FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Leroux, DJ Kerr, YH Al Bitar, A Bindlish, R Jackson, TJ Berthelot, B Portet, G AF Leroux, Delphine J. Kerr, Yann H. Al Bitar, Ahmad Bindlish, Rajat Jackson, Thomas J. Berthelot, Beatrice Portet, Gautier TI Comparison Between SMOS, VUA, ASCAT, and ECMWF Soil Moisture Products Over Four Watersheds in US SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Soil moisture; Soil Moisture and Ocean Salinity (SMOS); validation ID IN-SITU OBSERVATIONS; RETRIEVAL; VALIDATION; MISSION AB As part of the Soil Moisture and Ocean Salinity (SMOS) validation process, a comparison of the skills of three satellites [SMOS, Advanced Microwave Scanning Radiometer-Earth Observing System (AMSR-E) or Advanced Microwave Scanning Radiometer, and Advanced Scatterometer (ASCAT)], and one-model European Centre for Medium Range Weather Forecasting (ECMWF) soil moisture products is conducted over four watersheds located in the U. S. The four products compared in for 2010 over four soil moisture networks were used for the calibration of AMSR-E. The results indicate that SMOS retrievals are closest to the ground measurements with a low average root mean square error of 0.061 m (3) . m(-3) for the morning overpass and 0.067 m (3) . m(-3) for the afternoon overpass, which represents an improvement by a factor of 2-3 compared with the other products. The ECMWF product has good correlation coefficients (around 0.78) but has a constant bias of 0.1-0.2 m (3) . m(-3) over the four networks. The land parameter retrieval model AMSR-E product gives reasonable results in terms of correlation (around 0.73) but has a variable seasonal bias over the year. The ASCAT soil moisture index is found to be very noisy and unstable. C1 [Leroux, Delphine J.; Kerr, Yann H.; Al Bitar, Ahmad] Ctr Etud Spatiale Biosphere, F-31404 Toulouse, France. [Leroux, Delphine J.] Telespazio SpA, F-31404 Toulouse, France. [Bindlish, Rajat; Jackson, Thomas J.] USDA, Agr Res Ctr, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Berthelot, Beatrice] Magellium, F-31520 Toulouse, France. [Portet, Gautier] Telespazio SpA, F-31023 Toulouse, France. RP Leroux, DJ (reprint author), Ctr Etud Spatiale Biosphere, F-31404 Toulouse, France. EM delphine.leroux@cesbio.cnes.fr; yann.kerr@cesbio.cnes.fr; ahmad.albitar@cesbio.cnes.fr; rajat.bindlish@ars.usda.gov; tom.jackson@ars.usda.gov; beat-rice.berthelot@magellium.fr; gautier.portet@telespazio.com OI Leroux, Delphine/0000-0003-1688-021X FU Telespazio France; TOSCA FX This work was supported in part by Telespazio France and TOSCA. NR 30 TC 28 Z9 28 U1 5 U2 42 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 EI 1558-0644 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD MAR PY 2014 VL 52 IS 3 BP 1562 EP 1571 DI 10.1109/TGRS.2013.2252468 PG 10 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA 285PA UT WOS:000329404800003 ER PT J AU Wagner, W Brocca, L Naeimi, V Reichle, R Draper, C de Jeu, R Ryu, D Su, CH Western, A Calvet, JC Kerr, YH Leroux, DJ Drusch, M Jackson, TJ Hahn, S Dorigo, W Paulik, C AF Wagner, Wolfgang Brocca, Luca Naeimi, Vahid Reichle, Rolf Draper, Clara de Jeu, Richard Ryu, Dongryeol Su, Chun-Hsu Western, Andrew Calvet, Jean-Christophe Kerr, Yann H. Leroux, Delphine J. Drusch, Matthias Jackson, Thomas J. Hahn, Sebastian Dorigo, Wouter Paulik, Christoph TI Clarifications on the "Comparison Between SMOS, VUA, ASCAT, and ECMWF Soil Moisture Products Over Four Watersheds in US" SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Advanced Scatterometer (ASCAT); noise characterization; soil moisture; Soil Moisture and Ocean Salinity (SMOS); validation ID RETRIEVAL ALGORITHM; IN-SITU; VALIDATION AB In a recent paper, Leroux et al. compared three satellite soil moisture data sets (SMOS, AMSR-E, and ASCAT) and ECMWF forecast soil moisture data to in situ measurements over four watersheds located in the United States. Their conclusions stated that SMOS soil moisture retrievals represent "an improvement [in RMSE] by a factor of 2-3 compared with the other products" and that the ASCAT soil moisture data are "very noisy and unstable." In this clarification, the analysis of Leroux et al. is repeated using a newer version of the ASCAT data and additional metrics are provided. It is shown that the ASCAT retrievals are skillful, although they show some unexpected behavior during summer for two of the watersheds. It is also noted that the improvement of SMOS by a factor of 2-3 mentioned by Leroux et al. is driven by differences in bias and only applies relative to AMSR-E and the ECWMF data in the now obsolete version investigated by Leroux et al. C1 [Wagner, Wolfgang; Naeimi, Vahid; Hahn, Sebastian; Dorigo, Wouter; Paulik, Christoph] Vienna Univ Technol TU Wien, Dept Geodesy & Geoinformat, A-1040 Vienna, Austria. [Brocca, Luca] CNR, Res Inst Geohydrol Protect, I-06128 Perugia, Italy. [Reichle, Rolf; Draper, Clara] NASA, Goddard Space Flight Ctr, Global Modeling & Assimilat Off, Greenbelt, MD 20771 USA. [de Jeu, Richard] Free Univ Amsterdam, Dept Earth Sci, NL-1081 HV Amsterdam, Netherlands. [Ryu, Dongryeol; Su, Chun-Hsu; Western, Andrew] Univ Melbourne, Dept Infrastruct Engn, Melbourne, Vic 3010, Australia. [Calvet, Jean-Christophe] Meteo France, Ctr Natl Rech Meteorol, F-31057 Toulouse, France. [Kerr, Yann H.; Leroux, Delphine J.] Ctr Etud Spatiales Biosphere, F-31401 Toulouse, France. [Drusch, Matthias] European Space Agcy, European Space Res & Technol Ctr, NL-2201 AZ Noordwijk, Netherlands. [Jackson, Thomas J.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. RP Wagner, W (reprint author), Vienna Univ Technol TU Wien, Dept Geodesy & Geoinformat, A-1040 Vienna, Austria. EM wolfgang.wagner@geo.tuwien.ac.at; luca.brocca@irpi.cnr.it; rolf.reichle@nasa.gov; clara.draper@nasa.gov; r.a.m.de.jeu@vu.nl; dryu@unimelb.edu.au; csu@unimelb.edu.au; a.western@unimelb.edu.au; jean-christophe.calvet@meteo.fr; yann.kerr@cesbio.cnes.fr; delphine.leroux@cesbio.cnes.fr; matthias.drusch@esa.int; tom.jackson@ars.usda.gov RI Brocca, Luca/F-2854-2010; Western, Andrew/G-1730-2011; Reichle, Rolf/E-1419-2012; Su, Chun-Hsu/C-2076-2014; Ryu, Dongryeol/C-5903-2008; Draper, Clara/P-6097-2016 OI Wagner, Wolfgang/0000-0001-7704-6857; Paulik, Christoph/0000-0002-4231-7933; Dorigo, Wouter/0000-0001-8054-7572; Brocca, Luca/0000-0002-9080-260X; Western, Andrew/0000-0003-4982-146X; Su, Chun-Hsu/0000-0003-2504-0466; Ryu, Dongryeol/0000-0002-5335-6209; Draper, Clara/0000-0002-8299-4939 FU EUMETSAT through the H-SAF project; European Space Agency through the International Soil Moisture Network project FX This work was supported in part by EUMETSAT through the H-SAF project (hsaf.meteoam.it) and in part by the European Space Agency through the International Soil Moisture Network project. NR 20 TC 13 Z9 13 U1 2 U2 21 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 EI 1558-0644 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD MAR PY 2014 VL 52 IS 3 BP 1901 EP 1906 DI 10.1109/TGRS.2013.2282172 PG 6 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA 285PA UT WOS:000329404800032 ER PT J AU Knothe, G AF Knothe, Gerhard TI A comprehensive evaluation of the cetane numbers of fatty acid methyl esters SO FUEL LA English DT Article DE Biodiesel; Cetane number; Methyl esters ID ARTIFICIAL NEURAL-NETWORKS; FUEL PROPERTIES; KINEMATIC VISCOSITY; PHYSICAL-PROPERTIES; BIODIESEL FUEL; PREDICTION; OIL; EMISSIONS; PERFORMANCE; OXIDATION AB The cetane number (CN), being related to ignition and combustion behavior, is a prime indicator of the quality of diesel fuels, including those derived from renewable resources such as biodiesel. While many effects of compound structure are known or have been postulated, experimental data have not always been available for the various ester components of biodiesel and a comprehensive evaluation including some structural features not yet experimentally investigated such as double bond configuration and position is lacking. In this work, existing CN data of fatty esters are complemented by studying C18 esters with differing double bond positions and double bond configurations. For the first time, CNs, determined as derived cetane number (DCNs), of neat trans fatty acid methyl esters, methyl elaidate (methyl 9(E)octadecenoate) and methyl linolelaidate (methyl 9(E), 12(E)-octadecenoate), were determined as were the CNs of the C18: 1 positional isomers methyl petroselinate (methyl 6(Z)-octadecenoate) and methyl asclepate (methyl 11(Z)-octadecenoate). The CNs of the positional and geometric isomers of methyl oleate are close to the CN of methyl oleate. These data are compared to other previously determined CN data. Furthermore, the applicability of CN data is evaluated using an extensive collection of CN determinations for methyl oleate as "standard'' fatty acid (methyl) ester. The average CN value for methyl oleate is in the range of 56-58 with a CN of 57 suggested for calculating purposes. Similarly, uncertainty ranges could be established for CNs of other fatty materials. The data are also useful for predicting CNs of compounds for which CNs are not readily available or easily determinable. Published by Elsevier Ltd. C1 [Knothe, Gerhard] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Knothe, G (reprint author), USDA ARS NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM gerhard.knothe@ars.usda.gov NR 61 TC 31 Z9 31 U1 4 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0016-2361 EI 1873-7153 J9 FUEL JI Fuel PD MAR PY 2014 VL 119 BP 6 EP 13 DI 10.1016/j.fuel.2013.11.020 PG 8 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA 282YV UT WOS:000329212100002 ER PT J AU Hwang, CA Sheen, S Juneja, V Hwang, CF Yin, TC Chang, NY AF Hwang, Cheng-An Sheen, Shiowshuh Juneja, Vijay Hwang, Chin-Fa Yin, Tzu-Chiao Chang, Nai-Yu TI The influence of acid stress on the growth of Listeria monocytogenes and Escherichia coli O157:H7 on cooked ham SO FOOD CONTROL LA English DT Article DE Listeria monocytogenes; Escherichia coli O157:H7; Acid stress; Meat ID BEEF CARCASS SURFACES; LACTIC-ACID; SALMONELLA-TYPHIMURIUM; DECONTAMINATION TREATMENTS; HOT-WATER; STEAM PASTEURIZATION; CROSS-PROTECTION; ORGANIC-ACIDS; E.-COLI; O157-H7 AB Acid solutions are increasingly being used for decontaminating meat surfaces. On the surfaces of acid-treated meat, the population of microorganism is reduced due to the low pH of acids, and the subsequent growth of the microorganism is reduced due to the residual acids on meat surfaces. Microbial cells on meat surfaces subjected to acid treatments may cross-contaminate untreated meat surfaces, e.g., microorganisms on the surfaces of acid-treated cooked ham cross contaminate the untreated surfaces during slicing. The objective of this study was to examine this scenario in determining the subsequent growth of acid-treated Listeria monocytogenes and Escherichia coli O157:H7 on the surfaces of untreated meat. Cells of multiple-strain L monocytogenes or E. coli O157:H7 were exposed to HCI solutions of pH 3, 4, or 5 and deionized water at room temperature for 24 h. The acid or deionized water-treated cells were inoculated separately onto cooked ham. Samples inoculated with L. monocytogenes were stored at 4 and 8 degrees C and samples inoculated with E. coli O157:H7 were stored at 10 and 12 degrees C. Populations of the pathogens on ham were enumerated during storage, and the lag phase durations (LPD, h) and growth rates (GR, log CFU/h) of the pathogens were determined. The populations of L monocytogenes and E. coli O157:H7 in pH 5, 4, and 3 solutions were 1.2-3.1 and 0.6-2.4 log CFU/ml, respectively, lower than those in deionized water, indicating an increased acid stress on both microorganisms at lower pHs. L monocytogenes subjected to pH 3 and pH 4 stresses and E. coli O157:H7 subjected to pH 3 stress exhibited significantly (p < 0.05) extended LPDs and reduced GRs on cooked ham. The growth of L monocytogenes on ham was more readily reduced by acid stress than that of E. coli O157:H7. This study showed that acid treatments reduced the viability of L monocytogenes and E. coli O157:H7 and the acid stress reduced their subsequent growth ability on untreated ham. Therefore, cross-contamination of L monocytogenes or E. coli O157:H7 cells from acid-treated meat surfaces onto untreated meat surfaces may not impose increased risk to the product. Published by Elsevier Ltd. C1 [Hwang, Cheng-An; Juneja, Vijay] ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Sheen, Shiowshuh] ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Hwang, Chin-Fa; Yin, Tzu-Chiao; Chang, Nai-Yu] Hungkuang Univ, Dept Food Sci & Technol, Taichung 43302, Taiwan. RP Hwang, CA (reprint author), ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM andy.hwang@ars.usda.gov NR 47 TC 2 Z9 2 U1 3 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD MAR PY 2014 VL 37 BP 245 EP 250 DI 10.1016/j.foodcont.2013.09.040 PG 6 WC Food Science & Technology SC Food Science & Technology GA 273EQ UT WOS:000328518200039 ER PT J AU Mendoza, F Lu, RF Cen, HY AF Mendoza, Fernando Lu, Renfu Cen, Haiyan TI Grading of apples based on firmness and soluble solids content using Vis/SWNIR spectroscopy and spectral scattering techniques SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Apples; Firmness; Soluble solids content; Sorting; Near-infrared spectroscopy; Spectral scattering ID FRUIT FIRMNESS; PREDICTING FIRMNESS; QUALITY; PREFERENCES AB Sorting of apple fruit based on internal quality can enhance the industry's competiveness and profitability and assure consumer satisfaction. In this research, visible and shortwave near-infrared (Vis/SWNIR) spectroscopy (460-1100 nm) and spectral scattering (450-1050 nm) were used for sorting three varieties of apple (i.e., 'Delicious', 'Golden Delicious' and 'Jonagold') into two quality grades based on firmness, soluble solids content (SSC), or both firmness and SSC. Vis/SWNIR spectra were obtained in interactance mode under stationery condition, whereas spectral scattering images were acquired online at a conveyor speed of 82 mm/s. A total of 8491 apples for the three varieties harvested from the same orchard in 2009, 2010 and 2011 were used for analysis. First derivative spectra were obtained from the Vis/SWNIR data, while the scattering images were first preprocessed by computing mean reflectance spectra and then performing continuous wavelet transform decompositions. Sorting algorithms were developed using sequential forward selection and linear discriminant analysis, and the classification models were compared in terms of their overall performance and local confusion matrices. A sensitivity analysis was also performed to assess the effect of using different quality threshold criteria on the sorting performance. Overall, consistent and relatively good sorting results for firmness (ranging between 77.9% and 98.2%) and moderate results for SSC (ranging between 62.0% and 91.7%) were obtained using scattering technique. Vis/SWNIR technique showed slightly better sorting results for firmness (ranging between 87.3% and 97.6%) and SSC (ranging between 77.1% and 92.3%). When the classification was performed based on both firmness and SSC, the sorting accuracies generally decreased to between 75.7% and 90.1% for Vis/SWNIR and between 69.7% and 91.5% for spectral scattering. Vis/SWNIR and spectral scattering techniques have potential for online sorting and grading of apples by firmness and SSC. Published by Elsevier Ltd. C1 [Mendoza, Fernando; Lu, Renfu] Michigan State Univ, USDA ARS, E Lansing, MI 48824 USA. [Cen, Haiyan] Michigan State Univ, Dept Biosyst & Agr Engn, E Lansing, MI 48824 USA. RP Mendoza, F (reprint author), Michigan State Univ, USDA ARS, 524 S Shaw Lane,Room 105A-224, E Lansing, MI 48824 USA. EM fernando.mendoza@ars.usda.edu NR 22 TC 15 Z9 18 U1 5 U2 34 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD MAR PY 2014 VL 125 BP 59 EP 68 DI 10.1016/j.jfoodeng.2013.10.022 PG 10 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 275EY UT WOS:000328660300008 ER PT J AU Lundberg, B Pan, XJ White, A Chau, H Hotchkiss, A AF Lundberg, Brock Pan, Xuejun White, Andre Chau, Hoa Hotchkiss, Arland TI Rheology and composition of citrus fiber SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Citrus fibers; Composition; Rheology; Water-holding capacity; Viscosity ID DIETARY FIBER; PARTICLE-SIZE; HYDRATION PROPERTIES; FRUIT JUICES; FOODS; TEMPERATURE; VEGETABLES; CAPACITY; TEXTURE; BINDING AB While fibrous byproducts are abundant, using them in food products to improve food nutrition and quality without degrading taste or texture can be challenging. Citrus fiber has been shown to have high water holding capacity and apparent viscosity. However, to better incorporate citrus fiber into foods, their rheological properties, and composition, need to be better understood. Pectin was found to be 42% of the composition of the citrus fiber evaluated in this study. The theological properties of citrus fiber solutions were clearly non Newtonian and the type of model that best fit the citrus fiber varied depending on its particle size. Particle size of citrus fiber also significantly changed the apparent viscosity of their solutions. As citrus fibers hydrate, the fibers swell, which was illustrated by microscopic imaging. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Lundberg, Brock; Pan, Xuejun] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Lundberg, Brock] Fiberstar Inc, River Falls, WI 54022 USA. [White, Andre; Chau, Hoa; Hotchkiss, Arland] USDA ARS, Eastern Reg Res Ctr, Dairy & Funct Foods Res Unit, Wyndmoor, PA 19038 USA. RP Lundberg, B (reprint author), Univ Wisconsin, Dept Biol Syst Engn, 460 Henry Mall, Madison, WI 53706 USA. EM brocklundberg38@gmail.com NR 37 TC 4 Z9 5 U1 6 U2 62 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD MAR PY 2014 VL 125 BP 97 EP 104 DI 10.1016/j.jfoodeng.2013.10.021 PG 8 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 275EY UT WOS:000328660300012 ER PT J AU Sun, JH Liu, XJ Yang, TB Slovin, J Chen, P AF Sun, Jianghao Liu, Xianjin Yang, Tianbao Slovin, Janet Chen, Pei TI Profiling polyphenols of two diploid strawberry (Fragaria vesca) inbred lines using UHPLC-HRMSn SO FOOD CHEMISTRY LA English DT Article DE Strawberry; Fragaria vesca; UHPLC; HRMS; Flavonoids; Anthocyanins ID X ANANASSA DUCH.; PHENOLIC-COMPOUNDS; ANTIOXIDANT CAPACITY; MASS-SPECTROMETRY; FRUIT; QUALITY; ELECTROPHORESIS; METABOLITES; CULTIVARS; STORAGE AB Phenolic compounds in the fruits of two diploid strawberries (Fragaria vesca f. semperflorens) inbred lines-Ruegen F7-4 (a red-fruited genotype) and YW5AF7 (a yellow-fruited genotype) were characterised using ultra-high-performance liquid chromatography coupled with tandem high-resolution mass spectrometry (UHPLC-HRMSn). The changes of anthocyanin composition during fruit development and between Ruegen F7-4 and YW5AF7 were studied. About 67 phenolic compounds, including taxifolin 3-O-arabinoside, glycosides of quercetin, kaempferol, cyanidin, pelargonidin, peonidin, ellagic acid derivatives, and other flavonols were identified in these two inbred lines. Compared to the regular octoploid strawberry, unique phenolic compounds were found in F. vesca fruits, such as taxifolin 3-O-arabinoside (both) and peonidin 3-O-malonylglucoside (Ruegen F7-4). The results provide the basis for comparative analysis of polyphenolic compounds in yellow and red diploid strawberries, as well as with the cultivated octoploid strawberries. Published by Elsevier Ltd. C1 [Sun, Jianghao; Chen, Pei] ARS, USDA, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA. [Liu, Xianjin] Jiangsu Acad Agr Sci, Inst Food Safety & Qual, Nanjing, Jiangsu, Peoples R China. [Yang, Tianbao] ARS, USDA, Food Qual Lab, Beltsville, MD 20705 USA. [Slovin, Janet] ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. RP Chen, P (reprint author), ARS, USDA, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA. EM pei.chen@ars.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture; Office of Dietary Supplements (ODS) of the National Institutes of Health (NIH) FX This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements (ODS) of the National Institutes of Health (NIH). NR 28 TC 24 Z9 25 U1 5 U2 57 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD MAR 1 PY 2014 VL 146 BP 289 EP 298 DI 10.1016/j.foodchem.2013.08.089 PG 10 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 267LV UT WOS:000328100200041 PM 24176345 ER PT J AU Fu, XP Kim, MS Chao, KL Qin, JW Lim, J Lee, H Garrido-Varo, A Perez-Marin, D Ying, YB AF Fu, Xiaping Kim, Moon S. Chao, Kuanglin Qin, Jianwei Lim, Jongguk Lee, Hoyoung Garrido-Varo, Ana Perez-Marin, Dolores Ying, Yibin TI Detection of melamine in milk powders based on NIR hyperspectral imaging and spectral similarity analyses SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Food safety; Hyperspectral imaging; Milk powder adulteration; Melamine; Spectral similarity analysis ID DAIRY-PRODUCTS; INFANT FORMULA; FOOD SAFETY; SPECTROSCOPY; SYSTEM; SPECTROMETRY; REFLECTANCE; QUALITY AB Melamine (2,4,6-triamino-1,3,5-triazine) contamination of food has become an urgent and broadly recognized topic as a result of several food safety scares in the past five years. Hyperspectral imaging techniques that combine the advantages of spectroscopy and imaging have been widely applied for a variety of food quality and safety evaluations. In this study, near-infrared (NIR) hyperspectral imaging technique was investigated to detect low levels (<= 1.0%) of melamine particles in milk powders. Following image preprocessing (normalization and background removal), the spectrum of each pixel in the sample images was compared to the pure melamine spectrum by spectral similarity measures including spectral angle measure (SAM), spectral correlation measure (SCM), and Euclidian distance measure (EDM). The three similarity analysis methods provided comparable results for melamine particle detection where imaging allowed visualization of the distribution of melamine particles within images of milk powder mixture samples that were prepared with various melamine concentrations. The classification results were verified by spectral feature comparison between separated mean spectra of melamine pixels and milk powder pixels. The study demonstrated that a combination of NIR hyperspectral imaging technique and spectral similarity analyses was an effective method for melamine adulteration discrimination in milk powders. The method described in this study can also be applied to other chemicals or multi-chemicals adulterant detection in milk powders. Published by Elsevier Ltd. C1 [Fu, Xiaping; Ying, Yibin] Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Zhejiang, Peoples R China. [Fu, Xiaping; Kim, Moon S.; Chao, Kuanglin; Qin, Jianwei; Lee, Hoyoung] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Lim, Jongguk] Rural Dev Adm, Natl Inst Agr Engn, Suwon 441100, South Korea. [Garrido-Varo, Ana; Perez-Marin, Dolores] Univ Cordoba, Fac Agr & Forestry Engn, E-14080 Cordoba, Spain. RP Kim, MS (reprint author), ARS, Environm Microbial & Food Safety Lab, BARC East, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM moon.kim@ars.usda.gov FU Agenda Program, Rural Development Administration, Suwon, Korea [PJ009399] FX Authors are grateful to Ms. Diane Chan of the Environmental Microbial and Food Safety Laboratory, ARS, USDA for technical review of the manuscript and insightful suggestions. This work was partially supported by a grant from the Agenda Program (PJ009399), Rural Development Administration, Suwon, Korea. NR 29 TC 39 Z9 41 U1 13 U2 124 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD MAR PY 2014 VL 124 BP 97 EP 104 DI 10.1016/j.jfoodeng.2013.09.023 PG 8 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 268OV UT WOS:000328181300012 ER PT J AU Yi, J Li, Y Zhong, F Yokoyama, W AF Yi, Jiang Li, Yue Zhong, Fang Yokoyama, Wallace TI The physicochemical stability and in vitro bioaccessibility of beta-carotene in oil-in-water sodium caseinate emulsions SO FOOD HYDROCOLLOIDS LA English DT Article DE Beta-carotene; Sodium caseinate; Physicochemical stability; Lipolysis; Bioaccessibility ID PANCREATIC LIPASE; LIPID DROPLETS; PARTICLE-SIZE; NANOEMULSIONS; DIGESTIBILITY; PROTEIN; HOMOGENIZATION; TRIGLYCERIDES; EMULSIFIERS; DIGESTION AB Beta-carotene (BC), the most important dietary source of provitamin A, is necessary for optimum human health. BC is insoluble or only slightly soluble in most liquids but its bioavailability improves when ingested with fat. Therefore lipid emulsions are ideal matrices for BC delivery. BC (0.1%) in corn oil, the dispersed phase (5 or 10%), was homogenized with 2% sodium caseinate solution in a microfluidizer. Homogenization at different pressures produced droplet diameters (Dz = 368-124 nm) that were linear and inversely related to homogenization pressures in the pressure range 10-100 MPa. Nanoemulsions (r < 200 nm) were prepared at 100 MPa. The sodium caseinate emulsions were generally very stable to coalescence or flocculation over 30 days and the slow rate of volume increase was found to be related to the square of the initial droplet radius following Stokes velocity of settling equation. BC stability towards oxidation was lower as droplet diameter decreased. The extent of lipolysis in an in vitro system was higher and linearly related to the inverse of droplet diameter. Bioaccessibility, as defined by the amount of BC recovered in the aqueous phase after ultracentrifugation, was linearly related to smaller emulsion droplet diameter. These results show that sodium caseinate, a food grade emulsifier, can be used to prepare stable emulsions of food oils carrying beta-carotene. Since food oils facilitate the uptake of beta-carotene this may be a preferred system to deliver beta-carotene. Published by Elsevier Ltd. C1 [Yi, Jiang; Li, Yue; Zhong, Fang] Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China. [Yi, Jiang; Yokoyama, Wallace] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Zhong, F (reprint author), Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China. EM fzhong@jiangnan.edu.cn; Wally.Yokoyama@ARS.USDA.GOV FU National 125 Program [2011BAD23B02, 2013AA1022207]; NSFC [31171686]; 111 Project [B07029, PCSIRT0627]; [NSF-Jiangsu-BK2012559] FX This work was financially supported by National 125 Program 2011BAD23B02, 2013AA1022207; NSFC 31171686; NSF-Jiangsu-BK2012559; 111 Project B07029 and PCSIRT0627. NR 39 TC 48 Z9 51 U1 17 U2 122 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD MAR PY 2014 VL 35 BP 19 EP 27 DI 10.1016/j.foodhyd.2013.07.025 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 242VA UT WOS:000326270000003 ER PT J AU Cirre, J Al-Assaf, S Phillips, GO Yadav, MP Hicks, KB AF Cirre, Jesus Al-Assaf, Saphwan Phillips, Glyn O. Yadav, Madhav P. Hicks, Kevin B. TI Improved emulsification performance of corn fibre gum following maturation treatment SO FOOD HYDROCOLLOIDS LA English DT Article DE CFG; Molecular weight; High pressure homogenization; Emulsion; Adsorbed fraction ID ACACIA SEN SUPER-GUM(TM); WILLD. VAR. SENEGAL; SUGAR-BEET PECTIN; ARABINOGALACTAN; PROTEIN; SEYAL AB Corn fibre gum (CFG) in the solid state (milled powder form) was subjected to a maturation treatment by heating under atmospheric pressure at 110 degrees C for 5 (CFG5) and 24 h (CFG24). The treatment reduced the solubility and aggregation of the proteinaceous component increased with heating time. The protein content of CFG is comparable to that of gum arabic but has a different amino acids profile. Glutamine and proline are the most abundant in CFG compared with hydroxyproline and serine in gum arabic. The control and matured CFG samples have been characterized by gel permeation chromatography coupled on line to a multi angle laser light, refractive index and UV detectors. The weight average molecular weight (M-w) values for control, CFG5 and CFG24 were 4.2, 5.8 and 5.7 x 10(5) g/mol respectively with corresponding Rg values of 29, 44 and 41 nm. High pressure homogenization treatment of the control gum did not show significant changes whereas the matured samples were disaggregated. The emulsification performance and stability of the matured samples were greatly improved in comparison with the control gum. A three times increase in the proportion of the adsorbed fraction on to the oilewater interface accounts for the improved emulsification. A model to describe the changes following maturation and the improved emulsion stability is proposed. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Cirre, Jesus; Al-Assaf, Saphwan; Phillips, Glyn O.] Glyndwr Univ, Glyn O Phillips Hydrocolloids Res Ctr, Wrexham LL11 2AW, Wales. [Yadav, Madhav P.; Hicks, Kevin B.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Phillips, Glyn O.] Phillips Hydrocolloid Res Ltd, London W1S 4AQ, England. RP Al-Assaf, S (reprint author), Glyndwr Univ, Glyn O Phillips Hydrocolloids Res Ctr, Wrexham LL11 2AW, Wales. EM s.alassaf@glyndwr.ac.uk NR 16 TC 4 Z9 4 U1 1 U2 49 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD MAR PY 2014 VL 35 BP 122 EP 128 DI 10.1016/j.foodhyd.2013.05.002 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 242VA UT WOS:000326270000015 ER PT J AU Espitia, PJP Du, WX Avena-Bustillos, RD Soares, NDF McHugh, TH AF Perez Espitia, Paula Judith Du, Wen-Xian Avena-Bustillos, Roberto de Jesus Ferreira Soares, Nilda de Fatima McHugh, Tara H. TI Edible films from pectin: Physical-mechanical and antimicrobial properties - A review SO FOOD HYDROCOLLOIDS LA English DT Review DE Pectin; Biopolymer; Active packaging; Mechanical resistance; Structural characterization; Antimicrobial activity ID WATER-VAPOR PERMEABILITY; FOOD-PACKAGING APPLICATIONS; ESCHERICHIA-COLI O157-H7; BETA-LACTOGLOBULIN FILMS; ESSENTIAL OILS; LISTERIA-MONOCYTOGENES; APPLE FILMS; ANTIBACTERIAL PROPERTIES; BARRIER PROPERTIES; STORAGE STABILITY AB Pectin is one of the main components of the plant cell wall chemically constituted by poly alpha 1-4-galacturonic acids. According to its degree of esterification with methanol, pectin can be classified as high methoxyl pectin or low methoxyl pectin. In food industry, pectin is listed as generally recognized as safe (GRAS) by the Food and Drug Administration and is used as gelling, stabilizing, or thickening agent in food products such as jams, yoghurt drinks, fruity milk drinks, and ice cream. Due to its biodegradability, biocompatibility, edibility, and versatile chemical and physical properties (such as gelation, selective gas permeability, etc), pectin is a suitable polymeric matrix for the elaboration of edible films intended as active food packaging. Active packaging is a packaging system which possesses attributes beyond basic barrier properties that are achieved by adding active ingredients in the packaging material and/or using functionally active polymers. When the packaging system has antimicrobial activity, the packaging limits or prevents the microbial growth by extending the lag period and reducing the growth rate of microorganisms. This review describes the main methods for elaborating pectin edible films, principal characterization techniques for determining their physical-mechanical properties, and applications of pectin edible films as antimicrobial food packaging. Finally, legislation and future trends regarding the use of pectin edible films are also discussed. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Perez Espitia, Paula Judith; Ferreira Soares, Nilda de Fatima] Univ Fed Vicosa, Dept Food Technol, Food Packaging Lab, BR-36570000 Vicosa, MG, Brazil. [Du, Wen-Xian; Avena-Bustillos, Roberto de Jesus; McHugh, Tara H.] USDA ARS, Western Reg Res Ctr, Proc Foods Res Unit, Albany, CA 94710 USA. RP Espitia, PJP (reprint author), Univ Fed Vicosa, Dept Food Technol, Food Packaging Lab, Av PH Rolfs S-N,Campus Univ, BR-36570000 Vicosa, MG, Brazil. EM perez.espitia@gmail.com RI espitia, paula/L-6606-2013 OI espitia, paula/0000-0001-6262-0852 FU Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) FX Doctoral scholarship from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES). NR 98 TC 52 Z9 56 U1 17 U2 318 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD MAR PY 2014 VL 35 BP 287 EP 296 DI 10.1016/j.foodhyd.2013.06.005 PG 10 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 242VA UT WOS:000326270000034 ER PT J AU Galant, AL Luzio, GA Widmer, WW Cameron, RG AF Galant, Ashley L. Luzio, Gary A. Widmer, Wilbur W. Cameron, Randall G. TI Compositional and structural characterization of pectic material from Frozen Concentrated Orange Juice SO FOOD HYDROCOLLOIDS LA English DT Article DE Pectin; Galacturonic acid; Orange juice; Juice cloud; Arabinogalactan; Citrus sinensis ID RHAMNOGALACTURONAN-I; ARABINOGALACTAN-PROTEIN; MODEL HOMOGALACTURONAN; FRUIT JUICES; ENZYME MODE; CELL-WALLS; CITRUS; CLOUD; PH; METHYLESTERASE AB In citrus juices pectin is one of the major components of the suspended cloud material that imparts desirable appearance, texture and flavor. Citrus pectin is a structurally diverse polysaccharide with a backbone of alpha-(1,4)-galacturonic acid (GalA) residues, a variable proportion of which may be methylesterified, and interspersed with regions containing repeating rhamnogalacturonan dimers. We have acid extracted pectin from commercial Frozen Concentrated Orange Juice (FCOJ) and assessed its macromolecular properties, including soluble sugar composition and molecular weight as well as its degree (DM) and pattern (DB, DBabs) of methyl-esterification. The extracted material was composed mainly of polysaccharides (82%) dominated by galacturonic acid (39.2%), arabinose (13.2%) and galactose (25.6%). Protein accounted for approximately 4% of the extracted material and included arabinogalactan protein (AGP). The Number Average Molecular Weight (M-n) of the extracted material as determined by MALLS-SEC was estimated at 1.462 x 10(6) Da indicating the presence of aggregates. The pectin DM was relatively high at 74.7% with very few contiguous demethylesterified GalA residues (DB = 15.92, DBabs = 4.03). Treatment with various pectinolytic, hemicellulytic, or proteolytic enzymes allowed us to evaluate the contribution of various structural domains to the macrostructural architecture of the extracted material. Various enzyme treatments of the extract produced of a second, lower M-n peak and a loss of material in the larger M-n peak. The M-n varied according to the enzymatic digestion method employed, from 1.5 x 10(6) Da for the parent material, to 1.4 x 10(3) Da when treated with Rapidase Adex-P. Published by Elsevier Ltd. C1 [Galant, Ashley L.; Luzio, Gary A.; Widmer, Wilbur W.; Cameron, Randall G.] USDA ARS, Citrus & Other Subtrop Prod Res Unit, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Cameron, RG (reprint author), USDA ARS, Citrus & Other Subtrop Prod Res Unit, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM Randall.Cameron@ars.usda.gov FU ARS CRIS [6621-41000-016=00D]; USDA FX This research was supported by USDA, ARS CRIS 6621-41000-016=00D and the USDA, ARS Postdoctoral Research Associate Program - Class of 2011. These Funding Sources had no role in study design; in the collection, analysis, and interpretation of data; in the writing of the report; and in the decision to submit the paper for publication. NR 69 TC 3 Z9 3 U1 3 U2 48 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD MAR PY 2014 VL 35 BP 661 EP 669 DI 10.1016/j.foodhyd.2013.08.012 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 242VA UT WOS:000326270000077 ER PT J AU Dubey, JP Hotea, I Olariu, TR Jones, JL Darabus, G AF Dubey, J. P. Hotea, I. Olariu, T. R. Jones, J. L. Darabus, G. TI Epidemiological review of toxoplasmosis in humans and animals in Romania SO PARASITOLOGY LA English DT Article DE congenital; humans; clinical; Toxoplasma gondii; Romania; toxoplasmosis; animals ID RISK-FACTORS; GONDII; INFECTION; SEROPREVALENCE; PREVALENCE; CATS AB Infections by the protozoan parasite Toxoplasma gondii are widely prevalent in humans and other animals worldwide. However, information from eastern European countries is sketchy. In many eastern European countries, including Romania, it has been assumed that chronic T. gondii infection is a common cause of infertility and abortion. For this reason, many women in Romania with these problems were needlessly tested for T. gondii infection. Most papers on toxoplasmosis in Romania were published in Romanian in local journals and often not available to scientists in other countries. Currently, the rate of congenital infection in Romania is largely unknown. In addition, there is little information on genetic characteristics of T. gondii or prevalence in animals and humans in Romania. In the present paper we review prevalence, clinical spectrum and epidemiology of T. gondii in humans and animals in Romania. This knowledge should be useful to biologists, public health workers, veterinarians and physicians. C1 [Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab,BARC East, Beltsville, MD 20705 USA. [Hotea, I.; Darabus, G.] Banats Univ Agr Sci & Vet Med, Fac Vet Med, Dept Parasitol, Timisoara 300645, Timis, Romania. [Olariu, T. R.] Victor Babes Univ Med & Pharm, Dept Parasitol, Timisoara 300041, Timis, Romania. [Jones, J. L.] Ctr Dis Control & Prevent, Parasit Dis Branch, Div Parasit Dis & Malaria, Ctr Global Hlth, Atlanta, GA 30333 USA. RP Dubey, JP (reprint author), ARS, USDA, APDL, BARC East, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov; hotea_ionela@yahoo.com RI Hotea, Ionela Luminta/C-5100-2012 OI Hotea, Ionela Luminta/0000-0003-4812-470X NR 117 TC 7 Z9 9 U1 3 U2 13 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 EI 1469-8161 J9 PARASITOLOGY JI Parasitology PD MAR PY 2014 VL 141 IS 3 BP 311 EP 325 DI 10.1017/S0031182013001509 PG 15 WC Parasitology SC Parasitology GA AC2WV UT WOS:000332377900001 PM 24553077 ER PT J AU Almeida, GFD Thamsborg, SM Madeira, AMBN Ferreira, JFS Magalhaes, PM Dematte, LC Horsted, K Hermansen, JE AF Almeida, Gustavo F. D. Thamsborg, Stig M. Madeira, Alda M. B. N. Ferreira, Jorge F. S. Magalhaes, Pedro M. Dematte Filho, Luiz C. Horsted, Klaus Hermansen, John E. TI The effects of combining Artemisia annua and Curcuma longa ethanolic extracts in broilers challenged with infective oocysts of Eimeria acervulina and E. maxima SO PARASITOLOGY LA English DT Article DE plant extract; broiler; drug combination; coccidiostats; natural anti-protozoa drugs; herbal medicine ID ANTICOCCIDIAL DRUGS; AVIAN COCCIDIOSIS; MALARIA THERAPY; PLANT-EXTRACT; CHICKENS; COMBINATION; EFFICACY; TENELLA; ANTIMALARIAL; PERFORMANCE AB Due to an increasing demand for natural products to control coccidiosis in broilers, we investigated the effects of supplementing a combination of ethanolic extracts of Artemisia annua and Curcuma longa in drinking water. Three different dosages of this herbal mixture were compared with a negative control (uninfected), a positive control (infected and untreated), chemical coccidiostats (nicarbazin+narazin and, later, salinomycin), vaccination, and a product based on oregano. Differences in performance (weight gain, feed intake, and feed conversion rate), mortality, gross intestinal lesions and oocyst excretion were investigated. Broilers given chemical coccidiostats performed better than all other groups. Broilers given the two highest dosages of the herbal mixture had intermediate lesion scores caused by Eimeria acervulina, which was higher than in broilers given coccidiostats, but less than in broilers given vaccination, oregano and in negative controls. There was a trend for lower mortality (P = 0 center dot 08) in the later stage of the growing period (23-43 days) in broilers given the highest dosage of herbal mixture compared with broilers given chemical coccidiostats. In conclusion, the delivery strategy of the herbal extracts is easy to implement at farm level, but further studies on dose levels and modes of action are needed. C1 [Almeida, Gustavo F. D.; Horsted, Klaus; Hermansen, John E.] Aarhus Univ, Dept Agroecol, Fac Sci & Technol, Res Ctr Foulum, DK-8830 Tjele, Denmark. [Thamsborg, Stig M.] Univ Copenhagen, Danish Ctr Expt Parasitol, Fac Life Sci, DK-1870 Frederiksberg C, Denmark. [Madeira, Alda M. B. N.] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo, Brazil. [Ferreira, Jorge F. S.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Magalhaes, Pedro M.] Univ Estadual Campinas, UNICAMP, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, BR-13081970 Campinas, SP, Brazil. [Dematte Filho, Luiz C.] Univ Sao Paulo, Dept Econ & Rural Sociol, Luiz de Queiroz Coll Agr ESALQ, Piracicaba, SP, Brazil. RP Almeida, GFD (reprint author), Aarhus Univ, Dept Agroecol, Fac Sci & Technol, Res Ctr Foulum, Blichers Alle 20,POB 50, DK-8830 Tjele, Denmark. EM Gustavo.deAlmeida@agrsci.dk RI Hermansen, John /F-2247-2016; OI Hermansen, John /0000-0003-0179-2647; Ferreira, Jorge F.S./0000-0003-4550-6761 FU Danish Ministry of Science, Technology and Innovation; Aarhus University; SOAR - Research School for Organic Agriculture and Food Systems; Korin Agropecuaria Ltd. FX The Danish Ministry of Science, Technology and Innovation is thanked for financing collaboration between Brazilian and Danish scientists. Aarhus University, SOAR - Research School for Organic Agriculture and Food Systems and Korin Agropecuaria Ltd. - are thanked for supporting our study. NR 46 TC 3 Z9 3 U1 0 U2 10 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 EI 1469-8161 J9 PARASITOLOGY JI Parasitology PD MAR PY 2014 VL 141 IS 3 BP 347 EP 355 DI 10.1017/S0031182013001443 PG 9 WC Parasitology SC Parasitology GA AC2WV UT WOS:000332377900004 PM 24553078 ER PT J AU Vadiveloo, M Scott, M Quatromoni, P Jacques, P Parekh, N AF Vadiveloo, Maya Scott, Marc Quatromoni, Paula Jacques, Paul Parekh, Niyati TI Trends in dietary fat and high-fat food intakes from 1991 to 2008 in the Framingham Heart Study participants SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Dietary fats; Trends; Framingham Heart Study; Longitudinal studies ID FREQUENCY QUESTIONNAIRE; US ADULTS; NUTRITION; CONSUMPTION; PREVALENCE; NUTRIENTS; OBESITY; DESIGN; COHORT; ACIDS AB Few longitudinal studies carried out in US adults have evaluated long-term dietary fat intakes and compared them with the national recommendations during the two-decade period when the prevalence of obesity and insulin resistance increased substantively. In the present study, we examined trends in the intakes of dietary fats and rich dietary sources of fats in the Framingham Heart Study Offspring Cohort over a 17-year period. The cohort was established in 1971-75 with follow-up examinations being conducted approximately every 4 years. Dietary data were collected using a semi-quantitative FFQ beginning in 1991 (exam 5). We included 2732 adults aged >= 25 years with complete dietary data in at least three examinations from 1991 to 2008. Descriptive statistics were generated using SAS version 9.3, and a repeated-measures model was used to examine trends in macronutrient and food intakes using R. Over the 17 years of follow-up, the percentage of energy derived from total fat and protein increased (27.3-29.8% of energy and 16.8-18.0% of energy, respectively) and that derived from carbohydrate decreased (51.0-46.8% of energy; P-trend <0.001). Increases in the percentage of energy derived from all fat subtypes were observed, except for that derived from trans-fats, which decreased over time (P-trend <0.001). Trends were similar between the sexes, although women exhibited a greater increase in the percentage of energy derived from saturated fat and less reduction in the percentage of energy derived from trans-fats (P interaction <0.05). Trends in fat intake were similar across the BMI categories. The number of weekly servings of cheese, eggs, ice cream desserts, nuts, butter and sausages/processed meats increased, whereas the intake of milk, margarine, poultry, confectioneries, chips and breads decreased (P-trend <0.001). In this cohort of predominantly Caucasian older adults, the percentage of energy derived from dietary fats increased over time, but it remained within the national recommendations of less than 35% of total energy, on average. C1 [Vadiveloo, Maya; Parekh, Niyati] NYU, Dept Nutr Food Studies & Publ Hlth, New York, NY 10003 USA. [Scott, Marc] NYU, Dept Humanities & Social Sci, New York, NY 10003 USA. [Quatromoni, Paula] Boston Univ, Dept Hlth Sci, Coll Hlth & Rehabil Sci, Sargent Coll, Boston, MA 02215 USA. [Jacques, Paul] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Jacques, Paul] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Parekh, Niyati] NYU, Langone Sch Med, Dept Populat Hlth, New York, NY 10003 USA. RP Parekh, N (reprint author), NYU, Dept Nutr Food Studies & Publ Hlth, 411 Lafayette St,5th Floor, New York, NY 10003 USA. EM niyati.parekh@nyu.edu OI parekh , niyati /0000-0002-1334-0528 FU National Heart, Lung, and Blood Institute; Boston University [N01-HC-25195]; ARS [53-3k06-5-10, 58-1950-9-001, 58-1950-4-401, 58-1950-7-707]; American Cancer Society [RSG-12-005-01-CNE]; New York University's IDEA Research Challenge Grant FX Publicly available data from the FHS were utilised in the present study for secondary analyses. The FHS is conducted and supported by the National Heart, Lung, and Blood Institute in collaboration with Boston University (contract no. N01-HC-25195). Funding support for the Framingham FFQ datasets was provided by ARS contract no. 53-3k06-5-10, ARS agreement no. 58-1950-9-001, 58-1950-4-401 and 58-1950-7-707. This manuscript was not prepared in collaboration with the investigators of the FHS and does not necessarily reflect the opinions or views of the FHS, Boston University or National Heart, Lung, and Blood Institute.; The present study was supported by the American Cancer Society Research Scholar Grant (no. RSG-12-005-01-CNE) and New York University's IDEA Research Challenge Grant awarded to N. P. The American Cancer Society had no role in the design and analysis of the study or in the writing of this article. NR 34 TC 15 Z9 15 U1 3 U2 13 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD FEB 28 PY 2014 VL 111 IS 4 BP 724 EP 734 DI 10.1017/S0007114513002924 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AD0SE UT WOS:000332943600018 PM 24047827 ER PT J AU Glaubitz, JC Casstevens, TM Lu, F Harriman, J Elshire, RJ Sun, Q Buckler, ES AF Glaubitz, Jeffrey C. Casstevens, Terry M. Lu, Fei Harriman, James Elshire, Robert J. Sun, Qi Buckler, Edward S. TI TASSEL-GBS: A High Capacity Genotyping by Sequencing Analysis Pipeline SO PLOS ONE LA English DT Article ID GENOME-WIDE ASSOCIATION; MAPPING POPULATION; ASCERTAINMENT BIAS; GENETIC-VARIATION; CROP IMPROVEMENT; SNP DISCOVERY; MAIZE; IMPUTATION; MARKERS; HISTORY AB Genotyping by sequencing (GBS) is a next generation sequencing based method that takes advantage of reduced representation to enable high throughput genotyping of large numbers of individuals at a large number of SNP markers. The relatively straightforward, robust, and cost-effective GBS protocol is currently being applied in numerous species by a large number of researchers. Herein we describe a bioinformatics pipeline, TASSEL-GBS, designed for the efficient processing of raw GBS sequence data into SNP genotypes. The TASSEL-GBS pipeline successfully fulfills the following key design criteria: (1) Ability to run on the modest computing resources that are typically available to small breeding or ecological research programs, including desktop or laptop machines with only 8-16 GB of RAM, (2) Scalability from small to extremely large studies, where hundreds of thousands or even millions of SNPs can be scored in up to 100,000 individuals (e. g., for large breeding programs or genetic surveys), and (3) Applicability in an accelerated breeding context, requiring rapid turnover from tissue collection to genotypes. Although a reference genome is required, the pipeline can also be run with an unfinished "pseudo-reference" consisting of numerous contigs. We describe the TASSEL-GBS pipeline in detail and benchmark it based upon a large scale, species wide analysis in maize (Zea mays), where the average error rate was reduced to 0.0042 through application of population genetic-based SNP filters. Overall, the GBS assay and the TASSEL-GBS pipeline provide robust tools for studying genomic diversity. C1 [Glaubitz, Jeffrey C.; Casstevens, Terry M.; Lu, Fei; Harriman, James; Elshire, Robert J.; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Sun, Qi] Cornell Univ, Biotechnol Resource Ctr, Bioinformat Facil, Ithaca, NY USA. [Buckler, Edward S.] USDA ARS, Ithaca, NY 14853 USA. RP Glaubitz, JC (reprint author), Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. EM jcg233@cornell.edu OI Buckler, Edward/0000-0002-3100-371X; Elshire, Robert/0000-0003-1753-6920 FU National Science Foundation [DBI-0820619, IOS-1238014, IOS-0965342]; USDA-ARS FX This work was supported by the National Science Foundation (www.nsf.gov) under the Plant Genome Research Program (PGRP) (grant numbers DBI-0820619 and IOS-1238014) and the Basic Research to Enable Agricultural Development (BREAD) project (ID:IOS-0965342), as well as by the USDA-ARS (www.usda.gov). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 173 Z9 175 U1 17 U2 131 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 28 PY 2014 VL 9 IS 2 AR e90346 DI 10.1371/journal.pone.0090346 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AC3DW UT WOS:000332396200176 PM 24587335 ER PT J AU Hwang, S Miller, WG Ryu, S Jeon, B AF Hwang, Sunyoung Miller, William G. Ryu, Sangryeol Jeon, Byeonghwa TI Divergent Distribution of the Sensor Kinase CosS in Non-Thermotolerant Campylobacter Species and Its Functional Incompatibility with the Response Regulator CosR of Campylobacter jejuni SO PLOS ONE LA English DT Article ID 2-COMPONENT SIGNAL-TRANSDUCTION; SYSTEM; COLONIZATION; FAMILY; INFECTIONS; EXPRESSION; APPARATUS; GROWTH; PYLORI AB Two-component signal transduction systems are commonly composed of a sensor histidine kinase and a cognate response regulator, modulating gene expression in response to environmental changes through a phosphorylation-dependent process. CosR is an OmpR-type response regulator essential for the viability of Campylobacter jejuni, a major foodborne pathogenic species causing human gastroenteritis. Although CosR is a response regulator, its cognate sensor kinase has not been identified in C. jejuni. In this study, DNA sequence analysis of the cosR flanking regions revealed that a gene encoding a putative sensor kinase, which we named cosS, is prevalent in non-thermotolerant Campylobacter spp., but not in thermotolerant campylobacters. Phosphorylation assays indicated that C. fetus CosS rapidly autophosphorylates and then phosphorylates C. fetus CosR, suggesting that the CosRS system constitutes a paired two-component signal transduction system in C. fetus. However, C. fetus CosS does not phosphorylate C. jejuni CosR, suggesting that CosR may have different regulatory cascades between thermotolerant and non-thermotolerant Campylobacter species. Comparison of CosR homolog amino acid sequences showed that the conserved phosphorylation residue (D51), which is present in all non-thermotolerant Campylobacter spp., is absent from the CosR homologs of thermotolerant Campylobacter species. However, C. jejuni CosR was not phosphorylated by C. fetus CosS even after site-directed mutagenesis of N51D, implying that C. jejuni CosR may possibly function phosphorylation-independently. In addition, the results of cosS mutational analysis indicated that CosS is not associated with the temperature dependence of the Campylobacter spp. despite its unique divergent distribution only in non-thermotolerant campylobacters. The findings in this study strongly suggest that thermotolerant and non-thermotolerant Campylobacter spp. have different signal sensing mechanisms associated with the CosR regulation. C1 [Jeon, Byeonghwa] Univ Alberta, Sch Publ Hlth, Edmonton, AB, Canada. [Hwang, Sunyoung; Ryu, Sangryeol] Seoul Natl Univ, Dept Agr Biotechnol, Dept Food & Anim Biotechnol, Seoul, South Korea. [Hwang, Sunyoung; Ryu, Sangryeol] Seoul Natl Univ, Ctr Food & Bioconvergence, Seoul, South Korea. [Miller, William G.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. RP Ryu, S (reprint author), Seoul Natl Univ, Dept Agr Biotechnol, Dept Food & Anim Biotechnol, Seoul, South Korea. EM sangryu@snu.ac.kr; bjeon@ualberta.ca FU NSERC [401843-2012-RGPIN]; Basic Science Research Program through the National Research Foundation of Korea (NRF) [NRF-2011-0015091]; Ministry of Education, Science and Technology FX This study is supported by NSERC Discovery Grant 401843-2012-RGPIN to BJ. SH is supported by grant NRF-2011-0015091 from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 2 Z9 2 U1 1 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 28 PY 2014 VL 9 IS 2 AR e89774 DI 10.1371/journal.pone.0089774 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AC3DW UT WOS:000332396200080 PM 24587027 ER PT J AU McGuire, KJ Sebestyen, SD Ohte, N Elliott, EM Gomi, T Green, MB McGlynn, BL Tokuchi, N AF McGuire, Kevin J. Sebestyen, Stephen D. Ohte, Nobuhito Elliott, Emily M. Gomi, Takashi Green, Mark B. McGlynn, Brian L. Tokuchi, Naoko TI Merging perspectives in the catchment sciences: the US-Japan Joint Seminar on catchment hydrology and forest biogeochemistry SO HYDROLOGICAL PROCESSES LA English DT Editorial Material C1 [McGuire, Kevin J.] Virginia Tech, Virginia Water Resources Res Ctr, Blacksburg, VA 24061 USA. [McGuire, Kevin J.] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Sebestyen, Stephen D.] US Forest Serv, No Res Stn, USDA, Grand Rapids, MN 55744 USA. [Ohte, Nobuhito] Univ Tokyo, Dept Forest Sci, Grad Sch Agr & Life Sci, Tokyo 1138657, Japan. [Elliott, Emily M.] Univ Pittsburgh, Dept Geol & Planetary Sci, Pittsburgh, PA 15260 USA. [Gomi, Takashi] Tokyo Univ Agr & Technol, Dept Int Environm & Agr Sci, Tokyo 1138657, Japan. [Green, Mark B.] Plymouth State Univ, Ctr Environm, Plymouth, NH 03264 USA. [Green, Mark B.] US Forest Serv, No Res Stn, USDA, Durham, NH 03824 USA. [McGlynn, Brian L.] Duke Univ, Nicholas Sch Environm, Div Earth & Ocean Sci, Durham, NC 27708 USA. [Tokuchi, Naoko] Kyoto Univ, Field Sci Educ & Res Ctr, Kyoto 6068152, Japan. RP McGuire, KJ (reprint author), Virginia Water Resources Res Ctr MC 0444, Cheatham Hall,Suite 210,310 West Campus Dr, Blacksburg, VA 24061 USA. EM kevin.mcguire@vt.edu RI McGlynn, Brian/A-2509-2008; Sebestyen, Stephen/D-1238-2013; Gomi, Takashi/E-3504-2010; McGuire, Kevin/E-7770-2010; OI McGlynn, Brian/0000-0001-5266-4894; Sebestyen, Stephen/0000-0002-6315-0108; McGuire, Kevin/0000-0001-5751-3956; Green, Mark/0000-0002-7415-7209; Ohte, Nobuhito/0000-0003-0658-6834 NR 10 TC 0 Z9 0 U1 0 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD FEB 28 PY 2014 VL 28 IS 5 BP 2878 EP 2880 DI 10.1002/hyp.10129 PG 3 WC Water Resources SC Water Resources GA AA7CM UT WOS:000331254700014 ER PT J AU Neves, HHR Carvalheiro, R O'Brien, AMP Utsunomiya, YT do Carmo, AS Schenkel, FS Solkner, J McEwan, JC Van Tassell, CP Cole, JB da Silva, MVGB Queiroz, SA Sonstegard, TS Garcia, JF AF Neves, Haroldo H. R. Carvalheiro, Roberto Perez O'Brien, Ana M. Utsunomiya, Yuri T. do Carmo, Adriana S. Schenkel, Flavio S. Soelkner, Johann McEwan, John C. Van Tassell, Curtis P. Cole, John B. da Silva, Marcos V. G. B. Queiroz, Sandra A. Sonstegard, Tad S. Garcia, Jose Fernando TI Accuracy of genomic predictions in Bos indicus (Nellore) cattle SO GENETICS SELECTION EVOLUTION LA English DT Article ID ESTIMATED BREEDING VALUES; DAIRY-CATTLE; LINKAGE DISEQUILIBRIUM; NORDIC HOLSTEIN; BEEF-CATTLE; DATA SETS; SELECTION; INFORMATION; POPULATIONS; SHRINKAGE AB Background: Nellore cattle play an important role in beef production in tropical systems and there is great interest in determining if genomic selection can contribute to accelerate genetic improvement of production and fertility in this breed. We present the first results of the implementation of genomic prediction in a Bos indicus (Nellore) population. Methods: Influential bulls were genotyped with the Illumina Bovine HD chip in order to assess genomic predictive ability for weight and carcass traits, gestation length, scrotal circumference and two selection indices. 685 samples and 320 238 single nucleotide polymorphisms (SNPs) were used in the analyses. A forward-prediction scheme was adopted to predict the genomic breeding values (DGV). In the training step, the estimated breeding values (EBV) of bulls were deregressed (dEBV) and used as pseudo-phenotypes to estimate marker effects using four methods: genomic BLUP with or without a residual polygenic effect (GBLUP20 and GBLUP0, respectively), a mixture model (Bayes C) and Bayesian LASSO (BLASSO). Empirical accuracies of the resulting genomic predictions were assessed based on the correlation between DGV and dEBV for the testing group. Results: Accuracies of genomic predictions ranged from 0.17 (navel at weaning) to 0.74 (finishing precocity). Across traits, Bayesian regression models (Bayes C and BLASSO) were more accurate than GBLUP. The average empirical accuracies were 0.39 (GBLUP0), 0.40 (GBLUP20) and 0.44 (Bayes C and BLASSO). Bayes C and BLASSO tended to produce deflated predictions (i. e. slope of the regression of dEBV on DGV greater than 1). Further analyses suggested that higher-than-expected accuracies were observed for traits for which EBV means differed significantly between two breeding subgroups that were identified in a principal component analysis based on genomic relationships. Conclusions: Bayesian regression models are of interest for future applications of genomic selection in this population, but further improvements are needed to reduce deflation of their predictions. Recurrent updates of the training population would be required to enable accurate prediction of the genetic merit of young animals. The technical feasibility of applying genomic prediction in a Bos indicus (Nellore) population was demonstrated. Further research is needed to permit cost-effective selection decisions using genomic information. C1 [Neves, Haroldo H. R.; Carvalheiro, Roberto; Utsunomiya, Yuri T.; do Carmo, Adriana S.; Queiroz, Sandra A.] Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, BR-14884900 Sao Paulo, Brazil. [Carvalheiro, Roberto] GenSys Consultores Assoc SC Ltda, BR-90680000 Porto Alegre, RS, Brazil. [Perez O'Brien, Ana M.; Soelkner, Johann] Univ Nat Resources & Life Sci, Div Livestock Sci, Dept Sustainable Agr Syst BOKU, A-1180 Vienna, Austria. [Schenkel, Flavio S.] Univ Guelph, Ctr Genet Improvement Livestock, Guelph, ON N1G 2W1, Canada. [McEwan, John C.] AgResearch, Ctr Reprod & Genom, Invermay, Mosgiel, New Zealand. [Van Tassell, Curtis P.; Sonstegard, Tad S.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. [Cole, John B.] ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. [da Silva, Marcos V. G. B.] Embrapa DairyCattle, Bioinformat & Anim Genom Lab, Juiz De Fora, MG, Brazil. [Garcia, Jose Fernando] Univ Estadual Paulista, UNESP, Fac Med Vet Aracatuba, BR-16050680 Sao Paulo, Brazil. RP Neves, HHR (reprint author), Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, BR-14884900 Sao Paulo, Brazil. EM haroldozoo@hotmail.com RI de Rezende Neves, Haroldo Henrique/F-9521-2012; Utsunomiya, Yuri Tani/K-5214-2013; Queiroz, Sandra/J-8150-2012; Cole, John/J-8571-2014; McEwan, John/A-1143-2008; OI Utsunomiya, Yuri Tani/0000-0002-6526-8337; Cole, John/0000-0003-1242-4401; McEwan, John/0000-0003-4801-6207; Carvalheiro, Roberto/0000-0002-4506-0555; Van Tassell, Curtis/0000-0002-8416-2087 FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [560922/2010-8, 483590/2010-0]; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010/06185-4, 2011/16643-2, 2010/52030-2]; (BFGL) from the USDA Agricultural Research Service [1265-31000-104D] FX The authors acknowledge DeltaGen and GenSys for providing the data. We thank the developers of gebv and GS3 for making available the software used in data analyses. We thank Guilherme Penteado Coelho Filho and Daniel Biluca for technical assistance in sample acquisition and constructive discussions. We thank Alicia Bertles (USDA, BFGL) for training in processing of genotyping reactions. We thank the editor and two anonymous reviewers for their constructive comments. This research was supported by: Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)-processes: 560922/2010-8 and 483590/2010-0; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)-processes: 2010/06185-4, 2011/16643-2 and 2010/52030-2. This project was also supported by the project 1265-31000-104D (BFGL) from the USDA Agricultural Research Service. NR 48 TC 16 Z9 16 U1 2 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0999-193X EI 1297-9686 J9 GENET SEL EVOL JI Genet. Sel. Evol. PD FEB 27 PY 2014 VL 46 AR 17 DI 10.1186/1297-9686-46-17 PG 13 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA AD8LQ UT WOS:000333517900001 PM 24575732 ER PT J AU Meinhardt, LW Costa, GGL Thomazella, DPT Teixeira, PJPL Carazzolle, MF Schuster, SC Carlson, JE Guiltinan, MJ Mieczkowski, P Farmer, A Ramaraj, T Crozier, J Davis, RE Shao, J Melnick, RL Pereira, GAG Bailey, BA AF Meinhardt, Lyndel W. Lacerda Costa, Gustavo Gilson Thomazella, Daniela P. T. Teixeira, Paulo Jose P. L. Carazzolle, Marcelo Falsarella Schuster, Stephan C. Carlson, John E. Guiltinan, Mark J. Mieczkowski, Piotr Farmer, Andrew Ramaraj, Thiruvarangan Crozier, Jayne Davis, Robert E. Shao, Jonathan Melnick, Rachel L. Pereira, Goncalo A. G. Bailey, Bryan A. TI Genome and secretome analysis of the hemibiotrophic fungal pathogen, Moniliophthoraroreri, which causes frosty pod rot disease of cacao: mechanisms of the biotrophic and necrotrophic phases SO BMC GENOMICS LA English DT Article ID WITCHES-BROOM DISEASE; THEOBROMA-CACAO; CERATO-PLATANIN; MAGNAPORTHE-GRISEA; SACCHAROMYCES-CEREVISIAE; CARBOHYDRATE-BINDING; NEP1-LIKE PROTEINS; CRYSTAL-STRUCTURE; GENE-EXPRESSION; CELL-WALLS AB Background: The basidiomycete Moniliophthora roreri is the causal agent of Frosty pod rot (FPR) disease of cacao (Theobroma cacao), the source of chocolate, and FPR is one of the most destructive diseases of this important perennial crop in the Americas. This hemibiotroph infects only cacao pods and has an extended biotrophic phase lasting up to sixty days, culminating in plant necrosis and sporulation of the fungus without the formation of a basidiocarp. Results: We sequenced and assembled 52.3 Mb into 3,298 contigs that represent the M. roreri genome. Of the 17,920 predicted open reading frames (OFRs), 13,760 were validated by RNA-Seq. Using read count data from RNA sequencing of cacao pods at 30 and 60 days post infection, differential gene expression was estimated for the biotrophic and necrotrophic phases of this plant-pathogen interaction. The sequencing data were used to develop a genome based secretome for the infected pods. Of the 1,535 genes encoding putative secreted proteins, 1,355 were expressed in the biotrophic and necrotrophic phases. Analysis of the data revealed secretome gene expression that correlated with infection and intercellular growth in the biotrophic phase and invasive growth and plant cellular death in the necrotrophic phase. Conclusions: Genome sequencing and RNA-Seq was used to determine and validate the Moniliophthora roreri genome and secretome. High sequence identity between Moniliophthora roreri genes and Moniliophthora perniciosa genes supports the taxonomic relationship with Moniliophthora perniciosa and the relatedness of this fungus to other basidiomycetes. Analysis of RNA-Seq data from infected plant tissues revealed differentially expressed genes in the biotrophic and necrotrophic phases. The secreted protein genes that were upregulated in the biotrophic phase are primarily associated with breakdown of the intercellular matrix and modification of the fungal mycelia, possibly to mask the fungus from plant defenses. Based on the transcriptome data, the upregulated secreted proteins in the necrotrophic phase are hypothesized to be actively attacking the plant cell walls and plant cellular components resulting in necrosis. These genes are being used to develop a new understanding of how this disease interaction progresses and to identify potential targets to reduce the impact of this devastating disease. C1 [Meinhardt, Lyndel W.; Melnick, Rachel L.; Bailey, Bryan A.] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD 20705 USA. [Lacerda Costa, Gustavo Gilson; Carazzolle, Marcelo Falsarella] Univ Estadual Campinas, Ctr Nacl Proc Alto Desempenho Sao Paulo, BR-13083970 Campinas, SP, Brazil. [Thomazella, Daniela P. T.; Teixeira, Paulo Jose P. L.; Pereira, Goncalo A. G.] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Genet Evolucao & Bioagentes,Lab Genom & Expr, BR-13083970 Campinas, SP, Brazil. [Schuster, Stephan C.] Penn State Univ, Ctr Infect Dis Dynam, University Pk, PA 16802 USA. [Carlson, John E.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Guiltinan, Mark J.] Penn State Univ, Dept Hort, University Pk, PA 16802 USA. [Mieczkowski, Piotr] Univ N Carolina, Sch Med, Dept Genet, Chapel Hill, NC 27599 USA. [Farmer, Andrew; Ramaraj, Thiruvarangan] Natl Ctr Genom Res, Santa Fe, NM 87505 USA. [Crozier, Jayne] CABI Biosci UK Ctr, Egham, Surrey, England. [Davis, Robert E.; Shao, Jonathan] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. RP Meinhardt, LW (reprint author), ARS, Sustainable Perennial Crops Lab, USDA, Bldg 001 Rm 223 Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. EM lyndel.meinhardt@ars.usda.gov RI Pereira, Goncalo /B-7944-2012; Carazzolle, Marcelo Falsarella/C-6503-2012; Costa, Gustavo/B-6312-2009; UNICAMP, CCES - /J-7787-2015 FU USDA ARS; Center for Computational Engineering and Sciences at UNICAMP SP Brazil (FAPESP/CEPID project) [2013/08293-7] FX This work was funded by USDA ARS. We would like to acknowledge the contributions of the Center for Computational Engineering and Sciences at UNICAMP SP Brazil (FAPESP/CEPID project # 2013/08293-7). References to a company and/or product by the USDA are only for the purposes of information and do not imply approval or recommendation of the product to the exclusion of others that may also be suitable. The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250- 9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 97 TC 20 Z9 23 U1 6 U2 50 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 27 PY 2014 VL 15 AR 164 DI 10.1186/1471-2164-15-164 PG 25 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AC6AZ UT WOS:000332604300001 PM 24571091 ER PT J AU del Cacho, E Gallego, M Lillehoj, HS Quilez, J Lillehoj, EP Ramo, A Sanchez-Acedo, C AF del Cacho, Emilio Gallego, Margarita Lillehoj, Hyun Soon Quilez, Joaquin Lillehoj, Erik P. Ramo, Ana Sanchez-Acedo, Caridad TI IL-17A regulates Eimeria tenella schizont maturation and migration in avian coccidiosis SO VETERINARY RESEARCH LA English DT Article ID HOST-DEFENSE; IMMUNE-RESPONSES; GENE-EXPRESSION; DENDRITIC CELLS; PROTEIN S100A4; INFECTION; METASTASIS; INVASION; BLOOD; IMMUNOPATHOLOGY AB Although IL17A is associated with the immunological control of various infectious diseases, its role in host response to Eimeria infections is not well understood. In an effort to better dissect the role of IL17A in host-pathogen interactions in avian coccidiosis, a neutralizing antibody (Ab) to chicken IL17A was used to counteract IL17A bioactivity in vivo. Chickens infected with Eimeria tenella and treated intravenously with IL17A Ab, exhibited reduced intracellular schizont and merozoite development, diminished lesion score, compared with untreated controls. Immunohistological evaluation of cecal lesions in the parasitized tissues indicated reduced migration and maturation of second-generation schizonts and reduced lesions in lamina propria and submucosa. In contrast, untreated and infected chickens had epithelial cells harboring second-generation schizonts, which extend into the submucosa through muscularis mucosa disruptions, maturing into second generation merozoites. Furthermore, IL17A Ab treatment was associated with increased parameters of Th1 immunity (IL2- and IFN gamma-producing cells), reduced levels of reactive oxygen species (ROS), and diminished levels of serum matrix metalloproteinase-9 (MMP-9). Finally, schizonts from untreated and infected chickens expressed S100, Wiskott-Aldrich syndrome protein family member 3 (WASF3), and heat shock protein-70 (HSP70) proteins as merozoites matured, whereas the expression of these proteins was absent in IL17A Ab-treated chickens. These results provide the first evidence that the administration of an IL17A neutralizing Ab to E. tenella-infected chickens inhibits the migration of parasitized epithelial cells, markedly reduces the production of ROS and MMP-9, and decreases cecal lesions, suggesting that IL17A might be a potential therapeutic target for coccidiosis control. C1 [del Cacho, Emilio; Gallego, Margarita; Quilez, Joaquin; Ramo, Ana; Sanchez-Acedo, Caridad] Univ Zaragoza, Fac Vet Sci, Dept Anim Pathol, Zaragoza, Spain. [Lillehoj, Hyun Soon] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Lillehoj, Erik P.] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA. RP del Cacho, E (reprint author), Univ Zaragoza, Fac Vet Sci, Dept Anim Pathol, Zaragoza, Spain. EM edelcach@unizar.es OI Ramo, Ana/0000-0002-0308-1390 FU Research Council of Aragon (Spain) [A46]; USDA-CSREES [proposal 2005-01812] FX This investigation was partially funded by Grant A46 from the Research Council of Aragon (Spain) and USDA-CSREES proposal 2005-01812 (US Veterinary Immune Reagent Network). NR 41 TC 2 Z9 2 U1 2 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD FEB 26 PY 2014 VL 45 AR 25 DI 10.1186/1297-9716-45-25 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA AF3KX UT WOS:000334612200001 PM 24571471 ER PT J AU Buffum, B Modisette, C McWilliams, SR AF Buffum, Bill Modisette, Christopher McWilliams, Scott R. TI Encouraging Family Forest Owners to Create Early Successional Wildlife Habitat in Southern New England SO PLOS ONE LA English DT Article ID NORTHEASTERN UNITED-STATES; PRIVATE LANDS; PROGRAMS; MANAGEMENT AB Encouraging family forest owners to create early successional habitat is a high priority for wildlife conservation agencies in the northeastern USA, where most forest land is privately owned. Many studies have linked regional declines in wildlife populations to the loss of early successional habitat. The government provides financial incentives to create early successional habitat, but the number of family forest owners who actively manage their forests remains low. Several studies have analyzed participation of family forest owners in federal forestry programs, but no study to date has focused specifically on creation of wildlife habitat. The objective of our study was to analyze the experience of a group of wildlife-oriented family forest owners who were trained to create early successional habitat. This type of family forest owners represents a small portion of the total population of family forest owners, but we believe they can play an important role in creating wildlife habitat, so it is important to understand how outreach programs can best reach them. The respondents shared some characteristics but differed in terms of forest holdings, forestry experience and interest in earning forestry income. Despite their strong interest in wildlife, awareness about the importance of early successional habitat was low. Financial support from the federal government appeared to be important in motivating respondents to follow up after the training with activities on their own properties: 84% of respondents who had implemented activities received federal financial support and 47% would not have implemented the activities without financial assistance. In order to mobilize greater numbers of wildlife-oriented family forest owners to create early successional habitat we recommend focusing outreach efforts on increasing awareness about the importance of early successional habitat and the availability of technical and financial assistance. C1 [Buffum, Bill] Univ Rhode Isl, Dept Nat Resources Management, Kingston, RI 02881 USA. [Modisette, Christopher] Nat Resources Conservat Serv, USDA, Warwick, RI USA. [McWilliams, Scott R.] Univ Rhode Isl, Dept Nat Resources Management, Kingston, RI 02881 USA. RP Buffum, B (reprint author), Univ Rhode Isl, Dept Nat Resources Management, Kingston, RI 02881 USA. EM buffum@uri.edu FU URI; United States Department of Agriculture through a McIntire-Stennis Cooperative Research Grant [RIAES-MS975] FX This research was conducted as part of a University of Rhode Island (URI) project entitled "Forest Management for Wildlife Habitat in Rhode Island" with funding from URI and the United States Department of Agriculture through a McIntire-Stennis Cooperative Research Grant (RIAES-MS975). This is contribution #5354 of the University of Rhode Island Agricultural Experiment Station. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 25 TC 1 Z9 1 U1 1 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 26 PY 2014 VL 9 IS 2 AR e89972 DI 10.1371/journal.pone.0089972 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AC3BC UT WOS:000332389000123 PM 24587160 ER PT J AU Uchimiya, M Hiradate, S AF Uchimiya, Minori Hiradate, Syuntaro TI Pyrolysis Temperature-Dependent Changes in Dissolved Phosphorus Speciation of Plant and Manure Biochars SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE organic fertilizer; remediation; charcoal; bioenergy; biomass ID MAGNETIC-RESONANCE-SPECTROSCOPY; BROILER LITTER BIOCHARS; P-31 NMR-SPECTROSCOPY; POULTRY LITTER; ORGANIC PHOSPHORUS; AQUEOUS-SOLUTIONS; PHYTIC ACID; SOIL; PHOSPHATE; RELEASE AB Pyrolysis of plant and animal wastes produces a complex mixture of phosphorus species in amorphous, semicrystalline, and crystalline inorganic phases, organic (char) components, and within organo-mineral complexes. To understand the solubility of different phosphorus species, plant (cottonseed hull) and manure (broiler litter) wastes were pyrolyzed at 350, 500, 650, and 800 degrees C and exposed to increasingly more rigorous extraction procedures: water (16 h), Mehlich 3 (1 mM EDTA at pH 2.5 for 5 mm), oxalate (200 mM oxalate at pH 3.5 for 4 h), NaOH-EDTA (250 mM NaOH + 5 mM EDTA for 16 h), and total by microwave digestion (concentrated HNO3/HCl + 30% H2O2). Relative to the total (microwave digestible) P, the percentage of extractable P increased in the following order: M3 < oxalate approximate to water < NaOH-EDTA for plant biochars and water < M3 < NaOH-EDTA < oxalate for manure biochars. Solution phase P-31 NMR analysis of NaOH-EDTA extracts showed the conversion of phytate to inorganic P by pyrolysis of manure and plant wastes at 350 degrees C. Inorganic orthophosphate (PO43-) became the sole species of >= 500 degrees C manure biochars, whereas pyrophosphate (P2O74-) persisted in plant biochars up to 650 degrees C. These observations suggested the predominance of (i) amorphous (rather than crystalline) calcium phosphate in manure biochars, especially at >= 650 degrees C, and (ii) strongly complexed pyrophosphate in plant biochars (especially at 350-500 degrees C). Correlation (Pearson's) was observed (i) between electric conductivity and ash content of biochars with the amount of inorganic P species and (ii) between total organic carbon and volatile matter contents with the organic P species. C1 [Uchimiya, Minori] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Hiradate, Syuntaro] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan. RP Uchimiya, M (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM sophie.uchimiya@ars.usda.gov NR 57 TC 19 Z9 19 U1 12 U2 79 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 26 PY 2014 VL 62 IS 8 BP 1802 EP 1809 DI 10.1021/jf4053385 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AB9VS UT WOS:000332144400008 PM 24495088 ER PT J AU Zeller, WE AF Zeller, Wayne E. TI Synthesis of 1-O-Methylchlorogenic Acid: Reassignment of Structure for MCGA3 Isolated from Bamboo (Phyllostachys edulis) Leaves SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE 1-O-methylchlorogenic acid; MCGA3; Phyllostachys edulis; methyl chlorogenate ID CHLOROGENIC ACID; IN-VITRO; CAFFEIC ACID; 3-O-CAFFEOYL-1-METHYLQUINIC ACID; ANTIOXIDANT ACTIVITIES; GLUCOSE-6-PHOSPHATE TRANSLOCASE; PHENOLIC-COMPOUNDS; HIV-1 REPLICATION; DERIVATIVES; INHIBITORS AB The first synthesis of 1-O-methylchlorogenic acid is described. The short and efficient synthesis of this compound provides laboratory-scale quantities of the material to investigate its biological properties. The synthesis involves C-1 alkylation of the known (-)-4,5-cyclohexylidenequinic acid lactone followed by methoxide opening to the hydroxyl ester. Acylation of the C-5 hydroxyl group followed by sequential removal of protecting groups afforded 1-O-methylchlorogenic acid. The NMR spectroscopic characteristics of this compound do not coincide with those reported for the original isolation from bamboo (Phyllostachys edulis) leaves of the compound designated MCGA3. Comparison of the published spectroscopic data reported for MCGA3, with both reported literature values and spectroscopic data obtained from an authentic sample, leads to the conclusion that the compound isolated from bamboo (Phyllostachys edulis) leaves is instead methyl chlorogenate. C1 ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA. RP Zeller, WE (reprint author), ARS, US Dairy Forage Res Ctr, USDA, 1925 Linden Dr, Madison, WI 53706 USA. EM Wayne.Zeller@ars.usda.gov FU NSF [CHE9974839] FX Purchase of the Waters LCT instrument in 2000 was partially funded by NSF award no. CHE9974839 to the Department of Chemistry, University of Wisconsin-Madison. NR 43 TC 1 Z9 1 U1 1 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 26 PY 2014 VL 62 IS 8 BP 1860 EP 1865 DI 10.1021/jf4042112 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AB9VS UT WOS:000332144400016 PM 24460043 ER PT J AU Kim, H Bartley, GE Arvik, T Lipson, R Nah, SY Seo, K Yokoyama, W AF Kim, Hyunsook Bartley, Glenn E. Arvik, Torey Lipson, Rebecca Nah, Seung-Yeol Seo, Kunho Yokoyama, Wallace TI Dietary Supplementation of Chardonnay Grape Seed Flour Reduces Plasma Cholesterol Concentration, Hepatic Steatosis, and Abdominal Fat Content in High-Fat Diet-Induced Obese Hamsters SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE cholesterol; antiobesity; grape seed flour; bile acid; inflammation ID OXIDATIVE STRESS; GENE-EXPRESSION; HYDROXYPROPYL METHYLCELLULOSE; (-)-EPIGALLOCATECHIN GALLATE; LOWERING ACTIVITY; 3T3-L1 CELLS; HEPG2 CELLS; EXTRACT; LIVER; PROCYANIDINS AB The mechanisms for the hypocholesterolemic and antiobesity effects of grape seed flours derived from white and red winemaking processing were investigated using male Golden Syrian hamsters fed high-fat (HF) diets supplemented with 10% partially defatted grape seed flours from Chardonnay (ChrSd), Cabernet Sauvignon (CabSd), or Syrah (SyrSd) pomace as compared to a HF control diet for 3 weeks. Hamsters fed the ChrSd diet had significantly lowered plasma total-, VLDL-, and LDL-cholesterol concentrations compared to the CabSd, SyrSd, and control diets. The improved plasma cholesterol after ChrSd was correlated with the up-regulation of hepatic genes related to cholesterol (CYP51) and bile acid (CYP7A1) synthesis as well as LDL-cholesterol uptake (LDLR). A reduction of hepatic lipid content was associated with altered expression of the genes related to lipid metabolism. However, fecal total lipid content was not changed. Expression of ileal apical sodium bile acid transporter (ASBT) was not affected by ChrSd, indicating unchanged ileal bile acid reabsorption. The antiobesity effect of the ChrSd diet appears to be related to expression of adipogenesis- and inflammation-related genes in adipose tissue. These findings suggest that flavonoid-rich Chardonnay grape seed flour induced cholesterol-lowering, antiobesity, and anti-inflammatory health benefits and attenuation of hepatic steatosis via regulation of gene expression related to cholesterol, bile acid, and lipid metabolism in liver and adipose tissue. C1 [Kim, Hyunsook] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Bartley, Glenn E.; Yokoyama, Wallace] ARS, USDA, Albany, CA 94710 USA. [Kim, Hyunsook; Arvik, Torey; Lipson, Rebecca] Sonomaceuticals LLC, WholeVine Prod, Santa Rosa, CA 95403 USA. [Kim, Hyunsook; Nah, Seung-Yeol] Konkuk Univ, Coll Vet Med, Dept Physiol, Seoul 143701, South Korea. [Seo, Kunho] Konkuk Univ, Coll Vet Med, KU Ctr Food Safety, Seoul 143701, South Korea. RP Kim, H (reprint author), Konkuk Univ, Coll Vet Med, Dept Physiol, Seoul 143701, South Korea. EM hyunsk13@konkuk.ac.kr; Wally.Yokoyama@ars.usda.edu FU KU Research Professor Program of Konkuk University FX H.K. was supported by the KU Research Professor Program of Konkuk University. NR 32 TC 8 Z9 8 U1 0 U2 19 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 26 PY 2014 VL 62 IS 8 BP 1919 EP 1925 DI 10.1021/jf404832s PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AB9VS UT WOS:000332144400024 PM 24517872 ER PT J AU Silva-Sanchez, C Chen, SX Li, JX Chourey, PS AF Silva-Sanchez, Cecilia Chen, Sixue Li, Jinxi Chourey, Prem S. TI A comparative glycoproteome study of developing endosperm in the hexose-deficient miniature1 (mn1) seed mutant and its wild type Mn1 in maize SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE seed development; gene expression; sugar methabolism; transfer cells; maize ID CELL-WALL INVERTASE; UDP-GLUCOSE PYROPHOSPHORYLASE; GERMIN-LIKE PROTEINS; GLYCOSYLATED PROTEINS; GLUTAMINE-SYNTHETASE; STRESS RESPONSES; PICHIA-PASTORIS; QUALITY-CONTROL; IDENTIFICATION; PLANTS AB In maize developing seeds, transfer cells are prominently located at the basal endosperm transfer layer (BETL). As the first filial cell layer, BETL is a gateway to sugars, nutrients and water from mother plant; and anchor of numerous functions such as sucrose turnover, auxin and cytokinin biosynthesis/accumulation, energy metabolism, defense response, and signaling between maternal and filial generations. Previous studies showed that basal developing endosperms of miniature1 (mn1) mutant seeds lacking the Mn1-encoded cell wall invertase II, are also deficient for hexose. Given the role of glucose as one of the key sugars in protein glycosylation and proper protein folding; we performed a comparative large scale glycoproteome profiling of total proteins of these two genotypes (mn1 mutant vs. Mn1 wild type) using 2D gel electrophoresis and glycosylation/total protein staining, followed by image analysis. Protein identification was done by LC-MS/MS. A total of 413 spots were detected; from which, 113 spots matched between the two genotypes. Of these, 45 showed >20% decrease/increase in glycosylation level and were selected for protein identification. A large number of identified proteins showed decreased glycosylation levels in mn1 developing endosperms as compared to the Mn1. Functional classification of proteins, showed mainly of post-translational modification, protein turnover, chaperone activities, carbohydrate and amino acid biosynthesis/transport, and cell wall biosynthesis. These proteins and activities were related to endoplasmic reticulum (ER) stress and unfolded protein response (UPR) as a result of the low glycolsylation levels of the mutant proteins. Overall, these results provide for the first time a global glycoproteome profile of maize BETL-enriched basal endosperm to better understand their role in seed development in maize. C1 [Silva-Sanchez, Cecilia; Chen, Sixue; Li, Jinxi] Univ Florida, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL USA. [Chen, Sixue] Univ Florida, UF Genet Inst, Dept Biol, Gainesville, FL USA. [Chourey, Prem S.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Chourey, Prem S.] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. RP Chourey, PS (reprint author), ARS, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM prem.chourey@ars.usda.gov NR 71 TC 2 Z9 2 U1 1 U2 20 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 26 PY 2014 VL 5 AR 63 DI 10.3389/fpls.2014.00063 PG 14 WC Plant Sciences SC Plant Sciences GA AB6ZA UT WOS:000331937700001 PM 24616729 ER PT J AU Mech, LD Christensen, BW Asa, CS Callahan, M Young, JK AF Mech, L. David Christensen, Bruce W. Asa, Cheryl S. Callahan, Margaret Young, Julie K. TI Production of Hybrids between Western Gray Wolves and Western Coyotes SO PLOS ONE LA English DT Article ID GENETIC-CHARACTERIZATION; EVOLUTIONARY HISTORY; FERTILE ESTRUS; NORTH-AMERICA; GNRH-AGONIST; CANIS-LUPUS; WOLF; EASTERN; HYBRIDIZATION; PREGNANCY AB Using artificial insemination we attempted to produce hybrids between captive, male, western, gray wolves (Canis lupus) and female, western coyotes (Canis latrans) to determine whether their gametes would be compatible and the coyotes could produce and nurture offspring. The results contribute new information to an ongoing controversy over whether the eastern wolf (Canis lycaon) is a valid unique species that could be subject to the U.S. Endangered Species Act. Attempts with transcervically deposited wolf semen into nine coyotes over two breeding seasons yielded three coyote pregnancies. One coyote ate her pups, another produced a resorbed fetus and a dead fetus by C-section, and the third produced seven hybrids, six of which survived. These results show that, although it might be unlikely for male western wolves to successfully produce offspring with female western coyotes under natural conditions, western-gray-wolf sperm are compatible with western-coyote ova and that at least one coyote could produce and nurture hybrid offspring. This finding in turn demonstrates that gamete incompatibility would not have prevented western, gray wolves from inseminating western coyotes and thus producing hybrids with coyote mtDNA, a claim that counters the view that the eastern wolf is a separate species. However, some of the difficulties experienced by the other inseminated coyotes tend to temper that finding and suggest that more experimentation is needed, including determining the behavioral and physical compatibility of western gray wolves copulating with western coyotes. Thus although our study adds new information to the controversy, it does not settle it. Further study is needed to determine whether the putative Canis lycaon is indeed a unique species. C1 [Mech, L. David] US Geol Survey, Northern Prairie Wildlife Res Ctr, Jamestown, ND USA. [Christensen, Bruce W.] Univ Calif Davis, Sch Vet Med, Dept Populat Hlth & Reprod, Davis, CA 95616 USA. [Asa, Cheryl S.] St Louis Zoo, Res Dept, St Louis, MO USA. [Callahan, Margaret] Wildlife Sci Ctr, Forest Lake, MN USA. [Young, Julie K.] Utah State Univ, Dept Wildland Resources, Natl Wildlife Res Ctr, USDA,Wildlife Serv, Logan, UT 84322 USA. RP Mech, LD (reprint author), US Geol Survey, Northern Prairie Wildlife Res Ctr, Jamestown, ND USA. EM mechx002@umn.edu FU Northern Prairie Wildlife Research Center, U.S.Geological Survey; U.S. Department of Agriculture Wildlife Services - National Wildlife Research Center; Wildlife Science Center; Saint Louis Zoo FX This study was supported by the Northern Prairie Wildlife Research Center, U.S.Geological Survey (http://www.npwrc.usgs.gov/); the U.S. Department of Agriculture Wildlife Services - National Wildlife Research Center (http://www.aphis.usda.gov/wildlife_damage/nwrc/); the Wildlife Science Center (http://www.wildlifesciencecenter.org/), and the Saint Louis Zoo (http://www.stlzoo.org/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 34 TC 9 Z9 9 U1 5 U2 76 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 25 PY 2014 VL 9 IS 2 AR e88861 DI 10.1371/journal.pone.0088861 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AC2ZX UT WOS:000332385900015 PM 24586418 ER PT J AU Norton, GJ Douglas, A Lahner, B Yakubova, E Guerinot, ML Pinson, SRM Tarpley, L Eizenga, GC McGrath, SP Zhao, FJ Islam, MR Islam, S Duan, GL Zhu, YG Salt, DE Meharg, AA Price, AH AF Norton, Gareth J. Douglas, Alex Lahner, Brett Yakubova, Elena Guerinot, Mary Lou Pinson, Shannon R. M. Tarpley, Lee Eizenga, Georgia C. McGrath, Steve P. Zhao, Fang-Jie Islam, M. Rafiqul Islam, Shofiqul Duan, Guilan Zhu, Yongguan Salt, David E. Meharg, Andrew A. Price, Adam H. TI Genome Wide Association Mapping of Grain Arsenic, Copper, Molybdenum and Zinc in Rice (Oryza sativa L.) Grown at Four International Field Sites SO PLOS ONE LA English DT Article ID QUANTITATIVE TRAIT LOCI; ARABIDOPSIS-THALIANA ACCESSIONS; MOLYBDATE TRANSPORTER; BANGLADESH; ACCUMULATION; IDENTIFICATION; CULTIVARS; GENOTYPE; REVEALS; CROPS AB The mineral concentrations in cereals are important for human health, especially for individuals who consume a cereal subsistence diet. A number of elements, such as zinc, are required within the diet, while some elements are toxic to humans, for example arsenic. In this study we carry out genome-wide association (GWA) mapping of grain concentrations of arsenic, copper, molybdenum and zinc in brown rice using an established rice diversity panel of,300 accessions and 36.9 k single nucleotide polymorphisms (SNPs). The study was performed across five environments: one field site in Bangladesh, one in China and two in the US, with one of the US sites repeated over two years. GWA mapping on the whole dataset and on separate subpopulations of rice revealed a large number of loci significantly associated with variation in grain arsenic, copper, molybdenum and zinc. Seventeen of these loci were detected in data obtained from grain cultivated in more than one field location, and six co-localise with previously identified quantitative trait loci. Additionally, a number of candidate genes for the uptake or transport of these elements were located near significantly associated SNPs (within 200 kb, the estimated global linkage disequilibrium previously employed in this rice panel). This analysis highlights a number of genomic regions and candidate genes for further analysis as well as the challenges faced when mapping environmentally-variable traits in a highly genetically structured diversity panel. C1 [Norton, Gareth J.; Douglas, Alex; Salt, David E.; Price, Adam H.] Univ Aberdeen, Inst Biol & Environm Sci, Aberdeen, Scotland. [Lahner, Brett; Yakubova, Elena] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA. [Guerinot, Mary Lou] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. [Pinson, Shannon R. M.; Eizenga, Georgia C.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Tarpley, Lee] Texas A&M Univ Syst, Texas A&M AgriLife Res, Beaumont, TX USA. [McGrath, Steve P.; Zhao, Fang-Jie] Rothamsted Res, Harpenden, Herts, England. [Zhao, Fang-Jie] Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing, Jiangsu, Peoples R China. [Islam, M. Rafiqul; Islam, Shofiqul] Bangladesh Agr Univ, Dept Soil Sci, Mymensingh, Bangladesh. [Duan, Guilan; Zhu, Yongguan] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing, Peoples R China. [Meharg, Andrew A.] Queens Univ Belfast, Inst Global Food Secur, Belfast, Antrim, North Ireland. RP Price, AH (reprint author), Univ Aberdeen, Inst Biol & Environm Sci, Aberdeen, Scotland. EM a.price@abdn.ac.uk RI Meharg, Andrew/F-8182-2014; Norton, Gareth/D-7512-2012; Zhu, Yong-Guan/A-1412-2009; McGrath, Steve/B-5127-2008; Zhao, Fang-Jie/A-8339-2008; OI Meharg, Andrew/0000-0003-2019-0449; Zhu, Yong-Guan/0000-0003-3861-8482; McGrath, Steve/0000-0003-0952-8947; Zhao, Fang-Jie/0000-0002-0164-169X; Norton, Gareth/0000-0003-4560-170X FU BBSRC-DFID [BBF0040871]; U.S. National Science Foundation, Plant Genome Research Program [IOS 0701119, PRGP 0606461]; U.S. National Institute of Environmental Health Sciences [P42ES007373] FX This work was funded by BBSRC-DFID grant BBF0040871, the U.S. National Science Foundation, Plant Genome Research Program (grant #IOS 0701119). Research conducted in Arkansas was supported in part by the U.S. National Science Foundation, Plant Genome Research Program (grant #PRGP 0606461) and research at Dartmouth College was supported by the U.S. National Institute of Environmental Health Sciences (grant P42ES007373). The plant material was imported into the UK under import licence IMP/SOIL/18/2009 issued by Science and Advice for Scottish Agriculture. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture or Texas A&M AgriLife Research, and does not imply its approval to the exclusion of other products that also can be suitable. USDA is an equal opportunity provider and employer. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 69 TC 29 Z9 31 U1 3 U2 86 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 25 PY 2014 VL 9 IS 2 AR e89685 DI 10.1371/journal.pone.0089685 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AC2ZX UT WOS:000332385900067 PM 24586963 ER PT J AU Fajardo, D Schlautman, B Steffan, S Polashock, J Vorsa, N Zalapa, J AF Fajardo, Diego Schlautman, Brandon Steffan, Shawn Polashock, James Vorsa, Nicholi Zalapa, Juan TI The American cranberry mitochondrial genome reveals the presence of selenocysteine (tRNA-Sec and SECIS) insertion machinery in land plants SO GENE LA English DT Article DE Mitochondrial genome; Ericales; Ericaceae; Vacdnium; Cranberry; tRNA-Sec ID GROUP-II INTRONS; EVOLUTION; SEQUENCE; SELENOPROTEINS; ORGANIZATION; CYTOPLASM; GENES; MAIZE AB This is the first de novo assembly and annotation of a complete mitochondrial genome in the Ericales order from the American cranberry (Vaccinium macrocarpon Ait.). Moreover, only four complete Asterid mitochondrial genomes have been made publicly available. The cranberry mitochondrial genome was assembled and reconstructed from whole genome 454 Roche GS-FIX and Illumina shotgun sequences. Compared with other Asterids, the reconstruction of the genome revealed an average size mitochondrion (459,678 nt) with relatively little repetitive sequences and DNA of plastid origin. The complete mitochondrial genome of cranberry was annotated obtaining a total of 34 genes classified based on their putative function, plus three ribosomal RNAs, and 17 transfer RNAs. Maternal organellar cranberry inheritance was inferred by analyzing gene variation in the cranberry mitochondria and plastid genomes. The annotation of cranberry mitochondrial genome revealed the presence of two copies of tRNA-Sec and a selenocysteine insertion sequence (SECIS) element which were lost in plants during evolution. This is the first report of a land plant possessing selenocysteine insertion machinery at the sequence level. Published by Elsevier B.V. C1 [Steffan, Shawn; Zalapa, Juan] USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. [Fajardo, Diego; Schlautman, Brandon] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Polashock, James] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Chatsworth, NJ USA. [Vorsa, Nicholi] Rutgers State Univ, Blueberry & Cranberry Res & Extens Ctr, Chatsworth, NJ USA. RP Zalapa, J (reprint author), USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM jezalapa@wisc.edu FU National Foundation Science [DBI-1228280]; Wisconsin Cranberry Growers Association, Cranberry Institute; USDA-ARS [3655-21220-001-00] FX The authors thank Rom. 8:31-39, Eric Wiesman, and Doug Senalik for their help with different aspects of this work. This research was supported by funds from the National Foundation Science (DBI-1228280), Wisconsin Cranberry Growers Association, Cranberry Institute, and USDA-ARS (project no. 3655-21220-001-00) provided to J.E.Z. and S.A.S. NR 38 TC 12 Z9 13 U1 0 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD FEB 25 PY 2014 VL 536 IS 2 BP 336 EP 343 DI 10.1016/j.gene.2013.11.104 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA AA3VC UT WOS:000331021800016 PM 24342657 ER PT J AU Guimaraes, CT Simoes, CC Pastina, MM Maron, LG Magalhaes, JV Vasconcellos, RCC Guimaraes, LJM Lana, UGP Tinoco, CFS Noda, RW Jardim-Belicuas, SN Kochian, LV Alves, VMC Parentoni, SN AF Guimaraes, Claudia T. Simoes, Christiano C. Pastina, Maria Marta Maron, Lyza G. Magalhaes, Jurandir V. Vasconcellos, Renato C. C. Guimaraes, Lauro J. M. Lana, Ubiraci G. P. Tinoco, Carlos F. S. Noda, Roberto W. Jardim-Belicuas, Silvia N. Kochian, Leon V. Alves, Vera M. C. Parentoni, Sidney N. TI Genetic dissection of Al tolerance QTLs in the maize genome by high density SNP scan SO BMC GENOMICS LA English DT Article DE Genotyping-by-sequencing; MATE; Marker-assisted selection; Copy number variation ID QUANTITATIVE-TRAIT LOCI; ALUMINUM-ACTIVATED CITRATE; REGULATES MULTIPLE GENES; ORGANIC-ACID EXUDATION; ORYZA-SATIVA L.; ABC TRANSPORTER; MALATE TRANSPORTERS; TROPICAL MAIZE; RICE; ARABIDOPSIS AB Background: Aluminum (Al) toxicity is an important limitation to food security in tropical and subtropical regions. High Al saturation on acid soils limits root development, reducing water and nutrient uptake. In addition to naturally occurring acid soils, agricultural practices may decrease soil pH, leading to yield losses due to Al toxicity. Elucidating the genetic and molecular mechanisms underlying maize Al tolerance is expected to accelerate the development of Al-tolerant cultivars. Results: Five genomic regions were significantly associated with Al tolerance, using 54,455 SNP markers in a recombinant inbred line population derived from Cateto Al237. Candidate genes co-localized with Al tolerance QTLs were further investigated. Near-isogenic lines (NILs) developed for ZmMATE2 were as Al-sensitive as the recurrent line, indicating that this candidate gene was not responsible for the Al tolerance QTL on chromosome 5, qALT5. However, ZmNrat1, a maize homolog to OsNrat1, which encodes an Al3+ specific transporter previously implicated in rice Al tolerance, was mapped at similar to 40 Mbp from qALT5. We demonstrate for the first time that ZmNrat1 is preferentially expressed in maize root tips and is up-regulated by Al, similarly to OsNrat1 in rice, suggesting a role of this gene in maize Al tolerance. The strongest-effect QTL was mapped on chromosome 6 (qALT6), within a 0.5 Mbp region where three copies of the Al tolerance gene, ZmMATE1, were found in tandem configuration. qALT6 was shown to increase Al tolerance in maize; the qALT6-NILs carrying three copies of ZmMATE1 exhibited a two-fold increase in Al tolerance, and higher expression of ZmMATE1 compared to the Al sensitive recurrent parent. Interestingly, a new source of Al tolerance via ZmMATE1 was identified in a Brazilian elite line that showed high expression of ZmMATE1 but carries a single copy of ZmMATE1. Conclusions: High ZmMATE1 expression, controlled either by three copies of the target gene or by an unknown molecular mechanism, is responsible for Al tolerance mediated by qALT6. As Al tolerant alleles at qALT6 are rare in maize, marker-assisted introgression of this QTL is an important strategy to improve maize adaptation to acid soils worldwide. C1 [Guimaraes, Claudia T.; Pastina, Maria Marta; Magalhaes, Jurandir V.; Guimaraes, Lauro J. M.; Lana, Ubiraci G. P.; Noda, Roberto W.; Alves, Vera M. C.; Parentoni, Sidney N.] Embrapa Maize & Sorghum, Nucleus Appl Biol, BR-35701970 Sete Lagoas, MG, Brazil. [Simoes, Christiano C.; Vasconcellos, Renato C. C.; Jardim-Belicuas, Silvia N.] Univ Fed Minas Gerais, Inst Ciencias Biol, Belo Horizonte, MG, Brazil. [Maron, Lyza G.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY USA. [Tinoco, Carlos F. S.] Ctr Univ Sete Lagoas, Dept Biol, Sete Lagoas, MG, Brazil. [Kochian, Leon V.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Guimaraes, CT (reprint author), Embrapa Maize & Sorghum, Nucleus Appl Biol, Rd MG424,Km 65, BR-35701970 Sete Lagoas, MG, Brazil. EM claudia.guimaraes@embrapa.br RI Maron, Lyza/B-5644-2008; OI Maron, Lyza/0000-0001-6475-2357; Kochian, Leon/0000-0003-3416-089X FU CNPq; FAPEMIG; The McKnight Foundation CCRP; IFS; FAPEMG; CAPES; Generation Challenge Programme; Embrapa FX JVM and CTG received fellowship from CNPq, CFST was supported by FAPEMIG. We thank Beatriz Barros for technical assistance in this study. This work was supported by The McKnight Foundation CCRP, IFS, FAPEMG, CAPES, CNPq, Generation Challenge Programme and Embrapa. NR 71 TC 4 Z9 6 U1 3 U2 32 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 24 PY 2014 VL 15 AR 153 DI 10.1186/1471-2164-15-153 PG 14 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AD8OE UT WOS:000333524700002 PM 24564817 ER PT J AU Gu, T Zhu, MJ Schroyen, M Qu, L Nettleton, D Kuhar, D Lunney, JK Ross, JW Zhao, SH Tuggle, CK AF Gu, Ting Zhu, Meng-jin Schroyen, Martine Qu, Long Nettleton, Dan Kuhar, Dan Lunney, Joan K. Ross, Jason W. Zhao, Shu-hong Tuggle, Christopher K. TI Endometrial gene expression profiling in pregnant Meishan and Yorkshire pigs on day 12 of gestation SO BMC GENOMICS LA English DT Article DE Pig; Prolificacy; Endometrium; Differentially expressed genes; Implantation ID RAPID TROPHOBLASTIC ELONGATION; GROWTH-FACTOR EXPRESSION; ABORTION-PRONE MATINGS; LARGE WHITE; LITTER SIZE; OLIGONUCLEOTIDE MICROARRAY; REPRODUCTIVE-PERFORMANCE; ENDOCRINE DISRUPTION; PORCINE CONCEPTUS; BINDING-PROTEIN AB Background: Litter size in pigs is a major factor affecting the profitability in the pig industry. The peri-implantation window in pigs is characterized by the coordinated interactions between the maternal uterine endometrium and the rapidly elongating conceptuses and represents a period of time during which a large percentage of the developing conceptuses are lost. However, the gene expression and regulatory networks in the endometrium contributing to the establishment of the maternal: placental interface remain poorly understood. Results: We characterized the endometrial gene expression profile during the peri-implantation stage of development by comparing two breeds that demonstrate very different reproductive efficiencies. We employed the porcine Affymetrix GeneChip (R) to assay the transcriptomic profiles of genes expressed in the uterine endometrium obtained from Meishan and Yorkshire gilts (n = 4 for each breed) on day 12 of gestation (M12 and Y12, respectively). Total of 17,076 probesets were identified as "present" in at least two arrays. A mixed model-based statistical analysis predicted a total of 2,656 (q < 0.1) transcripts as differentially expressed between Meishan and Yorkshire pigs. Eighteen differentially expressed transcripts of interest were validated by quantitative real-time PCR. Gene ontology (GO) annotation revealed that the known functions of the differentially expressed genes were involved in a series of important biological processes relevant to early pregnancy establishment in the pig. Conclusions: The results identified endometrial gene expression profiles of two breeds differing in litter size and identified candidate genes that are related to known physiological pathways related to reproductive prolificacy. These findings provide a deeper understanding of molecular pathways differing between two breeds at the critical peri-implantation stage of pregnancy, which can be utilized to better understand the events contributing to pregnancy establishment in the pig. C1 [Gu, Ting; Zhu, Meng-jin; Zhao, Shu-hong] Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China. [Schroyen, Martine; Ross, Jason W.; Zhao, Shu-hong; Tuggle, Christopher K.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Qu, Long; Nettleton, Dan] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. [Kuhar, Dan; Lunney, Joan K.] ARS, USDA, BARC, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Zhao, SH (reprint author), Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China. EM shzhao@mail.hzau.edu.cn; cktuggle@iastate.edu FU USDA NRI [2004-35205-14202]; National Transgenic Project of China [2011ZX08006-005] FX This work was supported by USDA NRI 2004-35205-14202 and the National Transgenic Project of China (grant no. 2011ZX08006-005) NR 74 TC 10 Z9 10 U1 1 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 24 PY 2014 VL 15 AR 156 DI 10.1186/1471-2164-15-156 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AC6AQ UT WOS:000332603100002 PM 24564230 ER PT J AU Ellis, CK Stahl, RS Nol, P Waters, WR Palmer, MV Rhyan, JC VerCauteren, KC McCollum, M Salman, MD AF Ellis, Christine K. Stahl, Randal S. Nol, Pauline Waters, W. Ray Palmer, Mitchell V. Rhyan, Jack C. VerCauteren, Kurt C. McCollum, Matthew Salman, M. D. TI A Pilot Study Exploring the Use of Breath Analysis to Differentiate Healthy Cattle from Cattle Experimentally Infected with Mycobacterium bovis SO PLOS ONE LA English DT Article ID VOLATILE ORGANIC-COMPOUNDS; SOLID-PHASE MICROEXTRACTION; CHROMATOGRAPHY-MASS SPECTROMETRY; WHITE-TAILED DEER; GAS-CHROMATOGRAPHY; ELECTRONIC-NOSE; DAIRY-COWS; PULMONARY TUBERCULOSIS; DIAGNOSTIC-TECHNIQUES; EXHALED BREATH AB Bovine tuberculosis, caused by Mycobacterium bovis, is a zoonotic disease of international public health importance. Antemortem surveillance is essential for control; however, current surveillance tests are hampered by limitations affecting ease of use or quality of results. There is an emerging interest in human and veterinary medicine in diagnosing disease via identification of volatile organic compounds produced by pathogens and host-pathogen interactions. The objective of this pilot study was to explore application of existing human breath collection and analysis methodologies to cattle as a means to identify M. bovis infection through detection of unique volatile organic compounds or changes in the volatile organic compound profiles present in breath. Breath samples from 23 male Holstein calves (7 non-infected and 16 M. bovis-infected) were collected onto commercially available sorbent cartridges using a mask system at 90 days post-inoculation with M. bovis. Samples were analyzed using gas chromatography-mass spectrometry, and chromatographic data were analyzed using standard analytical chemical and metabolomic analyses, principle components analysis, and a linear discriminant algorithm. The findings provide proof of concept that breath-derived volatile organic compound analysis can be used to differentiate between healthy and M. bovis-infected cattle. C1 [Ellis, Christine K.; Salman, M. D.] Colorado State Univ, Coll Vet Med & Biomed Sci, Anim Populat Hlth Inst, Ft Collins, CO 80523 USA. [Ellis, Christine K.; Stahl, Randal S.; VerCauteren, Kurt C.] Anim Plant & Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO USA. [Nol, Pauline; Rhyan, Jack C.; McCollum, Matthew] Anim Plant & Hlth Inspect Serv, USDA, Vet Serv, Wildlife Livestock Dis Invest Team, Ft Collins, CO USA. [Waters, W. Ray; Palmer, Mitchell V.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. RP Salman, MD (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, Anim Populat Hlth Inst, Ft Collins, CO 80523 USA. EM m.d.salman@colostate.edu FU USDA-APHIS (United States Department of Agriculture's Animal and Plant Health Inspection Service) FX USDA-APHIS (United States Department of Agriculture's Animal and Plant Health Inspection Service) funded the study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 78 TC 6 Z9 6 U1 2 U2 35 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 24 PY 2014 VL 9 IS 2 AR e89280 DI 10.1371/journal.pone.0089280 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AB6EP UT WOS:000331880700027 PM 24586655 ER PT J AU Nyasembe, VO Tchouassi, DP Kirwa, HK Foster, WA Teal, PEA Borgemeister, C Torto, B AF Nyasembe, Vincent O. Tchouassi, David P. Kirwa, Hillary K. Foster, Woodbridge A. Teal, Peter E. A. Borgemeister, Christian Torto, Baldwyn TI Development and Assessment of Plant-Based Synthetic Odor Baits for Surveillance and Control of Malaria Vectors SO PLOS ONE LA English DT Article ID ANOPHELES-GAMBIAE COMPLEX; PLASMODIUM-FALCIPARUM; CARBON-DIOXIDE; WESTERN KENYA; AEDES-AEGYPTI; MOSQUITO MANAGEMENT; FIELD-EVALUATION; MATING-BEHAVIOR; DIPTERA; TRAPS AB Background: Recent malaria vector control measures have considerably reduced indoor biting mosquito populations. However, reducing the outdoor biting populations remains a challenge because of the unavailability of appropriate lures to achieve this. This study sought to test the efficacy of plant-based synthetic odor baits in trapping outdoor populations of malaria vectors. Methodology and Principal Finding: Three plant-based lures ((E)-linalool oxide [LO], (E)-linalool oxide and (E)-beta-ocimene [LO + OC], and a six-component blend comprising (E)-linalool oxide, (E)-beta-ocimene, hexanal, beta-pinene, limonene, and (E)-beta-farnesene [Blend C]), were tested alongside an animal/human-based synthetic lure (comprising heptanal, octanal, nonanal, and decanal [Blend F]) and worn socks in a malaria endemic zone in the western part of Kenya. Mosquito Magnet-X (MM-X) and lightless Centre for Disease Control (CDC) light traps were used. Odor-baited traps were compared with traps baited with either solvent alone or solvent + carbon dioxide (controls) for 18 days in a series of randomized incomplete-block designs of days x sites x treatments. The interactive effect of plant and animal/human odor was also tested by combining LO with either Blend F or worn socks. Our results show that irrespective of trap type, traps baited with synthetic plant odors compared favorably to the same traps baited with synthetic animal odors and worn socks in trapping malaria vectors, relative to the controls. Combining LO and worn socks enhanced trap captures of Anopheles species while LO + Blend F recorded reduced trap capture. Carbon dioxide enhanced total trap capture of both plant- and animal/human-derived odors. However, significantly higher proportions of male and engorged female Anopheles gambiae s.l. were caught when the odor treatments did not include carbon dioxide. Conclusion and Significance: The results highlight the potential of plant-based odors and specifically linalool oxide, with or without carbon dioxide, for surveillance and mass trapping of malaria vectors. C1 [Nyasembe, Vincent O.; Tchouassi, David P.; Kirwa, Hillary K.; Borgemeister, Christian; Torto, Baldwyn] Int Ctr Insect Physiol & Ecol, Behav & Chem Ecol Dept, Nairobi, Kenya. [Foster, Woodbridge A.] Ohio State Univ, Dept Ecol & Organismal Biol, Columbus, OH 43210 USA. [Teal, Peter E. A.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. [Borgemeister, Christian] Univ Bonn, Res Dev Ctr, Bonn, Germany. RP Torto, B (reprint author), Int Ctr Insect Physiol & Ecol, Behav & Chem Ecol Dept, POB 30772, Nairobi, Kenya. EM btorto@icipe.org FU Center for Medical, Agricultural, and Veterinary Entomology, U.S. Department of Agriculture; U.S. National Institutes of Health, National Institute of Allergy and Infectious Diseases [R01A1077722] FX This study was funded in part by the Center for Medical, Agricultural, and Veterinary Entomology, U.S. Department of Agriculture and by the U.S. National Institutes of Health, National Institute of Allergy and Infectious Diseases, grant R01A1077722 to WAF. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 83 TC 14 Z9 15 U1 0 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 24 PY 2014 VL 9 IS 2 AR e89818 DI 10.1371/journal.pone.0089818 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AB6EP UT WOS:000331880700097 PM 24587059 ER PT J AU Codling, EE Rutto, KL AF Codling, Eton E. Rutto, Kipkoriony L. TI STINGING NETTLE (URTICA DIOICA L.) GROWTH AND MINERAL UPTAKE FROM LEAD-ARSENATE CONTAMINATED ORCHARD SOILS SO JOURNAL OF PLANT NUTRITION LA English DT Article DE U; dioica; mineral nutrition; arsenic and lead contamination; food and medicinal value ID BENIGN PROSTATIC HYPERPLASIA; MEDICINAL-PLANTS; EXTRACT; SABAL; TOLERANCE; SYMPTOMS; EFFICACY; CADMIUM; MERCURY; METALS AB Growth and mineral uptake was studied in stinging nettle (Urtica dioica L.) grown on lead-arsenate contaminated orchard soils. Six soil samples including a non-orchard control were used. Uniform U. dioica seedlings were transplanted into pots arranged in a completely randomized design and raised under uniform greenhouse conditions. Shoots were harvested at day 26, and shoot (harvest 2) and root portions 50days after transplanting. All samples were oven dried, weighed, and processed for mineral analysis. Elevated arsenic levels in soil did not significantly affect nettle growth except at the highest concentration of 163mg kg(-1). There was arsenic bio-concentration in U. dioica roots and high levels of arsenic and lead in both below- and aboveground tissues. Stinging nettle is a plant of economic importance harvested for food and medicine. Our results show that humans can be exposed to toxic levels of arsenic and lead through contaminated nettle products. C1 [Codling, Eton E.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Rutto, Kipkoriony L.] Virginia State Univ, Agr Res Stn, Petersburg, VA 23806 USA. RP Rutto, KL (reprint author), Virginia State Univ, Agr Res Stn, POB 9061, Petersburg, VA 23806 USA. EM lrutto@vsu.edu NR 38 TC 1 Z9 1 U1 0 U2 31 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 0190-4167 EI 1532-4087 J9 J PLANT NUTR JI J. Plant Nutr. PD FEB 23 PY 2014 VL 37 IS 3 BP 393 EP 405 DI 10.1080/01904167.2013.859702 PG 13 WC Plant Sciences SC Plant Sciences GA 290SX UT WOS:000329779700005 ER PT J AU Kudva, IT Stanton, TB Lippolis, JD AF Kudva, Indira T. Stanton, Thaddeus B. Lippolis, John D. TI The Escherichia coli O157:H7 bovine rumen fluid proteome reflects adaptive bacterial responses SO BMC MICROBIOLOGY LA English DT Article DE O157; Rumen; LC-MS/MS; iTRAQ; Proteome; Growth-patterns ID STATISTICAL-MODEL; EPITHELIAL-CELLS; GENE-EXPRESSION; ACID TOLERANCE; FATTY-ACIDS; IN-VITRO; O157-H7; MEDIA; VIRULENCE; ADHERENCE AB Background: To obtain insights into Escherichia coli O157: H7 (O157) survival mechanisms in the bovine rumen, we defined the growth characteristics and proteome of O157 cultured in rumen fluid (RF; pH 6.0-7.2 and low volatile fatty acid content) obtained from rumen-fistulated cattle fed low protein content "maintenance diet"under diverse in vitro conditions. Results: Bottom-up proteomics (LC-MS/MS) of whole cell-lysates of O157 cultured under anaerobic conditions in filter-sterilized RF (fRF; devoid of normal ruminal microbiota) and nutrient-depleted and filtered RF (dRF) resulted in an anaerobic O157 fRF-and dRF-proteome comprising 35 proteins functionally associated with cell structure, motility, transport, metabolism and regulation, but interestingly, not with O157 virulence. Shotgun proteomics-based analysis using isobaric tags for relative and absolute quantitation used to further study differential protein expression in unfiltered RF (uRF; RF containing normal rumen microbial flora) complemented these results. Conclusions: Our results indicate that in the rumen, the first anatomical compartment encountered by this human pathogen within the cattle gastrointestinal tract (GIT), O157 initiates a program of specific gene expression that enables it to adapt to the in vivo environment, and successfully transit to its colonization sites in the bovine GIT. Further experiments in vitro using uRF from animals fed different diets and with additional O157 strains, and in vivo using rumen-fistulated cattle will provide a comprehensive understanding of the adaptive mechanisms involved, and help direct evolution of novel modalities for blocking O157 infection of cattle. C1 [Kudva, Indira T.; Stanton, Thaddeus B.] Agr Res Serv, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Lippolis, John D.] Agr Res Serv, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Kudva, IT (reprint author), Agr Res Serv, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM Indira.Kudva@ars.usda.gov NR 55 TC 1 Z9 1 U1 1 U2 15 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD FEB 21 PY 2014 VL 14 AR 48 DI 10.1186/1471-2180-14-48 PG 18 WC Microbiology SC Microbiology GA AG2SL UT WOS:000335266400003 PM 24559513 ER PT J AU Fry, DL Stephens, SL Collins, BM North, MP Franco-Vizcaino, E Gill, SJ AF Fry, Danny L. Stephens, Scott L. Collins, Brandon M. North, Malcolm P. Franco-Vizcaino, Ernesto Gill, Samantha J. TI Contrasting Spatial Patterns in Active-Fire and Fire-Suppressed Mediterranean Climate Old-Growth Mixed Conifer Forests SO PLOS ONE LA English DT Article ID PONDEROSA PINE FOREST; LAKE TAHOE BASIN; NORTH-WESTERN MEXICO; SAN-PEDRO-MARTIR; SIERRA-NEVADA; RESTORATION TREATMENTS; UNITED-STATES; JEFFREY PINE; NORTHWESTERN MEXICO; STAND STRUCTURE AB In Mediterranean environments in western North America, historic fire regimes in frequent-fire conifer forests are highly variable both temporally and spatially. This complexity influenced forest structure and spatial patterns, but some of this diversity has been lost due to anthropogenic disruption of ecosystem processes, including fire. Information from reference forest sites can help management efforts to restore forests conditions that may be more resilient to future changes in disturbance regimes and climate. In this study, we characterize tree spatial patterns using four-ha stem maps from four old-growth, Jeffrey pine-mixed conifer forests, two with active-fire regimes in northwestern Mexico and two that experienced fire exclusion in the southern Sierra Nevada. Most of the trees were in patches, averaging six to 11 trees per patch at 0.007 to 0.014 ha(-1), and occupied 27-46% of the study areas. Average canopy gap sizes (0.04 ha) covering 11-20% of the area were not significantly different among sites. The putative main effects of fire exclusion were higher densities of single trees in smaller size classes, larger proportion of trees (>= 56%) in large patches (>= 10 trees), and decreases in spatial complexity. While a homogenization of forest structure has been a typical result from fire exclusion, some similarities in patch, single tree, and gap attributes were maintained at these sites. These within-stand descriptions provide spatially relevant benchmarks from which to manage for structural heterogeneity in frequent-fire forest types. C1 [Fry, Danny L.; Stephens, Scott L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Collins, Brandon M.; North, Malcolm P.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA USA. [Collins, Brandon M.] Univ Calif Berkeley, Ctr Fire Res & Outreach, Coll Nat Resources, Berkeley, CA 94720 USA. [Franco-Vizcaino, Ernesto] Ctr Invest Cient & Educ Super Ensenada, Dept Biol Conservac, Ensenada, Baja California, Mexico. [Gill, Samantha J.] Calif Polytech State Univ San Luis Obispo, Nat Resources Management & Bioresource Dept, San Luis Obispo, CA 93407 USA. [Gill, Samantha J.] Calif Polytech State Univ San Luis Obispo, Dept Agr Engn, San Luis Obispo, CA 93407 USA. RP Fry, DL (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM dfry@berkeley.edu FU USDA Agricultural Experiment Station Funds from University of California, Berkeley FX This project was supported by the USDA Agricultural Experiment Station Funds from University of California, Berkeley. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 108 TC 15 Z9 15 U1 3 U2 39 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 20 PY 2014 VL 9 IS 2 AR e88985 DI 10.1371/journal.pone.0088985 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AB3UC UT WOS:000331714700047 PM 24586472 ER PT J AU Deng, DF Ju, ZY Dominy, WG Conquest, L Smiley, S Bechtel, PJ AF Deng, D. F. Ju, Z. Y. Dominy, W. G. Conquest, L. Smiley, S. Bechtel, P. J. TI Effect of replacing dietary menhaden oil with pollock or soybean oil on muscle fatty acid composition and growth performance of juvenile Pacific threadfin (Polydactylus sexfilis) SO AQUACULTURE LA English DT Article DE Fatty acid; Fish oil; Growth performance; Moi; Vegetable oil ID SEABREAM SPARUS-AURATA; TROUT ONCORHYNCHUS-MYKISS; FISH-OIL; VEGETABLE-OILS; SCIAENOPS-OCELLATUS; TOTAL REPLACEMENT; LINOLENIC ACID; LINSEED OIL; RED DRUM; REQUIREMENT AB This study investigated effect of different dietary lipid sources on juvenile Pacific threadfin (Polydactylus sexfilis), a tropical marine fish, based on growth performance, proximate composition of whole fish and muscle fatty acid composition. The objectives were 1) to compare the nutritional value of menhaden oil to pollock oil, a product generated by the Alaskan fish processing industry; and 2) to evaluate the potential of replacing pollock oil with soybean oil in diets for juvenile Pacific threadfin. Six experimental diets were formulated to contain 42% crude protein and 12% crude lipid including 4% lipid from fishmeal and 8% added oil. The 8% added oil was provided by menhaden oil pollock oil (P) or soybean (S) oil mixed with pollock oil. Diets containing the mixed oil were named as P8S0, P6S2, P4S4, P2S6, and P0S8 to denote the level of pollock oil (decreasing from 8 to 0%) and soybean oil (increasing from 0 to 8%). Pacific threadfin (average body weight of 18.6 g) were fed each of the experimental diets for 8 weeks. Growth performance, proximate composition of whole body and muscle were similar among fish fed with different diets. Pollock oil and menhaden oil were shown to have a similar effect on the nutritional composition of fish or tissue. In contrast, the diet containing 8% soybean oil significantly increased liver lipid content compared to the other test diets. The fatty acid composition of muscle reflected the dietary fatty acids. Fish fed the soybean oil diets exhibited significantly lower levels of n-3 long chain polyunsaturated fatty acid (n-3 LC-PUFA) and higher levels of 18: 2n-6 and 18:3n-3 than those fed the fish oil diets. Results of this study suggest that pollock oil can replace menhaden oil without any adverse effect on growth performance or nutritional quality of fish. Total replacement of pollock oil with soybean oil had no detrimental effect on growth performance of juvenile Pacific threadfin but altered the fatty acid composition of fish muscle tissue. These results are useful in formulating practical feeds at reduced cost for Pacific threadfin culture. However, a longer term study will be needed to investigate whether the changes in muscle fatty acid composition will influence the welfare as well as the market value of Pacific threadfin. (C) 2014 Elsevier B. V. All rights reserved. C1 [Deng, D. F.; Ju, Z. Y.; Dominy, W. G.; Conquest, L.] Ocean Inst, Waimanalo, HI 96795 USA. [Smiley, S.] Univ Alaska Fairbanks, Fishery Ind Technol Ctr, Kodiak, AK 99615 USA. [Bechtel, P. J.] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. RP Deng, DF (reprint author), Ocean Inst, 41-202 Kalanianaole Hwy, Waimanalo, HI 96795 USA. EM dfdeng@oceanicinstitute.org FU U.S. Department of Agriculture, Agricultural Research Service [58-5341-9-441]; University of Alaska, Fairbanks [UAF-10-0043] FX This research is funded by the U.S. Department of Agriculture, Agricultural Research Service (No 58-5341-9-441) through a Specific Cooperative Agreement with the University of Alaska, Fairbanks UAF-10-0043. We acknowledge the staff members of the Aquatic Feeds and Nutrition Department, Oceanic Institute for their assistance in managing the feeding trial, data collection and manuscript preparation. We would also like to thank Dr. Robert P. Wilson for his comments and assistance in editing this manuscript. NR 46 TC 4 Z9 5 U1 1 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD FEB 20 PY 2014 VL 422 BP 91 EP 97 DI 10.1016/j.aquaculture.2013.11.032 PG 7 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 296YR UT WOS:000330221900012 ER PT J AU Newell, MA Vogel, KE Adams, M Aydin, N Bodnar, AL Ali, M Lauter, ANM Scott, MP AF Newell, Mark A. Vogel, Karla E. Adams, Marie Aydin, Nevzat Bodnar, Anastasia L. Ali, Muhammad Lauter, Adrienne N. Moran Scott, M. Paul TI Genetic and biochemical differences in populations bred for extremes in maize grain methionine concentration SO BMC PLANT BIOLOGY LA English DT Article DE Methionine; Breeding; Association mapping; Sulfur assimilation; Storage proteins ID RICH STORAGE PROTEIN; SELECTION; ACCUMULATION; EXPRESSION; GERMPLASM; PATHWAYS; KERNELS; HYBRIDS AB Background: Methionine is an important nutrient in animal feed and several approaches have been developed to increase methionine concentration in maize (Zea mays L.) grain. One approach is through traditional breeding using recurrent selection. Using divergent selection, genetically related populations with extreme differences in grain methionine content were produced. In order to better understand the molecular mechanisms controlling grain methionine content, we examined seed proteins, transcript levels of candidate genes, and genotypes of these populations. Results: Two populations were selected for high or low methionine concentration for eight generations and 40 and 56% differences between the high and low populations in grain methionine concentration were observed. Mean values between the high and low methionine populations differed by greater than 1.5 standard deviations in some cycles of selection. Other amino acids and total protein concentration exhibited much smaller changes. In an effort to understand the molecular mechanisms that contribute to these differences, we compared transcript levels of candidate genes encoding high methionine seed storage proteins involved in sulfur assimilation or methionine biosynthesis. In combination, we also explored the genetic mechanisms at the SNP level through implementation of an association analysis. Significant differences in methionine-rich seed storage protein genes were observed in comparisons of high and low methionine populations, while transcripts of seed storage proteins lacking high levels of methionine were unchanged. Seed storage protein levels were consistent with transcript levels. Two genes involved in sulfur assimilation, Cys2 and CgS1 showed substantial differences in allele frequencies when two selected populations were compared to the starting populations. Major genes identified across cycles of selection by a high-stringency association analysis included dzs18, wx, dzs10, and zp27. Conclusions: We hypothesize that transcriptional changes alter sink strength by altering the levels of methionine-rich seed storage proteins. To meet the altered need for sulfur, a cysteine-rich seed storage protein is altered while sulfur assimilation and methionine biosynthesis throughput is changed by selection for certain alleles of Cys2 and CgS1. C1 [Newell, Mark A.] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA. [Vogel, Karla E.; Adams, Marie; Bodnar, Anastasia L.] Iowa State Univ, Interdept Genet Grad Program, Ames, IA 50011 USA. [Vogel, Karla E.] Monsanto Co, St Louis, MO 63137 USA. [Aydin, Nevzat] Karamanoglu Mehmetbey Univ, Fac Engn, Dept Bioengn, TR-70100 Karaman, Turkey. [Ali, Muhammad] North West Frontier Prov Agr Univ, Peshawar, Pakistan. [Lauter, Adrienne N. Moran; Scott, M. Paul] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Scott, MP (reprint author), USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. EM paul.scott@ars.usda.gov RI Aydin, Nevzat /F-6743-2011 FU USDA-NIFA-OREI; Higher Education Ministry of Pakistan FX The authors wish to thank the Monsanto genotyping laboratory in Ankeny, Iowa for their assistance with genotyping and hourly workers and technicians who assisted with the breeding program. Names are necessary to report factually on the available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by the USDA implies no approval of the product to the exclusion of others that may be suitable. This work was funded in part by grants from USDA-NIFA-OREI and the Higher Education Ministry of Pakistan. NR 32 TC 3 Z9 3 U1 0 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD FEB 19 PY 2014 VL 14 AR 49 DI 10.1186/1471-2229-14-49 PG 13 WC Plant Sciences SC Plant Sciences GA AF9UV UT WOS:000335061100001 PM 24552611 ER PT J AU Fu, YW Zhang, QZ Xu, DH Xia, H Cai, XX Wang, B Liang, JH AF Fu, YaoWu Zhang, QiZhong Xu, De-Hai Xia, Huan Cai, XinXing Wang, Bin Liang, Jinghan TI Parasiticidal effects of Morus alba root bark extracts against Ichthyophthirius multifiliis infecting grass carp SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE Antiparasitic effect; White mulberry; Ich; Lethal dose; Acetone; Ethyl acetate ID GOLDFISH CARASSIUS-AURATUS; CHANNEL CATFISH; POTASSIUM-PERMANGANATE; COPPER-SULFATE; ACUTE TOXICITY; THERONTS; EFFICACY; FOUQUET; WATER; FISH AB Ichthyophthirius multifiliis (Ich), an important fish parasite, can cause significant losses in aquaculture. To find efficacious drugs to control Ich, the root bark of white mulberry Morus alba was evaluated for its antiprotozoal activity. Bark was powdered and extracted with 1 of 5 organic solvents: petroleum ether, chloroform, ethyl acetate, acetone, or methanol. The extracts were concentrated, dissolved in 0.1% (v/v) DMSO, and used for anti-Ich trials. Acetone and ethyl acetate extracts significantly reduced the survival of Ich tomonts and theronts. In vitro, acetone extract at 25 mg l(-1) killed all non- encysted tomonts, at 50 mg l(-1) eradicated all encysted tomonts, and at 8 mg l(-1) caused mortality of all theronts. Ethyl acetate extract at 50 mg l(-1) eliminated all non- encysted tomonts, at 100 mg l(-1) killed all encysted tomonts and terminated tomont reproduction, and at 8 mg l(-1) killed all theronts. Low concentrations (2 and 4 mg l(-1)) of acetone and ethyl acetate extracts could not kill all theronts after 4 h exposure, but a significant decrease in theront infectivity was observed following 30 min of pretreatment with the extracts. The 96 h LC50 values of acetone and ethyl acetate extracts to grass carp were 79.46 and 361.05 mg l(-1), i.e. much higher than effective doses for killing Ich theronts (8 mg l(-1) for both extracts) and non-encysted tomonts (12.5 and 25 mg l(-1), respectively). Thus M. alba extract may be a potential new, safe, and efficacious drug to control Ich. C1 [Fu, YaoWu; Zhang, QiZhong; Xia, Huan; Cai, XinXing; Wang, Bin; Liang, Jinghan] Jinan Univ, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn, Inst Hydrobiol,Minist Educ,Guangdong Higher Educ, Key Lab Aquat Eutrophicat & Control Harmful Algal, Guangzhou 510632, Guangdong, Peoples R China. [Xu, De-Hai] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP Zhang, QZ (reprint author), Jinan Univ, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn, Inst Hydrobiol,Minist Educ,Guangdong Higher Educ, Key Lab Aquat Eutrophicat & Control Harmful Algal, Guangzhou 510632, Guangdong, Peoples R China. EM zhangqzdr@126.com FU National High Technology Research and Development Program of China (863 Program) [2011AA10A216]; Guangzhou Science and Technology Project [2013 J4100047]; Fundamental Research Funds for the Central Universities [21612111, 21613105] FX This work was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2011AA10A216) and Guangzhou Science and Technology Project (No. 2013 J4100047) as well as the Fundamental Research Funds for the Central Universities (21612111, 21613105). NR 41 TC 8 Z9 8 U1 2 U2 10 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD FEB 19 PY 2014 VL 108 IS 2 BP 129 EP 136 DI 10.3354/dao02708 PG 8 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA AE5PS UT WOS:000334041700004 PM 24553418 ER PT J AU Radley-Crabb, HG Marini, JC Sosa, HA Castillo, LI Grounds, MD Fiorotto, ML AF Radley-Crabb, Hannah G. Marini, Juan C. Sosa, Horacio A. Castillo, Liliana I. Grounds, Miranda D. Fiorotto, Marta L. TI Dystropathology Increases Energy Expenditure and Protein Turnover in the Mdx Mouse Model of Duchenne Muscular Dystrophy SO PLOS ONE LA English DT Article ID SKELETAL-MUSCLE REGENERATION; GENE-EXPRESSION; MYOGENIC CELLS; METABOLIC-RATE; BODY-COMPOSITION; IN-VIVO; MICE; GROWTH; DIAPHRAGM; ACID AB The skeletal muscles in Duchenne muscular dystrophy and the mdx mouse model lack functional dystrophin and undergo repeated bouts of necrosis, regeneration, and growth. These processes have a high metabolic cost. However, the consequences for whole body energy and protein metabolism, and on the dietary requirements for these macronutrients at different stages of the disease, are not well-understood. This study used juvenile (4- to 5- wk-old) and adult (12- to 14- wk-old) male dystrophic C57BL/10ScSn-mdx/J and age-matched C57BL/10ScSn/J control male mice to measure total and resting energy expenditure, food intake, spontaneous activity, body composition, whole body protein turnover, and muscle protein synthesis rates. In juvenile mdx mice that have extensive muscle damage, energy expenditure, muscle protein synthesis, and whole body protein turnover rates were higher than in age-matched controls. Adaptations in food intake and decreased activity were insufficient to meet the increased energy and protein needs of juvenile mdx mice and resulted in stunted growth. In (non-growing) adult mdx mice with less severe dystropathology, energy expenditure, muscle protein synthesis, and whole body protein turnover rates were also higher than in age-matched controls. Food intake was sufficient to meet their protein and energy needs, but insufficient to result in fat deposition. These data show that dystropathology impacts the protein and energy needs of mdx mice and that tailored dietary interventions are necessary to redress this imbalance. If not met, the resultant imbalance blunts growth, and may limit the benefits of therapies designed to protect and repair dystrophic muscles. C1 [Radley-Crabb, Hannah G.] Curtin Univ, CHIRI Biosci Res Precinct, Sch Biomed Sci, Perth, WA 6845, Australia. [Radley-Crabb, Hannah G.; Grounds, Miranda D.] Univ Western Australia, Sch Anat Physiol & Human Biol, Perth, WA 6009, Australia. [Marini, Juan C.; Sosa, Horacio A.; Castillo, Liliana I.; Fiorotto, Marta L.] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77041 USA. [Marini, Juan C.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. RP Fiorotto, ML (reprint author), ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77041 USA. EM martaf@bcm.edu FU Australian Postgraduate Award; UWA; USDA/ARS FX H. Radley-Crabb was supported by an Australian Postgraduate Award and a Grant for Research Student Training provided by UWA. Support for isotope analyses was provided by USDA/ARS funds. Body composition, energy balance, and food intake measurements were performed in the Mouse Metabolic Research Unit at the USDA/ARS Children's Nutrition Research Center, which is supported by funds from the USDA/ARS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 81 TC 10 Z9 10 U1 0 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 19 PY 2014 VL 9 IS 2 AR e89277 DI 10.1371/journal.pone.0089277 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AB3TA UT WOS:000331711900121 PM 24586653 ER PT J AU Unbehend, M Hanniger, S Vasquez, GM Juarez, ML Reisig, D McNeil, JN Meagher, RL Jenkins, DA Heckel, DG Groot, AT AF Unbehend, Melanie Haenniger, Sabine Vasquez, Gissella M. Laura Juarez, Maria Reisig, Dominic McNeil, Jeremy N. Meagher, Robert L. Jenkins, David A. Heckel, David G. Groot, Astrid T. TI Geographic Variation in Sexual Attraction of Spodoptera frugiperda Corn- and Rice-Strain Males to Pheromone Lures SO PLOS ONE LA English DT Article ID FALL ARMYWORM LEPIDOPTERA; CUTWORM MOTH LEPIDOPTERA; BORER OSTRINIA-NUBILALIS; NOCTUIDAE HOST STRAINS; GENETIC-STRUCTURE; PHYSALAEMUS-PUSTULOSUS; ENVIRONMENTAL-FACTORS; ADVERTISEMENT CALL; RELATIVE-HUMIDITY; FIELD-EVALUATION AB The corn-and rice-strains of Spodoptera frugiperda exhibit several genetic and behavioral differences and appear to be undergoing ecological speciation in sympatry. Previous studies reported conflicting results when investigating male attraction to pheromone lures in different regions, but this could have been due to inter-strain and/or geographic differences. Therefore, we investigated whether corn- and rice-strain males differed in their response to different synthetic pheromone blends in different regions in North America, the Caribbean and South America. All trapped males were strain-typed by two strain-specific mitochondrial DNA markers. In the first experiment, we found a nearly similar response of corn- and rice-strain males to two different 4-component blends, resembling the corn-and rice-strain female blend we previously described from females in Florida. This response showed some geographic variation in fields in Canada, North Carolina, Florida, Puerto Rico, and South America (Peru, Argentina). In dose-response experiments with the critical secondary sex pheromone component (Z)-7-dodecenyl acetate (Z7-12:OAc), we found some strain-specific differences in male attraction. While the response to Z7-12: OAc varied geographically in the corn-strain, rice-strain males showed almost no variation. We also found that the minor compound (Z)-11-hexadecenyl acetate (Z11-16:OAc) did not increase attraction of both strains in Florida and of corn-strain males in Peru. In a fourth experiment, where we added the stereo-isomer of the critical sex pheromone component, (E)-7-dodecenyl acetate, to the major pheromone component (Z)-9-tetradecenyl acetate (Z9-14:OAc), we found that this compound was attractive to males in North Carolina, but not to males in Peru. Overall, our results suggest that both strains show rather geographic than strain-specific differences in their response to pheromone lures, and that regional sexual communication differences might cause geographic differentiation between populations. C1 [Unbehend, Melanie; Haenniger, Sabine; Heckel, David G.; Groot, Astrid T.] MPICE, Dept Entomol, Jena, Germany. [Vasquez, Gissella M.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Laura Juarez, Maria] Secc Zool Agr, Estn Expt Agroind Obispo Colombres, San Miguel De Tucuman, Argentina. [Reisig, Dominic] N Carolina State Univ, Dept Entomol, Plymouth, NC USA. [McNeil, Jeremy N.] Univ Western Ontario, Dept Biol, London, ON, Canada. [Meagher, Robert L.] USDA, Insect Behav & Biocontrol Res Unit, Gainesville, FL USA. [Jenkins, David A.] USDA, Mayaguez, PR USA. [Groot, Astrid T.] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands. RP Unbehend, M (reprint author), MPICE, Dept Entomol, Jena, Germany. EM munbehend@ice.mpg.de RI Groot, Astrid/A-8954-2013; Heckel, David/K-2138-2013 OI Groot, Astrid/0000-0001-9595-0161; Heckel, David/0000-0001-8991-2150 FU German Science Foundation [GR3627/2-1]; National Science Foundation [IOS-1052238, NSF-DEB-1025217]; Max Planck Gesellschaft FX This research was partly funded by the German Science Foundation (www.dfg.de; grant number GR3627/2-1), the National Science Foundation (www.nsf.gov; award IOS-1052238 and NSF-DEB-1025217), and the Max Planck Gesellschaft (www.mpg.de). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 84 TC 8 Z9 8 U1 7 U2 41 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 19 PY 2014 VL 9 IS 2 AR e89255 DI 10.1371/journal.pone.0089255 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AB3TA UT WOS:000331711900115 PM 24586634 ER PT J AU Akond, M Liu, SM Kantartzi, SK Meksem, K Bellaloui, N Lightfoot, DA Yuan, JZ Wang, DC Kassem, MA AF Akond, Masum Liu, Shiming Kantartzi, Stella K. Meksem, Khalid Bellaloui, Nacer Lightfoot, David A. Yuan, Jiazheng Wang, Dechun Kassem, My Abdelmajid TI Quantitative Trait Loci for Seed Isoflavone Contents in 'MD96-5722' by 'Spencer' Recombinant Inbred Lines of Soybean SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE soybean; SNP linkage map; QTL; RIL; daidzein; genistein; glycitein; isoflavones; 'MD96-5722'; 'Spencer' ID GENETIC-LINKAGE MAP; PHYTOESTROGENS; ENVIRONMENT; MECHANISM; GENOTYPE; CELLS AB Isoflavones from soybeans [Glycine max (L.) Mem] have a significant impact on human health to reduce the risk of several major diseases. Breeding soybean for high isoflavone content in the seed is possible through marker-assisted selection (MAS) which can be based on quantitative trait loci (QTL). The objective of this study was to identify QTL controlling isoflavone content in a set of 'MD96-5722' by 'Spencer' recombinant inbred line (RIL) populations of soybean. Wide variations were found for seed concentrations of daidzein, glycitein, genistein, and total isoflavones among the RIL populations. Three QTL were identified on three different linkage groups (LG) represented by three different chromosomes (Chr). One QTL that controlled daidzein content was identified on LG A1 (Chr 5), and two QTL that underlay glycitein content were identified on LG K (Chr 9) and LG B2 (Chr 14). Identified QTL could be functional in developing soybean with preferable isoflavone concentrations in the seeds through MAS. C1 [Akond, Masum; Kassem, My Abdelmajid] Fayetteville State Univ, Dept Biol Sci, Plant Genom & Biotechnol Lab, Fayetteville, NC 28301 USA. [Liu, Shiming; Kantartzi, Stella K.; Meksem, Khalid; Lightfoot, David A.] So Illinois Univ, Dept Plant Soil & Agr Syst, Carbondale, IL 62901 USA. [Bellaloui, Nacer] ARS, Crop Genet Res Unit, USDA, Stoneville, MS 38776 USA. [Yuan, Jiazheng; Wang, Dechun] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Kassem, MA (reprint author), Fayetteville State Univ, Dept Biol Sci, Plant Genom & Biotechnol Lab, Fayetteville, NC 28301 USA. EM mkassem@uncfsu.edu OI Lightfoot, David/0000-0002-5725-4381 FU Department of Defense (DOD) [W911NF-11-1-0178]; U.S. Department of Agriculture, Agricultural Research Service [6402-21220-012-00D] FX This work was supported by the Department of Defense (DOD) through Grant W911NF-11-1-0178 to M.A.K and S.K. This research was partially funded by the U.S. Department of Agriculture, Agricultural Research Service Project 6402-21220-012-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 41 TC 6 Z9 7 U1 3 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 19 PY 2014 VL 62 IS 7 BP 1464 EP 1468 DI 10.1021/jf4040173 PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AB4SE UT WOS:000331779400004 PM 24499298 ER PT J AU Liu, YT Blumberg, JB Chen, CYO AF Liu, Yuntao Blumberg, Jeffrey B. Chen, C. -Y. Oliver TI Quantification and Bioaccessibility of California Pistachio Bioactives SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE extraction; quantification; bioaccessibility; pistachios; tocols; polyphenols; carotenoids ID PERFORMANCE LIQUID-CHROMATOGRAPHY; VERA L.; BETA-CAROTENE; UNITED-STATES; ALMOND SKINS; ANTIOXIDANT; TOCOPHEROLS; POLYPHENOLS; NUTS; ANTHOCYANINS AB The content of carotenoids, chlorophylls, phenolics, and tocols in pistachios (Pistacia vera L.) has not been methodically quantified. The objective of this study was to first optimize extraction protocols for lipophilic nutrients and then quantify the content of two phenolic acids, nine flavonoids, four carotenoids, two chlorophylls, and three tocols in the skin, nutmeat, and whole nut of California pistachios. The dominant bioactives in whole pistachios are lutein [42.35 mu g/g fresh weight (FW)], chlorophyll a (142.24 mu g/g FW), gamma-tocopherol (182.20 mu g/g FW), flavan-3-ols (catechins) (199.18 mu g/g FW), luteolin (217.89 mu g/g FW), myricetin (135.18 mu g/g FW), and cyanidin-3-galactose (38.34 mu g/g FW) in each nutrient class. Most phenolics are present in the skin, while the lipophilic nutrients are dominantly present in the nutmeat. Digestion with a gastrointestinal mimic showed <10% of most hydrophilic compounds are released from pistachio matrices. In conclusion, 9 lipophilic and 11 hydrophilic bioactives in pistachios are systematically quantified. C1 [Liu, Yuntao; Blumberg, Jeffrey B.; Chen, C. -Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, Boston, MA 02111 USA. [Liu, Yuntao] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China. RP Chen, CYO (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, 711 Washington St, Boston, MA 02111 USA. EM Oliver.Chen@tufts.edu OI Liu, Yuntao/0000-0002-8320-8725 FU U.S. Department of Agriculture (USDA)/Agricultural Research Service [58-1950-0-014]; American Pistachio Growers FX This work was supported by the American Pistachio Growers and the U.S. Department of Agriculture (USDA)/Agricultural Research Service (Cooperative Agreement No. 58-1950-0-014). NR 37 TC 9 Z9 9 U1 5 U2 30 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 19 PY 2014 VL 62 IS 7 BP 1550 EP 1556 DI 10.1021/jf4046864 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AB4SE UT WOS:000331779400016 PM 24460079 ER PT J AU Lumpkin, C Fellman, JK Rudell, DR Mattheis, J AF Lumpkin, Christie Fellman, John K. Rudell, David R. Mattheis, James TI 'Scarlett Spur Red Delicious' Apple Volatile Production Accompanying Physiological Disorder Development during Low pO(2) Controlled Atmosphere Storage SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE anaerobic metabolism; low pO(2) stress; alcohols; esters; fruit quality; physiological disorders ID LOW-OXYGEN STRESS; ULTRA-LOW OXYGEN; SUPERFICIAL SCALD; FUJI APPLES; AROMA; FRUIT; BIOSYNTHESIS; METABOLISM; DIPHENYLAMINE; ALCOHOLS AB Apple (Malus domestica Borkh.) fruit volatile production is regulated by a variety of factors including low oxygen storage conditions. This study examined the impact of low pO(2) controlled atmospheres on 'Scarlett Spur Red Delicious' apple volatile production and disorder development. Accumulation of apple volatile compounds was characterized during long-term cold storage at 0.5 degrees C in air or low pO(2) (0.3, 0.8, or 1.5 kPa) with 1 kPa CO2. Volatile accumulation differed quantitatively with pO(2) as acetaldehyde, ethanol, and ethyl ester accumulation increased with decreased pO(2) during the first weeks in storage. Differences in volatile accumulation among atmospheres were evident through 6 months. The rate of ethanol accumulation increased with decreased pO(2) and could potentially be used to monitor low O-2 stress. Incidence of low oxygen disorders after 9 months was highest in fruit held at the lowest pO(2). The sesquiterpene alpha-farnesene was not detected throughout the storage period. C1 [Lumpkin, Christie; Fellman, John K.] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Rudell, David R.; Mattheis, James] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Mattheis, J (reprint author), USDA ARS, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM James.Mattheis@ars.usda.gov FU Washington Tree Fruit Research Commission FX Supplemental funding for this research was received from the Washington Tree Fruit Research Commission. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 42 TC 7 Z9 7 U1 3 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 19 PY 2014 VL 62 IS 7 BP 1741 EP 1754 DI 10.1021/jf405267b PG 14 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AB4SE UT WOS:000331779400040 PM 24502565 ER PT J AU Gladine, C Newman, JW Durand, T Pedersen, TL Galano, JM Demougeot, C Berdeaux, O Pujos-Guillot, E Mazur, A Comte, B AF Gladine, Cecile Newman, John W. Durand, Thierry Pedersen, Theresa L. Galano, Jean-Marie Demougeot, Celine Berdeaux, Olivier Pujos-Guillot, Estelle Mazur, Andrzej Comte, Blandine TI Lipid Profiling following Intake of the Omega 3 Fatty Acid DHA Identifies the Peroxidized Metabolites F-4-Neuroprostanes as the Best Predictors of Atherosclerosis Prevention SO PLOS ONE LA English DT Article ID POLYUNSATURATED FATTY-ACIDS; DOCOSAHEXAENOIC ACID; EICOSAPENTAENOIC ACID; IN-VIVO; DEFICIENT MICE; FISH INTAKE; RATS; TRANSFORMATIONS; NEUROPROSTANES; QUANTIFICATION AB The anti-atherogenic effects of omega 3 fatty acids, namely eicosapentaenoic (EPA) and docosahexaenoic acids (DHA) are well recognized but the impact of dietary intake on bioactive lipid mediator profiles remains unclear. Such a profiling effort may offer novel targets for future studies into the mechanism of action of omega 3 fatty acids. The present study aimed to determine the impact of DHA supplementation on the profiles of polyunsaturated fatty acids (PUFA) oxygenated metabolites and to investigate their contribution to atherosclerosis prevention. A special emphasis was given to the nonenzymatic metabolites knowing the high susceptibility of DHA to free radical-mediated peroxidation and the increased oxidative stress associated with plaque formation. Atherosclerosis prone mice (LDLR-/-) received increasing doses of DHA (0, 0.1, 1 or 2% of energy) during 20 weeks leading to a dose-dependent reduction of atherosclerosis (R-2 = 0.97, p = 0.02), triglyceridemia (R-2 = 0.97, p = 0.01) and cholesterolemia (R-2 = 0.96, p, 0.01). Targeted lipidomic analyses revealed that both the profiles of EPA and DHA and their corresponding oxygenated metabolites were substantially modulated in plasma and liver. Notably, the hepatic level of F-4-neuroprostanes, a specific class of DHA peroxidized metabolites, was strongly correlated with the hepatic DHA level. Moreover, unbiased statistical analysis including correlation analyses, hierarchical cluster and projection to latent structure discriminate analysis revealed that the hepatic level of F4-neuroprostanes was the variable most negatively correlated with the plaque extent (p<0.001) and along with plasma EPA-derived diols was an important mathematical positive predictor of atherosclerosis prevention. Thus, oxygenated n-3 PUFAs, and F-4-neuroprostanes in particular, are potential biomarkers of DHA-associated atherosclerosis prevention. While these may contribute to the anti-atherogenic effects of DHA, further in vitro investigations are needed to confirm such a contention and to decipher the molecular mechanisms of action. C1 [Gladine, Cecile; Pujos-Guillot, Estelle; Mazur, Andrzej; Comte, Blandine] Univ Auvergne, Clermont Univ, CRNH Auvergne, UMR1019,INRA,UNH, Clermont Ferrand, France. [Newman, John W.; Pedersen, Theresa L.] ARS, Obes & Metab Res Unit, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Durand, Thierry; Galano, Jean-Marie] Univ Montpellier I, IBMM, CNRS, UMR 5247, Montpellier, France. [Durand, Thierry; Galano, Jean-Marie] Univ Montpellier 2, IBMM, CNRS, UMR 5247, Montpellier, France. [Demougeot, Celine] Univ Franche Comte, EA Fonct & Dysfonct Epitheliales 4267, F-25030 Besancon, France. [Berdeaux, Olivier] CNRS, Ctr Sci Gout & Alimentat, UMR6265, Dijon, France. [Berdeaux, Olivier] INRA, Ctr Sci Gout & Alimentat, UMR1324, F-21034 Dijon, France. [Berdeaux, Olivier] Univ Bourgogne, Ctr Sci Gout & Alimentat, UMR, Dijon, France. [Pujos-Guillot, Estelle] INRA, Plateforme Explorat Metab, UMR 1019, Clermont Ferrand, France. RP Comte, B (reprint author), Univ Auvergne, Clermont Univ, CRNH Auvergne, UMR1019,INRA,UNH, Clermont Ferrand, France. EM blandine.comte@clermont.inra.fr FU intramural French National Institute for Agricultural Research (INRA); United States Department of Agriculture-Agricultural Research Service (USDA-ARS) [5306-51530-019-00D] FX The project was partially supported by intramural French National Institute for Agricultural Research (INRA, http://www.alimh.inra.fr/) and United States Department of Agriculture-Agricultural Research Service (USDA-ARS, http://www.ars.usda.gov/main/main.htm), Project 5306-51530-019-00D (JWN). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 52 TC 29 Z9 29 U1 2 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 18 PY 2014 VL 9 IS 2 AR e89393 DI 10.1371/journal.pone.0089393 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AB3RA UT WOS:000331706700142 PM 24558496 ER PT J AU Lin, L Tian, SL Kaeppler, S Liu, ZR An, YQ AF Lin, Li Tian, Shulan Kaeppler, Shawn Liu, Zongrang An, Yong-Qiang (Charles) TI Conserved Transcriptional Regulatory Programs Underlying Rice and Barley Germination SO PLOS ONE LA English DT Article ID SEED-GERMINATION; ARABIDOPSIS-THALIANA; ACTIN SUBCLASS; ALPHA-AMYLASE; ABSCISIC-ACID; BETA-AMYLASE; EXPRESSION; GENES; GIBBERELLIN; ENDOSPERM AB Germination is a biological process important to plant development and agricultural production. Barley and rice diverged 50 million years ago, but share a similar germination process. To gain insight into the conservation of their underlying gene regulatory programs, we compared transcriptomes of barley and rice at start, middle and end points of germination, and revealed that germination regulated barley and rice genes (BRs) diverged significantly in expression patterns and/or protein sequences. However, BRs with higher protein sequence similarity tended to have more conserved expression patterns. We identified and characterized 316 sets of conserved barley and rice genes (cBRs) with high similarity in both protein sequences and expression patterns, and provided a comprehensive depiction of the transcriptional regulatory program conserved in barley and rice germination at gene, pathway and systems levels. The cBRs encoded proteins involved in a variety of biological pathways and had a wide range of expression patterns. The cBRs encoding key regulatory components in signaling pathways often had diverse expression patterns. Early germination up-regulation of cell wall metabolic pathway and peroxidases, and late germination up-regulation of chromatin structure and remodeling pathways were conserved in both barley and rice. Protein sequence and expression pattern of a gene change quickly if it is not subjected to a functional constraint. Preserving germination-regulated expression patterns and protein sequences of those cBRs for 50 million years strongly suggests that the cBRs are functionally significant and equivalent in germination, and contribute to the ancient characteristics of germination preserved in barley and rice. The functional significance and equivalence of the cBR genes predicted here can serve as a foundation to further characterize their biological functions and facilitate bridging rice and barley germination research with greater confidence. C1 [Lin, Li; Tian, Shulan; An, Yong-Qiang (Charles)] ARS, USDA, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO USA. [Tian, Shulan] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Lin, Li; Kaeppler, Shawn] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Liu, Zongrang] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. RP An, YQ (reprint author), ARS, USDA, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO USA. EM yong-qiang.an@ars.usda.gov OI Kaeppler, Shawn/0000-0002-5964-1668 FU USDA-ARS; American Malting and Barley Association, Inc. FX The research is supported by funds from USDA-ARS and American Malting and Barley Association, Inc. to Yong-Qiang An. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 49 TC 1 Z9 1 U1 0 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 18 PY 2014 VL 9 IS 2 AR e87261 DI 10.1371/journal.pone.0087261 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AB3RA UT WOS:000331706700012 PM 24558366 ER PT J AU Liu, SX Gao, GT Palti, Y Cleveland, BM Weber, GM Rexroad, CE AF Liu, Sixin Gao, Guangtu Palti, Yniv Cleveland, Beth M. Weber, Gregory M. Rexroad, Caird E., III TI RNA-seq Analysis of Early Hepatic Response to Handling and Confinement Stress in Rainbow Trout SO PLOS ONE LA English DT Article ID GROWTH-FACTOR-I; IGF-BINDING PROTEINS; ONCORHYNCHUS-MYKISS; GENE-EXPRESSION; GLUCOCORTICOID-RECEPTOR; DISEASE RESISTANCE; CORTISOL RESPONSE; PRE-SLAUGHTER; HEAT-STRESS; TRANSCRIPTOME AB Fish under intensive rearing conditions experience various stressors which have negative impacts on survival, growth, reproduction and fillet quality. Identifying and characterizing the molecular mechanisms underlying stress responses will facilitate the development of strategies that aim to improve animal welfare and aquaculture production efficiency. In this study, we used RNA-seq to identify transcripts which are differentially expressed in the rainbow trout liver in response to handling and confinement stress. These stressors were selected due to their relevance in aquaculture production. Total RNA was extracted from the livers of individual fish in five tanks having eight fish each, including three tanks of fish subjected to a 3 hour handling and confinement stress and two control tanks. Equal amount of total RNA of six individual fish was pooled by tank to create five RNA-seq libraries which were sequenced in one lane of Illumina HiSeq 2000. Three sequencing runs were conducted to obtain a total of 491,570,566 reads which were mapped onto the previously generated stress reference transcriptome to identify 316 differentially expressed transcripts (DETs). Twenty one DETs were selected for qPCR to validate the RNA-seq approach. The fold changes in gene expression identified by RNA-seq and qPCR were highly correlated (R-2 = 0.88). Several gene ontology terms including transcription factor activity and biological process such as glucose metabolic process were enriched among these DETs. Pathways involved in response to handling and confinement stress were implicated by mapping the DETs to reference pathways in the KEGG database. Accession Numbers: Raw RNA-seq reads have been submitted to the NCBI Short Read Archive under accession number SRP022881. Customized Perl Scripts: All customized scripts described in this paper are available from Dr. Guangtu Gao or the corresponding author. C1 [Liu, Sixin; Gao, Guangtu; Palti, Yniv; Cleveland, Beth M.; Weber, Gregory M.; Rexroad, Caird E., III] ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV USA. RP Rexroad, CE (reprint author), ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV USA. EM caird.rexroadiii@ars.usda.gov RI Gao, Guangtu/F-4541-2012 FU USDA/ARS National Center for Cool and Cold Water Aquaculture [1930-31000-009] FX This work was supported with base funding from the USDA/ARS National Center for Cool and Cold Water Aquaculture, Project 1930-31000-009. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 56 TC 11 Z9 11 U1 4 U2 35 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 18 PY 2014 VL 9 IS 2 AR e88492 DI 10.1371/journal.pone.0088492 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AB3RA UT WOS:000331706700041 PM 24558395 ER PT J AU Utsunomiya, YT Carmo, AS Neves, HHR Carvalheiro, R Matos, MC Zavarez, LB Ito, PKRK O'Brien, AMP Solkner, J Porto-Neto, LR Schenkel, FS McEwan, J Cole, JB da Silva, MVGB Van Tassell, CP Sonstegard, TS Garcia, JF AF Utsunomiya, Yuri T. Carmo, Adriana S. Neves, Haroldo H. R. Carvalheiro, Roberto Matos, Marcia C. Zavarez, Ludmilla B. Ito, Pier K. R. K. O'Brien, Ana M. Perez Soelkner, Johann Porto-Neto, Laercio R. Schenkel, Flavio S. McEwan, John Cole, John B. da Silva, Marcos V. G. B. Van Tassell, Curtis P. Sonstegard, Tad S. Garcia, Jose Fernando TI Genome-Wide Mapping of Loci Explaining Variance in Scrotal Circumference in Nellore Cattle SO PLOS ONE LA English DT Article ID ADDITIVE GENETIC-RELATIONSHIPS; PRADER-WILLI-SYNDROME; ASSOCIATION ANALYSIS; REPRODUCTIVE TRAITS; HEIFER PREGNANCY; BINDING-PROTEINS; GROWTH; SELECTION; PUBERTY; CLONING AB The reproductive performance of bulls has a high impact on the beef cattle industry. Scrotal circumference (SC) is the most recorded reproductive trait in beef herds, and is used as a major selection criterion to improve precocity and fertility. The characterization of genomic regions affecting SC can contribute to the identification of diagnostic markers for reproductive performance and uncover molecular mechanisms underlying complex aspects of bovine reproductive biology. In this paper, we report a genome-wide scan for chromosome segments explaining differences in SC, using data of 861 Nellore bulls (Bos indicus) genotyped for over 777,000 single nucleotide polymorphisms. Loci that excel from the genome background were identified on chromosomes 4, 6, 7, 10, 14, 18 and 21. The majority of these regions were previously found to be associated with reproductive and body size traits in cattle. The signal on chromosome 14 replicates the pleiotropic quantitative trait locus encompassing PLAG1 that affects male fertility in cattle and stature in several species. Based on intensive literature mining, SP4, MAGEL2, SH3RF2, PDE5A and SNAI2 are proposed as novel candidate genes for SC, as they affect growth and testicular size in other animal models. These findings contribute to linking reproductive phenotypes to gene functions, and may offer new insights on the molecular biology of male fertility. C1 [Utsunomiya, Yuri T.; Carmo, Adriana S.; Matos, Marcia C.; Zavarez, Ludmilla B.; Garcia, Jose Fernando] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Sao Paulo, Brazil. [Neves, Haroldo H. R.; Carvalheiro, Roberto] UNESP Univ Estadual Paulista, Dept Zootecnia, Sao Paulo, Brazil. [Carvalheiro, Roberto] GenSys Consultores Associados, Porto Alegre, RS, Brazil. [Ito, Pier K. R. K.; Garcia, Jose Fernando] UNESP Univ Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Saude & Prod Anim, Sao Paulo, Brazil. [Soelkner, Johann] BOKU Univ Nat Resources & Life Sci, Div Livestock Sci, Dept Sustainable Agr Syst, Vienna, Austria. [Porto-Neto, Laercio R.] CSIRO Commonwealth Sci & Ind Res Org, Food Futures Flagship, Brisbane, Qld, Australia. [Schenkel, Flavio S.] Univ Guelph, Ctr Genet Improvement Livestock, Guelph, ON N1G 2W1, Canada. [McEwan, John] AgResearch, Ctr Reprod & Genom, Mosgiel, Otago, New Zealand. [Cole, John B.] ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD USA. [da Silva, Marcos V. G. B.] Embrapa Dairy Cattle, Bioinformat & Anim Genom Lab, Juiz De Fora, MG, Brazil. [Sonstegard, Tad S.] ARS, Bovine Funct Genom Lab, USDA, Beltsville, MD USA. RP Garcia, JF (reprint author), UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Sao Paulo, Brazil. EM jfgarcia@fmva.unesp.br RI de Rezende Neves, Haroldo Henrique/F-9521-2012; Utsunomiya, Yuri Tani/K-5214-2013; Carmo, Andreia/D-8603-2012; Porto-Neto, Laercio/D-2594-2012; Cole, John/J-8571-2014; McEwan, John/A-1143-2008; OI Utsunomiya, Yuri Tani/0000-0002-6526-8337; Porto-Neto, Laercio/0000-0002-3536-8265; Cole, John/0000-0003-1242-4401; McEwan, John/0000-0003-4801-6207; Carvalheiro, Roberto/0000-0002-4506-0555; Van Tassell, Curtis/0000-0002-8416-2087 FU National Counsel of Technological and Scientific Development (CNPq) [560922/2010-8, 483590/2010-0]; Sao Paulo Research Foundation (FAPESP) [2011/16643-2, 2010/52030-2] FX This research was supported by: National Counsel of Technological and Scientific Development (CNPq - http://www.cnpq.br/) (process 560922/2010-8 and 483590/2010-0); and Sao Paulo Research Foundation (FAPESP - http://www.fapesp.br/) (process 2011/16643-2 and 2010/52030-2). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 6 Z9 6 U1 0 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 18 PY 2014 VL 9 IS 2 AR e88561 DI 10.1371/journal.pone.0088561 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AB3RA UT WOS:000331706700046 PM 24558400 ER PT J AU Wittmann, ME Cooke, RM Rothlisberger, JD Lodge, DM AF Wittmann, Marion E. Cooke, Roger M. Rothlisberger, John D. Lodge, David M. TI Using Structured Expert Judgment to Assess Invasive Species Prevention: Asian Carp and the Mississippi-Great Lakes Hydrologic Connection SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID RISK ANALYSIS; BIOLOGICAL INVASIONS; PROPAGULE PRESSURE; FRAMEWORK; PATHWAYS; POLICY AB Recently, authors have theorized that invasive species prevention is more cost-effective than control in protecting ecosystem services. However, quantification of the effectiveness of prevention is rare because experiments at field scales are expensive or infeasible. We therefore used structured expert judgment to quantify the efficacy of 17 proposed strategies to prevent Asian carp invasion of the Laurentian Great Lakes via the hydrologic connection between the Mississippi and Great Lakes watersheds. Performance-weighted expert estimates indicated that hydrologic separation would prevent 99% (95,100; median, 5th and 95th percentiles) of Asian carp access, while electric and acoustic-bubble-strobe barriers would prevent 92% (85,95) and 92% (75,95), respectively. For all other strategies, estimated effectiveness was lower, with greater uncertainty. When potential invasions by other taxa are considered, the effectiveness of hydrologic separation increases relative to strategies that are effective primarily for fishes. These results could help guide invasive species management in many waterways globally. C1 [Wittmann, Marion E.; Lodge, David M.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. [Cooke, Roger M.] Univ Strathclyde, Tech Univ Delft, Resources Future, Glasgow, Lanark, Scotland. [Rothlisberger, John D.] US Forest Serv, Milwaukee, WI 53202 USA. [Lodge, David M.] Univ Notre Dame, Environm Change Initiat, Notre Dame, IN 46556 USA. RP Wittmann, ME (reprint author), Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. EM mwittmann@gmail.com FU Environmental Protection Agency Great Lakes Restoration Initiative, NOAA Center for Sponsored Coastal Ocean Research [NA09NOS4780192, NA10NOS4780218] FX This research was funded by Environmental Protection Agency Great Lakes Restoration Initiative, NOAA Center for Sponsored Coastal Ocean Research: NA09NOS4780192, NA10NOS4780218. We thank each expert for their thoughtful participation and S. Chandra, A. Deines, C. Jerde, and M. Marchetti for their valuable review and input. This is a publication of the Notre Dame Environmental Change Initiative. NR 45 TC 12 Z9 13 U1 14 U2 120 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD FEB 18 PY 2014 VL 48 IS 4 BP 2150 EP 2156 DI 10.1021/es4043098 PG 7 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AB4QD UT WOS:000331774100008 PM 24467555 ER PT J AU Susanti, D Wong, JH Vensel, WH Loganathan, U DeSantis, R Schmitz, RA Balsera, M Buchanan, BB Mukhopadhyay, B AF Susanti, Dwi Wong, Joshua H. Vensel, William H. Loganathan, Usha DeSantis, Rebecca Schmitz, Ruth A. Balsera, Monica Buchanan, Bob B. Mukhopadhyay, Biswarup TI Thioredoxin targets fundamental processes in a methane-producing archaeon, Methanocaldococcus jannaschii SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE methanogenic archaea; redox regulation; hydrothermal vent; early Earth; evolution ID PROTEIN-DISULFIDE OXIDOREDUCTASE; METHANOCOCCUS-JANNASCHII; METHANOBACTERIUM-THERMOAUTOTROPHICUM; METHANOGENIC ARCHAEA; COENZYME F-420; SP-NOV; REDUCTASE; IDENTIFICATION; DEHYDROGENASE; PURIFICATION AB Thioredoxin (Trx), a small redox protein, controls multiple processes in eukaryotes and bacteria by changing the thiol redox status of selected proteins. The function of Trx in archaea is, however, unexplored. To help fill this gap, we have investigated this aspect in methanarchaea-strict anaerobes that produce methane, a fuel and greenhouse gas. Bioinformatic analyses suggested that Trx is nearly universal in methanogens. Ancient methanogens that produce methane almost exclusively from H-2 plus CO2 carried approximately two Trx homologs, whereas nutritionally versatile members possessed four to eight. Due to its simplicity, we studied the Trx system of Methanocaldococcus jannaschii-a deeply rooted hyperthermophilic methanogen growing only on H-2 plus CO2. The organism carried two Trx homologs, canonical Trx1 that reduced insulin and accepted electrons from Escherichia coli thioredoxin reductase and atypical Trx2. Proteomic analyses with air-oxidized extracts treated with reduced Trx1 revealed 152 potential targets representing a range of processes-including methanogenesis, biosynthesis, transcription, translation, and oxidative response. In enzyme assays, Trx1 activated two selected targets following partial deactivation by O-2, validating proteomics observations: methylenetetrahydromethanopterin dehydrogenase, a methanogenesis enzyme, and sulfite reductase, a detoxification enzyme. The results suggest that Trx assists methanogens in combating oxidative stress and synchronizing metabolic activities with availability of reductant, making it a critical factor in the global carbon cycle and methane emission. Because methanogenesis developed before the oxygenation of Earth, it seems possible that Trx functioned originally in metabolic regulation independently of O2, thus raising the question whether a complex biological system of this type evolved at least 2.5 billion years ago. C1 [Susanti, Dwi; Loganathan, Usha; Mukhopadhyay, Biswarup] Virginia Tech, Dept Biochem, Blacksburg, VA 24061 USA. [Loganathan, Usha; Mukhopadhyay, Biswarup] Virginia Tech, Dept Biol Sci, Blacksburg, VA 24061 USA. [Susanti, Dwi] Virginia Tech, Dept Genet, Bioinformat & Computat Biol Grad Program, Blacksburg, VA 24061 USA. [Susanti, Dwi; Loganathan, Usha; Mukhopadhyay, Biswarup] Virginia Tech, Virginia Bioinformat Inst, Blacksburg, VA 24061 USA. [Wong, Joshua H.; Buchanan, Bob B.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Vensel, William H.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [DeSantis, Rebecca; Schmitz, Ruth A.] Univ Kiel, Inst Allgemeine Mikrobiol, D-24118 Kiel, Germany. [Balsera, Monica] Inst Recursos Nat & Agrobiol Salamanca IRNASA CSI, Dept Estres Abiot, Salamanca 37008, Spain. RP Buchanan, BB (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. EM view@berkeley.edu; biswarup@vt.edu RI Balsera, Monica/K-2877-2014; OI Balsera, Monica/0000-0002-5586-6050; Schmitz-Streit, Ruth Anne/0000-0002-6788-0829 FU National Science Foundation [MCB 1020458]; National Aeronautics and Space Administration [NNX13AI05G]; US Department of Agriculture Agricultural Research Service Current Research Information System Project [5325-43000-026-00]; Genetics, Bioinformatics, and Computational Biology Graduate Program FX This work was supported by National Science Foundation Grant MCB 1020458 (to B. M. and B. B. B.) and National Aeronautics and Space Administration Astrobiology: Exobiology and Evolutionary Biology Grant NNX13AI05G (to B. M.). The mass spectrometric analysis was supported by the US Department of Agriculture Agricultural Research Service Current Research Information System Project 5325-43000-026-00. D. S. was partially supported by a fellowship from the Genetics, Bioinformatics, and Computational Biology Graduate Program. We thank Dr. David Grahame (Uniformed Services University of the Health Sciences) for a gift of tetrahydrosarcinapterin and Dr. William Whitman (University of Georgia) for suggesting an evolutionary implication of our observations. NR 53 TC 12 Z9 13 U1 2 U2 28 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 18 PY 2014 VL 111 IS 7 BP 2608 EP 2613 DI 10.1073/pnas.1324240111 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AA9EL UT WOS:000331396500047 PM 24505058 ER PT J AU Masi, M Meyer, S Clement, S Andolfi, A Cimmino, A Evidente, A AF Masi, Marco Meyer, Susan Clement, Suzette Andolfi, Anna Cimmino, Alessio Evidente, Antonio TI Spirostaphylotrichin W, a spirocyclic gamma-lactam isolated from liquid culture of Pyrenophora semeniperda, a potential mycoherbicide for cheatgrass (Bromus tectorum) biocontrol SO TETRAHEDRON LA English DT Article DE Bromus tectorum; Biocontrol; Pyrenophora semeniperda; Spirocyclic gamma-lactam; Spirostaphylotrichin W ID DRECHSLERA-TRITICI-REPENTIS; STAPHYLOTRICHUM-COCCOSPORUM; METABOLITES; PATHOGEN; GROWTH AB A novel spirocyclic gamma-lactam, named spirostaphylotrichin W (1), was isolated together with the well known and closely related spirostaphylotrichins A, C, D, R and V, as well as triticone E, from the liquid cultures of Pyrenophora semeniperda (anamorph: Drechslera), a seed pathogen proposed for cheatgrass (Bromus tectorum) biocontrol. Spirostaphylotrichin W was characterized as (3S*,4S*,5S*,6S*,9Z,10Z)-4,6-dihydroxy-2,3-dimethoxy-3-methyl-10-propyliden-2-azaspiro [4.5]dec-8-ene-1,7-dione, by spectroscopic and chemical methods. The relative stereochemistry of spirostaphylotrichin W was assigned using NOESY experiments and in comparison to those of spirostaphylotrichin V (2) and triticone E (6). In fact, the relative stereochemistry at C-3 was the same of that of 2, while that at C-4 and C-6 was inverted in respect to that reported, respectively, for 2 and 6. In a B. tectorum coleoptile bioassay at concentration of 10-3, spirostaphylotrichin A proved to be the most active compound, followed by spirostaphylotrichins C and D. Spirostaphylotrichin W and V showed mild toxicity while spirostaphylotrichin R and triticone E were not active. When tested on host and non-host plants by leaf puncture bioassay, spirostaphylotrichins A, C and D caused the appearance of necrotic spots while the other compounds were inactive. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Masi, Marco; Andolfi, Anna; Cimmino, Alessio; Evidente, Antonio] Univ Naples Federico II, Dipartimento Sci Chim, I-80126 Naples, Italy. [Masi, Marco] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84601 USA. [Meyer, Susan; Clement, Suzette] US Forest Serv, Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. RP Evidente, A (reprint author), Univ Naples Federico II, Dipartimento Sci Chim, Complesso Univ Monte St Angelo,Via Cintia 4, I-80126 Naples, Italy. EM evidente@unina.it RI Evidente, Antonio/N-9357-2013; Masi, Marco/J-7742-2016; Cimmino, Alessio/I-2121-2012 OI Evidente, Antonio/0000-0001-9110-1656; Masi, Marco/0000-0003-0609-8902; Cimmino, Alessio/0000-0002-1551-4237 NR 17 TC 6 Z9 6 U1 1 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0040-4020 J9 TETRAHEDRON JI Tetrahedron PD FEB 18 PY 2014 VL 70 IS 7 BP 1497 EP 1501 DI 10.1016/j.tet.2013.12.056 PG 5 WC Chemistry, Organic SC Chemistry GA AA3VQ UT WOS:000331023700016 ER PT J AU Wang, DP Tian, P AF Wang, Dapeng Tian, Peng TI Inactivation conditions for human norovirus measured by an in situ capture-qRT-PCR method SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Human norovirus; HBGA-capture qRT-PCR; Encapsidated viral RNA; Inactivation; In situ capture qRT-PCR (ISC-qRT-PCR) ID PORCINE GASTRIC MUCIN; MURINE NOROVIRUS; FELINE-CALICIVIRUS; HEAT INACTIVATION; NORWALK VIRUS; SURROGATE; WATER; ACID; MS2; UV AB Human norovirus (HuNoV) is a leading cause of foodborne gastroenteritis. Unfortunately, the inactivation parameters for HuNoV in clinical, food and environmental samples have not been established. Due to the inability to cultivate HuNoV in vitro, quantitative real-time RT-PCR (qRT-PCR) is widely-used for detecting HuNoVs. However, qRT-PCR does not indicate viral infectivity. Our method employs histo-blood group antigens (HBGAs) as viral receptors/co-receptors and container-affixed capture agents to concentrate HuNoVs. The captured viruses are denatured and its genome is amplified in the same module by in situ capture qRT-PCR (ISC-qRT-PCR). Greater than three log(10) reduction in the receptor-captured viral genomic signal (RCVGS) was observed when HuNoV was treated by heat at 72 degrees C for 4 min, by chlorine at a final concentration of 16 ppm in less than 1 min, and by UV irradiation at 1 J/cm(2). Treatment of low-titer HuNoV (<10(3) copies/sample) with 70% ethanol for 20 s reduced the RCVGS of HuNoV by two log(10). However, ethanol had a limited effect on high-titer samples of HuNoV (>10(3) copies/sample). The results demonstrate that ISC-qRT-PCR method could be used as an alternative method to measure encapsidated viral RNA and indirectly indicate the inactivation status of HuNoV caused by physical treatment such as heat, and chemical treatment such as chlorine, that damage the ability of the virus to bind to its receptor. Published by Elsevier B.V. C1 [Wang, Dapeng] Shanghai Jiao Tong Univ, Sch Agr & Biol, Bor Luh Food Safety Ctr, MOST USDA Joint Res Ctr Food Safety,State Key Lab, Shanghai 200240, Peoples R China. [Wang, Dapeng; Tian, Peng] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Tian, P (reprint author), ARS, WRRC, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM peng.tian@ars.usda.gov FU Ministry of Science and Technology of China [2012AA101601]; National Natural Science Foundation of China [31000063]; USDA Agricultural Research Service CRIS project [5325-42000-046-06R] FX This work was jointly supported by the Ministry of Science and Technology of China (Grant # 2012AA101601), National Natural Science Foundation of China (Grant # 31000063) and USDA Agricultural Research Service CRIS project 5325-42000-046-06R. USDA is an equal opportunity provider and employer. We want to thank David Yang (PSMRU, WRRC, ARS, USDA) for providing help for experiments and manuscript preparation. NR 33 TC 10 Z9 11 U1 0 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD FEB 17 PY 2014 VL 172 BP 76 EP 82 DI 10.1016/j.ijfoodmicro.2013.11.027 PG 7 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA AA8MM UT WOS:000331349600011 PM 24361836 ER PT J AU Corby-Harris, V Jones, BM Walton, A Schwan, MR Anderson, KE AF Corby-Harris, Vanessa Jones, Beryl M. Walton, Alexander Schwan, Melissa R. Anderson, Kirk E. TI Transcriptional markers of sub-optimal nutrition in developing Apis mellifera nurse workers SO BMC GENOMICS LA English DT Article DE Transcriptome; Apis mellifera; Nutrition; Starvation; Nurse; Development; Physiology ID DIFFERENTIAL EXPRESSION ANALYSIS; BEE COLONY LOSSES; DIVISION-OF-LABOR; HONEY-BEE; JUVENILE-HORMONE; GENE-EXPRESSION; RNA-SEQ; ENRICHMENT ANALYSIS; DIET; AGE AB Background: Honey bees (Apis mellifera) contribute substantially to the worldwide economy and ecosystem health as pollinators. Pollen is essential to the bee's diet, providing protein, lipids, and micronutrients. The dramatic shifts in physiology, anatomy, and behavior that accompany normal worker development are highly plastic and recent work demonstrates that development, particularly the transition from nurse to foraging roles, is greatly impacted by diet. However, the role that diet plays in the developmental transition of newly eclosed bees to nurse workers is poorly understood. To further understand honey bee nutrition and the role of diet in nurse development, we used a high-throughput screen of the transcriptome of 3 day and 8 day old worker bees fed either honey and stored pollen (rich diet) or honey alone (poor diet) within the hive. We employed a three factor (age, diet, age x diet) analysis of the transcriptome to determine whether diet affected nurse worker physiology and whether poor diet altered the developmental processes normally associated with aging. Results: Substantial changes in gene expression occurred due to starvation. Diet-induced changes in gene transcription occurring in younger bees were largely a subset of those occurring in older bees, but certain signatures of starvation were only evident 8 day old workers. Of the 18,542 annotated transcripts in the A. mellifera genome, 150 transcripts exhibited differential expression due to poor diet at 3d of age compared with 17,226 transcripts that differed due to poor diet at 8d of age, and poor diet caused more frequent down-regulation of gene expression in younger bees compared to older bees. In addition, the age-related physiological changes that accompanied early adult development differed due to the diet these young adult bees were fed. More frequent down-regulation of gene expression was observed in developing bees fed a poor diet compared to those fed an adequate diet. Functional analyses also suggest that the physiological and developmental processes occurring in well-fed bees are vastly different than those occurring in pollen deprived bees. Our data support the hypothesis that poor diet causes normal age-related development to go awry. Conclusion: Poor nutrition has major consequences for the expression of genes underlying the physiology and age-related development of nurse worker bees. More work is certainly needed to fully understand the consequences of starvation and the complex biology of nutrition and development in this system, but the genes identified in the present study provide a starting point for understanding the consequences of poor diet and for mitigating the economic costs of colony starvation. C1 [Corby-Harris, Vanessa; Jones, Beryl M.; Walton, Alexander; Schwan, Melissa R.; Anderson, Kirk E.] USDA ARS, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. [Corby-Harris, Vanessa; Anderson, Kirk E.] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. RP Corby-Harris, V (reprint author), USDA ARS, Carl Hayden Bee Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. EM v.corbyharris@gmail.com NR 55 TC 3 Z9 3 U1 1 U2 34 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 15 PY 2014 VL 15 AR 134 DI 10.1186/1471-2164-15-134 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AC5ZW UT WOS:000332600600001 PM 24529032 ER PT J AU Paraginski, RT Vanier, NL Moomand, K de Oliveira, M Zavareze, ED Silva, RME Ferreira, CD Elias, MC AF Paraginski, Ricardo Tadeu Vanier, Nathan Levien Moomand, Khalid de Oliveira, Mauricio Zavareze, Elessandra da Rosa Marques e Silva, Ricardo Ferreira, Cristiano Dietrich Elias, Moacir Cardoso TI Characteristics of starch isolated from maize as a function of grain storage temperature SO CARBOHYDRATE POLYMERS LA English DT Article DE Maize; Storage temperature; Starch; Pasting properties; Crystallinity ID CORN STARCHES; RHEOLOGICAL PROPERTIES; DRYING TEMPERATURE; THERMAL-PROPERTIES; PASTING PROPERTIES; RICE GRAINS; FLOUR; GELATINIZATION; DIGESTIBILITY; PROTEINS AB Considering the importance of maize starch and the lack of knowledge about the effects of storage temperature on the isolated starch properties; maize grains were stored during 12 months at different temperatures (5, 15,25 and 35 degrees C). The extraction yield and the physicochemical, thermal, pasting, crystallinity and morphological properties of starches were determined. The starch isolated from grains stored at 35 C was yellowish and showed a 22.1% decrease in starch extraction yield compared to freshly harvested maize grains. At 35 degrees C, a reduction in crystallinity was observed by the end of 12 months, despite a parallel rearrangement of the starch chains which resulted in an increase in X-ray peak intensities, gelatinisation temperatures and enthalpy. The starch isolated from maize grains stored at 35 degrees C appears to have smaller granules, which presents some points in their surface, potentially attributed to the protein matrix compressing the granules within maize grains. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Paraginski, Ricardo Tadeu; Vanier, Nathan Levien; de Oliveira, Mauricio; Zavareze, Elessandra da Rosa; Ferreira, Cristiano Dietrich; Elias, Moacir Cardoso] Univ Fed Pelotas, Dept Agroind Sci & Technol, BR-96010900 Pelotas, RS, Brazil. [Vanier, Nathan Levien] USDA ARS, Proc Foods Res Unit, WRRC, Albany, CA 94710 USA. [Moomand, Khalid] Univ Guelph, Dept Food Sci, Guelph, ON N1G 2W1, Canada. [Marques e Silva, Ricardo] Univ Fed Pelotas, Dept Electron Microscopy, BR-96015560 Pelotas, RS, Brazil. RP Paraginski, RT (reprint author), Univ Fed Pelotas, Dept Agroind Sci & Technol, BR-96010900 Pelotas, RS, Brazil. EM paraginskiricardo@yahoo.com.br OI Vanier, Nathan/0000-0001-6592-3023 FU CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior); CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico); SCT-RS (Secretaria da Ciencia e Tecnologia do Estado do Rio Grande do Sul); Polo de Inovacao Tecnologica em Alimentos da Regiao Sul FX We would like to thank CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior), CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), SCT-RS (Secretaria da Ciencia e Tecnologia do Estado do Rio Grande do Sul) and Polo de Inovacao Tecnologica em Alimentos da Regiao Sul. NR 35 TC 8 Z9 8 U1 1 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD FEB 15 PY 2014 VL 102 BP 88 EP 94 DI 10.1016/j.carbpol.2013.11.019 PG 7 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA AB4SG UT WOS:000331779600012 PM 24507259 ER PT J AU Chen, M Mishra, S Heckathorn, SA Frantz, JM Krause, C AF Chen, Mei Mishra, Sasmita Heckathorn, Scott A. Frantz, Jonathan M. Krause, Charles TI Proteomic analysis of Arabidopsis thaliana leaves in response to acute boron deficiency and toxicity reveals effects on photosynthesis, carbohydrate metabolism, and protein synthesis SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE B-deficiency; B-toxicity; Photosynthesis ID MEMBRANE-PERMEABILITY; SUNFLOWER LEAVES; BARLEY CULTIVARS; OXIDATIVE STRESS; PHOTOSYSTEM-II; PLANTS; TOLERANCE; TOBACCO; WHEAT; ANTIOXIDANTS AB Boron (B) stress (deficiency and toxicity) is common in plants, but as the functions of this essential micronutrient are incompletely understood, so too are the effects of B stress. To investigate mechanisms underlying B stress, we examined protein profiles in leaves of Arabidopsis thaliana plants grown under normal B (30 mu M), compared to plants transferred for 60 and 84 h (i.e., before and after initial visible symptoms) in deficient (0 mu M) or toxic (3 mM) levels of B. B-responsive polypeptides were sequenced by mass spectrometry, following 2D gel electrophoresis, and 1D gels and immunoblotting were used to confirm the B-responsiveness of some of these proteins. Fourteen B-responsive proteins were identified, including: 9 chloroplast proteins, 6 proteins of photosynthetic/carbohydrate metabolism (rubisco activase, OEC23, photosystem I reaction center subunit II-1, ATPase 8-subunit, glycolate oxidase, fructose bisphosphate aldolase), 6 stress proteins, and 3 proteins involved in protein synthesis (note that the 14 proteins may fall into multiple categories). Most (8) of the B-responsive proteins decreased under both B deficiency and toxicity; only 3 increased with B stress. Boron stress decreased, or had no effect on, 3 of 4 oxidative stress proteins examined, and did not affect total protein. Hence, our results indicate relatively early specific effects of B stress on chloroplasts and protein synthesis. (C) 2013 Elsevier GmbH. All rights reserved. C1 [Chen, Mei; Mishra, Sasmita; Heckathorn, Scott A.] Univ Toledo, Dept Environm Sci, Toledo, OH 43606 USA. [Frantz, Jonathan M.; Krause, Charles] Univ Toledo, USDA ARS, Toledo, OH 43606 USA. RP Mishra, S (reprint author), Univ Toledo, Dept Environm Sci, MS 604, Toledo, OH 43606 USA. EM sasmita.mishra@utoledo.edu FU United States Department of Agriculture, Agricultural Research Service [SCA 58-3607-4-119] FX This research was supported by the United States Department of Agriculture, Agricultural Research Service (SCA 58-3607-4-119 to J. Gray and S.A. Heckathorn). The authors thank Drs. Tzann-Wei Wang, Archie Portis, Edith Camm, and Ann Eastman for antisera to specific proteins, and the personnel at the Campus Chemical Instrument Center of Ohio State University for help with the mass spectrometry. NR 60 TC 11 Z9 16 U1 3 U2 38 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD FEB 15 PY 2014 VL 171 IS 3-4 BP 235 EP 242 DI 10.1016/j.jplph.2013.07.008 PG 8 WC Plant Sciences SC Plant Sciences GA AB8PJ UT WOS:000332052200006 PM 23988561 ER PT J AU Chen, GY Chen, TW AF Chen, Guoying Chen, Tuanwei TI SPE speciation of inorganic arsenic in rice followed by hydride-generation atomic fluorescence spectrometric quantification SO TALANTA LA English DT Article DE Inorganic arsenic; Rice; SPE; Speciation; Hydride generation; Atomic fluorescence spectrometry ID PLASMA-MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; ABSORPTION-SPECTROMETRY; ION-EXCHANGE; CHROMATOGRAPHY; CARTRIDGES; SEPARATION; STABILITY; INJECTION; PRODUCTS AB Due to high toxicity, inorganic arsenic (iAs) species are the focus of monitoring effort worldwide. In this work arsenic was first extracted from rice by microwave-assisted digestion in HNO3-H2O2, during which As-III was oxidized to As-V. Silica-based strong anion exchange cartridges were used to separate As-V from organic forms. After prereduction by iodide, iAs was quantified by hydride-generation atomic fluorescence spectrometry (HG-AFS). This method achieved 1.3 ng g(-1) limit of detection (LOD), and 94 +/- 3% and 93 +/- 5% recoveries, respectively, for As-III and As-V at 100 ng g(-1). Validation was performed using standard reference material NIST 1568a (102 ng g(-1)) and ERM BC211 (124 ng g(-1)) rice flour. By eliminating chromatography, SPE speciation gained throughput and cost advantages. HG-AFS, at 10% budget and operation cost of a typical inductively-couple plasma mass spectrometer (ICPMS), proved highly sensitive and specific for iAs quantification. Published by Elsevier B.V. C1 [Chen, Guoying] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Chen, Tuanwei] Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Fujian, Peoples R China. RP Chen, GY (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM guoying.chen@ars.usda.gov RI Chen, Guoying/C-6800-2015 OI Chen, Guoying/0000-0001-9532-0696 NR 35 TC 23 Z9 23 U1 5 U2 69 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0039-9140 EI 1873-3573 J9 TALANTA JI Talanta PD FEB 15 PY 2014 VL 119 BP 202 EP 206 DI 10.1016/j.talanta.2013.11.016 PG 5 WC Chemistry, Analytical SC Chemistry GA AB3DE UT WOS:000331670700029 PM 24401405 ER PT J AU Kreye, JK Brewer, NW Morgan, P Varner, JM Smith, AMS Hoffman, CM Ottmar, RD AF Kreye, Jesse K. Brewer, Nolan W. Morgan, Penelope Varner, J. Morgan Smith, Alistair M. S. Hoffman, Chad M. Ottmar, Roger D. TI Fire behavior in masticated fuels: A review SO FOREST ECOLOGY AND MANAGEMENT LA English DT Review DE Combustion; Fire hazard; Fuels heterogeneity; Fuels management; Fuels treatments; Silviculture ID WILDLAND-URBAN INTERFACE; COARSE WOODY DEBRIS; REDUCTION TREATMENTS; MOISTURE-CONTENT; ATMOSPHERIC INTERACTIONS; MECHANICAL MASTICATION; UNITED-STATES; NATIONAL FIRE; FOREST; SURFACE AB Mastication is an increasingly common fuels treatment that redistributes "ladder" fuels to the forest floor to reduce vertical fuel continuity, crown fire potential, and fireline intensity, but fuel models do not exist for predicting fire behavior in these fuel types. Recent fires burning in masticated fuels have behaved in unexpected and contradictory ways, likely because the shredded, compact fuel created when trees and shrubs are masticated contains irregularly shaped pieces in mixtures quite different from other woody fuels. We review fuels characteristics and fire behavior in masticated fuels across the United States. With insights from the few laboratory and field burning experiments conducted, we highlight the variation likely to occur across different ecosystems in which these treatments are being widely implemented. Masticated debris has a propensity to flame and smolder for long durations. Fuel variability and vegetation response will likely influence whether or not treatments reduce long-term fire hazard. We identify key science needs that will better elucidate fire behavior and effects in these treatments. With mastication widely applied in an expanding wildland-urban interface it is crucial to understand how such fuels burn. What we learn about combustion in these fuels will inform effective fuels management in these and other mixed fuels. (C) 2013 Elsevier B.V. All rights reserved. C1 [Kreye, Jesse K.; Varner, J. Morgan] Mississippi State Univ, Forest & Wildlife Res Ctr, Dept Forestry, Mississippi State, MS 39762 USA. [Brewer, Nolan W.; Morgan, Penelope; Smith, Alistair M. S.] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. [Brewer, Nolan W.] Washington State Dept Nat Resources, Colville, WA 99114 USA. [Hoffman, Chad M.] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. [Ottmar, Roger D.] US Forest Serv, Pacific NW Res Stn, Pacific Wildland Fire Sci Lab, Seattle, WA 98103 USA. RP Morgan, P (reprint author), Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. EM jkreye@cfr.msstate.edu; Nolan.brewer@dnr.wa.gov; pmorgan@uidaho.edu; mvarner@cfr.msstate.edu; alistair@uidaho.edu; c.hoffman@colostate.edu; rottmar@fs.fed.us RI Smith, Alistair/I-3162-2014; OI Smith, Alistair/0000-0003-0071-9958; Hoffman, Chad/0000-0001-8715-937X FU US Department of Agriculture, Rocky Mountain Research Station [08-JV-11221611-160]; US Department of Agriculture; US Department of Interior Joint Fire Science Program [13-1-05-7, 12-1-03-31]; University of Idaho FX A synthesis necessarily draws on research of others; we acknowledge the input and informal contributions of E.E. Knapp, J.M. Kane, and L.N. Kobziar to this review. This research was supported in part by funds provided by the US Department of Agriculture, Rocky Mountain Research Station (08-JV-11221611-160), by the US Department of Agriculture and US Department of Interior Joint Fire Science Program (Projects 13-1-05-7 and 12-1-03-31), and the University of Idaho. NR 92 TC 17 Z9 17 U1 2 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 15 PY 2014 VL 314 BP 193 EP 207 DI 10.1016/j.foreco.2013.11.035 PG 15 WC Forestry SC Forestry GA AB5TH UT WOS:000331851100021 ER PT J AU Mandel, T Moreau, F Kutsher, Y Fletcher, JC Carles, CC Williams, LE AF Mandel, Tali Moreau, Fanny Kutsher, Yaarit Fletcher, Jennifer C. Carles, Cristel C. Williams, Leor Eshed TI The ERECTA receptor kinase regulates Arabidopsis shoot apical meristem size, phyllotaxy and floral meristem identity SO DEVELOPMENT LA English DT Article DE AGAMOUS (AG); Cell fate; WUSCHEL (WUS) ID LATERAL ORGAN DEVELOPMENT; STEM-CELL MAINTENANCE; MADS-BOX GENES; FLOWER DEVELOPMENT; HOMEOTIC GENE; AUXIN-TRANSPORT; SIGNALING PATHWAY; PLANT DEVELOPMENT; THALIANA; EXPRESSION AB In plants, the shoot apical meristem (SAM) serves as a reservoir of pluripotent stem cells from which all above ground organs originate. To sustain proper growth, the SAM must maintain homeostasis between the self-renewal of pluripotent stem cells and cell recruitment for lateral organ formation. At the core of the network that regulates this homeostasis in Arabidopsis are the WUSCHEL (WUS) transcription factor specifying stem cell fate and the CLAVATA (CLV) ligand-receptor system limiting WUS expression. In this study, we identified the ERECTA (ER) pathway as a second receptor kinase signaling pathway that regulates WUS expression, and therefore shoot apical and floral meristem size, independently of the CLV pathway. We demonstrate that reduction in class III HD-ZIP and ER function together leads to a significant increase in WUS expression, resulting in extremely enlarged shoot meristems and a switch from spiral to whorled vegetative phyllotaxy. We further show that strong upregulation of WUS in the inflorescence meristem leads to ectopic expression of the AGAMOUS homeotic gene to a level that switches cell fate from floral meristem founder cell to carpel founder cell, suggesting an indirect role for ER in regulating floral meristem identity. This work illustrates the delicate balance between stem cell specification and differentiation in the meristem and shows that a shift in this balance leads to abnormal phyllotaxy and to altered reproductive cell fate. C1 [Mandel, Tali; Kutsher, Yaarit; Williams, Leor Eshed] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, Israel. [Moreau, Fanny; Carles, Cristel C.] CNRS, UMR5168, F-38054 Grenoble, France. [Fletcher, Jennifer C.] Univ Calif Berkeley, USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. RP Williams, LE (reprint author), Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, POB 12, IL-76100 Rehovot, Israel. EM leor.williams@mail.huji.ac.il FU Israel Science Foundation [1351/10]; Vaadia-BARD [IS-4336-10R]; US Department of Agriculture [5335-21000-029-00D]; Centre National de la Recherche Scientifique (CNRS Higher Education chair) [0428-64]; Alpes county [ADR Cluster] [12-01293101] FX This work was supported by the Israel Science Foundation [1351/10 to T. M.], by Vaadia-BARD [IS-4336-10R to Y.K.], by the US Department of Agriculture [5335-21000-029-00D to J.C.F], by the Centre National de la Recherche Scientifique (CNRS Higher Education chair [position 0428-64 to C.C.C.] and by Alpes county [ADR Cluster 7, 12-01293101 to F.M.]. Deposited in PMC for immediate release. NR 82 TC 20 Z9 21 U1 3 U2 50 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND SN 0950-1991 EI 1477-9129 J9 DEVELOPMENT JI Development PD FEB 15 PY 2014 VL 141 IS 4 BP 830 EP 841 DI 10.1242/dev.104687 PG 12 WC Developmental Biology SC Developmental Biology GA AB0DB UT WOS:000331460900010 PM 24496620 ER PT J AU Williams, MM AF Williams, Martin M., II TI Few crop traits accurately predict variables important to productivity of processing sweet corn SO FIELD CROPS RESEARCH LA English DT Article DE Economic analysis; Food processor; Industrial yield; Research methods; Yield estimate ID PLANTING DATE; YIELD; INTERFERENCE; PROLIFICACY; MAIZE; MANAGEMENT; HYBRIDS; GROWTH; FEASIBILITY; PERFORMANCE AB Recovery, case production, and gross profit margin, hereafter called 'processor variables', are as important metrics to processing sweet corn as grain yield is to field corn production. However, crop traits such as ear number or ear mass alone are reported in sweet corn production research rather than processor variables. The objective of this research was to determine the extent to which certain crop traits could be used to predict variables important to productivity of sweet corn grown for processing. The data used in this research reflected 22 different growing environments over an 8-year period representing 31 processing hybrids. Relations between processor variables and 17 crop traits (5 plant traits, 8 ear traits, and 4 yield traits) were characterized. None of the crop traits adequately predicted recovery, defined as the percentage of green ear mass (i.e. complete ears with husk leaves) represented by fresh kernel mass. Case production, defined as cases of kernels per unit area, was strongly associated (rho >= 0.869) with ear number, green ear mass, husked ear mass, and fresh kernel mass. Similar correlations (rho >= 0.854) were found between the yield traits and gross profit margin, defined as the value of case production less the contracted cost of green ear mass. However, regression analyses of relationships between processor variables and individual yield traits showed that fresh kernel mass was by far the best predictor of case production and gross profit margin. While ear number or green ear mass are commonly reported in field research of processing sweet corn, relevancy of the research would be enhanced if fresh kernel mass were measured and reported. Published by Elsevier B.V. C1 Univ Illinois, USDA ARS, Urbana, IL 67801 USA. RP Williams, MM (reprint author), Univ Illinois, USDA ARS, Urbana, IL 67801 USA. EM mmwillms@illinois.edu NR 25 TC 2 Z9 2 U1 2 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD FEB 15 PY 2014 VL 157 BP 20 EP 26 DI 10.1016/j.fcr.2013.12.003 PG 7 WC Agronomy SC Agriculture GA AB0TC UT WOS:000331504000003 ER PT J AU Duan, YH Xu, MG Gao, SD Yang, XY Huang, SM Liu, HB Wang, BR AF Duan, Yinghua Xu, Minggang Gao, Suduan Yang, Xueyun Huang, Shaomin Liu, Hongbin Wang, Bairen TI Nitrogen use efficiency in a wheat-corn cropping system from 15 years of manure and fertilizer applications SO FIELD CROPS RESEARCH LA English DT Article DE Fertilizer contribution; Long-term fertilization; Phosphorus; Potassium; Stover return ID INORGANIC FERTILIZER; PHOSPHORUS; SOIL; YIELDS; CHINA; RICE; TEMPERATURE; MANAGEMENT; RESPONSES; REGIMES AB Low nitrogen use efficiency (NUE) and adverse environmental impacts caused by N fertilization increasingly threaten the sustainability of agriculture. To develop strategies for efficient nutrient management, we investigated the effects of long-term (1991-2005) various fertilization regimes on yield, NUE and N agronomic efficiency (NAE) in a wheat-corn cropping system at four sites (Changping, Zhengzhou, Yangling, and Qiyang) in China. Treatments included unfertilized control (CK), chemical fertilization only (N, NP, NK, and NPK), manure application (supplying 70% N) with NPK (NPKm) and at 1.5x rate (1.5NPKm), and NPK with corn stover returned (NPKs). The NP and NPK treatments resulted in generally higher yield, NUE and NAE than the N and NK for both wheat and corn demonstrating the importance of P in NUE improvement. The manure treatments resulted in significant increase or no decrease in the overall system NUE in all four sites with the highest mean NUE (49%) from NPKm treatment. In acid soil at Qiyang, only manure treatments resulted in significant increases of NUE and NAE indicating the importance of organic amendment. Wheat was more responsive to P fertilizer and corn was more responsive to manure in NUE improvement. Thus an effective nutrient management strategy is to ensure adequate P supply for the wheat crop and manure application for the corn crop to improve overall NUE for the wheat-corn production system. (C) 2013 Elsevier B.V. All rights reserved. C1 [Duan, Yinghua; Xu, Minggang; Liu, Hongbin; Wang, Bairen] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Crop Nutr & Fertilizat, Minist Agr China, Beijing 100081, Peoples R China. [Gao, Suduan] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Yang, Xueyun] Northwest Sci Tech Univ Agr & Forestry, Coll Resource & Environm, Yangling 712100, Shaanxi, Peoples R China. [Huang, Shaomin] Henan Acad Agr Sci, Inst Plant Nutr & Agr Resources, Zhengzhou 450002, Henan, Peoples R China. RP Xu, MG (reprint author), Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Crop Nutr & Fertilizat, Minist Agr China, Beijing 100081, Peoples R China. EM mgxu@caas.ac.cn FU Natural Science Foundation of China [41001175]; National Basic Research Program of China [2011CB100501] FX We acknowledge all our colleagues for their unremitting efforts on the long-term experiments from these sites. We thank Prof. Warren A. Dick from the Ohio State University, USA both for his critical review of the contents and for his corrections to the English in this paper. Financial supports are from the Natural Science Foundation of China (41001175) and National Basic Research Program of China (2011CB100501). NR 28 TC 17 Z9 24 U1 13 U2 87 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD FEB 15 PY 2014 VL 157 BP 47 EP 56 DI 10.1016/j.fcr.2013.12.012 PG 10 WC Agronomy SC Agriculture GA AB0TC UT WOS:000331504000006 ER PT J AU Wu, W Clark, JS Vose, JM AF Wu, Wei Clark, James S. Vose, James M. TI Response of hydrology to climate change in the southern Appalachian Mountains using Bayesian inference SO HYDROLOGICAL PROCESSES LA English DT Article DE hierarchical Bayesian models; hydrological models; climate change; streamflow; soil moisture ID UNITED-STATES; WATER-RESOURCES; PARAMETER UNCERTAINTY; METROPOLIS ALGORITHM; SOIL-MOISTURE; RUNOFF; MODELS; IMPACTS; CYCLE; SIMULATION AB Predicting long-term consequences of climate change on hydrologic processes has been limited due to the needs to accommodate the uncertainties in hydrological measurements for calibration, and to account for the uncertainties in the models that would ingest those calibrations and uncertainties in climate predictions as basis for hydrological predictions. We implemented a hierarchical Bayesian (HB) analysis to coherently admit multiple data sources and uncertainties including data inputs, parameters, and model structures to identify the potential consequences of climate change on soil moisture and streamflow at the head watersheds ranging from low to high elevations in the southern Appalachian region of the United States. We have considered climate change scenarios based on three greenhouse gas emission scenarios of the Interovernmental Panel on Climate Change: A2, A1B, and B1 emission scenarios. Full predictive distributions based on HB models are capable of providing rich information and facilitating the summarization of prediction uncertainties. With predictive uncertainties taken into account, the most pronounced change in soil moisture and streamflow would occur under the A2 scenario at both low and high elevations, followed by the A1B scenario and then by the B1 scenario. Uncertainty in the change of soil moisture is less than that of streamflow for each season, especially at high elevations. A reduction of soil moisture in summer and fall, a reduction or slight increase of streamflow in summer, and an increase of streamflow in winter are predicted for all three scenarios at both low and high elevations. The hydrological predictions with quantified uncertainties from a HB model could aid more-informed water resource management in developing mitigation plans and dealing with water security under climate change. Copyright (c) 2012 John Wiley & Sons, Ltd. C1 [Wu, Wei; Clark, James S.