 59 TC 7 Z9 7 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 3 BP 203 EP 209 DI 10.1002/mc.2940040307 PG 7 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA FV078 UT WOS:A1991FV07800006 PM 2064726 ER PT J AU GLICK, AB SPORN, MB YUSPA, SH AF GLICK, AB SPORN, MB YUSPA, SH TI ALTERED REGULATION OF TGF-BETA-1 AND TGF-ALPHA IN PRIMARY KERATINOCYTES AND PAPILLOMAS EXPRESSING V-HA-RAS SO MOLECULAR CARCINOGENESIS LA English DT Article DE DIFFERENTIATION; GENE EXPRESSION; KERATINOCYTE; RAS ONCOGENE; TGF-ALPHA, TGF-BETA ID TRANSFORMING GROWTH-FACTOR; MOUSE EPIDERMAL-CELLS; SERUM-FREE MEDIUM; FACTOR-BETA; TERMINAL DIFFERENTIATION; SKIN CARCINOGENESIS; SARCOMA-VIRUSES; MESSENGER-RNAS; RECEPTOR; AUTOCRINE AB The influence of an oncogenic v-Ha-ras gene on the expression of TGF-beta and TGF-alpha by mouse keratinocytes and derived tumors has been investigated. Normal mouse keratinocytes cultured as basal cells in 0.05 mM Ca2+ secreted low levels of TGF-beta-2 peptide, and this increased markedly following culture in 1.4 mM Ca2+, retinoic acid, or phorbol esters. In contrast, introduction of a v-Ha-ras gene into normal keratinocytes increased basal expression and secretion of TGF-beta-1 (rather than TGF-beta-2) in response to all three agents. The selective secretion of TGF-beta-1 in v-Ha-ras keratinocytes in response to 1.4 mM Ca2+ occurred even though the four TGF-beta-2 transcripts were induced and the TGF-beta-1 transcript decreased, suggesting that the activated v-Ha-ras gene product regulates expression of the TGF-beta isoforms at the posttranscriptional level. Immunohistochemical analysis of papillomas formed following skin grafting of v-Ha-ras keratinocytes onto nude mice indicated that TGF-beta-1 was abundant in the basal and spinous layers, while there was no expression of TGF-beta-1 in normal skin. In contrast, both normal and neoplastic tissues expressed TGF-beta-2 and TGF-beta-3 in the granular layers. Furthermore, TGF-alpha mRNA expression was also elevated fivefold in cultured v-Ha-ras keratinocytes, and TGF-alpha protein was overexpressed in the grafted papillomas, but there was no detectable expression in normal skin. Elevated expression of both TGF-beta-1 and TGF-alpha in the basal and spinous layers of benign tumors may be important for the high proliferation rate in these tumors as well as for increased proliferation in the suprabasal layer. C1 NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. RP GLICK, AB (reprint author), NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BLDG 37,ROOM 3B25,BETHESDA,MD 20892, USA. NR 39 TC 93 Z9 94 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 3 BP 210 EP 219 DI 10.1002/mc.2940040308 PG 10 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA FV078 UT WOS:A1991FV07800007 PM 2064727 ER PT J AU WESTON, A VINEIS, P CAPORASO, NE KRONTIRIS, TG LONERGAN, JA SUGIMURA, H AF WESTON, A VINEIS, P CAPORASO, NE KRONTIRIS, TG LONERGAN, JA SUGIMURA, H TI RACIAL VARIATION IN THE DISTRIBUTION OF HA-RAS-1 ALLELES SO MOLECULAR CARCINOGENESIS LA English DT Article DE RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; VARIABLE TANDEM REPEATS; ONCOGENE; EPIDEMIOLOGY; CARCINOGENESIS ID RARE HA-RAS; FRAGMENT LENGTH POLYMORPHISMS; CANCER-PATIENTS; HUMAN-LUNG; HYPERVARIABLE ALLELES; RISK ASSESSMENT; SEQUENCES; MYELODYSPLASIA; CHROMOSOME-11; ASSOCIATION AB Restriction fragment length polymorphism analyses of the Ha-ras-1 proto-oncogene were undertaken in white and black populations residing in the Baltimore-Washington metropolitan area to address whether specific rare alleles of the Ha-ras-1 proto-oncogene locus vary in their distribution among different racial groups. High-molecular-weight genomic DNA samples from the lungs of 80 lung cancer patients and 92 accident victims were digested with appropriate restriction enzymes and subjected to Southern analysis using the 6.6-kb BamHl human Ha-ras-1 recombinant fragment from the plasmid pEC. Thirty allelomorphs of different sizes were detected among the 172 study subjects. An association was observed between race and specific alleles. Rare alleles were more frequent in black cancer patients and trauma victims than in whites. Within each racial category, lung cancer patients had an excess of rare alleles. These data indicate the importance of controlling for racial variation when designing studies to determine human cancer risk factors. C1 NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. UNIV TURIN,CANC EPIDEMIOL UNIT,I-10124 TURIN,ITALY. TUFTS UNIV,NEW ENGLAND MED CTR,DEPT MED HEMATOL ONCOL,BOSTON,MA 02111. RP WESTON, A (reprint author), NCI,HUMAN CARCINOGENESIS LAB,BLDG 37,ROOM 2009,9600 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 29 TC 28 Z9 28 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 4 BP 265 EP 268 DI 10.1002/mc.2940040404 PG 4 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA GB107 UT WOS:A1991GB10700003 PM 1714739 ER PT J AU YA, C YI, S POIRIER, S WINTERSTEIN, D HEGAMYER, G SEED, J MALIN, S COLBURN, NH AF YA, C YI, S POIRIER, S WINTERSTEIN, D HEGAMYER, G SEED, J MALIN, S COLBURN, NH TI ISOLATION AND PARTIAL CHARACTERIZATION OF A TRANSFORMATION-ASSOCIATED SEQUENCE FROM HUMAN NASOPHARYNGEAL CARCINOMA SO MOLECULAR CARCINOGENESIS LA English DT Article DE HUMAN NASOPHARYNGEAL CARCINOMA; ONCOGENE; JB6 CELL TRANSFECTION ASSAY ID EPSTEIN-BARR-VIRUS; SQUAMOUS-CELL CARCINOMAS; NEOPLASTIC TRANSFORMATION; TUMOR PROMOTERS; EPIDERMAL-CELLS; LINES HNE-1; 3T3 CELLS; GENE; DNA; ONCOGENE AB A transforming activity associated with Chinese nasopharyngeal carcinoma (NPC) cell line CNE2 DNA has been identified by transfer into nontransformed promotion-sensitive mouse JB6(P+) C141 cells. To clone this transformation-associated sequence, we carried out three cycles of transfection, followed by cloning of anchorage-independent transformants in soft agar. A tertiary CNE/JB6 clonal transfectant cell line 625 whose DNA showed transforming activity, as indicated in both soft-agar assay and nude-mice implantation, was used to make a genomic library in the vector-lambda-dash. Using the human repeated sequence Blur 8 to screen the library, we obtained 10 human Alu-positive clones. A cloned Alu-positive insert of 16 kbp, CNE 323, was characterized in detail. CNE 323 transferred moderate transforming activity when introduced into JB6 P+ cells and showed no homology to Ha-, Ki-, or N-ras genes; human promotion sensitivity genes; src, myb, jun, myc, fos, raf, or int-2 oncogenes; or epidermal growth factor receptor. The isolated CNE 323 DNA sequence appeared to preserve the genomic structure of the original sequence found in CNE2 cells and in nude mouse tumors induced by CNE2 cells or by CNE/JB6 transfectant cells, indicating that the cloned NPC sequence was activated during NPC carcinogenesis and not during transfection or construction of the library, and that the cloned sequence or a larger sequence of which it was part played a role in tumor formation. Finally, we identified a 1.3-kb mRNA that hybridizes to a subclone of the 16-kb NPC sequence in CNE2 cell poly (A)+ RNA. C1 NCI,FREDERICK CANC RES & DEV CTR,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702. DYNCORP,PROGRAM RESOURCES INC,VIRAL CARCINOGENESIS LAB,FREDERICK,MD. DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD. NR 40 TC 2 Z9 2 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 4 BP 297 EP 307 PG 11 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA GB107 UT WOS:A1991GB10700007 ER PT J AU TODOROVIC, R DEVANESAN, PD CAVALIERI, EL ROGAN, EG PARK, SS GELBOIN, HV AF TODOROVIC, R DEVANESAN, PD CAVALIERI, EL ROGAN, EG PARK, SS GELBOIN, HV TI A MONOCLONAL-ANTIBODY TO RAT-LIVER CYTOCHROME P450 IIC11 STRONGLY AND REGIOSPECIFICALLY INHIBITS CONSTITUTIVE BENZO(A)PYRENE METABOLISM AND DNA-BINDING SO MOLECULAR CARCINOGENESIS LA English DT Article DE ENZYME INHIBITION; CARCINOGENIC METABOLISM; DNA ADDUCTS ID HEPATIC CYTOCHROME-P-450; AROMATIC-HYDROCARBONS; SEX DIFFERENCE; FEMALE RATS; MICROSOMES; BENZOPYRENE; CARCINOGENESIS; PURIFICATION; FORMS; INDUCIBILITY AB The monoclonal antibody MAb 1-68-11, prepared to constitutive cytochrome P450 IIC11 (2c/RLM5) from male Sprague-Dawley rat liver, was used to study the contribution of the class of cytochrome P450s epitopically related to P450 IIC11 to the regiospecific metabolism of benzo[a]pyrene (BP) and its binding to DNA. The effect of MAb 1-68-11 was determined on the conversion of BP to BP-9,10-dihydrodiol, BP-7,8-dihydrodiol, BP-4,5-dihydrodiol, BP phenols, and BP quinones, and on the P450-dependent DNA binding catalyzed by P450 in microsomes from uninduced male and female Wistar and Sprague-Dawley rat livers, as well as 3-methylcholanthrene- and phenobarbital (PB)-induced male Wistar rat livers. In liver microsomes from untreated male rats, MAb 1-68-11 inhibited BP-9,10-dihydrodiol formation by 80%; in liver microsomes from untreated female rats, the inhibition was 100%. BP-7,8-dihydrodiol formation was inhibited from 38 to 77% in microsomes from males and 50% in those from females. In microsomes from PB-induced rats, inhibition of the 9,10-dihydrodiol and the 7,8-dihydrodiol was 90% and 73%, respectively, whereas BP-4,5-dihydrodiol formation was enhanced 80%. In microsomes from 3-methylcholanthrene-treated rats, no inhibition of MAb 1-68-11 was observed on either the metabolism of BP or its binding to DNA. In contrast, the binding of BP to DNA was completely inhibited by MAb 1-68-11 in microsomes from uninduced male Wistar rats and 70% in PB-induced microsomes. P-32-postlabeling analysis showed that formation of the major stable adduct, BP diol epoxide bound at C-10 to the 2-amino of deoxyguanosine, was strongly inhibited in uninduced and PB-induced microsomes. Formation of the major labile BP-DNA adduct 7-(benzo[a]pyren-6-yl) guanine (BP-N7Gua) was inhibited about 60% in microsomes from untreated male Wistar rats. These results show that MAb 1-68-11 regiospecifically inhibits cytochrome P450 IIC11 and epitopically related P450s that metabolize BP at the 7,8 and 9,10 positions. MAb 1-68-11 also inhibits enzyme-catalyzed binding of BP to DNA in the specific formation of BP-N7Gua and adducts detected by the P-32-postlabeling technique. C1 UNIV NEBRASKA,EPPLEY INST RES CANC & ALLIED DIS,MED CTR,600 S 42ND ST,OMAHA,NE 68198. NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. FU NCI NIH HHS [R01-CA25176, R01-CA44686, P30-CA36727] NR 29 TC 13 Z9 14 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 4 BP 308 EP 314 DI 10.1002/mc.2940040409 PG 7 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA GB107 UT WOS:A1991GB10700008 PM 1872951 ER PT J AU BLUMBERG, PM AF BLUMBERG, PM TI COMPLEXITIES OF THE PROTEIN-KINASE-C PATHWAY SO MOLECULAR CARCINOGENESIS LA English DT Review ID SKIN TUMOR PROMOTION; PHORBOL ESTER; MOUSE SKIN; ACTIVATION; RECEPTOR; DIACYLGLYCEROL; MECHANISM; BINDING; INVIVO; BRAIN RP NCI, CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB, BETHESDA, MD 20892 USA. NR 40 TC 137 Z9 137 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0899-1987 EI 1098-2744 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 5 BP 339 EP 344 DI 10.1002/mc.2940040502 PG 6 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA GJ829 UT WOS:A1991GJ82900001 PM 1910477 ER PT J AU BATTULA, N SCHUT, HAJ THORGEIRSSON, SS AF BATTULA, N SCHUT, HAJ THORGEIRSSON, SS TI CYTOCHROME-P4501A2 CONSTITUTIVELY EXPRESSED FROM TRANSDUCED DNA MEDIATES METABOLIC-ACTIVATION AND DNA-ADDUCT FORMATION OF AROMATIC AMINE CARCINOGENS IN NIH 3T3 CELLS SO MOLECULAR CARCINOGENESIS LA English DT Article DE P4501A2 TRANSDUCTION; INSITU P4501A2 ASSAY; CARCINOGEN METABOLISM; P-32-POSTLABELING ASSAY ID MUTAGENIC ACTIVATION; STABLE EXPRESSION; MAMMALIAN-CELLS; O-DEETHYLASE; RAT-LIVER; CDNA; ARYLAMINES; INDUCTION; 2-ACETYLAMINOFLUORENE; IDENTIFICATION AB We transduced mouse cytochrome P4501A2 DNA into NIH 3T3 cells by retrovirus-mediated gene transfer. The capacity of the transduced cytochrome P4501A2 for metabolic activation and DNA-carcinogen adduct formation of aromatic amine carcinogens was investigated. Clones of NIH 3T3 cells that constitutively express cytochrome P4501A2 and controls were exposed to a prototype food-derived carcinogenic heterocyclic amine, 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), and an aromatic amine, 2-acetylaminofluorene (AAF), and their genomic DNAs were analyzed for adducts by P-32-postlabeling assays. Kinetic analysis of DNA-carcinogen adducts indicated that adduct formation was dependent on the level of the enzyme, the dose of carcinogen, and the duration of exposure. Addition of 7,8-benzoflavone, an inhibitor of P4501A2, blocked both the enzyme activity and DNA-adduct formation, indicating the specific role of P4501A2 in metabolic activation and adduct formation. Three specific IQ-DNA adducts were detected in cells expressing P4501A2. Fingerprints of the in situ DNA adducts were similar to those of the in vivo adducts in rodent hepatic DNA after the administration of IQ. A single AAF-DNA adduct was observed in cells exposed to AAF, but other minor adducts were also detected in vivo. These results show that cells expressing constitutive levels of single cytochrome P450s provide an excellent in situ model system for analyzing the catalytic specificity, metabolic activation, and genotoxicity of putative toxic, mutagenic, and carcinogenic substances. C1 NCI,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892. MED COLL OHIO,DEPT PATHOL,TOLEDO,OH 43699. FU NCI NIH HHS [R01-CA-47484] NR 41 TC 21 Z9 21 U1 2 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 5 BP 407 EP 414 DI 10.1002/mc.2940040513 PG 8 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA GJ829 UT WOS:A1991GJ82900012 PM 1910484 ER PT J AU SLAGA, TJ YUSPA, SH AF SLAGA, TJ YUSPA, SH TI SPECIAL TOPICS IN CARCINOGENESIS SO MOLECULAR CARCINOGENESIS LA English DT Editorial Material ID CHEMICALS C1 NCI,BETHESDA,MD 20892. RP SLAGA, TJ (reprint author), UNIV TEXAS,MD ANDERSON CANC CTR,SCI PK RES DIV,POB 389,SMITHVILLE,TX 78957, USA. NR 6 TC 0 Z9 0 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 6 BP 419 EP 419 DI 10.1002/mc.2940040603 PG 1 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA GX855 UT WOS:A1991GX85500002 ER PT J AU TENNANT, RW ELWELL, MR SPALDING, JW GRIESEMER, RA AF TENNANT, RW ELWELL, MR SPALDING, JW GRIESEMER, RA TI EVIDENCE THAT TOXIC INJURY IS NOT ALWAYS ASSOCIATED WITH INDUCTION OF CHEMICAL CARCINOGENESIS SO MOLECULAR CARCINOGENESIS LA English DT Article DE CHEMICAL CARCINOGENESIS; NONCARCINOGENS; CHRONIC TOXICITY; NEOPLASTIC PROCESSES; RODENT BIOASSAYS; TOXIC LESIONS ID RATS FED DIETS; CANCER; ANTIONCOGENES; CHLOROANILINE; PREDICTION; ONCOGENES; RODENTS; PROGRAM AB Long-term rodent bioassays with chemicals administered at maximum tolerated doses identify noncarcinogens as well as carcinogens. Thirty-one chemicals recently evaluated for carcinogenic potential by the National Toxicology Program provide unique data on the relationships between mutagenicity, toxicity, and carcinogenicity. Twenty-two substances were classified as carcinogens, and nine showed no evidence of carcinogenicity. Although cellular proliferation does play an intrinsic role in neoplastic processes, the responses associated with chronic toxicity in these studies were not always sufficient to induce neoplasia. Regardless of their mutagenic potential, 19 carcinogens induced toxic effects at sites that did not show neoplastic changes; similar toxic lesions were also seen among the mutagenic and nonmutagenic noncarcinogens. Although many nonmutagens induced neoplasia at sites that showed toxic effects, some of the same chemicals also exhibited toxicity at other sites that showed no neoplastic effect. These results suggest that for some chemicals, properties other than mutagenicity or toxicity may be responsible for their carcinogenic potential. RP TENNANT, RW (reprint author), NIEHS,NATL TOXICOL PROGRAM,DIV TOXICOL RES & TESTING,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 29 TC 46 Z9 46 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 6 BP 420 EP 440 DI 10.1002/mc.2940040604 PG 21 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA GX855 UT WOS:A1991GX85500003 PM 1793481 ER PT J AU SUTTER, C STRICKLAND, JE WELTY, DJ YUSPA, SH WINTER, H SCHWEIZER, J AF SUTTER, C STRICKLAND, JE WELTY, DJ YUSPA, SH WINTER, H SCHWEIZER, J TI V-HA-RAS INDUCED MOUSE SKIN PAPILLOMAS EXHIBIT ABERRANT EXPRESSION OF KERATIN K13 AS DO THEIR 7,12-DIMETHYLBENZ[A]ANTHRACENE/12-O-TETRADECANOYLPHORBOL-13-ACETATE INDUCED ANALOGS SO MOLECULAR CARCINOGENESIS LA English DT Article DE DMBA; TPA; KERATIN-13; V-HA-RAS; PAPILLOMA ID INTERNAL STRATIFIED EPITHELIA; TERMINAL DIFFERENTIATION; SQUAMOUS EPITHELIA; EPIDERMAL TUMORS; GENE-EXPRESSION; SARCOMA-VIRUSES; CARCINOGENESIS; DNA; PROGRESSION; ACTIVATION AB Introduction of the v-Ha-ras gene into primary epidermal keratinocytes, followed by grafting of these cells to animals, leads to the formation of benign epidermal tumors that resemble papillomas induced chemically by a two-stage carcinogenesis protocol. In this study, we investigated v-Ha-ras-induced papillomas for aberrant expression of type I keratin K13, previously described in 7,12-dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA)-induced mouse epidermal tumors. Papillomas produced from three independent infection series were removed 3 wk after grafting concomitant with control grafts originating from mock-, neo-, and v-fos-infected primary keratinocytes. Combined analysis of the grafts by western blotting of extracted keratins and immunofluorescence studies of frozen sections with a K13-monospecific antibody revealed K13 expression in all v-Ha-ras-induced papillomas and absence of this keratin in all control grafts. K13-positive cells in papillomas were restricted to the suprabasal cell layers of the lesions and, at this stage of papilloma development, occurred as foci of varying extensions. Analysis of genomic DNA from v-Ha-ras-induced papillomas for the methylation state of a CpG dinucleotide in the distant promoter region of the K13 gene revealed the occurrence of unmethylated DNA copies that were generated at the expense of methylated DNA copies ubiquitously present in normal epidermis. The ratio of unmethylated to methylated DNA copies correlated with the extent of suprabasal K13 protein expression. Thus, all features of aberrant K13 expression previously described in DMBA/TPA-induced papillomas were shared by v-Ha-ras-induced papillomas. C1 NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT,BETHESDA,MD 20892. RP SUTTER, C (reprint author), GERMAN CANC RES CTR,INST BIOCHEM,NEUENHEIMER FELD 280,W-6900 HEIDELBERG,GERMANY. NR 36 TC 20 Z9 20 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 6 BP 467 EP 476 DI 10.1002/mc.2940040610 PG 10 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA GX855 UT WOS:A1991GX85500009 PM 1724371 ER PT J AU GANT, TW SILVERMAN, JA BISGAARD, HC BURT, RK MARINO, PA THORGEIRSSON, SS AF GANT, TW SILVERMAN, JA BISGAARD, HC BURT, RK MARINO, PA THORGEIRSSON, SS TI REGULATION OF 2-ACETYLAMINOFLUORENE MEDIATED AND 3-METHYLCHOLANTHRENE MEDIATED INDUCTION OF MULTIDRUG RESISTANCE AND CYTOCHROME-P450IA GENE FAMILY EXPRESSION IN PRIMARY HEPATOCYTE CULTURES AND RAT-LIVER SO MOLECULAR CARCINOGENESIS LA English DT Article DE MULTIDRUG RESISTANCE; INDUCTION; 2-ACETYLAMINOFLUORENE; 3-METHYLCHOLANTHRENE; TRANSCRIPTION; CYTOCHROME ID ARYL-HYDROCARBON HYDROXYLASE; SURFACE P-GLYCOPROTEIN; MAMMALIAN-CELL LINES; MESSENGER-RNA LEVELS; YA-SUBUNIT GENE; AH RECEPTOR; MDR1 GENE; TRANSCRIPTIONAL REGULATION; INDUCIBLE EXPRESSION; RESPONSIVE ELEMENT AB Previous studies by this laboratory have indicated that expression of the multidrug resistance (mdr) gene can be increased in vivo by exposure to a variety of xenobiotics. Because of the nature of these compounds, it was proposed that mdr gene expression might, at least in part, be regulated by the arylhydrocarbon (Ah) receptor. In the present study, we used a primary hepatocyte culture model to examine the relationship between induction of cytochrome P450IA and mdr expression in vitro. Both 3-methylcholanthrene (MC) and 2-acetylaminofluorene (AAF) were efficient inducers of mdr expression in this model. Induction of mdr gene expression by both MC and AAF obeyed a log10 concentration/response relationship. In contrast, 2, 3, 7,8-tetrachlorodibenzo-P-dioxin did not induce mdr expression at concentrations that yielded maximum induction of cytochrome P450IA expression. These data suggest that mdr induction was not mediated via the Ah receptor. Nuclear run-off analysis indicated that both AAF and MC induced mdr expression by increasing transcription. Primer extension analysis indicated that mdr gene transcription was initiated at one major site 151 bp upstream of the ATG site in both the uninduced and induced state in vivo and in vitro. The sequence of the primer and the site of initiation of gene transcription indicate that the main gene induced was the mdr1b gene. C1 NCI,EXPTL CARCINOGENESIS LAB,BLDG 37,ROOM 3C28,BETHESDA,MD 20892. NR 63 TC 117 Z9 117 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PY 1991 VL 4 IS 6 BP 499 EP 509 DI 10.1002/mc.2940040614 PG 11 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA GX855 UT WOS:A1991GX85500013 PM 1686552 ER PT J AU SPORN, MB ROBERTS, AB AF SPORN, MB ROBERTS, AB TI INTERACTIONS OF RETINOIDS AND TRANSFORMING GROWTH-FACTOR-BETA IN REGULATION OF CELL-DIFFERENTIATION AND PROLIFERATION SO MOLECULAR ENDOCRINOLOGY LA English DT Review ID FACTOR-BETA-1 GENE; ACID RECEPTOR; PREVENTION; CANCER; CARCINOGENESIS; PURIFICATION; BETA; IDENTIFICATION; SUPERFAMILY; EXPRESSION RP SPORN, MB (reprint author), NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892, USA. NR 56 TC 115 Z9 117 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD JAN PY 1991 VL 5 IS 1 BP 3 EP 7 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA EW188 UT WOS:A1991EW18800001 PM 2017191 ER PT J AU HAMMONDS, RG SCHWALL, R DUDLEY, A BERKEMEIER, L LAI, C LEE, J CUNNINGHAM, N REDDI, AH WOOD, WI MASON, AJ AF HAMMONDS, RG SCHWALL, R DUDLEY, A BERKEMEIER, L LAI, C LEE, J CUNNINGHAM, N REDDI, AH WOOD, WI MASON, AJ TI BONE-INDUCING ACTIVITY OF MATURE BMP-2B PRODUCED FROM A HYBRID BMP-2A/2B PRECURSOR SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID GROWTH FACTOR-BETA; OSTEOGENIN; PROTEIN; MATRIX; DIFFERENTIATION; SEQUENCES; GENE; DNA; PURIFICATION; EXPRESSION AB The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2a has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo. C1 NIH,BONE CELL BIOL SECT,BETHESDA,MD 20892. RP HAMMONDS, RG (reprint author), GENENTECH INC,DEPT DEV BIOL,460 PT SAN BRUNO BLVD,S SAN FRANCISCO,CA 94080, USA. NR 32 TC 137 Z9 139 U1 0 U2 5 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD JAN PY 1991 VL 5 IS 1 BP 149 EP 155 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA EW188 UT WOS:A1991EW18800018 PM 2017189 ER PT J AU HAEFLIGER, JA PEITSCH, MC JENNE, DE TSCHOPP, J AF HAEFLIGER, JA PEITSCH, MC JENNE, DE TSCHOPP, J TI STRUCTURAL AND FUNCTIONAL-CHARACTERIZATION OF COMPLEMENT C8-GAMMA, A MEMBER OF THE LIPOCALIN PROTEIN FAMILY SO MOLECULAR IMMUNOLOGY LA English DT Article ID RETINOL-BINDING PROTEIN; MEMBRANE ATTACK MECHANISM; AMINO-ACID SEQUENCE; ALPHA-SUBUNIT; 8TH COMPONENT; MOLECULAR-STRUCTURE; BETA-LACTOGLOBULIN; C8; IDENTIFICATION; EXPRESSION AB Human complement component C8 exhibits an unusual structure in that it contains three chains, two of which, alpha and beta, display high sequence homology to other complement and CTL pore-forming proteins. The third chain, C8-gamma, is covalently linked to C8-alpha by a disulfide linkage; it is demonstrated that Cys40 of C8-gamma is linked to Cys164 of C8-alpha, a unique cysteine located in a loop located between the cysteine-rich LDL-receptor class A module and the membrane-inserting region of C8-alpha. C8-gamma was recently identified as a member of the lipocalin protein family, in which all proteins were either shown to, or are believed to bind small hydrophobic ligands. The present results now demonstrate that C8-gamma incorporates retinol and retinoic acid in the presence of 2 M NaCl. Molecular modeling of C8-gamma, based on the crystal structure of the homologous beta-lactoglobulin, reveals a structure of eight antiparallel beta-strands, bearing a highly hydrophobic binding pocket. The residues participating in the pocket formation are highly conserved when compared with the structures of beta-lactoglobulin and retinol-binding protein, both of which are known to interact with retinol. It is therefore proposed that C8-gamma may act as a retinol transporting protein in plasma. C1 UNIV LAUSANNE,INST BIOCHEM,CH BOVERESSES 155,CH-1066 EPALINGES,SWITZERLAND. NCI,INST MATH BIOL,FREDERICK,MD 21701. NR 40 TC 26 Z9 28 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0161-5890 J9 MOL IMMUNOL JI Mol. Immunol. PD JAN-FEB PY 1991 VL 28 IS 1-2 BP 123 EP 131 DI 10.1016/0161-5890(91)90095-2 PG 9 WC Biochemistry & Molecular Biology; Immunology SC Biochemistry & Molecular Biology; Immunology GA FD122 UT WOS:A1991FD12200016 PM 1707134 ER PT B AU MINNA, JD NAU, MM TAKAHASHI, T SCHUTTE, J CHIBA, I VIALLET, J KAYE, F WHANGPENG, J OIE, H RUSSELL, E GAZDAR, AF AF MINNA, JD NAU, MM TAKAHASHI, T SCHUTTE, J CHIBA, I VIALLET, J KAYE, F WHANGPENG, J OIE, H RUSSELL, E GAZDAR, AF BE BERGSAGEL, DE MAK, TW TI MOLECULAR PATHOGENESIS OF LUNG-CANCER SO MOLECULAR MECHANISMS AND THEIR CLINICAL APPLICATION IN MALIGNANCIES SE BRISTOL-MYERS SQUIBB CANCER SYMPOSIA LA English DT Proceedings Paper CT 12TH ANNUAL BRISTOL-MYERS SQUIBB SYMP ON CANCER RESEARCH : MOLECULAR MECHANISMS AND THEIR CLINICAL APPLICATIONS IN MALIGNANCIES CY SEP 26-27, 1989 CL TORONTO, CANADA SP ONTARIO CANC INST, PRINCESS MARGARET HOSP, BRISTOL MYERS SQUIBB RP MINNA, JD (reprint author), NCI,NAVY MED ONCOL BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 5 Z9 5 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA SAN DIEGO BN 0-12-091075-6 J9 BRIS MYER C PY 1991 VL 12 BP 63 EP 83 PG 21 WC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy GA BU44X UT WOS:A1991BU44X00004 ER PT J AU GRAFMAN, J COHEN, LG HALLETT, M AF GRAFMAN, J COHEN, LG HALLETT, M TI IS FOCAL HAND DYSTONIA ASSOCIATED WITH PSYCHOPATHOLOGY SO MOVEMENT DISORDERS LA English DT Article DE FOCAL DYSTONIA; PSYCHOPATHOLOGY AB The purpose of this study was to determine if patients with focal hand dystonia have any significant psychopathology. We studied 20 patients with hand cramps who were participating in a therapeutic trial of botulinum toxin injections. Patients were interviewed and administered the Minnesota Multiphasic Personality Inventory (MMPI), Beck Depression Inventory, Spielberger State-Trait Anxiety Scale, a finger tapping test, and a choice serial reaction time test. Behavioral ratings were also obtained. Group statistics indicated that all personality scale scores and performances on motor tasks were within normal limits. Four out of 20 patients demonstrated mild depression. Trait anxiety scores were higher than state anxiety scores, suggesting that receiving medical treatment had a beneficial effect on mood. The number of depressive symptoms endorsed on the MMPI was correlated with reaction time speed but not finger dexterity. None of the 20 patients reported a remarkable psychiatric history. These results indicate that hand cramps are not associated with serious psychopathology. RP GRAFMAN, J (reprint author), NINCDS,MED NEUROL BRANCH,COGNIT NEUROSCI SECT,BLDG 10,ROOM 5C422,BETHESDA,MD 20892, USA. OI Grafman, Jordan H./0000-0001-8645-4457 NR 0 TC 20 Z9 20 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0885-3185 J9 MOVEMENT DISORD JI Mov. Disord. PY 1991 VL 6 IS 1 BP 29 EP 35 DI 10.1002/mds.870060106 PG 7 WC Clinical Neurology SC Neurosciences & Neurology GA EP824 UT WOS:A1991EP82400004 PM 2005919 ER PT J AU HALLETT, M RAVITS, J DUBINSKY, RM GILLESPIE, MM MOINFAR, A AF HALLETT, M RAVITS, J DUBINSKY, RM GILLESPIE, MM MOINFAR, A TI A DOUBLE-BLIND TRIAL OF ISONIAZID FOR ESSENTIAL TREMOR AND OTHER ACTION TREMORS SO MOVEMENT DISORDERS LA English DT Article DE ISONIAZID; INH; ESSENTIAL TREMOR; POSTURAL ACTION TREMOR; BETA-BLOCKER; PRIMIDONE AB We conducted a double-blind trial of isoniazid in 11 patients with essential tremor and four patients with other types of postural action tremor. The tremor had not been helped by beta-blockers or primidone. Isoniazid was given in doses up to 1,200 mg daily, together with 100 mg pyridoxine, for four weeks. Results were assessed with subjective and objective scales. Only two patients with essential tremor appeared to benefit enough to continue the drug after the trial, and only one has benefited from its long-term use. Isoniazid may be useful in rare cases of essential tremor, but must be monitored carefully because of its toxicity. RP HALLETT, M (reprint author), NINCDS,MED NEUROL BRANCH,HUMAN MOTOR CONTROL SECT,BLDG 10,ROOM 5N226,BETHESDA,MD 20892, USA. NR 0 TC 8 Z9 8 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0885-3185 J9 MOVEMENT DISORD JI Mov. Disord. PY 1991 VL 6 IS 3 BP 253 EP 256 DI 10.1002/mds.870060310 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA FW226 UT WOS:A1991FW22600009 PM 1681430 ER PT J AU HALLETT, M AF HALLETT, M TI EXTENDING THE CONCEPT OF BALLISTIC MOVEMENT OVERFLOW MYOCLONUS SO MOVEMENT DISORDERS LA English DT Note RP HALLETT, M (reprint author), NINCDS,MED NEUROL BRANCH,HUMAN MOTOR CONTROL SECT,BLDG 10,ROOM 5N226,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0885-3185 J9 MOVEMENT DISORD JI Mov. Disord. PY 1991 VL 6 IS 4 BP 354 EP 354 DI 10.1002/mds.870060416 PG 1 WC Clinical Neurology SC Neurosciences & Neurology GA GN705 UT WOS:A1991GN70500014 PM 1758456 ER PT B AU JACOBSON, AE LINDERS, JTM MATTSON, MV GEORGE, C IORIO, MA AF JACOBSON, AE LINDERS, JTM MATTSON, MV GEORGE, C IORIO, MA BE KAMENKA, JM DOMINO, EF TI THE 1-(1-PHENYL-(2-METHYLCYCLOHEXYL, 3-METHYLCYCLOHEXYL, AND 4-METHYLCYCLOHEXYL)PIPERIDINES REVISITED - SYNTHESIS, STEREOCHEMISTRY, ABSOLUTE-CONFIGURATION, COMPUTER-ASSISTED MOLECULAR MODELING AND BIOLOGICAL EFFECTS SO MULTIPLE SIGMA AND PCP RECEPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION ? LA English DT Proceedings Paper CT 3RD JOINT FRENCH / UNITED-STATES SEMINAR ON MULTIPLE SIGMA AND PCP ( PHENCYCLIDINE ) RECIPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION CY SEP 15-19, 1991 CL MONTPELLIER, FRANCE SP CNRS, NATL SCI FDN, CONSEIL REG LANGUEDOC ROUSSILLON, CONSEIL GEN HERAULT, DIRECT RECH ETUDE & TECH, ECOLE NATL SUPER CHIM MONTPELLIER, UNIV MICHIGAN, AMER CYANAMID, ANAQUEST, BERLEX LABS RP JACOBSON, AE (reprint author), NIDDKD,BLDG 8,ROOM B1-22,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NPP BOOKS PI ANN ARBOR PA ANN ARBOR BN 0-916182-09-6 PY 1991 BP 61 EP 74 PG 14 WC Biochemistry & Molecular Biology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BV79L UT WOS:A1991BV79L00006 ER PT B AU ROTHMAN, RB REID, AA SILVERTHORN, M DECOSTA, BR MONN, JA THURKAUF, A JACOBSON, AE RICE, KC ROGAWSKI, MA AF ROTHMAN, RB REID, AA SILVERTHORN, M DECOSTA, BR MONN, JA THURKAUF, A JACOBSON, AE RICE, KC ROGAWSKI, MA BE KAMENKA, JM DOMINO, EF TI STRUCTURE ACTIVITY STUDIES ON THE INTERACTION OF BIOGENIC-AMINE REUPTAKE INHIBITORS AND POTASSIUM CHANNEL BLOCKERS WITH MK-801 SENSITIVE (PCP SITE-1) AND INSENSITIVE (PCP SITE-2) [H-3] TCP BINDING-SITES IN GUINEA-PIG BRAIN SO MULTIPLE SIGMA AND PCP RECEPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION ? LA English DT Proceedings Paper CT 3RD JOINT FRENCH / UNITED-STATES SEMINAR ON MULTIPLE SIGMA AND PCP ( PHENCYCLIDINE ) RECIPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION CY SEP 15-19, 1991 CL MONTPELLIER, FRANCE SP CNRS, NATL SCI FDN, CONSEIL REG LANGUEDOC ROUSSILLON, CONSEIL GEN HERAULT, DIRECT RECH ETUDE & TECH, ECOLE NATL SUPER CHIM MONTPELLIER, UNIV MICHIGAN, AMER CYANAMID, ANAQUEST, BERLEX LABS RP ROTHMAN, RB (reprint author), NIDA,ADDICT RES CTR,CLIN PSYCHOPHARMACOL,BLDG C,4940 E AVE,POB 5180,BALTIMORE,MD 21224, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NPP BOOKS PI ANN ARBOR PA ANN ARBOR BN 0-916182-09-6 PY 1991 BP 137 EP 146 PG 10 WC Biochemistry & Molecular Biology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BV79L UT WOS:A1991BV79L00011 ER PT B AU SU, TP WU, XZ CONE, EJ SHUKLA, K GUND, TM DODGE, AL PARISH, DW AF SU, TP WU, XZ CONE, EJ SHUKLA, K GUND, TM DODGE, AL PARISH, DW BE KAMENKA, JM DOMINO, EF TI CHEMICAL AND MOLECULAR MODELING CONSIDERATIONS OF PRE-084, A SELECTIVE SIGMA-LIGAND DERIVED FROM PHENCYCLIDINE SO MULTIPLE SIGMA AND PCP RECEPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION ? LA English DT Proceedings Paper CT 3RD JOINT FRENCH / UNITED-STATES SEMINAR ON MULTIPLE SIGMA AND PCP ( PHENCYCLIDINE ) RECIPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION CY SEP 15-19, 1991 CL MONTPELLIER, FRANCE SP CNRS, NATL SCI FDN, CONSEIL REG LANGUEDOC ROUSSILLON, CONSEIL GEN HERAULT, DIRECT RECH ETUDE & TECH, ECOLE NATL SUPER CHIM MONTPELLIER, UNIV MICHIGAN, AMER CYANAMID, ANAQUEST, BERLEX LABS RP SU, TP (reprint author), NIDA,ADDICT RES CTR,POB 5180,BALTIMORE,MD 21224, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NPP BOOKS PI ANN ARBOR PA ANN ARBOR BN 0-916182-09-6 PY 1991 BP 147 EP 153 PG 7 WC Biochemistry & Molecular Biology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BV79L UT WOS:A1991BV79L00012 ER PT B AU BOWEN, WD TOLENTINO, PJ HSU, KK CUTTS, JM NAIDU, SS AF BOWEN, WD TOLENTINO, PJ HSU, KK CUTTS, JM NAIDU, SS BE KAMENKA, JM DOMINO, EF TI INHIBITION OF THE CHOLINERGIC PHOSPHOINOSITIDE RESPONSE BY SIGMA-LIGANDS - DISTINGUISHING A SIGMA-RECEPTOR-MEDIATED MECHANISM FROM A MECHANISM INVOLVING DIRECT CHOLINERGIC RECEPTOR ANTAGONISM SO MULTIPLE SIGMA AND PCP RECEPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION ? LA English DT Proceedings Paper CT 3RD JOINT FRENCH / UNITED-STATES SEMINAR ON MULTIPLE SIGMA AND PCP ( PHENCYCLIDINE ) RECIPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION CY SEP 15-19, 1991 CL MONTPELLIER, FRANCE SP CNRS, NATL SCI FDN, CONSEIL REG LANGUEDOC ROUSSILLON, CONSEIL GEN HERAULT, DIRECT RECH ETUDE & TECH, ECOLE NATL SUPER CHIM MONTPELLIER, UNIV MICHIGAN, AMER CYANAMID, ANAQUEST, BERLEX LABS RP BOWEN, WD (reprint author), NIDDK,MED CHEM LAB,RECEPTOR BIOCHEM & PHARMACOL UNIT,BLDG 8,RM B1-23,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU NPP BOOKS PI ANN ARBOR PA ANN ARBOR BN 0-916182-09-6 PY 1991 BP 155 EP 167 PG 13 WC Biochemistry & Molecular Biology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BV79L UT WOS:A1991BV79L00013 ER PT B AU MCCANN, DJ SU, TP AF MCCANN, DJ SU, TP BE KAMENKA, JM DOMINO, EF TI STIMULATION OF SIGMA-LIGAND BINDING BY PHENYTOIN - APPARENT BINDING-SITE AND LIGAND SPECIFICITY SO MULTIPLE SIGMA AND PCP RECEPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION ? LA English DT Proceedings Paper CT 3RD JOINT FRENCH / UNITED-STATES SEMINAR ON MULTIPLE SIGMA AND PCP ( PHENCYCLIDINE ) RECIPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION CY SEP 15-19, 1991 CL MONTPELLIER, FRANCE SP CNRS, NATL SCI FDN, CONSEIL REG LANGUEDOC ROUSSILLON, CONSEIL GEN HERAULT, DIRECT RECH ETUDE & TECH, ECOLE NATL SUPER CHIM MONTPELLIER, UNIV MICHIGAN, AMER CYANAMID, ANAQUEST, BERLEX LABS RP MCCANN, DJ (reprint author), NIDA,ADDICT RES CTR,NEUROPHARMACOL LAB,NEUROCHEM UNIT,POB 5180,BALTIMORE,MD 21224, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NPP BOOKS PI ANN ARBOR PA ANN ARBOR BN 0-916182-09-6 PY 1991 BP 295 EP 305 PG 11 WC Biochemistry & Molecular Biology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BV79L UT WOS:A1991BV79L00026 ER PT B AU ROTHMAN, RB REID, AA KIM, A NI, Q XU, H DECOSTA, BR RICE, KC BOWEN, WD AF ROTHMAN, RB REID, AA KIM, A NI, Q XU, H DECOSTA, BR RICE, KC BOWEN, WD BE KAMENKA, JM DOMINO, EF TI THE COMPETITIVE NMDA RECEPTOR ANTAGONIST, CPP, ALLOSTERICALLY MODULATES THE NMDA RECEPTOR ASSOCIATED PHENCYCLIDINE BINDING-SITE IN THE APPARENT ABSENCE OF STERIC HINDRANCE SO MULTIPLE SIGMA AND PCP RECEPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION ? LA English DT Proceedings Paper CT 3RD JOINT FRENCH / UNITED-STATES SEMINAR ON MULTIPLE SIGMA AND PCP ( PHENCYCLIDINE ) RECIPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION CY SEP 15-19, 1991 CL MONTPELLIER, FRANCE SP CNRS, NATL SCI FDN, CONSEIL REG LANGUEDOC ROUSSILLON, CONSEIL GEN HERAULT, DIRECT RECH ETUDE & TECH, ECOLE NATL SUPER CHIM MONTPELLIER, UNIV MICHIGAN, AMER CYANAMID, ANAQUEST, BERLEX LABS RP ROTHMAN, RB (reprint author), NIDA,ADDICT RES CTR,CLIN PSYCHOPHARMACOL LAB,POB 5180,BALTIMORE,MD 21224, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NPP BOOKS PI ANN ARBOR PA ANN ARBOR BN 0-916182-09-6 PY 1991 BP 307 EP 330 PG 24 WC Biochemistry & Molecular Biology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BV79L UT WOS:A1991BV79L00027 ER PT B AU PAUL, IA ROJAS, E YOUDIM, MBH DECOSTA, BR SKOLNICK, P POLLARD, HB KUIJPERS, GAJ AF PAUL, IA ROJAS, E YOUDIM, MBH DECOSTA, BR SKOLNICK, P POLLARD, HB KUIJPERS, GAJ BE KAMENKA, JM DOMINO, EF TI SIGMA-RECEPTORS MODULATE NICOTINIC RECEPTOR FUNCTION IN ADRENAL CHROMAFFIN CELLS SO MULTIPLE SIGMA AND PCP RECEPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION ? LA English DT Proceedings Paper CT 3RD JOINT FRENCH / UNITED-STATES SEMINAR ON MULTIPLE SIGMA AND PCP ( PHENCYCLIDINE ) RECIPTOR LIGANDS : MECHANISMS FOR NEUROMODULATION AND NEUROPROTECTION CY SEP 15-19, 1991 CL MONTPELLIER, FRANCE SP CNRS, NATL SCI FDN, CONSEIL REG LANGUEDOC ROUSSILLON, CONSEIL GEN HERAULT, DIRECT RECH ETUDE & TECH, ECOLE NATL SUPER CHIM MONTPELLIER, UNIV MICHIGAN, AMER CYANAMID, ANAQUEST, BERLEX LABS RP PAUL, IA (reprint author), NIDDKD,NEUROSCI LABS,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NPP BOOKS PI ANN ARBOR PA ANN ARBOR BN 0-916182-09-6 PY 1991 BP 527 EP 541 PG 15 WC Biochemistry & Molecular Biology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BV79L UT WOS:A1991BV79L00046 ER PT J AU KRAMERS, PGN GENTILE, JM GRYSEELS, BJAM JORDAN, P KATZ, N MOTT, KE MULVIHILL, JJ SEED, JL FROHBERG, H AF KRAMERS, PGN GENTILE, JM GRYSEELS, BJAM JORDAN, P KATZ, N MOTT, KE MULVIHILL, JJ SEED, JL FROHBERG, H TI REVIEW OF THE GENOTOXICITY AND CARCINOGENICITY OF ANTISCHISTOSOMAL DRUGS - IS THERE A CASE FOR A STUDY OF MUTATION EPIDEMIOLOGY - REPORT OF A TASK GROUP ON MUTAGENIC ANTISCHISTOSOMALS SO MUTATION RESEARCH LA English DT Review DE ANTISCHISTOSOMAL DRUGS; SCHISTOSOMIASIS; HYCANTHONE; SENTINEL PHENOTYPES ID SCHISTOSOMA-HAEMATOBIUM INFECTION; SISTER-CHROMATID EXCHANGES; CULTURED MAMMALIAN-CELLS; CANCER CHEMOTHERAPEUTIC-AGENTS; ESCHERICHIA-COLI K-12; ATOMIC-BOMB SURVIVORS; MOUSE LYMPHOMA-CELLS; ORGANO-PHOSPHORUS COMPOUNDS; TYPHIMURIUM MICROSOME TEST; LINKED RECESSIVE LETHALS AB One of the interests of ICPEMC is to identify situations in which the possible induction of inherited defects in man by mutagen exposure could actually be studied. The large-scale use of mutagenic drugs in field programmes against schistosomiasis, mainly during the 1970's, was considered a possible case. An ICPEMC task group approached the problem by (1) updating the genetic toxicology data base for antischistosomal drugs, and (2) reviewing possible study areas. Expertise was combined from genetic toxicology, mutation epidemiology and tropical medicine. It was considered that: (a) if any, hycanthone would be the most appropriate candidate drug for study; (b) it would be virtually impossible to meet the basic requirements of an appropriate mutation epidemiology study, in endemic countries; (c) as more defined genetic endpoints would be selected (e.g. sentinel phenotypes) the required large sample sizes would seem prohibitive, since documentation on past programmes is limited and local demography would render the reliable tracking of substantial numbers of offspring of treated persons an almost impossible task; (d) in most endemic countries proper diagnosis and registration of inherited defects is largely lacking; (e) the problems encountered in demonstrating inherited effects in humans after heavy or chronic exposure to established animal mutagens such as ionizing radiation and cancer chemotherapy, in combination with the ambiguous nature of the animal germ cell data with hycanthone, do not particularly warrant large expectations; (f) since non-mutagenic antischistosomal drugs are now in use, the problem is academic and of low priority in the endemic countries whose medical and research resources are often limited. Thus, studying offspring of hycanthone-treated people to demonstrate the mutagenic potential of the drug in man is not a viable enterprise. (See, however, note added in proof, p. 75.) C1 NCI,BETHESDA,MD 20205. NCI,FREDERICK,MD 21701. E MERCK AG,W-6100 DARMSTADT 1,GERMANY. HOPE COLL,HOLLAND,MI 49423. LAB PARASITOL LEIDEN,LEIDEN,NETHERLANDS. CTR PESQUISAS RENE RACHOU FIOCRUZ,BR-30190 BELO HORIZONTE,BRAZIL. WHO,CH-1211 GENEVA 27,SWITZERLAND. RP KRAMERS, PGN (reprint author), NATL INST PUBL HLTH & ENVIRONM PROTECT,POB 1,3720 BA BILTHOVEN,NETHERLANDS. NR 451 TC 30 Z9 30 U1 2 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD JAN PY 1991 VL 257 IS 1 BP 49 EP 89 DI 10.1016/0165-1110(91)90019-R PG 41 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA EY632 UT WOS:A1991EY63200004 PM 1987457 ER PT J AU SINGH, NP DANNER, DB TICE, RR PEARSON, JD BRANT, LJ MORRELL, CH SCHNEIDER, EL AF SINGH, NP DANNER, DB TICE, RR PEARSON, JD BRANT, LJ MORRELL, CH SCHNEIDER, EL TI BASAL DNA DAMAGE IN INDIVIDUAL HUMAN-LYMPHOCYTES WITH AGE SO MUTATION RESEARCH LA English DT Article DE SINGLE-CELL GEL ELECTROPHORESIS; BASAL DNA DAMAGE; HUMAN LYMPHOCYTES ID AGING PROCESS; MOUSE-BRAIN; LIVER-CELLS; REPAIR; INCREASE; ABSENCE; BREAKS; TISSUE; MICE AB A role for DNA damage is central to many theories of aging, but attempts to show an increase in DNA damage with age have yielded contradictory results. However, previous experiments have been of limited sensitivity, only able to examine induced (not basal) damage or pooled (not individual) cells. In this report, we apply a novel technique (Singh et al., 1988) to directly measure basal levels of DNA single-strand breaks and alkali-labile sites in individual human peripheral blood lymphocytes (PBL) obtained from young (less than 60 years) and old (more than 60 years) male donors. This approach shows that while average changes with age are small, changes in certain individuals and in certain cells may be large: the mean increase in damage was only 12%, but the increase in a subpopulation of highly damaged lymphocytes was 5-fold. However, most of this increase was contributed by just 3 of 17 older subjects. Further characterization of these individuals may shed light on the relationship between DNA damage and aging. C1 NIA,MOLEC GENET LAB,BALTIMORE,MD 21224. INTEGRATED LAB SYST,RES TRIANGLE PK,NC 27709. NIA,BALTIMORE LONGITUDINAL STUDY AGING,BALTIMORE,MD 21224. EASTERN WASHINGTON UNIV,DEPT BIOL,CHENEY,WA 99004. RP SINGH, NP (reprint author), UNIV SO CALIF,ETHEL PERCY ANDRUS GERONTOL CTR,UNIV PK MC0191,LOS ANGELES,CA 90089, USA. NR 28 TC 95 Z9 95 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD JAN PY 1991 VL 256 IS 1 BP 1 EP 6 DI 10.1016/0921-8734(91)90026-8 PG 6 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA GP654 UT WOS:A1991GP65400001 PM 1944382 ER PT J AU MIYAKOSHI, J SCUDIERO, DA ALLALUNISTURNER, J DAY, RS AF MIYAKOSHI, J SCUDIERO, DA ALLALUNISTURNER, J DAY, RS TI THE SENSITIVITIES OF SV40-TRANSFORMED HUMAN FIBROBLASTS TO MONOFUNCTIONAL AND DNA-CROSS-LINKING ALKYLATING-AGENTS SO MUTATION RESEARCH LA English DT Article DE FIBROBLASTS, HUMAN, SV40-TRANSFORMED; DNA-CROSS-LINKING ALKYLATING AGENTS; MNNG; MMS; 1,3-BIS-(2-CHLOROETHYL)-1-NITROSUREA; 1-(2-CHLOROETHYL)-3-(2-HYDROXYETHYL)-1-NITROSOUREA; SV40-TRANSFORMED LINES ID HUMAN CELL-LINE; TRANSFORMED HUMAN-CELLS; METHYL-N-NITROSOUREA; HUMAN-TUMOR CELLS; SIMIAN-VIRUS 40; LARGE T-ANTIGEN; CROSS-LINKING; DEOXYRIBONUCLEIC-ACID; INTEGRATION SITE; MAMMALIAN-CELLS AB 4 repair-deficient (Mer-) and 2 repair-proficient (Mer+) lines of SV40-transformed human fibroblasts were assayed for colony-forming ability after treatment with MNNG, methyl methanesulfonate (MMS), 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU), and 1-(2-chloroethyl)-3-(2-hydroxyethyl)-1-nitrosourea (HECNU). The sensitivities to MMS, BCNU and HECNU of these SV40-transformed lines were similar to those of comparably treated human tumor cells observed previously. However, unlike human tumor lines, whose post-MNNG survival is strongly dependent upon Mer phenotype, SV40-transformed lines showed a lack of dependence of post-MNNG colony-forming ability on Mer phenotype. No differences in glutathione levels that might explain these differences were detected. The amounts of SV40-specific DNA and RNA among the lines were found to vary widely, but no correlation with Mer phenotype was found. C1 CROSS CANC INST,DEPT MED,MOLEC GENET & CARCINOGENESIS LAB,11560 UNIV AVE,EDMONTON T6G 1Z2,ALBERTA,CANADA. CROSS CANC INST,RADIOBIOL LAB,EDMONTON T6G 1Z2,ALBERTA,CANADA. FREDERICK CANC RES CTR,PROGRAM RESOURCES INC,FREDERICK,MD 21701. NR 51 TC 8 Z9 8 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD JAN PY 1991 VL 254 IS 1 BP 55 EP 64 DI 10.1016/0921-8777(91)90040-V PG 10 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA EP647 UT WOS:A1991EP64700006 PM 1846027 ER PT J AU SEETHARAM, S KRAEMER, KH WATERS, HL SEIDMAN, MM AF SEETHARAM, S KRAEMER, KH WATERS, HL SEIDMAN, MM TI ULTRAVIOLET MUTATIONAL SPECTRUM IN A SHUTTLE VECTOR PROPAGATED IN XERODERMA-PIGMENTOSUM LYMPHOBLASTOID-CELLS AND FIBROBLASTS SO MUTATION RESEARCH LA English DT Article DE TRANSFECTION; UV MUTAGENESIS; PLASMID; XERODERMA-PIGMENTOSUM; DNA REPAIR ID DYSPLASTIC NEVUS SYNDROME; SV40 DNA-REPLICATION; UV-INDUCED MUTATIONS; MAMMALIAN-CELLS; POINT MUTATIONS; ESCHERICHIA-COLI; LACI GENE; MUTAGENESIS; SPECIFICITY; PLASMID AB In order to examine possible cell-type specificity in mutagenic events, a shuttle-vector plasmid, pZ189, carrying a bacterial suppressor tRNA marker gene, was treated with ultraviolet radiation and propagated in Epstein-Barr virus transformed lymphoblastoid cell lines from a patient, XP12BE, with xeroderma pigmentosum (XP), group A, and a normal control. XP is a skin-cancer-prone disorder with UV hypersensitivity and defective DNA repair. Plasmid survival and mutations inactivating the marker gene were scored by transforming an indicator strain of E. coli. An earlier report on this data [Seetharam et al., (1990) J. Mol. Biol., 212, 433] indicated lower survival and higher mutation frequency with the UV-treated plasmid passed through the XP12Be(EBV) line. In the present report, sequence analysis of 198 mutant plasmids revealed a predominance of G:C --> A:T transitions with both lymphoblastoid cell lines. This finding is consistent with the bias of polymerases toward insertion of an adenine opposite non-coding photoproducts (dinucleotides or other lesions). Transversion mutagenesis, non-adjacent double mutations, and triple-base mutations may involve other mechanisms. These results were compared to similar data from a fibroblast line from the same patient [Bredberg et al., (1986) Proc. Natl. Acad. Sci. (U.S.A.), 83, 8273]. The frequency of G:C --> A:T transitions was higher, and there were fewer plasmids with multiple-base substitutions and with transversion mutations with both XP lymphoblasts and fibroblasts than with the normal lymphoblasts and fibroblasts. There were no significant differences in classes or types of mutations in the UV-treated plasmid replicated in the XP lymphoblasts and the XP fibroblasts. This suggests that the major features of UV mutagenesis in different cell types from the same individual are similar. C1 OTSUKA PHARMACEUT CO LTD,ROCKVILLE,MD 20850. RP SEETHARAM, S (reprint author), NCI,MOLEC CARCINOGENESIS LAB,BLDG 37,ROOM 3E24,BETHESDA,MD 20892, USA. FU Intramural NIH HHS [Z01 BC004517-31] NR 40 TC 32 Z9 32 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8262 J9 MUTAT RES PD JAN PY 1991 VL 254 IS 1 BP 97 EP 105 DI 10.1016/0921-8777(91)90045-Q PG 9 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA EP647 UT WOS:A1991EP64700011 PM 1986277 ER PT B AU HUFF, JE AF HUFF, JE BE CASTEGNARO, M PLESTINA, R DIRHEIMER, G CHERNOZEMSKY, IN BARTSCH, H TI CARCINOGENICITY OF OCHRATOXIN-A IN EXPERIMENTAL-ANIMALS SO MYCOTOXINS, ENDEMIC NEPHROPATHY AND URINARY TRACT TUMOURS SE IARC SCIENTIFIC PUBLICATIONS LA English DT Proceedings Paper CT INTERNATIONAL SYMP ON MYCOTOXINS, ENDEMIC NEPHROPATHY AND URINARY TRACT TUMOURS CY JUN 06-08, 1991 CL LYON, FRANCE SP INT AGCY RES CANC RP HUFF, JE (reprint author), NIEHS,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 15 Z9 15 U1 0 U2 2 PU INT AGENCY RESEARCH CANCER PI LYONS PA LYONS BN 92-832-2115-X J9 IARC SCI PUBL PY 1991 VL 115 BP 229 EP 244 PG 16 WC Oncology; Urology & Nephrology SC Oncology; Urology & Nephrology GA BW81R UT WOS:A1991BW81R00031 PM 1820337 ER PT J AU MASON, RT FALES, HM EISNER, M EISNER, T AF MASON, RT FALES, HM EISNER, M EISNER, T TI DEFENSE-MECHANISMS OF ARTHROPODS .96. WAX OF A WHITEFLY AND ITS UTILIZATION BY A CHRYSOPID LARVA SO NATURWISSENSCHAFTEN LA English DT Note ID HOMOPTERA; INSECT C1 CORNELL UNIV,NEUROBIOL & BEHAV SECT,ITHACA,NY 14853. RP MASON, RT (reprint author), NHLBI,BETHESDA,MD 20892, USA. FU NIAID NIH HHS [AI 02908] NR 15 TC 20 Z9 21 U1 0 U2 3 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0028-1042 J9 NATURWISSENSCHAFTEN JI Naturwissenschaften PD JAN PY 1991 VL 78 IS 1 BP 28 EP 30 DI 10.1007/BF01134039 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EV531 UT WOS:A1991EV53100007 PM 2023637 ER PT B AU RUSCETTI, FW JACOBSEN, SE BIRCHENALLROBERTS, M BROXMEYER, HE ENGELMANN, GL DUBOIS, C KELLER, JR AF RUSCETTI, FW JACOBSEN, SE BIRCHENALLROBERTS, M BROXMEYER, HE ENGELMANN, GL DUBOIS, C KELLER, JR BE ANAGNOSTOU, A DAINIAK, N NAJMAN, A TI ROLE OF TRANSFORMING GROWTH FACTOR-BETA-1 IN REGULATION OF HEMATOPOIESIS SO NEGATIVE REGULATORS OF HEMATOPOIESIS: STUDIES ON THEIR NATURE, ACTION, AND POTENTIAL ROLE IN CANCER THERAPY SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT 2ND INTERNATIONAL CONF ON NEGATIVE REGULATORS OF HEMATOPOIESIS CY AUG 22-25, 1990 CL PROVIDENCE, RI SP BROWN UNIV, MEM HOSP RHODE ISLAND, NHLBI, NIDDKD, SANDOZ PHARM ID COLONY-FORMING CELLS; FACTOR-BETA; BONE-MARROW; TGF-BETA; PROGENITOR CELLS; GENE-EXPRESSION; STEM-CELLS; 2 FORMS; PROLIFERATION; INHIBITION RP RUSCETTI, FW (reprint author), NCI,FREDERICK CANC RES & DEV CTR,BIOL RESPONSE MODIFIERS PROGRAM,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74102] NR 0 TC 33 Z9 34 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-676-3 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 628 BP 31 EP 43 DI 10.1111/j.1749-6632.1991.tb17220.x PG 13 WC Biochemistry & Molecular Biology; Oncology; Hematology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Hematology; Pharmacology & Pharmacy GA BT95C UT WOS:A1991BT95C00004 PM 2069310 ER PT B AU KEHRL, JH TAYLOR, A KIM, SJ FAUCI, AS AF KEHRL, JH TAYLOR, A KIM, SJ FAUCI, AS BE ANAGNOSTOU, A DAINIAK, N NAJMAN, A TI TRANSFORMING GROWTH-FACTOR-BETA IS A POTENT NEGATIVE REGULATOR OF HUMAN-LYMPHOCYTES SO NEGATIVE REGULATORS OF HEMATOPOIESIS: STUDIES ON THEIR NATURE, ACTION, AND POTENTIAL ROLE IN CANCER THERAPY SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT 2ND INTERNATIONAL CONF ON NEGATIVE REGULATORS OF HEMATOPOIESIS CY AUG 22-25, 1990 CL PROVIDENCE, RI SP BROWN UNIV, MEM HOSP RHODE ISLAND, NHLBI, NIDDKD, SANDOZ PHARM ID T-CELL LEUKEMIA; HUMAN-PLATELETS; RETINOBLASTOMA PROTEIN; SUPPRESSOR FACTOR; GENE; PURIFICATION; VIRUS; RESPONSIVENESS; EXPRESSION; INHIBITION RP KEHRL, JH (reprint author), NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892, USA. OI Kehrl, John/0000-0002-6526-159X NR 0 TC 40 Z9 41 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-676-3 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 628 BP 345 EP 353 DI 10.1111/j.1749-6632.1991.tb17267.x PG 9 WC Biochemistry & Molecular Biology; Oncology; Hematology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Hematology; Pharmacology & Pharmacy GA BT95C UT WOS:A1991BT95C00046 PM 1648884 ER PT J AU WINSKY, L JACOBOWITZ, DM AF WINSKY, L JACOBOWITZ, DM TI RADIOIMMUNOASSAY OF CALRETININ IN THE RAT-BRAIN SO NEUROCHEMISTRY INTERNATIONAL LA English DT Article ID CALCIUM-BINDING PROTEIN; NEURONS; IDENTIFICATION AB A radioimmunoassay was developed for the quantitation of the brain specific calcium binding protein, calretinin, in micropunch samples of the rat brain. The assay was sensitive, capable of detecting 2-100 ng calretinin and was specific in that no significant cross reactivity was observed with calbindin D-28k. Results indicated several brain regions with high concentrations of calretinin including the ventral cochlear nucleus (6400 ng/mg total protein), periventricular nucleus of the thalamus (4400 ng/mg), optic chiasm (3600 ng/mg), medial habenula (3400 ng/mg) and lateral lobules of the cerebellum (3100 ng/mg). Low concentrations of calretinin were found in, for example, the cerebral cortex and hippocampus. The development of this assay will be useful in future studies of experimental and physiological factors influencing calretinin content in the brain. RP WINSKY, L (reprint author), NIMH, CLIN SCI LAB, BLDG 10, ROOM 3D-48, BETHESDA, MD 20892 USA. NR 23 TC 33 Z9 33 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0197-0186 J9 NEUROCHEM INT JI Neurochem. Int. PY 1991 VL 19 IS 4 BP 517 EP 522 DI 10.1016/0197-0186(91)90070-T PG 6 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA GR786 UT WOS:A1991GR78600016 ER PT J AU GEIGER, H BAHNER, U PALKOVITS, M NICKLAS, E HUGO, C HEIDLAND, A AF GEIGER, H BAHNER, U PALKOVITS, M NICKLAS, E HUGO, C HEIDLAND, A TI EFFECTS OF ALDOSTERONE AND DEXAMETHASONE ON ATRIAL-NATRIURETIC-PEPTIDE LEVELS IN PREOPTIC AND HYPOTHALAMIC NUCLEI OF ADRENALECTOMIZED AND INTACT RATS SO NEUROENDOCRINOLOGY LA English DT Article DE ATRIAL NATRIURETIC PEPTIDE; ALDOSTERONE; DEXAMETHASONE; ADRENALECTOMY; PREOPTIC NUCLEI; HYPOTHALAMIC NUCLEI; RADIOIMMUNOASSAY ID CENTRAL NERVOUS-SYSTEM; VASOPRESSIN RELEASE; SUBFORNICAL ORGAN; BRAIN NUCLEI; POLYPEPTIDE; ATRIOPEPTIN; NEURONS; IMMUNOREACTIVITY; DEHYDRATION; INVITRO AB The effect of aldosterone and dexamethasone on the concentrations of atrial natriuretic peptide (ANP) in preoptic and hypothalamic nuclei was examined in adrenalectomized and intact rats. Five days after adrenalectomy, increased ANP levels in those brain areas which control water intake, i.e. in the subfornical organ, supraoptic nucleus, and in the so-called hypothalamic drinking centers (perifornical nucleus, lateral hypothalamic area) were measured. In contrast to this, adrenalectomy decreased ANP levels markedly in the organum vasculosum laminae terminalis and preoptic periventricular nucleus, which are reportedly involved in the central regulation of salt and water homeostasis. ANP contents of these two preoptic structures were restored almost completely by daily administration of 0.9% sodium chloride or aldosterone but not dexamethasone. The daily administration of aldosterone elevated ANP levels in the supraoptic, paraventricular and perifornical nuclei as well as in the lateral hypothalamus both in control and adrenalectomized rats. Dexamethasone which was without any significant effect on preoptic and hypothalamic nuclei in control rats elevated ANP levels in the supraoptic and perifornical nuclei and in the lateral hypothalamic area of adrenalectomized animals. Since neither adrenalectomy, nor aldosterone or dexamethasone treatment influenced plasma ANP levels, altered ANP contents measured in preoptic and hypothalamic nuclei may represent a direct effect of adrenal corticoids (mainly adlosterone) on brain ANP-containing neurons which may participate in the control of body fluid and electrolyte homeostasis. C1 SEMMELWEIS UNIV MED,SCH MED,DEPT ANAT 1,TUZOLTO 58,H-1450 BUDAPEST,HUNGARY. UNIV WURZBURG,DEPT NEPHROL,MED CLIN,W-8700 WURZBURG,GERMANY. NIMH,CELL BIOL LAB,BETHESDA,MD 20892. RI Palkovits, Miklos/F-2707-2013 NR 32 TC 20 Z9 20 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0028-3835 J9 NEUROENDOCRINOLOGY JI Neuroendocrinology PD JAN PY 1991 VL 53 IS 1 BP 63 EP 68 DI 10.1159/000125698 PG 6 WC Endocrinology & Metabolism; Neurosciences SC Endocrinology & Metabolism; Neurosciences & Neurology GA ER728 UT WOS:A1991ER72800010 PM 1828546 ER PT J AU FILLINGKATZ, MR CHOYKE, PL OLDFIELD, E CHARNAS, L PATRONAS, NJ GLENN, GM GORIN, MB MORGAN, JK LINEHAN, WM SEIZINGER, BR ZBAR, B AF FILLINGKATZ, MR CHOYKE, PL OLDFIELD, E CHARNAS, L PATRONAS, NJ GLENN, GM GORIN, MB MORGAN, JK LINEHAN, WM SEIZINGER, BR ZBAR, B TI CENTRAL-NERVOUS-SYSTEM INVOLVEMENT IN VONHIPPEL-LINDAU DISEASE SO NEUROLOGY LA English DT Article C1 NEI,CLIN SERV,BETHESDA,MD 20892. NIH,BETHESDA,MD 20892. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HARVARD UNIV,CAMBRIDGE,MA 02138. WARREN G MAGNUSSON CLIN CTR,DEPT RADIOL,BETHESDA,MD. UNIV LOUISVILLE,DEPT OPHTHALMOL,LOUISVILLE,KY 40292. UNIV LOUISVILLE,DEPT NEUROL,LOUISVILLE,KY 40292. NICHHD,HUMAN GENET BRANCH,NEUROGENET UNIT,BETHESDA,MD 20892. NINCDS,SURG NEUROL BRANCH,BETHESDA,MD 20892. NCI,SURG BRANCH,IMMUNOBIOL LAB,BETHESDA,MD 20892. NEI,CLIN BRANCH OPHTHALM GENET,BETHESDA,MD 20892. NR 8 TC 103 Z9 107 U1 0 U2 1 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0028-3878 J9 NEUROLOGY JI Neurology PD JAN PY 1991 VL 41 IS 1 BP 41 EP 46 PG 6 WC Clinical Neurology SC Neurosciences & Neurology GA ER633 UT WOS:A1991ER63300009 PM 1985294 ER PT J AU GLOWA, JR GOLD, PW AF GLOWA, JR GOLD, PW TI CORTICOTROPIN RELEASING HORMONE PRODUCES PROFOUND ANOREXIGENIC EFFECTS IN THE RHESUS-MONKEY SO NEUROPEPTIDES LA English DT Article ID BEHAVIOR; RATS; CRF AB The behavioral consequences of the central administration of corticotropin releasing hormone (CRH) in rhesus monkeys was determined using food-maintained behavior. Acute doses of CRH (0.003 ng/kg-10-mu-g/kg, i.c.v.), decreased responding for food in a dose- and time-related manner. With intermediate doses, responding occurred at a high rate until food was delivered, and then abruptly ceased for several minutes. Previous studies have attributed similar effects to the noxious properties of certain drugs. Acute doses had no effect on home cage food consumption, body weight, or responding for food on subsequent days. When CRH was given repeatedly for several days, its behavioral suppressant effects increased. Home cage food intake, body weight, and subsequent responding for food decreased for up to 6 weeks before returning to normal. These results suggest that sustained elevations in central levels of CRH can result in a sensitization to its anorexigenic effects, an effect that has not been reported in other species. Because hyperaroused clinical states such as depression and anorexia nervosa are characterized biochemically by hypercortisolism and elevated CRH in CSF, these anorexigenic effects may corroborate a potential role for CRH in affective disorders. RP GLOWA, JR (reprint author), NIMH,CLIN NEUROENDOCRINOL BRANCH,BETHESDA,MD 20892, USA. NR 28 TC 64 Z9 65 U1 0 U2 1 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH, MIDLOTHIAN, SCOTLAND EH1 3AF SN 0143-4179 J9 NEUROPEPTIDES JI Neuropeptides PD JAN PY 1991 VL 18 IS 1 BP 55 EP 61 DI 10.1016/0143-4179(91)90164-E PG 7 WC Endocrinology & Metabolism; Neurosciences SC Endocrinology & Metabolism; Neurosciences & Neurology GA EQ290 UT WOS:A1991EQ29000008 PM 2046889 ER PT J AU NESTOR, PG PARASURAMAN, R HAXBY, JV GRADY, CL AF NESTOR, PG PARASURAMAN, R HAXBY, JV GRADY, CL TI DIVIDED ATTENTION AND METABOLIC BRAIN-DYSFUNCTION IN MILD DEMENTIA OF THE ALZHEIMERS TYPE SO NEUROPSYCHOLOGIA LA English DT Article ID POSITRON EMISSION TOMOGRAPHY; SENILE DEMENTIA; GLUCOSE-UTILIZATION; WORKING MEMORY; DISEASE; ABNORMALITIES; PERFORMANCE AB The relationship between reaction time (RT) measures under single-task and dual-task conditions and resting levels of brain metabolism, as measured by positron emission tomography (PET), was examined in patients with mild dementia of the Alzheimer type (DAT) and age- and educationally-matched controls. Slowing of RT in dual-task but not single-task conditions correlated with reductions in brain metabolism in right premotor and right parietal association areas only for the mild DAT patients. The results suggest a relation between divided attention deficits and metabolic dysfunction of right frontal and parietal lobes in mild DAT patients. C1 CATHOLIC UNIV AMER,DEPT PSYCHOL,WASHINGTON,DC 20064. NIA,NEUROSCI LAB,BETHESDA,MD 20892. NR 51 TC 48 Z9 48 U1 2 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0028-3932 J9 NEUROPSYCHOLOGIA JI Neuropsychologia PY 1991 VL 29 IS 5 BP 379 EP 387 DI 10.1016/0028-3932(91)90026-5 PG 9 WC Behavioral Sciences; Neurosciences; Psychology, Experimental SC Behavioral Sciences; Neurosciences & Neurology; Psychology GA FW168 UT WOS:A1991FW16800003 PM 1886681 ER PT J AU UNGERLEIDER, LG COLBY, CL AF UNGERLEIDER, LG COLBY, CL TI SPECIAL ISSUE IN HONOR OF PRIBRAM,KARL,H. - LOCALIZATION AND DISTRIBUTION OF COGNITIVE FUNCTION - PREFACE SO NEUROPSYCHOLOGIA LA English DT Editorial Material C1 NEI,SENSORIMOTOR RES LAB,BETHESDA,MD 20892. RP UNGERLEIDER, LG (reprint author), NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0028-3932 J9 NEUROPSYCHOLOGIA JI Neuropsychologia PY 1991 VL 29 IS 6 BP 429 EP 429 DI 10.1016/0028-3932(91)90001-O PG 1 WC Behavioral Sciences; Neurosciences; Psychology, Experimental SC Behavioral Sciences; Neurosciences & Neurology; Psychology GA GB346 UT WOS:A1991GB34600001 ER PT J AU COLBY, CL DUHAMEL, JR AF COLBY, CL DUHAMEL, JR TI HETEROGENEITY OF EXTRASTRIATE VISUAL AREAS AND MULTIPLE PARIETAL AREAS IN THE MACAQUE MONKEY SO NEUROPSYCHOLOGIA LA English DT Article; Proceedings Paper CT SYMP ON BRAIN AND COGNITION : LOCALIZATION AND DISTRIBUTION OF COGNITIVE FUNCTION / 1988 MEETING OF THE SOC FOR NEUROSCIENCE CY 1988 CL TORONTO, CANADA SP SOC NEUROSCI ID SUPERIOR TEMPORAL SULCUS; RECEPTIVE-FIELD PROPERTIES; LIGHT-SENSITIVE NEURONS; STRIATE PROJECTION ZONE; PURSUIT EYE-MOVEMENTS; BROAD-BAND CHANNELS; RHESUS-MONKEY; TOPOGRAPHIC ORGANIZATION; FUNCTIONAL-PROPERTIES; VISUOTOPIC ORGANIZATION AB The definition of visual areas remains a key problem in the effort to elucidate cortical functions. Visual areas vary along a number of dimensions and are increasingly difficult to define according to traditional criteria at higher levels of the hierarchy. Three recently discovered areas in monkey parietal association cortex illustrate a new approach to this problem. Their definition depends on assessment of neuronal response properties in the alert, behaving animal combined with precise reconstruction of recording sites. This approach permits recognition of functionally distinct areas in the absence of retinotopic maps. C1 NEI,SENSORIMOTOR RES LAB,BETHESDA,MD 20892. NR 109 TC 233 Z9 233 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0028-3932 J9 NEUROPSYCHOLOGIA JI Neuropsychologia PY 1991 VL 29 IS 6 BP 517 EP 537 DI 10.1016/0028-3932(91)90008-V PG 21 WC Behavioral Sciences; Neurosciences; Psychology, Experimental SC Behavioral Sciences; Neurosciences & Neurology; Psychology GA GB346 UT WOS:A1991GB34600008 PM 1944859 ER PT J AU HAXBY, JV GRADY, CL UNGERLEIDER, LG HORWITZ, B AF HAXBY, JV GRADY, CL UNGERLEIDER, LG HORWITZ, B TI MAPPING THE FUNCTIONAL NEUROANATOMY OF THE INTACT HUMAN BRAIN WITH BRAIN WORK IMAGING SO NEUROPSYCHOLOGIA LA English DT Article; Proceedings Paper CT SYMP ON BRAIN AND COGNITION : LOCALIZATION AND DISTRIBUTION OF COGNITIVE FUNCTION / 1988 MEETING OF THE SOC FOR NEUROSCIENCE CY 1988 CL TORONTO, CANADA SP SOC NEUROSCI ID CEREBRAL BLOOD-FLOW; POSITRON-EMISSION TOMOGRAPHY; GLUCOSE METABOLIC-RATE; HUMAN VISUAL-CORTEX; OXIDATIVE-METABOLISM; CORTICAL AREAS; SELECTIVE ATTENTION; NORMAL VALUES; PET IMAGES; LOCALIZATION AB The recent development of noninvasive methods for measuring local rates of energy metabolism or blood flow in the brain has made it possible to investigate functional neuroanatomy in healthy human subjects. The best of these methods, high resolution measurement of regional cerebral blood flow (rCBF) with positron emission tomography (PET), provides a precision of anatomical localization that far exceeds that attainable with human brain lesion studies. Moreover, the study of healthy subjects avoids possible confounding effects of brain lesions, such as compensatory reorganization of brain function. PET-rCBF studies have already identified several cortical areas involved in higher-order visual processing, indicating that functional neuroimaging may yield a map of human visual cortex analogous to maps that have been developed by vision research in nonhuman primates. PET-rCBF studies of imagery and language demonstrate the potential of functional neuroimaging to map regions of human cortex that perform functions that cannot be studied so easily in nonhuman primates or perform functions that human do not share with other species. C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. RP HAXBY, JV (reprint author), NIA,NEUROSCI LAB,BLDG 10,ROOM 6C414,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 77 TC 63 Z9 63 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0028-3932 J9 NEUROPSYCHOLOGIA JI Neuropsychologia PY 1991 VL 29 IS 6 BP 539 EP 555 DI 10.1016/0028-3932(91)90009-W PG 17 WC Behavioral Sciences; Neurosciences; Psychology, Experimental SC Behavioral Sciences; Neurosciences & Neurology; Psychology GA GB346 UT WOS:A1991GB34600009 PM 1944860 ER PT J AU KOWALSKA, DM BACHEVALIER, J MISHKIN, M AF KOWALSKA, DM BACHEVALIER, J MISHKIN, M TI THE ROLE OF THE INFERIOR PREFRONTAL CONVEXITY IN PERFORMANCE OF DELAYED NONMATCHING-TO-SAMPLE SO NEUROPSYCHOLOGIA LA English DT Article; Proceedings Paper CT SYMP ON BRAIN AND COGNITION : LOCALIZATION AND DISTRIBUTION OF COGNITIVE FUNCTION / 1988 MEETING OF THE SOC FOR NEUROSCIENCE CY 1988 CL TORONTO, CANADA SP SOC NEUROSCI ID MEDIAL THALAMIC LESIONS; RHESUS-MONKEY; FRONTAL-LOBE; MEMORY; IMPAIRMENT; AMYGDALA; NUCLEUS; CORTEX; PROJECTIONS AB Findings in an earlier study (BACHEVALIER, J. and MISHKIN, M. Behav. Brain Res. 20, 249-261, 1986) indicated that ventromedial prefrontal cortex, which receives thalamic projections from the limbo-diencephalic system, is critical for visual recognition; whereas the dorsolateral prefrontal cortex, which receives no such thalamic projections, makes little or no contribution to this type of memory. In the present study, we examined the role in recognition of another prefrontal area outside the thalamic projection zone of the limbo-diencephalic system, namely, the inferior prefrontal convexity. In the first experiment, monkeys with lesions of this cortex (Group IC) were found to be impaired in relearning delayed nonmatching-to-sample (DNMS), but not on the subsequent DNMS performance test in which memory demands were greatly increased. In a second experiment, monkeys with combined lesions of the inferior and dorsolateral prefrontal cortex (Group LAT) were also found to be impaired in relearning DNMS, but in this case they were impaired, in addition, on the subsequent performance test. Neither group (IC or LAT) showed retardation in acquiring visual discrimination habits. Analysis of the DNMS behavior of both groups suggested that their deficits on this task were due not to a loss in recognition memory, but to various forms of perseverative interference. The results support the view that the inferior prefrontal convexity, like the dorsolateral prefrontal cortex, lies outside the limbo-diencephalic memory system not only anatomically but also functionally. C1 NIMH,NEUROPSYCHOL LAB,BLDG 9,ROOM 1N107,BETHESDA,MD 20892. M NENCKI INST EXPTL BIOL,DEPT NEUROPHYSIOL,PL-02093 WARSAW,POLAND. NR 33 TC 105 Z9 105 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0028-3932 J9 NEUROPSYCHOLOGIA JI Neuropsychologia PY 1991 VL 29 IS 6 BP 583 EP 600 DI 10.1016/0028-3932(91)90012-W PG 18 WC Behavioral Sciences; Neurosciences; Psychology, Experimental SC Behavioral Sciences; Neurosciences & Neurology; Psychology GA GB346 UT WOS:A1991GB34600012 PM 1944863 ER PT J AU LISTER, RG GORENSTEIN, C RISHERFLOWERS, D WEINGARTNER, HJ ECKARDT, MJ AF LISTER, RG GORENSTEIN, C RISHERFLOWERS, D WEINGARTNER, HJ ECKARDT, MJ TI DISSOCIATION OF THE ACUTE EFFECTS OF ALCOHOL ON IMPLICIT AND EXPLICIT MEMORY PROCESSES SO NEUROPSYCHOLOGIA LA English DT Article ID AMNESIA; RETRIEVAL; LORAZEPAM; RETENTION; DIAZEPAM; SYSTEMS AB The effects of alcohol (0, 0.3 and 0.6 g/kg) on learning and memory were assessed in independent groups of male student volunteers. Subjects were shown a list of words and asked to form an image of a scene involving each word 1 hr after drinking an alcohol-containing beverage. Alcohol consumption impaired the ability of subjects to explicitly remember the words in a test of free recall. However, no impairment was observed if memory for the same material was assessed implicitly using a backwards-reading or word-completion task. That is, both alcohol-and placebo-treated subjects showed similar degrees of priming. The data indicate that alcohol's effects on memory are selective. RP LISTER, RG (reprint author), NIAAA,DICBR,CLIN STUDIES LAB,BLDG 10,ROOM 3C102,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Gorenstein, Clarice/M-7075-2015 OI Gorenstein, Clarice/0000-0001-7058-9373 NR 24 TC 57 Z9 57 U1 0 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0028-3932 J9 NEUROPSYCHOLOGIA JI Neuropsychologia PY 1991 VL 29 IS 12 BP 1205 EP 1212 DI 10.1016/0028-3932(91)90034-6 PG 8 WC Behavioral Sciences; Neurosciences; Psychology, Experimental SC Behavioral Sciences; Neurosciences & Neurology; Psychology GA GW890 UT WOS:A1991GW89000006 PM 1791932 ER PT J AU KUMAR, A SCHAPIRO, MB GRADY, C HAXBY, JV WAGNER, E SALERNO, JA FRIEDLAND, RP RAPOPORT, SI AF KUMAR, A SCHAPIRO, MB GRADY, C HAXBY, JV WAGNER, E SALERNO, JA FRIEDLAND, RP RAPOPORT, SI TI HIGH-RESOLUTION PET STUDIES IN ALZHEIMERS-DISEASE SO NEUROPSYCHOPHARMACOLOGY LA English DT Article DE DEMENTIA; ALZHEIMERS DISEASE; CEREBRAL METABOLISM; PET; GLUCOSE METABOLISM ID POSITRON EMISSION TOMOGRAPHY; CEREBRAL GLUCOSE-METABOLISM; COMPUTED-TOMOGRAPHY; SENILE DEMENTIA; ATROPHY; DISTRIBUTIONS; ASYMMETRIES; PATTERNS; ANTERIOR; LAMINAR AB Forty-seven patients with probable dementia of the Alzheimer type (DAT) and 30 healthy age-matched controls were scanned using [F-18]-2-fluoro-2-deoxy-D-glucose on a Scanditronix PC 1024-7B tomograph (inplane resolution = 6 mm, axial resolution = 10 mm). Patients and controls were scanned in the resting state with their eyes patched and ears occluded. The regional cerebral metabolic rates for glucose (rCMRglc) in most major neocortical and subcortical gray matter regions, and certain metabolic ratios (rCMRglc/ calcarine rCMRglc), quantitatively discriminated even the mildly demented patients from healthy controls. The association neocortices showed metabolic abnormalities that were more severe than those in the sensorimotor and calcarine regions. All demented groups showed significant neuropsychological disturbances when compared to healthy controls. These data demonstrated widespread metabolic disturbances, particularly in the association areas, relatively early in Alzheimer's disease, and more profound involvement with disease progression. C1 NIA,NEUROSCI LAB,BLDG 10,ROOM 6C414,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. RI Friedland, Robert/A-2834-2010 OI Friedland, Robert/0000-0001-5721-1843 NR 53 TC 145 Z9 146 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0893-133X J9 NEUROPSYCHOPHARMACOL JI Neuropsychopharmacology PD JAN PY 1991 VL 4 IS 1 BP 35 EP 46 PG 12 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA ER483 UT WOS:A1991ER48300005 PM 2003867 ER PT B AU DICHIRO, G AF DICHIRO, G BE Rosa, ML TI CURRENT TRENDS IN NEURORADIOLOGY - FUNCTIONAL-STUDIES WITH MR AND PET SO NEURORADIOLOGY 1991: PROCEEDINGS OF THE 9TH NATIONAL CONGRESS OF THE ASSOCIAZIONE ITALIANA DI NEURORADIOLOGIA LA English DT Proceedings Paper CT 9th National Congress of the Associazione-Italiana-di-Neuroradiologia CY JUN 12-14, 1991 CL GENOA, ITALY SP ASSOC ITALIANA NEURORADIOL C1 NIH,DEPT NEURORADIOL,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU EDIZIONI CENTAURO PI UDINE PA VIA COSATTINI, 32, 33100 UDINE, ITALY PY 1991 BP 484 EP 484 PG 1 WC Neurosciences; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA BA06C UT WOS:A1991BA06C00098 ER PT J AU DEMIRGOREN, S MAJEWSKA, MD SPIVAK, CE LONDON, ED AF DEMIRGOREN, S MAJEWSKA, MD SPIVAK, CE LONDON, ED TI RECEPTOR-BINDING AND ELECTROPHYSIOLOGICAL EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE, AN ANTAGONIST OF THE GABA(A) RECEPTOR SO NEUROSCIENCE LA English DT Article ID PREGNENOLONE-SULFATE; RAT-BRAIN; NEURONS; BARBITURATE; COMPLEX; MODULATORS; RESPONSES; CHANNEL; LIGAND AB Recently we demonstrated that [H-3]dehydroepiandrosterone sulfate binds specifically to two populations of sites in rat brain membranes [Majewska et al. (1990) Eur. J. Pharmac. 