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Vose, James M.] US Forest Serv, USDA, Coweeta Hydrol Lab, Otto, NC 28763 USA. RP Wu, W (reprint author), Univ So Mississippi, Dept Coastal Sci, 703 East Beach Dr, Ocean Springs, MS 39564 USA. EM wei.wu@usm.edu FU National Science Foundation through Coweeta Long Term Ecological Research (LTER) FX This research was made possible by grants from the National Science Foundation through Coweeta Long Term Ecological Research (LTER). We thank USDA-Forest Service Coweeta Hydrologic Laboratory for the support of the data. We especially thank Drs. Chelcy Ford and Katherine Elliott at Coweeta Hydrological Laboratory for the insightful discussion on hydrological cycles at Coweeta basin. We also want to thank the editor and two anonymous reviewers for their helpful comments. NR 64 TC 1 Z9 1 U1 3 U2 39 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD FEB 15 PY 2014 VL 28 IS 4 BP 1616 EP 1626 DI 10.1002/hyp.9677 PG 11 WC Water Resources SC Water Resources GA 304JN UT WOS:000330743000005 ER PT J AU Martinez, G Pachepsky, YA Vereecken, H AF Martinez, Gonzalo Pachepsky, Yakov A. Vereecken, Harry TI Temporal stability of soil water content as affected by climate and soil hydraulic properties: a simulation study SO HYDROLOGICAL PROCESSES LA English DT Article DE temporal stability; soil water content; simulations; climate; water retention; saturated hydrauic conductivity ID SPATIAL-PATTERNS; PEDOTRANSFER FUNCTIONS; AGRICULTURAL FIELDS; MOISTURE; VARIABILITY; PERSISTENCE; STORAGE; TIME; DEPENDENCE; CATCHMENT AB Temporal stability of soil water content (TS SWC) is an often-observed phenomenon, which characterization finds multiple applications. Climate and variability in soil properties are usually mentioned as factors of TS SWC, but their effects are far from clear. The objective of this work was to use SWC modeling to evaluate the effects of climate and soil hydraulic properties on the TS of soil water at different measurement schedules. We selected four representative climates found in USA and simulated the multiyear SWC dynamics for sandy loam, loam, and silty clay loam soils, all having the lognormal spatial distribution of the saturated hydraulic conductivity. The CLIMGEN and the HYDRUS6 codes were used to generate weather patterns and to simulate SWC, respectively. Four different methods were applied to select the representative location (RL). The low probability of having the same variability of mean relative differences of soil water under different climates was found in most of the cases. The probability that the variance of mean relative differences depended on sampling frequency was generally higher than 91% for the three soils. The interannual difference in mean relative differences variation from short and intensive summer campaigns was highly probable for all climates and soils. The RLs changed as climate and measurement scheduling changed, and they were less pronounced for coarse-textured soils. The RL selection methods based solely on bias provided more consistency as compared with other methods. The TS appears to be the result of the interplay between climate, soil properties, and survey protocols. One implication of this factor interaction effect on TS SWC is that a simulation study can be useful to decide on the feasibility of including a search for TS-based RLs for a specific site. Copyright (c) 2013 John Wiley & Sons, Ltd. C1 [Martinez, Gonzalo] Univ Cordoba, Dept Agron, E-14071 Cordoba, Spain. [Martinez, Gonzalo; Pachepsky, Yakov A.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Vereecken, Harry] Forschungszentrum Julich, Agrosphere IBG 3, Inst Bio & Geosci, D-52428 Julich, Germany. RP Martinez, G (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. EM z42magag@uco.es RI Martinez Garcia, Gonzalo/E-3069-2013; OI Martinez Garcia, Gonzalo/0000-0001-5575-9397; Pachepsky, Yakov/0000-0003-0232-6090 FU US Department of Agriculture; US Nuclear Regulatory Commission Interagency Agreement [IAA-NRC-05-005]; Spanish Ministry of Education [EX2009-0433] FX This study was partially supported by the US Department of Agriculture and US Nuclear Regulatory Commission Interagency Agreement IAA-NRC-05-005 on "Model Abstraction Techniques to Simulate Transport in Soils'. The first author wishes to thank the Spanish Ministry of Education for the mobility grant EX2009-0433. We thank reviewers of this manuscript for their valuable comments and suggestions. NR 59 TC 16 Z9 16 U1 5 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD FEB 15 PY 2014 VL 28 IS 4 BP 1899 EP 1915 DI 10.1002/hyp.9737 PG 17 WC Water Resources SC Water Resources GA 304JN UT WOS:000330743000028 ER PT J AU Novick, K Brantley, S Miniat, CF Walker, J Vose, JM AF Novick, K. Brantley, S. Miniat, C. Ford Walker, J. Vose, J. M. TI Inferring the contribution of advection to total ecosystem scalar fluxes over a tall forest in complex terrain SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Eddy covariance; Complex terrain; Advection; Sap flux; Carbon flux; Energy balance ID ENERGY-BALANCE CLOSURE; EDDY COVARIANCE MEASUREMENTS; FREQUENCY-RESPONSE CORRECTIONS; VAPOR-PRESSURE DEFICIT; CARBON-DIOXIDE; TURBULENT FLUX; ALPINE FOREST; CO2 FLUX; EXCHANGE; RESPIRATION AB Multiple data streams from a new flux tower located in complex and heterogeneous terrain at the Coweeta Hydrologic Laboratory (North Carolina, USA) were integrated to identify periods of advective flow regimes. Drainage flows were expected a priori, due to the location of the measurement site at the base of a long, gently-sloping valley. Drainage flow was confirmed by examining vertical profile measurements of wind direction and by estimating vertical advection fluxes. The vertical advection flux of CO2 was most significant in early morning (000-0600 h) during the growing season, when it averaged similar to 5 mu mol m(-2) s(-1). Horizontal advection flux of CO2 was not directly measured in this study; however, an expected exponential relationship between nocturnal ecosystem respiration (RE) and air temperature was recovered when horizontal advection of CO2 was assumed to be negatively correlated to vertical advection, or when data were limited to periods when measured vertical advection fluxes were small. Taken together, these data imply the presence of a negative horizontal advection CO2 flux during nocturnal periods characterized by positive vertical advection of CO2. Daytime periods were characterized by consistent anabatic (up-valley) flows in mid- to late-morning (0500-1200 h) and consistent katabatic (down-valley) flows in the afternoon. A combination of above-canopy flux profile measurements, energy balance closure estimates, and flux footprint estimates suggest that during periods of up-valley wind flow, the flux footprint frequently exceeds the ecosystem dimensions, and horizontal advection fluxes related to landscape heterogeneity were a significant component of the total ecosystem flux of CO2. We used sap flux from individual trees beneath the tower to explore diurnal patterns in stomatal conductance in order to evaluate gapfilling approaches for the unreliable morning data. The relationship between stomatal conductance and vapor pressure deficit was similar in morning and afternoon periods, and we conclude that gapfilling morning data with models driven by afternoon data is a reasonable approach at this site. In general, results were consistent with other studies showing that the advection and wind flow regimes in complex terrain are highly site specific; nonetheless, the site characterization strategy developed here, when used together with independent estimates of components of the ecosystem carbon flux, could be generally applied in other sites to better understand the contribution of advection to the total ecosystem flux. (C) 2013 Elsevier B.V. All rights reserved. C1 [Novick, K.] Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN 47405 USA. [Brantley, S.; Miniat, C. Ford] USDA Forest Serv, Southern Res Stn, Coweeta Hydrol Lab, Otto, NC USA. [Brantley, S.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Walker, J.] US EPA, Natl Risk Management Res Lab, Res Triangle Pk, NC USA. [Vose, J. M.] N Carolina State Univ, USDA Forest Serv, Ctr Integrated Forest Sci, Dept Forestry & Environm Resources,Southern Res S, Raleigh, NC 27695 USA. RP Novick, K (reprint author), Indiana Univ, Sch Publ & Environm Affairs, 1315 E 10th St, Bloomington, IN 47405 USA. EM knovick@indiana.edu RI Walker, John/I-8880-2014; OI Walker, John/0000-0001-6034-7514; Miniat, Chelcy/0000-0002-3266-9783 NR 67 TC 15 Z9 15 U1 4 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD FEB 15 PY 2014 VL 185 BP 1 EP 13 DI 10.1016/j.agrformet.2013.10.010 PG 13 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 300XV UT WOS:000330498200001 ER PT J AU Grace, MH Yousef, GG Gustafson, SJ Truong, VD Yencho, GC Lila, MA AF Grace, Mary H. Yousef, Gad G. Gustafson, Sally J. Truong, Van-Den Yencho, G. Craig Lila, Mary Ann TI Phytochemical changes in phenolics, anthocyanins, ascorbic acid, and carotenoids associated with sweetpotato storage and impacts on bioactive properties SO FOOD CHEMISTRY LA English DT Article DE Sweetpotato; Phenolics; Carotenoids; Antioxidant activity; Storage; Postharvest ID IPOMOEA-BATATAS L.; ANTIOXIDANTS; ROOTS; FRUIT AB Sweetpotato phytochemical content was evaluated in four genotypes (NCPUR06-020, Covington, Yellow Covington, and NC07-847) at harvest and after curing/storage for 4 or 8 months. Curing and storage for up to 8 months did not significantly affect total phenolic content in Covington, Yellow Covington, and NC07-847, however for NCPUR06-020, a purple-fleshed selection, total phenolic content declined mainly due to anthocyanin degradation during storage. Covington had the highest carotenoid content at harvest time (281.9 mu g/g DM), followed by NC07-847 (26.2 mu g/g DM), and after 8 months, total carotenoids had increased by 25% and 50%, respectively. Antioxidant activity gradually declined during storage, and freshly harvested sweetpotatoes also demonstrated higher anti-inflammatory capacity as gauged by inhibition of lipopolysaccharide-induced reactive oxygen species (ROS) in SH-SY5Y cells. Gradual changes in sweetpotato phytochemical content and antioxidant and anti-inflammatory capacity were noted during normal long-term storage, but the specific effects were genotype-dependent. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Grace, Mary H.; Yousef, Gad G.; Gustafson, Sally J.; Lila, Mary Ann] N Carolina State Univ, Plants Human Hlth Inst, Dept Food Bioproc & Nutr Sci, Kannapolis, NC 28081 USA. [Grace, Mary H.; Yousef, Gad G.; Truong, Van-Den; Lila, Mary Ann] N Carolina State Univ, USDA ARS, Dept Food Bioproc & Nutr Sci, SAA Food Sci Res Unit, Raleigh, NC 27695 USA. [Yousef, Gad G.; Yencho, G. Craig] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. RP Lila, MA (reprint author), N Carolina State Univ, Plants Human Hlth Inst, Dept Food Bioproc & Nutr Sci, 600 Laureate Way, Kannapolis, NC 28081 USA. EM maryann_lila@ncsu.edu OI Grace, Mary/0000-0002-8536-4211 FU North Carolina SweetPotato Commission FX We gratefully acknowledge the financial support of the North Carolina SweetPotato Commission. NR 31 TC 20 Z9 20 U1 5 U2 66 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD FEB 15 PY 2014 VL 145 BP 717 EP 724 DI 10.1016/j.foodchem.2013.08.107 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 261SP UT WOS:000327685200097 PM 24128536 ER PT J AU Cassone, BJ Wijeratne, S Michel, AP Stewart, LR Chen, YT Yan, P Redinbaugh, MG AF Cassone, Bryan J. Wijeratne, Saranga Michel, Andrew P. Stewart, Lucy R. Chen, Yuting Yan, Pearlly Redinbaugh, Margaret G. TI Virus-independent and common transcriptome responses of leafhopper vectors feeding on maize infected with semi-persistently and persistent propagatively transmitted viruses SO BMC GENOMICS LA English DT Article DE Gene expression; Leafhopper; Nucleorhabdovirus; Waikavirus; Viral transmission pathogen response; Innate; immunity ID CELLULAR IMMUNE-RESPONSE; CHLOROTIC DWARF VIRUS; HEAT-SHOCK PROTEINS; FINE STREAK-VIRUS; LARGE GENE LISTS; PLANT-VIRUSES; RNA-SEQ; DROSOPHILA-MELANOGASTER; SEMIPERSISTENT TRANSMISSION; INTRACELLULAR-TRANSPORT AB Background: Insects are the most important epidemiological factors for plant virus disease spread, with > 75% of viruses being dependent on insects for transmission to new hosts. The black-faced leafhopper (Graminella nigrifrons Forbes) transmits two viruses that use different strategies for transmission: Maize chlorotic dwarf virus (MCDV) which is semi-persistently transmitted and Maize fine streak virus (MFSV) which is persistently and propagatively transmitted. To date, little is known regarding the molecular and cellular mechanisms in insects that regulate the process and efficiency of transmission, or how these mechanisms differ based on virus transmission strategy. Results: RNA-Seq was used to examine transcript changes in leafhoppers after feeding on MCDV-infected, MFSV-infected and healthy maize for 4 h and 7 d. After sequencing cDNA libraries constructed from whole individuals using Illumina next generation sequencing, the Rnnotator pipeline in Galaxy was used to reassemble the G. nigrifrons transcriptome. Using differential expression analyses, we identified significant changes in transcript abundance in G. nigrifrons. In particular, transcripts implicated in the innate immune response and energy production were more highly expressed in insects fed on virus-infected maize. Leafhoppers fed on MFSV-infected maize also showed an induction of transcripts involved in hemocoel and cell-membrane linked immune responses within four hours of feeding. Patterns of transcript expression were validated for a subset of transcripts by quantitative real-time reverse transcription polymerase chain reaction using RNA samples collected from insects fed on healthy or virus-infected maize for between a 4 h and seven week period. Conclusions: We expected, and found, changes in transcript expression in G. nigrifrons feeding of maize infected with a virus (MFSV) that also infects the leafhopper, including induction of immune responses in the hemocoel and at the cell membrane. The significant induction of the innate immune system in G. nigrifrons fed on a foregut- borne virus (MCDV) that does not infect leafhoppers was less expected. The changes in transcript accumulation that occur independent of the mode of pathogen transmission could be key for identifying insect factors that disrupt vector-mediated plant virus transmission. C1 [Cassone, Bryan J.; Stewart, Lucy R.; Redinbaugh, Margaret G.] ARS, USDA, Corn Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. [Wijeratne, Saranga] Ohio State Univ, Ohio Agr Res & Dev Ctr, Mol & Cellular Imaging Ctr, Wooster, OH 44691 USA. [Michel, Andrew P.; Chen, Yuting] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Stewart, Lucy R.; Redinbaugh, Margaret G.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA. [Yan, Pearlly] Ohio State Univ, Human Canc Genet Program, Columbus, OH 43210 USA. [Yan, Pearlly] Ohio State Univ, Sch Biomed Sci, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA. RP Redinbaugh, MG (reprint author), ARS, USDA, Corn Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. EM peg.redinbaugh@ars.usda.gov RI Redinbaugh, Margaret/A-3611-2013; Yan, Pearlly/E-4339-2011; Wijeratne, Saranga/I-5341-2016 OI Yan, Pearlly/0000-0003-1965-4920; Wijeratne, Saranga/0000-0002-9782-4406 NR 66 TC 9 Z9 9 U1 0 U2 18 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 14 PY 2014 VL 15 AR 133 DI 10.1186/1471-2164-15-133 PG 13 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AC5ZT UT WOS:000332600300002 PM 24524215 ER PT J AU Chang, XX Donnelly, L Sun, DY Rao, JP Reid, MS Jiang, CZ AF Chang, Xiaoxiao Donnelly, Linda Sun, Daoyang Rao, Jingping Reid, Michael S. Jiang, Cai-Zhong TI A Petunia Homeodomain-Leucine Zipper Protein, PhHD-Zip, Plays an Important Role in Flower Senescence SO PLOS ONE LA English DT Article ID ABSCISIC-ACID; ARABIDOPSIS-THALIANA; WATER-DEFICIT; ETHYLENE BIOSYNTHESIS; SENESCING PETALS; ENDOGENOUS ABA; SHOOT GROWTH; DNA-BINDING; GENES; TRANSCRIPTION AB Flower senescence is initiated by developmental and environmental signals, and regulated by gene transcription. A homeodomain-leucine zipper transcription factor, PhHD-Zip, is up-regulated during petunia flower senescence. Virus-induced gene silencing of PhHD-Zip extended flower life by 20% both in unpollinated and pollinated flowers. Silencing PhHD-Zip also dramatically reduced ethylene production and the abundance of transcripts of genes involved in ethylene (ACS, ACO), and ABA (NCED) biosynthesis. Abundance of transcripts of senescence-related genes (SAG12, SAG29) was also dramatically reduced in the silenced flowers. Over-expression of PhHD-Zip accelerated petunia flower senescence. Furthermore, PhHD-Zip transcript abundance in petunia flowers was increased by application of hormones (ethylene, ABA) and abiotic stresses (dehydration, NaCl and cold). Our results suggest that PhHD-Zip plays an important role in regulating petunia flower senescence. C1 [Chang, Xiaoxiao; Sun, Daoyang; Rao, Jingping] Northwest A&F Univ, Dept Hort, Yangling, Shaanxi, Peoples R China. [Chang, Xiaoxiao; Sun, Daoyang; Reid, Michael S.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Donnelly, Linda; Jiang, Cai-Zhong] USDA, Crops Pathol & Genet Res Unit, ARS, Davis, CA USA. RP Rao, JP (reprint author), Northwest A&F Univ, Dept Hort, Yangling, Shaanxi, Peoples R China. EM dqr0723@163.com; msreid@ucdavis.edu; cjiang@ucdavis.edu FU United States Department of Agriculture (USDA) CRIS [5306-21000-019-00D]; USDA Floriculture Initiative [5306-13210-001-02S]; National Key Technology Research and Development Program of the Ministry of Science and Technology of China [2013BAD19B04] FX This work was partially supported by United States Department of Agriculture (USDA) CRIS project 5306-21000-019-00D, USDA Floriculture Initiative (5306-13210-001-02S) and National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2013BAD19B04). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 10 Z9 11 U1 4 U2 31 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 14 PY 2014 VL 9 IS 2 AR e88320 DI 10.1371/journal.pone.0088320 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AA7IR UT WOS:000331271500035 PM 24551088 ER PT J AU Paul, G Gowda, PH Prasad, PVV Howell, TA Aiken, RM Neale, CMU AF Paul, George Gowda, Prasanna H. Prasad, P. V. Vara Howell, Terry A. Aiken, Robert M. Neale, Christopher M. U. TI Investigating the influence of roughness length for heat transport (z(oh)) on the performance of SEBAL in semi-arid irrigated and dryland agricultural systems SO JOURNAL OF HYDROLOGY LA English DT Article DE SEBAL; Excess resistance kB(-1); Lysimeter; Airborne Remote Sensing; Aerodynamic temperature; Roughness length sensitivity ID SURFACE-ENERGY BALANCE; SENSING-BASED MODELS; REMOTELY-SENSED DATA; VEGETATED SURFACES; EVAPOTRANSPIRATION ESTIMATION; MAPPING EVAPOTRANSPIRATION; TEMPERATURE RELATIONSHIP; FIELD-MEASUREMENTS; SPARSE VEGETATION; WATER-RESOURCES AB Satellite-based thermal infrared remote sensing has greatly contributed to the development and improvement of remote sensing-based evapotranspiration (RS-ET) mapping algorithms. Radiometric temperature (T-s) derived from thermal sensors is inherently different from the aerodynamic temperature (T-o) required for solving the bulk formulation of sensible heat (H). The scalar roughness length (z(oh)) representing heat transport mechanism and described by the dimensionless parameter kB(-1) was used to account for the discrepancy between T-s and T-o. Surface Energy Balance Algorithm for Land (SEBAL), with its indigenous approach of linearly relating dT (near-surface temperature gradient) with T-s across the imagery, maintained that this approach would absorb the impacts of differences between T-s and T-o. Therefore, it utilized a constant kB(-1) value of 2.3 in its initial version, and later switched to a constant z(oh) (z(1)) value of 0.1. In this study, we investigated the influence of these changes in SEBAL by testing four different approaches: (i) z(oh) derived from a constant kB(-1) of 2.3, (ii) constant z(oh) (z(1)) = 0.1 m, (iii) constant z(oh) (z(1)) = 0.01 m, and (iv) spatially variable z(oh) from kB(-1) parameterization. SEBAL was applied on 10 high-resolution airborne images acquired during BEAREX07-08 (Bushland Evapotranspiration and Agricultural Remote Sensing Experiment) and validated against measurements from four large weighing lysimeters installed on two irrigated and two dryland fields. The spatially variable kB(-1) produced statistically different and improved ET estimates compared to that with constant kB(-1) and constant z(1) (z(oh)) approaches. SEBAL performance for irrigated fields representing high ET and complete ground cover surfaces was markedly different from that for dryland fields representing greater soil water deficits with sparser vegetation cover. A variable kB(-1) value derived from a physical model generated good overall estimates while delivering improved performance for dryland agricultural systems. Overall, this study focused on the classical problem of estimating heat transfer from two contrasting hydrological regimes i.e. irrigated and dryland agriculture and illustrated the existing need for a realistic consideration of excess resistance to heat transfer in single-source resistance modeling frameworks. (C) 2013 Elsevier B.V. All rights reserved. C1 [Paul, George; Prasad, P. V. Vara; Aiken, Robert M.] Kansas State Univ, Manhattan, KS 66506 USA. [Gowda, Prasanna H.; Howell, Terry A.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Neale, Christopher M. U.] Utah State Univ, Logan, UT 84322 USA. RP Paul, G (reprint author), Kansas State Univ, 2004 Throckmorton Hall, Manhattan, KS 66506 USA. EM gpaul@ksu.edu RI Prasad, P.V. Vara/B-3835-2012; Neale, Christopher/P-3676-2015; OI Prasad, P.V. Vara/0000-0001-6632-3361; Neale, Christopher/0000-0002-7199-6410; Paul, George/0000-0002-5322-7650 FU USDA-Agricultural Research Service; Kansas State University; Texas A&M AgriLife Research; Texas A&M AgriLife Extension Service; Texas Tech University; West Texas AM University FX This research was supported by the Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. This is contribution number 13-064-J from the Kansas Agricultural Experiment Station. NR 77 TC 8 Z9 8 U1 5 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD FEB 13 PY 2014 VL 509 BP 231 EP 244 DI 10.1016/j.jhydrol.2013.11.040 PG 14 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA AB3AD UT WOS:000331662800019 ER PT J AU Wang, L Wu, JQ Elliot, WJ Fiedler, FR Lapin, S AF Wang, Li Wu, Joan Q. Elliot, William J. Fiedler, Fritz R. Lapin, Sergey TI Linear diffusion-wave channel routing using a discrete Hayami convolution method SO JOURNAL OF HYDROLOGY LA English DT Article DE Linear diffusion-wave channel routing; Discrete Hayami convolution; Kernel function values; Mass-balance error; Temporal resolution; Lateral inflow ID MIXING-CELL METHOD; CASCADE MODEL; LATERAL INFLOW; BANK STORAGE; STREAM-STAGE; FLOOD; EQUATION AB The convolution of an input with a response function has been widely used in hydrology as a means to solve various problems analytically. Due to the high computation demand in solving the functions using numerical integration, it is often advantageous to use the discrete convolution instead of the integration of the continuous functions. This approach greatly reduces the amount of the computational work; however, it increases the possibility for mass balance errors. In this study, we analyzed the characteristics of the kernel function for the Hayami convolution solution to the linear diffusion-wave channel routing with distributed lateral inflow. We propose two ways of selection of the discrete kernel function values: using the exact point values or using the center-averaged values. Through a hypothetical example and the applications to Asotin Creek, WA and the Clearwater River, ID, we showed that when the point kernel function values were used in the discrete Hayami convolution (DHC) solution, the mass balance error of channel routing is dependent on the number of time steps on the rising limb of the Hayami kernel function. The mass balance error is negligible when there are more than 1.8 time steps on the rising limb of the kernel function. The fewer time steps on the rising limb, the greater risk of high mass balance errors. When the average kernel function values are used for the DHC solution, however, the mass balance is always maintained, since the integration of the discrete kernel function is always unity. (C) 2013 Elsevier B.V. All rights reserved. C1 [Wang, Li; Wu, Joan Q.] Washington State Univ, Dept Biol Syst Engn, Puyallup Res & Extens Ctr, Puyallup, WA 98371 USA. [Elliot, William J.] Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Fiedler, Fritz R.] Univ Idaho, Dept Civil Engn, Moscow, ID 83844 USA. [Lapin, Sergey] Washington State Univ, Dept Math, Pullman, WA 99164 USA. RP Wang, L (reprint author), GeoEngineers Inc, Tacoma, WA 98402 USA. EM liwang@wsu.edu RI Lapin, Sergey/E-4237-2015 FU USDA CSREES CEAP Grant [2008-48686-04903]; Washington State University FX This work was supported by the USDA CSREES CEAP Grant (No. 2008-48686-04903) and Washington State University. We are grateful to the two anonymous reviewers, Associate Editor, and Editor-in-Chief for their valuable comments and suggestions that have helped to improve the rigor and clarity of this manuscript. NR 36 TC 2 Z9 2 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD FEB 13 PY 2014 VL 509 BP 282 EP 294 DI 10.1016/j.jhydrol.2013.11.046 PG 13 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA AB3AD UT WOS:000331662800023 ER PT J AU Templeton, RC Vivoni, ER Mendez-Barroso, LA Pierini, NA Anderson, CA Rango, A Laliberte, AS Scott, RL AF Templeton, Ryan C. Vivoni, Enrique R. Mendez-Barroso, Luis A. Pierini, Nicole A. Anderson, Cody A. Rango, Albert Laliberte, Andrea S. Scott, Russell L. TI High-resolution characterization of a semiarid watershed: Implications on evapotranspiration estimates SO JOURNAL OF HYDROLOGY LA English DT Article DE Watershed hydrology; Mixed shrubland; Environmental sensor network; Soil moisture; Evapotranspiration; Unmanned Aerial Vehicle ID SPATIAL VARIABILITY; NORTHWEST MEXICO; COMPLEX TERRAIN; SOIL-MOISTURE; WOODY-PLANTS; MONSOON; CATCHMENT; GRASSLAND; DYNAMICS; RAINFALL AB The North American monsoon (NAM) contributes roughly half of the annual precipitation in the Chihuahuan Desert from July to September. Relatively frequent, intense storms increase soil moisture and lead to ephemeral runoff. Quantifying these processes, however, is difficult due to the sparse nature of existing observations. This study presents results from a dense network of rain gauges, soil probes, channel flumes, and an eddy covariance tower in a small watershed of the Jornada Experimental Range. Using this network, the temporal and spatial variability of soil moisture conditions and channel runoff were assessed from June 2010 to September 2011. In addition, tower measurements were used to quantify the seasonal, monthly and event-scale changes in land-atmosphere states and fluxes. Results from this study indicate a strong seasonality in water and energy fluxes, with a reduction in the Bowen ratio (B) from winter (B = 14) to summer (B = 3.3). This reduction was tied to higher shallow soil moisture (theta) availability during the summer (0 = 0.040 m(3)/m(3)) as compared to winter (0 = 0.004 m(3)/m(3)). Four consecutive rainfall-runoff events during the NAM were used to quantify the soil moisture and channel runoff responses and how water availability impacted land-atmosphere fluxes. The network also allowed comparisons of several approaches to estimate evapotranspiration (ET). using a water balance residual approach, a more accurate ET estimate was obtained when distributed measurements were used, as opposed to single site measurements at the tower. In addition, the spatially-varied soil moisture data yielded a more reasonable daily relation between ET and 0, an important parameterization in many hydrologic models. These analyses illustrate the value of high-resolution sampling in small watersheds to characterize hydrologic processes. (C) 2013 Elsevier B.V. All rights reserved. C1 [Templeton, Ryan C.; Vivoni, Enrique R.; Pierini, Nicole A.; Anderson, Cody A.] Arizona State Univ, Sch Sustainable Engn & Built Environm, Tempe, AZ 85287 USA. [Vivoni, Enrique R.; Mendez-Barroso, Luis A.] Arizona State Univ, Sch Earth & Space Explorat, Tempe, AZ 85287 USA. [Rango, Albert] USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. [Laliberte, Andrea S.] Earthmetrics, Brownsville, OR 97327 USA. [Scott, Russell L.] USDA ARS Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. RP Vivoni, ER (reprint author), Arizona State Univ, Sch Earth & Space Explorat, ISTB4,Bldg 75,Room 769, Tempe, AZ 85287 USA. EM vivoni@asu.edu RI Vivoni, Enrique/E-1202-2012; Mendez-Barroso, Luis/D-2884-2015 OI Vivoni, Enrique/0000-0002-2659-9459; FU U.S. Army Research Office [56059-EV-PCS]; Jornada Long-Term Ecological Research project (National Science Foundation) [DEB-1235828] FX We thank Craig Tweedie, Aline Jaimes amd Gesuri Ramirez from the University of Texas at El Paso for help with implementing a telemetry network at the site. We are also grateful for help from John Anderson and staff members at the Jornada Experimental Range. We thank funding from the U.S. Army Research Office (Grant 56059-EV-PCS) and the Jornada Long-Term Ecological Research project (National Science Foundation Grant DEB-1235828). We acknowledge two reviewers whose comments helped improve the original manuscript. NR 76 TC 11 Z9 12 U1 2 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD FEB 13 PY 2014 VL 509 BP 306 EP 319 DI 10.1016/j.jhydrol.2013.11.047 PG 14 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA AB3AD UT WOS:000331662800025 ER PT J AU Chen, HD Seiber, JN Hotze, M AF Chen, Hongda Seiber, James N. Hotze, Matt TI ACS Select on Nanotechnology in Food and Agriculture: A Perspective on Implications and Applications SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Editorial Material ID NANOPARTICLES; PRODUCTS; SOIL C1 [Chen, Hongda] USDA, Natl Inst Food & Agr, Washington, DC 20250 USA. [Seiber, James N.] Univ Calif Davis, Dept Environm Toxicol, Davis, CA 95616 USA. [Hotze, Matt] Amer Chem Soc, Journal Agr & Food Chem, Washington, DC 20036 USA. RP Chen, HD (reprint author), USDA, Natl Inst Food & Agr, 1400 Independence Ave SW, Washington, DC 20250 USA. EM hchen@nifa.usda.gov NR 20 TC 17 Z9 18 U1 1 U2 55 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 12 PY 2014 VL 62 IS 6 BP 1209 EP 1212 DI 10.1021/jf5002588 PG 4 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AA8KF UT WOS:000331343700001 PM 24479582 ER PT J AU Vensel, WH Tanaka, CK Altenbach, SB AF Vensel, William H. Tanaka, Charlene K. Altenbach, Susan B. TI Protein composition of wheat gluten polymer fractions determined by quantitative two-dimensional gel electrophoresis and tandem mass spectrometry SO PROTEOME SCIENCE LA English DT Article DE Chain-terminating gliadins; Gluten polymer; Size-exclusion chromatography; Wheat flour quality ID PERFORMANCE LIQUID-CHROMATOGRAPHY; SODIUM DODECYL-SULFATE; FLOUR PROTEINS; GRAIN DEVELOPMENT; STORAGE PROTEINS; HIGH-TEMPERATURE; SUBUNITS; ACCUMULATION; FERTILIZER; ENDOSPERM AB Background: Certain wheat gluten proteins form large protein polymers that are extractable in 0.5% SDS only after sonication. Although there is a strong relationship between the amounts of these polymers in the flour and bread-making quality, the protein components of these polymers have not been thoroughly investigated. Results: Flour proteins from the US bread wheat Butte 86 were extracted in 0.5% SDS using a two-step procedure with and without sonication. Proteins were further separated by size exclusion chromatography (SEC) into monomeric and polymeric fractions and analyzed by quantitative two-dimensional gel electrophoresis (2-DE). When proteins in select 2-DE spots were identified by tandem mass spectrometry (MS/MS), overlapping spots from the different protein fractions often yielded different identifications. Most high-molecular-weight glutenin subunits (HMW-GS) and low-molecular-weight glutenin subunits (LMW-GS) partitioned into the polymer fractions, while most gliadins were found in the monomer fractions. The exceptions were alpha, gamma and omega gliadins containing odd numbers of cysteine residues. These proteins were detected in all fractions, but comprised the largest proportion of the SDS-extractable polymer fraction. Several types of non-gluten proteins also were found in the polymer fractions, including serpins, triticins and globulins. All three types were found in the largest proportions in the SDS-extractable polymer fraction. Conclusions: This is the first study to report the accumulation of gliadins containing odd numbers of cysteine residues in the SDS-extractable glutenin polymer fraction, supporting the hypothesis that these gliadins serve as chain terminators of the polymer chains. These data make it possible to formulate hypotheses about how protein composition influences polymer size and structure and provide a foundation for future experiments aimed at determining how environment affects glutenin polymer distribution. In addition, the analysis revealed additional layers of complexity to the wheat flour proteome that should be considered when evaluating quantitative 2-DE data. C1 [Vensel, William H.; Tanaka, Charlene K.; Altenbach, Susan B.] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Vensel, WH (reprint author), ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM william.vensel@ars.usda.gov FU USDA Agricultural Research Service CRIS Project [5325-43000-027-00D] FX The authors thank Dr. Donald Kasarda for continuous discussion of the many aspects of the glutenin polymer and for review of the manuscript and Les Harden for setup of the mass spectrometry instrumentation and determination of the best instrument parameters. This research was funded by USDA Agricultural Research Service CRIS Project 5325-43000-027-00D. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. NR 31 TC 5 Z9 6 U1 6 U2 47 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1477-5956 J9 PROTEOME SCI JI Proteome Sci. PD FEB 11 PY 2014 VL 12 AR 8 DI 10.1186/1477-5956-12-8 PG 13 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA AC8RA UT WOS:000332800100001 PM 24517725 ER PT J AU Bearson, BL Allen, HK Brunelle, BW Lee, IS Casjene, SR Stanton, TB AF Bearson, Bradley L. Allen, Heather K. Brunelle, Brian W. Lee, In Soo Casjene, Sherwood R. Stanton, Thaddeus B. TI The agricultural antibiotic carbadox induces phage-mediated gene transfer in Salmonella SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE Salmonella; bacteriophage; antibiotic; carbadox; prophage; gene transfer; transduction ID ENTERICA SEROVAR TYPHIMURIUM; COMPLETE NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI STRAINS; LYSOGENIC CONVERSION; RESISTANCE; BACTERIOPHAGE; PROPHAGE; VIRULENCE; GROWTH; TOXIN AB Antibiotics are used for disease therapeutic or preventative effects in humans and animals, as well as for enhanced feed conversion efficiency in livestock. Antibiotics can also cause undesirable effects in microbial populations, including selection for antibiotic resistance, enhanced pathogen invasion, and stimulation of horizontal gene transfer. Carbadox is a veterinary antibiotic used in the US during the starter phase of swine production for improved feed efficiency and control of swine dysentery and bacterial swine enteritis. Carbadox has been shown in vitro to induce phage-encoded Shiga toxin in Shiga toxin-producing Escherichia coli (STEC) and a phage-like element transferring antibiotic resistance genes in Brachyspira hyodysenteriae, but the effect of carbadox on prophages in other bacteria is unknown. This study examined carbadox exposure on prophage induction and genetic transfer in Salmonella enterica serovar Typhimurium, a human foodborne pathogen that frequently colonizes swine without causing disease. S. Typhimurium LT2 exposed to carbadox induced prophage production, resulting in bacterial cell lysis and release of virions that were visible by electron microscopy. Carbadox induction of phage-mediated gene transfer was confirmed by monitoring the transduction of a sodCIII::neo cassette in the Fels-1 prophage from LT2 to a recipient Salmonella strain. Furthermore, carbadox frequently induced generalized transducing phages in multidrug-resistant phage type DT104 and DT120 isolates, resulting in the transfer of chromosomal and plasmid DNA that included antibiotic resistance genes. Our research indicates that exposure of Salmonella to carbadox induces prophages that can transfer virulence and antibiotic resistance genes to susceptible bacterial hosts. Carbadox-induced, phage-mediated gene transfer could serve as a contributing factor in bacterial evolution during animal production, with prophages being a reservoir for bacterial fitness genes in the environment. C1 [Bearson, Bradley L.; Lee, In Soo] USDA ARS, Agroecosyst Management Res Unit, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Allen, Heather K.; Brunelle, Brian W.; Stanton, Thaddeus B.] USDA ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Ames, IA 50011 USA. [Lee, In Soo] Hannam Univ, Dept Biol Sci & Biotechnol, Taejon, South Korea. [Casjene, Sherwood R.] Univ Utah, Dept Pathol, Div Microbiol & Immunol, Salt Lake City, UT USA. RP Bearson, BL (reprint author), USDA ARS, Agroecosyst Management Res Unit, Natl Lab Agr & Environm, 2110 Univ Dr,NSRIC-2103, Ames, IA 50011 USA. EM brad.bearson@ars.usda.gov NR 57 TC 16 Z9 16 U1 1 U2 18 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 11 PY 2014 VL 5 AR 52 DI 10.3389/fmicb.2014.00052 PG 8 WC Microbiology SC Microbiology GA AB4WL UT WOS:000331790500001 PM 24575089 ER PT J AU Seguin, J Rajeswaran, R Malpica-Lopez, N Martin, RR Kasschau, K Dolja, VV Otten, P Farinelli, L Pooggin, MM AF Seguin, Jonathan Rajeswaran, Rajendran Malpica-Lopez, Nachelli Martin, Robert R. Kasschau, Kristin Dolja, Valerian V. Otten, Patricia Farinelli, Laurent Pooggin, Mikhail M. TI De Novo Reconstruction of Consensus Master Genomes of Plant RNA and DNA Viruses from siRNAs SO PLOS ONE LA English DT Article ID MOSAIC-VIRUS; SEQUENCE; GRAPEVINE; EXPRESSION; DISCOVERY; IDENTIFICATION; GEMINIVIRUSES; METHYLATION; NUCLEOTIDE; DIAGNOSIS AB Virus-infected plants accumulate abundant, 21-24 nucleotide viral siRNAs which are generated by the evolutionary conserved RNA interference (RNAi) machinery that regulates gene expression and defends against invasive nucleic acids. Here we show that, similar to RNA viruses, the entire genome sequences of DNA viruses are densely covered with siRNAs in both sense and antisense orientations. This implies pervasive transcription of both coding and non-coding viral DNA in the nucleus, which generates double-stranded RNA precursors of viral siRNAs. Consistent with our finding and hypothesis, we demonstrate that the complete genomes of DNA viruses from Caulimoviridae and Geminiviridae families can be reconstructed by deep sequencing and de novo assembly of viral siRNAs using bioinformatics tools. Furthermore, we prove that this 'siRNA omics' approach can be used for reliable identification of the consensus master genome and its microvariants in viral quasispecies. Finally, we utilized this approach to reconstruct an emerging DNA virus and two viroids associated with economically-important red blotch disease of grapevine, and to rapidly generate a biologically-active clone representing the wild type master genome of Oilseed rape mosaic virus. Our findings show that deep siRNA sequencing allows for de novo reconstruction of any DNA or RNA virus genome and its microvariants, making it suitable for universal characterization of evolving viral quasispecies as well as for studying the mechanisms of siRNA biogenesis and RNAi-based antiviral defense. C1 [Seguin, Jonathan; Rajeswaran, Rajendran; Malpica-Lopez, Nachelli; Pooggin, Mikhail M.] Univ Basel, Inst Bot, Dept Environm Sci, CH-4056 Basel, Switzerland. [Martin, Robert R.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. [Martin, Robert R.; Kasschau, Kristin; Dolja, Valerian V.] Oregon State Univ, Dept Bot & Plant Pathol, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Seguin, Jonathan; Otten, Patricia; Farinelli, Laurent] Fasteris SA, Plan Les Ouates, Geneva, Switzerland. RP Pooggin, MM (reprint author), Univ Basel, Inst Bot, Dept Environm Sci, Hebelstr 1, CH-4056 Basel, Switzerland. EM Mikhail.Pooggin@unibas.ch FU European Cooperation in Science and Technology [C09.0176]; Swiss National Science Foundation [31003A_143882/1]; Vinoculate, Inc. [2010-744]; USDA-NIFA [2009-04401]; USDA-NIFA-SCRI [2009-51181-06027]; Bard [IS-4314-10C] FX The work was supported by European Cooperation in Science and Technology [grant SERI No. C09.0176 to L.F. and M.M.P.]; Swiss National Science Foundation [grant 31003A_143882/1 to M.M.P.]; Vinoculate, Inc. [contract 2010-744 to V.V.D.], USDA-NIFA [subcontract 2009-04401 to V.V.D.]; USDA-NIFA-SCRI [2009-51181-06027 subaward to R.R.M.]; and Bard [award IS-4314-10C to V.V.D.]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 34 Z9 34 U1 2 U2 20 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 11 PY 2014 VL 9 IS 2 AR e88513 DI 10.1371/journal.pone.0088513 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AA7DT UT WOS:000331258100056 PM 24523907 ER PT J AU Malik, VS AF Malik, Vedpal Singh TI Building research centres of distinction for action SO CURRENT SCIENCE LA English DT Letter C1 USDA, Riverdale, MD 20737 USA. RP Malik, VS (reprint author), USDA, 4700 River Rd, Riverdale, MD 20737 USA. EM Vedpalm@gmail.corn NR 0 TC 0 Z9 0 U1 0 U2 0 PU INDIAN ACAD SCIENCES PI BANGALORE PA C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA SN 0011-3891 J9 CURR SCI INDIA JI Curr. Sci. PD FEB 10 PY 2014 VL 106 IS 3 BP 342 EP 343 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AD7JK UT WOS:000333439900004 ER PT J AU Sleddens, EFC O'Connor, TM Watson, KB Hughes, SO Power, TG Thijs, C De Vries, NK Kremers, SPJ AF Sleddens, Ester F. C. O'Connor, Teresia M. Watson, Kathleen B. Hughes, Sheryl O. Power, Thomas G. Thijs, Carel De Vries, Nanne K. Kremers, Stef P. J. TI Development of the Comprehensive General Parenting Questionnaire for caregivers of 5-13 year olds SO INTERNATIONAL JOURNAL OF BEHAVIORAL NUTRITION AND PHYSICAL ACTIVITY LA English DT Article DE Body mass index; Children; General parenting; Questionnaire development; Parent personality; Validation ID PSYCHOLOGICAL CONTROL; PHYSICAL-ACTIVITY; CHILDRENS REPORTS; 5-FACTOR MODEL; BEHAVIOR; PERSONALITY; WEIGHT; AUTONOMY; CONCEPTUALIZATION; INTERVENTIONS AB Background: Despite the large number of parenting questionnaires, considerable disagreement exists about how to best assess parenting. Most of the instruments only assess limited aspects of parenting. To overcome this shortcoming, the "Comprehensive General Parenting Questionnaire" (CGPQ) was systematically developed. Such a measure is frequently requested in the area of childhood overweight. Methods: First, an item bank of existing parenting measures was created assessing five key parenting constructs that have been identified across multiple theoretical approaches to parenting (Nurturance, Overprotection, Coercive control, Behavioral control, and Structure). Caregivers of 5- to 13-year-olds were asked to complete the online survey in the Netherlands (N = 821), Belgium (N = 435) and the United States (N = 241). In addition, a questionnaire regarding personality characteristics ("Big Five") of the caregiver was administered and parents were asked to report about their child's height and weight. Factor analyses and Item-Response Modeling (IRM) techniques were used to assess the underlying parenting constructs and for item reduction. Correlation analyses were performed to assess the relations between general parenting and personality of the caregivers, adjusting for socio-economic status (SES) indicators, to establish criterion validity. Multivariate linear regressions were performed to examine the associations of SES indicators and parenting with child BMI z-scores. Additionally, we assessed whether scores on the parenting constructs and child BMI z-scores differed depending on SES indicators. Results: The reduced questionnaire (62 items) revealed acceptable fit of our parenting model and acceptable IRM item fit statistics. Caregiver personality was related as hypothesized with the GCPQ parenting constructs. While correcting for SES, overprotection was positively related to child BMI. The negative relationship between structure and BMI was borderline significant. Parents with a high level of education were less likely to use overly forms of controlling parenting (i.e., coercive control and overprotection) and more likely to have children with lower BMI. Based on several author review meetings and cognitive interviews the questionnaire was further modified to an 85-item questionnaire. Conclusions: The GCPQ may facilitate research exploring how parenting influences children's weight-related behaviors. The contextual influence of general parenting is likely to be more profound than its direct relationship with weight status. C1 [Sleddens, Ester F. C.; Kremers, Stef P. J.] Maastricht Univ, Med Ctr, NUTRIM Sch Nutr Toxicol & Metab, Dept Hlth Promot, NL-6200 MD Maastricht, Netherlands. [O'Connor, Teresia M.; Watson, Kathleen B.; Hughes, Sheryl O.] USDA, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Power, Thomas G.] Washington State Univ, Dept Human Dev, Pullman, WA 99164 USA. [Thijs, Carel] Maastricht Univ, CAPHRI Sch Publ Hlth & Primary Care, Dept Epidemiol, NL-6200 MD Maastricht, Netherlands. [De Vries, Nanne K.] Maastricht Univ, Med Ctr, CAPHRI Sch Publ Hlth, Dept Hlth Promot, NL-6200 MD Maastricht, Netherlands. [De Vries, Nanne K.] Maastricht Univ, Med Ctr, NUTRIM Sch Nutr Toxicol & Metab, NL-6200 MD Maastricht, Netherlands. RP Sleddens, EFC (reprint author), Maastricht Univ, Med Ctr, NUTRIM Sch Nutr Toxicol & Metab, Dept Hlth Promot, POB 616, NL-6200 MD Maastricht, Netherlands. EM Ester.Sleddens@maastrichtuniversity.nl FU Netherlands Heart Foundation [2008B112]; Baylor College of Medicine/USDA Children's Nutrition Research Center FX This research was funded by the Netherlands Heart Foundation (project number 2008B112) and Baylor College of Medicine/USDA Children's Nutrition Research Center internal funds. NR 85 TC 11 Z9 11 U1 3 U2 18 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1479-5868 J9 INT J BEHAV NUTR PHY JI Int. J. Behav. Nutr. Phys. Act. PD FEB 10 PY 2014 VL 11 AR 15 DI 10.1186/1479-5868-11-15 PG 14 WC Nutrition & Dietetics; Physiology SC Nutrition & Dietetics; Physiology GA AD0OE UT WOS:000332932900002 PM 24512450 ER PT J AU Powell, JA Bentz, BJ AF Powell, James A. Bentz, Barbara J. TI Phenology and density-dependent dispersal predict patterns of mountain pine beetle (Dendroctonus ponderosae) impact SO ECOLOGICAL MODELLING LA English DT Article DE Bark beetle; Motility; Temperature; Phenology; Ecological diffusion; Outbreak pattern ID WESTERN UNITED-STATES; BRITISH-COLUMBIA; RANGE EXPANSION; BARK BEETLES; SPATIOTEMPORAL PATTERNS; COLEOPTERA-SCOLYTIDAE; SPATIAL SYNCHRONY; OUTBREAK POPULATIONS; MODEL ANALYSIS; CLIMATE-CHANGE AB For species with irruptive population behavior, dispersal is an important component of outbreak dynamics. We developed and parameterized a mechanistic model describing mountain pine beetle (Dendroctonus ponderosae Hopkins) population demographics and dispersal across a landscape. Model components include temperature-dependent phenology, host tree colonization determined by an Allee effect, and random-walk dispersal with motility conditioned by host tree density. The model was parameterized at a study site in central Idaho, United States (US), and evaluated at an independent site in northern Washington, US. Phloem and air temperatures, MPB spatial impact data from USDA Forest Service aerial detection surveys, and remotely sensed host tree density data were used to parameterize the model using a maximum likelihood approach. At both study sites the model was highly accurate (>84%) in predicting annual pattern formation when the model was re-initiated each year with the location of new patches of infested trees. Prediction of annual population growth at both sites was also good (>90%), although the model under-predicted area impacted at the Washington site, and at both sites was unable to predict initiation of new small patches. Our model extends previous research by providing a mechanistic description of the link between motility, dispersal and temperature-dependent MPB phenology. (C) 2013 Elsevier B.V. All rights reserved. C1 [Powell, James A.] Utah State Univ, Dept Math & Stat, Logan, UT 84322 USA. [Powell, James A.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Bentz, Barbara J.] US Forest Serv, USDA, Rocky Mt Res Stn, Logan, UT 84321 USA. RP Powell, JA (reprint author), Utah State Univ, Dept Math & Stat, Logan, UT 84322 USA. EM jim.powell@usu.edu; bbentz@fs.fed.us FU USDA Forest Service; Western Wildland Threat Assessment Center; National Science Foundation [DEB 0918756] FX We thank Jim Vandygriff for assistance with phloem temperature measurements at both study sites. Tom Edwards gave very helpful advice regarding assessing spatial goodness-of-fit. Peter Molnar and Michael Neubert read and commented on earlier drafts, much to our benefit. Funding was provided by the USDA Forest Service, Western Wildland Threat Assessment Center and National Science Foundation, DEB 0918756. NR 81 TC 15 Z9 15 U1 7 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD FEB 10 PY 2014 VL 273 BP 173 EP 185 DI 10.1016/j.ecolmodel.2013.10.034 PG 13 WC Ecology SC Environmental Sciences & Ecology GA AB3EJ UT WOS:000331673800017 ER PT J AU Ehrlich, KC AF Ehrlich, Kenneth C. TI Non-aflatoxigenic Aspergillus flavus to prevent aflatoxin contamination in crops: advantages and limitations SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE aflatoxin; Aspergillus flavus; biocontrol; food safety; recombination; maize; cottonseed; population diversity ID VEGETATIVE COMPATIBILITY GROUPS; SECTION FLAVI; BIOLOGICAL-CONTROL; ENVIRONMENTAL-FACTORS; REDUCTION STRATEGIES; AGROECOLOGICAL-ZONES; MYCOTOXIN PRODUCTION; CYCLOPIAZONIC ACID; GENETIC DIVERSITY; UNITED-STATES AB Aspergillus flavus is a diverse assemblage of strains that include aflatoxin-producing and non-toxigenic strains with cosmopolitan distribution. The most promising strategy currently being used to reduce preharvest contamination of crops with aflatoxin is to introduce non-aflatoxin (biocontrol) A. flavus into the crop environment. Whether or not introduction of biocontrol strains into agricultural fields is enough to reduce aflatoxin contamination to levels required for acceptance of the contaminated food as fit for consumption is still unknown. There is no question that biocontrol strains are able to reduce the size of the populations of aflatoxin-producing strains but the available data suggests that at most only a four- to five-fold reduction in aflatoxin contamination is achieved. There are many challenges facing this strategy that are both short term and long term. First, the population biology of A. flavus is not well understood due in part to A. flavus's diversity, its ability to form heterokaryotic reproductive forms, and its unknown ability to survive for prolonged periods after application. Second, biocontrol strains must be selected that are suitable for the environment, the type of crop, and the soil into which they will be introduced. Third, there is a need to guard against inadvertent introduction of A. flavus strains that could impose an additional burden on food safety and food quality, and fourth, with global warming and resultant changes in the soil nutrients and concomitant microbiome populations, the biocontrol strategy must be sufficiently flexible to adapt to such changes. Understanding genetic variation within strains of A. flavus is important for developing a robust biocontrol strategy and it is unlikely that a "one size fits all" strategy will work for preharvest aflatoxin reduction. C1 USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Ehrlich, KC (reprint author), USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA. EM ken.ehrlich@ars.usda.gov NR 76 TC 17 Z9 18 U1 1 U2 32 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 10 PY 2014 VL 5 AR 50 DI 10.3389/fmicb.2014.00050 PG 9 WC Microbiology SC Microbiology GA AB4VZ UT WOS:000331789300001 PM 24575088 ER PT J AU Adu-Kwarteng, E Sakyi-Dawson, EO Ayernor, GS Truong, VD Shih, FF Daigle, K AF Adu-Kwarteng, Evelyn Sakyi-Dawson, Esther O. Ayernor, George S. Van-Den Truong Shih, Fred F. Daigle, Kim TI VARIABILITY OF SUGARS IN STAPLE-TYPE SWEET POTATO (IPOMOEA BATATAS) CULTIVARS: THE EFFECTS OF HARVEST TIME AND STORAGE SO INTERNATIONAL JOURNAL OF FOOD PROPERTIES LA English DT Article DE Sweet potato (Ipomoea batatas); Soluble sugars; Storage; Harvest timing; Amylase activity ID GENE-EXPRESSION; ALPHA-AMYLASE; STARCH; ROOT; CARBOHYDRATE; SUCROSE; TEMPERATURE; COLOR AB Total soluble sugar content and composition was studied by high performance liquid chromatography in four high dry-matter sweet potato cultivars at 3, 4, and 5 months maturity. Total soluble sugar consisted of sucrose, glucose, and fructose, ranging from 4.10-10.82 g/100 g (dry-weight basis). At harvest, there were significant differences in total soluble sugar due to maturity (p < 0.001) and cultivar (p < 0.05). The highest total soluble sugar contents were in 5-month samples at harvest (7.36-10.34 g/100 g) and 4-month samples after short-term storage under tropical ambient conditions (8.66-10.82 g/100 g). Estimated amylase enzyme activity varied significantly with harvest age (p < 0.05). Although reducing sugar contents were low, fructose levels in 5-month samples increased considerably after storage. C1 [Adu-Kwarteng, Evelyn] Crops Res Inst, Kumasi, Ghana. [Sakyi-Dawson, Esther O.; Ayernor, George S.] Univ Ghana, Dept Nutr & Food Sci, Legon, Ghana. [Van-Den Truong; Shih, Fred F.] N Carolina State Univ, USDA ARS, Dept Food Sci, SAA Food Sci Res Unit, Raleigh, NC 27695 USA. [Daigle, Kim] USDA ARS SRRC, New Orleans, LA USA. RP Adu-Kwarteng, E (reprint author), Crops Res Inst, POB 3785, Kumasi, Ghana. EM evekwart@yahoo.com FU Agricultural Sub-Sector Improvement Project (AgSSIP), The World Bank, Ghana FX The authors gratefully acknowledge the Agricultural Sub-Sector Improvement Project (AgSSIP), The World Bank, Ghana, for providing funding for this study. NR 42 TC 0 Z9 0 U1 5 U2 79 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 1094-2912 J9 INT J FOOD PROP JI Int. J. Food Prop. PD FEB 7 PY 2014 VL 17 IS 2 BP 410 EP 420 DI 10.1080/10942912.2011.642439 PG 11 WC Food Science & Technology SC Food Science & Technology GA 227OZ UT WOS:000325124500016 ER PT J AU Zanne, AE Tank, DC Cornwell, WK Eastman, JM Smith, SA FitzJohn, RG McGlinn, DJ O'Meara, BC Moles, AT Reich, PB Royer, DL Soltis, DE Stevens, PF Westoby, M Wright, IJ Aarssen, L Bertin, RI Calaminus, A Govaerts, R Hemmings, F Leishman, MR Oleksyn, J Soltis, PS Swenson, NG Warman, L Beaulieu, JM AF Zanne, Amy E. Tank, David C. Cornwell, William K. Eastman, Jonathan M. Smith, Stephen A. FitzJohn, Richard G. McGlinn, Daniel J. O'Meara, Brian C. Moles, Angela T. Reich, Peter B. Royer, Dana L. Soltis, Douglas E. Stevens, Peter F. Westoby, Mark Wright, Ian J. Aarssen, Lonnie Bertin, Robert I. Calaminus, Andre Govaerts, Rafael Hemmings, Frank Leishman, Michelle R. Oleksyn, Jacek Soltis, Pamela S. Swenson, Nathan G. Warman, Laura Beaulieu, Jeremy M. TI Three keys to the radiation of angiosperms into freezing environments SO NATURE LA English DT Article ID PLANT HEIGHT; EVOLUTION; CHARACTER; DIVERSIFICATION; DIVERSITY; DATABASE; ECOLOGY; ANATOMY; ORIGIN; GROWTH AB Early flowering plants are thought to have been woody species restricted to warm habitats(1-3). This lineage has since radiated into almost every climate, with manifold growth forms(4). As angiosperms spread and climate changed, they evolved mechanisms to cope with episodic freezing. To explore the evolution of traits underpinning the ability to persist in freezing conditions, we assembled a large species-level database of growth habit (woody or herbaceous; 49,064 species), as well as leaf phenology (evergreen or deciduous), diameter of hydraulic conduits (that is, xylem vessels and tracheids) and climate occupancies (exposure to freezing). To model the evolution of species' traits and climate occupancies, we combined these data with an unparalleled dated molecular phylogeny (32,223 species) for land plants. Here we show that woody clades successfully moved into freezing-prone environments by either possessing transport networks of small safe conduits(5) and/or shutting down hydraulic function by dropping leaves during freezing. Herbaceous species largely avoided freezing periods by senescing cheaply constructed aboveground tissue. Growth habit has long been considered labile(6), but we find that growth habit was less labile than climate occupancy. Additionally, freezing environments were largely filled by lineages that had already become herbs or, when remaining woody, already had small conduits (that is, the trait evolved before the climate occupancy). By contrast, most deciduous woody lineages had an evolutionary shift to seasonally shedding their leaves only after exposure to freezing (that is, the climate occupancy evolved before the trait). For angiosperms to inhabit novel cold environments they had to gain new structural and functional trait solutions; our results suggest that many of these solutions were probably acquired before their foray into the cold. C1 [Zanne, Amy E.] George Washington Univ, Dept Biol Sci, Washington, DC 20052 USA. [Zanne, Amy E.] Missouri Bot Garden, Ctr Conservat & Sustainable Dev, St Louis, MO 63121 USA. [Tank, David C.; Eastman, Jonathan M.] Univ Idaho, Dept Biol Sci, Moscow, ID 83844 USA. [Tank, David C.; Eastman, Jonathan M.] Univ Idaho, Inst Bioinformat & Evolutionary Studies, Moscow, ID 83844 USA. [Cornwell, William K.] Dept Ecol Sci, NL-1081 HV Amsterdam, Netherlands. [Cornwell, William K.; Moles, Angela T.; Hemmings, Frank; Warman, Laura] Univ New S Wales, Evolut & Ecol Res Ctr, Sch Biol Earth & Environm Sci, Sydney, NSW 2052, Australia. [Smith, Stephen A.] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. [FitzJohn, Richard G.] Univ British Columbia, Dept Zool, Vancouver, BC V6T 1Z4, Canada. [FitzJohn, Richard G.] Univ British Columbia, Biodivers Res Ctr, Vancouver, BC V6T 1Z4, Canada. [FitzJohn, Richard G.; Westoby, Mark; Wright, Ian J.; Leishman, Michelle R.] Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia. [McGlinn, Daniel J.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [McGlinn, Daniel J.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [O'Meara, Brian C.] Univ Tennessee, Dept Ecol & Evolutionary Biol, Knoxville, TN 37996 USA. [Reich, Peter B.; Oleksyn, Jacek] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Reich, Peter B.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Royer, Dana L.] Wesleyan Univ, Dept Earth & Environm Sci, Middletown, CT 06459 USA. [Soltis, Douglas E.; Calaminus, Andre] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Soltis, Douglas E.; Soltis, Pamela S.] Univ Florida, Florida Museum Nat Hist, Gainesville, FL 32611 USA. [Soltis, Douglas E.; Soltis, Pamela S.] Univ Florida, Genet Inst, Gainesville, FL 32611 USA. [Stevens, Peter F.] Univ Missouri, Dept Biol, St Louis, MO 63121 USA. [Aarssen, Lonnie] Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada. [Bertin, Robert I.] Coll Holy Cross, Dept Biol, Worcester, MA 01610 USA. [Govaerts, Rafael] Royal Bot Gardens, Richmond TW9 3AB, Surrey, England. [Oleksyn, Jacek] Polish Acad Sci, Inst Dendrol, PL-62035 Kornik, Poland. [Swenson, Nathan G.] Michigan State Univ, Dept Plant Biol & Ecol, E Lansing, MI 48824 USA. [Warman, Laura] US Forest Serv, Inst Pacific Isl Forestry, USDA, Hilo, HI 96720 USA. [Beaulieu, Jeremy M.] Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN 37996 USA. RP Zanne, AE (reprint author), George Washington Univ, Dept Biol Sci, Washington, DC 20052 USA. EM aezanne@gmail.com RI Soltis, Pamela/L-5184-2015; Moles, Angela/C-3083-2008; Leishman, Michelle/G-9726-2012; Wright, Ian/G-4979-2012; Warman, Laura/C-6651-2011; Oleksyn, Jacek/I-4539-2012 OI Smith, Stephen/0000-0003-2035-9531; Moles, Angela/0000-0003-2041-7762; Royer, Dana/0000-0003-0976-953X; Cornwell, Will/0000-0003-4080-4073; O'Meara, Brian/0000-0002-0337-5997; Leishman, Michelle/0000-0003-4830-5797; Wright, Ian/0000-0001-8338-9143; Warman, Laura/0000-0001-7339-749X; FU National Evolutionary Synthesis Center (NESCent), National Science Foundation [EF- 0905606]; Macquarie University Genes to Geoscience Research Centre FX We thank T. Robertson and A. Hahn at the Global Biodiversity Information Facility for providing species' georeference points, A. Ordonez for providing growth form data, and A. Miller and D. Ackerly for helpful comments on a draft of this manuscript. Support for this work was given to the working group "Tempo and Mode of Plant Trait Evolution: Synthesizing Data from Extant and Extinct Taxa" by the National Evolutionary Synthesis Center (NESCent), National Science Foundation grant #EF- 0905606 and Macquarie University Genes to Geoscience Research Centre. NR 29 TC 142 Z9 146 U1 28 U2 248 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD FEB 6 PY 2014 VL 506 IS 7486 BP 89 EP + DI 10.1038/nature12872 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 303BA UT WOS:000330648100037 PM 24362564 ER PT J AU Peloso, GM Auer, PL Bis, JC Voorman, A Morrison, AC Stitziel, NO Brody, JA Khetarpal, SA Crosby, JR Fomage, M Isaacs, A Jakobsdottir, J Feitosa, MF Davies, G Huffman, JE Manichaikul, A Davis, B Lohman, K Joon, AY Smith, AV Grove, ML Zanoni, P Redon, V Demissie, S Lawson, K Peters, U Carlson, C Jackson, RD Ryckman, KK Mackey, RH Robinson, JG Siscovick, DS Schreiner, PJ Mychaleckyj, JC Pankow, JS Holman, A Uitterlinden, AG Harris, TB Taylor, KD Stafford, JM Reynolds, LM Marioni, RE Dehghan, A Franco, OH Patele, AP Lu, YC Hindy, G Gottesman, O Bottinger, EP Melander, O Orho-Melander, M Loos, RJF Duga, S Merlini, PA Farrall, M Goel, A Asselta, R Girelli, D Martinelli, N Shah, SH Kraus, WE Li, MY Rader, DJ Reilly, MP McPherson, R Watkins, H Ardissino, D Zhang, QY Wang, JD Tsai, MY Taylor, HA Correa, A Griswold, ME Lange, LA Starr, JM Rudan, I Eiriksdottir, G Launer, LJ Ordovas, JM Levy, D Chen, YDI Reiner, AP Hayward, C Polasek, O Deary, IJ Borecki, IB Liu, YM Gudnason, V Wilson, JG van Duijn, CM Kooperberg, C Rich, SS Psaty, BM Rotter, JI O'Donnell, CJ Rice, K Boerwinkle, E Kathiresan, S Cupples, LA AF Peloso, Gina M. Auer, Paul L. Bis, Joshua C. Voorman, Arend Morrison, Alanna C. Stitziel, Nathan O. Brody, Jennifer A. Khetarpal, Sumeet A. Crosby, Jacy R. Fomage, Myriam Isaacs, Aaron Jakobsdottir, Johanna Feitosa, Mary F. Davies, Gail Huffman, Jennifer E. Manichaikul, Ani Davis, Brian Lohman, Kurt Joon, Aron Y. Smith, Albert V. Grove, Megan L. Zanoni, Paolo Redon, Valeska Demissie, Serkalem Lawson, Kim Peters, Ulrike Carlson, Christopher Jackson, Rebecca D. Ryckman, Kelli K. Mackey, Rachel H. Robinson, Jennifer G. Siscovick, David S. Schreiner, Pamela J. Mychaleckyj, Josyf C. Pankow, James S. Holman, Albert Uitterlinden, Andre G. Harris, Tamara B. Taylor, Kent D. Stafford, Jeanette M. Reynolds, Lindsay M. Marioni, Riccardo E. Dehghan, Abbas Franco, Oscar H. Patele, Aniruddh P. Lu, Yingchang Hindy, George Gottesman, Omri Bottinger, Erwin P. Melander, Olle Orho-Melander, Marju Loos, Ruth J. F. Duga, Stefano Merlini, Piera Angelica Farrall, Martin Goel, Anuj Asselta, Rosanna Girelli, Domenico Martinelli, Nicola Shah, Svati H. Kraus, William E. Li, Mingyao Rader, Daniel J. Reilly, Muredach P. McPherson, Ruth Watkins, Hugh Ardissino, Diego Zhang, Qunyuan Wang, Judy Tsai, Michael Y. Taylor, Herman A. Correa, Adolfo Griswold, Michael E. Lange, Leslie A. Starr, John M. Rudan, Igor Eiriksdottir, Gudny Launer, Lenore J. Ordovas, Jose M. Levy, Daniel Chen, Y. -D. Ida Reiner, Alexander P. Hayward, Caroline Polasek, Ozren Deary, Ian J. Borecki, Ingrid B. Liu, Yongmei Gudnason, Vilmundur Wilson, James G. van Duijn, Cornelia M. Kooperberg, Charles Rich, Stephen S. Psaty, Bruce M. Rotter, Jerome I. O'Donnell, Christopher J. Rice, Kenneth Boerwinkle, Eric Kathiresan, Sekar Cupples, L. Adrienne CA NHLBI GO Exome Sequencing Project TI Association of Low-Frequency and Rare Coding-Sequence Variants with Blood Lipids and Coronary Heart Disease in 56,000 Whites and Blacks SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Article ID PLATELET-ACTIVATING-FACTOR; DENSITY-LIPOPROTEIN CHOLESTEROL; FACTOR ACETYLHYDROLASES; PCSK9; PROTEIN; TRAITS; LDL AB Low-frequency coding DNA sequence variants in the proprotein convertase subtilisin/kexin type 9 gene (PCSK9) lower plasma low-density lipoprotein cholesterol (LDL-C), protect against risk of coronary heart disease (CHD), and have prompted the development of a new class of therapeutics. It is uncertain whether the PCSK9 example represents a paradigm or an isolated exception. We used the "Exome Array" to genotype >200,000 low-frequency and rare coding sequence variants across the genome in 56,538 individuals (42,208 European ancestry [EA] and 14,330 African ancestry [AA]) and tested these variants for association with LDL-C, high-density lipoprotein cholesterol (HDL-C), and triglycerides. Although we did not identify new genes associated with LDL-C, we did identify four low-frequency (frequencies between 0.1% and 2%) variants (ANGPTL8 rs145464906 [c.361C>T; p.Gln121*], PAFAH1B2 rs186808413 [c.482C>T; p.Ser161Leu], COL18A1 rs114139997 [c.331G>A; p.Gly111Arg], and PCSK7 rs142953140 [c.1511G>A; p.Arg504His]) with large effects on HDL-C and/or triglycerides. None of these four variants was associated with risk for CHD, suggesting that examples of low-frequency coding variants with robust effects on both lipids and CHD will be limited. C1 [Peloso, Gina M.; Patele, Aniruddh P.; Kathiresan, Sekar] Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA. [Peloso, Gina M.; Kathiresan, Sekar] Massachusetts Gen Hosp, Cardiovasc Res Ctr, Boston, MA 02114 USA. [Peloso, Gina M.; O'Donnell, Christopher J.; Kathiresan, Sekar] Harvard Univ, Dept Med, Sch Med, Boston, MA 02115 USA. [Peloso, Gina M.; Patele, Aniruddh P.; Kathiresan, Sekar] Broad Inst, Program Med & Populat Genet, Cambridge, MA 02142 USA. [Auer, Paul L.; Peters, Ulrike; Carlson, Christopher; Reiner, Alexander P.; Kooperberg, Charles] Fred Hutchinson Canc Res Ctr, Publ Hlth Sci Div, Seattle, WA 98109 USA. [Auer, Paul L.] Univ Wisconsin, Sch Publ Hlth, Milwaukee, WI 53201 USA. [Bis, Joshua C.; Brody, Jennifer A.; Siscovick, David S.; Psaty, Bruce M.] Univ Washington, Cardiovasc Hlth Res Unit, Dept Med, Seattle, WA 98101 USA. [Voorman, Arend; Rice, Kenneth] Univ Washington, Dept Biostat, Seattle, WA 98195 USA. [Morrison, Alanna C.; Crosby, Jacy R.; Fomage, Myriam; Davis, Brian; Grove, Megan L.; Lawson, Kim; Boerwinkle, Eric] Univ Texas Hlth Sci Ctr Houston, Sch Publ Hlth, Ctr Human Genet, Houston, TX 77030 USA. [Stitziel, Nathan O.] Washington Univ, Sch Med, Div Cardiovasc, Dept Med, St Louis, MO 63110 USA. [Stitziel, Nathan O.; Feitosa, Mary F.; Zhang, Qunyuan; Wang, Judy; Borecki, Ingrid B.] Washington Univ, Sch Med, Dept Genet, Div Stat Genom, St Louis, MO 63110 USA. [Khetarpal, Sumeet A.; Zanoni, Paolo; Redon, Valeska; Rader, Daniel J.; Reilly, Muredach P.] Univ Penn, Perelman Sch Med, Philadelphia, PA 19104 USA. [Crosby, Jacy R.] Univ Texas Grad Sch Biomed Sci Houston, Dept Biostat Bioinformat & Syst Biol, Houston, TX 77030 USA. [Fomage, Myriam; Joon, Aron Y.] Univ Texas Hlth Sci Ctr Houston, Inst Mol Med, Houston, TX 77030 USA. [Isaacs, Aaron; van Duijn, Cornelia M.] Erasmus Univ, Med Ctr, Dept Epidemiol, Genet Epidemiol Unit, NL-3015 CN Rotterdam, Netherlands. [Jakobsdottir, Johanna; Smith, Albert V.; Eiriksdottir, Gudny; Gudnason, Vilmundur] Iceland Heart Assoc, IS-201 Kopavogur, Iceland. [Davies, Gail; Marioni, Riccardo E.; Starr, John M.; Deary, Ian J.] Univ Edinburgh, Ctr Cognit Ageing & Cognit Epidemiol, Edinburgh EH8 9JZ, Midlothian, Scotland. [Davies, Gail; Marioni, Riccardo E.; Deary, Ian J.] Univ Edinburgh, Dept Psychol, Edinburgh EH8 9JZ, Midlothian, Scotland. [Huffman, Jennifer E.; Hayward, Caroline] Univ Edinburgh, MRC IGMM, MRC Human Genet, Edinburgh EH4 2XU, Midlothian, Scotland. [Manichaikul, Ani; Mychaleckyj, Josyf C.; Rich, Stephen S.] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA 22908 USA. [Manichaikul, Ani; Mychaleckyj, Josyf C.; Rich, Stephen S.] Univ Virginia, Dept Publ Hlth Sci, Charlottesville, VA 22908 USA. [Lohman, Kurt; Stafford, Jeanette M.; Reynolds, Lindsay M.; Liu, Yongmei] Wake Forest Sch Med, Winston Salem, NC 27106 USA. [Smith, Albert V.; Gudnason, Vilmundur] Univ Iceland, Fac Med, IS-101 Reykjavik, Iceland. [Demissie, Serkalem; Cupples, L. Adrienne] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02118 USA. [Demissie, Serkalem; Levy, Daniel; O'Donnell, Christopher J.; Cupples, L. Adrienne] NHLBI, Framingham Heart Study, Framingham, MA 01702 USA. [Jackson, Rebecca D.] Ohio State Univ, Div Endocrinol Diabet & Metab, Dept Internal Med, Columbus, OH 43210 USA. [Ryckman, Kelli K.; Robinson, Jennifer G.] Univ Iowa, Coll Publ Hlth, Dept Epidemiol, Iowa City, IA 52242 USA. [Mackey, Rachel H.] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Epidemiol, Pittsburgh, PA 15261 USA. [Siscovick, David S.; Reiner, Alexander P.; Psaty, Bruce M.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA. [Schreiner, Pamela J.; Pankow, James S.] Univ Minnesota, Div Epidemiol & Community Hlth, Sch Publ Hlth, Minneapolis, MN 55454 USA. [Holman, Albert; Uitterlinden, Andre G.; Dehghan, Abbas; Franco, Oscar H.] Erasmus Univ, Med Ctr, Dept Epidemiol, NL-3015 CN Rotterdam, Netherlands. [Harris, Tamara B.; Launer, Lenore J.] NIA, NIH, Bethesda, MD 20892 USA. [Taylor, Kent D.; Chen, Y. -D. Ida; Rotter, Jerome I.] Harbor UCLA Med Ctr, Inst Translat Genom & Populat Sci, Los Angeles BioMed Res Inst, Torrance, CA 90502 USA. [Patele, Aniruddh P.] Yale Univ, Sch Med, New Haven, CT 06510 USA. [Lu, Yingchang; Gottesman, Omri; Bottinger, Erwin P.; Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Charles Bronfman Inst Personalized Med, New York, NY 10029 USA. [Lu, Yingchang; Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Genet Obes & Related Metab Traits Program, New York, NY 10029 USA. [Hindy, George; Melander, Olle] Lund Univ, Dept Clin Sci Malmo, Clin Res Ctr, S-20502 Malmo, Sweden. [Orho-Melander, Marju] Lund Univ, Malmo Univ Hosp, Dept Clin Sci Diabet & Endocrinol, S-20502 Malmo, Sweden. [Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Mindich Child Hlth & Dev Inst, New York, NY 10029 USA. [Duga, Stefano; Asselta, Rosanna] Univ Milan, Dipartimento Biotecnol Med & Med Traslaz, I-20133 Milan, Italy. [Merlini, Piera Angelica; Ardissino, Diego] ASTC, I-27100 Pavia, Italy. [Merlini, Piera Angelica] Osped Maggiore Niguarda, Div Cardiol, I-20162 Milan, Italy. [Farrall, Martin; Goel, Anuj; Watkins, Hugh] Univ Oxford, Wellcome Trust Ctr Human Genet, Dept Cardiovasc Med, Oxford OX3 7BN, England. [Girelli, Domenico; Martinelli, Nicola] Univ Verona, Sch Med, Dept Med, I-37134 Verona, Italy. [Shah, Svati H.] Duke Univ, Div Urogynecol, Dept Obstet & Gynecol, Durham, NC 27710 USA. [Shah, Svati H.; Kraus, William E.] Duke Univ, Sch Med, Dept Med, Div Cardiol, Durham, NC 27710 USA. [Kraus, William E.] Duke Univ, Sch Med, Duke Mol Physiol Inst, Durham, NC 27701 USA. [Li, Mingyao] Univ Penn, Sch Med, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA. [McPherson, Ruth] Univ Ottawa, Inst Heart, Div Cardiol, Atherogen Lab, Ottawa, ON K1Y 4W7, Canada. [Watkins, Hugh] Merck Sharp & Dohme Corp, Rahway, NJ 07065 USA. [Ardissino, Diego] Azienda Osped Univ Parma, Div Cardiol, I-43100 Parma, Italy. [Tsai, Michael Y.] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55454 USA. [Taylor, Herman A.] Jackson State Univ, Jackson, MS 39217 USA. [Taylor, Herman A.] Tougaloo Coll, Tougaloo, MS 39174 USA. [Taylor, Herman A.; Correa, Adolfo; Griswold, Michael E.] Univ Mississippi, Med Ctr, Dept Med, Jackson, MS 39216 USA. [Lange, Leslie A.] Univ N Carolina, Dept Genet, Chapel Hill, NC 27599 USA. [Starr, John M.] Univ Edinburgh, Alzheimer Scotland Dementia Res Ctr, Edinburgh EH8 9JZ, Midlothian, Scotland. [Rudan, Igor] Univ Edinburgh, Sch Med, Ctr Populat Hlth Sci, Edinburgh EH8 9AG, Midlothian, Scotland. [Rudan, Igor] Univ Split, Croatian Ctr Global Hlth, Fac Med, Split 21000, Croatia. [Ordovas, Jose M.] Natl Ctr Cardiovasc Investigat, Dept Cardiovasc Epidemiol & Populat Genet, Madrid 28049, Spain. [Ordovas, Jose M.] IMDEA Alimentac, Madrid 28049, Spain. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Medford, MA 02155 USA. [Ordovas, Jose M.] NHLBI, NIH, Bethesda, MD 20892 USA. [Polasek, Ozren] Univ Split, Fac Med, Dept Publ Hlth, Split 21000, Croatia. [Wilson, James G.] Univ Mississippi, Dept Physiol & Biophys, Med Ctr, Jackson, MS 39216 USA. [Psaty, Bruce M.] Grp Hlth Cooperat Puget Sound, Grp Hlth Res Inst, Seattle, WA 98101 USA. [Psaty, Bruce M.] Univ Washington, Dept Hlth Serv, Seattle, WA 98195 USA. [O'Donnell, Christopher J.; Kathiresan, Sekar] Massachusetts Gen Hosp, Div Cardiol, Boston, MA 02114 USA. [Boerwinkle, Eric] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA. RP Kathiresan, S (reprint author), Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA. EM skathiresan@partners.org; skathiresan@partners.org; adrienne@bu.edu RI Duga, Stefano/F-8173-2014; Gudnason, Vilmundur/K-6885-2015; Polasek, Ozren/B-6002-2011; Rudan, Igor/I-1467-2012; Smith, Albert/K-5150-2015; Martinelli, Nicola/J-5622-2016; Hayward, Caroline/M-8818-2016; Feitosa, Mary/K-8044-2012; Hindy, George/H-1864-2016; OI Gudnason, Vilmundur/0000-0001-5696-0084; Polasek, Ozren/0000-0002-5765-1862; Rudan, Igor/0000-0001-6993-6884; Smith, Albert/0000-0003-1942-5845; Martinelli, Nicola/0000-0001-6465-5119; Hayward, Caroline/0000-0002-9405-9550; Feitosa, Mary/0000-0002-0933-2410; Asselta, Rosanna/0000-0001-5351-0619; Reynolds, Lindsay/0000-0001-6157-0144; Mackey, Rachel/0000-0001-6088-2664; Hindy, George/0000-0002-7257-9299; Stitziel, Nathan/0000-0002-4963-8211; Manichaikul, Ani/0000-0002-5998-795X; Dehghan, Abbas/0000-0001-6403-016X; Duga, Stefano/0000-0003-3457-1410; Watkins, Hugh/0000-0002-5287-9016; Pankow, James/0000-0001-7076-483X FU National Heart, Lung, and Blood Institute (NHLBI); NHLBI [RC2 HL-103010, RC2 HL-102923, RC2 HL-102924, RC2 HL-102925, RC2 HL-102926, T32HL007208]; National Heart, Lung, and Blood Institute [HL105756]; Massachusetts General Hospital (MGH); Howard Goodman Fellowship from MGH; Donovan Family Foundation [R01HL107816]; Fondation Leducq; NIH [RC2 HL-102925]; Merck; NIH/NHLBI [K08-HL114642]; NWO grant (veni) [916.12.154]; EUR Fellowship FX The authors wish to acknowledge the support of the National Heart, Lung, and Blood Institute (NHLBI) and the contributions of the research institutions, study investigators, field staff, and study participants in creating this resource for biomedical research. Funding for GO ESP was provided by NHLBI grants RC2 HL-103010 (HeartGO), RC2 HL-102923 (LungGO), and RC2 HL-102924 (WHISP). The exome sequencing was performed through NHLBI grants RC2 HL-102925 (BroadGO) and RC2 HL-102926 (SeattleGO). Infrastructure for the CHARGE Consortium is supported, in part, by the National Heart, Lung, and Blood Institute (grant HL105756). G.M.P. is supported by award number T32HL007208 from the NHLBI. S.K. is supported by a Research Scholar award from the Massachusetts General Hospital (MGH), the Howard Goodman Fellowship from MGH, the Donovan Family Foundation, R01HL107816, and a grant from Fondation Leducq. Exome Array genotyping in case-control studies of coronary heart disease was supported by NIH RC2 HL-102925 and an investigator-initiated research grant from Merck to S.K. N.O.S. is supported, in part, by a career development award from the NIH/NHLBI K08-HL114642. A.D. is supported by NWO grant (veni, 916.12.154) and the EUR Fellowship. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NHLBI or NIH. A full listing of acknowledgements is provided in Supplemental Data. NR 28 TC 99 Z9 100 U1 3 U2 23 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0002-9297 EI 1537-6605 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD FEB 6 PY 2014 VL 94 IS 2 BP 223 EP 232 DI 10.1016/j.ajhg.2014.01.009 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA AA9NF UT WOS:000331419500006 PM 24507774 ER PT J AU Bao, ZM Stodghill, PV Myers, CR Lam, H Wei, HL Chakravarthy, S Kvitko, BH Collmer, A Cartinhour, SW Schweitzer, P Swingle, B AF Bao, Zhongmeng Stodghill, Paul V. Myers, Christopher R. Lam, Hanh Wei, Hai-Lei Chakravarthy, Suma Kvitko, Brian H. Collmer, Alan Cartinhour, Samuel W. Schweitzer, Peter Swingle, Bryan TI Genomic Plasticity Enables Phenotypic Variation of Pseudomonas syringae pv. tomato DC3000 SO PLOS ONE LA English DT Article ID SECRETION SYSTEM EFFECTORS; GENE DUPLICATION; III EFFECTORS; ESCHERICHIA-COLI; AVIRULENCE GENES; REGULON MEMBERS; EVOLUTION; PATHOGEN; BACTERIA; IDENTIFICATION AB Whole genome sequencing revealed the presence of a genomic anomaly in the region of 4.7 to 4.9 Mb of the Pseudomonas syringae pv. tomato (Pst) DC3000 genome. The average read depth coverage of Pst DC3000 whole genome sequencing results suggested that a 165 kb segment of the chromosome had doubled in copy number. Further analysis confirmed the 165 kb duplication and that the two copies were arranged as a direct tandem repeat. Examination of the corresponding locus in Pst NCPPB1106, the parent strain of Pst DC3000, suggested that the 165 kb duplication most likely formed after the two strains diverged via transposition of an ISPsy5 insertion sequence (IS) followed by unequal crossing over between ISPsy5 elements at each end of the duplicated region. Deletion of one copy of the 165 kb region demonstrated that the duplication facilitated enhanced growth in some culture conditions, but did not affect pathogenic growth in host tomato plants. These types of chromosomal structures are predicted to be unstable and we have observed resolution of the 165 kb duplication to single copy and its subsequent re-duplication. These data demonstrate the role of IS elements in recombination events that facilitate genomic reorganization in P. syringae. C1 [Bao, Zhongmeng; Lam, Hanh; Wei, Hai-Lei; Chakravarthy, Suma; Collmer, Alan; Cartinhour, Samuel W.; Swingle, Bryan] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Stodghill, Paul V.; Cartinhour, Samuel W.; Swingle, Bryan] ARS, USDA, Ithaca, NY USA. [Myers, Christopher R.] Cornell Univ, Dept Phys, Lab Atom & Solid State Phys, Ithaca, NY 14853 USA. [Kvitko, Brian H.] Michigan State Univ, MSU DOE Plant Res Lab, E Lansing, MI 48824 USA. [Schweitzer, Peter] Cornell Univ, Biotechnol Resource Ctr, Ithaca, NY USA. RP Swingle, B (reprint author), Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. EM Bryan.Swingle@ars.usda.gov FU USDA-ARS; National Science Foundation [IOS-1025642] FX This work was funded by the USDA-ARS. This work was partially supported by National Science Foundation Plant Genome Research Program Grant IOS-1025642. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 64 TC 5 Z9 5 U1 2 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 6 PY 2014 VL 9 IS 2 AR e86628 DI 10.1371/journal.pone.0086628 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AA1BH UT WOS:000330830700010 PM 24516535 ER PT J AU Cao, HP Zhang, L Tan, XF Long, HX Shockey, JM AF Cao, Heping Zhang, Lin Tan, Xiaofeng Long, Hongxu Shockey, Jay M. TI Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii) SO PLOS ONE LA English DT Article ID PLANT ARABIDOPSIS-THALIANA; OIL BODIES; FATTY-ACID; GENOME SEQUENCE; ENDOPLASMIC-RETICULUM; BIODIESEL PRODUCTION; SEED DEVELOPMENT; LIPID DROPLETS; BRASSICA-NAPUS; DRAFT GENOME AB Triacylglycerols (TAG) are the major molecules of energy storage in eukaryotes. TAG are packed in subcellular structures called oil bodies or lipid droplets. Oleosins (OLE) are the major proteins in plant oil bodies. Multiple isoforms of OLE are present in plants such as tung tree (Vernicia fordii), whose seeds are rich in novel TAG with a wide range of industrial applications. The objectives of this study were to identify OLE genes, classify OLE proteins and analyze OLE gene expression in tung trees. We identified five tung tree OLE genes coding for small hydrophobic proteins. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that the five tung OLE genes represented the five OLE subfamilies and all contained the "proline knot'' motif (PX5SPX3P) shared among 65 OLE from 19 tree species, including the sequenced genomes of Prunus persica (peach), Populus trichocarpa (poplar), Ricinus communis (castor bean), Theobroma cacao (cacao) and Vitis vinifera (grapevine). Tung OLE1, OLE2 and OLE3 belong to the S type and OLE4 and OLE5 belong to the SM type of Arabidopsis OLE. TaqMan and SYBR Green qPCR methods were used to study the differential expression of OLE genes in tung tree tissues. Expression results demonstrated that 1) All five OLE genes were expressed in developing tung seeds, leaves and flowers; 2) OLE mRNA levels were much higher in seeds than leaves or flowers; 3) OLE1, OLE2 and OLE3 genes were expressed in tung seeds at much higher levels than OLE4 and OLE5 genes; 4) OLE mRNA levels rapidly increased during seed development; and 5) OLE gene expression was well-coordinated with tung oil accumulation in the seeds. These results suggest that tung OLE genes 1-3 probably play major roles in tung oil accumulation and/or oil body development. Therefore, they might be preferred targets for tung oil engineering in transgenic plants. C1 [Cao, Heping; Shockey, Jay M.] ARS, USDA, So Reg Res Ctr, Commod Utilizat Res Unit, New Orleans, LA 70130 USA. [Zhang, Lin; Tan, Xiaofeng; Long, Hongxu] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha, Hunan, Peoples R China. RP Cao, HP (reprint author), ARS, USDA, So Reg Res Ctr, Commod Utilizat Res Unit, New Orleans, LA 70130 USA. EM Heping.Cao@ars.usda.gov OI Shockey, Jay/0000-0002-5057-5457 FU USDA-Agricultural Research Service Quality and Utilization of Agricultural Products Research Program 306 through CRIS [6435-41000-102-00D, 6435-41000-102-10N]; National Forestry Public Welfare Industry Research Project of China [201204403] FX This work was supported by the USDA-Agricultural Research Service Quality and Utilization of Agricultural Products Research Program 306 through CRIS 6435-41000-102-00D and 6435-41000-102-10N and National Forestry Public Welfare Industry Research Project of China (201204403). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 89 TC 5 Z9 5 U1 3 U2 35 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 6 PY 2014 VL 9 IS 2 AR e88409 DI 10.1371/journal.pone.0088409 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AA1BH UT WOS:000330830700069 PM 24516650 ER PT J AU Liu, H Bayer, M Druka, A Russell, JR Hackett, CA Poland, J Ramsay, L Hedley, PE Waugh, R AF Liu, Hui Bayer, Micha Druka, Arnis Russell, Joanne R. Hackett, Christine A. Poland, Jesse Ramsay, Luke Hedley, Pete E. Waugh, Robbie TI An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley SO BMC GENOMICS LA English DT Article DE Barley; Dwarfing gene; Genotyping by sequencing; Physical map ID MAPPING POPULATION; GOLDEN PROMISE; SALT TOLERANCE; SNP DISCOVERY; LINKAGE MAP; GENOME; GENE; CONSTRUCTION; MUTATION; MUTANTS AB We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from a cross between the two-rowed barley cultivar 'Golden Promise' (ari-e.GP/Vrs1) and the six-rowed cultivar 'Morex' (Ari-e/vrs1) to map plant height. We identified three Quantitative Trait Loci (QTL), the first in a region encompassing the spike architecture gene Vrs1 on chromosome 2H, the second in an uncharacterised centromeric region on chromosome 3H, and the third in a region of chromosome 5H coinciding with the previously described dwarfing gene Breviaristatum-e (Ari-e). Background: Barley cultivars in North-western Europe largely contain either of two dwarfing genes; Denso on chromosome 3H, a presumed ortholog of the rice green revolution gene OsSd1, or Breviaristatum-e (ari-e) on chromosome 5H. A recessive mutant allele of the latter gene, ari-e.GP, was introduced into cultivation via the cv. 'Golden Promise' that was a favourite of the Scottish malt whisky industry for many years and is still used in agriculture today. Results: Using GBS mapping data and phenotypic measurements we show that ari-e.GP maps to a small genetic interval on chromosome 5H and that alternative alleles at a region encompassing Vrs1 on 2H along with a region on chromosome 3H also influence plant height. The location of Ari-e is supported by analysis of near-isogenic lines containing different ari-e alleles. We explored use of the GBS to populate the region with sequence contigs from the recently released physically and genetically integrated barley genome sequence assembly as a step towards Ari-e gene identification. Conclusions: GBS was an effective and relatively low-cost approach to rapidly construct a genetic map of the GPMx population that was suitable for genetic analysis of row type and height traits, allowing us to precisely position ari-e.GP on chromosome 5H. Mapping resolution was lower than we anticipated. We found the GBS data more complex to analyse than other data types but it did directly provide linked SNP markers for subsequent higher resolution genetic analysis. C1 [Liu, Hui; Bayer, Micha; Druka, Arnis; Russell, Joanne R.; Ramsay, Luke; Hedley, Pete E.; Waugh, Robbie] James Hutton Inst, Dundee DD2 5DA, Scotland. [Hackett, Christine A.] Biomath & Stat Scotland BioSS, Dundee DD2 5DA, Scotland. [Poland, Jesse] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Poland, Jesse] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Liu, Hui] Univ St Andrews, St Andrews KY16 9ST, Fife, Scotland. [Waugh, Robbie] Univ Dundee, Coll Life Sci, James Hutton Inst, Div Plant Sci, Dundee DD2 5DA, Scotland. RP Waugh, R (reprint author), James Hutton Inst, Dundee DD2 5DA, Scotland. EM robbie.waugh@hutton.ac.uk OI Poland, Jesse/0000-0002-7856-1399 FU EU; Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS) FX We would like to acknowledge Jill Alexander, Nicola Bonar, Richard Keith, Pauline Smith and Ilze Druka for excellent technical assistance, in particular during population development and phenotypic analysis. The majority of the funding for the described research came from the EU FP7 project 'TriticeaeGenome' and the Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS) Strategic Research Programme Workpackage 5.2. NR 36 TC 42 Z9 43 U1 8 U2 67 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 6 PY 2014 VL 15 AR 104 DI 10.1186/1471-2164-15-104 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AA5EB UT WOS:000331117800001 PM 24498911 ER PT J AU Tsen, SWD Kingsley, DH Poweleit, C Achilefu, S Soroka, DS Wu, TC Tsen, KT AF Tsen, Shaw-Wei D. Kingsley, David H. Poweleit, Christian Achilefu, Samuel Soroka, Douglas S. Wu, T. C. Tsen, Kong-Thon TI Studies of inactivation mechanism of non-enveloped icosahedral virus by a visible ultrashort pulsed laser SO VIROLOGY JOURNAL LA English DT Article ID COHERENT-PHONON OSCILLATIONS; SCATTERING; EXCITATION; NOROVIRUS; WAVES; LIGHT AB Background: Low-power ultrashort pulsed (USP) lasers operating at wavelengths of 425 nm and near infrared region have been shown to effectively inactivate viruses such as human immunodeficiency virus (HIV), M13 bacteriophage, and murine cytomegalovirus (MCMV). It was shown previously that non-enveloped, helical viruses such as M13 bacteriophage, were inactivated by a USP laser through an impulsive stimulated Raman scattering (ISRS) process. Recently, enveloped virus like MCMV has been shown to be inactivated by a USP laser via protein aggregation induced by an ISRS process. However, the inactivation mechanism for a clinically important class of viruses - non-enveloped, icosahedral viruses remains unknown. Results and discussions: We have ruled out the following four possible inactivation mechanisms for non-enveloped, icosahedral viruses, namely, (1) inactivation due to ultraviolet C (UVC) photons produced by non-linear optical process of the intense, fundamental laser beam at 425 nm; (2) inactivation caused by thermal heating generated by the direct laser absorption/heating of the virion; (3) inactivation resulting from a one-photon absorption process via chromophores such as porphyrin molecules, or indicator dyes, potentially producing reactive oxygen or other species; (4) inactivation by the USP lasers in which the extremely intense laser pulse produces shock wave-like vibrations upon impact with the viral particle. We present data which support that the inactivation mechanism for non-enveloped, icosahedral viruses is the impulsive stimulated Raman scattering process. Real-time PCR experiments show that, within the amplicon size of 273 bp tested, there is no damage on the genome of MNV 1 caused by the USP laser irradiation. Conclusion: We conclude that our model non-enveloped virus, MNV-1, is inactivated by the ISRS process. These studies provide fundamental knowledge on photon-virus interactions on femtosecond time scales. From the analysis of the transmission electron microscope (TEM) images of viral particles before and after USP laser irradiation, the locations of weak structural links on the capsid of MNV-1 were revealed. This important information will greatly aid our understanding of the structure of non-enveloped, icosahedral viruses. We envision that this non-invasive, efficient viral eradication method will find applications in the disinfection of pharmaceuticals, biologicals and blood products in the near future. C1 [Tsen, Shaw-Wei D.; Achilefu, Samuel] Washington Univ, Sch Med, Dept Radiol, St Louis, MO 63110 USA. [Kingsley, David H.] Delaware State Univ, ARS, USDA, Food Safety & Intervent Technol Res Unit,James WW, Dover, DE 19901 USA. [Poweleit, Christian; Tsen, Kong-Thon] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA. [Achilefu, Samuel] Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA. [Achilefu, Samuel] Washington Univ, Sch Med, Dept Biomed Engn, St Louis, MO 63110 USA. [Soroka, Douglas S.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Wu, T. C.] Johns Hopkins Sch Med, Dept Pathol, Baltimore, MD 21231 USA. [Wu, T. C.] Johns Hopkins Sch Med, Dept Oncol, Baltimore, MD 21231 USA. [Wu, T. C.] Johns Hopkins Sch Med, Dept Obstet, Baltimore, MD 21231 USA. [Wu, T. C.] Johns Hopkins Sch Med, Dept Gynecol, Baltimore, MD 21231 USA. [Wu, T. C.] Johns Hopkins Sch Med, Dept Med Microbiol, Baltimore, MD 21231 USA. [Wu, T. C.] Johns Hopkins Sch Med, Dept Immunol, Baltimore, MD 21231 USA. [Tsen, Kong-Thon] Arizona State Univ, Ctr Biophys, Tempe, AZ 85287 USA. RP Tsen, KT (reprint author), Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA. EM tsen@asu.edu OI Achilefu, Samuel/0000-0002-3133-6717 FU United States Department of Agriculture, Agricultural Research Service; NHI BI Ruth L. Kirschstein NRSA F30 [HI 116183-01] FX The authors thank Gloria Meade (USDA Dover) for technical assistance, and Arisitides Marcano (Delaware State Univ., Dover, DE) Gary Richards (USDA Dover, DE), and Chao Chen (Indiana Univ, Bloomington IN) for technical review of this manuscript. This work was supported by intramural funding from the United States Department of Agriculture, Agricultural Research Service and NHI BI Ruth L. Kirschstein NRSA F30 grant HI 116183-01 (Shaw-Wei D. Tsen). NR 31 TC 5 Z9 5 U1 1 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-422X J9 VIROL J JI Virol. J. PD FEB 5 PY 2014 VL 11 AR 20 DI 10.1186/1743-422X-11-20 PG 9 WC Virology SC Virology GA AF7BA UT WOS:000334867200001 PM 24495489 ER PT J AU Johnson, DM AF Johnson, David M. TI An assessment of pre- and within-season remotely sensed variables for forecasting corn and soybean yields in the United States SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Crop yield forecasting; Corn; Soybeans; Precipitation; NDVI; Land surface temperature; MODIS; Regression tree modeling ID LAND-SURFACE TEMPERATURE; REAL-TIME ESTIMATION; CROP YIELD; WHEAT YIELD; VEGETATION INDEXES; GRAIN-YIELD; ESTIMATION MODEL; RADAR RAINFALL; SATELLITE DATA; CLIMATE-CHANGE AB Four timely and broadly available remotely sensed datasets were assessed for inclusion into county-level corn and soybean yield forecasting efforts focused on the Corn Belt region of the central United States (US). Those datasets were the (1) Normalized Difference Vegetation Index (NDVI) as derived from the Terra satellite's Moderate Resolution Imaging Spectroradiometer (MODIS), (2) daytime and (3) nighttime land surface temperature (LST) as derived from Aqua satellite's MODIS, and (4) precipitation from the National Weather Service (NWS) Nexrad-based gridded data product. The originating MODIS data utilized were the globally produced 8-day, clear sky composited science products (MOD09Q1 and MYD11A2), while the US-wide NWS data were manipulated to mesh with the MODIS imagery both spatially and temporally by regridding and summing the otherwise daily measurements. The crop growing seasons of 2006-2011 were analyzed with each year bounded by 32 8-day periods from mid-February through late October. Land cover classifications known as the Cropland Data Layer as produced annually by the National Agricultural Statistics Service (NASS) were used to isolate the input dataset pixels as to corn and soybeans for each of the corresponding years. The relevant pixels were then averaged by crop and time period to produce a county-level estimate of NDVI, the LSTs, and precipitation. They in turn were related to official annual NASS county level yield statistics. For the Corn Belt region as a whole, both corn and soybean yields were found to be positively correlated with NDVI in the middle of the summer and negatively correlated to daytime LST at that same time. Nighttime LST and precipitation showed no correlations to yield, regardless of the time prior or during the growing season. There was also slight suggestion of low NDVI and high daytime LST in the spring being positively related to final yields, again for both crops. Taking only NDVI and daytime LST as inputs from the 2006-2011 dataset, regression tree-based models were built and county-level, within-sample coefficients of determination (R-2) of 0.93 were found for both crops. Limiting the models by systematically removing late season data showed the model performance to remain strong even at mid-season and still viable even earlier. Finally, the derived models were used to predict out-of-sample for the 2012 season, which ended up having an anomalous drought Yet, the county-level results compared reasonably well against official statistics with R-2 = 0.77 for corn and 0.71 for soybeans. The root-mean-square errors were 1.26 and 0.42 metric tons per hectare, respectively. (C) 2013 Elsevier Inc. All rights reserved. C1 Natl Agr Stat Serv, USDA, Fairfax, VA 22030 USA. RP Johnson, DM (reprint author), Natl Agr Stat Serv, USDA, 3251 Old Lee Highway,Suite 305, Fairfax, VA 22030 USA. EM dave.johnson@nass.usda.gov NR 66 TC 37 Z9 37 U1 8 U2 63 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB 5 PY 2014 VL 141 BP 116 EP 128 DI 10.1016/j.rse.2013.10.027 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AB3AB UT WOS:000331662600010 ER PT J AU Huang, CQ Peng, Y Lang, MG Yeo, IY McCarty, G AF Huang, Chengquan Peng, Yi Lang, Megan Yeo, In-Young McCarty, Greg TI Wetland inundation mapping and change monitoring using Landsat and airborne LiDAR data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Forested wetlands; Subpixel inundation percentage (SIP); Change; Landsat; LiDAR ID SYNTHETIC-APERTURE RADAR; MID-ATLANTIC REGION; TM TASSELED CAP; COVER CLASSIFICATION; FORESTED WETLANDS; INVENTORY MAPS; COASTAL-PLAIN; IMAGERY; REFLECTANCE; VEGETATION AB This paper presents a new approach for mapping wetland inundation change using Landsat and LiDAR intensity data. In this approach, LiDAR data were used to derive highly accurate reference subpixel inundation percentage (SIP) maps at the 30-m resolution. The reference SIP maps were then used to establish statistical relationships between SIP and Landsat data. Inundation changes were mapped by applying the derived relationships to Landsat images acquired in different years. This approach was applied to the upper Choptank River sub-watershed to map wetland inundation for average (2005 and 2007), dry (2009), and wet (2010) years. The derived SIP maps revealed large changes in wetland inundation among dry, average, and wet years. Total areas of near complete inundation (SIP > 75%) and high inundation (SIP between 50% and 75%) in the wet year of 2010 were about five and three times of those in the dry year of 2009, respectively. The wet year also had more medium inundated areas (SIP between 25% and 50%) than the average and dry years, but low inundated areas (SIP < 25%) did not have any particular trend. The mapped inundation changes were found correlated with local drought conditions and stream flow, with the near complete inundated and highly inundated areas having the highest correlations. Given the fact that Landsat are globally available and LiDAR data are becoming increasingly more affordable and available, the approach developed in this study has potential for deriving historical inundation changes over the past decades and for monitoring ongoing changes over much larger areas than demonstrated in this study. (C) 2013 Elsevier Inc. All rights reserved. C1 [Huang, Chengquan; Peng, Yi; Yeo, In-Young] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Lang, Megan] US Forest Serv, USDA, No Res Stn, Beltsville, MD 20705 USA. [McCarty, Greg] USDA ARS, Remote Sensing & Hydrol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Yeo, IY (reprint author), Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. OI Huang, Chengquan/0000-0003-0055-9798 FU NASA [NNX12AG21G]; NASA; NSF-UMD; Wetland Component of the USDA National Conservation Effects Assessment Project; US Geological Survey; USDA Forest Service FX This research was funded by the NASA's Land Cover and Land Use Change Program (contract No: NNX12AG21G). Additional support was provided by the NASA's Terrestrial Ecology, Carbon Cycle Sciences, MEASURES programs, NSF-UMD advance program, the Wetland Component of the USDA National Conservation Effects Assessment Project, US Geological Survey, and USDA Forest Service. Comments provided by two anonymous reviewers are highly appreciated. NR 66 TC 25 Z9 27 U1 3 U2 69 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB 5 PY 2014 VL 141 BP 231 EP 242 DI 10.1016/j.rse.2013.10.020 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AB3AB UT WOS:000331662600019 ER PT J AU Fountain, JC Scully, B Ni, XZ Kemerait, RC Lee, RD Chen, ZY Guo, BZ AF Fountain, Jake C. Scully, BrianT. Ni, Xinzhi Kemerait, Robert C. Lee, Robert D. Chen, Zhi-Yuan Guo, Baozhu TI Environmental influences on maize-Aspergillus flavus interactions and aflatoxin production SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE maize; aflatoxin contamination; environment effects; oxidative stress; host resistance ID QUANTITATIVE TRAIT LOCI; WRKY53 TRANSCRIPTION FACTOR; INSECT HERBIVORY; OXIDATIVE STRESS; ALPHA-AMYLASE; MYCOTOXIN PRODUCTION; MOLECULAR-CLONING; PLANT-RESPONSES; GENE-EXPRESSION; CORN KERNELS AB Since the early 1960s, the fungal pathogen Aspergillus flavus (Link ex Fr.) has been the focus of intensive research due to the production of carcinogenic and highly toxic secondary metabolites collectively known as aflatoxins following pre-harvest colonization of crops. Given this recurrent problem and the occurrence of a severe aflatoxin outbreak in maize (Zea mays L.), particularly in the Southeast U. S. in the 1977 growing season, a significant research effort has been put forth to determine the nature of the interaction occurring between aflatoxin production, A. flavus, environment and its various hosts before harvest. Many studies have investigated this interaction at the genetic, transcript, and protein levels, and in terms of fungal biology at either pre- or post-harvest time points. Later experiments have indicated that the interaction and overall resistance phenotype of the host is a quantitative trait with a relatively low heritability. In addition, a high degree of environmental interaction has been noted, particularly with sources of abiotic stress for either the host or the fungus such as drought or heat stresses. Here, we review the history of research into this complex interaction and propose future directions for elucidating the relationship between resistance and susceptibility to A. flavus colonization, abiotic stress, and its relationship to oxidative stress in which aflatoxin production may function as a form of antioxidant protection to the producing fungus. C1 [Fountain, Jake C.; Kemerait, Robert C.; Guo, Baozhu] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Scully, BrianT.; Guo, Baozhu] ARS, Crop Protect & Management Res Unit, USDA, Tifton, GA 31793 USA. [Ni, Xinzhi] ARS, Crop Genet & Breeding Res Unit, USDA, Tifton, GA 31793 USA. [Lee, Robert D.] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA USA. [Chen, Zhi-Yuan] Louisiana State Univ, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. RP Guo, BZ (reprint author), ARS, Crop Protect & Management Res Unit, USDA, 2747 Davis Rd, Tifton, GA 31793 USA. EM baozhu.guo@ars.usda.gov FU U.S. Department of Agriculture Agricultural Research Service (USDA-ARS); Georgia Agricultural Commodity Commission for Corn; AMCOE (Aflatoxin Mitigation Center of Excellence) FX This work is partially supported by the U.S. Department of Agriculture Agricultural Research Service (USDA-ARS), the Georgia Agricultural Commodity Commission for Corn, and AMCOE (Aflatoxin Mitigation Center of Excellence). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 86 TC 12 Z9 13 U1 11 U2 65 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 5 PY 2014 VL 5 AR 40 DI 10.3389/fmicb.2014.00040 PG 7 WC Microbiology SC Microbiology GA AB4CH UT WOS:000331736200001 PM 24550905 ER PT J AU Moore, GG AF Moore, Geromy G. TI Sex and recombination in aflatoxigenic Aspergilli: global implications SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE Aspergillus; aflatoxin; sexuality; recombination; climate change; biocontrol ID GENE-CLUSTER; DEVELOPING-COUNTRIES; FLAVUS; PARASITICUS; CONTAMINATION; REPRODUCTION; POPULATION; MYCOTOXINS; HEALTH; NOMIUS AB For most of the half century that aflatoxigenic species have been intensively studied, these molds were known only to reproduce asexually, with parasexuality found only in the laboratory between certain mutant strains. Therefore, the fairly recent discovery of their sexual (teleomorphic) states creates a new wrinkle in our understanding of the field behavior of these agriculturally significant fungi. Sex within populations of these fungi, and attendant genetic recombination, eventually may create difficulties for their control; and subsequently for the protection of important human and animal food supplies. Moreover, if fungal sex is a form of response to ecological and environmental stressors, then perhaps human influence and climate change could accelerate this phenomenon. This article will explore scientific research into sexuality and recombination in aflatoxigenic Aspergillus species; the potential impacts these phenomena could have on a popular method of pre-harvest prevention of aflatoxin contamination (i.e., use of non-aflatoxigenic A. flavus for biocontrol); and the outlook for maintaining control of aflatoxin contamination in an era of changing global climate. C1 ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Moore, GG (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM geromy.moore@ars.usda.gov NR 41 TC 7 Z9 7 U1 0 U2 16 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 5 PY 2014 VL 5 AR 32 DI 10.3389/fmicb.2014.00032 PG 5 WC Microbiology SC Microbiology GA AB4BU UT WOS:000331734900002 PM 24550903 ER PT J AU Yi, J Lam, TI Yokoyama, W Cheng, LW Zhong, F AF Yi, Jiang Lam, Tina I. Yokoyama, Wallace Cheng, Luisa W. Zhong, Fang TI Cellular Uptake of beta-Carotene from Protein Stabilized Solid Lipid Nanoparticles Prepared by Homogenization-Evaporation Method SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE beta-carotene; nanoparticles; stability; Caco-2; toxicity; uptake ID FOOD-GRADE NANOEMULSIONS; SOY PROTEIN; CHEMICAL-STABILITY; VITAMIN-A; EMULSIONS; BIOACCESSIBILITY; NANODISPERSIONS; DELIVERY; NUTRACEUTICALS; CYTOTOXICITY AB With a homogenization-evaporation method, beta-carotene (BC) loaded nanoparticles were prepared with different ratios of food-grade sodium caseinate (SC), whey protein isolate (WPI), or soy protein isolate (SPI) to BC and evaluated for their physiochemical stability, in vitro cytotoxicity, and cellular uptake by Caco-2 cells. The particle diameters of the BC loaded nanoparticles with 0.75% SC or 1.0% WPI emulsifiers were 75 and 90 nm, respectively. Mean particle diameters of three BC loaded nanoparticle nanoemulsions increased less than 10% at 4 degrees C while they increased more at 25 degrees C (10-76%) during 30 days of storage. The oxidative stability of BC loaded nanoparticles encapsulated by proteins decreased in the following order: SC > WPI > SPI. The retention rates of BC in nanoparticles were 63.5%, 60.5%, and 41.8% for SC, WPI, and SPI, respectively, after 30 days of storage at 25 degrees C. The BC's chemical stability was improved by increasing the concentration of protein. Both the rate of particle growth and the total BC loss at 25 degrees C were larger than at 4 degrees C. The color of BC loaded nanoparticles decreased with increasing storage in the dark without oxygen, similar to the decrease in BC content of nanoparticles at 4 and 25 degrees C. Almost no cytotoxicity due to BC loaded nanoparticles cellular uptake was observed, especially when diluted 10 times or more. The uptake of BC was significantly improved through nanoparticle delivery systems by 2.6-, 3.4-, and 1.7-fold increase, respectively, for SC, WPI, and SPI, as compared to the free BC. The results of this study indicate that protein stabilized, BC loaded nanoparticles can improve stability and uptake of BC. C1 [Yi, Jiang; Zhong, Fang] Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China. [Yi, Jiang; Lam, Tina I.; Yokoyama, Wallace; Cheng, Luisa W.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Zhong, F (reprint author), Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, 1800 Lihu Ave, Wuxi 214122, Peoples R China. EM fzhong@jiangnan.edu.cn FU National 125 Program [2011BAD23B02, 2013AA1022207]; NSFC [31171686]; 111 Project [B07029, PCSIRT0627]; [NSF-Jiangsu-BK2012556] FX This work was financially supported by National 125 Program 2011BAD23B02, 2013AA1022207; NSFC 31171686; NSF-Jiangsu-BK2012556; 111 Project B07029 and PCSIRT0627. NR 44 TC 21 Z9 21 U1 11 U2 56 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 5 PY 2014 VL 62 IS 5 BP 1096 EP 1104 DI 10.1021/jf404073c PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AA3TH UT WOS:000331014700013 PM 24422504 ER PT J AU Lupton, SJ Huwe, JK Smith, DJ Dearfield, KL Johnston, JJ AF Lupton, Sara J. Huwe, Janice K. Smith, David J. Dearfield, Kerry L. Johnston, John J. TI Distribution and Excretion of Perfluorooctane Sulfonate (PFOS) in Beef Cattle (Bos taurus) SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE perfluorooctane sulfonate; beef cattle; tissue distribution; elimination; residues ID CHICKENS GALLUS-GALLUS; PERFLUORINATED COMPOUNDS; PERFLUOROALKYL CONTAMINANTS; TISSUE DISTRIBUTION; DIETARY-INTAKE; EXPOSURE; TOXICITY; FOODS; ACIDS; ABSORPTION AB Perfluorooctane sulfonate (PFOS), a perfluoroalkyl surfactant used in many industrial products, is present in industrial wastes and in wastewater treatment plant biosolids. Biosolids are commonly applied to pastures and crops used for animal feed; consequently, PFOS may accumulate in the edible tissues of grazing animals or in animals exposed to contaminated feeds. There are no data on the absorption, distribution, and excretion of PFOS in beef cattle, so a 28-day study was conducted to determine these parameters for PFOS in three Lowline Angus steers given a single oral dose of PFOS at approximately 8 mg/kg body weight. PFOS concentrations were determined by liquid chromatography-tandem mass spectrometry in multiple tissue compartments. The major route of excretion was in the feces (11 +/- 1.3% of the dose, mean +/- standard deviation) with minimal PFOS elimination in urine (0.5 +/- 0.07% of the dose). At day 28 the mean plasma concentration remained elevated at 52.6 +/- 3.4 mu g/mL, and it was estimated that 35.8 +/- 4.3% of the dose was present in the plasma. Plasma half-lives could not be calculated due to multiple peaks caused by apparent redistributions from other tissues. These data indicate that after an acute exposure PFOS persists and accumulates in edible tissues. The largest PFOS body burdens were in the blood (similar to 36%), carcass remainder (5.7 +/- 1.6%), and the muscle (4.3 +/- 0.6%). It was concluded that PFOS would accumulate in edible tissues of beef, which Could be a source of exposure for humans. C1 [Lupton, Sara J.; Huwe, Janice K.; Smith, David J.] ARS, Biosci Res Lab, USDA, Fargo, ND 58102 USA. [Dearfield, Kerry L.] USDA, Off Publ Hlth Sci, FSIS, Washington, DC 20250 USA. [Johnston, John J.] USDA, Off Publ Hlth Sci, FSIS, Ft Collins, CO 80526 USA. RP Lupton, SJ (reprint author), ARS, Biosci Res Lab, USDA, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM Sara.Lupton@ars.usda.gov OI Lupton, Sara/0000-0002-4566-595X FU FSIS-ARS [FSIS-IA-9-102] FX Partial funding for this study was provided via an FSIS-ARS Interagency Agreement FSIS-IA-9-102. NR 45 TC 9 Z9 10 U1 1 U2 34 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 5 PY 2014 VL 62 IS 5 BP 1167 EP 1173 DI 10.1021/jf404355b PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AA3TH UT WOS:000331014700021 PM 24443932 ER PT J AU Perazzolli, M Malacarne, G Baldo, A Righetti, L Bailey, A Fontana, P Velasco, R Malnoy, M AF Perazzolli, Michele Malacarne, Giulia Baldo, Angela Righetti, Laura Bailey, Aubrey Fontana, Paolo Velasco, Riccardo Malnoy, Mickael TI Characterization of Resistance Gene Analogues (RGAs) in Apple (Malus x domestica Borkh.) and Their Evolutionary History of the Rosaceae Family SO PLOS ONE LA English DT Article ID NUCLEOTIDE-BINDING SITE; QUANTITATIVE TRAIT LOCI; GENOME-WIDE IDENTIFICATION; FIRE BLIGHT RESISTANCE; RICH REPEAT GENES; NBS-LRR GENES; DISEASE-RESISTANCE; SCAB RESISTANCE; VENTURIA-INAEQUALIS; APHID RESISTANCE AB The family of resistance gene analogues (RGAs) with a nucleotide-binding site (NBS) domain accounts for the largest number of disease resistance genes and is one of the largest gene families in plants. We have identified 868 RGAs in the genome of the apple (Malus x domestica Borkh.) cultivar 'Golden Delicious'. This represents 1.51% of the total number of predicted genes for this cultivar. Several evolutionary features are pronounced in M. domestica, including a high fraction (80%) of RGAs occurring in clusters. This suggests frequent tandem duplication and ectopic translocation events. Of the identified RGAs, 56% are located preferentially on six chromosomes (Chr 2, 7, 8, 10, 11, and 15), and 25% are located on Chr 2. TIR-NBS and non-TIR-NBS classes of RGAs are primarily exclusive of different chromosomes, and 99% of non-TIR-NBS RGAs are located on Chr 11. A phylogenetic reconstruction was conducted to study the evolution of RGAs in the Rosaceae family. More than 1400 RGAs were identified in six species based on their NBS domain, and a neighbor-joining analysis was used to reconstruct the phylogenetic relationships among the protein sequences. Specific phylogenetic clades were found for RGAs of Malus, Fragaria, and Rosa, indicating genus-specific evolution of resistance genes. However, strikingly similar RGAs were shared in Malus, Pyrus, and Prunus, indicating high conservation of specific RGAs and suggesting a monophyletic origin of these three genera. C1 [Perazzolli, Michele; Malacarne, Giulia; Righetti, Laura; Fontana, Paolo; Velasco, Riccardo; Malnoy, Mickael] Fdn Edmund Mach, Res & Innovat Ctr, San Michele All Adige, Italy. [Baldo, Angela; Bailey, Aubrey] USDA ARS, Plant Genet Resources Unit, Geneva, NY USA. RP Malnoy, M (reprint author), Fdn Edmund Mach, Res & Innovat Ctr, San Michele All Adige, Italy. EM mickael.malnoy@fmach.it RI Perazzolli, Michele/B-3636-2012; malnoy, mickael/C-5007-2012; Malacarne, Giulia/N-4244-2015 OI Perazzolli, Michele/0000-0001-7218-9963; Malacarne, Giulia/0000-0003-1439-5958 FU Provincia Autonoma di Trento FX The research was supported by the Provincia Autonoma di Trento. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 80 TC 17 Z9 18 U1 3 U2 54 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 5 PY 2014 VL 9 IS 2 AR e83844 DI 10.1371/journal.pone.0083844 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AA1AT UT WOS:000330829200006 PM 24505246 ER PT J AU Sun, C VanRaden, PM AF Sun, Chuanyu VanRaden, Paul M. TI Increasing Long-Term Response by Selecting for Favorable Minor Alleles SO PLOS ONE LA English DT Article ID GENOMIC SELECTION; ACCURACY AB Long-term response of genomic selection can be improved by considering allele frequencies of selected markers or quantitative trait loci (QTLs). A previous formula to weight allele frequency of favorable minor alleles was tested, and 2 new formulas were developed. The previous formula used nonlinear weights based on square root of frequency of the favorable allele. The new formulas included a parameter delta to balance long- and short-term progress; one used square root and the other used simple linear weights. The formulas were tested by simulation of 20 generations (population size of 3,000 for each generation) with direct selection on 3,000 QTLs (100 per chromosome). A QTL distribution with normally distributed allele effects and a heavy-tailed distribution were tested. Optimum delta from simulation was applied to data from Holstein, Jersey and Brown Swiss dairy cattle to compare differences of adjusted and official genomic evaluations. From simulation, optimum delta was 0.4 for the heavy-tailed QTL distribution but only 0.1 or 0.2 for a normal distribution. The previous formula had slower response than unweighted selection in early generations and did not recover by generation 20. Long-term response was slightly greater with the new formulas than with unweighted selection; the linear formula may be best for routine use because of more progress in early generations compared to nonlinear formula. Official and adjusted U. S. evaluations based on actual genotypes and estimated marker effects were correlated by 0.994 for Holsteins and Jerseys and 0.989 for Brown Swiss using linear weighting of allele frequency, which was higher than nonlinear weighting. The difference between adjusted and official evaluations was highly correlated negatively with an animal's average genomic relationship to the population. Thus, strategies to reduce genomic inbreeding may achieve almost as much long-term progress as selection of favorable minor alleles. C1 [Sun, Chuanyu] Natl Assoc Anim Breeders, Columbia, MO 65205 USA. [VanRaden, Paul M.] ARS, USDA, Anim Improvement Programs Lab, Beltsville, MD USA. RP Sun, C (reprint author), Natl Assoc Anim Breeders, Columbia, MO 65205 USA. EM chuanyu.sun@ars.usda.gov FU National Association of Animal Breeders, United States of America; Animal Improvement Programs Laboratory of Agricultural research service in United States Department of Agriculture FX The work was performed in the project funded regularly by National Association of Animal Breeders, United States of America (http://www.naab-css.org/) and Animal Improvement Programs Laboratory of Agricultural research service in United States Department of Agriculture (http://aipl.arsusda.gov/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 17 TC 0 Z9 0 U1 1 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 5 PY 2014 VL 9 IS 2 AR e88510 DI 10.1371/journal.pone.0088510 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AA1AT UT WOS:000330829200195 PM 24505495 ER PT J AU Calla, B Hall, B Hou, SB Geib, SM AF Calla, Bernarda Hall, Brian Hou, Shaobin Geib, Scott M. TI A genomic perspective to assessing quality of mass-reared SIT flies used in Mediterranean fruit fly (Ceratitis capitata) eradication in California SO BMC GENOMICS LA English DT Article DE Medfly; Ceratitis capitata; RNA-seq; Sterile insect technique; Irradiation; Sterilization ID GENE-EXPRESSION DATA; LIFE-HISTORY TRAITS; RNA-SEQ DATA; INBREEDING DEPRESSION; CELL-ADHESION; TEPHRITIDAE; DIPTERA; DNA; POPULATIONS; STERILE AB Background: Temperature sensitive lethal (tsl) mutants of the tephritid C. capitata are used extensively in control programs involving sterile insect technique in California. These flies are artificially reared and treated with ionizing radiation to render males sterile for further release en masse into the field to compete with wild males and disrupt establishment of invasive populations. Recent research suggests establishment of C. capitata in California, despite the fact that over 250 million sterile flies are released weekly as part of the state's preventative program. In this project, genome-level quality assessment was performed, measured as expression differences between the Vienna-7 tsl mutants used in SIT programs and wild flies. RNA-seq was performed to provide a genome-wide map of the messenger RNA populations in C. capitata, and to investigate significant expression changes in Vienna-7 mass reared flies. Results: Flies from the Vienna-7 colony showed a markedly reduced abundance of transcripts related to visual and chemical responses, including light stimuli, neural development and signaling pathways when compared to wild flies. In addition, genes associated with muscle development and locomotion were shown to be reduced. This suggests that the Vienna-7 line may be less competitive in mating and host plant finding where these stimuli are utilized. Irradiated flies showed several transcripts representing stress associated with irradiation. Conclusions: There are significant changes at the transcriptome level that likely alter the competitiveness of mass reared flies and provide justification for pursuing methods for strain improvement, increasing competitiveness of mass-reared flies, or exploring alternative SIT approaches to increase the efficiency of eradication programs. C1 [Calla, Bernarda; Geib, Scott M.] ARS, Trop Crop & Commod Protect Res Unit, USDA, Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Hall, Brian] Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Dept Plant & Environm Protect Sci, Honolulu, HI 96822 USA. [Hou, Shaobin] Univ Hawaii Manoa, Honolulu, HI 96822 USA. RP Geib, SM (reprint author), ARS, Trop Crop & Commod Protect Res Unit, USDA, Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. EM scott.geib@ars.usda.gov OI Geib, Scott/0000-0002-9511-5139; Hou, Shaobin/0000-0003-3467-8242 FU United States Department of Agriculture, Agricultural Research Service (USDA-ARS) FX We thank Steven Tam for laboratory assistance; Song Min So at the CDFA Waimanalo medfly mass rearing facility for providing access to irradiated and non-irradiated Vienna line medfly; Kauai Coffee for providing infested coffee cherry for wild medfly collection; Yale Center for Genome Analysis for Illumina HiSeq services. This work was funded by the United States Department of Agriculture, Agricultural Research Service (USDA-ARS). Opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA. USDA is an equal opportunity provider and employer. NR 37 TC 4 Z9 4 U1 1 U2 31 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 5 PY 2014 VL 15 AR 98 DI 10.1186/1471-2164-15-98 PG 19 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AA5DX UT WOS:000331117300001 PM 24495485 ER PT J AU DeVault, TL Blackwell, BF Seamans, TW Lima, SL Fernandez-Juricic, E AF DeVault, Travis L. Blackwell, Bradley F. Seamans, Thomas W. Lima, Steven L. Fernandez-Juricic, Esteban TI Effects of Vehicle Speed on Flight Initiation by Turkey Vultures: Implications for Bird-Vehicle Collisions SO PLOS ONE LA English DT Article ID MITIGATION MEASURES; AVOIDANCE-BEHAVIOR; STARTING DISTANCE; HUMAN DISTURBANCE; ECOLOGY APPROACH; ROAD MORTALITY; HOME RANGES; AIRCRAFT; RISK; BLACK AB The avoidance of motorized vehicles is a common challenge for birds in the modern world. Birds appear to rely on antipredator behaviors to avoid vehicles, but modern vehicles (automobiles and aircraft) are faster than natural predators. Thus, birds may be relatively ill-equipped, in terms of sensory capabilities and behaviors, to avoid vehicles. We examined the idea that birds may be unable to accurately assess particularly high speeds of approaching vehicles, which could contribute to miscalculations in avoidance behaviors and ultimately cause collisions. We baited turkey vultures (Cathartes aura) to roads with animal carcasses and measured flight initiation distance and effective time-to-collision in response to a truck driving directly towards vultures from a starting distance of 1.13 km and at one of three speeds: 30, 60, or 90 kph (no vultures were struck). Flight initiation distance of vultures increased by a factor of 1.85 as speed increased from 30 to 90 kph. However, for 90-kph approaches there was no clear trend in flight initiation distance across replicates: birds appeared equally likely to initiate escape behavior at 40 m as at 220 m. Time-to-collision decreased by a factor of 0.62 with approach speeds from 30 to 90 kph. Also, at 90 kph, four vehicle approaches (17%) resulted in near collisions with vultures (time-to-collision <= 1.7 s), compared to none during 60 kph approaches and one during 30 kph approaches (4%). Our findings suggest that antipredator behaviors in turkey vultures, particularly stimulus processing and response, might not be well tuned to vehicles approaching at speeds >= 90 kph. The possible inability of turkey vultures to react appropriately to high-speed vehicles could be common among birds, and might represent an important determinant of bird-vehicle collisions. C1 [DeVault, Travis L.; Blackwell, Bradley F.; Seamans, Thomas W.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. [Lima, Steven L.] Indiana State Univ, Dept Biol, Terre Haute, IN 47809 USA. [Fernandez-Juricic, Esteban] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. RP DeVault, TL (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. EM Travis.L.DeVault@aphis.usda.gov FU U.S. Department of Agriculture FX Funding was provided by the U.S. Department of Agriculture. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of this manuscript. NR 56 TC 11 Z9 11 U1 2 U2 39 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 4 PY 2014 VL 9 IS 2 AR e87944 DI 10.1371/journal.pone.0087944 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI6GY UT WOS:000336971300020 PM 24503622 ER PT J AU Allen, DK Goldford, J Gierse, JK Mandy, D Diepenbrock, C Libourel, IGL AF Allen, Doug K. Goldford, Joshua Gierse, James K. Mandy, Dominic Diepenbrock, Christine Libourel, Igor G. L. TI Quantification of Peptide m/z Distributions from C-13-Labeled Cultures with High-Resolution Mass Spectrometry SO ANALYTICAL CHEMISTRY LA English DT Article ID METABOLIC FLUX ANALYSIS; ISOTOPIC ABUNDANCE MEASUREMENTS; AMINO-ACIDS; CELL-CULTURE; PROTEOMICS; ACCURACY; C-13; PROTEINS; SYSTEMS; DESIGN AB Isotopic labeling studies of primary metabolism frequently utilize GC/MS to quantify C-13 in protein-hydrolyzed amino acids. During processing some amino acids are degraded, which reduces the size of the measurement set. The advent of high-resolution mass spectrometers provides a tool to assess molecular masses of peptides with great precision and accuracy and computationally infer information about labeling in amino acids. Amino acids that are isotopically labeled during metabolism result in labeled peptides that contain spatial and temporal information that is associated with the biosynthetic origin of the protein. The quantification of isotopic labeling in peptides can therefore provide an assessment of amino acid metabolism that is specific to subcellular, cellular, or temporal conditions. A high-resolution orbital trap was used to quantify isotope labeling in peptides that were obtained from unlabeled and isotopically labeled soybean embryos and Escherichia coli cultures. Standard deviations were determined by estimating the multinomial variance associated with each element of the m/z distribution. Using the estimated variance, quantification of the m/z distribution across multiple scans was achieved by a nonlinear fitting approach. Observed m/z distributions of uniformly labeled E. coli peptides indicated no significant differences between observed and simulated m/z distributions. Alternatively, amino acid m/z distributions obtained from GC/MS were convolved to simulate peptide m/z distributions but resulted in distinct profiles due to the production of protein prior to isotopic labeling. The results indicate that peptide mass isotopologue measurements faithfully represent mass distributions, are suitable for quantification of isotope-labeling-based studies, and provide additional information over existing methods. C1 [Allen, Doug K.; Gierse, James K.; Diepenbrock, Christine] ARS, Plant Genet Res Unit, USDA, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Goldford, Joshua; Mandy, Dominic; Libourel, Igor G. L.] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA. RP Allen, DK (reprint author), ARS, Plant Genet Res Unit, USDA, Donald Danforth Plant Sci Ctr, 975 North Warson Rd, St Louis, MO 63132 USA. EM Doug.Allen@ars.usda.gov; Libourel@umn.edu RI Allen, Doug/M-2836-2013 OI Allen, Doug/0000-0001-8599-8946 FU National Science Foundation (NSF) [NSF/MCB-1042335, EF-1105249]; Office of Naval Research (ONR) [N000141310552] FX We acknowledge resources provided by the USDA-ARS and the Donald Danforth Plant Science Center Proteomics and Mass Spectrometry Facility as well as the Minnesota Supercomputing Institute and funding support from the National Science Foundation (NSF) (Grant NSF/MCB-1042335, EF-1105249), and Office of Naval Research (ONR) (Grant N000141310552). The authors gratefully acknowledge the technical advice of Dr. Alexander Makarov, Thermo Fisher Scientific, and thank him for communicating a relationship approximating ion counts from signal to noise readings. Dr. Andre d'Avignon (High Resolution NMR Facility, Department of Chemistry, Washington University in St Louis) is acknowledged for help with NMR analysis. Any product or trademark mentioned here does not imply a warranty, guarantee, or endorsement by the authors or their affiliations over other suitable products. NR 56 TC 8 Z9 8 U1 0 U2 22 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 EI 1520-6882 J9 ANAL CHEM JI Anal. Chem. PD FEB 4 PY 2014 VL 86 IS 3 BP 1894 EP 1901 DI 10.1021/ac403985w PG 8 WC Chemistry, Analytical SC Chemistry GA AA3TI UT WOS:000331014800079 PM 24387081 ER PT J AU Oh, MH Wang, XJ Kim, SY Wu, X Clouse, SD Huber, SC AF Oh, Man-Ho Wang, Xuejun Kim, Sang Yeol Wu, Xia Clouse, Steven D. Huber, Steven C. TI The Carboxy-terminus of BAK1 regulates kinase activity and is required for normal growth of Arabidopsis (Retracted article. See vol. 7, 960, 2016) SO FRONTIERS IN PLANT SCIENCE LA English DT Article; Retracted Publication DE brassinosteroid; RAKI; BRI1; domain; phosphotyrosine; protein peptide interaction ID RECEPTOR-LIKE KINASES; PROTEIN-KINASE; PHOSPHORYLATION SITES; IN-VITRO; BRI1; ACTIVATION; DEFENSE; BRASSINOSTEROIDS; IDENTIFICATION; PERCEPTION AB Binding of brassinolide to the brassinosteroid-insenstive 1(BRI1) receptor kinase promotes interaction with its co-receptor, BRI1-associated receptor kinase 1 (BAK1). Juxtaposition of the kinase domains that occurs then allows reciprocal transphosphorylation and activation of both kinases, but details of that process are not entirely clear. In the present study we show that the carboxy (C)-terminal polypeptide of BAK1 may play a role. First, we demonstrate that the C-terminal domain is a strong inhibitor of the transphosphorylation activity of the recombinant BAK1 cytoplasmic domain protein. However, recombinant BAK1 lacking the C-terminal domain is unable to transactivate the peptide kinase activity of BRI1 in vitro. Thus, the C-terminal domain may play both a positive and negative role. Interestingly, a synthetic peptide corresponding to the full C-terminal domain (residues 576-615 of BAK1) interacted with recombinant BRI1 in vitro, and that interaction was enhanced by phosphorylation at the Tyr-610 site. Expression of a BAK1 C-terminal domain truncation (designated BAK1-Delta CT-Flag) in transgenic Arabidopsis plants lacking endogenous bak1 and its functional paralog, bkk1, produced plants that were wild type in appearance but much smaller than plants expressing full-length BAK1-Flag. The reduction in growth may be attributed to a partial inhibition of BR signaling in vivo as reflected in root growth assays but other factors are likely involved as well. Our working model is that in vivo, the inhibitory action of the C-terminal domain of BAK1 is relieved by binding to BRI1. However, that interaction is not essential for BR signaling, but other aspects of cellular signaling are impacted when the C-terminal domain is truncated and result in inhibition of growth. These results increase the molecular understanding of the C-terminal domain of BAK1 as a regulator of kinase activity that may serve as a model for other receptor kinases. C1 [Oh, Man-Ho; Wang, Xuejun; Kim, Sang Yeol; Wu, Xia; Huber, Steven C.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Oh, Man-Ho] Chungnam Natl Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Taejon, South Korea. [Wu, Xia] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA. [Clouse, Steven D.] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. [Huber, Steven C.] ARS, USDA, Urbana, IL USA. RP Huber, SC (reprint author), Univ Illinois, Dept Plant Biol, 1201 West Gregory Dr,197 ERML, Urbana, IL 61801 USA. EM schuber1@illinois.edu RI Wu, Xia/J-8690-2014 OI Wu, Xia/0000-0002-0024-4481 FU National Science Foundation [IOS-1022177, MCB-1021363]; US Department of Agriculture (USDA)-Agricultural Research Service (ARS) FX This work was supported in part by the National Science Foundation (IOS-1022177and MCB-1021363) and the US Department of Agriculture (USDA)-Agricultural Research Service (ARS). The authors thank Dr. Cyril Zipfel for the generous gift of BAK1-CT antibodies. NR 30 TC 10 Z9 10 U1 1 U2 19 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 4 PY 2014 VL 5 AR 16 DI 10.3389/fpls.2014.00016 PG 9 WC Plant Sciences SC Plant Sciences GA AB1EH UT WOS:000331533800001 PM 24550926 ER PT J AU Griffin, MJ Ware, C Quiniou, SM Steadman, JM Gaunt, PS Khoo, LH Soto, E AF Griffin, Matt J. Ware, Cynthia Quiniou, Sylvie M. Steadman, James M. Gaunt, Patricia S. Khoo, Lester H. Soto, Esteban TI Edwardsiella piscicida identified in the southeastern USA by gyrB sequence, species-specific and repetitive sequence-mediated PCR SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE Edwardsiella tarda; E. piscicida; gyrB; PCR; Repetitive sequence-mediated PCR; Rep-PCR ID POLYMERASE-CHAIN-REACTION; GENOME SEQUENCE; ENTERIC SEPTICEMIA; CHANNEL CATFISH; CAUSATIVE AGENT; REACTION ASSAY; TARDA STRAINS; NILE TILAPIA; E. TARDA; ICTALURI AB A new Edwardsiella taxon was recently described from fishes of Europe and Asia. Phenotypically similar to E. tarda, extensive genetic and phenotypic characterization determined this new strain does not belong to any established Edwardsiella taxa, leading to the adoption of a new taxon, E. piscicida. Concurrent research in the USA also identified 2 genetically distinct taxa within the group of organisms traditionally classified as E. tarda. Comparisons of gyrB sequences between US isolates and E. piscicida from Europe and Asia identified several US isolates with > 99.6% similarity to the gyrB sequence of the E. piscicida type strain (ET883) but < 87% similarity to the E. tarda type strain (ATCC #15947). A discriminatory PCR was developed for the identification of E. tarda and 2 genetic variants of E. piscicida (E. piscicida and E. piscicida-like species). Using these PCR assays, a survey was conducted of 44 archived bacterial specimens from disease case submissions to the Aquatic Research and Diagnostic Laboratory (Stoneville, MS, USA) between 2007 and 2012. All 44 isolates, originally identified phenotypically and biochemically as E. tarda, were identified as E. piscicida by PCR. Repetitive sequence-mediated PCR (rep-PCR) analysis of these archived specimens suggests they are largely homogenous, similar to what has been observed for E. ictaluri. The gyrB sequence data, coupled with the E. piscicida specific-PCR and rep-PCR data, confirms that E. piscicida has been isolated from fish disease cases in the southeastern USA. Moreover, our survey data suggests E. piscicida may be more prevalent in catfish aquaculture than E. tarda. C1 [Griffin, Matt J.; Ware, Cynthia; Steadman, James M.; Gaunt, Patricia S.; Khoo, Lester H.] Mississippi State Univ, Coll Vet Med, Thad Cochran Natl Warmwater Aquaculture Ctr, Aquat Res & Diagnost Lab, Stoneville, MS 38776 USA. [Quiniou, Sylvie M.] USDA ARS, Warmwater Aquaculture Res Unit, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. [Soto, Esteban] Ross Univ, Sch Vet Med, Marine Res Lab, Dept Pathobiol, Basseterre, St Kitts, W Ind Assoc St. RP Griffin, MJ (reprint author), Mississippi State Univ, Coll Vet Med, Thad Cochran Natl Warmwater Aquaculture Ctr, Aquat Res & Diagnost Lab, POB 197,127 Expt Stn Rd, Stoneville, MS 38776 USA. EM griffin@cvm.msstate.edu FU United States Department of Agriculture Agricultural Research Service [58-6402-2729]; United States Department of Agriculture Catfish Health Research Initiative [CRIS 6402-31320-002-02]; Mississippi State University College of Veterinary Medicine; Mississippi Agricultural and Forestry Experiment Station (MAFES) FX The present manuscript is based upon work supported by the United States Department of Agriculture Agricultural Research Service (Project No. 58-6402-2729), the United States Department of Agriculture Catfish Health Research Initiative (CRIS 6402-31320-002-02), the Mississippi State University College of Veterinary Medicine, and the Mississippi Agricultural and Forestry Experiment Station (MAFES). This is MAFES publication number J-12433. NR 37 TC 8 Z9 8 U1 2 U2 9 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD FEB 4 PY 2014 VL 108 IS 1 BP 23 EP 35 DI 10.3354/dao02687 PG 13 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA 304CF UT WOS:000330722700003 PM 24492051 ER PT J AU Liu, NT Nou, XW Lefcourt, AM Shelton, DR Lo, YM AF Liu, Nancy T. Nou, Xiangwu Lefcourt, Alan M. Shelton, Daniel R. Lo, Y. Martin TI Dual-species biofilm formation by Escherichia coli O157:H7 and environmental bacteria isolated from fresh-cut processing facilities SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Biofilms; Escherichia coli O157:H7; Ralstonia insidiosa; Environmental bacteria ID SURVIVAL; O157-H7; STRAINS; LETTUCE; SALMONELLA; RESISTANCE; OUTBREAKS; O157/H7; ACID AB Biofilm formation is a mechanism adapted by many microorganisms that enhances the survival in stressful environments. In food processing facilities, foodborne bacterial pathogens, which many are poor biofilm formers, could potentially take advantage of this protective mechanism by interacting with other strong biofilm producers. The objective of this study was to determine the influence of bacteria native to fresh produce processing environments on the incorporation of Escherichia coli O157:H7 in biofilms. Bacteria strains representing 13 Gram-negative species isolated from two fresh produce processing facilities in a previous study were tested for forming dual-species biofilms with E. coli O157:H7. Strong biofilm producing strains of Burkholderia caryophylli and Ralstonia insidiosa exhibited 180% and 63% increase in biofilm biomass, and significant thickening of the biofilms (B. caryophylli not tested), when co-cultured with E. coli O157:H7. E. coli O157:H7 populations increased by approximately 1 log in dual-species biofilms formed with B. caryophylli or R. insidiosa. While only a subset of environmental isolates with strong biofilm formation abilities increased the presence of E. coli O157:H7 in biofilms, all tested E. coli O157:H7 exhibited higher incorporation in dual-species biofilms with R. insidiosa. These observations support the notion that E. coli O157:H7 and specific strong biofilm producing bacteria interact synergistically in biofilm formation, and suggest a route for increased survival potential of E. coli O157:H7 in fresh produce processing environments. Published by Elsevier B.V. C1 [Liu, Nancy T.; Nou, Xiangwu; Lefcourt, Alan M.; Shelton, Daniel R.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Liu, Nancy T.; Lo, Y. Martin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20740 USA. RP Nou, XW (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM xiangwu.nou@ars.usda.gov FU Chinese Scholarship Council, Beijing, China FX We thank Drs. R. Mandrell and M. Mammel for providing some of the E. coli O157:H7 strains used in this study, and Dr. R. Buchanan for critically reading the manuscript and making suggestions for revision. NTL was partly supported by a scholarship from Chinese Scholarship Council, Beijing, China. NR 24 TC 16 Z9 16 U1 0 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD FEB 3 PY 2014 VL 171 BP 15 EP 20 DI 10.1016/j.ijfoodmicro.2013.11.007 PG 6 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA AA8LV UT WOS:000331347900003 PM 24296258 ER PT J AU Kingsley, DH Vincent, EM Meade, GK Watson, CL Fan, XT AF Kingsley, David H. Vincent, Emily M. Meade, Gloria K. Watson, Clytrice L. Fan, Xuetong TI Inactivation of human norovirus using chemical sanitizers SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Human norovirus; Chlorine; Peroxyacetic acid; Trisodium phosphate; Chlorine dioxide; Hydrogen peroxide ID BLOOD GROUP ANTIGEN; PORCINE GASTRIC MUCIN; FELINE CALICIVIRUS; MURINE NOROVIRUS; HYDROGEN-PEROXIDE; CHLORINE DIOXIDE; DISINFECTION KINETICS; GII.4 NOROVIRUS; ENTERIC VIRUSES; NORWALK VIRUS AB The porcine gastric mucin binding magnetic bead (PGM-MB) assay was used to evaluate the ability of chlorine, chlorine dioxide, peroxyacetic acid, hydrogen peroxide, and trisodium phosphate to inactivate human norovirus within 10% stool filtrate. One-minute free chlorine treatments at concentrations of 33 and 189 ppm reduced virus binding in the PGM-MB assay by 1.48 and 4.14 log(10), respectively, suggesting that chlorine is an efficient sanitizer for inactivation of human norovirus (HuNoV). Five minute treatments with 5% trisodium phosphate (pH similar to 12) reduced HuNoV binding by 1.6 log(10), suggesting that TSP, or some other high pH buffer, could be used to treat food and food contact surfaces to reduce HuNoV. One minute treatments with 350 ppm chlorine dioxide dissolved in water did not reduce PGM-MB binding, suggesting that the sanitizer may not be suitable for HuNoV inactivation in liquid form. However a 60-min treatment with 350 ppm chlorine dioxide did reduce human norovirus by 2.8 log(10), indicating that chlorine dioxide had some, albeit limited, activity against HuNoV. Results also suggest that peroxyacetic acid has limited effectiveness against human norovirus, since 1-min treatments with up to 195 ppm reduced human norovirus binding by <1 log(10). Hydrogen peroxide (4%) treatment of up to 60 min resulted in minimal binding reduction (similar to 0.1 log(10)) suggesting that H2O2 is not a good liquid sanitizer for HuNoV. Overall this study suggests that HuNoV is remarkably resistant to several commonly used disinfectants and advocates for the use of chlorine (sodium hypochlorite) as a HuNoV disinfectant wherever possible. Published by Elsevier B.V. C1 [Kingsley, David H.; Meade, Gloria K.] Delaware State Univ, Food Safety & Intervent Technol Res Unit, USDA, Agr Res Serv, Dover, DE 19901 USA. [Vincent, Emily M.; Watson, Clytrice L.] Delaware State Univ, Dept Biol Sci, Dover, DE 19901 USA. [Fan, Xuetong] Agr Res Serv, USDA, Residue Chem & Predict Microbiol Res Unit, Wyndmoor, PA 19038 USA. RP Kingsley, DH (reprint author), Delaware State Univ, Food Safety & Intervent Technol Res Unit, USDA, Agr Res Serv, Dover, DE 19901 USA. EM david.kingsley@ars.usda.gov NR 52 TC 18 Z9 18 U1 1 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD FEB 3 PY 2014 VL 171 BP 94 EP 99 DI 10.1016/j.ijfoodmicro.2013.11.018 PG 6 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA AA8LV UT WOS:000331347900013 PM 24334094 ER PT J AU Huang, LH AF Huang, Lihan TI IPMP 2013-A comprehensive data analysis tool for predictive microbiology SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Predictive microbiology; Model development; Nonlinear regression; Software development ID LISTERIA-MONOCYTOGENES; GROWTH RATE; BACTERIAL-GROWTH; FOOD MICROBIOLOGY; SODIUM-CHLORIDE; TEMPERATURE; MODEL; KINETICS; BEEF; INACTIVATION AB Predictive microbiology is an area of applied research in food science that uses mathematical models to predict the changes in the population of pathogenic or spoilage microorganisms in foods exposed to complex environmental changes during processing, transportation, distribution, and storage. It finds applications in shelf-life prediction and risk assessments of foods. The objective of this research was to describe the performance of a new user-friendly comprehensive data analysis tool, the Integrated Pathogen Modeling Model (IPMP 2013), recently developed by the USDA Agricultural Research Service. This tool allows users, without detailed programming knowledge, to analyze experimental kinetic data and fit the data to known mathematical models commonly used in predictive microbiology. Data curves previously published in literature were used to test the models in IPMP 2013. The accuracies of the data analysis and models derived from IPMP 2013 were compared in parallel to commercial or open-source statistical packages, such as SAS (R) or R. Several models were analyzed and compared, including a three-parameter logistic model for growth curves without lag phases, reduced Huang and Baranyi models for growth curves without stationary phases, growth models for complete growth curves (Huang, Baranyi, and re-parameterized Gompertz models), survival models (linear, re-parameterized Gompertz, and Weibull models), and secondary models (Ratkowsky square-root, Huang square-root, Cardinal, and Arrhenius-type models). The comparative analysis suggests that the results from IPMP 2013 were equivalent to those obtained from SAS (R) or R. This work suggested that the IPMP 2013 could be used as a free alternative to SAS (R), R, or other more sophisticated statistical packages for model development in predictive microbiology. Published by Elsevier B.V. C1 [Huang, Lihan] USDA ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Huang, LH (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov NR 24 TC 20 Z9 23 U1 7 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD FEB 3 PY 2014 VL 171 BP 100 EP 107 DI 10.1016/j.ijfoodmicro.2013.11.019 PG 8 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA AA8LV UT WOS:000331347900014 PM 24334095 ER PT J AU Chu, ML Guzman, JA Munoz-Carpena, R Riker, GA Linkov, I AF Chu, M. L. Guzman, J. A. Munoz-Carpena, R. Riker, G. A. Linkov, I. TI A simplified approach for simulating changes in beach habitat due to the combined effects of long-term sea level rise, storm erosion, and nourishment SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE SLAMM; Coastal erosion; Beach nourishment; Hurricanes; Risk contours ID DUNE EROSION; IMPACTS; FLORIDA; ACCRETION; ISLAND; RATES; COAST AB Better understanding of the vulnerability of coastal habitats to sea level rise and major storm events are aided by the use of simulation models. Since coastal habitats also undergo frequent nourishment restoration works in order to maintain their viability, vulnerability models must be able to assess the combined effects of sea level rise, storm surge, and beach nourishment. The Sea Level Affecting Marshes Model (SLAMM) was modified and applied to quantify the changes in the beach area in a 5-km stretch of beach in Santa Rosa Island, Florida due to these combined effects. A new methodology to estimate spatial erosion patterns was developed based on measured erosion during three historic storm events representing a wide range of storm intensities over the study area (named storms Ivan (H5), Dennis (H4), and Katrina (TS)). Future major storms over the 2012-2100 period were generated based on the frequency distribution of historic storms using 4000 simulations to account for uncertainty in the storms temporal distribution. Potential effects of individual, successive, and random storms occurring over the area under 0-1.5 m nourishment schemes were evaluated. The risk of losing the beach habitat in 90 years for different scenarios is studied based on probability distribution contours constructed with the model results. Simulation results suggest that without nourishment, a major storm with a category of tropical storm or higher will reduce the beach at the end of the period by 97-100%. This loss can be reduced to 60% by maintaining a 1-m beach elevation and can further be reduced to 34% with 1.5 m beach nourishment. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Chu, M. L.; Munoz-Carpena, R.; Riker, G. A.] Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA. [Guzman, J. A.] USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. [Linkov, I.] US Army, Engn Res & Dev Ctr, Concord, MA 01366 USA. [Riker, G. A.] Univ KwaZulu Natal, SMCS, Durban, South Africa. RP Munoz-Carpena, R (reprint author), Univ Florida, Agr & Biol Engn Dept, POB 110570, Gainesville, FL 32611 USA. EM carpena@ufl.edu OI GUZMAN, Jorge A/0000-0002-7734-5723; Munoz-Carpena, Rafael/0000-0003-2838-1514 FU Department of Defense Strategic Environmental Research and Development Program [SI-1699]; UF Research Foundation Professorship; UF Water Institute Faculty Fellowship; University of Florida, High-Performance Computing Center FX Funding was provided by the Department of Defense Strategic Environmental Research and Development Program (project SI-1699). Permission was granted by the USACE Chief of Engineers to publish this material. The views and opinions expressed in this paper are those of the individual authors and not those of the U.S. Army, or other sponsor organizations. The authors acknowledge the University of Florida, High-Performance Computing Center (http://hpc.ufl.edu) for providing computational resources and support that have contributed to the results reported in this paper. Dr. Carpena acknowledges support from the UF Research Foundation Professorship and the UF Water Institute Faculty Fellowship. NR 37 TC 1 Z9 1 U1 8 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD FEB PY 2014 VL 52 BP 111 EP 120 DI 10.1016/j.envsoft.2013.10.020 PG 10 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA AX2CY UT WOS:000346751700010 ER PT J AU Jaradat, AA AF Jaradat, Abdullah A. TI The Value of Wheat Landraces SO EMIRATES JOURNAL OF FOOD AND AGRICULTURE LA English DT Editorial Material C1 [Jaradat, Abdullah A.] Univ Minnesota, ARS, USDA, Morris, MN 56267 USA. [Jaradat, Abdullah A.] Univ Minnesota, Dept Agron & Plant Genet, Morris, MN 56267 USA. RP Jaradat, AA (reprint author), Univ Minnesota, ARS, USDA, 803 Iowa Ave, Morris, MN 56267 USA. EM abdullah.jaradat@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU UNITED ARAB EMIRATES UNIV PI AL AIN PA P. O. BOX 17551, AL AIN, U ARAB EMIRATES SN 2079-052X EI 2079-0538 J9 EMIR J FOOD AGR JI Emir. J. Food Agric. PD FEB PY 2014 VL 26 IS 2 SI SI BP 92 EP 92 DI 10.9755/ejfa.v26i2.16745 PG 1 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA AN8MH UT WOS:000340857800001 ER PT J AU Jaradat, AA Shahid, M AF Jaradat, Abdullah A. Shahid, Mohammad TI How diverse a farmer-managed wheat landrace can be? SO EMIRATES JOURNAL OF FOOD AND AGRICULTURE LA English DT Article DE Genetic Diversity; Landrace; Oman; Phenotyping; Wheat ID GENETIC DIVERSITY; DURUM-WHEAT; PHENOTYPIC DIVERSITY; YIELD COMPONENTS; DROUGHT STRESS; BREAD WHEAT; OMANI WHEAT; TRITICUM; TRAITS; CULTIVARS AB Phenotypic variation in phenological, quantitative and qualitative traits was assessed in geographically-isolated, farmer-managed wheat landrace populations grown under subsistence farming conditions. Several multivariate, genetic diversity and structural equation modeling procedures were used to build a comprehensive structure of the landrace and to (1) identify and construct multivariate distances between components of the landrace, (2) identify plant- and seed-related traits contributing to its composition, (3) build principal components that can account for maximum variation, (4) quantify variance components accounted for by major seed qualitative traits, (5) partition total diversity and estimate levels of population differentiation, (6) build and validate a predictive model of landrace population-trait association, (7) identify traits affecting spikelet fertility as a critical component of grain yield under the prevailing hot conditions in Oman, and (8) construct and interpret structural equation models to estimate the direct and indirect effects of quantitative and qualitative traits on grain yield per plant for each landrace population. The results will be discussed within the context of on-farm conservation and sustainable utilization of endangered wheat landrace populations under subsistence farming and to illustrate the use of advanced multivariate statistical methods in assessing phenotypic variation in subdivided landrace populations. C1 [Jaradat, Abdullah A.] Univ Minnesota, ARS, USDA, Morris, MN 56267 USA. [Jaradat, Abdullah A.] Univ Minnesota, Dept Agron & Plant Genet, Morris, MN 56267 USA. [Shahid, Mohammad] ICBA, Plant Genet Resources Program, Dubai, U Arab Emirates. RP Jaradat, AA (reprint author), Univ Minnesota, ARS, USDA, 803 Iowa Ave, Morris, MN 56267 USA. EM Abdullah.Jaradat@ars.usda.gov FU ICBA, Dubai, United Arab Emirates; USDA-ARS project,Morris, MN, USA [3645-61600-001-00D] FX We would like to thank the field staff who contributed to this research. This research was supported by ICBA, Dubai, United Arab Emirates,and by USA-ARS project No. 3645-61600-001-00D, Morris, MN, USA. The use off trade, firm, or corporation names in this publication is for information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. The USDA is an equal provider and employer. NR 52 TC 2 Z9 2 U1 1 U2 2 PU UNITED ARAB EMIRATES UNIV PI AL AIN PA P. O. BOX 17551, AL AIN, U ARAB EMIRATES SN 2079-052X EI 2079-0538 J9 EMIR J FOOD AGR JI Emir. J. Food Agric. PD FEB PY 2014 VL 26 IS 2 SI SI BP 93 EP 118 DI 10.9755/ejfa.v26i2.16753 PG 26 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA AN8MH UT WOS:000340857800002 ER PT J AU Jaradat, AA AF Jaradat, Abdullah A. TI The vanishing wheat landraces of the Fertile Crescent SO EMIRATES JOURNAL OF FOOD AND AGRICULTURE LA English DT Review DE Climate change; Diversity; Fertile Crescent; Genetic erosion; Landrace ID YIELD FORMATION STRATEGIES; IN-SITU CONSERVATION; DURUM DESF. MACKEY; MEDITERRANEAN BASIN; GENETIC-RESOURCES; GRAIN-YIELD; CEREAL LANDRACES; DISTINCT PATTERN; SEED MANAGEMENT; DIVERSITY AB Genetic diversity of wheat landraces constituted a sizable portion of the mega diversity in the Fertile Crescent as a center of origin and of diversity of major crop plants. Following wheat domestication in the Fertile Crescent, early farmers developed diverse wheat landraces, and contributed to their evolution, enrichment, on-farm conservation and developed efficient seed exchange systems, and ensured the continued evolution and diversification of these landraces. For millennia, wheat landraces served as staples for food production in the Fertile Crescent and beyond; however, their use dramatically declined in the 2nd half of the last century and they were replaced by modern high-yielding cultivars. Wheat landraces are facing the risk of genetic pollution, and genetic erosion and extinction due to anthropogenic and natural causes, including climate change. Monitoring and reversing genetic erosion in wheat landraces are scientifically challenging, technically and logistically difficult, and require long-term dedication and extensive efforts of farmers and the public sector. This paper presents in-depth assessment of the status of the 'vanishing' wheat landraces of the Fertile Crescent, their vulnerability to predicted climate change, and proposes a strategy to give evolution a second chance to engage in reversing this trend and restoring the allelic richness of these landraces. C1 [Jaradat, Abdullah A.] Univ Minnesota, ARS, USDA, Morris, MN 56267 USA. [Jaradat, Abdullah A.] Univ Minnesota, Dept Agron & Plant Genet, Morris, MN 56267 USA. RP Jaradat, AA (reprint author), Univ Minnesota, ARS, USDA, 803 Iowa Ave, Morris, MN 56267 USA. EM Abdullah.Jaradat@ars.usda.gov FU USDA-ARS, Morris, MN [3645-61600-001*00D] FX I would like to thank Dr. I. Rawashdeh for contributing images of seed, spike and plants of the durum wheat landrace Noorseh from Jordan. This research was funded by USDA-ARS Project No. 3645-61600-001*00D, Morris, MN. The use of trade, firm, or corporation names in this publication is for information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service for any product or service to the exclusion of others that may be suitable. USDA is an equal provider and employer. NR 59 TC 2 Z9 2 U1 0 U2 9 PU UNITED ARAB EMIRATES UNIV PI AL AIN PA P. O. BOX 17551, AL AIN, U ARAB EMIRATES SN 2079-052X EI 2079-0538 J9 EMIR J FOOD AGR JI Emir. J. Food Agric. PD FEB PY 2014 VL 26 IS 2 SI SI BP 203 EP 217 DI 10.9755/ejfa.v26i2.17542 PG 15 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA AN8MH UT WOS:000340857800011 ER PT J AU Peterson, SC Jackson, MA AF Peterson, Steven C. Jackson, Michael A. TI Simplifying pyrolysis: Using gasification to produce corn stover and wheat straw biochar for sorptive and horticultural media SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biochar; Pyrolysis; Gasification; Corn stover; Wheat straw ID CARBON SEQUESTRATION; SOIL; BIOMASS; BIOAVAILABILITY; FTIR; WOOD; SPECTROSCOPY; FEEDSTOCKS; FRACTIONS; SYSTEMS AB Biochar is a renewable, useful material that can be utilized in many different applications. Biochar is commonly produced via pyrolysis methods using a retort-style oven with inert gas. Gasification is another method that can utilize pyrolysis to produce biochar. This method has significant processing advantages; it can be carried out in normal atmospheric conditions, eliminating the use of inert gas, is more amenable to scale. up, and provides heat that can be used to generate power and/or help recoup processing costs. In this work, a simple gasification process using a top-lit updraft style cookstove was used to produce biochars from corn stover, wheat straw, and wheat straw treated with glycerin, which were then compared to biochars made using the more conventional retort oven pyrolysis process. The glycerin-treated wheat straw biochar from the gasification process had carbon content > 70% and an ash content of 25% which was equal or better than the same biochar produced using the retort oven. This biochar has also shown successful use as a peat moss replacement in horticultural applications. This shows that gasification is a simpler, more cost-effective means to produce biochars and should be considered for horticultural and other similar applications. Published by Elsevier B.V. C1 [Peterson, Steven C.; Jackson, Michael A.] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Peterson, SC (reprint author), USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Steve.Peterson@ars.usda.gov NR 41 TC 8 Z9 8 U1 9 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD FEB PY 2014 VL 53 BP 228 EP 235 DI 10.1016/j.indcrop.2013.12.028 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA AK7MN UT WOS:000338612500029 ER PT J AU Cusimano, JSP Hart, MM Cermak, DM Cermak, SC Durham, AL AF Cusimano, John S. P. Hart, Margaret M. Cermak, Diana M. Cermak, Steven C. Durham, Amber L. TI Synthesis of lesquerella alpha-hydroxy phosphonates SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Lesquerella oil; Lesquerolic acid; alpha-Hydroxy phosphonates ID CASTOR-OIL; PHYSICAL-PROPERTIES; ESTERS; ACIDS; OXIDATION; LUBRICITY; BIODIESEL; ESTOLIDES; SEEDS AB Hydroxy fatty acids (HFAs) have found a number of uses in today's market, with uses ranging from industrial materials to pharmaceuticals. Castor oil, which is obtained from castor seeds, has served as a source of a versatile HFA; its principle component, ricinoleic acid, can be isolated from castor oil and has been modified extensively for a number of applications. Unfortunately, castor seeds also contain several undesirable compounds which pose severe health risks, including ricin, an unusually stable, deadly protein; ricinine, a poisonous alkaloid; and several allergens. Lesquerella oil, obtained from seeds of the Lesquerella fendleri species, has been identified as the most promising alternative source of HFAs. Lesquerolic acid, the primary HFA found in lesquerella oil, is also most homologous to ricinoleic acid; the only structural difference between the two is that lesquerolic acid possesses two additional methylene groups on the carboxyl end of the molecule. This structural and chemical similarity further suggests lesquerella oil and its derivatives may function as a competitive alternative to castor oil. Unfortunately, the desired HFA, lesquerolic acid, constitutes approximately 50% of lesquerella oil where castor oil contains approximately 90% of the desired HFA. This difference in desired HFA abundance in lesquerella oil required more extensive purification measures and careful identification of the correct component. Additionally, alpha-hydroxy phosphonates and their corresponding phosphonic acids are functional moieties that have been shown to display a wide variety of biological activities, as enzyme inhibitors, pesticides, antibiotics and anti-cancer therapeutics. We previously reported the synthesis of two families of alpha-hydroxy phosphonic acids based on ricinoleic acid: a family that retains the cis alkene found in ricinoleic acid and one where the alkene has undergone hydrogenation to produce a saturated alpha-hydroxy phosphonic acid. We now report the purification of the desired lesquerolic acid component of lesquerella oil and synthesis of lesquerolic acid-derived alpha-hydroxy phosphonates, both the unsaturated and saturated families. As with the ricinoleic acid-based compounds, the lesquerolic acid-based compounds have been produced in high yields and high purity, and the synthesis of these compounds is reported in this manuscript. (C) 2013 Elsevier B.V. All rights reserved. C1 [Cusimano, John S. P.; Hart, Margaret M.; Cermak, Diana M.] Knox Coll, Dept Chem, Galesburg, IL 61401 USA. [Cermak, Steven C.; Durham, Amber L.] USDA ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Cermak, DM (reprint author), Knox Coll, Dept Chem, 2 E South St, Galesburg, IL 61401 USA. EM dcermak@knox.edu; steven.cermak@ars.usda.gov FU Knox College Richter Memorial Scholars Program; Knox College Baker-Velde Scholarship Program FX The authors are grateful to Dr. Karl Vermillion (ARS-NCAUR) for his help with the 500 MHz NMR spectra. This research was supported by the Knox College Richter Memorial Scholars Program and the Knox College Baker-Velde Scholarship Program. NR 29 TC 3 Z9 3 U1 1 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD FEB PY 2014 VL 53 BP 236 EP 243 DI 10.1016/j.indcrop.2013.12.031 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA AK7MN UT WOS:000338612500030 ER PT J AU Cane, JH Dunne, R AF Cane, James H. Dunne, Rick TI Generalist Bees Pollinate Red-flowered Penstemon eatonii: Duality in the Hummingbird Pollination Syndrome SO AMERICAN MIDLAND NATURALIST LA English DT Article ID PSEUDOSPECTABILIS AB The red tubular flowers of Penstemon eatonii (Plantaginaceae) typify the classic pollination syndrome for hummingbirds. Bees are thought to diminish its seed siring potential, but we found that foraging female generalist bees (Apis, Anthophora) deposited substantial amounts of conspecific pollen on P. eatonii stigmas. In the absence of hummingbirds, bee pollination of cultivated P. eatonii annually generated massive seed yields from a 1.5 ha field. Most penstemons with red tubular flowers like P. eatonii present a symmetrically flared floral opening that facilitates landing by foraging bees. Derived floral traits that attract and position nectar-foraging hummingbirds for efficient pollen export (red deeply tubular flowers secreting abundant dilute nectar) need not compromise pollination and seed production that result from visitation by generalist bees seeking pollen and/or nectar. C1 [Cane, James H.] Utah State Univ, USDA, ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. [Dunne, Rick] Absaroka Farm, Worland, WY 82401 USA. RP Cane, JH (reprint author), Utah State Univ, USDA, ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. NR 22 TC 0 Z9 0 U1 5 U2 20 PU AMER MIDLAND NATURALIST PI NOTRE DAME PA UNIV NOTRE DAME, BOX 369, ROOM 295 GLSC, NOTRE DAME, IN 46556 USA SN 0003-0031 EI 1938-4238 J9 AM MIDL NAT JI Am. Midl. Nat. PD FEB PY 2014 VL 171 IS 2 BP 365 EP 370 PG 6 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AK1XG UT WOS:000338211800014 ER PT J AU Kurtzman, CP AF Kurtzman, Cletus P. TI Use of gene sequence analyses and genome comparisons for yeast systematics SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Review ID MULTIGENE PHYLOGENETIC ANALYSIS; INTERNAL TRANSCRIBED SPACER; LARGE-SUBUNIT RDNA; SP NOV.; BASIDIOMYCETOUS YEASTS; ASCOMYCETOUS YEASTS; FEEDING BEETLES; D1/D2 DOMAIN; FUNGI; EVOLUTION AB Detection, identification and classification of yeasts have undergone a major transformation in the past decade and a half following application of gene sequence analyses and genome comparisons. Development of a database (barcode) of easily determined gene sequences from domains 1 and 2 (D1/D2) of large subunit rRNA and from the internal transcribed spacer (ITS) now permits many laboratories to identify species accurately and this has led to a doubling in the number of known species of yeasts over the past decade. Phylogenetic analysis of gene sequences has resulted in major revision of yeast systematics, resulting in redefinition of nearly all genera. Future work calls for application of genomics to refine our understanding of the species concept and to provide a better understanding of the boundaries of genera and higher levels of classification. This increased understanding of phylogeny is expected to allow prediction of the genetic potential of various clades and species for biotechnological applications and adaptation to environmental changes. C1 ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Kurtzman, CP (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM cletus.kurtzman@ars.usda.gov NR 61 TC 12 Z9 13 U1 1 U2 22 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD FEB PY 2014 VL 64 BP 325 EP 332 DI 10.1099/ijs.0.054197-0 PN 2 PG 8 WC Microbiology SC Microbiology GA AJ8CR UT WOS:000337930800002 PM 24505070 ER PT J AU Ludi, AB Horton, DL Li, Y Mahapatra, M King, DP Knowles, NJ Russell, CA Paton, DJ Wood, JLN Smith, DJ Hammond, JM AF Ludi, A. B. Horton, D. L. Li, Y. Mahapatra, M. King, D. P. Knowles, N. J. Russell, C. A. Paton, D. J. Wood, J. L. N. Smith, D. J. Hammond, J. M. TI Antigenic variation of foot-and-mouth disease virus serotype A SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID GENETIC EVOLUTION; MOLECULAR CHARACTERIZATION; MAXIMUM-LIKELIHOOD; RECEPTOR; EPIDEMIOLOGY; ANTIBODIES; EUROPE; SITES AB The current measures to control foot-and-mouth disease (FMD) include vaccination, movement control and slaughter of infected or susceptible animals. One of the difficulties in controlling FMD by vaccination arises due to the substantial diversity found among the seven serotypes of FMD virus (FMDV) and the strains within these serotypes. Therefore, vaccination using a single vaccine strain may not fully cross-protect against all strains within that serotype, and therefore selection of appropriate vaccines requires serological comparison of the field virus and potential vaccine viruses using relationship coefficients (r(1) values). Limitations of this approach are that antigenic relationships among field viruses are not addressed, as comparisons are only with potential vaccine virus. Furthermore, inherent variation among vaccine sera may impair reproducibility of one-way relationship scores. Here, we used antigenic cartography to quantify and visualize the antigenic relationships among FMD serotype A viruses, aiming to improve the understanding of FMDV antigenic evolution and the scope and reliability of vaccine matching. Our results suggest that predicting antigenic difference using genetic sequence alone or by geographical location is not currently reliable. We found co-circulating lineages in one region that were genetically similar but antigenically distinct. Nevertheless, by comparing antigenic distances measured from the antigenic maps with the full capsid (P1) sequence, we identified a specific amino acid substitution associated with an antigenic mismatch among field viruses and a commonly used prototype vaccine strain, A(22)/IRO124/64. C1 [Ludi, A. B.; Li, Y.; Mahapatra, M.; King, D. P.; Knowles, N. J.; Paton, D. J.; Hammond, J. M.] Pirbright Inst, Pirbright GU24 0NF, Surrey, England. [Ludi, A. B.; Horton, D. L.; Russell, C. A.; Wood, J. L. N.] Univ Cambridge, Dept Vet Med, Dis Dynam Unit, Cambridge CB3 0ES, England. [Ludi, A. B.; Horton, D. L.; Smith, D. J.] Univ Cambridge, Dept Zool, Cambridge CB2 3EJ, England. [Horton, D. L.] Anim Hlth & Vet Labs Agcy, Addlestone KT15 3NB, Surrey, England. [Russell, C. A.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Smith, D. J.] Erasmus MC, Dept Virol, NL-3000 CA Rotterdam, Netherlands. [Russell, C. A.; Smith, D. J.] Univ Cambridge, WHO Collaborating Ctr Modelling Evolut & Control, Cambridge CB2 3EJ, England. RP Ludi, AB (reprint author), ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. EM Anna.Ludi@ars.usda.gov; drliy@yahoo.com RI Horton, Daniel/D-9909-2011; APHA, Staff publications/E-6082-2010; Wood, James/A-1626-2008; Institute, Pirbright/K-4476-2014; OI Horton, Daniel/0000-0002-9126-2756; Wood, James/0000-0002-0258-3188; King, Donald/0000-0002-6959-2708; Russell, Colin/0000-0002-2113-162X FU Cambridge Infectious Diseases Consortium of the Veterinary Training and Research Initiative (Defra grant) [VT0105]; Alborada Trust; Research and Policy for Infectious Disease Dynamics program of the Science and Technology Directorate, Department of Homeland Security and Fogarty International Center; EU [228292, 223498]; Defra UK [SE2939]; NIH Director's Pioneer Award [DP1-0D000490-0]; ANTIGONE [278976]; University Research Fellowship from the Royal Society FX Thanks are due to Bob Statham and the WRL/EURL FMD team for their advice and expertise on the VNT and r1 values. A. B. L. was supported by a fellowship from the Cambridge Infectious Diseases Consortium of the Veterinary Training and Research Initiative (Defra grant VT0105). J. L. N. W. is supported by the Alborada Trust and the Research and Policy for Infectious Disease Dynamics program of the Science and Technology Directorate, Department of Homeland Security and Fogarty International Center. D. L. H. is partially supported by the Cambridge Infectious Diseases Consortium Veterinary Training and Research Initiative (Defra grant VT0105) and EU FP7 Research Infrastructure Grant 'European Virus Archive (EVA)' (grant 228292). N. J. K. was partially supported by a Defra UK grant (SE2939). D. J. S. and C. A. R. acknowledge the support of EU FP7 grants EMPERIE (223498). C. A. R. acknowledges the support of the NIH Director's Pioneer Award DP1-0D000490-0 and D.J. S., C. A. R. and J. L. N. W. are also supported by ANTIGONE (278976). C. A. R. was supported by a University Research Fellowship from the Royal Society. NR 46 TC 6 Z9 6 U1 0 U2 10 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD FEB PY 2014 VL 95 BP 384 EP 392 DI 10.1099/vir.0.057521-0 PN 2 PG 9 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA AK1LU UT WOS:000338177400014 PM 24187014 ER PT J AU Chirumamilla, A Knodel, JJ Charlet, LD Hulke, BS Foster, SP Ode, PJ AF Chirumamilla, Anitha Knodel, Janet J. Charlet, Laurence D. Hulke, Brent S. Foster, Stephen P. Ode, Paul J. TI Ovipositional Preference and Larval Performance of the Banded Sunflower Moth (Lepidoptera: Tortricidae) and Its Larval Parasitoids on Resistant and Susceptible Lines of Sunflower (Asterales: Asteraceae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Cochylis hospes; parasitism; slow-growth-high-mortality hypothesis; trophic interactions ID NORTHERN GREAT-PLAINS; CULTIVATED SUNFLOWER; COCHYLIS-HOSPES; BACILLUS-THURINGIENSIS; SEASONAL ABUNDANCE; NATIVE SUNFLOWERS; HOST-SELECTION; GROWTH-STAGES; PYRALIDAE; PHYTOMELANIN AB Banded sunflower moth, Cochylis hospes Walsingham, is one of the most destructive seed-feeding insect pests of sunflowers, causing significant economic yield losses in the northern Great Plains. In an attempt to understand host-plant resistance mechanisms for this pest, we field-tested, over several years, the effects of seven sunflower accessions, rated as resistant to C. hospes in previous screening trials, and a susceptible control (Par 1673-2), on the ovipositional preference and larval performance of C. hospes and its larval parasitoids. Of the resistant accessions, PI 494859 was the most preferred for oviposition, receiving a significantly greater number of eggs per head than did the susceptible Par 1673-2 in 2 of 3 yr. However, the numbers of larvae, and consequently the rate of seed infestation, found in PI 494859 heads were significantly lower than those in Par 1673-2 heads over all 3 yr. Female moths laid relatively few eggs on accessions PI 170385, 291403, and 251902, compared with on Par 1673-2, resulting in lower numbers of larvae per head and lower percentages of seed damaged. No association was observed between the concentrations of two diterpenoid alcohols or two diterpenoid acids in sunflower bracts and the numbers of eggs laid on the heads of the accessions. The number of banded sunflower moth larvae and the proportion of seeds damaged were positively correlated with kaurenoic acid concentrations and negatively correlated with kauranol concentrations. A positive association between resistance to larval feeding and parasitism was found in years 2006 and 2008, with resistant accessions having significantly greater proportions of parasitized larvae than did the susceptible Par 1673-2. C1 [Chirumamilla, Anitha; Foster, Stephen P.; Ode, Paul J.] N Dakota State Univ, Dept Entomol, Fargo, ND 58108 USA. [Knodel, Janet J.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Charlet, Laurence D.; Hulke, Brent S.] ARS, No Crop Sci Lab, USDA, Fargo, ND 58105 USA. RP Ode, PJ (reprint author), Colorado State Univ, Grad Degree Program Ecol, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. EM paul.ode@colostate.edu FU National Sunflower Association FX We thank T. Gross of USDA-ARS and P. Beauzay of the Department of Plant Pathology at North Dakota State University for their assistance with field sampling and data collection. We also thank V. Chapara for his review of this manuscript. The National Sunflower Association provided a portion of the financial support for this project. NR 36 TC 1 Z9 2 U1 2 U2 16 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2014 VL 43 IS 1 BP 58 EP 68 DI 10.1603/EN13157 PG 11 WC Entomology SC Entomology GA AJ5AR UT WOS:000337693200007 PM 24367911 ER PT J AU Esquivel, JF Medrano, EG AF Esquivel, J. F. Medrano, E. G. TI Ingestion of a Marked Bacterial Pathogen of Cotton Conclusively Demonstrates Feeding by First Instar Southern Green Stink Bug (Hemiptera: Pentatomidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE feeding; first instar; Nezara viridula; Pantoea agglomerans Sc 1-R; pathogen ID NEZARA-VIRIDULA L.; STYLET SHEATH; HETEROPTERA; TRANSMISSION; PERFORMANCE; NYMPHAL; PLANT; TEMPERATURE; INDICATOR; SYMBIONT AB Long-helddogmadictates that first instars of Nezara viridula (L.) do not feed, yet recent observations of stylet activity within a food source suggest otherwise. As a cosmopolitan pest of cotton and other high-value cash crops, confirmation of feeding by first instars may ultimately influence the knowledge on biology and management strategies for this pest. To determine whether first instars feed, newly hatched nymphs were provided sterile green beans (control) or beans infected with a rifampicin- resistant marked bacterial pathogen (Pantoea agglomerans (Ewing and Fife)) of cotton. Insects were exposed to beans for 2 d, and feeding was confirmed based on detection of marked bacteria ingested by the insect. Normal bacterial flora was detected in all insects; however, control insects did not possess the marked bacteria. Of the first instars surviving on infected beans, approximate to 65% possessed the marked bacteria internally. Furthermore, the frequency of insects with marked bacteria was higher in insects collected directly from the bean surface than those that were off the bean at time of collection. Densities of innate and marked bacteria were comparable (both ranging from 10(1) to 10(3)), suggesting that the marked bacteria did not exclude preexisting bacterial flora. Marked bacteria were also detected in a subset of second instars, indicating marked bacteria were retained through the molting process after ingesting bacteria as first instars. Our findings conclusively demonstrate feeding by first instars and redefine the long-held perspective of nonfeeding by first instars. These findings may necessitate changes to crop protection strategies against feeding and vectoring of plant pathogens by N. viridula. C1 [Esquivel, J. F.] ARS, USDA, Southern Plains Agr Res Ctr, Areawide Pest Management Res Unit, College Stn, TX 77845 USA. [Medrano, E. G.] USDA ARS, SPARC, Cotton Pathol Res Unit, College Stn, TX 77845 USA. RP Esquivel, JF (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, Areawide Pest Management Res Unit, 2771 F&B Rd, College Stn, TX 77845 USA. EM jesus.esquivel@ars.usda.gov NR 31 TC 0 Z9 0 U1 1 U2 15 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2014 VL 43 IS 1 BP 110 EP 115 DI 10.1603/EN13051 PG 6 WC Entomology SC Entomology GA AJ5AR UT WOS:000337693200013 PM 24342007 ER PT J AU Poland, TM Mccullough, DG AF Poland, Therese M. Mccullough, Deborah G. TI Comparison Of Trap Types and Colors for Capturing Emerald Ash Borer Adults at Different Population Densities SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE double-decker trap; canopy trap; cis-3-hexenol; Manuka oil; Phoebe oil ID AGRILUS-PLANIPENNIS COLEOPTERA; SEX-PHEROMONE COMPONENT; BUPRESTIDAE; ATTRACTION; FAIRMAIRE; PLACEMENT; VOLATILES; TREES; (Z)-3-HEXENOL; RESPONSES AB Results of numerous trials to evaluate artificial trap designs and lures for detection of Agrilus planipennis Fairmaire, the emerald ash borer, have yielded inconsistent results, possibly because of different A. planipennis population densities in the field sites. In 2010 and 2011, we compared 1) green canopy traps, 2) purple canopy traps, 3) green double-decker traps, and 4) purple double-decker traps in sites representing a range of A. planipennis infestation levels. Traps were baited with cis-3-hexenol in both years, plus an 80: 20 mixture of Manuka and Phoebe oil (2010) or Manuka oil alone (2011). Condition of trees bearing canopy traps, A. planipennis infestation level of trees in the vicinity of traps, and number of A. planipennis captured per trap differed among sites in both years. Overall in both years, more females, males, and beetles of both sexes were captured on double-decker traps than canopy traps, and more beetles of both sexes (2010) or females (2011) were captured on purple traps than green traps. In 2010, detection rates were higher for purple (100%) and green double-decker traps (100%) than for purple (82%) or green canopy traps (64%) at sites with very low to low A. planipennis infestation levels. Captures of A. planipennis on canopy traps consistently increased with the infestation level of the canopy trap-bearing trees. Differences among trap types were most pronounced at sites with low A. planipennis densities, where more beetles were captured on purple double-decker traps than on green canopy traps in both years. C1 [Poland, Therese M.] US Forest Serv, USDA, No Res Stn, E Lansing, MI 48823 USA. [Poland, Therese M.; Mccullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Mccullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. RP Poland, TM (reprint author), US Forest Serv, USDA, No Res Stn, 1407 S Harrison Rd,Rm 220, E Lansing, MI 48823 USA. EM tpoland@fs.fed.us FU USDA-Forest Service, Special Technology Development Program; Northeastern Area Forest Health protection FX We thank Andrea Anulewicz, Jacob Bournay, James Wieferich, and Andrew Tluczek (MSU); and Tina Ciaramitaro and Tom Baweja (USDA-Forest Service), who assisted with field studies; and John Stanovick who advised on data analysis. Cooperation from the MI Department of Agriculture, Ingham County Parks, Eaton County Parks, and the MI Department of Natural Resources is gratefully acknowledged. Comments provided by Andrea Anulewicz, Brian Aukema, Yigen Chen, and two anonymous reviewers helped to improve this manuscript. Funding for this project was provided by the USDA-Forest Service, Special Technology Development Program and the Northeastern Area Forest Health protection. NR 41 TC 11 Z9 13 U1 1 U2 23 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2014 VL 43 IS 1 BP 157 EP 170 DI 10.1603/EN13137 PG 14 WC Entomology SC Entomology GA AJ5AR UT WOS:000337693200019 PM 24398125 ER PT J AU Tian, JC Long, LP Wang, XP Naranjo, SE Romeis, J Hellmich, RL Wang, P Shelton, AM AF Tian, Jun-Ce Long, Li-Ping Wang, Xiang-Ping Naranjo, Steven E. Romeis, Joerg Hellmich, Richard L. Wang, Ping Shelton, Anthony M. TI Using Resistant Prey Demonstrates That Bt Plants Producing Cry1Ac, Cry2Ab, and Cry1F Have No Negative Effects on Geocoris punctipes and Orius insidiosus SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE transgenic Bt crop; Trichoplusia ni; Spodoptera frugiperda; biological control service; prey quality ID BACILLUS-THURINGIENSIS CORN; CABBAGE-LOOPER LEPIDOPTERA; NATURAL ENEMIES; COTTON FIELDS; HETEROPTERAN PREDATORS; LABORATORY CONDITIONS; GENERALIST PREDATORS; NONTARGET ARTHROPODS; TRANSGENIC MAIZE; TOXIN AB Geocoris punctipes (Say) and Orius insidiosus (Say) are generalist predators found in a wide range of crops, including cotton (Gossypium hirsutum L.) and maize (Zea mays L.), where they provide important biological control services by feeding on an array of pests, including eggs and small larvae of caterpillars. A high percentage of cotton and maize in the United States and several other countries are transgenic cultivars that produce one or more of the insecticidal Cry proteins of Bacillus thuringiensis Berliner (Bt). Here we quantify effects of three Cry proteins on the life history of these predators over two generations when they are exposed to these Cry proteins indirectly through their prey. To eliminate the confounding prey quality effects that can be introduced by Bt-susceptible prey, we used Cry1Ac/Cry2Ab-resistant Trichoplusia ni (Hubner) and Cry1 F-resistant Spodoptera frugiperda (J.E. Smith) in a series of tri-trophic studies. Survival, development, adult mass, fecundity, and fertility were similar when predators consumed larvae feeding on Cry1Ac/Cry2Ab cotton or Cry1 F maize compared with prey feeding on isogenic or near-isogenic cotton or maize. Repeated exposure of the same initial cohort over a second generation also resulted in no differences in life-history traits when feeding on non-Bt- or Bt-fed prey. Enzyme-linked immunosorbent assay showed that predators were exposed to Bt Cry proteins from their prey and that these proteins became increasingly diluted as they moved up the food chain. Results show a clear lack of effect of three common and widespread Cry proteins on these two important predator species. The use of resistant insects to eliminate prey quality effects provides a robust and meaningful assessment of exposure and hazard. C1 [Tian, Jun-Ce; Long, Li-Ping; Wang, Xiang-Ping; Wang, Ping; Shelton, Anthony M.] Cornell Univ, New York State Agr Expt Stn, Dept Entomol, Geneva, NY 14456 USA. [Tian, Jun-Ce] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Dept Entomol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310000, Zhejiang, Peoples R China. [Long, Li-Ping] Guangxi Acad Agr Sci, Rice Res Inst, Dept Entomol, Nanning 530007, Guangxi, Peoples R China. [Wang, Xiang-Ping] Yangtze Univ, Coll Agr, Dept Entomol, Jinzhou 434001, Hubei, Peoples R China. [Naranjo, Steven E.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Romeis, Joerg] Agroscope Reckenholz Tanikon Res Stn ART, Biosafety Program, CH-8046 Zurich, Switzerland. [Hellmich, Richard L.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Hellmich, Richard L.] Iowa State Univ, Dept Entomol, Genet Lab 110, Ames, IA 50011 USA. RP Shelton, AM (reprint author), Cornell Univ, New York State Agr Expt Stn, Dept Entomol, Geneva, NY 14456 USA. EM ams5@cornell.edu FU USDA, National Institute of Food and Agriculture [2010-33522-21772] FX This project was supported by the Biotechnology Risk Assessment Program Competitive Grant 2010-33522-21772 from the USDA, National Institute of Food and Agriculture. We thank H. Collins, M. Cheung, and A. Seto for technical assistance. NR 65 TC 13 Z9 13 U1 4 U2 30 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2014 VL 43 IS 1 BP 242 EP 251 DI 10.1603/EN13184 PG 10 WC Entomology SC Entomology GA AJ5AR UT WOS:000337693200026 PM 24472212 ER PT J AU Baranowski, T O'Connor, T Johnston, C Hughes, S Moreno, J Chen, TA Meltzer, L Baranowski, J AF Baranowski, Tom O'Connor, Teresia Johnston, Craig Hughes, Sheryl Moreno, Jennette Chen, Tzu-An Meltzer, Lisa Baranowski, Janice TI School Year Versus Summer Differences in Child Weight Gain: A Narrative Review SO CHILDHOOD OBESITY LA English DT Review ID BODY-MASS INDEX; PHYSICAL-ACTIVITY; SLEEP DURATION; OBESITY PREVENTION; SEASONAL-VARIATION; SEDENTARY BEHAVIOR; DIETARY ASSESSMENT; PARENTING STYLES; RISK-FACTORS; US CHILDREN AB The causes of the current high prevalence of overweight and obesity among children are not clearly known. Schools have been implicated in the causal chain to high child obesity prevalence. Recent studies have compared school year versus summertime changes (herein called seasonal differences) in child adiposity or related phenomena. The most common seasonal pattern in six longitudinal descriptive studies was that overweight and obese children experienced accelerated gain in weight or some BMI indicator during the summer, whereas healthy weight children gained less or not at all. Four physical activity (PA) intervention studies demonstrated that school year fitness improvements were lost during the summer. One study showed that PA declined across the summer. Another study provided conflicting results of lower total energy expenditure in the summer, but no seasonal difference in total energy expenditure after adjusting for fat-free mass. This pattern of fairly rapid seasonal differences suggests that PA is the primary factor contributing to seasonal differences in weight or BMI, but the documented seasonal pattern in PA (i.e., higher in summer) does not support this relationship. Sleep duration has also been inversely related to child adiposity. Seasonal patterns in adiposity, PA, and sleep need to be clearly established separately for overweight and healthy weight children in further longitudinal research to provide a clear focus for national policy. C1 [Baranowski, Tom; O'Connor, Teresia; Johnston, Craig; Hughes, Sheryl; Moreno, Jennette; Chen, Tzu-An; Baranowski, Janice] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Meltzer, Lisa] Natl Jewish Hlth, Denver, CO USA. RP Baranowski, T (reprint author), Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU National Institute of Diabetes and Digestive and Kidney Diseases [5R01DK091254]; USDA/ARS [58-6250-6001] FX This work was funded by a grant from the National Institute of Diabetes and Digestive and Kidney Diseases (5R01DK091254). This work is also a publication of the USDA (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, TX) and had been funded, in part, with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-6001. The contents of this publication do not necessarily reflect the views or policies of the USDA nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. NR 69 TC 19 Z9 19 U1 1 U2 10 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2153-2168 EI 2153-2176 J9 CHILD OBES JI Child Obes. PD FEB PY 2014 VL 10 IS 1 BP 18 EP 24 DI 10.1089/chi.2013.0116 PG 7 WC Pediatrics SC Pediatrics GA AI5GC UT WOS:000336892400004 PM 24367922 ER PT J AU Mendoza, JA Liu, Y AF Mendoza, Jason A. Liu, Yan TI Active Commuting to Elementary School and Adiposity: An Observational Study SO CHILDHOOD OBESITY LA English DT Article ID BODY-MASS INDEX; CHILDRENS PHYSICAL-ACTIVITY; CARDIOVASCULAR-DISEASE; NEIGHBORHOOD SAFETY; PEDESTRIAN SAFETY; UNITED-STATES; OBESITY; WALKING; TRENDS; TRAVEL AB Background: Active commuting to school (ACS; walking or cycling to school) appears promising for decreasing children's obesity risk, although long-term studies are sparse. The aim was to examine whether kindergarten ACS was associated with fifth-grade adiposity. Methods: This study was a secondary analysis of the Early Childhood Longitudinal Study, Kindergarten (n = 7938). Enrollment in kindergarten (1998-1999) was nationally representative of the United States and follow-up occurred in 2004. Kindergarten ACS was the main exposure variable and fifth-grade BMI z-score was the main outcome measure. Covariates included (1) neighborhood safety and BMI z-score in kindergarten and (2) demographics (i.e., age, gender, race/ethnicity, socioeconomic status, single-vs. two-parent households, region of country, and urbanicity in fifth grade). Three interactions were included: school travel*neighborhood safety; school travel*BMI z-score (kindergarten); and school travel*socioeconomic status. Analysis of covariance accounted for the complex sampling design. Results: Kindergarten ACS was associated with lower BMI z-score in fifth grade. The interaction of school travel*neighborhood safety indicated that children from less-safe neighborhoods who did ACS in kindergarten had a lower fifth-grade BMI z-score (p <0.05) than their peers who did not do ACS in kindergarten (i.e., in terms of BMI, this difference was -0.49 kg/m(2) for children of average height in less-safe neighborhoods). Conclusion: Among children from less-safe neighborhoods, kindergarten ACS independently predicted lower BMI z-score in fifth grade among a national US cohort. Interventions and policies to increase ACS among young children, especially from unsafe neighborhoods, are warranted and should address parents' safety concerns. C1 [Mendoza, Jason A.; Liu, Yan] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Mendoza, Jason A.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Mendoza, Jason A.] Baylor Coll Med, Dan L Duncan Canc Ctr, Houston, TX 77030 USA. RP Mendoza, JA (reprint author), Univ Washington, POB 5371,Suite 400,M-S CW8-6, Seattle, WA 98145 USA. EM jason.mendoza@seattlechildrens.org OI Mendoza, Jason/0000-0003-0833-4358 FU National Cancer Institute of the National Institutes of Health (NIH) [K07CA131178, R01CA163146]; USDA [6250-51000-053] FX Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health (NIH) under award numbers K07CA131178 and R01CA163146 and the USDA under Cooperative Agreement 6250-51000-053. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH, other funders, or the authors' affiliated academic institutions. NR 60 TC 6 Z9 6 U1 3 U2 7 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2153-2168 EI 2153-2176 J9 CHILD OBES JI Child Obes. PD FEB PY 2014 VL 10 IS 1 BP 34 EP 41 DI 10.1089/chi.2013.0133 PG 8 WC Pediatrics SC Pediatrics GA AI5GC UT WOS:000336892400006 PM 24443901 ER PT J AU Gillespie, N Unthank, A Campbell, L Anderson, P Gubernick, R Weinhold, M Cenderelli, D Austin, B McKinley, D Wells, S Rowan, J Orvis, C Hudy, M Bowden, A Singler, A Fretz, E Levine, J Kirn, R AF Gillespie, Nathaniel Unthank, Amy Campbell, Lauren Anderson, Paul Gubernick, Robert Weinhold, Mark Cenderelli, Daniel Austin, Brian McKinley, Daniel Wells, Susan Rowan, Janice Orvis, Curt Hudy, Mark Bowden, Alison Singler, Amy Fretz, Eileen Levine, Jessica Kirn, Richard TI Flood Effects on Road-Stream Crossing Infrastructure: Economic and Ecological Benefits of Stream Simulation Designs SO FISHERIES LA English DT Article ID POPULATIONS; TROUT AB Stream simulation design is a geomorphic, engineering, and ecologically based approach to designing road-stream crossings that creates a natural and dynamic channel through the crossing structure similar in dimensions and characteristics to the adjacent natural channel, allowing for unimpeded passage of aquatic organisms, debris, and water during various flow conditions, including floods. A retrospective case study of the survival and failure of road-stream crossings was conducted in the upper White River watershed and the Green Mountain National Forest in Vermont following record flooding from Tropical Storm Irene in August 2011. Damage was largely avoided at two road-stream crossings where stream simulation design was implemented and extensive at multiple road-stream crossings constructed using traditional undersized hydraulic designs. Cost analyses suggest that relatively modest increases in initial investment to implement stream simulation designs yield substantial societal and economic benefits. Recommendations are presented to help agencies and stakeholders improve road-stream crossings, including increasing coordination to adopt stream simulation design methodology, increasing funding and flexibility for agencies and partners to upgrade failed crossings for flood resiliency, and expanding training workshops targeting federal, state, and local stakeholders. RESUMENel diseno de simulacion de arroyos es un enfoque geomorfico, de ingenieria y con consideraciones ecosistemicas en el que se crean pasadizos erigiendo un canal natural y dinamico entre arroyos a traves de estructuras de paso similares en dimensiones y caracteristicas al canal natural adyacente, permitiendo asi el paso irrestricto de organismos acuaticos, debris y agua durante distintas condiciones de flujo, incluyendo inundaciones. Se llevo a cabo un caso de estudio retrospectivo acerca de los exitos y fracasos de la construccion de pasadizos entre arroyos en la parte alta de la cuenca del Rio Blanco y el parque Nacional Montana Verde, en Vermont, justo despues de las inundaciones sucedidas tras el paso de la tormenta tropical Irene, en agosto de 2011. El dano fue en gran parte evitado en dos pasadizos donde se implemento el diseno de simulacion de arroyos, no asi en distintos pasadizos que fueron construidos mediante el diseno hidraulico tradicional, en los que el dano fue extensivo. El analisis de costos sugiere que incrementos relativamente pequenos en la inversion inicial, destinados a implementar un diseno de simulacion de arroyos, dan como resultado considerables beneficios sociales y economicos. Se presentan recomendaciones que podran ayudar tanto a las agencias como los participantes genuinamente interesados en el tema, a mejorar los pasadizos fluviales mediante un incremento en la coordinacion que promueva las metodologias del diseno de simulacion de arroyos, aumento de los fondos y la flexibilidad de las agencias y participantes para actualizar aquellos pasadizos cuya resiliencia a las inundaciones haya fallado y expandir los talleres de capacitacion dirigidos a participantes federales, estatales y locales. C1 [Gillespie, Nathaniel; Unthank, Amy; Campbell, Lauren; Anderson, Paul] US Forest Serv, USDA, Washington, DC 20250 USA. [Gubernick, Robert] US Forest Serv, USDA, Duluth, MN USA. [Weinhold, Mark] US Forest Serv, USDA, Glenwood Springs, CO USA. [Cenderelli, Daniel] US Forest Serv, USDA, Ft Collins, CO USA. [Austin, Brian; McKinley, Daniel] US Forest Serv, USDA, Green Mt Natl Forest, Rutland, VT USA. [Wells, Susan] US Fish & Wildlife Serv, Arlington, VA USA. [Rowan, Janice; Orvis, Curt] US Fish & Wildlife Serv, Hadley, MA USA. [Hudy, Mark] US Geol Survey, Reston, VA 22092 USA. [Bowden, Alison] Nature Conservancy, Boston, MA USA. [Singler, Amy] Amer Rivers, Northampton, MA USA. [Fretz, Eileen] Amer Rivers, Washington, DC USA. [Levine, Jessica] Nature Conservancy, Keene Valley, NY USA. [Kirn, Richard] Vermont Fish & Wildlife Dept, Roxbury, VT USA. RP Gillespie, N (reprint author), US Forest Serv, USDA, 3SE Yates Bldg,201 14th St SW, Washington, DC 20250 USA. EM ngillespie@fs.fed.us NR 48 TC 3 Z9 3 U1 3 U2 16 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 520 CHESTNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0363-2415 EI 1548-8446 J9 FISHERIES JI Fisheries PD FEB 1 PY 2014 VL 39 IS 2 BP 62 EP 76 DI 10.1080/03632415.2013.874527 PG 15 WC Fisheries SC Fisheries GA AB6WE UT WOS:000331929400010 ER PT J AU Fernandes, VC Lehotay, SJ Geis-Asteggiante, L Kwon, H Mol, HGJ van der Kamp, H Mateus, N Domingues, VF Delerue-Matos, C AF Fernandes, Virginia C. Lehotay, Steven J. Geis-Asteggiante, Lucia Kwon, Hyeyoung Mol, Hans G. J. van der Kamp, Henk Mateus, Nuno Domingues, Valentina F. Delerue-Matos, Cristina TI Analysis of pesticide residues in strawberries and soils by GC-MS/MS,LC-MS/MS and twodimensional GC-time-of-flight MS comparing organic and integrated pest management farming SO FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT LA English DT Article DE pesticide residue confirmatory analysis; chromatography; mass spectrometry; QuEChERS; strawberry; soil; farming practices ID PRESSURE GAS-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; QUECHERS SAMPLE PREPARATION; QUALITATIVE ASPECTS; MULTIRESIDUE METHOD; VEGETABLES; FRUITS; EXTRACTION; FOODS AB This study analysed 22 strawberry and soil samples after their collection over the course of 2years to compare the residue profiles from organic farming with integrated pest management practices in Portugal. For sample preparation, we used the citrate-buffered version of the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. We applied three different methods for analysis: (1) 27 pesticides were targeted using LC-MS/MS; (2) 143 were targeted using low pressure GC-tandem mass spectrometry (LP-GC-MS/MS); and (3) more than 600 pesticides were screened in a targeted and untargeted approach using comprehensive, two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC-TOF-MS). Comparison was made of the analyses using the different methods for the shared samples. The results were similar, thereby providing satisfactory confirmation of both similarly positive and negative findings. No pesticides were found in the organic-farmed samples. In samples from integrated pest management practices, nine pesticides were determined and confirmed to be present, ranging from 2 mu gkg(-1) for fluazifop-p-butyl to 50 mu gkg(-1) for fenpropathrin. Concentrations of residues in strawberries were less than European maximum residue limits. C1 [Fernandes, Virginia C.; Domingues, Valentina F.; Delerue-Matos, Cristina] Inst Politecn Porto, REQUIMTE Inst Super Engn, Oporto, Portugal. [Fernandes, Virginia C.; Mateus, Nuno] Univ Porto, CIQ, P-4100 Oporto, Portugal. [Lehotay, Steven J.; Geis-Asteggiante, Lucia] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA USA. [Geis-Asteggiante, Lucia] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Kwon, Hyeyoung] Natl Acad Agr Sci, Rural Dev Adm, Suwon, South Korea. [Mol, Hans G. J.; van der Kamp, Henk] Univ Wageningen & Res Ctr, RIKILT Inst Food Safety, Wageningen, Netherlands. RP Domingues, VF (reprint author), Inst Politecn Porto, REQUIMTE Inst Super Engn, Oporto, Portugal. EM vfd@isep.ipp.pt RI Mateus, Nuno/F-3019-2013; Delerue-Matos, Cristina/D-4990-2013; Fernandes, Virginia/G-4985-2010; OI Mateus, Nuno/0000-0002-9318-9732; Delerue-Matos, Cristina/0000-0002-3924-776X; Fernandes, Virginia/0000-0003-3979-7523; Domingues, Valentina/0000-0003-3472-849X FU Fundacao para a Ciencia e a Tecnologia [BD/47200/2008]; POPH/FSE (Programa Operacional Potencial Humano/Fundo Social Europeu); [PEst-C/EQB/LA0006/2011] FX This research was supported by a PhD grant [grant number BD/47200/2008] from the Fundacao para a Ciencia e a Tecnologia, co-financed by POPH/FSE (Programa Operacional Potencial Humano/Fundo Social Europeu); and by grant number PEst-C/EQB/LA0006/2011. NR 28 TC 8 Z9 9 U1 5 U2 54 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1944-0049 EI 1944-0057 J9 FOOD ADDIT CONTAM A JI Food Addit. Contam. Part A-Chem. PD FEB 1 PY 2014 VL 31 IS 2 BP 262 EP 270 DI 10.1080/19440049.2013.865842 PG 9 WC Chemistry, Applied; Food Science & Technology; Toxicology SC Chemistry; Food Science & Technology; Toxicology GA AB3LG UT WOS:000331691700010 PM 24224899 ER PT J AU Oduro-Yeboah, C Onwulata, C Tortoe, C Thomas-Gahring, A AF Oduro-Yeboah, Charlotte Onwulata, Charles Tortoe, Charles Thomas-Gahring, Audrey TI FUNCTIONAL PROPERTIES OF PLANTAIN, COWPEA FLOURS AND OAT FIBER IN EXTRUDED PRODUCTS SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID PHYSICAL-PROPERTIES; EXTRUSION; BANANA AB Drying effect on functional properties of two plantain and cowpea varieties and suitability of their flour blends in extruded snacks was determined. The functional and rheological behaviors of (plantain :cowpea):90:10, 80:20, 70:30, 60:40 and 50:50 blends were evaluated. The extrusion product melt temperatures were set to 90C for half-products, and 140C for fully expanded snack products. The differences in rheological properties depended on plantain and cowpea varieties. The peak viscosity for plantain flour decreased from 595.5 to 281.5 BU when blended with cowpea flour (75:25%); cowpea peak viscosities were 6 BU (Nhyira means blessings) and 13 BU (Asetenapa means good living). Paste value decreased as amount of cowpea flour blended with plantain flour increased. Pasting properties of the extrusion blends were significantly different (P < 0.05) depending on the blend ratios. The level of cowpea added affected the paste, hardness properties and the expansion height of the extruded products. C1 [Oduro-Yeboah, Charlotte; Tortoe, Charles] CSIR, Food Res Inst, Accra 233, Ghana. [Onwulata, Charles; Thomas-Gahring, Audrey] USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA. RP Oduro-Yeboah, C (reprint author), CSIR, Food Res Inst, POB M20, Accra 233, Ghana. EM charlotteoduroyeboah@yahoo.com FU United States Department of Agriculture-Agricultural Research Service-Eastern Regional Research Center [-58-1935-9-174F]; CSIR-Food Research Institute [-58-1935-9-174F] FX We are grateful to Raymond-Hadley Corp., Spencer, NY 14883, Bob's Red Mill Natural Foods, Inc., Milwaukie, OR, and Sun Opta Ingredient Group (Chelmsford, MA) for the supply of the plantain flour, oat fiber and white bean flour. The contribution of United States Department of Agriculture-Agricultural Research Service-Eastern Regional Research Center and CSIR-Food Research Institute is appreciated. Grant Agreement -58-1935-9-174F. NR 29 TC 3 Z9 3 U1 1 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD FEB PY 2014 VL 38 IS 1 BP 347 EP 355 DI 10.1111/j.1745-4549.2012.00782.x PG 9 WC Food Science & Technology SC Food Science & Technology GA AH6RR UT WOS:000336258900038 ER PT J AU Khir, R Pan, ZL Thompson, JF El-Sayed, AS Hartsough, BR El-Amir, MS AF Khir, Ragab Pan, Zhongli Thompson, James F. El-Sayed, Adel S. Hartsough, Bruce R. El-Amir, Mohamed S. TI MOISTURE REMOVAL CHARACTERISTICS OF THIN LAYER ROUGH RICE UNDER SEQUENCED INFRARED RADIATION HEATING AND COOLING SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID QUALITY AB The objective of this study was to investigate the moisture removal characteristics of thin layer rough rice heated by infrared (IR) and cooled with various cooling methods. Thin layer rough rice samples with different initial moisture contents (MCs) were heated using a catalytic IR emitter for four exposure times and radiation intensities. High heating rate and moisture removal were achieved during the IR heating period. After heating, more moisture removal was achieved during the cooling period. The achieved grain temperatures ranged from 35.1 to 68.4C under the tested heating conditions. The vacuum and forced air cooling methods removed more moisture than did the natural cooling. When rice with 25.7% MC was heated by IR, MC was reduced by 3.2, 3.5, and 3.8 percentage points for rice heated to 63.5C at the IR intensity of 5348 W/m(2) for 120 s followed by natural cooling for 40 min, forced air cooling for 5 min and vacuum cooling for 10 min, respectively. C1 [Khir, Ragab; Pan, Zhongli; Thompson, James F.; Hartsough, Bruce R.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Pan, Zhongli] USDA ARS WRRC, Proc Foods Res Unit, Albany, CA 94710 USA. [Khir, Ragab; El-Sayed, Adel S.; El-Amir, Mohamed S.] Suez Canal Univ, Fac Agr, Dept Agr Engn, Ismailia, Egypt. RP Pan, ZL (reprint author), USDA ARS WRRC, Proc Foods Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM Zhongli.Pan@ars.usda.gov NR 32 TC 8 Z9 8 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD FEB PY 2014 VL 38 IS 1 BP 430 EP 440 DI 10.1111/j.1745-4549.2012.00791.x PG 11 WC Food Science & Technology SC Food Science & Technology GA AH6RR UT WOS:000336258900047 ER PT J AU Oliveira, ACM Bechtel, PJ Morey, A Brenner, K Lapis, TJ AF Oliveira, Alexandra C. M. Bechtel, Peter J. Morey, Amit Brenner, Kathryn Lapis, Trina J. TI CHEMICAL COMPOSITION OF SPINY DOGFISH (SQUALUS SUCKLEYI) HARVESTED IN ALASKA SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID ACANTHIAS; LIVER; SEPARATION; QUALITY; PROTEIN; STORAGE; SHARK; ACID AB Spiny dogfish is an underutilized yet abundant species harvested as bycatch in Alaska. The research goal was to determine the nutritional composition of dogfish by-products and compare results with headed and gutted (H&G) dogfish. Large differences existed in lipid content between the heads (1.8% w/w), H&G (9.2% w/w) and livers (65.4% w/w). The overall omega-3 fatty acid content ranged from 75.7 to 162 mg/g lipids in all tissues. Cholesterol content was significantly higher in livers (602 mg/100 g tissue) than in heads or H&G. Values for three potentially limiting amino acids ranged in the tissues from 2.6 to 2.8% w/w for methionine, 4.2 to 5.2% w/w for threonine and 6.9 to 8.8% w/w for lysine. Dogfish livers are a good source of fish oil, while heads and H&G are rich in protein and low in ash, which make these two products ideal raw materials for production of fish hydrolysates. C1 [Oliveira, Alexandra C. M.; Morey, Amit; Brenner, Kathryn; Lapis, Trina J.] Univ Alaska, Sch Fisheries & Ocean Sci, Kodiak Seafood & Marine Sci Ctr, Kodiak, AK 99615 USA. [Bechtel, Peter J.] Univ Alaska, USDA ARS Subarctic Agr Res Unit, Fairbanks, AK 99701 USA. RP Oliveira, ACM (reprint author), Univ Alaska, Sch Fisheries & Ocean Sci, Kodiak Seafood & Marine Sci Ctr, 118 Trident Way, Kodiak, AK 99615 USA. EM acoliveira@alaska.edu NR 33 TC 0 Z9 0 U1 2 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD FEB PY 2014 VL 38 IS 1 BP 600 EP 606 DI 10.1111/jfpp.12008 PG 7 WC Food Science & Technology SC Food Science & Technology GA AH6RR UT WOS:000336258900066 ER PT J AU Yuk, HG Sampedro, F Fan, XT Geveke, DJ AF Yuk, Hyun Gyun Sampedro, Fernando Fan, Xuetong Geveke, David J. TI NONTHERMAL PROCESSING OF ORANGE JUICE USING A PILOT-PLANT SCALE SUPERCRITICAL CARBON DIOXIDE SYSTEM WITH A GAS-LIQUID METAL CONTACTOR SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID FREQUENCY ELECTRIC-FIELDS; LACTOBACILLUS-PLANTARUM; INACTIVATION KINETICS; ESCHERICHIA-COLI; APPLE CIDER; PRESSURE; MICROORGANISMS; QUALITY; CLOUD; FOODS AB This study compared the quality of fresh orange juice to that of supercritical carbon dioxide (SCCO2)-processed juice and equivalently thermally processed juice in terms of microbial lethality. A pilot-plant scale SCCO2 unit with a gas-liquid metal contactor processed juice with a CO2 concentration of ca. 8.5 wt % at 42C for 20 min. Thermal processing was conducted at 70C for 7.2 s. The number of naturally occurring microorganisms decreased from ca. 2.0-3.0 x 10(3) to 18-28 cfu/mL after both SCCO2 and thermal processing. No noticeable changes in pH, degrees Brix, titratable acidity and ascorbic acid content were observed between processed and unprocessed juice. SCCO2 and thermal processing inactivated 46.5 and 86.4% of pectin methylesterase, respectively. The cloud stability of the SCCO2-processed juice was greatly enhanced compared with fresh and thermally processed juices. This study demonstrated that SCCO2 processing can improve the microbial quality of orange juice without deterioration, suggesting the potential for commercialization. C1 [Yuk, Hyun Gyun] Natl Univ Singapore, Dept Chem, Food Sci & Technol Programme, Singapore 117548, Singapore. [Sampedro, Fernando; Fan, Xuetong] ARS, Residue Chem & Predict Microbiol Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Geveke, David J.] ARS, Food Safety & Intervent Technol Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Geveke, DJ (reprint author), ARS, Food Safety & Intervent Technol Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM david.geveke@ars.usda.gov NR 40 TC 3 Z9 3 U1 5 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD FEB PY 2014 VL 38 IS 1 BP 630 EP 638 DI 10.1111/jfpp.12013 PG 9 WC Food Science & Technology SC Food Science & Technology GA AH6RR UT WOS:000336258900070 ER PT J AU Appell, M Jackson, MA Wang, LJC Ho, CH Mueller, A AF Appell, Michael Jackson, Michael A. Wang, Lijuan C. Ho, Che-Hin Mueller, Anja TI Determination of fusaric acid in maize using molecularly imprinted SPE clean-up SO JOURNAL OF SEPARATION SCIENCE LA English DT Article DE Food safety; HPLC; Ion pair chromatography; Molecularly imprinted polymers; Mycotoxins ID SOLID-PHASE EXTRACTION; POLYMER; BANANA; SWINE AB A new LC method to detect fusaric acid (FA) in maize is reported based on a molecularly imprinted SPE clean-up using mimic-templated molecularly imprinted polymers. Picolinic acid was used as a toxin analog for imprinting polymers during a thermolytic synthesis. Both acidic and basic functional monomers were predicted to have favorable binding interactions by MP2 ab initio calculations. Imprinted polymers synthesized with methacrylic acid or 2-dimethylaminoethylmethacrylate exhibited imprinting effects in SPE analysis. FA levels were determined using RP ion-pairing chromatography with diode-arrayUV detection and tetrabutylammonium hydrogen sulfate in the mobile phase. A method was developed to detect FA in maize using molecularly imprinted SPE analysis within the range of 1-100 mu g/g with recoveries between 83.9 and 92.1%. C1 [Appell, Michael; Wang, Lijuan C.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Jackson, Michael A.] ARS, Renewable Prod Technol Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Ho, Che-Hin; Mueller, Anja] Cent Michigan Univ, Dept Chem, Mt Pleasant, MI 48859 USA. RP Appell, M (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM michael.appell@ars.usda.gov NR 17 TC 11 Z9 12 U1 2 U2 22 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY SN 1615-9306 EI 1615-9314 J9 J SEP SCI JI J. Sep. Sci. PD FEB PY 2014 VL 37 IS 3 BP 281 EP 286 DI 10.1002/jssc.201301065 PG 6 WC Chemistry, Analytical SC Chemistry GA AI0IC UT WOS:000336529100013 PM 24227222 ER PT J AU Zhao, F Huang, YB Guo, YQ Reddy, KN Lee, MA Fletcher, RS Thomson, SJ AF Zhao, Feng Huang, Yanbo Guo, Yiqing Reddy, Krishna N. Lee, Matthew A. Fletcher, Reginald S. Thomson, Steven J. TI Early Detection of Crop Injury from Glyphosate on Soybean and Cotton Using Plant Leaf Hyperspectral Data SO REMOTE SENSING LA English DT Article DE crop injury; herbicide; glyphosate; leaf reflectance; spectral indices; sensitivity analysis; canonical analysis ID RADIATIVE-TRANSFER MODELS; OPTICAL-PROPERTIES MODEL; SENSITIVITY-ANALYSIS; REFLECTANCE MEASUREMENTS; WATER-CONTENT; PROSPECT; DRIFT; VEGETATION; INDEXES; INDICATORS AB In this paper, we aim to detect crop injury from glyphosate, a herbicide, by both traditionally used spectral indices and newly extracted features with leaf hyperspectral reflectance data for non-Glyphosate-Resistant (non-GR) soybean and non-GR cotton. The new features were extracted by canonical analysis technique, which could provide the largest separability to distinguish the injured leaves from the healthy ones. Spectral bands used for constructing these new features were selected based on the sensitivity analysis results of a physically-based leaf radiation transfer model (leaf optical PROperty SPECTra model, PROSPECT), which could help extend the effectiveness of these features to a wide range of leaf structures and growing conditions. This approach has been validated with greenhouse measured data acquired in glyphosate treatment experiments. Results indicated that glyphosate injury could be detected by NDVI (Normalized Difference Vegetation Index), RVI (Ratio Vegetation Index), SAVI (Soil Adjusted Vegetation Index), and DVI (Difference Vegetation Index) in 48 h After the Treatment (HAT) for soybean and in 72 HAT for cotton, but the other spectral indices either showed little use for separation, or did not show consistent separation for healthy and injured soybean and cotton. Compared with the traditional spectral indices, the new features were more feasible for the early detection of glyphosate injury, with leaves sprayed with a higher rate of glyphosate solution having larger feature values. This trend became more and more pronounced with time. Leaves sprayed with different glyphosate rates showed some separability 24 HAT using the new features and could be totally distinguished at and beyond 48 HAT for both soybean and cotton. These findings demonstrated the feasibility of applying leaf hyperspectral reflectance measurements for the early detection of glyphosate injury using these newly proposed features. C1 [Zhao, Feng; Guo, Yiqing] Beihang Univ, Sch Instrumentat Sci & Optoelect Engn, Beijing 100191, Peoples R China. [Huang, Yanbo; Reddy, Krishna N.; Lee, Matthew A.; Fletcher, Reginald S.; Thomson, Steven J.] ARS, USDA, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Zhao, F (reprint author), Beihang Univ, Sch Instrumentat Sci & Optoelect Engn, Beijing 100191, Peoples R China. EM zhaofeng@buaa.edu.cn; Yanbo.Huang@ars.usda.gov; yguo_buaa@163.com; Krishna.Reddy@ars.usda.gov; leefromms@gmail.com; Reginald.Fletcher@ars.usda.gov; Steve.Thomson@ars.usda.gov FU Chinese Natural Science Foundation [41371325, 40901156]; State Key Laboratory of Remote Sensing Science [2009kfjj022]; Civil Aerospace Technology Pre-research Project of China [D040201-03] FX This work is jointly supported by the Chinese Natural Science Foundation under Project 41371325 and 40901156, the State Key Laboratory of Remote Sensing Science (Grant No. 2009kfjj022), and the Civil Aerospace Technology Pre-research Project of China (Grant No. D040201-03). This work also contributes to Integrated Sustainable Crop Production Systems for the United States Department of Agriculture, Agricultural Research Service, National Program 305, Crop Production. Thanks go to Efren Ford and Paige Goodlett for their technical assistance. The authors are thankful to the anonymous reviewers who provided constructive comments to improve this manuscript. NR 44 TC 8 Z9 8 U1 4 U2 22 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD FEB PY 2014 VL 6 IS 2 BP 1538 EP 1563 DI 10.3390/rs6021538 PG 26 WC Remote Sensing SC Remote Sensing GA AH4JB UT WOS:000336092100030 ER PT J AU Vincent, A Awada, L Brown, I Chen, H Claes, F Dauphin, G Donis, R Culhane, M Hamilton, K Lewis, N Mumford, E Nguyen, T Parchariyanon, S Pasick, J Pavade, G Pereda, A Peiris, M Saito, T Swenson, S Van Reeth, K Webby, R Wong, F Ciacci-Zanella, J AF Vincent, A. Awada, L. Brown, I. Chen, H. Claes, F. Dauphin, G. Donis, R. Culhane, M. Hamilton, K. Lewis, N. Mumford, E. Nguyen, T. Parchariyanon, S. Pasick, J. Pavade, G. Pereda, A. Peiris, M. Saito, T. Swenson, S. Van Reeth, K. Webby, R. Wong, F. Ciacci-Zanella, J. TI Review of Influenza A Virus in Swine Worldwide: A Call for Increased Surveillance and Research SO ZOONOSES AND PUBLIC HEALTH LA English DT Review DE Influenza A virus; swine; one health; surveillance ID PANDEMIC H1N1 2009; UNITED-STATES; GENETIC-CHARACTERIZATION; SOUTHERN CHINA; H3N2 VIRUSES; ANTIGENIC CHARACTERIZATION; AVIAN-LIKE; PIGS; REASSORTANT; HUMANS AB Pigs and humans have shared influenza A viruses (IAV) since at least 1918, and many interspecies transmission events have been documented since that time. However, despite this interplay, relatively little is known regarding IAV circulating in swine around the world compared with the avian and human knowledge base. This gap in knowledge impedes our understanding of how viruses adapted to swine or man impacts the ecology and evolution of IAV as a whole and the true impact of swine IAV on human health. The pandemic H1N1 that emerged in 2009 underscored the need for greater surveillance and sharing of data on IAV in swine. In this paper, we review the current state of IAV in swine around the world, highlight the collaboration between international organizations and a network of laboratories engaged in human and animal IAV surveillance and research, and emphasize the need to increase information in high-priority regions. The need for global integration and rapid sharing of data and resources to fight IAV in swine and other animal species is apparent, but this effort requires grassroots support from governments, practicing veterinarians and the swine industry and, ultimately, requires significant increases in funding and infrastructure. C1 [Vincent, A.] USDA ARS NADC, Virus & Prion Res Unit, Ames, IA 50010 USA. [Awada, L.; Hamilton, K.; Pavade, G.] World Org Anim Hlth OIE, Paris, France. [Brown, I.] Anim Hlth & Vet Labs Agcy, Weybridge, Surrey, England. [Chen, H.] Harbin Vet Res Inst, Harbin, Peoples R China. [Claes, F.; Dauphin, G.] Food & Agr Org United Nations FAO, Rome, Italy. [Donis, R.] CDC, Atlanta, GA 30333 USA. [Culhane, M.] Univ Minnesota, Vet Diagnost Lab, St Paul, MN 55108 USA. [Lewis, N.] Univ Cambridge, Dept Zool, Cambridge, England. [Mumford, E.] WHO, CH-1211 Geneva, Switzerland. [Nguyen, T.] Natl Ctr Vet Diagnost, Dept Anim Hlth, Hanoi, Vietnam. [Parchariyanon, S.] Natl Inst Anim Hlth, Bangkok, Thailand. [Pasick, J.] Canadian Food Inspect Agcy, Winnipeg, MB, Canada. [Pereda, A.] Inst Virol INTA, Buenos Aires, DF, Argentina. [Peiris, M.] Univ Hong Kong, Hong Kong, Hong Kong, Peoples R China. [Saito, T.] Natl Inst Anim Hlth, Ibaraki, Japan. [Swenson, S.] USDA APHIS NVSL, Ames, IA USA. [Van Reeth, K.] Univ Ghent, B-9000 Ghent, Belgium. [Webby, R.] St Jude Childrens Res Hosp, Memphis, TN 38105 USA. [Wong, F.] CSIRO Livestock Ind, Australian Anim Hlth Lab, Geelong, Vic, Australia. [Ciacci-Zanella, J.] EMBRAPA Swine & Poultry, Concordia, SC, Brazil. RP Vincent, A (reprint author), USDA ARS NADC, Virus & Prion Res Unit, 1920 Dayton Ave, Ames, IA 50010 USA. EM amy.vincent@ars.usda.gov RI Wong, Frank/H-1950-2013; Brown, Ian/E-1119-2011; APHA, Staff publications/E-6082-2010; Zanella, Janice/C-3632-2014; OI Mumford, Elizabeth/0000-0002-9548-9675 NR 87 TC 66 Z9 69 U1 3 U2 47 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD FEB PY 2014 VL 61 IS 1 BP 4 EP 17 DI 10.1111/zph.12049 PG 14 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA AI1FI UT WOS:000336595300002 PM 23556412 ER PT J AU Kim, BI Blanton, JD Gilbert, A Castrodale, L Hueffer, K Slate, D Rupprecht, CE AF Kim, B. I. Blanton, J. D. Gilbert, A. Castrodale, L. Hueffer, K. Slate, D. Rupprecht, C. E. TI A Conceptual Model for the Impact of Climate Change on Fox Rabies in Alaska, 1980-2010 SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Climate change; rabies; red fox; arctic fox; epizootiology ID ARCTIC FOX; ALOPEX-LAGOPUS; POPULATION-DYNAMICS; VULPES-VULPES; RED FOXES; SEA-ICE; VIRUS; COMPETITION; ONTARIO; ELIMINATION AB The direct and interactive effects of climate change on host species and infectious disease dynamics are likely to initially manifest at latitudinal extremes. As such, Alaska represents a region in the United States for introspection on climate change and disease. Rabies is enzootic among arctic foxes (Vulpes lagopus) throughout the northern polar region. In Alaska, arctic and red foxes (Vulpes vulpes) are reservoirs for rabies, with most domestic animal and wildlife cases reported from northern and western coastal Alaska. Based on passive surveillance, a pronounced seasonal trend in rabid foxes occurs in Alaska, with a peak in winter and spring. This study describes climatic factors that may be associated with reported cyclic rabies occurrence. Based upon probabilistic modelling, a stronger seasonal effect in reported fox rabies cases appears at higher latitudes in Alaska, and rabies in arctic foxes appear disproportionately affected by climatic factors in comparison with red foxes. As temperatures continue a warming trend, a decrease in reported rabid arctic foxes may be expected. The overall epidemiology of rabies in Alaska is likely to shift to increased viral transmission among red foxes as the primary reservoir in the region. Information on fox and lemming demographics, in addition to enhanced rabies surveillance among foxes at finer geographic scales, will be critical to develop more comprehensive models for rabies virus transmission in the region. C1 [Kim, B. I.] Emory Univ, Rollins Sch Publ Hlth, Atlanta, GA 30322 USA. [Kim, B. I.; Blanton, J. D.; Gilbert, A.; Rupprecht, C. E.] Ctr Dis Control & Prevent, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Castrodale, L.] Alaska Div Publ Hlth, Anchorage, AK USA. [Hueffer, K.] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK USA. [Hueffer, K.] Univ Alaska Fairbanks, Dept Biol & Wildlife, Fairbanks, AK USA. [Slate, D.] Wildlife Serv, Natl Rabies Management Program, USDA, Manchester, NH USA. RP Blanton, JD (reprint author), Ctr Dis Control & Prevent, Div High Consequence Pathogens & Pathol, 1600 Clifton Rd NE,Mailstop G33, Atlanta, GA 30333 USA. EM asi5@cdc.gov FU National Center for Research Resources [5P20RR016466-12]; National Institute of General Medical Sciences, National Institutes of Health [8 P20 GM103395-12] FX The authors thank the Alaska Division of Public Health for their contribution in collecting and maintaining rabies surveillance data; Dustyn Palmer and Jessie Dyer, CDC Rabies program, for assistance with providing map figures; Ivan Kuzmin, CDC Rabies program for reviewing the manuscript; and Christine Fehlner-Gardiner, Canadian Food Inspection Agency Centre of Expertise for Rabies, for review of the manuscript. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of their institutions. Karsten Hueffer is supported by grants from the National Center for Research Resources (5P20RR016466-12) and the National Institute of General Medical Sciences (8 P20 GM103395-12) from the National Institutes of Health. NR 52 TC 5 Z9 5 U1 6 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD FEB PY 2014 VL 61 IS 1 BP 72 EP 80 DI 10.1111/zph.12044 PG 9 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA AI1FI UT WOS:000336595300009 PM 23452510 ER PT J AU Ortega-Azorin, C Sorli, JV Estruch, R Asensio, EM Coltell, O Gonzalez, JI Martinez-Gonzalez, MA Ros, E Salas-Salvado, J Fito, M Aros, F Lapetra, J Serra-Majem, L Ruiz-Gutierrez, V Gomez-Gracia, E Fiol, M Flores, G Pinto, X Saiz, C Ordovas, JM Corella, D AF Ortega-Azorin, Carolina Sorli, Jose V. Estruch, Ramon Asensio, Eva M. Coltell, Oscar Gonzalez, Jose I. Martinez-Gonzalez, Miguel Angel Ros, Emilio Salas-Salvado, Jordi Fito, Montserrat Aros, Fernando Lapetra, Jose Serra-Majem, Lluis Ruiz-Gutierrez, Valentina Gomez-Gracia, Enrique Fiol, Miquel Flores, Gemma Pinto, Xavier Saiz, Carmen Ordovas, Jose M. Corella, Dolores TI Amino Acid Change in the Carbohydrate Response Element Binding Protein Is Associated With Lower Triglycerides and Myocardial Infarction Incidence Depending on Level of Adherence to the Mediterranean Diet in the PREDIMED Trial SO CIRCULATION-CARDIOVASCULAR GENETICS LA English DT Article ID CORONARY-ARTERY-DISEASE; PLASMA TRIGLYCERIDES; LIPID CONCENTRATIONS; INSULIN-RESISTANCE; HEPATIC STEATOSIS; BLOOD-LIPIDS; MLXIPL GENE; OB/OB MICE; CHREBP; LOCI C1 [Ortega-Azorin, Carolina; Sorli, Jose V.; Asensio, Eva M.; Gonzalez, Jose I.; Saiz, Carmen; Corella, Dolores] Univ Valencia, Sch Med, Dept Prevent Med, Valencia 46010, Spain. [Ortega-Azorin, Carolina; Sorli, Jose V.; Estruch, Ramon; Asensio, Eva M.; Coltell, Oscar; Gonzalez, Jose I.; Martinez-Gonzalez, Miguel Angel; Ros, Emilio; Salas-Salvado, Jordi; Fito, Montserrat; Aros, Fernando; Lapetra, Jose; Serra-Majem, Lluis; Ruiz-Gutierrez, Valentina; Gomez-Gracia, Enrique; Fiol, Miquel; Pinto, Xavier; Corella, Dolores] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr, Madrid, Spain. [Estruch, Ramon] Hosp Clin Barcelona, IDIBAPS, Dept Internal Med, Barcelona, Spain. [Ros, Emilio] Hosp Clin Barcelona, IDIBAPS, Endocrinol & Nutr Serv, Lipid Clin, Barcelona, Spain. [Coltell, Oscar] Jaume I Univ, Dept Comp Languages & Syst, Castellon de La Plana, Spain. [Martinez-Gonzalez, Miguel Angel] Univ Navarra, Sch Med, Dept Prevent Med & Publ Hlth, E-31080 Pamplona, Spain. [Salas-Salvado, Jordi] Univ Rovira & Virgili, Fac Med, Human Nutr Unit, E-43201 Reus, Spain. [Fito, Montserrat] Hosp del Mar Invest Med, Cardiovasc Risk & Nutr Rsrch Grp, Barcelona, Spain. [Aros, Fernando] Hosp Txagorritxu, Dept Cardiol, Vitoria, Spain. [Lapetra, Jose] San Pablo Hlth Ctr, Dept Family Med, Seville, Spain. [Serra-Majem, Lluis] Las Palmas de Gran Canaria Univ, Dept Clin Sci, Las Palmas Gran Canaria, Spain. [Ruiz-Gutierrez, Valentina] CSIC, Inst Grasa, E-41080 Seville, Spain. [Gomez-Gracia, Enrique] Univ Malaga, Sch Med, Dept Epidemiol, Malaga, Spain. [Fiol, Miquel] Hosp Son Dureta, Univ Inst Hlth Sci, Palma De Mallorca, Spain. [Flores, Gemma] Catalan Inst Hlth, Primary Care Div, Barcelona, Spain. [Pinto, Xavier] Hosp Univ Bellvitge, Lipids & Vasc Risk Unit, Barcelona, Spain. [Ordovas, Jose M.] CNIC, Dept Cardiovasc Epidemiol & Populat Genet, Madrid, Spain. [Ordovas, Jose M.] IMDEA Alimentac, Madrid, Spain. [Ordovas, Jose M.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. RP Corella, D (reprint author), Univ Valencia, Genet & Mol Epidemiol Unit, Blasco Ibanez 15, Valencia 46010, Spain. EM dolores.corella@uv.es RI Lapetra, Jose/F-2552-2015; Fiol, Miguel/F-6793-2016; IBIS, ATENCION PRIMARIA/I-3524-2016; Fito, Montserrat/C-1822-2012; Salas-Salvado, Jordi/C-7229-2017 OI Fito, Montserrat/0000-0002-1817-483X; Salas-Salvado, Jordi/0000-0003-2700-7459 FU Ministry of Economy; Fondo Europeo de Desarrollo Regional [CIBER 06/03, PI07-0954, CNIC-06, PI11/02505, SAF2009-12304, AGL2010-22319-C03-03]; US Department of Agriculture Research Service, USA [53-K06-5, 53-K06-6, 53-K06-7, 53-K06-8, 53-K06-9, 53-K06-10, 58-1950-9-001]; Generalitat Valenciana, Spain [AP-042/11, ACOMP/2012/190, ACOMP/2013/159, ACOMP/213/165]; Instituto de Salud Carlos III; Catalan Government [CP06/00100] FX Study supported by the Ministry of Economy and the Fondo Europeo de Desarrollo Regional (projects CIBER 06/03, PI07-0954, CNIC-06, PI11/02505, SAF2009-12304 and AGL2010-22319-C03-03), by contracts 53-K06-5 to 10 and 58-1950-9-001 from the US Department of Agriculture Research Service, USA and by the Generalitat Valenciana, Spain (AP-042/11, ACOMP/2012/190, ACOMP/2013/159, and ACOMP/213/165). M. Fito was supported by the Instituto de Salud Carlos III and the Catalan Government, CP06/00100. NR 37 TC 10 Z9 10 U1 1 U2 9 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1942-3268 J9 CIRC-CARDIOVASC GENE JI Circ.-Cardiovasc. Genet. PD FEB PY 2014 VL 7 IS 1 BP 49 EP 58 PG 10 WC Cardiac & Cardiovascular Systems; Genetics & Heredity SC Cardiovascular System & Cardiology; Genetics & Heredity GA AA9IC UT WOS:000331406000009 PM 24448738 ER PT J AU Quesada, T Resende, MFR Munoz, P Wegrzyn, JL Neale, DB Kirst, M Peter, GF Gezan, SA Nelson, CD Davis, JM AF Quesada, Tania Resende, Marcio F. R., Jr. Munoz, Patricio Wegrzyn, Jill L. Neale, David B. Kirst, Matias Peter, Gary F. Gezan, Salvador A. Nelson, C. Dana Davis, John M. TI Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine SO FORESTS LA English DT Article DE Pinus taeda; major gene resistance; gene-for-gene interaction; single nucleotide polymorphism (SNP); BayesC pi; Bayesian Association with Missing Data (BAMD); logarithm of the odds (LOD) ID MULTILOCUS GENOTYPE DATA; DISEASE RESISTANCE; TAEDA L.; POPULATION-STRUCTURE; GENOMIC SELECTION; R-GENE; IDENTIFICATION; MARKERS; IMPROVEMENT; VIRULENCE AB Fusiform rust resistance can involve gene-for-gene interactions where resistance (Fr) genes in the host interact with corresponding avirulence genes in the pathogen, Cronartium quercuum f.sp. fusiforme (Cqf). Here, we identify trees with Fr genes in a loblolly pine population derived from a complex mating design challenged with two Cqf inocula (one gall and 10 gall mixtures). We used single nucleotide polymorphism (SNP) genotypes at sufficient density to ensure linkage between segregating markers and Fr genes identifying SNPs that explained high proportions of variance in disease incidence using BayesC pi, that also were significant using Bayesian Association with Missing Data (BAMD) software. Two SNPs mapped near Fr1 and generated significant LOD scores in single marker regression analyses for Fr1/fr1 parent 17 as well as four other parents. One SNP mapped near Fr8 and was significant for parent 28. Two SNPs mapped to linkage groups not previously shown to contain Fr genes and were significant for three parents. Parent 2 showed evidence of Fr gene stacking. Our results suggest that it is feasible to identify trees segregating for Fr genes, and to map Fr genes, based on parental analysis of SNPs that cosegregate with disease incidence in designed resistance screening trials. C1 [Quesada, Tania; Kirst, Matias; Peter, Gary F.; Gezan, Salvador A.; Davis, John M.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Resende, Marcio F. R., Jr.] Univ Florida, Genet & Genom Grad Program, Gainesville, FL 32611 USA. [Munoz, Patricio] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Wegrzyn, Jill L.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA. [Neale, David B.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Kirst, Matias; Peter, Gary F.; Davis, John M.] Univ Florida, Genet Inst, Gainesville, FL 32611 USA. [Nelson, C. Dana] US Forest Serv, USDA, Southern Res Stn, Southern Inst Forest Genet, Saucier, MS 39574 USA. RP Davis, JM (reprint author), Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. EM tquesada@ufl.edu; mresende@ufl.edu; p.munoz@ufl.edu; jill.wegrzyn@uconn.edu; dbneale@ucdavis.edu; mkirst@ufl.edu; gfpeter@ufl.edu; sgezan@ufl.edu; dananelson@fs.fed.us; jmdavis@ufl.edu FU USDA-CSREES-IFAFS [2001-52100-11315]; National Science Foundation [IOS-PGRP-0501763] FX This work was supported by USDA-CSREES-IFAFS (grant 2001-52100-11315) and by the National Science Foundation (IOS-PGRP-0501763). The Forest Biology Research Cooperative at the University of Florida provided the plant materials, and Dr. Robert Schmidt (retired, University of Florida) provided inoculum. We thank Carol Young (retired, US Forest Service, Resistance Screening Center), Kathy Smith (Southern Institute of Forest Genetics) and Chris Dervinis (University of Florida) for technical assistance. Finally, we thank Henry Amerson and Thomas Kubisiak for making their Fr gene mapping information available for cross-reference during the preparation of this manuscript. NR 42 TC 6 Z9 6 U1 2 U2 20 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD FEB PY 2014 VL 5 IS 2 BP 347 EP 362 DI 10.3390/f5020347 PG 16 WC Forestry SC Forestry GA AH4GS UT WOS:000336086000008 ER PT J AU Williams, TM Amatya, DM Hitchcock, DR Edwards, AE AF Williams, Thomas M. Amatya, Devendra M. Hitchcock, Daniel R. Edwards, Amy E. TI Streamflow and Nutrients from a Karst Watershed with a Downstream Embayment: Chapel Branch Creek SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article DE Lake Marion; Streamflow; Nitrogen; Subsurface flow; Runoff coefficient (ROC); Groundwater; Sinkhole; Water balance; Total maximum daily load (TMDL); Phosphorus ID QUALITY; CAROLINA; SWAT AB Understanding sources of streamflow and nutrient concentrations are fundamental for the assessment of pollutant loadings that can lead to water quality impairments. The objective of this study was to evaluate the discharge of three main tributaries, draining different land uses with karst features, as well as their combined influences on total nitrogen (TN) and total phosphorus (TP) levels in reservoir-like embayment (R-E) on a stream entering Lake Marion, South Carolina. From 2007-2009, hydrology, TN, and TP data were collected from the 1,555-ha Chapel Branch Creek (CBC) watershed. In general, monthly streamflow in all tributaries was found to be -10% of rainfall, and as little as 0.1% in the smallest tributary. The third tributary flowed into a cave system and discharged via a cave spring (CS) into the embayment while gaining a sustained groundwater flow from a second cave (GW) system. The CS flow was substantially larger than the flow measured in the other tributaries. The small amount of rainfall that became surface flow and the large flow at the cave spring indicated a significant water loss from the surface watershed to subsurface flow or a groundwater source area substantially larger than the surface watershed. Nutrient concentrations in flows from tributaries draining various land uses were not significantly different (alpha=0.05) for most of the locations. A simple water balance was developed to estimate the R-E outflow to Lake Marion using measured discharges from three tributaries, change in storage computed using a bathymetric survey, daily lake level changes, rainfall, and computed evaporation. Mean monthly TN and TP concentrations in the embayment were substantially lower than the observed means from the two tributary outlets and the CS into the embayment, indicating a loss in the embayment. The second cave system at CS, representing an unknown subsurface drainage area, was the source of nearly 50% of TP loading, over 50% of flow, and over 70% of TN loading to CBC. These results may have implications in water quality management of the CBC watershed. C1 [Williams, Thomas M.; Hitchcock, Daniel R.] Clemson Univ, Baruch Inst Coastal Ecol & Forest Sci, Georgetown, SC 29442 USA. [Amatya, Devendra M.] USDA Forest Serv, Ctr Forested Wetlands Res, Cordesville, SC 29434 USA. [Edwards, Amy E.] Florida A&M Univ, Sch Environm Sci, Tallahassee, FL 32307 USA. RP Amatya, DM (reprint author), USDA Forest Serv, Ctr Forested Wetlands Res, 3734 Highway 402, Cordesville, SC 29434 USA. EM damatya@fs.fed.us FU South Carolina Department of Health and Environmental Control's (DHEC) 319 [EQ-7-514]; U.S. Forest Service Southern Research Station [4OFY06, SRS 06-CO-11330135-122] FX This work was made possible by support from the South Carolina Department of Health and Environmental Control's (DHEC) 319 Grant Agreement No. EQ-7-514 (Project No. 4OFY06) with the U.S. Forest Service Southern Research Station (SRS 06-CO-11330135-122), which provided support to the Belle W. Baruch Institute of Coastal Ecology and Forest Science at Clemson University for conducting this study. The Santee Cooper Biological and Analytical Laboratory generously provided support for analyses and also important sampling and monitoring guidance throughout the project. South Carolina DHEC staff also provided ample guidance on the project. The authors would also like to acknowledge Liz Mihalik (former College of Charleston graduate student); Dr. Norm Levine (Associate Professor, College of Charleston); the town of Santee; Santee State Park; U.S. Fish and Wildlife Service; Santee National Golf Course; Orangeburg County Soil and Water Conservation District (NRCS); South Carolina Department of Transportation (DOT); U.S. Army Corps of Engineers; Andy Harrison, Hydrologic Technician, Matt Krasowski, and Roman Powers at U.S. Forest Service; David Joyner (former College of Charleston graduate student); and Weyerhaeuser Company for their various levels of support and contribution during the project. NR 30 TC 0 Z9 0 U1 4 U2 21 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD FEB 1 PY 2014 VL 19 IS 2 BP 428 EP 438 DI 10.1061/(ASCE)HE.1943-5584.0000794 PG 11 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA AG1BK UT WOS:000335149700009 ER PT J AU Kiss, G Chen, XM Brindley, MA Campbell, P Afonso, CL Ke, ZL Holl, JM Guerrero-Ferreira, RC Byrd-Leotis, LA Steel, J Steinhauer, DA Plemper, RK Kelly, DF Spearman, PW Wright, ER AF Kiss, Gabriella Chen, Xuemin Brindley, Melinda A. Campbell, Patricia Afonso, Claudio L. Ke, Zunlong Holl, Jens M. Guerrero-Ferreira, Ricardo C. Byrd-Leotis, Lauren A. Steel, John Steinhauer, David A. Plemper, Richard K. Kelly, Deborah F. Spearman, Paul W. Wright, Elizabeth R. TI Capturing Enveloped Viruses on Affinity Grids for Downstream Cryo- Electron Microscopy Applications SO MICROSCOPY AND MICROANALYSIS LA English DT Article DE cryo-electron microscopy (cryo-EM); cryo-electron tomography (cryo-ET); affinity grid (AG); enveloped virus ID RESPIRATORY SYNCYTIAL VIRUS; INFLUENZA-A VIRUS; DEFECTIVE INTERFERING PARTICLES; ROUS-SARCOMA-VIRUS; CRYOELECTRON TOMOGRAPHY; MONOLAYER PURIFICATION; MEASLES-VIRUS; VIRIONS; CRYOTOMOGRAPHY; REVEALS AB Electron microscopy (EM), cryo-electron microscopy (cryo-EM), and cryo-electron tomography (cryo-ET) are essential techniques used for characterizing basic virus morphology and determining the three-dimensional structure of viruses. Enveloped viruses, which contain an outer lipoprotein coat, constitute the largest group of pathogenic viruses to humans. The purification of enveloped viruses from cell culture presents certain challenges. Specifically, the inclusion of host-membrane-derived vesicles, the complete destruction of the viruses, and the disruption of the internal architecture of individual virus particles. Here, we present a strategy for capturing enveloped viruses on affinity grids (AG) for use in both conventional EM and cryo-EM/ET applications. We examined the utility of AG for the selective capture of human immunodeficiency virus virus-like particles, influenza A, and measles virus. We applied nickel-nitrilotriacetic acid lipid layers in combination with molecular adaptors to selectively adhere the viruses to the AG surface. This further development of the AG method may prove essential for the gentle and selective purification of enveloped viruses directly onto EM grids for ultrastructural analyses. C1 [Kiss, Gabriella; Chen, Xuemin; Holl, Jens M.; Guerrero-Ferreira, Ricardo C.; Plemper, Richard K.; Spearman, Paul W.; Wright, Elizabeth R.] Emory Univ, Sch Med, Childrens Healthcare Atlanta, Dept Pediat,Div Pediat Infect Dis, Atlanta, GA 30322 USA. [Brindley, Melinda A.; Plemper, Richard K.] Georgia State Univ, Ctr Inflammat Immun & Infect, Atlanta, GA 30303 USA. [Campbell, Patricia; Byrd-Leotis, Lauren A.; Steel, John; Steinhauer, David A.] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA. [Afonso, Claudio L.] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA. [Ke, Zunlong] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA. [Kelly, Deborah F.] Virginia Tech, Carilion Res Inst, Roanoke, VA 24016 USA. RP Wright, ER (reprint author), Emory Univ, Sch Med, Childrens Healthcare Atlanta, Dept Pediat,Div Pediat Infect Dis, Atlanta, GA 30322 USA. EM erwrigh@emory.edu OI Guerrero-Ferreira, Ricardo/0000-0002-3664-8277 FU Emory University, Children's Healthcare of Atlanta; Center for AIDS Research at Emory University [P30 AI050409]; Georgia Research Alliance; NSF [0923395]; public health service grants from the NIH/NIAID [AI101775, AI058828, AI083402]; USDA_ARS CRIS [66612-32000-064]; Centers for Excellence in Influenza Research and Surveillance (CEIRS) [HHSN266200700006C] FX The authors thank Dr. Leon De Masi for providing reagents and support during the preparation of the manuscript. We would like to thank Dr. Jason Hammonds for the anti-Env polyclonal antibody and Dr. Philip Santangelo for the anti-NDV polyclonal antibody. We also thank Ms. Jeannette Taylor and Ms. Hong Yi of the Emory University Robert P. Apkarian Integrated Electron Microscopy Core for assistance. This work was supported in part by Emory University, Children's Healthcare of Atlanta, the Center for AIDS Research at Emory University (P30 AI050409), and the Georgia Research Alliance to E. R. W.; NSF grant 0923395 to E. R. W; and public health service grants AI101775 to E. R. W, AI058828 to P. W. S., and AI083402 to R. K. P. from the NIH/NIAID; USDA_ARS CRIS project number 66612-32000-064 to C. L. A.; and Centers for Excellence in Influenza Research and Surveillance (CEIRS) contract number HHSN266200700006C to J.S. and D.A.S. NR 38 TC 1 Z9 1 U1 0 U2 4 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 1431-9276 EI 1435-8115 J9 MICROSC MICROANAL JI Microsc. microanal. PD FEB PY 2014 VL 20 IS 1 BP 164 EP 174 DI 10.1017/S1431927613013937 PG 11 WC Materials Science, Multidisciplinary; Microscopy SC Materials Science; Microscopy GA AG4GU UT WOS:000335378400020 PM 24279992 ER PT J AU Ramage, JG Prentice, KW Morse, SA Carter, AJ Datta, S Drumgoole, R Gargis, SR Griffin-Thomas, L Hastings, R Masri, HP Reed, MS Sharma, SK Singh, AK Swaney, E Swanson, T Gauthier, C Toney, D Pohl, J Shakamuri, P Stuchlik, O Elder, IA Estacio, PL Garber, EAE Hojvat, S Kellogg, RB Kovacs, G Stanker, L Weigel, L Hodge, DR Pillai, SP AF Ramage, Jason G. Prentice, Kristin Willner Morse, Stephen A. Carter, Andrew J. Datta, Shomik Drumgoole, Rahsaan Gargis, Shaw R. Griffin-Thomas, LaToya Hastings, Rebecca Masri, Heather P. Reed, Matthew S. Sharma, Shashi K. Singh, Ajay K. Swaney, Erin Swanson, Tanya Gauthier, Cheryl Toney, Denise Pohl, Jan Shakamuri, Priyanka Stuchlik, Olga Elder, Ian A. Estacio, Peter L. Garber, Eric A. E. Hojvat, Sally Kellogg, Richard B. Kovacs, Gerald Stanker, Larry Weigel, Linda Hodge, David R. Pillai, Segaran P. TI Comprehensive Laboratory Evaluation of a Specific Lateral Flow Assay for the Presumptive Identification of Abrin in Suspicious White Powders and Environmental Samples SO BIOSECURITY AND BIOTERRORISM-BIODEFENSE STRATEGY PRACTICE AND SCIENCE LA English DT Article ID RIBOSOME-INACTIVATING PROTEINS; LINKED-IMMUNOSORBENT-ASSAY; SITE-DIRECTED MUTAGENESIS; A A-CHAIN; ABRUS-PRECATORIUS; IN-VITRO; RICIN; AGGLUTININ; EXPRESSION; TOXICITY AB Abrin is a heterodimeric toxin present in the seeds of the Abrus precatorius plant. The easily obtainable seeds can yield a highly toxic product that can be used in various types of biocrimes and terrorism-related activities, including "white-powder" letters. Although the vast majority of these threats are hoaxes, the lack of rapid and reliable detection assays for abrin, such as lateral flow assays (LFAs), can be an impediment to accurate and rapid hazard assessment. One of the complicating factors associated with LFAs is the use of antibodies of poor affinity and specificity that cross-react with near neighbors or that bind to plant lectins, which are capable of nonspecifically cross-linking the capture and detector antibodies. Because of the critical need to promote public safety and public health, we conducted a comprehensive laboratory evaluation of a commercial LFA for the rapid detection of abrin. This study was conducted using comprehensive inclusivity and exclusivity panels of abrin and near-neighbor plant materials, along with panels of lectins, related proteins, white powders, and environmental background material, to determine the sensitivity, specificity, limit of detection, dynamic range, and repeatability of the assay for the specific intended use of evaluating suspicious white powders and environmental samples for the presumptive presence of abrin. C1 [Ramage, Jason G.; Prentice, Kristin Willner] Booz Allen Hamilton Inc, Mclean, VA USA. [Hodge, David R.; Pillai, Segaran P.] US Dept Homeland Secur, Chem & Biol Def Div, Sci & Technol Directorate, Washington, DC 20528 USA. [Morse, Stephen A.; Carter, Andrew J.] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA USA. [Datta, Shomik; Sharma, Shashi K.; Singh, Ajay K.; Garber, Eric A. E.] US FDA, Off Regulatory Sci, Ctr Food Safety & Appl Nutr, College Pk, MD USA. [Drumgoole, Rahsaan; Swaney, Erin] Texas Dept State Hlth Serv, Emergency Preparedness Branch, Austin, TX USA. [Gargis, Shaw R.] CDC, Div Select Agents & Toxins, Atlanta, GA 30333 USA. [Griffin-Thomas, LaToya; Masri, Heather P.; Toney, Denise] Div Consolidated Lab Serv, Richmond, VA USA. [Hastings, Rebecca; Swanson, Tanya; Gauthier, Cheryl] Massachusetts Dept Publ Hlth, State Lab Inst, Bioterrorism Response Lab, Jamaica Plain, MA USA. [Reed, Matthew S.; Shakamuri, Priyanka] CDC, Div Sci Resources, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Pohl, Jan] CDC, Biotechnol Core Facil Branch, Div Sci Resources, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Elder, Ian A.] US Dept Homeland Secur, Washington, DC USA. [Estacio, Peter L.] Lawrence Livermore Natl Lab, Livermore, CA USA. [Hojvat, Sally] US FDA, Div Microbiol Devices, Silver Spring, MD USA. [Kellogg, Richard B.; Weigel, Linda] CDC, Lab Preparedness & Response Branch, Div Preparedness & Emerging Infect, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Kovacs, Gerald] ABL Inc, Rockville, MD USA. [Stanker, Larry] USDA ARS, Foodborne Toxin Detect & Control Res Unit, Albany, CA USA. RP Pillai, SP (reprint author), US Dept Homeland Secur, Chem & Biol Def Div, Sci & Technol Directorate, Washington, DC 20528 USA. EM Segaran.Pillai@HQ.DHS.GOV NR 33 TC 6 Z9 6 U1 1 U2 9 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1538-7135 EI 1557-850X J9 BIOSECUR BIOTERROR JI Biosecur. Bioterror. PD FEB 1 PY 2014 VL 12 IS 1 BP 49 EP 62 DI 10.1089/bsp.2013.0080 PG 14 WC Public, Environmental & Occupational Health; International Relations SC Public, Environmental & Occupational Health; International Relations GA AB0EA UT WOS:000331463400006 PM 24552362 ER PT J AU Cole, D Griffin, PM Fullerton, KE Ayers, T Smith, K Ingram, LA Kissler, B Hoekstra, RM AF Cole, D. Griffin, P. M. Fullerton, K. E. Ayers, T. Smith, K. Ingram, L. A. Kissler, B. Hoekstra, R. M. TI Attributing sporadic and outbreak-associated infections to sources: blending epidemiological data SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE foodborne infections; Escherichia coli; Epidemiology ID ACTIVE SURVEILLANCE NETWORK; RISK-FACTORS; FOODNET SITES; UNITED-STATES; FRACTIONS; DEFINITION; DISEASE AB Common sources of shiga toxin-producing Escherichia coli (STEC) O157 infection have been identified by investigating outbreaks and by case-control studies of sporadic infections. We conducted an analysis to attribute STEC O157 infections ascertained in 1996 and 1999 by the Foodborne Diseases Active Surveillance Network (FoodNet) to sources. Multivariable models from two case-control studies conducted in FoodNet and outbreak investigations that occurred during the study years were used to calculate the annual number of infections attributable to six sources. Using the results of the outbreak investigations alone, 27% and 15% of infections were attributed to a source in 1996 and 1999, respectively. Combining information from both data sources, 65% of infections in 1996 and 34% of infections in 1999 were attributed. The results suggest that methods to incorporate data from multiple surveillance systems and over several years are needed to improve estimation of the number of illnesses attributable to exposure sources. C1 [Cole, D.; Griffin, P. M.; Fullerton, K. E.] Ctr Dis Control & Prevent, Enter Dis Epidemiol Branch, Div Foodborne Waterborne & Environm Dis, Atlanta, GA USA. [Ayers, T.; Hoekstra, R. M.] Ctr Dis Control & Prevent, Biostat & Informat Management Off, Div Foodborne Waterborne & Environm Dis, Atlanta, GA USA. [Smith, K.] Minnesota Dept Hlth, Foodborne Vectorborne & Zoonot Dis Unit, Acute Dis Invest & Control Sect, St Paul, MN USA. [Ingram, L. A.] Tennessee Dept Hlth, Nashville, TN USA. [Kissler, B.] US Food Safety & Inspect Serv, Appl Epidemiol Div, Off Publ Hlth Sci, USDA, Atlanta, GA USA. RP Cole, D (reprint author), Div Foodborne Waterborne & Environm Dis, Analyt Team, Enter Dis Epidemiol Branch, 1600 Clifton Rd NE,MS C-09, Atlanta, GA 30333 USA. EM dcole@cdc.gov OI Ayers, Tracy/0000-0003-4140-3263 NR 22 TC 6 Z9 6 U1 2 U2 6 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD FEB PY 2014 VL 142 IS 2 BP 295 EP 302 DI 10.1017/S0950268813000915 PG 8 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA AC4XJ UT WOS:000332524300009 PM 23611460 ER PT J AU Groffman, PM Cavender-Bares, J Bettez, ND Grove, JM Hall, SJ Heffernan, JB Hobbie, SE Larson, KL Morse, JL Neill, C Nelson, K O'Neil-Dunne, J Ogden, L Pataki, DE Polsky, C Chowdhury, RR Steele, MK AF Groffman, Peter M. Cavender-Bares, Jeannine Bettez, Neil D. Grove, J. Morgan Hall, Sharon J. Heffernan, James B. Hobbie, Sarah E. Larson, Kelli L. Morse, Jennifer L. Neill, Christopher Nelson, Kristen O'Neil-Dunne, Jarlath Ogden, Laura Pataki, Diane E. Polsky, Colin Chowdhury, Rinku Roy Steele, Meredith K. TI Ecological homogenization of urban USA SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID CARBON STORAGE; UNITED-STATES; LAND-USE; URBANIZATION; NITROGEN; ECOSYSTEMS; VEGETATION; PHOENIX; LAWN; CONSEQUENCES AB A visually apparent but scientifically untested outcome of land-use change is homogenization across urban areas, where neighborhoods in different parts of the country have similar patterns of roads, residential lots, commercial areas, and aquatic features. We hypothesize that this homogenization extends to ecological structure and also to ecosystem functions such as carbon dynamics and microclimate, with continental-scale implications. Further, we suggest that understanding urban homogenization will provide the basis for understanding the impacts of urban land-use change from local to continental scales. Here, we show how multi-scale, multi-disciplinary datasets from six metropolitan areas that cover the major climatic regions of the US (Phoenix, AZ; Miami, FL; Baltimore, MD; Boston, MA; Minneapolis-St Paul, MN; and Los Angeles, CA) can be used to determine how household and neighborhood characteristics correlate with land-management practices, land-cover composition, and landscape structure and ecosystem functions at local, regional, and continental scales. C1 [Groffman, Peter M.; Bettez, Neil D.; Morse, Jennifer L.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Cavender-Bares, Jeannine; Hobbie, Sarah E.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Grove, J. Morgan] US Forest Serv, Baltimore Field Stn, USDA, Baltimore, MD USA. [Hall, Sharon J.] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Heffernan, James B.; Steele, Meredith K.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Larson, Kelli L.] Arizona State Univ, Sch Geog Sci, Tempe, AZ USA. [Larson, Kelli L.] Arizona State Univ, Sch Urban Planning & Sustainabil, Tempe, AZ USA. [Neill, Christopher] Marine Biol Lab, Ctr Ecosyst, Woods Hole, MA 02543 USA. [Nelson, Kristen] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. [Nelson, Kristen] Univ Minnesota, Dept Fisheries Wildlife & Conservat Biol, St Paul, MN 55108 USA. [O'Neil-Dunne, Jarlath] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Spatial Anal Lab, Burlington, VT USA. [Ogden, Laura] Florida Int Univ, Dept Global & Sociocultural Studies, Miami, FL 33199 USA. [Pataki, Diane E.] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA. [Polsky, Colin] Clark Univ, Grad Sch Geog, Worcester, MA 01610 USA. [Chowdhury, Rinku Roy] Indiana Univ, Dept Geog, Bloomington, IN 47405 USA. RP Groffman, PM (reprint author), Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. EM groffmanp@caryinstitute.org RI Heffernan, James/D-1261-2010; Hall, Sharon/K-7893-2012; OI Heffernan, James/0000-0001-7641-9949; Hobbie, Sarah/0000-0001-5159-031X; Morse, Jennifer/0000-0001-8872-4940 FU MacroSystems Biology Program in the Emerging Frontiers Division of the Biological Sciences Directorate at NSF; "Ecological Homogenization of Urban America" project [EF-1065548, 1065737, 1065740, 1065741, 1065772, 1065785, 1065831, 121238320]; NSF Long Term Ecological Research Program [DEB-0423476]; Phoenix [BCS-1026865, DEB-0423704, DEB-9714833]; Plum Island (Boston) [OCE-1058747, 1238212]; Cedar Creek (Minneapolis-St Paul) [DEB-0620652]; Florida Coastal Everglades (Miami) [DBI-0620409] FX We thank the MacroSystems Biology Program in the Emerging Frontiers Division of the Biological Sciences Directorate at NSF for support. The "Ecological Homogenization of Urban America" project was supported by a series of collaborative grants from this program (EF-1065548, 1065737, 1065740, 1065741, 1065772, 1065785, 1065831, 121238320). The work arose from research funded by grants from the NSF Long Term Ecological Research Program supporting work in Baltimore (DEB-0423476), Phoenix (BCS-1026865, DEB-0423704 and DEB-9714833), Plum Island (Boston) (OCE-1058747 and 1238212), Cedar Creek (Minneapolis-St Paul) (DEB-0620652), and Florida Coastal Everglades (Miami) (DBI-0620409). B Michener, S Faulkner, S Arnott, and B Wee provided helpful comments on earlier drafts of the manuscript. For author contributions, see WebPanel 1. NR 40 TC 54 Z9 54 U1 8 U2 107 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2014 VL 12 IS 1 BP 74 EP 81 DI 10.1890/120374 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AG4DX UT WOS:000335370600010 ER PT J AU Vierling, KT Swift, CE Hudak, AT Vogeler, JC Vierling, LA AF Vierling, Kerri T. Swift, Charles E. Hudak, Andrew T. Vogeler, Jody C. Vierling, Lee A. TI How much does the time lag between wildlife field- data collection and LiDAR- data acquisition matter for studies of animal distributions? A case study using bird communities SO REMOTE SENSING LETTERS LA English DT Article ID SPECIES RICHNESS; HABITAT QUALITY; FOREST; ASSEMBLAGES; VEGETATION; USA AB Vegetation structure quantified by light detection and ranging (LiDAR) can improve understanding of wildlife occupancy and species-richness patterns. However, there is often a time lag between the collection of LiDAR data and wildlife data. We investigated whether a time lag between the LiDAR acquisition and field-data acquisition affected mapped wildlife distributions ranging from an individual species distribution to total avian species richness in a conifer forest. We collected bird and LiDAR data in 2009 across a 20,000ha forest in northern Idaho. Using the 2009 LiDAR data, we modelled the probability of occurrence for the brown creeper (Certhia americana). Using the same 2009 LiDAR data, we additionally modelled total avian species richness and richness of three different bird nesting guilds (ground/understory, mid/upper canopy and cavity). We mapped brown creeper occupancy probability and species richness using the 2009 models, and then compared these maps with maps based on the same models applied to a 2003-LiDAR dataset. A prior study identified areas harvested between 2003 and 2009. There was on average a 5% absolute decrease in mapped probabilities of brown creeper occurrence in non-harvest areas between 2003 and 2009. Species richness changed by less than one species in all cases within non-harvest areas between the 2003 and 2009 maps. Although these comparisons were statistically significant at the p<0.0001 level, it is likely that the high number of map cells (similar to 480,000) influenced this result. Similar patterns between our 2003 and 2009 maps in non-harvest areas for this suite of avian responses suggests that a 6-year difference between field-data collection and LiDAR-data collection has a minimal effect on mapped avian patterns in an undisturbed coniferous forest. However, because this is one case study in one ecosystem, additional work examining the effect of temporal lags between LiDAR and field-data collection on mapping wildlife distributions is warranted in additional ecosystems. C1 [Vierling, Kerri T.; Swift, Charles E.; Vierling, Lee A.] Univ Idaho, Moscow, ID 83843 USA. [Hudak, Andrew T.] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID USA. [Vogeler, Jody C.] Oregon State Univ, Corvallis, OR 97331 USA. RP Vierling, KT (reprint author), Univ Idaho, Moscow, ID 83843 USA. EM kerriv@uidaho.edu RI Vierling, Lee/E-6428-2010; Vierling, Kerri/N-6653-2016 OI Vierling, Lee/0000-0001-5344-1983; FU USGS Gap Analysis Program [08HQAG0123]; US Forest Service Rocky Mountain Research Station [08-JV-11221633-159] FX We thank Boaz Crees for assistance in the field, and the USGS Gap Analysis Program (agreement 08HQAG0123) and the US Forest Service Rocky Mountain Research Station for funding through Joint Venture Agreement 08-JV-11221633-159. NR 28 TC 6 Z9 6 U1 2 U2 27 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 2150-704X EI 2150-7058 J9 REMOTE SENS LETT JI Remote Sens. Lett. PD FEB 1 PY 2014 VL 5 IS 2 BP 185 EP 193 DI 10.1080/2150704X.2014.891773 PG 9 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA AE4EQ UT WOS:000333935200010 ER PT J AU Bjork, A Marsden-Haug, N Nett, RJ Kersh, GJ Nicholson, W Gibson, D Szymanski, T Emery, M Kohrs, P Woodhall, D Anderson, AD AF Bjork, Adam Marsden-Haug, Nicola Nett, Randall J. Kersh, Gilbert J. Nicholson, William Gibson, Deborah Szymanski, Tahnee Emery, Michelle Kohrs, Paul Woodhall, Dana Anderson, Alicia D. TI First Reported Multistate Human Q Fever Outbreak in the United States, 2011 SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Outbreak; Goat; Montana; Coxiella; Q fever; Washington; Abortion ID COXIELLA-BURNETII; INFECTION; SEX AB In April, 2011, the Q fever bacterium Coxiella burnetii was identified at a Washington farm where an abortion storm took place among goats. Soon after, Q fever cases were reported among visitors to the farm from Washington and Montana. A cross-sectional investigation was conducted among humans and goats associated with the index farm or with 16 other farms that purchased goats from the index farm or housed goats at the index farm for breeding purposes. Questionnaire data were analyzed, along with human and goat specimens collected for evidence of C. burnetii infection. Twenty-one persons (19%) of the 109-person cohort from Washington and Montana met the outbreak case definition of an epidemiologic link to the index farm and a C. burnetii Phase II immunoglobulin G (IgG) titer >= 1:128 by immunofluorescence assay. Seventy-one percent of cases (15 of 21) were symptomatic, compared with approximately 50% during previous Q fever outbreaks. National Q fever surveillance reports increase in frequency with age, but 29% (6 of 21) of cases during this outbreak occurred in children aged <14 years. Goat-specific Q fever risk factors included direct contact with a newborn (prevalence ratio [PR] 10.7; confidence interval [CI] 1.5, 77.4), exposure to a newborn that died (PR 5.5; CI 1.7, 18.2), exposure to a weak newborn (PR 4.4; CI 1.7, 11.6), living on a property with goats (PR 4.2; CI 1.3, 13.9), and direct contact with birth/afterbirth products (PR 2.8; CI 1.1, 6.9). Evidence of C. burnetii infection was detected in all 17 goat herds sampled (13 Washington, 3 Montana, 1 Oregon) by PCR and/or enzyme-linked immunosorbent assay. Following this investigation, Washington and Montana implemented a herd management plan to encourage best-management practices among livestock owners, reduce the potential for future outbreaks, and promote continued communication between state public health and agricultural authorities. C1 [Bjork, Adam; Kersh, Gilbert J.; Nicholson, William; Anderson, Alicia D.] Ctr Dis Control & Prevent CDC, Div Vector Borne Dis, Rickettsial Zoonoses Branch, Atlanta, GA, Rep of Georgia. [Marsden-Haug, Nicola] Washington State Dept Hlth, Shoreline, WA USA. [Nett, Randall J.] CDC, Career Epidemiol Field Officer Program, Off Publ Hlth & Preparedness, Atlanta, GA 30333 USA. [Nett, Randall J.] Montana Dept Publ Hlth & Human Serv, Publ Hlth & Safety Div, Helena, MT USA. [Gibson, Deborah] Montana Dept Publ Hlth & Human Serv, Publ Hlth Lab, Helena, MT USA. [Szymanski, Tahnee] Montana Dept Livestock, Helena, MT USA. [Emery, Michelle] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. [Kohrs, Paul] Washington State Dept Agr, Olympia, WA USA. [Woodhall, Dana] CDC, Parastit Dis Branch, Div Parasit Dis & Malaria, Atlanta, GA 30333 USA. [Bjork, Adam; Woodhall, Dana] CDC, Epidem Intelligence Serv, Atlanta, GA 30333 USA. RP Bjork, A (reprint author), Ctr Dis Control & Prevent, 1600 Clifton Rd NE,Mailstop A-04, Atlanta, GA 30333 USA. EM iyk4@cdc.gov; aha5@cdc.gov OI Bjork, Adam/0000-0001-7544-2987 NR 25 TC 8 Z9 8 U1 1 U2 10 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD FEB 1 PY 2014 VL 14 IS 2 BP 111 EP 117 DI 10.1089/vbz.2012.1202 PG 7 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA AA9UD UT WOS:000331437500004 PM 24350648 ER PT J AU Chung, SY Reed, S AF Chung, Si-Yin Reed, Shawndrika TI Reducing Food Allergy: Is There Promise for Food Applications? SO CURRENT PHARMACEUTICAL DESIGN LA English DT Article DE Food allergy; allergen; processing; modification; hypoallergenic ID HIGH HYDROSTATIC-PRESSURE; ARA H 6; PULSED ULTRAVIOLET-LIGHT; LIQUID PEANUT BUTTER; BETA-LACTOGLOBULIN; ATOPIC-DERMATITIS; SENSITIZED MICE; HEAT-TREATMENT; ALLERGENICITY; IGE AB Food allergy is on the rise and has become a growing food safety concern. The main treatment is strictly avoiding allergens in the diet. However, this is difficult to do because foods are sometimes contaminated with allergens due to processing of different foods with the same machinery. For this reason, accidental ingestion of trace amount of allergenic proteins is common. For children with severe food allergy, this could be life-threatening. Food products with reduced allergenic proteins, if developed, could be beneficial and may raise the threshold of the amount of allergenic proteins required to trigger an allergic reaction. As a result, the number of serious allergic reactions may decrease. Moreover, such less allergenic products may be useful or replace regular products in studies such as oral tolerance induction or early exposure experiments, where children with severe peanut allergy are usually excluded due to their severe intolerance. This review focuses on recent findings and progress made in approaches to reduce allergenic proteins in foods. Modifying methods may include physical and chemical treatments as well as lifestyle changes and the use of supplements. We discuss the benefits and drawbacks these methods present for production of hypoallergenic food products and food allergy prevention. C1 [Chung, Si-Yin; Reed, Shawndrika] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Chung, SY (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM siyin.chung@ars.usda.gov NR 83 TC 3 Z9 3 U1 3 U2 27 PU BENTHAM SCIENCE PUBL LTD PI SHARJAH PA EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES SN 1381-6128 EI 1873-4286 J9 CURR PHARM DESIGN JI Curr. Pharm. Design PD FEB PY 2014 VL 20 IS 6 BP 924 EP 930 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA AE9EF UT WOS:000334306800012 PM 23701557 ER PT J AU Frazier-Wood, AC Borecki, IB Feitosa, MF Hopkins, PN Smith, CE Arnett, DK AF Frazier-Wood, Alexis C. Borecki, Ingrid B. Feitosa, Mary F. Hopkins, Paul N. Smith, Caren E. Arnett, Donna K. TI Sex-Specific Associations Between Screen Time and Lipoprotein Subfractions SO INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM LA English DT Article DE sedentary time; diet; fat intake; lipoprotein size; BMI; exercise; television ID NUCLEAR-MAGNETIC-RESONANCE; LDL PARTICLE-SIZE; INSULIN-RESISTANCE; PHYSICAL INACTIVITY; SKELETAL-MUSCLE; METABOLIC SYNDROME; LIPASE ACTIVITY; SEDENTARY TIME; VIEWING TIME; MEN AB Background: Time spent in sedentary activities (such as watching television) has previously been associated with several risk factors for cardiovascular disease (CVD) such as increased low-density lipoprotein cholesterol (LDL-C). Little is known about associations with lipoprotein subfractions. Using television and computer screen time in hours per day as a measure of sedentary time, we examined the association of screen time with lipoprotein subfractions. Methods: Data were used from men and women forming the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) study population. Mixed linear models specified lipoprotein measures as the outcome, and screen time as the predictor for fourteen lipoprotein subfraction measures, and included age, smoking status, pedigree, and fat, carbohydrate daily alcohol and energy intake as covariates. Analyses were run separately for men (n = 623) and women (n = 671). A step-down Bonferroni correction was applied to results. The analysis was repeated for significant results (p < .05), additionally controlling for body mass index (BMI) and moderate and vigorous physical activity. Results: Linear models indicated that screen time was associated with five lipoprotein parameters in women: the concentration of large VLDL particles (p = .01), LDL particle number (p = .01), concentration of small LDL particles (p = .04), the concentration of large HDL particles (p = .04), and HDL diameter (p = .02). All associations remained after controlling for moderate or vigorous physical activity and BMI. Conclusions: We show that sedentary time is associated with lipoprotein measures, markers of cardiometabolic disease, independently of physical activity and BMI, in women but not men. C1 [Frazier-Wood, Alexis C.] Univ Texas Houston, Sch Publ Hlth, Div Epidemiol Genet & Environm Sci, Houston, TX 77030 USA. [Borecki, Ingrid B.; Feitosa, Mary F.] Washington Univ, Sch Med, Dept Genet, St Louis, MO USA. [Hopkins, Paul N.] Univ Utah, Dept Internal Med, Salt Lake City, UT 84112 USA. [Smith, Caren E.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Arnett, Donna K.] Univ Alabama, Dept Epidemiol, Birmingham, W Midlands, England. RP Frazier-Wood, AC (reprint author), Univ Texas Houston, Sch Publ Hlth, Div Epidemiol Genet & Environm Sci, Houston, TX 77030 USA. RI Feitosa, Mary/K-8044-2012; Wood, Lekki/B-8053-2010 OI Feitosa, Mary/0000-0002-0933-2410; Wood, Lekki/0000-0001-7616-2119 FU NHLBI [U01HL072524] FX Sources of funding: This study was funded by NHLBI grant number U01HL072524. NR 33 TC 1 Z9 1 U1 0 U2 2 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 1526-484X EI 1543-2742 J9 INT J SPORT NUTR EXE JI Int. J. Sport Nutr. Exerc. Metab. PD FEB PY 2014 VL 24 IS 1 BP 59 EP 69 DI 10.1123/ijsnem.2013-0117 PG 11 WC Nutrition & Dietetics; Sport Sciences SC Nutrition & Dietetics; Sport Sciences GA AF7TC UT WOS:000334916700007 PM 23980250 ER PT J AU Sebestyen, SD Shanley, JB Boyer, EW Kendall, C Doctor, DH AF Sebestyen, Stephen D. Shanley, James B. Boyer, Elizabeth W. Kendall, Carol Doctor, Daniel H. TI Coupled hydrological and biogeochemical processes controlling variability of nitrogen species in streamflow during autumn in an upland forest SO WATER RESOURCES RESEARCH LA English DT Article DE nitrogen deposition; autumn leaf fall; dissolved organic matter; catchment hydrology; nitrate isotopes; nitrogen transformations; in-stream nitrate retention ID DISSOLVED ORGANIC-CARBON; OXYGEN-ISOTOPE COMPOSITION; TEMPERATE FORESTS; HEADWATER STREAM; FRESH-WATER; NEW-YORK; NITRIFICATION RATES; CATSKILL MOUNTAINS; SEASONAL PATTERNS; NORTHEASTERN USA AB Autumn is a season of dynamic change in forest streams of the northeastern United States due to effects of leaf fall on both hydrology and biogeochemistry. Few studies have explored how interactions of biogeochemical transformations, various nitrogen sources, and catchment flow paths affect stream nitrogen variation during autumn. To provide more information on this critical period, we studied (1) the timing, duration, and magnitude of changes to stream nitrate, dissolved organic nitrogen (DON), and ammonium concentrations; (2) changes in nitrate sources and cycling; and (3) source areas of the landscape that most influence stream nitrogen. We collected samples at higher temporal resolution for a longer duration than typical studies of stream nitrogen during autumn. This sampling scheme encompassed the patterns and extremes that occurred during base flow and stormflow events of autumn. Base flow nitrate concentrations decreased by an order of magnitude from 5.4 to 0.7 mu mol L-1 during the week when most leaves fell from deciduous trees. Changes to rates of biogeochemical transformations during autumn base flow explained the low nitrate concentrations; in-stream transformations retained up to 72% of the nitrate that entered a stream reach. A decrease of in-stream nitrification coupled with heterotrophic nitrate cycling were primary factors in the seasonal nitrate decline. The period of low nitrate concentrations ended with a storm event in which stream nitrate concentrations increased by 25-fold. In the ensuing weeks, peak stormflow nitrate concentrations progressively decreased over closely spaced, yet similarly sized events. Most stormflow nitrate originated from nitrification in near-stream areas with occasional, large inputs of unprocessed atmospheric nitrate, which has rarely been reported for nonsnowmelt events. A maximum input of 33% unprocessed atmospheric nitrate to the stream occurred during one event. Large inputs of unprocessed atmospheric nitrate show direct and rapid effects on forest streams that may be widespread, although undocumented, throughout nitrogen-polluted temperate forests. In contrast to a week-long nitrate decline during peak autumn litterfall, base flow DON concentrations increased after leaf fall and remained high for 2 months. Dissolved organic nitrogen was hydrologically flushed to the stream from riparian soils during stormflow. In contrast to distinct seasonal changes in base flow nitrate and DON concentrations, ammonium concentrations were typically at or below the detection limit, similar to the rest of the year. Our findings reveal couplings among catchment flow paths, nutrient sources, and transformations that control seasonal extremes of stream nitrogen in forested landscapes. Key Points Stream nitrate during autumn varied with inputs of atmospheric nitrate Autumn leaf fall and stormflow events affected stream nitrate and DON dynamics Stream nitrate and DON during autumn storms originated from riparian areas not hillslopes C1 [Sebestyen, Stephen D.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN USA. [Shanley, James B.] US Geol Survey, Montpelier, VT USA. [Boyer, Elizabeth W.] Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. [Kendall, Carol] US Geol Survey, Menlo Pk, CA 94025 USA. [Doctor, Daniel H.] US Geol Survey, Reston, VA 22092 USA. RP Sebestyen, SD (reprint author), US Forest Serv, USDA, No Res Stn, Grand Rapids, MN USA. EM ssebestyen@fs.fed.us RI Sebestyen, Stephen/D-1238-2013 OI Sebestyen, Stephen/0000-0002-6315-0108 FU US EPA STAR fellowship [U-916154]; Hydrology Section of the American Geophysical Union through the Horton Research grant; Edna Bailey Sussman Fund; USDA-CSREES Award; NRC postdoctoral research fellowship; USGS Water Energy and Biogeochemical Budgets program FX This research was funded by an US EPA STAR fellowship (U-916154), the Hydrology Section of the American Geophysical Union through the Horton Research grant, and a grant from the Edna Bailey Sussman Fund to SDS; a USDA-CSREES Award to EWB; a NRC postdoctoral research fellowship to DHD; and the USGS Water Energy and Biogeochemical Budgets program. Calcium concentrations were measured by T.D. Bullen, USGS, Menlo Park, CA. We thank G.R. Aiken, B.T. Aulenbach, B.L. Bedford, R.D. Briggs, M.G. Brown, T.D. Bullen, K.E. Butler, A. Chalmers, D. Choy, S.F. Clark, C.P. Dawson, J. Denner, K.Z. Doctor, R. Germain, C.L. Goodale, H. Ingleston, D. Kabler, K.A. Kendall, P.J. McHale, S. Means, M. Miller, M.J. Mitchell, P.S. Murdoch, N. Ohte, R. Sauter, S.R. Silva, A.W. Thompson, S.D. Wankel, and M.B. Young; the WL Silver lab group (University of California, Berkeley); and the Department of Environmental Science, Policy, and Management at UC Berkeley for logistical support. We thank M.B. Adams, S. Wexler, S. Godsey, S. Inamdar, and anonymous reviewers for constructive comments on drafts of this paper. This paper has not been formally reviewed by the US EPA. Any use of trade, product or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 125 TC 10 Z9 11 U1 4 U2 55 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD FEB PY 2014 VL 50 IS 2 BP 1569 EP 1591 DI 10.1002/2013WR013670 PG 23 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA AD9DC UT WOS:000333563900046 ER PT J AU Selker, JS Sander, G Steenhuis, T Barry, DA Kustas, WP AF Selker, John S. Sander, Graham Steenhuis, Tammo Barry, D. Andrew Kustas, William P. TI Learning from the scientific legacies of W. Brutsaert and J.-Y. Parlange SO WATER RESOURCES RESEARCH LA English DT Editorial Material DE solute transport; infiltration; streamflow generation; evaporation; groundwater; erosion AB Though the essence of the scientific literature is to be a repository of unaffiliated truths, scientific advancement fundamentally stems from the insights and efforts of individuals. This dichotomy can hide exemplars for young scholars of how to contribute to scientific understanding. This section of Water Resources Research addresses eminently successful strategies for advancement of the science of hydrology by exploring the ramifications of the work from Drs. Wilfried Brutsaert and Jean-Yves Parlange, colleagues who made many of the most significant contributions to the understanding of hydrologic processes of the last 50 years. The generous scope of the special section follows the key areas of their contributions, but the content looks forward from their work. Important and novel results span solute transport, infiltration, streamflow generation, and evaporation. C1 [Selker, John S.] Oregon State Univ, Dept Biol & Ecol Engn, Corvallis, OR 97331 USA. [Sander, Graham] Univ Loughborough, Dept Civil & Bldg Engn, Loughborough, Leics, England. [Steenhuis, Tammo] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY USA. [Barry, D. Andrew] Ecole Polytech Fed Lausanne, Inst Ingn Environm, Vaud, Switzerland. [Kustas, William P.] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD USA. RP Selker, JS (reprint author), Oregon State Univ, Dept Biol & Ecol Engn, Corvallis, OR 97331 USA. EM selkerj@engr.orst.edu RI Barry, David/C-6077-2008; Kustas, William/C-2063-2015; OI Barry, David/0000-0002-8621-0425; Selker, John/0000-0001-9751-6094 NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD FEB PY 2014 VL 50 IS 2 BP 1856 EP 1857 DI 10.1002/2013WR015147 PG 2 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA AD9DC UT WOS:000333563900063 ER PT J AU Gaihre, YK Wassmann, R Tirol-Padre, A Villegas-Pangga, G Aquino, E Kimball, BA AF Kanta Gaihre, Yam Wassmann, Reiner Tirol-Padre, Agnes Villegas-Pangga, Gina Aquino, Eugenio Kimball, Bruce A. TI Seasonal assessment of greenhouse gas emissions from irrigated lowland rice fields under infrared warming SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Canopy temperature; Climate change; Greenhouse gas emissions; Infrared warming; Rice ID NITROUS-OXIDE EMISSIONS; ELEVATED CARBON-DIOXIDE; AUTOMATED CHAMBER MEASUREMENTS; METHANE EMISSION; PADDY FIELDS; CH4 EMISSION; SOIL-TEMPERATURE; ORYZA-SATIVA; WATER MANAGEMENT; TIBETAN PLATEAU AB Rice fields are considered as one of the major sources of methane (CH4), and they also emit nitrous oxide (N2O). A field experiment was conducted at the International Rice Research Institute, Philippines, in 2010-2011 using a temperature free-air controlled enhancement (T-FACE) system. Our objectives were to assess (i) the suitability of the T-FACE system for flooded rice fields and (ii) seasonal variations in greenhouse gas emissions with and without experimental warming. This observation period included one wet season (WS), one dry season (DS), and a fallow season. The experimental warming, i.e., T-FACE system, was maintained by using six infrared heaters deployed in a hexagonal pattern over each plot (7.1 m(2)). Set-point canopy temperatures of the warming treatment were 1.5 and 3.0 degrees C higher than the reference plots during daytime and nighttime, respectively. Two warming treatments (i.e., heated and reference) were arranged in a randomized complete block design with three replications. Infrared warming increased rice canopy temperature by 1.1 and 2.6 degrees C (0.4 degrees C below the targeted set-point) during daytime and nighttime, respectively. On the other hand, only a marginal (0.4-0.5 degrees C) increase was observed for both water and soil temperatures, likely because flood irrigation water flowed across the field. The warming (elevated canopy temperature) had no significant effects on CH4 or N2O emissions during the dry, wet, and fallow seasons. However, diel and seasonal variations in CH4 emissions were observed during the rice-growing and fallow periods. CH4 emissions were higher during the early afternoons, which was positively correlated with both soil and air temperatures. Similarly, CH4 emission rates increased with rice growth stage up to the reproductive stage. Moreover, cumulative CH4 emissions were 1.5 times higher in the 2011 DS than in the 2010 WS (50 and 34 g CH4 m(-2), respectively). The 2-month fallow season (late May-early July 2011) under continuous flooding emitted 51 g CH4 m(-2), which is similar to that in the 2011 DS. On the other hand, N2O emissions were not detected throughout the growing season, but an emission peak was observed after final drainage at maturity during the 2011 DS. Both rice-growing and fallow seasons were the major sources of CH4 emissions as long as the field was continuously flooded, while N2O was not detectable in continuously flooded soil. Infrared warming did not affect rice yields or yield components, probably because the general growing temperatures were near optimum, and the warming treatment was not sufficiently large to cause a significant effect. (C) 2013 Elsevier B.V. All rights reserved. C1 [Kanta Gaihre, Yam; Wassmann, Reiner; Tirol-Padre, Agnes; Aquino, Eugenio] Int Rice Res Inst, Los Banos 4030, Laguna, Philippines. [Kanta Gaihre, Yam; Villegas-Pangga, Gina] Univ Philippines Los Banos, Agr Syst Cluster, Laguna 4031, Philippines. [Kimball, Bruce A.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Kanta Gaihre, Yam] Nepal Agr Res Council, Kathmandu, Nepal. RP Gaihre, YK (reprint author), Int Fertilizer Dev Ctr IFDC, House 4B,Rd 62,Gulshan 2, Dhaka, Bangladesh. EM gaihreyam@gmail.com FU Asian Development Bank-Japan Scholarship Program (ADB-JSP) FX The authors would like to express their sincere gratitude to Asian Development Bank-Japan Scholarship Program (ADB-JSP) for providing a Ph.D. scholarship to the senior author of the paper. We thank Mr. Rodolfo de los Reyes, Mr. Enrique F. Monserrat and Feriano Javier for their assistance in field and laboratory work. NR 89 TC 10 Z9 13 U1 5 U2 61 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD FEB 1 PY 2014 VL 184 BP 88 EP 100 DI 10.1016/j.agee.2013.11.024 PG 13 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA AE5BL UT WOS:000334002700010 ER PT J AU Porensky, LM Leger, EA Davison, J Miller, WW Goergen, EM Espeland, EK Carroll-Moore, EM AF Porensky, Lauren M. Leger, Elizabeth A. Davison, Jay Miller, W. Wally Goergen, Erin M. Espeland, Erin K. Carroll-Moore, Erin M. TI Arid old-field restoration: Native perennial grasses suppress weeds and erosion, but also suppress native shrubs SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Cropland abandonment; Irrigation; Native shrub facilitation; US arid Great Basin; Walker Basin; Weed control ID WYOMING BIG SAGEBRUSH; GRASSLAND RESTORATION; POSITIVE INTERACTIONS; PLANT-COMMUNITIES; ABIOTIC STRESS; LAND-USE; SOIL; ESTABLISHMENT; FACILITATION; COMPETITION AB Rates of cropland abandonment in arid regions are increasing, and abandoned fields in such regions can have low levels of ecosystem function and biodiversity. Long-lived, drought-tolerant shrubs are dominant components of many arid ecosystems, providing multiple ecosystem services such as soil stabilization, herbaceous plant facilitation, carbon storage and wildlife habitat. On abandoned agricultural fields, shrub restoration is hindered by multiple challenges, including erosion, water stress and invasive species. We hypothesized that applying short-term irrigation and seeding native perennial grasses would facilitate native shrub establishment by reducing erosion and weed abundance. Using a blocked split-plot design, we evaluated the separate and combined impacts of short-term irrigation and perennial grass seeding on five-year restoration outcomes (including direct measurements of wind erosion) at two former agricultural fields in North America's arid Great Basin. After two years, irrigation had increased the density and biomass of seeded grasses by more than ten-fold. The combination of irrigation and seeded grasses was associated with significantly lower wind erosion, weed density and weed biomass. Three years after irrigation ended, seeded grasses remained significantly more abundant in formerly irrigated than non-irrigated plots. Formerly irrigated plots also had significantly less bare ground, annual plant cover and weed biomass than non-irrigated plots. Large plant-canopy gaps were fewer in irrigated and seeded plots. Although seeded grasses reduced erosion and invasion, they failed to facilitate native shrub establishment. Shrub cover and density were highest in plots that had been drill-seeded and irrigated, but lacked perennial grasses. Our results indicate that short-term irrigation has persistent restoration benefits, and that a tradeoff exists between the benefits and costs of seeding perennial grasses into degraded arid shrubland sites. Published by Elsevier B.V. C1 [Porensky, Lauren M.; Leger, Elizabeth A.; Miller, W. Wally; Goergen, Erin M.; Espeland, Erin K.; Carroll-Moore, Erin M.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Davison, Jay] Univ Nevada Cooperat Extens, Fallon, NV 89406 USA. RP Porensky, LM (reprint author), ARS, USDA, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. EM lauren.porensky@ars.usda.gov; eleger@cabnr.unr.edu; davisonj@UNCE.unr.edu; wilymalr@cabnr.unr.edu; erin.goergen@gmail.com; erin.espeland@ars.usda.gov; ecarrollmoore@gmail.com RI Young, Kristina/M-3069-2014 FU US Bureau of Reclamation [06FC204044] FX We thank Kevin Badik, Earl Creech, Cynthia Downs, Taraneh Emam, Akiko Endo, Sandra Li, Anthony Regalia, Cub Wolfe, and Hiro Zamma for field assistance and guidance. We are grateful to Michael Collopy for coordinating this project. We thank the VV and 5 C's Ranches for the opportunity to conduct research on their properties. This project was funded by a grant under Public Law 109-103, Section 208(a), through the US Bureau of Reclamation (Cooperative Agreement 06FC204044). NR 59 TC 7 Z9 7 U1 4 U2 48 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD FEB 1 PY 2014 VL 184 BP 135 EP 144 DI 10.1016/j.agee.2013.11.026 PG 10 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA AE5BL UT WOS:000334002700014 ER PT J AU Beier, RC Byrd, JA Kubena, LF Hume, ME McReynolds, JL Anderson, RC Nisbet, DJ AF Beier, Ross C. Byrd, J. Allen, II Kubena, Leon F. Hume, Michael E. McReynolds, Jackson L. Anderson, Robin C. Nisbet, David J. TI Evaluation of linalool, a natural antimicrobial and insecticidal essential oil from basil: Effects on poultry SO POULTRY SCIENCE LA English DT Article DE bacteria; clinical chemistry panel; insect repellant; linalool; poultry ID ANTIFUNGAL ACTIVITY; BROILER-CHICKENS; GROWING CHICKS; CONSTITUENTS; FLIES; BACTERIA; (R)-(-)-LINALOOL; (-)-LINALOOL; INHIBITION; CITRONELLA AB Linalool is a natural plant-product used in perfumes, cosmetics, and flavoring agents. Linalool has proven antimicrobial and insect-repellant properties, which indicate it might be useful for control of enteropathogens or insect pests in poultry production. However, there are no published reports that linalool may be safely administered to or tolerated by chickens. Linalool was added to the diets of day-of-hatch chicks, and they were fed linalool-supplemented diets for 3 wk. We studied the effects of linalool on serum chemistry, gross pathology, feed conversion, and relative liver weights. Linalool had a dramatic negative dose-dependent effect on feed conversion at concentrations in the feed exceeding 2% linalool, but not on gross pathology. Liver weights were significantly increased in the 5% linalool-treated birds. There was a statistical effect on blood glucose, but this parameter remained below the cut-offs for elevated serum glucose, and the result is likely of no biological significance. Linalool caused serum aspartate aminotransferase (AST) levels to increase, but it did not increase serum gamma-glutamyl transferase levels. The linalool effect on AST was dose-dependent, but in linalool doses between 0.1 and 2% of the feed, AST was not elevated beyond normal parameters. Linalool at 2% or less may be safely added to chicken feed. We suggest future studies to evaluate the addition of linalool to the litter, where it may be used as an antimicrobial or an insect repellant or to produce a calming effect. C1 [Beier, Ross C.; Byrd, J. Allen, II; Kubena, Leon F.; Hume, Michael E.; McReynolds, Jackson L.; Anderson, Robin C.; Nisbet, David J.] USDA ARS, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. RP Beier, RC (reprint author), USDA ARS, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. EM ross.beier@ars.usda.gov NR 44 TC 9 Z9 9 U1 3 U2 30 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2014 VL 93 IS 2 BP 267 EP 272 DI 10.3382/ps.2013-03254 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA AE5RD UT WOS:000334045600003 PM 24570447 ER PT J AU Webber, CL Taylor, MJ Shrefler, JW AF Webber, Charles L., III Taylor, Merritt J. Shrefler, James W. TI Weed Control in Yellow Squash Using Sequential Postdirected Applications of Pelargonic Acid SO HORTTECHNOLOGY LA English DT Article DE Amaranthus spinosus; crop injury; crop yield; Cucubita pepo; cutleaf groundcherry; Cyperus esculentus; Digitaria ischaemum; Physalis angulata; phytotoxicity; smooth crabgrass; spiny amaranth; yellow nutsedge AB Squash (Cucurbita pepo) producers could benefit from additional herbicide options that are safe to the crop and provide effective weed control. Research was conducted in southeastern Oklahoma (Atoka County, Lane, OK) during 2010 and 2011 to determine the impact of pelargonic acid (PA) on weed control efficacy, crop injury, and squash yields. The experiment included PA applied unshielded postdirected at 5, 10, and 15 lb/acre, plus an untreated weedy control and an untreated weed-free control. 'Enterprise' yellow squash was direct-seeded in single rows into raised beds. Weeds included smooth crabgrass (Digitaria ischaemum), cutleaf groundcherry (Physalis angulata), spiny amaranth (Amaranthus spinosus), and yellow nutsedge ( Cyperus esculentus). Pelargonic acid was applied each year in mid-July and then reapplied 8 days later. The maximum smooth crabgrass control (98%), broadleaf weed control (94%), and yellow nutsedge control (41%) was observed with the 15-lb/acre PA treatment at 9 days after initial spray treatment (DAIT), 1 day after sequential treatment (1 DAST). Pelargonic acid at 15 lb/acre provided equal or slightly greater smooth crabgrass and broadleaf (cutleaf groundcherry and spiny amaranth) control compared with the 10-lb/acre application, and consistently greater control than the 5-lb/acre rate and the weedy control. Pelargonic acid was less effective at controlling yellow nutsedge than smooth crabgrass and broadleaf weeds. Yellow nutsedge control peaked at 9 DAIT (1 DAST) with 10-lb/acre PA (41%). As the rate of PA increased from 5 to 15 lb/acre, yellow nutsedge control also increased significantly for all observation dates, except for 28 DAIT. Increasing the PA application rate increased the crop injury rating at 1 and 3 days after each application (1 and 3 DAIT, 1 and 3 DAST). Maximum squash injury occurred for each application rate at 9 DAIT (1 DAST) with 4.4%, 8.0%, and 12.5% injury for PA rates 5, 10, and 15 lb/acre, respectively. The 10-lb/acre PA treatment produced the highest squash yields (kilograms per hectare) and fruit number (fruit per hectare) compared with either the 5- or 15-lb/acre rates, and equivalent yields and fruit number as the hand-weeded weed-free treatment. The 10-lb/acre PA rate applied in a timely sequential application has the potential of providing good weed control with minimal crop injury resulting in yields equivalent to weed-free hand-weeding conditions. C1 [Webber, Charles L., III] ARS, Sugarcane Res Unit, USDA, Houma, LA USA. [Taylor, Merritt J.] Oklahoma State Univ, Dept Agr Econ, Durant, OK 74702 USA. [Shrefler, James W.] Oklahoma State Univ, Cooperat Extens Serv, Div Agr Sci & Nat Resources, Durant, OK 74702 USA. RP Taylor, MJ (reprint author), Oklahoma State Univ, Dept Agr Econ, 1901 South 9th St, Durant, OK 74702 USA. EM merritt.j.taylor@okstate.edu NR 9 TC 4 Z9 4 U1 2 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD FEB PY 2014 VL 24 IS 1 BP 25 EP 29 PG 5 WC Horticulture SC Agriculture GA AD7XT UT WOS:000333481500003 ER PT J AU Witcher, AL Blythe, EK Fain, GB Curry, KJ AF Witcher, Anthony L. Blythe, Eugene K. Fain, Glenn B. Curry, Kenneth J. TI Stem Cutting Propagation in Whole Pine Tree Substrates SO HORTTECHNOLOGY LA English DT Article DE alternative substrate; growing media; peatmoss; pine bark; Pinus taeda ID PHYSICAL-PROPERTIES; FERTILIZER RATE; GROWTH; GREENHOUSE; BARK; PEAT; NITROGEN; PETUNIA; MEDIA AB Wood-based substrates have been extensively evaluated for greenhouse and nursery crop production, yet these substrates have not been evaluated for propagation. The objective of this study was to evaluate processed whole loblolly pine trees (WPT) (Pinus taeda) as a rooting substrate for stem cutting propagation of a range of ornamental crops. Substrates included processed WPT, pine (Pinus sp.) bark (PB), and each mixed with equal parts (by volume) peatmoss (PM) (WPT:PM and PB:PM, respectively). Substrate physical (air space, container capacity, total porosity, bulk density, and particle size distribution) and chemical [pH and electrical conductivity (EC)] properties were determined for all substrates. Rooting percentage, total root length, total root volume, and total shoot length were evaluated for four species in 2008 and five species in 2009. Substrate air space was similar between PB and WPT in the 2008 experiment, and likewise between PB:PM and WPT:PM. In the 2009 experiment, PB and WPT had similar substrate air space. The addition of PM to PB and WPT resulted in reduced air space and increased container capacity in both experiments. The proportion of fine particles doubled for PB:PM and WPT:PM compared with PB and WPT, respectively. Substrate pH for all substrates ranged from 6.0 to 6.9 at 7 days after sticking (DAS) cuttings and 6.9 to 7.1 at 79 DAS. Substrate EC was below the acceptable range for all substrates except at 7 DAS. Rooting percentage was similar among substrates within each species in both experiments. The addition of PM resulted in significantly greater total root length for PB:PM and WPT:PM compared with PB and WPT, respectively, for five of the eight species. Shoot growth was most vigorous for PB:PM compared with the other substrates for all species. The study demonstrated a range of plant species can be propagated from stem cuttings in whole pine tree substrates alone or combined with PM. C1 [Witcher, Anthony L.] ARS, USDA, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. [Blythe, Eugene K.] Mississippi State Univ, Coastal Res & Extens Ctr, South Mississippi Branch, Expt Stn, Poplarville, MS 39470 USA. [Fain, Glenn B.] Auburn Univ, Dept Hort, Auburn, AL 36849 USA. [Curry, Kenneth J.] Univ So Mississippi, Dept Biol Sci, Hattiesburg, MS 39406 USA. RP Witcher, AL (reprint author), ARS, USDA, Thad Cochran Southern Hort Lab, 810 Hwy 26 West, Poplarville, MS 39470 USA. EM anthony.witcher@ars.usda.gov NR 24 TC 1 Z9 1 U1 2 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD FEB PY 2014 VL 24 IS 1 BP 30 EP 37 PG 8 WC Horticulture SC Agriculture GA AD7XT UT WOS:000333481500004 ER PT J AU Schreiner, RP Skinkis, PA Dreves, AJ AF Schreiner, R. Paul Skinkis, Patricia A. Dreves, Amy J. TI A Rapid Method to Assess Grape Rust Mites on Leaves and Observations from Case Studies in Western Oregon Vineyards SO HORTTECHNOLOGY LA English DT Article DE Calepitrimerus vitis; Eriophyidae; extraction method; leaf stippling; scouting technique; Vitis vinifera ID CALEPITRIMERUS-VITIS NALEPA; POPULATION-DYNAMICS; ACARI; ERIOPHYIDAE; VINIFERA; IMPACT; DAMAGE AB The grape rust mite [Calepitrimerus vitis (Acari: Eriophyidae)] is an important pest of grapevines ( Vitis sp.) in grape-growing regions around the world. A rapid method for extracting eriophyoid mites was adapted from earlier studies to provide integrated pest management (IPM) consultants and commercial growers with a practical, efficient, and reliable tool to monitor grape rust mites in vineyards and nursery stock vines. The rinse in bag (RIB) method allows quick extraction of mites from young shoots or from leaves using 35% to 70% ethanol or isopropanol in a sealable plastic bag. The RIB method recovered approximate to 85% of grape rust mites from single leaves in the first rinse. The method is useful to estimate grape rust mites on young shoots (<= 10 cm length), although recovery of grape rust mites (average ranging from 35% to 81%) was lower because of a higher density of trichomes on young shoots as compared with leaf samples. The MB method was not effective to assess grape rust mites within dormant buds, so a separate method using a blender to disrupt tissues and extract mites in alcohol was developed. The RIB method was used to determine grape rust mite abundance with leaf symptoms in commercial vineyards and nursery stock vines. The earliest visible symptom of grape rust mite damage on leaves in the summer was the development of stippling that is distinct from the type of damage caused by other grapevine pests. The stippling is described as numerous clear zones of small diameter (resembling pinholes) that are visible when a leaf is backlit. The severity of stippling was related to the number of grape rust mites present on leaves, with >600 occurring on leaves with severe stippling symptoms. In commercial vineyard case studies, the RIB method was used over two seasons and revealed that grape rust mite populations remained on leaves until postharvest, and foliar applications of wettable sulfur reduced grape rust mite populations on leaves. C1 [Schreiner, R. Paul] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Skinkis, Patricia A.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Dreves, Amy J.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. RP Schreiner, RP (reprint author), ARS, USDA, Hort Crops Res Lab, 3420 Northwest Orchard Ave, Corvallis, OR 97330 USA. EM Paul.Schreiner@ars.usda.gov NR 23 TC 1 Z9 1 U1 2 U2 11 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD FEB PY 2014 VL 24 IS 1 BP 38 EP 47 PG 10 WC Horticulture SC Agriculture GA AD7XT UT WOS:000333481500005 ER PT J AU Shellie, K Cragin, J Serpe, M AF Shellie, Krista Cragin, Jacob Serpe, Marcelo TI Performance of Alternative European Wine Grape Cultivars in Southwestern Idaho: Cold Hardiness, Berry Maturity, and Yield SO HORTTECHNOLOGY LA English DT Article DE differential thermal analysis; low temperature exotherm; phenology; viticulture; Vitis vinifera ID VITIS-VINIFERA; DORMANT; BUDS; DEACCLIMATION; VINE AB The cold tolerance, phenology, yield, and fruit maturity of alternative red- and white-skinned wine grape cultivars ( Vitis vinifera) of European origin were compared with those of 'Merlot' and 'Cabernet Sauvignon' over two growing seasons in southwestern Idaho. Variability among alternative cultivars was detected for cold hardiness, onset of phenological events, yield, and fruit composition. The red-skinned cultivars Montepulciano and Tinto Cao were the least cold hardy of the alternative cultivars. The red-skinned cultivars Aleatico, Aglianico, and Graciano had similar yield but matured later than the leading cultivars Merlot and Cabernet Sauvignon and were among the more cold-sensitive alternative cultivars. 'Gruner Veltliner', 'Sauvignon Gris', and 'Trousseau' cold acclimated early, had high midwinter bud cold hardiness, and had similar or higher yield and fruit maturity as the leading cultivars. The white-skinned cultivars Verdelho, Fernan Pires, Sauvignon Blanc Musque, and Sauvignon Gris had similar or less vine injury, similar or earlier phenology, and similar yield and fruit maturity as the leading cultivars. The large berry size of the red-skinned cultivars Trousseau and Touriga Brasiliera warrants evaluation for wine quality. The cultivar variability identified in this study for cold hardiness, phenology, cluster architecture, and yield provides a useful guide for cultivar site selection. C1 [Shellie, Krista] ARS, USDA, Hort Crops Res Unit, Parma, ID 83660 USA. [Cragin, Jacob; Serpe, Marcelo] Boise State Univ, Dept Biol Sci, Boise, ID 83725 USA. RP Shellie, K (reprint author), ARS, USDA, Hort Crops Res Unit, Worksite Parma ID,29603 U I Lane Parma, Parma, ID 83660 USA. EM Krista.Shellie@ars.usda.gov FU Viticulture Consortium West; Idaho State Department of Agriculture Specialty Crop Block Grant Program; Agricultural Research Service [5358-21000-034-00D] FX Funding for this research was acquired through grant monies from the Viticulture Consortium West, the Idaho State Department of Agriculture Specialty Crop Block Grant Program, and Agricultural Research Service project 5358-21000-034-00D entitled "Production Systems to Promote Yield and Quality of Grapes in the Pacific Northwest." The trial was established in coordination with the NE-1020 project entitled "Multi-state Evaluation of Winegrape Cultivars and Clones." We thank Alan Muir, Monte Shields, and Cheryl Franklin-Miller for their technical expertise and Winemakers LLC for the use of their field resources and materials. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Dept. of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 30 TC 1 Z9 1 U1 4 U2 11 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD FEB PY 2014 VL 24 IS 1 BP 138 EP 147 PG 10 WC Horticulture SC Agriculture GA AD7XT UT WOS:000333481500019 ER PT J AU Miralles, DG van den Berg, MJ Gash, JH Parinussa, RM de Jeu, RAM Beck, HE Holmes, TRH Jimenez, C Verhoest, NEC Dorigo, WA Teuling, AJ Dolman, AJ AF Miralles, Diego G. van den Berg, Martinus J. Gash, John H. Parinussa, Robert M. de Jeu, Richard A. M. Beck, Hylke E. Holmes, Thomas R. H. Jimenez, Carlos Verhoest, Niko E. C. Dorigo, Wouter A. Teuling, Adriaan J. Dolman, A. Johannes TI El Nino-La Nina cycle and recent trends in continental evaporation SO NATURE CLIMATE CHANGE LA English DT Article ID GLOBAL WATER CYCLE; PRECIPITATION; CLIMATE; VARIABILITY; OCEAN; INTENSIFICATION; AVHRR; MODIS AB The hydrological cycle is expected to intensify in response to global warming(1-3). Yet, little unequivocal evidence of such an acceleration has been found on a global scale(4-6). This holds in particular for terrestrial evaporation, the crucial return flow of water from land to atmosphere(7). Here we use satellite observations to reveal that continental evaporation has increased in northern latitudes, at rates consistent with expectations derived from temperature trends. However, at the global scale, the dynamics of the El Nino/Southern Oscillation (ENSO) have dominated the multi-decadal variability. During El Nino, limitations in terrestrial moisture supply result in vegetation water stress and reduced evaporation in eastern and central Australia, southern Africa and eastern South America. The opposite situation occurs during La Nina. Our results suggest that recent multi-year declines in global average continental evaporation(8,9) reflect transitions to El Nino conditions, and are not the consequence of a persistent reorganization of the terrestrial water cycle. Future changes in continental evaporation will be determined by the response of ENSO to changes in global radiative forcing, which still remains highly uncertain(10,11). C1 [Miralles, Diego G.] Univ Bristol, Sch Geog Sci, Bristol BS8 1SS, Avon, England. [van den Berg, Martinus J.; Verhoest, Niko E. C.] Univ Ghent, Lab Hydrol & Water Management, B-9000 Ghent, Belgium. [Gash, John H.; Parinussa, Robert M.; de Jeu, Richard A. M.; Beck, Hylke E.; Dolman, A. Johannes] Vrije Univ Amsterdam, Dept Earth Sci, NL-1081 HV Amsterdam, Netherlands. [Gash, John H.] Ctr Ecol & Hydrol, Wallingford OX10 8BB, Oxon, England. [Holmes, Thomas R. H.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Jimenez, Carlos] Observ Paris, CNRS, F-75014 Paris, France. [Dorigo, Wouter A.] Vienna Univ Technol, Dept Geodesy & Geoinformat, A-1040 Vienna, Austria. [Teuling, Adriaan J.] Wageningen Univ, Hydrol & Quantitat Water Management Grp, NL-6708 PA Wageningen, Netherlands. RP Miralles, DG (reprint author), Univ Bristol, Sch Geog Sci, Bristol BS8 1SS, Avon, England. EM diego.miralles@bristol.ac.uk RI Holmes, Thomas/F-4512-2010; Miralles, Diego/K-8857-2013; OI Holmes, Thomas/0000-0002-4651-0079; Miralles, Diego/0000-0001-6186-5751; Teuling, Adriaan/0000-0003-4302-2835 FU European Space Agency (ESA) WACMOS-ET project [4000106711/12/I-NB]; The Netherlands Organization for Scientific Research [016.111.002]; ESA [4000107122/12/I-NB, 4000104814/11/I-NB]; EU [282664] FX This work is financially supported by the European Space Agency (ESA) WACMOS-ET project (contract no. 4000106711/12/I-NB). A.J.T. acknowledges support from The Netherlands Organization for Scientific Research (Veni grant 016.111.002). W.A.D.'s contribution is financially supported by ESA's projects WATCHFUL (4000107122/12/I-NB) and Climate Change Initiative (4000104814/11/I-NB). A.J.D. is supported by the EU FP7 Amazalert project (grant agreement 282664). We thank B. Mueller, M. Jung and M. Reichstein for the multi-model data used in Fig. 1a. We are grateful to the research centres that made the satellite and re-analysis data available, and especially to the FLUXNET and ISMN communities for the in situ measurements used in the validations. NR 31 TC 56 Z9 59 U1 8 U2 51 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD FEB PY 2014 VL 4 IS 2 BP 122 EP 126 DI 10.1038/NCLIMATE2068 PG 5 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AE0PD UT WOS:000333667300016 ER PT J AU Nguyen, CV Vrebalov, JT Gapper, NE Zheng, Y Zhong, SL Fei, ZJ Giovannoni, JJ AF Nguyen, Cuong V. Vrebalov, Julia T. Gapper, Nigel E. Zheng, Yi Zhong, Silin Fei, Zhangjun Giovannoni, James J. TI Tomato GOLDEN2-LIKE Transcription Factors Reveal Molecular Gradients That Function during Fruit Development and Ripening SO PLANT CELL LA English DT Article ID REGULATE CHLOROPLAST DEVELOPMENT; ARABIDOPSIS GYNOECIUM; DROSOPHILA EMBRYO; GENE; PLANTS; MUTANT; PHOTOMORPHOGENESIS; BREVIPEDICELLUS; EXPRESSION; PHENOTYPE AB Fruit ripening is the summation of changes rendering fleshy fruit tissues attractive and palatable to seed dispersing organisms. For example, sugar content is influenced by plastid numbers and photosynthetic activity in unripe fruit and later by starch and sugar catabolism during ripening. Tomato fruit are sinks of photosynthate, yet unripe green fruit contribute significantly to the sugars that ultimately accumulate in the ripe fruit. Plastid numbers and chlorophyll content are influenced by numerous environmental and genetic factors and are positively correlated with photosynthesis and photosynthate accumulation. GOLDEN2-LIKE (GLK) transcription factors regulate plastid and chlorophyll levels. Tomato (Solanum lycopersicum), like most plants, contains two GLKs (i.e., GLK1 and GLK2/UNIFORM). Mutant and transgene analysis demonstrated that these genes encode functionally similar peptides, though differential expression renders GLK1 more important in leaves, while GLK2 is predominant in fruit. A latitudinal gradient of GLK2 expression influences the typical uneven coloration of green and ripe wild-type fruit. Transcriptome profiling revealed a broader fruit gene expression gradient throughout development. The gradient influenced general ripening activities beyond plastid development and was consistent with the easily observed yet poorly studied ripening gradient present in tomato and many fleshy fruits. C1 [Nguyen, Cuong V.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Nguyen, Cuong V.; Vrebalov, Julia T.; Gapper, Nigel E.; Zheng, Yi; Zhong, Silin; Fei, Zhangjun; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Zhong, Silin] Chinese Univ Hong Kong, Sch Life Sci, Sha Tin, Hong Kong, Peoples R China. [Fei, Zhangjun; Giovannoni, James J.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Giovannoni, JJ (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. EM jjg33@cornell.edu RI zhong, silin/O-7029-2015; Zheng, Yi/F-6150-2016 OI zhong, silin/0000-0002-0198-7383; Zheng, Yi/0000-0002-8042-7770 FU National Science Foundation Materials Research Science and Engineering Centers program [DMR-1120296]; National Science Foundation [DBI-0820612, IOS-0923312] FX We thank Andre Jagendorf for assistance with sugar extraction and analysis, John Grazul and the Cornell Center for Materials Research Shared Facilities (supported through the National Science Foundation Materials Research Science and Engineering Centers program, DMR-1120296) for help with transmission electron microscopy, and the Tomato Genetics Resource Center at UC Davis for kindly providing tomato seed. We thank Cornelius Barry for helpful comments, discussion, and insights. The Vietnam Education Foundation supported C.V.N., the New Zealand Foundation for Research Science and Technology supported N.E.G., and additional support was provided by Grants DBI-0820612 and IOS-0923312 from the National Science Foundation to J.J.G. NR 62 TC 20 Z9 22 U1 6 U2 95 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD FEB PY 2014 VL 26 IS 2 BP 585 EP 601 DI 10.1105/tpc.113.118794 PG 17 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA AE0WZ UT WOS:000333688300008 PM 24510723 ER PT J AU Simon, PW AF Simon, Philipp W. TI Progress Toward Increasing Intake of Dietary Nutrients from Vegetables and Fruits: The Case for a Greater Role for the Horticultural Sciences SO HORTSCIENCE LA English DT Article; Proceedings Paper CT Annual Conference of the American-Society-for-Horticultural-Science CY JUL 28-AUG 05, 2012 CL Miami, FL SP Amer Soc Hort Sci DE convenience; flavor; human health; nutrition; phytonutrients; vitamins ID DISEASE; RISK; CONSUMPTION; CROPS; WHEAT; YIELD; EAT AB Diet is implicated globally in the cause and severity of many diseases, including cancer, heart disease, and diabetes, and a large body of medical evidence indicates that consumption of healthier foods can alleviate both the incidence and severity of not only these diseases, but also obesity, which is a causal factor for many chronic diseases. The 2010 Dietary Guidelines for Americans indicated that several nutrients are underconsumed in the United States, including folate, magnesium, potassium, dietary fiber, and vitamins A, C, and K. Vegetables and fruits are major sources of several of these micronutrients and minerals as well as many other phytonutrients. Despite these health benefits, vegetable and fruit intake by U.S. consumers is significantly lower than recommended by dietary guidelines (less than 40% of recommended intake) and has not risen in the past decade. Collaborations between horticultural plant breeders, production and postharvest scientists with food scientists, nutritionists, medical scientists, marketing specialists, and social scientists are needed to develop plans and take action to stimulate increased vegetable and fruit intake. Increased intake may have a positive impact not only on the health of consumers, but would also increase the economic value of horticultural commodities and raise the profile of horticultural sciences. Horticultural approaches to address this important challenge, and opportunity, must be developed. C1 Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Simon, PW (reprint author), Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM philipp.simon@ars.usda.gov NR 38 TC 1 Z9 1 U1 0 U2 10 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2014 VL 49 IS 2 BP 112 EP 115 PG 4 WC Horticulture SC Agriculture GA AB9VD UT WOS:000332142900001 ER PT J AU Patil, BS Crosby, K Byrne, D Hirschi, K AF Patil, Bhimanagouda S. Crosby, Kevin Byrne, David Hirschi, Kendal TI The Intersection of Plant Breeding, Human Health, and Nutritional Security: Lessons Learned and Future Perspectives SO HORTSCIENCE LA English DT Article; Proceedings Paper CT Annual Conference of the American-Society-for-Horticultural-Science CY JUL 28-AUG 05, 2012 CL Miami, FL SP Amer Soc Hort Sci DE flavonoids; nutritional security; phytochemicals; sustainable ID TOTAL PHENOLIC CONTENT; PERFORMANCE LIQUID-CHROMATOGRAPHY; CANCER CELL-PROLIFERATION; BONE-MINERAL CONTENT; ANTIOXIDANT ACTIVITY; ENVIRONMENTAL VARIATION; ANTHOCYANIN CONTENT; APPLE CULTIVARS; VITAMIN-C; CHEMOPREVENTIVE PROPERTIES AB In recent years, concerns about global, sustainable, and nutritional security have gained substantial momentum propelled by rapid increases in global population and food insecurity. Historically, plant breeding has played a key role in improving crop yield to keep pace with the rising global population; however, current plant breeding efforts focusing on increasing yield may need to be realigned toward nutritional security issues. Although traits affecting yield and disease resistance remain essential, emerging research highlights the importance of nutrition, flavor, quality, and enhanced health-promoting properties in reducing food and nutritional impoverishment. We review plant breeding efforts to address nutritional impoverishment and the importance of consumer perception of flavor, nutrition, and quality. The Vegetable and Fruit Improvement Center (VFIC)'s interdisciplinary reiearch related to "Foods for Health" in pepper, cantaloupe, citrus, carrot, peach, and plum provides specific examples of improvements in vegetable and fruit quality and health-promoting properties. We discuss historical perspectives, case studies, current programs, and a future outlook on the role of plant breeding in nutritional security. Our work focuses on the nexus of plant breeding, human health, and nutritional security as a foundation for future plant improvement strategies. C1 [Patil, Bhimanagouda S.; Crosby, Kevin; Byrne, David; Hirschi, Kendal] Texas A&M Univ, Dept Hort Sci, Vegetable & Fruit Improvement Ctr, College Stn, TX 77845 USA. [Hirschi, Kendal] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Patil, BS (reprint author), Texas A&M Univ, Dept Hort Sci, Vegetable & Fruit Improvement Ctr, 1500 Res Pkwy A120, College Stn, TX 77845 USA. EM b-patil@tamu.edu NR 188 TC 4 Z9 4 U1 2 U2 37 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2014 VL 49 IS 2 BP 116 EP 127 PG 12 WC Horticulture SC Agriculture GA AB9VD UT WOS:000332142900002 ER PT J AU Farnham, MW Grusak, MA AF Farnham, Mark W. Grusak, Michael A. TI Assessing Nutritional Changes in a Vegetable Over Time: Issues and Considerations SO HORTSCIENCE LA English DT Article; Proceedings Paper CT Annual Conference of the American-Society-for-Horticultural-Science CY JUL 28-AUG 05, 2012 CL Miami, FL SP Amer Soc Hort Sci DE agronomic crops; Brassica oleracea; broccoli; horticultural crops; mineral content; phytonutrients; vegetable breeding ID BROCCOLI; ENZYMES; CROPS; GRAIN AB For many decades plant breeders have worked to improve vegetable crops for numerous economically important traits, like host plant resistance to disease, yield, and vegetable quality. Most improvements have been made with little knowledge as to how, or if, nutritional or phytonutrient concentrations might also be indirectly altered in the process. There have been some reports suggesting that concentrations of nutrients in vegetables have been reduced over time, possibly related to introductions of new cultivars. However, for most vegetables, current evidence indicating changes in nutrient concentrations, and specifically mineral concentrations, is circumstantial at best. To effectively test whether changes may have occurred over time as new cultivars replace older ones, appropriate field studies must be conducted wherein harvested produce from "old" vs. "new" crop cultivars is analyzed by appropriate methods and compared directly. Numerous considerations and issues such as 1) the set of cultivars to be used in field tests; 2) how nutritional concentration will be expressed; and 3) the evolution, history, and consumption changes of the crop under study must be addressed in making such direct comparisons and interpreting results. C1 [Farnham, Mark W.] ARS, USDA, US Vegetable Lab, Charleston, SC 29414 USA. [Grusak, Michael A.] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. RP Farnham, MW (reprint author), ARS, USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM Mark.Farnham@ars.usda.gov NR 22 TC 2 Z9 2 U1 1 U2 18 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2014 VL 49 IS 2 BP 128 EP 132 PG 5 WC Horticulture SC Agriculture GA AB9VD UT WOS:000332142900003 ER PT J AU Bell, RL AF Bell, Richard L. TI Fruit Quality of Pear Psylla-resistant Parental Germplasm SO HORTSCIENCE LA English DT Article DE Cacopsylla pyricola; germplasm resources; Hemiptera; insect resistance; Pyrus ID CULTIVARS AB Sixteen interspecific backcross hybrid selections from various breeding programs have been selected as prospective parents for breeding for resistance of European-type pears to the pear psyllids (Cacopsylla spp.). The Pyrus communis x P. pyrifolia (n = 6) backcross selections are derived mostly from NJ 1, an open-pollinated P. pyrifolia seedling, and the Pyrus communis x P. ussuriensis (n = 9) backcross selections are derived from Illinois 76, an open-pollinated P. ussuriensis seedling, and one Pyrus communis x P. ussuriensis cultivar. Ratings of psylla resistance have been based primarily on multiyear orchard observations under no-pesticide and minimal pesticide conditions. To select the best prospective parents, data on fruit quality and tree traits were analyzed. Fruit characteristics included harvest date, fruit size and shape, skin color, percentage blush, russet, overall appearance, texture (flesh fineness), texture type, juiciness, overall grittiness and grit size, flavor acceptability and type, aroma, and a quality index, which was an unweighted total of the scores for appearance, texture, grit, flavor, and aroma. For this report, comparisons were made to 'Bartlett', the most widely grown U.S. pear cultivar. Both the P. communis x P. pyrifolia and Pyrus communis x P. ussuriensis backcross hybrid groups had significantly lower quality indices than 'Bartlett', and most individual traits were similar in this respect. There were significant differences among selections for all traits as were differences between years within genotype for most traits with some exceptions. Harvest date, percentage blush, appearance, juiciness, flavor, and the quality index were relatively stable from year to year. Flesh texture type varied within each group. The P. communis x P. pyrifolia selection NJ Rock R23 T252 had the highest quality index of the selections. For eight traits, various selections ranked higher than 'Bartlett', although the differences were not significantly higher with the exception of the russet score. Five selections appear to have sufficient quality and are being used as parents. C1 ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Bell, RL (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM richard.bell@ars.usda.gov NR 12 TC 2 Z9 2 U1 1 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2014 VL 49 IS 2 BP 138 EP 140 PG 3 WC Horticulture SC Agriculture GA AB9VD UT WOS:000332142900005 ER PT J AU Yue, CY Gallardo, RK Luby, J Rihn, A McFerson, JR McCracken, V Whitaker, VM Finn, CE Hancock, JF Weebadde, C Sebolt, A Iezzoni, A AF Yue, Chengyan Gallardo, R. Karina Luby, James Rihn, Alicia McFerson, James R. McCracken, Vicki Whitaker, Vance M. Finn, Chad E. Hancock, James F. Weebadde, Cholani Sebolt, Audrey Iezzoni, Amy TI An Evaluation of US Strawberry Producers Trait Prioritization: Evidence from Audience Surveys SO HORTSCIENCE LA English DT Article DE fruit quality traits; ordered probit model; Rosaceae; supply chain ID BREEDING PROGRAM; FRUIT-QUALITY; CULTIVARS; SELECTIONS; SALINITY AB The primary goal of this research was to evaluate the relative importance of strawberry fruit quality and plant traits to strawberry producers. Previous studies focus on strawberry traits that impact postharvest quality and marketable yield; however, studies emphasizing the importance of these traits to strawberry producers are scarce. To investigate U.S. strawberry producer trait preferences, a series of audience surveys were conducted at four strawberry producer meetings across the United States. Results indicate that fruit firmness, fruit flavor, and fruit shelf life at retail were the most important fruit/plant traits to producers for a successful strawberry cultivar to possess. Growing state and producers' years involved in the decision-making process of strawberry farms impacted the relative importance of the fruit/plant traits. This study directly contributes to a larger investigation of supply chain members' trait preferences to improve the efficiency of Rosaceae fruit crop breeding programs and to increase the likelihood of new cultivar adoption. The overall project should result in a more efficient approach to new strawberry cultivar development and commercialization. C1 [Yue, Chengyan; Luby, James; Rihn, Alicia] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. [Yue, Chengyan] Univ Minnesota, Dept Appl Econ, St Paul, MN 55108 USA. [Gallardo, R. Karina] Washington State Univ, Puyallup Res & Extens Ctr, Ctr Precis Agr & Automated Syst, Sch Econ Sci, Puyallup, WA 98371 USA. [McFerson, James R.] Washington Tree Fruit Res Commiss, Wenatchee, WA 98801 USA. [McCracken, Vicki] Washington State Univ, Sch Econ Sci, Pullman, WA 99164 USA. [Whitaker, Vance M.] Univ Florida, Gulf Coast Res & Educ Ctr, Wimauma, FL 33598 USA. [Finn, Chad E.] ARS, USDA, Corvallis, OR 97330 USA. [Hancock, James F.; Weebadde, Cholani; Sebolt, Audrey; Iezzoni, Amy] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. RP Yue, CY (reprint author), Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. EM yuechy@umn.edu FU USDA's National Institute of Food and Agriculture-Specialty Crop Research Initiative project, RosBREED: Enabling marker-assisted breeding in Rosaceae [2009-51181-05808] FX This work was funded by USDA's National Institute of Food and Agriculture-Specialty Crop Research Initiative project, RosBREED: Enabling marker-assisted breeding in Rosaceae (2009-51181-05808). NR 28 TC 4 Z9 4 U1 0 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2014 VL 49 IS 2 BP 188 EP 193 PG 6 WC Horticulture SC Agriculture GA AB9VD UT WOS:000332142900013 ER PT J AU Wadl, PA Rinehart, TA Dattilo, AJ Pistrang, M Vito, LM Milstead, R Trigiano, RN AF Wadl, Phillip A. Rinehart, Timothy A. Dattilo, Adam J. Pistrang, Mark Vito, Lisa M. Milstead, Ryan Trigiano, Robert N. TI Propagation for the Conservation of Pityopsis ruthii, an Endangered Species from the Southeastern United States SO HORTSCIENCE LA English DT Article DE bonded fiber matrix; ex situ; in vitro; reintroduction; Ruth's golden aster; seed germination; tissue culture; vegetative propagation ID IN-VITRO PROPAGATION; PLANT; ASTER; MICROPROPAGATION; REINTRODUCTION; GROWTH AB Pityopsis ruthii is an endangered species endemic to the Hiwassee and Ocoee Rivers in Tennessee. As part of a recovery effort focused on P. ruthii, vegetative propagation and in vitro multiplication and seed germination techniques were developed. Plants were vegetatively propagated using greenhouse stock plants and wild-collected stems. Rooting occurred with and without auxin treatments but was greatest when 0.1% indole-3-butyric acid (IBA) talc was applied to the vegetative cuttings; rooting was lowest when flowering stems were used. Pro-Mix BX substrate provided the most consistent rooting. In vitro multiplication was accomplished by the removal of lateral shoots from in vitro-grown plants that were rooted on Murashige and Skoog (MS0) basal medium with 270 clones produced from a single individual after 4 months. Nineteen clones were transplanted and secured with bonded fiber matrix into their natural habitat and 14 survived for 1 year. To avoid genetic swamping of native populations with the introduction of large numbers of genetically identical individuals through clonal propagation, seed-based propagation efforts were explored. Open-pollinated seeds were collected, disinfested and germinated, and seedlings established on MS medium. Seeds were submersed in 70% ethanol for 1 minute and briefly flamed. Seeds were surface-sterilized in a range [10% to 50% (v/v)] Clorox (R) bleach solutions with vigorous shaking for 20 minutes, rinsed three times in sterile water, and germinated on MS0. Removal of pappus from seeds was required for successful disinfestations, but the bleach concentration was not critical. Successful propagation is a step toward the conservation and recovery of P. ruthii and should allow future reintroduction projects. C1 [Wadl, Phillip A.; Vito, Lisa M.; Milstead, Ryan; Trigiano, Robert N.] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Rinehart, Timothy A.] USDA ARS, Thad Cochran Southern Hort Res Lab, Poplarville, MS 39470 USA. [Dattilo, Adam J.] Tennessee Valley Author, Biol Compliance, Knoxville, TN 37902 USA. [Pistrang, Mark] USDA ARS, Cherokee Natl Forest, Cleveland, TN 37312 USA. RP Wadl, PA (reprint author), Univ Tennessee, Dept Entomol & Plant Pathol, 2505 EJ Chapman Dr,370 Plant Biotechnol Bldg, Knoxville, TN 37996 USA. EM pwadl@utk.edu FU U.S. Department of Agriculture [58-6404-7-213]; Tennessee Valley Authority FX This work was supported by the U.S. Department of Agriculture (Grant # 58-6404-7-213) and the Tennessee Valley Authority. NR 32 TC 3 Z9 3 U1 0 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2014 VL 49 IS 2 BP 194 EP 200 PG 7 WC Horticulture SC Agriculture GA AB9VD UT WOS:000332142900014 ER PT J AU Hale, AL Viator, RP Veremis, JC AF Hale, Anna L. Viator, Ryan P. Veremis, John C. TI Identification of freeze tolerant Saccharum spontaneum accessions through a pot-based study for use in sugarcane germplasm enhancement for adaptation to temperate climates SO BIOMASS & BIOENERGY LA English DT Article DE Biofuels; Cold tolerance; Breeding; S. spontaneum; Energycane; Sugarcane ID REGISTRATION; EXPRESSION; COLD AB Cold tolerant sugarcane (Saccharum spp.) cultivars are important in sub-tropical production areas because they can extend the growing and harvest seasons, and increase ratooning ability. Improved cold tolerance in Saccharum species has the potential to expand the range of adaptation of sugarcane, thus facilitating its use as a biofuel feedstock in non-traditional cane-growing areas. Selection for this trait is difficult in temperate regions because damaging freezes do not occur every year. The Objective of this study was to use a bioassay to identify sources of cold tolerance among Saccharum spontaneum accessions for use in future breeding. A total of 41 S. spontaneum accessions were evaluated for survivability of below ground (stubble) buds following exposure to freezing temperatures using a growth chamber. Pots containing stubble were frozen for six days at -7 degrees C, and bud germination was determined three weeks post-treatment. Accessions identified as having more ratoon cold tolerance than the most tolerant tested commercial variety (HoCP 96-540) were IND 81-144, IND 81-80, IND 81-165, and MPTH 97-216. These clones will be used in future crossing efforts, and their progeny will be evaluated for cold tolerance under natural conditions in colder climates. Published by Elsevier Ltd. C1 [Hale, Anna L.; Viator, Ryan P.; Veremis, John C.] USDA ARS, Sugarcane Res Lab, Houma, LA 70360 USA. RP Hale, AL (reprint author), USDA ARS, Sugarcane Res Lab, 5883 USDA Rd, Houma, LA 70360 USA. EM anna.hale@ars.usda.gov NR 21 TC 5 Z9 5 U1 0 U2 9 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD FEB PY 2014 VL 61 BP 53 EP 57 DI 10.1016/j.biombioe.2013.11.015 PG 5 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AC8SP UT WOS:000332805400004 ER PT J AU Tillman, PG Cottrell, TE Mizell, RF Kramer, E AF Tillman, P. G. Cottrell, T. E. Mizell, R. F., III Kramer, E. TI Effect of field edges on dispersal and distribution of colonizing stink bugs across farmscapes of the Southeast USA SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE Nezara viridula; edge effects; Chinavia hilaris; crop height; Euschistus servus ID NEZARA-VIRIDULA HEMIPTERA; HETEROPTERA-PENTATOMIDAE; EUSCHISTUS-SERVUS; IMMATURE STAGES; COTTON; CROPS; ABUNDANCE; DYNAMICS; ADJACENT; PLANT AB Stink bugs (Heteroptera: Pentatomidae), including Nezara viridula (L.), Euschistus servus (Say), and Chinavia hilaris (Say), are economic pests in farmscapes where they move within and between closely associated crop and non-crop habitats. Thus, field edges in these farmscapes include not only crop-to-crop interfaces but also those edges adjoining non-crop habitats. We examined the influence of field edges on colonization of stink bugs in southeastern USA farmscapes composed of typical combinations of corn, peanut, and cotton. For E. servus and N. viridula, egg-to-adult development and presence of both sexes on all crops indicated that the crops served as reproductive plants. Adult C. hilaris were rarely found on corn and on crops associated with it, and they were present mainly in cotton in peanut-cotton farmscapes. Mature crop height was significantly higher for corn than for cotton and significantly higher for cotton over peanut, and an edge effect in dispersal of stink bugs into a crop was detected up to 4.6, 8.2, and 14.6m from the crop-to-crop interface in corn, cotton, and peanut, respectively. These results suggest that stink bug dispersal into a crop decreases as crop height increases. The first stink bug-infested crop at the crop-to-crop interface was the most significant contributor of colonizing stink bugs to an adjacent crop. An edge effect in dispersal of stink bug adults was detected in corn next to non-woodlands and woodlands and in cotton adjacent to woodlands. Edge effects were never detected in side edges of peanut. Overall, our results indicate that both plant height and host plant suitability can influence edge-mediated dispersal of stink bugs at field edges. C1 [Tillman, P. G.] USDA ARS, Crop Protect & Management Res Lab, Tifton, GA 31793 USA. [Cottrell, T. E.] USDA ARS, Southeastern Fruit & Nut Tree Res Lab, Byron, GA 31008 USA. [Mizell, R. F., III] Univ Florida, NFREC Quincy, Quincy, FL 32351 USA. [Kramer, E.] Univ Georgia, Nat Resources Spatial Anal Lab, Coll Agr & Environm Sci, Athens, GA 30602 USA. RP Tillman, PG (reprint author), USDA ARS, Crop Protect & Management Res Lab, POB 748, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov NR 40 TC 10 Z9 10 U1 2 U2 16 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 EI 1475-2670 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD FEB PY 2014 VL 104 IS 1 BP 56 EP 64 DI 10.1017/S0007485313000497 PG 9 WC Entomology SC Entomology GA AD0UY UT WOS:000332950800007 PM 24044749 ER PT J AU Li, H Guillemaud, T French, BW Kuhlmann, U Toepfer, S AF Li, H. Guillemaud, T. French, B. W. Kuhlmann, U. Toepfer, S. TI Phenotypic trait changes in laboratory - reared colonies of the maize herbivore, Diabrotica virgifera virgifera SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE Zea mays; fitness; phenotypic traits; morphometrics; Chrysomelidae; activity; Coleoptera; generation number; western corn rootworm ID WESTERN CORN-ROOTWORM; MULTIPLE TRANSATLANTIC INTRODUCTIONS; COLEOPTERA-CHRYSOMELIDAE; GENETIC DIVERSITY; SEXUAL-DIMORPHISM; CROP-ROTATION; BODY-SIZE; SELECTION; BEHAVIOR; POPULATIONS AB The North American and European maize pest Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) was used to assess whether conditions of the natal field, subsequent laboratory rearing, or genetic population origin affect phenotypic traits of fitness, activity, or morphometrics. Standardized laboratory bioassays with large sample sizes revealed that none of the 16 tested traits, except crawling behaviours, appeared consistently stable across all seven tested colonies. Environmental conditions in the natal field of the F (0) generation affected trait averages of the subsequently reared F (1) generation in laboratory in ca. 47% of cases, and trait variability in 67% of cases. This was apparent for fitness and morphometrics, but less obvious for activity traits. Early generation laboratory rearing affected trait averages in ca. 56% of cases: morphometrics changed; fecundity and egg survival increased from F (1) to F (2). Trait variability increased or decreased in 38% of cases. Laboratory rearing for over more than 190 generations affected the trait averages in 60% of cases, reflected by decreases in flight activity and increases in body size, weight, and fecundity to some extent. It had little effect on trait variability, especially so for morphometric variability. The genetic population origin affected average levels of 55% and variability of 63% of phenotypic traits. A comparison among D. v. virgifera studies might be difficult if they use different populations or laboratory colonies. It is advised to consider possible effects of original field conditions, laboratory rearing, and population genetics when planning comparative studies targeting fitness, activity, or morphometric questions regarding Diabrotica species. C1 [Li, H.] Chinese Minist Agr, CABI Joint Lab Biosafety, Yuanmingyuan Western Rd 2, CN-100193 Beijing, Peoples R China. [Li, H.; Kuhlmann, U.; Toepfer, S.] CABI, CH-2800 Delemont, Switzerland. [Guillemaud, T.] INRA UMR, F-06903 Sophia Antipolis, France. [French, B. W.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Toepfer, S.] CABI, H-6800 Hodmezovasarhely, Hungary. RP Toepfer, S (reprint author), CABI, Rue Grillons 1, CH-2800 Delemont, Switzerland. EM s.toepfer@cabi.org RI guillemaud, thomas/B-4899-2012 OI guillemaud, thomas/0000-0003-0451-1644 FU French 'Agence Nationale de la Recherche' [ANR-06-BDIV-008)] FX We gratefully acknowledge Marc Ciosi and Xavier Fauvergue (INRA, Sophia Antipolis, France) for helpful comments during the planning period of experiments, and the Diabrotica team assisting Chad Nielson at USDA-ARS North Central Agricultural Research Laboratory, Brookings, South Dakota, USA for technical rearing advice and for providing USA colonies. We thank Benoit Facon (INRA, Centre de Biologie et de Gestion des Populations, Montferrier, France), Nick Mills (University of California, Berkeley, USA), Mark Szalai (University of Godollo, Hungary), Christian Lexer (University of Fribourg, Switzerland), and Gerald Bermond (INRA UMR, Sophia Antipolis, and University of Nice, France) for statistical advice. We thank Stefan Vidal (University of Goettingen, Germany) and an anonymous reviewer for their valuable comments and improvements of the manuscript. This research was funded by the French 'Agence Nationale de la Recherche' (ANR-06-BDIV-008). NR 82 TC 2 Z9 2 U1 4 U2 21 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 EI 1475-2670 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD FEB PY 2014 VL 104 IS 1 BP 97 EP 115 DI 10.1017/S000748531300059X PG 19 WC Entomology SC Entomology GA AD0UY UT WOS:000332950800011 PM 24267312 ER PT J AU Chaganti, RK Tolstykh, I Javaid, MK Neogi, T Torner, J Curtis, J Jacques, P Felson, D Lane, NE Nevitt, MC AF Chaganti, R. K. Tolstykh, I. Javaid, M. K. Neogi, T. Torner, J. Curtis, J. Jacques, P. Felson, D. Lane, N. E. Nevitt, M. C. CA Multicenter Osteoarthritis Study G TI High plasma levels of vitamin C and E are associated with incident radiographic knee osteoarthritis SO OSTEOARTHRITIS AND CARTILAGE LA English DT Article DE Knee osteoarthritis; Antioxidants; Vitamin C; Vitamin E ID ALPHA-TOCOPHEROL; LIQUID-CHROMATOGRAPHY; GAMMA-TOCOPHEROL; ASCORBIC-ACID; RISK-FACTORS; DOUBLE-BLIND; JOINT-SPACE; SERUM; CARTILAGE; PROGRESSION AB Background: Previous studies suggest that the antioxidants vitamins C and E may protect against development of knee osteoarthritis (OA). We examined the association of circulating levels of vitamin C and E with incident whole knee radiographic OA (WKROA). Methods: We performed a nested case control study of incident WKROA in MOST, a cohort of 3,026 men and women aged 50-79 years with, or at high risk of, knee OA. Incident cases were knees without either tibiofemoral (TF) or patellofemoral (PF) OA at baseline that developed TF and/or PF OA by 30-month follow-up. Two control knees per case were selected from those eligible for WKROA that did not develop it. Vitamin C and E (alpha-tocopherol) assays were done on baseline supernatant plasma (PCA) and serum samples, respectively. We examined the association of gender-specific tertiles of vitamin C and E with incident WKROA using logistic regression with GEE, adjusting for age, gender, and obesity. Results: Subjects without WKROA at baseline who were in the highest fertile of vitamin C had a higher incidence of WKROA [adjusted OR = 2.20 (95% CI: 1.12-433); P-value = 0.021], with similar results for the highest tertile of vitamin E [adjusted OR = 1.89 (1.02-3.50); P-value = 0.042], compared to those in the lowest tertiles. P-values for the trend of vitamin C and E tertiles and incident WKROA were 0.019 and 0.030, respectively. Conclusions: Higher levels of circulating vitamin C and E did not provide protection against incident radiographic knee OA, and may be associated with an increased risk of knee OA. 2013 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved. C1 [Chaganti, R. K.] Univ Calif San Francisco, Div Rheumatol, San Francisco, CA USA. [Javaid, M. K.] Univ Oxford, NIHR Musculoskeletal BRU, Nuffield Dept Orthopaed Rheumatol & Musculoskelet, Oxford OX1 2JD, England. [Neogi, T.; Felson, D.] Boston Univ, Sch Med, Clin Epidemiol Res & Training Unit, Boston, MA 02118 USA. [Torner, J.] Univ Iowa, Dept Epidemiol, Iowa City, IA USA. [Curtis, J.] Univ Alabama Birmingham, Div Clin Immunol & Rheumatol, Birmingham, AL 35294 USA. [Jacques, P.] Tufts Univ, USDA Human Nutr Res Ctr Aging, Boston, MA USA. [Lane, N. E.] Univ Calif Davis, Sch Med, Dept Med, Sacramento, CA 95817 USA. [Nevitt, M. C.] Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94107 USA. RP Nevitt, MC (reprint author), Univ Calif San Francisco, Dept Epidemiol & Biostat, 185 Berry St,Ste 5700,Lobby 5, San Francisco, CA 94107 USA. EM MNevitt@psg.ucsf.edu OI Neogi, Tuhina/0000-0002-9515-1711 FU National Institutes of Health (NIH) grants from the National Institute on Aging [U01-AG-18832, U01-AG-19069, U01-AG18820, U01-AG18947]; NIH [K23AR055127] FX The MOST Study is supported by National Institutes of Health (NIH) grants from the National Institute on Aging to Drs. Torner (U01-AG-18832), Nevitt (U01-AG-19069) and Felson (U01-AG18820) and Dr. Beth Lewis (UAB) (U01-AG18947). Dr. Neogi is supported by an NIH grant K23AR055127. NR 39 TC 7 Z9 8 U1 2 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1063-4584 EI 1522-9653 J9 OSTEOARTHR CARTILAGE JI Osteoarthritis Cartilage PD FEB PY 2014 VL 22 IS 2 BP 190 EP 196 DI 10.1016/j.joca.2013.11.008 PG 7 WC Orthopedics; Rheumatology SC Orthopedics; Rheumatology GA AC2PY UT WOS:000332347600004 PM 24291351 ER PT J AU Robertson, LJ Sprong, H Ortega, Y van der Giessen, JWB Fayer, R AF Robertson, Lucy J. Sprong, Hein Ortega, Ynes van der Giessen, Joke W. B. Fayer, Ron TI Response to Galan-Puchades and Fuentes: Taenia asiatica: neglected - but not forgotten - and almost certainly being quietly globalised SO TRENDS IN PARASITOLOGY LA English DT Letter ID FOODBORNE PARASITES C1 [Robertson, Lucy J.] Norwegian Sch Vet Sci, Parasitol Lab, Sect Microbiol Immunol & Parasitol, Dept Food Safety & Infect Biol, N-0033 Oslo, Norway. [Sprong, Hein; van der Giessen, Joke W. B.] Natl Inst Publ Hlth & Environm, Lab Zoonoses & Environm Microbiol, NL-3720 BA Bilthoven, Netherlands. [Ortega, Ynes] Univ Georgia, Ctr Food Safety, Griffin, GA 30223 USA. [Fayer, Ron] ARS, USDA, Beltsville, MD 20705 USA. RP Robertson, LJ (reprint author), Norwegian Sch Vet Sci, Parasitol Lab, Sect Microbiol Immunol & Parasitol, Dept Food Safety & Infect Biol, POB 8146 Dep, N-0033 Oslo, Norway. EM lucy.robertson@nvh.no OI Sprong, Hein/0000-0002-0218-4320 NR 4 TC 2 Z9 2 U1 0 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1471-4922 EI 1471-5007 J9 TRENDS PARASITOL JI Trends Parasitol. PD FEB PY 2014 VL 30 IS 2 BP 56 EP 57 DI 10.1016/j.pt.2013.12.008 PG 2 WC Parasitology SC Parasitology GA AC4OZ UT WOS:000332501900003 PM 24439489 ER PT J AU Yackulic, CB Reid, J Nichols, JD Hines, JE Davis, R Forsman, E AF Yackulic, Charles B. Reid, Janice Nichols, James D. Hines, James E. Davis, Raymond Forsman, Eric TI The roles of competition and habitat in the dynamics of populations and species distributions SO ECOLOGY LA English DT Article DE competitive exclusion; extirpation; metapopulation; scale; species range dynamics; Strix ID NORTHERN SPOTTED OWLS; BARRED OWLS; FIELD EXPERIMENTS; TRENDS; RATES; COOCCURRENCE; DISTURBANCE; ATTRIBUTES; SELECTION; SYMPATRY AB The role of competition in structuring biotic communities at fine spatial scales is well known from detailed process-based studies. Our understanding of competition's importance at broader scales is less resolved and mainly based on static species distribution maps. Here, we bridge this gap by examining the joint occupancy dynamics of an invading species (Barred Owl, Strix varia) and a resident species (Northern Spotted Owl, Strix occidentalis caurina) in a 1000-km(2) study area over a 22-year period. Past studies of these competitors have focused on the dynamics of one species at a time, hindering efforts to parse out the roles of habitat and competition and to forecast the future of the resident species. In addition, while these studies accounted for the imperfect detection of the focal species, no multi-season analysis of these species has accounted for the imperfect detection of the secondary species, potentially biasing inference. We analyzed survey data using models that combine the general multistate-multi-season occupancy modeling framework with autologistic modeling, allowing us to account for important aspects of our study system. We found that local extinction probability increases for each species when the other is present; however, the effect of the invader on the resident is greater. Although the species prefer different habitats, these habitats are highly correlated at the patch scale, and the impacts of invader on the resident are greatest in patches that would otherwise be optimal. As a consequence, competition leads to a weaker relationship between habitat and Northern Spotted Owl occupancy. Colonization and extinction rates of the invader are closely related to neighborhood occupancy, and over the first half of the study the availability of colonists limited the rate of population growth. Competition is likely to exclude the resident species, both through its immediate effects on local extinction and by indirectly lowering colonization rates as Northern Spotted Owl occupancy declines. Our analysis suggests that dispersal limitation affects both the invasion dynamics and the scale at which the effects of competition are observed. We also provide predictions regarding the potential costs and benefits of managing Barred Owl populations at different target levels. C1 [Yackulic, Charles B.; Nichols, James D.; Hines, James E.] US Geol Survey, Patuxent Wildlife Res Ctr, Laurel, MD 20708 USA. [Yackulic, Charles B.] Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA. [Yackulic, Charles B.] US Geol Survey, Grand Canyon Monitoring & Res Ctr, Southwest Biol Sci Ctr, Flagstaff, AZ 86001 USA. [Reid, Janice; Davis, Raymond; Forsman, Eric] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Yackulic, CB (reprint author), US Geol Survey, Patuxent Wildlife Res Ctr, 12100 Beech Forest Rd, Laurel, MD 20708 USA. EM cyackulic@usgs.gov FU National Science Foundation [DBI-1003221] FX Richard Chandler and three anonymous reviewers provided insightful comments on earlier versions of this manuscript. This material is based upon work supported by the National Science Foundation under Award Noumber DBI-1003221. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U. S. government. NR 48 TC 28 Z9 28 U1 12 U2 107 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD FEB PY 2014 VL 95 IS 2 BP 265 EP 279 DI 10.1890/13-0012.1 PG 15 WC Ecology SC Environmental Sciences & Ecology GA AA9RB UT WOS:000331429500003 PM 24669721 ER PT J AU Leffler, AJ James, JJ Monaco, TA Sheley, RL AF Leffler, A. Joshua James, Jeremy J. Monaco, Thomas A. Sheley, Roger L. TI A new perspective on trait differences between native and invasive exotic plants SO ECOLOGY LA English DT Article DE context dependence; ecological importance; effect size; functional traits; invasion; invasive species; meta-analysis ID BIOLOGICAL INVASIONS; ALIEN PLANTS; ECOSYSTEM PROCESSES; FUNCTIONAL TRAITS; SOIL-FEEDBACKS; NITROGEN; METAANALYSIS; COMMUNITIES; RESISTANCE; DIVERSITY AB Functional differences between native and exotic species potentially constitute one factor responsible for plant invasion. Differences in trait values between native and exotic invasive species, however, should not be considered fixed and may depend on the context of the comparison. Furthermore, the magnitude of difference between native and exotic species necessary to trigger invasion is unknown. We propose a criterion that differences in trait values between a native and exotic invasive species must be greater than differences between co-occurring natives for this difference to be ecologically meaningful and a contributing factor to plant invasion. We used a meta-analysis to quantify the difference between native and exotic invasive species for various traits examined in previous studies and compared this value to differences among native species reported in the same studies. The effect size between native and exotic invasive species was similar to the effect size between co-occurring natives except for studies conducted in the field; in most instances, our criterion was not met although overall differences between native and exotic invasive species were slightly larger than differences between natives. Consequently, trait differences may be important in certain contexts, but other mechanisms of invasion are likely more important in most cases. We suggest that using trait values as predictors of invasion will be challenging. C1 [Leffler, A. Joshua; Monaco, Thomas A.] USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [James, Jeremy J.; Sheley, Roger L.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. RP Leffler, AJ (reprint author), Univ Alaska Anchorage, Dept Biol Sci, Ecosyst & Biomed Lab 120, 3151 Alumni Loop, Anchorage, AK 99508 USA. EM ajleffler@uaa.alaska.edu NR 62 TC 17 Z9 17 U1 15 U2 142 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD FEB PY 2014 VL 95 IS 2 BP 298 EP 305 DI 10.1890/13-0102.1 PG 8 WC Ecology SC Environmental Sciences & Ecology GA AA9RB UT WOS:000331429500005 PM 24669724 ER PT J AU Ortega, YK Benson, A Greene, E AF Ortega, Yvette K. Benson, Aubree Greene, Erick TI Invasive plant erodes local song diversity in a migratory passerine SO ECOLOGY LA English DT Article DE age structure; Centaurea maculosa; Centaurea stoebe; Chipping Sparrow; exotic plants; Lolo National Forest; western Montana; USA; savanna habitat; site fidelity; song diversity; song learning; Spizella passerina; spotted knapweed ID SITE FIDELITY; BIRD SONG; REPRODUCTIVE SUCCESS; PATTERNS; COMMUNITIES; SELECTION; IMPACTS AB Exotic plant invasions threaten ecosystems globally, but we still know little about the specific consequences for animals. Invasive plants can alter the quality of breeding habitat for songbirds, thereby impacting important demographic traits such as dispersal, philopatry, and age structure. These demographic effects may in turn alter song-learning conditions to affect song structure and diversity. We studied Chipping Sparrows (Spizella passerina) breeding in six savannas that were either dominated by native vegetation or invaded by spotted knapweed (Centaurea stoebe), an exotic forb known to diminish food resources and reproductive success. Here, we report that the prevalence of older birds was relatively low in knapweed-invaded habitat, where recruitment of yearlings compensated for diminished site fidelity to sustain territory abundance. In both habitat types, yearling males tended to adopt songs similar to their neighbors and match the songs of older birds rather than introducing new song types, a pattern seen in many songbird species. As a consequence, in invaded habitat where age structure was skewed away from older birds serving as potential song models, yearlings converged on fewer song types. Similarity of songs among individuals was significantly higher and the overall number of song types averaged nearly 20% lower in invaded relative to native habitat. Degradation of habitat quality generally impacts site fidelity and age ratios in migratory songbirds and hence may commonly alter song-learning conditions. Associated shifts in song attributes known to influence reproductive success could in turn enforce demographic declines driven by habitat degradation. Local song structure may serve as an important indicator of habitat quality and population status for songbirds. C1 [Ortega, Yvette K.] USDA Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Benson, Aubree] Univ Montana, Coll Forestry & Conservat, Wildlife Biol Program, Missoula, MT 59812 USA. [Greene, Erick] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Ortega, YK (reprint author), USDA Forest Serv, Rocky Mt Res Stn, 800 East Beckwith Ave, Missoula, MT 59801 USA. EM yortega@fs.fed.us FU Rocky Mountain Research Station FX We appreciate the insightful input of C. Templeton, D. Kroodma, L. Baggett, and two anonymous reviewers on drafts of the manuscript. Many thanks to A. Zmud, B. Ogle, E. Rasmussen, L. Waller, S. Fuller, and T. Rodriguez for their hard work in the field. This research was supported by the Rocky Mountain Research Station. NR 35 TC 2 Z9 2 U1 6 U2 61 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD FEB PY 2014 VL 95 IS 2 BP 458 EP 465 DI 10.1890/12-1733.1 PG 8 WC Ecology SC Environmental Sciences & Ecology GA AA9RB UT WOS:000331429500019 PM 24669738 ER PT J AU Duveneck, MJ Scheller, RM White, MA Handler, SD Ravenscroft, C AF Duveneck, Matthew J. Scheller, Robert M. White, Mark A. Handler, Stephen D. Ravenscroft, Catherine TI Climate change effects on northern Great Lake (USA) forests: A case for preserving diversity SO ECOSPHERE LA English DT Article DE biodiversity; climate change; forest management; forest simulation model; LANDIS-II; Michigan, USA; Minnesota, USA ID LANDSCAPE SIMULATION-MODEL; TREE SPECIES MIGRATION; NET PRIMARY PRODUCTION; CARBON SEQUESTRATION; ECOSYSTEM PROCESSES; HARDWOOD FORESTS; BOREAL FORESTS; UNITED-STATES; LEAF NITROGEN; PINE-BARRENS AB Under business as usual (BAU) management, stresses posed by climate change may exceed the ability of Great Lake forests to adapt. Temperature and precipitation projections in the Great Lakes region are expected to change forest tree species composition and productivity. It is unknown how a change in productivity and/or tree species diversity due to climate change will affect the relationship between diversity and productivity. We assessed how forests in two landscapes (i.e., northern lower Michigan and northeastern Minnesota, USA) would respond to climate change and explored the diversity-productivity relationship under climate change. In addition, we explored how tree species diversity varied across landscapes by soil type, climate, and management. We used a spatially dynamic forest ecosystem model, LANDIS-II, to simulate BAU forest management under three climate scenarios (current climate, low emissions, and high emissions) in each landscape. We found a positive relationship between diversity and productivity only under a high emissions future as productivity declined. Within landscapes, climate change simulations resulted in the highest diversity in the coolest climate regions and lowest diversity in the warmest climate region in Minnesota and Michigan, respectively. Simulated productivity declined in both landscapes under the high emissions climate scenario as species such as balsam fir (Abies balsamea) declined in abundance. In the Great Lakes region, a high emissions future may decrease forest productivity creating a more positive relationship between diversity and productivity. Maintaining a diversity of tree species may become increasingly important to maintain the adaptive capacity of forests. C1 [Duveneck, Matthew J.; Scheller, Robert M.] Portland State Univ, Dept Environm Sci & Management, Portland, OR 97201 USA. [White, Mark A.] Nature Conservancy, Duluth, MN 55811 USA. [Handler, Stephen D.] US Forest Serv, Northern Inst Appl Climate Sci, Houghton, MI 49931 USA. [Ravenscroft, Catherine] Syracuse Univ, Dept Biol, Syracuse, NY 13244 USA. RP Duveneck, MJ (reprint author), Portland State Univ, Dept Environm Sci & Management, Portland, OR 97201 USA. EM mduveneck@gmail.com RI Scheller, Robert/B-3135-2009 FU U.S. Fish & Wildlife Service Upper Midwest and Great Lakes Landscape Conservation Cooperative; Portland State University Department of Environmental Science and Management FX We are grateful to the members of the Portland State University Dynamic Ecosystems and Landscapes Laboratory, Anthony W. D'Amato, Michael Falkowski, Megan Creutzburg, David Neumann, Klaus Puetmann, and Christopher Webster for their generous assistance, review and advice. Additionally, we thank two anonymous reviewers for insightful comments and perspectives. This project was funded by the U.S. Fish & Wildlife Service Upper Midwest and Great Lakes Landscape Conservation Cooperative. Additional funding was provided by the Portland State University Department of Environmental Science and Management. NR 139 TC 11 Z9 11 U1 9 U2 74 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD FEB PY 2014 VL 5 IS 2 AR 23 DI 10.1890/ES13-00370.1 PG 26 WC Ecology SC Environmental Sciences & Ecology GA AC7DE UT WOS:000332686600002 ER PT J AU Baquedano, FG Liefert, WM AF Baquedano, Felix G. Liefert, William M. TI Market integration and price transmission in consumer markets of developing countries SO FOOD POLICY LA English DT Article DE Food prices; Market integration; World price transmission; Exchange rates; Agricultural trade; Single equation error correction model ID FOOD; EQUILIBRIUM; CRISIS AB World prices for agricultural commodities surged in 2006-08, and then again in 2011-12. In many developing countries, consumer prices for staple foods, such as bread and rice, mirrored these movements. This paper examines whether prices in urban consumer markets within developing countries are co-integrated with prices in world agricultural commodity markets. Using a single equation error correction model, we examine the response of consumer prices for wheat, rice, maize, and sorghum to changes in world market prices and exchange rates in urban centers of the developing world. Analyzing over 60 country/commodity pairings, we find that developing countries' consumer markets are co-integrated with world markets. Yet, we also find that the transmission of changes in both world prices and real exchange rates to domestic consumer prices is not high, and that the movement of domestic consumer prices to new equilibrium with world prices after a shock to the latter is relatively slow. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Baquedano, Felix G.; Liefert, William M.] Econ Res Serv, USDA, Washington, DC 20024 USA. RP Baquedano, FG (reprint author), Viale Terme Caracalla, I-00153 Rome, Italy. EM Felix.Baquedano@fao.org; wliefert@ers.us-da.gov NR 27 TC 12 Z9 12 U1 1 U2 24 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0306-9192 EI 1873-5657 J9 FOOD POLICY JI Food Policy PD FEB PY 2014 VL 44 BP 103 EP 114 DI 10.1016/j.foodpol.2013.11.001 PG 12 WC Agricultural Economics & Policy; Economics; Food Science & Technology; Nutrition & Dietetics SC Agriculture; Business & Economics; Food Science & Technology; Nutrition & Dietetics GA AC7YW UT WOS:000332751200011 ER PT J AU Jessing, KK Duke, SO Cedergreeen, N AF Jessing, Karina Knudsmark Duke, Stephen O. Cedergreeen, Nina TI Potential Ecological Roles of Artemisinin Produced by Artemisia annua L. SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Review DE Allelopathy; Antifeedant; Artemisinin; Ecological role; Fungicide; Sesquiterpene ID INDUCED OXIDATIVE STRESS; ESSENTIAL OIL; ANTIMALARIAL-DRUG; ANTIMICROBIAL ACTIVITY; CHEMICAL-COMPOSITION; VULGARONE-B; SESQUITERPENE LACTONES; MOLLUSCICIDAL ACTIVITY; MOLECULAR-MECHANISM; GLANDULAR TRICHOMES AB Artemisia annua L. (annual wormwood, Asteraceae) and its secondary metabolite artemisinin, a unique sesquiterpene lactone with an endoperoxide bridge, has gained much attention due to its antimalarial properties. Artemisinin has a complex structure that requires a significant amount of energy for the plant to synthesize. So, what are the benefits to A. annua of producing this unique compound, and what is the ecological role of artemisinin? This review addresses these questions, discussing evidence of the potential utility of artemisinin in protecting the plant from insects and other herbivores, as well as pathogens and competing plant species. Abiotic factors affecting the artemisinin production, as well as mechanisms of artemisinin release to the surroundings also are discussed, and new data are provided on the toxicity of artemisinin towards soil and aquatic organisms. The antifungal and antibacterial effects reported are not very pronounced. Several studies have reported that extracts of A. annua have insecticidal effects, though few studies have proven that artemisinin could be the single compound responsible for the observed effects. However, the pathogen(s) or insect(s) that may have provided the selection pressure for the evolution of artemisinin synthesis may not have been represented in the research thus far conducted. The relatively high level of phytotoxicity of artemisinin in soil indicates that plant/plant allelopathy could be a beneficial function of artemisinin to the producing plant. The release routes of artemisinin (movement from roots and wash off from leaf surfaces) from A. annua to the soil support the rationale for allelopathy. C1 [Jessing, Karina Knudsmark; Cedergreeen, Nina] Univ Copenhagen, Dept Plant & Environm Sci, DK-1871 Frederiksberg C, Denmark. [Duke, Stephen O.] Univ Mississippi, USDA, University, MS 38677 USA. RP Jessing, KK (reprint author), Univ Copenhagen, Dept Plant & Environm Sci, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. EM jessing@plen.ku.dk RI Knudsmark Sjoholm, Karina/F-7318-2014 OI Knudsmark Sjoholm, Karina/0000-0002-2964-3849 FU Faculty of LIFE Sciences, University of Copenhagen FX We thank Thomas Bucheli for constructive comments to an earlier version of the manuscript, and the Faculty of LIFE Sciences, University of Copenhagen, for funding the research. NR 118 TC 13 Z9 15 U1 7 U2 93 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD FEB PY 2014 VL 40 IS 2 BP 100 EP 117 DI 10.1007/s10886-014-0384-6 PG 18 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA AC6DE UT WOS:000332610700002 ER PT J AU Hoover, K Keena, M Nehme, M Wang, SF Meng, P Zhang, AJ AF Hoover, Kelli Keena, Melody Nehme, Maya Wang, Shifa Meng, Peter Zhang, Aijun TI Sex-Specific Trail Pheromone Mediates Complex Mate Finding Behavior in Anoplophora glabripennis SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Sex trail pheromone; Invasive species; 2-Methyldocosane; (Z)-9-Tricosene; (Z)-9-Pentacosene; (Z)-7-Pentacosene; Mate finding; Coleaptera; Cerambycidae ID ASIAN LONGHORNED BEETLE; CUTICULAR HYDROCARBONS; COLEOPTERA; CERAMBYCIDAE; DISPERSAL; IDENTIFICATION; POPULATIONS; VOLATILES; FECUNDITY; TERMITES AB Anoplophora glabripennis (Motsch.) is a polyphagous member of the Cerambycidae, and is considered, worldwide, to be one of the most serious quarantine pests of deciduous trees. We isolated four chemicals from the trail of A. glabripennis virgin and mated females that were not present in trails of mature males. These compounds were identified as 2-methyldocosane and (Z)-9-tricosene (major components), as well as (Z)-9-pentacosene and (Z)-7-pentacosene (minor components); every trail wash sample contained all four chemical components, although the amounts and ratios changed with age of the female. Males responded to the full pheromone blend, regardless of mating status, but virgin females chose the control over the pheromone, suggesting that they may use it as a spacing pheromone to avoid intraspecific competition and maximize resources. Virgin, but not mated, males also chose the major pheromone components in the absence of the minor components, over the control. Taken together, these results indicate that all four chemicals are components of the trail pheromone. The timing of production of the ratios of the pheromone blend components that produced positive responses from males coincided with the timing of sexual maturation of the female. C1 [Hoover, Kelli; Nehme, Maya; Meng, Peter] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Hoover, Kelli; Nehme, Maya; Meng, Peter] Penn State Univ, Ctr Chem Ecol, University Pk, PA 16802 USA. [Keena, Melody] US Forest Serv, USDA, No Res Stn, Hamden, CT 06514 USA. [Wang, Shifa] Nanjing Forestry Univ, Coll Chem Engn, Nanjing 210037, Jiangsu, Peoples R China. [Zhang, Aijun] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr W, Beltsville, MD 20705 USA. RP Zhang, AJ (reprint author), ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr W, Beltsville, MD 20705 USA. EM aijun.zhang@ars.usda.gov FU USDA Northeastern Area, State and Private Forestry [10-CA-11420004-316]; Alphawood Foundation; Horticultural Research Institute FX We thank J. Nie of the United States Department of Agriculture (USDA) Invasive Insect Behavior and Biocontrol Laboratory for assistance with chemical analysis and syntheses, and G. Bradford and V. Sanchez of the USDA Forest Service for assistance in rearing beetles. Funding was provided by grants to KH from the USDA Northeastern Area, State and Private Forestry (10-CA-11420004-316), the Alphawood Foundation, and the Horticultural Research Institute. NR 43 TC 12 Z9 12 U1 9 U2 37 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD FEB PY 2014 VL 40 IS 2 BP 169 EP 180 DI 10.1007/s10886-014-0385-5 PG 12 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA AC6DE UT WOS:000332610700008 PM 24510414 ER PT J AU Han, SI Chae, JH Bilyeu, K Shannon, JG Lee, JD AF Han, Sang-Ik Chae, Jong-Hyun Bilyeu, Kristin Shannon, J. Grover Lee, Jeong-Dong TI Non-destructive Determination of High Oleic Acid Content in Single Soybean Seeds by Near Infrared Reflectance Spectroscopy SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Soybean; Oleic acid; Near infrared reflectance; Single intact seed ID MUTANT ALLELES; OIL; FAD2-1A; GENES AB Soybean [Glycine max (L.) Merr] with increased oleic acid is desirable to improve oxidative stability and functionality of soybean seed oil. Recently, soybean genotypes with high oleic acid (>= 70 %) were developed by breeding programs. Efficient and effective identification of high oleic acid soybean genotypes using non-destructive near infrared reflectance (NIR) on whole seeds would greatly enhance progress in breeding programs. The objective of this study was to develop a calibration equation for NIR determination of high oleic acid from single soybean seeds. A total of 600 intact, single F-2 seeds were scanned by NIR. Spectral data were collected between 400 and 2,500 nm at 2 nm intervals. The relationship between NIR spectral patterns of each soybean seed and its oleic acid content was examined. The best predicted equations for oleic acid were selected on the basis of minimizing the standard error of cross-validation and increasing the coefficient of determination. Validation demonstrated that the equations for determining total oleic acid and over 50 % oleic acid content had high predictive ability (r(2) = 0.91 and r(2) = 0.99, respectively). To validate the newly developed equation, F-2 seeds from a different genetic background were tested. Again, high oleic acid from single soybean seeds was accurately predicted from various genetic backgrounds. Therefore, applying the calibration equations to NIR will be useful to rapidly and efficiently select high oleic acid soybean genotypes in breeding programs. C1 [Han, Sang-Ik] RDA, Dept Funct Crop, Funct Crop Resource Dev Div, Milyang 627803, South Korea. [Chae, Jong-Hyun; Lee, Jeong-Dong] Kyungpook Natl Univ, Sch Appl Biosci, Taegu 702701, South Korea. [Bilyeu, Kristin] ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. [Shannon, J. Grover] Univ Missouri, Delta Ctr, Div Plant Sci, Portageville, MO 63873 USA. RP Lee, JD (reprint author), Kyungpook Natl Univ, Sch Appl Biosci, Taegu 702701, South Korea. EM jdlee@knu.ac.kr FU "Cooperative Research Program for Agriculture Science & Technology Development" Rural Development Administration, Republic of Korea [PJ907034] FX This work was carried out with the support of the "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ907034)" Rural Development Administration, Republic of Korea. NR 23 TC 5 Z9 5 U1 2 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD FEB PY 2014 VL 91 IS 2 BP 229 EP 234 DI 10.1007/s11746-013-2369-y PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AC6WQ UT WOS:000332666800004 ER PT J AU Compton, DL Laszlo, JA Appell, M Vermillion, KE Evans, KO AF Compton, David L. Laszlo, Joseph A. Appell, Michael Vermillion, Karl E. Evans, Kervin O. TI Synthesis, Purification, and Acyl Migration Kinetics of 2-Monoricinoleoylglycerol SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Acyl migration; Castor oil; Flash chromatography; Kinetics; 2-Monoacylglycerol; Ricinoleic acid; Structured lipids ID FATTY ACID COMPOSITION; CASTOR-OIL; PHYSICAL-PROPERTIES; 2-MONOACYLGLYCEROLS; CHROMATOGRAPHY; ESTOLIDES; IDENTIFICATION; LESQUERELLA; 2-MAG; SKIN AB 2-Monoricinoleoylglycerol (2-MRG) was synthesized by the Novozym 435 catalyzed alcoholysis of castor oil in excess ethanol (1:70 mol: mol) at ambient temperature. Due to the fatty acid C12-OH group, conventional liquid-liquid extraction methods developed for less polar, non-hydroxylated 2-monoacylglycerols (2-MAG) proved inadequate for 2-MRG purification. Alternatively, 2-MRG was purified by normal-phase flash chromatography (FC) on silica gel using a binary acetone-hexane gradient mobile phase. Gram quantities of 2-MRG were isolated in 63 % yield and contained no residual diacylglycerol (DAG), which fail to separate using liquid-liquid extraction methods. The 2-MRG was obtained at similar to 90 mol% relative to 1-MRG, proving that the FC method did not appreciably catalyze acyl migration. H-1-NMR spectroscopy was used to monitor the spontaneous acyl migration of isolated 2-MRG from 20 to 80 degrees C. The relative energy of activation calculated from the Arrhenius relationship of the 2-MRG acyl migration rate constants was 82.9 kJ/mol. This was similar to two-fold higher than the theoretical Delta G(298.15) calculated from molecular modeling using density functional calculations (B3LYP/6-31 + G*) of 2-MRG, the ketal ring transition state, and 1-mono-ricinoleoylglycerol (1-MRG). The synthesis and isolation methods reported herein provide a convenient means to access useful intermediates for functionalized structured lipids. C1 [Compton, David L.; Laszlo, Joseph A.; Evans, Kervin O.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Appell, Michael] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Vermillion, Karl E.] ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Compton, DL (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM David.Compton@ars.usda.gov NR 30 TC 4 Z9 4 U1 2 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD FEB PY 2014 VL 91 IS 2 BP 271 EP 279 DI 10.1007/s11746-013-2373-2 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AC6WQ UT WOS:000332666800008 ER PT J AU Machacova, T Bartova, E Di Loria, A Sedlak, K Mariani, U Fusco, G Fulgione, D Veneziano, V Dubey, JP AF Machacova, Tereza Bartova, Eva Di Loria, Antonio Sedlak, Kamil Mariani, Ugo Fusco, Giovanna Fulgione, Domenico Veneziano, Vincenzo Dubey, Jitender P. TI Seroprevalence of Toxoplasma gondii in Donkeys (Equus asinus) in Italy SO JOURNAL OF VETERINARY MEDICAL SCIENCE LA English DT Article DE Equus asinus; Italy; risk factors; serology; toxoplasmosis ID INFECTION; EQUIDS; BRAZIL; ELISA AB Toxoplasmosis, an important zoonosis, can be transmitted by eating meat or drinking milk of animals infected with Toxoplasma gondii. Samples were collected from 238 donkeys in the year 2010 in Italy, which included 207 females and 31 males of five breeds and crossbreeds with the average age 9 years (1 month-24 years). Sera were tested for T. gondii antibodies using a latex agglutination test and the indirect fluorescent antibody test; 5 and 8% seropositivity were recorded, respectively. We found significant correlation between the presence of T. gondii antibodies and sex, age, grazing and presence of cats on the farms and their access to donkey feed. This is the first detection of T. gondii antibodies in donkeys in Italy. C1 [Machacova, Tereza; Bartova, Eva] Univ Vet & Pharmaceut Sci, Fac Vet Hyg & Ecol, Dept Biol & Wildlife Dis, Brno 61242, Czech Republic. [Di Loria, Antonio] Magna Graecia Univ Catanzaro, Dept Hlth Sci, Catanzaro, Italy. [Sedlak, Kamil] State Vet Inst Prague, Dept Virol & Serol, Prague 6, Czech Republic. [Mariani, Ugo; Fusco, Giovanna] Ist Zooprofilatt Sperimentale Mezzogiorno, Naples, Italy. [Fulgione, Domenico] Univ Naples Federico II, Dept Biol, Naples, Italy. [Veneziano, Vincenzo] Univ Naples Federico II, Dept Vet Med & Anim Prod, Naples, Italy. [Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA,BARC E, Beltsville, MD USA. RP Bartova, E (reprint author), Univ Vet & Pharmaceut Sci, Fac Vet Hyg & Ecol, Dept Biol & Wildlife Dis, Palackeho Tr 1-3, Brno 61242, Czech Republic. EM bartovae@vfu.cz OI Di Loria, Antonio/0000-0001-6947-0287 FU Ministry of Education, Youth and Sports of the Czech Republic [MSM6215712402]; IGA VFU Brno, Czech Republic [6/2012/FVHE]; Ministry of Health of the Italian Republic [IZSME 05/10 RC C71J1000012000] FX This study was funded by the grant from the Ministry of Education, Youth and Sports of the Czech Republic (MSM6215712402) from IGA VFU Brno, Czech Republic (6/2012/FVHE) and from the Ministry of Health of the Italian Republic (IZSME 05/10 RC C71J1000012000). NR 12 TC 5 Z9 5 U1 0 U2 8 PU JAPAN SOC VET SCI PI TOKYO PA UNIV TOKYO, 1-1-1 YAYOI, BUNKYO-KU, TOKYO, 103, JAPAN SN 0916-7250 EI 1347-7439 J9 J VET MED SCI JI J. Vet. Med. Sci. PD FEB PY 2014 VL 76 IS 2 BP 265 EP 267 DI 10.1292/jvms.13-0352 PG 3 WC Veterinary Sciences SC Veterinary Sciences GA AC7OK UT WOS:000332719500017 PM 24107428 ER PT J AU Petrice, TR Haack, RA AF Petrice, Toby R. Haack, Robert A. TI Biology of the European oak borer in Michigan, United States of America, with comparisons to the native twolined chestnut borer SO CANADIAN ENTOMOLOGIST LA English DT Article ID EMERALD ASH BORER; SULCICOLLIS COLEOPTERA BUPRESTIDAE; AGRILUS-PLANIPENNIS COLEOPTERA; HYMENOPTERA-BRACONIDAE; NORTH-AMERICA; BILINEATUS; PENNSYLVANIA; FOREST; TREES AB In 2010-2011, we studied the European oak borer (EOB), Agrilus sulcicollis Lacordaire (Coleoptera: Buprestidae), in Michigan, United States of America, and made comparisons with the native twolined chestnut borer (TLCB), Agrilus bilineatus (Weber). EOB adult flight began and peaked before TLCB. More EOB females were captured on purple and white compared with yellow and green sticky traps. More TLCB females were captured on purple, followed by yellow, green, and white, respectively. Male EOB and TLCB did not show a significant colour preference. EOB completed development primarily from areas of artificially girdled trees where the phloem had died soon after trees were girdled, while TLCB usually developed in areas of girdled trees that had some live phloem present. Both EOB and TLCB successfully attacked and emerged from girdled Quercus alba Linnaeus, Quercus robur Linnaeus, and Quercus rubra Linnaeus (Fagaceae) trees. No live EOB or TLCB were found in two ungirdled and apparently healthy Q. robur trees that were dissected. EOB attacked and emerged from Q. alba, Q. robur, Q. rubra, and Quercus velutina Lamarck trap logs. Differences in the pronotal groove and terminal processes can be used to distinguish EOB from TLCB larvae. Five species of parasitoids were reared from EOB and four from TLCB. C1 [Petrice, Toby R.; Haack, Robert A.] US Forest Serv, USDA, No Res Stn, E Lansing, MI 48823 USA. RP Petrice, TR (reprint author), US Forest Serv, USDA, No Res Stn, 1407 S Harrison Rd,Rm 220, E Lansing, MI 48823 USA. EM tpetrice@fs.fed.us NR 47 TC 2 Z9 3 U1 2 U2 17 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD FEB PY 2014 VL 146 IS 1 BP 36 EP 51 DI 10.4039/tce.2013.58 PG 16 WC Entomology SC Entomology GA AC2WF UT WOS:000332376300004 ER PT J AU Dodds, KJ AF Dodds, Kevin J. TI Effects of trap height on captures of arboreal insects in pine stands of northeastern United States of America SO CANADIAN ENTOMOLOGIST LA English DT Article ID BARK BEETLES COLEOPTERA; MULTIPLE-FUNNEL; CERAMBYCID BEETLES; AMBROSIA BEETLES; PANEL TRAPS; NEW-YORK; SCOLYTIDAE; CATCHES; FOREST; PHEROMONES AB Knowledge of the effects of variables that can influence trapping results should help to optimise efforts in exotic species detection and other surveys. Two vertical trap placements (understorey, canopy) were tested to determine influence of these two heights on captures of Scolytinae (Coleoptera: Curculionidae), Cerambycidae (Coleoptera), and Siricidae (Hymenoptera) using semiochemical-baited multiple-funnel traps. Traps were baited with -pinene, ethanol, ipsdienol, and ipsenol. A total of 8463 insects from 65 species and one genus were captured during the study. Average species richness, species diversity, abundance, number of unique species, and expected diversity were higher in understorey compared with canopy traps. Jaccard (0.94 0.05) and SOrensen abundance (0.97 +/- 0.03) similarity indices suggested highly similar communities sampled at the two trap heights. Dendroctonus valens LeConte, Dryocoetes autographus Ratzeburg, Hylastes opacus Erichson, Orthotomicus caelatus (Eichhoff), Gnathotrichus materiarius (Fitch), Asemum striatum (Linnaeus), Monochamus scutellatus scutellatus (Say), Rhagium inquisitor (Linnaeus), and Xylotrechus sagitattus sagitattus (Germar) were more abundant in understorey traps. In contrast, Ips pini (Say), Pityogenes hopkinsi Swaine, Monochamus carolinensis (Olivier), Acmaeops proteus (Kirby), and Astylopsis sexgutatta (Say) were more abundant in canopy traps. The common practice of trapping in the understorey may be optimal for sampling arboreal insects as part of survey efforts. However, additional species may be found by trapping at other vertical placements. C1 US Forest Serv, USDA, Durham, NH 03824 USA. RP Dodds, KJ (reprint author), US Forest Serv, USDA, 271 Mast Rd, Durham, NH 03824 USA. EM kdodds@fs.fed.us NR 37 TC 4 Z9 4 U1 3 U2 33 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD FEB PY 2014 VL 146 IS 1 BP 80 EP 89 DI 10.4039/tce.2013.57 PG 10 WC Entomology SC Entomology GA AC2WF UT WOS:000332376300007 ER PT J AU Adrien, MD Gardner, D Pfister, J Marcolongo-Pereira, C Riet-Correa, F Schild, AL AF Adrien, Maria de Lourdes Gardner, Dale Pfister, James Marcolongo-Pereira, Clairton Riet-Correa, Franklin Schild, Ana Lucia TI Conditioned food aversion to Ipomoea carnea var. Fistulosa in sheep SO CIENCIA RURAL LA Portuguese DT Article DE food aversion; swainsonine; toxic plants; Baccharis coridifolia; sheep; Ipomoea carnea ID SUBSP FISTULOSA; CERCOCARPUS-MONTANUS; MASCAGNIA-RIGIDA; STORAGE DISEASE; TASTE-AVERSION; GOATS; CATTLE; LARKSPUR; CONVOLVULACEAE; PLANT AB Fifteen sheep were adapted to consume I. carnea for 36 days. Subsequently sheep were randomly divided into three groups of five sheep each. Group 1 was averted with LiCl, group 2 was averted with B. coridifolia, and Group 3 was the control group. The sheep were periodically tested by exposing to I. carnea in the pasture and in individual pens up to the 74th day after the first day of aversion. On the 14th and 19th days the number of sheep in Group 3 that consumed I. carnea was significantly higher than the number of sheep that consumed I. carnea in Groups 1 and 2 (P=0.004 and P=0.0004, respectively). On day 24 the number of sheep that consumed I. carnea was significantly higher in Group 3 than Group 1 (P=0.004). After the challenge on the 29th day no significant difference (P>0.05) was observed in the consumption of the plant among the three groups. On day 7 of the aversion period control sheep (group 3) consumed more I. carnea than did the sheep from Groups 1 and 2 (P= 0.0002 and P= 0.01, respectively). After this period there was no difference in the amount of I. carnea ingested by the sheep in other individual challenges. The maximum time spent by the sheep grazing I. carnea was 2.4%+/- 1.6% of the total grazing time and no difference was observed among the groups. I. carnea was never consumed avidly by the experimental sheep, and some LiCl animals did not learn to avoid the plant on a consistent basis. Conditioning an incomplete aversion indicates that neither LiCl or B. coridifolia will work with sheep in field settings to reduce or eliminate consumption of I. carnea. C1 [Adrien, Maria de Lourdes; Marcolongo-Pereira, Clairton; Schild, Ana Lucia] Univ Fed Pelotas UFPel, Fac Vet FVET, Lab Reg Diagnost, BR-96010900 Pelotas, RS, Brazil. [Gardner, Dale; Pfister, James] Utah State Univ, USDA ARS, Poisonous Plant Res Lab, Logan, UT 84322 USA. [Riet-Correa, Franklin] UFCG, Hosp Vet, CSTR, Patos de Minas, PB, Brazil. RP Schild, AL (reprint author), Univ Fed Pelotas UFPel, Fac Vet FVET, Lab Reg Diagnost, Campus Univ S-N, BR-96010900 Pelotas, RS, Brazil. EM alschild@terra.com.br RI Marcolongo-Pereira, Clairton/G-1567-2014; Schild, Ana Lucia/H-6330-2014 OI Marcolongo-Pereira, Clairton/0000-0002-5593-3110; NR 22 TC 1 Z9 1 U1 0 U2 3 PU UNIV FEDERAL SANTA MARIA PI SANTA MARIA PA UNIV FEDERAL SANTA MARIA, SANTA MARIA, RS 97105.9, BRAZIL SN 0103-8478 EI 1678-4596 J9 CIENC RURAL JI Cienc. Rural PD FEB PY 2014 VL 44 IS 2 BP 362 EP 367 PG 6 WC Agronomy SC Agriculture GA AC0QS UT WOS:000332200400027 ER PT J AU Kinziger, AP Nakamoto, RJ Harvey, BC AF Kinziger, Andrew P. Nakamoto, Rodney J. Harvey, Bret C. TI Local-scale invasion pathways and small founder numbers in introduced Sacramento pikeminnow (Ptychocheilus grandis) SO CONSERVATION GENETICS LA English DT Article DE Invasion genetics; Effective founder number; Genetic diversity; Exotic species; Sacramento pikeminnow; Ptychocheilus grandis ID MULTILOCUS GENOTYPE DATA; POPULATION-STRUCTURE; GENETIC DIVERSITY; BIOLOGICAL INVASIONS; PROPAGULE PRESSURE; SPECIES INVASIONS; EEL RIVER; NORTHWESTERN CALIFORNIA; MICROSATELLITE LOCI; RISK ANALYSIS AB Given the general pattern of invasions with severe ecological consequences commonly resulting from multiple introductions of large numbers of individuals on the intercontinental scale, we explored an example of a highly successful, ecologically significant invader introduced over a short distance, possibly via minimal propagule pressure. The Sacramento pikeminnow (Ptychocheilus grandis) has been introduced to two coastal rivers in northern California where it poses a risk to threatened and endangered fishes. We assayed variation in seven microsatellite loci and one mitochondrial DNA gene to identify the source populations and estimate founder numbers for these introductions. Our analysis suggests that successful invasion of the Eel River was likely the result of a single transfer of 3-4 effective founders from nearby within the species' native range: Clear Lake or its outflow Cache Creek. The other introduced population (Elk River), known from only seven individuals, likely represents secondary expansion from the introduced Eel River population. Our findings highlight the threat posed by close-range invaders and the ability of some fishes to rapidly invade ecologically suitable areas despite small effective founding numbers. C1 [Kinziger, Andrew P.] Humboldt State Univ, Dept Fisheries Biol, Arcata, CA 95521 USA. [Nakamoto, Rodney J.; Harvey, Bret C.] US Forest Serv, USDA, Pacific Southwest Res Stn Arcata, Arcata, CA 95521 USA. RP Kinziger, AP (reprint author), Humboldt State Univ, Dept Fisheries Biol, 1 Harpst St, Arcata, CA 95521 USA. EM andrew.kinziger@humboldt.edu NR 51 TC 1 Z9 1 U1 3 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD FEB PY 2014 VL 15 IS 1 BP 1 EP 9 DI 10.1007/s10592-013-0516-5 PG 9 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA AC2UR UT WOS:000332372300001 ER PT J AU Tucker, JM Schwartz, MK Truex, RL Wisely, SM Allendorf, FW AF Tucker, Jody M. Schwartz, Michael K. Truex, Richard L. Wisely, Samantha M. Allendorf, Fred W. TI Sampling affects the detection of genetic subdivision and conservation implications for fisher in the Sierra Nevada SO CONSERVATION GENETICS LA English DT Article DE Fisher; Pekania pennanti; Isolation by distance; Population subdivision; Sampling ID MULTILOCUS GENOTYPE DATA; POPULATION-STRUCTURE; LANDSCAPE GENETICS; MARTES-PENNANTI; MICROSATELLITE LOCI; BIOGEOGRAPHIC HISTORY; BAYESIAN METHODS; DISTANCE; CONNECTIVITY; DISPERSAL AB The small population of fisher (Pekania pennanti) in the southern Sierra Nevada is completely geographically and genetically isolated putting it at increased risk of extinction. Previous research using a clustered sampling scheme found a high amount of genetic subdivision within the southern Sierra Nevada population hypothesized to be caused by the Kings River Canyon. In this study, we use a larger and more geographically continuous set of genetic samples (n = 127) than was previously available to test this hypothesis and evaluate the genetic structure of the population. Both spatial and non-spatial population assignment models found three primary genetic clusters with moderate divergence between the clusters (F-ST = 0.05-0.13) at 10 microsatellite loci. These clusters appear to be associated with areas around the Kings River and Mountain Home State Demonstration Forest. One model also detected additional fine scale subdivision north of the Kings River that may be evidence of founder effects from a recent population expansion. The amount of population subdivision detected in this study is lower than previously found and indicates that while certain landscape features may reduce gene flow, these landscape features may be less of a barrier than initially thought. In the previous work, samples were collected in clusters which can inflate estimates of population structure by increasing the likelihood of oversampling related individuals. This study demonstrates how clustered sampling from a continuously distributed population can affect the assessment of population subdivision and influence conservation implications. C1 [Tucker, Jody M.] Sequoia Natl Forest, Porterville, CA 93257 USA. [Tucker, Jody M.] Univ Montana, Wildlife Biol Program, Missoula, MT 59812 USA. [Tucker, Jody M.; Schwartz, Michael K.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. [Truex, Richard L.] US Forest Serv, USDA, Golden, CO 80401 USA. [Wisely, Samantha M.] Univ Florida, Dept Wildlife Ecol & Conservat, Gainesville, FL USA. [Allendorf, Fred W.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Tucker, JM (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, 800 East Beckwith Ave, Missoula, MT 59801 USA. EM jtucker@fs.fed.us RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 FU USDA Forest Service Region 5; Rocky Mountain Research Station; University of Montana; National Fish and Wildlife Foundation FX We thank K. Pilgrim, C. Engkjer and P. Minton-Edison for laboratory assistance, and J. Whitfield, J. Bolis and the field crew of the Sierra Nevada Carnivore Monitoring Program. We would also like to thank M. Mitchell, M. Hebblewhite, D. Patterson, Z. Hanley and the anonymous reviewers for their comments the manuscript. This work was supported by the USDA Forest Service Region 5, the Rocky Mountain Research Station, the University of Montana, and the National Fish and Wildlife Foundation. NR 88 TC 9 Z9 9 U1 8 U2 43 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD FEB PY 2014 VL 15 IS 1 BP 123 EP 136 DI 10.1007/s10592-013-0525-4 PG 14 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA AC2UR UT WOS:000332372300010 ER PT J AU Olsoy, PJ Glenn, NF Clark, PE Derryberry, DR AF Olsoy, Peter J. Glenn, Nancy F. Clark, Patrick E. Derryberry, DeWayne R. TI Aboveground total and green biomass of dryland shrub derived from terrestrial laser scanning SO ISPRS JOURNAL OF PHOTOGRAMMETRY AND REMOTE SENSING LA English DT Article DE Terrestrial LiDAR; Sagebrush steppe; Seasonal change; Fire; Great Basin ID RABBITS BRACHYLAGUS-IDAHOENSIS; AIRBORNE LIDAR DATA; GROUND-BASED LIDAR; SAGEBRUSH STEPPE; FOOTPRINT LIDAR; FOREST STANDS; LIQUID WATER; SAGE-GROUSE; FIRE; VEGETATION AB Sagebrush (Artemisia tridentata), a dominant shrub species in the sagebrush-steppe ecosystem of the western US, is declining from its historical distribution due to feedbacks between climate and land use change, fire, and invasive species. Quantifying aboveground biomass of sagebrush is important for assessing carbon storage and monitoring the presence and distribution of this rapidly changing diyland ecosystem. Models of shrub canopy volume, derived from terrestrial laser scanning (TLS) point clouds, were used to accurately estimate aboveground sagebrush biomass. Ninety-one sagebrush plants were scanned and sampled across three study sites in the Great Basin, USA. Half of the plants were scanned and destructively sampled in the spring (n = 46), while the other half were scanned again in the fall before destructive sampling (n = 45). The latter set of sagebrush plants was scanned during both spring and fall to further test the ability of the TLS to quantify seasonal changes in green biomass. Sagebrush biomass was estimated using both a voxel and a 3-D convex hull approach applied to TLS point cloud data. The 3-D convex hull model estimated total and green biomass more accurately (R-2 = 0.92 and R-2 = 0.83, respectively) than the voxel-based method (R-2 = 0.86 and R-2 = 0.73, respectively). Seasonal differences in TLS-predicted green biomass were detected at two of the sites (p < 0.001 and p = 0.029), elucidating the amount of ephemeral leaf loss in the face of summer drought. The methods presented herein are directly transferable to other dryland shrubs, and implementation of the convex hull model with similar sagebrush species is straightforward. (C) 2013 International Society for Photogrammetry and Remote Sensing, Inc. (ISPRS) Published by Elsevier B.V. All rights reserved. C1 [Olsoy, Peter J.; Glenn, Nancy F.] Boise State Univ, Boise Ctr, Dept Geosci, Aerosp Lab, Boise, ID 83725 USA. [Clark, Patrick E.] USDA ARS, Boise, ID 83712 USA. [Derryberry, DeWayne R.] Idaho State Univ, Dept Math, Pocatello, ID 83209 USA. RP Glenn, NF (reprint author), Boise State Univ, Boise Ctr, Dept Geosci, Aerosp Lab, 1910 Univ Dr, Boise, ID 83725 USA. EM peterolsoy@boisestate.edu; nancyglenn@boisesta-te.edu; pat.clark@ars.usda.gov; derrdewa@isu.edu RI Glenn, Nancy/B-4491-2014; OI Glenn, Nancy/0000-0003-2124-7654; Clark, Patrick/0000-0003-4299-1853 FU NOAA OAR Earth Systems Research Laboratory/Physical Sciences Division (ESRL/PSD) Awards [NA09OAR4600221, NA10OAR4680240]; Idaho National Laboratory; NSF Idaho EPSCoR Program; National Science Foundation [EPS-0814387] FX Research was funded by the NOAA OAR Earth Systems Research Laboratory/Physical Sciences Division (ESRL/PSD) Awards NA09OAR4600221 & NA10OAR4680240, Idaho National Laboratory, and the NSF Idaho EPSCoR Program and by the National Science Foundation under award number EPS-0814387. We thank Mr. Randy Lee at Idaho National Laboratory for his assistance with the TLS, and Mr. Lucas Spaete, Dr. Rupesh Shrestha, Mr. Kyle Gochnour and numerous other students for providing field and lab assistance. NR 55 TC 18 Z9 18 U1 8 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0924-2716 EI 1872-8235 J9 ISPRS J PHOTOGRAMM JI ISPRS-J. Photogramm. Remote Sens. PD FEB PY 2014 VL 88 BP 166 EP 173 DI 10.1016/j.isprsjprs.2013.12.006 PG 8 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA AB6TA UT WOS:000331921200015 ER PT J AU Cox, NA Richardson, LJ Berrang, ME Rigsby, LL Buhr, RJ Plumblee, JR Fedorka-Cray, PJ AF Cox, N. A. Richardson, L. J. Berrang, M. E. Rigsby, L. L. Buhr, R. J. Plumblee, J. R. Fedorka-Cray, P. J. TI SURVIVAL OF NATURALLY OCCURRING CAMPYLOBACTER IN REFRIGERATED AND FROZEN RINSATE FROM A BROILER CARCASS - A RESEARCH NOTE SO JOURNAL OF FOOD SAFETY LA English DT Article ID JEJUNI; CHICKEN; COLI AB Each of the 10 carcasses were rinsed and tested for Campylobacter. Each rinse was subdivided into twelve 25-mL aliquots, without cryoprotectant. Six were stored at 4C and six at -23C. At 4-month intervals, one aliquot from each was streaked onto Campy-Cefex plates and then 5mL added to 45mL of Bolton's (B) or 45mL of Tecra (T) broth; after 48h at 42C, both enrichments were streaked onto Campy-Cefex plates. At 4 months (4C), 0 of 10, seven of 10 and three of 10 were positive by direct streak, B and T, respectively, while 0 of 10, one of 10 and four of 10 were positive by direct streak, B and T, respectively, at -23C. At 4 months, all 4C samples were negative, while two of 10 frozen samples were positive in both B and T after 8 months and one of 10 in B at 12, 16 and 20 months. Foods contaminated with Campylobacter may still pose a health hazard after long-term freezing. Practical ApplicationsIn this study, naturally occurring Campylobacter were able to survive in the rinsate of commercially processed broiler carcasses without the use of a cryoprotectant. The Campylobacter isolate was identified as a nalidixic acid and ciprofloxacin resistant strain of Campylobacterjejuni. Survival and periodic recovery were demonstrated after 20 months of frozen storage in broiler carcass rinsate alone. Therefore, naturally contaminated uncooked frozen poultry may pose a potential health hazard. C1 [Cox, N. A.; Richardson, L. J.; Berrang, M. E.; Rigsby, L. L.; Buhr, R. J.; Plumblee, J. R.; Fedorka-Cray, P. J.] ARS, Athens, GA 30605 USA. [Cox, N. A.; Richardson, L. J.; Berrang, M. E.; Rigsby, L. L.; Buhr, R. J.; Plumblee, J. R.; Fedorka-Cray, P. J.] USDA, Athens, GA USA. RP Cox, NA (reprint author), ARS, Athens, GA 30605 USA. EM nelson.cox@ars.usda.gov NR 15 TC 0 Z9 0 U1 2 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0149-6085 EI 1745-4565 J9 J FOOD SAFETY JI J. Food Saf. PD FEB PY 2014 VL 34 IS 1 BP 76 EP 78 DI 10.1111/jfs.12098 PG 3 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AB3XO UT WOS:000331723700011 ER PT J AU Kongoli, C Kustas, WP Anderson, MC Norman, JM Alfieri, JG Flerchinger, GN Marks, D AF Kongoli, Cezar Kustas, William P. Anderson, Martha C. Norman, John M. Alfieri, Joseph G. Flerchinger, Gerald N. Marks, Danny TI Evaluation of a Two-Source Snow-Vegetation Energy Balance Model for Estimating Surface Energy Fluxes in a Rangeland Ecosystem SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article DE Model evaluation/performance; Land surface model; Surface observations ID MAPPING DAILY EVAPOTRANSPIRATION; HEAT-FLUX; WATER-BALANCE; COVER; TEMPERATURE; SCALES; SOIL; EVAPORATION; CANOPY; SENSITIVITY AB The utility of a snow-vegetation energy balance model for estimating surface energy fluxes is evaluated with field measurements at two sites in a rangeland ecosystem in southwestern Idaho during the winter of 2007: one site dominated by aspen vegetation and the other by sagebrush. Model parameterizations are adopted from the two-source energy balance (TSEB) modeling scheme, which estimates fluxes from the vegetation and surface substrate separately using remotely sensed measurements of land surface temperature. Modifications include development of routines to account for surface snowmelt energy flux and snow masking of vegetation. Comparisons between modeled and measured surface energy fluxes of net radiation and turbulent heat showed reasonable agreement when considering measurement uncertainties in snow environments and the simplified algorithm used for the snow surface heat flux, particularly on a daily basis. There was generally better performance over the aspen field site, likely due to more reliable input data of snow depth/snow cover. The model was robust in capturing the evolution of surface energy fluxes during melt periods. The model behavior was also consistent with previous studies that indicate the occurrence of upward sensible heat fluxes during daytime owing to solar heating of vegetation limbs and branches, which often exceeds the downward sensible heat flux driving the snowmelt. However, model simulations over aspen trees showed that the upward sensible heat flux could be reversed for a lower canopy fraction owing to the dominance of downward sensible heat flux over snow. This indicates that reliable vegetation or snow cover fraction inputs to the model are needed for estimating fluxes over snow-covered landscapes. C1 [Kongoli, Cezar] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Kongoli, Cezar] NOAA, Natl Environm Satellite Data & Informat Serv, College Pk, MD 20740 USA. [Kustas, William P.; Anderson, Martha C.; Alfieri, Joseph G.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD USA. [Norman, John M.] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA. [Flerchinger, Gerald N.; Marks, Danny] ARS, Northwest Watershed Res Ctr, USDA, Boise, ID USA. RP Kongoli, C (reprint author), NOAA, NESDIS, NOAA Ctr Weather & Climate Predict NCWCP, 5830 Univ Res Ct 2804, College Pk, MD 20740 USA. EM cezar.kongoli@noaa.gov RI Anderson, Martha/C-1720-2015; Kustas, William/C-2063-2015 OI Anderson, Martha/0000-0003-0748-5525; FU USDA-ARS Hydrology and Remote Sensing Laboratory; NOAA Climate Program Office [60-1265-1-051]; NASA Applied Sciences Program [60-1245-3-053] FX This study would not have been possible without the field measurements provided by the USDA-ARS Northwest Watershed Research Center and financial support from the USDA-ARS Hydrology and Remote Sensing Laboratory, the NOAA Climate Program Office (Agreement 60-1265-1-051), and the NASA Applied Sciences Program (Agreement 60-1245-3-053). USDA is an equal opportunity provider and employer. NR 54 TC 1 Z9 1 U1 0 U2 12 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD FEB PY 2014 VL 15 IS 1 BP 143 EP 158 DI 10.1175/JHM-D-12-0153.1 PG 16 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA AC2BI UT WOS:000332301900008 ER PT J AU Teal, PEA Jones, D Jones, G Torto, B Nyasembe, V Borgemeister, C Alborn, HT Kaplan, F Boucias, D Lietze, VU AF Teal, Peter E. A. Jones, Davy Jones, Grace Torto, Baldwyn Nyasembe, Vincent Borgemeister, Christian Alborn, Hans T. Kaplan, Fatma Boucias, Drion Lietze, Verena U. TI Identification of Methyl Farnesoate from the Hemolymph of Insects SO JOURNAL OF NATURAL PRODUCTS LA English DT Article ID JUVENILE-HORMONE-III; BIOSYNTHESIS; BISEPOXIDE; SKELETON; INVITRO AB Methyl farnesoate, [methyl (2E,6E)-3,7,11-trimethyldode-ca-2,6,10-trienoate (1)] has not been I thought be present in the hemolymph of insects, although it is the immediate biosynthetic precursor of the circulating insect hormone juvenile hormone III (methyl (2E,6E)-10,11-epoxy-3,7,11-trimethyl-2,6-dodecadienoate) (2). Compound 1 was identified from the hemolymph obtained from five orders of insects. Identification of 1 from the American bird grasshopper was facilitated using both electron impact and chemical-ionization GC-MS, GC-FTIR, and 2D NMR techniques. The identifications from other insects were made using GC-MS, and the amounts of all were quantified using LIM-CI-GC-MS. The ratios of 1 and 2 varied in these insects during different developmental stages. The present results underscore the need for further studies on methyl farnesoate (1) as a circulating hormone in insects. C1 [Teal, Peter E. A.; Alborn, Hans T.; Kaplan, Fatma] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32604 USA. [Jones, Davy] Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40506 USA. [Jones, Grace] Univ Kentucky, Dept Biol, Lexington, KY 40506 USA. [Torto, Baldwyn; Nyasembe, Vincent; Borgemeister, Christian] Int Ctr Insect Physiol & Ecol, Nairobi, Kenya. [Boucias, Drion; Lietze, Verena U.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32608 USA. RP Teal, PEA (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1700 SW 23 Dr, Gainesville, FL 32604 USA. EM peter.teal@ars.usda.gov NR 17 TC 12 Z9 12 U1 1 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 EI 1520-6025 J9 J NAT PROD JI J. Nat. Prod. PD FEB PY 2014 VL 77 IS 2 BP 402 EP 405 DI 10.1021/np400807v PG 4 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA AC2SQ UT WOS:000332354700030 PM 24467367 ER PT J AU Mccann, BE Malek, MJ Newman, RA Schmit, BS Swafford, SR Sweitzer, RA Simmons, RB AF Mccann, Blake E. Malek, Mathew J. Newman, Robert A. Schmit, Brandon S. Swafford, Seth R. Sweitzer, Richard A. Simmons, Rebecca B. TI Mitochondrial Diversity Supports Multiple Origins for Invasive Pigs SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE diversity; feral; genetics; mitochondrial DNA; pig; Sus scrofa; United States ID COMPLETE MTDNA GENOME; WILD BOAR; SUS-SCROFA; PHYLOGENETIC-RELATIONSHIPS; DOMESTIC PIGS; DNA-SEQUENCE; FERAL PIGS; POPULATION-STRUCTURE; GENETIC-VARIATION; CONTROL REGION AB Our objective was to identify and evaluate mitochondrial diversity of wild pigs in the United States (U.S.). We obtained tissue samples from 81 individual pigs in 30 U.S. states and amplified a 403 base-pair region of mitochondrial DNA. We then downloaded overlapping sequences (n=904) from public repositories to create a global reference. We used parsimony and Bayesian techniques to evaluate phylogenetic relationships, and we used origins of published sequences from Eurasian wild boar to establish a phylogeographic reference. We then compared gene and nucleotide diversity measures for introduced pigs in North America with those of domestic swine, Eurasian wild boar, and feral pigs within broad-scale geographic groupings. We identified 15 haplotypes for introduced pigs, representing wild and domestic animals from >30 countries spanning the indigenous range of S. scrofa. Mitochondrial diversity measures and phylogenetic relationships indicated a strong association between introduced pigs and European domestic breeds, reflecting the known history of human colonization, trade, and settlement in the United States. Based on the geographic distribution of haplotypes in North America, we found that range expansion is a product of translocation from historical populations and introduction from new genetic sources. Finally, haplotype network analyses provided evidence of past demographic expansions within lineages, contributing to observed mtDNA variation among introduced pigs in North America. (c) 2014 The Wildlife Society. C1 [Mccann, Blake E.] Theodore Roosevelt Natl Pk, Wildlife, Resource Management, Medora, ND 58645 USA. [Malek, Mathew J.; Newman, Robert A.; Simmons, Rebecca B.] Univ N Dakota, Dept Biol, Grand Forks, ND 58202 USA. [Schmit, Brandon S.] Wildlife Serv, USDA, Ft Collins, CO 80521 USA. [Swafford, Seth R.] US Fish & Wildlife Serv, Yazoo City, MS 39194 USA. [Sweitzer, Richard A.] Great Basin Inst, Reno, NV 89511 USA. RP Mccann, BE (reprint author), Theodore Roosevelt Natl Pk, Wildlife, Resource Management, 315 Second Ave POB 7, Medora, ND 58645 USA. EM blake_mccann@nps.gov FU Dakota Experimental Program to Stimulate Competitive Research (ND EPSCoR); University of North Dakota Department of Biology; University of North Dakota Graduate School FX We thank North Dakota Experimental Program to Stimulate Competitive Research (ND EPSCoR), University of North Dakota Department of Biology, and University of North Dakota Graduate School for funding of this research. Thanks to United States Department of Agriculture, Wildlife Disease Program and field agents for collection of pig tissue samples from throughout the United States. Thanks also to the National Park Service, Institute for Wildlife Studies, North Dakota Game and Fish Department, and many other state and private organizations for collection of samples that made this work possible. We thank K. Drees, T. Jahraus, T. Mitchell, A. Bergstrom, and S. Johnson for laboratory and computational contributions. Finally, we thank I. Ovtchinnikov, R. Zink, V. Tkach, M. Cronin, and anonymous referees, for critical comments to improve this manuscript. NR 70 TC 4 Z9 4 U1 1 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2014 VL 78 IS 2 BP 202 EP 213 DI 10.1002/jwmg.651 PG 12 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AB5XS UT WOS:000331862600004 ER PT J AU Thomas, EH Brittingham, MC Stoleson, SH AF Thomas, Emily H. Brittingham, Margaret C. Stoleson, Scott H. TI Conventional Oil and Gas Development Alters Forest Songbird Communities SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Allegheny National Forest; biotic homogenization; community structure; forest fragmentation; forest songbirds; guilds; oil and gas development ID BIOTIC HOMOGENIZATION; BIRD COMMUNITIES; NORTHERN HARDWOODS; ENERGY DEVELOPMENT; UNITED-STATES; FRAGMENTATION; ABUNDANCE; CONSERVATION; LANDSCAPE; SELECTION AB Energy extraction within forest habitat is increasing at a rapid rate throughout eastern North America from the combined presence of conventional oil and gas, shale gas, and wind energy. We examined the effects of conventional oil and gas development on forest habitat including amounts of core and edge forest, the abundance of songbird species and guilds, species diversity, and community similarity within and between mixed hardwood and oak forest types at both individual wells (local scale) and at the 25-ha scale at differing levels of well density: reference (0wells/site, 0wells/km(2)), low (1-5wells/site, 4-20 wells/km(2)), and high (10-15wells/site, 40-60wells/km(2)). Amount of cleared area, length of roads, and amount of edge increased with increasing well density, whereas amount of core forest declined. At high well densities, 85% of the study site remained forested, but the mean amount of core forest declined from 68% to 2%. Specific changes to forest structure associated with oil and gas development included decreases in basal area and canopy cover within 20m of individual wells and with increasing well density. Of 19 species analyzed, 5 species, including ovenbird (Seiurus aurocapilla), Blackburnian warbler (Setophaga fusca), and black-throated green warbler (Setophaga virens), had lower abundance at well sites than reference sites at either the local or 25-ha scale. Six species including American robin (Turdus migratorius), chestnut-sided warbler (Setophaga pensylvanica), and brown-headed cowbird (Molothrus ater) were more abundant at well than reference sites. Eight species did not differ in abundance between well and reference sites. All 3 songbird guilds showed distinct patterns of abundance in relation to habitat differences resulting from oil and gas development that were consistent with known fragmentation effects. Forest interior species were less abundant at well sites than reference sites and showed a declining trend with increasing well density. In contrast, the guilds of early successional species and synanthropic species were more abundant at well sites than reference sites as was species richness (alpha diversity). Avian communities differed between northern hardwood and oak forest types at reference sites but became more similar when wells were present at both scales, suggesting biotic homogenization or a loss of beta diversity occurred as similar species were attracted to well sites in both forest types. The bird communities associated with northern hardwoods and oaks still retained their unique characteristics at low well densities but became similar at high well densities suggesting a threshold somewhere between the low and high well density sites. Consequently, we recommend that if well development is to occur in extensively forested landscapes, conventional oil and gas well development be limited to a maximum of 20wells/km(2) to minimize impacts to forest birds. (c) 2014 The Wildlife Society. C1 [Thomas, Emily H.; Brittingham, Margaret C.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Stoleson, Scott H.] US Forest Serv, Forestry Sci Lab, USDA, No Res Stn, Irvine, PA 16329 USA. RP Thomas, EH (reprint author), Penn State Univ, DuBois Campus,001 Multipurpose Bldg,1 Coll Pl, Du Bois, PA 15801 USA. EM mxb21@psu.edu FU U.S. Department of Agriculture Forest Service; Department of Ecosystem Science and Management; College of Agricultural Sciences at The Pennsylvania State University FX We are grateful to the U.S. Department of Agriculture Forest Service, and The Department of Ecosystem Science and Management and The College of Agricultural Sciences at The Pennsylvania State University for providing funding for this project. This project would not have been possible without the extensive efforts of S. Stout to secure funding to complete this research. We thank our field technicians K. Meley, T. Schreckengost, N. Fronk, and M. Yakich and coworkers L. Ordiway and D. Watts for their help with point counts and vegetation measurements. We thank W. Tzilkowski, M. Marshall, S. Pabian, A. Weber, and K. Yoder for their valuable input throughout this research. Thoughtful reviews by P. Porneluzi and T. O'Connell greatly improved and focused this manuscript. Finally, we are grateful to everyone at the Forestry Sciences Lab in Irvine, Pennsylvania for their continued support and enthusiasm for our research. NR 71 TC 7 Z9 7 U1 9 U2 74 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2014 VL 78 IS 2 BP 293 EP 306 DI 10.1002/jwmg.662 PG 14 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AB5XS UT WOS:000331862600013 ER PT J AU Smetzer, JR King, DI Schlossberg, S AF Smetzer, Jennifer R. King, David I. Schlossberg, Scott TI Management Regime Influences Shrubland Birds and Habitat Conditions in the Northern Appalachians, USA SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE clearcut; early-successional habitat; management history; N-mixture models; silviculture; wildlife opening ID NORTHEASTERN UNITED-STATES; EARLY-SUCCESSIONAL HABITATS; REPLICATED COUNTS; SONGBIRD RESPONSE; HARDWOOD FOREST; MIXTURE-MODELS; NEW-HAMPSHIRE; OF-WAYS; COMMUNITIES; MASSACHUSETTS AB Population declines of birds that breed in early-successional shrubland habitat are of great concern to conservationists throughout the northeastern United States. To help increase the efficiency and effectiveness of efforts to conserve these species and their habitats, we studied birds in temporary forest openings created through even-aged timber harvest, and permanent wildlife openings maintained through mechanical treatment and prescribed burning in the Northern Appalachians, USA in 2010 and 2011. We assessed the effects of treatment method, time since last treatment, and retained tree cover on shrubland bird abundance and habitat conditions. Burned and mechanically treated wildlife openings differed only in grass and fern cover. Both types of wildlife openings had more grasses and forbs, and less bare ground than silvicultural openings. Six out of 8 focal bird species were less abundant in silvicultural openings than in wildlife openings. In contrast, abundance of only 1 species differed between burned and mechanically treated wildlife openings. Silvicultural openings supported the same species as wildlife openings, indicating that this management option could be used in place of more costly wildlife opening management. However, because birds were more abundant in wildlife openings, maintaining the current population size of shrubland birds under a management strategy based entirely on silviculture would require a 50-300% increase in the area of silvicultural openings, depending on the species. Individual species peaked in abundance at different times post treatment, indicating that managers must maintain a range of early-successional conditions across the landscape to provide habitat for the entire suite of shrubland birds. Six species exhibited a negative relationship with the basal area of retained conifer cover, and 7 species with deciduous tree cover, indicating that the retention of overstory trees in openings reduced their utility to early-successional birds. (c) 2014 The Wildlife Society, 2014. C1 [Smetzer, Jennifer R.; Schlossberg, Scott] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [King, David I.] Univ Massachusetts, US Forest Serv Northern Res Stn, Amherst, MA 01003 USA. RP Smetzer, JR (reprint author), Univ Massachusetts, Dept Environm Conservat, 204 Holdsworth Hall, Amherst, MA 01003 USA. EM jsmetzer@eco.umass.edu FU USDA Northern Research Station; Green Mountain National Forest FX L. Nolan, A. Richardson, R. Hepner, K. Forster, and A. Yorks provided excellent help with data collection, and J. Sease provided logistical support in the field. B. Mazzei and B. Thompson provided helpful suggestions for improving the manuscript. Funding for the work was provided by the USDA Northern Research Station and the Green Mountain National Forest. NR 53 TC 2 Z9 2 U1 4 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2014 VL 78 IS 2 BP 314 EP 324 DI 10.1002/jwmg.658 PG 11 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AB5XS UT WOS:000331862600015 ER PT J AU Mckelvey, KS Aubry, KB Anderson, NJ Clevenger, AP Copeland, JP Heinemeyer, KS Inman, RM Squires, JR Waller, JS Pilgrim, KL Schwartz, MK AF Mckelvey, Kevin S. Aubry, Keith B. Anderson, Neil J. Clevenger, Anthony P. Copeland, Jeffrey P. Heinemeyer, Kimberley S. Inman, Robert M. Squires, John R. Waller, John S. Pilgrim, Kristine L. Schwartz, Michael K. TI Recovery of Wolverines in the Western United States: Recent Extirpation and Recolonization or Range Retraction and Expansion? SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE extirpated; Gulo; haplotype; historical; introduction; mitochondrial; wolverine ID NORTHWESTERN NORTH-AMERICA; GULO-GULO POPULATIONS; GREAT-BASIN; ANCIENT DNA; GENETIC-VARIABILITY; SIERRA-NEVADA; PHYLOGEOGRAPHY; CALIFORNIA; PLEISTOCENE; MAMMALS AB Wolverines were greatly reduced in number and possibly extirpated from the contiguous United States (U.S.) by the early 1900s. Wolverines currently occupy much of their historical range in Washington, Idaho, Montana, and Wyoming, but are absent from Utah and only single individuals are known to occur in California and Colorado. In response, the translocation of wolverines to California and Colorado is being considered. If wolverines are to be reintroduced, managers must identify appropriate source populations based on the genetic affinities of historical and modern wolverine populations. We amplified the mitochondrial control region of 13 museum specimens dating from the late 1800s to early 1900s and 209 wolverines from modern populations in the contiguous U.S. and Canada and combined results with previously published haplotypes. Collectively, these data indicated that historical wolverine populations in the contiguous U.S. were extirpated by the early 20th century, and that modern populations in the contiguous U.S. are likely the descendants of recent immigrants from the north. The Cali1 haplotype previously identified in California museum specimens was also common in historical samples from the southern Rocky Mountains, and likely evolved in isolation in the southern ice-free refugium that encompassed most of the contiguous U.S. during the last glaciation. However, when southern populations were extirpated, these matrilines were eliminated. Several of the other haplotypes found in historical specimens from the contiguous U.S. also occur in modern North American populations, and belong to a group of haplotypes that are associated with the rapid expansion of northern wolverine populations after the last glacial retreat. Modern wolverines in the contiguous U.S. are primarily haplotype A, which is the most common and widespread haplotype in Canada and Alaska. For the translocation of wolverines to California, Colorado, and other areas in the western U.S., potential source populations in the Canadian Rocky Mountains may provide the best mix of genetic diversity and appropriate learned behavior. (c) 2014 The Wildlife Society. C1 [Mckelvey, Kevin S.; Squires, John R.; Pilgrim, Kristine L.; Schwartz, Michael K.