189, 307-315]. As an extension of this work, we studied the biochemical and pharmacological properties of [H-3]dehydroepiandrosterone sulfate binding to brain membranes and the effects of dehydroepiandrosterone sulfate on GABA-induced currents in cultured neurons. [H-3]Dehydroepiandrosterone sulfate binding depended upon incubation time, pH, protein concentration, and incubation temperature. Thermal denaturation or pretreatment of the membranes with protease or phospholipase A2 reduced the binding by 54-85%. The higher affinity [H-3]dehydroepiandrosterone sulfate binding sites appeared to be associated with protein and with the GABA(A) receptor complex. Among substances known to interact with the GABA(A) receptor complex, pregnenolone sulfate, pentobarbital, and phenobarbital inhibited the binding of [H-3]dehydroepiandrosterone sulfate. High micromolar concentrations of dehydroepiandrosterone sulfate inhibited [H-3]muscimol and [H-3]flunitrazepam binding to rat brain membranes, primarily by reducing the binding affinities. Dehydroepiandrosterone sulfate also produced a concentration-dependent block of GABA-induced currents in cultured neurons from ventral mesencephalon (IC50 = 13 +/- 3-mu-M). The results of this study are consistent with an action of dehydroepiandrosterone sulfate as a negative noncompetitive modulator of the GABA(A) receptor. Because concentrations of dehydroepiandrosterone sulfate in the brain undergo physiological variations, this neurosteroid may play a vital role in regulation of neuronal excitability in the central nervous system. C1 NIDA, ADDICT RES CTR, NEUROSCI BRANCH, NEUROPHARMACOL LAB, BALTIMORE, MD 21224 USA. NR 32 TC 171 Z9 175 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0306-4522 EI 1873-7544 J9 NEUROSCIENCE JI Neuroscience PY 1991 VL 45 IS 1 BP 127 EP 135 DI 10.1016/0306-4522(91)90109-2 PG 9 WC Neurosciences SC Neurosciences & Neurology GA GK535 UT WOS:A1991GK53500012 PM 1661387 ER PT J AU LUDVIG, N BURMEISTER, V JOBE, PC KINCAID, RL AF LUDVIG, N BURMEISTER, V JOBE, PC KINCAID, RL TI ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMICAL EVIDENCE THAT THE CALMODULIN-DEPENDENT CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE IS LOCALIZED PREDOMINANTLY AT POSTSYNAPTIC SITES IN THE RAT-BRAIN SO NEUROSCIENCE LA English DT Article ID ADENYLATE-CYCLASE; PROTEIN-KINASE; GENE-EXPRESSION; NERVOUS-SYSTEM; AMP; RELEASE; CELLS; CAMP; PHOSPHORYLATION; MODULATION AB The calmodulin-dependent cyclic nucleotide phosphodiesterase represents an important junction between the Ca2+ and the cyclic AMP/cyclic GMP second messenger systems. In brain it is a major cyclic nucleotide-degrading activity and is selectively expressed in the soma and dendrites of regional output neurons [Kincaid et al. (1987) Proc. Natn. Acad. Sci. U.S.A. 84, 1118-1122]. In this study the subcellular localization of this enzyme in cerebral cortex, hippocampus and inferior colliculus of rat brain was analysed by electron microscopic immunocytochemical methods using affinity-purified antibodies. The immunoreactivity was found exclusively within neurons whereas glial cells were unstained; preabsorption of antibody with phosphodiesterase eliminated this reactivity, demonstrating the specificity of immunostaining. In the neuronal cell bodies, deposits of immunoreaction product occurred as sparse patches in the cytoplasm, and were often associated with organelles such as mitochondria, Golgi-complex and endoplasmic reticulum; nuclei, however, were free from immunoreaction product. In the neuronal processes immunoreactivity was found within dendrites and dendritic spines, whereas the myelinated axons and axon terminals were immunonegative. The postsynaptic densities of asymmetric synapses were associated with especially high concentrations of immunoreaction product. However, the immunopositive synaptic profiles appeared to be quite selective, comprising only a small percentage of the total number of synapses in the neuropil. Our results indicate that the calmodulin-dependent cyclic nucleotide phosphodiesterase is concentrated at postsynaptic sites in specific classes of neurons. This finding supports other morphological evidence indicating a primary role for cyclic nucleotide action in postsynaptic and not presynaptic structures. Furthermore, since this enzyme is regulated by Ca2+, this interface between second messenger systems seems to play a significant role in the postsynaptic integration of Ca2+-mediated neuronal inputs. C1 UNIV ILLINOIS,COLL MED,DEPT PATHOL,PEORIA,IL 61656. NIAAA,PHYSIOL PHARMACOL STUDIES LAB,IMMUNOL SECT,ROCKVILLE,MD 20852. RP LUDVIG, N (reprint author), UNIV ILLINOIS,COLL MED,DEPT BASIC SCI,PEORIA,IL 61656, USA. NR 35 TC 33 Z9 33 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PY 1991 VL 44 IS 2 BP 491 EP 500 DI 10.1016/0306-4522(91)90072-V PG 10 WC Neurosciences SC Neurosciences & Neurology GA GC929 UT WOS:A1991GC92900020 PM 1658682 ER PT J AU UNSICKER, K FLANDERS, KC CISSEL, DS LAFYATIS, R SPORN, MB AF UNSICKER, K FLANDERS, KC CISSEL, DS LAFYATIS, R SPORN, MB TI TRANSFORMING GROWTH-FACTOR-BETA ISOFORMS IN THE ADULT-RAT CENTRAL AND PERIPHERAL NERVOUS-SYSTEM SO NEUROSCIENCE LA English DT Article ID DEOXYRIBONUCLEIC-ACID CLONING; MESSENGER-RNA; RIBONUCLEIC-ACID; MAMMALIAN BRAIN; GENE-EXPRESSION; FACTOR-ALPHA; FACTOR-BETA-1; INSULIN; CELLS; NEURONS AB The distribution of transforming growth factor-beta isoforms 1, 2 and 3 and transforming growth factor-beta 2 and 3 mRNAs in adult rat central and peripheral nervous system was examined using Northern blotting and isoform specific antibodies for immunocytochemistry. Transforming growth factor-beta 2 and 3 mRNA were present in all brain areas including cerebral cortex, hippocampus, striatum, cerebellum and brainstem. In sciatic nerve, transforming growth factor-beta 3 mRNA was highly expressed, but transforming growth factor-beta 2 mRNA was not detectable. Transforming growth factor-beta 1-like immunoreactivity was confined to meninges and choroid plexus in the brain and connective tissue in peripheral ganglia and nerves. Transforming growth factor-beta 2 and 3 immunoreactivity entirely overlapped and, in general, were found in large multipolar neurons. Highest densities of immunoreactive neuronal perikarya were present in spinal cord and brainstem motor nuclei, hypothalamus, amygdaloid complex, hippocampus and cerebral cortical layers II, III and V. Most thalamic nuclei, superior colliculi, periaqueductal gray and striatum were almost devoid of transforming growth factor-beta 2- and 3-immunoreactive neurons. Fibrous astrocytes in white matter areas were intensely immunostained. Most dorsal root ganglionic neurons, their satellite cells and Schwann cells in peripheral nerves were also labeled. Transforming growth factor-beta 2- and 3-immunoreactive neurons were localized in brain regions that have been shown to contain neurons synthesizing and/or storing basic fibroblast growth factor suggesting possible opposing or synergistic effects of these peptide growth factors. However, the precise functions of local synthesis and storage of the transforming growth factor-beta isoforms in the nervous system are as yet unknown. C1 NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. RP UNSICKER, K (reprint author), UNIV MARBURG,DEPT ANAT & CELL BIOL,ROBERT KOCH STR 6,W-3550 MARBURG,GERMANY. NR 62 TC 272 Z9 275 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PY 1991 VL 44 IS 3 BP 613 EP 625 DI 10.1016/0306-4522(91)90082-Y PG 13 WC Neurosciences SC Neurosciences & Neurology GA GH271 UT WOS:A1991GH27100008 PM 1754055 ER PT J AU MERCHENTHALER, I AF MERCHENTHALER, I TI THE HYPOPHYSIOTROPIC GALANIN SYSTEM OF THE RAT-BRAIN SO NEUROSCIENCE LA English DT Article ID CENTRAL-NERVOUS-SYSTEM; HORMONE-RELEASING HORMONE; MEDIAN-EMINENCE; FENESTRATED CAPILLARIES; FLUORO-GOLD; NEURONS; IMMUNOREACTIVITY; LOCALIZATION; IDENTIFICATION; INTESTINE AB The external zone of the rat median eminence contains a large amount of galanin-immuno-reactive terminals indicating that galanin might function as a hypophysiotropic hormone. The possible sources of these galanin-containing nerve terminals were studied in the male and female rat by means of retrograde labeling in combination with fluorescence immunocytochemistry. Fluoro-Gold was used as retrograde tracer, and it was injected peripherally. Fluoro-Gold does not penetrate the blood-brain barrier, but it is taken up by nerve terminals which project to areas supplied by capillaries that lack the blood-brain barrier. The simultaneous detection of Fluoro-Gold taken up by nerve terminals in the median eminence and the endogenous galanin in thin paraffin sections has revealed that approximately 60% of the hypophysiotropic galanin cells are located in the arcuate nucleus. The remaining portion is located in the parvocellular subdivision of the paraventricular nucleus. Only scattered hypophysiotropic galanin cells are present in the medial preoptic area. RP MERCHENTHALER, I (reprint author), NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,FUNCT MORPHOL SECT,RES TRIANGLE PK,NC 27709, USA. NR 30 TC 70 Z9 70 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PY 1991 VL 44 IS 3 BP 643 EP 654 DI 10.1016/0306-4522(91)90084-2 PG 12 WC Neurosciences SC Neurosciences & Neurology GA GH271 UT WOS:A1991GH27100010 PM 1721685 ER PT J AU MERCHENTHALER, I AF MERCHENTHALER, I TI NEURONS WITH ACCESS TO THE GENERAL-CIRCULATION IN THE CENTRAL-NERVOUS-SYSTEM OF THE RAT - A RETROGRADE TRACING STUDY WITH FLUOROGOLD SO NEUROSCIENCE LA English DT Article ID CORTICOTROPIN-RELEASING-FACTOR; MEDIAN-EMINENCE; IMMUNOREACTIVE NEURONS; PARAVENTRICULAR NUCLEUS; HORSERADISH-PEROXIDASE; FACTOR CRF; BRAIN; LOCALIZATION; CELLS; IDENTIFICATION AB Central nervous system neurons which have access to the general circulation were identified by injecting the retrograde tracer Fluoro-Gold peripherally. Fluoro-Gold does not penetrate the blood-brain barrier but is taken up by nerve terminals which project to areas supplied by fenestrated capillaries or to the periphery. Fluoro-Gold-accumulating neurons were present in the following regions or cell groups of the central nervous system: diagonal band of Broca; medial preoptic area; organum vasculosum of the lamina terminalis; subfornical organ; anterior periventricular area; paraventricular nucleus; arcuate nucleus; accessory magnocellular nuclei of the hypothalamus; motor neurons of cranial nerves III-VII, and IX-XII in the brainstem and spinal cord; autonomic ganglionic cells of cranial nerve III (Westphal-Edinger nucleus) in the mesencephalon and the intermediolateral column of the spinal cord; sensory ganglia of the cranial nerve V (mesencephalic trigeminal nucleus); and the C1-C2 and A2 adrenergic cell groups in the medulla. In addition, Fluoro-Gold-accumulating neurons were seen in the sensory ganglia of cranial and spinal nerves. Retrograde labeling with Fluoro-Gold can be combined with immunocytochemistry to identify the chemical messengers within Fluoro-Gold-labeled perikarya. Although a large number of neurons are labeled in the central nervous system with Fluoro-Gold when it is administered peripherally, this technique in combination with immunocytochemistry can be a powerful tool to identify selected neuronal systems in the central nervous system. RP MERCHENTHALER, I (reprint author), NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,FUNCT MORPHOL SECT,RES TRIANGLE PK,NC 27709, USA. NR 30 TC 110 Z9 111 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PY 1991 VL 44 IS 3 BP 655 EP 662 DI 10.1016/0306-4522(91)90085-3 PG 8 WC Neurosciences SC Neurosciences & Neurology GA GH271 UT WOS:A1991GH27100011 PM 1721686 ER PT J AU INSEL, TR GELHARD, R SHAPIRO, LE AF INSEL, TR GELHARD, R SHAPIRO, LE TI THE COMPARATIVE DISTRIBUTION OF FOREBRAIN RECEPTORS FOR NEUROHYPOPHYSEAL PEPTIDES IN MONOGAMOUS AND POLYGAMOUS MICE SO NEUROSCIENCE LA English DT Article ID MATERNAL-BEHAVIOR; OXYTOCIN BINDING; FEMALE RATS; HYPOTHALAMUS; BRAIN; PROGESTERONE; INDUCTION; STEROIDS; NUCLEUS AB Several recent studies have suggested that the neurohypophyseal peptide oxytocin may have a role within the brain to mediate various forms of affiliative behavior. As the regulation of oxytocin function may be largely determined by the number and distribution of its membrane bound receptor, we investigated oxytocin receptor distribution in two Peromyscus species selected for differences in affiliative behavior. Using in vitro receptor autoradiography with the selective oxytocin receptor ligand [I-125]d(CH2)5[Tyr(Me)2,Tyr-NH2(9)]OVT ([I-125]OTA), we compared Peromyscus maniculatus, a polygamous species, to Peromyscus californicus, a monogamous species. Marked species differences in the distribution of [I-125]OTA were apparent in several brain areas, including olfactory pathways, bed nucleus of the stria terminalis, amygdala, dorsal lateral septum, and several cortical regions. In addition, gender differences in the binding pattern were evident in several regions, mostly due to sexually dimorphic patterns in the polygamous species, P. maniculatus. To further compare these species, the binding of a [H-3]arginine-vasopressin antagonist was assessed in alternate sections from those used for [I-125]OTA. Relative to oxytocin receptors, binding to arginine-vasopressin receptors showed fewer species differences, although the monogamous species appeared to have more arginine-vasopressin receptors in the neocortex and lateral septum. The striking differences in oxytocin receptor distribution are consistent with earlier studies in other rodents, suggesting that oxytocin may have an important role for mediating species-typical patterns of social affiliation. RP INSEL, TR (reprint author), NIMH,COMPARAT STUDIES BRAIN & BEHAV SECT,POOLESVILLE,MD 20837, USA. NR 33 TC 103 Z9 104 U1 1 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PY 1991 VL 43 IS 2-3 BP 623 EP 630 DI 10.1016/0306-4522(91)90321-E PG 8 WC Neurosciences SC Neurosciences & Neurology GA FY801 UT WOS:A1991FY80100029 PM 1656322 ER PT J AU PALKOVITS, M KOVACS, K MAKARA, GB AF PALKOVITS, M KOVACS, K MAKARA, GB TI CORTICOTROPIN-RELEASING HORMONE-CONTAINING NEURONS IN THE HYPOTHALAMOHYPOPHYSEAL SYSTEM IN RATS 6 WEEKS AFTER BILATERAL LESIONS OF THE PARAVENTRICULAR NUCLEUS SO NEUROSCIENCE LA English DT Article ID STALK-MEDIAN EMINENCE; IMMUNOHISTOCHEMICAL IDENTIFICATION; IMMUNOCYTOCHEMICAL LOCALIZATION; IMMUNOREACTIVE NEURONS; CRF; BRAIN; PITUITARY; SECRETION; FIBERS; OVINE AB Corticotropin-releasing factor-like immunoreactive nerve fibers and varicosities are present in the pituitary stalk and median eminence 6 weeks after bilateral lesioning of the hypothalamic paraventricular nucleus. The total immunoreactivity may reach 10% of the control density. The origin of these fibers was investigated 3 days after hypothalamic transections in paraventricular-lesioned (6 weeks postoperatively) rats. Accumulations of corticotropin-releasing factor immunostaining were observed in the proximal portions of the transected axons and in neuronal perikarya. Fibers with retrograde labeling were seen lateral and dorsolateral from the sagittally oriented knife cuts which transected the retrochiasmatic area and cells were found in the supraoptic nucleus and in the perifornical nucleus (dorsal-dorsolateral to the fornix), ipsilateral to the lesion. No corticotropin-releasing factor immunostained cells were seen in other hypothalamic or preoptic nuclei which project to the median eminence or the posterior pituitary. Corticotropin-releasing factor containing cells in the supraoptic and perifornical nuclei may have an importance in stress response in rats with long-term paraventricular lesions. C1 NIMH,CELL BIOL LAB,BETHESDA,MD 20892. HUNGARIAN ACAD SCI,INST EXPTL MED,H-1361 BUDAPEST 5,HUNGARY. RP PALKOVITS, M (reprint author), SEMMELWEIS UNIV MED,SCH MED,DEPT ANAT 1,H-1085 BUDAPEST 8,HUNGARY. RI Palkovits, Miklos/F-2707-2013; Makara, Gabor/H-8397-2013 OI Makara, Gabor/0000-0001-9220-0373 NR 28 TC 21 Z9 21 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PY 1991 VL 42 IS 3 BP 841 EP 851 DI 10.1016/0306-4522(91)90048-S PG 11 WC Neurosciences SC Neurosciences & Neurology GA FT330 UT WOS:A1991FT33000018 PM 1956519 ER PT J AU ROSARIO, LM STUTZIN, A CRAGOE, EJ POLLARD, HB AF ROSARIO, LM STUTZIN, A CRAGOE, EJ POLLARD, HB TI MODULATION OF INTRACELLULAR PH BY SECRETAGOGUES AND THE NA+/H+ ANTIPORTER IN CULTURED BOVINE CHROMAFFIN CELLS SO NEUROSCIENCE LA English DT Article ID SMOOTH-MUSCLE CELLS; CYTOPLASMIC PH; H+; MEMBRANE; EXCHANGE; ACIDIFICATION; EXOCYTOSIS; ACTIVATION; PLATELETS; SECRETION AB The possible physiological role of cytosolic pH changes in adrenal medullary chromaffin cell secretion was examined by investigating the effects of catecholamine secretagogues on cytosolic pH, which was monitored using the intracellular fluorescent indicator 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Anti-fluorescein antibodies were used to reduce background fluorescence from extracellular 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Stimulation with both cholinergic agonists (acetylcholine, nicotine) and a depolarizing agent (high K+) transiently acidified the cytosol of the chromaffin cell. This acidification was antagonized by reducing extracellular Ca2+ concentration and by Ca2+ antagonists (Co2+, verapamil), indicating that it occurred secondarily to Ca2+ influx, possibly as a result of exchange of Ca2+ ions for protons across organelle membranes. Taken together with previously published data [Kuijpers G. A. J. et al. (1989) J. biol. Chem. 264, 698-705] showing no effect of cytosolic acidification on nicotine-induced catecholamine secretion, these results indicate that secretagogue-induced cytosolic pH changes do not represent a causal step in stimulus-secretion coupling of the chromaffin cell. The cytosolic pH recovery to pre-stimulatory cytosolic pH levels was delayed by amiloride and by 5-(N,N-dimethyl)amiloride, at concentrations that otherwise substantially inhibited cytosolic pH recovery from the rebound acidification induced by a 40-fold sudden dilution of NH4Cl. This latter type of recovery results from activation of an Na+/H+ exchange mechanism. Therefore, the data suggest that Na+/H+ exchange is actively involved in the dissipation of the small acid loads generated by secretagogues. C1 NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD. UNIV CHILE,SCH MED,DEPT EXPTL MED,SANTIAGO,CHILE. NR 27 TC 15 Z9 15 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PY 1991 VL 41 IS 1 BP 269 EP 276 DI 10.1016/0306-4522(91)90215-A PG 8 WC Neurosciences SC Neurosciences & Neurology GA FH564 UT WOS:A1991FH56400020 PM 1647503 ER PT J AU HERKENHAM, M LITTLE, MD BANKIEWICZ, K YANG, SC MARKEY, SP JOHANNESSEN, JN AF HERKENHAM, M LITTLE, MD BANKIEWICZ, K YANG, SC MARKEY, SP JOHANNESSEN, JN TI SELECTIVE RETENTION OF MPP+ WITHIN THE MONOAMINERGIC SYSTEMS OF THE PRIMATE BRAIN FOLLOWING MPTP ADMINISTRATION - AN INVIVO AUTORADIOGRAPHIC STUDY SO NEUROSCIENCE LA English DT Article ID VENTRAL TEGMENTAL AREA; SUBSTANTIA NIGRA; 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE MPTP; CATECHOLAMINERGIC NEURONS; DOPAMINE NEURONS; NEUROTOXIN MPTP; MONKEY; PARKINSONISM; METABOLITE; N-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE AB 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) selectively destroys dopaminergic neurons of the substantia nigra pars compacta in humans and other primates, producing a parkinsonian condition. MPTP is metabolized to the toxin 1-methyl-4-phenylpyridine (MPP+) which is taken up by dopamine terminals. The subsequent events culminating in cell death in the substantia nigra pars compacta are not understood. To examine these events we first produced a chronic hemiparkinsonian condition in monkeys by administering a toxic dose of MPTP via the right carotid artery. One year later, these monkeys were given a trace dose of [C-14]MPTP intravenously and allowed to survive 1,3, or 10 days. In two acute conditions, monkeys were either given the radiolabeled trace dose intravenously immediately following the toxic intracarotid dose, or were given a single toxic intracarotid radiolabeled dose, and allowed to survive 1, 3, or 10 days. We show by histology and autoradiography that the chronic hemiparkinsonian condition is characterized by selective unilateral loss of nigrostriatal dopamine neurons and absence of MPP+ retention in the caudate-putamen. In the acute conditions, MPP+ is accumulated and selectively retained in high concentrations in the caudate-putamen bilaterally and throughout the nigrostriatal pathway only on the side receiving the toxic dose. In the substantia nigra pars compacta, MPP+ is accumulated in very low concentrations in the dopamine cell bodies and is not selectively retained there. At 10 days survival, the caudate-putamen on the side receiving the toxic dose loses its ability to retain MPP+. The apparent degeneration of the dopamine axon terminals in the caudate-putamen and the development of Parkinson-like behavioral signs seen at 10 days survival were observed to precede the loss of cell bodies in the substantia nigra, which appeared normal by the criteria of Nissl staining and neuromelanin content at all time points in the acute conditions. Other areas of dense MPP+ retention in all cases include noradrenergic and serotonergic cell groups and noradrenergic pathways. MPP+ in the locus coeruleus and other caudal catecholaminergic cell groups is apparently retrogradely transported there after uptake in terminal regions, and although it is retained in high concentrations, no cell loss occurs. These findings suggest that experimentally induced Parkinsonism results from molecular events initiated in the neostriatum and selectively elaborated in the nigrostriatal pathway, ultimately resulting in the death of substantia nigra pars compacta dopamine neurons. They do not support a significant role for neuromelanin binding in the toxicity of MPP+. C1 NIMH,FUNCT NEUROANAT SECT,BLDG 36,RM 2D-15,BETHESDA,MD 20892. NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NINCDS,SURG NEUROL BRANCH,BETHESDA,MD 20892. OI Herkenham, Miles/0000-0003-2228-4238 NR 35 TC 148 Z9 150 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PY 1991 VL 40 IS 1 BP 133 EP 158 DI 10.1016/0306-4522(91)90180-V PG 26 WC Neurosciences SC Neurosciences & Neurology GA EY924 UT WOS:A1991EY92400012 PM 2052148 ER PT J AU TILSON, HA HONG, JS SOBOTKA, TJ AF TILSON, HA HONG, JS SOBOTKA, TJ TI HIGH-DOSES OF ASPARTAME HAVE NO EFFECTS ON SENSORIMOTOR FUNCTION OR LEARNING AND MEMORY IN RATS SO NEUROTOXICOLOGY AND TERATOLOGY LA English DT Article DE ASPARTAME; NEUROBEHAVIORAL TOXICITY; SENSORIMOTOR FUNCTION; LEARNING AND MEMORY ID CENTRAL NERVOUS-SYSTEM; ACOUSTIC STARTLE; INDUCED SEIZURES; COLCHICINE; HIPPOCAMPUS; MICE; SUSCEPTIBILITY; NEUROTOXICITY; STRYCHNINE; CHEMICALS AB Acute or repeated (14 days) intragastric administration of L-d-aspartyl-L-phenylalanine methyl ester (aspartame) suspended in saline and Tween-80 in doses of up to 1,000 mg/kg had no significant effect in male Fischer-344 rats on routine measures of sensorimotor function, including spontaneous motor activity, acoustic startle reflex and prepulse inhibition. Other experiments found that aspartame (500 or 1,000 mg/kg) had no significant effect on acquisition of passive or active avoidance or a spatial, reference memory task in the Morris water maze. A series of separate studies found that aspartame had no effects in rats fasted 24 hours prior to testing, or if it was suspended in carboxymethylcellulose or administered by the intraperitoneal route. Under the conditions of these experiments, large doses of aspartame have no significant neurobiological effects in adult rats as measured by procedures known to be sensitive to the neurobiological effects of neurotoxicants, including convulsants, organochlorine insecticides and heavy metals. C1 NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,RES TRIANGLE PK,NC 27709. US FDA,CFSAN,NEUROBEHAV TOXICOL TEAM,WASHINGTON,DC 20204. RP TILSON, HA (reprint author), US EPA,DIV NEUROTOXICOL,MD-74B,RES TRIANGLE PK,NC 27711, USA. NR 50 TC 8 Z9 8 U1 2 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0892-0362 J9 NEUROTOXICOL TERATOL JI Neurotoxicol. Teratol. PD JAN-FEB PY 1991 VL 13 IS 1 BP 27 EP 35 DI 10.1016/0892-0362(91)90024-Q PG 9 WC Neurosciences; Toxicology SC Neurosciences & Neurology; Toxicology GA EU560 UT WOS:A1991EU56000005 PM 2046625 ER PT J AU OLDS, JL ALKON, DL AF OLDS, JL ALKON, DL TI A ROLE FOR PROTEIN-KINASE-C IN ASSOCIATIVE LEARNING SO NEW BIOLOGIST LA English DT Review DE LEARNING AND MEMORY; PROTEIN KINASE-C; ASSOCIATIONS; HIPPOCAMPUS; 2ND MESSENGER SYSTEMS; RAT; RABBIT; NICTITATING MEMBRANE; HERMISSENDA-CRASSICORNIS ID LONG-TERM POTENTIATION; RAT-BRAIN; IMMUNOCYTOCHEMICAL LOCALIZATION; CONDITIONED MODIFICATION; MEMBRANE CURRENTS; AUTORADIOGRAPHIC ANALYSIS; PHOTOTACTIC BEHAVIOR; ALZHEIMERS-DISEASE; PYRAMIDAL NEURONS; K+ CURRENTS C1 NIH,MOLEC & CELLULAR NEUROBIOL LAB,BETHESDA,MD 20892. RI Olds, James/D-2867-2011 NR 81 TC 43 Z9 43 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD JAN PY 1991 VL 3 IS 1 BP 27 EP 35 PG 9 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK716 UT WOS:A1991GK71600004 PM 2039766 ER PT J AU SCHINDLER, JM AF SCHINDLER, JM TI ZEBRAFISH - DROSOPHILA WITH A SPINE (BUT CAN THEY FLY) SO NEW BIOLOGIST LA English DT Article C1 NICHHD,GENET & TERATOL BRANCH,BETHESDA,MD 20892. NR 0 TC 4 Z9 4 U1 0 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD JAN PY 1991 VL 3 IS 1 BP 47 EP 49 PG 3 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK716 UT WOS:A1991GK71600007 PM 1674886 ER PT J AU YARMOLINSKY, MB AF YARMOLINSKY, MB TI LE CELL-CYCLE SO NEW BIOLOGIST LA English DT Article C1 NCI,BIOCHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD JAN PY 1991 VL 3 IS 1 BP 50 EP 56 PG 7 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK716 UT WOS:A1991GK71600008 PM 1674887 ER PT J AU LISZIEWICZ, J RAPPAPORT, J DHAR, R AF LISZIEWICZ, J RAPPAPORT, J DHAR, R TI TAT-REGULATED PRODUCTION OF MULTIMERIZED TAR RNA INHIBITS HIV-1 GENE-EXPRESSION SO NEW BIOLOGIST LA English DT Article DE HIV-1; TAT; TAR; GENE THERAPY ID HUMAN-IMMUNODEFICIENCY-VIRUS; LONG TERMINAL REPEAT; TRANS-ACTIVATOR GENE; HTLV-III; HUMAN CHROMOSOME-12; RESPONSIVE REGION; NUCLEAR-PROTEIN; MESSENGER-RNA; NASCENT RNA; CELLS C1 NCI,MOLEC VIROL LAB,BETHESDA,MD 20892. UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093. NR 41 TC 65 Z9 65 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD JAN PY 1991 VL 3 IS 1 BP 82 EP 89 PG 8 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK716 UT WOS:A1991GK71600012 PM 2039768 ER PT J AU THIERRY, F HOWLEY, PM AF THIERRY, F HOWLEY, PM TI FUNCTIONAL-ANALYSIS OF E2-MEDIATED REPRESSION OF THE HPV18 P105 PROMOTER SO NEW BIOLOGIST LA English DT Article DE HPV; TRANSCRIPTIONAL REGULATION; REPRESSION; DNA-PROTEIN BINDING ID BOVINE PAPILLOMAVIRUS TYPE-1; E2 GENE-PRODUCT; CERVICAL-CARCINOMA CELLS; DNA RECOGNITION SEQUENCE; CARBOXY-TERMINAL DOMAIN; REGULATORY REGION; MUTATIONAL ANALYSIS; BINDING-PROTEIN; ENHANCER; TRANSCRIPTION C1 NCI,TUMOR VIRUS BIOL LAB,BETHESDA,MD 20892. NR 47 TC 117 Z9 119 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1043-4674 J9 NEW BIOL PD JAN PY 1991 VL 3 IS 1 BP 90 EP 100 PG 11 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA GK716 UT WOS:A1991GK71600013 PM 1645591 ER PT B AU MCLACHLAN, JA NELSON, KG TAKAHASHI, T BOSSERT, NL NEWBOLD, RR KORACH, KS AF MCLACHLAN, JA NELSON, KG TAKAHASHI, T BOSSERT, NL NEWBOLD, RR KORACH, KS BE HOCHBERG, RB NAFTOLIN, F TI DO GROWTH-FACTORS MEDIATE ESTROGEN ACTION IN THE UTERUS SO NEW BIOLOGY OF STEROID HORMONES SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT SYMP ON THE NEW BIOLOGY OF STEROID HORMONES CY SEP 03-04, 1990 CL BUDAPEST, HUNGARY SP SERONO FDN RP MCLACHLAN, JA (reprint author), NIEHS,REPROD & DEV TOXICOL LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-653-5 J9 SERONO SYM PY 1991 VL 74 BP 337 EP 344 PG 8 WC Biochemistry & Molecular Biology; Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Endocrinology & Metabolism GA BU61H UT WOS:A1991BU61H00026 ER PT B AU GONZALEZ, FJ GELBOIN, HV AF GONZALEZ, FJ GELBOIN, HV BE GLEDHILL, BL MAURO, F TI HUMAN CYTOCHROMES-P450 - EVOLUTION, CATALYTIC ACTIVITIES AND INTERINDIVIDUAL VARIATIONS IN EXPRESSION SO NEW HORIZONS IN BIOLOGICAL DOSIMETRY SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT INTERNATIONAL SYMP ON TRENDS IN BIOLOGICAL DOSIMETRY CY OCT 23-27, 1990 CL LERICI, ITALY SP ENTE NAZL ENERGIA ATOM, US DOE, COMMISS EUROPEAN COMMUNITY, US EPA RP GONZALEZ, FJ (reprint author), NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. NR 0 TC 24 Z9 24 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 372 BP 11 EP 20 PG 10 GA BU89Y UT WOS:A1991BU89Y00002 PM 1956910 ER PT B AU POIRIER, MC WESTON, A AF POIRIER, MC WESTON, A BE GLEDHILL, BL MAURO, F TI DNA ADDUCT DETERMINATION IN HUMANS SO NEW HORIZONS IN BIOLOGICAL DOSIMETRY SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT INTERNATIONAL SYMP ON TRENDS IN BIOLOGICAL DOSIMETRY CY OCT 23-27, 1990 CL LERICI, ITALY SP ENTE NAZL ENERGIA ATOM, US DOE, COMMISS EUROPEAN COMMUNITY, US EPA RP POIRIER, MC (reprint author), NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892, USA. NR 0 TC 15 Z9 15 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 372 BP 205 EP 218 PG 14 GA BU89Y UT WOS:A1991BU89Y00020 PM 1956919 ER PT B AU HARRIS, CC AF HARRIS, CC BE GLEDHILL, BL MAURO, F TI MOLECULAR EPIDEMIOLOGY OF HUMAN CANCER IN THE 1990S SO NEW HORIZONS IN BIOLOGICAL DOSIMETRY SE PROGRESS IN CLINICAL AND BIOLOGICAL RESEARCH LA English DT Proceedings Paper CT INTERNATIONAL SYMP ON TRENDS IN BIOLOGICAL DOSIMETRY CY OCT 23-27, 1990 CL LERICI, ITALY SP ENTE NAZL ENERGIA ATOM, US DOE, COMMISS EUROPEAN COMMUNITY, US EPA RP HARRIS, CC (reprint author), NCI,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK J9 PROG CLIN BIOL RES JI Prog.Clin.Biol.Res. PY 1991 VL 372 BP 543 EP 558 PG 16 GA BU89Y UT WOS:A1991BU89Y00049 PM 1956947 ER PT B AU OBRIEN, SJ BENVENISTE, RE NASH, WG MARTENSON, JS EICHELBERGER, MA WILDT, DE BUSH, M WAYNE, RK GOLDMAN, D AF OBRIEN, SJ BENVENISTE, RE NASH, WG MARTENSON, JS EICHELBERGER, MA WILDT, DE BUSH, M WAYNE, RK GOLDMAN, D BE WARREN, L KOPROWSKI, H TI MOLECULAR-BIOLOGY AND EVOLUTIONARY-THEORY - THE GIANT PANDAS CLOSEST RELATIVES SO NEW PERSPECTIVES ON EVOLUTION: PROCEEDINGS OF A MULTIDISCIPLINARY SYMPOSIUM DESIGNED TO INTERRELATE RECENT DISCOVERIES AND NEW INSIGHTS IN THE FIELD OF EVOLUTION SE WISTAR SYMPOSIUM SERIES LA English DT Proceedings Paper CT SYMP ON NEW PERSPECTIVES ON EVOLUTION CY APR 18-19, 1990 CL UNIV PENN, WISTAR INST, PHILADELPHIA, PA SP WISTAR INST, ALFRED P SLOAN FDN, DOLPHINGER MCMAHON FDN, WILEY LISS HO UNIV PENN, WISTAR INST RP OBRIEN, SJ (reprint author), NCI,FREDERICK CANC RES FACIL,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21701, USA. NR 0 TC 2 Z9 4 U1 1 U2 1 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56068-5 J9 WIST SYMP S PY 1991 VL 4 BP 225 EP 250 PG 26 WC Biology SC Life Sciences & Biomedicine - Other Topics GA BT24B UT WOS:A1991BT24B00015 ER PT B AU SACHS, DH SHARABI, Y SYKES, M AF SACHS, DH SHARABI, Y SYKES, M BE CHAMPLIN, RE GALE, RP TI CHIMERISM AND THE INDUCTION OF TRANSPLANTATION TOLERANCE SO NEW STRATEGIES OF BONE MARROW TRANSPLANTATION SE UCLA SYMPOSIA ON MOLECULAR AND CELLULAR BIOLOGY - NEW SERIES LA English DT Proceedings Paper CT 5TH SYMP ON NEW STRATEGIES IN BONE MARROW TRANSPLANTATION CY JAN 20-27, 1990 CL KEYSTONE, CO SP SANDOZ PHARM, UNIV CALIF LOS ANGELES RP SACHS, DH (reprint author), NCI,IMMUNOL BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU WILEY-LISS, INC PI NEW YORK PA NEW YORK BN 0-471-56065-0 J9 UCLA SYM BI PY 1991 VL 137 BP 21 EP 28 PG 8 WC Oncology; Immunology; Medicine, Research & Experimental; Pathology; Surgery SC Oncology; Immunology; Research & Experimental Medicine; Pathology; Surgery GA BS26F UT WOS:A1991BS26F00004 ER PT B AU LONDON, ED DAWSON, VL MANTIONE, CR AF LONDON, ED DAWSON, VL MANTIONE, CR BE KAMEYAMA, T NABESHIMA, T DOMINO, EF TI SPECIFIC BINDING-SITES FOR POLYAMINES IN MAMMALIAN BRAIN SO NMDA RECEPTOR RELATED AGENTS : BIOCHEMISTRY, PHARMACOLOGY AND BEHAVIOR LA English DT Proceedings Paper CT SATELLITE SYMP ON NMDA ( N-METHYL-D-ASPARTATE ) RECEPTOR RELATED AGENTS AT THE 17TH MEETING OF THE COLLEGIUM-INTERNATIONALE-NEUROPSYCHOPHARMACOLOGICUM CY SEP 15-17, 1990 CL NAGOYA, JAPAN SP COLLEGIUM INT NEUROPSYCHOPHARMACOLOGICUM, ARAX, BANYU PHARM, DIANIPPON PHARM, FUJIREBIO, HILACHI CHEM, HOECHST JAPAN, KYOWA HAKKO KOGYO, MARUKO PHARM, MARUZEN PHARM RP LONDON, ED (reprint author), NIDA,ADDICT RES CTR,NEUROSCI BRANCH,NEUROPHARMACOL LAB,POB 5180,BALTIMORE,MD 21224, USA. NR 0 TC 8 Z9 8 U1 0 U2 0 PU NPP BOOKS PI ANN ARBOR PA ANN ARBOR BN 0-916182-08-8 PY 1991 BP 71 EP 78 PG 8 WC Biochemistry & Molecular Biology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BV79N UT WOS:A1991BV79N00007 ER PT B AU SU, TP WU, XZ SPIVAK, CE LONDON, ED BELL, JA AF SU, TP WU, XZ SPIVAK, CE LONDON, ED BELL, JA BE KAMEYAMA, T NABESHIMA, T DOMINO, EF TI BINDING-SITES ON INTACT NCB-20 CELLS SUGGEST SIGMA RECEPTOR MULTIPLICITY - SIGMA1 AND SIGMA2 SO NMDA RECEPTOR RELATED AGENTS : BIOCHEMISTRY, PHARMACOLOGY AND BEHAVIOR LA English DT Proceedings Paper CT SATELLITE SYMP ON NMDA ( N-METHYL-D-ASPARTATE ) RECEPTOR RELATED AGENTS AT THE 17TH MEETING OF THE COLLEGIUM-INTERNATIONALE-NEUROPSYCHOPHARMACOLOGICUM CY SEP 15-17, 1990 CL NAGOYA, JAPAN SP COLLEGIUM INT NEUROPSYCHOPHARMACOLOGICUM, ARAX, BANYU PHARM, DIANIPPON PHARM, FUJIREBIO, HILACHI CHEM, HOECHST JAPAN, KYOWA HAKKO KOGYO, MARUKO PHARM, MARUZEN PHARM RP SU, TP (reprint author), NIDA,ADDICT RES CTR,NEUROPHARMACOL LAB,POB 5180,BALTIMORE,MD 21224, USA. NR 0 TC 8 Z9 8 U1 0 U2 0 PU NPP BOOKS PI ANN ARBOR PA ANN ARBOR BN 0-916182-08-8 PY 1991 BP 227 EP 233 PG 7 WC Biochemistry & Molecular Biology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BV79N UT WOS:A1991BV79N00022 ER PT J AU DUNN, BB CHANNING, MA ADAMS, HR GOLDSTEIN, DS KIRK, KL KIESEWETTER, DO AF DUNN, BB CHANNING, MA ADAMS, HR GOLDSTEIN, DS KIRK, KL KIESEWETTER, DO TI A SINGLE COLUMN, RAPID QUALITY-CONTROL PROCEDURE FOR 6-[F-18]FLUORO-L-DOPA AND 6-[F-18]FLUORODOPAMINE PET IMAGING AGENTS SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article ID COMPLETE HEPATECTOMY; DOPAMINE; L-3,4-DIHYDROXYPHENYLALANINE; NEUROTRANSMITTERS; METABOLITES; TISSUES; PLASMA; RATS AB 6-[F-18]Fluoro-L-dopa and 6-[F-18]fluorodopamine are promising PET imaging agents for visualizing cerebral dopaminergic centers and cardiac sympathetic innervation and function. Administration to humans requires a means to determine the purity before injection. We describe such a method using HPLC with u.v. and radioactivity detection and a single high-speed C-18 column with gradient elution. The procedure can resolve within 10 min these fluorinated catechols, their isomers, and dihydroxyphenylalanine. The chemical and radiochemical purity, and specific activity, can be determined before injection. C1 NHLBI,HYPERTENS ENDOCRINE BRANCH,BETHESDA,MD 20892. NIDDKD,CHEM LAB,BETHESDA,MD 20892. RP DUNN, BB (reprint author), NIH,WARREN G MAGNUSON CLIN CTR,DEPT NUCL MED,CYCLOTRON RADIOCHEM SECT,BETHESDA,MD 20892, USA. NR 19 TC 8 Z9 8 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 2 BP 209 EP 213 PG 5 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EY738 UT WOS:A1991EY73800009 ER PT J AU WASHBURN, LC SUN, TTH LEE, YCC BYRD, BL HOLLOWAY, EC CROOK, JE STUBBS, JB STABIN, MG BRECHBIEL, MW GANSOW, OA STEPLEWSKI, Z AF WASHBURN, LC SUN, TTH LEE, YCC BYRD, BL HOLLOWAY, EC CROOK, JE STUBBS, JB STABIN, MG BRECHBIEL, MW GANSOW, OA STEPLEWSKI, Z TI COMPARISON OF 5 BIFUNCTIONAL CHELATE TECHNIQUES FOR Y-90 LABELED MONOCLONAL-ANTIBODY CO17-1A SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article ID Y-90; RADIOIMMUNOTHERAPY; CARCINOMA; ORGAN; AGENT; MICE; DTPA AB Monoclonal antibody CO17-1A was radiolabeled with Y-90 by five bifunctional chelate techniques. Radiation absorbed dose estimates for normal organs and tumor were calculated for each preparation based on timed tissue distribution studies in nude mice bearing SW 948 human colorectal carcinoma xenografts. The cyclic DTPA anhydride technique was inferior to the four other techniques studied. Data for SCN-Bz-DTPA and SCN-Bz-Mx-DTPA, which were conjugated to epsilon-lysyl amino groups, were similar to those for NH2-Bz-DTPA and NH2-Bz-Mx-DTPA, which were conjugated site specifically to oligo-saccharides. C1 NCI,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. WISTAR INST,PHILADELPHIA,PA 19104. RP WASHBURN, LC (reprint author), OAK RIDGE ASSOCIATED UNIV,DIV MED SCI,POB 117,OAK RIDGE,TN 37831, USA. NR 21 TC 29 Z9 29 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 3 BP 313 EP 321 PG 9 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA FF205 UT WOS:A1991FF20500007 ER PT J AU GANSOW, OA AF GANSOW, OA TI NEWER APPROACHES TO THE RADIOLABELING OF MONOCLONAL-ANTIBODIES BY USE OF METAL-CHELATES SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2ND INTERNATIONAL CONF ON DIAGNOSIS AND THERAPY WITH MONOCLONAL ANTIBODIES CY APR 26-28, 1990 CL NAPLES, ITALY ID COLON CARCINOMA XENOGRAFTS; COVALENT ATTACHMENT; DTPA; INVIVO; RADIOIMMUNOTHERAPY; Y-90; DERIVATIVES; CONJUGATION; GENERATOR; ALBUMIN AB Monoclonal antibodies (mAbs) radiolabeled by use of metal chelators are being investigated in the laboratory for use in clinical trials. In-111 is presently employed for diagnostic scintigraphy, but its applications are limited by substantive and persistant uptake of radiometal in the liver. Much current research is focused on performing cancer therapy with Y-90 and Bi-212 chelate-linked mAbs. This report chronicles the development and evaluation of chelating agents for In-111-radioimmunoimaging and Y-90- and Bi-212-radioimmunotherapy. RP GANSOW, OA (reprint author), NIH,RADIAT ONCOL BRANCH,CHEM SECT,BLDG 10,RM B3B69,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 45 TC 79 Z9 80 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 4 BP 369 EP 381 PG 13 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA FN661 UT WOS:A1991FN66100001 ER PT J AU FERRONI, P MILENIC, DE ROSELLI, M CARRASQUILLO, JA RAUBITSCHEK, A SCHLOM, J COLCHER, D AF FERRONI, P MILENIC, DE ROSELLI, M CARRASQUILLO, JA RAUBITSCHEK, A SCHLOM, J COLCHER, D TI POTENTIAL ARTIFACT FOR THE INCREASE OF TUMOR ASSOCIATED ANTIGENS IN SERUM SAMPLES FROM PATIENTS INJECTED WITH MONOCLONAL-ANTIBODIES SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2ND INTERNATIONAL CONF ON DIAGNOSIS AND THERAPY WITH MONOCLONAL ANTIBODIES CY APR 26-28, 1990 CL NAPLES, ITALY ID HETEROPHILIC ANTIBODIES; COLORECTAL-CARCINOMA; COLON CANCER; MAB B72.3; RADIOLOCALIZATION; RADIOIMMUNOASSAY; ELEVATION; THERAPY; IGG AB The administration of [(131)1]B72.3 MAb for both diagnostic and therapeutic purposes is followed by the development of human anti-mouse antibodies (HAMA) in approx. 70% of patients receiving i.v. doses of MAb above 1 mg. The presence of HAMA in circulation interferes in the detection of the tumor-associated glycoprotein TAG-72 using the CA 72-4 RIA. We have reported elsewhere (Ferroni et al., 1990) that heat treatment at 90-degrees-C, pH 6.5, for 15 min is capable of precipitating and/or inactivating HAMA without causing any significant loss in antigen recovery. Furthermore, the removal of the interfering activity leads to a more correct evaluation of TAG-72 serum levels in HAMA-positive serum samples and to a continued use of TAG-72 in the follow-up of patients undergoing MAb-based clinical protocols. C1 NCI,TUMOR IMMUNOL & BIOL LAB,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NCI,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. NCI,ACRF,CTR CLIN,DEPT NUCL MED,BETHESDA,MD 20892. RI Carrasquillo, Jorge/E-7120-2010; Ferroni, Patrizia/C-2705-2017; OI Ferroni, Patrizia/0000-0002-9877-8712; Carrasquillo, Jorge/0000-0002-8513-5734 NR 27 TC 0 Z9 0 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 4 BP 383 EP 387 PG 5 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA FN661 UT WOS:A1991FN66100002 ER PT J AU ROSELLI, M SCHLOM, J GANSOW, OA BRECHBIEL, MW MIRZADEH, S PIPPIN, CG MILENIC, DE COLCHER, D AF ROSELLI, M SCHLOM, J GANSOW, OA BRECHBIEL, MW MIRZADEH, S PIPPIN, CG MILENIC, DE COLCHER, D TI COMPARATIVE BIODISTRIBUTION STUDIES OF DTPA-DERIVATIVE BIFUNCTIONAL CHELATES FOR RADIOMETAL LABELED MONOCLONAL-ANTIBODIES SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2ND INTERNATIONAL CONF ON DIAGNOSIS AND THERAPY WITH MONOCLONAL ANTIBODIES CY APR 26-28, 1990 CL NAPLES, ITALY ID COLON CARCINOMA XENOGRAFTS; GLYCOPROTEIN TAG-72; CANCER-PATIENTS; COLORECTAL-CANCER; ANTI-CEA; B72.3; ANTIBODY-B72.3; REACTIVITY; MICE; ADENOCARCINOMAS AB Biodistribution of five different backbone-substituted derivatives of SCN-Bz-DTPA (1B4M-DTPA, 1M3B-DTPA, 1B3M-DTPA, GEM-DTPA and 2B-DTPA) linked to MAb B72.3 were compared to that of the parent molecule after labeling with indium-111. Athymic mice, bearing human colon carcinoma xenografts (LS-174T) were injected i.v. to determine the biodistribution of the MAb chelate conjugates. Three of the MAb metal chelate conjugates (1B4M-DTPA, 1M3B-DPTA, and 1B3M-DTPA), labeled with In-111 showed efficient and stable tumor localization as well as a slower blood clearance rate than SCN-Bz-DTPA, GEM-DTPA or 2B-DTPA MAb chelate conjugates. Major differences were also seen in normal organ uptake, especially liver and spleen. Tumor-to-liver ratios rose as a function of time for 1B4M-DTPA, 1M3B-DTPA and 1B3M-DTPA MAb chelate conjugates with virtually no accumulation of the radiometal into this organ, as revealed by no increase in the liver-to-blood values. Small accretion in normal liver was noted for SCN-Bz-DTPA, GEM-DTPA or 2B-DTPA MAb chelate conjugates. The results reviewed here, and described previously (Roselli et al., 1991) demonstrate that the use in vivo of backbone-substituted forms of the SCN-Bz-DTPA, such as 1B4M-DTPA, 1M3B-DTPA, and 1B3M-DTPA bound to MAbs, can reduce uptake of indium to normal organs while maximizing the dose to tumor. C1 NCI,TUMOR IMMUNOL & BIOL LAB,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NCI,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. NR 40 TC 40 Z9 41 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 4 BP 389 EP 394 PG 6 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA FN661 UT WOS:A1991FN66100003 ER PT J AU COLCHER, D MILENIC, DE FERRONI, P ROSELLI, M SCHLOM, J AF COLCHER, D MILENIC, DE FERRONI, P ROSELLI, M SCHLOM, J TI INVIVO AND INVITRO CLINICAL-APPLICATIONS OF MONOCLONAL-ANTIBODIES AGAINST TAG-72 SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2ND INTERNATIONAL CONF ON DIAGNOSIS AND THERAPY WITH MONOCLONAL ANTIBODIES CY APR 26-28, 1990 CL NAPLES, ITALY ID CARCINOEMBRYONIC ANTIGEN LEVELS; GLYCOPROTEIN TAG-72; CANCER-PATIENTS; CA-72-4 RADIOIMMUNOASSAY; IMMUNORADIOMETRIC ASSAY; OVARIAN CARCINOMAS; MALIGNANT-MELANOMA; COLORECTAL-CANCER; SEROUS EFFUSIONS; PLEURAL FLUID AB Monoclonal antibodies against a tumor-associated antigen (TAG-72) with mucin-like properties have been generated. MAb B72.3 was used to identify and help characterize this antigen. B72.3 has been successfully used for the localization of tumor metastases in situ after i.v. administration. MAb B72.3 has also been used in conjunction with CC49, another anti-TAG-72 MAb to measure TAG-72 levels in sera and effusions. TAG-72 can be found in the fluids of patients with adenocarcinomas from many different sites. This CA 72-4 double determinant radioimmunoassay in conjunction with assays for carcinoembryonic antigen can identify patients with malignancies with greater sensitivity than either assay alone. C1 NCI,BETHESDA,MD 20892. RI Ferroni, Patrizia/C-2705-2017 OI Ferroni, Patrizia/0000-0002-9877-8712 NR 46 TC 10 Z9 10 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 4 BP 395 EP 401 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA FN661 UT WOS:A1991FN66100004 ER PT J AU GUADAGNI, F SCHLOM, J GREINER, JW AF GUADAGNI, F SCHLOM, J GREINER, JW TI INVITRO AND INVIVO REGULATION OF TUMOR-ANTIGEN EXPRESSION BY HUMAN RECOMBINANT INTERFERONS SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2ND INTERNATIONAL CONF ON DIAGNOSIS AND THERAPY WITH MONOCLONAL ANTIBODIES CY APR 26-28, 1990 CL NAPLES, ITALY ID MONOCLONAL-ANTIBODY B72.3; CARCINOMA CELL-POPULATIONS; ENHANCED EXPRESSION; GAMMA-INTERFERON; MAMMARY-TUMOR; HLA ANTIGENS; HUMAN-BREAST; COLON; HETEROGENEITY; MODULATION AB In vitro treatment with either type I or type II interferon (IFN) can selectively enhance the expression of several tumor antigens, such as the carcinoembryonic antigen (CEA) and the tumor-associated glyco-protein-72 (TAG-72) in different human carcinoma cell lines and result in enhanced level of monoclonal antibody (MAb) binding to the cell surface. In vivo animal studies demonstrated that treatment of athymic mice with a type I interferon [i.e. interferon-alpha(A)] significantly increased the expression of a 90 kDa tumor antigen which improved the targeting of a MAb to the carcinoma xenograft. More recent studies reported that in vitro IFN treatment of human adenocarcinoma cells isolated from human malignant serous effusions selectively increased the expression of TAG-72 and CEA. One can envision that the ability of these cytokines to upregulate the level of expression of human tumor antigens presents an important experimental model in which to study the regulation of markers often correlated with epithelial cell differentiation. In addition, the increase of selective MAb-defined antigens may also be exploited in an adjuvant setting to localize higher amounts of MAbs to the tumor cell surface and, thereby, improve the effectiveness of a MAb for tumor diagnosis and, possibly, therapy. C1 NCI,TUMOR IMMUNOL & BIOL LAB,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 30 TC 10 Z9 10 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 4 BP 409 EP 412 PG 4 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA FN661 UT WOS:A1991FN66100006 ER PT J AU SCHLOM, J MILENIC, DE ROSELLI, M COLCHER, D BIRD, R JOHNSON, S HARDMAN, KD GUADAGNI, F GREINER, JW AF SCHLOM, J MILENIC, DE ROSELLI, M COLCHER, D BIRD, R JOHNSON, S HARDMAN, KD GUADAGNI, F GREINER, JW TI NEW CONCEPTS IN MONOCLONAL-ANTIBODY BASED RADIOIMMUNODIAGNOSIS AND RADIOIMMUNOTHERAPY OF CARCINOMA SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2ND INTERNATIONAL CONF ON DIAGNOSIS AND THERAPY WITH MONOCLONAL ANTIBODIES CY APR 26-28, 1990 CL NAPLES, ITALY ID TUMOR-ASSOCIATED ANTIGENS; ATHYMIC MICE; HUMAN-BREAST; RECOMBINANT; INTERFERON; CELLS; EXPRESSION; INVIVO; PROTEINS; SPECTRUM AB It is now generally agreed that while numerous monoclonal antibodies (MAbs) have been shown to efficiently target tumors in patients, much still needs to be accomplished to optimize MAb based tumor targeting and the use of MAbs in the therapy of human carcinoma. This article will review some recent studies undertaken in our laboratory in an attempt to generate novel recombinant constructs and test new principles to aid in optimizing MAb based diagnosis and therapy. Three areas will be covered: (a) the analysis of dose fractionation protocols; (b) the generation of recombinant/chimeric (rec/chi) MAbs including the generation of a single chain antigen binding protein (SCA); and (c) the use of recombinant interferons (rec IFNs) to selectively up-regulate tumor antigen expression. Each of these topics has been perviously described in detail and appropriate references to these articles are included. C1 GENEX CORP,GAITHERSBURG,MD 20877. RP SCHLOM, J (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 27 TC 21 Z9 21 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 4 BP 425 EP 435 PG 11 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA FN661 UT WOS:A1991FN66100008 ER PT J AU MERINO, MJ MONTEAGUDO, C NEUMANN, RD AF MERINO, MJ MONTEAGUDO, C NEUMANN, RD TI MONOCLONAL-ANTIBODIES FOR RADIOIMMUNOSCINTIGRAPHY OF BREAST-CANCER SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article; Proceedings Paper CT 2ND INTERNATIONAL CONF ON DIAGNOSIS AND THERAPY WITH MONOCLONAL ANTIBODIES CY APR 26-28, 1990 CL NAPLES, ITALY ID EPITHELIAL MEMBRANE ANTIGEN; INTERMEDIATE FILAMENT PROTEINS; ASPIRATION BIOPSY SPECIMENS; MULTIDRUG RESISTANCE GENE; DUCTAL CARCINOMA-INSITU; MALIGNANT HUMAN-BREAST; RAS P21 EXPRESSION; BASEMENT-MEMBRANE; LAMININ RECEPTOR; IMMUNOPEROXIDASE LOCALIZATION AB Breast cancer is the leading cause of cancer deaths among females, and it is estimated that each year, one in ten American women will be newly diagnosed as having the disease. It is therefore not surprising, that a great deal of effort has been made to better understand the biology of breast cancer, and that investigators keep up the search for new tools to better characterize, diagnose and treat these tumours. In this regard, the introduction of the hybridoma technique in 1975 by Kohler and Milstein has lead to an extensive work in the characterization of monoclonal and polyclonal antibodies against breast cancers. A large number of antibodies has been raised to different epitopes present in normal and neoplastic breast tissue; but unfortunately we have yet to find a highly sensitive and specific monoclonal antibody for breast cancer that can successfully be used for scintigraphic detection of nodal metastases and for radioimmunotherapy treatment of this disease. As possible radioimmunodiagnostics, antibodies are known which react with the following antigens: (1) cytoskeletal proteins (2) breast cell products (3) steroid receptors (4) putative tumor-associated antigens (5) oncogene products (6) pregnancy-related products (7) basement membrane antigens (8) degradative enzymes (9) cell receptors for extracellular matrix molecules (10) multidrug resistance gene product (p-glycoprotein) (11) proliferative markers. C1 NIH,DEPT NUCL MED,10-1C-495,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NIH,PATHOL LAB,BETHESDA,MD 20892. RI Monteagudo, Carlos/H-6555-2016 NR 108 TC 7 Z9 7 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 4 BP 437 EP 443 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA FN661 UT WOS:A1991FN66100009 ER PT J AU PAK, KY NEDELMAN, MA FOGLER, WE TAM, SH WILSON, E VANHAARLEM, LJM COLOGNOLA, R WARNAAR, SO DADDONA, PE AF PAK, KY NEDELMAN, MA FOGLER, WE TAM, SH WILSON, E VANHAARLEM, LJM COLOGNOLA, R WARNAAR, SO DADDONA, PE TI EVALUATION OF THE 323/A3 MONOCLONAL-ANTIBODY AND THE USE OF TC-99M-LABELED 323/A3 FAB' FOR THE DETECTION OF PAN ADENOCARCINOMA SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article ID CARCINOMA-ASSOCIATED ANTIGEN; COLON-CARCINOMA; PROTEINS; AFFINITY; BINDING; GLYCOPROTEIN; FRAGMENTS; F(AB')2; THERAPY; 17-1A AB The 323/A3 murine monoclonal antibody, initially described as reactive to breast carcinomas, is found by immunohistological analyses to have broad cross reactivity with adenocarcinomas of diverse histologic origin. The 323/A3 antigen is similar to the tumor-associated 17-1A antigen as revealed by immunoblot and cross-competition cell binding studies. We have investigated the potential use of the 323/A3 monoclonal antibody for tumor imaging as a Fab' molecule labeled with Tc-99m. In vitro studies demonstrate that 323/A3 Fab' has high affinity (2-3 x 10(9) M-1) with no significant loss of immunoreactivity compared to the intact IgG. In vivo studies demonstrate that Tc-99m 323/A3 Fab' can rapidly detect human breast and colon tumor xenografts growing in athymic nude mice. Distinct breast tumor visualization is observed as early as 1 h post intravenous administration with the Tc-99m 323/A3 Fab'. Distinct colon tumor visualization is observed by 3 h (the earliest time point imaged). Tumor-to-blood ratios are higher for Tc-99m 323/A3 Fab' than with a Tc-99m-labeled nonspecific isotype-matched Fab' antibody. These results suggest that Tc-99m 323/A3 Fab' can detect 17-1A antigen and may have potential clinical utility for the rapid diagnostic imaging of adenocarcinomas. C1 NCI,CANC THERAPY EVALUAT PROGRAM,INVEST DRUG BRANCH,ROCKVILLE,MD 20852. CENTOCOR BV,RES & DEV,LEIDEN,NETHERLANDS. RP PAK, KY (reprint author), CENTOCOR INC,DIV RES & DEV,244 GREAT VALLEY PKWY,MALVERN,PA 19355, USA. NR 43 TC 16 Z9 16 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 5 BP 483 EP & PG 0 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA FT388 UT WOS:A1991FT38800007 ER PT J AU ECKELMAN, WC AF ECKELMAN, WC TI THE STATUS OF RADIOPHARMACEUTICAL RESEARCH SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Editorial Material ID CONTRAST AGENT; PET; TOMOGRAPHY C1 NIH,CTR CLIN,BETHESDA,MD 20892. NR 31 TC 7 Z9 7 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PY 1991 VL 18 IS 7 BP R3 EP R6 PG 4 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA GF954 UT WOS:A1991GF95400001 ER PT J AU MARQUEZ, VE BODENTEICH, M AF MARQUEZ, VE BODENTEICH, M TI PSICOPLANOCIN-A - A SYNTHETIC CARBOCYCLIC NUCLEOSIDE WITH THE COMBINED STRUCTURAL FEATURES OF NEPLANOCIN-A AND PSICOFURANINE SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article; Proceedings Paper CT 9TH INTERNATIONAL ROUND TABLE ON NUCLEOSIDES, NUCLEOTIDES, AND THEIR BIOLOGICAL APPLICATIONS CY JUL 30-AUG 03, 1990 CL UPPSALA, SWEDEN ID ACID AB Psicoplanocin A represents the first known example of a carbocyclic ketohexose nucleoside. It was synthesized in 8 steps from racemic cyclopentenone 5 which in turn is available from D-ribonolactone. RP MARQUEZ, VE (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MED CHEM LAB,BETHESDA,MD 20892, USA. NR 11 TC 6 Z9 6 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1991 VL 10 IS 1-3 BP 311 EP 314 DI 10.1080/07328319108046467 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FQ570 UT WOS:A1991FQ57000036 ER PT J AU EGAN, W BOAL, J IYER, RP STORM, C WILSON, SH MEYER, A IVERSEN, P AF EGAN, W BOAL, J IYER, RP STORM, C WILSON, SH MEYER, A IVERSEN, P TI ABASIC OLIGODEOXYRIBONUCLEOSIDE PHOSPHOROTHIOATES AS INHIBITORS OF THE HUMAN IMMUNODEFICIENCY VIRUS-1 (HIV-1) - PHOSPHOROTHIOATE INHIBITION OF HIV-1 REVERSE-TRANSCRIPTASE AND INTERACTIONS WITH SYRIAN-HAMSTER FIBROBLAST (V79) CELLS SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article; Proceedings Paper CT 9TH INTERNATIONAL ROUND TABLE ON NUCLEOSIDES, NUCLEOTIDES, AND THEIR BIOLOGICAL APPLICATIONS CY JUL 30-AUG 03, 1990 CL UPPSALA, SWEDEN ID CHRONICALLY INFECTED-CELLS; OLIGODEOXYNUCLEOTIDES; ANALOGS; KINETICS; INVITRO AB An abasic oligonucleoside phosphosphorothioate has been synthesized and evaluated as an inhibitor of HIV-1 reverse transcriptase. The cellular binding to and mutagenicity of the abasic phosphorothioate with Syrian hampster fibroblasts have also been evaluated. C1 UNIV NEBRASKA,SCH MED,DEPT PHARMACOL,OMAHA,NE 68105. NCI,BETHESDA,MD 20892. RP EGAN, W (reprint author), CTR BIOL EVALUAT & RES,BIOPHYS LAB,BETHESDA,MD 20892, USA. NR 10 TC 2 Z9 2 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1991 VL 10 IS 1-3 BP 457 EP 460 DI 10.1080/07328319108046497 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA FQ570 UT WOS:A1991FQ57000066 ER PT J AU NAKATA, H AF NAKATA, H TI RECENT PROGRESS IN THE PURIFICATION OF ADENOSINE RECEPTORS SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article; Proceedings Paper CT SATELLITE SYMP TO THE 11TH INTERNATIONAL CONGRESS OF PHARMACOLOGY - PHARMACOLOGY OF PURINERGIC RECEPTORS : IMPLICATIONS FOR DRUG DESIGN CY JUL 06-08, 1990 CL NOORDWIJK, NETHERLANDS SP INT UNION PHARM, LEIDEN UNIV, CTR BIO PHARM SCI ID RAT-BRAIN MEMBRANES; AFFINITY-CHROMATOGRAPHY; A1-ADENOSINE RECEPTORS AB A1 adenosine receptors were purified to an apparent homogeneity from rat brain and testicular membranes by a novel affinity chromatography system using xanthine amine congener (XAC) as an immobilized ligand. This affinity chromatography was also useful for the purification of human brain A1 adenosine receptor. RP NAKATA, H (reprint author), NIMH,CLIN SCI LAB,BLDG 10,ROOM 3D-48,BETHESDA,MD 20892, USA. NR 8 TC 1 Z9 1 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1991 VL 10 IS 5 BP 983 EP 991 DI 10.1080/07328319108047236 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA135 UT WOS:A1991GA13500008 ER PT J AU JACOBSON, KA DALY, JW AF JACOBSON, KA DALY, JW TI PURINE FUNCTIONALIZED CONGENERS AS MOLECULAR PROBES FOR ADENOSINE RECEPTORS SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article; Proceedings Paper CT SATELLITE SYMP TO THE 11TH INTERNATIONAL CONGRESS OF PHARMACOLOGY - PHARMACOLOGY OF PURINERGIC RECEPTORS : IMPLICATIONS FOR DRUG DESIGN CY JUL 06-08, 1990 CL NOORDWIJK, NETHERLANDS SP INT UNION PHARM, LEIDEN UNIV, CTR BIO PHARM SCI ID HIGH-AFFINITY; PHOTOAFFINITY CROSSLINKING; A1-ADENOSINE RECEPTORS; BINDING SUBUNIT; RAT-BRAIN; DERIVATIVES; ANALOGS; IDENTIFICATION; ISOPROTERENOL; ANTAGONISTS AB The effects of distal structural changes on biological activity may be studied, using a "functionalized congener" strategy, in which suitably placed chemically reactive chains lead to biologically active conjugates. Probes for photoaffinity labeling, chemical affinity labeling, spectroscopic characterization and affinity chromatography of adenosine receptors have been derived from purine amine congeners (XAC, ADAC for A1-receptors and APEC for A2-receptors). Also, drug conjugates, including prodrugs and lipids, have been designed as potential pharmacological agents. RP JACOBSON, KA (reprint author), NIDDKD,BIOORGAN CHEM LAB,BETHESDA,MD 20892, USA. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 29 TC 14 Z9 15 U1 0 U2 1 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1991 VL 10 IS 5 BP 1029 EP 1038 DI 10.1080/07328319108047240 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA135 UT WOS:A1991GA13500012 ER PT J AU JACOBSON, KA NIKODIJEVIC, O DELACRUZ, D DALY, JW AF JACOBSON, KA NIKODIJEVIC, O DELACRUZ, D DALY, JW TI APEC, AN A2-SELECTIVE ADENOSINE AGONIST, IS A MORE POTENT LOCOMOTOR DEPRESSANT THAN N6-CYCLOHEXYLADENOSINE SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article; Proceedings Paper CT SATELLITE SYMP TO THE 11TH INTERNATIONAL CONGRESS OF PHARMACOLOGY - PHARMACOLOGY OF PURINERGIC RECEPTORS : IMPLICATIONS FOR DRUG DESIGN CY JUL 06-08, 1990 CL NOORDWIJK, NETHERLANDS SP INT UNION PHARM, LEIDEN UNIV, CTR BIO PHARM SCI AB The locomotor depressant effects of APEC are due to activation of central A2 adenosine receptors, while the depressant effects of NECA are dependent primarily on A1-receptor activation. A variety of potent A1-antagonists, including conjugates of the XAC, were screened as antagonists; 2-thio-CPX and CPT reversed effects of CHA, but not of APEC. RP JACOBSON, KA (reprint author), NIDDKD,BIOORGAN CHEM LAB,BETHESDA,MD 20892, USA. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 5 TC 3 Z9 3 U1 0 U2 1 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1991 VL 10 IS 5 BP 1211 EP 1212 DI 10.1080/07328319108047279 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GA135 UT WOS:A1991GA13500051 ER PT J AU BLOCK, G HENSON, DE LEVINE, M AF BLOCK, G HENSON, DE LEVINE, M TI VITAMIN-C - BIOLOGIC FUNCTIONS AND RELATION TO CANCER - SPONSORED BY NATIONAL-CANCER-INSTITUTE AND NATIONAL-INSTITUTE-OF-DIABETES-AND-DIGESTIVE-AND-KIDNEY-DISEASES SEPTEMBER 10-12, 1990, BETHESDA, MARYLAND SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Editorial Material RP BLOCK, G (reprint author), NCI,DIV CANC PREVENT & CONTROL,EPN ROOM 313,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Block, Gladys/E-3304-2010 NR 0 TC 3 Z9 3 U1 0 U2 0 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 SN 0163-5581 J9 NUTR CANCER JI Nutr. Cancer PY 1991 VL 15 IS 3-4 BP 249 EP 250 PG 2 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA FQ285 UT WOS:A1991FQ28500010 ER PT J AU ANDERSEN, AN BLUM, MS JONES, TH AF ANDERSEN, AN BLUM, MS JONES, TH TI VENOM ALKALOIDS IN MONOMORIUM-ROTHSTEINI FOREL REPEL OTHER ANTS - IS THIS THE SECRET TO SUCCESS BY MONOMORIUM IN AUSTRALIAN ANT COMMUNITIES SO OECOLOGIA LA English DT Article DE ALKALOIDS; ANT COMMUNITIES; INTERFERENCE COMPETITION; MONOMORIUM; VENOM ID ARID MALLEE REGION; NORTHWESTERN VICTORIA; GENUS MONOMORIUM; HYMENOPTERA; SOLENOPSIS; FORMICIDAE; POISON; GLAND; FIRE AB Species of the cosmopolitan ant genus Monomorium are small, slow-moving and non-aggressive ants that are extremely successful members of diverse Australian ant communities. Unlike other abundant taxa, they have no obvious specializations enabling them to co-exist with the highly aggressive species of Iridomyrmex that dominate these communities. Here we report results which suggest that Monomorium species owe their success to the topical application of venom alkaloids which repel other ants. The venom alkaloids (trans-2-ethyl-5-undecylpyrrolidine and trans-2-ethyl-5-tridecylpyrrolidine) of Monomorium "rothsteini" were identified and synthesized, and the repellency to other ants of the synthetic alkaloids were tested using bioassays involving the attraction to honey baits of three native species of Iridomyrmex, three tropical "tramp' species, and M. "rothsteini" itself. Repellency to all other ant species was total or nearly so, but only partial to M. "rothsteini". Defensive alkaloids produced from a variety of glands are found in other ant genera, and may be a potent yet poorly appreciated force in interference competition between ant species and thereby the structure of ant communities. C1 UNIV GEORGIA,DEPT ENTOMOL,ATHENS,GA 30602. NHLBI,CHEM LAB,BETHESDA,MD 20892. RP ANDERSEN, AN (reprint author), CSIRO,TROP ECOSYST RES CTR,DIV WILDLIFE & ECOL,PMB 44,WINNELLIE,NT 0821,AUSTRALIA. RI Andersen, Alan/F-8763-2010 NR 32 TC 48 Z9 49 U1 0 U2 5 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0029-8549 J9 OECOLOGIA JI Oecologia PY 1991 VL 88 IS 2 BP 157 EP 160 DI 10.1007/BF00320805 PG 4 WC Ecology SC Environmental Sciences & Ecology GA GM331 UT WOS:A1991GM33100002 PM 28312126 ER PT J AU DUNBAR, CE CROSIER, PS NIENHUIS, AW AF DUNBAR, CE CROSIER, PS NIENHUIS, AW TI INTRODUCTION OF AN ACTIVATED RAS ONCOGENE INTO MURINE BONE-MARROW LYMPHOID PROGENITORS VIA RETROVIRAL GENE-TRANSFER RESULTS IN THYMIC LYMPHOMAS SO ONCOGENE RESEARCH LA English DT Article DE RAS; GENE TRANSFER; LYMPHOMAS; STEM CELLS ID T-CELL RECEPTOR; LONG TERMINAL REPEAT; ACUTE MYELOID-LEUKEMIA; BETA-CHAIN GENES; TRANSGENIC MICE; SARCOMA-VIRUS; ANTIGEN RECEPTOR; HA-RAS; C-MYC; HEMATOPOIETIC PROGENITORS C1 NHLBI,CLIN HEMATOL BRANCH,BLDG 10,ROOM 7C103,BETHESDA,MD 20892. NR 78 TC 23 Z9 24 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 0890-6467 J9 ONCOGENE RES JI Oncogene Res. PY 1991 VL 6 IS 1 BP 39 EP 51 PG 13 WC Oncology; Genetics & Heredity SC Oncology; Genetics & Heredity GA EY773 UT WOS:A1991EY77300005 PM 1705319 ER PT J AU MORRIS, VL RAO, TR KOZAK, CA GRAY, DA CHAN, ECML CORNELL, TJ TAYLOR, CB JONES, RF MCGRATH, CM AF MORRIS, VL RAO, TR KOZAK, CA GRAY, DA CHAN, ECML CORNELL, TJ TAYLOR, CB JONES, RF MCGRATH, CM TI CHARACTERIZATION OF INT-5, A LOCUS ASSOCIATED WITH EARLY EVENTS IN MAMMARY CARCINOGENESIS SO ONCOGENE RESEARCH LA English DT Article DE INT-5; CHROMOSOME MAPPING; HYPERPLASTIC ALVEOLAR NODULES; MOUSE MAMMARY TUMOR VIRUS ID BALB-C MICE; TUMOR VIRUS; ONCOGENE INT-1; MESSENGER-RNA; PROVIRAL ACTIVATION; REPLACEMENT VECTORS; PROTO-ONCOGENE; V-CBL; MOUSE; DNA C1 UNIV TEXAS,HLTH SCI CTR,DEPT OBSTET GYNECOL,SAN ANTONIO,TX 78284. NIAID,BETHESDA,MD 20892. UNIV OTTAWA,DEPT MED,OTTAWA K1H 8M5,ONTARIO,CANADA. MICHIGAN CANC FDN,DETROIT,MI 48201. GRACE BIOONCOL LAB & INST,PONTIAC,MI 48057. RP MORRIS, VL (reprint author), UNIV WESTERN ONTARIO,DEPT MICROBIOL & IMMUNOL,LONDON N6A 5C1,ONTARIO,CANADA. FU NCI NIH HHS [CA44901] NR 41 TC 22 Z9 22 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 0890-6467 J9 ONCOGENE RES JI Oncogene Res. PY 1991 VL 6 IS 1 BP 53 EP 63 PG 11 WC Oncology; Genetics & Heredity SC Oncology; Genetics & Heredity GA EY773 UT WOS:A1991EY77300006 PM 1705320 ER PT J AU TSUCHIMOCHI, M HOSAIN, F ENGELKE, W ZEICHNER, SJ RUTTIMANN, UE WEBBER, RL AF TSUCHIMOCHI, M HOSAIN, F ENGELKE, W ZEICHNER, SJ RUTTIMANN, UE WEBBER, RL TI STUDIES ON FOCAL ALVEOLAR BONE HEALING WITH TECHNETIUM (TC)-99M LABELED METHYLENE DIPHOSPHONATE AND GOLD-COLLIMATED CADMIUM TELLURIDE PROBE SO ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY LA English DT Note ID SEEKING RADIOPHARMACEUTICAL UPTAKE; PERIODONTAL-DISEASE; BEAGLE DOGS AB The benefit of using a collimator for a miniaturized cadmium telluride probe was evaluated by monitoring the bone-healing processes for 13 weeks after the induction of small iatrogenic alveolar bone lesions in one side of the mandible in beagles. Technetium (Tc)-99m labeled methylene diphosphonate (200 to 300 MBq, 5.1 to 8.1 mCi, in a solution of 0.5 to 1 ml, intravenously) was used as a bone-seeking radiopharmaceutical. The radioactivity over the bone lesion (L) and the contralateral normal site (C) in the mandible were measured between 1.5 and 2 hours after injection of the tracer, and the activity ratio L/C served as an index of relative bone uptake. A study of six dogs revealed that the healing response to a hemispheric bone defect of 2 mm diameter in the cortical bone could not be detected by an uncollimated probe, and in a repeated study in two dogs in the use of a gold collimator (5 mm in diameter, 5 mm in length) did not increase the L/C ratio significantly. A second study in six dogs with 5 mm lesions showed that although systematic trends in the time courses of the L/C ratio obtained both with and without the collimator could be demonstrated, the L/C ratio of collimated versus uncollimated measurements was significantly (p < 0.005) increased. In three of the latter six dogs, abscesses developed after 9 weeks, leading to a second increase (p < 0.05) of the L/C ratio with collimation compared with the noninflammation group; without collimation no significant (p > 0.15) difference between the two groups could be demonstrated. Hence the use of a collimator is critical for the detection of radionuclide uptake in small focal defects. The smallest size of noninflammatory lesions that could be reliably detected was 5 mm in diameter, whereas 2 mm lesions were missed. C1 UNIV CONNECTICUT, CTR HLTH, DEPT NUCL MED, FARMINGTON, CT 06032 USA. NIDR, DIAGNOST SYST BRANCH, BETHESDA, MD 20892 USA. RP NIPPON DENT UNIV, SCH DENT NIIGATA, DEPT ORAL & MAXILLOFACIAL SURG 2, 1-8 HAMAURA-CHO, NIIGATA 951, JAPAN. NR 27 TC 5 Z9 5 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 1079-2104 EI 1528-395X J9 ORAL SURG ORAL MED O JI Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. PD JAN PY 1991 VL 71 IS 1 BP 110 EP 115 DI 10.1016/0030-4220(91)90536-L PG 6 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA EU075 UT WOS:A1991EU07500024 PM 1994312 ER PT J AU MALAWER, MM AF MALAWER, MM TI TUMORS OF THE SHOULDER GIRDLE - TECHNIQUE OF RESECTION AND DESCRIPTION OF A SURGICAL CLASSIFICATION SO ORTHOPEDIC CLINICS OF NORTH AMERICA LA English DT Article ID TIKHOFF-LINBERG PROCEDURE; BONE C1 GEORGE WASHINGTON UNIV,SCH MED,WASHINGTON,DC 20052. NCI,SURG BRANCH,BETHESDA,MD 20892. RP MALAWER, MM (reprint author), CHILDRENS HOSP,NATL MED CTR,DEPT ORTHOPED,111 MICHIGAN AVE,NW,WASHINGTON,DC 20010, USA. NR 40 TC 27 Z9 31 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0030-5898 J9 ORTHOP CLIN N AM JI Orthop. Clin. North Am. PD JAN PY 1991 VL 22 IS 1 BP 7 EP 35 PG 29 WC Orthopedics SC Orthopedics GA EW951 UT WOS:A1991EW95100003 PM 1992436 ER PT B AU GRACELY, RH AF GRACELY, RH BE CASEY, KL TI THEORETICAL AND PRACTICAL ISSUES IN PAIN ASSESSMENT IN CENTRAL PAIN SYNDROMES SO PAIN AND CENTRAL NERVOUS SYSTEM DISEASE: THE CENTRAL PAIN SYNDROMES SE BRISTOL-MYERS SQUIBB SYMPOSIUM ON PAIN RESEARCH SERIES LA English DT Proceedings Paper CT 1ST ANNUAL BRISTOL-MYERS SQUIBB SYMP ON PAIN RESEARCH - PAIN AND CENTRAL NERVOUS SYSTEM DISEASE : THE CENTRAL PAIN SYNDROMES CY JUL 12-14, 1990 CL UNIV MICHIGAN, ANN ARBOR, MI SP BRISTOL MYERS SQUIBB HO UNIV MICHIGAN RP GRACELY, RH (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,CLIN PAIN SECT,10-1N-103D,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-776-0 J9 SYMP PAIN R PY 1991 BP 85 EP 101 PG 17 WC Anesthesiology; Neurosciences; Pathology SC Anesthesiology; Neurosciences & Neurology; Pathology GA BT68Z UT WOS:A1991BT68Z00007 ER PT B AU DUBNER, R AF DUBNER, R BE CASEY, KL TI NEURONAL PLASTICITY IN THE SPINAL AND MEDULLARY DORSAL HORNS - A POSSIBLE ROLE IN CENTRAL PAIN MECHANISMS SO PAIN AND CENTRAL NERVOUS SYSTEM DISEASE: THE CENTRAL PAIN SYNDROMES SE BRISTOL-MYERS SQUIBB SYMPOSIUM ON PAIN RESEARCH SERIES LA English DT Proceedings Paper CT 1ST ANNUAL BRISTOL-MYERS SQUIBB SYMP ON PAIN RESEARCH - PAIN AND CENTRAL NERVOUS SYSTEM DISEASE : THE CENTRAL PAIN SYNDROMES CY JUL 12-14, 1990 CL UNIV MICHIGAN, ANN ARBOR, MI SP BRISTOL MYERS SQUIBB HO UNIV MICHIGAN RP DUBNER, R (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 30,ROOM B-18,BETHESDA,MD 20892, USA. NR 0 TC 28 Z9 31 U1 0 U2 1 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-776-0 J9 SYMP PAIN R PY 1991 BP 143 EP 155 PG 13 WC Anesthesiology; Neurosciences; Pathology SC Anesthesiology; Neurosciences & Neurology; Pathology GA BT68Z UT WOS:A1991BT68Z00011 ER PT J AU KATTENBACH, WM PIMENTA, PFP DESOUZA, W DASILVA, PP AF KATTENBACH, WM PIMENTA, PFP DESOUZA, W DASILVA, PP TI GIARDIA-DUODENALIS - A FREEZE-FRACTURE, FRACTURE-FLIP AND CYTOCHEMISTRY STUDY SO PARASITOLOGY RESEARCH LA English DT Article ID AXENIC CULTIVATION; CELL-SURFACE; LAMBLIA; TROPHOZOITES; IDENTIFICATION; MECHANISMS; MEMBRANES; BINDING; LECTIN; MURIS AB The freeze-fracture technique was used to study the structural organization of the membranes of trophozoites of the protozoon Giardia duodenalis. No special array of intramembranous particles was observed in the membrane lining the protozoon body or the flagella. A large globular protuberance located in the ventral region displayed several small circular indentations similar to those seen in the dorsal region. These also occurred on the parasite surface as revealed in fracture-flip replicas. A large number of vesicles were observed below the plasma membrane; they corresponded to an acidic compartment as indicated by fluorescence microscopy of acridine orange-stained cells and contained acid phosphatase as indicated by cytochemistry. In addition, gold-labeled macromolecules (albumin, peroxidase, transferrin, and low-density lipoprotein) accumulated in the vesicles. These observations suggest that the peripheral vesicles of trophozoites are part of the endosomal-lysosomal system of G. duodenalis. C1 UNIV FED RIO DE JANEIRO,INST BIOFIS CARLOS CHAGAS FILHO,DEPT PARASITOL & BIOFIS CELULAR,BR-21941 RIO DE JANEIRO,BRAZIL. NCI,MATH BIOL LAB,MEMBRANE BIOL SECT,FREDERICK,MD 21701. NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. NR 30 TC 17 Z9 17 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0044-3255 J9 PARASITOL RES JI Parasitol. Res. PY 1991 VL 77 IS 8 BP 651 EP 658 DI 10.1007/BF00928678 PG 8 WC Parasitology SC Parasitology GA GM797 UT WOS:A1991GM79700002 PM 1805207 ER PT J AU NECKERS, LM AF NECKERS, LM TI REGULATION OF TRANSFERRIN RECEPTOR EXPRESSION AND CONTROL OF CELL-GROWTH SO PATHOBIOLOGY LA English DT Article DE TRANSFERRIN RECEPTOR; PROLIFERATION; GROWTH FACTORS; CELL CYCLE; CALCIUM; PHORBOL ESTERS; CYCLIC NUCLEOTIDES ID HUMAN LYMPHOCYTE-T; PHORBOL ESTER; LEUKEMIA-CELLS; K562 CELLS; ACTIVATION; DIFFERENTIATION; PROLIFERATION; INDUCTION; CALCIUM; IRON AB Transferrin receptor expression is vital for the continuous growth of most cells. Although iron plays a key role in modulating transferrin receptor expression, other physiological factors are also capable of affecting receptor expression. In hematopoietic cells, these include interleukin-2, interleukin-6, calcium channels, cyclic nucleotides, phorbol esters and viruses. In this review, we will describe how these agents can alter transferrin receptor expression at the levels of transcription, translation and receptor re-cycling. RP NECKERS, LM (reprint author), NCI,MED BRANCH,BETHESDA,MD 20892, USA. NR 24 TC 45 Z9 45 U1 1 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1015-2008 J9 PATHOBIOLOGY JI Pathobiology PD JAN-FEB PY 1991 VL 59 IS 1 BP 11 EP 18 DI 10.1159/000163610 PG 8 WC Cell Biology; Pathology SC Cell Biology; Pathology GA FC400 UT WOS:A1991FC40000003 PM 1645975 ER PT J AU CHONG, YM SO, LY FOK, TF GERLIS, LM AF CHONG, YM SO, LY FOK, TF GERLIS, LM TI CYANOTIC ATRIAL SEPTAL-DEFECT IN A PREMATURE-INFANT SO PEDIATRIC CARDIOLOGY LA English DT Letter ID PULMONARY-HYPERTENSION C1 CHINESE UNIV HONG KONG,PRINCE WALES HOSP,DEPT MORBID ANAT,SHA TIN,HONG KONG. NHLBI,BETHESDA,MD 20892. RP CHONG, YM (reprint author), CHINESE UNIV HONG KONG,PRINCE WALES HOSP,DEPT PAEDIAT,SHA TIN,HONG KONG. NR 3 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0172-0643 J9 PEDIATR CARDIOL JI Pediatr. Cardiol. PD JAN PY 1991 VL 12 IS 1 BP 63 EP 63 DI 10.1007/BF02238506 PG 1 WC Cardiac & Cardiovascular Systems; Pediatrics SC Cardiovascular System & Cardiology; Pediatrics GA EQ140 UT WOS:A1991EQ14000018 PM 1997990 ER PT J AU MCCULLOUGH, DC LEVY, LM DICHIRO, G JOHNSON, DL AF MCCULLOUGH, DC LEVY, LM DICHIRO, G JOHNSON, DL TI TOWARD THE PREDICTION OF NEUROLOGICAL INJURY FROM TETHERED SPINAL-CORD - INVESTIGATION OF CORD MOTION WITH MAGNETIC-RESONANCE SO PEDIATRIC NEUROSURGERY LA English DT Article DE MAGNETIC RESONANCE IMAGING; MYELOMENINGOCELE; OCCULT SPINAL DYSRAPHISM; TETHERED SPINAL CORD ID CHILDREN AB Experience with spinal dysraphism has taught that spinal cord tethering will eventually produce neurological deficit. The primary diagnostic and monitoring tool, magnetic resonance (MR) scanning, almost always produces images consistent with cord tethering, even in asymptomatic or improved, previously operated patients. This presents a problem in selecting appropriate candidates for prophylactic intervention. Ideally, a method of monitoring should predict functional deterioration. Real-time ultrasonography, electrophysiological tests, and phase-motion MR studies could potentially be adapted for this purpose. The authors have evaluated the latter technique in both symptomatic and asymptomatic dysraphic patients with tethering. Studies of cervical cord motion show a clear separation between results in patients