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Aubry, Keith B.] US Forest Serv, Pacific NW Res Stn, Olympia, WA 98512 USA. [Anderson, Neil J.] Montana Fish Wildlife & Parks, Bozeman, MT 59717 USA. [Clevenger, Anthony P.] Montana State Univ, Western Transportat Inst, Bozeman, MT 59715 USA. [Copeland, Jeffrey P.] Wolverine Fdn, Tetonia, ID 83452 USA. [Heinemeyer, Kimberley S.] Round River Conservat Studies, Salt Lake City, UT 84103 USA. [Inman, Robert M.] Swedish Univ Agr Sci, Wildlife Conservat Soc, Ennis, MT 59729 USA. [Inman, Robert M.] Swedish Univ Agr Sci, Grimso Wildlife Res Stn, Dept Ecol, Ennis, MT 59729 USA. [Waller, John S.] Natl Pk Serv, West Glacier, MT 59936 USA. RP Mckelvey, KS (reprint author), US Forest Serv, Rocky Mt Res Stn, 800 East Beckwith Ave, Missoula, MT 59801 USA. EM kmckelvey@fs.fed.us RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 NR 62 TC 2 Z9 2 U1 10 U2 82 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2014 VL 78 IS 2 BP 325 EP 334 DI 10.1002/jwmg.649 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AB5XS UT WOS:000331862600016 ER PT J AU Ruiz-May, E Hucko, S Howe, KJ Zhang, S Sherwood, RW Thannhauser, TW Rose, JKC AF Ruiz-May, Eliel Hucko, Simon Howe, Kevin J. Zhang, Sheng Sherwood, Robert W. Thannhauser, Theodore W. Rose, Jocelyn K. C. TI A Comparative Study of Lectin Affinity Based Plant N-Glycoproteome Profiling Using Tomato Fruit as a Model SO MOLECULAR & CELLULAR PROTEOMICS LA English DT Article ID CELL-WALL; ARABIDOPSIS-THALIANA; SECRETORY PATHWAY; MASS-SPECTROMETRY; PROTEIN GLYCOSYLATION; MEMBRANE-PROTEINS; REVEALS; CHLOROPLAST; EXPRESSION; PROTEOMICS AB Lectin affinity chromatography (LAC) can provide a valuable front-end enrichment strategy for the study of N-glycoproteins and has been used to characterize a broad range eukaryotic N-glycoproteomes. Moreover, studies with mammalian systems have suggested that the use of multiple lectins with different affinities can be particularly effective. A multi-lectin approach has also been reported to provide a significant benefit for the analysis of plant N-glycoproteins; however, it has yet to be determined whether certain lectins, or combinations of lectins are optimal for plant N-glycoproteome profiling; or whether specific lectins show preferential association with particular N-glycosylation sites or N-glycan structures. We describe here a comparative study of three mannose-binding lectins, concanavalin A, snowdrop lectin, and lentil lectin, to profile the N-glycoproteome of mature green stage tomato (Solanum lycopersicum) fruit pericarp. Through coupling lectin affinity chromatography with a shotgun proteomics strategy, we identified 448 putative N-glycoproteins, whereas a parallel lectin affinity chromatography plus hydrophilic interaction chromatography analysis revealed 318 putative N-glycosylation sites on 230 N-glycoproteins, of which 100 overlapped with the shotgun analysis, as well as 17 N-glycan structures. The use of multiple lectins substantially increased N-glycoproteome coverage and although there were no discernible differences in the structures of N-glycans, or the charge, isoelectric point (pI) or hydrophobicity of the glycopeptides that differentially bound to each lectin, differences were observed in the amino acid frequency at the -1 and +1 subsites of the N-glycosylation sites. We also demonstrated an alternative and complementary in planta recombinant expression strategy, followed by affinity MS analysis, to identify the putative N-glycan structures of glycoproteins whose abundance is too low to be readily determined by a shotgun approach, and/or combined with deglycosylation for predicted deamidated sites, using a xyloglucan-specific endoglucanase inhibitor protein as an example. C1 [Ruiz-May, Eliel; Rose, Jocelyn K. C.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Hucko, Simon; Howe, Kevin J.; Thannhauser, Theodore W.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Zhang, Sheng; Sherwood, Robert W.] Inst Biotechnol, Prote & Mass Spectrometry Facil, Ithaca, NY 14853 USA. RP Rose, JKC (reprint author), Cornell Univ, Dept Plant Biol, 412 Mann Lib Bldg, Ithaca, NY 14853 USA. EM jr286@cornell.edu FU National Science Foundation Plant Genome Program [DBI-0606595]; USDA [1907-21000-37-00D]; New York State Office of Science, Technology and Academic Research (NYSTAR) FX This work has been supported by grants from the National Science Foundation Plant Genome Program (DBI-0606595), the USDA (1907-21000-37-00D), and the New York State Office of Science, Technology and Academic Research (NYSTAR). NR 74 TC 12 Z9 13 U1 1 U2 26 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 1535-9476 EI 1535-9484 J9 MOL CELL PROTEOMICS JI Mol. Cell. Proteomics PD FEB PY 2014 VL 13 IS 2 BP 566 EP 579 DI 10.1074/mcp.M113.028969 PG 14 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA AA8TX UT WOS:000331369000014 PM 24198434 ER PT J AU Zhang, LS Li, XW Zhang, LX Li, BZ Han, MY Liu, FT Zheng, P Alva, AK AF Zhang, Linsen Li, Xuewei Zhang, Lixin Li, Bingzhi Han, Mingyu Liu, Futing Zheng, Peng Alva, Ashok K. TI ROLE OF ABSCISIC ACID (ABA) IN MODULATING THE RESPONSES OF TWO APPLE ROOTSTOCKS TO DROUGHT STRESS SO PAKISTAN JOURNAL OF BOTANY LA English DT Article ID ANTIOXIDANT SYSTEM; LIPID-PEROXIDATION; MALUS-DOMESTICA; SIGNAL-TRANSDUCTION; HYDROGEN-PEROXIDE; STOMATAL CLOSURE; WATER-DEFICIT; NITRIC-OXIDE; L.; LEAVES AB Drought stress is considered as the main limiting factor for apple (Malus domestica L.) production in some semi-arid areas of China. In this study, we investigated the modulation role of abscisic acid (ABA) and fluridone (ABA synthesis inhibitor) on water relations and antioxidant enzyme system in 2-year-old seedlings of two apple rootstocks i.e. Malus sieversii (Ledeb.) Roem. (MS) and Malus hupehensis (Pamp.) Rehd. (MH). Drought stress induced ion leakage, accumulation of malondiadehyde (MDA) and decreases in leaf water potential and relative water content (RWC) in both rootstocks, which were significantly alleviated by exogenous ABA application. Drought stress also induced markedly increases in endogenous ABA content and activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), and glutathione reductase (GR), to a greater magnitude in MS as compared to MH rootstock. Concentration of 100 mu mol/L and 50 mu mol/L ABA had the most positive effects on drought-stressed rootstocks of MS and MH, respectively. Spraying optimum exogenous ABA contributed to enhancement in most of the above antioxidant enzymes activities but reduction in content of MDA and maintained the appropriate leaf water potential and RWC in both rootstocks. Pretreatment with fluridone aggravated ion leakage and the accumulation of MDA in two apple rootstocks under drought stress, which was overcome by exogenous ABA application to some extent. In conclusion, the endogenous ABA was probably involved in the regulation of two apple rootstocks in responses to drought stress. C1 [Zhang, Linsen; Li, Xuewei; Li, Bingzhi; Han, Mingyu; Liu, Futing] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China. [Zhang, Lixin; Zheng, Peng] Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China. [Alva, Ashok K.] USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. RP Zhang, LX (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China. EM zhanglixin@nwsuaf.edu.cn FU Special Fund for Agro-scientific Research in the Public Interest [201303104]; National Apple Industry Technology System [CARS-28]; Sci-tech Coordinating Innovative Engineering Projects of Shaanxi Province [2011KTZB02-02-05]; Program for Agricultural Sci-Tech Innovation of Shaanxi Province [2011NXC01-18]; West Light Foundation of CAS [2060299-14]; Environment Protection Program [2012-47] FX This research was supported by the Special Fund for Agro-scientific Research in the Public Interest (201303104), National Apple Industry Technology System (CARS-28), Sci-tech Coordinating Innovative Engineering Projects of Shaanxi Province (2011KTZB02-02-05), Program for Agricultural Sci-Tech Innovation of Shaanxi Province (2011NXC01-18), West Light Foundation of CAS (2060299-14) and Environment Protection Program (2012-47). LINSEN ZHANG and XUEWEI LI contributed to this work equally. NR 51 TC 1 Z9 1 U1 1 U2 14 PU PAKISTAN BOTANICAL SOC PI KARACHI PA DEPT OF BOTANY UNIV KARACHI, 32 KARACHI, PAKISTAN SN 0556-3321 EI 2070-3368 J9 PAK J BOT JI Pak. J. Bot. PD FEB PY 2014 VL 46 IS 1 BP 117 EP 126 PG 10 WC Plant Sciences SC Plant Sciences GA AB8VG UT WOS:000332067500012 ER PT J AU Jiang, T Wei, SQ Flanagan, DC Li, MJ Li, XM Wang, Q Luo, C AF Jiang Tao Wei Shi-Qiang Flanagan, D. C. Li Meng-Jie Li Xue-Mei Wang Qiang Luo Chang TI Effect of Abiotic Factors on the Mercury Reduction Process by Humic Acids in Aqueous Systems SO PEDOSPHERE LA English DT Article DE abiotic reduction; complexation; humic substance; natural organic matter ID DISSOLVED ORGANIC-MATTER; SUBSTANCES RIO-NEGRO; GASEOUS MERCURY; ELECTRON-ACCEPTORS; DIVALENT MERCURY; REDOX PROPERTIES; FULVIC-ACIDS; FRESH-WATER; SOIL; IRON AB As a global pollutant process, the reduction of mercury (Hg) is especially important. One pathway is through an abiotic reduction with humic acids (HAs), which is controlled by different factors, including initial Hg and HA concentrations, pH, temperature and light. In this study, three humic acids were selected to illustrate the Hg2+ abiotic reduction mechanisms by HAs, and to identify the key limiting factors for reduction rates and amounts. In addition, the initial status of the HAs as a solid or in an aqueous solution were also compared, to help explain why HAs show different dominant characteristics (e.g. complexation or reduction) in the reaction process with Hg. Results indicated that HAs were able to reduce Hg abiotically. Higher initial Hg, higher HA concentrations and either high (8.1) or low (3.6) solution pH decreased the HA reduction capacity. In addition, Hg-0 production rates increased with increasing temperature, and the same trend was observed with light exposure. Humic acids added as an aqueous solution resulted in significantly greater Hg-0 production than addition as a bulk solid. Finally, the Hg reduction rate and capacity varied significantly (P < 0.05) with HAs from different sources. These findings helped to explain why HAs showed different dominant characteristics (e.g. complexation or reduction) in the reaction process with Hg, and evidentially demonstrated the existence of a possible pathway of Hg2+ reduction, which indicated that humic substances in natural environments, especially in water bodies, could act either as a sink or a source for Hg. C1 [Jiang Tao; Wei Shi-Qiang; Li Meng-Jie; Li Xue-Mei; Wang Qiang; Luo Chang] Southwest Univ, Dept Environm Sci & Engn, Coll Resources & Environm, Chongqing 400716, Peoples R China. [Jiang Tao; Wei Shi-Qiang; Wang Qiang] Chongqing Key Lab Agr Resources & Environm, Chongqing 400716, Peoples R China. [Flanagan, D. C.] USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Wei, SQ (reprint author), Southwest Univ, Dept Environm Sci & Engn, Coll Resources & Environm, Chongqing 400716, Peoples R China. EM sqwei@swu.edu.cn FU National Natural Science Foundation of China [40971147/D010503]; China Postdoctoral Science Foundation [2013M542238]; Fundamental Research Funds for The Central Universities, China [XDJK2013C151]; Ph.D. Initiation Fund of Southwest University, China [SWU112098] FX Supported by the National Natural Science Foundation of China (No. 40971147/D010503), the China Postdoctoral Science Foundation (No. 2013M542238), the Fundamental Research Funds for The Central Universities, China (No. XDJK2013C151) and the Ph.D. Initiation Fund of Southwest University, China (No. SWU112098). NR 76 TC 4 Z9 5 U1 1 U2 29 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1002-0160 EI 2210-5107 J9 PEDOSPHERE JI Pedosphere PD FEB PY 2014 VL 24 IS 1 BP 125 EP 136 PG 12 WC Soil Science SC Agriculture GA AC3QX UT WOS:000332437400012 ER PT J AU Cernadas, RA Doyle, EL Nino-Liu, DO Wilkins, KE Bancroft, T Wang, L Schmidt, CL Caldo, R Yang, B White, FF Nettleton, D Wise, RP Bogdanove, AJ AF Cernadas, Raul A. Doyle, Erin L. Nino-Liu, David O. Wilkins, Katherine E. Bancroft, Timothy Wang, Li Schmidt, Clarice L. Caldo, Rico Yang, Bing White, Frank F. Nettleton, Dan Wise, Roger P. Bogdanove, Adam J. TI Code-Assisted Discovery of TAL Effector Targets in Bacterial Leaf Streak of Rice Reveals Contrast with Bacterial Blight and a Novel Susceptibility Gene SO PLOS PATHOGENS LA English DT Article ID ORYZAE PV.-ORYZAE; XANTHOMONAS-ORYZAE; RESISTANCE GENE; PATHOGEN INTERACTIONS; SULFATE TRANSPORTER; CELL-DEATH; HOST; DNA; EXPRESSION; RECOGNITION AB Bacterial leaf streak of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is an increasingly important yield constraint in this staple crop. A mesophyll colonizer, Xoc differs from X. oryzae pv. oryzae (Xoo), which invades xylem to cause bacterial blight of rice. Both produce multiple distinct TAL effectors, type III-delivered proteins that transactivate effector-specific host genes. A TAL effector finds its target(s) via a partially degenerate code whereby the modular effector amino acid sequence identifies nucleotide sequences to which the protein binds. Virulence contributions of some Xoo TAL effectors have been shown, and their relevant targets, susceptibility (S) genes, identified, but the role of TAL effectors in leaf streak is uncharacterized. We used host transcript profiling to compare leaf streak to blight and to probe functions of Xoc TAL effectors. We found that Xoc and Xoo induce almost completely different host transcriptional changes. Roughly one in three genes upregulated by the pathogens is preceded by a candidate TAL effector binding element. Experimental analysis of the 44 such genes predicted to be Xoc TAL effector targets verified nearly half, and identified most others as false predictions. None of the Xoc targets is a known bacterial blight S gene. Mutational analysis revealed that Tal2g, which activates two genes, contributes to lesion expansion and bacterial exudation. Use of designer TAL effectors discriminated a sulfate transporter gene as the S gene. Across all targets, basal expression tended to be higher than genome-average, and induction moderate. Finally, machine learning applied to real vs. falsely predicted targets yielded a classifier that recalled 92% of the real targets with 88% precision, providing a tool for better target prediction in the future. Our study expands the number of known TAL effector targets, identifies a new class of S gene, and improves our ability to predict functional targeting. Author Summary Many crop and ornamental plants suffer losses due to bacterial pathogens in the genus Xanthomonas. Pathogen manipulation of host gene expression by injected proteins called TAL effectors is important in many of these diseases. A TAL effector finds its gene target(s) by virtue of structural repeats in the protein that differ one from another at two amino acids that together identify one DNA base. The number of repeats and those amino acids thereby code for the DNA sequence the protein binds. This code allows target prediction and engineering TAL effectors for custom gene activation. By combining genome-wide analysis of gene expression with TAL effector binding site prediction and verification using designer TAL effectors, we identified 19 targets of TAL effectors in bacterial leaf streak of rice, a disease of growing importance worldwide caused by X. oryzae pv. oryzicola. Among these was a sulfate transport gene that plays a major role. Comparison of true vs. false predictions using machine learning yielded a classifier that will streamline TAL effector target identification in the future. Probing the diversity and functions of such plant genes is critical to expand our knowledge of disease and defense mechanisms, and open new avenues for effective disease control. C1 [Cernadas, Raul A.; Doyle, Erin L.; Nino-Liu, David O.; Wang, Li; Schmidt, Clarice L.; Caldo, Rico; Wise, Roger P.; Bogdanove, Adam J.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Cernadas, Raul A.; Wilkins, Katherine E.; Wang, Li; Bogdanove, Adam J.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. [Doyle, Erin L.] Iowa State Univ, Bioinformat & Computat Biol Grad Program, Ames, IA USA. [Bancroft, Timothy; Nettleton, Dan] Iowa State Univ, Dept Stat, Ames, IA USA. [Yang, Bing] Iowa State Univ, Ames, IA USA. [White, Frank F.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Wise, Roger P.] Iowa State Univ, USDA ARS, Ames, IA USA. RP Cernadas, RA (reprint author), Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. EM ajb7@cornell.edu FU National Science Foundation Plant Genome Research Program awards [0227357, 0820831, 0500461]; USDA-ARS CRIS [3625-21000-035-00D] FX This work was supported by National Science Foundation Plant Genome Research Program awards 0227357 (AJB), 0820831 (FFW, AJB, BY, DN), and 0500461 (RPW, DN), and USDA-ARS CRIS project 3625-21000-035-00D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 25 Z9 26 U1 4 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD FEB PY 2014 VL 10 IS 2 AR e1003972 DI 10.1371/journal.ppat.1003972 PG 24 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA AB9CC UT WOS:000332085900041 PM 24586171 ER PT J AU Tyree, MC Seiler, JR Maier, CA AF Tyree, Michael C. Seiler, John R. Maier, Chris A. TI Contrasting genotypes, soil amendments, and their interactive effects on short-term total soil CO2 efflux in a 3-year-old Pinus taeda L. plantation SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Clones; Fertilization; Loblolly pine; Logging residue; Microbial activity; Soil respiration ID BELOW-GROUND CARBON; LOBLOLLY-PINE; NUTRIENT AVAILABILITY; MICROBIAL BIOMASS; ORGANIC-MATTER; FINE-ROOT; RESIDUE MANAGEMENT; VIRGINIA PIEDMONT; SITE PREPARATION; LEAF PHYSIOLOGY AB Intensively managed pine forests in the southeastern United States are considered an important C sink and may play a critical role in offsetting increased global CO2 emissions. The combination of improved silvicultural methods and the use of superior genotypes are estimated to result in future volume gains of up to 60 percent. However to date, no work has looked at whether selection of elite genotypes could influence soil C dynamics, which could decrease the time necessary for the stand to function as a C sink. We evaluated the effects of contrasting loblolly pine genotypes on total soil surface CO2 efflux (F-S) and heterotrophic respiration (R-H) under two soil amendment treatments: 1.) fertilization and 2.) logging residue (LR) incorporation. We found an immediate and sustained difference in F-S (p = 0.05) and R-H (p < 0.01) among our two genotypes throughout the first two years of stand development. Our soil amendment treatments did not significantly change F-S, but did influence R-H. LR increased (p = 0.05) R-H while N and P fertilization induced a slight (p = 0.06) decrease throughout the study. Our genotypes differed (p = 0.05) in their temperature response of F-S, which resulted in an 11% difference in total cumulative C loss from the soil over the duration of the study. We hypothesize that observed treatment effects in F-S and R-H are largely due to differences in belowground C allocation among genotypes, which is supported by others that have looked at fine-root standing crop and turnover on these same genotypes. This work underscores the importance of accounting for differences among genotypes when developing stand-level C estimates. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Tyree, Michael C.; Seiler, John R.] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Maier, Chris A.] USDA Forest Serv, Southern Res Stn, Res Triangle Pk, NC 27709 USA. RP Tyree, MC (reprint author), Louisiana Tech Univ, Sch Forestry, POB 10138, Ruston, LA 71272 USA. EM mtyree@latech.edu; jseiler@vt.edu; cmaier@fs.fed.us FU USDA Forest Service FX The authors thank the USDA Forest Service, Agenda 2020 for funding this research and MeadWestvaco for their monumental effort in preparing, maintaining, and providing access to the study site. Specifically, thanks to Steve Patterson, Phil Dougherty, Kurt Johnsen, Daniel McInnis, and Karen Sarsony for their technical support. Special thanks to John Peterson, Ben Templeton, Jeremy Stovall, and David Jones for their help with field work, and Sarah Seiler, Stephanie Worthington, and Kevin Reidel for their assistance in the lab. Additional, thanks to the two anonymous reviewers for their valuable suggestions and comments. NR 47 TC 3 Z9 3 U1 1 U2 18 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD FEB PY 2014 VL 69 BP 93 EP 100 DI 10.1016/j.soilbio.2013.10.050 PG 8 WC Soil Science SC Agriculture GA AC1MV UT WOS:000332261600012 ER PT J AU Reardon, CL Gollany, HT Wuest, SB AF Reardon, C. L. Gollany, H. T. Wuest, S. B. TI Diazotroph community structure and abundance in wheat-fallow and wheat-pea crop rotations SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Wheat; Diazotroph; nifH; Nitrogen-fixation; Pea; Pacific Northwest ID BIOLOGICAL NITROGEN-FIXATION; NON-SYMBIOTIC N-2-FIXATION; N-2-FIXING BACTERIA; FERTILIZER INPUTS; FIXING BACTERIA; FOREST SOILS; DIVERSITY; CARBON; DENITRIFICATION; AVAILABILITY AB Biological input of nitrogen (N) from the atmosphere by free-living diazotrophs can help alleviate fertilizer use in agricultural systems. In this study, we investigated the effect of N fertilizer and winter pea (Pisum sativum L.) crop on the community structure and abundance of free-living diazotrophs in a two year study of diyland winter wheat (Triticum aestivum L.) no-till production system in Eastern Oregon, USA. Based on quantification of the nifH gene, diazotroph abundance was strongly influenced by plant species and the crop year in which the soil samples were collected. A greater amount of nifH copies was recovered in 2012 compared to 2011 either as copies per gram soil or normalized to the abundance of bacterial 16S rRNA genes. The quantity of genes was greater under pea than wheat in 2012 although no difference was observed in the preceding year. The nifH gene abundance was positively correlated to ammonium concentration in 2011 and bacterial abundance in 2012. Nitrogen application did not influence diazotroph abundance in the top 0-5 cm; however the abundance was reduced by application at the lower 5-10 cm depth under wheat crop. The diazotroph community structure appeared to be influenced more by N fertilization rather than plant species with the exception of wheat in 2012. Changes in the community structure over the two years were greater for fertilized than unfertilized soil. Collectively, these data suggest that year-to-year variability had a greater influence on diazotroph communities rather than specific parameters of plant species, fertilization, total N, total organic C, or soil pH. Multi-year studies are necessary to define the specific drivers of diazotroph abundance, community structure and function. Published by Elsevier Ltd. C1 [Reardon, C. L.; Gollany, H. T.; Wuest, S. B.] USDA ARS, Columbia Plateau Conservat Res Ctr, Adams, OR 97810 USA. RP Reardon, CL (reprint author), USDA ARS, POB 370, Pendleton, OR 97801 USA. EM catherine.reardon@ars.usda.gov NR 43 TC 15 Z9 17 U1 8 U2 64 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD FEB PY 2014 VL 69 BP 406 EP 412 DI 10.1016/j.soilbio.2013.10.038 PG 7 WC Soil Science SC Agriculture GA AC1MV UT WOS:000332261600046 ER PT J AU Renninger, HJ Carlo, N Clark, KL Schafer, KVR AF Renninger, Heidi J. Carlo, Nicholas Clark, Kenneth L. Schaefer, Karina V. R. TI Physiological strategies of co-occurring oaks in a water- and nutrient-limited ecosystem SO TREE PHYSIOLOGY LA English DT Article DE nitrogen-use efficiency; photosynthesis; Quercus; sap flow; water-use efficiency ID CARBON-ISOTOPE DISCRIMINATION; NITROGEN-USE-EFFICIENCY; DIFFUSE-POROUS TREES; NEW-JERSEY PINELANDS; TROPICAL DRY FOREST; QUERCUS-ILEX; SAP-FLOW; STOMATAL CONDUCTANCE; PHOTOSYNTHETIC PARAMETERS; CANOPY TRANSPIRATION AB Oak species are well suited to water-limited conditions by either avoiding water stress through deep rooting or tolerating water stress through tight stomatal control. In co-occurring species where resources are limited, species may either partition resources in space and/or time or exhibit differing efficiencies in the use of limited resources. Therefore, this study seeks to determine whether two co-occurring oak species (Quercus prinus L. and Quercus velutina Lam.) differ in physiological parameters including photosynthesis, stomatal conductance, water-use (WUE) and nitrogen-use efficiency (NUE), as well as to characterize transpiration and average canopy stomatal responses to climatic variables in a sandy, well-drained and nutrient-limited ecosystem. The study was conducted in the New Jersey Pinelands and we measured sap flux over a 3-year period, as well as leaf gas exchange, leaf nitrogen and carbon isotope concentrations. Both oak species showed relatively steep increases in leaf-specific transpiration at low vapor pressure deficit (VPD) values before maximum transpiration rates were achieved, which were sustained over a broad range in VPD. This suggests tight stomatal control over transpiration in both species, although Q. velutina showed significantly higher leaf-level and canopy-level stomatal conductance than Q. prinus. Average daytime stomatal conductance was positively correlated with soil moisture and both oak species maintained at least 75% of their maximum canopy stomatal conductance at soil moistures in the upper soil layer (0-0.3 m) as low as 0.03 m(3) m(3-3). Quercus velutina had significantly higher photosynthetic rates, maximum Rubisco-limited and electron-transport-limited carboxylation rates, dark respiration rates and nitrogen concentration per unit leaf area than Q. prinus. However, both species exhibited similar WUEs and NUEs. Therefore, Q. prinus has a more conservative resource-use strategy, while Q. velutina may need to exploit niches that are locally higher in nutrients and water. Likewise, both species appear to tap deep, stable water sources, highlighting the importance of rooting depth in modeling transpiration and stomatal conductance in many oak ecosystems. C1 [Renninger, Heidi J.; Schaefer, Karina V. R.] Rutgers State Univ, Dept Biol Sci, Newark, NJ 07102 USA. [Carlo, Nicholas; Schaefer, Karina V. R.] Rutgers State Univ, Dept Earth & Environm Sci, Newark, NJ 07102 USA. [Clark, Kenneth L.] US Forest Serv, USDA, No Res Stn, New Lisbon, NJ 08064 USA. RP Renninger, HJ (reprint author), Rutgers State Univ, Dept Biol Sci, 195 Univ Ave, Newark, NJ 07102 USA. EM hrenninger@gmail.com RI Renninger, Heidi/E-5237-2015 OI Renninger, Heidi/0000-0002-2485-9835 FU USDA Forest Service [10-JV-11242306-136]; Office of Science (BER), US Department of Energy [DE-SC0007041] FX This work was funded by the following grants: USDA Forest Service joint venture agreement 10-JV-11242306-136 and Office of Science (BER), US Department of Energy DE-SC0007041 to K.V.R.S. NR 67 TC 11 Z9 11 U1 4 U2 49 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD FEB PY 2014 VL 34 IS 2 BP 159 EP 173 DI 10.1093/treephys/tpt122 PG 15 WC Forestry SC Forestry GA AC3KN UT WOS:000332417200006 PM 24488856 ER PT J AU Guimaraes, IG Lim, C Yildirim-Aksoy, M Li, MH Klesius, PH AF Guimaraes, I. G. Lim, C. Yildirim-Aksoy, M. Li, M. H. Klesius, P. H. TI Effects of dietary levels of vitamin A on growth, hematology, immune response and resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae SO ANIMAL FEED SCIENCE AND TECHNOLOGY LA English DT Article DE Nile tilapia; Vitamin A; Growth performance; Hematology; Immune response; Disease resistance ID SALMO-SALAR L; ATLANTIC SALMON; RAINBOW-TROUT; BETA-CAROTENE; SERUM; IMMUNOCOMPETENCE; ASTAXANTHIN; DEFICIENCY; LYSOZYME AB This study was conducted to evaluate the effect of supplemental levels of vitamin A (0,2500, 5000, 10,000, and 20,000 IU/kg diet) on the growth performance, hematology, immune response and resistance of Nile tilapia, Oreochromis niloticus to Streptococcus iniae challenge. Each diet was fed to Nile tilapia (initial weight, 5.26 +/- 0.10 g) in quadruplicate aquaria to apparent satiation twice daily for 10 weeks. The results indicate that vitamin A is a dietary essential for Nile tilapia. After 2 weeks of feeding, fish fed the vitamin A unsupplemented diet exhibited significantly lowest weight gain, feed intake and feed efficiency ratio than those of other treatments. Dietary vitamin A levels had no effect on survival. Other gross deficiency signs observed during the 10-week trial were hemorrhages, operculum deformity, fin erosion, darker body coloration and lethargy. Significantly lower hepatosomatic index and hematological parameters (except white blood cell count) were also recorded in fish fed the vitamin A unsupplemented diet. The resistance of erythrocytes to hemolysis in hypotonic solutions was lowest in fish fed vitamin A unsupplemented diet and increased with increasing vitamin A supplementation. A supplemental level of vitamin A of 3910 IU/kg diet was sufficient to prevent these deficiency signs in juvenile Nile tilapia. Serum protein, lysozyme activity and superoxide anion production were enhanced by supplementation of vitamin A, whilst serum total immunoglobulin and spontaneous complement activity were not affected by dietary treatments. Dietary supplementation of vitamin A had no effect on the resistance of Nile tilapia to S. iniae infection challenge and antibody production against the same bacterium. (C) 2013 Elsevier B.V. All rights reserved. C1 [Guimaraes, I. G.] Univ Fed Goias, Dept Zootecnia, BR-75801615 Jatai, Go, Brazil. [Lim, C.; Yildirim-Aksoy, M.; Klesius, P. H.] USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. [Li, M. H.] Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. RP Guimaraes, IG (reprint author), Univ Fed Goias, Dept Zootecnia, BR-75801615 Jatai, Go, Brazil. EM igoguimaraes@jatai.ufg.br FU Coordination of the Improvement of Higher Level Education (CAPES, Brazil); Collaborative program from Brazilian students to study abroad FX The first author thanks the Coordination of the Improvement of Higher Level Education (CAPES, Brazil) for the scholarship to Dr. Guimaraes, provided by the Collaborative program from Brazilian students to study abroad. We also thank Rashida Eljack, Biological Technician, Aquatic Animal Health Research Unit, USDA-ARS, Auburn, Alabama, for the technical assistance in this project. NR 49 TC 8 Z9 9 U1 4 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0377-8401 EI 1873-2216 J9 ANIM FEED SCI TECH JI Anim. Feed Sci. Technol. PD FEB PY 2014 VL 188 BP 126 EP 136 DI 10.1016/j.anifeedsci.2013.12.003 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA AB3BM UT WOS:000331666300014 ER PT J AU Kurpad, AV Anand, P Dwarkanath, P Hsu, JW Thomas, T Devi, S Thomas, A Mhaskar, R Jahoor, F AF Kurpad, Anura V. Anand, Pauline Dwarkanath, Pratibha Hsu, Jean W. Thomas, Tinku Devi, Santa Thomas, Annamma Mhaskar, Rita Jahoor, Farook TI Whole body methionine kinetics, transmethylation, transulfuration and remethylation during pregnancy SO CLINICAL NUTRITION LA English DT Article DE Pregnancy; Transmethylation; Remethylation; Methionine kinetics; Vitamin B-12 ID AMINO-ACID-METABOLISM; METHYLMALONIC ACID; TOTAL HOMOCYSTEINE; PROTEIN; LEUCINE; MASS; HUMANS; ENERGY; PLASMA; IMPACT AB Background & aims: There is evidence from a study of pregnant American women that methionine transmethylation (TM) and remethylation (RM) rates increases and transulfuration (TS) decreases as pregnancy progresses from trimester 1 to 3. To determine whether pregnant Indian women can make this adaptation successfully, methionine kinetics, TS, TM, and RM were measured in Indian women in early and late pregnancy. Methods: Measurements were made in the postabsorptive and fed states in the 1st and 3rd trimesters of pregnancy by infusing 1-C-13,H-2(3)-methionine in 24 women, 12 with low (<= 150 pmol L-1) and 12 with normal (>= 200 pmol L-1) vitamin B-12 status at recruitment. Results: From trimester I to 3, except RM which decreased significantly, there was no change in any weight-specific methionine kinetic parameter. When expressed per whole body, methionine flux from protein breakdown increased significantly from trimester 1 to 3 in the fed and postabsorptive states. Flux to protein synthesis also increased significantly in the fed state. Rates of TM, TS and RM did not change, regardless of vitamin B-12 status at recruitment. Protein and methionine intakes correlated with TM and RM rates and the change in RM from trimester 1 to 3 correlated with the change in dietary protein intake. Conclusion: These results suggest that methionine flux and its utilization for protein synthesis increases in Indian women as pregnancy progresses from trimester 1 to 3. TM and RM rates do not increase however, possibly because of inadequate protein intake and not because of vitamin B-12 deficiency at trimester 1. (C) 2013 Elsevier B.V. and NIPR. All rights reserved. C1 [Kurpad, Anura V.; Anand, Pauline; Dwarkanath, Pratibha; Thomas, Tinku; Devi, Santa] St Johns Natl Acad Hlth Sci, St Johns Res Inst, Div Nutr, Bangalore 560034, Karnataka, India. [Hsu, Jean W.; Jahoor, Farook] USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Thomas, Annamma; Mhaskar, Rita] St Johns Med Coll Hosp, Dept Obstet & Gynecol, Bangalore 560034, Karnataka, India. RP Kurpad, AV (reprint author), St Johns Natl Acad Hlth Sci, St Johns Res Inst, Div Nutr, Bangalore 560034, Karnataka, India. EM a.kurpad@sjri.res.in; paulineanand@gmail.com; pratibha@sjri.res.in; jeanweih@bcm.edu; tinku.sarah@sjri.res.in; sarita@sjri.res.in; rejiann@dataone.in; ritamhaskar@yahoo.com; fjahoor@bcm.tmc.edu FU Department of Biotechnology, Government of India; U.S. Department of Agriculture, Agricultural Research Service [58-6250-6001] FX Authors acknowledge the financial support provided by the Department of Biotechnology, Government of India, as well as the federal funds from the U.S. Department of Agriculture, Agricultural Research Service under Cooperative Agreement Number 58-6250-6001. We greatly appreciate the assistance of Nancy Nanditha, Jacintha Kumari in the collection and entry of data. We thank the pregnant women for their participation in this study and the doctors, nurses and laboratory technicians who made this study possible. NR 26 TC 2 Z9 2 U1 1 U2 7 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND SN 0261-5614 EI 1532-1983 J9 CLIN NUTR JI Clin. Nutr. PD FEB PY 2014 VL 33 IS 1 BP 122 EP 129 DI 10.1016/j.clnu.2012.12.016 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AB3JA UT WOS:000331685900017 PM 23356955 ER PT J AU Momany, F Schnupf, U AF Momany, Frank Schnupf, Udo TI DFT optimization and DFT-MD studies of glucose, ten explicit water molecules enclosed by an implicit solvent, COSMO SO COMPUTATIONAL AND THEORETICAL CHEMISTRY LA English DT Article DE Glucose; Molecular optimization; Molecular dynamics; DFT; DFT-MD; Explicit and implicit solvation ID BETA-D-GLUCOPYRANOSE; ALPHA-D-GLUCOPYRANOSE; AQUEOUS CARBOHYDRATE SOLUTIONS; DYNAMICS SIMULATIONS; GAS-PHASE; DIELECTRIC-RELAXATION; NEUTRON-DIFFRACTION; RELATIVE STABILITY; AB-INITIO; STEREOCHEMICAL ASPECTS AB DFT optimization and DFT-MD studies are carried out on alpha/beta-glucose surrounded by ten explicit water molecules and the glucose/water super-molecule completely enclosed by an implicit solvation model, COSMO. A set of twenty one starting configurations of the explicit waters were first optimized empirically with the AMB06C/TIP3P force field, and further optimized using a reduced basis set (B3LYP/4-31G) on the sugar carbon atoms and the larger B3LYP/6-31+G* level on all other atoms. Glucose hydroxymethyl conformations included gg, gt, and tg forms, with the hydroxyl group's conformations counter clockwise, 'r', and clockwise, 'c', forms. The empirical force field optimized configurations were in general agreement with the DFT results in hydrogen bonding distances from glucose to water molecules, but not in such good agreement in water molecule orientation, a result of lack of lone-pair orientation within the TIP3P force field. The individual energies of all components of the super-molecule complex (i.e. water water, water glucose), with and without COSMO, allows analysis of the hydration and interaction energies. Examination of the hydroxyl and hydroxymethyl low energy conformations suggests that the hydrated configurations with gg-r and gg-c conformers are preferred in water. OFT-MD is carried out on all configurations leading to understanding of the lifetimes of water molecule contact with glucose. The results suggest that no water molecule remains at any one specific site around glucose for a significant time, water molecules migrating off and moving around the molecule within several picoseconds. Published by Elsevier B.V. C1 [Momany, Frank] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Schnupf, Udo] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. RP Momany, F (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM frank.momany@ars.usda.gov RI Schnupf, Udo/H-4703-2016 OI Schnupf, Udo/0000-0002-1457-1985 NR 93 TC 4 Z9 4 U1 2 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2210-271X EI 1872-7999 J9 COMPUT THEOR CHEM JI Comput. Theor. Chem. PD FEB 1 PY 2014 VL 1029 BP 57 EP 67 DI 10.1016/j.comptc.2013.12.007 PG 11 WC Chemistry, Physical SC Chemistry GA AB3FI UT WOS:000331676300008 ER PT J AU Cetin, AE Pearson, TC Sevimli, RA AF Cetin, A. Enis Pearson, Tom C. Sevimli, R. Akin TI System for removing shell pieces from hazelnut kernels using impact vibration analysis SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Impact vibration analysis; Line spectral frequencies; Mel-cepstral feature; Hazelnuts ID DAMAGED WHEAT KERNELS; TRANSMITTANCE IMAGES; IDENTIFICATION; ACOUSTICS AB A system for removing shell pieces from hazelnut kernels using impact vibration analysis was developed in which nuts are dropped onto a steel plate and the vibration signals are captured and analyzed. The mel-cepstral feature parameters, line spectral frequency values, and Fourier-domain Lebesgue features were extracted from the vibration signals. The best experimental results were obtained using the mel-cepstral feature parameters. The feature parameters were classified using a support vector machine (SVM), which was trained a priori using a manually classified dataset. An average recognition rate of 98.2% was achieved. An important feature of the method is that it is easily trainable, enabling it to be applicable to other nuts, including walnuts and pistachio nuts. In addition, the system can be implemented in real time. (C) 2013 Elsevier B.V. All rights reserved. C1 [Cetin, A. Enis; Sevimli, R. Akin] Bilkent Univ, Dept Elect & Elect Engn, TR-06800 Ankara, Turkey. [Pearson, Tom C.] ARS, USDA, CGAHR, Manhattan, KS 66502 USA. RP Sevimli, RA (reprint author), Bilkent Univ, Dept Elect & Elect Engn, TR-06800 Ankara, Turkey. EM sevimli@ee.bilkent.edu.tr NR 20 TC 1 Z9 1 U1 0 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD FEB PY 2014 VL 101 BP 11 EP 16 DI 10.1016/j.compag.2013.11.010 PG 6 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA AB3JB UT WOS:000331686000002 ER PT J AU Yu, PC Li, CY Takeda, F Krewer, G Rains, G Hamrita, T AF Yu, Pengcheng Li, Changying Takeda, Fumiomi Krewer, Gerard Rains, Glen Hamrita, Takoi TI Measurement of mechanical impacts created by rotary, slapper, and sway blueberry mechanical harvesters SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Bruise; Accelerometer; Instrumented sphere; Sensing; Highbush blueberry AB Blueberry mechanical harvesters cause bruise damage to the fruit. The goal of this study was to test a custom-made sensor (berry impact recording device) to measure the quality and magnitude of mechanical impacts created by three major types of commercial blueberry mechanical harvesters (rotary, slapper, and sway). The sensor was mounted on blueberry bushes (cultivated) and harvested at standard operating conditions such that the sensor was detached and experienced the impact forces typically found during a mechanical harvesting process. The data collected by the sensor revealed that the slapper and sway harvesters generated not only larger number but also higher magnitude impacts than the rotary. Our analyses suggest that these disparities were mostly caused by different agitating mechanisms, contacting surface materials, and designs between the three harvesters. Results indicated that most impacts lasted 5-7 ms in all three harvesters. The distribution of the impacts showed that 90% of impacts from the rotary were less than 190 g and 90% of impacts from the slapper and sway were less than 250g. Corresponding measures were identified to reduce potential bruise damage in the harvesters. The information could be useful to select harvesters that create the least impacts and to improve current mechanical harvester designs. (C) 2014 Elsevier B.V. All rights reserved. C1 [Yu, Pengcheng; Li, Changying] Univ Georgia, Coll Engn, Athens, GA 30602 USA. [Takeda, Fumiomi] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Krewer, Gerard] Univ Georgia, Dept Hort, Tifton, GA 31793 USA. [Rains, Glen] Univ Georgia, Dept Entomol, Tifton, GA 31793 USA. [Hamrita, Takoi] Univ Georgia, Coll Engn, Athens, GA 30602 USA. RP Li, CY (reprint author), Univ Georgia, Coll Engn, 712F Boyd Bldg, Athens, GA 30602 USA. EM cyli@uga.edu FU USDA National Institute of Food and Agriculture Specialty Crop Research Initiative [2008-51180-19579] FX This project was funded by the USDA National Institute of Food and Agriculture Specialty Crop Research Initiative (Award No. 2008-51180-19579). Authors would like to thank Mr. Gary Burnham and Tim Rutland's technical support. We also appreciate the assistance provided by Mr. John Ed Smith and Mr. Robert D. Stanaland during the field tests. The Triple R. Farm, the Allen Blueberry Farm, and the DHL farm provided mechanical harvesters and blueberry bushes for the field test, for which we are grateful. We also thank Oxbo International Inc. for leasing a rotary harvester for preliminary tests on the early stage of this study. NR 18 TC 3 Z9 3 U1 3 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD FEB PY 2014 VL 101 BP 84 EP 92 DI 10.1016/j.compag.2013.12.001 PG 9 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA AB3JB UT WOS:000331686000011 ER PT J AU Brown, JP Goetz, SJ Ahearn, MC Liang, CL AF Brown, Jason P. Goetz, Stephan J. Ahearn, Mary C. Liang, Chyi-lyi (Kathleen) TI Linkages Between Community-Focused Agriculture, Farm Sales, and Regional Growth SO ECONOMIC DEVELOPMENT QUARTERLY LA English DT Article DE farm structure; community-focused agriculture; regional growth ID ECONOMIC-GROWTH; UNITED-STATES; US; AMENITIES; PATTERNS; QUALITY; SIZE AB Community-focused agriculture has been heralded as a development strategy to induce local economic growth. This study examines county-level linkages between community-focused agriculture and growth in total agricultural sales and economic growth more broadly. Using Census of Agriculture data, regional growth models are estimated on real personal income per capita change between 2002 and 2007. We find no association between community-focused agriculture and growth in total agricultural sales at the national level, but do in some regions of the United States. A $1 increase in farm sales led to an annualized increase of $0.04 in county personal income. With few exceptions, community-focused agriculture did not make significant contributions to economic growth in the time period analyzed. C1 [Brown, Jason P.] Fed Reserve Bank Kansas City, Kansas City, MO 64198 USA. [Goetz, Stephan J.] Penn State Univ, Northeast Reg Ctr Rural Dev, University Pk, PA 16802 USA. [Ahearn, Mary C.] USDA, ERS, Washington, DC 20250 USA. [Liang, Chyi-lyi (Kathleen)] Univ Vermont, Dept Community Dev & Appl Econ, Burlington, VT USA. RP Brown, JP (reprint author), Fed Reserve Bank Kansas City, 1 Mem Dr, Kansas City, MO 64198 USA. EM jason.brown@kc.frb.org NR 36 TC 5 Z9 5 U1 2 U2 10 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0891-2424 EI 1552-3543 J9 ECON DEV Q JI Econ. Dev. Q. PD FEB PY 2014 VL 28 IS 1 SI SI BP 5 EP 16 DI 10.1177/0891242413506610 PG 12 WC Economics; Planning & Development; Urban Studies SC Business & Economics; Public Administration; Urban Studies GA AB2IQ UT WOS:000331616800002 ER PT J AU Pender, JL Weber, JG Brown, JP AF Pender, John L. Weber, Jeremy G. Brown, Jason P. TI Sustainable Rural Development and Wealth Creation Five Observations Based on Emerging Energy Opportunities SO ECONOMIC DEVELOPMENT QUARTERLY LA English DT Article DE community development; industry; wealth creation; sustainability; rural development; industry studies ID WIND POWER DEVELOPMENT; NATURAL-GAS; ECONOMIC-IMPACTS; ETHANOL PLANTS; LAND-USE; US; GROWTH; EMISSIONS; FRAMEWORK; INDUSTRY AB In this article, the authors argue that better data and research on rural wealth creation are greatly needed and present a conceptual framework to help guide such research. The authors then discuss five observations about rural wealth creation, based on examples drawn from the recent literature on emerging energy industries in rural America. The cases show that the types of data needed to draw conclusions about wealth effects of new development are highly contextual. The framework can help researchers think about the types of data needed to assess policy. C1 [Pender, John L.; Weber, Jeremy G.] USDA, ERS, Resource & Rural Econ Div, Washington, DC 20250 USA. [Brown, Jason P.] Fed Reserve Bank Kansas City, Kansas City, MO USA. RP Pender, JL (reprint author), USDA, ERS, 1400 Independence Ave,SW,Mailstop 1800, Washington, DC 20250 USA. EM jpender@ers.usda.gov NR 83 TC 2 Z9 2 U1 3 U2 14 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0891-2424 EI 1552-3543 J9 ECON DEV Q JI Econ. Dev. Q. PD FEB PY 2014 VL 28 IS 1 SI SI BP 73 EP 86 DI 10.1177/0891242413513327 PG 14 WC Economics; Planning & Development; Urban Studies SC Business & Economics; Public Administration; Urban Studies GA AB2IQ UT WOS:000331616800007 ER PT J AU Best, ML Welsh, HH AF Best, Michael L. Welsh, Hartwell H., Jr. TI The trophic role of a forest salamander: impacts on invertebrates, leaf litter retention, and the humification process SO ECOSPHERE LA English DT Article DE ecological service; Ensatina eschscholtzii; humification; invertebrate predation; leaf litter retention; northern California; Plethodontidae; trophic relationships; woodland salamander ID BROOK EXPERIMENTAL FOREST; SOIL CARBON; TERRESTRIAL SALAMANDER; NEW-HAMPSHIRE; POPULATIONS; BIOMASS; SEQUESTRATION; DECOMPOSITION; BIODIVERSITY; LIZARD AB Woodland (Plethodontid) salamanders are the most abundant vertebrates in North American forests, functioning as predators on invertebrates and prey for higher trophic levels. We investigated the role of Ensatina (Ensatina eschscholtzii) in regulating invertebrate numbers and leaf litter retention in a northern California forest. Our objective was to examine how salamander predation on invertebrates affects leaf litter retention and the amount available for soil-building and carbon capture at the litter-soil interface. We used field enclosures to quantify the effects of Ensatina on invertebrates and litter retention over two wet seasons, using moisture as a covariate. In the first year Ensatina reduced Coleoptera (beetles) and Diptera (flies) larvae >2 mm, adult Coleoptera, Collembola (springtails), and Formicidae (ants), and increased Oribatid mites, larvae <2 mm (Diptera and Coleoptera), Diplopoda (millipedes), and Aranaea (spiders) <2 mm by reducing their competitors and predators. A single Ensatina in a 1.5m(2) enclosure increased litter retention by 13.3% +/- 3.6% (mean +/- SE) compared to controls, facilitating the capture of 200 kg/ha of carbon. At a similar density range-wide this would equate to 72.3 metric tons/yr of carbon in one season potentially sequestered in forest soil rather than entering the atmosphere. In the second year invertebrate densities doubled in response to early rains such that while salamanders reduced the numbers of the same taxa, effect sizes were lower compared to year one, producing biological effects that failed to achieve statistical significance. However, three taxa did significantly increase in year two (Annelida [worms], Psocoptera [barklice], and Chelonethida [pseudoscorpions]). Litter retention in year two was greater on treatment plots by 5.6% +/- 4.6%; however, high variability across plots precluded statistical significance. Ensatina suppressed some invertebrate taxa, released others, increased leaf litter retention, and facilitated greater carbon capture in both years; however, the strength of the effects were modulated by the bottom-up effects of the timing and amount of precipitation in year two. C1 [Best, Michael L.] Humboldt State Univ, Dept Wildlife, Arcata, CA 95521 USA. [Welsh, Hartwell H., Jr.] USDA Forest Serv, Pacific Southwest Res Stn, Redwood Sci Lab, Arcata, CA 95521 USA. RP Best, ML (reprint author), Humboldt State Univ, Dept Wildlife, Arcata, CA 95521 USA. EM hwelsh@fs.fed.us FU USDA Forest Service; Pacific Southwest Research Station; Reptile and Amphibian Conservancy FX This research was conducted in partial fulfillment of the Master's degree program in the Department of Wildlife at Humboldt State University by M. Best. We thank Jim Baldwin at the USDA Forest Service, Pacific Southwest Research Station for his invaluable assistance with the statistical analysis. We thank the Mattole Institute for providing the field site and logistical support. We thank two anonymous reviewers for comments that greatly improved this paper. Funding was provided by the USDA Forest Service, Pacific Southwest Research Station, and the Reptile and Amphibian Conservancy. NR 66 TC 24 Z9 25 U1 7 U2 64 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD FEB PY 2014 VL 5 IS 2 AR 16 DI 10.1890/ES13-00302.1 PG 19 WC Ecology SC Environmental Sciences & Ecology GA AB4UV UT WOS:000331786300005 ER PT J AU Elder, T AF Elder, Thomas TI Bond Dissociation Enthalpies of a Pinoresinol Lignin Model Compound SO ENERGY & FUELS LA English DT Article ID PHENETHYL PHENYL ETHERS; PYROLYTIC CLEAVAGE MECHANISMS; CHAIN HYDROXYL-GROUPS; ALPHA/BETA-SELECTIVITIES; COMPUTATIONAL PREDICTION; PHENOLIC-COMPOUNDS; PRIMARY TAR; DIMERS; LINKAGES AB The pinoresinol unit is one of the principal interunit linkages in lignin. As such, its chemistry and properties are of major importance in understanding the behavior or the polymer. This work examines the homolytic cleavage of the pinoresinol system, representing the initial step in thermal degradation. The bond dissociation enthalpy of this reaction has been evaluated using M06-2X density functional calculations. Products that allow for extensive electron delocalization are energetically favored. Calculations on subsequent reactions reveal a preference of intermediates with two unpaired electrons over a proposed four unpaired electron structure. C1 USFS, Southern Res Stn, USDA, Pineville, LA 71360 USA. RP Elder, T (reprint author), USFS, Southern Res Stn, USDA, Pineville, LA 71360 USA. EM telder@fs.fed.us NR 40 TC 11 Z9 11 U1 1 U2 31 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD FEB PY 2014 VL 28 IS 2 BP 1175 EP 1182 DI 10.1021/ef402310h PG 8 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA AB5XK UT WOS:000331861800051 ER PT J AU Andrade, NA Centofanti, T McConnell, LL Hapeman, CJ Torrents, A Nguyen, A Beyer, WN Chaney, RL Novak, JM Anderson, MO Cantrell, KB AF Andrade, Natasha A. Centofanti, Tiziana McConnell, Laura L. Hapeman, Cathleen J. Torrents, Alba Anh Nguyen Beyer, W. Nelson Chaney, Rufus L. Novak, Jeffrey M. Anderson, Marya O. Cantrell, Keri B. TI Utilizing thin-film solid-phase extraction to assess the effect of organic carbon amendments on the bioavailability of DDT and dieldrin to earthworms SO ENVIRONMENTAL POLLUTION LA English DT Article DE Bioavailability; DDT; Soil; Earthworms; Thin-film solid-phase extraction (TF-SPE) ID CONTAMINATED SEDIMENT; SOIL; CHEMICALS; ACCUMULATION; BIOCHAR; IMPACT AB Improved approaches are needed to assess bioavailability of hydrophobic organic compounds in contaminated soils. Performance of thin-film solid-phase extraction (TF-SPE) using vials coated with ethylene vinyl acetate was compared to earthworm bioassay (Lumbricus terrestris). A DDT and dieldrin contaminated soil was amended with four organic carbon materials to assess the change in bioavailability. Addition of organic carbon significantly lowered bioavailability for all compounds except for 4,4'-DDT. Equilibrium concentrations of compounds in the polymer were correlated with uptake by earthworms after 48d exposure (R-2 = 0.97; p < 0.001), indicating TF-SPE provided an accurate uptake simulation. Bioavailability of residues in soil was compared with a spiked soil aged for 90d in laboratory. Dieldrin and DDX were respectively 18% and 11% less bioavailable in contaminated soil relative to spiked soil despite >40yr of aging. Results show that TF-SPE can be useful in examining potential risks associated with contaminated soils and to test effectiveness of remediation efforts. Published by Elsevier Ltd. C1 [Andrade, Natasha A.; Centofanti, Tiziana; Torrents, Alba; Anderson, Marya O.] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [McConnell, Laura L.; Hapeman, Cathleen J.; Anh Nguyen; Chaney, Rufus L.] ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Beyer, W. Nelson] US Geol Survey, Patuxent Wildlife Res Ctr, Beltsville, MD 20705 USA. [Novak, Jeffrey M.; Cantrell, Keri B.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC USA. RP McConnell, LL (reprint author), ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM laura.mcconnell@ars.usda.gov NR 25 TC 4 Z9 5 U1 3 U2 40 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD FEB PY 2014 VL 185 BP 307 EP 313 DI 10.1016/j.envpol.2013.11.008 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA AB3DW UT WOS:000331672500037 PM 24316068 ER PT J AU Bernstein, PS Johnson, EJ Neuringer, M Schalch, W Schierle, J AF Bernstein, P. S. Johnson, E. J. Neuringer, M. Schalch, W. Schierle, J. TI Comment on: What is meso-zeaxanthin, and where does it come from? SO EYE LA English DT Letter ID OCULAR-TISSUES; CAROTENOIDS; LUTEIN; TRANSFORMATIONS; SUPPLEMENTATION C1 [Bernstein, P. S.] Univ Utah, Sch Med, Moran Eye Ctr, Salt Lake City, UT 84112 USA. [Johnson, E. J.] Tufts Univ, Carotenoids & Nutr Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Neuringer, M.] Oregon Hlth & Sci Univ, Portland, OR 97201 USA. [Schalch, W.; Schierle, J.] DSM Nutrit Prod, Basel, Switzerland. RP Bernstein, PS (reprint author), Univ Utah, Sch Med, Moran Eye Ctr, Salt Lake City, UT 84112 USA. EM elizabeth.johnson@tufts.edu FU NEI NIH HHS [R01 EY011600] NR 9 TC 5 Z9 5 U1 0 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0950-222X EI 1476-5454 J9 EYE JI Eye PD FEB PY 2014 VL 28 IS 2 BP 240 EP 242 DI 10.1038/eye.2013.257 PG 3 WC Ophthalmology SC Ophthalmology GA AB2IN UT WOS:000331616500024 PM 24310237 ER PT J AU Nayak, B Berrios, JD Tang, J AF Nayak, Balunkeswar Berrios, Jose De J. Tang, Juming TI Impact of food processing on the glycemic index (GI) of potato products SO FOOD RESEARCH INTERNATIONAL LA English DT Review DE Glycemic index; Glycemic load; Potato; Baking; Boiling; Microwave cooking; Roasting; Extrusion ID HEAT-MOISTURE TREATMENT; TYPE-2 DIABETIC-PATIENTS; AMYLOSE-LIPID COMPLEXES; IN-VITRO DIGESTIBILITY; RESISTANT STARCH; PHYSICOCHEMICAL PROPERTIES; ENDOMETRIAL CANCER; PLASMA-GLUCOSE; DIETARY FIBER; MIXED MEALS AB Potatoes are one of the most popular carbohydrate foods in industrialized and some developing countries. However, contradicting arguments and misconceptions on potatoes as a high glycemic index (GI) food is directly affecting potato consumption during the past years. Potato varieties, maturity level, starch structure, food processing techniques and composition of the meal contribute to the Cl of potatoes. Domestic boiling, baking, microwave cooking, oven cooking, extrusion and frying result in different degrees of gelatinization, and the crystallinity of starch in potato. French fried potatoes contain more resistant starch whereas boiled and mashed potatoes contribute to significant digestible starch. Extrusion processing conditions could affect the starch physicochemical structure and resulting nutritional value. Extrusion cooking makes more gelatinized starch than conventional cooking methods. Cooling or storing after processing of potatoes significantly reduces the GI due to retrogradation of starch molecules. This review provides a brief idea about the glycemic index, glycemic load, and their importance to human diseases, and detail information on the effect of food cooking methods on the glycemic index of potatoes. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Nayak, Balunkeswar] Univ Maine, Sch Food & Agr, Orono, ME 04469 USA. [Berrios, Jose De J.] USDA ARS, WRRC, Processed Foods Res Unit, Albany, CA 94710 USA. [Tang, Juming] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. RP Nayak, B (reprint author), Univ Maine, Sch Food & Agr, Orono, ME 04469 USA. EM Balunkeswar.nayak@maine.edu RI Tang, Juming/A-4027-2009; Nayak, Balunkeswar/P-7935-2014 OI Tang, Juming/0000-0001-9449-1004; Nayak, Balunkeswar/0000-0001-5831-5193 NR 121 TC 8 Z9 8 U1 9 U2 112 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 EI 1873-7145 J9 FOOD RES INT JI Food Res. Int. PD FEB PY 2014 VL 56 BP 35 EP 46 DI 10.1016/j.foodres.2013.12.020 PG 12 WC Food Science & Technology SC Food Science & Technology GA AB5TK UT WOS:000331851400005 ER PT J AU Zhu, S Li, Y Li, Z Ma, CY Lou, ZX Yokoyama, W Wang, HX AF Zhu, Song Li, Yue Li, Zhe Ma, Chaoyang Lou, Zaixiang Yokoyama, Wallace Wang, Hongxin TI Lipase-catalyzed synthesis of acetylated EGCG and antioxidant properties of the acetylated derivatives SO FOOD RESEARCH INTERNATIONAL LA English DT Article DE EGCG; Acetylation; Lipase; Organic media; Antioxidant activity ID GREEN TEA POLYPHENOLS; REGIOSELECTIVE ACYLATION; IN-VITRO; BIOAVAILABILITY; TOXICOLOGY; STABILITY; CATECHINS; ACETATE; OIL AB (-)-Epigallocatechin-3-O-gallate (EGCG) acetylated derivatives were prepared by lipase catalyzed acylation of EGCG with vinyl acetate to improve its lipophilicity and expand its application in lipophilic media. The immobilized lipase, Lipozyme RM IM, was found to be the optimum catalyst. The optimized conditions were as follows, 1:1 of the molar ratio of EGCG to vinyl acetate, 2.0% (w/w of both substrates) of enzyme amount, and 84.5% conversion was obtained after 8 h reaction at 40 degrees C in acetonitrile. The presence of mono-, di- and tri-acetylated derivatives in acetylated EGCG were confirmed by LC-MS-MS and the tri-acetylated EGCG was identified as 5',3 '',5 ''-3-O-acetyl-EGCG by NMR. Their enhanced lipophilicity was confirmed by octanol-water partition coefficient. The antioxidant activity of the acetylated EGCG derivatives were superior to butylated hydroxytoluene (BHT), tert-butyl hydroquinone (TBHQ) and EGCG as determined by peroxide values (POVs) in sunflower oil as well as by the p-anisidine method. Acetylated EGCG exhibited the highest 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (IC50 of 0.09 mg/mL) compared to EGCG, BHT and TBHQ Acetylated EGCG might be used as a potent antioxidant for controlling oxidation of sunflower oil. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Zhu, Song] Bright Dairy & Food Co Ltd, Ctr Technol, State Key Lab Dairy Biotechnol, Shanghai 200436, Peoples R China. [Zhu, Song; Li, Yue; Li, Zhe; Ma, Chaoyang; Lou, Zaixiang; Wang, Hongxin] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China. [Yokoyama, Wallace] USDA, ARS, Albany, CA 94710 USA. RP Wang, HX (reprint author), Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China. EM zhusong@jiangnan.edu.cn FU National Nature Science Foundation of China [31071601]; National Twelfth-Five Year Research Program of China [2012BAD33B05]; Priority Academic Program Development of Jiangsu Higher Education Institutions; [SKLDB2012-004] FX We gratefully acknowledge the financial support of the National Nature Science Foundation of China (No. 31071601), the National Twelfth-Five Year Research Program of China (No. 2012BAD33B05) and SKLDB2012-004. This research also was funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. NR 33 TC 8 Z9 10 U1 5 U2 43 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 EI 1873-7145 J9 FOOD RES INT JI Food Res. Int. PD FEB PY 2014 VL 56 BP 279 EP 286 DI 10.1016/j.foodres.2013.10.026 PG 8 WC Food Science & Technology SC Food Science & Technology GA AB5TK UT WOS:000331851400034 ER PT J AU Reidy, JL Thompson, FR Kendrick, SW AF Reidy, Jennifer L. Thompson, Frank R., III Kendrick, Sarah W. TI Breeding bird response to habitat and landscape factors across a gradient of savanna, woodland, and forest in the Missouri Ozarks SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Bird density; Canopy cover; Distance-based models; Landscape forest cover; Prescribed burns; Tree density ID OAK SAVANNAS; AVIAN RESPONSE; COMMUNITIES; ABUNDANCE; FIRE; CONSERVATION; RESTORATION; POPULATIONS; DISTURBANCE; VEGETATION AB Savanna and woodland were once common in the Midwest, but land use changes have led to increasing scarcity of these communities. These transitional habitats are being restored across the Midwest, but few studies have evaluated the response of wildlife to restoration or the vegetative gradient created by management. We conducted point counts for 25 songbirds at sites undergoing savanna or woodland restoration and nearby non-managed forest sites across the Ozark Highlands of Missouri during the 2009-2011 breeding seasons; these sites represented a gradient of canopy cover and tree density from savanna to woodland to forest. We estimated density of 17 species with >= 50 detections using distance-based models, which adjust estimates by the detection probability. Bird densities were more strongly related to habitat structure, fire history, and landscape composition than simply whether a site was managed or non-managed. Mature forest species such as Acadian Flycatcher (Empidonax virescens), Northen Parula (Setophaga americana), Red-eyed Vireo (Vireo olivaceous), and Worm-eating Warbler (Helmitheros vermivorum) were generally more abundant at points with more trees, higher canopy cover, lower shrub density, and less frequent or no fire in the 20 years prior to surveys. Woodland generalists such as Blue-gray Gnatcatcher (Polioptila caerulea), Eastern Wood-Pewee (Contopus virens), Great Crested Flycatcher (Myiarchus crinitus), Summer Tanager (Piranga rubra), and Yellow-billed Cuckoo (Coccyzus americanus) were generally more abundant at points with less landscape forest cover (10-km scale), more large and fewer small trees, intermediate to high canopy cover, lower shrub density, and recent or frequent fire. Early-successional species such as Eastern Towhee (Pipilo erythrophthalmus), Field Sparrow (Spizella pusilla), Indigo Bunting (Passerina cyanea), Kentucky Warbler (Geothlypis formosa), Prairie Warbler (Setophaga discolor), White-eyed Vireo (Vireo griseus) and Yellow-breasted Chat (Icteria virens) were generally more abundant at points with lower canopy cover, recent or frequent fire, and higher shrub density. Brown-headed Cowbirds (Molothrus ater) were more abundant at points with intermediate landscape forest cover and lower canopy cover. Restored sites provided breeding habitat for woodland generalists and early-successional species of conservation concern; however, managed landscapes with more open canopy and herbaceous ground cover may be required for species more indicative of open savannas. (C) 2013 Elsevier B.V. All rights reserved. C1 [Reidy, Jennifer L.; Kendrick, Sarah W.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. [Thompson, Frank R., III] US Forest Serv, USDA, No Res Stn, Columbia, MO 65211 USA. RP Reidy, JL (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM jennifer.reidy@gmail.com FU U.S. Department of Agriculture Forest Service Northern Research Station; Mark Twain National Forest; Missouri Department of Natural Resources; Missouri Department of Conservation; Nature Conservancy FX We are grateful to A. Vaughan, K. McCarty, T. Nigh, D. Ladd, and P. Nelson for assistance with site selection and other support. We thank W. Dijak for calculating landscape metrics and W.A. Cox, J. Fitzgerald, D.T. Jones-Farrand, and R. Stanton for reviewing earlier drafts of this manuscript. We thank S. Caird, R. Evans, and M. Wickens for assisting in data collection. Funding or other support was provided by U.S. Department of Agriculture Forest Service Northern Research Station and Mark Twain National Forest, the Missouri Department of Natural Resources, the Missouri Department of Conservation, and The Nature Conservancy. NR 36 TC 12 Z9 13 U1 5 U2 73 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2014 VL 313 BP 34 EP 46 DI 10.1016/j.foreco.2013.10.042 PG 13 WC Forestry SC Forestry GA AB3BF UT WOS:000331665600005 ER PT J AU Wei, L Marshall, JD Zhang, JW Zhou, H Powers, RF AF Wei, Liang Marshall, John D. Zhang, Jianwei Zhou, Hang Powers, Robert F. TI 3-PG simulations of young ponderosa pine plantations under varied management intensity: Why do they grow so differently? SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Stable carbon isotope ratio; Quantum yield; Model calibration; Shrub competition; Canopy closure; Tree-ring ID FOREST ECOSYSTEM MANAGEMENT; WATER-VAPOR EXCHANGE; STOMATAL CONDUCTANCE; UNDERSTORY VEGETATION; NORTHERN CALIFORNIA; CARBON-DIOXIDE; USE EFFICIENCY; PROCESS MODEL; DOUGLAS-FIR; TREE HEIGHT AB Models can be powerful tools for estimating forest productivity and guiding forest management, but their credibility and complexity are often at issue for forest managers. We parameterized a process-based forest growth model, 3-PG (Physiological Principles Predicting Growth), to simulate growth of ponderosa pine (Pinus ponderosa) plantations in Northern California. We used data collected from the "Garden of Eden" study, which was established in the 1980s to determine the effect of silvicultural treatments on plantation growth. We picked three sites representing a gradient of water availability and site productivity to run 3-PG. We modified the original linear canopy closure function to a power curve to capture observed stand dynamics in situ. We also added new functions to estimate the leaf area index and transpiration of the trees' understory competitors. These new functions restricted shrub growth with light intensity and assumed a fix ratio of shrub/tree transpiration per leaf area index. A delta C-13 submodel, which estimated the ratio of stable carbon isotopes (delta C-13) in plant tissue, played a key role in assigning values to gas-exchange parameters in the model. The resulting parameter values were similar to those fitted using sap flux. We replaced the original age modifier with tree-height based functions to reflect the decreased forest productivity as trees grew taller; tree height drove the change of maximum canopy conductance and its responsiveness to water vapor pressure deficit in the new functions. Some key parameters differed among sites, including quantum yield, maximum canopy conductance, and leaf allocation. The model successfully simulated the tree growth responses to fertilization and vegetation control at all three sites. The temporal variation of simulated shrub leaf area index was similar to the observed variation in shrub cover. These results help us to understand forest-growth responses to fertilizer and vegetation control, identify key tree and site parameters, and provide tuned model parameterizations that can predict the results of management alternatives in a changing climate. (C) 2013 Elsevier B.V. All rights reserved. C1 [Wei, Liang; Marshall, John D.] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. [Marshall, John D.] Swedish Agr Univ, Dept Forest Ecol & Management, S-90183 Umea, Sweden. [Zhang, Jianwei; Powers, Robert F.] US Forest Serv, Pacific Southwest Res Stn, USDA, Redding, CA 96002 USA. [Zhou, Hang] Univ Idaho, Dept Geog, Moscow, ID 83844 USA. RP Wei, L (reprint author), Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. EM liangwei@vandals.uidaho.edu RI Wei, Liang/K-3522-2015 OI Wei, Liang/0000-0001-8967-5036 FU Sierra Cascade Intensive Forest Management; Sierra Pacific Industries FX We thank Therese Alves, Bob Carlson, Gary Fiddler, Donald Jones, Carol Shestak, Bert Spear, David Young (all in Pacific Southwest Research Station - Redding), and others who helped install, maintain, and measure these GOE plots during the last 20 years. We also thank Allen Tedrow, and lab managers of Idaho Stable Isotopes Lab, Margaret Ward and Benjamin Miller, for processing tree ring delta13C samples. Special thanks to Richard Waring, Joe Landsberg, and Peter Sands for their insightful and supportive comments to this study. The Sierra Cascade Intensive Forest Management Research Cooperative and Sierra Pacific Industries provided partial financial support for the project. NR 61 TC 6 Z9 6 U1 2 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2014 VL 313 BP 69 EP 82 DI 10.1016/j.foreco.2013.10.035 PG 14 WC Forestry SC Forestry GA AB3BF UT WOS:000331665600009 ER PT J AU Bataineh, MM Wagner, RG Olson, MG Olson, EK AF Bataineh, Mohammad M. Wagner, Robert G. Olson, Matthew G. Olson, Elizabeth K. TI Midrotation response of ground vegetation to herbicide and precommercial thinning in the Acadian Forest of Maine, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Understory; Herbaceous; Spruce-fir; Biodiversity; Intensive management; Variance-partitioning ID LONG-TERM RESPONSE; PLANT-SPECIES-DIVERSITY; PONDEROSA PINE FOREST; UNDERSTORY VEGETATION; BOREAL FOREST; TEMPERATE FOREST; HERBACEOUS-LAYER; STRIPED MAPLE; NORTH-AMERICA; NEW-BRUNSWICK AB Silvicultural practices can directly or indirectly influence the abundance and diversity of ground vegetation, and thereby influence a vital component of forest ecosystems. As silvicultural intensity increases, understanding the effects on ground vegetation become more critical. Long-term data on the response of ground vegetation to various intensities of forest management are limited. Therefore, we used a long-term experiment to quantify how individual and combined applications of herbicide and precommercial thinning (PCT) early in stand development influenced the cover, richness, and diversity of ground vegetation at midrotation (30-40 years) in spruce-fir stands of the Acadian Region. Aerial application of different herbicides (including glyphosate, triclopyr, and phenoxys) had no long-term effect (23-33 years after treatment) on cover, richness, and diversity of ground vegetation. In contrast, PCT had a positive and long-lasting effect (up to 24 years post-treatment) on ground vegetation cover, richness, and diversity. Our results indicated that overstory structure had a larger influence on ground vegetation than overstory species composition. Thus, manipulation of stand structure and the maintenance of a variety of stand structures across the landscape are important strategies for managing the diversity of ground vegetation. We conclude that PCT, especially following herbicide application, is an effective silvicultural tool for the maintenance of ground vegetation diversity, as well as timber production. (C) 2013 Elsevier B.V. All rights reserved. C1 [Bataineh, Mohammad M.; Wagner, Robert G.] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Olson, Matthew G.; Olson, Elizabeth K.] Missouri Dept Conservat, Forest Syst Field Stn, West Plains, MO 65775 USA. RP Bataineh, MM (reprint author), Univ Maine, USDA, Cooperat Forestry Res Unit, US Forest Serv, 5755 Nutting Hall, Orono, ME 04469 USA. EM mohammad.albataineh@maine.edu FU USDA Forest Service, Northern Research Station; University of Maine's Cooperative Forestry Research Unit; Maine Agricultural and Forest Experiment Station at the University of Maine [MAFES 3332] FX Funding for this project was provided by the USDA Forest Service, Northern Research Station and the University of Maine's Cooperative Forestry Research Unit. We thank Plum Creek Timber Company Inc., for maintaining and providing access to the study site. We thank Drs. Maxwell McCormack and Michael Newton for their efforts to maintain this long-term experiment. Review of an earlier version of this paper was provided by Dr. Laura Kenefic. This work was supported by the Maine Agricultural and Forest Experiment Station at the University of Maine (MAFES 3332). NR 72 TC 1 Z9 1 U1 2 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2014 VL 313 BP 132 EP 143 DI 10.1016/j.foreco.2013.11.007 PG 12 WC Forestry SC Forestry GA AB3BF UT WOS:000331665600016 ER PT J AU Rota, CT Rumble, MA Millspaugh, JJ Lehman, CP Kesler, DC AF Rota, Christopher T. Rumble, Mark A. Millspaugh, Joshua J. Lehman, Chadwick P. Kesler, Dylan C. TI Space-use and habitat associations of Black-backed Woodpeckers (Picoides arcticus) occupying recently disturbed forests in the Black Hills, South Dakota SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Bark beetles; Mountain pine beetle; Prescribed fire; Resource selection; Wildfire ID MOUNTAIN PINE-BEETLE; HOME-RANGE SIZE; PONDEROSA PINE; RESOURCE SELECTION; BOREAL FOREST; BIRD COMMUNITIES; CONIFER FORESTS; BURNED FORESTS; FIRE SEVERITY; SIERRA-NEVADA AB Black-backed Woodpeckers (Picoides arcticus) are a disturbance-dependent species that occupy recently burned forest and mountain pine beetle (MPB) infestations. Forest management practices that reduce the amount of disturbed forest may lead to habitat loss for Black-backed Woodpeckers, which have recently been petitioned for listing under the Endangered Species Act. We studied home range size and resource selection of Black-backed Woodpeckers occupying habitat created by summer wildfire, fall prescribed fire, and MPB infestations in the Black Hills, South Dakota. We studied home range size and resource selection by attaching radio-transmitters to adult Black-backed Woodpeckers. We estimated home range size using fixed kernel density techniques (n = 28 in habitat created by summer wildfire, n = 19 in habitat created by fall prescribed fire, and n = 27 in MPB infestations). We evaluated resource selection with a random-effects discrete choice model in a Bayesian framework (n = 5 in habitat created by summer wildfire, n = 16 in habitat created by fall prescribed fire, and n = 8 in habitat created by MPB infestations). Home range size was smallest in 1-2 year post summer wildfire habitat (mean home range size = 79 ha) and 2-year post fall prescribed fire habitat (mean home range size = 143 ha). Home range size was intermediate in MPB infestations (mean home range size = 307 ha) and was greatest in 3-4 year post fire habitat (mean summer wildfire home range size = 430 hectares, mean fall prescribed fire home range size = 460 ha). The relative probability that a Black-backed Woodpecker used a tree increased with increasing diameter at breast height (DBH) and basal area and was greatest on disturbance-killed trees. These results suggest 1-2 year post summer wildfire habitat may have the greatest relative value to Black-backed Woodpeckers and that MPB infestations may be more important as post-fire habitats age. We recommend retaining patches of 1-2 year post summer wildfire habitat that are at least 200 ha by exempting portions of recently burned forest from salvage logging. This recommendation can be accomplished by exempting salvage logging in summer wildfires <200 ha and retaining at least 50% of summer wildfires >400 ha. Ideally, recently burned forest patches should be composed of >= 27 m(2) basal area/ha of trees that burned at moderate or high severity, with at least 40% of the basal area composed of trees >= 27 cm DBH. (C) 2013 Elsevier B.V. All rights reserved. C1 [Rota, Christopher T.; Millspaugh, Joshua J.; Kesler, Dylan C.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. [Rumble, Mark A.] US Forest Serv, Forest & Grassland Res Lab, Rocky Mt Res Stn, Rapid City, SD 57702 USA. [Lehman, Chadwick P.] Custer State Pk, Custer, SD 57730 USA. RP Rota, CT (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM rotact@missouri.edu OI Rota, Christopher/0000-0001-9272-4687 FU U.S. Forest Service Rocky Mountain Research Station [07-JV-11221609-211, 10-JV-11221632-178]; South Dakota Department of Game, Fish and Parks [08-CO-11221632-111, T-39-R-1]; Missouri Chapter of the Wildlife Society; University of Missouri ORG; James D. Chambers Memorial Scholarship; Judy Southern Fellowship; TransWorld Airlines Scholarship FX Funding for this project was provided by the U.S. Forest Service Rocky Mountain Research Station agreement 07-JV-11221609-211 and the National Fire Plan agreement 10-JV-11221632-178. The South Dakota Department of Game, Fish and Parks provided funding through agreement number 08-CO-11221632-111 and the Wildlife Diversity Small Grants Program. This study was also made possible through State Wildlife Grant T-39-R-1, Study #2439, provided by the South Dakota Department of Game, Fish, and Parks. We thank Eileen Dowd-Stukel, Shelly Diesch, and Casey Mehls for funding support administered through their programs at the South Dakota Department of Game, Fish, and Parks. C.T.R. was supported by the Missouri Chapter of the Wildlife Society, the University of Missouri ORG, the James D. Chambers Memorial Scholarship, the Judy Southern Fellowship, and a TransWorld Airlines Scholarship. We thank M. Clawson, J. Dacey, B. Dickerson, M. Dolan, B. Furfey, K. Hansen, S. Hardaswick, M. Immel, W. Johnson, T. Juntti, S. Kolbe, R. Kummer-Bolie, N. Magliocco, E. Margenau, H. Mason, R. Mowry, B. Prochazka, B. Rota, H. Scott, L. Schulte-Welle, E. Seckinger, and C. Smithers for field assistance. We thank B. Keller, M. Ryan, F. Thompson III, C. Wikle, and 2 anonymous reviewers for helpful insights on previous versions of this manuscript. NR 51 TC 10 Z9 13 U1 4 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2014 VL 313 BP 161 EP 168 DI 10.1016/j.foreco.2013.10.048 PG 8 WC Forestry SC Forestry GA AB3BF UT WOS:000331665600019 ER PT J AU Sturtevant, BR Miranda, BR Wolter, PT James, PMA Fortin, MJ Townsend, PA AF Sturtevant, Brian R. Miranda, Brian R. Wolter, Peter T. James, Patrick M. A. Fortin, Marie-Josee Townsend, Philip A. TI Forest recovery patterns in response to divergent disturbance regimes in the Border Lakes region of Minnesota (USA) and Ontario (Canada) SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest harvest patterns; Natural disturbance patterns; Landscape disturbance legacy; Disturbance regime; Forest recovery; Boreal forest ID LANDSAT TIME-SERIES; BOREAL FOREST; SPRUCE BUDWORM; LANDSCAPE PATTERNS; NATURAL DISTURBANCES; WESTERN OREGON; AGE STRUCTURE; COVER CHANGE; TM IMAGERY; DYNAMICS AB The persistence of landscape-scale disturbance legacies in forested ecosystems depends in part on the nature and strength of feedback among disturbances, their effects, and subsequent recovery processes such as tree regeneration and canopy closure. We investigated factors affecting forest recovery rates over a 25-year time period in a large (6 million ha) landscape where geopolitical boundaries have resulted in important land management legacies (managed forests of Minnesota, USA; managed forests of Ontario, Canada; and a large unmanaged wilderness). Stand-replacing disturbance regimes were quantified across management zones, both inside and outside a central ecoregion, using a time series of classified land cover data constructed at 5-year intervals between 1975 and 2000. The temporally variable disturbance regime of the wilderness was characterized by fine-scaled canopy disturbances punctuated by less frequent large disturbance events (i.e., fire and blow down). The comparably consistent disturbance regimes of the managed forests of Minnesota and Ontario differed primarily in the size distribution of disturbances - principally clearcut harvesting. Using logistic regression we found that a combination of time since disturbance, mapped disturbance attributes, climate, and differences among management zones affected pixel-scale probabilities of forest recovery that reflect recovery rates. We conclude that the magnitude of divergence in landscape disturbance legacies of this region will be additionally reinforced by regional variations in the human and natural disturbance regimes and their interactions with forest recovery processes. Our analyses compliment traditional plot-scale studies that investigate post-disturbance recovery by (a) examining vegetation trends across a wide range of variability and (b) quantifying the cumulative effects of disturbances as they affect recovery rates over a broad spatial extent. Our findings therefore have implications for sustainable forestry, ecosystem-based management, and landscape disturbance and succession modeling. Published by Elsevier B.V. C1 [Sturtevant, Brian R.; Miranda, Brian R.] US Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, Rhinelander, WI 54501 USA. [Wolter, Peter T.] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. [James, Patrick M. A.] Univ Montreal, Dept Sci Biol, Montreal, PQ H2V 2S9, Canada. [Fortin, Marie-Josee] Univ Toronto, Dept Ecol & Evolutionary Biol, Toronto, ON M5S 3B2, Canada. [Townsend, Philip A.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. RP Sturtevant, BR (reprint author), US Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, 5985 Hwy K Rhinelander, Rhinelander, WI 54501 USA. EM bsturtevant@fs.fed.us RI Townsend, Philip/B-5741-2008 OI Townsend, Philip/0000-0001-7003-8774 FU USDA Cooperative State Research, Education and Extension Service (CSREES) [2005-35101-16342]; US National Fire Plan FX This research was supported by the USDA Cooperative State Research, Education and Extension Service (CSREES) Managed Ecosystems program (2005-35101-16342) and the US National Fire Plan. We wish to thank Sue Lietz for technical support. John Stanovick provided valuable guidance on our statistical methods. We also thank Doug Shinneman, Brian Palik, and two anonymous reviewers for helpful comments on the manuscript. NR 83 TC 9 Z9 9 U1 4 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2014 VL 313 BP 199 EP 211 DI 10.1016/j.foreco.2013.10.039 PG 13 WC Forestry SC Forestry GA AB3BF UT WOS:000331665600023 ER PT J AU Oldfield, EE Felson, AJ Wood, SA Hallett, RA Strickland, MS Bradford, MA AF Oldfield, Emily E. Felson, Alexander J. Wood, Stephen A. Hallett, Richard A. Strickland, Michael S. Bradford, Mark A. TI Positive effects of afforestation efforts on the health of urban soils SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Afforestation; Ecosystem services; Restoration; Urban forestry ID CARBON STOCKS; ORGANIC-CARBON; RESTORATION MANAGEMENT; ECOLOGICAL KNOWLEDGE; METAANALYSIS; TEMPERATURE; MITIGATION; NITROGEN; QUALITY; FOREST AB Large-scale tree planting projects in cities are increasingly implemented as a strategy to improve the urban environment. Trees provide multiple benefits in cities, including reduction of urban temperatures, improved air quality, mitigation of storm-water run-off, and provision of wildlife habitat. How urban afforestation affects the properties and functions of urban soils, however, is largely unknown. As healthy soils are critical for vigorous tree growth, our study explores the impact of soil restoration as part of a large-scale urban afforestation project. We collected data on multiple soil variables over the first three years of the New York City Afforestation Project (NY-CAP). The study consists of 56 plots of 225 m(2) arrayed across an urban parkland in Queens, NYC. Each plot contains 56 trees made up of two (low richness) versus six (high richness) native species. The richness treatment was crossed with stand complexity (with shrubs and herbs versus without), and soil amendment (with compost versus without). We sampled soils in 2009 prior to project establishment, in 2010 following site preparation but just prior to planting, and again in 2011 one year after the 3-5 year old saplings were planted and plot treatments were put in place. We present results for the effects of site preparation on soil properties over time from baseline conditions in 2009 through the first year of afforestation in 2011. We also explore the impact of plot treatments (listed above and implemented right after our 2010 soil sampling) on soil parameters in 2011. Overall, site preparation improves soil conditions for the native tree saplings across time, with reductions in bulk density from similar to 1.4 to 0.72 g cm(-3), acidification of the soil from pH 7.36 to 7.03, a 4-fold increase in microbially-available carbon and a 1.3-times increase in microbial biomass. Furthermore, soil carbon concentrations increased by 1.33-times between 2009 and 2011. Exploring plot treatments in 2011, compost had the largest effect, with 1.23-times more microbial biomass in composted plots, more acidic pH values (6.66 versus 7.37 in non-composted plots) and increased water holding capacity (35% versus 31% in non-composted plots). The observed changes in soil physical, chemical and biological properties suggest that site preparation and management improves traits of urban soils that are critical for infiltration, decomposition, mineralization and nutrient retention. The initial trajectories of change in these soil properties provide support for the expectation that urban afforestation - and specifically the preparation of urban soils for tree planting - will improve the health of urban soils and consequently the urban environment. (C) 2013 Elsevier B.V. All rights reserved. C1 [Oldfield, Emily E.; Felson, Alexander J.; Wood, Stephen A.; Strickland, Michael S.; Bradford, Mark A.] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [Felson, Alexander J.] Yale Univ, Sch Architecture, New Haven, CT 06511 USA. [Wood, Stephen A.] Columbia Univ, Dept Ecol Evolut & Environm Biol, New York, NY 10027 USA. [Hallett, Richard A.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. [Strickland, Michael S.] Virginia Polytech Inst & State Univ, Dept Biol Sci, Blacksburg, VA 24061 USA. RP Oldfield, EE (reprint author), Yale Univ, Sch Forestry & Environm Studies, 370 Prospect St, New Haven, CT 06511 USA. EM emily.oldfield@yale.edu; alexander.felson@yale.edu; saw2177@columbia.edu; rah@unh.edu; strick77@vt.edu; mark.bradford@yale.edu RI Bradford, Mark/G-3850-2012; Strickland, Michael/B-7137-2016; Wood, Stephen/A-1928-2017 OI Bradford, Mark/0000-0002-2022-8331; Strickland, Michael/0000-0001-5349-0363; Wood, Stephen/0000-0002-9551-8165 NR 39 TC 11 Z9 13 U1 15 U2 101 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2014 VL 313 BP 266 EP 273 DI 10.1016/j.foreco.2013.11.027 PG 8 WC Forestry SC Forestry GA AB3BF UT WOS:000331665600029 ER PT J AU Flower, CE Long, LC Knight, KS Rebbeck, J Brown, JS Gonzalez-Meler, MA Whelan, CJ AF Flower, Charles E. Long, Lawrence C. Knight, Kathleen S. Rebbeck, Joanne Brown, Joel S. Gonzalez-Meler, Miguel A. Whelan, Christopher J. TI Native bark-foraging birds preferentially forage in infected ash (Fraxinus spp.) and prove effective predators of the invasive emerald ash borer (Agrilus planipennis Fairmaire) SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Bark-foraging birds; Woodpecker; Foraging; Emerald ash borer; Fraxinus; Quitting harvest rate ID GAINING ECOLOGICAL INFORMATION; MOUNTAIN PINE-BEETLE; CAVITY-NESTING BIRDS; BAYESIAN FORAGERS; NORTH-AMERICAN; PATCH-USE; WOODPECKERS; BEHAVIOR; COLEOPTERA; RESOURCES AB Inadvertently introduced into North America in the 1990s, the invasive emerald ash borer (EAB, Agrilus planipennis) has been spreading across the Great Lakes Region resulting in widespread ash tree (Fraxinus spp.) mortality. Native woodpeckers and other bark-foraging insectivores represent one of the few potential natural predators of EAB in the U.S. In this study, we combined observational and destructive tree harvesting approaches to assess bark-foraging bird predation on EAB larvae in a deciduous forest of central Ohio. Results of our observational study show that in an EAB impacted forest, bark-foraging birds forage more heavily on ash trees than non-ash trees, and that they forage preferentially on ash trees that exhibit canopy decline symptoms relative those with healthy canopies. These patterns were further supported by the destructive sampling of 46 ash trees wherein predation by bark-foragers significantly reduced tree-level EAB densities by upwards of 85%. Bark-foraging predation intensity increased with increased EAB infestation levels, with bark-foragers harvesting similar to 45% of EAB in trees with thinning canopies compared to similar to 22% in ash trees with healthy canopies. Woodpeckers harvest EAB in a density-dependent pattern that could contribute to population control. Despite bark-forager predation, LAB had a high likelihood of successfully emerging from the heavily infested ash trees (similar to 30% or 35 EAB per m(2)). Our results suggest that woodpeckers and other bark-foragers may use visual canopy decline, and perhaps other cues, to target ash trees with increased EAB densities. Moreover, our results provide insight into the indirect effects of invasive species on biotic interactions in forest ecosystems highlighting potential shifts in bark-foraging and other bird behaviors in response to a novel forest pest. Bark-foragers respond to LAB infestation and may thus potentially help regulate EAB populations and their spread in a mixed deciduous forest. We suggest that maintaining snags and nesting sites during and after forest pest outbreaks may enhance populations of bark-foraging bird species and, thus, their biological control of pest insects in temperate deciduous forests. (C) 2013 Elsevier B.V. All rights reserved. C1 [Flower, Charles E.; Brown, Joel S.; Gonzalez-Meler, Miguel A.] Univ Illinois, Dept Biol Sci Mc 066, Chicago, IL 60607 USA. [Long, Lawrence C.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Flower, Charles E.; Knight, Kathleen S.; Rebbeck, Joanne] US Forest Serv, USDA, No Res Stn, Delaware, OH 43015 USA. [Whelan, Christopher J.] Univ Illinois, Illinois Nat Hist Survey, Chicago, IL 60607 USA. RP Flower, CE (reprint author), Univ Illinois, Dept Biol Sci Mc 066, 845 West Taylor St, Chicago, IL 60607 USA. EM cflowe3@uic.edu; long.643@osu.edu; ksnight@fs.fed.us; jrebbeck@fs.fed.us; squirrel@darwiniandynamics.org; mmeler@uic.edu; virens@darwiniandynamics.org OI Gonzalez-Meler, Miquel/0000-0001-5388-7969; Whelan, Christopher/0000-0001-7511-2603 FU National Science Foundation [DGE-0549245]; University of Illinois at Chicago; U.S. National Science Foundation [DEB-0919276]; United States Forest Service Northern Research Station Civil Rights Diversity Special Project Fund FX The authors would like to thank D. Bogard and the 7th and 8th grade students in the advanced science classes for assistance processing samples and T. Fox, J. Jolliff, and K. Costilow for their field assistance. We would also like to thank the reviewers for their thoughtful and thorough comments which greatly improved the manuscript. This research was supported by the National Science Foundation Grant DGE-0549245, "Landscape Ecological and Anthropogenic Processes", the University of Illinois at Chicago (Hadley grant and Provost Fellowship), U.S. National Science Foundation DEB-0919276 to MAG-M, and the United States Forest Service Northern Research Station Civil Rights Diversity Special Project Fund. NR 65 TC 13 Z9 14 U1 21 U2 116 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2014 VL 313 BP 300 EP 306 DI 10.1016/j.foreco.2013.11.030 PG 7 WC Forestry SC Forestry GA AB3BF UT WOS:000331665600033 ER PT J AU Xie, J Chen, JQ Sun, G Chu, HS Noormets, A Ouyang, ZT John, R Wan, SQ Guan, WB AF Xie, Jing Chen, Jiquan Sun, Ge Chu, Housen Noormets, Asko Ouyang, Zutao John, Ranjeet Wan, Shiqiang Guan, Wenbin TI Long-term variability and environmental control of the carbon cycle in an oak-dominated temperate forest SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Net ecosystem exchange of carbon; Gross ecosystem productivity; Ecosystem respiration; Eddy-covariance; Seasonal and interannual variability; Temperate deciduous forest ID NET ECOSYSTEM PRODUCTIVITY; WATER-VAPOR EXCHANGE; SOIL RESPIRATION; INTERANNUAL VARIABILITY; SONIC ANEMOMETER; DIOXIDE EXCHANGE; HARDWOOD FOREST; ATMOSPHERIC CO2; USE EFFICIENCY; SEVERE DROUGHT C1 [Xie, Jing] Beijing Forestry Univ, Coll Soil & Water Conservat, Beijing 100083, Peoples R China. [Xie, Jing; Chen, Jiquan; Chu, Housen; Ouyang, Zutao; John, Ranjeet] Univ Toledo, Dept Environm Sci, Toledo, OH 43606 USA. [Chen, Jiquan] Nanjing Univ Informat Sci & Technol, Sch Appl Meteorol, Int Ctr Ecol Meteorol & Environm IceMe, Nanjing 210044, Jiangsu, Peoples R China. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Noormets, Asko] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27606 USA. [Wan, Shiqiang] Henan Univ, Coll Life Sci, Key Lab Plant Stress Biol, Kaifeng 475004, Henan, Peoples R China. [Guan, Wenbin] Beijing Forestry Univ, Coll Nat Conservat, Beijing 100083, Peoples R China. RP Chen, JQ (reprint author), Univ Toledo, Dept Environm Sci, 2801 W Bancroft St, Toledo, OH 43606 USA. EM jiquan.chen@utoledo.edu RI Wan, Shiqiang/B-5799-2009; Chen, Jiquan/D-1955-2009; John, Ranjeet/J-2082-2015; Chu, Housen/Q-6517-2016; OI John, Ranjeet/0000-0002-0150-8450; Chu, Housen/0000-0002-8131-4938; Noormets, Asko/0000-0003-2221-2111 FU IceMe at NUIST; Eastern Forest Environmental Threat Assessment Center, Southern Research Station, USDA Forest Service [03-CA-11330147-073, 04-CA-11330147-238]; China Scholarship Council [20093012]; Toledo Area Metroparks; US-China Carbon Consortium (USCCC) FX This study was funded by the IceMe at NUIST, the Eastern Forest Environmental Threat Assessment Center, Southern Research Station, USDA Forest Service (Cooperative Agreements 03-CA-11330147-073 and 04-CA-11330147-238), the China Scholarship Council (20093012), the Toledo Area Metroparks, and the US-China Carbon Consortium (USCCC), which promotes collaborative research among interested institutions in the US and China. We also thank many members of the Landscape Ecology and Ecosystem Sciences Lab at the University of Toledo for their thoughtful comments and language assistance. NR 73 TC 9 Z9 9 U1 2 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2014 VL 313 BP 319 EP 328 DI 10.1016/j.foreco.2013.10.032 PG 10 WC Forestry SC Forestry GA AB3BF UT WOS:000331665600035 ER PT J AU Tait, RG Shackelford, SD Wheeler, TL King, DA Casas, E Thallman, RM Smith, TPL Bennett, GL AF Tait, R. G., Jr. Shackelford, S. D. Wheeler, T. L. King, D. A. Casas, E. Thallman, R. M. Smith, T. P. L. Bennett, G. L. TI mu-Calpain, calpastatin, and growth hormone receptor genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in Angus cattle selected to increase minor haplotype and allele frequencies SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef; beef tenderness; calpain; calpastatin; carcass quality; marker association ID INFRARED REFLECTANCE SPECTROSCOPY; SINGLE NUCLEOTIDE POLYMORPHISM; BEEF-CATTLE; LONGISSIMUS TENDERNESS; GENOTYPE PROBABILITIES; ONLINE CLASSIFICATION; EFFICIENT COMPUTATION; MEAT TENDERNESS; ASSOCIATION; MARKERS AB Genetic marker effects and interactions are estimated with poor precision when minor marker allele frequencies are low. An Angus population was subjected to marker assisted selection for multiple years to increase divergent haplotype and minor marker allele frequencies to 1) estimate effect size and mode of inheritance for previously reported SNP on targeted beef carcass quality traits; 2) estimate effects of previously reported SNP on nontarget performance traits; and 3) evaluate tenderness SNP specific residual variance models compared to a single residual variance model for tenderness. Divergent haplotypes within mu-calpain (CAPN1), and SNP within calpastatin (CAST) and growth hormone receptor (GHR) were successfully selected to increase their frequencies. Traits evaluated were birth BW, weaning BW, final BW, fat thickness, LM area, USDA marbling score, yield grade, slice shear force (SSF), and visible and near infrared predicted slice shear force. Both CAPN1 and CAST exhibited additive (P < 0.001) modes of inheritance for SSF and neither exhibited dominance (P >= 0.19). Furthermore, the interaction between CAPN1 and CAST for SSF was not significant (P = 0.55). Estimated additive effects of CAPN1 (1.049 kg) and CAST (1.257 kg) on SSF were large in this study. Animals homozygous for tender alleles at both CAPN1 and CAST would have 4.61 kg lower SSF (38.6% of the mean) than animals homozygous tough for both markers. There was also an effect of CAST on yield grade (P < 0.02). The tender CAST allele was associated with more red meat yield and less trimmable fat. There were no significant effects (P >= 0.23) for GHR on any of the traits evaluated in this study. Furthermore, CAST specific residual variance models were found to fit significantly better (P < 0.001) than single residual variance models for SSF, with the tougher genotypes having larger residual variance. Thus, the risk of a tough steak from the undesired CAST genotype is increased through both an increase in mean and an increase in variation. This work confirms the importance of CAPN1 and CAST for tenderness in beef, provides a new effect of CAST on beef tenderness, and questions the utility of GHR as a selection marker for beef quality. C1 [Tait, R. G., Jr.; Shackelford, S. D.; Wheeler, T. L.; King, D. A.; Casas, E.; Thallman, R. M.; Smith, T. P. L.; Bennett, G. L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Bennett, GL (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM gary.bennett@ars.usda.gov NR 29 TC 8 Z9 8 U1 1 U2 5 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2014 VL 92 IS 2 BP 456 EP 466 DI 10.2527/jas.2013-7075 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA AA4ZY UT WOS:000331106400007 PM 24398843 ER PT J AU Mack, LA Lay, DC Eicher, SD Johnson, AK Richert, BT Pajor, EA AF Mack, L. A. Lay, D. C., Jr. Eicher, S. D. Johnson, A. K. Richert, B. T. Pajor, E. A. TI Growth and reproductive development of male piglets are more vulnerable to midgestation maternal stress than that of female piglets SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE gestation; growth; prenatal stress; reproduction; social; swine ID PRENATAL SOCIAL STRESS; REPEATED ACTH APPLICATION; OFFSPRING SEX-RATIO; GROUP-HOUSED SOWS; PREGNANT SOWS; BIRTH-WEIGHT; LITTER SIZE; PRIMIPAROUS SOWS; DOMESTIC PIGS; INTRAUTERINE POSITION AB In many mammalian species, prenatal stress masculinizes female and feminizes male offspring impairing their reproductive capacity. Regrouping gestating sows is a common, stressful production practice, but its impact on the developing pigs of the sow is not fully known. This study examined the effects of regrouping gestating sows and the administration of exogenous glucocorticoids on the growth and external reproductive morphology of pigs. At 37.2 +/- 0.26 d of gestation, 6 cohorts of 18 sows (N = 108) were placed in 1 of 3 treatments: socially stable (Stable), hydrocortisone acetate (HCA), or mixed (Mixed). The HCA sows were administered 70 mg HCA, a synthetic glucocorticoid, twice daily during the 21 d experimental period. Each Mixed sow was penned with 2 companion sows (Companion) and regrouped on d 7 and 14 with 2 different Companion sows in a new pen. Stable and HCA sows were penned in treatment groups of 3 sows. Sow social rank was assessed weekly during feeding. After the 21 d experimental period, all sows were housed in gestation stalls for the duration of pregnancy. During the 21 d, Companion sows gained more weight than HCA and Mixed sows (P < 0.05) with Stable sows intermediate. High ranked sows gained more weight than middle and low ranked sows (P < 0.05). Mixed sows had greater head lesion scores than Stable and HCA sows (P < 0.05) with Companion sows intermediate. Head lesions increased with lower social rank (P < 0.001). Sow treatment did not affect farrowing rate, litter size, or sex ratio (P > 0.10). Social rank also had no effect on farrowing rate (P > 0.10), but affected total litter size (P = 0.03). High ranked sows bore and weaned more live females than low ranked sows (P < 0.05), in part due to differential preweaning mortality among female pigs (P = 0.01). Only male pigs were affected by sow treatment. Preweaning mortality was higher among male pigs from HCA than from Mixed sows (P = 0.04) with other treatments intermediate. Despite no weight differences in the preweaning period, at 160 d of age males from HCA sows weighed more than males from Stable sows (P = 0.01) with other treatments intermediate. Males born to Companion sows had longer relative anogenital distances, a marker of fetal testosterone exposure, than males from Mixed sows (P = 0.03) with other treatments intermediate. The prenatal environment affected the pigs in a sex-specific manner altering the growth and reproductive morphology of the males more than that of the females. C1 [Mack, L. A.; Richert, B. T.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Lay, D. C., Jr.; Eicher, S. D.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Johnson, A. K.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Pajor, E. A.] Univ Calgary, Dept Prod Anim Hlth, Calgary, AB T2N IN4, Canada. RP Mack, LA (reprint author), Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. EM lauriem@vet.upenn.edu RI Johnson, Anna/E-9319-2015 OI Johnson, Anna/0000-0001-7687-0465 NR 112 TC 5 Z9 6 U1 3 U2 24 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2014 VL 92 IS 2 BP 530 EP 548 DI 10.2527/jas.2013-6773 PG 19 WC Agriculture, Dairy & Animal Science SC Agriculture GA AA4ZY UT WOS:000331106400014 PM 24398844 ER PT J AU Kim, M Kim, J Kuehn, LA Bono, JL Berry, ED Kalchayanand, N Freetly, HC Benson, AK Wells, JE AF Kim, M. Kim, J. Kuehn, L. A. Bono, J. L. Berry, E. D. Kalchayanand, N. Freetly, H. C. Benson, A. K. Wells, J. E. TI Investigation of bacterial diversity in the feces of cattle fed different diets SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE 16S ribosomal ribonucleic acid gene; diet; fecal microbiota; feedlot cattle; operational taxonomic units; pyrosequencing ID WET DISTILLERS GRAINS; RDNA CLONE LIBRARIES; 16S RIBOSOMAL-RNA; PHYLOGENETIC ANALYSIS; SP NOV.; BEEF-CATTLE; GEN. NOV.; MICROBIAL DIVERSITY; SOLUBLES; RUMEN AB The objective of this study is to investigate individual animal variation of bovine fecal microbiota including as affected by diets. Fecal samples were collected from 426 cattle fed 1 of 3 diets typically fed to feedlot cattle: 1) 143 steers fed finishing diet (83% dry-rolled corn, 13% corn silage, and 4% supplement), 2) 147 steers fed late growing diet (66% dry-rolled corn, 26% corn silage, and 8% supplement), and 3) 136 heifers fed early growing diet (70% corn silage and 30% alfalfa haylage). Bacterial 16S rRNA gene amplicons were determined from individual fecal samples using next-generation pyrosequencing technology. A total of 2,149,008 16S rRNA gene sequences from 333 cattle with at least 2,000 sequences were analyzed. Firmicutes and Bacteroidetes were dominant phyla in all fecal samples. At the genus level, Oscillibacter, Turicibacter, Roseburia, Fecalibacterium, Coprococcus, Clostridium, Prevotella, and Succinivibrio were represented by more than 1% of total sequences. However, numerous sequences could not be assigned to a known genus. Dominant unclassified groups were unclassified Ruminococcaceae and unclassified Lachnospiraceae that could be classified to a family but not to a genus. These dominant genera and unclassified groups differed (P < 0.001) with diets. A total of 176,692 operational taxonomic units (OTU) were identified in combination across all the 333 cattle. Only 2,359 OTU were shared across 3 diet groups. UniFrac analysis showed that bacterial communities in cattle feces were greatly affected by dietary differences. This study indicates that the community structure of fecal microbiota in cattle is greatly affected by diet, particularly between forage-and concentrate-based diets. C1 [Kim, M.; Kuehn, L. A.; Bono, J. L.; Berry, E. D.; Kalchayanand, N.; Freetly, H. C.; Wells, J. E.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Kim, J.; Benson, A. K.] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. RP Wells, JE (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Jim.Wells@ars.usda.gov FU USDA [2011-67005-30060] FX Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply approval to the exclusion of other products that may be suitable. The research was supported in part by USDA Project Number 2011-67005-30060. NR 44 TC 16 Z9 17 U1 9 U2 51 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2014 VL 92 IS 2 BP 683 EP 694 DI 10.2527/jas.2013-6841 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA AA4ZY UT WOS:000331106400030 PM 24352967 ER PT J AU Wang, XG Wallis, CM Daane, KM AF Wang, Xin-Geng Wallis, Christopher M. Daane, Kent M. TI Tri-trophic movement of carotenoid pigments from host plant to the parasitoid of a caterpillar SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Carotenoid; Caterpillar; Color polymorphism; Parasitoid; Pigment; Tri-trophic movement ID OBLIQUEBANDED LEAFROLLER LEPIDOPTERA; GELECHIAE HYMENOPTERA-BRACONIDAE; BROWN APPLE MOTH; COLOR POLYPHENISM; DIET; POLYMORPHISM; TORTRICIDAE; FITNESS; BIOLOGY; SEQUESTRATION AB Insect parasitoids normally produce white colored eggs. Habrobracon gelechiae (Hymenoptera: Braconidae) is a gregarious ectoparasitoid of various caterpillars. We found that adult female H. gelechiae lays yellow colored eggs when its larvae developed from host larvae of Choristoneura rosaceana and Epiphyas postvittana (both Lepidoptera: Tortricidae) that were fed green plant leaves, but white colored eggs when these same host larvae species were fed non-plant diets. This study investigated the causes of egg color in H. gelechiae and the possible consequences in terms of parasitoid fitness resulting from differential egg color. Using high-performance liquid chromatography we demonstrated that the yellow coloration resulted from the uptake of carotenoid plant pigments (mainly lutein and beta-carotene) that were initially ingested by the caterpillar larvae from plant leaves, later absorbed by the parasitoid larvae (F-0) feeding on the host and carried over to the adult parasitoids, and finally translocated to the eggs (F-1) of the parasitoids. The amount of plant pigments consumed by the parasitoid larvae (F-0) affected the intensity of the yellow color of the parasitoid's eggs (F-1). Similarly, egg color was affected by the adult female parasitoids lifetime egg production and deposition rate. Further tests suggest that the observed differences in egg color did not have a genetic basis and did not affect egg viability or fitness. To our best knowledge, this is the first report of a tri-trophic and multi-stage translocation of carotenoid plant pigments in parasitoids. We discuss possible evolutionary significance and putative functions of the absorption of plant pigments by parasitoid species. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Wang, Xin-Geng; Daane, Kent M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Wallis, Christopher M.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Wang, XG (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, 137 Mulford Hall, Berkeley, CA 94720 USA. EM xggwang@ucanr.edu FU California Pistachio Commission; California Department of Food and Agriculture; University of California IPM FX We thank Karmit Levy, Emily Kuhn, Sean Tomajian, and Mathew Middleton (University of California, Berkeley, CA) for assistance and Themis Michailides (University of California, Davis, CA) for providing several chemicals for the extraction of plant pigments. We also thank the California Pistachio Commission, the California Department of Food and Agriculture, and University of California IPM Grants Program for financial support. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal employment opportunity provider and employer. NR 45 TC 0 Z9 0 U1 4 U2 26 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD FEB PY 2014 VL 61 BP 58 EP 65 DI 10.1016/j.jinsphys.2014.01.001 PG 8 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA AB5VG UT WOS:000331856200008 PM 24424343 ER PT J AU Iverson, L Echeverria, C Nahuelhual, L Luque, S AF Iverson, Louis Echeverria, Cristian Nahuelhual, Laura Luque, Sandra TI Ecosystem services in changing landscapes: An introduction SO LANDSCAPE ECOLOGY LA English DT Editorial Material DE Landscape ecology; Ecosystem services; Provisioning; Supporting; Regulating; Cultural; South America; Planning; IUFRO-LE ID ECOLOGY; SUSTAINABILITY; CONSEQUENCES; RESTORATION; PRIORITIES; FUTURE; TRENDS AB The concept of ecosystem services from landscapes is rapidly gaining momentum as a language to communicate values and benefits to scientists and lay alike. Landscape ecology has an enormous contribution to make to this field, and one could argue, uniquely so. Tools developed or adapted for landscape ecology are being increasingly used to assist with the quantification, modelling, mapping, and valuing of ecosystem services. Several of these tools and methods encased therein are described among the eleven papers presented in this special issue, and their application has the potential to facilitate the management and promotion of services within ecosystems. Papers are associated with each of the four key categories of services that ecosystems provide to humans: supporting, provisioning, regulating, and cultural. The papers represent work conducted in eleven different countries, especially from South America. Each carries a unique approach to address a particular question pertaining to a particular set of ecosystem services. These studies are designed to inform and improve the economic, environmental and social values of the ecosystem services. This knowledge should help to develop new management alternatives for sustaining and planning ecosystems and the services they provide at different scales in space and time. We believe that these papers will create interest and inform management of some potential methods to evaluate ecosystem services at the landscape level with an integrative approach, offering new tools for management and conservation. C1 [Iverson, Louis] US Forest Serv, Delaware, OH 43015 USA. [Echeverria, Cristian] Univ Concepcion, Fac Ciencias Forest, Lab Ecol Paisaje, Concepcion, Chile. [Nahuelhual, Laura] Univ Austral Chile, Inst Econ Agr, Valdivia, Chile. [Nahuelhual, Laura] Univ Austral Chile, Ctr Climate & Resilience Res, Valdivia, Chile. [Luque, Sandra] Natl Res Inst Sci & Technol Environm & Agr, IRSTEA, Clermont Ferrand, France. [Luque, Sandra] Univ St Andrews, Dept Geog & Sustainable Dev, St Andrews, Fife, Scotland. RP Iverson, L (reprint author), US Forest Serv, 359 Main Rd, Delaware, OH 43015 USA. EM liverson@fs.fed.us RI Iverson, Louis/C-7554-2009 OI Iverson, Louis/0000-0001-9501-471X NR 33 TC 12 Z9 14 U1 10 U2 101 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD FEB PY 2014 VL 29 IS 2 BP 181 EP 186 DI 10.1007/s10980-014-9993-2 PG 6 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA AB6YE UT WOS:000331935100001 ER PT J AU Matthews, SN Iverson, LR Peters, MP Prasad, AM Subburayalu, S AF Matthews, Stephen N. Iverson, Louis R. Peters, Matthew P. Prasad, Anantha M. Subburayalu, Sakthi TI Assessing and comparing risk to climate changes among forested locations: implications for ecosystem services SO LANDSCAPE ECOLOGY LA English DT Article DE Climate change; Ecosystem services; Trees; Eastern United States; Forest composition; Prediction ID HABITAT MODELS; UNITED-STATES; FUTURE; CLASSIFICATION; CONSEQUENCES; BIODIVERSITY; UNCERTAINTY; ASSESSMENTS; DISTURBANCE; DIVERSITY AB Forests provide key ecosystem services (ES) and the extent to which the ES are realized varies spatially, with forest composition and cultural context, and in breadth, depending on the dominant tree species inhabiting an area. We address the question of how climate change may impact ES within the temperate and diverse forests of the eastern United States. We quantify the vulnerability to changes in forest habitat by 2100, based on the overall pressures of community change from an aggregation of current and potential future habitats for 134 tree species at each of 149 US Department of Defense installations. To do so, we derive an index, Forest-Related Index of Climate Vulnerability, composed of several indicators of vulnerability for each site. Further, a risk matrix (likelihood 9 consequences) provides a visual cue to compare vulnerabilities among species (example from Pennsylvania) or among sites [example for Acer saccharum (sugar maple) in Vermont vs. Kentucky]. Potential changes in specific ES can then be qualitatively examined. For example in Pennsylvania, the loss of the provisioning services (wood products) of Prunus serotina (black cherry) and Fraxinus americana (white ash) habitat projected for the future will not likely be compensated for by concomitant increases in Juniperus virginiana (redcedar) and Pinus echinata (shortleaf pine) habitat. Taken together, this approach provides a conceptual framework that allows for consideration of how potential changes in tree species habitats, as impacted by climate change, can be combined to explore relative changes in important ES that forests provide. C1 [Matthews, Stephen N.; Subburayalu, Sakthi] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. [Matthews, Stephen N.; Iverson, Louis R.; Peters, Matthew P.; Prasad, Anantha M.] US Forest Serv, No Res Stn, USDA, Delaware, OH 43015 USA. RP Matthews, SN (reprint author), Ohio State Univ, Sch Environm & Nat Resources, 2021 Coffey Rd, Columbus, OH 43210 USA. EM matthews.204@osu.edu RI Iverson, Louis/C-7554-2009 OI Iverson, Louis/0000-0001-9501-471X FU Northern Research Station; Northern Global Change Program; Northern Institute for Applied Climate Science of the US Forest Service; US Army's Environmental Quality Technology Applied and Advanced Research Program; National Science Foundation (DEB-Ecosystem Studies) [0948780] FX Thanks to the Northern Research Station, the Northern Global Change Program, and the Northern Institute for Applied Climate Science of the US Forest Service for support for this work. Further, this work was supported by the US Army's Environmental Quality Technology Applied and Advanced Research Program (Elizabeth Ferguson, Technical Director). Additional support was provided by The National Science Foundation (DEB-Ecosystem Studies 0948780). We thank Mark Ford and Eric Britzke for providing valuable comment to an earlier draft and to the reviewers and editors responsible for improving the manuscript. NR 52 TC 5 Z9 5 U1 8 U2 73 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD FEB PY 2014 VL 29 IS 2 BP 213 EP 228 DI 10.1007/s10980-013-9965-y PG 16 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA AB6YE UT WOS:000331935100004 ER PT J AU Reardon, CL Magnuson, TS Boyd, ES Leavitt, WD Reed, DW Geesey, GG AF Reardon, C. L. Magnuson, T. S. Boyd, E. S. Leavitt, W. D. Reed, D. W. Geesey, G. G. TI Hydrogenase Activity of Mineral-Associated and Suspended Populations of Desulfovibrio desulfuricans Essex 6 SO MICROBIAL ECOLOGY LA English DT Article ID SULFATE-REDUCING BACTERIA; NICKEL-IRON HYDROGENASE; VULGARIS HILDENBOROUGH; IN-SITU; PERIPLASMIC HYDROGENASE; LACTATE METABOLISM; FE HYDROGENASE; RT-PCR; REDUCTION; RNA AB The interactions between sulfate-reducing microorganisms and iron oxides influence a number of important redox-sensitive biogeochemical processes including the formation of iron sulfides. Enzymes, such as hydrogenase which catalyze the reversible oxidation of molecular hydrogen, are known to mediate electron transfer to metals and may contribute to the formation and speciation of ferrous sulfides formed at the cell-mineral interface. In the present study, we compared the whole cell hydrogenase activity of Desulfovibrio desulfuricans strain Essex 6 growing as biofilms on hematite (hematite-associated) or as suspended populations using different metabolic pathways. Hematite-associated cells exhibited significantly greater hydrogenase activity than suspended populations during sulfate respiration but not during pyruvate fermentation. The enhanced activity of the hematite-associated, sulfate-grown cells appears to be dependent on iron availability rather than a general response to surface attachment since the activity of glass-associated cells did not differ from that of suspended populations. Hydrogenase activity of pyruvate-fermenting cells was stimulated by addition of iron as soluble Fe(II)Cl-2 and, in the absence of added iron, both sulfate-reducing and pyruvate-fermenting cells displayed similar rates of hydrogenase activity. These data suggest that iron exerts a stronger influence on whole cell hydrogenase activity than either metabolic pathway or mode of growth. The location of hydrogenase to the cell envelope and the enhanced activity at the hematite surface in sulfate-reducing cells may influence the redox conditions that control the species of iron sulfides on the mineral surface. C1 [Reardon, C. L.] ARS, Columbia Plateau Conservat Res Ctr, USDA, Adams, OR 97810 USA. [Magnuson, T. S.] Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA. [Boyd, E. S.] Montana State Univ, Dept Chem & Biochem, Bozeman, MT 59717 USA. [Reed, D. W.] Biol & Chem Proc Dept, Idaho Falls, ID 83415 USA. [Leavitt, W. D.; Geesey, G. G.] Montana State Univ, Dept Microbiol, Bozeman, MT 59717 USA. [Reardon, C. L.] USDA ARS, Pendleton, OR 97801 USA. RP Reardon, CL (reprint author), USDA ARS, POB 370, Pendleton, OR 97801 USA. EM catherine.reardon@ars.usda.gov RI Reed, David/C-3337-2017 OI Reed, David/0000-0003-4877-776X FU Inland Northwest Research Alliance [MSU003] FX The authors would like to thank M. Mazzola and S. L. Strauss for the review of the manuscript and A. Lasher for technical assistance. This work was funded by Inland Northwest Research Alliance grant MSU003. NR 53 TC 0 Z9 0 U1 1 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0095-3628 EI 1432-184X J9 MICROB ECOL JI Microb. Ecol. PD FEB PY 2014 VL 67 IS 2 BP 318 EP 326 DI 10.1007/s00248-013-0308-y PG 9 WC Ecology; Marine & Freshwater Biology; Microbiology SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Microbiology GA AB3RI UT WOS:000331707500009 PM 24194097 ER PT J AU Heymsfield, SB Avena, NM Baier, L Brantley, P Bray, GA Burnett, LC Butler, MG Driscoll, DJ Egli, D Elmquist, J Forster, JL Goldstone, AP Gourash, LM Greenway, FL Han, JC Kane, JG Leibel, RL Loos, RJF Scheimann, AO Roth, CL Seeley, RJ Sheffield, V Tauber, M Vaisse, C Wang, LH Waterland, RA Wevrick, R Yanovski, JA Zinn, AR AF Heymsfield, Steven B. Avena, Nicole M. Baier, Leslie Brantley, Phillip Bray, George A. Burnett, Lisa C. Butler, Merlin G. Driscoll, Daniel J. Egli, Dieter Elmquist, Joel Forster, Janice L. Goldstone, Anthony P. Gourash, Linda M. Greenway, Frank L. Han, Joan C. Kane, James G. Leibel, Rudolph L. Loos, Ruth J. F. Scheimann, Ann O. Roth, Christian L. Seeley, Randy J. Sheffield, Val Tauber, Maithe Vaisse, Christian Wang, Liheng Waterland, Robert A. Wevrick, Rachel Yanovski, Jack A. Zinn, Andrew R. TI Hyperphagia: Current Concepts and Future Directions Proceedings of the 2nd International Conference on Hyperphagia SO OBESITY LA English DT Article ID BARDET-BIEDL-SYNDROME; PRADER-WILLI-SYNDROME; BODY-MASS INDEX; GENOME-WIDE ASSOCIATION; FOOD ADDICTION; HYPOTHALAMIC OBESITY; BARIATRIC SURGERY; ENERGY-INTAKE; PIMA-INDIANS; PARAVENTRICULAR NUCLEUS AB Objective: Hyperphagia is a central feature of inherited disorders (e.g., Prader-Willi Syndrome) in which obesity is a primary phenotypic component. Hyperphagia may also contribute to obesity as observed in the general population, thus raising the potential importance of common underlying mechanisms and treatments. Substantial gaps in understanding the molecular basis of inherited hyperphagia syndromes are present as are a lack of mechanistic of mechanistic targets that can serve as a basis for pharmacologic and behavioral treatments. Design and Methods: International conference with 28 experts, including scientists and caregivers, providing presentations, panel discussions, and debates. Results: The reviewed collective research and clinical experience provides a critical body of new and novel information on hyperphagia at levels ranging from molecular to population. Gaps in understanding and tools needed for additional research were identified. Conclusions: This report documents the full scope of important topics reviewed at a comprehensive international meeting devoted to the topic of hyperphagia and identifies key areas for future funding and research. C1 [Heymsfield, Steven B.; Brantley, Phillip; Bray, George A.; Greenway, Frank L.] Louisiana State Univ Syst, Pennington Biomed Res Ctr, Baton Rouge, LA USA. [Avena, Nicole M.] Univ Florida, Coll Med, Dept Psychiat, Gainesville, FL USA. [Baier, Leslie] NIDDK, Diabet Mol Genet Sect, Phoenix Epidemiol & Clin Res Branch, NIH, Phoenix, AZ USA. [Burnett, Lisa C.; Egli, Dieter; Leibel, Rudolph L.; Wang, Liheng] Columbia Univ, Coll Phys & Surg, New York, NY USA. [Butler, Merlin G.] Univ Kansas, Med Ctr, Kansas City, KS 66103 USA. [Driscoll, Daniel J.] Univ Florida, Coll Med, Dept Pediat, Div Genet & Metab, Gainesville, FL USA. [Egli, Dieter] New York Stem Cell Fdn, New York, NY USA. [Elmquist, Joel] UT Southwestern Med Ctr, Dallas, TX USA. [Forster, Janice L.; Gourash, Linda M.] Pittsburgh Partnership, Pittsburgh, PA USA. [Goldstone, Anthony P.] Imperial Coll London, MRC Clin Sci Ctr, Metab & Mol Imaging Grp, London, England. [Han, Joan C.; Yanovski, Jack A.] NICHHD, Sect Growth & Obes, NIH, Bethesda, MD 20892 USA. [Kane, James G.] Prader Willi Syndrome Assoc USA, Sarasota, FL USA. [Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Mindich Child Hlth & Dev Inst, Charles Bronfman Inst Personalized Med, Genet Obes & Related Metab Traits Program, New York, NY USA. [Scheimann, Ann O.] Johns Hopkins Sch Med, Div Pediat Gastroenterol Nutr & Hepatol, Baltimore, MD USA. [Roth, Christian L.] Seattle Childrens Res Inst, Ctr Integrat Brain Res, Seattle, WA USA. [Seeley, Randy J.] Univ Cincinnati, Ctr Excellence Obes & Diabet, Cincinnati, OH USA. [Sheffield, Val] Univ Iowa, Coll Med, Iowa City, IA USA. [Tauber, Maithe] Hop Enfants, Dept Endocrinol, Toulouse, France. [Tauber, Maithe] Univ Toulouse 3, F-31062 Toulouse, France. [Vaisse, Christian] Univ Calif San Francisco, Sch Med, San Francisco, CA USA. [Waterland, Robert A.] Baylor Coll Med, USDA, ARS Childrens Nutr Res Ctr, Dept Pediat & Mol & Human Genet, Houston, TX 77030 USA. [Wevrick, Rachel] Univ Alberta, Dept Med Genet, Edmonton, AB, Canada. [Zinn, Andrew R.] UT Southwestern Med Ctr, McDermott Ctr Human Growth & Dev, Dallas, TX USA. RP Heymsfield, SB (reprint author), Louisiana State Univ Syst, Pennington Biomed Res Ctr, Baton Rouge, LA USA. EM Steven.Heymsfield@pbrc.edu RI TAUBER, Maithe/K-7386-2014; OI Goldstone, Anthony/0000-0001-8179-7071; Yanovski, Jack/0000-0001-8542-1637 FU NIH [1K24 HD01361, 1K23 DK081203, R01DK53301, RL1DK081185, P01DK088761, DK079986, DK081185]; Department of Defense [W81XWH-08-1-0025]; NIH CTSA [1UL1RR029890]; Prader-Willi Syndrome Association; USPHS, Kildehoj-Santini, University of Florida Foundation [DA-03123]; NIDDK; NICHD; [1U54 RR019478] FX DJD: NIH 1K24 HD01361; NIH 1K23 DK081203; Department of Defense W81XWH-08-1-0025; 1U54 RR019478; NIH CTSA 1UL1RR029890; JE: NIH R01DK53301, NIH RL1DK081185, and NIH P01DK088761; RW: "Best Idea Grant for Hyperphagia Research" from the Prader-Willi Syndrome Association; AZ: Supported by NIH grants DK079986 and DK081185; NA: USPHS Grant DA-03123, Kildehoj-Santini, University of Florida Foundation; LB: This research was funded by the intramural program of NIDDK; JY: This research was funded by the intramural program of NICHD. NR 100 TC 8 Z9 8 U1 0 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD FEB PY 2014 VL 22 SU 1 BP S1 EP S17 DI 10.1002/oby.20646 PG 17 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA AB9JQ UT WOS:000332109300001 PM 24574081 ER PT J AU Leyte-Lugo, M Figueroa, M Gonzalez, MD Glenn, AE Gonzalez-Andrade, M Mata, R AF Leyte-Lugo, Martha Figueroa, Mario del Carmen Gonzalez, Maria Glenn, Anthony E. Gonzalez-Andrade, Martin Mata, Rachel TI Metabolites from the endophytic fungus Sporormiella minimoides isolated from Hintonia latiflora (vol 96, pg 273, 2013) SO PHYTOCHEMISTRY LA English DT Correction C1 [Leyte-Lugo, Martha; Figueroa, Mario; Mata, Rachel] Univ Nacl Autonoma Mexico, Fac Quim, Mexico City 04510, DF, Mexico. [del Carmen Gonzalez, Maria] Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico. [Glenn, Anthony E.] USDA, Toxicol & Mycotoxin Res Unit, Russell Res Ctr, Agr Res Serv, Athens, GA 30605 USA. [Gonzalez-Andrade, Martin] Secretaria Salud Mexico, Inst Nacl Med Genom, Mexico City 04510, DF, Mexico. RP Mata, R (reprint author), Univ Nacl Autonoma Mexico, Fac Quim, Mexico City 04510, DF, Mexico. EM rachel@unam.mx RI Mata, Rachel/I-4435-2014 OI Mata, Rachel/0000-0002-2861-2768 NR 1 TC 0 Z9 0 U1 1 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9422 J9 PHYTOCHEMISTRY JI Phytochemistry PD FEB PY 2014 VL 98 BP 243 EP 243 DI 10.1016/j.phytochem.2013.11.020 PG 1 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AA3UN UT WOS:000331020000029 ER PT J AU Cabrera, A Cox, L Spokas, K Hermosin, MC Cornejo, J Koskinen, WC AF Cabrera, A. Cox, L. Spokas, K. Hermosin, M. C. Cornejo, J. Koskinen, W. C. TI Influence of biochar amendments on the sorption-desorption of aminocyclopyrachlor, bentazone and pyraclostrobin pesticides to an agricultural soil SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Biochar; Fungicide; Herbicide; Organic amendment; Sorption ID DISSOLVED ORGANIC-MATTER; HERBICIDE SORPTION; BLACK CARBON; ADSORPTION; ATRAZINE; CHARCOAL AB The many advantageous properties of biochar have led to the recent interest in the use of this carbonaceous material as a soil amendment However, there are limited studies dealing with the effect of biochar on the behavior of pesticides applied to crops. The objective of this work was to determine the effect of various biochars on the sorption desorption of the herbicides aminocyclopyrachlor (6-amino-5-chloro-2-cyclopropyl-4-pyrimidinacarboxylic acid) and bentazone (3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide) and the fungicide pyraclostrobin (methyl 2-[1-(4-chlorophenyl) pyrazol-3-yloxymethil]-N-methoxycarbanilate) to a silt loam soil. Aminocyclopyrachlor and bentazone were almost completely sorbed by the soils amended with the biochars produced from wood pellets. However, lower sorption of the herbicides was observed in the soils amended with the biochar made from macadamia nut shells as compared to the unamended soil, which was attributed to the competition between dissolved organic carbon (DOC) from the biochar and the herbicides for sorption sites. Our results showed that pyraclostrobin is highly sorbed to soil, and the addition of biochars to soil did not further increase its sorption. Thus, addition of biochars to increase the retention of low mobility pesticides in soil appears to not be necessary. On the other hand, biochars with high surface areas and low DOC contents can increase the sorption of highly mobile pesticides in soil. (C) 2013 Elsevier B.V. All rights reserved. C1 [Cabrera, A.; Cox, L.; Hermosin, M. C.; Cornejo, J.] CSIC, Inst Recursos Nat & Agrobiol Sevilla IRNASE, E-41080 Seville, Spain. [Spokas, K.; Koskinen, W. C.] ARS, USDA, St Paul, MN USA. RP Cabrera, A (reprint author), POB 1052, Seville 41080, Spain. EM macabrera@irnase.csic.es RI Cabrera, Alegria/B-4753-2012; COX, LUCIA/M-4072-2015; Spokas, Kurt/F-4839-2016; OI Cabrera, Alegria/0000-0002-0421-3324; Spokas, Kurt/0000-0002-5049-5959; , LUCIA/0000-0003-2113-4780 FU Junta de Andalucia [PAIDI-AGR-264]; MICINN [AGL 2010-21421]; European Structural and Social Funds (FEDER-FSE); CSIC [i-LINK0025]; MICINN/FECYT FX The authors thank the funding projects: PAIDI-AGR-264 from Junta de Andalucia, AGL 2010-21421 from MICINN co-financed by the European Structural and Social Funds (FEDER-FSE), and i-LINK0025 from CSIC. A. Cabrera thanks the MICINN/FECYT for a postdoctoral fellowship. NR 42 TC 35 Z9 40 U1 15 U2 128 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 1 PY 2014 VL 470 BP 438 EP 443 DI 10.1016/j.scitotenv.2013.09.080 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA AA9LS UT WOS:000331415600047 PM 24144943 ER PT J AU Dubey, JP Van Why, K Verma, SK Choudhary, S Kwok, OCH Khan, A Behinke, MS Sibley, LD Ferreira, LR Oliveira, S Weaver, M Stewart, R Su, C AF Dubey, J. P. Van Why, K. Verma, S. K. Choudhary, S. Kwok, O. C. H. Khan, A. Behinke, M. S. Sibley, L. D. Ferreira, L. R. Oliveira, S. Weaver, M. Stewart, R. Su, C. TI Genotyping Toxoplasma gondii from wildlife in Pennsylvania and identification of natural recombinants virulent to mice SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Wildlife; Seroprevalence; Isolation; Genotyping; Pennsylvania; USA ID WHITE-TAILED DEER; COYOTES CANIS-LATRANS; GENETIC-CHARACTERIZATION; HIGH PREVALENCE; POPULATION-GENETICS; ATYPICAL GENOTYPES; MEPHITIS-MEPHITIS; CLONAL LINEAGES; UNITED-STATES; NORTH-AMERICA AB Recent studies indicated the predominance of Toxoplasma gondii haplogroup 12 in wildlife in the USA. However, still little is known of the genetic diversity of this parasite circulating in wildlife. In the present study, we tested coyotes (Canis latrans), red foxes (Vulpes vulpes), white-tailed deer (Odocoileus virginianus), and geese (Branta canadensis) from the state of Pennsylvania for T. gondii infection. Antibodies to T. gondii were found in 160 of 367 animals, including 92 (34.5%) of 266 coyotes, 49 (62.0%) of 79 white-tailed deer, 17 (85.0%) of 20 red fox, and two of two Canada geese tested by the modified agglutination test (cut off titer 1:25). Tissues from 105 seropositive animals were bioassayed in mice, and viable T. gondii was isolated from 29 animals, including 10 of 53 coyotes, 11 of 16 foxes, 7 of 49 deer, and one of one goose. DNA isolated from culture-derived tachyzoites of these isolates was characterized initially using multilocus PCR-RFLP markers. Nine genotypes were revealed, including ToxoDB PCR-RFLP #1(4 isolates), #2 (2 isolates), #3 (4 isolates), #4(6 isolates), #5 (4 isolates), #54 (1 isolate), #141 (1 isolate), #143 (1 isolate), and #216 (6 isolates), indicating high genetic diversity of T. gondii in wildlife in Pennsylvania. Pathogenicity of six T. gondii isolates (5 of #216 and #141) was determined in outbred Swiss Webster mice. Three of #216 and the #141 isolates were acute virulent to mice, and the other 2 #216 isolates were intermediate virulent. To determine the extent of genetic variation of these as well as a few recently reported virulent isolates from wildlife in North America, intron sequences were generated. Analysis of intron sequences and PCR-RFLP genotyping results indicated that the #216 isolates are likely derived from recombination of the clonal type I and III lineages. To determine if T. gondii virulence can be predicted by typing, we genotyped a collection of strains using PCR-RFLP markers for polymorphic genes ROP5, ROP16, ROP18 and GRA15, which are known to interact with host immune response. The results showed that there is an association of genotypes of ROP5 and ROP18 with mouse-virulence, however, additional gene(s) may also contribute to virulence in distinct T. gondii genotypes. Published by Elsevier B.V. C1 [Dubey, J. P.; Verma, S. K.; Choudhary, S.; Kwok, O. C. H.; Ferreira, L. R.; Oliveira, S.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Van Why, K.] Wildlife Serv, Anim Plant Hlth Inspect Serv, USDA, Harrisburg, PA 17106 USA. [Khan, A.; Behinke, M. S.; Sibley, L. D.] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA. [Weaver, M.; Stewart, R.] Shippensburg Univ, Coll Arts & Sci, Dept Biol, Shippensburg, PA 17257 USA. [Su, C.] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.dubey@ars.usda.gov RI Su, Chunlei/M-1892-2013; Behnke, Michael/R-8839-2016 OI Su, Chunlei/0000-0001-8392-7108; Behnke, Michael/0000-0002-4668-8109 FU NIH [AI059176, AI036629] FX Supported in part by NIH AI059176, AI036629 grants awarded to L.D. Sibley. NR 69 TC 16 Z9 19 U1 2 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD FEB PY 2014 VL 200 IS 1-2 BP 74 EP 84 DI 10.1016/j.vetpar.2013.11.001 PG 11 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AB0UJ UT WOS:000331507300010 PM 24332401 ER PT J AU Rodriguez-Vivas, RI Miller, RJ Ojeda-Chi, MM Rosado-Aguilar, JA Trinidad-Martinez, IC de Leon, AAP AF Rodriguez-Vivas, R. I. Miller, R. J. Ojeda-Chi, M. M. Rosado-Aguilar, J. A. Trinidad-Martinez, I. C. de Leon, A. A. Perez TI Acaricide and ivermectin resistance in a field population of Rhipicephalus microplus (Acari: Ixodidae) collected from red deer (Cervus elaphus) in the Mexican tropics SO VETERINARY PARASITOLOGY LA English DT Article DE Acaricide susceptibility; Ivermectin resistance; Rhipicephalus microplus; Red deer (Cervus elaphus); Mexico; Diversified livestock production ID TICK BOOPHILUS-MICROPLUS; GATED CHLORIDE CHANNEL; POTENTIAL RISK-FACTORS; CATTLE TICK; PYRETHROID RESISTANCE; AMITRAZ RESISTANCE; NEW-CALEDONIA; 1ST REPORT; ESTERASE-ACTIVITY; YUCATAN AB In the Neotropics the control of tick infestations in red deer (Cervus elaphus) is achieved primarily through the use of acaricides and macrocyclic lactones. In Mexico, resistance to one or multiple classes of acaricides has been reported in Rhipicephalus micro plus infesting cattle, but information on acaricide susceptibility in R. microplus infesting red deer is lacking. In this study we report the level of resistance to different classes of acaricides and ivermectin in R. microplus collected from red deer in the Mexican tropics. Engorged R. microplus females were collected from a red deer farm in Yucatan, Mexico. The larval packet test was used to detect resistance to the organophosphates (OPs) chlorpyrifos and coumaphos, synthetic pyrethroids (SPs) cypermethrin and permethrin, and the phenylpyrazol, fipronil. Resistance to the formamidine amitraz (Am), and ivermectin was ascertained using the larval immersion test. Data were subjected to probit analysis to determine lethal concentrations and resistance ratios to kill 50% (RR50) and 99% (RR99) of the tick population under evaluation in relation to susceptible reference strains. Additionally, allele specific polymerase chain reaction was used to detect the sodium channel F1550I mutation associated with SP resistance in R. microplus. The R. microplus population from red deer in Yucatan showed very high resistance to the two SPs evaluated (RRs > 72.2 for cypermethrin; RR for permethrin resistance was so high a dose response curve was not possible). All individual larvae tested to detect the sodium channel F1550I mutation associated with SP resistance in R. microplus were homozygous. The same tick population showed different levels of resistance to OPs (chlorpyrifos: RR50 = 1.55, RR99 = 0.63; coumaphos: RR50 = 6.8, RR99 = 5.9), fipronil (RR50 = 1.8, RR99 = 0.9), and amitraz (RR50 = 2.3, RR99 = 4.4). Resistance to ivermectin was regarded as moderate (RR50 = 7.1, RR99 = 5.0). This is the first report of R. microplus ticks collected from red deer in Mexico with different levels of resistance to four acaricide groups and ivermectin. (C) 2013 Published by Elsevier B.V. C1 [Rodriguez-Vivas, R. I.; Ojeda-Chi, M. M.; Rosado-Aguilar, J. A.; Trinidad-Martinez, I. C.] Univ Autanoma Yucatan, Fac Med Vet & Zootecnia, Cuerpo Acad Salud Anim, Merida 97100, Yucatan, Mexico. [Miller, R. J.] ARS, USDA, Cattle Fever Tick Res Lab, Edinburg, TX 78541 USA. [de Leon, A. A. Perez] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Rodriguez-Vivas, RI (reprint author), CCBA UADY, Km 15-5 Carretera Merida Xmatkuil, Merida, Yucatan, Mexico. EM rvivas@uady.mx FU FIRA-CCDT [SISTPROY: FMVZ-2012-0006]; Laboratorio Revetmex [SISTPROY: FMVZ-2012-0006]; Laboratorio Lapisa S.A. de C.V. [SISTPROY: FMVZ-2012-0006] FX We are very grateful to FIRA-CCDT, Laboratorio Revetmex and Laboratorio Lapisa S.A. de C.V. for supporting this project (SISTPROY: FMVZ-2012-0006). We thank Dave Krska and Jason Tidwell of ARS-USDA for technical assistance. This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation by the USDA for its use. The USDA is an equal opportunity provider and employer. NR 57 TC 6 Z9 6 U1 0 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD FEB PY 2014 VL 200 IS 1-2 BP 179 EP 188 DI 10.1016/j.vetpar.2013.11.025 PG 10 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AB0UJ UT WOS:000331507300024 PM 24365245 ER PT J AU Gennari, SM Ogrzewalska, M Soares, HS Saraiva, DG Pinter, A Labruna, MB Dubey, JP AF Gennari, S. M. Ogrzewalska, M. Soares, H. S. Saraiva, D. G. Pinter, A. Labruna, M. B. Dubey, J. P. TI Occurrence of Toxoplasma gondii antibodies in birds from the Atlantic Forest, state of Sao Paulo, Brazil SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Birds; Atlantic Forest; Brazil ID WILD BIRDS; CATS AB Avian are considered important intermediate hosts for Toxoplasma gondii because they serve as source of infection for Felidae, which shed environmentally resistant oocysts after ingesting infected tissues. Little is known of epidemiology of toxoplasmosis in wild birds. In the present study, antibodies to T. gondii were determined in 202 wild birds of 37 species captured in seven small areas of the Atlantic Forest, in the state of Sao Paulo, Brazil, and provided information on possible associated risk factors. Antibodies to T. gondii were assayed by the modified agglutination test (MAT) and found in 73 (36.1%) of 202 birds with titers of 1:5 in 16 samples, 1:10 in 26 samples, 1:20 in 17 samples, 1:40 in 10 samples, 1:80 in three samples, and 1:160 in one sample. No association was observed between T. gondii seropositivity and the local where the birds were collected. Seropositivity was higher in birds that lived on the forest floor (p<0.001; U = 1230.0), and in omnivorous birds (p=0.007; U=3939.0). T. gondii antibodies were reported for the first time in 23 species of birds enlarging the host range of this parasite. Notably, T. gondii antibodies were found in 83.3% (15/18) of the Rufous-bellied Thrush (Turdus rufiventris). (C) 2013 Elsevier B.V. All rights reserved. C1 [Gennari, S. M.; Ogrzewalska, M.; Soares, H. S.; Saraiva, D. G.; Labruna, M. B.] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo, Brazil. [Pinter, A.] Superintendencia Controle Endemias Sucen, Sao Paulo, Brazil. [Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Gennari, SM (reprint author), Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Av Prof Orlando Marques de Paiva,87,Cidade Univ, BR-05508270 Sao Paulo, Brazil. EM sgennari@usp.br FU State of Sao Paulo Research Foundation (FAPESP); National Council of Scientific and Technologic Development (CNPq) FX We would like to thanks State of Sao Paulo Research Foundation (FAPESP) for the grant to M.O., H.S.S and D.G.S, and the National Council of Scientific and Technologic Development (CNPq) for the grant to M.B.L. and S.M.G. NR 15 TC 4 Z9 4 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD FEB PY 2014 VL 200 IS 1-2 BP 193 EP 197 DI 10.1016/j.vetpar.2013.10.003 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AB0UJ UT WOS:000331507300026 PM 24332961 ER PT J AU Lichtenfels, JR Kharchenko, VA AF Lichtenfels, J. R. Kharchenko, V. A. TI Response to Stancampiano et al. (2013) Letter to the Editor SO VETERINARY PARASITOLOGY LA English DT Letter ID ILLUSTRATED IDENTIFICATION KEYS; STRONGYLID PARASITES; EQUUS-CABALLUS; NEMATODA; CYATHOSTOMINAE; EQUIDAE; ASINUS; HORSE C1 [Lichtenfels, J. R.] USDA ARS, Beltsville, MD 20705 USA. [Kharchenko, V. A.] Natl Acad Sci Ukraine, II Schmalhausen Inst Zool, UA-01601 Kiev, Ukraine. RP Lichtenfels, JR (reprint author), USDA ARS, Beltsville, MD 20705 USA. EM 2jrcgl@gmail.com RI Kharchenko, Vitaliy/F-2426-2010 OI Kharchenko, Vitaliy/0000-0002-3824-2078 NR 5 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD FEB PY 2014 VL 200 IS 1-2 BP 229 EP 230 DI 10.1016/j.vetpar.2013.12.006 PG 2 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AB0UJ UT WOS:000331507300034 PM 24373512 ER PT J AU Strong, AL Jiang, Q Zhang, Q Zheng, SL Boue, SM Elliott, S Burow, ME Bunnell, BA Wang, GD AF Strong, Amy L. Jiang, Quan Zhang, Qiang Zheng, Shilong Boue, Stephen M. Elliott, Steven Burow, Matthew E. Bunnell, Bruce A. Wang, Guangdi TI Design, Synthesis, and Osteogenic Activity of Daidzein Analogs on Human Mesenchymal Stem Cells SO ACS MEDICINAL CHEMISTRY LETTERS LA English DT Article DE Daidzein analogs; mesenchymal stem cells; BMSCs; osteogenesis ID TRANSCRIPTION FACTOR OSTERIX; BONE LOSS; IN-VIVO; RATS; PHYTOESTROGENS; OSTEOPOROSIS; PATHOGENESIS; GENISTEIN AB Osteoporosis is caused by an overstimulation of osteoclast activity and the destruction of the bone extracellular matrix. Without the normal architecture, osteoblast cells are unable to rebuild phenotypically normal bone. Hormone replacement therapy with estrogen has been effective in increasing osteoblast activity but also has resulted in the increased incidence of breast and uterine cancer. In this study we designed and synthesized a series of daidzein analogs to investigate their osteogenic induction potentials. Human bone marrow derived mesenchymal stem cells (MSCs) from three different donors were treated with daidzein analogs and demonstrated enhanced osteogenesis when compared to daidzein treatment. The enhanced osteogenic potential of these daidzein analogs resulted in increased osterix (Sp7), alkaline phosphatase (ALP), osteopontin (OPN), and insulin-like growth factor 1 (IGF-1), which are osteogenic transcription factors that regulate the maturation of osteogenic progenitor cells into mature osteoblast cells. C1 [Strong, Amy L.; Bunnell, Bruce A.] Tulane Univ, Sch Med, Ctr Stem Cell Res & Regenerat Med, New Orleans, LA 70112 USA. [Jiang, Quan; Zhang, Qiang; Zheng, Shilong; Wang, Guangdi] Xavier Univ, Dept Chem, New Orleans, LA 70125 USA. [Jiang, Quan; Zhang, Qiang; Zheng, Shilong; Wang, Guangdi] Xavier Univ, RCMI Canc Res Program, New Orleans, LA 70125 USA. [Boue, Stephen M.] USDA, So Reg Res Ctr, New Orleans, LA 70130 USA. [Elliott, Steven; Burow, Matthew E.] Tulane Univ, Sch Med, Dept Med, New Orleans, LA 70112 USA. RP Bunnell, BA (reprint author), Tulane Univ, Sch Med, Ctr Stem Cell Res & Regenerat Med, 1430 Tulane Ave, New Orleans, LA 70112 USA. EM bbunnell@tulane.edu; gwang@xula.edu FU NIMHD RCMI program [8G12MD007595]; Louisiana Cancer Research Consortium (LCRC); Department of Agriculture [58-6435-7-019]; Office of Naval Research [N00014-99-1-0763] FX This work was supported by the NIMHD RCMI program through Grant 8G12MD007595; the Louisiana Cancer Research Consortium (LCRC); Department of Agriculture Grant 58-6435-7-019; and Office of Naval Research Grant N00014-99-1-0763. NR 23 TC 9 Z9 9 U1 0 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1948-5875 J9 ACS MED CHEM LETT JI ACS Med. Chem. Lett. PD FEB PY 2014 VL 5 IS 2 BP 143 EP 148 DI 10.1021/ml400397k PG 6 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA AB0PC UT WOS:000331493600009 PM 24900787 ER PT J AU Johnston, DB McAloon, AJ AF Johnston, David B. McAloon, Andrew J. TI Protease increases fermentation rate and ethanol yield in dry-grind ethanol production SO BIORESOURCE TECHNOLOGY LA English DT Article DE Protease; Ethanol; Fermentation; Biofuel; Corn ID WHEAT MASH FERMENTATION; FUEL ALCOHOL PRODUCTION; COSTS AB The effects of acid protease and urea addition during the fermentation step were evaluated. The fermentations were also tested with and without the addition of urea to determine if protease altered the nitrogen requirements of the yeast. Results show that the addition of the protease had a statistically significant effect on the fermentation rate and yield. Fermentation rates and yields were improved with the addition of the protease over the corresponding controls without protease. Protease addition either with or with added urea resulted in a higher final ethanol yield than without the protease addition. Urea addition levels >1200 ppm of supplemental nitrogen inhibited ethanol production. The economic effects of the protease addition were evaluated by using process engineering and economic models developed at the Eastern Regional Research Center. The decrease in overall processing costs from protease addition was as high as $0.01/L (4 cent/gal) of denatured ethanol produced. Published by Elsevier Ltd. C1 [Johnston, David B.; McAloon, Andrew J.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Johnston, DB (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM david.johnston@ars.usda.gov NR 14 TC 11 Z9 11 U1 2 U2 18 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2014 VL 154 BP 18 EP 25 DI 10.1016/j.biortech.2013.11.043 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AB0DI UT WOS:000331461600003 PM 24378840 ER PT J AU Qureshi, N Singh, V Liu, S Ezeji, TC Saha, BC Cotta, A AF Qureshi, N. Singh, V. Liu, S. Ezeji, T. C. Saha, B. C. Cotta, A. TI Process integration for simultaneous saccharification, fermentation, and recovery (SSFR): Production of butanol from corn stover using Clostridium beijerinckii P260 SO BIORESOURCE TECHNOLOGY LA English DT Article DE Corn stover; Butanol/AB; Process integration; SSFR; Clostridium beijerinckii P260 ID ETHANOL ABE FERMENTATION; BIOBUTANOL PRODUCTION; IMMOBILIZED CELLS; WHEY PERMEATE; BED REACTOR; ACETONE; ACETOBUTYLICUM; PERVAPORATION; SOLVENTS; STRAW AB A simultaneous saccharification, fermentation, and recovery (SSFR) process was developed for the production of acetone-butanol-ethanol (AB or ABE), of which butanol is the main product, from corn stover employing Clostridium beijerinckii P260. Of the 86 g L (1) corn stover provided, over 97% of the sugars were released during hydrolysis and these were fermented completely with an ABE productivity of 0.34 g L (1) h (1) and yield of 0.39. This productivity is higher than 0.31 g L (1) h (1) when using glucose as a substrate demonstrating that AB could be produced efficiently from lignocellulosic biomass. Acetic acid that was released from the biomass during pretreatment and hydrolysis was also used by the culture to produce AB. An average rate of generation of sugars during corn stover hydrolysis was 0.98 g L (1) h (1). In this system AB was recovered using vacuum, and as a result of this (simultaneous product recovery), 100% sugars were used by the culture. Published by Elsevier Ltd. C1 [Qureshi, N.; Saha, B. C.; Cotta, A.] ARS, USDA, NCAUR, Bioenergy Res Unit, Peoria, IL 61604 USA. [Singh, V.] Univ Illinois, Dept Agr & Biol Engn, Urbana, IL 61801 USA. [Liu, S.] ARS, USDA, NCAUR, Renewable Prod Technol Unit, Peoria, IL 61604 USA. [Ezeji, T. C.] Ohio State Univ, Dept Anim Sci, Ohio State Agr Res & Dev Ctr OARDC, Wooster, OH 44691 USA. RP Qureshi, N (reprint author), ARS, USDA, NCAUR, Bioenergy Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM Nasib.Qureshi@ars.usda.gov RI Ezeji, Thaddeus/D-7171-2015 OI Ezeji, Thaddeus/0000-0002-8384-895X NR 35 TC 31 Z9 32 U1 5 U2 58 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2014 VL 154 BP 222 EP 228 DI 10.1016/j.biortech.2013.11.080 PG 7 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AB0DI UT WOS:000331461600031 PM 24398150 ER PT J AU Zeng, JJ Tong, ZH Wang, LT Zhu, JY Ingram, L AF Zeng, Jijiao Tong, Zhaohui Wang, Letian Zhu, J. Y. Ingram, Lonnie TI Isolation and structural characterization of sugarcane bagasse lignin after dilute phosphoric acid plus steam explosion pretreatment and its effect on cellulose hydrolysis SO BIORESOURCE TECHNOLOGY LA English DT Article DE Lignin structure; Soluble lignin; Nuclear magnetic resonance (NMR); Cellulose hydrolysis ID ENZYMATIC-HYDROLYSIS; LIGNOCELLULOSIC BIOMASS; ADSORPTION; BIOFUELS; POPLAR; STRAW; NMR AB The structure of lignin after dilute phosphoric acid plus steam explosion pretreatment process of sugarcane bagasse in a pilot scale and the effect of the lignin extracted by ethanol on subsequent cellulose hydrolysis were investigated. The lignin structural changes caused by pretreatment were identified using advanced nondestructive techniques such as gel permeation chromatography (GPC), quantitative C-13, and 2-D nuclear magnetic resonance (NMR). The structural analysis revealed that ethanol extractable lignin preserved basic lignin structure, but had relatively lower amount of beta-O-4 linkages, syringyl/guaiacyl units ratio (S/G), p-coumarate/ferulate ratio, and other ending structures. The results also indicated that approximately 8% of mass weight was extracted by pure ethanol. The bagasse after ethanol extraction had an approximate 22% higher glucose yield after enzyme hydrolysis compared to pretreated bagasse without extraction. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Zeng, Jijiao; Tong, Zhaohui; Wang, Letian] Univ Florida, IFAS, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Ingram, Lonnie] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32606 USA. RP Tong, ZH (reprint author), Univ Florida, IFAS, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. EM ztong@ufl.edu RI Zeng, Jijiao/F-1595-2015 OI Zeng, Jijiao/0000-0003-3858-7380 FU Biomass Research & Development Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2001-10006-3058]; US-India Consortium Grant from DOE FX We thank the support from National High Magnetic Field Laboratory and McKnight Brain Institute of the University of Florida for the NMR measurement. We also thank UF Bioethanol Pilot Plant to kindly provide the pretreated sugarcane bagasse samples. This project is supported by Biomass Research & Development Initiative Competitive Grant no. 2001-10006-3058 from the USDA National Institute of Food and Agriculture and the US-India Consortium Grant from DOE. NR 34 TC 16 Z9 16 U1 6 U2 89 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2014 VL 154 BP 274 EP 281 DI 10.1016/j.biortech.2013.12.072 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AB0DI UT WOS:000331461600038 PM 24412855 ER PT J AU Zelinka, SL AF Zelinka, Samuel L. TI Comparing the Methodologies in ASTM G198 Using Combined Hygrothermal-Corrosion Modeling SO CORROSION LA English DT Article DE ASTM G198; corrosion; hygrothermal modeling; preservative treated wood ID TREATED WOOD; FASTENERS; ENVIRONMENTS; RATES AB ASTM G198, "Standard test method for determining the relative corrosion performance of driven fasteners in contact with treated wood," was accepted by consensus and published in 2011. The method has two different exposure conditions for determining fastener corrosion performance in treated wood. The first method places the wood and embedded fasteners in a chamber at 95% relative humidity (RH), 32 degrees C, for 120 days. The second method involves a cyclic fog, with 48 h of fog followed by 72 h of dry for 120 days. The corrosion rate of embedded metals is an extremely sensitive function of the wood moisture content. While the wood moisture content is well defined, and fixed, in the constant RH test, the moisture content fluctuates and there are moisture gradients in the cyclic fog test. As a result, the fasteners in the cyclic fog test experience non-uniform and constantly changing conditions, which may be more or less challenging than the steady-state test. This paper presents the results from a combined hygrothermal/corrosion model to explore how the moisture content and temperature at the fastener interface differs between these two methods and shows how these differences in moisture content affect corrosion. C1 USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Zelinka, SL (reprint author), USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM szelinka@fs.fed.us FU Presidential Early Career Award for Scientists and Engineers (PECASE) FX The author acknowledges funding from the Presidential Early Career Award for Scientists and Engineers (PECASE). NR 21 TC 0 Z9 0 U1 0 U2 1 PU NATL ASSOC CORROSION ENG PI HOUSTON PA 1440 SOUTH CREEK DRIVE, HOUSTON, TX 77084-4906 USA SN 0010-9312 EI 1938-159X J9 CORROSION JI Corrosion PD FEB PY 2014 VL 70 IS 2 BP 206 EP 213 DI 10.5006/1034 PG 8 WC Materials Science, Multidisciplinary; Metallurgy & Metallurgical Engineering SC Materials Science; Metallurgy & Metallurgical Engineering GA AA6LX UT WOS:000331210900010 ER PT J AU Techen, N Parveen, I Pan, ZQ Khan, IA AF Techen, Natascha Parveen, Iffat Pan, Zhiqiang Khan, Ikhlas A. TI DNA barcoding of medicinal plant material for identification SO CURRENT OPINION IN BIOTECHNOLOGY LA English DT Review ID INTERNAL TRANSCRIBED SPACER; POLYMERASE-CHAIN-REACTION; TRNH INTERGENIC REGION; MOLECULAR AUTHENTICATION; SCAR MARKERS; SPECIES IDENTIFICATION; HERBAL MEDICINES; PCR-RFLP; SEQUENCE; ADULTERANTS AB Because of the increasing demand for herbal remedies and for authentication of the source material, it is vital to provide a single database containing information about authentic plant materials and their potential adulterants. The database should provide DNA barcodes for data retrieval and similarity search. In order to obtain such barcodes, several molecular methods have been applied to develop markers that aid with the authentication and identification of medicinal plant materials. In this review, we discuss the genomic regions and molecular methods selected to provide barcodes, available databases and the potential future of barcoding using next generation sequencing. C1 [Techen, Natascha; Parveen, Iffat; Khan, Ikhlas A.] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Techen, Natascha; Parveen, Iffat; Khan, Ikhlas A.] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, Pharmaceut Sci Res Inst, University, MS 38677 USA. [Pan, Zhiqiang] USDA ARS, NPURU, University, MS 38677 USA. [Khan, Ikhlas A.] King Saud Univ, Sch Pharm, Riyadh 11451, Saudi Arabia. RP Khan, IA (reprint author), Univ Mississippi, Sch Pharm, Dept Pharmacognosy, Natl Ctr Nat Prod Res, POB 1848, University, MS 38677 USA. EM ikhan@olemiss.edu FU Food and Drug Administration; USDA FX This research was funded in part by the Food and Drug Administration and USDA. We thank Jon Parcher for his revision of the manuscript and suggestions. NR 88 TC 50 Z9 52 U1 5 U2 77 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0958-1669 EI 1879-0429 J9 CURR OPIN BIOTECH JI Curr. Opin. Biotechnol. PD FEB PY 2014 VL 25 BP 103 EP 110 DI 10.1016/j.copbio.2013.09.010 PG 8 WC Biochemical Research Methods; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA AB0TU UT WOS:000331505800015 PM 24484887 ER PT J AU Hruby, A Meigs, JB O'Donnell, CJ Jacques, PF McKeown, NM AF Hruby, Adela Meigs, James B. O'Donnell, Christopher J. Jacques, Paul F. McKeown, Nicola M. TI Higher Magnesium Intake Reduces Risk of Impaired Glucose and Insulin Metabolism and Progression From Prediabetes to Diabetes in Middle-Aged Americans SO DIABETES CARE LA English DT Article ID FOOD FREQUENCY QUESTIONNAIRE; BETA-CELL FUNCTION; DOUBLE-BLIND; CARDIOVASCULAR-DISEASE; NONDIABETIC SUBJECTS; PROSPECTIVE COHORT; DIETARY MAGNESIUM; RANDOMIZED-TRIAL; RESISTANCE; SENSITIVITY AB OBJECTIVETo assess 7-year associations between magnesium intake and incident prediabetes and/or insulin resistance (IR), and progression from these states to type 2 diabetes.RESEARCH DESIGN AND METHODSIn 2,582 community-dwelling participants 26-81 years old at baseline, magnesium intake and risk of incident metabolic impairment, defined as impaired fasting glucose (FG) (5.6 to <7.0 mmol/L), impaired glucose tolerance (2-h postload glucose 7.8 to <11.1 mmol/L), IR, or hyperinsulinemia (90th percentile of homeostasis model assessment of IR or fasting insulin, respectively), was estimated among those with normal baseline status, and risk of incident diabetes was estimated among those with baseline metabolic impairment. In participants without incident diabetes, we examined magnesium intake in relation to 7-year changes in fasting and postload glucose and insulin, IR, and insulin sensitivity.RESULTSAfter adjusting for age, sex, and energy intake, compared with those with the lowest magnesium intake, those with the highest intake had 37% lower risk of incident metabolic impairment (P trend = 0.02), and in those with baseline metabolic impairment, higher intake was associated with 32% lower risk of incident diabetes (P trend = 0.05). In the combined population, the risk in those with the highest intake was 53% (P trend = 0.0004) of those with the lowest intake. Adjusting for risk factors and dietary fiber attenuated associations in the baseline normal population but did not substantially affect associations in the metabolically impaired. Higher magnesium intake tended to associate with lower follow-up FG and IR, but not fasting insulin, postload values, or insulin sensitivity.CONCLUSIONSMagnesium intake may be particularly beneficial in offsetting risk of developing diabetes among those at high risk. Magnesium's long-term associations with non-steady-state (dynamic) measures deserve further research. C1 [Hruby, Adela; Jacques, Paul F.; McKeown, Nicola M.] Tufts Univ, Jean Mayer US Dept Agr, Nutr Epidemiol Program, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Meigs, James B.] Massachusetts Gen Hosp, Dept Med, Div Gen Med, Boston, MA 02114 USA. [Meigs, James B.; O'Donnell, Christopher J.] Harvard Univ, Sch Med, Boston, MA USA. [O'Donnell, Christopher J.] NHLBI, Div Intramural Res, Framingham, MA USA. [O'Donnell, Christopher J.] NHLBI, Framingham Heart Study, Framingham, MA USA. [O'Donnell, Christopher J.] MGH, Dept Med, Div Cardiovasc, Boston, MA USA. RP McKeown, NM (reprint author), Tufts Univ, Jean Mayer US Dept Agr, Nutr Epidemiol Program, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu FU National Heart, Lung, and Blood Institute's FHS [N01-HC-25195]; U.S. Department of Agriculture [58-1950-0-014]; [K24-DK-080140] FX At the time of writing, A.H. was an American Heart Association Predoctoral Fellow. This work was also supported by the National Heart, Lung, and Blood Institute's FHS (Contract N01-HC-25195) and the U.S. Department of Agriculture (Agreement 58-1950-0-014). J.B.M. is supported by K24-DK-080140. NR 41 TC 19 Z9 19 U1 1 U2 11 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA SN 0149-5992 EI 1935-5548 J9 DIABETES CARE JI Diabetes Care PD FEB PY 2014 VL 37 IS 2 BP 419 EP 427 DI 10.2337/dc13-1397 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA AA4NM UT WOS:000331072800029 PM 24089547 ER PT J AU Wang, HQ Steyer, GD Couvillion, BR Rybczyk, JM Beck, HJ Sleavin, WJ Meselhe, EA Allison, MA Boustany, RG Fischenich, CJ Rivera-Monroy, VH AF Wang, Hongqing Steyer, Gregory D. Couvillion, Brady R. Rybczyk, John M. Beck, Holly J. Sleavin, William J. Meselhe, Ehab A. Allison, Mead A. Boustany, Ronald G. Fischenich, Craig J. Rivera-Monroy, Victor H. TI Forecasting landscape effects of Mississippi River diversions on elevation and accretion in Louisiana deltaic wetlands under future environmental uncertainty scenarios SO ESTUARINE COASTAL AND SHELF SCIENCE LA English DT Article DE sediment diversion; surface elevation; vertical accretion; deltaic wetlands; sea-level rise; subsidence ID SEA-LEVEL RISE; COASTAL SALT MARSHES; VERTICAL ACCRETION; SEDIMENT TRANSPORT; SURFACE ELEVATION; SOIL FORMATION; LAND LOSS; RESTORATION; WATER; ESTUARY AB Large sediment diversions are proposed and expected to build new wetlands to alleviate the extensive wetland loss (5000 km(2)) affecting coastal Louisiana during the last 78 years. Current assessment and prediction of the impacts of sediment diversions have focused on the capture and dispersal of both water and sediment on the adjacent river side and the immediate outfall marsh area. However, little is known about the effects of sediment diversions on existing wetland surface elevation and vertical accretion dynamics in the receiving basin at the landscape scale. In this study, we used a spatial wetland surface elevation model developed in support of Louisiana's 2012 Coastal Master Plan to examine such landscape-scale effects of sediment diversions. Multiple sediment diversion projects were incorporated in the model to simulate surface elevation and vertical accretion for the next 50 years (2010-2060) under two environmental (moderate and less optimistic) scenarios. Specifically, we examined landscape-scale surface elevation and vertical accretion trends under diversions with different geographical locations, diverted discharge rates, and geomorphic characteristics of the receiving basin. Model results indicate that small diversions (<283 m(3) s(-1)) tend to have limited effects of reducing landscape-scale elevation loss (<3%) compared to a future without action (FWOA) condition. Large sediment diversions (>1500 m(3) s(-1)) are required to achieve landscape-level benefits to promote surface elevation via vertical accretion to keep pace with rising sea level. Published by Elsevier Ltd. C1 [Wang, Hongqing; Steyer, Gregory D.; Couvillion, Brady R.; Beck, Holly J.; Sleavin, William J.] US Geol Survey, Natl Wetlands Res Ctr, Baton Rouge, LA 70803 USA. [Rybczyk, John M.] Western Washington Univ, Dept Environm Sci, Bellingham, WA 98225 USA. [Meselhe, Ehab A.; Allison, Mead A.] Water Inst Guf, Baton Rouge, LA 70825 USA. [Allison, Mead A.] Tulane Univ, Dept Earth & Environm Sci, New Orleans, LA 70118 USA. [Boustany, Ronald G.] Nat Resources Conservat Serv, USDA, Lafayette, LA 70506 USA. [Fischenich, Craig J.] US Army Corps Engineers, Engineer Res & Dev Ctr, Vicksburg, MS 39180 USA. [Rivera-Monroy, Victor H.] Louisiana State Univ, Dept Oceanog & Coastal Sci, Sch Coast & Environm, Baton Rouge, LA 70803 USA. RP Wang, HQ (reprint author), US Geol Survey, Coastal Restorat Assessment Branch, Natl Wetlands Res Ctr, Livestock Show Off,LSU, Baton Rouge, LA 70803 USA. EM wangh@usgs.gov RI Allison, Mead/A-7208-2010; OI Wang, Hongqing/0000-0002-2977-7732 FU State of Louisiana's Coastal Protection and Restoration Authority (LaCPRA); NSF-Coupled Natural and Human Systems program [DBCS 1212112] FX This research was supported by funds from the State of Louisiana's Coastal Protection and Restoration Authority (LaCPRA) in support of Louisiana's 2012 Coastal Master Plan. We would like to thank Nadine Trahan, Yvonne Allen, Guerry Holm Jr., and Brian Perez for their assistance in model simulations and data analysis. We thank John Andrew Nyman as well as two anonymous reviewers for their constructive review for improving this manuscript. VHRM participation was partially supported by the NSF-Coupled Natural and Human Systems program under grant No. DBCS 1212112. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 50 TC 6 Z9 6 U1 5 U2 68 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0272-7714 EI 1096-0015 J9 ESTUAR COAST SHELF S JI Estuar. Coast. Shelf Sci. PD FEB 1 PY 2014 VL 138 BP 57 EP 68 DI 10.1016/j.ecss.2013.12.020 PG 12 WC Marine & Freshwater Biology; Oceanography SC Marine & Freshwater Biology; Oceanography GA AB0VW UT WOS:000331511200006 ER PT J AU Velderrain-Rodriguez, GR Palafox-Carlos, H Wall-Medrano, A Ayala-Zavala, JF Chen, CYO Robles-Sanchez, M Astiazaran-Garcia, H Alvarez-Parrilla, E Gonzalez-Aguilar, GA AF Velderrain-Rodriguez, G. R. Palafox-Carlos, H. Wall-Medrano, A. Ayala-Zavala, J. F. Chen, C-Y. O. Robles-Sanchez, M. Astiazaran-Garcia, H. Alvarez-Parrilla, E. Gonzalez-Aguilar, G. A. TI Phenolic compounds: their journey after intake SO FOOD & FUNCTION LA English DT Review ID CATECHOL-O-METHYLTRANSFERASE; IN-VITRO; DIETARY POLYPHENOLS; GREEN TEA; ANTIOXIDANT ACTIVITY; TISSUE DISTRIBUTION; HUMAN PLASMA; PHARMACOKINETIC PROPERTIES; FOOD MICROSTRUCTURE; BIOLOGICAL-ACTIVITY AB Plant foods are rich in phenolic compounds (PCs) that display multifaceted bioactions in health promotion and disease prevention. To exert their bioactivity, they must be delivered to and absorbed in the gastrointestinal (GI) tract, transported in circulation, and reach the target tissues. During the journey from ingestion to target tissues and final excretion, PCs are subjected to modifications by many factors during their absorption, deposition, metabolism and excretion (ADME) and consequently their bioefficacy may be modified. Consistent with all nutrients in foods, PCs must first be released from the food matrix through mechanical, chemical, and enzymatic forces to facilitate absorption along the GI tract, particularly in the upper small intestine section. Further, glycosylation of PCs directs the route of their absorption with glycones being transported through active transportation and aglycones through passive diffusion. After enteral absorption, the majority of PCs are extensively transformed by the detoxification system in enterocytes and liver for excretion in bile, feces, and urine. The journey of PCs from consumption to excretion appears to be comparable to many synthetic medications, but with some dissimilarities in their fate and bioactivity after phase I and II metabolism. The overall bioavailability of PCs is determined mainly by chemical characteristics, bioaccessibility, and ADME. In this review, factors accounting for variation in PCs bioavailability are discussed because this information is crucial for validation of the health benefits of PCs and their mechanism of action. C1 [Velderrain-Rodriguez, G. R.; Palafox-Carlos, H.; Ayala-Zavala, J. F.; Astiazaran-Garcia, H.; Gonzalez-Aguilar, G. A.] Res Ctr Food & Dev CIAD, AC, Hermosillo 83000, Sonora, Mexico. [Wall-Medrano, A.; Alvarez-Parrilla, E.] Autonomous Univ Ciudad Juarez, Inst Biomed Sci, Ciudad Juarez 32310, Chihuahua, Mexico. [Chen, C-Y. O.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Robles-Sanchez, M.] Univ Sonora, Dept Invest & Posgrad Alimentos, Hermosillo 83000, Sonora, Mexico. RP Velderrain-Rodriguez, GR (reprint author), Res Ctr Food & Dev CIAD, AC, Carretera Victoria Km 0-6, Hermosillo 83000, Sonora, Mexico. EM gustavo@ciad.mx FU CIAD; CONACYT-Mexico; project "Nutrigenomica e interacciones moleculares de fenoles y fibra dietaria del mango "Ataulfo" [179574CB-2012-01] FX We thank CIAD and CONACYT-Mexico for financial support. This work is part of the project "Nutrigenomica e interacciones moleculares de fenoles y fibra dietaria del mango "Ataulfo" (Mangifera indica, L.) en un sistema Murino" Project 179574CB-2012-01. NR 115 TC 28 Z9 28 U1 7 U2 109 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND SN 2042-6496 EI 2042-650X J9 FOOD FUNCT JI Food Funct. PD FEB PY 2014 VL 5 IS 2 BP 189 EP 197 DI 10.1039/c3fo60361j PG 9 WC Biochemistry & Molecular Biology; Food Science & Technology SC Biochemistry & Molecular Biology; Food Science & Technology GA AA9SE UT WOS:000331432400001 PM 24336740 ER PT J AU Yeo, IY Lang, M Vermote, E AF Yeo, In-Young Lang, Megan Vermote, Eric TI Improved Understanding of Suspended Sediment Transport Process Using Multi-Temporal Landsat Data: A Case Study From the Old Woman Creek Estuary (Ohio) SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Landsat time-series images; remote sensing; suspended sediment; turbidity; wetland ID WATER-QUALITY; CHESAPEAKE BAY; RIVER ESTUARY; IN-SITU; REFLECTANCE; ALGORITHM; QUANTIFICATION; SPECTRUM; IMAGERY; MODELS AB We used historical water quality data, continuous in situ water quality monitoring data, and multi-temporal Landsat-7 ETM+ imagery for the period of September 1999-April 2003 to study the distribution of total suspended sediments (TSS) in Old Woman Creek (OWC), a freshwater coastal wetland adjacent to Lake Erie. A multiple linear regression model was developed to describe the relationship between turbidity and atmospherically corrected reflectance from Landsat-7 ETM+ bands 2 and 4 (R-2 = 0.65). Turbidity was then converted to total suspended sediments (TSS), based on in situ historical data. Mapped spatial patterns of TSS provided useful information on key physical drivers affecting the transport process of suspended sediment. This study demonstrates the potential and limitations of using medium-spatial scale multispectral data, such as Landsat, to understand important factors that control suspended sediment transport processes within an estuary. C1 [Yeo, In-Young] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Lang, Megan] US Forest Serv, USDA, No Res Stn, Beltsville, MD 20705 USA. [Vermote, Eric] NASA, Goddard Space Flight Ctr, Terr Informat Syst Lab, Greenbelt, MD 20771 USA. RP Yeo, IY (reprint author), Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. FU NASA Land Cover Land Use Change Program [NNX12AG21G] FX This work was supported by NASA Land Cover Land Use Change Program (contract no. NNX12AG21G). NR 50 TC 3 Z9 3 U1 3 U2 25 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD FEB PY 2014 VL 7 IS 2 BP 636 EP 647 DI 10.1109/JSTARS.2013.2265191 PG 12 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA AB0BS UT WOS:000331457400022 ER PT J AU Fabrick, JA Pei, J Hull, JJ Yool, AJ AF Fabrick, Jeffrey A. Pei, Jinxin Hull, J. Joe Yool, Andrea J. TI Molecular and functional characterization of multiple aquaporin water channel proteins from the western tarnished plant bug, Lygus hesperus SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Aquaporin; Major intrinsic protein family; Lygus hesperus; Western tarnished plant bug; Hemiptera; Superaquaporin; S-aquaporin ID TRANSMEMBRANE TOPOLOGY; ENDOPLASMIC-RETICULUM; HOMOPTERAN INSECTS; FILTER CHAMBER; BOMBYX-MORI; ION-CHANNEL; DOG TICK; MEMBRANE; IDENTIFICATION; EXPRESSION AB Aquaporins (AQPs) are integral membrane channel proteins that, facilitate the bidirectional transfer of water or other small solutes across biological membranes involved in numerous essential physiological processes. In arthropods, AQPs belong to several subfamilies, which contribute to osmoregulation, respiration, cryoprotection, anhydrobiosis, and excretion. We cloned and characterized five novel AQPs from the western tarnished plant bug, Lygus hesperus, a polyphagous insect pest of food and fiber crops throughout western North America. The L. hesperus AQPs (LhAQP1-5) belong to different phylogenetic subfamilies, have unique transcription profiles and cellular localizations, and all transport water (but not glycerol) when heterologously expressed in Xenopus laevis oocytes. Our results demonstrate that multiple AQPs with possible compensatory functions are produced in L. hesperus that likely play important roles in maintaining water homeostasis in this important insect pest. Published by Elsevier Ltd. C1 [Fabrick, Jeffrey A.; Hull, J. Joe] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Pei, Jinxin; Yool, Andrea J.] Univ Adelaide, Sch Med Sci, Adelaide, SA 5005, Australia. RP Fabrick, JA (reprint author), USDA ARS, US Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM jeff.fabrick@ars.usda.edu FU Cotton Inc. FX We thank Lolita Mathew, Lynn Forlow-Jech, Sarah Bjorklund, and Eric Hoffmann for technical support, O.P. Perera for providing partial Lygus lineolaris aquaporin transcriptome sequences (unpublished) that helped with initial cloning of LhAQPs, and Dale Spurgeon for helpful comments. This is a cooperative investigation between USDA-ARS and the University of Adelaide. The research described in this manuscript was partially supported by funds from Cotton Inc. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 97 TC 13 Z9 13 U1 5 U2 30 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 EI 1879-0240 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD FEB PY 2014 VL 45 BP 125 EP 140 DI 10.1016/j.ibmb.2013.12.002 PG 16 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA AB5VN UT WOS:000331856900012 PM 24333473 ER PT J AU Wang, H Troy, LM Rogers, GT Fox, CS McKeown, NM Meigs, JB Jacques, PF AF Wang, H. Troy, L. M. Rogers, G. T. Fox, C. S. McKeown, N. M. Meigs, J. B. Jacques, P. F. TI Longitudinal association between dairy consumption and changes of body weight and waist circumference: the Framingham Heart Study SO INTERNATIONAL JOURNAL OF OBESITY LA English DT Article DE dairy; weight; waist circumference; longitudinal; milk; yogurt ID FOOD FREQUENCY QUESTIONNAIRE; INSULIN-RESISTANCE; CARDIOVASCULAR RISK; METABOLIC SYNDROME; PROSPECTIVE COHORT; CLINICAL-TRIALS; OBESITY; ADULTS; DIET; MANAGEMENT AB BACKGROUND: Dairy foods are nutrient dense and may be protective against long-term weight gain. OBJECTIVE: We aimed to examine the longitudinal association between dairy consumption and annualized changes in weight and waist circumference (WC) in adults. METHODS: Members of the Framingham Heart Study Offspring Cohort who participated in the fifth through eighth study examinations (1991-2008) were included in these analyses (3440 participants with 11 683 observations). At each exam, dietary intake was assessed by a validated food frequency questionnaire, and weight and WC were assessed following standardized procedures. Repeated measures models were used for the longitudinal analyses of annualized weight and waist circumference changes, adjusting for time-varying or invariant covariates. RESULTS: On average, participants gained weight and WC during follow-up. Dairy intake increased across exams. After adjusting for demographic and lifestyle factors (including diet quality), participants who consumed >= 3 servings per day of total dairy had 0.10 kg (+/- 0.04) smaller annualized increment of weight (P-trend = 0.04) than those consuming <1 serving per day. Higher total dairy intake was also marginally associated with less WC gain (P-trend = 0.05). Similarly, participants who consumed >= 3 servings per week of yogurt had a 0.10 kg (+/- 0.04) and 0.13 cm (+/- 0.05) smaller annualized increment of weight (P-trend = 0.03) and WC (P-trend = 0.008) than those consuming <1 serving per week, respectively. Skim/ low-fat milk, cheese, total high-fat or total low-fat dairy intake were not associated with long-term change in weight or WC. CONCLUSION: Further longitudinal and interventional studies are warranted to confirm the beneficial role of increasing total dairy and yogurt intake, as part of a healthy and calorie-balanced dietary pattern, in the long-term prevention of gain in weight and WC. C1 [Wang, H.; Troy, L. M.; Rogers, G. T.; McKeown, N. M.; Jacques, P. F.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Program, Boston, MA 02111 USA. [Troy, L. M.] Univ Massachusetts, Dept Nutr, Chenoweth Lab, Amherst, MA 01003 USA. [Troy, L. M.; McKeown, N. M.; Jacques, P. F.] Tufts Univ, Gerald J & Dorothy R Friedman Sch Nutr Sci & Poli, Boston, MA 02111 USA. [Fox, C. S.] Framingham Heart Dis Epidemiol Study, Natl Heart Lung & Blood Inst, Framingham, MA USA. [Meigs, J. B.] Massachusetts Gen Hosp, Dept Med, Gen Internal Med Unit, Boston, MA 02114 USA. RP Jacques, PF (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM Paul.jacques@tufts.edu FU National Heart, Lung and Blood Institute of the National Institute of Health [NO1-HC-25195]; US Department of Agriculture [58-1950-7-707]; Dannon Company, Inc.; General Mills Bell Institute of Health and Nutrition FX We thank Kara A Livingston, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, for the help with data set management. This work was supported by the National Heart, Lung and Blood Institute of the National Institute of Health (contract number: NO1-HC-25195), US Department of Agriculture Agreement 58-1950-7-707 and research grants from The Dannon Company, Inc., and General Mills Bell Institute of Health and Nutrition. NR 53 TC 33 Z9 34 U1 4 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0307-0565 EI 1476-5497 J9 INT J OBESITY JI Int. J. Obes. PD FEB PY 2014 VL 38 IS 2 BP 299 EP 305 DI 10.1038/ijo.2013.78 PG 7 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA AA9HL UT WOS:000331404300020 PM 23736371 ER PT J AU Othman, Y Steele, C VanLeeuwen, D Heerema, R Bawazir, S St Hilaire, R AF Othman, Yahia Steele, Caiti VanLeeuwen, Dawn Heerema, Richard Bawazir, Salim St Hilaire, Rolston TI Remote sensing used to detect moisture status of pecan orchards grown in a desert environment SO INTERNATIONAL JOURNAL OF REMOTE SENSING LA English DT Article ID CROP WATER-STRESS; SPECTRAL INDEXES; GAS-EXCHANGE; DROUGHT; TREES; LEAF; CHLOROPHYLL; IRRIGATION; EVAPOTRANSPIRATION; IMAGERY C1 [Othman, Yahia; VanLeeuwen, Dawn; Heerema, Richard; Bawazir, Salim; St Hilaire, Rolston] New Mexico State Univ, Las Cruces, NM 88003 USA. [Steele, Caiti] New Mexico State Univ, USDA, ARS, Las Cruces, NM 88003 USA. RP St Hilaire, R (reprint author), New Mexico State Univ, Las Cruces, NM 88003 USA. EM rsthilai@nmsu.edu NR 44 TC 3 Z9 3 U1 4 U2 16 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0143-1161 EI 1366-5901 J9 INT J REMOTE SENS JI Int. J. Remote Sens. PD FEB 1 PY 2014 VL 35 IS 3 BP 949 EP 966 DI 10.1080/01431161.2013.873834 PG 18 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA AA8GJ UT WOS:000331333300008 ER PT J AU Biswas, T Ramsey, RD Bissonette, JA Symanzik, J AF Biswas, Tanushree Ramsey, R. Douglas Bissonette, John A. Symanzik, Juergen TI Integration of two spectral indices to monitor loss of moist grasslands within the Jaldapara Wildlife Sanctuary, India SO INTERNATIONAL JOURNAL OF REMOTE SENSING LA English DT Article ID LANDSAT TM IMAGERY; NATIONAL-PARK; COVER CHANGE; WEST-BENGAL; MANAGEMENT; VEGETATION; CLASSIFICATION; FRAGMENTATION; CONSERVATION; LANDSCAPES C1 [Biswas, Tanushree] US Forest Serv, Dept Agr, Salt Lake City, UT 84119 USA. [Ramsey, R. Douglas] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Bissonette, John A.] Utah State Univ, Dept Wildland Resources, Coll Nat Resources, US Geol Survey Utah Cooperat Fish & Wildlife Res, Logan, UT 84322 USA. [Symanzik, Juergen] Utah State Univ, Dept Math & Stat, Logan, UT 84322 USA. RP Biswas, T (reprint author), US Forest Serv, Dept Agr, Salt Lake City, UT 84119 USA. EM tbiswas@fs.fed.us RI Ramsey, R. Douglas/D-3504-2009 FU Ford Foundation International Fellowship Program; National Geographic Research Grant from Ashoka Trust for Research in Ecology and Environment (ATREE, Bangalore); Ecology Center, Utah State University FX We are thankful to West Bengal Forest Department, India, for giving us the permission and logistic support to work within the sanctuary. We are thankful to the Ford Foundation International Fellowship Program for funding the majority of this work, the National Geographic Research Grant from Ashoka Trust for Research in Ecology and Environment (ATREE, Bangalore) and the Ecology Center, Utah State University for funding the field work, and the Intermountain Region Digital Image Archive Center - NASA for providing satellite imagery and logistical support. NR 70 TC 0 Z9 0 U1 0 U2 10 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0143-1161 EI 1366-5901 J9 INT J REMOTE SENS JI Int. J. Remote Sens. PD FEB 1 PY 2014 VL 35 IS 3 BP 1038 EP 1063 DI 10.1080/01431161.2013.875631 PG 26 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA AA8GJ UT WOS:000331333300013 ER PT J AU Huang, SK Csaki, T Doublet, V Dussaubat, C Evans, JD Gajda, AM Gregorc, A Hamilton, MC Kamler, M Lecocq, A Muz, MN Neumann, P Ozkirim, A Schiesser, A Sohr, AR Tanner, G Tozkar, CO Williams, GR Wu, L Zheng, HQ Chen, YP AF Huang, Shao Kang Csaki, Tamas Doublet, Vincent Dussaubat, Claudia Evans, Jay D. Gajda, Anna M. Gregorc, Alex Hamilton, Michele C. Kamler, Martin Lecocq, Antoine Muz, Mustafa N. Neumann, Peter Ozkirim, Asli Schiesser, Aygun Sohr, Alex R. Tanner, Gina Tozkar, Cansu Ozge Williams, Geoffrey R. Wu, Lyman Zheng, Huoqing Chen, Yan Ping TI Evaluation of Cage Designs and Feeding Regimes for Honey Bee (Hymenoptera: Apidae) Laboratory Experiments SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Apis mellifera; cage; laboratory testing; COLOSS; honey bee ID COLONY COLLAPSE DISORDER; APIS-MELLIFERA; LONGEVITY; PROTEINS; WORKERS; DECLINE; SIZE; DIET AB The aim of this study was to improve cage systems for maintaining adult honey bee (Apis mellifera L.) workers under in vitro laboratory conditions. To achieve this goal, we experimentally evaluated the impact of different cages, developed by scientists of the international research network COLOSS (Prevention of honey bee COlony LOSSes), on the physiology and survival of honey bees. We identified three cages that promoted good survival of honey bees. The bees from cages that exhibited greater survival had relatively lower titers of deformed wing virus, suggesting that deformed wing virus is a significant marker reflecting stress level and health status of the host. We also determined that a leak- and drip-proof feeder was an integral part of a cage system and a feeder modified from a 20-ml plastic syringe displayed the best result in providing steady food supply to bees. Finally, we also demonstrated that the addition of protein to the bees' diet could significantly increase the level of vitellogenin gene expression and improve bees' survival. This international collaborative study represents a critical step toward improvement of cage designs and feeding regimes for honey bee laboratory experiments. C1 [Huang, Shao Kang] Fujian Agr & Forestry Univ, Coll Bee Sci, Fuzhou 350002, Fujian, Peoples R China. [Csaki, Tamas] Szent Istvan Univ, Inst Wildlife Conservat, Godollo, Hungary. [Doublet, Vincent] Univ Halle Wittenberg, Inst Biol, D-06099 Halle, Saale, Germany. [Dussaubat, Claudia] INRA, Lab Biol & Protect Lapos Abeille, UMR Abeilles & Environm, F-84914 Avignon, France. [Evans, Jay D.; Hamilton, Michele C.; Chen, Yan Ping] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Gajda, Anna M.] Warsaw Univ Life Sci, Fac Vet Med, PL-02786 Warsaw, Poland. [Gregorc, Alex] Agr Inst Slovenia, Ljubljana 1001, Slovenia. [Kamler, Martin] Bee Res Inst Dol, Libcice Nad Vltavou 25266, Czech Republic. [Lecocq, Antoine] Univ Copenhagen, Dept Plant & Environm Sci, DK-1871 Frederiksberg C, Denmark. [Muz, Mustafa N.] Univ Mustafa Kemal, Fac Vet Med, Dept Parasitol, TR-3100 Antakya, Hatay, Turkey. [Neumann, Peter; Tanner, Gina; Williams, Geoffrey R.] Swiss Bee Res Ctr, Agroscope Liebefeld Posieux Res Stn ALP HARAS, Bern, Switzerland. [Neumann, Peter; Tanner, Gina; Williams, Geoffrey R.] Univ Bern, Vetsuisse Fac, Inst Bee Hlth, Bern, Switzerland. [Ozkirim, Asli; Schiesser, Aygun] Hacettepe Univ, Dept Biol, Bee Hlth Lab, TR-06800 Beytepe, Ankara, Turkey. [Ozkirim, Asli; Schiesser, Aygun] Hacettepe Univ, Bee & Bee Prod Res & Applicat Ctr, TR-06800 Beytepe, Ankara, Turkey. [Sohr, Alex R.; Wu, Lyman] Univ Maryland, Dept Biol, College Pk, MD 20742 USA. [Tozkar, Cansu Ozge] Middle E Tech Univ, Dept Biol, TR-06800 Ankara, Turkey. [Zheng, Huoqing] Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China. RP Chen, YP (reprint author), USDA ARS, Bee Res Lab, BARC East Bldg 306,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM judy.chen@ars.usda.gov RI Evans, Jay/C-8408-2012; SCHIESSER, AYGUN/G-9213-2013; Williams, Geoffrey/C-9637-2015; Doublet, Vincent/D-8050-2015 OI Evans, Jay/0000-0002-0036-4651; Williams, Geoffrey/0000-0002-0093-1126; Doublet, Vincent/0000-0002-6313-1222 FU Teachers' Development Scholarship; Fujian Agriculture and Forestry University, PR China; U.S. Department of Agriculture-Cooperative Agricultural Project (USDA-CAP) grant [2009-85118-05718]; COST (European Cooperation in Science and Technology); Ricola Foundation-Nature and Culture FX We wish to express our sincere gratitude to Barton Smith, Jr., and Andy Ulsamer for providing excellent technical assistance. This work was funded by 2012 Teachers' Development Scholarship, Fujian Agriculture and Forestry University, PR China, and U.S. Department of Agriculture-Cooperative Agricultural Project (USDA-CAP) grant (2009-85118-05718). The honey bee research network COLOSS provided an opportunity for project conception and development. COLOSS was supported by COST (European Cooperation in Science and Technology) and still is supported by the Ricola Foundation-Nature and Culture. NR 34 TC 3 Z9 3 U1 1 U2 42 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 54 EP 62 DI 10.1603/EC13213 PG 9 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400006 PM 24665684 ER PT J AU Cooper, WR Nicholson, SJ Puterka, GJ AF Cooper, W. Rodney Nicholson, Scott J. Puterka, Gary J. TI Potential Transmission of Pantoea spp. and Serratia marcescens (Enterobacteriales: Enterobacteriaceae) to Plants by Lygus hesperus (Hemiptera: Miridae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE tarnished plant bug; Pantoea ananatis; vector; Serratia marcescens; horizontal transmission ID FRANKLINIELLA-FUSCA; TOBACCO THRIPS; ERWINIA-ANANAS; CAUSAL AGENT; BACTERIUM; DISEASE; ROT; ASSOCIATION; PATHOGEN; COTTON AB Lygus hesperus Knight (Hemiptera: Miridae) is a key agricultural pest in the western United States. In a recent study, proteins from Pantoea ananatis and Serratia marcescens (Enterobacteriales: Enterobacteriaceae) were identified in diet that was stylet probed and fed on by L. hesperus adults. P. ananatis and S. marcescens are ubiquitous bacteria that infect a wide range of crops. The objective of our study was to determine whether L. hesperus transfer P. ananatis and S. marcescens to food substrates during stylet-probing activities. Sucrose (5%) was spread under parafilm and exposed to adult L. hesperus for 24 h. Diet similarly prepared but not exposed to insects was used for controls. MacConkey agar was inoculated with stylet-probed or control diets and incubated at 25 degrees C. After 24 h, bacterial colonies were observed on agar that was inoculated with stylet-probed diet, but were not observed on agar inoculated with control diet. Isolated bacterial colonies were putatively identified as either Pantoea spp. or S. marcescens using the API 20e identification kit. These results indicate that L. hesperus is capable of vectoring P. ananatis and S. marcescens. C1 [Cooper, W. Rodney] USDA ARS, Western Integrated Cropping Syst Res Uni, Shafter Cotton Res Stn, Shafter, CA 93263 USA. [Puterka, Gary J.] USDA ARS, Plant Sci Res Lab, Wheat Peanuts & Other Field Crops Res Unit, Stillwater, OK 74075 USA. RP Cooper, WR (reprint author), USDA ARS, Western Integrated Cropping Syst Res Uni, Shafter Cotton Res Stn, 17053 North Shafter Ave, Shafter, CA 93263 USA. EM rodney.cooper@ars.usda.gov RI Cooper, William/D-3205-2017 NR 24 TC 1 Z9 2 U1 3 U2 22 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 63 EP 65 DI 10.1603/EC13056 PG 3 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400007 PM 24665685 ER PT J AU Krugner, R Backus, EA AF Krugner, Rodrigo Backus, Elaine A. TI Plant Water Stress Effects on Stylet Probing Behaviors of Homalodisca vitripennis (Hemiptera: Cicadellidae) Associated With Acquisition and Inoculation of the Bacterium Xylella fastidiosa SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE glassy-winged sharpshooter; deficit irrigation; Pierce's disease; electrical penetration graph; EPG ID REGULATED DEFICIT IRRIGATION; GLASSY-WINGED SHARPSHOOTER; ALMOND LEAF SCORCH; GRAPH WAVE-FORM; PIERCES-DISEASE; COAGULATA HEMIPTERA; CAUSAL AGENT; TRANSMISSION; PENETRATION; LEAFHOPPERS AB The glassy-winged sharpshooter, Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), is a xylem fluid-ingesting leafhopper that transmits Xylella fastidiosa Wells et al., a plant-infecting bacterium that causes several plant diseases in the Americas. Although the role of plant water stress on the population density and dispersal of H. vitripennis has been studied, nothing is known about the effects of plant water stress on the transmission of X. fastidiosa by H. vitripennis. A laboratory study was conducted to determine the influence of plant water stress on the sharpshooter stylet probing behaviors associated with the acquisition and inoculation of X. fastidiosa. Electrical penetration graph was used to monitor H. vitripennis feeding behaviors for 20-h periods on citrus [Citrus sinensis (L.) Osbeck] and almond [Prunus dulcis (Miller) D.A. Webb] plants subjected to levels of water stress. Adult H. vitripennis successfully located xylem vessels, then performed behaviors related to the evaluation of the xylem cell and fluid, and finally ingested xylem fluid from citrus and almond plants under the tested fluid tensions ranging from -5.5 to -33.0 bars and -6.0 to -24.5 bars, respectively. In general, long and frequent feeding events associated with the acquisition and inoculation of X. fastidiosa were observed only in fully irrigated plants (i.e., >-10 bars), which suggests that even low levels of plant water stress may reduce the spread of X. fastidiosa. Results provided insights to disease epidemiology and support the hypothesis that application of regulated deficit irrigation has the potential to reduce the incidence of diseases caused by X. fastidiosa by reducing the number of vectors and by decreasing pathogen transmission efficiency. C1 [Krugner, Rodrigo; Backus, Elaine A.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Krugner, R (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM Rodrigo.Krugner@ars.usda.gov NR 43 TC 3 Z9 3 U1 2 U2 24 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 66 EP 74 DI 10.1603/EC13219 PG 9 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400008 PM 24665686 ER PT J AU Obra, GB Resilva, SS Follett, PA Lorenzana, LRJ AF Obra, Glenda. B. Resilva, S. S. Follett, P. A. Lorenzana, L. R. J. TI Large-Scale Confirmatory Tests of a Phytosanitary Irradiation Treatment Against Sternochetus frigidus (Coleoptera: Curculionidae) in Philippine Mango SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Sternochetus frigidus; mango pulp weevil; postharvest phytosanitary treatment; quarantine pest; disinfestation ID GREEN STINK BUG; CALOCORIS-NORVEGICUS HEMIPTERA; EPICARP LESION; ACROSTERNUM-HILARE; NEZARA-VIRIDULA; PISTACHIO FRUIT; SEASONAL PHENOLOGY; PENTATOMIDAE; HETEROPTERA; BIOLOGY AB The mango pulp weevil, Sternochetus frigidus (F.), is an important quarantine pest preventing the export of mangoes from the Philippines to the United States and other countries. Previously, a radiation dose of 100 Gy was proposed for phytosanitary treatment of S. frigidus based on dose response studies with larvae, pupae, and adult weevils. To validate an irradiation treatment, large-scale confirmatory tests were conducted with adults (the most radiation-tolerant stage) in mangoes at 100 and 150 Gy. After treatment, adults were removed from fruit, sexed, and mated in pairs to observe any reproduction. At 100 Gy, adults laid a small number of eggs but none of the eggs hatched. At 150 Gy (measured doses 96.7-164.1 Gy), 4,559 treated weevils laid no eggs, indicating that this dose caused complete sterility. Irradiation treatment with a minimum absorbed dose of 165 Gy will therefore provide quarantine security for S. frigidus in exported Philippine mangoes. C1 [Obra, Glenda. B.; Resilva, S. S.] Philippine Nucl Res Inst, Dept Sci & Technol, Quezon City 1101, Philippines. [Follett, P. A.] USDA ARS, US Pacifc Basin Agr Res Ctr, Hilo, HI 96720 USA. [Lorenzana, L. R. J.] Dept Agr, Regional Field Unit 4B, Lipa City 4217, Batangas, Philippines. RP Obra, GB (reprint author), Philippine Nucl Res Inst, Dept Sci & Technol, Commonwealth Ave, Quezon City 1101, Philippines. EM gbobra@pnri.dost.gov.ph FU California Pistachio Commission; Almond Board of California FX We thank Glenn Yokota and John Andrews for insectary help; Erik Davidian, Patrick Halbig, Shanthi Kappagoda, and Nicole Korfanta for laboratory assistance; and Robert Beede, Walt Bentley, Brent Holtz, Daryl Castro, Kevin Olson, and Gary Weinberger for project development and help with field collections of insects. Funds were provided by the California Pistachio Commission and Almond Board of California are gratefully acknowledged. NR 45 TC 2 Z9 2 U1 1 U2 3 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 161 EP 173 DI 10.1603/EC13316 PG 13 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400020 PM 24665698 ER PT J AU Mayfield, AE Fraedrich, SW Taylor, A Merten, P Myers, SW AF Mayfield, A. E., III Fraedrich, S. W. Taylor, A. Merten, P. Myers, S. W. TI Efficacy of Heat Treatment for the Thousand Cankers Disease Vector and Pathogen in Small Black Walnut Logs SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE debarking; Geosmithia morbida; phytosanitary treatment; Pityophthorus juglandis; walnut twig beetle ID EMERALD ASH BORER; PITYOPHTHORUS-JUGLANDIS; TWIG BEETLE; UNITED-STATES; COLEOPTERA-SCOLYTIDAE; GEOSMITHIA-MORBIDA; MORTALITY; LEPIDOPTERA; TEMPERATURE; BUPRESTIDAE AB Thousand cankers disease, caused by the walnut twig beetle (Pityophthorus juglandis Blackman) and an associated fungal pathogen (Geosmithia morbida M. Kolarik, E. Freeland, C. Utley, and N. Tisserat), threatens the health and commercial use of eastern black walnut (Juglans nigra L.), one of the most economically valuable tree species in the United States. Effective phytosanitary measures are needed to reduce the possibility of spreading this insect and pathogen through wood movement. This study evaluated the efficacy of heat treatments and debarking to eliminate P. juglandis and G. morbida in J. nigra logs 4-18 cm in diameter and 30 cm in length. Infested logs were steam heated until various outer sapwood temperatures (60, 65, and 70 degrees C in 2011; 36, 42, 48, 52, and 56 degrees C in 2012) were maintained or exceeded for 30-40 min. In 2011, all heat treatments eliminated G. morbida from the bark, but logs were insufficiently colonized by P. juglandis to draw conclusions about treatment effects on the beetle. Debarking did not ensure elimination of the pathogen from the sapwood surface. In 2012, there was a negative effect of increasing temperature on P. juglandis emergence and G. morbida recovery. G. morbida did not survive in logs exposed to treatments in which minimum temperatures were 48 degrees C or higher, and mean P. juglandis emergence decreased steadily to zero as treatment minimum temperature increased from 36 to 52 degrees C. A minimum outer sapwood temperature of 56 degrees C maintained for 40 min is effective for eliminating the thousand cankers disease vector and pathogen from walnut logs, and the current heat treatment schedule for the emerald ash borer (60 degrees C core temperature for 60 min) is more than adequate for treating P. juglandis and G. morbida in walnut firewood. C1 [Mayfield, A. E., III] USDA, Forest Serv, Southern Res Stn, Asheville, NC 28804 USA. [Fraedrich, S. W.] USDA, Forest Serv, Southern Res Stn, Athens, GA 30602 USA. [Taylor, A.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Merten, P.] USDA, Forest Serv, Asheville, NC 28804 USA. [Myers, S. W.] USDA APHIS, Ctr Plant Hlth Sci & Technol, Buzzards Bay, MA 02542 USA. RP Mayfield, AE (reprint author), USDA, Forest Serv, Southern Res Stn, 200 WT Weaver Blvd, Asheville, NC 28804 USA. EM amayfield02@fs.fed.us FU USDA Forest Service (USDA-FS) Forest Health Protection; USDA-FS Southern Research Station; USDA-APHIS; University of Tennessee FX We gratefully acknowledge the following individuals and agencies for their assistance with this project: Daren Bailey and the Tennessee Division of Forestry, Knoxville Office; Dillion Alley, Davis Maclemore, and Coskun Kose (University of Tennessee-Center for Renewable Carbon); Sian Bailey and Peggy Elder (USDA APHIS); Susan Best, William Elliott, and William Sweeney (USDA Forest Service, Southern Research Station); Andrew Tait, Ashley Hancock, Dustin Neufeld, Corinne Fretwell, and Barbara Reynolds (University of North Carolina at Asheville); Joseph Loudermilk (Haywood Community College); Cortese Tree Specialists; Mencers Tree Specialists; City of Knoxville Lakeshore Park; Knox County Parks and Recreation; Katheryne Nix, Mark Windham, and Paris Lambdin (University of Tennessee-Entomology and Plant Pathology Department); and several anonymous homeowners in Knox County, TN. Special thanks to Cavell Brownie (North Carolina State University, emeritus professor) for help with statistical analyses. This work was funded by USDA Forest Service (USDA-FS) Forest Health Protection, USDA-FS Southern Research Station, USDA-APHIS, and the University of Tennessee. NR 38 TC 4 Z9 5 U1 1 U2 24 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 174 EP 184 DI 10.1603/EC13390 PG 11 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400021 PM 24665700 ER PT J AU Lopez-Martinez, G Carpenter, JE Hight, SD Hahn, DA AF Lopez-Martinez, Giancarlo Carpenter, James E. Hight, Stephen D. Hahn, Daniel A. TI Low-Oxygen Atmospheric Treatment Improves the Performance of Irradiation-Sterilized Male Cactus Moths Used in SIT SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE gamma radiation; oxidative damage; antioxidant; hormesis; anoxia ID CACTOBLASTIS-CACTORUM LEPIDOPTERA; FRUIT-FLY DIPTERA; GAMMA-IRRADIATION; CODLING MOTH; SEXUAL COMPETITIVENESS; INHERITED STERILITY; INSECT TECHNIQUE; F-1 STERILITY; PYRALIDAE; TEPHRITIDAE AB As part of sterile insect technique (SIT) programs, irradiation can effectively induce sterility in insects by damaging genomic DNA. However, irradiation also induces other off-target side effects that reduce the quality and performance of sterilized males. Thus, treatments that reduce off-target effects of irradiation on male performance while maintaining sterility can improve the feasibility and economy of SIT programs. Exposure to ionizing radiation induces the formation of damaging free radicals in biological systems that may reduce sterile male performance. Here, we test whether exposure to an anoxic environment for 1 h before and during irradiation improves male performance, while maintaining sterility in males of the cactus moth, Cactoblastis cactorum (Berg). We show that exposure to 1 h of anoxia increases the moth's antioxidant capacity and that irradiation in anoxia after 1h of anoxic conditioning decreases irradiation-induced oxidative damage to the moth's lipids and proteins. Anoxia treatment that reduced oxidative damage after irradiation also produced moths with greater flight performance, mating success, and longevity, while maintaining F 1 male sterility at acceptable levels for SIT. We conclude that anoxia pretreatment followed by irradiation in anoxia is an efficient way to improve the quality of irradiated moths and perhaps lower the number of moths needed for release SIT moth operations. C1 [Lopez-Martinez, Giancarlo; Hahn, Daniel A.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Lopez-Martinez, Giancarlo] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Carpenter, James E.