with cord expansion from hydromyelia and those with pure tethering. Symptomatic patients with progressive deficit or pain have limited cervical cord motion which improves after surgical untethering. MR studies of cord motion have definite promise in the prediction of deficit from spinal cord tethering and should be further evaluated in comparison with ultrasound and electrophysiologic methods. C1 JOHNS HOPKINS UNIV HOSP,DEPT RADIOL,BALTIMORE,MD 21205. NIH,NEUROIMAGING SECT,BETHESDA,MD 20892. RP MCCULLOUGH, DC (reprint author), GEORGE WASHINGTON UNIV,CHILDRENS HOSP,DEPT NEUROSURG,111 MICHIGAN AVE NW,WASHINGTON,DC 20010, USA. NR 22 TC 2 Z9 2 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1016-2291 J9 PEDIATR NEUROSURG JI Pediatr. Neurosurg. PY 1991 VL 16 IS 1 BP 3 EP 7 PG 5 WC Clinical Neurology; Pediatrics; Surgery SC Neurosciences & Neurology; Pediatrics; Surgery GA GQ716 UT WOS:A1991GQ71600001 ER PT J AU STOBIE, PE HANSEN, CT HAILEY, JR LEVINE, RL AF STOBIE, PE HANSEN, CT HAILEY, JR LEVINE, RL TI A DIFFERENCE IN MORTALITY BETWEEN 2 STRAINS OF JAUNDICED RATS SO PEDIATRICS LA English DT Article DE BILIRUBIN; KERNICTERUS; GUNN RATS; JAUNDICE ID BILIRUBIN; BRAIN AB Homozygous Gunn rats lack bilirubin glucuronyltransferase, become jaundiced, and other develop kernicterus, thus providing a model for neonatal hyper-bilirubinemia. Two new, inbred rat strains that carry the Gunn mutation are described. These were developed by breeding the mutant Gunn gene (j) into the RHA/N and ACI/N strains, producing the new lines, which were designated RHA/N-j and ACI/N-j. Liver assay confirmed the absence of transferase activity in jaundiced rats from both of the new strains, but marked differences in mortality between the strains were observed. The mortality of jaundiced RHA/N-j rats through 8 weeks was the same as that of their nonjaundiced littermates (20%). In contrast, mortality of jaundiced ACI/N-j rats was distinctly greater than that of their nonjaundiced littermates (81%) vs 34%, P < .001). Signs of kernicterus such as ataxia were much more frequent in jaundiced ACI/N-j rats than in jaundiced RHA/N-j rats (73% vs 11%, P < .001). Both strains had comparable albumin concentrations through 8 weeks of age. Serum bilirubin concentrations were also comparable, except for a small but significant difference at 20 days of age (ACI/N-j - 294-mu-mol/L, RHA/N-j = 248-mu-mol/L P < .01). Similarly, the bilirubin-to-albumin ratios were comparable except for a significantly higher ratio at 20 days of age in the ACI-N-j rats (ACI/N-j = .07, RHA/N-j = 0.51, < .01). Thus, the RHA/N-j strain is unusual in that the jaundiced animals healthy. Conversely, the ACI/N-j animals demonstrate a high incidence of kernicterus with mortality. This dramatic strain difference implies that factors in addition to deficiency of bilirubin glucuronyltranserase are modulating the susceptibility to bilirubin toxicity. C1 NHLBI,BIOCHEM LAB,BLDG 3,ROOM 106,BETHESDA,MD 20892. NIH,VET RESOURCES BRANCH,BETHESDA,MD 20892. WALTER REED ARMY MED CTR,WASHINGTON,DC 20307. UNIFORMED SERV UNIV HLTH SCI,DEPT PEDIAT,BETHESDA,MD 20814. RI Levine, Rodney/D-9885-2011 NR 22 TC 9 Z9 9 U1 0 U2 0 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD, ELK GROVE VILLAGE, IL 60007-1098 SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD JAN PY 1991 VL 87 IS 1 BP 88 EP 93 PG 6 WC Pediatrics SC Pediatrics GA EQ978 UT WOS:A1991EQ97800015 PM 1898623 ER PT J AU ROTHMAN, RB LONG, JB BYKOV, V XU, H JACOBSON, AE RICE, KC HOLADAY, JW AF ROTHMAN, RB LONG, JB BYKOV, V XU, H JACOBSON, AE RICE, KC HOLADAY, JW TI UP-REGULATION OF THE OPIOID RECEPTOR COMPLEX BY THE CHRONIC ADMINISTRATION OF MORPHINE - A BIOCHEMICAL MARKER RELATED TO THE DEVELOPMENT OF TOLERANCE AND DEPENDENCE SO PEPTIDES LA English DT Article DE MORPHINE TOLERANCE; NALTREXONE; BETA-FUNALTREXAMINE; RECEPTOR UP-REGULATION ID RAT-BRAIN MEMBRANES; BETA-ENDORPHIN ANALGESIA; BINDING-SITES; PHYSICAL-DEPENDENCE; OPIATE RECEPTOR; PHARMACOLOGICAL CHARACTERIZATION; ANTIOPIATE TYR-MIF-1; DOWN-REGULATION; DELTA-RECEPTOR; UP-REGULATION AB Studies conducted after the development of the rapid filtration assay for opiate receptors, and before the recognition of multiple opioid receptors, failed to detect changes in opioid receptors induced by chronic morphine. Recent experiments conducted in our laboratories were designed to examine the hypothesis that only one of several opioid receptor types might be altered by chronic morphine. Using binding surface analysis and irreversible ligands to increase the "resolving power" of the ligand binding assay, the results indicated that chronic morphine increased both the B(max) and K(d) of the opioid receptor complex, labeled with either [H-3][D-Ala2,D-Leu5]enkephalin, [H-3][D-Ala2-MePhe4,Gly-ol5]enkephalin or [H-3]6-desoxy-6-beta-fluoronaltrexone. In the present study rats were pretreated with drugs known to attenuate the development of tolerance and dependence [the irreversible mu-receptor antagonist, beta-funaltrexamine (beta-FNA), and the inhibitor of tryptophan hydroxylase, para-chlorophenylalanine], prior to subcutaneous implantation of morphine pellets. The results demonstrated that 1) unlike chronic naltrexone, beta-FNA failed to upregulate opioid receptors and 2) both beta-funaltrexamine and PCPA pretreatment attenuated the chronic morphine-induced increase in the B(max), but not the K(d), of the opioid receptor complex. These results provide evidence that naltrexone-induced upregulation of the opioid receptor complex might occur indirectly as a consequence of interactions at beta-funaltrexamine-insensitive opioid receptors and that morphine-induced upregulation (increased B(max)) of the opioid receptor complex is a relevant in vitro marker related to the development of tolerance and dependence. These data collectively support the hypothesis that endogenous antiopiate peptides play an important role in the development of tolerance and dependence to morphine. C1 WALTER REED ARMY MED CTR,DIV MED NEUROSCI,NEUROPHARMACOL BRANCH,WASHINGTON,DC 20307. NIDDK,MED CHEM LAB,BETHESDA,MD 20892. MEDICIS CORP,WASHINGTON,DC 20006. RP ROTHMAN, RB (reprint author), NIMH,CLIN SCI LAB,RECEPTOR STUDIES UNIT,BLDG 10-3D41,BETHESDA,MD 20892, USA. NR 83 TC 58 Z9 58 U1 0 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PD JAN-FEB PY 1991 VL 12 IS 1 BP 151 EP 160 DI 10.1016/0196-9781(91)90182-O PG 10 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA EY791 UT WOS:A1991EY79100026 PM 1646998 ER PT J AU SACERDOTE, P WIEDERMANN, CJ WAHL, LM PERT, CB RUFF, MR AF SACERDOTE, P WIEDERMANN, CJ WAHL, LM PERT, CB RUFF, MR TI VISUALIZATION OF CHOLECYSTOKININ RECEPTORS ON A SUBSET OF HUMAN MONOCYTES AND IN RAT SPLEEN SO PEPTIDES LA English DT Article DE MACROPHAGE; NEUROPEPTIDE; RECEPTORS; BINDING; NEUROIMMUNOMODULATION; CHOLECYSTOKININ; AUTORADIOGRAPHY; SPLEEN; DENDRITIC CELLS ID VASOACTIVE INTESTINAL PEPTIDE; BRAIN CCK RECEPTORS; SUBSTANCE-P; MEDIATED CHEMOTAXIS; BINDING; CELLS; NEUROPEPTIDES; LOCALIZATION; ELUTRIATION; RESPONSES AB Direct radioreceptor binding experiments and Scatchard analysis reveal CCK receptors on elutriator purified human peripheral blood monocytes, but not on purified human T cells. The monocyte receptors have a single class of high (0.1 nM) affinity binding sites. A structure-function analysis of monocyte binding by different CCK analogs correlates well with previously demonstrated chemotactic responses in monocytes and receptors in brain tissue. Biochemical cross-linking indicates that the monocyte CCK recognition molecule is comparable in molecular size to that in brain membranes. Utilizing a novel fluoresceinated Texas Red-CCK conjugate we have visualized that up to 20% of human peripheral monocytes bear receptors for CCK. A discrete and anatomically significant distribution of CCK receptors in rat spleen is shown by film autoradiography of tissue sections. A more detailed microscopic analysis identifies a dendritic population of monocyte-derived cells within the periarteriolar lymphocyte sheath (PALS) of the white pulp as the CCK receptor-bearing cell in spleen. The anatomical localization of receptor-bearing cells within the PALS region suggests a role for CCK in the antigen processing and sensitization phases of the immune response via regulatory effects of this peptide on a specific, local macrophage-related cell population. C1 NIMH,CLIN NEUROSCI BRANCH,BRAIN BIOCHEM SECT,BLDG 36,ROOM 2D-30,BETHESDA,MD 20892. NIDR,MICROBIOL & IMMUNOL LAB,CELLULAR IMMUNOL SECT,BETHESDA,MD 20892. NR 54 TC 33 Z9 33 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PD JAN-FEB PY 1991 VL 12 IS 1 BP 167 EP 176 DI 10.1016/0196-9781(91)90184-Q PG 10 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA EY791 UT WOS:A1991EY79100028 PM 2052492 ER PT J AU HILL, JM GOZES, I HILL, JL FRIDKIN, M BRENNEMAN, DE AF HILL, JM GOZES, I HILL, JL FRIDKIN, M BRENNEMAN, DE TI VASOACTIVE-INTESTINAL-PEPTIDE ANTAGONIST RETARDS THE DEVELOPMENT OF NEONATAL BEHAVIORS IN THE RAT SO PEPTIDES LA English DT Article DE VASOACTIVE INTESTINAL PEPTIDE; VIP; NEUROPEPTIDE; NEUROBEHAVIORAL DEVELOPMENT; NEUROTROPHIC FACTOR; DEVELOPMENTAL SIGNS; BRAIN DEVELOPMENT; VIP HYBRID ANTAGONIST ID SLEEP FACTOR; POLYPEPTIDE; BRAIN; GROWTH; VIP AB Based on the demonstrated neurotrophic activity of VIP in vitro, a recently designed VIP antagonist was used to assess the role of this neuropeptide in the behavioral development of rats. Rats received daily subcutaneous injections from birth to day 14. Observations of developmental milestones/behaviors were made daily for 21 days. Of the measures of behavioral development tested, the time to surface right on day 4 and the day of onset for forelim placing, hindlimb placing, forelimb grasping and air righting were significantly retarded by the antagonist. Cotreatment with VIP prevented the antagonist-induced delay. These results suggest that VIP activity is important in the development of select complex motor behaviors. C1 PEPTIDE DESIGN, GERMANTOWN, MD 20874 USA. TEL AVIV UNIV, SACKLER SCH MED, DEPT CHEM PATHOL, TEL AVIV, ISRAEL. NIMH, CLIN SCI LAB, BIOSTAT UNIT, BETHESDA, MD 20892 USA. WEIZMANN INST SCI, DEPT ORGAN CHEM, IL-76100 REHOVOT, ISRAEL. RP NICHHD, DEV NEUROBIOL LAB,NEUROCHEM UNIT,BLDG 36,RM 2A21, 9000 ROCKVILLE PIKE, BETHESDA, MD 20892 USA. NR 30 TC 64 Z9 65 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 EI 1873-5169 J9 PEPTIDES JI Peptides PD JAN-FEB PY 1991 VL 12 IS 1 BP 187 EP 192 DI 10.1016/0196-9781(91)90186-S PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA EY791 UT WOS:A1991EY79100030 PM 2052494 ER PT J AU VANDENBUSSCHE, E SAUNDERS, RC ORBAN, GA AF VANDENBUSSCHE, E SAUNDERS, RC ORBAN, GA TI LESIONS IN MT IMPAIR SPEED DISCRIMINATION PERFORMANCE IN JAPANESE MONKEYS (MACCACA-FUSCATA) SO PERCEPTION LA English DT Meeting Abstract C1 CATHOLIC UNIV LEUVEN,NEURO & PSYCHOFYSIOL LAB,B-3000 LOUVAIN,BELGIUM. NIMH,WASHINGTON,DC 20032. NR 0 TC 1 Z9 1 U1 0 U2 0 PU PION LTD PI LONDON PA 207 BRONDESBURY PARK, LONDON, ENGLAND NW2 5JN SN 0301-0066 J9 PERCEPTION JI Perception PY 1991 VL 20 IS 1 BP 76 EP 77 PG 2 WC Ophthalmology; Psychology; Psychology, Experimental SC Ophthalmology; Psychology GA GC521 UT WOS:A1991GC52100049 ER PT J AU COLLETT, TS SCHWARZ, U SOBEL, EC AF COLLETT, TS SCHWARZ, U SOBEL, EC TI THE INTERACTION OF OCULOMOTOR CUES AND STIMULUS SIZE IN STEREOSCOPIC DEPTH CONSTANCY SO PERCEPTION LA English DT Article ID BINOCULAR DISTANCE PERCEPTION; MOTION PARALLAX; ABSOLUTE-DISTANCE; EYE-MOVEMENTS; VERGENCE; DISPARITY; ACCOMMODATION; PERSPECTIVE; CONVERGENCE; INFORMATION AB In the natural world, observers perceive an object to have a relatively fixed size and depth over a wide range of distances. Retinal image size and binocular disparity are to some extent scaled with distance to give observers a measure of size constancy. The angle of convergence of the two eyes and their accommodative states are one source of scaling information, but even at close range this must be supplemented by other cues. We have investigated how angular size and oculomotor state interact in the perception of size and depth at different distances. Computer-generated images of planar and stereoscopically simulated 3-D surfaces covered with an irregular blobby texture were viewed on a computer monitor. The monitor rested on a movable sled running on rails within a darkened tunnel. An observer looking into the tunnel could see nothing but the simulated surface so that oculomotor signals provided the major potential cues to the distance of the image. Observers estimated the height of the surface, their distance from it, or the stereoscopically simulated depth within it over viewing distances which ranged from 45 cm to 130 cm. The angular width of the images lay between 2 deg and 10 deg. Estimates of the magnitude of a constant simulated depth dropped with increasing viewing distance when surfaces were of constant angular size. But with surfaces of constant physical size, estimates were more nearly independent of viewing distance. At any one distance, depths appeared to be greater, the smaller the angular size of the image. With most observers, the influence of angular size on perceived depth grew with increasing viewing distance. These findings suggest that there are two components to scaling. One is independent of angular size and related to viewing distance. The second-component is related to angular size, and the weighting accorded to it grows with viewing distance. Control experiments indicate that in the tunnel, oculomotor state provides the principal cue to viewing distance. Thus, the contribution of oculomotor signals to depth scaling is gradually supplanted by other cues as viewing distance grows. Binocular estimates of the heights and distances of planar surfaces of different sizes revealed that angular size and viewing distance interact in a similar way to determine perceived size and perceived distance. C1 NEI,SENSORIMOTOR RES LAB,BETHESDA,MD 20892. NR 44 TC 47 Z9 48 U1 0 U2 6 PU PION LTD PI LONDON PA 207 BRONDESBURY PARK, LONDON, ENGLAND NW2 5JN SN 0301-0066 J9 PERCEPTION JI Perception PY 1991 VL 20 IS 6 BP 733 EP 754 DI 10.1068/p200733 PG 22 WC Ophthalmology; Psychology; Psychology, Experimental SC Ophthalmology; Psychology GA HR159 UT WOS:A1991HR15900005 PM 1816533 ER PT J AU COSTA, PT MCCRAE, RR DYE, DA AF COSTA, PT MCCRAE, RR DYE, DA TI FACET SCALES FOR AGREEABLENESS AND CONSCIENTIOUSNESS - A REVISION OF THE NEO PERSONALITY-INVENTORY SO PERSONALITY AND INDIVIDUAL DIFFERENCES LA English DT Article; Proceedings Paper CT 1991 MIDWINTER MEETING OF THE SOC FOR PERSONALITY ASSESSMENT CY MAR 07-09, 1991 CL NEW ORLEANS, LA SP SOC PERSONAL ASSESSMENT ID 5-FACTOR MODEL; BEHAVIOR; DISEASE; TRAITS C1 US OFF PERSONNEL MANAGEMENT,WASHINGTON,DC. RP COSTA, PT (reprint author), NIA,GERONTOL RES CTR,PERSONAL & COGNIT LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. OI Costa, Paul/0000-0003-4375-1712 NR 68 TC 413 Z9 424 U1 9 U2 81 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0191-8869 J9 PERS INDIV DIFFER JI Pers. Individ. Differ. PY 1991 VL 12 IS 9 BP 887 EP 898 DI 10.1016/0191-8869(91)90177-D PG 12 WC Psychology, Social SC Psychology GA GD159 UT WOS:A1991GD15900005 ER PT J AU RAPAKA, RS PORRECA, F AF RAPAKA, RS PORRECA, F TI DEVELOPMENT OF DELTA OPIOID-PEPTIDES AS NONADDICTING ANALGESICS SO PHARMACEUTICAL RESEARCH LA English DT Review DE DELTA-OPIOID PEPTIDES; NONADDICTING ANALGESICS; DRUG DEVELOPMENT; PEPTIDE DRUGS ID MU-MEDIATED ANTINOCICEPTION; MORPHINE-INDUCED ANALGESIA; BINDING SITE INTERACTIONS; RAT-BRAIN; GASTROINTESTINAL TRANSIT; BETA-FUNALTREXAMINE; OPIATE RECEPTORS; HIGH-AFFINITY; SPINAL-CORD; MOUSE AB Although much effort has been devoted to opioid research since the identification of enkephalins, understanding of the physiological importance and mechanisms of action of endogenous opioids lags behind understanding of opiate alkaloids such as morphine. In recent years, several novel approaches have been refined with promise for the successful development of the long-awaited nonaddicting analgesics that act at the opioid delta receptor. The present communication reviews these efforts. C1 UNIV ARIZONA,COLL MED,DEPT PHARMACOL,TUCSON,AZ 85724. RP RAPAKA, RS (reprint author), NIDA,DIV PRECLIN RES,10A-13,ROCKVILLE,MD 20857, USA. NR 87 TC 103 Z9 104 U1 2 U2 3 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0724-8741 J9 PHARMACEUT RES JI Pharm. Res. PD JAN PY 1991 VL 8 IS 1 BP 1 EP 8 DI 10.1023/A:1015809702296 PG 8 WC Chemistry, Multidisciplinary; Pharmacology & Pharmacy SC Chemistry; Pharmacology & Pharmacy GA EU447 UT WOS:A1991EU44700001 PM 1849640 ER PT J AU DALY, JW HIDE, I MULLER, CE SHAMIM, M AF DALY, JW HIDE, I MULLER, CE SHAMIM, M TI CAFFEINE ANALOGS - STRUCTURE-ACTIVITY-RELATIONSHIPS AT ADENOSINE RECEPTORS SO PHARMACOLOGY LA English DT Article DE CAFFEINE; XANTHINES; ADENOSINE RECEPTORS; PHOSPHODIESTERASES ID SPECIES-DIFFERENCES; STRIATAL MEMBRANES; AFFINITY; BRAIN; INHIBITION; PHOSPHODIESTERASE; METHYLXANTHINES; ALKYLXANTHINES; THEOPHYLLINE; ANTAGONISTS AB Caffeine and analogs that contain ethyl, propyl, allyl, propargyl and other substituents in place of methyl at 1-, 3- and 7-positions were antagonists at the two major classes (A1 and A2) of adenosine receptors. Potency at both receptors increased as methyls were replaced with larger substituents. Certain analogs with only one of the three methyl groups of caffeine replaced by larger substituents were somewhat selective for A2 receptors. None of the analogs were particularly selective for A1 receptors. The presence of polar entities in the substituent at the 1- or 7-position was poorly tolerated at adenosine receptors. Activity of caffeine analogs at A1 and A2 adenosine receptors in a variety of systems and cell types is presented and summarized. RP DALY, JW (reprint author), NIDDKD,BIOORGAN CHEM LAB,BLDG 8,ROOM 1A-15,BETHESDA,MD 20892, USA. RI Muller, Christa/C-7748-2014 OI Muller, Christa/0000-0002-0013-6624 NR 29 TC 61 Z9 62 U1 3 U2 6 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0031-7012 J9 PHARMACOLOGY JI Pharmacology PY 1991 VL 42 IS 6 BP 309 EP 321 DI 10.1159/000138813 PG 13 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GE033 UT WOS:A1991GE03300002 PM 1658821 ER PT J AU GETER, B KAUTZ, MA WETHERINGTON, CL RILEY, AL AF GETER, B KAUTZ, MA WETHERINGTON, CL RILEY, AL TI THE EFFECTS OF FOOD SCHEDULE ADAPTATION ON THE ABILITY OF NALOXONE TO SUPPRESS THE ACQUISITION OF SCHEDULE-INDUCED-POLYDIPSIA SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Article DE SCHEDULE-INDUCED POLYDIPSIA; NALOXONE; FOOD ADAPTATION; OPIATES AND DRINKING; OPIATES AND FEEDING ID CONDITIONED TASTE-AVERSIONS; D-AMPHETAMINE; WATER; RAT; DRINKING; MAINTENANCE; STIMULATION; ANTAGONISTS; MORPHINE; BEHAVIOR AB Naloxone suppressed the acquisition of schedule-induced polydipsia (SIP) in rats given no previous exposure to the feeding schedule. Adaptation to the feeding schedule prior to SIP acquisition attenuated this suppression. Specifically, water consumption, bout probability, licks/bout and maximum lick rates during the interpellet interval (IPI) were significantly increased by adaptation. Although adaptation attenuated the suppressive effects of naloxone on SIP, this attenuation was not complete. Adapted, naloxone-treated subjects displayed both decreased water consumption and bout probability as compared to distilled water-treated controls. Unlike the effects of adaptation on naloxone's suppression of SIP, adaptation completely eliminated naloxone's suppression of feeding. That adapted subjects ate at control levels while still displaying a lower level of SIP suggests that the suppressive effect of naloxone on the acquisition of SIP is not an indirect effect of naloxone on feeding, but rather a direct effect of naloxone on developing SIP. Given that naloxone has a general suppressive effect on drinking (including SIP), what remains to be determined is why naloxone has no effect on established SIP. Possible explanations for this are discussed. C1 NIDA,ROCKVILLE,MD 20857. RP GETER, B (reprint author), AMERICAN UNIV,DEPT PSYCHOL,PSYCHOPHARMACOL LAB,WASHINGTON,DC 20016, USA. NR 34 TC 3 Z9 3 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD JAN PY 1991 VL 38 IS 1 BP 85 EP 92 DI 10.1016/0091-3057(91)90593-Q PG 8 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA EQ553 UT WOS:A1991EQ55300013 PM 2017458 ER PT J AU CHOCHUNG, YS CLAIR, T TORTORA, G YOKOZAKI, H AF CHOCHUNG, YS CLAIR, T TORTORA, G YOKOZAKI, H TI ROLE OF SITE-SELECTIVE CAMP ANALOGS IN THE CONTROL AND REVERSAL OF MALIGNANCY SO PHARMACOLOGY & THERAPEUTICS LA English DT Review ID DEPENDENT PROTEIN-KINASE; CYCLIC-AMP-BINDING; MAMMARY-TUMOR REGRESSION; ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE; ADENYLATE-CYCLASE ACTIVITY; BOVINE CARDIAC-MUSCLE; II REGULATORY SUBUNIT; HUMAN-BREAST-CANCER; FRIEND-ERYTHROLEUKEMIC CELLS; RAS ONCOGENE EXPRESSION AB Two isoforms of cAMP receptor protein, RI and RII, the regulatory subunits of cAMP-dependent protein kinase, transduce opposite signals, the RI being stimulatory and the RII being inhibitory of cell proliferation. In normal cells RI and RII exist at a specific physiological ratio whereas in cancer cells such physiological balance of these receptor proteins is disrupted. Reversal and suppression of malignancy can be achieved when the physiologic ratio of these intracellular signal transducers of cAMP is restored as shown by the use of site-selective cAMP analogs, antisense oligodeoxynucleotides or gene transfer, suggesting new approaches to cancer control. RP CHOCHUNG, YS (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,CELLULAR BIOCHEM SECT,BETHESDA,MD 20892, USA. OI Yokozaki, Hiroshi/0000-0001-5276-3331 NR 346 TC 62 Z9 62 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0163-7258 J9 PHARMACOL THERAPEUT JI Pharmacol. Ther. PY 1991 VL 50 IS 1 BP 1 EP 33 DI 10.1016/0163-7258(91)90071-S PG 33 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FW483 UT WOS:A1991FW48300001 PM 1653961 ER PT J AU ROSSI, JJ CANTIN, EM SARVER, N CHANG, PF AF ROSSI, JJ CANTIN, EM SARVER, N CHANG, PF TI THE POTENTIAL USE OF CATALYTIC RNAS IN THERAPY OF HIV-INFECTION AND OTHER DISEASES SO PHARMACOLOGY & THERAPEUTICS LA English DT Review ID ANTI-SENSE RNA; ANTISENSE RNA; MESSENGER-RNA; GENE-EXPRESSION; SELF-CLEAVAGE; TRANSGENIC PLANTS; INHIBITION; VIRUS; CELLS; DNA AB This article describes the applications (both real and potential) of a new antiviral strategy, based on the use of antisense, catalytic RNAs (ribozymes) as therapeutic agents. An understanding of both antisense inhibition of gene expression and RNA autocleavage reactions are essential to the use of this technology. In addition, for the successful application of this technology in clinical settings, an interdisciplinary approach involving clinicians, molecular and cellular biologists, will be necessary. The following treatise will highlight several salient features of ribozyme technology, emphasizing its application as an antiviral as well as discuss some problems and potential solutions pertinent to the clinical application of this technology. C1 CITY HOPE NATL MED CTR,DEPT NEUROL,DUARTE,CA 91010. LOMA LINDA UNIV,SCH MED,DEPT BIOCHEM,LOMA LINDA,CA 92350. NIAID,AIDS PROGRAM,DEV THERAPEUT BRANCH,ROCKVILLE,MD 20852. RP ROSSI, JJ (reprint author), CITY HOPE NATL MED CTR,BECKMAN RES INT,DEPT MOLEC GENET,DUARTE,CA 91010, USA. FU NIAID NIH HHS [AI25959, AI29329] NR 43 TC 31 Z9 31 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0163-7258 J9 PHARMACOL THERAPEUT JI Pharmacol. Ther. PY 1991 VL 50 IS 2 BP 245 EP 254 DI 10.1016/0163-7258(91)90016-F PG 10 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GB124 UT WOS:A1991GB12400004 PM 1763136 ER PT J AU KOHN, KW AF KOHN, KW TI PRINCIPLES AND PRACTICE OF DNA FILTER ELUTION SO PHARMACOLOGY & THERAPEUTICS LA English DT Review ID SINGLE-STRAND BREAKS; ALKALI-LABILE SITES; SALMONELLA-HEPATOCYTE SYSTEM; XERODERMA PIGMENTOSUM-CELLS; MOUSE LEUKEMIA-L1210 CELLS; PROTEIN CROSS-LINKING; BASE EXCISION REPAIR; RAT TESTICULAR DNA; MAMMALIAN-CELLS; CYTO-TOXICITY AB DNA filter elution assays have proved useful in studies of DNA strand breaks and crosslinks produced in mammalian cells or tissues by a wide variety of carcinogenic and cancer chemotherapeutic agents. The basic types of DNA lesions that can be measured include single and double-strand breaks, interstrand crosslinks and DNA-protein crosslinks. DNA filter elution has also been adapted to the assay of other lesions such as alkali-labile sites and the protein-associated strand breaks of topoisomerase DNA cleavage complexes. The essential concepts and theory of the technique are discussed and the applications of the technique to various types of studies are critically reviewed. RP KOHN, KW (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MOLEC PHARMACOL LAB,BETHESDA,MD 20814, USA. NR 112 TC 146 Z9 148 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0163-7258 J9 PHARMACOL THERAPEUT JI Pharmacol. Ther. PY 1991 VL 49 IS 1-2 BP 55 EP 77 DI 10.1016/0163-7258(91)90022-E PG 23 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FD380 UT WOS:A1991FD38000004 PM 1852788 ER PT J AU SHEARS, SB AF SHEARS, SB TI REGULATION OF THE METABOLISM OF 1,2-DIACYLGLYCEROLS AND INOSITOL PHOSPHATES THAT RESPOND TO RECEPTOR ACTIVATION SO PHARMACOLOGY & THERAPEUTICS LA English DT Review ID PROTEIN-KINASE-C; PAROTID ACINAR-CELLS; AORTIC SMOOTH-MUSCLE; DIACYLGLYCEROL ACYLTRANSFERASE ACTIVITY; 1,4,5-TRISPHOSPHATE 3-KINASE ACTIVITY; THYROTROPIN-RELEASING-HORMONE; ADRENAL GLOMERULOSA CELLS; EPIDERMAL GROWTH-FACTOR; CEREBRAL-CORTEX SLICES; SECRETING RINM5F CELLS AB This review assimilates information on the regulation of the metabolism of those inositol phosphates and diacylglycerols that respond to receptor activation. Particular emphasis is placed on the regulation of specific enzymes, the occurrence of isoenzymes, and metabolic compartmentalization; the overall aim is to demonstrate the significance of these activities in relation to the physiological impact of the various cell signalling processes. RP SHEARS, SB (reprint author), NIEHS,CELLULAR & MOLEC PHARMACOL LAB,INOSITOL LIPID SECT,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 272 TC 49 Z9 49 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0163-7258 J9 PHARMACOL THERAPEUT JI Pharmacol. Ther. PY 1991 VL 49 IS 1-2 BP 79 EP 104 DI 10.1016/0163-7258(91)90023-F PG 26 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FD380 UT WOS:A1991FD38000005 PM 1649478 ER PT J AU MUZAFFAR, A BROSSI, A AF MUZAFFAR, A BROSSI, A TI CHEMISTRY OF COLCHICINE SO PHARMACOLOGY & THERAPEUTICS LA English DT Review ID ANALOGS AB Chemical structures of colchicine and related analogs, including allo-compounds with a six-membered ring, are presented here with correct absolute configurations, showing the natural representatives as a S-atropisomers. Spectral data, synthesis, biosynthesis, and metabolism of colchicine are discussed. Structural requirements required for these compounds to inhibit polymerization of tubulin and binding of radiolabeled colchicine in vitro are presented. RP MUZAFFAR, A (reprint author), NIDDK,STRUCT BIOL LAB,NAT PROD SECT,BETHESDA,MD 20892, USA. NR 35 TC 11 Z9 11 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0163-7258 J9 PHARMACOL THERAPEUT JI Pharmacol. Ther. PY 1991 VL 49 IS 1-2 BP 105 EP 109 DI 10.1016/0163-7258(91)90024-G PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FD380 UT WOS:A1991FD38000006 PM 1852784 ER PT J AU THORGEIRSSON, SS SILVERMAN, JA GANT, TW MARINO, PA AF THORGEIRSSON, SS SILVERMAN, JA GANT, TW MARINO, PA TI MULTIDRUG RESISTANCE GENE FAMILY AND CHEMICAL CARCINOGENS SO PHARMACOLOGY & THERAPEUTICS LA English DT Article ID MULTIPLE-DRUG RESISTANCE; REGENERATING RAT-LIVER; P-GLYCOPROTEIN GENE; MDR1 GENE; MESSENGER-RNA; AH RECEPTOR; MOUSE-LIVER; COMPLEMENTARY-DNA; CELLS; EXPRESSION AB The data discussed in this review indicate that the coordinated induction of both the mdr gene family and a subfamily of the cytochrome P-450 supergene family provide a unified response of the organism to prevent lethal accumulation of xenobiotics. Consequently, a distinct physiological role for the mdr multigene family now exists. Furthermore, recent evidence suggests the existence of multiple receptors with overlapping substrate specificity that are involved in the induction of both mdr and P-4501A gene families. The increased expression of mdr gene(s) in the early stages of liver carcinogenesis and presumably in other tissues is associated with the development of xenobiotic resistance that is observed in the preneoplastic cell populations. These observations may have important clinical implications and may provide an explanation for resistance to chemotherapy of tumors in organs such as liver and colon that are frequently exposed to both environmental and dietary xenobiotics. RP THORGEIRSSON, SS (reprint author), NCI,DIV CANC ETIOL,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. NR 62 TC 65 Z9 66 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0163-7258 J9 PHARMACOL THERAPEUT JI Pharmacol. Ther. PY 1991 VL 49 IS 3 BP 283 EP 292 DI 10.1016/0163-7258(91)90059-U PG 10 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FN612 UT WOS:A1991FN61200006 PM 1675806 ER PT J AU BRYANT, SG FISHER, S PRINSLEY, DM OLINS, NJ LARSON, DB AF BRYANT, SG FISHER, S PRINSLEY, DM OLINS, NJ LARSON, DB TI EFFECTS OF AGE ON REPORTING OF ADVERSE CLINICAL EVENTS - RESULTS FROM 2 POSTMARKETING SURVEILLANCE METHODS SO PHARMACOTHERAPY LA English DT Article ID DRUG-REACTIONS AB Two postmarketing surveillance methods were employed to determine the possible effects of age on reports by outpatients of adverse clinical events (ACEs) occurring shortly after treatment with targeted oral antibiotics or tricyclic antidepressants. One approach involved staff-initiated interviews of patients by telephone and the other involved patient-initiated telephone calls to report possible adverse drug reactions. Regardless of the method, the interviews obtained ACE reports both spontaneously and by review of organ systems. Both postmarketing methods confirmed monotonic relationships for age with treatment duration on the target drugs and with the number of concurrent medications. However, the expected positive correlation between age and spontaneously reported ACEs was evident only in the self-monitoring patients who initiated reports. Negative correlations between age and the number of reported valid ACEs were seen in six independent samples, whereas only two demonstrated a positive relationship. This disparity may elucidate a critical methodologic problem: careful attention must be paid to the means of eliciting information about possible adverse clinical events before attempting to study adverse drug reactions, which are necessarily a subset of ACEs. C1 UNIV TEXAS,MED BRANCH,DEPT INTERNAL MED,GALVESTON,TX 77550. AARP PHARM SERV,ALEXANDRIA,VA. NIMH,ROCKVILLE,MD 20857. RP BRYANT, SG (reprint author), UNIV TEXAS,MED BRANCH,CTR MEDICAT MONITORING D41,GALVESTON,TX 77550, USA. NR 20 TC 4 Z9 4 U1 0 U2 0 PU PHARMACOTHERAPY PUBLICATIONS INC PI BOSTON PA NEW ENGLAND MEDICAL CENTER BOX 806 171 HARRISON AVE, BOSTON, MA 02111 SN 0277-0008 J9 PHARMACOTHERAPY JI Pharmacotherapy PY 1991 VL 11 IS 3 BP 249 EP 255 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA FT628 UT WOS:A1991FT62800009 PM 1862016 ER PT J AU SALOMON, DR AF SALOMON, DR TI THE USE OF IMMUNOSUPPRESSIVE DRUGS IN KIDNEY-TRANSPLANTATION SO PHARMACOTHERAPY LA English DT Review ID OKT3 MONOCLONAL-ANTIBODY; T-CELL ACTIVATION; CADAVERIC RENAL-TRANSPLANTATION; SYSTEMIC LUPUS-ERYTHEMATOSUS; INTERLEUKIN-2 FUSION PROTEIN; CYCLOSPORIN-A; HEART-TRANSPLANTATION; ALLOGRAFT-REJECTION; THYMOCYTE GLOBULIN; CLINICAL-TRIAL AB The proper management of immunosuppression in transplantation requires a working knowledge of immunology and pharmacology. In practice we employ two to four agents in one of several complicated protocols. If we consider the variability of individual patients, the potential for drug interactions, and significant compliance problems inherent in any long-term multidrug regimen, the dimensions of the challenge of immunosuppression to the practicing clinician become apparent. C1 GEORGETOWN UNIV,MED CTR,DIV NEPHROL,WASHINGTON,DC 20007. RP SALOMON, DR (reprint author), NIAID,IMMUNOL LAB,BLDG 10,RM 11N312,BETHESDA,MD 20892, USA. RI Salomon, Daniel/E-9380-2012 NR 118 TC 4 Z9 4 U1 0 U2 0 PU PHARMACOTHERAPY PUBLICATIONS INC PI BOSTON PA NEW ENGLAND MEDICAL CENTER BOX 806 171 HARRISON AVE, BOSTON, MA 02111 SN 0277-0008 J9 PHARMACOTHERAPY JI Pharmacotherapy PY 1991 VL 11 IS 6 BP S153 EP S164 PG 12 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA GY057 UT WOS:A1991GY05700008 PM 1771141 ER PT B AU CUTLER, RG AF CUTLER, RG BE PIERPAOLI, W FABRIS, N TI HUMAN LONGEVITY AND AGING - POSSIBLE ROLE OF REACTIVE OXYGEN SPECIES SO PHYSIOLOGICAL SENESCENCE AND ITS POSTPONEMENT : THEORETICAL APPROACHES AND RATIONAL INTERVENTIONS: SECOND STROMBOLI CONFERENCE ON AGING AND CANCER SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT 2ND STROMBOLI CONF ON AGING AND CANCER - PHYSIOLOGICAL SENESCENCE AND ITS POSTPONEMENT : THEORETICAL APPROACHES AND RATIONAL INTERVENTIONS CY MAY 28-JUN 01, 1990 CL STROMBOLI, ITALY SP SIGMA TAU, INST RES SENESCENCE, GLENN FDN MED RES, INST MERIEUX, ITALIAN NATL RES CTR AGING, NEW YORK ACAD SCI ID SUPEROXIDE-DISMUTASE; LIFE-SPAN; METABOLIC-RATE; DROSOPHILA; EVOLUTION; EXPRESSION; OLD RP CUTLER, RG (reprint author), NATL INST AGING,GERONTOL RES CTR,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 0 TC 73 Z9 75 U1 1 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-652-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 621 BP 1 EP 28 PG 28 WC Oncology; Endocrinology & Metabolism; Geriatrics & Gerontology; Immunology; Physiology SC Oncology; Endocrinology & Metabolism; Geriatrics & Gerontology; Immunology; Physiology GA BT95D UT WOS:A1991BT95D00001 ER PT B AU SPECTOR, NH AF SPECTOR, NH BE PIERPAOLI, W FABRIS, N TI CONCLUDING REMARKS - 2 DOZEN CURRENT PROBLEMS IN NEUROIMMUNOMODULATION, AGING, AND CANCER-RESEARCH - THE 2ND STROMBOLI COCKTAIL - FURTHER RAMBUNCTIOUS REMARKS SO PHYSIOLOGICAL SENESCENCE AND ITS POSTPONEMENT : THEORETICAL APPROACHES AND RATIONAL INTERVENTIONS: SECOND STROMBOLI CONFERENCE ON AGING AND CANCER SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT 2ND STROMBOLI CONF ON AGING AND CANCER - PHYSIOLOGICAL SENESCENCE AND ITS POSTPONEMENT : THEORETICAL APPROACHES AND RATIONAL INTERVENTIONS CY MAY 28-JUN 01, 1990 CL STROMBOLI, ITALY SP SIGMA TAU, INST RES SENESCENCE, GLENN FDN MED RES, INST MERIEUX, ITALIAN NATL RES CTR AGING, NEW YORK ACAD SCI RP SPECTOR, NH (reprint author), NIH,DIV FUNDAMENTAL NEUROSCI,FED BLDG,ROOM 916,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-652-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 621 BP 441 EP 446 PG 6 WC Oncology; Endocrinology & Metabolism; Geriatrics & Gerontology; Immunology; Physiology SC Oncology; Endocrinology & Metabolism; Geriatrics & Gerontology; Immunology; Physiology GA BT95D UT WOS:A1991BT95D00034 ER PT J AU KESSLER, LG FEUER, EJ BROWN, ML AF KESSLER, LG FEUER, EJ BROWN, ML TI PROJECTIONS OF THE BREAST-CANCER BURDEN TO UNITED-STATES WOMEN - 1990-2000 SO PREVENTIVE MEDICINE LA English DT Article; Proceedings Paper CT WORKSHOP ON ANTIESTROGEN PREVENTION OF BREAST CANCER CY OCT 02-03, 1989 CL MADISON, WI SP NATL CANC INST, AMER CANC SOC ID MORTALITY; MAMMOGRAPHY RP KESSLER, LG (reprint author), NCI,APPL RES BRANCH,9000 ROCKVILLE PIKE,BLDG EPN,ROOM 313,BETHESDA,MD 20892, USA. NR 23 TC 38 Z9 38 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0091-7435 J9 PREV MED JI Prev. Med. PD JAN PY 1991 VL 20 IS 1 BP 170 EP 182 DI 10.1016/0091-7435(91)90018-Y PG 13 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA EV761 UT WOS:A1991EV76100018 PM 2008426 ER PT J AU MCCRAY, AT ARONSON, AR AF MCCRAY, AT ARONSON, AR TI MAPPING THE LANGUAGE OF USERS TO THE LANGUAGE OF DOCUMENTS SO PROCEEDINGS OF THE ASIS ANNUAL MEETING LA English DT Meeting Abstract C1 NATL LIB MED,BETHESDA,MD 20894. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INFORMATION TODAY INC PI MEDFORD PA 143 OLD MARLTON PIKE, MEDFORD, NJ 08055-8750 SN 0044-7870 J9 P ASIS ANNU MEET JI Proc. ASIS Annu. Meet. PY 1991 VL 28 BP 328 EP 329 PG 2 WC Computer Science, Information Systems; Information Science & Library Science SC Computer Science; Information Science & Library Science GA GP789 UT WOS:A1991GP78900046 ER PT J AU MCCRAY, AT AF MCCRAY, AT TI SIG/ALP, SIG/CR AND SIG/MED - THE UNIFIED MEDICAL LANGUAGE SYSTEM SO PROCEEDINGS OF THE ASIS ANNUAL MEETING LA English DT Editorial Material RP MCCRAY, AT (reprint author), NATL LIB MED,BETHESDA,MD 20209, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INFORMATION TODAY INC PI MEDFORD PA 143 OLD MARLTON PIKE, MEDFORD, NJ 08055-8750 SN 0044-7870 J9 P ASIS ANNU MEET JI Proc. ASIS Annu. Meet. PY 1991 VL 28 BP 330 EP 330 PG 1 WC Computer Science, Information Systems; Information Science & Library Science SC Computer Science; Information Science & Library Science GA GP789 UT WOS:A1991GP78900047 ER PT J AU HUMPHREY, S AF HUMPHREY, S TI COMPUTERIZED ASSISTANCE AND KNOWLEDGE BASE MANAGEMENT FOR INDEXING SO PROCEEDINGS OF THE ASIS ANNUAL MEETING LA English DT Meeting Abstract C1 NATL LIB MED,BETHESDA,MD 20894. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INFORMATION TODAY INC PI MEDFORD PA 143 OLD MARLTON PIKE, MEDFORD, NJ 08055-8750 SN 0044-7870 J9 P ASIS ANNU MEET JI Proc. ASIS Annu. Meet. PY 1991 VL 28 BP 335 EP 335 PG 1 WC Computer Science, Information Systems; Information Science & Library Science SC Computer Science; Information Science & Library Science GA GP789 UT WOS:A1991GP78900057 ER PT J AU THOMA, GR AF THOMA, GR TI SIG CR AND SIG LAN - ELECTRONIC DOCUMENT DELIVERY SO PROCEEDINGS OF THE ASIS ANNUAL MEETING LA English DT Editorial Material RP THOMA, GR (reprint author), NATL LIB MED,BETHESDA,MD 20209, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INFORMATION TODAY INC PI MEDFORD PA 143 OLD MARLTON PIKE, MEDFORD, NJ 08055-8750 SN 0044-7870 J9 P ASIS ANNU MEET JI Proc. ASIS Annu. Meet. PY 1991 VL 28 BP 338 EP 338 PG 1 WC Computer Science, Information Systems; Information Science & Library Science SC Computer Science; Information Science & Library Science GA GP789 UT WOS:A1991GP78900064 ER PT J AU THOMA, GR WALKER, FL AF THOMA, GR WALKER, FL TI A PROTOTYPE SYSTEM FOR AUTOMATED DOCUMENT DELIVERY SO PROCEEDINGS OF THE ASIS ANNUAL MEETING LA English DT Meeting Abstract C1 NATL LIB MED,BETHESDA,MD 20894. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INFORMATION TODAY INC PI MEDFORD PA 143 OLD MARLTON PIKE, MEDFORD, NJ 08055-8750 SN 0044-7870 J9 P ASIS ANNU MEET JI Proc. ASIS Annu. Meet. PY 1991 VL 28 BP 338 EP 338 PG 1 WC Computer Science, Information Systems; Information Science & Library Science SC Computer Science; Information Science & Library Science GA GP789 UT WOS:A1991GP78900065 ER PT J AU DAVID, SL AF DAVID, SL TI COMBATING THE DRUG-PROBLEM - REACHING MINORITY POPULATIONS THROUGH MASS-MEDIA, EDUCATION AND LIBRARIES SO PROCEEDINGS OF THE ASIS ANNUAL MEETING LA English DT Meeting Abstract C1 NIDA,COMMUNITY & PROFESS EDUC BRANCH,ROCKVILLE,MD 20857. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INFORMATION TODAY INC PI MEDFORD PA 143 OLD MARLTON PIKE, MEDFORD, NJ 08055-8750 SN 0044-7870 J9 P ASIS ANNU MEET JI Proc. ASIS Annu. Meet. PY 1991 VL 28 BP 357 EP 357 PG 1 WC Computer Science, Information Systems; Information Science & Library Science SC Computer Science; Information Science & Library Science GA GP789 UT WOS:A1991GP78900101 ER PT J AU NELSON, KG TAKAHASHI, T BOSSERT, NL WALMER, DK MCLACHLAN, JA AF NELSON, KG TAKAHASHI, T BOSSERT, NL WALMER, DK MCLACHLAN, JA TI EPIDERMAL GROWTH-FACTOR REPLACES ESTROGEN IN THE STIMULATION OF FEMALE GENITAL-TRACT GROWTH AND DIFFERENTIATION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE UTERUS; VAGINA; DNA SYNTHESIS ID FACTOR-ALPHA; RIBONUCLEIC-ACID; GENE-EXPRESSION; MAMMARY-GLAND; MOUSE UTERUS; RAT; LACTOTRANSFERRIN; SECRETION; PANCREAS; PROTEIN AB The in vivo studies presented here demonstrate that epidermal growth factor (EGF) is an important autocrine and/or paracrine mediator of estrogen-induced growth and differentiation in mouse uterus and vagina. An antibody specific for EGF significantly inhibited estrogen-induced uterine and vaginal growth, thereby implicating EGF involvement in estrogen action. Furthermore, EGF administered via slow-release pellets in ovariectomized mice acted as a potent uterine and vaginal mitogen as well as inducer of vaginal keratinization. Experiments with ovariectomized, adrenalectomized, hypophysectomized mice indicated that EGF mitogenesis does not require pituitary or adrenal hormones. Treatment with EGF also mimicked estrogen in the induction of uterine lactoferrin (a major estrogen-inducible secretory protein) mRNA and protein. These data suggest that EGF has estrogen-like effects in the promotion of cell growth in the reproductive tract and that EGF may serve as an important mediator of estrogen action in vivo. C1 NIEHS,REPROD & DEV TOXICOL LAB,POB 12233,RES TRIANGLE PK,NC 27709. DUKE UNIV,MED CTR,DEPT OBSTET & GYNECOL,DURHAM,NC 27710. NR 31 TC 348 Z9 355 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 1 BP 21 EP 25 DI 10.1073/pnas.88.1.21 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EQ544 UT WOS:A1991EQ54400005 PM 1986369 ER PT J AU DINMAN, JD ICHO, T WICKNER, RB AF DINMAN, JD ICHO, T WICKNER, RB TI A -1 RIBOSOMAL FRAMESHIFT IN A DOUBLE-STRANDED-RNA VIRUS OF YEAST FORMS A GAG POL FUSION PROTEIN SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE RNA POLYMERASE; LACZ FUSION; L-A VIRUS ID ROUS-SARCOMA VIRUS; MURINE LEUKEMIA-VIRUS; NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI; BACTERIOPHAGE-T7 DNA; GENE-EXPRESSION; POLYMERASE; CODONS; SUPPRESSION; TRANSPOSON AB The L-A double-stranded RNA (dsRNA) virus of Saccharomyces cerevisiae has two open reading frames (ORFs). ORF1 encodes the 80-kDa major coat protein (gag). ORF2, which is expressed only as a 180-kDa fusion protein with ORF1, encodes a single-stranded RNA-binding domain and has the consensus sequence for RNA-dependent RNA polymerases of (+)-strand and double-stranded RNA viruses (pol). We show that the 180-kDa protein is formed by -1 ribosomal frameshifting by a mechanism indistinguishable from that of retroviruses. Analysis of the "slippery site" suggests that a low probability of unpairing of the aminoacyl-tRNA from the 0-frame codon at the ribosomal A site reduces the efficiency of frameshifting more than the reluctance of a given tRNA to have its wobble base mispaired. Frameshifting of L-A requires a pseudoknot structure just downstream of the shift site. The efficiency of the L-A frameshift site is 1.8%, similar to the observed molar ratio in viral particles of the 180-kDa fusion protein to the major coat protein. RP DINMAN, JD (reprint author), NIDDKD,BIOCHEM PHARMACOL LAB,GENET SIMPLE EUKARYOTES SECT,BETHESDA,MD 20892, USA. OI Dinman, Jonathan/0000-0002-2402-9698 NR 46 TC 248 Z9 254 U1 2 U2 6 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 1 BP 174 EP 178 DI 10.1073/pnas.88.1.174 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EQ544 UT WOS:A1991EQ54400038 PM 1986362 ER PT J AU KOVACS, G KUNG, HF AF KOVACS, G KUNG, HF TI NONHOMOLOGOUS CHROMATID EXCHANGE IN HEREDITARY AND SPORADIC RENAL-CELL CARCINOMAS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE ALLELE INACTIVATION; MITOTIC MECHANISM ID VONHIPPEL-LINDAU DISEASE; RETINOBLASTOMA; TRANSLOCATION; TUMORS; GENE; ABNORMALITIES; ONCOGENES; CANCER; TISSUE; REGION AB For the development of renal cell carcinomas, it has been suggested that a germ-line or somatic mutation occurs on one of the homologous chromosomes 3p, and subsequently the other 3p segment is lost. We have examined the karyotype and/or the allelic combination on chromosomes 3 and 5 by restriction fragment length polymorphism analysis in normal kidney and tumor samples from 28 renal cell carcinomas that developed in two patients with von Hippel-Lindau disease; we then compared the results to those of sporadic tumors. An unbalanced translocation between chromosome 3p and 5q or other chromosomes was found to be the most common aberration. We developed a model of nonhomologous chromatid exchange involving breakpoint clusters at chromosomes 3p13, 3p11.2, 5q22, and 8q11.2. Subsequent chromatid segregation may result in net loss of the 3p segment either (i) in one step or (ii) after a nondisjunctional loss of the derivative chromosome carrying the 3p segment. This general mechanism could also be implicated to explain genetic changes occurring in other types of solid tumors. C1 NCI,FREDERICK CANC RES FACIL,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701. RP KOVACS, G (reprint author), NCI,FREDERICK CANC RES FACIL,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,POB B,FREDERICK,MD 21701, USA. FU NCI NIH HHS [N01-CO-74102] NR 26 TC 78 Z9 78 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 1 BP 194 EP 198 DI 10.1073/pnas.88.1.194 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EQ544 UT WOS:A1991EQ54400042 PM 1986366 ER PT J AU SBURLATI, AR MANROW, RE BERGER, SL AF SBURLATI, AR MANROW, RE BERGER, SL TI PROTHYMOSIN-ALPHA ANTISENSE OLIGOMERS INHIBIT MYELOMA CELL-DIVISION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE OLIGODEOXYRIBONUCLEOTIDE; SYNCHRONIZED CELLS; HYBRID ARREST OF TRANSLATION; INTRACELLULAR OLIGONUCLEOTIDE STABILITY ID ALPHA-GENE; MESSENGER-RNA; EXPRESSION; PROTEIN; OLIGODEOXYRIBONUCLEOTIDES; PROLIFERATION; COMPLEMENTARY; TRANSLATION; POLYMERASE; TRANSPORT AB The function of prothymosin-alpha has been investigated by using four different antisense oligodeoxyribonucleotides directed at selected regions of its mRNA. In every case, when synchronized human myeloma cells were released from stationary phase by incubation in fresh medium containing antisense oligomers, cell division was prevented or inhibited; sense oligomers and random antisense oligomers had no effect. A detailed analysis of synchronized cell populations indicated that sense-treated and untreated cells divided almost-equal-to 17 hr after growth initiation, whereas cells incubated with antisense oligomer 183, a 16-mer targeted 5 bases downstream of the initiation codon, entered mitosis approximately one cell division late. The failure to divide correlated directly with a deficit in prothymosin-alpha and with the continued presence of intact intracellular antisense oligomers over a period of at least 24 hr. Because antisense oligomers had no effect either on the timing of the induction of prothymosin-alpha mRNA upon growth stimulation or on mRNA levels seen throughout the cell cycle, we concluded that antisense DNA caused specific hybrid arrest of translation. Our data suggest that prothymosin-alpha is required for cell division. However, there is no evidence that prothymosin-alpha directly regulates mitosis. C1 NCI,BIOCHEM LAB,GENES & GENE PROD PROD SECT,BLDG 10,ROOM B1B38,BETHESDA,MD 20892. NR 23 TC 154 Z9 157 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 1 BP 253 EP 257 DI 10.1073/pnas.88.1.253 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EQ544 UT WOS:A1991EQ54400054 PM 1986372 ER PT J AU SNEDEKER, SM BROWN, CF DIAGUSTINE, RP AF SNEDEKER, SM BROWN, CF DIAGUSTINE, RP TI EXPRESSION AND FUNCTIONAL-PROPERTIES OF TRANSFORMING GROWTH FACTOR-ALPHA AND EPIDERMAL GROWTH-FACTOR DURING MOUSE MAMMARY-GLAND DUCTAL MORPHOGENESIS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE MAMMARY EPITHELIUM; GROWTH FACTORS; IMMUNOHISTOCHEMISTRY; POLYMERASE CHAIN REACTION; TERMINAL END BUDS ID HUMAN-BREAST CANCER; MESSENGER RIBONUCLEIC-ACID; FACTOR-ALPHA CDNA; DEVELOPMENTAL EXPRESSION; EPITHELIAL-CELLS; FACTOR PRECURSOR; FACTOR RECEPTOR; HUMAN-TUMORS; TGF-ALPHA; ESTROGEN AB Primer-directed enzyme amplification was used to examine epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) mRNA transcripts in mammary glands of young virgin, mature virgin, midpregnant, and midlactating mice. Transcripts for both EGF and TGF-alpha mRNA were detected in virgin and pregnant mice, whereas transcripts for EGF mRNA but not TGF-alpha mRNA were expressed in 10-day lactating mice. TGF-alpha was localized in the epithelial cap-cell layer of the advancing terminal end bud and in the stromal fibroblasts at the base of the terminal end bud; EGF was localized in the inner layers of the terminal end bud and in ductal cells of mammary epithelium. Implantation of pellets containing EGF or TGF-alpha into the regressed mammary gland of ovariectomized mice stimulated the reappearance of end buds; contralateral glands implanted with pellets containing albumin or insulin were not affected. These results indicate that an EGF-receptor-mediated pathway remained intact in the mammary gland epithelium in the absence of ovarian steroids and that local availability of either EGF or TGF-alpha is sufficient to stimulate the pattern of normal ductal growth. The detection of EGF and TGF-alpha transcripts at different stages of mammary gland development and the different patterns of immunolocalization suggest that each polypeptide plays a different role in normal mammary gland morphogenesis. RP SNEDEKER, SM (reprint author), NIEHS,BIOCHEM RISK ANAL LAB,HORMONES & CANC WORKGRP,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 62 TC 194 Z9 194 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 1 BP 276 EP 280 DI 10.1073/pnas.88.1.276 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EQ544 UT WOS:A1991EQ54400059 PM 1986376 ER PT J AU AQUADRO, CF WEAVER, AL SCHAEFFER, SW ANDERSON, WW AF AQUADRO, CF WEAVER, AL SCHAEFFER, SW ANDERSON, WW TI MOLECULAR EVOLUTION OF INVERSIONS IN DROSOPHILA-PSEUDOOBSCURA - THE AMYLASE GENE REGION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE CHROMOSOMAL POLYMORPHISM; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; INVERSION PHYLOGENY; LINKED GENE COMPLEX ID EXPERIMENTAL POPULATIONS; MELANOGASTER; LOCUS; CLONING; ORGANIZATION; SEQUENCES; PATTERNS AB The amylase region of the third chromosome of Drosophila pseudoobscura has been cloned and localized to cytological band 73A. It is contained within a series of highly polymorphic inversions and serves as a convenient tool for a molecular evolutionary analysis of the inverted gene arrangements. Amylase in D. pseudoobscura is a family of three genes, and some chromosomes have deletions for one or two of them. Two overlapping clones covering 26 kilobases were isolated and used as probes to survey DNA restriction map polymorphism among 28 lines, representing five of the major inversion types found in natural populations, as well as single chromosomes from the closely related species Drosophila persimilis and Drosophila miranda. Restriction-site differences are considerably greater among the various gene arrangements than among chromosomes with the same gene arrangement. Clustering the restriction map haplotypes yielded a drendrogram concordant with the phylogeny generated independently from cytogenetic considerations. The inversion polymorphism is estimated to be about 2 million years old. C1 CORNELL UNIV,GENET & DEV SECT,ITHACA,NY 14853. UNIV GEORGIA,DEPT GENET,ATHENS,GA 30602. RP AQUADRO, CF (reprint author), NIEHS,GENET LAB,RES TRIANGLE PK,NC 27709, USA. RI Schaeffer, Stephen/B-1662-2010; OI Schaeffer, Stephen/0000-0003-2070-5342 FU NIGMS NIH HHS [GM36431, R01 GM036431] NR 35 TC 68 Z9 68 U1 0 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 1 BP 305 EP 309 DI 10.1073/pnas.88.1.305 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EQ544 UT WOS:A1991EQ54400065 PM 1702542 ER PT J AU OSHEA, JJ WEISSMAN, AM KENNEDY, ICS ORTALDO, JR AF OSHEA, JJ WEISSMAN, AM KENNEDY, ICS ORTALDO, JR TI ENGAGEMENT OF THE NATURAL-KILLER-CELL IGG FC RECEPTOR RESULTS IN TYROSINE PHOSPHORYLATION OF THE XI-CHAIN SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE TYROSINE KINASE; SIGNAL TRANSDUCTION; T-CELL ANTIGEN RECEPTOR; LARGE ANTIGRANULOCYTES LYMPHOCYTES ID ANTIGEN RECEPTOR; PROTEIN-KINASE; ZETA-CHAIN; GAMMA-RIII; ACTIVATION; COMPLEX; EXPRESSION; CD16; CD3; INTERLEUKIN-2 AB The zeta chain has emerged to be a key subunit of the T-cell antigen receptor with central roles not only in intracellular assembly of the multimeric receptor but also in mediating signal transduction events. This subunit is present in natural killer (NK) cells that lack the other subunits of the T-cell antigen receptor. In NK cells, the zeta chain appears to be associated with the NK Fc receptor [type 3 receptor for the Fc portion of IgG (Fc-gamma-RIII or CD16)] and may be necessary for efficient cell surface expression of this receptor complex. In T cells, the zeta chain is a prominent substrate that becomes phosphorylated on tyrosine residues after occupancy of the TCR; zeta chain phosphorylation was in fact the first evidence that the TCR was coupled to a protein-tyrosine kinase as well as to inositol phospholipid hydrolysis. To determine if Fc-gamma-RIII is coupled to a protein-tyrosine kinase in a manner analogous to the T-cell antigen receptor, we investigated ligand-dependent zeta-chain phosphorylation in NK cells. We observed that activation of NK cells with an anti-Fc-gamma-RIII monoclonal antibody induced tyrosine phosphorylation of the zeta chain whereas other activating stimuli, such as the combination of phorbol ester and ionomycin or a lymphokine, interleukin 2, did not result in phosphorylation of this protein. Perturbation of Fc-gamma-RIII by the more physiological stimulus, incubation of NK cells with antibody-coated target cells, also induced zeta-chain phosphorylation. Previous data have indicated that the NK-cell Fc-gamma-RIII is coupled to inositol phospholipid hydrolysis. This present finding that Fc-gamma-RIII is coupled to a protein-tyrosine kinase illustrates that there are significant similarities in the signaling pathways activated by Fc-gamma-RIII in NK cells and the T-cell antigen receptor in T cells; the zeta chain is a common element that may serve as a coupling protein for both of these receptors. C1 NCI, EXPTL IMMUNOL BRANCH, BETHESDA, MD 20892 USA. RP OSHEA, JJ (reprint author), NCI, FREDERICK CANC RES & DEV CTR, BIOLOGICAL RESPONSE MODIFIERS PROGRAM, EXPTL IMMUNOL LAB, FREDERICK, MD 21702 USA. NR 41 TC 129 Z9 129 U1 0 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 350 EP 354 DI 10.1073/pnas.88.2.350 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100008 PM 1703295 ER PT J AU DAVIS, MD KAUFMAN, S MILSTIEN, S AF DAVIS, MD KAUFMAN, S MILSTIEN, S TI CONVERSION OF 6-SUBSTITUTED TETRAHYDROPTERINS TO 7-ISOMERS VIA PHENYLALANINE HYDROXYLASE-GENERATED INTERMEDIATES SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE HYPERPHENYLALANINEMIA; TETRAHYDROBIOPTERIN ID DIHYDROPTERIDINE-REDUCTASE; ATYPICAL PHENYLKETONURIA; RAT-LIVER; SYNTHETASE DEFICIENCY; TYROSINE-HYDROXYLASE; PYRIMIDINE COFACTORS; HYPERPHENYLALANINEMIA; TETRAHYDROBIOPTERIN; PURIFICATION; BIOPTERIN AB This isomer had not been found previously in humans, and although its origin was not established, preliminary evidence suggested that it might be produced from 6-biopterin. We have now found that 7-biopterin can be formed in vitro from (6R)-tetrahydrobiopterin during the hydroxylation of phenylalanine catalyzed by phenylalanine hydroxylase [L-phenylalanine, tetrahydrobiopterin:oxygen oxidoreductase (4-hydroxylating), EC 1.14.16.1]. The resulting 7-biopterin was unequivocally identified by the following criteria: preparative isolation and conversion to 7-hydroxymethyipterin following periodate oxidation and borohydride reduction, quantitative conversion to pterin-7-carboxylic acid after oxidation with permanganate, and liquid chromatography/thermospray mass spectrometry. Addition of 4a-carbinolamine dehydratase, an enzyme involved in the regeneration of tetrahydrobiopterin from the pterin carbinolamine intermediate (also called 4a-hydroxytetrahydrobiopterin) formed in the phenylalanine hydroxylase reaction, greatly decreased the amount of the 7-biopterin formed. This result implies that the in vitro formation of 7-biopterin occurs via the nonenzymatic rearrangement of the unstable substrate of the dehydratase, 4a-hydroxytetrahydrobiopterin, and suggests that this new variant of hyperphenylalaninemia may be caused by a lack of 4a-carbinolamine dehydratase activity. A mechanism for the rearrangement is proposed that predicts that other 6-substituted tetrahydropterin substrates of the aromatic amino acid hydroxylases could also give rise to rearranged products from an opening of the pyrazine ring of the corresponding 4a-hydroxytetrahydropterin intermediate. RP DAVIS, MD (reprint author), NIMH,NEUROCHEM LAB,BETHESDA,MD 20892, USA. NR 41 TC 61 Z9 61 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 385 EP 389 DI 10.1073/pnas.88.2.385 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100015 PM 1988938 ER PT J AU BRENNER, M TOMIZAWA, JI AF BRENNER, M TOMIZAWA, JI TI QUANTITATION OF COLE1-ENCODED REPLICATION ELEMENTS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE PLASMID COPY NUMBER; RNA-I; RNA-II; ROM PROTEIN ID COLE1 PLASMID REPLICATION; RNA-I; PRIMER TRANSCRIPT; GENE-PRODUCT; COPY NUMBER; BINDING; PROTEIN; DNA; INITIATION; INHIBITION AB Replication of the Escherichia coli plasmid ColEl initiates from an RNA primer. This primer is formed by a ColEl RNA II molecule that remains hybridized to its DNA template in the origin region after transcription. Continued hybridization is inhibited by prior binding to RNA II of another ColEl transcript, RNA I; and this interaction is regulated by the plasmid-encoded Rom protein. To understand the quantitative aspects of regulation of ColE1 synthesis, we have measured the levels of RNA I, RNA II, and Rom protein in vivo, as well as the half-lives of the RNAs. The intracellular concentrations of RNA I, RNA II, and Rom protein were found to be about 1-mu-M, 7 nM, and 1-muM, respectively; and the RNAs had half-lives of about 2 min. A simple model derived from these results indicates that the plasmid copy number is little affected by the rate of RNA II synthesis but is strongly dependent on that of RNA I. C1 NIDDKD,MOLECUL BIOL LAB,BETHESDA,MD 20892. NR 25 TC 50 Z9 50 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 405 EP 409 DI 10.1073/pnas.88.2.405 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100019 PM 1703297 ER PT J AU RUBIN, JS CHAN, AML BOTTARO, DP BURGESS, WH TAYLOR, WG CECH, AC HIRSCHFIELD, DW WONG, J MIKI, T FINCH, PW AARONSON, SA AF RUBIN, JS CHAN, AML BOTTARO, DP BURGESS, WH TAYLOR, WG CECH, AC HIRSCHFIELD, DW WONG, J MIKI, T FINCH, PW AARONSON, SA TI A BROAD-SPECTRUM HUMAN LUNG FIBROBLAST-DERIVED MITOGEN IS A VARIANT OF HEPATOCYTE GROWTH-FACTOR SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE HEPARIN-BINDING GROWTH FACTOR; PLASMINOGEN; EPITHELIAL CELLS; ENDOTHELIAL CELLS; MELANOCYTES ID MOLECULAR-CLONING; TYROSINE KINASES; PURIFICATION; CELLS; EXPRESSION; CANCER; CDNA; INSERTS AB A heparin-binding mitogen was isolated from conditioned medium of human embryonic lung fibroblasts. It exhibited broad target-cell specificity whose pattern was distinct from that of any known growth factor. It rapidly stimulated tyrosine phosphorylation of a 145-kDa protein in responsive cells, suggesting that its signaling pathways involved activation of a tyrosine kinase. Purification identified a major polypeptide with an apparent molecular mass of 87 kDa under reducing conditions. Partial amino acid sequence analysis and cDNA cloning revealed that it was a variant of hepatocyte growth factor, a mitogen thought to be specific for hepatic cells and structurally related to plasminogen. Recombinant expression of the cDNA in COS-1 cells established that it encoded the purified growth factor. Its site of synthesis and spectrum of targets imply that this growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. C1 NCI,CELLULAR & MOLEC BIOL LAB,BLDG 37,ROOM 1E24,BETHESDA,MD 20892. AMER RED CROSS,JEROME H HOLLAND LAB BIOMED SCI,MOLEC BIOL LAB,ROCKVILLE,MD 20855. RI Bottaro, Donald/F-8550-2010 OI Bottaro, Donald/0000-0002-5057-5334 NR 40 TC 550 Z9 557 U1 0 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 415 EP 419 DI 10.1073/pnas.88.2.415 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100021 PM 1824873 ER PT J AU CHARIFSON, PS DARDEN, T TULINSKY, A HUGHEY, JL HISKEY, RG PEDERSEN, LG AF CHARIFSON, PS DARDEN, T TULINSKY, A HUGHEY, JL HISKEY, RG PEDERSEN, LG TI SOLUTION CONFORMATIONS OF THE GAMMA-CARBOXYGLUTAMIC ACID DOMAIN OF BOVINE PROTHROMBIN FRAGMENT-1, RESIDUES 1-65 SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE MOLECULAR DYNAMICS; CRYSTAL STRUCTURE; PROLINE ISOMERIZATION ID NUCLEAR MAGNETIC-RESONANCE; CIS-TRANS ISOMERIZATION; VITAMIN-K; PROLINE ISOMERIZATION; RIBONUCLEASE-A; PROTEIN; IONS; RESOLUTION; SEQUENCE; PEPTIDES AB Molecular dynamics simulations have been performed (AMBER version 3.1) on solvated residues 1-65 of bovine prothrombin fragment 1 (BF1) by using the 2.8-angstrom resolution crystallographic coordinates as the starting conformation for understanding calcium ion-induced conformational changes that precede experimentally observable phospholipid binding. Simulations were performed on the non-metal-bound crystal structure, the form resulting from addition of eight calcium ions to the 1-65 region of the crystal structure, the form resulting from removal of calcium ions after 107 ps and continuing the simulation, and an isolated hexapeptide loop (residues 18-23). In all cases, the 100-ps time scale seemed adequate to sample an ensemble of solution conforms within a particular region of conformation space. The non-metal-containing BF1 did not unfold appreciably during a 106-ps simulation starting from the crystallographic geometry. The calcium ion-containing structure (Ca-BF1) underwent an interesting conformational reorganization during its evolution from the crystal structure: during the time course of a 107-ps simulation, Ca-BF1 experienced a trans --> cis isomerization of the gamma-carboxyglutamic acid-21 (Gla-21)-Pro-22 peptide bond. Removal of the calcium ions from this structure followed by 114 ps of additional molecular dynamics showed significant unfolding relative to the final 20-ps average structure of the 107-ps simulation; however, the Gla-21-Pro-22 peptide bond remained cis. A 265-ps simulation on the termini-protected hexapeptide loop (Cys-18 to Cys-23) containing two calcium ions also did not undergo a trans --> cis isomerization. It is believed that the necessary activation energy for the transitional event observed in the Ca-BF1 simulation was largely supplied by global conformational events with a possible assist from relief of intermolecular crystal packing forces. The presence of a Gla preceding Pro-22, the inclusion of Pro-22 in a highly strained loop structure, and the formation of two long-lived salt bridges prior to isomerization may all contribute to this finding. C1 UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599. NIEHS,RES TRIANGLE PK,NC 27709. MICHIGAN STATE UNIV,DEPT CHEM,E LANSING,MI 48824. RI Pedersen, Lee/E-3405-2013 OI Pedersen, Lee/0000-0003-1262-9861 FU NHLBI NIH HHS [HL-23609, HL-20161, HL-27995] NR 37 TC 10 Z9 10 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 424 EP 428 DI 10.1073/pnas.88.2.424 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100023 PM 1988943 ER PT J AU WU, XM GUTFREUND, H LAKATOS, S CHOCK, PB AF WU, XM GUTFREUND, H LAKATOS, S CHOCK, PB TI SUBSTRATE CHANNELING IN GLYCOLYSIS - A PHANTOM PHENOMENON SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE MECHANISM; METABOLITE TRANSFER; GLYCOLYTIC ENZYMES; DISPLACEMENT REACTIONS ID NICOTINAMIDE ADENINE-DINUCLEOTIDE; ENZYME-ENZYME COMPLEXES; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; PHOSPHOGLYCERATE KINASE; LACTATE-DEHYDROGENASE; MECHANISM; ALDOLASE; NADH AB It has been proposed that glycolytic enzymes form multienzyme complexes for direct transfer of metabolites from the producing enzyme to the utilizing one. Reexamination of the supporting evidence, which involves the transfer of NADH between its complexes with glycerol-3-phosphate dehydrogenase (alpha-glycerol phosphate dehydrogenase, GPDH; EC 1.1.1.8) and with L-lactate dehydrogenase (LDH;EC 1.1.1.27), has shown that the supporting evidence is based on misinterpretation of the kinetics of ligand exchange [Chock, P.B. & Gutfreund, H. (1988) Proc. Natl. Acad. Sci. USA 85, 8870-8874]. Srivastava et al. [Srivastava, D.K., Smolen, P., Betts, G.F., Fukushima, T., Spivey, H. O. & Bernhard, S.A. (1989) Proc. Natl. Acad. Sci. USA 86,6464-6468] have responded with a revision of their own and criticism of our data. To clarify this problem, we have carried out detailed kinetic studies on NADH binding to GPDH and LDH and on the displacement of enzyme-bound NADH by LDH or GPDH. The experiments were conducted at 10-degrees-C in 50 mM Hepes, pH 7.5/100 mN KCI/1 mM EDTA/1 mM 2-mercaptoethanol, using rabbit muscle GPDH and LDH. The results show that the kinetic patterns exhibited by the displacement of NADH-bound enzyme by either GPDH or LDH are consistent with a dissociative mechanism but not with a direct transfer mechanism. Theoretical analysis shows that a combined disociative and direct transfer mechanism can explain the transient kinetic data reported by Srivastava et al. if, and only if, a majority (almost-equal-to 90%) of the enzyme present in lower concentration exists as a complex with the second enzyme. However, data from tracer and traditional sedimentation equilibrium and from gel filtration experiments show that LDH and GPDH do not form complexes in the presence of saturating NADH concentration when the enzyme concentrations are ranged between 4 and 50 mu-M, a concentration equal to or greater than that used by Srivastava et al. Our results demonstrate that GPDH and LDH do not form multienzyme complex and the transfer of NADH between these enzymes proceeds via a dissociative mechanism. C1 NIDDKD,BIOCHEM PHARMACOL LAB,BETHESDA,MD 20892. UNIV BRISTOL,DEPT BIOCHEM,BRISTOL BS8 1TH,AVON,ENGLAND. RP WU, XM (reprint author), NHLBI,BIOCHEM LAB,BETHESDA,MD 20892, USA. NR 16 TC 50 Z9 50 U1 1 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 497 EP 501 DI 10.1073/pnas.88.2.497 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100038 PM 1988948 ER PT J AU BALDWIN, ET WEBER, IT STCHARLES, R XUAN, JC APPELLA, E YAMADA, M MATSUSHIMA, K EDWARDS, BFP CLORE, GM GRONENBORN, AM WLODAWER, A AF BALDWIN, ET WEBER, IT STCHARLES, R XUAN, JC APPELLA, E YAMADA, M MATSUSHIMA, K EDWARDS, BFP CLORE, GM GRONENBORN, AM WLODAWER, A TI CRYSTAL-STRUCTURE OF INTERLEUKIN-8 - SYMBIOSIS OF NMR AND CRYSTALLOGRAPHY SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE CHEMOTAXIS; MOLECULAR REPLACEMENT; RECEPTOR BINDING; GROWTH FACTORS ID NUCLEAR MAGNETIC-RESONANCE; X-RAY-DIFFRACTION; AMYLASE INHIBITOR TENDAMISTAT; NEUTROPHIL CHEMOTACTIC FACTOR; LEAST-SQUARES REFINEMENT; MACROMOLECULAR STRUCTURES; PLATELET FACTOR-4; PROTEIN; SPECTROSCOPY; PURIFICATION AB The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 angstrom x-ray data to an R factor of 0.187 (0.217 at 1.6 angstrom). A comparison indicates some potential differences between the structure in solution and in the crystalline state. Our analysis also predicts that residues 4 through 9 on the amino terminus and the beta-bend, which includes His-33, may be important for receptor binding. C1 NCI,CELL BIOL LAB,BETHESDA,MD 20892. DAINIPPON PHARMACEUT CO LTD,RES LAB,SUITA,OSAKA 564,JAPAN. NCI,BIOL RESPONSE MODIFIERS PROGRAM,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21702. WAYNE STATE UNIV,DEPT PHYSIOL,DETROIT,MI 48201. WAYNE STATE UNIV,SCH MED,DEPT BIOCHEM,DETROIT,MI 48201. NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. RP BALDWIN, ET (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,CRYSTALLOG LAB,FREDERICK,MD 21702, USA. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 FU NCI NIH HHS [N01-CO-74101] NR 36 TC 309 Z9 312 U1 0 U2 8 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 502 EP 506 DI 10.1073/pnas.88.2.502 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100039 PM 1988949 ER PT J AU GIRARD, M KIENY, MP PINTER, A BARRESINOUSSI, F NARA, P KOLBE, H KUSUMI, K CHAPUT, A REINHART, T MUCHMORE, E RONCO, J KACZOREK, M GOMARD, E GLUCKMAN, JC FULTZ, PN AF GIRARD, M KIENY, MP PINTER, A BARRESINOUSSI, F NARA, P KOLBE, H KUSUMI, K CHAPUT, A REINHART, T MUCHMORE, E RONCO, J KACZOREK, M GOMARD, E GLUCKMAN, JC FULTZ, PN TI IMMUNIZATION OF CHIMPANZEES CONFERS PROTECTION AGAINST CHALLENGE WITH HUMAN-IMMUNODEFICIENCY-VIRUS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE VACCINE; AIDS; NEUTRALIZING ANTIBODIES ID ESCHERICHIA-COLI; CHAIN-REACTION; HIV INFECTION; ENVELOPE; RECOMBINANT; ANTIBODIES; VACCINE; REPLICATION; SEQUENCE; INVITRO AB Sustained high titers of neutralizing antibodies were elicited in three chimpanzees after sequential injections of different human immunodeficiency virus 1 (HIV-1) antigen preparations derived from the HIV-1 BRU strain that included whole inactivated virus or purified recombinant proteins and then synthetic peptides identical to the major HIV-1 neutralizing epitope V3. The animals were challenged i.v. with 40 chimpanzee infectious doses (equivalent to 100 tissue culture 50% infectious doses) of a stock of HIV-1 IIIB isolate. After 6 mo of follow-up, all three animals appeared uninfected by serologic and virologic criteria, including polymerase chain reaction analysis and failure to isolate virus from peripheral blood lymphocytes, bone marrow, and lymph node tissue. Of two chimpanzees monitored for 1 yr, virus was isolated initially from one animal at 32 weeks, but the second chimpanzee was virus negative by all assays through 12 mo; the third animal has remained virus negative through 9 mo of follow-up. These results indicate that it is possible to elicit protection against, or significantly delay infection of, HIV-1 by immunization, thus laying the foundation for development of an HIV-1 vaccine. C1 TRANSGENE SA,F-67082 STRASBOURG,FRANCE. PUBL HLTH RES INST CITY NEW YORK INC,NEW YORK,NY 10016. NCI,FREDERICK,MD 21701. HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. STANFORD UNIV,MED CTR,STANFORD,CA 94305. NYU MED CTR,EXPTL MED & SURG PRIMATES LAB,NEW YORK,NY 10016. PASTEUR MERIEUX SERUMS & VACCINS,F-27101 VAL DE REUIL,FRANCE. HOP COCHIN,INSERM,U152,F-75674 PARIS 14,FRANCE. EMORY UNIV,YERKES PRIMATE RES CTR,ATLANTA,GA 30322. HOP LA PITIE SALPETRIERE,F-75013 PARIS,FRANCE. RP GIRARD, M (reprint author), INST PASTEUR,25 RUE DR ROUX,F-75724 PARIS 15,FRANCE. RI Barre-Sinoussi, Francoise/G-8355-2011; Kusumi, Kenro/J-2626-2012 OI Kusumi, Kenro/0000-0002-1458-4540 FU NIAID NIH HHS [AI85007] NR 34 TC 374 Z9 375 U1 1 U2 10 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 542 EP 546 DI 10.1073/pnas.88.2.542 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100047 PM 1988952 ER PT J AU MICKISCH, GH MERLINO, GT GALSKI, H GOTTESMAN, MM PASTAN, I AF MICKISCH, GH MERLINO, GT GALSKI, H GOTTESMAN, MM PASTAN, I TI TRANSGENIC MICE THAT EXPRESS THE HUMAN MULTIDRUG-RESISTANCE GENE IN BONE-MARROW ENABLE A RAPID IDENTIFICATION OF AGENTS THAT REVERSE DRUG-RESISTANCE SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE CHEMOSENSITIZERS; DAUNOMYCIN; TAXOL/(R)-VERAPAMIL; CHEMOTHERAPY ID P-GLYCOPROTEIN; CELLS; MDR1; VERAPAMIL; CDNA; VINBLASTINE; TRANSPORT; VESICLES AB The development of preclinical models for the rapid testing of agents that circumvent multidrug resistance in cancer is a high priority of research on drug resistance. A common form of multidrug resistance in human cancer results from expression of the MDR1 gene, which encodes a M(r)170,000 glycoprotein that functions as a plasma membrane energy-dependent multidrug efflux pump. We have engineered transgenic mice that express this multidrug transporter in their bone marrow and demonstrated that these animals are resistant to leukopenia by a panel of anticancer drugs including anthracyclines, vinca alkaloids, etoposide, taxol, and actinomycin D. Differential leukocyte counts indicate that both neutrophils and lymphocytes are protected. Drugs such as cisplatin, methotrexate, and 5-fluorouracil, which are not handled by the multidrug transporter, produce bone marrow suppression in both normal and transgenic mice. The resistance conferred by the MDR1 gene can be circumvented in a dose-dependent manner by simultaneous administration of agents previously shown to be inhibitors of the multidrug transporter in vitro, including verapamil isomers, quinidine, and quinine. Verapamil and quinine, both at levels suitable for human trials that produced only partial sensitization of the MDR1-transgenic mice, were fully sensitizing when used in combination. We conclude that MDR1-transgenic mice provide a rapid and reliable system to determine the bioactivity of agents that reverse multidrug resistance in animals. C1 NCI,MOLEC BIOL LAB,BLDG 37,ROOM 4E16,BETHESDA,MD 20892. NCI,CELL BIOL LAB,BETHESDA,MD 20892. NCI,DIV CANC BIOL DIAGN & CTR,BETHESDA,MD 20892. NR 29 TC 151 Z9 156 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 547 EP 551 DI 10.1073/pnas.88.2.547 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100048 PM 1671173 ER PT J AU ROBEY, E RAMSDELL, F ELLIOTT, J RAULET, D KIOUSSIS, D AXEL, R FOWLKES, BJ AF ROBEY, E RAMSDELL, F ELLIOTT, J RAULET, D KIOUSSIS, D AXEL, R FOWLKES, BJ TI EXPRESSION OF CD4 IN TRANSGENIC MICE ALTERS THE SPECIFICITY OF CD8 CELLS FOR ALLOGENEIC MAJOR HISTOCOMPATIBILITY COMPLEX SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE T-CELL RECEPTOR; MAJOR HISTOCOMPATIBILITY COMPLEX CLASS SPECIFICITY; ALLOREACTIVITY; ACCESSORY MOLECULES ID MHC CLASS-I; T-CELL; LYMPHOCYTES-T; ANTIGEN; RECEPTOR; GENE; SUBSETS; CLONE; ALLOANTIGENS; POPULATIONS AB We have generated a transgenic mouse line in which a CD4 transgene is expressed on a significant fraction of the mature CD8+ lymphocytes but is not expressed in the thymus. This provides an opportunity to examine the functional consequences of CD4 expression in a population of class I-selected CD8+ lymphocytes. CD8+ lymphocytes expressing the CD4 transgene proliferate in response to allogeneic class I and class II major histocompatibility complex, whereas CD8+ cells from control animals proliferate only to allogeneic class I gene products. These observations suggest that the ability of a T-cell population to react with class II allogeneic major histocompatibility complex is determined by the presence of CD4. C1 COLUMBIA UNIV,HOWARD HUGHES MED INST,NEW YORK,NY 10032. NIAID,CELLULAR & MOLEC IMMUNOL LAB,BETHESDA,MD 20892. NATL INST MED RES,GENE STRUCT & EXPRESS LAB,LONDON NW7 1AA,ENGLAND. MIT,CTR CANC RES,CAMBRIDGE,MA 02139. RP ROBEY, E (reprint author), COLUMBIA UNIV,DEPT BIOCHEM & MOLEC BIOPHYS,NEW YORK,NY 10032, USA. NR 31 TC 29 Z9 29 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN PY 1991 VL 88 IS 2 BP 608 EP 612 DI 10.1073/pnas.88.2.608 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ET521 UT WOS:A1991ET52100061 PM 1824875 ER PT J AU NOJI, S TANIGUCHI, S KON, H AF NOJI, S TANIGUCHI, S KON, H TI AN EPR STUDY ON ERYTHROCYTE DEFORMABILITY SO PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY LA English DT Review ID ELECTRON-PARAMAGNETIC-RES; RESONANCE SPECTRAL CHANGE; SHEAR-FLOW; SPIN-LABEL; ORIENTATION CHARACTERISTICS; CELL DEFORMABILITY; MEMBRANE; DEFORMATION; SHAPE; ATP C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. RP NOJI, S (reprint author), OKAYAMA UNIV,SCH DENT,DEPT BIOCHEM,2-5-1 SHIKATA CHO,OKAYAMA 700,JAPAN. NR 61 TC 10 Z9 10 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0079-6107 J9 PROG BIOPHYS MOL BIO JI Prog. Biophys. Mol. Biol. PY 1991 VL 55 IS 2 BP 85 EP 105 DI 10.1016/0079-6107(91)90002-A PG 21 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FW735 UT WOS:A1991FW73500002 PM 1651527 ER PT J AU MARGOLIS, FL VERHAAGEN, J BIFFO, S HUANG, FL GRILLO, M AF MARGOLIS, FL VERHAAGEN, J BIFFO, S HUANG, FL GRILLO, M TI REGULATION OF GENE-EXPRESSION IN THE OLFACTORY NEUROEPITHELIUM - A NEUROGENETIC MATRIX SO PROGRESS IN BRAIN RESEARCH LA English DT Review ID PROTEIN-KINASE-C; CALMODULIN-BINDING-PROTEIN; GROWTH-RELATED PROTEIN; ADULT-RAT BRAIN; RECEPTOR NEURONS; NERVE GROWTH; TYROSINE-HYDROXYLASE; TRANSPORTED PROTEINS; MONOCLONAL-ANTIBODY; AXONAL REGENERATION C1 RUDOLF MAGNUS INST PHARMACOL, DIV MOLEC BIOL, UTRECHT, NETHERLANDS. RUDOLF MAGNUS INST MOLEC BIOL & MED BIOTECHNOL, UTRECHT, NETHERLANDS. NICHHD, BETHESDA, MD 20892 USA. UNIV TURIN, DIPARTMENTO BIOL ANIM, I-10124 TURIN, ITALY. RP ROCHE INST MOLEC BIOL, DEPT NEUROSCI, NUTLEY, NJ 07110 USA. NR 87 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0079-6123 J9 PROG BRAIN RES JI Prog. Brain Res. PY 1991 VL 89 BP 97 EP 122 PG 26 WC Neurosciences SC Neurosciences & Neurology GA GZ403 UT WOS:A1991GZ40300009 ER PT J AU HUANG, KP HUANG, FL MAHONEY, CW CHEN, KH AF HUANG, KP HUANG, FL MAHONEY, CW CHEN, KH TI PROTEIN-KINASE-C SUBTYPES AND THEIR RESPECTIVE ROLES SO PROGRESS IN BRAIN RESEARCH LA English DT Review ID CEREBELLAR GRANULE CELLS; RAT-BRAIN; PHORBOL ESTERS; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; IMMUNOCYTOCHEMICAL LOCALIZATION; ISOZYMES; ACTIVATION; PHOSPHORYLATION; TRANSMITTER; EXPRESSION C1 NICHHD, METAB REGULAT SECT, BETHESDA, MD 20892 USA. NICHHD, ENDOCRINOL & REPROD RES BRANCH, BETHESDA, MD 20892 USA. NR 48 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0079-6123 J9 PROG BRAIN RES JI Prog. Brain Res. PY 1991 VL 89 BP 143 EP 155 PG 13 WC Neurosciences SC Neurosciences & Neurology GA GZ403 UT WOS:A1991GZ40300011 ER PT J AU SUTIN, EL JACOBOWITZ, DM AF SUTIN, EL JACOBOWITZ, DM TI NEUROCHEMICALS IN THE DORSAL PONTINE TEGMENTUM SO PROGRESS IN BRAIN RESEARCH LA English DT Review DE LOCUS-CERULEUS; DORSAL PONTINE TEGMENTUM; IMMUNOFLUORESCENCE MICROSCOPY; LATERAL DORSAL TEGMENTAL NUCLEUS; TYROSINE HYDROXYLASE ID CENTRAL NERVOUS-SYSTEM; SUBSTANCE-P-LIKE; RAT-BRAIN; LOCUS COERULEUS; IMMUNOREACTIVE NEURONS; TYROSINE-HYDROXYLASE; NEUROPEPTIDE-Y; SPINAL-CORD; ULTRASTRUCTURAL-LOCALIZATION; PANCREATIC-POLYPEPTIDE AB Detailed maps of neurochemicals in the locus coeruleus and adjacent dorsal tegmental areas are discussed in this chapter. The locus coeruleus appears to be one of the most complex brain regions with six neurochemicals (acetylcholinesterase, tyrosine hydroxylase, galanin, neuropeptide Y, neurotensin, and vasoactive intestinal protein) contained within the cell bodies. C1 NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NR 53 TC 22 Z9 22 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0079-6123 J9 PROG BRAIN RES JI Prog. Brain Res. PY 1991 VL 88 BP 3 EP 14 PG 12 WC Neurosciences SC Neurosciences & Neurology GA GX165 UT WOS:A1991GX16500001 PM 1726029 ER PT J AU MURRAY, EA AF MURRAY, EA TI CONTRIBUTIONS OF THE AMYGDALAR COMPLEX TO BEHAVIOR IN MACAQUE MONKEYS SO PROGRESS IN BRAIN RESEARCH LA English DT Review ID STIMULUS-REWARD ASSOCIATIONS; VISUAL CORTICAL AREAS; RHESUS-MONKEY; SUBSTANTIA INNOMINATA; LATERAL HYPOTHALAMUS; AFFERENT CONNECTIONS; MACACA-FASCICULARIS; BASAL FOREBRAIN; THALAMIC NUCLEUS; MEMORY RP NIMH, NEUROPSYCHOL LAB, BETHESDA, MD 20892 USA. NR 74 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0079-6123 J9 PROG BRAIN RES JI Prog. Brain Res. PY 1991 VL 87 BP 167 EP 180 PG 14 WC Neurosciences SC Neurosciences & Neurology GA HB641 UT WOS:A1991HB64100008 ER PT J AU DIWAN, BA WARD, JM RICE, JM AF DIWAN, BA WARD, JM RICE, JM TI MODIFICATION OF LIVER-TUMOR DEVELOPMENT IN RODENTS SO PROGRESS IN EXPERIMENTAL TUMOR RESEARCH LA English DT Article ID 2-ACETYLAMINOFLUORENE-INDUCED HEPATIC TUMORIGENESIS; GLUTAMYLTRANSPEPTIDASE-POSITIVE FOCI; CARCINOGEN-TREATED RATS; ENZYME-ALTERED ISLANDS; METHYL-N-NITROSOUREA; DIETARY OROTIC-ACID; PRENEOPLASTIC FOCI; PROMOTING ACTIVITIES; FEMALE RATS; MOUSE HEPATOCARCINOGENESIS C1 NCI,FREDERICK CANC RES FACIL,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21701. PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD. FU NCI NIH HHS [N01-CO-74102] NR 154 TC 7 Z9 7 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0079-6263 J9 PROG EXP TUMOR RES JI Prog. Exp. Tumor Res. PY 1991 VL 33 BP 76 EP 107 PG 32 WC Oncology SC Oncology GA EZ865 UT WOS:A1991EZ86500005 PM 2028027 ER PT J AU BREWER, HB RADER, DJ AF BREWER, HB RADER, DJ TI HDL - STRUCTURE, FUNCTION AND METABOLISM SO PROGRESS IN LIPID RESEARCH LA English DT Article ID APOLIPOPROTEIN-A-I; HIGH-DENSITY LIPOPROTEIN; CORONARY-ARTERY DISEASE; TANGIER DISEASE; HEPATIC LIPASE; C-III; DEFICIENCY; PLASMA; CONVERSION; INVITRO RP BREWER, HB (reprint author), NHLBI,MOLEC DIS BRANCH,BETHESDA,MD 20892, USA. NR 24 TC 20 Z9 20 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0163-7827 J9 PROG LIPID RES JI Prog. Lipid. Res. PY 1991 VL 30 IS 2-3 BP 139 EP 144 PG 6 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA GY734 UT WOS:A1991GY73400003 PM 1823933 ER PT J AU GLOWA, JR BACHER, JD HERKENHAM, M GOLD, PW AF GLOWA, JR BACHER, JD HERKENHAM, M GOLD, PW TI SELECTIVE ANOREXIGENIC EFFECTS OF CORTICOTROPIN RELEASING HORMONE IN THE RHESUS-MONKEY SO PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY LA English DT Article DE ANOREXIGENIC EFFECTS; ANXIETY; CORTICOTROPIN RELEASING HORMONE; DEPRESSION; SCHEDULE-CONTROLLED BEHAVIOR; RHESUS MONKEYS ID FACTOR CRF; SQUIRREL-MONKEYS; BEHAVIOR; FOOD; RATS AB 1. Rhesus monkeys were equipped with a novel intracerebroventricular (i.c.v.) cannula system and trained to respond under operant schedules of food presentation or termination of stimuli associated with the delivery of shock (escape). 2. CRH decreased food-maintained behavior in a dose-related manner over the range of (0.3-10-mu-g/kg) but did not affect escape responding, demonstrating a selective effect on food-maintained responding. 3. This selective effect was related to the tendency for responding to stop after delivery of a food pellet when higher doses of CRH were given, consistent with the notion that a conditioned aversion to food was established in the presence of CRH. 4. This may suggest that in hyperaroused clinical states such as depression and anorexia nervosa, focus is shifted away from appetitive tasks as a result of increased levels of CRH. C1 NIH,NCRR,VET RESOURCES PROGRAM,BETHESDA,MD 20892. RP GLOWA, JR (reprint author), NIMH,CLIN NEUROENDOCRINOL BRANCH,BIOPSYCHOL UNIT,BLDG 14D,RM 311,BETHESDA,MD 20892, USA. OI Herkenham, Miles/0000-0003-2228-4238 NR 25 TC 8 Z9 8 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0278-5846 J9 PROG NEURO-PSYCHOPH JI Prog. Neuro-Psychopharmacol. Biol. Psychiatry PY 1991 VL 15 IS 3 BP 379 EP 391 DI 10.1016/0278-5846(91)90069-D PG 13 WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA FH474 UT WOS:A1991FH47400007 PM 1862219 ER PT J AU FAGARASAN, MO ARORA, PK AXELROD, J AF FAGARASAN, MO ARORA, PK AXELROD, J TI INTERLEUKIN-1 POTENTIATION OF BETA-ENDORPHIN SECRETION AND THE DYNAMICS OF INTERLEUKIN-1 INTERNALIZATION IN PITUITARY-CELLS SO PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY LA English DT Article DE BETA-ENDORPHIN SECRETION; INTERLEUKIN-1; INTERLEUKIN-1 RECEPTORS INTERNALIZATION; PROTEIN KINASE-C ID ATT-20; LINE AB 1. This study demonstrates that human recombinant interleukin-1 (IL-1) stimulates beta-endorphin release and potentiates the secretion of beta-endorphin in both a mouse anterior pituitary cell line AtT-20 and rat pituitary cell cultures. 2. In pituitary cell cultures, prolonged treatment with phorbol ester had no effect on IL-1-induced beta-endorphin release, but abolished the potentiating effects of IL-1 on vasopressin-induced beta-endorphin secretion. 3. The enhancement of CRF-stimulated beta-endorphin release by IL-1 was also reduced in normal pituitary cell cultures following depletion of protein kinase C. 4. The late IL-1-induced secretion of beta-endorphin does not require the continuous presence of the cytokine. 5. Incubation of monolayers with I-125-IL-1-alpha (10(-9) M) at 8-degrees-C and then at 37-degrees-C for various times revealed that IL-1-alpha was internalized. There was a progressive increase in the ratio of cytoplasmic to cell-surface-associated I-125-IL-1-alpha. 6. These results indicate that the IL-1-induced beta-endorphin release and its potentiation of beta-endorphin secretion involves internalization of this cytokine, perhaps via cell surface IL-1 receptors. C1 NIMH,NEUROSCI LAB,BETHESDA,MD 20892. NR 9 TC 9 Z9 9 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0278-5846 J9 PROG NEURO-PSYCHOPH JI Prog. Neuro-Psychopharmacol. Biol. Psychiatry PY 1991 VL 15 IS 4 BP 551 EP 560 DI 10.1016/0278-5846(91)90030-5 PG 10 WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GC159 UT WOS:A1991GC15900009 PM 1749831 ER PT J AU RAFFAELE, KC BERARDI, A MORRIS, PP ASTHANA, S HAXBY, JV SCHAPIRO, MB RAPOPORT, SI SONCRANT, TT AF RAFFAELE, KC BERARDI, A MORRIS, PP ASTHANA, S HAXBY, JV SCHAPIRO, MB RAPOPORT, SI SONCRANT, TT TI EFFECTS OF ACUTE INFUSION OF THE MUSCARINIC CHOLINERGIC AGONIST ARECOLINE ON VERBAL MEMORY AND VISUOSPATIAL FUNCTION IN DEMENTIA OF THE ALZHEIMER TYPE SO PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY LA English DT Review DE ALZHEIMERS DISEASE; ARECOLINE; BRAIN; CHOLINERGIC FUNCTION; MEMORY ID DISEASE; PHYSOSTIGMINE; SYSTEM; THERAPY; TRIAL AB 1. Treatment of patients with dementia of the Alzheimer type (DAT) with arecoline, a muscarinic cholinergic receptor agonist, reportedly improves performance on a picture recognition memory task, but not on other memory measures. To examine further possible performance improvements following arecoline treatment, patients with DAT were treated with a 30 min intravenous infusion of arecoline (5 mg). 2. Psychometric testing was dons at five time points (two before and three following the infusion). Patients were tested on a memory task (Buschke selective reminding) and a test of visuo-spatial performance (figure copying). 3. No net change from baseline was seen in mean scores following arecoline infusion. However, the changes in performance on the two tasks were correlated (p < 0.02) over subjects at 10 min but not at 1.5 or 5.5 hr following the infusion. 4. This result suggests that although individual patients vary in their response to a given dose of arecoline, their responses are consistent across types of tasks. Thus the lack of a mean drug effect may be due to individual differences in response rather than to a lack of response. RP RAFFAELE, KC (reprint author), NIA,NEUROSCI LAB,BRAIN AGING & DEMENTIA SECT,BLDG 10,RM 6C414,BETHESDA,MD 20892, USA. NR 20 TC 19 Z9 19 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0278-5846 J9 PROG NEURO-PSYCHOPH JI Prog. Neuro-Psychopharmacol. Biol. Psychiatry PY 1991 VL 15 IS 5 BP 643 EP 648 DI 10.1016/0278-5846(91)90054-5 PG 6 WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GK887 UT WOS:A1991GK88700007 PM 1956992 ER PT J AU RUFF, MR SMITH, C KINGAN, T JAFFE, H HESELTINE, P GILL, MA MAYER, K PERT, CB BRIDGE, TP AF RUFF, MR SMITH, C KINGAN, T JAFFE, H HESELTINE, P GILL, MA MAYER, K PERT, CB BRIDGE, TP TI PHARMACOKINETICS OF PEPTIDE-T IN PATIENTS WITH ACQUIRED-IMMUNODEFICIENCY-SYNDROME (AIDS) SO PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY LA English DT Review DE AIDS; ANTIVIRAL; HUMAN; PEPTIDE-T; PHARMACOKINETICS; THERAPEUTICS ID BRAIN; VIRUS AB 1. The pharmacokinetics of Dalal-peptide T-NH2 (peptide T) was determined during phase I clinical trials in patients with acquired immunodeficiecy disease (AIDS) and AIDS related complex (ARC). Drug levels were determined by specific RIA, and in some cases with HPLC analysis, after intraveneous (i.v.) or intranasal (i.n.), via metered sprayer, administration. 2. The plasma kinetics appeared to be bi-phasic with a first compartment half-life of 30 to 60 minutes and a second plasma clearance rate of 4 to 6 hours, observed for both routes of administration. Peptide T, in one individual was confirmed to be present at 6 hrs in plasma, determined after HPLC isolation followed by specific RIA. 3. Bioavailability, determined for a 2 mg test dose in six individuals was 9.3 +/- 6.9 nmol/L. Peak plasma levels of 41 +/- 30 nmol/L after 10 mg i.n., 2.8 +/- 5.9 nmol/L after 2mg i.n., and 0.13 +/- 0.07 nmol/L after 0.4 mg i.n. were observed. In two individuals tested, peptide T was detected in CSF at levels 20% of the corresponding plasma level 90 and 145 minutes post i.v. administration. Peptide T was not detected in urine. I.N. administration was well tolerated for times up to 21 months. C1 NIMH,INTRAMURAL RES PROGRAM,BLDG 10,RM 4N-256,BETHESDA,MD 20892. INTEGRA INST,POTOMAC,MD. PEPTIDE DESIGN,GERMANTOWN,MD. FENWAY COMMUNITY HLTH CTR,BOSTON,MA 02115. UNIV SO CALIF,LOS ANGELES CTY MED CTR,DEPT INTERNAL MED,LOS ANGELES,CA 90033. UNIV SO CALIF,LOS ANGELES CTY MED CTR,DEPT PHARMACOL,LOS ANGELES,CA 90033. BROWN UNIV,PROVIDENCE,RI 02912. NR 10 TC 10 Z9 10 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0278-5846 J9 PROG NEURO-PSYCHOPH JI Prog. Neuro-Psychopharmacol. Biol. Psychiatry PY 1991 VL 15 IS 6 BP 791 EP 801 DI 10.1016/0278-5846(91)90008-O PG 11 WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GN297 UT WOS:A1991GN29700008 PM 1763193 ER PT J AU CLORE, GM GRONENBORN, AM AF CLORE, GM GRONENBORN, AM TI APPLICATIONS OF 3-DIMENSIONAL AND 4-DIMENSIONAL HETERONUCLEAR NMR-SPECTROSCOPY TO PROTEIN-STRUCTURE DETERMINATION SO PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY LA English DT Review ID NUCLEAR-MAGNETIC-RESONANCE; OVERHAUSER ENHANCEMENT SPECTROSCOPY; TWO-DIMENSIONAL NMR; SECONDARY STRUCTURE; PRACTICAL ASPECTS; C-13-LABELED PROTEINS; MOLECULAR TOPOLOGY; NOE SPECTROSCOPY; BINDING-PROTEIN; ROTATING-FRAME RP CLORE, GM (reprint author), NIDDKD,CHEM PHYS LAB,BLDG 2,BETHESDA,MD 20892, USA. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 78 TC 217 Z9 219 U1 2 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0079-6565 J9 PROG NUCL MAG RES SP JI Prog. Nucl. Magn. Reson. Spectrosc. PY 1991 VL 23 BP 43 EP 92 DI 10.1016/0079-6565(91)80002-J PN 1 PG 50 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical; Spectroscopy SC Chemistry; Physics; Spectroscopy GA FT353 UT WOS:A1991FT35300002 ER PT J AU TURNER, R KELLER, P AF TURNER, R KELLER, P TI ANGIOGRAPHY AND PERFUSION MEASUREMENTS BY NMR SO PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY LA English DT Review ID NUCLEAR-MAGNETIC-RESONANCE; CEREBRAL BLOOD-FLOW; VOLUME MR ANGIOGRAPHY; LIMITED-FLIP-ANGLE; INTRAVOXEL INCOHERENT MOTION; FREELY DIFFUSIBLE TRACER; PULSE-SEQUENCE; TRANSVERSE RELAXATION; HUMAN-BRAIN; INTRACRANIAL CIRCULATION C1 BARROW NEUROL INST,PHOENIX,AZ 85013. RP TURNER, R (reprint author), NIH,BEIP,INVIVO NMR RES CTR,BLDG 10,ROOM B1D125,BETHESDA,MD 20892, USA. RI Turner, Robert/C-1820-2008 NR 218 TC 12 Z9 12 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0079-6565 J9 PROG NUCL MAG RES SP JI Prog. Nucl. Magn. Reson. Spectrosc. PY 1991 VL 23 BP 93 EP 133 DI 10.1016/0079-6565(91)80003-K PN 1 PG 41 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical; Spectroscopy SC Chemistry; Physics; Spectroscopy GA FT353 UT WOS:A1991FT35300003 ER PT J AU SIMPSON, RT AF SIMPSON, RT TI NUCLEOSOME POSITIONING - OCCURRENCE, MECHANISMS, AND FUNCTIONAL CONSEQUENCES SO PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY LA English DT Review ID EUKARYOTIC DNA-REPLICATION; CEREVISIAE CHROMOSOMAL DNA; NUCLEASE-SENSITIVE REGIONS; ACID-PHOSPHATASE GENE; AFRICAN-GREEN MONKEY; ALPHA-SATELLITE DNA; RIBOSOMAL-RNA GENES; TRP1 RI CIRCLE; CHROMATIN STRUCTURE; SACCHAROMYCES-CEREVISIAE RP SIMPSON, RT (reprint author), NIDDKD,CELLULAR & DEV BIOL LAB,BETHESDA,MD 20892, USA. NR 128 TC 216 Z9 216 U1 0 U2 3 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0278-5846 J9 PROG NUCLEIC ACID RE JI Prog. Nucl. Acid Res. Mol. Biol. PY 1991 VL 40 BP 143 EP 184 DI 10.1016/S0079-6603(08)60841-7 PG 42 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GM715 UT WOS:A1991GM71500004 PM 2031082 ER PT B AU WAHL, SM ALLEN, JB BRANDES, ME AF WAHL, SM ALLEN, JB BRANDES, ME BE ACKERMAN, NR BONNEY, RJ WELTON, AF TI CYTOKINE MODULATION OF BACTERIAL-CELL WALL-INDUCED ARTHRITIS SO PROGRESS INFLAMMATION RESEARCH AND THERAPY SE AGENTS AND ACTIONS SUPPLEMENTS LA English DT Proceedings Paper CT 5TH INTERNATIONAL CONF OF THE INFLAMMATION RESEARCH ASSOC CY SEP 23-27, 1990 CL WHITE HAVEN, PA SP ABBOTT LABS, ALLERGAN PHARM, BIOMOL RES LABS, BOEHRINGER INGELHEIM PHARM, BRISTOL MYERS SQUIBB, BURROUGHS WELLCOME, CIBA GEIGY, CYTOGEN, DUPONT CO, GENENTECH RP WAHL, SM (reprint author), NIDR,CELLULAR IMMUNOL SECT,BETHESDA,MD 20892, USA. NR 0 TC 9 Z9 9 U1 0 U2 0 PU BIRKHAUSER VERLAG PI BASEL PA BASEL BN 3-7643-2529-1 J9 AGENT ACTION SUPPL PY 1991 VL 35 BP 29 EP 34 PG 6 WC Biochemistry & Molecular Biology; Pathology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Pathology; Pharmacology & Pharmacy GA BU85F UT WOS:A1991BU85F00005 PM 1781421 ER PT B AU ELING, TE GLASGOW, WC AF ELING, TE GLASGOW, WC BE SAMUELSSON, B RAMWELL, PW PAOLETTI, R FOLCO, G GRANSTROM, E TI TRANSDUCTION OF THE EPIDERMAL GROWTH-FACTOR MITOGENIC SIGNAL IN BALB/C 3T3 FIBROBLASTS INVOLVES LINOLEIC-ACID METABOLISM SO PROSTAGLANDINS AND RELATED COMPOUNDS, VOLS A AND B SE ADVANCES IN PROSTAGLANDIN THROMBOXANE AND LEUKOTRIENE RESEARCH LA English DT Review CT 7TH INTERNATIONAL CONF ON PROSTAGLANDINS AND RELATED COMPOUNDS CY MAY 28-JUN 01, 1990 CL FLORENCE, ITALY ID ARACHIDONIC-ACID; CELLS; STIMULATION; RELEASE RP ELING, TE (reprint author), NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-742-6 J9 ADV PROSTAG THROMB L PY 1991 VL 21 BP 843 EP 846 PG 4 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Research & Experimental Medicine; Science & Technology - Other Topics GA BS15B UT WOS:A1991BS15B00183 PM 1825423 ER PT J AU PYLKKANEN, L SANTTI, R NEWBOLD, R MCLACHLAN, JA AF PYLKKANEN, L SANTTI, R NEWBOLD, R MCLACHLAN, JA TI REGIONAL DIFFERENCES IN THE PROSTATE OF THE NEONATALLY ESTROGENIZED MOUSE SO PROSTATE LA English DT Article DE NEONATAL ESTROGENIZATION; ESTROGEN RECEPTORS; METAPLASIA; DYSPLASIA ID REPRODUCTIVE-TRACT; RAT; DIETHYLSTILBESTROL; ESTRADIOL; ANDROGEN; PROGESTERONE; EXPRESSION; HORMONES; LESIONS; BINDING AB Neonatal estrogenization of the mouse with diethylstilbestrol resulted in time-of-exposure and dose-dependent inhibition of the growth of the prostatic lobes observed at the age of 2 mon. The critical time was the days 1- 6 of postnatal life. In neonatally estrogenized (neoDES) mice, responses to 5-alpha-dihydrotestosterone in terms of nuclear H-3-thymidine labelling were altered concomitantly with the inhibition of growth and were in accordance with changes in the relative volumes of epithelium, glandular lumina, and interacinar stroma. Secondary estrogen treatment of neoDES mice with 17-beta-estradiol did not increase H-3-thymidine labelling in the prostate of control or neoDES mice. However, it induced squamous epithelial metaplasia in periurethral collecting ducts and proximal parts of coagulating glands of neoDES animals. In control mice only slight epithelial hyperplasia could be observed after similar treatment. Estrogen receptors, located immunocytochemically in nuclei of stromal cell, corresponded with the sites of increased estrogen sensitivity, observed as metaplastic transformation. When the neoDES animals aged, epithelial hyperplasia and dysplasia could be observed at distinct prostatic sites, ie, the periurethral collecting ducts and the coagulating glands and periurethral glands, and stromal inflammation become more extensive. Almost identical location of the epithelial changes and the altered estrogen response is suggestive of causal relationship. C1 NIEHS,REPROD & DEV TOXICOL LAB,RES TRIANGLE PK,NC 27709. RP PYLKKANEN, L (reprint author), UNIV TURKU,INST BIOMED,KIINAMYLLYNKATU 10,SF-20520 TURKU 52,FINLAND. NR 26 TC 70 Z9 70 U1 2 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0270-4137 J9 PROSTATE JI Prostate PY 1991 VL 18 IS 2 BP 117 EP 129 DI 10.1002/pros.2990180204 PG 13 WC Endocrinology & Metabolism; Urology & Nephrology SC Endocrinology & Metabolism; Urology & Nephrology GA FD240 UT WOS:A1991FD24000003 PM 2006118 ER PT B AU BAX, A IKURA, M KAY, LE BARBATO, G SPERA, S AF BAX, A IKURA, M KAY, LE BARBATO, G SPERA, S BE CHADWICK, DJ WIDDOWS, K TI MULTIDIMENSIONAL TRIPLE RESONANCE NMR-SPECTROSCOPY OF ISOTOPICALLY UNIFORMLY ENRICHED PROTEINS - A POWERFUL NEW STRATEGY FOR STRUCTURE DETERMINATION SO PROTEIN CONFORMATION SE CIBA FOUNDATION SYMPOSIA LA English DT Proceedings Paper CT SYMP ON PROTEIN CONFORMATION, IN HONOR OF SIR DAVID PHILLIPS ON HIS RETIREMENT FROM THE EXECUTIVE COUNCIL OF CIBA FOUNDATION CY JAN 22-24, 1991 CL CIBA FDN, LONDON, ENGLAND SP CIBA FDN HO CIBA FDN ID TWO-DIMENSIONAL NMR; STAPHYLOCOCCAL NUCLEASE; LARGER PROTEINS; 3-DIMENSIONAL STRUCTURE; SEQUENTIAL ASSIGNMENT; HETERONUCLEAR NMR; TRYPSIN-INHIBITOR; C-13; CALMODULIN; SPECTRA RP BAX, A (reprint author), NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892, USA. RI Barbato, Gaetano/G-4904-2011 NR 0 TC 24 Z9 24 U1 1 U2 1 PU JOHN WILEY & SONS LTD PI CHICHESTER PA CHICHESTER BN 0-471-92969-7 J9 CIBA F SYMP PY 1991 VL 161 BP 108 EP 135 PG 28 WC Biochemistry & Molecular Biology; Biophysics; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Biophysics; Chemistry GA BU50V UT WOS:A1991BU50V00008 PM 1814691 ER PT J AU SHOHAM, M PROCTOR, P HUGHES, D BALDWIN, ET AF SHOHAM, M PROCTOR, P HUGHES, D BALDWIN, ET TI CRYSTAL PARAMETERS AND MOLECULAR REPLACEMENT OF AN ANTICHOLERA TOXIN PEPTIDE COMPLEX SO PROTEINS-STRUCTURE FUNCTION AND GENETICS LA English DT Article DE ANTIBODY; FAB; ANTIGEN; CRYSTALLIZATION; X-RAY CRYSTALLOGRAPHY ID IMMUNOGLOBULIN FAB FRAGMENT; CHOLERA-TOXIN; SYNTHETIC PEPTIDES; B-SUBUNIT; MULTIDOMAIN PROTEINS; ANTIBODIES; CRYSTALLIZATION; ANTIGEN; NMR; NEUTRALIZATION AB TE33 is an Fab fragment of a monoclonal antibody raised against a 15-residue long peptide (CTP3), corresponding in sequence to residues 50-64 of the cholera toxin B subunit. Crystals of the complex between TE33 and CTP3 have been grown from 20% (w/v) polyethylene glycol-8000 at pH 4.0. The crystals are orthorhombic, space group P2(1)2(1)2, with unit cell dimensions a = 104.15, b = 110.61, and c = 40.68 angstrom. X-Ray data have been collected to a resolution of 2.3 angstrom. The asymmetric unit contains one molecule of Fab and one molecule of CTP3. The presence of CTP3 has been demonstrated by fluorescence quenching of the dissolved crystal after X-ray data collection. A molecular replacement solution was found based on the coordinates of DB3, an antiprogesterone Fab fragment. C1 NCI,FREDERICK CANC RES & DEV CTR,ABL,BASIC RES PROGRAM,CRYSTALLOG LAB,BETHESDA,MD 20892. RP SHOHAM, M (reprint author), CASE WESTERN RESERVE UNIV,SCH MED,DEPT BIOCHEM,2109 ADELBERT RD,CLEVELAND,OH 44106, USA. FU NCRR NIH HHS [S07 RR-05410-28] NR 24 TC 4 Z9 4 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0887-3585 J9 PROTEINS JI Proteins PY 1991 VL 11 IS 3 BP 218 EP 222 DI 10.1002/prot.340110306 PG 5 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA GM932 UT WOS:A1991GM93200005 PM 1749774 ER PT J AU ANDREWS, LC HARRISON, RW AF ANDREWS, LC HARRISON, RW TI MODELING CONFORMATIONAL CHANGE IN MACROMOLECULES AS AN ELASTIC-DEFORMATION SO PROTEINS-STRUCTURE FUNCTION AND GENETICS LA English DT Article DE CONFORMATION DIFFERENCE; STRAIN; ELASTICITY ID HEMOGLOBIN; RESOLUTION; PROTEIN AB Macromolecules are elastic bodies. Atomic structures are available for nucleic acids and proteins in two or more different conformations. It is a common practice to compare two structures by finding the best rigid body superposition of the molecules. This ignores possible deformations. There is useful information in the deviations from the rigid body superposition. If the deviations are considered to be elastic deformations of a common structure than it is possible to extract this information. Results are shown for comparisons of deoxyhemoglobin versus carbonmonoxyhemoglobin and for two different conformations of catabolite gene activator protein. C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,CRYSTALLOG LAB,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74101] NR 10 TC 3 Z9 3 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0887-3585 J9 PROTEINS JI Proteins PY 1991 VL 10 IS 2 BP 162 EP 170 DI 10.1002/prot.340100210 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FN762 UT WOS:A1991FN76200009 PM 1896429 ER PT J AU GUSTCHINA, A WEBER, IT AF GUSTCHINA, A WEBER, IT TI COMPARATIVE-ANALYSIS OF THE SEQUENCES AND STRUCTURES OF HIV-1 AND HIV-2 PROTEASES SO PROTEINS-STRUCTURE FUNCTION AND GENETICS LA English DT Article DE RETROVIRAL PROTEASES; ASPARTIC PROTEASES; HIV; PROTEASE INHIBITORS; SEQUENCE ANALYSIS ID VIRUS TYPE-1 PROTEASE; HUMAN-IMMUNODEFICIENCY; CRYSTAL-STRUCTURE; 3-DIMENSIONAL STRUCTURE; ASPARTIC PROTEINASES; RETROVIRAL PROTEASE; ESCHERICHIA-COLI; 2-A RESOLUTION; INHIBITOR; COMPLEX AB The different isolates available for HIV-1 and HIV-2 were compared for the region of the protease (PR) sequence, and the variations in amino acids were analyzed with respect to the crystal structure of HIV-1 PR with inhibitor. Based on the extensive homology (39 identical out of 99 residues), models were built of the HIV-2 PR complexed with two different aspartic protease inhibitors, acetylpepstatin and a renin inhibitor, H-261. Comparison of the HIV-1 PR crystal structure and the HIV-2 PR model structure and the analysis of the changes found in different isolates showed that correlated substitutions occur in the hydrophobic interior of the molecule and at surface residues involved in ionic or hydrogen bond interactions. The substrate binding residues of HIV-1 and HIV-2 PRs show conservative substitutions of four residues. The difference in affinity of HIV-1 and HIV-2 PRs for the two inhibitors appears to be due in part to the change of Val 32 in HIV-1 PR to Ile in HIV-2 PR. C1 NCI,FREDERICK CANC RES & DEV CTR,MACROMOLEC STRUCT LAB,ABL-BASIC RES PROGRAM,FREDERICK,MD 21702. FU PHS HHS [N01-C01-74101] NR 42 TC 81 Z9 82 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0887-3585 J9 PROTEINS JI Proteins PY 1991 VL 10 IS 4 BP 325 EP 339 DI 10.1002/prot.340100406 PG 15 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA FY794 UT WOS:A1991FY79400005 PM 1946342 ER PT J AU SCHULER, GD ALTSCHUL, SF LIPMAN, DJ AF SCHULER, GD ALTSCHUL, SF LIPMAN, DJ TI A WORKBENCH FOR MULTIPLE ALIGNMENT CONSTRUCTION AND ANALYSIS SO PROTEINS-STRUCTURE FUNCTION AND GENETICS LA English DT Article DE PATTERN RECOGNITION; SEQUENCE ALIGNMENT; ALGORITHMS; AMINO ACID SEQUENCES; MOLECULAR SEQUENCE DATA; PROTEINS; SOFTWARE ID SEQUENCE ALIGNMENT; PROTEIN SEQUENCES; PATTERN-RECOGNITION; ALGORITHM; TREES; DNA; PROGRAM AB Multiple sequence alignment can be a useful technique for studying molecular evolution, as well as for analyzing relationships between structure or function and primary sequence. We have developed for this purpose an interactive program, MACAW (Multiple Alignment Construction and Analysis Workbench), that allows the user to construct multiple alignments by locating, analyzing, editing, and combining "blocks" of aligned sequence segments. MACAW incorporates several novel features. (1) Regions of local similarity are located by a new search algorithm that avoids many of the limitations of previous techniques. (2) The statistical significance of blocks of similarity is evaluated using a recently developed mathematical theory. (3) Candidate blocks may be evaluated for potential inclusion in a multiple alignment using a variety of visualization tools. (4) A user interface permits each block to be edited by moving its boundaries or by eliminating particular segments, and blocks may be linked to form a composite multiple alignment. No completely automatic program is likely to deal effectively with all the complexities of the multiple alignment problem; by combining a powerful similarity search algorithm with flexible editing, analysis and display tools, MACAW allows the alignment strategy to be tailored to the problem at hand. RP SCHULER, GD (reprint author), NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20894, USA. NR 39 TC 963 Z9 966 U1 1 U2 8 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0887-3585 J9 PROTEINS JI Proteins PY 1991 VL 9 IS 3 BP 180 EP 190 DI 10.1002/prot.340090304 PG 11 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EW541 UT WOS:A1991EW54100003 PM 2006136 ER PT J AU KENDLER, KS OCHS, AL GORMAN, AM HEWITT, JK ROSS, DE MIRSKY, AF AF KENDLER, KS OCHS, AL GORMAN, AM HEWITT, JK ROSS, DE MIRSKY, AF TI THE STRUCTURE OF SCHIZOTYPY - A PILOT MULTITRAIT TWIN STUDY SO PSYCHIATRY RESEARCH LA English DT Article DE SCHIZOTYPY; ATTENTION; SMOOTH PURSUIT EYE MOVEMENTS; SCHIZOTYPAL PERSONALITY DISORDER; TWIN STUDIES ID PURSUIT EYE-MOVEMENTS; SCHIZOPHRENIC-PATIENTS; PERSONALITY-DISORDER; TRACKING DYSFUNCTIONS; PSYCHOSIS-PRONENESS; NORMAL POPULATION; SOCIAL ANHEDONIA; DSM-III; RELATIVES; FAMILY AB This report of a pilot study examines 29 pairs of twins from a population-based registry on whom four domains of schizotypy have been measured: personal interview using the Structured Interview for Schizotypy, self-report questionnaire formed from eight published self-report scales, attentional battery of eight individual tests, and root mean square error on smooth pursuit eye tracking. Analyzing the twins as individuals revealed two independent dimensions of clinically rated schizotypy (positive symptom schizotypy and negative symptom schizotypy) and two independent dimensions of self-rated schizotypy (positive trait schizotypy and trait anhedonia). Positive symptom schizotypy was highly correlated with positive trait schizotypy, but not with attentional dysfunction or eye-tracking error. By contrast, negative symptom schizotypy was significantly related to trait anhedonia, attentional dysfunction, and eye-tracking error. Correlations in monozygotic and dizygotic twins suggested that genetic factors were important in all four domains of schizotypy. Except for eye-tracking error, the results are more consistent with a dimensional than a "disease" model of schizotypy. Replication of these results with a larger group of subjects is needed. C1 NIMH,PSYCHOL & PSYCHOPATHOL LAB,BETHESDA,MD 20892. RP KENDLER, KS (reprint author), VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PSYCHIAT,BOX 710,MCV STN,RICHMOND,VA 23298, USA. OI Ochs, Alfred/0000-0003-3932-876X FU NIMH NIH HHS [MH-41953] NR 56 TC 153 Z9 153 U1 0 U2 8 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD JAN PY 1991 VL 36 IS 1 BP 19 EP 36 DI 10.1016/0165-1781(91)90114-5 PG 18 WC Psychiatry SC Psychiatry GA EX970 UT WOS:A1991EX97000002 PM 2017520 ER PT J AU ALBERT, PS ROSEN, LN ALEXANDER, JR ROSENTHAL, NE AF ALBERT, PS ROSEN, LN ALEXANDER, JR ROSENTHAL, NE TI EFFECT OF DAILY VARIATION IN WEATHER AND SLEEP ON SEASONAL AFFECTIVE-DISORDER SO PSYCHIATRY RESEARCH LA English DT Article DE AFFECTIVE DISORDER; SEASONAL AFFECTIVE DISORDER; HYPERSOMNIA; HARMONIC ANALYSIS; FEEDBACK ID PHOTOTHERAPY; TIME AB Analysis of daily self-ratings of energy for 10 patients diagnosed with seasonal affective disorder (SAD) revealed statistically significant seasonal patterns in eight patients (with all patients showing the most energy in the summer and the least energy in the winter). When weather was controlled for, the seasonal patterns in energy persisted in seven of the eight patients. In a lesser number of subjects (four), there were significant effects of weather after controlling for season; however, when the effects of weather on energy were examined separately for each season, 8 of the 10 subjects were found to be influenced by weather in at least one season. Daily sleep data showed statistically significant seasonal patterns in all 10 patients (with 6 subjects showing maximum sleep in winter and 4 in summer). As for the relationship between energy and sleep, a loss of energy appeared to predict longer sleep on that night and the next night (7 of 10 patients), whereas there was no evidence that prolonged sleep influenced energy on the following and subsequent days. C1 NIMH,CLIN PSYCHOBIOL BRANCH,OUTPATIENT STUDIES UNIT,BLDG 10,45-239,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NINCDS,BIOMETRY & FIELD STUDIES BRANCH,BETHESDA,MD 20892. UNIFORMED SERV UNIV HLTH SCI,BETHESDA,MD 20814. NR 16 TC 20 Z9 20 U1 1 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD JAN PY 1991 VL 36 IS 1 BP 51 EP 63 DI 10.1016/0165-1781(91)90117-8 PG 13 WC Psychiatry SC Psychiatry GA EX970 UT WOS:A1991EX97000005 PM 2017523 ER PT J AU ZAHN, TP NURNBERGER, JI BERRETTINI, WH ROBINSON, TN AF ZAHN, TP NURNBERGER, JI BERRETTINI, WH ROBINSON, TN TI CONCORDANCE BETWEEN ANXIETY AND AUTONOMIC NERVOUS-SYSTEM ACTIVITY IN SUBJECTS AT GENETIC RISK FOR AFFECTIVE-DISORDER SO PSYCHIATRY RESEARCH LA English DT Article DE AFFECTIVE DISORDER; ELECTRODERMAL ACTIVITY; HEART RATE; HIGH RISK; VULNERABILITY; STRESS ID ELECTRODERMAL ACTIVITY; DEPRESSION; UNIPOLAR; MARKER AB Correlations between electrodermal and heart rate indices of autonomic nervous system (ANS) activity and measures of state and trait anxiety were examined in 22 high-risk subjects, who had a parent with bipolar affective disorder, and in controls. Relatively consistent and significant correlations between anxiety and ANS "arousal" at rest and under stress were found in high-risk subjects, but not in controls. Relationships between electrodermal laterality and anxiety, and between electrodermal activity and heart rate, were also observed only in the high-risk group. The data suggest a unique concordance between various manifestations of anxiety in high-risk persons which may effectively increase the salience of stressful events and the sensitization to stress. C1 UNIV MARYLAND,DEPT AFAM STUDIES,CATONSVILLE,MD 21228. UNIV MARYLAND,DEPT PSYCHOL,CATONSVILLE,MD 21228. NIMH,PSYCHOL & PSYCHOPATHOL LAB,BETHESDA,MD 20892. INDIANA UNIV,MED CTR,INST PSYCHIAT RES,DEPT PSYCHIAT,INDIANAPOLIS,IN 46204. RP ZAHN, TP (reprint author), NIMH,CLIN NEUROGENET BRANCH,BLDG 10,RM 4C110,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. OI Nurnberger, John/0000-0002-7674-1767 NR 40 TC 21 Z9 21 U1 2 U2 7 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD JAN PY 1991 VL 36 IS 1 BP 99 EP 110 DI 10.1016/0165-1781(91)90121-5 PG 12 WC Psychiatry SC Psychiatry GA EX970 UT WOS:A1991EX97000009 PM 2017527 ER PT J AU BACHEVALIER, J HAGGER, C AF BACHEVALIER, J HAGGER, C TI SEX-DIFFERENCES IN THE DEVELOPMENT OF LEARNING-ABILITIES IN PRIMATES SO PSYCHONEUROENDOCRINOLOGY LA English DT Review ID DEVELOPING RHESUS-MONKEY; INTERTRIAL INTERVALS; GONADAL-HORMONES; BRAIN; ESTROGEN; LESIONS; DIFFERENTIATION; TESTOSTERONE; HABITUATION; PITUITARY AB The role of steroid gonadal hormones in promoting sex differences in reproductive behaviors has been thoroughly studied in numerous mammalian species. More recent experiments have indicated that the presence or absence of steroid hormones during the critical period of brain differentiation likewise might promote the development of sex differences in cognitive abilities. Studies in infant rhesus monkeys have demonstrated that there exist sex differences in learning abilities that can be altered by perinatal hormonal manipulations, suggesting that testosterone might be a crucial factor responsible for the development of sex differences in cognitive styles. In addition, neonatal lesion studies have shown that the cortical areas mediating specific learning abilities mature at different rates in male and female infant monkeys. These findings support the view that the perinatal hormonal environment can affect the rate of brain maturation by influencing neuronal connectivity at the cortical level. The combined data from sex differences in learning abilities in human infants and their reversibility in endocrinological syndromes suggest that testosterone may affect the maturation of the human brain in a manner similar to that demonstrated in nonhuman primates. C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. NR 65 TC 65 Z9 67 U1 2 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4530 J9 PSYCHONEUROENDOCRINO JI Psychoneuroendocrinology PY 1991 VL 16 IS 1-3 BP 177 EP 188 DI 10.1016/0306-4530(91)90077-7 PG 12 WC Endocrinology & Metabolism; Neurosciences; Psychiatry SC Endocrinology & Metabolism; Neurosciences & Neurology; Psychiatry GA GF718 UT WOS:A1991GF71800012 PM 1961838 ER PT J AU BITAR, MS OTA, M LINNOILA, M SHAPIRO, BH AF BITAR, MS OTA, M LINNOILA, M SHAPIRO, BH TI MODIFICATION OF GONADECTOMY-INDUCED INCREASES IN BRAIN MONOAMINE METABOLISM BY STEROID-HORMONES IN MALE AND FEMALE RATS SO PSYCHONEUROENDOCRINOLOGY LA English DT Article ID DOPAMINERGIC-NEURONS; LUTEINIZING-HORMONE; ESTROGEN-TREATMENT; CATECHOLAMINE TURNOVER; RECEPTOR SENSITIVITY; TYROSINE-HYDROXYLASE; OVARIAN STEROIDS; SEX-DIFFERENCES; ESTRADIOL; TESTOSTERONE AB Concentrations of monoamines (dopamine, DA; serotonin, 5-HT) and their major metabolites (homovanillic acid - HVA; dihydroxyphenylacetic acid - DOPAC; 5-hydroxyindolacetic acid - 5-HIAA) were measured in selected brain areas of chronically gonadectomized, steroid- or oil-treated male and female rats. Concentrations of DOPAC and HVA were markedly increased in the hypothalamus (male, female), striatum (male, female) and brainstem (male) following gonadectomy, whereas the levels of DA remained unaltered in most of the brain areas examined. Most of the changes were reversed or attenuated by chronic estradiol (EB) substitution. In contrast, chronic treatment with physiological concentrations of testosterone (TP) reduced indexes of DA turnover only in the striatum of ovariectomized (OVX) and brainstem of orchidectomized (ORDX) rats. ORDX-related increases in striatal levels of DOPAC and HVA were not reversed by either EB or TP. ORDX increased the levels of 5-HIAA (hypothalamus, striatum) and decreased those of 5-HT (hypothalamus, hippocampus). These changes were reversed by chronic treatment with either TP or EB. Brain metabolism of 5-HT remained unaltered following OVX. Gonadectomy and chronic steroid replacement therapy appear to alter brain monoamine metabolism in a brain region and sex-dependent manner. Our data demonstrate that gonadectomy-related increases in the activity of brain monoaminergic neurons in both male and female rats was attenuated more effectively with physiological concentrations of estradiol than with testosterone. Insensitivity of monoaminergic neurons in a number of brain areas (e.g., hypothalamus, striatum) to the action of testosterone was evident in both sexes. C1 NIAAA,DICBR,NATL LAB CLIN STUDIES,BETHESDA,MD. UNIV PENN,SCH VET MED,DEPT ANIM BIOL,BIOCHEM LABS,PHILADELPHIA,PA 19104. RP BITAR, MS (reprint author), PENN COLL PODIATR MED,DEPT PHYSIOL SCI,8TH & RACE ST,PHILADELPHIA,PA 19107, USA. FU NICHD NIH HHS [HD-16358] NR 57 TC 48 Z9 48 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4530 J9 PSYCHONEUROENDOCRINO JI Psychoneuroendocrinology PY 1991 VL 16 IS 6 BP 547 EP 557 DI 10.1016/0306-4530(91)90038-U PG 11 WC Endocrinology & Metabolism; Neurosciences; Psychiatry SC Endocrinology & Metabolism; Neurosciences & Neurology; Psychiatry GA HJ389 UT WOS:A1991HJ38900009 PM 1725825 ER PT J AU MUNTANER, C KUMOR, KM NAGOSHI, C JAFFE, JH AF MUNTANER, C KUMOR, KM NAGOSHI, C JAFFE, JH TI EFFECTS OF NIFEDIPINE PRETREATMENT ON SUBJECTIVE AND CARDIOVASCULAR-RESPONSES TO INTRAVENOUS COCAINE IN HUMANS SO PSYCHOPHARMACOLOGY LA English DT Article DE NIFEDIPINE; COCAINE; BLOOD PRESSURE; HEART RATE ID CALCIUM; ANTIDOTE; ABUSE AB The effects of oral nifedipine pretreatment on subjective and cardiovascular responses to intravenous cocaine infusions were studied in cocaine-using volunteers. Nifedipine, 10 mg or placebo, was administered 20-25 min before placebo, 20 mg, or 40 mg cocaine, using a repeated measures randomized double-blind design. The variables measured were self-reported subjective effects, general behavior rated by two observers, blood pressure and heart rate. Cocaine produced the expected dose-related effects on subjective and cardiovascular measures. Nifedipine pretreatment attenuated some subjective effects of cocaine. Nifedipine directly reduced blood pressure but did not antagonize the effects of cocaine on blood pressure. These findings suggest that dihydropyridine calcium channel modulators may be useful compounds in the clinical management of cocaine users. C1 NIDA,ADDICT RES CTR,BALTIMORE,MD 21224. RI Muntaner, C/A-5043-2010 NR 25 TC 32 Z9 32 U1 0 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 105 IS 1 BP 37 EP 41 DI 10.1007/BF02316861 PG 5 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GB768 UT WOS:A1991GB76800006 PM 1745709 ER PT J AU EVONIUK, GE HERTZMAN, RP SKOLNICK, P AF EVONIUK, GE HERTZMAN, RP SKOLNICK, P TI A RAPID METHOD FOR EVALUATING THE BEHAVIORAL-EFFECTS OF PHENCYCLIDINE-LIKE DISSOCIATIVE ANESTHETICS IN MICE SO PSYCHOPHARMACOLOGY LA English DT Article DE PHENCYCLIDINE; DIZOLCIPINE; GLYCINE; DISSOCIATIVE ANESTHETICS; SIGMA RECEPTORS ID DISCRIMINATIVE STIMULUS PROPERTIES; D-ASPARTATE ANTAGONIST; NERVOUS-SYSTEM; RECEPTORS; MK-801; ACID; RATS; GLYCINE; BINDING; PIGEONS AB A simple and rapid method for detecting the behavioral effects of phencyclidine and related dissociative anesthetics is described. Dissociative anesthetics such as phencyclidine (PCP) and dizolcipine, which bind with high affinities at N-methyl-D-aspartate (NMDA) coupled cation channels ("PCP receptors"), produced a dose-related increase in the percentage of mice that fell from a 1.5 cm deep circular arena mounted on a 60 cm platform. A similar behavior was not manifest by other classes of compounds examined including competitive NMDA antagonists, an antagonist at strychnine-insensitive glycine receptors, and sigma-receptor ligands with moderate to low affinities for PCP receptors. Pretreatment of mice with glycine reduced in a dose-dependent manner the percentage of falls elicited by a maximally effective dose of dizolcipine. This simple procedure may prove useful for both the rapid detection of dissociative anesthetics and evaluation of putative PCP antagonists. RP EVONIUK, GE (reprint author), NIDDK,LN,NEUROSCI LAB,BLDG 8,ROOM 111,BETHESDA,MD 20892, USA. NR 31 TC 22 Z9 22 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 105 IS 1 BP 125 EP 128 DI 10.1007/BF02316874 PG 4 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GB768 UT WOS:A1991GB76800019 PM 1660607 ER PT J AU KATZ, JL SHARPE, LG JAFFE, JH SHORES, EI WITKIN, JM AF KATZ, JL SHARPE, LG JAFFE, JH SHORES, EI WITKIN, JM TI DISCRIMINATIVE STIMULUS EFFECTS OF INHALED COCAINE IN SQUIRREL-MONKEYS SO PSYCHOPHARMACOLOGY LA English DT Article DE COCAINE; INHALATION; ROUTE OF ADMINISTRATION; DISCRIMINATIVE-STIMULUS EFFECTS; BEHAVIOR; SQUIRREL MONKEYS ID PYROLYSIS; SMOKING AB Squirrel monkeys (N = 4) were trained with food reinforcement to press one of two levers after administration of IV cocaine (0.3 or 1.0 mg/kg) or the other lever after saline. After training, IV cocaine (0.03-3.0 mg/kg) produced dose-related increases in the percentage of responses on the cocaine lever (ED50 = 0.15 mg/kg). Cocaine delivered IM also produced dose-related increases in cocaine-appropriate responding (ED50 = 0.32 mg/kg), but was approximately half as potent as IV cocaine. Similar relative potency relations were obtained for decreases in response rates produced by cocaine. Prior to some sessions subjects were placed in a Plexiglas(R) chamber and exposed for 60 s to cocaine vapor created with an ultrasonic nebulizer. Exposure to vapor from cocaine solutions (1.0-30.0 mg/ml) produced concentration-dependent increases in cocaine-appropriate responding and decreases in response rates. Exposure to vapor from a 30 mg/ml concentration produced virtually exclusive cocaine-appropriate responding. Concentration-effect curves for inhaled cocaine were similar to dose-effect curves obtained when cocaine was administered by the other routes. The time course of the minimally effective concentration of inhaled cocaine was compared to that of the minimally effective doses of systemically administered cocaine. Inhaled cocaine had a duration of action longer than IV cocaine. The results indicate that inhaled cocaine vapor has effects qualitatively similar to those of IV cocaine, and may have a duration of action longer than that of an IV cocaine dose producing a similar degree of drug-appropriate responding. RP KATZ, JL (reprint author), NIDA,ADDICT RES CTR,PRECLIN PHARMACOL BRANCH,PSYCHOBIOL LAB,POB 5180,BALTIMORE,MD 21224, USA. OI Katz, Jonathan/0000-0002-1068-1159 NR 16 TC 7 Z9 7 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 105 IS 3 BP 317 EP 321 DI 10.1007/BF02244424 PG 5 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GJ525 UT WOS:A1991GJ52500003 PM 1798829 ER PT J AU WINSLOW, JT INSEL, TR AF WINSLOW, JT INSEL, TR TI SEROTONERGIC MODULATION OF THE RAT PUP ULTRASONIC ISOLATION CALL - STUDIES WITH 5HT1 AND 5HT2 SUBTYPE-SELECTIVE AGONISTS AND ANTAGONISTS SO PSYCHOPHARMACOLOGY LA English DT Article DE ATTACHMENT BEHAVIOR; VOCALIZATION; INFANT RATS; SEROTONIN; DEVELOPMENT; ANXIETY ID ISOLATION DISTRESS; RECEPTOR COMPLEX; BINDING-SITES; M-CPP; RESPONSES; BRAIN; VOCALIZATIONS; MEDIATION; RADIOLIGAND; 8-OH-DPAT AB A modulatory role for serotonin has been described for the development and expression of the ultrasonic call of infant rat pups during brief maternal separations. In previous studies, serotonin reuptake inhibitors selectively reduced the rate of calling following acute administration to 9-11-day-old pups and a serotonin neurotoxin (MDMA) systematically disrupted the development of ultrasonic vocalizations but not other measures of motor development. In the current studies, we extended our investigations to include drugs with purported receptor subtype selectivities. Consistent with previous reports, acute administration of 5HT1A agonists buspirone and 8-OH-DPAT ((+/-)-8-hydroxy-2-(di-N-propylamino)tetralin) reduced the rate of calling at doses which did not affect motor activity or core body temperature. The rate reducing effects of buspirone persisted up to 1 but not 2 h after injection. Administration of purported 5HT1B receptor agonists, CGS12066B (7-trifluoromethyl-4(4-methyl-1-piperazinyl)-pyrrolo[1,2-a] quinoxaline) and TFMPP (1-[3-fluoromethyl)phenyl]-piperazine) increased the rate of calling depending on the specificity of the drug for the 5HT1B receptor. d,l-Propranolol, a 5HT1 receptor antagonist, blocked the effects of both 8-OH-DPAT and TFMPP . m-CPP (1-(3-chlorophenyl)piperazine) and DOI ((+/-)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane), drugs with putative actions at 5HT1C and 5HT2 receptor sites both decreased calling but differed according to their effects on motor activity. Ritanserin, a 5HT2 and 5HT1C antagonist, produced a dose-related increase in call rate. A dose of ritanserin with no apparent intrinsic effects effectively antagonized DOI rate reducing effects but potentiated the rate reducing effects of m-CPP. These data extend previous studies demonstrating a role for serotonin in the expression of rat pup separation calls and further demonstrate that 5HT may increase or decrease calling depending on with receptor subtype is affected. RP WINSLOW, JT (reprint author), NIMH,ANIM CTR,CLIN SCI LAB,POB 289,POOLESVILLE,MD 20837, USA. NR 48 TC 62 Z9 62 U1 0 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 105 IS 4 BP 513 EP 520 DI 10.1007/BF02244372 PG 8 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GN105 UT WOS:A1991GN10500011 PM 1771219 ER PT J AU WITKIN, JM SCHINDLER, CW TELLA, SR GOLDBERG, SR AF WITKIN, JM SCHINDLER, CW TELLA, SR GOLDBERG, SR TI INTERACTION OF HALOPERIDOL AND SCH-23390 WITH COCAINE AND DOPAMINE RECEPTOR SUBTYPE-SELECTIVE AGONISTS ON SCHEDULE-CONTROLLED BEHAVIOR OF SQUIRREL-MONKEYS SO PSYCHOPHARMACOLOGY LA English DT Article DE COCAINE; DOPAMINE RECEPTOR SUBTYPES; BEHAVIORAL EFFECTS; SQUIRREL MONKEYS ID DISCRIMINATIVE STIMULUS PROPERTIES; ANTAGONIST SCH-23390; NONHUMAN-PRIMATES; D-1 ANTAGONIST; DRUGS; BENZAZEPINE; BLOCKADE AB Involvement of D1 and D2 dopamine receptors in the effects of cocaine on schedule-controlled behavior was evaluated in squirrel monkeys responding under a multiple fixed-interval 5-min, fixed-ratio 10 schedule (mult FI FR) of food delivery. Cocaine and the D2 agonist quinpirole increased responding under the FI at certain doses and disrupted the temporal patterning of behavior. Higher doses of these drugs decreased responding. In contrast, the D1 agonist SKF 38393 was devoid of behavioral activity up to 10 mg/kg where response suppression was obtained without significant modification of the temporal distribution of responding. The D2 antagonist haloperidol (0.001-0.03 mg/kg) did not alter the behavioral effects of cocaine up to doses that had pronounced behavioral effects on their own. However, haloperidol attenuated the behavioral effects of quinpirole. In contrast, the D1 antagonist SCH 23390 partially attenuated the response rate-suppressant effects of cocaine without blocking cocaine-induced disruptions of temporal response patterning. SCH 23390 did not antagonize the behavioral effects of SKF 38393. These results suggest that independent stimulation of either D1 or D2 receptors alone does not play a major role in the effects of cocaine on schedule-controlled behavior of squirrel monkeys. RP WITKIN, JM (reprint author), NIDA,ADDICT RES CTR,PRECLIN PHARMACOL BRANCH,POB 5180,BALTIMORE,MD 21224, USA. NR 27 TC 21 Z9 21 U1 3 U2 3 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 104 IS 4 BP 425 EP 431 DI 10.1007/BF02245644 PG 7 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GA171 UT WOS:A1991GA17100002 PM 1838199 ER PT J AU GRANT, KA BARRETT, JE AF GRANT, KA BARRETT, JE TI BLOCKADE OF THE DISCRIMINATIVE STIMULUS EFFECTS OF ETHANOL WITH 5-HT3-RECEPTOR ANTAGONISTS SO PSYCHOPHARMACOLOGY LA English DT Article DE ETHANOL; SEROTONIN RECEPTORS; DRUG-DISCRIMINATION; SEROTONIN ANTAGONISTS; ETHANOL ANTAGONIST; 5-HT3 ID 5-HT3 RECEPTOR ANTAGONIST; DOPAMINE RELEASE; BRAIN; STIMULATION AB The ability of selective 5-HT3 receptor antagonists to block the discriminative stimulus effects of ethanol was investigated in pigeons trained with food reinforcement to discriminate ethanol (1.5 g/kg; IG) from water. The 5-HT3 receptor antagonists that are substituted tropines, ICS 205-930 (0.1-0.56 mg/kg) and MDL 72222 (3.0-17.0 mg/kg), blocked ethanol-appropriate responding, in a dose-dependent manner, suggesting that some of the discriminative stimulus effects of ethanol are mediated via the 5-HT3 receptor. The blockade the discriminative stimulus effects of ethanol occurred in the presence of approximately 25-40 mM blood ethanol levels. Furthermore, the ethanol dose-effect function was shifted to the right by increasing doses of MDL 72222, suggesting a surmountable antagonism of the discriminative stimulus effects of ethanol. However, the benzamide zacopride (0.56-1.7 mg/kg), which is also a 5-HT3 receptor antagonist, did not block the discriminative stimulus effects of ethanol. In addition, the dopaminergic antagonist haloperidol and the 5-HT2 receptor antagonist ketanserin also failed to block the ethanol discrimination. The results suggest that 5-HT3 mediated neurotransmission is an important component of ethanol's discriminative stimulus effects, but that the structural characteristics of the selective 5-HT3 receptor antagonists influence their ability to block this action of ethanol. Furthermore, these findings implicate a significant role of 5-HT3 activity in the behavioral effects of ethanol that may provide a pharmacological means for therapeutic intervention of alcohol abuse. C1 UNIFORMED SERV UNIV HLTH SCI,DEPT PSYCHIAT,BETHESDA,MD 20814. RP GRANT, KA (reprint author), NIAAA,SPECIAL PROJECTS UNIT,12501 WASHINGTON AVE,ROCKVILLE,MD 20852, USA. NR 30 TC 107 Z9 107 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 104 IS 4 BP 451 EP 456 DI 10.1007/BF02245648 PG 6 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GA171 UT WOS:A1991GA17100006 PM 1780414 ER PT J AU GEORGE, FR PORRINO, LJ RITZ, MC GOLDBERG, SR AF GEORGE, FR PORRINO, LJ RITZ, MC GOLDBERG, SR TI INBRED RAT STRAIN COMPARISONS INDICATE DIFFERENT SITES OF ACTION FOR COCAINE AND AMPHETAMINE LOCOMOTOR STIMULANT EFFECTS SO PSYCHOPHARMACOLOGY LA English DT Article DE LOCOMOTOR ACTIVITY; COCAINE; AMPHETAMINE; BEHAVIOR GENETICS; RATS; DOPAMINERGIC-D1; DOPAMINERGIC-D2; DOPAMINE UPTAKE ID DOPAMINE-RECEPTORS; BRAIN; MICE; BINDING; SENSITIVITY; STRIATUM; SYSTEMS; SCOPOLAMINE; ALCOHOL; LIGAND AB Cocaine and amphetamine produce several behavioral effects, most notably locomotor stimulation. Biochemically, evidence suggests specific involvement of dopaminergic systems, although not necessarily identical sites, in mediating cocaine- and amphetamine-induced locomotor stimulation. This study examined the effects of cocaine or amphetamine on locomotor activity in rats from the ACI, F344, LEW and NBR inbred strains. Dose-dependent increases in locomotor activity were found for both drugs in all strains. However, large potency and efficacy differences were found. Further, significant strain by drug interactions were found, in that the strain rank order for stimulant response to the two drugs was not identical. Since striatal dopaminergic neurons influence locomotor activity, we also assessed ligand affinity and receptor density of dopamine transporters and dopaminergic D1 and D2 receptors in striatal tissue from these same strains of rats. No differences in these receptor binding parameters were found. These findings support the conclusion that these two drugs produce their locomotor stimulant effects through different sites of action, and that genetic differences in response to these drugs at the behavioral level do not appear to be mediated significantly by differences in structure or number of striatal dopaminergic sites. The further use of genetic methods, however, may aid in determining the specific sites of action of these widely used stimulant drugs. C1 NIDA,ADDICT RES CTR,PRECLIN PHARMACOL BRANCH,BALTIMORE,MD 21224. NINCDS,BRAIN IMAGING UNIT,BETHESDA,MD 20892. RP GEORGE, FR (reprint author), UNIV NEW MEXICO,CTR ALCOHOLISM SUBST ABUSE & ADDICT,DEPT PSYCHOL,ALBUQUERQUE,NM 87131, USA. FU NIAAA NIH HHS [AA-07754] NR 47 TC 72 Z9 72 U1 5 U2 6 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 104 IS 4 BP 457 EP 462 DI 10.1007/BF02245649 PG 6 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA GA171 UT WOS:A1991GA17100007 PM 1838200 ER PT J AU KATZ, JL WITKIN, JM AF KATZ, JL WITKIN, JM TI BEHAVIORAL-EFFECTS OF COCAINE ALONE AND IN COMBINATION WITH SELECTIVE DOPAMINE ANTAGONISTS IN THE SQUIRREL-MONKEY SO PSYCHOPHARMACOLOGY LA English DT Article ID DISCRIMINATIVE STIMULUS PROPERTIES; SCHEDULE-CONTROLLED BEHAVIOR; RHESUS-MONKEYS; HALOPERIDOL; SCH-23390; DRUGS; D1; BLOCKADE; ANALOGS; RATS RP KATZ, JL (reprint author), NIDA,ADDICT RES CTR,PSYCHOBIOL LAB,POB 5180,BALTIMORE,MD 21224, USA. OI Katz, Jonathan/0000-0002-1068-1159 NR 36 TC 13 Z9 13 U1 1 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 103 IS 1 BP 33 EP 40 DI 10.1007/BF02244070 PG 8 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA EL783 UT WOS:A1991EL78300005 PM 2006242 ER PT J AU HEISHMAN, SJ HENNINGFIELD, JE AF HEISHMAN, SJ HENNINGFIELD, JE TI DISCRIMINATIVE STIMULUS EFFECTS OF D-AMPHETAMINE, METHYLPHENIDATE, AND DIAZEPAM IN HUMANS SO PSYCHOPHARMACOLOGY LA English DT Article DE HUMAN DRUG DISCRIMINATION; DRUG ABUSE; D-AMPHETAMINE; DIAZEPAM; METHYLPHENIDATE ID DRUG DISCRIMINATION; COLOR-TRACKING; QUANTAL NATURE; PHENMETRAZINE; BEHAVIOR; PIGEONS; RATS AB Eight male community volunteers, who reported current psychomotor stimulant use, were trained to discriminate between the presence and absence of orally administered d-amphetamine 30 mg. During daily experimental sessions, in which a single drug dose or placebo was tested, physiological and subjective measures were assessed and subjects indicated their discrimination by responding on an operant color-tracking procedure. During four test of acquisition sessions, discriminative responding indicated that all subjects learned the discrimination, and d-amphetamine produced physiological and subjective effects typical of psychomotor stimulants. Generalization testing then followed in which dose-response curves were determined for the following drugs: d-amphetamine (3.75, 7.5, 15 and 30 mg), diazepam (5, 10, 20 and 40 mg), and methylphenidate (7.5, 15, 30 and 60 mg). d-Amphetamine and methylphenidate produced dose-related increases in d-amphetamine-appropriate responding, whereas no dose of diazepam substituted for d-amphetamine in any subject. d-Amphetamine and methylphenidate produced a similar pattern of subjective changes, including increased ratings of euphoria and drug liking and decreased sedation. In contrast, diazepam increased subjective scales of sedation and dysphoria. These results are consistent with similar studies testing animals and humans and demonstrate the utility of human drug discrimination research as an integral component of drug abuse liability testing. RP HEISHMAN, SJ (reprint author), NIDA,ADDICT RES CTR,CLIN PHARMACOL BRANCH,POB 5180,BALTIMORE,MD 21224, USA. NR 29 TC 78 Z9 78 U1 1 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 103 IS 4 BP 436 EP 442 DI 10.1007/BF02244241 PG 7 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA FD582 UT WOS:A1991FD58200001 PM 2062984 ER PT J AU HIGLEY, JD SUOMI, SJ LINNOILA, M AF HIGLEY, JD SUOMI, SJ LINNOILA, M TI CSF MONOAMINE METABOLITE CONCENTRATIONS VARY ACCORDING TO AGE, REARING, AND SEX, AND ARE INFLUENCED BY THE STRESSOR OF SOCIAL SEPARATION IN RHESUS-MONKEYS SO PSYCHOPHARMACOLOGY LA English DT Article DE RHESUS MONKEYS; SEX; PEER-REARING; ANXIETY; 5-HYDROXYINDOLEACETIC ACID (5-HIAA); HOMOVANILLIC ACID (HVA); 3-METHOXY-4-HYDROXYPHENYLGLYCOL (MHPG) ID HOMOVANILLIC-ACID CONCENTRATIONS; CEREBROSPINAL-FLUID; 5-HYDROXYINDOLEACETIC ACID; DOPAMINE; PRIMATES; AGGRESSION; SEROTONIN; CHILDREN; BRAIN AB In humans, CSF monoamine metabolite concentrations have been shown to vary as a complex function of age, sex, psychiatric diagnosis, and stress. To test for such relationships in rhesus monkeys, 28 subjects, reared either in anxiety producing peer-only groups or in mother-infant dyads, were studied at 6, 18 or 50 months of age. Each monkey underwent a series of four 4-day social separations, each followed by 3 days of reunion. Prior to and during the first and fourth separations, CSF was obtained from the cisterna magna and assayed for the serotonin metabolite 5-HIAA, the dopamine metabolite HVA, and the norepinephrine metabolite MHPG. CSF 5-HIAA showed an age-related decline which was greater in the mother-reared subjects. Peer-only-reared males had an increased 5-HIAA concentration relative to females, and higher 5-HIAA levels than mother-reared males. MHPG was also higher in peer-only-reared monkeys than in mother-reared subjects at all ages. In both groups HVA declined across the three ages, and MHPG increased from the 18- to the 50-month measurements. Both MHPG and 5-HIAA concentrations increased during the initial social separation, although only MHPG remained elevated across the repeated separations; HVA, on the other hand declined during social separation. These results are discussed in terms of established anxiety and aggression differences between peer-only and mother-reared monkeys. C1 NICHHD,COMPARAT ETHOL LAB,BETHESDA,MD 20892. NIAAA,DICBR,CLIN STUDIES LAB,BETHESDA,MD 20892. NR 45 TC 150 Z9 150 U1 1 U2 8 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 103 IS 4 BP 551 EP 556 DI 10.1007/BF02244258 PG 6 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA FD582 UT WOS:A1991FD58200018 PM 1712115 ER PT J AU ROSENTHAL, NE ALBERT, PS OWEN, DA AF ROSENTHAL, NE ALBERT, PS OWEN, DA TI SEASONAL AFFECTIVE-DISORDER AND ITS TREATMENT SO PSYCHOPHARMACOLOGY LA English DT Meeting Abstract C1 NIMH,CLIN PSYCHOBIOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PY 1991 VL 103 IS 4 BP B6 EP B6 PG 1 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA FD582 UT WOS:A1991FD58200036 ER PT J AU WILKINS, JN SHANER, AL PATTERSON, CM SETODA, D GORELICK, D AF WILKINS, JN SHANER, AL PATTERSON, CM SETODA, D GORELICK, D TI DISCREPANCIES BETWEEN PATIENT REPORT, CLINICAL-ASSESSMENT, AND URINE ANALYSIS IN PSYCHIATRIC-PATIENTS DURING INPATIENT ADMISSION SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 30TH ANNUAL MEETING OF THE NEW CLINICAL DRUG EVALUATION UNIT CY MAY 29-JUN 01, 1990 CL KEY BISCAYNE, FL SP NIMH, DIV CLIN RES ID DRUG-USE; SELF-REPORTS; ALCOHOLICS; ABUSE; DISORDERS; WOMEN AB Self-report and clinical assessment of substance use were compared with urine analysis results in 56 male patients consecutively admitted for inpatient psychiatric treatment. All subjects received DSM-III-R Axis I diagnosis and were classified into diagnostic groups. Urine samples were tested for cocaine, marijuana, opiates, phencyclidine (PCP), amphetamines, and barbiturates. Thirty-five of the 56 patients (62%) produced urine samples that were positive for at least 1 substance of abuse. Of this group, 15 patients (27% of total sample) denied substance use during the week prior to admission. In addition, the admitting physician did not identify intoxication in 23 of the 35 patients (66%) with postive urines. The admitting physician's assessment matched the patient's answers regarding recent substance use in 79 percent of the patients. This association was especially apparent with the 26 patients who denied recent substance use, all but one of whom received a drug-negative assessment from the admitting physician. C1 VET ADM MED CTR,PALO ALTO,CA 94304. UNIV MARYLAND,SCH MED,NIDA ADDICT RES CTR,BALTIMORE,MD 21201. RP WILKINS, JN (reprint author), VET ADM MED CTR,BRENTWOOD DIV,ROOM 2A,BLDG 256,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 15 TC 24 Z9 24 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1991 VL 27 IS 2 BP 149 EP 154 PG 6 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA GA037 UT WOS:A1991GA03700012 PM 1924662 ER PT J AU MANJI, HK LENOX, RH POTTER, WZ AF MANJI, HK LENOX, RH POTTER, WZ TI RECEPTOR-G PROTEIN 2ND-MESSENGER INTERACTION - A POTENTIAL TARGET SITE FOR LITHIUM SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material C1 UNIV VERMONT,COLL MED,BURLINGTON,VT 05405. NIMH,ROCKVILLE,MD 20857. RP MANJI, HK (reprint author), NIMH,EXPTL THERAPEUT BRANCH,CLIN PHARMACOL SECT,BLDG 10,ROOM 2046,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1991 VL 27 IS 3 BP 183 EP 184 PG 2 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA GP897 UT WOS:A1991GP89700002 ER PT J AU MANJI, HK BITRAN, JA MASANA, MI CHEN, G HSIAO, JK RISBY, ED RUDORFER, MV POTTER, WZ AF MANJI, HK BITRAN, JA MASANA, MI CHEN, G HSIAO, JK RISBY, ED RUDORFER, MV POTTER, WZ TI SIGNAL TRANSDUCTION MODULATION BY LITHIUM - CELL-CULTURE, CEREBRAL MICRODIALYSIS AND HUMAN STUDIES SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 29TH ANNUAL MEETING OF THE AMERICAN COLLEGE OF NEUROPSYCHOPHARMACOLOGY CY DEC, 1990 CL SAN JUAN, PR SP AMER COLL NEUROPSYCHOPHARM ID ADENYLATE-CYCLASE SYSTEM; CYCLIC-AMP ACCUMULATION; PERTUSSIS TOXIN; RAT-BRAIN; PROTEIN; INHIBITION; CORTEX; GTP; SENSITIVITY; STRIATUM AB Considerable evidence suggests that signal transduction pathways are targets of lithium (Li) action. A number of investigators have reported that Li attenuates both adenylate cyclase (AC) activity and phosphoinositide (PI) turnover in rodents and in humans, thus "dampening" these systems. We have studied selected components of these second-messenger systems in a series of clinical and preclinical investigations. To overcome confounding effects cf alterations in mood state, we examing AC activity and G-protein ribosylation in peripheral blood cells from 10 healthy volunteers, prior to and following 14 days of Li administration. Basal and postreceptor [cesium fluoride (CsF) or Gpp(NH)p] stimulated AC activity were unaffected in lymphocytes. In contrast, both basal and stimulated AC activity in platelets were significantly augmented, compatible with an attenuation of G(i) function. Ribosylation of platelet G(s) by cholera toxin was unchanged, whereas that of G(i) by pertussis toxin (PT) was increased. Given that undissociated G protein is the preferred substrate for PT, our results suggest that Li interferes with subunit dissociation and the subsequent activation of G(i). To determine if Li has similar effects on G(i) in the central nervous system, we measured extracellular (EC) cyclic adenosine monophosphate (cAMP) in rat brain by in vivo microdialysis, revealing a dose-dependent increase in cAMP by norepinephrine (NE) antagonized by propranolol. Chronic (4-week) Li doubled basal EC cAMP, while decreasing the fractional response to 100-mu-M NE. Thus, using in vivo microdialysis, we observed the reported reduction in NE-stimulated AC activity, but only as a function of elevated basal cAMP. Increased basal AC activity has been observed following chronic Li in both humans and rat tissues but generally has not been considered relevant. The PI generating system is another proposed major target for Li that we have studied using an in vitro cell culture model of peripheral blood cells. Chronic (6-day) exposure of neutrophil-like HL60 cells to 1 mM LiCI did not affect agonist fMet-Leu-Phe (fMLP) induced PI turnover. In contrast, Li attenuated both agonist and phorbol ester stimulated Na+/H+ exchange, suggesting reduced protein kinase C (PKC) function. Western blot analysis revealed altered levels of PKC in both membrane and cytosolic fractions. The functional consequences of these complex effects on the two major signal transduction pathways and their interactions in the intact living organism remain to be elucidated. RP MANJI, HK (reprint author), NIMH,EXPTL THERAPEUT BRANCH,CLIN PHARMACOL SECT,BLDG 10,ROOM 2D46,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Chen, Guang/A-2570-2017 NR 31 TC 31 Z9 31 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1991 VL 27 IS 3 BP 199 EP 208 PG 10 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA GP897 UT WOS:A1991GP89700005 PM 1775589 ER PT J AU BRIDGE, TP HESELTINE, PNR PARKER, ES EATON, EM INGRAHAM, LJ MCGRAIL, ML GOODWIN, FK AF BRIDGE, TP HESELTINE, PNR PARKER, ES EATON, EM INGRAHAM, LJ MCGRAIL, ML GOODWIN, FK TI RESULTS OF EXTENDED PEPTIDE-T ADMINISTRATION IN AIDS AND ARC PATIENTS SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 29TH ANNUAL MEETING OF THE AMERICAN COLLEGE OF NEUROPSYCHOPHARMACOLOGY CY DEC, 1990 CL SAN JUAN, PR SP AMER COLL NEUROPSYCHOPHARM ID HUMAN IMMUNODEFICIENCY VIRUS; ENVELOPE PROTEIN; RECEPTOR-BINDING; HIV; OCTAPEPTIDES; INFECTIVITY AB We report here the extended Phase I testing of d-ala-Peptide-T-amide (Peptide) in open trial. The drug was given intravenously in doses ranging from 0.1 to 3.2 mg/kg/day to 14 acquired immunodeficiency syndrome (AIDS) and AIDS-related complex (ARC) patients for 12 weeks. Following a 4-week off-drug period, the first 6 patients finishing the intravenous testing were continued on intranasal drug, 25 mg/day, for 8 weeks. Control subjects were tested on the same neuropsychologic tests, but did not receive drug. Minimal evidence of toxicity was found. Performance increments in cognitive and neuromotor function were observed in patients with moderate neuropsychologic impairment compared with controls. Changes in constitutional symptoms included weight gain averaging 2 kg and reported improved sense of well-being. The latter findings were independent of variation in cognitive and neuromotor function. Measures of immunologic function and antiviral activity did not change significantly during the study. These data provide a scientific rationale for Phase II testing of Peptide T in human immunodeficiency virus-1 (HIV-1) patients focusing on neuropsychiatric outcome. C1 CTR NEUROPSYCHOL,IRVINE,CA. UNIV SO CALIF,LOS ANGELES,CA 90089. RP BRIDGE, TP (reprint author), NIMH,INTRAMURAL RES PROGRAM,ROOM 4N224,BLDG 10,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. FU NCRR NIH HHS [RR-43]; PHS HHS [278-88-002] NR 26 TC 19 Z9 19 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1991 VL 27 IS 3 BP 237 EP 245 PG 9 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA GP897 UT WOS:A1991GP89700011 PM 1775594 ER PT J AU MANJI, HK CHEN, G BITRAN, JA POTTER, WZ AF MANJI, HK CHEN, G BITRAN, JA POTTER, WZ TI DOWN-REGULATION OF BETA-RECEPTORS BY DESIPRAMINE INVITRO INVOLVES PKC/PHOSPHOLIPASE-A2 SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 29TH ANNUAL MEETING OF THE AMERICAN COLLEGE OF NEUROPSYCHOPHARMACOLOGY CY DEC, 1990 CL SAN JUAN, PR SP AMER COLL NEUROPSYCHOPHARM ID COUPLED ADENYLATE-CYCLASE; GLIOMA C6 CELLS; ADRENERGIC RECEPTORS; INDUCED DESENSITIZATION; BINDING-SITES; RAT; BRAIN; ANTIDEPRESSANTS; SYSTEM AB Chronic treatment with a number of antidepressants results in a down-regulation and/or a desensitization of rat cortical beta-adrenergic receptors (beta-ARs). Although these effects generally have been attributed to elevations in intrasynaptic norepinephrine via presynaptic mechanisms, the recent demonstration of similar changes in beta-ARs following in vitro incubation of cultured cells with desipramine (DMI) suggests that direct, postsynaptic mechanisms may also be involved. To study these mechanisms, we incubated rat C6 glioma cells with 10-mu-M DMI for 1 or 5 days. DMI produced a significant reduction in beta-AR density following chronic (but not acute) treatment (B(max) control = 1325 +/- 78 fmol/mg; DMI = 1179 +/- 96; p < .05). Interestingly, the beta-AR down-regulation was accompanied by an increase in K(L)/K(H) ratio (ratio of dissociation constants for the low- and high-affinity states of the receptor), suggesting that these drugs may stabilize the high-affinity complex. DMI treatment attenuated the cyclic adenosine monophosphate (cAMP) response to 1-mu-M isoproterenol (control = 540 +/- 82 pmol/mg/15 min; DMI = 335 +/- 64; p < .05), but not to agents acting distal to the receptor (cholera toxin or forskolin). Coincubation of C6 cells with either the phospholipase A2 (PLA2) inhibitor mepacrine or the protein kinase C (PKC) inhibitor H7, during chronic treatment with DMI, blocked the down-regulation of beta-ARs. Incubation of C6 cells with phorbol esters (PKC activators) also down-regulated beta-ARs, effects that were nonadditive with those of DMI. Incubation with H7 alone resulted in an up-regulation of beta-ARs, consistent with a tonic regulatory effect of PKC on beta-ARs. These results suggest that some antidepressants down-regulate beta-ARs, at least in part, by direct postsynaptic mechanisms, which may involve "cross-talk" with PKC +/- arachidonic acid and its metabolites. RP MANJI, HK (reprint author), NIMH,EXPTL THERAPEUT BRANCH,CLIN PHARMACOL SECT,BLDG 10,ROOM 2D46,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Chen, Guang/A-2570-2017 NR 21 TC 9 Z9 10 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1991 VL 27 IS 3 BP 247 EP 253 PG 7 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA GP897 UT WOS:A1991GP89700012 PM 1663633 ER PT J AU RAFFAELE, KC BERARDI, A ASTHANA, S MORRIS, P HAXBY, JV SONCRANT, TT AF RAFFAELE, KC BERARDI, A ASTHANA, S MORRIS, P HAXBY, JV SONCRANT, TT TI EFFECTS OF LONG-TERM CONTINUOUS INFUSION OF THE MUSCARINIC CHOLINERGIC AGONIST ARECOLINE ON VERBAL MEMORY IN DEMENTIA OF THE ALZHEIMER TYPE SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 29TH ANNUAL MEETING OF THE AMERICAN COLLEGE OF NEUROPSYCHOPHARMACOLOGY CY DEC, 1990 CL SAN JUAN, PR SP AMER COLL NEUROPSYCHOPHARM ID ORAL PHYSOSTIGMINE; DISEASE; SYSTEM; LECITHIN; THERAPY; TRIAL AB Alzheimer's disease (AD) is accompanied by depletion of cholinergic markers in the central nervous system. In an attempt to improve cognitive function in AD, arecoline (a muscarinic cholinergic receptor agonist) was given to patients with probable or possible AD in a two-phase design, and verbal memory function was examined. First, escalating doses of arecoline, range .5-40 mg/day, were administered by continuous intravenous infusion over a 2-week period. Based on neuropsychological test performance, an optimal dose of arecoline was identified, and this dose then was infused continuously for 5 days in a double-blind, placebo-controlled, counter-balanced trial. Long-term recall on a selective reminding task was significantly improved (from 11.8 to 20.1 words, p < .05) during dose finding by arecoline at 4 mg/day. Two of eight patients were nonresponders; that is, they showed no improvement at any dose during the dose-finding study. Responders demonstrated significant improvement on total recall (p < .05) during the double-blind study. These results indicate that some patients demonstrate reliable improvements of verbal memory during arecoline treatment. RP RAFFAELE, KC (reprint author), NIA,NEUROSCI LAB,BRAIN AGING & DEMENTIA SECT,BLDG 10,ROOM 6C414,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 29 TC 27 Z9 28 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1991 VL 27 IS 3 BP 315 EP 319 PG 5 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA GP897 UT WOS:A1991GP89700023 PM 1775605 ER PT J AU DAWKINS, K POTTER, WZ AF DAWKINS, K POTTER, WZ TI GENDER DIFFERENCES IN PHARMACOKINETICS AND PHARMACODYNAMICS OF PSYCHOTROPICS - FOCUS ON WOMEN SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article ID DEXAMETHASONE SUPPRESSION TEST; SEX-RELATED DIFFERENCES; H-3 LSD BINDING; DRUG DISPOSITION; M-CHLOROPHENYLPIPERAZINE; ANTIPYRINE DISPOSITION; ENDOGENOUS-DEPRESSION; HEALTHY-SUBJECTS; MENSTRUAL-CYCLE; HUMAN-PLATELETS AB The animal literature provides many instances of gender differences in drug metabolism and/or response. However, there are inherent dangers in trying to generalize findings of either pharmacokinetic or pharmacodynamic gender differences to humans. The literature was searched for references to gender and pharmacokinetics of psychoactive drugs. Interest was primarily in whether sufficient data were available on humans to implicate a gender effect, given the large animal literature. There was a dearth of investigations to look for pertinent differences and many studies that examined gender differences appeared to do so incidentally. Some differences were identified that seemed hormonally influenced and age-related. The most compelling gender differences stem from data on research probes utilized to better understand psychiatric disease processes. The implications for women as psychoactive drug consumers are discussed. RP DAWKINS, K (reprint author), NIMH,EXPTL THERAPEUT BRANCH,CLIN PHARMACOL SECT,BLDG 10,ROOM 2D46,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 68 TC 43 Z9 43 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1991 VL 27 IS 4 BP 417 EP 426 PG 10 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA HJ686 UT WOS:A1991HJ68600004 PM 1813891 ER PT J AU SHOAF, SE LINNOILA, M AF SHOAF, SE LINNOILA, M TI INTERACTION OF ETHANOL AND SMOKING ON THE PHARMACOKINETICS AND PHARMACODYNAMICS OF PSYCHOTROPIC MEDICATIONS SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Review ID MULTIPLE-DOSE PHARMACOKINETICS; CIGARETTE-SMOKING; HEALTHY-VOLUNTEERS; CLINICAL PHARMACOKINETICS; PLASMA-CONCENTRATIONS; REDUCED HALOPERIDOL; DEPRESSIVE PATIENTS; PSYCHOMOTOR-SKILLS; NEUROLEPTIC DRUGS; TOBACCO SMOKING AB Ethanol and smoking are known to affect the pharmacokinetics and pharmacodynamics of many medications. This review focuses on the interactions between ethanol and smoking and psychotropic medications, in particular, antidepressants, anxiolytics, and neuroleptics. A discussion of the various mechanisms by which ethanol and smoking may exert their effects precedes a summary of specific interactions. RP NIAAA, DICBR,CLIN STUDIES LAB,BLDG 10,ROOM 3C-102, 9000 ROCKVILLE PIKE, BETHESDA, MD 20892 USA. NR 132 TC 18 Z9 18 U1 0 U2 0 PU MEDWORKS MEDIA GLOBAL, LLC PI HERMOS BEACH PA 670 FIFTH STREET, STE A, HERMOS BEACH, CA 90254 USA SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1991 VL 27 IS 4 BP 577 EP 594 PG 18 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA HJ686 UT WOS:A1991HJ68600018 PM 1813903 ER PT J AU DEVANE, CL RUDORFER, MV POTTER, WZ AF DEVANE, CL RUDORFER, MV POTTER, WZ TI DOSAGE REGIMEN DESIGN FOR CYCLIC ANTIDEPRESSANTS - A REVIEW OF PHARMACOKINETIC METHODS SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article ID STATE PLASMA-LEVELS; SINGLE-DOSE DATA; STEADY-STATE; DEPRESSED-PATIENTS; ELDERLY PATIENTS; NORTRIPTYLINE; PREDICTION; DESIPRAMINE; IMIPRAMINE; KINETICS AB One of the difficulties in pharmacotherapy of depression is the frequent need to make several dosage titrations before reaching the optimal therapeutic dosage. This problem has led to several experimental approaches for predicting a stable antidepressant maintenance dose from plasma concentration data collected shortly following a test dose. The theoretical basis for these methods is reviewed along with the results of clinical trials. It is concluded that accurate steady-state concentrations of cyclic antidepressants can be predicted from kinetic analysis of plasma concentrations obtained early in therapy. The most applicable drug for prospective dosing methods is nortriptyline with its widely accepted therapeutic plasma concentration range. Other drugs that also have substantial evidence for predicting the optimal dose from an early point in therapy include desipramine and imipramine. Predictive dosing methods are easily applied in the inpatient setting. Multiple data points obtained between 12 and 48 hours after the first dose are likely to give accurate predictions and provide clinically useful information. More widespread application of predictive approaches to dosage regimen design of antidepressant pharmacotherapy may lead to reduced hospitalization and cost savings. C1 NIMH,DIV CLIN RES,ROCKVILLE,MD 20857. NIMH,CLIN PHARMACOL SECT,BETHESDA,MD 20892. RP DEVANE, CL (reprint author), UNIV FLORIDA,BOX J-486,GAINESVILLE,FL 32610, USA. NR 36 TC 6 Z9 6 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1991 VL 27 IS 4 BP 619 EP 631 PG 13 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA HJ686 UT WOS:A1991HJ68600021 PM 1813906 ER PT J AU COLLIVER, JD KOPSTEIN, AN AF COLLIVER, JD KOPSTEIN, AN TI TRENDS IN COCAINE ABUSE REFLECTED IN EMERGENCY ROOM EPISODES REPORTED TO DAWN SO PUBLIC HEALTH REPORTS LA English DT Article AB The National Institute on Drug Abuse supports the Drug Abuse Warning Network (DAWN), a voluntary data collection system through which hospital emergency room (ER) and medical examiner facilities report information on medical crises and deaths related to the use of drugs. This study is based on cocaine-related episodes seen at 453 consistently reporting hospital emergency rooms located primarily in 21 U.S. metropolitan areas. Cocaine-related medical emergencies reported to DAWN increased from 16,033 in the first half of 1987 to 25,607 in the first half of 1989; they decreased to 22,796 in the second half of 1989. In the Boston, Buffalo, Dallas, Detroit, Minneapolis, New York, Newark, Phoenix, and Washington, DC, areas, cocaine-related ER episodes decreased for at least the last two consecutive semiannual periods. Consistent with the prevalence of crack, smoking was the most frequently reported route of administering cocaine. Patients who had smoked the drug generally were younger and less likely to use other drugs in combination than were those who took cocaine by other routes. The proportion of black patients increased from 57 to 63 percent in cocaine-related ER episodes overall, and from 74 to 77 percent in episodes where the drug was smoked. Heroin used in combination with cocaine was reported in 12 to 15 percent of cocaine episodes, and both drugs were injected in 75 to 78 percent of the cases where both were involved, suggesting so-called speedballing. Patients who combined heroin with cocaine were generally older than patients in cocaine episodes overall. C1 NATL INST DRUG ABUSE,DIV EPIDEMIOL & PREVENT RES,ROCKVILLE,MD. RP COLLIVER, JD (reprint author), CSR INC,1400 1 ST NW,SUITE 600,WASHINGTON,DC 20005, USA. FU PHS HHS [271-89-8325] NR 8 TC 45 Z9 45 U1 0 U2 1 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0033-3549 J9 PUBLIC HEALTH REP JI Public Health Rep. PD JAN-FEB PY 1991 VL 106 IS 1 BP 59 EP 68 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EX644 UT WOS:A1991EX64400013 PM 1847529 ER PT B AU HIGGINS, M AF HIGGINS, M BE WEINBAUM, G GILES, RE KRELL, RD TI RISK-FACTORS ASSOCIATED WITH CHRONIC OBSTRUCTIVE LUNG-DISEASE SO PULMONARY EMPHYSEMA: THE RATIONALE FOR THERAPEUTIC INTERVENTION SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON PULMONARY EMPHYSEMA : THE RATIONALE FOR THERAPEUTIC INTERVENTION CY MAY 16-18, 1990 CL LAKE BUENA VISTA, FL SP NEW YORK ACAD SCI, AMER THORAC SOC ID MORTALITY; TECUMSEH; DEFICIENCY; INDEX RP HIGGINS, M (reprint author), NHLBI,BETHESDA,MD 20892, USA. NR 0 TC 42 Z9 45 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-660-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 624 BP 7 EP 17 DI 10.1111/j.1749-6632.1991.tb17001.x PG 11 WC Biochemistry & Molecular Biology; Pathology; Pharmacology & Pharmacy; Physiology; Respiratory System SC Biochemistry & Molecular Biology; Pathology; Pharmacology & Pharmacy; Physiology; Respiratory System GA BT99U UT WOS:A1991BT99U00002 PM 2064251 ER PT J AU WINK, DA DESROSIERS, MF AF WINK, DA DESROSIERS, MF TI UNUSUAL SPIN-TRAP CHEMISTRY FOR THE REACTION OF HYDROXYL RADICAL WITH THE CARCINOGEN N-NITROSODIMETHYLAMINE SO RADIATION PHYSICS AND CHEMISTRY LA English DT Article ID AQUEOUS-SOLUTIONS; NITROSAMINES; DENITROSATION AB The reaction of the potent carcinogen N-nitrosodimethylamine (NDMA) with hydroxyl radical generated via radiolysis was studied using EPR techniques. Attempts to spin trap NDMA radical intermediates with 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS) produced only unusual DBNBS radicals. One of these radicals was shown to be generated by both reaction of DBNBS with nitric oxide, and direct oxidation of DBNBS with an inorganic oxidant (.Br2-). Another DBNBS radical was identified as a sulfite spin adduct resulting from the degradation of DBNBS by a NDMA reactive intermediate. In the absence of DBNBS, hydroxyl radical reaction with NDMA gave the dimethylnitroxide radical. Unexpectedly, addition of DBNBS to a solution containing dimethylnitroxide produced an EPR spectrum nearly identical to that of NDMA solutions with DBNBS added before radiolysis. A proposed mechanism accounting for these observations is presented. C1 NATL INST STAND & TECHNOL,DIV IONIZING RADIAT,GAITHERSBURG,MD 20899. NCI,FREDERICK CANC RES & DEV CTR,COMPARAT CARCINOGENESIS LAB,CHEM SECT,FREDERICK,MD 21702. NR 15 TC 8 Z9 8 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB J9 RADIAT PHYS CHEM JI Radiat. Phys. Chem. PY 1991 VL 38 IS 5 BP 467 EP 472 PG 6 WC Chemistry, Physical; Nuclear Science & Technology; Physics, Atomic, Molecular & Chemical SC Chemistry; Nuclear Science & Technology; Physics GA GK954 UT WOS:A1991GK95400006 ER PT J AU MILLER, DL DOPPMAN, JL AF MILLER, DL DOPPMAN, JL TI PETROSAL SINUS SAMPLING - TECHNIQUE AND RATIONALE SO RADIOLOGY LA English DT Article DE CUSHING SYNDROME; HORMONES; SINUSES, DURAL ID CORTICOTROPIN-RELEASING HORMONE; ACTH-SECRETING MICROADENOMAS; CUSHINGS-SYNDROME; PITUITARY MICROADENOMAS; STIMULATION TEST; DISEASE; LOCALIZATION; LATERALIZATION; DIAGNOSIS; INVITRO C1 GEORGETOWN UNIV,MED CTR,DEPT RADIOL,WASHINGTON,DC 20007. RP MILLER, DL (reprint author), NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT DIAGNOST RADIOL,BLDG 10,BETHESDA,MD 20892, USA. NR 58 TC 83 Z9 87 U1 0 U2 1 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD JAN PY 1991 VL 178 IS 1 BP 37 EP 47 PG 11 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EP117 UT WOS:A1991EP11700009 PM 1845785 ER PT J AU DOPPMAN, JL MILLER, DL CHANG, R SHAWKER, TH GORDEN, P NORTON, JA AF DOPPMAN, JL MILLER, DL CHANG, R SHAWKER, TH GORDEN, P NORTON, JA TI INSULINOMAS - LOCALIZATION WITH SELECTIVE INTRAARTERIAL INJECTION OF CALCIUM SO RADIOLOGY LA English DT Article DE ANGIOGRAPHY, PREOPERATIVE; PANCREAS, NEOPLASMS ID BETA-CELL; SECRETION; PANCREAS; INFUSION AB To facilitate the noninvasive preoperative localization of islet cell tumors less than 15 mm in diameter, the authors examined the use of calcuim as an insulin secretagogue in an arterial stimulation venous sampling (ASVS) technique. In four patients with episodic hypoglycemia, calcium gluconate (0.01-0.025 mEq Ca2+/kg) was injected directly into branches of the celiac plexus (gastroduodenal, splenic, and hepatic arteries) and the superior mesenteric artery. In all patients, serum levels of insulin rose abruptly in blood samples taken from the right hepatic vein 30 and 60 seconds after the infusion of calcium into the artery supplying the tumor; injection into an artery not supplying the tumor did not result in a similar rise. Accurate localization of the insulinomas was verified at surgery in three patients. In the fourth patient, who did not undergo surgery, arteriographic results were positive for insulinoma at the predicted site. On the basis of these results, the authors believe noninvasive ASVS may replace invasive portal venous sampling as the most effective method for the localization of occult insulinomas. C1 NCI,SURG BRANCH,SURG METAB SECT,BETHESDA,MD 20892. GEORGETOWN UNIV,MED CTR,DEPT RADIOL,WASHINGTON,DC 20007. NIDDKD,DIABET BRANCH,BETHESDA,MD. RP DOPPMAN, JL (reprint author), NIH,WARREN G MAGNUSON CLIN CTR,DEPT DIAGNOST RADIOL,BLDG 10,RM IC660,BETHESDA,MD 20892, USA. NR 18 TC 200 Z9 210 U1 0 U2 0 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD JAN PY 1991 VL 178 IS 1 BP 237 EP 241 PG 5 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EP117 UT WOS:A1991EP11700044 PM 1984311 ER PT J AU MILLER, DL AF MILLER, DL TI SELECTIVE CATHETERIZATION OF THE INFERIOR PETROSAL SINUSES - NEW CATHETER DESIGN SO RADIOLOGY LA English DT Article DE CATHETERS AND CATHETERIZATION, TECHNOLOGY; SINUSES, DURAL AB Catheters for selective catheterization of the right and left inferior petrosal sinuses have been developed to replace the complex tip-deflector catheter-guide-wire system currently used. The new catheters are easily formed from commonly available straight catheters with the use of steam. They have been successfully tested in 22 patients; the only complications were minor groin hematomas. C1 GEORGETOWN UNIV,MED CTR,DEPT RADIOL,WASHINGTON,DC 20007. RP MILLER, DL (reprint author), NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT DIAGNOST RADIOL,BLDG 10,BETHESDA,MD 20892, USA. NR 10 TC 6 Z9 6 U1 0 U2 0 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD JAN PY 1991 VL 178 IS 1 BP 281 EP 282 PG 2 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA EP117 UT WOS:A1991EP11700053 PM 1843445 ER PT J AU BARRETT, JC HUFF, J AF BARRETT, JC HUFF, J TI CELLULAR AND MOLECULAR MECHANISMS OF CHEMICALLY-INDUCED RENAL CARCINOGENESIS SO RENAL FAILURE LA English DT Article DE CANCER MECHANISMS; CARCINOGENESIS; CHEMICALS AND CANCER; HYALINE DROPLETS; KIDNEY CANCER RP BARRETT, JC (reprint author), NIEHS,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 25 Z9 25 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0886-022X J9 RENAL FAILURE JI Ren. Fail. PY 1991 VL 13 IS 4 BP 211 EP 225 DI 10.3109/08860229109022157 PG 15 WC Urology & Nephrology SC Urology & Nephrology GA GY889 UT WOS:A1991GY88900001 PM 1780490 ER PT B AU LOPEZ, FJ NEGROVILAR, A AF LOPEZ, FJ NEGROVILAR, A BE NEGROVILAR, A PEREZPALACIOS, G TI NEUROENDOCRINE REGULATION OF PULSATILE PROLACTIN SECRETION SO REPRODUCTION, GROWTH AND DEVELOPMENT SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT SCIENTIFIC MEETING ON REPRODUCTION, GROWTH AND DEVELOPMENT CY 1990 CL ACAPULCO, MEXICO SP ARES SERONO SYMP RP LOPEZ, FJ (reprint author), NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,REPROD NEUROENDOCRINOL SECT,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-650-0 J9 SERONO SYM PY 1991 VL 71 BP 399 EP 408 PG 10 WC Biochemistry & Molecular Biology; Developmental Biology; Endocrinology & Metabolism; Obstetrics & Gynecology; Pediatrics SC Biochemistry & Molecular Biology; Developmental Biology; Endocrinology & Metabolism; Obstetrics & Gynecology; Pediatrics GA BV29N UT WOS:A1991BV29N00040 ER PT J AU SANTTI, R NEWBOLD, RR MCLACHLAN, JA AF SANTTI, R NEWBOLD, RR MCLACHLAN, JA TI ANDROGEN METABOLISM IN CONTROL AND NEONATALLY ESTROGENIZED MALE-MICE SO REPRODUCTIVE TOXICOLOGY LA English DT Article DE ANDROGEN METABOLISM; NEONATAL ESTROGENIZATION; MALE GENITAL TRACT; DIETHYLSTILBESTROL; DES ID BENIGN PROSTATIC HYPERPLASIA; RAT VENTRAL PROSTATE; ACCESSORY SEX-ORGANS; MALE PSEUDOHERMAPHRODITISM; FUNCTIONAL-DEVELOPMENT; AROMATASE-ACTIVITY; PRENATAL EXPOSURE; SEMINAL-VESICLES; TESTOSTERONE; 5-ALPHA-REDUCTASE AB Reduction, oxidation, and aromatization of androgens were studied in the male genital tract of untreated control and neonatally estrogenized mice. This study shows regional differences in 5-alpha-reductase and 17-beta-hydroxysteroid oxidoreductase activities in untreated male genital tract; 3-alpha/3-beta-hydroxysteroid oxidoreductase (3-alpha/3-beta-HSOR) activity varied little between tissues. Neonatal treatment with diethylstilbestrol (DES, 2-mu-g/pup/day on days 1 through 5) caused an alteration in the androgen metabolism of the male genital tract, resulting in apparent decreased net accumulation of dihydrotestosterone (DHT). This developmentally-induced 5-alpha-reductase deficiency may play a role in the long-term inhibitory effects of early estrogenization by DES in the growth and function of male sex accessory glands. No aromatase activity could be demonstrated in the male genital tract of control or neonatally estrogenized mice. C1 NIEHS,DIV INTRAMURAL RES,REPROD & DEV TOXICOL LAB,DEV ENDOCRINOL & PHARMACOL SECT,RES TRIANGLE PK,NC 27709. NR 39 TC 11 Z9 11 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0890-6238 J9 REPROD TOXICOL JI Reprod. Toxicol. PY 1991 VL 5 IS 2 BP 149 EP 155 DI 10.1016/0890-6238(91)90043-F PG 7 WC Reproductive Biology; Toxicology SC Reproductive Biology; Toxicology GA FB805 UT WOS:A1991FB80500008 PM 1807546 ER PT J AU GRAFMAN, J JOHNSON, R SCHEFFERS, M AF GRAFMAN, J JOHNSON, R SCHEFFERS, M TI COGNITIVE AND MOOD-STATE CHANGES IN PATIENTS WITH CHRONIC FATIGUE SYNDROME SO REVIEWS OF INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT CONF ON CONSIDERATIONS IN THE DESIGN OF STUDIES OF CHRONIC FATIGUE SYNDROME CY SEP 15-16, 1988 CL PITTSBURGH, PA SP UNIV PITTSBURGH, NIAID ID EPSTEIN-BARR VIRUS; INFECTIOUS-MONONUCLEOSIS; MYALGIC ENCEPHALOMYELITIS; AFFECTIVE-DISORDERS; CHIEF COMPLAINT; DEPRESSION; ANTIBODIES; ENCEPHALITIS; IMMUNITY; ILLNESS AB In this paper the cognitive and psychiatric impairments associated with chronic fatigue syndrome (CFS) and related disorders are reviewed. It is concluded that while acute mononucleosis and infection with Epstein-Barr virus occasionally result in impaired cognition, such changes have not yet been objectively verified in patients with CFS. However, when patients with CFS are carefully studied, concurrent or premorbid psychiatric disorders are revealed at a greater than chance level. Finally, some suggestions are offered regarding improved neuropsychological assessment of fatigue, concentration, and attention for patients with CFS. The findings to date, while suggesting that psychological predisposition may play a role in the expression of CFS, are still inconclusive regarding the etiology of CFS. RP GRAFMAN, J (reprint author), NINCDS,MED NEUROL BRANCH,COGNIT NEUROSCI SECT,BLDG 10,ROOM 5C422,BETHESDA,MD 20892, USA. NR 64 TC 17 Z9 17 U1 3 U2 3 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0162-0886 J9 REV INFECT DIS PD JAN-FEB PY 1991 VL 13 SU 1 BP S45 EP S52 PG 8 WC Immunology; Microbiology SC Immunology; Microbiology GA EV311 UT WOS:A1991EV31100009 PM 1850543 ER PT J AU SCHLUEDERBERG, A STRAUS, SE GRUFFERMAN, S AF SCHLUEDERBERG, A STRAUS, SE GRUFFERMAN, S TI CONSIDERATIONS IN THE DESIGN OF STUDIES OF CHRONIC FATIGUE SYNDROME - PITTSBURGH, PENNSYLVANIA - 15-16 SEPTEMBER 1988 - INTRODUCTION SO REVIEWS OF INFECTIOUS DISEASES LA English DT Editorial Material C1 NIAID,CLIN INVEST LAB,MED VIROL SECT,BETHESDA,MD 20892. UNIV PITTSBURGH,SCH MED,DEPT CLIN EPIDEMIOL & INFECT DIS,PITTSBURGH,PA 15261. RP SCHLUEDERBERG, A (reprint author), NIAID,CLIN INVEST LAB,DIV MICROBIOL & INFECT DIS,VIROL BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0162-0886 J9 REV INFECT DIS PD JAN-FEB PY 1991 VL 13 SU 1 BP S1 EP S1 PG 1 WC Immunology; Microbiology SC Immunology; Microbiology GA EV311 UT WOS:A1991EV31100001 ER PT J AU STRAUS, SE AF STRAUS, SE TI HISTORY OF CHRONIC FATIGUE SYNDROME SO REVIEWS OF INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT CONF ON CONSIDERATIONS IN THE DESIGN OF STUDIES OF CHRONIC FATIGUE SYNDROME CY SEP 15-16, 1988 CL PITTSBURGH, PA SP UNIV PITTSBURGH, NIAID ID BARR VIRUS-INFECTION; PERSISTENT; ALLERGY; ILLNESS AB Chronic fatigue syndrome is not a new medical condition. For centuries its confusing array of features has been attributed to numerous environmental, metabolic, infectious, immunologic, and psychiatric disturbances. This is a review and critique of many of these alternative diagnoses, sufficient to provide a historical background for current thinking about the disorder. RP STRAUS, SE (reprint author), NIAID,MED VIROL SECT,BLDG 10,ROOM 11N113,BETHESDA,MD 20892, USA. NR 40 TC 41 Z9 41 U1 1 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0162-0886 J9 REV INFECT DIS PD JAN-FEB PY 1991 VL 13 SU 1 BP S2 EP S7 PG 6 WC Immunology; Microbiology SC Immunology; Microbiology GA EV311 UT WOS:A1991EV31100002 PM 2020800 ER PT J AU LAPLANCHE, JL CHATELAIN, J THOMAS, S BROWN, P CATHALA, F AF LAPLANCHE, JL CHATELAIN, J THOMAS, S BROWN, P CATHALA, F TI PRP GENE ANALYSIS IN A TUNISIAN FAMILY WITH CREUTZFELDT-JAKOB DISEASE SO REVUE NEUROLOGIQUE LA French DT Article ID PROTEIN GENE; DNA AB Results of PrP gene analysis in 5 of 9 members from a Jewish Tunisian family with Creutzfeldt-Jakob disease (CJD) showed a mutation at codon 200 involving substitution of lysine (Lys200) for glutamic acid (Glu200). This observation suggests that Lys200 allele probably tracks with CJD in this family and supports the possible genetic basis of the disease in the Mediterranean cluster. A second PrP variant not associated with Lys200 allele involving a short deletion in the coding sequence has also been found in only one subject. C1 NIH,BETHESDA,MD 20892. RP LAPLANCHE, JL (reprint author), HOP ST LOUIS,1 AVE C VELLEFAUX,F-75010 PARIS,FRANCE. RI LAPLANCHE, Jean-Louis/S-8707-2016 NR 9 TC 10 Z9 11 U1 0 U2 0 PU MASSON EDITEUR PI PARIS 06 PA 120 BLVD SAINT-GERMAIN, 75280 PARIS 06, FRANCE SN 0035-3787 J9 REV NEUROL JI Rev. Neurol. PY 1991 VL 147 IS 12 BP 825 EP 827 PG 3 WC Clinical Neurology SC Neurosciences & Neurology GA GW048 UT WOS:A1991GW04800006 PM 1780610 ER PT J AU BALOW, JE AF BALOW, JE TI KIDNEY-DISEASE IN SYSTEMIC LUPUS-ERYTHEMATOSUS SO RHEUMATOLOGY INTERNATIONAL LA English DT Article; Proceedings Paper CT 1ST EUROPEAN CONF ON SYSTEMIC LUPUS ERYTHEMATOSUS CY NOV 15-17, 1990 CL AMSTERDAM, NETHERLANDS SP DUTCH LUPUS FDN DE SYSTEMIC LUPUS ERYTHEMATOSUS; LUPUS NEPHRITIS; IMMUNOSUPPRESSIVE DRUGS; CYCLOPHOSPHAMIDE; PREDNISONE ID CONTROLLED TRIAL; INTRAVENOUS CYCLOPHOSPHAMIDE; NEPHRITIS; THERAPY; PREDNISONE; BIOPSY AB Glomerulonephritis is a major determinant of outcome in patients with systemic lupus erythematosus. Persistently active lupus nephritis imposes serious threats of end-stage renal failure and cardiovascular morbidity. Sustained corticosteroid treatment has been characterized as having an uncertain net benefit on the control of lupus nephritis, mainly because these drugs have relatively weak efficacy and they have been shown to confer their own set of cardiovascular risk factors. Controlled trials of corticosteroids, azathioprine and cyclophosphamide have demonstrated that the best control of clinical activity of proliferative lupus nephritis is attained with cyclophosphamide. To date, intermittent pulse cyclophosphamide treatment has produced the most favorable balance of efficacy and toxicity in patients with lupus nephritis. RP BALOW, JE (reprint author), NIH,CTR CLIN,9N-222,BETHESDA,MD 20892, USA. NR 24 TC 6 Z9 6 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0172-8172 J9 RHEUMATOL INT JI Rheumatol. Int. PY 1991 VL 11 IS 3 BP 113 EP 115 DI 10.1007/BF00304498 PG 3 WC Rheumatology SC Rheumatology GA GJ663 UT WOS:A1991GJ66300006 PM 1754812 ER PT B AU MARX, SJ AF MARX, SJ BE GLORIEUX, FH TI 1,25-DIHYDROXYVITAMIN-D3 RECEPTORS AND RESISTANCE - IMPLICATIONS IN RICKETS, OSTEOMALACIA, AND OTHER CONDITIONS SO RICKETS SE NESTLE NUTRITION WORKSHOP SERIES LA English DT Proceedings Paper CT 21ST NESTLE NUTRITION WORKSHOP : RICKETS CY DEC 05-08, 1988 CL BUENOS AIRES, ARGENTINA SP NESTLE NUTRIT RP MARX, SJ (reprint author), NIDDKD,MINERAL METAB SECT,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-809-0 J9 NESTLE NUTR WORKS SE PY 1991 VL 21 BP 167 EP 184 PG 18 WC Biochemistry & Molecular Biology; Public, Environmental & Occupational Health; Nutrition & Dietetics; Pathology SC Biochemistry & Molecular Biology; Public, Environmental & Occupational Health; Nutrition & Dietetics; Pathology GA BT96F UT WOS:A1991BT96F00012 ER PT B AU MURRAY, EA AF MURRAY, EA BE HOLSTEGE, G TI CONTRIBUTIONS OF THE AMYGDALAR COMPLEX TO BEHAVIOR IN MACAQUE MONKEYS SO ROLE OF THE FOREBRAIN IN SENSATION AND BEHAVIOR SE PROGRESS IN BRAIN RESEARCH LA English DT Proceedings Paper CT SYMP ON THE ROLE OF THE FOREBRAIN IN SENSATION AND BEHAVIOR CY MAY 26-27, 1989 CL NASA, AMES RES CTR, MOFFETT FIELD, CA HO NASA, AMES RES CTR ID STIMULUS-REWARD ASSOCIATIONS; VISUAL CORTICAL AREAS; RHESUS-MONKEY; SUBSTANTIA INNOMINATA; LATERAL HYPOTHALAMUS; AFFERENT CONNECTIONS; MACACA-FASCICULARIS; BASAL FOREBRAIN; THALAMIC NUCLEUS; MEMORY RP MURRAY, EA (reprint author), NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892, USA. NR 0 TC 2 Z9 2 U1 0 U2 1 PU ELSEVIER SCIENCE PUBL B V PI AMSTERDAM PA AMSTERDAM BN 0-444-81181-8 J9 PROG BRAIN RES PY 1991 VL 87 BP 167 EP 180 PG 14 WC Anatomy & Morphology; Behavioral Sciences; Neurosciences SC Anatomy & Morphology; Behavioral Sciences; Neurosciences & Neurology GA BV12J UT WOS:A1991BV12J00008 ER PT J AU BURGDORFER, W SCHWAN, TG AF BURGDORFER, W SCHWAN, TG TI LYME-BORRELIOSIS - A RELAPSING FEVER-LIKE DISEASE SO SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 4TH INTERNATIONAL CONF ON LYME BORRELIOSIS CY JUN 18-21, 1990 CL STOCKHOLM, SWEDEN ID TICKS; SPIROCHETE; MICE AB To determine by xenodiagnosis length and concentrations of spirochetemias produced by Borrelia burgdorferi in white-footed mice (Peromyscus leucopus), laboratory reared mice were inoculated with either spirochete-containing tick suspensions or BSK II spirochete culture and were exposed for as long as three months to larval Ixodes dammini. Upon development to the nymphal stage, ticks were evaluated for spirochetal infections by direct immunofluorescence. All mice were found to circulate spirochetes for at least three months in concentrations sufficient to infect ticks. The percentage of infected ticks alternated from low to high, suggesting occurrence of episodes of mild and heavy spirochetemias. The results suggest that B. burgdorferi in its animal hosts and possibly also in humans causes prolonged spirochetemias characterized by episodes of alternating high and low concentrations of spirochetes as reflected by similar percentages of infected ticks. The long persistence of spirochetes in the peripheral blood stream and the cyclical form of Lyme borreliosis appear to be related, as in relapsing fevers, to the capacity of B. burgdorferi to undergo antigenic variations. RP BURGDORFER, W (reprint author), NIAID,ROCKY MT LABS,VECTORS & PATHOGENS LAB,HAMILTON,MT 59840, USA. NR 11 TC 0 Z9 0 U1 1 U2 1 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0036-5548 J9 SCAND J INFECT DIS JI Scand. J. Infect. Dis. PY 1991 SU 77 BP 17 EP 22 PG 6 WC Infectious Diseases SC Infectious Diseases GA GD437 UT WOS:A1991GD43700004 ER PT J AU SCHWAN, TG SIMPSON, WJ AF SCHWAN, TG SIMPSON, WJ TI FACTORS INFLUENCING THE ANTIGENIC REACTIVITY OF BORRELIA BURGDORFERI, THE LYME-DISEASE SPIROCHETE SO SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 4TH INTERNATIONAL CONF ON LYME BORRELIOSIS CY JUN 18-21, 1990 CL STOCKHOLM, SWEDEN ID MICE PEROMYSCUS-LEUCOPUS; PROTEIN; CULTIVATION AB The immunologic reactivity of some antigens of low passage Borrelia burgdorferi varied with the temperature at which it was cultivated in vitro, with long-term cultivation in vitro, and following passage in white-footed mice, Peromyscus leucopus. The low passaged uncloned strain Sh-2-82 had either more antigens or antigens which were more immunoreactive when grown at various temperatures from 28-39-degrees-C. The influence of temperatures was less evident, however with a higher passage of the same strain. Immune sera from white-footed mice experimentally infected with the spirochete were more reactive with antigens of the inoculum than with antigens of spirochetes reisolated later. The antigenicity of the Lyme borreliosis spirochete is becoming more complex and this may relate to the complex life cycle that includes tick and mammalian hosts. RP SCHWAN, TG (reprint author), NIAID,ROCKY MT LABS,VECTORS & PATHOGENS LAB,ARTHROPOD BORNE DIS SECT,HAMILTON,MT 59840, USA. NR 16 TC 0 Z9 0 U1 1 U2 1 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0036-5548 J9 SCAND J INFECT DIS JI Scand. J. Infect. Dis. PY 1991 SU 77 BP 94 EP 101 PG 8 WC Infectious Diseases SC Infectious Diseases GA GD437 UT WOS:A1991GD43700016 ER PT J AU BERGSTROM, S BARBOUR, AG GARON, CF HINDERSSON, P GIRONS, IS SCHWAN, TG AF BERGSTROM, S BARBOUR, AG GARON, CF HINDERSSON, P GIRONS, IS SCHWAN, TG TI GENETICS OF BORRELIA BURGDORFERI SO SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES LA English DT Editorial Material ID LYME-DISEASE SPIROCHETE; DNA; TELOMERES; PLASMIDS; PROTEINS; SEQUENCE; HERMSII; CLONING; GENOME; AGENT AB Structural analysis by electronmicroscopy and pulsed field agarose gel electrophoresis of the genome of Borrelia burgdorferi has revealed a 1000 kilobase (kb) chromosome and a unique type of extra chromosomal linear DNA, as well as supercoiled circular DNA. The plasmid DNA ranged in size from 1.5 to 50 kb. Gene mapping of the ospA and ospB genes have placed them on the 49 kb linear plasmid molecule. The osp operon of B. burgdorferi has been extensively characterized at the molecular level. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MICROBIOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. NIAID,ROCKY MT LABS,VECTORS & PATHOGENS LAB,ARTHROPODE BORNE DIS SECT,HAMILTON,MT 59840. UNIV COPENHAGEN,INST MED MICROBIOL,DK-2100 COPENHAGEN,DENMARK. INST PASTEUR,LEPTOSPIRA LAB,F-75724 PARIS 15,FRANCE. RP BERGSTROM, S (reprint author), UMEA UNIV,DEPT MICROBIOL,S-90187 UMEA,SWEDEN. RI Barbour, Alan/B-3160-2009 OI Barbour, Alan/0000-0002-0719-5248 NR 21 TC 1 Z9 1 U1 0 U2 1 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0036-5548 J9 SCAND J INFECT DIS JI Scand. J. Infect. Dis. PY 1991 SU 77 BP 102 EP 107 PG 6 WC Infectious Diseases SC Infectious Diseases GA GD437 UT WOS:A1991GD43700017 ER PT J AU STIERNSTEDT, G DATTWYLER, R DURAY, PH HANSEN, K JIROUS, J JOHNSON, RC KARLSSON, M PREACMURSIC, V SCHWAN, TG AF STIERNSTEDT, G DATTWYLER, R DURAY, PH HANSEN, K JIROUS, J JOHNSON, RC KARLSSON, M PREACMURSIC, V SCHWAN, TG TI DIAGNOSTIC-TESTS IN LYME-BORRELIOSIS SO SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES LA English DT Editorial Material ID LINKED IMMUNOSORBENT-ASSAY; DISEASE SPIROCHETE; BURGDORFERI; FLAGELLUM; SERODIAGNOSIS; ANTIGENS C1 SUNY STONY BROOK,DEPT MED,STONY BROOK,NY 11794. FOX CHASE CANC INST,DEPT PATHOL,PHILADELPHIA,PA 19111. STATENS SERUM INST,DK-2300 COPENHAGEN,DENMARK. INST HYG & EPIDEMIOL,CS-10042 PRAGUE 10,CZECHOSLOVAKIA. UNIV MINNESOTA,DEPT MICROBIOL,MINNEAPOLIS,MN 55455. PETTENKOFER INST,MUNICH,GERMANY. NIH,ROCKY MT LABS,HAMILTON,MT. RP STIERNSTEDT, G (reprint author), DANDERYD HOSP,DEPT INFECT DIS,STOCKHOLM,SWEDEN. OI Dattwyler, Raymond/0000-0002-1983-1301 NR 14 TC 0 Z9 0 U1 0 U2 0 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0036-5548 J9 SCAND J INFECT DIS JI Scand. J. Infect. Dis. PY 1991 SU 77 BP 136 EP 142 PG 7 WC Infectious Diseases SC Infectious Diseases GA GD437 UT WOS:A1991GD43700020 ER PT J AU LINOS, A KAKLAMANIS, E KONTOMERKOS, A KOUMANTAKI, Y GAZI, S VAIOPOULOS, G TSOKOS, GC KAKLAMANIS, P AF LINOS, A KAKLAMANIS, E KONTOMERKOS, A KOUMANTAKI, Y GAZI, S VAIOPOULOS, G TSOKOS, GC KAKLAMANIS, P TI THE EFFECT OF OLIVE OIL AND FISH CONSUMPTION ON RHEUMATOID-ARTHRITIS - A CASE CONTROL STUDY SO SCANDINAVIAN JOURNAL OF RHEUMATOLOGY LA English DT Article DE OLIVE OIL AND RHEUMATOID ARTHRITIS; DIETARY FACTORS AND RHEUMATOID ARTHRITIS ID FATTY-ACIDS; SUPPLEMENTATION; PATHOGENESIS; GENERATION AB In an interview based, case control study of Rheumatoid Arthritis (RA) 168 cases and 137 controls were included. Patients and controls were interviewed with regard to a variety of socioeconomic, medical and dietary factors. During univariate analysis it was found that RA cases consumed significantly less olive oil and fish and adhered more rarely to the dietary restrictions traditional in Orthodox lent than controls. Applying multiple logistic analysis though (by which several variables were controlled for), only the association with olive oil consumption and lent adherence remained significant. More specifically; an increase in olive oil consumption by two times per week, resulted in a Relative Risk (RR) for development of RA of 0.49, whereas adherence to lent during the 27 weeks per year prescribed by the Orthodox Church, resulted in a RR of 0.33. We conclude that olive oil consumption and adherence to Orthodox lent may have a protective effect on the development and/or the severity of RA. This is a hypothesis generated by the present study that needs verification. C1 ATHENS MED SCH,DEPT HYG & EPIDEMIOL,ATHENS,GREECE. ATHENS MED SCH,DEPT INTERNAL MED 1,ATHENS,GREECE. GEN HOSP ATHENS,RHEUMATOL CLIN,ATHENS,GREECE. NIH,BETHESDA,MD 20892. NR 29 TC 88 Z9 88 U1 0 U2 2 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0300-9742 J9 SCAND J RHEUMATOL JI Scand. J. Rheumatol. PY 1991 VL 20 IS 6 BP 419 EP 426 PG 8 WC Rheumatology SC Rheumatology GA GY254 UT WOS:A1991GY25400006 PM 1771399 ER PT J AU TORREY, EF AF TORREY, EF TI A VIRAL-ANATOMICAL EXPLANATION OF SCHIZOPHRENIA SO SCHIZOPHRENIA BULLETIN LA English DT Editorial Material ID PARAHIPPOCAMPAL GYRUS; LIMBIC SYSTEM; PATHOLOGY; DISORDER; SEASON; BIRTH AB Recent neuropathological and neuroradiological studies of schizophrenia have pointed to the medial temporal cortex, especially the hippocampus, parahippocampal gyrus, and amygdala, as the areas primarily affected by this disease. Localization of the disease process to these structures may be explained anatomically because they are immediately contiguous to the foramen rotundum. Some viruses are known to ascend the trigeminal nerve and enter the cranial cavity through the foramen rotundum. They might latently infect the medial temporal cortex and be reactivated in early adulthood, producing the symptoms of schizophrenia. The distance from the nasal mucosa to the medial temporal cortex is less than 2 cm in infants. An anatomical explanation of schizophrenia could account for the seasonality of schizophrenic births, the observed excess birth trauma in schizophrenic individuals, the clinical aspects of schizophrenia, such as auditory hallucinations, and the genetic component of the disease. RP TORREY, EF (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,HOSP NEUROPSYCHIAT RES,TWIN STUDY UNIT,WASHINGTON,DC 20032, USA. NR 24 TC 34 Z9 34 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1991 VL 17 IS 1 BP 15 EP 18 PG 4 WC Psychiatry SC Psychiatry GA FC445 UT WOS:A1991FC44500003 PM 2047784 ER PT J AU KEITH, SJ MATTHEWS, SM AF KEITH, SJ MATTHEWS, SM TI THE DIAGNOSIS OF SCHIZOPHRENIA - A REVIEW OF ONSET AND DURATION ISSUES SO SCHIZOPHRENIA BULLETIN LA English DT Article ID TERM FOLLOW-UP; SEVERE MENTAL-ILLNESS; LONG-TERM; DSM-III; PREDICTIVE-VALIDITY; PROGNOSIS; SYMPTOMS; CRITERIA; RELAPSE; METHODOLOGY AB The diagnosis of schizophrenia remains a topic of continuing dialogue both within the United States and internationally, as witnessed by the numerous revisions to the Diagnostic and Statistical Manual (i.e., DSM-I, DSM-II, DSM-III, and DSM-III-R) and the International Classification of Diseases (i.e., ICD-9, and ICD-10). At issue is how best to characterize patients suffering the debilitating symptoms and chronicity associated with the disease and, at the same time, arrive at a diagnosis that has specific clinical utility and can be reliably assessed. The purpose of the following report is to review three issues associated with the diagnosis of schizophrenia: the role of prodromal and residual symptoms, the duration of psychotic symptoms, and the 6-month versus 1-month time criterion. Our general recommendation, based on extant studies providing data related to various facets of the issues under debate, is to use diagnostic criteria that are consistent with the international diagnostic system. However, final decisions will depend on the outcome of the ongoing DSM-IV field trials. RP KEITH, SJ (reprint author), NIMH,DIV CLIN RES,RM 17-99,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. NR 61 TC 47 Z9 48 U1 1 U2 1 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1991 VL 17 IS 1 BP 51 EP 67 PG 17 WC Psychiatry SC Psychiatry GA FC445 UT WOS:A1991FC44500007 PM 2047789 ER PT J AU CHRISTISON, GW KIRCH, DG WYATT, RJ AF CHRISTISON, GW KIRCH, DG WYATT, RJ TI WHEN SYMPTOMS PERSIST - CHOOSING AMONG ALTERNATIVE SOMATIC TREATMENTS FOR SCHIZOPHRENIA SO SCHIZOPHRENIA BULLETIN LA English DT Article ID DOUBLE-BLIND TRIAL; PLACEBO-CONTROLLED TRIAL; LITHIUM-CARBONATE; L-DOPA; RATING-SCALE; DIAGNOSTIC CRITERIA; AFFECTIVE DISORDERS; NEUROLEPTIC DRUGS; PROPRANOLOL; CHLORPROMAZINE AB Many patients with schizophrenia continue to have significant disabling symptoms despite adequate trials of different types and doses of traditional neuroleptics. Clinicians treating these neuroleptic-resistant patients must look to other treatments in the hope of providing some relief. The literature on many of the alternative treatments is too scanty for firm conclusions. We offer criteria for deciding which treatments may warrant consideration. We review the evidence for the eight treatments we found to meet these criteria and discuss clinical points salient to their use in this population. Although not always conclusive, the data do offer clues for treatment guidelines and an approach to choosing among the available treatments is suggested. C1 LOMA LINDA BEHAV MED CTR HOSP,RES,LOMA LINDA,CA. NIMH,DIV CLIN RES,ROCKVILLE,MD 20857. NIMH,NEUROPSYCHIAT BRANCH,INTRAMURAL RES PROGRAM,WASHINGTON,DC 20032. NR 154 TC 129 Z9 129 U1 1 U2 3 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1991 VL 17 IS 2 BP 217 EP 245 PG 29 WC Psychiatry SC Psychiatry GA FR480 UT WOS:A1991FR48000006 PM 1679252 ER PT J AU WYATT, RJ AF WYATT, RJ TI NEUROLEPTICS AND THE NATURAL COURSE OF SCHIZOPHRENIA SO SCHIZOPHRENIA BULLETIN LA English DT Article ID 2-YEAR FOLLOW-UP; MAINTENANCE; ILLNESS; THERAPY; TRIAL; DRUGS; OUTPATIENTS; MEDICATION; DIAGNOSIS; DURATION AB To determine if neuroleptic treatment changes the natural course of schizophrenia, 22 studies were reviewed in which relatively similar patients were or were not given neuroleptics at specific times during the course of their illnesses. Nineteen of the studies were from first- or predominately first-break populations. While there was little consensus among the authors of the studies reviewed, a reanalysis of the data indicates that early intervention with neuroleptics in first-break schizophrenic patients increases the likelihood of an improved long-term course. This finding is similar to that of earlier investigators who indicated there was a decrease in patients with the more severe forms of the illness following the introduction of convulsive therapies. Furthermore, there is evidence that stable schizophrenic patients whose neuroleptics are discontinued and have relapses may have a difficult time returning to their previous level of function. The findings described in this paper have implications for both the treatment of schizophrenia and for understanding the pathophysiological processes that determine the course of the disorder. RP WYATT, RJ (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,NEUROPSYCHIAT BRANCH,2700 MARTIN LUTHER KING JR AVE SE,WASHINGTON,DC 20032, USA. NR 85 TC 497 Z9 506 U1 4 U2 12 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1991 VL 17 IS 2 BP 325 EP 351 PG 27 WC Psychiatry SC Psychiatry GA FR480 UT WOS:A1991FR48000011 PM 1679255 ER PT J AU RUPP, A AF RUPP, A TI UNDERINSURANCE FOR SEVERE MENTAL-ILLNESS SO SCHIZOPHRENIA BULLETIN LA English DT Article ID HEALTH-CARE; ECONOMIC COSTS; SCHIZOPHRENIA; COVERAGE; SERVICES AB Mental health care for the severely mentally ill in the United States is financed by a combination of public and private funds. Both public and private health insurance programs handle mental illness differently than they do other illnesses. This article documents uninsurance and underinsurance for severe mental illness in the United States based on studies conducted during the last decade. The relationship between private insurance and public assistance is analyzed, and major arguments around equality in insurance coverage for severe mental illness are examined. Alternatives for reducing uninsurance and underinsurance for severe mental illness in order to avoid undertreatment are discussed. RP RUPP, A (reprint author), NIMH,MENTAL HLTH ECON RES PROGRAM,5600 FISHERS LN,RM 18C 14,ROCKVILLE,MD 20857, USA. NR 26 TC 15 Z9 15 U1 3 U2 3 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1991 VL 17 IS 3 BP 401 EP 405 PG 5 WC Psychiatry SC Psychiatry GA GF467 UT WOS:A1991GF46700008 PM 1947864 ER PT J AU BARTKO, JJ AF BARTKO, JJ TI MEASUREMENT AND RELIABILITY - STATISTICAL THINKING CONSIDERATIONS SO SCHIZOPHRENIA BULLETIN LA English DT Article ID PSYCHIATRIC-DIAGNOSIS; AGREEMENT AB Reliability is defined as the degree to which multiple assessments of a subject agree (reproducibility). There is increasing awareness among researchers that the two most appropriate measures of reliability are the intraclass correlation coefficient and kappa. However, unacceptable statistical measures of reliability such as chi-square, percent agreement, product moment correlation, as well as any measure of association and Yule's Y still appear in the literature. There are costs associated with improper measurements, unreliable diagnostic systems, inappropriate statistics and measures of reliability, and poor quality research. Costs are incurred when misleading information directs resources and talents into nonproductive avenues of research. The consequences of unreliable measurements and diagnosis are illustrated with some studies of schizophrenia. RP BARTKO, JJ (reprint author), NIMH,NIH CAMPUS,BLDG 10,RM 3N-204,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 19 TC 177 Z9 177 U1 1 U2 3 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1991 VL 17 IS 3 BP 483 EP 489 PG 7 WC Psychiatry SC Psychiatry GA GF467 UT WOS:A1991GF46700018 PM 1947873 ER PT J AU KIRCH, DG WAGMAN, AMI GOLDMANRAKIC, PS AF KIRCH, DG WAGMAN, AMI GOLDMANRAKIC, PS TI THE ACQUISITION AND USE OF HUMAN BRAIN-TISSUE IN NEUROPSYCHIATRIC RESEARCH - COMMENTARY SO SCHIZOPHRENIA BULLETIN LA English DT Article ID SCHIZOPHRENIA AB Witelson and McCulloch (1991) report on the establishment of a collection of normal human brain tissue that was secured through a unique method of premortem and postmortem assessments. Their work highlights the growing need in neuroscientific research for carefully characterized collections of human brain tissue from both normal control subjects and patients with specific neuropsychiatric disorders. Brain tissue of this type, however, has not been readily available. Patients with mental disorders may not be competent to consent to a postmortem brain donation; other obstacles include various socioeconomic and legal impediments to autopsies. In addition, a number of methodologic issues exist regarding human brain tissue collections, including the problem of standardized postmortem diagnostic assessment and difficulty in establishing uniform procedures for processing formalin-fixed and frozen tissue. Various proposals to enhance brain-tissue collections are discussed. These include the establishment of networks for tissue donation and use, the linking of ultimate postmortem brain tissue collection to prospective clinical studies, and promulgation of standardized procedures for methods of postmortem diagnosis and tissue handling. C1 NIMH,SCHIZOPHRENIA RES BRANCH,ROCKVILLE,MD 20857. YALE UNIV,SCH MED,NEW HAVEN,CT 06510. RP KIRCH, DG (reprint author), NIMH,DIV CLIN RES,5600 FISHERS LN,RM 10-105,ROCKVILLE,MD 20857, USA. NR 10 TC 3 Z9 3 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1991 VL 17 IS 4 BP 593 EP 596 PG 4 WC Psychiatry SC Psychiatry GA GX260 UT WOS:A1991GX26000006 PM 1805352 ER PT J AU TANAKA, T HOCKMAN, S MOOS, M TAIRA, M MEACCI, E MURASHIMA, S MANGANIELLO, VC AF TANAKA, T HOCKMAN, S MOOS, M TAIRA, M MEACCI, E MURASHIMA, S MANGANIELLO, VC TI COMPARISON OF PUTATIVE CGMP-BINDING REGIONS IN BOVINE BRAIN AND CARDIAC CGMP-STIMULATED PHOSPHODIESTERASES SO SECOND MESSENGERS AND PHOSPHOPROTEINS LA English DT Article ID CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE; CALF LIVER; PURIFICATION; SPECIFICITY AB After photolabelling of purified bovine brain particulate cGMP stimulated phosphodiesterase (PDE) with [P-32]cGMP, incubation with V8 Endoproteinase Glu-C produced several fragments; most of the [P-32] was associated with smaller fragments (almost-equal-to 12-14 kDa), and some occasionally with larger fragments (almost-equal-to 55-57 kDa). Partial amino acid sequences were determined for the smaller photolabelled fragments and other peptides. On Western immunoblots, affinity-purified antibodies against a synthetic peptide with a sequence matching part of that of the almost-equal-to 12-14 kDa photolabelled material reacted with intact PDE and the almost-equal-to 12-14 kDa and almost-equal-to 55-57 kDa fragments. Several partial cDNA clones encoding the cGMP-stimulated PDE were isolated from a Lambda Zap II bovine brain cDNA library. Deduced amino acid sequence from one cDNA clone, lambda cGS 3-1, as well as the partial sequence of the almost-equal-to 12-14 kDa and other fragments, exhibited considerable identity with amino acid residues 311-921 of the cardiac cGMP-stimulated PDE (Trong et al., 1990), including the putative cGMP-binding domain (Charbonneau et al., 1990). These results further define this cGMP-binding domain and suggest that different cGMP-stimulated PDEs will exhibit considerable homology, at least in their cGMP-binding region(s) and catalytic domains. C1 NHLBI,CELLULAR METAB LAB,BETHESDA,MD 20892. NIH,CTR BIOL EVALUAT & RES,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. RI Moos, Malcolm/F-3673-2011 OI Moos, Malcolm/0000-0002-9575-9938 NR 18 TC 17 Z9 17 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0895-7479 J9 SEC MESS PHOSPHOPROT JI Second Messeng. Phosphoprot. PY 1991 VL 13 IS 2-3 BP 87 EP 98 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GF248 UT WOS:A1991GF24800001 PM 1659635 ER PT B AU KIRK, KL AF KIRK, KL BE WELCH, JT TI FLUORINE-SUBSTITUTED NEUROACTIVE AMINES SO SELECTIVE FLUORINATION IN ORGANIC AND BIOORGANIC CHEMISTRY SE ACS SYMPOSIUM SERIES LA English DT Review CT SYMP AT THE 199TH NATIONAL MEETING OF THE AMERICAN CHEMICAL SOC : SELECTIVE FLUORINATION IN ORGANIC AND BIOORGANIC CHEMISTRY CY APR 22-27, 1990 CL BOSTON, MA SP AMER CHEM SOC, DIV FLUORINE CHEM ID BETA-ADRENOCEPTOR ACTIVITIES; DIAZONIUM SALTS; ADRENERGIC-RECEPTORS; RING-FLUORINATION; RAT-BRAIN; PHOTOCHEMISTRY; DOPAMINE; AGENTS; NOREPINEPHRINE; DERIVATIVES RP KIRK, KL (reprint author), NATL INST DIABET & DIGEST & KIDNEY DIS,BIOORGAN CHEM LAB,BETHESDA,MD 20892, USA. NR 0 TC 21 Z9 21 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA WASHINGTON BN 0-8412-1948-6 J9 ACS SYM SER PY 1991 VL 456 BP 136 EP 155 PG 20 WC Chemistry, Organic SC Chemistry GA BS80A UT WOS:A1991BS80A00009 ER PT J AU MASOLIVER, J WEISS, GH AF MASOLIVER, J WEISS, GH TI TRANSPORT-EQUATIONS IN CHROMATOGRAPHY WITH A FINITE SPEED OF SIGNAL PROPAGATION SO SEPARATION SCIENCE AND TECHNOLOGY LA English DT Article AB It is known that the diffusion equation used to model transport in a large variety of chromatographic techniques has an infinite speed of signal propagation, i.e., if c(x,t) is the concentration at time t, then c(x,t) > 0 for any t > 0. We generalize and solve the telegraph equation, which is known to have a finite speed of signal propagation, to allow for asymmetric convection, as is appropriate for the theory of chromatographic processes. We derive the telegraph equation from a continuous time random walk picture and examine two sources of convection, an asymmetry in sojourn times in states in which diffusing particles move in one direction or the other, and a corresponding asymmetry in the velocities. C1 NIH,BETHESDA,MD 20892. RP MASOLIVER, J (reprint author), UNIV BARCELONA,DEPT FIS FONAMENTAL,DIAGONAL 647,E-08028 BARCELONA,SPAIN. RI Masoliver, Jaume/F-7198-2016 OI Masoliver, Jaume/0000-0002-5810-879X NR 10 TC 8 Z9 8 U1 0 U2 2 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0149-6395 J9 SEPAR SCI TECHNOL JI Sep. Sci. Technol. PY 1991 VL 26 IS 2 BP 279 EP 289 DI 10.1080/01496399108050472 PG 11 WC Chemistry, Multidisciplinary; Engineering, Chemical SC Chemistry; Engineering GA FH950 UT WOS:A1991FH95000010 ER PT J AU WASSERHEIT, JN AF WASSERHEIT, JN TI PELVIC INFLAMMATORY DISEASE - RESEARCH DIRECTIONS IN THE 1990S SO SEXUALLY TRANSMITTED DISEASES LA English DT Review ID CHLAMYDIA-TRACHOMATIS INFECTION; SEXUALLY-TRANSMITTED DISEASES; GENITAL-TRACT INFECTION; FALLOPIAN-TUBE MUCOSA; BARRIER-METHOD CONTRACEPTIVES; HUMAN IMMUNODEFICIENCY VIRUS; FAMILY-PLANNING CLINICS; POSSIBLE RISK FACTOR; FIELD FOLLOW-UP; NEISSERIA-GONORRHOEAE RP WASSERHEIT, JN (reprint author), NIAID, DEPT HLTH & HUMAN SERV, SEXUALL TRANSMITTED DIS BRANCH, BETHESDA, MD 20892 USA. NR 192 TC 5 Z9 5 U1 1 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0148-5717 J9 SEX TRANSM DIS JI Sex. Transm. Dis. PD JAN-MAR PY 1991 VL 18 IS 1 BP 46 EP 64 PG 19 WC Infectious Diseases SC Infectious Diseases GA EZ356 UT WOS:A1991EZ35600011 ER PT J AU PAUL, WE AF PAUL, WE TI INTERLEUKIN-4 PRODUCTION BY FC-EPSILON-R+-CELLS SO SKIN PHARMACOLOGY LA English DT Article; Proceedings Paper CT INTERNATIONAL SYMP ON MAST CELL / 7TH ANNUAL SYMP ON THE SKIN PHARMACOLOGY SOC : IMMUNOPHARMACOLOGY AND CARCINOGENESIS CY OCT 30-31, 1990 CL HIROSHIMA, JAPAN SP SKIN PHARMACOL SOC DE INTERLEUKIN-4; FC-RECEPTOR; MAST CELLS; BASOPHILS; LYMPHOKINES ID STIMULATORY FACTOR-I; SPLENIC NON-B; NON-T CELLS; MAST-CELLS; CROSS-LINKAGE; RECEPTOR; IGE; LYMPHOKINES AB Among non-B, non-T cells in the spleen and among unfractionated bone marrow cells, there is a population of cells that are capable of producing IL-4 in response to cross-linkage of Fc-epsilon-RI or Fc-gamma-RII. Their IL-4-producing capacity is strikingly enhanced by treatment of the cells or of the animals donating such cells with interleukin-3 (IL-3). Fc-epsilon-R+ cells constitute 1-2 % of splenic non-B, non-T cells and of bone marrow cells from normal donors but they contain all the capacity to produce IL-4 in response to cross-linkage of Fc-epsilon-RI or Fc-gamma-RII or to treatment with ionomycin. Fc-epsilon-R- cells fall to make such responses. Electron-microscopic analysis indicates that virtually all the granulated or vacuolated Fc-epsilon-R+ cells are of the basophil lineage. However, it has not yet been resolved whether these cells or immature mast cells, presumably in the Fc-epsilon-R+ granule/vacuole-negative cell population, are the principal producers of IL-4 in response to these stimuli. RP PAUL, WE (reprint author), NIAID,IMMUNOL LAB,BLDG 10,RM 11N31,BETHESDA,MD 20892, USA. NR 18 TC 8 Z9 8 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1011-0283 J9 SKIN PHARMACOL JI Skin Pharmacol. PY 1991 VL 4 SU 1 BP 8 EP 14 PG 7 WC Dermatology; Pharmacology & Pharmacy SC Dermatology; Pharmacology & Pharmacy GA GR551 UT WOS:A1991GR55100003 PM 1837220 ER PT J AU HENNINGS, H LOWRY, DT ROBINSON, VA AF HENNINGS, H LOWRY, DT ROBINSON, VA TI COCULTURE OF NEOPLASTIC AND NORMAL KERATINOCYTES AS A MODEL TO STUDY TUMOR PROMOTION SO SKIN PHARMACOLOGY LA English DT Article; Proceedings Paper CT INTERNATIONAL SYMP ON MAST CELL / 7TH ANNUAL SYMP ON THE SKIN PHARMACOLOGY SOC : IMMUNOPHARMACOLOGY AND CARCINOGENESIS CY OCT 30-31, 1990 CL HIROSHIMA, JAPAN SP SKIN PHARMACOL SOC DE KERATINOCYTES; COCULTURE; COMMUNICATION; TUMOR PROMOTION ID MOUSE EPIDERMAL-CELLS; JUNCTIONAL COMMUNICATION; TRANSFORMED PHENOTYPE; CALCIUM REGULATION; SKIN; DIFFERENTIATION; INHIBITION; CULTURE; 12-O-TETRADECANOYLPHORBOL-13-ACETATE; BRYOSTATIN-1 AB In order study skin tumor promotion, a cell culture model system analogous to initiated mouse epidermis was developed. Keratinocytes of the neoplastic cell line 308 display the initiated phenotype since papillomas are produced when the cells are grafted to the backs of athymic mice. Coculture of a small number of these initiated cells with confluent normal keratinocytes results in the suppression of growth of colonies of 308 cells. This inhibition, which is calcium dependent, epidermal cell specific, and requires cell contact, can be overcome by exposure to various tumor promoters. Promoters and antipromoters of diverse structure and mechanism of action are recognized in this keratinocyte coculture model. RP HENNINGS, H (reprint author), NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892, USA. NR 24 TC 8 Z9 8 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1011-0283 J9 SKIN PHARMACOL JI Skin Pharmacol. PY 1991 VL 4 SU 1 BP 79 EP 84 PG 6 WC Dermatology; Pharmacology & Pharmacy SC Dermatology; Pharmacology & Pharmacy GA GR551 UT WOS:A1991GR55100010 PM 1764251 ER PT J AU GOTAY, CC AF GOTAY, CC TI ACCRUAL TO CANCER CLINICAL-TRIALS - DIRECTIONS FROM THE RESEARCH LITERATURE SO SOCIAL SCIENCE & MEDICINE LA English DT Article DE CLINICAL TRIALS; NEOPLASMS; PATIENT PARTICIPATION; RANDOMIZED CONTROLLED TRIALS ID CELL LUNG-CANCER; INFORMED CONSENT; BREAST-CANCER; PHYSICIAN PARTICIPATION; ADJUVANT THERAPY; PATIENT; CHEMOTHERAPY; PREFERENCES; QUALITY; LIFE C1 NCI,DIV CANC PREVENT & CONTROL,BETHESDA,MD 20892. NR 63 TC 123 Z9 123 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0277-9536 J9 SOC SCI MED JI Soc. Sci. Med. PY 1991 VL 33 IS 5 BP 569 EP 577 DI 10.1016/0277-9536(91)90214-W PG 9 WC Public, Environmental & Occupational Health; Social Sciences, Biomedical SC Public, Environmental & Occupational Health; Biomedical Social Sciences GA GG310 UT WOS:A1991GG31000004 PM 1962228 ER PT J AU WILLIAMS, DR LARSON, DB BUCKLER, RE HECKMANN, RC PYLE, CM AF WILLIAMS, DR LARSON, DB BUCKLER, RE HECKMANN, RC PYLE, CM TI RELIGION AND PSYCHOLOGICAL DISTRESS IN A COMMUNITY SAMPLE SO SOCIAL SCIENCE & MEDICINE LA English DT Article DE RELIGION; PSYCHOLOGICAL DISTRESS; STRESS ID MENTAL STATUS; LIFE EVENTS; HEALTH C1 YALE UNIV,DEPT EPIDEMIOL,NEW HAVEN,CT 06520. YALE UNIV,DEPT PUBL HLTH,NEW HAVEN,CT 06520. NIMH,ROCKVILLE,MD 20857. WESTERN SEMINARY,DEPT PSYCHOL,PORTLAND,OR 97215. UNIV COLORADO,SCH MED,DENVER,CO 80262. YALE UNIV,SCH MED,DEPT EPIDEMIOL,NEW HAVEN,CT 06520. YALE UNIV,SCH MED,DEPT PUBL HLTH,NEW HAVEN,CT 06520. RP WILLIAMS, DR (reprint author), YALE UNIV,DEPT SOCIOL,YALE STN,POB 1965,NEW HAVEN,CT 06520, USA. NR 37 TC 137 Z9 138 U1 1 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0277-9536 J9 SOC SCI MED JI Soc. Sci. Med. PY 1991 VL 32 IS 11 BP 1257 EP 1262 DI 10.1016/0277-9536(91)90040-J PG 6 WC Public, Environmental & Occupational Health; Social Sciences, Biomedical SC Public, Environmental & Occupational Health; Biomedical Social Sciences GA FL672 UT WOS:A1991FL67200006 PM 2068608 ER PT J AU BRADBURY, A MILSTEIN, C KOZAK, CA AF BRADBURY, A MILSTEIN, C KOZAK, CA TI CHROMOSOMAL LOCALIZATION OF CD1D GENES IN THE MOUSE SO SOMATIC CELL AND MOLECULAR GENETICS LA English DT Note ID MONOCLONAL-ANTIBODY; ANTIGEN; LINKAGE; HISTOCOMPATIBILITY; SURFACE; FAMILY; MURINE; LINES; CELLS AB Southern blot hybridization of DNA from Chinese hamster X mouse somatic cell hybrids was used to assign the mouse Cd1d genes to chromosome 3. Analysis of the progeny of an intersubspecies backcross was used to position these genes near the gene for glucocerebrosidase, Gba. C1 MRC,MOLEC BIOL LAB,CAMBRIDGE,ENGLAND. NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. RP BRADBURY, A (reprint author), INST NEUROBIOL,15 VIALE CARLO MARX,I-00137 ROME,ITALY. FU NCI NIH HHS [N01-CB2-5584] NR 20 TC 11 Z9 11 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0740-7750 J9 SOMAT CELL MOLEC GEN JI Somat.Cell Mol.Genet. PD JAN PY 1991 VL 17 IS 1 BP 93 EP 96 DI 10.1007/BF01233208 PG 4 WC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity GA FA370 UT WOS:A1991FA37000008 PM 1705364 ER PT J AU YAMADA, M WONDISFORD, FE RADOVICK, S NAKAYAMA, Y WEINTRAUB, BD WILBER, JF AF YAMADA, M WONDISFORD, FE RADOVICK, S NAKAYAMA, Y WEINTRAUB, BD WILBER, JF TI ASSIGNMENT OF HUMAN PREPROTHYROTROPIN-RELEASING HORMONE (TRH) GENE TO CHROMOSOME-3 SO SOMATIC CELL AND MOLECULAR GENETICS LA English DT Note ID PARAVENTRICULAR NUCLEUS; RAT HYPOTHALAMUS; EXPRESSION; LINKAGE; HYBRIDS AB The human gene encoding prepro TRH (thyrotropin-releasing hormone) was assigned to chromosome 3, using human-Chinese hamster ovary somatic cell hybrids, analyzed by Southern hybridizations. Hybridization was carried out with a P-32-labeled human prepro TRH cDNA labeled by the method of random priming (3). Hybridization of the cDNA probe to a human specific 4.8-kb DNA fragment of EcoRI-digested WBC DNA was used to localize the human prepro TRH gene. No hybridization, by contrast, was seen with human prepro TRH cDNA probe and hamster DNA after EcoRI treatment. Results from 29 somatic cell hybrids corroborated unequivocally that the human prepro TRH gene can be assigned to human chromosome 3. C1 NIDDKD,MOLEC CELLULAR & NUTR ENDOCRINOL BRANCH,BETHESDA,MD 20892. RP YAMADA, M (reprint author), UNIV MARYLAND,DEPT MED,DIV ENDOCRINOL,BALTIMORE,MD 21201, USA. NR 13 TC 6 Z9 6 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0740-7750 J9 SOMAT CELL MOLEC GEN JI Somat.Cell Mol.Genet. PD JAN PY 1991 VL 17 IS 1 BP 97 EP 100 DI 10.1007/BF01233209 PG 4 WC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity GA FA370 UT WOS:A1991FA37000009 PM 1900134 ER PT B AU KRAUSE, RM AF KRAUSE, RM BE JELJASZEWICZ, J CIBOROWSKI, P TI THE ELUSIVE HISTORY OF STAPHYLOCOCCAL IMMUNITY - A SEARCH FROM MIRAGE TO REALITY SO STAPHYLOCOCCI SE ZENTRALBLATT FUR BAKTERIOLOGIE : SUPPLEMENT LA English DT Proceedings Paper CT 6TH INTERNATIONAL SYMP ON STAPHYLOCOCCI AND STAPHYLOCOCCAL INFECTIONS CY SEP 04-08, 1989 CL WARSAW, POLAND SP NATL INST HYG POLAND, POLISH MED ASSOC, SECT CHEMOTHERAPY RP KRAUSE, RM (reprint author), NIH,BETHESDA,MD 20892, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU GUSTAV FISCHER VERLAG PI STUTTGART PA STUTTGART BN 3-437-11344-5 J9 ZBL BAKT S PY 1991 VL 21 BP 3 EP 11 PG 9 WC Immunology; Microbiology; Virology SC Immunology; Microbiology; Virology GA BT59K UT WOS:A1991BT59K00001 ER PT B AU HARRIS, CC AF HARRIS, CC BE WAXMAN, S ROSSI, GB TAKAKU, F TI DIFFERENTIATION CONTROL IN NORMAL TRANSFORMED EPITHELIAL-CELLS SO STATUS OF DIFFERENTATION THERAPY OF CANCER, VOL 2 SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT 4TH CONF ON DIFFERENTIATION THERAPY CY NOV 04-09, 1990 CL NAGARA VILLAGE, JAPAN SP ARES SERONO SYMP, SANKYO FDN LIFE SCI, UEHARA MEM FDN, MOCHIDA MEM FDN MED & PHARM RES, KIRIN BREWERY, SANKYO, FUJISAWA PHARM, YAMANOUCHI PHARM, EISAI, SHIONOGI PHARM RP HARRIS, CC (reprint author), NCI,HUMAN CARCINOGENESIS LAB,BLDG 37,ROOM 2CO1,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-792-2 J9 SERONO SYM PY 1991 VL 82 BP 3 EP 4 PG 2 WC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy GA BU53W UT WOS:A1991BU53W00001 ER PT B AU LEVENS, D AF LEVENS, D BE WAXMAN, S ROSSI, GB TAKAKU, F TI STRATEGIES FOR MODIFYING GENE-EXPRESSION DURING DIFFERENTIATION SO STATUS OF DIFFERENTATION THERAPY OF CANCER, VOL 2 SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT 4TH CONF ON DIFFERENTIATION THERAPY CY NOV 04-09, 1990 CL NAGARA VILLAGE, JAPAN SP ARES SERONO SYMP, SANKYO FDN LIFE SCI, UEHARA MEM FDN, MOCHIDA MEM FDN MED & PHARM RES, KIRIN BREWERY, SANKYO, FUJISAWA PHARM, YAMANOUCHI PHARM, EISAI, SHIONOGI PHARM RP LEVENS, D (reprint author), NCI,PATHOL LAB,BLDG 10,ROOM 2N105,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-792-2 J9 SERONO SYM PY 1991 VL 82 BP 47 EP 52 PG 6 WC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy GA BU53W UT WOS:A1991BU53W00007 ER PT B AU BOYD, J BARRETT, JC AF BOYD, J BARRETT, JC BE WAXMAN, S ROSSI, GB TAKAKU, F TI TUMOR SUPPRESSOR GENES, GROWTH-REGULATION AND DIFFERENTIATION SO STATUS OF DIFFERENTATION THERAPY OF CANCER, VOL 2 SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT 4TH CONF ON DIFFERENTIATION THERAPY CY NOV 04-09, 1990 CL NAGARA VILLAGE, JAPAN SP ARES SERONO SYMP, SANKYO FDN LIFE SCI, UEHARA MEM FDN, MOCHIDA MEM FDN MED & PHARM RES, KIRIN BREWERY, SANKYO, FUJISAWA PHARM, YAMANOUCHI PHARM, EISAI, SHIONOGI PHARM RP BOYD, J (reprint author), NIEHS,MOLEC CARCINOGENESIS LAB,GENE EXPRESS SECT,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-792-2 J9 SERONO SYM PY 1991 VL 82 BP 61 EP 67 PG 7 WC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy GA BU53W UT WOS:A1991BU53W00009 ER PT B AU LEHMAN, TA PFEIFER, AMA METCALF, RA BENNETT, WP WESTON, A GERWIN, BI HARRIS, CC AF LEHMAN, TA PFEIFER, AMA METCALF, RA BENNETT, WP WESTON, A GERWIN, BI HARRIS, CC BE WAXMAN, S ROSSI, GB TAKAKU, F TI ONCOGENES AND TUMOR SUPPRESSOR GENES IN HUMAN LUNG CARCINOGENESIS SO STATUS OF DIFFERENTATION THERAPY OF CANCER, VOL 2 SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT 4TH CONF ON DIFFERENTIATION THERAPY CY NOV 04-09, 1990 CL NAGARA VILLAGE, JAPAN SP ARES SERONO SYMP, SANKYO FDN LIFE SCI, UEHARA MEM FDN, MOCHIDA MEM FDN MED & PHARM RES, KIRIN BREWERY, SANKYO, FUJISAWA PHARM, YAMANOUCHI PHARM, EISAI, SHIONOGI PHARM RP LEHMAN, TA (reprint author), NCI,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-792-2 J9 SERONO SYM PY 1991 VL 82 BP 87 EP 98 PG 12 WC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy GA BU53W UT WOS:A1991BU53W00012 ER PT B AU CHOCHUNG, YS YOKOZAKI, H TORTORA, G PEPE, S CLAIR, T AF CHOCHUNG, YS YOKOZAKI, H TORTORA, G PEPE, S CLAIR, T BE WAXMAN, S ROSSI, GB TAKAKU, F TI 8-CL-CAMP IN DIFFERENTIATION THERAPY - THE REVERSAL AND SUPPRESSION OF MALIGNANCY TARGETING THE INTRACELLULAR TRANSDUCING PROTEINS OF CAMP SO STATUS OF DIFFERENTATION THERAPY OF CANCER, VOL 2 SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT 4TH CONF ON DIFFERENTIATION THERAPY CY NOV 04-09, 1990 CL NAGARA VILLAGE, JAPAN SP ARES SERONO SYMP, SANKYO FDN LIFE SCI, UEHARA MEM FDN, MOCHIDA MEM FDN MED & PHARM RES, KIRIN BREWERY, SANKYO, FUJISAWA PHARM, YAMANOUCHI PHARM, EISAI, SHIONOGI PHARM RP CHOCHUNG, YS (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,CELLULAR BIOCHEM SECT,BLDG 10,ROOM 5B38,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-792-2 J9 SERONO SYM PY 1991 VL 82 BP 185 EP 198 PG 14 WC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy GA BU53W UT WOS:A1991BU53W00022 ER PT B AU TAKAHASHI, N BREITMAN, TR AF TAKAHASHI, N BREITMAN, TR BE WAXMAN, S ROSSI, GB TAKAKU, F TI RETINOIC ACID ACYLATION (RETINOYLATION) IN CELL-LINES AND FRESH CELLS FROM A PATIENT WITH ACUTE MYELOGENOUS LEUKEMIA SO STATUS OF DIFFERENTATION THERAPY OF CANCER, VOL 2 SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT 4TH CONF ON DIFFERENTIATION THERAPY CY NOV 04-09, 1990 CL NAGARA VILLAGE, JAPAN SP ARES SERONO SYMP, SANKYO FDN LIFE SCI, UEHARA MEM FDN, MOCHIDA MEM FDN MED & PHARM RES, KIRIN BREWERY, SANKYO, FUJISAWA PHARM, YAMANOUCHI PHARM, EISAI, SHIONOGI PHARM RP TAKAHASHI, N (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,BIOL CHEM LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-792-2 J9 SERONO SYM PY 1991 VL 82 BP 255 EP 264 PG 10 WC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy GA BU53W UT WOS:A1991BU53W00029 ER PT B AU JETTEN, AM NERVI, C SAUNDERS, NA VOLLBERG, TM AF JETTEN, AM NERVI, C SAUNDERS, NA VOLLBERG, TM BE WAXMAN, S ROSSI, GB TAKAKU, F TI REGULATION OF GENE-EXPRESSION BY RETINOIC ACID IN EMBRYONAL CARCINOMA PCC4.AZA1R CELLS SO STATUS OF DIFFERENTATION THERAPY OF CANCER, VOL 2 SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT 4TH CONF ON DIFFERENTIATION THERAPY CY NOV 04-09, 1990 CL NAGARA VILLAGE, JAPAN SP ARES SERONO SYMP, SANKYO FDN LIFE SCI, UEHARA MEM FDN, MOCHIDA MEM FDN MED & PHARM RES, KIRIN BREWERY, SANKYO, FUJISAWA PHARM, YAMANOUCHI PHARM, EISAI, SHIONOGI PHARM RP JETTEN, AM (reprint author), NIEHS,PULM PATHOBIOL LAB,CELL BIOL SECT,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-792-2 J9 SERONO SYM PY 1991 VL 82 BP 265 EP 271 PG 7 WC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy GA BU53W UT WOS:A1991BU53W00030 ER PT B AU CHOCHUNG, YS TORTORA, G PEPE, S YOKOZAKI, H AF CHOCHUNG, YS TORTORA, G PEPE, S YOKOZAKI, H BE WAXMAN, S ROSSI, GB TAKAKU, F TI THE DIFFERENTIATION OF HL-60 HUMAN PROMYELOCYTIC LEUKEMIA BY 8-CL-CAMP AND RHGM-CSF IN COMBINATION SO STATUS OF DIFFERENTATION THERAPY OF CANCER, VOL 2 SE SERONO SYMPOSIA PUBLICATIONS FROM RAVEN PRESS LA English DT Proceedings Paper CT 4TH CONF ON DIFFERENTIATION THERAPY CY NOV 04-09, 1990 CL NAGARA VILLAGE, JAPAN SP ARES SERONO SYMP, SANKYO FDN LIFE SCI, UEHARA MEM FDN, MOCHIDA MEM FDN MED & PHARM RES, KIRIN BREWERY, SANKYO, FUJISAWA PHARM, YAMANOUCHI PHARM, EISAI, SHIONOGI PHARM RP CHOCHUNG, YS (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,CELLULAR BIOCHEM SECT,BLDG 10,ROOM 5B38,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-792-2 J9 SERONO SYM PY 1991 VL 82 BP 393 EP 398 PG 6 WC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy GA BU53W UT WOS:A1991BU53W00044 ER PT B AU PUTNEY, JW BIRD, GSJ HORSTMAN, DA HUGHES, AR MENNITI, FS NOGIMORI, K OBIE, J OLIVER, KG SUGIYA, H TAKEMURA, H AF PUTNEY, JW BIRD, GSJ HORSTMAN, DA HUGHES, AR MENNITI, FS NOGIMORI, K OBIE, J OLIVER, KG SUGIYA, H TAKEMURA, H BE LEEMAN, SE KRAUSE, JE LEMBECK, F TI ROLE OF INOSITOL PHOSPHATES IN THE ACTIONS OF SUBSTANCE-P ON NK1 RECEPTORS IN EXOCRINE GLAND-CELLS SO SUBSTANCE P AND RELATED PEPTIDES : CELLULAR AND MOLECULAR PHYSIOLOGY SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON SUBSTANCE P AND RELATED PEPTIDES : CELLULAR AND MOLECULAR PHYSIOLOGY CY JUL 18-21, 1990 CL WORCESTER, MA SP NEW YORK ACAD SCI, UNIV MASSACHUETTS, MED CTR, EASTMAN KODAK, NIH NINDS, NATL SCI FDN, USA MED RES & DEV COMMAND, BRISTOL MYERS, CIBA GEIGY, DUPONT CO, ELI LILLY CO RP PUTNEY, JW (reprint author), NIEHS,CELLULAR & MOLEC PHARMACOL,CALCIUM REGULAT SECT,RES TRIANGLE PK,NC 27709, USA. OI Menniti, Frank/0000-0003-2612-9534 NR 0 TC 14 Z9 14 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-664-X J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 632 BP 94 EP 102 DI 10.1111/j.1749-6632.1991.tb33097.x PG 9 WC Biochemistry & Molecular Biology; Physiology SC Biochemistry & Molecular Biology; Physiology GA BW41B UT WOS:A1991BW41B00010 PM 1719914 ER PT J AU SILKS, LA ODOM, JD DUNLAP, RB AF SILKS, LA ODOM, JD DUNLAP, RB TI SYNTHESIS AND TE-125 NMR-SPECTROSCOPY OF ALPHA-TELLUROCARBONYL COMPOUNDS AND DERIVATIVES SO SYNTHETIC COMMUNICATIONS LA English DT Article ID SODIUM HYDROGEN TELLURIDE; ORGANOTELLURIUM CHEMISTRY; TELLUROLATE REAGENT; REDUCTIVE REMOVAL; KETONES; SUBSTITUENTS; ACIDS AB The reaction of lithium alkyl-, alkenyl-, alkynyl-, and aryl tellurolates with alpha-bromocarbonyl compounds in anhydrous tetrahydrofuran gives the title compounds in yields ranging from 55-92%. The Te-125 NMR chemical shift range for these compounds is 405-1024 ppm. C1 UNIV S CAROLINA,DEPT CHEM,COLUMBIA,SC 29208. RP SILKS, LA (reprint author), LOS ALAMOS NATL LAB,NIH,NATL STABLE ISOTOPES RESOURCE,INC-4,MS C-345,LOS ALAMOS,NM 87545, USA. NR 24 TC 13 Z9 13 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0039-7911 J9 SYNTHETIC COMMUN JI Synth. Commun. PY 1991 VL 21 IS 8-9 BP 1105 EP 1119 DI 10.1080/00397919108019802 PG 15 WC Chemistry, Organic SC Chemistry GA FT878 UT WOS:A1991FT87800020 ER PT B AU MARTIN, BM ELIASON, WK AF MARTIN, BM ELIASON, WK BE VILLAFRANCA, JJ TI GLYCOPEPTIDASE-F TREATMENT AND AMINO-ACID-SEQUENCE DETERMINATION OF GLYCOPROTEINS IMMOBILIZED ON PVDF AND ITS DERIVATIVES SO TECHNIQUES IN PROTEIN CHEMISTRY II LA English DT Proceedings Paper CT 4TH ANNUAL SYMP OF THE PROTEIN SOC CY AUG 11-15, 1990 CL SAN DIEGO, CA SP PROTEIN SOC, A A A LAB, APPL BIOSYST, A V I V ASSOC, BECKMAN INSTRUMENTS, BIOSYM TECHNOL, BRISTOL MYERS SQUIBB PHARM RES INST, DUPONT CO, ELI LILLY & CO, FINNIGAN RP MARTIN, BM (reprint author), NIMH,CLIN NEUROSCI BRANCH,MOLEC NEUROGENET SECT,BETHESDA,MD 20892, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA SAN DIEGO BN 0-12-721957-9 PY 1991 BP 191 EP 196 PG 6 WC Biochemistry & Molecular Biology; Medical Laboratory Technology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Medical Laboratory Technology; Research & Experimental Medicine GA BT33C UT WOS:A1991BT33C00018 ER PT B AU BALDWIN, ET SUSSMAN, J STCHARLES, R WLODAWER, A AF BALDWIN, ET SUSSMAN, J STCHARLES, R WLODAWER, A BE VILLAFRANCA, JJ TI MOLECULAR REPLACEMENT STRUCTURE SOLUTION OF INTERLEUKIN-8 USING THE NMR MODEL SO TECHNIQUES IN PROTEIN CHEMISTRY II LA English DT Proceedings Paper CT 4TH ANNUAL SYMP OF THE PROTEIN SOC CY AUG 11-15, 1990 CL SAN DIEGO, CA SP PROTEIN SOC, A A A LAB, APPL BIOSYST, A V I V ASSOC, BECKMAN INSTRUMENTS, BIOSYM TECHNOL, BRISTOL MYERS SQUIBB PHARM RES INST, DUPONT CO, ELI LILLY & CO, FINNIGAN RP BALDWIN, ET (reprint author), NCI,FREDERICK CANC RES & DEV CTR,CRYSTALLOG LAB,ABL BASIC RES PROGRAM,FREDERICK,MD 21701, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA SAN DIEGO BN 0-12-721957-9 PY 1991 BP 347 EP 356 PG 10 WC Biochemistry & Molecular Biology; Medical Laboratory Technology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Medical Laboratory Technology; Research & Experimental Medicine GA BT33C UT WOS:A1991BT33C00033 ER PT J AU MIRKES, PE LITTLE, SA BELAND, FA HUITFELDT, HS POIRIER, MC AF MIRKES, PE LITTLE, SA BELAND, FA HUITFELDT, HS POIRIER, MC TI QUANTITATION AND IMMUNOHISTOCHEMICAL LOCALIZATION OF DNA ADDUCTS IN RAT EMBRYOS AND ASSOCIATED YOLK-SAC MEMBRANES EXPOSED INVITRO TO N-ACETOXY-2-ACETYLAMINOFLUORENE (N-AC-AAF) SO TERATOGENESIS CARCINOGENESIS AND MUTAGENESIS LA English DT Article DE C-8; SUBSTITUTED DEOXYGUANOSINE ADDUCTS; DEACETYLATED DNA ADDUCTS; N-AC-AAF; RAT EMBRYO ID TERATOGENICITY INVITRO; LIVER; 2-ACETYLAMINOFLUORENE; REMOVAL; CARCINOGENESIS; BIOTRANSFORMATION; RADIOIMMUNOASSAY; METABOLITES; MECHANISM; BINDING AB Specific antibodies and radioimmunoassay (RIA) were used to measure the levels of acetylated and deacetylated C-8 substituted deoxyguanosine adducts in day 11 rat embryos and their associated yolk sacs after exposure of whole rat conceptuses in vitro to the teratogen N-acetoxy-2-acetylaminofluorene (N-Ac-AAF). The deacetylated adduct predominates in both the embryo and the associated yolk sac, and a dose response for adduct formation was observed when adducts were quantitated by RIA. Immunohistochemical localization of the deacetylated adducts revealed that adducts were confined to the nuclei in all tissues examined and that the abundance of adducts varied within and between tissues. Our initial findings indicate that specific DNA adduct antibodies may be useful in the study of teratogenesis induced by a wide variety of agents that modify DNA. C1 UNIV WASHINGTON,CTR CHILD DEV & MENTAL RETARDAT,SEATTLE,WA 98195. NATL CTR TOXICOL RES,JEFFERSON,AR 72079. NATL INST PUBL HLTH,DEPT ENVIRONM MED,OSLO 1,NORWAY. NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892. RP MIRKES, PE (reprint author), UNIV WASHINGTON,DEPT PEDIAT,SEATTLE,WA 98195, USA. FU NICHD NIH HHS [HD 22095, HD 16287] NR 29 TC 6 Z9 6 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0270-3211 J9 TERATOGEN CARCIN MUT JI Teratogenesis Carcinog. Mutagen. PY 1991 VL 11 IS 2 BP 93 EP 102 DI 10.1002/tcm.1770110205 PG 10 WC Oncology; Genetics & Heredity; Toxicology SC Oncology; Genetics & Heredity; Toxicology GA GG503 UT WOS:A1991GG50300004 PM 1686678 ER PT J AU PIEGORSCH, WW HASEMAN, JK AF PIEGORSCH, WW HASEMAN, JK TI STATISTICAL-METHODS FOR ANALYZING DEVELOPMENTAL TOXICITY DATA SO TERATOGENESIS CARCINOGENESIS AND MUTAGENESIS LA English DT Review DE BETA-BINOMIAL MODEL; DOMINANT LETHAL TEST; GENERALIZED BINOMIAL MODELS; GENOTOXICITY; INTRALITTER CORRELATION; OVERDISPERSION; QUASILIKELIHOOD ESTIMATION; STATISTICAL RESAMPLING PLANS ID BETA-BINOMIAL DISTRIBUTION; QUANTITATIVE RISK ANALYSIS; LONGITUDINAL DATA-ANALYSIS; DOMINANT LETHAL MUTATIONS; GENERALIZED LINEAR-MODELS; DICHOTOMOUS RESPONSE DATA; WEAK MUTAGENIC ACTIVITY; DOSE-RESPONSE; TOXICOLOGICAL EXPERIMENTS; TERATOLOGICAL EXPERIMENTS AB A description and review of methods for performing per-litter analyses involving extra-binomial proportion response is provided. It is stressed that the litter should be regarded as the appropriate experimental unit for quantitative analysis in studies for teratogenic or heritable mutagenic effects. Attention is directed at statistical identification of possible treatment effects, such as a positive dose response to a chemical stimulus. The methods range from distribution-free, nonparametric analyses to models involving parametric distributions such as the beta-binomial density. It is seen that most current methods require computer implementation. When concern is raised over misspecification of assumptions critical to the statistical analysis, it is argued that relatively parameter-free methods are appropriate for use. These include statistical bootstrapping and rank-based analyses. RP PIEGORSCH, WW (reprint author), NIEHS,STAT & BIOMATH BRANCH,RES TRIANGLE PK,NC 27709, USA. OI Piegorsch, Walter/0000-0003-2725-5604 NR 100 TC 20 Z9 20 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0270-3211 J9 TERATOGEN CARCIN MUT JI Teratogenesis Carcinog. Mutagen. PY 1991 VL 11 IS 3 BP 115 EP 133 DI 10.1002/tcm.1770110302 PG 19 WC Oncology; Genetics & Heredity; Toxicology SC Oncology; Genetics & Heredity; Toxicology GA GK090 UT WOS:A1991GK09000001 PM 1686820 ER PT J AU NEWBOLD, RR JELLINCK, PH METZLER, M MCLACHLAN, JA AF NEWBOLD, RR JELLINCK, PH METZLER, M MCLACHLAN, JA TI ONTOGENY OF PEROXIDASE-ACTIVITY IN EPITHELIUM AND EOSINOPHILS OF THE MOUSE UTERUS SO TERATOGENESIS CARCINOGENESIS AND MUTAGENESIS LA English DT Article DE UTERINE PEROXIDASE; NEONATAL DES; DIETHYLSTILBESTROL; HORMONAL CARCINOGENESIS; UTERINE ADENOCARCINOMA ID ESTROGEN STIMULATION; UTERINE PEROXIDASE; PRENATAL EXPOSURE; GENITAL-TRACT; TUMOR-CELLS; DIETHYLSTILBESTROL; METABOLISM; ESTRADIOL; BINDING; MICE AB Outbred CD- 1 mice treated for 1 or 4 days with 1 mg/kg of diethylstilbestrol (DES) at various ages after birth were examined for histochemical localization of peroxidase in the uterine epithelium. Peroxidase activity in uterine extracts was also measured by a radio-metric assay and the conversion of [H-3]DES to [H-3]Z,Z-dienestrol (Z,Z-DIES). While no peroxidase activity was detected by a histochemical method in uterine epithelium from untreated 5-day old mice, the enzyme was apparent in mice treated for 4 days with DES; uterine eosinophils were absent at this age. By day 9, DES-induced staining for peroxidase in uterine epithelial cells and the number of uterine eosinophils had increased significantly. In addition, at this age. the biochemical assays for uterine peroxidase were sensitive enough to show that DES is converted to Z,Z-DIES and that [H-3]estradiol gives rise to (H2O)-H-3 and water-soluble radioactive metabolites. The peroxidase response to DES, determined by both histochemical and biochemical methods, increased with the age of the immature mice. These data indicate that the neonatal uterus, although deficient in eosinophils, demonstrates a peroxidase response to estrogen and that this response is localized primarily in the luminal epithelium. The role of this DES-induced peroxidase activity in converting DES to activated metabolites that may cause cell damage is discussed. C1 QUEENS UNIV,DEPT BIOCHEM,KINGSTON K7L 3N6,ONTARIO,CANADA. UNIV KAISERSLAUTERN,DEPT FOOD CHEM & ENVIRONM TOXICOL,W-6750 KAISERSLAUTERN,GERMANY. RP NEWBOLD, RR (reprint author), NIEHS,REPROD & DEV TOXICOL,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 50 TC 3 Z9 3 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0270-3211 J9 TERATOGEN CARCIN MUT JI Teratogenesis Carcinog. Mutagen. PY 1991 VL 11 IS 5 BP 267 EP 278 DI 10.1002/tcm.1770110507 PG 12 WC Oncology; Genetics & Heredity; Toxicology SC Oncology; Genetics & Heredity; Toxicology GA HR614 UT WOS:A1991HR61400006 PM 1687904 ER PT J AU WATANABE, T PRATT, RM AF WATANABE, T PRATT, RM TI INFLUENCE OF RETINOIDS ON SISTER CHROMATID EXCHANGES AND CHROMOSOMES IN CULTURED HUMAN EMBRYONIC PALATAL MESENCHYMAL CELLS SO TERATOGENESIS CARCINOGENESIS AND MUTAGENESIS LA English DT Article DE RETINOIC ACIDS; METABOLIC ACTIVATION; CHROMOSOME ABERRATIONS; HEPM CELLS; SCE ID SCREENING ASSAY; V79 CELLS; VITAMIN-A; GROWTH; PROLIFERATION; INHIBITION; INDUCTION; ACID AB The cytogenetic effects of retinoids and their metabolites on human embryonic palatal mesenchymal (HEPM) cells were investigated in cell culture. Treatment with 13-cis, 4-oxo-13-cis-, and all-trans-retinoic acids did not induce chromosomal structural aberrations even at high concentrations in the presence or absence of metabolic activation system. The frequencies of sister chromatid exchanges (SCE) tended to decrease in HEPM cells treated with retinoids in the absence of S-9. 