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Hight, Stephen D.] Florida A&M Univ, USDA ARS CMAVE, Tallahassee, FL 32308 USA. RP Lopez-Martinez, G (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. EM gclopez@nmsu.edu OI Lopez-Martinez, Giancarlo/0000-0002-7937-5002 FU USDA-NIFA [2011-67012-30671]; [USDA-TSTARc-0905 1246] FX This research was funded by USDA-TSTARc-0905 1246 to D.A.H. and USDA-NIFA 2011-67012-30671 to G.L.M. We thank George Schneider, Suzanne Fraser, and Carl Gillis from the Florida Department of Agriculture and Consumer Services (FDACS-DPI), and Robert Caldwell and Susan Drawdy from the USDA-ARS Crop Protection and Management Research Unit in Tifton, GA, for providing us with cactus moths and irradiation assistance. In addition, we thank Sabrina A. White and Theodore R. Cogley with all their help with the sterility and performance experiments. NR 42 TC 4 Z9 4 U1 1 U2 25 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 185 EP 197 DI 10.1603/EC13370 PG 13 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400022 PM 24665701 ER PT J AU Sisterson, MS AF Sisterson, Mark S. TI Evaluation of a Method to Quantify Glassy-Winged Sharpshooter (Hemiptera: Cicadellidae) Egg Maturation During a Feeding Assay SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Xylella fastidiosa; Pierce's disease; ovigeny; vector ecology; Cicadellidae ID HOMALODISCA-COAGULATA HEMIPTERA; LEAF SCORCH DISEASE; XYLELLA-FASTIDIOSA; RESOURCE-ALLOCATION; SAY HEMIPTERA; VITRIPENNIS; TRANSMISSION; CALIFORNIA; LEAFHOPPER; VECTOR AB A method to improve an assay relating adult feeding to egg maturation by the glassy-winged sharpshooter (Hemiptera: Cicadellidae) was evaluated. The assay consisted on confining females to cowpea stems and quantifying feeding and egg maturation. Feeding was quantified by measuring excreta production. The number of eggs matured during the assay was estimated by taking the difference between female egg load (number of mature eggs carried by a female) at end of the assay (determined by dissection) and mean egg load of a subset of females dissected at start of the assay. Estimates of the number of mature eggs produced by females using the aforementioned approach improve as variability in egg loads of females entering the assay declines. As egg loads of females are variable, a pretreatment designed to reduce variance in egg loads of females entering the assay was evaluated. To accomplish this, females were divided into two groups. The control group was placed directly into the assay. The pretreatment group was given an oviposition period on sorghum before the assay. An oviposition period on sorghum was expected to reduce variance in egg load among females, as previous research found that sorghum was suitable for oviposition but provided poor nutrition for egg maturation. Dissection of a subset of females from each group before the assay determined that the mean and variance in egg load of females receiving the pretreatment was significantly reduced compared with females in the control group. Analysis of results from the feeding assay found that there was a significant relationship between feeding and egg maturation for females receiving the pretreatment, but not for females in the control group. Thus, reducing the mean and variance in egg load of females entering feeding assays resulted in detection of a significant positive relationship between feeding and egg maturation that otherwise would not have been observed. C1 USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Sisterson, MS (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM mark.sisterson@ars.usda.gov NR 43 TC 3 Z9 3 U1 3 U2 6 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 206 EP 214 DI 10.1603/EC13136 PG 9 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400024 PM 24665703 ER PT J AU Hoffmann, AM French, BW Jaronski, ST Gassmann, AJ AF Hoffmann, Amanda M. French, B. Wade Jaronski, Stefan T. Gassmann, Aaron J. TI Effects of Entomopathogens on Mortality of Western Corn Rootworm (Coleoptera: Chrysomelidae) and Fitness Costs of Resistance to Cry3Bb1 Maize SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE biological control; Diabrotica virgifera virgifera; fungi; nematode; refuge strategy ID DIABROTICA-VIRGIFERA-VIRGIFERA; PINK-BOLLWORM RESISTANCE; BACILLUS-THURINGIENSIS; BIOLOGICAL-CONTROL; BT MAIZE; INSECT RESISTANCE; TOXIN CRY1AC; NEMATODES; FUNGI; ADAPTATION AB Fitness costs can delay pest resistance to crops that produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt), and past research has found that entomopathogens impose fitness costs of Bt resistance. In addition, entomopathogens can be used for integrated pest management by providing biological control of pests. The western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a major pest of maize and is currently managed by planting of Bt maize. We tested whether entomopathogenic nematodes and fungi increased mortality of western corn rootworm and whether these entomopathogens increased fitness costs of resistance to Cry3Bb1 maize. We exposed western corn rootworm larvae to two species of nematodes, Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhabditidae) and Steinernema feltiae Filipjev (Rhabditida: Steinernematidae), and to two species of fungi, Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) (strain GHA) and Metarhizium brunneum (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) (strain F52) in two assay types, namely, seedling mat and small cup. Larval mortality increased with the concentration of H. bacteriophora and S. feltiae in the small cup assay, and with the exception of S. feltiae and B. bassiana in the seedling mat assay, mortality from entomopathogens was significantly greater than zero for the remaining entomopathogens in both assays. However, no fitness costs were observed in either assay type for any entomopathogen. Increased mortality of western corn rootworm larvae caused by these entomopathogens supports their potential use in biological control; however, the lack of fitness costs suggests that entomopathogens will not delay the evolution of Bt resistance in western corn rootworm. C1 [Hoffmann, Amanda M.; Gassmann, Aaron J.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [French, B. Wade] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Jaronski, Stefan T.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Hoffmann, AM (reprint author), Iowa State Univ, Dept Entomol, 110 Insectary Bldg, Ames, IA 50011 USA. EM iahoff17@gmail.com FU USDA National Institute of Food and Agriculture [2009-33120-20256] FX Sean Bradley assisted with experiments. This research was supported by Biotechnology Risk Assessment Grant Program competitive grant 2009-33120-20256 from the USDA National Institute of Food and Agriculture. NR 50 TC 8 Z9 9 U1 4 U2 40 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 352 EP 360 DI 10.1603/EC13247 PG 9 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400041 PM 24665720 ER PT J AU Binning, RR Coats, J Kong, XX Hellmich, RL AF Binning, Rachel R. Coats, Joel Kong, Xiaoxiao Hellmich, Richard L. TI Susceptibility and Aversion of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Cry1F Bt Maize and Considerations for Insect Resistance Management SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE event TC1507; larval movement; transgenic corn; antixenosis ID FIELD-RESISTANCE; FEEDING-BEHAVIOR; PUERTO-RICO; ADAPTATION; COTTON; LARVAE; ALLELOCHEMICALS; HABITUATION; CONSUMPTION; DETERRENT AB Bacillus thuringiensis (Bt) maize was developed primarily for North American pests such as European corn borer (Ostrinia nubilalis (Hubner)). However, most Bt maize products are also cultivated outside of North America, where the primary pests may be different and may have lower susceptibility to Bt toxins. Fall armyworm (Spodoptera frugiperda JE Smith) is an important pest and primary target of Bt maize in Central and South America. S. frugiperda susceptibility to Cry1F (expressed in event TC1507) is an example of a pest-by-toxin interaction that does not meet the high-dose definition. In this study, the behavioral and toxic response of S. frugiperda to Cry1F maize was investigated by measuring the percentage of time naive third instars spent feeding during a 3-min exposure. S. frugiperda also were exposed as third instars to Cry1F maize for 14 d to measure weight gain and survival. S. frugiperda demonstrated an initial, postingestive aversive response to Cry1F maize, and few larvae survived the 14 d exposure. The role of susceptibility and avoidance are discussed in the context of global IRM refuge strategy development for Bt products. C1 [Binning, Rachel R.] DuPont Pioneer, Johnston, IA 50131 USA. [Coats, Joel] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Kong, Xiaoxiao] DuPont Pioneer, Ankeny, IA 50021 USA. [Hellmich, Richard L.] Iowa State Univ, USDA ARS, Genet Lab 110, Ames, IA 50011 USA. RP Binning, RR (reprint author), DuPont Pioneer, 7250 NW 62nd Ave,POB 552, Johnston, IA 50131 USA. EM rachel.binning@pioneer.com NR 25 TC 7 Z9 7 U1 2 U2 32 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 368 EP 374 DI 10.1603/EC13352 PG 7 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400043 PM 24665722 ER PT J AU Aluja, M Arredondo, J Diaz-Fleischer, F Birke, A Rull, J Niogret, J Epsky, N AF Aluja, M. Arredondo, J. Diaz-Fleischer, F. Birke, A. Rull, J. Niogret, J. Epsky, N. TI Susceptibility of 15 Mango (Sapindales: Anacardiaceae) Cultivars to the Attack by Anastrepha ludens and Anastrepha obliqua (Diptera: Tephritidae) and the Role of Underdeveloped Fruit as Pest Reservoirs: Management Implications SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Anastrepha ludens; Anastrepha obliqua; mango; resistance; trap cropping ID CERATITIS-CAPITATA DIPTERA; HOST MARKING PHEROMONE; MANGIFERA-INDICA L.; FLY DIPTERA; POPULATION FLUCTUATIONS; VOLATILE COMPONENTS; MAGGOT DIPTERA; TROPICAL PLUM; FLIES DIPTERA; INFESTATION AB We evaluated the susceptibility of 15 mango cultivars to the attack of Anastrepha ludens (Loew) and Anastrepha obliqua (Macquart) (Diptera: Tephritidae), the main tephritid pests of this crop in Mexico. In a field experiment, bagged fruit-bearing branches were exposed to gravid females of both fly species. Infestation rates, developmental time, adult eclosion, and F1 adult longevity, fecundity, and fertility were recorded, ranking cultivars in terms of susceptibility to fly attack and development. We also compared the volatile profile in selected resistant and susceptible cultivars in search of possible correlations. In a second experiment, clutch size for A. ludens was determined in each cultivar. Infestation rates, developmental time, and F1 demographic parameters varied sharply among cultivars and between fly species for bagged fruit. Cultivars 'Vishi,' '74-82,' and 'Brooks' were most susceptible to A. ludens infestation while 'Tommy,' 'Sensation,' and 'Ataulfo "nino"' (parthenocarpic fruit) were most susceptible to A. obliqua infestation. 'Edward,' 'Kent,' 'Brooks late,' 'Palmer,' and 'Ataulfo' exhibited tolerance to attack of both fly species. Fruit of susceptible and resistant cultivars exhibited unique volatile profiles. Fly development and F1 adult demographic parameters varied significantly among cultivars. A. ludens females laid larger clutches in larger and harder fruit. We highlight the important role of Ataulfo "nino" as pest reservoir if fruit is left unharvested on trees. We discuss the possible use of highly resistant cultivars as trap crops or egg sinks. C1 [Aluja, M.; Birke, A.; Rull, J.] Inst Ecol AC, Red Manejo Biorrac Plagas & Vectores, Xalapa 91000, Veracruz, Mexico. [Arredondo, J.; Diaz-Fleischer, F.] Programa Moscafrut SAGARPA IICA, Metapa De Domonguez 30680, Chiapas, Mexico. [Diaz-Fleischer, F.] Univ Veracruzana, IN BIOTECA, Xalapa 91000, Veracruz, Mexico. [Niogret, J.; Epsky, N.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. RP Aluja, M (reprint author), Inst Ecol AC, Red Manejo Biorrac Plagas & Vectores, Apartado Postal 63, Xalapa 91000, Veracruz, Mexico. EM martin.aluja@inecol.edu.mx RI Diaz-Fleischer, Francisco/N-1275-2016 OI Diaz-Fleischer, Francisco/0000-0003-2137-6587 FU Mexican Campana Nacional contra Moscas de la Fruta (Convenio SAGARPA-IICA-INECOL); Instituto de Ecologia, A.C. FX We are thankful to Alvaro Meza, Fernando Avendano, and Adan Ochoa for technical assistance, and to MC Victor Palacio Martinez, Victor Hugo Diaz Fuente, Alfredo Sandoval Esquivez, and Juan F. Aguirre Medina of INIFAP; Mike Winterstein, David Kuhn, Tomas Ayala-Silva (USDA-ARS, Miami, FL); and Jonathan Crane (University of Florida-Tropical Research and Education Center [UF-TREC], Homestead, FL) for cultivar information and use of their orchards. We also thank Pablo Montoya and Larissa Guillen for their critical support at various stages of the research project. We sincerely appreciate the insightful and critical comments to an earlier version of this manuscript of two anonymous referees, which greatly improved its quality. Funds were provided by the Mexican Campana Nacional contra Moscas de la Fruta (Convenio SAGARPA-IICA-INECOL), and the Instituto de Ecologia, A.C. NR 73 TC 5 Z9 6 U1 0 U2 17 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 375 EP 388 DI 10.1603/EC13045 PG 14 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400044 PM 24665723 ER PT J AU Currie, Y Moch, J Underwood, J Kharabsheh, H Quesenberry, A Miyagi, R Thomas, C Boney, M Woods, S Chen, MS Zhu, LC AF Currie, Yaleaka Moch, John Underwood, Joshua Kharabsheh, Hamzah Quesenberry, Amy Miyagi, Risa Thomas, Carolyn Boney, Melanie Woods, Samantha Chen, Ming-Shun Zhu, Lieceng TI Transient Heat Stress Compromises the Resistance of Wheat (Poales: Poaceae) Seedlings to Hessian Fly (Diptera: Cecidomyiidae) Infestation SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE heat stress; wheat; Hessian fly; loss of resistance; phytohormone ID MAYETIOLA-DESTRUCTOR; PLANT DEFENSE; SHOCK PROTEINS; FATTY-ACIDS; TEMPERATURE; PHYTOHORMONES; ARABIDOPSIS; THERMOTOLERANCE; EXPRESSION; TOLERANCE AB Heat stress exerts a profound impact on the resistance of plants to parasites. In this research, we investigated the impact of an acute transient heat stress on the resistance of the wheat line 'Molly,' which contains the R gene H13, to an avirulent Hessian fly (Mayetiola destructor (Say)) population. We found that a significant portion of Molly seedlings stressed at 40 degrees C for 6 h during or after the initial Hessian fly larval attack became susceptible to otherwise avirulent insects, whereas unstressed control plants remained 100% resistant. Specifically, 77.8, 73.3, 83.3, and 46.7% of plants heat stressed at 0, 6, 12, and 24 h, respectively, after the initial larval attack became susceptible. Biochemical analysis revealed that heat stress caused a transient decrease in 12-oxo-phytodienoic acid, but an increase in salicylic acid accumulation in Molly plants. The change in phytohormones after heat stress and Hessian fly infestation was not observed in 'Newton,' a near-isogenic but Hessian fly susceptible wheat line. Instead, heat stress caused a relatively prolonged reduction in palmitoleic acid. The role of phytohormones in heat-induced loss of wheat resistance was discussed. C1 [Currie, Yaleaka; Moch, John; Underwood, Joshua; Kharabsheh, Hamzah; Quesenberry, Amy; Miyagi, Risa; Thomas, Carolyn; Boney, Melanie; Woods, Samantha; Zhu, Lieceng] Fayetteville State Univ, Dept Biol Sci, Fayetteville, NC 28301 USA. [Chen, Ming-Shun] Kansas State Univ, Dept Entomol, Hard Winter Wheat Genet Res Unit, Ctr Grain & Anim Hlth Res,USDA ARS, Manhattan, KS 66506 USA. RP Zhu, LC (reprint author), Fayetteville State Univ, Dept Biol Sci, 1200 Murchison Rd, Fayetteville, NC 28301 USA. EM lzhu@uncfsu.edu FU Fayetteville State University; National Science Foundation HRD Award [1036257] FX We thank Thilani Samarakoon in Kansas Lipidomics Research Center at Kansas State University for profiling phytohormones and fatty acids. This research was supported by 2012 and 2013 Fayetteville State University Title III HBCU Masters Degree STEM Program Faculty Research Mini-Grants, 2012 Summer Faculty Research Stipend Program, and a grant from the National Science Foundation HRD Award #1036257. NR 38 TC 3 Z9 3 U1 1 U2 13 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 389 EP 395 DI 10.1603/EC13261 PG 7 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400045 PM 24665724 ER PT J AU Garces-Carrera, S Knutson, A Wang, H Giles, KL Huang, FN Whitworth, RJ Smith, CM Chen, MS AF Garces-Carrera, Sandra Knutson, Allen Wang, Haiyan Giles, Kristopher L. Huang, Fangneng Whitworth, R. Jeffrey Smith, C. Michael Chen, Ming-Shun TI Virulence and Biotype Analyses of Hessian Fly (Diptera: Cecidomyiidae) Populations From Texas, Louisiana, and Oklahoma SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Mayetiola destructor; Hessian fly; biotype; wheat; plant resistance ID WINTER-WHEAT; RESISTANCE GENES; UNITED-STATES; INSECTICIDES; SOUTHEASTERN AB Hessian fly, Mayetiola destructor (Say, 1817), is a major pest of wheat, and is controlled mainly through deploying fly-resistant wheat cultivars. The challenge for the plant resistance approach is that virulence of Hessian fly populations in the field is dynamic, and wheat cultivars may lose resistance within 6-8 yr. To ensure continuous success of host plant resistance, Hessian fly populations in the field need to be constantly monitored to determine which resistance genes remain effective in different geographic regions. This study investigated five Hessian fly populations collected from Texas, Louisiana, and Oklahoma, where infestation by Hessian fly has been high in recent years. Eight resistance genes, H12, H13, H17, H18, H22, H25, H26, and Hdic, were found to be highly effective against all tested Hessian fly populations in this region, conferring resistance to >= 80% of plants containing one of these resistance genes. The frequencies of biotypes virulent to resistance genes H13 (biotype vH13), H18 (vH18), H21 (vH21), H25 (vH25), H26 (vH26), and Hdic (vHdic) were determined, and were found to vary from population to population, ranging from 0 to 45%. A logistic regression model was established to predict biotype frequencies based on the correlation between the percentages of susceptible plants obtained in a virulence test and the log-odds of virulent biotype frequencies determined by a traditional approach. C1 [Garces-Carrera, Sandra; Whitworth, R. Jeffrey; Smith, C. Michael; Chen, Ming-Shun] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Knutson, Allen] Texas A&M Univ, Texas AgriLife Res & Extens Ctr, Dept Entomol, Dallas, TX 75252 USA. [Wang, Haiyan] Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA. [Giles, Kristopher L.] Oklahoma State Univ, Dept Plant Pathol & Entomol, Stillwater, OK 74078 USA. [Huang, Fangneng] Louisiana State Univ, Dept Entomol, Agr Ctr, Baton Rouge, LA 70803 USA. [Chen, Ming-Shun] USDA ARS, Hard Winter Wheat Genet Res Unit, Ctr Grain & Anim Hlth, Manhattan, KS USA. RP Chen, MS (reprint author), Kansas State Univ, Dept Entomol, 123 Waters Hall, Manhattan, KS 66506 USA. EM mchen@ksu.edu FU USDA-NIFA-RAMP (U.S. Department of Agriculture-National Institute of Food and Agriculture-Risk Avoidance and Mitigation) [2010-51101-21642]; Government of Ecuador; Ecuador National Research Agriculture Institute (INIAP) FX This research was partially supported by the grant USDA-NIFA-RAMP #2010-51101-21642 (U.S. Department of Agriculture-National Institute of Food and Agriculture-Risk Avoidance and Mitigation). The Government of Ecuador and the Ecuador National Research Agriculture Institute (INIAP) supported Sandra Garces-Carrera financially while she was at KSU to pursue her PhD. NR 29 TC 7 Z9 7 U1 1 U2 11 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 417 EP 423 DI 10.1603/EC13372 PG 7 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400049 PM 24665728 ER PT J AU Koch, KG Fithian, R Heng-Moss, TM Bradshaw, JD Sarath, G Spilker, C AF Koch, Kyle G. Fithian, Rachael Heng-Moss, Tiffany M. Bradshaw, Jeff D. Sarath, Gautam Spilker, Courtney TI Evaluation of Tetraploid Switchgrass (Poales: Poaceae) Populations for Host Suitability and Differential Resistance to Four Cereal Aphids SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE switchgrass; Panicum virgatum; Sipha flava; Schizaphis graminum; plant resistance ID YELLOW SUGARCANE APHID; HOMOPTERA; GRASSES; BIOMASS; HETEROSIS; HEMIPTERA; ARMYWORM; BIOLOGY AB Switchgrass, Panicum virgatum L., is being developed as a bioenergy feedstock. The potential for large-scale production has encouraged its evaluation as a host for important grass pests. Eight no-choice studies were performed for two developmental stages of two switchgrass cultivars ('Kanlow' and 'Summer') and two experimental strains, K x S, and S x K produced by reciprocal mating of these cultivars followed by selection for high yield. Plants were evaluated for host suitability and damage differences to herbivory by four important cereal aphids, Sipha flava (Forbes), Schizaphis graminum (Rondani) (biotype I), Rhopalosiphum padi (L.), and Diuraphis noxia (Mordvilko). All switchgrasses were found to be unsuitable feeding and reproductive hosts to R. padi and D. noxia, which were unable to establish on the plants. However, both S. flava and S. graminum were able to establish on all switchgrasses tested. Differential levels of resistance to S. flava and S. graminum were detected among the switchgrasses by both cumulative aphid days (CAD) and plant damage ratings. Kanlow was consistently rated as highly resistant based on CAD and damage ratings for both aphid species, while Summer was consistently among the most susceptible to both aphids at both developmental stages, with relatively high damage ratings. The resistance of the K x S and S x K populations in relationship to their Summer and Kanlow parents indicted that they inherited some resistance to S. graminum and S. flava from their Kanlow parent. These studies provide valuable baseline information concerning the host suitability of switchgrass to four cereal aphids and the plant-insect interactions within a system that has been largely overlooked and indicate that there are genetic differences among switchgrass populations for resistance to some insects. C1 [Koch, Kyle G.; Heng-Moss, Tiffany M.; Spilker, Courtney] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Fithian, Rachael] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Bradshaw, Jeff D.] Univ Nebraska, Dept Entomol, Panhandle Res & Extens Ctr, Scottsbluff, NE 69361 USA. [Sarath, Gautam] Univ Nebraska, Grain Forage & Bioenergy Res Unit, USDA ARS, Lincoln, NE 68583 USA. RP Heng-Moss, TM (reprint author), Univ Nebraska, Dept Entomol, 103 Entomology Hall, Lincoln, NE 68583 USA. EM thengmoss2@unl.edu FU U.S. Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) [2011-67009-30096]; USDA-Agriculture Research Service Current Research Information Service [5440-21000-030-00D] FX We gratefully acknowledge Z. B. Mayo and K. P. Vogel for reviewing the manuscript; T. Prochaska, R. Patrick, and S. Harvey (University of Nebraska) for technical assistance; and K. P. Vogel for providing the switchgrass germplasm for these studies. This work was supported in part by U.S. Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) grant 2011-67009-30096, and by the USDA-Agriculture Research Service Current Research Information Service project 5440-21000-030-00D. NR 35 TC 6 Z9 6 U1 3 U2 15 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 424 EP 431 DI 10.1603/EC13315 PG 8 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400050 PM 24665729 ER PT J AU Campbell, JF Arthur, FH Zhu, KY AF Campbell, James F. Arthur, Frank H. Zhu, Kun Yan TI Spatial Pattern in Aerosol Insecticide Deposition Inside a Flour Mill SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE confused flour beetle; flour mill; aerosol; spatial pattern; dispersion ID CAGED ADULT MOSQUITOS; TRIBOLIUM-CASTANEUM; ESFENVALERATE AEROSOL; SPRAY DROPLETS; VECTOR CONTROL; EFFICACY; VOLUME; SIZE; MALATHION; PENETRATION AB Aerosol insecticides are commonly used for management of stored-product pests inside food facilities, but the physical complexity of the interior of most food facilities may influence the dispersal and deposition of droplets and create spatial variation in dosage. The spatial pattern in aerosol deposition was evaluated inside a flour mill using a high density grid of bioassay insects. Three insecticides, Aerotech with NyGuard and Pyrocide 100 + Diacon II, both pyrethrin and insect growth regulator combinations, and Vap20, an organophosphate, were evaluated at normal (27 degrees C) and high (40 degrees C) target temperatures. Using a newly developed efficacy index, there was spatial pattern to aerosol deposition detected for the pyrethrin insecticides and the pattern differed between aerosols and temperatures. Walls and corners, especially behind the direction of application, were especially vulnerable to zones of lower efficacy, and open areas in center of the room tended to have the highest efficacy. The organophosphate insecticide provided complete efficacy at all bioassay dish locations, probably due in part to its vapor toxicity. Using boxes 1 m in depth and 5, 10, or 20 cm in height and open on only one end, it was shown that aerosol dispersal under horizontal surfaces could be impacted by gap height, aerosol, and temperature. These results show the potential for spatial variation in aerosol efficacy within a food facility and identify locations with the potential to receive a lower insecticide dosage. To increase overall efficacy, these locations may be targeted for additional interventions to provide more uniform pest population suppression. C1 [Campbell, James F.; Arthur, Frank H.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Zhu, Kun Yan] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RP Campbell, JF (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM james.campbell@ars.usda.gov FU U.S. Department of Agriculture, National Institute of Food and Agriculture (NIFA) Methyl Bromide Transitions program [2010-51102-21660] FX We greatly appreciate and thank Rich Hammel, Brian Barnett, Kabita Kharel, Karrie Buckman, Laura Starkus, and Konner Cool for their technical assistance, Industrial Fumigant Company, especially Chelle Hartzer and Bill Craig, for performing the aerosol treatments, and Gerald Ducatte from MRIGlobal for performing the aerosol droplet size measurements. This research was partially funded by the U.S. Department of Agriculture, National Institute of Food and Agriculture (NIFA) Methyl Bromide Transitions program (grant 2010-51102-21660). This manuscript is contribution no. 14-091-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS. NR 43 TC 5 Z9 6 U1 1 U2 4 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 440 EP 454 DI 10.1603/EC13423 PG 15 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400052 PM 24665731 ER PT J AU Kharel, K Arthur, FH Zhu, KY Campbell, JF Subramanyam, B AF Kharel, Kabita Arthur, Frank H. Zhu, Kun Yan Campbell, James F. Subramanyam, Bhadriraju TI Evaluation of Synergized Pyrethrin Aerosol for Control of Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Tribolium castaneum; Tribolium confusum; synergized pyrethrin; aerosol; flour mill ID STORED-PRODUCT INSECTS; FLOUR MILLS; METHYL-BROMIDE; LIFE STAGES; FUMIGATION; EFFICACY; PHOSPHINE; TOXICITY; POPULATIONS; CYFLUTHRIN AB Aerosol insecticides are being used in flour mill pest management programs, but there is limited information on their efficacy on different insect life stages. In this study, we evaluated the efficacy of synergized pyrethrin applied as an aerosol against eggs, larvae, pupae, and adults of the red flour beetle, Tribolium castaneum (Herbst), and the confused flour beetle, Tribolium confusum Jacquelin du Val. Effects of direct and indirect exposure were evaluated by exposing each life stage to the aerosol and then transferring to untreated flour, transferring untreated insects to treated flour, or exposing both the insects and the flour to the aerosol. The aerosol produced >88% mortality of both species and all life stages when insects were directly treated and transferred to either treated or untreated flour. Mortality was significantly reduced when insects were either treated together with flour or untreated insects were transferred to treated flour (indirect exposure to the aerosol). Larvae and adults of both species were more tolerant compared with eggs and pupae. Recovery of moribund adults in the indirect exposure treatments was greater compared with recovery of moribund insects in the direct exposure treatments. Good sanitation before aerosol application could facilitate direct exposure of insects and thus increase aerosol efficacy inside flour mills. C1 [Kharel, Kabita; Zhu, Kun Yan] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Arthur, Frank H.; Campbell, James F.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Subramanyam, Bhadriraju] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. RP Arthur, FH (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM frank.arthur@ars.usda.gov FU U.S. Department of Agriculture, National Institute of Food and Agriculture (NIFA) Methyl Bromide Transitions program [2010-51102-21660] FX We would like to express our sincere thanks to Brian Barnett, Rich Hammel, and Kris Hartzer(USDA-ARS Center for Grain and Animal Health Research, Manhattan, KS) for their technical assistance. We appreciate Entech Corporation providing the insecticide used in this study. This research was partially supported by the U.S. Department of Agriculture, National Institute of Food and Agriculture (NIFA) Methyl Bromide Transitions program (grant 2010-51102-21660). Insect voucher specimens were deposited at the Kansas State University Museum of Entomological and Prairie Arthropod Research under the voucher no. 226. This paper is contribution number 14-029-J of the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS. NR 32 TC 6 Z9 7 U1 1 U2 5 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 462 EP 468 DI 10.1603/EC13355 PG 7 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400054 PM 24665733 ER PT J AU Perez-Mendoza, J Campbell, JF Throne, JE AF Perez-Mendoza, Joel Campbell, James F. Throne, James E. TI Effect of Abiotic Factors on Initiation of Red Flour Beetle (Coleoptera: Tenebrionidae) Flight SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE light intensity; photoperiod; relative humidity; temperature; Tribolium castaneum ID TRIBOLIUM-CASTANEUM; MATING STATUS; TEMPERATURE; BOSTRICHIDAE; FUMIGATION; HUMIDITY; MILL; AGE AB Traps baited with pheromones are used to monitor the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), populations in flour mills to aid in making pest management decisions, but the factors that influence T. castaneum movement are not fully understood. We investigated the impact of photoperiod, light intensity, temperature, and relative humidity on flight initiation. The percentage of adults initiating flight reached a maximum at 30-35 degrees C, and then fell to zero at 22.5 and 45 degrees C. Only 2% of beetles flew in complete darkness, and the number of beetles initiating flight increased to 41% under 18 h of light and then decreased slightly to 37% under 24 h of light. Rates of flight initiation did not vary with light intensities from 1,784 to 4,356 lux or relative humidities from 25 to 85%. Thus, temperature and photoperiod are the main abiotic factors tested that impact flight initiation in T. castaneum, which have broad ranges of temperatures and photoperiods over which they can fly. The current results should be useful in helping to interpret trap catches based on abiotic conditions during the trapping period, and the results should be useful in helping to understand T. castaneum movement outside grain storages and processing facilities and their potential to infest structures. C1 [Perez-Mendoza, Joel; Campbell, James F.; Throne, James E.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Throne, JE (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM james.throne@ars.usda.gov NR 17 TC 1 Z9 1 U1 0 U2 16 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2014 VL 107 IS 1 BP 469 EP 472 DI 10.1603/EC13364 PG 4 WC Entomology SC Entomology GA AA5PF UT WOS:000331151400055 PM 24665734 ER PT J AU Shen, JY Tieman, D Jones, JB Taylor, MG Schmelz, E Huffaker, A Bies, D Chen, KS Klee, HJ AF Shen, Jiyuan Tieman, Denise Jones, Jeffrey B. Taylor, Mark G. Schmelz, Eric Huffaker, Alisa Bies, Dawn Chen, Kunsong Klee, Harry J. TI A 13-lipoxygenase, TomloxC, is essential for synthesis of C5 flavour volatiles in tomato SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE C5 volatiles; hydroperoxide lyase; jasmonic acid; 13-lipoxygenase; tomato; wounding; Xanthomonas campestris pv; vesicatoria ID GREEN LEAF VOLATILE; ALLENE OXIDE SYNTHASE; ACID-DERIVED SIGNALS; ARABIDOPSIS-THALIANA; HYDROPEROXIDE LYASE; NICOTIANA-ATTENUATA; PSEUDOMONAS-SYRINGAE; JASMONIC ACID; PLANT DEFENSE; LIPOXYGENASE AB C5 volatile compounds, derived from fatty acids, are among the most important contributors to consumer liking of fresh tomatoes. Despite their important roles in flavour, the genes responsible for C5 volatile synthesis have yet to be identified. This work shows that their synthesis is catalysed in part by a 13-lipoxygenase (LOX), TomloxC, the same enzyme responsible for synthesis of C6 volatiles. C5 synthesis is independent of hydroperoxide lyase (HPL); moreover, HPL knockdown significantly increased C5 volatile synthesis. This LOX-dependent, HPL-independent pathway functions in both fruits and leaves. Synthesis of C5 volatiles increases in leaves following mechanical wounding but does not increase in response to infection with Xanthomonas campestris pv. vesicatoria. Large reductions in C5 and C6 volatiles in antisense TomloxC knockdown plants were observed but those reductions did not alter the development of disease symptoms, indicating that these volatiles do not have an important defensive function against this bacterial pathogen. C1 [Shen, Jiyuan; Tieman, Denise; Taylor, Mark G.; Bies, Dawn; Klee, Harry J.] Univ Florida, Gainesville, FL 32611 USA. [Shen, Jiyuan; Chen, Kunsong] Zhejiang Univ, Lab Fruit Qual Biol, State Dgriculture Minist, Lab Hort Plant Growth Dev & Qual Improvement, Hangzhou 310058, Zhejiang, Peoples R China. [Jones, Jeffrey B.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Schmelz, Eric; Huffaker, Alisa] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Chen, KS (reprint author), Zhejiang Univ, Lab Fruit Qual Biol, State Dgriculture Minist, Lab Hort Plant Growth Dev & Qual Improvement, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China. EM akun@zju.edu.cn; hjklee@ufl.edu RI Klee, Harry/A-7912-2008; liu, yanyang/H-4808-2016; OI Schmelz, Eric/0000-0002-2837-734X FU National Science Foundation [IOS-0923312]; Florida Agricultural Experiment Station; National Basic Research Program of China [2011CB100602]; China Scholarship Council FX The authors thank Dr Neha Potnis for critical suggestions and help for disease experiment performing and analysis. They also thank Dr. Bo Zhang for helpful comments on the manuscript. This work was supported by National Science Foundation (grant IOS-0923312 to HK) and the Florida Agricultural Experiment Station. The National Basic Research Program of China (2011CB100602) and China Scholarship Council supported JS with living expenses for his research in the USA. NR 45 TC 30 Z9 31 U1 7 U2 58 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD FEB PY 2014 VL 65 IS 2 BP 419 EP 428 DI 10.1093/jxb/ert382 PG 10 WC Plant Sciences SC Plant Sciences GA AA5EQ UT WOS:000331119300006 PM 24453226 ER PT J AU Lerman, SB Nislow, KH Nowak, DJ DeStefano, S King, DI Jones-Farrand, DT AF Lerman, Susannah B. Nislow, Keith H. Nowak, David J. DeStefano, Stephen King, David I. Jones-Farrand, D. Todd TI Using urban forest assessment tools to model bird habitat potential SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Habitat models; Urban biodiversity; i-Tree; Management; Suitability index ID SUITABILITY INDEX MODELS; BIODIVERSITY CONSERVATION; NESTING SUCCESS; SCALE HABITAT; UNITED-STATES; LAND-USE; GRADIENT; URBANIZATION; ASSOCIATIONS; COMMUNITY AB The alteration of forest cover and the replacement of native vegetation with buildings, roads, exotic vegetation, and other urban features pose one of the greatest threats to global biodiversity. As more land becomes slated for urban development, identifying effective urban forest wildlife management tools becomes paramount to ensure the urban forest provides habitat to sustain bird and other wildlife populations. The primary goal of this study was to integrate wildlife suitability indices to an existing national urban forest assessment tool, i-Tree. We quantified available habitat characteristics of urban forests for ten northeastern U.S. cities, and summarized bird habitat relationships from the literature in terms of variables that were represented in the i-Tree datasets. With these data, we generated habitat suitability equations for nine bird species representing a range of life history traits and conservation status that predicts the habitat suitability based on i-Tree data. We applied these equations to the urban forest datasets to calculate the overall habitat suitability for each city and the habitat suitability for different types of land-use (e.g., residential, commercial, parkland) for each bird species. The proposed habitat models will help guide wildlife managers, urban planners, and landscape designers who require specific information such as desirable habitat conditions within an urban management project to help improve the suitability of urban forests for birds. (C) 2013 Elsevier B.V. All rights reserved. C1 [Lerman, Susannah B.; Nislow, Keith H.; King, David I.] US Forest Serv, No Res Stn, USDA, Amherst, MA 01354 USA. [Lerman, Susannah B.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01354 USA. [Nowak, David J.] US Forest Serv, No Res Stn, USDA, Syracuse, NY USA. [DeStefano, Stephen] Univ Massachusetts, US Geol Survey, Massachusetts Cooperat Fish & Wildlife Res Unit, Amherst, MA 01354 USA. [Jones-Farrand, D. Todd] US Fish & Wildlife Serv, Columbia, MO USA. RP Lerman, SB (reprint author), US Forest Serv, No Res Stn, USDA, 160 Holdsworth Way, Amherst, MA 01354 USA. EM slerman@cns.umass.edu; knislow@fs.fed.us; dnowak@fs.fed.us; sdestef@eco.umass.edu; dking@fs.fed.us; david_jones-farrand@fws.gov FU Robert and Patricia Switzer Foundation; National Science Foundation [DEB 0423476] FX We thank Bob Hoehn, Randy Dettmers, David Small, James Smith, Marshall Iliff, Frank Thompson, and Bill DeLuca for supplying the data and assistance with assessing the habitat models. Charlie Nilon and Paige Warren assisted with the bird data from the Baltimore Ecosystem Study (BES LTER) which is supported by the National Science Foundation's Long-term Ecological Research program, grant number DEB 0423476. Bradley Blackwell, Kirsten Bryan, Adam Finkle and Sandra Haire, and three anonymous reviewers provided constructive comments on earlier drafts of the manuscript. S.B. Lerman was supported by a Switzer Environmental Leadership grant from the Robert and Patricia Switzer Foundation. NR 70 TC 3 Z9 3 U1 4 U2 66 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD FEB PY 2014 VL 122 BP 29 EP 40 DI 10.1016/j.landurbplan.2013.10.006 PG 12 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA AA3VN UT WOS:000331023100003 ER PT J AU DeVault, TL Seamans, TW Schmidt, JA Belant, JL Blackwell, BF Mooers, N Tyson, LA Van Pelt, L AF DeVault, Travis L. Seamans, Thomas W. Schmidt, Jason A. Belant, Jerrold L. Blackwell, Bradley F. Mooers, Nicole Tyson, Laura A. Van Pelt, Lolita TI Bird use of solar photovoltaic installations at US airports: Implications for aviation safety SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Airport; Bird strike; Photovoltaic; Renewable energy; Solar; US Federal Aviation Administration ID WILDLIFE CONSERVATION; ENERGY DEVELOPMENT; SEASON GRASSLANDS; UNITED-STATES; MANAGEMENT; HAZARDS; DESERT AB Several airports in the US have recently installed large photovoltaic (PV) arrays near air-operations areas to offset energy demands, and the US Federal Aviation Administration has published guidelines for new solar installations on airport properties. Although an increased reliance on solar energy will likely benefit airports from environmental and economic perspectives, bird use of solar installations should be examined before wide-scale implementation to determine whether such changes in land use adversely affect aviation safety by increasing risk of bird-aircraft collisions. We studied bird use of five pairs of PV arrays and nearby airport grasslands in Arizona, Colorado, and Ohio, over one year. Across locations, we observed 46 species of birds in airfield grasslands compared to 37 species in PV arrays. We calculated a bird hazard index (BHI) based on the mean seasonal mass of birds per area surveyed. General linear model analysis indicated that BHI was influenced by season, with higher BHI in summer than fall and winter. We found no effect of treatment (PV arrays vs. airfields), location, or interactions among predictors. However, using a nonparametric two-group test across all seasons and locations, we found greater BHI in airfield grasslands than PV arrays for those species considered especially hazardous to aircraft (species >= 1.125 kg). Our results suggest that converting airport grasslands to PV arrays would not increase hazards associated with bird-aircraft collisions. Published by Elsevier B.V. C1 [DeVault, Travis L.; Seamans, Thomas W.; Schmidt, Jason A.; Blackwell, Bradley F.; Mooers, Nicole; Tyson, Laura A.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. [Belant, Jerrold L.] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS USA. [Van Pelt, Lolita] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Sandusky, OH 44870 USA. RP DeVault, TL (reprint author), Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr,Ohio Field Stn, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM Travis.L.DeVault@aphis.usda.gov; Thomas.W.Seamans@aphis.usda.gov; JasonAlanSchmidt@hotmail.com; JBelant@cfr.msstate.edu; Bradley.F.Blackwell@aphis.usda.gov; Nicole.L.Mooers@aphis.usda.gov; Laura.A.Tyson@aphis.usda.gov; Lolita.I.VanPelt@aphis.usda.gov FU US Department of Agriculture; Federal Aviation Administration FX We thank the US Department of Agriculture and the Federal Aviation Administration for funding our research, and D. Bergman, K. Cross, A. Franklin, and S. Shriner for help with various aspects of this study. We also thank the personnel at airports and solar facilities who allowed us to access their properties to collect data. E. Poggiali provided critical logistical assistance during our study, and M. Tobin reviewed an earlier version of this manuscript. Opinions expressed in this study do not necessarily reflect current Federal Aviation Administration policy decisions regarding the control of wildlife on or near airports. NR 46 TC 5 Z9 5 U1 4 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD FEB PY 2014 VL 122 BP 122 EP 128 DI 10.1016/j.landurbplan.2013.11.017 PG 7 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA AA3VN UT WOS:000331023100011 ER PT J AU Pao, S Hagens, BE Kim, C Wildeus, S Ettinger, MR Wilson, MD Watts, BD Whitley, NC Porto-Fett, ACS Schwarz, JG Kaseloo, R Ren, S Long, W Li, H Luchansky, JB AF Pao, S. Hagens, B. E. Kim, C. Wildeus, S. Ettinger, M. R. Wilson, M. D. Watts, B. D. Whitley, N. C. Porto-Fett, A. C. S. Schwarz, J. G. Kaseloo, R. Ren, S. Long, W., III Li, H. Luchansky, J. B. TI Prevalence and molecular analyses of Campylobacter jejuni and Salmonella spp. in co-grazing small ruminants and wild-living birds SO LIVESTOCK SCIENCE LA English DT Article DE Foodborne pathogens; Feces; Farm; Sheep; Goats; Birds ID NEW-ZEALAND; THERMOPHILIC CAMPYLOBACTERS; GASTROINTESTINAL-TRACT; SLAUGHTERED SHEEP; ESCHERICHIA-COLI; CONTACT SURFACES; GREAT-BRITAIN; DEBRE-ZEIT; GOATS; CHICKEN AB A total of 689 co-grazing small ruminants along with 446 wild-living birds were tested during two springs and autumns (2007-2009) under two management systems at two Mid-Atlantic locations (similar to 187 km in aerial distance) of the U.S. Fecal shedding of Campylobacter jejuni and Salmonella spp. were, respectively, detected in 9.3% and 3.5% of small ruminants and in 7.4% and 0.2% of wild-living birds. Sheep had a significantly higher prevalence of C. jejuni and Salmonella than goats, but there were no differences due to season, location, or management. Pulsed field gel electrophoresis (PFGE) of isolated strains revealed geographic specificity and genomic diversity of both pathogens from small ruminants. However, C jejuni strains with indistinguishable PFGE profiles were isolated from one Rocic Dove and two European Starlings caught at separate locations. Matching C jejuni or Salmonella strain profiles were not found between small ruminants and wild-living birds. This study found that sheep pose a greater risk than goats in C jejuni and Salmonella contamination at co-grazing small ruminant farms. Wild-living birds also are potential carriers of C jejuni and Salmonella although no evidence of cross-contamination with small ruminants was established. (C) 2013 Elsevier B.V. All rights reserved. C1 [Pao, S.; Kim, C.; Wildeus, S.; Ren, S.; Long, W., III; Li, H.] Virginia State Univ, Agr Res Stn, Petersburg, VA 23806 USA. [Hagens, B. E.; Schwarz, J. G.] Univ Maryland Eastern Shore, Dept Agr, Princess Anne, MD USA. [Ettinger, M. R.] Virginia Dept Agr & Consumer Serv, Richmond, VA USA. [Wilson, M. D.; Watts, B. D.] Coll William & Mary, Ctr Conservat Biol, Williamsburg, VA USA. [Whitley, N. C.] North Carolina Agr & Tech State Univ, Greensboro, NC USA. [Porto-Fett, A. C. S.; Luchansky, J. B.] ARS, USDA, Wyndmoor, PA USA. [Kaseloo, R.] Virginia State Univ, Dept Biol, Petersburg, VA 23806 USA. RP Pao, S (reprint author), Virginia State Univ, Agr Res Stn, POB 9061, Petersburg, VA 23806 USA. EM spao@vsu.edu FU U.S. Department of Agriculture FX This article is a contribution of Virginia State University, Agricultural Research Station (Journal Article Series Number 311). Funding support from the U.S. Department of Agriculture for this study is acknowledged. Technical advice and/or assistance from Joni Collins, Sarah Weeda, and Catherine Baxley (VSU, Petersburg, VA), Mark Gooden and Harry Taylor (UMES, Princess Anne, MD), and Bradley Shoyer and Jeff Call (USDA-ARS, Wyndmoor, PA) are acknowledged. NR 49 TC 4 Z9 4 U1 1 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 EI 1878-0490 J9 LIVEST SCI JI Livest. Sci. PD FEB PY 2014 VL 160 BP 163 EP 171 DI 10.1016/j.livsci.2013.11.020 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA AA9KC UT WOS:000331411400020 ER PT J AU Kopittke, PM Wang, P Menzies, NW Naidu, R Kinraide, TB AF Kopittke, Peter M. Wang, Peng Menzies, Neal W. Naidu, Ravi Kinraide, Thomas B. TI A web-accessible computer program for calculating electrical potentials and ion activities at cell-membrane surfaces SO PLANT AND SOIL LA English DT Article DE Electrical potential; Mineral nutrition; Plant growth; Plant-ion interactions; Plasma membrane; Toxicity ID BIOTIC LIGAND MODEL; PLASMA-MEMBRANES; COWPEA SEEDLINGS; SALINE SOLUTIONS; WHEAT SEEDLINGS; ROOT ELONGATION; ZINC ABSORPTION; TOXICITY; CALCIUM; CATIONS AB Increasing evidence indicates that plant responses to ions (uptake/transport, inhibition, and alleviation of inhibition) are dependent upon ion activities at the outer surface of root-cell plasma membranes (PMs) rather than activities in the bulk-phase rooting medium. A web-accessible computer program was written to calculate the electrical potential (psi) at the outer surface of root-cell PMs (psi (PM)). From these values of psi (PM), activities of ion I with charge Z ({I (Z)}) can be calculated for the outer surface of the PM ({I (Z)}(PM)). In addition, psi and {I (Z)} in the Donnan phase of the cell walls (psi (CW) and {I (Z)}(CW)) can be calculated. By reanalysing published data, we illustrate how this computer program can assist in the investigation of plant-ion interactions. For example, we demonstrate that in saline solutions, both Ca deficiency and Na uptake are more closely related to {Ca2+}(PM) and {Na+}(PM) than to {Ca2+}(b) and {Na+}(b) (activities in the bulk-phase media). Additional examples are given for Zn and P nutrition, Ni toxicity, and arsenate uptake. The computer program presented here should assist others to develop an electrostatic view of plant-ion interactions and to re-evaluate some commonly-held views regarding mechanisms of ion transport, toxicity, competition among ions, and other phenomena. C1 [Kopittke, Peter M.; Wang, Peng; Menzies, Neal W.] Univ Queensland, Sch Agr & Food Sci, St Lucia, Qld 4072, Australia. [Kopittke, Peter M.; Menzies, Neal W.; Naidu, Ravi] CRC CARE, Salisbury South, SA 5106, Australia. [Naidu, Ravi] Univ S Australia, Ctr Environm Risk Assessment & Remediat, Mawson Lakes, SA 5095, Australia. [Kinraide, Thomas B.] ARS, USDA, Washington, DC USA. RP Kopittke, PM (reprint author), CRC CARE, POB 486, Salisbury South, SA 5106, Australia. EM p.kopittke@uq.edu.au; tom@kinraide.net RI Kopittke, Peter/A-6026-2011; Wang, Peng/E-2008-2012; Menzies, Neal/L-1338-2013 OI Kopittke, Peter/0000-0003-4948-1880; Wang, Peng/0000-0001-8622-8767; Menzies, Neal/0000-0003-0207-070X FU Australian Research Council (ARC) [FT120100277, DE130100943] FX Dr Kopittke is the recipient of an Australian Research Council (ARC) Future Fellowship (FT120100277). Dr Wang is a recipient of an ARC Discovery Early Career Researcher Award (DE130100943). NR 40 TC 9 Z9 9 U1 2 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD FEB PY 2014 VL 375 IS 1-2 BP 35 EP 46 DI 10.1007/s11104-013-1948-x PG 12 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA AA6JL UT WOS:000331204500003 ER PT J AU Scott, DA Burger, JA AF Scott, D. Andrew Burger, James A. TI Longleaf and loblolly pine seedlings respond differently to soil compaction, water content, and fertilization SO PLANT AND SOIL LA English DT Article DE Longleaf pine; Loblolly pine; Bulk density; Soil water content; Root length density ID FOREST SOILS; ROOT-GROWTH; NITROGEN; BIOMASS; CARBON; PRODUCTIVITY; AVAILABILITY; ALLOCATION; NUTRIENT AB Longleaf pine (Pinus palustris Mill.) is being restored across the U.S. South for a multitude of ecological and economic reasons, but our understanding of longleaf pine's response to soil physical conditions is poor. On the contrary, our understanding of loblolly pine (Pinus taeda L.) root and shoot growth response to soil conditions is well established. We performed a comparative greenhouse study which modeled root length density, total seedling biomass, and the ratio of aboveground:belowground mass as functions of volumetric water content, bulk density and soil fertility (fertilized or not). Root length density was about 35 % greater in longleaf pine seedlings compared to loblolly pine seedlings, and was reasonably well modeled (R (2) = 0.54) for longleaf pine by bulk density (linear), volumetric water content (quadratic), soil fertility, and the interactions of bulk density, volumetric water content, species, and soil fertility. The aboveground:belowground mass ratio (ABR) increased at both extremes of water content. This research indicates that young longleaf pine seedling root systems respond more negatively to extremes of soil physical conditions than loblolly pine, and compacted or dry loamy soils should be ameliorated in addition to normal competition control, especially on soils degraded by past management. C1 [Scott, D. Andrew] US Forest Serv, Southern Res Stn, USDA, Normal, AL 35762 USA. [Burger, James A.] Virginia Polytech Inst & State Univ, Dept Forestry, Blacksburg, VA 24061 USA. RP Scott, DA (reprint author), US Forest Serv, Southern Res Stn, USDA, POB 1927, Normal, AL 35762 USA. EM andyscott@fs.fed.us OI Scott, D. Andrew/0000-0002-2592-1522 NR 38 TC 1 Z9 1 U1 4 U2 32 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD FEB PY 2014 VL 375 IS 1-2 BP 255 EP 265 DI 10.1007/s11104-013-1929-0 PG 11 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA AA6JL UT WOS:000331204500020 ER PT J AU Pecora, A Malacari, DA Ridpath, JF Aguirreburualde, MSP Combessies, G Odeon, AC Romera, SA Golemba, MD Wigdorovitz, A AF Pecora, A. Malacari, D. A. Ridpath, J. F. Perez Aguirreburualde, M. S. Combessies, G. Odeon, A. C. Romera, S. A. Golemba, M. D. Wigdorovitz, A. TI First finding of genetic and antigenic diversity in 1b-BVDV isolates from Argentina SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Antigenic diversity; Bovine Viral Diarrhea Virus; Cattle viruses; Phylogenetic analysis; Pestivirus ID BOVINE-VIRAL DIARRHEA; CROSS-NEUTRALIZATION ASSAYS; SWINE-FEVER VIRUS; PHYLOGENETIC ANALYSIS; MUCOSAL DISEASE; MONOCLONAL-ANTIBODIES; E2 GLYCOPROTEIN; UNITED-STATES; PESTIVIRUSES; CATTLE AB Infection with Bovine Viral Diarrhea Viruses (BVDV) in cattle results in a wide range of clinical manifestations, ranging from mild respiratory disease to fetal death and mucosal disease, depending on the virulence of the virus and the immune and reproductive status of the host. In this study 30 Argentinean BVDV isolates were characterized by phylogenetic analysis. The isolates were genotyped based on comparison of the 5' untranslated region (5' UTR) and the E2 gene. In both phylogenetic trees, 76% of the viruses were assigned to BVDV 1b, whereas BVDV 1a, 2a and 2b were also found. Eight of the BVDV 1b isolates were further characterized by cross-neutralization tests using guinea pig antisera and sera from bovines vaccinated with two different commercial vaccines. The results demonstrated the presence of a marked antigenic diversity among Argentinean BVDV isolates and suggest the need to incorporate BVDV 1 isolates in diagnostic strategies. (C)13 Elsevier Ltd. All rights reserved. C1 [Pecora, A.; Malacari, D. A.; Perez Aguirreburualde, M. S.; Romera, S. A.; Wigdorovitz, A.] INTA Castelar, Inst Virol, Vet & Agr Sci Res Ctr, Buenos Aires, DF, Argentina. [Ridpath, J. F.] USDA, Ruminant Dis & Immunol Res Unit, Ames, IA USA. [Combessies, G.] Lab Azul Diagnost, Buenos Aires, DF, Argentina. [Odeon, A. C.] INTA Balcarce, Lab Sanidad Anim, Buenos Aires, DF, Argentina. [Golemba, M. D.] UBA, Fac Farm & Bioquim, Dept Virol, Buenos Aires, DF, Argentina. RP Malacari, DA (reprint author), Cabanas & Reseros S-N, RA-1712 Buenos Aires, DF, Argentina. EM dmalacari@cnia.inta.gov.ar OI Perez Aguirreburualde, Maria Sol/0000-0002-9599-1390 NR 59 TC 7 Z9 7 U1 1 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD FEB PY 2014 VL 96 IS 1 BP 204 EP 212 DI 10.1016/j.rvsc.2013.11.004 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA AA9OI UT WOS:000331422400032 PM 24295740 ER PT J AU Allison, AB Keel, MK Philips, JE Cartoceti, AN Munk, BA Nemeth, NM Welsh, TI Thomas, JM Crum, JM Lichtenwalner, AB Fadly, AM Zavala, G Holmes, EC Brown, JD AF Allison, Andrew B. Keel, M. Kevin Philips, Jamie E. Cartoceti, Andrew N. Munk, Brandon A. Nemeth, Nicole M. Welsh, Trista I. Thomas, Jesse M. Crum, James M. Lichtenwalner, Anne B. Fadly, Aly M. Zavala, Guillermo Holmes, Edward C. Brown, Justin D. TI Avian oncogenesis induced by lymphoproliferative disease virus: A neglected or emerging retroviral pathogen? SO VIROLOGY LA English DT Article DE Lymphoproliferative disease virus (LPDV); Alpharetrovirus; Avian tumor viruses; Exogenous retrovirus; Oncogenesis; Order Galliformes ID LONG TERMINAL REPEAT; WD-DOMAIN PROTEIN; C RETROVIRUS; PROMOTER INSERTION; TURKEYS; SEQUENCES; RETICULOENDOTHELIOSIS; INTEGRATION; LEUKOSIS; LPDV AB Lymphoproliferative disease virus (LPDV) is an exogenous oncogenic retrovirus that induces lymphoid tumors in some galliform species of birds. Historically, outbreaks of LPDV have been reported from Europe and Israel. Although the virus has previously never been detected in North America, herein we describe the widespread distribution, genetic diversity, pathogenesis, and evolution of LPDV in the United States. Characterization of the provirus genome of the index LPDV case from North America demonstrated an 88% nucleotide identity to the Israeli prototype strain. Although phylogenetic analysis indicated that the majority of viruses fell into a single North American lineage, a small subset of viruses from South Carolina were most closely related to the Israeli prototype. These results suggest that LPDV was transferred between continents to initiate outbreaks of disease. However, the direction (New World to Old World or vice versa), mechanism, and time frame of the transcontinental spread currently remain unknown. (C) 2013 Elsevier Inc. All rights reserved. C1 [Allison, Andrew B.] Cornell Univ, Coll Vet Med, Dept Microbiol & Immunol, Baker Inst Anim Hlth, Ithaca, NY 14853 USA. [Allison, Andrew B.; Keel, M. Kevin; Philips, Jamie E.; Cartoceti, Andrew N.; Munk, Brandon A.; Nemeth, Nicole M.; Welsh, Trista I.; Thomas, Jesse M.; Brown, Justin D.] Univ Georgia, Coll Vet Med, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Crum, James M.] West Virginia Div Nat Resources, Elkins, WV 26241 USA. [Lichtenwalner, Anne B.] Univ Maine, Dept Anim & Vet Sci, Hlth Anim Lab, Orono, ME 04469 USA. [Fadly, Aly M.] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Zavala, Guillermo] Univ Georgia, Poultry Diagnost Res Ctr, Dept Populat Hlth, Athens, GA 30602 USA. [Holmes, Edward C.] Univ Sydney, Sch Biol Sci, Marie Bashir Inst Infect Dis & Biosecur, Sydney, NSW 2006, Australia. [Holmes, Edward C.] Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia. RP Allison, AB (reprint author), Cornell Univ, Coll Vet Med, Dept Microbiol & Immunol, Baker Inst Anim Hlth, Ithaca, NY 14853 USA. EM aba75@cornell.edu OI Holmes, Edward/0000-0001-9596-3552 FU Pittman-Robertson Federal Aid in Wildlife Restoration; Arcadia Wildlife Preserve, Inc.; SCWDS; National Health and Medical Research Council Australia Fellowship; NRSA from the National Institute for Allergy and Infectious Diseases, National Institutes of Health [F32AI100545] FX We thank John Bryan, Elizabeth Bunting, Steven Kubiski, Joe Okoniewski, and Mark Ruder for diagnostic and field support, as well as other field biologists and veterinarians affiliated with the natural resource agency of each state for collection and submission of birds. Funding for this research was provided by Pittman-Robertson Federal Aid in Wildlife Restoration, the Arcadia Wildlife Preserve, Inc., and through the continued sponsorship of the SCWDS by member states and federal agencies. E.C.H. is supported by a National Health and Medical Research Council Australia Fellowship. Funding was additionally provided by a NRSA fellowship (F32AI100545) to A.B.A. from the National Institute for Allergy and Infectious Diseases, National Institutes of Health. NR 46 TC 3 Z9 3 U1 2 U2 9 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD FEB PY 2014 VL 450 BP 2 EP 12 DI 10.1016/j.viro1.2013.11.037 PG 11 WC Virology SC Virology GA AB0MC UT WOS:000331485500002 PM 24503062 ER PT J AU Edwards, MC Weiland, JJ AF Edwards, Michael C. Weiland, John J. TI Coat protein expression strategy of oat blue dwarf virus SO VIROLOGY LA English DT Article DE Marafivirus; Tymoviridae; OBDV ID MOSAIC-VIRUS; MESSENGER-RNA; SEQUENCE; GENE; TYMOVIRUSES; CLONE; CDNA; SITE AB Oat blue dwarf virus (OBDV) is a member of the genus Marafivirus whose genome encodes a 227 kDa polyprotein (p227) ostensibly processed post-translationally into its functional components. Encoded near the 3' terminus and coterminal with the p227 ORF are ORFs specifying major and minor capsid proteins (CP). Since the CP expression strategy of marafiviruses has not been thoroughly investigated, we produced a series of point mutants in the OBDV CP encoding gene and examined expression in protoplasts. Results support a model in which the 21 kDa major CP is the product of direct translation of a sgRNA, while the 24 kDa minor CP is a cleavage product derived from both the polyprotein and a larger similar to 26 kDa precursor translated directly from the sgRNA. Cleavage occurs at an LXG[G/A] motif conserved in many viruses that use papain-like proteases for polyprotein processing and protection against degradation via the ubiquitin-proteasome system. Published by Elsevier Inc. C1 [Edwards, Michael C.; Weiland, John J.] USDA ARS, Cereal Crops Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. RP Edwards, MC (reprint author), USDA ARS, Cereal Crops Res Unit, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM michael.edwards@ars.usda.gov FU USDA-ARS CRIS [5442-22000-048-00D] FX Funding for this work was provided by USDA-ARS CRIS project number 5442-22000-048-00D. NR 26 TC 3 Z9 3 U1 1 U2 6 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD FEB PY 2014 VL 450 BP 290 EP 296 DI 10.1016/j.virol.2013.12.018 PG 7 WC Virology SC Virology GA AB0MC UT WOS:000331485500032 PM 24503092 ER PT J AU Battisti, A Benvegnu, I Colombari, F Haack, RA AF Battisti, Andrea Benvegnu, Isadora Colombari, Fernanda Haack, Robert A. TI Invasion by the chestnut gall wasp in Italy causes significant yield loss in Castanea sativa nut production SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Cynipidae; Dryocosmus kuriphilus; gall; invasion; management; yield ID DRYOCOSMUS-KURIPHILUS YASUMATSU; CYNIPIDAE; HYMENOPTERA; BIOLOGY AB The Asian chestnut gall wasp Dryocosmus kuriphilusYasumatsu (Hymenoptera Cynipidae) is an invasive species in chestnut forests and orchards in many parts of the world. Nuts produced by the European chestnut (Castanea sativaMiller) are important in human food and culture, and as a component in food webs in forest ecosystems. Severe infestations are reported to reduce nut yield, although precise data are lacking because of large natural year-to-year variability in yield. The recent colonization of chestnut orchards in north-eastern Italy, where nut yield has been continuously and precisely recorded for several years, offered an opportunity to calculate the impact of gall wasp infestation level on yield. The nut yield of C.sativa chestnut trees was negatively related to the gall wasp infestation level, with losses as high as 80% being reported when the number of current-year galls was above six galls per 50-cm twig. Yield losses can be explained by direct and indirect factors related to gall formation, and a fuller understanding of the mechanisms involved could identify possible mitigation measures. C1 [Battisti, Andrea; Benvegnu, Isadora; Colombari, Fernanda] Univ Padua, Dept Agron Food Nat Resources Anim & Environm DAF, I-35020 Legnaro, Italy. [Benvegnu, Isadora] Veneto Agr, I-35020 Legnaro, Italy. [Haack, Robert A.] US Forest Serv, USDA, No Res Stn, E Lansing, MI 48823 USA. RP Battisti, A (reprint author), Univ Padua, Dept Agron Food Nat Resources Anim & Environm DAF, I-35020 Legnaro, Italy. EM andrea.battisti@unipd.it RI Battisti, Andrea/F-6243-2015 OI Battisti, Andrea/0000-0002-2497-3064 FU Regione del Veneto Servizi Fitosanitari FX The research was supported by a grant of Regione del Veneto Servizi Fitosanitari. We thank Giovanni Narduzzo of Treviso Forest Service and the chestnut growers association of Combai and Monfenera for their collaboration; Kurt Gottschalk, Giorgio Maresi, Lorenzo Marini and Toby Petrice for comments on an earlier version of the manuscript; and Greg Miller and Robert Stehli for sharing their personal experiences with the ACGW in Ohio. We also thank three anonymous reviewers for useful comments on early versions of the manuscript. NR 29 TC 12 Z9 13 U1 1 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD FEB PY 2014 VL 16 IS 1 BP 75 EP 79 DI 10.1111/afe.12036 PG 5 WC Entomology SC Entomology GA AA1LW UT WOS:000330858800008 ER PT J AU Kuhns, EH Tribuiani, Y Martini, X Meyer, WL Pena, J Hulcr, J Stelinski, LL AF Kuhns, Emily H. Tribuiani, Yolani Martini, Xavier Meyer, Wendy L. Pena, Jorge Hulcr, Jiri Stelinski, Lukasz L. TI Volatiles from the symbiotic fungus Raffaelea lauricola are synergistic with Manuka lures for increased capture of the Redbay ambrosia beetle Xyleborus glabratus SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Avocado; isoamyl acetate; isoamyl alcohol; laurel wilt; Persea americana; Persea borbonia; Scolytinae ID COLEOPTERA-CURCULIONIDAE SCOLYTINAE; SOUTHEASTERN UNITED-STATES; LAUREL WILT; PHOEBE OIL; LAURACEAE; FLORIDA; PATHOGEN; EFFICACY; DISEASE; VECTOR AB Redbay ambrosia beetle Xyleborus glabratus is an invasive wood boring beetle that has become established in the southeastern U.S.A. and transmits a fungus Raffaelea lauricola that causes lethal laurel wilt. Among susceptible Lauraceae hosts are redbay Persea borbonia and avocado Persea americana. There is a crucial need for detection of this pest as it moves into new areas. Consequently, our goal was to create a better lure for the monitoring and control of redbay ambrosia beetle. We analyzed volatile emissions of R. lauricola, created a synthetic odour blend based on this analysis and tested this odour blend as a potential attractant in a redbay forest infested with X. glabratus. The synthetic Raffaelea odour blend was not attractive to the beetles by itself. However, it synergistically increased attraction to host-mimic volatiles. We tested four commercial release devices for dispensing Raffaelea odour at various release rates. Two prototypes with the highest release rate, when paired with commercial manuka oil lures, captured more beetles than manuka oil lures alone. These results indicate that a synthetic blend of volatiles based on the odour of the symbiotic fungus of X. glabratus may be useful for the development of more sensitive monitoring lures for this invasive pathogen vector. C1 [Kuhns, Emily H.; Tribuiani, Yolani; Martini, Xavier; Meyer, Wendy L.; Stelinski, Lukasz L.] Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL 33850 USA. [Pena, Jorge] Univ Florida, Trop Res & Educ Ctr, Dept Entomol & Nematol, Homestead, FL 33031 USA. [Hulcr, Jiri] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Hulcr, Jiri] US Forest Serv, USDA, Asheville, NC 28801 USA. RP Stelinski, LL (reprint author), Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, 700 Expt Stn Rd, Lake Alfred, FL 33850 USA. EM stelinski@ufl.edu RI Martini, Xavier/E-7709-2012; OI Martini, Xavier/0000-0002-0556-3465 FU USDA SCRI; USDA Forest Service, Forest Health Protection FX Funding for this research was provided by a USDA SCRI grant. J. H. was funded by a cooperative agreement from the USDA Forest Service, Forest Health Protection. We thank members of the Stelinski Laboratory for their assistance with the field experiments. We thank Jack Smoot and Russell Rouseff for their assistance with the GC-MS. Alpha Scents, Inc., graciously provided prototype devices for field trials. NR 21 TC 13 Z9 13 U1 3 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD FEB PY 2014 VL 16 IS 1 BP 87 EP 94 DI 10.1111/afe.12037 PG 8 WC Entomology SC Entomology GA AA1LW UT WOS:000330858800010 ER PT J AU Burner, DM Brauer, DK Snider, JL Harrington, CA Moore, PA AF Burner, D. M. Brauer, D. K. Snider, J. L. Harrington, C. A. Moore, P. A., Jr. TI Phenological responses of juvenile pecan and white oak on an upland site SO AGROFORESTRY SYSTEMS LA English DT Article DE Budburst; Carya illinoiensis; Growth; Leaf senescence; Nitrogen fertilization; Quercus alba ID DOUGLAS-FIR; LEAF SENESCENCE; RADIAL GROWTH; TREES; PERFORMANCE; OREGON; WINTER AB Pecan (Carya illinoiensis) and white oak (Quercus alba) produce multiple products and wildlife values, but their phenological responses to N fertilization have not been well characterized. We compared tree growth at planting and for six consecutive growing seasons during establishment (2003-2008, Test 1), and determined if phenology of budburst, leaf area index (LAI), quantum yield of photosystem II (Fv/Fm), radial growth, and total chlorophyll concentration (a, b) responded to poultry litter fertilization supplying 0, 50, and 100 kg ha(-1) N (2010-2012, Test 2) in a mixed-species orchard on an upland site near Booneville, Arkansas. Species did not differ significantly in height in Test 1. Budburst was 9 days earlier for white oak than pecan in 2010. Budburst for both species could be predicted by accumulating chilling and forcing units throughout the dormant season. Maximum predicted radial growth was comparable for pecan (2.19 mm) and white oak (2.26 mm), and peaked 28 days earlier for white oak (3 June) than pecan (1 July). White oak LAI generally exceeded that of pecan during the growing season. Senescence began about 27 October regardless of species, and was better characterized by decreasing Fv/Fm or total chlorophyll concentration than LAI. Phenology was generally not responsive to N fertilization, perhaps because of adequate soil and foliar N. The study provides additional information on growth responses of these high-valued species to supplemental fertilization on an upland site. C1 [Burner, D. M.] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. [Brauer, D. K.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Snider, J. L.] Univ Georgia, Tifton, GA 31794 USA. [Harrington, C. A.] US Forest Serv, Pacific NW Res Stn, USDA, Olympia, WA 98512 USA. [Moore, P. A., Jr.] Univ Arkansas, Poultry Prod & Prod Safety Res Unit, USDA ARS, Fayetteville, AR 72701 USA. RP Snider, JL (reprint author), Univ Georgia, 115 Coastal Way, Tifton, GA 31794 USA. EM jlsnider@uga.edu FU USDA-ARS, Dale Bumpers Small Farms Research Center, Booneville, Arkansas [6227-21660-003-00D] FX USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. Drs. DM Burner, DK Brauer, and JL Snider were formerly located at the Dale Bumpers Small Farms Research Center, 6883 South State Hwy 23, Booneville, Arkansas, USA, 72927. Perdue AgriRecycle, Seaford, Delaware donated the Microstart60 poultry litter. The authors appreciated expert assistance provided by Dr. DE Brauer and technical expertise provided by K Chapman, T Horton, L Huddleston, J Whiley, and B Woolley. Principle funding for the research was provided by USDA-ARS, Dale Bumpers Small Farms Research Center, Booneville, Arkansas through Project 6227-21660-003-00D. NR 63 TC 3 Z9 3 U1 2 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4366 EI 1572-9680 J9 AGROFOREST SYST JI Agrofor. Syst. PD FEB PY 2014 VL 88 IS 1 BP 141 EP 155 DI 10.1007/s10457-013-9662-5 PG 15 WC Agronomy; Forestry SC Agriculture; Forestry GA AA0FW UT WOS:000330772000011 ER PT J AU Lackey, DE Burk, DH Ali, MR Mostaedi, R Smith, WH Park, J Scherer, PE Seay, SA McCoin, CS Bonaldo, P Adams, SH AF Lackey, Denise E. Burk, David H. Ali, Mohamed R. Mostaedi, Rouzbeh Smith, William H. Park, Jiyoung Scherer, Philipp E. Seay, Shundra A. McCoin, Colin S. Bonaldo, Paolo Adams, Sean H. TI Contributions of adipose tissue architectural and tensile properties toward defining healthy and unhealthy obesity SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE matrix metalloproteinase; bariatric surgery; adipose inflammation; type 2 diabetes mellitus; extracellular matrix ID NUTRITIONALLY INDUCED OBESITY; DIET-INDUCED OBESITY; INSULIN-RESISTANCE; EXTRACELLULAR-MATRIX; METABOLIC SYNDROME; CATHEPSIN-S; MACROPHAGE ACTIVATION; COLLAGEN VI; WEIGHT-LOSS; MICE AB The extracellular matrix (ECM) plays an important role in the maintenance of white adipose tissue (WAT) architecture and function, and proper ECM remodeling is critical to support WAT malleability to accomodate changes in energy storage needs. Obesity and adipocyte hypertrophy place a strain on the ECM remodeling machinery, which may promote disordered ECM and altered tissue integrity and could promote proinflammatory and cell stress signals. To explore these questions, new methods were developed to quantify omental and subcutaneous WAT tensile strength and WAT collagen content by three-dimensional confocal imaging, using collagen VI knockout mice as a methods validation tool. These methods, combined with comprehensive measurement of WAT ECM proteolytic enzymes, transcript, and blood analyte analyses, were used to identify unique pathophenotypes of metabolic syndrome and type 2 diabetes mellitus in obese women, using multivariate statistical modeling and univariate comparisons with weight-matched healthy obese individuals. In addition to the expected differences in inflammation and glycemic control, approximately 20 ECM-related factors, including omental tensile strength, collagen, and enzyme transcripts, helped discriminate metabolically compromised obesity. This is consistent with the hypothesis that WAT ECM physiology is intimately linked to metabolic health in obese humans, and the studies provide new tools to explore this relationship. C1 [Lackey, Denise E.; Seay, Shundra A.; Adams, Sean H.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Burk, David H.] Pennington Biomed Res Ctr, Cell Biol & Bioimaging Core, Baton Rouge, LA 70808 USA. [Ali, Mohamed R.; Mostaedi, Rouzbeh; Smith, William H.] Univ Calif Davis, Sch Med, Dept Surg, Davis, CA 95616 USA. [Park, Jiyoung; Scherer, Philipp E.] Univ Texas SW Med Ctr Dallas, Touchstone Diabet Ctr, Dallas, TX 75390 USA. [McCoin, Colin S.; Adams, Sean H.] Univ Calif Davis, Mol Cellular & Integrat Physiol Grad Grp, Davis, CA 95616 USA. [Bonaldo, Paolo] Univ Padua, Dept Histol Microbiol & Med Biotechnol, Padua, Italy. [Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. RP Adams, SH (reprint author), USDA ARS, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM sean.h.adams@ars.usda.gov OI McCoin, Colin/0000-0002-8557-6441 FU US Department of Agriculture-Agricultural Research Service (USDA-ARS) [5306-51530-019-00]; USDA-ARS Headquarters Postdoctoral Award; National Institute of Diabetes and Digestive and Kidney Diseases [R01-DK-078328]; NIH [R01-DK-55758, R01-CA-112023]; Department of Defense (USAMRMC) [BC085909]; COBRE [8P20-GM-103528]; NORC [2P30-DK-072476]; NIH FX This research was supported by US Department of Agriculture-Agricultural Research Service (USDA-ARS) Intramural Project No. 5306-51530-019-00, a USDA-ARS Headquarters Postdoctoral Award (D.E. Lackey), National Institute of Diabetes and Digestive and Kidney Diseases Grant R01-DK-078328 (to S. H. Adams), and NIH Grants R01-DK-55758 and R01-CA-112023 (to P. E. Scherer). J. Park was supported in part by a fellowship from the Department of Defense (USAMRMC BC085909). This work utilized the facilities of the Pennington Biomedical Research Center Cell Biology and Bioimaging Core, which are supported in part by COBRE (8P20-GM-103528) and NORC (2P30-DK-072476) center grants from the NIH. The USDA is an equal opportunity provider and employer. NR 60 TC 24 Z9 24 U1 1 U2 5 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 EI 1522-1555 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD FEB PY 2014 VL 306 IS 3 BP E233 EP E246 DI 10.1152/ajpendo.00476.2013 PG 14 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA AA4LU UT WOS:000331067900001 PM 24302007 ER PT J AU Murphy, AF Rondon, SI Marchosky, R Buchman, J Munyaneza, J AF Murphy, Alexzandra F. Rondon, Silvia I. Marchosky, Ruben Buchman, Jeremy Munyaneza, Joseph TI Evaluation of Beet Leafhopper Transmitted Virescence Agent Damage in the Columbia Basin SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Economic thresholds; Purple top disease; Integrated pest management; Phytoplasma; Circulifer ID PURPLE TOP PHYTOPLASMA; POPULATION-DYNAMICS; ASTER YELLOWS; POTATO; CICADELLIDAE; WASHINGTON; HEMIPTERA; OREGON; TRANSMISSION; DISEASE AB Potato purple top disease is caused by a phytoplasma known as Beet Leafhopper Transmitted Virescence Agent (BLTVA), which is vectored by the beet leafhopper (BLH, Circulifer tenellus Baker). Previous studies determined that BLTVA can cause significant reductions in yield and tuber quality; however, quantifying the damage caused by BLTVA and the insect vector has been challenging. In 2009-2011, potato plants at different growth stages were exposed to varying densities of BLH in a screen house located at the Hermiston Agricultural Research and Extension Center in Hermiston, OR. The densities of potentially infective BLH were one BLH per plant (low), two BLH per plant (medium), and five BLH per plant (high). Releases occurred at the following growth stages: vegetative, tuber initiation, tuber bulking, and maturation. The treatments were arranged in a randomized complete block design with three replications per treatment. Disease incidence was monitored weekly and yield was assessed. When all 3 years were combined, we found that increasing rates of disease incidence correlated with decreasing yields. We also found that greater yield losses were observed with later BLH release times. With both correlations, differences between years were a strong contributing factor. There was a mean decrease in yield of 0-12 % at a density of one BLH per plant, 6-19 % at two BLH per plant, and 6-20 % for five BLH per plant. These general trends in yield loss suggest that economically relevant damage may occur at levels as low as one or two potentially infective BLH per plant in the Columbia Basin. C1 [Murphy, Alexzandra F.; Rondon, Silvia I.; Marchosky, Ruben] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Dept Crop & Soil Sci, Hermiston, OR 97838 USA. [Buchman, Jeremy] Frito Lay Agr Res, Rhinelander, WI 54501 USA. [Munyaneza, Joseph] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Rondon, Silvia I.] Oregon State Univ, Hermiston Ag Res & Ext Ctr, Hermiston, OR 97838 USA. RP Rondon, SI (reprint author), Oregon State Univ, Hermiston Ag Res & Ext Ctr, 2121 S First St, Hermiston, OR 97838 USA. EM Silvia.Rondon@oregonstate.edu FU Oregon State Potato Commission; Washington State Potato Commission FX We would like to acknowledge Dr. Jim Crosslin (USDA-ARS, Prosser, WA) and Aymeric Goyer (OSU-HAREC) for providing assistance with initial analysis of leaf samples for BLTVA. We are grateful to Amanda Smith, Tanner Keys, Mary Adams, Jonathan Macias, Kortney Sweek, and Sneha Callah for providing technical assistance in sample collection and analysis. We would also like to gratefully acknowledge funding from the Oregon and Washington State Potato Commissions. We appreciate the contributions from multiple anonymous reviewers. NR 33 TC 3 Z9 3 U1 3 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2014 VL 91 IS 1 BP 101 EP 108 DI 10.1007/s12230-013-9335-y PG 8 WC Agronomy SC Agriculture GA AA2WR UT WOS:000330955400005 ER PT J AU Marvelli, RA Hobbs, HA Li, SX McCoppin, NK Domier, LL Hartman, GL Eastburn, DM AF Marvelli, Rita A. Hobbs, Houston A. Li, Shuxian McCoppin, Nancy K. Domier, Leslie L. Hartman, Glen L. Eastburn, Darin M. TI Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence SO ARCHIVES OF VIROLOGY LA English DT Article ID PLANT-PATHOGENIC FUNGUS; SUDDEN-DEATH SYNDROME; F-SP ROBINIAE; BIOLOGICAL-CONTROL; CHESTNUT BLIGHT; GLYCINE-MAX; GRAMINEARUM; VIRUSES; SOLANI; HYPOVIRULENCE AB Virulence and double-stranded RNA (dsRNA) profiles of 44 isolates of Fusarium virguliforme were compared. When grouped according to dsRNA profiles, isolates with large dsRNAs were significantly (Pa parts per thousand currency sign0.05) less virulent than isolates without dsRNAs. High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. The new viruses were most closely related to a group of unclassified viruses that included viruses of F. graminearum and Phlebiopsis gigantea and are related to members of the family Totiviridae. C1 [Marvelli, Rita A.; Hobbs, Houston A.; Eastburn, Darin M.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Li, Shuxian] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [McCoppin, Nancy K.; Domier, Leslie L.; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA. RP Domier, LL (reprint author), Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA. EM lisle.domier@ars.usda.gov FU USDA [NC-125]; Illinois Soybean Association; University of Illinois at Urbana Champaign Campus Research Board FX We thank A. M. Dorrance, W. L. Pedersen, and X. B. Yang for providing F. virguliforme isolates, J. Chee-Sanford and K. N. Lambert for use of gel imaging systems, W. Chen, C. J. D'Arcy, and K. N. Paige for their research advice, and W. A. Maffit for assistance in total RNA purifications. Funding for this project was provided, in part, from the USDA NC-125 project, The Illinois Soybean Association, and from the University of Illinois at Urbana Champaign Campus Research Board. NR 26 TC 10 Z9 11 U1 3 U2 28 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD FEB PY 2014 VL 159 IS 2 BP 349 EP 352 DI 10.1007/s00705-013-1760-1 PG 4 WC Virology SC Virology GA AA2YN UT WOS:000330960200019 PM 24009061 ER PT J AU Li, RG Fei, ZJ Ling, KS AF Li, Rugang Fei, Zhangjun Ling, Kai-Shu TI Molecular and biological properties of tomato necrotic stunt virus and development of a sensitive real-time RT-PCR assay SO ARCHIVES OF VIROLOGY LA English DT Article ID FAMILY POTYVIRIDAE; SMALL RNAS; DISCOVERY; SEQUENCE; APHIDS AB The newly identified tomato necrotic stunt virus (ToNStV), with a genome of 10,057 nucleotides, had 60 % nucleotide sequence and 70 % coat protein amino acid sequence identity to other potyviruses. Analysis of a second ToNStV isolate revealed two single nucleotide substitutions. ToNStV induced stunting and chlorotic to necrotic leaf symptoms on tomato plants. In host-range assays, ToNStV systemically infected many species in the family Solanaceae and locally infected some species in the families Amaranthaceae, Brassicaceae and Cucurbitaceae. A sensitive and reliable real-time RT-PCR assay was developed using simply prepared crude tissue extract. C1 [Li, Rugang; Ling, Kai-Shu] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Ling, KS (reprint author), USDA ARS, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM Rugang.Li@ars.usda.gov; zf25@cornell.edu; kai.ling@ars.usda.gov OI Li, Rugang/0000-0001-6514-496X FU USDA, National Institute of Food and Agriculture's Specialty Crop Research Initiative program [SCRI 2010-600-25320] FX This work was supported in part by USDA, National Institute of Food and Agriculture's Specialty Crop Research Initiative program (SCRI 2010-600-25320) to KSL. We thank Dr. Alvin Simmons for his critical reading of the manuscript, and Andrea Gilliard and Emily Fillippeli for their excellent technical assistance. We appreciate the collaborating greenhouse tomato growers in North America and HM Clause Seed Company for sample collection in various locations. NR 19 TC 4 Z9 4 U1 1 U2 8 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD FEB PY 2014 VL 159 IS 2 BP 353 EP 358 DI 10.1007/s00705-013-1830-4 PG 6 WC Virology SC Virology GA AA2YN UT WOS:000330960200020 PM 24013235 ER PT J AU Hidalgo, B Irvin, MR Sha, J Zhi, DG Aslibekyan, S Absher, D Tiwari, HK Kabagambe, EK Ordovas, JM Arnett, DK AF Hidalgo, Bertha Irvin, M. Ryan Sha, Jin Zhi, Degui Aslibekyan, Stella Absher, Devin Tiwari, Hemant K. Kabagambe, Edmond K. Ordovas, Jose M. Arnett, Donna K. TI Epigenome-Wide Association Study of Fasting Measures of Glucose, Insulin, and HOMA-IR in the Genetics of Lipid Lowering Drugs and Diet Network Study SO DIABETES LA English DT Article ID BETA-CELL FUNCTION; TYPE-2 DIABETES-MELLITUS; BODY-MASS INDEX; DNA METHYLATION; FENOFIBRATE TREATMENT; MISSING HERITABILITY; COMPLEX DISEASES; HOMEOSTASIS; EXPRESSION; RESISTANCE AB Known genetic susceptibility loci for type 2 diabetes (T2D) explain only a small proportion of heritable T2D risk. We hypothesize that DNA methylation patterns may contribute to variation in diabetes-related risk factors, and this epigenetic variation across the genome can contribute to the missing heritability in T2D and related metabolic traits. We conducted an epigenome-wide association study for fasting glucose, insulin, and homeostasis model assessment of insulin resistance (HOMA-IR) among 837 nondiabetic participants in the Genetics of Lipid Lowering Drugs and Diet Network study, divided into discovery (N = 544) and replication (N = 293) stages. Cytosine guanine dinucleotide (CpG) methylation at approximate to 470,000 CpG sites was assayed in CD4(+) T cells using the Illumina Infinium HumanMethylation 450 Beadchip. We fit a mixed model with the methylation status of each CpG as the dependent variable, adjusting for age, sex, study site, and T-cell purity as fixed-effects and family structure as a random-effect. A Bonferroni corrected P value of 1.1 x 10(-7) was considered significant in the discovery stage. Significant associations were tested in the replication stage using identical models. Methylation of a CpG site in ABCG1 on chromosome 21 was significantly associated with insulin (P = 1.83 x 10(-7)) and HOMA-IR (P = 1.60 x 10(-9)). Another site in the same gene was significant for HOMA-IR and of borderline significance for insulin (P = 1.29 x 10(-7) and P = 3.36 x 10(-6), respectively). Associations with the top two signals replicated for insulin and HOMA-IR (P = 5.75 x 10(-3) and P = 3.35 x 10(-2), respectively). Our findings suggest that methylation of a CpG site within ABCG1 is associated with fasting insulin and merits further evaluation as a novel disease risk marker. C1 [Hidalgo, Bertha; Zhi, Degui; Tiwari, Hemant K.] Univ Alabama Birmingham, Dept Biostat, Sect Stat Genet, Birmingham, AL 35294 USA. [Irvin, M. Ryan; Sha, Jin; Aslibekyan, Stella; Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Absher, Devin] Hudson Alpha Inst Biotechnol, Huntsville, AL USA. [Kabagambe, Edmond K.] Vanderbilt Univ, Dept Med, Nashville, TN USA. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Medford, MA 02155 USA. RP Hidalgo, B (reprint author), Univ Alabama Birmingham, Dept Biostat, Sect Stat Genet, Birmingham, AL 35294 USA. EM bhidalgo@uab.edu FU National Heart, Lung, and Blood Institute [U01-HL072524-04]; National Heart, Lung, and Blood Institute, University of Alabama at Birmingham Statistical Genetics Postdoctoral Training Program [5T32-HL072757] FX This work was funded by National Heart, Lung, and Blood Institute grant U01-HL072524-04. B.H. was also funded by the National Heart, Lung, and Blood Institute, University of Alabama at Birmingham Statistical Genetics Postdoctoral Training Program, Grant 5T32-HL072757. NR 33 TC 44 Z9 45 U1 5 U2 17 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA SN 0012-1797 EI 1939-327X J9 DIABETES JI Diabetes PD FEB PY 2014 VL 63 IS 2 BP 801 EP 807 DI 10.2337/db13-1100 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA AA5BG UT WOS:000331110000045 PM 24170695 ER PT J AU Taylor, DL Hollingsworth, TN McFarland, JW Lennon, NJ Nusbaum, C Ruess, RW AF Taylor, D. Lee Hollingsworth, Teresa N. McFarland, Jack W. Lennon, Niall J. Nusbaum, Chad Ruess, Roger W. TI A first comprehensive census of fungi in soil reveals both hyperdiversity and fine-scale niche partitioning SO ECOLOGICAL MONOGRAPHS LA English DT Article DE biodiversity; black spruce forest; community assembly; fungi; fungus-to-plant ratio; global species richness; interior Alaska; USA; Picea mariana; rarefaction; ribosomal internal transcribed spacer; soil horizon; vegetation structure ID INTERNAL TRANSCRIBED SPACER; MULTIPLE SEQUENCE ALIGNMENT; BOREAL FOREST; COMMUNITY STRUCTURE; SPECIES RICHNESS; SPOROCARP DNA; ECTOMYCORRHIZAL FUNGI; MICROBIAL DIVERSITY; SYMBIOTIC FUNGI; HIGH-THROUGHPUT AB Fungi play key roles in ecosystems as mutualists, pathogens, and decomposers. Current estimates of global species richness are highly uncertain, and the importance of stochastic vs. deterministic forces in the assembly of fungal communities is unknown. Molecular studies have so far failed to reach saturated, comprehensive estimates of fungal diversity. To obtain a more accurate estimate of global fungal diversity, we used a direct molecular approach to census diversity in a boreal ecosystem with precisely known plant diversity, and we carefully evaluated adequacy of sampling and accuracy of species delineation. We achieved the first exhaustive enumeration of fungi in soil, recording 1002 taxa in this system. We show that the fungus:plant ratio in Picea mariana forest soils from interior Alaska is at least 17:1 and is regionally stable. A global extrapolation of this ratio would suggest 6 million species of fungi, as opposed to leading estimates ranging from 616000 to 1.5 million. We also find that closely related fungi often occupy divergent niches. This pattern is seen in fungi spanning all major functional guilds and four phyla, suggesting a major role of deterministic niche partitioning in community assembly. Extinctions and range shifts are reorganizing biodiversity on Earth, yet our results suggest that 98% of fungi remain undescribed and that many of these species occupy unique niches. C1 [Taylor, D. Lee; McFarland, Jack W.; Ruess, Roger W.] Univ Alaska, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Hollingsworth, Teresa N.] US Forest Serv, USDA, PNW Res Stn, Boreal Ecol Cooperat Res Unit, Fairbanks, AK 99775 USA. [Lennon, Niall J.; Nusbaum, Chad] Broad Inst MIT & Harvard, Cambridge, MA 02141 USA. RP Taylor, DL (reprint author), Univ New Mexico, Dept Biol, MSC03 2020,1, Albuquerque, NM 87131 USA. EM fflt@unm.edu OI Taylor, Donald/0000-0002-5985-9210 FU National Science Foundation [EF-0333308, ARC-0632332, DEB-0620579]; USDA Forest Service, Pacific Northwest Research Station [RJVA-PNW-01-JV-11261952-231] FX We thank Jozsef Geml, Ian Herriott, and Ina Timling for technical assistance, and Sarah Youssof, Meredith Blackwell, and John Taylor for helpful comments on the manuscript. Lisa Green and Scott Anderson assisted with sequencing at Broad. Shawn Houston, and the UAF Life Sciences Informatics core provided critical computational support. This study was supported by the National Science Foundation through awards EF-0333308, ARC-0632332, and DEB-0620579 and by the USDA Forest Service, Pacific Northwest Research Station, through agreement number RJVA-PNW-01-JV-11261952-231. This paper is dedicated to the memory of Michael G. Booth, an inspiring scientist and friend. NR 104 TC 65 Z9 65 U1 11 U2 114 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9615 EI 1557-7015 J9 ECOL MONOGR JI Ecol. Monogr. PD FEB PY 2014 VL 84 IS 1 BP 3 EP 20 DI 10.1890/12-1693.1 PG 18 WC Ecology SC Environmental Sciences & Ecology GA AA6NT UT WOS:000331215700002 ER PT J AU Li, N Xiao, LH Alderisio, K Elwin, K Cebelinski, E Chalmers, R Santin, M Fayer, R Kvac, M Ryan, U Sak, B Stanko, M Guo, YQ Wang, L Zhang, LX Cai, JZ Roellig, D Feng, YY AF Li, Na Xiao, Lihua Alderisio, Keri Elwin, Kristin Cebelinski, Elizabeth Chalmers, Rachel Santin, Monica Fayer, Ronald Kvac, Martin Ryan, Una Sak, Bohumil Stanko, Michal Guo, Yaqiong Wang, Lin Zhang, Longxian Cai, Jinzhong Roellig, Dawn Feng, Yaoyu TI Subtyping Cryptosporidium ubiquitum, a Zoonotic Pathogen Emerging in Humans SO EMERGING INFECTIOUS DISEASES LA English DT Article ID NEW-YORK; CERVINE GENOTYPE; SOURCE TRACKING; SMALL MAMMALS; SPP.; IDENTIFICATION; GLYCOPROTEIN; CHILDREN; PARVUM; TRANSMISSION AB Cryptosporidium ubiquitum is an emerging zoonotic pathogen. In the past, it was not possible to identify an association between cases of human and animal infection. We conducted a genomic survey of the species, developed a subtyping tool targeting the 60-kDa glycoprotein (gp60) gene, and identified 6 subtype families (XIIa-XIIf) of C. ubiquitum. Host adaptation was apparent at the gp60 locus; subtype XIIa was found in ruminants worldwide, subtype families XIIb-XIId were found in rodents in the United States, and XIIe and XIIf were found in rodents in the Slovak Republic. Humans in the United States were infected with isolates of subtypes XIIb-XIId, whereas those in other areas were infected primarily with subtype XIIa isolates. In addition, subtype families XIIb and XIId were detected in drinking source water in the United States. Contact with C. ubiquitum-infected sheep and drinking water contaminated by infected wildlife could be sources of human infections. C1 [Li, Na; Guo, Yaqiong; Wang, Lin; Feng, Yaoyu] E China Univ Sci & Technol, Shanghai 200237, Peoples R China. [Li, Na; Xiao, Lihua; Guo, Yaqiong; Roellig, Dawn] Ctr Dis Control & Prevent, Atlanta, GA USA. [Alderisio, Keri] New York City Dept Environm Protect, Flushing, NY USA. [Elwin, Kristin; Chalmers, Rachel] UK Cryptosporidium Reference Unit, Swansea, W Glam, Wales. [Cebelinski, Elizabeth] Minnesota Dept Hlth, St Paul, MN USA. [Santin, Monica; Fayer, Ronald] USDA, Beltsville, MD 20705 USA. [Kvac, Martin; Sak, Bohumil] Acad Sci Czech Republic, Ceske Budejovice, Czech Republic. [Ryan, Una] Murdoch Univ, Perth, WA, Australia. [Stanko, Michal] Slovak Acad Sci, Kosice, Slovakia. [Zhang, Longxian] Henan Agr Univ, Zhengzhou, Peoples R China. [Cai, Jinzhong] Qinghai Acad Vet Med & Anim Sci, Xining, Peoples R China. RP Feng, YY (reprint author), E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China. EM yyfeng@ecust.edu.cn RI Xiao, Lihua/B-1704-2013; Feng, Yaoyu/B-3076-2014; Kvac, Martin/G-7299-2014; Sak, Bohumil/G-9262-2014; Yaqiong, Guo/N-4927-2015 OI Xiao, Lihua/0000-0001-8532-2727; Kvac, Martin/0000-0003-0013-6090; FU National Natural Science Foundation of China [31229005, 31110103901, 31302078]; State Key Laboratory of Veterinary Etiological Biology, Lanzhou, China; Fundamental Research Funds for the Central Universities, China; Ministry of Education, Youth and Sports of the Czech Republic [LH11061] FX This study was supported in part by the National Natural Science Foundation of China (nos. 31229005, 31110103901, and 31302078); Open Funding Project of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou, China; Fundamental Research Funds for the Central Universities, China; and the Ministry of Education, Youth and Sports of the Czech Republic (no. LH11061). NR 29 TC 37 Z9 38 U1 4 U2 23 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD FEB PY 2014 VL 20 IS 2 BP 217 EP 224 DI 10.3201/eid2002.121797 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 301TA UT WOS:000330553800006 PM 24447504 ER PT J AU Griffith, J Blaney, D Shadomy, S Lehman, M Pesik, N Tostenson, S Delaney, L Tiller, R DeVries, A Gomez, T Sullivan, M Blackmore, C Stanek, D Lynfield, R AF Griffith, Jayne Blaney, David Shadomy, Sean Lehman, Mark Pesik, Nicki Tostenson, Samantha Delaney, Lisa Tiller, Rebekah DeVries, Aaron Gomez, Thomas Sullivan, Maureen Blackmore, Carina Stanek, Danielle Lynfield, Ruth CA Anthrax Invest Team TI Investigation of Inhalation Anthrax Case, United States SO EMERGING INFECTIOUS DISEASES LA English DT Article ID BACILLUS-ANTHRACIS AB Inhalation anthrax occurred in a man who vacationed in 4 US states where anthrax is enzootic. Despite an extensive multi-agency investigation, the specific source was not detected, and no additional related human or animal cases were found. Although rare, inhalation anthrax can occur naturally in the United States. C1 [Griffith, Jayne; Tostenson, Samantha; DeVries, Aaron; Sullivan, Maureen; Lynfield, Ruth] Minnesota Dept Hlth, St Paul, MN 55164 USA. [Blaney, David; Shadomy, Sean; Lehman, Mark; Pesik, Nicki; Delaney, Lisa; Tiller, Rebekah] Ctr Dis Control & Prevent, Atlanta, GA USA. [Gomez, Thomas] USDA, Atlanta, GA USA. [Blackmore, Carina; Stanek, Danielle] Florida Dept Hlth, Tallahassee, FL USA. RP Griffith, J (reprint author), Minnesota Dept Hlth, 625 Robert St North,POB 64975, St Paul, MN 55164 USA. EM jayne.griffith@state.mn.us NR 15 TC 6 Z9 6 U1 0 U2 4 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD FEB PY 2014 VL 20 IS 2 BP 280 EP 283 DI 10.3201/eid2002.130021 PG 4 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 301TA UT WOS:000330553800017 PM 24447835 ER PT J AU Martinez, G Pachepsky, YA Whelan, G Yakirevich, AM Guber, A Gish, TJ AF Martinez, Gonzalo Pachepsky, Yakov A. Whelan, Gene Yakirevich, Alexander M. Guber, Andrey Gish, Timothy J. TI Rainfall-induced fecal indicator organisms transport from manured fields: Model sensitivity analysis SO ENVIRONMENT INTERNATIONAL LA English DT Article DE Fecal indicator organisms; Release and transport; Global sensitivity analysis; Regression trees ID GRASS BUFFER CONDITIONS; ESCHERICHIA-COLI; PARAMETER UNCERTAINTY; WATER-QUALITY; RELEASE; RUNOFF; FATE; CRYPTOSPORIDIUM; MANAGEMENT; PATHOGENS AB Microbial quality of surface waters attracts attention due to food- and waterborne disease outbreaks. Fecal indicator organisms (FIOs) are commonly used for the microbial pollution level evaluation. Models predicting the fate and transport of FIOs are required to design and evaluate best management practices that reduce the microbial pollution in ecosystems and water sources and thus help to predict the risk of food and waterborne diseases. In this study we performed a sensitivity analysis for the KINEROS/STWIR model developed to predict the FIOs transport out of manured fields to other fields and water bodies in order to identify input variables that control the transport uncertainty. The distributions of model input parameters were set to encompass values found from three-year experiments at the USDA-ARS OPE3 experimental site in Beltsville and publicly available information. Sobol' indices and complementary regression trees were used to perform the global sensitivity analysis of the model and to explore the interactions between model input parameters on the proportion of FIO removed from fields. Regression trees provided a useful visualization of the differences in sensitivity of the model output in different parts of the input variable domain. Environmental controls such as soil saturation, rainfall duration and rainfall intensity had the largest influence in the model behavior, whereas soil and manure properties ranked lower. The field length had only moderate effect on the model output sensitivity to the model inputs. Among the manure-related properties the parameter determining the shape of the FIO release kinetic curve had the largest influence on the removal of FIOs from the fields. That underscored the need to better characterize the FIO release kinetics. Since the most sensitive model inputs are available in soil and weather databases or can be obtained using soil water models, results indicate the opportunity of obtaining large-scale estimates of FIO transport from fields based on publicly available rather than site-specific information. Published by Elsevier Ltd C1 [Martinez, Gonzalo] Univ Cordoba, Dept Agron, E-14071 Cordoba, Spain. [Martinez, Gonzalo; Pachepsky, Yakov A.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Whelan, Gene] US EPA, Natl Exposure Res Lab, Ecosyst Res Div, Athens, GA USA. [Yakirevich, Alexander M.] Ben Gurion Univ Negev, Zuckerberg Inst Water Res, Jacob Blaustein Inst Desert Res, IL-84990 Sede Boqer, Israel. [Gish, Timothy J.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Guber, Andrey] Michigan State Univ, Dep Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Martinez, G (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RI Martinez Garcia, Gonzalo/E-3069-2013; OI Martinez Garcia, Gonzalo/0000-0001-5575-9397; Pachepsky, Yakov/0000-0003-0232-6090 FU Spanish Ministry of Education [EX-2009-0429]; United States Environmental Protection Agency through Office of Research and Development [DW-12-92348101] FX The authors would like to thank the valuable and useful contribution of two anonymous referees. The first author would like to thank the Spanish Ministry of Educationfor the grant EX-2009-0429. The United States Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here under contract DW-12-92348101 to the USDA-ARS. It has been subjected to Agency review and approved for publication. NR 53 TC 7 Z9 9 U1 0 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD FEB PY 2014 VL 63 BP 121 EP 129 DI 10.1016/j.envint.2013.11.003 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA AA2FG UT WOS:000330909900014 PM 24291764 ER PT J AU Sigua, GC Chase, CC Albano, J AF Sigua, Gilbert C. Chase, Chad C., Jr. Albano, Joseph TI Soil-extractable phosphorus and phosphorus saturation threshold in beef cattle pastures as affected by grazing management and forage type SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Beef cattle pastures; Degree of soil phosphorus saturation; Forage type; Phosphorus; Rotational grazing; Subtropical; Zero grazing ID SHORTGRASS STEPPE; WATER-QUALITY; NUTRIENT; NITROGEN; RUNOFF; OPERATIONS; DYNAMICS; RANGE; ECOSYSTEM; SORPTION AB Grazing can accelerate and alter the timing of nutrient transfer, and could increase the amount of extractable phosphorus (P) cycle from soils to plants. The effects of grazing management and/or forage type that control P cycling and distribution in pasture's resources have not been sufficiently evaluated. Our ability to estimate the levels and changes of soil-extractable P and other crop nutrients in subtropical beef cattle pastures has the potential to improve our understanding of P dynamics and nutrient cycling at the landscape level. To date, very little attention has been paid to evaluating transfers of extractable P in pasture with varying grazing management and different forage type. Whether or not P losses from grazed pastures are significantly greater than background losses and how these losses are affected by soil, forage management, or stocking density are not well understood. The objective of this study was to evaluate the effect of grazing management (rotational versus "zero" grazing) and forage types (FT; bahiagrass, Paspalum notatum, Flugge versus rhizoma peanuts, Arachis glabrata, Benth) on the levels of extractable soil P and degree of P saturation in beef cattle pastures. This study (2004-2007) was conducted at the Subtropical Agricultural Research Station, US Department of Agriculture-Agricultural Research Service located 7 miles north of Brooksville, FL. Soil (Candler fine sand) at this location was described as well-drained hyperthermic uncoated Typic Quartzipsamments. A split plot arrangement in a completely randomized block design was used and each treatment was replicated four times. The main plot was represented by grazing management (grazing vs. no grazing) while forage types (bahiagrass vs. perennial peanut) as the sub-plot treatment. Eight steel exclosures (10 x 10 m) were used in the study. Four exclosures were placed and established in four pastures with bahiagrass and four exclosures were established in four pastures with rhizoma peanuts to represent the "zero" grazing treatment. The levels of soil-extractable P and degree of P saturation (averaged across FT and soil depth) of 22.1 mg kg(-1) and 11.6 % in pastures with zero grazing were not significantly (p <= 0.05) different from the levels of soil-extractable P and degree of P saturation of 22.8 mg kg(-1) and 12.9 % in pastures with rotational grazing, respectively. On the effect of FT, levels of soil-extractable P and degree of P saturation were significantly higher in pastures with rhizoma peanuts than in pastures with bahiagrass. There was no net gain of soil-extractable P due to the presence of animals in pastures with rotational grazing. Averaged across years, soil-extractable P in pastures with rotational grazing and with "zero" grazing was less than 150 mg kg(-1), the water quality protection. There had been no movement of soil-extractable P into the soil pedon since average degree of P saturation in the upper 15 cm was 14.3 % while the average degree of P saturation in soils at 15-30 cm was about 9.9 %. Overall, average extractable P did not exceed the crop requirement threshold of 50 mg P kg(-1) and the soil P saturation threshold of 25 %, suggesting that reactive P is not a problem. Our study revealed that rhizoma peanuts and bahiagrass differ both in their capacity to acquire nutrients from the soil and in the amount of nutrients they need per unit growth. Rhizoma peanuts, which are leguminous forage, would require higher amounts of P compared with bahiagrass. The difference in the amount of P needed by these forages could have a profound effect on their P uptake that can be translated to the remaining amount of P in the soils. Periodic applications of additional P may be necessary especially for pastures with rhizoma peanuts to sustain their agronomic needs and to potentially offset the export of P due to animal production. Addition of organic amendments could represent an important strategy to protect pasture lands from excessive soil resources exploitation. C1 [Sigua, Gilbert C.] USDA ARS, Florence, SC 29501 USA. [Chase, Chad C., Jr.] USDA ARS, Clay Ctr, NE USA. [Albano, Joseph] USDA ARS, Ft Pierce, FL USA. RP Sigua, GC (reprint author), USDA ARS, Florence, SC 29501 USA. EM gilbert.sigua@ars.usda.gov NR 49 TC 1 Z9 1 U1 1 U2 38 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD FEB PY 2014 VL 21 IS 3 BP 1691 EP 1700 DI 10.1007/s11356-013-2050-x PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA AA3CL UT WOS:000330970400009 PM 23955167 ER PT J AU Allen, BL Engeman, RM Leung, LKP AF Allen, Benjamin L. Engeman, Richard M. Leung, Luke K-P. TI The short-term effects of a routine poisoning campaign on the movements and detectability of a social top-predator SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Behaviourally mediated trophic cascade; Canis lupus dingo; Detection probability; Mesopredator release; Passive tracking index; Poison baiting; Relative abundance indices; Sodium fluoroacetate or 1080 ID NORTH-WESTERN AUSTRALIA; HOME-RANGE ANALYSIS; MANIPULATION EXPERIMENTS; MESOPREDATOR RELEASE; BEHAVIORAL ECOLOGY; TROPHIC REGULATOR; ARID AUSTRALIA; LETHAL CONTROL; CANIS-LUPUS; DINGO FENCE AB Top-predators can be important components of resilient ecosystems, but they are still controlled in many places to mitigate a variety of economic, environmental and/or social impacts. Lethal control is often achieved through the broad-scale application of poisoned baits. Understanding the direct and indirect effects of such lethal control on subsequent movements and behaviour of survivors is an important pre-requisite for interpreting the efficacy and ecological outcomes of top-predator control. In this study, we use GPS tracking collars to investigate the fine-scale and short-term movements of dingoes (Canis lupus dingo and other wild dogs) in response to a routine poison-baiting program as an example of how a common, social top-predator can respond (behaviourally) to moderate levels of population reduction. We found no consistent control-induced differences in home range size or location, daily distance travelled, speed of travel, temporal activity patterns or road/trail usage for the seven surviving dingoes we monitored immediately before and after a typical lethal control event. These data suggest that the spatial behaviour of surviving dingoes was not altered in ways likely to affect their detectability, and if control-induced changes in dingoes' ecological function did occur, these may not be related to altered spatial behaviour or movement patterns. C1 [Allen, Benjamin L.; Leung, Luke K-P.] Univ Queensland, Sch Agr & Food Sci, Gatton, Qld 4343, Australia. [Allen, Benjamin L.] Univ Canberra, Invas Anim Cooperat Res Ctr, Bruce, ACT 2617, Australia. [Engeman, Richard M.] USDA, Natl Wildlife Res Ctr, Ft Collins, CO USA. RP Allen, BL (reprint author), Biosecur Queensland, Robert Wicks Pest Anim Res Ctr, Toowoomba, Qld 4350, Australia. EM benjamin.allen@daff.qld.gov.au OI Allen, Benjamin/0000-0002-1533-0163 FU Meat and Livestock Australia; Australian Wool Innovation; New South Wales Department of Primary Industries FX Greg Conners provided access to the field site. Joseph Allen assisted in the trapping of dingoes, and Simon Cobb and Stuart Boyd-Law assisted in collar recovery. Joe Scanlon, Lee Allen and Matt Gentle provided helpful comments on earlier drafts of the manuscript. The project was undertaken through the Invasive Animals Cooperative Research Centre with funds provided by Meat and Livestock Australia, Australian Wool Innovation and the New South Wales Department of Primary Industries. The study was conducted under approval from an animal ethics committee and the Australian Pesticides and Veterinary Medicines Authority. NR 78 TC 8 Z9 8 U1 1 U2 27 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD FEB PY 2014 VL 21 IS 3 BP 2178 EP 2190 DI 10.1007/s11356-013-2118-7 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA AA3CL UT WOS:000330970400053 PM 24043505 ER PT J AU Kearns, HSJ Jacobi, WR Reich, RM Flynn, RL Burns, KS Geils, BW AF Kearns, H. S. J. Jacobi, W. R. Reich, R. M. Flynn, R. L. Burns, K. S. Geils, B. W. TI Risk of white pine blister rust to limber pine in Colorado and Wyoming, USA SO FOREST PATHOLOGY LA English DT Article ID CRONARTIUM-RIBICOLA; BRITISH-COLUMBIA; NORTH-AMERICA; 1ST REPORT; MANAGEMENT; DISEASE; RIBES; FORESTS AB Cronartium ribicola, the introduced pathogen that causes white pine blister rust (WPBR), continues to spread to additional limber pine populations in the Southern Rocky Mountains of Colorado and Wyoming. Because WPBR can severely impact ecosystems, forecasts of its potential distribution and incidence would be useful to land managers. Site and climate data from long infested study areas in Wyoming were fit with two regression models [logistic and classification and regression trees (CART)] to determine the environmental conditions associated with the distribution of WPBR. These models were then used to map limber pine stands at risk of infestation by C.ribicola throughout Wyoming (where it has long occurred) and Colorado (where it is just becoming established). Although variables representing vegetation and landform could identify infested plots, 1-km-scale climate variables for monthly temperature and moisture were better predictors of current WPBR distribution and were available for mapping expected future distribution across the region. Of 280485ha where limber pine was projected to occur in Colorado, 41% was forecast by the logistic model to be at risk of infestation, and 53%, by the CART model. Of an estimated 782229ha in Wyoming with limber pine, the logistic model projected 61% to be at risk; CART projected 79%. Additional regression models were fit with site and climate data to predict WPBR incidence (per cent of trees infected) and intensification (incidence/age of the oldest canker). Nearly one half of the plot-to-plot variation in incidence was explained using environmental variables readily available to land managers. Although mean plot incidence increased over time, mean intensification decreased 50% per decade. This work provides managers with several tools to reduce uncertainty over the expected distribution and incidence of WPBR, but surveillance and monitoring remain prudent activities for supplementing forecasts of WPBR epidemics. C1 [Kearns, H. S. J.; Jacobi, W. R.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Kearns, H. S. J.] US Forest Serv, USDA, Sandy, OR USA. [Reich, R. M.] Colorado State Univ, Dept Forest Rangeland & Watershed Stewardship, Ft Collins, CO 80523 USA. [Flynn, R. L.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Burns, K. S.] US Forest Serv, USDA, Golden, CO USA. [Geils, B. W.] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ USA. RP Jacobi, WR (reprint author), Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. EM william.jacobi@colostate.edu FU USDA Forest Service Special Development and Technology Program [01-CS-11020000-088]; USDA Forest Service Rocky Mountain Research Station [03-JV-11221605-298]; Colorado Agricultural Experiment Station FX We appreciate the contributions of B. Bromelow, B. Goodrich, K. Mogab, M. Moss, C. Park, J. Watkins, W. Romme and J. zumBrunnen (Franklin A. Graybill Statistical Laboratory) at Colorado State University; and M. Halford, J.L. Harris, J. Hoffman, B. Howell, D.W. Johnson, J. Lundquist, A. Schoettle and J.W. Schwandt of the USDA Forest Service. In addition, USDA Forest Service personnel from the Medicine Bow, Roosevelt and Shoshone NFs; W. Mack and D. Whyde (Bureau of Land Management); and E. Rhodenbaugh (Wind River Reservation, Bureau of Indian Affairs) provided assistance. Reviews of earlier versions of the manuscript were provided by E.L. Smith and M. Marsden. Helpful input by anonymous reviewers is appreciated. Funding and support for this project were provided by the USDA Forest Service Special Development and Technology Program (01-CS-11020000-088), USDA Forest Service Rocky Mountain Research Station (03-JV-11221605-298) and the Colorado Agricultural Experiment Station. NR 68 TC 3 Z9 3 U1 2 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1437-4781 EI 1439-0329 J9 FOREST PATHOL JI Forest Pathol. PD FEB PY 2014 VL 44 IS 1 BP 21 EP 38 DI 10.1111/efp.12065 PG 18 WC Forestry SC Forestry GA AA6KZ UT WOS:000331208500002 ER PT J AU Lynch, SC Zambino, PJ Scott, TA Eskalen, A AF Lynch, S. C. Zambino, P. J. Scott, T. A. Eskalen, A. TI Occurrence, incidence and associations among fungal pathogens and Agrilus auroguttatus, and their roles in Quercus agrifolia decline in California SO FOREST PATHOLOGY LA English DT Article ID CATALONIA NE SPAIN; COAST LIVE OAK; BOTRYOSPHAERIA-STEVENSII; SOUTHERN CALIFORNIA; CORK OAK; REGENERATION; CORTICOLA; CANKER; FOREST; SUBER AB Synchronous decline of oak (Quercus spp.) trees in woodlands has been described in Europe and eastern North America as a complex interaction of stressors that predispose, incite or contribute to tree death. This study presents a 2-year (2010-2011) assessment of the role of pathogens in coast live oak (Quercus agrifolia) woodlands in southern California where oak mortality occurs in locations that are infested and uninfested by the goldspotted oak borer (GSOB, Agrilus auroguttatus). Cumulative coast live oak mortality was not significantly different between sites and was weakly correlated with Diplodia corticola and GSOB incidence and negatively correlated with annual relative humidity. Multiple logistic regression models explained the presence of individual fungi or GSOB at the tree level. Fisher's exact test analysis determined that the presence of D.corticola, Fusarium solani, Dothiorella iberica, Cryptosporiopsis querciphila and Diatrypella verrucaeformis were each related to origin of sample location on tree, and C.querciphila was additionally related to symptom type on the bole. Multiple linear regression models showed high correlation between environmental variables and plot-level incidence of both GSOB and D.corticola. Disease incidence (DI) for D.corticola was highest in GSOB-uninfested locations. Jaccard index of association (J) showed that D.corticola was negatively associated with the presence of GSOB, F.solani and C.querciphila. Results suggest that oak decline in California is an example of a complex syndrome involving strong regional differences in factors that are associated with the problem. C1 [Lynch, S. C.] Univ Calif Riverside, Ctr Conservat Biol, Riverside, CA 92521 USA. [Lynch, S. C.; Eskalen, A.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Zambino, P. J.] US Forest Serv, USDA, Coeur Dalene, ID 83815 USA. [Scott, T. A.] Univ Calif Riverside, Dept Earth Sci, Riverside, CA 92521 USA. RP Eskalen, A (reprint author), Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. EM akif.eskalen@ucr.edu FU USDA Forest Service, Pacific Southwest Region FX We thank Tom Coleman and Andi Koonce (late) (U.S.D.A. Forest Service, Forest Health Protection) for coordination on sites for research. We also gratefully acknowledge the many organizations and their staff involved in granting permission to conduct research on public and private lands. These include the staff of the Cleveland National Forest Russell Lajoie, Owen Martin and Jeffrey Wells; staff of San Diego City and County Parks including Elise Lavallee, Roger Covalt, Mary Ramsey, James Stowers; Carole Bell from the Nature Conservancy; Jack Easton and Gail Egenes from the Riverside Land Conservancy; Dave Holt and Nick Sloan from Wilderness Gardens Preserve; David Hodges and Michael Hall from Mataguay Scout Camp, and Tom Powell. Wesley Tom, Melinda Ho, Lisa Yeung, Tina Cheung and Soner Akgul provided laboratory assistance and Karen Xu (UCR Statistical Consulting Collaboratory, University of California, Riverside) provided statistical analyses consulting and support. We gratefully acknowledge the anonymous reviewers for comments and suggestions. Special thanks to Beverlee Taub, Ashley Fiala, Joe Medeiros, Kris Preston, Michael Allen, Veronique Rorive. This project was funded (in part) by the USDA Forest Service, Pacific Southwest Region. NR 48 TC 6 Z9 6 U1 3 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1437-4781 EI 1439-0329 J9 FOREST PATHOL JI Forest Pathol. PD FEB PY 2014 VL 44 IS 1 BP 62 EP 74 DI 10.1111/efp.12070 PG 13 WC Forestry SC Forestry GA AA6KZ UT WOS:000331208500006 ER PT J AU Li, JB Sun, YD Liu, H Wang, YY Jia, YL Xu, MH AF Li, Jinbin Sun, Yiding Liu, Hui Wang, Yanyan Jia, Yulin Xu, Minghui TI Genetic variation and evolution of the Pit blast resistance locus in rice SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Blast disease; Evolution; Magnaporthe oryzae; Oryza sativa; Pit; Resistance gene ID DISEASE RESISTANCE; AVIRULENCE GENE; ORYZA-SATIVA; DNA POLYMORPHISM; TA; MARKERS; DIVERSITY; SELECTION; PROTEINS; SPECIFICITY AB Resistance (R) gene Pit in rice, encodes a protein with nucleotide binding sites-leucine rich repeat domain, prevents infections by strains of Magnaporthe oryzae in a gene for gene manner. Here, we analyzed the open reading frame (ORF) of Pit in 26 varieties including Oryza sativa L. subsp. indica Kato (Aus), indica (IND), temperate japonica (TEJ), tropical japonica (TRJ), aromatic (ARO) subgroups of Oryza sativa, and nine accessions of wild rice relatives including O. nivara and O. rufipogon from different growing areas. Twenty-one nucleotide differences were found within the ORFs of 35 accessions. Translation of these ORFs revealed a total of 11 Pit variants, seven of which were novel. Furthermore, variant H1 was identified in O. sativa and O. nivara; H2 and H10 were identified in O. sativa and O. rufipogon. H3, H4, and H11 were only found in O. sativa. H11 was the common type of TEJ and ARO; H3 and H4 was the specific type of TRJ and IND, respectively. H5, H6, and H7 were specific haplotypes of O. nivara, while, H8 and H9 were specific haplotypes of O. rufipogon. These results suggest that Pit is an ancient gene that exists before formation of rice domestication subgroups. C1 [Li, Jinbin] Yunnan Acad Agr Sci, Agr Environm & Resources Res Inst, Kunming 650223, Yunnan Province, Peoples R China. [Sun, Yiding; Wang, Yanyan; Xu, Minghui] Yunnan Acad Agr Sci, Inst Biotechnol & Genet Resources, Minist Agr, Key Lab Biotechnol Res Yunnan Prov,Key Lab Southw, Kunming 650223, Yunnan Province, Peoples R China. [Liu, Hui] Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Yunnan Province, Peoples R China. [Liu, Hui] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China. [Jia, Yulin] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. RP Xu, MH (reprint author), Yunnan Acad Agr Sci, Inst Biotechnol & Genet Resources, Minist Agr, Key Lab Biotechnol Res Yunnan Prov,Key Lab Southw, Kunming 650223, Yunnan Province, Peoples R China. EM Yulin.Jia@ars.usda.gov; xuminhui@sohu.com FU National Natural Science Foundation of China [31160355]; Natural Science Fund of Yunnan of China [2010ZC173] FX The authors gratefully thank Professor Wang Wen of Kunming Institute of Zoology of Chinese Academy of Sciences for providing genome sequences of rice germplasm; Harold Bockelman of the USDA-ARS National Small Grains Collection, Aberdeen, Idaho, USA for providing germplasm accessions; Zaiquan Cheng of Institute of Biotechnology and Genetic Resources, YAAS; Yiqing Guo of Yunnan Agricultural University for useful discussions; and Michael Lin, Tracy Bianco and Ellen McWhirter of USDA-ARS Dale Bumpers National Rice Research Center for pathogenicity assays and proof reading, respectively. This work was supported by the National Natural Science Foundation of China (31160355) and Natural Science Fund of Yunnan of China (2010ZC173). USDA is an equal opportunity provider and employer. NR 47 TC 3 Z9 3 U1 2 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 EI 1573-5109 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD FEB PY 2014 VL 61 IS 2 BP 473 EP 489 DI 10.1007/s10722-013-0051-8 PG 17 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AA2VX UT WOS:000330953400015 ER PT J AU Mezghani, N Zaouali, I Amri, WB Rouz, S Simon, PW Hannachi, C Ghrabi, Z Neffati, M Bouzbida, B Spooner, DM AF Mezghani, Najla Zaouali, Imen Amri, Wided Bel Rouz, Slim Simon, Philipp W. Hannachi, Cherif Ghrabi, Zeineb Neffati, Mohamed Bouzbida, Bechir Spooner, David M. TI Fruit morphological descriptors as a tool for discrimination of Daucus L. germplasm SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Daucus; Diversity; Multivariate analysis; Species; Tunisia ID CAROTA SUBSP SATIVUS; GENETIC-STRUCTURE; APIACEAE; CAPILLIFOLIUS; DIVERSITY AB Fruits present major morphological characters used to define genera and species within the Apiaceae. Northern Africa represents a major center of diversity of Daucus, with Tunisia containing at least 12 species and six subspecies. We assessed 14 mature fruit characters from the Daucus L. germplasm collection at the National Gene Bank of Tunisia. Quantification of variability for each character was investigated using the standardized Shannon-Weaver diversity index (H'). Diversity was established by factorial analysis of correspondence and cluster analysis. The computing H' index ranged from 0.31 for stylopodium shape to a maximum of 0.81 for spine shape. A mean diversity index for all traits recorded across all populations averaged 0.58 indicating existence of an important genetic diversity within the collection. Multivariate analysis of factorial correspondence and cluster analysis on morphological descriptors permitted the subdivision of the Daucus collection into five distinct groups including one single accession group, two groups with six accessions, one group of nine accessions and one large group with 81 accessions corresponding each one to a species among Daucus except the large group corresponding to D. carota and D. capillifolius. The grouping of populations did not reflect bioclimatic and geographic patterns, suggesting adaptation of populations to local environments. Of equal importance, our study shows the effectiveness of fruit characters alone to identify species in this collection of Daucus. C1 [Mezghani, Najla; Zaouali, Imen; Amri, Wided Bel] Banque Natl Genes, Tunis 1080, Tunisia. [Amri, Wided Bel] Fac Sci Tunis, Tunis 1060, Tunisia. [Rouz, Slim] Inst Super Agron Mogran, Mogran, Tunisia. [Simon, Philipp W.; Spooner, David M.] Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. [Hannachi, Cherif] Inst Super Agron Chott Mariem, Chott Mariem, Tunisia. [Ghrabi, Zeineb] Inst Natl Agron Tunisie, Tunis, Tunisia. [Neffati, Mohamed; Bouzbida, Bechir] Inst Reg Arides, Medenine 4119, Tunisia. RP Mezghani, N (reprint author), Banque Natl Genes, Blvd Leader Yasser Arafat ZI Charguia 1, Tunis 1080, Tunisia. EM najla_mezghani@yahoo.fr; david.spooner@ars.usda.gov NR 32 TC 4 Z9 4 U1 0 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 EI 1573-5109 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD FEB PY 2014 VL 61 IS 2 BP 499 EP 510 DI 10.1007/s10722-013-0053-6 PG 12 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AA2VX UT WOS:000330953400017 ER PT J AU Li, Y Cheng, RY Spokas, KA Palmer, AA Borevitz, JO AF Li, Yan Cheng, Riyan Spokas, Kurt A. Palmer, Abraham A. Borevitz, Justin O. TI Genetic Variation for Life History Sensitivity to Seasonal Warming in Arabidopsis thaliana SO GENETICS LA English DT Article DE Arabidopsis; genome-wide association studies (GWAS); flowering time; growing season; climate change ID ANTHROPOGENIC CLIMATE-CHANGE; QUANTITATIVE TRAIT LOCI; FLOWERING TIME; RANGE SHIFTS; PHENOTYPIC PLASTICITY; NATURAL VARIATION; AIR-TEMPERATURE; ADAPTATION; RESPONSES; EVOLUTIONARY AB Climate change has altered life history events in many plant species; however, little is known about genetic variation underlying seasonal thermal response. In this study, we simulated current and three future warming climates and measured flowering time across a globally diverse set of Arabidopsis thaliana accessions. We found that increased diurnal and seasonal temperature (1 degrees-3 degrees) decreased flowering time in two fall cohorts. The early fall cohort was unique in that both rapid cycling and overwintering life history strategies were revealed; the proportion of rapid cycling plants increased by 3-7% for each 1 degrees temperature increase. We performed genome-wide association studies (GWAS) to identify the underlying genetic basis of thermal sensitivity. GWAS identified five main-effect quantitative trait loci (QTL) controlling flowering time and another five QTL with thermal sensitivity. Candidate genes include known flowering loci; a cochaperone that interacts with heat-shock protein 90; and a flowering hormone, gibberellic acid, a biosynthetic enzyme. The identified genetic architecture allowed accurate prediction of flowering phenotypes (R-2 > 0.95) that has application for genomic selection of adaptive genotypes for future environments. This work may serve as a reference for breeding and conservation genetic studies under changing environments. C1 [Li, Yan; Borevitz, Justin O.] Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA. [Cheng, Riyan; Palmer, Abraham A.] Univ Chicago, Dept Human Genet, Chicago, IL 60637 USA. [Palmer, Abraham A.] Univ Chicago, Dept Psychiat & Behav Neurosci, Chicago, IL 60637 USA. [Li, Yan] Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China. [Li, Yan] Minist Agr, Key Lab Soybean Biol & Genet Improvement, Nanjing, Jiangsu, Peoples R China. [Cheng, Riyan; Borevitz, Justin O.] Australian Natl Univ, Res Sch Biol, Canberra, ACT 0200, Australia. [Spokas, Kurt A.] USDA ARS, St Paul, MN 55108 USA. RP Borevitz, JO (reprint author), Australian Natl Univ, Res Sch Biol, Canberra, ACT 0200, Australia. EM justin.borevitz@anu.edu.au RI Borevitz, Justin/B-5423-2012; Palmer, Abraham/L-2158-2014; Spokas, Kurt/F-4839-2016; OI Palmer, Abraham/0000-0003-3634-0747; Spokas, Kurt/0000-0002-5049-5959; Li, Yan/0000-0002-1627-7763 FU National Institutes of Health (NIH) [R01GM073822]; NIH [R01DA021336, R01MH079103, R21DA024845]; Australian National University FX We thank John Zdenek and Sandra A. M. Suwanski at the University of Chicago greenhouse for help in preparing soils and watering plants, Eleni Boikoglou for collaborating on preparing the simulated weather files, and Nina Noah and Yi Ren for their help in scanning barcodes. This work was funded by National Institutes of Health (NIH) grant R01GM073822 (to J.O.B. and Y.L.) and NIH grants R01DA021336, R01MH079103, and R21DA024845 (to A.A.P. and R.C.) and Australian National University startup funds (to J.O.B. and R.C.). NR 40 TC 23 Z9 23 U1 4 U2 53 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD FEB PY 2014 VL 196 IS 2 BP 569 EP + DI 10.1534/genetics.113.157628 PG 18 WC Genetics & Heredity SC Genetics & Heredity GA AA7KX UT WOS:000331277400018 PM 24281156 ER PT J AU Yu, Q Li, LH Luo, QY Eamus, D Xu, SH Chen, C Wang, EL Liu, JD Nielsen, DC AF Yu, Qiang Li, Longhui Luo, Qunying Eamus, Derek Xu, Shouhua Chen, Chao Wang, Enli Liu, Jiandong Nielsen, David C. TI Year patterns of climate impact on wheat yields SO INTERNATIONAL JOURNAL OF CLIMATOLOGY LA English DT Article ID CENTRAL GREAT-PLAINS; CROP YIELDS; SOUTHERN-OSCILLATION; UNITED-STATES; RICE YIELD; VARIABILITY; MODEL; CALIFORNIA; APSIM; AUSTRALIA C1 [Yu, Qiang; Li, Longhui; Luo, Qunying; Eamus, Derek; Chen, Chao] Univ Technol Sydney, Plant Funct Biol & Climate Change Cluster, Broadway, NSW 2007, Australia. [Xu, Shouhua] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. [Chen, Chao] Columbia Univ, Earth Inst, Int Res Inst Climate & Soc, Palisades, NY 10964 USA. [Wang, Enli] CSIRO Land & Water APSRU, Canberra, ACT, Australia. [Liu, Jiandong] Chinese Acad Meteorol Sci, Ctr Agometeorol, Beijing, Peoples R China. [Nielsen, David C.] ARS, Cent Great Plains Res Stn, USDA, Arkon, CO USA. RP Yu, Q (reprint author), Univ Technol Sydney, Plant Funct Biol & Climate Change Cluster, POB 123, Broadway, NSW 2007, Australia. EM Qiang.Yu@uts.edu.au RI Nielsen, David/A-8044-2009; Chen, Chao/A-8242-2015; Wang, Enli/K-7478-2012; Yu, Qiang/D-3702-2009; OI Nielsen, David/0000-0002-8240-7183; Wang, Enli/0000-0002-6653-5791; Yu, Qiang/0000-0001-6950-1821; Eamus, Derek/0000-0003-2765-8040 FU National Basic Research Program of China [2012CB955304] FX This research was supported by National Basic Research Program of China (No. 2012CB955304). NR 49 TC 4 Z9 6 U1 2 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0899-8418 EI 1097-0088 J9 INT J CLIMATOL JI Int. J. Climatol. PD FEB PY 2014 VL 34 IS 2 BP 518 EP 528 PG 11 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA AA6CU UT WOS:000331187200019 ER PT J AU Ayars, JE Soppe, RWO AF Ayars, James E. Soppe, Richard W. O. TI INTEGRATED ON-FARM DRAINAGE MANAGEMENT FOR DRAINAGE WATER DISPOSAL SO IRRIGATION AND DRAINAGE LA English DT Article DE serial biological concentration; drainage water disposal; drainage; drainage water reuse; integrated drainage management ID EVAPORATION PONDS; SALINE WATER; COTTON; IRRIGATION; QUALITY; TABLE AB Providing environmentally safe methods for disposal of drainage water containing salt and nutrients is a challenge for irrigated agriculture. A system developed for sequentially using saline drainage water for supplemental irrigation resulted in significant reduction of the drainage water volume. This system dubbed integrated on-farm drainage management' (IFDM) was demonstrated on four 65-ha fields located on a farm on the west side of the San Joaquin Valley of California. Three of the fields were used to grow salt-sensitive crops (tomato, garlic) and the fourth was used to grow salt-tolerant crops, e.g. Jose' tall wheatgrass. Subsurface drainage systems were installed on all fields at a maximum depth of 1.8m and had controls to regulate shallow water table position and drainage flow. The total drainage flow from the site represented 0.7% of the applied water. The area used for salt-tolerant crops was less than 6% of the total area served, compared to using evaporation ponds requiring areas equal to or greater than 10% of the served area. The results demonstrated that the regional groundwater quality masked the concentrating effect of crop water use. Deep percolation from the fields contributing to the reuse area ranged from 6 to 10% of the total applied water. Published 2013. This article is a U.S. Government work and is in the public domain in the USA. C1 [Ayars, James E.] USDA ARS, Parlier, CA 93648 USA. [Soppe, Richard W. O.] Int Ctr Agr Res Dry Areas, Amman, Jordan. RP Ayars, JE (reprint author), USDA ARS, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM james.ayars@ars.usda.gov RI Soppe, Richard/A-9581-2010 OI Soppe, Richard/0000-0001-6751-8507 FU California Department of Water Resources FX The authors thank the California Department of Water Resources for funding the project and Mr John Diener who permitted the installation of this project on his property and has supported the operation. NR 24 TC 0 Z9 0 U1 0 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1531-0353 EI 1531-0361 J9 IRRIG DRAIN JI Irrig. Drain. PD FEB PY 2014 VL 63 IS 1 BP 102 EP 111 DI 10.1002/ird.1771 PG 10 WC Agronomy; Water Resources SC Agriculture; Water Resources GA AA5HY UT WOS:000331127900011 ER PT J AU Sanchez, NCB Carroll, JA Randel, RD Vann, RC Welsh, TH AF Sanchez, N. C. Burdick Carroll, J. A. Randel, R. D. Vann, R. C. Welsh, T. H., Jr. TI Associations between endotoxin-induced metabolic changes and temperament in Brahman bulls SO JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION LA English DT Article ID RECTAL TEMPERATURE; CARBOHYDRATE-METABOLISM; GLUCOSE-PRODUCTION; TECHNICAL NOTE; CATTLE; STRESS; LIPOPOLYSACCHARIDE; CORTISOL; BEHAVIOR; HOMEOSTASIS C1 [Sanchez, N. C. Burdick; Carroll, J. A.] USDA ARS, Livestock Issues Res Unit, Lubbock, TX 79403 USA. [Randel, R. D.] Texas A&M Syst Overton, Texas AgriLife Res, Overton, TX USA. [Vann, R. C.] Mississippi State Univ Raymond, Brown Loam Branch Expt Stn, Canton, MS USA. [Welsh, T. H., Jr.] Texas A&M Syst Coll Stn, Texas AgriLife Res, Dept Anim Sci, College Stn, TX USA. RP Carroll, JA (reprint author), USDA ARS, Livestock Issues Res Unit, Lubbock, TX 79403 USA. EM jeff.carroll@ars.usda.gov FU Texas AgriLife Research, USDA-NRI-CSREES Grant [2005-01671]; USDA SG [2006-34564- 1700] FX The authors acknowledge the technical support of D.A. Neuendorff, A.W. Lewis, L.C. Caldwell, A.N. Loyd, J. Lyons, J. W. Dailey, C. Wu and J. C. Carroll. This study was supported in part by Texas AgriLife Research, USDA-NRI-CSREES Grant (grant number 2005-01671) and USDA SG (grant number 2006-34564- 1700) NR 40 TC 5 Z9 5 U1 1 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2439 EI 1439-0396 J9 J ANIM PHYSIOL AN N JI J. Anim. Physiol. Anim. Nutr. PD FEB PY 2014 VL 98 IS 1 BP 178 EP 190 PG 13 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA AA0PZ UT WOS:000330799600020 ER PT J AU Fratamico, PM Wasilenko, JL Garman, B DeMarco, DR Varkey, S Jensen, M Rhoden, K Tice, G AF Fratamico, Pina M. Wasilenko, Jamie L. Garman, Bradley DeMarco, Daniel R. Varkey, Stephen Jensen, Mark Rhoden, Kyle Tice, George TI Evaluation of a Multiplex Real-Time PCR Method for Detecting Shiga Toxin-Producing Escherichia coli in Beef and Comparison to the US Department of Agriculture Food Safety and Inspection Service Microbiology Laboratory Guidebook Method SO JOURNAL OF FOOD PROTECTION LA English DT Article ID SEROGROUPS O26; UNITED-STATES; GROUND-BEEF; CATTLE; GENES; O121; O111; O103; O145; EAE AB The "top-six" non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, and O145) most frequently associated with outbreaks and cases of foodborne illnesses have been declared as adulterants in beef by the U.S. Department of Agriculture Food Safety and Inspection Service (FSIS). Regulatory testing in beef began in June 2012. The purpose of this study was to evaluate the DuPont BAX System method for detecting these top six STEC strains and strains of E. coli O157:H7. For STEC, the BAX System real-time STEC suite was evaluated, including a screening assay for the stx and eae virulence genes and two panel assays to identify the target serogroups: panel 1 detects O26, O111, and O121, and panel 2 detects O45, O103, O145. For E. coli O157:H7, the BAX System real-time PCR assay for this specific serotype was used. Sensitivity of each assay for the PCR targets was >= 1.23 x 10(3) CFU/ml in pure culture. Each assay was 100% inclusive for the strains tested (20 to 50 per assay), and no cross-reactivity with closely related strains was observed in any of the assays. The performance of the BAX System methods was compared with that of the FSIS Microbiology Laboratory Guidebook (MLG) methods for detection of the top six STEC and E. coli O157:H7 strains in ground beef and beef trim. Generally, results of the BAX System method were similar to those of the MLG methods for detecting non-O157 STEC and E. coli O157:H7. Reducing or eliminating novobiocin in modified tryptic soy broth (mTSB) may improve the detection of STEC O111 strains; one beef trim sample inoculated with STEC O111 produced a negative result when enriched in mTSB with 8 mg/liter novobiocin but was positive when enriched in mTSB without novobiocin. The results of this study indicate the feasibility of deploying a panel of real-time PCR assay configurations for the detection and monitoring of the top six STEC and E. coli O157:H7 strains in beef. The approach could easily be adapted for additional multiplex assays should regulations expand to include other O serogroups or virulence genes. C1 [Fratamico, Pina M.; Wasilenko, Jamie L.; Garman, Bradley] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Wasilenko, Jamie L.] ARS, USDA, Richard Russell Res Ctr, Athens, GA 30605 USA. [DeMarco, Daniel R.; Varkey, Stephen; Jensen, Mark; Rhoden, Kyle; Tice, George] DuPont Nutr & Hlth, DuPont Expt Stn, Wilmington, DE 19880 USA. RP Fratamico, PM (reprint author), ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM pina.fratamico@ars.usda.gov NR 22 TC 12 Z9 12 U1 3 U2 30 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2014 VL 77 IS 2 BP 180 EP 188 DI 10.4315/0362-028X.JFP-13-248 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AA0WY UT WOS:000330819300001 PM 24490911 ER PT J AU Donado-Godoy, P Clavijo, V Leon, M Arevalo, A Castellanos, R Bernal, J Tafur, M Ovalle, MV Alali, WQ Hume, M Romero-Zuniga, JJ Walls, I Doyle, MP AF Donado-Godoy, Pilar Clavijo, Viviana Leon, Maribel Arevalo, Alejandra Castellanos, Ricardo Bernal, Johan Tafur, Mc Allister Victoria Ovalle, Maria Alali, Walid Q. Hume, Michael Jose Romero-Zuniga, Juan Walls, Isabel Doyle, Michael P. TI Counts, Serovars, and Antimicrobial Resistance Phenotypes of Salmonella on Raw Chicken Meat at Retail in Colombia SO JOURNAL OF FOOD PROTECTION LA English DT Article ID CAMPYLOBACTER SPP.; ORGANIC CHICKENS; RISK-ASSESSMENT; PREVALENCE; POULTRY; SUSCEPTIBILITY; CARCASSES; PRODUCTS; HUMANS; STATES AB The objective of this study was to determine Salmonella counts, serovars, and antimicrobial-resistant phenotypes on retail raw chicken carcasses in Colombia. A total of 301 chicken carcasses were collected from six departments (one city per department) in Colombia. Samples were analyzed for Salmonella counts using the most-probable-number method as recommended by the U.S. Department of Agriculture, Food Safety Inspection Service protocol. A total of 378 isolates (268 from our previous study) were serotyped and tested for antimicrobial susceptibility. The overall Salmonella count (mean log most probable number per carcass +/- 95% confidence interval) and prevalence were 2.1(2.0 to 2.3) and 37%, respectively. There were significant differences (P < 0.05) by Salmonella levels (i.e., counts and prevalence) by storage temperature (i.e., frozen, chilled, or ambient), retail store type (wet markets, supermarkets, and independent markets), and poultry company (chicken produced by integrated or nonintegrated company). Frozen chicken had the lowest Salmonella levels compared with chicken stored at other temperatures, chickens from wet markets had higher levels than those from other retail store types, and chicken produced by integrated companies had lower levels than nonintegrated companies. Thirty-one Salmonella serovars were identified among 378 isolates, with Salmonella Paratyphi B tartrate-positive (i.e., Salmonella Paratyphi B dT +) the most prevalent (44.7%), followed by Heidelberg (19%), Enteritidis (17.7%), Typhimurium (5.3%), and Anatum (2.1%). Of all the Salmonella isolates, 35.2% were resistant to 1 to 5 antimicrobial agents, 24.6% to 6 to 10, and 33.9% to 11 to 15. Among all the serovars obtained, Salmonella Paratyphi B dT + and Salmonella Heidelberg were the most antimicrobial resistant. Salmonella prevalence was determined to be high, whereas cell numbers were relatively low. These data can be used in developing risk assessment models for preventing the transmission of Salmonella from chicken to humans in Colombia. C1 [Donado-Godoy, Pilar; Clavijo, Viviana; Arevalo, Alejandra; Castellanos, Ricardo; Bernal, Johan; Victoria Ovalle, Maria] CBB, CORPOICA Corp Colombiana Invest Agr, Cundinamarca, Colombia. [Leon, Maribel; Tafur, Mc Allister] ICA, Bogota, Colombia. [Alali, Walid Q.; Doyle, Michael P.] Univ Georgia, Ctr Food Safety, Griffin, GA 30223 USA. [Hume, Michael] ARS, USDA, Food Anim Protect Res Lab, College Stn, TX 77845 USA. [Jose Romero-Zuniga, Juan] Univ Nacl Costa Rica, Programa Invest Med Poblac, Escuela Med Vet, Heredia, Costa Rica. [Walls, Isabel] Natl Inst Food & Agr, USDA, Washington, DC 20250 USA. RP Alali, WQ (reprint author), Univ Georgia, Ctr Food Safety, 1109 Expt St, Griffin, GA 30223 USA. EM walali@uga.edu OI Walls, Isabel/0000-0002-9643-8845 FU University of Georgia; World Health Organization Global Foodborne Infections Network FX This research work was supported by the project "Data Collection for Salmonella in Raw Poultry in Colombia" of the University of Georgia, in collaboration with the World Health Organization Global Foodborne Infections Network. We thank Dr. Enrique Perez from Pan American Health Organization-World Health Organization for facilitating the collaboration between the research groups that worked on this project. We thank Dr. Maria Ines and Dr. Marcelo Galas of Malbran Institute for their advice regarding antimicrobial resistance and serotyping of Salmonella. NR 35 TC 4 Z9 4 U1 0 U2 8 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2014 VL 77 IS 2 BP 227 EP 235 DI 10.4315/0362-028X.JFP-13-276 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AA0WY UT WOS:000330819300006 PM 24490916 ER PT J AU Jones, SA Salter, RS Goldsmith, T Quintana, J Rapnicki, P Shuck, K Wells, JE Schneider, MJ Griffin, D AF Jones, Shuna A. Salter, Robert S. Goldsmith, Tim Quintana, Julio Rapnicki, Paul Shuck, Karen Wells, Jim E. Schneider, Marilyn J. Griffin, Dee TI Development and Model Testing of Antemortem Screening Methodology To Predict Required Drug Withholds in Heifers SO JOURNAL OF FOOD PROTECTION LA English DT Article ID BEEF KIDNEY JUICE; RESIDUES; SERUM; FLUNIXIN; ANTIBIOTICS; ANIMALS; URINE AB A simple, cow-side test for the presence of drug residues in live animal fluids would provide useful information for tissue drug residue avoidance programs. This work describes adaptation and evaluation of rapid screening tests to detect drug residues in serum and urine. Medicated heifers had urine, serum, and tissue biopsy samples taken while on drug treatment. Samples were tested by rapid methods and high-performance liquid chromatography (HPLC). The adapted microbial inhibition method, kidney inhibition swab test, was useful in detecting sulfadimethoxine in serum, and its response correlated with the prescribed withdrawal time for the drug, 5 to 6 days posttreatment. The lateral flow screening method for flunixin and beta-lactams, adapted for urine, was useful in predicting flunixin in liver detected by HPLC, 96 h posttreatment. The same adapted methods were not useful to detect ceftiofur in serum or urine due to a lack of sensitivity at the levels of interest. These antemortem screening test studies demonstrated that the method selected, and the sampling matrix chosen (urine or serum), will depend on the drug used and should be based on animal treatment history if available. The live animal tests demonstrated the potential for verification that an individual animal is free of drug residues before sale for human consumption. C1 [Jones, Shuna A.; Wells, Jim E.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Jones, Shuna A.; Shuck, Karen; Griffin, Dee] Univ Nebraska Lincoln, Great Plains Vet Educ Ctr, Clay Ctr, NE 68933 USA. [Salter, Robert S.; Quintana, Julio] Charm Sci Inc, Lawrence, MA 01843 USA. [Goldsmith, Tim] Univ Minnesota, Coll Vet Med, Ctr Anim Hlth & Food Safety, Minneapolis, MN 55455 USA. [Rapnicki, Paul] Elanco Anim Hlth, Greenfield, IN 46140 USA. [Schneider, Marilyn J.] USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Jones, SA (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM shuna.jones@ars.usda.gov NR 18 TC 2 Z9 2 U1 1 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2014 VL 77 IS 2 BP 292 EP 298 DI 10.4315/0362-028X.JFP-13-267 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AA0WY UT WOS:000330819300014 PM 24490924 ER PT J AU Harrison, RL Keena, MA Rowley, DL AF Harrison, Robert L. Keena, Melody A. Rowley, Daniel L. TI Classification, genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from Asia, Europe, and North America SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Baculovirus; Nucleopolyhedrovirus; Gypsy moth; Lymantria dispar; Genome; LdMNPV ID NUCLEAR-POLYHEDROSIS-VIRUS; BOMBYX-MORI-NUCLEOPOLYHEDROVIRUS; AUTOGRAPHA-CALIFORNICA NUCLEOPOLYHEDROVIRUS; GENOMIC SEQUENCE-ANALYSIS; REPUBLIC-OF-CHINA; GYPSY-MOTH; MULTIPLE-NUCLEOPOLYHEDROVIRUS; MOLECULAR-IDENTIFICATION; PHYLOGENETIC ANALYSIS; LEPIDOPTERA AB Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) has been formulated and applied to control outbreaks of the gypsy moth, L. dispar. To classify and determine the degree of genetic variation among isolates of L. dispar NPVs from different parts of the range of the gypsy moth, partial sequences of the lef-8, lef-9, and polh genes were determined for Lymantria spp. virus samples from host populations throughout the world. Sequence analysis confirmed that all L. dispar virus samples tested contained isolates of the species Lymantria dis par multiple nucleopolyhedrovirus (Baculoviridae: Alphabaculovirus). Phylogenetic inference based on the lef-8 sequences indicated that the LdMNPV isolates formed two groups, one consisting primarily of isolates from Asia, and one consisting primarily of isolates from Europe and North America. The complete genome sequence was determined for an isolate from the Asian group, LdMNPV-2161 (S. Korea). The LdMNPV-2161 genome was 163,138 bp in length, 2092 bp larger than the previously determined genome of LdMNPV isolate 5-6 (CT, USA). The two genome sequences were co-linear, with an overall nucleotide sequence identity of 97.5% and some differences in ORF content. In droplet-feeding bioassays against neonate L dispar larvae, isolates LdMNPV-3029 (Virin-ENSh/Russia) and LdMNPV-Ab-a624 (MA, USA) killed neonate larvae with an LC50 values that were 1.8- to 3.2-fold lower than a sample of Gypchek (R) (CT, USA) and isolates LdMNPV-3041 (Japan) and LdMNPV-2161. This study expands our knowledge about genetic variation among LdMNPV isolates and provides novel information on the distinct groups in which these NPVs occur. Published by Elsevier Inc. C1 [Harrison, Robert L.; Rowley, Daniel L.] ARS, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Keena, Melody A.] US Forest Serv, No Res Stn, USDA, Hamden, CT 06514 USA. RP Harrison, RL (reprint author), ARS, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr, USDA,BARC W, Bldg 011A,Room 214,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Robert.L.Harrison@ars.usda.gov; mkeena@fs.fed.us; Daniel.Rowley@ars.usda.gov RI Harrison, Robert/I-4707-2014; OI Harrison, Robert/0000-0002-8348-3874 FU U.S. Department of Agriculture FX We wish to thank the anonymous reviewers of this manuscript. This work was supported by the U.S. Department of Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 63 TC 7 Z9 7 U1 2 U2 26 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD FEB PY 2014 VL 116 BP 27 EP 35 DI 10.1016/j.jip.2013.12.005 PG 9 WC Zoology SC Zoology GA AA8NI UT WOS:000331351800005 PM 24370838 ER PT J AU Liao, KH Zhu, Q Doolittle, J AF Liao Kai-hua Zhu Qing Doolittle, James TI Temporal stability of apparent soil electrical conductivity measured by electromagnetic induction techniques SO JOURNAL OF MOUNTAIN SCIENCE LA English DT Article DE Geophysics; Pedology; Soil hydrology; Soil water content ID WATER-CONTENT; AGRICULTURAL LANDSCAPE; PRECISION AGRICULTURE; MOISTURE; PATTERNS; SCALE; BOUNDARIES; CATCHMENT; MANGANESE; ACCURACY AB Assessing and managing the spatial variability of hydropedological properties are important in environmental, agricultural, and geological sciences. The spatial variability of soil apparent electrical conductivity (ECa) measured by electromagnetic induction (EMI) techniques has been widely used to infer the spatial variability of hydrological and pedological properties. In this study, temporal stability analysis was conducted for measuring repeatedly soil ECa in an agricultural landscape in 2008. Such temporal stability was statistically compared with the soil moisture, terrain indices (slope, topographic wetness index (TWI), and profile curvature), and soil properties (particle size distribution, depth to bedrock, Mn mottle content, and soil type). Locations with great and temporally unstable soil ECa were also associated with great and unstable soil moisture, respectively. Soil ECa were greater and more unstable in the areas with great TWI (TWI > 8), gentle and concave slope (slope < 3%; profile curvature > 0.2). Soil ECa exponentially increased with depth to bedrock, and soil profile silt and Mn mottle contents (R-2 = 0.57), quadratically (R-2 = 0.47), and linearly (R-2 = 0.47), respectively. Soil ECa was greater and more unstable in Gleysol and Nitosol soils, which were distributed in areas with low elevation (< 380 m), thick soil solum (> 3 m), and fluctuated water table (shallow in winter and spring but deep in summer and fall). In contrast, Acrisol, Luvisol, and Cambisol soils, which are distributed in the upper slope areas, had lower and more stable soil ECa. Through these observations, we concluded that the temporal stability of soil ECa can be used to interpret the spatial and temporal variability of these hydropedological properties. C1 [Liao Kai-hua; Zhu Qing] Chinese Acad Sci, Nanjing Inst Geog & Limnol, State Key Lab Lake Sci & Environm, Nanjing 210008, Peoples R China. [Doolittle, James] Natl Soil Survey Ctr, USDA NRCS, Newtown Sq, PA 19073 USA. RP Zhu, Q (reprint author), Chinese Acad Sci, Nanjing Inst Geog & Limnol, State Key Lab Lake Sci & Environm, 73 East Beijing Rd, Nanjing 210008, Peoples R China. EM liaokaihua1984@163.com; qzhu@niglas.ac.cn FU USDA National Research Initiative [2002-35102-12547]; National Natural Science Foundation of China [41271109]; Leading Edge Project of Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences [NIGLAS2011YJ01] FX The data collection of this study was supported by the USDA National Research Initiative (Grant No. 2002-35102-12547). The preparation of this paper was supported by National Natural Science Foundation of China (Grant No. 41271109) and Leading Edge Project of Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences (Grant No. NIGLAS2011YJ01). NR 48 TC 1 Z9 1 U1 2 U2 19 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1672-6316 EI 1993-0321 J9 J MT SCI-ENGL JI J Mt. Sci. PD FEB PY 2014 VL 11 IS 1 BP 98 EP 109 DI 10.1007/s11629-012-2630-0 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA AA0KD UT WOS:000330783100009 ER PT J AU Sen, S Iyer, C Meydani, SN AF Sen, S. Iyer, C. Meydani, S. N. TI Obesity during pregnancy alters maternal oxidant balance and micronutrient status SO JOURNAL OF PERINATOLOGY LA English DT Article DE inflammation; pregnancy; micronutrients; obesity; prenatal vitamins ID BODY-MASS INDEX; INCREASED OXIDATIVE STRESS; BIRTH-DEFECTS; ANTIOXIDANT SUPPLEMENTATION; LIQUID-CHROMATOGRAPHY; INFLAMMATORY MARKERS; SHORT-TERM; PLASMA; RISK; ADIPOSITY AB OBJECTIVE: Little is known about the effect of obesity on inflammatory status in pregnant women. The objective of this study was to determine the effect of obesity on markers of inflammation, oxidative stress and micronutrient status in obese pregnant women and their infants compared with lean controls (Lc). STUDY DESIGN: This was a prospective case-control study. A total of 15 obese (Ob; body mass index (BMI) >30 kg m(-2)) and 15 lean (BMI 18-25 kg m(-2)) women were recruited based on prepregnancy BMI. Vitamins A, B-6, C, E and 25-hydroxyvitamin D (25(OH)D), zinc, red blood cell (RBC) folate, C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha and oxidized and reduced glutathione were measured from maternal blood between 24 and 28 weeks of gestation. Vitamins A, B-6, C and E, 25(OH)D, zinc, red blood cell folate, CRP and IL-6 were measured from cord blood at delivery. RESULT: Ob pregnant women have statistically significantly lower levels of vitamin B-6, vitamin C, vitamin E, RBC folate, higher CRP and IL-6 levels and higher ratio of oxidized to reduced glutathione compared with Lc pregnant women. Infants born to Ob mothers did not have statistically significantly higher measures of inflammation or oxidative stress. There were no differences in micronutrient concentrations between Lc and Ob infants, but folate, vitamin B-6 and zinc levels correlated strongly between mother and infant. There was no statistically significant difference in any parameter between Ob and Lc cord blood. CONCLUSION: Ob pregnant women have increased inflammation and oxidative stress, and lower levels of nutritional antioxidant defenses compared with Lc pregnant women. We speculate that lower antioxidant defenses combined with increased oxidative stress and inflammation may contribute to the adverse outcomes associated with pregnancy in Ob women. C1 [Sen, S.; Meydani, S. N.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Sen, S.] Tufts Med Ctr, Mother Infant Res Inst, Boston, MA 02111 USA. [Sen, S.] Tufts Med Ctr, Dept Pediat, Div Newborn Med, Boston, MA 02111 USA. [Iyer, C.] Tufts Med Ctr, Dept Obstet & Gynecol, Boston, MA 02111 USA. RP Sen, S (reprint author), Tufts Med Ctr, Dept Pediat, Div Newborn Med, 800 Washington St,TMC Box 44, Boston, MA 02111 USA. EM ssen@tuftsmedicalcenter.org FU Tufts Medical Center Research Grant; Natalie Zucker Research Grant; USDA [58-1950-7-707] FX This research was funded by the Tufts Medical Center Research Grant, The Natalie Zucker Research Grant and USDA contract no. 58-1950-7-707. NR 53 TC 12 Z9 12 U1 0 U2 8 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0743-8346 EI 1476-5543 J9 J PERINATOL JI J. Perinatol. PD FEB PY 2014 VL 34 IS 2 BP 105 EP 111 DI 10.1038/jp.2013.153 PG 7 WC Obstetrics & Gynecology; Pediatrics SC Obstetrics & Gynecology; Pediatrics GA AA5KU UT WOS:000331138400005 PM 24355940 ER PT J AU Novak, JM Cantrell, KB Watts, DW Busscher, WJ Johnson, MG AF Novak, Jeffrey M. Cantrell, Keri B. Watts, Donald W. Busscher, Warren J. Johnson, Mark G. TI Designing relevant biochars as soil amendments using lignocellulosic-based and manure-based feedstocks SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Article DE Carbon sequestration; Designer biochar; Feedstocks; Leaching; Nutrients; Pyrolysis ID COASTAL-PLAIN SOIL; CARBON SEQUESTRATION; CHEMICAL-PROPERTIES; WATER-QUALITY; PLANT-GROWTH; CHARCOAL; PYROLYSIS; TEMPERATURE; FERTILITY; FERRALSOL AB Biochars are a by-product of the biofuel processing of lignocellulosic and manure feedstocks. Because biochars contain an assemblage of organic and inorganic compounds, they can be used as an amendment for C sequestration and soil quality improvement. However, not all biochars are viable soil amendments; this is because their physical and chemical properties vary due to feedstock elemental composition, biofuel processing, and particle size differences. Biochar could deliver a more effective service as a soil amendment if its chemistry was designed ex ante with characteristics that target specific soil quality issues. In this study, we demonstrate how biochars can be designed with relevant properties as successful soil amendments through feedstock selection, pyrolysis conditions, and particle size choices. Biochars were produced by pyrolysis of parent lignocellulosic feedstock sources-peanut hull (PH; Archis hypogaea), pecan shell (PS; Carya illinoensis), switchgrass (SG; Panicum virgatum), pine chips (PC; Pinus taeda), hardwood wastes (wood), and poultry litter manure (PL; Gallus domesticus), as well as blends of these feedstocks at temperatures ranging from 250 to 700 A degrees C. Additionally, blended feedstocks were made into pellets (> 2 mm) prior to pyrolysis at 350 A degrees C. Dust-sized (< 0.42 mm) biochar was obtained through grinding of pelletized biochars. After chemical characterization, the biochars were evaluated as fertility amendments in a Norfolk soil (fine-loamy, kaolinitic, thermic, Typic Kandiudult) during two different pot incubation experiments. PL biochars were alkaline and enriched in N and P, whereas biochar from lignocellulosic feedstocks exhibited mixed pH and nutrient contents. Blending PL with PC resulted in lower biochar pH values and nutrient contents. In pot experiment 1, most biochars significantly (P < 0.05) raised soil pH, soil organic carbon, cation exchange capacity, and Mehlich 1 extractable P and K. PL biochar added at 20 g kg(-1) resulted in excessive soil P concentrations (393 to 714 mg kg(-1)) and leachate enriched with dissolved phosphorus (DP, 22 to 70 mg L-1). In pot experiment 2, blended and pelletized PL with PC feedstock reduced soil pH and extractable soil P and K concentrations compared to pot experiment 1. Water leachate DP concentrations were significantly (P < 0.05) reduced by pelletized biochar blends. Short-term laboratory pot experiments revealed that biochars can have different impacts at modifying soil quality characteristics. Keying on these results allowed for creating designer biochars to address specific soil quality limitations. In the process of manufacturing designer biochars, first, it is important to know what soil quality characteristics are in need of change. Second, choices between feedstocks, blends of these feedstocks, and their accompanying particle sizes can be made prior to pyrolysis to create biochars tailored for addressing specific soil quality improvements. Utilization of these principles should allow for effective service of the designed biochar as a soil amendment while minimizing unwanted ex facto soil quality changes and environmental effects. C1 [Novak, Jeffrey M.; Cantrell, Keri B.; Watts, Donald W.; Busscher, Warren J.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Johnson, Mark G.] US EPA, Western Ecol Div, Natl Hlth & Environm Effects Res Lab, Corvallis, OR 97333 USA. RP Novak, JM (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 West Lucas St, Florence, SC 29501 USA. EM jeff.novak@ars.usda.gov FU US Department of Agriculture-Agricultural Research Service [60-6657-1-204]; US Environmental Protection Agency (EPA) [DE-12-92342301-1] FX The information in this article has been funded through an Interagency Agreement between the US Department of Agriculture-Agricultural Research Service (60-6657-1-204) and the US Environmental Protection Agency (EPA; DE-12-92342301-1). It has been subject to review by scientists of the USDA-ARS Coastal Plain Research Laboratory and by the National Health and Environment Effects Research Laboratory's Western Ecology Division and approved for journal submission. Approval does not signify that the contents reflect the views of the US EPA, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. We thank Ms. Sheeneka Green, Brittany Wallace, Cierra Buckman, Takeyah Powell, and Mr. Jerry Martin II for the laboratory assistance. We also thank Drs. Kurt Spokas, Jim Ippolito, and Saran Sohi for the lively conversation and for the scientific refinement of the designer biochar concept. NR 54 TC 31 Z9 31 U1 9 U2 90 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 EI 1614-7480 J9 J SOIL SEDIMENT JI J. Soils Sediments PD FEB PY 2014 VL 14 IS 2 BP 330 EP 343 DI 10.1007/s11368-013-0680-8 PG 14 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA AA3AN UT WOS:000330965400010 ER PT J AU Liu, CK Latona, NP Taylor, MM AF Liu, Cheng-Kung Latona, Nicholas P. Taylor, Maryann M. TI PREPARATIONS OF NONWOVEN AND GREEN COMPOSITES FROM COLLAGEN FIBROUS NETWORKS SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID UN-TANNED HIDES; CROSS-LINKING AB The disposal of solid wastes, such as trimmings and splits generated in various manufacturing processes in a tannery is a serious challenge to the hides and leather industries. Most of these wastes are transported out of processing plants for landfills, not only incurring the expense of transportation, but also creating environmental issues. Our effort to address these new challenges is to develop new uses and novel biobased products from solid wastes to improve prospective markets for the hides and leather industries. We hypothesize collagen fiber networks derived from solid fibrous wastes can be utilized to prepare high performance green composites and air filters, of which both have a great market potential. Collagen fiber networks were obtained from split hides that have been processed to remove the noncollagenous materials through the hair removal, liming, and bating steps. Earlier studies were devoted to understand the effects of dehydration on the resultant fiber networks and the effects of processing steps such as bating, pickling and crosslinking treatments on the morphology and physical properties of the fiber networks derived from un-tanned hides, which will be the starting material for constructing air filters and green composites. This study focuses on preparations of nonwoven and green composites derived from fiber networks. Non-woven sheets were prepared using paper-making technology. They were then used as reinforced components to make composites that use gelatin as the matrix. Mechanical properties were evaluated for the resultant composites; results showed that the fiber sizes and gelatin content have significant effects on the properties of the resultant nonwon and composites. C1 [Liu, Cheng-Kung; Latona, Nicholas P.; Taylor, Maryann M.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Liu, CK (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM ChengKung.Liu@ars.usda.gov NR 9 TC 4 Z9 4 U1 3 U2 10 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD FEB PY 2014 VL 109 IS 2 BP 35 EP 40 PG 6 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA AA3RJ UT WOS:000331009700001 ER PT J AU Kippes, N Zhu, J Chen, A Vanzetti, L Lukaszewski, A Nishida, H Kato, K Dvorak, J Dubcovsky, J AF Kippes, Nestor Zhu, Jie Chen, Andrew Vanzetti, Leonardo Lukaszewski, Adam Nishida, Hidetaka Kato, Kenji Dvorak, Jan Dubcovsky, Jorge TI Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat SO MOLECULAR GENETICS AND GENOMICS LA English DT Article DE Wheat; Vernalization; VRN-D4; VRN1; Genetic mapping; VIL; Flowering ID POLYPLOID WHEAT; TETRAPLOID WHEAT; GROWTH HABIT; MOLECULAR CHARACTERIZATION; BRACHYPODIUM-DISTACHYON; GEOGRAPHICAL VARIATION; GENOME PROGENITOR; AEGILOPS-TAUSCHII; DRAFT GENOME; BARLEY AB Wheat vernalization requirement is mainly controlled by the VRN1, VRN2, VRN3, and VRN4 genes. The first three have been cloned and have homoeologs in all three genomes. VRN4 has been found only in the D genome (VRN-D4) and has not been cloned. We constructed a high-density genetic map of the VRN-D4 region and mapped VRN-D4 within a 0.09 cM interval in the centromeric region of chromosome 5D. Using telocentric 5D chromosomes generated from the VRN-D4 donor Triple Dirk F, we determined that VRN-D4 is located on the short arm. The VRN-D4 candidate region is colinear with a 2.24 Mb region on Brachypodium distachyon chromosome 4, which includes 127 predicted genes. Ten of these genes have predicted roles in development but we detected no functional polymorphisms associated to VRN-D4. Two recombination events separated VRN-D4 from TaVIL-D1, the wheat homolog of Arabidopsis vernalization gene VIL1, confirming that this gene is not a candidate for VRN-D4. We detected significant interactions between VRN-D4 and other four genes controlling vernalization requirement (Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3), which confirmed that VRN-D4 is part of the vernalization pathway and that it is either upstream or is part of the regulatory feedback loop involving VRN1, VRN2 and VRN3 genes. The precise mapping of VRN-D4 and the characterization of its interactions with other vernalization genes provide valuable information for the utilization of VRN-D4 in wheat improvement and for our current efforts to clone this vernalization gene. C1 [Kippes, Nestor; Chen, Andrew; Dvorak, Jan; Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Zhu, Jie] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Vanzetti, Leonardo] INTA EEA Marcos Juarez, Grp Biotecnol & Rec Genet, RA-2580 Cordoba, Argentina. [Vanzetti, Leonardo] Consejo Nacl Invest Cient & Tecn CONICET, Buenos Aires, DF, Argentina. [Lukaszewski, Adam] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Nishida, Hidetaka; Kato, Kenji] Okayama Univ, Grad Sch Environm & Life Sci, Kita Ku, Okayama 7008530, Japan. [Dubcovsky, Jorge] Howard Hughes Med Inst, Chevy Chase, MD USA. [Dubcovsky, Jorge] Gordon & Betty Moore Fdn, Chevy Chase, MD USA. RP Dubcovsky, J (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM jdubcovsky@ucdavis.edu RI Dubcovsky, Jorge/A-4969-2008; OI Dubcovsky, Jorge/0000-0002-7571-4345; Kippes, Nestor/0000-0002-5852-1963 FU National Research Initiative from the USDA National Institute of Food and Agriculture [2011-67013-30077, 2011-68002-30029]; Howard Hughes Medical Institute; Gordon and Betty Moore Foundation [GBMF3031]; Japan Society for the Promotion of Science (JSPS) [23780005] FX This project was supported by the National Research Initiative (Grants 2011-67013-30077 and 2011-68002-30029) from the USDA National Institute of Food and Agriculture, by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation grant GBMF3031, and Grant-in-Aids for Young Scientists (B) (23780005) from Japan Society for the Promotion of Science (JSPS). We are grateful to Dr. Takashi Matsumoto at NIAS in Tsukuba, Japan, for screening Chinese Spring BAC library and providing a positive clone including TaVIL-D1. NR 49 TC 15 Z9 18 U1 3 U2 52 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-4615 EI 1617-4623 J9 MOL GENET GENOMICS JI Mol. Genet. Genomics PD FEB PY 2014 VL 289 IS 1 BP 47 EP 62 DI 10.1007/s00438-013-0788-y PG 16 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA AA3NL UT WOS:000330999300005 PM 24213553 ER PT J AU Sundaram, ME Meydani, SN Vandermause, M Shay, DK Coleman, LA AF Sundaram, Maria E. Meydani, Simin Nikbin Vandermause, Mary Shay, David K. Coleman, Laura A. TI Vitamin E, vitamin A, and zinc status are not related to serologic response to influenza vaccine in older adults: an observational prospective cohort study SO NUTRITION RESEARCH LA English DT Article DE Influenza; Vaccine; Vitamin A; Vitamin E; Zinc; Immune response; Observational prospective cohort study ID RANDOMIZED CONTROLLED-TRIAL; IMMUNE-RESPONSE; T-CELLS; MICRONUTRIENT DEFICIENCIES; E SUPPLEMENTATION; ALPHA-TOCOPHEROL; ELDERLY SUBJECTS; ASSOCIATION; PLASMA; IMPACT AB It has been hypothesized that micronutrient levels play a role in the immune response to vaccination; however, population-level research on the association between micronutrient levels and immune response to influenza vaccination is needed. In this study, we hypothesized that decreasing levels of nutrients would be associated with decreased hemagglutination inhibition (HAI) responses to influenza vaccination. Therefore, the purpose of this study was to determine whether serum vitamin A, vitamin E, or zinc levels are associated with influenza vaccine response determined by HAI titer in adults 65 years or older. Participants in this study included 205 community-dwelling adults 65 years or older who resided in Marshfield, WI, USA, from fall 2008 through spring 2009. Participants received trivalent influenza vaccine and donated blood samples before and 21 to 28 days after vaccination. Prevaccination levels of serum retinol, a-tocopherol, and zinc as well as prevaccination and postvaccination HAI titer levels were measured. No participants were vitamin A or vitamin E deficient; 20% had low serum zinc levels (<70 mu g/dL). Continuous variables and categorical quartiles coding for vitamin A, vitamin E, and zinc levels were not related to prevaccination or postvaccination seroprotection or seroconversion for any of the vaccine components (influenza A [H1N1], A [H3N2], or B), after adjusting for age, sex, body mass index, and prevaccination HAI geometric mean titer. In conclusion, our study population showed no association between variations in levels of serum vitamin A, vitamin E, or zinc and influenza vaccine response as measured by HAI in adults older than 65 years. Thus, associations between micronutrients and other measures of vaccine response, such as cell-mediated immune parameters, should also be explored. (C) 2014 Elsevier Inc. All rights reserved. C1 [Sundaram, Maria E.; Vandermause, Mary; Coleman, Laura A.] Marshfield Clin Res Fdn, Marshfield, WI USA. [Meydani, Simin Nikbin] Tufts Univ, Nutr Immunol Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Shay, David K.] Ctr Dis Control & Prevent, Influenza Div, Atlanta, GA USA. [Coleman, Laura A.] Abbott Nutr Res & Dev, Columbus, OH USA. RP Coleman, LA (reprint author), 3300 Stelzer Ave, Columbus, OH 43215 USA. EM laura.coleman@abbott.com OI Shay, David/0000-0001-9619-4820 FU MedImmune, LLC.; Centers for Disease Control and Prevention [5 U18 IP000183-02] FX The authors gratefully acknowledge the work of research coordinators, interviewers, and study managers at Marshfield Clinic as well as reviewers at the Centers for Disease Control and Prevention, without whom this research would not have been possible. S. N. Meydani, M. Vandermause, and D. K. Shay have no conflicts of interest. L.A. Coleman is an employee of Abbott Nutrition; however, at the time this research was completed, L.A. Coleman was an employee of Marshfield Clinic Research Foundation. M.E. Sundaram receives research funding from MedImmune, LLC. This project was supported by Centers for Disease Control and Prevention Grant 5 U18 IP000183-02. NR 40 TC 1 Z9 1 U1 1 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD FEB PY 2014 VL 34 IS 2 BP 149 EP 154 DI 10.1016/j.nutres.2013.12.004 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AA2KV UT WOS:000330924400007 PM 24461316 ER PT J AU Mithal, A Bonjour, JP Dawson-Hughes, B AF Mithal, A. Bonjour, J. -P. Dawson-Hughes, B. CA IOF CSA Nutr Working Grp TI Impact of nutrition on muscle mass, strength, and performance in older adults: response to Scott and Jones SO OSTEOPOROSIS INTERNATIONAL LA English DT Letter C1 [Mithal, A.] Medanta Medic, Gurgaon, Haryana, India. [Bonjour, J. -P.] Univ Hosp, Div Bone Dis, Geneva, Switzerland. [Bonjour, J. -P.] Fac Med, Geneva, Switzerland. [Dawson-Hughes, B.] Tufts Univ, USDA, Nutr Ctr, Medford, MA 02155 USA. RP Mithal, A (reprint author), Medanta Medic, Gurgaon, Haryana, India. EM ambrishmithal@hotmail.com NR 1 TC 2 Z9 2 U1 0 U2 1 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD FEB PY 2014 VL 25 IS 2 BP 793 EP 793 DI 10.1007/s00198-013-2490-7 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA AA3GO UT WOS:000330981100049 PM 24057479 ER PT J AU Campbell, MS Law, MY Holt, C Stein, JC Moghe, GD Hufnagel, DE Lei, JK Achawanantakun, R Jiao, D Lawrence, CJ Ware, D Shiu, SH Childs, KL Sun, YN Jiang, N Yandell, M AF Campbell, Michael S. Law, MeiYee Holt, Carson Stein, Joshua C. Moghe, Gaurav D. Hufnagel, David E. Lei, Jikai Achawanantakun, Rujira Jiao, Dian Lawrence, Carolyn J. Ware, Doreen Shiu, Shin-Han Childs, Kevin L. Sun, Yanni Jiang, Ning Yandell, Mark TI MAKER-P: A Tool Kit for the Rapid Creation, Management, and Quality Control of Plant Genome Annotations SO PLANT PHYSIOLOGY LA English DT Article ID LINEAGE-SPECIFIC GENES; ARABIDOPSIS-THALIANA; TRANSPOSABLE ELEMENTS; LTR RETROTRANSPOSONS; EUKARYOTIC GENOME; PROVIDES INSIGHTS; SEQUENCE; IDENTIFICATION; EVOLUTION; DATABASE AB We have optimized and extended the widely used annotation engine MAKER in order to better support plant genome annotation efforts. New features include better parallelization for large repeat-rich plant genomes, noncoding RNA annotation capabilities, and support for pseudogene identification. We have benchmarked the resulting software tool kit, MAKER-P, using the Arabidopsis (Arabidopsis thaliana) and maize (Zea mays) genomes. Here, we demonstrate the ability of the MAKER-P tool kit to automatically update, extend, and revise the Arabidopsis annotations in light of newly available data and to annotate pseudogenes and noncoding RNAs absent from The Arabidopsis Informatics Resource 10 build. Our results demonstrate that MAKER-P can be used to manage and improve the annotations of even Arabidopsis, perhaps the best-annotated plant genome. We have also installed and benchmarked MAKER-P on the Texas Advanced Computing Center. We show that this public resource can de novo annotate the entire Arabidopsis and maize genomes in less than 3 h and produce annotations of comparable quality to those of the current The Arabidopsis Information Resource 10 and maize V2 annotation builds. C1 [Campbell, Michael S.; Law, MeiYee; Yandell, Mark] Univ Utah, Eccles Inst Human Genet, Salt Lake City, UT 84112 USA. [Law, MeiYee] Univ Utah, Dept Biomed Informat, Salt Lake City, UT 84112 USA. [Holt, Carson] Ontario Inst Canc Res, Toronto, ON M5G 1L7, Canada. [Moghe, Gaurav D.; Shiu, Shin-Han; Jiang, Ning] Michigan State Univ, Genet Program, E Lansing, MI 48824 USA. [Hufnagel, David E.; Shiu, Shin-Han; Childs, Kevin L.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Achawanantakun, Rujira; Sun, Yanni] Michigan State Univ, Dept Comp Sci & Engn, E Lansing, MI 48824 USA. [Stein, Joshua C.; Jiang, Ning] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Jiao, Dian] Univ Texas Austin, Texas Adv Comp Ctr, Austin, TX 78758 USA. [Lawrence, Carolyn J.] Iowa State Univ, ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Lawrence, Carolyn J.] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. [Ware, Doreen] IPlant Collaborat, Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. [Ware, Doreen] ARS, USDA, North Atlantic Area, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Yandell, M (reprint author), Univ Utah, Eccles Inst Human Genet, Salt Lake City, UT 84112 USA. EM myandell@genetics.utah.edu RI Lawrence, Carolyn/N-3367-2014; LEI, JIKAI/A-2047-2015; Jiang, Ning/G-6546-2012; Childs, Kevin/C-9513-2014; OI Lawrence, Carolyn/0000-0003-0069-1430; Childs, Kevin/0000-0002-3680-062X; Lei, Jikai/0000-0002-6081-2276; Moghe, Gaurav/0000-0002-8761-064X; Shiu, Shin-Han/0000-0001-6470-235X FU National Science Foundation [IOS-1126998]; iPlant Collaborative [DBI-0735191] FX This work was supported by the National Science Foundation (grant no. IOS-1126998 to S.-H.S., K.L.C., Y.S., N.J., and M.Y. and grant no. DBI-0735191 to the iPlant Collaborative). NR 62 TC 41 Z9 41 U1 2 U2 26 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD FEB PY 2014 VL 164 IS 2 BP 513 EP 524 DI 10.1104/pp.113.230144 PG 12 WC Plant Sciences SC Plant Sciences GA AA5JN UT WOS:000331132300002 PM 24306534 ER PT J AU Prikhodko, VG Sandoval-Jaime, C Abente, EJ Bok, K Parra, GI Rogozin, IB Ostlund, EN Green, KY Sosnovtsev, SV AF Prikhodko, Victor G. Sandoval-Jaime, Carlos Abente, Eugenio J. Bok, Karin Parra, Gabriel I. Rogozin, Igor B. Ostlund, Eileen N. Green, Kim Y. Sosnovtsev, Stanislav V. TI Genetic characterization of feline calicivirus strains associated with varying disease manifestations during an outbreak season in Missouri (1995-1996) SO VIRUS GENES LA English DT Article DE Caliciviridae; Feline calicivirus; Novel strains; Complete genome; FCV phylogeny ID CAPSID PROTEIN GENE; NUCLEOTIDE-SEQUENCE; PHYLOGENETIC ANALYSIS; HYPERVARIABLE REGION; STRUCTURAL INSIGHTS; RESPIRATORY-TRACT; CARRIER STATE; IN-VITRO; CATS; VIRUS AB Feline calicivirus (FCV) is a common cause of mild to severe upper respiratory tract disease (URTD) in cats. FCV strain 21223 was isolated from a kitten with severe pneumonia in a disease outbreak with unusually high mortality (35 %) that occurred in a Missouri feline colony in 1995-1996. Phylogenetic analysis of the genome sequence of strain 21223 indicated the emergence of a new FCV strain. Analysis of the full-length genome sequence of a closely related (99.5 % nucleotide identity) strain, 3786, obtained from an asymptomatic animal in the same colony four months later, showed the presence of seven amino acid substitutions, with six of them located in the VP1 capsid sequence encoded by ORF2. Comparative analysis of the E-region sequences (426-521 aa ORF2) presumably involved in virus-host cell receptor interactions did not identify amino acid substitutions unique to the virulent strain. We determined the complete genome sequences of four virus isolates that were collected in regional catteries in the months following the outbreak that were associated with different manifestations of the disease (URTD, chronic stomatitis, and gingivitis). We show that genetically distinct FCV strains were cocirculating in the area, and no apparent correlation could be made between overall sequence and observed disease. C1 [Prikhodko, Victor G.; Sandoval-Jaime, Carlos; Abente, Eugenio J.; Bok, Karin; Parra, Gabriel I.; Green, Kim Y.; Sosnovtsev, Stanislav V.] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. [Rogozin, Igor B.] NIH, Natl Ctr Biotechnol Informat, NLM, Bethesda, MD 20894 USA. [Ostlund, Eileen N.] Anim & Plant Hlth Inspect Serv, Diagnost Virol Lab, Natl Vet Serv Labs, USDA, Ames, IA 50010 USA. RP Sosnovtsev, SV (reprint author), NIAID, Infect Dis Lab, NIH, 50 South Dr MSC8007,Bldg 50,Room 6316, Bethesda, MD 20892 USA. EM ssosnovtse@niaid.nih.gov OI Abente, Eugenio/0000-0002-3390-2786 FU NIH, NIAID FX This research was supported by the Intramural Research Program of the NIH, NIAID. NR 71 TC 5 Z9 6 U1 1 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 EI 1572-994X J9 VIRUS GENES JI Virus Genes PD FEB PY 2014 VL 48 IS 1 BP 96 EP 110 DI 10.1007/s11262-013-1005-0 PG 15 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA AA6LZ UT WOS:000331211100012 PM 24217871 ER PT J AU Wiens, JD Anthony, RG Forsman, ED AF Wiens, J. David Anthony, Robert G. Forsman, Eric D. TI Competitive Interactions and Resource Partitioning Between Northern Spotted Owls and Barred Owls in Western Oregon SO WILDLIFE MONOGRAPHS LA English DT Review DE barred owl; competition; home range; niche overlap; northern spotted owl; reproduction; resource partitioning; Strix occidentalis caurina; Strix varia; survival ID SITE OCCUPANCY DYNAMICS; HOME-RANGE; HABITAT SELECTION; INTERSPECIFIC COMPETITION; NICHE OVERLAP; STRIX-VARIA; DEMOGRAPHIC PERFORMANCE; REPRODUCTIVE SUCCESS; POPULATION ECOLOGY; CASCADE MOUNTAINS AB The federally threatened northern spotted owl (Strix occidentalis caurina) is the focus of intensive conservation efforts that have led to much forested land being reserved as habitat for the owl and associated wildlife species throughout the Pacific Northwest of the United States. Recently, however, a relatively new threat to spotted owls has emerged in the form of an invasive competitor: the congeneric barred owl (S. varia). As barred owls have rapidly expanded their populations into the entire range of the northern spotted owl, mounting evidence indicates that they are displacing, hybridizing with, and even killing spotted owls. The range expansion by barred owls into western North America has made an already complex conservation issue even more contentious, and a lack of information on the ecological relationships between the 2 species has hampered recovery efforts for northern spotted owls. We investigated spatial relationships, habitat use, diets, survival, and reproduction of sympatric spotted owls and barred owls in western Oregon, USA, during 2007-2009. Our overall objective was to determine the potential for and possible consequences of competition for space, habitat, and food between these previously allopatric owl species. Our study included 29 spotted owls and 28 barred owls that were radio-marked in 36 neighboring territories and monitored over a 24-month period. Based on repeated surveys of both species, the number of territories occupied by pairs of barred owls in the 745-km(2) study area (82) greatly outnumbered those occupied by pairs of spotted owls (15). Estimates of mean size of home ranges and core-use areas of spotted owls (1,843ha and 305ha, respectively) were 2-4 times larger than those of barred owls (581ha and 188ha, respectively). Individual spotted and barred owls in adjacent territories often had overlapping home ranges, but interspecific space sharing was largely restricted to broader foraging areas in the home range with minimal spatial overlap among core-use areas. We used an information-theoretic approach to rank discrete-choice models representing alternative hypotheses about the influence of forest conditions, topography, and interspecific interactions on species-specific patterns of nighttime resource selection. Spotted owls spent a disproportionate amount of time foraging on steep slopes in ravines dominated by old (>120 yr) conifer trees. Barred owls used available forest types more evenly than spotted owls, and were most strongly associated with patches of large hardwood and conifer trees that occupied relatively flat areas along streams. Spotted and barred owls differed in the relative use of old conifer forest (greater for spotted owls) and slope conditions (steeper slopes for spotted owls), but we found no evidence that the 2 species differed in their use of young, mature, and riparian-hardwood forest types. Mean overlap in proportional use of different forest types between individual spotted owls and barred owls in adjacent territories was 81% (range=30-99%). The best model of habitat use for spotted owls indicated that the relative probability of a location being used was substantially reduced if the location was within or in close proximity to a core-use area of a barred owl. We used pellet analysis and measures of food-niche overlap to determine the potential for dietary competition between spatially associated pairs of spotted owls and barred owls. We identified 1,223 prey items from 15 territories occupied by spotted owls and 4,299 prey items from 24 territories occupied by barred owls. Diets of both species were dominated by nocturnal mammals, but diets of barred owls included many terrestrial, aquatic, and diurnal prey species that were rare or absent in diets of spotted owls. Northern flying squirrels (Glaucomys sabrinus), woodrats (Neotoma fuscipes, N. cinerea), and lagomorphs (Lepus americanus, Sylvilagus bachmani) were primary prey for both owl species, accounting for 81% and 49% of total dietary biomass for spotted owls and barred owls, respectively. Mean dietary overlap between pairs of spotted and barred owls in adjacent territories was moderate (42%; range=28-70%). Barred owls displayed demographic superiority over spotted owls; annual survival probability of spotted owls from known-fate analyses (0.81, SE=0.05) was lower than that of barred owls (0.92, SE=0.04), and pairs of barred owls produced an average of 4.4 times more young than pairs of spotted owls over a 3-year period. We found a strong, positive relationship between seasonal (6-month) survival probabilities of both species and the proportion of old (>120 yr) conifer forest within individual home ranges, which suggested that availability of old forest was a potential limiting factor in the competitive relationship between these 2 species. The annual number of young produced by spotted owls increased linearly with increasing distance from a territory center of a pair of barred owls, and all spotted owls that attempted to nest within 1.5km of a nest used by barred owls failed to successfully produce young. We identified strong associations between the presence of barred owls and the behavior and fitness potential of spotted owls, as shown by changes in movements, habitat use, and reproductive output of spotted owls exposed to different levels of spatial overlap with territorial barred owls. When viewed collectively, our results support the hypothesis that interference competition with barred owls for territorial space can constrain the availability of critical resources required for successful recruitment and reproduction of spotted owls. Availability of old forests and associated prey species appeared to be the most strongly limiting factors in the competitive relationship between these species, indicating that further loss of these conditions can lead to increases in competitive pressure. Our findings have broad implications for the conservation of spotted owls, as they suggest that spatial heterogeneity in vital rates may not arise solely because of differences among territories in the quality or abundance of forest habitat, but also because of the spatial distribution of a newly established competitor. Experimental removal of barred owls could be used to test this hypothesis and determine whether localized control of barred owl numbers is an ecologically practical and socio-politically acceptable management tool to consider in conservation strategies for spotted owls. (c) 2013 The Wildlife Society C1 [Wiens, J. David] Oregon State Univ, US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Dept Fisheries & Wildlife,Oregon Cooperat Fish &, Corvallis, OR 97331 USA. [Anthony, Robert G.] Oregon State Univ, Dept Fisheries & Wildlife, Oregon Cooperat Fish & Wildlife Res Unit, Corvallis, OR 97331 USA. [Forsman, Eric D.] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Corvallis, OR 97331 USA. RP Wiens, JD (reprint author), Oregon State Univ, US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Dept Fisheries & Wildlife,Oregon Cooperat Fish &, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM jwiens@usgs.gov NR 206 TC 16 Z9 16 U1 24 U2 213 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0084-0173 EI 1938-5455 J9 WILDLIFE MONOGR JI Wildl. Monogr. PD FEB PY 2014 VL 185 IS 1 BP 1 EP 50 DI 10.1002/wmon.1009 PG 50 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AB3OM UT WOS:000331700100001 ER PT J AU Berthiller, F Burdaspal, PA Crews, C Iha, MH Krska, R Lattanzio, VMT MacDonald, S Malone, RJ Maragos, C Solfrizzo, M Stroka, J Whitaker, TB AF Berthiller, F. Burdaspal, P. A. Crews, C. Iha, M. H. Krska, R. Lattanzio, V. M. T. MacDonald, S. Malone, R. J. Maragos, C. Solfrizzo, M. Stroka, J. Whitaker, T. B. TI Developments in mycotoxin analysis: an update for 2012-2013 SO WORLD MYCOTOXIN JOURNAL LA English DT Review DE aflatoxin; Alternaria toxins; ergot alkaloids; fumonisin; ochratoxin; patulin; trichothecene; zearalenone; sampling; multimycotoxin; botanicals; method development ID TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; IMMUNOAFFINITY COLUMN CLEANUP; LC-MS/MS METHOD; CAPILLARY-ZONE-ELECTROPHORESIS; ALTERNARIOL MONOMETHYL ETHER; LINKED-IMMUNOSORBENT-ASSAY; IN-HOUSE VALIDATION; CEREAL-BASED FOODS AB This review highlights developments in mycotoxin analysis and sampling over a period between mid-2012 and mid-2013. It covers the major mycotoxins: aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxins, patulin, trichothecenes and zearalenone. A wide range of analytical methods for mycotoxin determination in food and feed were developed last year, in particular immunochemical methods and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)-based methods. After a section on sampling and sample preparation, due to the rapid spread and developments in the field of LC-MS/MS multimycotoxin methods, a separate section has been devoted to this area of research. It is followed by a section on mycotoxins in botanicals and spices, before continuing with the format of previous reviews in this series with dedicated sections on method developments for the individual mycotoxins. C1 [Berthiller, F.; Krska, R.] Univ Nat Resources & Life Sci, Dept Agrobiotechnol IFA Tulln, Christian Doppler Lab Mycotoxin Metab, A-3430 Tulln, Austria. [Berthiller, F.; Krska, R.] Univ Nat Resources & Life Sci, Ctr Analyt Chem, A-3430 Tulln, Austria. [Burdaspal, P. A.] Spanish Food Safety & Nutr Agcy, Natl Food Ctr, Majadahonda 228220, Spain. [Crews, C.; MacDonald, S.] Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England. [Iha, M. H.] Adolfo Lutz Inst, Lab Ribeiro Preto 1, BR-14085410 Ribeirao Preto, SP, Brazil. [Lattanzio, V. M. T.; Solfrizzo, M.] CNR, Inst Sci Food Prod, I-700126 Bari, Italy. [Malone, R. J.] Tril Analyt Lab, Washington, MO 63090 USA. [Maragos, C.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Stroka, J.] European Commiss, Joint Res Ctr, Inst Reference Mat & Measurements, B-2440 Geel, Belgium. [Whitaker, T. B.] N Carolina State Univ, Dept Biol & Agr Engn, Raleigh, NC 27695 USA. RP Berthiller, F (reprint author), Univ Nat Resources & Life Sci, Dept Agrobiotechnol IFA Tulln, Christian Doppler Lab Mycotoxin Metab, Konrad Lorenz Str 20, A-3430 Tulln, Austria. EM franz.berthiller@boku.ac.at NR 236 TC 31 Z9 31 U1 16 U2 161 PU WAGENINGEN ACADEMIC PUBLISHERS PI WAGENINGEN PA PO BOX 220, WAGENINGEN, 6700 AE, NETHERLANDS SN 1875-0710 EI 1875-0796 J9 WORLD MYCOTOXIN J JI World Mycotoxin J. PD FEB PY 2014 VL 7 IS 1 BP 3 EP 33 DI 10.3920/WMJ2013.1637 PG 31 WC Food Science & Technology; Mycology; Toxicology SC Food Science & Technology; Mycology; Toxicology GA AA1EF UT WOS:000330838500002 ER PT J AU Miller, JD Schaafsma, AW Bhatnagar, D Bondy, G Carbone, I Harris, LJ Harrison, G Munkvold, GP Oswald, IP Pestka, JJ Sharpe, L Sumarah, MW Tittlemier, SA Zhou, T AF Miller, J. D. Schaafsma, A. W. Bhatnagar, D. Bondy, G. Carbone, I. Harris, L. J. Harrison, G. Munkvold, G. P. Oswald, I. P. Pestka, J. J. Sharpe, L. Sumarah, M. W. Tittlemier, S. A. Zhou, T. TI Mycotoxins that affect the North American agri-food sector: state of the art and directions for the future SO WORLD MYCOTOXIN JOURNAL LA English DT Review DE mycotoxins; USA; Canada; toxicology; mycology; crop production ID FUSARIUM HEAD BLIGHT; TRICHOTHECENE DEOXYNIVALENOL VOMITOXIN; BT-CORN HYBRIDS; ASPERGILLUS-FLAVUS; AFLATOXIN B-1; FUMONISIN B-1; EAR ROT; ENVIRONMENTAL-FACTORS; BIOLOGICAL-CONTROL; HUMAN EXPOSURE AB This paper summarises workshop discussions at the 5th international MYCORED meeting in Ottawa, Canada (June 2012) with over 200 participants representing academics, government and industry scientists, government officials and farming organisations (present in roughly equal proportions) from 27 countries. Workshops centred on how mycotoxins in food and feed affect value chains and trade in the region covered by the North American Free Trade Agreement. Crops are contaminated by one or more of five important mycotoxins in parts of Canada and the United States every year, and when contaminated food and feed are consumed in amounts above tolerable limits, human and animal health are at risk. Economic loss from such contamination includes reduced crop yield, grain quality, animal productivity and loss of domestic and export markets. A systematic effort by grain producers, primary, transfer, and terminal elevators, millers and food and feed processers is required to manage these contaminants along the value chain. Workshops discussed lessons learned from investments in plant genetics, fungal genomics, toxicology, analytical and sampling science, management strategies along the food and feed value chains and methods to ameliorate the effects of toxins in grain on animal production and on reducing the impact of mycotoxins on population health in developing countries. These discussions were used to develop a set of priorities and recommendations. C1 [Miller, J. D.] Carleton Univ, Dept Chem, Ottawa, ON K1S 5B6, Canada. [Schaafsma, A. W.] Univ Guelph, Ridgetown, ON N0P 2C0, Canada. [Bhatnagar, D.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Bondy, G.] Hlth Canada, Food Directorate, Bur Chem Safety, Ottawa, ON K1A 0K9, Canada. [Carbone, I.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27606 USA. [Harris, L. J.] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada. [Harrison, G.] Canadian Natl Millers Assoc, Ottawa, ON K2P 1R3, Canada. [Munkvold, G. P.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Oswald, I. P.] INRA, UMR1331, Res Ctr Food Toxicol, Toxalim, F-31027 Toulouse, France. [Pestka, J. J.] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA. [Sharpe, L.] DuPont Pioneer Hi Bred, Chatham, ON N7M 5L1, Canada. [Sumarah, M. W.] Agr & Agri Food Canada, Southern Crop Protect & Food Res Ctr, London, ON N5V 4T3, Canada. [Tittlemier, S. A.] Canadian Grain Commiss, Grain Res Lab, Winnipeg, MB R3C 3G8, Canada. [Zhou, T.] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada. RP Miller, JD (reprint author), Carleton Univ, Dept Chem, 228 Steacie Bldg, Ottawa, ON K1S 5B6, Canada. EM david.miller@carleton.ca OI OSWALD, Isabelle/0000-0001-9918-277X; Sumarah, Mark/0000-0003-0977-1656 NR 105 TC 15 Z9 15 U1 4 U2 51 PU WAGENINGEN ACADEMIC PUBLISHERS PI WAGENINGEN PA PO BOX 220, WAGENINGEN, 6700 AE, NETHERLANDS SN 1875-0710 EI 1875-0796 J9 WORLD MYCOTOXIN J JI World Mycotoxin J. PD FEB PY 2014 VL 7 IS 1 BP 63 EP 82 DI 10.3920/WMJ2013.1624 PG 20 WC Food Science & Technology; Mycology; Toxicology SC Food Science & Technology; Mycology; Toxicology GA AA1EF UT WOS:000330838500006 ER PT J AU Dorado, C Mullen, CA Boateng, AA AF Dorado, Christina Mullen, Charles A. Boateng, Akwasi A. TI H-ZSM5 Catalyzed Co-Pyrolysis of Biomass and Plastics SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Catalytic fast pyrolysis; Agriculture; Olefin; Aromatic; Biomass ID HIGH-DENSITY POLYETHYLENE; FLUIDIZED-BED; WASTE; AROMATICS; PRODUCTS; GASOLINE; RECOVERY; CRACKING; OLEFINS; REACTOR AB This study aims to address two important problems vital to agriculture: disposal of agricultural plastics and production of drop-in fuels from biomass via co-pyrolysis of both feedstocks. Mixtures of biomass (switchgrass, cellulose, xylan, and lignin) and plastic (polyethylene terephthalate (PET), polypropylene (PP), high density polyethylene (HDPE), low density polyethylene (LDPE), and polystyrene (PS)) were subjected to catalytic fast pyrolysis (CFP) at 650 C in the presence of H-ZSM5. A micro-pyrolyzer coupled with GC/MS (py-GC/MS) was utilized to evaluate desired mixes and product distribution. Yields of the aromatic compounds typically observed as catalytic pyrolysis products including toluene, ethylbenzene, p-xylene, o-xylene, naphthalene, and 2-methylnaphthalene and their sum were tracked. The carbon yield of products from CFP of mixtures of biomass and plastic were compared with that of biomass alone, plastic alone, and the calculated arithmetic sum of yield values expected if there was no chemical interaction between the two feedstocks. The latter provides insights as to whether a synergetic effect occurred that enhanced the selectivity to aromatics of the blend or just the additive sum of the individual products. It was found that in several cases conversion enhancement occurred for the mixture, with the aliphatic polymers (PE and PP) and PET generally providing the biggest increase in total aromatic yields. Changes in selectivity for the production of individual aromatic compounds were also observed in the blends compared with the biomass or plastics alone. C1 [Dorado, Christina; Mullen, Charles A.; Boateng, Akwasi A.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Mullen, CA (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM charles.mullen@ars.usda.gov NR 38 TC 36 Z9 36 U1 6 U2 64 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD FEB PY 2014 VL 2 IS 2 BP 301 EP 311 DI 10.1021/sc400354g PG 11 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA AA0UH UT WOS:000330812400026 ER PT J AU Avery, PB Kumar, V Xiao, YF Powell, CA McKenzie, CL Osborne, LS AF Avery, Pasco B. Kumar, Vivek Xiao, Yingfang Powell, Charles A. McKenzie, Cindy L. Osborne, Lance S. TI Selecting an ornamental pepper banker plant for Amblyseius swirskii in floriculture crops SO ARTHROPOD-PLANT INTERACTIONS LA English DT Article DE Biological control; Tuft domatia; Scirtothrips dorsalis; Pepper banker plants; Pollen; Floriculture crops ID DORSALIS THYSANOPTERA THRIPIDAE; BIOLOGICAL-CONTROL AGENTS; PREDATORY MITES ACARI; WESTERN FLOWER THRIPS; LEAF DOMATIA; FRANKLINIELLA-OCCIDENTALIS; PHYTOSEIID PREDATORS; BEMISIA-TABACI; CHILLI THRIPS; ABUNDANCE AB Preference of phytoseiid mite, Amblyseius swirskii (Athias-Henriot) was assessed on four cultivars of ornamental pepper banker plant candidates; Red Missile (RM), Masquerade (MA), Explosive Ember (EE) and Black Pearl (BP) for potential control of pestiferous insects in floriculture. Significant differences in cultivar preference by A. swirskii was observed in choice experiments whether the test was pre- (with pollen) or during bloom. Overall, female mites laid more eggs when pollen was provided as a food source. The number of tuft domatia per cultivar leaf appeared to positively influence host preference in the choice plant tests pre-bloom. In addition, cultivar RM had the highest mean number +/- A SEM of tuft domatia per leaf (5.1 +/- A 0.3) and motiles per plant (4.0 +/- A 1.2), followed by MA, EE and BP. In choice tests on blooming plants, A. swirskii showed preference for both cultivars RM and MA compared to EE. These experiments indicated that the number of tuft domatia and availability of pollen can influence the host preference of A. swirskii for an ornamental pepper banker plant cultivar. Results from this study will help growers, researchers, educators and extension personnel in understanding the plant phenology promoting adoption of suitable banker plants for managing greenhouse and landscape insect pests. C1 [Avery, Pasco B.; Powell, Charles A.] Univ Florida, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA. [Kumar, Vivek; Xiao, Yingfang; Osborne, Lance S.] Univ Florida, Dept Entomol & Nematol, Midflorida Res & Educ Ctr, IFAS, Apopka, FL 32703 USA. [McKenzie, Cindy L.] ARS, US Hort Res Lab, Subtrop Insect Res Unit, USDA, Ft Pierce, FL 34945 USA. RP Avery, PB (reprint author), Univ Florida, Indian River Res & Educ Ctr, 2199 South Rock Rd, Ft Pierce, FL 34945 USA. EM pbavery@ufl.edu RI Kumar, Vivek/E-5777-2016 OI Kumar, Vivek/0000-0003-1988-2536 FU EPA; USDA/T-STAR; USDA-ARS Floriculture and Nursery Research Initiative; IFAS/UF Line Item grants program FX The authors would like to thank Katherine Houben at Mid-Florida Research and Education Center in Apopka, FL, for her technical assistance in the rearing of A. swirskii; John Prokop and Lynn Faulkner for their technical assistance in the greenhouse at the USDA, ARS, US Horticultural Research Laboratory (USHRL), Ft. Pierce, FL; Phyllis Hebert, Ryan Loehr and David Alden at the Indian River Research and Education Center, Ft. Pierce, FL for some aspect of technical assistance in mite and plant maintenance, destructive sampling and/or counting tuft domatia and mite life stages. Funding for this study was supported by grants from the EPA, USDA/T-STAR, the USDA-ARS Floriculture and Nursery Research Initiative, and IFAS/UF Line Item grants program. NR 42 TC 7 Z9 7 U1 2 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1872-8855 EI 1872-8847 J9 ARTHROPOD-PLANT INTE JI Arthropod-Plant Interact. PD FEB PY 2014 VL 8 IS 1 BP 49 EP 56 DI 10.1007/s11829-013-9283-y PG 8 WC Ecology; Entomology SC Environmental Sciences & Ecology; Entomology GA AA0FF UT WOS:000330770200006 ER PT J AU Lantschner, MV Villacide, JM Garnas, JR Croft, P Carnegie, AJ Liebhold, AM Corley, JC AF Victoria Lantschner, M. Villacide, Jose M. Garnas, Jeffrey R. Croft, Philip Carnegie, Angus J. Liebhold, Andrew M. Corley, Juan C. TI Temperature explains variable spread rates of the invasive woodwasp Sirex noctilio in the Southern Hemisphere SO BIOLOGICAL INVASIONS LA English DT Article DE Exotic pests; Invasion ecology; Range expansion; Landscape ecology; Climate ID BIOLOGICAL-CONTROL AGENTS; FABRICIUS HYMENOPTERA; PEST-MANAGEMENT; SIRICIDAE; ECOLOGY; AFRICA; INSECT; PLANTATIONS; CANADA; AREAS AB The frequency of introductions of non-indigenous forest insects into new habitats is increasing worldwide, often with profoundly adverse consequences on natural and plantation forest ecosystems. Understanding rates and patterns of spread of invasive forest insects is important for predicting when and where these species will expand their geographical range, with the potential to improve mitigation strategies. The woodwasp Sirex noctilio is a damaging invasive forest insect that kills numerous species of Pinus. Despite encountering highly variable eco-climatic conditions, S. noctilio has arrived and established in exotic pine forest production areas throughout the Southern Hemisphere. In this study, we compiled historical records of S. noctilio invasion to compare spread rates among eight contrasting eco-climatic regions in the Southern Hemisphere and to explore how spread rate is predicted by landscape variation in climate, habitat characteristics and anthropogenic effects. Spread rates for S. noctilio varied considerably among the invaded regions, ranging from 12 to 82 km per year. Among regions, spread rates of S. noctilio increased with increasing mean annual temperature and isothermality. We hypothesize that temperature may directly or indirectly influence S. noctilio population growth and dispersal, thereby influencing spread rates. C1 [Victoria Lantschner, M.; Villacide, Jose M.; Corley, Juan C.] INTA EEA Bariloche, Grp Ecol Poblac Insectos, RA-8400 San Carlos De Bariloche, Rio Negro, Argentina. [Garnas, Jeffrey R.] Univ Pretoria, Forestry & Agr Biotechnol Inst, Dept Zool & Entomol, ZA-0002 Pretoria, South Africa. [Croft, Philip] Inst Commercial Forestry Res, Pietermaritzburg, South Africa. [Carnegie, Angus J.] NSW Dept Primary Ind, Forest Sci Ctr, Beecroft, NSW 2119, Australia. [Liebhold, Andrew M.] US Forest Serv, No Res Stn, USDA, Morgantown, WV 26505 USA. RP Lantschner, MV (reprint author), INTA EEA Bariloche, Grp Ecol Poblac Insectos, RA-8400 San Carlos De Bariloche, Rio Negro, Argentina. EM lantschner.v@inta.gob.ar RI Carnegie, Angus/H-6153-2015; OI Carnegie, Angus/0000-0002-6854-4974; Corley, Juan/0000-0002-8032-2223; Villacide, Jose M./0000-0003-1030-0296 FU Agencia Nacional para la Promocion Cientifica y Tecnologica, Argentina [PICT-1775]; Componente Plantaciones Forestales Sustentables, BIRF LN 7520 AR, Argentina [SaFo-109]; US Forest Service International Programs FX We thank the "Direccion General de Bosques y Parques de Chubut, Argentina'' and "Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA), Argentina'' for providing data used in this paper. This work was partially supported by grants: PICT-1775 (Agencia Nacional para la Promocion Cientifica y Tecnologica, Argentina); SaFo-109 (Componente Plantaciones Forestales Sustentables, BIRF LN 7520 AR, Argentina); and the US Forest Service International Programs. M. Ramsden (HQPlantations) provided unpublished data on S. noctilio detections in Queensland. NR 67 TC 17 Z9 18 U1 1 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD FEB PY 2014 VL 16 IS 2 BP 329 EP 339 DI 10.1007/s10530-013-0521-0 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 302PZ UT WOS:000330618700008 ER PT J AU Nelson, MF Anderson, NO Casler, MD Jakubowski, AR AF Nelson, Michael F. Anderson, Neil O. Casler, Michael D. Jakubowski, Andrew R. TI Population genetic structure of N. American and European Phalaris arundinacea L. as inferred from inter-simple sequence repeat markers SO BIOLOGICAL INVASIONS LA English DT Article DE ISSRs; Phalaris arundinacea; STRUCTURE; Population structure; AMOVA ID INVASIVE PLANT-POPULATIONS; MULTILOCUS GENOTYPE DATA; REED CANARYGRASS; PHRAGMITES-AUSTRALIS; WETLAND PLANTS; NORTH-AMERICA; DIVERSITY; EVOLUTION; INFERENCE; SUCCESS AB Phalaris arundinacea L. (reed canarygrass) has become one of the most aggressive invaders of North American wetlands. P. arundinacea is native to temperate N. America, Europe, and Asia, but repeated introductions of European genotypes to N. America, recent range expansions, and the planting of forage and ornamental cultivars complicate the resolution of its demographic history. Molecular tools can help to unravel the demographic and invasion history of populations of invasive species. In this study, inter-simple sequence repeat markers were used to analyze the population genetic structure of European and N. American populations of reed canary grass as well as forage and ornamental cultivars. We found that P. arundinacea harbors a high amount of genetic diversity with most of the diversity located within, as opposed to among, populations. Cluster analyses suggested that current populations are admixtures of two formerly distinct genetic groups. C1 [Nelson, Michael F.; Anderson, Neil O.] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. [Casler, Michael D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Jakubowski, Andrew R.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. RP Anderson, NO (reprint author), Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. EM ander044@umn.edu RI Nelson, Michael/C-6760-2015 OI Nelson, Michael/0000-0002-3312-7935 NR 45 TC 6 Z9 7 U1 3 U2 32 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD FEB PY 2014 VL 16 IS 2 BP 353 EP 363 DI 10.1007/s10530-013-0525-9 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 302PZ UT WOS:000330618700010 ER PT J AU Tobin, PC Kean, JM Suckling, DM McCullough, DG Herms, DA Stringer, LD AF Tobin, Patrick C. Kean, John M. Suckling, David Maxwell McCullough, Deborah G. Herms, Daniel A. Stringer, Lloyd D. TI Determinants of successful arthropod eradication programs SO BIOLOGICAL INVASIONS LA English DT Article DE Detection; Eradication; Invasive species management; Non-native pests ID UNITED-STATES; BIOLOGICAL INVASIONS; GYPSY-MOTH; PROPAGULE PRESSURE; INSECT ERADICATION; CITIZEN SCIENCE; PATHWAY; ESTABLISHMENT; SURVEILLANCE; POPULATION AB Despite substantial increases in public awareness and biosecurity systems, introductions of non-native arthropods remain an unwelcomed consequence of escalating rates of international trade and travel. Detection of an established but unwanted non-native organism can elicit a range of responses, including implementation of an eradication program. Previous studies have reviewed the concept of eradication, but these efforts were largely descriptive and focused on selected case studies. We developed a Global Eradication and Response DAtabase ("GERDA") to facilitate an analysis of arthropod eradication programs and determine the factors that influence eradication success and failure. We compiled data from 672 arthropod eradication programs targeting 130 non-native arthropod species implemented in 91 countries between 1890 and 2010. Important components of successful eradication programs included the size of the infested area, relative detectability of the target species, method of detection, and the primary feeding guild of the target species. The outcome of eradication efforts was not determined by program costs, which were largely driven by the size of the infestation. The availability of taxon-specific control tools appeared to increase the probability of eradication success. We believe GERDA, as an online database, provides an objective repository of information that will play an invaluable role when future eradication efforts are considered. C1 [Tobin, Patrick C.] US Forest Serv, USDA, No Res Stn, Morgantown, WV 26505 USA. [Kean, John M.] AgResearch Ltd, Ruakura Res Ctr, Hamilton 3240, New Zealand. [Suckling, David Maxwell; Stringer, Lloyd D.] New Zealand Inst Plant Food Res Ltd, Christchurch 8140, New Zealand. [Suckling, David Maxwell; Stringer, Lloyd D.] Plant Biosecur Cooperat Res Ctr, Canberra, ACT, Australia. [McCullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [Herms, Daniel A.