4-Oxo-13-cis-retinoic acid, which is one metabolite of 13-cis-retinoic acid, significantly decreased the SCE frequencies. The mitotic index decreased with increasing concentration of retinoic acids. indicating that retinoids inhibit cell proliferation in HEPM cells. We concluded that retinoids inhibit cell proliferation in HEPM cells in culture without causing DNA or chromosome damage. C1 NIEHS,REPROD & DEV TOXICOL LAB,RES TRIANGLE PK,NC 27709. RP WATANABE, T (reprint author), YAMAGATA UNIV,SCH MED,DEPT HYG & PREVENT MED,YAMAGATA 99023,JAPAN. NR 20 TC 5 Z9 6 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0270-3211 J9 TERATOGEN CARCIN MUT JI Teratogenesis Carcinog. Mutagen. PY 1991 VL 11 IS 6 BP 297 EP 304 DI 10.1002/tcm.1770110603 PG 8 WC Oncology; Genetics & Heredity; Toxicology SC Oncology; Genetics & Heredity; Toxicology GA HU090 UT WOS:A1991HU09000002 PM 1687838 ER PT B AU DEMENT, JM RINGEN, K SELIKOFF, IJ EGILMAN, D GIBBS, G KILBURN, K CASE, B RICHTER, E LILIS, R AF DEMENT, JM RINGEN, K SELIKOFF, IJ EGILMAN, D GIBBS, G KILBURN, K CASE, B RICHTER, E LILIS, R BE LANDRIGAN, PJ KAZEMI, H TI CARCINOGENICITY OF CHRYSOTILE ASBESTOS - EVIDENCE FROM COHORT STUDIES SO THIRD WAVE OF ASBESTOS DISEASE : EXPOSURE TO ASBESTOS IN PLACE: PUBLIC HEALTH CONTROL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON THE THIRD WAVE OF ASBESTOS DISEASE - EXPOSURE TO PLACE : PUBLIC HEALTH CONTROL CY JUN 07-09, 1990 CL NEW YORK, NY RP DEMENT, JM (reprint author), NIEHS,11 ALEXANDER DR,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 13 Z9 13 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-678-X J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 643 BP 15 EP 26 DI 10.1111/j.1749-6632.1991.tb24440.x PG 12 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA BW42W UT WOS:A1991BW42W00002 PM 1809127 ER PT B AU LASKY, JA BONNER, JC BRODY, AR AF LASKY, JA BONNER, JC BRODY, AR BE LANDRIGAN, PJ KAZEMI, H TI THE PATHOBIOLOGY OF ASBESTOS-INDUCED LUNG-DISEASE - A PROPOSED ROLE FOR MACROPHAGE-DERIVED GROWTH-FACTORS SO THIRD WAVE OF ASBESTOS DISEASE : EXPOSURE TO ASBESTOS IN PLACE: PUBLIC HEALTH CONTROL SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Proceedings Paper CT CONF ON THE THIRD WAVE OF ASBESTOS DISEASE - EXPOSURE TO PLACE : PUBLIC HEALTH CONTROL CY JUN 07-09, 1990 CL NEW YORK, NY RP LASKY, JA (reprint author), NIEHS,PULM PATHOBIOL LAB,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 3 Z9 3 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA NEW YORK BN 0-89766-678-X J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1991 VL 643 BP 239 EP 244 DI 10.1111/j.1749-6632.1991.tb24468.x PG 6 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA BW42W UT WOS:A1991BW42W00023 PM 1809136 ER PT J AU SASS, B BANFIELD, WG SAFFIOTTI, U AF SASS, B BANFIELD, WG SAFFIOTTI, U TI DETECTION AND CELLULAR-LOCALIZATION OF LEAD BY ELECTRON-PROBE ANALYSIS IN THE DIAGNOSIS OF SUSPECTED LEAD-POISONING IN RHESUS-MONKEYS SO TOXICOLOGIC PATHOLOGY LA English DT Article DE KIDNEY; INTRANUCLEAR INCLUSION BODIES; SCANNING ELECTRON MICROSCOPE (SEM); ENERGY DISPERSIVE X-RAY ANALYSIS AB Lead poisoning of unknown source was diagnosed histologically in 2 rhesus monkeys (Macaca mulatta) by finding acid-fast intranuclear inclusion bodies in the epithelial cells of renal cortical tubules. The presence of lead in the inclusions was determined by scanning electron microscopy/energy dispersive x-ray analysis using sections from paraffin embedded tissues. This observation indicates the usefulness of this technique for the detection and cellular localization of lead in tissues, even from archival material. C1 NCI,REGISTRY EXPTL CANC,DIV CANC ETIOL,BLDG 41,ROOM C-105,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC TOXICOLOGIC PATHOLOGISTS PI LAWRENCE PA 1041 NEW HAMPSHIRE ST PO BOX 368, LAWRENCE, KS 66044 SN 0192-6233 J9 TOXICOL PATHOL JI Toxicol. Pathol. PY 1991 VL 19 IS 1 BP 30 EP 34 PG 5 WC Pathology; Toxicology SC Pathology; Toxicology GA FG058 UT WOS:A1991FG05800004 PM 1646479 ER PT J AU REHM, S WARD, JM ANDERSON, LM RIGGS, CW RICE, JM AF REHM, S WARD, JM ANDERSON, LM RIGGS, CW RICE, JM TI TRANSPLACENTAL INDUCTION OF MOUSE LUNG-TUMORS - STAGE OF FETAL ORGANOGENESIS IN RELATION TO FREQUENCY, MORPHOLOGY, SIZE, AND NEOPLASTIC PROGRESSION OF N-NITROSOETHYLUREA-INDUCED TUMORS SO TOXICOLOGIC PATHOLOGY LA English DT Article DE MOUSE; LUNG TUMORS; TRANSPLACENTAL CARCINOGENESIS AB Pregnant C3H/HeNCrMTV- mice were given a single intraperitoneal injection of 0.5 mmol N-nitrosoethylurea/kg on days 14, 16, or 18 of gestation. Six of the male offspring were sacrificed for study at the ages of 2, 4, 8, 16, 32, and 52 weeks. Grossly visible lung tumors were counted and all lungs were sectioned completely, saving every tenth section for histologic evaluation. All N-nitrosoethylurea-induced mouse lung tumors have previously been shown to originate from alveolar type II cells. Lung tumors were diagnosed as solid, papillary, or mixed solid/papillary types, and at the largest area of each tumor, the perimeter was measured and compared with the number of sections per tumor. The fraction of tumors detected grossly depended on size and, on average, only 51% of neoplasms present were detected macroscopically. A significant correlation was seen between the mean number of histological sections and perimeter length per tumor, in particular for small and medium sized papillary neoplasms. The growth of solid tumors was limited to a maximum size, after which they progressed towards papillary types. The numbers of transplacentally induced mouse lung tumors were distributed in direct proportion to the weight of the individual lung lobes, unrelated to day of treatment of type or tumor. Tumor biology depended on the day of treatment reflecting numbers and degree of differentiation of fetal alveolar type II cells, i.e., the target cell: most tumors developed in offspring treated on day 16, tumor size was greater and progression from solid to papillary neoplasms faster at earlier treatments, increase in tumor multiplicity postnatally was only seen in mice treated late in gestation, and mice treated on day 14 or day 16 showed a consistent ratio of solid to papillary tumors. RP REHM, S (reprint author), NCI,FREDERICK CANC RES & DEV CTR,BLDG 538,ROOM 220,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74102] NR 0 TC 13 Z9 13 U1 0 U2 0 PU SOC TOXICOLOGIC PATHOLOGISTS PI LAWRENCE PA 1041 NEW HAMPSHIRE ST PO BOX 368, LAWRENCE, KS 66044 SN 0192-6233 J9 TOXICOL PATHOL JI Toxicol. Pathol. PY 1991 VL 19 IS 1 BP 35 EP 46 PG 12 WC Pathology; Toxicology SC Pathology; Toxicology GA FG058 UT WOS:A1991FG05800005 PM 2047706 ER PT J AU RAO, GN AF RAO, GN TI LIGHT INTENSITY-ASSOCIATED EYE LESIONS OF FISCHER-344 RATS IN LONG-TERM STUDIES SO TOXICOLOGIC PATHOLOGY LA English DT Article DE CATARACTS; RETINAL DEGENERATION; OPACITY; CAGE LOCATION; ALBINO RATS; PREVENTION AB Albino rats and mice are sensitive to light and the recommended illumination of animal rooms (75-125 ft-candles) is known to cause retinal damage. When a room is illuminated by ceiling lights, animals in the cages of the top row and, to some extent, in the side columns of cage racks will be exposed to higher light intensity than those in the other cages of the rack. In 2-yr chemical carcinogenicity studies of the National Toxicology Program (previously the Carcinogenicity Bioassay Program of the National Cancer Institute), Fischer 344 rats were group-housed in hanging drawer-type clear polycarbonate cages. During the course of the chronic studies, a number of rats developed opacity of the eye. Ocular examination indicated chronic uveitis, deep interstitial keratitis, cataract formation leading to panophthalmitis, and in severe cases, phthisis bulbi. Histologic examination showed cataract and retinal degeneration. Incidences of these lesions were highest (> 55%) in the rats of the top rows and lowest in those of the bottom rows (< 10%) of cages with no relation to chemical treatment, indicating an association with light intensity. The incidence of these eye lesions was markedly decreased (< 15%) by decreasing the light intensity of the animal room to < 50 ft-candles at 5 ft from the floor and rotating the cages in each column of a rack from top to bottom when cages or racks were changed. RP RAO, GN (reprint author), NIEHS,NATL TOXICOL PROGRAM,DIV TOXICOL RES & TESTING,MD AO-01,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 24 Z9 24 U1 0 U2 3 PU SOC TOXICOLOGIC PATHOLOGISTS PI LAWRENCE PA 1041 NEW HAMPSHIRE ST PO BOX 368, LAWRENCE, KS 66044 SN 0192-6233 J9 TOXICOL PATHOL JI Toxicol. Pathol. PY 1991 VL 19 IS 2 BP 148 EP 155 PG 8 WC Pathology; Toxicology SC Pathology; Toxicology GA FZ146 UT WOS:A1991FZ14600009 PM 1663269 ER PT J AU GHANAYEM, BI MATTHEWS, HB MARONPOT, RR AF GHANAYEM, BI MATTHEWS, HB MARONPOT, RR TI SUSTAINABILITY OF FORESTOMACH HYPERPLASIA IN RATS TREATED WITH ETHYL ACRYLATE FOR 13 WEEKS AND REGRESSION AFTER CESSATION OF DOSING SO TOXICOLOGIC PATHOLOGY LA English DT Article DE TOXICOLOGY, F344 MALE RATS; ETHYL ACRYLATE, FORESTOMACH TOXICITY; FORESTOMACH HYPERPLASIA, SUSTAINABILITY; FORESTOMACH HYPERPLASIA, REVERSIBILITY; FORESTOMACH CELL PROLIFERATION, CARCINOGENESIS AB In a chronic study conducted by the National Toxicology Program (NTP), gavage administration of 100 or 200 mg ethyl acrylate (EA)/kg/day, 5 days/week, to F344 rats and B6C3F1 mice resulted in a significant dose-dependent increase in the incidence of squamous cell papillomas and carcinomas of the forestomach of both sexes of rats and mice. No increase in the incidence of tumors was observed at any other site in these rats. Chemically-induced cell proliferation is currently thought to play a role in the development and progression of chemically-induced neoplasia. Therefore, a stop-study was initiated where 100 or 200 mg EA/kg (in com oil) was administered daily, 5 days/week, for 13 weeks. Rats sacrificed at the end of the treatment regimen had severe epithelial hyperplasia of the forestomach. No lesions were observed in the glandular stomach or liver of EA-treated rats. Forestomach hyperplasia induced by EA included upward and downward cell proliferation. However, forestomachs of rats treated for 13 weeks and sacrificed 8 weeks after the last EA dose exhibited a significant decline in the incidence and severity of forestomach mucosal hyperplasia. Histopathologic evaluation of forestomachs of EA-treated rats (13 weeks) which were allowed a 19-month-recovery (with no exposure to EA) showed further decline in the incidence and severity of mucosal cell hyperplasia. These results indicate that gavage administration of EA to rats results in extensive and sustained forestomach mucosal hyperplasia. The sustainability of forestomach hyperplasia is apparently dependent on the continued exposure of rats to ethyl acrylate, and regressed after cessation of dosing. Furthermore, although enough post-treatment time was allowed for tumors to develop after cessation of EA administration, forestomachs exhibited a nearly complete recovery with no increased incidence of papillomas or carcinomas. It, therefore, remains to be determined what duration of exposure or other factors are critical for reversibility or progression of EA-induced forestomach mucosal hyperplasia to neoplasia. RP GHANAYEM, BI (reprint author), NIEHS,NATL TOXICOL PROGRAM,EXPTL TOXICOL BRANCH,RES TRIANGLE PK,NC 27711, USA. NR 0 TC 11 Z9 11 U1 0 U2 0 PU SOC TOXICOLOGIC PATHOLOGISTS PI LAWRENCE PA 1041 NEW HAMPSHIRE ST PO BOX 368, LAWRENCE, KS 66044 SN 0192-6233 J9 TOXICOL PATHOL JI Toxicol. Pathol. PY 1991 VL 19 IS 3 BP 273 EP 279 PG 7 WC Pathology; Toxicology SC Pathology; Toxicology GA GP999 UT WOS:A1991GP99900010 PM 1723532 ER PT J AU HOOK, GER AF HOOK, GER TI ALVEOLAR PROTEINOSIS AND PHOSPHOLIPIDOSES OF THE LUNGS SO TOXICOLOGIC PATHOLOGY LA English DT Article; Proceedings Paper CT 10TH INTERNATIONAL SYMP OF THE SOC OF TOXICOLOGIC PATHOLOGISTS : PULMONARY TOXICOLOGIC PATHOLOGY CY JUN 02-06, 1991 CL MONTEREY, CA SP SOC TOXICOL PATHOLOGISTS DE PHOSPHOLIPID; TYPE-II CELL; TUBULAR MYELIN; MACROPHAGES; SILICA; SURFACTANT; SURFACTANT PROTEINS AB Three pulmonary disease conditions result from the accumulation of phospholipids in the lung. These conditions are the human lung disease known as pulmonary alveolar proteinosis, the lipoproteinosis that arises in the lungs of rats during acute silicosis, and the phospholipidoses induced by numerous cationic amphiphilic therapeutic agents. In this paper, the status of phospholipid metabolism in the lungs during the process of each of these lung conditions has been reviewed and possible mechanisms for their establishment are discussed. Pulmonary alveolar proteinosis is characterized by the accumulation of tubular myelin-like multilamellated structures in the alveoli and distal airways of patients. These structures appear to be formed by a process of spontaneous assembly involving surfactant protein A and surfactant phospholipids. Structures similar to tubular myelin-like multilamellated structures can be seen in the alveoli of rats during acute silicosis and, as with the human condition, both surfactant protein A and surfactant phospholipids accumulate in the alveoli. Excessive accumulation of surfactant protein A and surfactant phospholipids in the alveoli could arise from their overproduction and hypersecretion by a subpopulation of Type II cells that are activated by silica, and possibly other agents. Phospholipidoses caused by cationic amphiphilic therapeutic agents arise as a result of their inhibition of phospholipid catabolism. Inhibition of phospholipases results in the accumulation of phospholipids in the cytoplasm of alveolar macrophages and other cells. While inhibition of phospholipases by these agents undoubtedly occurs, there are many anomalous features, such as the accumulation of extracellular phospholipids and surfactant protein A, that cannot be accounted for by this simplistic hypothesis. RP HOOK, GER (reprint author), NIEHS,PULM PATHOBIOL LAB,BIOCHEM PATHOL GRP,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 43 Z9 43 U1 0 U2 1 PU SOC TOXICOLOGIC PATHOLOGISTS PI LAWRENCE PA 1041 NEW HAMPSHIRE ST PO BOX 368, LAWRENCE, KS 66044 SN 0192-6233 J9 TOXICOL PATHOL JI Toxicol. Pathol. PY 1991 VL 19 IS 4 BP 482 EP 513 PN 1 PG 32 WC Pathology; Toxicology SC Pathology; Toxicology GA HQ749 UT WOS:A1991HQ74900016 PM 1667556 ER PT J AU DIXON, D MARONPOT, RR AF DIXON, D MARONPOT, RR TI HISTOMORPHOLOGIC FEATURES OF SPONTANEOUS AND CHEMICALLY-INDUCED PULMONARY NEOPLASMS IN B6C3F1 MICE AND FISCHER 344 RATS SO TOXICOLOGIC PATHOLOGY LA English DT Article; Proceedings Paper CT 10TH INTERNATIONAL SYMP OF THE SOC OF TOXICOLOGIC PATHOLOGISTS : PULMONARY TOXICOLOGIC PATHOLOGY CY JUN 02-06, 1991 CL MONTEREY, CA SP SOC TOXICOL PATHOLOGISTS DE HISTOMORPHOLOGY; ULTRASTRUCTURAL; LUNG TUMORS; CLARA CELLS; ALVEOLAR TYPE-II CELL AB The histomorphologic features of spontaneous and chemically-induced lung neoplasms in male and female B6C3F1 mice and Fischer 344 rats are described. Primary pulmonary neoplasms in mice and rats were classified as alveolar/bronchiolar (A/B) adenoma or carcinoma (including variants with squamous and mucinous cell differentiation), bronchial adenoma or carcinoma, squamous cell carcinoma or mesenchymal tumors. A/B adenomas and carcinomas were the most common spontaneous pulmonary neoplasms observed in both mice and rats, but were observed less frequently in rats. In the National Toxicology Program (NTP) historical control database the incidence of spontaneous A/B adenomas in male (n = 2,084) and female (n = 2,079) mice is 13.8% and 4.9%, respectively; for A/B carcinomas, it is 5.3% and 2.4%, respectively. In male (n = 3,877) and female (n = 3,919) rats, spontaneous pulmonary neoplasms are rare with historical control rates less than 3% for A/B adenomas or carcinomas in either sex. The spontaneous A/B adenomas and carcinomas observed in mice and rats typically had papillary, solid or mixed (papillary and solid) histologic growth patterns. Pulmonary neoplasms from mice and rats treated with chemical carcinogens reviewed from 2-year studies consisted primarily of A/B adenomas and carcinomas. These tumors had papillary, glandular/tubular, solid or mixed (combination of 2 or more) histologic growth patterns. A few of the A/B neoplasms had areas of squamous or mucinous cell differentiation. Other less frequently occurring spontaneous and chemically-induced neoplasms included squamous cell carcinomas, bronchial adenomas and carcinomas, and sarcomas. RP DIXON, D (reprint author), NIEHS,NATL TOXICOL PROGRAM,POB 12233,MDC2-09,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 7 Z9 7 U1 0 U2 0 PU SOC TOXICOLOGIC PATHOLOGISTS PI LAWRENCE PA 1041 NEW HAMPSHIRE ST PO BOX 368, LAWRENCE, KS 66044 SN 0192-6233 J9 TOXICOL PATHOL JI Toxicol. Pathol. PY 1991 VL 19 IS 4 BP 540 EP 556 PN 1 PG 17 WC Pathology; Toxicology SC Pathology; Toxicology GA HQ749 UT WOS:A1991HQ74900019 PM 1813992 ER PT J AU MARONPOT, RR GILES, HD DYKES, DJ IRWIN, RD AF MARONPOT, RR GILES, HD DYKES, DJ IRWIN, RD TI FURAN-INDUCED HEPATIC CHOLANGIOCARCINOMAS IN FISCHER-344 RATS SO TOXICOLOGIC PATHOLOGY LA English DT Article DE CHOLANGIOHEPATITIS; HEPATOCARCINOGENESIS; TRANSPLANTATION; TUMOR PROGRESSION; STOP STUDY AB In a 2-yr carcinogenicity bioassay, 0, 2, 4, or 8 mg furan/kg body weight (BW) was administered to male and female Fischer (F344) rats and resulted in an 86-100% incidence of cholangiocarcinomas with occasional metastasis. In a separate but concurrent study, male F344 rats dosed with 30 mg furan/kg BW for 90 days developed marked cholangiofibrosis and cholangiohepatitis and, when subsequently maintained without further treatment for an additional 6, 12, or 18 months, the cholangiofibrosis progressed to yield a 100% incidence of cholangiocarcinomas. Transplantation of 21 primary cholangiocarcinomas into syngeneic recipients resulted in growth from 4 donors. The 4 transplanted lines were successfully transferred through 8 serial passages and resulted in metastases in the recipients. The progressive growth of these proliferative hepatocholangial lesions over time, their transplantability, and the development of metastases in some of the cases provide biological evidence of the malignant potential of the furan-induced liver changes. RP MARONPOT, RR (reprint author), NIEHS,NATL TOXICOL PROGRAM,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 50 Z9 51 U1 0 U2 1 PU SOC TOXICOLOGIC PATHOLOGISTS PI LAWRENCE PA 1041 NEW HAMPSHIRE ST PO BOX 368, LAWRENCE, KS 66044 SN 0192-6233 J9 TOXICOL PATHOL JI Toxicol. Pathol. PY 1991 VL 19 IS 4 BP 561 EP 570 PN 2 PG 10 WC Pathology; Toxicology SC Pathology; Toxicology GA HQ750 UT WOS:A1991HQ75000001 PM 1668599 ER PT J AU WARD, JM HENNEMAN, JR OSIPOVA, GY ANISIMOV, VN AF WARD, JM HENNEMAN, JR OSIPOVA, GY ANISIMOV, VN TI PERSISTENCE OF 5-BROMO-2'-DEOXYURIDINE IN TISSUES OF RATS AFTER EXPOSURE IN EARLY LIFE SO TOXICOLOGY LA English DT Article DE 5-BROMO-2'-DEOXYURIDINE; IMMUNOHISTOCHEMISTRY; TOXICITY ID MONOCLONAL-ANTIBODY; DNA; 5-BROMODEOXYURIDINE; CARCINOGENESIS AB Outbred L10 rats received 4 subcutaneous injections of 3.2 mg per rat 5-bromo-2'-deoxyuridine (BrdUrd) at 1, 3, 7 and 21 days of age. Groups of rats were sacrificed 1 h, 1 month or 49 weeks after the final injection. Tissues were fixed in 70% ethanol, embedded in paraffin and unstained sections were prepared for immunohistochemical demonstration of BrdUrd. A monoclonal antibody to BrdUrd was used with the avidin biotin peroxidase-complex (ABC) technique. The numbers of immunoreactive nuclei were tissue, cell and time-related. The labeling indices declined from 1 day to 49 weeks for all tissues studied. At 49 weeks after the last exposure to BrdUrd, many cells were still reactive, especially in tissues with normal low cell turnover (brain, uterine stroma). For cells with high turnover, including lymphocytes and ovarian germinative epithelium, few or no labeled cells remained at 49 weeks. This study provides clear evidence for the persistence of BrdUrd in normal tissues, some of which may be targets for the carcinogenic effect of the chemical and others which are not targets. C1 NN PETROV ONCOL RES INST,EXPTL TUMOURS LAB,ST PETERSBURG 189646,USSR. NCI,FREDERICK CANC RES & DEV CTR,INC DYNCORP,PROGRAM RESOURCES,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21702. RP WARD, JM (reprint author), NCI,FREDERICK CANC RES & DEV CTR,COMPARAT CARCINOGENESIS LAB,TUMOR PATHOL & PATHOGENESIS SECT,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74102] NR 20 TC 19 Z9 19 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PY 1991 VL 70 IS 3 BP 345 EP 352 DI 10.1016/0300-483X(91)90008-O PG 8 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA GZ027 UT WOS:A1991GZ02700008 PM 1771641 ER PT J AU JANZ, S SHACTER, E AF JANZ, S SHACTER, E TI A NEW METHOD FOR DELIVERING ALKANES TO MAMMALIAN-CELLS - PREPARATION AND PRELIMINARY CHARACTERIZATION OF AN INCLUSION COMPLEX BETWEEN BETA-CYCLODEXTRIN AND PRISTANE (2,6,10,14-TETRAMETHYLPENTADECANE) SO TOXICOLOGY LA English DT Article DE 2,6,10,14-TETRAMETHYLPENTADECANE (PRISTANE); ALKANES; BETA-CYCLODEXTRIN; INCLUSION COMPLEX; PLASMACYTOMA ID RAT; CHROMATOGRAPHY; CARCINOGENESIS; LYMPHOCYTES; TOXICOLOGY; EXPRESSION; LIPOSOMES; VESICLES; PROTEIN; OILS AB Pristane (2,6,10,14-tetramethylpentadecane) is an isoalkane which induces plasma cell tumorigenesis in genetically susceptible strains of mice. Attempts to study the biological activity of pristane on cells in vitro have been hindered by the extreme hydrophobicity and hence complete immiscibility of the compound in aqueous cell culture media. In comparing different solubilization protocols such as using organic solvents, liposomes, and molecular encapsulation into beta-cyclodextrin (beta-CyD), it was found that beta-Cyd/pristane inclusion complexes were optimal for delivery of the hydrocarbon to cells. After solubilization in beta-CyD, pristane was cytotoxic (Cr-51 release assay) to murine B lymphocyte lines in culture (P388, NSF-1, and SJL-4) and inhibited the lipopolysaccharide-induced stimulation of splenic B lymphocyte proliferation and blast formation (Coulter counter analysis) when added in the micromolar concentration range. Moreover, sub-toxic concentrations of beta-CyD/pristane inclusion complexes were found to have a small but reproducible mitogenic effect ([H-3]thymidine incorporation) on SJL-4 and 308 (murine initiated keratinocyte) cells in culture but not on P388 cells. The results indicate that molecular encapsulation of pristane into beta-CyD provides a new and effective method for delivering low concentrations of alkanes to mammalian cells in vitro. RP JANZ, S (reprint author), NCI,GENET LAB,BLDG 37,RM 2B09,BETHESDA,MD 20892, USA. NR 51 TC 18 Z9 19 U1 0 U2 3 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PY 1991 VL 69 IS 3 BP 301 EP 315 DI 10.1016/0300-483X(91)90189-8 PG 15 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA GQ007 UT WOS:A1991GQ00700006 PM 1949053 ER PT J AU SOMKUTI, SG LAPADULA, DM CHAPIN, RE ABOUDONIA, MB AF SOMKUTI, SG LAPADULA, DM CHAPIN, RE ABOUDONIA, MB TI LIGHT AND ELECTRON-MICROSCOPIC EVIDENCE OF TRI-O-CRESYL PHOSPHATE (TOCP)-MEDIATED TESTICULAR TOXICITY IN FISCHER-344 RATS SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID INDUCED DELAYED NEUROTOXICITY; TRIMETHYLPHOSPHATE; PHOSPHORYLATION; DEGENERATION; MICROTUBULES; MALATHION; PROTEINS; DDVP; FSH C1 DUKE UNIV,MED CTR,DEPT PHARMACOL,POB 3813,DURHAM,NC 27710. DUKE UNIV,MED CTR,DEPT OBSTET & GYNECOL,DURHAM,NC 27710. NIEHS,NATL TOXICOL PROGRAM,FERTIL & REPROD GRP,RES TRIANGLE PK,NC 27709. OI Chapin, Robert/0000-0002-5997-1261 FU NIOSH CDC HHS [OH02003, R01 OH00823] NR 44 TC 22 Z9 24 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD JAN PY 1991 VL 107 IS 1 BP 35 EP 46 DI 10.1016/0041-008X(91)90328-C PG 12 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA ET039 UT WOS:A1991ET03900004 PM 1987658 ER PT J AU BIRNBAUM, LS MORRISSEY, RE HARRIS, MW AF BIRNBAUM, LS MORRISSEY, RE HARRIS, MW TI TERATOGENIC EFFECTS OF 2,3,7,8-TETRABROMODIBENZO-P-DIOXIN AND 3 POLYBROMINATED DIBENZOFURANS IN C57BL/6N MICE SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID POLYCHLORINATED DIBENZOFURANS; DEVELOPMENTAL TOXICITY; FLAME RETARDANTS; CLEFT-PALATE; PARA-DIOXINS; 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN; DISPOSITION; RAT; INDUCTION; PRODUCTS C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 31 TC 33 Z9 35 U1 1 U2 4 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD JAN PY 1991 VL 107 IS 1 BP 141 EP 152 DI 10.1016/0041-008X(91)90338-F PG 12 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA ET039 UT WOS:A1991ET03900014 PM 1987653 ER PT J AU TSUTSUI, T KAWAMOTO, Y SUZUKI, N GLADEN, BC BARRETT, JC AF TSUTSUI, T KAWAMOTO, Y SUZUKI, N GLADEN, BC BARRETT, JC TI CYTOTOXICITY AND CHROMOSOME-ABERRATIONS IN NORMAL HUMAN ORAL KERATINOCYTES INDUCED BY CHEMICAL CARCINOGENS - COMPARISON OF INTERINDIVIDUAL VARIATIONS SO TOXICOLOGY IN VITRO LA English DT Article ID SODIUM-FLUORIDE; INVITRO; CANCER; TRANSFORMATION; TOXICITY; DAMAGE; TONGUE AB Normal human keratinocytes from the oral cavity were cultured in vitro in serum-free medium. Cultures from different individuals were established, and the responses of the cells to different chemicals were compared. The cells, grown at clonal densities, were treated separately with an alkylating agent (N-methyl-N'-nitro-N-nitrosoguanidine; MNNG), two arsenical salts (sodium arsenate or sodium arsenite), sodium fluoride or two polyaromatic hydrocarbons (benzo[a]pyrene or 7,12-dimethylbenz[a]anthracene). There were no significant differences in the colony-forming efficiencies (22.8 +/- 4.2%) of control (untreated) cells from five different individuals. At selected doses, each of the chemicals reduced the colony-forming efficiencies of the treated cells. The cytotoxicity of most of the chemicals did not differ significantly among cells derived from different individuals, with the exception of sodium arsenate at two doses and sodium fluoride at the highest dose tested. Induction of chromosome abberations by MNNG, sodium arsenite, sodium arsenate and sodium fluoride was analysed with cells derived from up to nine individuals. There was little difference in the inducibilities of chromosome aberrations among cultured keratinocytes from different donors. Treatment of cells from nine donors with one dose of sodium fluoride revealed a statistically significant inter-individual variation. These findings provide a model system to study the effects of carcinogens on the target cells for oral cancers. The results can be compared with findings for cells from other epithelial tissues, since the culture conditions support the growth of keratinocytes regardless of origin. Little inter-individual variation was observed in the response of oral keratinocytes to the chemicals examined. C1 NIEHS,POB 12233,RES TRIANGLE PK,NC 27709. NIPPON DENT UNIV TOKYO,SCH DENT,DEPT PHARMACOL,CHIYODA KU,TOKYO 102,JAPAN. NR 24 TC 6 Z9 6 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0887-2333 J9 TOXICOL IN VITRO JI Toxicol. Vitro PY 1991 VL 5 IS 4 BP 353 EP 361 DI 10.1016/0887-2333(91)90013-4 PG 9 WC Toxicology SC Toxicology GA GB455 UT WOS:A1991GB45500012 PM 20732037 ER PT J AU ZETTERGREN, LD BOLDT, BW PETERING, DH GOODRICH, MS WEBER, DN ZETTERGREN, JG AF ZETTERGREN, LD BOLDT, BW PETERING, DH GOODRICH, MS WEBER, DN ZETTERGREN, JG TI EFFECTS OF PROLONGED LOW-LEVEL CADMIUM EXPOSURE ON THE TADPOLE IMMUNE-SYSTEM SO TOXICOLOGY LETTERS LA English DT Article ID RANA-PIPIENS; FROG; ANTIBODY; ONTOGENY; SURFACE; LYMPHOCYTES; RECEPTOR; CELLS AB Uninjected (Group I) and sheep erythrocyte (SRBC)-injected (Group II) Rana tadpoles were exposed to varying sublethal concentrations of cadmium (Cd) for 6 weeks. In order to assess possible effects on the tadpole immune system we determined pre-B, B-mu, and plasma cell (PC-mu) frequencies in liver and mesonephros of Group I larvae, and hemagglutinating antibody (HA) titers of Group II animals. Group I and Group II control animals were cultivated in water with no added Cd (0 ppm), while treatments were set at 0.1, 0.2, 0.4 and 0.8 ppm Cd. We found that (a) Cd appeared to stimulate a significant increase in the frequency of B-mu cells in animals treated at 0.4 and 0.8 ppm, (b) certain treated Group II larvae contained significantly increased amounts of HA in their serum, while their serum protein concentrations were not significantly different, and (c) there was a dose-related increase in tissue Cd levels in treated Group II larvae. Our observations suggest that chronic low-level exposure to Cd may (a) result in a slight increases in the frequency of B-mu cells in unimmunized animals, (b) increase immune responsiveness of immunized larvae, and (c) increase liver and mesonephros accumulations of Cd. Preliminary studies indicated that cytosolic Cd is associated with a protein which appears to be similar to mammalian metallothionein. C1 NIEHS,CTR MARINE & FRESHWATER BIOMED CORE,MILWAUKEE,WI. UNIV WISCONSIN,DEPT CHEM,MILWAUKEE,WI 53201. SC JOHNSON & SON INC,DEPT DERMAL RES,RACINE,WI 53403. RP ZETTERGREN, LD (reprint author), CARROLL COLL,DEPT BIOL,WAUKESHA,WI 53186, USA. FU NIEHS NIH HHS [ES 04184] NR 28 TC 12 Z9 12 U1 0 U2 7 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0378-4274 J9 TOXICOL LETT JI Toxicol. Lett. PD JAN PY 1991 VL 55 IS 1 BP 11 EP 19 DI 10.1016/0378-4274(91)90022-X PG 9 WC Toxicology SC Toxicology GA EV025 UT WOS:A1991EV02500002 PM 1998193 ER PT J AU HAHIN, R WANG, GK SHAPIRO, BI STRICHARTZ, G AF HAHIN, R WANG, GK SHAPIRO, BI STRICHARTZ, G TI ALTERATIONS IN SODIUM-CHANNEL GATING PRODUCED BY THE VENOM OF THE MARINE MOLLUSK CONUS-STRIATUS SO TOXICON LA English DT Article ID MYELINATED NERVE; IONIC CURRENTS; KINETICS; INACTIVATION; CONOTOXINS; SAXITOXIN; PEPTIDE; TOXIN; PH AB The action of the venom from the marine mollusc Conus striatus was studied using the voltage-clamp technique on myelinated nerve. Conus venom applied to an isolated node of Ranvier at 1.1 mu-g protein/ml produced repetitive firing of action potentials when the node was depolarized under current-clamp conditions. Venom application produced a leftword (depolarizing) shift in both the peak sodium current-voltage and the permeability-voltage relationships. A concomitant decrease in maximum peak current and permeability also occurred. The time course of sodium current decline (inactivation) was slowed at all voltages by the presence of venom. Venom treatment caused only a slight depolarizing shift (5 mV) in the voltage-dependence of steady-state Na inactivation. The closing to the resting state of previously activated Na channels, "deactivation", was judged from Na "tail" currents following membrane repolarization, and was slowed more than four-fold by venom treatment. The changes in Na channel gating produced by Conus striatus venom can best be described as a stabilization of the open state of the Na channel and a shift in the voltage dependence of the opening of Na channels. The slowing of both inactivation and deactivation of Na channels can be simulated by alterations in the rate constants of a five state Markov model. C1 HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,ANESTHESIA RES LABS,BOSTON,MA 02115. NATL INST GEN MED SCI,BETHESDA,MD 20892. RP HAHIN, R (reprint author), NO ILLINOIS UNIV,DEPT BIOL SCI,DE KALB,IL 60115, USA. NR 26 TC 2 Z9 2 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0041-0101 J9 TOXICON JI Toxicon PY 1991 VL 29 IS 2 BP 245 EP 259 DI 10.1016/0041-0101(91)90109-5 PG 15 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EX659 UT WOS:A1991EX65900012 PM 1646501 ER PT J AU MURATA, M GUSOVSKY, F SASAKI, M YOKOYAMA, A YASUMOTO, T DALY, JW AF MURATA, M GUSOVSKY, F SASAKI, M YOKOYAMA, A YASUMOTO, T DALY, JW TI EFFECT OF MAITOTOXIN ANALOGS ON CALCIUM INFLUX AND PHOSPHOINOSITIDE BREAKDOWN IN CULTURED-CELLS SO TOXICON LA English DT Article ID RAT PHEOCHROMOCYTOMA CELLS; POTENT MARINE TOXIN; GAMBIERDISCUS-TOXICUS; INOSITOL PHOSPHATES; GUINEA-PIG; CHANNEL; RELEASE; DINOFLAGELLATE; ACTIVATOR; CA-2+ AB Maitotoxin (MTX) and the analogues, bis-desulfated-MTX (didesulfo-MTX), monodesulfated-MTX (monodesulfo-MTX), and hydrogenated-MTX (H-MTX) were examined on Ca-45(2+) influx and phosphoinositide breakdown with hamster insulinoma HIT cells and rat glioma C6 cells. The activity of MTX was greatly reduced either by desulfation or by hydrogenation. Didesulfo-MTX weakly stimulated calcium influx in HIT cells, but had no stimulatory effect on either calcium influx or phosphoinositide breakdown in C6 cells. All the analogues inhibited MTX-induced calcium influx in either HIT or C6 cells. Didesulfo-MTX inhibited the calcium influx elicited by 3 ng/ml MTX in C6 cells with an IC50 of 7.0 +/- 0.7 ng/ml. The data suggest that the sulfate groups in MTX are important for stimulation of calcium influx and phosphoinositide breakdown, but are not essential for binding to a receptor-site on cell membranes. Although catalytic reduction of double bonds in MTX reduced activity by nearly 100-fold, a tritiated H-MTX still represents a potential radioligand for identification of MTX-binding sites. C1 NIDDK,BIOORGAN CHEM LAB,BLDG 8,ROOM 1A-15,BETHESDA,MD 20892. TOHOKU UNIV,FAC AGR,FOOD HYG LAB,SENDAI 981,JAPAN. OI murata, michio/0000-0002-1600-145X NR 33 TC 11 Z9 13 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0041-0101 J9 TOXICON JI Toxicon PY 1991 VL 29 IS 9 BP 1085 EP 1096 DI 10.1016/0041-0101(91)90206-7 PG 12 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA GJ400 UT WOS:A1991GJ40000003 PM 1665603 ER PT J AU MANNS, A BLATTNER, WA AF MANNS, A BLATTNER, WA TI THE EPIDEMIOLOGY OF THE HUMAN T-CELL LYMPHOTROPHIC VIRUS TYPE-I AND TYPE-II - ETIOLOGIC ROLE IN HUMAN-DISEASE SO TRANSFUSION LA English DT Review ID TROPICAL SPASTIC PARAPARESIS; LEUKEMIA-LYMPHOMA VIRUS; HTLV-I; BLOOD-TRANSFUSION; ACTIVATED LYMPHOCYTES; CHILD TRANSMISSION; HOMOSEXUAL MEN; UNITED-STATES; C RETROVIRUS; DRUG-ABUSERS C1 NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. NR 84 TC 142 Z9 144 U1 1 U2 3 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 SN 0041-1132 J9 TRANSFUSION JI Transfusion PD JAN PY 1991 VL 31 IS 1 BP 67 EP 75 DI 10.1046/j.1537-2995.1991.31191096189.x PG 9 WC Hematology SC Hematology GA ET293 UT WOS:A1991ET29300014 PM 1986467 ER PT J AU GEORGE, AJT AF GEORGE, AJT TI REGULATION OF THE B-CELL RESPONSE BY CYTOKINES SO TRANSFUSION SCIENCE LA English DT Article AB An ever increasing number of soluble mediators, or cytokines, have been described that are used by the immune system to regulate B lymphocyte function. These factors have an important role in the pathogenesis of a number of diseases, and an understanding of their action and behaviour is likely to prove important in the development of novel treatment protocols. In this review I discuss 8 such factors, the interleukins 1-7 and interferon-gamma, and their action on B cells. Their effects are dependent not only on the B cell itself, but also on the presence of other cytokines. The factors also have pleiotropic effects on other immune cells, making it clear that the immune response is controlled by a cytokine network whose complexity we are only just beginning to appreciate. RP GEORGE, AJT (reprint author), NCI,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4B17,BETHESDA,MD 20892, USA. OI George, Andrew/0000-0002-2866-0241 NR 0 TC 1 Z9 1 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0955-3886 J9 TRANSFUS SCI JI Transfus. Sci. PY 1991 VL 12 IS 1-2 BP 43 EP 57 DI 10.1016/0955-3886(91)90008-Q PG 15 WC Hematology SC Hematology GA FH925 UT WOS:A1991FH92500007 ER PT J AU KIRKMAN, RL SHAPIRO, ME CARPENTER, CB MCKAY, DB MILFORD, EL RAMOS, EL TILNEY, NL WALDMANN, TA ZIMMERMAN, CE STROM, TB AF KIRKMAN, RL SHAPIRO, ME CARPENTER, CB MCKAY, DB MILFORD, EL RAMOS, EL TILNEY, NL WALDMANN, TA ZIMMERMAN, CE STROM, TB TI A RANDOMIZED PROSPECTIVE TRIAL OF ANTI-TAC MONOCLONAL-ANTIBODY IN HUMAN RENAL-TRANSPLANTATION SO TRANSPLANTATION LA English DT Article; Proceedings Paper CT 16TH ANNUAL MEETING OF THE AMERICAN SOC OF TRANSPLANT SURGEONS CY MAY 30-JUN 01, 1990 CL CHICAGO, IL SP AMER SOC TRANSPLANT SURGEONS ID CARDIAC ALLOGRAFT SURVIVAL; HUMAN T-CELLS; INTERLEUKIN-2 RECEPTOR; EXPRESSION; PROLONGATION; RECIPIENTS; THERAPY; BINDS; MICE AB Patient entry is now complete in a prospective trial of anti-Tac, a murine IgG2a monoclonal antibody directed against the p55 chain of the human IL-2 receptor, for the prevention of renal allograft rejection. Recipients of primary cadaver allografts were randomized to receive either anti-Tac (20 mg q.d. x 10 days beginning POD 1) plus low-dose CsA (4 mg/kg/day), azathioprine (2 mg/kg/day), and prednisone (30 mg q.d.), or conventional triple therapy with CsA (8 mg/kg/day), azathioprine, and prednisone. Forty patients were entered in each group, with current followup from 6 to 26 months. The results show a significant reduction in early rejection episodes in the anti-Tac-treated patients. During the 10-day treatment, 5 of 40 anti-Tac patients had rejection episodes, compared with 21 of 40 control patients (P < 0.001). Anti-Tac significantly delayed the time to the first rejection (12.5 +/- 6.3 vs. 7.6 +/- 6.7 days) (P < 0.05). Despite these effects, there were no differences in either actual or actuarial graft or patient survival between the two groups. Pneumonia, primarily CMV, developed in 5 treated and 4 control patients. In patients with functioning grafts mean serum creatinine at 3 months was 1.8 +/- 0.7 in the anti-Tac group and 2.0 +/- 0.8 in the control group (P = NS); at 12 months the values were 2.3 +/- 1.5 and 1.8 +/- 0.5, respectively (P = NS). The peak expression of IL-2 receptors on circulating T-cells was significantly lower in anti-Tac patients (15.1 +/- 3.6%) than in controls (21.9 +/- 4.5%) (P < 0.05). Seven of 10 patients tested to date developed antimouse immunoglobulin antibodies, with antiidiotype shown in 6. These antibodies do not preclude subsequent treatment with OKT3. Five patients in this and previous anti-Tac protocols have received OKT3 for acute rejection despite known pretreatment antimouse antibodies, with resolution of rejection in all cases. C1 HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,BOSTON,MA 02115. NCI,METAB BRANCH,BETHESDA,MD 20892. RP KIRKMAN, RL (reprint author), BRIGHAM & WOMENS HOSP,DEPT SURG,75 FRANCIS ST,BOSTON,MA 02115, USA. OI Shapiro, Michael/0000-0001-7491-6490 FU NIAID NIH HHS [N01-AI-82512] NR 22 TC 129 Z9 134 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD JAN PY 1991 VL 51 IS 1 BP 107 EP 113 DI 10.1097/00007890-199101000-00016 PG 7 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EU056 UT WOS:A1991EU05600016 PM 1846250 ER PT J AU CARROLL, PB BOSCHERO, AC LI, MY TZAKIS, AG STARZL, TE ATWATER, I AF CARROLL, PB BOSCHERO, AC LI, MY TZAKIS, AG STARZL, TE ATWATER, I TI EFFECT OF THE IMMUNOSUPPRESSANT-FK506 ON GLUCOSE-INDUCED INSULIN-SECRETION FROM ADULT-RAT ISLETS OF LANGERHANS SO TRANSPLANTATION LA English DT Note ID PANCREATIC BETA-CELLS; CYCLOSPORIN-A; FK-506; RELEASE; TRANSPLANTATION; ALLOGRAFTS C1 UNIV PITTSBURGH,SCH MED,DEPT SURG,PITTSBURGH,PA 15261. RP CARROLL, PB (reprint author), NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD 20892, USA. RI Boschero, Antonio/O-7525-2014 OI Boschero, Antonio/0000-0003-3829-8570 FU NIDDK NIH HHS [R01 DK029961-19] NR 24 TC 33 Z9 33 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD JAN PY 1991 VL 51 IS 1 BP 275 EP 277 PG 3 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA EU056 UT WOS:A1991EU05600047 PM 1702911 ER PT J AU GARDNER, MB KOZAK, CA OBRIEN, SJ AF GARDNER, MB KOZAK, CA OBRIEN, SJ TI THE LAKE CASITAS WILD MOUSE - EVOLVING GENETIC-RESISTANCE TO RETROVIRAL DISEASE SO TRENDS IN GENETICS LA English DT Article ID MURINE LEUKEMIA-VIRUS; MUS-MUSCULUS-MOLOSSINUS; FV-4 RESISTANCE; NUCLEOTIDE-SEQUENCE; RESTRICTION GENE; ECOTROPIC VIRUS; MICE; SUSCEPTIBILITY; AKVR-1; DNA AB A small colony of feral mice from California continues to flourish in spite of a virulent epizootic of pathological retrovirus. Epidemiological and genetic studies revealed that the viral infection is strongly balanced by the polymorphic resistance locus, Fv-4, a transcriptionally active but truncated provirus that originated in the East Asian ancestors of the Californian mice. The natural history of these populations represents a graphic example of genomic adaptation in free-ranging populations to regulate and delimit infectious disease. C1 NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. NCI,FREDERICK CANC RES FACIL,OTOLARYNGOL LAB,FREDERICK,MD 21701. RP GARDNER, MB (reprint author), UNIV CALIF DAVIS,SCH MED,DEPT MED PATHOL,DAVIS,CA 95616, USA. NR 33 TC 70 Z9 70 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0168-9525 J9 TRENDS GENET JI Trends Genet. PD JAN PY 1991 VL 7 IS 1 BP 22 EP 27 DI 10.1016/0168-9525(91)90017-K PG 6 WC Genetics & Heredity SC Genetics & Heredity GA EP913 UT WOS:A1991EP91300006 PM 1848377 ER PT J AU GONZALES, RA HOFFMAN, PL AF GONZALES, RA HOFFMAN, PL TI RECEPTOR-GATED ION CHANNELS MAY BE SELECTIVE CNS TARGETS FOR ETHANOL SO TRENDS IN PHARMACOLOGICAL SCIENCES LA English DT Editorial Material ID INHIBITION C1 NIAAA,DIV INTRAMURAL CLIN & BIOL RES,BETHESDA,MD 20892. RP GONZALES, RA (reprint author), UNIV TEXAS,COLL PHARM,DEPT PHARMACOL,AUSTIN,TX 78712, USA. NR 15 TC 68 Z9 68 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0165-6147 J9 TRENDS PHARMACOL SCI JI Trends Pharmacol. Sci. PD JAN PY 1991 VL 12 IS 1 BP 1 EP 3 DI 10.1016/0165-6147(91)90478-B PG 3 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EV816 UT WOS:A1991EV81600001 PM 1706544 ER PT J AU JENSEN, RT COY, DH AF JENSEN, RT COY, DH TI PROGRESS IN THE DEVELOPMENT OF POTENT BOMBESIN RECEPTOR ANTAGONISTS SO TRENDS IN PHARMACOLOGICAL SCIENCES LA English DT Review ID GASTRIN-RELEASING PEPTIDE; CELL LUNG-CANCER; SWISS 3T3 CELLS; PANCREATIC ACINAR; GROWTH-FACTORS; NEUROMEDIN-B; ANALOGS; BACKBONE; SUBTYPES; BINDING AB Bombesin and the mammalian-related peptides gastrin-releasing peptide (GRP), GRP18-27 and neuromedin B have been shown to have numerous actions in the CNS, gastrointestinal tract and on growth. However, the role of the peptides in various physiological processes has remained unclear because of the lack of potent antagonists. Recent in vitro studies have described four different classes of bombesin receptor antagonist, some of which are active in the nanomolar range and in vivo. Robert Jensen and David Coy describe recent insights into peptide structural determinants of biological activity. Evidence from structure-function studies have resulted in identification of some analogues that function as potent antagonists in all systems examined. Furthermore, various subtypes of bombesin receptors can now be differentiated by these various classes of antagonist. C1 TULANE UNIV,MED CTR,NEW ORLEANS,LA 70112. RP JENSEN, RT (reprint author), NIDDKD,DIGEST DIS BRANCH,CELL BIOL SECT,BETHESDA,MD 20892, USA. FU NCI NIH HHS [CA 45153] NR 47 TC 159 Z9 160 U1 0 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0165-6147 J9 TRENDS PHARMACOL SCI JI Trends Pharmacol. Sci. PD JAN PY 1991 VL 12 IS 1 BP 13 EP 19 DI 10.1016/0165-6147(91)90483-9 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EV816 UT WOS:A1991EV81600006 PM 1706545 ER PT J AU MACLEOD, CB TSOKOS, M AF MACLEOD, CB TSOKOS, M TI GASTROINTESTINAL AUTONOMIC NERVE TUMOR SO ULTRASTRUCTURAL PATHOLOGY LA English DT Article DE GASTROINTESTINAL AUTONOMIC NERVE TUMOR; PLEXOSARCOMA; GASTROINTESTINAL STROMAL TUMOR ID SMOOTH-MUSCLE TUMORS; STROMAL TUMORS; GANGLIOCYTIC PARAGANGLIOMA; DUODENUM; ORIGIN; LIGHT; TRACT AB The article describes a case of gastrointestinal autonomic nerve tumor, which is histogenetically related to the gastrointestinal autonomic plexus (hence the name plexosarcoma). This rare and only recently recognized tumor of the gastrointestinal tract appears to have significant prognostic implications. This tumor cannot be diagnosed unequivocally by light microscopic and immunocytochemical examinations but shows characteristic electron microscopic features. The present case occurred as a gastric primary tumor and exhibited a light and electron microscopic picture similar to the one described in previous reports: areas of spindle-shaped and epithelioid cells, cytoplasmic processes with dense-core granules, and cytoplasmic intermediate filaments. Ultrastructural characteristics diagnostic of other gastrointestinal tumors, such as those originating from smooth muscle, Schwann cell, or endocrine cell types, were absent. Immunocytochemically, the tumor was diffusely positive for vimentin and neuron-specific enolase and focally positive for neurofilament triplet protein (NFTP) 160. Negative staining was observed for NFTP 200, S-100 protein, desmin, somatostatin, chromogranin, keratins (AE1/AE3), and glial fibrillary acidic protein. Although gastrointestinal autonomic nerve tumor has been reported to have a deceptively lowgrade malignant appearance by light microscopy, it follows an aggressive clinical course. This tumor showed a much higher mitotic rate (one mitosis per high-power field) than the rates of tumors reported previously. Moreover, it occurred in a much younger patient (20 years of age) compared to previously reported cases (45 to 66 years of age), with the exception of one other case (16 years of age). C1 NIH,ULTRASTRUCT PATHOL SECT,BETHESDA,MD 20892. NR 12 TC 33 Z9 33 U1 0 U2 0 PU HEMISPHERE PUBL CORP PI BRISTOL PA 1900 FROST ROAD, SUITE 101, BRISTOL, PA 19007-1598 SN 0191-3123 J9 ULTRASTRUCT PATHOL JI Ultrastruct. Pathol. PD JAN-FEB PY 1991 VL 15 IS 1 BP 49 EP 55 PG 7 WC Microscopy; Pathology SC Microscopy; Pathology GA EV627 UT WOS:A1991EV62700005 PM 1849328 ER PT J AU MOREIRA, JE HAND, AR BORG, LAH SANDLER, S WELSH, M WELSH, N EIZIRIK, DL AF MOREIRA, JE HAND, AR BORG, LAH SANDLER, S WELSH, M WELSH, N EIZIRIK, DL TI DECREASE IN INSULIN-CONTAINING SECRETORY GRANULES AND MITOCHONDRIAL GENE-EXPRESSION IN MOUSE PANCREATIC-ISLETS MAINTAINED IN CULTURE FOLLOWING STREPTOZOTOCIN EXPOSURE SO VIRCHOWS ARCHIV B-CELL PATHOLOGY INCLUDING MOLECULAR PATHOLOGY LA English DT Article DE STREPTOZOTOCIN; PANCREATIC ISLETS; INSULIN RELEASE; CYTOCHROME B MESSENGER-RNA; ULTRASTRUCTURE ID BETA-CELL DAMAGE; ULTRASTRUCTURAL LOCALIZATION; POLY(ADP-RIBOSE) SYNTHETASE; FUNCTIONAL-CHARACTERISTICS; MESSENGER-RNA; INVITRO; GLUCOSE; DNA; NITROSOUREA; HYBRIDIZATION AB We have previously described a preferential reduction in the secretory response to nutrient secretagogues in pancreatic mouse islets maintained in culture after in vitro exposure to streptozotocin (SZ). This reduction was associated with an impaired substrate metabolism at the mitochondrial level. To further clarify this issue, mouse pancreatic islets were exposed in vitro to 2.2 mM SZ for 30 min. At 4 h after SZ treatment ultrastructural changes were apparent in the endoplasmic reticulum and Golgi areas of the B-cells. However, 2 and 6 days following SZ exposure the B-cells appeared well preserved, except for a marked decrease in the number of insulin-containing secretory granules. A morphometric analysis of the B-cells 6 days after SZ exposure showed a normal B-cell size and a normal volume fraction of B-cell mitochondria. However, there was a decrease in total islet size and a 13% decrease in the volume fraction of B-cells in the islets. These mouse islets exhibited a decreased content of the mitochondrial DNA-encoded cytochrome b mRNA, as evaluated by dot-blot analysis. As a whole, the data obtained indicate that SZ treatment does not induce a decrease in the number of mitochondria or long-lasting ultrastructural damage to this organelle. However, there is a clear decrease in the cytochrome b mRNA, suggesting that SZ can induce damage to the mitochondrial DNA. C1 UNIV UPPSALA,BIOMEDICUM,DEPT MED CELL BIOL,S-75123 UPPSALA,SWEDEN. NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892. NR 36 TC 18 Z9 18 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-6075 J9 VIRCHOWS ARCH B JI Virchows Arch. B-Cell Molec. Pathol. PY 1991 VL 60 IS 5 BP 337 EP 344 DI 10.1007/BF02899565 PG 8 WC Pathology SC Pathology GA FY278 UT WOS:A1991FY27800008 PM 1685041 ER PT J AU AMEGADZIE, BY HOLMES, MH COLE, NB JONES, EV EARL, PL MOSS, B AF AMEGADZIE, BY HOLMES, MH COLE, NB JONES, EV EARL, PL MOSS, B TI IDENTIFICATION, SEQUENCE, AND EXPRESSION OF THE GENE ENCODING THE 2ND-LARGEST SUBUNIT OF THE VACCINIA VIRUS DNA-DEPENDENT RNA-POLYMERASE SO VIROLOGY LA English DT Article ID ANTIBODY PROBES; GENOME; TRANSCRIPTION; FRAGMENTS; VIRIONS; TRANSLATION; TERMINATION; INITIATION; CLONING C1 NIAID,VIRAL DIS LAB,BETHESDA,MD 20892. NR 41 TC 40 Z9 42 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD JAN PY 1991 VL 180 IS 1 BP 88 EP 98 DI 10.1016/0042-6822(91)90012-Z PG 11 WC Virology SC Virology GA EP908 UT WOS:A1991EP90800011 PM 1824607 ER PT J AU HIZI, A TAL, R HUGHES, SH AF HIZI, A TAL, R HUGHES, SH TI MUTATIONAL ANALYSIS OF THE DNA-POLYMERASE AND RIBONUCLEASE-H ACTIVITIES OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2 REVERSE-TRANSCRIPTASE EXPRESSED IN ESCHERICHIA-COLI SO VIROLOGY LA English DT Article ID RNASE-H; INSERTION MUTAGENESIS; DOMAIN-STRUCTURE; AIDS; 3'-AZIDO-3'-DEOXYTHYMIDINE; ORGANIZATION; POLYPROTEIN; ENZYME C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21702. RP HIZI, A (reprint author), TEL AVIV UNIV,SACKLER SCH MED,DEPT CELL BIOL & HISTOL,TEL AVIV,ISRAEL. FU NCI NIH HHS [N01-CO-74101]; NIAID NIH HHS [R01-AI27035] NR 33 TC 74 Z9 75 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD JAN PY 1991 VL 180 IS 1 BP 339 EP 346 DI 10.1016/0042-6822(91)90038-D PG 8 WC Virology SC Virology GA EP908 UT WOS:A1991EP90800037 PM 1701948 ER PT J AU PALUMBO, GJ BULLER, RML AF PALUMBO, GJ BULLER, RML TI INHIBITORS OF THE LIPOXYGENASE PATHWAY SPECIFICALLY BLOCK ORTHOPOXVIRUS REPLICATION SO VIROLOGY LA English DT Note ID COWPOX VIRUS; VACCINIA; BIOGENESIS; INACTIVATION; MECHANISM; VARIANTS; PROTEIN; GENOME C1 NIAID,VIRAL DIS LAB,BETHESDA,MD 20892. NR 32 TC 11 Z9 11 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD JAN PY 1991 VL 180 IS 1 BP 457 EP 463 DI 10.1016/0042-6822(91)90058-J PG 7 WC Virology SC Virology GA EP908 UT WOS:A1991EP90800057 PM 1898625 ER PT J AU MYSLOBODSKY, MS GLICKSOHN, J COPPOLA, R WEINBERGER, DR AF MYSLOBODSKY, MS GLICKSOHN, J COPPOLA, R WEINBERGER, DR TI OCCIPITAL LOBE MORPHOLOGY IN NORMAL INDIVIDUALS ASSESSED BY MAGNETIC-RESONANCE-IMAGING (MRI) SO VISION RESEARCH LA English DT Article DE VISUAL EVOKED POTENTIAL; MAGNETIC RESONANCE IMAGING; HEMISPHERIC ASYMMETRY; OCCIPITAL LOBE MORPHOLOGY ID INTERNATIONAL 10-20 SYSTEM; EEG ELECTRODES; TOPOGRAPHY; LOCALIZATION; VEPS AB The topography of visual evoked potentials (VEP) is dependent on occipital lobe morphology. Using magnetic resonance imaging we examine the sulcal pattern (the calcarine and parieto-occipital sulci), and assess the size of the cuneus and the asymmetry of the occipital lobes, computed separately for its ventral and dorsal segments. No differences were found for either the cuneus or the sulci pattern. In contrast, hemispheric asymmetry values appeared to be substantial. The predominance of the left occipital area was seen distinctly in the ventro-caudal portion of the occipital lobe. It was frequently reversed in the dorsal aspect of the lobe, notably in more rostral cuts. Such complexities may lead to ambiguities in interpreting VEP asymmetries. C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. RP MYSLOBODSKY, MS (reprint author), TEL AVIV UNIV,PSYCHOBIOL RES UNIT,IL-69978 TEL AVIV,ISRAEL. NR 25 TC 12 Z9 13 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0042-6989 J9 VISION RES JI Vision Res. PY 1991 VL 31 IS 10 BP 1677 EP 1685 DI 10.1016/0042-6989(91)90019-2 PG 9 WC Neurosciences; Ophthalmology SC Neurosciences & Neurology; Ophthalmology GA GB262 UT WOS:A1991GB26200003 PM 1767490 ER PT B AU HERKENHAM, M AF HERKENHAM, M BE FUXE, K AGNATI, LF TI MISMATCHES BETWEEN NEUROTRANSMITTER AND RECEPTOR LOCALIZATIONS - IMPLICATIONS FOR ENDOCRINE FUNCTIONS IN BRAIN SO VOLUME TRANSMISSION IN THE BRAIN: NOVEL MECHANISMS FOR NEURAL TRANSMISSION SE ADVANCES IN NEUROSCIENCE LA English DT Proceedings Paper CT INTERNATIONAL SYMP ON VOLUME TRANSMISSION IN THE BRAIN : EXTRACELLULAR FLUID AS PATHWAYS FOR ELECTRICAL AND CHEMICAL COMMUNICATION CY SEP 28-30, 1989 CL STOCKHOLM, SWEDEN SP WENNER GREN CTR RP HERKENHAM, M (reprint author), NIMH,FUNCT NEUROANAT SECT,CLIN NEUROENDOCRINOL BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 18 Z9 19 U1 0 U2 0 PU RAVEN PRESS PI NEW YORK PA NEW YORK BN 0-88167-699-3 J9 ADV NEUROSC PY 1991 VL 1 BP 63 EP 87 PG 25 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA BS56P UT WOS:A1991BS56P00006 ER PT J AU SHARPE, PA CLARK, NM JANZ, NK AF SHARPE, PA CLARK, NM JANZ, NK TI DIFFERENCES IN THE IMPACT AND MANAGEMENT OF HEART-DISEASE BETWEEN OLDER WOMEN AND MEN SO WOMEN & HEALTH LA English DT Article ID SEX-DIFFERENCES; DRUG-USE; HEALTH-STATUS; MORTALITY; PROFILE; SURGERY RP SHARPE, PA (reprint author), UNIV MICHIGAN,NATL INST AGING,SCH PUBL HLTH,DEPT HLTH BEHAV & HLTH EDUC,ANN ARBOR,MI 48109, USA. FU NHLBI NIH HHS [5-R01-HL38083-02] NR 39 TC 43 Z9 44 U1 2 U2 5 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 0363-0242 J9 WOMEN HEALTH JI Women Health PY 1991 VL 17 IS 2 BP 25 EP 43 DI 10.1300/J013v17n02_02 PG 19 WC Public, Environmental & Occupational Health; Women's Studies SC Public, Environmental & Occupational Health; Women's Studies GA FQ090 UT WOS:A1991FQ09000002 PM 1871988 ER PT J AU NORTON, JA JENSEN, RT AF NORTON, JA JENSEN, RT TI UNRESOLVED SURGICAL ISSUES IN THE MANAGEMENT OF PATIENTS WITH ZOLLINGER-ELLISON SYNDROME SO WORLD JOURNAL OF SURGERY LA English DT Review ID MULTIPLE ENDOCRINE NEOPLASIA; GASTRIC CARCINOID-TUMORS; ISLET-CELL TUMORS; LOCALIZE GASTRINOMAS; MALIGNANT GASTRINOMA; PANCREATIC TUMORS; TNM SYSTEM; RESECTION; OMEPRAZOLE; SURGERY AB In the last 10 years, there have been a number of major advances that have markedly changed the management of patients with Zollinger-Ellison syndrome. These advances have been described recently in a number of excellent reviews [1-3]. After reading each of these reviews, including our own, one is left generally with the impression that most of the important problems and particularly those involving surgery in the treatment of this disease have been resolved; however, in considering the possible problems to address in protocols in the coming years in our patients with Zollinger-Ellison syndrome followed at the National Institutes of Health, it became apparent to us that not only will surgery play an increasingly important role in the management of this disease, but that there are a number of major questions in which various aspects involving surgery are not resolved. Furthermore, resolution of a number of these areas will have applicability to the surgical treatment of other islet cell tumors. The purpose of this article is to call attention to these questions with the hope that other investigators may also consider these and specifically attempt to address some of these issues in the surgical treatment of Zollinger-Ellison syndrome in prospective studies. C1 NIDDKD,DIGEST DIS BRANCH,CELL BIOL SECT,BETHESDA,MD. RP NORTON, JA (reprint author), NCI,SURG BRANCH,SURG METAB SECT,BLDG 10,ROOM 2B07,BETHESDA,MD 20892, USA. NR 105 TC 56 Z9 57 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0364-2313 J9 WORLD J SURG JI World J.Surg. PD JAN-FEB PY 1991 VL 15 IS 1 BP 151 EP 159 PG 9 WC Surgery SC Surgery GA FB902 UT WOS:A1991FB90200027 PM 1671617 ER PT J AU AYRES, TJ HERRMANN, DJ WIGGERT, H AF AYRES, TJ HERRMANN, DJ WIGGERT, H TI THE SPECIFICITY OF THE RETRIEVAL-PROCESSES INDUCED BY CATEGORY RETRIEVAL PRACTICE SO ZEITSCHRIFT FUR PSYCHOLOGIE LA English DT Article DE SEMANTIC MEMORY; RETRIEVAL PROCESSES ID SEMANTIC MEMORY; RECALL; HYPERMNESIA AB The present experiment investigates the effects of practice at retrieval of examples of categories in semantic memory. Subjects were engaged in one of three kinds of practice; over a series of trials, practice involved recall of: a single category (called specific practice); all different categories (called general practice); or repeated trials for each of three different categories (called blocked practice). One day after practice, subjects performed a transfer task involving a series of trials with a new category. Over the practice trials, recall increased substantially for specific and blocked practice, but only slightly for general practice. On the first transfer trial, a small amount of transfer was found for all three kinds of practice, indicating that all three induced a small but general improvement in the ability to recall any category. From the first through the fifth transfer trial, recall increased by an amount that was comparable to the first five trials of specific practice on the first day but in a manner that was the same for these three forms of practice. Apparently the kind of practice on the first day did not facilitate the increases in recall from specific practice on the second day. Overall, the results indicate that the greatest gains in the amount retrieved from a category in semantic memory come from specific practice. C1 NIMH,SOCIOENVIRONM STUDIES LAB,BETHESDA,MD 20892. COLGATE UNIV,HAMILTON,NY 13346. NR 27 TC 0 Z9 0 U1 0 U2 0 PU JOHANN AMBROSIUS BARTH VERLAG PI HEIDELBERG PA IM WEIHER 10, D-69121 HEIDELBERG, GERMANY SN 0044-3409 J9 Z PSYCHOL JI Z. Psychol. PY 1991 VL 199 IS 4 BP 309 EP 318 PG 10 WC Psychology, Multidisciplinary SC Psychology GA HD497 UT WOS:A1991HD49700005 ER PT J AU ONEILL, PL NOVAK, MA SUOMI, SJ AF ONEILL, PL NOVAK, MA SUOMI, SJ TI NORMALIZING LABORATORY-REARED RHESUS MACAQUE (MACACA-MULATTA) BEHAVIOR WITH EXPOSURE TO COMPLEX OUTDOOR ENCLOSURES SO ZOO BIOLOGY LA English DT Article DE ENRICHMENT; TRANSLOCATION; SURROGATE-PEER-REARED AB In comparison with standard laboratory settings, naturalistic environments typically provide nonhuman primates with increased visual, olfactory, and auditory stimulation and greater opportunities for exploration and manipulation. Although behavioral differences between monkeys reared in the laboratory and their feral counterparts are relatively well documented, less is known about the impact of transferring laboratory-born and -reared primates to outdoor settings. Of particular relevance is whether lab-reared primates retain the ability to respond in a species-specific manner to environments designed to mimic certain features of natural habitats and if short-term transfers have beneficial consequences. The present study compared the behavior of two groups of laboratory-born juvenile rhesus monkeys reared in an identical manner. One group was then transferred to an outdoor, apparatus-enriched corncrib adjacent to a semiwooded pasture. The other group remained indoors in a standard laboratory pen. Both groups were observed for 6 weeks prior to the transfer, during the 9-week period of transfer, and for 6 weeks after the outdoor animals were returned to their laboratory cage. During their outdoor experience, corncrib-housed subjects responded with higher locomotion and exploration scores. Animals housed in the outdoor corncrib also showed a decline in self-oral behavior which persisted even upon return to the laboratory environment. These data suggest that juvenile monkeys can benefit from brief exposure to enriched outdoor settings. RP ONEILL, PL (reprint author), NIH,CTR ANIM,22765 CLUB HOLLOW RD,DICKERSON,MD 20842, USA. NR 0 TC 24 Z9 25 U1 3 U2 14 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0733-3188 J9 ZOO BIOL JI Zoo Biol. PY 1991 VL 10 IS 3 BP 237 EP 245 DI 10.1002/zoo.1430100307 PG 9 WC Veterinary Sciences; Zoology SC Veterinary Sciences; Zoology GA FQ615 UT WOS:A1991FQ61500006 ER PT J AU KOREFSANTIBANEZ, S PAEPKE, HJ TJIO, HJ AF KOREFSANTIBANEZ, S PAEPKE, HJ TJIO, HJ TI KARYOTYPES OF THE SPECIES OF THE GENUS MACROPODUS LAC (PISCES, ANABANTOIDEI) SO ZOOLOGISCHER ANZEIGER LA German DT Article DE KARYOTYPES; FISHES; GENUS MACROPODUS ID FISHES; CHROMOSOMES; EVOLUTION; WATER AB The karyotypes of the following three species of Paradise-fish (Macropods), that form a monophyletic group within the Anabantoidei: Macropodus concolor, Macropodus ocellatus and Macropodus opercularis, were examined. All three species have a diploid chromosome set of 46; no major morphological differences were found between the karyotypes of M. ocellatus and M. opercularis (8 pairs M-SM; 15 pairs ST-T), while the chromosome set of M. concolor could be considered to have a more primitive structure (6 M-SM; 17 ST-T). Osphronemus goramy, a distantly related tropical Teleost has 48 chromosomes, including only one pair of submetacentrics and 46 telocentric chromosomes. C1 HUMBOLDT UNIV,MUSEUM NAT,INST SPEZIELLE ZOOL,O-1040 BERLIN,GERMANY. NIH,DEPT HLTH & HUMAN SERV,BETHESDA,MD 20892. RP KOREFSANTIBANEZ, S (reprint author), HUMBOLDT UNIV,ZOOL MUSEUM,INVALIDENSTR 43,O-1040 BERLIN,GERMANY. NR 18 TC 1 Z9 2 U1 0 U2 0 PU GUSTAV FISCHER VERLAG PI JENA PA VILLENGANG 2, D-07745 JENA, GERMANY SN 0044-5231 J9 ZOOL ANZ JI Zool. Anz. PY 1991 VL 227 IS 5-6 BP 271 EP 278 PG 8 WC Zoology SC Zoology GA HM641 UT WOS:A1991HM64100003 ER PT J AU BACHEVALIER, J AF BACHEVALIER, J TI ONTOGENIC DEVELOPMENT OF HABIT AND MEMORY FORMATION IN PRIMATES SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID RHESUS-MONKEYS; DISCRIMINATION; HIPPOCAMPUS; INFANT; RECOGNITION; RETENTION RP BACHEVALIER, J (reprint author), NIMH, NEUROPSYCHOL LAB, BETHESDA, MD 20892 USA. NR 62 TC 112 Z9 112 U1 0 U2 7 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 SN 0077-8923 J9 ANN NY ACAD SCI JI Ann. N.Y. Acad. Sci. PD DEC 31 PY 1990 VL 608 BP 457 EP 484 DI 10.1111/j.1749-6632.1990.tb48906.x PG 28 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM711 UT WOS:A1990FM71100018 PM 2127516 ER PT J AU FAGARASAN, MO AXELROD, J CATT, KJ AF FAGARASAN, MO AXELROD, J CATT, KJ TI INTERLEUKIN-1 POTENTIATES AGONIST-INDUCED SECRETION OF BETA-ENDORPHIN IN ANTERIOR-PITUITARY-CELLS SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID LINE ATT-20; RELEASE; CORTICOTROPHS; RECEPTORS C1 NICHHD,ENDOCRINOL & REPROD RES BRANCH,BETHESDA,MD 20892. RP FAGARASAN, MO (reprint author), NIMH,CELLULAR BIOL LAB,BETHESDA,MD 20892, USA. NR 11 TC 7 Z9 7 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD DEC 31 PY 1990 VL 173 IS 3 BP 988 EP 993 DI 10.1016/S0006-291X(05)80883-6 PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EQ577 UT WOS:A1990EQ57700031 PM 2268359 ER PT J AU PILARO, AM SAYERS, TJ MCCORMICK, KL REYNOLDS, CW WILTROUT, RH AF PILARO, AM SAYERS, TJ MCCORMICK, KL REYNOLDS, CW WILTROUT, RH TI AN IMPROVED INVITRO ASSAY TO QUANTITATE CHEMOTAXIS OF RAT PERIPHERAL-BLOOD LARGE ANTIGRANULOCYTES LYMPHOCYTES (LGL) SO JOURNAL OF IMMUNOLOGICAL METHODS LA English DT Article DE CHEMOTAXIS; LYMPHOCYTE MIGRATION; LARGE ANTIGRANULOCYTES LYMPHOCYTE, RAT ID MOUSE PERITONEAL-MACROPHAGES; PROTEIN-KINASE-C; NK CELL-ACTIVITY; MONOCLONAL-ANTIBODIES; EFFECTOR-CELLS; KILLER CELLS; COLLAGEN; FIBRONECTIN; RECEPTORS; INDUCTION AB We have developed an improved method to study the directed migration, or chemotaxis, of rat peripheral blood large granular lymphocytes (LGL) in vitro. A modified Boyden chamber technique was used to measure chemotaxis of LGL through pholycarbonate filters that had been coated with different basement membrane components. LGL were found to adhere to collagen types I and IV, laminin and fibronectin. However, only collagen type IV was not in itself chemotactic for LGL. Migrated cells could be identified both morphologically and phenotypically as LGL on collagen type IV-coated filters after incubation with a chemotactic stimulus. LGL were found to display chemotaxis to a number of different stimuli, including the classical chemoattractant agents N-formyl-methionyl-leucyl-phenylalanine, leukotriene B4, and complement fragments present in activated sera. However, the degree of response to these stimuli was much less than that of isolated peripheral blood neutrophils or monocytes. In contrast, all three cell types showed increased chemotaxis to the diacyl glycerol analog 1-oleoyl 2-acetyl glycerol (OAG), which induced a 4-14 fold stimulation of migration. Induction of chemotaxis of LGL by OAG was time and dose-dependent, as confirmed using checkerboard assays. In summary, we have developed a rapid, quantitative method to measure chemotaxis of LGL in vitro. This technique may now be utilized to identify naturally occurring chemoattractants for LGL and to study the intracellular and regulatory events associated with LGL migration. C1 NCI,FREDERICK CANC RES FACIL,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. RP PILARO, AM (reprint author), NCI,FREDERICK CANC RES FACIL,BIOL RESPONSE MODIFIERS PROGRAM,EXPTL IMMUNOL LAB,FREDERICK,MD 21701, USA. RI Sayers, Thomas/G-4859-2015 NR 46 TC 30 Z9 33 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1759 J9 J IMMUNOL METHODS JI J. Immunol. Methods PD DEC 31 PY 1990 VL 135 IS 1-2 BP 213 EP 224 DI 10.1016/0022-1759(90)90275-Z PG 12 WC Biochemical Research Methods; Immunology SC Biochemistry & Molecular Biology; Immunology GA EP295 UT WOS:A1990EP29500027 PM 2177075 ER PT J AU SORRENTINO, BP NEY, PA NIENHUIS, AW AF SORRENTINO, BP NEY, PA NIENHUIS, AW TI LOCALIZATION AND CHARACTERIZATION OF THE DNASE I-HYPERSENSITIVE SITE-II (HS-II) ENHANCER - A CRITICAL REGULATORY ELEMENT WITHIN THE BETA-GLOBIN LOCUS ACTIVATING REGION SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID PORPHOBILINOGEN DEAMINASE GENE; DOMINANT CONTROL REGION; HUMAN-LEUKEMIA CELLS; TRANSGENIC MICE; ERYTHROID PROMOTER; EXPRESSION; TRANSCRIPTION; HEMIN; BIND; INDUCTION RP SORRENTINO, BP (reprint author), NHLBI, CLIN HEMATOL BRANCH, BLDG 10 7C-210, 9000 ROCKVILLE PIKE, BETHESDA, MD 20892 USA. NR 31 TC 0 Z9 0 U1 2 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 28 PY 1990 VL 612 BP 141 EP 151 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FB934 UT WOS:A1990FB93400016 ER PT J AU BODINE, DM MCDONAGH, KT SEIDEL, NE NIENHUIS, AW AF BODINE, DM MCDONAGH, KT SEIDEL, NE NIENHUIS, AW TI DEVELOPMENT OF A HIGH-TITER RETROVIRUS PRODUCER CELL-LINE AND STRATEGIES FOR RETROVIRUS-MEDIATED GENE-TRANSFER INTO RHESUS-MONKEY HEMATOPOIETIC STEM-CELLS SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID HUMAN ADENOSINE-DEAMINASE; BETA-GLOBIN GENE; COLONY-STIMULATING FACTOR; LONG-TERM EXPRESSION; MICE; THERAPY; VECTORS; MOUSE; MODEL; SAFE RP BODINE, DM (reprint author), NHLBI, CLIN HEMATOL BRANCH, 9000 ROCKVILLE PIKE, BLDG 10, RM 7C103, BETHESDA, MD 20892 USA. NR 34 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 28 PY 1990 VL 612 BP 415 EP 426 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FB934 UT WOS:A1990FB93400045 ER PT J AU WU, KK PAPP, AC PATSCH, W ROCK, R ECKFELDT, J SHARRETT, R AF WU, KK PAPP, AC PATSCH, W ROCK, R ECKFELDT, J SHARRETT, R TI ARIC HEMOSTASIS STUDY .2. ORGANIZATIONAL PLAN AND FEASIBILITY STUDY SO THROMBOSIS AND HAEMOSTASIS LA English DT Article AB In our previous paper, we reported the development of a blood collection and processing system (BCPS) suitable for the ARIC multicenter hemostasis study. As an additional step of preparation for the ARIC study, we incorporated this BCPS into an organizational plan to increase efficiency and minimize errors. We initially designed organizational trays for blood collection tubes and aliquot tubes and developed a coordinated step-by-step plan for the orderly processing of blood samples. Once the plan was considered workable, we carried out a pilot study to test the feasibility of this integrated organizational plan. Included in the pilot study were 95 healthy subjects randomly selected from 4 ARIC field centers, whose age and gender were comparable to those projected for the ARIC population. We determined the time lapse of filling the first tube as an index of blood flow. The overall mean time-lapse was 23 s (S.D. = 5). There was no significant difference among the field centers. We also determined the entire time lapse required for completing the sample processing. The total processing time was always less than 60 min. By performing the processing of samples in pairs, all the samples from two subjects could be completely processed in 70 min. This greatly increased the efficiency of field center operation. We evaluated the potential in vitro hemostasis activation by measuring plasma beta-thromboglobulin and platelet factor 4 levels. The geometric means of both proteins were comparable to our previously reported results. Fibrinogen, factor VII, factor VIII, von Willebrand factor, antithrombin III, protein C and activated partial thromboplastin time were analyzed. All the hemostatic measurements exhibited no shift of the values. We conclude that the organizational plan together with the BCPS facilitate the incorporation of hemostasis measurements into the ARIC study. It should be valuable as a general guide for other multicenter studies measuring hemostatic factors. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED & PATHOL,DIV HEMATOL,HOUSTON,TX 77030. BAYLOR UNIV,DEPT MED,HOUSTON,TX 77030. JOHNS HOPKINS UNIV,SCH MED,DEPT LAB MED,BALTIMORE,MD 21205. UNIV MINNESOTA,SCH MED,DEPT LAB MED & PATHOL,MINNEAPOLIS,MN 55455. NHLBI,BETHESDA,MD 20892. RP WU, KK (reprint author), UNIV TEXAS,HLTH SCI CTR,VASC DIS RES CTR,6431 FANNIN,HOUSTON,TX 77030, USA. RI Wu, Kenneth Kun-Yu/B-1070-2010 FU NHLBI NIH HHS [HL-55022] NR 8 TC 32 Z9 32 U1 0 U2 1 PU F K SCHATTAUER VERLAG GMBH PI STUTTGART PA P O BOX 10 45 45, LENZHALDE 3, D-70040 STUTTGART, GERMANY SN 0340-6245 J9 THROMB HAEMOSTASIS JI Thromb. Haemost. PD DEC 28 PY 1990 VL 64 IS 4 BP 521 EP 525 PG 5 WC Hematology; Peripheral Vascular Disease SC Hematology; Cardiovascular System & Cardiology GA ET846 UT WOS:A1990ET84600006 PM 2084937 ER PT J AU CUTLER, GB ROSEN, H BLANKSEGAL, M MAJZOUB, J LAUE, L AF CUTLER, GB ROSEN, H BLANKSEGAL, M MAJZOUB, J LAUE, L TI CONGENITAL ADRENAL-HYPERPLASIA DUE TO 21-HYDROXYLASE DEFICIENCY SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Discussion ID MINERALOCORTICOID THERAPY; PRECOCIOUS PUBERTY; LINEAR GROWTH; EXPERIENCE; MATURATION; FERTILITY; HEIGHT; SECRETION; ESTRADIOL; PLASMA RP CUTLER, GB (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,BLDG 10,RM 10-N-262,BETHESDA,MD 20892, USA. NR 54 TC 60 Z9 61 U1 0 U2 2 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD DEC 27 PY 1990 VL 323 IS 26 BP 1806 EP 1813 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA EP376 UT WOS:A1990EP37600005 PM 2247119 ER PT J AU STGEORGIEV, V MCGOWAN, JJ AF STGEORGIEV, V MCGOWAN, JJ TI ACQUIRED-IMMUNE-DEFICIENCY-SYNDROME (AIDS) - PROGRESS IN DRUG RESEARCH AND THERAPEUTIC POTENTIAL SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID T-CELLS; HIV-1; PROTEIN C1 PENNWALT CORP, PHILADELPHIA, PA USA. NIAID, DIV AIDS, BASIC RES & DEV PROGRAM, BETHESDA, MD 20892 USA. NR 8 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 1 EP 10 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900001 ER PT J AU RAUSCH, DM HWANG, KM PADGETT, M VOLTZ, AH RIVAS, A ENGLEMAN, E GASTON, I MCGRATH, M FRASER, B KALYANARAMAN, VS NARA, PL DUNLOP, N MARTIN, L MURPHEYCORB, M KIBORT, T LIFSON, JD EIDEN, LE AF RAUSCH, DM HWANG, KM PADGETT, M VOLTZ, AH RIVAS, A ENGLEMAN, E GASTON, I MCGRATH, M FRASER, B KALYANARAMAN, VS NARA, PL DUNLOP, N MARTIN, L MURPHEYCORB, M KIBORT, T LIFSON, JD EIDEN, LE TI PEPTIDES DERIVED FROM THE CDR3-HOMOLOGOUS DOMAIN OF THE CD4 MOLECULE ARE SPECIFIC INHIBITORS OF HIV-1 AND SIV INFECTION, VIRUS-INDUCED CELL-FUSION, AND POSTINFECTION VIRAL TRANSMISSION INVITRO - IMPLICATIONS FOR THE DESIGN OF SMALL-PEPTIDE ANTI-HIV THERAPEUTIC AGENTS SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; IMMUNE-DEFICIENCY SYNDROME; RECOMBINANT SOLUBLE CD4; PNEUMOCYSTIS-CARINII PNEUMONIA; RETROVIRUS HTLV-III/LAV; HUMAN PERIPHERAL-BLOOD; T-CELL; MONOCLONAL-ANTIBODY; SYNCYTIUM FORMATION; AIDS VIRUS C1 NIMH, CELL BIOL LAB, BLDG 36, ROOM 3A-17, BETHESDA, MD 20892 USA. GENELABS INC, REDWOOD CITY, CA USA. STANFORD UNIV, CTR BLOOD, PALO ALTO, CA 94304 USA. UNIV CALIF SAN FRANCISCO, SAN FRANCISCO GEN HOSP, SAN FRANCISCO, CA 94110 USA. CTR BIOL EVALUAT & RES FOOD & DRUG ADM, BETHESDA, MD USA. BIONET RES LABS INC, ROCKVILLE, MD USA. NCI, TUMOR CELL BIOL LAB, FREDERICK, MD 21701 USA. TULANE UNIV, DELTA PRIMATE CTR, COVINGTON, LA 70433 USA. NR 80 TC 1 Z9 1 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 125 EP 148 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900013 ER PT J AU ASHORN, P MOSS, B BERGER, EA AF ASHORN, P MOSS, B BERGER, EA TI ANTI-HIV EFFECTS OF CD4-PSEUDOMONAS EXOTOXIN ON HUMAN LYMPHOCYTE AND MONOCYTE MACROPHAGE CELL-LINES SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; PSEUDOMONAS EXOTOXIN; INFECTED-CELLS; SOLUBLE CD4; HYBRID PROTEIN; AIDS VIRUS; FORM; AERUGINOSA; MOLECULES; RECEPTOR C1 NIAID, VIRAL DIS LAB, BETHESDA, MD 20892 USA. NR 25 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 149 EP 154 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900014 ER PT J AU ROSSI, JJ CANTIN, EM ZAIA, JA LADNE, PA CHEN, J STEPHENS, DA SARVER, N CHANG, PS AF ROSSI, JJ CANTIN, EM ZAIA, JA LADNE, PA CHEN, J STEPHENS, DA SARVER, N CHANG, PS TI RIBOZYMES AS THERAPIES FOR AIDS SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID INTERVENING SEQUENCE RNA; DELTA-VIRUS-RNA; SELF-CLEAVAGE; GENE; TETRAHYMENA; EXPRESSION; LIGATION; ENZYMES; SYSTEM; HIV-1 C1 CITY HOPE NATL MED CTR, BECKMAN RES INT, DEPT NEUROL, DUARTE, CA 91010 USA. CITY HOPE NATL MED CTR, BECKMAN RES INT, DEPT PEDIAT, DUARTE, CA 91010 USA. NIAID, AIDS PROGRAM, ROCKVILLE, MD 20852 USA. LOMA LINDA UNIV, SCH MED, DEPT BIOCHEM, LOMA LINDA, CA 92350 USA. RP ROSSI, JJ (reprint author), CITY HOPE NATL MED CTR, BECKMAN RES INT, DEPT MOLEC GENET, DUARTE, CA 91010 USA. NR 27 TC 1 Z9 1 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 184 EP 200 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900018 ER PT J AU FRIDLAND, A JOHNSON, MA COONEY, DA AHLUWALIA, G MARQUEZ, VE DRISCOLL, JS JOHNS, DG AF FRIDLAND, A JOHNSON, MA COONEY, DA AHLUWALIA, G MARQUEZ, VE DRISCOLL, JS JOHNS, DG TI METABOLISM IN HUMAN-LEUKOCYTES OF ANTI-HIV DIDEOXYPURINE NUCLEOSIDES SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID HUMAN LYMPHOID-CELLS; CELLULAR PHARMACOLOGY; INFECTIVITY; 2',3'-DIDEOXYADENOSINE; 2',3'-DIDEOXYINOSINE; IDENTIFICATION; INHIBITION; ACTIVATION C1 NCI, DIV CANC TREATMENT, DEV THERAPEUT PROGRAM, BETHESDA, MD 20892 USA. RP FRIDLAND, A (reprint author), ST JUDE CHILDRENS RES HOSP, DEPT BIOCHEM & CLIN PHARMACOL, MEMPHIS, TN 38101 USA. RI Johnson, Mark A/P-5208-2014 OI Johnson, Mark A/0000-0001-6721-2428 NR 12 TC 0 Z9 0 U1 1 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 205 EP 216 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900020 ER PT J AU GORDON, MR TRUCKENMILLER, ME RECKER, DP DICKERSON, DR KUTA, E KULAGA, H KINDT, TJ AF GORDON, MR TRUCKENMILLER, ME RECKER, DP DICKERSON, DR KUTA, E KULAGA, H KINDT, TJ TI EVIDENCE FOR HIV-1 INFECTION IN RABBITS - A POSSIBLE MODEL FOR AIDS SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID ACQUIRED IMMUNODEFICIENCY SYNDROME; T-CELL; ANIMAL-MODEL; VIRUS; CHIMPANZEES C1 NIAID, IMMUNOGENET LAB, BLDG 4, ROOM 213, BETHESDA, MD 20892 USA. ST ELIZABETH HOSP, NIMH, NEUROPSYCHIAT BRANCH, WASHINGTON, DC 20032 USA. NR 25 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 270 EP 280 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900026 ER PT J AU YARCHOAN, R PLUDA, JM PERNO, CF MITSUYA, H THOMAS, RV WYVILL, KM BRODER, S AF YARCHOAN, R PLUDA, JM PERNO, CF MITSUYA, H THOMAS, RV WYVILL, KM BRODER, S TI INITIAL CLINICAL-EXPERIENCE WITH DIDEOXYNUCLEOSIDES AS SINGLE AGENTS AND IN COMBINATION THERAPY SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; AIDS-RELATED COMPLEX; COLONY-STIMULATING FACTOR; PLACEBO-CONTROLLED TRIAL; ZIDOVUDINE AZT; REVERSE-TRANSCRIPTASE; AZIDOTHYMIDINE AZT; HIV INFECTION; HTLV-III/LAV; DOUBLE-BLIND RP YARCHOAN, R (reprint author), NCI, CLIN ONCOL PROGRAM, BETHESDA, MD 20892 USA. RI perno, carlo federico/O-1544-2016 NR 60 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 328 EP 343 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900032 ER PT J AU WEINSTEIN, JN BUNOW, B WEISLOW, OS SCHINAZI, RF WAHL, SM WAHL, LM SZEBENI, J AF WEINSTEIN, JN BUNOW, B WEISLOW, OS SCHINAZI, RF WAHL, SM WAHL, LM SZEBENI, J TI SYNERGISTIC DRUG-COMBINATIONS IN AIDS THERAPY - DIPYRIDAMOLE/3'-AZIDO-3'-DEOXYTHYMIDINE IN PARTICULAR AND PRINCIPLES OF ANALYSIS IN GENERAL SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID IMMUNODEFICIENCY-VIRUS TYPE-1; COLON CANCER-CELLS; CENTRIFUGAL ELUTRIATION CCE; MONOCYTE-ENRICHED FRACTIONS; CYTO-TOXICITY; ANIMAL-CELLS; PROGENITOR CELLS; NONFACILITATED DIFFUSION; NUCLEOSIDE TRANSPORTERS; FLUOROURACIL METABOLISM C1 NCI, FREDERICK CANC RES FACIL, FREDERICK, MD 21701 USA. CIVILIZED SOFTWARE INC, BETHESDA, MD 20814 USA. PROGRAM RESOURCES INC, FREDERICK, MD USA. VET AFFAIRS MED CTR, DEPT PEDIAT, ATLANTA, GA 30033 USA. EMORY UNIV, SCH MED, ATLANTA, GA 30033 USA. NIDR, IMMUNOL LAB, BETHESDA, MD 20892 USA. RP WEINSTEIN, JN (reprint author), NCI, BETHESDA, MD 20892 USA. RI Schinazi, Raymond/B-6777-2017 NR 68 TC 0 Z9 0 U1 1 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 367 EP 384 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900036 ER PT J AU FRANK, KB CONNELL, EV HOLMAN, MJ HURYN, DM SLUBOSKI, BC TAM, SY TODARO, LJ WEIGELE, M RICHMAN, DD MITSUYA, H BRODER, S SIM, IS AF FRANK, KB CONNELL, EV HOLMAN, MJ HURYN, DM SLUBOSKI, BC TAM, SY TODARO, LJ WEIGELE, M RICHMAN, DD MITSUYA, H BRODER, S SIM, IS TI ANABOLISM AND MECHANISM OF ACTION OF RO24-5098, AN ISOMER OF 2',3'-DIDEOXYADENOSINE (DDA) WITH ANTI-HIV ACTIVITY SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID HUMAN LYMPHOID-CELLS; CELLULAR PHARMACOLOGY; ZIDOVUDINE AZT; 2',3'-DIDEOXYCYTIDINE; 2',3'-DIDEOXYINOSINE; INFECTIVITY; INHIBITION; METABOLISM; TOXICITY; INVITRO C1 UNIV CALIF SAN DIEGO, SAN DIEGO, CA 92103 USA. NCI, BETHESDA, MD 20892 USA. RP FRANK, KB (reprint author), HOFFMANN LA ROCHE INC, ROCHE RES CTR, NUTLEY, NJ 07110 USA. NR 15 TC 1 Z9 1 U1 0 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 408 EP 414 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900039 ER PT J AU BUNOW, B WEINSTEIN, JN AF BUNOW, B WEINSTEIN, JN TI COMBO - A NEW APPROACH TO THE ANALYSIS OF DRUG-COMBINATIONS INVITRO SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article C1 NCI, BETHESDA, MD 20892 USA. RP BUNOW, B (reprint author), CIVILIZED SOFTWARE INC, BETHESDA, MD 20814 USA. NR 6 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 490 EP 494 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900052 ER PT J AU CUSHMAN, M OH, YI COPELAND, TD SNYDER, SW OROSZLAN, S AF CUSHMAN, M OH, YI COPELAND, TD SNYDER, SW OROSZLAN, S TI AN APPROACH TO THE SYNTHESIS OF HIV PROTEASE INHIBITORS - STEREOCHEMICALLY PURE PEPTIDE SUBSTRATE-ANALOGS CONTAINING [PHE-PSI-CH2N-PRO] LINKAGES SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article C1 NCI, FREDERICK CANC RES FACIL, MOLEC VIROL & CARCINOGENESIS, BRI BAS RES PROGRAM, FREDERICK, MD 21701 USA. RP CUSHMAN, M (reprint author), PURDUE UNIV, DEPT MED CHEM & PHARMACOGNOSY, W LAFAYETTE, IN 47907 USA. NR 8 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 503 EP 507 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900055 ER PT J AU GREENBERG, ML ALLAUDEEN, HS HERSHFIELD, MS AF GREENBERG, ML ALLAUDEEN, HS HERSHFIELD, MS TI METABOLISM, TOXICITY, AND ANTI-HIV ACTIVITY OF 2'-DEOXY-3'-THIA-CYTIDINE (BCH-189) IN T-CELL AND B-CELL LINES SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article C1 NIH, DIV AIDS, ROCKVILLE, MD USA. RP GREENBERG, ML (reprint author), DUKE UNIV, MED CTR, DEPT CHEM, DURHAM, NC 27710 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 517 EP 518 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900059 ER PT J AU HARTMAN, NR JOHNS, DG MITSUYA, H AF HARTMAN, NR JOHNS, DG MITSUYA, H TI PHARMACOKINETIC STUDY OF DEXTRAN SULFATE IN RATS SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article RP HARTMAN, NR (reprint author), NCI, BETHESDA, MD 20892 USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 523 EP 525 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900061 ER PT J AU HURYN, DM SLUBOSKI, BC TAM, SY TODARO, LJ WEIGELE, M SIM, IS FRANK, KB RICHMAN, DD MITSUYA, H BRODER, S AF HURYN, DM SLUBOSKI, BC TAM, SY TODARO, LJ WEIGELE, M SIM, IS FRANK, KB RICHMAN, DD MITSUYA, H BRODER, S TI SYNTHESIS AND ANTI-HIV ACTIVITY OF A NOVEL SERIES OF ISOMERIC DIDEOXYNUCLEOSIDES SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; NUCLEOSIDE C1 UNIV CALIF SAN DIEGO, VET ADM MED CTR, LA JOLLA, CA 92093 USA. NCI, CLIN ONCOL PROGRAM, BETHESDA, MD 20892 USA. RP HURYN, DM (reprint author), HOFFMANN LA ROCHE INC, ROCHE RES CTR, NUTLEY, NJ 07110 USA. NR 6 TC 0 Z9 0 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 530 EP 534 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900064 ER PT J AU NASR, M LITTERST, C MCGOWAN, J AF NASR, M LITTERST, C MCGOWAN, J TI STRUCTURE-ACTIVITY CORRELATIONS OF PYRIMIDINE AND PURINE DIDEOXYNUCLEOSIDES AS POTENTIAL ANTI-HIV DRUGS SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID AGENTS RP NASR, M (reprint author), NIAID, DIV AIDS, BETHESDA, MD 20892 USA. NR 4 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 579 EP 587 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900078 ER PT J AU SAGER, PR CRADOCK, JC LITTERST, CL MARTIN, LN SOIKE, KF MURPHEYCORB, M MARX, PA TSAI, CC FRIDLAND, A BODNER, A RESNICK, L SCHINAZI, RF AF SAGER, PR CRADOCK, JC LITTERST, CL MARTIN, LN SOIKE, KF MURPHEYCORB, M MARX, PA TSAI, CC FRIDLAND, A BODNER, A RESNICK, L SCHINAZI, RF TI INVITRO TESTING OF THERAPEUTICS AGAINST SIV AND HIV - A COMPARISON SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID SIMIAN IMMUNODEFICIENCY VIRUS; REVERSE-TRANSCRIPTASE; SYNCYTIUM FORMATION; INHIBITION; 3'-AZIDO-3'-DEOXYTHYMIDINE; INFECTIVITY; 2',3'-DIDEOXYNUCLEOSIDES; CASTANOSPERMINE; GLUCOSIDASE C1 TULANE UNIV, DELTA REG PRIMATE RES CTR, COVINGTON, LA 70433 USA. UNIV CALIF DAVIS, CALIF PRIMATE RES CTR, DAVIS, CA 95616 USA. UNIV WASHINGTON, REG PRIMATE RES CTR, MEDICAL LAKE, WA 99022 USA. ST JUDE CHILDRENS RES HOSP, MEMPHIS, TN 38101 USA. BIOTECH RES LABS INC, ROCKVILLE, MD 20850 USA. MT SINAI MED CTR, MIAMI BEACH, FL 33140 USA. EMORY UNIV, SCH MED, VET ADM MED CTR, ATLANTA, GA 30032 USA. RP SAGER, PR (reprint author), NIAID, DIV AIDS, DEV THERAPEUT BRANCH, BETHESDA, MD 20892 USA. RI Schinazi, Raymond/B-6777-2017 NR 13 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 599 EP 605 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900083 ER PT J AU SARVER, N HAMPEL, A CANTIN, EM ZAIA, JA CHANG, PS JOHNSTON, MI MCGOWAN, J ROSSI, JJ AF SARVER, N HAMPEL, A CANTIN, EM ZAIA, JA CHANG, PS JOHNSTON, MI MCGOWAN, J ROSSI, JJ TI SELF-CLEAVING RNAS (RIBOZYMES) AS NEW MODALITIES FOR ANTI-HIV THERAPY SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article C1 NO ILLINOIS UNIV, CTR PLANT MOLEC BIOL, DE KALB, IL 60115 USA. CITY HOPE NATL MED CTR, DUARTE, CA 91010 USA. RP SARVER, N (reprint author), NIAID, DIV AIDS, BETHESDA, MD 20892 USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 606 EP 609 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900084 ER PT J AU STERN, S MUUL, L MILMAN, G AF STERN, S MUUL, L MILMAN, G TI NIH NIAID-WHO AIDS RESEARCH AND REFERENCE REAGENT PROGRAM SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article RP STERN, S (reprint author), NIAID, PATHOGENESIS BRANCH, AIDS PROGRAM, 649A LOFSTRAND LANE, ROCKVILLE, MD 20850 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 610 EP 610 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900085 ER PT J AU SZEBENI, J WAHL, SM SCHINAZI, RF POPOVIC, M GARTNER, S WAHL, LM WEISLOW, OS BETAGERI, G FINE, RL DAHLBERG, JE HUNTER, E WEINSTEIN, JN AF SZEBENI, J WAHL, SM SCHINAZI, RF POPOVIC, M GARTNER, S WAHL, LM WEISLOW, OS BETAGERI, G FINE, RL DAHLBERG, JE HUNTER, E WEINSTEIN, JN TI DIPYRIDAMOLE POTENTIATES THE ACTIVITY OF ZIDOVUDINE AND OTHER DIDEOXYNUCLEOSIDES AGAINST HIV-1 IN CULTURED-CELLS SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID MONOCYTE MACROPHAGES; 3'-AZIDO-3'-DEOXYTHYMIDINE C1 NCI, TUMOR CELL BIOL LAB, BETHESDA, MD 20892 USA. NCI, MED BRANCH, BETHESDA, MD 20892 USA. NIDR, MICROBIOL & IMMUNOL LAB, BETHESDA, MD 20892 USA. NCI, FREDERICK CANC RES FACIL, FREDERICK, MD 21701 USA. EMORY UNIV, SCH MED, VET ADM MED CTR, DECATUR, GA 30033 USA. PAN DATA SYST INC, ROCKVILLE, MD 20850 USA. RP SZEBENI, J (reprint author), NCI, MATH BIOL LAB, BETHESDA, MD 20892 USA. RI Schinazi, Raymond/B-6777-2017 NR 5 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 613 EP 616 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900087 ER PT J AU WILLIAMS, GJ COLBY, CB SCHINAZI, RF SOMMADOSSI, JP CHU, CK JOHNS, DG MITSUYA, H AF WILLIAMS, GJ COLBY, CB SCHINAZI, RF SOMMADOSSI, JP CHU, CK JOHNS, DG MITSUYA, H TI THE CELLULAR-METABOLISM OF AZDU - CORRELATION WITH INVITRO ANTI-HIV AND CYTOTOXIC ACTIVITIES SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article C1 VET ADM MED CTR, ATLANTA, GA USA. EMORY UNIV, SCH MED, ATLANTA, GA 30322 USA. UNIV ALABAMA, BIRMINGHAM, AL 35294 USA. UNIV GEORGIA, ATHENS, GA 30602 USA. NCI, BETHESDA, MD 20892 USA. RP WILLIAMS, GJ (reprint author), TRITON BIOSCI INC, ALAMEDA, CA 94501 USA. RI Schinazi, Raymond/B-6777-2017 NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 26 PY 1990 VL 616 BP 620 EP 623 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA FM709 UT WOS:A1990FM70900089 ER PT J AU BOLOS, AM DEAN, M LUCASDERSE, S RAMSBURG, M BROWN, GL GOLDMAN, D AF BOLOS, AM DEAN, M LUCASDERSE, S RAMSBURG, M BROWN, GL GOLDMAN, D TI POPULATION AND PEDIGREE STUDIES REVEAL A LACK OF ASSOCIATION BETWEEN THE DOPAMINE-D2 RECEPTOR GENE AND ALCOHOLISM SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article C1 NIAAA,CLIN STUDIES LAB,GENET STUDIES LAB,9000 ROCKVILLE PIKE,BLDG 10,ROOM 3C-102,ROCKVILLE,MD 20852. PROGRAM RESOURCES INC,FREDERICK,MD. NIAAA,CLIN STUDIES LAB,FAMILY STUDIES UNIT,ROCKVILLE,MD 20852. RI Dean, Michael/G-8172-2012; Goldman, David/F-9772-2010 OI Dean, Michael/0000-0003-2234-0631; Goldman, David/0000-0002-1724-5405 FU NCI NIH HHS [N01-CO-74102] NR 28 TC 198 Z9 201 U1 2 U2 5 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 26 PY 1990 VL 264 IS 24 BP 3156 EP 3160 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA EN614 UT WOS:A1990EN61400035 PM 1979357 ER PT J AU HALL, WH AF HALL, WH TI INTRAVENOUS IMMUNOGLOBULIN - PREVENTION AND TREATMENT OF DISEASE SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP HALL, WH (reprint author), NIH,OFF MED APPLICAT RES,BLDG 1,ROOM 260,BETHESDA,MD 20892, USA. NR 0 TC 66 Z9 67 U1 1 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 26 PY 1990 VL 264 IS 24 BP 3189 EP 3193 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA EN614 UT WOS:A1990EN61400041 ER PT J AU BLUMENTHAL, SJ AF BLUMENTHAL, SJ TI YOUTH SUICIDE - THE PHYSICIANS ROLE IN SUICIDE-PREVENTION SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material C1 GEORGETOWN UNIV,SCH MED,DEPT PSYCHIAT,WASHINGTON,DC 20057. RP BLUMENTHAL, SJ (reprint author), NIMH,BEHAV MED RES PROGRAM,5600 FISHERS LN,ROOM 11C-06,ROCKVILLE,MD 20857, USA. NR 16 TC 16 Z9 17 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 26 PY 1990 VL 264 IS 24 BP 3194 EP 3195 DI 10.1001/jama.264.24.3194 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA EN614 UT WOS:A1990EN61400042 PM 2255029 ER PT J AU HAVUNJIAN, RH DECOSTA, BR RICE, KC SKOLNICK, P AF HAVUNJIAN, RH DECOSTA, BR RICE, KC SKOLNICK, P TI CHARACTERIZATION OF BENZODIAZEPINE RECEPTORS WITH A FLUORESCENCE-QUENCHING LIGAND SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID BETA-ADRENERGIC RECEPTORS; NON-SPECIFIC BINDING; ALPRENOLOL-NBD; SITES; VISUALIZATION; COMPLEX; ANALOG; PROBES AB A conjugate of the high affinity benzodiazepine receptor ligand Ro 15-1788 and the fluorescent 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) moiety was synthesized. This novel compound (BD 623) exhibited excitation and emission maxima at 486 and 542 nm, respectively, and possessed fluorescent properties that are dependent upon the polarity of its environment. BD 623 bound reversibly to benzodiazepine receptors in the central nervous system with an apparent affinity (K(i) 5.7 nM) comparable to the parent imidazobenzodiazepine (K(d) 2.8 nM). Addition of BD 623 to a suspension of brain membranes resulted in a time-dependent quenching of its fluorescence. Fluorescence quenching of this compound was readily reversed by specific benzodiazepine receptor ligands but not by a variety of other substances. Moreover, inactivation of benzodiazepine receptors by photoaffinity labeling with Ro 15-4513 resulted in a reduction in the fluorescence quenching of BD 623 consistent with the reduction in density of benzodiazepine receptors measured using a radioreceptor assay. Monitoring of fluorescence/dequenching of BD 623 in real time permitted a quantitative characterization of the ligand-receptor interaction, with both the K(d) of BD 623 (13.9 nM) and K(i) of Ro 15-1788 (5.7 nM) comparable with the estimates obtained using radioreceptor techniques. These results indicate that application of fluorescence quenching techniques with BD 623 could prove a useful adjunct for the study of benzodiazepine receptors. BD 623 may serve as a prototype for the development of other fluorescent ligands to study ligand-receptor interactions. C1 NIDDK,NEUROSCI LAB,BLDG 8,ROOM 111,BETHESDA,MD 20892. NIDDK,MED CHEM LAB,BETHESDA,MD 20892. NR 24 TC 15 Z9 15 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 25 PY 1990 VL 265 IS 36 BP 22181 EP 22186 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EP556 UT WOS:A1990EP55600023 PM 2176206 ER PT J AU KASHIWAGI, K TANEJA, SK LIU, TY TABOR, CW TABOR, H AF KASHIWAGI, K TANEJA, SK LIU, TY TABOR, CW TABOR, H TI SPERMIDINE BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE - BIOSYNTHESIS AND PROCESSING OF A PROENZYME FORM OF S-ADENOSYLMETHIONINE DECARBOXYLASE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID DOUBLE-STRANDED-RNA; ESCHERICHIA-COLI; HISTIDINE-DECARBOXYLASE; SEQUENCE-ANALYSIS; ALPHA-CHAIN; RAT-LIVER; PYRUVATE; YEAST; LACTOBACILLUS-30A; PLASMID AB We have cloned and sequenced the Saccharomyces cerevisiae gene for S-adenosylmethionine decarboxylase. This enzyme contains covalently bound pyruvate which is essential for enzymatic activity. We have shown that this enzyme is synthesized as a M(r) 46,000 proenzyme which is then cleaved post-translationally to form two polypeptide chains: a beta-subunit (M(r) 10,000) from the amino-terminal portion and an alpha-subunit (M(r) 36,000) from the carboxyl-terminal portion. The protein was overexpressed in Escherichia coli and purified to homogeneity. The purified enzyme contains both the alpha and beta-subunits. About half of the alpha-subunits have pyruvate blocking the amino-terminal end; the remaining-alpha-subunits have alanine in this position. From a comparison of the amino acid sequence deduced from the nucleotide sequence with the amino acid sequence of the amino-terminal portion of each subunit (determined by Edman degradation), we have identified the cleavage site of the proenzyme as the peptide bond between glutamic acid 87 and serine 88. The pyruvate moiety, which is essential for activity, is generated from serine 88 during the cleavage. The amino acid sequence of the yeast enzyme has essentially no homology with S-adenosylmethionine decarboxylase of E. coli (Tabor, C. W., and Tabor, H. (1987) J. Biol. Chem. 262, 16037-16040) and only a moderate degree of homology with the human and rat enzymes (Pajunen, A., Crozat, A., Janne, O. A., Ihalainen, R., Laitinen, P. H., Stanley, B., Madhubala, R., and Pegg, A. E. (1988) J. Biol. Chem. 263, 17040-17049); all of these enzymes are pyruvoyl-containing proteins. Despite this limited overall homology the cleavage site of the yeast proenzyme is identical to the cleavage sites in the human and rat proenzymes, and seven of the eight amino acids adjacent to the cleavage site are identical in the three eukaryote enzymes. C1 NIDDK,BIOCHEM PHARMACOL LAB,PHARMACOL SECT,BLDG 8,RM 223,BETHESDA,MD 20892. US FDA,CTR BIOL EVALUAT & RES,DIV BIOCHEM & BIOPHYS,BETHESDA,MD 20892. NR 48 TC 60 Z9 63 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 25 PY 1990 VL 265 IS 36 BP 22321 EP 22328 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EP556 UT WOS:A1990EP55600044 PM 2266128 ER PT J AU ALPERT, CA CHASSY, BM AF ALPERT, CA CHASSY, BM TI MOLECULAR-CLONING AND DNA-SEQUENCE OF LACE, THE GENE ENCODING THE LACTOSE-SPECIFIC ENZYME-II OF THE PHOSPHOTRANSFERASE SYSTEM OF LACTOBACILLUS-CASEI - EVIDENCE THAT A CYSTEINE RESIDUE IS ESSENTIAL FOR SUGAR PHOSPHORYLATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID AMINO-ACID-SEQUENCE; PHOSPHOGALACTOSIDE GALACTOHYDROLASE GENE; BETA-GALACTOSIDASE GENE; ESCHERICHIA-COLI K-12; STAPHYLOCOCCUS-AUREUS; NUCLEOTIDE-SEQUENCE; BACTERIAL PHOSPHOENOLPYRUVATE; CARRIER PROTEIN; FACTOR-IIILAC; MANNITOL AB The gene coding for the lactose-specific Enzyme II of the Lactobacillus casei phosphoenolpyruvate-dependent phosphotransferase system, lacE, has been isolated by molecular cloning and expressed in Escherichia coli. The DNA sequence of the lacE gene and the deduced amino acid sequence are presented. The putative translation product comprises a hydrophobic protein of 577 amino acids with a calculated molecular mass of 62,350 Da. The deduced polypeptide has a high degree of sequence similarity with the corresponding lactose-specific enzymes II of Staphylococcus aureus and Lactococcus lactis. The sequence surrounding cysteine 483 was strongly conserved in the three proteins. The identity of the lacE product as the Enzyme II(lac)L.casei was demonstrated by in vitro lactose phosphorylation assays using the protein expressed in E. coli. Single replacement of each of the histidine and cysteine residues by site-directed mutagenesis pointed to cysteine 483 as an amino acid residue essential for the phosphoryl group transfer reaction. C1 NIDR,MICROBIAL ECOL LAB,BETHESDA,MD 20892. NR 60 TC 41 Z9 43 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 25 PY 1990 VL 265 IS 36 BP 22561 EP 22568 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EP556 UT WOS:A1990EP55600078 PM 2125053 ER PT J AU MILLS, FC BROOKER, JS CAMERINIOTERO, RD AF MILLS, FC BROOKER, JS CAMERINIOTERO, RD TI SEQUENCES OF HUMAN-IMMUNOGLOBULIN SWITCH REGIONS - IMPLICATIONS FOR RECOMBINATION AND TRANSCRIPTION SO NUCLEIC ACIDS RESEARCH LA English DT Article ID HEAVY-CHAIN GENES; SACCHAROMYCES-CEREVISIAE; ALPHA-INTERFERON; GAMMA-INTERFERON; BINDING PROTEIN; EPSILON-GENES; B-CELLS; DNA; EXPRESSION; HOMOLOGY AB We have sequenced the entire human S-mu and S-gamma-4 immunoglobulin heavy chain class switch regions, and have also completed the sequence of human S-epsilon. S-mu is composed predominantly of GAGCT and GGGCT pentameric repeats, with these units also being found in S-epsilon at a much lower density. S-mu-S-gamma-4 matches are infrequent, but S-gamma-4 contains a cluster of repeated sequences similar to units in mouse-gamma switch sites and unrelated to the S-mu repeats, suggesting that S-mu-S-gamma homology is not important in mu-gamma switching. We examined our epsilon and gamma-4 sequences for features that could regulate production of 'sterile' transcripts preceding switch recombination. There is an Evolutionarily Conserved Sequence (ECS) upstream from the human and mouse S-epsilon regions that overlaps and extends 5' to the start sites of human and mouse epsilon sterile transcripts. Similarly, and ECS upstream from S-gamma-4 is homologous to a mouse sequence that overlaps and extends 5' to the start sites for mouse gamma-2b sterile transcripts. The epsilon and gamma-4 conserved segments contain potential interferon Stimulable Response Elements (ISRE's) that are identical between human epsilon and gamma-4. C1 NIDDK,GENET & BIOCHEM BRANCH,BLDG 10,ROOM 9D15,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 59 TC 112 Z9 112 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 25 PY 1990 VL 18 IS 24 BP 7305 EP 7316 DI 10.1093/nar/18.24.7305 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ479 UT WOS:A1990EQ47900013 PM 2124350 ER PT J AU PURUCKER, M BODINE, D LIN, H MCDONAGH, K NIENHUIS, AW AF PURUCKER, M BODINE, D LIN, H MCDONAGH, K NIENHUIS, AW TI STRUCTURE AND FUNCTION OF THE ENHANCER-3' TO THE HUMAN A-GAMMA GLOBIN GENE SO NUCLEIC ACIDS RESEARCH LA English DT Article ID ERYTHROID-SPECIFIC PROTEIN; GAMMA-GLOBIN; TRANSCRIPTION FACTOR; RNA-POLYMERASE; ACCURATE TRANSCRIPTION; NUCLEAR PROTEINS; BINDING PROTEINS; MAMMALIAN-CELLS; TRANSGENIC MICE; BOX REGION AB An enhancer is located immediately 3' to the A-gamma globin gene. We have used DNase I footprinting to map the sites of interaction of nuclear proteins with the DNA sequences of this enhancer. Eight footprints were discovered, distributed over 600 base pairs fo DNA. Three of these contain a consensus binding site for th erythroid specific factor GATA-l. Each of these GATA-1 sites had an enhancer activity when inserted into a reporter plasmid and tested in human erythroleukemia cells. Other footprints within the enhancer contained consensus binding sequences for the ubiquitous, positive regulatory proteins AP2 and CBP-1. An Sp1-like recognition sequence was also identified. Synthetic oligonucleotides encompassing two fo the footprints generated a slowly migrating complex in gel mobility shift assays. The same complex forms on a fragment of the human gamma globin gene promoter extending from -260 to -200. The DNasel footprint of this protein that interacts with the chicken beta globin promoter and enhancer, termed the stage selector element. We propose that this complex of proteins may be involved int he human gamma globin promoter-enhancer interaction. C1 UNIV CALIF LOS ANGELES,HARBOR MED CTR,INST RES & EDUC,DIV MED GENET,TORRANCE,CA 90502. RP PURUCKER, M (reprint author), NHLBI,CLIN HEMATOL BRANCH,9000 ROCKVILLE PIKE,BLDG 10,ROOM 7C-103,BETHESDA,MD 20892, USA. NR 64 TC 50 Z9 50 U1 2 U2 3 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 25 PY 1990 VL 18 IS 24 BP 7407 EP 7415 DI 10.1093/nar/18.24.7407 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ479 UT WOS:A1990EQ47900025 PM 2259631 ER PT J AU SAXENA, JK HAYS, JB ACKERMAN, EJ AF SAXENA, JK HAYS, JB ACKERMAN, EJ TI EXCISION REPAIR OF UV-DAMAGED PLASMID DNA IN XENOPUS OOCYTES IS MEDIATED BY DNA POLYMERASE-ALPHA (AND OR POLYMERASE-DELTA) SO NUCLEIC ACIDS RESEARCH LA English DT Article ID PYRIMIDINE DIMERS; ACTIVE GENE; LAEVIS; ALPHA; CELLS; RNA; FIBROBLASTS; EXPRESSION; EGGS AB We studied DNA repair by injecting plasmids containing random pyrimidine dimers into Xenopus oocytes. We demonstrated excision repair by recovering plasmids and analyzing them with T4 UV endonuclease treatment and alkaline agarose gel electrophoresis. The mechanism for excision repair of these plasmids appears to be processive, rather than distributive, since repair occurs in an 'all or none' fashion. At less than 4-5 dimers/plasmid, nearly all repair occurs within 4-6 hours (approximately 10(10) dimers repaired per oocyte): the oocyte, therefore, has abundant repair activity. Specific antibodies and inhibitors were used to determine enzymes involved in repair. We conclude that DNA polymerase-alpha (and/or delta) is required because repair is inhibited by antibodies to human DNA polymerase-alpha, as well as by aphidicolin, an inhibitor of polymerases-alpha (and/or delta). Repair was not inhibited by hydroxyurea, cytosine beta-D-arabinofuranoside, or inhibitors of topoisomerase II (novabiocin). Oocyte repair does not activate semi-conservative DNA replication, nor is protein synthesis required. Photoreactivation cannot account for repair because dimer removal is independent of exogenous light. C1 NIDDK,GENET & BIOCHEM BRANCH,BETHESDA,MD 20892. NR 26 TC 20 Z9 20 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 25 PY 1990 VL 18 IS 24 BP 7425 EP 7432 DI 10.1093/nar/18.24.7425 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ479 UT WOS:A1990EQ47900027 PM 2175436 ER PT J AU POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR AF POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR TI DINUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN THROMBOSPONDIN GENE (THBS1) SO NUCLEIC ACIDS RESEARCH LA English DT Note RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 4 TC 6 Z9 6 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 25 PY 1990 VL 18 IS 24 BP 7467 EP 7467 DI 10.1093/nar/18.24.7467 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ479 UT WOS:A1990EQ47900050 PM 2259648 ER PT J AU POLYMEROPOULOS, MH RATH, DS XIAO, H MERRIL, CR AF POLYMEROPOULOS, MH RATH, DS XIAO, H MERRIL, CR TI TRINUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN PANCREATIC PHOSPHOLIPASE-A-2 GENE (PLA2) SO NUCLEIC ACIDS RESEARCH LA English DT Note RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 4 TC 83 Z9 83 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 25 PY 1990 VL 18 IS 24 BP 7468 EP 7468 DI 10.1093/nar/18.24.7468-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ479 UT WOS:A1990EQ47900053 PM 2259649 ER PT J AU POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR AF POLYMEROPOULOS, MH XIAO, H RATH, DS MERRIL, CR TI DINUCLEOTIDE REPEAT POLYMORPHISM AT THE INT-2 PROTOONCOGENE LOCUS (INT2) SO NUCLEIC ACIDS RESEARCH LA English DT Note RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 3 TC 83 Z9 83 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 25 PY 1990 VL 18 IS 24 BP 7468 EP 7468 DI 10.1093/nar/18.24.7468-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EQ479 UT WOS:A1990EQ47900052 PM 2259649 ER PT J AU JOSEPH, JA KOWATCH, MA MAKI, T ROTH, GS AF JOSEPH, JA KOWATCH, MA MAKI, T ROTH, GS TI SELECTIVE CROSS-ACTIVATION INHIBITION OF 2ND MESSENGER SYSTEMS AND THE REDUCTION OF AGE-RELATED DEFICITS IN THE MUSCARINIC CONTROL OF DOPAMINE RELEASE FROM PERIFUSED RAT STRIATA SO BRAIN RESEARCH LA English DT Article DE AGING; DOPAMINE; STRIATUM; MUSCARINIC; ARACHIDONIC ACID; 2ND MESSENGER ID NUCLEUS BASALIS MAGNOCELLULARIS; CHOLINERGIC DRUG-COMBINATIONS; RABBIT CAUDATE-NUCLEUS; MOUSE-BRAIN MEMBRANES; MEMORY DEFICITS; PHOSPHOINOSITIDE HYDROLYSIS; DOPAMINE RELEASE; RECEPTOR ACTIVATION; ARACHIDONIC-ACID; EFFECTOR SYSTEMS AB Possible alterations in muscarinic cholinergic (mACh) signal transduction in senescence were studied in rat neostriata. Acetylcholine (ACh) activation of striatal muscarinic heteroreceptors by carbachol or oxotremorine enhances K+ -evoked release to dopamine from perifused striata of 6- but not 24-month-old rats. Present experiments determined the effects of simultaneous activation or activation/inhibition of more than one second messenger on K+-evoked release of DA from perifused striatal slices from these age groups. Combinations of carbachol (500-mu-m), which stimulates inositol-1,4,5-biphosphate (IP3) production and inhibits cyclic AMP production, with oxotremorine (500-mu-m), which inhibits cyclic AMP production, in the presence of 30 mM KCl (in a modified Krebs-Ringer medium) reduced the age-related reduction in mAChR enhancement of DA release (analyzed by HPLC coupled to electrochemical detection; 5 min fractions were collected on ice in perchloric acid; flow rate 120-mu-l/min). Combinations of these agonists with the putative second messenger arachidonic acid (10-mu-M), also enhanced K+-evoked release of DA in the striatal tissue from the 24-month group. IP3 activation was lower in the striatal tissue from old animals than those from young under all conditions, but cross-activation/inhibition actually may have lowered the IP3 threshold necessary for enhanced DA release to occur. In a subsequent experiment, pre-loading striatal tissue from young animals with either carbachol or oxotremorine under basal release conditions reduced the responding when the basal release medium was switched to one containing 30 mM KCl and combinations of the antagonist. Results are discussed in terms of the possible locust of the age-related deficits in mAChR responsivity, and the necessity of utilizing 'cross-talk' among various second messenger systems to offset these decrements. The findings suggest possible new directions for treatments for age-related alterations in cholinergic systems. RP JOSEPH, JA (reprint author), NIA,FRANCIS SCOTT KEY MED CTR,GERONTOL RES CTR,BALTIMORE,MD 21224, USA. NR 66 TC 40 Z9 40 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD DEC 24 PY 1990 VL 537 IS 1-2 BP 40 EP 48 DI 10.1016/0006-8993(90)90337-B PG 9 WC Neurosciences SC Neurosciences & Neurology GA EP961 UT WOS:A1990EP96100005 PM 1964841 ER PT J AU SZALLASI, A SZALLASI, Z BLUMBERG, PM AF SZALLASI, A SZALLASI, Z BLUMBERG, PM TI PERMANENT EFFECTS OF NEONATALLY ADMINISTERED RESINIFERATOXIN IN THE RAT SO BRAIN RESEARCH LA English DT Article DE RESINIFERATOXIN; CAPSAICIN-SENSITIVE NEURON; SENSOROTOXICITY; NEWBORN RAT ID PRIMARY SENSORY NEURONS; GENE-RELATED PEPTIDE; SUBSTANCE-P; SELECTIVE DEGENERATION; CAPSAICIN TREATMENT; ADULT-RAT; SOMATOSTATIN; MOUSE; AXONS; TIME AB We have previously demonstrated that resiniferatoxin functions in adult rats as an ultrapotent analog of capsaicin. In adults, capsaicin excites and then desensitizes a specific population of sensory neurons; when administered to neonates capsaicin causes degeneration of these neurons. We report here that treatment of newborn rats with resiniferatoxin caused a substantial (47%) loss of dorsal root ganglia neurons in adults and an almost complete loss of calcitonin gene related peptide-like immunoreactivity in both dorsal root ganglia and gasserian ganglia. The animals were unresponsive to noxious chemical stimuli and showed marked diminution (88%) of their neurogenic inflammatory response. Resiniferatoxin was at least 2 orders of magnitude more potent than capsaicin for inducing neurodegeneration in the neonates. Specific resiniferatoxin binding, though to represent capsaicin receptors, decreased 80-90% in membranes from dorsal root ganglia and 50-70% in membranes from gasserian ganglia of adult rats treated neonatally with resiniferatoxin. The affinity for the residual binding decreased. We speculate that subpopulations of sensory neurons differ in susceptibility to neonatal resiniferatoxin treatment. Resiniferatoxin promises to be a useful probe to explore mechanisms of sensorotoxin-induced degeneration for subpopulations of capsaicin-sensitive sensory neurons. C1 NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892. NR 33 TC 12 Z9 12 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD DEC 24 PY 1990 VL 537 IS 1-2 BP 182 EP 186 DI 10.1016/0006-8993(90)90356-G PG 5 WC Neurosciences SC Neurosciences & Neurology GA EP961 UT WOS:A1990EP96100024 PM 2085772 ER PT J AU BAGDY, G CALOGERO, AE SZEMEREDI, K GOMEZ, MT MURPHY, DL CHROUSOS, GP GOLD, PW AF BAGDY, G CALOGERO, AE SZEMEREDI, K GOMEZ, MT MURPHY, DL CHROUSOS, GP GOLD, PW TI BETA-ENDORPHIN RESPONSES TO DIFFERENT SEROTONIN AGONISTS - INVOLVEMENT OF CORTICOTROPIN-RELEASING HORMONE, VASOPRESSIN AND DIRECT PITUITARY ACTION SO BRAIN RESEARCH LA English DT Article DE SEROTONIN; SEROTONIN-1A RECEPTOR; SEROTONIN-1C RECEPTOR; SEROTONIN-2 RECEPTOR; CORTICOTROPIN-RELEASING HORMONE; VASOPRESSIN; BETA-ENDORPHIN; HYPOTHALAMUS; PITUITARY ID CONSCIOUS RATS; ARGININE VASOPRESSIN; ACTH RELEASE; SECRETION; RECEPTORS; ADRENOCORTICOTROPIN; STIMULATION; ACTIVATION; MECHANISM; SYSTEM AB Activation of serotonergic neurotransmission has been shown to increase plasma beta-endorphin-like immunoreactivity (beta-End-LI). To study the mechanism(s) of this action, we measured the effects of 3 potent serotonin (5-HT) agonists with different structures and 5-HT receptor binding profiles in conscious unrestrained Sprague-Dawley rats in vivo and in dispersed anterior pituicytes in vitro. The 5-HT1A agonist, 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), the 5-HT1c agonist, m-chlorophenylpiperazine (m-CPP), and the 5-HT2 agonist, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI), all markedly increased beta-End-LI in plasma in vivo. All 3 responses were blocked by dexamethasone pretreatment. Pituitary stalk transection (PST), as well as pretreatment with rabbit serum hyperimmune against rat corticotropin-releasing hormone (CRH, TS-6) completely abolished beta-End-LI response to 8-OH-DPAT and attenuated the responses by about 60% to DOI. Responses to m-CPP were markedly attenuated in PST rats, but pretreatment with TS-6 had no significant effect. To examine whether vasopressin (AVP) might be involved in the CRH neutralizing antibody-resistant beta-End-LI responses after m-CPP and DOI, we measured AVP concentrations after each agonist. m-CPP, but DOI or 8-OH-DPAT, significantly elevated circulating AVP levels. As a proof of direct pituitary effect, DOI markedly stimulated beta-End-LI release from the anterior pituitary cell culture preparation in vitro. It was approximately as potent as CRH in the picomolar range. m-CPP was much less effective than DOI, while 8-OH-DPAT did not stimulate beta-End-LI release in vitro. Thus, the present findings suggest that the 5-HT1A agonist 8-OH-DPAT causes plasma beta-End-LI increase in vivo by stimulating hypothalamic CRH secretion. The mechanism of beta-End-LI response to the 5-HT1C agonist m-CPP is a complex phenomenon including mainly AVP-mediated and directed pituitary actions. The 5-HT2 agonist DOI may elicit beta-End-LI responses by CRH-mediated and direct pituitary effects. C1 NHLBI,HYPERTENS & ENDOCRINOL BRANCH,BETHESDA,MD 20892. NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. NIMH,CLIN SCI LAB,BETHESDA,MD 20892. RP BAGDY, G (reprint author), NIMH,CTR CLIN,CLIN NEUROENDOCRINOL BRANCH,10-3S231,BETHESDA,MD 20892, USA. OI Bagdy, Gyorgy/0000-0001-8141-3410 NR 27 TC 40 Z9 41 U1 0 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD DEC 24 PY 1990 VL 537 IS 1-2 BP 227 EP 232 DI 10.1016/0006-8993(90)90362-F PG 6 WC Neurosciences SC Neurosciences & Neurology GA EP961 UT WOS:A1990EP96100030 PM 2150776 ER PT J AU LADENHEIM, EE JENSEN, RT MANTEY, SA MCHUGH, PR MORAN, TH AF LADENHEIM, EE JENSEN, RT MANTEY, SA MCHUGH, PR MORAN, TH TI RECEPTOR HETEROGENEITY FOR BOMBESIN-LIKE PEPTIDES IN THE RAT CENTRAL-NERVOUS-SYSTEM SO BRAIN RESEARCH LA English DT Article DE BOMBESIN; GASTRIN-RELEASING PEPTIDE; NEUROMEDIN-B; AUTORADIOGRAPHY; NUCLEUS-ACCUMBENS; NUCLEUS-TRACTUS-SOLITARII; PANCREAS; CORTEX ID GASTRIN RELEASING PEPTIDE; PANCREATIC ACINAR-CELLS; PORCINE SPINAL-CORD; NEUROMEDIN-B; BINDING-SITES; BRAIN; LOCALIZATION; SYNAPTOSOMES; ANTAGONISTS; ANALOGS AB As an initial characterization of bombesin binding sites in the rat central nervous system, we examined the pharmacological specificity of binding of bombesin (BBS) and several BBS analogs to rat cortex and compared these results to those for rat pancreas. In addition, we used in vitro receptor autoradiography to evaluate binding I-125[Tyr4]bombesin and I-125-Bolton-Hunter neuromedin B (NMB) to several regions of the rat brain. The results of the pharmacological study indicated that the pancreas and cortex had different binding affinities for BBS-like peptides. While cortical binding sites had a high affinity for NMB, pancreatic binding sites had almost no affinity for NMB. Results from the autoradiographic study demonstrated that BBS receptor heterogeneity exists in individual nuclei in the rat brain. Some nuclei have a high affinity for NMB, similar to cortical BBS binding sites, other regions have a low affinity similar to pancreatic BBS binding sites. These results provide evidence that subtypes of BBS receptors are present in different tissues and within discrete regions of the rat central nervous system C1 NIDDKD,DIGEST DIS BRANCH,BETHESDA,MD 20892. RP LADENHEIM, EE (reprint author), JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT & BEHAV SCI,MEYER 4-119,600 N WOLFE ST,BALTIMORE,MD 21205, USA. NR 25 TC 62 Z9 62 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD DEC 24 PY 1990 VL 537 IS 1-2 BP 233 EP 240 DI 10.1016/0006-8993(90)90363-G PG 8 WC Neurosciences SC Neurosciences & Neurology GA EP961 UT WOS:A1990EP96100031 PM 1964836 ER PT J AU MAJEWSKA, MD BELL, JA LONDON, ED AF MAJEWSKA, MD BELL, JA LONDON, ED TI REGULATION OF THE NMDA RECEPTOR BY REDOX PHENOMENA - INHIBITORY ROLE OF ASCORBATE SO BRAIN RESEARCH LA English DT Note DE N-METHYL-D-ASPARTATE RECEPTOR; REDOX; ASCORBIC ACID ID D-ASPARTATE RECEPTORS; N-METHYL-ASPARTATE; RAT-BRAIN; NEURONS; ACID; BINDING; PHENCYCLIDINE; SITES; ANTAGONIST; MEMBRANES AB Redox phenomena seem to modulate activity of the N-methyl-D-aspartate receptor. Some reductants (ascorbate, hydroquinone) inhibit, while others (dithiothreitol, mercaptoethanol, penicillamine) potentiate NMDA receptor function. Ascorbate inhibits binding of [H-3]glutamate and [H-3]thienylcyclohexylpiperidine to the NMDA receptor complex, and impedes NMDA-gated currents in isolated neurons; dithiothreitol-like reductants enhance NMDA-induced currents. The ability of reductants to alter functions of the NMDA receptor is abolished by oxidation. RP MAJEWSKA, MD (reprint author), NIDA,ADDICT RES CTR,NEUROSCI BRANCH,NEUROPHARMACOL LAB,POB 5180,BALTIMORE,MD 21224, USA. NR 30 TC 123 Z9 124 U1 3 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD DEC 24 PY 1990 VL 537 IS 1-2 BP 328 EP 332 DI 10.1016/0006-8993(90)90379-P PG 5 WC Neurosciences SC Neurosciences & Neurology GA EP961 UT WOS:A1990EP96100047 PM 1964838 ER PT J AU WHITE, G LOVINGER, DM PEOPLES, RW WEIGHT, FF AF WHITE, G LOVINGER, DM PEOPLES, RW WEIGHT, FF TI INHIBITION OF N-METHYL-D-ASPARTATE ACTIVATED ION CURRENT BY DESMETHYLIMIPRAMINE SO BRAIN RESEARCH LA English DT Note DE TRICYCLIC; ANTIDEPRESSANT; NEUROTRANSMITTER; EXCITATORY AMINO ACID; ZINC; ION CHANNEL; N-METHYL-D-ASPARTATE ID TRICYCLIC ANTIDEPRESSANTS; RECEPTOR; BLOCK; PHENCYCLIDINE; GLUTAMATE; KETAMINE; NEURONS; CHANNEL; SITES AB The tricyclic antidepressant desmethylimipramine (DMI) interacts with the NMDA receptor/ionophore complex; however, the site of the interaction has not been clearly established. Although evidence from receptor binding assays suggest that DMA interacts with the Zn2+ binding site, other binding studies and electrophysiological studies suggest otherwise. Using the whole-cell patch clamp technique to record from cultured hippocampal neurons, we report that recovery of NMDA-activated current from block by DMI is time-dependent and this time-dependent was not observed following preexposure of neurons to Zn2+. These observations favor the hypothesis that DMI interacts at a binding site within the NMDA receptor/complex channel pore and not at the Zn2+ binding site. C1 NIAAA,ELECTROPHYSIOL SECT,ROCKVILLE,MD 20852. NR 14 TC 18 Z9 19 U1 3 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD DEC 24 PY 1990 VL 537 IS 1-2 BP 337 EP 339 DI 10.1016/0006-8993(90)90381-K PG 3 WC Neurosciences SC Neurosciences & Neurology GA EP961 UT WOS:A1990EP96100049 PM 1707733 ER PT J AU HUERTA, MF PONS, TP AF HUERTA, MF PONS, TP TI PRIMARY MOTOR CORTEX RECEIVES INPUT FROM AREA 3A IN MACAQUES SO BRAIN RESEARCH LA English DT Note DE PRIMARY MOTOR CORTEX; CORTICAL AREA 3A; SENSORIMOTOR; PRIMATE; CONNECTIONAL TOPOGRAPHY; INTRACORTICAL MICROSTIMULATION ID UNANESTHETIZED MONKEY; SENSORIMOTOR CORTEX; RAMP STRETCH; RESPONSES; NEURONS; FASCICULARIS; ORGANIZATION; HINDLIMB; MUSCLES; FIELDS AB Intracortical microstimulation was used to define topographic sectors and the rostral border of primary motor cortex in adult macaques (Macaca mulatta). In the same animals, injections of fluorescent tracers were made within defined regions of primary motor cortex. Retrogradely labeled neurons were topographically distributed in area 3a, with most neurons located in layer III, and fewer neurons situated in layers V and IV. These findings suggest that muscle afferent information, thought to be important to a closed-loop mode of function, may reach primary motor cortex directly from cortical area 3a. C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. RP HUERTA, MF (reprint author), UNIV CONNECTICUT,CTR HLTH,DEPT BIOSTRUCT & FUNCT,FARMINGTON,CT 06032, USA. FU NINDS NIH HHS [NS25874] NR 26 TC 67 Z9 68 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD DEC 24 PY 1990 VL 537 IS 1-2 BP 367 EP 371 DI 10.1016/0006-8993(90)90388-R PG 5 WC Neurosciences SC Neurosciences & Neurology GA EP961 UT WOS:A1990EP96100056 PM 2085789 ER PT J AU NISHIMORI, T BUZZI, MG CHUDLER, EH POLETTI, CE MOSKOWITZ, MA UHL, GR AF NISHIMORI, T BUZZI, MG CHUDLER, EH POLETTI, CE MOSKOWITZ, MA UHL, GR TI PREPROENKEPHALIN UP-REGULATION IN NUCLEUS CAUDALIS - HIGH AND LOW INTENSITY AFFERENT STIMULATION DIFFERENTIALLY MODULATE EARLY AND LATE RESPONSES SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE INSITU HYBRIDIZATION; PAIN; TRIGEMINAL NERVE; GENE REGULATION ID DORSAL HORN NEURONS; PRIMARY SENSORY NEURONS; LUMBAR SPINAL-CORD; FOS-LIKE PROTEIN; TRIGEMINAL-NUCLEUS; SUBSTANTIA GELATINOSA; INSITU HYBRIDIZATION; PERIPHERAL-NERVE; MESSENGER-RNA; CYCLIC-AMP AB Nucleus caudalis expression of preproenkephalin mRNA changes following lesions depleting small-caliber primary afferent fibers and after stimulation of trigeminal afferents at different intensities. Animals treated neonatally with capsaicin display reduced preproenkephalin gene expression in nucleus caudalis neurons. Stimulation of normal animals at low intensities enhances preproenkephalin expression in a bimodal temporal pattern. High intensity stimulation is effective only at later time points in normal animals, but it causes both early and late effects on preprornkephalin expression when applied to animals neonatally lesioned with capsaicin. Transsynaptic regulation of preproenkephalin expression in pain-modulating areas of the nucleus caudalis of the trigeminal nerve thus depends on the specific type of primary afferent input. The rapid responses noted after selective large fiber stimulation appear to be suppressed by coactivation of small caliber fibers. Later responses appear less influenced by the quality of the eliciting afferent stimulus. C1 JOHNS HOPKINS UNIV, SCH MED, NIDA, ARC, MOLEC NEUROBIOL LAB, BOX 5180, BALTIMORE, MD 21224 USA. MASSACHUSETTS GEN HOSP, DEPT NEUROL, BOSTON, MA 02114 USA. MASSACHUSETTS GEN HOSP, DEPT NEUROSURG, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. JOHNS HOPKINS UNIV, SCH MED, DEPT NEUROL, BALTIMORE, MD 21224 USA. JOHNS HOPKINS UNIV, SCH MED, DEPT NEUROSCI, BALTIMORE, MD 21224 USA. NR 56 TC 18 Z9 18 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-9967 EI 1096-9861 J9 J COMP NEUROL JI J. Comp. Neurol. PD DEC 22 PY 1990 VL 302 IS 4 BP 1002 EP 1018 DI 10.1002/cne.903020422 PG 17 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA EP666 UT WOS:A1990EP66600021 PM 2081812 ER PT J AU ROBSON, KJH HALL, JRS DAVIES, LC CRISANTI, A HILL, AVS WELLEMS, TE AF ROBSON, KJH HALL, JRS DAVIES, LC CRISANTI, A HILL, AVS WELLEMS, TE TI POLYMORPHISM OF THE TRAP GENE OF PLASMODIUM-FALCIPARUM SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article ID HUMAN MALARIA PARASITE; AMINO-ACID-SEQUENCE; T-CELL EPITOPES; CIRCUMSPOROZOITE PROTEIN; VACCINE DEVELOPMENT; DNA-POLYMERASE; THROMBOSPONDIN; FRAGMENTS; VIRUS; GLYCOPROTEIN AB Natural sequence variation of the thrombospondin related anonymous protein (TRAP) gene of Plasmodium falciparum has been investigated by DNA analysis following the polymerase chain reaction amplification, and this shows the gene to be highly polymorphic. The region containing the sequence motif Trp-Ser-Pro-Cys-Ser- Val-Thr-Cys-Gly (WSPCSVTCG), common to TRAP, the circumsporozoite protein, properdin, and thrombospondin, was invariant. Elsewhere in the molecule, over 50 amino acid substitutions are described including the insertion of an in-frame, small-variable tandemly repeating motif between amino acid residues 352 and 353. Only one silent mutation was observed. Most nucleotide changes that occur in the first two codon positions result in conservative amino acid changes. Restriction fragment length polymorphism (RFLP) analysis was used to examine inheritance of TRAP in a cross between the HB3 and 3D7 clones of P. falciparum. Out of nine progeny examined, four possessed the HB3 gene and five the 3D7 gene. The TRAP gene hybridized to chromosome 13. Previous work has shown that a subtelomeric region of chromosome 13 from the 3D7 parent (marked by the HRP-III gene) was favoured strongly in this cross. The TRAP gene, however, is over 1 Mb away from this subtelomeric region and exhibits no such linkage because of chromosome crossovers. Five geographically separate isolates shared the same TRAP sequence as well as the same variant of the Th2R/Th3R region from the circumsporozoite protein. The correlation between independent markers in these isolates suggests that they have a common provenance. C1 UNIV ROME LA SAPIENZA, IST PARASITOL, I-00184 ROME, ITALY. JOHN RADCLIFFE HOSP, INST MOLEC MED, FOREIGN AGR GRP, OXFORD OX3 9DU, ENGLAND. NIAID, MALARIA SECT, BETHESDA, MD 20892 USA. RP JOHN RADCLIFFE HOSP, INST MOLEC MED, MRC, MOLEC HAEMATOL UNIT, OXFORD OX3 9DU, ENGLAND. RI HILL, Adrian/C-1306-2008 FU Wellcome Trust NR 36 TC 71 Z9 72 U1 0 U2 0 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD DEC 22 PY 1990 VL 242 IS 1305 BP 205 EP 216 DI 10.1098/rspb.1990.0126 PG 12 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA ET336 UT WOS:A1990ET33600010 PM 1983036 ER PT J AU SCHEFFNER, M WERNESS, BA HUIBREGTSE, JM LEVINE, AJ HOWLEY, PM AF SCHEFFNER, M WERNESS, BA HUIBREGTSE, JM LEVINE, AJ HOWLEY, PM TI THE E6 ONCOPROTEIN ENCODED BY HUMAN PAPILLOMAVIRUS TYPE-16 AND TYPE-18 PROMOTES THE DEGRADATION OF P53 SO CELL LA English DT Article ID CELLULAR TUMOR-ANTIGEN; RETINOBLASTOMA GENE-PRODUCT; ADENOVIRUS E1A PROTEINS; TRANSFORMED-CELLS; SV40-TRANSFORMED CELLS; COLORECTAL CARCINOMAS; T-ANTIGEN; ONCOGENE; IMMORTALIZATION; IDENTIFICATION AB The E6 protein encoded by the oncogenic human papillomavirus types 16 and 18 is one of two viral products expressed in HPV-associated cancers. E6 is an oncoprotein which cooperates with E7 to immortalize primary human keratinocytes. Insight into the mechanism by which E6 functions in oncogenesis is provided by the observation that the E6 protein encoded by HPV-16 and HPV-18 can complex the wild-type p53 protein in vitro. Wild-type p53 gene has tumor suppressor properties, and is a target for several of the oncoproteins encoded by DNA tumor viruses. In this study we demonstrate that the E6 proteins of the oncogenic HPVs that bind p53 stimulate the degradation of p53. The E6-promoted degradation of p53 is ATP dependent and involves the ubiquitin-dependent protease system. Selective degradation of cellular proteins such as p53 with negative regulatory functions provides a novel mechanism of action for dominant-acting oncoproteins. C1 PRINCETON UNIV,DEPT BIOL,PRINCETON,NJ 08544. RP SCHEFFNER, M (reprint author), NCI,TUMOR VIRUS BIOL LAB,BETHESDA,MD 20892, USA. RI Scheffner, Martin/K-2940-2012; Tang, Amy/L-3226-2016 OI Scheffner, Martin/0000-0003-2229-0128; Tang, Amy/0000-0002-5772-2878 NR 54 TC 3047 Z9 3100 U1 16 U2 117 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD DEC 21 PY 1990 VL 63 IS 6 BP 1129 EP 1136 DI 10.1016/0092-8674(90)90409-8 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA EN923 UT WOS:A1990EN92300004 PM 2175676 ER PT J AU SAAVEDRA, JE AF SAAVEDRA, JE TI DECOMPOSITION OF 1-(NITROSOALKYL)-3-(2-HYDROXYALKYL)UREAS SO JOURNAL OF ORGANIC CHEMISTRY LA English DT Article ID RATS; CARCINOGENESIS; NITROSOUREAS; DERIVATIVES; ALKYLATION; MECHANISM; DNA RP SAAVEDRA, JE (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21701, USA. NR 16 TC 2 Z9 2 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-3263 J9 J ORG CHEM JI J. Org. Chem. PD DEC 21 PY 1990 VL 55 IS 26 BP 6373 EP 6374 DI 10.1021/jo00313a027 PG 2 WC Chemistry, Organic SC Chemistry GA EP605 UT WOS:A1990EP60500027 ER PT J AU JONES, LA CHIN, LT LONGO, DL KRUISBEEK, AM AF JONES, LA CHIN, LT LONGO, DL KRUISBEEK, AM TI PERIPHERAL CLONAL ELIMINATION OF FUNCTIONAL T-CELLS SO SCIENCE LA English DT Article RP NCI, BIOL RESPONSE MODIFIERS PROGRAM, BETHESDA, MD 20892 USA. NR 62 TC 231 Z9 234 U1 0 U2 0 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD DEC 21 PY 1990 VL 250 IS 4988 BP 1726 EP 1729 DI 10.1126/science.2125368 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EN920 UT WOS:A1990EN92000046 PM 2125368 ER PT J AU COLLIN, C PAPAGEORGE, AG LOWY, DR ALKON, DL AF COLLIN, C PAPAGEORGE, AG LOWY, DR ALKON, DL TI EARLY ENHANCEMENT OF CALCIUM CURRENTS BY H-RAS ONCOPROTEINS INJECTED INTO HERMISSENDA NEURONS SO SCIENCE LA English DT Article C1 NCI, CELLULAR ONCOL LAB, FREDERICK, MD 21701 USA. RP NINCDS, MOLEC & CELLULAR NEUROBIOL LAB, NEURAL SYST SECT, BETHESDA, MD 20892 USA. NR 35 TC 53 Z9 53 U1 0 U2 0 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD DEC 21 PY 1990 VL 250 IS 4988 BP 1743 EP 1745 DI 10.1126/science.2176747 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EN920 UT WOS:A1990EN92000052 PM 2176747 ER PT J AU LAROCCA, RV STEIN, CA DANESI, R MYERS, CE AF LAROCCA, RV STEIN, CA DANESI, R MYERS, CE TI SURAMIN, A NOVEL ANTITUMOR COMPOUND SO JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article; Proceedings Paper CT 2ND INTERNATIONAL SYMP ON HORMONAL MANIPULATION OF CANCER : PEPTIDES, GROWTH FACTORS AND NEW ( ANTI- ) STEROIDAL AGENTS CY APR 09-11, 1990 CL ROTTERDAM, NETHERLANDS SP EUROPEAN ORG RES TREATMENT CANC, DUTCH CANC SOC, DR DANIEL DEN HOED CANC CTR, NEFKENS FDN ID HTLV-III/LAV INFECTION; GROWTH-FACTOR; SARCOMA-VIRUS; CELLS; INHIBITION; CANCER; DRUG AB Suramin, a polyanionic compound originally synthesized for use as an antiparasitic agent, has recently entered clinical trials for the treatment of a variety of human cancers refractory to conventional modalities of therapy. This is based on suramin's ability to bind and to inactivate growth factor and enzyme systems critical to cellular homeostasis and proliferation. In addition, this compound possesses adrenocorticolytic properties in vivo and exerts significant cytostatic and cytocidal effects against a variety of human tumor cell lines in vitro. Pilot studies using suramin have thus far been conducted in adrenocortical carcinoma, prostate cancer refractory to conventional hormonal manipulation and nodular lymphomas. RP LAROCCA, RV (reprint author), NCI,MED BRANCH,CLIN ONCOL PROGRAM,BETHESDA,MD 20892, USA. NR 33 TC 53 Z9 53 U1 6 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0960-0760 J9 J STEROID BIOCHEM JI J. Steroid Biochem. Mol. Biol. PD DEC 20 PY 1990 VL 37 IS 6 BP 893 EP 898 DI 10.1016/0960-0760(90)90439-R PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA EX990 UT WOS:A1990EX99000026 PM 2285603 ER PT J AU SRIVASTAVA, S ZOU, ZQ PIROLLO, K BLATTNER, W CHANG, EH AF SRIVASTAVA, S ZOU, ZQ PIROLLO, K BLATTNER, W CHANG, EH TI GERM-LINE TRANSMISSION OF A MUTATED P53 GENE IN A CANCER-PRONE FAMILY WITH LI-FRAUMENI SYNDROME SO NATURE LA English DT Article C1 NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. UNIFORMED SERV UNIV HLTH SCI,DEPT SURG,BETHESDA,MD 20814. RP SRIVASTAVA, S (reprint author), UNIFORMED SERV UNIV HLTH SCI,DEPT PATHOL,4301 JONES BRIDGE RD,BETHESDA,MD 20814, USA. NR 27 TC 1013 Z9 1025 U1 3 U2 22 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD DEC 20 PY 1990 VL 348 IS 6303 BP 747 EP 749 DI 10.1038/348747a0 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EP049 UT WOS:A1990EP04900065 PM 2259385 ER PT J AU REITMAN, M LEE, E WESTPHAL, H FELSENFELD, G AF REITMAN, M LEE, E WESTPHAL, H FELSENFELD, G TI SITE-INDEPENDENT EXPRESSION OF THE CHICKEN BETA-A-GLOBIN GENE IN TRANSGENIC MICE SO NATURE LA English DT Article C1 NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. RP REITMAN, M (reprint author), NIDDKD,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. RI Reitman, Marc/B-4448-2013 OI Reitman, Marc/0000-0002-0426-9475 NR 29 TC 90 Z9 91 U1 0 U2 0 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD DEC 20 PY 1990 VL 348 IS 6303 BP 749 EP 752 DI 10.1038/348749a0 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EP049 UT WOS:A1990EP04900066 PM 2175398 ER PT J AU ANHALT, GJ KIM, S STANLEY, JR KORMAN, NJ JABS, DA KORY, M IZUMI, H RATRIE, H MUTASIM, D ARISSABDO, L LABIB, RS AF ANHALT, GJ KIM, S STANLEY, JR KORMAN, NJ JABS, DA KORY, M IZUMI, H RATRIE, H MUTASIM, D ARISSABDO, L LABIB, RS TI PARANEOPLASTIC PEMPHIGUS - AN AUTOIMMUNE MUCOCUTANEOUS DISEASE ASSOCIATED WITH NEOPLASIA SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article C1 JOHNS HOPKINS UNIV,SCH MED,DEPT OPHTHALMOL,BALTIMORE,MD 21205. NCI,DERMATOL BRANCH,BETHESDA,MD 20892. OHIO STATE UNIV,COLUMBUS,OH 43210. RP ANHALT, GJ (reprint author), JOHNS HOPKINS UNIV,SCH MED,DEPT DERMATOL,600 N WOLFE ST,BALTIMORE,MD 21205, USA. FU NIAMS NIH HHS [R01-AR-32490, KO4-AR-01686] NR 26 TC 632 Z9 678 U1 1 U2 7 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD DEC 20 PY 1990 VL 323 IS 25 BP 1729 EP 1735 DI 10.1056/NEJM199012203232503 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA EN375 UT WOS:A1990EN37500003 PM 2247105 ER PT J AU BENNETT, JE AF BENNETT, JE TI SEARCHING FOR THE YEAST CONNECTION SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Editorial Material RP BENNETT, JE (reprint author), NIAID,BETHESDA,MD 20892, USA. NR 9 TC 12 Z9 12 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD DEC 20 PY 1990 VL 323 IS 25 BP 1766 EP 1767 DI 10.1056/NEJM199012203232509 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA EN375 UT WOS:A1990EN37500009 PM 2247107 ER PT J AU PANZA, JA AF PANZA, JA TI ABNORMAL VASCULAR RELAXATION IN ESSENTIAL-HYPERTENSION - REPLY SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter RP PANZA, JA (reprint author), NHLBI,BETHESDA,MD 20812, USA. NR 6 TC 0 Z9 0 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD DEC 20 PY 1990 VL 323 IS 25 BP 1773 EP 1773 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EN375 UT WOS:A1990EN37500024 ER PT J AU STERN, MH AF STERN, MH TI ATAXIA TELANGIECTASIA - A MODEL OF INVESTIGATION OF CHROMOSOMAL TRANSLOCATIONS SO SEMAINE DES HOPITAUX LA French DT Article RP STERN, MH (reprint author), NCI,NAVAL HOSP,NAVAL MED ONCOL BRANCH,BLDG 8 ROOM 5101,BETHESDA,MD 20814, USA. RI Stern, Marc-Henri/A-2728-2011 OI Stern, Marc-Henri/0000-0002-8100-2272 NR 0 TC 0 Z9 0 U1 0 U2 1 PU EXPANSION SCI FRANCAISE PI PARIS PA 31 BLVD LATOUR MAUBOURG, 75007 PARIS, FRANCE SN 0037-1777 J9 SEM HOP PARIS JI Sem. Hop. PD DEC 20 PY 1990 VL 66 IS 48-49 BP 2744 EP 2746 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA EP925 UT WOS:A1990EP92500007 ER PT J AU DAVIS, CE RIFKIND, BM BRENNER, H GORDON, DJ AF DAVIS, CE RIFKIND, BM BRENNER, H GORDON, DJ TI A SINGLE CHOLESTEROL MEASUREMENT UNDERESTIMATES THE RISK OF CORONARY HEART-DISEASE - AN EMPIRICAL EXAMPLE FROM THE LIPID RESEARCH CLINICS MORTALITY FOLLOW-UP-STUDY SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Note C1 NIH,BETHESDA,MD 20892. UNIV ULM,W-7900 ULM,GERMANY. RP DAVIS, CE (reprint author), UNIV N CAROLINA,DEPT BIOSTAT,COLLABORAT STUDIES COORDINATING CTR,SUITE 203,NCNB PLAZA,CHAPEL HILL,NC 27514, USA. RI Brenner, Hermann/B-4627-2017 OI Brenner, Hermann/0000-0002-6129-1572 FU NHLBI NIH HHS [N01-HV12159, N01-HV12156, N01-HV12160] NR 13 TC 100 Z9 103 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 19 PY 1990 VL 264 IS 23 BP 3044 EP 3046 DI 10.1001/jama.264.23.3044 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA EM763 UT WOS:A1990EM76300029 PM 2243433 ER PT J AU RIFKIND, BM GROUSE, LD AF RIFKIND, BM GROUSE, LD TI CHOLESTEROL REDUX SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material C1 MED COMMUN RESOURCES INC,SANTA MONICA,CA. RP RIFKIND, BM (reprint author), NHLBI,DIV HEART & VASC DIS,LIPID METAB ATHEROGENESIS BRANCH,FED BLDG,BETHESDA,MD 20892, USA. NR 21 TC 3 Z9 3 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 19 PY 1990 VL 264 IS 23 BP 3060 EP 3061 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA EM763 UT WOS:A1990EM76300032 PM 2243436 ER PT J AU GOLDSTEIN, AM AMOS, CI AF GOLDSTEIN, AM AMOS, CI TI SEGREGATION ANALYSIS OF BREAST-CANCER FROM THE CANCER AND STEROID-HORMONE STUDY - HISTOLOGIC SUBTYPES SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article C1 NCI,ENVIRONM EPIDEMIOL BRANCH,FAMILY STUDIES SECT,BETHESDA,MD 20892. FU NICHD NIH HHS [3-Y01-HD-8-1037] NR 41 TC 13 Z9 13 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD DEC 19 PY 1990 VL 82 IS 24 BP 1911 EP 1917 DI 10.1093/jnci/82.24.1911 PG 7 WC Oncology SC Oncology GA EN053 UT WOS:A1990EN05300014 PM 2174465 ER PT J AU TOMASZEWSKI, KE HEINDEL, SW JENKINS, WL MELNICK, RL AF TOMASZEWSKI, KE HEINDEL, SW JENKINS, WL MELNICK, RL TI INDUCTION OF PEROXISOMAL ACYL COA OXIDASE ACTIVITY AND LIPID-PEROXIDATION IN PRIMARY RAT HEPATOCYTE CULTURES SO TOXICOLOGY LA English DT Article DE PEROXISOME; HEPATOCYTES; LIPID PEROXIDATION; NAFENOPIN; CLOFIBRIC ACID; MONO(2-ETHYL-HEXYL)PHTHALATE ID BETA-OXIDATION; CLOFIBRIC ACID; F344 RATS; PROLIFERATORS; LIVER; DI(2-ETHYLHEXYL)PHTHALATE; PHTHALATE; DNA; MITOCHONDRIAL; METABOLITES AB Peroxisome proliferators have been suggested to induce liver carcinogenesis as a result of increased peroxisomal hydrogen peroxide production and cellular oxidative stress. Primary monolayer cultures of hepatocytes isolated from male F344 rats were incubated in medium containing one of three different peroxisome proliferators and examined for the induction of peroxisomal CoA oxidase activity and lipid peroxidation. The latter parameter was determined by measuring levels of conjugated dienes in lipid fractions extracted from harvested cells. The peroxisome proliferators used in these studies were nafenopin and clofibric acid (two hypolipidemic drugs) and mono(2-ethylhexyl)phthalate (MEHP), the primary metabolite of the industrial plasticizer, di(2-ethylhexyl)phthalate (DEHP). The relative specific activity of peroxisomal acyl CoA oxidase was increased by about 300% after incubation for 44 h with 200-mu-M nafenopin; lower levels of induction were observed with clofibric acid or MEHP. Relative to controls, the level of conjugated dienes was increased approximately 2-fold after incubation with 200-mu-M nafenopin; there was no apparent increase in conjugated dienes after incubation with up to 200-mu-M MEHP or 400-mu-M clofibric acid. The increase in conjugated dienes with 200-mu-M nafenopin was inhibited by co-incubation with the antioxidant, N,N'-diphenyl-p-phenylenediamine. Thus, peroxisomal enzyme induction by nafenopin can result in membrane lipid peroxidation and monolayer cultures of rat hepatocytes may provide a useful model system for studying relationships between peroxisome proliferation, enhanced hydrogen peroxide production and cellular changes due to hepatic oxidative stress. C1 NIEHS,POB 12233,RES TRIANGLE PK,NC 27709. NR 37 TC 23 Z9 23 U1 1 U2 2 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PD DEC 17 PY 1990 VL 65 IS 1-2 BP 49 EP 60 DI 10.1016/0300-483X(90)90078-U PG 12 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA ET817 UT WOS:A1990ET81700004 PM 2274969 ER PT J AU STOHS, SJ ABBOTT, BD LIN, FH BIRNBAUM, LS AF STOHS, SJ ABBOTT, BD LIN, FH BIRNBAUM, LS TI INDUCTION OF ETHOXYRESORUFIN-O-DEETHYLASE AND INHIBITION OF GLUCOCORTICOID RECEPTOR-BINDING IN SKIN AND LIVER OF HAIRED AND HAIRLESS HRS/J MICE BY TOPICALLY APPLIED 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN SO TOXICOLOGY LA English DT Article DE 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN (TCDD); ETHOXYRESORUFIN-O-DEETHYLASE (EROD); GLUCOCORTICOID RECEPTORS; EPIDERMAL GROWTH FACTOR (EGF) RECEPTORS; SKIN; HAIRLESS MICE ID ARYL-HYDROCARBON HYDROXYLASE; AH LOCUS; MOUSE; TOXICITY; TCDD; RAT; "2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN; CHROMOSOME-12; EPIDERMIS; DECREASES AB The biochemical changes associated with the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) have been reported to include alterations in glucocorticoid and epidermal growth factor receptors and mixed function oxidase (MFO) induction. TCDD induces MFO activity in skin of both haired and hairless HRS/J mice. However, epidermal hyperplasia and hyperkeratosis are produced only in the skin of hairless mice. Therefore, since steroid and growth factor responses are implicated in cell proliferation and differentiation, these mice constitute a model system for assessing the possible roles of glucocorticoid and epidermal growth factor receptors in the toxicity of TCDD. The effect of dermal TCDD application (12-mu-g/kg in 100-mu-l acetone) on ethoxyresorufin-O-deethylase (EROD) activity, glucocorticoid receptor binding and epidermal growth factor receptors in liver and skin of hairless and haired mice was determined. No differences existed in the basal number of cytosolic glucocorticoid receptors (B(max)) or the apparent equilibrium binding constants (K(d)) in control liver, dorsal skin and abdominal skin of male and female hairless mice and haired male mice. Seven days after topical application of TCDD, decreases of approximately 38% were observed in the hepatic B(max) of the glucocorticoid receptors in both haired and hairless mice. However, in dorsal skin, TCDD decreased B(max) by approximately 40% in hairless mice but only 18% in haired mice. The dexamethasone-glucocorticoid receptor complex from both liver and skin of control and TCDD treated mice had similar sedimentation co-efficients in sucrose density gradients. TCDD had no effect on the K(d) of glucocorticoid receptors of skin or liver in haired and hairless mice. No difference was observed in the time-dependent increases in hepatic EROD activity between haired and hairless mice after dermal application of TCDD. However, the maximum induction of EROD activity in microsomes from the skin of haired mice was only 60% of the activity observed in hairless animals. The induction of EROD by TCDD did not correlate temporally with the decrease in glucocorticoid receptor binding. The application of TCDD to the skin of hairless mice resulted in epidermal hyperplasia and dermal keratinization, while little change was observed in the general morphology of the skin of haired mice following dermal application. The application of TCDD had no effect on the incidence and distribution of epidermal growth factor receptors in skin of haired and hairless mice as determined immunohistochemically. Thus, the biochemical effects of TCDD are not only strain dependent, but tissue specific. Furthermore, decreases in glucocorticoid and epidermal growth factor receptors do not appear to be general markers of TCDD toxicity. C1 NIEHS,SYST TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. NIEHS,BIOCHEM RISK ASSESSMENT LAB,RES TRIANGLE PK,NC 27709. NR 33 TC 16 Z9 16 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0300-483X J9 TOXICOLOGY JI Toxicology PD DEC 17 PY 1990 VL 65 IS 1-2 BP 123 EP 136 DI 10.1016/0300-483X(90)90083-S PG 14 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA ET817 UT WOS:A1990ET81700009 PM 2274963 ER PT J AU ROJAS, E CENA, V STUTZIN, A FORSBERG, E POLLARD, HB AF ROJAS, E CENA, V STUTZIN, A FORSBERG, E POLLARD, HB TI CHARACTERISTICS OF RECEPTOR-OPERATED AND MEMBRANE POTENTIAL-DEPENDENT ATP SECRETION FROM ADRENAL-MEDULLARY CHROMAFFIN CELLS SO ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article ID CATECHOLAMINE SECRETION; PROTEIN-PHOSPHORYLATION; RELEASE; EXOCYTOSIS; TRIFLUOPERAZINE; INHIBITION; SYNEXIN RP ROJAS, E (reprint author), NIDDKD, CELL BIOL & GENET LAB, BETHESDA, MD 20892 USA. NR 22 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 E 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PD DEC 16 PY 1990 VL 603 BP 311 EP 323 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EZ861 UT WOS:A1990EZ86100026 ER PT J AU BREZIN, AP EGWUAGU, CE BURNIER, M SILVEIRA, C MAHDI, RM GAZZINELLI, RT BELFORT, R NUSSENBLATT, RB AF BREZIN, AP EGWUAGU, CE BURNIER, M SILVEIRA, C MAHDI, RM GAZZINELLI, RT BELFORT, R NUSSENBLATT, RB TI IDENTIFICATION OF TOXOPLASMA-GONDII IN PARAFFIN-EMBEDDED SECTIONS BY THE POLYMERASE CHAIN-REACTION SO AMERICAN JOURNAL OF OPHTHALMOLOGY LA English DT Article C1 NEI,IMMUNOL LAB,BLDG 10,RM 10N202,BETHESDA,MD 20892. BRAZILIAN REGISTRY OPHTHALM PATHOL,SAO PAULO,BRAZIL. CLIN SILVEIRA,EREXIM,BRAZIL. ARMED FORCES INST PATHOL,DEPT OPHTHALM PATHOL,WASHINGTON,DC 20306. NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. ESCOLA PAULISTA MED SCH,BR-04023 SAO PAULO,BRAZIL. RI Belfort Jr, Rubens/E-2252-2012 OI Belfort Jr, Rubens/0000-0002-8422-3898 NR 27 TC 46 Z9 49 U1 0 U2 0 PU OPHTHALMIC PUBL CO PI CHICAGO PA 77 WEST WACKER DR, STE 660, CHICAGO, IL 60601 SN 0002-9394 J9 AM J OPHTHALMOL JI Am. J. Ophthalmol. PD DEC 15 PY 1990 VL 110 IS 6 BP 599 EP 604 PG 6 WC Ophthalmology SC Ophthalmology GA EL935 UT WOS:A1990EL93500002 PM 2248322 ER PT J AU BELFORT, R NUSSENBLATT, RB LOTTEMBERG, C KWITKO, S CHAMON, W DESMET, M RUBIN, B AF BELFORT, R NUSSENBLATT, RB LOTTEMBERG, C KWITKO, S CHAMON, W DESMET, M RUBIN, B TI SPONTANEOUS LENS SUBLUXATION IN UVEITIS SO AMERICAN JOURNAL OF OPHTHALMOLOGY LA English DT Letter C1 HOSP SAO PAULO,ESCOLA PAULISTA MED,SAO PAULO,BRAZIL. RP BELFORT, R (reprint author), NEI,RM 10N202,BLDG 10,BETHESDA,MD 20892, USA. RI Belfort Jr, Rubens/E-2252-2012; Chamon, Wallace/A-5400-2010; OI Belfort Jr, Rubens/0000-0002-8422-3898; Chamon, Wallace/0000-0002-2528-0526; de Smet, Marc/0000-0002-9217-5603 NR 4 TC 7 Z9 7 U1 0 U2 0 PU OPHTHALMIC PUBL CO PI CHICAGO PA 77 WEST WACKER DR, STE 660, CHICAGO, IL 60601 SN 0002-9394 J9 AM J OPHTHALMOL JI Am. J. Ophthalmol. PD DEC 15 PY 1990 VL 110 IS 6 BP 714 EP 716 PG 3 WC Ophthalmology SC Ophthalmology GA EL935 UT WOS:A1990EL93500027 PM 2248347 ER PT J AU NEWBURY, DE FIORI, CE MARINENKO, RB MYKLEBUST, RL SWYT, CR BRIGHT, DS AF NEWBURY, DE FIORI, CE MARINENKO, RB MYKLEBUST, RL SWYT, CR BRIGHT, DS TI COMPOSITIONAL MAPPING