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. RP Tobin, PC (reprint author), US Forest Serv, USDA, No Res Stn, Morgantown, WV 26505 USA. EM ptobin@fs.fed.us RI Suckling, David/F-7005-2010; OI Suckling, David/0000-0001-7216-9348; Stringer, Lloyd/0000-0002-6345-0768 FU National Center for Ecological Analysis and Synthesis; NSF [EF-0553768]; University of California, Santa Barbara; State of California; USDA Forest Service, Eastern Forest Environmental Threat Assessment Center, Asheville, North Carolina; New Zealand's Better Border Biosecurity research program; Australian Government's Cooperative Research Centre FX This work was conducted as part of a working group, "Applying population ecology to strategies for eradicating invasive forest insects," supported by the National Center for Ecological Analysis and Synthesis (http://www.nceas.ucsb.edu/), a Center funded by NSF (Grant No. EF-0553768), the University of California, Santa Barbara, the State of California and the USDA Forest Service, Eastern Forest Environmental Threat Assessment Center, Asheville, North Carolina. We are very grateful to the numerous colleagues and biosecurity practitioners who assisted in the compilation of data on their respective eradication programs. We thank Laura Blackburn (USDA Forest Service) for technical assistance. We also acknowledge support from New Zealand's Better Border Biosecurity research program (b3nz.org) and an Australian Government's Cooperative Research Centre (www.pbcrc.com.au). We are grateful to three anonymous reviewers for constructive comments. NR 55 TC 26 Z9 28 U1 3 U2 58 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD FEB PY 2014 VL 16 IS 2 BP 401 EP 414 DI 10.1007/s10530-013-0529-5 PG 14 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 302PZ UT WOS:000330618700014 ER PT J AU Baez, C Considine, J Rowlands, R AF Baez, Carlos Considine, John Rowlands, Robert TI Influence of drying restraint on physical and mechanical properties of nanofibrillated cellulose films SO CELLULOSE LA English DT Article DE Nanofibrillated cellulose; Microfibrillated cellulose; Nanocellulose; Restraint drying; Fiber orientation; Cellulose nanofibers ID TEMPO-MEDIATED OXIDATION; MOLECULAR-ORIENTATION; MICROFIBRILLATED CELLULOSE; RAMAN SPECTROSCOPY; MORPHOLOGY; FIBERS; NANOPAPER AB Nanofibrillated cellulose (NFC) is a renewable and biodegradable fibril that possesses high strength and stiffness resulting from high level hydrogen bonding. Films made from NFC shrink and distort as they transition from a wet state (20 wt% solids) to a state of moisture equilibrium (90 wt% solids at 50 % RH, 23 A degrees C). Material distortions are driven by development of moisture gradients within the fibril network and effectively reduce mechanical performance. For this study, NFC was extracted from softwood holocellulose by first employing a chemical pretreatment [(2,2,6,6-tetramethylpiperidin-1-yl)oxyl catalyzed oxidation] followed by mechanical fibrillation using ultrasound energy. To assess the problem of film distortion, neat NFC films were dried at 50 % RH, 23 A degrees C under one of the following three restraint conditions: fully restrained, partially restrained, and uniaxially drawn. The influence of restraint condition on the resulting physical and mechanical properties was evaluated. Raman and X-ray results showed that fibrils in the uniaxially drawn specimens tended to align with the drawing axis, whereas no in-plane orientation effects were observed for the fully or partially restrained specimens. Fully restrained specimens had a respective strength and stiffness of 222 MPa and 14 GPa in every (in-plane) direction. However, samples that were wet-drawn to a 30 % strain level had a respective strength and stiffness of 474 MPa and 46 GPa in the direction of draw. Mechanical properties for axially drawn specimens had both fibril alignment and fibril straightening contributions. C1 [Baez, Carlos; Considine, John] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. [Rowlands, Robert] Univ Wisconsin, Madison, WI 53706 USA. RP Baez, C (reprint author), US Forest Serv, Forest Prod Lab, USDA, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM cbaez@fs.fed.us FU National Cancer Institute (NCI), National Institutes of Health (NIH) [HHSN261200800001E] FX TEM imaging work was funded in whole or in part with federal funds from the National Cancer Institute (NCI), National Institutes of Health (NIH), under Contract No. HHSN261200800001E. We thank Drs. S.E. McNeil, U. Baxa and their colleagues at NCI/NIH for carrying out the TEM. NR 31 TC 9 Z9 9 U1 1 U2 54 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD FEB PY 2014 VL 21 IS 1 BP 347 EP 356 DI 10.1007/s10570-013-0159-1 PG 10 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA AA0SF UT WOS:000330807000026 ER PT J AU Nam, S Condon, BD Foston, MB Chang, SC AF Nam, Sunghyun Condon, Brian D. Foston, Marcus B. Chang, SeChin TI Enhanced thermal and combustion resistance of cotton linked to natural inorganic salt components SO CELLULOSE LA English DT Article DE Cotton; Inorganic salt; Thermogravimetry; Microscale combustion calorimetry; NMR; Activation energy ID CELLULOSE PYROLYSIS; GAS-CHROMATOGRAPHY; VACUUM PYROLYSIS; FLAME RETARDANTS; HEATING RATE; BIOMASS; METAL; WOOD; KINETICS; FIBERS AB A comparison of the thermal decomposition and combustion characteristics of raw and scoured cottons has demonstrated a mechanistic link caused by the presence of inorganic salts in raw cotton, which enhances resistance to heat and flame. Thermogravimetry, differential thermogravimetry, and microscale combustion calorimetry were used to examine the thermal decomposition kinetics and thermal stability of cotton. During pyrolysis, both raw cotton nonwoven and woven fabrics exhibited a slower decomposition with a larger initial weight loss and produced a greater char yield, as compared to the fabrics after scouring, which removes most inorganic components from cotton. The activation energy (E (a) ) values, calculated using the Kissinger method, the Flynn-Wall-Ozawa method, and the modified Coats-Redfern method, were consistently determined to be smaller for raw cotton than for scoured cotton. The analyses of cotton fabrics heated at elevated temperatures by C-13 CP/MAS NMR and ATR-FTIR showed that trace quantities of inorganic components promoted the formations of oxygenated moieties at low temperatures and aliphatic intermediate char. In the combustion, raw cotton exhibited a much smaller heat release capacity and a smaller total heat release than scoured cotton, indicating enhanced thermal stability when the inorganic components are intact. C1 [Nam, Sunghyun; Condon, Brian D.; Chang, SeChin] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Foston, Marcus B.] Washington Univ, Dept Energy Environm & Chem Engn, St Louis, MO 63130 USA. RP Condon, BD (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM brian.condon@ars.usda.gov NR 46 TC 6 Z9 6 U1 0 U2 38 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD FEB PY 2014 VL 21 IS 1 BP 791 EP 802 DI 10.1007/s10570-013-0133-y PG 12 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA AA0SF UT WOS:000330807000064 ER PT J AU Bock, CH Graham, JH Gottwald, TR Cook, AZ Parker, PE AF Bock, Clive H. Graham, James H. Gottwald, Tim R. Cook, Amanda Z. Parker, Paul E. TI Effect of the duration of inoculum exposure on development of citrus canker symptoms on seedlings of Swingle citrumelo SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Epidemiology; Dispersal; Infection period; Integrated disease management ID AXONOPODIS PV.-CITRI; XANTHOMONAS-CAMPESTRIS; WIND-SPEED; SPREAD; BRAZIL; TREES; WINDBREAKS; INFECTION; DISPERSAL; ARGENTINA AB Citrus canker (Xanthomonas citri subsp. citri, Xcc) is one of the most serious diseases citrus in Florida, and elsewhere in the world. The disease causes yield loss and some fresh fruit trade restrictions may apply. Cultural management techniques such as windbreaks may work by not only reducing wind speed, but also reducing the period of exposure of susceptible foliage or fruit to those wind speeds that support infection from incoming inoculum. To investigate the effect of exposure period to inoculum of Xcc, seedlings of canker-susceptible Swingle citrumelo were exposed to sprayed inoculum for increasing periods at different wind speeds. The incidence and severity of citrus canker was assessed. In three experiments the incidence and severity of citrus canker most often increased with longer periods of exposure to inoculum, especially so at wind speeds of a parts per thousand yen16 m/s compared to wind speeds of a parts per thousand currency sign5 m/s (wind speed also increased disease incidence and severity). Regression analysis demonstrated relationships between period of exposure to inoculum and the percent infected leaves per plant, the number of lesions per plant, the number of lesions per infected leaf, and for the percent of infected leaves with lesions on the petioles at wind speeds of a parts per thousand yen16 m/s (R-2 = 0.16-0.72). Due to the effect of inoculum exposure period and wind speed, attempts should be made to minimize exposure of canker-susceptible citrus when wind speed is highest and inoculum is available. Windbreaks should help minimize periods of exposure to splashed inoculum in high winds. C1 [Bock, Clive H.; Graham, James H.] Univ Florida, CREC, Lake Alfred, FL 33850 USA. [Gottwald, Tim R.] ARS, USDA, USHRL, Ft Pierce, FL 34945 USA. [Cook, Amanda Z.; Parker, Paul E.] APHIS, USDA, PPQ, Moore Air Base, Edinburg, TX 78539 USA. RP Bock, CH (reprint author), ARS, USDA, SEFTNRL, 21 Dunbar Rd, Byron, GA 31008 USA. EM clive.bock@ars.usda.gov; jhgraham@ufl.edu; tim.gottwald@ars.usda.gov; amanda.cook@tx.usda.gov; paul.e.parker@aphis.usda.gov NR 33 TC 0 Z9 0 U1 2 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD FEB PY 2014 VL 138 IS 2 BP 237 EP 245 DI 10.1007/s10658-012-0150-y PG 9 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 304FH UT WOS:000330732000005 ER PT J AU Harris, G Nielson, RM Rinaldi, T Lohuis, T AF Harris, Grant Nielson, Ryan M. Rinaldi, Todd Lohuis, Thomas TI Effects of winter recreation on northern ungulates with focus on moose (Alces alces) and snowmobiles SO EUROPEAN JOURNAL OF WILDLIFE RESEARCH LA English DT Article DE Alces alces; Moose; Recreation; Resource selection function; Snowmobiles; Ungulate ID WHITE-TAILED DEER; MULE DEER; HABITAT SELECTION; NATIONAL-PARK; ENERGY EXPENDITURES; WILD REINDEER; ELK; RESPONSES; DISTURBANCE; YELLOWSTONE AB Winter recreation can displace ungulates to poor habitats, which may raise their energy expenditure and lower individual survivorship, causing population declines. Winter recreation could be benign, however, if animals habituate. Moreover, recreation creates trails. Traveling on them could reduce energy expenditure, thereby increasing ungulate survivorship and generating population benefits. Balancing recreation use with wildlife stewardship requires identifying when these effects occur. This task would be simpler if guidelines existed to inform assessments. We developed and tested such guidelines using two approaches. First, we synthesized literature describing the effects of winter recreation-motorized and nonmotorized-on northern ungulates. This synthesis enabled formulating six guidelines, while exposing two requiring further attention (ungulate habituation and displacement). Second, we tested these two guidelines and evaluated the others by quantifying the behavioral responses of moose to snowmobiles, in two areas of south-central Alaska, differing by snowmobile predictability. For each location, we modeled moose preferences during the snowmobile period using different combinations of eight variables-static (elevation and slope), biotic (habitat and cover), and anthropogenic (distance to roads, railroads, snowmobile trails, and trail density). We identified the model with the most support and used it to estimate parameter coefficients for pre- and post-recreation periods. Changes in coefficients between periods indicated snowmobile effects on moose. Overall, we produced and evaluated six guidelines describing when winter recreation is potentially detrimental to ungulates as follows: (1) when unpredictable, (2) spanning large areas, (3) long in duration, (4) large spatial footprint, (5) nonmotorized, and (6) when animals are displaced to poor quality habitats. C1 [Harris, Grant] US Forest Serv, USDA, Anchorage, AK 99503 USA. [Nielson, Ryan M.] WEST Inc, Laramie, WY 82070 USA. [Rinaldi, Todd] Alaska Dept Fish & Game, Palmer, AK 99645 USA. [Lohuis, Thomas] Alaska Dept Fish & Game, Anchorage, AK 99518 USA. RP Harris, G (reprint author), US Fish & Wildlife Serv, POB 1306, Albuquerque, NM 87103 USA. EM grant_harris@fws.gov NR 50 TC 1 Z9 1 U1 6 U2 71 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-4642 EI 1439-0574 J9 EUR J WILDLIFE RES JI Eur. J. Wildl. Res. PD FEB PY 2014 VL 60 IS 1 BP 45 EP 58 DI 10.1007/s10344-013-0749-0 PG 14 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 302GE UT WOS:000330589700005 ER PT J AU Berger, A Gschwantner, T McRoberts, RE Schadauer, K AF Berger, Ambros Gschwantner, Thomas McRoberts, Ronald E. Schadauer, Klemens TI Effects of Measurement Errors on Individual Tree Stem Volume Estimates for the Austrian National Forest Inventory SO FOREST SCIENCE LA English DT Article DE stem volume; uncertainty; measurement error; error propagation; Monte Carlo error simulation ID LASER DENDROMETERS; PRECISION; VARIANCE; ACCURACY; HEIGHT; MODEL; AREA AB National forest inventories typically estimate individual tree volumes using models that rely on measurements of predictor variables such as tree height and diameter, both of which are subject to measurement error. The aim of this study was to quantify the impacts of these measurement errors on the uncertainty of the model-based tree stem volume estimates. The impacts were investigated using two approaches: the law of propagation of error and Monte Carlo simulation. Estimates of total uncertainty also included variability associated with the model itself. Results for both approaches indicate that the relative standard deviation over plots of the volume estimates for all tree species is approximately 11%. A partition of the total uncertainty by sources indicates that error in measurement of the upper diameter makes the greatest contribution. Thus, the greatest potential for improvement in the precision of overall estimates lies in increasing the accuracy of upper diameter measurements. Although the uncertainty of individual tree stem volume estimates may be considered negligible for nationwide assessments of growing stock volume, it is relevant for small-scale and plot-level estimates used as training or accuracy assessment data for remote sensing applications that rely on emerging technologies such as airborne laser scanning. C1 [Berger, Ambros; Gschwantner, Thomas; Schadauer, Klemens] BFW Vienna, Fed Res & Training Ctr Forests Nat Hazards & Land, Vienna, Austria. [McRoberts, Ronald E.] USDA Forest Serv, No Res Stn, Washington, DC USA. RP Berger, A (reprint author), BFW Vienna, Fed Res & Training Ctr Forests Nat Hazards & Land, Vienna, Austria. EM ambros.berger@bfw.gv.at; thomas.gschwantner@bfw.gv.at; rmcroberts@fi.fed.us; klemens.schadauer@bfw.gv.at NR 45 TC 17 Z9 18 U1 1 U2 17 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2014 VL 60 IS 1 BP 14 EP 24 DI 10.5849/forsci.12-164 PG 11 WC Forestry SC Forestry GA 302LH UT WOS:000330605500003 ER PT J AU Breidenbach, J Anton-Fernandez, C Petersson, H McRoberts, RE Astrup, R AF Breidenbach, Johannes Anton-Fernandez, Clara Petersson, Hans McRoberts, Ronald E. Astrup, Rasmus TI Quantifying the Model-Related Variability of Biomass Stock and Change Estimates in the Norwegian National Forest Inventory SO FOREST SCIENCE LA English DT Article DE uncertainty; variance; mean squared error; biomass stock; biomass change; Monte Carlo simulation; parametric bootstrap ID NORWAY SPRUCE; UNCERTAINTY ESTIMATION; EXPANSION FACTORS; PICEA-ABIES; EQUATIONS; CARBON; FINLAND; ERROR AB National Forest Inventories (NFIs) provide estimates of forest parameters for national and regional scales. Many key variables of interest, such as biomass and timber volume, cannot be measured directly in the field. Instead, models are used to predict those variables from measurements of other field variables. Therefore, the uncertainty or variability of NFI estimates results not only from selecting a sample of the population but also from uncertainties in the models used to predict the variables of interest. The aim of this study was to quantify the model-related variability of Norway spruce (Picea abies [L.] Karst) biomass stock and change estimates for the Norwegian NFI. The model-related variability of the estimates stems from uncertainty in parameter estimates of biomass models as well as residual variability and was quantified using a Monte Carlo simulation technique. Uncertainties in model parameter estimates, which are often not available for published biomass models, had considerable influence on the model-related variability of biomass stock and change estimates. The assumption that the residual variability is larger than documented for the models and the correlation of within-plot model residuals influenced the model-related variability of biomass stock change estimates much more than estimates of the biomass stock. The larger influence on the stock change resulted from the large influence of harvests on the stock change, although harvests were observed rarely on the NFI sample plots in the 5-year period that was considered. In addition, the temporal correlation between model residuals due to changes in the allometry had considerable influence on the model-related variability of the biomass stock change estimate. The allometry may, however, be assumed to be rather stable over a 5-year period. Because the effects of model-related variability of the biomass stock and change estimates were much smaller than those of the sampling-related variability, efforts to increase the precision of estimates should focus on reducing the sampling variability. If the model-related variability is to be decreased, the focus should be on the tree fractions of living branches as well as stump and roots. C1 [Breidenbach, Johannes] Norwegian Forest & Landscape Inst, Climate Ctr, As, Norway. [Anton-Fernandez, Clara; Astrup, Rasmus] Norwegian Forest & Landscape Inst, As, Norway. [Petersson, Hans] Swedish Univ Agr Sci, S-90183 Umea, Sweden. [Astrup, Rasmus] USDA Forest Serv, No Res Stn, Washington, DC USA. RP Breidenbach, J (reprint author), Norwegian Forest & Landscape Inst, Climate Ctr, As, Norway. EM job@skogoglandskap.no; caf@skogoglandskap.no; hans.petersson@slu.se; rmcroberts@fifed.us; raa@skogoglandskap.no NR 34 TC 23 Z9 23 U1 4 U2 15 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2014 VL 60 IS 1 BP 25 EP 33 DI 10.5849/forsci.12-137 PG 9 WC Forestry SC Forestry GA 302LH UT WOS:000330605500004 ER PT J AU McRoberts, RE Westfall, JA AF McRoberts, Ronald E. Westfall, James A. TI Effects of Uncertainty in Model Predictions of Individual Tree Volume on Large Area Volume Estimates SO FOREST SCIENCE LA English DT Article DE allometric model; model parameter uncertainty; residual uncertainty; Monte Carlo simulation ID GROWING STOCK VOLUME; ALLOMETRIC EQUATIONS; FOREST INVENTORY; CARBON STOCKS; ABOVEGROUND BIOMASS; MEASUREMENT ERRORS; TROPICAL FORESTS; UNITED-STATES; GROWTH; PROJECTIONS AB Forest inventory estimates of tree volume for large areas are typically calculated by adding model predictions of volumes for individual trees. However, the uncertainty in the model predictions is generally ignored with the result that the precision of the large area volume estimates is overestimated. The primary study objective was to estimate the effects of model residual variability and model parameter uncertainty on large area volume estimates and their uncertainties for a study area in northeastern Minnesota, USA. Monte Carlo simulation approaches were used because of the complexities associated with multiple sources of uncertainty and the nonlinear nature of the models. Two conclusions were important. First, for this study, the effects of uncertainty in model predictions on the large area volume estimates and their uncertainties were small when the models were calibrated using an average of 100 or more observations per species and when the average proportion of variance explained by the models was at least 0.95. Second, large area estimates and their uncertainties based on coniferous/deciduous and nonspecific models deviated very little from large area estimates based on species-specific models. C1 [McRoberts, Ronald E.] USDA Forest Serv, No Res Stn, St Paul, MN 55101 USA. [Westfall, James A.] USDA Forest Serv, St Paul, MN USA. RP McRoberts, RE (reprint author), USDA Forest Serv, No Res Stn, St Paul, MN 55101 USA. EM rmcroberts@fs.fed.us; jameswesffall@fs.fed.us NR 45 TC 39 Z9 42 U1 2 U2 13 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2014 VL 60 IS 1 BP 34 EP 42 DI 10.5849/forsci.12-141 PG 9 WC Forestry SC Forestry GA 302LH UT WOS:000330605500005 ER PT J AU Gonzalez-Benecke, CA Gezan, SA Martin, TA Cropper, WP Samuelson, LJ Leduc, DJ AF Gonzalez-Benecke, Carlos A. Gezan, Salvador A. Martin, Timothy A. Cropper, Wendell P., Jr. Samuelson, Lisa J. Leduc, Daniel J. TI Individual Tree Diameter, Height, and Volume Functions for Longleaf Pine SO FOREST SCIENCE LA English DT Article DE Pinus palustris; individual-tree functions; general models; stand variables; merchantable stem volume ratio ID PLANTATIONS; EQUATIONS; MODEL; INTERVALS; ALBERTA AB Currently, little information is available to estimate individual tree attributes for longleaf pine (Pinus palustris Mill.), an important tree species of the southeastern United States. The majority of available models are local, relying on stem diameter outside bark at breast height (dbh, cm) and not including stand-level parameters. We developed a set of individual tree equations to predict tree height (H, m), stem diameter inside bark at 1.37 m height (dbh(IB), cm), stem volume outside bark (V-OB, m(3)), and stem volume inside bark (V-IB, m(3)), as well as functions to determine merchantable stem volume ratio (both outside and inside bark) from the stump to any top diameter. Local and general models are presented for each tree attribute. General models included stand-level parameters such as age, site index, dominant height, basal area, and tree density. The user should decide which model type to use, depending on data availability and level of accuracy desired. To our knowledge, this is the first comprehensive individual tree-level set of equations reported for longleaf pine trees, including local and general models, which can be applied to longleaf pine trees over a large geographical area and across a wide range of ages and stand characteristics. The system presented here provides important new tools for supporting future longleaf pine management decisions. C1 [Gonzalez-Benecke, Carlos A.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Gezan, Salvador A.; Martin, Timothy A.; Cropper, Wendell P., Jr.] Univ Florida, Gainesville, FL USA. [Samuelson, Lisa J.] Auburn Univ, Auburn, AL 36849 USA. [Leduc, Daniel J.] USDA Forest Serv, Washington, DC USA. RP Gonzalez-Benecke, CA (reprint author), Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. EM cgonzabe@ufl.edu; sgezan@ufl.edu; tamartin@ufl.edu; wcropper@ufl.edu; samuelj@auburn.edu; dleduc@fs.fed.us RI Cropper, Jr., Wendell/E-5952-2010; OI Cropper, Jr., Wendell/0000-0001-7851-7382; Martin, Timothy/0000-0002-7872-4194 FU US Department of Defense, through the Strategic Environmental Research and Development Program FX This research was supported by the US Department of Defense, through the Strategic Environmental Research and Development Program. The authors acknowledge the USDA Forest Service, Southern Research Station for their assistance and for providing the long-term data sets. Special thanks go to Mr. Tom Stokes, Ms. Ann Huyler, Mr. Justin Rathal, and Mr. Jake Blackstock for their help with field data collection. NR 36 TC 5 Z9 6 U1 1 U2 26 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2014 VL 60 IS 1 BP 43 EP 56 DI 10.5849/forsci.12-074 PG 14 WC Forestry SC Forestry GA 302LH UT WOS:000330605500006 ER PT J AU Wei, Y Bevers, M Nguyen, D Belval, E AF Wei, Yu Bevers, Michael Dung Nguyen Belval, Erin TI A Spatial Stochastic Programming Model for Timber and Core Area Management Under Risk of Fires SO FOREST SCIENCE LA English DT Article DE sample average approximation; mixed integer programming; harvest scheduling; spatial optimization ID OPTIMAL HARVEST; FOREST PATCHES; OPTIMIZATION; UNCERTAINTY; YIELD; STAND; RESTRICTIONS; COEFFICIENTS; ROTATION; LEVEL AB Previous stochastic models in harvest scheduling seldom address explicit spatial management concerns under the influence of natural disturbances. We employ multistage stochastic programming models to explore the challenges and advantages of building spatial optimization models that account for the influences of random stand-replacing fires. Our exploratory test models simultaneously consider timber harvest and mature forest core area objectives. Random fire samples are built into the model, creating a sample average approximation (SAA) formulation of our stochastic programming problem. Each model run reports first-period harvesting decisions along with recourse decisions for subsequent time periods reflecting the influence of stochastic fires. In each test, we solve 30 independent, identically distributed (i.i.d.) replicate models and calculate the persistence of period one solutions. Harvest decisions with the highest persistence are selected as the solution for each stand in a given test case. We explore various sample sizes in our SAA models. Monte Carlo simulations of these solutions are then run by fixing first-period solutions and solving new i.i.d. replicates. Multiple comparison tests identify the best first-period solution. Results indicate that integrating the occurrence of stand-replacing fire into forest harvest scheduling models can improve the quality of long-term spatially explicit forest plans. C1 [Wei, Yu; Dung Nguyen; Belval, Erin] Colorado State Univ, Ft Collins, CO 80523 USA. [Bevers, Michael] USDA Forest Serv, Rocky Mt Res Stn, Washington, DC USA. RP Wei, Y (reprint author), Colorado State Univ, Ft Collins, CO 80523 USA. EM yu.wei@colostate.edu; mbevers@fs.fed.us FU USDA Forest Service, Rocky Mountain Research Station [07-JV-11221611-259]; Colorado State University [07-JV-11221611-259]; McIntire-Stennis funding from Colorado Agriculture Experiment Station FX This project was supported by the Joint Venture Agreement 07-JV-11221611-259 between USDA Forest Service, Rocky Mountain Research Station, and Colorado State University, and also supported by the McIntire-Stennis funding from Colorado Agriculture Experiment Station. The authors are very grateful for the careful and helpful reviews from the anonymous associated editor and reviewer. NR 49 TC 2 Z9 2 U1 3 U2 26 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2014 VL 60 IS 1 BP 85 EP 96 DI 10.5849/forsci.12-124 PG 12 WC Forestry SC Forestry GA 302LH UT WOS:000330605500010 ER PT J AU Johnson, DW Walker, RF Glass, DW Stein, CM Murphy, JB Blank, RR Miller, WW AF Johnson, Dale W. Walker, Roger F. Glass, Dallas W. Stein, Chad M. Murphy, James B. Blank, Robert R. Miller, Watkins W. TI Effects of Thinning, Residue Mastication, and Prescribed Fire on Soil and Nutrient Budgets in a Sierra Nevada Mixed-Conifer Forest SO FOREST SCIENCE LA English DT Article DE nutrient exports; soil nutrients; carbon; nitrogen; phosphorus; potassium; calcium; magnesium; sulfur ID LAKE TAHOE BASIN; PRESCRIPTION FIRE; PINE FOREST; NITROGEN; WILDFIRE; CARBON; HARVEST; LOSSES AB The effects of thinning followed by residue mastication (THIN), prescribed fire (BURN), and thinning plus residue mastication plus burning (T+B) on nutrient budgets and resin-based (plant root simulator [PRS] probe) measurements of soil nutrient availability in a mixed-conifer forest were measured. Because of site differences, removals of carbon (C) and nutrients by harvesting were greater in the T+B than in the THIN (Mg) treatment. Harvesting caused greater exports of C, phosphorus, potassium (K), calcium (Ca), and magnesium than burning, harvesting and burning caused approximately equal exports of nitrogen (N), and burning caused greater exports of sulfur than harvesting. Burning caused greater volatilization losses of C and N from the T+B than from the BURN treatment because the addition of chips from mastication in the T+B treatment caused greater combustion of the native forest floor. The most significant effect of the chips on soil nutrients was the addition of K to the forest floor pools, which was also reflected in resin-based measurements of soil nutrients. Burning caused increases in sail NO3- -N, mineral N, Ca2+, and SO42- as measured by PRS probes, which persisted for 2 years after the burn. The more intense burning in the T+B treatment also caused elevated orthophosphate levels 2 years after the burn. Burning effects on K+ and Mg2+ were not significant. Overall, the most ecologically significant effect of treatment was the export of N from the combination of harvesting and burning in the T+B treatment, which totaled 932 kg ha(-1) and 13% of the total ecosystem N capital. C1 [Johnson, Dale W.] Univ Nevada, Dept Environm & Resource Sci, Reno, NV 89557 USA. [Walker, Roger F.; Glass, Dallas W.; Stein, Chad M.; Murphy, James B.; Miller, Watkins W.] Univ Nevada, Reno, NV 89557 USA. [Blank, Robert R.] USDA ARS, Reno, NV USA. RP Johnson, DW (reprint author), Univ Nevada, Dept Environm & Resource Sci, Reno, NV 89557 USA. EM dwj@unr.edu; rwalker@cabnr.unr.edu FU USDA Forest Service; Nevada Agricultural Experiment Station, University of Nevada, Reno, Nevada FX This research was supported by the USDA Forest Service and the Nevada Agricultural Experiment Station, University of Nevada, Reno, Nevada. We greatly appreciate the technical assistance of Dawn Hanseder, Matt Church, and Georgianna Stein. NR 34 TC 3 Z9 3 U1 4 U2 38 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2014 VL 60 IS 1 BP 170 EP 179 DI 10.5849/forsci.12-034 PG 10 WC Forestry SC Forestry GA 302LH UT WOS:000330605500018 ER PT J AU Sutton, WB Wang, Y Schweitzer, CJ Steen, DA AF Sutton, William B. Wang, Yong Schweitzer, Callie J. Steen, David A. TI Lizard Microhabitat and Microclimate Relationships in Southeastern Pine-Hardwood Forests Managed With Prescribed Burning and Thinning SO FOREST SCIENCE LA English DT Article DE reptile; William B. Bankhead National Forest; disturbance; forest management; Akaike's information criterion ID FUEL REDUCTION TREATMENTS; SHORT-TERM RESPONSE; SOUTHERN APPALACHIANS; EUMECES FASCIATUS; TIMBER HARVEST; 5-LINED SKINK; FIRE; REPTILES; DISTURBANCE; AMPHIBIANS AB Understanding the impacts of disturbances in forest ecosystems is essential for long-term biodiversity conservation. Many studies have evaluated wildlife responses to various disturbances but most generally do not use changes in microclimate features or microhabitat structure to explain these responses. We examined lizard responses to two common forest management practices (prescribed burning and thinning) in pine-hardwood forests of the Bankhead National Forest, Alabama, USA. Over 4 years, we captured 719 individual lizards representing seven species. Lizards exhibited species-specific responses to forest management: eastern fence lizard (Sceloporus undulatus) captures were positively associated with an increasing forest disturbance gradient and were greatest in thin with burn treatments, whereas little brown skink (Scincella lateral's) captures were positively associated with increasing liner depth and were greatest in control sites during the first year posttreatment. Green anole (Anolis carolinensis) captures increased in forest stands with concomitant increases in air temperature, whereas common five-lined skink (Plestiodon fasciatus) captures were related to coarse woody debris cover during posttreatment sampling. We were unable to detect a treatment response or microhabitat/microclimate associations for broad-headed skinks (Plestiodon laticeps). Through an information-theoretic approach, we were able to identify treatment effects along with changes in microclimate and microhabitat features potentially responsible for driving responses of southeastern lizard species to forest management. C1 [Sutton, William B.] Alabama A&M Univ, Dept Nat Resources & Environm Sci, Normal, AL 35762 USA. [Wang, Yong] Clemson Univ, Clemson, SC USA. [Schweitzer, Callie J.] USDA Forest Serv, Washington, DC USA. [Steen, David A.] Virginia Polytech Univ, Blacksburg, VA USA. RP Sutton, WB (reprint author), Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC 29631 USA. EM billsutton.wv@gmail.com; wang.aamu@gmail.com; cjschweitzer@fs.fed.us; davidasteen@gmail.com FU Environmental Protection Agency STAR Fellowship program; Southern Research Station of the USDA Forest Service; CREST program of the National Science Foundation [HRD-0420541] FX We thank M. Bolus, R. Hardman, and E. Larrivee for field assistance. We also thank R. Sisk, J. Rice, and S. Clark of the USDA Forest Service and the Alabama A&M University graduate students and staffwho provided assistance with the construction and installation oftrapping units, along with the employees of the Bankhead National Forest for providing logistical support. Funds for this study were provided by the Environmental Protection Agency STAR Fellowship program, the Southern Research Station of the USDA Forest Service, and the CREST program of the National Science Foundation (HRD-0420541). We thank three anonymous reviewers for comments on earlier versions of this manuscript. NR 57 TC 8 Z9 8 U1 11 U2 43 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2014 VL 60 IS 1 BP 180 EP 190 DI 10.5849/forsci.11-076 PG 11 WC Forestry SC Forestry GA 302LH UT WOS:000330605500019 ER PT J AU Riddick, EW Wu, ZX Rojas, MG AF Riddick, Eric W. Wu, Zhixin Rojas, M. Guadalupe TI Is Tetranychus urticae suitable prey for development and reproduction of naive Coleomegilla maculata? SO INSECT SCIENCE LA English DT Article DE behavior; biocontrol; Coccinellidae; nutrition; rearing ID ADALIA-BIPUNCTATA COLEOPTERA; ERIOPIS-CONNEXA COLEOPTERA; POTATO BEETLE COLEOPTERA; LADY BEETLE; CHLORELLA-VULGARIS; HARMONIA-AXYRIDIS; HOST-PLANT; MUSCA-DOMESTICA; SWEET CORN; COCCINELLIDAE AB The lady beetle Coleomegilla maculata De Geer is an omnivorous predator that could help suppress aphid and spider mite populations on plants in greenhouses, plantscapes or interiorscapes. We are assessing the nutritional requirements and feeding behavior of C. maculata on target prey (spider mites) and factitious (unnatural) food. Our ultimate goal is to develop an efficacious diet to mass produce C. maculata. In this study, we tested the hypothesis that Tetranychus urticae Koch (two-spotted spider mite) is not suitable prey for development and reproduction of naive C. maculata (i.e., with no prior exposure to T. urticae). Our objectives were to (i) provide baseline data on the effects of consuming T. urticae on C. maculata life history, (ii) to compare the effects of consuming all stages of T. urticae versus eggs of Musca domestica L. (common housefly), and (iii) to determine if the consumption of plant products was beneficial. We used C. maculata from a colony reared only on Ephestia kuehniella Zeller (Mediterranean flour moth) eggs. In experiments, C. maculata larvae were reared from the first instar to adult stage with prey/food in replicated arenas; adult females were paired with a single male with prey/food. The results showed that naive C. maculata readily attacked and consumed T. urticae. Nevertheless, T. urticae was less suitable than M. domestica eggs for C. maculata development and reproduction. Applying a synthetic pollen-Chlorella alga powder (SPCA) in arenas containing T. urticae appeared to boost C. maculata female development and reproduction. C1 [Riddick, Eric W.; Wu, Zhixin; Rojas, M. Guadalupe] ARS, Natl Biol Control Lab, USDA, Stoneville, MS 38776 USA. RP Riddick, EW (reprint author), ARS, Natl Biol Control Lab, USDA, 59 Lee Rd, Stoneville, MS 38776 USA. EM eric.riddick@ars.usda.gov OI riddick, eric/0000-0002-4795-961X NR 57 TC 4 Z9 6 U1 2 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9609 EI 1744-7917 J9 INSECT SCI JI Insect Sci. PD FEB PY 2014 VL 21 IS 1 BP 83 EP 92 DI 10.1111/1744-7917.12033 PG 10 WC Entomology SC Entomology GA 302TB UT WOS:000330626700010 PM 23955814 ER PT J AU Kim, S Kim, YS AF Kim, Sanghoon Kim, Yeon Seok TI Production of gliadin-poly(ethyl cyanoacrylate) nanoparticles for hydrophilic coating SO JOURNAL OF NANOPARTICLE RESEARCH LA English DT Article DE Nanoparticles; Cyanoacrylate; Hydrophilic coating; Wetting; Protein ID MOLECULAR-WEIGHT GLIADIN; GLUTENIN SUBUNITS; WHEAT; COMPOSITES; ETHANOL; PROTEIN; ZEIN AB Cyanoacrylate nanoparticles have been usually prepared by anionic polymerization initiated by hydroxyl ions derived from dissociation of water. In the current research, amine groups on the surface of gliadin aggregates were utilized as initiator for the polymerization of ethyl cyanoacrylate (ECA). Gliadin, a protein found in the endosperms of wheat (Triticum aestivum L.), is not soluble in water, but dissolves in aqueous ethanol in the form of aggregates. As a result of the reaction with ECA monomers, gliadin molecules are chemically bound to poly(ethyl cyanoacrylate) (PECA) chains. The nanoparticles thus produced are made up of block copolymers that are consisted of hydrophilic moiety (gliadin) and hydrophobic moiety (PECA). The suspension containing these nanoparticles showed an excellent coating capability on the surface of hydrophobic materials such as glass or plastics. A simple spray coating changed the wetting property of the material instantly and dramatically. Since both protein and poly(alkyl cyanoacrylate) are degradable polymers, the developed nanoparticles are degradable. C1 [Kim, Sanghoon] ARS, USDA, NCAUR, Peoria, IL 61604 USA. [Kim, Yeon Seok] NIST, Engn Lab, Gaithersburg, MD 20899 USA. RP Kim, S (reprint author), ARS, USDA, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM sanghoon.kim@ars.usda.gov; yeonseok.kim@nist.gov RI KIM, YEON SEOK/J-5864-2012 NR 23 TC 2 Z9 2 U1 2 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1388-0764 EI 1572-896X J9 J NANOPART RES JI J. Nanopart. Res. PD FEB 1 PY 2014 VL 16 IS 2 AR 2277 DI 10.1007/s11051-014-2277-6 PG 10 WC Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Chemistry; Science & Technology - Other Topics; Materials Science GA AA3EK UT WOS:000330975500001 ER PT J AU Wang, XZ Jiang, GL Green, M Scott, RA Hyten, DL Cregan, PB AF Wang, Xianzhi Jiang, Guo-Liang Green, Marci Scott, Roy A. Hyten, David L. Cregan, Perry B. TI Quantitative trait locus analysis of unsaturated fatty acids in a recombinant inbred population of soybean SO MOLECULAR BREEDING LA English DT Article DE Soybean; Oleic acid; Linoleic acid; Linolenic acid; QTL; Marker-assisted selection ID SUDDEN-DEATH SYNDROME; LOW LINOLENIC ACID; HIGH OLEIC-ACID; SEED OIL; FIELD-RESISTANCE; CYST-NEMATODE; QTL; GENES; IDENTIFICATION; GERMPLASM AB Soybean [Glycine max (L.) Merr.] is an important oilseed crop which produces about 30 % of the world's edible vegetable oil. The quality of soybean oil is determined by its fatty acid composition. Soybean oil high in oleic and low in linolenic fatty acids is desirable for human consumption and other uses. The objectives of this study were to identify quantitative trait loci (QTLs) for unsaturated fatty acids and to evaluate the genetic effects of single QTL and QTL combinations in soybean. A population of recombinant inbred lines derived from the cross of SD02-4-59 x A02-381100 was evaluated for fatty acid content in seven environments. In total, 516 polymorphic single nucleotide polymorphism markers, 477 polymorphic simple sequence repeat markers and three GmFAD3 genes were used to genotype the mapping population. By using the composite interval mapping and/or the interval mapping method, a total of 15 QTLs for the three unsaturated fatty acids were detected in more than two environments. Two QTLs for oleic acid on linkage groups G [chromosome (Chr) 18] (qOLE-G) and J (Chr 16) (qOLE-J), three QTLs for linoleic acid on linkage groups A1 (Chr 5) (qLLE-A1) and G (Chr 18) (qLLE-G-1 and qLLE-G-2), and five QTLs for linolenic acid on linkage groups C2 (Chr 6), D1a (Chr 1), D1b (Chr 2), F (Chr 13) and G (Chr 18) were consistently detected in at least three individual environments and the average data over all environments. Significant QTL x QTL interactions were not detected. However, significant QTL x environment interactions were detected for all the QTLs which were repeatedly detected. Some QTLs reported previously were confirmed, and seven new QTLs (two for oleic acid, two for linoleic acid and three for linolenic acid) were identified in this study. Comparisons of two-locus and three-locus combinations indicated that cumulative effects of QTLs were significant for all the three unsaturated fatty acids. QTL pyramiding by molecular marker-assisted breeding would be an appropriate strategy for the improvement of unsaturated fatty acids in soybean. C1 [Wang, Xianzhi; Jiang, Guo-Liang; Green, Marci] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Scott, Roy A.] USDA ARS, GWCC BLTSVL, Beltsville, MD 20705 USA. [Hyten, David L.; Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Jiang, GL (reprint author), S Dakota State Univ, Dept Plant Sci, Northern Plains Biostress Lab Bldg,Box 2140C, Brookings, SD 57007 USA. EM guo-liang.jiang@sdstate.edu OI Hyten, David/0000-0001-6324-9389 FU USB Seed Composition Research project via USDA/ARS; South Dakota Soybean Research and Promotion Council project FX This research was supported in part by USB Seed Composition Research project via USDA/ARS, and South Dakota Soybean Research and Promotion Council project. The determination of fatty acids was completed in the USDA/ARS Lab., in Peoria, IL and the Iowa State University DNA Facility in Ames, IA. Genotyping of three GmFAD3 genes was performed by Dr. Kristin Bilyeu's Lab., USDA/ARS in Columbia, MO. The parental line A02-381100 was provided by Dr. Walter Fehr, Iowa State University. Dr. Kelly Whiting, the United Soybean Board, reviewed the manuscript and provided constructive suggestions. NR 52 TC 10 Z9 13 U1 2 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD FEB PY 2014 VL 33 IS 2 BP 281 EP 296 DI 10.1007/s11032-013-9948-3 PG 16 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AA0NO UT WOS:000330792100003 ER PT J AU Thyssen, G McCarty, JC Li, P Jenkins, JN Fang, DD AF Thyssen, Gregory McCarty, Jack C. Li, Ping Jenkins, Johnie N. Fang, David D. TI Genetic mapping of non-target-site resistance to a sulfonylurea herbicide (Envoke (R)) in Upland cotton (Gossypium hirsutum L.) SO MOLECULAR BREEDING LA English DT Article DE Cotton; Herbicide resistance; Non-target-site resistance (NTSR); Acetolactate synthase (ALS); Acetohydroxyacid synthase (AHAS); Sulfonylurea; Envoke (R); Trifloxysulfuron sodium ID ACETOHYDROXYACID SYNTHASE; IMIDAZOLINONE RESISTANCE; ACETOLACTATE-SYNTHASE; CGA-362622; ARABIDOPSIS; METABOLISM; SELECTION; PLANTS; RICE; TRANSLOCATION AB Acetolactate synthase (ALS) is responsible for a rate-limiting step in the synthesis of essential branched-chain amino acids. Resistance to ALS-inhibiting herbicides, such as trifloxysulfuron sodium (Envoke(A (R))), can be due to mutations in the target gene itself. Alternatively, plants may exhibit herbicide tolerance through reduced uptake and translocation or increased metabolism of the herbicide. The diverse family of cytochrome P450 proteins has been suggested to be a source of novel herbicide metabolism in both weed and crop plants. In this study we generated a mapping population between resistant and susceptible cotton (Gossypium hirsutum L.) cultivars. We found that both cultivars possess identical and sensitive ALS sequences; however, the segregation of resistance in the F2 progeny was consistent with a single dominant gene. Here we report the closely linked genetic markers and approximate physical location on chromosome 20 of the source of Envoke herbicide susceptibility in the cotton cultivar Paymaster HS26. There are no P450 proteins in the corresponding region of the G. raimondii Ulbr. genome, suggesting that an uncharacterized molecular mechanism is responsible for Envoke herbicide tolerance in G. hirsutum. Identification of this genetic mechanism will provide new opportunities for exploiting sulfonylurea herbicides for management of both weeds and crop plants. C1 [Thyssen, Gregory; Li, Ping; Fang, David D.] USDA ARS SRRC, Cotton Fiber Biosci Res Unit, New Orleans, LA 70124 USA. [McCarty, Jack C.; Jenkins, Johnie N.] USDA ARS, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. RP Fang, DD (reprint author), USDA ARS SRRC, Cotton Fiber Biosci Res Unit, New Orleans, LA 70124 USA. EM david.fang@ars.usda.gov OI fang, david/0000-0003-0036-5459 FU United States Department of Agriculture-Agricultural Research Service CRIS project [6435-21000-017-00D] FX This research was funded by United States Department of Agriculture-Agricultural Research Service CRIS project 6435-21000-017-00D. We thank Mr. Russell Hayes for assisting with the field experiments. Our appreciation goes to Mrs. Sheron Simpson and Dr. Brian Scheffler at the Genomics and Bioinformatics Research Unit at Stoneville, MS for their excellent support in SSR marker analysis. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture, which is an equal opportunity provider and employer. NR 41 TC 2 Z9 2 U1 3 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD FEB PY 2014 VL 33 IS 2 BP 341 EP 348 DI 10.1007/s11032-013-9953-6 PG 8 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AA0NO UT WOS:000330792100007 ER PT J AU Uribe, P Jansky, S Halterman, D AF Uribe, Pedro Jansky, Shelley Halterman, Dennis TI Two CAPS markers predict Verticillium wilt resistance in wild Solanum species SO MOLECULAR BREEDING LA English DT Article DE Verticillium wilt; Potato early dying; Molecular marker; Disease resistance ID INTERSPECIFIC POTATO HYBRIDS; CULTIVATED POTATO; TOMATO VE1; DAHLIAE; MANAGEMENT; PETOTA; IDENTIFICATION; DISEASE; GENOME AB Verticillium wilt of potato is a persistent problem in the USA and worldwide. The disease, which is caused primarily by the fungus Verticillium dahliae, is difficult to manage, causes yield losses, and contaminates soil for subsequent plantings. Control strategies based on host resistance are seen as long-term, stable solutions, but difficult to achieve given the genetic nature of the host and the challenges associated with resistance evaluations. To provide breeders with marker-assisted selection opportunities, we generated a pair of cleaved amplified polymorphic sequence molecular markers within the coding region of Ve2, a potato gene with homology to the tomato Ve1 gene that confers resistance to V. dahliae. The position of the marker was determined according to the consensus sequences of Ve2 homologs of wild Solanum species with resistance to V. dahliae. Marker testing indicated their broad applicability, being able to track the resistance to V. dahliae in progeny containing genetic information derived from species S. chacoense, S. brevicaule, S. berthaultii, S. tarijense, and S. tuberosum. Furthermore, the two isolates of V. dahliae used in our inoculation experiments differed in virulence and demonstrated specificity for some wild potato species. Experimentation leading to the development of the markers and tests of their usefulness against a wide range of diploid potato germplasm is presented. C1 [Uribe, Pedro] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Jansky, Shelley] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Jansky, Shelley; Halterman, Dennis] ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. RP Halterman, D (reprint author), ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. EM dennis.halterman@ars.usda.gov FU ARS-State Cooperative Potato Research Grant FX Germplasm was provided by the NRSP-6 Potato Genebank. Funding was provided by an ARS-State Cooperative Potato Research Grant. NR 33 TC 7 Z9 7 U1 1 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD FEB PY 2014 VL 33 IS 2 BP 465 EP 476 DI 10.1007/s11032-013-9965-2 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AA0NO UT WOS:000330792100017 ER PT J AU Swiger, SL Hogsette, JA Butler, JF AF Swiger, S. L. Hogsette, J. A. Butler, J. F. TI Larval Distribution and Behavior of Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) Relative to Other Species on Florida Black Bear (Carnivora: Ursidae) Decomposing Carcasses SO NEOTROPICAL ENTOMOLOGY LA English DT Article DE Cochliomyia macellaria; decomposition; hairy maggot blow fly; larvae behavior; Lucilia coeruleiviridis ID COCHLIOMYIA-MACELLARIA DIPTERA; ARTHROPOD SUCCESSION; HAWAIIAN-ISLANDS; PIG CARRION; TEMPERATURE; OAHU; PATTERNS; USA AB Larval interactions of dipteran species, blow flies in particular, were observed and documented daily over time and location on five black bear carcasses in Gainesville, FL, USA, from June 2002 - September 2004. Cochliomyia macellaria (Fabricius) or Chrysomya megacephala (Fabricius) larvae were collected first, after which Chrysomya rufifacies (Macquart) oviposited on the carcasses in multiple locations (i.e., neck, anus, and exposed flesh) not inhabited already by the other blow fly larvae. Within the first week of decomposition, C. rufifacies larvae grew to a parts per thousand yen12 mm, filling the carcasses with thousands of larvae and replacing the other calliphorid larvae either through successful food source competition or by predation. As a result, C. macellaria and C. megacephala were not collected past their third instar feeding stage. The blow fly species, C. megacephala, C. macellaria, Lucilia caeruleiviridis (Macquart), Phormia regina (Meigen), Lucilia sericata (Meigen), and C. rufifacies, completed two developmental cycles in the 88.5-kg carcass. This phenomenon might serve to complicate or prevent the calculation of an accurate postmortem interval. C1 [Swiger, S. L.; Butler, J. F.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Hogsette, J. A.] USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Swiger, S. L.] Texas A&M AgriLife Extens, Stephenville, TX 76401 USA. RP Swiger, SL (reprint author), Texas A&M AgriLife Extens, 1229 North US Hwy 281, Stephenville, TX 76401 USA. EM slswiger@ag.tamu.edu NR 36 TC 4 Z9 4 U1 2 U2 16 PU ENTOMOLOGICAL SOC BRASIL PI LONDRINA, PA CAIXA POSTAL 481, 86001-970 LONDRINA,, PR, BRAZIL SN 1519-566X EI 1678-8052 J9 NEOTROP ENTOMOL JI Neotrop. Entomol. PD FEB PY 2014 VL 43 IS 1 BP 21 EP 26 DI 10.1007/s13744-013-0174-9 PG 6 WC Entomology SC Entomology GA 304GP UT WOS:000330735400003 PM 27193400 ER PT J AU Dror, DK Allen, LH AF Dror, Daphna K. Allen, Lindsay H. TI Dairy product intake in children and adolescents in developed countries: trends, nutritional contribution, and a review of association with health outcomes SO NUTRITION REVIEWS LA English DT Review DE adolescents; children; dairy; milk ID SCHOOL-AGED CHILDREN; PLASMA VITAMIN-B-12 CONCENTRATIONS; DIFFERENT MEAL PATTERNS; SOFT DRINK CONSUMPTION; BONE-MINERAL DENSITY; CALCIUM INTAKE; BEVERAGE CONSUMPTION; MILK CONSUMPTION; DIETARY CALCIUM; BLOOD-PRESSURE AB Despite its contribution to nutrient intake and status, consumption of milk and dairy products by children and adolescents in many countries has waned in recent decades, with a substantial proportion of youth failing to meet intake recommendations. Dairy products remain an important dietary source of multiple micronutrients, including calcium, phosphorus, magnesium, zinc, iodine, potassium, vitamin A, vitamin D, vitamin B-12, and riboflavin (vitamin B-2). In addition, dairy products provide children with energy, high-quality protein, and essential and nonessential fatty acids. A review of evidence was conducted to evaluate associations between milk or dairy product intake and health outcomes in children and adolescents. Results suggest a neutral or inverse association between consumption of milk and dairy products in children and adolescents and indicators of adiposity, incidence of dental caries, and hypertension. Available data indicate that dairy products are important for linear growth and bone health during childhood. Additional research - in particular, controlled intervention trials and long-term prospective cohort studies - is warranted to better understand how dairy intake affects health outcomes in children and adolescents. (C) 2013 International Life Sciences Institute C1 [Dror, Daphna K.; Allen, Lindsay H.] ARS, Allen Lab, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Dror, DK (reprint author), ARS, Allen Lab, USDA, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. EM dkdror@ucdavis.edu FU International Dairy Federation FX Funding for this review was provided by the International Dairy Federation. The authors have acted in best confidence to provide an unbiased and scientific evidence-based review, and the conclusions represent those of the authors alone. NR 173 TC 22 Z9 24 U1 7 U2 53 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD FEB PY 2014 VL 72 IS 2 BP 68 EP 81 DI 10.1111/nure.12078 PG 14 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 304NV UT WOS:000330754500002 PM 24330063 ER PT J AU Zanetti, M Harris, SS Dawson-Hughes, B AF Zanetti, Mayra Harris, Susan S. Dawson-Hughes, Bess TI Ability of vitamin D to reduce inflammation in adults without acute illness SO NUTRITION REVIEWS LA English DT Review DE 25-hydroxyvitamin D; C-reactive protein; inflammation; vitamin D ID C-REACTIVE PROTEIN; CARDIOVASCULAR-DISEASE; 25-HYDROXYVITAMIN D; OBESE SUBJECTS; OVERWEIGHT; HEALTH; SUPPLEMENTATION; CHOLECALCIFEROL AB In vitro studies and some clinical studies suggest that vitamin D plays an important role in reducing inflammation. The objective of this review was to examine recent evidence that vitamin D status influences the level of inflammation in adults without acute illness or injury. Five large cross-sectional studies and two randomized controlled trials are the focus of this review. Associations between 25-hydroxyvitamin D (25OHD) and inflammation markers are significant and inverse in study populations with low 25OHD levels (<21ng/mL). They are also inverse in adults with relatively high inflammation levels. These associations in the few available randomized controlled vitamin D intervention trials have been null; this may be because they were not examined in populations with sufficiently low levels of 25OHD or high levels of inflammation. (C) 2013 International Life Sciences Institute C1 [Zanetti, Mayra] Univ Fed Sao Paulo, Sao Paulo, Brazil. [Harris, Susan S.; Dawson-Hughes, Bess] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Dawson-Hughes, B (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM bess.dawson-hughes@tufts.edu FU U.S. Department of Agriculture [58-1950-0-014] FX This material is based upon work supported by the U.S. Department of Agriculture, under agreement No. 58-1950-0-014. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 20 TC 10 Z9 10 U1 2 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD FEB PY 2014 VL 72 IS 2 BP 95 EP 98 DI 10.1111/nure.12095 PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 304NV UT WOS:000330754500004 PM 24330160 ER PT J AU Dwyer, JT Woteki, C Bailey, R Britten, P Carriquiry, A Gaine, PC Miller, D Moshfegh, A Murphy, MM Edge, MS AF Dwyer, Johanna T. Woteki, Catherine Bailey, Regan Britten, Patricia Carriquiry, Alicia Gaine, P. Courtney Miller, Dennis Moshfegh, Alanna Murphy, Mary M. Edge, Marianne Smith TI Fortification: new findings and implications SO NUTRITION REVIEWS LA English DT Review DE daily values; dietary patterns; fortification; upper levels ID DIETARY-SUPPLEMENT USE; UNITED-STATES; FOOD FORTIFICATION; IRON-ABSORPTION; PEARL-MILLET; US ADULTS; BIOFORTIFICATION; NUTRIENTS; CHILDREN; FOLATE AB This article reviews the current landscape regarding food fortification in the United States; the content is based on a workshop sponsored by the North American Branch of the International Life Sciences Institute. Fortification of the food supply with vitamins and minerals is a public health strategy to enhance nutrient intakes of the population without increasing caloric intake. Many individuals in the United States would not achieve recommended micronutrient intakes without fortification of the food supply. The achievement and maintenance of a desirable level of nutritional quality in the nation's food supply is, thus, an important public health objective. While the addition of nutrients to foods can help maintain and improve the overall nutritional quality of diets, indiscriminate fortification of foods could result in overfortification or underfortification in the food supply and nutrient imbalances in the diets of individuals. Any changes in food fortification policy for micronutrients must be considered within the context of the impact they will have on all segments of the population and of food technology and safety applications and their limitations. This article discusses and evaluates the value of fortification, the success of current fortification efforts, and the future role of fortification in preventing or reversing nutrient inadequacies. (C) 2014 International Life Sciences Institute C1 [Dwyer, Johanna T.] Tufts Med Sch, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Med Ctr, Frances Stern Nutr Ctr, Boston, MA 02111 USA. [Woteki, Catherine] USDA, Dept Res Educ & Econ, Washington, DC 20250 USA. [Bailey, Regan] NIH, Bethesda, MD 20892 USA. [Britten, Patricia] USDA Ctr Nutr Policy & Promot, Alexandria, VA USA. [Carriquiry, Alicia] Iowa State Univ, Dept Stat, Ames, IA USA. [Gaine, P. Courtney] Int Life Sci Inst, Washington, DC USA. [Miller, Dennis] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. [Moshfegh, Alanna] USDA ARS, Beltsville Human Nutr Res, Beltsville, MD USA. [Murphy, Mary M.] Exponent Inc, Washington, DC USA. [Edge, Marianne Smith] Int Food Informat Council, Washington, DC USA. RP Dwyer, JT (reprint author), Tufts Med Sch, 800 Washington St,Box 783, Boston, MA 02111 USA. EM Jdwyer1@tuftsmedicalcenter.org OI Dwyer, Johanna/0000-0002-0783-1769 FU ILSI North America Committee on Fortification FX Support in preparing the manuscript was provided by the ILSI North America Committee on Fortification. NR 66 TC 10 Z9 10 U1 6 U2 61 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD FEB PY 2014 VL 72 IS 2 BP 127 EP 141 DI 10.1111/nure.12086 PG 15 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 304NV UT WOS:000330754500007 PM 24447229 ER PT J AU Cha, DH Adams, T Werle, CT Sampson, BJ Adamczyk, JJ Rogg, H Landolt, PJ AF Cha, Dong H. Adams, Todd Werle, Christopher T. Sampson, Blair J. Adamczyk, John J., Jr. Rogg, Helmuth Landolt, Peter J. TI A four-component synthetic attractant for Drosophila suzukii (Diptera: Drosophilidae) isolated from fermented bait headspace SO PEST MANAGEMENT SCIENCE LA English DT Article DE Drosophila suzukii; spotted wing drosophila; feeding attractant; trap; lure ID SPOTTED-WING DROSOPHILA; FIELD-EVALUATION; ACETIC-ACID; VINEGAR; IDENTIFICATION; VOLATILES; FRUITS; COMBINATIONS; ACETOIN; ETHANOL AB BACKGROUNDA mixture of wine and vinegar is more attractive than wine or vinegar to spotted wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), and ethanol and acetic acid are considered key to that attractiveness. In addition to ethanol and acetic acid, 13 other wine and vinegar volatiles are antennally active to D. suzukii and might be involved in food finding. RESULTSOut of the 13 antennally active chemicals, acetoin, ethyl lactate and methionol increased fly response to a mixture of acetic acid and ethanol in field trapping experiments. A five-component blend of acetic acid, ethanol, acetoin, ethyl lactate and methionol was as attractive as the starting mixture of wine and vinegar in field tests conducted in the states of Oregon and Mississippi. Subtracting ethyl lactate from the five-component blend did not reduce the captures of flies in the trap. However, subtracting any other compound from the blend significantly reduced the numbers of flies captured. CONCLUSIONThese results indicate that acetic acid, ethanol, acetoin and methionol are key olfactory cues for D. suzukii when attracted to wine and vinegar, which may be food-finding behavior leading flies to fermenting fruit in nature. It is anticipated that this four-component blend can be used as a highly attractive chemical lure for detection and management of D. suzukii. Published 2013. This article is a U.S. Government work and is in the public domain in the USA. C1 [Cha, Dong H.; Landolt, Peter J.] ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. [Adams, Todd; Rogg, Helmuth] Oregon Dept Agr, Salem, OR USA. [Werle, Christopher T.; Sampson, Blair J.; Adamczyk, John J., Jr.] ARS, Thad Cochran Southern Hort Res Lab, USDA, Poplarville, MS USA. RP Cha, DH (reprint author), ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. EM Dong.Cha@ars.usda.gov FU Washington Tree Fruit Research Commission FX The authors thank Jewel Brumley, Daryl Green, Heather Headrick and Donna Marshall for technical assistance, and Luis Monterde for use of his blueberry farm. Peter Teal and Charles Linn provided insightful suggestions for improvements to the manuscript. This research was supported in part by funding from the Washington Tree Fruit Research Commission. NR 31 TC 30 Z9 31 U1 6 U2 79 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD FEB PY 2014 VL 70 IS 2 BP 324 EP 331 DI 10.1002/ps.3568 PG 8 WC Agronomy; Entomology SC Agriculture; Entomology GA 304IL UT WOS:000330740200021 PM 23633121 ER PT J AU Strunk, JL Temesgen, H Andersen, HE Packalen, P AF Strunk, Jacob L. Temesgen, Hailemariam Andersen, Hans-Erik Packalen, Petteri TI Prediction of Forest Attributes with Field Plots, Landsat, and a Sample of Lidar Strips: A Case Study on the Kenai Peninsula, Alaska SO PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING LA English DT Article ID AIRBORNE LIGHT DETECTION; DISCRETE-RETURN LIDAR; SMALL-FOOTPRINT LIDAR; AERIAL PHOTOGRAPHS; INTERIOR ALASKA; MULTISPECTRAL DATA; BIOMASS ESTIMATION; HEDMARK COUNTY; TIMBER VOLUME; TANANA VALLEY AB In this study we demonstrate that sample strips of lidar in combination with Landsat can be used to predict forest attributes more precisely than from Landsat alone. While lidar and Landsat can each be used alone in vegetation mapping, the cost of wall to wall lidar may exceed users' financial resources, and Landsat may not support the desired level of prediction precision. We compare fitted linear models and k nearest neighbors (kNN) methods to link field measurements, lidar, and Landsat. We also compare 900 m(2) and 8,100 m(2) resolutions to link lidar to Landsat. An approach with lidar and Landsat together reduced estimates of residual variability for biomass by up to 36 percent relative to using Landsat alone. Linear models generally performed better than kNN approaches, and when linking lidar to Landsat, using 8,100 m(2) resolution performed better than 900 m(2). C1 [Strunk, Jacob L.; Temesgen, Hailemariam] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Andersen, Hans-Erik] USDA Forest Serv, PNW Res Stn, Seattle, WA USA. [Packalen, Petteri] Univ Eastern Finland, Fac Sci & Forestry, Joensuu, Finland. RP Strunk, JL (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. EM Jacob.Strunk@oregonstate.edu FU USFS-PNW research station FX Funding and data for this study were graciously provided by USFS-PNW research station. NR 40 TC 6 Z9 6 U1 3 U2 18 PU AMER SOC PHOTOGRAMMETRY PI BETHESDA PA 5410 GROSVENOR LANE SUITE 210, BETHESDA, MD 20814-2160 USA SN 0099-1112 J9 PHOTOGRAMM ENG REM S JI Photogramm. Eng. Remote Sens. PD FEB PY 2014 VL 80 IS 2 BP 143 EP 150 PG 8 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA AA2JB UT WOS:000330919800007 ER PT J AU Jun, TH Freewalt, K Michel, AP Mian, R AF Jun, Tae-Hwan Freewalt, Keith Michel, Andy P. Mian, Rouf TI Identification of novel QTL for leaf traits in soybean SO PLANT BREEDING LA English DT Article DE soybean; leaf area; leaf shape; specific leaf weight; single-nucleotide polymorphism ID RECOMBINANT INBRED LINES; CANOPY PHOTOSYNTHESIS; AGRONOMIC TRAITS; SEED YIELD; CULTIVARS; LINKAGE; WEIGHT; HERITABILITY; POPULATIONS; RESISTANCE AB Several leaf traits of soybean (Glycine max L. Merr.), including leaf area (LA), leaf shape (LS) and specific leaf weight (SLW) may be related to soybean yield. The objective of this study was to identify novel quantitative trait loci (QTL) for LA, LS and SLW in a recombinant inbred line (RIL) population. The phenotype data were collected in 2011 and 2012 for 93 F-7:10 RILs using a randomized complete block design with 2 replicates each year. Five hundred and sixteen single-nucleotide polymorphism (SNP) markers and the phenotype data were used to detect QTL using single marker analysis (SMA) and composite interval mapping (CIM). Single markers analysis identified 26 QTL for the three traits, of which 17 were novel and the rests were previously reported QTL. Most of these QTL were also identified by CIM. Most QTL reported in this study were in close proximity (<1cM) of one or more SNP markers. These publicly available SNP markers with close linkage to LA, LS and SLW should be useful for marker-assisted breeding for these traits. C1 [Jun, Tae-Hwan; Michel, Andy P.] Ohio State Univ, Dept Entomol, Wooster, OH 44691 USA. [Freewalt, Keith; Mian, Rouf] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [Mian, Rouf] USDA ARS, Wooster, OH 44691 USA. RP Mian, R (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, 1680 Madison Ave, Wooster, OH 44691 USA. EM rouf.mian@ars.usda.gov NR 29 TC 4 Z9 4 U1 2 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD FEB PY 2014 VL 133 IS 1 BP 61 EP 66 DI 10.1111/pbr.12107 PG 6 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA AA0QG UT WOS:000330800600008 ER PT J AU Zhang, JJ Yan, GH Wen, ZF An, YQ Singer, SD Liu, ZR AF Zhang, Jinjin Yan, Guohua Wen, Zhifeng An, Young-Qiang Singer, Stacy D. Liu, Zongrang TI Two tobacco AP1-like gene promoters drive highly specific, tightly regulated and unique expression patterns during floral transition, initiation and development SO PLANTA LA English DT Article DE AP1-like promoter; MADS-box genes; Transgenic plants; Inflorescence and floral organs; Flower-specific expression; Tissue-specific ablation ID MADS-BOX GENES; MULTIPLE PROTEIN FACTORS; FLOWER DEVELOPMENT; TRANSGENIC ARABIDOPSIS; 2ND INTRON; ELEMENTS; APETALA1; PLANTS; IDENTIFICATION; MERISTEMS AB The genetic engineering of agronomic traits requires an array of highly specific and tightly regulated promoters that drive expression in floral tissues. In this study, we isolated and characterized two tobacco APETALA1-like (AP1-like) promoters (termed NtAP1La and NtAP1Lb1) in transgenic plants using the GUS reporter system, along with tissue-specific ablation analyses. Our results demonstrated that the two promoters are active in floral inflorescences but not in vegetative apical meristems or other vegetative tissues, as reflected by strong GUS staining and DT-A-mediated ablation of apical shoot tips during reproductive but not vegetative growth. We also showed that the NtAP1Lb1 promoter was more active than NtAP1La in inflorescences, as the former yielded higher frequencies and greater phenotypic evidence of tissue ablation compared to the latter. We further revealed that both promoters were uniformly expressed in the meristems of stage 1 and 2 floral buds, but were differentially expressed in floral organs later during development. While NtAP1La was found to be active in stage 4-5 carpels, later becoming confined to ovary tissue from stage 9 onwards, NtAP1Lb1 activity was apparent in all floral organs from stages 3 to 7, becoming completely absent in all floral organs from stage 11 onward. Therefore, it seems that the two tobacco promoters have acquired similar but distinct inflorescence-, floral meristem- and floral organ-specific and development-dependent regulatory features without any leaky activity in vegetative tissues. These features are novel and have rarely been observed in other flower-specific promoters characterized to date. The potential application of these promoters for engineering sterility, increasing biomass production and modifying flower architecture, as well as their putative use in flower-specific transgene excision, will be discussed. C1 [Zhang, Jinjin; Yan, Guohua; Wen, Zhifeng; Liu, Zongrang] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Zhang, Jinjin] Shaanxi Normal Univ, Coll Life Sci, Xian 710062, Peoples R China. [Yan, Guohua] Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China. [Wen, Zhifeng] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China. [An, Young-Qiang] ARS, Plant Genet Res Unit, USDA, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Singer, Stacy D.] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada. RP Liu, ZR (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM zongrang.liu@ars.usda.gov FU USDA NIFA Bio-technology Risk Assessment Research grant [2009-01067] FX We thank Mr. Dennis Bennett for his excellent technical assistance. This study was funded by the USDA NIFA Bio-technology Risk Assessment Research grant (2009-01067). NR 49 TC 2 Z9 3 U1 0 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD FEB PY 2014 VL 239 IS 2 BP 469 EP 478 DI 10.1007/s00425-013-1995-9 PG 10 WC Plant Sciences SC Plant Sciences GA 303BP UT WOS:000330649600016 PM 24221021 ER PT J AU Tyagi, P Gore, MA Bowman, DT Campbell, BT Udall, JA Kuraparthy, V AF Tyagi, Priyanka Gore, Michael A. Bowman, Daryl T. Campbell, B. Todd Udall, Joshua A. Kuraparthy, Vasu TI Genetic diversity and population structure in the US Upland cotton (Gossypium hirsutum L.) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCUS; MULTILOCUS GENOTYPE DATA; NATURAL-POPULATIONS; MOLECULAR MARKERS; FIELD PERFORMANCE; CORE COLLECTION; JOINT LINKAGE; SSR MARKERS; CULTIVARS; GERMPLASM AB Key message Genetic diversity and population structure in the US Upland cotton was established and core sets of allelic richness were identified for developing association mapping populations in cotton. Elite plant breeding programs could likely benefit from the unexploited standing genetic variation of obsolete cultivars without the yield drag typically associated with wild accessions. A set of 381 accessions comprising 378 Upland (Gossypium hirsutum L.) and 3 G. barbadense L. accessions of the United States cotton belt were genotyped using 120 genome-wide SSR markers to establish the genetic diversity and population structure in tetraploid cotton. These accessions represent more than 100 years of Upland cotton breeding in the United States. Genetic diversity analysis identified a total of 546 alleles across 141 marker loci. Twenty-two percent of the alleles in Upland accessions were unique, specific to a single accession. Population structure analysis revealed extensive admixture and identified five subgroups corresponding to Southeastern, Midsouth, Southwest, and Western zones of cotton growing areas in the United States, with the three accessions of G. barbadense forming a separate cluster. Phylogenetic analysis supported the subgroups identified by STRUCTURE. Average genetic distance between G. hirsutum accessions was 0.195 indicating low levels of genetic diversity in Upland cotton germplasm pool. The results from both population structure and phylogenetic analysis were in agreement with pedigree information, although there were a few exceptions. Further, core sets of different sizes representing different levels of allelic richness in Upland cotton were identified. Establishment of genetic diversity, population structure, and identification of core sets from this study could be useful for genetic and genomic analysis and systematic utilization of the standing genetic variation in Upland cotton. C1 [Tyagi, Priyanka; Kuraparthy, Vasu] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Gore, Michael A.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Bowman, Daryl T.] North Carolina Fdn Seed Producers Inc, Zebulon, NC 27597 USA. [Campbell, B. Todd] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Udall, Joshua A.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. RP Kuraparthy, V (reprint author), N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. EM vasu_kuraparthy@ncsu.edu OI Bowman, Daryl T./0000-0001-6623-4035 FU Cotton Incorporated; NC Agricultural Research Service; NC Cotton Producers Association FX We thank Dr. Gina Brown-Guedira for providing access to the genotyping facility and Jared Smith, Kim Howell and Blake Bowen for their technical assistance. We are grateful to Cotton Incorporated, NC Agricultural Research Service and NC Cotton Producers Association for funding support. The authors would like to thank NC State University Plant Breeding Center and Monsanto Company for providing PhD assistantship to Priyanka Tyagi. NR 61 TC 25 Z9 28 U1 2 U2 39 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2014 VL 127 IS 2 BP 283 EP 295 DI 10.1007/s00122-013-2217-3 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 304CO UT WOS:000330723900003 PM 24170350 ER PT J AU Ruddle, P Whetten, R Cardinal, A Upchurch, RG Miranda, L AF Ruddle, Paul, II Whetten, Rebecca Cardinal, Andrea Upchurch, Robert G. Miranda, Lilian TI Effect of Delta 9-stearoyl-ACP-desaturase-C mutants in a high oleic background on soybean seed oil composition SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID STEARIC-ACID MUTANTS; GENETIC-LINKAGE MAP; HIGH-DENSITY; REGISTRATION; GENOME; INHERITANCE; TRAIT AB Key message Two new sources of elevated seed stearic acid were identified and the feasibility of an elevated stearic acid, high oleic acid germplasm was studied. Soybean [Glycine max (L.) Merr.] oil typically contains 2-4 % stearic acid. Oil with at least 20 % stearic acid is desirable because of its improved baking properties and health profile. This study identifies two new sources of high stearic acid and evaluates the interaction of high stearic and oleic acid alleles. TCHM08-1087 and TCHM08-755, high stearic acid 'Holladay' mutants, were crossed to FAM94-41-3, a line containing a point mutation in a seed-specific isoform of a Delta 9-stearoyl-acyl carrier protein-desaturase (SACPD-C). F-2-derived lines were evaluated for fatty acid content in four field environments. Sequencing of SACPDs in TCHM08-1087 and TCHM08-755 revealed distinct deletions of at least one megabase encompassing SACPD-C in both lines. After genotyping, the additive effect for stearic acid was estimated at +1.8 % for the SACPD-C point mutation and +4.1 % for the SACPD-C deletions. Average stearic acid in lines homozygous for the deletions was 12.2 %. A FAM94-41-3-derived line and TCHM08-1087-11, a selection from TCHM08-1087, were crossed to S09-2902-145, a line containing missense mutations in two fatty acid desaturases (FAD2-1A and FAD2-1B). F-1 plants were grown in a greenhouse and individual F-2 seed were genotyped and phenotyped. No interaction was observed between either FAD2-1A or FAD2-1B and any of the SACPD-C mutant alleles. Seed homozygous mutant for SACPD-C/FAD2-1A/FAD2-1B contained 12.7 % stearic acid and 65.5 % oleic acid while seed homozygous for the SACPD-C deletion and mutant for FAD2-1A and FAD2-1B averaged 10.4 % stearic acid and 75.9 % oleic acid. C1 [Ruddle, Paul, II; Cardinal, Andrea] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27965 USA. [Whetten, Rebecca; Upchurch, Robert G.; Miranda, Lilian] USDA ARS, Raleigh, NC 27607 USA. RP Miranda, L (reprint author), USDA ARS, 3127 Ligon St, Raleigh, NC 27607 USA. EM Lilian.Miranda@ars.usda.gov FU United Soybean Board; North Carolina Soybean Producers Association FX This research was supported in part by the United Soybean Board and the North Carolina Soybean Producers Association. The authors thank Dr. Joseph Burton for developing the FA-I and FA-J populations, Dr. Thomas Carter Jr. for supplying TCHM08-1087 and TCHM08-755 for use in this study and Dr. J. Grover Shannon for providing S09-2902-145. The authors thank W. Novitzky for assistance provided in the seed oil analysis and the part-time workers at the Soybean and Nitrogen Fixation Unit (USDA-ARS, N.C. State University, Raleigh) for assistance in collecting tissue for DNA isolation. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 41 TC 5 Z9 7 U1 0 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2014 VL 127 IS 2 BP 349 EP 358 DI 10.1007/s00122-013-2223-5 PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 304CO UT WOS:000330723900009 PM 24193357 ER PT J AU Lee, S Mian, MAR Sneller, CH Wang, H Dorrance, AE McHale, LK AF Lee, Sungwoo Mian, M. A. Rouf Sneller, Clay H. Wang, Hehe Dorrance, Anne E. McHale, Leah K. TI Joint linkage QTL analyses for partial resistance to Phytophthora sojae in soybean using six nested inbred populations with heterogeneous conditions SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; ASSOCIATION MAPPING POPULATION; GENOME-WIDE ASSOCIATION; SOUTHERN LEAF-BLIGHT; DISEASE RESISTANCE; GENETIC ARCHITECTURE; COMPLEX TRAITS; ROOT-ROT; LINE POPULATIONS; BROAD-SPECTRUM AB Partial resistance to Phytophthora sojae in soybean is controlled by multiple quantitative trait loci (QTL). With traditional QTL mapping approaches, power to detect such QTL, frequently of small effect, can be limited by population size. Joint linkage QTL analysis of nested recombinant inbred line (RIL) populations provides improved power to detect QTL through increased population size, recombination, and allelic diversity. However, uniform development and phenotyping of multiple RIL populations can prove difficult. In this study, the effectiveness of joint linkage QTL analysis was evaluated on combinations of two to six nested RIL populations differing in inbreeding generation, phenotypic assay method, and/or marker set used in genotyping. In comparison to linkage analysis in a single population, identification of QTL by joint linkage analysis was only minimally affected by different phenotypic methods used among populations once phenotypic data were standardized. In contrast, genotyping of populations with only partially overlapping sets of markers had a marked negative effect on QTL detection by joint linkage analysis. In total, 16 genetic regions with QTL for partial resistance against P. sojae were identified, including four novel QTL on chromosomes 4, 9, 12, and 16, as well as significant genotype-by-isolate interactions. Resistance alleles from PI 427106 or PI 427105B contributed to a major QTL on chromosome 18, explaining 10-45 % of the phenotypic variance. This case study provides guidance on the application of joint linkage QTL analysis of data collected from populations with heterogeneous assay conditions and a genetic framework for partial resistance to P. sojae. C1 [Lee, Sungwoo; Mian, M. A. Rouf; Sneller, Clay H.] Ohio State Univ, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [Mian, M. A. Rouf] ARS, Corn Soybean & Wheat Qual Res Unit, USDA, Wooster, OH 44691 USA. [Wang, Hehe; Dorrance, Anne E.] Ohio State Univ, Dept Plant Pathol, Wooster, OH 44691 USA. [McHale, Leah K.] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA. RP McHale, LK (reprint author), Ohio State Univ, Dept Hort & Crop Sci, 2021 Coffey Rd, Columbus, OH 43210 USA. EM lee.3546@osu.edu; rouf.mian@usda.ars.gov; sneller.5@osu.edu; hehe.wang@ars.usda.gov; dorrance.1@osu.edu; mchale.21@osu.edu RI McHale, Leah/D-9612-2012 FU United Soybean Board; Ohio Soybean Council FX This study was funded in part by United Soybean Board and Ohio Soybean Council through soybean producer's check-off dollars. We would like to acknowledge Dr. Perry Cregan's laboratory (USDA-ARS, Beltsville, MA) for the preliminary SNP genotyping of parental genotypes with 1,536 SNPs. We thank Dr. Steve St. Martin for advice on statistical analysis, and Dr. Huihui Li (Chinese Academy of Agricultural Science, Beijing, China) for helpful discussion of JICIM and provision of scripts for permutation analysis. We also thank T. Mendiola, L. Wallace, A. Bhupendra, A. Gunadi, A. Stasko, C. Phelan, C. Balk, C. Smith, D. Plewa, D. Veney, D. Wickramasinghe, and R. Schneider for technical assistance, and J. Whittier for conducting Illumina's GoldenGate (R) SNP genotyping at the MCIC/OARDC. NR 67 TC 10 Z9 13 U1 1 U2 31 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2014 VL 127 IS 2 BP 429 EP 444 DI 10.1007/s00122-013-2229-z PG 16 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 304CO UT WOS:000330723900015 PM 24247235 ER PT J AU Heslot, N Akdemir, D Sorrells, ME Jannink, JL AF Heslot, Nicolas Akdemir, Deniz Sorrells, Mark E. Jannink, Jean-Luc TI Integrating environmental covariates and crop modeling into the genomic selection framework to predict genotype by environment interactions SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID GENETIC VALUE; WHEAT; LOCI; SIMULATION; VARIETY; TRIALS; MAIZE AB Key message Development of models to predict genotype by environment interactions, in unobserved environments, using environmental covariates, a crop model and genomic selection. Application to a large winter wheat dataset. Genotype by environment interaction (G*E) is one of the key issues when analyzing phenotypes. The use of environment data to model G*E has long been a subject of interest but is limited by the same problems as those addressed by genomic selection methods: a large number of correlated predictors each explaining a small amount of the total variance. In addition, non-linear responses of genotypes to stresses are expected to further complicate the analysis. Using a crop model to derive stress covariates from daily weather data for predicted crop development stages, we propose an extension of the factorial regression model to genomic selection. This model is further extended to the marker level, enabling the modeling of quantitative trait loci (QTL) by environment interaction (Q*E), on a genome-wide scale. A newly developed ensemble method, soft rule fit, was used to improve this model and capture non-linear responses of QTL to stresses. The method is tested using a large winter wheat dataset, representative of the type of data available in a large-scale commercial breeding program. Accuracy in predicting genotype performance in unobserved environments for which weather data were available increased by 11.1 % on average and the variability in prediction accuracy decreased by 10.8 %. By leveraging agronomic knowledge and the large historical datasets generated by breeding programs, this new model provides insight into the genetic architecture of genotype by environment interactions and could predict genotype performance based on past and future weather scenarios. C1 [Heslot, Nicolas; Akdemir, Deniz; Sorrells, Mark E.; Jannink, Jean-Luc] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Heslot, Nicolas] Limagrain Europe, CS3911, F-63720 Chappes, France. [Jannink, Jean-Luc] Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Jannink, JL (reprint author), Cornell Univ, Dept Plant Breeding & Genet, 240 Emerson Hall, Ithaca, NY 14853 USA. EM nh269@cornell.edu; jeanluc.jannink@ars.usda.gov FU USDA-NIFA-AFRI [2009-65300-05661, 2011-68002-30029, 2005-05130]; Hatch project [149-449]; Limagrain Europe FX We thank Pierre Martre for providing the crop model. The reviewers provided excellent comments that significantly improved the paper. JRC-MARS-Meteorological Data Base-EC-JRC provided access to the interpolated meteorological data. This research was supported in part by USDA-NIFA-AFRI grants, award numbers 2009-65300-05661, 2011-68002-30029, and 2005-05130 and by Hatch project 149-449. Limagrain Europe provided financial support for N. Heslot. NR 80 TC 42 Z9 44 U1 1 U2 50 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2014 VL 127 IS 2 BP 463 EP 480 DI 10.1007/s00122-013-2231-5 PG 18 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 304CO UT WOS:000330723900017 PM 24264761 ER PT J AU Coble, AP Autio, A Cavaleri, MA Binkley, D Ryan, MG AF Coble, Adam P. Autio, Alisha Cavaleri, Molly A. Binkley, Dan Ryan, Michael G. TI Converging patterns of vertical variability in leaf morphology and nitrogen across seven Eucalyptus plantations in Brazil and Hawaii, USA SO TREES-STRUCTURE AND FUNCTION LA English DT Article DE Canopy position; Eucalyptus; Foliar morphology; Leaf mass per area; Leaf nitrogen; Vertical gradients ID HYDRAULIC LIMITATION HYPOTHESIS; TROPICAL RAIN-FOREST; TREE HEIGHT; PHOTOSYNTHETIC RATE; DECIDUOUS FOREST; GROWTH; AREA; FOLIAGE; WATER; AGE AB Across sites in Brazil and Hawaii, LMA and N (mass) were strongly correlated with height and shade index, respectively, which may help simplify canopy function modeling of Eucalyptus plantations. Within tree canopies, leaf mass per area (LMA) and leaf nitrogen per unit area (N (area)) commonly increase with height. Previous research has suggested that these patterns occur as a strategy to optimize carbon gain by allocating available resources to upper canopy leaves that are exposed to greater light availability. We tested three hypotheses about the influences of height, shade index (a proxy for light), and stand age on LMA and leaf nitrogen for even-aged Eucalyptus saligna and Eucalyptus grandis x urophylla plantations in Brazil and Hawaii, USA, spanning most of the environmental conditions found across 19.6 million ha of Eucalyptus spp. plantations around the world. Shade index was developed by incorporating canopy depth (inner-crown shading) and a tree height ratio relative to neighbor trees (shading from other trees). Across all sites and ages, leaf height accounted for 45 % of the variation in LMA, whereas shade index accounted for only 6 %. A combination of both factors was slightly better in accounting for LMA variation than height alone. LMA-height relationships among sites were strongest under greater light availability and in older stands. Leaf nitrogen per unit mass (N (mass)) consistently decreased with shade index, whereas N (area) showed no consistent pattern with height or shade index. These relationships indicate that N (mass) is primarily driven by light, while height is the primary driver for LMA. The general relationships between LMA and leaf N (mass) across all sites may simplify canopy function modeling of E. saligna and E. grandis x urophylla plantations. C1 [Coble, Adam P.; Autio, Alisha; Cavaleri, Molly A.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Binkley, Dan; Ryan, Michael G.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. [Binkley, Dan; Ryan, Michael G.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Ryan, Michael G.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Coble, AP (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, UJ Noblet Bldg,1400 Townsend Dr, Houghton, MI 49931 USA. EM apcoble@mtu.edu RI Ryan, Michael/A-9805-2008 OI Ryan, Michael/0000-0002-2500-6738 FU National Science Foundation [DEB93-06356, DEB97-0852]; CSIRO FX We thank the more than 100 people involved in Brazil Eucalyptus Potential Productivity Study (especially the project leader Jose Luiz Stape) and the companies that funded the work. Randy Senock, Christian Giardina, Holly Barnard, and James Fownes were particularly important in the Hawaii project, which was supported by National Science Foundation grants DEB93-06356 and DEB97-0852. MG Ryan was supported by a CSIRO McMaster's Fellowship during the preparation of this manuscript. NR 54 TC 6 Z9 8 U1 3 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0931-1890 EI 1432-2285 J9 TREES-STRUCT FUNCT JI Trees-Struct. Funct. PD FEB PY 2014 VL 28 IS 1 BP 1 EP 15 DI 10.1007/s00468-013-0925-6 PG 15 WC Forestry SC Forestry GA 302SC UT WOS:000330624200001 ER PT J AU Tiegs, SD Entrekin, SA Reeves, GH Kuntzsch, D Merritt, RW AF Tiegs, Scott D. Entrekin, Sally A. Reeves, Gordon H. Kuntzsch, Deyna Merritt, Richard W. TI Litter Decomposition, and Associated Invertebrate Communities, in Wetland Ponds of the Copper River Delta, Alaska (USA) (vol 33, pg 1151, 2013) SO WETLANDS LA English DT Correction C1 [Tiegs, Scott D.] Oakland Univ, Dept Biol Sci, Rochester, MI 48309 USA. [Entrekin, Sally A.] Univ Cent Arkansas, Dept Biol, Conway, AR 72035 USA. [Reeves, Gordon H.] US Forest Serv, USDA, Pacific Northwest Res Stn, Corvallis, OR 97331 USA. [Kuntzsch, Deyna] US Forest Serv, USDA, Cordova, AK 99574 USA. [Merritt, Richard W.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. RP Tiegs, SD (reprint author), Oakland Univ, Dept Biol Sci, Rochester, MI 48309 USA. EM tiegs@oakland.edu NR 1 TC 0 Z9 0 U1 2 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 EI 1943-6246 J9 WETLANDS JI Wetlands PD FEB PY 2014 VL 34 IS 1 BP 209 EP 209 DI 10.1007/s13157-013-0485-y PG 1 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 304IP UT WOS:000330740600019 ER PT J AU Ellis, MM Ivan, JS Schwartz, MK AF Ellis, Martha M. Ivan, Jacob S. Schwartz, Michael K. TI Spatially Explicit Power Analyses for Occupancy-Based Monitoring of Wolverine in the U.S. Rocky Mountains SO CONSERVATION BIOLOGY LA English DT Article DE detection probability; occupancy; population monitoring; population trends; sampling design ID PRESENCE-ABSENCE; BRITISH-COLUMBIA; GULO-GULO; TRENDS; CONSERVATION; POPULATIONS; DISTRIBUTIONS; CONSTRAINTS; CALIFORNIA; ABUNDANCE AB Conservation scientists and resource managers often have to design monitoring programs for species that are rare or patchily distributed across large landscapes. Such programs are frequently expensive and seldom can be conducted by one entity. It is essential that a prospective power analysis be undertaken to ensure stated monitoring goals are feasible. We developed a spatially based simulation program that accounts for natural history, habitat use, and sampling scheme to investigate the power of monitoring protocols to detect trends in population abundance over time with occupancy-based methods. We analyzed monitoring schemes with different sampling efforts for wolverine (Gulo gulo) populations in 2 areas of the U.S. Rocky Mountains. The relation between occupancy and abundance was nonlinear and depended on landscape, population size, and movement parameters. With current estimates for population size and detection probability in the northern U.S. Rockies, most sampling schemes were only able to detect large declines in abundance in the simulations (i.e., 50% decline over 10 years). For small populations reestablishing in the Southern Rockies, occupancy-based methods had enough power to detect population trends only when populations were increasing dramatically (e.g., doubling or tripling in 10 years), regardless of sampling effort. In general, increasing the number of cells sampled or the per-visit detection probability had a much greater effect on power than the number of visits conducted during a survey. Although our results are specific to wolverines, this approach could easily be adapted to other territorial species. C1 [Ellis, Martha M.] Univ Montana, Wildlife Biol Program, Missoula, MT 59801 USA. [Ivan, Jacob S.] Colorado Pk & Wildlife, Wildlife Res Ctr, Ft Collins, CO 80526 USA. [Schwartz, Michael K.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Ellis, MM (reprint author), Univ Montana, Wildlife Biol Program, Missoula, MT 59801 USA. EM martha.ellis@umontana.edu RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 FU RMRS; PECASE award FX We thank P. Lukacs, G. White, F. Allendorf, and L. Bailey for providing invaluable technical advice and J. Laake for implementing the "random occupancy dynamics" model into RMark so it could be used in this analysis. We thank R. Inman and J. Waller for generously sharing field data. We thank the RMRS and a PECASE award to M.K.S. for providing the initial funding for this effort. NR 47 TC 12 Z9 12 U1 8 U2 66 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0888-8892 EI 1523-1739 J9 CONSERV BIOL JI Conserv. Biol. PD FEB PY 2014 VL 28 IS 1 BP 52 EP 62 DI 10.1111/cobi.12139 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 297OX UT WOS:000330265900007 PM 24001256 ER PT J AU Wang, ZW Jiao, SY Han, GD Zhao, ML Ding, HJ Zhang, XJ Wang, XL Ayers, EL Willms, WD Havsatad, K Lata, A Liu, YZ AF Wang, Zhongwu Jiao, Shuying Han, Guodong Zhao, Mengli Ding, Haijun Zhang, Xinjie Wang, Xiaoliang Ayers, Eldon L. Willms, Walter D. Havsatad, Kris Lata, A. Liu, Yongzhi TI Effects of Stocking Rate on the Variability of Peak Standing Crop in a Desert Steppe of Eurasia Grassland SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Sheep grazing; Peak standing crop; Plant functional group; Stipa breviflora; Ecosystem stability ID PLANT-SPECIES DIVERSITY; FUNCTIONAL DIVERSITY; ECOSYSTEM STABILITY; VEGETATION RESPONSE; GRAZING MANAGEMENT; INNER-MONGOLIA; BIODIVERSITY; RAINFALL; QUALITY; SYSTEMS AB Proper grazing management practices can generate corresponding compensatory effects on plant community production, which may reduce inter-annual variability of productivity in some grassland ecosystems. However, it remains unclear how grazing influences plant community attributes and the variability of standing crop. We examined the effects of sheep grazing at four stocking rate treatments [control, 0 sheep ha(-1) month(-1); light (LG), 0.15 sheep ha(-1) month(-1); moderate (MG), 0.30 sheep ha(-1) month(-1); and heavy (HG), 0.45 sheep ha(-1) month(-1)] on standing crop at the community level and partitioned by species and functional groups, in the desert steppe of Inner Mongolia, China. The treatments were arranged in a completely randomized block design over a 9-year period. Standing crop was measured every August from 2004 to 2012. Peak standing crop decreased (P < 0.05) with increasing stocking rate; peak standing crop in the HG treatment decreased 40 % compared to the control. May-July precipitation explained at least 76 % of the variation in peak standing crop. MG and HG treatments resulted in a decrease (P < 0.05) in shrubs, semi-shrubs, and perennials forbs, and an increase (P < 0.05) in perennial bunchgrasses compared to the control. The coefficients of variation at plant functional group and species level in the LG and MG treatments were lower (P < 0.05) than in the control and HG treatments. Peak standing crop variability of the control and HG community were greatest, which suggested that LG and MG have greater ecosystem stability. C1 [Wang, Zhongwu; Han, Guodong; Zhao, Mengli; Ding, Haijun; Zhang, Xinjie; Wang, Xiaoliang] Inner Mongolia Agr Univ, Coll Ecol & Environm Sci, Dept Grassland Sci, Hohhot 010018, Inner Mongolia, Peoples R China. [Jiao, Shuying] Shandong Agr Univ, Coll Resources & Environm, Tai An 271018, Shandong, Peoples R China. [Ayers, Eldon L.] Tristar Land Technol, Dillon, MT USA. [Willms, Walter D.] Agr & Agri Food Canada, Lethrridge Res Ctr, Lethbridge, AB T1J 4B1, Canada. [Havsatad, Kris] ARS, USDA, Jornada Expt Range, Las Cruces, NM 88003 USA. [Lata, A.; Liu, Yongzhi] Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010010, Inner Mongolia, Peoples R China. RP Han, GD (reprint author), Inner Mongolia Agr Univ, Coll Ecol & Environm Sci, Dept Grassland Sci, Hohhot 010018, Inner Mongolia, Peoples R China. EM zhoungwuwang1979@163.com; grasslandkeylab@gmail.com FU National Nature Science Foundation of China [31260124, 31070413]; Specialized Research Fund for the Doctoral Program of Higher Education of China [20121515120015]; Inner Mongolia Nature Science Foundation [2011BS0409]; National Commonwealth Project [201003019, 200903060]; China Agricultural University [ZN201111]; Inner Mongolia Agricultural University [ZN201111]; National Technological Support Project [2012BAD13B02]; IMAU; National Basic Research Development Program (973 Program) [2007CB106800]; Inner Mongolia Science and Technological Committee, China FX This study was supported by the National Nature Science Foundation of China (31260124, 31070413), The Specialized Research Fund for the Doctoral Program of Higher Education of China (20121515120015), Inner Mongolia Nature Science Foundation (2011BS0409), The National Commonwealth Project (201003019, 200903060), Cooperative foundation between China Agricultural University and Inner Mongolia Agricultural University (ZN201111), The National Technological Support Project (2012BAD13B02), The Ph.D. start-up fund of IMAU, The National Basic Research Development Program (973 Program, No. 2007CB106800), and a grant from Inner Mongolia Science and Technological Committee, China. The authors would like to thank many students of Inner Mongolia Agricultural University for their efforts in collecting and analyzing data. The authors also want to thank all staff members of Siziwang Grassland Research Station for their help in our study. NR 34 TC 3 Z9 4 U1 4 U2 30 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD FEB PY 2014 VL 53 IS 2 BP 266 EP 273 DI 10.1007/s00267-013-0186-6 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA 298RV UT WOS:000330343000003 PM 24158269 ER PT J AU Adams, SB AF Adams, Susan B. TI Crayfish Use of Trash Versus Natural Cover in Incised, Sand-Bed Streams SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Crayfish; Habitat; Shelter; Garbage; Stream; Channel incision ID BODY-SIZE; PACIFASTACUS-LENIUSCULUS; SIGNAL CRAYFISH; PRIOR RESIDENCE; HABITAT USE; SHELTER; CHANNEL; FISH; COMPETITION; CAMBARIDAE AB Historic land use changes and subsequent river channelization created deeply incised, unstable stream channels largely devoid of natural cover throughout the Yazoo River basin, Mississippi, USA. Large trash (e.g., televisions, toilets, car parts) dumped in streams provided shelter for some aquatic fauna. To determine whether trash served as a surrogate for natural cover, I examined crayfish use of both cover types. I sampled crayfishes by kick-seining 2 x 1-m plots in three cover classes: trash, natural cover, and no cover. I captured 415 crayfishes from 136 of the 294 plots. Most crayfishes were in natural cover (253), followed by trash (154), and no-cover (8) plots. Trash use varied by crayfish genus and size. Frequencies of all size classes of Procambarus and of the smallest Cambarus were higher in natural cover than trash. Many of the smallest individuals were found in live root mats. As Cambarus and Orconectes grew, they shifted more toward trash, and the largest Orconectes size class was significantly more abundant than expected in trash. Trash served as "artificial reefs," providing cover for crayfishes and other fauna, but functioned differently than the remaining natural cover. The results confirmed that stream substrate did not provide adequate instream cover for crayfishes in the study area and suggested that high-quality natural cover for large crayfishes was in short supply, at least for some species. Land management that provides for abundant, ongoing input and retention of complex cover, such as trees and live roots, to stream channels should be beneficial for crayfish assemblages. C1 US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res, Oxford, MS 38655 USA. RP Adams, SB (reprint author), US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res, 1000 Front St, Oxford, MS 38655 USA. EM sadams01@fs.fed.us RI Adams, Susan/C-3559-2008 FU USDA Forest Service, Southern Research Station FX I thank the FS technicians, M. Bland, A. Commens-Carson, and G. McWhirter, and FS volunteer, J. Ryndock, who went beyond the call of duty in this sampling effort. The research was supported by the USDA Forest Service, Southern Research Station. NR 40 TC 0 Z9 0 U1 2 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD FEB PY 2014 VL 53 IS 2 BP 382 EP 392 DI 10.1007/s00267-013-0197-3 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA 298RV UT WOS:000330343000011 PM 24248331 ER PT J AU Yuan, L Huang, YB Loraamm, RW Nie, CW Wang, JH Zhang, JC AF Yuan, Lin Huang, Yanbo Loraamm, Rebecca W. Nie, Chenwei Wang, Jihua Zhang, Jingcheng TI Spectral analysis of winter wheat leaves for detection and differentiation of diseases and insects SO FIELD CROPS RESEARCH LA English DT Article DE Hyperspectral; Powdery mildew; Yellow rust; Aphid; Fisher linear discrimination analysis (FLDA); Partial least square regression (PLSR) ID QUERCUS-AGRIFOLIA LEAVES; SITU HYPERSPECTRAL DATA; TRITICUM-AESTIVUM L.; REFLECTANCE MEASUREMENTS; POWDERY MILDEW; YELLOW RUST; WATER STATUS; LEAF; IDENTIFICATION; STRESS AB Yellow rust (Puccinia striiformis f. sp. Tritici), powdery mildew (Blumeria graminis) and wheat aphid (Sitobion avenae F.) infestation are three serious conditions that have a severe impact on yield and grain quality of winter wheat worldwide. Discrimination among these three stressors is of practical importance, given that specific procedures (i.e. adoption of fungicide and insecticide) are needed to treat different diseases and insects. This study examines the potential of hyperspectral sensor systems in discriminating these three stressors at leaf level. Reflectance spectra of leaves infected with yellow rust, powdery mildew and aphids were measured at the early grain filling stage. Normalization was performed prior to spectral analysis on all three groups of samples for removing differences in the spectral baseline among different cultivars. To obtain appropriate bands and spectral features (SFs) for stressor discrimination and damage intensity estimation, a correlation analysis and an independent t-test were used jointly. Based on the most efficient bands/SFs, models for discriminating stressors and estimating stressor intensity were established by Fisher's linear discriminant analysis (FLDA) and partial least square regression (PLSR), respectively. The results showed that the performance of the discrimination model was satisfactory in general, with an overall accuracy of 0.75. However, the discrimination model produced varied classification accuracies among different types of diseases and insects. The regression model produced reasonable estimates of stress intensity, with an R-2 of 0.73 and a RMSE of 0.148. This study illustrates the potential use of hyperspectral information in discriminating yellow rust, powdery mildew and wheat aphid infestation in winter wheat. In practice, it is important to extend the discriminative analysis from leaf level to canopy level. (C) 2013 Elsevier B.V. All rights reserved. C1 [Yuan, Lin; Nie, Chenwei; Wang, Jihua; Zhang, Jingcheng] Beijing Acad Agr & Forestry Sci, Beijing Res Ctr Informat Technol Agr, Beijing 100097, Peoples R China. [Yuan, Lin; Nie, Chenwei; Wang, Jihua; Zhang, Jingcheng] Zhejiang Univ, Inst Remote Sensing & Informat Applicat, Hangzhou 310058, Zhejiang, Peoples R China. [Huang, Yanbo] ARS, USDA, CPSRU, Stoneville, MS 38776 USA. [Loraamm, Rebecca W.] Univ S Florida, Dept Geog Environm & Planning, Tampa, FL 33620 USA. RP Zhang, JC (reprint author), Beijing Acad Agr & Forestry Sci, Beijing Res Ctr Informat Technol Agr, Beijing 100097, Peoples R China. EM zhangjc@nercita.org.cn FU National Natural Science Foundation of China [41301476, 41271412]; Beijing Natural Science Foundation [4132029] FX This work was financially supported by the National Natural Science Foundation of China (Project no. 41301476 and 41271412), Beijing Natural Science Foundation (Project no. 4132029). The authors are grateful to Mr. Weiguo Li and Mrs. Hong Chang for data collection. NR 34 TC 12 Z9 18 U1 4 U2 48 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD FEB 1 PY 2014 VL 156 BP 199 EP 207 DI 10.1016/j.fcr.2013.11.012 PG 9 WC Agronomy SC Agriculture GA 297LI UT WOS:000330256500020 ER PT J AU Charles, T Grimm, CC McBride, J Landry, S Maleki, SJ AF Charles, Tysheena Grimm, Casey C. McBride, Jane Landry, Samuel Maleki, Soheila J. TI Simulated Roasting Affects Patient IgE Binding To Ara h 2 SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 28-MAR 04, 2014 CL San Diego, CA SP Amer Acad Allergy Asthma & Immunol C1 [Charles, Tysheena; Landry, Samuel] Tulane Univ, Hlth Sci Ctr, New Orleans, LA 70118 USA. [Charles, Tysheena; Grimm, Casey C.; McBride, Jane; Maleki, Soheila J.] ARS, USDA, SRRC, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2014 VL 133 IS 2 SU S MA 407 BP AB116 EP AB116 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 297FO UT WOS:000330241300404 ER PT J AU Chung, SY Mattison, CP Reed, S Wasserman, RL Desormeaux, WA AF Chung, Si-Yin Mattison, Christopher P. Reed, Shawndrika Wasserman, Richard L. Desormeaux, Wendy A. TI Effect Of Oleic Acid On The Allergenic Properties Of Peanut and Cashew Allergens SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 28-MAR 04, 2014 CL San Diego, CA SP Amer Acad Allergy Asthma & Immunol C1 [Chung, Si-Yin; Mattison, Christopher P.; Reed, Shawndrika; Desormeaux, Wendy A.] USDA ARS SRRC, New Orleans, LA USA. [Wasserman, Richard L.] Allergy Immunol Res Ctr North Texas, Dallas, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2014 VL 133 IS 2 SU S MA 387 BP AB111 EP AB111 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 297FO UT WOS:000330241300384 ER PT J AU Fleming, E Ruiter, B Hurlburt, BK Maleki, SJ Shreffler, WG AF Fleming, Elizabeth Ruiter, Bert Hurlburt, Barry K. Maleki, Soheila J. Shreffler, Wayne G. TI Monocytes From Peanut-Allergic Patients Express Higher Levels Of RALDH2 In Response To Peanut Protein Than Monocytes From Tolerant Subjects SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 28-MAR 04, 2014 CL San Diego, CA SP Amer Acad Allergy Asthma & Immunol C1 [Fleming, Elizabeth; Ruiter, Bert; Shreffler, Wayne G.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Hurlburt, Barry K.; Maleki, Soheila J.] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2014 VL 133 IS 2 SU S MA 321 BP AB92 EP AB92 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 297FO UT WOS:000330241300319 ER PT J AU Hurlburt, BK Cheng, HP Offermann, L Chruszcz, M Santos, AF Lack, G Maleki, SJ AF Hurlburt, Barry K. Cheng, Hsiaopo Offermann, Lesa Chruszcz, Maksymilian Santos, Alexandra F. Lack, Gideon Maleki, Soheila J. TI Epitope Mapping The Peanut Panallergen Ara h 8 SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 28-MAR 04, 2014 CL San Diego, CA SP Amer Acad Allergy Asthma & Immunol C1 [Hurlburt, Barry K.; Cheng, Hsiaopo; Maleki, Soheila J.] ARS, USDA, SRRC, New Orleans, LA USA. [Offermann, Lesa; Chruszcz, Maksymilian] Univ S Carolina, Columbia, SC 29208 USA. [Santos, Alexandra F.] Kings Coll London, London WC2R 2LS, England. [Lack, Gideon] St Thomas Hosp, London, England. NR 0 TC 0 Z9 0 U1 1 U2 3 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2014 VL 133 IS 2 SU S MA 406 BP AB116 EP AB116 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 297FO UT WOS:000330241300403 ER PT J AU Mattison, CP Desormeaux, WA Wasserman, RL Grimm, CC AF Mattison, Christopher P. Desormeaux, Wendy A. Wasserman, Richard L. Grimm, Casey C. TI Characterizing The Effect Of Sodium Sulfite On Cashew Allergens SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 28-MAR 04, 2014 CL San Diego, CA SP Amer Acad Allergy Asthma & Immunol C1 [Mattison, Christopher P.; Desormeaux, Wendy A.; Grimm, Casey C.] USDA ARS SRRC, New Orleans, LA USA. [Wasserman, Richard L.] Allergy Immunol Res Ctr North Texas, Dallas, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2014 VL 133 IS 2 SU S MA 403 BP AB115 EP AB115 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 297FO UT WOS:000330241300400 ER PT J AU Rose, KM Plaisance, C Grimm, CC Cheng, HO Charles, T Jortani, SA Maleki, SJ AF Rose, Kelli M. Plaisance, Christian Grimm, Casey C. Cheng, Hsiaopo Charles, Tysheena Jortani, Saeed A. Maleki, Soheila J. TI Detection Of Peanut Allergens In Breast Milk and Saliva SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 28-MAR 04, 2014 CL San Diego, CA SP Amer Acad Allergy Asthma & Immunol C1 [Rose, Kelli M.] Tulane Univ, New Orleans, LA 70118 USA. [Plaisance, Christian; Grimm, Casey C.; Cheng, Hsiaopo; Charles, Tysheena; Maleki, Soheila J.] USDA ARS SRRC, New Orleans, LA USA. [Jortani, Saeed A.] Univ Louisville, Louisville, KY 40292 USA. NR 0 TC 0 Z9 0 U1 1 U2 6 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2014 VL 133 IS 2 SU S MA 401 BP AB114 EP AB114 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 297FO UT WOS:000330241300398 ER PT J AU Ruiter, B Fleming, E Hurlburt, BK Maleki, SJ Shreffler, WG AF Ruiter, Bert Fleming, Elizabeth Hurlburt, Barry K. Maleki, Soheila J. Shreffler, Wayne G. TI Peanut Protein Induces Expression Of RALDH2 In Human Dendritic Cells In a TLR2-Dependent Manner SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 28-MAR 04, 2014 CL San Diego, CA SP Amer Acad Allergy Asthma & Immunol C1 [Ruiter, Bert; Fleming, Elizabeth; Shreffler, Wayne G.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Hurlburt, Barry K.; Maleki, Soheila J.] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2014 VL 133 IS 2 SU S MA 322 BP AB92 EP AB92 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 297FO UT WOS:000330241300320 ER PT J AU Lamont, EA Janagama, HK Ribeiro-Lima, J Vulchanova, L Seth, M Yang, M Kurmi, K Waters, WR Thacker, T Sreevatsan, S AF Lamont, Elise A. Janagama, Harish K. Ribeiro-Lima, Joao Vulchanova, Lucy Seth, Meetu Yang, My Kurmi, Kiran Waters, W. Ray Thacker, Tyler Sreevatsan, Srinand TI Circulating Mycobacterium bovis Peptides and Host Response Proteins as Biomarkers for Unambiguous Detection of Subclinical Infection SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID GAMMA-INTERFERON ASSAY; TUBERCULOSIS; CATTLE; DIAGNOSIS; BIOSYNTHESIS AB Bovine tuberculosis remains one of the most damaging diseases to agriculture, and there is also a concern for human spillover. A critical need exists for rapid, thorough, and inexpensive diagnostic methods capable of detecting and differentiating Mycobacterium bovis infection from other pathogenic and environmental mycobacteria at multiple surveillance levels. In a previous study, Seth et al. (PLoS One 4:e5478, 2009, doi:10.1371/journal.pone.0005478) identified 32 host peptides that specifically increased in the blood serum of M. bovis-infected animals). In the current study, 16 M. bovis proteins were discovered in the blood serum proteomics data sets. A large-scale validation analysis was undertaken for selected host and M. bovis proteins using a cattle serum repository containing M. bovis (n = 128), Mycobacterium kansasii (n = 10), and Mycobacterium avium subsp. paratuberculosis (n = 10), cases exposed to M. bovis (n = 424), and negative controls (n = 38). Of the host biomarkers, vitamin D binding protein (VDBP) showed the greatest sensitivity and specificity for M. bovis detection. Circulating M. bovis proteins, specifically polyketide synthetase 5, detected M. bovis-infected cattle with little to no seroreactivity against M. kansasii- and M. avium subsp. paratuberculosis-infected animals. These data indicate that host and pathogen serum proteins can serve as reliable biomarkers for tracking M. bovis infection in animal populations. C1 [Lamont, Elise A.; Ribeiro-Lima, Joao; Yang, My; Kurmi, Kiran; Sreevatsan, Srinand] Univ Minnesota, Dept Vet Populat Med, St Paul, MN 55108 USA. [Vulchanova, Lucy; Sreevatsan, Srinand] Univ Minnesota, Dept Vet Biomed Sci, St Paul, MN 55108 USA. [Janagama, Harish K.] Texas A&M Hlth Sci Ctr, Dept Microbial & Mol Pathogenesis, Bryan, TX USA. [Seth, Meetu] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA USA. [Waters, W. Ray; Thacker, Tyler] USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Sreevatsan, S (reprint author), Univ Minnesota, Dept Vet Populat Med, St Paul, MN 55108 USA. EM sreev001@umn.edu OI Thacker, Tyler/0000-0001-6779-7649; Sreevatsan, Srinand/0000-0002-5162-2403 FU Minnesota Rapid Agricultural Response Fund (Minnesota Agricultural Experiment Station); Morris Animal Foundation Wildlife Fund [D14ZO-086]; USDA [614056A] FX This study was funded by the Minnesota Rapid Agricultural Response Fund (Minnesota Agricultural Experiment Station), the Morris Animal Foundation Wildlife Fund (D14ZO-086), and the USDA (grant 614056A, awarded to S.S.). NR 29 TC 6 Z9 6 U1 1 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD FEB PY 2014 VL 52 IS 2 BP 536 EP 543 DI 10.1128/JCM.02433-13 PG 8 WC Microbiology SC Microbiology GA 300DI UT WOS:000330444200022 PM 24478485 ER PT J AU Castillo, JA Epps, CW Davis, AR Cushman, SA AF Castillo, Jessica A. Epps, Clinton W. Davis, Anne R. Cushman, Samuel A. TI Landscape effects on gene flow for a climate-sensitive montane species, the American pika SO MOLECULAR ECOLOGY LA English DT Article DE causal modelling; cdpop; landscape genetics; Mantel tests ID BIOCLIMATE ENVELOPE MODELS; OCHOTONA-PRINCEPS; GREAT-BASIN; POPULATION-GENETICS; COMPLEX LANDSCAPES; COMPUTER-PROGRAM; MANTEL TESTS; DISPERSAL; PATTERNS; RESISTANCE AB Climate change is arguably the greatest challenge to conservation of our time. Most vulnerability assessments rely on past and current species distributions to predict future persistence but ignore species' abilities to disperse through landscapes, which may be particularly important in fragmented habitats and crucial for long-term persistence in changing environments. Landscape genetic approaches explore the interactions between landscape features and gene flow and can clarify how organisms move among suitable habitats, but have suffered from methodological uncertainties. We used a landscape genetic approach to determine how landscape and climate-related features influence gene flow for American pikas (Ochotona princeps) in Crater Lake National Park. Pikas are heat intolerant and restricted to cool microclimates; thus, range contractions have been predicted as climate changes. We evaluated the correlation between landscape variables and genetic distance using partial Mantel tests in a causal modelling framework, and used spatially explicit simulations to evaluate methods of model optimization including a novel approach based on relative support and reciprocal causal modelling. We found that gene flow was primarily restricted by topographic relief, water and west-facing aspects, suggesting that physical restrictions related to small body size and mode of locomotion, as well as exposure to relatively high temperatures, limit pika dispersal in this alpine habitat. Our model optimization successfully identified landscape features influencing resistance in the simulated data for this landscape, but underestimated the magnitude of resistance. This is the first landscape genetic study to address the fundamental question of what limits dispersal and gene flow in the American pika. C1 [Castillo, Jessica A.; Epps, Clinton W.; Davis, Anne R.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. RP Castillo, JA (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, 104 Nash Hall, Corvallis, OR 97331 USA. EM jessica.castillo@oregonstate.edu FU NPS Climate Change Response Program (PMIS) [163377]; US Geological Survey [G11AC20397]; National Science Foundation GRFP FX We thank M. Jeffress, L. Garrett, C. Ray, M. Lonneker, T. Rodhouse, R. Anthony, C. Millar, C. Baker, C. Peterson, W. Woolen and National Parks Service (NPS) staff and field technicians who contributed to the 'Pikas in Peril' project. Also, we thank L. Waits and three anonymous reviewers. This project was funded by the NPS Climate Change Response Program (PMIS # 163377), the US Geological Survey Park-Oriented Biological Support (POBS) Program (Grant # G11AC20397) and the National Science Foundation GRFP (J. Castillo). NR 68 TC 41 Z9 42 U1 10 U2 118 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD FEB PY 2014 VL 23 IS 4 BP 843 EP 856 DI 10.1111/mec.12650 PG 14 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 297OE UT WOS:000330264000009 PM 24383818 ER PT J AU Mahoney, NE Gee, WS Higbee, BS Beck, JJ AF Mahoney, Noreen E. Gee, Wai S. Higbee, Bradley S. Beck, John J. TI Ex situ volatile survey of ground almond and pistachio hulls for emission of spiroketals: Analysis of hull fatty acid composition, water content, and water activity SO PHYTOCHEMISTRY LETTERS LA English DT Article DE Almond; Fungi; Hull; Pistachio; Spiroketal; Volatiles ID NAVEL ORANGEWORM; ASPERGILLUS; GROWTH; FUNGI; OIL AB The spiroketal conophthorin has recently been implicated as an important semiochemical of the navel orangeworm moth (Amyelois transitella), a major insect pest to California tree nuts. Additionally, new evidence demonstrates that fungal spores in the presence of linoleic acid produce conophthorin. Numerous investigations have analyzed the volatile emissions of almonds and pistachios under varying conditions, yet there are few reports of conophthorin as a volatile component. Previous studies by our laboratories have suggested almond hulls may be a source of conophthorin production. Accordingly, the volatile emissions of ex situ almond and pistachio ground hulls were surveyed at several developmental stages. Each ground sample was analyzed at various intervals to determine if conophthorin was produced. The almond and pistachio samples were presumed to have a natural fungal bouquet present. Additionally, the fatty acid composition, water content, and water activity of the hulls were analyzed for each sample. Conophthorin and the structurally similar compound chalcogran were detected from almond hulls and shells, but not from the pistachio samples. The almond and pistachio hulls were investigated for four fatty acid components - palmitic, oleic, linoleic, and linolenic. The fatty acid composition of almond hulls varied greatly throughout the growing season, whereas the composition of pistachio hulls remained relatively constant. Both water content and activity were constant in early stages of almond growth then dropped in the later stages of hull split. Spiroketal emission along with other associated volatiles is discussed. This is the first report of the fatty acid composition, water content, and water activity of developing almond and pistachio hulls. Published by Elsevier Ireland Ltd on behalf of Phytochemical Society of Europe. C1 [Mahoney, Noreen E.; Gee, Wai S.; Beck, John J.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Higbee, Bradley S.] Paramount Farming Co, Bakersfield, CA 93308 USA. RP Beck, JJ (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM john.beck@ars.usda.gov FU USDA-ARS CRIS Project [5325-42000-037-00D]; TFCA [5325-42000-037-05]; Almond Board of California [TFCA 5325-42000-037-07]; California Pistachio Research Board; portions of RCA [5325-42000-037-13]; California Department of Food and Agriculture FX The authors thank Nausheena Baig, Divya Donthi (USDA-ARS), and Johnny Magana (Paramount Farming) for their valuable contributions. Research was conducted under USDA-ARS CRIS Project 5325-42000-037-00D, TFCA 5325-42000-037-05 with the Almond Board of California, TFCA 5325-42000-037-07 with the California Pistachio Research Board, and portions of RCA 5325-42000-037-13 with the California Department of Food and Agriculture. NR 20 TC 9 Z9 9 U1 5 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3900 EI 1876-7486 J9 PHYTOCHEM LETT JI Phytochem. Lett. PD FEB PY 2014 VL 7 BP 225 EP 230 DI 10.1016/j.phytol.2013.03.004 PG 6 WC Plant Sciences; Chemistry, Medicinal SC Plant Sciences; Pharmacology & Pharmacy GA 302CE UT WOS:000330578100047 ER PT J AU Cao, JJ Pasiakos, SM Margolis, LM Sauter, ER Whigham, LD McClung, JP Young, AJ Combs, GF AF Cao, Jay J. Pasiakos, Stefan M. Margolis, Lee M. Sauter, Edward R. Whigham, Leah D. McClung, James P. Young, Andrew J. Combs, Gerald F., Jr. TI Calcium homeostasis and bone metabolic responses to high-protein diets during energy deficit in healthy young adults: a randomized controlled trial SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID INDUCED WEIGHT-LOSS; OVERWEIGHT PREMENOPAUSAL WOMEN; NET ACID EXCRETION; FAT-FREE MASS; POSTMENOPAUSAL WOMEN; CALORIC RESTRICTION; MINERAL DENSITY; VITAMIN-D; INDUCED HYPERCALCIURIA; POTASSIUM BICARBONATE AB Background: Although consuming dietary protein above current recommendations during energy deficit (ED) preserves lean body mass, concerns have been raised regarding the effects of high-protein diets on bone health. Objective: The objective was to determine whether calcium homeostasis and bone turnover are affected by high-protein diets during weight maintenance (WM) and ED. Design: In a randomized, parallel-design, controlled trial of 32 men and 7 Women, volunteers were assigned diets providing protein at 0.8 [Recommended Dietary Allowance (RDA)], 1.6 (2 x RDA), or 2.4 (3 x RDA) g . kg(-1) . d(-1) for 31 d. Ten days of WM preceded 21 d of ED during which total daily ED was 40%, achieved by reduced dietary energy intake (similar to 30%) and increased physical activity (similar to 10%). The macronutrient composition (protein g . kg(-1) . d(-1) and % fat) was held constant from WM to ED. Calcium absorption (ratio of Ca-44 to Ca-42) and circulating indexes of bone turnover were determined at day 8 (WM) and day 29 (ED). Results: Regardless of energy state, mean (+/- SEM) urinary pH was lower (P < 0.05) at 2 x RDA (6.28 +/- 0.05) and 3 x RDA (6.23 +/- 0.06) than at the RDA (6.54 +/- 0.06). However, protein had no effect on either, urinary calcium excretion (P > 0.05) or the amount of calcium retained (P > 0.05). ED decreased serum insulin-like growth factor I concentrations and increased serum tartrate-resistant acid phosphatase and 25-hydroxyvitamin D concentrations (P < 0.01). Remaining markers of bone turnover and whole-body bone mineral density and content were not affected by either the protein level or ED (P > 0.05). Conclusion: These data demonstrate that short-term consumption of high-protein diets does not disrupt calcium homeostasis and is not detrimental to skeletal integrity. C1 [Cao, Jay J.; Whigham, Leah D.; Combs, Gerald F., Jr.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Pasiakos, Stefan M.; Margolis, Lee M.; McClung, James P.; Young, Andrew J.] US Army, Environm Med Res Inst, Mil Nutr Div, Natick, MA 01760 USA. [Sauter, Edward R.] Univ N Dakota, Sch Med & Hlth Sci, Grand Forks, ND 58201 USA. RP Cao, JJ (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58202 USA. EM jay.cao@ars.usda.gov RI McClung, James/A-1989-2009; Pasiakos, Stefan/E-6295-2014; Biguzzi, Felipe/E-4724-2015 OI Pasiakos, Stefan/0000-0002-5378-5820; FU US Army Medical Research and Material Command; USDA Agricultural Research Service program "Bone Metabolism in Obesity" Current Research Information System [5450-51000-046-00D] FX Supported by the US Army Medical Research and Material Command and the USDA Agricultural Research Service program "Bone Metabolism in Obesity" Current Research Information System (no. 5450-51000-046-00D) as part of the authors' official duties. NR 62 TC 9 Z9 9 U1 0 U2 14 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD FEB PY 2014 VL 99 IS 2 BP 400 EP 407 DI 10.3945/ajcn.113.073809 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 295XR UT WOS:000330150200021 PM 24284444 ER PT J AU Rhee, MS Wei, LS Sawhney, N Rice, JD St John, FJ Hurlbert, JC Preston, JF AF Rhee, Mun Su Wei, Lusha Sawhney, Neha Rice, John D. St. John, Franz J. Hurlbert, Jason C. Preston, James F. TI Engineering the Xylan Utilization System in Bacillus subtilis for Production of Acidic Xylooligosaccharides SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID HYDROLASE FAMILY 30; SP STRAIN JDR-2; GLUCURONOXYLAN XYLANOHYDROLASE; ERWINIA-CHRYSANTHEMI; PENTOSAN POLYSULFATE; PROTEIN SECRETION; DEPOLYMERIZATION; HEMICELLULOSE; ENDOXYLANASES; INDUSTRIAL AB Xylans are the predominant polysaccharides in hemicelluloses and an important potential source of biofuels and chemicals. The ability of Bacillus subtilis subsp. subtilis strain 168 to utilize xylans has been ascribed to secreted glycoside hydrolase family 11 (GH11) and GH30 endoxylanases, encoded by the xynA and xynC genes, respectively. Both of these enzymes have been defined with respect to structure and function. In this study, the effects of deletion of the xynA and xynC genes, individually and in combination, were evaluated for xylan utilization and formation of acidic xylooligosaccharides. Parent strain 168 depolymerizes methylglucuronoxylans (MeGX(n)), releasing the xylobiose and xylotriose utilized for growth and accumulating the aldouronate methylglucuronoxylotriose (MeGX(3)) with some methylglucuronoxylotetraose (MeGX(4)). The combined GH11 and GH30 activities process the products generated by their respective actions on MeGXn to release a maximal amount of neutral xylooligosaccharides for assimilation and growth, at the same time forming MeGX(3) in which the internal xylose is substituted with methylglucuronate (MeG). Deletion of xynA results in the accumulation of beta-1,4-xylooligosaccharides with degrees of polymerization ranging from 4 to 18 and an average degree of substitution of 1 in 7.2, each with a single MeG linked alpha-1,2 to the xylose penultimate to the xylose at the reducing terminus. Deletion of the xynC gene results in the accumulation of aldouronates comprised of 4 or more xylose residues in which the MeG may be linked alpha-1,2 to the xylose penultimate to the nonreducing xylose. These B. subtilis lines may be used for the production of acidic xylooligosaccharides with applications in human and veterinary medicine. C1 [Rhee, Mun Su; Wei, Lusha; Sawhney, Neha; Rice, John D.; Preston, James F.] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA. [St. John, Franz J.] USDA, Forest Prod Lab, United States Forest Serv, Madison, WI 53705 USA. [Hurlbert, Jason C.] Winthrop Univ, Dept Chem Phys & Geol, Rock Hill, SC 29733 USA. RP Preston, JF (reprint author), Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA. EM jpreston@ufl.edu RI St John, Franz/J-8970-2016 OI St John, Franz/0000-0003-3458-5628 FU Biomass Research & Development Initiative [2011-10006-30358]; USDA National Institute of Food and Agriculture; Florida Energy Systems Consortium, State University System of Florida [00077818] FX This project was supported in part by Biomass Research & Development Initiative competitive grant no. 2011-10006-30358 from the USDA National Institute of Food and Agriculture and by the Florida Energy Systems Consortium, State University System of Florida, project no. 00077818. NR 45 TC 10 Z9 10 U1 1 U2 16 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD FEB PY 2014 VL 80 IS 3 BP 917 EP 927 DI 10.1128/AEM.03246-13 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 291SF UT WOS:000329848900013 PM 24271172 ER PT J AU Whitaker, WB Richards, GP Boyd, EF AF Whitaker, W. Brian Richards, Gary P. Boyd, E. Fidelma TI Loss of Sigma Factor RpoN Increases Intestinal Colonization of Vibrio parahaemolyticus in an Adult Mouse Model SO INFECTION AND IMMUNITY LA English DT Article ID III SECRETION SYSTEMS; FLAGELLAR GENE SYSTEM; ESCHERICHIA-COLI; MOLECULAR ANALYSIS; VIRULENCE FACTOR; CHOLERAE; MOTILITY; EXPRESSION; OYSTERS; WATER C1 [Whitaker, W. Brian; Boyd, E. Fidelma] Univ Delaware, Dept Biol Sci, Newark, DE 19716 USA. [Richards, Gary P.] Delaware State Univ, Agr Res Serv, USDA, Delaware, DE USA. RP Boyd, EF (reprint author), Univ Delaware, Dept Biol Sci, Newark, DE 19716 USA. EM fboyd@udel.edu FU NCRR NIH HHS [5P30RR031160-03, P30 RR031160]; NIGMS NIH HHS [P30 GM103519, 8 P30 GM103519-03] NR 69 TC 9 Z9 12 U1 0 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 EI 1098-5522 J9 INFECT IMMUN JI Infect. Immun. PD FEB PY 2014 VL 82 IS 2 BP 544 EP 556 DI 10.1128/IAI.01210-13 PG 13 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 298XF UT WOS:000330357100008 PM 24478070 ER PT J AU Brown, P Gipson, C AF Brown, Patricia Gipson, Chester TI A word from OLAW and USDA SO LAB ANIMAL LA English DT Editorial Material C1 [Brown, Patricia] NIH, OLAW, OER, HHS, Bethesda, MD 20892 USA. [Gipson, Chester] USDA, APHIS, AC, Washington, DC USA. RP Brown, P (reprint author), NIH, OLAW, OER, HHS, Bethesda, MD 20892 USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 EI 1548-4475 J9 LAB ANIMAL JI Lab Anim. PD FEB PY 2014 VL 43 IS 2 BP 55 EP 55 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA 297MJ UT WOS:000330259300017 PM 24451358 ER PT J AU Quito-Avila, DF Lightle, D Martin, RR AF Quito-Avila, Diego F. Lightle, Danielle Martin, Robert R. TI Effect of Raspberry bushy dwarf virus, Raspberry leaf mottle virus, and Raspberry latent virus on Plant Growth and Fruit Crumbliness in 'Meeker' Red Raspberry SO PLANT DISEASE LA English DT Article ID MOVEMENT PROTEIN GENES; CUCUMBER-MOSAIC-VIRUS; CORN LETHAL NECROSIS; SYNERGISTIC INTERACTIONS; POTATO-VIRUS; SWEET-POTATO; RNA; IDENTIFICATION; POTYVIRUS; RUBUS AB Raspberry crumbly fruit in red raspberry (Rubus idaeus), widespread in the Pacific Northwest of the United States and British Columbia, Canada, is most commonly caused by a virus infection. Raspberry bushy dwarf virus (RBDV) has long been attributed as the causal agent of the disease. Recently, the identification of two additional viruses, Raspberry leaf mottle virus (RLMV) and Raspberry latent virus (RpLV), in northern Washington and British Columbia, suggested the existence of a possible new virus complex responsible for the increased severity of the disease. Virus testing of crumbly fruited plants from five fields in northern Washington revealed the presence of RLMV and RpLV, in addition to RBDV. Plants with less severe crumbly fruit symptoms had a much lower incidence of RLMV or RpLV. Field trials using replicated plots of 'Meeker' plants containing single and mixed infections of RBDV, RLMV, or RpLV, along with a virus-free control, were developed to determine the role of RLMV and RpLV in crumbly fruit. Field evaluations during establishment and two fruiting years revealed that plants infected with the three viruses or the combinations RBDV+RLMV and RBDV+RpLV had the greatest reduction in cane growth, or fruit firmness and fruit weight, respectively. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) showed that the titer of RBDV was increased similar to 400-fold when it occurred in mixed infections with RLMV compared to RBDV in single infections. In addition, a virus survey revealed that RLMV and RpLV are present at high incidence in northern Washington; whereas the incidence in southern Washington and Oregon, where crumbly fruit is not as serious a problem, was considerably lower. C1 [Quito-Avila, Diego F.] CIBE ESPOL, Escuela Super Politecn Litoral, Ctr Invest Biotecnol Ecuador, Guayaquil, Ecuador. [Lightle, Danielle; Martin, Robert R.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. RP Quito-Avila, DF (reprint author), CIBE ESPOL, Escuela Super Politecn Litoral, Ctr Invest Biotecnol Ecuador, Guayaquil, Ecuador. EM diego.quito.avila@gmail.com FU United States Department of Agriculture under the Specialty Crops Research Initiative (SCRI) [2009-51181-06022, CRIS 5358-22000-032-00D] FX This study was partially funded by the United States Department of Agriculture under the Specialty Crops Research Initiative (SCRI), award number 2009-51181-06022, CRIS 5358-22000-032-00D. We thank people of the North-western Research and Extension Center in Mt. Vernon, Washington, particularly Gary Moulton and Tom Walters for their unconditional support in the execution of field work. Likewise, we express our special grattitude to raspberry growers in Washington and Oregon, especially Randy Hancoop and Rader's Farms for allowing us to use their fields to conduct surveys and virus monitoring. NR 45 TC 4 Z9 4 U1 0 U2 20 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2014 VL 98 IS 2 BP 176 EP 183 DI 10.1094/PDIS-05-13-0562-RE PG 8 WC Plant Sciences SC Plant Sciences GA 294XM UT WOS:000330081200001 ER PT J AU Saito, S Cadle-Davidson, L Wilcox, WF AF Saito, Seiya Cadle-Davidson, Lance Wilcox, Wayne F. TI Selection, Fitness, and Control of Grape Isolates of Botrytis cinerea Variably Sensitive to Fenhexamid SO PLANT DISEASE LA English DT Article ID POLYMERASE-CHAIN-REACTION; BOTRYOTINIA-FUCKELIANA; FUNGICIDE RESISTANCE; FRENCH VINEYARDS; GENETIC-ANALYSIS; 1ST REPORT; QUANTIFICATION; HYDROXYANILIDE; ANILINOPYRIMIDINE; PHENYLPYRROLE AB Of 683 Botrytis cinerea isolates collected from a fungicide-trial vineyard, 31 were classified as putatively resistant to fenhexamid (50% effective concentration [EC50] >= 0.1 mu g/ml). For the resistant isolates that survived and sporulated in culture, colony expansion and conidial germination frequency was significantly reduced relative to the mean of 30 representative baseline isolates (EC50 = 0.03 mu g/ml). Grape berries were inoculated with four isolates representing a range of fenhexamid sensitivities and treated preventively or curatively with fenhexamid concentrations (150 to 600 mg/liter) representing 25 to 100% of the recommended rate. All treatments significantly delayed disease onset and progress caused by isolates with EC50 values of 0.03 and 0.15 mu g/ml but provided little to no control of isolates with EC50 values of 0.32 and 62.5 mu g/ml. The latter isolate exhibited a previously unreported F427V mutation of ERG27, an enzyme of ergosterol biosynthesis. In a duplex quantitative polymerase chain reaction test, the ratio of pathogen/host DNA increased significantly for 14 days after inoculation of untreated berries with a baseline isolate but declined slightly in berries treated with fenhexamid at 600 mg/liter 1 day post inoculation. In the vineyard, disease control was affected by the number and rate of fenhexamid applications but B. cinerea isolates with EC50 >= 0.1 mu g/ml were not preferentially selected. C1 [Saito, Seiya] USDA ARS, San Joaquin Valley Agr Sci Ctr, Commod Protect & Qual Res Unit, Parlier, CA 93648 USA. [Cadle-Davidson, Lance] Cornell Univ, USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. [Wilcox, Wayne F.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, New York State Agr Expt Stn, Geneva, NY 14456 USA. RP Saito, S (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Commod Protect & Qual Res Unit, Parlier, CA 93648 USA. EM saitoseiya@hotmail.com NR 30 TC 5 Z9 5 U1 2 U2 17 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2014 VL 98 IS 2 BP 233 EP 240 DI 10.1094/PDIS-07-13-0746-RE PG 8 WC Plant Sciences SC Plant Sciences GA 294XM UT WOS:000330081200008 ER PT J AU Sikdar, P Okubara, P Mazzola, M Xiao, CL AF Sikdar, P. Okubara, P. Mazzola, M. Xiao, C. L. TI Development of PCR Assays for Diagnosis and Detection of the Pathogens Phacidiopycnis washingtonensis and Sphaeropsis pyriputrescens in Apple Fruit SO PLANT DISEASE LA English DT Article ID REAL-TIME; QUANTITATIVE PCR; PEAR FRUIT; ROT; STATE AB Speck rot caused by Phacidiopycnis washingtonensis and Sphaeropsis rot caused by Sphaeropsis pyriputrescens are two recently reported postharvest diseases of apple. Infection by these two pathogens occurs in the orchard but remains latent before harvest. Symptoms develop after harvest and are similar to those of gray mold caused by Botrytis cinerea. Accurate diagnosis of these diseases is important during the fruit inspection process, particularly in the instance of fruit destined for export. Early near-harvest detection of latent infections in apple fruit is an important step to implement relevant pre- and postharvest measures for disease control. The aim of this study was to develop polymerase chain reaction (PCR) assays for diagnosis and early detection of latent infections of apple fruit by P. washingtonensis and S. pyriputrescens. Species-specific primers based on the ribosomal DNA internal transcribed spacer region were designed for use in PCR assays. Conventional and real-time PCR assays were developed and validated using fruit inoculated with P washingtonensis, S. pyriputrescens, or B. cinerea and compared with identifications using traditional isolation-based assays. For wound-inoculated fruit, the PCR assays consistently provided the correct identification of the pathogen used as the inoculant in 6 h of processing time, compared with 5 to 6 days using culture-based methods. Real-time PCR assays effectively detected latent infections in symptomless stem and calyx tissues of fruit that were inoculated with the pathogens in the orchard during the growing season. The PCR assays provide a rapid, accurate method for diagnosis and early detection of these diseases. C1 [Sikdar, P.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Okubara, P.] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. [Mazzola, M.] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Xiao, C. L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Xiao, CL (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM Chang-Lin.Xiao@ars.usda.gov NR 21 TC 3 Z9 3 U1 0 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2014 VL 98 IS 2 BP 241 EP 246 DI 10.1094/PDIS-05-13-0495-RE PG 6 WC Plant Sciences SC Plant Sciences GA 294XM UT WOS:000330081200009 ER PT J AU Scott, JB Gent, DH Pethybridge, SJ Hay, FS AF Scott, Jason B. Gent, David H. Pethybridge, Sarah J. Hay, Frank S. TI Spatiotemporal Characterization of Sclerotinia Crown Rot Epidemics in Pyrethrum SO PLANT DISEASE LA English DT Article ID LETTUCE DROP; SPATIAL HIERARCHY; DISEASE INCIDENCE; RISK-FACTORS; PATTERN; AUSTRALIA; DYNAMICS; TASMANIA AB Sclerotinia crown rot, caused by Sclerotinia minor and S. sclerotiorum, is a disease of pyrethrum in Australia that may cause substantial decline in plant density. The spatiotemporal characteristics of the disease were quantified in 14 fields during three growing seasons. Fitting the binary power law to disease incidence provided slope (b = 1.063) and intercept (ln(A(p)) = 0.669) estimates significantly (P <= 0.0001) greater than 1 and 0, respectively, indicating spatial aggregation at the sampling unit scale that was dependent upon disease incidence. Covariate analyses indicated that application of fungicides did not significantly influence these estimates. Spatial autocorrelation and spatial analysis by distance indices indicated that spatial aggregation above the sampling unit scale was limited to 20 and 17% of transects analyzed, respectively. The range of significant aggregation was limited primarily to neighboring sampling units only. Simple temporal disease models failed to adequately describe disease progress, due to a decline in disease incidence in spring. The relationships between disease incidence at the scales of individual plants within quadrats and quadrats within a field was modeled using four predictors of sample size. The choice of the specific incidence incidence relationship influenced the classification of disease incidence as greater than or less than 2% of plants, a provisional commercial threshold for fungicide application. Together, these studies indicated that epidemics of Sclerotinia crown rot were dominated by small-scale aggregation of disease. Larger scale patterns of diseased plants, when present, were associated with severe disease outbreaks. The spatial and temporal analyses were suggestive of disease epidemics being associated with localized primary inoculum and other factors that favor disease development at a small scale. C1 [Scott, Jason B.; Hay, Frank S.] Univ Tasmania, Tasmanian Inst Agr, Burnie, Tas 7320, Australia. [Gent, David H.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Pethybridge, Sarah J.] New Zealand Inst Plant & Food Res Ltd, Christchurch, New Zealand. RP Scott, JB (reprint author), Univ Tasmania, Tasmanian Inst Agr, Cradle Coast Campus, Burnie, Tas 7320, Australia. EM Jason.Scott@utas.edu.au RI Scott, Jason/D-4553-2011; Scott, Jason/C-2395-2014 OI Scott, Jason/0000-0001-9443-4384; Scott, Jason/0000-0001-9443-4384 FU Botanical Resources Australia-Agricultural Services Pty Ltd. (BRA); United States Department of Agriculture-Agricultural Research Service [CRIS 5358-21000-035-00]; Australian Research Council (ARC) as part of the ARC-Linkage program [LP100100529] FX Funding for this research was provided by the Botanical Resources Australia-Agricultural Services Pty Ltd. (BRA), the United States Department of Agriculture-Agricultural Research Service CRIS 5358-21000-035-00, and the Australian Research Council (ARC) as part of the ARC-Linkage program (project LP100100529). We thank C. Palmer (Tasmanian Institute of Agriculture) for excellent technical assistance during the course of this study, G. Hughes (Scotland's Rural College) and M. Twomey (Oregon State University) for their review and helpful suggestions, and the field staff and growers of BRA for providing access to commercial fields. NR 30 TC 1 Z9 1 U1 0 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2014 VL 98 IS 2 BP 267 EP 274 DI 10.1094/PDIS-07-13-0692-RE PG 8 WC Plant Sciences SC Plant Sciences GA 294XM UT WOS:000330081200012 ER PT J AU Serrato-Diaz, LM Rivera-Vargas, LI Goenaga, R French-Monar, RD AF Serrato-Diaz, L. M. Rivera-Vargas, L. I. Goenaga, R. French-Monar, R. D. TI First Report of Lasiodiplodia theobromae Causing Inflorescence Blight and Fruit Rot of Longan (Dimocarpus longan L.) in Puerto Rico. SO PLANT DISEASE LA English DT News Item C1 [Serrato-Diaz, L. M.; French-Monar, R. D.] Texas A&M AgriLife Extens Serv, Dept Plant Pathol & Microbiol, Amarillo, TX 79106 USA. [Rivera-Vargas, L. I.] Univ Puerto Rico, Dept Crops & Agroenvironm Sci, Mayaguez, PR USA. [Goenaga, R.] USDA ARS, Trop Agr Res Stn, Mayaguez, PR USA. RP Serrato-Diaz, LM (reprint author), Texas A&M AgriLife Extens Serv, Dept Plant Pathol & Microbiol, Amarillo, TX 79106 USA. NR 4 TC 1 Z9 2 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2014 VL 98 IS 2 BP 279 EP 279 DI 10.1094/PDIS-05-13-0473-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 294XM UT WOS:000330081200023 ER PT J AU Damayanti, TA Alabi, OJ Hidayat, SH Crosslin, JM Naidu, RA AF Damayanti, T. A. Alabi, O. J. Hidayat, S. H. Crosslin, J. M. Naidu, R. A. TI First Report of Potato virus Y in Potato in West Java, Indonesia SO PLANT DISEASE LA English DT News Item C1 [Damayanti, T. A.; Hidayat, S. H.] Bogor Agr Univ, Fac Agr, Dept Plant Protect, Bogor 16680, Indonesia. [Alabi, O. J.; Naidu, R. A.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Dept Plant Pathol, Prosser, WA 99350 USA. [Crosslin, J. M.] USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. RP Damayanti, TA (reprint author), Bogor Agr Univ, Fac Agr, Dept Plant Protect, Bogor 16680, Indonesia. OI Alabi, Olufemi/0000-0002-2471-7052 NR 3 TC 2 Z9 2 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2014 VL 98 IS 2 BP 287 EP 287 DI 10.1094/PDIS-07-13-0745-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 294XM UT WOS:000330081200045 ER PT J AU Sorensen, I Fei, ZJ Andreas, A Willats, WGT Domozych, DS Rose, JKC AF Sorensen, Iben Fei, Zhangjun Andreas, Amanda Willats, William G. T. Domozych, David S. Rose, Jocelyn K. C. TI Stable transformation and reverse genetic analysis of Penium margaritaceum: a platform for studies of charophyte green algae, the immediate ancestors of land plants SO PLANT JOURNAL LA English DT Article DE charophyte; green algae; Penium margaritaceum; pectin; cellulose; plant cell wall; plant evolution; technical advance ID AGROBACTERIUM-MEDIATED TRANSFORMATION; STRIGOSUM-LITTORALE COMPLEX; CHLAMYDOMONAS-REINHARDTII; ARTIFICIAL MICRORNAS; CELLULOSE SYNTHASE; EARLY EVOLUTION; GENOME REVEALS; ORIGIN; ARABIDOPSIS; DNA AB The charophyte green algae (CGA, Streptophyta, Viridiplantae) occupy a key phylogenetic position as the immediate ancestors of land plants but, paradoxically, are less well-studied than the other major plant lineages. This is particularly true in the context of functional genomic studies, where the lack of an efficient protocol for their stable genetic transformation has been a major obstacle. Observations of extant CGA species suggest the existence of some of the evolutionary adaptations that had to occur for land colonization; however, to date, there has been no robust experimental platform to address this genetically. We present a protocol for high-throughput Agrobacterium tumefaciens-mediated transformation of Penium margaritaceum, a unicellular CGA species. The versatility of Penium as a model for studying various aspects of plant cell biology and development was illustrated through non-invasive visualization of protein localization and dynamics in living cells. In addition, the utility of RNA interference (RNAi) for reverse genetic studies was demonstrated by targeting genes associated with cell wall modification (pectin methylesterase) and biosynthesis (cellulose synthase). This provided evidence supporting current models of cell wall assembly and inter-polymer interactions that were based on studies of land plants, but in this case using direct observation in vivo. This new functional genomics platform has broad potential applications, including studies of plant organismal biology and the evolutionary innovations required for transition from aquatic to terrestrial habitats. C1 [Sorensen, Iben; Rose, Jocelyn K. C.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Fei, Zhangjun] Cornell Univ, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Andreas, Amanda; Domozych, David S.] Skidmore Coll, Dept Biol, Saratoga Springs, NY 12866 USA. [Andreas, Amanda; Domozych, David S.] Skidmore Coll, Skidmore Microscopy Imaging Ctr, Saratoga Springs, NY 12866 USA. [Willats, William G. T.] Univ Copenhagen, Dept Plant & Environm Sci, DK-1871 Copenhagen, Denmark. RP Rose, JKC (reprint author), Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. EM jr286@cornell.edu OI Willats, William/0000-0003-2064-4025 FU US Department of Energy [DOE-FOA 10-0000368]; US National Science Foundation [NSF-MCB 0919925, NSF-DBI 0922805]; Villum Kann Rasmussen Foundation, Denmark FX We thank Stephen Snyder, Jeffrey Doyle, Karl Niklas and Eliel Ruiz-May for technical support and helpful discussion. The JIM5 monoclonal antibody was obtained from Plant Probes (Leeds, UK). This work was supported by grants from the US Department of Energy to J.K.C.R. (DOE-FOA 10-0000368) and from the US National Science Foundation to D.S.D. (NSF-MCB 0919925 and NSF-DBI 0922805). I.S. was supported by an individual post-doctoral stipend from the Villum Kann Rasmussen Foundation, Denmark. NR 57 TC 17 Z9 17 U1 2 U2 42 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD FEB PY 2014 VL 77 IS 3 BP 339 EP 351 DI 10.1111/tpj.12375 PG 13 WC Plant Sciences SC Plant Sciences GA 294WQ UT WOS:000330079000001 PM 24308430 ER PT J AU Mandy, DE Goldford, JE Yang, H Allen, DK Libourel, IGL AF Mandy, Dominic E. Goldford, Joshua E. Yang, Hong Allen, Doug K. Libourel, Igor G. L. TI Metabolic flux analysis using C-13 peptide label measurements SO PLANT JOURNAL LA English DT Article DE metabolic flux analysis; isotopic labeling; subcellular compartmentation; proteomics; primary metabolism; orbital trap; technical advance ID BRASSICA-NAPUS EMBRYOS; BIDIRECTIONAL REACTION STEPS; DEVELOPING SOYBEAN EMBRYOS; STEADY-STATE; MASS-SPECTROMETRY; OPTIMAL-DESIGN; AMINO-ACIDS; NETWORKS; PLANTS; SEEDS AB C-13 metabolic flux analysis (MFA) has become the experimental method of choice to investigate the cellular metabolism of microbes, cell cultures and plant seeds. Conventional steady-state MFA utilizes isotopic labeling measurements of amino acids obtained from protein hydrolysates. To retain spatial information in conventional steady-state MFA, tissues or subcellular fractions must be dissected or biochemically purified. In contrast, peptides retain their identity in complex protein extracts, and may therefore be associated with a specific time of expression, tissue type and subcellular compartment. To enable single-sample' spatially and temporally resolved steady-state flux analysis, we investigated the suitability of peptide mass distributions (PMDs) as an alternative to amino acid label measurements. PMDs are the discrete convolution of the mass distributions of the constituent amino acids of a peptide. We investigated the requirements for the unique deconvolution of PMDs into amino acid mass distributions (AAMDs), the influence of peptide sequence length on parameter sensitivity, and how AAMD and flux estimates that are determined through deconvolution compare to estimates from a conventional GC-MS measurement-based approach. Deconvolution of PMDs of the storage protein -conglycinin of soybean (Glycine max) resulted in good AAMD and flux estimates if fluxes were directly fitted to PMDs. Unconstrained deconvolution resulted in inferior AAMD and flux estimates. PMD measurements do not include amino acid backbone fragments, which increase the information content in GC-MS-derived analyses. Nonetheless, the resulting flux maps were of comparable quality due to the precision of Orbitrap quantification and the larger number of peptide measurements. C1 [Mandy, Dominic E.; Goldford, Joshua E.; Yang, Hong; Libourel, Igor G. L.] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA. [Goldford, Joshua E.; Yang, Hong; Libourel, Igor G. L.] Univ Minnesota, BioTechnol Inst, St Paul, MN 55108 USA. [Allen, Doug K.] Donald Danforth Plant Sci Ctr, USDA ARS, Plant Genet Res Unit, St Louis, MO 63132 USA. RP Allen, DK (reprint author), Donald Danforth Plant Sci Ctr, USDA ARS, Plant Genet Res Unit, St Louis, MO 63132 USA. EM Doug.Allen@ars.usda.gov; Libourel@umn.edu RI Allen, Doug/M-2836-2013 OI Allen, Doug/0000-0001-8599-8946 FU US National Science Foundation [NSF/MCB-1042335, EF-1105249]; ONR [N000141310552]; United States Department of Agriculture Agricultural Research Service FX The authors acknowledge James Gierse (United States Department of Agriculture, Agricultural Research Service located at the Donald Danforth Plant Science Center, St. Louis, MO, USA) and the Donald Danforth Plant Science Center Proteomics and Mass Spectrometry facility as well as the Minnesota Supercomputing Institute. The authors received funding support from the US National Science Foundation (NSF/MCB-1042335 and EF-1105249), ONR (N000141310552), and the United States Department of Agriculture Agricultural Research Service. Any product or trademark mentioned here does not imply a warranty, guarantee, or endorsement by the U.S. Department of Agriculture over other suitable products. NR 57 TC 14 Z9 14 U1 3 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD FEB PY 2014 VL 77 IS 3 BP 476 EP 486 DI 10.1111/tpj.12390 PG 11 WC Plant Sciences SC Plant Sciences GA 294WQ UT WOS:000330079000012 PM 24279886 ER PT J AU Arita, S Tanaka, K AF Arita, Shawn Tanaka, Kiyoyasu TI Heterogeneous multinational firms and productivity gains from falling FDI barriers SO REVIEW OF WORLD ECONOMICS LA English DT Article DE Multinational firms; FDI; Firm heterogeneity; Investment liberalization ID INTERNATIONAL-TRADE; EXPORTS; PLANTS AB This paper quantitatively assesses the impact of falling foreign direct investment (FDI) barriers on individual firms and its implications for intra-industry reallocation and aggregate productivity. We calibrate the firm-heterogeneity model of Eaton et al. (Econometrica 79(5):1453-1498, 2011) to match micro-level data on Japanese multinational firms facing fixed and variable costs of foreign production. We demonstrate that the calibrated model can be used to replicate the entry and sales patterns of Japanese multinationals. Counterfactual simulations show that declining FDI barriers lead to a disproportionate expansion of foreign production by more efficient firms relative to less efficient firms. A hypothetical 20 % reduction in FDI barriers is found to generate up to a 26.8 % improvement in industry-level productivity through global market-share reallocations within the industry. Compared with fixed entry barriers, reallocation effects and productivity gains are larger for a reduction of variable costs of foreign production. C1 [Arita, Shawn] Econ Res Serv, USDA, Washington, DC 20520 USA. [Tanaka, Kiyoyasu] Inst Developing Econ, Mihama Ku, Chiba 2618545, Japan. RP Arita, S (reprint author), Econ Res Serv, USDA, 1400 Independence Ave SW,Mailstop 1800, Washington, DC 20520 USA. EM ssarita@ers.usda.gov; kiyoyasu_tanaka@ide.go.jp NR 30 TC 2 Z9 2 U1 6 U2 12 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1610-2878 EI 1610-2886 J9 REV WORLD ECON JI Rev. World Econ. PD FEB PY 2014 VL 150 IS 1 BP 83 EP 113 DI 10.1007/s10290-013-0174-1 PG 31 WC Economics; International Relations SC Business & Economics; International Relations GA 296HQ UT WOS:000330176300004 ER PT J AU Cabrera, AR Shirk, PD Teal, PEA Grozinger, CM Evans, JD AF Cabrera, Ana R. Shirk, Paul D. Teal, Peter E. A. Grozinger, Christina M. Evans, Jay D. TI EXAMINING THE ROLE OF foraging AND malvolio IN HOST-FINDING BEHAVIOR IN THE HONEY BEE PARASITE, Varroa destructor (ANDERSON & TRUEMAN) SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE varroasis; ectoparasite; cGMP-dependent protein kinase; ion transporter; Apis mellifera ID DEPENDENT PROTEIN-KINASE; DIVISION-OF-LABOR; APIS-MELLIFERA; ECTOPARASITIC MITE; TASTE BEHAVIOR; DROSOPHILA; GENE; EXPRESSION; HOMOLOG; MELANOGASTER AB When a female varroa mite, Varroa destructor (Anderson & Trueman), invades a honey bee brood cell, the physiology rapidly changes from feeding phoretic to reproductive. Changes in foraging and malvolio transcript levels in the brain have been associated with modulated intra-specific food searching behaviors in insects and other invertebrates. Transcription profiles for both genes were examined during and immediately following brood cell invasion to assess their role as potential control elements. Vdfor and Vdmvl transcripts were found in all organs of varroa mites with the highest Vdfor transcript levels in ovary-lyrate organs and the highest Vdmvl in Malpighian tubules. Changes in transcript levels of Vdfor and Vdmvl in synganglia were not associated with the cell invasion process, remaining comparable between early reproductive mites (collected from the pre-capping brood cells) and phoretic mites. However, Vdfor and Vdmvl transcript levels were lowered by 37 and 53%, respectively, in synganglia from reproductive mites compared to early reproductive mites, but not significantly different to levels in synganglia from phoretic mites. On the other hand, in whole body preparations the Vdfor and Vdmvl had significantly higher levels of transcript in reproductive mites compared to phoretic and early reproductive, mainly due to the presence of both transcripts accumulating in the eggs carried by the ovipositing mite. Varroa mites are a critical component for honey bee population decline and finding varroa mite genes associated with brood cell invasion, reproduction, ion balance and other physiological processes will facilitate development of novel control avenues for this honey bee parasite. C1 [Cabrera, Ana R.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Shirk, Paul D.; Teal, Peter E. A.] USDA ARS CMAVE, Gainesville, FL 32608 USA. [Grozinger, Christina M.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Evans, Jay D.] USDA ARS BRL, BARC E, Beltsville, MD USA. RP Shirk, PD (reprint author), USDA ARS CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM paul.shirk@ars.usda.gov RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 FU USDA-NIFA [2009-05254]; Pennsylvania State University FX The authors thank Dr. Kevin Donohue for access to the varroa mite transcriptome data base and Drs. Roger Huybrechts and Katherine Aronstein for their comments on the manuscript. In addition, we thank Rebecca Blair, Jordyn Christian, and Kaddie Hung for assistance in beekeeping, varroa mite collections, and sample processing, and Richard Furlong for his assistance in molecular cloning. There is no conflict of interest for any of the authors. The varroa genomic sequencing was supported by USDA-NIFA grant 2009-05254 (to J.D.E.) and the varroa transcriptome was partially supported by funds from Pennsylvania State University (to C. M. G.). The use of trade name, commercial product, or corporation in this publication is for the information and convenience of the reader and does not imply an official recommendation, endorsement, or approval by the U. S. Department of Agriculture or the Agricultural Research Service for any product or service to the exclusion of others that may be suitable. NR 37 TC 2 Z9 2 U1 5 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 EI 1520-6327 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD FEB PY 2014 VL 85 IS 2 BP 61 EP 75 DI 10.1002/arch.21143 PG 15 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA 291IQ UT WOS:000329822800002 PM 24375502 ER PT J AU Kim, WS Krishnan, HB AF Kim, Won-Seok Krishnan, Hari B. TI A nopA Deletion Mutant of Sinorhizobium fredii USDA257, a Soybean Symbiont, is Impaired in Nodulation SO CURRENT MICROBIOLOGY LA English DT Article ID III SECRETION SYSTEM; CULTIVAR-SPECIFIC NODULATION; SURFACE APPENDAGES; FUNCTIONAL-ANALYSIS; GENOME SEQUENCE; STRAIN NGR234; RHIZOBIUM; PROTEIN; ORGANIZATION; EXPRESSION AB Sinorhizobium fredii USDA257 employs type III secretion system (T3SS) to deliver effector proteins into the host cells through pili. The nopA protein is the major component of USDA257 pili. The promoter region of USDA257 nopA possesses a well conserved tts box. Serial deletion analysis revealed that the tts box is absolutely essential for flavonoid induction of nopA. Deletion of nopA drastically lowered the number of nodules formed by USDA257 on cowpea and soybean cultivar Peking. In contrast to the parental strain, the USDA257 nopA mutant was able to form few nodules on soybean cultivars McCall and Williams 82. Light and transmission electron microscopy examination of these nodules revealed numerous starch grains both in the infected and uninfected cells. C1 [Kim, Won-Seok; Krishnan, Hari B.] Univ Missouri, Plant Genet Res Unit, ARS, USDA, Columbia, MO 65211 USA. RP Krishnan, HB (reprint author), Univ Missouri, Plant Genet Res Unit, ARS, USDA, Columbia, MO 65211 USA. EM KrishnanH@missouri.edu NR 26 TC 2 Z9 2 U1 2 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0343-8651 EI 1432-0991 J9 CURR MICROBIOL JI Curr. Microbiol. PD FEB PY 2014 VL 68 IS 2 BP 239 EP 246 DI 10.1007/s00284-013-0469-4 PG 8 WC Microbiology SC Microbiology GA 293SR UT WOS:000329994500017 PM 24121614 ER PT J AU Bansal, R Mian, MAR Michel, AP AF Bansal, Raman Mian, M. A. Rouf Michel, Andy P. TI Microbiome diversity of Aphis glycines with extensive superinfection in native and invasive populations SO ENVIRONMENTAL MICROBIOLOGY REPORTS LA English DT Article ID ECOLOGICALLY IMPORTANT TRAITS; POLYMERASE-CHAIN-REACTION; SOYBEAN APHID; ACYRTHOSIPHON-PISUM; HEMIPTERA APHIDIDAE; AMINO-ACID; PEA APHID; BACTERIAL SYMBIONTS; NORTH-AMERICA; EVOLUTIONARY RELATIONSHIPS AB Associations among insects and microbes can lead to beneficial or parasitic interactions. Using 454 sequencing of 16S RNA genes, we compared microbiome diversity and abundance among field-collected (F) and laboratory-reared (L) populations of the invasive soybean aphid (Aphis glycines), a pest of soybean. Additionally, we screened A.glycines populations from native (Japan, South Korea and China) and invasive regions (North America) to broadly determine the microbiome diversity. Our results suggested that Arsenophonus (relative abundance of 54.6%), Buchnera (38.7%) and Wolbachia (3.7%) were the major bacteria associated with A.glycines. Arsenophonus was the most abundant in F populations but was significantly reduced in L populations; additional bacteria species also had lower relative abundances in L populations. Native and invasive populations were largely similar in bacteria communities and revealed substantial superinfection of Arsenophonus and Wolbachia. The lone exception was a lack of Arsenophonus in A.glycines from Japan. Divergent selection pressures among natural and laboratory populations were inferred as factors driving the differential bacterial communities observed. Our results will allow for improved comparative aphid-symbiont research and broaden our understanding of the interactions among insects, endosymbionts and their environments. C1 [Bansal, Raman; Mian, M. A. Rouf; Michel, Andy P.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, USDA ARS, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, Dept Hort & Crop Sci, Wooster, OH 44691 USA. RP Michel, AP (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, 1680 Madison Ave, Wooster, OH 44691 USA. EM michel.70@osu.edu FU Department of Entomology; Ohio Agricultural Research and Development Center; North Central Soybean Research Program; United Soybean Board; Ohio Soybean Council FX We are grateful to F.L. Meng (China), T. Murai (Japan), and S. Kang and J. Jung (South Korea) for providing us the soybean aphid samples from native habitats. We are thankful to Lucia Orantes, Wei Zhang and Jacob Wenger for performing DNA extractions for the screening of bacteria across native and invasive experiments. Funding was provided by the Department of Entomology, The Ohio Agricultural Research and Development Center, The North Central Soybean Research Program, The United Soybean Board, and The Ohio Soybean Council. We declare no conflicts of interest. NR 112 TC 13 Z9 13 U1 4 U2 45 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1758-2229 J9 ENV MICROBIOL REP JI Environ. Microbiol. Rep. PD FEB PY 2014 VL 6 IS 1 BP 57 EP 69 DI 10.1111/1758-2229.12108 PG 13 WC Environmental Sciences; Microbiology SC Environmental Sciences & Ecology; Microbiology GA 291IB UT WOS:000329820500007 PM 24596263 ER PT J AU Augustine, DJ Derner, JD AF Augustine, David J. Derner, Justin D. TI Controls over the strength and timing of fire-grazer interactions in a semi-arid rangeland SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE foraging behaviour; grazing distribution; patch burn grazing management; pyric herbivory; resource selection; shortgrass steppe; spatial heterogeneity ID TALLGRASS PRAIRIE; SHORTGRASS STEPPE; PYRIC-HERBIVORY; GRASSLAND BIRD; SPATIAL HETEROGENEITY; VEGETATION RESPONSES; GRAZING INTERACTION; BURNED PATCHES; GREAT-PLAINS; HABITAT AB The degree to which large herbivores select and forage within recently burned areas is a key driver of vegetation heterogeneity in rangeland ecosystems. However, few studies have quantified the strength and timing of herbivore selection for burned areas or examined how selection strength varies among ecosystems differing in precipitation and primary productivity. We conducted a 4-year patch-burning experiment in semi-arid rangeland of Colorado, USA, where 25% of the area available to cattle was burned each year and burned patches were shifted annually. We used GPS collars with activity sensors to quantify the distribution of free-ranging cattle at a high temporal resolution (5-min intervals) during the growing season each year. We used a classification tree model to discriminate between cattle grazing vs. non-grazing locations, which significantly increased precision in quantifying burn selection strength. We fit generalized linear models predicting the frequency of cattle use of a given location within each study area and month, enabling comparisons between the relative influence of burns and topography on grazing distribution. Across multiple growing seasons, cattle selectively spent 31% of grazing time on recently burnt areas, which comprised 25% of the landscape; this selection strength was half as strong as that documented in mesic rangeland. At a monthly temporal scale, strong cattle selection for burned areas occurred during periods of rapid vegetation growth regardless of when during the growing season this greening occurred. Outside these intervals, burn selection strength was inconsistent and cattle grazing distribution was primarily influenced by topography. Thus, the relative importance of fire and topography in controlling grazer distribution was temporally contingent upon the timing and size of precipitation pulses.Synthesis and applications. Spatiotemporal interactions between fire and herbivores are a consistent feature of both semi-arid and mesic rangelands, with interaction strength varying across gradients of precipitation and primary productivity. Management of semi-arid ecosystems to sustain ecological processes should include strategies that allow ungulate herbivores to shift their grazing distribution seasonally in response to fire, topoedaphic variation and precipitation patterns. Combined management of fire and grazing for conservation objectives can be consistent with, and even complementary to, livestock production goals. C1 [Augustine, David J.] ARS, Rangeland Resources Res Unit, USDA, Ft Collins, CO 80526 USA. [Derner, Justin D.] ARS, Rangeland Resources Res Unit, USDA, Cheyenne, WY 82009 USA. RP Augustine, DJ (reprint author), ARS, Rangeland Resources Res Unit, USDA, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM david.augustine@ars.usda.gov FU USDA - Agricultural Research Service FX Research was funded by the USDA - Agricultural Research Service. We thank Patrick McCusker, Jeff Thomas, Troy Smith, Mary Ashby and Matt Mortenson for managing and collaring cattle; David Smith and Carmen Kennedy for data base management, William Armstrong for GIS support and Crow Valley Livestock Cooperative for providing study cattle. NR 42 TC 13 Z9 13 U1 3 U2 69 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD FEB PY 2014 VL 51 IS 1 BP 242 EP 250 DI 10.1111/1365-2664.12186 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 291RJ UT WOS:000329846500026 ER PT J AU O'Sullivan, A Armstrong, P Schuster, GU Pedersen, TL Allayee, H Stephensen, CB Newman, JW AF O'Sullivan, Aifric Armstrong, Patrice Schuster, Gertrud U. Pedersen, Theresa L. Allayee, Hooman Stephensen, Charles B. Newman, John W. TI Habitual Diets Rich in Dark-Green Vegetables Are Associated with an Increased Response to omega-3 Fatty Acid Supplementation in Americans of African Ancestry SO JOURNAL OF NUTRITION LA English DT Article ID FISH-OIL SUPPLEMENTATION; CORONARY-HEART-DISEASE; POLYUNSATURATED FATTY-ACIDS; HEALTHY EATING INDEX-2005; LOW-DENSITY-LIPOPROTEIN; CARDIOVASCULAR-DISEASE; EICOSAPENTAENOIC ACID; 5-LIPOXYGENASE GENE; ARTERY-DISEASE; RISK-FACTORS AB Although substantial variation exists in individual responses to omega-3 (omega-3) (n-3) fatty acid supplementation, the causes for differences in response are largely unknown. Here we investigated the associations between the efficacy of omega-3 fatty acid supplementation and a broad range of nutritional and clinical factors collected during a double-blind, placebo-controlled trial in participants of African ancestry, randomly assigned to receive either 2 g eicosapentaenoic acid (EPA) + 1 g docosahexaenoic acid (n = 41) or corn/soybean oil placebo (n = 42) supplements for 6 wk. Food-frequency questionnaires were administered, and changes in erythrocyte lipids, lipoproteins, and monocyte 5-lipoxygenase-dependent metabolism were measured before and after supplementation. Mixed-mode linear regression modeling identified high (n = 28) and low (n = 13) omega-3 fatty acid response groups on the basis of changes in erythrocyte EPA abundance (P < 0.001). Compliance was equivalent (similar to 88%), whereas decreases in plasma triglycerides and VLDL particle sizes and reductions in stimulated monocyte leukotriene B-4 production were larger in the high-response group. Although total diet quality scores were similar, the low-response group showed lower estimated 2005 Healthy Eating Index subscores for dark-green and orange vegetables and legumes (P = 0.01) and a lower intake of vegetables (P = 0.02), particularly dark-green vegetables (P = 0.002). Because the findings reported here are associative in nature, prospective studies are needed to determine if dietary dark-green vegetables or nutrients contained in these foods can enhance the efficacy of omega-3 fatty acid supplements. This trial was registered at clinicaltrials.gov as NCT00536185. C1 [O'Sullivan, Aifric] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. [Armstrong, Patrice; Schuster, Gertrud U.; Stephensen, Charles B.; Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Pedersen, Theresa L.; Newman, John W.] ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, USDA, Davis, CA USA. [Stephensen, Charles B.] ARS, Immun & Dis Prevent Res Unit, Western Human Nutr Res Ctr, USDA, Davis, CA USA. [Allayee, Hooman] Univ So Calif, Keck Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA. [Allayee, Hooman] Univ So Calif, Keck Sch Med, Dept Prevent Med, Los Angeles, CA 90033 USA. RP Newman, JW (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. EM john.newman@arsusda.gov FU NIH [AT003411, HL079353, P60MD0222]; USDA [5306-51530-006-00D, 5306-51530-019-00D]; NIH National Center for Research Resources Research Facilities Improvement Program [RR10600-01, CA62528-01, RR14514-01]; Gustavus and Louise Pfeiffer Research Foundation fellowship FX Supported by the NIH (AT003411 to C.B.S., HL079353 to HA, P60MD0222 to the UC Davis Center of Excellence in Nutritional Genomics) and the USDA (Intramural Projects 5306-51530-006-00D to C.B.S. and 5306-51530-019-00D to J.W.N.). The NIH National Center for Research Resources Research Facilities Improvement Program (RR10600-01, CA62528-01, RR14514-01) supported the facility that was used for a portion of this work. P.A. was supported by a Gustavus and Louise Pfeiffer Research Foundation fellowship. NR 51 TC 7 Z9 7 U1 1 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD FEB PY 2014 VL 144 IS 2 BP 123 EP 131 DI 10.3945/jn.113.181875 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 294XF UT WOS:000330080500003 PM 24259553 ER PT J AU Du, XG Wang, JP Niu, XL Smith, D Wu, DY Meydani, SN AF Du, Xiaogang Wang, Junpeng Niu, Xinli Smith, Donald Wu, Dayong Meydani, Simin Nikbin TI Dietary Wolfberry Supplementation Enhances the Protective Effect of Flu Vaccine against Influenza Challenge in Aged Mice SO JOURNAL OF NUTRITION LA English DT Article ID LYCIUM-BARBARUM POLYSACCHARIDES; IMMUNE-RESPONSE; DENDRITIC CELLS; PROTEIN COMPLEX; UNITED-STATES; IMMUNOSENESCENCE; FORMULATION; POPULATION; MATURATION; INFECTION AB Current vaccines for influenza do not fully protect the aged against influenza infection. Although wolfberry (goji berry) has been shown to improve immune response, including enhanced antibody production, after vaccination in the aged, it is not known if this effect would translate to better protection after influenza infection, nor is its underlying mechanism well understood. To address these issues, we conducted a study using a 2 x 2 design in which aged male mice (20-22 mo) were fed a control or a 5% wolfberry diet for 30 d, then immunized with an influenza vaccine or saline (control) on days 31 and 52 of the dietary intervention, and finally challenged with influenza A/Puerto Rico/8/34 virus. Mice fed wolfberry had higher influenza antibody titers and improved symptoms (less postinfection weight loss) compared with the mice treated by vaccine alone. Furthermore, an in vitro mechanistic study showed that wolfberry supplementation enhanced maturation and activity of antigen-presenting dendritic cells (DCs) in aged mice, as indicated by phenotypic change in expression of DC activation markers major histocompatibility complex class II, cluster of differentiation.(CD) 40, CD80, and CD86, and functional change in DC production of cytokines interleukin-12 and tumor necrosis factor-alpha as well as DC endocytosis. Also, adoptive transfer of wolfberry-treated bone marrow DCs (loaded with ovalbumin(323-339)-peptide) promoted antigen-specific T cell proliferation as well as interleukin-4 and interferon-gamma production in CD4(+) T cells. In summary, our data indicate that dietary wolfberry enhances the efficacy of influenza vaccination, resulting in better host protection to prevent subsequent influenza infection; this effect may be partly attributed to improved DC function. C1 [Du, Xiaogang; Wang, Junpeng; Niu, Xinli; Wu, Dayong; Meydani, Simin Nikbin] Tufts Univ, Nutr Immunol Lab, Boston, MA 02111 USA. [Smith, Donald] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Comparat Biol Unit, Boston, MA 02111 USA. RP Wu, DY (reprint author), Tufts Univ, Nutr Immunol Lab, Boston, MA 02111 USA. EM dayong.wu@tufts.edu; simin.meydani@tufts.edu FU USDA/Agricultural Research Service [58-1950-0-014] FX Supported by the USDA/Agricultural Research Service under contract 58-1950-0-014. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. NR 38 TC 1 Z9 1 U1 2 U2 24 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD FEB PY 2014 VL 144 IS 2 BP 224 EP 229 DI 10.3945/jn.113.183566 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 294XF UT WOS:000330080500016 PM 24336457 ER PT J AU Manfredini, F Lucas, C Nicolas, M Keller, L Shoemaker, D Grozinger, CM AF Manfredini, Fabio Lucas, Christophe Nicolas, Michael Keller, Laurent Shoemaker, Dewayne Grozinger, Christina M. TI Molecular and social regulation of worker division of labour in fire ants SO MOLECULAR ECOLOGY LA English DT Article DE division of labour; fire ants; foraging workers; microarrays; queen pheromone; sociogenomics ID APIS-MELLIFERA L.; LARGE GENE LISTS; SOLENOPSIS-INVICTA; HONEY-BEES; FORAGING BEHAVIOR; INSECT SOCIETIES; QUEEN NUMBER; PHEROMONE; EXPRESSION; COLONIES AB Reproductive and worker division of labour (DOL) is a hallmark of social insect societies. Despite a long-standing interest in worker DOL, the molecular mechanisms regulating this process have only been investigated in detail in honey bees, and little is known about the regulatory mechanisms operating in other social insects. In the fire ant Solenopsis invicta, one of the most studied ant species, workers are permanently sterile and the tasks performed are modulated by the worker's internal state (age and size) and the outside environment (social environment), which potentially includes the effect of the queen presence through chemical communication via pheromones. However, the molecular mechanisms underpinning these processes are unknown. Using a whole-genome microarray platform, we characterized the molecular basis for worker DOL and we explored how a drastic change in the social environment (i.e. the sudden loss of the queen) affects global gene expression patterns of worker ants. We identified numerous genes differentially expressed between foraging and nonforaging workers in queenright colonies. With a few exceptions, these genes appear to be distinct from those involved in DOL in bees and wasps. Interestingly, after the queen was removed, foraging workers were no longer distinct from nonforaging workers at the transcriptomic level. Furthermore, few expression differences were detected between queenright and queenless workers when we did not consider the task performed. Thus, the social condition of the colony (queenless vs. queenright) appears to impact the molecular pathways underlying worker task performance, providing strong evidence for social regulation of DOL in S.invicta. C1 [Manfredini, Fabio; Grozinger, Christina M.] Penn State Univ, Dept Entomol, Ctr Pollinator Res, University Pk, PA 16802 USA. [Manfredini, Fabio] Royal Holloway Univ London, Sch Biol Sci, Egham TW20 0EX, Surrey, England. [Lucas, Christophe] Univ Tours, CNRS, Inst Rech Biol Insecte, UMR 7261, F-37200 Tours, France. [Lucas, Christophe; Nicolas, Michael; Keller, Laurent] Univ Lausanne, Dept Ecol & Evolut, Lausanne, Switzerland. [Shoemaker, Dewayne] USDA ARS, Gainesville, FL 32608 USA. RP Manfredini, F (reprint author), Penn State Univ, Dept Entomol, Ctr Pollinator Res, University Pk, PA 16802 USA. EM fmanfredini79@gmail.com RI Lucas, Christophe/A-4186-2011; OI Lucas, Christophe/0000-0003-2839-7583; keller, laurent/0000-0002-5046-9953 FU US Department of Agriculture AFRI Award [2009-35302-05301]; Swiss NSF; ERC; Societe Academique Vaudoise FX The authors would like to thank Sarah Kocher (Harvard) and Naomi Altman (Penn State) for assistance with the statistical analysis and Mark Brown, Elli Leadbeater, members of the Brown group and the three anonymous reviewers for providing thoughtful comments that helped improving the quality of the manuscript. The authors would also like to thank Alex McMenamin for performing the qRT-PCR studies. Many thanks to Craig Praul and his team at the Penn State Genomics Core Facility for performing all the steps to prepare RNA samples for microarrays and for providing assistance during RNA extraction and data analysis. A special thanks to Eileen Carroll, Chin-Cheng Yang (Scotty) and all the undergraduate research assistants in the Shoemaker Lab that provided extraordinary support, help and advice during field work and laboratory experiments at the USDA-ARS, CMAVE in Gainesville, Florida. This study was funded by US Department of Agriculture AFRI Award 2009-35302-05301 to DS, CMG and John Wang (Academia Sinica), the Swiss NSF and an ERC advanced grant to LK and by the Societe Academique Vaudoise to CL. NR 63 TC 8 Z9 8 U1 6 U2 79 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD FEB PY 2014 VL 23 IS 3 BP 660 EP 672 DI 10.1111/mec.12626 PG 13 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 293NW UT WOS:000329980000014 PM 24329612 ER PT J AU Qin, BL Panickar, KS Anderson, RA AF Qin, Bolin Panickar, Kiran S. Anderson, Richard A. TI Cinnamon polyphenols regulate S100 beta, sirtuins, and neuroactive proteins in rat C6 glioma cells SO NUTRITION LA English DT Article DE Polyphenols; S100 beta; Sirtuins; Phospho-NF-kappa B and Bcl-xl; Spice ID NF-KAPPA-B; CORTICAL ASTROCYTE CULTURES; INCREASES GLUTAMATE UPTAKE; IN-VITRO; S100B SECRETION; HIPPOCAMPAL-NEURONS; BIOLOGICAL-ACTIVITY; ALZHEIMERS-DISEASE; SIGNALING PATHWAYS; GROWTH-FACTOR AB Objective: Increasing evidence suggests that cinnamon has many health benefits when used in herbal medicine and as a dietary ingredient. The aim of this study was to investigate the effects of an aqueous extract of cinnamon, high in type A polyphenols, on molecular targets in rat C6 glioma cells that underlie their protective effects. Methods: C6 rat glioma cells were seeded in 35-mm culture dishes or six-well plates, then were incubated with cinnamon polyphenols at doses of 10 and 20 mu g/mL for 24 h. The targeting protein expression, secretion, and phosphorylation were evaluated by immunoprecitation/immunoblotting and immunofluorescence imaging. Results: Cinnamon polyphenols significantly enhanced secretion of S100 beta, a Ca2+-binding protein, and increased intracellular S100 beta expression after 24 h of incubation, in rat C6 glioma cells. Cinnamon polyphenols also enhanced protein levels of sirtuin 1, 2, and 3, deacetylases important in cell survival, and the tumor suppressor protein, p53, and inhibited the inflammatory factors, tumor necrosis factor alpha, and phospho-p65, a subunit of nuclear factor-kappa beta. Cinnamon polyphenols also up-regulated levels of phospho-p38, extracellular signal-regulated protein and mitogen-activated protein and kinase-activated protein kinases that may be important for prosurvival functions. Conclusion: Our results indicate that the effects of cinnamon polyphenols on upregulating prosurvival proteins, activating mitogen-activated protein kinase pathways, and decreasing proinflammatory cytokines may contribute to their neuroprotective effects. (C) 2014 Elsevier Inc. All rights reserved. C1 [Qin, Bolin; Panickar, Kiran S.; Anderson, Richard A.] USDA ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Qin, Bolin] Integr Nutraceut Int, Springhill, TN USA. [Panickar, Kiran S.] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA. RP Qin, BL (reprint author), USDA ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. EM Bolin.Qin@ars.usda.gov FU USDA Cooperative Research and Development Agreement (CRADA) [58-3K95-7-1184]; Integrity Nutraceuticals International, Spring Hill, TN FX This work was supported in part by a USDA Cooperative Research and Development Agreement (CRADA No.58-3K95-7-1184) with Integrity Nutraceuticals International, Spring Hill, TN. BQ has a joint appointment with the U.S. Department of Agriculture and Integrity Nutraceutical International (Spring Hill, TN). Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture is an equal opportunity provider and employer. NR 64 TC 2 Z9 2 U1 1 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0899-9007 EI 1873-1244 J9 NUTRITION JI Nutrition PD FEB PY 2014 VL 30 IS 2 BP 210 EP 217 DI 10.1016/j.nut.2013.07.001 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 293BZ UT WOS:000329947700014 PM 24239092 ER PT J AU Zhebentyayeva, TN Fan, SH Chandra, A Bielenberg, DG Reighard, GL Okie, WR Abbott, AG AF Zhebentyayeva, Tatyana N. Fan, Shenghua Chandra, Anirudh Bielenberg, Douglas G. Reighard, Gregory L. Okie, William R. Abbott, Albert G. TI Dissection of chilling requirement and bloom date QTLs in peach using a whole genome sequencing of sibling trees from an F-2 mapping population SO TREE GENETICS & GENOMES LA English DT Article DE Chilling requirement; Bloom date; QTL intervals; Whole genome sequencing; Illumina platform; Haplotype polymorphism ID MADS-BOX GENES; PRUNUS-PERSICA L.; SUPPRESSION SUBTRACTIVE HYBRIDIZATION; BUD DORMANCY RELEASE; ARABIDOPSIS-THALIANA; FLOWERING TIME; HISTONE MODIFICATIONS; EXPRESSION PATTERNS; JAPANESE APRICOT; ARMENIACA L. AB Chilling requirement (CR) for floral bud dormancy release is one of the major limiting factors for geographical adaptation of fruiting trees. Using a whole genome sequencing approach (Illumina platform), we explored polymorphism underlying phenotypic differences among individuals in a peach F-2 cross segregating for chilling requirement and bloom date. Allelic configuration of individuals, which represented phenotypic extremes in the cross (300 vs. 1,100 chill hours) allowed reconstruction of low- and high-chill haplotypes within three most significant quantitative trait locus (QTL) intervals on the Prunus G1, G4, and G7. We detected single nucleotide polymorphic sites (SNPs), small deletions and insertions (DIPs), and large structural variants (SVs) associated with low-chill haplotypes and created a prioritized list of candidate genes based on functionally characterized homologs from Arabidopsis thaliana. Two dormancy associated genes PpeDAM5 and PpeDAM6 are the strongest candidate genes for the major QTL signal at the lower end of G1. Also, key functional genes involved in the Polycomb repressive mechanism, cell cycle progression, and hormone regulation were evident as strong candidate genes underlying QTL intervals in this peach cross. C1 [Zhebentyayeva, Tatyana N.; Chandra, Anirudh; Abbott, Albert G.] Clemson Univ, Dept Biochem & Genet, Robert F Poole Agr Ctr, Clemson, SC 29634 USA. [Fan, Shenghua] Greenwood Genet Ctr, JC Self Res Inst Human Genet, Greenwood, SC 29646 USA. [Bielenberg, Douglas G.; Reighard, Gregory L.] Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC 29634 USA. [Okie, William R.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Abbott, Albert G.] Univ Bordeaux 2, UMR BFP1332, INRA, Villenave Dornon, France. RP Zhebentyayeva, TN (reprint author), Clemson Univ, Dept Biochem & Genet, Robert F Poole Agr Ctr, Room 154, Clemson, SC 29634 USA. EM tzhebe@clemson.edu; aabbott@bordeaux.inra.fr RI Bielenberg, Douglas/G-3893-2010; Fan, Shenghua/E-6400-2016 OI Bielenberg, Douglas/0000-0002-4853-2984; FU Robert and Lois Coker Trustees Chair in Molecular Genetics at the Department of Genetics and Biochemistry, Clemson University FX The study was carried out with financial support from the Robert and Lois Coker Trustees Chair in Molecular Genetics at the Department of Genetics and Biochemistry, Clemson University. We also are thankful to Kathy Brock for maintaining plants used in this study and Dr. Mike Wang at the David H. Murdock Research Institution (Kannapolis, NC) for guidance with Illumina Sequencing applications. NR 83 TC 12 Z9 13 U1 5 U2 59 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD FEB PY 2014 VL 10 IS 1 BP 35 EP 51 DI 10.1007/s11295-013-0660-6 PG 17 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA 291EA UT WOS:000329809300004 ER PT J AU Skoracka, A Kuczynski, L Rector, B Amrine, JW AF Skoracka, Anna Kuczynski, Lechoslaw Rector, Brian Amrine, James W., Jr. TI Wheat curl mite and dry bulb mite: untangling a taxonomic conundrum through a multidisciplinary approach SO BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY LA English DT Article DE 28S rDNA D2; Aceria tosichella; Aceria tulipae; cryptic species; DNA barcoding; host specificity; morphological variability; mtDNA COI; species complex; taxonomy ID ACERIA-TOSICHELLA KEIFER; DNA BARCODE DATA; ACARI ERIOPHYOIDEA; CRYPTIC DIVERSITY; NORTH-AMERICAN; TULIPAE KEIFER; SPECIES DELINEATION; MAXIMUM-LIKELIHOOD; MORPHOLOGY; LINEAGES AB Two economically important eriophyoid mites, Aceria tosichella (wheat curl mite; WCM) and Aceria tulipae (dry bulb mite; DBM), were frequently confounded in the world literature until the late 20th Century. Their morphological similarity and ambiguous data from plant-transfer and virus-transmission trials contributed to this confusion. Until recently, there was a general lack of knowledge about the existence of species complexes and it was not possible to accurately genotype tested mites. In the present study, two WCM genotypes of divergent host specificity (MT-1 and MT-2) and one DBM genotype were tested for the acceptance of Poaceae, Amarylidaceae, and Liliaceae species that were reported or suspected as hosts of WCM or DBM. The MT-1 lineage colonized all tested plants. Onion- and garlic-associated DBM populations did not colonize tulip and wild garlic, suggesting that host-acceptance variability exists within A.tulipae s.l. Morphometric analysis did not discriminate closely-related MT-1 and MT-2 genotypes but completely separated both WCM genotypes from DBM based on the larger overall body size of the latter. Three morphological traits combined to discriminate between the DBM and MT-1 genotypes, both of which can infest Amarylidaceae bulbs. In total, these combined DNA sequence, host-acceptance, morphometrical results unambiguously separated two WCM and one DBM genotypes. Similar studies on additional lineages of both WCM and DBM should ultimately dispel previous taxonomic confusion between these two species.(c) 2013 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 111, 421-436. C1 [Skoracka, Anna] Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Anim Taxon & Ecol, PL-61614 Poznan, Poland. [Kuczynski, Lechoslaw] Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Avian Biol & Ecol, PL-61614 Poznan, Poland. [Rector, Brian] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. [Amrine, James W., Jr.] W Virginia Univ, Morgantown, WV 26508 USA. RP Skoracka, A (reprint author), Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Anim Taxon & Ecol, Umultowska 89, PL-61614 Poznan, Poland. EM anna.skoracka@amu.edu.pl RI Kuczynski, Lechoslaw/E-5525-2011; Skoracka, Anna/E-6002-2011 OI Kuczynski, Lechoslaw/0000-0003-3498-5445; Skoracka, Anna/0000-0002-9485-532X FU Ministry of Science and Higher Education in Poland [NN 303089434] FX We thank Urszula Sobczynska and Wiktoria Szydlo (AMU) for help with the DNA sequencing, Danuta Banaszak and Ryszard Paliga for permission to collect plants, Philipp Chetverikov (Saint-Petersburg State University), and three anonymous reviewers for their corrections and suggestions that improved the manuscript. The study was funded by the Ministry of Science and Higher Education in Poland (grant no. NN 303089434). The authors declare that there are no conflicts of interest. AS and LK conceived and designed the study and analyzed the data. AS collected molecular and morphological data and performed the experiments. AS and BR wrote the manuscript. LK contributed to writing the methods and results, JWA contributed to writing the introduction and discussion. All authors read and approved the final manuscript. NR 71 TC 16 Z9 16 U1 2 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0024-4066 EI 1095-8312 J9 BIOL J LINN SOC JI Biol. J. Linnean Soc. PD FEB PY 2014 VL 111 IS 2 BP 421 EP 436 DI 10.1111/bij.12213 PG 16 WC Evolutionary Biology SC Evolutionary Biology GA 290FE UT WOS:000329738700015 ER PT J AU Jetter, A Egli, A Dawson-Hughes, B Staehelin, HB Stoecklin, E Goessl, R Henschkowski, J Bischoff-Ferrari, HA AF Jetter, Alexander Egli, Andreas Dawson-Hughes, Bess Staehelin, Hannes B. Stoecklin, Elisabeth Goessl, Richard Henschkowski, Jana Bischoff-Ferrari, Heike A. TI Pharmacokinetics of oral vitamin D-3 and calcifediol SO BONE LA English DT Article DE 25-hydroxyvitamin D-3; Pharmacokinetics; Plasma concentrations; Supplementation; Vitamin D-3; Calcifediol ID TANDEM MASS-SPECTROMETRY; INTESTINAL-ABSORPTION; MINERAL METABOLISM; D SUPPLEMENTATION; D DEFICIENCY; BONE LOSS; CALCIUM; 25-HYDROXYCHOLECALCIFEROL; WOMEN; 25-HYDROXYVITAMIN-D AB Aim: Long-term pharmacokinetics after supplementation with vitamin D-3 or calcifediol (the 25-hydroxyvitamin D-3 metabolite) is not well studied. Additionally, it is unclear whether bolus doses of vitamin D-3 or calcifediol lead to 25(OH)D-3 plasma concentrations considered desirable for fracture prevention (30 ng/mL). We therefore investigated plasma pharmacoldnetics of 25(OH)D-3 during different vitamin D-3 and calcifediol supplementation regimens. Methods: In this seven-arm, randomized, double-blind, controlled parallel-group study, 35 healthy females aged 50-70 years (5 per group) received 20 mu g calcifediol or vitaminD(3) daily, 140 mu g calcifediol or vitaminD(3) weekly, for 15 weeks, or a single bolus of either 140 mu g calcifediol, or vitaminD(3), or both. 25(OH)D-3 plasma concentrations were quantified using LC-MS/MS in 14 clinical visits among all participants. Results: For daily (weekly) dosing, the area under the concentration-time curve (AUC(0-24h)), which is the measure for exposure, was 28% (67%) higher after the first dose of calcifediol than after the first dose of vitamin D-3. After 15 weeks, this difference was 123% (178%). All women in the daily and weekly calcifediol groups achieved 25(OH)D-3 concentrations >30 ng/mL (mean, 16.8 days), but only 70% in the vitamin D-3 daily or weekly groups reached this concentration (mean, 68.4 days). A single dose of 140 mu g calcifediol led to 117% higher 25(OH)D-3 AUC(0-96h) values than 140 mu g vitamin D-3, while the simultaneous intake of both did not further increase exposure. Conclusions: Calcifediol given daily, weekly, or as a single bolus is about 2-3 times more potent in increasing plasma 25(OH)D-3 concentrations than vitamin D-3. Plasma 25(OH)D-3 concentrations of 30 ng/mL were reached more rapidly and reliably with calcifediol. (C) 2013 Elsevier Inc. All rights reserved. C1 [Jetter, Alexander] Univ Zurich Hosp, Dept Clin Pharmacol & Toxicol, CH-8091 Zurich, Switzerland. [Egli, Andreas; Henschkowski, Jana; Bischoff-Ferrari, Heike A.] Univ Zurich, Ctr Aging & Mobil, Zurich, Switzerland. [Egli, Andreas; Henschkowski, Jana] City Hosp Waid, Ctr Aging & Mobil, Zurich, Switzerland. [Dawson-Hughes, Bess] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Staehelin, Hannes B.] Univ Basel, Dept Geriatr, Basel, Switzerland. [Stoecklin, Elisabeth] DSM Nutr Prod Ltd, Basel, Switzerland. RP Bischoff-Ferrari, HA (reprint author), Univ Zurich Hosp, Dept Geriatr, Gloriastr 25, CH-8091 Zurich, Switzerland. EM Heike.Bischoff@usz.ch FU DSM Nutritional Products Research Development; Swiss National Science Foundation [PP00B-114864] FX This trial was funded by an independent investigator initiated grant provided by DSM Nutritional Products Research & Development and a Swiss National Science Foundation Professorship Grant (PP00B-114864, Bischoff-Ferrari HA). NR 26 TC 17 Z9 19 U1 1 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 8756-3282 EI 1873-2763 J9 BONE JI Bone PD FEB PY 2014 VL 59 BP 14 EP 19 DI 10.1016/j.bone.2013.10.014 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 287QZ UT WOS:000329558600003 PM 24516879 ER PT J AU Chao, K Kim, MS Chan, DE AF Chao, Kuanglin Kim, Moon S. Chan, Diane E. TI Control interface and tracking control system for automated poultry inspection SO COMPUTER STANDARDS & INTERFACES LA English DT Article DE Food safety; Automated poultry inspection; Tracking control; Spectroscopy ID 2-DIMENSIONAL CORRELATION SPECTROSCOPY; INFRARED REFLECTANCE; CHICKEN MEATS; CARCASSES; STORAGE AB A new Vis/NIR inspection system interface was developed to test and implement an automated chicken inspection system for online operation on commercial chicken processing lines. The spectroscopic system demonstrated effective spectral acquisition and data processing for real-time classification of chickens on a 140 bpm processing line. Real-time online testing successfully differentiated between wholesome and unwholesome birds using a neural network classification model with 20 input nodes that correctly classified 94% and 92% of wholesome and unwholesome birds, respectively. This work demonstrates the effectiveness of the Vis/NIR inspection system for accurate real-time product and shackle tracking on commercial chicken processing lines. (C) 2011 Published by Elsevier B.V. C1 [Chao, Kuanglin; Kim, Moon S.; Chan, Diane E.] USDA ARS, Environm Microbial & Food Safety Lab, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Chao, K (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, BARC East, Bldg 303,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM kevin.chao@ars.usda.giov NR 11 TC 2 Z9 2 U1 1 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-5489 EI 1872-7018 J9 COMPUT STAND INTER JI Comput. Stand. Interfaces PD FEB PY 2014 VL 36 IS 2 BP 271 EP 277 DI 10.1016/j.csi.2011.03.006 PG 7 WC Computer Science, Hardware & Architecture; Computer Science, Software Engineering SC Computer Science GA 289LC UT WOS:000329683000002 ER PT J AU Evans, DM Schoenholtz, SH Wigington, PJ Griffith, SM Floyd, WC AF Evans, Daniel M. Schoenholtz, Stephen H. Wigington, Parker J., Jr. Griffith, Stephen M. Floyd, William C. TI Spatial and temporal patterns of dissolved nitrogen and phosphorus in surface waters of a multi-land use basin SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Surface waters; Dissolved nutrients; Land management; Dissolved nitrogen; Phosphorus; Multi-land use; Landscape scale; Rivers; Streams ID CONTRASTING CLIMATIC YEARS; WESTERN OREGON; ORGANIC NITROGEN; SOURCE FERTILIZATION; ITALIAN RYEGRASS; STREAM; DYNAMICS; FOREST; EUTROPHICATION; LANDSCAPE AB Research on relationships between dissolved nutrients and land-use at the watershed scale is a high priority for protecting surface water quality. We measured dissolved nitrogen (DN) and ortho-phosphorus (P) along 130 km of the Calapooia River (Oregon, USA) and 44 of its sub-basins for 3 years to test for associations with land-use. Nutrient concentrations were analyzed for spatial and seasonal patterns and for relationships with land-use and stream discharge. Ortho-P and DN were higher in lower-elevation sub-basins dominated by poorly drained soils and agricultural production compared with higher-elevation sub-basins dominated by well-drained soils and forests. Eight lower basins had at least one sample period with nitrate-N > 10 mg L-1. The Calapooia River had lower concentrations of dissolved nutrients compared with lower sub-basins, often by an order of magnitude. Dissolved organic N represented a greater proportion of DN in the upper forested sub-basins. Seasonal nutrient concentrations had strong positive correlations to the percent of a sub-basin that was managed for agriculture in all seasons (p values a parts per thousand currency signaEuro parts per thousand 0.019) except summer. Results suggest that agricultural lands are contributing to stream nutrient concentrations. However, poorly drained soils in agricultural areas may also contribute to the strong relationships that we found between dissolved nutrients and agriculture. C1 [Evans, Daniel M.] Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Schoenholtz, Stephen H.] Virginia Tech, Virginia Water Resources Res Ctr, Blacksburg, VA 24061 USA. [Wigington, Parker J., Jr.] US EPA, Corvallis, OR 97333 USA. [Griffith, Stephen M.] USDA ARS, Corvallis, OR 97330 USA. [Floyd, William C.] Minist Forests Lands & Nat Resource Operat, Nanaimo, BC V9T 6E9, Canada. RP Evans, DM (reprint author), Virginia Tech, Dept Crop & Soil Environm Sci, 330 Smyth Hall, Blacksburg, VA 24061 USA. EM daniel76@vt.edu; stephen.schoenholtz@vt.edu; wigington.jim@epa.gov; Steve.Griffith@ars.usda.gov; william.floyd@gov.bc.ca FU Forest Research Laboratory Fish and Wildlife Habitat in Managed Forests Research Program at Oregon State University; USDA Agricultural Research Service FX This research was supported by the Forest Research Laboratory Fish and Wildlife Habitat in Managed Forests Research Program at Oregon State University and the USDA Agricultural Research Service. In-kind support was provided by the US Environmental Protection Agency through the time of one of the co-authors. This manuscript has been subjected to agency review and approved for publication. We thank Machelle Nelson and Donald Streeter for providing lab and field support. The use of trade, firm, or corporation names in this manuscript is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the USDA Agricultural Research Service or the U.S. Environmental Protection Agency of any product or service to the exclusion of others that may be suitable. NR 46 TC 9 Z9 10 U1 5 U2 70 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD FEB PY 2014 VL 186 IS 2 BP 873 EP 887 DI 10.1007/s10661-013-3428-4 PG 15 WC Environmental Sciences SC Environmental Sciences & Ecology GA 288YE UT WOS:000329647300018 PM 24046242 ER PT J AU Azuma, DL Gray, A AF Azuma, David L. Gray, Andrew TI Effects of changing forest land definitions on forest inventory on the West Coast, USA SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Forest definition; Forest inventory; Relative stocking; Canopy cover ID COVER AB A key function of forest inventory is to detect changes in the area of forest land over time, yet different definitions of forest land are used in different regions of the world. Changes in the definition of forest intended to improve international consistency can affect the ability to quantify true changes over time. The objective of this study was to evaluate the effects of a definitional change from relative stocking to canopy cover on the area classified as forest land and its relationship to species and forest density in California, Oregon, and Washington. Both western Juniper and ponderosa pine will yield higher estimates of forest land area using a canopy cover definition in comparison to a stocking-based definition, with the difference being most pronounced where land is marginally forested. The change in definition may result in an additional 146,000 ha of forest land identified on the West Coast. Measuring marginal forest lands with both metrics for the first cycle after implementation should make it possible to distinguish real change from definitional change. C1 [Azuma, David L.] US Forest Serv, USDA, Pacific Northwest Stn,Portland Forestry Sci Lab, Forest Inventory & Anal Program, Portland, OR 97205 USA. [Gray, Andrew] US Forest Serv, USDA, Pacific NW Res Stn,Corvallis Forestry Sci Lab, Forest Inventory & Anal Program, Corvallis, OR 97331 USA. RP Azuma, DL (reprint author), US Forest Serv, USDA, Pacific Northwest Stn,Portland Forestry Sci Lab, Forest Inventory & Anal Program, 620 SW Main St,Suite 400, Portland, OR 97205 USA. EM dazuma@fs.fed.us; agray01@fs.fed.us NR 23 TC 0 Z9 0 U1 0 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD FEB PY 2014 VL 186 IS 2 BP 1001 EP 1007 DI 10.1007/s10661-013-3434-6 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA 288YE UT WOS:000329647300029 PM 24072525 ER PT J AU Sigua, GC Chase, CC AF Sigua, Gilbert C. Chase, Chad C., Jr. TI Groundwater phosphorus in forage-based landscape with cow-calf operation SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Groundwater; Phosphorus; Beef cattle; Landscape; Bahiagrass; Erosion; MCL ID WATER-QUALITY; SOIL-PHOSPHORUS; PASTURE; NITROGEN; MANAGEMENT; RUNOFF; LAND; TRANSPORT; SURFACE; FLORIDA AB Forage-based cow-calf operations may have detrimental impacts on the chemical status of groundwater and streams and consequently on the ecological and environmental status of surrounding ecosystems. Assessing and controlling phosphorus (P) inputs are, thus, considered the key to reducing eutrophication and managing ecological integrity. In this paper, we monitored and evaluated P concentrations of groundwater (GW) compared to the concentration of surface water (SW) P in forage-based landscape with managed cow-calf operations for 3 years (2007-2009). Groundwater samples were collected from three landscape locations along the slope gradient (GW1 10-30 % slope, GW2 5-10 % slope, and GW3 0-5 % slope). Surface water samples were collected from the seepage area (SW 0 % slope) located at the bottom of the landscape. Of the total P collected (averaged across year) in the landscape, 62.64 % was observed from the seepage area or SW compared with 37.36 % from GW (GW1 = 8.01 %; GW2 = 10.92 %; GW3 = 18.43 %). Phosphorus in GW ranged from 0.02 to 0.20 mg L-1 while P concentration in SW ranged from 0.25 to 0.71 mg L-1. The 3-year average of P in GW of 0.09 mg L-1 was lower than the recommended goal or the Florida's numeric nutrients standards (NNS) of 0.12 mg P L-1. The 3-year average of P concentration in SW of 0.45 mg L-1 was about fourfold higher than the Florida's NNS value. Results suggest that cow-calf operation in pasture-based landscape would contribute more P to SW than in the GW. The risk of GW contamination by P from animal agriculture production system is limited, while the solid forms of P subject to loss via soil erosion could be the major water quality risk from P. C1 [Sigua, Gilbert C.