WITH THE ELECTRON-PROBE MICROANALYZER .2. SO ANALYTICAL CHEMISTRY LA English DT Article C1 NIH,BIOMED ENGN RES BRANCH,BIOL ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. RP NEWBURY, DE (reprint author), NIST,CTR ANALYT CHEM,MICROANAL RES GRP,GAITHERSBURG,MD 20899, USA. NR 23 TC 14 Z9 14 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0003-2700 J9 ANAL CHEM JI Anal. Chem. PD DEC 15 PY 1990 VL 62 IS 24 BP A1245 EP & PG 0 WC Chemistry, Analytical SC Chemistry GA EN073 UT WOS:A1990EN07300002 ER PT J AU FRICKHOFEN, N ABKOWITZ, JL SAFFORD, M BERRY, JM ANTUNEZDEMAYOLO, J ASTROW, A COHEN, R HALPERIN, I KING, L MINTZER, D COHEN, B YOUNG, NS AF FRICKHOFEN, N ABKOWITZ, JL SAFFORD, M BERRY, JM ANTUNEZDEMAYOLO, J ASTROW, A COHEN, R HALPERIN, I KING, L MINTZER, D COHEN, B YOUNG, NS TI PERSISTENT B19 PARVOVIRUS INFECTION IN PATIENTS INFECTED WITH HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) - A TREATABLE CAUSE OF ANEMIA IN AIDS SO ANNALS OF INTERNAL MEDICINE LA English DT Article C1 NHLBI,BLDG 10-7C103,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. UNIV WASHINGTON,SCH MED,SEATTLE,WA 98195. BROWN UNIV,MIRIAM HOSP,PROVIDENCE,RI 02912. PACIFIC PRESBYTERIAN MED CTR,SAN FRANCISCO,CA. UNIV MIAMI,SCH MED,MIAMI,FL 33152. ST VINCENTS HOSP & MED CTR,NEW YORK,NY 10011. PENN HOSP,PHILADELPHIA,PA 19107. CENT PUBL HLTH LAB,LONDON NW9 5HT,ENGLAND. NR 40 TC 361 Z9 366 U1 0 U2 5 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD DEC 15 PY 1990 VL 113 IS 12 BP 926 EP 933 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA EM297 UT WOS:A1990EM29700006 PM 2173460 ER PT J AU OGNIBENE, FP CUNNION, RE PARRILLO, JE AF OGNIBENE, FP CUNNION, RE PARRILLO, JE TI FLUID LOADING IN SEPTIC SHOCK - RESPONSE SO ANNALS OF INTERNAL MEDICINE LA English DT Letter C1 RUSH PRESBYTERIAN ST LUKES MED CTR,CHICAGO,IL 60612. RP OGNIBENE, FP (reprint author), NIH,BETHESDA,MD 20892, USA. NR 4 TC 0 Z9 0 U1 0 U2 0 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD DEC 15 PY 1990 VL 113 IS 12 BP 991 EP 992 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA EM297 UT WOS:A1990EM29700017 ER PT J AU KAUFMAN, EE PORRINO, LJ NELSON, T AF KAUFMAN, EE PORRINO, LJ NELSON, T TI PYRETIC ACTION OF LOW-DOSES OF GAMMA-HYDROXYBUTYRATE IN RATS SO BIOCHEMICAL PHARMACOLOGY LA English DT Article ID SODIUM 4-HYDROXYBUTYRATE; METHOHEXITONE; DIAZEPAM AB Gamma-hydroxybutyrate (GHB) has been found to have a biphasic effect on body temperature with increased body temperature after low doses (5-10 mg/kg) and decreased body temperature after high doses (300-500 mg/kg). Brain levels of GHB between 30 and 60 min post-injection of GHB were not altered by the low doses (5-10 mg/kg), although a dose of 200 mg/kg produced a large increase in the brain concentration. RP KAUFMAN, EE (reprint author), US DEPT HHS,NIMH,CEREBRAL METAB LAB,BLDG 36,ROOM 1A-05,BETHESDA,MD 20892, USA. NR 17 TC 35 Z9 35 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD DEC 15 PY 1990 VL 40 IS 12 BP 2637 EP 2640 DI 10.1016/0006-2952(90)90581-5 PG 4 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EP086 UT WOS:A1990EP08600009 PM 2260987 ER PT J AU BOARMAN, DM BARAM, J ALLEGRA, CJ AF BOARMAN, DM BARAM, J ALLEGRA, CJ TI MECHANISM OF LEUCOVORIN REVERSAL OF METHOTREXATE CYTOTOXICITY IN HUMAN MCF-7 BREAST-CANCER CELLS SO BIOCHEMICAL PHARMACOLOGY LA English DT Article ID INTRACELLULAR FOLATE POOLS; MYELOID PRECURSOR CELLS; HUMAN-BONE MARROW; DIHYDROFOLATE-REDUCTASE; THYMIDYLATE SYNTHETASE; DIRECT INHIBITION; PURINE SYNTHESIS; LEUKEMIA-CELLS; CYTO-TOXICITY; L1210 CELLS AB Previous studies have suggested that metabolic inhibition by methotrexate (MTX) is multifactorial and that cytotoxicity can be reversed by the reduced folate leucovorin. In this report we investigated the mechanism of leucovorin rescue in the MCF-7 human breast cancer cell line. Cells were exposed to various concentrations of MTX (0.5, 1.0, 3.0, and 10.0-mu-M) for 24 hr followed by rescue with labelled leucovorin (0.5 to 50-mu-M). The changes in the intracellular folate pools 24 hr following the addition of leucovorin were quantitated by high-pressure liquid chromatographic methods. The changes in the folate pools during rescue were compared with the ability of various concentrations of leucovorin to affect cellular rescue from MTX using a cloning assay. Our studies show that the total labelled intracellular folate pools increased in a log-linear fashion with respect to leucovorin exposure concentrations up to 100-mu-M. The degree of accumulation at a given leucovorin concentration was not significantly different in the absence or presence of MTX over the concentration range of 0.5 to 10-mu-M. Individual folate pool levels (tetrahydrofolate, 10-formyl tetrahydrofolate, 5-formyl tetrahydrofolate, 5-methyl tetrahydrofolate, and 5,10-methylene tetrahydrofolate) reached those present in cells not exposed to MTX at concentrations of leucovorin that were not adequate to rescue the MTX-treated cells. With exposure to concentrations of leucovorin capable of rescue, the individual folate pool levels were up to twelve times greater than those found in untreated cells. consistent with competition for catalytic activity at folate-dependent enzymes in addition to dihydrofolate reductase. The dihydrofolate pool also increased with increasing leucovorin concentration; but, unlike the reduced folates, this oxidized folate reached a maximal level that was dependent on the MTX concentration to which the cells had been exposed. This suggests that competition between MTX and leucovorin occurs at the level of dihydrofolate reductase via a competitive interaction with dihydrofolate in this intact cell system. The ability of leucovorin and its metabolites to compete with direct inhibitors or dihydrofolate reductase and other metabolically important folate-dependent enzymes appears to be associated with leucovorin rescue. C1 NCI,DIV CANC TREATMENT,MED BRANCH,BLDG 10,ROOM 12N226,BETHESDA,MD 20892. NR 44 TC 10 Z9 11 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD DEC 15 PY 1990 VL 40 IS 12 BP 2651 EP 2660 DI 10.1016/0006-2952(90)90583-7 PG 10 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA EP086 UT WOS:A1990EP08600011 PM 2260989 ER PT J AU STEIN, MB UHDE, TW AF STEIN, MB UHDE, TW TI TRIIODOTHYRONINE POTENTIATION OF TRICYCLIC ANTIDEPRESSANT TREATMENT IN PATIENTS WITH PANIC DISORDER SO BIOLOGICAL PSYCHIATRY LA English DT Note ID THYROID-HORMONE; DEPRESSION; AGORAPHOBIA C1 NIMH,BIOL PSYCHIAT BRANCH,ANXIETY & AFFECT DISORDERS SECT,BETHESDA,MD 20892. NR 15 TC 6 Z9 6 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD DEC 15 PY 1990 VL 28 IS 12 BP 1061 EP 1064 DI 10.1016/0006-3223(90)90608-5 PG 4 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA EP894 UT WOS:A1990EP89400007 PM 2289001 ER PT J AU ELMALLAKH, RS CEARNAL, LR KIRCH, DG AF ELMALLAKH, RS CEARNAL, LR KIRCH, DG TI STEREOTYPIC BEHAVIOR AND HYPONATREMIA SO BIOLOGICAL PSYCHIATRY LA English DT Letter RP ELMALLAKH, RS (reprint author), NIMH,NEUROPSYCHIAT RES HOSP,2700 MARTIN LUTHER KING JR AVE SE,WASHINGTON,DC 20032, USA. NR 5 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD DEC 15 PY 1990 VL 28 IS 12 BP 1077 EP 1078 DI 10.1016/0006-3223(90)90618-C PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA EP894 UT WOS:A1990EP89400017 PM 2289006 ER PT J AU MUSSO, T ESPINOZADELGADO, I PULKKI, K GUSELLA, GL LONGO, DL VARESIO, L AF MUSSO, T ESPINOZADELGADO, I PULKKI, K GUSELLA, GL LONGO, DL VARESIO, L TI TRANSFORMING GROWTH-FACTOR-BETA DOWN-REGULATES INTERLEUKIN-1 (IL-1)-INDUCED IL-6 PRODUCTION BY HUMAN MONOCYTES SO BLOOD LA English DT Note C1 NCI,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21701. RP MUSSO, T (reprint author), NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS DEV PROGRAM,FREDERICK,MD 21702, USA. RI varesio, luigi/J-8261-2016 OI varesio, luigi/0000-0001-5659-2218 FU NCI NIH HHS [N01-CO-74102] NR 33 TC 73 Z9 74 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD DEC 15 PY 1990 VL 76 IS 12 BP 2466 EP 2469 PG 4 WC Hematology SC Hematology GA EM901 UT WOS:A1990EM90100007 PM 2265243 ER PT J AU GARBER, JE BURKE, EM LAVALLY, BL BILLETT, AL SALLAN, SE SCOTT, RM KUPSKY, W LI, FP AF GARBER, JE BURKE, EM LAVALLY, BL BILLETT, AL SALLAN, SE SCOTT, RM KUPSKY, W LI, FP TI CHOROID-PLEXUS TUMORS IN THE BREAST-CANCER SARCOMA SYNDROME SO CANCER LA English DT Article C1 NCI,CLIN EPIDEMIOL BRANCH,CLIN STUDIES SECT,BETHESDA,MD 20892. CHILDRENS HOSP MED CTR,BOSTON,MA 02115. RP GARBER, JE (reprint author), HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BRAIN TUMOR WORKING GRP,44 BINNEY ST,BOSTON,MA 02115, USA. NR 18 TC 30 Z9 30 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD DEC 15 PY 1990 VL 66 IS 12 BP 2658 EP 2660 DI 10.1002/1097-0142(19901215)66:12<2658::AID-CNCR2820661232>3.0.CO;2-C PG 3 WC Oncology SC Oncology GA EN145 UT WOS:A1990EN14500031 PM 2249207 ER PT J AU GOFFMAN, TE RAUBITSCHEK, A MITCHELL, JB GLATSTEIN, E AF GOFFMAN, TE RAUBITSCHEK, A MITCHELL, JB GLATSTEIN, E TI THE EMERGING BIOLOGY OF MODERN RADIATION ONCOLOGY SO CANCER RESEARCH LA English DT Review RP GOFFMAN, TE (reprint author), NCI,RADIAT ONCOL BRANCH,BLDG 10,ROOM B3-B69,BETHESDA,MD 20892, USA. NR 113 TC 14 Z9 14 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD DEC 15 PY 1990 VL 50 IS 24 BP 7735 EP 7744 PG 10 WC Oncology SC Oncology GA EM165 UT WOS:A1990EM16500001 PM 2253216 ER PT J AU PAI, LH FITZGERALD, DJ TEPPER, M SCHACTER, B SPITALNY, G PASTAN, I AF PAI, LH FITZGERALD, DJ TEPPER, M SCHACTER, B SPITALNY, G PASTAN, I TI INHIBITION OF ANTIBODY-RESPONSE TO PSEUDOMONAS EXOTOXIN AND AN IMMUNOTOXIN CONTAINING PSEUDOMONAS EXOTOXIN BY 15-DEOXYSPERGUALIN IN MICE SO CANCER RESEARCH LA English DT Article C1 NCI,MOLEC BIOL LAB,9000 ROCKVILLE PIKE,BLDG 37,ROOM 4E16,BETHESDA,MD 20892. BRISTOL MYERS SQUIBB CO,WALLINGFORD,CT 06942. NR 30 TC 59 Z9 61 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD DEC 15 PY 1990 VL 50 IS 24 BP 7750 EP 7753 PG 4 WC Oncology SC Oncology GA EM165 UT WOS:A1990EM16500003 PM 2253218 ER PT J AU SIEGALL, CB SCHWAB, G NORDAN, RP FITZGERALD, DJ PASTAN, I AF SIEGALL, CB SCHWAB, G NORDAN, RP FITZGERALD, DJ PASTAN, I TI EXPRESSION OF THE INTERLEUKIN-6 RECEPTOR AND INTERLEUKIN-6 IN PROSTATE CARCINOMA-CELLS SO CANCER RESEARCH LA English DT Article C1 NCI,DIV CANC TREATMENT,MED BRANCH,BETHESDA,MD 20892. RP SIEGALL, CB (reprint author), NCI,DIV CANC BIOL & DIAG,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 20 TC 152 Z9 156 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD DEC 15 PY 1990 VL 50 IS 24 BP 7786 EP 7788 PG 3 WC Oncology SC Oncology GA EM165 UT WOS:A1990EM16500010 PM 2253221 ER PT J AU MANN, VM DELAO, SL BRENES, M BRINTON, LA RAWLS, JA GREEN, M REEVES, WC RAWLS, WE AF MANN, VM DELAO, SL BRENES, M BRINTON, LA RAWLS, JA GREEN, M REEVES, WC RAWLS, WE TI OCCURRENCE OF IGA AND IGG ANTIBODIES TO SELECT PEPTIDES REPRESENTING HUMAN PAPILLOMAVIRUS TYPE-16 AMONG CERVICAL-CANCER CASES AND CONTROLS SO CANCER RESEARCH LA English DT Article C1 GORGAS MEM LAB,DIV EPIDEMIOL,PANAMA CITY,PANAMA. NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. ST LOUIS UNIV,INST MOLEC VIROL,ST LOUIS,MO 63110. CTR DIS CONTROL,DIV VIRAL & RICKETTSIAL DIS,VIRAL EXANTHAMS & HERPESVIRUSES BRANCH,ATLANTA,GA 30333. RP MANN, VM (reprint author), MCMASTER UNIV,DEPT PATHOL,MOLEC VIROL & IMMUNOL PROGRAM,HAMILTON L8N 3Z5,ONTARIO,CANADA. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 FU NCI NIH HHS [N01-CP-41026, R01-CA-42042] NR 24 TC 67 Z9 69 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD DEC 15 PY 1990 VL 50 IS 24 BP 7815 EP 7819 PG 5 WC Oncology SC Oncology GA EM165 UT WOS:A1990EM16500016 PM 2174733 ER PT J AU FORNACE, AJ PAPATHANASIOU, MA HOLLANDER, MC YAROSH, DB AF FORNACE, AJ PAPATHANASIOU, MA HOLLANDER, MC YAROSH, DB TI EXPRESSION OF THE O-6-METHYLGUANINE-DNA METHYLTRANSFERASE GENE MGMT IN MER+ AND MER- HUMAN TUMOR-CELLS SO CANCER RESEARCH LA English DT Article C1 APPL GENET INC,FREEPORT,NY 11520. RP FORNACE, AJ (reprint author), NCI,MOLEC PHARMACOL LAB,BLDG 37,ROOM 5C07,BETHESDA,MD 20892, USA. RI Fornace, Albert/A-7407-2008 OI Fornace, Albert/0000-0001-9695-085X NR 28 TC 77 Z9 78 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD DEC 15 PY 1990 VL 50 IS 24 BP 7908 EP 7911 PG 4 WC Oncology SC Oncology GA EM165 UT WOS:A1990EM16500032 PM 2253230 ER PT J AU BLANEY, SM BALIS, FM HEGEDUS, L HEIDEMAN, RL MCCULLY, C MURPHY, RF KELLEY, JA POPLACK, DG AF BLANEY, SM BALIS, FM HEGEDUS, L HEIDEMAN, RL MCCULLY, C MURPHY, RF KELLEY, JA POPLACK, DG TI PHARMACOKINETICS AND METABOLISM OF CYCLOPENTENYL CYTOSINE IN NONHUMAN-PRIMATES SO CANCER RESEARCH LA English DT Article C1 NCI,MED CHEM LAB,BETHESDA,MD 20892. WALTER REED ARMY MED CTR,WASHINGTON,DC 20307. RP BLANEY, SM (reprint author), NCI,PEDIAT BRANCH,BLDG 10,ROOM 13N240,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 25 TC 17 Z9 17 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD DEC 15 PY 1990 VL 50 IS 24 BP 7915 EP 7919 PG 5 WC Oncology SC Oncology GA EM165 UT WOS:A1990EM16500034 PM 2253232 ER PT J AU FUTAMI, H HORNUNG, RL BACK, TT BULL, R GRUYS, E WILTROUT, RH AF FUTAMI, H HORNUNG, RL BACK, TT BULL, R GRUYS, E WILTROUT, RH TI SYSTEMIC ALKALINIZATION INHIBITS THE ABILITY OF FLAVONE ACETIC-ACID TO AUGMENT NATURAL-KILLER ACTIVITY, INDUCE CYTOKINE GENE-EXPRESSION, AND SYNERGIZE WITH INTERLEUKIN-2 FOR THE TREATMENT OF MURINE RENAL-CANCER SO CANCER RESEARCH LA English DT Article C1 NCI,FREDERICK CANC RES FACIL,DIV CANC TREATMENT,EXPTL IMMUNOL LAB,FREDERICK,MD 21702. PRI DYN CORP,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21702. FU NCI NIH HHS [N01-CO-74102] NR 22 TC 10 Z9 10 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD DEC 15 PY 1990 VL 50 IS 24 BP 7926 EP 7931 PG 6 WC Oncology SC Oncology GA EM165 UT WOS:A1990EM16500036 PM 2253233 ER PT J AU CARDINALI, M UCHINO, R CHUNG, SI AF CARDINALI, M UCHINO, R CHUNG, SI TI INTERACTION OF FIBRINOGEN WITH MURINE MELANOMA-CELLS - COVALENT ASSOCIATION WITH CELL-MEMBRANES AND PROTECTION AGAINST RECOGNITION BY LYMPHOKINE-ACTIVATED KILLER-CELLS SO CANCER RESEARCH LA English DT Article C1 NIDR,CELLULAR DEV & ONCOL LAB,BLDG 30,ROOM 202,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 35 TC 38 Z9 38 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD DEC 15 PY 1990 VL 50 IS 24 BP 8010 EP 8016 PG 7 WC Oncology SC Oncology GA EM165 UT WOS:A1990EM16500050 PM 2253243 ER PT J AU JIA, WG BEAULIEU, C HUANG, FL CYNADER, MS AF JIA, WG BEAULIEU, C HUANG, FL CYNADER, MS TI PROTEIN-KINASE-C IMMUNOREACTIVITY IN KITTEN VISUAL-CORTEX IS DEVELOPMENTALLY REGULATED AND INPUT-DEPENDENT SO DEVELOPMENTAL BRAIN RESEARCH LA English DT Article DE POSTNATAL DEVELOPMENT; VISUAL CORTEX; PROTEIN KINASE-C; IMMUNOCYTOCHEMISTRY ID LONG-TERM POTENTIATION; POSTNATAL-DEVELOPMENT; RAT-BRAIN; SYNAPTIC PLASTICITY; GROWTH CONES; CAT; PHOSPHORYLATION; EXPRESSION; ISOZYMES; CELLS AB Immunocytochemistry with polyclonal antibodies directed against protein kinase C (PKC) was utilized to investigate the development of the kinase in kitten visual cortex neurons. The immunoreaction product was found at postsynaptic sites at all ages studied. However, PKC was localized in presynaptic terminals only during the first few weeks of postnatal life, during the period when the cortex is most susceptible to visual experience. The overall level of PKC immunoreactivity was high at early postnatal ages (up to 6 weeks) and declined afterwards till adulthood. This decline in reactivity was not equal across the cortex and was particularly marked in the middle cortical layers, especially layer IV. The reduction of PKC immunoreactivity in all cortical layers but layer IV was abolished by isolating a portion of cortex from its neuronal inputs early in life. Indirect evidence points to the lateral geniculate nucleus as the source of input that is required for input-dependent maturational changes in the kinase level. The results reported here suggest that the expression of PKC in kitten visual cortex in not only developmentally regulated but is also use-dependent. C1 NIH,BETHESDA,MD 20892. RP JIA, WG (reprint author), UNIV BRITISH COLUMBIA,DEPT OPHTHALMOL,VANCOUVER V5Z 3N9,BC,CANADA. NR 48 TC 25 Z9 25 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-3806 J9 DEV BRAIN RES JI Dev. Brain Res. PD DEC 15 PY 1990 VL 57 IS 2 BP 209 EP 221 DI 10.1016/0165-3806(90)90047-3 PG 13 WC Developmental Biology; Neurosciences SC Developmental Biology; Neurosciences & Neurology GA EN928 UT WOS:A1990EN92800007 ER PT J AU WISTOW, GJ PIATIGORSKY, J AF WISTOW, GJ PIATIGORSKY, J TI GENE CONVERSION AND SPLICE-SITE SLIPPAGE IN THE ARGININOSUCCINATE LYASES DELTA-CRYSTALLINS OF THE DUCK LENS - MEMBERS OF AN ENZYME SUPERFAMILY SO GENE LA English DT Article RP WISTOW, GJ (reprint author), NEI,MOLEC & DEV BIOL LAB,RM 204,BLDG 6,BETHESDA,MD 20892, USA. NR 49 TC 47 Z9 49 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 J9 GENE JI Gene PD DEC 15 PY 1990 VL 96 IS 2 BP 263 EP 270 DI 10.1016/0378-1119(90)90262-P PG 8 WC Genetics & Heredity SC Genetics & Heredity GA EM782 UT WOS:A1990EM78200016 PM 2269436 ER PT J AU PENDURTHI, TK PARKER, R SCHLOM, J PRIMUS, FJ AF PENDURTHI, TK PARKER, R SCHLOM, J PRIMUS, FJ TI LYMPHOKINE-ACTIVATED KILLER-CELL CYTOTOXICITY AGAINST HUMAN COLON CARCINOMAS ENHANCED BY MONOCLONAL ANTIBODY-D612 SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article C1 NCI,TUMOR IMMUNOL & BIOL LAB,9000 ROCKVILLE PIKE,BLDG 10,ROOM 8B07,BETHESDA,MD 20892. NR 46 TC 12 Z9 12 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD DEC 15 PY 1990 VL 46 IS 6 BP 1021 EP 1028 DI 10.1002/ijc.2910460613 PG 8 WC Oncology SC Oncology GA EN493 UT WOS:A1990EN49300011 PM 2123477 ER PT J AU MACKAY, AR HARTZLER, JL PELINA, MD THORGEIRSSON, UP AF MACKAY, AR HARTZLER, JL PELINA, MD THORGEIRSSON, UP TI STUDIES ON THE ABILITY OF 65-KDA AND 92-KDA TUMOR-CELL GELATINASES TO DEGRADE TYPE-IV COLLAGEN SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article C1 CATHOLIC UNIV AMER,WASHINGTON,DC 20064. RP MACKAY, AR (reprint author), NCI,DIV CANC ETIOL,OFF DIRECTOR,BETHESDA,MD 20892, USA. OI Mackay, Andrew Reay/0000-0001-7096-3759 NR 56 TC 110 Z9 110 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 15 PY 1990 VL 265 IS 35 BP 21929 EP 21934 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EM456 UT WOS:A1990EM45600083 PM 2174891 ER PT J AU BLITHE, DL AF BLITHE, DL TI N-LINKED OLIGOSACCHARIDES ON FREE-ALPHA INTERFERE WITH ITS ABILITY TO COMBINE WITH HUMAN CHORIONIC GONADOTROPIN-BETA SUBUNIT SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article RP BLITHE, DL (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892, USA. NR 36 TC 36 Z9 36 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 15 PY 1990 VL 265 IS 35 BP 21951 EP 21956 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EM456 UT WOS:A1990EM45600087 PM 1701438 ER PT J AU KIM, HC LEWIS, MS GORMAN, JJ PARK, SC GIRARD, JE FOLK, JE CHUNG, SI AF KIM, HC LEWIS, MS GORMAN, JJ PARK, SC GIRARD, JE FOLK, JE CHUNG, SI TI PROTRANSGLUTAMINASE-E FROM GUINEA-PIG SKIN - ISOLATION AND PARTIAL CHARACTERIZATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article C1 NIDR,CELLULAR DEV & ONCOL LAB,BLDG 30,RM 211,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NIH,NATL CTR RES RESOURCES,BETHESDA,MD 20892. SEOUL NATL UNIV,COLL MED,DEPT BIOCHEM,SEOUL 110,SOUTH KOREA. AMERICAN UNIV,DEPT CHEM,WASHINGTON,DC 20016. CSIRO,AUSTRALIAN ANIM HLTH LAB,GEELONG,VIC 3220,AUSTRALIA. NR 35 TC 108 Z9 110 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 15 PY 1990 VL 265 IS 35 BP 21971 EP 21978 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EM456 UT WOS:A1990EM45600090 PM 1979327 ER PT J AU MIERS, JB POSTLEWAITE, JC ZYUNG, TH CHEN, S ROEMIG, GR WEN, XN DLOTT, DD SZABO, A AF MIERS, JB POSTLEWAITE, JC ZYUNG, TH CHEN, S ROEMIG, GR WEN, XN DLOTT, DD SZABO, A TI DIFFUSION CAN EXPLAIN THE NONEXPONENTIAL REBINDING OF CARBON-MONOXIDE TO PROTOHEME SO JOURNAL OF CHEMICAL PHYSICS LA English DT Article ID SLOW CONFORMATIONAL-CHANGES; GEMINATE RECOMBINATION; HEME-PROTEINS; BINDING; PHOTOLYSIS; HEMOGLOBIN; MYOGLOBIN; DYNAMICS AB The recombination after flash photolysis of carbon monoxide (CO) to protoheme (PH) in glycerol: water is studied over ten decades in time (1 ps to 10 ms). The rebinding consists of an initial nonexponential geminate phase followed by a slower exponential bimolecular phase. The entire time course of this reaction between 260 and 300 K can be explained in a unified way using a simple, analytically tractable diffusion model involving just three parameters: the relative diffusion constant, the contact radius, and the intrinsic rate of reaction at contact. C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801. RI Szabo, Attila/H-3867-2012; OI Dlott, Dana/0000-0001-8719-7093 NR 28 TC 43 Z9 43 U1 1 U2 4 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0021-9606 J9 J CHEM PHYS JI J. Chem. Phys. PD DEC 15 PY 1990 VL 93 IS 12 BP 8771 EP 8776 DI 10.1063/1.459265 PG 6 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA EP160 UT WOS:A1990EP16000041 ER PT J AU TRAUB, RJ ALLEN, B HUMPHREY, E RUDA, MA AF TRAUB, RJ ALLEN, B HUMPHREY, E RUDA, MA TI ANALYSIS OF CALCITONIN GENE-RELATED PEPTIDE-LIKE IMMUNOREACTIVITY IN THE CAT DORSAL SPINAL-CORD AND DORSAL-ROOT GANGLIA PROVIDE EVIDENCE FOR A MULTISEGMENTAL PROJECTION OF NOCICEPTIVE C-FIBER PRIMARY AFFERENTS SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article C1 NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 30,RM 5-20,BETHESDA,MD 20892. OI Traub, Richard/0000-0001-8633-6311 NR 57 TC 61 Z9 61 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD DEC 15 PY 1990 VL 302 IS 3 BP 562 EP 574 DI 10.1002/cne.903020312 PG 13 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA EM094 UT WOS:A1990EM09400011 PM 1702117 ER PT J AU AMBRUS, JL CHESKY, L STEPHANY, D MCFARLAND, P MOSTOWSKI, H FAUCI, AS AF AMBRUS, JL CHESKY, L STEPHANY, D MCFARLAND, P MOSTOWSKI, H FAUCI, AS TI FUNCTIONAL-STUDIES EXAMINING THE SUBPOPULATION OF HUMAN LYMPHOCYTES-B RESPONDING TO HIGH-MOLECULAR-WEIGHT B-CELL GROWTH-FACTOR SO JOURNAL OF IMMUNOLOGY LA English DT Article ID NECROSIS FACTOR-ALPHA; NATURAL-KILLER CELLS; SURFACE IGD; RECOMBINANT INTERLEUKIN-4; DIFFERENTIATION; PROLIFERATION; EXPRESSION; RECEPTORS; ACTIVATION; ANTIBODY AB Mature human B lymphocytes perform many functions including antibody secretion, Ag presentation, preservation of memory for Ag, and lymphokine secretion. Individual resting B cells receive multiple sequential signals that determine the function(s) that will be performed by those cells. Activation signals such as Ag or Staphylococcus aureus Cowan I (Sac) stimulate overlapping but different subpopulations of B cells. After activation, B cells may be induced to proliferate by a variety of B cell growth factors (BCGF) including IL-2, IL-4, TNF-alpha, low molecular weight BCGF (HMW-BCGF), and high molecular weight BCGF (HMW-B CGF). Little information exists to explain why so many different BCGFs are involved with human B cell proliferation. The current studies were designed to examine the role HMW-BCGF plays in selecting B cells for particular functions. HMW-BCGF but not LMW-BCGF was found to inhibit Ig secretion when it was included in culture with Sac-activated B cells and B cell differentiation factors (BCDFs) including IL-6. Sorting resting B lymphocytes into surface IgD+ and IgD- populations and then stimulating each population with anti-mu revealed that the cells most responsive to HMW-BCGF resided in the surface IgD- sorted population. Sorting activated B lymphocytes into BA5 (HMW-BCGFR)+ and BA5- populations revealed that BA5+ B cells stimulated with BCDF (in the absence of HMW-BCGF) produced predominantly IgG, whereas the BA5- population produced both IgG and IgM. Finally, expansion of peripheral B cells from tetanus toxoid-immunized donors with either HMW-BCGF or LMW-BCGF revealed that the HMW-BCGF-expanded population produce d predominantly IgG tetanus-specific antibody in the presence of BCDF (in the absence of HMW-BCGF), whereas the LMW-BCGF-expanded population produced IgM >> IgG tetanus-specific antibody. Thus, HMW-BCGF may function to expand a subpopulation of B cells for memory B cell functions. C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NR 53 TC 24 Z9 25 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD DEC 15 PY 1990 VL 145 IS 12 BP 3949 EP 3955 PG 7 WC Immunology SC Immunology GA EP041 UT WOS:A1990EP04100001 PM 2258603 ER PT J AU ZACHARCHUK, CM MERCEP, M CHAKRABORTI, PK SIMONS, SS ASHWELL, JD AF ZACHARCHUK, CM MERCEP, M CHAKRABORTI, PK SIMONS, SS ASHWELL, JD TI PROGRAMMED LYMPHOCYTE-T DEATH - CELL ACTIVATION-INDUCED AND STEROID-INDUCED PATHWAYS ARE MUTUALLY ANTAGONISTIC SO JOURNAL OF IMMUNOLOGY LA English DT Article ID RECEPTOR COMPLEX; GLUCOCORTICOID RECEPTOR; MONOCLONAL-ANTIBODY; DNA FRAGMENTATION; ANTIGEN RECEPTOR; HORMONE RECEPTOR; CYCLE BLOCK; BINDING; APOPTOSIS; ENDONUCLEASE AB Both cellular activation signals and exposure to glucocorticoids such as dexamethasone (DEX) cause programmed cell death in T cell hybridomas. When cells were activated in the presence of Dex, however, the degree of killing that was achieved by either stimulus alone was markedly reduced. DEX-INDUCED PROGRAMMED CELL DEATH OF NORMAL T CELL CLONES WAS ALSO prevented by cellular activation. Cyclosporin A (CsA) completely blocked the activation-induced death of T cell hybridomas, but actually enhanced the killing caused by Dex. The addition of CsA to activated T cell hybridomas in the presence of Dex allowed killing to proceed, consistent with ability of CsA to block activation-induced nuclear gene transcription. A number of independent approaches were used to explore the effect of activation on the glucocorticoid signaling/effector pathway. First, RU-486, which binds the glucocorticoid receptor and is A POTENT COMPETITIVE ANTAGONIST OF DEX, DID NOT INHIBIT activation-induced cell killing. Second, activation of T cell hybridomas did not cause the translocation of the glucocorticoid receptor from the cytoplasm to the nucleus, nor did it prevent the receptor translocation induced by treatment with Dex. Finally, T cell hybridomas were transfected with a plasmid containing the chloramphenicol acetyltransferase (CAT) gene under the control of two tandemly arranged glucocorticoid-responsive elements. Activation of these cells did not induce CAT activity, and did not inhibit the CAT activity induced by Dex. In fact, there was a paradoxical in CAT activity when cells were treated with both stimuli. We conclude that cellular activation does not directly utilize the glucocorticoid receptor nor the glucocorticoid pathway when inducing programmed cell death. Furthermore, the ability of activation to inhibit Dex-mediated killing is not due to interference with the classical glucocorticoid signaling pathway, up to and including the initiation of gene transcription. Alternative mechanisms of antagonism, as well as the possible relevance of this phenomenon to the positive selection of self-recognizing thymocytes, are discussed. C1 NCI,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,BLDG 10,RM 13N-268,BETHESDA,MD 20892. NIDKD,ANALYT CHEM LAB,BETHESDA,MD 20892. NR 43 TC 314 Z9 317 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD DEC 15 PY 1990 VL 145 IS 12 BP 4037 EP 4045 PG 9 WC Immunology SC Immunology GA EP041 UT WOS:A1990EP04100013 PM 1979585 ER PT J AU PERCOPO, CM HOOKS, JJ SHINOHARA, T CASPI, R DETRICK, B AF PERCOPO, CM HOOKS, JJ SHINOHARA, T CASPI, R DETRICK, B TI CYTOKINE-MEDIATED ACTIVATION OF A NEURONAL RETINAL RESIDENT CELL PROVOKES ANTIGEN PRESENTATION SO JOURNAL OF IMMUNOLOGY LA English DT Article ID PIGMENT EPITHELIAL-CELLS; CLASS-II ANTIGENS; HLA-DR ANTIGEN; RETINITIS PIGMENTOSA; RENAL-ALLOGRAFTS; GRAVES-DISEASE; IA-ANTIGEN; EXPRESSION; RAT; MOLECULES AB The retinal pigment epithelial (RPE) cell has long been considered an important regulatory cell, maintaining physiological and structural balance within the retina. We have previously shown that the RPE cell may also be important in autoimmunity and transplantation. These cells can be induced by cytokines to express MHC class II Ag in ocular inflammatory and autoimmune conditions. In this report we show that isolated rat RPE cells can be induced to express class II Ag following incubation with rat rIFN-gamma. The ability of RPE cells to present Ag was determined by both T cell proliferation assays and IL-2 production. Only the Ia-positive RPE cells can present retinal Ag (S-Ag and interphotoreceptor-binding protein) to specifically sensitized rat Th cells. Moreover, the ability of chloroquine to inhibit this activity suggests that the RPE cell is also capable of processing Ag prior to Ag presentation. These studies indicate that cytokine-mediated activation of RPE cells may be a basic component of ocular immunity and an important aspect of RPE cell transplantation. C1 NEI,IMMUNOL & VIROL SECT,BLDG 10,ROOM 6N228,BETHESDA,MD 20892. OI Shinohara, Toshimichi/0000-0002-7197-9039 NR 40 TC 106 Z9 108 U1 0 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD DEC 15 PY 1990 VL 145 IS 12 BP 4101 EP 4107 PG 7 WC Immunology SC Immunology GA EP041 UT WOS:A1990EP04100022 PM 2147935 ER PT J AU BRISTOL, LA RUSCETTI, FW BRODY, DT DURUM, SK AF BRISTOL, LA RUSCETTI, FW BRODY, DT DURUM, SK TI IL-1-ALPHA INDUCES EXPRESSION OF ACTIVE TRANSFORMING GROWTH-FACTOR-BETA IN NONPROLIFERATING T-CELLS VIA A POSTTRANSCRIPTIONAL MECHANISM SO JOURNAL OF IMMUNOLOGY LA English DT Article ID COLONY-STIMULATING FACTOR; FACTOR-BETA; LYMPHOCYTES-T; INFLAMMATORY ARTHRITIS; MONOCLONAL-ANTIBODY; MESSENGER-RNA; CDNA CLONING; LATENT FORM; TGF-BETA; INTERLEUKIN-1 AB IL-1 is a co-stimulus for T cell proliferation along with TCR cross-linking agents or lectins. Here we demonstrate that IL-1-alpha, in the absence of a TCR stimulus, induces active TGF-beta production by non-proliferating T cells. Thus, in addition to being an incomplete signal, IL-1-alpha alone induced the production of a negative modulator for T cell proliferation. Interestingly, when Con A and IL-1 were combined, active TGF-beta was not detected in supernatants. Production of active TGF-beta protein by T cells in response to IL-1-alpha was established by 1) growth inhibition of the TGF-beta-responsive CCL 64 target cell, 2) neutralization of the activity in supernatants with an anti-TGF-beta mAb, and 3) competition of supernatants with [I-125] TGF-beta binding to CCL 64 membranes. The mechanism of IL-1-alpha induction of TGF-beta was examined: IL-1-alpha did not increase TGF-beta-1 message over constitutive levels, nor did it induce transcription of TGF-beta-2 message. A latent acid-activatable form of the protein was detected in nonproliferating murine T cells. We therefore suggest that IL-1-alpha regulates TGF-beta expression in T cells through translational and/or post-translational mechanisms. IL-1 regulation of active TGF-beta production by T cells may be a mechanism for controlling the proliferative response of T cells to a co-stimulus, and may be relevant in the pathophysiology of certain chronic inflammatory disease states. C1 NCI,MOLEC IMMUNOREGULAT LAB,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701. NR 36 TC 42 Z9 43 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD DEC 15 PY 1990 VL 145 IS 12 BP 4108 EP 4114 PG 7 WC Immunology SC Immunology GA EP041 UT WOS:A1990EP04100023 PM 2175324 ER PT J AU FUTAMI, H JANSEN, R MACPHEE, MJ KELLER, J MCCORMICK, K LONGO, DL OPPENHEIM, JJ RUSCETTI, FW WILTROUT, RH AF FUTAMI, H JANSEN, R MACPHEE, MJ KELLER, J MCCORMICK, K LONGO, DL OPPENHEIM, JJ RUSCETTI, FW WILTROUT, RH TI CHEMOPROTECTIVE EFFECTS OF RECOMBINANT HUMAN IL-1-ALPHA IN CYCLOPHOSPHAMIDE-TREATED NORMAL AND TUMOR-BEARING MICE - PROTECTION FROM ACUTE TOXICITY, HEMATOLOGIC EFFECTS, DEVELOPMENT OF LATE MORTALITY, AND ENHANCED THERAPEUTIC EFFICACY SO JOURNAL OF IMMUNOLOGY LA English DT Article ID COLONY-STIMULATING FACTOR; ENDOTHELIAL-CELLS; HUMAN INTERLEUKIN-1; INVIVO; IL-1; INDUCTION; RECOVERY; CANCER AB In this study, recombinant human IL-1-alpha (rhIL-1-alpha) was used to protect normal and tumor-bearing BALB/c mice from the acute toxicity caused by lethal doses of cyclophosphamide (Cy) and 5-fluorouracil. Pretreatment of mice for 7 days with 10,000 U/day of rhIL-1-alpha protected 70 to 100% of mice from the acute death induced by lethal doses of both Cy (380 mg/kg) and 5-fluorouracil (250 mg/kg). In contrast, post-treatment of mice with single or multiple doses of rhIL-1-alpha was not chemoprotective. Pretreatment of mice with rhIL-1-alpha increased the acute LD90of Cy from 380 mg/kg to > 500 mg/kg in normal mice, an LD90 dose-modifying effect of at least 1.25, was accompanied by a more rapid recovery from neutropenia and a less severe reduction in the number of bone marrow single lineage monocyte, myeloid, or myelomonocytic colonies. Some of the mice (10 to 50%) that were successfully protected by pretreatment with rhIL-1-alpha died after day 50. These mice consistently presented with extensive pulmonary inflammation and fibrosis at death. Mice bearing murine renal cancer (Renca) were also protected from the acute toxic effects of Cy (450 mg/kg) by pretreatment with rhIL-1-alpha. Renca-bearing mice pretreated with rhIL-1-alpha and either sublethal (300 mg/kg) or lethal (450 mg/kg) doses of Cy exhibited enhanced survival times over those of untreated Renca-bearing mice. Interestingly, the cause of death in Renca-bearing mice that ultimately failed treatment with rhIL-1-alpha plus 300 mg/kg Cy was recurrent tumor, whereas most mice treated with rhIL-1-alpha plus 450 mg/kg cy had no detectable tumor, although several died from late pulmonary inflammation and fibrosis. Thus, the dose escalation of Cy in rhIL-1-alpha-pretreated mice results in greater anti-tumor effects of Cy. However, the dose escalation of some cytotoxic agents allowed by the use of myelostimulatory agents can result in late fatal complications not detected in acute toxicity testing. C1 NCI,FREDERICK CANC RES FACIL,EXPTL IMMUNOL LAB,BLDG 560,ROOM 31-93,FREDERICK,MD 21701. NCI,FREDERICK CANC RES FACIL,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21701. NCI,FREDERICK CANC RES FACIL,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701. NCI,FREDERICK CANC RES FACIL,PROGRAM RESOURCES INC,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21701. NR 31 TC 44 Z9 44 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD DEC 15 PY 1990 VL 145 IS 12 BP 4121 EP 4130 PG 10 WC Immunology SC Immunology GA EP041 UT WOS:A1990EP04100025 PM 2258610 ER PT J AU VARESIO, L CLAYTON, M BLASI, E RUFFMAN, R RADZIOCH, D AF VARESIO, L CLAYTON, M BLASI, E RUFFMAN, R RADZIOCH, D TI PICOLINIC-ACID, A CATABOLITE OF TRYPTOPHAN, AS THE 2ND SIGNAL IN THE ACTIVATION OF IFN-GAMMA-PRIMED MACROPHAGES SO JOURNAL OF IMMUNOLOGY LA English DT Article ID MURINE PERITONEAL-MACROPHAGES; HUMAN-CELLS INVITRO; INTERFERON-GAMMA; RIBOSOMAL-RNA; PROTEIN-SYNTHESIS; INDOLEAMINE 2,3-DIOXYGENASE; MOUSE MACROPHAGES; BETA; EXPRESSION; INVIVO AB We have studied the effects of picolinic acid, a product of tryptophan degradation, on the activation of mouse peritoneal macrophages (M-phi). Picolinic acid acts synergistically with IFN-gamma in activating M-phi from C57BL/6 mice. Moreover, M-phi from C3H/HeJ mice and C3H/HeN that do not become cytotoxic in response to IFN-gamma alone could be fully activated by exposure to picolinate plus IFN-gamma. These results indicate that picolinic acid is a potent costimulator of M-phi activation that functions as a second signal. Inasmuch as we have previously demonstrated that the activation of cytotoxic M-phi correlated with specific changes in ribosomal RNA (rRNA), we investigated whether picolinic acid could modify M-phi RNA metabolism. Picolinic acid inhibited the synthesis of total M-phi RNA, the accumulation of newly synthesized 28S rRNA, and augmented the steady state levels of rRNA precursors (pre-rRNA). These changes in RNA metabolism were similar to those previously described in murine M-phi activated in vitro or in vivo to express tumoricidal activity. These results demonstrate that picolinic acid is a potent, biologic M-phi second signal, suggest that the changes in rRNA are causally connected with the expression of tumoricidal activity, and suggest the existance of an autocrine effect mediated by picolinic acid. C1 UNIV PERUGIA,INST MICROBIOL,I-06100 PERUGIA,ITALY. RECORDATI,DEPT INT MED,MILAN,ITALY. MCGILL UNIV,MONTREAL GEN HOSP,DEPT MED,MONTREAL H3G 1A4,QUEBEC,CANADA. RP VARESIO, L (reprint author), NCI,DIV CANC TREATMENT,IMMUNOBIOL SECT,MOLEC IMMUNOREGULAT LAB,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701, USA. RI varesio, luigi/J-8261-2016; Blasi, Elisabetta/F-5870-2015 OI varesio, luigi/0000-0001-5659-2218; Blasi, Elisabetta/0000-0002-4202-8293 NR 44 TC 58 Z9 58 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD DEC 15 PY 1990 VL 145 IS 12 BP 4265 EP 4271 PG 7 WC Immunology SC Immunology GA EP041 UT WOS:A1990EP04100044 PM 1701787 ER PT J AU PUENTES, SM DASILVA, RP SACKS, DL HAMMER, CH JOINER, KA AF PUENTES, SM DASILVA, RP SACKS, DL HAMMER, CH JOINER, KA TI SERUM RESISTANCE OF METACYCLIC STAGE LEISHMANIA-MAJOR PROMASTIGOTES IS DUE TO RELEASE OF C5B-9 SO JOURNAL OF IMMUNOLOGY LA English DT Article ID ALTERNATIVE COMPLEMENT PATHWAY; CYCLE-DEPENDENT GENERATION; TRYPANOSOMA-CRUZI; POLYMORPHONUCLEAR LEUKOCYTES; DEVELOPMENTAL STAGES; INFECTIVE STAGE; MEMBRANE; EXPRESSION; BINDING; ATTACK AB The mechanism of serum resistance for infective promastigotes of Leishmania major was investigated. Prior results suggested that the mechanism of resistance was mediated at a step after C3 deposition. Equivalent amounts of C3b were deposited on serum-susceptible, noninfective promastigotes harvested from log stage cultures (LOG) and on C-resistant, infective, metacyclic promastigotes (MP) purified from stationary stage cultures. Whereas binding of C9 to LOG was stable during incubation in serum, C9 binding to MP was minimal and unstable, because molecules bound initially to MP were released with continued incubation. Failure to bind C9 was not a result of inability to activate C; the kinetics of C3, C6, and C9 consumption were similar for LOG and MP. Deposition of C5b-7 on MP was stable, indicating that the initial steps in terminal complex formation were intact. Instead, the majority of C5b-9 formed on MP was spontaneously released into the serum as SC5b-9. Residual C5b-9 on MP was released with 1 M NaCl. These data show that developmental modification of the promastigote membrane during transition from a noninfective to an infective stage blocks insertion of lytic C5b-9 into the promastigote membrane. C1 NIAID,PARASIT DIS LAB,BLDG 4,ROOM 126,BETHESDA,MD 20892. NIAID,CLIN INVEST LAB,BETHESDA,MD 20892. NR 36 TC 108 Z9 110 U1 1 U2 6 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD DEC 15 PY 1990 VL 145 IS 12 BP 4311 EP 4316 PG 6 WC Immunology SC Immunology GA EP041 UT WOS:A1990EP04100051 PM 2147941 ER PT J AU SHIP, JA BAUM, BJ AF SHIP, JA BAUM, BJ TI IS REDUCED SALIVARY FLOW NORMAL IN OLD-PEOPLE SO LANCET LA English DT Letter RP SHIP, JA (reprint author), NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892, USA. NR 10 TC 59 Z9 62 U1 1 U2 4 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD DEC 15 PY 1990 VL 336 IS 8729 BP 1507 EP 1507 DI 10.1016/0140-6736(90)93212-8 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EN157 UT WOS:A1990EN15700033 PM 1979113 ER PT J AU BUCK, MA FRASER, CM AF BUCK, MA FRASER, CM TI MUSCARINIC ACETYLCHOLINE-RECEPTOR SUBTYPES WHICH SELECTIVELY COUPLE TO PHOSPHOLIPASE-C - PHARMACOLOGICAL AND BIOCHEMICAL-PROPERTIES SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article C1 NIAAA,PHYSIOL & PHARMACOL STUDIES,MOLEC NEUROBIOL SECT,ROCKVILLE,MD 20852. OI Fraser, Claire/0000-0003-1462-2428 NR 23 TC 41 Z9 42 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD DEC 14 PY 1990 VL 173 IS 2 BP 666 EP 672 DI 10.1016/S0006-291X(05)80087-7 PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EN310 UT WOS:A1990EN31000029 PM 2175603 ER PT J AU GREIG, NH STAHLE, PL SHETTY, HU GENKA, S JOHN, V SONCRANT, TT RAPOPORT, SI AF GREIG, NH STAHLE, PL SHETTY, HU GENKA, S JOHN, V SONCRANT, TT RAPOPORT, SI TI HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF CHLORAMBUCIL TERT-BUTYL ESTER AND ITS ACTIVE METABOLITES CHLORAMBUCIL AND PHENYLACETIC MUSTARD IN PLASMA AND TISSUE SO JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS LA English DT Note ID ACETIC MUSTARD; PHARMACOKINETICS; ASSAY; PREDNIMUSTINE C1 ATHENA NEUROSCI INC,S SAN FRANCISCO,CA 94080. RP GREIG, NH (reprint author), NIA,NEUROSCI LAB,BETHESDA,MD 20892, USA. NR 14 TC 9 Z9 9 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4347 J9 J CHROMATOGR-BIOMED JI J. Chromatogr.-Biomed. Appl. PD DEC 14 PY 1990 VL 534 BP 279 EP 286 DI 10.1016/S0378-4347(00)82175-1 PG 8 WC Chemistry, Analytical SC Chemistry GA ET068 UT WOS:A1990ET06800033 PM 2094719 ER PT J AU ZHOU, HX AF ZHOU, HX TI KINETICS OF DIFFUSION-INFLUENCED REACTIONS STUDIED BY BROWNIAN DYNAMICS SO JOURNAL OF PHYSICAL CHEMISTRY LA English DT Article RP ZHOU, HX (reprint author), NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892, USA. RI Zhou, Huan-Xiang/M-5170-2016 OI Zhou, Huan-Xiang/0000-0001-9020-0302 NR 36 TC 53 Z9 53 U1 0 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-3654 J9 J PHYS CHEM-US JI J. Phys. Chem. PD DEC 13 PY 1990 VL 94 IS 25 BP 8794 EP 8800 DI 10.1021/j100388a010 PG 7 WC Chemistry, Physical SC Chemistry GA EM664 UT WOS:A1990EM66400010 ER PT J AU LEVY, D GARRISON, RJ KANNEL, WB CASTELLI, WP AF LEVY, D GARRISON, RJ KANNEL, WB CASTELLI, WP TI PROGNOSTIC IMPLICATIONS OF ECHOCARDIOGRAPHICALLY DETERMINED LEFT-VENTRICULAR MASS IN THE FRAMINGHAM HEART-STUDY - REPLY SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 NHLBI,BETHESDA,MD 20892. BOSTON UNIV,SCH MED,BOSTON,MA 02118. RP LEVY, D (reprint author), FRAMINGHAM HEART DIS EPIDEMIOL STUDY,FRAMINGHAM,MA 01701, USA. NR 6 TC 17 Z9 18 U1 1 U2 6 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD DEC 13 PY 1990 VL 323 IS 24 BP 1706 EP 1707 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA EL856 UT WOS:A1990EL85600016 ER PT J AU WATSON, JD AF WATSON, JD TI FUNDING THE HUMAN-GENOME-PROJECT - REPLY SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter RP WATSON, JD (reprint author), NIH,NATL CTR HUMAN GENOME RES,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 12 PY 1990 VL 264 IS 22 BP 2867 EP 2867 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EL847 UT WOS:A1990EL84700006 ER PT J AU PAN, CJ HOEPPNER, W CHOU, JY AF PAN, CJ HOEPPNER, W CHOU, JY TI INDUCTION OF PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE-EXPRESSION BY RETINOIC ACID IN AN ADULT-RAT HEPATOCYTE LINE SO BIOCHEMISTRY LA English DT Article C1 NICHHD,HUMAN GENET BRANCH,BLDG 10,ROOM 9S242,BETHESDA,MD 20892. UNIV HAMBURG,KRANKENHAUS EPPENDORF,INST PHYSIOL CHEM,W-2000 HAMBURG 20,GERMANY. NR 41 TC 37 Z9 37 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD DEC 11 PY 1990 VL 29 IS 49 BP 10883 EP 10888 DI 10.1021/bi00501a004 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EM296 UT WOS:A1990EM29600004 PM 2176887 ER PT J AU GLAUDEMANS, CPJ LERNER, L DAVES, GD KOVAC, P VENABLE, R BAX, A AF GLAUDEMANS, CPJ LERNER, L DAVES, GD KOVAC, P VENABLE, R BAX, A TI SIGNIFICANT CONFORMATIONAL-CHANGES IN AN ANTIGENIC CARBOHYDRATE EPITOPE UPON BINDING TO A MONOCLONAL-ANTIBODY SO BIOCHEMISTRY LA English DT Article C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. US FDA,CTR BIOL EVALUAT & RES,BETHESDA,MD 20892. RP GLAUDEMANS, CPJ (reprint author), NIDDKD,CHEM LAB,BETHESDA,MD 20892, USA. NR 50 TC 61 Z9 61 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD DEC 11 PY 1990 VL 29 IS 49 BP 10906 EP 10911 DI 10.1021/bi00501a007 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EM296 UT WOS:A1990EM29600007 PM 1703008 ER PT J AU JOHNSTON, CA LOPEZ, F SAMSON, WK NEGROVILAR, A AF JOHNSTON, CA LOPEZ, F SAMSON, WK NEGROVILAR, A TI PHYSIOLOGICALLY IMPORTANT ROLE FOR CENTRAL OXYTOCIN IN THE PREOVULATORY RELEASE OF LUTEINIZING-HORMONE SO NEUROSCIENCE LETTERS LA English DT Article DE OXYTOCIN; LUTEINIZING HORMONE; PROESTRUS; PREOVULATORY; PASSIVE IMMUNIZATION; CENTRAL NERVOUS SYSTEM ID PROLACTIN SECRETION; NEUROHYPOPHYSEAL HORMONES; RAT; PROESTRUS AB Recently, our laboratory has provided evidence for a physiologically relevant stimulatory influence of oxytocin (OXY) on the preovulatory luteinizing hormone (LH) surge in cycling female rats. The present study evaluated whether this stimulatory effect of OXY on LH release is exerted at a central or peripheral site of action by comparing the ability of peripheral (intravenous) or central (intracerebroventricular) administration of OXY antisera to influence the preovulatory LH surge. The peripheral injection of a very large dose of OXY antisera (0.8 ml) caused a slight attenuation of the early stages of the LH surge. In contrast, the central administration of 5 mu-l of OXY antisera completely abolished the preovulatory LH surge. The data support the hypothesis that OXY exerts a physiologically important stimulatory influence on the preovulatory LH surge which is mediated primarily at a central site of action. C1 WASHINGTON STATE UNIV,COLL PHARM,PULLMAN,WA 99164. NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,REPROD NEUROENDOCRINOL SECT,RES TRIANGLE PK,NC 27709. UNIV MISSOURI,SCH MED,DEPT ANAT,COLUMBIA,MO 65212. NR 14 TC 34 Z9 34 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD DEC 11 PY 1990 VL 120 IS 2 BP 256 EP 258 DI 10.1016/0304-3940(90)90053-C PG 3 WC Neurosciences SC Neurosciences & Neurology GA EP143 UT WOS:A1990EP14300031 PM 2293113 ER PT J AU BERKHOUT, B JEANG, KT AF BERKHOUT, B JEANG, KT TI DOWN MODULATION OF HIV-1 GENE-EXPRESSION USING A PROKARYOTIC RNA-BINDING PROTEIN SO NUCLEIC ACIDS RESEARCH LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS; R17 COAT PROTEIN; TRANS-ACTIVATION; MESSENGER-RNA; TAT PROTEIN; NUCLEOTIDE-SEQUENCE; SYNTHETIC VARIANTS; MAMMALIAN-CELLS; EARLY PROMOTER; LOOP SEQUENCE AB The coat protein of the single stranded RNA bacteriophages acts as a translational repressor by binding with high affinity to a target RNA that encompasses the ribosomal binding site of the replicase gene. We have expressed this procaryotic RNA-binding protein in mammalian cells. Using the coat protein binding site attached to the HIV-1 5' leader RNA, we tested for the biological effect of co-expressed bacteriophage protein. We found that HIV-1 LTR-directed expression within this context was inhibited in trans by the coat protein. This example suggests the feasibility of using procaryotic RNa-binding proteins as genetic modulators in eucaryotic cells. RP BERKHOUT, B (reprint author), NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892, USA. RI Jeang, Kuan-Teh/A-2424-2008 NR 44 TC 6 Z9 6 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 11 PY 1990 VL 18 IS 23 BP 6903 EP 6907 DI 10.1093/nar/18.23.6903 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EP140 UT WOS:A1990EP14000022 PM 2124673 ER PT J AU AREPALLI, SR HELLER, M GLAUDEMANS, CPJ AF AREPALLI, SR HELLER, M GLAUDEMANS, CPJ TI SEQUENCE OF THE V(H) GENE FOR MURINE IGA X-24 SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 NCI,GENET LAB,BETHESDA,MD 20892. RP AREPALLI, SR (reprint author), NIDDK,CHEM LAB,BETHESDA,MD 20892, USA. NR 7 TC 2 Z9 2 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 11 PY 1990 VL 18 IS 23 BP 7152 EP 7152 DI 10.1093/nar/18.23.7152 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EP140 UT WOS:A1990EP14000072 PM 2124679 ER PT J AU KENNEY, RT LETO, TL AF KENNEY, RT LETO, TL TI A HINDIII POLYMORPHISM IN THE HUMAN NCF2 GENE SO NUCLEIC ACIDS RESEARCH LA English DT Note RP KENNEY, RT (reprint author), NIAID,CLIN INVEST LAB,BLDG 10,ROOM 11N110,BETHESDA,MD 20892, USA. NR 2 TC 6 Z9 6 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 11 PY 1990 VL 18 IS 23 BP 7193 EP 7193 DI 10.1093/nar/18.23.7193-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EP140 UT WOS:A1990EP14000111 PM 1979859 ER PT J AU POLYMEROPOULOS, MH RATH, DS XIAO, H MERRIL, CR AF POLYMEROPOULOS, MH RATH, DS XIAO, H MERRIL, CR TI TRINUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN INTESTINAL FATTY-ACID BINDING-PROTEIN GENE (FABP2) SO NUCLEIC ACIDS RESEARCH LA English DT Note RP POLYMEROPOULOS, MH (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,ROOM 131,2700 MARTIN LUTHER KING AVE,WASHINGTON,DC 20032, USA. NR 3 TC 27 Z9 27 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 11 PY 1990 VL 18 IS 23 BP 7198 EP 7198 DI 10.1093/nar/18.23.7198-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EP140 UT WOS:A1990EP14000121 PM 2263509 ER PT J AU DEAN, M STEWART, C PERRY, A STAUFFER, D OTTERUD, B WHITE, R LEPPERT, M AF DEAN, M STEWART, C PERRY, A STAUFFER, D OTTERUD, B WHITE, R LEPPERT, M TI D7S449 DETECTS A HINDIII POLYMORPHISM TIGHTLY LINKED TO THE MET GENE ON CHROMOSOME-7 SO NUCLEIC ACIDS RESEARCH LA English DT Note C1 UNIV UTAH,HOWARD HUGHES MED INST,SALT LAKE CITY,UT 84132. UNIV UTAH,DEPT HUMAN GENET,SALT LAKE CITY,UT 84132. RP DEAN, M (reprint author), PROGRAM RESOURCES INC,DYNCORP,FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21702, USA. RI Dean, Michael/G-8172-2012 OI Dean, Michael/0000-0003-2234-0631 FU NCI NIH HHS [N01-CO-74102] NR 2 TC 2 Z9 2 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 11 PY 1990 VL 18 IS 23 BP 7199 EP 7199 DI 10.1093/nar/18.23.7199-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EP140 UT WOS:A1990EP14000123 PM 1979868 ER PT J AU AMBUDKAR, IS HORN, VJ DAI, YS BAUM, BJ AF AMBUDKAR, IS HORN, VJ DAI, YS BAUM, BJ TI EVIDENCE AGAINST A ROLE FOR A PERTUSSIS TOXIN-SENSITIVE G-PROTEIN IN CA-2+ MOBILIZATION IN RAT PAROTID ACINAR-CELLS SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Article DE PERTUSSIS TOXIN; G-PROTEIN; CALCIUM ION MOBILIZATION; SIGNAL TRANSDUCTION; PAROTID CELL; (RAT) ID NUCLEOTIDE-BINDING-PROTEINS; GUANINE-NUCLEOTIDE; PHOSPHOLIPASE-C; POLYPHOSPHOINOSITIDE PHOSPHODIESTERASE; INOSITOL 1,4,5-TRISPHOSPHATE; ADRENERGIC-RECEPTOR; MUSCARINIC-RECEPTOR; ADENYLATE-CYCLASE; CALCIUM CHANNELS; ACTIVATION AB Hormone-induced Ca2+ mobilization in rat parotid acinar cells is reportedly mediated via an as yet uncharacterized G protein. We have studied the sensitivity to pertussis toxin (PTx) of this signal transduction mechanism. When rats were treated with Ptx (1.3-1.5-mu-g per animal) for 72 h, a 41 kDa membrane protein was ADP-ribosylated. This PTx treatment regimen, also, resulted in a more than 80% block of the ability of the muscarinic agonist carbachol to inhibit beta-adrenergic receptor-stimulated parotid adenylyl cyclase activity. However, cytosolic Ca2+ levels, in response to either carbachol or AIF4-, were comparable in cells prepared from both untreated or PTx-treated rats, when incubated either in the absence or presence of extracellular Ca2+. Further, both the sensitivity of the Ca2+ response to carbachol and the ability of the agonist-sensitive intracellular Ca2+ stores to be refilled by extracellular Ca2+ were unaffected by PTx treatment. Parotid membranes also contained three low-molecular-weight GTP-binding proteins (25, 22 and 18 kDa) which were unaffected by PTx. These results show that there is only one detectable substrate in parotid membranes for a PTx-catalyzed ADP-ribosylation and that hormone-induced Ca2+ mobilization events in parotid acinar cells are not mediated via PTx-sensitive components. C1 NIDR,CLIN INVEST & PATIENT CARE BRANCH,BLDG 10,ROOM 1N-113,BETHESDA,MD 20892. NR 44 TC 11 Z9 11 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD DEC 10 PY 1990 VL 1055 IS 3 BP 259 EP 264 DI 10.1016/0167-4889(90)90041-B PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EP507 UT WOS:A1990EP50700009 PM 2124929 ER PT J AU FLORENTINO, A VARGA, K KUNOS, G AF FLORENTINO, A VARGA, K KUNOS, G TI MECHANISM OF THE CARDIOVASCULAR EFFECTS OF GABA(B) RECEPTOR ACTIVATION IN THE NUCLEUS TRACTUS SOLITARII OF THE RAT SO BRAIN RESEARCH LA English DT Article DE BACLOFEN; 2-HYDROXY-SACLOFEN; PHACLOFEN; GLUTAMATE; BAROREFLEX ID GAMMA-AMINOBUTYRIC ACID; BLOOD-PRESSURE; BACLOFEN; INHIBITION; GLUTAMATE; BAROREFLEX; RESPONSES; PHACLOFEN; REFLEXES; MEDULLA AB The effects of baclofen microinjected into the nucleus tractus solitarii (NTS) on blood pressure, heart rate and baroreflex bradycardia were studied in urethane-anesthetized rats. Baclofen caused dose-dependent pressor and tachycardic effects and inhibited the reflex bradycardia elicited by i.v. phenylephrine. The effects of baclofen were inhibited by similarly administered GABA(B) receptor antagonists, phaclofen and 2-OH-saclofen, or the non-NMDA glutamate receptor antagonist, DNQX, or by pretreatment of rats with intracisternally administered pertussis toxin. DNQX and pertussis toxin, but not the NMDA antagonist, MK-801, also inhibited baroreflex bradycardia. Intra-NTS injections of glutamate caused hypotension and bradycardia, which were potentiated by baclofen, and were not affected by either DNQX or MK-801, or by pretreatment with pertussis toxin. These findings indicate that the cardiovascular effects of stimulation of GABA(B) receptors in the NTS are due, at least in part, to inhibition of the depressor baroreflex response. Inhibition of the release and/or postsynaptic action of an excitatory amino acid transmitter other than glutamate is the most likely mechanism. C1 NIAAA,PHYSIOL & PHARMACOL STUDIES LAB,12501 WASHINGTON AVE,ROCKVILLE,MD 20852. NR 33 TC 51 Z9 51 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD DEC 10 PY 1990 VL 535 IS 2 BP 264 EP 270 DI 10.1016/0006-8993(90)91609-K PG 7 WC Neurosciences SC Neurosciences & Neurology GA EN903 UT WOS:A1990EN90300011 PM 1963570 ER PT J AU DUBNER, R AF DUBNER, R TI THE NEURAL BASIS OF ORAL-FACIAL FUNCTION AND PAIN - A CITATION-CLASSIC COMMENTARY ON THE NEURAL BASIS OF ORAL AND FACIAL FUNCTION BY DUBNER,R., SESSLE,B.J., AND STOREY,A.T. SO CURRENT CONTENTS/SOCIAL & BEHAVIORAL SCIENCES LA English DT Article RP DUBNER, R (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU INST SCI INFORM INC PI PHILADELPHIA PA 3501 MARKET ST, PHILADELPHIA, PA 19104 SN 0092-6361 J9 CC/SOC BEHAV SCI PD DEC 10 PY 1990 IS 50 BP 18 EP 18 PG 1 WC Multidisciplinary Sciences; Social Sciences, Interdisciplinary SC Science & Technology - Other Topics; Social Sciences - Other Topics GA EK531 UT WOS:A1990EK53100001 ER PT J AU STEMMER, P KLEE, CB AF STEMMER, P KLEE, CB TI ELECTROSTATIC REPULSION BETWEEN MOLECULES OF LIKE CHARGE CAN BE MISINTERPRETED AS BINDING SO FEBS LETTERS LA English DT Article DE EDTA BINDING; ELECTROSTATIC REPULSION; CALMODULIN; ALPHA-LACTALBUMIN; CHELATOR ID ALPHA-LACTALBUMIN; CALCIUM-BINDING; CALMODULIN; ACID AB Spectroscopic methods have shown that Ca2+ chelators interact with Ca2+ -binding proteins. These spectral alterations have been interpreted as evidence for the binding of chelator by the proteins. We show by direct examination of EDTA interaction with calmodulin and alpha-lactalbumin that these proteins repel EDTA rather than bind it. The repulsion is reduced by increased salt concentration but is unaffected by Ca2+ binding to the proteins. The acidic protein, alpha-lactalbumin, repells that negatively charged EDTA and inorganic phosphate whereas the basic protein, lysozyme, repells the positively charged spermine. Thus, spectroscopic changes induced by negatively charged Ca2+ chelators on negatively charged Ca2+ -binding proteins are due to electrostatic repulsion, and not to binding. These observations underscore the possible pitfalls of using spectroscopic methods alone to analyze protein-ligand interactions. C1 NCI,BIOCHEM LAB,BLDG 37,RM 4E 28,BETHESDA,MD 20892. NR 15 TC 4 Z9 4 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD DEC 10 PY 1990 VL 276 IS 1-2 BP 71 EP 74 DI 10.1016/0014-5793(90)80509-H PG 4 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA EP342 UT WOS:A1990EP34200018 PM 2125011 ER PT J AU WENTHOLD, RJ HUNTER, C WADA, K DECHESNE, CJ AF WENTHOLD, RJ HUNTER, C WADA, K DECHESNE, CJ TI ANTIBODIES TO A C-TERMINAL PEPTIDE OF THE RAT-BRAIN GLUTAMATE RECEPTOR SUBUNIT, GLUR-A, RECOGNIZE A SUBPOPULATION OF AMPA BINDING-SITES BUT NOT KAINATE SITES SO FEBS LETTERS LA English DT Article DE EXCITATORY AMINO ACID RECEPTOR; RECEPTOR IMMUNOPRECIPITATION; GLUTAMATE; NEUROTRANSMITTER ID XENOPUS OOCYTES; ACID; QUISQUALATE; PROTEINS; EXPRESSION; MEMBRANES; RESPONSES; LIGAND AB Antibodies were made to a thirteen amino acid synthetic peptide corresponding to the C-terminal portion of the glutamate (glu) receptor, GluR-A. The immunoprecipitation of kainic acid (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) binding sites by the anti-peptide antibodies was studied using a detergent-solubilized preparation of rat brain membranes. Under these conditions a subpopulation of AMPA binding sites was recognized by the antibodies, but no KA binding sites were recognized. Scatchard analysis of this subpopulation of AMPA binding sites yields a curvilinear plot which fits a two-site model with dissociation constants of 4.6 and 323 nM. These studies show that the glu receptor complex, GluR-A, binds AMPA but not KA and suggest that (i) the binding sites for these two ligands reside on different proteins, and (ii) the KA receptor identified physiologically is not equivalent to the KA binding sites identified with H-3-labelled KA. RP WENTHOLD, RJ (reprint author), NIDOCD,NEUROCHEM SECT,MOLEC OTOL LAB,BLDG 36,ROOM 5D08,BETHESDA,MD 20892, USA. NR 27 TC 41 Z9 41 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD DEC 10 PY 1990 VL 276 IS 1-2 BP 147 EP 150 DI 10.1016/0014-5793(90)80529-R PG 4 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA EP342 UT WOS:A1990EP34200038 PM 2176160 ER PT J AU KHANUM, A DUFAU, ML AF KHANUM, A DUFAU, ML TI A CAMP INDEPENDENT INHIBITORY-ACTION OF HIGH-DOSES OF FORSKOLIN IN RAT LEYDIG-CELLS SO JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article ID NUCLEOTIDE REGULATORY PROTEIN; AMP-GENERATING SYSTEMS; CYCLIC-AMP; ADENYLATE-CYCLASE; DITERPENE FORSKOLIN; LUTEINIZING-HORMONE; GONADOTROPIN; ACTIVATION; STEROIDOGENESIS; ACCUMULATION AB In addition to well known direct stimulatory and potentiatory actions of forskolin, we have previously reported that low doses of this diterpene (10(-9), 10(-12) M) markedly inhibit the production of cAMP and testosterone in rat Leydig cells through a pertussis toxin sensitive G-protein (A. Khanum and M. L. Dufau, J. Biol. Chem. 261, 1986). A different type of inhibitory effect of forskolin is described in this study. Forskolin (10(-5) M) markedly stimulates basal adenylate cyclase activity (about 200%) in rat Leydig cell membranes and potentiates the stimulatory effect of gonadotropin (10(-9), 10(-7) M) on adenylate cyclase in presence or in absence of GTP (10(-9), 10(-7) M) on adenylate cyclase in presence or in absence of GTP (10(-5) M). Similarly a time-dependent stimulation of forskolin (10(-5) M) alone is noted on all cAMP pools and testosterone production. Using a supramaximal steroidogenic dose of hCG (0.26 nM) or choleragen (0.1-mu-M), forskolin potentiates the gonadotrophin and toxin-induced responses of all cAMP pools significantly while inhibiting testosterone production. Moreover, forskolin also inhibits 8-Bromo-cAMP stimulated steroidogenesis. In contrast, pregnenolone synthesis was not altered by the diterpene. We have demonstrated in this study that the inhibitory effect of high doses of forskolin on steroidogenesis is distal to cAMP generation, and resulted from a steroidogenic block residing beyond pregnenolone synthesis. C1 NICHHD,ENDOCRINOL & REPROD RES BRANCH,MOLEC ENDOCRINOL SECT,BETHESDA,MD 20892. NR 29 TC 8 Z9 8 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0960-0760 J9 J STEROID BIOCHEM JI J. Steroid Biochem. Mol. Biol. PD DEC 10 PY 1990 VL 37 IS 5 BP 669 EP 674 DI 10.1016/0960-0760(90)90349-P PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA EV477 UT WOS:A1990EV47700006 PM 2177627 ER PT J AU CHEN, ZQ BURDETT, LA SETH, AK LAUTENBERGER, JA PAPAS, TS AF CHEN, ZQ BURDETT, LA SETH, AK LAUTENBERGER, JA PAPAS, TS TI REQUIREMENT OF ETS-2 EXPRESSION FOR XENOPUS OOCYTE MATURATION SO SCIENCE LA English DT Article C1 NCI,MOLEC ONCOL LAB,FREDERICK,MD 21701. NR 32 TC 42 Z9 42 U1 0 U2 1 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD DEC 7 PY 1990 VL 250 IS 4986 BP 1416 EP 1418 DI 10.1126/science.2255913 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EL843 UT WOS:A1990EL84300045 PM 2255913 ER PT J AU APLAN, PD LOMBARDI, DP GINSBERG, AM COSSMAN, J BERTNESS, VL KIRSCH, IR AF APLAN, PD LOMBARDI, DP GINSBERG, AM COSSMAN, J BERTNESS, VL KIRSCH, IR TI DISRUPTION OF THE HUMAN SCL LOCUS BY ILLEGITIMATE V-(D)-J RECOMBINASE ACTIVITY SO SCIENCE LA English DT Article C1 USN HOSP,NCI,PEDIAT BRANCH,BETHESDA,MD 20889. NCI,DIV CANC BIOL & DIAG,PATHOL LAB,BETHESDA,MD 20892. GEORGETOWN UNIV,WASHINGTON,DC 20057. RP APLAN, PD (reprint author), USN HOSP,NCI,MED ONCOL BRANCH,BETHESDA,MD 20889, USA. RI Aplan, Peter/K-9064-2016 NR 43 TC 258 Z9 260 U1 0 U2 5 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD DEC 7 PY 1990 VL 250 IS 4986 BP 1426 EP 1429 DI 10.1126/science.2255914 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EL843 UT WOS:A1990EL84300049 PM 2255914 ER PT J AU GERFEN, CR ENGBER, TM MAHAN, LC SUSEL, Z CHASE, TN MONSMA, FJ SIBLEY, DR AF GERFEN, CR ENGBER, TM MAHAN, LC SUSEL, Z CHASE, TN MONSMA, FJ SIBLEY, DR TI D1 AND D2 DOPAMINE RECEPTOR REGULATED GENE-EXPRESSION OF STRIATONIGRAL AND STRIATOPALLIDAL NEURONS SO SCIENCE LA English DT Article C1 NINCDS,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. RP GERFEN, CR (reprint author), NIMH,CELL BIOL LAB,BLDG 36,ROOM 2D-10,BETHESDA,MD 20892, USA. NR 37 TC 2005 Z9 2028 U1 2 U2 62 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD DEC 7 PY 1990 VL 250 IS 4986 BP 1429 EP 1432 DI 10.1126/science.2147780 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EL843 UT WOS:A1990EL84300050 PM 2147780 ER PT J AU FREED, WJ AF FREED, WJ TI FETAL BRAIN GRAFTS AND PARKINSONS-DISEASE SO SCIENCE LA English DT Article RP FREED, WJ (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,WASHINGTON,DC 20032, USA. NR 10 TC 12 Z9 12 U1 0 U2 0 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD DEC 7 PY 1990 VL 250 IS 4986 BP 1434 EP 1434 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EL843 UT WOS:A1990EL84300053 ER PT J AU KUPRIYANOV, VV BALABAN, RS LYULINA, NV STEINSCHNEIDER, AY SAKS, VA AF KUPRIYANOV, VV BALABAN, RS LYULINA, NV STEINSCHNEIDER, AY SAKS, VA TI COMBINATION OF P-31-NMR MAGNETIZATION TRANSFER AND RADIOISOTOPE EXCHANGE METHODS FOR ASSESSMENT OF AN ENZYME REACTION-MECHANISM - RATE-DETERMINING STEPS OF THE CREATINE-KINASE REACTION SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Article DE CREATINE KINASE; NMR, P-31, SATURATION TRANSFER; RADIOISOTOPE EXCHANGE; SUBSTRATE-ENZYME COMPLEX; RATE DETERMINING STEP ID RESONANCE SATURATION TRANSFER; DIHYDROFOLATE-REDUCTASE; NMR; HEART; KINETICS; MUSCLE; PHOSPHATE; INVITRO; BINDING; RABBIT AB The theoretical analysis of a reversible enzyme reaction performed in this work shows that the P-31-NMR magnetization (saturation) transfer technique combined with a radioistope exchange method may potentially provide information on the position of rate-determining step(s). It depends on chemical shifts of NMR signals of nuclei of interest in free and enzyme-bound forms of substrate(s) and product(s) of the reaction. The creatine kinase reaction (MgATP + creatine half-arrow-pointing-left-and-right MgADP + P-creatine) has been used as a model. Chemical shifts of P-31 in binary, tenary and transitional state substrate-enzyme complexes have been estimated by the variable frequency saturation transfer (VFST) method. This method is based on selective irradiation of numerous points in the spectrum and observation of changes in the intensity of visible line(s) which occur due to chemical exchange between it and lines which are not visible in the routine spectrum. Also, dissociation rate constants of MgADP-containing complexes were determined. Magnetization exchange rates, P-creatine half-arrow-pointing-left-and-right [gamma-P]MgATP and [beta-P]MgADP half-arrow-pointing-left-and-right [beta-P]MgATP, were compared with radioisotope exchange rates, [gamma-P-32-MgATP half-arrow-pointing-left-and-right P-creatine and [H-3]MgADP half-arrow-pointing-left-and-right MgATP at different [P-creatine]/[creatine] ratios and at different temperatures. All these exchanges rates were close to each other at 30-37-degrees-C and [PCr]/[Cr] ratios lower than 2. It is concluded that phosphoryl group transfer is the rate-determining step of the overall creatine kinase reaction under these conditions. However, at lower temperatures (below 25-degrees-C) or at high [PCr]/[Cr] ratios ([ADP] < 20 mu-M) the rate-determining step seems to be shifted toward dissociation of nucleotide substrates from enzyme-substrate complexes, since exchange rates became significantly different. This approach is useful for analysis of mechanism of enzymatic reactions and also can be applied to non-enzymatic reactions and evaluation of small rapidly exchangeable metabolite pools. C1 NHLBI,CARDIAC ENERGET LAB,BETHESDA,MD 20892. RP KUPRIYANOV, VV (reprint author), USSR CARDIOL RES CTR,INST EXPTL CARDIOL,BIOENERGET LAB,3RD CHEREPKOVSKAYA STR 15A,MOSCOW 121552,USSR. RI SAKS, Valdur/A-8466-2008; Balaban, Robert/A-7459-2009 OI Balaban, Robert/0000-0003-4086-0948 NR 33 TC 12 Z9 12 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD DEC 6 PY 1990 VL 1020 IS 3 BP 290 EP 304 DI 10.1016/0005-2728(90)90160-6 PG 15 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EP095 UT WOS:A1990EP09500010 PM 2248962 ER PT J AU ALTSCHUL, SF LIPMAN, DJ AF ALTSCHUL, SF LIPMAN, DJ TI EQUAL ANIMALS SO NATURE LA English DT Letter RP ALTSCHUL, SF (reprint author), NIH,NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20894, USA. NR 7 TC 30 Z9 31 U1 0 U2 7 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD DEC 6 PY 1990 VL 348 IS 6301 BP 493 EP 494 DI 10.1038/348493c0 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EL760 UT WOS:A1990EL76000042 PM 2247160 ER PT J AU HORAK, ID POPOVIC, M HORAK, EM LUCAS, PJ GRESS, RE JUNE, CH BOLEN, JB AF HORAK, ID POPOVIC, M HORAK, EM LUCAS, PJ GRESS, RE JUNE, CH BOLEN, JB TI NO T-CELL TYROSINE PROTEIN-KINASE SIGNALING OR CALCIUM MOBILIZATION AFTER CD4 ASSOCIATION WITH HIV-1 OR HIV-1 GP120 SO NATURE LA English DT Article C1 NCI,TUMOR VIRUS BIOL LAB,BLDG 41 ROOM D824,BETHESDA,MD 20892. NCI,MED BRANCH,BETHESDA,MD 20892. NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. USN,MED RES INST,BETHESDA,MD 20814. NEW MEXICO STATE UNIV,DEPT VIROL,PRIMATE RES INST,HOLLOMAN AFB,NM 88330. NR 19 TC 92 Z9 92 U1 0 U2 1 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD DEC 6 PY 1990 VL 348 IS 6301 BP 557 EP 560 DI 10.1038/348557a0 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EL760 UT WOS:A1990EL76000070 PM 1701034 ER PT J AU RAUB, W AF RAUB, W TI EARLY EXPOSURE TO HOUSE DUST MITES MAY LEAD TO ASTHMA SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BETHESDA,MD 20892, USA. NR 1 TC 1 Z9 1 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 5 PY 1990 VL 264 IS 21 BP 2731 EP 2731 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EK537 UT WOS:A1990EK53700010 PM 2232052 ER PT J AU RAUB, W AF RAUB, W TI CHRONIC FETAL HYPOXIA MAY PREDISPOSE INFANTS TO SUDDEN-INFANT-DEATH-SYNDROME SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BETHESDA,MD 20892, USA. NR 1 TC 1 Z9 1 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 5 PY 1990 VL 264 IS 21 BP 2731 EP 2731 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EK537 UT WOS:A1990EK53700009 PM 2232052 ER PT J AU RAUB, W AF RAUB, W TI NEW COMPUTER TECHNIQUE MAY LEAD TO NEW AIDS DRUGS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BETHESDA,MD 20892, USA. NR 1 TC 1 Z9 1 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 5 PY 1990 VL 264 IS 21 BP 2731 EP 2731 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EK537 UT WOS:A1990EK53700007 PM 2232052 ER PT J AU RAUB, W AF RAUB, W TI NEUROENDOCRINE-CELL HYPERPLASIA MAY BE INVOLVED IN EOSINOPHILIC GRANULOMA SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP RAUB, W (reprint author), NIH,BETHESDA,MD 20892, USA. NR 1 TC 1 Z9 1 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 5 PY 1990 VL 264 IS 21 BP 2731 EP 2731 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EK537 UT WOS:A1990EK53700008 PM 2232052 ER PT J AU JAKOBY, WB ZIEGLER, DM AF JAKOBY, WB ZIEGLER, DM TI THE ENZYMES OF DETOXICATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Review C1 UNIV TEXAS,DEPT CHEM,AUSTIN,TX 78712. UNIV TEXAS,CLAYTON FDN,INST BIOCHEM,AUSTIN,TX 78712. RP JAKOBY, WB (reprint author), NIDDKD,BIOCHEM LAB,BETHESDA,MD 20892, USA. NR 57 TC 288 Z9 292 U1 0 U2 12 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 5 PY 1990 VL 265 IS 34 BP 20715 EP 20718 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EL020 UT WOS:A1990EL02000001 PM 2249981 ER PT J AU YAMAKI, K TSUDA, M KIKUCHI, T CHEN, KH HUANG, KP SHINOHARA, T AF YAMAKI, K TSUDA, M KIKUCHI, T CHEN, KH HUANG, KP SHINOHARA, T TI STRUCTURAL ORGANIZATION OF THE HUMAN S-ANTIGEN GENE - CDNA, AMINO-ACID, INTRON, EXON, PROMOTER, INVITRO TRANSCRIPTION, RETINA, AND PINEAL-GLAND SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article C1 NEI,RETINAL CELL & MOLEC BIOL LAB,MOLEC BIOL SECT,BLDG 10,RM 10N117,BETHESDA,MD 20892. NICHHD,ENDOCRINOL & REPROD RES BRANCH,BETHESDA,MD 20892. OI Shinohara, Toshimichi/0000-0002-7197-9039 NR 46 TC 58 Z9 58 U1 0 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 5 PY 1990 VL 265 IS 34 BP 20757 EP 20762 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EL020 UT WOS:A1990EL02000009 PM 2249983 ER PT J AU WILLIAMSON, KC SMITH, LA MOSS, J VAUGHAN, M AF WILLIAMSON, KC SMITH, LA MOSS, J VAUGHAN, M TI GUANINE NUCLEOTIDE-DEPENDENT ADP-RIBOSYLATION OF SOLUBLE RHO CATALYZED BY CLOSTRIDIUM-BOTULINUM C3 ADP-RIBOSYLTRANSFERASE - ISOLATION AND CHARACTERIZATION OF A NEWLY RECOGNIZED FORM OF RHOA SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article C1 USA,MED RES INST INFECT DIS,DIV PATHOL,FREDERICK,MD 21701. RP WILLIAMSON, KC (reprint author), NHLBI,CELLULAR METAB LAB,BLDG 10,RM 5N307,BETHESDA,MD 20892, USA. NR 41 TC 15 Z9 16 U1 1 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 5 PY 1990 VL 265 IS 34 BP 20807 EP 20812 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EL020 UT WOS:A1990EL02000016 PM 2174426 ER PT J AU KAMBADUR, R LEWIS, M CHANG, SL FLAVIN, M AF KAMBADUR, R LEWIS, M CHANG, SL FLAVIN, M TI CHARACTERIZATION OF PUTATIVE CYTOSKELETAL PROTEINS FROM A TRYPANOSOMATID AND THEIR COMPARATIVE BINDING TO MICROTUBULES AND SOLUBLE TUBULIN SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article C1 NHLBI,CELL BIOL LAB,BETHESDA,MD 20892. NIH,DIV RES SERV,BIOMED ENGN & INSTRUMENTAT BRANCH,BETHESDA,MD 20892. NR 13 TC 3 Z9 3 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 5 PY 1990 VL 265 IS 34 BP 20959 EP 20965 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EL020 UT WOS:A1990EL02000039 PM 1979074 ER PT J AU MOSS, J STANLEY, SJ LEVINE, RL AF MOSS, J STANLEY, SJ LEVINE, RL TI INACTIVATION OF BACTERIAL GLUTAMINE-SYNTHETASE BY ADP-RIBOSYLATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. RP MOSS, J (reprint author), NHLBI,CELLULAR METAB LAB,BLDG 10,RM 5N307,BETHESDA,MD 20892, USA. RI Levine, Rodney/D-9885-2011 NR 56 TC 14 Z9 14 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 5 PY 1990 VL 265 IS 34 BP 21056 EP 21060 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EL020 UT WOS:A1990EL02000055 PM 1979075 ER PT J AU MUKAIDA, N MAHE, Y MATSUSHIMA, K AF MUKAIDA, N MAHE, Y MATSUSHIMA, K TI COOPERATIVE INTERACTION OF NUCLEAR FACTOR-KAPPA-B-REGULATORY AND CIS-REGULATORY ENHANCER BINDING PROTEIN-LIKE FACTOR BINDING-ELEMENTS IN ACTIVATING THE INTERLEUKIN-8 GENE BY PRO-INFLAMMATORY CYTOKINES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article RP MUKAIDA, N (reprint author), NCI,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21702, USA. RI Mukaida, Naofumi/D-7623-2011 OI Mukaida, Naofumi/0000-0002-4193-1851 NR 51 TC 583 Z9 588 U1 0 U2 4 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 5 PY 1990 VL 265 IS 34 BP 21128 EP 21133 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EL020 UT WOS:A1990EL02000065 PM 2250017 ER PT J AU ROJAS, E POLLARD, HB HAIGLER, HT PARRA, C BURNS, AL AF ROJAS, E POLLARD, HB HAIGLER, HT PARRA, C BURNS, AL TI CALCIUM-ACTIVATED ENDONEXIN-II FORMS CALCIUM CHANNELS ACROSS ACIDIC PHOSPHOLIPID-BILAYER MEMBRANES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article C1 UNIV CALIF IRVINE,DEPT PHYSIOL & BIOPHYS,IRVINE,CA 92717. RP ROJAS, E (reprint author), NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD 20892, USA. RI Haigler, Harry/C-3788-2011 NR 35 TC 207 Z9 207 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 5 PY 1990 VL 265 IS 34 BP 21207 EP 21215 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EL020 UT WOS:A1990EL02000076 PM 2174439 ER PT J AU KADOWAKI, H KADOWAKI, T CAMA, A MARCUSSAMUELS, B ROVIRA, A BEVINS, CL TAYLOR, SI AF KADOWAKI, H KADOWAKI, T CAMA, A MARCUSSAMUELS, B ROVIRA, A BEVINS, CL TAYLOR, SI TI MUTAGENESIS OF LYSINE-460 IN THE HUMAN INSULIN-RECEPTOR - EFFECTS UPON RECEPTOR RECYCLING AND COOPERATIVE INTERACTIONS AMONG BINDING-SITES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article C1 NIDDKD,DIABET BRANCH,BETHESDA,MD 20892. NR 43 TC 94 Z9 97 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 5 PY 1990 VL 265 IS 34 BP 21285 EP 21296 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EL020 UT WOS:A1990EL02000087 PM 2123490 ER EF