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Chase, Chad C., Jr.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. RP Sigua, GC (reprint author), ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. EM gilbert.sigua@ars.usda.gov NR 55 TC 1 Z9 1 U1 2 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD FEB PY 2014 VL 186 IS 2 BP 1317 EP 1326 DI 10.1007/s10661-013-3482-y PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA 288YE UT WOS:000329647300055 PM 24150714 ER PT J AU Kopittke, PM Menzies, NW Wang, P McKenna, BA Wehr, JB Lombi, E Kinraide, TB Blamey, FPC AF Kopittke, Peter M. Menzies, Neal W. Wang, Peng McKenna, Brigid A. Wehr, J. Bernhard Lombi, Enzo Kinraide, Thomas B. Blamey, F. Pax C. TI THE RHIZOTOXICITY OF METAL CATIONS IS RELATED TO THEIR STRENGTH OF BINDING TO HARD LIGANDS SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Binding; Mechanism of toxicity; Metal; Root growth; Symptom ID ROOT ELONGATION; CELL-WALL; ALUMINUM TOXICITY; TRANSITION ZONE; HYDRATED ROOTS; DISTAL PART; ZEA-MAYS; ION; COWPEA; GROWTH AB Mechanisms whereby metal cations are toxic to plant roots remain largely unknown. Aluminum, for example, has been recognized as rhizotoxic for approximately 100 yr, but there is no consensus on its mode of action. The authors contend that the primary mechanism of rhizotoxicity of many metal cations is nonspecific and that the magnitude of toxic effects is positively related to the strength with which they bind to hard ligands, especially carboxylate ligands of the cell-wall pectic matrix. Specifically, the authors propose that metal cations have a common toxic mechanism through inhibiting the controlled relaxation of the cell wall as required for elongation. Metal cations such as Al3+ and Hg2+, which bind strongly to hard ligands, are toxic at relatively low concentrations because they bind strongly to the walls of cells in the rhizodermis and outer cortex of the root elongation zone with little movement into the inner tissues. In contrast, metal cations such as Ca2+, Na+, Mn2+, and Zn2+, which bind weakly to hard ligands, bind only weakly to the cell wall and move farther into the root cylinder. Only at high concentrations is their weak binding sufficient to inhibit the relaxation of the cell wall. Finally, different mechanisms would explain why certain metal cations (for example, Tl+, Ag+, Cs+, and Cu2+) are sometimes more toxic than expected through binding to hard ligands. The data presented in the present study demonstrate the importance of strength of binding to hard ligands in influencing a range of important physiological processes within roots through nonspecific mechanisms. (c) 2013 SETAC C1 [Kopittke, Peter M.; Menzies, Neal W.; Wang, Peng; McKenna, Brigid A.; Wehr, J. Bernhard; Blamey, F. Pax C.] Univ Queensland, Sch Agr & Food Sci, St Lucia, Qld, Australia. [Lombi, Enzo] Univ S Australia, Ctr Environm Risk Assessment & Remediat, Mawson Lakes, SA, Australia. [Kinraide, Thomas B.] ARS, USDA, Sharon, MA USA. RP Kopittke, PM (reprint author), Univ Queensland, Sch Agr & Food Sci, St Lucia, Qld, Australia. EM p.kopittke@uq.edu.au RI Kopittke, Peter/A-6026-2011; Wang, Peng/E-2008-2012; Menzies, Neal/L-1338-2013; Lombi, Enzo/F-3860-2013 OI Kopittke, Peter/0000-0003-4948-1880; Wang, Peng/0000-0001-8622-8767; Menzies, Neal/0000-0003-0207-070X; Lombi, Enzo/0000-0003-3384-0375 FU Australian Research Council (ARC) [FT120100277]; ARC [DE130100943, FT100100337] FX P. Kopittke is the recipient of an Australian Research Council (ARC) Future Fellowship (FT120100277), P. Wang is the recipient of an ARC Discovery Early Career Researcher Award (DE130100943), and E. Lombi is the recipient of an ARC Future Fellowship (FT100100337). NR 78 TC 12 Z9 12 U1 1 U2 39 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD FEB PY 2014 VL 33 IS 2 BP 268 EP 277 DI 10.1002/etc.2435 PG 10 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA 287QF UT WOS:000329556600002 PM 24142597 ER PT J AU Juneja, V Mukhopadhyay, S Marks, H Mohr, TB Warning, A Datta, A AF Juneja, Vijay Mukhopadhyay, Sudarsan Marks, Harry Mohr, Tim B. Warning, Alex Datta, Ashim TI Predictive Thermal Inactivation Model for Effects and Interactions of Temperature, NaCl, Sodium Pyrophosphate, and Sodium Lactate on Listeria monocytogenes in Ground Beef SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Listeria monocytogenes; NaCl; Sodium pyrophosphate; Sodium lactate; Thermal inactivation; Predictive model ID COOKING AB The effects and interactions of heating temperature (60 A degrees C to 73.9 A degrees C), salt (0.0 % to 4.5 % w/v), sodium pyrophosphate (0.0 % to 0.5 % w/v), and sodium lactate (0.0 % to 4.5 % w/v) on the heat resistance of a five-strain mixture of Listeria monocytogenes in 75 % lean ground beef were examined. Meat samples in sterile filtered stomacher bags were heated in a temperature controlled waterbath to determine thermal death times. The recovery medium was tryptic soy agar supplemented with 0.6 % yeast extract and 1 % sodium pyruvate. Weibull survival functions were employed to model the primary survival curves. Then, survival curve-specific estimated parameter values obtained from the Weibull model were used for determining a secondary model. The results indicate that temperature and salt have a large impact on the inactivation kinetics of L. monocytogenes, while sodium lactate (NaL) has an impact in the presence of salt. The model presented in this paper for predicting inactivation of L. monocytogenes can be used as an aid in designing lethality treatments meant to control the presence of this pathogen in ready-to-eat products. C1 [Juneja, Vijay; Mukhopadhyay, Sudarsan] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Marks, Harry] Food Safety Inspect Serv, USDA, Washington, DC 20250 USA. [Mohr, Tim B.] US Food Safety & Inspect Serv, Div Microbiol, Off Publ Hlth Sci, USDA, Salem, OR 97301 USA. [Warning, Alex; Datta, Ashim] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY 14853 USA. RP Juneja, V (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Vijay.Juneja@ars.usda.gov NR 8 TC 4 Z9 5 U1 5 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD FEB PY 2014 VL 7 IS 2 BP 437 EP 446 DI 10.1007/s11947-013-1102-z PG 10 WC Food Science & Technology SC Food Science & Technology GA 288WF UT WOS:000329642000010 ER PT J AU Shao, DY Atungulu, GG Pan, ZL Yue, TL Zhang, A Fan, ZL AF Shao, Dongyan Atungulu, Griffiths G. Pan, Zhongli Yue, Tianli Zhang, Ang Fan, Zhiliang TI Characteristics of Isolation and Functionality of Protein from Tomato Pomace Produced with Different Industrial Processing Methods SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Tomato seed meals; Protein isolation; Functional properties; Industrial tomato-processing methods; Tomato pomace ID BIOLOGICAL EVALUATION; SEED MEALS; FLOURS; L.; RAW; CONCENTRATE; OPTIMIZATION; WASTE; NUT AB The seeds separated from tomato pomace may contain valuable protein with unique functional properties. The objectives of this research were to study the impact of industrial hot and cold break tomato processes on protein isolation from defatted tomato seed meal and determine the protein-related functional properties of defatted and non-defatted seed meals. The results showed that the high temperature of hot break process denatured the protein, resulting in the lower protein extraction yield from 9.07 % to 26.29 % for defatted hot break tomato seed (DHTS) compared to from 25.60 % to 32.56 % for defatted cold break tomato seed (DCTS) under various extraction conditions. Hot break process also significantly influenced protein-related functional properties of seed meals. Compared to DCTS, DHTS had higher water absorption capacity (WAC) and oil absorption capacity (OAC) based on the protein weight in the seed meal, but lower emulsifying ability (EA), emulsifying stability (ES), foaming capacity (FC), and foaming stability (FS) based on the whole seed sample weight. When compared to commercial soybean protein isolate (SP), the meals of hot break tomato seed (HTS), DHTS, and DCTS showed higher bulk density and WAC values. The FC and FS of tomato meals were inferior while the ES was superior to SP. High alkaline pH was beneficial to the protein extraction and achieved better EA, ES, FC and FS of all the samples. The results indicated that tomato seed meals have a great potential to be used as functional food ingredients. C1 [Shao, Dongyan; Yue, Tianli] Northwest Agr & Forestry Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China. [Shao, Dongyan; Atungulu, Griffiths G.; Pan, Zhongli; Zhang, Ang; Fan, Zhiliang] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Pan, Zhongli] ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Zhang, Ang] Northwest Agr & Forestry Univ, Coll Enol, Yangling 712100, Shaanxi, Peoples R China. RP Pan, ZL (reprint author), ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM zhongli.pan@ars.usda.gov NR 46 TC 4 Z9 6 U1 3 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD FEB PY 2014 VL 7 IS 2 BP 532 EP 541 DI 10.1007/s11947-013-1057-0 PG 10 WC Food Science & Technology SC Food Science & Technology GA 288WF UT WOS:000329642000020 ER PT J AU Knight, AL Light, DM AF Knight, A. L. Light, D. M. TI Combined approaches using sex pheromone and pear ester for behavioural disruption of codling moth (Lepidoptera: Tortricidae) SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE apple; Cydia pomonella; lures; mating disruption; monitoring ID MATING DISRUPTION; CYDIA-POMONELLA; APPLE ORCHARDS; DISPENSERS; CODLEMONE; DISPERSAL; EFFICACY; LARVAE AB Attractive properties of pear ester, ethyl (E,Z)-2,4-decadienoate, and codlemone, (E,E)-8,10-dodecadien-1-ol, the sex pheromone of codling moth, Cydia pomonella (L.), were utilized in experiments on behavioural disruption of mating. Standard dispensers loaded with codlemone alone or in combination with pear ester (combo) were applied at 500-1000/ha. Larger (10-fold) combo dispensers (Meso) were evaluated at a rate of 80/ha. The addition of microencapsulated pear ester, PE-MEC, sprayed with insecticides at 30ml/ha was also evaluated. Male moth catches in unmated female-baited traps were lower in standard combo dispenser than in codlemone dispenser-treated plots. Female moth catch in traps baited with the combination of pear ester, codlemone and acetic acid was lower in standard combo dispenser than in codlemone dispenser-treated plots. In 12 comparative experiments spanning from 2006 to 2012, male moth catch in unmated female-baited traps was consistently and significantly lower in combo than in codlemone dispenser-treated plots. Male catch in codlemone-baited traps did not differ between dispenser treatments in eight studies from 2006 to 2009. These results emphasize the benefit of alternatively using traps baited with unmated females over codlemone lures for the analysis of dispenser activity. Fruit injury was significantly reduced with the addition of PE-MEC to insecticide applications across untreated and dispenser treatments. Proportion of unmated females trapped was higher in standard combo dispenser than in codlemone dispenser-treated and untreated plots. Similarly, the proportion of unmated females caught was higher in the Meso combo dispenser than in nearby or distant codlemone dispenser-treated plots. These field studies conducted in apple over 3years demonstrate that adding pear ester both to pheromone dispensers, either standard or Meso, and to supplementary insecticide sprays can provide a significant increase in the disruption of sexual communication, reductions in female mating and reductions in fruit injury. C1 [Knight, A. L.] ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. [Light, D. M.] ARS, USDA, Albany, CA USA. RP Knight, AL (reprint author), ARS, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM alan.knight@ars.usda.gov NR 39 TC 5 Z9 5 U1 2 U2 37 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD FEB PY 2014 VL 138 IS 1-2 BP 96 EP 108 DI 10.1111/jen.12071 PG 13 WC Entomology SC Entomology GA 287GN UT WOS:000329530100013 ER PT J AU Rolla, AAD Carvalho, JDC Fuganti-Pagliarini, R Engels, C do Rio, A Marin, SRR de Oliveira, MCN Beneventi, MA Marcelino-Guimaraes, FC Farias, JRB Neumaier, N Nakashima, K Yamaguchi-Shinozaki, K Nepomuceno, AL AF de Paiva Rolla, Amanda Alves Correa Carvalho, Josirley de Fatima Fuganti-Pagliarini, Renata Engels, Cibelle do Rio, Alexandre Rockenbach Marin, Silvana Regina Neves de Oliveira, Maria Cristina Beneventi, Magda A. Marcelino-Guimaraes, Francismar Correa Boucas Farias, Jose Renato Neumaier, Norman Nakashima, Kazuo Yamaguchi-Shinozaki, Kazuko Nepomuceno, Alexandre Lima TI Phenotyping soybean plants transformed with rd29A:AtDREB1A for drought tolerance in the greenhouse and field SO TRANSGENIC RESEARCH LA English DT Article DE BR 16; DREB1A; Embrapa 48; Transpiration; Transcription factor; Water deficit ID RESPONSIVE GENE-EXPRESSION; TRANSCRIPTION FACTOR; STRESS TOLERANCE; TRANSGENIC WHEAT; WATER-STRESS; DREB1A GENE; HIGH-SALT; ARABIDOPSIS; RESISTANCE; RICE AB The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of several genes related to abiotic stress defense responses. This work assessed the performance of soybean plants overexpressing the TF DREB1A under drought conditions in the field and in the greenhouse. Drought was simulated in the greenhouse by progressively drying the soil of pot cultures of the P58 and P1142 lines. In the field, the performance of the P58 line and of 09D-0077, a cross between the cultivars BR16 and P58, was evaluated under four different water regimes: irrigation, natural drought (no irrigation) and water stress created using rain-out shelters in the vegetative or reproductive stages. Although the dehydration-responsive element-binding protein (DREB) plants did not outperform the cultivar BR16 in terms of yield, some yield components were increased when drought was introduced during the vegetative stage, such as the number of seeds, the number of pods with seeds and the total number of pods. The greenhouse data suggest that the higher survival rates of DREB plants are because of lower water use due to lower transpiration rates under well watered conditions. Further studies are needed to better characterize the soil and atmospheric conditions under which these plants may outperform the non-transformed parental plants. C1 [de Paiva Rolla, Amanda Alves; Engels, Cibelle] Univ Estadual Londrina, Dept Gen Biol, BR-86051990 Londrina, Brazil. [Correa Carvalho, Josirley de Fatima; Fuganti-Pagliarini, Renata; do Rio, Alexandre; Rockenbach Marin, Silvana Regina; Neves de Oliveira, Maria Cristina; Marcelino-Guimaraes, Francismar Correa; Boucas Farias, Jose Renato; Neumaier, Norman; Nepomuceno, Alexandre Lima] EMBRAPA Soybean, BR-86001970 Londrina, Brazil. [Beneventi, Magda A.] Univ Fed Rio Grande do Sul, BR-90040060 Porto Alegre, RS, Brazil. [Nakashima, Kazuo] Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3058686, Japan. [Yamaguchi-Shinozaki, Kazuko] Univ Tokyo, Lab Plant Mol Physiol, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan. [Nepomuceno, Alexandre Lima] ARS, USDA, Ctr Plant Gene Express, Albany, CA 94710 USA. RP Nepomuceno, AL (reprint author), EMBRAPA Soybean, Rodovia Carlos Joao Strass,POB 231, BR-86001970 Londrina, Brazil. EM amandaaapr@gmail.com; josie.carvalho@hotmail.co.uk; renatafuganti@gmail.com; ciengels@gmail.com; arioagro@hotmail.com; silvana@cnpso.embrapa.br; maria.neves-oliveira@embrapa.br; mabeneventi@gmail.com; francismar.marcelino@embrapa.br; joserenato.farias@embrapa.br; norman.neumaier@embrapa.br; kazuo.nakashima@affrc.go.jp; akys@mail.ecc.u-tokyo.ac.jp; alexlimanepo@gmail.com RI Neumaier, Norman/I-9545-2014; Yamaguchi-Shinozaki, Kazuko/K-9004-2015; OI Fuganti-Pagliarini, Renata /0000-0001-9282-2826 FU Science and Technology Research Partnership for Sustainable Development (SATREPS) FX This work was supported by the Science and Technology Research Partnership for Sustainable Development (SATREPS) of the Japan Science and Technology Agency/Japan International Cooperation Agency, JIRCAS, Empresa Brasileira de Pesquisa Agropecuaria, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Londrina State University (UEL)/Brazil. NR 35 TC 16 Z9 16 U1 2 U2 53 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD FEB PY 2014 VL 23 IS 1 BP 75 EP 87 DI 10.1007/s11248-013-9723-6 PG 13 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA 288UG UT WOS:000329636800006 ER PT J AU McKenzie, D Shankar, U Keane, RE Stavros, EN Heilman, WE Fox, DG Riebau, AC AF McKenzie, Donald Shankar, Uma Keane, Robert E. Stavros, E. Natasha Heilman, Warren E. Fox, Douglas G. Riebau, Allen C. TI Smoke consequences of new wildfire regimes driven by climate change SO EARTHS FUTURE LA English DT Review DE climate change; smoke transport; feedbacks; coupled models ID GENERAL-CIRCULATION MODEL; TURBULENT KINETIC-ENERGY; GLOBAL VEGETATION MODEL; FIRE-SUCCESSION MODELS; WESTERN UNITED-STATES; EARLY-WARNING SIGNALS; CROSS-SCALE ANALYSIS; SUB-ALPINE FORESTS; LANDSCAPE-FIRE; AIR-POLLUTION AB Smoke from wildfires has adverse biological and social consequences, and various lines of evidence suggest that smoke from wildfires in the future may be more intense and widespread, demanding that methods be developed to address its effects on people, ecosystems, and the atmosphere. In this paper, we present the essential ingredients of a modeling system for projecting smoke consequences in a rapidly warming climate that is expected to change wildfire regimes significantly. We describe each component of the system, offer suggestions for the elements of a modeling agenda, and provide some general guidelines for making choices among potential components. We address a prospective audience of researchers whom we expect to be fluent already in building some or many of these components, so we neither prescribe nor advocate particular models or software. Instead, our intent is to highlight fruitful ways of thinking about the task as a whole and its components, while providing substantial, if not exhaustive, documentation from the primary literature as reference. This paper provides a guide to the complexities of smoke modeling under climate change, and a research agenda for developing a modeling system that is equal to the task while being feasible with current resources. C1 [McKenzie, Donald] US Forest Serv, Pacific Wildland Fire Sci Lab, Pacific Northwest Res Stn, Seattle, WA 98103 USA. [Shankar, Uma] Univ N Carolina, Inst Environm, Chapel Hill, NC USA. [Keane, Robert E.] US Forest Serv, Missoula Fire Sci Lab, Rocky Mt Res Stn, Missoula, MT USA. [Stavros, E. Natasha] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Heilman, Warren E.] US Forest Serv, Northern Res Stn, E Lansing, MI USA. [Fox, Douglas G.] Colorado State Univ, Cooperat Inst Res Atmosphere, Ft Collins, CO USA. [Riebau, Allen C.] Nine Points South Tech Pty Ltd, Clarkson, WA, Australia. RP McKenzie, D (reprint author), US Forest Serv, Pacific Wildland Fire Sci Lab, Pacific Northwest Res Stn, Seattle, WA 98103 USA. EM dmck@uw.edu FU Joint Fire Science Program [12-S-01-2]; Pacific Northwest Research Station, US Forest Service FX Funding for this paper was provided by the Joint Fire Science Program under project 12-S-01-2, and by the Pacific Northwest Research Station, US Forest Service. R. Norheim assisted with cartography, and E. Eberhardt with graphics. J.H. Bowden and L. Ran provided helpful comments. NR 196 TC 4 Z9 4 U1 5 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2328-4277 J9 EARTHS FUTURE JI Earth Future PD FEB PY 2014 VL 2 IS 2 BP 35 EP 59 DI 10.1002/2013EF000180 PG 25 WC Environmental Sciences; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Geology; Meteorology & Atmospheric Sciences GA CN0TC UT WOS:000358125500002 ER PT J AU Baranowski, T AF Baranowski, Tom TI Lying (or Maybe Just Misleading) With (or Without) Statistics SO GAMES FOR HEALTH JOURNAL LA English DT Editorial Material ID P VALUES; PILOT; FEASIBILITY; TRIALS; DESIGN; POWER C1 Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Pediat Behav Nutr & Phys Act,Dept Pediat, Houston, TX 77030 USA. RP Baranowski, T (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Pediat Behav Nutr & Phys Act,Dept Pediat, Houston, TX 77030 USA. OI Baranowski, Tom/0000-0002-0653-2222 NR 14 TC 1 Z9 1 U1 2 U2 2 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD FEB PY 2014 VL 3 IS 1 BP 1 EP 2 DI 10.1089/g4h.2014.1713 PG 2 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA CU2VK UT WOS:000363381500001 PM 26197247 ER PT J AU Clay, DE Clay, SA Reitsma, KD Dunn, BH Smart, AJ Carlson, GG Horvath, D Stone, JJ AF Clay, David E. Clay, Sharon A. Reitsma, Kurtis D. Dunn, Barry H. Smart, Alexander J. Carlson, Gregg G. Horvath, David Stone, James J. TI Does the conversion of grasslands to row crop production in semi-arid areas threaten global food supplies? SO GLOBAL FOOD SECURITY-AGRICULTURE POLICY ECONOMICS AND ENVIRONMENT LA English DT Article DE Precision farming; Soil health; Cropping systems; Extreme climatic events AB In the world's semi-arid regions, high crop demands have produced short term economic incentives to convert food production on native grasslands to dryland row crop food production, while genetic enhancements and equipment have reduced the risk of crop failure. The objectives of this paper were to discuss (1) the importance of considering the long-term sustainability of changing land use in semi-arid regions; (2) the impact of extreme climatic events on ecosystem functioning; and (3) factors contributing to higher crop yields in semi-arid regions. Semi-arid regions contain fragile areas where extreme climate events may be a tipping point that converts an apparent sustainable system to a non-sustainable ecosystem. However, semi-arid regions also contain zones where "better" management practices have reduced the agricultural impacts on the environment, increased soil carbon levels, and stimulated economic development. Research suggests that food production can be increased by enhancing the productivity of existing cropped land. However, this statement does not infer that crop production on all existing cropped lands in semi-arid regions is sustainable. Worldwide, targeted research should be conducted to clearly identify local barriers to conservation practice adoption and identify the long-term ramifications of extreme climatic events and land-use changes on semi-arid ecosystem functioning. (C) 2014 The Authors. Published by Elsevier B.V.Open access under CC BY-NC-ND license. C1 [Clay, David E.] S Dakota State Univ, Dept Plant Sci, Bookings, SD 57007 USA. [Clay, Sharon A.; Reitsma, Kurtis D.; Dunn, Barry H.; Smart, Alexander J.; Carlson, Gregg G.] S Dakota State Univ, Bookings, SD 57007 USA. [Horvath, David] ARS, USDA, Fargo, ND 58102 USA. [Stone, James J.] South Dakota Sch Mines & Technol, Rapid City, SD 57701 USA. RP Clay, DE (reprint author), S Dakota State Univ, Dept Plant Sci, Bookings, SD 57007 USA. EM david.clay@sdstate.edu OI Horvath, David/0000-0002-8458-7691 FU South Dakota State University, South Dakota; USDA-AFRI; USDA-NRCS-CIG FX Funding was provided by South Dakota State University, South Dakota, USDA-AFRI, and USDA-NRCS-CIG. NR 157 TC 6 Z9 6 U1 2 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2211-9124 J9 GLOB FOOD SECUR-AGR JI Glob. Food Secur.-Agric.Policy PD FEB PY 2014 VL 3 IS 1 BP 22 EP 30 DI 10.1016/j.gfs.2013.12.002 PG 9 WC Food Science & Technology SC Food Science & Technology GA V42EA UT WOS:000209595900004 ER PT J AU Becker, C Lord, S Studenski, S Warden, S Fielding, R Recknor, C Hochberg, M Ferrari, S Blain, H Binder, E Rolland, Y Hu, LJ Ahmad, Q Pacuch, K Gomez, E Benichou, O AF Becker, Clemens Lord, Stephen Studenski, Stephanie Warden, Stuart Fielding, Roger Recknor, Christopher Hochberg, Marc Ferrari, Serge Blain, Hubert Binder, Ellen Rolland, Yves Hu, Leijun Ahmad, Qasim Pacuch, Kelli Gomez, Elisa Benichou, Olivier TI Phase 2 Randomized, Double Blind, Placebo Controlled Trial of Anti-Myostatin Antibody LY2495655 in Older Fallers With Low Muscle Strength. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the American-Society-for-Bone-and-Mineral-Research CY SEP 12-15, 2014 CL Houston, TX SP Amer Soc Bone & Mineral Res C1 [Becker, Clemens] Robert Bosch Krankenhaus, Stuttgart, Germany. [Lord, Stephen] UNSW, NeuRA, Sydney, NSW, Australia. [Studenski, Stephanie] Univ Pittsburgh, Pittsburgh, PA 15260 USA. [Warden, Stuart] Indiana Univ, Sch Hlth & Rehabil Sci, Bloomington, IN 47405 USA. [Fielding, Roger] Tufts Univ, Jean Mayer USDA HNRCA, Medford, MA USA. [Recknor, Christopher] United Osteoporosis Ctr, Gainesville, FL USA. [Hochberg, Marc] Univ Maryland, Sch Med, College Pk, MD USA. [Ferrari, Serge] Univ Hosp Geneva, Geneva, Switzerland. [Blain, Hubert] Univ Montpellier I, Montpellier Univ Hosp, MacVia LR, F-34006 Montpellier, France. [Binder, Ellen] Washington Univ, Sch Med, St Louis, MO 63130 USA. [Rolland, Yves] CHU Toulouse, Gerontopole Toulouse, Toulouse, France. [Hu, Leijun; Ahmad, Qasim; Pacuch, Kelli; Gomez, Elisa] Eli Lilly & Co, Le Creusot, France. [Benichou, Olivier] Eli Lilly & Co, Le Creusot, France. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD FEB PY 2014 VL 29 SU 1 MA 1011 BP S5 EP S5 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CK9ZS UT WOS:000356598700012 ER PT J AU Cao, J Picklo, M AF Cao, Jay Picklo, Matthew TI Differential effects of involuntary running on bone structure of high-fat diet-induced obese rats. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the American-Society-for-Bone-and-Mineral-Research CY SEP 12-15, 2014 CL Houston, TX SP Amer Soc Bone & Mineral Res C1 [Cao, Jay] USDA ARS, Washington, DC 20250 USA. [Picklo, Matthew] Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD FEB PY 2014 VL 29 SU 1 MA SU0158 BP S258 EP S258 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CK9ZS UT WOS:000356598701467 ER PT J AU Qin, WP Li, XD Cao, J Collier, L Peng, YZ Feng, J Li, JL Qin, YW Brown, T Ke, HZ Bauman, WA Cardozo, C AF Qin, Weiping Li, Xiaodong Cao, Jay Collier, Lauren Peng, Yuanzhen Feng, Jerry Li, Jiliang Qin, Yiwen Brown, Tom Ke, Hua Zhu (David) Bauman, William A. Cardozo, Christopher TI Mice with Sclerostin Gene Deficiency are Resistant to Bone Loss after Acute Spinal Cord Injury SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the American-Society-for-Bone-and-Mineral-Research CY SEP 12-15, 2014 CL Houston, TX SP Amer Soc Bone & Mineral Res C1 [Qin, Weiping; Collier, Lauren; Qin, Yiwen; Bauman, William A.; Cardozo, Christopher] James J Peters Va Med Ctr, Bronx, NY USA. [Li, Xiaodong; Brown, Tom; Ke, Hua Zhu (David)] Amgen Inc, Thousand Oaks, CA USA. [Cao, Jay] ARS, USDA, Lincoln, NE USA. [Peng, Yuanzhen] Mt Sinai Sch Med, New York, NY USA. [Feng, Jerry] Baylor Coll Dent, TX A&M, Dallas, TX 75246 USA. [Li, Jiliang] Indiana Univ Purdue Univ Indianapolis, Indianapolis, IN USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD FEB PY 2014 VL 29 SU 1 MA MO0118 BP S385 EP S386 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CK9ZS UT WOS:000356598702271 ER PT J AU Sahni, S Wang, N Dufour, A Kiel, D Benjamin, E Murabito, J Vita, J Hannan, M Jacques, P McLean, R Fielding, R Ramachandran, V Mitchell, G Hamburg, N AF Sahni, Shivani Wang, Na Dufour, Alyssa Kiel, Douglas Benjamin, Emelia Murabito, Joanne Vita, Joseph Hannan, Marian Jacques, Paul McLean, Robert Fielding, Roger Ramachandran, Vasan Mitchell, Gary Hamburg, Naomi TI Increased peripheral vascular flow and aortic stiffness are associated with higher lean mass but lower muscle quality in middle-aged and older adults: the Framingham Heart Study. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the American-Society-for-Bone-and-Mineral-Research CY SEP 12-15, 2014 CL Houston, TX SP Amer Soc Bone & Mineral Res C1 [Sahni, Shivani] Hebrew SeniorLife, Inst Aging Res, Roslindale, MA USA. [Sahni, Shivani; Hannan, Marian; McLean, Robert] Harvard Univ, Sch Med, Cambridge, MA 02138 USA. [Wang, Na] Boston Univ, Sch Publ Hlth, Boston, MA 02215 USA. [Dufour, Alyssa; Kiel, Douglas] Hebrew SeniorLife, Roslindale, MA USA. [Benjamin, Emelia] Boston Univ, Sch Publ Hlth, Framingham Heart Study, Sch Med, Boston, MA 02215 USA. [Murabito, Joanne] Framingham Heart Dis Epidemiol Study, Framingham, MA USA. [Murabito, Joanne; Vita, Joseph; Hamburg, Naomi] Boston Univ, Sch Med, Boston, MA 02215 USA. [Hannan, Marian] HSL Inst Aging Res, Boston, MA USA. [Jacques, Paul; Fielding, Roger] Tufts Univ, Jean Mayer USDA HNRCA, Medford, MA USA. [McLean, Robert] Hebrew SeniorLife Inst Aging Res, Roslindale, MA USA. [Ramachandran, Vasan] Boston Univ, Sch Med, Framingham Heart Study, Boston, MA 02215 USA. [Mitchell, Gary] Cardiovasc Engn Inc, Norwood, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD FEB PY 2014 VL 29 SU 1 MA FR0448 BP S121 EP S121 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CK9ZS UT WOS:000356598701058 ER PT J AU Trombetti, A Reid, K Hars, M Herrmann, F Fielding, R AF Trombetti, Andrea Reid, Kieran Hars, Melany Herrmann, Francois Fielding, Roger TI Longitudinal Decline of Quality of Life is Determined by Loss of Muscle Mass and Reduced Physical Functioning in Older Adults. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the American-Society-for-Bone-and-Mineral-Research CY SEP 12-15, 2014 CL Houston, TX SP Amer Soc Bone & Mineral Res C1 [Trombetti, Andrea; Hars, Melany; Herrmann, Francois] Univ Hosp Geneva, Div Bone Dis, Geneva, Switzerland. [Trombetti, Andrea; Hars, Melany; Herrmann, Francois] Fac Med, Geneva, Switzerland. [Reid, Kieran; Fielding, Roger] Tufts Univ, Jean Mayer USDA HNRCA, Medford, MA USA. RI HERRMANN, Francois/B-6710-2011; Trombetti, Andrea/F-4575-2011 OI HERRMANN, Francois/0000-0003-1312-1517; NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD FEB PY 2014 VL 29 SU 1 MA SU0445 BP S343 EP S343 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CK9ZS UT WOS:000356598702132 ER PT J AU Boddicker, NJ Garrick, DJ Rowland, RRR Lunney, JK Reecy, JM Dekkers, JCM AF Boddicker, N. J. Garrick, D. J. Rowland, R. R. R. Lunney, J. K. Reecy, J. M. Dekkers, J. C. M. TI Validation and further characterization of a major quantitative trait locus associated with host response to experimental infection with porcine reproductive and respiratory syndrome virus SO ANIMAL GENETICS LA English DT Article DE genome wide association; porcine reproductive and respiratory syndrome; swine chromosome 4; validation ID PIGS AB Infectious diseases are costly to the swine industry; porcine reproductive and respiratory syndrome (PRRS) is the most devastating. In earlier work, a quantitative trait locus associated with resistance/susceptibility to PRRS virus was identified on Sus scrofa chromosome 4 using approximately 560 experimentally infected animals from a commercial cross. The favorable genotype was associated with decreased virus load and increased weight gain (WG). The objective here was to validate and further characterize the association of the chromosome 4 region with PRRS resistance using data from two unrelated commercial crossbred populations. The validation populations consisted of two trials each of approximately 200 pigs sourced from different breeding companies that were infected with PRRS virus and followed for 42days post-infection. Across all five trials, heritability estimates were 0.39 and 0.34 for viral load (VL; area under the curve of log-transformed viremia from 0 to 21days post-infection) and WG to 42days post-infection respectively. Effect estimates of SNP WUR10000125 in the chromosome 4 region were in the same directions and of similar magnitudes in the two new trials as had been observed in the first three trials. Across all five trials, the 1-Mb region on chromosome 4 explained 15percent of genetic variance for VL and 11percent for WG. The effect of the favorable minor allele at SNP WUR10000125 was dominant. Ordered genotypes for SNP WUR10000125 showed that the effect was present irrespective of whether the favorable allele was paternally or maternally inherited. These results demonstrate that selection for host response to PRRS virus infection could reduce the economic impact of PRRS. C1 [Boddicker, N. J.; Garrick, D. J.; Reecy, J. M.; Dekkers, J. C. M.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Garrick, D. J.] Massey Univ, Inst Vet Anim & Biomed Sci, Palmerston North 4474, New Zealand. [Rowland, R. R. R.] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA. [Lunney, J. K.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Dekkers, JCM (reprint author), Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. EM jdekkers@iastate.edu OI Garrick, Dorian/0000-0001-8640-5372 FU USDA NIFA PRRS CAP Award [2008-55620-19132]; National Pork Board; NRSP-8 Swine Genome and Bioinformatics Coordination projects; PRRS Host Genetics Consortium of USDA ARS; PRRS Host Genetics Consortium of Kansas State Univ.; PRRS Host Genetics Consortium of Iowa State Univ.; PRRS Host Genetics Consortium of Michigan State Univ.; PRRS Host Genetics Consortium of Washington State Univ.; PRRS Host Genetics Consortium of Purdue Univ.; PRRS Host Genetics Consortium of University of Nebraska-Lincoln; PRRS Host Genetics Consortium of PIC/Genus; PRRS Host Genetics Consortium of Newsham Choice Genetics; PRRS Host Genetics Consortium of Fast Genetics; PRRS Host Genetics Consortium of Genetiporc, Inc.; PRRS Host Genetics Consortium of Genesus, Inc.; PRRS Host Genetics Consortium of PigGen Canada, Inc.; PRRS Host Genetics Consortium of IDEXX Laboratories; PRRS Host Genetics Consortium of Tetracore, Inc. FX This project was supported by the USDA NIFA PRRS CAP Award 2008-55620-19132, the National Pork Board, the NRSP-8 Swine Genome and Bioinformatics Coordination projects, and the PRRS Host Genetics Consortium consisting of USDA ARS, Kansas State Univ., Iowa State Univ., Michigan State Univ., Washington State Univ., Purdue Univ., University of Nebraska-Lincoln, PIC/Genus, Newsham Choice Genetics, Fast Genetics, Genetiporc, Inc., Genesus, Inc., PigGen Canada, Inc., IDEXX Laboratories, Tetracore, Inc. The authors acknowledge technical assistance to this project from Juan Pedro Steibel for the algorithm to compute area under the curve, Max Rothschild for assistance with genotyping, Nader Deeb for assistance with data and analysis, Eric Fritz for database management, Ania Wolc and Dinesh Thekkoot for statistical assistance, the laboratory of Bob Rowland, specifically Becky Eaves, Maureen Kerrigan, Ben Trible, Jessica Otradovec, Brooke Bloomberg, Aubree Gottlob, Laura O'Brien and Ranjini Chand for animal care and sample collection, and the laboratory of Joan Lunney, specifically Samuel Abrams and Amber Tietgens for preparation of all genomic DNA samples for SNP genotyping. NR 18 TC 15 Z9 15 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD FEB PY 2014 VL 45 IS 1 BP 48 EP 58 DI 10.1111/age.12079 PG 11 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA 286HC UT WOS:000329456900006 PM 23914972 ER PT J AU Trott, JF Freking, BA Hovey, RC AF Trott, Josephine F. Freking, Bradley A. Hovey, Russell C. TI Variation in the coding and 3 ' untranslated regions of the porcine prolactin receptor short form modifies protein expression and function SO ANIMAL GENETICS LA English DT Article DE dominant negative; PRE-1; short interspersed repetitive element; single nucleotide polymorphisms ID RNA SECONDARY STRUCTURE; MAMMARY-GLAND; SIGNAL-TRANSDUCTION; MESSENGER-RNA; STRUCTURE PREDICTION; LACTATING SOWS; OVULATION RATE; BINDING-SITE; PRL RECEPTOR; LITTER SIZE AB The actions of prolactin (PRL) are mediated by both long (LF) and short isoforms (SF) of the PRL receptor (PRLR). Here, we report on a genetic and functional analysis of the porcine PRLR (pPRLR) SF. Three single nucleotide polymorphisms (SNPs) within exon 11 of the pPRLR-SF give rise to four amino acid haplotypes of the intracellular domain. We identified the dimorphic insertion of a short interspersed repetitive DNA element (PRE-1) along with 32 SNPs and four other insertion/deletion sites within the 3 untranslated region (UTR) of pPRLR-SF. The PRE-1 element reduced protein translation in vitro by 75%, whereas the combination of 10 SNPs and one insertion/deletion decreased translation by 50%. Full-length cDNAs for all four haplotypes of pPRLR-SF were cloned behind the elongation factor 1-alpha promoter and functionally analyzed in vitro. None of the haplotypes could initiate transcription from the ss-casein promoter, whereas all four were dominant negatives against PRL-activation of the pPRLR-LF. Two of the haplotypes completely inhibited pPRLR-LF activity at a four-fold excess, whereas the others required a six-fold excess to impart the same effect. The ligand binding affinities of the pPRLR-SF haplotypes did not differ. Expression of the pPRLR-SF increased linearly during gestation in the endometrium and was hormonally regulated in a tissue-specific manner in the mammary glands and uterus. In conclusion, we identified a PRE-1 and other SNPs in the pPRLR-SF 3 UTR that reduce protein expression and four haplotypes of the pPRLR-SF that suppress pPRLR-LF signaling and may differentially impact the phenotypic effects of PRL in vivo. C1 [Trott, Josephine F.; Hovey, Russell C.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. [Freking, Bradley A.] USDA ARS, Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Trott, JF (reprint author), Univ Calif Davis, Dept Anim Sci, 2335 Meyer Hall,1 Shields Ave, Davis, CA 95616 USA. EM jftrott@ucdavis.edu FU National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2008-35206-18895]; National Research Initiative Competitive Grant from the USDA Cooperative State Research, Education, and Extension Service [2004-35206-14140] FX This project was supported by the National Research Initiative Competitive Grant No. 2008-35206-18895 from the USDA National Institute of Food and Agriculture and by National Research Initiative Competitive Grant No. 2004-35206-14140 from the USDA Cooperative State Research, Education, and Extension Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 52 TC 5 Z9 5 U1 0 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD FEB PY 2014 VL 45 IS 1 BP 74 EP 86 DI 10.1111/age.12100 PG 13 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA 286HC UT WOS:000329456900009 PM 24350599 ER PT J AU Kim, ES Sonstegard, TS Silva, MVGB Gasbarre, LC Van Tassell, CP AF Kim, Eui-Soo Sonstegard, Tad S. Silva, Marcos Vinicius G. B. Gasbarre, Louis C. Van Tassell, Curtis P. TI Identification of quantitative trait loci affecting gastrointestinal parasite resistance in an experimental Angus population SO ANIMAL GENETICS LA English DT Article DE bovine; fecal egg count; gastrointestinal nematodes; quantitative trait loci ID NEMATODES; SHEEP; INFECTION; CATTLE; GENOME; QTL; SYSTEM; SCAN AB DNA markers associated with quantitative trait loci (QTL) affecting host tolerance to gastrointestinal (GI) parasite infection are ideal targets for marker-assisted selection. However, few studies in cattle have attempted to identify this type of QTL due to the difficulty of generating accurate phenotypic data from a resource population with adequate statistical power for detection. For this effort, we amassed fecal egg count (FEC) measures from annual natural field challenges with GI nematodes that spanned 12 different contemporary groups of Angus calves (1992-2000) derived from a closed breeding population. FEC and blood pepsinogen measures were taken weekly over a 26-week period post-weaning, and the FEC data were Box-Cox transformed to normalize the distribution of phenotypes. These 305 test animals and more than 100 founding animals from the extended pedigree were genotyped across 190 microsatellites markers. The genome-wide analyses identified a suggestive genome-wide QTL on bovine chromosome (Chr) 8 (P<0.002) and nominal QTL on Chr 4, 12 and 17 (P<0.05). These findings were unique for cattle, and some corresponded to previously identified QTL locations for parasite-related traits in sheep to provide genome locations for further fine mapping of parasite resistance/susceptibility in Angus cattle. C1 [Kim, Eui-Soo; Sonstegard, Tad S.; Silva, Marcos Vinicius G. B.; Gasbarre, Louis C.; Van Tassell, Curtis P.] USDA ARS, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. [Silva, Marcos Vinicius G. B.] Embrapa Dairy Cattle Ctr, BR-36038330 Juiz De Fora, MG, Brazil. RP Sonstegard, TS (reprint author), USDA ARS, Beltsville Agr Res Ctr, 10300 Baltimore Ave,Bldg 200 Rm 6 BARC East, Beltsville, MD 20705 USA. EM tad.sonstegard@ars.usda.gov OI Van Tassell, Curtis/0000-0002-8416-2087 FU USDA-ARS [1265-31000-098-00, 084-00] FX We thank Dr. E.C. Connor and the University of Maryland's Wye Facility for sharing material for genomic DNA extractions. We thank D. Herbert, T. Padilha and L. Shade for technical assistance. This work was supported by in part by Projects 1265-31000-098-00 and 084-00 from USDA-ARS. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 18 TC 2 Z9 2 U1 1 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD FEB PY 2014 VL 45 IS 1 BP 117 EP 121 DI 10.1111/age.12101 PG 5 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA 286HC UT WOS:000329456900015 PM 24303892 ER PT J AU Nonneman, D Lents, C Rohrer, G Rempel, L Vallet, J AF Nonneman, D. Lents, C. Rohrer, G. Rempel, L. Vallet, J. TI Genome-wide association with delayed puberty in swine SO ANIMAL GENETICS LA English DT Article DE puberty; SNP association; swine ID PERFORMANCE; EXPRESSION; PARITIES; LINES; GILTS; MICE; AGE AB An improvement in the proportion of gilts entering the herd that farrow a litter would increase overall herd performance and profitability. A significant proportion (10-30%) of gilts that enter the herd never farrow a litter; reproductive reasons account for approximately a third of gilt removals, with anestrous and failure to conceive the most common reasons for culling. Tools to select gilts for reproductive longevity through genomics or alternative phenotypes would be of great benefit to the producer. Ninety-one gilts that failed to display behavioral estrus by 240days (cases) and 127 pubertal littermates (controls) were genotyped with the Illumina Porcine SNP60 Beadchip. One hundred and seventy-four SNPs with the most significant associations were genotyped in an additional 86 cases and 103 controls. Twelve of these associations were significant in the final analysis. The most significant (P<1.5x10(-14)) region associated with failure to attain puberty was on chromosome 4 surrounding the NHLH2 gene. Delayed pubertal development and age at first estrus have been associated with NHLH2 in mice. Because attainment of puberty is a complex trait, identifying genes that affect pubertal age would greatly contribute to our knowledge of reproductive development as well as overall fertility. C1 [Nonneman, D.; Lents, C.; Rohrer, G.; Rempel, L.; Vallet, J.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Nonneman, D (reprint author), USDA ARS, US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. EM dan.nonneman@ars.usda.gov NR 17 TC 2 Z9 2 U1 1 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD FEB PY 2014 VL 45 IS 1 BP 130 EP 132 DI 10.1111/age.12087 PG 3 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA 286HC UT WOS:000329456900018 PM 23980905 ER PT J AU Banks, ML Kennedy, AC Krerner, RJ Eivazi, F AF Banks, M. L. Kennedy, A. C. Krerner, R. J. Eivazi, F. TI Soil microbial community response to surfactants and herbicides in two soils SO APPLIED SOIL ECOLOGY LA English DT Article DE PLFA; Adjuvant; Surfactant; Herbicides; Texture; Soil microbial community ID FATTY-ACID PROFILES; UTILIZATION PATTERNS; FUNGAL BIOMASS; LIPID ANALYSIS; BACTERIAL; GLYPHOSATE; DEGRADATION; RHIZOSPHERE; GRASSLAND; PHOSPHOLIPIDS AB The environmental impacts of herbicides on desirable plants and the soil biota. are of public concern. The surfactants that are often used with herbicides are also under scrutiny as potentially harmful to soil biological systems. TO address these concerns, we used two soils, a silt loam and a silty, clay loam from south central Missouri, to investigate the impacts of herbicides and surfactants on soil microbial communities using phospholipid fatty acid (PLFA) analysis. The surfactants used in this study were alkylphenol ethoxylate plus alcohol ethoxylate (Activator 90), polyethoxylate (Agri-Dex), and a blend of ammonium sulfate, drift reduction/deposition polymers and anti-foam agent (Thrust). The herbicides were glyphosate, atrazine and bentazon. Surfactants and herbicides were applied to soils at label rate, either alone or combined, to 4000g soil per pot. The two soils differed in history, texture, some chemical characteristics and several microbial community characteristics. A few of the chemicals altered some of the components of the microbial community after only one application of the chemical at field-rate. The Cole County, MO silt loam showed larger changes in the microbial community with application of treatments. For the Boone County, MO silty clay loam, Activator 90, Agri-Dex and bentazon treatments increased microbial biomass determined by PLFA; Thrust decreased PLFA markers, bacteria to fungi ratio; and Agri-Dex at both rates decreased monounsaturated fatty acids. Changes in the microbial community due to herbicides or surfactants were minimal in this study of a single application of these chemicals, but could be indicators of potential long-term effects. Long-term studies are needed to determine the changes in the microbial community after several years of annual applications of herbicides and surfactants on a wide array of soil types and management practices. Published by Elsevier B.V. C1 [Banks, M. L.] Univ Missouri, Dept Soil Environm & Atmospher Sci, Columbia, MO 65211 USA. [Kennedy, A. C.] Washington State Univ, USDA ARS, Land Management & Water Conservat Res Unit, Pullman, WA 99164 USA. [Krerner, R. J.] Univ Missouri, USDA ARS, Cropping Syst & Water Qual Unit, Columbia, MO 65211 USA. [Eivazi, F.] Lincoln Univ Missouri, Jefferson City, MO 65101 USA. RP Banks, ML (reprint author), USDA ARS, Greenhouse Prod Res Grp, 2801 W Bancroft St, Toledo, OH 43606 USA. EM mona-lisa.banks@ars.usda.gov; ann.kennedy@ars.usda.gov NR 81 TC 9 Z9 11 U1 7 U2 113 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD FEB PY 2014 VL 74 BP 12 EP 20 DI 10.1016/j.apsoil.2013.08.018 PG 9 WC Soil Science SC Agriculture GA 285TY UT WOS:000329419100002 ER PT J AU Boone, CG Fragkias, M Buckley, GL Grove, JM AF Boone, Christopher G. Fragkias, Michail Buckley, Geoffrey L. Grove, J. Morgan TI A long view of polluting industry and environmental justice in Baltimore SO CITIES LA English DT Article DE Environmental justice; Baltimore; Longitudinal; Toxics Release Inventory; Hazards Density Index; Sustainability ID SPATIAL-DISTRIBUTION; RACIAL-INEQUALITY; UNITED-STATES; RACE; INEQUITY; HAZARDS; EQUITY; HEALTH; WASTE; RISK AB Purpose: This study examines the density of polluting industry by neighborhoods in Baltimore over the long term, from 1950 to 2010, to determine if high pollution burdens correspond spatially with expected demographic and housing variables predicted in the environmental justice literature. For 1960-1980 we use data on heavy industry from Dun and Bradstreet directories and for 1990-2010 the US EPA's Toxics Release Inventory to calculate a Hazards, Density Index. Drawing on the decennial censuses for 1960-2010, we populate census tracts from corresponding years with data on race, ethnicity, educational attainment, income, and housing tenure. Findings: Density of polluting industry is positively correlated with low-income neighborhoods and renter-occupied housing in 1960 and by 2010 with white, Hispanic, and low educational attainment populations. In general, over time density of polluting facilities shifts from an association with wealth to race and ethnicity while educational attainment remains a significant variable throughout. This study confirms earlier analyses on Baltimore that white neighborhoods are more likely than African-American neighborhoods (1990-2010) to contain polluting facilities but reveals for the first time that educational attainment is also significant. The paper concludes with a discussion of the Baltimore Sustainability Plan and its weak efforts to address persistent environmental injustices. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Boone, Christopher G.] Arizona State Univ, Sch Sustainabil, Tempe, AZ 85287 USA. [Fragkias, Michail] Boise State Univ, Dept Econ, Boise, ID 83725 USA. [Buckley, Geoffrey L.] Ohio Univ, Dept Geog, Athens, OH 45701 USA. [Grove, J. Morgan] US Forest Serv, Baltimore Field Stn, No Res Stn, Baltimore, MD 21228 USA. [Grove, J. Morgan] US Forest Serv, Baltimore Ecosyst Study, No Res Stn, Baltimore, MD 21228 USA. RP Boone, CG (reprint author), Arizona State Univ, Sch Sustainabil, POB 875502, Tempe, AZ 85287 USA. EM cgboone@asu.edu; michailfragkias@boisestate.e-du; buckleyg@ohio.edu; mgrove@fs.fed.us NR 45 TC 8 Z9 8 U1 5 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-2751 EI 1873-6084 J9 CITIES JI Cities PD FEB PY 2014 VL 36 SI SI BP 41 EP 49 DI 10.1016/j.cities.2013.09.004 PG 9 WC Urban Studies SC Urban Studies GA 285IA UT WOS:000329386100005 ER PT J AU Richards, TJ Mancino, L AF Richards, Timothy J. Mancino, Lisa TI Demand for food-away-from-home: a multiple-discrete-continuous extreme value model SO EUROPEAN REVIEW OF AGRICULTURAL ECONOMICS LA English DT Article DE discrete-continuous models; food-away-from-home; obesity; taxation ID RESTAURANT USE; WORKING WIVES; WEIGHT-GAIN; TIME-USE; OBESITY; CONSUMPTION; REGRESSION; ECONOMETRICS; INSTRUMENTS; PREFERENCES AB Policymakers have suggested the use of taxes to raise the relative cost of buying fast food. Yet, little is known of the structure of demand for food-away-from-home (FAFH) in general. This study provides estimates of the price-elasticity of demand for four different types of FAFH using a new data set from NPD, Inc. and an econometric approach that accounts for the multiple-discretecontinuous nature of FAFH demand. We find that cross-price elasticities of demand are small, so consumers are unwilling to substitute between food-at-home and any type of FAFH or among types of FAFH. Therefore, taxing fast food may be effective in reducing the number of fast food visits and shifting consumption to at-home meals. C1 [Richards, Timothy J.; Mancino, Lisa] Arizona State Univ, Tempe, AZ 85287 USA. [Richards, Timothy J.; Mancino, Lisa] Econ Res Serv, USDA, Washington, DC USA. RP Richards, TJ (reprint author), 7231 E Sonoran Arroyo Mall, Mesa, AZ 85212 USA. EM trichards@asu.edu FU Economic Research Service of the USDA FX Support from the Economic Research Service of the USDA and excellent comments from the reviewers and editor are gratefully acknowledged. NR 47 TC 0 Z9 0 U1 3 U2 13 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0165-1587 EI 1464-3618 J9 EUR REV AGRIC ECON JI Eur. Rev. Agric. Econ. PD FEB PY 2014 VL 41 IS 1 BP 111 EP 133 DI 10.1093/erae/jbt008 PG 23 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 281WY UT WOS:000329132900006 ER PT J AU Tatineni, S French, R AF Tatineni, Satyanarayana French, Roy TI The C-terminus of Wheat streak mosaic virus Coat Protein Is Involved in Differential Infection of Wheat and Maize through Host-Specific Long-Distance Transport SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID CELL-TO-CELL; HELPER COMPONENT-PROTEINASE; TOBACCO ETCH POTYVIRUS; ERIOPHYID MITE TRANSMISSION; PLUM-POX-VIRUS; CAPSID PROTEIN; PLANT-VIRUSES; HC-PRO; SYSTEMIC INFECTION; MOVEMENT PROTEIN AB Viral determinants and mechanisms involved in extension of host range of monocot-infecting viruses are poorly understood. Viral coat proteins (CP) serve many functions in almost every aspect of the virus life cycle. The role of the C-terminal region of Wheat streak mosaic virus (WSMV) CP in virus biology was examined by mutating six negatively charged aspartic acid residues at positions 216, 289, 290, 326, 333, and 334. All of these amino acid residues are dispensable for virion assembly, and aspartic acid residues at positions 216, 333, and 334 are expendable for normal infection of wheat and maize. However, mutants D289N, D(289)A, D(290)A, DD(289/290)NA, and D(326)A exhibited slow cell-to-cell movement in wheat, which resulted in delayed onset of systemic infection, followed by a rapid recovery of genomic RNA accumulation and symptom development. Mutants D289N, D(289)A, and D(326)A inefficiently infected maize, eliciting milder symptoms, while D(290)A and DD(289/290)NA failed to infect systemically, suggesting that the C-terminus of CP is involved in differential infection of wheat and maize. Mutation of aspartic acid residues at amino acid positions 289, 290, and 326 severely debilitated virus ingress into the vascular system of maize but not wheat, suggesting that these amino acids facilitate expansion of WSMV host range through host-specific long-distance transport. C1 [Tatineni, Satyanarayana] Univ Nebraska, ARS, USDA, Lincoln, NE 68583 USA. Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Tatineni, S (reprint author), Univ Nebraska, ARS, USDA, Lincoln, NE 68583 USA. EM satya.tatineni@ars.usda.gov NR 76 TC 4 Z9 4 U1 1 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD FEB PY 2014 VL 27 IS 2 BP 150 EP 162 PG 13 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA 285QG UT WOS:000329408100006 PM 24111920 ER PT J AU Rao, SS El-Habbak, MH Havens, WM Singh, A Zheng, DM Vaughn, L Haudenshield, JS Hartman, GL Korban, SS Ghabrial, SA AF Rao, Suryadevara S. El-Habbak, Mohamed H. Havens, Wendy M. Singh, Ajay Zheng, Danman Vaughn, Laura Haudenshield, James S. Hartman, Glen L. Korban, Schuyler S. Ghabrial, Said A. TI Overexpression of GmCaM4 in soybean enhances resistance to pathogens and tolerance to salt stress SO MOLECULAR PLANT PATHOLOGY LA English DT Article ID SYSTEMIC ACQUIRED-RESISTANCE; CALMODULIN-BINDING PROTEINS; POD-MOTTLE-VIRUS; GENE-EXPRESSION; TRANSCRIPTION FACTORS; ARABIDOPSIS NPR1; MOSAIC-VIRUS; PLANT; CALCIUM; LOCALIZATION AB Plant diseases inflict heavy losses on soybean yield, necessitating an understanding of the molecular mechanisms underlying biotic/abiotic stress responses. Ca2+ is an important universal messenger, and protein sensors, prominently calmodulins (CaMs), recognize cellular changes in Ca2+ in response to diverse signals. Because the development of stable transgenic soybeans is laborious and time consuming, we used the Bean pod mottle virus (BPMV)-based vector for rapid and efficient protein expression and gene silencing. The present study focuses on the functional roles of the gene encoding the soybean CaM isoform GmCaM4. Overexpression of GmCaM4 in soybean resulted in enhanced resistance to three plant pathogens and increased tolerance to high salt conditions. To gain an understanding of the underlying mechanisms, we examined the potential defence pathways involved. Our studies revealed activation/increased expression levels of pathogenesis-related (PR) genes in GmCaM4-overexpressing plants and the accumulation of jasmonic acid (JA). Silencing of GmCaM4, however, markedly repressed the expression of PR genes. We confirmed the invivo interaction between GmCaM4 and the CaM binding transcription factor Myb2, which regulates the expression of salt-responsive genes, using the yeast two-hybrid (Y2H) system and bimolecular fluorescence complementation assays. GmCaM4 and Glycine maxCaM binding receptor-like kinase (GmCBRLK) did not interact in the Y2H assays, but the interaction between GmCaM2 and GmCBRLK was confirmed. Thus, a GmCaM2-GmCBRLK-mediated salt tolerance mechanism, similar to that reported in Glycine soja, may also be functional in soybean. Confocal microscopy showed subcellular localization of the green fluorescent protein (GFP)-GmCaM4 fusion protein in the nucleus and cytoplasm. C1 [Rao, Suryadevara S.; El-Habbak, Mohamed H.; Havens, Wendy M.; Singh, Ajay; Ghabrial, Said A.] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Zheng, Danman; Vaughn, Laura; Korban, Schuyler S.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61821 USA. [Haudenshield, James S.; Hartman, Glen L.] ARS, USDA, Urbana, IL 61801 USA. [Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Ghabrial, SA (reprint author), Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. EM saghab00@email.uky.edu FU United Soybean Board [0262]; Kentucky Science and Engineering Foundation FX This project was funded in part by a grant received from the United Soybean Board (grant #0262 to SSK, SAG and GLH) and by the Kentucky Science and Engineering Foundation (grant # to SAG). We thank A. Crume for maintaining glasshouse plants. NR 55 TC 10 Z9 15 U1 4 U2 65 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 EI 1364-3703 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD FEB PY 2014 VL 15 IS 2 BP 145 EP 160 DI 10.1111/mpp.12075 PG 16 WC Plant Sciences SC Plant Sciences GA 284DW UT WOS:000329297300004 PM 24118726 ER PT J AU Wallis, CM Rashed, A Wallingford, AK Paetzold, L Workneh, F Rush, CM AF Wallis, C. M. Rashed, A. Wallingford, A. K. Paetzold, L. Workneh, F. Rush, C. M. TI Similarities and Differences in Physiological Responses to 'Candidatus Liberibacter solanacearum' Infection Among Different Potato Cultivars SO PHYTOPATHOLOGY LA English DT Article DE bacteria ID ZEBRA CHIP DISEASE; UNITED-STATES; 1ST REPORT; TUBERS; ACRYLAMIDE; PLANTS; EPIDEMIOLOGY; BIOCHEMISTRY; ASSOCIATION; MANAGEMENT AB Zebra chip disease (ZC), putatively caused by the fastidious bacterium 'Candidatus Liberibacter solanacearum', is a threat to potato growers worldwide. However, little is known about biochemical shifts in different potato genotypes in response to 'Ca. L. solanacearum' infection. To address this, 'Red La Soda', 'Russet Norkotah', and 'FL 1867' potato were infected with 'Ca. L. solanacearum' 4, 3, 2, and 1 weeks before harvest to observe variability in cultivar responses to 'Ca. L. solanacearum' infection. ZC symptoms, 'Ca. L. solanacearum' titers, and tuber biochemistry were assessed. Red La Soda tubers exhibited greater symptoms when infected for 4 weeks than Russet Norkotah or FL 1867 tubers. 'Ca. L. solanacearum' titers did not vary among cultivars. Tuber levels of amino acids, carbohydrates, and phenolics varied among cultivars but no consistent trends were observed. Individual amino acids and phenolics were greater in FL 1867 than Red La Soda, whereas others were greater in Red La Soda or Russet Norkotah than FL 1867. Most amino acids, carbohydrates, and phenolics were positively associated with infection duration and symptoms regardless of cultivar. Associations between most of the evaluated compounds and 'Ca. L. solanacearum' titer were positive in Red La Soda. However, no associations between 'Ca. L. solanacearum' quantity and compounds were observed in FL 1867 and Russet Norkotah. C1 [Wallis, C. M.; Wallingford, A. K.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Rashed, A.; Paetzold, L.; Workneh, F.; Rush, C. M.] Texas A&M AgriLife Res, Amarillo, TX 79106 USA. RP Wallis, CM (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM christopher.wallis@ars.usda.gov FU ZC SCRI/Industry; master ZC SCRI project, a USDA Specialty Crop Research Initiative grant [2009-51181-20176] FX This research was supported by a ZC SCRI/Industry-Funded Mini-Grant, as well as the master ZC SCRI project, a USDA Specialty Crop Research Initiative grant (number 2009-51181-20176). We thank J. Gray, G. Aguilar, A. Fite, N. Goodell, and M. Henning for their assistance in the execution of this work; and Barrett's Potato Farms and CSS Farms for providing seed potato. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 34 TC 5 Z9 6 U1 2 U2 18 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2014 VL 104 IS 2 BP 126 EP 133 DI 10.1094/PHYTO-05-13-0125-R PG 8 WC Plant Sciences SC Plant Sciences GA 285YK UT WOS:000329430800002 PM 23941779 ER PT J AU Ma, W Liang, M Guan, L Xu, M Wen, X Deng, X Chen, J AF Ma, W. Liang, M. Guan, L. Xu, M. Wen, X. Deng, X. Chen, J. TI Population Structures of 'Candidatus Liberibacter asiaticus' in Southern China SO PHYTOPATHOLOGY LA English DT Article ID HUANGLONGBING BACTERIUM; TANDEM REPEATS; CITRUS; GUANGDONG; SEQUENCE; DISEASE; GENOME; LOCUS AB Huanglongbing (HLB) is a highly detrimental citrus disease associated with 'Candidatus Liberibacter asiaticus', a nonculturable alpha-proteobacterium. Characterization of the bacterial populations is important for development of disease management strategies. In this study, the 'Ca. L. asiaticus' populations in eight provinces in southern China where HLB is endemic were analyzed based on tandem repeat number (TRN) variations in a previously characterized genomic locus CLIBASIA_01645. Of the 224 HLB samples collected, 175 (78.3%) samples yielded single polymerase chain reaction (PCR) amplicons (the single amplicon group, SAG) and 49 (21.7%) samples produced multiple PCR amplicons (the multiple amplicon group, MAG). Variations in SAG are summarized by Nei's diversity index (H) and ratio of TRN <= 10/TRN > 10 genotypes (R-10). Variations in the MAG are described by the percentage of occurrence (P-MAG). At an orchard-level comparison, the 'Ca. L. asiaticus' population from a Guangdong orchard (n = 24) showed H = 0.50, R-10 = 23, and P-MAG = 0, significantly different from that of the non-Guangdong orchards in Yunnan (n = 23), H = 0.83, R-10 = 2.3, and P-MAG = 11.5, and in Hainan (n = 35), H = 0.88, R-10 = 1.5, and P-MAG = 16.7. In a region-level consideration, the Guangdong 'Ca. L. asiaticus' population (n = 78) was H = 0.77, R-10 = 25, and P-MAG = 1.3, whereas the non-Guangdong population (n = 84) was H = 0.91, R-10 = 1.6, and P-MAG = 26.9. Overall, significant differences were observed between the 'Ca. L. asiaticus' population from Guangdong Province and those from the other provinces. A strong aggregation of TRN = 6, 7, and 8 genotypes is characteristic to the 'Ca. L. asiaticus' population in Guangdong. Referenced to genome annotation, we propose that rearrangement of tandem repeats at locus CLIBASIA_01645 could be associated with bacterial environmental adaptation. C1 [Ma, W.; Liang, M.; Guan, L.; Xu, M.; Wen, X.; Deng, X.] South China Agr Univ, Dept Plant Pathol, Lab Citrus Huanglongbing Res, Guangzhou, Guangdong, Peoples R China. [Chen, J.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Crop Dis Pests & Genet Res Unit, Parlier, CA USA. RP Deng, X (reprint author), South China Agr Univ, Dept Plant Pathol, Lab Citrus Huanglongbing Res, Guangzhou, Guangdong, Peoples R China. EM xldeng@scau.edu.cn; jianchi.chen@ars.usda.gov FU Chinese Modern Agricultural Technology Systems [CARS-27]; Special Fund for Agro-Scientific Research in the Public Interest, China [2010003067]; California Citrus Research Board FX This research was supported by Chinese Modern Agricultural Technology Systems (CARS-27) and the Special Fund for Agro-Scientific Research in the Public Interest, China (2010003067). California Citrus Research Board also provided partial support. We thank C. Wallis for his suggestion and editing of this manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 21 TC 6 Z9 11 U1 2 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2014 VL 104 IS 2 BP 158 EP 162 DI 10.1094/PHYTO-04-13-0110-R PG 5 WC Plant Sciences SC Plant Sciences GA 285YK UT WOS:000329430800006 PM 24093922 ER PT J AU Schoettle, AW Sniezko, RA Kegley, A Burns, KS AF Schoettle, A. W. Sniezko, R. A. Kegley, A. Burns, K. S. TI White Pine Blister Rust Resistance in Limber Pine: Evidence for a Major Gene SO PHYTOPATHOLOGY LA English DT Article ID CRONARTIUM-RIBICOLA; SUGAR PINE; HYPERSENSITIVE RESPONSE; NATURAL-POPULATIONS; RANGE; COLORADO AB Limber pine (Pinus flexilis) is being threatened by the lethal disease white pine blister rust caused by the non-native pathogen Cronartium ribicola. The types and frequencies of genetic resistance to the rust will likely determine the potential success of restoration or proactive measures. These first extensive inoculation trials using individual tree seed collections from >100 limber pine trees confirm that genetic segregation of a stem symptom-free trait to blister rust is consistent with inheritance by a single dominant resistance (R) gene, and the resistance allele appears to be distinct from the R allele in western white pine. Following previous conventions, we are naming the R gene for limber pine "Cr4." The frequency of the Cr4 allele across healthy and recently invaded populations in the Southern Rocky Mountains was unexpectedly high (5.0%, ranging from 0 to 13.9%). Cr4 is in equilibrium, suggesting that it is not a product of a recent mutation and may have other adaptive significance within the species, possibly related to other abiotic or biotic stress factors. The identification of Cr4 in native populations of limber pine early in the invasion progress in this region provides useful information for predicting near-term impacts and structuring long-term management strategies. C1 [Schoettle, A. W.] USDA, Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Sniezko, R. A.; Kegley, A.] USDA, Forest Serv, Dorena Genet Resource Ctr, Cottage Grove, OR 97424 USA. [Burns, K. S.] USDA, Forest Serv, Golden, CO 80401 USA. RP Schoettle, AW (reprint author), USDA, Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. EM aschoettle@fs.fed.us FU USDA Forest Service Special Technology Development Program [R2-2006-02]; USDI Park Service Great Sand Dunes National Park and Preserve [08-IA-11221633-304, 08-IA-11221633-305] FX This research was funded by an award from the USDA Forest Service Special Technology Development Program (R2-2006-02) and an agreement from USDI Park Service Great Sand Dunes National Park and Preserve (08-IA-11221633-304 and 08-IA-11221633-305). We thank R. Danchok, S. Long, B. Mayo, J. Hill, B. Luis, I. Lebouc, and K. Joy for assistance with rust assessments; L. Riley and the DGRC nursery crew for plant culture work; B. Kinloch, Jr. and P. Zambino for helpful discussions; P. Vogan for comments on an earlier version of the manuscript and two anonymous reviewers. A. Schoettle, R. Sniezko, and J. Hill provided the photos in Figures 2 and 4. NR 54 TC 13 Z9 13 U1 1 U2 26 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2014 VL 104 IS 2 BP 163 EP 173 DI 10.1094/PHYTO-04-13-0092-R PG 11 WC Plant Sciences SC Plant Sciences GA 285YK UT WOS:000329430800007 PM 23941780 ER PT J AU Schutte, BJ Tomasek, BJ Davis, AS Andersson, L Benoit, DL Cirujeda, A Dekker, J Forcella, F Gonzalez-Andujar, JL Graziani, F Murdoch, AJ Neve, P Rasmussen, IA Sera, B Salonen, J Tei, F Torresen, KS Urbano, JM AF Schutte, B. J. Tomasek, B. J. Davis, A. S. Andersson, L. Benoit, D. L. Cirujeda, A. Dekker, J. Forcella, F. Gonzalez-Andujar, J. L. Graziani, F. Murdoch, A. J. Neve, P. Rasmussen, I. A. Sera, B. Salonen, J. Tei, F. Torresen, K. S. Urbano, J. M. TI An investigation to enhance understanding of the stimulation of weed seedling emergence by soil disturbance SO WEED RESEARCH LA English DT Article DE tillage; seedbank management; stale seedbed; genotype-environment interactions; seed germination; Chenopodium album ID LAMBSQUARTERS CHENOPODIUM-ALBUM; RAGWEED AMBROSIA-TRIFIDA; DORMANCY; GERMINATION; TILLAGE; MODEL; PHOTOINDUCTION; RECRUITMENT; COMPACTION; ECOLOGY AB Enhanced understanding of soil disturbance effects on weed seedling recruitment will help guide improved management approaches. Field experiments were conducted at 16 site-years at 10 research farms across Europe and North America to (i) quantify superficial soil disturbance (SSD) effects on Chenopodium album emergence and (ii) clarify adaptive emergence behaviour in frequently disturbed environments. Each site-year contained factorial combinations of two seed populations (local and common, with the common population studied at all site-years) and six SSD timings [0, 50, 100, 150, 200day-degrees (d degrees C, base temperature 3 degrees C) after first emergence from undisturbed soil]. Analytical units in this study were emergence flushes. Flush magnitudes (maximum weekly emergence per countflush) and flush frequencies (flushesyear(-1)) were compared between disturbed and undisturbed seedbanks. One year after burial, SSD promoted seedling emergence relative to undisturbed seedbanks by increasing flush magnitude rather than increasing flush frequency. Two years after burial, SSD promoted emergence through increased flush magnitude and flush frequency. The promotional effects of SSD on emergence were strongest within 500d degrees C following SSD; however, low levels of SSD-induced emergence were detected as late as 3000d degrees C following SSD. Accordingly, stale seedbed practices that eliminate weed seedlings should occur within 500d degrees C of disturbance, because few seedlings emerge after this time. However, implementation of stale seedbed practices will probably cause slight increases in weed population densities throughout the year. Compared with the common population, local populations exhibited reduced variance in total emergence measured within sites and across SSD treatments, suggesting that C.album adaptation to local pedo-climatic conditions involves increased consistency in SSD-induced emergence. C1 [Schutte, B. J.; Tomasek, B. J.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Davis, A. S.] ARS, Photosynthesis & Global Change Res Unit, USDA, Urbana, IL USA. [Andersson, L.] Swedish Univ Agr Sci, Dept Crop Prod Ecol, Uppsala, Sweden. [Benoit, D. L.] Agr & AgriFood Canada, Hort Res & Dev Ctr, St Jean, PQ, Canada. [Cirujeda, A.] CITA, Unidad Sanidad Vegetal, Zaragoza, Spain. [Dekker, J.] Iowa State Univ, Dept Agron, Ames, IA USA. [Forcella, F.] ARS, N Cent Soil Conservat Res Lab, USDA, Morris, MN USA. [Gonzalez-Andujar, J. L.] Inst Agr Sostenible CSIC, Dpto Protecc Cultivos, Cordoba, Spain. [Graziani, F.; Tei, F.] Univ Perugia, Dept Agr & Environm Sci, I-06100 Perugia, Italy. [Murdoch, A. J.] Univ Reading, Sch Agr Policy & Dev, Reading, Berks, England. [Neve, P.] Univ Warwick, Sch Life Sci, Wellesbourne, Warwick, England. [Rasmussen, I. A.] ICROFS Blichers Alle, Int Ctr Res Organ Food Syst, Tjele, Denmark. [Sera, B.] Acad Sci Czech Republic, Inst Nanobiol & Struct Biol GCRC, Ceske Budejovice, Czech Republic. [Salonen, J.] MTT Agrifood Res Finland, Plant Prod Res, Jokioinen, Finland. [Torresen, K. S.] Bioforsk Norwegian Inst Agr & Environm Res, Plant Hlth & Plant Protect Div, As, Norway. [Urbano, J. M.] Univ Seville, EUTI Agr, Seville, Spain. RP Schutte, BJ (reprint author), New Mexico State Univ, Dept Entomol Plant Pathol & Weed Sci, 945 Coll Ave, Las Cruces, NM USA. EM bschutte@nmsu.edu RI Cirujeda, Alicia/J-1506-2012; Sera, Bozena/H-1327-2014; GONZALEZ-ANDUJAR, JOSE /G-5671-2013; Neve, Paul/P-8726-2015; OI Cirujeda, Alicia/0000-0001-9646-8422; GONZALEZ-ANDUJAR, JOSE /0000-0003-2356-4098; Neve, Paul/0000-0002-3136-5286; Andersson, Lars/0000-0002-1676-5361; TEI, Francesco/0000-0003-0857-9662 FU EWRS FX This study was conducted by the European Weed Research Society (EWRS) Working Group on Germination and Early Growth. We are grateful to the participating organisations, especially the EWRS, for providing funds in support of this working group. We are saddened by the passing of our colleague, Dr. E. Sousa, and we dedicate this article to her memory. NR 33 TC 7 Z9 7 U1 4 U2 52 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0043-1737 EI 1365-3180 J9 WEED RES JI Weed Res. PD FEB PY 2014 VL 54 IS 1 BP 1 EP 12 DI 10.1111/wre.12054 PG 12 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 284JM UT WOS:000329312400001 ER PT J AU Hull, JJ Perera, OP Snodgrass, GL AF Hull, J. J. Perera, O. P. Snodgrass, G. L. TI Cloning and expression profiling of odorant-binding proteins in the tarnished plant bug, Lygus lineolaris SO INSECT MOLECULAR BIOLOGY LA English DT Article DE Lygus lineolaris; odorant-binding proteins; olfaction; real-time PCR; Plus-C OBP; classic OBP; alternative splice variants; next-generation sequencing ID MOSQUITO ANOPHELES-GAMBIAE; PHEROMONE-SENSITIVE NEURONS; SOUTHERN HOUSE MOSQUITO; MISSISSIPPI RIVER DELTA; MALARIA VECTOR MOSQUITO; FEMALE SEX-PHEROMONE; GENE FAMILY; DROSOPHILA-MELANOGASTER; CULEX-QUINQUEFASCIATUS; CHEMOSENSORY PROTEINS AB In insects, the perception and discrimination of odorants requires the involvement of odorant-binding proteins (OBPs). To gain a better molecular understanding of olfaction in the agronomic pest Lygus lineolaris (the tarnished plant bug), we used a transcriptomics-based approach to identify potential OBPs. In total, 33 putative OBP transcripts, including the previously reported Lygus antennal protein (LAP), were identified based on the characteristic OBP Cys signature and/or sequence similarity with annotated orthologous sequences. The L.lineolarisOBP (LylinOBP) repertoire consists of 20 classic' OBPs, defined by the spacing of six conserved Cys residues, and 12 Plus-C' OBPs, defined by the spacing of eight conserved Cys and one conserved Pro residue. Alternative splicing of OBP genes appears to contribute significantly to the multiplicity of LylinOBP sequences. Microarray-based analysis of chemosensory tissues (antennae, legs and proboscis) revealed enrichment of 21 LylinOBP transcripts in antennae, 12 in legs, and 15 in proboscis, suggesting potential roles in olfaction and gustation respectively. PCR-based determination of transcript abundance for a subset of the LylinOBP genes across multiple adult tissues yielded results consistent with the hybridization data. C1 [Hull, J. J.] USDA ARS Arid Land Agr Ctr, Maricopa, AZ USA. [Perera, O. P.; Snodgrass, G. L.] USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. RP Hull, JJ (reprint author), USDA ARS Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM joe.hull@ars.usda.gov NR 93 TC 12 Z9 14 U1 5 U2 52 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD FEB PY 2014 VL 23 IS 1 BP 78 EP 97 DI 10.1111/imb.12064 PG 20 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 282JC UT WOS:000329166100007 PM 24224606 ER PT J AU Wheeler, DE Buck, NA Evans, JD AF Wheeler, D. E. Buck, N. A. Evans, J. D. TI Expression of insulin/insulin-like signalling and TOR pathway genes in honey bee caste determination SO INSECT MOLECULAR BIOLOGY LA English DT Article DE social insects; polyphenism; queen production; insect nutrition ID APIS-MELLIFERA-L; INSULIN-LIKE PEPTIDES; JUVENILE-HORMONE; GROWTH-CONTROL; DEVELOPMENTAL STAGES; DROSOPHILA; LARVAE; DIFFERENTIATION; INDUCTION; QUEEN AB The development of queen and worker castes in honey bees is induced by differential nutrition, with future queens and workers receiving diets that are qualitatively and quantitatively different. We monitored the gene expression of 14 genes for components of the insulin/insulin-like signalling and TOR pathways in honey bee larvae from 40-88h after hatching. We compared normally fed queen and normally fed worker larvae and found that three genes showed expression differences in 40-h-old larvae. Genes that show such early differences in expression may be part of the mechanism that transduces nutrition level into a hormone signal. We then compared changes in expression after shifts in diet with those in normally developing queens and workers. Following a shift to the worker diet, the expression of 9/14 genes was upregulated in comparison with queens. Following a shift to the queen diet, expression of only one gene changed. The honey bee responses may function together as a homeostatic mechanism buffering larvae from caste-disrupting variation in nutrition. The different responses would be part of the canalization of both the queen and worker developmental pathways, and as such, a signature of advanced sociality. C1 [Wheeler, D. E.] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. [Buck, N. A.] Univ Arizona, Tucson, AZ 85721 USA. [Evans, J. D.] USDA ARS, Bee Res Lab, Beltsville, MD USA. RP Wheeler, DE (reprint author), Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. EM dewsants@ag.arizona.edu RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 FU NSF [IAB 0344946] FX Funding was provided by NSF grant IAB 0344946 to DEW. We thank the talented Dawn Lopez for transferring larvae and Mike Reilly for this comments on an earlier version of the manuscript. DEW also thanks the two anonymous reviewers whose thoughtful and thorough reviews define constructive criticism. NR 31 TC 7 Z9 9 U1 5 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD FEB PY 2014 VL 23 IS 1 BP 113 EP 121 DI 10.1111/imb.12065 PG 9 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 282JC UT WOS:000329166100009 PM 24224645 ER PT J AU Abrams, SA Tiosano, D AF Abrams, Steven A. Tiosano, Dov TI Update on vitamin D during childhood SO CURRENT OPINION IN ENDOCRINOLOGY DIABETES AND OBESITY LA English DT Review DE calcium metabolism; hypercalcium; rickets; vitamin D; vitamin D receptor ID 25-HYDROXYVITAMIN D LEVELS; D-BINDING PROTEIN; INFANTILE HYPERCALCEMIA; CALCIUM-ABSORPTION; RESISTANT RICKETS; CHILDREN; 1,25-DIHYDROXYVITAMIN-D; DENSITY; CYP24A1; ASTHMA AB Purpose of reviewWe propose to review several recent key clinically oriented topics related to vitamin D and health in children.Recent findingsWe found a very large number of recent clinical studies related to vitamin D. However, most are association studies with few physiological or clinical trials that are adequately powered for clinical outcomes. Key results are available related to pulmonary disease and allergic disorders. Recent studies have also evaluated the relationship of vitamin D to bone health as well as new insights into genetic conditions related to vitamin D metabolism.SummaryRecent studies generally support the recommendations of the Institute of Medicine related to vitamin D intake but there is new and increasing evidence that some health conditions, such as pulmonary diseases in children, might benefit from close monitoring of vitamin D status. However, controlled trials are mostly lacking and there is an inadequate basis from recent studies to recommend high dose vitamin D pending the results of controlled trials. C1 [Abrams, Steven A.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. [Tiosano, Dov] Technion Israel Inst Technol, Bruce Rappaport Fac Med, IL-31096 Haifa, Israel. RP Abrams, SA (reprint author), USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM sabrams@bcm.edu OI Abrams, Steven/0000-0003-4972-9233 FU USDA/ARS [58-6250-6-001] FX This work is a publication of the US Department of Agriculture (USDA)/Agricultural Research Service (ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, and Texas Children's Hospital, Houston, Texas. This project has been funded in part with federal funds from the USDA/ARS under Cooperative Agreement number 58-6250-6-001. Contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. NR 19 TC 1 Z9 1 U1 1 U2 10 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1752-296X EI 1752-2978 J9 CURR OPIN ENDOCRINOL JI Curr. Opin. Endocrinol. Diabetes Obes. PD FEB PY 2014 VL 21 IS 1 BP 51 EP 55 DI 10.1097/01.med.0000436252.53459.ef PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 276IW UT WOS:000328745000010 PM 24300739 ER PT J AU Duarte, ALL Medeiros, RMT Carvalho, FKL Lee, ST Cook, D Pfister, JA Costa, VMM Riet-Correa, F AF Duarte, Amelia L. L. Medeiros, Rosane M. T. Carvalho, Fabricio K. L. Lee, Stephen T. Cook, Daniel Pfister, James A. Costa, Valeria M. M. Riet-Correa, Franklin TI Induction and transfer of resistance to poisoning by Amorimia (Mascagnia) septentrionalis in goats SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE Amorimia septentrionalis; goats; monofluoroacetate; monofluoroacetate degrading bacteria; plant poisoning resistance; sudden death ID PALICOUREA-MARCGRAVII; ACID AB Amorimia septentrionalis contains sodium monofluoroactetate (MFA) and can cause acute heart failure in ruminants when ingested in toxic doses. In this study, we demonstrate that resistance to poisoning by A. septentrionalis can be improved in goats by the repeated administration of non-toxic doses of A. septentrionalis. We also show that increased resistance to poisoning by A. septentrionalis can also be achieved by the transfaunation of ruminal content from goats previously conditioned to be resistant to naive goats. These methods of improving resistance require further study, but appear to provide potential management solutions to mitigate toxicity problems from A. septentrionalis, and perhaps other plant species containing MFA. Copyright (c) 2013 John Wiley & Sons, Ltd. C1 [Duarte, Amelia L. L.; Medeiros, Rosane M. T.; Carvalho, Fabricio K. L.; Costa, Valeria M. M.; Riet-Correa, Franklin] Univ Fed Campina Grande, CSTR, Vet Hosp, Patos de Minas, Paraiba, Brazil. [Lee, Stephen T.; Cook, Daniel; Pfister, James A.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. RP Riet-Correa, F (reprint author), Univ Fed Campina Grande, CSTR, Vet Hosp, BR-58700000 Pb, Paraiba, Brazil. EM franklin.riet@pq.cnpq.br FU National Institute of Science and Technology for Control of Plants Poisoning, CNPq [573534/2008-0] FX This work was funded by the National Institute of Science and Technology for Control of Plants Poisoning, CNPq grant number 573534/2008-0. NR 20 TC 8 Z9 8 U1 0 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD FEB PY 2014 VL 34 IS 2 BP 220 EP 223 DI 10.1002/jat.2860 PG 4 WC Toxicology SC Toxicology GA 274CR UT WOS:000328583200013 PM 23400835 ER PT J AU Brown, G Kelly, M Whitall, D AF Brown, Gregory Kelly, Maggi Whitall, Debra TI Which 'public'? Sampling effects in public participation GIS (PPGIS) and volunteered geographic information (VGI) systems for public lands management SO JOURNAL OF ENVIRONMENTAL PLANNING AND MANAGEMENT LA English DT Article DE public participation; PPGIS; volunteered geographic information; VGI; forest planning; public lands ID NATURAL-RESOURCE MANAGEMENT; PLACE-BASED THEORY; VALUES; INTERNET; FORESTS; TOOL; WEB AB Web 2.0 technologies including Public Participation Geographic Information Systems (PPGIS) and Volunteered Geographic Information (VGI) provide methods for engaging multiple publics in public lands management. We examined the effects of sampling in a PPGIS/VGI application for national forest planning in the US. A random sample (RS) of households and a volunteer public (VP) were invited to participate in an internet-based PPGIS to identify national forest values and use preferences. Spatial and non-spatial group responses were analysed. The VP group expressed stronger utilitarian values and consumptive use preferences while the RS group preferred forest amenities. These results would lead to different planning decisions. PPGIS/VGI methods should include scientific sampling to ground-truth voluntary participation. C1 [Brown, Gregory] Univ Queensland, Environm Planning Dept, Brisbane, Qld 4072, Australia. [Kelly, Maggi] Univ Calif Berkeley, Dept Environm Sci, Ecosyst Sci Div, Berkeley, CA 94720 USA. [Whitall, Debra] US Forest Serv, USDA, Pacific Southwest Reg, Vallejo, CA 94592 USA. RP Brown, G (reprint author), Univ Queensland, Environm Planning Dept, Brisbane, Qld 4072, Australia. EM greg.brown@uq.edu.au OI Kelly, Nina Maggi/0000-0002-0198-2822 NR 45 TC 25 Z9 26 U1 1 U2 50 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 0964-0568 EI 1360-0559 J9 J ENVIRON PLANN MAN JI J. Environ. Plan. Manag. PD FEB 1 PY 2014 VL 57 IS 2 BP 190 EP 214 DI 10.1080/09640568.2012.741045 PG 25 WC Environmental Studies SC Environmental Sciences & Ecology GA 274IR UT WOS:000328600500003 ER PT J AU Ainsworth, EA Serbin, SP Skoneczka, JA Townsend, PA AF Ainsworth, Elizabeth A. Serbin, Shawn P. Skoneczka, Jeffrey A. Townsend, Philip A. TI Using leaf optical properties to detect ozone effects on foliar biochemistry SO PHOTOSYNTHESIS RESEARCH LA English DT Article DE Air pollution; Photochemical reflectance index; Photosynthesis; Remote sensing; Rubisco; Spectroscopy ID RADIATION-USE EFFICIENCY; IN-SEASON BIOMASS; ELEVATED OZONE; SPECTRAL REFLECTANCE; SOYBEAN CULTIVARS; GAS-EXCHANGE; CHLOROPHYLL FLUORESCENCE; TEMPERATURE RESPONSE; VIVO ANALYSIS; PHOTOSYNTHESIS AB Efficient methods for accurate and meaningful high-throughput plant phenotyping are limiting the development and breeding of stress-tolerant crops. A number of emerging techniques, specifically remote sensing methods, have been identified as promising tools for plant phenotyping. These remote sensing methods can be used to accurately and rapidly relate variations in leaf optical properties with important plant characteristics, such as chemistry, morphology, and photosynthetic properties at the leaf and canopy scales. In this study, we explored the potential to utilize optical (lambda = 500-2,400 nm) near-surface remote sensing reflectance spectroscopy to evaluate the effects of ozone pollution on photosynthetic capacity of soybean (Glycine max Merr.). The research was conducted at the Soybean Free Air Concentration Enrichment (SoyFACE) facility where we subjected plants to ambient (44 nL L-1) and elevated ozone (79-82 nL L-1 target) concentrations throughout the growing season. Exposure to elevated ozone resulted in a significant loss of productivity, with the ozone-treated plants displaying a similar to 30 % average decrease in seed yield. From leaf reflectance data, it was also clear that elevated ozone decreased leaf nitrogen and chlorophyll content as well as the photochemical reflectance index (PRI), an optical indicator of the epoxidation state of xanthophyll cycle pigments and thus physiological status. We assessed the potential to use leaf reflectance properties and partial least-squares regression (PLSR) modeling as an alternative, rapid approach to standard gas exchange for the estimation of the maximum rates of RuBP carboxylation (V (c,max)), an important parameter describing plant photosynthetic capacity. While we did not find a significant impact of ozone fumigation on V (c,max), standardized to a reference temperature of 25 A degrees C, the PLSR approach provided accurate and precise estimates of V (c,max) across ambient plots and ozone treatments (r (2) = 0.88 and RMSE = 13.4 mu mol m(-2) s(-1)) based only on the variation in leaf optical properties and despite significant variability in leaf nutritional status. The results of this study illustrate the potential for combining the phenotyping methods used here with high-throughput genotyping methods as a promising approach for elucidating the basis for ozone tolerance in sensitive crops. C1 [Ainsworth, Elizabeth A.; Skoneczka, Jeffrey A.] Univ Illinois, ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA. [Ainsworth, Elizabeth A.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Serbin, Shawn P.; Townsend, Philip A.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Russell Labs 226, Madison, WI 53706 USA. RP Ainsworth, EA (reprint author), Univ Illinois, ARS, Global Change & Photosynth Res Unit, USDA, 1201 W Gregory Dr, Urbana, IL 61801 USA. EM lisa.ainsworth@ars.usda.gov RI Serbin, Shawn/B-6392-2009; Townsend, Philip/B-5741-2008 OI Serbin, Shawn/0000-0003-4136-8971; Townsend, Philip/0000-0001-7003-8774 FU Agriculture and Food Research Initiative, USDA National Institute of Food and Agriculture [2010-65114-20355]; NASA Earth and Space Science Fellowship [NNX08AV07H]; NASA HyspIRI Preparatory Grant [NNX10AJ94G] FX We thank Chris Montes, Kannan Puthuval, and David Drag for managing SoyFACE, and Donald Ort for facilitating research. Chris Montes, Joe Sullivan, and Amy Betzelberger assisted with measurements of leaf optical properties and gas exchange. This study was supported in part by the Agriculture and Food Research Initiative competitive grant no. 2010-65114-20355 from the USDA National Institute of Food and Agriculture to E. A. A, NASA Earth and Space Science Fellowship NNX08AV07H to S.P.S. and NASA HyspIRI Preparatory Grant NNX10AJ94G to P.A.T. NR 80 TC 14 Z9 15 U1 7 U2 96 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0166-8595 EI 1573-5079 J9 PHOTOSYNTH RES JI Photosynth. Res. PD FEB PY 2014 VL 119 IS 1-2 BP 65 EP 76 DI 10.1007/s11120-013-9837-y PG 12 WC Plant Sciences SC Plant Sciences GA 277VS UT WOS:000328848000006 PM 23657827 ER PT J AU Hwang, S Ray, JD Cregan, PB King, CA Davies, MK Purcell, LC AF Hwang, Sadal Ray, Jeffery D. Cregan, Perry B. King, C. Andy Davies, Marilynn K. Purcell, Larry C. TI Genetics and mapping of quantitative traits for nodule number, weight, and size in soybean (Glycine max L.[Merr.]) SO EUPHYTICA LA English DT Article DE Nitrogen fixation; Nodule; QTL; Soybean; Symbiosis ID SYMBIOTIC NITROGEN-FIXATION; L. MERR.; EXPERIMENTAL POPULATIONS; GENOTYPIC DIFFERENCES; RECEPTOR KINASE; COMPLEX TRAITS; SEED PROTEIN; LOCI; NODULATION; YIELD AB Soybean research has found that nodule traits, especially nodule biomass, are associated with N-2 fixation ability. Two genotypes, differing in nodule number per plant and individual nodule weight, KS4895 and Jackson, were mated to create 17 F-3- and 80 F-5-derived RILs. The population was mapped with 664 informative markers with an average distance of less than 20 cM between adjacent markers. Nodule traits were evaluated in 3-year field trials. Broad-sense heritability for nodule number (no. plant(-1)), individual nodule dry weight (mg nodule(-1)), individual nodule size (mm nodule(-1)), and total nodule dry weight (g plant(-1)) was 0.41, 0.42, 0.45, and 0.27, respectively. Nodule number was negatively correlated with individual nodule weight and size. Nodule number, individual nodule weight, and size are major components which likely contributed to increased total nodule weight per plant. Composite interval mapping (CIM) identified eight QTLs for nodule number with R-2 values ranging from 0.14 to 0.20. Multiple interval mapping (MIM) identified two QTLs for nodule number, one of which was located close to the QTL identified with CIM. Six QTLs for individual nodule weight were detected with CIM, and one QTL was identified with MIM. For nodule size, CIM identified seven QTLs with R-2 values ranging from 0.14 to 0.27. Five QTLs for total nodule weight were detected with CIM, one of which was located close to a QTL identified with MIM. These results document the first QTL information on nodule traits in soybean from field experiments utilizing a dense, complete linkage map. C1 [Hwang, Sadal; King, C. Andy; Davies, Marilynn K.; Purcell, Larry C.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. [Ray, Jeffery D.] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, BARC West, Beltsville, MD 20705 USA. RP Purcell, LC (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. EM lpurcell@uark.edu FU United Soybean Board; United States Department of Agriculture-Agriculture Research Service (USDA-ARS) [6402-21220-012-00D] FX The authors gratefully acknowledge partial financial support for this research from the United Soybean Board and from the United States Department of Agriculture-Agriculture Research Service (USDA-ARS) project number 6402-21220-012-00D. Editorial assistance from Penny McGee is greatly appreciated. NR 69 TC 7 Z9 8 U1 2 U2 43 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD FEB PY 2014 VL 195 IS 3 BP 419 EP 434 DI 10.1007/s10681-013-1005-0 PG 16 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 270LG UT WOS:000328319300009 ER PT J AU Campbell, BT Greene, JK Wu, J Jones, DC AF Campbell, B. T. Greene, J. K. Wu, J. Jones, D. C. TI Assessing the breeding potential of day-neutral converted racestock germplasm in the Pee Dee cotton germplasm enhancement program SO EUPHYTICA LA English DT Article DE Cotton; Exotic germplasm; Breeding potential; Combining ability ID ACCESSION DERIVED GERMPLASM; UPLAND COTTON; VARIANCE-COMPONENTS; FIBER TRAITS; GENETIC VARIANCES; CULTIVAR CROSSES; LINES; IMPROVEMENT; REGISTRATION; YIELD AB The primitive, upland cotton landrace collection represents one of the untapped genetic resources in cotton breeding programs. Efforts to utilize these resources have been slow, but the development of day-neutral converted germplasm lines offers tremendous potential for broadening the genetic base in upland cotton. Using topcross hybrids involving elite germplasm from the unique Pee Dee germplasm enhancement program, we evaluated the breeding potential of a select number of day-neutral converted racestocks. The mean performance of parental lines and F-2 topcross hybrids along with genetic effect estimates indicate that day-neutral converted germplasm lines decreased agronomic performance while increasing fiber quality performance. Results suggest that crosses between day-neutral converted racestocks and elite Pee Dee germplasm lines result in new allelic combinations associated with improved fiber quality performance that interact in a non-additive way. However, it appears that converted racestocks transmit negatively correlated alleles for agronomic performance and fiber quality. These negatively correlated allelic combinations present a major challenge for cotton breeding programs. Future efforts that incorporate DNA based selection methods to identify and fix introgressed segments from converted racestocks and their offspring should enhance the use of the genetic variation present in the primitive racestock germplasm accessions. C1 [Campbell, B. T.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Greene, J. K.] Clemson Univ, Blackville, SC 29817 USA. [Wu, J.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Jones, D. C.] Cotton Inc, Cary, NC 27513 USA. RP Campbell, BT (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. EM todd.campbell@ars.usda.gov FU U.S. Department of Agriculture [6657-21000-006-00D]; Cotton Incorporated FX This research project was supported by funding from CRIS No. 6657-21000-006-00D of the U.S. Department of Agriculture and a grant from Cotton Incorporated. Special thanks to Bobby Fisher, Dan Robinson, and summer students for technical assistance. Special thanks also to Dr. Jack McCarty for providing seed of day-neutral converted racestocsks. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. NR 41 TC 3 Z9 3 U1 0 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD FEB PY 2014 VL 195 IS 3 BP 453 EP 465 DI 10.1007/s10681-013-1007-y PG 13 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 270LG UT WOS:000328319300011 ER PT J AU Ray, CD Ma, L Wilson, T Wilson, D McCreery, L Wiedenbeck, JK AF Ray, Charles D. Ma, Li Wilson, Thomas Wilson, Daniel McCreery, Lew Wiedenbeck, Janice K. TI Biomass boiler conversion potential in the eastern United States SO RENEWABLE ENERGY LA English DT Article DE Biomass; Conversion; Industrial boilers; Commercial boilers AB The U.S. is the world's leading consumer of primary energy. A large fraction of this energy is used in boiler installations to generate steam and hot water for heating applications. It is estimated there are total 163,000 industrial and commercial boilers in use in the United States of all sizes. This paper characterizes the commercial and industrial boilers in the 37 states of the Midwest, Northeast, and Southern regions of the U.S. in term of number of units, unit capacity, aggregate capacity, and fuel type. A methodology is developed for evaluating and ranking the potential for converting from existing fossil-fuel boilers to biomass boilers in these states. In total, 3495 oil and coal boiler units in industrial and commercial buildings, and 1067 major wood energy facilities in the 37 eastern states were identified. These represent a subset of existing and potential conversions from fossil fuels to woody biomass. Based on this sample and energy consumption data from the Energy Information Administration (EIA), we estimate that there are currently 31,776 oil, coal, and propane boiler units over 0.5 MMBtus/hour capacity in these 37 states, representing a total energy consumption of 1.7 quadrillion Btus, or roughly the equivalent of 287 million barrels of oil. Were these units all converted to woody biomass fuel, they would consume a total of 121 million dry tons of wood per year, about three times the most recent US DOE estimates of woody biomass availability in those regions. Since only the most economical conversions typically occur, the reality of woody biomass market availability combined with thermal fossil-fuel consumption patterns suggests that roughly one-third of all potential projects could be achieved under sustainable utilization of existing biomass feedstocks in the three regions. Analysis of the results indicates that a targeted response to wood-conversion initiatives will yield the most successful program of fossil-fuel replacement in thermal applications. A ranking index developed in this study through analysis of existing boiler installations and availability of wood feedstocks suggests that the top ten states in the eastern United States on which to focus future messaging, feasibility studies, and policy development for potential woody biomass conversions are: 1. Maine, 2. Texas, 3. New York, 4. Florida, 5. Georgia, 6. Alabama, 7. South Carolina, 8. North Carolina, 9. Arkansas, 10. Pennsylvania. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Ray, Charles D.; Ma, Li] Penn State Univ, University Pk, PA 16802 USA. [Wilson, Thomas; Wilson, Daniel] Wilson Engn Serv, Meadville, PA 16335 USA. [McCreery, Lew] US Forest Serv, Northeastern Area, Morgantown, WV 26501 USA. [Wiedenbeck, Janice K.] US Forest Serv, No Res Stn, Princeton, WV 24740 USA. RP Ray, CD (reprint author), Penn State Univ, Room 205 Forest Resources Bldg, University Pk, PA 16802 USA. EM cdrpsu@gmail.com; mali67988@gmail.com; TWilson@wilsonengineeringservices.com; dwilson@wilsonengineeringservices.com; lmccreery@fs.fed.us; jwiedenbeck@fs.fed.us NR 7 TC 6 Z9 6 U1 3 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-1481 J9 RENEW ENERG JI Renew. Energy PD FEB PY 2014 VL 62 BP 439 EP 453 DI 10.1016/j.renene.2013.07.019 PG 15 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA 267JX UT WOS:000328095000050 ER PT J AU Fang, T Huang, LH AF Fang, Ting Huang, Lihan TI Growth and survival kinetics of Listeria monocytogenes in cooked egg whites SO FOOD CONTROL LA English DT Article DE Hard-boiled eggs; Listeria monocytogenes; Microbial safety; Predictive modeling ID ANTIBACTERIAL ACTIVITY; LYSOZYME; MODEL; SPOILAGE AB Peeled hard-boiled eggs (HBE) are ready-to-eat products susceptible to surface contamination by Listeria monocytogenes. This study investigated the growth and survival of L monocytogenes between 4 and 43 C in egg whites cooked under different conditions (70 degrees C for 15 min, 80 degrees C for 20 min, and 100 degrees C for 10 min). L monocytogenes inoculated to samples cooked at 100 degrees C could grow uninhibitedly between 4 and 40 degrees C, exhibiting no lag phases, but failed to grow at 43 degrees C. The growth process was described by a 3-parameter logistic primary model, with the specific growth rates fitted equally well to the Ratkowsky square-root and Cardinal models. According to the Ratkowsky square-root model, the estimated minimum (nominal) and maximum growth temperatures were -03 and 47 degrees C, which were 1.6 and 44.3 degrees C, respectively, according to the Cardinal model. L monocytogenes did not grow well when inoculated to egg white samples cooked at 70 and 80 degrees C. Images of scanning electron microscopy showed that L monocytogenes was damaged in samples cooked at these temperatures. Although experiencing a <2 log cfu/g initial growth, L monocytogenes was inhibited in these samples at all storage temperatures, probably due to the antimicrobial activities of heat-denatured and polymerized lysozyme formed at 70 and 80 degrees C, which were absent in samples cooked at 100 degrees C. The results of this study showed that cooking temperature affected the survival and growth of L monocytogenes in cooked egg whites, suggesting that HBEs may be cooked at a lower temperature in order to retain the antilisterial activities. The mathematical models developed in this study can be used to predict the growth and survival of L monocytogenes in BHEs and for conducting risk analysis of this type of products. Published by Elsevier Ltd. C1 [Fang, Ting] Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350001, Peoples R China. [Huang, Lihan] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Huang, LH (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov NR 22 TC 3 Z9 4 U1 1 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD FEB PY 2014 VL 36 IS 1 BP 191 EP 198 DI 10.1016/j.foodcont.2013.08.034 PG 8 WC Food Science & Technology SC Food Science & Technology GA 257DJ UT WOS:000327363500029 ER PT J AU Dhakal, S Li, YY Peng, YK Chao, KL Qin, JW Guo, LH AF Dhakal, Sagar Li, Yongyu Peng, Yankun Chao, Kuanglin Qin, Jianwei Guo, Langhua TI Prototype instrument development for non-destructive detection of pesticide residue in apple surface using Raman technology SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Raman spectroscopy; 785 nm Laser; Pesticide; Apple; Optical instrument prototype ID FRUITS; CHROMATOGRAPHY; SAMPLES; DECAY AB Apple is one of the highly consumed fruit and also a major source of pesticide carrier to human health. This study explores the application of Raman spectroscopy for detection of commercially available organophosphorus (chlorpyrifos) pesticide in apple surface. Optical instrument prototype equipped with Raman spectroscopy system with 785 nm laser excitation source was developed for non-destructive, rapid and accurate detection of pesticide residue in apple surface, overcoming the loopholes of traditional detection methods. Software was self developed to control the functionality of Raman CCD, acquire and process the Raman spectral data and display result in real time. The samples detected by the developed system were tested in High Performance Liquid Chromatography. The result shows that the developed system can detect chlorpyrifos residue to minimum limit of 6.69 mg/kg in apple surface within less than 4 s. This innovative and promising system can be a breakthrough technology for pesticide detection in fruits and vegetables. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Dhakal, Sagar; Li, Yongyu; Peng, Yankun; Guo, Langhua] China Agr Univ, Natl R&D Ctr Agroproc Equipments, Beijing 100083, Peoples R China. [Chao, Kuanglin; Qin, Jianwei] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. RP Peng, YK (reprint author), China Agr Univ, Natl R&D Ctr Agroproc Equipments, Beijing 100083, Peoples R China. EM ypeng@cau.edu.cn FU Special Fund for Agro-scientific Research in the Public Interest [201003008]; Recommend international advanced agricultural science and technology plan [2012-Z17]; Technical assistance Projects in developing countries, China [I20130009] FX The authors wish to thank the Special Fund for Agro-scientific Research in the Public Interest (Project No. 201003008), Recommend international advanced agricultural science and technology plan (Project No. 2012-Z17), and Technical assistance Projects in developing countries (Project No. I20130009), China, for providing funding support for this research. NR 24 TC 27 Z9 31 U1 4 U2 82 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD FEB PY 2014 VL 123 BP 94 EP 103 DI 10.1016/j.jfoodeng.2013.09.025 PG 10 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 256ET UT WOS:000327293200012 ER PT J AU Malone, RW Nolan, BT Ma, L Kanwar, RS Pederson, C Heilman, P AF Malone, R. W. Nolan, B. T. Ma, L. Kanwar, R. S. Pederson, C. Heilman, P. TI Effects of tillage and application rate on atrazine transport to subsurface drainage: Evaluation of RZWQM using a six-year field study SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Pesticide transport; Macroporosity; Preferential flow; Artificial subsurface drainage; Modeling; Management effects; Water quality ID WATER-QUALITY MODEL; SENSITIVITY-ANALYSIS; PESTICIDE TRANSPORT; UNITED-STATES; REGRESSION-MODELS; GROUND-WATER; CROP YIELD; SOIL; FLOW; CALIBRATION AB Well tested agricultural system models can improve our understanding of the water quality effects of management practices under different conditions. The Root Zone Water Quality Model (RZWQM) has been tested under a variety of conditions. However, the current model's ability to simulate pesticide transport to subsurface drain flow over a long term period under different tillage systems and application rates is not clear. Therefore, we calibrated and tested RZWQM using six years of data from Nashua, Iowa. In this experiment, atrazine was spring applied at 2.8 (1990-1992) and 0.6 kg/ha/yr (1993-1995) to two 0.4 ha plots with different tillage (till and no-till). The observed and simulated average annual flow weighted atrazine concentrations (FWAC) in subsurface drain flow from the no-till plot were 3.7 and 3.2 mu g/L, respectively for the period with high atrazine application rates, and 0.8 and 0.9 mu g/L, respectively for the period with low application rates. The 1990-1992 observed average annual FWAC difference between the no-till and tilled plot was 2.4 mu g/L while the simulated difference was 2.1 mu g/L. These observed and simulated differences for 1993-1995 were 0.1 and 0.1 mu g/L, respectively. The Nash-Sutcliffe model performance statistic (EF) for cumulative atrazine flux to subsurface drain flow was 0.93 for the no-till plot testing years (1993-1995), which is comparable to other recent model tests. The value of EF is 1.0 when simulated data perfectly match observed data. The order of selected parameter sensitivity for RZWQM simulated FWAC was atrazine partition coefficient > number of macropores > atrazine half life in soil > soil hydraulic conductivity. Simulations from 1990 to 1995 with four different atrazine application rates applied at a constant rate throughout the simulation period showed concentrations in drain flow for the no-till plot to be twice those of the tilled plot. The differences were more pronounced in the early simulation period (1990-1992), partly because of the characteristics of macropore flow during large storms. The results suggest that RZWQM is a promising tool to study pesticide transport to subsurface drain flow under different tillage systems and application rates over several years, the concentrations of atrazine in drain flow can be higher with no-till than tilled soil over a range of atrazine application rates, and atrazine concentrations in drain flow are sensitive to the macropore flow characteristics under different tillage systems and rainfall timing and intensity. Published by Elsevier B.V. C1 [Malone, R. W.] USDA ARS, Ames, IA USA. [Nolan, B. T.] US Geol Survey, Reston, VA 22092 USA. [Ma, L.] USDA ARS, Ft Collins, CO 80522 USA. [Kanwar, R. S.; Pederson, C.] Iowa State Univ, Ames, IA USA. [Heilman, P.] USDA ARS, Tucson, AZ USA. RP Malone, RW (reprint author), USDA ARS, Ames, IA USA. EM rob.malone@ars.usda.gov NR 55 TC 6 Z9 7 U1 3 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JAN 31 PY 2014 VL 132 BP 10 EP 22 DI 10.1016/j.agwat.2013.09.009 PG 13 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 275XH UT WOS:000328712200002 ER PT J AU Elsik, CG Worley, KC Bennett, AK Beye, M Camara, F Childers, CP de Graaf, DC Debyser, G Deng, JX Devreese, B Elhaik, E Evans, JD Foster, LJ Graur, D Guigo, R Hoff, KJ Holder, ME Hudson, ME Hunt, GJ Jiang, HY Joshi, V Khetani, RS Kosarev, P Kovar, CL Ma, J Maleszka, R Moritz, RFA Munoz-Torres, MC Murphy, TD Muzny, DM Newsham, IF Reese, JT Robertson, HM Robinson, GE Rueppell, O Solovyev, V Stanke, M Stolle, E Tsuruda, JM Van Vaerenbergh, M Waterhouse, RM Weaver, DB Whitfield, CW Wu, YQ Zdobnov, EM Zhang, L Zhu, DH Gibbs, RA AF Elsik, Christine G. Worley, Kim C. Bennett, Anna K. Beye, Martin Camara, Francisco Childers, Christopher P. de Graaf, Dirk C. Debyser, Griet Deng, Jixin Devreese, Bart Elhaik, Eran Evans, Jay D. Foster, Leonard J. Graur, Dan Guigo, Roderic Hoff, Katharina Jasmin Holder, Michael E. Hudson, Matthew E. Hunt, Greg J. Jiang, Huaiyang Joshi, Vandita Khetani, Radhika S. Kosarev, Peter Kovar, Christie L. Ma, Jian Maleszka, Ryszard Moritz, Robin F. A. Munoz-Torres, Monica C. Murphy, Terence D. Muzny, Donna M. Newsham, Irene F. Reese, Justin T. Robertson, Hugh M. Robinson, Gene E. Rueppell, Olav Solovyev, Victor Stanke, Mario Stolle, Eckart Tsuruda, Jennifer M. Van Vaerenbergh, Matthias Waterhouse, Robert M. Weaver, Daniel B. Whitfield, Charles W. Wu, Yuanqing Zdobnov, Evgeny M. Zhang, Lan Zhu, Dianhui Gibbs, Richard A. CA HGSC Prod Teams Honey Bee Genome Sequencing Consor TI Finding the missing honey bee genes: lessons learned from a genome upgrade SO BMC GENOMICS LA English DT Article DE Apis mellifera; GC content; Gene annotation; Gene prediction; Genome assembly; Genome improvement; Genome sequencing; Repetitive DNA; Transcriptome ID OPEN READING FRAMES; APIS-MELLIFERA; TRANSPOSABLE ELEMENTS; CLASSIFICATION-SYSTEM; PROTEIN FAMILIES; DOMAIN DATABASE; TANDEM REPEATS; DNA-SEQUENCES; SOCIAL INSECT; DRAFT GENOME AB Background: The first generation of genome sequence assemblies and annotations have had a significant impact upon our understanding of the biology of the sequenced species, the phylogenetic relationships among species, the study of populations within and across species, and have informed the biology of humans. As only a few Metazoan genomes are approaching finished quality (human, mouse, fly and worm), there is room for improvement of most genome assemblies. The honey bee (Apis mellifera) genome, published in 2006, was noted for its bimodal GC content distribution that affected the quality of the assembly in some regions and for fewer genes in the initial gene set (OGSv1.0) compared to what would be expected based on other sequenced insect genomes. Results: Here, we report an improved honey bee genome assembly (Amel_4.5) with a new gene annotation set (OGSv3.2), and show that the honey bee genome contains a number of genes similar to that of other insect genomes, contrary to what was suggested in OGSv1.0. The new genome assembly is more contiguous and complete and the new gene set includes similar to 5000 more protein-coding genes, 50% more than previously reported. About 1/6 of the additional genes were due to improvements to the assembly, and the remaining were inferred based on new RNAseq and protein data. Conclusions: Lessons learned from this genome upgrade have important implications for future genome sequencing projects. Furthermore, the improvements significantly enhance genomic resources for the honey bee, a key model for social behavior and essential to global ecology through pollination. C1 [Elsik, Christine G.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [Elsik, Christine G.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Elsik, Christine G.] Univ Missouri, MU Informat Inst, Columbia, MO 65211 USA. [Elsik, Christine G.; Bennett, Anna K.; Childers, Christopher P.; Munoz-Torres, Monica C.; Reese, Justin T.] Georgetown Univ, Dept Biol, Washington, DC 20057 USA. [Worley, Kim C.; Deng, Jixin; Holder, Michael E.; Joshi, Vandita; Kovar, Christie L.; Muzny, Donna M.; Newsham, Irene F.; Wu, Yuanqing; Zhang, Lan; Zhu, Dianhui; Gibbs, Richard A.; HGSC Prod Teams] Baylor Coll Med, Human Genome Sequencing Ctr, Dept Mol & Human Genet, Houston, TX 77030 USA. [Beye, Martin] Univ Dusseldorf, Inst Evolutionary Genet, D-40225 Dusseldorf, Germany. [Camara, Francisco; Guigo, Roderic] Univ Pompeu Fabra, Ctr Genom Regulat, E-08003 Barcelona, Catalonia, Spain. [Childers, Christopher P.; Reese, Justin T.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [de Graaf, Dirk C.; Van Vaerenbergh, Matthias] Univ Ghent, Lab Zoophysiol, B-9000 Ghent, Belgium. [Debyser, Griet; Devreese, Bart] Univ Ghent, Lab Prot Biochem & Biomol Engn, B-9000 Ghent, Belgium. [Elhaik, Eran] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Mental Hlth, Baltimore, MD 21205 USA. [Evans, Jay D.] USDA ARS, BARC E, Bee Res Lab, Beltsville, MD 20705 USA. [Foster, Leonard J.] Univ British Columbia, Ctr High Throughput Biol, Dept Biochem & Mol Biol, Vancouver, BC V5Z 1M9, Canada. [Graur, Dan] Univ Houston, Dept Biol & Biochem, Houston, TX 77204 USA. [Hoff, Katharina Jasmin; Stanke, Mario] Ernst Moritz Arndt Univ Greifswald, Inst Math & Comp Sci, D-17487 Greifswald, Germany. [Hudson, Matthew E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Hudson, Matthew E.; Ma, Jian] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Hunt, Greg J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Jiang, Huaiyang] Univ Pittsburgh, Dept Obstet Gynecol & Reprod Sci, Pittsburgh, PA 15260 USA. [Khetani, Radhika S.] Univ Illinois, Roy J Carver Biotechnol Ctr, High Performance Biol Comp HPCBio, Urbana, IL 61801 USA. [Kosarev, Peter] Softberry Inc, Mt Kisco, NY 10549 USA. [Ma, Jian] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA. [Maleszka, Ryszard] Australian Natl Univ, Res Sch Biol, Canberra, ACT 0200, Australia. [Moritz, Robin F. A.; Stolle, Eckart] Univ Halle Wittenberg, Inst Zool, D-06099 Halle, Saale, Germany. [Munoz-Torres, Monica C.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Genom Div, Berkeley, CA 94720 USA. [Murphy, Terence D.] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. [Robertson, Hugh M.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Robinson, Gene E.] Univ Illinois, Inst Genom Biol, Dept Entomol, Neurosci Program, Urbana, IL 61801 USA. [Rueppell, Olav] Univ N Carolina, Dept Biol, Greensboro, NC 27412 USA. [Solovyev, Victor] King Abdullah Univ Sci & Technol, Comp Elect & Math Sci & Engn Div, Thuwal 239556900, Saudi Arabia. [Tsuruda, Jennifer M.] Clemson Univ, Clemson, SC 29634 USA. [Waterhouse, Robert M.; Zdobnov, Evgeny M.] Univ Geneva, CH-1211 Geneva, Switzerland. [Waterhouse, Robert M.; Zdobnov, Evgeny M.] Swiss Inst Bioinformat, CMU, CH-1211 Geneva, Switzerland. [Weaver, Daniel B.] Genformatic, Austin, TX 78731 USA. [Whitfield, Charles W.] Univ Illinois, Dept Entomol, Neurosci Program, Program Ecol & Evolutionary Biol, Urbana, IL 61801 USA. RP Elsik, CG (reprint author), Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. EM elsikc@missouri.edu; kworley@bcm.edu RI Stolle, Eckart/G-3780-2011; Moritz, Robin/K-6053-2012; Rueppell, Olav/G-2679-2010; Hudson, Matthew/A-4438-2008; Evans, Jay/C-8408-2012; Devreese, Bart/B-2011-2009; Camara Ferreira, Francisco/G-9841-2015; Guigo, Roderic/D-1303-2010; Waterhouse, Robert/A-1858-2010; Maleszka, Ryszard/A-6078-2008; Zdobnov, Evgeny/K-1133-2012; Elsik, Christine/C-4120-2017; OI Stolle, Eckart/0000-0001-7638-4061; Moritz, Robin/0000-0003-0791-887X; Rueppell, Olav/0000-0001-5370-4229; Hudson, Matthew/0000-0002-4737-0936; Evans, Jay/0000-0002-0036-4651; Devreese, Bart/0000-0002-9764-2581; Camara Ferreira, Francisco/0000-0002-1971-5466; Guigo, Roderic/0000-0002-5738-4477; Waterhouse, Robert/0000-0003-4199-9052; Maleszka, Ryszard/0000-0003-1855-555X; Elsik, Christine/0000-0002-4248-7713; Childers, Anna/0000-0002-0747-8539; Childers, Chris/0000-0002-1253-5550 FU National Human Genome Research Institute, National Institutes of Health (NHGRI, NIH) [U54 HG003273]; USDA National Institute of Food Agriculture [2010-65205-20407]; Clare Luce Booth Fellowship at Georgetown University FX Funding for the project was provided by a grant to RG from the National Human Genome Research Institute, National Institutes of Health (NHGRI, NIH) U54 HG003273. Contributions from members of the CGE lab were supported by Agriculture and Food Research Initiative Competitive grant no. 2010-65205-20407 from the USDA National Institute of Food Agriculture. AKB was supported by a Clare Luce Booth Fellowship at Georgetown University. The authors are grateful for the HGSC sequence production teams (Patil, S., Gubbala, S., Aqrawi, P., Arias, F., Bess, C., Blankenburg, K. B., Brocchini, M., Buhay, C., Challis, D., Chang, K., Chen, D., Coleman, P., Drummond, J., English, A., Evani, U., Francisco, L., Fu, Q., Goodspeed, R., Haessly, T. H., Hale, W., Han, H., Holder, M., Hu, Y., Jackson, L., Jakkamsetti, A., Jayaseelan, J. C., Kakkar, N., Kalra, D., Kandadi, H., Lee, S., Li, H., Liu, Y., Macmil, S., Mandapat, C. M., Mata, R., Mathew, T., Matskevitch, T., Munidasa, M., Nagaswamy, U., Najjar, R., Nguyen, N., Niu, J., Opheim, D., Palculict, T., Paul, S., Pellon, M., Perales, L., Pham, C., Pham, P., Pu, L.-L., Qi, S., Qu, J., Ren, Y., Ruth, R.T., Saada, N., Sabo, A., San Lucas, F., Sershen, C., Shafer, J., Shah, N., Shelton, R., Song, X.-Z., Tabassum, N., Tang, L., Taylor, A., Taylor, M., Velamala, V., Wan, Z., Wang, L., Wang, Y., Warren, J., Weissenberger, G., Wilczek-Boney, K. B., Yao, J., Yin, B., Yu, J., Zhang, J., Zhang, L., Zhou, C., Zhu, D., Zhu, Y., and Zou, X.), and the input of other members of the HGSC genome assembly team. NR 108 TC 86 Z9 87 U1 4 U2 73 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JAN 30 PY 2014 VL 15 AR 86 DI 10.1186/1471-2164-15-86 PG 29 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AA5DT UT WOS:000331116900002 PM 24479613 ER PT J AU Caniato, FF Hamblin, MT Guimaraes, CT Zhang, ZW Schaffert, RE Kochian, LV Magalhaes, JV AF Caniato, Fernanda F. Hamblin, Martha T. Guimaraes, Claudia T. Zhang, Zhiwu Schaffert, Robert E. Kochian, Leon V. Magalhaes, Jurandir V. TI Association Mapping Provides Insights into the Origin and the Fine Structure of the Sorghum Aluminum Tolerance Locus, Alt(SB) SO PLOS ONE LA English DT Article ID MULTILOCUS GENOTYPE DATA; GENOME-WIDE ASSOCIATION; LINKAGE DISEQUILIBRIUM; ACID SOILS; GENE-EXPRESSION; ACTIVATED CITRATE; WEST-AFRICA; POPULATION; TRANSPORTER; MAIZE AB Root damage caused by aluminum (Al) toxicity is a major cause of grain yield reduction on acid soils, which are prevalent in tropical and subtropical regions of the world where food security is most tenuous. In sorghum, Al tolerance is conferred by SbMATE, an Al-activated root citrate efflux transporter that underlies the major Al tolerance locus, Alt(SB), on sorghum chromosome 3. We used association mapping to gain insights into the origin and evolution of Al tolerance in sorghum and to detect functional variants amenable to allele mining applications. Linkage disequilibrium across the Alt(SB) locus decreased much faster than in previous reports in sorghum, and reached basal levels at approximately 1000 bp. Accordingly, intra-locus recombination events were found to be extensive. SNPs and indels highly associated with Al tolerance showed a narrow frequency range, between 0.06 and 0.1, suggesting a rather recent origin of Al tolerance mutations within Alt(SB). A haplotype network analysis suggested a single geographic and racial origin of causative mutations in primordial guinea domesticates in West Africa. Al tolerance assessment in accessions harboring recombinant haplotypes suggests that causative polymorphisms are localized to a similar to 6 kb region including intronic polymorphisms and a transposon (MITE) insertion, whose size variation has been shown to be positively correlated with Al tolerance. The SNP with the strongest association signal, located in the second SbMATE intron, recovers 9 of the 14 highly Al tolerant accessions and 80% of all the Al tolerant and intermediately tolerant accessions in the association panel. Our results also demonstrate the pivotal importance of knowledge on the origin and evolution of Al tolerance mutations in molecular breeding applications. Allele mining strategies based on associated loci are expected to lead to the efficient identification, in diverse sorghum germplasm, of Al tolerant accessions able maintain grain yields under Al toxicity. C1 [Caniato, Fernanda F.; Guimaraes, Claudia T.; Schaffert, Robert E.; Magalhaes, Jurandir V.] Embrapa Maize & Sorghum, Sete Lagoas, MG, Brazil. [Hamblin, Martha T.; Zhang, Zhiwu] Cornell Univ, Inst Genom Divers, Ithaca, NY USA. [Kochian, Leon V.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Magalhaes, JV (reprint author), Embrapa Maize & Sorghum, Sete Lagoas, MG, Brazil. EM jurandir.magalhaes@embrapa.br OI Kochian, Leon/0000-0003-3416-089X; Zhang, Zhiwu/0000-0002-5784-9684 FU CGIAR Generation Challenge Program [G3007.04]; Embrapa Macroprogram; McKnight Foundation Collaborative Crop Research Program; Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG); National Council for Scientific and Technological Development (CNPq) FX The authors acknowledge funding from the CGIAR Generation Challenge Program (project G3007.04), the Embrapa Macroprogram and the McKnight Foundation Collaborative Crop Research Program. The authors are also thankful to the Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) for a post-doctoral fellowship granted to FFC and to The National Council for Scientific and Technological Development (CNPq) for support to JVM and CTG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 71 TC 11 Z9 11 U1 3 U2 48 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 30 PY 2014 VL 9 IS 1 AR e87438 DI 10.1371/journal.pone.0087438 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 302PJ UT WOS:000330617100085 PM 24498106 ER PT J AU van Alphen, LB Wenzel, CQ Richards, MR Fodor, C Ashmus, RA Stahl, M Karlyshev, AV Wren, BW Stintzi, A Miller, WG Lowary, TL Szymanski, CM AF van Alphen, Lieke B. Wenzel, Cory Q. Richards, Michele R. Fodor, Christopher Ashmus, Roger A. Stahl, Martin Karlyshev, Andrey V. Wren, Brendan W. Stintzi, Alain Miller, William G. Lowary, Todd L. Szymanski, Christine M. TI Biological Roles of the O-Methyl Phosphoramidate Capsule Modification in Campylobacter jejuni SO PLOS ONE LA English DT Article ID SP-NOV.; GASTROINTESTINAL-TRACT; UNITED-STATES; POLYSACCHARIDE; INFECTION; HUMANS; SEQUENCE; PATHOGEN; LIPOOLIGOSACCHARIDE; LIPOPOLYSACCHARIDES AB Campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, and the capsular polysaccharide (CPS) of this organism is required for persistence and disease. C. jejuni produces over 47 different capsular structures, including a unique O-methyl phosphoramidate (MeOPN) modification present on most C. jejuni isolates. Although the MeOPN structure is rare in nature it has structural similarity to some synthetic pesticides. In this study, we have demonstrated, by whole genome comparisons and high resolution magic angle spinning NMR, that MeOPN modifications are common to several Campylobacter species. Using MeOPN biosynthesis and transferase mutants generated in C. jejuni strain 81-176, we observed that loss of MeOPN from the cell surface correlated with increased invasion of Caco-2 epithelial cells and reduced resistance to killing by human serum. In C. jejuni, the observed serum mediated killing was determined to result primarily from activation of the classical complement pathway. The C. jejuni MeOPN transferase mutant showed similar levels of colonization relative to the wild-type in chickens, but showed a five-fold drop in colonization when co-infected with the wild-type in piglets. In Galleria mellonella waxmoth larvae, the MeOPN transferase mutant was able to kill the insects at wildtype levels. Furthermore, injection of the larvae with MeOPN-linked monosaccharides or CPS purified from the wild-type strain did not result in larval killing, indicating that MeOPN does not have inherent insecticidal activity. C1 [van Alphen, Lieke B.; Wenzel, Cory Q.; Richards, Michele R.; Fodor, Christopher; Szymanski, Christine M.] Univ Alberta, Dept Biol Sci, Alberta Glyc Ctr, Edmonton, AB, Canada. [Ashmus, Roger A.; Lowary, Todd L.] Univ Alberta, Dept Chem, Alberta Glyc Ctr, Edmonton, AB, Canada. [Stahl, Martin; Stintzi, Alain] Univ Ottawa, Ottawa Inst Syst Biol, Dept Biochem Microbiol & Immunol, Ottawa, ON, Canada. [Karlyshev, Andrey V.] Univ Kingston, Sch Life Sci, London, England. [Wren, Brendan W.] London Sch Hyg & Trop Med, London WC1, England. [Miller, William G.] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA USA. RP Szymanski, CM (reprint author), Univ Alberta, Dept Biol Sci, Alberta Glyc Ctr, Edmonton, AB, Canada. EM cszymans@ualberta.ca RI Richards, Mickey/E-8140-2011; Karlyshev, Andrey/D-2797-2011; OI Richards, Mickey/0000-0002-1973-5678; Karlyshev, Andrey/0000-0003-3124-019X; Lowary, Todd/0000-0002-8331-8211 FU Alberta Innovates Health Solutions Postdoctoral Fellowship; Alberta Innovates Technology Futures Scholarship FX LVA held an Alberta Innovates Health Solutions Postdoctoral Fellowship. RAA is an Alberta Innovates Technology Futures Scholarship awardee. CMS is an Alberta Innovates Scholar. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 47 TC 10 Z9 10 U1 1 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 30 PY 2014 VL 9 IS 1 AR e87051 DI 10.1371/journal.pone.0087051 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 302PJ UT WOS:000330617100047 PM 24498018 ER PT J AU Dunlap, CA Schisler, DA AF Dunlap, Christopher A. Schisler, David A. TI Characterization of the Surface Properties of Wheat Spikelet Components Grown under Different Regimens and the Biocontrol Yeast Cryptococcus flavescens SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE wheat; spikelet; greenhouse; field; anthesis; Cryptococcus ID FUSARIUM HEAD BLIGHT; FIELD CONDITIONS; GIBBERELLA-ZEAE; GREENHOUSE; GRAMINEARUM; STABILITY; SELECTION; ADHESION; ENERGY; EFFICIENCY AB Surface properties play an important role in plant microbe interactions and determine if microbial propagules adhere to the surface of a plant. Fusarium head blight is an important disease of wheat that is initiated by the pathogen colonizing the wheat head. To better understand how surface properties of wheat may affect disease development and spray applications, the surface properties of wheat (Triticum aestivum L.) spikelet components were characterized under different environmental growing regimes. In addition, the surface properties of the biocontrol yeast Cryptococcus flavescens OH 182.9, which has been shown to effective in managing Fusarium head blight, were characterized. Wheat samples grown in a greenhouse environment were compared with samples produced in the field for two wheat cultivars. The results show changes occurring in the surface energy parameters and estimates of roughness during this period between the two cultivars. In general, the greenhouse samples were more hydrophobic than those grown in the field. The surface properties of the biocontrol yeast C. flavescens OH 182.9 were determined from contact angles on microbial lawns and revealed the cells were hydrophobic with a free energy of aggregation of -86.3 mJ/m(2) in water. C1 [Dunlap, Christopher A.; Schisler, David A.] ARS, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Dunlap, CA (reprint author), ARS, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. EM christopher.dunlap@ars.usda.gov FU U.S. Wheat and Barley Scab Initiative FX Studies were made possible, in part, by a grant from the U.S. Wheat and Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. The mention of firm names or trade products does not imply that they are endorsed or recommended by the USDA over other firms or similar products not mentioned. NR 36 TC 0 Z9 0 U1 1 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 29 PY 2014 VL 62 IS 4 BP 809 EP 815 DI 10.1021/jf404181f PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 302IS UT WOS:000330598700001 PM 24410183 ER PT J AU Peretto, G Du, WX Avena-Bustillos, RJ Berrios, JDJ Sambo, P McHugh, TH AF Peretto, Greta Du, Wen-Xian Avena-Bustillos, Roberto J. Berrios, Jose De J. Sambo, Paolo McHugh, Tara H. TI Optimization of Antimicrobial and Physical Properties of Alginate Coatings Containing Carvacrol and Methyl Cinnamate for Strawberry Application SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE alginate coatings; carvacrol; methyl cinnamate; antimicrobial activity; strawberry; physical properties; response surface methodology ID ESCHERICHIA-COLI O157-H7; ESSENTIAL OILS; ANTIBACTERIAL ACTIVITY; STORAGE STABILITY; EDIBLE COATINGS; FILMS; OPPORTUNITIES; RELEASE; AGENTS; FRUIT AB Increasing strawberry consumption has led to a growing safety concern because they are not washed after harvest. An antimicrobial edible coating could be an effective postharvest technique to ensure microbial safety and, at the same time, retain overall quality of the fruits. Response surface methodology was used to optimize the antimicrobial activity against Escherichia coli O157:H7 and Botrytis cinerea and several physical properties (turbidity, viscosity, and whitish index) of an alginate coating. A full factorial design was used to select the concentrations of carvacrol and methyl cinnamate on the basis of their effect against E. coli and B. cinerea. A central composite design was then performed to evaluate the effects/interactions of the two antimicrobials on the coating characteristics. The results from analysis of variance showed the significant fitting of all responses to the quadratic model. To attain the desirable responses, the optimal concentrations were 0.98% (w/w) carvacrol and 1.45% (w/w) methyl cinnamate. C1 [Peretto, Greta; Sambo, Paolo] Univ Padua, Dept Agron Food Nat Resources Anim & Environm, I-35020 Padua, Italy. [Du, Wen-Xian; Avena-Bustillos, Roberto J.; Berrios, Jose De J.; McHugh, Tara H.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Peretto, G (reprint author), Univ Padua, Dept Agron Food Nat Resources Anim & Environm, Viale Univ 16, I-35020 Padua, Italy. EM greta.peretto@gmail.com RI sambo, Paolo/B-6742-2014 OI sambo, Paolo/0000-0003-4348-8838 FU Department of Agronomy, Food, Natural Resources, Animals and Environment (DAF-NAE) of the University of Padova (Italy) FX This work was supported by the Department of Agronomy, Food, Natural Resources, Animals and Environment (DAF-NAE) of the University of Padova (Italy). NR 47 TC 8 Z9 8 U1 3 U2 37 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 29 PY 2014 VL 62 IS 4 BP 984 EP 990 DI 10.1021/jf4042886 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 302IS UT WOS:000330598700021 PM 24405047 ER PT J AU Baxter, IR Ziegler, G Lahner, B Mickelbart, MV Foley, R Danku, J Armstrong, P Salt, DE Hoekenga, OA AF Baxter, Ivan R. Ziegler, Gregory Lahner, Brett Mickelbart, Michael V. Foley, Rachel Danku, John Armstrong, Paul Salt, David E. Hoekenga, Owen A. TI Single-Kernel Ionomic Profiles Are Highly Heritable Indicators of Genetic and Environmental Influences on Elemental Accumulation in Maize Grain (Zea mays) SO PLOS ONE LA English DT Article ID PHOSPHORUS ACQUISITION; PLANTS; ASSOCIATION; ADAPTATIONS; LOCI AB The ionome, or elemental profile, of a maize kernel can be viewed in at least two distinct ways. First, the collection of elements within the kernel are food and feed for people and animals. Second, the ionome of the kernel represents a developmental end point that can summarize the life history of a plant, combining genetic programs and environmental interactions. We assert that single-kernel-based phenotyping of the ionome is an effective method of analysis, as it represents a reasonable compromise between precision, efficiency, and power. Here, we evaluate potential pitfalls of this sampling strategy using several field-grown maize sample sets. We demonstrate that there is enough genetically determined diversity in accumulation of many of the elements assayed to overcome potential artifacts. Further, we demonstrate that environmental signals are detectable through their influence on the kernel ionome. We conclude that using single kernels as the sampling unit is a valid approach for understanding genetic and environmental effects on the maize kernel ionome. C1 [Baxter, Ivan R.; Ziegler, Gregory] ARS, USDA, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO 63166 USA. [Lahner, Brett; Mickelbart, Michael V.; Foley, Rachel] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA. [Danku, John; Salt, David E.] Univ Aberdeen, Inst Biol & Environm Sci, Aberdeen, Scotland. [Armstrong, Paul] ARS, USDA, Engn & Wind Eros Res Unit, Manhattan, KS USA. [Hoekenga, Owen A.] ARS, USDA, RW Holley Ctr Agr & Hlth, Ithaca, NY USA. RP Baxter, IR (reprint author), ARS, USDA, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO 63166 USA. EM Ivan.Baxter@ars.usda.gov; Owen.Hoekenga@ars.usda.gov RI Baxter, Ivan/A-1052-2009; Danku, John/C-3477-2014 OI Baxter, Ivan/0000-0001-6680-1722; Danku, John/0000-0002-5103-3852 FU U.S. National Science Foundation, Plant Genome Research Program [IOS 0701119, IOS 1126950] FX This research was supported in part by the U.S. National Science Foundation, Plant Genome Research Program (grant # IOS 0701119 awarded to David Salt and grant # IOS 1126950 awarded to Ivan Baxter and Owen Hoekenga) and United States Department of Agriculture-Agricultural Research Service Intramural funds to Ivan Baxter. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 28 TC 10 Z9 11 U1 1 U2 24 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 29 PY 2014 VL 9 IS 1 AR e87628 DI 10.1371/journal.pone.0087628 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 301ZC UT WOS:000330570000164 PM 24489944 ER PT J AU Root, JJ Shriner, SA Bentler, KT Gidlewski, T Mooers, NL Ellis, JW Spraker, TR VanDalen, KK Sullivan, HJ Franklin, AB AF Root, J. Jeffrey Shriner, Susan A. Bentler, Kevin T. Gidlewski, Thomas Mooers, Nicole L. Ellis, Jeremy W. Spraker, Terry R. VanDalen, Kaci K. Sullivan, Heather J. Franklin, Alan B. TI Extended Viral Shedding of a Low Pathogenic Avian Influenza Virus by Striped Skunks (Mephitis mephitis) SO PLOS ONE LA English DT Article ID UNITED-STATES; INFECTION; SUBTYPES; MINK AB Background: Striped skunks (Mephitis mephitis) are susceptible to infection with some influenza A viruses. However, the viral shedding capability of this peri-domestic mammal and its potential role in influenza A virus ecology are largely undetermined. Methodology/Principal Findings: Striped skunks were experimentally infected with a low pathogenic (LP) H4N6 avian influenza virus (AIV) and monitored for 20 days post infection (DPI). All of the skunks exposed to H4N6 AIV shed large quantities of viral RNA, as detected by real-time RT-PCR and confirmed for live virus with virus isolation, from nasal washes and oral swabs (maximum <= 10(6.02) PCR EID50 equivalent/mL and <= 10(5.19) PCR EID50 equivalent/mL, respectively). Some evidence of potential fecal shedding was also noted. Following necropsy on 20 DPI, viral RNA was detected in the nasal turbinates of one individual. All treatment animals yielded evidence of a serological response by 20 DPI. Conclusions/Significance: These results indicate that striped skunks have the potential to shed large quantities of viral RNA through the oral and nasal routes following exposure to a LP AIV. Considering the peri-domestic nature of these animals, along with the duration of shedding observed in this species, their presence on poultry and waterfowl operations could influence influenza A virus epidemiology. For example, this species could introduce a virus to a naive poultry flock or act as a trafficking mechanism of AIV to and from an infected poultry flock to naive flocks or wild bird populations. C1 [Root, J. Jeffrey; Shriner, Susan A.; Bentler, Kevin T.; Mooers, Nicole L.; Ellis, Jeremy W.; VanDalen, Kaci K.; Sullivan, Heather J.; Franklin, Alan B.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Gidlewski, Thomas] Wildlife Serv, USDA, Natl Wildlife Dis Program, Ft Collins, CO USA. [Spraker, Terry R.] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. RP Root, JJ (reprint author), Wildlife Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. EM Jeff.root@aphis.usda.gov OI Shriner, Susan/0000-0003-0349-7182; Ellis, Jeremy/0000-0003-4512-0906 FU United States Department of Agriculture FX This study was funded by the United States Department of Agriculture. The manuscript was reviewed for general policy statements committing the USDA to action, but otherwise the findings were independently developed by the authors. NR 32 TC 4 Z9 4 U1 1 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 29 PY 2014 VL 9 IS 1 AR e70639 DI 10.1371/journal.pone.0070639 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 301ZC UT WOS:000330570000001 PM 24489638 ER PT J AU Chakraborty, M Kuriata, A Henderson, JN Salvucci, ME Wachter, R Levitus, M AF Chakraborty, Manas Kuriata, Agnieszka Henderson, J. Nathan Salvucci, Michael E. Wachter, Rebekka Levitus, Marcia TI ATP-Mg2+ Mediated Assembly of Rubisco Activase Investigated using Fluorescence Correlation Spectroscopy SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract CT 58th Annual Meeting of the Biophysical-Society CY FEB 15-19, 2014 CL San Francisco, CA SP Biophys Soc C1 [Chakraborty, Manas; Kuriata, Agnieszka; Henderson, J. Nathan; Wachter, Rebekka; Levitus, Marcia] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ USA. [Salvucci, Michael E.] ARS, Arid Land Agr Res Ctr, USDA, Tempe, AZ USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0006-3495 EI 1542-0086 J9 BIOPHYS J JI Biophys. J. PD JAN 28 PY 2014 VL 106 IS 2 SU 1 BP 40A EP 40A PG 1 WC Biophysics SC Biophysics GA AI6QE UT WOS:000337000400204 ER PT J AU Shoemaker, JK Keenan, TF Hollinger, DY Richardson, AD AF Shoemaker, Julie K. Keenan, Trevor F. Hollinger, David Y. Richardson, Andrew D. TI Forest ecosystem changes from annual methane source to sink depending on late summer water balance SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article DE methane; forests; carbon; climate; models ID CARBON-DIOXIDE; TEMPERATE FOREST; EDDY COVARIANCE; PINE PLANTATION; BOREAL FOREST; SOILS; CONSUMPTION; ATMOSPHERE; EMISSION; EXCHANGE AB Forests dominate the global carbon cycle, but their role in methane (CH4) biogeochemistry remains uncertain. We analyzed whole-ecosystem CH4 fluxes from 2years, obtained over a lowland evergreen forest in Maine, USA. Gross primary productivity provided the strongest correlation with the CH4 flux in both years, with an additional significant effect of soil moisture in the second, drier year. This forest was a neutral to net source of CH4 in 2011 and a small net sink in 2012. Interannual variability in the summer hydrologic cycle apparently shifts the ecosystem from being a net source to a sink for CH4. The small magnitude of the CH4 fluxes and observed control or CH4 fluxes by forest productivity and summer precipitation provide novel insight into the CH4 cycle in this globally important forest ecosystem. C1 [Shoemaker, Julie K.; Richardson, Andrew D.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Keenan, Trevor F.] Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia. [Hollinger, David Y.] USDA Forest Serv, No Res Stn, Durham, NH USA. RP Shoemaker, JK (reprint author), Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. EM jshoemak@fas.harvard.edu RI Richardson, Andrew/F-5691-2011; Hollinger, David/G-7185-2012; Keenan, Trevor/B-2744-2010 OI Richardson, Andrew/0000-0002-0148-6714; Keenan, Trevor/0000-0002-3347-0258 FU Office of Science (BER), U.S. Department of Energy FX This research was supported by the Office of Science (BER), U.S. Department of Energy. We also recognize contributions from Robert Evans, Holly Hughes, Kathleen Savage, John Lee and Eric Davidson. We thank Ankur Desai and an anonymous reviewer for their helpful comments. NR 32 TC 8 Z9 8 U1 1 U2 36 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 EI 1944-8007 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD JAN 28 PY 2014 VL 41 IS 2 BP 673 EP 679 DI 10.1002/2013GL058691 PG 7 WC Geosciences, Multidisciplinary SC Geology GA AD1JT UT WOS:000332991000068 ER PT J AU Weingartl, HM Zhang, SZ Marszal, P McGreevy, A Burton, L Wilson, WC AF Weingartl, Hana M. Zhang, Shunzhen Marszal, Peter McGreevy, Alan Burton, Lynn Wilson, William C. TI Rift Valley Fever Virus Incorporates the 78 kDa Glycoprotein into Virions Matured in Mosquito C6/36 Cells SO PLOS ONE LA English DT Article ID PROTEIN GLYCOSYLATION; EXPRESSION STRATEGY; DENDRITIC CELLS; M SEGMENT; DC-SIGN; PHLEBOVIRUS; NSM; ALPHAVIRUSES; REPLICATION; INFECTION AB Rift Valley fever virus (RVFV), genus Phlebovirus, family Bunyaviridae is a zoonotic arthropod-borne virus able to transition between distant host species, causing potentially severe disease in humans and ruminants. Viral proteins are encoded by three genomic segments, with the medium M segment coding for four proteins: nonstructural NSm protein, two glycoproteins Gn and Gc and large 78 kDa glycoprotein (LGp) of unknown function. Goat anti-RVFV polyclonal antibody and mouse monoclonal antibody, generated against a polypeptide unique to the LGp within the RVFV proteome, detected this protein in gradient purified RVFV ZH501 virions harvested from mosquito C6/36 cells but not in virions harvested from the mammalian Vero E6 cells. The incorporation of LGp into the mosquito cell line - matured virions was confirmed by immune-electron microscopy. The LGp was incorporated into the virions immediately during the first passage in C6/36 cells of Vero E6 derived virus. Our data indicate that LGp is a structural protein in C6/36 mosquito cell generated virions. The protein may aid the transmission from the mosquitoes to the ruminant host, with a possible role in replication of RVFV in the mosquito host. To our knowledge, this is a first report of different protein composition between virions formed in insect C6/36 versus mammalian Vero E6 cells. C1 [Weingartl, Hana M.; Zhang, Shunzhen; Marszal, Peter; Burton, Lynn] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB, Canada. [Weingartl, Hana M.; McGreevy, Alan] Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada. [Wilson, William C.] USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS USA. RP Weingartl, HM (reprint author), Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB, Canada. EM hana.weingartl@inspection.gc.ca FU CFIA; USDA; ARS CRIS [5430-32000-005-00D]; Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQDC-07-00982]; [CRTI-06-0138RD] FX The funding for the project was provided from the CRTI-06-0138RD project, by CFIA, the USDA, ARS CRIS project #5430-32000-005-00D, and through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Number HSHQDC-07-00982. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 29 TC 16 Z9 17 U1 0 U2 0 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 28 PY 2014 VL 9 IS 1 AR e87385 DI 10.1371/journal.pone.0087385 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 301CJ UT WOS:000330510000132 PM 24489907 ER PT J AU Wolfe, JD Johnson, MD Ralph, CJ AF Wolfe, Jared D. Johnson, Matthew D. Ralph, C. John TI Do Birds Select Habitat or Food Resources? Nearctic-Neotropic Migrants in Northeastern Costa Rica SO PLOS ONE LA English DT Article ID MIGRATING BIRDS; FOREST; AVAILABILITY; EVOLUTION; LANDBIRDS; PATTERNS; WARBLERS; PLANTS; COLOR AB Nearctic-neotropic migrant birds need to replenish energy reserves during stopover periods to successfully complete their semiannual movements. In this study we used linear models to examine the habitat use of 11 migrant species in northeastern Costa Rica to better understand the influence of food and structural resources on the presence of birds during stopover periods. Our models indicated that frugivorous migrants primarily used food abundance, while insectivorous migrants chiefly used vegetation structure as cues for habitat use during stopover. In addition to habitat use models, we documented fruiting plant phenology and found a general relationship between migrant arrival and the timing of ripe fruit availability. Our results suggest that insectivorous migrants probably rely on structural features when using habitat because it may be inherently difficult to assess cryptic-arthropod availability during a short period of time in a novel habitat, such as stopover periods. C1 [Wolfe, Jared D.] Louisiana State Univ Agr Ctr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Wolfe, Jared D.] Louisiana State Univ, Baton Rouge, LA 70803 USA. [Wolfe, Jared D.; Johnson, Matthew D.] Humboldt State Univ, Dept Wildlife, Arcata, CA 95521 USA. [Ralph, C. John] US Forest Serv, Redwood Sci Lab, USDA, Arcata, CA USA. [Ralph, C. John] Klamath Bird Observ, Ashland, OR USA. RP Wolfe, JD (reprint author), Louisiana State Univ Agr Ctr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. EM jwolfe5@lsu.edu FU United States Department of Agriculture Forest Service's Redwood Sciences Laboratory; Klamath Bird Observatory; United States Fish and Wildlife Service Richard J. Guadagno Memorial Scholarship; Stanley Harris Scholarship FX This project was funded in part by United States Department of Agriculture Forest Service's Redwood Sciences Laboratory, Klamath Bird Observatory, United States Fish and Wildlife Service Richard J. Guadagno Memorial Scholarship and the Stanley Harris Scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 41 TC 5 Z9 5 U1 2 U2 37 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 28 PY 2014 VL 9 IS 1 AR e86221 DI 10.1371/journal.pone.0086221 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 301CJ UT WOS:000330510000036 PM 24489701 ER PT J AU Fageria, NK Baligar, VC Li, YC AF Fageria, N. K. Baligar, V. C. Li, Y. C. TI NUTRIENT UPTAKE AND USE EFFICIENCY BY TROPICAL LEGUME COVER CROPS AT VARYING PH OF AN OXISOL SO JOURNAL OF PLANT NUTRITION LA English DT Article DE nutrient concentration; nutrient uptake; nutrient use efficiency; shoot dry weight ID NITROGEN MANAGEMENT; SOIL ACIDITY; GROWTH; PLANTS; CAPTURE; YIELD AB Oxisols comprise large soil group in tropical America. These soils are acidic and have low fertility. Use of tropical legume cover crops in cropping systems is an important strategy to improve fertility of these soils for sustainable crop production. Data are limited on nutrient uptake and use efficiency of tropical cover crops under different acidity levels. The objective of our study was to evaluate growth and nutrient uptake parameters of sixteen tropical legume cover crops under three soil pH (5.1, 6.5, and 7.0) of an Oxisol. Shoot dry weight was influenced significantly by pH and cover crop treatments and their interactions, indicating that cover crops used had differential responses to changing soil pH levels. Overall, shoot dry weight decreased when soil pH was raised from 5.1 to 7.0, indicating acidity tolerance of cover crops. Nutrient concentration (content per unit of dry weight), uptake (concentration X dry weight), and nutrient use efficiency (dry weight of shoot per unit of nutrient uptake) varied significantly among cover crops. The variation in nutrient uptake and use efficiency among cover crop species was associated with variation in shoot dry matter production. Significant variation among crop species in dry matter production and low C/N ratios (average value of 14.25) suggest that cover crops which produced higher dry matter yield like white jack bean, gray mucuna bean, black mucuna bean, mucuna bean ana, and lablab are important choices for planting in tropical soils to recover large amount of macro and micronutrients, and to prevent such nutrient leaching in soil plant systems. C1 [Fageria, N. K.] EMBRAPA, Natl Rice & Bean Res Ctr, BR-75375000 San Antonio De Goias, Go, Brazil. [Baligar, V. C.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Li, Y. C.] Univ Florida, Ctr Trop Res & Educ, Dept Soil & Water Sci, Homestead, FL 33031 USA. RP Fageria, NK (reprint author), EMBRAPA, Natl Rice & Bean Res Ctr, Caixa Postal 179, BR-75375000 San Antonio De Goias, Go, Brazil. EM nand.fageria@embrapa.br NR 48 TC 0 Z9 0 U1 2 U2 17 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 0190-4167 EI 1532-4087 J9 J PLANT NUTR JI J. Plant Nutr. PD JAN 28 PY 2014 VL 37 IS 2 BP 294 EP 311 DI 10.1080/01904167.2013.851695 PG 18 WC Plant Sciences SC Plant Sciences GA 290TA UT WOS:000329780000013 ER PT J AU Goettel, W Liu, ZR Xia, J Zhang, WX Zhao, PX An, YQ AF Goettel, Wolfgang Liu, Zongrang Xia, Jing Zhang, Weixiong Zhao, Patrick X. An, Yong-Qiang (Charles) TI Systems and Evolutionary Characterization of MicroRNAs and Their Underlying Regulatory Networks in Soybean Cotyledons SO PLOS ONE LA English DT Article ID SMALL RNAS; ARABIDOPSIS-THALIANA; TARGET GENES; MIRNA GENES; GLYCINE-MAX; PLANT; IDENTIFICATION; GENOME; SIRNAS; MIR159 AB MicroRNAs (miRNAs) are an emerging class of small RNAs regulating a wide range of biological processes. Soybean cotyledons evolved as sink tissues to synthesize and store seed reserves which directly affect soybean seed yield and quality. However, little is known about miRNAs and their regulatory networks in soybean cotyledons. We sequenced 292 million small RNA reads expressed in soybean cotyledons, and discovered 130 novel miRNA genes and 72 novel miRNA families. The cotyledon miRNAs arose at various stages of land plant evolution. Evolutionary analysis of the miRNA genes in duplicated genome segments from the recent Glycine whole genome duplication revealed that the majority of novel soybean cotyledon miRNAs were young, and likely arose after the duplication event 13 million years ago. We revealed the evolutionary pathway of a soybean cotyledon miRNA family (soy-miR15/ 49) that evolved from a neutral invertase gene through an inverted duplication and a series of DNA amplification and deletion events. A total of 304 miRNA genes were expressed in soybean cotyledons. The miRNAs were predicted to target 1910 genes, and form complex miRNA networks regulating a wide range of biological pathways in cotyledons. The comprehensive characterization of the miRNAs and their underlying regulatory networks at gene, pathway and system levels provides a foundation for further studies of miRNAs in cotyledons. C1 [Goettel, Wolfgang; An, Yong-Qiang (Charles)] ARS, USDA, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO 63120 USA. [Liu, Zongrang] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. [Xia, Jing; Zhang, Weixiong] Washington Univ, Dept Comp Sci & Engn, St Louis, MO USA. [Zhao, Patrick X.] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73402 USA. RP An, YQ (reprint author), ARS, USDA, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO 63120 USA. EM yong-qiang.an@ars.usda.gov FU USDA-ARS; United Soybean Board FX This research is supported by funds from USDA-ARS and the United Soybean Board to Yong-qiang Charles An. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 66 TC 12 Z9 52 U1 0 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 27 PY 2014 VL 9 IS 1 AR e86153 DI 10.1371/journal.pone.0086153 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 301BI UT WOS:000330507300040 PM 24475082 ER PT J AU Marcolino-Gomes, J Rodrigues, FA Fuganti-Pagliarini, R Bendix, C Nakayama, TJ Celaya, B Molinari, HBC de Oliveira, MCN Harmon, FG Nepomuceno, A AF Marcolino-Gomes, Juliana Rodrigues, Fabiana Aparecida Fuganti-Pagliarini, Renata Bendix, Claire Nakayama, Thiago Jonas Celaya, Brandon Correa Molinari, Hugo Bruno Neves de Oliveira, Maria Cristina Harmon, Frank G. Nepomuceno, Alexandre TI Diurnal Oscillations of Soybean Circadian Clock and Drought Responsive Genes SO PLOS ONE LA English DT Article ID REAL-TIME PCR; ABSCISIC-ACID; TRANSCRIPTION FACTOR; GLYCINE-MAX; SIGNAL-TRANSDUCTION; TRANSGENIC TOBACCO; STRESS-RESPONSE; WATER-DEFICIT; ARABIDOPSIS; PLANTS AB Rhythms produced by the endogenous circadian clock play a critical role in allowing plants to respond and adapt to the environment. While there is a well-established regulatory link between the circadian clock and responses to abiotic stress in model plants, little is known of the circadian system in crop species like soybean. This study examines how drought impacts diurnal oscillation of both drought responsive and circadian clock genes in soybean. Drought stress induced marked changes in gene expression of several circadian clock-like components, such as LCL1-, GmELF4- and PRR-like genes, which had reduced expression in stressed plants. The same conditions produced a phase advance of expression for the GmTOC1-like, GmLUX-like and GmPRR7-like genes. Similarly, the rhythmic expression pattern of the soybean drought-responsive genes DREB-, bZIP-, GOLS-, RAB18- and Remorin-like changed significantly after plant exposure to drought. In silico analysis of promoter regions of these genes revealed the presence of cis-elements associated both with stress and circadian clock regulation. Furthermore, some soybean genes with upstream ABRE elements were responsive to abscisic acid treatment. Our results indicate that some connection between the drought response and the circadian clock may exist in soybean since (i) drought stress affects gene expression of circadian clock components and (ii) several stress responsive genes display diurnal oscillation in soybeans. C1 [Marcolino-Gomes, Juliana; Rodrigues, Fabiana Aparecida; Fuganti-Pagliarini, Renata; Neves de Oliveira, Maria Cristina; Nepomuceno, Alexandre] Brazilian Agr Res Corp, Embrapa Soybean, Londrina, Parana, Brazil. [Marcolino-Gomes, Juliana] Univ Estadual Londrina, Dept Biol, Londrina, Parana, Brazil. [Bendix, Claire; Celaya, Brandon; Harmon, Frank G.] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [Bendix, Claire; Celaya, Brandon; Harmon, Frank G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Nakayama, Thiago Jonas] Univ Fed Vicosa, Dept Crop Sci, Vicosa, MG, Brazil. [Correa Molinari, Hugo Bruno; Nepomuceno, Alexandre] USDA ARS, Ctr Plant Gene Express, Embrapa LABEX US Plant Biotechnol, Albany, CA 94710 USA. RP Nepomuceno, A (reprint author), Brazilian Agr Res Corp, Embrapa Soybean, Londrina, Parana, Brazil. EM alexandre.nepomuceno@embrapa.br OI Bendix, Claire/0000-0002-4723-8652; Fuganti-Pagliarini, Renata /0000-0001-9282-2826 FU Embrapa LABEX USA program (Embrapa) [10200.10/0215-9]; CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) [8075/11-5]; an agency of the Ministry of Education of Brazil. Renata Fuganti-Pagliarini [202639/2011-8]; CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [202211/2011-8] FX This work was supported by Embrapa LABEX USA program (Embrapa cod. 10200.10/0215-9). Juliana Marcolino-Gomes was supported by a scholarship from the CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) (Process #8075/11-5), an agency of the Ministry of Education of Brazil. Renata Fuganti-Pagliarini (Process #202639/2011-8) and Fabiana A. Rodrigues (Process #202211/2011-8) were supported by scholarships from CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 80 TC 18 Z9 19 U1 2 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 27 PY 2014 VL 9 IS 1 AR e86402 DI 10.1371/journal.pone.0086402 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 301BI UT WOS:000330507300061 PM 24475115 ER PT J AU Soliai, MM Meyer, SE Udall, JA Elzinga, DE Hermansen, RA Bodily, PM Hart, AA Coleman, CE AF Soliai, Marcus M. Meyer, Susan E. Udall, Joshua A. Elzinga, David E. Hermansen, Russell A. Bodily, Paul M. Hart, Aaron A. Coleman, Craig E. TI De novo Genome Assembly of the Fungal Plant Pathogen Pyrenophora semeniperda SO PLOS ONE LA English DT Article ID REPENTIS-WHEAT INTERACTION; DIELS-ALDER REACTION; TRITICI-REPENTIS; TRANSPOSABLE ELEMENTS; BROMUS-TECTORUM; TAN SPOT; CHROMOSOME REARRANGEMENTS; GENE-EXPRESSION; TOXB GENE; SEQUENCE AB Pyrenophora semeniperda (anamorph Drechslera campulata) is a necrotrophic fungal seed pathogen that has a wide host range within the Poaceae. One of its hosts is cheatgrass (Bromus tectorum), a species exotic to the United States that has invaded natural ecosystems of the Intermountain West. As a natural pathogen of cheatgrass, P. semeniperda has potential as a biocontrol agent due to its effectiveness at killing seeds within the seed bank; however, few genetic resources exist for the fungus. Here, the genome of P. semeniperda isolate assembled from sequence reads of 454 pyrosequencing is presented. The total assembly is 32.5 Mb and includes 11,453 gene models encoding putative proteins larger than 24 amino acids. The models represent a variety of putative genes that are involved in pathogenic pathways typically found in necrotrophic fungi. In addition, extensive rearrangements, including inter-and intrachromosomal rearrangements, were found when the P. semeniperda genome was compared to P. tritici-repentis, a related fungal species. C1 [Soliai, Marcus M.; Udall, Joshua A.; Elzinga, David E.; Hermansen, Russell A.; Bodily, Paul M.; Hart, Aaron A.; Coleman, Craig E.] Brigham Young Univ, Provo, UT 84602 USA. [Meyer, Susan E.] US Forest Serv, USDA, Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT USA. RP Coleman, CE (reprint author), Brigham Young Univ, Provo, UT 84602 USA. EM craig_coleman@byu.edu FU CSREES, National Research Initiative, United States Department of Agriculture [2008-35320-18677] FX This research was funded by grant 2008-35320-18677 from CSREES, National Research Initiative, United States Department of Agriculture. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 73 TC 7 Z9 7 U1 1 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 27 PY 2014 VL 9 IS 1 AR e87045 DI 10.1371/journal.pone.0087045 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 301BI UT WOS:000330507300129 PM 24475219 ER PT J AU Noyszewski, AK Ghavami, F Alnemer, LM Soltani, A Gu, YQ Huo, NX Meinhardt, S Kianian, PMA Kianian, SF AF Noyszewski, Andrzej K. Ghavami, Farhad Alnemer, Loai M. Soltani, Ali Gu, Yong Q. Huo, Naxin Meinhardt, Steven Kianian, Penny M. A. Kianian, Shahryar F. TI Accelerated evolution of the mitochondrial genome in an alloplasmic line of durum wheat SO BMC GENOMICS LA English DT Article DE Aegilops longissima; Triticum turgidum; Heteroplasmy; Paternal leakage; atp6; Next generation sequencing; Cytoplasmic male sterility; Alloplasmic line ID CYTOPLASMIC MALE-STERILITY; ARABIDOPSIS-THALIANA; COMPLETE SEQUENCE; PATERNAL LEAKAGE; PLASMON ANALYSIS; TRITICUM WHEAT; READING FRAME; COMMON WHEATS; DNA; HETEROPLASMY AB Background: Wheat is an excellent plant species for nuclear mitochondrial interaction studies due to availability of large collection of alloplasmic lines. These lines exhibit different vegetative and physiological properties than their parents. To investigate the level of sequence changes introduced into the mitochondrial genome under the alloplasmic condition, three mitochondrial genomes of the Triticum-Aegilops species were sequenced: 1) durum alloplasmic line with the Ae. longissima cytoplasm that carries the T. turgidum nucleus designated as (lo) durum, 2) the cytoplasmic donor line, and 3) the nuclear donor line. Results: The mitochondrial genome of the T. turgidum was 451,678 bp in length with high structural and nucleotide identity to the previously characterized T. aestivum genome. The assembled mitochondrial genome of the (lo) durum and the Ae. longissima were 431,959 bp and 399,005 bp in size, respectively. The high sequence coverage for all three genomes allowed analysis of heteroplasmy within each genome. The mitochondrial genome structure in the alloplasmic line was genetically distant from both maternal and paternal genomes. The alloplasmic durum and the Ae. longissima carry the same versions of atp6, nad6, rps19-p, cob and cox2 exon 2 which are different from the T. turgidum parent. Evidence of paternal leakage was also observed by analyzing nad9 and orf359 among all three lines. Nucleotide search identified a number of open reading frames, of which 27 were specific to the (lo) durum line. Conclusions: Several heteroplasmic regions were observed within genes and intergenic regions of the mitochondrial genomes of all three lines. The number of rearrangements and nucleotide changes in the mitochondrial genome of the alloplasmic line that have occurred in less than half a century was significant considering the high sequence conservation between the T. turgidum and the T. aestivum that diverged from each other 10,000 years ago. We showed that the changes in genes were not limited to paternal leakage but were sufficiently significant to suggest that other mechanisms, such as recombination and mutation, were responsible. The newly formed ORFs, differences in gene sequences and copy numbers, heteroplasmy, and substoichiometric changes show the potential of the alloplasmic condition to accelerate evolution towards forming new mitochondrial genomes. C1 [Noyszewski, Andrzej K.; Soltani, Ali; Kianian, Penny M. A.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Ghavami, Farhad] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Alnemer, Loai M.] Univ Jordan, Comp Informat Syst Dept, Amman, Jordan. [Gu, Yong Q.; Huo, Naxin] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Meinhardt, Steven] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Kianian, Shahryar F.] ARS, Cereal Dis Lab, USDA, Minneapolis, MN 55108 USA. RP Kianian, SF (reprint author), ARS, Cereal Dis Lab, USDA, Minneapolis, MN 55108 USA. EM Shahryar.Kianian@ars.usda.gov FU United States Army Research Office [W911NF-08-1-0319] FX The authors wish to thank Drs. Justin Faris and David Horvath for careful review of the manuscript and valuable comments. The authors also wish to thank Dr. Gerard Lazo, Justin Hegstad and Allen Peckrul for their technical support throughout the research. This work was supported by funding from the United States Army Research Office grant No. W911NF-08-1-0319 to S.F. Kianian as the PI. NR 58 TC 4 Z9 4 U1 2 U2 15 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JAN 25 PY 2014 VL 15 AR 67 DI 10.1186/1471-2164-15-67 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AC5PM UT WOS:000332572900001 PM 24460856 ER PT J AU Cleveland, BM Weber, GM AF Cleveland, Beth M. Weber, Gregory M. TI Ploidy effects on genes regulating growth mechanisms during fasting and refeeding in juvenile rainbow trout (Oncorhynchus mykiss) SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Article DE Compensatory growth; GeXP; Gene dosage; Gene compensation; Fish ID IGF-BINDING PROTEINS; SKELETAL-MUSCLE; FACTOR-I; PERIPHERAL REGULATION; COMPENSATORY GROWTH; LIVER-REGENERATION; SEXUAL-MATURATION; COHO SALMON; INSULIN; HORMONE AB Diploid and triploid rainbow trout weighing approximately 3 g were either fed for five weeks, or feed deprived for one week, followed by refeeding. During feed deprivation gastrointestinal somatic index decreased in diploids, but not triploids, and during refeeding, carcass growth rate recovered more quickly in triploids. Although not affected by ploidy, liver ghr2 and igfbp2b expression increased and igfbpl b decreased in fasted fish. Effects of ploidy on gene expression indicate potential mechanisms associated with improved recovery growth in triploids, which include decreased hepatic igfbp expression, which could influence IGF-I bioavailability, differences in tissue sensitivity to TGFbeta ligands due to altered tgibr and smad expression, and differences in expression of muscle regulatory genes (myf5, mstnla, and mstnlb). These data suggest that polyploidy influences the expression of genes critical to muscle development and general growth regulation, which may explain why triploid fish recover from nutritional insult better than diploid fish. Published by Elsevier Ireland Ltd. C1 [Cleveland, Beth M.; Weber, Gregory M.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25427 USA. RP Cleveland, BM (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25427 USA. EM beth.cleveland@ars.usda.gov FU Agricultural Research Service Project [1930-31000-010-000D] FX The authors are grateful to Lisa Radler and Jill Birkett for their technical expertise during sample collection and analysis and to David Payne for his expertise during the execution of the study. In addition, we acknowledge the technical and animal caretaking contributions from Josh Kretzer, Jenea McGowan, and Kyle Jenkins. Mention of trade names is solely for the purpose of providing accurate information and should not imply product endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. Funding for this study came from the Agricultural Research Service Project 1930-31000-010-000D. NR 57 TC 7 Z9 7 U1 4 U2 31 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD JAN 25 PY 2014 VL 382 IS 1 BP 139 EP 149 DI 10.1016/j.mce.2013.09.024 PG 11 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA 299UQ UT WOS:000330421600015 PM 24076188 ER PT J AU Coates, BS Fraser, LM French, BW Sappington, TW AF Coates, Brad S. Fraser, Lisa M. French, B. Wade Sappington, Thomas W. TI Proliferation and copy number variation of BEL-like long terminal repeat retrotransposons within the Diabrotica virgifera virgifera genome SO GENE LA English DT Article DE Genome variation; Integration/excision mutation; Corn rootworm ID WESTERN CORN-ROOTWORM; EXPRESSED SEQUENCE TAGS; PAO-LIKE ELEMENTS; TRANSPOSABLE ELEMENTS; DROSOPHILA-MELANOGASTER; SACCHAROMYCES-CEREVISIAE; CAENORHABDITIS-ELEGANS; PARATHION RESISTANCE; BOMBYX-MORI; EVOLUTION AB The proliferation of retrotransposons within a genome can contribute to increased size and affect the function of eukaryotic genes. BEL/Pao-like long-terminal repeat (LTR) retrotransposons were annotated from the highly adaptable insect species Diabrotica virgifera virgifera, the Western corn rootworm, using survey sequences from bacterial artificial chromosome (BAC) inserts and contigs derived from a low coverage next-generation genome sequence assembly. Eleven unique D. v. virgifera BEL elements were identified that contained full-length gag-pol coding sequences, whereas 88 different partial coding regions were characterized from partially assembled elements. Estimated genome copy number for full and partial BEL-like elements ranged from similar to 8 to 1582 among individual contigs using a normalized depth of coverage (DOC) among Illumina HiSeq reads (total genome copy number similar to 8821). BEL element copy number was correlated among different D. v. virgifera populations (R-2 = 0.9846), but individual element numbers varied <= 1.68-fold and the total number varied by similar to 527 copies. These data indicate that BEL element proliferation likely contributed to a large genome size, and suggest that differences in copy number are a source of genetic variability among D. v. virgifera. Published by Elsevier B.V. C1 [Coates, Brad S.; Sappington, Thomas W.] Iowa State Univ, USDA, ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Coates, Brad S.; Sappington, Thomas W.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Fraser, Lisa M.] Iowa State Univ, Dept Biomed Sci, Ames, IA 50011 USA. [French, B. Wade] ARS, USDA, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Coates, BS (reprint author), Iowa State Univ, USDA, ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. EM brad.coates@ars.usda.gov; Imfraser@iastate.edu; wade.french@ars.usda.gov; tom.sapptington@ars.usda.gov FU USDA, Agricultural Research Service (CRIS) [3625-22000-017-00D]; Iowa Agriculture and Home Economics Experiment Station, Iowa State University, Ames, IA [3543] FX This research was a joint contribution from the USDA, Agricultural Research Service (CRIS Project 3625-22000-017-00D), and the Iowa Agriculture and Home Economics Experiment Station, Iowa State University, Ames, IA (Project 3543). The authors would like to thank USDA-ARS Technicians, Robert Gunnarson and Chad Nielson, for performing associated laboratory protocols, and collecting and rearing of beetles. This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation by USDA for their use. NR 68 TC 1 Z9 2 U1 1 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD JAN 25 PY 2014 VL 534 IS 2 BP 362 EP 370 DI 10.1016/j.gene.2013.09.100 PG 9 WC Genetics & Heredity SC Genetics & Heredity GA 293ZZ UT WOS:000330013500031 PM 24498652 ER PT J AU Wong, WW Mikhail, C Ortiz, CL Lathan, D Moore, LA Konzelmann, KL Smith, EO AF Wong, William W. Mikhail, Carmen Ortiz, Christina L. Lathan, Debra Moore, Louis A. Konzelmann, Karen L. Smith, E. O'Brian TI Body weight has no impact on self-esteem of minority children living in inner city, low-income neighborhoods: a cross-sectional study SO BMC PEDIATRICS LA English DT Article DE Self-esteem; Children; Minority; Obesity ID OBESE CHILDREN; AFRICAN-AMERICAN; MASS INDEX; PERCEPTION PROFILE; US CHILDREN; ADOLESCENTS; RISK; OVERWEIGHT; SMOKING; PREVALENCE AB Background: The relationship between body weight and self-esteem among underserved minority children is not well documented. Methods: We measured the self-esteem profile using the Self-Perception Profile for Children among 910 minority children at 17 Houston community centers. Results: Weight status had no effect on any of the self-esteem scores among the minority children (P >= 0.21). Black children had higher scholastic competence than Hispanic children (P = 0.05). Social acceptance was not affected by age, gender, and race/ethnicity (P >= 0.13). Significant age x gender (P = 0.006) and race x gender (P = 0.005) interactions were detected on athletic competence. The younger boys had higher athletic competence than the younger and older girls (P <= 0.01). The older boys had higher athletic competence than the older girls (P = 0.008) but their scores were not different from those of the younger girls (P = 0.07). Within each race/ethnicity group, boys had higher athletic competence than girls (P <= 0.03). Black boys had higher athletic competence than Hispanic girls (P = 0.007) but their scores were not different from those of the Hispanic boys (P = 0.08). Age and gender had no effect on physical appearance but black children had higher scores than Hispanic children (P = 0.05). Behavioral conduct was not affected by age, gender, or race/ethnicity (P >= 0.11). There was an age x gender interaction on global self-worth (P = 0.02) with boys having similar scores regardless of ages (P = 0.40) or ethnicity (P = 0.98). However, boys from both age groups had higher global self-worth than the older girls (P <= 0.04) but their scores were not different from those of the younger girls (P = 0.07). Conclusions: For the first time, we documented that being normal weight did not necessarily guarantee positive self-esteem among minority children. Their self-esteem scores were similar to those found among children who were diagnosed with obesity and obesity-related co-morbidities and lower than those reported among normal-weight white children. Therefore, activities to promote self-esteem are important when working with underserved minority children in order to promote a healthy lifestyle. C1 [Wong, William W.; Konzelmann, Karen L.; Smith, E. O'Brian] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Wong, William W.; Konzelmann, Karen L.; Smith, E. O'Brian] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Mikhail, Carmen] Texas Childrens Hosp, Baylor Coll Med, Houston, TX 77030 USA. [Wong, William W.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Ortiz, Christina L.; Lathan, Debra; Moore, Louis A.] Houston Pk & Recreat Dept, Houston, TX USA. RP Wong, WW (reprint author), Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. EM wwong@bcm.edu FU National Research Initiative of the USDA National Institute of Food and Agriculture [2008-55215-18875]; USDA/ARS [6250-51000-053] FX The study was funded by the National Research Initiative of the USDA National Institute of Food and Agriculture grant #2008-55215-18875 with support from the USDA/ARS grant #6250-51000-053. The study sponsors had no involvement in the study design; the collection, analysis, and interpretation of data; the writing of the manuscript; and the decision to submit the paper for publication. NR 49 TC 2 Z9 2 U1 1 U2 4 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2431 J9 BMC PEDIATR JI BMC Pediatr. PD JAN 24 PY 2014 VL 14 AR 19 DI 10.1186/1471-2431-14-19 PG 8 WC Pediatrics SC Pediatrics GA AG1PW UT WOS:000335189000001 PM 24456638 ER PT J AU Spencer, SP Wilhelm, C Yang, Q Hall, JA Bouladoux, N Boyd, A Nutman, TB Urban, JF Wang, J Ramalingam, TR Bhandoola, A Wynn, TA Belkaid, Y AF Spencer, S. P. Wilhelm, C. Yang, Q. Hall, J. A. Bouladoux, N. Boyd, A. Nutman, T. B. Urban, J. F., Jr. Wang, J. Ramalingam, T. R. Bhandoola, A. Wynn, T. A. Belkaid, Y. TI Adaptation of Innate Lymphoid Cells to a Micronutrient Deficiency Promotes Type 2 Barrier Immunity SO SCIENCE LA English DT Article ID VITAMIN-A-DEFICIENCY; RETINOIC ACID; HELMINTH INFECTION; T-CELLS; RESPONSES; INFLAMMATION; INTESTINE; CHILDREN; ALPHA; IL-13 AB How the immune system adapts to malnutrition to sustain immunity at barrier surfaces, such as the intestine, remains unclear. Vitamin A deficiency is one of the most common micronutrient deficiencies and is associated with profound defects in adaptive immunity. Here, we found that type 3 innate lymphoid cells (ILC3s) are severely diminished in vitamin A-deficient settings, which results in compromised immunity to acute bacterial infection. However, vitamin A deprivation paradoxically resulted in dramatic expansion of interleukin-13 (IL-13)-producing ILC2s and resistance to nematode infection in mice, which revealed that ILCs are primary sensors of dietary stress. Further, these data indicate that, during malnutrition, a switch to innate type 2 immunity may represent a powerful adaptation of the immune system to promote host survival in the face of ongoing barrier challenges. C1 [Spencer, S. P.; Wilhelm, C.; Hall, J. A.; Bouladoux, N.; Ramalingam, T. R.; Wynn, T. A.; Belkaid, Y.] NIAID, Immun Barrier Sites Initiat, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. [Spencer, S. P.; Wilhelm, C.; Hall, J. A.; Bouladoux, N.; Belkaid, Y.] NIAID, Mucosal Immunol Sect, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. [Spencer, S. P.; Yang, Q.; Bhandoola, A.] Univ Penn, Dept Pathol & Lab Med, Inst Immunol, Perelman Sch Med, Philadelphia, PA 19104 USA. [Boyd, A.; Nutman, T. B.] NIAID, Helminth Immunol Sect, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. [Urban, J. F., Jr.] USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. [Wang, J.] Univ Calif Berkeley, Program Metab Biol Nutr Sci & Toxicol, Berkeley, CA 94720 USA. [Ramalingam, T. R.; Wynn, T. A.] NIAID, Immunopathogenesis Sect, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. RP Belkaid, Y (reprint author), NIAID, Immun Barrier Sites Initiat, Parasit Dis Lab, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM ybelkaid@niaid.nih.gov OI Urban, Joseph/0000-0002-1590-8869 FU Division of Intramural Research of the National Institute of Allergy and Infectious Diseases (NIAID), NIH; Office of Dietary Supplements, NIH; NIH [F30 DK094708]; Human Frontier Science Program; U.S. Department of Agriculture-Agricultural Research Service [1254-32000-094-00D]; Damon Runyon Cancer Research Foundation FX This work was supported by the Division of Intramural Research of the National Institute of Allergy and Infectious Diseases (NIAID), NIH; Office of Dietary Supplements, NIH; NIH grant F30 DK094708 (S.P.S.); Human Frontier Science Program (C.W.); U.S. Department of Agriculture-Agricultural Research Service project plan #1254-32000-094-00D (J.U.); and by the Damon Runyon Cancer Research Foundation (Dale F. and Betty Ann Frey Fellow, J.A.H.). We thank the NIAID animal facility staff; K. Holmes and the NIAID sorting facility, in particular, C. Eigsti and E. Stregevsky; and K. Beacht and the NIAID gnotobiotic facility, in particular, C. Avecedo and D. Trageser-Cesler for technical assistance. We thank D. Artis for providing C. rodentium, W. J. Leonard for providing TSLPR-/- mice, and W. Paul for providing IL33R-/- mice. We thank the Belkaid lab for critical discussions regarding the manuscript. The data presented in this manuscript are tabulated in the main paper and the supplementary materials. NR 33 TC 120 Z9 126 U1 4 U2 23 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD JAN 24 PY 2014 VL 343 IS 6169 BP 432 EP 437 DI 10.1126/science.1247606 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 294IR UT WOS:000330039300053 PM 24458645 ER PT J AU Jones, JS Tullis, JA Haavik, LJ Guldin, JM Stephen, FM AF Jones, Joshua S. Tullis, Jason A. Haavik, Laurel J. Guldin, James M. Stephen, Fred M. TI Monitoring oak-hickory forest change during an unprecedented red oak borer outbreak in the Ozark Mountains: 1990 to 2006 SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE change detection; Landsat TM/ETM; normalized difference water index; in situ data; insect outbreak ID ENAPHALODES-RUFULUS COLEOPTERA; NORTHERN ARKANSAS; MISSOURI OZARKS; CERAMBYCIDAE; DECLINE; MORTALITY; HALDEMAN; SCARLET; BLACK; STAND AB Upland oak-hickory forests in Arkansas, Missouri, and Oklahoma experienced oak decline in the late 1990s and early 2000s during an unprecedented outbreak of a native beetle, the red oak borer (ROB), Enaphalodes rufulus (Haldeman). Although remote sensing supports frequent monitoring of continuously changing forests, comparable in situ observations are critical for developing an understanding of past and potential ROB damage in the Ozark Mountains. We categorized forest change using a normalized difference water index (NDWI) applied to multi-temporal Landsat TM and ETM+ imagery (1990, 2001, and 2006). Levels of decline or growth were categorized using simple statistical thresholds of change in the NDWI over time. Corresponding decline and growth areas were then observed in situ where tree diameter, age, crown condition, and species composition were measured within variable radius plots. Using a machine learning decision tree classifier, remote sensing-derived decline and growth was characterized in terms of in situ observation. Plots with tree quadratic mean diameter at breast height >= 21.5 cm were categorized remotely as in severe decline. Landsat TM/ETM+-based NDWI derivatives reveal forest decline and regrowth in post-ROB outbreak surveys. Historical and future Landsat-based canopy change detection should be incorporated with existing landscape-based prediction of ROB hazard. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. C1 [Jones, Joshua S.; Haavik, Laurel J.; Stephen, Fred M.] Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. [Tullis, Jason A.] Univ Arkansas, Dept Geosci, Fayetteville, AR 72701 USA. [Tullis, Jason A.] Univ Arkansas, Ctr Adv Spatial Technol, Fayetteville, AR 72701 USA. [Haavik, Laurel J.] Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2E5, Canada. [Guldin, James M.] USDA, Forest Serv Soothern Res Stn, Hot Springs, AR 71902 USA. RP Jones, JS (reprint author), Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. EM jsjgis@gmail.com OI Haavik, Laurel/0000-0002-7749-9095 FU USDA Forest Service Southern Research Station "Applied Silvicultural Assessment of Upland Oak-Hickory Forests and the Red Oak Borer in the Ozark and Ouachita Mountains of Arkansas"; USGS award [08HQGR0157] FX This study was supported by the USDA Forest Service Southern Research Station "Applied Silvicultural Assessment of Upland Oak-Hickory Forests and the Red Oak Borer in the Ozark and Ouachita Mountains of Arkansas" and by USGS award number 08HQGR0157 "America View: A National Remote Sensing Consortium." The authors directed the design, analysis, and preparation of published material and data. NR 44 TC 4 Z9 4 U1 3 U2 26 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD JAN 23 PY 2014 VL 8 AR 083687 DI 10.1117/1.JRS.8.083687 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 301XZ UT WOS:000330566900001 ER PT J AU Brudvig, LA Orrock, JL Damschen, EI Collins, CD Hahn, PG Mattingly, WB Veldman, JW Walker, JL AF Brudvig, Lars A. Orrock, John L. Damschen, Ellen I. Collins, Cathy D. Hahn, Philip G. Mattingly, W. Brett Veldman, Joseph W. Walker, Joan L. TI Land-Use History and Contemporary Management Inform an Ecological Reference Model for Longleaf Pine Woodland Understory Plant Communities SO PLOS ONE LA English DT Article ID RESTORATION ECOLOGY; PALUSTRIS ECOSYSTEMS; REGRESSION TREES; FIRE; BIODIVERSITY; FORESTS; PLANTATIONS; VEGETATION; SAVANNA; PRODUCTIVITY AB Ecological restoration is frequently guided by reference conditions describing a successfully restored ecosystem; however, the causes and magnitude of ecosystem degradation vary, making simple knowledge of reference conditions insufficient for prioritizing and guiding restoration. Ecological reference models provide further guidance by quantifying reference conditions, as well as conditions at degraded states that deviate from reference conditions. Many reference models remain qualitative, however, limiting their utility. We quantified and evaluated a reference model for southeastern U.S. longleaf pine woodland understory plant communities. We used regression trees to classify 232 longleaf pine woodland sites at three locations along the Atlantic coastal plain based on relationships between understory plant community composition, soils (which broadly structure these communities), and factors associated with understory degradation, including fire frequency, agricultural history, and tree basal area. To understand the spatial generality of this model, we classified all sites together and for each of three study locations separately. Both the regional and location-specific models produced quantifiable degradation gradients-i.e., progressive deviation from conditions at 38 reference sites, based on understory species composition, diversity and total cover, litter depth, and other attributes. Regionally, fire suppression was the most important degrading factor, followed by agricultural history, but at individual locations, agricultural history or tree basal area was most important. At one location, the influence of a degrading factor depended on soil attributes. We suggest that our regional model can help prioritize longleaf pine woodland restoration across our study region; however, due to substantial landscape-to-landscape variation, local management decisions should take into account additional factors (e. g., soil attributes). Our study demonstrates the utility of quantifying degraded states and provides a series of hypotheses for future experimental restoration work. More broadly, our work provides a framework for developing and evaluating reference models that incorporate multiple, interactive anthropogenic drivers of ecosystem degradation. C1 [Brudvig, Lars A.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Orrock, John L.; Damschen, Ellen I.; Hahn, Philip G.; Mattingly, W. Brett; Veldman, Joseph W.] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. [Collins, Cathy D.] Colby Coll, Dept Biol, Waterville, ME 04901 USA. [Walker, Joan L.] US Forest Serv, Southern Res Stn, Clemson, SC USA. RP Brudvig, LA (reprint author), Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. EM brudvig@msu.edu FU Strategic Environmental Research and Development Program [RC-1695]; Department of Energy, Aiken, SC [DE-AI09-00SR22188] FX This study was funded by the Strategic Environmental Research and Development Program (Project RC-1695) and by funds provided to the Department of Agriculture, Forest Service, Savannah River, under Interagency Agreement DE-AI09-00SR22188 with the Department of Energy, Aiken, SC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 54 TC 10 Z9 10 U1 6 U2 70 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 23 PY 2014 VL 9 IS 1 AR e86604 DI 10.1371/journal.pone.0086604 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297XI UT WOS:000330288000120 PM 24466167 ER PT J AU Kudapa, H Azam, S Sharpe, AG Taran, B Li, R Deonovic, B Cameron, C Farmer, AD Cannon, SB Varshney, RK AF Kudapa, Himabindu Azam, Sarwar Sharpe, Andrew G. Taran, Bunyamin Li, Rong Deonovic, Benjamin Cameron, Connor Farmer, Andrew D. Cannon, Steven B. Varshney, Rajeev K. TI Comprehensive Transcriptome Assembly of Chickpea (Cicer arietinum L.) Using Sanger and Next Generation Sequencing Platforms: Development and Applications SO PLOS ONE LA English DT Article ID CAJANUS-CAJAN L.; TERMINAL DROUGHT STRESS; DRAFT GENOME SEQUENCE; GENE DISCOVERY; EST DATABASES; SSR-MARKERS; CROP; RESOURCE; LEGUMES; IMPROVEMENT AB A comprehensive transcriptome assembly of chickpea has been developed using 134.95 million Illumina single-end reads, 7.12 million single-end FLX/454 reads and 139,214 Sanger expressed sequence tags (ESTs) from >17 genotypes. This hybrid transcriptome assembly, referred to as Cicer arietinum Transcriptome Assembly version 2 (CaTA v2, available at http://data.comparative-legumes.org/transcriptomes/cicar/lista_cicar-201201), comprising 46,369 transcript assembly contigs (TACs) has an N50 length of 1,726 bp and a maximum contig size of 15,644 bp. Putative functions were determined for 32,869 (70.8%) of the TACs and gene ontology assignments were determined for 21,471 (46.3%). The new transcriptome assembly was compared with the previously available chickpea transcriptome assemblies as well as to the chickpea genome. Comparative analysis of CaTA v2 against transcriptomes of three legumes - Medicago, soybean and common bean, resulted in 27,771 TACs common to all three legumes indicating strong conservation of genes across legumes. CaTA v2 was also used for identification of simple sequence repeats (SSRs) and intron spanning regions (ISRs) for developing molecular markers. ISRs were identified by aligning TACs to the Medicago genome, and their putative mapping positions at chromosomal level were identified using transcript map of chickpea. Primer pairs were designed for 4,990 ISRs, each representing a single contig for which predicted positions are inferred and distributed across eight linkage groups. A subset of randomly selected ISRs representing all eight chickpea linkage groups were validated on five chickpea genotypes and showed 20% polymorphism with average polymorphic information content (PIC) of 0.27. In summary, the hybrid transcriptome assembly developed and novel markers identified can be used for a variety of applications such as gene discovery, marker-trait association, diversity analysis etc., to advance genetics research and breeding applications in chickpea and other related legumes. C1 [Kudapa, Himabindu; Azam, Sarwar; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Res Program Grain Legumes, Patancheru, Andhra Pradesh, India. [Sharpe, Andrew G.; Li, Rong] Natl Res Council Canada NRC CNRC, Saskatoon, SK, Canada. [Taran, Bunyamin] Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK, Canada. [Deonovic, Benjamin; Cannon, Steven B.] Univ Iowa, Dept Agron, Ames, IA USA. [Cameron, Connor; Farmer, Andrew D.] NCGR, Santa Fe, NM USA. [Cannon, Steven B.] USDA ARS, Corn Insects & Crop Genet Res Unit USDA ARS CICGR, Ames, IA USA. [Varshney, Rajeev K.] CIMMYT, CGIAR Generat Challenge Programme GCP, Mexico City 06600, DF, Mexico. RP Varshney, RK (reprint author), Int Crops Res Inst Semi Arid Trop, Res Program Grain Legumes, Patancheru, Andhra Pradesh, India. EM r.k.varshney@cgiar.org RI Varshney, Rajeev/C-5295-2014 OI Varshney, Rajeev/0000-0002-4562-9131 FU DST-INSPIRE program by Department of Science and Technology, Government of India; Indo-German Science Technology Centre (IGSTC); CGIAR Generation Challenge Programme (GCP); Saskatchewan Agriculture Development Fund (ADF) FX The authors are thankful to the DST-INSPIRE program by Department of Science and Technology, Government of India, Indo-German Science Technology Centre (IGSTC), CGIAR Generation Challenge Programme (GCP) and the Saskatchewan Agriculture Development Fund (ADF) for financial support to undertake part of research presented in this study. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 49 TC 22 Z9 22 U1 0 U2 22 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 23 PY 2014 VL 9 IS 1 AR e86039 DI 10.1371/journal.pone.0086039 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297XI UT WOS:000330288000043 PM 24465857 ER PT J AU Notari, L Riera, DC Sun, R Bohl, JA McLean, LP Madden, KB van Rooijen, N Vanuytsel, T Urban, JF Zhao, AP Shea-Donohue, T AF Notari, Luigi Riera, Diana C. Sun, Rex Bohl, Jennifer A. McLean, Leon P. Madden, Kathleen B. van Rooijen, Nico Vanuytsel, Tim Urban, Joseph F., Jr. Zhao, Aiping Shea-Donohue, Terez TI Role of Macrophages in the Altered Epithelial Function during a Type 2 Immune Response Induced by Enteric Nematode Infection SO PLOS ONE LA English DT Article ID ALTERNATIVELY ACTIVATED MACROPHAGES; BRUSH-BORDER MEMBRANE; RAT SMALL-INTESTINE; HELIGMOSOMOIDES-POLYGYRUS INFECTION; INDUCIBLE FACTOR-I; GLUCOSE-METABOLISM; INSULIN-RESISTANCE; SUGAR ABSORPTION; TASTE RECEPTORS; INNATE IMMUNITY AB Parasitic enteric nematodes induce a type 2 immune response characterized by increased production of Th2 cytokines, IL-4 and IL-13, and recruitment of alternatively activated macrophages ( M2) to the site of infection. Nematode infection is associated with changes in epithelial permeability and inhibition of sodium-linked glucose absorption, but the role of M2 in these effects is unknown. Clodronate-containing liposomes were administered prior to and during nematode infection to deplete macrophages and prevent the development of M2 in response to infection with Nippostrongylus brasiliensis. The inhibition of epithelial glucose absorption that is associated with nematode infection involved a macrophage-dependent reduction in SGLT1 activity, with no change in receptor expression, and a macrophage-independent down-regulation of GLUT2 expression. The reduced transport of glucose into the enterocyte is compensated partially by an up-regulation of the constitutive GLUT1 transporter consistent with stress-induced activation of HIF-1 alpha. Thus, nematode infection results in a "lean" epithelial phenotype that features decreased SGLT1 activity, decreased expression of GLUT2 and an emergent dependence on GLUT1 for glucose uptake into the enterocyte. Macrophages do not play a role in enteric nematode infection-induced changes in epithelial barrier function. There is a greater contribution, however, of paracellular absorption of glucose to supply the energy demands of host resistance. These data provide further evidence of the ability of macrophages to alter glucose metabolism of neighboring cells. C1 [Notari, Luigi; Sun, Rex; Bohl, Jennifer A.; McLean, Leon P.; Zhao, Aiping; Shea-Donohue, Terez] Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. [Notari, Luigi; Sun, Rex; Bohl, Jennifer A.; McLean, Leon P.; Zhao, Aiping; Shea-Donohue, Terez] Univ Maryland, Sch Med, Mucosal Biol Res Ctr, Baltimore, MD 21201 USA. [Riera, Diana C.] Walter Reed Army Med Ctr, Dept Pediat, Washington, DC 20307 USA. [Riera, Diana C.; Madden, Kathleen B.] Uniformed Serv Univ Hlth Sci, Dept Pediat, Bethesda, MD 20814 USA. [van Rooijen, Nico] Vrije Univ Amsterdam, VUMC, Dept Mol Cell Biol, Amsterdam, Netherlands. [Vanuytsel, Tim] Katholieke Univ Leuven, Univ Hosp Gasthuisberg, Translat Res Ctr Gastrointestinal Disorders, Louvain, Belgium. [Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. RP Shea-Donohue, T (reprint author), Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. EM tdonohue@mbrc.maryland.edu OI Urban, Joseph/0000-0002-1590-8869 FU National Institutes of Health [R01-AI/DK49316, DK083418, T32 DK-067872]; U.S. Department of Agriculture CRIS Project [1235-52000-053]; Flanders research foundation (FWO, Fonds Wetenschappelijk Onderzoek) FX This work was supported by National Institutes of Health Grants R01-AI/DK49316 (to T.S.-D.), DK083418 (to A.Z.), T32 DK-067872 (to L. P. M., J.A.B., and R. S.), by U.S. Department of Agriculture CRIS Project 1235-52000-053 (to J.F.U.) and by a grant from the Flanders research foundation (FWO, Fonds Wetenschappelijk Onderzoek) (to T. V.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 66 TC 7 Z9 7 U1 2 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 23 PY 2014 VL 9 IS 1 AR e84763 DI 10.1371/journal.pone.0084763 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297XI UT WOS:000330288000006 PM 24465430 ER PT J AU Pekin, BK Wisdom, MJ Endress, BA Naylor, BJ Parks, CG AF Pekin, Burak K. Wisdom, Michael J. Endress, Bryan A. Naylor, Bridgett J. Parks, Catherine G. TI Ungulate Browsing Maintains Shrub Diversity in the Absence of Episodic Disturbance in Seasonally-Arid Conifer Forest SO PLOS ONE LA English DT Article ID WHITE-TAILED DEER; VEGETATION DYNAMICS; MANAGEMENT; HERBIVORY; REGIMES; FIRE; ECOSYSTEMS; AUSTRALIA; RESPONSES; SURVIVAL AB Ungulates exert a strong influence on the composition and diversity of vegetation communities. However, little is known about how ungulate browsing pressure interacts with episodic disturbances such as fire and stand thinning. We assessed shrub responses to variable browsing pressure by cattle and elk in fuels treated (mechanical removal of fuels followed by prescribed burning) and non-fuels treated forest sites in northeastern Oregon, US. Seven treatment paddocks were established at each site; three with cattle exclusion and low, moderate and high elk browsing pressure, three with elk exclusion and low, moderate and high cattle browsing pressure, and one with both cattle and elk exclusion. The height, cover and number of stems of each shrub species were recorded at multiple plots within each paddock at the time of establishment and six years later. Changes in shrub species composition over the six year period were explored using multivariate analyses. Generalized Linear Mixed Models were used to determine the effect of browsing pressure on the change in shrub diversity and evenness. Vegetation composition in un-browsed paddocks changed more strongly and in different trajectories than in browsed paddocks at sites that were not fuels treated. In fuels treated sites, changes in composition were minimal for un-browsed paddocks. Shrub diversity and evenness decreased strongly in un-browsed paddocks relative to paddocks with low, moderate and high browsing pressure at non-fuels treated sites, but not at fuels treated sites. These results suggest that in the combined absence of fire, mechanical thinning and ungulate browsing, shrub diversity is reduced due to increased dominance by certain shrub species which are otherwise suppressed by ungulates and/or fuels removal. Accordingly, ungulate browsing, even at low intensities, can be used to suppress dominant shrub species and maintain diversity in the absence of episodic disturbance events. C1 [Pekin, Burak K.; Endress, Bryan A.] San Diego Zoo Global, Inst Conservat Res, Div Appl Plant Ecol, Escondido, CA 92027 USA. [Wisdom, Michael J.; Naylor, Bridgett J.; Parks, Catherine G.] US Forest Serv, Pacific NW Res Stn, USDA, La Grande, OR USA. [Endress, Bryan A.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. RP Pekin, BK (reprint author), San Diego Zoo Global, Inst Conservat Res, Div Appl Plant Ecol, Escondido, CA 92027 USA. EM bpekin@sandiegozoo.org FU United States Department of Agriculture (USDA) Forest Service Pacific Northwest Research Station, Oregon State University; San Diego Zoo's Institute for Conservation Research FX Research was funded by the United States Department of Agriculture (USDA) Forest Service Pacific Northwest Research Station, Oregon State University, and San Diego Zoo's Institute for Conservation Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 7 Z9 8 U1 2 U2 28 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 23 PY 2014 VL 9 IS 1 AR e86288 DI 10.1371/journal.pone.0086288 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297XI UT WOS:000330288000078 PM 24466006 ER PT J AU Poothong, S Reed, BM AF Poothong, Sukalya Reed, Barbara M. TI Modeling the effects of mineral nutrition for improving growth and development of micropropagated red raspberries SO SCIENTIA HORTICULTURAE LA English DT Article DE In vitro culture; Mineral nutrition; Response surface methodology; Rubus; Red raspberry ID TOBACCO TISSUE CULTURES; IN-VITRO; SHOOT PROLIFERATION; INVITRO; PLANT; MULTIPLICATION; OPTIMIZATION; REGENERATION; ROOTSTOCK; MEDIA AB In vitro propagation is important for rapid multiplication of a wide range of nursery crops, including red raspberries. The genetic variation of the many red raspberry cultivars makes it difficult to use one growth medium for all. Although some cultivars grow well on Murashige and Skoog (1962) medium (MS), others display stunting, hyperhydricity, discoloration, callus, leaf spots, or necrosis. This study used response surface methodology (RSM) to determine the effects of MS mineral salts on red raspberry growth and which of these mineral salts are critical for improving growth. In vitro growth of five red raspberry cultivars was determined by varying five factors that included NH4NO3, KNO3, mesos salts (CaCl2, KH2PO4 and MgSO4), minor elements (Zn-Mn-Cu-Co-Mo-B-I), and EDTA-chelated iron. The effects of these five factors on plant quality, multiplication, shoot length, leaf size, leaf area, leaf color, callus and leaf spots were determined. The effects varied by cultivar for some characteristics, but all cultivars had improved growth or appearance on some experimental treatments when compared to MS medium. Increased mesos was the most significant factor associated with plant quality, multiplication and shoot length in all cultivars. Increasing iron above MS levels decreased quality in all cultivars except 'Willamette'. Decreased KNO3 with increased mesos and low iron were required to improve shoot multiplication. Increased NH4NO3 resulted in greater shoot elongation only in 'Willamette'. Determining the driving mineral factors is the first step in improved medium formulations for micropropagated red raspberries. (C) 2013 Elsevier B.V. All rights reserved. C1 [Poothong, Sukalya] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Reed, Barbara M.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. RP Poothong, S (reprint author), Oregon State Univ, Dept Hort, 4017 Agr & Life Sci Bldg, Corvallis, OR 97331 USA. EM sukalya_p@hotmail.com OI Reed, Barbara/0000-0003-0079-8473 FU U.S. Department of Agriculture, Agricultural Research Service CRIS project [5358-21000-033D]; Royal Thai Government Scholarship FX This project was supported by funds from the U.S. Department of Agriculture, Agricultural Research Service CRIS project 5358-21000-033D. Sukalya Poothong acknowledges the financial support of a Royal Thai Government Scholarship. NR 34 TC 9 Z9 10 U1 3 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JAN 22 PY 2014 VL 165 BP 132 EP 141 DI 10.1016/j.scienta.2013.10.040 PG 10 WC Horticulture SC Agriculture GA AB8QN UT WOS:000332055200018 ER PT J AU Park, SH Burchi, G Roh, MS Joung, YH AF Park, Se Hee Burchi, Gianluca Roh, Mark S. Joung, Young Hee TI Characterization of Kolkwitzia amabilis accessions based on flowering and molecular markers SO SCIENTIA HORTICULTURAE LA English DT Article DE Germplasm repository; Haplotype; ITS 1,2 region; Morphology; Molecular markers; RAPD ID INTERNAL TRANSCRIBED SPACERS; PHYLOGENETIC INFERENCE; CHLOROPLAST DNA; RIBOSOMAL DNA; SNPS; PRIMERS; REPEAT; ORIGIN; KOREA; RAPD AB Accessions of Kolkwitzia amabilis Graebn of wild origin with collection information and cultivated sources with or without collection information were grown under the same environments to observe the differences in morphology and flowering time. The morphological variation was further documented by analyzing the nuclear internal transcribed spacers 1, 2 in ribosomal RNA gene (ITS 1, 2 region) and randomly amplified polymorphic DNA (RAPD) markers. US National Arboretum accession NA64889 of wild origin with collection information (known wild origin) flowered earlier than cultivated sources with (cultivars of known wild origin) or without (cultivars of unknown wild origin) collection information for two years. However, significant variations were observed in sequences of the polymerase chain reaction (PCR) products of all clones of all accessions with the exception of AA20447C. Four out of five clones of NA64889 showing haplotype H were together in the same sub-clusters separated from other accessions. We conclude that NA64889 of known wild origin that flowers early was different from other accessions of cultivated sources of known wild origin (AA816-84A) or of unknown wild origin (AA20447 and AA181-2005). Moreover, the analysis performed on the on the ITS 1,2 region sequence and RAPD markers were shown that all accessions of cultivated origin, analyzed in this study, are derived from germplasm of known wild origin (AA816-84A). (C) 2013 Elsevier B.V. All rights reserved. C1 [Park, Se Hee; Joung, Young Hee] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea. [Burchi, Gianluca] Consiglio Ric Sperimentaz Agr, CRA VIV Landscaping Plants & Nursery Res Unit, I-51012 Pescia, PT, Italy. [Roh, Mark S.] USDA ARS, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. RP Joung, YH (reprint author), Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea. EM yhjoung@chonnam.ac.kr FU Next-Generation BioGreen 21 Program, Rural Development Administration, Korea [PJ00956803] FX This research is supported, in part, by a grant from the Next-Generation BioGreen 21 Program, Rural Development Administration, Korea to Y. H. Joung (PJ00956803). Appreciation is expressed to Jack Alexander and Kyle Port, Arnold Arboretum, for providing plant materials and collection information. Authors thank Dr. R. H. Lawson for constructive suggestions and comments to improve the manuscript. NR 26 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JAN 22 PY 2014 VL 165 BP 190 EP 195 DI 10.1016/j.scienta.2013.11.003 PG 6 WC Horticulture SC Agriculture GA AB8QN UT WOS:000332055200026 ER PT J AU Vazquez-Cintron, EJ Vakulenko, M Band, PA Stanker, LH Johnson, EA Ichtchenko, K AF Vazquez-Cintron, Edwin J. Vakulenko, Maksim Band, Philip A. Stanker, Larry H. Johnson, Eric A. Ichtchenko, Konstantin TI Atoxic Derivative of Botulinum Neurotoxin A as a Prototype Molecular Vehicle for Targeted Delivery to the Neuronal Cytoplasm SO PLOS ONE LA English DT Article ID LIGHT-CHAIN; PROTEIN; TOXIN; TRANSLOCATION; CELLS; MEMBRANES; SEQUENCE; ANTIBODY; SNAP-25; BINDING AB We have previously described genetic constructs and expression systems that enable facile production of recombinant derivatives of botulinum neurotoxins (BoNTs) that retain the structural and trafficking properties of wt BoNTs. In this report we describe the properties of one such derivative, BoNT/A ad, which was rendered atoxic by introducing two amino acid mutations to the light chain (LC) of wt BoNT/A, and which is being developed as a molecular vehicle for delivering drugs to the neuronal cytoplasm. The neuronal binding, internalization, and intracellular trafficking of BoNT/A ad in primary hippocampal cultures was evaluated using three complimentary techniques: flow cytometry, immunohistochemistry, and Western blotting. Neuronal binding of BoNT ad was significantly increased when neurons were incubated in depolarizing medium. Flow cytometry demonstrated that BoNT/A ad internalized into neurons but not glia. After 24 hours, the majority of the neuron-bound BoNT/A ad became internalized, as determined by its resistance to pronase E-induced proteolytic degradation of proteins associated with the plasma membrane of intact cells. Significant amounts of the atoxic LC accumulated in a Triton X-100-extractable fraction of the neurons, and persisted as such for at least 11 days with no evidence of degradation. Immunocytochemical analysis demonstrated that the LC of BoNT/A ad was translocated to the neuronal cytoplasm after uptake and was specifically targeted to SNARE proteins. The atoxic LC consistently co-localized with synaptic markers SNAP-25 and VAMP-2, but was rarely co-localized with markers for early or late endosomes. These data demonstrate that BoNT/A ad mimics the trafficking properties of wt BoNT/A, confirming that our platform for designing and expressing BoNT derivatives provides an accessible system for elucidating the molecular details of BoNT trafficking, and can potentially be used to address multiple medical and biodefense needs. C1 [Vazquez-Cintron, Edwin J.; Vakulenko, Maksim; Band, Philip A.; Ichtchenko, Konstantin] New York Univ Sch Med, Dept Biochem & Mol Pharmacol, New York, NY 10012 USA. [Band, Philip A.] New York Univ Hosp Joint Dis, Dept Orthopaed Surg, New York, NY USA. [Stanker, Larry H.] ARS, USDA, Albany, CA USA. [Johnson, Eric A.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. RP Ichtchenko, K (reprint author), New York Univ Sch Med, Dept Biochem & Mol Pharmacol, New York, NY 10012 USA. EM ichtck01@med.nyu.edu OI Ichtchenko, Konstantin/0000-0002-2198-1325 FU National Institute Of Allergy And Infectious Diseases [R01-AI093504]; USDA CRIS [5325-42000-043-00D]; National Cancer Institute [5P30CA016087-32] FX Support for this work was provided by the National Institute Of Allergy And Infectious Diseases R01-AI093504 to KI and EAJ and by USDA CRIS Project 5325-42000-043-00D to LHS. The NYU SoM Flow Cytometry Core is supported in part by grant 5P30CA016087-32 from the National Cancer Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 8 Z9 8 U1 0 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 22 PY 2014 VL 9 IS 1 AR e85517 DI 10.1371/journal.pone.0085517 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297VL UT WOS:000330283100045 PM 24465585 ER PT J AU Lemoine, M Young, MK McKelvey, KS Eby, L Pilgrim, KL Schwartz, MK AF Lemoine, Michael Young, Michael K. McKelvey, Kevin S. Eby, Lisa Pilgrim, Kristine L. Schwartz, Michael K. TI Cottus schitsuumsh, a new species of sculpin (Scorpaeniformes: Cottidae) in the Columbia River basin, Idaho-Montana, USA SO ZOOTAXA LA English DT Article DE Cedar Sculpin; COI; Couer d'Alene River; DNA barcode; microsatellite; Shorthead Sculpin; St. Joe River ID MOTTLED SCULPIN; NORTH-AMERICA; DNA BARCODES; TELEOSTEI; TAXONOMY; FISHES; POPULATION; DIVERSITY; INFERENCE; COGNATUS AB Fishes of the genus Cottus have long been taxonomically challenging because of morphological similarities among species and their tendency to hybridize, and a number of undescribed species may remain in this genus. We used a combination of genetic and morphological methods to delineate and describe Cottus schitsuumsh, Cedar Sculpin, a new species, from the upper Columbia River basin, Idaho-Montana, USA. Although historically confused with the Shorthead Sculpin (C. confusus), the genetic distance between C. schitsuumsh and C. confusus (4.84-6.29%) suggests these species are distant relatives. Moreover, the two species can be differentiated on the basis of lateral-line pores on the caudal peduncle, head width, and interpelvic width. Cottus schitsuumsh is also distinct from all other Cottus in this region in having a single small, skin-covered, preopercular spine. Haplotypes of mtDNA cytochrome oxidase c subunit 1 of C. schitsuumsh differed from all other members of the genus at three positions, had interspecific genetic distances typical for congeneric fishes (1.61-2.74% to nearest neighbors), and were monophyletic in maximum-likelihood trees. Microsatellite analyses confirmed these taxonomic groupings for species potentially sympatric with C. schitsuumsh and that fish used in morphological comparisons were unlikely to be introgressed. Its irregular distribution, in the Spokane River basin in Idaho and portions of the Clark Fork River basin in Montana, may have resulted from human-assisted translocation. C1 [Lemoine, Michael; Eby, Lisa] Univ Montana, Wildlife Biol Program, Missoula, MT 59812 USA. [Young, Michael K.; McKelvey, Kevin S.; Pilgrim, Kristine L.; Schwartz, Michael K.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Lemoine, M (reprint author), Univ Montana, Wildlife Biol Program, Missoula, MT 59812 USA. EM michael1.lemoine@umconnect.umt.edu RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 FU U.S. Forest Service (Region 1 and the Rocky Mountain Research Station); University of Montana FX We thank the elders of the Coeur d'Alene Tribe for assistance with choosing a species name. We thank Montana Fish, Wildlife and Parks, the Washington Departments of Ecology and Fish and Wildlife, Bart Gamett (Salmon-Challis National Forest), and Ernest Keeley (Idaho State University) for providing additional samples. Don Zaroban granted access to collections at the Orma J. Smith Museum (College of Idaho; CIDA) and assisted with morphological assessments. Grace Malato assisted with the morphological comparisons, Dan Bingham with the genetic analyses, and Lisa Holsinger with the range map. We especially thank Emily Harrington at E.H. Illustration (Missoula, MT) for the illustrations in Figures 4, 6 and 7, and Zachary Randall and Larry Page with their photographic expertise and producing Figure 5. Portions of the field sampling were performed under scientific collection permits issued to Michael Young by the Idaho Department of Fish and Game and Montana Fish, Wildlife and Parks. This work was funded by the U.S. Forest Service (Region 1 and the Rocky Mountain Research Station) and the University of Montana. NR 45 TC 2 Z9 2 U1 2 U2 17 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JAN 22 PY 2014 VL 3755 IS 3 BP 241 EP 258 PG 18 WC Zoology SC Zoology GA 294DR UT WOS:000330023100003 PM 24869819 ER PT J AU Carbonero, F Oakley, BB Purdy, KJ AF Carbonero, Franck Oakley, Brian B. Purdy, Kevin J. TI Metabolic Flexibility as a Major Predictor of Spatial Distribution in Microbial Communities SO PLOS ONE LA English DT Article ID ESTUARINE GRADIENT; ECOLOGICAL SPECIALIZATION; GENOTYPIC DISTRIBUTION; BACTERIAL COMMUNITIES; MODEL MICROORGANISM; SULFATE REDUCTION; ORGANIC-MATTER; RIBOSOMAL-RNA; UK ESTUARY; DIVERSITY AB A better understand the ecology of microbes and their role in the global ecosystem could be achieved if traditional ecological theories can be applied to microbes. In ecology organisms are defined as specialists or generalists according to the breadth of their niche. Spatial distribution is often used as a proxy measure of niche breadth; generalists have broad niches and a wide spatial distribution and specialists a narrow niche and spatial distribution. Previous studies suggest that microbial distribution patterns are contrary to this idea; a microbial generalist genus (Desulfobulbus) has a limited spatial distribution while a specialist genus (Methanosaeta) has a cosmopolitan distribution. Therefore, we hypothesise that this counter-intuitive distribution within generalist and specialist microbial genera is a common microbial characteristic. Using molecular fingerprinting the distribution of four microbial genera, two generalists, Desulfobulbus and the methanogenic archaea Methanosarcina, and two specialists, Methanosaeta and the sulfate-reducing bacteria Desulfobacter were analysed in sediment samples from along a UK estuary. Detected genotypes of both generalist genera showed a distinct spatial distribution, significantly correlated with geographic distance between sites. Genotypes of both specialist genera showed no significant differential spatial distribution. These data support the hypothesis that the spatial distribution of specialist and generalist microbes does not match that seen with specialist and generalist large organisms. It may be that generalist microbes, while having a wider potential niche, are constrained, possibly by intrageneric competition, to exploit only a small part of that potential niche while specialists, with far fewer constraints to their niche, are more capable of filling their potential niche more effectively, perhaps by avoiding intrageneric competition. We suggest that these counter-intuitive distribution patterns may be a common feature of microbes in general and represent a distinct microbial principle in ecology, which is a real challenge if we are to develop a truly inclusive ecology. C1 [Carbonero, Franck; Oakley, Brian B.; Purdy, Kevin J.] Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England. [Carbonero, Franck] Univ Arkansas, Dept Food Sci, Fayetteville, AR 72703 USA. [Oakley, Brian B.] ARS, USDA, Richard B Russell Res Ctr, Athens, GA USA. RP Purdy, KJ (reprint author), Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England. EM k.purdy@warwick.ac.uk RI Purdy, Kevin/A-1900-2009 OI Purdy, Kevin/0000-0002-2997-9342 FU EU Commission [MEXT-CT-2005-024112] FX This study was performed as part of an EU Commission funded Marie Curie Excellence Grant for Teams, MicroComXT (MEXT-CT-2005-024112) to KJP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 46 TC 7 Z9 7 U1 2 U2 42 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 21 PY 2014 VL 9 IS 1 AR e85105 DI 10.1371/journal.pone.0085105 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297GT UT WOS:000330244500037 PM 24465487 ER PT J AU Sharma, VK Casey, TA AF Sharma, Vijay K. Casey, Thomas A. TI Determining the Relative Contribution and Hierarchy of hha and qseBC in the Regulation of Flagellar Motility of Escherichia coli O157:H7 SO PLOS ONE LA English DT Article ID ENTERICA SEROVAR TYPHIMURIUM; ENTEROCYTE EFFACEMENT; H-NS; VIRULENCE FACTORS; TRANSCRIPTIONAL REGULATION; PATHOGENICITY ISLAND; TERMINAL RECTUM; SENSOR KINASE; WEANED CALVES; III SECRETION AB In recent studies, we demonstrated that a deletion of hha caused increased secretion of locus of enterocyte encoded adherence proteins and reduced motility of enterohemorrhagic Escherichia coli (EHEC) O157:H7. In addition to the importance of hha in positive regulation of motility, a two-component quorum sensing pathway encoded by the qseBC genes has been shown to activate bacterial motility in response to mammalian stress hormones epinephrine and norepinephrine as well as bacterially produced autoinducer-3. In this study, we compared regulatory contribution and hierarchy of hha, a member of the Hha/YmoA family of nucleoid-associated proteins, to that of qseBC in the expression of EHEC O157:H7 motility. Since norepinephrine affects motility of EHEC O157:H7 through a qseBC-encoded two-component quorum sensing signaling, we also determined whether the hha-mediated regulation of motility is affected by norepinephrine and whether this effect is qseBC dependent. We used single (Delta hha or Delta qseC) and double (Delta hha Delta qseC) deletion mutants to show that hha exerts a greater positive regulatory effect in comparison to qseBC on the expression of motility by EHEC O157:H7. We also show that Hha is hierarchically superior in transcriptional regulation of motility than QseBC because transcription of qseC was significantly reduced in the hha deletion mutant compared to that in the parental and the hha-complemented mutant strains. These results suggest that hha regulates motility of EHEC O157:H7 directly as well as indirectly by controlling the transcription of qseBC. C1 [Sharma, Vijay K.; Casey, Thomas A.] ARS, Food Safety & Enter Pathogens Res Unit, USDA, Natl Anim Dis Ctr, Ames, IA 50011 USA. RP Sharma, VK (reprint author), ARS, Food Safety & Enter Pathogens Res Unit, USDA, Natl Anim Dis Ctr, Ames, IA 50011 USA. EM vijay.sharma@ars.usda.gov FU USDA-ARS FX USDA-ARS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 57 TC 3 Z9 3 U1 2 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 21 PY 2014 VL 9 IS 1 AR e85866 DI 10.1371/journal.pone.0085866 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297GT UT WOS:000330244500134 PM 24465756 ER PT J AU Wu, FQ Price, BW Haider, W Seufferheld, G Nelson, R Hanzawa, Y AF Wu, Faqiang Price, Brian William Haider, Waseem Seufferheld, Gabriela Nelson, Randall Hanzawa, Yoshie TI Functional and Evolutionary Characterization of the CONSTANS Gene Family in Short-Day Photoperiodic Flowering in Soybean SO PLOS ONE LA English DT Article ID MAP-BASED CLONING; TIME GENE; ARABIDOPSIS-THALIANA; GLYCINE-MAX; LOCUS-T; EXPRESSION; MATURITY; GENOME; SEQUENCE; HOMOLOGS AB CONSTANS (CO) plays a central role in photoperiodic flowering control of plants. However, much remains unknown about the function of the CO gene family in soybean and the molecular mechanisms underlying short-day photoperiodic flowering of soybean. We identified 26 CO homologs (GmCOLs) in the soybean genome, many of them previously unreported. Phylogenic analysis classified GmCOLs into three clades conserved among flowering plants. Two homeologous pairs in Clade I, GmCOL1a/GmCOL1b and GmCOL2a/GmCOL2b, showed the highest sequence similarity to Arabidopsis CO. The mRNA abundance of GmCOL1a and GmCOL1b exhibited a strong diurnal rhythm under flowering-inductive short days and peaked at dawn, which coincided with the rise of GmFT5a expression. In contrast, the mRNA abundance of GmCOL2a and GmCOL2b was extremely low. Our transgenic study demonstrated that GmCOL1a, GmCOL1b, GmCOL2a and GmCOL2b fully complemented the late flowering effect of the co-1 mutant in Arabidopsis. Together, these results indicate that GmCOL1a and GmCOL1b are potential inducers of flowering in soybean. Our data also indicate rapid regulatory divergence between GmCOL1a/GmCOL1b and GmCOL2a/GmCOL2b but conservation of their protein function. Dynamic evolution of GmCOL regulatory mechanisms may underlie the evolution of photoperiodic signaling in soybean. C1 [Wu, Faqiang; Price, Brian William; Haider, Waseem; Seufferheld, Gabriela; Hanzawa, Yoshie] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Nelson, Randall] Univ Illinois, Dept Crop Sci, USDA Agr Res Serv, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL USA. RP Hanzawa, Y (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM yhanzawa@illinois.edu FU Agriculture and Food Research Initiative Competitive Grants Program from the USDA National Institute of Food and Agriculture [USDA-NIFA2011-00078] FX This project is supported by the Agriculture and Food Research Initiative Competitive Grants Program from the USDA National Institute of Food and Agriculture (USDA-NIFA2011-00078). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 59 TC 21 Z9 22 U1 4 U2 20 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 21 PY 2014 VL 9 IS 1 AR e85754 DI 10.1371/journal.pone.0085754 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297GT UT WOS:000330244500105 PM 24465684 ER PT J AU Yu, LX Parthasarathy, MV AF Yu, Long-Xi Parthasarathy, Mandayam V. TI Molecular and Cellular Characterization of the Tomato Pollen Profilin, LePro1 SO PLOS ONE LA English DT Article ID PHOSPHOLIPASE-C; TUBE GROWTH; ACTIN; ISOFORMS; CYTOSKELETON; ALLERGEN; PLANTS; LOCALIZATION; ARABIDOPSIS; GERMINATION AB Profilin is an actin-binding protein involved in the dynamic turnover and restructuring of the actin cytoskeleton in all eukaryotic cells. We previously cloned a profilin gene, designated as LePro1 from tomato pollen. To understand its biological role, in the present study, we investigated the temporal and spatial expression of LePro1 during pollen development and found that the transcript was only detected at late stages during microsporogenesis and pollen maturation. Using antisense RNA, we successfully knocked down the expression of LePro1 in tomato plants using stable transformation, and obtained two antisense lines, A2 and A3 showing significant down-regulation of LePro1 in pollen resulting in poor pollen germination and abnormal pollen tube growth. A disorganized F-actin distribution was observed in the antisense pollen. Downregulation of LePro1 also appeared to affect hydration of pollen deposited on the stigma and arrested pollen tube elongation in the style, thereby affecting fertilization. Our results suggest that LePro1 in conjunction with perhaps other cytoskeletal proteins, plays a regulatory role in the proper organization of F-actin in tomato pollen tubes through promoting actin assembly. Down-regulation of LePro1 leads to interruption of actin assembly and disorganization of the actin cytoskeleton thus arresting pollen tube growth. Based on the present and previous studies, it is likely that a single transcript of profilin gives rise to multiple forms displaying multifunctionality in tomato pollen. C1 [Yu, Long-Xi; Parthasarathy, Mandayam V.] Cornell Univ, Dept Plant Biol, Ithaca, NY USA. RP Yu, LX (reprint author), ARS, USDA, Vegetable & Forage Crop Res Unit, Prosser, WA 99350 USA. EM longxi.yu@ars.usda.gov FU National Research Initiative competitive grants program/United States Department of Agriculture [94-37304] FX This work was supported by a National Research Initiative competitive grants program/United States Department of Agriculture 94-37304 to M.V.P. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 40 TC 0 Z9 0 U1 0 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 21 PY 2014 VL 9 IS 1 AR e86505 DI 10.1371/journal.pone.0086505 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297GT UT WOS:000330244500249 PM 24466125 ER PT J AU Bosworth, B Waldbieser, G AF Bosworth, Brian Waldbieser, Geoff TI Reprint of: General and specific combining ability of male blue catfish (Ictalurus furcatus) and female channel catfish (Ictalurus punctatus) for growth and carcass yield of their F-1 hybrid progeny SO AQUACULTURE LA English DT Article DE Hybrid; Catfish; Combining ability; Performance ID GENOTYPE-ENVIRONMENT INTERACTIONS; GENETIC-PARAMETERS; CROSSBRED PERFORMANCE; ECONOMIC TRAITS; RAINBOW-TROUT; HERITABILITY; L.; SILURIFORMES; ASSIGNMENT; PARENTAGE AB U.S. aquaculture production of channel catfish female (Ictalurus punctatus) x blue catfish male (Ictalurus furcatus) F-1 hybrids has increased substantially due to the hybrid's improved growth, survival, carcass yield and ease of harvest compared to the more commonly farmed channel catfish. However, information on the genetic architecture underlying phenotypic variation in the hybrid which is needed to develop an efficient genetic improvement program is lacking. Progeny from two separate factorial matings between male blue catfish and female channel catfish (ten males x seven females and twelve males x five females, respectively) were grown communally in earthen ponds and measured for carcass yield and weight at approximately 520 days post-hatch. Parentage of progeny was assigned by inheritance of microsatellite genotypes. Progeny from 118 full-sib families, within 12 maternal half-sib and 22 paternal half-sib families, were measured for harvest weight (n = 1288) and carcass yield (n = 1101). Variance component estimates associated with dams (dam general combining ability and heritabilities based on dam half-sib families) were high, variance associated with sires (sire general combining ability and heritabilities based on sire half-sib families) were intermediate, and variance components associated with the dam x sire interactions (specific combining ability) were low for carcass yield and harvest weight. Data indicate that the genetic variance for carcass yield and harvest weight of F-1 hybrids was primarily additive. Selection for improved carcass yield and growth based on additive genetic merit of blue catfish male and channel catfish female parents should be effective for improving performance of hybrid progeny. Published by Elsevier B.V. C1 [Bosworth, Brian; Waldbieser, Geoff] ARS, Catfish Genet Res Unit, USDA, Stoneville, MS 38776 USA. RP Bosworth, B (reprint author), ARS, Catfish Genet Res Unit, USDA, POB 38, Stoneville, MS 38776 USA. EM brian.bosworth@ars.usda.gov NR 49 TC 1 Z9 1 U1 3 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD JAN 20 PY 2014 VL 420 SU 1 BP S29 EP S35 DI 10.1016/j.aquaculture.2014.01.017 PG 7 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AE5DY UT WOS:000334009200007 ER PT J AU Pitino, M Hoffman, MT Zhou, LJ Hall, DG Stocks, IC Duan, YP AF Pitino, Marco Hoffman, Michele T. Zhou, Lijuan Hall, David G. Stocks, Ian C. Duan, Yongping TI The Phloem-Sap Feeding Mealybug (Ferrisia virgata) Carries 'Candidatus Liberibacter asiaticus' Populations That Do Not Cause Disease in Host Plants SO PLOS ONE LA English DT Article ID REAL-TIME PCR; CITRUS HUANGLONGBING DISEASE; INTRAGENIC TANDEM-REPEATS; HEMIPTERA PSYLLIDAE; DIAPHORINA-CITRI; GREENING DISEASE; PROPHAGE GENES; SWEET ORANGE; PAULO STATE; VECTOR AB 'Candidatus Liberibacter asiaticus' (Las) is the primary causal agent of huanglongbing (HLB), the most devastating disease of citrus worldwide. There are three known insect vectors of the HLB-associated bacteria, and all are members of the Hemiptera: Diaphorina citri (Psyllidae), Trioza erytreae (Triozidae), and Cacopsylla (Psylla) citrisuga (Psyllidae). In this study, we found that another hemipteran, the striped mealybug Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae), was able to acquire and retain Las bacteria. The bacterial titers were positively correlated with the feeding acquisition time on Las-infected leaf discs, with a two-weeks feeding period resulting in Ct values ranging from 23.1 to 36.1 (8.24x10(7) to 1.07x10(4) Las cells per mealybug). We further discovered that the prophage/phage populations of Las in the mealybugs were different from those of Las in psyllids based on Las prophage-specific molecular markers: infected psyllids harbored the Las populations with prophage/phage FP1 and FP2, while infected mealybugs carried the Las populations with the iFP3 being the dominant prophage/phage. As in the psyllids, Las bacteria were shown to move through the insect gut wall to the salivary glands after being ingested by the mealybug based on a time-course quantitative polymerase chain reaction (qPCR) assay of the dissected digestive systems. However, Las populations transmitted by the mealybugs did not cause disease in host plants. This is the first evidence of genetic difference among Las populations harbored by different insect vectors and difference among Las populations with respect to whether or not they cause disease in host plants. C1 [Pitino, Marco; Hoffman, Michele T.; Zhou, Lijuan; Hall, David G.; Duan, Yongping] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. [Zhou, Lijuan] Univ Florida, Inst Food & Agr Sci, Indian River Res & Educ Ctr, Ft Pierce, FL USA. [Stocks, Ian C.] Florida Dept Agr & Consumer Serv, Drug Policy Inst, Gainesville, FL USA. RP Duan, YP (reprint author), ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. EM yongping.duan@ars.usda.gov FU United States Department of Agriculture; Florida Department of Agriculture and Consumer Services-Specialty Crop Block Grant [18023] FX Funding provided by United States Department of Agriculture and Florida Department of Agriculture and Consumer Services-Specialty Crop Block Grant #18023. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 3 Z9 3 U1 2 U2 26 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 20 PY 2014 VL 9 IS 1 AR e85503 DI 10.1371/journal.pone.0085503 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297FH UT WOS:000330240500052 ER PT J AU Vega, FE Simpkins, A Bauchan, G Infante, F Kramer, M Land, MF AF Vega, Fernando E. Simpkins, Ann Bauchan, Gary Infante, Francisco Kramer, Matthew Land, Michael F. TI On the Eyes of Male Coffee Berry Borers as Rudimentary Organs SO PLOS ONE LA English DT Article ID COLEOPTERA-CURCULIONIDAE SCOLYTINAE; XYLEBORUS-FERRUGINEUS; COMPOUND EYE; BEETLE; ULTRASTRUCTURE; INSECTS AB The coffee berry borer, Hypothenemus hampei, is the most damaging insect pest of coffee worldwide. Like males in other species in the genus, male coffee berry borers have a lower number of facets in the compound eyes than females. The rudimentary eyes in male coffee berry borers could be an evolutionary response to their cryptic life habit, whereby they are born inside a coffee berry and never leave the berry. The main objective of the study was to determine if the differences in the number of facets translates into differences in visual acuity. We used low-temperature scanning electron microscopy to visualize and quantify the number of facets in the compound eyes. There was a significantly lower (p<0.0001) number of facets in males (19.1 +/- 4.10) than in females (127.5 +/- 3.88). To assess visual acuity, we conducted optomotor response experiments, which indicate that females respond to movement, while males did not respond under the conditions tested. The coffee berry borer is an example of an insect whereby disuse of an organ has led to a rudimentary compound eye. This is the first study that has experimentally tested responses to movement in bark beetles. C1 [Vega, Fernando E.; Simpkins, Ann] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD USA. [Bauchan, Gary] ARS, Electron & Confocal Microscopy Unit, USDA, Beltsville, MD USA. [Infante, Francisco] El Colegio Frontera Sur, Tapachula, Chiapas, Mexico. [Kramer, Matthew] ARS, Biometr Consulting Serv, USDA, Beltsville, MD USA. [Land, Michael F.] Univ Sussex, Sch Life Sci, Brighton, E Sussex, England. RP Vega, FE (reprint author), ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD USA. EM Fernando.Vega@ars.usda.gov OI Infante, Francisco/0000-0002-7419-7606; Vega, Fernando E./0000-0001-8103-5640 FU U.S. Department of Agriculture FX This work was supported by U.S. Department of Agriculture core funds. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 24 TC 7 Z9 8 U1 0 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 20 PY 2014 VL 9 IS 1 AR e85860 DI 10.1371/journal.pone.0085860 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297FH UT WOS:000330240500081 PM 24465752 ER PT J AU Velazquez-Salinas, L Pauszek, SJ Zarate, S Basurto-Alcantara, FJ Verdugo-Rodriguez, A Perez, AM Rodriguez, LL AF Velazquez-Salinas, Lauro Pauszek, Steven J. Zarate, Selene Basurto-Alcantara, Francisco J. Verdugo-Rodriguez, Antonio Perez, Andres M. Rodriguez, Luis L. TI Phylogeographic characteristics of vesicular stomatitis New Jersey viruses circulating in Mexico from 2005 to 2011 and their relationship to epidemics in the United States SO VIROLOGY LA English DT Article DE Vesicular stomatitis virus; Epidemiology; Spatial clustering; Phylogeny; Phylodynamics; Mexico; United States; Outbreak ID EQUINE ENCEPHALITIS-VIRUS; E2 ENVELOPE GLYCOPROTEIN; EVOLUTION; REEMERGENCE; EMERGENCE; OUTBREAKS; SEQUENCE; VECTORS; STRAINS AB We analyzed the phylogenetic and time-space relationships (phylodynamics) of 181 isolates of vesicular stomatitis New Jersey virus (VSNJV) causing disease in Mexico and the United States (US) from 2005 through 2012. We detail the emergence of a genetic lineage in southern Mexico causing outbreaks in central Mexico spreading into northern Mexico and eventually into the US. That emerging lineage showed higher nucleotide sequence identity (99.5%) than that observed for multiple lineages circulating concurrently in southern Mexico (96.8%). Additionally, we identified 58 isolates from Mexico that, unlike previous isolates from Mexico, grouped with northern Central America clade II viruses. This study provides the first direct evidence for the emergence and northward migration of a specific VSNJV genetic lineage from endemic areas in Mexico causing VS outbreaks in the US. In addition we document the emergence of a Central American VSNJV genetic lineage moving northward and causing outbreaks in central Mexico. Published by Elsevier Inc. C1 [Velazquez-Salinas, Lauro; Pauszek, Steven J.; Rodriguez, Luis L.] USDA ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, Greenport, NY 11944 USA. [Zarate, Selene] Autonomous Univ Mexico, Dept Genom Sci, Mexico City, DF, Mexico. [Basurto-Alcantara, Francisco J.; Verdugo-Rodriguez, Antonio] Univ Nacl Autonoma Mexico, Coll Vet Med & Anim Sci, Mexico City, DF, Mexico. [Perez, Andres M.] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA. [Velazquez-Salinas, Lauro] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. RP Rodriguez, LL (reprint author), USDA ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, POB 848, Greenport, NY 11944 USA. EM luis.rodriguez@ars.usda.gov NR 32 TC 1 Z9 2 U1 1 U2 6 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD JAN 20 PY 2014 VL 449 BP 17 EP 24 DI 10.1016/j.virol.2013.10.025 PG 8 WC Virology SC Virology GA 295CL UT WOS:000330094100002 PM 24418533 ER PT J AU Nyasembe, VO Teal, PEA Sawa, P Tumlinson, JH Borgemeister, C Torto, B AF Nyasembe, Vincent O. Teal, Peter E. A. Sawa, Patrick Tumlinson, James H. Borgemeister, Christian Torto, Baldwyn TI Plasmodium falciparum Infection Increases Anopheles gambiae Attraction to Nectar Sources and Sugar Uptake SO CURRENT BIOLOGY LA English DT Article ID MALARIA VECTOR; AEDES-AEGYPTI; YOELII-NIGERIENSIS; MOSQUITOS; STEPHENSI; HOST; FECUNDITY; PARASITES; SURVIVAL; MANIPULATION AB Plasmodium parasites are known to manipulate the behavior of their vectors so as to enhance transmission [1-4]. From an evolutionary standpoint, behavior manipulation by the parasite should expose the vector to limited risk of early mortality while ensuring sufficient energy supply for both it and the vector [5, 6]. However, it is unknown whether this vector manipulation also affects vector-plant interaction and sugar uptake. Here, we show that the attraction of Anopheles gambiae s.s. to plant odors increased by 30% and 24% after infection with the oocyst and sporozoite stages of Plasmodium falciparum, respectively, while probing activity increased by 77% and 80%, respectively, when the vectors were infected with the two stages of the parasite. Our data also reveal an increased sugar uptake at the oocyst stage that decreased at the sporozoite stage of infection compared to uninfected An. gambiae, with depletion of lipid reserves at the sporozoite stage. These results point to a possible physiological adjustment by An. gambiae to P. falciparum infection or behavior manipulation of An. gambiae by P. falciparum to enhance transmission. We conclude that the nectar-seeking behavior of P. falciparum-infected An. gambiae appears to be modified in a manner governed by the vector's fight for survival and the parasite's need to advance its transmission. C1 [Nyasembe, Vincent O.; Sawa, Patrick; Borgemeister, Christian; Torto, Baldwyn] Int Ctr Insect Physiol & Ecol, Nairobi 00100, Kenya. [Teal, Peter E. A.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Tumlinson, James H.] Penn State Univ, Dept Entomol, Ctr Chem Ecol, University Pk, PA 16802 USA. [Borgemeister, Christian] Univ Bonn, Ctr Dev Res, D-53113 Bonn, Germany. RP Torto, B (reprint author), Int Ctr Insect Physiol & Ecol, Box 30772, Nairobi 00100, Kenya. EM btorto@icipe.org FU Centre for Medical, Agricultural, and Veterinary Entomology, US Department of Agriculture; US National Institutes of Health, National Institute of Allergy and Infectious Diseases [R01A1077722]; World Federation of Scientists FX We are grateful to all of the staff at the icipe Mbita and Duduville campuses, who provided support without which our research would not have been possible. Special thanks are extended to Woodbridge A. Foster for critical review of the manuscript and Daisy Salifu for assistance with the statistical analysis. We also appreciate the technical assistance from George Omweri of icipe's St Jude clinic in Mbita; Tom Guda and his team of vector competence unit and the staff at the insectary unit at Mbita; and Onesmus Wanyama, Milka Gitau, and Richard Ochieng' at icipe Duduville. We thank Simon Mathenge (formerly of the Botany Department, University of Nairobi) for help in identification of plants. This study was funded in part by Centre for Medical, Agricultural, and Veterinary Entomology, US Department of Agriculture and by the US National Institutes of Health, National Institute of Allergy and Infectious Diseases grant R01A1077722 to Woodbridge A. Foster. V.O.N. was supported by icipe and the World Federation of Scientists. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 31 TC 19 Z9 19 U1 6 U2 44 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0960-9822 EI 1879-0445 J9 CURR BIOL JI Curr. Biol. PD JAN 20 PY 2014 VL 24 IS 2 BP 217 EP 221 DI 10.1016/j.cub.2013.12.022 PG 5 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 293AS UT WOS:000329944400031 PM 24412210 ER PT J AU Suryawan, A Davis, TA AF Suryawan, Agus Davis, Teresa A. TI Regulation of protein degradation pathways by amino acids and insulin in skeletal muscle of neonatal pigs SO JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY LA English DT Article DE Amino acids; Autophagy; Insulin; Leucine; Muscle; Neonate; Protein degradation; Protein synthesis; Swine; Ubiquitin ID TRANSLATION INITIATION; MAMMALIAN DEVELOPMENT; MOLECULAR-MECHANISMS; UBIQUITIN LIGASES; AUTOPHAGY; PHOSPHORYLATION; ACTIVATION; ATROPHY; STIMULATION; ATROGIN-1 AB Background: The rapid gain in lean mass in neonates requires greater rates of protein synthesis than degradation. We previously delineated the molecular mechanisms by which insulin and amino acids, especially leucine, modulate skeletal muscle protein synthesis and how this changes with development. In the current study, we identified mechanisms involved in protein degradation regulation. In experiment 1, 6- and 26-d-old pigs were studied during 1) euinsulinemic-euglycemic-euaminoacidemic, 2) euinsulinemic-euglycemic-hyperaminoacidemic, and 3) hyperinsulinemic-euglycemic-euaminoacidemic clamps for 2 h. In experiment 2, 5-d-old pigs were studied during 1) euinsulinemic-euglycemic-euaminoacidemic-euleucinemic, 2) euinsulinemic-euglycemic-hypoaminoacidemic-hyperleucinemic, and 3) euinsulinemic-euglycemic-euaminoacidemic-hyperleucinemic clamps for 24 h. We determined in muscle indices of ubiquitin-proteasome, i.e., atrogin-1 (MAFbx) and muscle RING-finger protein-1 (MuRF1) and autophagy-lysosome systems, i.e., unc51-like kinase 1 (UKL1), microtubule-associated protein light chain 3 (LC3), and lysosomal-associated membrane protein 2 (Lamp-2). For comparison, we measured ribosomal protein S6 (rpS6) and eukaryotic initiation factor 4E (eIF4E) activation, components of translation initiation. Results: Abundance of atrogin-1, but not MuRF1, was greater in 26-than 6-d-old pigs and was not affected by insulin, amino acids, or leucine. Abundance of ULK1 and LC3 was higher in younger pigs and not affected by treatment. The LC3-II/LC3-I ratio was reduced and ULK1 phosphorylation increased by insulin, amino acids, and leucine. These responses were more profound in younger pigs. Abundance of Lamp-2 was not affected by treatment or development. Abundance of eIF4E, but not rpS6, was higher in 6-than 26-d-old-pigs but unaffected by treatment. Phosphorylation of eIF4E was not affected by treatment, however, insulin, amino acids, and leucine stimulated rpS6 phosphorylation, and the responses decreased with development. Conclusions: The rapid growth of neonatal muscle is in part due to the positive balance between the activation of protein synthesis and degradation signaling. Insulin, amino acids, and, particularly, leucine, act as signals to modulate muscle protein synthesis and degradation in neonates. C1 [Suryawan, Agus; Davis, Teresa A.] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. RP Davis, TA (reprint author), Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. EM tdavis@bcm.edu FU National Institute of Arthritis and Musculoskeletal and Skin Diseases [AR-044474]; National Institute of Child Health and Human Development [HD-072891]; USDA/ARS [6250-510000-055] FX This work is a publication of the US Department of Agriculture/Agricultural Research Service (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine. This project has been funded in part by National Institute of Arthritis and Musculoskeletal and Skin Diseases Grant AR-044474 (T. A. Davis), National Institute of Child Health and Human Development HD-072891 (T. A. Davis), and by the USDA/ARS under Cooperative Agreement no. 6250-510000-055 (T. A. Davis). The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 65 TC 11 Z9 11 U1 3 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2049-1891 J9 J ANIM SCI BIOTECHNO JI J. Anim. Sci. Biotechnol. PD JAN 17 PY 2014 VL 5 AR 8 DI 10.1186/2049-1891-5-8 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA AD4AZ UT WOS:000333189600001 PM 24438646 ER PT J AU Werner, SJ Buchholz, R Tupper, SK Pettit, SE Ellis, JW AF Werner, Scott J. Buchholz, Richard Tupper, Shelagh K. Pettit, Susan E. Ellis, Jeremy W. TI Functional significance of ultraviolet feeding cues in wild turkeys SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE Conditioned avoidance; Eimeria spp.; Foraging behavior; Meleagris gallopavo; Postingestive consequence; Visual cue ID MELEAGRIS-GALLOPAVO; EIMERIA-MELEAGRIMITIS; SPECTRAL SENSITIVITY; FLIGHT CONTROL(TM); PLANTED RICE; BIRDS; ANTHRAQUINONE; COLOR; FRUIT; VISION AB Most birds are able to sense ultraviolet (UV) visual signals. Ultraviolet wavelengths are used for plumage signaling and sexual selection among birds. The aim of our study was to determine if UV cues are also used for the process of food selection in wild turkeys (Meleagris gallopavo). We used avoidance. conditioning to test the hypothesis that UV feeding cues can be used functionally for foraging behavior in wild turkeys. Female turkeys exhibited no avoidance of untreated food and 75-98% avoidance of food treated with an UV-absorbent, postingestive repellent (0.5-4% anthraquinone; wt./wt) during repellent exposure. Male turkeys exhibited 78-99% avoidance of food treated with 0.5-4% anthraquinone. Female and male turkeys that consumed more than 200 mg and 100 mg of anthraquinone, respectively, subsequently avoided food treated only with an UV-absorbent cue. In contrast, unconditioned females consumed 58% more food treated with the UV-absorbent cue than untreated food. Thus, wild turkeys do not prefer foods associated with UV wavelengths regardless of feeding experience. We also observed 1) a weak negative correlation between body condition and intestinal parasite infection and 2) moderate, positive correlations between consumption of food treated with the conditioned UV cue and intestinal parasite infection among male turkeys. The UV feeding cue was used to maintain food avoidance during the four days subsequent to postingestive conditioning. Moreover, the consequences of consuming food treated with the postingestive, UV-absorbent repellent were necessary for conditioned avoidance of the UV-absorbent cue. These findings suggest functional significance of UV feeding cues for avian foraging behavior, the implications of which will enable subsequent investigations regarding the sensory physiology and behavioral ecology of wild birds. Published by Elsevier Inc. C1 [Werner, Scott J.; Tupper, Shelagh K.; Pettit, Susan E.; Ellis, Jeremy W.] USDA, APHIS, WS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Buchholz, Richard] Univ Mississippi, Dept Biol, University, MS 38677 USA. RP Werner, SJ (reprint author), USDA, APHIS, WS, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Scott.J.Werner@aphis.usda.gov OI Ellis, Jeremy/0000-0003-4512-0906 FU California Department of Food and Agriculture's Specialty Crop Block Grant [SCB10034] FX We thank M. L Avery, B. F. Blackwell, B. A. Kimball, G. M. Linz, F. D. Provenza, and M. E. Tobin for their thoughtful review of our manuscript P. B. Fioranelli (Mississippi Field Station - National Wildlife Research Center, Starkville, MS, U.S.A.) provided dedicated assistance with constructing and disassembling enclosures for our hen study. We also thank the analytical chemistry unit at the National Wildlife Research Center (Fort Collins, CO, U.S.A.) for performing anthraquinone residue analyses. Arkion Life Sciences (New Castle, DE, U.S.A.) provided the Avipel (R) repellent and Acros Organics (Fair Lawn, NJ, U.S.A.) provided the Aeroxide (R) P25 for our study. Corporate collaborations do not imply endorsement by the United States Department of Agriculture. This project was supported in part by California Department of Food and Agriculture's Specialty Crop Block Grant to S.J.W. (Grant Agreement SCB10034). NR 47 TC 3 Z9 3 U1 3 U2 24 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD JAN 17 PY 2014 VL 123 BP 162 EP 167 DI 10.1016/j.physbeh.2013.10.010 PG 6 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA 293FJ UT WOS:000329956500023 PM 24432358 ER PT J AU Dubey, JP Ness, SL Kwok, OCH Choudhary, S Mittel, LD Divers, TJ AF Dubey, J. P. Ness, S. L. Kwok, O. C. H. Choudhary, S. Mittel, L. D. Divers, T. J. TI Seropositivity of Toxoplasma gondii in domestic donkeys (Equus asinus) and isolation of T. gondii from farm cats SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Donkeys (Equus asinus); Serology; Cats; Isolation; Genotyping ID CONGENITAL TOXOPLASMOSIS; CLINICAL FINDINGS; HIGH PREVALENCE; SEA OTTERS; GENOTYPE; EQUIDS; BRAZIL; SEROPREVALENCE; ANTIBODIES; CALIFORNIA AB Donkeys (Equus asinus) are used as both companion and working animals throughout the world and in some countries, their meat and milk are used for human consumption. Here we report the first serological survey of Toxoplasma gondii in donkeys in the United States. Serum samples from 373 donkeys from eight farms in five states were tested for T. gondii antibodies by the modified agglutination test (MAT). Twenty-four of 373 (6.4%) of donkeys were seropositive, with MAT titers ranging from 25 to >= 200. All seropositive donkeys were Miniature breed. Seropositivity prevalence was 7.0% in female donkeys (20/282) and 4.1% in male donkeys (4/91). No donkeys less than 24 months of age (129) were seropositive, suggesting postnatal transmission of infection. Domestic cats were present on six of the eight farms. Three cats from one farm had MAT titers of 200. Viable T. gondii was isolated from the hearts of two cats, but not from brain tissues. Genotyping of isolate DNA extracted from culture-derived tachyzoites using 10 PCR-restriction fragment length polymorphism (RFLP) markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, 1358 and Apico lad) revealed that both isolates were clonal Type II (ToxoDB PCR-RFLP genotype #1). This is the first serological survey for T. gondii in donkeys in the United States, and suggests that donkey milk and meat should be considered as a potential source for human infection. The role of barn cats in the transmission of T. gondii to donkeys on farms warrents further investigation. (C) 2013 Elsevier B.V. All rights reserved. C1 [Dubey, J. P.; Kwok, O. C. H.; Choudhary, S.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Ness, S. L.; Divers, T. J.] Cornell Univ, Coll Vet Med, Dept Clin Sci, Ithaca, NY 14853 USA. [Mittel, L. D.] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.dubey@ars.usda.gov NR 40 TC 7 Z9 8 U1 0 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JAN 17 PY 2014 VL 199 IS 1-2 BP 18 EP 23 DI 10.1016/j.vetpar.2013.09.027 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 294ZX UT WOS:000330087500003 PM 24140163 ER PT J AU Del Coco, VF Cordoba, MA Bilbao, G Castro, PD Basualdo, JA Santin, M AF Del Coco, Valeria F. Alejandra Cordoba, Maria Bilbao, Gladys de Almeida Castro, Pinto Angel Basualdo, Juan Santin, Monica TI First report of Enterocytozoon bieneusi from dairy cattle in Argentina SO VETERINARY PARASITOLOGY LA English DT Article DE Argentina; Cattle; Enterocytozoon bieneusi; Genotype; Microsporidia; PCR ID UNITED-STATES; GENOTYPE; MICROSPORIDIOSIS; PREVALENCE; IDENTIFICATION; CALVES; SWINE; PIGS AB Fecal specimens were obtained from a total of 70 dairy calves less than two months old on 11 municipalities in Buenos Aires, Argentina. After removal of fecal debris by sieving and sucrose flotation, specimens were subjected to PCR to detect the presence of Enterocytozoon bieneusi. PCR revealed a 14.3% of prevalence for E. bieneusi with 10 positive calves from 7 municipalities. Gene sequence analysis conducted in all samples positives by PCR revealed the presence of six genotypes; four previously reported in cattle as well as humans (D, I, J, and BEB4), one never reported in cattle before but previously reported in humans (EbpC), and one novel genotype (BEB10). These results constitute the first molecular characterization of E. bieneusi in Argentina, and suggest a potential risk of zoonotic transmission in this area. (C) 2013 Elsevier B.V. All rights reserved. C1 [Del Coco, Valeria F.; Alejandra Cordoba, Maria; Angel Basualdo, Juan] Univ Nacl La Plata, Catedra Microbiol & Parasitol, Fac Ciencias Med, RA-1900 La Plata, Buenos Aires, Argentina. [Del Coco, Valeria F.] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Alejandra Cordoba, Maria] Comis Invest Cient Prov Buenos Aires, La Plata, Buenos Aires, Argentina. [Bilbao, Gladys; de Almeida Castro, Pinto] Univ Nacl Ctr Prov Buenos Aires, Fac Ciencias Vet, Buenos Aires, DF, Argentina. [Santin, Monica] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. RP Del Coco, VF (reprint author), Univ Nacl La Plata, Catedra Microbiol & Parasitol, Fac Ciencias Med, RA-1900 La Plata, Buenos Aires, Argentina. EM vdelcoco@aetos.med.unlp.edu.ar OI Basualdo, Juan/0000-0001-9126-3719 NR 19 TC 19 Z9 19 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JAN 17 PY 2014 VL 199 IS 1-2 BP 112 EP 115 DI 10.1016/j.vetpar.2013.09.024 PG 4 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 294ZX UT WOS:000330087500014 PM 24126086 ER PT J AU Smith-Hammond, CL Swatek, KN Johnston, ML Thelen, JJ Miernyk, JA AF Smith-Hammond, Colin L. Swatek, Kirby N. Johnston, Mark L. Thelen, Jay J. Miernyk, Jan A. TI Initial description of the developing soybean seed protein Lys-N-epsilon -acetylome SO JOURNAL OF PROTEOMICS LA English DT Article DE Glycine max; Lysine acetylation; Collision-induced dissociation (CID); Electron transfer dissociation (ETD); Data-dependent decision tree ID TANDEM MASS-SPECTRA; SUBCELLULAR-LOCALIZATION; INTRINSIC DISORDER; CELLULAR FUNCTIONS; TOXOPLASMA-GONDII; SEARCH ALGORITHM; DIVERSE FUNCTION; DATABASE SEARCH; IMAGE-ANALYSIS; WEB SERVER AB Characterization of the myriad protein posttranslational modifications (PTM) is a key aspect of proteome profiling. While there have been previous studies of the developing soybean seed phospho-proteome, herein we present the first analysis of non-histone acetylation in this system. In recent years there have been reports that lysine acetylation is widespread, affecting thousands of proteins in diverse species from bacteria to mammals. Recently preliminary descriptions of the protein lysine acetylome from the plants Arabidopsis thaliana and Vitis vinifera have been reported. Using a combination of immunoenrichment and mass spectrometry-based techniques, we have identified over 400 sites of lysine acetylation in 245 proteins from developing soybean (Glycine max (L.) Men., cv. Jack) seeds, which substantially increases the number of known plant N-epsilon-lysineacetylation sites. Results of functional annotation indicate acetyl-proteins are involved with a host of cellular activities. In addition to histones, and other proteins involved in RNA synthesis and processing, acetyl-proteins participate in signaling, protein folding, and a plethora of metabolic processes. Results from in silico localization indicate that lysine-acetylated proteins are present in all major subcellular compartments. In toto, our results establish developing soybean seeds as a physiologically distinct addendum to Arabidopsis thaliana seedlings for functional analysis of protein Lys-N-epsilon-acetylation. Biological significance Several modes of peptide fragmentation and database search algorithms are incorporated to identify, for the first time, sites of lysine acetylation on a plethora of proteins from developing Soybean seeds. The contributions of distinct techniques to achieve increased coverage of the lysine acetylome are compared, providing insight to their respective benefits. Acetyl-proteins and specific acetylation sites are characterized, revealing intriguing similarities as well as differences with those previously identified in other plant and non-plant species. Published by Elsevier B.V. C1 [Smith-Hammond, Colin L.; Swatek, Kirby N.; Thelen, Jay J.; Miernyk, Jan A.] Univ Missouri, Div Biochem, Columbia, MO USA. [Smith-Hammond, Colin L.; Swatek, Kirby N.; Thelen, Jay J.; Miernyk, Jan A.] Univ Missouri, Interdisciplinary Plant Grp, Columbia, MO USA. [Johnston, Mark L.; Miernyk, Jan A.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO USA. RP Miernyk, JA (reprint author), Univ Missouri, USDA ARS, PGRU, 102 Curtis Hall, Columbia, MO 65211 USA. EM colin.smithhammond@mail.missouri.edu; knsty4@mail.missouri.edu; mark.johnston@ars.usda.gov; thelenj@missouri.edu; jan.miemyk@ars.usda.gov FU NSF; ILSI-Health Environmental Science Institute; USDA, Agricultural Research Service, NSF; Nichols Foundation FX Research in JJT's lab is supported by the NSF and ILSI-Health Environmental Science Institute, and in JAM's lab by the USDA, Agricultural Research Service, NSF, and the Nichols Foundation. The authors are unaware of any conflicts of interest, real or perceived, that might bias this study. Funding sources had no direct involvement in the design, execution, or publication of this study. Data mining with MS-GFDB was performed with the high performance computing resources at the University of Missouri Bioinforrnatics Consortium and was facilitated by Perl scripts developed by Dr. Christopher Bottoms of the Informatics Research Core Facility (ircf.met.missouri.edu). We additionally acknowledge the use of software, such as Peptide Fragmentation Modeller and Protein Coverage Summarizer, from PNNL (omics.pnl.gov), and are grateful to the PRIDE Team for their efforts. NR 84 TC 14 Z9 14 U1 1 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 EI 1876-7737 J9 J PROTEOMICS JI J. Proteomics PD JAN 16 PY 2014 VL 96 BP 56 EP 66 DI 10.1016/j.jprot.2013.10.038 PG 11 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA AB6RD UT WOS:000331916300006 PM 24211405 ER PT J AU Kohnke, T Elder, T Theliander, H Ragauskas, AJ AF Koehnke, Tobias Elder, Thomas Theliander, Hans Ragauskas, Arthur J. TI Ice templated and cross-linked xylan/nanocrystalline cellulose hydrogels SO CARBOHYDRATE POLYMERS LA English DT Article DE Cellulose nanocrystals; Cellulose whiskers; Freeze-casting; Hydrogel; Xylan ID NUCLEAR-MAGNETIC-RESONANCE; ADSORPTION CHARACTERISTICS; PERIODATE-OXIDATION; PHYSICAL-PROPERTIES; XYLAN DERIVATIVES; HEMICELLULOSES; WOOD; POLYSACCHARIDES; CRYSTALLINITY; BIOMATERIALS AB Structured xylan-based hydrogels, reinforced with cellulose nanocrystals (CNCs), have successfully been prepared from water suspensions by cross-linking during freeze-casting. In order to induce cross-linking during the solidification/sublimation operation, xylan was first oxidized using sodium periodate to introduce dialdehydes. The oxidized xylan was then mixed with CNCs after which the suspension was frozen unidirectionally in order to control the ice crystal formation and by that the pore morphology of the material. Finally the ice crystal templates were removed by freeze-drying. During the freeze-casting process hemiacetal bonds are formed between the aldehyde groups and hydroxyl groups, either on other xylan molecules or on CNCs, which cross-links the system. The proposed cross-linking reaction was confirmed by using cross-polarization magic angle spinning (CP/MAS) nuclear magnetic resonance (NMR) spectroscopy. The pore morphology of the obtained materials was analyzed by scanning electron microscopy (SEM). The materials were also tested for compressive strength properties, both in dry and water swollen state. All together this study describes a novel combined freeze-casting/cross-linking process which enables fabrication of nanoreinforced biopolymer-based hydrogels with controlled porosity and 3-D architecture. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Koehnke, Tobias; Ragauskas, Arthur J.] Georgia Inst Technol, Inst Paper Sci & Technol, Atlanta, GA 30332 USA. [Koehnke, Tobias; Theliander, Hans] Chalmers, Dept Chem & Biol Engn, SE-41296 Gothenburg, Sweden. [Elder, Thomas] US Forest Serv, USDA, So Res Stn, Pineville, LA 71360 USA. RP Ragauskas, AJ (reprint author), Georgia Inst Technol, Inst Paper Sci & Technol, Atlanta, GA 30332 USA. EM art.ragauskas@ipst.gatech.edu OI Kohnke, Tobias/0000-0003-1259-6414; Ragauskas, Arthur/0000-0002-3536-554X FU Gunnar Nicholson Foundation FX The authors gratefully acknowledge financial support from the Gunnar Nicholson Foundation. Authors would also like to thank Angela Lin for training and use of equipment in the Biomechanics Core Facility at the Parker H. Petit Institute for Bioengineering and Bioscience at Georgia Institute of Technology, USA. Marcus Foston at Georgia Institute of Technology, USA, is acknowledged for assistance during NMR spectroscopy analyses. NR 39 TC 11 Z9 12 U1 20 U2 160 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD JAN 16 PY 2014 VL 100 SI SI BP 24 EP 30 DI 10.1016/j.carbpol.2013.03.060 PG 7 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA 295BQ UT WOS:000330092000005 PM 24188834 ER PT J AU Robertson, GH Cao, TK Gregorski, KS Hurkman, WJ Tanaka, CK Chiou, BS Glenn, GM Orts, WJ AF Robertson, George H. Cao, Trung K. Gregorski, Kay S. Hurkman, William J. Tanaka, Charlene K. Chiou, Bor-Sen Glenn, Gregory M. Orts, William J. TI Modification of Vital Wheat Gluten with Phosphoric Acid to Produce High Free Swelling Capacity SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE biopolymers and renewable polymers; adsorption; crosslinking; gels; proteins ID 2-DIMENSIONAL GEL-ELECTROPHORESIS; PROTEINS; SOLUBILITY; SEPARATION; ETHANOL; FLOUR AB Wheat gluten reacts with phosphoric acid in the presence of urea to produce natural superabsorbent gels. Fourier Transform Infra-red (FT-IR) spectroscopy and two-dimensional gel electrophoresis (2DE) reveal chemical changes from the reaction. Temperatures above 120 degrees C and dry conditions create the opportunity for reaction. FT-IR analyses confirm the formation of esters, carbamates, and phosphoramides on the gluten samples. 2DE protein composition topographies indicate a shift in the isoelectric point (pI) to lower values along with extensive inter-protein linkages. A free swelling capacity (FSC) in excess of 85X the mass of the converted gluten is obtainable using a conservative vacuum-assisted method to recover and quantify the properties of the wet gel. Other methods produce FSC values nearly twice as high. FSC for acid-treated gluten is lower for solutions containing solutes than the FSC for deionized water. Native gluten produces FSC values that are about 2% of those for treated gluten, but these values are less sensitive to the presence of ionic solutes and increase slightly in the presence of aqueous ethanol up to a mole fraction of 0.25.. (C) 2013 Wiley Periodicals, Inc. C1 [Robertson, George H.; Cao, Trung K.; Gregorski, Kay S.; Hurkman, William J.; Tanaka, Charlene K.; Chiou, Bor-Sen; Glenn, Gregory M.; Orts, William J.] ARS, USDA, Pacific West Area, Western Reg Res Ctr, Albany, CA 94710 USA. RP Robertson, GH (reprint author), ARS, USDA, Pacific West Area, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM george.robertson@ars.usda.gov NR 21 TC 0 Z9 0 U1 4 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD JAN 15 PY 2014 VL 131 IS 2 AR 39440 DI 10.1002/app.39440 PG 11 WC Polymer Science SC Polymer Science GA AH6QP UT WOS:000336256000003 ER PT J AU Nemec, KT Allen, CR Danielson, SD Helzer, CJ AF Nemec, Kristine T. Allen, Craig R. Danielson, Stephen D. Helzer, Christopher J. TI Responses of predatory invertebrates to seeding density and plant species richness in experimental tallgrass prairie restorations SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Grassland restoration; Tallgrass prairie; Predatory invertebrates; Natural enemies; Plant richness; Ecosystem services ID MIDWESTERN UNITED-STATES; AGRICULTURAL LANDSCAPES; BIOLOGICAL-CONTROL; GRASSLAND RESTORATION; ARTHROPOD DIVERSITY; CARABID ASSEMBLAGES; BEETLES COLEOPTERA; NATURAL ENEMIES; GROUND BEETLES; PEST-CONTROL AB In recent decades, agricultural producers and non-governmental organizations have restored thousands of hectares of former cropland in the central United States with native grasses and forbs. However, the ability of these grassland restorations to attract predatory invertebrates has not been well documented, even though predators provide an important ecosystem service to agricultural producers by naturally regulating herbivores. This study assessed the effects of plant richness and seeding density on the richness and abundance of surface-dwelling (ants, ground beetles, and spiders) and aboveground (ladybird beetles) predatory invertebrates. In the spring of 2006, twenty-four 55 m x 55 m-plots were planted to six replicates in each of four treatments: high richness (97 species typically planted by The Nature Conservancy), at low and high seeding densities, and low richness (15 species representing a typical Natural Resources Conservation Service Conservation Reserve Program mix, CP25), at low and high seeding densities. Ants, ground beetles, and spiders were sampled using pitfall traps and ladybird beetles were sampled using sweep netting in 2007-2009. The abundance of ants, ground beetles, and spiders showed no response to seed mix richness or seeding density but there was a significant positive effect of richness on ladybird beetle abundance. Seeding density had a significant positive effect on ground beetle and spider species richness and Shannon-Weaver diversity. These results may be related to differences in the plant species composition and relative amount of grass basal cover among the treatments rather than richness. Published by Elsevier B.V. C1 [Nemec, Kristine T.] Univ Nebraska, Nebraska Cooperat Fish & Wildlife Res Unit, Lincoln, NE 68583 USA. [Allen, Craig R.] Univ Nebraska, Nebraska Cooperat Fish & Wildlife Res Unit, US Geol Survey, Lincoln, NE 68583 USA. [Danielson, Stephen D.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Helzer, Christopher J.] Nature Conservancy, Eastern Nebraska Project Off, Aurora, NE 68818 USA. RP Nemec, KT (reprint author), USDA ARS, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM kristinenemec14@gmail.com; callen3@unl.edu; sdanielson1@unl.edu; chelzer@tnc.org FU James S. McDonnell Foundation; Nebraska Game and Parks Commission; Center for Great Plains Studies grant; The Nature Conservancy; National Science Foundation Integrative Graduate Education and Research Traineeship at the University of Nebraska-Lincoln (NSF) [0903469]; U.S. Geological Survey; University of Nebraska-Lincoln; United States Fish and Wildlife Service; Wildlife Management Institute FX We thank Katy Dornbos, Michelle Hellman, and Elizabeth Manley for their assistance with fieldwork and processing of invertebrate specimens, including identification of ladybird beetles. John Nemec assisted with installing pitfall traps. We thank those who made taxonomic determinations of ants (James Trager, James Kalisch, Andre Francoeur, Elizabeth Manley), spiders (Hank Guarisco), and ground beetles (Foster Purrington and Peter Messer). We also thank The Nature Conservancy for site access and lodging. An earlier version of this manuscript was improved by comments from Steve Spomer and LaReesa Wolfenbarger. Financial support for this project was provided by the James S. McDonnell Foundation, a State Wildlife Grant from the Nebraska Game and Parks Commission, a Center for Great Plains Studies grant, and a J.E. Weaver Grant from The Nature Conservancy. Kristine Nemec received support through the National Science Foundation Integrative Graduate Education and Research Traineeship at the University of Nebraska-Lincoln (NSF #0903469). The Nebraska Cooperative Fish and Wildlife Research Unit is jointly supported by a cooperative agreement between the U.S. Geological Survey, the Nebraska Game and Parks Commission, the University of Nebraska-Lincoln, the United States Fish and Wildlife Service, and the Wildlife Management Institute. Reference to trade names does not imply endorsement by the authors or the U.S. Government. NR 61 TC 5 Z9 6 U1 4 U2 43 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD JAN 15 PY 2014 VL 183 BP 11 EP 20 DI 10.1016/j.agee.2013.10.024 PG 10 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA AE5BJ UT WOS:000334002500002 ER PT J AU Reeves, JL Derner, JD Sanderson, MA Hendrickson, JR Kronberg, SL Petersen, MK Vermeire, LT AF Reeves, Justin L. Derner, Justin D. Sanderson, Matt A. Hendrickson, John R. Kronberg, Scott L. Petersen, Mark K. Vermeire, Lance T. TI Seasonal weather influences on yearling beef steer production in C-3-dominated Northern Great Plains rangeland SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Climate variability; Decision support tools; Grazing management; Model averaging; Northern mixed-grass prairie; Semiarid rangeland; Long-Term Agro-ecosystem Research (LTAR) ID MIXED-GRASS PRAIRIE; FORAGE PRODUCTION; GRAZING INTENSITY; STOCKING RATE; LEAFY SPURGE; TEMPERATURE; CATTLE; PRECIPITATION; RESPONSES; STRATEGIES AB In the face of an increasingly variable climate, long-term cattle weight gain datasets are rare, yet invaluable, for determining site-specific influences of seasonal weather patterns on cattle production. Here, we present a long-term (1936-2005) yearling Hereford steer dataset collected at the Northern Great Plains Research Laboratory (NPGRL) near Mandan, ND, USA. Data were analyzed using weighted AICc model averaging to examine the effects of spring (April-June) and summer (July-September) temperature and precipitation, as well as prior growing season (prior April-September) and prior fall/winter (prior October-March) precipitation on cattle production (kg/ha) under light (37.4 +/- 5.3 SD Animal Unit Days [AUD]/ha across all study years) and heavy (91.6 +/- 22.2 SD AUD/ha) stocking rates. Because Kentucky bluegrass (Poa pratensis L.) invaded the grassland at NPGRL in the early 1980s, we modeled cattle production separately for pre- (1936-1983) and post-invasion (1986-2005) years to determine if the plant community shift influenced sensitivity to seasonal weather patterns. Cattle production under heavy stocking was more sensitive to seasonal weather variability than under light stocking during both pre- and post-invasion years. Interestingly, the magnitude and robustness of coefficients changed between the pre- and post-invasion years, with seasonal weather patterns explaining more cattle production variation during the post-invasion years. Though cattle sensitivity to seasonal weather patterns differed between light and heavy stocking for both pre- and post-invasion years, invasion status did change cattle response to weather. For example, cattle production in P. pratensis invaded pastures was more heavily influenced by cool, wet springs and wet prior grazing seasons than was production in un-invaded pastures. For cattle stocked heavily in native pastures, wet winters more strongly increased cattle production than in invaded pastures. Published by Elsevier B.V. C1 [Reeves, Justin L.; Derner, Justin D.] USDA ARS, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA. [Sanderson, Matt A.; Hendrickson, John R.; Kronberg, Scott L.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Petersen, Mark K.; Vermeire, Lance T.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Reeves, JL (reprint author), USDA ARS, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA. EM Justin.Reeves@ars.usda.gov NR 50 TC 4 Z9 4 U1 3 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD JAN 15 PY 2014 VL 183 BP 110 EP 117 DI 10.1016/j.agee.2013.10.030 PG 8 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA AE5BJ UT WOS:000334002500013 ER PT J AU O'Connor, TM Cerin, E Hughes, SO Robles, J Thompson, DI Mendoza, JA Baranowski, T Lee, RE AF O'Connor, Teresia M. Cerin, Ester Hughes, Sheryl O. Robles, Jessica Thompson, Deborah I. Mendoza, Jason A. Baranowski, Tom Lee, Rebecca E. TI Psychometrics of the preschooler physical activity parenting practices instrument among a Latino sample SO INTERNATIONAL JOURNAL OF BEHAVIORAL NUTRITION AND PHYSICAL ACTIVITY LA English DT Article DE Physical activity; Parenting practices; Latino; Hispanic; Preschool child; Confirmatory factor analysis ID BODY-MASS INDEX; NOMINAL GROUP TECHNIQUE; HEALTH-PROMOTION; YOUNG-CHILDREN; ADOLESCENTS; VALIDATION; CONTEXT; ASSOCIATION; DIMENSIONS; CHALLENGES AB Background: Latino preschoolers (3-5 year old children) have among the highest rates of obesity. Low levels of physical activity (PA) are a risk factor for obesity. Characterizing what Latino parents do to encourage or discourage their preschooler to be physically active can help inform interventions to increase their PA. The objective was therefore to develop and assess the psychometrics of a new instrument: the Preschooler Physical Activity Parenting Practices (PPAPP) among a Latino sample, to assess parenting practices used to encourage or discourage PA among preschool-aged children. Methods: Cross-sectional study of 240 Latino parents who reported the frequency of using PA parenting practices. 95% of respondents were mothers; 42% had more than a high school education. Child mean age was 4.5 (+/- 0.9) years (52% male). Test-retest reliability was assessed in 20%, 2 weeks later. We assessed the fit of a priori models using Confirmatory factor analyses (CFA). In a separate sub-sample (35%), preschool-aged children wore accelerometers to assess associations with their PA and PPAPP subscales. Results: The a-priori models showed poor fit to the data. A modified factor structure for encouraging PPAPP had one multiple-item scale: engagement (15 items), and two single-items (have outdoor toys; not enroll in sport-reverse coded). The final factor structure for discouraging PPAPP had 4 subscales: promote inactive transport (3 items), promote screen time (3 items), psychological control (4 items) and restricting for safety (4 items). Test-retest reliability (ICC) for the two scales ranged from 0.56-0.85. Cronbach's alphas ranged from 0.5-0.9. Several sub-factors correlated in the expected direction with children's objectively measured PA. Conclusion: The final models for encouraging and discouraging PPAPP had moderate to good fit, with moderate to excellent test-retest reliabilities. The PPAPP should be further evaluated to better assess its associations with children's PA and offers a new tool for measuring PPAPP among Latino families with preschool-aged children. C1 [O'Connor, Teresia M.; Hughes, Sheryl O.; Robles, Jessica; Thompson, Deborah I.; Mendoza, Jason A.; Baranowski, Tom] Baylor Coll Med, ARS, USDA, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. [O'Connor, Teresia M.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Cerin, Ester] Univ Hong Kong, Inst Human Performance, Pokfulam, Hong Kong, Peoples R China. [Cerin, Ester] Deakin Univ, Sch Exercise & Nutr Sci, Ctr Phys Act & Nutr, Burwood, Australia. [Lee, Rebecca E.] Arizona State Univ, Coll Nursing & Hlth Innovat, Phoenix, AZ USA. [Mendoza, Jason A.] Univ Washington, Sch Med, Dept Pediat, Div Gen Pediat, Seattle, WA 98195 USA. RP O'Connor, TM (reprint author), Baylor Coll Med, ARS, USDA, Childrens Nutr Res Ctr,Dept Pediat, 1100 Bates St, Houston, TX 77030 USA. EM teresiao@bcm.edu RI Cerin, Ester/L-1271-2015; OI Cerin, Ester/0000-0002-7599-165X; Mendoza, Jason/0000-0003-0833-4358; Baranowski, Tom/0000-0002-0653-2222 FU NIH-Eunice Kennedy Shriver National Institute of Child Health & Human Development [R21HD060925]; US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, BCM through a cooperative agreement [USDA/ARS 6250-51000-053-20S]; [K07CA131178] FX This study was funded by NIH-Eunice Kennedy Shriver National Institute of Child Health & Human Development (R21HD060925). This work is a publication of the US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, BCM through a cooperative agreement (USDA/ARS 6250-51000-053-20S). JAM was supported, in part, by a career development award (K07CA131178). The contents of this publication do not necessarily reflect the views or policies of the USDA or NIH, nor does mention of organizations imply endorsement from the US government. The Spanish version is available upon request. NR 62 TC 5 Z9 5 U1 2 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1479-5868 J9 INT J BEHAV NUTR PHY JI Int. J. Behav. Nutr. Phys. Act. PD JAN 15 PY 2014 VL 11 AR 3 DI 10.1186/1479-5868-11-3 PG 14 WC Nutrition & Dietetics; Physiology SC Nutrition & Dietetics; Physiology GA AD0CD UT WOS:000332900600001 PM 24428935 ER PT J AU Jandl, R Rodeghiero, M Martinez, C Cotrufo, MF Bampa, F van Wesemael, B Harrison, RB Guerrini, IA Richter, DD Rustad, L Lorenz, K Chabbi, A Miglietta, F AF Jandl, Robert Rodeghiero, Mirco Martinez, Cristina Cotrufo, M. Francesca Bampa, Francesca van Wesemael, Bas Harrison, Robert B. Guerrini, Irae Amaral Richter, Daniel deB, Jr. Rustad, Lindsey Lorenz, Klaus Chabbi, Abad Miglietta, Franco TI Current status, uncertainty and future needs in soil organic carbon monitoring SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Soil monitoring; Soil organic carbon pool ID LONG-TERM EXPERIMENTS; LAND-USE CHANGE; GLOBAL CLIMATE-CHANGE; AGRICULTURAL SOILS; FOREST SOILS; STABILIZATION MECHANISMS; ACID FUMIGATION; MATTER DYNAMICS; REGIONAL-SCALE; POLICY SUPPORT AB Increasing human demands on soil-derived ecosystem services requires reliable data on global soil resources for sustainable development. The soil organic carbon (SOC) pool is a key indicator of soil quality as it affects essential biological, chemical and physical soil functions such as nutrient cycling, pesticide and water retention, and soil structure maintenance. However, information on the SOC pool, and its temporal and spatial dynamics is unbalanced. Even in well-studied regions with a pronounced interest in environmental issues information on soil carbon (C) is inconsistent Several activities for the compilation of global soil C data are under way. However, different approaches for soil sampling and chemical analyses make even regional comparisons highly uncertain. Often, the procedures used so far have not allowed the reliable estimation of the total SOC pool, partly because the available knowledge is focused on not clearly defined upper soil horizons and the contribution of subsoil to SOC stocks has been less considered. Even more difficult is quantifying SOC pool changes over time. SOC consists of variable amounts of labile and recalcitrant molecules of plant, and microbial and animal origin that are often operationally defined. A comprehensively active soil expert community needs to agree on protocols of soil surveying and lab procedures towards reliable SOC pool estimates. Already established long-term ecological research sites, where SOC changes are quantified and the underlying mechanisms are investigated, are potentially the backbones for regional, national, and international SOC monitoring programs. (C) 2013 Elsevier B.V. All rights reserved. C1 [Jandl, Robert] Forest Res Ctr BFW, A-1131 Vienna, Austria. [Rodeghiero, Mirco] Fdn Edmund Mach, IASMA Res & Innovat Ctr, Sustainable Agroecosyst & Bioresources Dept, I-38010 San Michele All Adige, TN, Italy. [Martinez, Cristina; Miglietta, Franco] CNR, Inst Biometeorol IBIMET, I-50145 Florence, Italy. [Martinez, Cristina; Miglietta, Franco] Fdn Edmund Mach, IASMA Res & Innovat Ctr, FoxLab, I-38010 San Michele All Adige, TN, Italy. [Cotrufo, M. Francesca] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Bampa, Francesca] Commiss European, DG Joint Res Ctr, Land Resource Management Unit H05, I-21027 Ispra, VA, Italy. [van Wesemael, Bas] Catholic Univ Louvain, Earth & Life Inst, Georges Lemaitre Ctr Earth & Climate Res, B-1348 Louvain, Belgium. [Harrison, Robert B.] Univ Washington, Coll Forest Resources, Seattle, WA 98195 USA. [Guerrini, Irae Amaral] Univ Estadual Paulista, UNESP, Fac Ciencias Agron, Dept Solo & Recursos Ambientais, Botucatu, SP, Brazil. [Richter, Daniel deB, Jr.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Rustad, Lindsey] USDA FS, Ctr Res Ecosyst Change, No Res Stn, Durham, NH 03824 USA. [Lorenz, Klaus] Inst Adv Sustainabil Studies, D-14467 Potsdam, Germany. [Chabbi, Abad] INRA, INRA UEFE, F-86600 Lusignan, France. RP Jandl, R (reprint author), Forest Res Ctr BFW, Seckendotff Gudent Weg 8, A-1131 Vienna, Austria. EM robert.jandl@bfw.gv.at; mirco.rodeghiero@fmach.it; cristina.martinez@fmach.it; francesca.cotrufo@colostate.edu; francesca.bampa@jrc.ec.europa.eu; bas.vanwesemael@uclouvain.be; robh@uow.edu; iguerrini@fca.unesp.br; drichter@duke.edu; rustad@maine.edu; klaus.lorenz@iass-potsdam.de; abad.chabbi@lusignan.inra.fr; miglietta@ibimet.cnr.it RI Miglietta, Franco/A-1257-2009; Cotrufo, M. Francesca/C-1614-2013; Rodeghiero, Mirco/G-8559-2011; OI Miglietta, Franco/0000-0003-1474-8143; Cotrufo, M. Francesca/0000-0002-6191-8953; Rodeghiero, Mirco/0000-0003-3228-4557; Martinez, Cristina/0000-0003-1592-9149 NR 100 TC 32 Z9 32 U1 9 U2 106 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JAN 15 PY 2014 VL 468 BP 376 EP 383 DI 10.1016/j.scitotenv.2013.08.026 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA AB4QW UT WOS:000331776000043 PM 24041605 ER PT J AU Potter, TL Hapeman, CJ McConnell, LL Harman-Fetcho, JA Schmidt, WF Rice, CP Schaffer, B AF Potter, Thomas L. Hapeman, Cathleen J. McConnell, Laura L. Harman-Fetcho, Jennifer A. Schmidt, Walter F. Rice, Clifford P. Schaffer, Bruce TI Endosulfan wet deposition in Southern Florida (USA) SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Insecticide; Rainfall; Water quality; Everglades; Biscayne; National Park ID PERSISTENT ORGANIC POLLUTANTS; CURRENT-USE PESTICIDES; ORGANOCHLORINE PESTICIDES; POLYCHLORINATED-BIPHENYLS; ATMOSPHERIC DEPOSITION; AGRICULTURAL ACTIVITY; SURFACE-WATER; GREAT-LAKES; AIR; BAY AB The atmosphere is an important transport route for semi-volatile pesticides like endosulfan. Deposition, which depends on physical-chemical properties, use patterns, and climatic conditions, can occur at local, regional, and global scales. Adverse human and ecological impact may result. We measured endosulfan wet deposition in precipitation over a 4-year period within an area of high agricultural use in Southern Florida (USA) and in nearby Biscayne and Everglades National Parks. Endosulfan's two isomers and degradate, endosulfan sulfate, were detected at high frequency with the order of detection and concentration being beta-endosulfan > et-en d sulfa n > endosulfan sulfate. Within the agricultural area, detection frequency (55 to 98%) mean concentrations (5 to 87 ng L-1) and total daily deposition (200 ng m(-2) day(-1)) exceeded values at other sites by 5 to 30-fold. Strong seasonal trends were also observed with values at all monitored sites significantly higher during peak endosulfan use periods when vegetable crops were produced. Relatively high deposition in the crop production area and observations that concentrations exceeded aquatic life toxicity thresholds at all sites indicated that endosulfan volatilization and wet deposition are of ecotoxicological concern to the region. This study emphasizes the need to include localized volatilization and deposition of endosulfan and other semi-volatile pesticides in risk assessments in Southern Florida and other areas with similar climatic and crop production profiles. Published by Elsevier B.V. C1 [Potter, Thomas L.] USDA ARS, Southeast Watershed Lab, Tifton, GA 31793 USA. [Hapeman, Cathleen J.; McConnell, Laura L.; Harman-Fetcho, Jennifer A.; Schmidt, Walter F.; Rice, Clifford P.] USDA ARS, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Schaffer, Bruce] Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA. RP Potter, TL (reprint author), USDA ARS, Southeast Watershed Lab, Tifton, GA 31793 USA. EM Tom.Potter@ars.usda.gov FU South Florida Water Management District [C12231]; USDA-ARS FX Numerous field and technical staff, students, and volunteers contributed to the success of the project. We especially thank Sally Belflower, Margie Whittle, S. Michael Gutierrez, Richard Curry, Kerry Sefton, Ramona Smith, Leticia Drakeford, and Peter Downey. Funding for this project was provided in part by the South Florida Water Management District contract number C12231 and the USDA-ARS intramural research under National Programs 211 Water Availability and Watershed Management and 212 Climate Change, Soils, and Emissions. NR 47 TC 5 Z9 5 U1 5 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JAN 15 PY 2014 VL 468 BP 505 EP 513 DI 10.1016/j.scitotenv.2013.08.070 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA AB4QW UT WOS:000331776000055 PM 24055666 ER PT J AU Chang, F Dutta, S Becnel, JJ Estep, AS Mascal, M AF Chang, Fei Dutta, Saikat Becnel, James J. Estep, Alden S. Mascal, Mark TI Synthesis of the Insecticide Prothrin and Its Analogues from Biomass-Derived 5-(Chloromethyl)furfural SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE biomass; furans; pyrethroids; synthesis; 5-(chloromethyl)furfural ID CONVERSION; CELLULOSE AB Prothrin, a synthetic pyrethroid insecticide, was synthesized from the biomass-derived platform chemical 5-(chloromethyl)furfural in six steps and overall 65% yield. Two structural analogues of prothrin were also prepared following the same synthetic approach. Preliminary testing of these furan-based pyrethroids against the yellow fever mosquito Aedes aegypti indicates promising insecticidal activities. C1 [Chang, Fei; Dutta, Saikat; Mascal, Mark] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA. [Becnel, James J.] ARS, CMAVE, USDA, Gainesville, FL 32608 USA. [Estep, Alden S.] Navy Entomol Ctr Excellence, Jacksonville, FL 32212 USA. RP Mascal, M (reprint author), Univ Calif Davis, Dept Chem, 1 Shields Ave, Davis, CA 95616 USA. EM mjmascal@ucdavis.edu NR 28 TC 10 Z9 10 U1 6 U2 26 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 15 PY 2014 VL 62 IS 2 BP 476 EP 480 DI 10.1021/jf4045843 PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 294BT UT WOS:000330018100018 PM 24354505 ER PT J AU Fang, WP Meinhardt, LW Mischke, S Bellato, CM Motilal, L Zhang, DP AF Fang, Wanping Meinhardt, Lyndel W. Mischke, Sue Bellato, Claudia M. Motilal, Lambert Zhang, Dapeng TI Accurate Determination of Genetic Identity for a Single Cacao Bean, Using Molecular Markers with a Nanofluidic System, Ensures Cocoa Authentication SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE authentication; chocolate; conservation; fair trade; fluidigm; food forensics; food adulteration; gourmet food; germplasm; molecular markers; Theobroma cacao; traceability; tropical tree ID THEOBROMA-CACAO; MULTILOCUS GENOTYPES; FOOD TRACEABILITY; DIVERSITY; IDENTIFICATION; POPULATIONS; VARIETIES; GENOMICS; PCR; L. AB Cacao (Theobroma cacao L.), the source of cocoa, is an economically important tropical crop. One problem with the premium cacao market is contamination with off-types adulterating raw premium material. Accurate determination of the genetic identity of single cacao beans is essential for ensuring cocoa authentication. Using nanofluidic single nucleotide polymorphism (SNP) genotyping with 48 SNP markers, we generated SNP fingerprints for small quantities of DNA extracted from the seed coat of single cacao beans. On the basis of the SNP profiles, we identified an assumed adulterant variety, which was unambiguously distinguished from the authentic beans by multilocus matching. Assignment tests based on both Bayesian clustering analysis and allele frequency clearly separated all 30 authentic samples from the non-authentic samples. Distance-based principle coordinate analysis further supported these results. The nanofluidic SNP protocol, together with forensic statistical tools, is sufficiently robust to establish authentication and to verify gourmet cacao varieties. This method shows significant potential for practical application. C1 [Fang, Wanping; Meinhardt, Lyndel W.; Mischke, Sue; Bellato, Claudia M.; Zhang, Dapeng] ARS, SPCL, BARC, USDA,BARC W, Beltsville, MD 20705 USA. [Fang, Wanping] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China. [Motilal, Lambert] Univ W Indies, Cocoa Res Ctr, St Augustine, Trinid & Tobago. RP Zhang, DP (reprint author), ARS, SPCL, BARC, USDA,BARC W, 10300 Baltimore Ave,Bldg 001,Room 223, Beltsville, MD 20705 USA. EM dapeng.zhang@ars.usda.gov FU ARS; USDA; Lindt and Sprungli chocolate company through the World Cocoa Foundation FX This work was funded by ARS, USDA, and by a financial gift from Lindt and Sprungli chocolate company through the World Cocoa Foundation. References to a company and/or product by the USDA are only for the purposes of information and do not imply approval or recommendation of the product to the exclusion of others that may also be suitable. NR 41 TC 7 Z9 7 U1 3 U2 34 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 15 PY 2014 VL 62 IS 2 BP 481 EP 487 DI 10.1021/jf404402v PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 294BT UT WOS:000330018100019 PM 24354624 ER PT J AU Lin, LG Chen, KY Khalek, WA Ward, JL Yang, H Chabi, B Wrutniak-Cabello, C Tong, Q AF Lin, Ligen Chen, Keyun Khalek, Waed Abdel Ward, Jack Lee, III Yang, Henry Chabi, Beatrice Wrutniak-Cabello, Chantal Tong, Qiang TI Regulation of Skeletal Muscle Oxidative Capacity and Muscle Mass by SIRT3 SO PLOS ONE LA English DT Article ID ACTIVATED PROTEIN-KINASE; FATTY-ACID OXIDATION; MYOSIN HEAVY-CHAIN; CALORIC RESTRICTION; TRANSGENIC MICE; SIRT3-MEDIATED DEACETYLATION; MITOCHONDRIAL-FUNCTION; LYSINE ACETYLATION; UBIQUITIN LIGASES; GENE-EXPRESSION AB We have previously reported that the expression of mitochondrial deacetylase SIRT3 is high in the slow oxidative muscle and that the expression of muscle SIRT3 level is increased by dietary restriction or exercise training. To explore the function of SIRT3 in skeletal muscle, we report here the establishment of a transgenic mouse model with muscle-specific expression of the murine SIRT3 short isoform (SIRT3M3). Calorimetry study revealed that the transgenic mice had increased energy expenditure and lower respiratory exchange rate (RER), indicating a shift towards lipid oxidation for fuel usage, compared to control mice. The transgenic mice exhibited better exercise performance on treadmills, running 45% further than control animals. Moreover, the transgenic mice displayed higher proportion of slow oxidative muscle fibers, with increased muscle AMPK activation and PPARd expression, both of which are known regulators promoting type I muscle fiber specification. Surprisingly, transgenic expression of SIRT3M3 reduced muscle mass up to 30%, likely through an up-regulation of FOXO1 transcription factor and its downstream atrophy gene MuRF-1. In summary, these results suggest that SIRT3 regulates the formation of oxidative muscle fiber, improves muscle metabolic function, and reduces muscle mass, changes that mimic the effects of caloric restriction. C1 [Lin, Ligen; Chen, Keyun; Ward, Jack Lee, III; Yang, Henry; Tong, Qiang] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Khalek, Waed Abdel; Chabi, Beatrice; Wrutniak-Cabello, Chantal] INRA, UMR Dynam Musculaire & Metab 866, F-34060 Montpellier, France. [Tong, Qiang] Baylor Coll Med, Dept Med, Houston, TX 77030 USA. [Tong, Qiang] Baylor Coll Med, Dept Mol Physiol & Biophys, Houston, TX 77030 USA. RP Tong, Q (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM qtong@bcm.tmc.edu FU American Heart Association [0665162Y]; U.S. Department of Agriculture [6250-51000-055]; Baylor Diabetes Endocrinology Research Center-Pilot & Feasibility Grant [P30 DK079638]; NIH [DK075978]; USDA ARS; PHS [DK 56338] FX This work was supported by the American Heart Association (0665162Y), a U.S. Department of Agriculture (6250-51000-055), Baylor Diabetes Endocrinology Research Center-Pilot & Feasibility Grant (P30 DK079638), and NIH (DK075978) to Q. T. Measurements of energy balance and food intake were performed in the Mouse Metabolic Research Unit at the USDA/ARS Children's Nutrition Research Center, which is supported by funds from the USDA ARS (www.bcm.edu/cnrc/mmru). The services provided by the Molecular Morphology Core Laboratory was sponsored by PHS grant DK 56338. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 71 TC 9 Z9 9 U1 2 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 15 PY 2014 VL 9 IS 1 AR e85636 DI 10.1371/journal.pone.0085636 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 297DH UT WOS:000330235100111 PM 24454908 ER PT J AU Garcia, RA Riner, SA Piazza, GJ AF Garcia, Rafael A. Riner, Stephanie A. Piazza, George J. TI Design of a Laboratory Method for Rapid Evaluation of Experimental Flocculants SO INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH LA English DT Article AB Reports of novel organic polymeric flocculants have become commonplace. The method used to test the effectiveness of these flocculants is most often the flocculation of a kaolin suspension in a jar test. The widely varying versions of this method that appear in the literature suffer from a range of weaknesses. The present research uses well-defined kaolin and confines testing to conditions in which the kaolin suspension is stable in the absence of a flocculant. The research examines all aspects of the conduct of the method, including clay dosing, mixing, settling time, and measurement to improve the sensitivity, reproducibility, and robustness of the method, and takes steps to avoid pitfalls that can reduce the validity of the method. Innovations include careful selection of the buffer system and instrument characteristics. Kaolin Clarification Effectiveness is introduced as a metric that gives a meaningful indication of the relative value of a novel flocculant while emphasizing the critical importance of test conditions. Together, the results form a set of recommended test conditions that should be useful for new flocculant research. C1 [Garcia, Rafael A.; Riner, Stephanie A.; Piazza, George J.] ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Garcia, RA (reprint author), ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM garcia@ars.usda.gov OI Garcia, Rafael A./0000-0002-5452-3929 NR 11 TC 3 Z9 3 U1 3 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0888-5885 J9 IND ENG CHEM RES JI Ind. Eng. Chem. Res. PD JAN 15 PY 2014 VL 53 IS 2 BP 880 EP 886 DI 10.1021/ie4036115 PG 7 WC Engineering, Chemical SC Engineering GA 294BX UT WOS:000330018500043 ER PT J AU Sadler, EJ Sudduth, KA Lerch, RN Baffaut, C Kitchen, NR AF Sadler, E. J. Sudduth, K. A. Lerch, R. N. Baffaut, C. Kitchen, N. R. TI A simple index explains annual atrazine transport from surface runoff-prone watersheds in the north-central USA SO HYDROLOGICAL PROCESSES LA English DT Article DE atrazine; vulnerability index; watershed; claypan; CEAP; Goodwater Creek Experimental Watershed ID LONG-TERM RESEARCH; HERBICIDE TRANSPORT; VULNERABILITY; SOIL AB Year-to-year dynamics in weather affect both the timing of application and the potential hydrologic transport of pesticides. Further, the most commonly used pesticides dissipate in the environment during the growing season. Interactions among these factors - hydrology, timing of application and dissipation kinetics - hinder the detection of temporal trends in transport. It is increasingly important to be able to discern such trends, to judge effectiveness of management practices or to determine whether observed changes were caused by management or weather. In previous work, a cumulative vulnerability index was developed to account for these three factors. It explained 63% of annual variation in atrazine load in the Goodwater Creek Experimental Watershed (GCEW). The objectives of the current work were (i) to generalize the cumulative vulnerability index to explicitly account for variation in watershed size, area treated with atrazine and average application rate; (ii) to test the overall performance on watersheds showing such variation; and (3) to test whether the generalized index properly accounted for the additional input parameters. The generalized index was tested using data from GCEW (73.7km(2)) and seven additional watersheds in the northeast Missouri claypan region that varied in size from 212 to 1180km(2) and from 4% to 23% of watershed area planted to corn or sorghum. Across 32 site-years, the generalized index explained 84% of variation in annual atrazine load. Further, tests of residuals showed no dependence on either watershed area or fraction of area planted to corn and sorghum, indicating that these parameters were properly integrated into the index. The performance of the index supports the conclusion that data obtained from GCEW is representative of the Mark Twain Lake Basin and likely the entire Central Claypan Major Land Resource Area. Published 2012. This article is a U.S. Government work and is in the public domain in the USA. C1 [Sadler, E. J.; Sudduth, K. A.; Lerch, R. N.; Baffaut, C.; Kitchen, N. R.] USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO USA. RP Sadler, EJ (reprint author), USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO USA. EM John.Sadler@ars.usda.gov FU USDA-NRCS under the CEAP project; USDA-ARS FX This research was conducted under USDA-ARS project "Missouri Conservation Effects Assessment Project', which was part of the ARS CEAP-WAS. Some funding was also provided by USDA-NRCS under the CEAP project. Cooperation by the Environmental Resource Coalition and the Missouri Corn Growers Association under Specific Cooperative Agreement 'Evaluating Effects of Best Management Practices for Crop Production in Mark Twain Lake Basin' facilitated sampling at the three northernmost sampling sites, particularly the efforts of Travis Howell of Environmental Resource Coalition. USDA-ARS support staff is also gratefully acknowledged, including Aaron Beshears, Bettina Coggeshall, Amber Spohn, Joe Absheer, Mark Olson, Bob Mahurin and Teri Oster. NR 18 TC 5 Z9 5 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD JAN 15 PY 2014 VL 28 IS 2 BP 210 EP 217 DI 10.1002/hyp.9544 PG 8 WC Water Resources SC Water Resources GA 285BO UT WOS:000329367900005 ER PT J AU Riche, M AF Riche, Marty TI Development of a semipurified test diet for determining amino acid requirements of Florida pompano Trachinotus carolinus reared under low-salinity conditions SO AQUACULTURE LA English DT Article DE Florida pompano; Trachinotus carolinus; Semipurified test diet; Crystalline amino acids; Whole body profile; Low-salinity conditions ID TROUT ONCORHYNCHUS-MYKISS; RECIRCULATING AQUACULTURE SYSTEMS; CHANOS-CHANOS FORSSKAL; SEABREAM SPARUS-AURATA; HYBRID STRIPED BASS; RAINBOW-TROUT; WHOLE-BODY; GROWTH-PERFORMANCE; OREOCHROMIS-NILOTICUS; NILE TILAPIA AB Two trials were conducted to develop a semipurified test diet for determining indispensable amino acid (IAA) requirements for Florida pompano Trachinotus carolinus. The objective of the first trial was to evaluate casein and corn gluten meal as principal intact protein sources, and the ability of Florida pompano to utilize L-crystalline amino acids in the diet. Eight isonitrogenous (42% crude protein) and isoenergetic (22.0 kJ/kg) diets were formulated. A 2 x 4 factorial was employed to evaluate the two intact proteins, each with four amino acid profiles that mimicked the ratio of IAA in pompano whole body, pompano unfertilized eggs, menhaden fish meal, or unsupplemented negative control. Growth and efficiency were significantly better (P < 0.05) for each of the IAA profiles with the casein-based diets. The objective of the second trial was to re-evaluate the same IAA profiles in 46% crude protein and 22.0 kJ/kg casein-based diets to evaluate their performance relative to each other and to a practical type fish meal-based control diet. Growth and feed conversion were significantly better (P < 0.05) in pompano fed the diet supplemented to mimic the ratio of the pompano whole body IAA profile than the other test diets, but not different from the control where they were 94% and 98% of the control diet, respectively. Significantly better growth and efficiency in fish fed the whole body IAA profile diet than the unsupplemented diet suggest that the crystalline amino acids were well utilized. However, a significantly lower (P < 0.05) protein productive value in fish fed the whole body IAA profile diet relative to the fish meal control suggests pompano may benefit from coating the amino acids prior to dietary inclusion, or more frequent feedings. A test diet incorporating casein at 200 g/kg dry diet and supplemented with crystalline amino acids to mimic the IAA ratio of the Florida pompano whole body is suitable for evaluating Florida pompano amino acid requirements. Published by Elsevier B. V. C1 [Riche, Marty] ARS, USDA, Ft Pierce, FL 34946 USA. RP Riche, M (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM marty.riche@ars.usda.gov FU USDA/Agricultural Research Project [6225-63000-007-00D] FX Special thanks to David Haley, Patrick Tracy, and Terhea Williams for their contributions and technical support. I thank Carl Webster for his critical review of this manuscript. This study was funded by the USDA/Agricultural Research Project No. 6225-63000-007-00D. All programs and services of the USDA are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status or handicap. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply endorsement by the USDA. Experimental protocols used in this study were conducted in accordance with USDA and national guidelines as set forth in the Animal Welfare Act. NR 71 TC 2 Z9 2 U1 1 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD JAN 15 PY 2014 VL 420 BP 49 EP 56 DI 10.1016/j.aquaculture.2013.10.038 PG 8 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 281AG UT WOS:000329071300007 ER PT J AU Bosworth, B Waldbieser, G AF Bosworth, Brian Waldbieser, Geoff TI General and specific combining ability of male blue catfish (Ictalurus furcatus) and female channel catfish (Ictalurus punctatus) for growth and carcass yield of their F-1 hybrid progeny SO AQUACULTURE LA English DT Article DE Hybrid; Catfish; Combining ability; Performance ID GENOTYPE-ENVIRONMENT INTERACTIONS; GENETIC-PARAMETERS; CROSSBRED PERFORMANCE; ECONOMIC TRAITS; RAINBOW-TROUT; BODY-WEIGHT; HERITABILITY; L.; SILURIFORMES; ASSIGNMENT AB U.S. aquaculture production of channel catfish female (Ictalurus punctatus) x blue catfish male (Ictalurus furcatus) F hybrids has increased substantially due to the hybrid's improved growth, survival, carcass yield and ease of harvest compared to the more commonly farmed channel catfish. However, information on the genetic architecture underlying phenotypic variation in the hybrid which is needed to develop an efficient genetic improvement program is lacking. Progeny from two separate factorial matings between male blue catfish and female channel catfish (ten males x seven females and twelve males x five females, respectively) were grown communally in earthen ponds and measured for carcass yield and weight at approximately 520 clays post-hatch. Parentage of progeny was assigned by inheritance of microsatellite genotypes. Progeny from 118 full-sib families, within 12 maternal half-sib and 22 paternal half-sib families, were measured for harvest weight (n = 1288) and carcass yield (n = 1101). Variance component estimates associated with clams (darn general combining ability and heritabilities based on darn half-sib families) were high, variance associated with sires (sire general combining ability and heritabilities based on sire half-sib families) were intermediate, and variance components associated with the darn x sire interactions (specific combining ability) were low for carcass yield and harvest weight. Data indicate that the genetic variance for carcass yield and harvest weight of F-1 hybrids was primarily additive. Selection for improved carcass yield and growth based on additive genetic merit of blue catfish male and channel catfish female parents should be effective for improving performance of hybrid progeny. Published by Elsevier B.V. C1 [Bosworth, Brian; Waldbieser, Geoff] USDA ARS, Catfish Genet Res Unit, Stoneville, MS 38776 USA. RP Bosworth, B (reprint author), USDA ARS, Catfish Genet Res Unit, POB 38, Stoneville, MS 38776 USA. EM brian.bosworth@ars.usda.gov NR 49 TC 5 Z9 6 U1 1 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD JAN 15 PY 2014 VL 420 BP 147 EP 153 DI 10.1016/j.aquaculture.2013.10.026 PG 7 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 281AG UT WOS:000329071300020 ER PT J AU MacDonald, RL Chen, HYH Palik, BJ Prepas, EE AF MacDonald, Rebecca L. Chen, Han Y. H. Palik, Brian J. Prepas, Ellie E. TI Influence of harvesting on understory vegetation along a boreal riparian-upland gradient SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Biodiversity; Boreal; Community assembly; Disturbance; Harvesting; Recovery ID FOREST MANAGEMENT; PLANT-COMMUNITIES; MICROCLIMATIC GRADIENTS; WESTERN WASHINGTON; SPECIES-DIVERSITY; SMALL STREAMS; OLD-GROWTH; RESILIENCE; DISTURBANCE; RESPONSES AB Management of riparian forests, and how they respond to disturbance, continues to be a focus of interest in the literature. Earlier studies on riparian plant community assembly following harvesting in the boreal forest have focused merely on highly contrasting microhabitats within a landscape, for example, stream-bank riparian habitat or upland habitat. Sustaining biodiversity and evaluating the success of riparian management requires an understanding of plant community assembly following overstory harvesting across the landscapes, e.g., along the entire riparian-upland gradient, and how they recover over time. Using pre- and post-harvest data, we quantified how riparian harvesting along a disturbance gradient affects understory plant species diversity, abundance, turnover, and composition. We also asked how these disturbance-response relationships vary from stream edge to uplands. We expect changes in the plant community will be greater and recovery to be slower with increased disturbance severity. Based on the ecology of riparian versus upland, we also expect harvesting to exert a stronger control with increasing distance from the stream channel through the colonization of early successional species and extirpation of extant species. We found that disturbance severity (i.e., from cut-to-shore) from harvesting exerted strong controls on the dynamics of understory vegetation in boreal riparian forests, which was still evident seven years after the disturbance event. However, the dynamic responses strongly differed with the distance from the stream channel. Specifically, streamside communities harvested with or without a 30 m riparian buffer, were maintained to a condition similar to uncut forests. However, upland communities were less resistant to overstory harvest and subsequently colonized by early successional species present in pre-harvest riparian plots. Furthermore, vascular and non-vascular plants exhibited contrasting responses in their richness, abundance, turnover, and composition. Our results indicate that streamside understory vegetation is inherently more resistant to stand-replacing disturbance than upland assemblages. (C) 2013 Elsevier B.V. All rights reserved. C1 [MacDonald, Rebecca L.; Chen, Han Y. H.; Prepas, Ellie E.] Lakehead Univ, Fac Nat Resources Management, Thunder Bay, ON P7B 5E1, Canada. [MacDonald, Rebecca L.] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada. [Palik, Brian J.] US Forest Serv, USDA, No Res Stn, Ctr Res Ecosyst Change, Grand Rapids, MN 55744 USA. RP MacDonald, RL (reprint author), Lakehead Univ, Fac Nat Resources Management, Thunder Bay, ON P7B 5E1, Canada. EM rmacdon2@lakeheadu.ca RI Chen, Han/A-1359-2008 OI Chen, Han/0000-0001-9477-5541 FU Natural Sciences and Engineering Research Council of Canada (NSERC) Industrial (Millar Western Forest Products Ltd.) Post-graduate Scholarship; NSERC FX We would like to thank S. Luke, T. McCready and M. Serediak for help in initiating this study; D. Kneeshaw, S. Biswas and two anonymous reviewers for providing valuable comments on an earlier version of the manuscript; and S. Eberwein, T. Butler, L. Salmon and M. LeRoux for their assistance in the field. Partial funding for R.L.M. was provided by a Natural Sciences and Engineering Research Council of Canada (NSERC) Industrial (Millar Western Forest Products Ltd.) Post-graduate Scholarship. This study is part of the NSERC funded CRD project FORWARD and partners (http://forward.lakeheadu.ca/). NR 60 TC 6 Z9 6 U1 2 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JAN 15 PY 2014 VL 312 BP 138 EP 147 DI 10.1016/j.foreco.2013.10.011 PG 10 WC Forestry SC Forestry GA 277EV UT WOS:000328802700015 ER PT J AU Keyser, TL Brown, PM AF Keyser, Tara L. Brown, Peter M. TI Long-term response of yellow-poplar to thinning in the southern Appalachian Mountains SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest thinning; Intermediate stand management; Basal area increment; Liriodendron tulipifera; Restoration ID ABIES L. KARST.; RADIAL GROWTH; STAND-DENSITY; SPATIAL HETEROGENEITY; FOREST MANAGEMENT; WESTERN OREGON; PONDEROSA PINE; STEM FORM; INCREMENT; YOUNG AB As the focus of forest management on many public lands shifts away from timber production and extraction to habitat, restoration, and diversity-related objectives, it is important to understand the long-term effects that previous management activities have on structure and composition to better inform current management decisions. In this paper, we analyzed 40 years of growth data to quantify (1) the long-term response of yellow-poplar to thinning across an age and site quality gradient, and (2) the longevity of any growth response yellow-poplar may have to thinning throughout the southern Appalachian Mountains. Between 1960 and 1963, 134-0.1 ha plots were established across an age and site quality gradient in yellow-poplar (Liriodendron tulipifera L) stands throughout the southern Appalachian Mountains. All plots were thinned from below, with post-thinning relative density categorized into three classes: low (relative density <0.25), moderate (relative density >= 0.25 but <0.35), and high (relative density >= 0.35 but <0.60). Using plot-level annual basal area increment (BAI; cm(2) yr(-1)) chronologies reconstructed from tree cores, average annual BAI was calculated for 10 years prior to thinning (BAI(pre)) and each following 10 year period thereafter (BAI(post)). Site index and age at the time of thinning had a positive effect on BAI(post). During the first 10 year period following thinning, annual BAI (at a site index = 32.3 m and age = 43) averaged (SE) 33.7 (1.6), 26.3 (1.3), and 21.6 (1.1) cm(2) yr(-1) in the low, moderate, and high density classes, respectively. Significant differences between low and moderate and low and high density classes remained throughout the duration of the study. During the 10 years post-thinning the ratio of BAI(post) to BAI(pre) (RBAI) was >1.0 in 92%, 86%, and 57% of plots in the low, moderate, and high density classes, respectively indicating an overall increase in growth relative to pre-thinning growth rates. By the fourth decade post-thinning the percentage of stands containing trees that possessed RBAI values >1.0 had fallen; however trees in 71% of the plots in the low density class continued to experience growth rates greater than those prior to thinning. We conclude the increase in growth is short-lived when density is reduced to moderate and high levels whereas the response of trees to more intense thinnings is long-lasting. Published by Elsevier B.V. C1 [Keyser, Tara L.] US Forest Serv, USDA, Southern Res Stn, Asheville, NC 28806 USA. [Brown, Peter M.] Rocky Mt Tree Ring Res Inc, Ft Collins, CO 80526 USA. RP Keyser, TL (reprint author), US Forest Serv, USDA, Southern Res Stn, 1577 Brevard Rd, Asheville, NC 28806 USA. EM tkeyser@fs.fed.us; pmb@rmtrr.org NR 54 TC 5 Z9 5 U1 4 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JAN 15 PY 2014 VL 312 BP 148 EP 153 DI 10.1016/j.foreco.2013.10.010 PG 6 WC Forestry SC Forestry GA 277EV UT WOS:000328802700016 ER PT J AU McPherson, BA Mori, SR Opiyo, SO Conrad, AO Wood, DL Bonello, P AF McPherson, Brice A. Mori, Sylvia R. Opiyo, Stephen O. Conrad, Anna O. Wood, David L. Bonello, Pierluigi TI Association between resistance to an introduced invasive pathogen and phenolic compounds that may serve as biomarkers in native oaks SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Coast live oak; Ellagic acid; Plant defense; Phytophthora ramorum; Quercus agrifolia; Sudden oak death ID PINE BLISTER RUST; PHYTOPHTHORA-RAMORUM; ANTIFUNGAL ACTIVITY; NORTH-AMERICA; CALIFORNIA; AMBROSIA; DISEASE; DEATH; BARK AB California coast live oaks (Quercus agrifolia Nee) have suffered large losses from sudden oak death, caused by the introduced oomycete Phytophthora ramorum. Infected mature coast live oaks exhibit cankers on the main stem that produce a viscous sap-derived exudate, referred to as bleeding. Subsequent attacks by ambrosia and bark beetles, followed by the activity of fungi introduced by these insects, have led to mortality levels greater than 50% since the mid-1990s. Despite an infection rate of 5% year(-1), asymptomatic trees still persist in many heavily infected stands after more than 15 years of exposure to the pathogen. We hypothesized that varying responses to P. ramorum, including apparent recovery from infections, reflected phenotypic differences in susceptibility. In this study we evaluated the relationship between the early development of symptoms in mature trees that were experimentally inoculated with P. ramorum and long-term survival. A logit model showed that external canker lengths measured 9 months following inoculation predicted survival 7 years later. We defined resistance to P. ramorum in the surviving trees as absence or cessation of bleeding after the 7 years of the study and absence of beetle attacks on bleeding trees. Probability of resistance was also predicted by external canker length measured 9 months after inoculation. Canker length distribution was consistent with quantitative resistance to P. ramorum. The role of plant chemistry in resistance was examined by quantifying soluble phenolics in phloem methanol extracts prepared from the surviving trees. A logistic regression model found that expression of resistance was associated with four phenolic compounds; ellagic acid and a partially characterized ellagic acid derivative, and two chromatographic peaks that represent two uncharacterized phenolic compounds. Ellagic acid and a crude methanol extract from coast live oak phloem (total phenolics) were fungistatic when assayed in vitro at physiologically relevant levels and total phenolics were fungicidal at the highest concentration tested. The association of certain phenolics with resistance may facilitate the use of biomarkers in minimally invasive assays to predict the response of trees to P. ramorum, thereby increasing the options for managing threatened forests. (C) 2013 Elsevier B.V. All rights reserved. C1 [McPherson, Brice A.; Wood, David L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Mori, Sylvia R.] US Forest Serv, USDA, Pacific Southwest Res Stn, Albany, CA 94710 USA. [Opiyo, Stephen O.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Mol & Cellular Imaging Ctr Columbus, Columbus, OH 43210 USA. [Conrad, Anna O.; Bonello, Pierluigi] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA. RP McPherson, BA (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM bmcpherson@berkeley.edu RI Bonello, Pierluigi/E-2776-2011; Opiyo, Stephen/A-9385-2013 FU University of California Exotic and Invasive Species Research Program (USDA-CSREES); USDA Forest Service Forest Health Protection; California Department of Forestry and Fire Protection; State University FX We thank Gabriela Ritokova and Madeline Hall for field assistance, Mann County Open Space District for access to study sites, and East Bay Regional Park District for access to coast live oaks in Briones Regional Park. Funding was provided by the University of California Exotic and Invasive Species Research Program (USDA-CSREES), USDA Forest Service Forest Health Protection, the California Department of Forestry and Fire Protection, and state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University. All assays were performed in a BSL-3 facility on the Ohio State University campus, Columbus, OH, under current USDA APHIS permits. Inoculum was kindly provided by Dr. David Rizzo, University of California, Davis. NR 34 TC 6 Z9 6 U1 2 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JAN 15 PY 2014 VL 312 BP 154 EP 160 DI 10.1016/j.foreco.2013.10.009 PG 7 WC Forestry SC Forestry GA 277EV UT WOS:000328802700017 ER PT J AU Brown, DJ Nowlin, WH Ozel, E Mali, I Episcopo, D Jones, MC Forstner, MRJ AF Brown, Donald J. Nowlin, Weston H. Ozel, Emrah Mali, Ivana Episcopo, Dionne Jones, Melissa C. Forstner, Michael R. J. TI Comparison of short term low, moderate, and high severity fire impacts to aquatic and terrestrial ecosystem components of a southern USA mixed pine/hardwood forest SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Arthropods; Forest; Prescribed fire; Vegetation; Water chemistry; Wildfire ID ENDANGERED HOUSTON TOAD; PINE-GRASSLAND COMMUNITIES; MECHANICAL FUEL REDUCTION; UPLAND HARDWOOD FOREST; PRESCRIBED FIRE; UNITED-STATES; PACIFIC-NORTHWEST; CENTRAL TEXAS; ECOLOGICAL RESTORATION; BUFO-HOUSTONENSIS AB Historically fire was an important natural disturbance shaping the structure and composition of pine-dominated forests in the southern United States. Longstanding fire suppression policies have resulted in structural and compositional changes, notably accumulation of heavy fuel loads and reduction in vegetation species diversity. Primary goals of forest management through prescribed burning include fuel load reduction and mimicking ecosystem impacts of historically natural wildfires. In addition to the influences of fire frequency and season, the influence of fire severity on ecosystem responses is currently of interest. In this study we sought to quantify the impacts of low, moderate, and high severity fires, and their interaction with prior forest management practices, to several aquatic and terrestrial ecosystem components of a southern U.S. mixed pine/hardwood forest using a before-after, control-impact (BACI) approach. The ecosystem components we assessed were water quality, community composition of aquatic arthropods (wildfire impacts only), forest structure characteristics, community composition of understory vegetation, and community composition of ground-dwelling arthropods. We found that increasing fire severity increased aquatic nutrient levels and productivity, but the magnitude of effects increased with severity. Low and moderate severity fires had weak effects on forest structure characteristics, community composition of understory vegetation, and community composition of ground-dwelling arthropods in the initial years following burns. In contrast, high severity fires dramatically reduced fine and large fuel loads, increased diversity of understory vegetation, and influenced community composition of ground-dwelling arthropods. Further, wildfire severity was reduced in areas with a prior moderate severity prescribed burn, but not in areas with a prior low severity prescribed burn. Our results provide quantitative evidence for the role of fire severity as a primary factor influencing responses of ecosystems to fire, and indicate that forest management practices influence the impact of high severity fires on ecosystems. (C) 2013 Elsevier B.V. All rights reserved. C1 [Brown, Donald J.; Ozel, Emrah; Mali, Ivana; Episcopo, Dionne; Jones, Melissa C.; Forstner, Michael R. J.] Texas State Univ, Dept Biol, San Marcos, TX 78666 USA. [Nowlin, Weston H.] Texas State Univ, Dept Biol, Aquat Stn, San Marcos, TX 78666 USA. RP Brown, DJ (reprint author), US Forest Serv, USDA, Inst Appl Ecosyst Studies, No Res Stn, 5985 Cty Highway K, Rhinelander, WI 54501 USA. EM djb.ecology@gmail.com FU Texas Parks and Wildlife Department; U. S. Fish and Wildlife Service FX We thank J. Becker and P. Diaz for assistance with aquatic habitat sampling and analyses, and D. Lemke and J. Angermeier for assistance with plant identification. We thank B. DeVolld, A. Harper, M. Jones, A. Bosworth, A. Gomez, A. Schultz, M. Ray, C. Wiggins, K. Jamerson, E. Feichtinger, S. Dolino, J. Lattanzio, J. Shaw, J. Vila, R. Bogan, J. Barnett, A. Duarte, K. Wooten, M. Heather, S. Uthuppuru, and D. Wallace for assistance with arthropod identification, sampling, or sorting. We thank Jay and Joshua Kane, T. Schumann, M. Sanders, J. Chenoweth, B. Armstrong, and L. Kothmann for assistance with prescribed burns. We thank the Texas Forest Service and local fire departments for assistance with controlling the wildfires. We appreciate T. Swannack for reviewing an earlier draft of this manuscript. Two anonymous reviewers provided suggestions that improved the quality of this manuscript. This study was supported by the Texas Parks and Wildlife Department and U. S. Fish and Wildlife Service through a traditional Section 6 grant. The Capitol Area Council of the Boy Scouts of America provided access to the Griffith League Ranch, and we appreciate their continuing support of our research. NR 109 TC 7 Z9 9 U1 3 U2 62 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JAN 15 PY 2014 VL 312 BP 179 EP 192 DI 10.1016/j.foreco.2013.10.006 PG 14 WC Forestry SC Forestry GA 277EV UT WOS:000328802700020 ER PT J AU Kool, D Agam, N Lazarovitch, N Heitman, JL Sauer, TJ Ben-Gal, A AF Kool, D. Agam, N. Lazarovitch, N. Heitman, J. L. Sauer, T. J. Ben-Gal, A. TI A review of approaches for evapotranspiration partitioning SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Review DE Evaporation; Transpiration; Sparse canopy; Water use efficiency; Energy balance; Water balance ID SOIL-WATER EVAPORATION; SAP FLOW MEASUREMENT; HEAT-PULSE METHOD; WINTER-WHEAT FIELD; PLANT CANOPY MODEL; BOWEN-RATIO SYSTEM; ENERGY-BALANCE; EDDY-COVARIANCE; STABLE-ISOTOPES; BARE SOIL AB Partitioning of evapotranspiration (ET) into evaporation from the soil (E) and transpiration through the stomata of plants (T) is challenging but important in order to assess biomass production and the allocation of increasingly scarce water resources. Generally, T is the desired component with the water being used to enhance plant productivity; whereas, E is considered a source of water loss or inefficiency. The magnitude of E is expected to be quite significant in sparsely vegetated systems, particularly in dry areas or in very wet systems such as surface irrigated crops and wetlands. In these cases, ET partitioning is fundamental to accurately monitor system hydrology and to improve water management practices. This paper aims to evaluate and summarize available methods currently used to separately determine E and T components. We presuppose that, to test the accuracy of ET partitioning methods (measurements and/or modeling), all three components, i.e., E, T and ET, must be estimated independently, but recognize that sometimes one of the components is taken as the residual of the other two. Models that were validated against measurements for their ability to partition between E and Tare briefly discussed. To compare approaches, 52 ET partitioning studies were considered regarding estimates of the relative amount of E and for success of agreement in closing the ET = E+ T equation. The E/ET ratio was found to exceed 30% in 32 of the studies, which confirms the hypothesis that E often constitutes a large fraction of ET and deserves independent consideration. Only 20 studies estimated E and T as well as ET, and had varied results. A number of studies succeeded to estimate E+ T to within 10% of measured ET. Future challenges include development of models simulating the components of ET separately and advancement of methods for continuous measurement of E, T and/or the ratio between the two. (C) 2013 Elsevier B.V. All rights reserved. C1 [Kool, D.; Agam, N.; Lazarovitch, N.] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, French Associates Inst Agr & Biotechnol Drylands, Wyler Dept Dryland Agr, IL-84990 Sede Boqer, Israel. [Kool, D.; Ben-Gal, A.] Gilat Res Ctr, Agr Res Org, Inst Soil Water & Environm Sci, IL-85280 Gilat, Israel. [Heitman, J. L.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Sauer, T. J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Agam, N (reprint author), Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, French Associates Inst Agr & Biotechnol Drylands, Wyler Dept Dryland Agr, Sede Boger Campus, IL-84990 Sede Boqer, Israel. EM agam@bgu.ac.il RI Kool, Dilia/F-8273-2015; Agam, Nurit/E-1836-2013; OI Kool, Dilia/0000-0002-8332-1429; Agam, Nurit/0000-0002-8921-6179; Ben-Gal, Alon/0000-0003-4105-7807 NR 176 TC 63 Z9 68 U1 40 U2 261 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JAN 15 PY 2014 VL 184 BP 56 EP 70 DI 10.1016/j.agrformet.2013.09.003 PG 15 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 275HH UT WOS:000328666400006 ER PT J AU Zhang, LX Allen, LH Vaughan, MM Hauser, BA Boote, KJ AF Zhang, Lingxiao Allen, Leon Hartwell, Jr. Vaughan, Martha M. Hauser, Bernard A. Boote, Kenneth J. TI Solar ultraviolet radiation exclusion increases soybean internode lengths and plant height SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Greenhouse spectral transmission; lnternode elongation; Soybean; Ultraviolet (UV) radiation ID UV-B RADIATION; BLUE-LIGHT RECEPTORS; HIGH-PRESSURE SODIUM; SUPPLEMENTAL BLUE; VEGETATIVE GROWTH; GIBBERELLIC-ACID; CYTOCHROME-P450 MONOOXYGENASE; PHOTOSYNTHETIC ACTIVITIES; COVERING MATERIALS; EMITTING-DIODES AB Soybean [Glycine max (L) Men.] cultivars Williams-82 and Maverick were grown in a polycarbonate greenhouse (P-GH), a glass greenhouse (G-GH), and outdoors during daytime (control) to investigate the effect of (i) exclusion of both ultraviolet B (UV-B, 280-320 nm) and ultraviolet A (UV-A, 320-400 nm), (ii) exclusion of UV-B only, and (iii) exposure to daytime solar UV radiation (09:00-17:00 h local time daily). Both the P-GH and the G-GH were maintained at day/night air temperatures of 30/22 degrees C. The purpose was to quantify the growth differences due to partial and total UV exclusion compared with the control. Exclusion of both UV-B and UV-A radiation caused elongated internodes on the plants, which resulted in greater plant height. Mean mainstem length of Williams-82 was 50.2 cm for the control, but they were 45% and 237% greater for excluded UV-B radiation only and both excluded UV-B and UV-A radiation, respectively. Similarly, mean mainstem length of Maverick was 55.2 cm for the control, but they were 52% and 198% greater for excluded UV-B only and both excluded UV-B and UV-A, respectively. There also was a slight increase in the number of nodes with increasing extent of UV radiation exclusion. The mean final V-stage of Williams-82 was 14.2 for the control, but they were 11% and 18% greater for excluded UV-B only and both excluded UV-B and UV-A, respectively. Likewise, the mean final V-stage of Maverick was 14.5 for the control, but they were 12% and 22% greater for excluded UV-B only and both excluded UV-B and UV-A, respectively. With exclusion of both UV-B and UV-A, pod yield for the Maverick cultivar was greater but this effect was not as clear for the Williams-82 cultivar. In summary, most of the total UV-exclusion effects were due to the exclusion of the UV-A radiation component. Finally, UV spectral transmission of greenhouse or controlled environment covering material should be considered before conducting research on plants, or producing plants commercially, that are sensitive to UV exclusion, such as certain grain legumes or horticultural crops. Published by Elsevier B.V. C1 [Zhang, Lingxiao; Allen, Leon Hartwell, Jr.; Vaughan, Martha M.] ARS, USDA, Gainesville, FL 32608 USA. [Hauser, Bernard A.] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Boote, Kenneth J.] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. RP Allen, LH (reprint author), USDA ARS, Chem Res Unit, CMAVE, Gainesville, FL 32608 USA. EM hartwell.allen@ars.usda.gov OI Boote, Kenneth/0000-0002-1358-5496 FU National Research Initiative of the USDA Cooperative State Research, Education and Extension Service grant [2008-35100-19244]; University of Florida Agricultural Experiment Station; Center for Medical, Agricultural, and Veterinary Entomology, ARS, U.S. Department of Agriculture FX We thank the University of Florida IFAS Facilities, Planning, and Operations team, especially Frank Tipton and Joe Hayden, for development of the controlled environment facility; and Maritza Romero and John Truett for developing data acquisitions systems. We also thank Maritza Romero, John Truett, Emily Wang, and Barth Gervelis for help during the experiments. This research was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service grant to the University of Florida (grant number 2008-35100-19244), the University of Florida Agricultural Experiment Station, and the Center for Medical, Agricultural, and Veterinary Entomology, ARS, U.S. Department of Agriculture. NR 69 TC 10 Z9 10 U1 0 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JAN 15 PY 2014 VL 184 BP 170 EP 178 DI 10.1016/j.agrformet.2013.09.011 PG 9 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 275HH UT WOS:000328666400015 ER PT J AU Anderson, RG Wang, D AF Anderson, Ray G. Wang, Dong TI Energy budget closure observed in paired Eddy Covariance towers with increased and continuous daily turbulence SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Eddy Covariance; Energy budgets; Turbulence; Methods ID NET ECOSYSTEM EXCHANGE; SUB-ALPINE FOREST; BALANCE CLOSURE; CARBON-DIOXIDE; FLUX MEASUREMENTS; HIGH-ELEVATION; BOREAL FOREST; SENSIBLE HEAT; WATER-VAPOR; EVAPOTRANSPIRATION AB The lack of energy closure has been a longstanding issue with Eddy Covariance (EC). Multiple mechanisms have been proposed to explain the discrepancies in energy balance including diurnal energy storage changes, advection of energy, and larger scale turbulent processes that cannot be resolved by field EC. To investigate the energy balance issue, we used a year of data from paired EC towers in irrigated sugarcane in Maui, Hawai'i, USA. The towers were in identical crops and cultivation practices and had similar climate with the notable exception of wind. One tower was in a location where nearby orographic features funneled Trade Winds, resulting in sustained, continuous turbulence. The other was in a leeward location with less turbulence, particularly at night (u*). We found significantly improved closure (8.5-10%) at both sites using daily sums of Available Energy in closure regressions as opposed to 30 min data, illustrating the importance of storage terms. The energy budget closed for both fields when only days with continuous turbulence (all 30 min u*> critical u*) were considered, with significantly larger uncertainty in the leeward field ( +/- 13%) due to the small number of days (n = 13) with this condition. Significant energy imbalance appeared in both fields with even 30 min of subcritical turbulence in a day, and each field had different turbulence-closure patterns. Closure with continuous turbulence was sensitive to choice of critical if; an arbitrary u* of 0.1 ms(-1) resulted in non-closure. The results show the value of paired EC towers in contrasting turbulence conditions to assess energy budget closure. Published by Elsevier B.V. C1 [Anderson, Ray G.; Wang, Dong] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Water Management Res Unit, Parlier, CA 93648 USA. [Anderson, Ray G.] ARS, USDA, US Salin Lab, Contaminant Fate & Transport Unit, Riverside, CA 92507 USA. RP Anderson, RG (reprint author), USDA ARS, George E Brown Jr Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM ray.anderson@ars.usda.gov; dong.wang@ars.usda.gov OI Anderson, Ray/0000-0002-6202-5890 FU U.S. Navy, Office of Naval Research FX We thank Mae Nakahata for providing research access and assistance. Jim Gartung, ARS-Parlier, assisted with the establishment of the EC tower. Adel Youkhana, Neil Abranyi, and Jason Drogowski assisted with field support. This research was partially supported by the U.S. Navy, Office of Naval Research. NR 50 TC 14 Z9 15 U1 1 U2 42 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JAN 15 PY 2014 VL 184 BP 204 EP 209 DI 10.1016/j.agrformet.2013.09.012 PG 6 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 275HH UT WOS:000328666400018 ER PT J AU Balsam, J Rasooly, R Bruck, HA Rasooly, A AF Balsam, Joshua Rasooly, Reuven Bruck, Hugh Alan Rasooly, Avraham TI Thousand-fold fluorescent signal amplification for mHealth diagnostics SO BIOSENSORS & BIOELECTRONICS LA English DT Article DE Lab-on-a-chip; mHealth; Capillary; Fluorescence; Image stacking; Mobile phone ID CELL-PHONE; BIOSENSOR; IMMUNOSENSOR; IMMUNOASSAY; PLATFORM; HEALTH; SENSOR; POINT AB The low sensitivity of Mobile Health (mHealth) optical detectors, such as those found on mobile phones, is a limiting factor for many mHealth clinical applications. To improve sensitivity, we have combined two approaches for optical signal amplification: (1) a computational approach based on an image stacking algorithm to decrease the image noise and enhance weak signals, and (2) an optical signal amplifier utilizing a capillary tube array. These approaches were used in a detection system which includes multi-wavelength LEDs capable of exciting many fluorophores in multiple wavelengths, a mobile phone or a webcam as a detector, and capillary tube array configured with 36 capillary tubes for signal enhancement. The capillary array enables a similar to 100 x increase in signal sensitivity for fluorescein, reducing the limit of detection (LOD) for mobile phones and webcams from 1000 nM to 10 nM. Computational image stacking enables another similar to 10 x increase in signal sensitivity, further reducing the LOD for webcam from 10 nM to 1 nM. To demonstrate the feasibility of the device for the detection of disease-related biomarkers, adenovirus DNA labeled with SYBR green or fluorescein was analyzed by both our capillary array and a commercial plate reader. The LOD for the capillary array was 5 ug/mL, and that of the plate reader was 1 ug/mL. Similar results were obtained using DNA stained with fluorescein. The combination of the two signal amplification approaches enables a similar to 1000 x increase in LOD for the webcam platform. This brings it into the range of a conventional plate reader while using a smaller sample volume (10 ul) than the plate reader requires (100 ul). This suggests that such a device could be suitable for biosensing applications where up to 10 fold smaller sample sizes are needed. The simple optical configuration for mHealth described in this paper employing the combined capillary and image processing signal amplification is capable of measuring weak fluorescent signals without the need of dedicated laboratories. It has the potential to be used to increase sensitivity of other optically based mHealth technologies, and may increase mHealth's clinical utility, especially for telemedicine and for resource-poor settings and global health applications. Published by Elsevier B.V. C1 [Balsam, Joshua; Rasooly, Avraham] FDA, Div Biol, Off Sci & Engn, Silver Spring, MD 20993 USA. [Balsam, Joshua; Bruck, Hugh Alan] Univ Maryland, College Pk, MD 20742 USA. [Rasooly, Reuven] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Rasooly, Avraham] NCI, Div Canc Biol, Bethesda, MD 20892 USA. RP Rasooly, A (reprint author), FDA, Div Biol, Off Sci & Engn, Silver Spring, MD 20993 USA. EM rasoolya@mail.nih.gov FU FDA's Center for Devices and Radiological Health, Division of Biology; National Cancer Institute FX This work was supported by the FDA's Center for Devices and Radiological Health, Division of Biology and the National Cancer Institute. The views expressed are those of the authors and do not represent those of the U.S. Government. NR 35 TC 9 Z9 9 U1 5 U2 84 PU ELSEVIER ADVANCED TECHNOLOGY PI OXFORD PA OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-5663 EI 1873-4235 J9 BIOSENS BIOELECTRON JI Biosens. Bioelectron. PD JAN 15 PY 2014 VL 51 BP 1 EP 7 DI 10.1016/j.bios.2013.06.053 PG 7 WC Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology SC Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics GA 251CN UT WOS:000326905500001 PM 23928092 ER PT J AU Proestou, DA Flight, P Champlin, D Nacci, D AF Proestou, Dina A. Flight, Patrick Champlin, Denise Nacci, Diane TI Targeted approach to identify genetic loci associated with evolved dioxin tolerance in Atlantic Killifish (Fundulus heteroclitus) SO BMC EVOLUTIONARY BIOLOGY LA English DT Article DE Adaptation; Ecotoxicology; Candidate gene scan; Killifish ID NATURAL-POPULATIONS; AROMATIC-HYDROCARBONS; POLLUTION TOLERANCE; RECEPTOR; ADAPTATION; EXPRESSION; SENSITIVITY; RESISTANCE; DIVERSITY; ZEBRAFISH AB Background: The most toxic aromatic hydrocarbon pollutants are categorized as dioxin-like compounds (DLCs) to which extreme tolerance has evolved independently and contemporaneously in (at least) four populations of Atlantic killifish (Fundulus heteroclitus). Surprisingly, the magnitude and phenotype of DLC tolerance is similar among these killifish populations that have adapted to varied, but highly aromatic hydrocarbon-contaminated urban/industrialized estuaries of the US Atlantic coast. Multiple tolerant and neighboring sensitive killifish populations were compared with the expectation that genetic loci associated with DLC tolerance would be revealed. Results: Since the aryl hydrocarbon receptor (AHR) pathway partly or fully mediates DLC toxicity in vertebrates, single nucleotide polymorphisms (SNPs) from 42 genes associated with the AHR pathway were identified to serve as targeted markers. Wild fish (N = 36/37) from four highly tolerant killifish populations and four nearby sensitive populations were genotyped using 59 SNP markers. Similar to other killifish population genetic analyses, strong genetic differentiation among populations was detected, consistent with isolation by distance models. When DLC-sensitive populations were pooled and compared to pooled DLC-tolerant populations, multi-locus analyses did not distinguish the two groups. However, pairwise comparisons of nearby tolerant and sensitive populations revealed high differentiation among sensitive and tolerant populations at these specific loci: AHR 1 and 2, cathepsin Z, the cytochrome P450s (CYP1A and 3A30), and the NADH dehydrogenase subunits. In addition, significant shifts in minor allele frequency were observed at AHR2 and CYP1A loci across most sensitive/tolerant pairs, but only AHR2 exhibited shifts in the same direction across all pairs. Conclusions: The observed differences in allelic composition at the AHR2 and CYP1A SNP loci were identified as significant among paired sensitive/tolerant populations of Atlantic killifish with multiple statistical tests. The genetic patterns reported here lend support to the argument that AHR2 and CYP1A play a role in the adaptive response to extreme DLC contamination. Additional functional assays are required to isolate the exact mechanism of DLC tolerance. C1 [Proestou, Dina A.; Champlin, Denise; Nacci, Diane] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Atlantic Ecol Div, Narragansett, RI 02882 USA. [Flight, Patrick] Brown Univ, Dept Ecol & Evolutionary Biol, Providence, RI 02912 USA. RP Proestou, DA (reprint author), ARS, USDA, Ctr Biotechnol & Life Sci 469, 120 Flagg Rd, Kingston, RI 02881 USA. EM dina.proestou@ars.usda.gov NR 59 TC 12 Z9 12 U1 2 U2 24 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2148 J9 BMC EVOL BIOL JI BMC Evol. Biol. PD JAN 14 PY 2014 VL 14 AR 7 DI 10.1186/1471-2148-14-7 PG 17 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA AI3LU UT WOS:000336763800001 PM 24422627 ER PT J AU Tyler, L Fangel, JU Fagerstrom, AD Steinwand, MA Raab, TK Willats, WGT Vogel, JP AF Tyler, Ludmila Fangel, Jonatan U. Fagerstrom, Alexandra Dotson Steinwand, Michael A. Raab, Theodore K. Willats, William G. T. Vogel, John P. TI Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines SO BMC PLANT BIOLOGY LA English DT Article DE Brachypodium distachyon; Cell wall; NIR; Seed; Biofuel ID AGROBACTERIUM-MEDIATED TRANSFORMATION; CELL-WALL; MODEL SYSTEM; GRASSES; SPECTROSCOPY; BIOMASS; FIBER; STEMS AB Background: The model grass Brachypodium distachyon is increasingly used to study various aspects of grass biology. A large and genotypically diverse collection of B. distachyon germplasm has been assembled by the research community. The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits. Results: We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops. Phenotypic characters examined included plant height, growth habit, stem density, flowering time, and seed weight. We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP). In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass. Conclusion: These characterizations can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation. C1 [Tyler, Ludmila; Steinwand, Michael A.; Vogel, John P.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Fangel, Jonatan U.; Fagerstrom, Alexandra Dotson; Willats, William G. T.] Univ Copenhagen, Copenhagen, Denmark. [Raab, Theodore K.] Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA. RP Vogel, JP (reprint author), Univ Calif Berkeley, Berkeley, CA 94720 USA. EM john.vogel@ars.usda.gov OI Vogel, John/0000-0003-1786-2689; Willats, William/0000-0003-2064-4025; Raab, Theodore K./0000-0002-3965-8729 FU Office of Science (BER); US Department of Energy; USDA CRIS [5325-21000-017-00] FX We would like to thank Matthew De La Housaye for technical assistance. This work was supported by the Office of Science (BER), US Department of Energy, and by USDA CRIS project 5325-21000-017-00. NR 37 TC 14 Z9 15 U1 0 U2 32 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD JAN 14 PY 2014 VL 14 AR 25 DI 10.1186/1471-2229-14-25 PG 15 WC Plant Sciences SC Plant Sciences GA AF1JU UT WOS:000334471000001 PM 24423101 ER PT J AU Calingacion, M Laborte, A Nelson, A Resurreccion, A Concepcion, JC Daygon, VD Mumm, R Reinke, R Dipti, S Bassinello, PZ Manful, J Sophany, S Lara, KC Bao, JS Xie, LH Loaiza, K El-hissewy, A Gayin, J Sharma, N Rajeswari, S Manonmani, S Rani, NS Kota, S Indrasari, SD Habibi, F Hosseini, M Tavasoli, F Suzuki, K Umemoto, T Boualaphanh, C Lee, HH Hung, YP Ramli, A Aung, PP Ahmad, R Wattoo, JI Bandonill, E Romero, M Brites, CM Hafeel, R Lur, HS Cheaupun, K Jongdee, S Blanco, P Bryant, R Lang, NT Hall, RD Fitzgerald, M AF Calingacion, Mariafe Laborte, Alice Nelson, Andrew Resurreccion, Adoracion Concepcion, Jeanaflor Crystal Daygon, Venea Dara Mumm, Roland Reinke, Russell Dipti, Sharifa Bassinello, Priscila Zaczuk Manful, John Sophany, Sakhan Lara, Karla Cordero Bao, Jinsong Xie, Lihong Loaiza, Katerine El-hissewy, Ahmad Gayin, Joseph Sharma, Neerja Rajeswari, Sivakami Manonmani, Swaminathan Rani, N. Shobha Kota, Suneetha Indrasari, Siti Dewi Habibi, Fatemeh Hosseini, Maryam Tavasoli, Fatemeh Suzuki, Keitaro Umemoto, Takayuki Boualaphanh, Chanthkone Lee, Huei Hong Hung, Yiu Pang Ramli, Asfaliza Aung, Pa Pa Ahmad, Rauf Wattoo, Javed Iqbal Bandonill, Evelyn Romero, Marissa Brites, Carla Moita Hafeel, Roshni Lur, Huu-Sheng Cheaupun, Kunya Jongdee, Supanee Blanco, Pedro Bryant, Rolfe Nguyen Thi Lang Hall, Robert D. Fitzgerald, Melissa TI Diversity of Global Rice Markets and the Science Required for Consumer-Targeted Rice Breeding SO PLOS ONE LA English DT Article ID SINGLE-NUCLEOTIDE POLYMORPHISMS; ORYZA-SATIVA L.; STARCH-SYNTHASE-IIA; GRAIN LENGTH; GELATINIZATION TEMPERATURE; GEL CONSISTENCY; EATING QUALITY; GENE; GS3; ASSOCIATION AB With the ever-increasing global demand for high quality rice in both local production regions and with Western consumers, we have a strong desire to understand better the importance of the different traits that make up the quality of the rice grain and obtain a full picture of rice quality demographics. Rice is by no means a 'one size fits all' crop. Regional preferences are not only striking, they drive the market and hence are of major economic importance in any rice breeding/improvement strategy. In this analysis, we have engaged local experts across the world to perform a full assessment of all the major rice quality trait characteristics and importantly, to determine how these are combined in the most preferred varieties for each of their regions. Physical as well as biochemical characteristics have been monitored and this has resulted in the identification of no less than 18 quality trait combinations. This complexity immediately reveals the extent of the specificity of consumer preference. Nevertheless, further assessment of these combinations at the variety level reveals that several groups still comprise varieties which consumers can readily identify as being different. This emphasises the shortcomings in the current tools we have available to assess rice quality and raises the issue of how we C1 [Calingacion, Mariafe; Laborte, Alice; Nelson, Andrew; Resurreccion, Adoracion; Concepcion, Jeanaflor Crystal; Daygon, Venea Dara; Fitzgerald, Melissa] Int Rice Res Inst, Los Banos, Laguna, Philippines. [Calingacion, Mariafe] Wageningen Univ, Lab Plant Physiol, NL-6700 AP Wageningen, Netherlands. [Calingacion, Mariafe; Resurreccion, Adoracion; Concepcion, Jeanaflor Crystal; Daygon, Venea Dara; Reinke, Russell; Dipti, Sharifa; Bassinello, Priscila Zaczuk; Manful, John; Sophany, Sakhan; Lara, Karla Cordero; Bao, Jinsong; Xie, Lihong; Loaiza, Katerine; El-hissewy, Ahmad; Gayin, Joseph; Sharma, Neerja; Rajeswari, Sivakami; Manonmani, Swaminathan; Rani, N. Shobha; Kota, Suneetha; Indrasari, Siti Dewi; Habibi, Fatemeh; Hosseini, Maryam; Tavasoli, Fatemeh; Suzuki, Keitaro; Umemoto, Takayuki; Boualaphanh, Chanthkone; Lee, Huei Hong; Ramli, Asfaliza; Aung, Pa Pa; Ahmad, Rauf; Wattoo, Javed Iqbal; Bandonill, Evelyn; Romero, Marissa; Brites, Carla Moita; Hafeel, Roshni; Lur, Huu-Sheng; Cheaupun, Kunya; Jongdee, Supanee; Blanco, Pedro; Bryant, Rolfe; Nguyen Thi Lang; Hall, Robert D.; Fitzgerald, Melissa] Int Network Qual Rice, Wageningen, Netherlands. [Mumm, Roland; Hall, Robert D.] Plant Res Int, Wageningen, Netherlands. [Mumm, Roland; Hall, Robert D.] Ctr BioSyst Genom, Wageningen, Netherlands. [Reinke, Russell] NSW Dept Ind & Investment, Yanco Agr Inst, Yanco, NSW, Australia. [Dipti, Sharifa] Bangladesh Rice Res Inst, Grain Qual & Nutr Div, Gazipur, Bangladesh. [Bassinello, Priscila Zaczuk] EMBRAPA Rice & Beans, Santo Antonio De Goias, Go, Brazil. [Manful, John] Africa Rice Ctr AfricaRice, Cotonou, Benin. [Sophany, Sakhan] Cambodian Agr Res & Dev Inst, Phnom Penh, Cambodia. [Lara, Karla Cordero] Mejoramiento Genet Arroz INIA CRI Quilamapu, Vicente Mendez, Chile. [Bao, Jinsong] Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Zhejiang, Peoples R China. [Xie, Lihong] China Natl Rice Res Inst, Hangzhou, Zhejiang, Peoples R China. [Loaiza, Katerine] CIAT, Lab Calidad FLAR CIAT, Cali, Colombia. [El-hissewy, Ahmad] Rice Res & Training Ctr, ARC, Rice Res Sect, Field Crops Res Inst, Sakha, Kafr El Shiekh, Egypt. [Gayin, Joseph] CSIR Food Res Inst, Accra, Ghana. [Sharma, Neerja] Punjab Agr Univ, Dept Plant Breeding & Genet, Rice Sect, Ludhiana 141004, Punjab, India. [Rajeswari, Sivakami; Manonmani, Swaminathan] Tamil Nadu Agr Univ, Ctr Plant Breeding & Genet, Dept Rice, Coimbatore 641003, Tamil Nadu, India. [Rani, N. Shobha; Kota, Suneetha] Directorate Rice Res, Crop Improvement Sect, Hyderabad, Andhra Pradesh, India. [Indrasari, Siti Dewi] Indonesian Ctr Rice Res ICRR BB Padi, Sukamandi, Subang Jawa Bar, Indonesia. [Habibi, Fatemeh; Hosseini, Maryam; Tavasoli, Fatemeh] Rice Res Inst Iran, Rasht, Iran. [Suzuki, Keitaro] NARO Inst Crop Sci, Tsukuba, Ibaraki, Japan. [Umemoto, Takayuki] NARO Hokkaido Agr Res Ctr, Sapporo, Hokkaido, Japan. [Boualaphanh, Chanthkone] NAFRI, Rice & Cash Crop Res Inst, Viangchan, Lao PDR, Laos. [Lee, Huei Hong; Hung, Yiu Pang] Univ Putra Malaysia, Fac Agr & Food Sci, Bintulu Sarawak, Malaysia. [Ramli, Asfaliza] MARDI Seberang Perai Beg Berkunci, Pusat Penyelidikan Padi Dan Tanaman Ind, Seberang Perai Pulau Pen, Malaysia. [Aung, Pa Pa] Minist Agr & Irrigat, Myanmar Agr Serv, Ctr Plant Biotechnol, Yangon, Myanmar. [Ahmad, Rauf] Natl Agr Res Ctr, Rice Programme, Islamabad, Pakistan. [Wattoo, Javed Iqbal] Natl Inst Biotechnol & Genet Engn, Faislabad, Pakistan. [Bandonill, Evelyn; Romero, Marissa] Philippine Rice Res Inst, Rice Chem & Food Sci Div, Sci City Munoz, Nueva Ecija, Philippines. [Brites, Carla Moita] Inst Nacl Invest Agr & Vet, Oeiras, Portugal. [Hafeel, Roshni] Dept Agr, Rice Res Stn, Ambalantota, Sri Lanka. [Lur, Huu-Sheng] Natl Taiwan Univ, Dept Agron, Taipei, Taiwan. [Jongdee, Supanee] Khon Kaen Rice Res Ctr, Khon Kaen, Thailand. [Blanco, Pedro] INIA Treinta Tres, Natl Agr Res Inst, Rice Res Program, Treinta Tres, CP, Uruguay. [Bryant, Rolfe] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Nguyen Thi Lang] Cuulong Delta Rice Res Inst, Genet & Plant Breeding Div, Can Tho, Vietnam. RP Fitzgerald, M (reprint author), Int Rice Res Inst, Los Banos, Laguna, Philippines. EM m.fitzgerald2@uq.edu.au RI Bao, Jinsong/A-7413-2008; Brites, Carla/C-4792-2009; hall, robert/B-8707-2015; Nelson, Andrew/G-3649-2012; OI Brites, Carla/0000-0003-1949-172X; Nelson, Andrew/0000-0002-7249-3778; Daygon, Venea Dara/0000-0003-0805-5148; Calingacion, Mariafe/0000-0002-7560-2036 FU International Network for Quality Rice (INQR); International Rice Research Institute (IRRI); Wageningen University Research (WUR); Monsanto Beachell Borlaug Scholarship Foundation FX This work was supported by International Network for Quality Rice (INQR), International Rice Research Institute (IRRI), Wageningen University Research (WUR), and the Monsanto Beachell Borlaug Scholarship Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 32 Z9 36 U1 5 U2 42 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 14 PY 2014 VL 9 IS 1 AR e85106 DI 10.1371/journal.pone.0085106 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 292TQ UT WOS:000329925800022 PM 24454799 ER PT J AU Calkin, DE Cohen, JD Finney, MA Thompson, MP AF Calkin, David E. Cohen, Jack D. Finney, Mark A. Thompson, Matthew P. TI How risk management can prevent future wildfire disasters in the wildland-urban interface SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE risk assessment; fuel treatment; home ignition zone ID UNITED-STATES; FIRE SEVERITY; SIERRA-NEVADA; FORESTS; USA; CALIFORNIA; AREA AB Recent fire seasons in the western United States are some of the most damaging and costly on record. Wildfires in the wildland-urban interface on the Colorado Front Range, resulting in thousands of homes burned and civilian fatalities, although devastating, are not without historical reference. These fires are consistent with the characteristics of large, damaging, interface fires that threaten communities across much of the western United States. Wildfires are inevitable, but the destruction of homes, ecosystems, and lives is not. We propose the principles of risk analysis to provide land management agencies, first responders, and affected communities who face the inevitability of wildfires the ability to reduce the potential for loss. Overcoming perceptions of wildland-urban interface fire disasters as a wildfire control problem rather than a home ignition problem, determined by home ignition conditions, will reduce home loss. C1 [Calkin, David E.; Thompson, Matthew P.] US Forest Serv, Forestry Sci Lab, Rocky Mt Res Stn, Missoula, MT 59807 USA. [Cohen, Jack D.; Finney, Mark A.] US Forest Serv, Fire Sci Lab, Rocky Mt Res Stn, Missoula, MT 59808 USA. RP Calkin, DE (reprint author), US Forest Serv, Forestry Sci Lab, Rocky Mt Res Stn, Missoula, MT 59807 USA. EM decalkin@fs.fed.us RI Calkin, David/F-4727-2014 NR 48 TC 39 Z9 43 U1 2 U2 52 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN 14 PY 2014 VL 111 IS 2 BP 746 EP 751 DI 10.1073/pnas.1315088111 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 288LW UT WOS:000329614500047 PM 24344292 ER PT J AU Bruce, M Neugebauer, KA Joly, DL Migeon, P Cuomo, CA Wang, SC Akhunov, E Bakkeren, G Kolmer, JA Fellers, JP AF Bruce, Myron Neugebauer, Kerri A. Joly, David L. Migeon, Pierre Cuomo, Christina A. Wang, Shichen Akhunov, Eduard Bakkeren, Guus Kolmer, James A. Fellers, John P. TI Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Puccinia triticina; secreted peptides; effectors; leaf rust; RNA sequencing ID LEAF RUST; MAGNAPORTHE-GRISEA; CERATO-PLATANIN; PLANT-CELLS; PROTEINS; RESISTANCE; VIRULENCE; GENOME; FUNGI; INHERITANCE AB Wheat leaf rust, caused by the basidiomycete Puccinia triticina, can cause yield losses of up to 20% in wheat producing regions. During infection, the fungus forms haustoria that secrete proteins into the plant cell and effect changes in plant transcription, metabolism, and defense. It is hypothesized that new races emerge as a result of overcoming plant resistance via changes in the secreted effector proteins. To understand gene expression during infection and find genetic differences associated with races, RNA from wheat leaves infected with six different rust races, at 6 days post inoculation, was sequenced using Illumina. As P triticina is an obligate biotroph, RNA from both the host and fungi were present and separated by alignment to the P triticina genome and a wheat EST reference. A total of 222,571 rust contigs were assembled from 165 million reads. An examination of the resulting contigs revealed 532 predicted secreted proteins among the transcripts. Of these, 456 were found in all races. Fifteen genes were found with amino acid changes, corresponding to putative avirulence effectors potentially recognized by 11 different leaf rust resistance (Lr) genes. Twelve of the potential avirulence effectors have no homology to known genes. One gene had significant similarity to cerato-platanin, a known fungal elicitor, and another showed similarity to fungal tyrosinase, an enzyme involved in melanin synthesis. Temporal expression profiles were developed for these genes by qRT-PCR and show that the genes expression patterns were consistent between races from infection initiation to just prior to spore eruption. C1 [Bruce, Myron; Fellers, John P.] USDA ARS, Hard Winter Wheat Genet Res Unit, Dept Plant Pathol, Manhattan, KS 66506 USA. [Neugebauer, Kerri A.; Migeon, Pierre; Wang, Shichen; Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Joly, David L.] Univ Moncton, Dept Biol, Moncton, NB E1A 3E9, Canada. [Cuomo, Christina A.] Broad Inst MIT & Harvard, Cambridge, MA USA. [Bakkeren, Guus] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Summerland, BC, Canada. [Kolmer, James A.] Univ Minnesota, USDA ARS, Cereal Dis Lab, Dept Plant Pathol, St Paul, MN 55108 USA. RP Fellers, JP (reprint author), USDA ARS, Dept Plant Pathol, 4008 Throckmorton Hall, Manhattan, KS 66506 USA. EM john.fellers@ars.usda.gov RI Wang, Shichen/E-6213-2012; Joly, David/B-4612-2012; OI Wang, Shichen/0000-0003-1441-2252; Bruce, Myron/0000-0001-9235-7258; Joly, David/0000-0001-8411-5558; Bakkeren, Guus/0000-0002-3065-6989; Cuomo, Christina/0000-0002-5778-960X FU USDA-ARS CRIS [5430-21000-010-00D] FX The authors would like to thank Dr. Robert Bowden for his comments during the preparation of this manuscript. This work was funded through USDA-ARS CRIS 5430-21000-010-00D. This is a joint contribution of the United States Department of Agriculture, Agriculture Research Service and the Kansas Agricultural Experiment Station. Contribution no. 14-114J. NR 32 TC 6 Z9 7 U1 3 U2 33 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JAN 13 PY 2014 VL 4 AR 520 DI 10.3389/fpls.2013.00520 PG 7 WC Plant Sciences SC Plant Sciences GA AB0YH UT WOS:000331517800001 PM 24454317 ER PT J AU Schoebel, CN Stewart, J Gruenwald, NJ Rigling, D Prospero, S AF Schoebel, Corine N. Stewart, Jane Gruenwald, Niklaus J. Rigling, Daniel Prospero, Simone TI Population History and Pathways of Spread of the Plant Pathogen Phytophthora plurivora SO PLOS ONE LA English DT Article ID STATISTICAL PROPERTIES; BIOLOGICAL INVASIONS; SELF-FERTILIZATION; GENETIC DIVERSITY; POWER ANALYSIS; CLIMATE-CHANGE; DNA-SEQUENCES; NORTH-AMERICA; 2 TESTS; FUNGUS AB Human activity has been shown to considerably affect the spread of dangerous pests and pathogens worldwide. Therefore, strict regulations of international trade exist for particularly harmful pathogenic organisms. Phytophthora plurivora, which is not subject to regulations, is a plant pathogen frequently found on a broad range of host species, both in natural and artificial environments. It is supposed to be native to Europe while resident populations are also present in the US. We characterized a hierarchical sample of isolates from Europe and the US and conducted coalescent-, migration, and population genetic analysis of sequence and microsatellite data, to determine the pathways of spread and the demographic history of this pathogen. We found P. plurivora populations to be moderately diverse but not geographically structured. High levels of gene flow were observed within Europe and unidirectional from Europe to the US. Coalescent analyses revealed a signal of a recent expansion of the global P. plurivora population. Our study shows that P. plurivora has most likely been spread around the world by nursery trade of diseased plant material. In particular, P. plurivora was introduced into the US from Europe. International trade has allowed the pathogen to colonize new environments and/or hosts, resulting in population growth. C1 [Schoebel, Corine N.; Rigling, Daniel; Prospero, Simone] Swiss Fed Inst Forest Snow & Landscape Res WSL, Dept Biodivers & Conservat Biol, Birmensdorf, Switzerland. [Stewart, Jane; Gruenwald, Niklaus J.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR USA. [Stewart, Jane; Gruenwald, Niklaus J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Schoebel, CN (reprint author), Swiss Fed Inst Forest Snow & Landscape Res WSL, Dept Biodivers & Conservat Biol, Birmensdorf, Switzerland. EM corine.schoebel@wsl.ch RI Schoebel, Corine/D-2801-2013; OI Schoebel, Corine/0000-0001-9254-140X; Grunwald, Niklaus/0000-0003-1656-7602 FU COST Switzerland [SER C10.0129]; Swiss National Science Foundation FX This study was funded by COST Switzerland (project SER C10.0129) and supported by an international short visit grant of the Swiss National Science Foundation to CNS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 87 TC 12 Z9 12 U1 3 U2 38 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 10 PY 2014 VL 9 IS 1 AR e85368 DI 10.1371/journal.pone.0085368 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 291YG UT WOS:000329868200044 PM 24427303 ER PT J AU Cooper, KK Mandrell, RE Louie, JW Korlach, J Clark, TA Parker, CT Huynh, S Chain, PS Ahmed, S Carter, MQ AF Cooper, Kerry K. Mandrell, Robert E. Louie, Jacqueline W. Korlach, Jonas Clark, Tyson A. Parker, Craig T. Huynh, Steven Chain, Patrick S. Ahmed, Sanaa Carter, Michelle Qiu TI Comparative genomics of enterohemorrhagic Escherichia coli O145:H28 demonstrates a common evolutionary lineage with Escherichia coli O157:H7 SO BMC GENOMICS LA English DT Article DE Comparative genomics; Enterohemorrhagic Escherichia coli; Shiga toxin-producing Escherichia coli; DNA methylation; O145 ID NON-O157 SHIGA TOXIN; HEMOLYTIC-UREMIC SYNDROME; UNITED-STATES; SINGLE-MOLECULE; PARALLEL EVOLUTION; MAXIMUM-LIKELIHOOD; STEPWISE EMERGENCE; DNA METHYLATION; PHASE VARIATION; DIVERSITY AB Background: Although serotype O157:H7 is the predominant enterohemorrhagic Escherichia coli (EHEC), outbreaks of non-O157 EHEC that cause severe foodborne illness, including hemolytic uremic syndrome have increased worldwide. In fact, non-O157 serotypes are now estimated to cause over half of all the Shiga toxin-producing Escherichia coli (STEC) cases, and outbreaks of non-O157 EHEC infections are frequently associated with serotypes O26, O45, O103, O111, O121, and O145. Currently, there are no complete genomes for O145 in public databases. Results: We determined the complete genome sequences of two O145 strains (EcO145), one linked to a US lettuce-associated outbreak (RM13514) and one to a Belgium ice-cream-associated outbreak (RM13516). Both strains contain one chromosome and two large plasmids, with genome sizes of 5,737,294 bp for RM13514 and 5,559,008 bp for RM13516. Comparative analysis of the two EcO145 genomes revealed a large core (5,173 genes) and a considerable amount of strain-specific genes. Additionally, the two EcO145 genomes display distinct chromosomal architecture, virulence gene profile, phylogenetic origin of Stx2a prophage, and methylation profile (methylome). Comparative analysis of EcO145 genomes to other completely sequenced STEC and other E. coli and Shigella genomes revealed that, unlike any other known non-O157 EHEC strain, EcO145 ascended from a common lineage with EcO157/EcO55. This evolutionary relationship was further supported by the pangenome analysis of the 10 EHEC strains. Of the 4,192 EHEC core genes, EcO145 shares more genes with EcO157 than with the any other non-O157 EHEC strains. Conclusions: Our data provide evidence that EcO145 and EcO157 evolved from a common lineage, but ultimately each serotype evolves via a lineage-independent nature to EHEC by acquisition of the core set of EHEC virulence factors, including the genes encoding Shiga toxin and the large virulence plasmid. The large variation between the two EcO145 genomes suggests a distinctive evolutionary path between the two outbreak strains. The distinct methylome between the two EcO145 strains is likely due to the presence of a BsuBI/PstI methyltransferase gene cassette in the Stx2a prophage of the strain RM13514, suggesting a role of horizontal gene transfer-mediated epigenetic alteration in the evolution of individual EHEC strains. C1 [Cooper, Kerry K.; Mandrell, Robert E.; Louie, Jacqueline W.; Parker, Craig T.; Huynh, Steven; Carter, Michelle Qiu] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Korlach, Jonas; Clark, Tyson A.] Pacific Biosci, Menlo Pk, CA USA. [Chain, Patrick S.; Ahmed, Sanaa] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA. RP Carter, MQ (reprint author), ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM michelle.carter@ars.usda.gov OI Chain, Patrick/0000-0003-3949-3634 FU USDA-ARS CRIS [5325-046, 5325-047]; USDA Research Associate Program funds FX The authors thank Anna Bates and Jaszemyn Yambao for their assistance with bacterial cultures; Gerard Lazo and Yong Gu for their assistance with whole-genome based phylogenetic analysis; Matthew Boitano for assistance with PacBio sample preparation; Khai Luong for assistance with data analysis; Denis Pierard (Brussels, Belgium) for providing the Belgian outbreak strain; and James Rudrik (Lansing, MI) for providing the US outbreak strain. We thank Richard Roberts (New England Biolabs) for bioinformatic analysis of the methyltransferase gene assignments and comparison. This research work is supported by USDA-ARS CRIS 5325-046 and -047. KK Cooper was supported by USDA Research Associate Program funds awarded to M. Q. Carter. NR 75 TC 19 Z9 20 U1 1 U2 28 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JAN 10 PY 2014 VL 15 AR 17 DI 10.1186/1471-2164-15-17 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 288MI UT WOS:000329615700001 PM 24410921 ER PT J AU Lin, F Zhao, MX Baumann, DD Ping, JQ Sun, LJ Liu, YF Zhang, B Tang, ZX Hughes, E Doerge, RW Hughes, TJ Ma, JX AF Lin, Feng Zhao, Meixia Baumann, Douglas D. Ping, Jieqing Sun, Lianjun Liu, Yunfeng Zhang, Biao Tang, Zongxiang Hughes, Elisa Doerge, Rebecca W. Hughes, Teresa J. Ma, Jianxin TI Molecular response to the pathogen Phytophthora sojae among ten soybean near isogenic lines revealed by comparative transcriptomics SO BMC GENOMICS LA English DT Article DE Comparative transcriptomics; Resistance to Phytophthora sojae; Soybean ID GENE-EXPRESSION NORMALIZATION; DISEASE-RESISTANCE; CONFERRING RESISTANCE; PSEUDOMONAS-SYRINGAE; DEFENSE RESPONSES; AVIRULENCE GENES; ARABIDOPSIS RIN4; PLANT IMMUNITY; ABSCISIC-ACID; RPS8 MAPS AB Background: Phytophthora root and stem rot (PRR) of soybean, caused by Phytophthora sojae, is controlled by Rps genes. However, little is known regarding the Rps-induced molecular responses to P. sojae and how they actually overlap. We thus sequenced, analyzed, and compared the transcriptomes of 10 near isogenic lines (NILs), each with a unique Rps gene/allele, and the susceptible parent Williams, pre- and post-inoculation with the pathogen. Results: A total of 4,330 differentially expressed genes (DEGs) were identified in Williams versus 2,014 to 5,499 DEGs in individual NILs upon inoculation with the pathogen. Comparisons of the DEGs between the NILs and Williams identified incompatible interaction genes (IIGs) and compatible interaction genes (CIGs). Hierarchical cluster and heatmap analyses consistently grouped the NILs into three clusters: Cluster I (Rps1-a), Cluster II (Rps1-b, 1-c and 1-k) and Cluster III (Rps3-a, 3-b, 3-c, 4, 5, and 6), suggesting an overlap in Rps-induced defense signaling among certain NILs. Gene ontology (GO) analysis revealed associations between members of the WRKY family and incompatible reactions and between a number of phytohormone signaling pathways and incompatible/compatible interactions. These associations appear to be distinguished according to the NIL clusters. Conclusions: This study characterized genes and multiple branches of putative regulatory networks associated with resistance to P. sojae in ten soybean NILs, and depicted functional "fingerprints" of individual Rps-mediated resistance responses through comparative transcriptomic analysis. Of particular interest are dramatic variations of detected DEGs, putatively involved in ethylene (ET)-, jasmonic acid (JA)-, (reactive oxygen species) ROS-, and (MAP-kinase) MAPK-signaling, among these soybean NILs, implicating their important roles of these signaling in differentiating molecular defense responses. We hypothesize that different timing and robustness in defense signaling to the same pathogen may be largely responsible for such variations. C1 [Lin, Feng; Zhao, Meixia; Ping, Jieqing; Sun, Lianjun; Liu, Yunfeng; Zhang, Biao; Tang, Zongxiang; Hughes, Elisa; Ma, Jianxin] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Baumann, Douglas D.] Univ Wisconsin, Dept Math, La Crosse, WI 54601 USA. [Doerge, Rebecca W.] Purdue Univ, Dept Stat, W Lafayette, IN 47907 USA. [Hughes, Teresa J.] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Hughes, Teresa J.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Hughes, TJ (reprint author), Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. EM hughestj@purdue.edu; maj@purdue.edu RI ZHAO, MEIXIA/N-3124-2015; Lin, Feng/K-1814-2016 OI ZHAO, MEIXIA/0000-0001-8812-8217; Lin, Feng/0000-0001-7491-4444 FU Indiana Soybean Alliance [205267]; U.S. Department of Agriculture's National Institute of Food and Agriculture; Ag Alumni Seed; AgReliant Genetics; Beck's Hybrids; ConAgraFoods; Dow AgroSciencesIndiana Crop Improvement Association; Pioneer Hi-Bred International FX This work was mainly supported by soybean checkoff funds from the Indiana Soybean Alliance (Grant No. 205267), and partially supported by "Partnership for Research & Education in Plant Breeding and Genetics" program funded by the U.S. Department of Agriculture's National Institute of Food and Agriculture, and corporate partners Ag Alumni Seed, AgReliant Genetics, Beck's Hybrids, ConAgraFoods, Dow AgroSciences, Indiana Crop Improvement Association and Pioneer Hi-Bred International. We also thank Brittany Radke, Tomara J. Fleury and Brian Foss for their help in inoculation and preparation of inoculum. NR 66 TC 8 Z9 9 U1 6 U2 53 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JAN 10 PY 2014 VL 15 AR 18 DI 10.1186/1471-2164-15-18 PG 13 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 288MI UT WOS:000329615700002 PM 24410936 ER PT J AU Lowell, EC Todoroki, CL Dykstra, DP Briggs, DG AF Lowell, Eini C. Todoroki, Christine L. Dykstra, Dennis P. Briggs, David G. TI Linking acoustic velocity of standing Douglas-fir trees to veneer stiffness: a tree-log-product study across thinning treatments SO NEW ZEALAND JOURNAL OF FORESTRY SCIENCE LA English DT Article DE Modulus of elasticity; Wood density; Stand density; Tree breeding; Veneer; Acoustic velocity; Douglas-fir ID WOOD DENSITY; QUALITY; GROWTH; ELASTICITY; SOFTWOODS; MODULUS; CROWN; PINE; SITE AB Background: To understand the effects of silvicultural treatments on tree and product modulus of elasticity (MOE), an intensive study along the stand-tree-product value chain was conducted. Acoustic tests were performed on 460 Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) trees from two sites in the US Pacific Northwest. Methods: Trees on a site in Washington were 51 years old, while those situated in Oregon were 45 years old. Both sites comprised five plots: Plot A was the control, while Plots B, C, D, and E were prescribed with differing thinning treatments. Acoustic readings were stratified, and a sample of six trees per plot randomly selected from the stratified samples. Trees were harvested, cross-cut, and peeled into veneer. Veneer was acoustically tested, and density and MOE calculated for each sheet. Veneer sheets were grouped by parent bolt and tree, and MOE of the butt veneer bolt and the parent tree determined from the mean. Plot means were compared using one-way analysis of variance (ANOVA), and regression models developed with mean tree MOE as the response variable and non-destructive tree measures as explanatory variables. Further models were developed to demonstrate the relationship between tree diameter and the number of veneer sheets peeled, and between MOE of the parent tree and butt bolt. Results: MOE was negatively correlated with diameter and taper, and positively correlated with acoustic velocity, V. The relationship with V-2, following the wave equation (MOE = rho V-2) under the assumption of constant density,rho, was no better than that with V. While the correlations suggest that thinning regimes that cause rapid diameter growth and increased taper may be susceptible to a reduction in overall stiffness, there was no evidence, as determined by ANOVA, to suggest that thinning reduces stiffness. On the contrary, mean MOE at plot E (light thinning) was 12.6 GPa and the control was 10.2 GPa, with the difference significant at the 10% level (p-value = 0.074). Conclusions: In general, trees with MOE exceeding the recommended tree breeding value of 11 GPa were of low taper and small diameter, suggesting that regimes with a lesser impact on taper and diameter, as demonstrated by Plot E, are worthy of further investigation. C1 [Lowell, Eini C.] US Forest Serv, Portland Forestry Sci Lab, Portland, OR 97205 USA. [Todoroki, Christine L.] New Zealand Forest Res Inst Ltd Scion, Rotorua 3046, New Zealand. [Dykstra, Dennis P.] Blue Ox Forestry, Paso Robles, CA 93446 USA. [Briggs, David G.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98101 USA. RP Lowell, EC (reprint author), US Forest Serv, Portland Forestry Sci Lab, 620 SW Main,Suite 400, Portland, OR 97205 USA. EM elowell@fs.fed.us FU Sustainable Forest Productivity component of Agenda; USDA Forest Service Research Development; American Forest and Paper Association; USFS Rocky Mountain Research Station; University of Washington, School of Environmental and Forest Sciences; USFS Pacific Northwest Research Station; USFS Forest Products Laboratory; New Zealand Forest Research Institute Limited, trading as Scion; USDA Forest Service, and through Future Forests Research Limited FX The authors thank the contributions of member partners of the Stand Management and Precision Forestry Cooperatives, the Rural Technology Initiative Program at the University of Washington's School of Environmental and Forest Sciences, the USFS Pacific Northwest Research Station, and CHH FibreGen. We also acknowledge statistical advice provided by Mark Kimberley of Scion and suggestions from anonymous reviewers. This research effort was supported by the Sustainable Forest Productivity component of Agenda 2020, a joint effort of the USDA Forest Service Research & Development and the American Forest and Paper Association. Funds were provided by the USFS Rocky Mountain Research Station, the University of Washington, School of Environmental and Forest Sciences, the USFS Pacific Northwest Research Station, and the USFS Forest Products Laboratory. In addition, research cumulating in this publication was supported by a Joint Venture Agreement between the New Zealand Forest Research Institute Limited, trading as Scion, and the USDA Forest Service, and through Future Forests Research Limited, a research partnership between Scion and New Zealand forest industries. NR 56 TC 3 Z9 3 U1 1 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0048-0134 EI 1179-5395 J9 NZ J FORESTRY SCI JI N. Z. J. Forest. Sci. PD JAN 9 PY 2014 VL 44 AR 1 DI 10.1186/1179-5395-44-1 PG 16 WC Forestry SC Forestry GA AJ0QE UT WOS:000337359500001 ER PT J AU Livingston, KA Rosen, JB Zucker, JR Zimmerman, CM AF Livingston, Kara A. Rosen, Jennifer B. Zucker, Jane R. Zimmerman, Christopher M. TI Mumps vaccine effectiveness and risk factors for disease in households during an outbreak in New York City SO VACCINE LA English DT Article DE Mumps; Outbreaks; Vaccine effectiveness; Households; Risk factors ID UNITED-STATES; POPULATION; ANTIBODY; STUDENTS; IMMUNITY AB Background and objectives: Mumps outbreaks have been reported among vaccinated populations, and declining mumps vaccine effectiveness (VE) has been suggested as one possible cause. During a large mumps outbreak in New York City, we assessed: (1) VE of measles-mumps-rubella vaccine (MMR) against mumps and (2) risk factors for acquiring mumps in households. Methods: Cases of mumps were investigated using standard methods. Additional information on disease and vaccination status of household contacts was collected. Case households completed follow-up phone interviews 78-198 days after initial investigation to ascertain additional cases. Mumps cases meeting the study case definition were included in the analysis. Risk factors for mumps were assessed, and VE was calculated using secondary household attack rates. Results: Three hundred and eleven households with 2176 residents were included in the analysis. The median age of residents was 13 years (range <1-85), and 462 (21.2%) residents met the study mumps case definition. Among 7-17 year olds, 89.7% received one or more doses of MMR vaccine, with 76.7% receiving two doses. Young adults aged 10-14 years (OR = 2.4, CI = 1.3-4.7) and 15-19 years (OR = 2.5, CI = 1.3-5.0) were at highest risk of mumps. The overall 2-dose VE for secondary contacts aged five and older was 86.3% (CI 63.3-94.9). Conclusions: The two-dose effectiveness of MMR vaccine against mumps was 86.3%, consistent with other published mumps VE estimates. Many factors likely contributed to this outbreak. Suboptimal MMR coverage in the affected population combined with VE may not have conferred adequate immunity to prevent transmission and may have contributed to this outbreak. Achieving high MMR coverage remains the best available strategy for prevention of mumps outbreaks. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Livingston, Kara A.] Ctr Dis Control, Council State & Terr Epidemiologists Appl Epidemi, Atlanta, GA 30333 USA. [Rosen, Jennifer B.; Zucker, Jane R.; Zimmerman, Christopher M.] New York City Dept Hlth & Mental Hyg, Bur Immunizat, New York, NY USA. [Zucker, Jane R.] Ctr Dis Control & Prevent, Immunizat Serv Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USA. RP Livingston, KA (reprint author), Tufts Univ, Jean Meyer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM kara.livingston@tufts.edu FU Centers for Disease Control and Prevention (CDC) [5U38HM000414] FX All authors contributed to the conception and design of the study, analysis design, and interpretation of data; KAL performed the statistical analysis and drafted the manuscript; all authors were involved in critical revision and had final approval of the manuscript. Conflict of interest statement: The authors declare no conflicts of interest. Funding: This work was supported in part by an appointment to the Applied Epidemiology Fellowship Program administered by the Council of State and Territorial Epidemiologists (CSTE) and funded by the Centers for Disease Control and Prevention (CDC) [Cooperative Agreement Number 5U38HM000414]. NR 40 TC 7 Z9 7 U1 0 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD JAN 9 PY 2014 VL 32 IS 3 BP 369 EP 374 DI 10.1016/j.vaccine.2013.11.021 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 297NE UT WOS:000330261400009 PM 24252695 ER PT J AU Cameron, EZ Edwards, AM White, AM AF Cameron, Elissa Z. Edwards, Amy M. White, Angela M. TI Halt self-citation in impact measures SO NATURE LA English DT Letter C1 [Cameron, Elissa Z.; Edwards, Amy M.] Univ Tasmania, Hobart, Tas, Australia. [White, Angela M.] US Forest Serv, USDA, Davis, CA USA. RP Cameron, EZ (reprint author), Univ Tasmania, Hobart, Tas, Australia. EM elissa.cameron@utas.edu.au RI Cameron, Elissa/B-8053-2014 OI Cameron, Elissa/0000-0002-9243-0547 NR 2 TC 1 Z9 1 U1 6 U2 28 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD JAN 9 PY 2014 VL 505 IS 7482 BP 160 EP 160 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 286CG UT WOS:000329441500019 PM 24402270 ER PT J AU Romera, SA Puntel, M Quattrocchi, V Zajac, PD Zamorano, P Viera, JB Carrillo, C Chowdhury, S Borca, MV Sadir, AM AF Alejandra Romera, Sonia Puntel, Mariana Quattrocchi, Valeria Del Medico Zajac, Paula Zamorano, Patricia Blanco Viera, Javier Carrillo, Consuelo Chowdhury, Shafiqul Borca, Manuel V. Sadir, Ana M. TI Protection induced by a glycoprotein E-deleted bovine herpesvirus type 1 marker strain used either as an inactivated or live attenuated vaccine in cattle SO BMC VETERINARY RESEARCH LA English DT Article DE BoHV-1 Delta gE beta gal; BoHV-1 marker vaccine; Inactivated/live attenuated vaccine; Bovines ID E-NEGATIVE STRAIN; TO-CELL SPREAD; GE GENE; VIRUS; BHV-1; RHINOTRACHEITIS; IMMUNOGENICITY; CALVES; CONSTRUCTION; PATHOGENESIS AB Background: Bovine herpesvirus type 1 (BoHV-1) is the causative agent of respiratory and genital tract infections; causing a high economic loss in all continents. Use of marker vaccines in IBR eradication programs is widely accepted since it allows for protection of the animals against the disease while adding the possibility of differentiating vaccinated from infected animals. The aim of the present study was the development and evaluation of safety and efficacy of a glycoprotein E-deleted (gE-) BoHV-1 marker vaccine strain (BoHV-1 Delta gE beta gal) generated by homologous recombination, replacing the viral gE gene with the beta-galactosidase (beta gal) gene. Results: In vitro growth kinetics of the BoHV-1 Delta gE beta gal virus was similar to BoHV-1 LA. The immune response triggered by the new recombinant strain in cattle was characterized both as live attenuated vaccine (LAV) and as an inactivated vaccine. BoHV-1 Delta gE beta gal was highly immunogenic in both formulations, inducing specific humoral and cellular immune responses. Antibody titers found in animals vaccinated with the inactivated vaccine based on BoHV-1 Delta gE beta gal was similar to the titers found for the control vaccine (BoHV-1 LA). In the same way, titers of inactivated vaccine groups were significantly higher than any of the LAV immunized groups, independently of the inoculation route (p < 0.001). Levels of IFN-gamma were significantly higher (p < 0.001) in those animals that received the LAV compared to those that received the inactivated vaccine. BoHV-1 Delta gE beta gal exhibited an evident attenuation when administered as a LAV; no virus was detected in nasal secretions of vaccinated or sentinel animals during the post-vaccination period. BoHV-1 Delta gE beta gal, when used in either formulation, elicited an efficient immune response that protected animals against challenge with virulent wild-type BoHV-1. Also, the deletion of the gE gene served as an immunological marker to differentiate vaccinated animals from infected animals. All animals vaccinated with the BoHV-1 Delta gE beta gal strain were protected against disease after challenge and shed significantly less virus than control calves, regardless of the route and formulation they were inoculated. Conclusions: Based on its attenuation, immunogenicity and protective effect after challenge, BoHV-1 Delta gE beta gal virus is an efficient and safe vaccine candidate when used either as inactivated or as live attenuated forms. C1 [Alejandra Romera, Sonia; Quattrocchi, Valeria; Del Medico Zajac, Paula; Zamorano, Patricia; Sadir, Ana M.] INTA, CICV, Inst Virol, RA-1708 Moron, Argentina. [Puntel, Mariana] Consejo Nacl Invest Cient & Tecn, Fdn Inst Leloir IIBBA, RA-1405 Caba, Argentina. [Blanco Viera, Javier] INTA, Inst Patobiol CICVyA, RA-1708 Moron, Argentina. [Carrillo, Consuelo] USDA, Plum Isl Anim Dis Ctr, APHIS, Greenport, NY 11944 USA. [Chowdhury, Shafiqul] Louisiana State Univ, Sch Vet Med, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA. [Borca, Manuel V.] USDA ARS, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Alejandra Romera, Sonia; Zamorano, Patricia; Sadir, Ana M.] Univ Salvador, Buenos Aires, DF, Argentina. [Alejandra Romera, Sonia; Zamorano, Patricia; Sadir, Ana M.] Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina. RP Romera, SA (reprint author), INTA, CICV, Inst Virol, CC77, RA-1708 Moron, Argentina. EM aromera@cnia.inta.gov.ar OI Borca, Manuel/0000-0002-0888-1178 FU SECYT- INTA-San Jorge Bago, Argentina [053] FX Special thanks to Dr. F. Maliandi for his constant support during the project. This research was supported by grant BID 802 OC-AR PMT/PIT no 053 from SECYT- INTA-San Jorge Bago, Argentina. We particularly thank Melanie Prarat and Dr. Mariano Perez Filgueira for the editing of the manuscript. NR 36 TC 6 Z9 7 U1 1 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD JAN 8 PY 2014 VL 10 AR 8 DI 10.1186/1746-6148-10-8 PG 12 WC Veterinary Sciences SC Veterinary Sciences GA 294US UT WOS:000330073600001 PM 24401205 ER PT J AU Baruch-Mordo, S Wilson, KR Lewis, DL Broderick, J Mao, JS Breck, SW AF Baruch-Mordo, Sharon Wilson, Kenneth R. Lewis, David L. Broderick, John Mao, Julie S. Breck, Stewart W. TI Stochasticity in Natural Forage Production Affects Use of Urban Areas by Black Bears: Implications to Management of Human-Bear Conflicts SO PLOS ONE LA English DT Article ID AMELANCHIER-ALNIFOLIA ROSACEAE; URSUS-AMERICANUS; POPULATION-DYNAMICS; EVOLUTIONARY TRAPS; COLLAR PERFORMANCE; ACTIVITY PATTERNS; NATIONAL-PARK; HABITAT USE; HOME-RANGE; SURVIVAL AB The rapid expansion of global urban development is increasing opportunities for wildlife to forage and become dependent on anthropogenic resources. Wildlife using urban areas are often perceived dichotomously as urban or not, with some individuals removed in the belief that dependency on anthropogenic resources is irreversible and can lead to increased human-wildlife conflict. For American black bears (Ursus americanus), little is known about the degree of bear urbanization and its ecological mechanisms to guide the management of human-bear conflicts. Using 6 years of GPS location and activity data from bears in Aspen, Colorado, USA, we evaluated the degree of bear urbanization and the factors that best explained its variations. We estimated space use, activity patterns, survival, and reproduction and modeled their relationship with ecological covariates related to bear characteristics and natural food availability. Space use and activity patterns were dependent on natural food availability (good or poor food years), where bears used higher human density areas and became more nocturnal in poor food years. Patterns were reversible, i.e., individuals using urban areas in poor food years used wildland areas in subsequent good food years. While reproductive output was similar across years, survival was lower in poor food years when bears used urban areas to a greater extent. Our findings suggest that bear use of urban areas is reversible and fluctuates with the availability of natural food resources, and that removal of urban individuals in times of food failures has the potential to negatively affect bear populations. Given that under current predictions urbanization is expected to increase by 11% across American black bear range, and that natural food failure years are expected to increase in frequency with global climate change, alternative methods of reducing urban human-bear conflict are required if the goal is to prevent urban areas from becoming population sinks. C1 [Baruch-Mordo, Sharon; Wilson, Kenneth R.; Lewis, David L.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Baruch-Mordo, Sharon; Wilson, Kenneth R.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Broderick, John] Colorado Pk & Wildlife, Terr Programs, Ft Collins, CO USA. [Mao, Julie S.] Colorado Pk & Wildlife, Terr Programs, Glenwood Springs, CO USA. [Breck, Stewart W.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Ft Collins, CO USA. RP Baruch-Mordo, S (reprint author), Nature Conservancy, Dev Design, Ft Collins, CO USA. EM sbaruch-mordo@tnc.org FU United States Department of Agriculture - National Wildlife Research Center; city of Aspen [5353530]; Aspen Field Biology Lab; Jack H. Berryman Institute; Rocky Mountain Goats Foundation; Colorado Chapter of The Wildlife Society FX This study was funded by the United States Department of Agriculture - National Wildlife Research Center (SWB) (http://www.aphis.usda.gov/wildlife_damage/nwrc/). Additional funds awarded by the city of Aspen to KRW (CSU account no. 5353530). Fellowship and scholarship support given to SBM by Aspen Field Biology Lab, Jack H. Berryman Institute, Rocky Mountain Goats Foundation, and the Colorado Chapter of The Wildlife Society. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 79 TC 14 Z9 14 U1 15 U2 82 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 8 PY 2014 VL 9 IS 1 AR e85122 DI 10.1371/journal.pone.0085122 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 291WL UT WOS:000329862500218 PM 24416350 ER PT J AU Campbell, C Grapov, D Fiehn, O Chandler, CJ Burnett, DJ Souza, EC Casazza, GA Gustafson, MB Keim, NL Newman, JW Hunter, GR Fernandez, JR Garvey, WT Harper, ME Hoppel, CL Meissen, JK Take, K Adams, SH AF Campbell, Caitlin Grapov, Dmitry Fiehn, Oliver Chandler, Carol J. Burnett, Dustin J. Souza, Elaine C. Casazza, Gretchen A. Gustafson, Mary B. Keim, Nancy L. Newman, John W. Hunter, Gary R. Fernandez, Jose R. Garvey, W. Timothy Harper, Mary-Ellen Hoppel, Charles L. Meissen, John K. Take, Kohei Adams, Sean H. TI Improved Metabolic Health Alters Host Metabolism in Parallel with Changes in Systemic Xeno-Metabolites of Gut Origin SO PLOS ONE LA English DT Article ID GLUCOSE-TOLERANCE TEST; INSULIN-RESISTANCE; TRICARBALLYLIC ACID; MENSTRUAL-CYCLE; HUMAN URINE; PLASMA; MOUSE; SENSITIVITY; PROFILES; OBESITY AB Novel plasma metabolite patterns reflective of improved metabolic health (insulin sensitivity, fitness, reduced body weight) were identified before and after a 14-17 wk weight loss and exercise intervention in sedentary, obese insulin-resistant women. To control for potential confounding effects of diet- or microbiome-derived molecules on the systemic metabolome, sampling was during a tightly-controlled feeding test week paradigm. Pairwise and multivariate analysis revealed intervention-and insulin-sensitivity associated: (1) Changes in plasma xeno-metabolites ("non-self'' metabolites of dietary or gut microbial origin) following an oral glucose tolerance test (e.g. higher post-OGTT propane-1,2,3-tricarboxylate [tricarballylic acid]) or in the overnight-fasted state (e.g., lower gamma-tocopherol); (2) Increased indices of saturated very long chain fatty acid elongation capacity; (3) Increased post-OGTT alpha-ketoglutaric acid (alpha-KG), fasting alpha-KG inversely correlated with Matsuda index, and altered patterns of malate, pyruvate and glutamine hypothesized to stem from improved mitochondrial efficiency and more robust oxidation of glucose. The results support a working model in which improved metabolic health modifies host metabolism in parallel with altering systemic exposure to xeno-metabolites. This highlights that interpretations regarding the origins of peripheral blood or urinary "signatures'' of insulin resistance and metabolic health must consider the potentially important contribution of gut-derived metabolites toward the host's metabolome. C1 [Campbell, Caitlin; Chandler, Carol J.; Burnett, Dustin J.; Souza, Elaine C.; Gustafson, Mary B.; Keim, Nancy L.; Newman, John W.; Adams, Sean H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Grapov, Dmitry; Fiehn, Oliver] Univ Calif Davis, West Coast Metabol Ctr, Davis, CA USA. [Fiehn, Oliver; Meissen, John K.; Take, Kohei] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Casazza, Gretchen A.] Univ Calif Davis, Sch Med, Sports Med Program, Sacramento, CA 95817 USA. [Keim, Nancy L.; Newman, John W.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Hunter, Gary R.; Fernandez, Jose R.; Garvey, W. Timothy] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA. [Hunter, Gary R.] Univ Alabama Birmingham, Human Studies Dept, Birmingham, AL USA. [Harper, Mary-Ellen] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON, Canada. [Hoppel, Charles L.] Case Western Reserve Univ, Dept Pharmacol, Cleveland, OH 44106 USA. RP Fiehn, O (reprint author), Univ Calif Davis, West Coast Metabol Ctr, Davis, CA USA. EM ofiehn@ucdavis.edu; sean.h.adams@ars.usda.gov OI Harper, Mary-Ellen/0000-0003-3864-5886 FU USDA-ARS intramural [5306-51530-016-00D, 5306-51530-019-00]; NIH-NIDDK [R01DK078328]; NIH [1 U24 DK097154, P20 HL113452]; West Coast Metabolomics Center FX This work was supported by USDA-ARS intramural Projects 5306-51530-016-00D and 5306-51530-019-00 (JWN, NLK, SHA), NIH-NIDDK R01DK078328 (OF, NLK, JWN, GH, JF, WTG, CLH, MEH, SHA), NIH 1 U24 DK097154 for the West Coast Metabolomics Center (OF, JWN), and NIH P20 HL113452 (OF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 71 TC 19 Z9 19 U1 2 U2 32 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 8 PY 2014 VL 9 IS 1 AR e84260 DI 10.1371/journal.pone.0084260 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 291WL UT WOS:000329862500106 PM 24416208 ER PT J AU Gladue, DP Baker-Bransetter, R Holinka, LG Fernandez-Sainz, IJ O'Donnell, V Fletcher, P Lu, ZQ Borca, MV AF Gladue, Douglas P. Baker-Bransetter, Ryan Holinka, Lauren G. Fernandez-Sainz, Ignacio J. O'Donnell, Vivian Fletcher, Paige Lu, Zhiqiang Borca, Manuel V. TI Interaction of CSFV E2 Protein with Swine Host Factors as Detected by Yeast Two-Hybrid System SO PLOS ONE LA English DT Article ID VIRAL-DIARRHEA-VIRUS; ENVELOPE GLYCOPROTEIN E2; FEVER VIRUS; E-RNS; DENDRITIC CELLS; VIRULENCE DETERMINANT; INFLUENZA-VIRUS; STRAIN BRESCIA; CORE PROTEIN; N-PRO AB E2 is one of the envelope glycoproteins of pestiviruses, including classical swine fever virus (CSFV) and bovine viral diarrhea virus (BVDV). E2 is involved in several critical functions, including virus entry into target cells, induction of a protective immune response and virulence in swine. However, there is no information regarding any host binding partners for the E2 proteins. Here, we utilized the yeast two-hybrid system and identified fifty-seven host proteins as positive binding partners which bound E2 from both CSFV and BVDV with the exception of two proteins that were found to be positive for binding only to CSFV E2. Alanine scanning of CSFV E2 demonstrated that the binding sites for these cellular proteins on E2 are likely non-linear binding sites. The possible roles of the identified host proteins are discussed as the results presented here will be important for future studies to elucidate mechanisms of host protein-virus interactions during pestivirus infection. However, due to the limitations of the yeast two hybrid system, the proteins identified is not exhaustive and each interaction identified needs to be confirmed by independent experimental approaches in the context of virus-infected cells before any definitive conclusion can be drawn on relevance for the virus life cycle. C1 [Gladue, Douglas P.; Baker-Bransetter, Ryan; Holinka, Lauren G.; Fernandez-Sainz, Ignacio J.; O'Donnell, Vivian; Fletcher, Paige; Borca, Manuel V.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Gladue, Douglas P.; O'Donnell, Vivian] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT USA. [Lu, Zhiqiang] USDA ARS, Plum Isl Anim Dis Ctr, Dept Homeland Secur, Greenport, NY 11944 USA. RP Borca, MV (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. EM manuel.borca@ars.usda.gov RI Fernandez Sainz, Ignacio/I-3046-2016; OI Gladue, Douglas/0000-0002-7894-0233; Borca, Manuel/0000-0002-0888-1178 FU USDA FX Funded by the USDA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 67 TC 8 Z9 8 U1 0 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 8 PY 2014 VL 9 IS 1 AR e85324 DI 10.1371/journal.pone.0085324 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 291WL UT WOS:000329862500240 PM 24416391 ER PT J AU Jin, TC Wang, Y Chen, YW Fu, TJ Kothary, MH McHugh, TH Zhang, YZ AF Jin, Tengchuan Wang, Yang Chen, Yu-Wei Fu, Tong-Jen Kothary, Mahendra H. McHugh, Tara H. Zhang, Yuzhu TI Crystal Structure of Korean Pine (Pinus koraiensis) 7S Seed Storage Protein with Copper Ligands SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE 7S vicilin; pine nut; allergen; X-ray crystallography; copper protein ID MAJOR PEANUT ALLERGEN; ARA H 1; TREE NUT ALLERGENS; CHEMICAL-COMPOSITION; MOLECULAR GRAPHICS; FOOD ALLERGY; PROGRAM; VICILIN; CRYSTALLIZATION; PURIFICATION AB The prevalence of food allergy has increased in recent years, and Korean pine vicilin is a potential food allergen. We have previously reported the crystallization of Korean pine vicilin purified from raw pine nut. Here we report the isolation of vicilin mRNA and the crystal structure of Korean pine vicilin at 2.40 angstrom resolution. The overall structure of pine nut vicilin is similar to the structures of other 7S seed storage proteins and consists of an N-terminal domain and a C-terminal domain. Each assumes a cupin fold, and they are symmetrically related about a pseudodyad axis. Three vicilin molecules form a doughnut-shaped trimer through head-to-tail association. Structure characterization of Korean pine nut vicilin unexpectedly showed that, in its native trimeric state, the vicilin has three copper ligands. Sequence alignments suggested that the copper-coordinating residues were conserved in winter squash, sesame, tomato, and several tree nuts, while they were not conserved in a number of legumes, including peanut and soybean. Additional studies are needed to assess whether the copper-coordinating property of vicilins has a biological function in the relevant plants. The nutritional value of this copper-coordinating protein in tree nuts and other edible seeds may be worth further investigations. C1 [Jin, Tengchuan; Wang, Yang; Chen, Yu-Wei] IIT, Dept Biol, Chicago, IL 60616 USA. [Fu, Tong-Jen] US FDA, Ctr Food Safety & Appl Nutr, Bedford Pk, IL 60501 USA. [Kothary, Mahendra H.] US FDA, Ctr Food Safety & Appl Nutr, Laurel, MD 20708 USA. [McHugh, Tara H.; Zhang, Yuzhu] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Jin, TC (reprint author), NIAID, Struct Immunobiol Unit, Immunol Lab, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM tengchuan@gmail.com; yuzhu.zhang@ars.usda.gov RI Jin, Tengchuan/B-5883-2014; OI Jin, Tengchuan/0000-0002-1395-188X; Zhang, Yuzhu/0000-0001-7882-5692 FU U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [W-31-109-Eng-38]; Illinois Institute of Technology; U.S. Food and Drug Administration [5U01FD003801]; Institute for Food Safety and Health, Illinois Institute of Technology [5U01FD003801] FX Use of the APS was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract W-31-109-Eng-38. This work was partially supported by a fund from the Illinois Institute of Technology and by Cooperative Agreement 5U01FD003801 between the U.S. Food and Drug Administration and Institute for Food Safety and Health, Illinois Institute of Technology. NR 55 TC 7 Z9 7 U1 3 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 8 PY 2014 VL 62 IS 1 BP 222 EP 228 DI 10.1021/jf4039887 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 288BK UT WOS:000329586800028 PM 24328105 ER PT J AU Truong, VD Pascua, YT Reynolds, R Thompson, RL Palazoglu, TK Mogol, BA Gokmen, V AF Van-Den Truong Pascua, Yvette T. Reynolds, Rong Thompson, Roger L. Palazoglu, T. Koray Mogol, Burce Atac Gokmen, Vural TI Processing Treatments for Mitigating Acrylamide Formation in Sweetpotato French Fries SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE sweet potatoes; Ipomoea batatas; sugars; asparagine; acrylamide French fries ID MAILLARD REACTION; TEMPERATURE; REDUCTION; PRODUCTS; CHIPS; CULTIVARS; TUBERS; FUTURE; SUGARS; LEVEL AB Acrylamide formation in sweetpotato French fries (SPFF) is likely a potential health concern as there is an increasing demand for good-quality fries from carotene-rich sweetpotatoes (SP). This is the first report on acrylamide formation in SPFF as affected by processing methods. Acrylamide levels in SPFF from untreated SP strips fried at 165 degrees C for 2, 3, and 5 mm were 124.9, 255.5, and 452.0 ng/g fresh weight, which were reduced by about 7 times to 16.3, 36.9, and 58.3 ng/g, respectively, when the strips were subjected to processing that included water blanching and soaking in 0.5% sodium acid pyrophosphate before frying. An additional step of strip soaking in 0.4% calcium chloride solution before par-frying increased the calcium content from 0.2 to 0.8 mg/g and decreased the acrylamide levels to 6.3, 17.6, and 35.4 ng/g, respectively. SPFF with acrylamide level of <100 ng/g or several times lower than that of white potato French fries can be obtained by integrating processing treatments commonly used in the food industry. C1 [Van-Den Truong; Pascua, Yvette T.; Reynolds, Rong; Thompson, Roger L.] N Carolina State Univ, USDA ARS, SAA Food Sci Res Unit, Raleigh, NC 27695 USA. [Palazoglu, T. Koray] Mersin Univ, Dept Food Engn, TR-33343 Ciftlikkoy, Mersin, Turkey. [Mogol, Burce Atac; Gokmen, Vural] Hacettepe Univ, Dept Food Engn, TR-06532 Ankara, Turkey. RP Truong, VD (reprint author), N Carolina State Univ, USDA ARS, SAA Food Sci Res Unit, Raleigh, NC 27695 USA. EM den.truong@ars.usda.gov RI Atac Mogol, Burce/B-3233-2013; Gokmen, Vural/G-5478-2011; Palazoglu, Tunc/I-7780-2015 OI Atac Mogol, Burce/0000-0002-1117-030X; Gokmen, Vural/0000-0002-9601-5391; NR 44 TC 5 Z9 7 U1 10 U2 61 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 8 PY 2014 VL 62 IS 1 BP 310 EP 316 DI 10.1021/jf404290v PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 288BK UT WOS:000329586800039 PM 24328312 ER PT J AU Schaberg, PG Hawley, GJ Rayback, SA Halman, JM Kosiba, AM AF Schaberg, Paul G. Hawley, Gary J. Rayback, Shelly A. Halman, Joshua M. Kosiba, Alexandra M. TI Inconclusive evidence of Juniperus virginiana recovery following sulfur pollution reductions SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Letter C1 [Schaberg, Paul G.] US Forest Serv, No Res Stn, Burlington, VT 05405 USA. [Hawley, Gary J.; Halman, Joshua M.; Kosiba, Alexandra M.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Rayback, Shelly A.] Univ Vermont, Dept Geog, Burlington, VT 05405 USA. RP Schaberg, PG (reprint author), US Forest Serv, No Res Stn, Burlington, VT 05405 USA. EM pschaberg@fs.fed.us NR 5 TC 1 Z9 1 U1 2 U2 8 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN 7 PY 2014 VL 111 IS 1 BP E1 EP E1 DI 10.1073/pnas.1320526111 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 284VY UT WOS:000329350700001 PM 24374634 ER PT J AU Corella, D Sorli, JV Gonzlez, JI Ortega, C Fit, M Bull, M Martinez-Gonzlez, MA Ros, E Ars, F Lapetra, J Gmez-Gracia, E Serra-Majem, L Ruiz-Gutierrez, V Fiol, M Coltell, O Vinyoles, E Pinto, X Marti, A Saiz, C Ordovs, JM Estruch, R AF Corella, Dolores Sorli, Jose V. Gonzlez, Jose I. Ortega, Carolina Fit, Montserrat Bull, Monica Martinez-Gonzlez, Miguel Angel Ros, Emilio Ars, Fernando Lapetra, Jose Gmez-Gracia, Enrique Serra-Majem, Lluis Ruiz-Gutierrez, Valentina Fiol, Miquel Coltell, Oscar Vinyoles, Ernest Pinto, Xavier Marti, Amelia Saiz, Carmen Ordovs, Jose M. Estruch, Ramn TI Novel association of the obesity risk-allele near Fas Apoptotic Inhibitory Molecule 2 (FAIM2) gene with heart rate and study of its effects on myocardial infarction in diabetic participants of the PREDIMED trial SO CARDIOVASCULAR DIABETOLOGY LA English DT Article DE Heart rate; FAIM2; Apoptosis ID BODY-MASS INDEX; GENOME-WIDE ASSOCIATION; CORONARY-ARTERY-DISEASE; CARDIOVASCULAR-DISEASE; JAPANESE POPULATION; MEDITERRANEAN DIET; CAUSE MORTALITY; SOLUBLE FAS; VARIANTS; LOCI AB Background: The Fas apoptotic pathway has been implicated in type 2 diabetes and cardiovascular disease. Although a polymorphism (rs7138803; G > A) near the Fas apoptotic inhibitory molecule 2 (FAIM2) locus has been related to obesity, its association with other cardiovascular risk factors and disease remains uncertain. Methods: We analyzed the association between the FAIM2-rs7138803 polymorphism and obesity, blood pressure and heart rate in 7,161 participants (48.3% with type 2 diabetes) in the PREDIMED study at baseline. We also explored gene-diet interactions with adherence to the Mediterranean diet (MedDiet) and examined the effects of the polymorphism on cardiovascular disease incidence per diabetes status after a median 4.8-year dietary intervention (MedDiet versus control group) follow-up. Results: We replicated the association between the FAIM2-rs7138803 polymorphism and greater obesity risk (OR: 1.08; 95% CI: 1.01-1.16; P = 0.011; per-A allele). Moreover, we detected novel associations of this polymorphism with higher diastolic blood pressure (DBP) and heart rate at baseline (B = 1.07; 95% CI: 0.97-1.28 bmp in AA vs G-carriers for the whole population), that remained statistically significant even after adjustment for body mass index (P = 0.012) and correction for multiple comparisons. This association was greater and statistically significant in type-2 diabetic subjects (B = 1.44: 95% CI: 0.23-2.56 bmp; P = 0.010 for AA versus G-carriers). Likewise, these findings were also observed longitudinally over 5-year follow-up. Nevertheless, we found no statistically significant gene-diet interactions with MedDiet for this trait. On analyzing myocardial infarction risk, we detected a nominally significant (P = 0.041) association in type-2 diabetic subjects (HR: 1.86; 95% CI: 1.03-3.37 for AA versus G-carriers), although this association did not remain statistically significant following correction for multiple comparisons. Conclusions: We confirmed the FAIM2-rs7138803 relationship with obesity and identified novel and consistent associations with heart rate in particular in type 2 diabetic subjects. Furthermore, our results suggest a possible association of this polymorphism with higher myocardial infarction risk in type-2 diabetic subjects, although this result needs to be replicated as it could represent a false positive. C1 [Corella, Dolores; Sorli, Jose V.; Gonzlez, Jose I.; Ortega, Carolina; Saiz, Carmen] Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Valencia, Spain. [Corella, Dolores; Sorli, Jose V.; Gonzlez, Jose I.; Ortega, Carolina; Fit, Montserrat; Bull, Monica; Martinez-Gonzlez, Miguel Angel; Ros, Emilio; Ars, Fernando; Lapetra, Jose; Gmez-Gracia, Enrique; Serra-Majem, Lluis; Ruiz-Gutierrez, Valentina; Fiol, Miquel; Coltell, Oscar; Pinto, Xavier; Marti, Amelia; Estruch, Ramn] Inst Salud Carlos III, CIBER Fisiopatol Obesidad Nutr, Madrid, Spain. [Fit, Montserrat] Inst Hosp Mar Invest Med IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona, Spain. [Bull, Monica] Univ Rovira & Virgili, IISPV, Fac Med, Human Nutr Unit, Reus, Spain. [Martinez-Gonzlez, Miguel Angel] Univ Navarra, Sch Med, Dept Prevent Med & Publ Hlth, E-31080 Pamplona, Spain. [Ros, Emilio] Hosp Clin Barcelona, IDIBAPS, Endocrinol & Nutr Serv, Lipid Clin, Barcelona, Spain. [Ars, Fernando] Araba Univ Hosp, Dept Cardiol, Vitoria, Spain. [Lapetra, Jose] San Pablo Hlth Ctr, Primary Care Div Sevilla, Dept Family Med, Seville, Spain. [Gmez-Gracia, Enrique] Univ Malaga, Sch Med, Dept Epidemiol, Malaga, Spain. [Serra-Majem, Lluis] Univ Las Palmas Gran Canaria, Dept Clin Sci, Las Palmas Gran Canaria, Spain. [Ruiz-Gutierrez, Valentina] Consejo Super Invest Cientif, Inst Grasa, Seville, Spain. [Fiol, Miquel] Univ Inst Hlth Sci Invest, Hosp Son Dureta, Palma de Mallorca, Spain. [Coltell, Oscar] Jaume 1 Univ, Sch Technol & Expt Sci, Dept Comp Languages & Syst, Castellon de La Plana, Spain. [Vinyoles, Ernest] Catalan Inst Hlth, Primary Care Div, Barcelona, Spain. [Pinto, Xavier] Hosp Univ Bellvitge, Hosp Llobregat, Lipids & Vasc Risk Unit, Barcelona, Spain. [Marti, Amelia] Univ Navarra, Fac Pharm, Dept Nutr & Physiol, E-31080 Pamplona, Spain. [Ordovs, Jose M.] CNIC, Dept Cardiovasc Epidemiol & Populat Genet, Madrid, Spain. [Ordovs, Jose M.] IMDEA Alimentac, Madrid, Spain. [Ordovs, Jose M.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Estruch, Ramn] IDIBAPS, Hosp Clin, Dept Internal Med, Barcelona, Spain. [Corella, Dolores] Univ Valencia, Genet & Mol Epidemiol Unit, Valencia 46010, Spain. RP Corella, D (reprint author), Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Valencia, Spain. EM dolores.corella@uv.es RI Lapetra, Jose/F-2552-2015; Fiol, Miguel/F-6793-2016; IBIS, ATENCION PRIMARIA/I-3524-2016; Fito, Montserrat/C-1822-2012; OI Fito, Montserrat/0000-0002-1817-483X; Vinyoles, Ernest/0000-0002-8725-9431; Martinez-Gonzalez, Miguel A./0000-0002-3917-9808; Bullo, Monica/0000-0002-0218-7046; Marti, Amelia/0000-0001-9832-7981 FU Spanish Ministry of Health (Instituto de Salud Carlos III); Ministry of Economy and Innovation, Spain; Fondo Europeo de Desarrollo Regional [PI051839, PI070240, PI1001407, G03/140, CIBER 06/03, RD06/0045 PI07-0954, CNIC-06, PI11/02505, SAF2009-12304, AGL2010-22319-C03-03]; US Department of Agriculture Research, USA [53-K06-5-10, 58-1950-9-001]; Generalitat Valenciana, Spain [AP111/10, AP-042/11, BEST11-263, BEST/2011/261, GVACOMP2011-151, ACOMP/2011/145, ACOMP/2012/190] FX This study has been supported by The Spanish Ministry of Health (Instituto de Salud Carlos III) and the Ministry of Economy and Innovation, Spain and Fondo Europeo de Desarrollo Regional (projects PI051839, PI070240, PI1001407, G03/140, CIBER 06/03, RD06/0045 PI07-0954, CNIC-06, PI11/02505, SAF2009-12304 and AGL2010-22319-C03-03), by contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research, USA and by the Generalitat Valenciana, Spain (AP111/10, AP-042/11, BEST11-263, BEST/2011/261, GVACOMP2011-151, ACOMP/2011/145 and ACOMP/2012/190). NR 59 TC 3 Z9 4 U1 2 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1475-2840 J9 CARDIOVASC DIABETOL JI Cardiovasc. Diabetol. PD JAN 6 PY 2014 VL 13 AR 5 DI 10.1186/1475-2840-13-5 PG 13 WC Cardiac & Cardiovascular Systems; Endocrinology & Metabolism SC Cardiovascular System & Cardiology; Endocrinology & Metabolism GA AD0WF UT WOS:000332954300001 PM 24393375 ER PT J AU Costa-Hurtado, M Afonso, CL Miller, PJ Spackman, E Kapczynski, DR Swayne, DE Shepherd, E Smith, D Zsak, A Pantin-Jackwood, M AF Costa-Hurtado, Mar Afonso, Claudio L. Miller, Patti J. Spackman, Erica Kapczynski, Darrell R. Swayne, David E. Shepherd, Eric Smith, Diane Zsak, Aniko Pantin-Jackwood, Mary TI Virus interference between H7N2 low pathogenic avian influenza virus and lentogenic Newcastle disease virus in experimental co-infections in chickens and turkeys SO VETERINARY RESEARCH LA English DT Article ID EVALUATING VIRAL INTERFERENCE; INFECTIOUS-BRONCHITIS VIRUS; POLYMERASE CHAIN-REACTION; POULTRY; H9N2; DUCKS; SURVEILLANCE; VACCINATION; RESPONSES; STRAINS AB Low pathogenicity avian influenza virus (LPAIV) and lentogenic Newcastle disease virus (lNDV) are commonly reported causes of respiratory disease in poultry worldwide with similar clinical and pathobiological presentation. Co-infections do occur but are not easily detected, and the impact of co-infections on pathobiology is unknown. In this study chickens and turkeys were infected with a lNDV vaccine strain (LaSota) and a H7N2 LPAIV (A/turkey/VA/SEP-67/2002) simultaneously or sequentially three days apart. No clinical signs were observed in chickens co-infected with the lNDV and LPAIV or in chickens infected with the viruses individually. However, the pattern of virus shed was different with co-infected chickens, which excreted lower titers of lNDV and LPAIV at 2 and 3 days post inoculation (dpi) and higher titers at subsequent time points. All turkeys inoculated with the LPAIV, whether or not they were exposed to lNDV, presented mild clinical signs. Co-infection effects were more pronounced in turkeys than in chickens with reduction in the number of birds shedding virus and in virus titers, especially when LPAIV was followed by lNDV. In conclusion, co-infection of chickens or turkeys with lNDV and LPAIV affected the replication dynamics of these viruses but did not affect clinical signs. The effect on virus replication was different depending on the species and on the time of infection. These results suggest that infection with a heterologous virus may result in temporary competition for cell receptors or competent cells for replication, most likely interferon-mediated, which decreases with time. C1 [Costa-Hurtado, Mar; Afonso, Claudio L.; Miller, Patti J.; Spackman, Erica; Kapczynski, Darrell R.; Swayne, David E.; Shepherd, Eric; Smith, Diane; Zsak, Aniko; Pantin-Jackwood, Mary] USDA, Agr Res Serv, Southeast Poultry Res Lab, Exot & Emerging Avian Viral Dis Unit, Athens, GA USA. RP Pantin-Jackwood, M (reprint author), USDA, Agr Res Serv, Southeast Poultry Res Lab, Exot & Emerging Avian Viral Dis Unit, Athens, GA USA. EM Mary.Pantin-Jackwood@ars.usda.gov RI Costa-Hurtado, Mar/R-1496-2016 OI Costa-Hurtado, Mar/0000-0003-4194-0253 FU Agriculture Research Service CRIS [6612-32000-048]; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN266200700007C] FX The authors appreciate the assistance provided by Tim Olivier, Dawn Williams-Coplin, Rami Cha, Kira Moresco, Ronald Graham, and Roger Brock in conducting these studies, and also would like to thank Drs. David Stallknecht, Justin Brown and Monique Franca for helpful ideas. This work has been funded by the Agriculture Research Service CRIS Project 6612-32000-048 and with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN266200700007C. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. NR 50 TC 8 Z9 9 U1 0 U2 15 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD JAN 6 PY 2014 VL 45 AR 1 DI 10.1186/1297-9716-45-1 PG 11 WC Veterinary Sciences SC Veterinary Sciences GA 297SU UT WOS:000330276000001 PM 24393488 ER EF